LOCUS       DAJTQQ010000001       136028 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000001 DAJTQQ010000000
VERSION     DAJTQQ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 136028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 136028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..136028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.001"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..1065
                     /gene="cscA"
                     /locus_tag="OO309_000001"
     CDS             <1..1065
                     /gene="cscA"
                     /locus_tag="OO309_000001"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR5845316.1"
                     /translation="NDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFRDPKVWRE
                     ADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPDFFSLGDQ
                     HYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDFYAPQSFL
                     AKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEAESLRQQH
                     QSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDNQSERLIL
                     WRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYPQPEEREL
                     SLYASHGVAVLQHGALWLLG"
     gene            complement(1073..2131)
                     /locus_tag="OO309_000002"
     CDS             complement(1073..2131)
                     /locus_tag="OO309_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5845317.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2299..2517
                     /locus_tag="OO309_000003"
                     /pseudo
     CDS             2299..2517
                     /locus_tag="OO309_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            2535..3863
                     /gene="dsdA"
                     /locus_tag="OO309_000004"
     CDS             2535..3863
                     /gene="dsdA"
                     /locus_tag="OO309_000004"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR5845318.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(3971..5509)
                     /gene="emrY"
                     /locus_tag="OO309_000005"
     CDS             complement(3971..5509)
                     /gene="emrY"
                     /locus_tag="OO309_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR5845319.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(5509..6672)
                     /gene="emrK"
                     /locus_tag="OO309_000006"
     CDS             complement(5509..6672)
                     /gene="emrK"
                     /locus_tag="OO309_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR5845320.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7088..7702
                     /gene="evgA"
                     /locus_tag="OO309_000007"
     CDS             7088..7702
                     /gene="evgA"
                     /locus_tag="OO309_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR5845321.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            7707..11300
                     /gene="evgS"
                     /locus_tag="OO309_000008"
     CDS             7707..11300
                     /gene="evgS"
                     /locus_tag="OO309_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR5845322.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11356..12501)
                     /gene="yfdE"
                     /locus_tag="OO309_000009"
     CDS             complement(11356..12501)
                     /gene="yfdE"
                     /locus_tag="OO309_000009"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR5845323.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(12575..13519)
                     /gene="yfdV"
                     /locus_tag="OO309_000010"
     CDS             complement(12575..13519)
                     /gene="yfdV"
                     /locus_tag="OO309_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR5845324.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(13589..15283)
                     /gene="oxc"
                     /locus_tag="OO309_000011"
     CDS             complement(13589..15283)
                     /gene="oxc"
                     /locus_tag="OO309_000011"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR5845325.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15337..16587)
                     /gene="frc"
                     /locus_tag="OO309_000012"
     CDS             complement(15337..16587)
                     /gene="frc"
                     /locus_tag="OO309_000012"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR5845326.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17100..17723)
                     /locus_tag="OO309_000013"
     CDS             complement(17100..17723)
                     /locus_tag="OO309_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR5845327.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18020..18295
                     /locus_tag="OO309_000014"
     CDS             18020..18295
                     /locus_tag="OO309_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5845328.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18372..18614)
                     /locus_tag="OO309_000015"
     CDS             complement(18372..18614)
                     /locus_tag="OO309_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR5845329.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            18967..19887
                     /gene="lpxP"
                     /locus_tag="OO309_000016"
     CDS             18967..19887
                     /gene="lpxP"
                     /locus_tag="OO309_000016"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR5845330.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20242..20313
                     /gene="ypdK"
                     /locus_tag="OO309_000017"
     CDS             20242..20313
                     /gene="ypdK"
                     /locus_tag="OO309_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR5845331.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20378..21616)
                     /gene="alaC"
                     /locus_tag="OO309_000018"
     CDS             complement(20378..21616)
                     /gene="alaC"
                     /locus_tag="OO309_000018"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR5845332.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            21993..23479
                     /locus_tag="OO309_000019"
                     /pseudo
     CDS             21993..23479
                     /locus_tag="OO309_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            23494..24228
                     /gene="ypdB"
                     /locus_tag="OO309_000020"
     CDS             23494..24228
                     /gene="ypdB"
                     /locus_tag="OO309_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR5845333.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24241..25098
                     /locus_tag="OO309_000021"
                     /pseudo
     CDS             24241..25098
                     /locus_tag="OO309_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(25101..27596)
                     /gene="ptsP"
                     /locus_tag="OO309_000022"
     CDS             complement(25101..27596)
                     /gene="ptsP"
                     /locus_tag="OO309_000022"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5845334.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(27621..28658)
                     /gene="ypdE"
                     /locus_tag="OO309_000023"
     CDS             complement(27621..28658)
                     /gene="ypdE"
                     /locus_tag="OO309_000023"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5845335.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(28658..29741)
                     /gene="ypdF"
                     /locus_tag="OO309_000024"
                     /pseudo
     CDS             complement(28658..29741)
                     /gene="ypdF"
                     /locus_tag="OO309_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(29756..31003)
                     /locus_tag="OO309_000025"
     CDS             complement(29756..31003)
                     /locus_tag="OO309_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR5845336.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31025..31351)
                     /locus_tag="OO309_000026"
     CDS             complement(31025..31351)
                     /locus_tag="OO309_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR5845337.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(31570..32535)
                     /gene="glk"
                     /locus_tag="OO309_000027"
     CDS             complement(31570..32535)
                     /gene="glk"
                     /locus_tag="OO309_000027"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR5845338.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            32739..33995
                     /locus_tag="OO309_000028"
     CDS             32739..33995
                     /locus_tag="OO309_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR5845339.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34110..34436
                     /locus_tag="OO309_000029"
     CDS             34110..34436
                     /locus_tag="OO309_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5845340.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(34577..35815)
                     /locus_tag="OO309_000030"
     CDS             complement(34577..35815)
                     /locus_tag="OO309_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR5845341.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36151..37353
                     /gene="nupC"
                     /locus_tag="OO309_000031"
     CDS             36151..37353
                     /gene="nupC"
                     /locus_tag="OO309_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR5845342.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(37403..39515)
                     /locus_tag="OO309_000032"
                     /pseudo
     CDS             complement(37403..39515)
                     /locus_tag="OO309_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(39723..39798)
                     /locus_tag="OO309_000033"
     tRNA            complement(39723..39798)
                     /locus_tag="OO309_000033"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39763..39765),aa:Ala,seq:ggc)
     gene            complement(39838..39913)
                     /locus_tag="OO309_000034"
     tRNA            complement(39838..39913)
                     /locus_tag="OO309_000034"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39878..39880),aa:Ala,seq:ggc)
     gene            40134..40493
                     /locus_tag="OO309_000035"
     CDS             40134..40493
                     /locus_tag="OO309_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5845343.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            40495..40887
                     /locus_tag="OO309_000036"
     CDS             40495..40887
                     /locus_tag="OO309_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5845344.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(40939..42354)
                     /gene="gltX"
                     /locus_tag="OO309_000037"
     CDS             complement(40939..42354)
                     /gene="gltX"
                     /locus_tag="OO309_000037"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR5845345.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            42613..42688
                     /locus_tag="OO309_000038"
     tRNA            42613..42688
                     /locus_tag="OO309_000038"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42646..42648,aa:Val,seq:tac)
     gene            42733..42808
                     /locus_tag="OO309_000039"
     tRNA            42733..42808
                     /locus_tag="OO309_000039"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42766..42768,aa:Val,seq:tac)
     gene            42813..42888
                     /locus_tag="OO309_000040"
     tRNA            42813..42888
                     /locus_tag="OO309_000040"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42846..42848,aa:Lys,seq:ttt)
     gene            42919..43044
                     /locus_tag="OO309_000041"
     ncRNA           42919..43044
                     /ncRNA_class="other"
                     /locus_tag="OO309_000041"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43164..43505
                     /locus_tag="OO309_000042"
     CDS             43164..43505
                     /locus_tag="OO309_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR5845346.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(43496..44422)
                     /locus_tag="OO309_000043"
     CDS             complement(43496..44422)
                     /locus_tag="OO309_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5845347.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            44512..45510
                     /locus_tag="OO309_000044"
     CDS             44512..45510
                     /locus_tag="OO309_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR5845348.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(45507..45725)
                     /locus_tag="OO309_000045"
     CDS             complement(45507..45725)
                     /locus_tag="OO309_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR5845349.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(45727..47742)
                     /gene="ligA"
                     /locus_tag="OO309_000046"
     CDS             complement(45727..47742)
                     /gene="ligA"
                     /locus_tag="OO309_000046"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR5845350.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(47813..48799)
                     /gene="zipA"
                     /locus_tag="OO309_000047"
     CDS             complement(47813..48799)
                     /gene="zipA"
                     /locus_tag="OO309_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR5845351.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            49029..49790
                     /gene="cysZ"
                     /locus_tag="OO309_000048"
     CDS             49029..49790
                     /gene="cysZ"
                     /locus_tag="OO309_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR5845352.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            49975..50946
                     /gene="cysK"
                     /locus_tag="OO309_000049"
     CDS             49975..50946
                     /gene="cysK"
                     /locus_tag="OO309_000049"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR5845353.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            51330..51587
                     /gene="ptsH"
                     /locus_tag="OO309_000050"
     CDS             51330..51587
                     /gene="ptsH"
                     /locus_tag="OO309_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR5845354.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            51632..53359
                     /gene="ptsI"
                     /locus_tag="OO309_000051"
     CDS             51632..53359
                     /gene="ptsI"
                     /locus_tag="OO309_000051"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR5845355.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            53400..53909
                     /gene="crr"
                     /locus_tag="OO309_000052"
     CDS             53400..53909
                     /gene="crr"
                     /locus_tag="OO309_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR5845356.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(53951..54802)
                     /gene="pdxK"
                     /locus_tag="OO309_000053"
                     /gene_synonym="thiJ"
     CDS             complement(53951..54802)
                     /gene="pdxK"
                     /locus_tag="OO309_000053"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR5845357.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            54907..55281
                     /locus_tag="OO309_000054"
     CDS             54907..55281
                     /locus_tag="OO309_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR5845358.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            55314..56037
                     /locus_tag="OO309_000055"
                     /pseudo
     CDS             55314..56037
                     /locus_tag="OO309_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(56241..57152)
                     /gene="cysM"
                     /locus_tag="OO309_000056"
     CDS             complement(56241..57152)
                     /gene="cysM"
                     /locus_tag="OO309_000056"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR5845359.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(57286..58383)
                     /gene="cysA"
                     /locus_tag="OO309_000057"
     CDS             complement(57286..58383)
                     /gene="cysA"
                     /locus_tag="OO309_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR5845360.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(58373..59248)
                     /gene="cysW"
                     /locus_tag="OO309_000058"
     CDS             complement(58373..59248)
                     /gene="cysW"
                     /locus_tag="OO309_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR5845361.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(59248..60081)
                     /gene="cysT"
                     /locus_tag="OO309_000059"
     CDS             complement(59248..60081)
                     /gene="cysT"
                     /locus_tag="OO309_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR5845362.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60081..61097)
                     /gene="cysP"
                     /locus_tag="OO309_000060"
     CDS             complement(60081..61097)
                     /gene="cysP"
                     /locus_tag="OO309_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR5845363.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(61401..62192)
                     /gene="ucpA"
                     /locus_tag="OO309_000061"
     CDS             complement(61401..62192)
                     /gene="ucpA"
                     /locus_tag="OO309_000061"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR5845364.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(62379..63179)
                     /gene="murR"
                     /locus_tag="OO309_000062"
     CDS             complement(62379..63179)
                     /gene="murR"
                     /locus_tag="OO309_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR5845365.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            63343..64239
                     /gene="murQ"
                     /locus_tag="OO309_000063"
     CDS             63343..64239
                     /gene="murQ"
                     /locus_tag="OO309_000063"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR5845366.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            64243..65667
                     /gene="murP"
                     /locus_tag="OO309_000064"
     CDS             64243..65667
                     /gene="murP"
                     /locus_tag="OO309_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR5845367.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(65847..66746)
                     /gene="yfeX"
                     /locus_tag="OO309_000065"
     CDS             complement(65847..66746)
                     /gene="yfeX"
                     /locus_tag="OO309_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR5845368.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(66842..67417)
                     /locus_tag="OO309_000066"
     CDS             complement(66842..67417)
                     /locus_tag="OO309_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR5845369.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(67478..67927)
                     /locus_tag="OO309_000067"
     CDS             complement(67478..67927)
                     /locus_tag="OO309_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR5845370.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(67914..68339)
                     /locus_tag="OO309_000068"
     CDS             complement(67914..68339)
                     /locus_tag="OO309_000068"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5845371.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            68553..69422
                     /gene="amiA"
                     /locus_tag="OO309_000069"
     CDS             68553..69422
                     /gene="amiA"
                     /locus_tag="OO309_000069"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR5845372.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            69426..70325
                     /gene="hemF"
                     /locus_tag="OO309_000070"
     CDS             69426..70325
                     /gene="hemF"
                     /locus_tag="OO309_000070"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR5845373.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(70331..71383)
                     /gene="eutR"
                     /locus_tag="OO309_000071"
                     /pseudo
     CDS             complement(70331..71383)
                     /gene="eutR"
                     /locus_tag="OO309_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(71429..71929)
                     /gene="eutK"
                     /locus_tag="OO309_000072"
     CDS             complement(71429..71929)
                     /gene="eutK"
                     /locus_tag="OO309_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR5845374.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(71942..72601)
                     /gene="eutL"
                     /locus_tag="OO309_000073"
     CDS             complement(71942..72601)
                     /gene="eutL"
                     /locus_tag="OO309_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR5845375.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            73783..74574
                     /locus_tag="OO309_000074"
     CDS             73783..74574
                     /locus_tag="OO309_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5845376.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            75294..75689
                     /locus_tag="OO309_000075"
     CDS             75294..75689
                     /locus_tag="OO309_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5845377.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            75707..75970
                     /locus_tag="OO309_000076"
     CDS             75707..75970
                     /locus_tag="OO309_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5845378.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            75993..76535
                     /locus_tag="OO309_000077"
     CDS             75993..76535
                     /locus_tag="OO309_000077"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5845379.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            76674..76961
                     /locus_tag="OO309_000078"
     CDS             76674..76961
                     /locus_tag="OO309_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5845380.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            77000..77206
                     /locus_tag="OO309_000079"
     CDS             77000..77206
                     /locus_tag="OO309_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5845381.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            77518..78003
                     /locus_tag="OO309_000080"
     CDS             77518..78003
                     /locus_tag="OO309_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5845382.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            77984..78262
                     /locus_tag="OO309_000081"
     CDS             77984..78262
                     /locus_tag="OO309_000081"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5845383.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            78255..79715
                     /locus_tag="OO309_000082"
     CDS             78255..79715
                     /locus_tag="OO309_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5845384.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            79749..80558
                     /gene="murI"
                     /locus_tag="OO309_000083"
     CDS             79749..80558
                     /gene="murI"
                     /locus_tag="OO309_000083"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5845385.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(80677..81371)
                     /locus_tag="OO309_000084"
     CDS             complement(join(80677..81125,81125..81371))
                     /locus_tag="OO309_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5845386.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(81890..83263)
                     /gene="eutA"
                     /locus_tag="OO309_000085"
     CDS             complement(81890..83263)
                     /gene="eutA"
                     /locus_tag="OO309_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR5845387.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(83238..83717)
                     /gene="eutP"
                     /locus_tag="OO309_000086"
     CDS             complement(83238..83717)
                     /gene="eutP"
                     /locus_tag="OO309_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR5845388.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(83730..84065)
                     /gene="eutS"
                     /locus_tag="OO309_000087"
     CDS             complement(83730..84065)
                     /gene="eutS"
                     /locus_tag="OO309_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR5845389.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(84358..86637)
                     /gene="maeB"
                     /locus_tag="OO309_000088"
     CDS             complement(84358..86637)
                     /gene="maeB"
                     /locus_tag="OO309_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR5845390.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            86926..87876
                     /gene="tal"
                     /locus_tag="OO309_000089"
     CDS             86926..87876
                     /gene="tal"
                     /locus_tag="OO309_000089"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5845391.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            87896..89899
                     /gene="tkt"
                     /locus_tag="OO309_000090"
     CDS             87896..89899
                     /gene="tkt"
                     /locus_tag="OO309_000090"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5845392.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(90002..91045)
                     /locus_tag="OO309_000091"
     CDS             complement(90002..91045)
                     /locus_tag="OO309_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR5845393.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(91171..91746)
                     /gene="nudK"
                     /locus_tag="OO309_000092"
     CDS             complement(91171..91746)
                     /gene="nudK"
                     /locus_tag="OO309_000092"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR5845394.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(91814..93793)
                     /gene="aegA"
                     /locus_tag="OO309_000093"
     CDS             complement(91814..93793)
                     /gene="aegA"
                     /locus_tag="OO309_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR5845395.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            93999..95716
                     /gene="narQ"
                     /locus_tag="OO309_000094"
                     /pseudo
     CDS             93999..95716
                     /gene="narQ"
                     /locus_tag="OO309_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            95918..99031
                     /gene="acrD"
                     /locus_tag="OO309_000095"
     CDS             95918..99031
                     /gene="acrD"
                     /locus_tag="OO309_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR5845396.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(99130..99189)
                     /gene="ypfM"
                     /locus_tag="OO309_000096"
     CDS             complement(99130..99189)
                     /gene="ypfM"
                     /locus_tag="OO309_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR5845397.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            99570..99926
                     /locus_tag="OO309_000097"
     CDS             99570..99926
                     /locus_tag="OO309_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR5845398.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            99930..101057
                     /gene="dapE"
                     /locus_tag="OO309_000098"
     CDS             99930..101057
                     /gene="dapE"
                     /locus_tag="OO309_000098"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR5845399.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            101085..101285
                     /locus_tag="OO309_000099"
     CDS             101085..101285
                     /locus_tag="OO309_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR5845400.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(101395..102093)
                     /gene="ypfH"
                     /locus_tag="OO309_000100"
     CDS             complement(101395..102093)
                     /gene="ypfH"
                     /locus_tag="OO309_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5845401.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(102167..104182)
                     /gene="tmcA"
                     /locus_tag="OO309_000101"
     CDS             complement(102167..104182)
                     /gene="tmcA"
                     /locus_tag="OO309_000101"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR5845402.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(104197..105060)
                     /locus_tag="OO309_000102"
     CDS             complement(104197..105060)
                     /locus_tag="OO309_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR5845403.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(105228..105941)
                     /gene="purC"
                     /locus_tag="OO309_000103"
     CDS             complement(105228..105941)
                     /gene="purC"
                     /locus_tag="OO309_000103"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR5845404.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(106154..107188)
                     /gene="bamC"
                     /locus_tag="OO309_000104"
     CDS             complement(106154..107188)
                     /gene="bamC"
                     /locus_tag="OO309_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR5845405.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(107205..108083)
                     /gene="dapA"
                     /locus_tag="OO309_000105"
     CDS             complement(107205..108083)
                     /gene="dapA"
                     /locus_tag="OO309_000105"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR5845406.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            108229..108801
                     /locus_tag="OO309_000106"
     CDS             108229..108801
                     /locus_tag="OO309_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR5845407.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            108801..109271
                     /gene="bcp"
                     /locus_tag="OO309_000107"
     CDS             108801..109271
                     /gene="bcp"
                     /locus_tag="OO309_000107"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR5845408.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            109524..110141
                     /gene="hyfA"
                     /locus_tag="OO309_000108"
     CDS             109524..110141
                     /gene="hyfA"
                     /locus_tag="OO309_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR5845409.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            110141..112158
                     /gene="hyfB"
                     /locus_tag="OO309_000109"
                     /pseudo
     CDS             110141..112158
                     /gene="hyfB"
                     /locus_tag="OO309_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            112169..113116
                     /gene="hyfC"
                     /locus_tag="OO309_000110"
     CDS             112169..113116
                     /gene="hyfC"
                     /locus_tag="OO309_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR5845410.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            113133..114572
                     /gene="hyfD"
                     /locus_tag="OO309_000111"
     CDS             113133..114572
                     /gene="hyfD"
                     /locus_tag="OO309_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR5845411.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            114584..115234
                     /gene="hyfE"
                     /locus_tag="OO309_000112"
     CDS             114584..115234
                     /gene="hyfE"
                     /locus_tag="OO309_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR5845412.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFATEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            115239..116837
                     /gene="hyfF"
                     /locus_tag="OO309_000113"
     CDS             115239..116837
                     /gene="hyfF"
                     /locus_tag="OO309_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR5845413.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVL
                     MAKPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLP
                     AQRSTWHDFLPSGTASVSEKHSER"
     gene            116827..118541
                     /gene="hyfG"
                     /locus_tag="OO309_000114"
                     /pseudo
     CDS             116827..118541
                     /gene="hyfG"
                     /locus_tag="OO309_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            118551..119096
                     /gene="hyfH"
                     /locus_tag="OO309_000115"
     CDS             118551..119096
                     /gene="hyfH"
                     /locus_tag="OO309_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR5845414.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            119093..119851
                     /gene="hyfI"
                     /locus_tag="OO309_000116"
     CDS             119093..119851
                     /gene="hyfI"
                     /locus_tag="OO309_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR5845415.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            119844..120257
                     /gene="hyfJ"
                     /locus_tag="OO309_000117"
     CDS             119844..120257
                     /gene="hyfJ"
                     /locus_tag="OO309_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR5845416.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            120287..122116
                     /gene="hyfR"
                     /locus_tag="OO309_000118"
     CDS             120287..122116
                     /gene="hyfR"
                     /locus_tag="OO309_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR5845417.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMA"
     gene            122117..122299
                     /locus_tag="OO309_000119"
     CDS             122117..122299
                     /locus_tag="OO309_000119"
                     /inference="COORDINATES: protein motif:HMM:NF014950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HCR5845418.1"
                     /translation="MLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTT
                     LLSRMQRLGISVREVL"
     gene            122321..123193
                     /locus_tag="OO309_000120"
                     /pseudo
     CDS             122321..123193
                     /locus_tag="OO309_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(123287..124348)
                     /locus_tag="OO309_000121"
     CDS             complement(123287..124348)
                     /locus_tag="OO309_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5845419.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            124561..126024
                     /gene="bepA"
                     /locus_tag="OO309_000122"
     CDS             124561..126024
                     /gene="bepA"
                     /locus_tag="OO309_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR5845420.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            126045..126404
                     /gene="arsC"
                     /locus_tag="OO309_000123"
     CDS             126045..126404
                     /gene="arsC"
                     /locus_tag="OO309_000123"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR5845421.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(126537..127283)
                     /gene="hda"
                     /locus_tag="OO309_000124"
     CDS             complement(126537..127283)
                     /gene="hda"
                     /locus_tag="OO309_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR5845422.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(127333..128622)
                     /gene="uraA"
                     /locus_tag="OO309_000125"
     CDS             complement(127333..128622)
                     /gene="uraA"
                     /locus_tag="OO309_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR5845423.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(128708..129334)
                     /gene="upp"
                     /locus_tag="OO309_000126"
     CDS             complement(128708..129334)
                     /gene="upp"
                     /locus_tag="OO309_000126"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR5845424.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            129658..130695
                     /gene="purM"
                     /locus_tag="OO309_000127"
     CDS             129658..130695
                     /gene="purM"
                     /locus_tag="OO309_000127"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR5845425.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            130695..131333
                     /gene="purN"
                     /locus_tag="OO309_000128"
     CDS             130695..131333
                     /gene="purN"
                     /locus_tag="OO309_000128"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR5845426.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            131505..133571
                     /gene="ppk1"
                     /locus_tag="OO309_000129"
     CDS             131505..133571
                     /gene="ppk1"
                     /locus_tag="OO309_000129"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR5845427.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            133576..135117
                     /gene="ppx"
                     /locus_tag="OO309_000130"
     CDS             133576..135117
                     /gene="ppx"
                     /locus_tag="OO309_000130"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR5845428.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(135156..>136028)
                     /gene="pdeF"
                     /locus_tag="OO309_000131"
     CDS             complement(135156..>136028)
                     /gene="pdeF"
                     /locus_tag="OO309_000131"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5845429.1"
                     /translation="LLGELNTVAELSIVTNAPENMQRCGAMYLQRELKDKVAMMNRLQ
                     QALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELIGPGSFLPVAHEFGLSSSIDM
                     WVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEV
                     TESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRN
                     IVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPL
                     IDTLNEIEPIRESA"
BASE COUNT        33936 a        35009 c        33442 g        33641 t
ORIGIN      
        1 aatgacgatg caattcgcga agtacaatgt ctggctacca gtcgggatgg tattcatttc
       61 gagaaacagg gtgtgatcct cactccacca gaaggaatca tgcacttccg cgatcctaaa
      121 gtgtggcgtg aagccgacac atggtggatg gtagtcgggg cgaaagatcc aggcaacacg
      181 gggcagatcc tgctttatcg cggcagttca ttgcgtgaat gggccttcga tcgcgtactg
      241 gcccacgctg atgcgggtga aagctatatg tgggaatgtc cggacttttt cagccttggc
      301 gatcagcatt atctgatgtt ttccccgcag ggaatgaatg ccgagggata cagttaccga
      361 aatcgctttc aaagtggcgt aatacccgga atgtggtcgc caggacgact ttttgcacaa
      421 tccgggcatt ttactgaact tgataacggg catgactttt atgcaccaca aagcttttta
      481 gcgaaggatg gtcggcgtat tgttatcggc tggatggata tgtgggaatc gccaatgccc
      541 tcaaaacgtg aaggatgggc aggctgcatg acgctggcgc gcgagctatc agagagcaat
      601 ggcaaacttc tacaacgccc ggttcacgaa gctgagtcgt tacgccagca gcatcaatct
      661 gtctctcccc gcagaatcag caataaatat gttttgcagg aaaacgcgca agcagttgag
      721 attcagttgc agtgggcgct gaagaacagt gatgccgaac attacggatt acagctcggc
      781 actggaatgc ggctgtatat tgataaccaa tctgagcgac ttattttgtg gcggtattac
      841 ccacacgaga atttagacgg ctaccgtagt attcccctcc cgcagcgtga cacgctcgcc
      901 ctaaggatat ttatcgatac atcatccgtg gaagtattta ttaacgacgg ggaagcggtg
      961 atgagtagtc gaatctatcc gcagccagaa gaacgggaac tgtcgcttta tgcctcccac
     1021 ggagtggctg tgctgcaaca tggagcactc tggctactgg gttaacataa tatcaggtgg
     1081 aacaacggat caacagcggg caagggatcc gcgtcactct tcccccttca cgaccttcaa
     1141 taatatgcaa tgcagcttcc cgcccgataa tgtcatgtgg aagctgaatt gtggtcagcg
     1201 gcggtaaaaa cagatgcccg acgccaacca gattatcaaa gcccattaca gcgacatcct
     1261 gcgggatacg tacccccttc gccaaaagaa cctgataagc cacaaaggct gcgcgatcgt
     1321 taccacatat cagaacatca aaatctggtt tgcccgattt gaagtgagca ttgagtaaac
     1381 ttgcgagatc ggtgtagtga tcatcacctg ttgccatgtg aaattgtttc acctcagcca
     1441 gatctcgtcc agcatcacgc caagcctgct caaatccctg ccgacgatac cctgttgcca
     1501 acgcactttc cggtagccag aagcataatg gttgacgata gcccgccgcg agcaaatgct
     1561 gtgttgattc atattgtgca gtgtaatcat cagggatata actgggtaac gctgggtcat
     1621 ccgccacaca gttcgccaat acaatatttt caccatacag agactcaggc agcgtgatat
     1681 gtcgcagccc cattgtagta tagataatgc catccggacg gtgggcaagc agctgacgtg
     1741 ccgcgcgggc agcgtcatct tcagaaaaaa tattgattaa aaaactattc cagccgaact
     1801 cgctggcggt ttgctcaatg gcaagcagaa tatcaacaga gaaaggagtg gtagccgtgt
     1861 cctgcgccag cacggcgaga gtcgacggct tacgtccttg agcgcgcatc ttacgggcgg
     1921 aaagatcagg aacataattc agggtctgga ttgcctgcaa tacgcggtca cgcgttgcag
     1981 gacgcacaga ttctgcatta tgcatcaccc gggagactgt catcatcgac actcccgcca
     2041 ggcgtgcgac atcctttaat gaagccatac ccaagccgtt tgccgtaaaa cgggcactgt
     2101 agcagaaaca gacgtcactg gcgagatcca acgccctatc acctgacaca gcaatacaat
     2161 aaaaaataac aataattccc ggacaattgt ccccaattcc gcctctgttc tcgccaacga
     2221 gtaaccatat aggcaagtaa gtaaaacatg ccaactggca tcgccaacct ggagaagaaa
     2281 atataaatat ggatcattgt gctgccactg tatcccgaca tcagcccgga aattattgcg
     2341 attgctatcg gttcaggtgc aattggctgc acgatcgtta cggactcgct tttctggctg
     2401 gtgaagcaat attgcggcgc tacgctcaat gaaacattta aatactatac gacagcgaca
     2461 tttatcgctt cagtcgtcgc tctggcgggc acattcctgc tgtcatttat catctaagcg
     2521 caaagagacg tactatggaa aacgctaaaa tgaactcgct catcgcccag tatccgttgg
     2581 taaaggatct ggttgctctt aaagaaacca cctggtttaa tcctagcacg acctcattgg
     2641 ctgaaggttt accttatgtt ggcctgaccg aacaggatgt tcaggacgcc catgcgcgct
     2701 tatcccgttt tgcaccctat ctggcaaaag catttgctga aacagctgcc actgggggga
     2761 ttattgaatc agaactggtt gccattccgg ctatgcaaaa acggctggaa aaggaatatc
     2821 aacaaccgat cagcgggcaa ctcctactga aaaaagatag ccatttgccc atttccggct
     2881 ctataaaagc acgcggcggg atttatgaag tcctggcaca tgcagaaaaa ctggctctgg
     2941 aagcggggtt gctgacgctt gatgatgact acagcaaact gctttctccg gagtttaaac
     3001 agttctttag ccaatacagc attgctgtgg gctcaaccgg aaatctgggg ttatcaatcg
     3061 gcattatgag cgcccgcatt ggctttaagg tgacagttca tatgtctgct gatgcccggg
     3121 catggaaaaa agcgaaactg cgcagccatg gcgttacggt cgtggaatat gagcaagatt
     3181 atggtgttgc cgtcgaggaa ggacgtaaag cagcgcagtc tgacccgaac tgtttcttta
     3241 ttgatgacga aaattcccgc acgttgttcc ttgggtattc cgttgctggc cagcgtctta
     3301 aagcgcaatt tgcccagcaa ggccgtatcg tcgatgctga taaccctctg tttgtctatc
     3361 tgccgtgtgg tgttggcggt ggtcctggtg gcgtcgcatt cgggcttaaa ctggcgtttg
     3421 gcgatcatgt tcactgcttt tttgccgaac caacgcactc cccttgtatg ttgttaggcg
     3481 tccatacagg attacacgat cagatttctg ttcaggatat tggtatcgac aaccttaccg
     3541 cagcggatgg ccttgcagtt ggtcgcgcat catgctttgt cgggcgggca atggagcgtc
     3601 tgctggatgg cttctatacc cttagcgatc aaaccatgta tgacatgctt ggctggctgg
     3661 cgcaggaaga aggtattcgt cttgaacctt cagcactggc gggtatggcc ggacctcagc
     3721 gcgtgtgtgc atcagtaagt taccaacaga tgcacggttt cagcgcagaa caattgcgta
     3781 atgccactca tctggtgtgg gcgacgggag gtggaatggt gccggaagaa gagatgaatc
     3841 aatatctggc aaaaggccgt taataacgtt tcaacgcaac atcgcaatcc tttccctggg
     3901 tgagcgatgc tgccgatggc gcagacttaa gatccccggt cttacccgct ataaccccct
     3961 ccttttatca tcacccaacg cctttcgctg taaacggcgg tttcgcaaac caaaccaaaa
     4021 ccgtaagtaa gataaaagca atagccgcca tacggaaaat ctcatttgca gaaatagaaa
     4081 gtgactgctg ggttatttca ttattaattt cattaagaac tcctgaaagc gaaccatagt
     4141 atttatccat aatttgtgac gatgaattaa acacggggtt aaattgatcg atggttgctg
     4201 tcaactgact atggtgtaac gattcgcgtc gtccccacag cgtcattgtc aacgacgtac
     4261 caactgatcc tgacaaggta cgaaaaaaat tactcatact cgaggcattg gcaaatttat
     4321 tatctggcaa gcctgaaaac gaaatcgttg ttaagggtaa aaagaaacag gcaacggcga
     4381 atccctggaa aaactgtggc aaaatgatgc ctgtaaaatc aatcgttggc ataaatgtca
     4441 cagaacgcca gtaatagcaa accgcataca tcaaaaaact gaatgtcact aacacccgca
     4501 tgtctatttt attgccataa cgtcctatca aaggtgaaat caatagtggc atgatgccga
     4561 tgggcgcata agcaagcccg gcccatatcg cattataccc catcgttttc tggagtaact
     4621 gcggcataag gacgatcgct ccagagtaaa ataaatacgc gcaggtgata ctcacaatac
     4681 caatggtgaa attacgggac ttaaacaaac tgagatcaag aatcgggttc tctgaggtcg
     4741 attcccaaat gactaaagag atcagagaaa taactgatac tactgttaat attattattg
     4801 tactcgagtt gaaccaatcc agatcgcgcc ctttatcaag cataatttgc aagccaccaa
     4861 caccgagcac taacagggtc agtcttggta gattcatttt taccggtgaa gtttcagttt
     4921 ctcttccttt aagtaaggtt aagcataatg tcaggacgat aatccccata gggacattga
     4981 ttaaaaatat ccaaccccag ctaaagttat cgcaaatata gccgcccaat atcggcccac
     5041 atatcggagc gataatcacg gtcattgacc ataatgccag agcaaatgtt cttttttctg
     5101 gtggataatt ccttaataat aaactctgtg acagtggaat taacggcccc gccattaatc
     5161 cctgaacgac tctaaaaaat atcagcacat caagattggt cgataaacta cacattaagg
     5221 aagacagcga aaaaaaagtg actgaaagta aaaataacct taattcgcct attctttgtg
     5281 ccagcctgcc ggtaacaggg atcgcaatgg catttgctac gccaaacgag gtgataaccc
     5341 aggtgccttc gtctgttgat gctcccagaa agccagatat tgtcggtatt gcgacgttag
     5401 aaatagtgga atccaacatt tgcataaacg tcgctaatga caatgcaata gtaacgcacc
     5461 ataacgtccc accggttaat ggtgccggag ttgatttagt gattgccatt aaagttgtcc
     5521 attatgcgaa ataatgttgc taatttcttt ttctatcgga ctggtatcga taactaaagc
     5581 cttactggta taagcaggca tggaggtcac ggttgaagcc agatcaggca tctcagcaat
     5641 gtcttcgtcc ttcgtatcaa tagttgcagt catcgataaa ccaatgcgca aggggtgttc
     5701 catgagttct tttggatcaa gagaaacttc aaccggtaca cgctgaacga ttttgatcca
     5761 gttccctgtc gcattttgtg caggtaataa ggagaacgca ttgccggttc ccatattgat
     5821 ccctgtcacc cgaccatgaa acacaacatt ttcaccataa agatcgctga taatattgac
     5881 tgattgacca atccgtacat ccgtgagttg tgtttcttta aagttggcat taacccacat
     5941 ttgacgtgcc ggtacgacag ccattaacga ttgtccggaa ctcactgttt cgccgacctg
     6001 aacacttcgc tgggcaatat agccagtaac cggactcctt atatccgtac gtttaagcac
     6061 caaccaggct tctttggttg catccgccgc ttcaactact tgtggctgac ggtttaatgg
     6121 tgtgttcatt actaaagctt tattcgcttt ataagcctgg atagcggcat tcagtgccgc
     6181 tttgctactt attaacgtat ctttggtatg ctccagcgtt tcttttgaaa taaccccctg
     6241 cttcgctaaa ggcacacgac ggttataatc ttctaaagat tgttgatact gaatacgtgc
     6301 tgaagcgact tcggcactgt attgtttatc ctgtaagtac agtttattcg tttgccgaac
     6361 aatatttgcc agattatttt tagctttatt gagtgcgata gtggcatcag ttttatccag
     6421 tgaaactaaa atgtcaccct gtcgaacgta gttcgtatct ttatgattaa ctacagtgac
     6481 actacctgag acttgtgcag aaattggatc tgcattcccc gtgacatagg cgtcatctgt
     6541 actaatcatg tcttctaatt ccattgacca ataggcatag gcacctgaaa acgcaataaa
     6601 taaaactatc accaataaag aaaagtattt ttttctgtta gactgttttt tatttgaatt
     6661 aatctgttcc actattatct ctcatttcgc atagatgaaa tttatgaatt gaatgaaaag
     6721 agtattcatt cttcatatca aacacagtgc atgtgttgca cattatccca ggaacgttgg
     6781 aggcctaatt taaatgaggt gcaaaaaaaa caacagagga ctaaaccgtg gcttttgcaa
     6841 tacaattctt acgcctgtag gattagtgag aagacttata gtgctaactt gaaactataa
     6901 atcatcggta taatccctga ttttattgtt gacatttcat ttatgccgac tatttatatg
     6961 gtatacttgt cgaattatct taaaggaaac tcagattttc ttatttttat tgagaaaatg
     7021 agatgacgcc ttatgtctgt attactacag ggagaaggga gatgcttcat tgcaaaggga
     7081 ataatctatg aacgcaataa ttattgatga ccatcctctt gctatcgcag caattcgtaa
     7141 tttattgatc aaaaacgata ttgaaatctt agcggagtta actgaaggcg gaagcgccgt
     7201 tcagcgggtg gaaacactta agcctgatat cgtcatcatt gatgtcgata tccccggagt
     7261 taacggtatc caggtgttag aaacgctgag gaagcgccaa tatagcggaa ttattattat
     7321 cgtctccgct aaaaatgacc atttttacgg gaaacattgt gctgatgctg gcgctaatgg
     7381 tttcgtgagt aaaaaagaag gcatgaacaa tatcattgcg gctattgaag ctgcaaaaaa
     7441 tggctactgc tatttcccct tctctctcaa ccggtttgtt ggaagtttaa cgtccgacca
     7501 gcaaaaactc gactccttat cgaaacaaga aattagtgtc atgcggtata ttcttgatgg
     7561 caaggataat aatgacattg cggaaaaaat gttcatcagc aacaaaactg tcagcacgta
     7621 taaaagtcgc ctgatggaaa aattagaatg taaatcactg atggatcttt acacattcgc
     7681 gcaacgtaac aaaatcggct aatcacatga agtttttacc ctattttttt cttctctgtt
     7741 gtggtctttg gtcgaccata agtttcgcag acgaagatta catcgaatat cgtggcatca
     7801 gtagtaacaa ccgtgtcaca cttgatccag tacgtctgag caacaaggaa ttacgttggt
     7861 tagcgagcaa aaaaaatctt gtgattgcag tacacaagtc ccaaacggct accttgttgc
     7921 ataccgattc gcagcaacag gttcgtggta ttaatgctga ttatttaaat cttttaaaaa
     7981 gagcgttaaa tatcaaatta acactccggg aatacgcaga tcatcaaaaa gcaatggacg
     8041 cgcttgcaga aggtgaagtc gatatagtgt tatcacattt agttacttcg ccgcctctta
     8101 ataatgacat tgctgcaacc aaaccattga taattacctt tccggcgctg gtaaccaccc
     8161 ttcacgactc aatgcgaccg cttacctcac caaaaccagt aaatattgct cgggtagcaa
     8221 attacccccc agacgaggta attcatcaat catttccaaa agcaacaatt atctctttta
     8281 caaatttata tcaggcatta gcatccgtct cagctgggca caatgattac tttattggta
     8341 gtaacatcat taccagcagt atgatttccc gctatttcac tcactcctta aatgtagtga
     8401 aatattataa ctcgccgcgt caatataatt ttttcttgac cagaaaagaa tctgtcattc
     8461 ttaatgaagt actcaataga tttgttgatg ctttaacaaa tgaagttcgc tatgaagtat
     8521 cacaaaattg gcttgataca ggaaacctgg cctttctgaa caaaccatta gaactcactg
     8581 aacatgaaaa acagtggatt aagcagcatc ccaatttaaa ggtgctggaa aatccttact
     8641 cgccccccta ttctatgacg gatgaaaatg gctcggttcg gggcgttatg ggggacattc
     8701 ttaatattat taccttgcaa acaggtttaa atttttctcc gatcaccgtt tcacacaata
     8761 tccatgctgg aacacaactt agccccggag gatgggatat aatacctggc gctatttata
     8821 gtgaagatcg agaaaataat gttttatttg ctgaagcctt cataacaacg ccttacgttt
     8881 ttgtcatgca aaaagcgcct gacagtgaac aaacattaaa aaaaggaatg aaagttgcca
     8941 ttccatatta ttatgagctg cattcgcaat taaaagagat gtatccggag gttgaatgga
     9001 tacaggtcga taatgccagc gctgcatttc acaagattaa ggaaggtgaa cttgatgctc
     9061 tggtcgcgac acagctaaat tcgcgttaca tgatcgatca ttactatcct aatgaacttt
     9121 atcattttct tattcctggc gttccgaatg catcgctttc gttcgctttt cctcgcggag
     9181 aaccggaact taaggatatt attaataaag cactgaatgc aattccccca agcgaagttc
     9241 tgcgcctgac ggaaaaatgg attaaaatgc ccaatgtgac cattgacaca tgggacctat
     9301 atagcgagca attttatatt gttacgacat tatccgtttt attagttggc agtagccttt
     9361 tatggggatt ctacctgtta cgctcagttc gtcgtcgtaa agtcattcag ggtgatttag
     9421 aaaaccaaat atcattccga aaagcactct cggattcctt accgaatcca acttatgttg
     9481 taaactggca aggtaatgtc attagtcata atagtgcttt tgaacattat ttcactgcgg
     9541 attactacaa aaatgcaatg ttaccattag aaaacagtga ttcacccttt aaagatgttt
     9601 tttctaatgc gcatgaagtc acagcagaaa cgaaagaaaa tcgaacaata tacacacagg
     9661 tatttgaaat tgataatggc atcgagaaaa gatgcattaa tcactggcat acattatgca
     9721 atcttcctgc aagtgacaat gcagtatata tttgtggttg gcaagatatt actgaaacgc
     9781 gtgatctaat taatgcactc gaggtagaaa aaaataaagc gataaaggct accgtagcaa
     9841 aaagtcagtt tctggcaacg atgagtcacg aaataagaac accaataagc tctattatgg
     9901 gcttcctgga acttctgtcg ggttctggtc ttagcaagga gcaacgggtg gaggcgattt
     9961 cacttgccta cgccaccgga caatcactcc tcggcttaat tggtgaaatc cttgatgtcg
    10021 acaaaattga atcgggtaac tatcaacttc aaccacaatg ggtcgatatc cctactttag
    10081 tccagaacac ttgtcactct ttcggtgcga ttgctgcaag caaatcgatc gcattaagtt
    10141 gcagcagtac atttcctgat cattacctgg ttaagatcga ccctcaggcg tttaagcagg
    10201 tcttatcaaa tttactgagt aatgctctca aatttaccac cgagggggca gtaaaaatta
    10261 cgacctccct gggtcacatt gatgacaacc acgctgttat caaaatgacg attatggatt
    10321 ctggaagtgg attatcgcag gaagaacaac aacaactgtt taagcgctac agccaaacaa
    10381 gtgcaggtcg tcagcaaaca ggttctggtt taggcttaat gatctgcaaa gaattaatta
    10441 aaaatatgca gggcgattta tcattagaaa gtcatccagg cataggaaca acatttacga
    10501 tcacaatccc ggtagaaatt agccagcaag tggcgactgt cgaggcaaaa gcagaacaac
    10561 ccatcacact acctgaaaag ttgagcatat taatcgcgga tgatcatccg accaacaggc
    10621 tattactcaa acgccagcta aatctattag gatatgatgt tgatgaagcc actgatggtg
    10681 tgcaagcgct acacaaagtc agtatgcaac attatgatct gcttattact gacgttaata
    10741 tgccgaatat ggatggtttt gagttgactc gcaaactccg tgagcaaaat tcttccttac
    10801 ccatctgggg gcttacagcc aacgcacagg ctaacgaacg tgaaaaaggg ttaagttgcg
    10861 gcatgaactt atgtttgttc aaaccgttga ccctggatgt actgaaaaca catttaagtc
    10921 agttacacca ggttgcgcat attgcacctc agtatcgcca ccttgatatc gaagccctga
    10981 aaaataatac ggcgaatgat ctacaactga tgcaggagat tctcatgact ttccagcatg
    11041 aaacgcataa agatctaccc gctgcgtttc atgcactaga agctggcgat aacagaactt
    11101 tccatcagtg tattcatcgc atccacggtg cggctaacat cctgaatttg caaaagttga
    11161 ttaatattag ccatcagtta gaaataacac ctgcttcaga tgacagtaag cctgaaattc
    11221 ttcagctgct gaactctgtt aaagaacaca ttgcagagct ggaccaggag attgctgttt
    11281 tctgtcagaa aaatgactaa attgcggctc ccacaatgtt ctaatgtggg agctatttac
    11341 cagcacatct tacatttatg atgagaactc ctggcgaatt tgttccccat gctggtcgag
    11401 cgttgccgct cccggcataa catgcgggtc cgcgcagccg ctgattttta tcggattacc
    11461 cggcatcatt attcctccgg cttcaatcaa catatttctc gcctgagttt gtggcaaatt
    11521 aatggcctca gccacactta ataacggcgc gacgggcaca ccaacttcat gtattttggc
    11581 taaccaaact tcagctgcct gcgtttttaa cgttcgctca atatattgtt taagaatagc
    11641 ctggttttgt acgcgtaaaa tattgctgct aaatcgggga tcattaacca gttccgtaag
    11701 ctccagtgcc tggcataacg cagaaaaaag cttgtcatta ccacaacaaa tcgtaatcgg
    11761 cttatcctga gtatcgaaaa catcaaaagg tgccatgtag ggatggcgat ttcccaggcg
    11821 ttgtggtgac ttccctgtcg caatatatgc catcagacca tgctccagaa aactcagcgt
    11881 ggcatcaaac atcgctatat cgacatgcgc ccctctctgg ctcttttcgc ggccataaag
    11941 tgcactcact attccgctga ataaatagac accgccgcat agatccgcaa gagaggtacc
    12001 aacacgcact ggcggagcat cagggtaccc tgtttccatc attatcccac tcattgcctg
    12061 aatgatggta tcgtaggcag gagcatcttt tagcggaccg gtatgtccga aacctgacga
    12121 tgaagcatat atgaggcgcg ggttgatttc ttgtaacctt tcccatgaaa accccagttt
    12181 ttccattgta cctaggcgaa aattctcagc taatacatca gcttgtttga gcatatttat
    12241 aaatatactt ttatcgtgat cattctttaa atcaagaacc acactctctt tgccatgatt
    12301 aataaaactg taatagagtg actgtccatc cacataggga ccaaatgtgc gggtatcatc
    12361 accatgacct ggtggctcaa ctttaattac ccttgcgccc atattataaa gaagttgagt
    12421 tccgaaaggt ccattaagga catgtgtcat atcgataact aataagcctt caaacggccc
    12481 tttgctttca ttatttgtca tttgcttatg ccttgaaaaa agccgaaggt gaaaagacac
    12541 ttcggcattt atgtaaaatc aataacatga ttaattatga aaccagtcga ctgacataaa
    12601 tccacaaggg agccgtgacg acaaagccca gtacgctcac agccaatgac gctgtaccag
    12661 tacgggtgta gacattaaac cggctggcaa taatgattcc ggagaatgcc ggcggtaatg
    12721 cgcctgccag taccatcatc tgcaaatgtt cgctgttcaa atgacatgcc ataccaacga
    12781 gaagcagcgc cagtggcatc agaatcaact tcaggaaggt gttataagca atttcggcac
    12841 tgaactcgaa tttatgtgca gccagagtca accccgcagc gaataccgct acccctgagt
    12901 tagccttcgc aatcagatta aaggttgggt cccatgctgc cggaattttt accccaacca
    12961 gcaccaggat cgttgccaga acaggtgccc atactactgg ctcctttgcc gcagaaatta
    13021 atgcgctcag attactattc ttcttaccat ccgctcctga ggaaggattc agcaaataca
    13081 gaccaatagg aatagtaatt gcgttaacaa taatagaaat aattgctacc actaaacctg
    13141 ttgatacgga atcaccataa ataggatcga gaactgcaaa ccccaggaat ccaatggtag
    13201 gtgaacccgc aattaatgca catacagctg cttctgcatg ggtacgttta aaaaatttgt
    13261 agcaaccaaa ccaggagaaa aagaaacatc cgacaataac cacaagtgat acaagggtca
    13321 gacgagtgtc cgcaaaaatc atttccctgt ttgcccgggt aatagaaaca aatagagccg
    13381 caggaagagc gtagtttaat accagtttat taaatgcgcg ggcttgatct tctgaaaaag
    13441 tttctcgtct gccgctaaaa taacccaaca gcatgattac gataataggc aataaatcgc
    13501 caataaaaaa tgttagcata atagtatttc cgataagggt cccctcacca tcgcataatg
    13561 agttggcaag aggagacgat gtcagtattt aattaccagc gacttgtttt gggttaagtt
    13621 tggtaatatg gccactttca gttcctgctg cagggtcgat aaccacatta ataatggtcg
    13681 gtttgcgcga ctgaataccg gtggttaaag catgacgaag ttcatctgtc gtggtgacgt
    13741 tatagccaac gccacgaaac gcatccatta atttgtcata ccttgcatgg tgcaacagat
    13801 ccgttggtga tggtgcacca gcgccactga gatcaacacc gtctcctctg tagatgccgc
    13861 cattattaaa aataacgatc gtcaccggca ggttatatcg acaaatcgtt tcaatttcca
    13921 tcccactgaa accaaaagca ctatcacctt caatggcgac aaccggagaa ccagaagtca
    13981 cgctagcacc gatggcatag cccataccga tgcccatgac accccaggta ccacaatcca
    14041 gacgacgacg tggtttatac atatcaataa tatttcgtgc attatccagg gtatttgcac
    14101 cttcattaac taaataaata tcctggttct cgcgcaatac gtcgcgcaca gcacttaatg
    14161 cattaaagta atttaatggt tgagtatctg tacttaattt ttcatgcatt ttttgtgcat
    14221 tttgctgctt gtggatattt aaaatatcgc gccataccag tggagtcgta aatgtgtttt
    14281 gtttcagttc tgccagcata ccttgcatac tggatgcaat atcaccaacg actggcacag
    14341 caatggggcg gttgctgtca atttcctgcg gttcaatatc cagttgaata aactgtgtat
    14401 ctgccgccca tcctttttta ccgtgtgcca ataaccaatt cagtcgtgca ccaacaagca
    14461 tgacaacgtc agcatttgcc agggcaaacg aacgcgcagc tgccgcagaa agtggatgcg
    14521 tatcttcaag gatccctttc gccatagaca ttggcaggaa tggaatctga gcactttcaa
    14581 taaattcacg aagctgttca tcagcttgtg aatacgccgc gcctttgcca aggataatta
    14641 atggccgttc agcttttgct aaaagcgaaa ttgcgctagt gactgacttc gggcatggca
    14701 ataatgctgg cgacggattt tcaactttaa caatcgtggt taacgcttcg tctttttcca
    14761 tcgtcgcggc caggacattt gctggcaaat caagataaac tccgccaggg cgacccgata
    14821 cagagacccg gatagcgcgt gccaatgcaa tgccaagatc ctgcggctga ttaacgcgaa
    14881 atgctgcttt ggcatacggt tttgccgcat tcatttggtc cagctcttca taatcacctt
    14941 gctgtaggtc gacgatcgcg cggtcgctgg agccgctaat catgataatc ggaaaaccat
    15001 ttaccgttgc gttggccaat gcggtcaaac cattgaggaa tcctggcgca gaaactgtca
    15061 ggcagatccc cggtttttgg gtaagaaaac cgcttgccgc agcggcatag cctgccgact
    15121 gctcatgacg aaaaccaata taacgaatgc cttccgcctg ggcatggcgt gccatatccg
    15181 tcacaggaat acctacaaca ccataaatag tgtcaatatt attctgtttt aatgcttcaa
    15241 cgatgatatg cataccatct gtcatttgaa gttgatctga catgattaat aacctcgaaa
    15301 tttttataaa ttgggagcca gaagcccccg ttaatatcag atggcgtggt tttgcttcat
    15361 tgcagcaatt tcatcgtcgc tataacccag ctcctgcaat acagcagcgg tatgttcacc
    15421 taatagcggc gcagctttaa tatccggcgt aaaggcagag aatttcattg gacaaccaac
    15481 ggtcagatat tttccacgca acggttgttc cacttcaaca acactgccac tttggcgcaa
    15541 agagggatca agtgaaattt ctttcatact taaaaccggt gcacaaggaa tatcaaactg
    15601 agtcaaatag gccaccgctt catgtttatc aatagtgaca gtgtattttt cgatttcagc
    15661 aaaaatatcg aaaatatgtg gctgtcgggc atgggctgta ctgtatgccg gatcggtaat
    15721 ccattctggt ttgccgatgg ctttacaggt gttttcccag ttttgctcct gaatagtgaa
    15781 ataaatatag gcgttaggat cggtttccca gcctttacat ttcaggatcc agccaggctg
    15841 accgccacca cctgcattac caccgcgggg aactgcatca ccaaatgtac cattcggata
    15901 ctgcgggtat tcttccagat aacccaattt atcgagacgc tgctggtcac gtaatttcac
    15961 gcggcaaagg ttcaatacgg catcctgcat tgacatggtg actcgttgcc cacgccccgt
    16021 tttttcgcga tgcagcaaag cagcaagtaa accgatcagc aaatgcattc cggtgttgct
    16081 gtcacccaac gctgcagcgc ttaccagcgg cggaccatcc caaaaacccg tagtggatgc
    16141 cgcgccaccc gctgcctgag caacgttttc ataggctttt acattcacat aaggcgaaca
    16201 ctcatcaaac cctttgatcg aaccaaaaat cagacgtgga ttgatttctt gaatatgctc
    16261 ccaggtgaag cccatgtgat caatggcccc tggatgaaag ttctcgacta agatatcagc
    16321 ttcgcggatc agcttttcca ttacctcttt gccttccgct gttttggtat ttaactcaat
    16381 agaacgtttg ttactgttaa gcatggtgaa gtaaagcgca tcgatatcag gaatatctcg
    16441 cagttggtga cgcgttacgt caccaacgcc gggacgttca attttaataa cgtcagcgcc
    16501 aaaccaggcc agcatttgag tacaagatgg gccagattgc acaccggtga aatcgagaac
    16561 tttaattcct tgaagtggag ttgacatagc aatacctatt agttaattaa atatgtgtta
    16621 atttagcgtt tatttaaggc ataacgccct ctataaataa tctgcaaaat atcttcgtag
    16681 cggcactcga tatttttaaa ttcctgtatc aagtactgaa aattatttta tcgccacttc
    16741 caattccttt ctgcatggta agtagtgagg acgaaaagaa gaatatgata aatattaatt
    16801 cgtggtaatc agagagagtc gcgacatttg tgatgttatt cttacaactg taagaagcgc
    16861 cggtgattta caagtaagag tatgattacg ctttttaaag cgtaccataa agcataacat
    16921 aataaatgat ttgtgatgac acgggattat tgcggtttac tttgaggaaa ataaaatgcg
    16981 tgtttataga accgcgagcg ccaaataatt ttatgaccac aacctgtcgt attgtggtca
    17041 taaaaaattg tcggctggag ggagttaaat cctttacctc aatatactgc ctgtcagcat
    17101 tagttcacaa acagtgcttc gctatcgacg atgattccat cttccgcacc tttaagcgcc
    17161 aggtttgcct cgtagtattg ctttttatca agcagtttct gcgcatcagc aagtgcatta
    17221 cgagtctgtt taagcggcat cagatattgg ttttccatta cgccaacacc ggccaggcga
    17281 agctcttcca ttgcgccctt tttatcccct ttcgccattt tttcattggc aatttttatc
    17341 gcggcttctt tttcgggtgt tgctacataa ctctcagaaa ttccgacaga tgcattaatg
    17401 acaatatatt ggtcatcatt gagattggtc tttttacctg gcttagcgaa ctttgcccat
    17461 tcggtactgt catctgacag caaagcggaa gcttcattgg tcagttcttt tgccttttcc
    17521 ggatcgccat gaaacagagc caggcgcgct acctgcacgt cacgcatcgc atataaacct
    17581 tgttcagaga tacgctctgc tgctgccgtt ttctgggttt gctgagtttg ctgttgtgcc
    17641 gctaccggtg cattatcagc agcccataca gttgaagatg ccagaattgc tgtgaccatc
    17701 gtagccataa ttaaacgttt catacttacc tcattcttta acagttttag aaaacgccgc
    17761 catcatattg ggtgagcatg aaatgagaaa tcagagagca acggtcattt tgaaagggtt
    17821 atcttacaat tgtagttatt atcgcaaagc ggcatttagt cttacagttg taggaatatg
    17881 cctcctgtcg cagagaacta ttcttatata ccacaaagag atgtcattta ttatattgat
    17941 gtaaatattg attaattata tgtaaataca gccataacat cgtatggttc tcagtgaacc
    18001 attttcagga taactttcta tgaaagtaaa cttaatactt ttcagcttat ttttattggt
    18061 ctctattatg gcatgcaatg tttttgcatt ttccatttct ggtggtgtaa gtgagaggag
    18121 ctataaagag actgaaaaaa catcagcgat gacgaccaca cactctacaa aacttcagcc
    18181 atcacaggcg attttgttta agatgagaga agatgtgcca ccattaaacc tcacagaaga
    18241 aatcacgcct acttatccga caaaggcgaa ttatcttatt catcctgtgc gatagcgtct
    18301 gtcacatggc aggtgaacaa tcatgtctga tgctgatgtg aacacctgcc caaatgagcc
    18361 atcctgaccc actatcgcct gccatacccc accataaagc gaatcatcaa gccacaagcc
    18421 ggggcaagaa acataaatat ccccgtcacc agttcattac cgccagttaa ccacatggtg
    18481 aagtaataaa tgagtgcagc gaggatcacc atgccgaaga aagaagagac cgccgatacc
    18541 acgttcagta cctgaccgat gtagccgctt acgcagacaa tacacagggc gagaaacatc
    18601 cataaataaa tcatagctca acctcctgcc gcgctaaaaa acctgtaact tcagtcactt
    18661 taatagtaag acaatcctta acctctggca atttttgcta agcatcaatg aaaacagcac
    18721 gttaaatgca acgctattgc gtgcggaaaa ttttttactt tgcacgatta ataatcagta
    18781 gctgaaagca gtcagccagt gataaagtag agccaccgca ttcatgctcc tcgtggttat
    18841 gtcctgacta gtctttacac tctttacagg aaccattgtc gtacatgatg gcccaaccaa
    18901 ttgagtgtac cgctgtcgac atggaatctt ctgcgataca acaattcgta tctacagaag
    18961 gtaactatgt ttccacaatg caaattttcc cgcgagtttc tacatcctcg ctactggctc
    19021 acatggtttg ggcttggtgt actctggctt tgggtacagc ttccttatcc tgttctctgc
    19081 tttctcggca cgcgtattgg cgcaatggcg cgaccattcc tgaaacgtcg tgaatctatc
    19141 gcccgtaaaa acctggaact ttgtttcccg cagcattctg cggaagaacg cgagaagatg
    19201 attgccgaaa actttcgttc actcggcatg gcgctggtag aaaccggcat ggcatggttc
    19261 tggcccgaca gtcgcgtacg taaatggttt gatgttgaag ggttggataa ccttaaacgc
    19321 gcacaaatgc aaaatcgcgg cgtaatggtt gtcggcgtcc attttatgtc gctggaactg
    19381 ggcggccgcg tgatgggact gtgccaacca atgatggcta cctatcgtcc acataataat
    19441 cagctgatgg aatgggtgca gacccgtggg cgcatgcgct ctaacaaagc gatgatcggc
    19501 agaaataatc tgcgcggcat tgtcggtgca ctgaagaaag gtgaagcggt atggtttgct
    19561 cccgatcagg attatggtcg taaaggcagc tccttcgcgc cgttctttgc ggtggaaaat
    19621 gtcgccacaa ccaatggcac ctatgttctc tcccgtctct ctggcgcagc catgttgacc
    19681 gtaacgatgg taagaaaagc ggattacagc ggatatcgtt tgttcatcac cccagagatg
    19741 gaaggctacc cgacagatga aaatcaagcc gctgcttata tgaacaagat tatcgagaaa
    19801 gagatcatgc gcgcaccgga gcagtacctc tggatccacc gtcgctttaa aacgcgcccg
    19861 gtgggagaat cgtcgttgta catttaacaa actgctctta ctctttccct cctcaaatcg
    19921 gccataataa cctcatgatt tttatagctt ttttcttaag ttttttcctt cgctgaatat
    19981 ctaattatta ccgtcagaaa ttaaactgtc gccgatatga gacacttgta agttactaaa
    20041 agtaaataag aaaatgcctc ttgtctcgtt tcagttttag gcgtacatta gcgccgtctc
    20101 gagcagttta gctcagaatt ctggggtgat gcccacggta aaatacaggg cttttaaggt
    20161 cgctttgccc ggtgtcaact cactgtatca ggtgtaatga agtcattcag gcgtaacagt
    20221 aattacgcgg agagatgtaa agtgaaatat ttctttatgg gcatttcttt tatggtcatc
    20281 gtttgggccg gtacttttgc cctgatgatc taaaagcaaa ctgtgcaatg aaaaacagga
    20341 gccatcaggc tcctgtttgc atttctgacg acggtgttta ttccgcgttt tcgtgaatat
    20401 gtttgctgct gacgggtagt aaaccgtcgg cacggaacat cgctttaatc cctctgatgg
    20461 cctgacgaat acggtcgtgg ttttcaatca gtgcaaagcg aacatgcgta tcaccatagt
    20521 cgccaaagcc aatccccggc gagacacaaa ccttcgcttc gttaaggagc ttcttggcaa
    20581 attccagcga tcccatggcc gcatatggtt ccgggatttt cgcccagaca tacatcgaag
    20641 ccttcggcat ttcgaccatc cagcccgctt catgcagccc tttaaccaat acatcacgac
    20701 ggcgtttgta ctgttcggca atgtcgcgca cgcactgttg atcgccctcg agtgccgcaa
    20761 tcgccgccac ctgcaacggc gtaaaagtgc cgtaatcgtg atagcttttg attcgcgcca
    20821 gggcactgac cagcgttttg ttgccgacca taaagccgat acgccagccc gccatgttgt
    20881 agcttttcga cagcgtaaag aactcgaccg ccacatcgcg tgcacccggc acctgcataa
    20941 ttgacggcgc tttccagcca tcgtagacga tatcggcata ggccaggtca tggaccacca
    21001 gcacatcgta gcatttcgcc agcgccacaa ccttttcgaa gaactccagt tccacgcatt
    21061 gcgcggttgg gttcgacggg aagccgagga tcatcatctt cggtttcgga taactttcac
    21121 gaatggcgcg ctccagttcg ttgaagaaat cgacaccttc caccagcggc actgagcgta
    21181 cctgcgcccc ggcaatcacc gcgccataaa tatggatcgg gtaacttgga ttcggcacca
    21241 gcaccgtgtc accatgatcc agcgtcgcca gcatcagatg cgccaggccc tctttcgaac
    21301 caatagtgac gatggcttct gattccgggt cgatttcaac gtcgtagcga tcctgatacc
    21361 agcgggaaat ggcgcgacgt aaccgcggaa tgccgcgtga agtggagtaa ccatgcgtgt
    21421 ccgggcgctg ggccacagta cataattttt cgacgatatg cggcggagtc gcaccgtccg
    21481 ggttacccat gctgaaatcg ataatatctt cgccgcgccg acgcgcagcc attttcagtt
    21541 cagcggtgat gttaaaaacg tagggcggga gacgatcaat gcgcgtaaag cgacgttcag
    21601 ggcgagtgtc agccataatt tcctcagatt aacgttagcg cccggaccgt ccgagcgacg
    21661 ctgccacaat ggtggctgtt ttgaaaatag cctgattaat ttctgtctgt ccagcgtttt
    21721 agaacatttt tatcaatgga tttaaatgag gaactccact tcaaaaacag gaaatttttc
    21781 tacgctaaca agaaaatcac caccaatttt attaacatta taatttcaat aaatttacca
    21841 gccagaagtg cattatcaag agatacattg ctaatacacc atttatcttt taatcccctt
    21901 ttaaatttat ggtttttcct caaaaacggc ctgctggtca tcagccttca ggttacctat
    21961 catagaggtt taatccttat tcagagtcac ccgtgcacga aatattcaac atgctgctgg
    22021 cggtcttcga tcgggcagcg ttaatgctta tctgcctgtt ctttctcatc cgtatccgcc
    22081 tgtttcgcga actgttgcac aagtcggcgc actccccaaa agaattgctc gccgttaccg
    22141 ccattttctc gctgttcgcc ctgttcagca cctggtccgg cgttcccgta gaaggctcgc
    22201 tggtgaacgt acgtattatc gcggtgatgt ccggcgggat tctgtttggc ccgtgggtag
    22261 gcatcattac cggcgtgatt gcgggtattc accggtattt aattgatatc ggcggcgtga
    22321 cggcgatccc ctgctttatc accagcattc tggcgggttg tatatcaggc tggataaacc
    22381 tgaaaatccc caaagcacag cgctggcgct ggatatcgcc aacaaaacgc taccgctgtt
    22441 tcgccatgtc aatagcgagt cattacgcaa ggtctgcgaa attatccgcg atgacattca
    22501 cgccgatgcg gtggcgatta ctaataccga tcatgtgctg gcctatgttg gcgtgggtga
    22561 acataactat cagaatggcg atgacttcat tagcccgact acccgtcagg cgatgaatta
    22621 cggaaaaatc atcattaaaa acaatgatga agcccaccgc acaccagaga ttcattccat
    22681 gctggtgatc ccattgtggg agaaaggggt cgtgaccgga acgctgaaaa tttactactg
    22741 ccacgcgcat cagatcacct cgtcattaca ggaaatggcg gtcggtctgt cgcaaatcat
    22801 ctccacgcaa ctggaggttt cacgcgccga gcagctacgt gaaatggcaa ataaggcaga
    22861 gattcgcgcc ctgcaaagca aaattaatcc ccattttctg tttaacgctc tgaacgctat
    22921 ttcatcgtca atccgtctga atccggatac cgctcgccag ttgatcttta atctgtcgcg
    22981 ttatctgcgc tataacattg aattaaaaga cgatgagcaa atcgatatca aaaaagagct
    23041 gtatcaaatt aaagactata ttgccattga gcaggcccgc tttggcgaca agctgacggt
    23101 tatctatgat attgatgaag aggtgaattg ctgcattccc agcctgctga tccagccgtt
    23161 ggtggagaac gccattgtcc acggtattca gccttgcaaa ggtaaaggcg ttgtcaccat
    23221 cagcgttgca gagtgcggaa atcgggtacg cattgcggtg cgagataccg ggcacggcat
    23281 cgttccaaag gtgattgagc gggtcgaagc gaatgaaatg ccgggcaata aaattggcct
    23341 gctgaatgtc catcatcgcg tgaagttatt gtatggcgag gggctgcata tccgccgcct
    23401 ggagccgggg acggaaattg cgttttacat tcctaaccaa cgcaccccag tcgcctcaca
    23461 ggctacgtta ttgctttgag ccggagtgat attgtgaaag tcatcattgt tgaagacgaa
    23521 ttcctggcac aacaggaact gagctggcta attaaagagc acagccagat ggagattgtc
    23581 ggcacctttg acgacggtct ggacgtgttg aagtttttgc agcataaccg cgtcgacgcc
    23641 atttttctgg atatcaatat tccgtcgctg gatggcgtgt tgctggcgca aaacatcagc
    23701 cagttcgccc ataaaccgtt tattgtgttc atcaccgcgt ggaaagaaca tgcggtagaa
    23761 gcgtttgaac tggaggcgtt tgactacatt ctcaaaccgt atcaggagtc acgtattacc
    23821 gggatgctgc aaaaactgga agcggcctgg caacaacagc agaccagcag tacgcctgcc
    23881 gcgacggtaa cgcgtgagaa tgacaccatt aatctggtga aagatgagcg aataatcgtc
    23941 acgccaatta acgatatcta ttacgccgaa gcgcacgaga aaatgacctt tgtctatacg
    24001 cggcgtgaat cctacgtaat gccgatgaac attaccgaat tttgcagcaa actgccgccg
    24061 tcgcattttt tccgctgcca tcgctcattt tgtgtcaatc tgaacaaaat acgcgaaatc
    24121 gaaccgtggt ttaataacac ctacattctg cgactgaaag atctggattt tgaagtgccg
    24181 gtcagccgca gcaaagtgaa agaatttcgc cagttaatgc atctttaatc gggaactttc
    24241 atgaaagcac caggcttgcc cgccgatcaa caatttttcg ccgatctgtt cagcggccta
    24301 gtgcttaacc cgcaactact ggggcgcgtc tggtttgcca gccagcctgc ctcgttaccg
    24361 gtgggcagtt tatgtattga ttttccccgt ctggatatcg tgctgcgcgg cgaatacggc
    24421 aacctgctgg aagcaaagca gcagcgaatg gtggaaggag aaatgctgtt tattccggca
    24481 cgcgcggcta atttaccgat caacaacaaa ccggtgatgc tgttaagcct ggtgttcgca
    24541 ccgacctggc ttgggttatc gttttacgat agccgcacca cgtcgttgtt gcatcctgct
    24601 cgccagaccc agcttcccag cctgcaacgc ggtgaaggtg aagcgatgct taccgccctc
    24661 acccatctta gccgttcgcc gctggagcag aatatcattc agccactggt gttaagtttg
    24721 ctgcatcttt gccgcaacgt ggtgaatatg ccgccgggta attcacaacc gcgcggcgat
    24781 tttctctatc acagcatttg taactgggtt taggataatt atgcccagcc gctcacccgc
    24841 gagagcgtgg cgcagttttt taatatcacg cccaatcatc tgtcaaaact gtttgctcag
    24901 catggaacga tgcgttttat cgagtatgtg cgttgggtgc gaatggcgaa ggcgaggatg
    24961 attttgcaga aatatcatct gtcaattcat gaagtggcac agcgttgcgg ttttccggat
    25021 agcgactatt tttgtcgcgt tttccggcgt cagtttagtc tgacgccggg agagtacagc
    25081 gcccgttttc agggctaacg tcagaaggtt aattctgttt ccagcagcgt caggatactt
    25141 tgtgcatctt gcgcggcaaa caacgactgg cggaagtttt tattcaccag tttacgcgcc
    25201 agctgcgaga agactttcac atgattcatc ccttcgttag cacccagcgt cagcatgatc
    25261 accagttcga cttcgcccat ttctgactgc cagtcaaccg gtttcgccag ccgggcaatg
    25321 ctgatactgg aatgacggat ccactgagat ttggtgtgcg ggatcgctac gccaaaacca
    25381 acgccggtgg taacaatctc ttcccgctgc cagacatctt cttccagctc aaacggatgt
    25441 tcagtgcgcc cgttaacgcc gaggttgccg cacaggaact ggatcgcctg ctctttattg
    25501 ctaaaatcct gatcaacaaa gatattctcc agcgccagca gtgggcgaac gtcttcttcc
    25561 ggcgtaaagg cggtgagtaa cgcttcaatt tcctgcgcac tgcggcattc acatgcctga
    25621 cgcgccagtt cccgacacgc ctcgctatcc agttgacgaa gctggctttt caccgccgga
    25681 atacgcgggc tactcatact cagctcatcc aggcccagcc caagcagtag cggcagataa
    25741 cggctttcac cgcccagttc accgcaaatg cctacccatt tgccccgctg atgcgcagtg
    25801 gtaactattt gctgcaacat gcgcaggaac gatggcgtaa tcgggttata tagcggcgat
    25861 acgcgcgggt tattacgatc gaccgcatac agatactggg tcatatcgtt ggagccgata
    25921 ctgaagaaat cgacctcatc gcagaagtgg tcgatgatgt agcacaccga cggcacttcc
    25981 accatgatcc caagcgtaat cgtctctgca tgacgcaggc catcgcgctt aagctcaacg
    26041 atcgcttttt ggatctcgcc tttcacccat aagatctgat cgaggctgtg aaccatcggg
    26101 atcatcaact gggcgttgcc gaaactggcg gcgcgcaaaa tggcccgcag ttgagtgcgg
    26161 aacaggccag caaattccgg gtaaatacgt accgcgcgat agccgaggaa cgggttctct
    26221 tcctggggaa tattcagata aggaatgctt ttatcgccgc cgatgtccat cgtgcggaag
    26281 ataatcggct tgtcgcccgc cgccagtagc acctgctggt aggcttcaaa ttgctcctgc
    26341 tcgtccggcg cgctgtcgcg atccatatag agcatttcgg tacggaacag cccgacacct
    26401 tccgcgccgt tggcaaacgc gcctggcgct tccagagcgg tgccgatatt cgccgcaata
    26461 tcaatacgct tgttatcacg ggaataggcc agctgcgcgg cagcctgcgc ctgttgtttt
    26521 tggcgtttat ccgccagcgt ctgcgcgacc tgataataac cgctcaccgc gtcattcggg
    26581 ttaatcgcca gcacgccgca ctgggcgtca agcactgcag gttgcccggc ataacgggca
    26641 atcgcatcca gcggcaagcc actcagtacc gggatcgccg aggcacgggc cagaatcagt
    26701 gtatgcgagg tgcgcccggt tttctccaga atcatacccg caagattttt caaatcaagg
    26761 ctcaaaaact ggcttggggt taaatcttca gccaccagaa tggtcggttt ttccagcacc
    26821 agattgttgc gcggcttcag ttccggccag gtgatatgca gcaactgttc gctgatatcg
    26881 cgaatgtcgc tgacccgctc gcgcagataa tcgctggcag aagcagatag tttggcgcaa
    26941 acctgctcca tattgctgat gatcgccgcc cccagtccct gatgctgttc tgtcatcagg
    27001 cgacggatat tgcctgcaaa ttcatcatcc tgaatcagcg acaaatgagc gctgaggata
    27061 gttttgcttt cgccgtcacg ctcacgcaat tgctgattca gttgctcggc aagcgttgcc
    27121 aggctgtgct ccagccgggt ggaatcttgc gcactggcgg ggattgcccg ataactgtcg
    27181 aggctgtcgc tttgtaacag ggtcagcgta cccacgccga cgccgcttgc cagcacattg
    27241 ccgtacagta aatccgggtt aaggcggctt aatgaacgcg gcagcggatg cgccgtcagt
    27301 tctgcctgcg tcggctgaac gctgtcgctg tcgataaagc gcaccaggat gtactcttcc
    27361 agcacgcgcc gcgcctgctc ttcatcgctg ccgctaatgt tcaggctgca actgtcatta
    27421 aataaggtgc cagttccaat caacgccagc gagcttttag catcagcctt tgcgttctgg
    27481 cgatggttga taaaggtgat ttcgctttgc cactggctgc actgttcttt aagttcccac
    27541 gccggacgag catgtagacc gttaggcaaa ggacagagaa attgaatcgt taacataacc
    27601 gctccgggtt aatcaggaga tcatctgaaa tccgtcagtt gaacaaccgt ctcacgcgta
    27661 agacgttgaa taagggcaga taaaagttgc tccatctgca aaatgtcgcg gcaatcggca
    27721 atcgatgccg cgcaatgtcc gtggcgggtg gcaggcccca ttaccaatgt gggtacgcca
    27781 gtaccggtta agtgcaccgc tccaccgtcc gtgccgccgt tactgaacat atccgcctgc
    27841 aacggaacgc caatttctgc cgccacggtt tcaatccacg cggtaagttt tggcggcgca
    27901 atcagcgatt tatcgcttaa caccagcatc ggcccgttac caatctgacg atggttagcc
    27961 gcgccataat caaagttttt cgcccagcag gcggtatcaa gcacaatggc gacgtccggc
    28021 gacaccgcgc gggtagcagt ttgcccgccg cgtaatccca cctcttcgct ggaacttgcc
    28081 accagccaca cttccgcagg cagttcggcg tcgtgcagct cacgtagtaa cgtcaccagc
    28141 agatagcaac tgaggcgatc atcaaaggct ttgcccatca cccgctggtg agggaggacc
    28201 tgaaaagtgg tatcgaaggt gacgcgatcg cctggacgaa ttcccgcctg catcacttcg
    28261 tcataggtgc gcgcgccaat gtccacgcgc atggcgctaa cgtcattccc ctgccggtcg
    28321 ccgtcaagca ggcctggaat tttgcactct tcacgggtgg tgatgcgcac cggctgcagc
    28381 tggcgggcag ccatgcgcac gttgccaacc ggcagcacgt caatagcgcc ttcgcgggag
    28441 atgctgcgca ccataaatcc cacttcgtcc atatgggcgc agatcatcac cttcggacct
    28501 gtcgattcat tgaggcggat cagcaccgat cccagaccat caaatcgcac ttctttttgc
    28561 aggcgagccg cttcttccag caggatctgc cgcacttcct gttccgagga ggcgatcgca
    28621 tctgcctcgc tcaacgcttt taatagcgat aaatccatta tgcctctccc gtgagcaaca
    28681 ctgttttcgg catggcgtag agcacttctg cgccttgcgg ggtaaccagc acaacatctt
    28741 cgatgcgcac gcccccttgc cctagcaaat aaatccccgg ctccacggtc agcaacatgc
    28801 ctggctgtag cgtcgtggtg tcccgcggtg aaaaacgcgg atcttcatgg acttcaatgc
    28861 cgatagcgtg accagtgtta tgaccgaaat agtcgccaaa tccagcctcg gtaatgaccc
    28921 ggcgcgcggc gtcgtcaatc tgctggcagc gcacgccggg gcgaattgca gagattgccg
    28981 cgagctgtgc ctgcaggaca atctgataga cgtcaaacag cgggtgagat tcggcgctca
    29041 ccccttcgcc attcaccagc aaggtgcgcg tcatatcagc agtagccctg atacagcgca
    29101 ccaaaatcga gagtgacaaa ctcgcccgct gcaacaatct tgtcgctggc tttgccgtgc
    29161 ggcagcgccc cgcgccagcc gctggcgaca atggtgtcaa aagaggcttt ttctgcgccc
    29221 tgctggcgca taaaccactc cagttcagcg gctatctcgc gctcgctcat ccccgcctga
    29281 ataaagcggc gaatatgctc cgctccgcga tcggcaatcc cacaggcgag gcggattatc
    29341 tccacctcct ctggcgtttt gatttgccgc agcacatccg gcgtggcgct aaccagtttc
    29401 gcattgagtt cagacttcca gcggtgcgcg gtttcccaac tcacctgctg gccttcaaaa
    29461 ccgagcgttt gcaactgctc atcggcaatg atttgattga cgatagtggt aagcgtgttc
    29521 gttgcgtcaa gcaaatgcag ctggtagcct tgcgcacggg cttccacctc aacgaaatag
    29581 cgcgaatcta ccagaatgtg cgcactttca cggctaataa ccacatagcc tgatccggtg
    29641 gagatcccca gatgcggctg tttgttctgc cgtgaggaga gaagcaccgc atccagttgt
    29701 tgcaccttaa gccagttgcg cagcgaagcg agtaatgtca tctggatttt ccttcttaca
    29761 ggctatcgat taacaatttg cctttgcgga acatcatcag acgcaggaac accaccatca
    29821 gcgcagtaat gattgccccc agcgcgatcc ccgccatata aacgcccaag ttagtgacca
    29881 gcggccatgc ccagatagcg gattccggga accattgcac tgcgcccagc cagacagctg
    29941 cggttgagcc gacaatcgcg ccgaccatat atgacggaat agcggtgatg gggctttcca
    30001 gcgcaaacgg aatcgcaccc tcactaatcc ccataaaggc caggaacatc gcggtcttac
    30061 cctgcggata aagttgagcg ttgaacaggc gtttgccggt taaacgacgg tcaataatgg
    30121 tcgccagacc cagaccaatc ggcggaataa cgatagcgat agaacgagcg gtgaccggta
    30181 atacgtggtc agtggtaaag ctgaaggcaa cgaaacctgc ggctttgttg atcgggccac
    30241 caaggtcgat tgctgtcgcc gcggcgatcc ccattgcgta catcagcgcg cccttctcac
    30301 ctgcggcagt cagcacagta cggataccgc cgttgatcca gccaccaaag ggagtgatga
    30361 cgtagtacat cgccagcatg acaaaaatgg cggaaagaat cggtagcaaa aatgtggttt
    30421 tgaacgctaa caggaaatcc ggtaactgga ttttctggtt catccatttc acaaggtaac
    30481 cggcaacgat agaaataatc agcgcaccaa tgaaggtgga cggcaccggc gaagaggtcg
    30541 cccattgcat agtgctggga tcgaagttca gcagttgcgt cggctgggta gacatcaacc
    30601 cgccgataaa accagccggg aacgccagtt tgccaccgat agagttcgcc acaaatgcag
    30661 caaacatcgg aatggcaaaa ccaaacaaca cgccgccgaa ggactgcgac agccaggcaa
    30721 atttaagtaa ggagaggtcg aaaccggaga atttcccgct attaagagca tccatgatgc
    30781 cgatctcagc aggaatttta agccagctat aagcaatcag ctggctgaaa gcgaggatca
    30841 caccgcccat aattaaggtc ggcaccatgc gtgaaatccc tgacatcaca tgctgcggga
    30901 gttcacccca gaagctgctt ttagaggcct gcggattctt aactgccgct gccgcaccgg
    30961 atgcgccagg cacaacggtt gcactgcgtt ttttaatggc cataacaatt atccctgtcg
    31021 aatattactg ttgttcagag gcaatcatct cttcgatttc tttgatgatg cccgcagcgt
    31081 ttttaattgc gtcctgcaaa gtgatttcat aaacgtcgcg tgattcgaaa cgttcgttat
    31141 cttccggggt aactgccacg gaatggatga tgatggtcgc ttcggcgata tcctgcgccg
    31201 tcaggcgatt ctggatgccg tccgcgccct gggtttcaat tttcacttca taaccggctt
    31261 ctaccgccgc ttcttccagc acctgagcgg ccataaaagt gtgagccagg cccattgggc
    31321 aggcacataa ggcaatcagt ttcttactca tcgttaattc ctcgttcagt gatagttgac
    31381 tagtgaaatt atgaacgtgc aaaacaaatc gccgttacca gacaaataat gcatttactg
    31441 gagaattaat cccgctgtac ggaagtgtga cgcaggtcgg ccttgtgagg ggacacaaag
    31501 aaaaattgaa ttaactgtat gatttaaaag attatcggga gagttacctc ccgatataac
    31561 aggaaggatt tacagaatgt gacctaaggt ctggcgtaaa tgtgcaccgg aaccgagaag
    31621 acccggatag tcatggacga tgagatacac cggaatatca tggacatatt ctttaaagcg
    31681 ccctttatct tcaaatgcgg cacggaaacc ggaggctttg aagaactcaa ggaagcgcgg
    31741 cacgataccg cccgcaataa acacgccgcc aaatgtcccg agattgagcg ccagattgcc
    31801 gccaaaacgg cccataatga cgcaaaacag cgacaatgcg cggcggcaat cggtgcagct
    31861 gtcagccagc gcgcgttcgg taatatcttt tggcttgaga ttttctggca ggcggttgtc
    31921 agctttcaca attgcgcgat acaaattcac cagcccaggg ccagaaagca cgcgctccgc
    31981 cgaaacatga ccaatttccg cacgcaatat ttcgaggata atggcctctt cttcactatt
    32041 cggcgcaaaa tcaacgtgac cgccttcgcc cggcaagctt acccaacgct tatcgacatg
    32101 gaccagatgc gcaaccccaa gccccgttcc ggcaccgtaa accgcaatag gcttaccttc
    32161 gaccggttct gcgccaccaa actgaatcag atgctctttt ttcagcatcg ggatcgccat
    32221 cgatacagcg gtaaaatcgt taataatttc cagatggcta aaaccgagat tctttttcat
    32281 ttcggcaatt gagaacgccc aggtatggtt ggtcattgcc acccagtcac cggtaattgg
    32341 gcaagcgatg gcaatacagc cgtctttcac ctcgacctta tgttcttcaa gataaacgcg
    32401 aatgaccgct tcgaggctgg ggtaatcaag ccctgaatag gtcttagcct gcgagatttc
    32461 accactggca atatcacaca gagcaagacg tgcgttggtg ccgcccacat caccgactaa
    32521 tgcatacttt gtcattcttc aactgctccg ctaaagtcaa aataattctt tctcacactg
    32581 taaatacctg ggggcataac aacaacgccg gaaaggcagg ctccctgtaa atatcgatct
    32641 gggtcacaca attactttat cgtttcagca ccaattgcag cgatgccttt ttgcaagctg
    32701 ggcaaactaa gtatctgacc ccgcataagg aatagaacat gctccatccg cgagccagaa
    32761 ccatgttgtt attatcgctc cccgccgtgg caattgggat tgcgtccagt cttattctga
    32821 ttgtggtgat gaaaatcgcc tcggtattac agaatttgct ctggcaacga ctgccgggaa
    32881 ctctggggat agcccaggat tcacccctct ggatcatcgg tgtattaacg ctaacgggta
    32941 ttgcggtggg gttggttatc cgtttcagcc agggtcatgc cggaccagac cccgcctgtg
    33001 aaccgctgat cggcgcaccg gttccgccct ctgcgctacc tagacttatc gtagcattaa
    33061 ttctcggtct tgctggcggc gtcagcctgg ggccggaaca tccgatcatg accgtcaata
    33121 tcgcccttgc cgtcgccatt ggcgctcgtc tgttaccgcg cgtcaaccga atggagtgga
    33181 ctattttagc ctctgccgga accatcggcg cgctgtttgg tacacctgtt gcggcggcgt
    33241 tgatattttc gcaaacctta aatggcagta gtgaagttcc gctatgggat cgtctttttg
    33301 cgccgttaat ggcggcagca gctggtgcac ttactaccgg attatttttc catccccatt
    33361 tttcactgcc cattgcgcat tacggacaga tggaaatgac cgatattctc agcggtgcaa
    33421 ttgtcgcggc gattgccatc gcagcaggga tggtcgccgt atggtgctta ccacggttgc
    33481 acgcgatgat gaatcaaatg aaaaatccgg tgctcgtgct gggtattggc ggatttattc
    33541 tcggaattct gggggttatt ggtggaccag tttcgctgtt taaagggctg gatgagatgc
    33601 agcagatggt ggcaaatcag gctttcagca ccagcgatta ctttttgctg gcggtaatta
    33661 aacttgccgc cctggtcgtt gctgccgcca gtggctttcg cggtgggcga atcttcccgg
    33721 cagtgtttgt cggcgtggca ttagggttga tgctgcatga gcacgttccc gccgttccag
    33781 cggcaataac cgtttcctgc gctattctcg gcatcgtgct ggtggtaaca cgcgatggct
    33841 ggttaagtct ttttatggcg gcagtcgttg tacccaatac cacattgcta ccgctgctct
    33901 gtatcgtcat gcttccggca tggctgttat tagcaggtaa gccgatgatg atggtcaatc
    33961 gtccgaagca acagccaccc cacgataatg tttagcaaaa aatgctctcc tttatgattt
    34021 aagagttatg gcgatgatac ggggctttac ctccccgtaa tattgcgtta acaggccgct
    34081 gacggcttcg atcgtgaagg agaataacaa tgttcaggtc actgtttctg gcggccgccc
    34141 tgatggcatt taccccgctt gcagcaaatg caggtgaaat caccctactg ccatcgatca
    34201 aattacaaat tggcgatcgc gatcattacg gtaattactg ggacggtggt cactggcgag
    34261 atcgtgatta ctggcatcgc aattatgaat ggcgcaaaaa ccgctggtgg cgtcatgata
    34321 atggctatca tcgtggctgg gataagcgta aagcatatga gcgtggctat cgtgaaggct
    34381 ggcgcgatcg tgacgatcat cgcggaaaag gccgcggaca tgggcaccgc cattaaacgc
    34441 gtcctccaat ggagcacaat gcctgatgcg acgctagcgc gtcttatcag gcctacaaac
    34501 cggctcttta acgcgtaggg cggataaggc gtttacgccg catccgccaa tagtgccaga
    34561 tgcgacgctc attcaactac aatcccagcg ccgtccccac caacaaccag atattcagcg
    34621 ccacgaccag cactacaatc acccagcctg tctgttttac gcgtttgctg ttcaccagat
    34681 cgcccatcaa cttgctgtca ctggtgaaaa tcagcagtgg aaccagcgcc agggcgatac
    34741 caaaacttaa cagcacctga ctcataacca gaatccgtgt cggatctaat cccatcagaa
    34801 tgacaataaa tgacggcaac atggtgactg tacgacgcac ccacagcggg atatggaagc
    34861 gaatgaatcc ctgcatcacc acctgccccg ccagtgtccc caccaccgtt gaggacagtc
    34921 cggcagcaac cagacttaac ccaaagaccg ttgccgcagc atggcttaac agcggttgca
    34981 gcgtcagata agcctcatca agatcggcaa caccagtatg accagaaaag tggaacgccg
    35041 ccgcagctgt agccatcatc accagattga caaaaccggc aatcgtcatg gcgatagcca
    35101 catcccattt ggtggcggaa taacgttgtt gacgcgaacc gccatgtaaa tgctgagtga
    35161 gcgaagagtg caaataaatc acatgcggca taatcgtcgc ccctaacacg cctgctgcca
    35221 ggaagaccgc ctccgaagta ggtaaactcg ggatcaccat tcctttaccc agctgcgcca
    35281 ggttaggctg ggagaaaatc aactcgacaa tgtaagccgc ggcaacaaac aacagtaacc
    35341 cgccaatcac tttctccagc ggtttttgcc cgcgacgttg cagcattaaa atcaggaaag
    35401 tcgcgatccc cgtcagcacc gcgccctgca acaacgaaac accaagaatg agtttaaaac
    35461 cgatcgccgc accaataaat tccgccaggt cggttgccat cgcaataatt tctgcctgaa
    35521 cccaatagaa ccacactacg ggacgcggat agtgatcacg aatctgctcc gccagatttt
    35581 taccggtggc aatccctagt ttggcagaga ggatctgaat cagcatcgcc atcaggttgg
    35641 cccaaacgac aacccacagt agctgatagc cgaagctagc acccgcctga atattggtcg
    35701 caaagttacc gggatcgata taaccaatcg ccgcaatgaa cgcaggtccc attaatgcga
    35761 gcctcatctt acgcgccgcc cgtccgctgc tactctcaac gcgatagttc gtcatcttgt
    35821 gcctctaaaa catagccttt gctatgtttc atgctatgct aaacgagaat gattatcaaa
    35881 ttcatttaaa tggattgtgg tgatttctct gatagaccaa gattatgatt acgaaaagat
    35941 tgatgacttg aatgttatgg ttatgtttaa tgttagcaca tttacataac tttcagcttc
    36001 catacacaac atagcagaaa tgtatgacag atcactattt ttgaagcctg tcacaggacg
    36061 tcattatagt gtgtgtcaga tctcgttttc cttaaccatg ttacatagaa tgtgcacgga
    36121 aatttaacct gcctcatatt tggagcaaat atggaccgcg tccttcattt tgtactggca
    36181 cttgccgttg ttgtgattct cgcactgctg gtaagcagcg accgcaaaaa aattcgtatc
    36241 cgttatgtta ttcaactgct tgttatcgaa gtgttactgg cgtggttctt cctgaactcc
    36301 gacgttggtt taggcttcgt gaaaggcttc tccgaaatgt tcgaaaaact gctcggattt
    36361 gccaacgaag ggactaactt cgtctttggt agcatgaatg atcaaggcct ggcattcttc
    36421 ttcctgaaag tgctgtgccc aatcgtcttt atctctgcac tgatcggtat tctccagcac
    36481 attcgcgtgt tgccggtgat tatccgcgca attggtttcc tgctctccaa agtcaacggt
    36541 atgggcaaac tggaatcctt taacgccgtc agctccctga ttctgggtca gtcagaaaac
    36601 tttattgcct ataaagatat cctcggcaaa atctcccgta atcgtatgta caccatggct
    36661 gccacggcaa tgtccaccgt gtcgatgtcc atcgttggtg catacatgac catgctggaa
    36721 ccgaaatacg tcgttgctgc gctggtactg aacatgttca gcacctttat cgtgctgtcg
    36781 ctgatcaatc cttaccgtgt tgatgccagt gaagaaaaca tccagatgtc caacctgcac
    36841 gaaggtcaga gcttcttcga aatgctgggt gaatacattc tggcaggttt caaagttgcc
    36901 attatcgttg ccgcgatgct gatcggcttt atcgccctga tcgccgcgct gaacgcactg
    36961 tttgccaccg ttactggctg gtttggctac agcatctcct tccagggcat cctgggctac
    37021 atcttctatc cgattgcatg ggtgatgggt gttccttcca gtgaagcact gcaagtgggc
    37081 agtatcatgg cgaccaaact ggtttccaac gagttcgttg cgatgatgga tctgcagaaa
    37141 attgcttcca cgctctctcc gcgtgctgaa ggcatcatct ctgtgttcct agtttccttc
    37201 gctaacttct cttcaatcgg gattatcgca ggtgcagtta aaggcctgaa tgaagagcaa
    37261 ggtaacgtgg tttctcgctt cggtctgaaa ctggtttacg gctctaccct ggtgagtgtg
    37321 ctgtctgcgt caatcgcagc actggtgctg taaaaccata cataaaaaag ccggggatca
    37381 ttccccggct tttttacgcc tgttaatcag ctaatggctg cgggcgacca atcaaatacc
    37441 cttgcagata ttgcaccccg agcttatgca atagcgcctg ctgctgttgc gtctcgacaa
    37501 actccgcgac cacactcaat gacttcgctt tcgccagatc ggtaattgat ctcacaatca
    37561 tcgcatccag cgtattcgtg acaatatctt tcacaaagac gccatcaatt ttgatgatat
    37621 cagcctgcaa acgctttaac cgttcgtagt tggcatatcc ggtgccaaag tcatcaatcg
    37681 caatccggaa accaaactta tgcagctgct cgatgttgta catgctgctt tctgcgttag
    37741 aaaacgcctg ctcctcggtg atctcaagaa tgaccgcctg cggggagatg tgataacgct
    37801 taaacagacg aataatccgc ccggcaatat tcttttgcag cagcgtgagc ggcattaaat
    37861 tgactgaaaa gcgcggtcct tttttgtcgc aagggtgtgt tgccagccac ttcaacaagg
    37921 attccagcac ttgcaaatca aaacgcgcgc taaggttaaa ctgagcaata aggggcagaa
    37981 acttatccgg ggtcataatg ccgccgtcat atttcagtcg cgcgaggatc tcatcataac
    38041 cttcaccctc tttgttgcga attggctggg cgtagagcag caaatcaccc tgatctaacg
    38101 ccgtgcgaat ggtattcagc aatagcacct gtttagtggt ctgcccggaa accatctcct
    38161 ctctgctatc cagcgccagc acatgatgat gtgcgcagga ttgctccgcc agccagctta
    38221 actgccccaa caagggttgc agggtttcct gatttccatc aaaacgcccc caggcagcac
    38281 catagcccat atccagcccg gtattgttcc agtgaatttg ccgactattc aggatgttaa
    38341 ccatatgctg gagtcgccct tccgtttccg gcccgctcag cactaacagc agttcactac
    38401 ccggcaattg atacaacttt tcgttttcct gcatcaacgg cagcagcgta cggcaaattg
    38461 agcggataca gtgaacgcgc atcattaagc cgtaatgacg actcataaac tcaagattat
    38521 caatgcgcag gcagcaaaaa ctcttgcccg cctcttgctc cggcgcttgt tccaacgcac
    38581 gaaagttggg taggagcgtt aacggatctg ttagcgcctg caaatgccag cggcgattaa
    38641 gccattcact gcgatgataa atgcgcacca tatagagcag gcaaacgcta aaggaaatca
    38701 gcaccgcgag aataaatgcc agcgaatatt cggtttccac cccttgcaaa aagttctgat
    38761 tgtaattcag aaggcaaagc gttgaaacag cccaggtgag atttaaaaac ggatagcgaa
    38821 gattaccgac cccgagggta aagatgatga agaaaacggg taccaggtaa ccggcaataa
    38881 agtcgttttc ataaggtgtg cacaacagaa gtagcagcac gcttaacgct gccagccagc
    38941 ttaaggtaaa tgcgagtgag atagtagaaa agcatgttgt aaatcagcac tgcggtgaaa
    39001 aggcttagta aatcaacgac cgtgaaaatg gcatccgcat cgccgaaaaa ggtggatatc
    39061 tttagcggga aatcaaagaa gctccccaca agatacatgc tgcatttgat gccgatcggc
    39121 gtcaccaaac caagccagaa cagacgttgc cagacatatc ggctggtcaa tccataacgc
    39181 caacgcgtcc ccagctgcca gcgtaatatt gcgcaggcac acaacacgac aaacgtctgg
    39241 ctgccgagca tgaccgcagt ctgtaaaaaa gagagatgaa agttccacag attggtgcaa
    39301 aacatcccgc ataaaacggg aacgacaccg cgccagccaa agataaacaa gattgacagc
    39361 attacgcaca gtggcatcca cgccagaaag atataactgg aatggataat ggcaagtggc
    39421 gaaataaaac gggatagctg aataagtacc acggtgagcg taaacgctag tgtgaatatc
    39481 ttgatatttt ttatcaggtt atgctccaca aacatgagta cactactctc actgatcggg
    39541 gttttcaggc acacaaataa taagtttatt cgcatcgcga agcaaacagg ggccagcctg
    39601 ttgccgattt ttcatccaat gatgctttca ccaggcggcg ctaacccgga cgtagactgt
    39661 attacaaaag cggcaaaaag cagagacaaa aaacccccgc tttgcagcga gggttggaaa
    39721 tttggtggag ctaagcggga tcgaaccgct gacctcttgc atgccatgca agcgctctcc
    39781 cagctgagct atagccccac gatgcgttta cgtaccaaat ttgctgggtg caaaatttgg
    39841 tggagctaag cgggatcgaa ccgctgacct cttgcatgcc atgcaagcgc tctcccagct
    39901 gagctatagc cccgtcacgt aaagcttgtc gagttgacgg gcggcatcat atgaattccg
    39961 cccgaatgtg tcaacggcaa attgcaacgt gtagtttcaa tcgctgaaaa atcaggcaaa
    40021 tgaacaattt tggaaaaccg ctcgcattca gtagttattc atgtcacggt ttcctgtaaa
    40081 gtggtgttat aaaatgaact actaatagac ccacatacat tcagggaatt gttatgttca
    40141 aggagaggat gacaccagat gaacttgcca gactgaccgg ctatagccgc cagaccatta
    40201 ataaatgggt acgcaaggaa ggctggacaa cgtcaccaaa acctggcgtc cagggtggca
    40261 aagcccgact ggttcacgtc aatgaacagg ttcgtgaata tattcgcaat gctgaacgtc
    40321 cagaaggtca gggagaagcg cctgcccttt ctggtgatgc gccgcttgaa gtattgctgg
    40381 tgacactggc gaaagagatg acgccagttg aacaaaaaca gtttacatcc ttgcttctgc
    40441 gggaagggat tatcggattg ttacaacgct tagggattcg cgatagcaaa taatatgaaa
    40501 agattacgca ataaaatgac caccgaagaa ctggctgaat gcctcggtgt ggctaaacaa
    40561 actgttaacc gttggaccag agaaaaaggc tggaaaacgg aaaaatttcc cggcgtgaaa
    40621 ggcggtcgtg ccagacttat tctggtcgat acgcaagttt gcgagtttat tcagaacacg
    40681 ccagccttcc ataacacacc aatgttgatg gaagctgaag agagaattgc tgaatacgct
    40741 cctggagcac gcgccccggc gtatcgacaa atcatcaacg cgatagataa tatgacagac
    40801 attgagcagg aaaaagtcgc gcaatttttg tcacgtgaag gaattcgcaa tttcctcgcc
    40861 cgtctcgata ttgacgaatc agcataaata aaaaacggca ggatattatc tcctgccgtt
    40921 tatcttttta cacgctaatt actgctgatt ttcgcgttca gcaataaaat ccagcgcttt
    40981 gttgatacgc tcgatactgc gggtcttacc aattgcgtga acggtaacat ccagtgctgg
    41041 agactgcccc gcaccggtta cggcgacacg cagcggcata ccaactttac ccatacccac
    41101 ttccagctca tccgccgtcg cctgaatagc gtgatgaacg ttttcagcgg tccagtcagt
    41161 aatcgcggcc agtttgtcac gaaccacttc cagcggctga cgcgctaccg gacgcagatg
    41221 ttttttcgcc gcatcggcat cgaactcagc aaaatcttcg tagaaataac ggcagctctg
    41281 tgccatctct ttcagcgtct tgcagcgttc gcccagcagt ttcaccagat cagccagctg
    41341 cgggccgtta cgggtatcga tattttcctg ctcaatgtgc cactgtaagt gagtagcaac
    41401 atactccggc ggcagcgcgt taatgtagtg atggttcagc cacagcagct tgtcggtgtt
    41461 gaacgcactg gcagatttgc tgacggcatt caaagtgaag tatttgatca tctcttcacg
    41521 agtgaagatt tcctgatcgc cgtgggacca gcccagacgc accagatagt tcagcagtgc
    41581 ttctggcaaa taaccgtcat cacgatactg cattacgctg actgccccgt gacgtttgga
    41641 cagtttttta ccgtcatcgc cattgatcat agaaacgtgc gcgtaaaccg gcaccggcgc
    41701 tttcagggcc ttaagaatgt tgatctggcg cggcgtgttg ttgatatggt cttcgccacg
    41761 gataacgtgg gtgatttcca tatcccagtc atcgacaacc acacagaagt tataggttgg
    41821 ggaaccatcg gtacggcgga taataagatc gtccagttcc tggttgctga actcgatcgg
    41881 accacggatc tgatcgtcaa aaacaacaga accttcctgc gggttagcaa aacgtacaac
    41941 acacggttca tcatcagcat gatgctcatg gctgtggcgg cagcgaccgt cataacgcgg
    42001 cttctcacct ttcgccattt gctcttcgcg cagcgcttcc aggcgctctt tagagcaata
    42061 gcatttataa gcagtgccct cttccagcat ctgatcgatc accgcgttgt agcgatcaaa
    42121 acgtttggtc tggtagtacg gaccttcatc ccactccagg ctcagccagt tcatgccatc
    42181 cataatggct tcgatagctt ccggcgtgga acgctcaaga tcggtgtctt caatacgcag
    42241 cacgaactca ccgccgtggt tacgtgcaaa aagccaggag taaagagcag tacgcgcgcc
    42301 gccaacgtgc agatagcctg ttgggcttgg cgcgaagcga gttttgattt tcatgaaatg
    42361 gccttacgtt tagaaagatg ccgacaaccg gcaaatcctg gaaaaattga gtgggcgata
    42421 ttctatcact cccgcctgat tcctcaatgt agttcgggtt tctaccttac gcattattgt
    42481 tttcgttgac aaattgcgca acacggtctg ttttgcgatc atcttgctta aatttacgac
    42541 gaacgaacaa tttctttaga aaatgcgttg actcattttg aactctccct ataatgcgac
    42601 tccacacagc gggggtgatt agctcagctg ggagagcacc tcccttacaa ggagggggtc
    42661 ggcggttcga tcccgtcatc acccaccaac tactttatgt agtctccgcc gtgtagcaag
    42721 aaattgagaa gtgggtgatt agctcagctg ggagagcacc tcccttacaa ggagggggtc
    42781 ggcggttcga tcccgtcatc acccaccact tcgggtcgtt agctcagttg gtagagcagt
    42841 tgacttttaa tcaattggtc gcaggttcga atcctgcacg acccaccaat gtaaaaaagc
    42901 gccctaaagg cgcttttttg ctatctgcga tactcaaaga ttcgaacctg cagcaggttt
    42961 gagttgagcg cagcgaaaca acggagccgc tcgcggcgac ggcccgaagg gcgagcgaag
    43021 cgagtcatcc tgcacgaccc accaatgcaa aaaagcgccc taaaggcgct tttttgctat
    43081 ctgcgatttg taagattgat ttttcttctc aagttccttg ttcttctacc gatattacct
    43141 cgtacagtga cgaggactga acaatggcca ctatctcttc aacttcaatt ccttccatac
    43201 aaacacagag tagtaacagg gcatcacaag gtagcgatgt tgcttcacaa atcgcgcgga
    43261 taagtcagca aatcatcaaa ctcactcaac aaattaaaga gattgtagac actagcggca
    43321 gtgctgaaga caagcaaaaa caggccgaat tgattcaaca gcaaatcacc ctactggaaa
    43381 cacaactggc gcagttgcag aaacagcagg cagaaaaggc gcaggagaaa gagcagcgtt
    43441 tgtctctgaa tgtatccctt ctaaatcctg ttgaaaaaac gactcatatc gatatctata
    43501 tctgatacaa cggatcccct tcccgaccag ccattagcgc attgccctgg tctcgtacca
    43561 cgtcccataa cgcttcagcg gcggtggaaa gtgaacggtt cttcctgcgc accagcatca
    43621 actggcgttc aaccaccggc gtaatgcgct taaccaccag tgggctacct tcaggcaatg
    43681 gtagcgccag tgcaggaaga atactgatgc cgatgcctgc ggctaccatc gggaacagcg
    43741 tcgccggatg tccaatctcc tgcacaatat tcgcctgaat accattgcgc gccagcgctg
    43801 cgtcaatcag cggtcggctg cctgacgcgt aatcctgcaa caccaatttc gctccctgta
    43861 atgcctgcca gggtacataa tcttccacgg ccaaagcgct atcgcgatgg caaagcagaa
    43921 agaaaggctc ggaaagaatc gcttcacatt gcagatcgcc cactgggcct ggatcgatga
    43981 cgatgccaaa gtcgacatct ccctggcgaa tactttccat cacccactgc tgcggacgat
    44041 catgcaggac aaactgaata tctggatagc ggcgatggct ttcagcaatg cattgcggaa
    44101 taagatgcgc ggaaatggtc tggctggcag cgacccgaac ttttccgctc agttgttgcc
    44161 ccatacgccc ggtatcgcgc aacgtgctgt taagttcatc cagcagtcgc tcaagacgca
    44221 aagccaactg ctgccctgca tctgtaagca ccacttcacg cgtggttctg tccagcaggc
    44281 gaacaccagt atgattttcc agctccttca cactgtgact tactgccgac tggctcaagc
    44341 cgatacgctc tcctgcacga ctaaaacttt tctcctgcgc tactgtgacg aaaaccttta
    44401 attgttttaa agaataattc atctgttttc ttcatgaatg gatgcaataa atcaatttta
    44461 tttctcaaac tggaagaagc acaatagaac catcgatcat ctggagtctt tatgaaactt
    44521 tttcgtatcc tcgatccttt caccttaacc ctgatcacgg tggtgttgct ggcctctttc
    44581 tttccggcca gaggcgattt cgtccccttc tttgaaaatc tgaccaccgc agctattgcc
    44641 ctgctgttct ttatgcacgg cgcgaagttg tcgcgtgagg cgattattgc tggcggtggt
    44701 cactggcgac tgcatttgtg ggtaatgtgc agcaccttcg tgctgtttcc gattctgggt
    44761 gtactgtttg cctggtggaa accggtaaat gtcgacccga tgctctactc cggttttctc
    44821 tacttgtgca ttctcccggc taccgtgcag tctgcaatcg ccttcacgtc aatggcgggc
    44881 ggtaacgtcg cggcggcggt ttgttctgcg tcagcatcca gcctgctggg gattttcctt
    44941 tcaccattgc tggttggtct ggtgatgaat gttcacggtg caaggggcag ccttgagcag
    45001 gtcggtaaaa ttatgctgca actgctgctg ccgtttgtgt tggggcatct ttcccggccg
    45061 tggattggtg actgggtgtc gcgcaataaa aaatggattg cgaaaactga ccagacgtcc
    45121 attctgttgg tggtttatac agcgttcagc gaagccgtcg ttaatggtat ctggcataaa
    45181 gttggctggg gatcattgct gtttatcgtg gtggtcagct gcgttcttct ggctatcgtg
    45241 attgtagtta acgtctttat ggcacgccga ctgagcttca ataaggcaga tgaaattact
    45301 atcgtctttt gtggttcgaa aaagagtctg gcaaatggca tcccgatggc aaacattctg
    45361 ttccccacat cggtgatcgg tatgatggtg ctgcccctga tgattttcca tcagatccaa
    45421 ttgatggtct gtgcggtgct ggcgcgtcga tacaaacgcc agaccgaaca gttacaggcg
    45481 cagcaggaaa gcagcgccga taaagcttaa agcggacgct tcaggggctg gaccagttgc
    45541 gtcagcccct cggttttaat cagcagcgtg atttgcatta gctcaccgag cttccctgcc
    45601 gggaactcat ctttgcgggc aaaccacagc agatactcct ccggcaggtc aattaagcga
    45661 cgccctttgt atttgccaaa cggcattatc gtattggcta tttcaatcag ctgctctttt
    45721 tccatctcag ctacccagca aacgcagcat ttccgcttcg tcgatgactt caatgcccag
    45781 ttcctgcgcc ttcgccagtt tagatcctgc agcttcaccc gctatcacca gatcggtttt
    45841 cttcgacacg ctgcccgcga ctttcgcgcc cagttcgacc agtcgagctt tagcgtcatc
    45901 acgcgacatc tggcttaagc tgcccgtaag caccacggtt ttaccagcaa acgggctgtc
    45961 aatctcttcc gcgttgataa cgatcggcgc aggccagtga acaccttccg ccaacagctc
    46021 gctgatgaca ttgcggttgc tttcttcggc aaagaagttg tgaacgtggg atgcaacgac
    46081 aatgccaaca tcaggcacct tttgcagctc ttcaatcgaa gcggcttcca gcgcttccag
    46141 cgtgccgaaa tatgccgcca gacctgctgc ggtggcctcg ccgacttcac ggatgccaag
    46201 tgcatagagg aagcgagcaa aggtggtttc tttcgctttt tccagcgcgt taaccacgtt
    46261 ttgtgccaat tttggcccca tacgctccag tccggtcagt ttgcctgcgg tgagtttgaa
    46321 cagatctgcc ggagtatgga catattcttt ttcaaccagc tgatcgatga ttttgtcgcc
    46381 cattccgtca acatccatcg cacggcggga aacaaagtgt ttcagcgact ctttacgctg
    46441 cgcaccgcaa atcaggccac cggtacagcg ggcaaccgct tcaccttcca cacgctcaac
    46501 gtcagaacca cataccggac aatgcgtcgg gaatacaacc tcacgggtat cttccgggcg
    46561 ttcagaaagc acgacgttaa ccacctgcgg gatcacgtcg ccagcgcggc gaatcaccac
    46621 tttatcgcca atgcgtaaac caagacgttc gatttcatcc gcattgtgta aggttgcgtt
    46681 actcaccagc acgccagcaa catggacagg ttccagacgc gcaacaggcg taatcgcgcc
    46741 agtacgccca acctgaaact cgacgtcacg cacaaaggtc atctgctcct gcgccgggaa
    46801 tttaaacgca accgcccagc gcggggcacg cgcgacaaag ccaagctgct cctgctgttc
    46861 cagtgagttg accttaatca ccacgccgtc gatatcaaag cccagcgtcg ggcggtcttc
    46921 ttccactttg tgatagaacg ccagcacttc ttccgccgat tcacaaagcg ttacccgatc
    46981 gctgaccggc accccccact ttttaaattg cagtaaacgg ccaagatgag tatccggcag
    47041 ctcgccacct tccagaacac caacgccata gcagaaaaaa gtgagcggtc gcttcgctgt
    47101 aatacgcgga tcaagctgac gcagtgaacc agctgccgca ttacgtgggt tagcaaaaac
    47161 tttcccgccc gtgcgtcgcg catcttcgtt aatcttttcg aaccccgcct gcggcaggaa
    47221 cacttcacca cgcacttcca gacgcgccgg gatattctct ccgtgcagct tcagcggaat
    47281 ggcgcgaata gtacgcacat tagacgtgat atcttccccg gtggtgccat cgccacgggt
    47341 cgcggcactg actaaaacgc cattttcata cagaatactg acggcaaggc catccagctt
    47401 cagctcacag caccaggtga ctttctcgtt gtttttcaga cggtcctgca cacgtttgtt
    47461 gaaagcaaga aagctttctt catcaaaaac gttatccagt gacagcattg gtacttcatg
    47521 gcgtatctgg ctgaaagctg ccagcggcgc agcacctaca cgttgggtag gcgaatcagg
    47581 cgtaatcagt tctggatgtt tggtttccag ctcgcgcagt tcgcgcatca gcctgtcgta
    47641 ttcagcgtcg ggaatttccg gcgcatccat cacatgataa agatattcat gatggcaaag
    47701 cgtcgttcgc agttctgtca gttgttgttc gattgattcc atatcgcacc atcaatgcta
    47761 aaaacccccg acaagcgggg gttcgaagag gagttaattt gccttaagtg tatcaggcgt
    47821 tggcgtcttt gacttcgcgg atgatgtcct ggtactcgcg caatttctgc ggagtcatca
    47881 tacggcgctg atcgtcaagc acgacaccgc ccacttcatc ggcaatatgc tgcgcagatt
    47941 gcagcatcag cttgaagttc tgcagctcgt caccgtaaga cggtacctgc ataaagatag
    48001 tgacacccgg agtagtgaaa tccttcattt caggatcaaa ggttcccggt ttcaccatat
    48061 tcgccaggct gaataacgcc gggccgctgc catccgggct aagatgacga tggtaaatat
    48121 tcatatcgcc aaaaatgaag cccgcttgtt gaatgctgtt aagaagcagt tcaccgttta
    48181 gctcgctacc gtgatgcgcc gcgacgttca tgataatcac cgcttctttg cgcttcggtt
    48241 tatccataac tggagccggt tccgctacag gctcaggctg tggtgccgct acgggttctg
    48301 caggctggaa agcctgttgt gccggttgcg gtgctgaatg cacaggctgc ggcgctggcg
    48361 cgacctgtgg cgaaactggc tgctgcaacg gctgttcagg ctgcggctga taggcaggct
    48421 gctgcaccgg ctgcgctgga cgcggagcat gttgcggcgg tacctgcgct tcaggcggct
    48481 gctggaccgg ttgacgcggc tgcgcagacg cataaggcgg taggtactgg tgttgcggcg
    48541 acggacgagc agcctcatgc tcctgagcgt tagccggggc atgattcacg cggtgaacac
    48601 gaacctcacc aacgccctca tcatcttcga catcctcgtc ataagaatcg tcgtcacgtt
    48661 ttgacttcat tcgttttaat ggccgatcgc ggaacataga agatcgttct ttacggctgg
    48721 tccagaaacc atgtaccagt aaagcgatta tggcgatcgc gccaacaatg attaatatca
    48781 gacgcaaatc ctgcatcatt atattctctg ttgttctaac accttgccac cacggcaaac
    48841 atttactcac taagagtatt tgccgattac ctcaagtgca agtgcactat taactttcac
    48901 agcacaaaga tagatgaaat cgtgcttttt gctgtttttt cgaacatatc ctaactgtcc
    48961 attgcgcaat tacccggtaa aatacgcaga attttcctgg gattggtcaa aaggagctca
    49021 tcctgattat ggtttcatca ttcacatctg ccccacgcag cggtttttac tattttgcgc
    49081 aaggctggaa gctcgtctcg caacctggga ttcggcgttt cgttatttta ccgctgctgg
    49141 tcaatatttt gttgatgggg ggcgcattct ggtggctctt tacacagctc gatgtctgga
    49201 tcccgacttt catgagttac gttccggact ggctacaatg gctgagttat ctgttgtggc
    49261 ctctggctgt catctctgtg ctgttagtgt ttggctattt cttctccacg attgctaact
    49321 ggattgccgc tccgtttaac ggtttattgg ctgaacaact ggaagcacga ttgactggcg
    49381 ctacaccgcc agataccggg attttcggta tcatgaaaga tgtgccgcga atcatgaaac
    49441 gcgaatggca aaaatttgtc tggtatctgc cgcgcgcaat tgtattacta attctttact
    49501 tcatccctgg tattgggcaa accgtcgcgc cggtactgtg gttcctgttt agcgcctgga
    49561 tgttagccat ccagtattgc gattacccct tcgataacca caaagtgccg tttaaagaga
    49621 tgcgcaccgc cctgcgcaca cgcaaaatca ccaatatgca gtttggtgct ttaaccagcc
    49681 tgtttacgat gatcccgctg cttaatctgt tcatcatgcc cgttgccgtt tgtggcgcga
    49741 cggcgatgtg ggtcgattgc tatcgcgata aacacgcgat gtggcggtaa caatctaccg
    49801 gttattttgt aaaccgtttg tgtgaaacag gggtggctta tgccgcccct tattccatct
    49861 tgcatgtcat tatttccctt ctgtatatag atatgctaaa tccttacttc cgcatattct
    49921 ctgagcgggt atgctacctg ttgtatccca atttcataca gttaaggaca ggccatgagt
    49981 aagatttttg aagataactc gctgactatc ggtcacacgc cgctggttcg cctgaatcgc
    50041 atcggtaacg gacgcattct ggcgaaggtg gaatctcgta accccagctt cagcgttaag
    50101 tgccgtatcg gtgccaacat gatttgggat gccgaaaagc gcggcgtgct gaaaccaggc
    50161 gttgaactgg ttgaaccgac cagcggtaat accgggattg cactggccta tgtagctgcc
    50221 gctcgcggtt acaaactcac cctgaccatg ccagaaacca tgagtattga acgccgcaag
    50281 ctgctgaaag cgttaggtgc aaacctggtg ctgacggaag gtgctaaagg catgaaaggc
    50341 gcaatccaaa aagcagaaga aattgtcgcc agcaatccag agaaatacct gctgctgcaa
    50401 caattcagca atccggcaaa ccctgaaatt cacgaaaaaa ccaccggtcc ggagatctgg
    50461 gaagataccg acggtcaggt tgatgtattt attgctggcg ttgggactgg cggtacgctg
    50521 actggcgtca gccgctacat taaaggcacc aaaggcaaga ccgatcttat ctctgtcgcc
    50581 gttgagccaa ccgattctcc agttatcgcc caggtgctgg caggtgaaga gattaaacct
    50641 ggcccgcata aaattcaggg tattggcgct ggttttatcc cggctaacct cgatctcaag
    50701 ctggtcgata aagtcattgg catcaccaat gaagaagcca tttctaccgc ccgtcgtcta
    50761 atggaagaag aaggtattct tgcaggtatc tcttctggag cagctgttgc cgcagcgttg
    50821 aaactacaag aagatgaaag ctttaccaac aagaatattg tggttattct accatcatcg
    50881 ggtgagcgtt atttaagcac cgcattgttt gccgatctct tcactgagaa agaattgcaa
    50941 cagtaatgcc agcttgttaa aaatgcgtaa aaaagcacct ttttaggtgc ttttttgtgg
    51001 cctgcttcaa actttcgccc ctcctggcat tgattcagcc tgtcggaact ggtatttaac
    51061 cagactaatt attttgatgc gcgaaattaa tcgttacagg aaaagccaaa gctgaatcga
    51121 ttttatgatt tggttcaatt cttcctttag cggcataatg tttaatgacg tacgaaacgt
    51181 cagcggtcaa cacccgccag caatggactg tattgcgctc ttcgtgcgtc gcgtctgtta
    51241 aaaactggcg ctaacaatac aggctaaagt cgaaccgcca ggctagactt tagttccaca
    51301 acactaaacc tataagttgg ggaaatacaa tgttccagca agaagttacc attaccgctc
    51361 cgaacggtct gcacacccgc cctgctgccc agtttgtaaa agaagctaag ggcttcactt
    51421 ctgaaattac tgtgacttcc aacggcaaaa gcgccagcgc gaaaagcctg tttaaactgc
    51481 agactctggg cctgactcaa ggtaccgttg tgactatctc cgcagaaggc gaagacgagc
    51541 agaaagcggt tgaacatctg gttaaactga tggcggaact cgagtaattt cccgggttct
    51601 tttaaaaatc agtcacaagt aaggtagggt tatgatttca ggcattttag catccccggg
    51661 tatcgctttc ggtaaagctc tgcttctgaa agaagacgaa attgtcattg accggaaaaa
    51721 aatttctgcc gaccaggttg atcaggaagt tgaacgtttt ctgagcggtc gtgccaaggc
    51781 atcagcccag ctggaaacga tcaaaacgaa agctggtgaa acgttcggtg aagaaaaaga
    51841 agccatcttt gaagggcata ttatgctgct cgaagatgag gagctggagc aggaaatcat
    51901 agccctgatt aaagataaac acatgacagc tgacgcagct gctcatgaag ttatcgaagg
    51961 tcaggcttct gccctggaag agctggatga tgaatacctg aaagaacgtg cggctgacgt
    52021 acgtgatatc ggtaagcgcc tgctgcgcaa catcctgggc ctgaagatta tcgacctgag
    52081 cgccattcag gatgaagtca ttctggttgc cgctgacctg acgccgtccg aaaccgcaca
    52141 gctgaacctg aagaaggtgc tgggtttcat caccgacgcg ggtggccgta cttcccacac
    52201 ctctatcatg gcgcgttctc tggaactgcc tgctatcgtg ggtaccggta gcgtcacctc
    52261 tcaggtgaaa aatgacgact atctgattct ggatgccgta aataatcagg tttacgtcaa
    52321 tccaaccaac gaagttattg ataaaatgcg cgctgttcag gagcaagtgg cttctgaaaa
    52381 agcagagctt gctaaactga aagatctgcc agctattacg ctggacggtc accaggtaga
    52441 agtatgcgct aacattggta cggttcgtga cgttgaaggt gcagagcgta acggcgctga
    52501 aggcgttggt ctgtatcgta ctgagttcct gttcatggac cgcgacgcgc tgcccactga
    52561 agaagaacag tttgctgctt acaaagcagt ggctgaagcg tgtggctcgc aggcggttat
    52621 cgttcgtacc atggacatcg gcggcgacaa agagctgcca tacatgaact tcccgaaaga
    52681 agagaacccg ttcctcggct ggcgcgctat ccgtatcgcg atggatcgta aagagatcct
    52741 gcgcgatcag ctccgcgcta tcctgcgtgc ctcggctttc ggtaaattgc gcattatgtt
    52801 cccgatgatc atctctgttg aagaagtgcg tgcactgcgc aaagagatcg aaatctacaa
    52861 acaggaactg cgcgacgaag gtaaagcgtt tgacgagtca attgaaatcg gcgtaatggt
    52921 ggaaacaccg gctgccgcaa caattgcacg tcatttagcc aaagaagttg atttctttag
    52981 tatcggcacc aatgatttaa cgcagtacac tctggcagtt gaccgtggta atgatatgat
    53041 ttcacacctt taccagccaa tgtcaccgtc cgtgctgaac ttgatcaagc aagttattga
    53101 tgcttctcat gctgaaggca aatggactgg catgtgtggt gagcttgctg gcgatgaacg
    53161 tgctacactt ctgttgctgg ggatgggtct ggacgaattc tctatgagcg ccatttctat
    53221 cccgcgcatt aagaagatta tccgtaacac gaacttcgaa gatgcgaagg tgttagcaga
    53281 gcaggctctt gctcaaccga caacggacga gttaatgacg ctggttaaca agttcattga
    53341 agaaaaaaca atctgctaat ccacgagatg cggcccaatt tactgcttag gagaagatca
    53401 tgggtttgtt cgataaactg aaatctctgg tttccgacga caaaaaggat accggaacta
    53461 ttgagatcat tgctccgctc tctggcgaga tcgtcaatat cgaagacgtg ccggatgtcg
    53521 tttttgcgga aaaaatcgtt ggtgatggta ttgctatcaa accaacgggt aacaaaatgg
    53581 tcgcgcctgt agacggtacc attggtaaaa tctttgaaac caatcacgcg ttctctatcg
    53641 aatctgatag cggcgttgaa ctgttcgtcc acttcggtat cgacaccgtt gaactgaaag
    53701 gcgaaggctt caagcgtatt gctgaagaag gtcagcgcgt gaaagttggc gatactgtca
    53761 ttgaatttga tctgccgctg ctggaagaga aagccaagtc taccctgact ccggttgtta
    53821 tctccaacat ggacgaaatc aaagaactga tcaaactgtc cggtagcgta accgtgggtg
    53881 aaaccccggt tatccgcatc aagaagtaat tcttgccgca gtgaaaaatg gcgcccatcg
    53941 gcgccatttt ttatgcttcc gccagcggcg gcaaaatcaa ttcatcgctc tcatgctgct
    54001 gggtgtagcg catcacttcc agtacgcgca accccgcacg gtgcaccgca tcgtttaacg
    54061 ccttcccttt cagcaaacca ctgataagct gagcacaaaa caggtcgcca gtccctttca
    54121 ggtcggtttt tacccgtgaa tgggaaatga cattcacgct gtcggcactg accaccacca
    54181 cctgcatctc ctgattttct tcattaccgg aggcgctggt aatcaccacc cattttaatg
    54241 tgtctgaaag cagacttttt gcggcagcaa tggcactgtc gagatcgcgg caatttttac
    54301 cggtcaggat ttccagctca aagatattgg gggtaattcc ctgcgccagc ggcagtaaat
    54361 attgtcgata cgcctcggga aggtcaggtt tgacataaat tccgctatca atatcgccaa
    54421 tcaccggatc gaccatgatc aataggtcag gatggtcttt gcgtagcgca gtcagccact
    54481 cggcaaggat tttgatttgc gatgccgttc ccatatagcc cgtggttaca gcacgaagtt
    54541 ggcgcagcgc atcacgctcc tgaagcgcac gcaaatagcc gctaaaccat tcgtccggaa
    54601 tcgcaccacc gtagaaagtg tcataatgcg gcgtattgct cagcaatacc gtcggcacgg
    54661 caaagacatt caagccgttc tgttttatag caggcaccgc aatgctgttg cctacgcttc
    54721 cgtacactac ctgcgactgc acggcgacga tatccgcctg cagcgcccta ctcttatcgt
    54781 taaacaacaa caaactactc atttaatttt ttctccttgc cgatgatcct catcgtattc
    54841 caaccgaaac tttacctgat tctggcagtc aaatcggcta ttacaaaaca aagataaggt
    54901 aattcagtga agaaaatcat ttgtctggcc attacgctgc taatgacact ccccgcttac
    54961 gcgaagttaa ctgttcatga agaagcccgc atcaacgcca tgctggaggg attagcacag
    55021 aaaaaggatt tgatatttgt gcgcaacggt gatgaacata cctgcgatga agcggtttcc
    55081 cacctgcgtc tgaagctcgg caatacccgt aaccgcattg acactgccga gcagtttatt
    55141 gataaggtag cttcgtcgtc atcgattacc gggaagccgt atatcgtgaa gatccccggt
    55201 aagagcgatg agaacgcaca gcctttttta catgcgttaa ttgcgcagac ggataaaacg
    55261 gtgcctgcgg aaggaaatta atccgctttg gtaaggcatt tacaggaggt aacatgaaaa
    55321 aatgctttat ttatcacgat gaaaaatcga ataaattttg gtggatagat tacgaagggg
    55381 atagtttagc tgtcaactat ggcaaggtag gtagtattgg taaattccat acaaaagaat
    55441 tcgataatga agaacagtgt ctgaaagaag ccagtaaatt gattgccgca aaaatgaaaa
    55501 aaggatatca agaaactcca gactttaact tcatggatct ctactatttt gatgatgaag
    55561 agattgggtt acatcttaaa acgtcacacc caaacttcca gtgccatttt actgatccac
    55621 tttatatgtg ttgctgggat gaagaatctc cttttggtag cgatgaaggt gctgatgctc
    55681 taaacgaact tgaaaatagc ctccgttctg gactgtgctg atttccctca aatgttaatt
    55741 gaaactatgt ggggtatgaa atacatcgct atggacagta ttcttgaaga ggatgtgcgt
    55801 gcgcaattac tagccgatga aatgagctct atccagagca atatgattac ctacgcgact
    55861 gcattcggtc agatcaaagt catgggtaaa atctcccata aacttaaaaa gatgggactc
    55921 aatgcactag cgcgtcatca gcttaccgca aaaattcttc aatggggtga cggtcaggac
    55981 tcaccaatac ttcaaaaaat gattgatgac cttactgctt ttccacacga aaattaaaaa
    56041 ctgcatttgt cggcagcaac aactgccgac aaatcagatt attgacaaag tgcactttgt
    56101 ttatgccgga tgcggcgtaa acgcctgatc cggcataaaa atattgctaa ttcaatatat
    56161 tgcagaaaca ttgtaggcct gataagcgga gcgcatcagg cagttttgcg tttgtcatca
    56221 gtctccaatg ctattaatcc ttaaatccct gccccctgac taaaatgctc ttccccaaac
    56281 actccggtgg agaggtagcg atcgccacga tcgcagatga tcgctaccac caccgcacca
    56341 gggttagctt ttgccacccg cagtgctcca gcaaccgcgc cgcctgagct gacgccacag
    56401 aatattcctt cccgcaccgc cagttcgcgc atggtgtttt ccgcatcgcg ctgatgaata
    56461 tccagcacct catccaccag cgcagcgttg aaaatccccg gcagatattc cgcaggccag
    56521 cggcgaatgc ccggaatact gctgccttct tccggttgca ggccaacaat ggtcaccggt
    56581 ttggattgtt cgcgcataaa gcatgaaacg ccggtgatag tgccggtcgt ccccatgctg
    56641 gagacaaaat gagtgatgcg cccgccggtt tgctgccaga tttccggccc ggtggtggtg
    56701 taatgcgcat aagggttatc gggattattg aactgatcga gcagctttcc ttcgccacga
    56761 ttcgccatct ccagcgccag atcgcgcgca ccttccatgc cctgctcttt ggtgacaaga
    56821 atcagttccg caccataagc acgcatcgcc gcacggcgtt cctggctcat gttgtcgggc
    56881 atcagcaatt tcatgcgata gcctttcagc gcggcaatca ttgccagcgc aatgccggtg
    56941 ttaccactgg tggcttcgat taagacatca cccggtttaa tttccccgcg cttttccgcc
    57001 tcgacgatca tcgaaagtgc cgcacgatct ttcaccgaac ctgccgggtt attgccttcc
    57061 agttttaacc acacttcact gccgttatcc ggccccattc gctgcaactt caccagaggc
    57121 gtattgccta ttgtttgttc taatgtactc acaatctcta tccatacgtg gtgttgcctg
    57181 atgcgacgct tgcgcgtctt atcaggccta caggttacaa accttgccat taaaaagccc
    57241 ggatcgcggg aagcgcctcc gggcgtttaa cattcactca acctatcagg cgctttgtgc
    57301 gagagcaagt tcctcatcgc gggtttcaat acgctcgtcg ccgttataca gccgcgcatg
    57361 ttgcagacca acgaataaac gctcgccatg ctgcggggca tcgtcgccat gcatcacgac
    57421 cgtcagcggt tcgttgtacc accccagcgg ctgcaccact aattgggtgt agtgaccttt
    57481 cgggctggct tccagtacct gtaccggcag cggcgaatcg agactggtac ggcggctgat
    57541 atccacttcc cacgggcgca ggaagagatc caccggcccc tgatacgcag gtgtgtaacc
    57601 cagcggccag cgatgcgcgc caacatggaa ctgcccgccg cgaatggttc cctgcaggcg
    57661 gttcacttcg cccataaatt cgagtacaaa acgggtcgct ggttcgcgcc atacctgatt
    57721 cggcgcatca gcctgttcaa tattcccctg actcatcacc actacacgat cggctacttc
    57781 ggtcgcctct tcctggtcgt gggtcacaaa cacgctggtg aatttcagct cttcatgcag
    57841 ttgacgcagc cagcgacgca gctctttacg cacctgcgca tccagcgcgc cgaacggttc
    57901 atcaagcagc agaatttgcg gttctacagc cagcgcgcgc gccagcgcca cacgctgttt
    57961 ctggccgccg gaaagctgcg ccggataacg atccgccaga tgcgcaagct ggaccatttc
    58021 cagcaatttt gtcactttcg ctttgatggc tgcggcattc gggcgctcgc gacgcggcag
    58081 caccgtcagg ccaaaagtga tattgtcgaa taccgtcata tggcggaaca gcgcgtaatg
    58141 ctggaacacg aaaccgactt tacgatcgcg cgcgtgcagg cgactcacgt cggtgccgtg
    58201 gaagcgaata tgcccgctgg tttgatgctc cagcccggcg ataatccgca gcagcgtggt
    58261 tttcccggaa ccggacggcc ccagcaacgc gaccatctga ccagaaggaa tatccagtga
    58321 gatatcgttc agcacctggg tgcgaccaaa agacttcttg atattggcaa tctcaatgct
    58381 catgatgctc ctcctgctgc gcgcgttttt cctgattctc caggcgccac tgcaacatac
    58441 tctttaaaaa cagggtgata atcgccatca acgtcaacag cgcggcggcg gtaaaggagc
    58501 cgacggtgtt gtagtcctgc tccaacaatt caatctgtaa cggcagcgaa agtgtttcgc
    58561 cgcgaatcga gccggaaacc accgacactg caccaaactc gccaatcgcg cgggcgttgg
    58621 tcagcaccac gccgtaaagc agcgcccagc gaatattcgg caatgtgacg cgacggaaca
    58681 tctgccagcc ggacgcgcca agcaaaatcg ccgcttcgtc ttcctggctg ccctggctta
    58741 acatcaccgg caccagctcg cgcaccacaa acggacacgt cacaaagatg gtgaccagca
    58801 ccattccggg ccaggagaac ataatttgca gattatgttc atccagccag ccgccgagcg
    58861 ggccgttgga gccgtagaac agcagataca ccagaccggc aaccaccggc gatacagcaa
    58921 acggaatgtc cagtagcgtc agcagtaact ggcgtcccgg gaagttaaag cgcgtcacca
    58981 gccaggccag cagaatgccg aacaccaggt ttaccggtac ggcaatcagc gcgatcatca
    59041 ccgtcagcca gatggcgtgc agcatgtccg gatcggccag attctgtaaa accggcatca
    59101 gccccttgct gaatgcctgc acgaagatgt aaatcatcgg caccagcagg atgaacgccg
    59161 aaaccagcat cccgatgcca atcagaaacc atttgcccca gttaatcggg cgcgcgtcat
    59221 aacgcttcaa ttgggtaact tccgccatta atgacctacc acacgccgac caaagcgact
    59281 ttgcagagtg ttaattgaga acagcagcag cagagatgcc gcgaggatca ccgaagcaat
    59341 cgcgctcgct gccgggtaat caaactcctg taagcgcaca aaaatcatca gcgacgtcac
    59401 ttccgtcttc cacgcgatat ttccggcgat aaaaatcacc gcgccaaatt caccaagact
    59461 acgggtaaac gacagcgcca cgcccgccac cagcgccgga gaaagctccg gcagcaccac
    59521 tttgcagaaa ctctgccagc gcgttgcacc aagcgtttcc gccgcttctt catattccgg
    59581 gcctaactct tccagcaccg gctgcacggt acgcaccaca aacggaatgc tggtaaaggc
    59641 catcgccacc gcaataccaa gccaggtata ggtgactttg atatcaaact tcgccagcca
    59701 ttcaccgtaa aaaccgttta cggaaaagag cgaggccagc gttaaaccgg cgactgccgt
    59761 tggcagcgca aagggtaaat ccatcagcgc atcaagcagc gtgcggcccg gaaagcgata
    59821 acgtgttaaa atccacgcca tcagcagacc gaaaacgccg ttaaaaatcg atgccacaaa
    59881 cgccgacagc agcgtcactt tgtaggccgc gaccacctgc gggttggtga tcacctccca
    59941 gtactgcgcc cagctcatct cagaaagctg catcaccagc gcagagagcg gcagcagcaa
    60001 tatcaggcac acaaacagca gactggtgcc aaggcttaaa gtaaagcccg gcagcacgcg
    60061 tcttgaagaa actgcaaaca ttacttacgc cccgccgcta acagcttgtc taactcgccg
    60121 ccgctggtga agtgggtttt catcacttcc ggccatgagc caaatttgtc ttccacgcgg
    60181 aacagttcgg tctgcgggaa tttatctttc agtttgtcca tgacttccgg gttattcaca
    60241 cggtagtaat agtcggtgat gatggtctgc gcctgtgggc tgtagagcca gttcaggtag
    60301 gcttttgcgg ctttttccgt accgttggcc tgcacatttt tatcgaccca cgccaccggg
    60361 aattccgcca gaatattggt tttcgggatc accacttcaa agccctgtgc ttcatactgt
    60421 ttacggatgt tgttcacttc cgattcaaag ctaatcagca catcgcccaa gccacgctcg
    60481 gcaaaagtgg tggttgcgcc acgaccgccg gtatcgaaca cttcaacgtt tttcaggaac
    60541 tgggtcataa actgttcggt tttggcttta tcgccaccgt cagctttatc cgctgcgccc
    60601 catgccgcca gataggtata acgcgcgtta cccgacgttt tcgggttcgg gaaaatcagc
    60661 ttcacgtcgg agcgcaccag gtcgttccag tcgtggatat tcttcgggtt gcccttacgc
    60721 accaggaagc ccatggtgga gtagaacggc gagctattat tcggcaggcg cgactgccag
    60781 tcagccggga tcagcttgcc tttgtcgtgc aggatttgta cgtcggtcac ctggttataa
    60841 gtgacaacgt ctgcttttaa gccctgcaaa atcgccagcg cctgttttga tgacccggca
    60901 tgagattgtt ttatcgtcag tttgtcgccg ccgttatctt ttgcccattg ctgttcaaac
    60961 ggcggattca gggcggcaaa cagctcgcgg gagacgtcat aagaactgtt cagcagttcc
    61021 gttgcctgta catggcccgc cagcagcaga gaagcgacca gcgcgagtga gttctttttc
    61081 agtaagttaa cggccattgc gcacccttat aaatttaatg actttctaat agccatcata
    61141 tttataacgg acgtgaaagg agtaacggtt ttatataccg tttggtgatt tggaagttaa
    61201 aaagggaata agaacttggt tcaacaggcg taattgttgc aggcagtttg gacacggaca
    61261 gcgcggagaa accggagcgt acacatagta cgtgaggatt tcgagcactg cccggggcca
    61321 aaatgacaaa tgaaatagcc tggtgaactt agttcaagac gaaatcctcc ccacacatgc
    61381 agggaggaaa cagaggtgaa tcagataccg acgctaaccg tctccggcag tgtgctgccg
    61441 ccatcaatca cattctgtgt accggttaaa tagctggatt catccgatgc gaggaaggcc
    61501 gccagttcgc cgacttccag cggatcggcg aggcgacaca gcgggattgc ttttgccatt
    61561 tcagtcagca ccgattctgg atcttccggg ttcgactggc gggcaatgct ttccgccatt
    61621 ggcgtgcgga cgtatcccgg gcagatggcg ttaacgcgaa taccggattg cgcgtactcc
    61681 accgccagcg atttagtcag gccaacaatc gccgctttcg ttaaggcata cgccgtttcg
    61741 ccaggatcgg ccaccatatc accagtgact gaagacatca tcacaatgcg accatctttg
    61801 cgcgcaatca tctccggcag caccgccttc gtgacgttcc atacgccttt aatattgata
    61861 tcaatatgga aatcgcgatc ttcatcgctc atatcgagga aactgcccag acgacaaacg
    61921 cctgcgttat tcaccaggat atcaatgcgc ccttcttttt ccttcgcgcg tttgatagct
    61981 gcggctaccg acgccgggtc acgcacatcg gcgacaaccg ccgtacagcg atgaccacga
    62041 ccacacagtt cgtccgctag cttttcgatc tcaggggaga tatccagcaa gattaggttc
    62101 gcgccatgac gtgcaaaagt tctggcaatt ccttcgccaa ttccctgcaa tgcgcccgta
    62161 atcagtgctg tcttgcccgt gagtttaccc attttaatgc tctccttgtt aatacagagc
    62221 gttacactcc attccctctg aaaatacagg ataaacaaaa tattgctgcg gatcagctca
    62281 ctaattcagg aaaatctcaa tccagtcgct gctttatgca tcatttcagg cgttgtgtta
    62341 gctcactgct gcgctgaatc attttcaatg acgccacatc attgatgttg cacaagacca
    62401 acaaacagta aatcggtaac agaattttgc gccgtgcggg tagacatcga cgagctacgc
    62461 cattcggttt caccggaaac ggtatcgagc gtgaaatgcg ccagccgccg cagcggagag
    62521 tcagccagag aagtaatggc aatcaccgtt gccccctgct ttcgtgccgc ttctgcgcac
    62581 aagacaatct cttttttact gccgctgtaa gagatagcga tttgtacatc gccttttttc
    62641 aacgcctggg aaacggtggc ttgtacgtgc gtgtcggctt cgcaggccac gcgatagcca
    62701 attttcatca gtttgaacga cagatcgcgc cccaccagcg ccgatccgcc caggcccgtt
    62761 atctggataa acggtgcttt actgatgacc tcaatgattt tttgcagccg cgcgtagtcg
    62821 aacaacgcac aggtttgctc cagcgccagc tctttttcgc gattcagttt gcgggcaatc
    62881 acttccagcg aatcatcact ggtaatcgaa ctgtgcaggt gcagcgccgt ggcattcgtt
    62941 ttctcccggc tggcactgta ttcgccaatt aacgccatac gcaattcagt aaatccttgc
    63001 gcgccgagct tctgggcaaa tttcacaatg ctcgactggc tgataccgag ctgtttcgcc
    63061 atctgccgtg aagagacgga tttcagctcg ctcactctgg cccgcagaaa gtcagcaatt
    63121 ttttgctcgt tcccggtaaa ttcactttcc gcattgcgta ttttcgtcag gtacaacatc
    63181 ttccactctc ctgggctggc agcaaaaatt ccgcttaagt tcttatgacg ctcttcacac
    63241 tctgcgagtg tagactcaat gattccttta agtctgtcaa ttcgggatat atgattccat
    63301 acatctgcaa ttatttgatc gtaaatagta aggtcaccac cgatgcaact agaaaagatg
    63361 attactgaag gctcgaacac cgcctcggct gaaattgacc gcgtatcgac gctggaaatg
    63421 tgccggatta tcaacgatga agataaaacc gtaccgcctg ccgttgatcg cgtcctgccg
    63481 gatatcgccg cggcgatcga tgttatccac gcccaggtca gcggcggcgg tcgtctgatt
    63541 tatctcggtg cgggaacatc cggtcgtctg gggattctgg atgccagcga atgtccgccc
    63601 acctacggcg tgaaaccggg tctggtggtt ggtttgattg ctggaggcga atatgccatt
    63661 cagcacgcgg tggaaggagc tgaagatagc cgggaaggtg gtgttaacga tctgaaaaat
    63721 attaatttaa cggcacagga tgtggtggtt ggcattgctg ccagcggtcg cacgccgtat
    63781 gtgattgccg gactggaata cgcacgccag ctcggctgcc gcacagtggg aatttcctgt
    63841 aatccgggga gcgccgtttc aaccaccgct gagtttgcca ttacaccgat tgtaggtgcc
    63901 gaagttgtta ccggttcttc gcggatgaaa gcaggtacag cgcagaaact ggtgctcaat
    63961 atgctttcca ccgggctgat gattaaatcc ggcaaagtgt tcggcaacct gatggtcgat
    64021 gtggtcgcca ccaacgaaaa actgcatgtg cgccaggtca atatcgttaa aaacgccacc
    64081 ggatgtaatg ccgaacaagc ggaagcggcg ttaattgcct gcgagcgcaa ctgtaaaacg
    64141 gccattgtga tggtgctgaa aaatctcgat gccgcagaag ctaaaaaacg cctggatcaa
    64201 cacggcggct ttattcgtca ggttttagac aaggaataac ccatggccaa agagatcagc
    64261 agtgaacttc tgaacaccat tcttacccgt gtcggcggac cgggaaatat cgccagttgt
    64321 ggtaactgta tgacgcgcct gcgtctgggt gtacatgaca gttcactggt tgatcccaat
    64381 atcaaaacgc tggaaggcgt gaagggcgtc attttgacca gcgaccaggt gcaggtcgtt
    64441 tttgggcctg gtaaagcgca tcgtgccgca aaagcgatga gcgagctgct gggcgatgca
    64501 ccggtacagg atgccgcaga aatcgccgcc cagaacaaac gtcagttaaa agccaaacaa
    64561 acctccggcg tgcaacaatt tctcgccaaa ttcgccacca tcttcacgcc gctgatcccc
    64621 ggttttattg ccgccggtct gctgctgggg atagcgacgt taattgccac ggtgatgcac
    64681 gttccggcag atgctcaggg cacgctaccc gacgcgctga attttatgaa ggtgttcagc
    64741 aaaggtttgt tcaccttcct agtgattctg gtgggctata acgccgccca ggcatttggc
    64801 ggcacgggcg tcaatggcgc aattattgcc gcactctttt tgctcggtta taaccccgct
    64861 gcaaccaccg gttactacgc cggttttcac gatttctttg gtctgcccat cgatccgcgc
    64921 ggcaatatta tcggcgtgct gattgccgcg tgggcctgcg cccgcattga aggcatggtg
    64981 cgccgcttta tgccggacga tctcgacatg ctgctgacct cgttaatcac cctgctgatc
    65041 accgctacgc ttgcgtacct gatcattatg ccgctgggtg gctggttatt cgaaggtatg
    65101 tcatggctgt ttatgcacct gaacagtaat ccgttcggtt gtgcggtttt agccgggctg
    65161 ttcctgatcg ccgtggtatt tggcgtgcat cagggcttta ttcctgttta cctcgcgtta
    65221 atggacagcc agggattcaa cagcttattt cccatccttt caatggcagg cgcgggccag
    65281 gtgggcgcgg cgctggcact ctactggcgg gcgcaaccgc acagtgcgct acgcagtcag
    65341 gtacgcgggg cgattattcc cggcctgctg ggcgttggtg aaccgctgat ttacggtgtc
    65401 accctgcccc gcatgaagcc gtttgttacc gcctgtttag gcggcgcggc gggcggtttg
    65461 tttatcggat tgatagcctg gtggggtctg ccgatgggct taaacagcgc ctttgggccg
    65521 tctggtctgg tggcgctgcc gctgatgact tccgcacaag gcatccttcc ggcaatggcg
    65581 gtttatgctg gcgggattct ggtggcatgg gtttgcgggt ttattttcac cacgctcttt
    65641 ggctgccgta acgtcaatct ggactgatat tatgaaacgg acaatgctct atctttctct
    65701 gctggctgtt agttgtagcg tcagcgccgc aaaataccct gttctgacag aaggtagttg
    65761 atcaagtata tgcggcgtta aagcagtaat aaaaaggcca gtcagtaatg ttactgcctg
    65821 gcctttaatc aatgaatcag aaacgcttac agcgccatca acttgtccag cgacggtgcg
    65881 aaataatagc cgccggttac cggtttggtg aaacgcaaca tcgcatcacg tttgccatcg
    65941 gtatcgccaa acatgctcag cagttgctgc tcaatgttat gcagacgcgc gcagtaggcg
    66001 cagaagtaca gaccgtgagt gccactggcg gtgccgtacg gcaggctctg gcgaacaatc
    66061 ttcagccctt tgccatcttc tttcagatca acgcgggtga ggtgagaggt ttccggacgt
    66121 tcgtcgccgt caatctcttc gttggcctct ttggtgcgcc cgatcatcat ctcctgatcg
    66181 tgaacgctca tccggttgag ctgcttcagg ttgtgctccc aacgctgcac aaacacatag
    66241 ctgccgccgg aatccacgcc gtctttgata accgccactt cgcggcgcgt ctcttcaccc
    66301 gccgggtttt ctgtaccgtc aacaaagccg ctcaggtcac gctcttccac ccaacggaag
    66361 ccgtggatct cttctttcac ttcaatgcag tcaccaaagg cttccatcgc cgcctgagcg
    66421 acagagaagt ttacgtcgtg acgcagagaa agaatgtgga tcaacacatc gaactgggtc
    66481 gtcggcgcaa ggcctttacc gtagcccgga aaatctttca gctcttctgc cccaacgccg
    66541 ccgctcagag cgcgccaggt gttgttacca aaggcaacca ccgcaccaag atgcgcgtcc
    66601 gggaatttcg cttcaaaagt tgccagtttg tcggcaaatg ttttactggc cgcacgcagg
    66661 gcgtcaactt cccctttcac gttggcttcg atccaaatcg ccgcgcggca atgttctggc
    66721 aaaatgccac tctgaacctg agacattgtt cctcctgaaa ataagaatgc cacgcaatcc
    66781 gtggcattgt tgagcgctat tttacgtgtt ttttcgcgac ggcggtttgt tcagacgcaa
    66841 attaacgccg ccagataatt ttactgactt tccagttttt cagggtatcg tccgaaggca
    66901 ttaacccttc aggaccacgc cattccccgc tgaactggta gctaatatgt tgactgcctt
    66961 cggctttaca ttcgacggca cgattatcat caccatcagc tttttggcaa ttgccaaatg
    67021 ctttgctgta aaggtcgcta aacggtgtac cgattttaac accggtgtca gcaggaatat
    67081 cgctatccag cacgtcaatg cgactgatcg tgccttgatc gccgttaatc accatcgcca
    67141 cgttgtcgcc tttcatcact tcaaaaaagc gcaccacgtt gccgttcgtg gttttcattc
    67201 cgctgcgcag gcgataatcg ccatcaagcg catcagcaat ggcttgttct tgcagtggtg
    67261 tggacgccgt taattcaccc acgccctgct cgctcacttt ggtggacgat ccaaaccagt
    67321 tccacgggtt agcggcagac cagttaactg acgacatcgt ggaacagccg gtcagcatca
    67381 acggcatagc gcataacatt aaacgcagcg atttcatgtc acttcctttg gttattcaat
    67441 aacgttgctt ggagtgcaaa ttcaccaaaa agtgccgtta ttctttttct tcattaaaac
    67501 aagcgcgtaa acgtcgattg gtcagcagcc agattaacgc cacaatatcc gccactacca
    67561 gcgccagacc gataccgcta acggattcac cgttcagcca cagccacggt tgccagcaaa
    67621 gtaaaaccac ctgcgccaac agcaacagaa aatagagcac atgccaggtg cgaggaaacg
    67681 aagcccgccg accgctcaac aaaaacgcca gcaccgccgg aataccggga atcagcccca
    67741 gccagaaatt atcgtgatcg ggataaaaca gatttagcag cgcagtaccc tgctcacgcg
    67801 acgcaccggc aatgacaaac agcacccagg ttcgcgcctg aagcaatagc acaagccaga
    67861 agagcaaggg taaacgcagg cgtccgtgcg catcataatg gacaggatgg aactcagtac
    67921 tcttcatctt caatcaaacg cttacccaga ctcagcacgt cggcgtgttc atatcccagg
    67981 cgttcataca ttccgagcac catgtcgtta tcttccggca cgttgatctg aattttcggg
    68041 cagccgcgag caatcagctt tttctccagc cggttaagca acgcattggc aattccacgc
    68101 ccacgaaact ctggatgcac gccaagataa taagcagacc cgcgatgccc gtcataaccg
    68161 cccatcaccg ttccgaccac ttcaccgttt acctcagcga ccaaaaacaa actgacgtca
    68221 tggttcatct tacgctcgat gtccatttcc ggatcgttcc acggacgcag caagtcgcaa
    68281 cgctcccaaa gggtgatgac ctcttcgaaa tcttcctggc gaaatacgcg tatctccatg
    68341 gtattcgtta cctttttgcg ggttaaaagg ctgattatgg cgtgaacggt cgaattagcc
    68401 aatatctgac gaaaatcggt tgaaaaagtg gcataatggg gagttgtcaa ctattgaaat
    68461 gaaaagtaaa acaattctca acagcaaacc gtcgtaacgg attacgcgat acgatataac
    68521 atctggaact ttattattac aactcaggcc gtatgagcac ttttaaacca ctaaaaacac
    68581 tcacttcgcg ccgccaggtg ctgaaagccg gtttagctgc cctgacgttg tcaggaatgt
    68641 cgcaagccat tgccaaagac gaacctttaa aaaccagcaa cggacacagc aagccgaaag
    68701 ccaaaaaatc tggcggcaaa cgtgtcgttg ttctcgatcc aggccacggc ggaattgata
    68761 ccggagcgat cggacgcaac ggttcgaaag aaaaacatgt ggtgctggcg attgctaaaa
    68821 acgtccgttc cattttgcgt aatcatggga ttgatgcgcg tttaacgcgt tctggcgata
    68881 cgtttatccc actttacgat cgcgttgaaa tcgcccataa acatggcgca gatctgttta
    68941 tgtcaattca tgccgatggc tttaccaacc cgaaagctgc cggtgcttcg gtatttgccc
    69001 tctctaaccg tggggcaagt agcgcaatgg cgaaatacct gtctgaacgc gaaaaccgcg
    69061 ccgatgaagt tgccggtaaa aaggcgactg acaaggatca cctattgcag caagtactgt
    69121 ttgatctggt gcaaaccgat actattaaaa acagcctgac gcttggctcg catattctga
    69181 aaaagattaa gccggtgcat aaactgcaca gccgcaacac cgaacaagcc gcgtttgtgg
    69241 tgttgaaatc gccgtcggtt ccttcggtgc tggtggaaac ctcgtttatc accaacccgg
    69301 aagaagagcg gctgttaggc acggcggcgt ttcgtcagaa aatcgccaca gcgattgctg
    69361 aaggcgtgat cagttatttc cactggttcg acaaccagaa agcacattcg aaaaagcgat
    69421 aagttatgaa acccgacgca caccaggtta aacagtttct gctcaacctt caggatacga
    69481 tttgtcagca gctgaccgcc gtcgatggcg cagaatttgt cgaagatagt tggcagcgcg
    69541 aagctggcgg cggcgggcgc agtcgggtgt tgcgtaatgg tggtgttttc gaacaggcgg
    69601 gcgtcaactt ttctcacgtc cacggtgagg cgatgcctgc ttccgccacc gctcatcgcc
    69661 cggaacttgc cgggcgcagt ttcgaggcga tgggcgtttc actggtagtg catccgcata
    69721 acccgtatgt tcccaccagc cacgcgaatg tgcgtttttt tattgccgaa aaaccgggtg
    69781 cagagcccgt ttggtggttt ggcggcggct tcgatttaac ccctttctat ggttttgaag
    69841 aagacgccat tcactggcac cgcaccgccc gtgacctgtg ccagccattt ggtgaagacg
    69901 tttatccccg ttacaaaaag tggtgcgacg attacttcta cctcaaacat cgcaacgaac
    69961 agcgcggcat tggcgggctg ttctttgatg atctgaacac gccagatttc gaccactgtt
    70021 ttgcctttat gcaggcggta ggcaaaggct acaccgacgc ttatttacca attgtagagc
    70081 gacgtaaagc gatggcctac ggcgagcgcg agcgcaattt tcagctctac cgtcgcggtc
    70141 gttatgtcga gttcaatctg gtctgggatc gcggcacgct gtttggcctg caaactggcg
    70201 ggcgcaccga gtcgatcctg atgtcaatgc cgccactggt acgctgggag tatgattatc
    70261 agccaaaaga tggcagccca gaagcggcgt taagtgagtt tattaaggtc agggattggg
    70321 tgtaactccc tcacccccat tcccgcatcc gctgatgcaa cgtcaacgac ggcttctcgg
    70381 caaacagctg ctggtaatcc gtggcaaatt gccccagatg ccagaatccc cactgcatgg
    70441 cggcgtcttt taccgttgtg ctttgcgacc acgggcttat cagttcgcgg cgtacgacgt
    70501 tcaggcgaat gcgtttcagc cacgcgtttg ggccaatgcc taaaatagcg tgaaacgcgt
    70561 tttgtagcgt gcggcgactg acatgcagtt gattacacaa atccagcacc gtcaccggtt
    70621 cggacatgtt ttccagcaca tattcacggg cgcgggaaag caaccgacgg taactctgat
    70681 gactgatgct ttccgccgtc accattggtt gcgcttcttc cagcatcgcc cccatcgcca
    70741 ttagcaaatt atcccccagc acttttcgca ctgcaggctg atggagattt tccgggttct
    70801 cgcaaaacgt cgccagcgcc tgttggacaa agccccacag cgcggcttta tgctgctctt
    70861 tcacttccag cgccgactgg ctacgcagca tatgtaatac ccgatccgga ttgtgcaaaa
    70921 agttagcctg acaggtgatg acatcttcag acagcactac gcccaggatc gtataatcgt
    70981 ccggcgtact taattcaaac tcggttccac cggggcgcgt cgctatttct gcgcttccca
    71041 gacattgcga accgataaat ccctgctcac cgcgcgtcgc cggaatgcca aaccagaacg
    71101 agttcggcca gaccaggcac gactgacgca gcgccagacc ggtgtattca cgaaaaacct
    71161 gaatatcatc gagtagaatt tccgtaaatt caccatgaaa cttgcccgga tgcagctgat
    71221 cgtaaatctg ctgctaggcg gtaatcgtca aagcatgttc atagacatcc gttgtctgtc
    71281 gttgatgaac attatccact tcgaccttcg gcgtgagctt caggttttcg ggtaagggtt
    71341 catgataaag atggtgcaaa ttggctgtac gggtcttttt catgatgtta atgccgggtg
    71401 ttgtaggaca cccggcacct ccgacaggtt aatggggctt gagacgataa cgactactgc
    71461 gtttacgtaa cgtcccggca gaaaagagct gttcgagcgc atttctggct ttttccagcg
    71521 gccagccaaa gtgggcggcc acttctcctg ccgtcattcc ctgacatact gatgttaaca
    71581 gcgccaacaa ctcgtcagca gattccgcga cgataactgg cgcgtcgggt tcctttacgg
    71641 gttgcttcgg ctggcggtta aagccagtaa ccagccactg ggtgtcatcg tctggccgac
    71701 cgatctcctt acggctgatg acacgcccgg tacgcatcgc ggcagcacaa cctgcgtcca
    71761 gcgccgcacg acacgccgcc agatcgcctt ccaccaccag cgttaagcga ccagggtcaa
    71821 gcacttcgtg actgagcaga cgaacgttag ctgctttgag catggcatcc gctgcatcta
    71881 tcgcggcgac catgccgtcc acttccagca atcccagtgc attgatcatc ggcaacctcc
    71941 gttacgcacg ctggattgga ttacgcgcga tttccagcac tgcatcggta aaggcgttac
    72001 aggctgcttt acacgcggcc tggctaccgg ttaaaaatgc tgccgagtag ttggtttcag
    72061 acggtggcgg gacgtaggtc accagctgta cgtcggcaga tttcaacgcg gcatcaatgc
    72121 cgtaggtcgc ttccagcggc ggtgccacca gatacgccat cggatcgcca agcgtgatcc
    72181 cggcggttga tgaaagataa gaaccggtac gcgaaaccac atgcgccagg aaggcggtgt
    72241 tttctgcgtc attggcccac tggaaagctg cgccattttc aatatgtgca accatcgcat
    72301 ccagaccggc acgcacttcc gccgggtttg gcccaccaag cataatcagc acttcaccgg
    72361 cagtcggtga cgggccgtgt gccgctcccg catacagcga gcgaccatat accacttcga
    72421 ccatcgcttg tttggtcgct tcgtcagccg caatgtaggt tacgtcgtca gaatcggcag
    72481 aaatcagccc gaggctacga atatgcggcg gcaattttag ttcacgcgca aattcagcgt
    72541 taacggaggc aatcacccgc atggcggtta ccgacggtcg aatcaaatct aaagctggca
    72601 tgatgcctcc ttatcgggtc atgttgatgc cggatgcttt ctgctccagc atacgtttcg
    72661 ccaaatccac aatgacggca cagcagcacg aaagagaagc aaaagtcagc tacctggtac
    72721 actaggcagc gggtcagcaa aggggggcaa atgccccttt tttcatgtca gataatcaga
    72781 tcattacctc aataaactaa taatcacatc gcggttacaa ccagtttgat agtgatagct
    72841 caaacccaga cgtggcgctg gctgcaagca aaataagccg caaaaatgcg accccatgtt
    72901 tactagccgc aaaaatgcgg tcctatttgc cagaaccttt tattattcaa ttggttagtg
    72961 caagaaaaag cgtgattact gttcaagaag tatccagaaa ggtcatgatt ttgacgctga
    73021 aaaaaaatag gtgggcattt ttgcccactc actagccgca aatttgccca ctcactagcc
    73081 gcatttttgc ggcccaaagt aataaataca gagaaataat aaatacagat cagcgccaag
    73141 atttttcgtc ttttcggcgg aatcatgaga ctagtgctta cgtcctgata acctccagag
    73201 cagaccttaa cttctgtgca gcaggactac gctcattgcc gctcggctag gtcaaatcct
    73261 cacgataaag ctgttcggat tgtgaccata atgttaaagg actctttaaa tcattccttg
    73321 acgaattaac tagctggctt aagcggaata gcatgcagga taaaaacaaa accagcttag
    73381 cagtggggag agcttcgctt cgctcgtctc ctggggccaa tgctcaatga aacgacgtaa
    73441 ggcacggagc gctcaccata ggtagacaca taaacatctg agagcgcacc ttctatcgcg
    73501 ctacgcgctc aataaaaacc aacgacaccg ccacactgca agaccaatgg tttttatcgc
    73561 gtatacagac cagacaggaa acagcagcaa agcagcgtat agccaggtgc agacagcaca
    73621 ctcacacgac gcacagcagg cttaagtgat cactcaggaa actttatccc cgccgccgat
    73681 caccgcgaac tatcatgacc gcgccaaatt cgaaaggaat ggcccataga acgcgatgaa
    73741 cacacataag cgctacactt acttaacgtc gccacagagc gattgagagc gcttaaaatc
    73801 gatgcgattt cattaaattt ttctcactcg cctgaatggt ggcgatcagg actgatcgca
    73861 acattgcccc gcctcgatat tgagcgcata atagatacat acattaagcg gaaaagagat
    73921 aagtttattg tgaaaatcag ggggttacat atcatgcgca tatcagaaca acaacggcag
    73981 atcctcgact atctggcaaa aaacgaatca atcaccgcac gacaagccgc agatctcgtt
    74041 tatggtggca atgttacccg cgtccaggtt gacgccgccc gccgctcact catgacgatg
    74101 gttagtaatg ggctaatcag gaaacaaggc cgcgctttcg tagctgaaag acatatcaat
    74161 cacgcttata gccaggacgt tgaagaattg cgccgccaga tcctttgggt attcagcaaa
    74221 cagggtcagg agtatttcct ttctgaacac ccaaatttcg ccgccaaaga gttaacagta
    74281 agcggattgt cgtgcgcact ggattatctt gtcggtgaag gttgcgttac ttacaagcag
    74341 ctatggcacg ccctgaatac gcttgtagaa gatggtttac tcattgccac gcgtgagaac
    74401 atcgaatact ggataatgcc gcgttcaggt cgccgcaatc atgcacggta tatttcaggc
    74461 aacagataca gaacgccgga ctttaatccg acacaggaag atcgggaccg tgccgaaatg
    74521 atgtgcaggg aacaggagaa aagaattatg gataagttat tttcgatgca gtgaacacaa
    74581 gcgggcgcaa tgcccgtttt tattgcctta ttccacgcct gacgaatcaa tttgatcaca
    74641 cctgagaaaa tgcatagcca gaagttaagg ccgttatatt gcgattggga gcgttttaaa
    74701 cggtatcaat ttaattaatt ctgatgtggt gatttgcagg caaaaaaaac ccgccggaga
    74761 agcgggaaat agagacatat cgttgtagtt attaacagat cactttgagg tcgagggcgg
    74821 ctaaccctcg ctaatattat cccgctatta ccttgcgggt gcaaataatc ttttattgct
    74881 ttgatgattt aataaatagc actttttgct aacaaaaacc gctgtcaata tatggccaaa
    74941 aatgtcaaca gaaaaaataa gatttataat attaagtatc acatgggtaa tgggttcagg
    75001 gaatcggaat ccaccgcctc acggaaattc aaaatttgct tcttctttcc tccgtgggcg
    75061 gcttcttttt cgtatcgagg tatttatgaa agcaataaca ttagaacagt tcgcagcaat
    75121 ggcgaatgta tcgaccagaa cgctgaggcg ctggctaaaa aaaccatcaa ccggagtccc
    75181 tttgccattt accccacgcg gtactcgcca attattctgg gagcacgaat gcaaagaatg
    75241 gttgtacaga acagaacgat aataatcacg catcacattt tgaggtaaac atcatgacca
    75301 atatcgaaag attagaacgc tgtgccgata ttatgcgacg cagatggatt tatgaccctg
    75361 aagcgggttt attgtttagc cgtgaaacaa aagacgttat caaaggcagc atgaccaata
    75421 aagggcattt ggttgtaacg gtacatgaag gtaaattcaa agtgacactc acctatcaga
    75481 aagcctgcta tgtatacaca tacggcgctt atgacgagac attatatgaa gtgcaccacg
    75541 ttaatttgaa caaacaggat aaccgcatca agaatattcg cctgatgccg aaagaaaagc
    75601 accgccggat acataaaaac gtccgcaaac ttattcggct gggccacaca caatggcttc
    75661 aaccgcaatt aattgaacaa tcactataaa cacaggagta aataccatgc attcattaga
    75721 cagttatttt cagcgcacga cagcccctaa atcagccgcc caggaacgcc gtgaggaatt
    75781 ccatgagaaa gtgatgcgtt ctgctgatta tattgcggac aagttcgtgg aaacggttcg
    75841 tccacttgtt gatgaagtag ctgataaact tcagtctgaa atgccggaag atatggaagg
    75901 cactgcaaag cggcgactca tttgcgaatt aagtcgtcgt tttggcgtgt caataagcgc
    75961 ttttaaataa tcgaaaaaag gggggaaaga taatggacgc atttgaggta ctggcaaata
    76021 aaggcgctga actggtagca cagcgtgata aaaccgccaa cgaaggggaa cgcgcactgc
    76081 taaataaaca gattaaagcg atccgtatgg cccagtttaa attaatcagt aatgaagtta
    76141 tcgaaacaat taaaccgcag atcccacaaa tcgttgctga cgctattaaa gccgccggac
    76201 tggagaaacg cattgccggg ctaaaaacga acgcagagaa aatggagatg tacaaacaga
    76261 gcggtgctcc gaatcccgat gagtacttct caccggatgt tgagtttatg gcgcaggttg
    76321 aggaacgtct gggtagctgt ctcaccgagg aacagcgtcg ctattttgat ggagtggata
    76381 gttcggcagg tattgacctg aactcgtatt tcggtcgtga aattgagcat tttgatgccg
    76441 ccccgtctat gccggagcca gaaccagaaa tggccaccga tcctgaagct gagcaacgga
    76501 aacgcgtatt caatgcgata gggattcggt actgatcgga taaaagaaag ccgggaatta
    76561 ccccggctga cttaacaatg tgaccaatga acaaatcgga caagacaaac aggataagca
    76621 gtctcgaaac aatgttcggg aactatatta gcacaagtcc ggagaaaatc atcatgttgg
    76681 cactacgcat catgcagggc atcgctaaga ccctcgcaga acatgtctta gacctgaaac
    76741 attcgccact gagcaaacag gccatgaagc gccaaacgct tcgtctgtgg gctgaatatt
    76801 cgcttggaac tatcaataaa atcatcgaca tgaaatcggg gccgagcaac cagagcgcgg
    76861 aggaaatgga gttcatcagg cgtttaattc tcattcgcag agacattcat agccagttac
    76921 acagcgtggg aatcgatatt aatgatggga cgggcgatta attttcggac aagtactatg
    76981 cgcgcgcgag gagctgaaca tgaagcaaga tattgcagac aggctggaga tactcgaagg
    77041 ccagcgggca gaagcgaaac aactacgtaa gcaggccaga agagcgcaca ggaacaacga
    77101 agcagagtta ctaaccaaat acatcagttt caccaattac tgcatctacg agtgttacaa
    77161 ggaagatgca gaggactggc tggattcatt gccagaacag tactgagcag aataacacca
    77221 cgtcatgttg ttctgaggtg agcatatcac ctctgttcag gtggccaaat ttagtgtgcc
    77281 acttcagcat caccgttgaa atcacaataa cgattttttt gtaaatatgc tctcacatat
    77341 gtaggaatag accaggttgg cattgaaaca catctaccat gaccataata aagtaatttt
    77401 ttgatatgtt tatggtttta tattaattaa aatatatcta aatttgatag ttttaaaatt
    77461 gcaaaatcca gattgtgttt gtaatgttta atctgattgt attgagtggc gtttattatg
    77521 aaaaataaat caggtaattc ttgttataga gaagcagtcg atattcatag ctcacttgag
    77581 ctttcattta tattgcataa atatcatttg cgtgtagatg agaagaaatc tcgaaatgta
    77641 ttcagcgcca tacgtgatga gctcatcctt gatggggatt cacgacaaaa cttagctact
    77701 ttctgtcaaa catggcttga tgatgaaatc cgtgagttgc ttaatttagc tattgataaa
    77761 aacatgattt ataaagatga atatccacaa atggcggaac ctgaaggtcg ctgtgtgcgt
    77821 atgctttccg attcgtggag tttccccgat tcgcgacaca ccttaggttg ctcaaccatt
    77881 ggttcatctg aagctgctat gctcggccgc ttggcgttaa aatggcagtg gtgtaaaaaa
    77941 cgcgaagtac aaggtaaatc aaccgaacct gatttgcgat cctgtgcaaa tatgctggca
    78001 taaattctgc cgttactgtg atgtgaaatt gtgtgaaatt ccacttgatg atcatcacct
    78061 gataatgaat gctaaagagg ttttgaagca tgtcgatgaa aacactattg gtgtcgttac
    78121 gactttaggt attacttatt tttgtaaagc agattgcgtt agtgttttta agataagtgt
    78181 taaagttaat attagttttt atatttacat cgtaattata tgtttctatt ttaaaaatgt
    78241 tcgagagtat aaaaatgtct aatgctaata tcgcacccaa aagtaagaaa atgacagtgg
    78301 ggacactcgc tatcatgaat ataactgctg tagtaagtct acgatttata ccttctgaag
    78361 cagaatacgg cttaggtgcc attttttact atacctttgc tgcaattatg tttttagtac
    78421 ctgttgcctt tgttgcggct gagctagcta caacatatcc acaaaaaggg ggggcatttc
    78481 gatggatagg agaagcattt ggggctcgct ggggttttgt aggaatgctt atgacctggc
    78541 tacaagtcat cccctacttt ccaacagtac tgacatttgg tgcagtctcg gttgcattta
    78601 ttgatgttga tatgggggtg gcagaatcaa ttgccagtaa taaatggtat attttatttt
    78661 tcgtactatt tgtttactgg ggagctattt gtgttgcatt acgtggcgtt ggtattttct
    78721 cgcaggtatc taagtggtgc ggtattatag ggacaattat tccagctatt atccttgtta
    78781 ttcttggttt ctcctatctg tggttcagcg gtaaaccagc ggtgatccca ttaggttggg
    78841 gtgatctttt ccctaatata gctaacttcc agaatattgt tcttgccgcc agcatatttc
    78901 ttgcttatgc agggatggaa atgaatgcgg tccatgttaa cgatttagat aatccaacta
    78961 aaaaataccc aattgcaatt accatcgctt ctataggtac tattgcaata ttcctgctca
    79021 gtactttagg ggtcgccttt attattccta cagataaaat tagtctaact caaagtttgt
    79081 tggttgccta cgatatgctg ttcgaatggg ctggagtgcc atggcttggc tctgtgatgg
    79141 catttatgct agcaattggc gtactgggtg gggtggttac ttggattgca gggcctaata
    79201 caggtgtatt agcgatcgcg aaagctggtt atttacctaa gtttttccaa aaaaccaatc
    79261 gtcatggaat ggggcaccat ctaatgtttg tacaaggtat aattgtctca gttctttctg
    79321 ttacttttgt gataatgcct tcagttcaag cagcttttca gattttatca cagttaacag
    79381 ttatgctcta tttagttatg tatatgctca tgttcgctag tgctatctat ctacgacatt
    79441 ctcaacctga tacaaaacgc ccatatcgag cccctgcttt atttttctgg gcaacacttg
    79501 gattcttagg gtcttttctg gcttttgtat taagttttat tccgcccgag caaatcccaa
    79561 ctggtagtcc aaccagctat gttctttatt tgttagtatt agttgcccta tttttattaa
    79621 ttccaatatt aatttacgcc ttccgaaaat ctgactggaa agatccaaac tctgatttcg
    79681 aaccattttc ttgggaaaaa agaactagta attaaatttt ccataagcta caaaagatga
    79741 ggttaaatat gtttagtgct gaatctccaa ttggtgtaat agactcaggt gtcggtggat
    79801 tgacatcggt aaaggaaata attaatttgc ttcctggtga agatattatc tattgtggag
    79861 ataatatgaa tgcaccttat ggcaaccgtt ctgccgatga tatcatcagt ttgactaaaa
    79921 aaatgctgac ttttttacag tcaaggaatg tcaaactagt tgctgtcgca tgtaatacta
    79981 tatcctcaac tttggaatcc gaagaataca gcggatatgc taagagcttt ccgtttccta
    80041 tcttatccat cattgaacct gcagtagaag atgtgattcg ccagcaatat aaaaatgttg
    80101 gtattatcgc taccgagttt actattaaaa cggggtgtca caaagagcta ataaaaaaat
    80161 tgaattcaac aataaatgta tttggagaac caagcaaaaa tcttgcaatg ttaattgagg
    80221 aaggtaatct taatgctcct gcaatcttga atgacatcaa aaaacatgtt aaccatttaa
    80281 tttccctcca tccagtaaat gaaattattc tcggctgtac tcactatcca attgttcaga
    80341 atttttttga agcagttgca ccggatataa aatttattaa tcctgcacat gatcaggcta
    80401 tctctattaa aaatcacttg ggacagctaa atttattaaa ctcctcaaat ataggaactt
    80461 tgcaaataaa tactagtgga agtatggaga tctacaaaac ggtactaggg gaattgagca
    80521 ttacgaaacc tcatacattc agtatcaggc agttttaaat ttatccggta caccgtaaaa
    80581 tcccgtcctt ctgaactgca ccccaaaagt tggacaccaa ctgagtaagg tgcattttat
    80641 ggacaaaccc agttacatac tgggtagtgc atccaattag tagaacatgt gtttttcgat
    80701 aaacgctccg atcacttttt cgtggatctc aactgagcgt gagaagcaga ttgttttacg
    80761 acccaaccgc ttaatgcggg tgcgtagcgt cagattatta cgctcaatgc gttgggtgaa
    80821 tattttgccg gtcagatgct tattcttcgg cacctcccgg ccatagctgc cccagtcatc
    80881 gctggtcagc atgccgatgt tgaagggtgt aagcagtgcc agtagctccc ggcacgtttg
    80941 atcggttcgg ggaccaaaag tgtaggccag tacaccgcct gttttggtgt tgtacgcgta
    81001 cccgagccaa tgttgccggg ctttactgcc aacgtagctc cattgctcat caagctcgca
    81061 gataaacgcc acatcagcat gggcaacagg cgaagaagtt attcgctttg gcgcgagttt
    81121 tttaaagtcc ggatgacggt gttactacca attttcagtg tcctggcggt atcgcgaacc
    81181 ccggcaccat taaaggccat ttcagttatc agctctttca tacccggctt acgtgcttga
    81241 taagtataag tgaactgaaa cacacggtgg cagtcacggc agcgaagtct gtcacggcct
    81301 ttagggttct gaccatggcg gtaaacctga gctgactgac aacggggaca atgaatatta
    81361 acgctggtca tgagataacc tcaaaagccc gtattataca tcagattcaa ctaattagag
    81421 gcatcaccca tccagcatta taaaacatac taacaataac taaattataa taatcacaac
    81481 ttacaatcaa ataataaatg cataagatta acaatgattt tttcgggtta tatttatcta
    81541 aaaaatcaga acattatgta acttattatt ttctaagcga aaatttattg tgcggtcaga
    81601 ctaacaaact tgcgtaccac ccgaagaaca tgcgaagatt taagtattct ttcttttttg
    81661 gtttttattt ttcattttct cttttcttct ctttccggtt ctgttctggt tttggttttt
    81721 tcctttttat ttttattctt tcttcagggt tatgctaaaa caccaaaaca tttttcacca
    81781 caaaagcatc caatcatctt gccagaccaa tcaccttaac cggatacact gccggatata
    81841 atcccactaa cacccagaca ccaagcacca tacctgataa ctaccgtatt caggaaggaa
    81901 atgcgagtga tttcaccgtc accggcacaa ccgatccgcc aaaaagaggc gtaccaatgt
    81961 cgatatagtc ccccgcgcgg acaatcactt cgtcaatgac tgccaacggg agttgttgta
    82021 gctgtgggcg caacaacatg cccagagctt taccaaagtc ctgcccggcc accaccagca
    82081 ggggatgcgg attcggaaaa cgcgcgacga aatcgaccag cgcgttgatg accgtcagaa
    82141 ccgcggcgta acgcacaggc agcgaggcgg gaagcgccag cacgtacacg tcagttttgg
    82201 gatcaagatc cagctgaagc agcgcctgtt gccaggcact caccagatcc gtttcatcaa
    82261 tcgggatcgc caccggcaaa ttgcgcagcg gcagttgtac gccctccagc cagattgtgc
    82321 tgccagagag cgaaagggta tgtgcacccg cgccaatcac cgtggagcgt acggtttgcg
    82381 ccggaaactg cacattcatc tcacgcaggc gcggatggtc atgcagcgcc gttgccagaa
    82441 gtgggccaat atcggcaaaa cagaacgggt tggcgggctg gtggcgataa cattcgccca
    82501 cgcctccaga aagcgtaatg atttcgggcg taacacctgc gggcagcaaa ccggtttgca
    82561 tcaatgcctg cgcgagcggc gaaagcgttc cgtcaatcac ttcgacaatc agcgccgcca
    82621 tccgccgggt aacctgcacc agctgcgcgc cggtcagcga acgggcgtca gtgcctgcac
    82681 cgaagcactc atccacaatc atctgccccg gtttatgagc gtaaaccacg cgcccctggc
    82741 tgtcggtttc cagcaggcga ccaccgacgt tgagacaggc agtgccgctg atttttccgg
    82801 catcgaacag ggcgtagttc gcggtgccac cgccgatatc gatattcagt acccgacaca
    82861 gccgttgttc agaaagggtt tgcgccccgg ctccgtgacc ggcgatcacg gattcgaggt
    82921 gcggcccggc gctggcgacg acaaaatcgc ccagcgactg tttttccttc atcacttttt
    82981 ccatcagctg tgcgaccagg ctttcggtaa actggccttc cggcagctgg gcaatgatgg
    83041 tttcgcggat gcgctggctt tcggttttgt cggctggcac gctggcgggc acggacgccg
    83101 ttaccgggat cgattcatca cattcggtga tggtaaaacc cagtagatcc gccacttcac
    83161 gggcttccgg ggtaataatg ctggcgcgca gaaccacgga cattgcctgt tcgccgcgcg
    83221 cgtgtgcttc acgaatatca ttcgctgtga tgagtttttt cacctgcttc ctcctgtttg
    83281 gtgagagatg ccagataatc caccagttgc tgcacgcttt gcgggtcgtg gctgtttagc
    83341 tcgaaaatcg gctcttcgaa acccgtctca agcaataact ttcgcgttgc ggcgacgtca
    83401 gcatctggca tgtccgtttt actgatgacg gcgatttgcc gtttgctgac gccaatatcc
    83461 agcaacccgg caggcaaccg actttccggg tcatttgcgc catggacata gatcagcatg
    83521 tctacatcct gtagcgtggt aattaaggcg tgataccagc ggggatggct aaaatattca
    83581 cccggcgtat caatatcgcc cttatcatta aattccacgg ccagtgtttt tctggcgagg
    83641 gtataattcc cctgcaacgc attaaataac gttgtttttc ctgccccgac gctacccaca
    83701 aacgcaattc gtttcatgaa tgcctcgtat taacttttgg tcatttcaca aagcgtataa
    83761 tttaataagc gacctaaacc gctgacggtt tgtgataacg cttcttctac tgcgcccacc
    83821 gagccataaa tcaccaatgc accgctgaag cggtcgagaa aaccgatatg cacatcagcg
    83881 gctttcagcg ctaaatcacc ggcgatcatc gccgtttcgc cgggagttag cgtcataatg
    83941 ccaattgcgc ccgcatccgg aacgccgatt ttcttcgcca gttcctcacc gggatgcgcg
    84001 atgagatgcg ccagtgtgac ctgtttgccg ggcacaaatt cctgaatgat gcgttcttta
    84061 tccatgagtc gccgcctgtg aaaaactttc gtgtatcgtg acaatttttc gtcagagtaa
    84121 ataacaaaat tcggcaagcg cgtttaaaag gtgaggtaga tcaataagaa tggagagata
    84181 tttactgtta tgaataaact ttcatatgcg acgcatgaat ataaataaaa taatatcgag
    84241 atattttata tactgatttt tttattattc gcaaactcag taataagctt tgataatata
    84301 tttcaggaaa accgcttacc ctgaatattc agggtaagcg tgagagttaa gaatgaatta
    84361 cagcggttgg gtttgcgctt caaccacggc cagcgccacc atattgacga tacgacgcac
    84421 cgatgcgatc ggcgttaaca cgtgaaccgg tttcgccacg cccatcagca ccggaccgac
    84481 agtcacacct tccgagctgg aaacgcgcag taagttgtaa ctaatgcggg cagcttccat
    84541 gttcggcatc accagaatat tggcagaacc tttcaaaggg ctgtccggca tacggtcgtt
    84601 gcgaatcgct tccaccagcg ctgcatcgcc gtgcatttca ccatcaatca tcagatctgg
    84661 cgcacgatcc ctgaccagtt ccagcgcctg acgcattttg ctcgacgacg ggcagtcaga
    84721 agaaccaaag ttggagtgcg acaacagagc aacgcgcggc tcaataccaa aacgacggac
    84781 agtttctgcc gccatcaagg tgatctccgc cagctcttct gcgtctggtt cgtcattgac
    84841 gtaggtatcg gcaataaagg tgttaccact cggcagcagc agcgcattca ttgcacctgc
    84901 ggtgtgaacg ccatcgcgat aaccaaagac atttttcacc acactaaaat gttcatgata
    84961 atcgcccacc gtaccgcaga tcattgcatc ggcttcccca cgctgaacca tgatcgcgcc
    85021 gatcactgtc gggttactga tcagcgcacg ctgcgcctgt tcctgagtga caccgcgacg
    85081 cttcatgatc tggaagtatt cggtccagta ctctttaaag cgcggatcgg attcgttatt
    85141 gacgatttca aaatcaacgc ccgctttgat ctgcaagccc agtttctgaa tgcgcatttc
    85201 gatcacgttc ggacgaccga taaggatcgg tttcgccagt cccagcgtta ccagttcctg
    85261 agtcgcatgc agaacgcgcg cctcttcccc ttccggcaga actacgcgct tcggcgcttt
    85321 gcgagcctgg gagaaaatcg gcttcataaa caggttggtt ttgtaaacga actcagtcag
    85381 cttgtcgatg tagacgtcga aatcagcaat cggacgcgtc gccacgcccg actccatcgc
    85441 cgcttttgcg accgcaggag cgatcttaac gatcaagcgc ggatcaaacg gttttggaat
    85501 gatgtattcc ggaccaaagc tcagatcctg aacgccatac gctgaagcca ccacttcact
    85561 ctgttctgca tgggccagtt ctgcaatcgc gcgtaccgcc gccagtttca tctcttcgtt
    85621 gatggcggtt gcgccaacgt ccagcgcgcc acggaagatg aacgggaagc aaagaacgtt
    85681 gttcacctgg ttcgggtagt cggaacggcc ggtacagatg atggcatccg gacgcacttc
    85741 tttcgccagc ggcggcagaa tttccggttc cgggttcgcc agcgccagga tcattggtgc
    85801 ccgagccatt ttcttcacca tttcctgggt cagcactttc gggccggaac agcccaggaa
    85861 aatatccgca ccttcaatca catcatcgag ggtacgtttg ccgtcatcca ccaccgcata
    85921 tgcggctttg gtttccgcca tatttggctc acggccctga tagataacgc cttttgaatc
    85981 gcaaaccacg atgttatgtt tttgcagccc cagcgctacc agcaggttca tacaggcgat
    86041 tgctgcggca cccgcgccgg aaaccaccat ccgcacgtcg gagatgtttt tctccaccac
    86101 gcgcaagccg ttgaggatgg cggcagtgct gatgattgcc gtgccgtgct gatcgtcgtg
    86161 gaataccgga atattcatcc gctcgcgcag tttctgttca atatagaaac attctggcgc
    86221 tttaatgtct tcgaggttga tgccgccgaa ggtcggttcg agcgcggcga cgacttcaat
    86281 aaatttgtcc gggtcgagtt catcaacttc aatgtcaaat acatcaatcc cagcgaattt
    86341 cttaaataga acgcccttgc cttccatcac cggtttgcct gccagcgcgc caatgttgcc
    86401 taaccccagc accgccgtac cgttagagat caccgcaacc aggttaccgc gcgcggtata
    86461 tttgtaggct tttagcgggt ctttttcgat ttcaagacaa ggtgcggcaa cgcctggtga
    86521 gtaggccagc gccagatcgc gctgcgttgc cagaggcttg gttggagaaa cctggatttt
    86581 ccctggaact ggaaattcat ggaaatcaag tgcactttgt tttaactggt catccatttg
    86641 gttgttcctt tcacgtaacg ttcacaaata aagtgtgtgg gcaacagccc ctgcccacaa
    86701 cgtggcgcac attattaccc tgccggagtc tacagacttt gagcaagtcc aaactctcac
    86761 cattaatata atgttttggt aataatccta taacactgat gttacctgct taatccagca
    86821 ataccatgcc tgtctgctat gcttttttga tgcgtttagc gaaatttctc agaagtgtga
    86881 attaacgcac tcatctaaca ctttactttt caaggagtat ttcctatgaa cgagttagac
    86941 ggcatcaaac agttcaccac tgtcgtggca gacagctgcg atattgagtc cattcgccat
    87001 tatcatcccc aggatgccac cactaatcct tcgctgttac tcaaggctgc cggattatca
    87061 caatatgagc atttaataga cgatgctatc gcctggggta aaaaaaatgg caagacccag
    87121 gaacaacagg tggtcgcagc gtgtgacaaa ctggcggtca atttcggtgc tgaaattctc
    87181 aaaatcgtac ccggttgcgt gtcaacagaa gttgatgcac gcctctcttt tgataaagaa
    87241 aagagtattg agaaggcgcg ccatctggtg gacttgtatc agcaacaagg cgttgagaaa
    87301 tcacgcattt tgatcaaact ggcttcgacc tgggaaggaa ttcgcgcggc cgaagagctg
    87361 gaaaaagaag gtattaactg caacctgacg ctgctgtttt cttttaccca ggcgcgggcc
    87421 tgtgcggaag caggcgtttt tctgatttcg ccgtttgtag ggcgtattta tgactggtat
    87481 caggcacgca agccgatgga cccgtatgtg gtggaagaag atccgggcgt taaatcggtg
    87541 cgcaatatct acgactacta taagcaacac cactatgaaa ccattgtgat gggcgcgagc
    87601 ttccgtcgca ccgaacaaat cctcgcctta accggctgcg atcgactgac tatcgcaccg
    87661 aatttgctga aggagctgca ggaaaaagtt tctccagtgg tacgtaaatt aatcccgcct
    87721 tctcagacgt tcccacgccc agcacccatg agcgaagcgg agttccgttg ggagcacaat
    87781 caggatgcga tggcggtaga aaaactgtct gaaggcattc gtctgttcgc tgttgatcaa
    87841 cgcaaactgg aagatcttct tgccgccaaa ctataaacca gccacggagt gttatatgtc
    87901 ccgaaaagac cttgccaatg cgattcgcgc actcagtatg gatgctgtac aaaaagccaa
    87961 ttctggtcat cccggcgcgc cgatgggcat ggctgatatt gccgaagtgc tgtggaacga
    88021 ttttcttaaa cataatccta ccgacccaac ctggtatgat cgcgaccgct ttattctttc
    88081 caacggtcac gcgtcgatgc tgctctacag tttgctacat ctgaccggtt acgacctgcc
    88141 gctggaagaa ctgaagaact tccgtcagtt gcattcgaaa accccaggcc acccggagat
    88201 tggctatacg ccaggcgttg aaaccaccac cggcccgctt ggacaaggtt tggcgaacgc
    88261 cgtcgggctg gcgatagcag agcgtacact ggcggcgcag tttaaccagc cagaccatga
    88321 gatcgtcgat cacttcacct atgtgtttat gggcgacggc tgcctgatgg aaggtatttc
    88381 ccacgaagtc tgttcgctgg caggcacgct gggactaggc aagctgattg gtttttacga
    88441 tcacaacggt atttccatcg acggtgaaac agaaggctgg tttaccgacg atacggcaaa
    88501 acgttttgaa gcctatcact ggcatgtgat ccatgaaatc gacggtcacg atccgcaggc
    88561 ggtgaaggaa gcgatccttg aagcgcaaag cgtgaaagat aagccgtcgc tgattatctg
    88621 ccgtacggtg attggctttg gttcgccgaa taaagcaggt aaggaagagg cgcacggcgc
    88681 accactgggg gaagaagaag tggcgctggc acggcaaaaa ctgggctggc accatccgcc
    88741 atttgagatc cctaaagaga tttatcacgc ctgggatgcc cgtgaaaaag gcgaaaaagc
    88801 gcagcagagc tggaatgaga agtttgccgc ctataaaaag gctcatccgc aactggcaga
    88861 agagtttccc cgacggatga gcggtggttt accgaaggac tgggagaaaa cgactcagaa
    88921 atatatcaat gagttacagg caaatccggc gaaaatcgct acccgtaagg cttcgcaaaa
    88981 tacgcttaac gcttacgggc cgatgctgcc tgagttgctc ggcggttcgg cggatctggc
    89041 tcccagcaac ctgaccatct ggaaaggttc tgtttcgctg aaggaagatc cggcgggcaa
    89101 ctacattcac tacggggtgc gtgaatttgg catgaccgct atcgccaacg gcatcgcgca
    89161 ccacggcggc tttgtgccgt ataccgcgac gttcctgatg tttgttgaat acgcccgtaa
    89221 cgccgcgcgg atggcggcac tgatgaaagc gcggcagatt atggtttata cccacgactc
    89281 aattggcctg ggcgaagatg gtccgacgca ccaggctgtt gagcaactgg ccagcctgcg
    89341 cttaacgcca aatttcagca cctggcgacc gtgcgatcag atggaagcgg cggtgggctg
    89401 gaagctggcg gttgagcgcc acaacggacc gacggcactg atcctctcaa ggcagaatct
    89461 ggcccaggtg gaacgtacgc cggatcaggt taaagagatt gctcgtggcg gttatgtgct
    89521 gaaagacagc ggcggtaagc cagatattat tctgattgcc accggttcag agatggaaat
    89581 taccctgcaa gcggcagaga aattagcagg agaaggtcgc aatgtacgcg tagtttccct
    89641 gccctcgacc gatattttcg acgcccagga tgaggaatat cgggagtcgg tgttgccttc
    89701 taacgttgcg gctcgcgtgg cggtggaagc aggtattgcc gattactggt acaagtatgt
    89761 tggtctgaaa ggggcaattg tcgggatgac gggttacggg gaatctgctc cggcggataa
    89821 gctgttcccg ttctttggct ttaccgccga gaatattgtg gcaaaagcgc ataaggtgct
    89881 gggagtgaaa ggtgcctgat ggtgatcgcc ggatgctgta tgttcccctc accctaaccc
    89941 tctccccaga agggcgaggg gacggtccgt gcgcttttta gggccgcatc tgcgattttg
    90001 tttaacgggt gatccacaac gtgggcctgg cgtctggccc atgccagtta tcgcaggtag
    90061 gttctgcggc gtaacgcacc aggcgaaaac gctgaccgtc aaagcgccag cgcgcctgaa
    90121 tgccacaatc gcttaatccg cgccctttcg ctaaggtcac cagttcacgc gatttctcat
    90181 caaatgttgc gttcatcagt tccagttcat tcgtctcctg gccgttgttg aacggcaaac
    90241 gcaaccgaac cgggcgcgag gctagcggct ttttacgcga cacaatccat gccaaatcaa
    90301 tggtgttata ggcccctgcc tcacagctaa tcatcatcag tgctttatca tcggtcagcg
    90361 cagtgacatt cacctcacga cgtaacggat caagcgagca gcgcagacca ttcatccgcc
    90421 agtttccata atccagcaaa tcgttacgtt cttcgagtga gagtggcgtc ggcgttgggt
    90481 taaccaccgc gacctctttc agcgcaggcg cgggcggtac gctgagaggc ggttcgtccc
    90541 ctttcttgat ccacgcggtt tcactgccaa cgcgcttttg ctgagcatca ataaacaaca
    90601 acgccgcttt taagccgctc agagaaatgg tctgattgcc gtcgcgtaag gtgatcgcct
    90661 tcccttcctg aattatctgc aaaaacgcgg tgatggttgc cgtatcatcg gttactaata
    90721 accatggtga aatccgccac ttgtcaccac ttaatgccaa cggctcacca tctaatagca
    90781 gccgtggcgc tatctcccct tcagacgcat ccggcgactt caacccaccg cgctcaatac
    90841 gtaaaacggc atcggtatgc gccccagcgc tgcggctcag ggtcatcacc agtccattat
    90901 gatcgcccgt attgcgcgcc acacaaaaat tttgattatt acaggtgacc tgccagtcgg
    90961 aaaacgcccg ttgcgctggt gccgcccaca ccaaagacgt cggcaacaaa gcgaaaaaaa
    91021 agagaaggaa aatgcgatag cgcatggacg gtacgacccc agaagttcaa acaaacagca
    91081 caagtcgtat cttcgttgcg caggtagcgc ggctcaatcg gatttgtcgg attgagccaa
    91141 taataaacaa cgaaatatta ttgctatttt tcagtccatt aaatgtgaca tttgcaaata
    91201 gttaagcaat aacaccgtct taccgtcacg tatctcgccg gttttgatca tctccagcgc
    91261 ctggctgaac ggcagctcga gcacttcaat atcttcatct tcaacaccgc caccggcgtt
    91321 agcgcgctga ttgtcactgt attcggcgat aaaaaagtgg attagctcag tcacaccgcc
    91381 aggcgacata tacagttcaa ataatttgcg cacttcgccc acttcatagc ccgtctcttc
    91441 aatcgcttct ttacgaatgc acacttccgg ttcgtcgtta tccagcagcc cggcgcaggt
    91501 ttcaatcagc tgcccgcttt cattgccatt aacccaggta gcgacacgga actgacgaat
    91561 cagaaccacg ctctttttct tcgcgttgta caggaggatc gtcgcaccat taccgcgatc
    91621 gtaaacttca cgtttatggc ggataacttc gccatctttg cgcgtgagat cgtaagtaat
    91681 gttgtgcagg gtgaaatagt tatcggagag aattttgtct ttaatgaggg tgatttgttg
    91741 cgtcataccg actccacagc gcgaaatgaa caattatatt acgctgtgaa gtcgggtttg
    91801 tctgcgcagg ctatcagtga gatttgactg attttacccc cagccagtca ataatcccct
    91861 gtgccgcatg acgtccttct gccattgcgg taaccaccag atccgcacca cgcacggcgt
    91921 cgccaccggc gaagattttc ggattggtgg tctggtaacg gtactggctt tccacatccg
    91981 cgatgatgcg gccccatttg tctaccgtta caccgtgcga ctccagccac ggcatcccgt
    92041 gtggattgaa gccaaacgcc ataatcaccg catcggctgg catgacaaat tcactgccct
    92101 caatcggtac aggtcgccga cgcccctggg catccggctc gccaagacgc gtgcgcagga
    92161 aacgaattcc gcaaacgtga ccttgctcat tcagctcaag cgccaccggc tggacgttaa
    92221 attcgaagtt ggccccctct tcgcgggcgt tcttcacctc tttcttcgag cctggcatgt
    92281 tagcttcatc acgacgataa gcgcaggtga cgttactcgc gccgtggcgc agcgcagtac
    92341 gcacacagtc catcgcggtg tcgccgccgc ccagtaccac gacgttaagt ccggcggtat
    92401 tgataaacgg ctcttccggt agctcttcga gccccatcac ctgtttagtg ttggcaatga
    92461 ggaacggcag cgcgtcataa acgcccggcg catcttcatt gggtaaaccc gctttcatgg
    92521 aacggtaagt gcctacgcca acgaagaccg cgtcgtattg ttccaaaagc aaatccaaag
    92581 agacatcttt acccacttca caattgagtt cgaagtgaat ccccatcgcg ctgaagattt
    92641 cccgacggcg tgccagcagg gatttatcca gtttgaaaga aggaatgccg aaagtgagca
    92701 agccaccgat ttctggatgg cgatcgtaca ccgtcaccgc cacgccattg cgggtcagaa
    92761 catccgcaca ggccagccct gccggacctg ccccgataat cgccacccgc ttgtccactt
    92821 tggtgacatg acttaagtca ggacgccaac ctttcgccaa cgcctgatct gaaatatagc
    92881 gttcaatgtt gccgatagtt accgcgccgt gctcatcgcg aatagtacag gcaccttcac
    92941 acaaacggtc ttgcggacaa acgcgtccgg taatttccgg cagggtgttg gtctggtgag
    93001 aaagctcgac ggcggcgtcg atgtttccgg ctttcaccag ttcaatccac tgcggaatat
    93061 ggttatgcag cgggcaggtc cattcacaaa cgctgtgctc gccgaactta aggcagcgcg
    93121 aggcttcccg ttgtgcctgg tcggcgcgaa atggcagata aatttcatca aaaccggttt
    93181 tgcgcgcttc aatcgccagt ttatccggct cgccacgcgc gggcgttgcc tgcatttgtt
    93241 cgactttact cattaccggc atttcttgtg cagcggtact ggcatgccac ggttgatgct
    93301 cctgacgtgc ggtgcgcaag cggcgggatt tcgccatgcc ggagagtgcg acgtcagtga
    93361 ccagttgcag cgcgtccgcc gggcaattct cgacacaggc aggaccgttt tcgcgccctg
    93421 cacaaaggtc gcatttatgc gccgtggctt ttacttttcc tgccgcgacg ggtgtcagga
    93481 cgatttgcat cgtaccaaac ggacaggcca ccacgcagga tttacagcca atacactttt
    93541 gctgattgac ctgaatgctg tcatcaacgt ggctgattgc gccattaggg cagctacggg
    93601 cgcagggcgc atcttcacaa tggtgacagg tcactgcact acgttgctgc tgatgtttga
    93661 taaccgtaat tcggggatga aaatggtgtt ggctcaggac atgttgctca tcattgtgag
    93721 ccatgacaca ggcgatttca caagcatgac aacccagaca ttgctgactg ttggccataa
    93781 taaaacgatt cataacgacc ttcttttttg gttgtaaaaa ccttattctt tatatgagtg
    93841 ttgttattac ccgacttaca ggggaaccgg caatgttcat gtgcccagaa taagtaacta
    93901 tttcgctata aactgtggca gatcaaataa tcccatcact gactaaattg cgtttcagcg
    93961 aactggaaca ttaatgattt tttgtggaga agacgcgtgt gattgttaaa cgacccgtct
    94021 cggccagtct ggcccgggcc tttttttaca ttgtgctgct gtcgattctt tccacgggta
    94081 tcgctctgct aactctggcg agcagtttgc gcgacgctga ggctatcaat attgccggat
    94141 cgctgcgtat gcagagttac cgcctgggct acgacttgca aagtggcagt ccacaactca
    94201 atgcacatcg ccagttgttt cagcaggcac tgcattcacc ggtattaacc aacctcaacg
    94261 tctggtatgt gccagaagca gtaaaaactc gctatgcgca tctgaatgcc aactggctgg
    94321 agatgaataa tcggctcagc aagggcgatt tgccgtggta tcaggccaat attaataatt
    94381 atgttaatca gatagacctg ttcgtgctgg ctttacagca ctacgctgaa cgcaaaatgc
    94441 tgctggtggt ggcgatttcc ctggctggcg gtatcggtat tttcacgctg gtctttttta
    94501 ctttgcgccg catacgccat caggtggttg ccccgctgaa tcagctggtt accgccagtc
    94561 agcgtattga acacgggcag ttcgactcgc cgccgctgga taccagtctg ccgaatgagc
    94621 ttggtctgct tgcaaaaacc tttaaccaaa tgtcgagcga gctgcataaa ttgtaccttt
    94681 cgctggaggc gtcagtagaa gaaaagaccc gcgatctcca cgagaccaag cgtcgtctgg
    94741 aggtgttgta tcagtgcagt gttcccaggc gctgaacacc agccagattg atgtgcattg
    94801 tttccgccat attttgcaga ttgttcgcga caatgaagca gctgaatatc tggagctgaa
    94861 cgttggggat aactggcgga ttagcgaagg gaagccaaac cctgagctgc caatgcagat
    94921 tttaccggtg acaatgcaag agacggttta cggcgaactg cactggcaaa atagtcacgt
    94981 ttcatcatca gaaccgctgc ttaacagtgt ttcatcgatg ctgggacgcg gtttgtactt
    95041 taatcaggcg cagaagcatt ttcagcagtt attgttgatg gaagaacgtg cgaccatcgc
    95101 ccgcgaattg cacgactcgc tggctcaggt gctttcttac ttacgtatcc agttgacgtt
    95161 actgaagcgt tcgataccgg aagataacgc caccgcacaa agtatcatgg ccgatttttc
    95221 ccaggcgttg aatgatgctt atcggcagtt acgcgagctg ttgactactt tccgcctgac
    95281 gctgcagcag gcggatctcc cctccgcgtt gagggaaatg ctggatacgt tacaaaatca
    95341 aaccagcgct aaactgaccc tcgactgccg tctgccaacc ctggcactgg atgcgcaaat
    95401 gcaggtgcat ttgttgcaaa ttattcgcga agcggtgctg aatgcgatga agcacgccaa
    95461 cgccagcgaa atcgccgtca gttgcgtcac cgcgccggac ggcaatcaca cggtttatat
    95521 ccgtgataac gggattggta tcggtgaacc gaaagaaccc gaaggtcatt atggactgaa
    95581 tatcatgcgc gaacgcgcgg aacggctggg cgggacgctg actttttcac aaccttccgg
    95641 cggcggcacg ttagtgagta ttagctttcg ctccgcagag ggtgagggga aggggaacac
    95701 gccggctgag gtgtaaataa ttaccctcac accggccctc tccctttaaa ggcgagaggg
    95761 aaaaacgtgc ccatattgac ctgtaagcga gtgtgtcagc aatctcatga taatttacat
    95821 taactccttt ttttctccac gattggctcg taccttgccg ctacagtgaa gcaagtcaag
    95881 cctacaacaa tacgcagaaa cacgaggtcc tcttttaatg gcgaatttct ttattgatcg
    95941 ccccattttt gcctgggtgc tgacaatcct gttgtgtctg acaggtaccc tggcgatttt
    96001 ttcattgccc gttgaacaat accccgatct cgcgccaccg aatgtgcgag tgaccgctaa
    96061 ctatcccggc gcatcggccc agacgctgga aaacaccgtg acccaggtta tcgagcaaaa
    96121 tatgaccggc ctcgataatc tgatgtatat gtcatctcag agcagtggca ccggtcaggc
    96181 atctgtcact ttaagtttta aagcaggcac cgatccggac gaagccgtgc agcaagtaca
    96241 aaaccagctg caatcagcca tgcgaaagtt accgcaggcg gtgcaaaatc agggcgtgac
    96301 ggtgcgtaaa accggcgata ccaacattct gaccattgcc ttcgtctcta ccgatggctc
    96361 gatggataaa caggatattg ctgattatgt tgccagtaat attcaggacc cgttaagccg
    96421 cgtgaatggc gtcggggata tcgatgccta tggttcgcaa tattccatgc gtatctggct
    96481 ggacccggcg aaactcaaca gtttccagat gacggctaaa gatgtcactg atgccattga
    96541 gtcacagaac gcgcagattg cggttgggca acttggtggt acaccttccg tcgataagca
    96601 ggcgctcaac gccaccatta acgcccagtc actgctgcaa acaccagaac agttccgcga
    96661 tatcaccttg cgggtgaatc aggacggctc agaggtaagg ctgggcgatg tcgccaccgt
    96721 cgaaatgggg gcggagaaat acgattatct tagccgcttc aatggtaagc cagcctccgg
    96781 gttgggggta aaactggcct ccagcgcaaa cgaaatggcg acagcggagc tggtgctcaa
    96841 tcgtctcgac gagctggcgc agtatttccc gcacggactg gaatacaagg tggcgtatga
    96901 aaccacctcg tttgttaaag cctccattga agacgtggtg aaaacgctgc tggaagctat
    96961 cgctctggtt ttcctcgtta tgtatctgtt cctgcaaaac ttccgcgcca cgctgatacc
    97021 cactatcgcc gtgccggtgg tgttgatggg aaccttctcc gtactttacg ccttcggtta
    97081 cagcgtcaac accttaacca tgttcgcgat ggtgctggcg atcggtctgc tggtggatga
    97141 cgccatcgtg gtggtggaaa acgtcgaacg tattatgagt gaggaaggac tcactcctcg
    97201 cgaagccaca cgtaaatcga tggggcagat ccagggggca ctggtcggga ttgcgatggt
    97261 gctttcggcg gtatttgtac caatggcctt cttcggcggc accaccggtg ccatctatcg
    97321 ccagttctct attaccattg ttgcggcgat ggtgctgtca gtactggtag cgatgctcct
    97381 cactccggct ctgtgtgcca cgctacttaa gccactgaaa aaaggtgagc atcacgggca
    97441 aaaaggtttt tttgcctggt ttaaccagat gtttaaccgc aacgccgaac gctacgaaaa
    97501 aggggtggcg aaaattctcc accgtagcct gcgctggatt gtgatttatg tcctgctgct
    97561 tggcggcatg gtgttcctgt tcctgcgttt gccgacgtcg ttcttaccgc tggaagaccg
    97621 gggcatgttt actacctcgg tacaattgcc cagcggttca acccaacaac agaccctgaa
    97681 agtcgttgag caaatcgaga aatattactt cacccatgaa aaagacaaca tcatgtcggt
    97741 gtttgccacc gttggttctg gccctggggg taacgggcaa aacgtggcga gaatgtttat
    97801 ccgcctgaaa gactggagcg aacgcgacag taagaccggc acctcgtttg ccattatcga
    97861 gcgtgcaacg aaggcgttta accaaattaa agaagctcgc gttatcgcca gcagcccacc
    97921 agcaattagc ggtcttggta gttctgcagg ttttgatatg gagttgcagg accacgctgg
    97981 agcgggtcac gatgcgctga tggcagcacg taatcagttg ctggcgctgg cggcggaaaa
    98041 cccggagcta acccgtgtgc gccataacgg cctcgatgac agtccgcagt tgcagattga
    98101 tatcgaccag cgtaaagctc aggcgctggg cgttgatatc gacgatatta acgacacact
    98161 gcaaaccgcc tggggttcga gctatgtgaa tgactttatg gatcgcggtc gcgtgaagaa
    98221 agtctatgtg caggcagctg cgccgtatcg catgctgcca gatgacatca atctctggta
    98281 tgtccgaaat aaagatggcg gcatggtgcc cttctctgct ttcgcgacct cacgctggga
    98341 aacaggctcg ccgcgtctgg aacgctataa cggttattct gcggttgaga ttgttgggga
    98401 agccgcaccg ggggtcagta ccggtacggc gatggatatt atggaatcgt tagtgaagca
    98461 gctgccaaac ggctttggtc tggagtggac ggcgatgtcg tatcaggagc ggctttccgg
    98521 cgcgcaggct ccggcgctgt acgccatttc cttgctggtg gtattcctgt gtctggcggc
    98581 attgtatgaa agctggtcgg taccgttctc ggtaatgctg gtcgtgccgc tgggggtaat
    98641 cggcgcgctg ctggcaacct ggatgcgcgg gctggaaaac gacgtttact tccaggtggg
    98701 cctgttaacg gtcattggtt tatcggcgaa aaacgccatc ctgatcgtcg agtttgctaa
    98761 cgagatgaac caaaaaggcc atgacctgtt tgaagcgacg ctccacgcct gccgtcagcg
    98821 tttacgcccg attctgatga cctcgctggc atttatcttc ggcgtattgc caatggcaac
    98881 cagcacgggt gccggttccg gtggtcagca tgcggtgggt actggcgtaa tgggcgggat
    98941 gatttcagct actattctgg ctatttactt cgtgccgctg ttctttgtgc tggtgcgccg
    99001 ccgcttcccg ctgaagccgc gcccggaata agcaataaaa aaggcgacat gccaatgtgt
    99061 cgcctttttc aactttccga ttaagaacct gctcagcggg ttcttgctgt ttatactttg
    99121 tctcaggaat tacttacgaa gcataacttc gataaagtct ttccagttcc ccagttcacg
    99181 ttcaatcata acaacctctc ttataattat gggtattcta cggaaacaat ataccgtggt
    99241 gaagctaatt tactcgattg ctgcgatgac tacctccggg ggacaaacct tatgtaaata
    99301 ctatggtcct gcggtgataa tttgtatgtg atacacagca acatttcgag atattcatac
    99361 ggcatctaat actgatttaa ttctggttaa aatacagaca gataacaaga tgaatattct
    99421 taatgtttac gttaaaaatg tttaatatta tttaatagtt gttaatttga atacttcgat
    99481 aatgttatat tttctgataa tcatttgcag gcaaaatgtt ttcaccctta aatgagtatt
    99541 tattctcata aatcgaaaaa ggattcatta tggttacact ttacggcatc aaaaattgtg
    99601 acaccattaa aaaggctcgc cgttggctgg aagccaataa catcgactat cgttttcatg
    99661 attaccgcgt cgatgggctg gacagcgaat tattgaacgg ttttatcaac gaattaggct
    99721 gggaagcgtt actcaacacc cgtggtacaa cctggcgtaa actggacgaa actacccgca
    99781 ataaaatcac cgatgcggcc tctgcggcgg cattaatgac tgaaatgcct gcaattatca
    99841 aacgtccatt gctctgcgcg cctggtaagc ctatgctgct gggtttcagt gattccagtt
    99901 atcagcaatt tttccatgag gtgtagtcta tgtcgtgccc ggttattgag ctgacacaac
    99961 agcttattcg ccgcccttcc ctgagtcctg atgacgcagg atgtcaggct ttgttgattg
   100021 aacgtttgca ggcgatcggt tttaccgttg aacgcatgga ttttgccgat acgcagaatt
   100081 tttgggcatg gcgtgggcaa ggtgaaacgt tagcctttgc cgggcatacc gacgtggtgc
   100141 cgcctggcga cgccgatcgt tggatcaatc cgccgtttga acccaccatt cgtgacggca
   100201 tgttattcgg gcgcggtgcg gcagatatga aaggctcgct ggcggcgatg gtggtagcag
   100261 cagaacgttt tgtcgcacaa catcccaacc atacggggcg actggcattt ctgatcactt
   100321 ctgatgaaga agccagtgcc cacaacggta cggtaaaagt cgtcgaggcg ttaatggcac
   100381 gtaatgagcg tctcgattac tgcctggtcg gcgaaccgtc gagtatcgaa gtggtaggtg
   100441 atgtggtgaa aaatggtcgt cgcggatcgt taacctgcaa cctcaccatt catggcgttc
   100501 aggggcatgt tgcctaccca catctggctg acaatccggt acatcgcgca gcacctttcc
   100561 ttaatgaatt agtggctatt gagtgggatc agggcaatga attcttcccg gcgaccagta
   100621 tgcagattgc caatattcag gcgggaacgg gcagtaacaa tgttattccg ggtgaactgt
   100681 ttgtgcagtt taacttccgc ttcagcaccg aactgactga tgagatgatc aaagcgcagg
   100741 tacttgccct gcttgaaaaa catcaactgc gctatacggt ggattggtgg ctttccgggc
   100801 agccattttt gaccgcgcgc ggtaaactgg tggatgcggt cgttaacgcg gttgagcact
   100861 ataatgaaat taaaccgcag ctactgacca caggcggaac gtccgacggg cgctttattg
   100921 ctcgcatggg ggcgcaggtg gtggaactcg ggccggtcaa tgccactatt cataaaatta
   100981 atgaatgtgt gaacgctgcc gacctgcagc tacttgcccg tatgtatcaa cgtatcatgg
   101041 aacagctcgt cgcgtgatga gtggttctgc aagaggaaat aagcatggac tggctggcta
   101101 aatattggtg gattctggtg attgtctttt tggtaggcgt cctgctgaac gtgattaaag
   101161 atctcaagcg cgtcgaccat aagaaatttc tcgccaacaa gccggagctt cccccgcatc
   101221 gtgatttcaa cgataagtgg gacgatgacg acgactggcc gaaaaaggat caaccgaaga
   101281 agtaagctga gtgctcaacc gtaggccgga taaggcgtta acgccgcatc cggcaatggt
   101341 gaacgatccc tgatgcgacg ctcacgcgtc ttatcaggcc aggtaatctg tcttttacat
   101401 catttcaatc acatcatcat cgccaggctt accgccactt agcgcttggt cgaaataatg
   101461 tttcggaatg gtataacgca agtgatccag ggcaaattgc atactgcgat tgtcgatcgc
   101521 atggcctaaa tcctccacga tatccagcgt cacgtcacca ccggcactga ttaacgcttc
   101581 ctgcgccgca actgcatgcg ccagatcgat aaccggatct tcaccaccat gaatcaagtg
   101641 aattgtcgtg gctgtagacg cggtctccgg caggctcgca tagcgtccgt taaaggcaat
   101701 gacgcgcgaa gcaaggcccg gttcggcttt aatgctctcc agcgccataa tcgcgccttg
   101761 tgagaaacca attagcgccg tggcgttagc ccctactccg ctctgtttct gccagtagcg
   101821 taccgtttca ataaacgtcg gcatgatggc atccacacgc gcctggcgat tatcttccgt
   101881 aataccctgc actgaaaacc actgacgccc cgccggatta ccgctcggct ccgcgccacc
   101941 gacactcacc accagcgcat caggaaacag cggtgcaaac cagctgccta tttcccccat
   102001 tgccaccggg ttatccccga caccgtggaa aagcagtaac aactgttgtg caggtttatc
   102061 cgggctttga acaacaaaat ggtcatgttt catggcgaac tccttaactg atgtcattaa
   102121 ttttacgccg ctgactcaca atgaccatgg gagaaaattg aaggagtcag tgaaaaaatt
   102181 gccattgcgt tatgcgatcg cgcagacgct cagtgcgaac ctcatttagt gcgaacagcg
   102241 cctgcgcggc ctcttcccgc tggcgaacca gtaacatctt gcggcctgaa agtttaagtg
   102301 tggtacataa ctgcgcgtca ctggcgtttt tctgtaaacg cccacgcagc gccggtaatg
   102361 ccagttcact ggtttgtagc agacgcaata agcaacctaa cgatgttaat agcggacgat
   102421 gagcgaaagc aaaaccggcc agttcaagcc agtcgtcgtc agaaaggatg gcatcgttta
   102481 gtggatcaac aggaagcatt tcgccattcc actgcgcgag agcttgcgca tcgcgacgta
   102541 aacggtaatg ctcacgttca gccagctgtt tacccgcatc actcatcggt aacagcgcca
   102601 tcgccgtata gcaaccgctg ctggcttccc gatgattacc catccgcacc agcacaaaac
   102661 cgcagcgttg ccagaaacgc cataactccc cagtgtaacc aaaactcacc gaaagatagt
   102721 cgaggtcatg cgtatattgc aaagctccag caataagttg tcgccctgtg ccttcccgct
   102781 gacgagccgg atgaactgct atccggctaa cccgccttcc gcgcaatgtc gccgccagtg
   102841 gattgctgcc gtgcgccgcc agcgactggg ccaccagatt accccgcggg cgacgaaaac
   102901 ctgcccatac cgcctgactg agttcttgag ataatccgcc ctcatccacc agccacaacg
   102961 ccccggcaat ctcgttttcg ccagccgcct gtaaaaaatg ttgccctggt gcatccatca
   103021 tccggcgtaa atccagcggc gaggtccggt agtgcgcacc agacaataac tgataaacct
   103081 ttaacggcgt ttctggctcg cttcgccata acgtttgttc aaatgcggaa atgacgatat
   103141 tgccttgtgg tgtatgggtg aagttttcat cgttaaaaac cagtgcctca ctaaccattt
   103201 tttccagcgg gcatccctgt gcccagcgga tcggctgttg cagttcaaaa cggtgtaaat
   103261 gcggaaagcg agcgcaaaat ttcagcaaaa aaccacgtcc ggtaccttcg tagccctgca
   103321 ccgtagtggt taacaacgtt cgaggaaaac gcgataccag ttgatgcaac aacggcgcag
   103381 gtatggctgc ggcttcgtcg accaccagcc agtcggcttg ctcatcgctg gctaacaagg
   103441 catccggtgc aataaagcga aacttctcgc ccgcaaattg tgccagtaca tccgttgccg
   103501 cttttgcggg cgcggtgaca atcgcactgc ccgcaatacg agaaatgagt tgccctgcca
   103561 acgccgactt accgcgccca cgcgcagccg ttaccgctgc cacgcccggc ggcatggtca
   103621 gtagctgctg taagagttgc tgttgttctg gttgtggtgc gccggtcgcg gggtgccagt
   103681 cagtacgggg agtaaaatgc gccaacgaga acggctggtt ttgccgccag aggatagcgt
   103741 cgttatccgc cgtaagtacg cgtttgagat gctggacaaa atgcggcgtc gcaatagggt
   103801 cagggcaatc actccagcgc agcgagtcgg catcaggttg gttttcccac tcttcccata
   103861 cagggagtaa caaaaccagc cagcttcccg ctttcaacgt tccgctaagt gctgcaaagg
   103921 cagcggcatc aaagccgtgg cgggcgtcga ataccgcatg ccggaactcg cgcccaagta
   103981 aagtttgtag cgccgagggt gaacagtggt tttcagcatc tagctgcggc gaaatccaca
   104041 gccagtcgcc aggtaaggca tcacgcaact taagagcatg atcaaaacac caaccctctt
   104101 ccccgctcaa caccagcaag cggcagatcc cttcacgttt catttgcgct gttaatgtgt
   104161 aaagcgcagt cagttcagcc atccctgccc cggaaattaa atgcttttac caaaagtatt
   104221 gcattgtgcc ggatcgccgc tgtcgaaacc acgtttaaac cagctgtagc gttgctgaga
   104281 agtgccatgt gtgaaactgt ctggtactac tcgcccctga ctttgctgtt gtaaacggtc
   104341 atcgccgatg gcctgcgccg cattcagcgc ctcttccaga tcgccagttt ccagaacgcc
   104401 ttgctgctgc atactatgcc cccagacacc ggcaaaacag tcggcctgga gttccatacg
   104461 cacagataag cggtttactt ccgcctgcgt cgcgttttgt tgcagttgac gaactttcgg
   104521 ctcgatgcct aacagtttct gcacatgatg accgacttca tgggcgataa cgtacccctg
   104581 ggcaaaatcg ccatccgcgc caagtttgtc tttcatgtca tcatagaagg agagatcgat
   104641 ataaactgtg ccatccgccg ggcaatagaa cggccccata atggactggc ccgccccgca
   104701 gccggtgcgc gtcattccac ggtacatgac cagcttcggt tgctgatagg tcttacccat
   104761 cttttcgaac tgttgtcccc aggtgtcttc cgtggttgcc tgaatcaccg aggtgaattt
   104821 tgcggcttcg tcttcattag ggctaattga ccgcgttgat tgttgttgag aaaccggctg
   104881 cccggtcatc aacccggtta aatcaacgcc atagtagcct gcaaccagca cgactatcag
   104941 taaaatcagc ccgcctttac cgctcggcag acggaaaccg ggaccgccca ttgacggacc
   105001 gccagagctg ttgcgcctgt cttcaacatt gtcactttca cgtcgccctt gccaacgcat
   105061 agatacctca acaatatatt cattatagaa atgatcgtag gtggttcaga ggaagattac
   105121 cacaggaaag cgggatgaaa tggcggagga atgagcgttc aatgcatata ttccaagagc
   105181 ggttgtcttg ccgtgccagc tgcacggcaa gatgatgaac agaaaaatca gtccagctgt
   105241 acacccaggc ggcgggcgac ggcttcatag gcttcgatca ggccaccgag gctctggcgg
   105301 aaacggtctt tgtccatttt ctccagtgtt tctttgtccc acaggcggct gccgtccggg
   105361 gagaactcat cgcccagtac cacttcacct ttgtacagac caaactccag cttgaagtcg
   105421 accagaatca gaccagcatc atcgaacagt tttttcagca cgtcgttcgc tttgtaggtc
   105481 agctctttca tacgcgccag gttctctttg ctcacccagc caaaggtttc gcagtaagat
   105541 tcgttgacca tcggatcgtg catggcatcg tttttcagga acagatcgaa cagcggcggg
   105601 ttcagctcaa taccttcttc gattccaaga cgtttcacta gagagccagc agcacggtta
   105661 cgcacgacac actcaaccgg caccatatcc agctttttca ccagacattc ggtatcggag
   105721 agcagacgct ccatttgagt cgggataccc gcttcagcca gtttgctcat aatgaagtag
   105781 ttgaacttgt tgttcaccat acctttgcga tcaaactgct caatgcgcgc gccatcccct
   105841 gctgacgtat cattgcggaa ttcgagcacc aacaggtccg ggttttccgt gctgtatacg
   105901 gttttcgctt taccacgata caactcagct tgcttttgca tctttatcac tcctgggtgt
   105961 gaattaacgt tttaaaatct tttgctgtct ggtgtgccgg atgttttgtc ggatgcggcg
   106021 tgaacgcctt atccgaacta cgtccgacaa acaaatttcg tgcgaattac cgctaaaatc
   106081 gcgttttcct gccgacgcac acgtttgcgt atcatatcag aaaaaagggc cggatgattc
   106141 cagccctgta tttttacttg ctaaacgcag cctggaagac agctaccagc gcgtcgttct
   106201 gactctgagt cagagtatga cctttcggat cgatgaactg taggctgctg cggttatcta
   106261 aatcgccaac ctgcagttta tagtcaccgg atgccaggcc tggatcgctc gcgcccagtt
   106321 cctgccagtc gctgtcagac agcggcttat aagttacggc catgttgccc tgcgaacggg
   106381 tgctgtcggt cactttcatg cccacttttt ccagcgccgc tggcagacgt tgccaaacca
   106441 cattgaacgg cccgcgtacg accagcattg gtaaaccggt gtcatcagct gcactttgta
   106501 cgtccatagt ggtggaggca cgattttgcg cagcgttcgc ggcgtcagtg gcagatttat
   106561 ccagaccggc ggaaataacg ttcatcatct ccgtgctgta acgctgcatg gaagccgcgt
   106621 ctgcaaccgg tttgcccgcc tgttccaggt tcagcagttt aaccgtaacc gcctgctgat
   106681 aaccctgcgg cttaacagag atttgataac gaccacgata ctgctcgtct tcgtccagac
   106741 ggttccattg tacccaatcg gtggtcagtg tctgaccagc atcatcacgt tgggtgatgg
   106801 tgtagttttt cgcctgcagc acgctaacca cctgcggcca cagagtattg ccacgaccat
   106861 tttccaccag caatgaagcg gtatcgcccg tgaactgggt acgcgcgcca gaaaccagtg
   106921 ccaacggctg ggctggtgga cgaatgtcca gcgccttacc gacagcacca ctaccgttgg
   106981 tcaccgggat tgcataatca ccggaggtca ccggcaaaat cattccagcc ggggcatgaa
   107041 gctccgcaag cggtgccgct tccaggtagg cttcatcacc actgacctga cgcttatagc
   107101 gtgagtcaga actacaggca gcgagtaata aaacaagcga aacacccgca acctttgcca
   107161 ggcgcgactt ttgaacagag taagccatca aatctcccta aactttacag caaaccggca
   107221 tgcttaagcg ccgctctgac cgtctcacga ccactgtcgg tgattggtgt cattggcagg
   107281 cgcagcgtat cggtcgccac aagacccagt tccttacatg cccatttcac cgggattgga
   107341 ttgggttcga caaatagttt gttgtgtaat ggcatcagac gctgattaat aacgcgtgcc
   107401 tcggcaaaat gcccttctgc tgccagtttg cacatctggg ccatatcacg cgctgcgacg
   107461 ttagccgtaa cggaaataac tccatgaccg cccaattgca tgaagtccag cgcgctcgca
   107521 tcatcgccgc tcagcagaac aaaatcatct gaaaccagct ctttgatctg gtttacacgc
   107581 gttaagttcc ctgttgcctc tttgattccg ataatatttt ttactttcgc cagacggccc
   107641 accgtttccg ggagcagatc gcagccagta cgggacggca cattatacag aatttgcggc
   107701 aggtcagtat gctcagcgat ggctttgaaa tgctgataca aaccttcttg cgacggacga
   107761 ttgtagtaag gggttaccgt caggcagccg acgataccac tgtcattgaa gcgctgcgtc
   107821 aggctaatgg cttccgcagt agcgttagcg ccggtcccgg caattaccgg aatgcgccca
   107881 tcagccagat ccagcgtcat catcaccaca tcagcatgtt cgtcatgatt taaggtagcg
   107941 gactcgccag tggtgccaac agaaacgatc gccgaagtac cgctggcgac atgataatca
   108001 atcagttttt tcaagctagc ccgacagaca ttaccttttt catccatcgg agtaacaatc
   108061 gcgacaatac ttcccgtgaa catgggccat cctctgtgca aacaagtgtc tcaatggtac
   108121 gtttggtatg gcattaaaag caagcagaca gaaccgttct gattgttgta tgcatgattt
   108181 ttttatgctt tccttaagaa caactcaccc cttaaaggaa taaccagttt gacactgtca
   108241 tcgcaacatt atctggtgat cactgcgttg ggtgccgatc gccctggaat tgtgaacacc
   108301 atcacccgtc atgtcagtag ttgcggctgt aatattgaag acagtcgcct ggcgatgctg
   108361 ggagaagagt tcacgtttat catgctgctt tccggttcat ggaatgccat cactctgatt
   108421 gaatcaacgt taccgttgaa aggtgccgaa ctggatcttt taatcgtgat gaagcgcacg
   108481 acggcgcgtc cgcgtccgcc aatgccagca tctgtctggg ttcaggtcga tgttgcagac
   108541 tccccgcatt taattgaacg cttcacagca cttttcgacg cgcatcatat gaacattgcg
   108601 gagctggtgt cgcgcacaca acctgctgaa aatgaacggg ctgcgcagtt gcatattcag
   108661 ataaccgccc acagccccgc atctgcggac gcagcaaata ttgagcaagc gttcaaagcc
   108721 ctatgtacag aactcaatgc acaaggcagt attaacgtcg tcaattattc ccaacatgat
   108781 gaacaggatg gagttaagta atgaatccac tgaaagccgg tgatatcgca ccgaaattta
   108841 gcttgccgga tcaagacgga gaacaagtta atttgaccga cttccaggga cagcgtgttc
   108901 tggtttattt ctacccgaaa gccatgaccc ccggctgtac cgtacaggcc tgcggcttac
   108961 gcgataacat ggatgagttg aaaaaagcgg gcgttgatgt gctgggtatc agcaccgata
   109021 aacccgaaaa actctcccgt tttgcggaaa aagagctgct taactttacg ctcctgtctg
   109081 atgaggacca ccaggtgtgc gaacaattcg gcgtctgggg tgaaaagtcc ttcatgggca
   109141 aaacctacga tggtattcat cgcatcagct tcctgattga cgctgatggc aaaatcgaac
   109201 atgtctttga cgatttcaaa accagcaatc accacgacgt tgtgctgaac tggctgaaag
   109261 aacacgcctg attactttgc tccattccgt gctggctgcg cttgcggcca gcatacctca
   109321 cttctcgtga tcaagatcac attctcgctt tcccctgcga cacgggtgtc gaatccattt
   109381 tttgctgaac gttaatgacc atcatttttg taccgttcag aatccagtta atacataact
   109441 tattgaatag attgagttaa tcagaatggc atcctttata caatatgaaa tgcaatgttt
   109501 catatcattt tcaaggagcc gacatgaacc gctttgtggt ggccgaacca ctgtggtgta
   109561 caggatgtaa tacctgtctc gctgcctgtt cggacgtgca taaaacgcaa ggtttacagc
   109621 aacacccgcg cctggccctg gcgaagacgt caacaatcac tgcccctgtc gtgtgtcatc
   109681 actgtgagga agccccttgc ctgcaggtct gcccggtcaa tgccatctct cagagggatg
   109741 atgcgatcca actcaacgaa agcctctgta ttggctgcaa gctttgcgcc gtggtctgcc
   109801 catttggcgc aatcagcgct tcaggaagcc gtccggtgaa tgcccatgcg caatatgttt
   109861 ttcaggctga aggctcactc aaagacggcg aagaaaacgt gccgccccaa catgcgttgc
   109921 tgcgctggga accgggtgtc cagaccgtcg cggtgaaatg cgacctgtgt gatttcttgc
   109981 cagaaggtcc ggcctgcgtt cgcgcttgcc cgaatcaggc gttacggctg atcaccgacg
   110041 atagcctgca acgtcagatg aaagaaaaac agcgccttgc cgcaagctgg tttgccaatg
   110101 gcggggagtc tcccctttcc ctcactcagg agcaacacta atggatgccc tgcaattatt
   110161 aacctggtcg ctgatcctct atctgtttgc cagtctggct tcgctgtttt tactcggtct
   110221 ggacagactg gctattaagc tttccggcat cacatcgctg gtgggcggcg tgattggcat
   110281 catcagcgga attacgcaat tacatgctgg tgtaacttta gtcgcccatt ttgccacccc
   110341 ttttgacttt gccgatttaa ccctgcgaat ggatagcctc tcggcattta tggtgctagt
   110401 tatctccttg ctggtggtgg tttgttcgct ctattcattg acttatatgc gcgaatacga
   110461 gggcaaaggc gcggcggcga tgggcttctt tatgaatctt ttcatcgcat cgatggttgc
   110521 cctgctggtg atggacaacg ctttttggtt catcgtgctg tttgaaatga tgtcgctgtc
   110581 ttcctggttt ctggtcattg ccaggcagga taaaacgtcg atcaacgctg gcatgctcta
   110641 cttttttatc gcccacgccg gatcggtgct gattatgatc gccttcttgc tgatggggcg
   110701 cgaaagcggc agcctcgatt ttgccagttt cgcacgcttt cactttctcc ggggctggcg
   110761 tcggcggtgt ttctgctggc ctttttcggt tttggcgcga aagccgggat gatgccgttg
   110821 cacagctggt tgccgcgcgc tcaccctgcc gcaccatcgc acgcttcggc gttgatgtct
   110881 ggcgtaatgg tcaaaatcgg tattttcggc atcctgaaag tagcgatgga tctgctggcg
   110941 caaacgggtt tgcctctgtg gtggggcatt ctggtgatgg cgatcggcgc aatctccgcg
   111001 ctcctgggcg tgctatatgc gctggcggaa caggatatca aacggctgct ggcctggagt
   111061 accgtcgaaa acgtcggcat tattttgctg gcagtcggtg tggcgatgga cggtctgtca
   111121 ctgcacgacc cgctgctcac cgtggttgga ctgctcggcg cactgtttca tctgctcaac
   111181 catgcgctgt tcaaagggct gctatttctc ggcgcggacg cgattatttc gcgtttgcat
   111241 acccacgaca tggaaaaaat gggggcactg gcgaaacgga tgccgtggac agccgcagca
   111301 tgcctgattg gttgcctcgc gatatcagcc attcctccgc tgaatggttt tatcagcgaa
   111361 tggtacacct ggcagtcgct gttctcacta agtcgtgtgg aagccgtagc gctacaactt
   111421 gcgggtccta ttgctatggt aatgctggca gtcactggtg ggctggcagt aatgtgcttc
   111481 gtcaaaatgt acggtattac tttctgtggt gcgccgcgca gtacacacgc tgaagaggca
   111541 caggaagtgc caaatacgat gatcgtcgcc atgctactgc tcgcggcact ctgcgtatta
   111601 attgcgctta gtgccagttg gctggcaccg aagataatgc atattgccca tgcgtttacc
   111661 aatacccctc ccgtcactgt cgccagcgga atagcacttg tacccggcac gtttcataca
   111721 cgggtcactc cttcattact gttgctgtta ctactggcga tgcctttgct gccaggcctt
   111781 tactggctgt ggtgtcgttc gcgccgcgca gcgtttcgtc gcacaggaga tgcctgggca
   111841 tgcggctacg gctgggaaaa cgcgatggct ccgtcaggca atggcgtgat gcagccgctg
   111901 cgtgtggtct tctctgcgtt atttcgtcta cgacaacagc tcgaccctac gctgaggcta
   111961 aataaaggtc ttgcgcacgt caccgccagg gctcagagca cagaaccctt ctgggatgag
   112021 cgggtgatcc gccccatcgt gagcgccacc caacggctgg tcaaagaaat acagcatctg
   112081 caaagcggcg actttcgtct ctattgcctg tatgtggtcg ccgcactggt tgtgctgcta
   112141 atcgctattg ccgtctaagg aaatcaccat gagacaaact ctttgcgacg gatatctggt
   112201 catttttgcg ttagcacagg ccgtgattct gctgatgcta accccacttt ttacgggtat
   112261 ttcccggcag atacgcgcgc gtatgcactc ccgccgcggg ccggggatct ggcaggatta
   112321 tcgcgatatc cacaaactgt ttaaacgcca ggaagttgcg ccgacatctt caggtctgat
   112381 gttccgcctg atgccgtggg tattaatcag cagcatgctg gtgctggcga tggccttacc
   112441 actatttatt accgtttccc cttttgcggg cggcggcgat ctgatcaccc ttatctatct
   112501 tcttgccctg tttcgttttt tctttgctct ttccgggctg gataccggaa gtccgtttgc
   112561 gggagtcggt gccagtcgcg agttgacgct cggcattctg gtcgaaccaa tgcttattct
   112621 ctcactgctg gtattggcgc tgatagcagg ttccacgcat atcgagatga tcagcaatac
   112681 gctggctatg ggctggaact cgccgctaac caccgtactg gcgttactgg cctgtggttt
   112741 tgcctgcttc attgagatgg gaaaaattcc ctttgatgtt gctgaagcag aacaggaatt
   112801 acaggaaggc ccgctgaccg aatattccgg tgccgggctg gcgctagcga aatgggggct
   112861 ggggctgaaa caggtcgtga tggcagcact gtttgtggcc ctgtttctgc cctttgggcg
   112921 cgcgcaagaa ctttctctcg cctgcctgct gacttcactt gtcgttacgc tgctcaaggt
   112981 tttgctgatt tttgtactgg cctcaatcgc agaaaacacg ctggcacgcg ggcgtttttt
   113041 actcattcac catgtgacct ggcttggctt cagccttgct gcgcttgcat gggtcttttg
   113101 gttaaccggt ctgtaaggag cactgacgga atatggaaaa tcttgctctg acgacgttaa
   113161 tgctgccttt tatcggcgca ctggtcgttt cgttttcgcc acaacgtcgg gccgccgaat
   113221 ggggggtttt gttcgccgcg ctgaccacgc tgtgcatgtt gtcgctgatc tccgcgtttt
   113281 atcaggccga taaagttgcc gtcacgttga cgttggtcaa cgtgggggat gtggcattat
   113341 ttggcctggt cattgatcgc gtgagtacgc tgattctgtt tgtggtggtg tttctcggtt
   113401 tgctggtcac gatctactcc acgggttatc tgacggataa aaatcgcgaa cacccgcata
   113461 acggcacgaa tcgttattac gcatttttgc tggtgtttat cggcgcgatg gcgggactgg
   113521 tactctcctc gacgctgctc ggtcagttgt tgttttttga aattacgggc ggctgctcct
   113581 gggcgttgat cagttattac cagagcgata aagcgcagcg ttcagcacta aaagcgttgc
   113641 ttatcaccca tatcggttcg ttggggttgt atcttgccgc cgccacgctg tttttgcaga
   113701 ccggaacgtt tgtgcttagc gcgatgagcg agttacatgg cgacgcacgt tatctggttt
   113761 atggcggcat cctgtttgcc gcgtggggga aatcggccca gctaccgatg caagcgtggc
   113821 taccggacgc aatggaagcg ccaacaccga tcagcgccta tctccacgcc gcatcgatgg
   113881 tgaaagtggg cgtttacatt tttgcccgcg ccattatcga cggcggcaat atcccgcatg
   113941 tgattggcgg cgttggcatg gtcatgacac tggtcaccat tctttatggc tttctgatgt
   114001 atttgccaca gcaggatatg aagcggttgc tggcctggtc gaccatcact caacttggct
   114061 ggatgttctt cggcttgtcg ctctccatct tcggctcgcg gctggcgctg gagggtagca
   114121 tcgcctacat cgtcaaccac gcgttcgcta aaagcctgtt tttccttgta gcaggtgcgc
   114181 tgagttacag ctgcggcacg cgcttgttgc cgcgtctgcg tggcgtattg cacaccctgc
   114241 cgttgccagg cgtgggtttc tgcgtggcag cgctggcaat taccggtgtg ccgccgttca
   114301 acggcttctt cagtaaattc ccgctgtttg ccgccggttt tgcgttgtca gtggagtact
   114361 ggatcctgct gcccgccatg attctgctga tgattgaatc ggtcgccagt ttcgcctggt
   114421 ttattcgctg gtttggtcgc gtcgtgccgg gcaaaccgag cgaggccgtc gccgatgccg
   114481 caccgctgcc agggtcaatg cgcctggtgt tgattgtact gattgtgatg tcgctgattt
   114541 ccagcgtaat cgccgcgacc tggttgcagt aaggagatga tgaatgaccg gttctatgat
   114601 cgtaaataat ctggcgggac tgatgatgct gacatcgctg tttgtgatta gcgtcaaaag
   114661 ctatcgcctg tcatgcggat tttacgcctg ccagtcgctg gtgctggtgt ctattttcgc
   114721 cactctctcg tgcctgttcg ccacagagca actgctgatc tggtccgcca gcgcctttat
   114781 caccaaagtg ctgctggtac cgttaatcat gacttacgct gcacgaaata ttccccagaa
   114841 catcccggaa aaagcgttat tcggtccggc aatgatggca ctgctcgcgg cgttaattgt
   114901 cctgctttgc gcatttgtcg ttcagcccgt gaagctaccg atggctaccg ggctgaaacc
   114961 ggcgctggcg gtagcgttag gtcattttct gcttggcctg ctgtgcattg tcagccaacg
   115021 caatatcctg cggcaaattt ttggttactg cctgatggaa aacggctccc atctggtgct
   115081 ggcgcttctt gcctggcgag caccggaact ggtggaaata ggtatcgcta ccgacgccat
   115141 cttcgccgtc attgtgatgg tgttactggc aagaaaaata tggcgtaccc acggcacgct
   115201 ggacgtgaac aacttgaccg cgctgaaggg ataatgagat gagttattct gtgatgttcg
   115261 ctttactcct gctcacgccg ctgctttttt cgctgctctg ttttgcctgc cggaaacggg
   115321 ggctctctgc gacttgtacg gtgactgtat tacatagctt agggatcaca ctgctgctga
   115381 ttctagcact ctgggtggtc caaactgccg ctgatgcagg agaaatattc gctgcgggac
   115441 tgtggcttca tattgatggt ctgggcggtt tgttcctcgc cattcttggt gtgattggct
   115501 ttctcaccgg tgtttactcg attggctaca tgcgtcatga agtggcacac ggcgagcttt
   115561 cacccgttac actgtgcgat tactacggtt tcttccatct gtttttgttc accatgctgc
   115621 tggttgttac cagcaataac ctgattgtga tgtgggcggc gatcgaagcc accaccttaa
   115681 gctcggcgtt tctggtaggc atttacggtc agcgttcatc gctggaatca gcatggaagt
   115741 acatcattat ttgtactgtt ggtgtcgctt ttggtctgtt cggtaccgtg ctggtatacg
   115801 ccaacgccgc cagcgttatg ccgcaggcag aaatggcgat attctggagc gaggttctta
   115861 agcaatcgtc cttgcttgac ccaacattaa tgctgttggc ctttgtgttt gtgctaattg
   115921 gctttggtac caaaaccggg ctatttccca tgcacgcctg gctgccggat gctcacagtg
   115981 aagcgccgag tccggtcagc gccctgctct ccgccgtatt gctgaactgc gcgctgttgg
   116041 tgctgattcg ctattacatc attatttgcc aggccatcgg cagcgatttc cccaaccggt
   116101 tgttgctcat cttcggcatg ttgtcggttg ccgtggcggc atttttcatt ctggtacagc
   116161 gggacattaa gcgtctgctg gcgtactcca gcgtggagaa catggggctg gtcgcggtgg
   116221 cgctaggcat tggcgggccg ctgggaattt ttgccgcgct gctgcacacc ttaaaccaca
   116281 gtctggcaaa aacgctgctg ttctgcggtt ccggcaatgt actgctcaag tacggcacgc
   116341 gcgatctcaa cgtcgtctgt gggatgctca aaatcatgcc atttaccgcc gtgctgtttg
   116401 gcggcggtgc gctggcgctg gcgctggcgc tggcgctggc agggatgccg cccttcaaca
   116461 tttttcttag cgaatttatg accgttaccg caggactggc acgtaatcac ctgctgatta
   116521 tcgtcctgct gttattgctg ttaacgctgg tgctggcggg cctggtacgg atggctgcgc
   116581 gggtgttaat ggcgaaaccg ccgcaggccg ttaaccgggg tgatctcggc tggttgacca
   116641 cctcgccaat ggtgattctg ctggtcatga tgctggcgat gggaacgcat attccacaac
   116701 ctgtcatcag gatcctggcg ggcgcttcca ctatagtcct ctcagggacg cacgacctgc
   116761 ctgcacaacg tagcacctgg catgattttt tgccttcagg caccgcatct gtttcggaga
   116821 aacacagtga acgttaattc atcgtcaaat cgtggcgaag cgattctcgc cgccctgaaa
   116881 acgcagttcc ccggcgcggt gctggatgaa gagcgacaaa cgcctgaaca ggtcaccatt
   116941 acggtgaaaa tcaatctgct gcctgacgtt gtacattatc tttattatca acatgatggc
   117001 tggcttccag tcctgtttgg caacgacgag cggacactta acggtcatta cgcggtttat
   117061 tatgcccttt caatggaagg ggccgaaaaa tgctggatcg tggtgaaggc actggtcgat
   117121 gccgacagtc gggagtttcc gtcagtcaca ccgcgcgtcc ctgccgcggt ctggggcgag
   117181 cgagaaattc gcgatatgta cgggctgatt ccggttggcc tgccggatca gcgtcgcctg
   117241 gtgttgcccg atgactggcc ggaagatatg catccgctgc gcaaagatgc gatggattat
   117301 cgactgcgcc ctgaaccgac gacagattcc gaaacgtatg cgtttatcaa tgagggcaac
   117361 agcgatgcgc gggtgatccc tgtcggcccg ctgcatatca cctcctatga accgggtcac
   117421 ttccgcttgt ttgtggatgg cgagcaaatt gtcgatgctg attaccgcct gttttatgtc
   117481 catcgcggca tggagaaact ggcagaaacg cggatgggct acaacgaagt gaccttctta
   117541 tcggaccgcg tgtgtgggat ttgcggtttt gcccacagtg tggcctatac caactcggtt
   117601 gaaaatgcac tggggattga ggtgccgcaa cgagcacata ctattcgctc gattctgctg
   117661 gaagtcgagc ggctacacag tcatttgctc aaccttggcc tctcctgcca ttttgttggt
   117721 tttgataccg gctttatgca atttttccgc gtgcgggaaa agtcgatgac gatggcggaa
   117781 ttgctgaccg ggtcgcgtaa aacctacggt ctgaatctga ttggtggtgt tcgccgcgat
   117841 attctcaaag agcaacgtct gcaaacgctg aaactggtgc gcgagatgcg cgccgacgtg
   117901 tcggagctgg tagagatgct gcttgctacg ccgaatatgg aacaacgcac tcagggcatt
   117961 ggcattctcg accgacaaat cgcccgtgat tatagccctg tagggccgct gatccgcgca
   118021 gtggttttgc ccgtgatttg cgctttgatc acccctacgc cgactacggc aatattccaa
   118081 aaacactgtt tacctttacc ggcggcgatg ttttctcccg cgtgatggtc cgtgtcaaag
   118141 agacgtttga ttcgctggca atgctggaat ttgccctcga caacatgccg gataccccac
   118201 tgctgaccga aggctttagc tataaacctc acgcattcgc gctgggcttt gttgaagcgc
   118261 cacgcggtga agacgtgcac tggagcatgc tcggtgataa ccaaaaattg ttccgctggc
   118321 gctgtcgtgc cgccacctac gccaactggc cggtgttgcg ttacatgctg cgcggcaata
   118381 ccgtttctga cgcaccgctg attatcggta gccttgatcc ctgctactcc tgtaccgacc
   118441 gtgtgacgct ggtagatgtg cgcaagcgcc agtcaaaaac cgtgctgtat aaagagatcg
   118501 aacgctacgg cattgatcgt aaccgttcgc cgctgaagta aggacagaag atgctgaagt
   118561 tactgaaaac tattatgcgc gccggaaccg cgacggtgaa atatcccttc gcgccactgg
   118621 aggtcagccc aggctttcgc gggaaaccgg acctgatgcc cagccaatgt attgcctgcg
   118681 gtgcctgcgc ctgtgcttgt ccggcaaatg cgctgactat ccagaccgac gaccagcaaa
   118741 attcgcgcac ctggcagctc tatctggggc gttgtattta ctgcggacgt tgtgaagaag
   118801 tgtgcccgac cagagccatc cagcttacca ataactttga actgaccgtc acccataaag
   118861 ccgatctcta tacccacgcg acgttccatc tacaacgttg cagccgttgc gaacgcccgt
   118921 ttgccccgca aaaaaccgtc gcactggctg ctgaattgtt ggcacagcaa caaaatgctc
   118981 cacaaaaccg cgaaatgtta tgggcgcaag cgagcgtctg cccggagtgc aaacaacgcg
   119041 caacgctgat caacgacgat acagatgtac cgctggtggc taaggagcag ctatgagtcc
   119101 agtgcttacg caatatgtca gccagcccat cacgctggac gagcaaacgc aaaagatgaa
   119161 gcggcatttg ctacaggata tccgtcgctc ggcttacgtt tatcgcatcg attgcggcgg
   119221 ctgcaacgcc tgtgaaatcg aaatttttgc tgccatcacc cccgtattcg acgcagaacg
   119281 ttttggcatt aaggttgttt catcaccgcg tcacgccgat attttgttat ttactggcgc
   119341 agtcacccgg gcgatgcgta tgcctgcact tcgggcgtat gagtctgccc ccgatcataa
   119401 aatctgtgtt tcctacggcg cgtgcggtgt cggcggcggt attttccacg atctctacag
   119461 cgtctggggc ggcagcgaca ccattgtccc cattgatgtt tggatccccg gctgcccgcc
   119521 aacaccggcc gccaccattc acggtttcgc cgtggcgctc ggtttgctgc aacagaagat
   119581 tcacgcggtg gattatcgcg atcccaccgg ggtgactatg caaccgttgt ggccgcagat
   119641 cccgccatca cagcgtatcg ccattgagcg agaagcgcgg cggctggcgg gctatcgtca
   119701 ggggcgagaa atttgcgatc ggctcctgcg ccatttaagc gacgatccta caggaaatcg
   119761 ggttaacacc tggttgcgcg atgccgacga tccacgtctc aatagtatcg ttcagcaact
   119821 ctttcgcgta ctccgggggt tacatgactg aagagtgcgg ggaaattgtt ttctggacgc
   119881 tgcgaaaaaa gtttgtcgcc agtagcgacg agatgccgga acacagctct caggtaatgt
   119941 attactcgct ggctatcggt catcacgttg gcgtgattga ttgtctgaat gtcgccttcc
   120001 gctgcccact gacggaatac gaagattggc ttgcactggt cgaagaggag caagcccgac
   120061 gtaagatgct gggggtgatg acttttggtg agattgttat tgacgccagc cacaccgccc
   120121 tgttgacccg ggcattcgcg ccactggcag atgacgcgac gtctgtgtgg caagcgcgta
   120181 gcattcaatt cattcatctg ttggatgaaa ttgtgctgga accggctatc tatctgatgg
   120241 ccagaaaaat tgcgtgagaa ggatttctca ttaataagga ctgttgatgg ctatgtcaga
   120301 cgaagcgatg tttgccccgc cgcaaggaat aacaattgaa gcggtaaacg gaatgctcgc
   120361 ggagcggtta gcgcagaaac acggcaaggc gtctttatta cgcgccttca tcccgctgcc
   120421 gccgccgttc agcccggtac aacttattga actgcatgtt ctcaaaagca acttctatta
   120481 ccgctaccat gatgatggca gcgatgtgac ggcaacaaca gagtatcagg gcgagatggt
   120541 cgattattcg cgttacgccg tccttctcgg cagtagtgga atggcggagc tacgctttat
   120601 tcgtacccac ggcagccgtt ttactcccca ggattgcaca ctgtttaact ggctggcacg
   120661 cattatcacc ccggttctgc aatcatggct caatgatgaa gcacagcagg tggcgctgcg
   120721 tttgctggag aaagatcgcg atcatcatcg ggtactggtg gatatcacta atgcagtgct
   120781 gtcacatctt gatctcgacg atctgatcgc tgacgtcgct cgtgagatcc atcatttttt
   120841 cggtctggct tcagtcagta tggtactggg cgatcatcga aagaacgaga agttcagcct
   120901 gtggtgcagc gatctttctg cctcacattg tgcgtgtctg ccacacaata tgcctggcga
   120961 cagtgtattg ctgacacaaa cgctacaaac ccgacaaccg accttgacgc accgtgcaga
   121021 tgatctgttt ctctggcaac gcgacccgtt attactctta cttgcatcta acggctgcga
   121081 atctgcgctc cttataccgc ttacctttgg caaccataca ccgggtgcat tgttgctggc
   121141 gcatacctct tccactctct ttagtgagga aaactgccag ctactacaac acatagccga
   121201 tcgcatcgct attgccgttg gcaatgccga tgcctgccgt agaatgaccg atttgcagga
   121261 aagtttgcag caagaaaacc accagcttag cgagcagctc ctttcgaatc tgggcgtcgg
   121321 tgacattatc tatcaaagcc aggcaatgga agacctgctc cagcaggtag atattgtggc
   121381 gaagagcgac agtacggtgt tgatttgcgg tgaaaccgga accggcaaag aggtgatcgc
   121441 cagagcgatc catcaactta gcccgcgaca cgacaagccg ctggtcaaaa tcaactgcgc
   121501 tgccatcccc gccagtcttc tggaaagtga gttattcggt catgacaaag gggcgtttac
   121561 tggtgcgatt aatacccatc gtggtcgttt tgaaattgcc gatggcggca cgttgtttct
   121621 cgatgaaatt ggcgatctgc cgttagaact tcagcctaaa ctgctgcgcg tattgcagga
   121681 acgggagatt gagcgtctcg gcgggagtag aacgatcccg gtaaatgtca gagtcattgc
   121741 cgccaccaac cgtgatttgt ggcaaatggt tgaagatcgc cagtttcgca gcgatctctt
   121801 ttatcgcctg aatgtcttcc cactggaatt gcctccgctg cgcgaccgtc cggaagatat
   121861 ccctctttta gcaaagcatt tcacgcaaaa aatggcgcgc catatgaatc gttcaattga
   121921 cgccatcccg accgaggcgc tacgccagtt gatgtcgtgg gactggccgg gcaacgtgcg
   121981 cgagctggaa aacgtgattg agcgagcggt actgctgact cgcggtaaca gtctgaattt
   122041 acatcttaat gtccgacaaa gccgtttact gccgacgcta aatgaagatt cagcgcttcg
   122101 cagttcaatg gcgtagttgc tgcacccgac gacgccagag aatgacgaag aagaacgtca
   122161 gcgcattgtt caggtattgc gagaaaccaa tggcattgtt gccgggcccc gtggcgcggc
   122221 gacacgatta gggatgaagc gcaccacgct gctgtcacga atgcagcgtc tgggaatctc
   122281 ggttcgcgag gtgttgtaat ctgcttttgc aggagtatgc atgagaaaca aactctcttt
   122341 cgacttgcag ttgagcgcca gaaaagcggc aatcgctgaa cggattgccg cccataaaat
   122401 tgcccgcagt aaagtgtcgg tctttttaat ggcgatgtcc gctggcgtgt ttatggcgat
   122461 gtccgctggc gtgtttatgg cgatcggatt tactttttac ctttccgtta tcgccgatgc
   122521 cccgtcttca taggcattaa cccatctggt gggcggcctt tgctttacac ttggctttat
   122581 tttgctggcg gtttgcggca ccagcctgtt cacctcgtcg gtaatgacgg taatggcaaa
   122641 aagtcggggc gttattagtt ggcgaacttg gctgattaac gcacttctgg tggcctgcgg
   122701 taatctggca ggtattgcct gtttcagttt gttaatctgg ttttccgggc tggtgatgag
   122761 tgaaaacgcg atgtggggag tcgcgatttt acactgcgcc gagggcaaaa tgcatcatac
   122821 atttactgaa tctgtcagcc tcggcattat gtgcaatctg atggtttgcc tggcgctgtg
   122881 gatgagttat tgcggacgtt cgttatgcga caaaatcgtc gccatgattt tgtccatcac
   122941 cctgtttgtc gccagtggct ttgagcactg tatcgccaat ttgtttgtga ttccgttcgc
   123001 cattgccatt cgccatttcg cccctacctc cttctggcaa ctggcgcaca gtagcgcaga
   123061 caattttccg gcactgacgg tcagccattt tattaccgcc aatctgctcc cggtgatgct
   123121 gggtaatatt atcggcggtg cagtgctggt gagtatttgt tatcgggcta tttatttacg
   123181 tcaggaatcc tgaaaaatca gcccggcgaa acagttcgtc gggctattta tttacgtcag
   123241 gaatcctgaa aaatcagccc ggcgaaacag ttcgtcgggc tgaagattat tcttgcgcga
   123301 tttgcccatc gggccaggcg tggatcacgg ctttgatcag cgtcgccaat ggaatggcga
   123361 agaatacgcc ccagaatccc cacaaaccac cgaagatcac caccgataaa ataatcacca
   123421 gcggatgcag gttaaccgct tcggagaaca acaccggtac tagcaggttg ccgtccagcg
   123481 cctgaataat cagatacacc gcgaagcagc tccagaattc agtacctgcg ccaaactgga
   123541 atagcgccac gccaaccacc ggaatggtca ccacaaatgc gccaatgtac ggaataagaa
   123601 ccgagaagcc gaccagcact gccagcagca gcgaatagtt cagcccgaag agcaagaacc
   123661 ccagccaggt ggcgatcccc accacgatca tctccagcac tttgccgcgg atatagttgg
   123721 tgatttgttg attcatctcc ttccacacct gtcctgccag cccacggtta cgcggcagca
   123781 cccggcgaac ggcgttcagc atctgctctt tgtctttcag caggaagaag accatcaatg
   123841 gcaccagcac cagatagacg gctatggtca gcaaaccgac cagcgaggcg agggaaattt
   123901 tcaccaccga atcgcccatg gtcagcatcc gactgcgcat attttcggcc attgcatcaa
   123961 taatgcccgc gtccattaac accggatagc ggcgcggcaa cgtggcggca aagtcagaaa
   124021 gcttattgag catccccggc atatcgcgga ttaagtagat gccctgttgc caggcgatag
   124081 gcagtaccac gaacgccatc agtagcaata taccgacaaa aaccaccaat acaatcgacg
   124141 tcgcccagcg gcgggagcag ccaatagatt gcaggcgcac ggttggccat tccagcaaat
   124201 aagccagcac aatagccacc agcaacggag caagcaggcc actaaagaaa aagataatgc
   124261 caaatccggc aactaaaata accagcaagg caatcgcttc cgggtcgcta aaacggcggc
   124321 gataccattg catcaacatt tcgagcataa agaccttccc tgaacctcaa gagcgggatt
   124381 gcgatccgca attgtatcga aatgtcacaa aaaagacttc gctttttatg acggattcag
   124441 gaaactgaaa agtcatttga gtgggctaat cttcgccgtt acactcaaag gcggcgaggt
   124501 gggaacgata tttcacagta tcggtcaaat gactatcttc agaaatacag gatagaggtt
   124561 atgttcaggc agttgaaaaa aaacctggtt gcaaccctca ttgctgctat gaccattggt
   124621 caggtagccc cggcgtttgc cgacagcgca gacaccttgc cggatatggg aacctccgca
   124681 ggaagcacgc tttccattgg tcaggaaatg cagatgggcg actattatgt ccgccagcta
   124741 cgcggcagcg cgccgttaat taatgacccg ctgttaacgc aatatattaa ttcgctgggg
   124801 atgcgtctgg tttcgcatgc caattcggtt aagacaccgt ttcatttttt tctgatcaac
   124861 aacgacgaaa ttaacgcctt tgctttcttt ggcggcaacg tggtgctgca ctctgccctg
   124921 ttccgttatt ccgataacga aagtcaactg gcttcagtta tggcgcacga aatctcccac
   124981 gtcacccaac gtcacctggc gcgagcgatg gaagatcaac aacgcagcgc gccgctgacc
   125041 tgggtcggcg cgttaggttc tattttactg gcgatggcca gtccgcaggc ggggatggcg
   125101 gcgctgaccg gtacactggc gggaacgcgc caggggatga tcagtttcac ccagcaaaat
   125161 gaacaggaag cggaccgcat tggtattcag gtgctgcaac gctcgggatt cgatccacag
   125221 gcgatgccga ccttcctgga aaaattgctc gatcaggcgc gttactcctc gcgcccgccg
   125281 gaaattttat tgactcaccc gttgccggaa agtcgtctgg cagatgcccg caaccgtgct
   125341 aatcagatgc gcccgatggt ggtgcagtcg tcggaagatt tctatctggc gaaagcgcgc
   125401 acactgggga tgtataattc cggacgtaac cagctcacca gtgatttgct ggatgaatgg
   125461 gcgaaaggaa acgttcgtca gcaacgagcg gcgcaatatg gtcgtgcttt acaggcgatg
   125521 gaagccaata aatacgacga ggcgcgtaaa acgctgcaac cgttactggc ggcagaacct
   125581 ggcaacgcat ggtatctcga tctggctacc gatatcgatc ttgggcaaaa caaagccaat
   125641 gaggcaatca atcgcctgaa aaatgcccgc gatttacgca ccaatccggt gttgcagctc
   125701 aacctggcga acgcttatct gcaaggcggt caaccacaag aagcggccaa tattctgaat
   125761 cgctacacct ttaataataa agatgacagc aacggctggg atttgctggc acaggcggaa
   125821 gccgcgctaa ataaccgcga tcaggagctg gctgcgcgag cagaaggtta tgcgctcgcc
   125881 ggacgactcg atcaggccat ttcgctgttg agtagcgcca gttcgcaggt gaaattaggc
   125941 agcctgcaac aagcgcgtta cgatgcgcgc atcgaccagt tgcgccagct gcagaaacgc
   126001 tttaagcctt ataccaagat gtaattcgca ttaaaggaag aacaatgacc aaacaggtaa
   126061 aaatctacca taacccacgc tgttcaaaga gccgggaaac gctgaacctg ctgaaagaaa
   126121 acggcgtgga gccagaagtg gtgctctacc ttgagacacc cgccgatgcg gcaacgctgc
   126181 gcgatttgct gaaaatgctg gggatgaata gcgcccgtga attgatgcgc cagaaagagg
   126241 atctttataa agaactaaac ctggctgaca gttcgcttag cgaagaggcg ctgattcagg
   126301 caatggtgga taatccgaag ctgatggagc gcccgattat ggtcgcgaat ggcaaagcgc
   126361 ggattggtcg tccaccggag caggtactgg aaatcgtggg ttaagatgtg ctccatcgct
   126421 agttgaagca cattgccgga tgcgacgctt gccacgtctt atccggccta cgaatactat
   126481 ggattaactt cgcgtaggtc aggcgttcgc gccgcatccg acaataaaca ccttatctac
   126541 aacttcagaa tttctttcac aaacggaatg gtcagcttac gttgcgcggt aatcgacgca
   126601 cgatccaact gatccaacgt cataaatagc gtgcgcattt ctctgtcgag ccgcttcagc
   126661 aagaaacgcc ccacatcttc cggcagttca aaaccacgca aacgcgcgcg taactgtagc
   126721 gcctgcaact tatcttcatc agaaagtggc tgcaatttgt agatctgccc ccagtcgaga
   126781 cgcgacgcga gatccggtaa tcccagattc aactgccgcg gtggacgatc gccggtgatc
   126841 aacaaccgtg ttttgcccga ttccagaatt cgattgtaga gatcgaaaat cgccatctcc
   126901 cacaactcat cgcctgcaat acactcaatg ttgtcgatac agaccagcga caaatgctcc
   126961 ataccgtcga gcacttccgg aacaaaccag gtgcgtttat ccagcgggac atagcccacc
   127021 gcatcgccac gctgcgacaa ttccgcgcaa gccgcgtgca gcagatggct gcgccccgcg
   127081 ccttcgcgtg cccagagata gatgtaaccg ctatgttcct gacgcagcac gttttgcagc
   127141 gcggccagta aagaggagtt atcccccggc cagaaacttg caaaggtttc gtcgtcagga
   127201 agataaagtg gcaaagagag ctgtgccggt gtgttcagag atacctcaac caggatttca
   127261 caaaatcgcg agaagtttac cacagaatcc catgatgttt gaaccgggca gcaacactgc
   127321 ccggtcggta cattatttgt ctgttatatc cgcgtcttct gcgtccagca ccacttcttc
   127381 agggcgcagc acactgatca gtttgaaaat caaacttaag ccgataccga cgatggtcgc
   127441 cagcgccata ccttttaact ccgccgcgcc gatgtttacc ttcgcgccac tgacgccgat
   127501 gatcaaaatc acggaagtca ggatcaggtt ctgtgctttg ttgtaatcca ctttcgattc
   127561 gatcaaaaca cgaataccgg aagcaccaat gacaccataa agcagcagcg aaacgccgcc
   127621 catcaccggc aatgggatca tctggatagc ggcagccagt ttaccgacgc aggaaagcag
   127681 gatagcgaaa atcgccgccc cgccgataac ccaggtactg taaacacggg tgatcgccat
   127741 cacgccaatg ttttctccgt aagtggtatt tggcgtagag ccaaagaagc cggagatcac
   127801 ggtcgacagg ccgttagcaa acatcgaacg gtgcagacct ggatcgcgca gcagatcttt
   127861 tttgacgata ttagccgtta ctaccaggtg ccctacgtgt tcggcaataa ccactaacgc
   127921 cgctggcaga atagtcagaa tggcaaacca ctcgaagcgc ggcgtataga gggttggcag
   127981 tgcaaaccag tgagcattaa taatcggcgt ggtatcgaca attcccattg cgaaagagag
   128041 cgcgtacccc accagcacgc caattaaaat cgggataatt gccaggaaac cacgaaacag
   128101 cacggaacct aaaaccgtga ccgccagggt ggtaatagag atgatgatgg ttttggagtt
   128161 tggcgtttgc ccttcagccg ggagtaaacc cgccataccg gcagctacgc ccgccagctc
   128221 cagaccgatg acggcaacga ttgcgcccat tgccgcaggt ggaaacagca cgtccaacca
   128281 gccggtcccc gctttcttca cgataaaaga aaccaggcag aacagcacgc cgcacataat
   128341 aaagccgccc agcgcgactt cataccctaa cggcaacagt aacaataccg gtgaaataaa
   128401 ggcaaagctg gaaccaagat aagccggaat tttcccttta cagatgaaga gatacagcag
   128461 cgttccaata ccgttaaata acagtacagt cgccgggtta atatgaaata agacgggcac
   128521 caggacggtt gcaccaaaca tggcgaacaa atgttgcaaa ctaagcggga ttgtctgtaa
   128581 aagtggcggt ctttcactca ccccgatagc acggcgcgtc atagtattat cctctgtatt
   128641 atgtgttata ggcgctttac tcaaaaaaaa gccgactctt aaagtcggct ttaattattt
   128701 ttattcttta tttcgtacca aagattttgt caccggcatc gccgaggccc ggaataatgt
   128761 atccgtgctc gttcagtccc tggtcaatcg atgcagtata cagttcgacg tccgggtgcg
   128821 ctttttccag cgcagcgata ccttctggcg cagcgaccag caccagaact ttgatgctgc
   128881 tgcagcccgc ttttttcagc aggtcgatgg tcgcgataac ggaaccaccg gttgccagca
   128941 ttgggtcaac gatcagcgcc atacgctcat cgatgttaga aaccagtttc tggaagtacg
   129001 gtaccggctc cagcgtttct tcattacggt acataccgac aacgctgatg cgcgcgctcg
   129061 gaacgttttc cagcacaccg tccatcatac caagacccgc acgcagaatt ggcacaacgg
   129121 taattttctt acctttgatc tggtcgattt ctaccgggcc gttccagcct tcgatagtta
   129181 ctttttccgt ttcgaggtcg gcggtcgctt cgtaagtcag caggctaccc acttcggaag
   129241 cgagttcgcg aaagcgcttg gtgctgatat cttgctcacg catcagtccc agcttgtgtt
   129301 tgacgagtgg gtgtttgact tccacgatct tcatactctt tctcctttga ggggcagcca
   129361 caaaaaaatc gacggattat acctcctttc ttcagggcgg caatattatt ttcgttgact
   129421 ttagtcaaaa tgataacagt ttgagataaa gttattttat attcaaatgg ttatgaaaga
   129481 agattattcc atccgaaaac taacctttgc cctggcacaa gtcttctttc gccgcgcgcc
   129541 cggggaaaag acgtgcaaaa aggttgtgta aaacagtctc gcaaacgttt gctttccctg
   129601 ttagaattgc gccgaatttt atttttctac cgcaagtaac gcgtggggac ccaagcagtg
   129661 accgataaaa cctctcttag ctacaaagat gccggtgttg atattgacgc gggtaatgct
   129721 ctggttggaa gaatcaaagg cgtagtgaag aaaacgcgtc gtccggaagt gatgggcggt
   129781 ctgggcggct tcggtgcgct gtgtgcattg ccgcaaaaat atcgtgaacc cgtgctggtt
   129841 tccggcactg acggcgtagg taccaagctg cgtctggcaa tggacttaaa acgtcacgac
   129901 accattggta ttgatctggt cgccatgtgc gttaatgacc tggtggtgca aggtgcggaa
   129961 ccgctgtttt tcctcgacta ttacgcaacc ggaaaactgg atgttgatac cgcttcagcg
   130021 gtgatcagcg gcatcgcgga aggttgtctg caatcgggct gttctctggt gggtggcgaa
   130081 acggcagaaa tgccggggat gtatcacggt gaagattacg atgtcgcggg tttctgcgtg
   130141 ggcgtggtag aaaaatcaga aatcatcgac ggctctaaag tcagcgacgg cgatgtgctg
   130201 attgcactcg gttccagcgg tccgcactcg aacggctatt cgctggtgcg caaaattctt
   130261 gaagtcagcg gttgtgatcc gcaaaccacc gaacttgatg gtaagccatt agccgatcat
   130321 ctgctggcac cgacccgcat ttacgtgaag tcagtgctgg agttgattga aaaggtcgat
   130381 gtgcatgcca ttgcgcacct gaccggcggc ggcttctggg aaaacattcc gcgcgtattg
   130441 ccagataata cccaggcagt gattgatgaa tcttcctggc agtggccgga agtgttcaac
   130501 tggctgcaaa cggcaggtaa cgttgagcgc catgaaatgt atcgcacctt caactgcggc
   130561 gtcgggatga ttatcgccct gcctgctccg gaagtggaca aagccctcgc cctgctaaat
   130621 gccaacggtg aaaacgcgtg gaaaatcggt atcatcaaag cctctgattc cgaacaacgc
   130681 gtggttatcg aataatgaat attgtggtgc ttatttccgg caacggaagt aatttacagg
   130741 caattattga cgcctgtaaa accaacaaaa ttaaaggcac cgtacgggca gttttcagca
   130801 ataaggccga cgcgttcggc cttgaacgcg cccgccaggc gggtattgca acgcatacgc
   130861 tcatcgccag cgcgtttgac agtcgtgaag cctatgaccg ggagttgatt catgaaatcg
   130921 acatgtacgc acccgatgtg gtcgtgctgg ctggttttat gcgcattctc agcccggcgt
   130981 ttgtctccca ctatgccggg cgtttgctga acattcaccc ttctctgctg ccgaaatatc
   131041 ccggattaca cacccatcgt caggcgctgg aaaatggcga tgaagagcac ggtacatcgg
   131101 tgcatttcgt caccgatgaa ctggacggtg gcccggttat tttacaggcg aaagtcccgg
   131161 tatttgctgg tgatacggaa gatgacatca ccgcccgcgt gcaaacccag gaacacgcta
   131221 tttatccact ggtgattagc tggtttgccg atggtcgtct gaaaatgcac gaaaacgccg
   131281 cgtggctgga tggtcaacgt ctgccgccgc agggctacgc tgccgacgag taatgccccc
   131341 gtagttaaag cgccagctct gccgctggcg tttttcaatt cacctgtaaa tcgcaagctc
   131401 cagcagtttt tttccccctt ttctggcata gttggacatc tgccaatatt gctcgccata
   131461 atatccaggc agtgtcccgt gaataaaacg gagtaaaagt ggtaatgggt caggaaaagc
   131521 tatacatcga aaaagagctc agttggttat cgttcaatga acgcgtgctt caggaagcgg
   131581 cggacaaatc taacccgctg attgaaagga tgcgtttcct ggggatctat tccaataacc
   131641 ttgatgagtt ctataaagtc cgcttcgctg aactgaagcg acgcatcatt attagcgaag
   131701 aacaaggctc caacgctcat tcccgccatt tactgggcaa aattcagtcc cgggtgctga
   131761 aagccgatca ggaattcgac ggcctctaca acgagctatt gctggagatg gcgcgcaacc
   131821 agatcttcct gattaatgaa cgccagctct ccgtcaatca acaaaactgg ctgcgtcatt
   131881 attttaagca gtatctgcgt cagcacatta cgccgatttt aatcaatcct gacactgact
   131941 tagtgcagtt cctgaaagat gattacacct atctggcggt ggaaattatc cgtagcgata
   132001 gcatccgtta cgcgctgctg gagatcccat cagataaagt gccgcgcttt gtgaatttac
   132061 cgccagaagc gccgcgtcga cgcaagccga tgattcttct ggataacatt ctgcgttact
   132121 gccttgatga tattttcaaa ggcttctttg attatgacgc gctgaatgcc tattcaatga
   132181 agatgacccg cgatgccgaa tacgatttag tgcatgagat ggaagccagc ctgatggagt
   132241 tgatgtcttc cagtctcaag cagcgtttaa ctgctgagcc ggtgcgtttt gtttatcagc
   132301 gcgatatgcc caatgcgctg gttgaagtgt tacgcgaaaa actgactatt tcccgctacg
   132361 actccatcgt ccccggcggt cgttatcata attttaaaga ctttattaat ttccccaatg
   132421 tcggcaaagc caatctggtg aacaaaccac tgccgcgttt acgccatatt tggtttgata
   132481 aagcccagtt ccgcaatggt tttgatgcca ttcgcgaacg cgatgtgttg ctctattatc
   132541 cttatcacac ctttgagcat gtgctggaac tgctgcgtca ggcttcgttc gacccgagcg
   132601 tactggcgat taaaattaac atttaccgcg tggcgaaaga ttcacgcatc atcgactcaa
   132661 tgatccacgc cgcacataac ggtaagaaag tcactgtggt ggttgagtta caggcgcgtt
   132721 tcgacgaaga agccaacatt cactgggcga agcgcctgac cgaagcaggc gtgcacgtta
   132781 tcttctctgc gccggggctg aaaattcacg ccaaactgtt cctgatttca cgtaaagaaa
   132841 acggtgaagt ggtccgttac gcacacatcg ggaccgggaa ctttaacgaa aaaaccgcgc
   132901 gtctttatac tgactattcg ttgctgaccg cagatgcgcg tatcaccaac gaagtacggc
   132961 gggtatttaa ctttattgaa aacccatacc gcccggtgac atttgattat ttaatggtgt
   133021 caccgcaaaa ctctcgccgt ctgttatatg aaatggtaga ccgcgaaatc gccaacgcgc
   133081 agcaagggct gcccagtggt atcaccctga agctaaataa ccttgtcgat aaaggcctgg
   133141 ttgatcgtct gtatgcggcc tccagctccg gcgtaccggt taatctgctg gttcgcggaa
   133201 tgtgttcgct gatccccaat ctggaaggca ttagcgacaa cattcgtgcc atcagtattg
   133261 ttgaccgtta ccttgaacat gaccgggttt atatttttga aaatggcggc gataaaaagg
   133321 tctacctttc ttccgccgac tggatgacgc gcaatattga ttatcgtatt gaagtggcga
   133381 caccgctgct cgatccgcgc ctgaagcagc gggtgctgga catcatcgac atattgttca
   133441 gcgatacggt caaagcacgt tatatcgata aagaactcag taatcgctac gttccccgcg
   133501 gcaatcgccg caaagtacgg gcgcagttgg cgatttacga ctacatcaaa tcactcgaac
   133561 aacctgaata accctatgcc aatacacgat aaatcccctc gtccgcagga gtttgctgcg
   133621 gtcgatcttg gttcaaacag ttttcacatg gtcatagccc gtgtggtaga tggtgccatg
   133681 cagattattg gccgcctgaa acagcgggtg catctggcgg acggcctggg gccagataat
   133741 atgttgagtg aagaggcaat gacgcgcggt ttgaactgtc tgtcgctgtt tgccgaacgg
   133801 ctacaagggt tttctcctgc cagcgtctgt atagtcggca cccatacgct gcgtcaggcg
   133861 ctgaacgcca ctgactttct gaaacgcgcg gaaaaggtca ttccctaccc gattgaaatt
   133921 atttccggta atgaagaagc ccgtctgatt tttatgggcg tggaacatac ccaaccggaa
   133981 aaaggtcgca aactggttat tgatattggc ggcggatcta cggaactggt gattggtgaa
   134041 aatttcgaac ctattctcgt tgaaagccgc cggatgggtt gtgtcagctt tgcccagctt
   134101 tattttcctg gcggggtcat caataaagag aattttcagc gcgctcgcat ggcggcagca
   134161 caaaaactgg aaactttaac ctggcaattc cgtattcagg gctggaacgt tgcaatgggc
   134221 gcttccggta ccataaaagc cgcccatgaa gtgttaatgg aaatgggcga gaaagacggg
   134281 ataattaccc cggaacgtct ggaaaaactg gtaaaagaaa ttttacgtca ccgtaatttc
   134341 gcatcgctga gtttaccggg tctttccgaa gagcggaaaa cagtcttcgt tcctggactg
   134401 gcgattttat gtggtgtgtt tgatgcttta gccatccgtg aactgcgcct ttctgacggg
   134461 gcgttacgcg aaggcgtact gtatgaaatg gaaggacgtt tccgtcatca ggatgtgcgt
   134521 agtcgcaccg ccagcagcct cgccaaccag tatcacatag acagcgaaca ggcccgacga
   134581 gtgctggata ccactatgca aatgtacgaa cagtggcggg aacagcaacc gaagctggcg
   134641 catccgcaac tggaggcgct actgcgatgg gccgccatgc tgcatgaggt cgggttgaat
   134701 atcaaccaca gcggtttgca tcgccactcc gcttatattc tgcaaaacag tgacttgccg
   134761 ggttttaatc aggaacagca gctgatgatg gcgacactgg tgcgctatca ccgtaaagcg
   134821 attaagctcg acgatctgcc gcgctttacc ttgtttaaga agaaacagtt cctgccactg
   134881 atacagctat tgcgccttgg cgtattacta aacaatcaac gtcaggcaac caccacaccg
   134941 ccaacattga cactgattac tgatgacagt cactggacac tgcgtttccc gcatgactgg
   135001 tttagtcaga atgcgctggt actgcttgat ctggaaaagg agcaagaata ctgggaaggc
   135061 gtggctggct ggcggttgaa aattgaagaa gaaagtaccc cagaaatcgc cgcttaatgc
   135121 tttgcgggcc gacgagaaat gtcggcccgc attattcagg cactttcgcg aatgggttcg
   135181 atttcattca gcgtatcaat taacggttgc ggcttaccaa taagataacc ctgcatataa
   135241 tcgatcccca aagagagcac cgcctcgcgg atctcttcgt tttcaacgta ctctgccact
   135301 acccgcattt tcttcattcg cgccagatgg caaatcgacg ccacaatctg ataatccaga
   135361 ctatttgaca caatattgcg gataaagctg ccgtcaattt taagcagatc ggcattcaca
   135421 tttttaagcc gtgcatagct ggcgtagccg gtgccgaaat catcaatcgc aatctggcag
   135481 cctaattcct gaagatgctg caaggtgatt tgcgcctgct taacattggt cagagcatta
   135541 ctttcggtga cttcaaaaat aagttgccac gcttcaatct gatatttagc cagcaactga
   135601 ctgacttcaa caggaaaacg agcctgacat accgaggttg gagacagatt aatagcaaaa
   135661 cggtgagcgg gcatcttcgc tctgttttca gccataaatt gcagcgtatg ctcaatgacc
   135721 cacatgtcga tactcgacga taaaccaaat tcgtgcgcga ccggcaagaa gctaccgggg
   135781 ccgatcagtt catcattctc acctttcatg cgcagaagaa tttcatggta aacatcacca
   135841 cgcataccgg taatcggctg ggccatcagg aaaaaatggt tgtgttccag cgcctgctgt
   135901 agtcgattca tcatcgcgac tttatctttc aattcgcgtt gcaaatacat tgccccgcaa
   135961 cgctgcatat tttccggggc gttggtcacg atggaaagtt cggcgaccgt atttagctct
   136021 cccagcag
//
LOCUS       DAJTQQ010000002        95445 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000002 DAJTQQ010000000
VERSION     DAJTQQ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 95445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.002"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(89..1339)
                     /gene="icd"
                     /locus_tag="OO309_000132"
     CDS             complement(89..1339)
                     /gene="icd"
                     /locus_tag="OO309_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR5845430.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1511..2164
                     /gene="rluE"
                     /locus_tag="OO309_000133"
     CDS             1511..2164
                     /gene="rluE"
                     /locus_tag="OO309_000133"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR5845431.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2174..2635
                     /gene="nudJ"
                     /locus_tag="OO309_000134"
     CDS             2174..2635
                     /gene="nudJ"
                     /locus_tag="OO309_000134"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR5845432.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2689..3795
                     /gene="mnmA"
                     /locus_tag="OO309_000135"
     CDS             2689..3795
                     /gene="mnmA"
                     /locus_tag="OO309_000135"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR5845433.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3831..4472
                     /gene="hflD"
                     /locus_tag="OO309_000136"
     CDS             3831..4472
                     /gene="hflD"
                     /locus_tag="OO309_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR5845434.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4476..5846
                     /gene="purB"
                     /locus_tag="OO309_000137"
     CDS             4476..5846
                     /gene="purB"
                     /locus_tag="OO309_000137"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR5845435.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6015..6686
                     /gene="phoP"
                     /locus_tag="OO309_000138"
     CDS             6015..6686
                     /gene="phoP"
                     /locus_tag="OO309_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR5845436.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6686..8146
                     /gene="phoQ"
                     /locus_tag="OO309_000139"
     CDS             6686..8146
                     /gene="phoQ"
                     /locus_tag="OO309_000139"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR5845437.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8943..9224)
                     /locus_tag="OO309_000140"
     CDS             complement(8943..9224)
                     /locus_tag="OO309_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5845438.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9238..10899)
                     /locus_tag="OO309_000141"
     CDS             complement(9238..10899)
                     /locus_tag="OO309_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5845439.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10883..11209)
                     /locus_tag="OO309_000142"
     CDS             complement(10883..11209)
                     /locus_tag="OO309_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR5845440.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11265..11447)
                     /locus_tag="OO309_000143"
     CDS             complement(11265..11447)
                     /locus_tag="OO309_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5845441.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11431..11871)
                     /locus_tag="OO309_000144"
     CDS             complement(11431..11871)
                     /locus_tag="OO309_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5845442.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11871..12167)
                     /locus_tag="OO309_000145"
     CDS             complement(11871..12167)
                     /locus_tag="OO309_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR5845443.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(12164..12502)
                     /locus_tag="OO309_000146"
     CDS             complement(12164..12502)
                     /locus_tag="OO309_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5845444.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12499..13710)
                     /locus_tag="OO309_000147"
     CDS             complement(12499..13710)
                     /locus_tag="OO309_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5845445.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13712..14284)
                     /locus_tag="OO309_000148"
     CDS             complement(13712..14284)
                     /locus_tag="OO309_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR5845446.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14324..15481)
                     /locus_tag="OO309_000149"
                     /pseudo
     CDS             complement(14324..15481)
                     /locus_tag="OO309_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15782..16006)
                     /locus_tag="OO309_000150"
     CDS             complement(15782..16006)
                     /locus_tag="OO309_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5845447.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(16148..16309)
                     /locus_tag="OO309_000151"
     CDS             complement(16148..16309)
                     /locus_tag="OO309_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5845448.1"
                     /translation="MTAQIAAYGQLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(16319..16624)
                     /locus_tag="OO309_000152"
     CDS             complement(16319..16624)
                     /locus_tag="OO309_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5845449.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16621..16899)
                     /locus_tag="OO309_000153"
     CDS             complement(16621..16899)
                     /locus_tag="OO309_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5845450.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(17188..18936)
                     /locus_tag="OO309_000154"
     CDS             complement(17188..18936)
                     /locus_tag="OO309_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5845451.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18933..19232)
                     /locus_tag="OO309_000155"
     CDS             complement(18933..19232)
                     /locus_tag="OO309_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5845452.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(19238..19471)
                     /locus_tag="OO309_000156"
     CDS             complement(19238..19471)
                     /locus_tag="OO309_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5845453.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19473..19693)
                     /locus_tag="OO309_000157"
                     /pseudo
     CDS             complement(19473..19693)
                     /locus_tag="OO309_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19686..19907)
                     /locus_tag="OO309_000158"
     CDS             complement(19686..19907)
                     /locus_tag="OO309_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5845454.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19897..20097)
                     /locus_tag="OO309_000159"
     CDS             complement(19897..20097)
                     /locus_tag="OO309_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR5845455.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            20227..20406
                     /locus_tag="OO309_000160"
     CDS             20227..20406
                     /locus_tag="OO309_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5845456.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20531..20860
                     /locus_tag="OO309_000161"
     CDS             20531..20860
                     /locus_tag="OO309_000161"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5845457.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20910..21098)
                     /locus_tag="OO309_000162"
     CDS             complement(20910..21098)
                     /locus_tag="OO309_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5845458.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21473..22702)
                     /locus_tag="OO309_000163"
     CDS             complement(21473..22702)
                     /locus_tag="OO309_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5845459.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22951..24072
                     /gene="roxA"
                     /locus_tag="OO309_000164"
                     /gene_synonym="ycfD"
     CDS             22951..24072
                     /gene="roxA"
                     /locus_tag="OO309_000164"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR5845460.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(24121..25347)
                     /gene="pepT"
                     /locus_tag="OO309_000165"
     CDS             complement(24121..25347)
                     /gene="pepT"
                     /locus_tag="OO309_000165"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR5845461.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25597..26733
                     /gene="potA"
                     /locus_tag="OO309_000166"
     CDS             25597..26733
                     /gene="potA"
                     /locus_tag="OO309_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR5845462.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26717..27574
                     /gene="potB"
                     /locus_tag="OO309_000167"
     CDS             26717..27574
                     /gene="potB"
                     /locus_tag="OO309_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR5845463.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27571..28365
                     /gene="potC"
                     /locus_tag="OO309_000168"
     CDS             27571..28365
                     /gene="potC"
                     /locus_tag="OO309_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR5845464.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            28362..29408
                     /gene="potD"
                     /locus_tag="OO309_000169"
     CDS             28362..29408
                     /gene="potD"
                     /locus_tag="OO309_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR5845465.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29564..30385)
                     /gene="cobB"
                     /locus_tag="OO309_000170"
     CDS             complement(29564..30385)
                     /gene="cobB"
                     /locus_tag="OO309_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR5845466.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30401..31312)
                     /gene="nagK"
                     /locus_tag="OO309_000171"
     CDS             complement(30401..31312)
                     /gene="nagK"
                     /locus_tag="OO309_000171"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR5845467.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(31341..32585)
                     /gene="lolE"
                     /locus_tag="OO309_000172"
     CDS             complement(31341..32585)
                     /gene="lolE"
                     /locus_tag="OO309_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR5845468.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32585..33286)
                     /gene="lolD"
                     /locus_tag="OO309_000173"
     CDS             complement(32585..33286)
                     /gene="lolD"
                     /locus_tag="OO309_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR5845469.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(33279..34478)
                     /gene="lolC"
                     /locus_tag="OO309_000174"
     CDS             complement(33279..34478)
                     /gene="lolC"
                     /locus_tag="OO309_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR5845470.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34740..35813
                     /locus_tag="OO309_000175"
     CDS             34740..35813
                     /locus_tag="OO309_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5845471.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35941..39387
                     /gene="mfd"
                     /locus_tag="OO309_000176"
     CDS             35941..39387
                     /gene="mfd"
                     /locus_tag="OO309_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR5845472.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39530..40492
                     /gene="ldtC"
                     /locus_tag="OO309_000177"
     CDS             39530..40492
                     /gene="ldtC"
                     /locus_tag="OO309_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCR5845473.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40575..40787)
                     /locus_tag="OO309_000178"
     CDS             complement(40575..40787)
                     /locus_tag="OO309_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5845474.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            41072..41704
                     /gene="comR"
                     /locus_tag="OO309_000179"
     CDS             41072..41704
                     /gene="comR"
                     /locus_tag="OO309_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR5845475.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(41766..42305)
                     /locus_tag="OO309_000180"
     CDS             complement(41766..42305)
                     /locus_tag="OO309_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5845476.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(42532..43836)
                     /gene="ndh"
                     /locus_tag="OO309_000181"
     CDS             complement(42532..43836)
                     /gene="ndh"
                     /locus_tag="OO309_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR5845477.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(44244..44786)
                     /locus_tag="OO309_000182"
     CDS             complement(44244..44786)
                     /locus_tag="OO309_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5845478.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(44809..45834)
                     /gene="nagZ"
                     /locus_tag="OO309_000183"
     CDS             complement(44809..45834)
                     /gene="nagZ"
                     /locus_tag="OO309_000183"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR5845479.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(45845..46669)
                     /gene="thiK"
                     /locus_tag="OO309_000184"
     CDS             complement(45845..46669)
                     /gene="thiK"
                     /locus_tag="OO309_000184"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR5845480.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(46650..47291)
                     /gene="lpoB"
                     /locus_tag="OO309_000185"
     CDS             complement(46650..47291)
                     /gene="lpoB"
                     /locus_tag="OO309_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR5845481.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(47306..47683)
                     /locus_tag="OO309_000186"
     CDS             complement(47306..47683)
                     /locus_tag="OO309_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR5845482.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(47686..48045)
                     /gene="hinT"
                     /locus_tag="OO309_000187"
     CDS             complement(47686..48045)
                     /gene="hinT"
                     /locus_tag="OO309_000187"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR5845483.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            48378..50563
                     /gene="fhuE"
                     /locus_tag="OO309_000188"
                     /pseudo
     CDS             48378..50563
                     /gene="fhuE"
                     /locus_tag="OO309_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(50623..52056)
                     /gene="ptsG"
                     /locus_tag="OO309_000189"
     CDS             complement(50623..52056)
                     /gene="ptsG"
                     /locus_tag="OO309_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR5845484.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(52351..53148)
                     /locus_tag="OO309_000190"
     CDS             complement(52351..53148)
                     /locus_tag="OO309_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5845485.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(53159..54163)
                     /gene="holB"
                     /locus_tag="OO309_000191"
     CDS             complement(53159..54163)
                     /gene="holB"
                     /locus_tag="OO309_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR5845486.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(54160..54801)
                     /locus_tag="OO309_000192"
     CDS             complement(54160..54801)
                     /locus_tag="OO309_000192"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR5845487.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(54791..55813)
                     /gene="yceG"
                     /locus_tag="OO309_000193"
     CDS             complement(54791..55813)
                     /gene="yceG"
                     /locus_tag="OO309_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR5845488.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(55816..56625)
                     /gene="pabC"
                     /locus_tag="OO309_000194"
     CDS             complement(55816..56625)
                     /gene="pabC"
                     /locus_tag="OO309_000194"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR5845489.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(56745..57986)
                     /gene="fabF"
                     /locus_tag="OO309_000195"
     CDS             complement(56745..57986)
                     /gene="fabF"
                     /locus_tag="OO309_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR5845490.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(58073..58309)
                     /gene="acpP"
                     /locus_tag="OO309_000196"
     CDS             complement(58073..58309)
                     /gene="acpP"
                     /locus_tag="OO309_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5845491.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(58520..59254)
                     /gene="fabG"
                     /locus_tag="OO309_000197"
     CDS             complement(58520..59254)
                     /gene="fabG"
                     /locus_tag="OO309_000197"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR5845492.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(59267..60196)
                     /gene="fabD"
                     /locus_tag="OO309_000198"
     CDS             complement(59267..60196)
                     /gene="fabD"
                     /locus_tag="OO309_000198"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR5845493.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(60212..61165)
                     /gene="fabH"
                     /locus_tag="OO309_000199"
     CDS             complement(60212..61165)
                     /gene="fabH"
                     /locus_tag="OO309_000199"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR5845494.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(61233..62303)
                     /gene="plsX"
                     /locus_tag="OO309_000200"
     CDS             complement(61233..62303)
                     /gene="plsX"
                     /locus_tag="OO309_000200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR5845495.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(62384..62557)
                     /gene="rpmF"
                     /locus_tag="OO309_000201"
     CDS             complement(62384..62557)
                     /gene="rpmF"
                     /locus_tag="OO309_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR5845496.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(62609..63130)
                     /gene="yceD"
                     /locus_tag="OO309_000202"
     CDS             complement(62609..63130)
                     /gene="yceD"
                     /locus_tag="OO309_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR5845497.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            63329..63913
                     /gene="yceF"
                     /locus_tag="OO309_000203"
     CDS             63329..63913
                     /gene="yceF"
                     /locus_tag="OO309_000203"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR5845498.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(64025..64984)
                     /gene="rluC"
                     /locus_tag="OO309_000204"
     CDS             complement(64025..64984)
                     /gene="rluC"
                     /locus_tag="OO309_000204"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR5845499.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            65557..68748
                     /gene="rne"
                     /locus_tag="OO309_000205"
     CDS             65557..68748
                     /gene="rne"
                     /locus_tag="OO309_000205"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR5845500.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQVPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(68945..69887)
                     /gene="flgL"
                     /locus_tag="OO309_000206"
                     /pseudo
     CDS             complement(68945..69887)
                     /gene="flgL"
                     /locus_tag="OO309_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(69899..71541)
                     /gene="flgK"
                     /locus_tag="OO309_000207"
                     /pseudo
     CDS             complement(69899..71541)
                     /gene="flgK"
                     /locus_tag="OO309_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(71607..72548)
                     /gene="flgJ"
                     /locus_tag="OO309_000208"
     CDS             complement(71607..72548)
                     /gene="flgJ"
                     /locus_tag="OO309_000208"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR5845501.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(72548..73645)
                     /gene="flgI"
                     /locus_tag="OO309_000209"
     CDS             complement(72548..73645)
                     /gene="flgI"
                     /locus_tag="OO309_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR5845502.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(73657..74355)
                     /gene="flgH"
                     /locus_tag="OO309_000210"
     CDS             complement(73657..74355)
                     /gene="flgH"
                     /locus_tag="OO309_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR5845503.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(74408..75190)
                     /gene="flgG"
                     /locus_tag="OO309_000211"
     CDS             complement(74408..75190)
                     /gene="flgG"
                     /locus_tag="OO309_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR5845504.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(75328..76079)
                     /gene="flgF"
                     /locus_tag="OO309_000212"
                     /pseudo
     CDS             complement(75328..76079)
                     /gene="flgF"
                     /locus_tag="OO309_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(76099..77238)
                     /locus_tag="OO309_000213"
     CDS             complement(76099..77238)
                     /locus_tag="OO309_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR5845505.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGANNIVATTQNGYKPGDLVSYQINDDGTV
                     VGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWSATQSSGVALLGTAGTGNFGTLTN
                     GALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQDQILNTRVNLR"
     gene            complement(77263..77958)
                     /gene="flgD"
                     /locus_tag="OO309_000214"
     CDS             complement(77263..77958)
                     /gene="flgD"
                     /locus_tag="OO309_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR5845506.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(77970..78373)
                     /gene="flgC"
                     /locus_tag="OO309_000215"
                     /pseudo
     CDS             complement(77970..78373)
                     /gene="flgC"
                     /locus_tag="OO309_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(78377..78793)
                     /gene="flgB"
                     /locus_tag="OO309_000216"
     CDS             complement(78377..78793)
                     /gene="flgB"
                     /locus_tag="OO309_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR5845507.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            78948..79607
                     /gene="flgA"
                     /locus_tag="OO309_000217"
     CDS             78948..79607
                     /gene="flgA"
                     /locus_tag="OO309_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR5845508.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            79683..79976
                     /gene="flgM"
                     /locus_tag="OO309_000218"
     CDS             79683..79976
                     /gene="flgM"
                     /locus_tag="OO309_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR5845509.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            79981..80397
                     /gene="flgN"
                     /locus_tag="OO309_000219"
     CDS             79981..80397
                     /gene="flgN"
                     /locus_tag="OO309_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR5845510.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(80437..81972)
                     /gene="murJ"
                     /locus_tag="OO309_000220"
     CDS             complement(80437..81972)
                     /gene="murJ"
                     /locus_tag="OO309_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR5845511.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(82172..83005)
                     /locus_tag="OO309_000221"
     CDS             complement(82172..83005)
                     /locus_tag="OO309_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5845512.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     V"
     gene            complement(83007..83654)
                     /locus_tag="OO309_000222"
     CDS             complement(83007..83654)
                     /locus_tag="OO309_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR5845513.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(83665..84249)
                     /gene="rimJ"
                     /locus_tag="OO309_000223"
     CDS             complement(83665..84249)
                     /gene="rimJ"
                     /locus_tag="OO309_000223"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5845514.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            84485..85693
                     /gene="mdtH"
                     /locus_tag="OO309_000224"
     CDS             84485..85693
                     /gene="mdtH"
                     /locus_tag="OO309_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR5845515.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            85757..86404
                     /gene="grxB"
                     /locus_tag="OO309_000225"
     CDS             85757..86404
                     /gene="grxB"
                     /locus_tag="OO309_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR5845516.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            86538..87098
                     /locus_tag="OO309_000226"
     CDS             86538..87098
                     /locus_tag="OO309_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5845517.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            87204..88250
                     /gene="pyrC"
                     /locus_tag="OO309_000227"
     CDS             87204..88250
                     /gene="pyrC"
                     /locus_tag="OO309_000227"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR5845518.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            88324..88569
                     /gene="dinI"
                     /locus_tag="OO309_000228"
     CDS             88324..88569
                     /gene="dinI"
                     /locus_tag="OO309_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR5845519.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            88859..89113
                     /gene="bssS"
                     /locus_tag="OO309_000229"
     CDS             88859..89113
                     /gene="bssS"
                     /locus_tag="OO309_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR5845520.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            89228..90346
                     /gene="solA"
                     /locus_tag="OO309_000230"
     CDS             89228..90346
                     /gene="solA"
                     /locus_tag="OO309_000230"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR5845521.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            90394..90507
                     /locus_tag="OO309_000231"
     CDS             90394..90507
                     /locus_tag="OO309_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR5845522.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            90768..91334
                     /locus_tag="OO309_000232"
     CDS             90768..91334
                     /locus_tag="OO309_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR5845523.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            91338..91913
                     /locus_tag="OO309_000233"
     CDS             91338..91913
                     /locus_tag="OO309_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR5845524.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(91955..93007)
                     /locus_tag="OO309_000234"
     CDS             complement(91955..93007)
                     /locus_tag="OO309_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR5845525.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            93232..94152
                     /gene="lpxL"
                     /locus_tag="OO309_000235"
     CDS             93232..94152
                     /gene="lpxL"
                     /locus_tag="OO309_000235"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR5845526.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            94324..94521
                     /locus_tag="OO309_000236"
     CDS             94324..94521
                     /locus_tag="OO309_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR5845527.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            94545..>95445
                     /locus_tag="OO309_000237"
     CDS             94545..>95445
                     /locus_tag="OO309_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5845528.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFR"
BASE COUNT        22967 a        25296 c        23547 g        23635 t
ORIGIN      
        1 atatgctcga tatgctatta ataatatcca cataaataaa acaacgggcg tgttatacgc
       61 ccgtttaaat atttaacaca tgtagtgatt acatattctt gatgatcgca tcaccaaact
      121 ctgaacattt cagcagttta gcgccttcca tcagacgctc gaagtcatag gttacggtct
      181 tggcgttgat cgcgccttcc atacctttaa caatcaggtc tgcggcttca gtccagccca
      241 tatggcgtaa catcatctca gcggagagaa taatagagcc agggtttact ttgtcctgac
      301 cggcgtattt cggcgcagta ccgtgggtgg cttcaaacag ggcgcattcg tcaccgatgt
      361 ttgctccagg ggcgataccg ataccgccaa cctgcgctgc cagagcgtca gaaatgtagt
      421 caccgttcag gttcatacag gcgataacat catattcagc cggacgcagc aggatctgtt
      481 gcaggaatgc atcagcaatc acgtctttaa tgacgatctc tttgccggta ttcgggtttt
      541 taactttcag ccacgggccg ccgtcgatca gttcaccgcc aaattcttca cgcgccagct
      601 ggtagcccca gtctttaaac gcgccttcgg tgaacttcat gatgttgcct ttgtgcacca
      661 gggtcacaga gtcacgatcg ttagcaattg cgtattcgat cgctgcacga accagacgtt
      721 tggtgccttc ttcagaacac ggcttaatac cgataccgca atgttccggg aagcgaattt
      781 tcttcacgcc catctcttca cgcaggaatt taatcacttt ctcggcgtcg gcagagtcag
      841 ctttccattc gatacccgca taaatgtctt ccgagttttc acggaagata accatatcgg
      901 tcagttcagg gtgtttaacc gggcttggag tgccctgata gtaacgtacc ggacgcaggc
      961 agatgtagag atccagttcc tgacgcaggg caacgttgag agaacgaata ccgccaccaa
     1021 ccggagtggt cagcgggcct ttaatggcaa cgcgatattc acgaatcaga tcaagggttt
     1081 cagcaggcag ccagacatcc tgaccataaa cctgtgtgga tttttcaccg gtgtaaattt
     1141 ccatccagga gattttacgc tcgcctttat aggctttctc gactgcagcg tcgaccactt
     1201 tcagcatggc tggggttaca tctacaccga ttccatcacc ttcaatgtaa gggataatcg
     1261 gattttcagg aacgttgagt ttgccgtttt gcagggtgat cttcttgcct tgtgccggaa
     1321 caactacttt actttccatt cacctctcct tcgagcgcta ctggtttgct cgtctgccac
     1381 cacgttgcat atgcgtttgc gtcctgcgat acggatgctt tagagcaatt ttttgttaat
     1441 gatttgtaat tgacttgtca atactaccct attgtttgcc gtcatgaaag atgcgcgtta
     1501 ttaggctata atgcggcaat tcataatctc tgaaaatacc atgcaaaaaa cttcttttag
     1561 aaatcaccag gttaagcgat tcagctcgca acgttctacc aggcgtaaac atgaaaacca
     1621 gcccacgcgt gtgatcctct tcaataaacc ctacgatgtt cttccgcagt tcaccgatga
     1681 aaccggacgc aaaacattaa aagaattcat cccggttcag ggcgtttatg cagcaggtcg
     1741 ccttgaccgc gatagcgaag ggttgctggt gctgaccaat aacggcgcgt tgcaggcgcg
     1801 tttaacccag ccgggtaaac gcaccggaaa aatctattat gtgcaggtgg aaggtattcc
     1861 cacacaagac gcacttgaag ccttgcgcaa tggcgtaacc ttaaatgatg gccctaccct
     1921 gcccgcaggc gcggagctgg ttgacgaacc cgcgtggtta tggccgcgga atccaccaat
     1981 tcgtgaacgc aaaagtattc ccaccagctg gctgaagatc accttatatg aaggacgtaa
     2041 tcgccaggtg cgccgcatga ccgcccatgt tggtttcccc acgctgcgac tgattcgcta
     2101 tgcgatggga gattactctt tggataatct tgccaatggc gaatggcgag aagtgacaga
     2161 ttaaggatgc aaaatgttta aaccgcacgt taccgttgct tgcgtggtgc acgcagaagg
     2221 caaattttta gtcgttgaag agacgattaa tggtaaagcg ttatggaacc aacctgccgg
     2281 gcatctggaa gccgatgaaa ccttagtgga agccgccgcc cgtgaactgt gggaagaaac
     2341 cggcatcagc gcgcagccgc aacactttat tcgtatgcat cagtggattg cgccagataa
     2401 aacgccgttt ttgcgtttcc tctttgccat tgagcttgag caaatatgcc cgacccagcc
     2461 tcatgacagc gatatcgact gctgccgttg ggtcagcgcc gaagaaattt tacaggcgtc
     2521 aaatcttcgt tcgccgctgg tggcggaaag tattcgttgt tatcaaagcg ggcaacgtta
     2581 tccgctggag atgattggcg attttaactg gccttttaca aagggtgtca tctaaaaggc
     2641 tgcgtgatag aatacgccgc cttgaagttc aatgtcgtga gtgatccaat gtctgaaacc
     2701 gcaaaaaaag taatcgtcgg catgtccggc ggtgtcgatt cctccgtttc tgcctggctg
     2761 ttgcaacaac agggatatca ggtcgaaggc ctgtttatga agaactggga agaagacgac
     2821 ggtgaggaat attgcacagc agcagcggat ctggctgatg cccaggcggt ctgcgacaag
     2881 ctcggcattg aactgcacac cgttaacttt gctgccgagt actgggacaa cgtcttcgaa
     2941 ctgttccttg ccgaatataa agccggtcgc acgccgaatc cggatattct gtgcaacaag
     3001 gagatcaaat ttaaagcctt cctcgaattt gccgccgaag atttaggtgc cgattatatc
     3061 gctaccggtc attacgtacg tcgggccgat gtcgatggca agagccgcct gctgcgtggt
     3121 ctggacagca ataaagacca gagctacttc ctttatacgc tcagccatga gcagattgcg
     3181 caaagcctgt tcccggtagg cgaactggaa aaaccgcagg tgcgtaagat tgctgaagat
     3241 cttggtctgg tcaccgcgaa gaaaaaagac tctaccggca tttgcttcat tggcgaacgt
     3301 aaattccgcg agttcctggg ccgttatctc ccggcgcaac cgggcaaaat cattaccgtc
     3361 gatggcgatg aaattggtga gcaccagggg ctgatgtatc acactctcgg tcagcgtaaa
     3421 ggtctgggta tcggtggcac caaagaaggt accgaagaac cgtggtatgt ggtggacaaa
     3481 gacgtcgaaa acaacattct ggttgtcgct cagggccatg aacacccgcg gctgatgtct
     3541 gtcgggttga ttgcccagca gctgcactgg gtcgatcgcg aaccattcac cggcactatg
     3601 cgttgcacgg taaaaacccg ctatcgccag accgacattc cgtgcaccgt caaggcgctg
     3661 gacgacgatc gcattaaagt gattttcgat gagccggttg ccgccgtgac gccgggccag
     3721 tctgccgtct tctataacgg tgaagtgtgc ctcggtggcg gtattattga gcagcgtctg
     3781 ccgctgccgg tctgattatt atctttactt aaaacaagga agcagtgaac gtggcaaaga
     3841 attactatga catcaccctc gccctggccg gtatttgtca gtcggcacgc ctggtgcaac
     3901 aactcgctca ccaggggcat tgtgatgccg atgcgctaca cgtctcactc aacagtatta
     3961 ttgatatgaa ccccagctcg acgctggcgg tttttggcgg tagcgaagcc aacctgcgcg
     4021 tcgggctgga aaccctgctc ggcgtgctca atgccagcag ttgccagggc ttaaacgccg
     4081 aattaacccg ctacacactc agcttgatgg tgcttgagcg caaactctcc tcagcgaaag
     4141 gcgcgctcga cactctgggc aaccggatca acggcctgca acgccagctc gaacacttcg
     4201 atttacagtc cgaaacgctg atgagcgcga tggctgctat ctatgttgat gtgatcagcc
     4261 cgcttggccc gcgcattcag gtcaccggtt cccctgctgt actgcaaagc ccacaagtgc
     4321 aggcgaaagt tcgcgcaacc ctgctggcag gcattcgcgc cgccgtgctc tggcaccagg
     4381 tcggcggcgg acgtctacaa ctgatgtttt ctcgtaatcg cctgaccact caggcaaaac
     4441 aaattcttgc tcatttaacc ccggagttgt gatctatgga attatcctca ctgaccgccg
     4501 tttcccctgt cgatggacgc tacggcgata aagtcagcgc gctgcgcggg attttcagcg
     4561 aatatggttt gctgaaattc cgtgtacaag ttgaagtacg ttggctgcaa aaactggccg
     4621 cgcacgcagc gatcaaggaa gttcctgctt ttgctgccga cgcaatcggt taccttgatg
     4681 caattgtcgc caatttcagc gaagaagatg ccgcacgcat caaaaccatc gagcgtacca
     4741 ctaaccacga cgttaaagcg gttgagtatt tcctgaaaga aaaagtggcg gagatcccgg
     4801 aactgcacgc ggtttctgaa ttcatccact ttgcctgtac ttcggaagat atcaataacc
     4861 tctcccacgc attaatgctg aaaaccgcgc gtgatgaagt gatcctgccg tactggcgtc
     4921 aactgattga tggcattaaa gatctcgccg ttcagtaccg cgatatcccg ctgctgtctc
     4981 gtacccacgg tcagccagcc acgccgtcaa ccatcggtaa agagatggca aacgtcgcct
     5041 accgtatgga gcgccagcac cgccagctta accaggtgga gatcctcggc aaaatcaacg
     5101 gcgcggtcgg taactataac gcccacatcg ccgcttaccc ggaagttgac tggcatcagt
     5161 tcagcgaaga gttcgtcacc tcgctgggta ttcaatggaa cccgtacact acccagattg
     5221 aaccgcacga ctacattgcc gaactgtttg attgcgttgc gcgcttcaac accattctga
     5281 tcgactttga ccgtgacgtc tggggttata tcgcccttaa ccacttcaaa cagaaaacca
     5341 ttgctggtga gattggttct tccaccatgc cgcataaagt taacccgatc gacttcgaaa
     5401 actccgaagg gaatctgggc ctttccaacg cggtattgca gcatctggca agcaaactgc
     5461 cggtttcccg ctggcagcgt gacctgaccg actccaccgt gctgcgtaac ctcggcgtgg
     5521 gtatcggtta tgcgctgatt gcgtatcaat ccaccctgaa aggcgtgagc aaactggaag
     5581 tgaaccgtga ccatctgctg gatgagctgg atcacaactg ggaagtgctg gcagaaccaa
     5641 tccagacagt tatgcgtcgc tatggcatcg aaaaaccgta cgagaagctg aaagagctta
     5701 ctcgcggtaa gcgcgttgac gccgaaggca tgaagcagtt tatcgatggt ctggcgttgc
     5761 cagaagaaga gaaagcccgc ctgaaagcga tgacgccggc taactatatt ggtcgagcta
     5821 tcacgatggt tgatgagctg aaataagcct caaatcagtg ccggatggcg atgctgtccg
     5881 gcctgcttat taagattatc cactttttct tttttcactt tacctcccct ccccgctggt
     5941 ttatttaatg tttaccccca taaacacata atcgcgttac actattttaa taattaagac
     6001 agggagaaat aaaaatgcgc gtactggttg ttgaagacaa tgcgttgtta cgtcaccacc
     6061 ttaaagttca gattcaggat gctggtcatc aggtcgatga cgcagaagat gccaaagaag
     6121 ccgattatta tctcaatgaa catttaccgg atattgcgat tgtcgatctc ggattgcctg
     6181 acgaggacgg tctgtcactg attcgccgct ggcgtagcaa cgatgtttca ctgccgattc
     6241 tggtattaac cgcccgtgaa agctggcagg acaaagtcga agtattaagt gccggtgctg
     6301 atgattacgt gactaaaccg tttcatattg aagaggtgat ggcgcgaatg caggcattaa
     6361 tgcggcgtaa tagcggtctg gcttcacagg tcatttcgct ccccccgttt caggttgatc
     6421 tctctcgccg tgaattatct attaatgacg aagtgatcaa actgaccgcg ttcgaataca
     6481 ccattatgga aacgttgata cgcaataatg gcaaagtggt cagcaaagat tcgttaatgc
     6541 tacaactcta tccggatgcg gagctgcggg aaagccatac cattgatgta ctgatgggac
     6601 gtctgcgcaa aaaaattcag gcacaatatc cgcaagaagt cattaccacc gttcgcggcc
     6661 agggctacct gttcgaattg cgctgatgaa aaaattactg cgtctttttt tcccgctctc
     6721 gctgcgggta cgttttctgt tggcaacggc agcggtagta ctggtgcttt cgcttgccta
     6781 cggaatggtc gcgctgatcg gttatagcgt cagtttcgat aaaactacgt ttcggctgtt
     6841 acgtggcgag agcaatctgt tctataccct tgcgcagtgg gaaaacaata agttgcatgt
     6901 cgagttaccc gaaaatatcg acaagcaaag ccccaccatg acgctaattt atgatgagaa
     6961 cgggcagctt ttatgggcgc aacgtgacgt gccctggctg atgaaaatga tccagcctga
     7021 ctggctgaaa tcgaatggtt ttcatgaaat tgaagcggat gttaacgata ccagcctctt
     7081 gctgagtgga gatcattcga tacagcaaca gttgcaggaa gtgcgggaag atgatgacga
     7141 cgcggagatg acccactcgg tggcggtaaa cgtctacccg gcaacatcgc ggatgccaaa
     7201 attaaccatt gtggtggtgg ataccattcc ggtggagcta aaaagttcct atatggtctg
     7261 gagctggttt atctatgtgc tctcagccaa tctgctgtta gtgatcccgc tgctgtgggt
     7321 cgccgcctgg tggagtttac gccccatcga agccctggca aaagaagtcc gcgaactgga
     7381 agaacataac cgcgaattgc tcaatccagc cacaacgcga gaactgacca gtctggtacg
     7441 aaacctgaac cgattgttaa aaagtgaacg cgaacgttac gacaaatacc gtacgacgct
     7501 caccgacctg acccatagtc tgaaaacgcc actggcggtg ctgcaaagta cgctgcgttc
     7561 tctgcgtagt gaaaagatga gcgtcagtga tgctgagccg gtaatgctgg agcaaatcag
     7621 ccgcatttca cagcaaattg gctactacct gcatcgtgcc agtatgcgcg gcgggacatt
     7681 gctcagccgc gagctgcatc cggtcgcccc actgctggac aatctcacct cagcgctgaa
     7741 caaagtgtat caacgcaaag gggtcaatat ctctctcgat atttcgccag agatcagctt
     7801 tgtcggtgag cagaacgatt ttgtcgaggt gatgggcaac gtgctggata atgcctgtaa
     7861 atattgcctc gagtttgtcg aaatttctgc aaggcaaacc gacgagcatc tctatattgt
     7921 ggtcgaggat gatggccccg gtattccatt aagcaagcga gaggtcattt tcgaccgtgg
     7981 tcaacgggtt gatactttac gccctgggca aggtgtaggg ctggcggtag cccgcgaaat
     8041 caccgagcaa tatgagggta aaatcgtcgc cggagagagc atgctgggcg gtgcgcggat
     8101 ggaggtgatt tttggtcgcc agcattctgc gccgaaagat gaataaatat gtccttttac
     8161 cactacatca agtcattgat tcgatcaaac aattttcatt gaaaaagtgc caaaaatcgc
     8221 aaattgacta caccattaac tacaccgttc ggtgcactgt atgaaacaac gtggaacaaa
     8281 tagacacaag aaatatacag gcgggtaatc tttccagggg gaagcgcacc aattcatgag
     8341 gggcgttaat gtcgatatgg ggatccccat aacggggcta ccggattttt tttccggtta
     8401 acctttaatc aggctggtgg gttttacata cccttgatca ccgtaatgat gatccagatg
     8461 tgcacctttt ccaccaatgg cggaaaagct ccaaccgtaa cgatgatcgc tatggttgcc
     8521 tcgatggtct gcgtggtcat tatgaccgtt cagccagtga ccttaatcag aatttttcgc
     8581 taaaaaatga cgttggccac gtcatccgga aagcacaaaa tcacgtcgat ttttaatgaa
     8641 cggttaaacc gtctttttgt cacggtgtgt cactggttgt cactctccgt cacgattgag
     8701 gtggtggtca ttctgcccga ccgcgcatct ttgcgctatc gggaatatca aacagttacc
     8761 tcgccgactt cctcaaattg aggtttccga aaatatcaga gcgggttagc gattaagctg
     8821 acgagtaatt aaactgcgaa aatttcgtag tttctgaata tattccggcc tgctggttaa
     8881 cagtcctcag atgtgaggga tgtagccagc gcaattttgc gctttgcttt aatctcagtg
     8941 agttaccgcc gtaaccgctc cggcttcttc cagtggtacg tgattttctc cttctcccga
     9001 tacagttcaa cgcggcgatt gtaggccagc atttccagaa cgcggatccg tatgtcgcgc
     9061 atatccgcat cattaagctg gataccatca cggcgcatca cctcagcaac aacacgcgca
     9121 taattttcgg ctgtcacgct gtccggctgc gtggtctgtt cgtcagcctg ctggctgatt
     9181 ccagagacgc ggcggattaa tcgcagtatt tcggcttctg tcatgctgct gacctcatta
     9241 caccccgcta aattcttcca gtttctggcg gtggctgtcg cttatatcaa aagcaaaatc
     9301 ctcatgctct gcctggaatg taccaaacgc catcagcgcc gctacgctcg ggtctatctt
     9361 gttcggtgat tttttcttgt tcggcttgat attggcgttc gcgtcactct gcatcacgac
     9421 gttactcatc gaccaggaca acaccggatc gccacgatgc acaatcaccc tgcggttaac
     9481 aaaaacttcg aacgatttcg ccgccggact gaatctgagg taggtttgcg ggaacggctc
     9541 cacctcaaaa ccagccccct gtaattgcgt cctgagatgc gtggcgttcc acgtatcaaa
     9601 gcccaccagc ctgatattaa atttctccgc atcctgcatg atgtcatctc tgatccggtc
     9661 gtaatcaatg cagtcacctg gcgttgtgcg tatccagccc gctttagccc actggcgata
     9721 gacagcgcgg tttttattgg cggggttctg tagctggaac tccggcagat aatgacggga
     9781 aatcagcata atggttttac cgaccggaaa ggcatagcat acgctggaaa tatcgctggt
     9841 tgatgataag tccagccccg cgtaacactc ctgaccgtgt aaatcttcct ccgtgaacgt
     9901 tccggcacac tcagcccatg caccattacc catccacggg gtagtcccct gacaccagat
     9961 attaaatcgc tttgtcatca tctccaccca ctgcgacgga ataccccgcg ctttctggat
    10021 ggttgaggcc agtttttcac gatccacgga aacatcaata ttagggttcg cctttatcca
    10081 catcgccgga tcatcaacct cgttttcgtc gtccagttcg taaatcagta caaaaattga
    10141 atcgttaacc tcttcgccgt ccaggatctg gcagcaataa tcatagtgct gtttacaggc
    10201 tgaaacgacg ttactgcccg atgtggtaat ggcaaataac agcccttcgg gacgtgcgcc
    10261 catccccagc tcaagcgcgg aataaacgcc gttatcgggg tgtaggtggt attcgtccac
    10321 gatggaaaga ctggggtttg tcccctcgat ggttgccgct tttgctgcca gcggctttaa
    10381 caggctgttg cttttcgggt gtatcacctt atgcgcctga atattcaccc gcctgcgtaa
    10441 cggtcgggat aaaaggcaca tctgacgcgc attatcaaac acgatccgcg cctggtcacg
    10501 gctcaccgct gcggtgtaga tatcctgctg accgttctcc ataatcagaa accagttagc
    10561 cagaatcgcg gcggtcgtgg atttcgcatt tttgcgcggc acttcgataa aggcgctcgt
    10621 gtatttgcgc cgtccggtgg ccttaacctt aaagccgagg atgcacgcaa aggcgaactg
    10681 ctgccacggc tccagctcaa tgggtctgcc acgcatcggt ccttttacgt gcgggcacac
    10741 cctggaaaag gcaataaacc gctccacaac ctcacgatcg aacgtgtaaa gggggctttt
    10801 aaggtccgaa aagtaccgtt taacagcctg ttttaaccgt ttacaggccg gaattttgcc
    10861 cgtttttacg tcttctgcgt acttattcca ggcggtcaag ctcgtcctct tcttctgttt
    10921 ccggtggatt tttacggcgg cttatcgggt caaaaccgag caaggaggcc atttttatca
    10981 tcactctttc agcgtcggat tttgcgctta atgcggggtt tctgctctcg ccgccctgac
    11041 tgttaacaat gctgaacccg cgcgccgcaa ggtctgcaac ggctttccgg taaatggagt
    11101 agttgacgca atacagttcc agattgctcc agtcggcggg ggtcaggtct ccccgttccg
    11161 caagctgccg cgatttttcc cgccactgct tcacggcgat atcatccagg taggcggggt
    11221 cgggtttgtc ctgaaaacgc cccccacccc gtcatacagc ctcatcagcg attgcggaaa
    11281 cattccataa cctcgcggtc acggtcggtt aatcgcttcg ctgtgatgcg ttctgcgcgt
    11341 cctgacgctt tatctttatg ccctgtttcc tgtgtcttcc atgcgtcacg ctgccttata
    11401 agtccacgga taaggcggtt ttgttcccgc tcattcatca gcgtcataca tccagttatt
    11461 gcggttagca gcccgttctt cctcttcacg aaatccacct gcggcacgct tgctttttgt
    11521 tgccggatca agccatttcg ttttctggtt atgacacgcc tggcacaatg gttgatggtt
    11581 ccattcgggc cagaagagaa catcatcacc gccattaatc gggataatgt gatccaccac
    11641 cacggcgggc gtatatatcc ccttctcaag gcatcgcacg cataacgggt ttttactcag
    11701 atacatggcg cggtatttgt cccactgtct ggaatatcca cgcgcgcggc ggtgtcctcg
    11761 tctggcatcc tctgcacgcc atgccgcccg cctgtgctcc tcacacttgc cggatttaac
    11821 gcgcctgtta cagcctggtt ctgtgcatcg tcttaatggt tgccacggca tcagtacccc
    11881 cccacatcac ggtaagccgt ccagagtgcg cctatcgtca tgggtacgcg tgtttttgcg
    11941 ttatccgcga caatctggcg attttcatac aggtgagcga taaacatcat gcagccaatc
    12001 tttatggctg gcgtgaacgc cagcccgtca tcaaagcgcc tgcctatgtg tatctggcac
    12061 gcctcaagcg atgcggcaat gtatccgctg attaactggt cttcatcgtc gccatcgatg
    12121 cggcaatgga gtttcacttc ttccagggtg ataagttctt ctgtcatttt tccgcgccct
    12181 cacgacaaag aatttcaagg cgtgtcctgg cggcatccgg cagcggctgc ccgatgatat
    12241 tcagtacacg ccccgccagc ggccccgtac tgacctttat ccggctggtg gcgttgatgt
    12301 ccttccggta ccgtatccag atccttacgg taccaatcgc cagctctgcg cccgatgaaa
    12361 tggcctcctt gctgctgacc atgttcacgc ttgcccagag tgtgtgaccg tcctcccacg
    12421 tttcgagtat ttcgccggtt atatccctgg tctgtttcag ggtctgaatc gttatcctgt
    12481 cacgcagtcg ccctatgttc attccggttt ttctccctcg ctgattttta cttcctgttt
    12541 ccatgcctgg ctgaactcat caccaccatc acgcggtgac agcccttcac gttcgcgggc
    12601 ttcgttcgga cacataacgt cggattttat gccgcgctca taggtggcaa agcgttcgcc
    12661 aggtgtggcc cgtaacaggt ccgcgctgtc aaactccacc tgataccgga taccaggtac
    12721 aggcgaggcc accagcaagg cgttttttat ctgctgctca aagttcgcca gccacgggcg
    12781 catcgtcatg gtgagaaatg cgcggcttgc ctcgctgaag ttgctgtagg tgctgttgct
    12841 gtattcctgc aaaaaaatcg gcgagacgtt gaacattcgg gcgatgtctt caatggtgaa
    12901 gcgacgggag gccagccatt cggcatcctg attactcatg cccagctgct gatagctcat
    12961 ccccccttca aggatgggcg tttttccggc gtttctggcc cctttgtagc gttccagtgc
    13021 ggctaatgcc tgtttgccct tcacgccgtc cagccattcg cctgacgtga taacccctgc
    13081 cgccatcatg ccatcacgca tcacgctcgc gccgtggcgt tgttgggcca gcccaagccc
    13141 cagcgattcg cggcagatgg ttacaggtga gcgccccata aaaccgtcat ccgtggagta
    13201 acgaaggtgg agaacctccc acggtaaata gttgcggatg tttccggtgt aggcgtcagt
    13261 gatgcagtag cgccagttat gatcacctgt ctgctccacg ttcaccgact gcggcgggta
    13321 aggatgtaaa gccgccggaa aaccatcacg cccccactga ataaccgcat aagcattacc
    13381 acttaacagg cagtagcgga tcatcattcg cttaaactgg tagggtgtct gccacgcgtt
    13441 cgggcgctcg ttgaggatgt gatcgaccgg atgagaatca agccattcgc gggcctcctt
    13501 ccccttctca ttgcgtacca ggtacaggta acacggcatg gtagccaccg cgtcagagat
    13561 gaccgtgacg gcgttcatga cggcggggag tgattccgct gtcccagatg atacgtactc
    13621 acccgccccc gtgttcgatg tgccggccag cgccataaat tcatcaagcg tcatgctgcg
    13681 ctgctctttt tttcgtctga aaggccacat atcacacccc cgctaaatcc gcccaccagc
    13741 ggcgattatc cgcacgcggc attttttcgg ggtgctgctc atacagggaa cggcgggcca
    13801 gctccacgcc ggaatcgggg taagccggta ccgatgtaac ggtaatttcg tacagttccg
    13861 ccaccagcac ggtgcgcacg catggatctg ttgtggtatc ccatacatcc ttacgggagc
    13921 gaaagccaaa gctcatcccc gagatgtccc cacgcttaac cagttcgata acgtcgcgcc
    13981 ctgtactggt atccggtggg gtcagttcaa agcgtaaccc tgtttcgtcc tcttccagtt
    14041 tcagcgtgcc ggaacgggtg cgccccagta acatgctgtg gtcatgctca tacaggccgc
    14101 gaacgtcatt acccgccgca agccattcag taaacgcccc ccgctggaat ttttcgtaaa
    14161 actcacccca taacagttct gaaagcttat cccagcgaac aacgtagccc gtcagcgtac
    14221 cggctccgct ggtggtgatt tccgatgacc ggatttccat actcttcata attttttcac
    14281 ccataaaaca ctaaaggggc tttttagccc cttctgtatg ctgttaatcg tcgtcttccg
    14341 gcagtgccag aatcttgatc gcgttcgaat ccaccacgcc accgcctaaa tatttttgcg
    14401 tgaaaatttt gatgaatcct ggttctgtca ggttgtccgg tctggtgcga acacctgttt
    14461 cgtgatcaac aatgtagtaa ccgcgtttga agtcacccag ggcaataacg ttatcaggca
    14521 taaactccag atattcgacc ggaaggccca gcaacgtatc aggatcaccc gcctgtaaac
    14581 ggtcgcgcca gatataatca ccgttcgcgt tcttcacctt ctgaagtttt gccgccgtcg
    14641 tggagttaac cactcagacc gcgtttttgc ggtatttttt acgtaatgca aatttcaggt
    14701 cgatcagcgg gtccgcagat gtccacgcca gagattcgga aggtttaatt acctgcaacg
    14761 taccaaaatc acgctctttg tcgttcttct ctgcacgggg tacggataaa aaaccttttg
    14821 cttttttgtc accgtcgccc acaaccagat cgctttcttc ggtttccgtg aaggtgtcgc
    14881 caatctcacc cgtcagccat gaaaggatgt ccacatcgga aaaatccacg atttcctgtg
    14941 tggtgcgcgg gtacgcatag accggataca gcttaatgct cacctcgtta atctgcgggg
    15001 tgctggtctg ttcgcgtgcc ttaccctctt caccgtggtt aacggtcgca ccgccagcgg
    15061 aaacaagctg cttaaactcg ttgctgctga ttttcttcac ggtacagatg cgacgcatgg
    15121 tggattcatc cgccagcatt cgcatgattt cggtgttcag ttcagggata acggtataac
    15181 caccatcagc gggaacgcct gtacttaatg cgcgggtttc accggtcaga atgtagttac
    15241 gtagttctgc ggggtcagtg gtctggctgc ttttacctgg cttgctgcgc tcttcgtctg
    15301 caatggcttc aaggcgggaa atgtctttat cgagggattc agctttagcg cgtaattcgt
    15361 caaattttgc gccctcagca tcgttaagac tgcggttttc tttttcagcg ttctccagca
    15421 tgtcgcgcat ctgattttta atggcggttt tctgctggcg taattcgatt attctctgca
    15481 taaaaaaagt cctggggtta agtaaggaac tccaggacgc ggcaaacact caaccgtttt
    15541 tcataaggaa atcagtaatc gcaccgatcg ttttcccgcc tggtaatgaa tattggcgag
    15601 cacattaaca ggcgggaaag tggccccagc gtcctggcac cacgggcgag aataatcatg
    15661 attcagttcg ggtaaaatat gccgatccgc tcagtgaaca acgtggaaca accacgaaca
    15721 aataatttac aaaaaataac aaaaagccgg attgctccgg ctgtatgtgc tgtatgggtg
    15781 ctcagtctct gatccctttc agaaattcta tcaatgcatc tgtctgttca ggatttactg
    15841 ccagcatttc accggatagc gcgcaacgaa caaaaccatg atgatccttt tcaatcagcg
    15901 cccccgtttc cagaaatgcg cggtaatcag tgatattcat cgtctccatg ttgtcagcat
    15961 ggtatttatc acgctgttta agtatctcat caaatttcat cggcattgcg ttttcctctg
    16021 ttgtgtctgt ttgtttcaga tagtaactat gccggaccgt gacgaaaagc cagtagtgct
    16081 ccataccgtt tcaaatgacg caaaaaaagc cggattgctc cggctgcgtg gtgtggttct
    16141 gatattccta ctctgctaat ttgctcagcc ccatgtctgc gtaagtgcga tttactgcat
    16201 ttctcaggtc tgcgtagttc tccgttggcg gctccggtgt gcccttgctg ctctgtttta
    16261 ccagcgggtc gcccaccagc tgcccgtaag ctgcaatttg tgctgtcata ttgccacctc
    16321 agtgaagcga tccggcattg ttctttttca tctcttccat cattttttca gtgagcatta
    16381 cgcactgaaa ccccatgttt ttgatgtcta cgggtttcat gcctttgtca gtggatatca
    16441 caggcagaat atcctgatac acatcgcggt cagggtaact aagcacacaa agctcccatg
    16501 atgccacggg tgaggatcct tcgtctgttt caacccacca gcctggtgta gctgcgctta
    16561 tttgcagaat cgtgatccgt ccgttgtgct tgtaaaattt ttcaccaaat tttggttcgc
    16621 tcatgattcc acctctccgg ttttaacgtt gattgttgtt acctgttccg cttcggcaat
    16681 ctcccgctct gtcagcgtgg caaaatttgc cgccgtcgtg gtcatgaatg cgcttatcag
    16741 ttcgggatgt gctttcgcgt atccttcccc cgcgtggcgg tctatcgttc tgattgccac
    16801 ctttaaagcg tgctctgtca tgtctaacgc gcgatatttc ggttctgttc tgtctctgcg
    16861 ttttttgaat gatttattaa atttccctga agtgtgcata tttattttta ccccctcgtt
    16921 taaaaagttt tgagttgtgc ctccccttgt ctaccttatc taccttagtg gccctcatgc
    16981 cagtaatggc gctgctttca gcggggtaga gtgcttttat ccactatcta ccccgtgtct
    17041 acctccctgt ctgattcagg taaaatcagg tagagagggt agatagtggg tagacagtaa
    17101 aaaaaggcta tctacctaac ttaatgcact gaattaaatg tatttttctt tactcaggta
    17161 gacagggtag acagcaatta caaaaaatta taaaaacgcg tcgcactcgt ctgttgttat
    17221 tgcgttagtc tgcgttactc ccttaacttt ccgcgtaata tattcatgtc cgtaaacttt
    17281 cgcggctggc ttcatagcct tgccaaagtc atttacgttt agcggtttgc tcctgcctgc
    17341 gtacgccata aacgccagat agacgcggta aaggctgttt ctggtcgtgt acttcactga
    17401 atcgccaccg ccgcccatca tcaggccgcg tgcttcctcc agaaaattca ggaactggca
    17461 aaactcaata accggatccg tctgttgctt tattgccaga gcttcatcac cgtcacgctg
    17521 ttccagtagt aaagcccgtg ctttctcaga gtcggtaaag ttcgccagca atcggcggat
    17581 aatgacaggg atttcagccg caatcttttc cggtagctcc ctgtcttttt cggcctcact
    17641 aacgatattg tcgaaacgga aaatcacgcg acgacgtgcc acacctccgg cccgttcggt
    17701 gaatatcatc gggttattgt tggtcgccag caccaccgcc ctgattacag ccgtgaaacg
    17761 cttttcgtat ttcgggttaa tttccacggg gtcaccgccc gtgattttct tgatgcccgt
    17821 tccttcgcct gtatatttcg gctggtcagc caggacgata agacgactcc cgacaacctg
    17881 cgcacgtcca ccagcatcat caagcgatgt catttcagcg cttaccgtgt tctgtttccc
    17941 tgccagaagg ctggctatgt gtgtgaatgt acttttaccg ctcccgccgt ctccggtggc
    18001 ctcaataaac atctgccagt cgtaccggtt cgccataatc atgtacagcg cggcacatat
    18061 acgcatcatc ttgcgctggt cttttccggc tgcgtgctca agccatttat gaaagtttag
    18121 cgcgttatcg cggatgttct cccctggtgc tggtggcgtg tactcaatgc cgttgtgcgt
    18181 ggtgatccag ttctccggcg tgtgcgggga aaattccccc gttttcaggt caagcgcacc
    18241 attggcgaac ggcagcaaat cgccggacgg ctcgcccatt ggttcggcaa taacttttaa
    18301 cgcttccacg gcgttattga ttacgcgctt gctgaaagtg gccctgtgct ctgaatagat
    18361 caccaccatt tcgcggctaa gttccattgt gctgaccgga caccataccc cgccgcgcca
    18421 tacgtgaacg atttcacttt cctgatgcac acaaacgcca tcaaagcgct cggcaagcag
    18481 ctgcgcgcgc tcactgtctg ccatttgtgc cagttgcgta tttttctgct ccggcatggt
    18541 aagccctgcg gcaatatttt cacgctcagc attcagatag cgccgccagt tttcacactt
    18601 ctggacgtgc attccttcgg ggtaaaaatt tgcgtcctga atatctgccg ccgccagctt
    18661 ctgaccaatc tttttggtct ccaccaaatc cagttctccg gcctggtaca gccttacgcg
    18721 tttttttcca tccggaacaa ttttcagtgc atcaagttcg gcaagctgat ttggcccaag
    18781 ccacacaggc ggcacattat cgccggatgc ggggccatcc tgctcctgcc attgtttcgc
    18841 gtgcgaccag gcatcactac ccgcaaagat aattacctct gtgtctttgt gttttattcc
    18901 gcgtggctgt ttttttacgt tcggtgccag tttcattttt tacccctgaa tacgttaagc
    18961 atctttttta tttcctgaat attggcgcgt gctttctccc tgctggttgg tttactgcgg
    19021 ggcgctgcct gtaccagcgt gaaatcacgc cggaattgat aaacaggcat cacgcaatca
    19081 tattcgtaac cttcacggcg gtaggtgatg cgccgttctg ccacgccttt aatcgttacc
    19141 atgccgccgt actggtcgcg gtaaatatca ccgcgcgtaa atttagggtg agtgttgcca
    19201 ctggcagtta agccagaata tttaagtttc attattttta ttctccggtg tgttgttctt
    19261 tatatctgtc gtgcaataga tctatttctt gcagttccat tattacaggc tcaagaagcg
    19321 ttattaatgc cgtggcaatt cttgattttt gtttgtcgcg ttcattgtcg ccaagtgttt
    19381 caagccatat gcgcaatatt tccagcatgt tttcactgtg agaaagtgca agaaatgcgc
    19441 ggtctattgt ttcgtggtaa atatcacgca tattaatccc cgtccgtcgt ttttcttaaa
    19501 ataacctctg tcacgaaatc agcataatcg gcggcgatat tcagtacatc aagcccgttg
    19561 atttatattc tctcgtggaa agtaagaaaa aagccgctct aataagttct ggcattgacg
    19621 aaagcgcatc agccgcatca tcaggaacgc cggaaaattc ctgtttcagg gaattaaaac
    19681 gatcatcacg cataaccccc cccattttca caatcggcaa caagaatatt tttagcgtca
    19741 ttcagtgacc gcgttgcgat gtatcggata cattccaggg cgaactgtgt ccggtctttc
    19801 tgttcttctc cctgcgcaag ctctgccgtg cattcaatat caataagcgc gtgcatcagc
    19861 gtagtgagtg cggcggctgc tgcgttcggt gtggttttat tgtgcatagg tttccccctg
    19921 acgaatacgg gcggcgaata cagcaacaca accggacggg caacggctac gcgcttcgcg
    19981 ttccgtccaa gcggttacgt ggatgatttg ggattctccg gcactcagtg ccagaaaacg
    20041 ccacacaaag gccgtttgtg tgtgtacaag gtgtggtata tgatttacgg caaccataac
    20101 ggctcctagt ttacgttgtt ggttagaagc cccgttactg ctcctacagt gcggggcttt
    20161 gctttttgtg ttgattgtgt gtaatgtgtc actacacata aatacattac ctgaggtgta
    20221 attaacgtgt caacacacaa aaacgaaagg cgtggtaatc ctccgttcca gttccgttta
    20281 gatcctgact tacgggaaaa gatggaaaag gcacaacaac aggacggcga tgattctctt
    20341 gctgcctgga taaagcgaat aatccgcaaa gaattacagc aacgcggcat agaacccaaa
    20401 ggataaaaac tatcagcgcc gtggtgtgag gtactacggc gcattgcttt acaggcgcac
    20461 acaatgacca acgaagaatc aaccgaaaaa acatcacctg cacgaaaaag acgacgcaga
    20521 aagaaagagc atgaatcaga acgattcgcg ccgtgttctt ttgcccttga gaagtacctt
    20581 aaagaatact catcaaagga gagaacttcg cagttatggc aacgcactga accagactga
    20641 ttgcattgcc caccagcctg atagcggcta tcatccccgt gcttatgttt gggatcacac
    20701 acacaaacgg cgcagcgggt tatctgttca gaaaggcggc tccatttcgg ggccgctttt
    20761 tttatgcctg aaaaacccca attttgtggt tttccagttt caccagggcg aacgaatccc
    20821 cgcccacgtt ctggcgtata ttcaatcttc atggttatag ctctgtgttc agatgattga
    20881 tgtgtggcgg ctgcgtgccg ccagcgtgat taatgaactg ccttgcagct atccttccag
    20941 gccagaacct cggataaaga ccagccaacg gaacgaccgc caagtttacg acgtgatggg
    21001 aattgtccgg ccttttccag gcggtagcgg catgagcggc taaggcctgt tagcttttcg
    21061 cattcttttt cacgtataaa ccgatcagtg cttaacacta ttgccccctt tcgtttctta
    21121 aagagttatt tcgtgttcta ttgcgttggg atgtgtctga ttgtgtcagg atgattcaga
    21181 gttggcaaat gttgaggccg tatggtttac agaaagagga ataatcagga taaaatcctt
    21241 taaattcatg ttaatacaaa ggcataaaat attgtttcat gccttttttc tcgctattta
    21301 aagagtgatt cgctagtgta taaaaaatca gtaacacatt aaaaatcagc tacttataaa
    21361 tctgtacact ttttcacctc ttgttcgtag ttgttccgca ttgttgctca ttgttgcacg
    21421 ttgtatctgt tcgcatatcc agtatgcgca tactgaaaaa acacgaaaaa aattattttc
    21481 ttctggctac tggtagcgtg gttacgtttt catgtgttcc cgccagtatc cctaaccgct
    21541 ccgtccacat atccagcgca ttgcgtttag catccagata acgggaatga ttataaactc
    21601 gctgcattcc tggcatctgg tggcctgtaa gctgctccac gacgtgagga tccacgccta
    21661 aatcgttcag catcgttgta aaggtgcgcc ggatgtcatg tagtgaccag tgagggtgat
    21721 ttagcctcct gtgcgctaat ctgccgtact gcgacacgct tgtttcctgt ttcacttccc
    21781 ccagcaataa gcccgtgtgc ctgttctgct ccaccagctg cgtgacgaac ggcagtattg
    21841 cttccggtat gggccggaat attgccacct tcgttttgct gtgttctttc ggcacggtcc
    21901 agagcatttc ggtaaaatcc cactcgctga tctccgataa cctcagttct accgtccggc
    21961 atccgaacac aatcaggagg cggattaacg cgatgtagta gggggagaat atttttttgt
    22021 ccagtgcctg caataattcg cccagttctt tggtgcttaa gacacgctcg cttatatccg
    22081 gttttttccc aacgtccgcc acactcatat catcaagaac gttgctgatt gcatagcgcc
    22141 gcctccggca gaacttaagc gcctgtttgc acgtctgtag caagaatccg gcagtaacag
    22201 gcgttcgctt tgccacctgg tcaaaacagg ccagccagtg ccgtagctcg catttatcca
    22261 gcggcatagc accaatgtgc tgtattacgt gattattaag gcgctttttc agggcggcat
    22321 aatccacgcg gttttccttt gcgtacgact caagccagta ggtgagcgca tcgccaaccg
    22381 ttaccggctt taacgcttcc tgtacggtgt aattaagctc atgacgtgga tttttcccct
    22441 ctgccagcca tgcgcgacac tgggcggctt tttccctggc tgatttcagg ctcagatcag
    22501 gataatttcc cagcttaatg cgttccggtg gtgcccccct tcccgttccg gccctgtaag
    22561 tgaaatacca ggttaaaagg ccactggttg aatgcctgac gctcaggttt ccaccgtcat
    22621 taagaaaggc tgttttttgg gcgggtgtgc cgttgatttt cctcagctgt gtatcgctca
    22681 gtttgttaag tgctctgctc ataattttga ttccggtcat cacaattaac tacaccatta
    22741 actacaccga tcgttgcaca aagggctaca acgtgaaaca agctggaaca aggaaaatca
    22801 caaatctgtt tataatcaaa aacataatga acaatctgaa acattatgaa acggcaaaaa
    22861 acactaattg ataaaatatg tccatacttc acgcattacg ttaagcatcc gttataatcg
    22921 gttgcagata ccagcctgtg gatgcttaac atggaatacc aactcactct taactggccc
    22981 gattttcttg aacgtcactg gcagaaacgc ccggtggtgt taaaacgcgg ctttaataat
    23041 tttattgacc cgatctctcc agacgagttg gcgggtctgg cgatggaaag cgaagttgac
    23101 agtcgactgg tcagtcacca ggatggcaaa tggcaggtca gccacggccc gttcgaaagc
    23161 tacgatcatc tcggtgaaac caactggtca ttactggtac aggcagtaaa ccactggcat
    23221 gagccgaccg ccgcgctgat gcgaccgttc cgtgaactac cggactggcg tattgatgat
    23281 ctgatgattt ctttttctgt acctggcggc ggcgtcggcc cgcatctcga tcagtacgac
    23341 gtgtttatca ttcagggtac cggacgtcgt cgctggcgag tgggcgaaaa gctgcaaatg
    23401 aaacagcact gcccacatcc ggatctgtta caggtcgatc cgttcgaagc catcatcgat
    23461 gaagagctgg agcctggtga tattctttat attccgccag gattcccgca tgaaggctac
    23521 gcgctggaaa atgcgatgaa ctattccgtg ggttttcgcg cgccaaatac gcgggaatta
    23581 attagcggat ttgccgatta tgtgctgcaa cgtgaactgg gcggcaacta ctacagcgat
    23641 ccggatgttc cacctcgtgc tcatcctgcg gatgttctgc cgcaagagat ggataaactg
    23701 cgtgagatga tgctcgaatt gatcaaccag ccggaacact ttaagcaatg gtttggcgag
    23761 tttatatccc agtcacgtca tgaactggat atcgcgccgc cggaaccgcc ttatcagcca
    23821 gatgaaatct acgatgcgct gaaacaaggt gatgtgctgg tgcgcctggg tggtctgcgc
    23881 gtattgcgca ttggcgacga cgtgtatgcc aatggtgaga agatcgattc cccgcaccgt
    23941 ccggcactgg atgcactcgc cagcaacatt gcgctgactg cggagaattt tggcgatgcg
    24001 ctggaagatc cgtcattcct cgcgatgctc gcggcgctgg tcaatagcgg gtattggttc
    24061 ttcgaagggt aagtttggat ttaagccgga tgcagcatca catgccgcat cccttttcgc
    24121 ttacttccgt tgcgccgtta actcggcaat acggacgatc acctgcaccg ctttttccat
    24181 accttccaga atcacaaact catgcttacc atgatagttg taaccgccag tgaacaggtt
    24241 cgggcaaggt aatcccataa acgacaactg cgcgccgtcg gtaccgccgc ggatcggttt
    24301 cagttccggt tcaatatcgc agtcacgcat cgcctgctgg gcgatatcga gaatatgcgg
    24361 atgctcaacc actttctcgc gcatattgta gtaactgtct tcaatcacca attcaatgta
    24421 gcaatcagga tgtaaccctt tgcccacttt tttggcgatc tccatcattt tacgtttacg
    24481 cgcttcaaac tgtttacggt cgaaatcacg gatgatgtag tgcatatcgg cccgttcaac
    24541 ggtgcctttc atgctcgcca ggtgatagaa accttcatag ccttctgtca tttccgggct
    24601 ttcatccgcc ggaacttccg catgaatacg tgccgccagc gacagcgcat ttaccatcac
    24661 tcctttcgcc gtgcccggat gaacattgtt accgacaatt tttatattga ccgatgcggc
    24721 gttgaagttt tcaaactcca gttcgcctac gccaccaccg tcaacagtgt aagcccagcg
    24781 ggcatcgaag gcatcaacat caaaatgttt cgcccctttg cccacttctt catccggggt
    24841 aaaggcgacg cgaatatcac catgcggaat gtttttctgt tgcaataccg ccagcgcggt
    24901 catgatttct gcaatacctg ctttgtcatc agcacctaac aaggttttac catcggtggt
    24961 aatcaacgtc tgacccagta gctgatgcag caccgggaac ataaccggtg ataaaacttc
    25021 atcgccgata cccagcgcaa tatcgccacc gcgatagttt tcaacaattt gcggattaac
    25081 atttttgccg ctgcaatccg gtgaggtatc cacatgagaa ataaagccaa tcgccgggat
    25141 atcgccaggg acgttagccg gtaacgtcgc catcaaagtg cccttctcac ttaaggtcac
    25201 attgataagc cccatttctt cgagctgctc tttcagcaga tgcaataact tccattggcc
    25261 ttccgtgctg ggaacctgtc tcacccctgc ttttgattgg gtatccagag acacgtagtt
    25321 caaaaatcgc tcaagtagtt tatccatgta gtcaccctca ctttttgtga caacattatt
    25381 aagaagcaag aaaagacaaa tattgcgtca ggtcactttt acccctgcaa gcgggaatat
    25441 ttatcagcat taccttaatg aatataaagc taagccagta attcacaaca aagattggcg
    25501 attcaagcgg tttgccgtag aattaccgcc ctcattagga gtcaccaagg tggttaacca
    25561 caaaccccgc atcggtaagc catccgttgc gtttacatgg gacagagtaa aaaattgaat
    25621 aaacaaccga gttcgctttc accgctggtg caattggcgg gaattcgcaa atgctttgat
    25681 ggtaaagagg tcattcccca gctggatctg actatcaaca atggcgagtt cctcacgctg
    25741 cttggccctt ctggctgcgg taaaacaacc gttcttcgcc tgattgcagg tctggaaact
    25801 gttgattccg gacgcatcat gctggataac gaggacatca cccacgttcc ggcggaaaac
    25861 cgctatgtga acactgtttt ccaaagctat gcacttttcc cccacatgac cgtgttcgaa
    25921 aatgtggcct ttgggttgcg catgcaaaaa acccccgctg ctgaaattac gccccgcgtg
    25981 atggaagccc tgcggatggt gcagctggaa accttcgctc aacgtaaacc gcatcagctc
    26041 tctggtggtc aacagcaacg cgtcgccatt gctcgagcgg tggttaacaa gcctcgtctg
    26101 ttgttgctgg atgagtcgct ctcagcgctg gattacaaac tgcgtaagca aatgcagaac
    26161 gagctgaaag cgttacagcg taagcttggc attacattcg ttttcgtgac tcacgaccag
    26221 gaagaagcac tcaccatgtc ggacaggatt gtggtgatgc gcgatggtcg cattgagcaa
    26281 gacggcacgc cgcgtgaaat ctacgaagag ccgaaaaacc tgtttgttgc cgggttcatt
    26341 ggcgaaatta atatgtttaa cgccacggtg atcgagcgac tcgacgagca gcgcgtacgc
    26401 gccaacgttg aaggccgcga atgtaatatc tacgttaact tcgccgttga accggggcaa
    26461 aaactgcatg ttctgctgcg cccggaagac ttacgtgttg aagagattaa cgacgacaac
    26521 cacgctgaag ggctgattgg ttacgttcgt gagcgtaact acaaaggcat gacgctggag
    26581 tcggttgttg aactggaaaa tggcaagatg gtgatggtca gcgaattctt caatgaagac
    26641 gatcctgact ttgaccactc tctcgaccaa aaaatggcca ttaattgggt agaaagctgg
    26701 gaggtcgtac tggctgatga agaacacaag taagttccag aatgtagtga ttgtcactat
    26761 tgtcggttgg cttgtgttgt ttgtctttct gcccaacctg atgatcattg gcaccagctt
    26821 tttgacccgc gacgacgcca gtttcgtcaa aatggtcttt acactggata actacacgcg
    26881 tctgctcgat ccgctctatt ttgaagtgct attgcactcg ctgaatatgg cgctgatcgc
    26941 caccctcgcc tgcctggtgc tgggctatcc atttgcctgg tttctggcga agctgccaca
    27001 caaggtgcgt ccgctgctgc tgtttctgct gattgttccg ttctggacca actcattgat
    27061 tcgtatctac gggctgaaaa ttttcctcag caccaaaggc tatctcaacg agtttttgct
    27121 ctggctgggc gttatcgaca caccaatccg tatcatgttc acgcccagtg cggtgattat
    27181 cggtctggtt tacattctgc tgccgtttat ggtgatgccg ctgtactcca gtatcgaaaa
    27241 actggataag ccgttgcttg aggcggcgcg cgatctcggt gccagcaagt tacagacttt
    27301 tatccgcatc attattccac tgacgatgcc ggggattatt gccggatgtc tgctggtgat
    27361 gctgccagcg atgggcctgt tctatgtgtc cgacctgatg ggcggtgcga aaaacctgct
    27421 gatcggtaac gtcatcaagg tacagttcct caatattcgt gactggccgt ttggtgcagc
    27481 caccagcatt acgctgacta tcgtaatggg cctgatgttg ctggtttact ggcgcgcttc
    27541 tcgtttgctg aataagaagg tggaactcga atgatcggtc gactgcttcg cggcggtttt
    27601 atgaccgcta tctacgcgta cctgtatatc ccaatcatta ttttgattgt gaactccttt
    27661 aacagctcgc gctttggcat caactggcag ggttttacca ccaaatggta tagcctgctg
    27721 atgaacaacg acagcctgtt acaggcagcg cagcattcac taacaatggc ggtgttttcg
    27781 gcgacgtttg ctacgcttat cggttcactg acggcagttg cgctgtaccg ttatcgcttt
    27841 cgcggtaagc cgttcgttag cggaatgctg tttgtggtaa tgatgtcacc agatatcgtg
    27901 atggcaattt cgctgctggt gctgtttatg ctgctgggta ttcagcttgg cttctggtcg
    27961 ctgctgttct cgcatatcac cttctgcctg ccatttgtgg tggtgacggt gtattcgcgc
    28021 ctgaaaggtt ttgacgtgcg gatgctggaa gcggcgaaag atctcggtgc cagcgaattt
    28081 accattctgc gaaaaatcat tctgccactg gcaatgccag cggtggcggc gggctgggtg
    28141 ttaagcttta ccctgtcgat ggacgatgtg gtagtttctt cattcgtcac cggaccaagt
    28201 tatgaaattc tgccgttaaa aatttattcg atggtcaaag tcggcgtatc gccggaagtt
    28261 aacgcgctgg caaccatatt actggtgctg tcgctggtga tggttattgc cagccagctt
    28321 attgctcgtg ataaaacgaa aggtaacaca ggggacgtta aatgaaaaaa tggtcacgcc
    28381 acctgctcgc ggcgggtgct ctggcactgg gcatgagcgc cgctcacgcc gatgacaaca
    28441 acacgctgta tttctacaac tggaccgagt acgtgccgcc aggactgctt gaacagttca
    28501 ccaaagaaac cggtattaag gttatctatt cgacttacga gtcgaacgaa accatgtacg
    28561 ccaagctgaa aacgtacaaa gacggtgcct atgacctggt ggttccttca acctattacg
    28621 tcgataaaat gcgtaaagag gggatgatcc agaagatcga caagtcgaag ttaacgaact
    28681 tcagcaatct cgatccagac atgctcaaca agccttttga cccgaataac gactactcca
    28741 ttccgtatat ctggggtgcg acggcgattg gtgttaacgg tgatgcggtg gatccgaaat
    28801 ctgtcaccag ctgggccgat ctgtggaagc cagagtacaa aggcagcctg ctgttgaccg
    28861 acgatgcccg tgaagtgttc cagatggcgc tgcgtaagct gggctactcc ggtaacacca
    28921 ccgatccgaa agagattgaa gctgcatata acgagctgaa aaaactgatg ccaaacgtcg
    28981 cagcgtttaa ctccgataac ccggctaacc cgtacatgga aggcgaagtt aacctcggca
    29041 tgatctggaa cggttctgct ttcgttgcac gccaggcggg tacgcctatt gacgtggtgt
    29101 ggccgaaaga aggcggcata ttctggatgg acagcctggc gatcccggca aatgccaaaa
    29161 acaaagaagg cgcgctgaaa ttgatcaact tcctgctgcg cccggatgtg gcaaaacagg
    29221 ttgctgaaac tatcggttat ccaacgccaa accttgcggc gcgtaagctg ttaagtccag
    29281 aagtggcaaa cgataagacg ctctacccgg atgctgaaac cattaaaaat ggcgaatggc
    29341 agaatgacgt tggcgcagcc agcagcattt atgaagagta ttatcagaag ctgaaagcag
    29401 gacgttaatt cagcaagcgg gcggtggtat taccgcccgt tttttagtta gcaaggatgt
    29461 ggctaatgag tcaggacagt aaagttttat cccgctcttt cgtcggtatt gtggtccgct
    29521 tgtaggcctg ataagcgtag cgcatcaggc aatgcttcct gctttataat ccctccagca
    29581 acttttcaac aaattctggc accacctggc ttgccgggcc gtaatatttc tcggcaaatt
    29641 cattaccaac ctgactcggt tcaagattca gttcaacggt gtgagcacca tgtagtttgg
    29701 cttcgtgtac aaacccagcc gccggataga catgcccgga agtaccaatg gcaataaaga
    29761 catccgccat cgacaacgcc atataaattt catccatgcc gagtggcatt tcgccaaacc
    29821 aaacgacgtg cgggcgcaag ggtgccggga actggcaaca atggcattta tcttctggcg
    29881 taacgtctcc ggtccagtcg agaacctgac cactttgcga acaacgaact ttcagcaatt
    29941 ccccatgcat atgaatgaca ttggtattac ctgcgcgttc atgcaggttg tctatattct
    30001 gcgttaccag caaaaagcga tcgccgagag cgtcttgcag tttagccagc gcaagatgcg
    30061 cggcgttagg ctgaatttct ggctgctgca gctgtcgacg acgggcgtta taaaacgctt
    30121 gcaccagttc aggatcgcga tcgaaacctt ccggcgttgc cacatcttca acccgatgtt
    30181 cttcccacag gccatctgcg gcgcgaaagg tacgaatacc tgattccgca gaaattcctg
    30241 cccctgtcag tacgagtact cttggttttt ccattgcttc cggcaccact ttatctctga
    30301 aaaaaatacg ctgacgcaaa cgctcgcgca ggcgacgttt atttttacga aaacgactta
    30361 accgatgacc ccgacgcgac agcatagcaa cctctgttgt ttaatcggtt agatgtagga
    30421 aggccgcacc acgcattcct cccgcatcac cgtggcgcgc gcgttcaatg cgtggaacac
    30481 gagctacagg taagagatga caaggcaacc tgtccgccag ttgcgttgtg attgccggga
    30541 aattcgataa gccaccacca atgacgacca ggtcagggtc aacaatggtc aggatatttc
    30601 ccagagaaac cgctaataaa tccagataac gctcaacgtg cgcccttgcc tgctcatcgc
    30661 cttgatcata aagcgcaata atttcgggag cctgcaacgg ttgatgataa tagtgttgat
    30721 acagccacgc aaaaccgcga ccagacagat aattttcaat gcagccatgc tgaccacagc
    30781 cgcagcggcg taacgggaaa tccaacccca tcatggttaa cgcatcaacc ggcagacgca
    30841 tatggccaaa ctcgccggta atgtagcttt taccagtaat cggtttgccg ttgaaaacca
    30901 gcccgccgcc aacgccggtg ccgagaatta accccatcac cagtggatat tgcgtaaatt
    30961 catcatccca ggcttctgaa agggcaaaac agttggcatc gttatcaagg cgtacatcgc
    31021 gatcaagacg tgcgctcagg tcggcacgca gcggtttgcc gctggcagcg gggacattag
    31081 cggcatacag cttaccatct tctgtttccg gcattcccgg aataccgatg ccgacagaac
    31141 ctttacagcc aaaacgttgg tcagcttcag ctaccagctc acacactgca tctaaaaatg
    31201 cgtcatagct gtcacgcggt gtcggcaccc gcttttccca ctgcaactgc cgaccgctat
    31261 caaacacgcc aagcgcaatt tttgttccac caatatcaaa cccgtaatac attgccgctc
    31321 cttgttttaa tgtactgcct ttactggccg ctaaggactc gcgcagggtc aatattactg
    31381 gcgcgccgcg ccggatacca acttgccaaa agactcagca acaatgctgt gaccagtacg
    31441 tagaagacgt ccagccagtg caattccgat ggcaggaagt caataaaata aatatcgctg
    31501 gagaggaact gatgaccgat cagcttttca atccactcaa taatcggggt aagttgcagt
    31561 gaaacaacta cgccgataac caccccacac aggctgccga acagccctgc cagcaatcca
    31621 taccagacaa agatggcgcg aattaaacca tctttcgccc ccagcgttct taatactgcg
    31681 atatcgccac tcttgtcttt caccgccatc actaaggtgg agacgatgtt gaaacaggcc
    31741 acgccaatca ccagtaccat cgccagatac ataatggcgc ggatcatctg gatatcgcga
    31801 tacatatagc cgtaagtacc aatccaactt ttaatataaa catagctgtt ggtcacttca
    31861 cccgcatcgc gtaccagctt attggcgttg aaaacatccg tcattttaag ggcaatacct
    31921 gacacgctgg aacccatatc aagatattgt tgggcatccg ccagcgggat catggcaaaa
    31981 ctgtgatcga gttgaccact caactgcaaa ataccggcaa tgtgcaaacg cacacgtttt
    32041 ggctgcatca gtttatgctc gggattcgag ttggggatca tgatcgacac ccaatcgccc
    32101 tgcttcactt tcagcgcatc cgccacgcct ttgccgatga taatttgctg ttcgcccgct
    32161 ttaaaattgc gccacgcatc cccctgaaca aacgagggta atgcgctcag acgctgttcc
    32221 tgttgtgggt taacgccctt cacctggatt gcgcgcagat tcgctccact ttccaccaga
    32281 ccggtgaaat tgatatacgg cgcagcggcg gcaatacccg gcactttctg cacgttatcc
    32341 agtgcttcct gccagttagt ccatggctga tttaccgctt cgatttcgcc atgcggcacc
    32401 accgccagaa tgcggttatt cagttcgcgt tcaaagccgt tcatcgcgct taagccgacg
    32461 atcaataccg ccacgccgag ggcaatgcca atggtagaaa tgacagagat tagcgacacc
    32521 atgccgccgc gtcgccgtcc gcgactaaaa cgcaggccaa tcaataacga taaaggcatc
    32581 gccattactc cgcccccatc aggctcagtt ccgccgtcag acgtccatca cgcatttcca
    32641 gttggcggct catacgtttc gccagttgca ggtcgtgagt gaccaccagg aaagcggtgc
    32701 cctgcaagcg attcaactcc ccaagcaact ggaagatgct gtcggcgtta cgcgcatcga
    32761 ggttaccggt aggttcatcc gccagtacca gacgcggatt attgaccagc gcacgggcaa
    32821 tcgctacacg ctggcgttcg ccgccagaaa gttcagatgg gcggtgattc gcacgatgct
    32881 ccagccccac cgcttttaac atctccagtg cacagctgtt gatttcagcg ggctttttct
    32941 tgccaatcag cagtggcata gccacgtttt ccagggcagt aaaatccggc agcaggtggt
    33001 gaaactgata aataaagccc agcttctggt tgcgcagttc tgctttcgcc gctgaagaca
    33061 gtttgctcat cggctgaccg ttaaagatca catcgccgga ggttggcgta tccagcccgc
    33121 ctaacaggtg cagcaaggta cttttaccgg aaccagagct accgacaatt gccatcattt
    33181 caccttcgcc cacgctgaaa ctgacattga gcagcacatc ggtttgcaca ctgccttcct
    33241 gatagcgttt gcacaggttg tcgcattgca acaggatctt attcataacg taaagcctca
    33301 gcgggttgtg tggcggcagc gcgccatgaa gggtaaagcg tagacagcag cgcgatagcc
    33361 atcgccacca gcgcaataac aatgacctgt aaaggttcga tagccaccgg cagcgccgcg
    33421 ccatcaagca ggacgccgat tatcggcatc agattattta actggctggc aagcagtgcg
    33481 ccaagcgccg ctccgaggat tgcaccgata atcccggcgc tagccccttg caccataaag
    33541 accatcatga tttgtcgcgg agttaagcct tgcgtttgca ggatcgcgac ttcgccctgc
    33601 ttctccatca ccatcagccc cagcgaggta ataatgttaa acgccgcaac agcgacaatc
    33661 aggctcagca gtaagcccat catatttttt tccatgcgta cggcctggaa cagctcgcct
    33721 ttacggtcac gccagtcctg ccatttgctg ccttcaggca gtttttgctg acttaaagag
    33781 tcaactctca gcggctcatc cagccacaaa cgccagccgg taatattggc tgccggataa
    33841 cgcatcagac gcgaggcatc ctcaatattc accagcattt catagccatc gacttcactg
    33901 ttggcggcga aagtgccaat cacattgaac aggcgctgac ttggaatacg ccccatcggc
    33961 gtgaactggc tggcagatgg caccatcacg cggatttgat caccgcgatt gacgcccaac
    34021 tgtgaggcaa gttgttcgcc gaggatgaca ttatatttcc ccggctcgag gtcagtttgt
    34081 ttcacattga ccagatacgg cgttagtgga tctttttgcg ccgggtcgat accgagcatc
    34141 accccgaccg ccacgctgcg cgcgctttgc agtaccacat caccggtagt aataggtgcg
    34201 acgcgattaa cgccgtccag tttgactgcc gtttccggga gttgctgcgg gttaagagag
    34261 ccatgctcag aagagagaat tgcctgtggc atcaggccaa ggatgttgtt ttgcagctcg
    34321 cgctcaaagc cgttcatcac tgacaatact gtgaccagcg ccatcacccc gagggtaatg
    34381 ccgatggtag aaagccagga gacgaaacga ccgaagcgat ccactgcacg cccacgcatg
    34441 taacgcaggc caataaatag agcgacaggt tggtacatga aatccgtctg gttgctgtag
    34501 caaagacacg agtatataag cgaaagcaag atgggtaaac gtctgaactg taagtgatcg
    34561 tcagggctaa tctgaaaagc ccccgtaaat caatcggcta aattgccata ttttatcatc
    34621 aatcttgccg cctgatctta atctgaaaaa tcatccaata cagacagata cccattacac
    34681 ctcgggtcat aaccagcagg atcaaccgca tgtaaattaa aaaagagcgt gttgttctga
    34741 tgaaacaaaa agagctatgg attaaccaga tcaaagggtt atgtatttgt ctggtggtga
    34801 tttatcactc ggtcattacc ttttatccgc atatgaccac ttttcagcat ccgttatcgg
    34861 aagtcctgag caaatgctgg atctatttca atctttacct tgcccccttt cgtatgccgg
    34921 tttttttctt tatatccggc tatttgattc gccgctatat cgacagcgta ccgtggggaa
    34981 attgtctcga taaacggatc tggaacatct tctgggtgct ggcgctttgg ggcgtggtgc
    35041 agtggctggc gttaagtgcg ctgaaccagt ggctggcacc tgagcgcgat ttaagcaatg
    35101 cctccaatgc cgcttatgcc gattccaccg gtgagttcct gcacgggatg atcaccgcca
    35161 gcaccagctt gtggtatctg tatgcgttaa ttgtctattt cgtggtatgt aaaattttta
    35221 gccgtctggc gctgccacta ttcgccttgt ttgtactgct gagtgtggcg gttaatttcg
    35281 ttcccacgcc gtggtgggga atgaacagtg tgatacgcaa tttgccttat tacagccttg
    35341 gcgcatggtt tggcgcaaca ataatgacct gtgttaaaga ggtgccgttg cgccgccatc
    35401 tgctgatggc ttctttgctt accgttctgg cggtcggtgc ctggttgttt actatctcgc
    35461 tgctgttgtc gctggtatcg attgtggtga tcatgaagct gttttatcag tacgagcaac
    35521 gattcggtat gcgctccacc agcctgctga atgtgattgg ctccaacacc attgctatct
    35581 acaccaccca tcgcattctg gttgaaatat tcagcttaac tctgcttgcg caaatgaacg
    35641 cagcacgctg gtcgccgcaa gtcgaactga cactcctgct ggtttatccc tttgtcagtt
    35701 tgttcatctg tactgttgcg ggcttgctgg taagaaaact ttcacagcgt gcattcagcg
    35761 atctgttgtt ctccccgcct tcactgcccg ctgccgtcag ttactctcgt taaaccatca
    35821 ccccagccgg ggcgattcaa tttgctaaac catgtcgatt acggataatg aacagcattg
    35881 cttatcagag atatgccccc atatgttgag gcatatccta acgagaatct gacaaccgtt
    35941 atgcctgaac aatatcgtta tacgctgccc gtcaaagcgg gtgagcagcg tctgctgggc
    36001 gagttaaccg gcgcagcctg tgcaacgctg gtagcggaaa ttgccgaacg tcacgccggt
    36061 ccggtggtac tcattgcacc agatatgcaa aatgctctgc gtttgcatga tgaaatcagc
    36121 cagttcaccg atcagatggt gatgaatctg gcggactggg aaactcttcc ctacgacagt
    36181 ttttcgcctc atcaggacat tatctcctcg cgcctttcca ccctttacca gctaccgacg
    36241 atgcagcgtg gcatactgat tgttccggtg aatacgctta tgcagcgcgt ttgcccacac
    36301 agttttctcc acggtcatgc gctggtgatg aaaaaaggtc agcgcctgtc acgagatgca
    36361 ttacgaaccc aactggacag cgccggttat cgccatgttg accaggtgat ggagcacggc
    36421 gaatacgcca cgcgcggcgc gttgctggat ctcttcccga tggggagtga gctgccttat
    36481 cgtcttgatt tctttgatga tgaaatcgac agcctgcggg tgtttgacgt cgacagccag
    36541 cgcacgctgg aggaagtaga agcgatcaat ctgctgcccg cgcacgaatt tccgaccgat
    36601 aaagcggcaa ttgaactgtt ccgcagccag tggcgcgata ccttcgaagt gaagcgcgat
    36661 ccggaacata tttaccagca agtgagtaaa ggcacattac ctgctgggat cgagtactgg
    36721 cagccattgt tcttcagcga accactgcca ccgctgttca gttatttccc tgccaatacc
    36781 ttgctcgtga atactggcga tctggaaacc agtgccgaac gtttccaggc tgacacgctg
    36841 gcgcgttttg agaatcgcgg cgtcgatccg atgcgcccgc tgttgccacc acaatcgctc
    36901 tggctgcggg tggacgagct cttctcagag ctgaaaaact ggccccgagt gcagctaaaa
    36961 actgaacatt taccgacaaa agccgcgaat gccaatttag gtttccagaa actgccagac
    37021 ctggccgttc aggcgcaaca aaaagcgccg ctggatgcgc tgcgtaagtt cctcgagact
    37081 ttcgacggtc cggtggtgtt ctcggtagaa agtgaaggtc gccgtgaagc gctgggtgaa
    37141 ctgctcgcac gaattaaaat tgctccgcaa cgcattatgc gtcttgatga agccagcgac
    37201 cgtgggcgtt atctgatgat tggcgctgcc gaacatggtt ttgtcgataa ggcgcgtaat
    37261 ctggcgctga tctgcgaaag cgatctgctc ggtgaacgtg ttgcccgccg tcgtcaggat
    37321 tctcgccgca ccatcaaccc cgatacactg atccgtaacc ttgcggagct gcatattggt
    37381 cagccggtgg tccatctgga gcacggcgtc ggtcgctatg ccggaatgac cacgctggaa
    37441 gctggcggca ttactggcga gtacctgatg ctcacctatg ccaacgacgc caaactgtat
    37501 gttccggtgt cgtcactgca tctgattagc cgttacgcag gtggcgcgga agaaaacgcc
    37561 ccgctgcata aacttggcgg cgatgcgtgg tcacgcgcgc ggcagaaagc ggcggaaaaa
    37621 gtgcgtgatg tggcggcgga attgctggat atctacgcgc aacgcgccgc caaagagggc
    37681 ttcgcgttta aacacgatcg tgagcagtat cagttattct gcgacagctt cccgtttgaa
    37741 accacgccgg atcaggcgca ggccattaat gcggtactta gcgacatgtg tcagccgctg
    37801 gcaatggatc gtctggtgtg cggcgatgtt ggctttggta aaacagaagt ggcgatgcgc
    37861 gccgctttcc tggcagtaga taaccacaag caggtagcgg tgttggtgcc taccaccctt
    37921 cttgcgcagc agcattacga caacttccgc gaccgtttcg ccaactggcc ggtacgtatc
    37981 gaaatgatct cccgtttccg cagcgccaaa gagcagacgc aaatccttgc ggaagtggcg
    38041 gaagggaaaa tcgatattct gatcggtacg cacaaactgc tgcaaagtga cgtcaagttt
    38101 aaagatttag gcctgctgat tgtcgatgaa gaacaccgct tcggggtgcg tcataaagag
    38161 cgcattaaag cgatgcgcgc gaacgtggat attctgacgc ttactgcaac gccgatccca
    38221 cgtacgctga atatggcaat gagcggaatg cgtgacctgt cgattatcgc cacgccgccc
    38281 gcccgtcgtc tggcagttaa aacctttgtc cgtgagtatg acagcctggt ggtccgggag
    38341 gcgatcctgc gtgaaatttt gcgcggagga caggtttatt atctctacaa tgatgtggaa
    38401 aacattcaga aagccgccga acggctggca gaactggtgc ctgaagcacg gattgccatc
    38461 ggtcacgggc agatgcgcga gcgcgaactg gaacgggtga tgaatgattt ccatcatcaa
    38521 cgtttcaacg tgctggtttg taccaccatt atcgaaaccg ggatcgacat cccgacagcc
    38581 aacaccatta tcattgaacg tgcggatcac ttcggtctgg cgcagctgca ccagttacgc
    38641 ggtcgcgtcg gacgttcaca tcatcaggca tatgcatggc tgctgacgcc gcatccaaaa
    38701 gcgatgacta ccgatgcaca aaaacgtctt gaagcgattg cctcgctgga agatctcggg
    38761 gcaggttttg cgctggcaac gcacgatctg gagattcgcg gcgcgggtga actgcttggc
    38821 gaagaacaaa gcggctcaat ggaaaccatc ggtttctcgc tgtatatgga gttgctggaa
    38881 aacgccgtcg atgcactgaa agccggacgc gagccgtcgc tggaagatct caccagccag
    38941 caaacagaag tcgagctgcg gatgccgtcg ctattgccag atgatttcat ccctgacgtg
    39001 aacacgcgtc tgtcgttcta caaacgtatt gccagcgcca aaacggaaaa cgaactggaa
    39061 gagatcaaag tcgagcttat cgatcgcttc ggcctgctgc cagatccggc gcgtaccctg
    39121 ctggatattg cccgtctgcg ccagcaagcg cagaaactgg ggatcaggaa gctggaaggt
    39181 aatgagaaag gcggggtgat cgaatttgcc gagaagaatc acgttaatcc ggcctggttg
    39241 attggtttgc tgcaaaaaca gccgcagcat taccgccttg atggtccgac gcgcctgaaa
    39301 tttattcagg atttgagtga gcggaaaacg cgtatcgaat gggtacgcca gtttatgcgt
    39361 gaactggaag agaacgcgat cgcttaatgt aaatcggggg cgtttctgcg cccccatctg
    39421 caacatttac aaattctttg cactttcctg cactatccga cactgtcacc atccataatt
    39481 caggcttatc tgtttattac aataacctta tatttattat gtatttttgg tgatgatcaa
    39541 aacgcgtttt tctcgctggc taacgttttt tacgttcgcc gctgccgtgg cgctggcgct
    39601 accggcaaaa gccaacacct ggccgctgcc gccagcgagc agtcgtctgg ttggcgaaaa
    39661 caaatttcat gtggtggaaa atgacggtgg ttctctggaa gccatcgcca aaaaatataa
    39721 cgtcggcttt ctcgctctgt tacaagctaa ccccggcgtt gatccttacg taccgcgcgc
    39781 gggcagcgtg ttaacgatcc cgttgcaaac cctacttcca gacgcgccgc gcgaaggcat
    39841 tgtgatcaac attgcggagc tgcgtctcta ttactacccg ccgggtaaaa attcggtaac
    39901 cgtgtatcca ataggtattg gtcagttagg tggtgacacg ctgacaccga caatggtgac
    39961 caccgtttca gacaaacgtg caaacccaac ctggacgcca acggcaaaca tccgcgcccg
    40021 ttataaagca cagggaattg agttgcctgc ggtagtgccg gctggaccgg ataacccaat
    40081 gggccatcat gcgattcgtc tggcggccta tggcggcgtt tatttgcttc acggtacgaa
    40141 cgccgatttc ggcattggca tgcgggtaag ttctggctgt attcgtctgc gggatgacga
    40201 tatcaaaaca ctctttagcc aggtcacccc aggcaccaaa gtgaatatca tcaacactcc
    40261 gataaaagtc tctgccgaac caaacggtgc gcgtctggtt gaagtacatc agccgctgtc
    40321 agagaagatt gatgacgatc cgcagctgct gccaattacg ctgaatagcg caatgcaatc
    40381 atttaaagat gcagcacaaa ctgacgctga agtgatgcaa catgtgatgg atgtccgttc
    40441 cgggatgccg gtggatgtcc gccgtcatca agtgagccca caaactctgt aaatcctgga
    40501 taaaaaaaat ccccgcgaca cggcatgtcg cggggaaaaa atcagcagta gcaggcatta
    40561 atgagggtta atgcttattt ataaattact gctgttccat ggagggtatt cggaccggtt
    40621 acagaagtaa tacggaaaga ttttgcgccc atctcatccg ctttttgcgc cagctgctct
    40681 tccagcgatc ccagatttgt ccccgcgtta gcactgatag taccgacttt ttgttggcct
    40741 tctggcgttg actgaacttc gacagccgca aagttggcaa atgacatgga gcttaaaatc
    40801 gccgcagcga tgaggttttt acgtttttca taatagtggc cttatgcaga tgaatgacgt
    40861 cgcaagaaat tacttaacga tcgataacta aatgatagat gtgatctgga tcacatacaa
    40921 gatatttttt ataacaatca ttaattaatt taaaaatctt taaatttcag tcaaatagaa
    40981 agcaattatt tttatgggat ctggactggt gaacggaggt gattattttt ataataacct
    41041 ttcattcaca gtcaccaggt acaacggcat catggcaact gactcaacac aatgtgtaaa
    41101 aaaaagccgt ggccgcccaa aagtgttcga cagggatgcc gcgcttgata aggccatgaa
    41161 attgttctgg caacacggtt atgaagcgac ttctcttgcg gacctcgtcg aagcaaccgg
    41221 agccaaagcg cccacgctat acgcggaatt taccaacaaa gaggggttat ttcgtgccgt
    41281 tctcgaccgc tatatcgatc gttttgccgc taagcatgaa gcacagctgt tttgtgaaga
    41341 gaaaagcgtg gagtctgcgc tggctgacta ttttgctgcc atcgccaact gctttaccag
    41401 caaagacacc ccggcaggct gcttcatgat caacaactgc accaccctct ccccagattc
    41461 aggagatata gccaatacgt tgaaatcacg ccatgcgatg caagagcgca ctttgcagca
    41521 gtttttatgt caacgacaag cgcgcgggga aatcccgacc cactgtgacg tgacacatct
    41581 ggcagaattc cttaattgta ttattcaggg gatgtcgatc agcgcacgcg aaggtgcatc
    41641 gctggaaaaa ctgatgcaga ttgccggaac gactttgcgt ttatggcccg aactggtgaa
    41701 ataagtcgca aaaaaggccc ctcagcctga gcgaatgagg ggccaaattg cagagtacag
    41761 ccttgttata ctttgttatt caaaatcagt tgcccgttac tatctagcgg gatctgcgta
    41821 cccggatcgc ggtccatgcg gattttgccc tgctgatcgc caatcttata ggtcacatca
    41881 taaccgagca ttttttctga cttgtcatac accgttttac aacgctgttg cgtagtcgtg
    41941 taagtatcgc tttcctggag agagccctgg atctggttac cggcatatcc accacccagc
    42001 gcccccacaa cagtggcgac atctttaccg cgaccaccac caaactgatg ccctatcacg
    42061 ccaccagcaa cagcgccgag caccgacccg gtaatgcgat tttcatcctg caccggtcga
    42121 cgatgggtca ctgtgacgtt gcgacactcc tgacgcggtg ttttaaccgt ttccttgatt
    42181 ggggttgcag aaacaacctg agcgtattgc gggccccgtt caaacacgtt cagactggcc
    42241 actgccgcta cgcccaacgc agctgcgaca ccaatcccga tacccgccaa cattgattta
    42301 ttcacgggaa cacctccttc tttgcagtta tgccaatcat gcaagcgaag gagaaaagca
    42361 gctatcagac agaagatgaa aagcgcggga atgactggaa atacttaagg attcggagga
    42421 gtctgaagga atgcctgatg cgacgcttaa cgcgtcttat caggcctacc gctcgtacca
    42481 tttgtccagg gcggataagg cgtttatacc acatccgcca gtgtacgtcg attaatgcaa
    42541 cttcaaacgc ggacggataa cgcggttaat actccccacc agcatcatta atccggtttt
    42601 aaagtaacca tgcagcgcaa tctgatgcat tcggtatagc gagatatata caaagcgcgc
    42661 aattcgtcct tcaatcatca ttgagccgcg cgtcaggtta cccatcaggc taccaacggt
    42721 ggagaagttc gacagcgata ccagcgaacc atgatcttta tactgataat ttttcagcgg
    42781 cttaccgttc atctgcgcca gaatgttgtt cattgcgcaa gtcgccatct ggtgtgcagc
    42841 ctgagcacgc ggcggaacaa agcccccttc cggacgcggg catgacgcgc agtcgccaat
    42901 agcgtaaatg tctggatcgc gagtggtttg cagcgtcggt tccaccacca gctggttgat
    42961 acggttagtt tcaagaccac cgatatcttt caggaagtct ggcgctttga tcccggctgc
    43021 ccataccatc agatcagcct caatatattc gccatcttta gtgtgcaggc cgccttcatc
    43081 agcactggtg accatggttt gcgtcagcac gcgaacgcca agtttcgtta gctcgttgtg
    43141 ggccgcagca gagatacgtg gcggtaatgc aggcaaaata cgttctcccg cttctaccag
    43201 cgttacgttc agggcttcgt tggtcaggcc tttgtaaccg tagctgtgca gttgcttgat
    43261 cgcgttgtgc aattcagcgg agagttctac acccgtcgcg ccgccgccga caatcgcaat
    43321 gttcactttg ccatttgcgc ccaggttggc agagtatttc aggaacagat tcagcatctc
    43381 ctggtggaag cgacgcgcct ggtgcgggtt atcgaggaaa atgcagttct ctttgacgcc
    43441 tggcgtattg aaatcgttag aggtgctacc cagcgccatg accagggtgt cataggcgat
    43501 tttacgttcc ggaaccaaca gttcaccttt ctcgtcgcgt agttctgcaa tagtgattgt
    43561 tttcgcttca cgatcaatat caatgacgga acccaactgg aactggaaac catgattgcg
    43621 cgcatgggcc agatagctca acgcatcgac gccttcatca agcgagccag tcgccacttc
    43681 gtgcagcagt ggtttccaca ggtggctgtg gttacgatcg accagcgtaa ttttggcttt
    43741 tttcttacgt cccagcttat gccccagctg tgttgccatt tccagcccac cagcaccgcc
    43801 gccgacaatc acaatctttt tcaatggcgt agtcaacgtg ccccccttaa atttattaac
    43861 caattgttaa ttaatggtta ttaacatagc tcttaattaa caacaggtta cagcaatgaa
    43921 aatgttcttc gctattgaga ataacatgaa tggtgcattg gtcataccaa aattgatgtt
    43981 aatcaagttt tgttgcttaa aagataggca actttaccgg gggagatcaa aaaatagagt
    44041 ttattgttta ttatttgagc aaattcggaa gggtaatata gggtgaagcg tatatcgagg
    44101 gcgtttggtc gtcctgataa gatgcgcagc atcgcatcag gcattgatgc tcgactgtcg
    44161 gatgcggcgt ggacgcctta tccgacttac agaattcgga gcgcatcagg taccggcaag
    44221 gttacctgat gcgacgaggg catttacccg agggttttga acgctttaat gcgctgtaaa
    44281 tgcggggaga tattcttgaa tttgtgcgtc tgctcttcgt cccagacaat ctcgtaataa
    44341 tgatgcaact cttcagatgt ccgctggctg ttaagtgctt catcattacg cgacaaaata
    44401 accagacaac gatcgcgatt cttctcgcgg aagttggtca cacacttagt ggcaatatcg
    44461 gcatactctt ccgggcgatc aatcttgcct tccatgttct cataagggaa caaattaggg
    44521 ttgaagatca cctggcggat atcgcaaaga aaaccaatcc gttccgccca gtatccgccc
    44581 aggccaacgc cgcaaattag cggacgctcg tcaacgttca gttgcaacat tttgtccact
    44641 tctttaagca gatgctgcat atcatgtttc ggatgccgcg tactgtagct tatcaagcgt
    44701 acatccgggt caataaactg caattgtaag actttctcgt ggttacccgg actgttagag
    44761 tcaaaaccgt gtaaatagat aatcatcgct tcctcacata agccagggtt agtgacctac
    44821 tttctcttcc tgccagcgtt catgtaactg attcagacgg gtgctgatcg ctttccagcg
    44881 agccgagtcc atcagttcct gtcgcgaaaa tgaaccttta tgatacaaac gtgtaacacg
    44941 ttctgccttg atcggtgaca gattatctaa cacgctgacg gcccctttac gattattgca
    45001 gaccaggatc atatcgcaac ccgcatccag tgatgcctgc ccgcgttcgg cataactgcc
    45061 cataatcgcg gcaccttcca tcgataaatc gtcagagaaa atcacgccgt caaaccccag
    45121 ttcctgacgc aaaacggttt tcagccagta gggagaaccg ctcgccggac gcggatcaac
    45181 atcactgtag atcacatgcg caggcataat ggcgtcgagt ttattttcgc ggattaagga
    45241 actgaagacc gacatatctt tagcgcgaat ctccgcttgt ggacgtggat cgcacggtgt
    45301 ttctttgtgt gagtctgccg ttactgcacc gtgtcctggg aagtgtttcc cggtcgtttt
    45361 cattccggct tcatgcatac catcaataaa ccggctggca attgccaggg ctttttgtgg
    45421 atcggcatga taagaacgct cgccaatcgc cgcgctgata tgcccgacat ccagcactgg
    45481 cgcaaagctg atatcaatat ccatagcgat catttcgctg gccatcagcc aacccgcctc
    45541 ttgcgccagt ttgccgccct cttccattcc caacagcgca gcaaaggatt gtgctgccgg
    45601 taagcgggta aaaccttcgc gaaaacgctg cacgcgtcca ccttcctgat ctaccgccac
    45661 caccagatga ttgcgcgatg ctgcgcggat ctggcgcacc agttcacgta actgggcagg
    45721 atcatgatag ttacgcgtaa agagaatcag ccctcccacc agcggatgcg ccagtatttc
    45781 acgctcttcc gcgtccagtt cgtaaccttc gacatccaac attactggac ccacactgct
    45841 ctccttattg ttttattaat agctgccgcc aggtgtcatc ggccagcctg ataaattgtt
    45901 gatcgccggt ttgtcgccag cggtactcaa accaccctcc tttgagcatc agcagccagg
    45961 gaaaccatcg cctgacctga cgccataatt gcgccggata aatcttcgcg cgagtggcat
    46021 agtcattgac caattgccgg tgctgttcag tattttccac ccacaccgcc gccagttcca
    46081 gcgcgatatc accatctcca gcatactccc agtcgatgag ttttaaccct gatgcgctat
    46141 gcactaaatt tccggcgtgg acatccatat gcaatggact taagcgtaaa ggtcgtggtt
    46201 cccgcgcttt gcgcagacgt tttaacattc gcagccaacc cacagtccgc cgcgccggat
    46261 cgctttgctg ccagtacagt tccagtaagg gcaacagcgt tattcgccag ccaaaacgtg
    46321 gttgttgatg tagataatac agcaagcctg ccagttcgtt ggtatccggc aaatacgttt
    46381 ttaccgcacc gggcagatag tcgactacca tccagtcacg gagatataaa tgcggcttcg
    46441 gtgcaatgct tgcgggtagt tgtgataaag cccgatactg gcgcaagaac gcggactgcg
    46501 gcgcatcagg atcgtgcggc tgacgcacaa caaaacgctg gccctgatgt tcaatgagaa
    46561 aactaccgcc actaagccca ctattaaacg tcgtgacggg atgaaactgc gggaaaaagc
    46621 gcgacagcaa ttcgtcgcgc gtgatgggat tattgctgcg aaacggcacc tttacctgac
    46681 cagataattt cgcccgtctg caccagcatc agctgcattt gtagggtcgg agcgttaacg
    46741 ttgccagagg cgcaggagta cagcacgtaa tgagcgccga cattgcgggc aatgcctatg
    46801 gctttactac gggtgcctaa actgtcctgc ggcgacaaac ctaactgttg cttagccatc
    46861 gacagttgct gggcggaaac cagggtaaat ttcccgttat tagccagcgc atttcgcagc
    46921 gtttcggtcg cttctgcggc attcagcgaa ccgttagtac ggttgttaac gctatcaacc
    46981 agcaggacgc tacccgcagt caccccgtca gccccaagca tcttactgac catcggctgc
    47041 attgcgccat tccagtcata atggcgaata tgcggcgcag gcggtgccgt tcgatcttcg
    47101 tgctcaattg ggcctggctg ctgcgggatc gtcggcaccg agggcactgt ggggacaggc
    47161 tgttgtggct cggctggttg ttccggcgct ggtttcactt cttctaccgg tgcaggttca
    47221 cgttgcccca cacacccggc gagaaacatc gccagcgcgg taatcaaggc gtagcgactc
    47281 atttttgtca tcaagattca cccctttaca aatatagata aagtctgact ttgtgcgccc
    47341 ccagaaaatt ggcgctgccg tacagcgtta ccgccgaatg tgcgggaatg gtaacgctgc
    47401 gtggcctttc cagaggatgc atctccagcc ctctggcgtc ataccagtaa aaacgataat
    47461 gaacggtaac gggttcttgc ctttcgttat aaatcgttga ggatgctgaa ggttgaatat
    47521 cagacgtcga aaggacgggc ttttctgcac tgatgcccgc agccaataaa gatgactcca
    47581 tcaccagcga ttgctcatca ttcaccggaa tttccggatg tgaacgacag cccaccagca
    47641 gtaacaacac cagagacacc agcccaaagc atccttttct catcgttaca gacctttatg
    47701 cgccagcatt ggtcccagcg gacggccacc caacaagtgc atatggatgt ggtaaacctc
    47761 ttgtccgcca tggcggttgg tattcatgat cagacgatag ccatcttcgg caataccttc
    47821 ttgctcagca atttttgccg ctacggtgat catgcgtccc agcgcctgct catgctcagc
    47881 tgacacgtcg ttcacagtcg gaatgagaat attcggaatg atcagaatat gcgttggcgc
    47941 ttgcggcgaa atatcgcgaa acgccgttac cagatcatcc tggtagacga tatcggaggg
    48001 gatctcacga cgaataattt tgctgaatat agtttcttct gccacgacat tttccttttt
    48061 caaatagcca tgcgttgcgc catgctacgc cgcgccacac tgcgagtata gagtatggtc
    48121 gagttaaacc tgctctttca atcttaactc acgatttctt aagcaaaaaa tcgttttgct
    48181 gccgtatata tcgccattat tcccatttct gcctgcgaat gcgctgaaaa gacattctct
    48241 gtttggttta cacgacaaat atgaatgcgt atatttctca tttgcatttg cagcaaaatt
    48301 attcgcacac taaatataac cgctcatcac cctcctccgg catgagcctg acgacaacat
    48361 aaaccaagag atttcaaatg ctttcaacac aatttaacag ggataatcaa tatcaagtca
    48421 tcaccaaacc gtcactactt gccggttgca tagcactggc actattacct tctgccgctt
    48481 ttgctgcacc agccactgaa gaaacggtga ttgttgaggg ttcagccaca gctccagatg
    48541 atggcgaaaa tgattacagc gtaacgtcta cctctgcggg taccaaaatg cagatgactc
    48601 aacatgatat tcctcagtcg gtcactattg ttagccagca gcggatggaa gatcagcagt
    48661 tacaaacgct gggcgaagtg atggaaaaca cgctggggat cagcaaaagt caggcggatt
    48721 ccgatcgtgc tctttattat tcccgcggat tccagatcga taactatatg gttgatggta
    48781 tccccaccta ttttgaatcg cgctggaatc tgggcgacgc actttctgat atggcactgt
    48841 ttgaacgcgt agaagtagtg cgtggcgcga caggactcat gaccgggacg ggtaatccat
    48901 ctgcggcaat taatatggtt cgaaaacacg cgacctgtcg tgaatttaaa ggcgatgtct
    48961 cggcggaata cggtagctgg aacaaagaac ggtatgtggc ggatttacaa agcccactca
    49021 ccgaagacgg taaaatccgc gcgcgaattg tcggcggcta ccagaataac gactcatggc
    49081 tggaccgcta caatagtgaa aagaccttct tctcgggcat tgtcgatgct gatttaggcg
    49141 atcttacgac gctttcagcc ggttacgaat atcagcgcat tgatgttaat agcccaacct
    49201 ggggcggttt accgcgctgg aatactgatg gcagcagcaa cagttacgat cgcgcacgca
    49261 gtaccgcacc tgactgggcg tacaacgata aagagatcaa caaggtcttt atgaccctga
    49321 agcagcggtt tgctgatacc tggcaagcga cactgaatgc cacccactct gaagtcgaat
    49381 ttgacagcaa aatgatgtat gtcgatgcct atgtaaacaa agcggatggt atgctggttg
    49441 ggccatacag taattatgga cctggctttg atcatgtcgg cggcaccggt tggaacagtg
    49501 gcaaacgtaa agttgatgcg ctggatttgt tcgctgacgg tagttatgaa ttgtttggtc
    49561 gtcagcacaa tctaatgttt ggtggcagtt acagcaaaca aaacaatcgt tcagttcatg
    49621 ggccaacatc ttcccggatg aaattggcag tttctacaac tttaatggca atttcccaca
    49681 agccgactgg tcatcacaga gcctggcgca ggacgatacc acacatatga aatcgttata
    49741 tgctgccact cgtgtcaccc ttgccgatcc gctgcatctg atcctcggcg cacgttatac
    49801 caactggcgg gttgatacgc tgacttacag catggagaaa aaccacacca cgccttacgc
    49861 tggtctggtg tttgacatca atgacaactg gtcgacctac gccagctata cctctatttt
    49921 ccagccgcaa aatgatcgtg acagttcagg caaatatctg gctccaatca ccggtaacaa
    49981 ctacgagctg ggtctgaaat cggactggat gaatagccgt ctgaccacca cgttagccat
    50041 cttccgtatt gagcaggata atgtcgctca gtccaccggt acacctatcc ccggcagcaa
    50101 cggcgaaacc gcctataaag cggtggatgg gacagtcagt aaaggggtgg aatttgaact
    50161 caacggcgca attaccgaca actggcagct gacatttggc gcaacgcgct atcttgcaga
    50221 ggataacgaa ggaaacgccg ttaatcctaa tctgccacgc accacggtta aaatgttcac
    50281 cagctatcgg ttgcctgtca tgccagagtt gacagtcggc ggtggtgtta actggcaaaa
    50341 tcgcgtgtat accgacaccg tgacaccgta tggcaccttc cgcgccgagc aaggtagcta
    50401 cgcgctggtg gatctcttca cccgctacca ggtgacgaaa aacttctcgt tacaggggaa
    50461 cgtcaataac ctgttcgaca aaacctacga taccaacatg gaaggttcta tcgtctacgg
    50521 cgcaccgcgt aatttcagca ttaccggcac gtatcaattc tgaataacac ctgtaaaaaa
    50581 ggcagccatc tggctgcctt agtctcccca acgtcttacg gattagtggt tacggatgta
    50641 ctcatccatc tcggttttca ggttatcgga tttagtaccg aaaatagcct gaacaccaga
    50701 accagcaacc actacgcccg ctgcgcccag tttcttcagg ccagcctgat ccactttaga
    50761 cacatcagca acgctgacgc gcagacgggt aatacatgcg tcgaggttag taatgttttc
    50821 tttaccacca aatgcagcaa ccagagccgg tgccatttcg ctggtacctg ttgcttttgc
    50881 atcttcagtc gcgtcttcac gacccggcgt tttcagatcc agtgctttaa tcagcacgcg
    50941 gaagatggtg tagtaaacaa tcgcataacc gataccgacg atcggtaaca gccacagttt
    51001 gctgctgtta ccagacagaa cgatgaagtc gatcagaccg tgcgagaacg acgtaccgtc
    51061 acgcatcccc agaagaatac agattgggaa tgccaggcct gccagaatcg cgtggatgat
    51121 gtacaggatc ggcgcaacga acatgaagaa gaactcgatc ggctcggtga taccggtcag
    51181 gaacgaggtc agcgccgcgg agatcataat accgcccact ttcgcgcggt tttctggttt
    51241 agcagagtgc cagatagcaa ttgcggcagc tggcagaccg tacattttga acaggaagcc
    51301 accagacagt ttacccgcag tcgggtcacc cgccatataa cgcggaatgt cgccgtggaa
    51361 aacctgacct gctgcgttgg tgtattcacc aatctgcatc tggaaaggta cgttccagat
    51421 gtggtgcaga ccaaacggta ccaggcaacg ttcgatgaaa ccgtaaatgc caaacgcaac
    51481 taccgggttc tggtaagcag cccactgaga gaaggtctgg attgcagaac caatcggcgg
    51541 ccaaatgaag gacagcacaa cgccagtaaa gatggcagcc aggccagaaa tgatcggcac
    51601 aaagcgttta ccggcaaaga agccaagata ctcaggcagc ttaatacggt agaaacggtt
    51661 aaacatgtac gctgcaatcg caccggagat aatccctccg agtacgccag tatccgccaa
    51721 gtgtttagag gcgatttctt cagcaggtaa atgcagtacc agtggcgcaa ccacggccat
    51781 ggttttaacc atgatgccat aggcaacaac tgcggccagc gcggatacgc catcgttatt
    51841 ggtaaagccg agggcgacac cgatcgcaaa aatcagtggc atgtttgcaa agacggaacc
    51901 gcctgcttct gccataacat gcgatacaac ggcgggcagc cagctgaaat tcgcggaacc
    51961 gacgcccagc agaatacctg cgataggtag tacggatacc ggcagcatca gcgatttacc
    52021 gaccttttgc aggttagcaa atgcattctt aaacataatt gagagtgctc ctgagtatgg
    52081 gtgctttttt tacgttctca cgcgtggcaa ggggggagag cctcgccgtg tacagggcat
    52141 ctaagcgccc tttatttatt acacagagta aaataattca gtgccaattt gtttgacggc
    52201 tatcacgttt caacttcaga tcattggaga agttgcacat tttgacaaaa catatgtcac
    52261 aatatttcaa acgcggttat tcaccgccca attttcggcg gtgaacttta ctcgttttag
    52321 ccattaatta cgagctttaa aaaaactcat tcaacggatg gattgaaggc gggaagcgtc
    52381 gatgtgaaac agacgggcga agttatcggt ggttacctgc gccagttctt caacggcaac
    52441 acctttcaac acagccatgt attctgcaac gtcacgaacc atcgcaggtt gattctcttt
    52501 tcctcgatgc ggtaccggcg caaggtaagg tgagtcagtt tccaccagta atcgatccag
    52561 ggggacataa cgcgcagcat cgcgcagttg ctccgcatta cggaaggtca caatgccgga
    52621 aaaggagatg taaaatccga gatccagtaa tttacccgcc gtttctctgt cctctgtaaa
    52681 acagtgtagt acgccaccgc aatccgtcac tttttcttcg cgcagaattg ccagcgtatc
    52741 ggcacgggcg tcacgggtat ggacgataac cggcttgttc agttcacgac caatctggat
    52801 atgatggatg aaggactctt gctgacgtac tttagtttcc ggtgtgtaat aataatccag
    52861 cccggtttca cccagcgcta caacaccctc ttctgccgcc agacggcgta aatcttctac
    52921 atcgtaggga tcattctggt tgagtggatg cacgccacaa gaaaatacga cgttgtcacg
    52981 ttcgcctacc agatcccgca tatgtaaata acccggtaat gttgtggcga ctgccagaca
    53041 aaatttcaca tcgcgtgcgg cggctttcgc cagaacgtca tccacgtcct tatgcaaaga
    53101 ttcataatcc agaccatcga gatggcagtg tgagtcgact aaaaacatga tgtctctctt
    53161 aaagatgagg aaccggtaac acaacgcccg gttgcaggta atgctcaata cgcagtaaaa
    53221 gatcggtgat gagaagctcg cggttgatgc ctgtgacaga cattaactgt tcacgaatgt
    53281 ggcaaacatc ccccagtata gcctgcaggc acgagggaga aagatggttt gccagttcgg
    53341 cgaccaggcc cggcacatca acattggtca cctgcgcagc gccatgatgg cgttttagcg
    53401 catccatcag caacgttgcc agccagtgta aacgcgccgg agcttgttca tgattaaggg
    53461 ccgccagcag cgaataccag tcgcccgatg gcacgctata tgccaacgcc tgacacaatg
    53521 tttcacgagc ctgccagtta tctccctgaa acaacgccag tgccgcgcca ggcgaaccgg
    53581 cgcttaagcg caatgcggca agtaatgcat cctgtgacat tgtcacttcg cgtgaaagcc
    53641 aagtcacggc gtactgttct ggaggcggcg caaggtaatg taaccgacaa cgactacgta
    53701 atgttgccag taaacgttca ggctcgcggg tagccaggaa aaaccaggtt tctgctggtg
    53761 gctcttcaag cgttttcagc aatgcgttag ccgcggcgtc ggtcagtaag gcagcatcgg
    53821 ttacccagac aactttcgca ccacctaggc gtgcgtgctc attcagcttt tcggtgacct
    53881 cacgtaccgc atcaatgccc agcgtatttt tccctttttc gggagccagg gtgtagtaat
    53941 cgggatgcgt gccagcctgc atcaactgac atccacgaca gtgaccgcaa cttttgtggc
    54001 cctgcggttg ttggcagagt aaataacggc tcagggcgta gattaaagca tcatcgccca
    54061 tgcccggtaa cgcctgaatg agtagcgcat ggtgacctct tccggcctga tagctggcta
    54121 ccagtttttc gaaatcaggt cgtaaccatg gataccatct catgcgtcca actccttcac
    54181 ccagtgggtc acggtagtgc ggattgcatc catcacggcc tccagcggct gggtggcatc
    54241 aatggtatga atacttttat cttgtgcagc cagttccaga tagcgggcgc gggtgcgatt
    54301 aaagaaatcg aaagactctt gctcaatacg atccagctcg ccgcgcgcac gcgcgcgttt
    54361 taagccaact tccggggtaa catcgagata gagcgttaag tctgggcgaa aatccccgag
    54421 aacagcatca cgcagtgttg ccagcatatg ttggtcaata ccacgtcctc cgccctgata
    54481 cgcctgagtg gaaagatcgt ggcgatcgcc aatcacccag gtgccgttag ccagcgcagg
    54541 tttgatgacc gtttctacca gttgaacgcg cgcggcataa aacatcagaa cttcggcttt
    54601 atcggtaatg acttcatcgc ctaccgattt gatatccagc accaggcttc ttaacttttc
    54661 ggcaagttgc gtaccgccag gttcccgagt gaaaaccatg tcgcggatac ccagttgctc
    54721 gagcgtctca accaccacat tacgcgcggt agttttgcct gcgccttcca gcccctcaat
    54781 gacgatatac ttactgcgca tttttttcct taagcacttt cagataatcc tgcacagact
    54841 tgttatgact ggcaagattg gtattaaacg tgtgaccacc tttaccatcg gccacaaaat
    54901 agagatacgg cgtttttgcc ggatgcgcag cagccttcag cgaatccgcc cccggcgtag
    54961 ctatcgcacc cggcggcaaa ccggtaatgg tataggtgtt atacgctgtc ggcgtttcca
    55021 ggtctgcacg agaaagtttg ccattataac gctctcccat cccgtaaatc acggttgggt
    55081 cggtctgcag gcgcatacca atgcgtaaac ggttgataaa taccgaggca acctgatcgc
    55141 gttcactggc aacggcggtt tctttttcga taattgatgc catcgtcacc agctgatttt
    55201 tatctttata aggcagaccg tccgcacgcc cttcccaggc gctatcgacc gctttcacca
    55261 ttttcttgtg aactcgcttg agtaacgcga catcggtggt attggcggta tacatcaagg
    55321 tgtctggcca gaaccaacct tcaatccact ccgggttttc cagttcaagt gcctgcgcta
    55381 cggtggcgta cttatcgtca ctcagcgtgt gcttgatata cggagcctca cgcaattgct
    55441 tgaggtagtc gctcagacgc atcccttcta ccagtcgcag cgggaactgt gcttctttac
    55501 cgctttccaa caatttcagc atctcgcgca cggtcatctg cggtgtaaag cggtaagtcc
    55561 cggctttaaa gtgagaaaga tccggttcga tacgcagcag ccattgaaaa acccgtgggc
    55621 gattgatgat cttatcagca taaagctgtt caccgagcgc cagacgtccg gtccctggct
    55681 tcagggtaaa tatcgtctct tctttgataa gcaatttgct gtcggcaaga tggcgaacct
    55741 tccagacgcc cacaccagcg gcgataccca gtaccaccag caataacaag ataatcaata
    55801 acactttttt catgactaat tcgggcgctc acaaagtggg gctaaatatt catataacgt
    55861 tgctgacgaa aaggtgacat cgccacaggc acgtacgggc atcactggca ttaacgcatt
    55921 acaaataacc atctcatctg cctgcaacgc ctcctccagc gaggcttgca cttcgacaag
    55981 ctgataagag gattgtgcca gtaaacggat acagaattgt cgcataatgc cgttaacacc
    56041 tgcctgatcc agtcgcggcg tataaactac attgcccttc cgccagaaca aattagccgc
    56101 acagcattcc gtaacccacc cttcgctgtc aaggaccagc gcctcatcag cgtttgtctg
    56161 ctcaagatga gagcgaatca atacttgctc aagccgatta agatgtttaa tacctgcaag
    56221 atgaggattg cgccccagcc gcaccgggct tagcgccaac gtcatccctt cgttacgcaa
    56281 acggtcgtaa tgtgcaggat aagccgtaac ggagagaatc cgcgttgctg gtccgctgtt
    56341 caatgtgctg taccctcgcc cgccactacc gcgactgatc acgactttca gcacaccatt
    56401 ttgctgttct gctgccagcg ttttcatctc ttgttcaagc tgaggccaga agtcacagga
    56461 aatcatcaac cgctgacaag catcctgtag tcgcaggata tgcgccgata acaaactgac
    56521 tttaccgtcg ataactctgg cggtggtaaa acaaccatca ccaaactgcg ttgcccgatc
    56581 gcttactgcc agcgattcct gcttataacc gttaattaag aacataccgg ctccttatca
    56641 gagaattgtt agtgtggcag catgttcact acggaacaag tcggaataaa agctaagaaa
    56701 aaaggcccgc aagcggacct tttatatggg tggaaaatga caacttagat ctttttaaag
    56761 atcaaagaac cattagtgcc accgaagccg aaggagttac acagcgtgta ttccattccg
    56821 ctgacctgac gtgcttcatg cggtacgaaa tccagatcgc aaccttcatc cgggttatcc
    56881 aggttgatgg ttggcggaac agcctgatcg cgcagcgcca ggatggagta gatagattct
    56941 actgcacccg ccgcacctaa caggtgaccg gtcatagatt tcgtggagct taccaacaca
    57001 cggcttgcag cttcaccgaa gatggttttc accgcctgcg cttcagcttt atcgccagcc
    57061 ggcgtagaag taccgtgcgc gttaacgtag ccaatctgac tcgcttcaat gcctgcatca
    57121 cgcagagcat ttgccattgc cagagctgcg cctgcgccat tttctggcgg tgacgtcata
    57181 tgataagcat cgctgctcat accaaagccg acgagttcag cgtaaatttt cgcaccgcgt
    57241 tttttcgcgt gttcgtactc ttcaagtacc agcataccgg caccatcgcc cagtacgaag
    57301 ccatcacgct ctttatccca cgggcggctc gccgcttgcg ggttatcatt gcgggtagat
    57361 aatgcacgtg ccgcgccaaa accaccaacg cccagcggcg tactggcttt ctctgcgcca
    57421 cctgcaacca tcacgtcagc atcgccatac gcgataatac gcgcagcatg gccaatgttg
    57481 tgcacgccgg aagtacaggc ggtcgcgata gagatgcttg ggccacgcag gccatacatg
    57541 atagtcagat gacctgccac catgttcaca atcgttgacg gaacgaagaa tgggctgatc
    57601 ttacgtggac caccgttcat cagagatgtg tggttttctt cgatcagtcc gaggccgcca
    57661 atcccggagc caattgcggc accaatgcgg gttgcgttct cttccgttat ttcaaggcca
    57721 gaatcctgca tggcctgaac gccagcgaca attccatatt gaatgaaggc atccatcttg
    57781 cgctgttctt tgcgcgagat aatgtcctca cagttaaaat cctttactaa gccagcaaat
    57841 ttcgttgcat aggcgctagt atcgaaatgg tcgattaggc tgatgccact ctgaccggca
    57901 agcagagctt tccaggtaga ctctacggta ttgccgacag gagacaacat gcccagtccg
    57961 gtcacaacta cacgacgctt agacacgttt gtcctccaag gagggaaaaa tgattctagt
    58021 gggacaaaaa gataaaactc aggcggtcga acgaccgcct ggagatgttc acttacgcct
    58081 ggtggccgtt gatgtaatca atggcagcct gaacggtggt gattttctca gcttcttcgt
    58141 ccggaatctc agtatcaaac tcttcttcca gagccattac cagctcaacg gtgtcaagag
    58201 aatccgcgcc caggtcttca acgaaagaag cattgttggt aacttcttcc tgcttaacgc
    58261 ccagctgttc gccgataatt ttcttaacgc gttcttcgat agtgctcata ctcttaaatt
    58321 tcctatcaaa actcgctttc gcgatggttt tcgtagtgta taaaatgttg aaaaatttgc
    58381 aactaaatcc cggcaggtct taccacgatt ttacgttatt ttgaggcctt accccccaat
    58441 aacgcaaatc attttgcact aattattact cataaccacg cagactgcgc gcaacatgag
    58501 cattttgtgc aaatcgcggt cagaccatgt acatcccgcc gttcacatgc aaagtttcac
    58561 ccgtgatgta agctgcttcg tcggatgcca ggaatgcaac cgcgttggcg atttcctgtg
    58621 cgccgccgag gcgacccgca ggaacctgcg ccaggatacc cgcacgctgg tcatcgctca
    58681 gcgcacgtgt catgtccgtt tcaataaagc ccggagcaac aacgtttaca gtaataccgc
    58741 gtgacgcaac ttcgcgcgcc agtgatttac tgaagccgat caagcccgct ttcgccgcag
    58801 cgtagttggc ctgaccgcca tttcccatgg taccaaccac agaaccgata gtgataatac
    58861 gaccatgacg ctttttcatc atagcgcgca ttaccgcttt tgacagacgg aaaacagatg
    58921 aaaggttagt ttcgataata tcgttccact cttcatcttt cattcgcatt aacaggttat
    58981 cacgagtgat accggcatta ttgaccagga tatccacttc accaaattct gcgcgaattt
    59041 tttccagaac agattcgata gatgccgggt cggtcacatt caacatcaga cctttgccgt
    59101 tggcacctaa ataatcactg atcgcctgag cgccattttc actggtcgca gtgccaataa
    59161 ctttcgcgcc acgggctgcg agcgtttcag caattgcgcg gccaattccg cggcttgcac
    59221 cggttaccag tgcgattttt ccttcaaaat tcatgatttt cctcttttaa agctcgagcg
    59281 ccgctgccat cgctgaaggt tcgttcagcg ccgaggcggt cagggtgtcg acaatgcgtt
    59341 tcgtcaggcc agtaagcacc ttgcccgggc cgacttcata gagatgttct acgccttgcg
    59401 ctgccatgta ctcaacagac ttcgtccact gaaccgggtt atacaactga cgtaccagtg
    59461 cgtcacggat ggcatcacca ttggtttcgc atttcacatc aacgttattc acaacagaaa
    59521 ctgttggtgc gttaaaggtg attttcgcta attctactgc cagtttgtcg gctgctggtt
    59581 tcatcagcgc acagtgagac ggtacgctca ctggtaacgg cagcgcgcgt tttgcgccag
    59641 cagctttaca ggcagcgcca gcacgctcaa ccgcttcttt atgaccggca ataaccacct
    59701 gtcccggaga gttaaagttt accggagaaa cgacctgacc ttctgcagct tcttcacacg
    59761 ctttcgcaat aggcgcatca tccagaccga tgattgccgc catagcgccc gtgccttccg
    59821 gtacggcttc ttgcatgaac ttaccgcgca tctcaaccag acgcaccgca tcagcgaaat
    59881 caatcacgcc agcgcaaacc agcgcggagt attcccccag gctgtgaccg gccatcattg
    59941 ccggtgcttt accgccctgc tgctgccata cgcgatacag cgcaacagat gcagtcaaca
    60001 gcgctggctg ggtttgccag gttttattca gttcttcagc tgacccctgc tgggtcagcg
    60061 cccacaggtc gtagcccagc gccgcagaag cttcagcaaa cgtttcttcg acaattggat
    60121 agctcgccgc catatcagcc agcattccaa cggtttgaga accctgtcca gggaacacaa
    60181 atgcaaattg cgtcatgttt taatccttat cctagaaacg aaccagcgca gagccccaag
    60241 tgaatccacc gccaaaggct tcaagcagaa ccaactgccc cggcttaatg cgcccgtcgc
    60301 gtacagcttc atccagcgcg cacgggacag aggccgcaga ggtattaccg tggcgatcca
    60361 gcgtcaccac gacattgtcc atcgacatac cgagtttttt cgccgttgca ctgataatac
    60421 gcaggttagc ctgatgcgga accagccagt ccagttgaga acggtcaaga ttattcgccg
    60481 ccagcgtctc atcaacgatg tgcgccagtt ccgttaccgc aaccttgaag acttcgttgc
    60541 ccgccatcgt cagatgaatt gaattctctg gattcacgcg gtcggcgttt ggcagcgtca
    60601 gcaattcacc ataactaccg tcggcatgca gatgggtgga aatgattccc ggctcttcag
    60661 aggcagccag caccgcagcg cccgcgccat cgccaaaaat aataatagtc ccacgatctg
    60721 ttggatcgca ggtgcgcgcc agtacatcgg aaccgacgac cagagcatac ttcaccgccc
    60781 cagatttcac gtattgatcg gctacgctta atgcataggt gaaacctgca caggctgctg
    60841 caacgtcaaa tgccgggcaa cctttaatgt ccaacatgct ttgaatctga caagctgcgc
    60901 tcgggaaagc gtgcgtagca gaagtcgttg ccacaacgat caggccaatc tggtctttct
    60961 caatgcccgc catctcaatt gcgcgtgtcg ccgcttcaaa gcccatggtt gaaacggttt
    61021 cgtttggcgc ggcaatgtgg cgttcgcgga taccggtacg agtgacaatc cactcgtcag
    61081 aggtgtccac cattttttcc aaatcggcgt ttgtccgcac ttgttcgggc agatagctgc
    61141 cagtaccaat aatcttcgta tacatgtacg ctcagtcact tttcggttat ataccgtcac
    61201 ttgcaaactg cgagttcgct ggcagcgtcc tgctaccgca gagttccgct tttgccaccg
    61261 tccagcagct caaaaccagc tgggtataca gattccaggc gagcggcaat tcgctgagga
    61321 acttgtcgct gcaccgcctg cactgcctgt tcaatcgcga ccgcaaaagc tcgctgattg
    61381 gctgcaccat gactttttat caccgtgccg cgcaatccta acagacaggc gccgttatac
    61441 tggtcggggt tgaggtgact gaatcgcctc gtcaggctct tttgtagcca acgctttaat
    61501 aacagtagcc accacgaccg ttttttccct tcaccctgag atttcagcag agaaaggaac
    61561 atcctgacaa caccttccat cgtctttaac gtgacatttc ctgtaaagcc gtcacaaacc
    61621 agcacatctg tcttgccagt taacaactca ttggcttcaa gatagccgat ataattgata
    61681 gaagggattg ttttaagcac cgctgaggca tcccgaatac tgtcaagacc ctttacttct
    61741 tcttcaccaa tattgagcaa cgccacgcga ggattgggaa tttccaccac ctcttcagcc
    61801 agaactgagc ccataatggc aaattgcacc aacattgtgc tgtcacaatc gacgttggcc
    61861 cctaaatcga ggaccaccgt tttgcccttt tgctgatgtg gtaataccgt caccagcgcc
    61921 ggacgctcaa tcccctccag gggcttgagt aataattttg ccagccccat cagtgccccg
    61981 gtattaccgg cactgacaca ggcttgcgct cgaccttctt tcaccagctc cagggccata
    62041 cgcattgaac tcccacggct ggcgcggata gcttgcgaag gccgggcatc actggcgata
    62101 actgactgcg caggaataat ctgcagacgc gaacgttgtt caaagtcagc tttagcaagt
    62161 aatggcgtga tggcgtcggg attgccgact aaaagaagag tgagttgcga attagaattc
    62221 agtgcctgca atgctgcagg cactgtcacg gaagggccaa aatcccctcc catgacatct
    62281 aacgccaggg ttagacgtgt caaggtatcg tcgccgcccg gtttggtctt tccccagttg
    62341 cctggggaaa atcctcacta agcttcatca cgcagacgcg tgattactta gcgatgacct
    62401 tgcggccgcg gtagtaaccg tcggcagtga tgtggtgacg caggtgtttt tcaccagaag
    62461 ttttgtctac agacaggctg gtgactgcgg tcagcgcgtc atgggaacga cgcatgccac
    62521 gtttggaacg ggttggttta ttctgttgta cggccatgga ccttactcct caattacggt
    62581 ttatccccga tccaacgggg agcaccaatt acttacgctt taagctggct aatacggcaa
    62641 atgggtttgg cttttgcact tcttcaggca gttcaccaaa gaccatgtcc gcttcggaca
    62701 cttcacagtg ttcagaatca tgcaccggaa ctaccggcaa ggcgaggatg atttcatctt
    62761 caaccattgc aagcagatcg atttcaccga attcgttaac ctcaatcggt tcatacgctt
    62821 ccggcagtgc ttcagcctgt tcgtctgaac gcacaggact aaaacaatac gttgtgtaga
    62881 cctgatgagt aaacggcttc ccgcaacgct gacactcgag cgttaccgtc accttcgcat
    62941 cgccgtttaa cactgcgaga cgttggttat cgatagcgaa cgacatggag cattccacat
    63001 cactgtccac actgactacg gattcggcga cgcgctcaac ctgatcaggg gtatagatac
    63061 cctggtaatc aaggcgtttt tgagccgtac gaaccggatc gagagtcagg ggtaatttta
    63121 ccttttgcat agggcgcgca tattaacttt gtaacgtcat agagtcaaag aaaaaggcag
    63181 cctgtggttg ccttttgcca ataattcgca cacattgcgg gttactgctt tattttcgtt
    63241 cagtgctgat aacgaacatc aacctgcaaa acgaagcgca tagtttaaaa tggctatcat
    63301 caatacgcta tatctgatgg aaaaaaatat gcctaaactt attttagcct ccacctcgcc
    63361 atggcgtcgc gctcttctgg aaaaactgca aatctctttt gaatgtgcag cacctgaggt
    63421 cgacgaaacc ccacgcagcg acgaatcacc gcgacagttg gtgcttcgac tggcacaaga
    63481 aaaatcgcaa tctctggcgt cacgttatcc ggatcattta attattggtt cagaccaggt
    63541 atgtgttctt gatggcgaaa tcaccggtaa accgttaacg gaagaaaatg cccgtctgca
    63601 attacgcaaa gccagcggca atatcgtcac cttctatact ggactggcgt tgtttaattc
    63661 ggcgaatggg catctacaaa cagaagtgga gccttttgac gtccatttcc gtcacctaag
    63721 cgaggcggag attgataatt acgtgcgtaa ggagcatccc ctgcactgcg cgggtagctt
    63781 taagagtgaa ggatttggca ttacgctgtt tgagcgttta gagggacgtg accctaacac
    63841 gctggttggt ttaccgctaa tcgcgctgtg tcagatgtta cgtcgggaag ggaaaaaccc
    63901 gctgatggga tagttgtttg cgccggatgc ggttacgtcg catccggcaa tcggtgcagg
    63961 cggcctgatg cggcgtaaac gccttatcag gccaaccgcc cattatcagg cttatatgct
    64021 tgtattagcg cgcgttacgc agcttttgca aacaacgctt caaaccttca tccatcggcg
    64081 cttcgatacg catcacctca ccggtccccg gatgggtaaa cttcaacgct gcggcgtgca
    64141 ggaacagacg atttaatccc gtgcctgctt cagtgagctg tctgtcaaat tcacggtcac
    64201 cgtagcgatc gtcaaaggca atcggatgac ccgcatactg tgtatgcaca cggatctgat
    64261 gagtacgtcc ggttaccggg ctacaacgca ccagggtggc aaatgcatag cgttcttcca
    64321 ctttaaagcg tgtttccgac ggtttgcctt cctgactgac acgcacgata cgttcgccgc
    64381 tttgcagaat atttttcaat aacggcgctt gaacgctctt cacatgcgac tgccactgac
    64441 cgcgcaccag cgccaggtaa tctttttgca ttcctttttc acgtaattgc tcatgcagag
    64501 aacgcaacgc cgagcgtttt ttcgctacca gcaacacacc tgaagtgtcc cggtcgagac
    64561 gatgaaccag ttcaaggaac cgcgcttccg ggcgcaacgc ccgcaaacct tcgataacgc
    64621 cgaagcttaa accactgccg ccatgtaccg ccgtaccgga aggtttattc agcaccagga
    64681 tatgatcatc ttcatataag atgacgtccg ccagcgccgc caccttttgc agatgtggcg
    64741 aaaccgcctc ttcttcccgc tcagcaacgc gaaccggtgg gatacgcacc tcatcacccg
    64801 cttcgagttt atattcaggc ttaatacgtt ttttgttcac ccgcacttca cctttacgca
    64861 aaatacggta aatcatactt tttggtacgc ctttcaattg ggtacgcaaa aagttatcga
    64921 tacgttgccc cgcttcgtca gcggtgatag caacaatttt tacggatgga gtctctgttt
    64981 tcatggtggg cgattctaat tagccaacag gattcgcgcc actcattttt ctatgcttat
    65041 ttttagtttt gcaccttatt acttcactac gtgatcactt tattgatggt tattaaacca
    65101 atcaccagca agaagtgaaa aaactgtgag taagcgggtg ataaatggta aaagtcatct
    65161 tgctataaca aggcttgcag tggaataatg aggccgtttc cgtgtccatc cttgttaaaa
    65221 caagaaattt tacggaataa cccattttgc ccgaccgatc atccacgcag caatggcgta
    65281 agacgtattg atctttcagg cagttagcgg gctgcgggtt gcagtcctta ccggtagatg
    65341 gaaatatttc tggagagtaa tacccagtct gtttctttga taattgcgct gtttttccgc
    65401 atgaaaaacg ggcaaccgac actctgcgcc tctttgagct gacgataacc gtgaggttgg
    65461 cgacgcgact agacacgagg ccatcggttc acacccggaa aggcgttact ttgcccgcag
    65521 cttagtcgtc aatgtaagaa taatgagtaa gttacgatga aaagaatgtt aatcaacgca
    65581 actcagcagg aagagttgcg cgttgccctt gtagatgggc agcgtctgta tgacctggat
    65641 atcgaaagtc cagggcacga gcagaaaaag gcaaacatct acaaaggtaa aatcacccgc
    65701 attgaaccga gtctggaagc tgcttttgtt gattacggcg ctgaacgtca cggtttcctc
    65761 ccactaaaag aaattgcccg cgaatatttc cctgctaact acagtgctca tggtcgtccc
    65821 aacattaaag atgtgttgcg tgaaggtcag gaagtcattg ttcagatcga taaagaagag
    65881 cgcggcaaca aaggcgcggc attaaccacc tttatcagtc tggcgggtag ctatctggtt
    65941 ctgatgccaa ataacccgcg cgcaggtggc atttctcgcc gtatcgaagg cgatgaccgt
    66001 accgaattaa aagaagcact ggcaagcctt gaactgccgg aaggcatggg gcttatcgtg
    66061 cgcaccgctg gcgtaggcaa atctgctgag gcgctgcaat gggatttaag cttccgcctg
    66121 aaacactggg aagccatcaa aaaagccgct gaaagccgcc cggctccgtt cctgattcat
    66181 caggagagca acgtaatcgt tcgcgccttc cgcgattatt tacgtcagga tatcggcgaa
    66241 atccttatcg ataacccgaa agtcctcgaa ctggcacgtc agcatatcgc tgcattaggt
    66301 cgcccggatt tcagcagcaa aatcaaactg tacaccggcg agatcccgct gttcagccac
    66361 taccagatcg agtcgcagat cgagtccgcc ttccagcgtg aagtccgtct gccatccggt
    66421 ggttccattg ttatcgacag caccgaagcg ttaacagcca tcgacatcaa ctccgcacgc
    66481 gcgacccgcg gcggcgatat cgaagaaact gcgtttaaca ccaacctcga agccgccgat
    66541 gagattgctc gtcagctgcg cctgcgtgac ctcggcggcc tgattgttat cgacttcatc
    66601 gacatgacgc cagtacgcca ccagcgtgcg gtagaaaacc gtctgcgtga agcggtgcgt
    66661 caggaccgtg cgcgtattca aatcagccat atttctcgct ttggcctgct ggaaatgtcc
    66721 cgccagcgcc tgagcccatc actgggtgaa tccagccatc acgtctgccc gcgctgctcc
    66781 ggtaccggta ccgtgcgtga caacgaatcg ctgtcgctct ctattctgcg tttgatcgaa
    66841 gaagaagcgc tgaaagagaa cacccaggaa gttcacgcca ttgttcctgt gccaatcgct
    66901 tcttatctgc tgaatgaaaa acgttctgcg gtgaatgcga ttgaaacgcg tcaggacggt
    66961 gttcgctgcg tgattgtgcc aaacgatcag atggaaaccc cgcactacca cgtgttgcgc
    67021 gtgcgcaaag gggaagaaac gccgacctta agctacatgc tgccgaagct gcatgaagaa
    67081 gcgatggcgc tgccgtctga agaagagttc gctgaacgta agcgtcctga acaacctgca
    67141 ctggcaacct ttgccatgcc ggatgtaccg ccagcgccaa ccccagctga acctgccgcg
    67201 actgtcgtag ccccagcacc gaaagctgca acggcaacac cagcagctcc tgcacaacct
    67261 gggctgttaa gccgcttctt cggcgcactg aaagcgctgt tcagcggtgg tgaagaagcc
    67321 aaaccgaccg agcaaccaac accgaaagca gaagcgaaac cggaacgtca acaggatcgt
    67381 cgcaaacctc gtcagagcaa ccgccgtgac cgtaatgagc gccgcgacac ccgtagtgaa
    67441 cgtactgaag gcagcgataa tcgcgaagaa aaccgtcgta atcgtcgcca ggcacagcag
    67501 caaactgccg agacgcgtga gagccgtcag caggctgagg taacggaaaa agcacgtacc
    67561 accgacgagc agcaagtgcc gcgtcgtgaa cgtagccgcc gccgtaacga tgataaacgt
    67621 caggcgcaac aagaagcgaa ggcgctgaat gttgaagagc aatctgttca ggaaaccgaa
    67681 caggaagaac gtgtacgtcc ggttcagccg cgtcgtaaac agcgtcagct caatcagaaa
    67741 gtgcgttacg agcaaagcgt agccgaagaa gcggtagtcg caccggtggt tgaagaaact
    67801 gccgctgccg aaccaattgt tcaggaagct ccagctccac gcacagaact ggtgaaagtc
    67861 ccgctgccag tcgtagcgca aactgcacca gaacagcaag aagagaacaa tgctgataac
    67921 cgtgacaacg gtggcatgcc gcgtcgttct cgccgctcgc ctcgtcacct gcgcgtgagt
    67981 ggtcagcgtc gtcgtcgcta tcgtgacgag cgttatccaa cccagtcgcc aatgccgttg
    68041 accgtagcgt gcgcgtctcc ggaactggcc tctggcaaag tctggatccg ctatccaatt
    68101 gtacgtccgc aagatgtaca agttgaagag cagcgcgaac aggaagaagt acaagtgcag
    68161 ccgatggtga ctgaggtccc tgtcgccgcc gctgtcgaac ctgttgttag cgcgccagtt
    68221 gttgaagaaa tggctgaagt cgtagaagcc cccgtaccgg ttgccgaacc gcaaccggaa
    68281 gtggttgaaa cgacgcatcc tgaagtgatt gccgccgcgg taactgaaca gccgcaggtg
    68341 attaccgagt ctgatgttgc cgtagcccag gaagtcgcag aacacgcaga accgatggtt
    68401 gaaccgcagg aagagacggc agatgcagat attgaagaag ttgccgaaac tgctgaggtt
    68461 gtggttgctg aacctgaagt tgttgctcaa cctgccgcgc cagtcgtcgc tgaagtcgca
    68521 gcagaagttg aaacggtggc cgcggttgaa ccagagatca ccgttgagca taaccacgct
    68581 accgcgccaa tgacgcgcgc tccggcaccg gaatatgttc cggaggcacc gcgtcacagt
    68641 gactggcagc gccctacttt tgccttcgaa ggtaaaggtg ccgcaggtgg tcatacggca
    68701 acgcatcatg cctctgccgc tcctgcgcgt ccgcaacctg ttgagtaata attagctcaa
    68761 agtaatcaag ccctggtaac tgccagggct tttttatttc atgtttgaat caacatatta
    68821 aaattcatca aatggcatcc ttgctaacca acaatgcaaa ataggcaaaa aaaatgcccg
    68881 ccccggcgat cccagagcgg gcagacaaaa catacccagt ttcatgatat gttttaaagc
    68941 gagcttattt gttgagctgg aagagcgaca atccctgcat atcggtaaat gctttatacg
    69001 atgcctgcaa tgccgtttgc tgcatgatgt aagatgaaat agttgcattc cagtccacat
    69061 caaccagatc gctcatctgc tgcgtttgcc ctaaagcgcg atcgctacct aaggaatcca
    69121 gcgactccag tttgttcagc tgcgtgccta attccgcgcg aacggtaagg acattattca
    69181 gcgagttttt cagtccacgg ttggttttat ccaatgccgc agcggcgatt tctttatccg
    69241 cttcgctatc cgcgaccggc gttttcaggg ctgcgatggc gctatccagc atggcaaaaa
    69301 gattggtttc agaagcgcta ccgtctggtt ccgctaccgc gttactggta atactgtcga
    69361 aaattttgtc gcccgtatgt cctatcacca tcgaacgcga agcatcgacc tgttgcttaa
    69421 tactttctgt tccgccaaca taatcaccgt tagcctcact aaacggcgca gtctctgttt
    69481 tatatgtagc gcccattacc gtcagtggtg ttcgccagat tcagcaactg gtcacgaagc
    69541 ccctgaatat ccgtagccag cgaggcccgg tcatcgtcac tcaaggtgcc attgctggcg
    69601 tagacaattt tttcctgagc attctggata gcagtggtga cctggctaag tacactctct
    69661 tccagtgaca ctttttgagt ggcgaaagta cgtgccagcg tgtactggct gttttgcgcc
    69721 tgtgcctggg agagaacaac agcttgtgat gcagcaatgg gatcgtctga agggttaacg
    69781 actcgcttac ccgtcgacat ctgctcgccg tacttcatcc attctgcctg agaattggtg
    69841 atgccgcgca tgttttgctg gtacatcatc tgggtactga aacgcatttt atctcccctt
    69901 agcgaatgtt aatcagcgca tcaaaaatcg cgtttgccgt ctgcagaacc tgcgcattcg
    69961 ccaggtaata ctgctgaaaa cgttgcagat ttccgtactc ctcatcgaga ttgacaccgg
    70021 aaatcgactg ctgctgattg gaaagctgcg tcaccacatt accttgcgtg gtgctactgg
    70081 ttttcaacgt cgcggtttta ttaccgatat cactcactaa cgaggcataa gcgtcgttaa
    70141 aggatttcgc accgcccacc gttttactgt tgctttgcag atccagcagg gcctgaccgt
    70201 tgcggttgtc gctatcaccc gcatcttctt cgctcgccat cgcgattttc gcttcgtcgg
    70261 tgattaatac atccatgttg acgatggcgt cacttactgg tttcagcgtg aagctgtcgt
    70321 taacggcagg cgttcctgta aacgtcaact ccagaccatc aaatgccact ttaccgttgg
    70381 catctggtgt cgccgtaaaa gtggtattgc tggcaaggcg ggtgacctgc cactgattat
    70441 tatcgaacga gattttgtaa tctgtcgcca gtaccacgga ggcatcagtt accgtggcac
    70501 cgatcgcaac gtcacctttg tttttcgtgt tttgcagaac cgcgggctta ccgatagtaa
    70561 agaaatcttc accagcatcg ccgttagcat caaatccggc tttgtgttgg ctgttgaaag
    70621 cctcggcaaa tgccagcgcc agttgtccaa gcgtattacg cgtctggtcc agatcctgag
    70681 aacggaatgt cagaatgccg cccagcgacc cggtattcag taatttctcc gggatctcaa
    70741 tattgcctgc cgtcccatca acgtaagcga cagtcgtacg agaagggtcg gcgctggaag
    70801 gaactgccgc cagttgccgc gccgtacttc cctgaaccag tgagtaacca ttggcatcgt
    70861 gatgttataa gtaccgccat cctgaacgct gacttctacg ccaacaatct ggtttaattc
    70921 gctcaccagt tgatcgcgtt gatccagcag attgttaggt gacgcccctg cccccacgcc
    70981 tgtcaggcgc gagatttgat cgttcaggct ggcaatttgt ttagcgtaat tgttgatctg
    71041 atcaacgctg gcacctatcg cgatattgac ctgtttgtcc tggtcgcgca gatattgatc
    71101 ggtggtttta aactgattca ccaacccttc tgatttccca atcagcgcct ggcgcgccgc
    71161 cgggtcttcc gcgttactca ccagcgtttg caggctggtg aagaaatcct gcatctgtgt
    71221 tgccagcgaa gaggtactgg tggagagcat attgtcgatt ttcgacatct gctcatagcg
    71281 ggcggtcagg ccgctacttt gcgtctgcgc agcacgtaac tggttggtaa taaacgtatc
    71341 atactcacgc tgcacaccag aaacgtagac gccattgcca acccagccgc cagcgcccaa
    71401 cgtgctattg gcctgcgcca taatagtggt ttggcgggta tatccggcaa cgttatagct
    71461 ggagatatta ttacttgccg tatttaacgc cgcctgggcc gcgttcagtc cgctcatggc
    71521 gttattgatc aagctggaca tggaggttcc ttataagcct tcaattacag agtattatcg
    71581 gcagcgaccc gccggacttg agttattcag aacagattat caatgttcat gctgtaggtt
    71641 ttgctcacct tgtcgcttat cgatttcatc tgctggatca tgttggtgag tttacgggca
    71701 tagtgaggat cggtagcata gcccgcgtcc tgtaggacct gcgccccctg ttccgcactc
    71761 gcggcggtcg tcacggcggc gtagcgcggg ttacgtgtta acagcccaac gtaatccgac
    71821 aatgcttcca gatacgagct gtagacccga aacttcgctt ttactttctt cgcttcgccg
    71881 ttttcatatt cagtcgtggt gatttcagtg acttgccctt tccagttgcc agaggctttg
    71941 acaccaaaca ggttatagct tggctcgccg ttttcgcggc ggatttgccg ttgcccccaa
    72001 ccagattcaa gcgcagcctg agcgaggatc aaatgatgtg gcacaccgct ttgctggctt
    72061 gccagttggg cgggcagcga gagttgcgcg aggaatgctc tactgtcacc cggcagcgaa
    72121 tcatcgtagt tacgtggtac ggccttttgc accagctgcg aaagcgtctg gttttgataa
    72181 cgcaccacgg tttcgagcgg gaatttcatc ggtgctgctg gcgtggactc ctctggcaat
    72241 ggttgttctg gcgtcatctg tttaaccatc atctctgcaa gccccagacc tttgcccgcc
    72301 gtcatctgtt gggcaatttg ctggtcatac atactggtat acagacgagt gtgctcgctg
    72361 ctgaacaggc catcttttgg taaagcgtcg cgcatgcttt tcaacatcat ctgcacgaac
    72421 atcccttcca cctgacgggc caccggacgg atatttgccg ccggatcttc gctcgctttc
    72481 gcctttagtt cgttgagtga ttgcgcatcc caggccgcac ttgccagtag tttgctgtcg
    72541 ctgatcatca gatgatttcc agttttgccc gcagacatcc cgcactttgc attgattgca
    72601 aaatagacat cagatccatc ggcgtagcgc ccagcgcatt gagcgcacgc accacgttat
    72661 tgaggctggc gctggaacgt acgctttgca gcgaaccgcc gctctggcgt aaatcgatct
    72721 gcgtttgtgg agtaacaaca gtctgtccac caccaaacgg tgtatctggc tggctgacat
    72781 tggcctgacg attaactgtt actgagagat ttccctgcgc taccgcgcag ctgtcgaggg
    72841 tcacttcgcg attcatcacc accgaaccgg tgcgcgagtt aatcactact ttagcgtcct
    72901 gcggggtgac attaacctgc atattctgga tatcggcaag gaaacggacc tgggaactgt
    72961 tgccacttgg tacgcgcacc tgaatagtcc gcgcatctaa cgcggtggcg ctgccatatc
    73021 cacgcacgcg gttgatggtg tcagcgattt gctgcgccat gctgaaatct tcgtcgttaa
    73081 gttgcaaatt aagggtattc ccgacgccaa actggctggg caattcacgt tcaataaccg
    73141 caccattggt gatccgtcca ccgttcagtt ggttaacctg aacactgcta ccgcctgcgg
    73201 aggctcctgc gccgccaacc agaatattgc cctgcgccag cgcatacacc tgactgtcaa
    73261 cgcccttaag cggagtcatc aacaacgtac ctccacgcaa gcttttggca ttacccatgg
    73321 aagaaaccac cacatcgatg gtttgcccct gacgtccaaa cggaggaagt gacgctgtca
    73381 ccattaccgc agcgacgttt tttagctgca tattggtgcc cgtcggaacg gtaattccca
    73441 gctgtgagag catgttatta agcgtttgtg tggtaaacgg cgtctgggtt gtctggtcac
    73501 cagtgccatc cagccccact accaagccat agccaatcag tgagttttgc cttaccccct
    73561 gaacactggt gagatcgcga atacgctcag cctgagccgc cgtcgtaacc agtagaagaa
    73621 ttaatgcaga gagaaattta atcaccacag cctcacttac attggcgaca ggttaaggaa
    73681 gaaacgctgc aaccagccca tattttgcgc ttcgttaatg tagccattgc ctacgtattc
    73741 aatacgcgca tccgccacct gagtagacgg tacggtattg ctgccgctga tagtgcgtgg
    73801 attaaccacg cccgagaagc gaataaattc ggtaccctga ttaatggcaa tctgtttttc
    73861 acccaccaca tgcaggttgc cgttgaccag tacctggtca accgtcaccg tcaacgtgcc
    73921 gctaaaggta ttgctggcat tggccccgcc ctttccgttg aacgtgttac caccggaggc
    73981 ttcgacatcg gcacgagcgt taccaaacag cccctgcaaa tagcgcggca cagtatcaaa
    74041 gccaaaatta gttttaccgt cacggctggc attcgcagag gagcttttgc tggcgctgac
    74101 gttctcctgc aacacgatgg tcagcgtatc gccaatattg cgtggtcgac gatcttcaaa
    74161 cagcggttga tagccatagt taatcggctg agcagactgg aaaatagaac cgttggcgac
    74221 gggcgtcgga ccgggaaccg gttgtgcact ggtcgccccc tgcaccagcg gcgtggaggg
    74281 tatccaggcg cagccggtta gtgaaagcac caacaagctg gaaatggcat aagtatgcgc
    74341 agcgtttttt tgcatggcta tcatcttcaa aaatcagtaa accggtgaac ctgccaccgg
    74401 ttatgcctta gagttgcgtc agtttttgca gcatctgatc ggtggtggac accgctttac
    74461 tgttgatttc gtaagcgcgt tgcacctgaa tcatattgac cagttcttcc gccacgttga
    74521 cgttagacgt ttcaacatac ccttgataca gcagtcccgc gccgttcagg cccggcgtgc
    74581 tttcgttcgg tgcaccggag gattgcgttt cggtgtagag gttttcgcca atgctctcca
    74641 gtccggtatc attcataaag gtggtgagat tgagctgccc aacctgaacc ggagctgcct
    74701 ggccttgttg ggttacgctg accacgccat cacgaccgat ggtgatactt aacgcattcg
    74761 ccggaatggt gatcgctggc tgcacctgaa aaccaccagc cgtcaccagc tgcccgttct
    74821 gatccacctg gaaagagccg tcacggatat aggctgatga gccatctggc aacattacct
    74881 ggaaaaagcc ctgcccttta atcgcgacat ctttgctgtt attggtctgc gacagattgc
    74941 cctggctgtg taagcgttca gtggcgaccg ggcgtacccc cgtgccgatt tgtaatccgg
    75001 agggtaaggt ggtttgttcg gaagactgtg cccccggctg gcgaatggtt tgataaagca
    75061 gatcttcaaa caccgcgcgc tgacgcttaa aaccgttagt actgacgttt gccaggttgt
    75121 tggcaatgac gtccatattg gtttgctggg cgtcaaggcc cgttttggcg atccataatg
    75181 aactgatcat ataatgtcct gtagtggatt gctgacaagc agagcagaag cacgcgacag
    75241 tgtgaatttc ccctcaccct aaccctctcc ccaaaggggc gagggaatag tccgcgcgct
    75301 tttattgcaa tgtcatcaat cgttcaatta actcatcgac agcagttggt tggcacggcc
    75361 tgcgttatca tcgacgctgc tgatcacctt catctgcatt tcaaaacgcc gcgcgctggc
    75421 aatcatgtcg ctcattgccg caacggcatt gacgttactg ccttccagaa cccccgacat
    75481 cacacgcaag gttggatctg cctgcagtac cggccctcgc gtggcctggg tttctgcgct
    75541 taaacgaaaa atgccgtcgt caccgcgctg cacttcgctg cccgtggctt tcaccagttt
    75601 cagacgccct actggcgcaa ctgtatttgc cggatcgccc ggattgagcg ccgagattgt
    75661 gccatcggca gcaatcgtga tttccgcccc ttccggcaca gcaattggcc ccgcctcgcc
    75721 tatcaccgga tgcccctgaa ttgtcagttg cccggtggga tcaacctgaa tgctgccatt
    75781 acgcgtatac ccttcgctgc cgtcagcggt ctgcacggcc agccatcctg ctgcaacgca
    75841 acgtccagcg ggcgagaggt gtaatccatc ttgccgggcg tcatgtctgc acccggtgtt
    75901 gacgccgtta ccagcgtgcg cgtgggcagt gaaagccctt ccaccggcac tgcgcgcaag
    75961 gcgtttaact gcgcgcgaaa accgggcgta gaggcattgg ccagattact ggcggttacc
    76021 gcctgttgat tcagcgtctg gctggctgct cccatcgcag tataaattgc gtgatccatt
    76081 gagctatccc gtcagcgatt agcgtaagtt aacccgcgtg ttgaggatct ggtcctgggt
    76141 tttgatggtc tgggcgttag acttatagtt acgctgggca acgatcatat tgaccagttc
    76201 tttactgaga tcgacgttgg acgcttccag cgcaccgttg gtcagagtgc caaagtttcc
    76261 cgtcccggct gtccccaaca gcgccacgcc agaagattgc gtcgcagacc agacgttgtc
    76321 gccttcggat gccagacctt cgttgttggc aaagttcgcc agtacaatct gccccagcag
    76381 ttgggtttgt tcgttggaat tgttgccgac aaccgtaccg tcatcattga tttgataact
    76441 caccagatcg cccggtttgt agccattctg ggtggttgcc acaatgttgt tagcgccggt
    76501 tgcgatatta ttcgccatcg caccatccac taatgtgcca ttagcattaa attccagtgt
    76561 tgtcgctgtc ttcgcaatgc tgtttggatc actgctatcc tgggtgtaga cctgccagtt
    76621 attatccccg gtcttcacaa aatagacgct catgtcatga gcattaccct gactgtcgaa
    76681 aacagtcacc gaaccttttt tgttatagct atccgcattg ctggcgctga atggcgtaac
    76741 agtaggaagc ggatcactgg aattcaggtt gatctgcatc gacgccgtgg tggtagtttt
    76801 cgctgccatc agggtattcg ggatcgaaat attggtcgga ttcgcccctt gctgaatagt
    76861 cggcggcgta ccggttaccg ggtaacccgt cagctgtaaa ccttgcgtat tcaacaggtt
    76921 acggttttca tccagcttaa attgtccgtt acggctgtag aacaccgaac cgttgctgtc
    76981 taccagacgg aaaaaaccgt tctggctgat agcaacgtcc agacctcgcc cggtgttggt
    77041 ggtcgtgcca tcggtaaagt cctgagtgat accggcaact tttaccccca gtcccacttt
    77101 cgaaccggca aacatatcgg caaaagaggc cgtgcctgat ttaaagccgt aggtggcgga
    77161 gttggcgata ttgttgccaa taacatcgag gttggtggcg gcagcgttta atccgctaac
    77221 cgcttgagaa aaggccatga ctgactcctg aaatgtgaag gcttaaatta tctgccgtac
    77281 ttcgtcgagg gtggtggtgc cgtaagtgcc gagatccagc gtattaccgc tgttgccgcg
    77341 gatcacaccc tgcaccagag caaactgcag cggctgggca accagttgtg taccaccgtt
    77401 actggcgcta atcgctacat tgtaagaacc gttcggcgca gtgctgccat cagtcaacga
    77461 accgtcccag gtgaaactgt gaactccggc ggtcagttca ccaatatcaa tggtgcgcac
    77521 aaccgcgcca tttttatcgg tgatggtggc cgtaactttg tctgccgcct gttgcagctc
    77581 aacaccaaac ggcgtggtcg tggtcacagc cccttcttca ctgccggttc ccgcaagaac
    77641 agttgtgccg gggatcatca cgccgtgacc gatcaggtta ctggcctgta acgactggct
    77701 gttatcaatc tgtccggaaa tagatccgag cgtggtattg agtttttcaa tcccgctgac
    77761 cgtgctgatt tgtgccaatt gcgacgtcag ctcgttgttt tccattggat tggtcgggtc
    77821 ctggtttttc agctgcgcca ccagcaaagt cagaaaactg ctttgtaaat ctgcggcgtt
    77881 gctgcccgtg agcgaactac tgctggtggt actgacgccg gtatttgtcg gatcggtggt
    77941 ggttaccgca atggacatag ctttctcctt tattgaccga gcgtaagggt tttcagcatc
    78001 atgcttttca ccgtgttgag cacttcaaca ttggcctgat agctgcgtga cgccgacatg
    78061 gtgttaacca tctccccgac gacatcaacg ttcggcattt ttatgtagcc ctttgcatct
    78121 gccagcggat tacctggttc ataaaccagt ttgtccgggg cctgactttc ttaacatcgg
    78181 caacctttac gccgcctgtc gcagcacctg gtgcagcgtt aacctggaat accacctgtt
    78241 ttgcccgata tggctgtcca tcaggaccgg tcacgctatc agcatttgcc agattactgg
    78301 ccgccacgtt caggcgctgg gactgggcag ttaacgccga cccggcgata tcaaaaatat
    78361 tcagcagtgc catccgttaa tttccgctct gtaaaacgtt catcatgcct ttgatttgcc
    78421 cgctcaacgc gctaaggctc atctggtatt gcaggctgtt atcggcaaac tgggtgcgtt
    78481 cgcgatccat atcgacggta ttaccgtcaa gcgaaggctg gtccggaata cggtattgca
    78541 gttctgcggt aggaggcgtc agcgcctgcg ccgggatgtg ctgcgttgag gtcatcgtca
    78601 gtgcaaccac actggttgca tcccgtccac gttgcatgac ttttttaagt tcactggcaa
    78661 aatcgatatc gcgcgcctga taaccagggg tatcggcatt ggcgatgttt gctgccagca
    78721 cttcctgacg ctgggcgcgc agattgagcg cctcttgttg aaaacgtaag gcggcgtcga
    78781 gcttatcgag catatctcct ccgcaggtat caaaattctg ccatcagctt aaatgccttt
    78841 acgcgcgcgt tatcggcgga ataaacgcaa aatgggtcgc tatttatgcc gttgatggtc
    78901 attgcggaca ggtacaattc acgttgtaga aatggctggg ggcgaaaatg ctgacaataa
    78961 aacgtagcgt ggcgatcatc gcgatactgt tcagtccgtt aagtacggcg agcaatctca
    79021 catcgcaatt gcacaacttt tttagcgccc aactcgcggg ggtaagtgat gaggttcgtg
    79081 tttctattcg tacagcgccc aatctactac cgccatgcga gcagccattg ctttcgatga
    79141 gcaataattc ccgcctgtgg ggcaatgtga atgtgttggc acgctgcggt aacgacaaac
    79201 gatatttaca ggttaatgtg caggccacag gaaattatgt ggttgccgcg atgcctattg
    79261 tgcggggagg aaagctggaa gctggcaatg tcaaactgaa acgtggacgc ctggataccc
    79321 tgccaccgcg tacggtgctg gatatcaatc aacttgttga tgccgttagc ctgcgcgatc
    79381 tatcacccga tcaacctatc cagttaaccc agtttcgcca ggcatggcgg gtaaaagcgg
    79441 gacaacgcgt caatgtgatc gccagcggtg atgggtttag cgccaacgca gaaggtcagg
    79501 cgctgaacaa tgcagccgtc gcacagaatg cgcgggtgcg catggtatcg gaacaggtag
    79561 tcagcggcgt tgttgatgca gatgggaata ttcttataaa cctgtaagct gttaaagatt
    79621 acccgtccct tgccgataaa taagcaacac atgataaaag cgccctcaat gaggaataaa
    79681 ccatgagtat tgatcgcact tcgcctctga agcctgtaag caccgttcaa ccgcgcgaaa
    79741 ccaccgacgc gccggtaacg aacagccggg cggcaaaaac aaccgcctcc accagcacca
    79801 gtgtgacgtt aagcgacgcg caagcaaaac tgatgcaacc cggcagcagt gatatcaatc
    79861 ttgaacgtgt cgaagcgtta aaactggcga ttcgtaacgg tgaactaaaa atggacaccg
    79921 gcaaaattgc cgatgcgctg atcaacgaag cgcagcaaga cttgcagagt aactgatcgt
    79981 atgacacgtc ttgcagagat cctcgaccag atgtccgctg tgcttaacga tctcaaaacg
    80041 gtaatggatc aagagcagca acatctctct atggggcaga tcaacggcag ccagttgcaa
    80101 tggattacag aacaaaaaag ctcactgctg gcgacgctgg attacctcga acagttacgc
    80161 aagaaagaac ccaatacagc aaatagcgtt gatattagtc aacgctggca ggaaattact
    80221 gtgaaaacgc agcaactacg ccaaatgaat caacataacg gctggttact ggaaggacag
    80281 attgagcgca atcaacaggc gctggaaatg ttgaaaccgc atcaggaacc gacgctatat
    80341 ggggcgaacg gtcagacctc aacaccccat cgcggcagta aaaagatttc gatctgaata
    80401 ttatctccgg cctgcactgc aggccggaat gcattgttac accgtccggc gggcaaattc
    80461 tttaactttg aagcccagta ccgccagtgc agcgaagtac gcggcaatcc ccgccagcac
    80521 gaccgccatt aaacgcagta agcgccaggg catggtaccc aatgaccact ccggcatgat
    80581 atgtaacata cctaaaagca cgccagacat caccagtacc gccaccacca gacgcaacag
    80641 aaacgccatc cagccgggtt gcggagtaaa gattttctgc ttacgcaact gccagtaaag
    80701 cagcgaagca ttcagacacg ccgccagacc aatagaaagt gacagcccgg catgtttcaa
    80761 cggaccaata aacgccaggt tcatcaattg cgttaaaatc agcgtaacga tggcaatttt
    80821 cactggcgtt ttaatgtcct ggcgggaata aaagccagga gccaacactt tcactacaat
    80881 caggccaatc aaacccaccg agtaggcaat taacgcccgc tgggtcatca gcgcatcaaa
    80941 cgcggtaaat ttaccgtact ggaacagcga aacggttaac ggacctgaaa gaatgcccaa
    81001 cgcaaccgca ctcggcagtg ccaacaggaa acaaagacgc aacccccagt ccatcaaacg
    81061 gttgtattca tcatgattgc cactggcaaa acttttcgac agcgacggca gcaaaatggt
    81121 gccaagcgcc acgcccagca caccggaggg aaactccatt aagcggtcgg cgtaatacat
    81181 ccaagacacc gaaccggaag caagaaacga ggcaaaaatg gtgttgatga ttaaggagat
    81241 ctggctcaca gagacgccaa ggatcgccgg tcccatctgt ttcaccacgc gcattgctcc
    81301 agcatcgtgg aagttaatgc gcggcaggac cagcatgccg atcttcttca ggtgcggtag
    81361 ctgatacacc agctgcagaa tgccgccgac cgtaacagcc cacgccagcg ccagcaccgg
    81421 tgggttaaag tacggtgcgg caaacagcgc gaaaccaatc atgctgatgt taagcagtgt
    81481 tggcgcaaac gccggaatcg agaagcggtt ccacgtattc agaatcgctc ccaccagcga
    81541 cgccagggag atcagcaaga tatagggaaa ggtaatcttt agtagctggc tggtcagggc
    81601 aaatttgtca gctgtgtcag cgaagcctgg cgcggtcacc atgatcaccc acggtgcggc
    81661 gagcatgcca gcgaccgtca caaccgccag cgcaagtgtc agcaggccag aaacataaga
    81721 gacaaagacc cgcgtggcgt cttcaccctg cttactttta tattccgcca gaatcggtac
    81781 aaatgcctgg gaaaatgccc cttcggcaaa gatacggcgt aacaagttag gaagtttaaa
    81841 agcgacgaaa aaggcgtcgg ttgccatccc tgcgccaaag attctggcga caattgcgtc
    81901 tcgtgcgaag ccaagcacac gcgaaaacat ggtcatcgag ctgacggcgg ccagcgattt
    81961 taataaattc atcggtgttc taatccggac ccacgggcaa aacgcctgca aggcaggcgc
    82021 tgaaagaggc gaatagtcta cctagattat ggtgaattgc taccgccaga tgttacaggg
    82081 tttattcact catcgcatcg cgccagatct tgtcaacgat acgttgcgcc agcactgcct
    82141 attcgccggc ggtttgcgga actgtctgat tttacacgca ttcaatgaag tgccgcgcac
    82201 agccgacaaa tccacgttgc tcaagagtgc tctgccaacc aggaatcggt ttattcacta
    82261 caccctgccc gcgctcctca cgccattcgc gcatatccgt aatgtcgatg agcgcaccgt
    82321 cagtcacggc ctgcacggtt tcacgctgac ttccggcacg gcgatgcata caagtggtga
    82381 tctgcaaagg accggccgaa aaatggtgct cggcaaacag catttcgcca gcgtcgttag
    82441 tcagtagcgt accgccatcc agagaggctt tgccgcccga caaccacagc gcggtatcca
    82501 ccacatgcag ataatcatcc agcaacgtga aataaagatc gtgtggcccg acgctattgc
    82561 tacgatgttt atccattctt agcgaggctg cggtggcgag ttgcgttttt aactcaccgt
    82621 agagtggtgc gaaacgacgg ttaaaaccga ccatcaacgt cagttttttc cgcgccgcca
    82681 gttccaccag cctttcagca tcgcgcagat tttctgccag cggtttatcg acacagacat
    82741 gtacccccgc attgagtaac gtactgacca cgtcaaagtg gctggcggtg ctggagtgca
    82801 caaaaaccgc atcacaactg gcggcaaggc tggataacga atcggcataa ggaatgcgcc
    82861 agctgtcaca aattggcagg gctttggcgc gcgtaggcga ccaggctcct tgtaacgtcc
    82921 agtcagacgc tgccgccagc accggtaacc acgctttttg cgcaatgccg cctaatccca
    82981 ctacgccgat acgtaatttt ttcactttaa tctcccagat gggccagcaa cgaatcaaga
    83041 cgttgtttca gttctgccac ttcgatttcc agggcttcga cgcgggtctg taaatcaccg
    83101 tcaaccatgt tcgacatagc cgttaccgcc ggctgatctt caacctcacc actgaaaaga
    83161 tgcatgtagc ggctttcgcg tttacccggt tcgcgggcca gacgcaccac aaaaggacca
    83221 tcttcgcgat tcgccagttg ttccagcgtc gactccactt ccgccatatc gctgaattca
    83281 tacattcgcg cggcgcggct gcgcagttca cccggcgtct gggcaccacg caataacaac
    83341 gtggtgatta acgccacttc tgcggcgctc agtttcagat cgccaaattc tgaattacaa
    83401 aaacgttgct catatttggt gacccgatta ccaaaaccgc tcactgtgcg tagataatga
    83461 cgtttgacca gattatccag ctgttcctgc acttcggatt cactcagatt catgaccggt
    83521 tcacggttcg ttttctgatt acaggccgtg actacaccat tgactgagag cgggtattgc
    83581 tccggcgtgg tcacctgctt ttccagcaag caaccaatta cgcgagcttc cagggcagta
    83641 agttgatatt tcatgagatt ctccttagcg gccgggcgtc cagtctgggg tagttaatgc
    83701 cgtcagtacg tgatcgcgcc attgtccatc aatcaacaga tagtctttcg catagccttc
    83761 tttttcaaaa cccagtcgcg ccagtaaatc accgctgcgt ttattgtgcg gcatataatt
    83821 agccataatg cgatgaatat gttgggtgcg ctgcatataa cgaatggctg cggtcagggc
    83881 ttcaaacatg agtcctttgc cctgccattt ttgcccaatc gaataaccga gatagcaggc
    83941 atgaaaagag ccgcgaacaa cattggaaaa attggcaacg ccaataatct ctttttcgtc
    84001 cgggtcgaat aagccaaagt agaaagctga accttgttta tgaaattcgt taatcatccc
    84061 tagcctggcc tgccagcctg atggataaca gtggctttcg tcgcgcactg gctcccaggg
    84121 cttgaggaaa tggcgattct ctgcgtaata atccgccaga cgccaggcat cacgatcatg
    84181 caccagacgc acgaccagtc ggtctgtggt taagcgcact tttggcacgt tactgcgata
    84241 gccaaacatt ctatacctac tccttcccgt aacgtcttta atacgctgta agtattacta
    84301 tacctgcgca accagaccct gtgaaaagtc caaaatgcca ttttctgcat tattcacata
    84361 tttgataaaa actctctatc aaagcgacgt tttgataaaa aaatactgtc cccgaagtga
    84421 tgggaaaagt cttagcggca atgcagaata gaaagtgctc atcttttatt tcccgggagg
    84481 ggaaatgtcc cgcgtatcgc aggcgaggaa cctgggtaaa tatttcctgc tcatcgataa
    84541 tatgctggtc gtgctggggt tctttgttgt cttcccgctg atctctatcc gcttcgttga
    84601 tcaaatgggc tgggccgccg tcatggtcgg tattgctctc ggtctacgcc agtttattca
    84661 gcaaggtctg ggtattttcg gcggcgcaat tgccgaccgc tttggtgcca aaccgatgat
    84721 tgttaccggt atgctgatgc gcgccgccgg attcgccaca atgggtatcg cccacgaacc
    84781 gtggctattg tggttttcat gcctgctctc gggactcggc ggcacgttgt ttgatccgcc
    84841 gcgttcggcg ctggtggtga aactaatccg tccacaacaa cgtggtcgtt ttttctcgct
    84901 gttgatgatg caggacagtg ccagtgcggt cattggcgca ttgttgggga gctggctgtt
    84961 gcaatacgac tttcgcctgg tctgcgccac aggggccgtt ttgtttgtgc tatgtgcggc
    85021 gttcaatgcg tggttgttac cggcatggaa actctccacc gtacgcacgc ccgttcgcga
    85081 aggcatgacc cgcgtgatgc gtgacaagcg ttttgtcacc tatgtcctga cgctggcggg
    85141 ttactacatg ctggctgtac aagtgatgct gatgctgcca attatggtca acgacgtggc
    85201 tggcgcgccc tctgccgtta aatggatgta tgccattgaa gcgtgtctgt cgttaacgtt
    85261 gctctaccct atcgcccgct ggagtgaaaa gcattttcgt ctggaacacc ggttgatggc
    85321 tgggctgttg ataatgtcat taagcatgat gccggtgggc atggtcagcg gcctacaaca
    85381 acttttcacc ctgatttgtc tgttttatat cgggtcgatc attgccgagc ctgcgcgtga
    85441 aaccttaagt gcttcgctgg cggacgcaag agctcgcggc agctatatgg ggtttagccg
    85501 tctgggtctg gcgattggcg gcgctattgg ttatatcggt ggtggctggc tgtttgacct
    85561 gggcaaatcg gcgcaccagc cagagcttcc gtggatgatg ctgggaatta ttggcatctt
    85621 cactttcctt gcgctgggtt ggcagtttag tcagaaacgc gccgcgcgtc gtttgctgga
    85681 acgcgacgcc tgatttgata ctcccccctg actcctccat actgaaagtg aactgacagc
    85741 caaatggagg agtcacgtga agctatacat ttacgatcac tgcccttact gcctcaaagc
    85801 ccgcatgatt ttcggcctga aaaatatccc cgtcgaatta catgttctgc tcaacgacga
    85861 cgcagaaaca cccacccgga tggtcggtca aaaacaggtt cccattctgc aaaaagatga
    85921 cagccgctat atgccagaaa gcatggatat cgttcactat gtcgataaac tcgacggcaa
    85981 accgttactg accggcaaac gttcccctgc cattgaagag tggctgcgca aggtcaatgg
    86041 ctacgtcaac aaactgctgt tgccgcgttt tgccaaatcg gcatttgatg agttttctac
    86101 tcccgccgcg cgcaaatatt tcgtcgacaa gaaagaggcc agcgcgggta attttgccga
    86161 cctgctggcc cactctgacg gtctgattaa gaatatcagc gatgatttgc gtgcgctgga
    86221 caaactgatc gtcaaaccga acgccgtgaa tggcgaactt tcggaagatg atattcagct
    86281 atttccgcta ctgcgtaatc tgacgctggt agccggaatc aactggccaa gccgcgttgc
    86341 taattaccgc gataatatgg cgaaacagac acaaatcaat ttgttatcat caatggcgat
    86401 ttaatcctga cccgccgcgg cagcctgtat tcgcgggttg ccgccgtttt cagttttttc
    86461 tgctggcgag tttatcgctg gtgacacatg ctgcgttgtg tcaggatatt gacgcagatt
    86521 tcatgtcgag gactaccatg aacaagtttt tatttgccgc tgcattgatc gtcagtggcc
    86581 tgctcgttgg ctgtaatcaa ctcacccaat acaccatcac cgaacaagaa attaaccagt
    86641 cgcttgcgaa acataataat ttctcaaaag atatcggttt acccggcgtg gctgacgccc
    86701 atattgttct gacaaacttg accagccaaa ttggtcgcga agagccgaat aaggttaccc
    86761 taaccggaga cgccaatctg gacatgaact ccctgttcgg tagtcagaaa gcgaccatga
    86821 aactgaagct gaaagcgctg cccgtgtttg ataaagaaaa aggtgcgatc ttcctgaaag
    86881 agatggaagt ggtcgatgcg acggtacaac cggaaaaaat gcaaacggtg atgcaaacgt
    86941 tgcttcccta tttgaaccag gcattacgca attactttaa ccagcaacct gcttacgtcc
    87001 tgcgcgaaga tggcagtcag ggcgaagcaa tggcgaaaaa actggcgaaa ggcattgaag
    87061 tgaagccagg cgaaattgtc attccgttta cggattaatg acgagggcgc attcgcgccc
    87121 tttatttttc gtgcaaagga aaacgtttcc gcttatcctt tgtgtccggc aaaaacatcc
    87181 cttcagccgg agcatagaga ttaatgactg caccatccca ggtattaaag atccgccgcc
    87241 cagacgactg gcaccttcac ctccgcgatg gcgacatgtt aaaaactgtc gtgccgtata
    87301 ccagcgaaat ttatggacgg gctatcgtaa tgcccaatct ggctccgccc gtgaccacag
    87361 ttgaggctgc cgtggcgtat cgccaacgta ttcttgacgc cgtacctgcc gggcacgatt
    87421 tcaccccatt gatgacctgt tatttaacag attcgctgga tcctaatgag ctggagcgcg
    87481 gatttaatga agccgtgttc accgctgcaa aactttaccc ggcaaacgca accactaact
    87541 ccagccacgg cgtgacgtca attgacgcaa tcatgccggt acttgagcgc atggaaaaaa
    87601 tcggtatgcc gctactggtg catggtgaag taacacatgc agatatcgac atttttgatc
    87661 gtgaagcgcg ctttatagaa agcgtgatgg aacctctgcg ccagcgcctg actgcgctga
    87721 aagtcgtttt tgagcacatc accaccaaag atgctgccga ctatgtccgt gacggaaatg
    87781 aacggctggc tgccaccatc actccgcagc atctgatgtt taaccgcaac catatgctgg
    87841 ttggtggcgt gcgtccgcac ctgtattgtt tacccatcct caaacgcaat attcaccaac
    87901 aggcattgcg tgaactggtc gccagcggtt ttaatcgagt attcctcggt acggattctg
    87961 cgccacatgc acgtcatcgc aaagagagca gttgcggctg cgcgggctgc ttcaacgccc
    88021 caaccgcgct gggcagttac gctaccgtct ttgaagagat gaatgctctg cagcactttg
    88081 aagcattctg ttctgtaaac ggcccgcagt tctatggctt gccggtcaac gacacattca
    88141 tcgaactggt acgtgaagag caacaggttg ctgaaagcat cgcactgact gatgacaccc
    88201 tggtgccatt cctcgccggg gaaacggtac gctggtccgt taaacaataa aaatcgtagc
    88261 ttcctgttgt cattaggtta ttttacctgt ataaataacc agtatattca acagggggct
    88321 attatgcgaa ttgaagtcac catagcgaaa acttctccat tgccagctgg ggctattgac
    88381 gccctggctg gcgaactttc ccgccgtatt cagtatgcgt ttcctgataa tgaaggccac
    88441 gtatcggtac gttatgccgc agcgaataat ttatcggtta ttggcgcaac aaaagaagat
    88501 aaacagcgca ttagcgaaat tctccaggaa acgtgggaaa gcgccgatga ctggtttgtc
    88561 agcgaataat atgcagtgat ttttgttttt gccggatcgc cccggctttt ttattttact
    88621 tacgaatttt gagtacaaaa tttctacact tcctcaaaat aattgcacaa tatgctaaaa
    88681 aaatatgaat gacatgctgt tttattgttc atataaatct taaaagcaga aatttatctg
    88741 ttcccccctt taaaaattcg gtgaataccc ttacttattg gtatactgaa gttgcttcag
    88801 ataaaaacgc attgaacctc gaataacgtt gtctagtaac acgaattagg gggccatgat
    88861 ggaaaaaaat aatgaagtca ttcagactca tccgctcgta gggtgggaca tcagcaccgt
    88921 tgatagctat gatgcgctga tgttgcgttt gcactaccag accccaaata agtccgagca
    88981 ggaagggact gaagttggtc agacgctctg gttaaccact gatgttgcca gacaatttat
    89041 ttcgatatta gaagcaggaa tcgccaaaat tgaatccggt gatttcccgg taaacgagta
    89101 tcggcgtcat taacatgaca tgaccatcca cccaaaaggc acctcaccgg tgcctttacc
    89161 atttctgggt tgtatatctg cattcagtta attactctgc ttaaaccatg agagcagaga
    89221 gaaaaaaatg aaatacgatc tcatcattat tggcagcggt tccgtaggcg ctgccgccgg
    89281 gtattatgca acccgcgccg gtttaaacgt gctaatgacc gacgcccata tgccaccgca
    89341 tcaacacggc agccaccacg gcgatacgcg attaattcgc catgcttatg gtgaaggcga
    89401 aaagtatgtc ccgttggtcc tccgcgcgca aacgctgtgg gatgaacttt cccgccacaa
    89461 cgaagatgat cccatttttg tacgctctgg tgtcattaac ctcggcccgg ctgactccgc
    89521 atttctcgcc aacgtcgccc acagcgccga acagtggcaa ctcaacgttg aaaagctcga
    89581 tgcgcaaggg attatggccc gctggccaga aatacgcgtc ccggacaact acatcggctt
    89641 atttgagact gattccggtt ttttgcgcag cgaactggcg attaaaacct ggatccaact
    89701 ggcgaaggaa gcgggctgtg cgcaactgtt caactgcccg gtcaccgcaa ttcgtcatga
    89761 cgatgatggc gtaactattg aaacggttga cggtgagtat caggcgaaaa aagcgattgt
    89821 ctgcgcggga acatgggtaa aagacctgct cccggagctg cctgtccagc ccgtacgtaa
    89881 agtatttgcc tggtatcagg ccgatggccg ctatagcgtg aagaataaat tcccggcgtt
    89941 taccggtgaa ctgcccaatg gcgatcaata ttatggtttt ccggcagaaa acgacgcgtt
    90001 gaagattggc aaacataacg gaggccaggt tatccattca gcggatgaac gtgttccgtt
    90061 tgcggaagtg gccagtgatg ggtcagaagc cttcccgttc ttgcgcaatg tattgccggg
    90121 tatcggttgc tgcctgtacg gcgctgcctg cacctatgat aattcgcctg acgaagattt
    90181 tattatcgat accctacccg gccacgataa tacactgctc attaccggcc tgagtgggca
    90241 cggttttaaa tttgcgtcag ttttagggga aatagctgcc gattttgcgc aagaccaaaa
    90301 aagcgatttt gatttgacgc cattcaggct ttcccgcttc caataatcat cattacggcc
    90361 tccagaatga ggccgttttt acaggagtat ttaatgcgtc ggctgttgca ctatctcatc
    90421 aataatattc gcgagcatct gatgctttat ctttttctct ggggattgtt ggcaattatg
    90481 gatctgatct atgtgtttta tttttagttt tccgcactga cataaactga caaatattta
    90541 atctcccttt catttagcgt gaaaaattga atgagattat tcctgcttct acagcactca
    90601 ccagacatct ctgatgctga acccactaaa cttatgaaga ttctttcatt attaattcaa
    90661 aattattgaa ataaaaataa atatcattca atttgttatt tttgtgttgc aattcattct
    90721 ccgacacgcc attttctttc tctcgcaaat tcgtattgga tgcacttatg tcattcacaa
    90781 atacccctga acgctatggt gttatatctg cggccttcca ttggctaagc gcaattattg
    90841 tctacggcat gtttgcttta ggtctgtgga tggtcacgct cagttattac gatggctggt
    90901 atcacaaagc acccgaactg cataaaagca tcggtatttt gttaatgatg gggctggtta
    90961 ttcgcgttct gtggcgtgtc atatctccac cgccgggtcc gctgccgagt tattcgccca
    91021 tgactcgtct tgccgcaaaa gctggacatc tcgcattgtg cctattgctg ttcgccatcg
    91081 gcatcagcgg ctatctgatc tcaactgccg atggtaaacc gatcagcgtt tttggctggt
    91141 ttgacgtccc cgcgaccctt gccgacgctg gcgcacaggc agactttgcc ggtgccctgc
    91201 atttttggct cgcgtggagc gtcgtagtac tgtctgttat gcacggattt atggccctga
    91261 agcatcattt catcgataaa gacgacactc tgaagcgcat gctgggaaag tcgtcatctg
    91321 actatggagt ataaaaaatg aaaaaaagcc tgcttggttt aaccttcgcg tccctgatgt
    91381 tctctgccgg ttcagcggtt gccgccgatt acaaaattga caaagaaggt cagcacgcct
    91441 ttgttaactt ccgcatacag catcttggct atagctggtt atatggcacc tttaaagatt
    91501 tcgacggtac ttttaccttt gatgaaaaaa atccggctgc cgataaagtg aatgtgacaa
    91561 ttaacaccac cagcgtcgat actaaccacg ccgaacgcga taaacatctt cgcagtgcgg
    91621 attttctcaa taccacaaaa tatccacagg caacattcac ctccaccagc gtgaagaaag
    91681 acggtgacga actggatatt accggcgatc tgacgctgaa tggcgtaacc aaacccgtca
    91741 cgctggaagc gaaattaatt ggtcagggcg acgacccatg gggcggtaaa cgtgcaggct
    91801 ttgaggccga aggcaaaatt aagctcaaag acttcaatat caagacagat ttaggtccag
    91861 cttctcagga agtggatctg attatttcag tggaaggcgt acagcagaag taagagtgaa
    91921 atgttgccgg atggcacgca tccggcaatg atatttattc tgttggatca ggaataccca
    91981 gtgttgtatt cagacgtcca cgagacttat taaagatctt attgccattt tcacgtcctg
    92041 cccgacggcg tcgctgttct tctggcggta acgcgctttc ttcgcagcaa atctcactac
    92101 aacaaccttt gtatttttcc gcgcatactg gacactgaat aaacagcagg tggcagccat
    92161 catttttaca gttggtatgg ctgtcgcacg gcgcgccgca ctggtggcaa tgcgcgataa
    92221 tctcatccga tatacgttcg cccatccgct cgtcaaaaac aaaatttttg ccaataaaac
    92281 gcaccggcaa gccctgctcg cgcgccttac gggcgtattc aataattccg ccctcgatat
    92341 gccagacttt gttgaatccg ttatgtttca tccaggcact ggccttttca caacgaatgc
    92401 cgccggtgca gtacatgacg atttttttat ctttatgtgc ctgcatcatc tcgactgctt
    92461 ttggcagctg ctcacggaag gtatctgccg gaatttcgag cgcgttttca aagtgcccca
    92521 cttcatactc atagtggtta cgcatgtcga taaatagtgc atcgggatcg tcaagcatgg
    92581 cgttcacttc cgccgcttgc agatactcac caacattgct ggcatcaaag tgaggatcgt
    92641 caataccgtc ggcaacaatg cgatcgcgta ccttcatgcg cagtacccag aaggatttcc
    92701 cgtcatcatc caacgcgata ttcaggcgta aaccctctaa agccgggtcg aaggcataga
    92761 gctgcgcgcg aaatgtttca acattgctcg caggtacgct gatttgcgcg ttaatgccct
    92821 catgcgccag atacactcgc ccaaaaacat tcagcgcggt aaacagctga tataaagcgt
    92881 cacgggtcgc cttaggatcg gcgatgtgga aatacttgta aaacgaaatg gtggttcgcg
    92941 gttcgctctc agccaacatt ttggctttta acgcgtcgtt ggaaatgcgg ttgtgtaaca
    93001 ctggcatggt gtacggttcc tgcgagatgg gaaagtaaaa atccgcggca tgatatagca
    93061 attatcgata attaacatcc acacatttta cgctacattt gcgcattaaa aattatttgt
    93121 tatttacaag cgcggcaatt tcgcccagtc ttcagccaca aattttggtt gcgggcgaaa
    93181 aaatgcgaca atacatacaa ttgcccgtat aggttgaaaa acaggattga tatgacgaat
    93241 ctacccaagt tctccaccgc actgcttcat ccgcgttatt ggttaacctg gttgggtatt
    93301 ggcgtacttt ggttggtcgt gcaattgccc tacccgatta tctaccgcct cggttgtgga
    93361 ttaggaaaac tggcgttacg ttttatgaaa cgacgcgcaa aaattgtgca tcgcaacctg
    93421 gaactgtgct tcccggaaat gagcgaacaa gaacgccgta aaatggtggt gaagaatttc
    93481 gaatccgttg gcatgggcct gatggaaacc ggtatggcgt ggttctggcc ggaccgccga
    93541 atcgcccgct ggacggaagt gatcggcatg gaacacattc gtgacgtgca ggcgcaaaaa
    93601 cgcggcatcc tgttagttgg catccatttt ctgacactgg agctgggcgc gcggcagttt
    93661 ggtatgcagg aaccggggat tggcgtttac cgcccgaacg ataatccact gattgactgg
    93721 ctacaaacct ggggccgttt gcgctcaaat aaatcgatgc tcgaccgcaa agatttaaaa
    93781 ggcatgatta aagccctgaa aaaaggcgaa gtggtctggt acgcaccaga tcatgattac
    93841 ggcccgcgct caagcgtttt cgtcccgtta tttgccgttg agcaggctgc gaccacgacc
    93901 ggaacctgga tgctggcacg gatgtccggc gcatgtctgg tgcccttcgt tccacgccgt
    93961 aagccagatg gcaaagggta tcaattgatt atgctgccac cagagtgttc tccgccactg
    94021 gatgatgccg aaactaccgc cgcgtggatg aacaaagtgg tcgaaaaatg catcatgatg
    94081 gcaccagagc agtatatgtg gttacaccgt cgctttaaaa cacgcccgga aggcgttcct
    94141 tcacgctatt aaatctccca tgccggatgc ttcagaatgg catccggcat taccacagca
    94201 aatccccctg atttagcgat aaaagctctc tggattgcgc cccctggaag tcgggcgcat
    94261 aattagtgtg tttatctttt cttcttatgt tcaccgcgcc tggcgcacca acagcggatt
    94321 gctatgtcac cctgtgaaaa tgacacccct ataaactgga aacgaaacct gatcgtcgcc
    94381 tggctaggct gttttcttac cggggccgcc ttcagtctgg taatgccctt cttacccctc
    94441 tacgttgagc agcttggtgt taccggccac tccgccctga atatgtggtc cggtattgtc
    94501 ttcagtataa tgggttccta aacacatcat tttaggtatt gaatatgaga cgggcaatca
    94561 gctacatcag attttcaagt gaacgacaac tgaaagggga ctctgtacgc cgacaatcta
    94621 agctggttac tgactggctt gataagaacc ctgagtttta cttggattca tccttgagct
    94681 ttaaagattt ggggaaaagt gcttttagtg gtaagcacct gaaaggtggt ctaggtgact
    94741 ttttaaccgc tatcgagaaa gggcttgtta aagctggtga tacgctacta atcgagagtt
    94801 tagaccgtct gtcacgtcag gatattgata ttgcttctga gctattacgc cgtatcttaa
    94861 gggctggtgt agatgtagtc acactgagcg atggagaaca ttacacacga gaaagtttaa
    94921 aagacccgtt agccctgatt aaaagcattc tgataatgca gcgggcgcat gaggaaagtt
    94981 tgagaaagtc tgagcgtgtc caagctgcat ggaaccgtaa aaaagaactc atctccgaag
    95041 gtatcaaggt atctaggcgt tgtcctgcat ggttaaggct gaatgatgac cgtcgaacat
    95101 tcacaatcat tcctgataaa gtggaagttg ttaaacgagc atttgacctg agattacagg
    95161 gtttaagctt ttgggctatc accagaactt taaacgatga aggtcacttg tcattgaatc
    95221 aatacacacc caaacaaaag gggtggtctg atactgctgt aaagaaactg ctcagaaatc
    95281 gtgctgtgat tggatgtttc acacctgctg gacgtgaaga agttcagggt tactaccccg
    95341 caataatcag tgagtcacta ttctatcgag tacagcaact gaacacaggg caatatggta
    95401 gggcttctgt atcaagtaat ccgctatcag tcaacttgtt caggg
//
LOCUS       DAJTQQ010000003        75360 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000003 DAJTQQ010000000
VERSION     DAJTQQ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.003"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(126..332)
                     /locus_tag="OO309_000238"
     CDS             complement(126..332)
                     /locus_tag="OO309_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR5845529.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            645..734
                     /gene="kdpF"
                     /locus_tag="OO309_000239"
     CDS             645..734
                     /gene="kdpF"
                     /locus_tag="OO309_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR5845530.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            734..2407
                     /gene="kdpA"
                     /locus_tag="OO309_000240"
     CDS             734..2407
                     /gene="kdpA"
                     /locus_tag="OO309_000240"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR5845531.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2430..4477
                     /gene="kdpB"
                     /locus_tag="OO309_000241"
                     /pseudo
     CDS             2430..4477
                     /gene="kdpB"
                     /locus_tag="OO309_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4486..5058
                     /gene="kdpC"
                     /locus_tag="OO309_000242"
     CDS             4486..5058
                     /gene="kdpC"
                     /locus_tag="OO309_000242"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR5845532.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5051..7731
                     /gene="kdpD"
                     /locus_tag="OO309_000243"
                     /pseudo
     CDS             5051..7731
                     /gene="kdpD"
                     /locus_tag="OO309_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            7728..8413
                     /gene="kdpE"
                     /locus_tag="OO309_000244"
                     /pseudo
     CDS             7728..8413
                     /gene="kdpE"
                     /locus_tag="OO309_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            8688..8792
                     /locus_tag="OO309_000245"
     CDS             8688..8792
                     /locus_tag="OO309_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR5845533.1"
                     /translation="MENNNRLMPHIRRTTHIMKFTHRNSFDFHFFNAR"
     gene            9103..11300
                     /gene="speF"
                     /locus_tag="OO309_000246"
                     /pseudo
     CDS             9103..11300
                     /gene="speF"
                     /locus_tag="OO309_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11297..12616
                     /gene="potE"
                     /locus_tag="OO309_000247"
     CDS             11297..12616
                     /gene="potE"
                     /locus_tag="OO309_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR5845534.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12672..14312)
                     /gene="pgm"
                     /locus_tag="OO309_000248"
     CDS             complement(12672..14312)
                     /gene="pgm"
                     /locus_tag="OO309_000248"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR5845535.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14338..14883)
                     /gene="seqA"
                     /locus_tag="OO309_000249"
     CDS             complement(14338..14883)
                     /gene="seqA"
                     /locus_tag="OO309_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR5845536.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15068..15832
                     /gene="ybfF"
                     /locus_tag="OO309_000250"
     CDS             15068..15832
                     /gene="ybfF"
                     /locus_tag="OO309_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5845537.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            15972..16265
                     /gene="ybfE"
                     /locus_tag="OO309_000251"
     CDS             15972..16265
                     /gene="ybfE"
                     /locus_tag="OO309_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR5845538.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16405..16935
                     /gene="fldA"
                     /locus_tag="OO309_000252"
     CDS             16405..16935
                     /gene="fldA"
                     /locus_tag="OO309_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR5845539.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17145..17231
                     /gene="uof"
                     /locus_tag="OO309_000253"
     CDS             17145..17231
                     /gene="uof"
                     /locus_tag="OO309_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR5845540.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17224..17670
                     /gene="fur"
                     /locus_tag="OO309_000254"
     CDS             17224..17670
                     /gene="fur"
                     /locus_tag="OO309_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR5845541.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(17754..18080)
                     /locus_tag="OO309_000255"
     CDS             complement(17754..18080)
                     /locus_tag="OO309_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5845542.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18117..19523)
                     /gene="chiP"
                     /locus_tag="OO309_000256"
     CDS             complement(18117..19523)
                     /gene="chiP"
                     /locus_tag="OO309_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR5845543.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20102..21766)
                     /gene="glnS"
                     /locus_tag="OO309_000257"
     CDS             complement(20102..21766)
                     /gene="glnS"
                     /locus_tag="OO309_000257"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR5845544.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(21969..23915)
                     /gene="nagE"
                     /locus_tag="OO309_000258"
     CDS             complement(21969..23915)
                     /gene="nagE"
                     /locus_tag="OO309_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR5845545.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24248..25048
                     /gene="nagB"
                     /locus_tag="OO309_000259"
     CDS             24248..25048
                     /gene="nagB"
                     /locus_tag="OO309_000259"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5845546.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25108..26256
                     /gene="nagA"
                     /locus_tag="OO309_000260"
     CDS             25108..26256
                     /gene="nagA"
                     /locus_tag="OO309_000260"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5845547.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26265..27485
                     /gene="nagC"
                     /locus_tag="OO309_000261"
                     /gene_synonym="nagR"
     CDS             26265..27485
                     /gene="nagC"
                     /locus_tag="OO309_000261"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR5845548.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27533..28285
                     /gene="nagD"
                     /locus_tag="OO309_000262"
                     /pseudo
     CDS             27533..28285
                     /gene="nagD"
                     /locus_tag="OO309_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            28682..30346
                     /gene="asnB"
                     /locus_tag="OO309_000263"
     CDS             28682..30346
                     /gene="asnB"
                     /locus_tag="OO309_000263"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR5845549.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            30725..30801
                     /locus_tag="OO309_000264"
     tRNA            30725..30801
                     /locus_tag="OO309_000264"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30759..30761,aa:Met,seq:cat)
     gene            30810..30894
                     /locus_tag="OO309_000265"
     tRNA            30810..30894
                     /locus_tag="OO309_000265"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30844..30846,aa:Leu,seq:tag)
     gene            30918..30992
                     /locus_tag="OO309_000266"
     tRNA            30918..30992
                     /locus_tag="OO309_000266"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30950..30952,aa:Gln,seq:ttg)
     gene            31027..31101
                     /locus_tag="OO309_000267"
     tRNA            31027..31101
                     /locus_tag="OO309_000267"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31059..31061,aa:Gln,seq:ttg)
     gene            31117..31193
                     /locus_tag="OO309_000268"
     tRNA            31117..31193
                     /locus_tag="OO309_000268"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31151..31153,aa:Met,seq:cat)
     gene            31242..31316
                     /locus_tag="OO309_000269"
     tRNA            31242..31316
                     /locus_tag="OO309_000269"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31274..31276,aa:Gln,seq:ctg)
     gene            31354..31428
                     /locus_tag="OO309_000270"
     tRNA            31354..31428
                     /locus_tag="OO309_000270"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31386..31388,aa:Gln,seq:ctg)
     gene            complement(31582..32757)
                     /gene="ubiF"
                     /locus_tag="OO309_000271"
     CDS             complement(31582..32757)
                     /gene="ubiF"
                     /locus_tag="OO309_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR5845550.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            32903..34327
                     /gene="miaB"
                     /locus_tag="OO309_000272"
     CDS             32903..34327
                     /gene="miaB"
                     /locus_tag="OO309_000272"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR5845551.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            34480..35520
                     /locus_tag="OO309_000273"
     CDS             34480..35520
                     /locus_tag="OO309_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR5845552.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            35517..35984
                     /gene="ybeY"
                     /locus_tag="OO309_000274"
     CDS             35517..35984
                     /gene="ybeY"
                     /locus_tag="OO309_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR5845553.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            36074..36952
                     /gene="corC"
                     /locus_tag="OO309_000275"
     CDS             36074..36952
                     /gene="corC"
                     /locus_tag="OO309_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR5845554.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            36977..38515
                     /gene="lnt"
                     /locus_tag="OO309_000276"
     CDS             36977..38515
                     /gene="lnt"
                     /locus_tag="OO309_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR5845555.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            38912..39820
                     /gene="gltI"
                     /locus_tag="OO309_000277"
     CDS             38912..39820
                     /gene="gltI"
                     /locus_tag="OO309_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR5845556.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            39990..40730
                     /gene="gltJ"
                     /locus_tag="OO309_000278"
     CDS             39990..40730
                     /gene="gltJ"
                     /locus_tag="OO309_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR5845557.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            40730..41404
                     /gene="gltK"
                     /locus_tag="OO309_000279"
     CDS             40730..41404
                     /gene="gltK"
                     /locus_tag="OO309_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR5845558.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            41404..42129
                     /gene="gltL"
                     /locus_tag="OO309_000280"
     CDS             41404..42129
                     /gene="gltL"
                     /locus_tag="OO309_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR5845559.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            42247..43182
                     /gene="rihA"
                     /locus_tag="OO309_000281"
     CDS             42247..43182
                     /gene="rihA"
                     /locus_tag="OO309_000281"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR5845560.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            43266..44936
                     /gene="hscC"
                     /locus_tag="OO309_000282"
                     /pseudo
     CDS             43266..44936
                     /gene="hscC"
                     /locus_tag="OO309_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(44996..46447)
                     /gene="djlC"
                     /locus_tag="OO309_000283"
     CDS             complement(44996..46447)
                     /gene="djlC"
                     /locus_tag="OO309_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR5845561.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPQCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(46444..47151)
                     /locus_tag="OO309_000284"
     CDS             complement(46444..47151)
                     /locus_tag="OO309_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5845562.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            47253..47807
                     /locus_tag="OO309_000285"
     CDS             47253..47807
                     /locus_tag="OO309_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5845563.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(47817..49244)
                     /locus_tag="OO309_000286"
     CDS             complement(47817..49244)
                     /locus_tag="OO309_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR5845564.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(49241..49948)
                     /locus_tag="OO309_000287"
     CDS             complement(49241..49948)
                     /locus_tag="OO309_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5845565.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            50112..51089
                     /locus_tag="OO309_000288"
     CDS             50112..51089
                     /locus_tag="OO309_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5845566.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(51159..51641)
                     /locus_tag="OO309_000289"
     CDS             complement(51159..51641)
                     /locus_tag="OO309_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR5845567.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            51876..54458
                     /gene="leuS"
                     /locus_tag="OO309_000290"
     CDS             51876..54458
                     /gene="leuS"
                     /locus_tag="OO309_000290"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR5845568.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            54473..55054
                     /gene="lptE"
                     /locus_tag="OO309_000291"
     CDS             54473..55054
                     /gene="lptE"
                     /locus_tag="OO309_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR5845569.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            55054..56085
                     /gene="holA"
                     /locus_tag="OO309_000292"
     CDS             55054..56085
                     /gene="holA"
                     /locus_tag="OO309_000292"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR5845570.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            56087..56728
                     /gene="nadD"
                     /locus_tag="OO309_000293"
     CDS             56087..56728
                     /gene="nadD"
                     /locus_tag="OO309_000293"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR5845571.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            56752..57363
                     /locus_tag="OO309_000294"
     CDS             56752..57363
                     /locus_tag="OO309_000294"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR5845572.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            57623..57940
                     /gene="rsfS"
                     /locus_tag="OO309_000295"
                     /gene_synonym="rsfA"
     CDS             57623..57940
                     /gene="rsfS"
                     /locus_tag="OO309_000295"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR5845573.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            57944..58411
                     /gene="rlmH"
                     /locus_tag="OO309_000296"
     CDS             57944..58411
                     /gene="rlmH"
                     /locus_tag="OO309_000296"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR5845574.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            58442..60343
                     /gene="mrdA"
                     /locus_tag="OO309_000297"
     CDS             58442..60343
                     /gene="mrdA"
                     /locus_tag="OO309_000297"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR5845575.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            60346..61458
                     /gene="mrdB"
                     /locus_tag="OO309_000298"
                     /gene_synonym="rodA"
     CDS             60346..61458
                     /gene="mrdB"
                     /locus_tag="OO309_000298"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR5845576.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            61469..62557
                     /gene="rlpA"
                     /locus_tag="OO309_000299"
     CDS             61469..62557
                     /gene="rlpA"
                     /locus_tag="OO309_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR5845577.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            62696..63907
                     /gene="dacA"
                     /locus_tag="OO309_000300"
     CDS             62696..63907
                     /gene="dacA"
                     /locus_tag="OO309_000300"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR5845578.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            64018..64281
                     /locus_tag="OO309_000301"
     CDS             64018..64281
                     /locus_tag="OO309_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR5845579.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            64382..65023
                     /gene="lipB"
                     /locus_tag="OO309_000302"
     CDS             64382..65023
                     /gene="lipB"
                     /locus_tag="OO309_000302"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR5845580.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            65282..66235
                     /locus_tag="OO309_000303"
     CDS             65282..66235
                     /locus_tag="OO309_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5845581.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            66444..67409
                     /gene="lipA"
                     /locus_tag="OO309_000304"
     CDS             66444..67409
                     /gene="lipA"
                     /locus_tag="OO309_000304"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR5845582.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(67510..67713)
                     /gene="tatE"
                     /locus_tag="OO309_000305"
     CDS             complement(67510..67713)
                     /gene="tatE"
                     /locus_tag="OO309_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR5845583.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(67842..68630)
                     /locus_tag="OO309_000306"
     CDS             complement(67842..68630)
                     /locus_tag="OO309_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR5845584.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            68723..69106
                     /gene="crcB"
                     /locus_tag="OO309_000307"
     CDS             68723..69106
                     /gene="crcB"
                     /locus_tag="OO309_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR5845585.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(69160..69369)
                     /gene="cspE"
                     /locus_tag="OO309_000308"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(69160..69369)
                     /gene="cspE"
                     /locus_tag="OO309_000308"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5845586.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(69544..70104)
                     /gene="pagP"
                     /locus_tag="OO309_000309"
     CDS             complement(69544..70104)
                     /gene="pagP"
                     /locus_tag="OO309_000309"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR5845587.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            70693..72078
                     /gene="dcuC"
                     /locus_tag="OO309_000310"
     CDS             70693..72078
                     /gene="dcuC"
                     /locus_tag="OO309_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5845588.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(72119..72460)
                     /locus_tag="OO309_000311"
                     /pseudo
     CDS             complement(72119..72460)
                     /locus_tag="OO309_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(72798..73307)
                     /locus_tag="OO309_000312"
     CDS             complement(72798..73307)
                     /locus_tag="OO309_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5845589.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            73470..73715
                     /locus_tag="OO309_000313"
     CDS             73470..73715
                     /locus_tag="OO309_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5845590.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            73690..75321
                     /locus_tag="OO309_000314"
                     /pseudo
     CDS             73690..75321
                     /locus_tag="OO309_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
BASE COUNT        18525 a        18953 c        19154 g        18728 t
ORIGIN      
        1 gctaaaacag tgatgaaatg aataatacga tggaaacgtc ttccatgtta attatcatgg
       61 aagacgttga tgttgaacac ctaaaccagc cccctgatac gaggctggct taagcgcgca
      121 ggcaatcagt aaaaatcacc ggttgccttt tcggcctgat ccatccacac cggtttatcg
      181 ctggttttcg accagacgcg gtgcaggtag ctgtaaaagc gggcgcggtc tttccagaaa
      241 agcatgaaag gcagcgccag aacgcccgct gcaaccgcat atgtacggcg taaaaagata
      301 agccatgcag gatattctct gtagagttcc atacttttct cccccttttg tatctacccg
      361 gtgaatagca ccggaaaaat gaatttgttt atctgatgaa aatagtaccg ccttttgtgt
      421 aattttacta ctcatccgac cacttatttt tgcttattga tggtttattt acattcacac
      481 tgtaattaaa ttacacaaaa gttaaattaa tactaaccat cagttaaatc atggcctttg
      541 ccatttttat acttttttta caccccgtcc gcagattttt gcgaaatctt tgcagccaga
      601 attctactct tccggtatca cttttaggcc actggaggtg cactgtgagt gcaggcgtga
      661 tagccggcgt gttgctggtg tttttattac tgggctattt ggtttatgcc ctgatcaatg
      721 cggaggcgtt ctgatggctg cgcaagggtt cttactgatt gccacgtttt tactggtatt
      781 aatggtgctg gcgcgtcctt taggcagcgg gctggcgcgg ctgattaatg acattcctct
      841 tccaggtaca gcgggcgttg aacgtatact ctttcgttta cctggcgtct ctgaccatga
      901 gatgaactgg aagcaatatc tttgtgccat tctcggcctg aacatgctgg ggctggcggt
      961 gctgtttttt atgttgctcg gtcagcacta tctgccgctt aatccacagc agttgccggg
     1021 gctgtcgtgg gatctggcgc tgaataccgc cgtcagcttt gtcacgaata ccaactggca
     1081 atcttatagc ggtgaaacca cgttgagcta tttcagccag atggcaggct taacggtaca
     1141 aaactttctt tctgccgcca gcgggattgc ggtgattttt gccttcatcc gtgcgtttac
     1201 tcgccagagc atgagcacac tcgggaatgc ctgggtcgat ctgctacgca tcacgttatg
     1261 ggtgctagtc cctgtggcgt tgttgatcgc actgtttttt attcaacaag gtgcgcagca
     1321 aaactttctg ccctatcagg cagtgaatac cgttgaagga gcgcaacagc tgttacccat
     1381 gggaccagta gcttctcagg aagcgatcaa gatgctcggt actaacggcg gtggcttctt
     1441 taatgccaac tcgtcgcatc cgtttgaaaa cccaaccgca ctgaccaact tcgtgcagat
     1501 gctggcgatc ttcttgatcc caacagcgct gtgctttgcc tttggtgaag tgacgggcga
     1561 tcgccgccag gggcgcatgt tgctgtgggc gatgtcagtg atttttgtca tctgcgtagg
     1621 cgtggttatg tgggcagaag ttcagggtaa tcctcatctg ctggcgctcg gcgcagacag
     1681 cagcatcaat atggaaggta aagagagccg tttcggcgtg ctggtcagta gcctgtttgc
     1741 ggtcgtgact acggcggcgt cctgtggcgc ggtgattgcg atgcatgatt cgtttaccgc
     1801 tctcggcggc atggtgccga tgtggctaat gcaaattggt gaagtggtgt tcggcggtgt
     1861 cggttctggt ctttacggca tgatgctgtt tgtcctgctg gcggtgttta ttgccgggct
     1921 gatgattggt cgtacaccgg aatatctggg taaaaaaata gacgtacgcg agatgaaact
     1981 gactgcactg gcgattctgg ttactccaac actggtactg atgggcgcgg cgttggcgat
     2041 gatgaccgac gccggacgta gcgccatgct taaccctagc ccgcatggtt ttagcgaagt
     2101 gctgtacgcc gtgtcgtccg ccgctaacaa caacggcagc gcctttgccg gattaagcgc
     2161 caactctccg ttctggaact gtttactggc gttctgcatg tttgtcggtc gcttcggggt
     2221 gattatcccg gtgatggcaa ttgccggttc gctggtgagt aaaaagagcc aacccgccag
     2281 ctccggcacg ctgccaacgc acggcccgct gtttgttggc ctgttaatcg gcaccgtgtt
     2341 gctggttggc gcactgacct ttatccctgc cctggcgctt ggtccggtgg cggaatatct
     2401 ctcctgatga tattgagtga gcactgaata tgagtcgtaa acaactggcg ctattcgaac
     2461 caacacttgt cgttcaggcg ctgaaagaag cggtgaaaaa attaaacccg caggcgcaat
     2521 ggcgcaatcc ggtgatgttt atcgtctgga tcggcagtct gctgaccacc tgtattagca
     2581 tcgcgatggc aagcggtgcg atgcctggca atgcgctgtt tagcgcggca attagcggct
     2641 ggctgtgggt caccgtactg ttcgctaatt tcgccgaggc gctggcagaa ggccgcagta
     2701 aagcgcaggc caacagtctt aaaggggtga aaaaaactgc ctttgcccgc aagctgcgtg
     2761 agccgaaata tggcgctgcg gcggacaaag ttcctgccga tcaactgcgt aaaggtgaca
     2821 tcgtgctggt agaagctggc gatattatcc cctgcgatgg tgaagtcatt gaaggcggcg
     2881 catcggtcga tgaaagcgcc atcaccgggg aatcggcacc ggtgatccgt gaatccggcg
     2941 gcgattttgc ctccgttacc ggcggcacgc gtattctttc tgactggctg gtgattgagt
     3001 gtagcgttaa ccccggtgag acatttctgg atcggatgat cgcgatggtg gaaggcgcac
     3061 agcgacgcaa aacaccgaac gagattgccc tgactatcct gctgattgct ctgaccatcg
     3121 tctttttact ggcaaccgcc acgctgtggc cgttttccgc gtggggcggt aatgccgtga
     3181 gtgtaacggt actggtggcg ctgctggtct gtctgatccc aaccactatt ggtggcctgt
     3241 tgtcagcgat cggcgtcgcc gggatgagtc ggatgctggg cgcgaatgtg attgccacca
     3301 gcggacgtgc agttgaagcg gcaggtgacg ttgacgttct gctactggat aaaaccggca
     3361 ccatcacact cggtaaccgt caggcgtcgg agtttatccc cgcgcagggc gtggatgaaa
     3421 aaacgctggc tgacgccgca caactggctt cgctggctga tgaaacgccg gaaggtcgca
     3481 gtattgtgat cctcgccaag caacgtttta acctgcgcga gcgcgatgtg cagtcgctcc
     3541 atgccacctt tgtaccgttt actgcgcaaa gccggatgag cgggatcaac atcgacaacc
     3601 gcatgatccg taaaggttct gtcgatgcca ttcgccgcca tattgaggct aacggcggtc
     3661 acttccctgc cgatgttgat caaaaagtcg atcaggttgc gcgtcaagga gccacgctgc
     3721 tggtggtggt ggaaggttct cgtgtgctgg gcgttattgc gctgaaagat atcgtcaaag
     3781 gcggtattaa agagcgcttc gcccagctgc gcaaatgggc attaaaacgg tgatgattac
     3841 cggcgataac cgtctgactg ccgccgcgat agctgcggaa gcgggtgtcg atgattttct
     3901 cgccgaagcg acaccggagg ccaagctggc attgatccgt cagtatcagg cggaaggtcg
     3961 tttggtagcg atgaccggcg acagcaccaa cgatgctacg gcgctggcgc aggcagatgt
     4021 cgcggtggcg atgaactccg gcacccaggc ggctaaagag gcgggcaata tggtggatct
     4081 cgactctaac ccgaccaagt tgatcgaggt ggtgcacatt ggcaaacaga tgctgatgac
     4141 ccgtggctcg ctgaccacct tcagcattgc caacgatgtg gcgaaatact tcgccattat
     4201 tccggcggcg ttcgcggcaa catatccgca gttaaatgcg ctgaacatca tgcgcctgca
     4261 ttcgcccgac tccgccattc tcagtgcggt cattttcaac gccttgatta tcgtcttttt
     4321 gattcctctg gcgttaaaag gcgtgagtta taaaccgctt accgcttctg ccatgttgcg
     4381 ccgtaactta tggatttacg gtctgggtgg gctgctggtg ccgtttattg gtatcaaagt
     4441 cattgattta ctgctaaccg tttgtagtct ggtgtgaggt ttaccatgag tggattacgt
     4501 ccggcattat cgacatttct ctttctgtta ttgattactg gcggcgttta cccgctgctg
     4561 accaccgcac tggggcaatg gtggtatccc tggcaggcca acggttcgtt gattcgtgaa
     4621 ggtgatacgg tgcgcggttc ggcattaatc gggcaaaatt ttaccggcaa cggctatttt
     4681 catggtcgcc cgtcggcaac agcagaaatg ccctataatc cacaggcttc tggcgggagc
     4741 aatctggcag tcagtaaccc ggagctggat aaacagatag ccgcacgcgt tgccgcatta
     4801 cgggccgcta acccggacgc cagcacgaac gttccggttg aactggtgac ggcgtcggca
     4861 agcggactgg acaataacat caccccgcaa gcggcggcct ggcagatccc acgcatcgca
     4921 aaagcgcgta atctcagcgt tgaacagctc acgcagctga tcgcaaaata cagccaacaa
     4981 ccgctggtga aatatatcgg ccagccggtc gtcaacattg tcgaactcaa tctggcactg
     5041 gataagcttg atgaataacg aacccttacg tcccgacccc gaccgtctgc tggaacaaac
     5101 tgccgcgccg catcggggga agctgaaagt tttcttcagt gcctgtgcag gcgtcgggaa
     5161 gacctgggcg atgctggcag aagcccagcg actgcgggcg caagggctgg atattgtggt
     5221 tggtgtggta gaaacccatg ggcggaaaga taccgccgcc atgcttgaag ggctggctgt
     5281 tctgcctctg aaacgccagg cgtaccgtgg gcggcatatc agcgagtttg atctcgatgc
     5341 cgccctcgcc cgccgtccgg cgctgatctt aatggacgaa ctggcgcaca gtaatgcgcc
     5401 aggttctcgt catcctaaac gctggcagga tatcgaagaa ctgctggaag ctggcattga
     5461 tgttttcaca accgtcaacg ttcagcatct ggaaagtctg aatgatgtgg tcagcggcgt
     5521 caccggaatt caggtacggg aaaccgtgcc cgatcctttt ttcgatgccg ccgacgacgt
     5581 ggtgctggtg gacttgcccc cggacgatct gcgccagcgg ctgaaagaag gcaaagtcta
     5641 tattgccggg caggcggagc gcgccattga acattttttc cgcaaaggca atctgatcgc
     5701 cctgcgcgaa ctggcactgc gccgtactgc cgatcgcgtt gatgagcaaa tgcgcgcctg
     5761 gcgggggcat cctggcgaag agaaagtctg gcacacgcgc gacgcgatcc ttttatgcat
     5821 cggccataac accggcagcg aaaactggtc cgcgcggcgg cgcggctgac gtcacggctg
     5881 gggagcgtct ggcacgcggt gtatgttgaa acccctgccc tgcatcgctt accggagaaa
     5941 aaacgtcggg caattctcag cgccttacgt ctggcgcagg agctgggcgc ggagacggca
     6001 acactttctg atccagcgga agagaaagcg gtagtgcgtt atgcccgtga acataatctc
     6061 ggcaagatta ttctcggtcg cccagcctcg cgccgctggt ggcgtcggga aacatttgct
     6121 gaccgactgg cgcgcatcgc ccccgatctc gatcaggtgc tggtcgcgct tgatgaacca
     6181 cccgcccgca cgattaacaa cgcgccggat agccgctcat ttaaagacaa gtggcgggta
     6241 cagattcagg gatgcgtggt tgccgccgcg ttatgcgccg ttatcacctt aattgccatg
     6301 cagtggctga tggcgtttga tgccgccaac ctggtgatgc tgtatctgct tggcgtggtg
     6361 gtggcgctat tttatggacg ctggccttcg gtggttgcca ccgtcattaa tgtggtgagt
     6421 ttcgatctct tttttatcgc cccacgcggc acgctcgccg tctctgatgt gcaatatctg
     6481 ctgaccttcg cggtgatgtt aaccgtcggg ctggtgatcg ggaaccttac tgctggtgtg
     6541 cgttatcagg cgcgggtagc ccgttaccgc gagcaacgca cacggcactt atatgaaatg
     6601 tcgaaagctc tggcggtggg ccgcagtccg caggacatcg ccgccaccag cgaacaattt
     6661 attgcctcca cgtttcatgc ccgcagtcag gtgttgttgc ccgatgacaa cggtaaattg
     6721 cagccgttaa cacatccgca aggaattacg ccatgggacg atgccatcgc gcagtggagt
     6781 tttgataaag gcctgcctgc gggcgcgggc accgacacgt tacccggtgt accgtaccag
     6841 attttgccgc taaaaagcgg cgagaaaacc tacgggctgg tggtggtgga gccggggaat
     6901 ctgcgccagt tgatgatccc ggaacagcag cgcctgctgg agacttttac gctgttagtc
     6961 gccaatgccc ttgagcggct gacgctaacc gccagcgaag aacaggcgcg gatggcaagc
     7021 gaacgtgaac agatccgcaa cgccctgctg gcagcgcttt cgcatgattt acgcacgccg
     7081 cttacggtgc tgtttggtca ggcagaaatc ttaacgctcg atctggcaag cgaaggatca
     7141 ccccacgccc gccaggccag cgagattcgt cagcatgtgc tgaacactac ccgactggtg
     7201 aataatctac tggatatggc gcgaattcag tccggcggct ttaatttgaa gaaagagtgg
     7261 ttaacgctgg aagaagtagt cggcagcgcg ctgcaaatgc cggaaccggg tttatcgtcg
     7321 cccatcaatc tttctctgcc agaaccgctg accttaatcc acgttgacgg gccactcttt
     7381 gaacgggtgc tgattaatct gctggagaac gcggtgaaat atgcgggtgc gcaggccgaa
     7441 attggtatcg atgcccacgt tgagggcgaa aatctacaac tggatgtctg ggataacggc
     7501 cccggttttc cgccaggcca ggagcagacg atatttgata agtttgctcg cgggaataaa
     7561 gagtcggcgg tgccgggtgt cgggcttggg ttggcaattt gtcgggcgat tgtggatgta
     7621 cacggcggca ccattaccgc gttcaaccga ccggaaggtg gtgcctgttt tcgtgttaca
     7681 cttccccagc aaaccgctcc tgaacttgaa gactttcatg aggatatgtg acaaacgttc
     7741 tgattgttga agatgaacag gctattcgtc gctttctgcg cacggcgctg gagggcgacg
     7801 ggatgcgcgt ctttgaggcc gaaactctgc aacgcggctt gctggaagcg gcaacccgta
     7861 agccagattt gattattctc gatctcggcc tgcccgatgg tgatgggatt gagtttatcc
     7921 gcgacctgcg ccagtggagc ccggtgccgg tgattgtgct ttccgcacgc agcgaagaga
     7981 gcgacaagat cgccgcgctg gatgccggag cggatgatta tctgagtaag ccgtttggta
     8041 ttggcgaatt gcaggcccgt ctgcgcgtcg ctttacgccg ccactccgcc actccgccac
     8101 cgccgcgccc gatccgctgg tgaaattttc cggtgttacc gtcgatttag ccgcccgcgt
     8161 gattcaccgg ggtgaggaag aggtgcatct cacaccaatt gagttccgcc tgctggcggt
     8221 gctgctcaac aatgccggaa aagtactcac ccagcgccag ctccttaacc aggtgtgggg
     8281 gccaaacgcg gtcgaacaca gtcactattt gcgtatttat atgggacatc tgcgacaaaa
     8341 actggaacag gatccggccc ggcctcgcca cttcattact gaaaccggta ttgggtatcg
     8401 gtttatgctt tgaattgtta ttcaataaat tcgcttaaat agtgattaat ttattattaa
     8461 acctgcgccg aatattatta tttggcaggt ttaatttctt aacgcaaata taaaacacga
     8521 aattacaaca tttaagtaac aaaacagcat ctaatatggc gatattcata attaatgatt
     8581 tattatctgg ggtatgatcg ttttttgttg atcttcttca cagattatag ccatttcatg
     8641 gatagaataa ctctaccttc aactgacaca gcaagcggta aaggtaaatg gaaaataata
     8701 accgcttaat gcctcatata aggcggacaa cccatatcat gaagtttacc catcgtaata
     8761 gttttgactt tcatttcttt aatgcccgct agtcttctga ctaaagggca ccccaacgta
     8821 caggtctccc tgactttaag cattacaggt taatacctgt attcctcggt gctcatatac
     8881 tgctaaccct tttaaactct aaataattcg agtcgcagca cttgcaactt gaggtatgac
     8941 gagtatagcc agttaccggg ctggtctggg ttattgcatc tgcaaaaagc aaactactga
     9001 tttatttatc agcggtggag ctttgctttt tttccgacgt gatcgatttc tcctttgaga
     9061 aattgaggac ctgctattac ctgaaataaa gagatgaata aaatgtcaga attaaaaatt
     9121 gccgttagcc gttcttgccc ggattgtttt tccactcatc gtgcatgcgt gaatattgac
     9181 gaaagtaatt atattgacgt tgccgccatt attttatcag tcaatgatgt tgaacgtgga
     9241 aaactcgata aaatagacgc tactggctat ggcattcctg tttttattgc aacggaaaat
     9301 gaagaacgtg taccagcaga atatctgcca cgtatttctg gtgtcttcga gcattgcgaa
     9361 tcacggaaag agttttatgg acgtcagtta gaaaccgctg ctagccatta tgaaacgcaa
     9421 ctgcgcccgc ccttcttccg cgcactggtc gattatgtca atcagggcaa tagcgctttt
     9481 gactgcccgg gtcatcaggg aggtgagttt ttccgtcgtc atccggcagg gaatcagttc
     9541 gtggagtact tcggagaaac gctgttccgt tccgacttat gcaacgcaga cgtagcaatg
     9601 ggcgatctgc tgattcacga aggcgcgcca tgcattgcac agcaacatgc ggcaaaagtg
     9661 tttaatgccg ataaaacata cttcgttcta aatggcactt catcttctaa caaagtggtt
     9721 ttaaacgccc tgctaacacc gggtgatctg gtgctgtttg atcgcaataa ccacaaatct
     9781 aaccaccacg gagcgttgct acaggctggt gcaacaccgg tttatctgga aacggcacgt
     9841 aacccgtatg gctttatcgg tggcattgat gcgcactgtt ttgaagaaag ttacctgcgt
     9901 gagctgatcg cggaagtcgc accgcagcgg gcaaaagagg ctcgtccttt ccgcctcgct
     9961 gtgattcagt taggcaccta cgacggtacg atttataacg cccgccaggt ggtggataaa
    10021 attggtcatc tgtgtgacta cattctgttt gactccgcat gggttggcta tgagcaattt
    10081 atcccgatga tggctgactg ttcgccgctg ttgctggatc ttaatgagaa cgatccgggt
    10141 attctagtta cgcaatccgt acataaacag caggctggtt tttctcaaac ttcacaaatc
    10201 cataaaaaag acagccacat caaagggcaa cagcgttatg taccgcacaa acgcatgaac
    10261 aacgccttta tgatgcacgc ctccaccagc ccgttctatc cgctgtttgc cgcactggat
    10321 atcaacgcca aaatgcatga aggtgtcagc ggtcgtaata tgtggatgga ttgtgtggta
    10381 aatggcatta atgcccgcaa actgatcctc gataactgtc agcatattcg tccgttcgta
    10441 cctgaactgg tggatggtaa accctggcag tcgtatgaaa cagcgcaaat tgcggttgat
    10501 ctgcgcttct tccagtttgt accaggggaa cactggcatt cttttgaagg ctatgcagag
    10561 aatcaatact ttgtcgatcc ttgcaaactg ttgctgacaa ccccaggtat tgatgcacgt
    10621 aacggcgaat atgaagcgtt cggtgtaccc gcgacgattc ttgctaactt cctgcgcgaa
    10681 aatggcgtag tgccggaaaa atgcgatctt aacgccatcc tcttcctgct gactccggca
    10741 gaagatatgg ccaaacttca gcaacttgtg ccctgctggt acgcttcgaa aaactgcttg
    10801 agtccgacgc gccattagca gaagtgctgc cttccatcta caaacagcat gaagagcgct
    10861 acgccggtta taccctgcgt cagttgtgcc aggaaatgca tgatttgtat gcccgccaca
    10921 acgtgaaaca actgcaaaaa gagatgttcc gcaaagcgca cttcccacac gtcagcatga
    10981 atccgcaaga agccaactac gcctatttac gcggtgaagt ggagctggtt cgtctaccgg
    11041 atgccgaagg tcgtatcgct gccgaagggg cgcttcctta tcctccgggt gtgctgtgtg
    11101 ttgttccggg tgaaatctgg ggtggtgctg ttttgcgtta cttcagcgct ctggaagaag
    11161 ggatcaacct gctgccaggt tttgcaccgg agctgcaggg tgtctatatc gaagaacatg
    11221 atggtcgtaa gcaagtttgg tgctatgtca tcaagcctcg tgatgcgcaa agcaccctgt
    11281 tgaaagggga aaaattatga gtcaggctaa atcgaacaaa atgggcgtcg ttcagttaac
    11341 catactgacg atggtcaaca tgatgggctc cggtatcatc atgctgccga caaagcttgc
    11401 cgaagttggg acaatttcaa ttatctcctg gctggtgaca gccgtaggct caatggcact
    11461 ggcatgggca ttcgcaaaat gcggtatgtt cagccgtaaa tcaggtggta tgggcggtta
    11521 tgccgagtat gctttcggta aatccggtaa ctttattgcg aactatacct acggcgtctc
    11581 actgctgatt gctaacgtcg cgattgctat ttcggcggtt ggttacggca ccgaactgct
    11641 cggcgcgagt ttgtcgccag tgcagattgg tcttgcgacc atcggggtgc tgtggatttg
    11701 taccgtggct aactttggtg gtgcgcgcat taccgggcaa atcagtagca ttaccgtgtg
    11761 ggggggcatt attccggtgg taggcctgtg cattatcggc tggttctggt ttagcccgac
    11821 gctttacgtt gattcctgga atccgcatca tgcaccgttc ttcagtgcag taggttcttc
    11881 catcgctatg acgctgtggg cttttcttgg tctggagtct gcgtgtgcga atactgatgt
    11941 agtggaaaac ccggaacgta atgtgccaat cgcggtactc ggcggtacgt taggtgcggc
    12001 ggtgatttat atcgtctcca ccaacgtgat tgcagggatt gtgcctaata tggagctggc
    12061 aaattcaacg gcaccatttg gcctggcctt cgcacagatg tttacaccag aagtgggtaa
    12121 agtcattatg gcgctgatgg tgatgtcctg ctgcggttcg ctgcttggct ggcagttcac
    12181 cattgcccag gtgtttaaat cttcatctga tgaaggctac ttccctaaaa ttttctcccg
    12241 tgtaaccaaa gtggatgcac cggtgcaggg aatgttgacc attgtgatta tccagagcgg
    12301 gttggcgctg atgacgatta gcccgtcgct aaacagtcag ttcaacgtgc tggttaacct
    12361 ggccgtggtg accaatatca ttccgtatat tctgtcaatg gcggcattag tcattattca
    12421 gaaggtcgct aatgtgcccc cctcaaaagc gaaagttgca aactttgttg cttttgttgg
    12481 cgcgatgtat agcttttatg cgctgtactc atccggggaa gaagccatgc tgtacggttc
    12541 catcgtgacc ttccttggtt ggacactgta tggtctggta tcaccacgct ttgaactgaa
    12601 aaataaacac ggttaaaaaa gcaaagtaaa aaagggcgat cttgcgaccg ctcttttttt
    12661 attaaatgtg tttacgcgtt tttcagaact tcgctaacaa tctcaaccgc ttctttctca
    12721 atctgcttgc gatgttcttc accgaggaag ctttcgcagt agatcttata tgcgtcttcc
    12781 gtgcctgacg gacgcgcggc gaaccagccg ttgtcagtca tcactttcag accgccaata
    12841 gaagcaccgt tgcccggagc agcagtcagg cgcgcggtga tcgggtcacc tgccagggtg
    12901 ctggcgctca ccatttccgg agacagctta gacagcgccg ctttttgtgc ggaagtcgca
    12961 gctgcctgca aacggttgta gctcggcgca ccaaagcgtt ttgccagttc gttgtagtgt
    13021 tcctgcgggt tcttaccggt gacagcggtg atttccgccg ccagcagaca cataatgatg
    13081 ccgtctttgt cggtggacca cggcgtgccg tcgaaacgca ggaaggaagc ccctgcgctc
    13141 tcttcgccgc caaagccgaa gctgccgtcg aacagaccat caacaaacca tttgaaacct
    13201 accggtactt ctaccagctt acggcccaag tcattgacca cacggtcgat catcgcagaa
    13261 gaaaccagcg ttttaccgac agcaacatct ttgccccact gcggacgatg ctggaacagg
    13321 tagttgatcg ccaccgccag gtagtggttc ggattcatca aacctgccgg agtgacgata
    13381 ccgtgacggt cataatccgg gtcgttagca aacgccagat cgaacttatc acgcagtgcc
    13441 agcagacccg ccatcgcaca ctcggaggag cagtccatac ggatcgcacc gtctttatcg
    13501 aggtgcataa agcggaaggt ttgatcgacc tgatcgttaa cgatggtcag gttgaggttg
    13561 taatactcgc caatgcgctt ccagtattcg ataccggaac cgcccaacgg atcaacgccc
    13621 agcgtcaggc ccgctttctg aatcgccgcc atatcaacga tatcggccag cccttccacg
    13681 aacggctgca ccagatcctg ctctttcaca tagccggatg ccatcgcttc gtcgagggag
    13741 atacgcttca cgcctttcag gccatcggcc atcagtgcgt tggccttgtc ttccaccact
    13801 ttggtgacgt tagtatcagc cgggccacca tttggcggat tgtacttgat accaccatct
    13861 tccggcgggt tatgggacgg tgtaatcacg ataccgtctg ccagcgggcc acctttttta
    13921 ttgtgaacca ggatggcatt ggaaacggca ggcgtcggag tgaagccatt gttttcctgc
    13981 acaatgacat caacgccgtt cgctgccagc acttccagta cggaaatgaa cgcaggctcg
    14041 gacagggcgt gagtatcttt acccacatag caagggccag tgatgccgtt tttcgcacgt
    14101 tcttcagcaa ttgcctgagc gatcgccaga atgtgcggct cgttaaagct gtggcgcgct
    14161 gcactgccac ggtgaccgga agtaccgaat ttcaccgcgt gctccgcatt ccctgcttct
    14221 ggtttcagta cataatattg cgccgtcagt tgggcgacgt taatcaaatc actctgttgt
    14281 gcaggttggc ctgcacgatt gtggattgcc attgctttgt cctttgtctg caacgtttta
    14341 gatagttccg caaaccttct caatcaattc cgccgggaat tgcatcgact gcatgatgtg
    14401 ttcgatcatg ctgcatttac ggccggtgtt ggtgttggtg atcacccaat acggcgtgcc
    14461 tggcacatgt ttcggcttgg tctgattacc atttttcagc agcgtttgtt catctgccgc
    14521 aaagtacacg cgtgtacgac cgtgcaacga ttccgttgct tcggcaaacg cctgggcgtc
    14581 aagagaatat agtgtagaca acagcagcat aaagcgattg accgctcgct tttgctctgc
    14641 gtattcgtcc gaaagcagaa gttcacgcat tgcgcgaacc ttgtctttaa tcgttttgac
    14701 cggcttcgct tcgacgatag caggtgacgc aacgcgaacc tctttcgtca ccggagcagc
    14761 aggctgtgat gcggcggaaa atttcaacat acgccgtaaa atgtcggatg cgctctcgcc
    14821 gatatgctta gtgtggctgg caatatagct gtagagttca tcatcaactt caatcgtttt
    14881 catcttaatc cagtgcagtg tcttagctga atacaagtcg ccaagagtat atgaataaat
    14941 cccggcagcg gatagcgtca agcctggctt gcagaaaaac tcggaataaa ccttatttgt
    15001 tgcagccctg tggcacaatg gtcaacataa taccctaacc cgacaacgcg aaaaaaagaa
    15061 ctttgccatg aaattaaata tccgcgcgca aactgcacaa aaccagcaca ataattctcc
    15121 catcgttctt gtccatggtc tgtttggtag cctcgacaac cttggcgtac tggctcgcga
    15181 tctggtaaac gatcacaata tcatccaggt tgatatgcgt aaccacggtc tttcaccgag
    15241 agagccggta atgaattacc cggcgatggc gcaggatctt gttgataccc tggatgcact
    15301 gcagatcgac aaagcaacat ttatcggtca ctccatgggc ggtaaagcgg taatggcact
    15361 tactgcactg gcccccgatc gcatcgataa actggtggcg atcgatatcg caccggtcga
    15421 ctatcacgta cgccgtcatg atgagatttt cgcagctatc aacgcggtca gtgaatcgga
    15481 cgcacaaact cgccagcaag cagcagcaat aatgcgccag catcttaatg aagaaggggt
    15541 gattcagttt ctgttgaaat cttttgttga cggggagtgg cgctttaacg tgccggtatt
    15601 gtgggatcag tatccgcata ttgtgggttg ggagaaaatc ccggcatggg atcaccctgc
    15661 cctgtttatc cctggcggca attctccgta tgttagcgag cagtaccgtg atgatttact
    15721 ggcacaattt ccacaggcac gggcgcatgt gattgcaggc gcgggtcact gggtccatgc
    15781 tgaaaaaccg gatgcggtat tacgcgctat ccgtcgctat ctcaatgatt aacgcaactg
    15841 attaaaaacc cagcgtccgc gtcgctttcc gcgcgtgggc gttggcgtga ctcatcgcgc
    15901 tgatgtatta tggcgcgcta tccatccggg cagaggcctg gctgattgtt tcccccgaag
    15961 tcaccaagat catggccaaa gaacaaacgg accgtacgac attagatctg ttcgcgcacg
    16021 agcgtcgacc gggacgaccg aaaactaatc cgctttcgcg cgatgaacag ctgcgtatta
    16081 ataaacgcaa ccagctaaaa cgcgacaaag tacgtggcct taagcgtgtc gaactgaagc
    16141 tgaacgcgga agctgtcgag gcgctgaacg agctggcgga atcgcgtaat atgagccgta
    16201 gcgaactgat cgaagagatg ttaatgcagc aactggcggc gctgcgtagt cagggcatcg
    16261 tttaaatttc cactttcatg tagcacagtg tgcagtcctg ctcgtttgct gattccgcaa
    16321 gactgcctgt tctgctatga ttgcctttat ccgtgggcaa ttttccaccc ccatttcaat
    16381 aagtttcaag aggttatttc actcatggct atcactggca tctttttcgg cagcgacacc
    16441 ggtaataccg aaaatatcgc aaaaatgatt caaaaacagc ttggtaaaga cgttgccgat
    16501 gtccatgaca ttgcaaaaag cagcaaagaa gatctggaag cttacgacat tctgctgctg
    16561 ggcatcccaa cctggtatta cggcgaagcg cagtgtgact gggatgactt cttcccgact
    16621 ctcgaagaga ttgatttcaa tggtaaactg gttgcgctgt ttggttgtgg tgaccaggaa
    16681 gattacgccg aatatttctg cgacgcattg ggcaccatcc gcgacatcat tgaaccgcgc
    16741 ggtgcaacca tcgttggtca ctggccaact gcgggctatc atttcgaagc atcaaaaggt
    16801 ctggcagatg acgaccactt tgtcggtctg gctatcgacg aagaccgtca gccggaactg
    16861 accgctgaac gtgtagaaaa atgggttaaa cagatttctg aagagttgca tctcgacgaa
    16921 attcttaatg cctgatgtga tgcggcgtag actcatgtct acgccgtatt aatagataat
    16981 gccaatcaaa ataattgcta caaatttgta acttttgctg ttgtacctat acaatgtccc
    17041 agtgttcaag tggccttgcc gttgtaaatg taagctgtgc cacgttttta ttaacaatat
    17101 ttgccaggga cttgtggttt tcatttaggc gtggcaattc tataatgata cgcattatct
    17161 caagagcaaa ttctgtcact tcttctaatg aagtgaaccg cttagtaaca ggacagattc
    17221 cgcatgactg ataacaatac cgccctaaag aaagctggcc tgaaagtaac gcttcctcgt
    17281 ttaaaaatcc tggaagttct tcaggagccg gacaaccatc acgtcagtgc ggaagattta
    17341 tacaaacgtc tgatcgatat gggtgaagaa attggtctgg ctacggtata tcgcgtactg
    17401 aaccagtttg acgacgctgg tatcgtcacc cgccacaatt ttgaaggcgg taaatccgta
    17461 tttgaactga cacagcaaca tcaccacgat cacctgattt gcctcgactg cggcaaggtt
    17521 atcgaattta gtgatgattc catcgaagcg cgtcagcgtg aaattgccgc aaaacatggc
    17581 attcgcctga ctaaccacag tctctatctt tacggtcact gtgccgaagg cgattgccgc
    17641 gaagatgagc acgcgcacga aggcaaataa gccagcctga acgagaaaag ccaacctgcg
    17701 ggttggcttt tttatacaag ggaaataaga aaagcgagag ttacagctct cacttatttg
    17761 ttattactgc gtatttcctg ccagacctta tcacaatcgg ctttcaccgc ctgatcattt
    17821 ccggtttgcg ttgcacgcag acactgctga taatccagta cgcgtacact ttcctggtcg
    17881 gcaaactgct gatgttgttt ctctttcttc agcacgttta acacgctctg gcaggcttca
    17941 attttttctg gcgaaccttg cgcggtgttg atacaggcgc tgtatgcctc tttcagacgg
    18001 ctgtcttcct gtggtgcggt tgattgcgca caagctacca gccccgatgc catcatggcg
    18061 atgagaatca gttttttcat tgaatgcggc aggcacaaaa cctgccgcgt cgggcatcag
    18121 aagatggtga atggtgcgat taccataaat ttcacgtcac gctcatcctg gaagatgtta
    18181 ccgtaaccgc cgccccagct tgggatgtcg gagtggttgt cgtattcggt gaagtgcagt
    18241 ttgaacatcg tgcctttggc gcgaccgtcc tgaatggtgt agaccgcatc caggctgtag
    18301 gcagactctt caatagtccg gtttttgtcg tagtacgcat ccggattgct ctgccaggtc
    18361 gcaggtttag catcccatgc gtaaacgtag gaagcgccga tggcgaagcc tggaagattc
    18421 cagtttttca ggtcatacat cgcactgaag aagaccgctt tttcgccgtt agcgttgaag
    18481 tcggaacggt tatcccacca gatatccagg cggccgttgg aggaagcgta ggttggggtc
    18541 atacgttgca ggaagtatcc ctgctgactg tcagccttaa tccaggtgcc ttcgaggcgc
    18601 aaatccacta cgtcagccgc ccggtaacca aaggtcaacg cttgcagcca ggcggtgccg
    18661 tcataaagat cgttgacgct gcgatcgtca actttatcgc gggtaccata gaactggtag
    18721 ctggtggtta acgggctacc ggcgatatca aatttgtagc tggctttggc aaaatattga
    18781 tcgatatacc cttccgcctg accaaatgcc gcttccagta cgaagttatt tttgaagtcg
    18841 tatttcgcac cgatggagtg cagataatca actttggtgg ttttatcgtt ctgataaaac
    18901 tcatccattt ccagatgcca cggcgctttg tattcgttgg tccacatgta ggagaaactc
    18961 aaggcaccag catcgccgta atcaaaattc gccccggctt ccgccccctg ataagtaccc
    19021 ggcataaagc tccagtgcgg cgctaacagc gtttgaccgg ttggctgaat gtaacctgcc
    19081 ctcgcccaaa ccggaccgta tttaaatttg gccgcagctt tatacaggct tataccgctt
    19141 ttgtcgccgg accagtcttc gtcataggct ttattacttt ttgaaaacgc gatttcgttc
    19201 gggtgggagc tgtcgccgtt ttccgccatt tcaatcgccg taaacgcagc aatatcaagg
    19261 ccgaacatat cagcagcata accggactga aaatcgaggt tggcattcca ggtggagtga
    19321 gaaaggttgg ttttgtattt gtcgccgtcg gtaacatctt tacggtcgcg ttcacgctgc
    19381 cagtaataga taccgccggt taaggttgaa tcgtcgatga atccttcggc gcgagcctcc
    19441 ggcattgcca taaagcccga cattgctgta acaccggcaa tagccagcgc cagcgtacta
    19501 cgtttgccac taaacgtacg catgggttaa tcctctttga cgtataaatt gctgcaccaa
    19561 aggtgaacag cgaaaaaaaa taaaaattaa aaaagtagcg ttgagaaaag tctcgctgac
    19621 tacaggaaca gactattttt cgcgacgcga attatcaatc tctttctgta accagaatgt
    19681 aagaacatga gcaaccgcac atattttgat gatttttgtt acaaggctta agtttgcggt
    19741 tgaaaggctt gcagaagaat aaaaacgtga aattccagca agatgaaagc ggttacattt
    19801 tatttaatta tgttttgtga ttttaaattt attttatgga taaattgcga agcggaatta
    19861 attcgcgaag aaaagcagaa aaaacgccgc agagcgcggc gtttgggatt gtttgctatt
    19921 tcttgccgga tgcggcgtga acgccttatc cggcctacaa aagcacgcaa attcaatata
    19981 ttgcagagat catgtaggcc tgataagcga agcgcatcag gcaatttagc gtttgaaatt
    20041 tattgtctat ttattgccgg atgcgacgta aatgcctcac ccggcttggt agaacttaaa
    20101 attactcgcc tactttcgcc caggtatcac gcagcccaac ggtgcggtta aataccggtt
    20161 tttcggccgt ggaatggcgg ctgtcgaggc agaagtaacc ttcacgctca aactggaatg
    20221 ctttacccgc aaccgcatct ttcagcgacg gttcagcaaa gccctgtttg atcaccagcg
    20281 attccgggtt aatcaccgac aggaaatcat ccgcagcacc tgggttaggc acgctgaaca
    20341 gacgatcata caaacggatt tcaaccggca gcgcatgtgc cgcgctcacc cagtgaataa
    20401 cacctttgac tttacgacca tctgccggat ctttgcttaa ggtgtcggcg tcataagtac
    20461 agaagatggt ggtgatatta ccttcggcat ctttctccac gcgttcagcc ttaatgacgt
    20521 aagcattacg cagacgcact tctttaccca gcaccagacg tttgtactgc ttgttagctt
    20581 cttcgcggaa atcggcgcga tcgatccaaa tttcaccgct aaacggcacc tgacggctcc
    20641 ccatttccgg tttgttcgga tggttcggca tggtgaccat ttcgccttcg ccctgatagt
    20701 tttcgataac cagtttcacc ggatcgataa ccgccattgc gcgcggcgca ttttcgttga
    20761 gatcttcacg gatgcaggat tccagcgacg ccatctcaat ggtgttgtcc tgcttggtca
    20821 cgccgatgcg tttgcagaac tcacgaatag aagccgcagt gtaaccacga cgacgcagac
    20881 cggaaatggt cggcatacgc gggtcatccc agccttcaac gtgcttgtcg gtcaccagca
    20941 ggttcagctt acgcttggac atcacagtgt attccagatt caggcgcgag aattcgtact
    21001 ggcgcggatg aacaggaatg gtgatgttat ccagtaccca gtcgtacaga cgacggttgt
    21061 cctggaactc aagcgtacac agagagtgcg taataccttc cagcgcatcg ctgatgcaat
    21121 gggtgaagtc gtacatcggg tagatgcacc acttgttgcc agtctggtgg tgttcagcaa
    21181 atttaatgcg gtacagcacc ggatcgcgca ttacgataaa tggcgacgcc atgtcgattt
    21241 tcgcacgcag gcaggcttta ccttcttcaa aaccaccggc acgcattttt tcgaacagcg
    21301 ccaagttctc ttcaacgctg cggtcgcggt acgggctgtt tttacccggc tgcgtcaggg
    21361 taccgcggta ttcgcggatc tgttccggtg tcagttcatc aacgtacgcc aggcctttat
    21421 tgatcagttc gatcgcatag gcgtggagct gatcaaaata atcggaggag taacggacgt
    21481 taccagacca gtgaaaacct aaccactcta cgtcgttttt gatcgaatcg acatactcga
    21541 tatcttcttt taccgggtta gtgtcgtcga aacgcaggtt gcactggcct ttatagtcct
    21601 gggcgatccc gaagttcagg cagatagatt tcgcatggcc aatatgcaga tagccattcg
    21661 gctccggcgg gaaacgggtg tgtactgtga tgtgcttacc actggccaga tcttcatcga
    21721 tgatctgacg gataaagtta gtcgggcggg cttctgcctc actcatcgtg gattcctcaa
    21781 agcgtaaaca acgtataacg gcgtatgatc ttataagcgg gacaggctga caactgttag
    21841 ttttttaaaa ttgataatct aacaaggatt atggggatga ttgatgaaaa aaaaagcggc
    21901 agagaagatc tccaccgctt gtcattgttg gatgcgacgc tgccgtgtcg catcaggcat
    21961 aaagcagatt actttttgat ttcatacagc ggtgtttgac ccgccacaac atggccctga
    22021 gctttaatga gcaagccact gaaatcgtcg atattgctgc aaaccaccgg gctaatcatc
    22081 gagcgggcgt tagcgttcag gtaatccaga tccatttcca gaatcggttg ccctgcgctt
    22141 acctgcgcac cctcttccac cagacgttta aagcctttac cttccagcgc tacggtgtcg
    22201 atacccatat ggacgacgat ctccgcgcct ttttcggttt ccaggcagaa cgcgtggttg
    22261 gtgttgaaga ttttcacgat tgtccctgcg gctggtgata cgacgatttt atctgtcggt
    22321 ttcaccgcca caccgtcacc caccgctttg ctggcgaatg cttcgtcagg aacctgatcc
    22381 agtgccacga catcaccggt aatcggcgat accagctccg cgatagatac cgcgtttggt
    22441 acagcctgcg gttttgctac aggcgcggca gttgccggag ttgcttcagc tgacgcagcg
    22501 gctaccggac cacgggcaac gactttcttc atcgcatcgc cgatggattc tgctttcgcg
    22561 ccaacaatca cctgaatagt ctgtttgttc agtttcacta caccagaagc acccagacgt
    22621 ttacacatcg tatcgttgac gcgggcagag tcagccacgg tcaggcgcag acgggtaata
    22681 caggcatcaa tcgctttcag gttgtcagta ccgccaaccg cagcaatata gttggttgcc
    22741 agttgagtca gaccttcttc agtattgctg ttggcttctt cagtaacgat ctcgtcttct
    22801 ttatcttcac gacccggcgt tttcaggttg aacatgcgga taaccaaact gaacaccacg
    22861 aagtagatag cgaagaagat aacgcccatc accagcagca tccagacgtt ctggctggcg
    22921 gcaggcaggt tatacatcaa cgcgtagtcg atagcacccg cagagaaaga gaagccggca
    22981 tggataccca gcagcgttgc cacaaacagg ctgataccgg tcagcagtgc gtgcaggagg
    23041 tacagcagcg gcgcaaggaa catgaacagg aattccagcg gctcagtcac accggtcagg
    23101 aacgcagtaa cagcaacaga aagcagcatc ccgccaacca tcggacgacg ctctttcggt
    23161 gctgcgaagt acatcgccag cgccgcaccc ggcagaccga acatcatgat cgggaagaag
    23221 ccggacatga acatccccgc ggtgccgtca ccagcgtaga aacggttgat gtcgccgtgg
    23281 aaaaccgtac ccgccgcgtt ggtgaattca ccaatctgga accaggcgat ggtgttcagc
    23341 acctgatgca gaccggttgg gatcagcaga cggttgatga aaccaaagat accggaaccc
    23401 agcgcgcccg cagaaacgat ccactcgccg cctgcatgga tagcgtgctg taccggcggc
    23461 cagacgtaac caaaaatggc cgccagcacc agacagaaga agccggtggc gatcggcaca
    23521 aagcgtttgc cgccgaagaa gctcaggaag tccggcagtt taatatcgga ccaacggtta
    23581 taggctgcgc caccaaccag accggtaatg atacccgcca gtacacccat gttaatttct
    23641 gggttgatgg tcaccatcgc tttggttaac acaaagtaac ctaccgcacc cgccagcgcc
    23701 gccgcacctg cgctgtcttt cgaccagctg gatgccacac cgatggcgaa gattaatgcg
    23761 aggttatcaa aaattgcacc gcccgcctgg gcaataaacg caacgttaag taaatctggc
    23821 tgaccgaatc gcagcaacag cgccgccacc ggcagcaccg cgatagggag ctgtaacgcc
    23881 ctaccgagtc gctggaaaaa acctaaaata ttcatcttat tccccctacg agaaccctat
    23941 ttggctcgtt taaagccgta tttttatttt gctgcaaatt ttactgccga tgttctgtaa
    24001 tcagattgtt agatcatctg ctacagagtg tgtgaaaatt taattcgcat cgcaaattaa
    24061 acgcgtgtct tttgtgagtt ttgtcaccaa atatcgttat catcactccc ttttactggc
    24121 taaaccagaa aacttatttt atcattcaaa aaatcaagtc ggattgacgc ctgtctgcgc
    24181 aaatccaggt tacgcttaaa gatgtctaat ccgccaacgg cttacatttt acttattgag
    24241 gtgaataatg agactgatcc ccctgactac cgctgaacag gtcggcaaat gggctgctcg
    24301 ccatatcgtc aatcgtatca atgcgttcaa accaactgcc gatcgtccgt ttgtactggg
    24361 cctgccgact ggcggcacgc cgatgaccac ctataaagcg ttagtcgaaa tgcataaagc
    24421 aggccaggtc agctttaagc acgttgtcac cttcaacatg gacgaatatg tcggtctgcc
    24481 gaaagagcat ccggaaagct actacagctt tatgcaccgt aatttcttcg atcacgttga
    24541 tattccagca gaaaacatca accttctcaa cggcaatgcc ccggatatcg atgccgagtg
    24601 ccgccagtat gaagaaaaaa tccgttctta cggaaaaatt catctgttta tgggcggtgt
    24661 aggtaacgac ggtcatattg catttaacga accggcgtct tctctggctt ctcgtactcg
    24721 tatcaaaacc ctgactcatg acactcgcgt cgcgaactct cgtttctttg ataacgatgt
    24781 caatcaggtg ccaaaatatg ccctgactgt cggtgttggt acactgctgg atgccgaaga
    24841 agtgatgatt atggtgctgg gtagccagaa agcactggcg ctgcaggccg ccgttgaagg
    24901 ttgcgtgaac catatgtgga ccatcagctg tctgcaactg catccgaaag cgatcatggt
    24961 gtgcgatgaa ccttccacca tggagctgaa agttaagact ttaagatatt tcaatgaatt
    25021 agaagcagaa aatatcaaag gtctgtaatt gttatccctg ccctggttcc ttgctcaggg
    25081 caatattttt taaaatcggg gggcagaatg tatgcattaa cccagggccg gatctttacc
    25141 ggccacgaat ttcttgatga ccacgcggtt gttatcgctg atggcctgat taaaagcgtc
    25201 tgtccggtag cggaactgcc gccagagatc gaacaacgtt cactgaacgg ggccattctc
    25261 tcccccggtt ttatcgatgt gcagttaaac ggctgcggcg gcgtacagtt taacgacacc
    25321 gctgaagcgg tcagcgtgga aacgctggaa atcatgcaga aagccaatga gaaatcaggc
    25381 tgtactaact atctgccgac gcttatcacc accagcgatg agctgatgaa acagggcgtg
    25441 cgcgttatgc gtgagtacct ggcaaaacat ccgaatcagg cgttaggtct gcatctggaa
    25501 ggtccgtggc tgaatctggt aaaaaaaggc acccataatc cgaattttgt gcgtaagcct
    25561 gatgccgcgc tggtcgattt cctgtgtgaa aacgccgacg tcattaccaa agtgaccctg
    25621 gcaccggaaa tggttcctgc ggaagtcatc agcaaactgg caaatgccgg gattgtggtt
    25681 tctgccggtc actccaacgc gacgttgaaa gaagcaaaag ccggtttccg cgcggggatt
    25741 acctttgcca cccatctgta caacgcgatg ccgtatatta ccggtcgtga accgggcctg
    25801 gcgggcgcga tcctcgacga agctgacatt tattgcggta ttatcgctga tggcctgcat
    25861 gttgattacg ccaacattcg taacgctaaa cgccttaaag gcgacaaact gtgtctggtt
    25921 accgacgcca ccgcgccagc aggtgccaac attgaacagt tcatttttgc gggtaaaaca
    25981 atatactacc gtaacggact ttgtgtggat gagaacggta cgttaagcgg ttcatcctta
    26041 accatgattg aaggcgtgcg taatctggtc gaacattgcg gtatcgcact ggatgaagtg
    26101 ctgcgtatgg cgacgctcta tccggcgcgt gcgattgacg ttgaaaaacg tctcggcacg
    26161 ctcgccgcag gtaaagtagc caacctgacc gcattcacac ctgattttaa aatcaccaag
    26221 accatcgtta acggtaacga ggtcgtaact caataagaga aagtatgaca ccaggcggac
    26281 aagctcagat aggtaatgtt gatctcgtaa aacagcttaa cagcgcggcg gtttatcgcc
    26341 tgattgacca gtacgggcca atctcgcgga ttcagattgc cgagcaaagc cagcttgccc
    26401 ccgccagcgt aaccaaaatt acgcgtcagc ttatcgaacg cgggctgatc aaagaagttg
    26461 atcagcaggc ctccaccggg ggccgccgcg ctatctccat cgtcaccgaa acccgcaatt
    26521 tccacgcaat cggcgtacgg cttggtcgtc atgacgccac catcactctg tttgatctca
    26581 gcagcaaagt gctggcagaa gaacattacc cgctgccgga acgtacccag cagacgctgg
    26641 aacatgccct gctgaatgcc attgctcagt ttattgatag ctaccagcgc aagctgcgcg
    26701 agctaatcgc catttcggtg atcctgccag ggcttgttga cccggacagc ggcaaaattc
    26761 attacatgcc gcatattcag gtagaaaact gggggctggt agaagcactg gaagaacgtt
    26821 ttaaagtgac ctgtttcgtt ggtcacgata tccgtagtct ggcgctggcg gagcactact
    26881 tcggtgcaag tcaggattgc gaagactcca ttctggtgcg tgtccatcgc ggaaccgggg
    26941 ccgggattat ctctaacggg cgcattttta ttggccgcaa cggtaacgtc ggtgaaattg
    27001 gccatattca ggtcgaaccg ctgggtgaac gctgccactg cggcaacttt ggctgcctgg
    27061 aaactatcgc cgccaacgct gccattgaac aacgggtgtt gaatctgtta aagcagggct
    27121 accagagccg tgtgccactg gacgactgca ccatcaaaac tatctgcaaa gccgcgaaca
    27181 aaggcgatag cctggcctcg gaagtgattg agtatgtcgg tcgtcatctg ggtaaaacca
    27241 tcgccattgc tatcaactta tttaatccgc aaaaaattgt tattgctggt gaaatcaccg
    27301 aagccgataa agtgctgctc cctgctattg aaagctgcat taatacccaa gcgctgaagg
    27361 cgtttcgtac taatctgccg gtggtacgtt ctgagctgga tcaccgctcg gcaatcggcg
    27421 cttttgcgct ggtaaaacgc gccatgctca acggtatttt gctccagcat ttgctggaaa
    27481 attaatgtgc ttttatagtg gcgcttattg ttgtcaatat tctgggtagt ccatgaccat
    27541 taaaaatgta atttgcgata tcgacggcgt gctgatgcac gataacgtcg ccgtaccggg
    27601 tgcagcggaa tttttgcacg ggattatgga taaaggcctg ccgctggtgt tgctgaccaa
    27661 ctatccttcg cagactgggc aagatctggc gaaccgcttt gccactgccg gtgtcgatgt
    27721 gccggatagc gtgttttata cctctgcgat ggcgaccgcc gatttcctgc gtcgctagga
    27781 aggcaagaaa gcgtatgtgg tgggcgaagg cgcactgatt catgaactgt acaaagccgg
    27841 tttcactatt accgatgtga accctgattt cgtgattgtt ggcgaaacac gttcctacaa
    27901 ctgggacatg attcataaag cagcctattt cgtcgctaac ggtgcacgct ttatcgccac
    27961 caacccggac acccacgggc gcggttttta tcccgcttgt ggcgcgttgt gtgcagggat
    28021 tgagaaaatc tccgggcgca aaccgttcta tgttggtaag cccagcccgt ggatcatccg
    28081 cgcggcatta aacaaaatgc aggcgcattc ggaagaaaca gtgattgtcg gcgataacct
    28141 gcgtaccgat attctggcgg gcttccaggc cggtctggag acgattctgg tgctttctgg
    28201 tgtttcatcg ctcgacgata tcgacagtat gccgttccgc ccaagctgga tttacccgtc
    28261 agtcgctgaa atcgacgtta tctgaatact acccacgtct gcggacgtgg ttggctctga
    28321 tgacaaacgc caaactgcct gatgcgctac gcttatcagg cctacgcagc tcctgcaata
    28381 tattgaattt tcatgctttt gtaggccgga taaggcgttc acgccgcatc cggcatgaac
    28441 aaatcgcact ttatcagcaa tttggccccc gtaaaatata accattcaat aaaacccgct
    28501 aaaaattatc gattcatcaa taaatacatc ctcacaccag accctttttc accaatcgac
    28561 aatcaccatt acgtttttta tttttgcact gccaaatagc ttgcgcacag tgctgctttg
    28621 cggcattttt ttaaacaagc aaacacaaca agcaacaaat accaggttaa cggagaaggt
    28681 tatgtgttca atttttggcg tattcgatat caaaacagac gcagttgagc tgcgtaagaa
    28741 agcactcgag ctgtcacgcc tgatgcgtca tcgtggcccg gactggtccg gtatttatgc
    28801 cagcgataac gccattctcg ctcacgaacg tctgtcaatt gttgacgtta acgcgggggc
    28861 gcaacctctc tacaaccaac aaaaaaacca cgtactggcg gtaaacggtg aaatctacaa
    28921 ccaccaggca ttgcgcgctg aatatggcga tcgttatcag ttccagacag ggtctgactg
    28981 tgaagtgatc ctcgcgctgt atcaggaaaa agggccggaa tttctcgacg acttgcaggg
    29041 catgtttgcc tttgccctgt acgacagcga aaaagatgcc tacctgattg gtcgcgacca
    29101 tctggggatc atcccactgt atatggggta tgacgaacac ggtcagctgt atgtggcctc
    29161 agaaatgaaa tcgctggtgc cagtttgccg cacgattaaa gagttcccgg cggggagcta
    29221 tttgtggagt caggacggcg aaatccgttc ttattatcat cgcgactggt tcgactttga
    29281 tgcagtgaaa gataacgtga ccgacaaaaa cgagctgcgt caggcactgg aagattccgt
    29341 taaaagccat ctgatgtctg atgtgcctta cggtgtgcta ctttctggtg gtctggattc
    29401 ctcaattatt tccgctatca ccaagaaata cgcagcccgt cgcgtggaag atcaggaacg
    29461 ctctgaagcc tggtggccgc agttacactc ctttgctgta ggtctgccgg gttcaccgga
    29521 tcttaaagca gcccaggaag tggcaaacca tctgggcacg gtgcatcacg aaattcactt
    29581 cactgtacag gaaggtctgg atgccatccg cgacgtgatt taccacatcg aaacttatga
    29641 tgtgaccact attcgcgctt caacaccgat gtatttaatg tcgcgtaaga tcaaggcgat
    29701 gggcattaaa atggtgctgt ccggtgaagg ttctgatgaa gtgtttggcg gttatcttta
    29761 cttccataaa gcgcccaacg ttaaagaact gcatgaagag acggtgcgta aactgctggc
    29821 cttgcatatg tatgactgcg cgcgcgccaa caaagcgatg tcagcctggg gcgtggaagc
    29881 acgcgttccg ttcctcgaca aaaaattcct tgatgtggcg atgcgcatta acccgcagga
    29941 taaaatgtgc ggtaacggca aaatggaaaa acacatcctg cgtgaatgtt ttgagtcata
    30001 cctgcccgca agcgtggcct ggcggcagaa agagcagttc tccgatggcg tcggttacag
    30061 ttggatcgac accctgaaag aagtggcggc gcagcaggtt tctgatcagc aactggaaac
    30121 tgcccgcttc cgcttcccgt acaacacgcc gacctcaaaa gaagcgtatc tgtaccgcga
    30181 gatctttgaa gaactgttcc cacttccgag tgccgctgag tgcgtgccgg gcggtccttc
    30241 cgtcgcgtgt tcttcagcta aagcgatcga atgggatgaa gcgttcaaaa aaatggacga
    30301 tccgtctggt cgtgcggttg gcgttcacca gtcggcgtat aagtaagaaa tgcggtactg
    30361 ccccggagaa atccggggct gttttagtta ttgcgctgct tgttgattct gatatgcagt
    30421 aaggaaatgt tccgggtttg ccacatcact gtttgacaac tcatcactgt aggccagtat
    30481 ttcatagatt aatttcccac cactattgtt gaattccgaa tttcatcctg tacgacaaaa
    30541 gaatggctgt agggtgggga aaaacacaaa gtaaacaata attgacgaat atagcgccac
    30601 gctgttcgca acctaaccaa acagtcactt tcgagcaatt ttccttgaaa aagaggttga
    30661 cgctgcaagg ctctatacgc ataatgcgcc ccgcaacgcc gataaggtat cgcgaaaaaa
    30721 agatggctac gtagctcagt tggttagagc acatcactca taatgatggg gtcacaggtt
    30781 cgaatcccgt cgtagccacc atcttttttg cgggagtggc gaaattggta gacgcaccag
    30841 atttaggttc tggcgccgca aggtgtgcga gttcaagtct cgcctcccgc accattcacc
    30901 agaaagcgtt gtacggatgg ggtatcgcca agcggtaagg caccggtttt tgataccggc
    30961 attccctggt tcgaatccag gtaccccagc catcttcttc gagtaagcgg ttcaccgccc
    31021 ggttattggg gtatcgccaa gcggtaaggc accggttttt gataccggca ttccctggtt
    31081 cgaatccagg taccccagcc atcgaagaaa taatctggct acgtagctca gttggttaga
    31141 gcacatcact cataatgatg gggtcacagg ttcgaatccc gtcgtagcca ccaaattctg
    31201 aatgtgtcga atacgttcgg caaattcaaa aaccaatttg ttggggtatc gccaagcggt
    31261 aaggcaccgg attctgattc cggcattccg aggttcgaat cctcgtaccc cagccaattt
    31321 attcaagacg cttacatcgt aagtgcaccc agttggggta tcgccaagcg gtaaggcacc
    31381 ggattctgat tccggcattc cgaggttcga atcctcgtac cccagccaca ttaaaaaagc
    31441 tcgcttcggc gagctttttg cttttctgcg ttcattcaat gtcgaatgcg atgttgacac
    31501 gtcttatcct tcaatgtcgg atgcgacgct gccgcgtctt atccgaccta cggctggcac
    31561 gcatccggca atgttgtaag gctacaaccc taacgcatat ttcagcgcct gacgtttcaa
    31621 cacgccagca cgctctgccg ccattaaccc gagattacgc acaaaacgca gcggcggcag
    31681 attattactg aatccggcat aaaacagatc cataccactt tgcataatga agttatccgc
    31741 catgcgccgc atctggtaac gcttgaggac aggataactg gcccacgctt caccgtagct
    31801 gcgggcattc accagaacat caatcagggc atcgacatca cgataaccaa gattcacccc
    31861 ctgccccgcc agcggatgga tggtatgcgc ggcatcgccc accagcgcaa gccccggctg
    31921 cacatactgc aacgcatgtc ggcgcgtcag cggaaacgca ccagcggcaa gcggtgtaac
    31981 gtaacccaga cgcgacggga aatgcttcgc gatttccgcc tggagttgcg ccatattcat
    32041 attctgcaac tggcgaatac gcgtcggaga gtcataccac accagcgatg cccagttatc
    32101 aaacaacggc agaaacgcac gcggtccgtc cggggtaaat tgctgccagg tgctgtcgcc
    32161 gggatcgttc tcacactgta cgctaatcaa catgcacgac tgcgcatact gccaggcatg
    32221 aacgccaatt cccgccatct gccgcacctg agaatttgcg ccgtcggcac caatcaccag
    32281 tttcgcgcgg atcgtttcac cgcccttcag ttccagctcc tgaagatcat tatctcgatg
    32341 taatgcaatc agcgaggttg gcacacgtaa cgttactttc ggatgcgctt ccagcgcctg
    32401 ccacaacgcc tgttgcagca ctgtgttttc caccatgtag ccaagcaacg gcaactttaa
    32461 ttcggctgcg tcaaatacca catgtgccgt ttcccactcc caggtttcca gtctgcggta
    32521 aggatggcaa cgcatagcct gtactgcatc ccagacccct aaccctttaa gcaatgatac
    32581 cgaagccgcg ctgatcgccg agatccgcac gtccggttgg ctgtcagcga caaacggtgc
    32641 aggttctgcg tgctcgatca ccgttaccga aaatccgtgc tgtgccagcc ccagcgccag
    32701 tgcgccgccg accattcctc cgccgacaat ggcaatttcc gttggttgat ttgtcatggt
    32761 caatcatcct gttagcaata tgctttaagt ttacaggatt tttaccccca agggctgata
    32821 acgctcatac tggtcacaac ggcagcaaag cattacacta tgcgccctgc attcctggct
    32881 actatttcgc aagagcaagt cgatgaccaa aaaactccat attaaaacct ggggctgtca
    32941 gatgaacgag tacgattcat cgaagatggc cgatctgctg gatgccaccc acggctatca
    33001 actgaccgac gtggcggaag aagcggatgt gctactgctg aacacctgct caatccgcga
    33061 gaaggctcag gaaaaagtct tccatcagtt gggtcgctgg aaactgttaa aagagaagaa
    33121 tccagacctg attatcggcg tcggtggctg cgtggcatcg caagaaggcg agcacattcg
    33181 ccagcgcgcc cactatgtcg atattatttt tgggccgcaa acgctgcacc gtctgccgga
    33241 gatgatcaac tccgtgcgcg gcgaccgcag cccggttgta gatatcagct tcccggaaat
    33301 cgagaagttt gaccgtctgc cggaaccgcg cgccgaaggg ccgaccgcgt ttgtctccat
    33361 catggaaggc tgcaataaat attgcaccta ctgcgtggtg ccttacaccc gtggtgaaga
    33421 ggtaagccgt ccgtctgacg atattctgtt tgagattgcc cagcttgcgg ctcagggcgt
    33481 gcgtgaagtc aacctgctcg gtcagaatgt gaatgcctgg cgtggtgaga actacgacgg
    33541 caccaccgga tcgtttgccg atctgttgcg tctggttgct gcgatcgacg ggatcgatcg
    33601 tattcgcttt accaccagcc atccgatcga attcaccgac gatatcatcg aagtgtaccg
    33661 cgacacgcct gagctggtga gcttcctgca tctgccggta cagagcggtt ccgatcgcat
    33721 tctgaacctg atgggacgta cccatacggc gctggagtac aaagcgatca tccgtaaact
    33781 gcgtgcggcg cgtccggata ttcagatcag ttctgacttc attgttggct tccctggcga
    33841 aaccaccgaa gacttcgaga aaacgatgaa gctgattgcc gacgtcaatt tcgacatgag
    33901 ctacagcttt atcttctctg cacgtccagg aacgcctgcc gccgatatgg ttgatgatgt
    33961 tccggaagaa gagaagaagc agcgtctgta tattctgcaa gaacgtatca atcagcaagc
    34021 gatggcatgg agccgtcgga tgctcggcac cacccagcgt attctggtag aagggacttc
    34081 gcgtaagagc atcatggagc tttccgggcg taccgaaaat aaccgcgtgg tcaacttcga
    34141 gggcacaccg gatatgatcg gtaaattcgt cgatgtagaa attaccgacg tctacccgaa
    34201 ctctctgcgc ggtaaagtgg tgcgaactga agatgaaatg ggtctgcgca tggcagaaac
    34261 accggagtcg gtgattgccc gcacccgcaa agaaaacgac cttggcgtgg gttattatca
    34321 gccgtaattc tcaggcctgc cgttccggtg ggccttgttt tttctctctt tatccccatc
    34381 tttatcgcaa tgcttgcgcc tttttcctgt ggcgtgaata atttccttat aggccggtga
    34441 atgttcagct tccggcggca tacagataag aggaacggtt tgaacataga cactcgcgaa
    34501 atcaccctgg agccagcaga caatgcgcgt ctgttgagcc tgtgcggccc gtttgatgac
    34561 aacatcaagc agctcgaacg ccgtctcggc atcgagatca atcgccgcga taaccacttt
    34621 aaactgaccg gccgtccgat ttgcgtcacc gctgcggcag acattctgcg tagcctgtat
    34681 gtcgatactg ccccgatgcg cggtcagatt caggatatcg aaccggaaca gatccacctt
    34741 gcgattaaag aagcgcgggt actggagcaa agcgcggaga gcgtgccgga gtacggcaaa
    34801 gcggtcaata tcaaaactaa acgcggcgta attaagccgc gcacgccaaa ccaggcgcag
    34861 tacatcgcca atattctcga ccatgacatt accttcggcg ttggcccggc gggtacgggt
    34921 aaaacctacc tggcagtggc tgcggcagtt gatgccctgg agcgtcagga aattcgccgt
    34981 attctgctga ctcgtccggc ggtcgaagcc ggtgagaaac tgggcttcct gcctggcgat
    35041 ttaagccaga aagtagaccc gtatctgcgc ccactgtacg acgcgctgtt tgaaatgctg
    35101 ggctttgaga aagtcgagaa actgattgag cgcaacgtta ttgaagtcgc gccgctggcc
    35161 tatatgcgtg gtcgtacgct gaacgacgcg tttatcattc tcgatgagag ccagaacact
    35221 accatcgaac agatgaagat gttcctgacc cgtatcggtt ttaactcaaa agcggttatc
    35281 accggcgacg tcacacagat cgacctgccg cgtaatacta aatcaggctt acgtcatgcc
    35341 attgaagtgc tggccgatgt cgaagagatc agctttaact tcttccacag cgaagacgtg
    35401 gttcgtcacc cggtggtggc gcgtatcgtt aacgcttatg aagcctggga agaagcagaa
    35461 caaaaacgta aagcggcgct ggcagcagaa cgcaagcgcg aagaacagga acaaaaatga
    35521 gtcaggtgat cctcgattta caactggcat gtgaagataa ttccgggtta ccggaagaga
    35581 gccagtttca aacatggctg aatgcggtga tcccgcagtt tcaggaagag tcggaagtga
    35641 ctattcgcgt ggtcgatacc gccgaaagcc acagtctgaa tctgacctat cgcggtaagg
    35701 ataagccgac caacgtgctc tccttcccgt ttgaagtgcc gcctggcatg gagatgtcgc
    35761 tactgggcga tctggttatc tgccgtcagg tggttgagaa ggaagctcag gagcaaggca
    35821 aaccactgga ggcgcactgg gcgcatatgg tggtgcacgg cagtctgcat ttgttaggtt
    35881 acgatcacat tgaagatgac gaagcagaag aaatggaagc cctcgaaaca gagattatgc
    35941 ttgctctggg ctatgaggat ccgtacattg ccgagaaaga ataatttgcc aggcatttga
    36001 ctggcagacg tccccgccgt tggtgattaa cgacggcgtt ttgattaact aacatgagaa
    36061 cccatacgac gccatgagcg acgacaattc acacagtagt gacacgataa gcaacaagaa
    36121 gggatttttc tccctgttac tcagccaact tttccacggt gaaccgaaaa accgtgacga
    36181 actgctggcg ctgatccgtg attccgggca gaacgacctt atcgacgaag atacgcgcga
    36241 tatgctcgaa ggggtgatgg acatcgcaga ccaacgcgtt cgcgacatca tgatcccccg
    36301 ctcccagatg attaccctga aacgcaacca gacgctggac gaatgccttg atgtcatcat
    36361 cgagtccgcc cactcacgtt tcccggtgat tagcgaagac aaagatcaca ttgaagggat
    36421 tctgatggcg aaagacttgc tgccgtttat gcgcagcgat gctgaagcct tcagcatgga
    36481 caaagtgtta cgtcaggcgg ttgtcgttcc tgaaagtaag cgcgtagacc ggatgctgaa
    36541 agagtttcgc tctcagcgtt accacatggc gatcgttatt gacgaattcg gtggggtttc
    36601 cggtctggtg accattgaag acatcctgga actgattgtt ggtgagattg aagacgagta
    36661 tgacgaagaa gatgatatcg acttccgtca gctgagtcgt catacctgga ccgtgcgcgc
    36721 actggcttcc attgaagact tcaacgaagc gttcggcacc cactttagcg atgaagaagt
    36781 cgacactatc ggtggtctgg tgatgcaggc gtttgggcat cttccggcgc gtggcgaaac
    36841 tatcgacatc gacggttacc agttcaaagt ggcgatggcc gatagtcggc gtattattca
    36901 ggttcatgtc aaaatcccgg atgactcacc ccagccaaag ctggatgaat aaaaccgaaa
    36961 ctggatagat aactacatgg attttgcctc attaattgaa cgccagcgca ttcgcctgct
    37021 gctggcgtta ttattcggtg cctgcggaac gctggccttc tctccttacg acgtctggcc
    37081 tgcggcgatt atttcgctga tggggcttca ggcgttgacc tttaaccgtc gcccactcca
    37141 gtctgccgct attggctttt gctggggatt tggcctcttt ggcagcggta ttaactgggt
    37201 ctatgtcagc atcgcgacct ttggcgggat gcctggcccg gttaacatct tcctggtggt
    37261 gctgctggcg gcgtatttgt cgctgtatac cggactgttt gctggcgtgc tgtcgcgtct
    37321 gtggccgaaa accacctggc tgcgcgtggc gattgccacc cctgctctct ggcaagtgac
    37381 cgagtttctg cgcggttggg tgctgactgg cttcccgtgg ttacagttcg gctatagcca
    37441 gattgatggc ccgttaaaag ggctggcacc gctaatgggc gtggaagcca ttaacttcct
    37501 gctgatgatg gttagtggcc tgctggcact ggcgttggtc aaacgcaact ggcgtccgct
    37561 ggtggtggcc gtcgtgctgt ttgccctacc cttcccgctg cgttacatcc aatggtttac
    37621 cccgcaaccg gagaaaacca ttcaggtttc gatggttcag ggcgatattc cgcaatcgct
    37681 gaaatgggac gaaggccagc ttcttaatac gctaaagatt tactacaacg caacggcacc
    37741 gctgatgggc aaatcatcgt tgattatctg gccggagtcg gcgataaccg atctggaaat
    37801 taatcagcaa ccgttcctca aagcactgga cggtgagttg cgtgataaag gtagctcgct
    37861 ggtaaccggg attgtcgacg cgcgtctcaa taagcagaac cgctacgata cctacaacac
    37921 catcatcacg ctgggtaaag gtgcaccgta cagctacgaa tcagccgatc gctataacaa
    37981 aaaccatctg gtgccgtttg gcgagtttgt cccgctggag tcgattctgc gtccgttagc
    38041 accgttcttt gatctgccga tgtcgtcgtt cagccgtggg ccatatatcc agccgccgct
    38101 gtcggcaaat ggtattgagc ttactgcggc tatttgctac gagattattc tcgccgagca
    38161 agtgcgcgat aacttccgcc cggataccga ctacctgctg actatctcca acgatgcgtg
    38221 gtttggtaaa tctattggtc catggcaaca cttccagatg gcgcgaatgc gtgcgctgga
    38281 gctggcgcgc ccactgttgc gcagcaccaa caacggcatt acggcggtga ttggcccgca
    38341 gggtgagatt caggcgatga tcccgcagtt cgcccgcgag gtgttaacca ctaacgtgac
    38401 gccgaccacc ggactcacac catacgcacg taccggcaac tggccgctgt gggtgctgac
    38461 ggcactgttt ggttttgctg ctgtgttgat gagtctgcgt cagcgacgta aataatccct
    38521 tcttatcgtg ccggaataca tcccatattt cggcacgctc accggatgcg gcatcagtgc
    38581 attatccgga taacaatatt ctcctatcca actttggcac atctattgct ttgttataca
    38641 aggcaaagct tgaaccagca tcagtacaac ccggttgcac cacagcaatc caccggtagc
    38701 accaaaagag tgcattttaa gaattttgct tcagaatggt gcggtgcacc tcgcaaaaac
    38761 aaacaataaa acatcaatat ctttacattt agccaaacta aatgttacct aacaggcaca
    38821 acactgcaca ataaagttgc agacgataac aacacaaaca ctcacaacgg gtatccatgc
    38881 gttcttaacg cagaagataa aggtgttgga tatgcaatta cgtaaacctg ccacagcaat
    38941 cctcgccctg gcgctttccg caggactggc acaggcagat gacgccgccc cggcagcagg
    39001 cagtacgctg gacaaaatcg ccaaaaacgg tgtgattgtc gtcggtcacc gtgaatcttc
    39061 agtacctttc tcttattacg acaatcagca aaaagtggtg ggttactcgc aggattactc
    39121 caacgccatt gttgaagcag tgaaaaagaa actcaacaaa ccggacttgc aggtaaaact
    39181 gattccgatt acctcacaaa atcgtattcc actgctgcaa aacggcactt tcgattttga
    39241 atgtggttct accaccaaca acgtcgaacg ccaaaaacag gcggctttct ctgacactat
    39301 tttcgtggtc ggtacgcgcc tgttgaccaa aaagggcggc gatatcaaag attttgccga
    39361 cctgaaaggc aaagccgtag tcgtcacttc cggcactacc tctgaagttt tgctcaacaa
    39421 actgaatgaa gagcaaaaaa tgaatatgcg catcatcagc gccaaagatc acggtgactc
    39481 tttccgcacc ctggaaagcg gtcgtgccgt tgcctttatg atggatgacg ctctgctggc
    39541 cggtgaacgt gcgaaagcga agaaaccaga caactgggat atcgtcggca agccgcagtc
    39601 tcaggaagcc tacggttgca tgttgcgtaa agacgatccg cagttcaaaa agctgatgga
    39661 tgacactatc gctcaggtgc agacctccgg tgaagcggaa aaatggtttg ataaatggtt
    39721 caaaaatcca attccgccga aaaacctgaa catgaatttc gaactgtcag acgaaatgaa
    39781 agcactgttc aaagaaccga atgacaaggc actgaactaa ttacaagaac caggggcgga
    39841 aattccagcc ctctcgattg ttacgtagca cggacagact atacgcctga tggtcgttcc
    39901 ccatcgggcc tgaaaaccgc aatacgctgg gtaacaatct tcgagggtag cagttaacgc
    39961 tgctaccctc ttttttctgg agtagattta tgtctataga ctggaactgg ggtatttttt
    40021 tacaacaagc cccgttcggc aataccacct atcttggttg ggtctggagt ggttttcagg
    40081 tgacgatcgc tttgtcgatc tgcgcctgga ttatcgcttt cctcgtcggt tcatttttcg
    40141 gcattttacg taccgttcca aaccgttttc tctccggtct gggtacgttg tatgtcgaac
    40201 tgttccgcaa cgtgccgttg attgtgcaat tctttacctg gtatctggtg atcccggagc
    40261 tgctgccgga gaaaatcggc atgtggttta aggccgagct ggatcccaat attcagtttt
    40321 tcctttcatc catgctctgc ctggggctgt ttaccgccgc ccgtgtttgc gaacaggtac
    40381 gcgccgctat tcagtcgcta ccgcgtgggc agaagaatgc cgcgctggcg atgggcctga
    40441 cgctaccgca gacttatcga tacgtactgc tgcctaatgc ttatcgcgtt atcgtcccgc
    40501 cgatgacttc cgagatgatg aacctggtga aaaactccgc catcgcctcg accatcggtc
    40561 tggtggatat ggcggcgcag gcgggtaaat tgctcgacta ctcggctcac gcctgggagt
    40621 cgtttaccgc catcacgctt gcgtatgtgt tgattaacgc ttttatcatg ctggtgatga
    40681 cgctggttga acgtaaagtt cgcctgcccg gcaacatggg gggcaaataa tgtacgagtt
    40741 tgactggagt tccattgttc cttccctgcc atatctgctc gacgggctgg tgatcaccct
    40801 gaaaatcacc gtcacggcag tcgtgattgg tattttgtgg ggtaccatgc tggcggtgat
    40861 gcgcttgtcc agcttcgcgc ctgtttcgtg gtttgccaaa gcctacgtta acgtattccg
    40921 ctcgattcct ttagtcatgg ttttgctgtg gttttacttg attgtgccgg gatttctgca
    40981 aaatgtgctg ggattatcgc caaaaaatga tattcgttta atttcggcga tggtggcgtt
    41041 ttcgatgttt gaagcggcct actattcaga gattatccgt gccggtattc aaagtatttc
    41101 ccgtggtcag tcgagcgccg cgctggcgtt ggggatgact cactggcagt cgatgaaact
    41161 gattattctg ccgcaggctt tccgcgcgat ggtgccgctg ctgctcactc agggcatcgt
    41221 actgttccag gatacctcgc tggtgtatgt gttatctctg gccgatttct tccgtaccgc
    41281 ctcaaccatt ggtgagcgtg atggtacgca ggtcgagatg atcctgtttg ccggatttgt
    41341 ttatttcgtt attagcctta gcgcgtcgtt gttggtcagc tacttgaaaa gaaggacagc
    41401 ataatgatta ccctgaaaaa tgtttcaaaa tggtatggtc actttcaggt gctgaccgac
    41461 tgctcaaccg aagtgaaaaa aggcgaagtg gtggtggttt gcggcccgtc tggttccggc
    41521 aaatcaacgc tgattaaaac cgtcaacggc ctcgaaccag tgcagcaagg tgaaatcacc
    41581 gtcgatggta tcgtggttaa cgacaagaaa accgatctgg caaagctgcg ctcccgcgtc
    41641 gggatggtat tccagcattt cgagctgttc cctcatctgt cgattatcga aaacctgacc
    41701 ctggcgcagg tgaaagtgct taaacgcgat aaagcgcccg ctcgtgaaaa agcattgaaa
    41761 ttgctggagc gcgtgggact ttccgcacat gccaataaat ttccggcgca gctttccggc
    41821 ggtcagcagc agcgcgtggc aatcgctcgc gcattgtgta tggctcctat tgcgatgctg
    41881 ttcgacgaac cgacatcggc gctggatccg gagatgatca acgaagtgct ggacgtgatg
    41941 gtggaactgg cgaacgaagg aatgaccatg atggtggtga cccacgaaat gggctttgcc
    42001 cgcaaagtgg cgaatcgggt gatctttatg gacgagggta aaattgtcga agactcgccg
    42061 aaagacgctt tcttcgatga tccgaaatcg gaccgcgcaa aagacttcct cgcgaaaatc
    42121 ctgcattaat cactctggcg cacataccca attgcgcgcc atctcacgct ttatcgtttc
    42181 gcccctcgta tctgttacat cctggcgtta cccttgtcgc aaagaagcac acaacaagga
    42241 gcaacaatgg cactgccaat tctgttagat tgcgacccag gtcatgatga cgctatcgca
    42301 atagttctcg cactcgcctc accagagctt gatgtcaaag caattacgtc ttccgccgga
    42361 aaccagacac cagaaaaaac cttacgcaat gttctgcgta tgctgacctt gcttaatcgc
    42421 accgatattc cggtggcagg cggcgcggta aaaccgttaa tgcgtgagtt gattatcgcg
    42481 gacaatgtgc acggcgaaag cggtctcgac ggcccggcat taccggaacc gacgttcgca
    42541 ccgcaaaact gtacggcggt ggagctgatg gcgaaaacgc tgcgcgaaag tgcggaacct
    42601 gtcacgattg tttctaccgg accgcaaact aacgttgcct tgctgctcaa tagccatccg
    42661 gaactgcata gcaaaattgc ccgtatcgtg attatgggcg gcgcaatggg gcttggtaac
    42721 tggacgcctg cggctgaatt taatatttac gttgacccgg aagcggcaga aattgtcttc
    42781 cagtcaggga tcccggtggt gatggccggt ctggatgtta ctcataaagc gcaaatccac
    42841 gttgaagaca ccgagcgttt ccgcgcgatt ggcaaccctg tttcaaccat tgttgccgaa
    42901 ctgctggatt tcttcctcga atatcataaa gacgaaaaat ggggctttgt cggcgcaccg
    42961 ctgcatgacc catgcaccat cgcctggctg ttgaaacccg agttgtttac cactgttgag
    43021 cgctgggttg gcgtggaaac acaggggaaa tatacccagg gtatgacggt tgttgattat
    43081 tattatctga ctggcaataa accgaatgcc actgtaattg tcgatgttga tcgtcagggc
    43141 tttgttgatt tactggctga tcgtctgaaa ttttacgctt aacactcgtc attgattaag
    43201 atcaccacgc gttacctggg tggtcttaat ctatttatct ctttttcttt caagatataa
    43261 atgcaatgga taatgcagaa ctcgccatag gtatcgatct cggcactacc aatagtttaa
    43321 ttgccgtctg gaaagacggt gccgcgcaat taattccaaa taagttcggt gaatatttaa
    43381 caccatccat aattagcatg gatgaaaata atcatatttt agtcggaaaa ccggctgtat
    43441 cacggcgtac ttcgcatccg gataaaacgg cagcgttatt taaacgtgca atgggcagta
    43501 ataccaactg gcggttaggc agcgacacat ttaacgcgcc agaactgtcc tctttggtat
    43561 tacgctcatt aaaagaagat gccgaagaat ttctgcaacg tccgattaaa gatgtggtga
    43621 tctccgttct ggcttatttc agcgatgaac aacgcaagca tacccgttta gcagcggact
    43681 tagccgggtt aaatgcggta cgcttaatta atgaacccac agcagctgcg atggcgtatg
    43741 gcctgcatac ccaacaaaat acccgttcgc tggtgtttga tctcggtggc ggcacgtttg
    43801 acgttacggt gcttgagtac gccacgccgg tgattgaagt tcacgcctcc gctggcgaca
    43861 acttcctcgg tggcgaagat tttacccata tgttggtcga tgacgtttta aagcgcgcgg
    43921 atgtcacaag gaccacgctt aacgagagtg aactggcagc cttgtacgcc tgtgtggaag
    43981 cggcaaaatg tagcaatcaa tcgccaatgc acattcgctg gtagtaccag gatgaaacgc
    44041 gggaatgcga attttacgag aacgaactgg aagatttgtg gttgccgctg ctcaatcgct
    44101 tgcgagtgcc gattgaacag gcgttgcgcg atgcgcgtct gaagccgagt caaatcgaca
    44161 gtctggtgct ggttggcggc gcgtcacaaa tgccgctggt gcagcgaatc gccgtgcgtc
    44221 tgtttggcaa attaccgtat caaagttacg atccgagcac cattgtcgcg ctgggcgcag
    44281 caatccaggc cgcctgccgc ttacgcagtg aagatattga agaagtgatc ctcactgata
    44341 tttgccctta ctcgttgggc gttgaggtta accgccaggg cgtttccggc attttctcgc
    44401 cgattattga acgaaacacc actgtgcccg tgtcgcgtgt agaaacttat tcaaccatgc
    44461 acccggaaca ggattcaatt acggttaacg tctatcaggg agaaaaccac aaagttaaaa
    44521 acaacattct ggtggaatcc ttcgatgtgc cgttgaagaa aaccggggct tatcagtcga
    44581 ttgatattcg ctttagttat gatatcaacg ggttgcttga agttgacgtg cttctggaag
    44641 acggcagcgt taagtccaga gtgattaacc acagcccggt aacattgagc gcgcagcaga
    44701 ttgaagagag tcggacgcgg ttatccgcat tgaaaattta tccgcgcgat atgctcatca
    44761 atcgcacctt taaagccaaa ctggaagagt tgtgggcgcg ggcgctgggt gacgagcgag
    44821 aagagattgg ccgggtgatc accgattttg atgcggcgct gcagtcaaac gatatggccc
    44881 gcgtcgatga ggtcaggcgg cgggcgagcg tttatttagc cattgagacc tcataatcac
    44941 ggctgccgga tgcggcgtaa atgctgcatc cggcatcggc gcaaaaaaca catcgtcaac
    45001 taaacaacat cgcgattttg ccgagagcat aacctataac cagcaccgtt aaactgattt
    45061 tgataatgct ggtaatgctc tgtggttttt caccctcatc atttttttct tcatggtttt
    45121 caggtgttgg ctcatcgaca tcttcatcct ccggctcgag ttttactttc gtcaacagca
    45181 tccgggtgat aatggcctct aatggatgag catctccgcc ttccagcctt gtattaagac
    45241 gggccagcat ctcttgtttc ggaaacgctt caagtactac ctgttccgct acccgataca
    45301 aatccagtaa cggtgaatct tcttctgtct cgctaccgac tctcgatttc caccacgccc
    45361 atgctttgcg tttgaagaat aacgactcaa taaacgattg acctaacggc gataattttc
    45421 cgctatgtaa aatttccgcc cataacagcc gcgtccgggc gctctcacca ggaacatatt
    45481 tcagcggttg accttgcgca tctaccatgc tccgcgcttc gataaccatc actaataacg
    45541 cgaaataatc cgggcaatga cgcttgctcc actgcaacaa caaattatca gcttgtgtag
    45601 atgggtaact ttcccagtac gcgccgagat cagcaagcag gctttccccc tcgccgcagt
    45661 acaaacgttg tgcacactgg taataccggg catcttcgct ttctggttct tccgcttgcc
    45721 actgtttggc tacaggaatg agttccacca tacctgtttg caccgagctg taccagcgca
    45781 acagtttgcg atgtaacttt gcatcatcag gaatgaacca cggtccttcc atcgccaaaa
    45841 acgcagtgac atagtcagga tggtaacggc agcaacgttc cagcgcgaag taatattcaa
    45901 cagtctggtt ctgtaacgcg acgggatatt ccagaaggct aaggaaatcg aaacaatcac
    45961 cagcctgaat ggcctcaaga aaagcctccc tttcttcctc atccattcct tcgctgtcat
    46021 taaacgaatg ccagttcaaa cgctctgcca acaaagaagc acaatttaat gacagatatc
    46081 gcgcttctat agcgattgca cactgcggcc agcgtaattg atcgacatcc tccatgttcc
    46141 aggtatttaa ttgctgaata aatttctgcc aggcggaggg ctgaaaacga tcactttctg
    46201 aatccagtaa tgtcctgaat gcacgtagta tttcatgttc agcggtggca tctttttctt
    46261 catcatctgc ttcctcgaca ggatttaccg ccagtcgcag cgcctcttca taggcctggc
    46321 gtaatgcttt aaacccctgc ggatcggttt cgggatgaca caacggtaag cgagcaagat
    46381 aagcctggcg gataatgtct atttgcgtcg tgctttcaat ttcgaggatt tgccaacatg
    46441 ttttcatagg tcgctcacat ccttaagcgt taccgggcat tcaggctgga cagttaatgg
    46501 ctgccaggga agcgtggcat agaaacggtc gcttcccgct gccagctctt cgaacatccg
    46561 actcccggcg ctatcgtatt tgtacagggt tagcgcctcg cgtagcgaag cgttgtcttc
    46621 gcactgagaa gattgccagt agagacgccc tagtgaatag gcagaaaaat agtgcatcca
    46681 gctgtgatag taatggtgag ctcgtgcata gacgcgcgat tgcagccata aactctcttc
    46741 ttccgtcagc cagttgttga gcacaccggc ccgtgacaga aatcccattc gaacataatc
    46801 ccaggctttg attccccctt ctccacagca catagcggtt tgggcaacga attgggtata
    46861 aatcaaccct ctctccgagc caccagcaat taatgctttc cactcttcag gggaagagcg
    46921 ttgccatgtc agatataagc cagctaaatc attggcatga ccatcatcag tcatctgata
    46981 aatagtctga attaaatcat cacgattatt tattccccag gatgatgaga gatcgaattc
    47041 ttttatcccg gtatacattt ttggcgagtc atgcgcgggt ttgcaactct gattaagtat
    47101 ttccatgggc gcagaaagcg caaaaagaag atattgttct tccttattca ttttttaata
    47161 atcccttaag tgcattaact gactactttt ttattattga tgagtataaa cttgacgctt
    47221 tcaaaataaa aagaaaatcg aagcattcac acatgaataa aaaattaatg tatatattcg
    47281 caatttttat agttgcagca attacctgta ttagccaacc caagaaaacg atgttgcgtg
    47341 ataaagccat ggtgaattat gcctttgatt atttaagatc accaggcagt ctaccgttaa
    47401 ccacggcagc cacggagctt tccgcgattc atggtcactc aacatcgcaa tatcgccttg
    47461 gcgaatttta tcttcatggt agcgacggta aaccactgga ttatacacag gcgagatact
    47521 ggtatgagca atcagcagaa caggaaaatc cgcgcgcaca aagcaaactg gggtggatct
    47581 acctgaaagg cctgggagtc aaacccgata cccgtaaagc aattctctgg tataaggaag
    47641 cagctgaaca agggtatgct catgctcaat atactttggg tttgatctac agaaatggca
    47701 ctggtattaa tgttaaccat tatgaatctc aaaaatggtt aaaactggcc gccaaacaac
    47761 attacaaaaa tgcggaaaga ttacttgccg gacttcccgc acattaacgt cgactgtcac
    47821 ccgaagagat ggagtatttt ccctatcaaa ccggcaaaga taaaaatgta gaaaataatt
    47881 ttgatgacgc ccagacagcc cagcccgcca tcatcaacgg gcattggctg cggttgcggc
    47941 tccggctcga cggtttcctg accggtaaaa ttcgccgcct gaattaataa tcgacgaata
    48001 attaatgctt ccagtggaca acgatcttct gcatcaaggc ggctcacaat cggcattaat
    48061 gattcttctg tcggttggct ggcctgcact aattgtttgg caactaaata taatggccat
    48121 aagggatgtt ctgtatcacg cgtgagtgag atccgcggct tacgtccttt gtgcaaaacc
    48181 atctcgacca gtgaacgccc cagcgcagag agtttttcat cgctgagtat ttcgtataaa
    48241 tgaaaacgcg tacgggcgct gtcgccgggg acataaagta gcggttttcc ctgatcgtta
    48301 accagatcac gcgcttcaat catcatcacc actaatgggt aataatagac ccgatgctga
    48361 cggcaccatt gcaacattaa agcatccgcc tgggtggagg gatactcgcg ccagtagtaa
    48421 caaagttctg ccagcaggct ttcgccttct ccacaataga cgcgttgtgc gtattcataa
    48481 taacgcgcat cttcattatc aggttcagct tcccgccacg accgggcgta atccaggagt
    48541 tcggggattc ccaggcggct tgcgctatac cagcggagta aatttctgtg cagttttgca
    48601 tcatcgggga taatcactgc gcgatgttgc gctaaataca atgtcagcca ttctggatgc
    48661 gatgaccaaa tccgagcgaa catttggtaa aaatcgatgg tgtcattttg cacggcaact
    48721 ggcagatgca gaatggtctg gaagttaaaa acattcccct tcgcaatggc atataaaaag
    48781 gattccagtt cttcttcgtc tatttccccg acgttttcct cctgcaacca ccgcaatctt
    48841 tctgctaata acaccacgca ctcgaaggat aaatgggcag tgttcatggc gattgtgcac
    48901 agcgaccagc gtaattcatc aatctcttcc atcgagcaat aatttaattg ctgaatgaat
    48961 cgctgccagg cggagggcag aaaacgttcg ctatcagagg caagtaacgc gcgaaaggcg
    49021 agcagaattt catgttctgc tacctcatat tcttctggtt gccaaacaga tttagcaggc
    49081 gactgcgcaa tccgtagcgc ctcctcatac gcctgacgaa gttgtttaaa accctgcgga
    49141 tcggtttctg gatgaaagga cggtaacagc gccagataag cgcggcggat aatatcgacg
    49201 tcagtcgttt cctctatatc gaggatcttc cagcaatttt tcataagtcg ctcatatcct
    49261 taagcgtttc cgggcatgtg gggtagtcag ccagtggttg ccaggacaag gtagggtaat
    49321 acgcatcatc actggcaaat aattgataaa acatatcatt gccggaatcg tcgtattctt
    49381 tacgcgctaa gaattcgcgt aataaaggca gatgatcatc ttcgggagac tgccaatatt
    49441 gccgaccaaa ggtataaccg gcaaagtatt gccgccagtt actataataa cgtagagccc
    49501 gtagatgaat acgcgactgg atccatagac tttcttcttc actcaaccag ttattaagta
    49561 cgcccattcg acttaaaaaa cccatccgga cataatccca tgctttaatt cctccctcac
    49621 cacagcattc ggctgtgctg gcgacaaatt gcgcataagc ctgaccttgc tcatttaatt
    49681 cagcaagata gtcacgccat tcacatggag aataacgaaa ccagcggtga taaaaccctg
    49741 ccaaccgagc ggcatgaccg ttctcgatca ttcggtgaat tatgcgcagc aggtcatcac
    49801 ggtcatcaat tccccatgag gaagaaaggt caaaaaagca tgtacctaaa tacattttgg
    49861 gggatgaatg cgaaggtaaa cagcattcat tgcgtatttc catcggagtg gaaagtgcaa
    49921 acaatatttt ttgcgacccc atgtccatat aatttatcct taaacattca tttaatgaat
    49981 gtaaactaca tttcgaggct cagttattta tacttttctg acgcaacata atacgcataa
    50041 aataaggctt acgcaaaaat ggaacacata aaaatataat atattttatc tacaaccata
    50101 aggaaatgat aatgattttt acgtcaagtt gctgcgataa tttatcaata gatgagatta
    50161 tcgaacgtgc tgaaaaaggc gattgcgagg ctcaatatat tgttgggttt tattataatc
    50221 gcgatagcgc aattgactct ccggacgacg aaaaggcctt ttactggctg aagctggccg
    50281 ctgagcaagg tcattgtgaa gcacagtatt ccttagggca aaagtatacc gaggataaaa
    50341 gccgtcataa agataatgag caagccatct tctggctgaa aaaagctgcc ctacaaggcc
    50401 atactttcgc ttccaacgcc cttggctgga tactggatcg tggagaagac cccaactata
    50461 aagaagcggt tacctggtat cagatagccg cggagagcgg aatgtcttat gcgcaaaata
    50521 atcttggatg gatgtacaga aatggcaacg gagtcgcaca agactatacg ctggcatttt
    50581 tctggtacaa acaagctgca ttacaaggcc atagttacgc acaagacaat ctggccgatc
    50641 tttataaaga cggagaaggc gttgctcaaa acaagacact cgccgcattc tggtatttga
    50701 aaagcgcaca gcagggtaat cggcacgccc agtttcaaat tgcatgggat tataacgctg
    50761 gcgaaggggt ggaccaggac tataagcaag cgatgtactg gtatctgaag gctgccgctc
    50821 aggaaagcgt cggcgcttac gtcaacatcg gttatatgta taaacacgga caaggcgttg
    50881 agaaagatta tcaggctgcc tttgaatggt ttacgaaagc cgctgaatgc aatgacgcca
    50941 ctgcctggta taacctggcc attatgtatc actacggaga aggaagacct gtcgatctcc
    51001 gacaggctct cgacctgtat cgtaaagttc agtcatccgg aaccagagat gtcagtcagg
    51061 aaattcgtga gactgaagct ttactgtaga aataaaaaca agggccgggg aatttcattc
    51121 tgcccatatt ctctttatta ctgtgcgaaa tcagactatt acggctcaaa cgggcgtctc
    51181 tggaactggt cataaccaca tttcgggcat agcgtcagca cttccggtgt gtagatcggg
    51241 agatggaagt gacatttctc gcagaccaga tttcccagcc cgaccacttc tccgctgtga
    51301 taaaccccat gatgattgag gtcctggaaa atttcgcgcc attcaagctg cgttttatcg
    51361 gtgatgtctg ccagctcctg ccacaagctt tctttaatca cccgcataaa gacgctgtca
    51421 gattcttctt tcaggctctc ttcatagctc atggcgaact cttccaggtc acgtctgaca
    51481 gctcgcgtca gctcatcgat ctcggttcgc gttaactccc ctgtttttat tacgcgctcg
    51541 cgcgcctgtt ccaccagtgc gtcgatatca cgttcgccat tgcgcaggcg ttcgttcagt
    51601 gacgcgacca gttcacggta atattgagca accttgttca tcgtttcatc tcccgggtcg
    51661 ttaactgact ataataatag acgttctttg ttccttgctt tgctaatacg cccacaaact
    51721 ctgtaaatta atgtacgaat aatccgggca tcatttcaac gtcgagtgcg cgaaaggctg
    51781 ttttgacgcg gacgtttggg ctatgctatg cggatctgaa aaaccacctc aacgctacat
    51841 ttgtagccgt attgaaaaca ggaccactgg ctgccatgca agagcaatac cgcccggaag
    51901 agatagaatc caaagtacag cttcattggg atgagaagcg cacatttgaa gtaaccgaag
    51961 acgagagcaa agagaagtat tactgcctgt ctatgcttcc ctatccttct ggtcgactac
    52021 acatgggcca cgtacgtaac tacaccatcg gtgacgtgat cgcccgttac cagcgcatgc
    52081 tgggcaaaaa cgttctgcag ccgatcggct gggacgcgtt tggtctgcct gcggaaggcg
    52141 cggcggtgaa aaacaacacc gctccggcac cgtggacgta cgacaacatc gcgtatatga
    52201 aaaaccagct caaaatgctg ggctttggtt atgactggag ccgcgagctg gcaacctgta
    52261 cgccggaata ctaccgttgg gaacagaaat tcttcaccga gctgtataaa aaaggcctgg
    52321 tatataaaaa gacttctgcg gtcaactggt gcccgaacga ccagaccgta ctggcgaacg
    52381 aacaagttat cgacggctgc tgctggcgct gcgataccaa agttgaacgt aaagagatcc
    52441 cgcagtggtt tatcaaaatc actgcttacg ctgacgagct gctcaacgat ctggataaac
    52501 tggatcactg gccagacacc gttaaaacca tgcagcgtaa ctggatcggt cgttccgaag
    52561 gcgtggagat caccttcaac gttaacgact atgacaacac gctgaccgtt tacactaccc
    52621 gcccggacac ctttatgggt tgtacctacc tggcggtagc tgcgggtcat ccgctggcgc
    52681 agaaagcggc ggaaaataat cctgaactgg cggcctttat tgacgaatgc cgtaacacca
    52741 aagttgccga agctgaaatg gcgacgatgg agaaaaaagg cgtcgatact ggctttaaag
    52801 ctgttcaccc attaacgggc gaagaaattc ccgtttgggc agcaaacttc gtattgatgg
    52861 agtacggcac gggcgcagtt atggcggttc cgggtcacga ccagcgcgac tacgagtttg
    52921 cctctaaata cggcctgaac atcaaaccgg ttatcctggc ggctgacggc tctgagccag
    52981 atctttctca gcaagccctg actgaaaaag gcgtgctgtt caactctggc gagttcaacg
    53041 gtcttgacca tgaagcggcc ttcaacgcca tcgccgataa actgactgcg atgggcgttg
    53101 gcgagcgtaa agtgaactac cgcctgcgcg actggggtgt ttctcgccag cgttactggg
    53161 gcgcgccgat tccgatggtg acgctggaag acggtaccgt aatgccaacc ccggacgacc
    53221 agctgccggt gatcctgccg gaagatgtgg tgatggacgg cattaccagc ccgattaaag
    53281 cagatccgga gtgggcaaaa actaccgtta acggtatgcc agcgctgcgt gaaaccgaca
    53341 ctttcgacac ctttatggag tcctcctggt actatgcgcg ctacacttgc ccagagtaca
    53401 aagaaggtat gctggattcc gaagcggcta actactggct gccggtggat atctacattg
    53461 gtggtatcga acacgccatt atgcacctgc tctacttccg cttcttccac aaactgatgc
    53521 gtgatgcagg catggtgaac tctgacgaac cagcgaaaca gttgctgtgt cagggtatgg
    53581 tgctggcaga tgccttctac tatgttggcg aaaacggcga acgtaactgg gtttccccgg
    53641 ttgatgctat cgttgaacgt gacgagaaag gccgtatcgt gaaagcgaaa gatgcggcag
    53701 gccatgaact ggtttatacc ggcatgagca aaatgtccaa gtcgaagaac aacggtatcg
    53761 acccgcaggt gatggttgaa cgttacggcg cggacaccgt tcgtctgttt atgatgtttg
    53821 cttctccggc tgatatgact ctcgaatggc aggaatccgg tgtggaaggg gctaaccgct
    53881 tcctgaaacg tgtctggaaa ctggtgtacg agcacacagc aaaaggtgat gttgcggcac
    53941 tgaacgttga tgcgctgact gaagatcaga aagcgctgcg tcgcgatgtg cataaaacta
    54001 tcgctaaagt gaccgatgat atcggccgtc gtcagacctt caacaccgca attgcggcga
    54061 ttatggagct gatgaacaaa ctggcgaaag caccgaccga tggcgagcag gatcgcgctc
    54121 tgatgcagga agcactgcag gccgttgtcc gtatgctcaa cccgttcacc ccgcacatct
    54181 gcttcacgct gtggcaggaa ctcaaaggcg aaggcgatat cgacaacgcg ccgtggccgg
    54241 ttgctgacga aaaagcgatg gtggaagact ccacgctggt cgtggtgcag gttaacggta
    54301 aagtccgtgc caaaatcacc gtcccggtgg acgcaacgga agaacaggtt cgcgaacgtg
    54361 ctggccagga acatctggta gcaaaatatc ttgatggcgt tactgtacgt aaagtgattt
    54421 acgtaccagg taaactcctc aatctggtcg ttggctaagc gcgggaggaa gcgtgcgata
    54481 tctggcaaca ctgttgttat ctctggcggt gttaatcacc gccgggtgtg gctggcatct
    54541 gcgtgacacc acgcaggttc cttccactat gaaggtcatg atcctggact caggcgatcc
    54601 gaacgggcca ttaagccgtg cggtgcgtaa ccagttacgt ctgaatggtg tcgagttgct
    54661 tgataaagaa accacgcgta aggacgttcc atccctgcgt ttgggtaaag tgagcatcgc
    54721 gaaagatacc gcatcggtat tccgtaacgg tcaaacagca gagtatcaga tgatcatgac
    54781 ggtgaatgcg accgtgttga tccccggccg tgatatctac ccgattagcg ccaaagtctt
    54841 ccgttcattc ttcgataacc cgcaaatggc gttagcgaaa gataacgaac aagacatgat
    54901 cgtaaaagag atgtacgacc gtgctgccga acagctgatt cgtaagctgc caagcatccg
    54961 tgctgcggat attcgttccg acgaagaaca gacgtcgacc acaacggata ctccggcaac
    55021 gcctgcacgc gtctccacca tgctgggtaa ctgatgattc ggttgtaccc ggaacaactc
    55081 cgcgcgcagc tcaatgaagg gctgcgcgcg gcgtatcttt tacttggtaa cgatcctctg
    55141 ttattgcagg aaagccagga cgctgttcgc caggtagctg cggcacaagg attcgaagaa
    55201 caccacactt tttccattga tcccaacact gactggaatg cgatcttttc gttatgccag
    55261 gctatgagtc tgtttgccag tcgacaaacg ctattgctgt tgttaccaga aaacggaccg
    55321 aatgcggcga tcaatgaaca acttctcaca ctcaccggac ttctgcatga cgacctgcta
    55381 ttgatcgtcc gcggtaataa attaagcaaa gcgcaagaaa atgccgcctg gtttactgct
    55441 cttgcgaatc gcagcgtgca ggtgacctgt cagacaccgg agcaggctca gcttccccgc
    55501 tgggttgctg tgcgcgcaaa acagctcaac ttagaactgg atgacgcggc gaatcaggtg
    55561 ctctgctact gttatgaagg taacctgctg gcgctggctc aggcactgga gcgtttatcg
    55621 ctgctctggc cagacggcaa actgacatta ccgcgcgttg aacaggcggt gaatgatgcc
    55681 gcgcatttca ccccttttca ttgggttgat gctttgttga tgggaaaaag taagcgcgcg
    55741 ttgcatattc ttcagcaact gcgtctggaa ggcagcgagc cggttatttt gttgcgcaca
    55801 ttacaacgtg aactgttgtt actggttaac ctgaaacgcc agtctgccca tacgccactg
    55861 cgtgcgttgt ttgataagca tcgggtatgg cagaaccgcc ggggcatgat gggcgaggcg
    55921 ttaaatcgct taagtcagcc gcagttacgt caggccgtgc aactcctgac acgaacggaa
    55981 ctcaccctca aacaagatta cggtcagtca gtgtgggcag agctggaagg gttatctctt
    56041 ctgttgtgcc ataaacccct ggcggacgta tttatcgacg gttgatatga aatctttaca
    56101 ggctctgttt ggcggcacct ttgatccggt gcactatggt catctaaaac ccgtggaaac
    56161 gctggcgaat ttgattggtc tgacgcgggt cacaatcatc cctaataatg ttcctccgca
    56221 tcgtccccag ccggaagcga acagcgtgca gcgtaaacac atgcttgaac tggcgattgc
    56281 cgacaagcca ttatttactc ttgatgaacg cgagctaaag cgcaatgccc cctcttacac
    56341 tgcgcaaaca ctgaaagagt ggcggcagga acaaggaccg gacgtgccgc tggcgtttat
    56401 tattggtcag gattcattgc tgacctttcc gacctggtac gaatacgaaa cgatactcga
    56461 caatgcacat ttgatcgtct gtcggcgtcc aggttaccca cttgaaatgg cgcaaccgca
    56521 ataccagcaa tggctggaag atcatttgac acataacccg gaagatcttc accttcagcc
    56581 tgccggtaaa atttatctgg ctgaaacgcc gtggtttaac atctcggcga ccatcatccg
    56641 cgaacgtttg caaaacggtg aatcgtgtga ggatttattg ccggaaccgg tattgactta
    56701 cattaaccaa caaggcttgt atcgctgata cctgtcgtta cggagggcat gatgcgactg
    56761 tggttaattc gtcatggcga aacacaagcg aatgtcgatg gtctgtacag cggtcatgcg
    56821 cccacacccc tgaccgcacg cggtattgag caagcgcaaa atctgcatac actgctagat
    56881 gatgtttcct ttgacttggt tttatgcagt gaactggaac gggcacagca taccgcgcga
    56941 ctggttctca gtgaccgcca gctccccgtt catatcatcc ctgaactcaa cgaaatgttt
    57001 tttggcgact gggagatgcg acatcatcgc gacctcatgc aagaagatgc cgaaaactat
    57061 agcgcgtggt gcaatgactg gcagcatgct atccccacga acggtgaagg atttcaggca
    57121 ttttcgcaac gtgtggaacg ctttatcgca aggcttagtg aatatcagca ctatcagaat
    57181 attttaatcg tcagccatca gggcgtactg agtctgttaa ttgctcgttt aattggcatg
    57241 cctgccgagt ctatgtggca ttttcgcgtt gaccaggggt gttggagcgc cattgatatc
    57301 aaccaaaaat tcgcgacgct acgcgtcctc aatagccgtg ccatcggggt cgagaacgca
    57361 tgacttttct gtttttttac gggtaagccg caacgaccat tgacagcccc gggcaggctg
    57421 atattctccg cagccagact ttttccgcca gacacgactt tgtagaaatt gttttacaaa
    57481 aatggcgatg caatctgcgg cgcggggtgg gatgatagcc cactttcgaa agccgattcg
    57541 gcgacaattg tcccgaaatc gcctctggtt caggtatact gacagaccat ttttatctat
    57601 ttgattcacc cagggggaaa acttgcaggg taaagcactc caggattttg ttatcgacaa
    57661 aattgatgac ctcaaaggtc aggacatcat cgccttagac gttcaaggca aatccagcat
    57721 caccgactgc atgatcatct gtacgggtac gtccagccgt catgttatgt ccattgctga
    57781 ccacgttgtg caggagtctc gcgcagcggg cctgttaccg ctcggcgtag aaggtgaaaa
    57841 cagcgccgac tggattgtcg tggatttggg cgatgtgatt gtccatgtca tgcaggaaga
    57901 gagccgtcgc ctgtatgaac tggaaaaact ctggagttaa tgcgtgaagc tgcaacttgt
    57961 cgccgtggga acgaaaatgc cggactgggt acaaaccggt tttaccgagt acctgcgtcg
    58021 ttttccgaaa gatatgccct tcgagctgat tgaaattccg gccggcaaac gcggcaagaa
    58081 tgcggacatc aagcgcatac tcgacaaaga gggtgagcag atgttggcgg ccgcaggcaa
    58141 aaaccgcatt gtcaccctcg atattccagg caagccctgg gatacgccgc agttagccgc
    58201 tgagctggaa cgctggaagc tggatggtcg cgacgtaagt ctactgattg gcgggcctga
    58261 agggttgtcg cctgcctgta aagcggcggc tgagcagagc tggtcgctgt cggcgcttac
    58321 cctcccccat ccgctggttc gcgtgctggt cgcagagagt ctgtaccggg cgtggagcat
    58381 caccaccaac catccttatc accgtgagtg ataagggagc tttgagtaga aaacgcagcg
    58441 gatgaaacta cagaactctt ttcgcgacta tacggcagag tccgcgctgt ttgtgcgccg
    58501 ggcgctggtc gcctttttgg ggattttgct gctgaccggc gtgcttatcg ccaacctgta
    58561 taatctgcaa attgttcgct ttaccgacta ccagacccgc tctaatgaaa accgcattaa
    58621 gctggtgcct atcgcgccca gccgcggcat tatctacgac cgtaacggta tccctctggc
    58681 cctcaaccgc actatctacc agatagaaat gatgccggag aaagtcgata acgtgcagca
    58741 aacgctggac gctttgcgca gcgtggtaga tctgaccgat gacgatattg ctgcattccg
    58801 aaaagagcgc gcacgttcac accgtttcac ctctattccg gtgaaaacta acctaaccga
    58861 agtacaagta gctcgctttg ccgtcaatca gtaccgtttt ccgggtgtcg aagttaaagg
    58921 ctataaacgt cgttactatc cttacggttc ggcgttgacc cacgtcatcg gctatgtgtc
    58981 gaaaatcaac gataaagacg tcgaacgcct gaataatgac ggcaaactgg ccaactatgc
    59041 ggcaacgcat gatattggta agctgggcat tgagcgttac tatgaagacg tgctgcacgg
    59101 tcagaccggt tatgaagagg ttgaagttaa caaccgtggg cgtgttatcc gccagttaaa
    59161 agaagtacca ccgcaagccg gacacgatat ttacctgacg ctggatctca aactccagca
    59221 atatattgaa acgctgctgg cgggtagccg cgcagctgtg gttgttaccg aaccgcgtac
    59281 aggtggggtg ctggcgctgg tttccacgcc tagttatgac ccaaacctgt ttgttgacgg
    59341 tatctccagc aaagattatt ccgccttgct gaacgacccg aatacaccgc tggtgaaccg
    59401 cgccacacag ggggtttatc ctcccgcgtc tacagttaaa ccctatgtgg cggtttcggc
    59461 attaagcgcc ggggtgatca cgcgcaatac gacgctgttt gacccaggct ggtggcaact
    59521 accagggtcg gaaaaacgtt atcgtgactg gaaaaaatgg ggccacgggc gtctgaatgt
    59581 cacaagatcg ctggaagaat ctgcggatac cttcttctat caggtggcct acgatatggg
    59641 gatcgatcgc ctctccgaat ggatgggtaa attcggttat ggtcattaca ccggtatcga
    59701 cctggcggaa gaacgttccg gcaacatgcc tacccgcgaa tggaaacaga aacgctttaa
    59761 aaaaccgtgg tatcagggtg acaccattcc ggttggtatc ggccagggtt actggacagc
    59821 gaccccaatc cagatgagta aggcactgat gatcctgatt aatgacggta tcgtgaaggt
    59881 tccgcatttg ctgatgagca ccgccgaaga cggcaaacag gtgccatggg tacaaccgca
    59941 tgaaccgccc gtcggcgata ttcattccgg ttactgggag ctggcgaaag acggtatgta
    60001 cggtgttgct aaccgcccta atggtacggc gaataaatac tttgccagcg caccgtacaa
    60061 aattgcggcg aaatctggta ccgctcaggt cttcggtctg aaagcgaacg aaacctataa
    60121 tgcgcacaaa attgccgagc gtttacgtga ccacaaactg atgaccgcct ttgcgccata
    60181 caacaatccg caagtggctg tcgccatgat tctggagaac ggcggcgcgg gtccggcggt
    60241 tggtacgctg atgcgccaga tcctcgacca cattatgctg ggtgataaca acaccgatct
    60301 gcctgcggaa aatccagcgg ttgccgcagc ggaggaccat taatcatgac ggataatccg
    60361 aataaaaaaa cattctggga taaagtccat ctcgatccca caatgctgct gattttactg
    60421 gcattgctgg tttacagcgc cctggttatc tggagcgcca gcggtcagga tattggcatg
    60481 atggagcgta aaatcggcca aatcgcgatg ggtctggtca tcatggtggt gatggcgcaa
    60541 attcctccac gcgtttatga aggctgggcc ccctatctct atatcatctg tattattttg
    60601 ctggtggcgg tagatgcttt cggagccatc tctaaaggcg ctcaacgctg gctggacctc
    60661 ggtattgttc gttttcagcc gtcggaaatt gccaaaatag ccgtaccact gatggttgcg
    60721 cgctttatca accgcgacgt ttgcccgcca tcgttgaaga atactggcat cgcgctggtg
    60781 ctgatattta tgcccacgct gctggtggct gcacagcctg acctgggaac atcaatcctc
    60841 gttgcgcttt ccggtctgtt tgtactgttc ctctctggcc ttagctggcg tctgattggc
    60901 gtcgcagtag tgctggtagc ggcgttcatt ccgattctgt ggttcttcct gatgcatgat
    60961 taccagcgcc agcgcgtaat gatgctcctg gacccggaat cagacccact cggcgcgggc
    61021 tatcacatta ttcagtctaa aattgctatt ggctccggcg gattacgcgg caaaggctgg
    61081 ctgcacggca ctcagtcaca gcttgaattt ctccccgaac gccatactga ctttatcttc
    61141 gcggtactgg cggaagagct gggattagtg ggcattctga ttctgctcgc tctctacatt
    61201 ctgctgatca tgcgcgggct gtggatagcc gccagagcgc aaaccacctt tggtcgcgtc
    61261 atggctggtg gcttaatgct gatattattc gtttatgtct tcgtaaatat tggtatggta
    61321 agcggtattc tgccggttgt aggggttccg ctcccactgg tcagttatgg aggatcggcg
    61381 ctaattgtgc tgatggctgg gttcgggatt gtaatgtcaa tccacaccca caggaaaatg
    61441 ttgtcgaaaa gcgtgtaaga ggtgcgcaat gcgtaagcag tggctcggga tctgcatcgc
    61501 ggcaggaatg ctcgcggcat gtacaagcga tgatggtcag caacagacgg taagtgtacc
    61561 gcagcctgcg gtatgtaacg gccccatagt tgaaattagc ggggcggacc cgcgtttcga
    61621 accactgaac gcgacggcaa atcaggatta ccagcgcgac ggtaaaagct acaaaatcgt
    61681 gcaggatccg tctcgattta gccaggcggg actggcggca atctatgatg ccgaaccagg
    61741 cagtaacctg acggcctctg gcgaagcttt cgatccgaca aagctgacgg cggcccatcc
    61801 aacgcttccg atccccagct acgccagaat cactaacctg gctaacgggc gaatgatcgt
    61861 ggtgcgcatt aatgatcgcg gcccttacgg caacgaccgc gttatttcgc tttctcgcgc
    61921 ggcagctgac cgtcttaaca cgtcaaacaa caccaaagtt cgtatcgatc cgattattgt
    61981 cgcccaggat ggttcgcttt ctggtcctgg tatggcttgt accacagtcg ccaaacagac
    62041 ttacgccctg cctgcacctc ccgatttaag cggtggcgcg ggaacaagtt cagtgtctgg
    62101 cccgcagggt gacattcttc cggtcagtaa ttcgacgcta aaaagcgaag atccgaccgg
    62161 cgcgccggta accagtagcg gtttcctcgg cgctccaacg accttagcgc ctggtgtact
    62221 ggaaggcagc gaaccgacgc ctgcgccaca gcccgttgtt acagcttcgt cgacaacgcc
    62281 tgcaacctcg cctgcaatgg tgacaccgca agccgcctcg caaagcgcca gcggcaactt
    62341 tatggtgcaa gtcggggccg taagcgatca ggctcgtgcg caacagtacc aacagcaact
    62401 gggacagaag ttcggcgtcc ccggtcgcgt aactcaaaat ggcgcggtct ggcggatcca
    62461 gcttggccca ttcgccagca aagccgaagc cagtaccttg cagcaacgtt tgcaaaccga
    62521 agcccaatta cagtcattta ttactaccgc gcagtagcgt aaagcaggca tctgatgtgt
    62581 caatttctgt aaatgacgtt aacaaagtca cgcggaaagt cagatgcctg ccggtagtgc
    62641 atttgctata gtagggcact tttttaattc catcacggat gtcgtagttc agaccatgaa
    62701 taccattttt tccgctcgta tcatgaagcg cctggcgctc accacggctc tttgcacagc
    62761 ctttatctct gctgcacatg ccgatgacct gaatatcaaa actatgatcc cgggtgtacc
    62821 gcagatcgat gcggagtcct acatcctgat tgactataac tccggcaaag tgctcgccga
    62881 acagaacgca gatgtccgcc gcgatcctgc cagcctgacc aaaatgatga ccagttacgt
    62941 tatcggccag gcaatgaaag ccggtaaatt taaagaaact gatttagtca ctatcggcaa
    63001 cgacgcatgg gccaccggta acccggtgtt taaaggttct tcgctgatgt tcctcaaacc
    63061 gggcatgcag gttccggttt ctcagctgat ccgcggtatt aacctgcaat cgggtaacga
    63121 tgcttgtgtc gccatggccg attttgccgc tggtagccag gacgcttttg ttggcttgat
    63181 gaacagctac gttaacgcac tgggcctgaa aaatacccac ttccagacgg tacatggtct
    63241 ggatgctgat ggtcagtaca gctccgcgcg agatatggcg ctgatcggcc aggcattgat
    63301 ccgtgacgta ccgaatgaat actcgatcta taaagaaaaa gaatttacgt ttaacggtat
    63361 tcgccagctg aaccgtaacg gcctgttatg ggataacagc ctgaatgtcg acggcatcaa
    63421 aaccggacac actgacaaag caggttacaa tcttgttgct tctgcgactg aaggccagat
    63481 gcgcttgatt tctgcggtga tgggtggacg tacttttaaa ggccgtgaag ccgaaagtaa
    63541 aaaactgcta acctggggct tccgtttctt cgaaaccgtt aacccactga aagtaggtaa
    63601 agagttcgcc tctgaaccgg tttggtttag tgattctgat cgcgcttcgt taggggttga
    63661 taaagacgtg tacctgacca ttccgcgtgg tcgcatgaaa gatctgaaag ccagctatgt
    63721 gctgaacagc agtgaattgc atgcgccgct gcaaaagaat caggtcgtcg gaactatcaa
    63781 cttccagctt gatggcaaaa cgatcgagca acgcccgctg gttgtgttgc aagaaatccc
    63841 ggaaggtaac ttcttcggca aaatcattga ttacattaaa ttaatgttcc atcactggtt
    63901 tggttaaaaa ttgaacactt gaaagtgtaa tttccgtccc catatactaa gcatcagtaa
    63961 aaaaactccc gccttctggc gggagttgct atttaattac gttacgccgg agctgacatg
    64021 aaaaccaaac ttaacgaact gcttgaattc cctactcctt ttacttacaa agttatgggg
    64081 caggcgttac ctgagctggt tgatcaggtg gttgaagtgg tacagcgcca tgcgccaggt
    64141 gactacaccc caacggtaaa accaagcagc aaaggcaact accactcggt atctatcact
    64201 atcaacgcca ctcatatcga gcaggttgaa acactgtatg aagaactggg caaaatcgat
    64261 attgtccgca tggttctgta actcgcttct ccgttacccg gctcctggtc gggtaactcc
    64321 cctctcctcg ctgtgatata cttttccccc acttttactc attctccacg gagatgccgt
    64381 tttgtatcag gataaaattc ttgtccgcca gctcggtctt cagccttacg agccaatctc
    64441 ccgggctatg catgaattca ccgatacccg cgataatagt acccttgatg aaatctggct
    64501 ggtcgagcac tatccggtat tcacccaagg tcaggcagga aaagcggagc acattttaat
    64561 gccgggtgat attccggtga tccagagcga tcgcggtggg caggtgactt atcacgggcc
    64621 gggacaacag gtgatgtatg tgttgcttaa cctgaaacgc cgtaaactcg gtgtgcgtga
    64681 actggtgacc ttgcttgagc aaacagtggt gaataccctg gctgaactgg gtatagaagc
    64741 gcatcctcgg gctgacgcgc ctggtgtcta tgtcggggaa aagaaaattt gctcactggg
    64801 tttacgaatt cgacgcggtt gttcattcca cggtctggca ttaaacgtca atatggatct
    64861 ttcaccattt ttacgtatta atccttgtgg gtatgccgga atggaaatgg ctaaaatatc
    64921 acaatggaaa cccgaagcga cgactaataa tattgctcca cgtttactgg aaaatatttt
    64981 agcgctacta aacaatccgg acttcgaata tattaccgct taattccata catcaatggc
    65041 ccaatttaca ctgggtcatt actcataata cctgctaatg ctttacatct ccttcgcgtt
    65101 tgtatatttt ctttatcact tgcagaaatg cggtgccacg ccttgctccg taactcaccc
    65161 cattgattaa cctattgaaa tagttgatta tggtgagcaa aagcgttccg ttccaattag
    65221 acataagatt attgtaattt tttaaatatt atttttccat gaataaaatt ggagtaagtg
    65281 cgtggatagt aataatcaaa ttgaaccctg tttaagcaga aagtcgtcag aaggtaaacc
    65341 acaaatattt acaaccttac gaaatattga tcttaacctt ctgactattt ttgaagctgt
    65401 atatgtacat aaagggatcg ttaatgcagc gaaagtgctt aatctgaccc cctcggcaat
    65461 cagtcagtct attcagaaac tgcgcgttat attccctgac ccattgttta ttcgcaaagg
    65521 ccagggtgtc actcctaccg catttgcgat gcatctacat gagtatatca gtcagggcct
    65581 tgagtccatt cttggcgcgc tggatatcga aggaagctat gataagcaac gaacgataac
    65641 tattgcgacc actccctcgg tcggagcctt ggtccttcct gtcatctatc gggcgattaa
    65701 aactcactat ccgcagcttt tactgcgcaa cccgcccatc agcgacgcgg aaaaccaact
    65761 cagtcagttt caaaccgatc tcatcatcga taacatgttt tgcaccaatc gtacggtgca
    65821 acatcatgtt ctgttcaccg acaatatggt gttaatttgc cgcaagggaa atccactact
    65881 ctctttagaa gatgacagag agactatcga caacgctgcg catgtacttc tgttaccgga
    65941 agggcaaaat ttcagcggtc tgcggcagag agttcaagag atgtttccgg accggcaaat
    66001 caatttcacc agctacaaca ttttgacaat cgctgcactg gttgccaaca gtgacatgtt
    66061 agcgattatt cccagccgtt tttataacct gtttagtcgt tgctggccgc tggaaaaact
    66121 gccttttccg tccttaaatg aggagcaaat agatttctct atccactaca acaaattcat
    66181 cctgcgtgat cccatcctgc acggagtaat agatgttatc cgtaatgcat tttgaaaaag
    66241 tattgttaca gccagatcac agcgcagaac gataaaatgg cacaatccaa caacaatttt
    66301 acattttcgc gaccgctttg gctgctttca ggtccgtttc aatgatatac tgccagtcgt
    66361 taattcaaaa atagttgata attacaacaa tctattgaat tgaaacgctt tccttcgtaa
    66421 ttcgcaactg gaacacgcac gctatgagta aacccattgt gatggaacgc ggtgttaaat
    66481 accgcgatgc cgataagatg gcccttatcc cggttaaaaa cgtggcaaca gagcgcgaag
    66541 ccctgctgcg caagccggaa tggatgaaaa tcaagcttcc agcggactct acacgtatcc
    66601 agggcatcaa agccgcaatg cgcaaaaatg gcctgcattc tgtctgcgag gaagcctcct
    66661 gccctaacct ggcggaatgc ttcaaccacg gcacagcaac gtttatgatc ctcggcgcta
    66721 tttgtacccg ccgttgtccg ttctgtgatg ttgcccacgg tcgcccggta gctcctgatg
    66781 ccaatgaacc agtgaaactg gcgcagacca ttgccgatat ggcgctgcgt tatgtggtta
    66841 tcacctccgt tgaccgtgat gacctgcgcg atggcggtgc ccaacacttt gcggattgta
    66901 ttactgccat tcgggaaaaa agcccgcaaa tcaaaattga aactctggtg ccggatttcc
    66961 gcggtcgaat ggatcgtgct ctggatattc tgactgcaac gcccccagat gtgttcaacc
    67021 ataacctgga aaacgtaccg cgtatttacc ggcaggtacg gcctggcgca gattacaact
    67081 ggtcgctgaa gctgctggaa cgctttaaag aagcgcatcc ggaaatcccg accaagtctg
    67141 gtctgatggt gggtctgggt gaaaccaatg aagaaattat tgaggtaatg cgcgacctgc
    67201 gccgtcatgg tgtgacgatg ttaacgctgg ggcaatattt gcagccaagc cgccatcacc
    67261 tgccggttca acgttacgtt agcccggatg agttcgacga aatgaaagcc gaagcgctgg
    67321 cgatgggctt tacccatgct gcatgcggtc cgtttgtccg ctcttcttac cacgccgatt
    67381 tgcaggcgaa aggaatggaa gttaagtaat cctgtaataa ttacttacat ctgtctgata
    67441 aaaaacccgc tttttgaagc gggttttttg tatcgaacaa gatattgagg gagcgtcctg
    67501 ctcgccacgt cactctttat gagagagctt ttcagcctga agatcaacgt ctgcgccttt
    67561 tttcgccgca gcatcgtcat cattcatcgc cttcttgaac cctttaatgg ccgctccaag
    67621 gtctccgccc agcgtacgta acttcttagt cccaaacagc agaacgacca gcgccgcaac
    67681 taccagcagt ttggtaatac taatctcacc catagatacc ttcttgacat ataaccggca
    67741 acagaacgcg ccttaaaaac cagagaatat taacggtcat tcgacgcgcg cacacaatag
    67801 caatctgtaa caaggtgaat caagcgccga gtaaaaaaac atcataataa ttgcggcggc
    67861 gcaaagcgac ggttgtttaa gatgggcagt tgcgcgcgca cctgacgcac acgttcgggc
    67921 gtcacttccg ccataatgag tgcaggcatt tctgacgctg ccgcaatggt gacgccaaag
    67981 ggatctataa tccggctttg cccgatattt ttgttcccgc actcccccgc cgccaccata
    68041 taacaggtgg tatccagcgc acgagcggca agcaacgttg accagtgatg ctctttgagc
    68101 ggcccacgaa cccaggcggc aggaagtacc aggatttcag ctccctgtaa agcctgtgcc
    68161 agcgccagct ctggaaagcg taagtcataa caggtcatca gaccgacctt catcccttcc
    68221 acctccagta acggagctat ttcattacca gcatcaacat ggcgtgattc ctgaatggca
    68281 aatgcatcat agagatgcag tttggcataa cgggcgacga tgtttcctgc ctgaagtgcc
    68341 accagcatat tccatgcgcg ccccggcgtt gaaggaagat gaatcgtcag aattgtcgtc
    68401 atcatgttac gtttactttc tcgccgtaaa cgtccgagga attcgccttc cagcagctgt
    68461 gctgatttaa ccgatagatc tgcatcatga tcatcgcgcg ccagcaatgc ttccggcagg
    68521 acaaacagcg atacgtcgtt ttccgccgcc tgcgccatca acgaggcaca aatctcagcg
    68581 ttcttttccc acacagatgt aacagcaaac tgtcctgcag caaccagcat tgccacctcc
    68641 tgtcagagtc gtaaaattcg ccaacgacga ttattcggcg ttacacttat cactcataca
    68701 aatcaaatag caggattttg cagtgttaca acttctttta gcagttttta ttggcggtgg
    68761 tacgggaagc gtagcgagat ggctgttaag tatgcgattt aacccgctgc atcaggcgat
    68821 tccgttgggg acgctggcag caaacctgat tggggcattc atcataggaa tgggattcgc
    68881 ctggttcagc aggatgacga acattgatcc agtgtggaaa gtattaatca ccaccggatt
    68941 ttgtggcggt ctaacaacct tctcaacatt ttcggcagaa gtggtgtttt tgttacaaga
    69001 gggccgcttt ggctgggcat tactgaacgt tttcgtcaac cttctggggt cttttgccat
    69061 gaccgcactg gcattctggc tgttttcggc ctcaaccgta cactaaagac caaaaaaaac
    69121 ccgctgatta agcgggtttt gaattcttgc tgacgtatct tacagagcga ttacgtttgc
    69181 agcagaaggg cctttggcac cgttagtgat ttcgaactct acgcgctgac cttcagcaag
    69241 agttttaaaa ccattagtct ggattgcaga gaagtgtacg aacacgtctt tgctgccgtc
    69301 ttccggagta atgaaaccga atcctttgga ctcattaaac cacttaacgt tacctttaat
    69361 cttagacatc aaaattacct ttacatgaaa aatagacaca aatgctgtgt cggttatcag
    69421 tacaccaatt gtggtacgct ttgtccagtg caactttact aaaaagtgat aaaagtcgcg
    69481 ttatttttga tgttgcttat tttagttatt gattttaaag aagttactaa aacttcattt
    69541 gtctcaaaac tgaaagcgca tccaggcaaa gtacacattg ccattgttgt aggtacccgg
    69601 aatgtaggtc atctgaaaag tcactgggcc ataacccacg gaggccaatg gcagtagaac
    69661 cgggagaggg atgtaattcc agttatcgcg tgccgttacg ccagcggtga atcccagacc
    69721 taaatgaaaa ttttcatccg ccagcggtcg ccaggtactt tcccatccgt atccggcaat
    69781 cggttcccat ttgttccacg agtccttaaa tgccatggca tacaggccat gccagtttcc
    69841 tttttcatcc caacgcgaca ggccaaaacc gccaccccac ggtcgctcgt tatagcgatc
    69901 ggttttttct ttgtcgtaag cgaaacgtgc atgccaggtg atggcaggaa tatataaatc
    69961 ataatgttca ggctgttgcc aggtttgtgc aatattttct ctaaacgttg tcatcctctc
    70021 atctgcgtta gcaaaaactt taccaacgct gattaactga ataaaaacaa aggaaaagat
    70081 agcgacatat ttactcacgt tcatttgtga ccataaaaca tttatcaaaa atctactact
    70141 agcatagcaa agctacaatt aacataacct taatagaccc aacataaaga ataatctgaa
    70201 tagctgctct atttatttcc gcttgttcaa gttaaatatt tccgcgcatc gattaaagat
    70261 gatgcgtcgt tttctgcgat gggaatagtc aaaaaagaaa aaccaagtct tttttgatga
    70321 caaaatgcaa tcaaggaaaa attattttat ttttttaacg agatacaaca atcatcttaa
    70381 cgaagtatat aatatatatc gtcaacccat gctcatatta gattattttt tgagcgcaca
    70441 gccgcagcta acaccattgc aattaacaaa tttgcatcaa tccaccatca atttacacac
    70501 attattatgt gataattgcc aaccgctaaa tatgcgtttt gttatctatg tataaaaaca
    70561 gcaacttcaa tgtcttaatg gcagtttttc ttgattttaa tcagcattca tcgccaattt
    70621 attgggcata ttttttcctt aagctttagg aagtttttat tatttacttt gggcctggag
    70681 acaggaaaaa ttatgctgac attcattgaa ctccttattg gggttgtggt tattgtgggt
    70741 gtggctcgct acatcattaa agggtattcc gccactggtg tgttatttgt cggtggcctg
    70801 ttattgctga ttatcagtgc cattatgggg cacaaagtgt taccgtccag ccaggcttca
    70861 acaggctaca gcgccacgga tatcgttgaa tacgttaaaa tattactaat gagccgcggc
    70921 ggcgacctcg gcatgatgat tatgatgctg tgtggctttg ccgcttacat gacccatatc
    70981 ggcgcgaatg atatggtggt caagctggcg tcaaaaccat tgcagtatat taactcccct
    71041 tacctgctga tgattgccgc ctattttgtt gcttgtctga tgtcactggc cgtctcttcc
    71101 gcaaccggtc tgggggtttt gctgatggca accctgtttc cggtgatggt aaacgttggt
    71161 atcagtcgtg gcgcagcagc tgccatttgt gcctccccgg cggcgattat tctcgcaccg
    71221 acttcagggg atgtggtgct ggcggcgcag gcttccgaaa tgtcgctgat tgacttcgcc
    71281 ttcaaaacga cgctgcctat ctcaattgct gcaattatcg gcatggcgat cgcccacttc
    71341 ttctggcaac gttatctgga taaaaaagag cacatctctc atgaaatgtt agatgtcagt
    71401 gaaatcacca ccactgcccc cgcgttttat gccattttgc cgttcacgcc gatcatcggt
    71461 gtactgattt ttgacggtaa atggggtccg caattacaca tcatcactat tctggtgatt
    71521 tgtatgctga ttgcctccat tctggagttc ctccgcagct ttaataccca gaaagttttc
    71581 tctggtctgg aagtggctta tcgcgggatg gcagatgcgt ttgctaacgt ggtgatgctg
    71641 ctggttgccg ctggggtatt cgctcagggg cttagcacca tcggctttat tcaaagtctg
    71701 atttctatcg ctacctcgtt tggttcggcg agtatcatcc tgatgctggt attggtgatt
    71761 ctgaccatgc tggcggcagt cacgaccggt tcaggcaatg cgccgtttta tgcgtttgtt
    71821 gagatgatcc cgaaactggc gcactcttcc ggcattaacc cggcgtattt gactattccg
    71881 atgctgcagg cgtcaaacct gggtcgtacc ctatcacccg tttctggcgt agtcgttgcg
    71941 gttgccggga tggcgaaaat ctcaccattt gaagtcgtaa aacgcacctc ggtgccggtg
    72001 cttgttgggc tagtgattgt tatcgttgct acagagctga tggtgccagg aacggcagca
    72061 gcggtcacag gcaagtaaat agtaatgccg gaggcaagtt tcctccggct ttatcatgtc
    72121 acccactgtg gtatatgcgt tgtggtctgc caactttgcc gtgaacaatt tcagcaataa
    72181 tcagatggcg gctggcgcaa tattcaagat aacgcctggc agtggtgcgg ctgatggtca
    72241 gtgcctgcgc caccgtttct gccgtatgtt gcacaccagg ctctttaaac agttttcgca
    72301 ccgcgtttag cgtcaagggg tcaatgccgg tcggcagttc gtctttgggt tcaccgcgag
    72361 cataggcatt aaacatctca tcaatttgct tctggctggc gctatcaata ctttccagca
    72421 tatgtttacg ctggcggaaa cgggttagcg tttgccccag tgtattgatt cacttgaagt
    72481 acgaaaaaac cgggagaaca ttggattatt cgggatctga tgggattaga tttggtgggg
    72541 cttgcaagcc tgtagtacaa attttaggcg ttaaccaatg aaacgcgaaa gatagtaaaa
    72601 aattgctttt gtttcattga aaaaacgaaa aacaaaaaca ctataaatca tttcaatcat
    72661 agcttcaaaa aacgttcaaa accgataaca accaggctgt catcaaatga ctcatatcac
    72721 aaatcagctc atgccgttta ggtatgttac atgtgtgatt atgtgaggtg aagtatgttt
    72781 tagctggctc atggttgtta tacggctttt ttcgcatctt gtggttccaa caaaggagct
    72841 acaggctctt cctgtttatg aacaccatac attgacaaaa tctcttggcg agtttctgga
    72901 ggtaatttat gcagcgccaa agctgtttgt agtgtttggc gatcaatacc cagcgttgcc
    72961 aatagggtgt caacggagat ggaggggcgt tcttgcatta atccggaaag cccatattta
    73021 gccatgttta gctctatctc tcggacttca ttttctccta gtttattgat taacagggag
    73081 aacattttca atgtatcagc attcatttct tcgatagcaa ttttttgcat agccggtatt
    73141 gccgggatat attcaacccc aggcccgcga cgcccttctt gtgaacgctt cggccatcct
    73201 tctttctttg ctcgtttatg caccccctgt attgtcccag gcattccatc cagttcagta
    73261 atttgtttgg gtgtcatcca ctggtttatc tcatttgcgc ttttcataaa ctaagtttac
    73321 cagatttatc tatataaaaa tcaatggatt acaaaataaa ccacattaac tgatataaac
    73381 cggtttggtt tatttggtaa tgtataaaac agataatcaa gacgaattat ttgttttggt
    73441 aaccctttat catacgcaat atggttggtg tgcgaaagat gagtaacgat tggcatcggg
    73501 ccgacgtgat tgcggccctc aaaaaaacag gaacccctct ggcaaaattg tccagacgtt
    73561 caggttattc aaccgggacg cttgcaaatg cattagtcag gccatggcct aaaggtgaac
    73621 gcattattgc tgacgcctta aacatgaaac ctgaggaaat ttggcctagt agatatctaa
    73681 gggatcacaa tgagagagtg gatatcagca attgaatgca tgggtttatc tggtttacct
    73741 ggttcattaa tgggggttca caaaagagct cagagagaag gatggctcaa gagaagaaga
    73801 gaaggaatta gaggggttgc ttacgaatac catatatctt ctcttcctaa cattgctcga
    73861 gctgaagttt tgctccaaca aggaaaattt aaaaccagtc agggatgttt tgaaatcgcc
    73921 cgccccacgc tggaagctca tgattatgat cgtgaggcac tatggagcaa atgggataaa
    73981 gccagcgatt cccagcgcag cctggctgaa aaatggttgc cctcaattca ggccacagac
    74041 gaaatgctga accaggggat ttcaacgaaa acggcttttg cgaccgttgc agggcattac
    74101 caggtcagcg catccacttt gcgggacaag tattaccagg tacagaagtt tgcgaagcct
    74161 gactgggcgg ctgcacttgt tgatggacgt ggagcatccc gtcgcaatgt tcacaaaagt
    74221 gaatttgacg aggatgcctg gcagtttctg attgcagatt atctgcgacc ggaaaaaccc
    74281 gccttccgca aatgttatga gcgtctggaa ctggcagccc gcgagcatgg ctggagtatt
    74341 ccctcccgtg ccacggcctt tcgccggatt cagcaactgg acgaggcaat ggttgttgcc
    74401 tgtcgtgaag gtgaacatgc actgatgcat ctgataccgg cacagcagcg aactgtggaa
    74461 cacctggacg ccatgcagtg gatcaacggc gacggttatc tgcataacgt ctttgtacgc
    74521 tggtttaacg gtgatgtgat ccgtccgaaa acatggttct ggcaggatgt gaaaacccga
    74581 aaaattctgg gctggcgctg cgatgtgagc gagaacattg attcaattcg cctctcgttc
    74641 atggatgttg tgactcgcta cagtatcccg gaggattttc acatcaccat tgataacacc
    74701 cgtggtgctg cgaataaatg gctgacggga ggcgcgccca atcgctaccg ctttaaggta
    74761 aaagaggacg atccaaaagg actgttttta ctgatgggtg cgaaaatgca ctggacaagc
    74821 gttgttgccg gtaaaggctg gggccaggca aaacctgttg aacgtgcttt cggtgttggt
    74881 gggcttgagg aatacgttga taagcatccg gcactggctg gcgcatatac ggggccaaat
    74941 ccgcaggcaa aacctgataa ctatggcgac cacgctgttg atgcagagct gtttctgaaa
    75001 acctttgccg aaggtgtggc gatgttcaat gccagaacag gccgtgaaac agaaatgtgc
    75061 gggggcaaac tctcgtttga tgatgttttc gagcgtgaat acgccagaac gattgtgcgt
    75121 aagccaaccg aagaacaaaa acggatgctg ttactgcctg ccgaggcggt gaacgtttca
    75181 cgcaaaggcg agtttacgct taaagttggc ggctccctta aaggcgcgaa aaacgtttat
    75241 tacaacatgg cattaatgaa tgccggagtg aaaaaagttg tggtcagatt tgatccacag
    75301 cagttacaca gcacggttta ttgagatagc ctgagtttaa cggacactcc ttcctgaaat
//
LOCUS       DAJTQQ010000004        72967 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000004 DAJTQQ010000000
VERSION     DAJTQQ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72967
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.004"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO309_000315"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO309_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5845591.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO309_000316"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO309_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR5845592.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO309_000317"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO309_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR5845593.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO309_000318"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO309_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR5845594.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO309_000319"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO309_000319"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR5845595.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO309_000320"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO309_000320"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR5845596.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO309_000321"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO309_000321"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR5845597.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OO309_000322"
     CDS             complement(4598..5320)
                     /locus_tag="OO309_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5845598.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO309_000323"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO309_000323"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5845599.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OO309_000324"
     CDS             6520..6738
                     /locus_tag="OO309_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR5845600.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO309_000325"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO309_000325"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5845601.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OO309_000326"
     CDS             complement(7472..7672)
                     /locus_tag="OO309_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR5845602.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO309_000327"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO309_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO309_000328"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO309_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR5845603.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="OO309_000329"
     CDS             10101..12014
                     /locus_tag="OO309_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5845604.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSSEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="OO309_000330"
     CDS             12014..13036
                     /locus_tag="OO309_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5845605.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="OO309_000331"
     CDS             13030..13248
                     /locus_tag="OO309_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR5845606.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="OO309_000332"
     CDS             13302..14171
                     /locus_tag="OO309_000332"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR5845607.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="OO309_000333"
     CDS             complement(14226..14630)
                     /locus_tag="OO309_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR5845608.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="OO309_000334"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="OO309_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR5845609.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="OO309_000335"
     CDS             15615..17705
                     /locus_tag="OO309_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR5845610.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OO309_000336"
     CDS             complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OO309_000336"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR5845611.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OO309_000337"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OO309_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR5845612.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="OO309_000338"
     CDS             complement(19673..20275)
                     /locus_tag="OO309_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR5845613.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="OO309_000339"
     CDS             complement(20265..20432)
                     /locus_tag="OO309_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR5845614.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OO309_000340"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OO309_000340"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR5845615.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22570
                     /gene="tsgA"
                     /locus_tag="OO309_000341"
     CDS             21389..22570
                     /gene="tsgA"
                     /locus_tag="OO309_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR5845616.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22832..25375
                     /gene="nirB"
                     /locus_tag="OO309_000342"
     CDS             22832..25375
                     /gene="nirB"
                     /locus_tag="OO309_000342"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR5845617.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25372..25698
                     /gene="nirD"
                     /locus_tag="OO309_000343"
     CDS             25372..25698
                     /gene="nirD"
                     /locus_tag="OO309_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR5845618.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25824..26630
                     /gene="nirC"
                     /locus_tag="OO309_000344"
     CDS             25824..26630
                     /gene="nirC"
                     /locus_tag="OO309_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR5845619.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26649..28022
                     /gene="cobA"
                     /locus_tag="OO309_000345"
     CDS             26649..28022
                     /gene="cobA"
                     /locus_tag="OO309_000345"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5845620.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28269..28436
                     /locus_tag="OO309_000346"
     CDS             28269..28436
                     /locus_tag="OO309_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR5845621.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28731..30068
                     /gene="frlA"
                     /locus_tag="OO309_000347"
                     /pseudo
     CDS             28731..30068
                     /gene="frlA"
                     /locus_tag="OO309_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30089..31111
                     /gene="frlB"
                     /locus_tag="OO309_000348"
     CDS             30089..31111
                     /gene="frlB"
                     /locus_tag="OO309_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR5845622.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31161..31991
                     /gene="frlC"
                     /locus_tag="OO309_000349"
     CDS             31161..31991
                     /gene="frlC"
                     /locus_tag="OO309_000349"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR5845623.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31988..32773
                     /gene="frlD"
                     /locus_tag="OO309_000350"
     CDS             31988..32773
                     /gene="frlD"
                     /locus_tag="OO309_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR5845624.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32873..33604
                     /gene="phoB"
                     /locus_tag="OO309_000351"
     CDS             32873..33604
                     /gene="phoB"
                     /locus_tag="OO309_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR5845625.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33764..34849)
                     /locus_tag="OO309_000352"
     CDS             complement(33764..34849)
                     /locus_tag="OO309_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5845626.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34861..36165)
                     /locus_tag="OO309_000353"
     CDS             complement(34861..36165)
                     /locus_tag="OO309_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR5845627.1"
                     /translation="MDLYIQIIVVACLTGMTSLLEHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36177..36530)
                     /locus_tag="OO309_000354"
     CDS             complement(36177..36530)
                     /locus_tag="OO309_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR5845628.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36541..37419)
                     /locus_tag="OO309_000355"
     CDS             complement(36541..37419)
                     /locus_tag="OO309_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR5845629.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37416..38642)
                     /locus_tag="OO309_000356"
     CDS             complement(37416..38642)
                     /locus_tag="OO309_000356"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5845630.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38643..39806)
                     /locus_tag="OO309_000357"
     CDS             complement(38643..39806)
                     /locus_tag="OO309_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR5845631.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39890..40252)
                     /locus_tag="OO309_000358"
     CDS             complement(39890..40252)
                     /locus_tag="OO309_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR5845632.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40269..41174)
                     /locus_tag="OO309_000359"
     CDS             complement(40269..41174)
                     /locus_tag="OO309_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5845633.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="OO309_000360"
     CDS             complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="OO309_000360"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR5845634.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42441..43199)
                     /gene="gph"
                     /locus_tag="OO309_000361"
     CDS             complement(42441..43199)
                     /gene="gph"
                     /locus_tag="OO309_000361"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR5845635.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="OO309_000362"
     CDS             complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="OO309_000362"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR5845636.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43887..44723)
                     /gene="dam"
                     /locus_tag="OO309_000363"
     CDS             complement(43887..44723)
                     /gene="dam"
                     /locus_tag="OO309_000363"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5845637.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44830..46122)
                     /gene="damX"
                     /locus_tag="OO309_000364"
     CDS             complement(44830..46122)
                     /gene="damX"
                     /locus_tag="OO309_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR5845638.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46214..47302)
                     /gene="aroB"
                     /locus_tag="OO309_000365"
     CDS             complement(46214..47302)
                     /gene="aroB"
                     /locus_tag="OO309_000365"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR5845639.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47359..47880)
                     /gene="aroK"
                     /locus_tag="OO309_000366"
     CDS             complement(47359..47880)
                     /gene="aroK"
                     /locus_tag="OO309_000366"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR5845640.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48281..49519)
                     /gene="hofQ"
                     /locus_tag="OO309_000367"
     CDS             complement(48281..49519)
                     /gene="hofQ"
                     /locus_tag="OO309_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR5845641.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49431..49837)
                     /gene="hofP"
                     /locus_tag="OO309_000368"
                     /pseudo
     CDS             complement(49431..49837)
                     /gene="hofP"
                     /locus_tag="OO309_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            complement(49827..50267)
                     /gene="hofO"
                     /locus_tag="OO309_000369"
     CDS             complement(49827..50267)
                     /gene="hofO"
                     /locus_tag="OO309_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR5845642.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50251..50790)
                     /gene="hofN"
                     /locus_tag="OO309_000370"
     CDS             complement(50251..50790)
                     /gene="hofN"
                     /locus_tag="OO309_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR5845643.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50790..51569)
                     /gene="hofM"
                     /locus_tag="OO309_000371"
     CDS             complement(50790..51569)
                     /gene="hofM"
                     /locus_tag="OO309_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR5845644.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51689..54241
                     /gene="mrcA"
                     /locus_tag="OO309_000372"
     CDS             51689..54241
                     /gene="mrcA"
                     /locus_tag="OO309_000372"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR5845645.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54406..54966)
                     /gene="nudE"
                     /locus_tag="OO309_000373"
     CDS             complement(54406..54966)
                     /gene="nudE"
                     /locus_tag="OO309_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR5845646.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55286..57421
                     /gene="igaA"
                     /locus_tag="OO309_000374"
     CDS             55286..57421
                     /gene="igaA"
                     /locus_tag="OO309_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR5845647.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57486..58154
                     /gene="yrfG"
                     /locus_tag="OO309_000375"
     CDS             57486..58154
                     /gene="yrfG"
                     /locus_tag="OO309_000375"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR5845648.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58165..58566
                     /gene="hslR"
                     /locus_tag="OO309_000376"
     CDS             58165..58566
                     /gene="hslR"
                     /locus_tag="OO309_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR5845649.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58591..59460
                     /gene="hslO"
                     /locus_tag="OO309_000377"
     CDS             58591..59460
                     /gene="hslO"
                     /locus_tag="OO309_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR5845650.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59604..61355)
                     /locus_tag="OO309_000378"
     CDS             complement(59604..61355)
                     /locus_tag="OO309_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR5845651.1"
                     /translation="MDNVELSPATRWGMIATGLLQGMIATGLLQGLVCYLLIAWLAGK
                     NHSWIVYGVPATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNP
                     WRAEKAIWDFGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLS
                     NGLTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDS
                     IQKLFTLIATGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAI
                     VRDPQKASLPWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAEL
                     VLLVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSH
                     MARYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQH
                     LQEQVSEKVFAENVLIAPGSVKPDATFWSALIQGRYNVMTCIEKDACVLVEQDLNSDG
                     QAERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKA
                     WRDLTVDGETLEINLSK"
     gene            61734..63356
                     /gene="pckA"
                     /locus_tag="OO309_000379"
     CDS             61734..63356
                     /gene="pckA"
                     /locus_tag="OO309_000379"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR5845652.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63432..64784)
                     /gene="envZ"
                     /locus_tag="OO309_000380"
     CDS             complement(63432..64784)
                     /gene="envZ"
                     /locus_tag="OO309_000380"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR5845653.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64781..65500)
                     /gene="ompR"
                     /locus_tag="OO309_000381"
     CDS             complement(64781..65500)
                     /gene="ompR"
                     /locus_tag="OO309_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR5845654.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65728..66204
                     /gene="greB"
                     /locus_tag="OO309_000382"
     CDS             65728..66204
                     /gene="greB"
                     /locus_tag="OO309_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR5845655.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66301..68622
                     /locus_tag="OO309_000383"
     CDS             66301..68622
                     /locus_tag="OO309_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR5845656.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDATLLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69060..69287
                     /gene="feoA"
                     /locus_tag="OO309_000384"
     CDS             69060..69287
                     /gene="feoA"
                     /locus_tag="OO309_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR5845657.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69304..71625
                     /gene="feoB"
                     /locus_tag="OO309_000385"
     CDS             69304..71625
                     /gene="feoB"
                     /locus_tag="OO309_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR5845658.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71625..71861
                     /gene="feoC"
                     /locus_tag="OO309_000386"
     CDS             71625..71861
                     /gene="feoC"
                     /locus_tag="OO309_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR5845659.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72064..72951
                     /gene="rpnA"
                     /locus_tag="OO309_000387"
     CDS             72064..72951
                     /gene="rpnA"
                     /locus_tag="OO309_000387"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR5845660.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASRLAP"
BASE COUNT        17587 a        19775 c        18454 g        17151 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaaga gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcagt gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattacacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacagatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa agaaaagtgg gggatcggcg tactgtttct
    22021 ctctgttgcg gcgctgtgct acatcctcgg tcagttaggt tttatctcct gggtgcctga
    22081 gtatgccaaa ggcctgggca tgagcctgaa cgacgcgggc acgctggtga gtaacttctg
    22141 gatgtcatac atggtcggca tgtgggcgtt cagctttatt cttcgcttct ttgatttgca
    22201 acgcattctg accgtactgg ctggtctggc tgcgattctg atgtacgtct ttaacaccgg
    22261 aacaccagta catatggcgt ggtcaattct cgctctgggc ttcttctcca gcgcgatcta
    22321 taccaccatc atcaccctgg gttcacagca gaccaaagta ccgtcgccaa aactggttaa
    22381 ctttgtcctg acctgcggaa ccatcggtac tatgttgacc tttgtggtta ccggcacgat
    22441 tgttgaacat agcggtccgc aggcggcact gcttacagca aacggtctgt acgctgtcgt
    22501 ctttgtgatg tgcttcctgt taggtttcgt cagccgtcac cgtcagcata acaccctgac
    22561 ctctcattaa ttgctaatgc cggacggcac tatcgtcgtc cggccttttc ctctcttccc
    22621 ccgctacgtg catctatttc tataaacccg ttcattttgt ccattttttg cacaaacatg
    22681 aaatgtcaga caattccgtg acttaagaaa atttatacaa atcagcaata taccccttaa
    22741 ggagtatata aaggtgaatt tgatttacat caataagcgg ggttgctgaa tcgttaaggt
    22801 aggcggtaat agaaaagaaa tcgaggcaaa aatgagcaaa gtcagactcg caattatcgg
    22861 taacggtatg gtcggccatc gctttatcga agatcttctt gataaatctg atgcggccaa
    22921 ctttgatatt accgttttct gtgaagaacc gcgcatcgct tatgaccgcg tacacctctc
    22981 atcttacttc tctcaccaca ccgccgaaga gctgtcgctg gtgcgcgaag gcttctacga
    23041 gaaacacggc atcaaagttc tggtcggcga acgcgctatc accatcaacc gtcaggagaa
    23101 ggtgattcac tccagcgccg gacgtaccgt tttttatgac aagctgatca tggcaaccgg
    23161 ttcctacccg tggatcccac caatcaaagg ttctgatact caggactgct ttgtctatcg
    23221 caccattgaa gacctcaacg ccattgaatc ctgcgcccgt cgcagcaaac gcggtgccgt
    23281 tgttggtggc ggcctgttag gtctggaagc cgctggcgcg ctgaaaaact taggtattga
    23341 aacccacgtt atcgaatttg cccctatgct gatggcggaa cagcttgatc agatgggtgg
    23401 cgagcagctg cgtcgcaaaa tcgaaagtat gggcgtgcgc gttcacacca gcaaaaacac
    23461 ccttgagatt gtgcaggaag gtgtcgaagc gcgtaaaacc atgcgttttg ccgacggcag
    23521 cgaactggaa gtcgacttta tcgtcttctc taccggtatc cgtccgcgcg ataagctggc
    23581 aacccagtgt ggtctggacg ttgctccgcg tggggggatt gtcattaatg attcctgcca
    23641 gacttccgat ccggatatct acgccatcgg tgaatgcgca agctggaaca accgtgtatt
    23701 tggtctggta gcaccaggct acaaaatggc gcaggtcgcc gttgaccata ttctgggtag
    23761 cgaaaacgcc tttgaaggtg ctgaccttag cgccaagctg aaactgctgg gcgtagacgt
    23821 aggtggtatt ggtgatgcgc acggtcgcac gcctggcgca cgtagctatg tttacctcga
    23881 cgaaagtaaa gagatctaca aacgcctgat tgtcagcgaa gacaacaaaa ccctgctcgg
    23941 tgcggtactg gtgggcgata ccagcgacta cggcaacctg ctgcaactgg tgctgaacgc
    24001 tatcgagctg ccggaaaacc cggattctct gattctgccg gcgcactcgg gtagcggtaa
    24061 gccgtctatc ggtgttgata aactgccgga cagcgcgcaa atctgctcct gcttcgacgt
    24121 cactaaaggt gatctgattg ctgccatcaa caaaggctgc cacacagttg cggcgctgaa
    24181 agctgaaacc aaagcgggta ctggctgcgg tggctgtatc ccgctggtca ctcaggtact
    24241 gaacgcggaa ctggcgaaac agggcatcga agttaacaac aacctgtgcg aacactttgc
    24301 ttattcgcgt caggaactgt tccatttgat ccgcgttgaa ggcattaaaa ccttcgaaga
    24361 actgctggcg aaacacggca aaggctacgg ttgtgaagtt tgtaaaccaa ccgtcggttc
    24421 gctgctggcc tcctgctgga acgaatacat tctgaagccg gaacatactc cgctgcagga
    24481 ttctaacgac aacttcctcg ctaacatcca gaaagacggc acctactcgg tgatcccgcg
    24541 ttctccgggc ggtgaaatca ccccggaagg gctgatggca gtgggtcgta tcgcgcgtga
    24601 atttaatctc tacaccaaaa tcaccggctc ccagcgcctg gcgatgtttg gcgcacagaa
    24661 agacgatctg ccggaaatct ggcgtcagct gattgaagct ggcttcgaaa ccggtcatgc
    24721 ctatgcgaaa gcactgcgta tggcgaaaac ctgcgtgggt agcacctggt gccgctacgg
    24781 tgttggcgat agcgtcggcc tcggcgtgga actggaaaac cgctacaaag gcatccgtac
    24841 gccgcacaaa atgaagttcg gtgtctccgg ctgtacccgt gaatgttcag aagctcaggg
    24901 taaagacgtg ggtattatcg ccactgaaaa aggctggaac ctgtatgttt gcggtaacgg
    24961 cggcatgaaa ccgcgtcatg cggatctgct ggcagcggat atcgatcgcg aaacgctgat
    25021 caaatatctc gaccgcttca tgatgttcta catccgtacc gccgacaaac tgacgcgtac
    25081 cgcgccgtgg ttagaaaacc tcgaaggcgg catcgattac ctgaaagcgg tgatcattga
    25141 cgacaaactg gggctgaacg ctcatctgga agaagagatg gcgcgcctgc gtgaagcggt
    25201 agtgtgtgag tggactgaaa cggtcaatac accgtccgcg cagactcgct tcaaacactt
    25261 catcaacagc gacaagcgtg acccgaacgt gcagatggtg ccagagcgcg aacagcaccg
    25321 tccggcaacg ccgtatgaac gtatcccggt aactctggtg gaggacaacg catgagccag
    25381 tggaaagaca tctgcaaaat cgatgacatc ctgcctgaaa ccggcgtctg cgcactgtta
    25441 ggtgacaagc aggtagcgat tttccgcccg tatcacagcg accaggtgtt tgcgatcagc
    25501 aacattgatc cgttcttcga gtccagcgtg ctatcacgcg ggctgattgc ggaacaccag
    25561 ggcgagctgt gggtcgccag cccgctgaaa aaacagcgtt ttcgcttaag tgatggcttg
    25621 tgcatggaag acgaacagtt ttccgtcaaa cattacgaag cacgagtgaa agacggcgtg
    25681 gtgcagctgc gcggttaatg ttttaacggg aggcgcaatg cctccccttt ttgcatggtc
    25741 ctgtaatgac cttcggtata ttgcaggaca ttttttaaac tttttgtttt attttttgtt
    25801 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaatg
    25861 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25921 tggcgggcgc atatgtgggt cttgggatca tcctgatttt cacgctcggt aatttgctcg
    25981 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26041 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26101 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26161 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26221 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26281 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct ggcaatctgg atggcgctgc
    26341 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26401 ccggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26461 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26521 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26581 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacagc tgccggttaa ttactaaggg
    26641 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26701 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26761 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26821 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26881 cagcgacgga tgatgacacg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    26941 tcttctgtaa cgtagttgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27001 accgctcacc gctaatggta gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc
    27061 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27121 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt
    27181 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27241 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27301 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27361 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27421 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27481 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27541 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27601 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27661 cctattcggg tattccactc acgcatcgcg attatgccca gagcgtacgc ttaattaccg
    27721 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27781 tggtgttcta tatggggttg aatcaggccg cgactattca gcaaaagctg attgaacacg
    27841 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacggcagtc acgcagcgcg
    27901 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    27961 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaatcatt
    28021 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28081 ataaaacata accccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28141 atttacaaaa ttaagtcatt cgctcactga cgccaattaa atattcctgt atcgttctta
    28201 acgagcagtc tgcttatatt catataatca atgaatatta attaatatat aatacatagg
    28261 gaatgtaaat gaacaaattt attaaagttg cactggtagg tgcagtactg gctacgttaa
    28321 ctgcatgtac tggtcatatt gaaaaccgtg ataagaactg ctcttacgac tacctgctgc
    28381 acccggcaat ttctatttct aaaatcattg gcggttgcgg tcctactgca cagtaagtct
    28441 ctgacagaaa tcggctaaca ccggtttaat tgcccgacgc cctctggatt ttccgagggc
    28501 gtatttgttt ctatgtcctt cttgcccccg cccgtaaaaa tattttcact ttaaattcaa
    28561 ttttataact acatccatta gccacagcgc gatcctgtgc gaaattttgt gatcttcctc
    28621 cacatcacat aacataatat gttgttatat tcatcatgca ttgtcatgtt accttttaaa
    28681 tggctgcaaa ctctccccta caacgtattg gacaagaaaa aggtatcgct atgggaagcc
    28741 aggaactcca acgcaagctc ggattttggg ccgttcttgc aatcgccgtc gggacaaccg
    28801 tcggctccgg tatttttgta tctgtgggtg aagtggcaaa agcagcgggc acgccgtggc
    28861 ttacggtgct cgcgtttgtc attggcgggt taattgtgat cccgcaaatg tgcgtctatg
    28921 cggaactatc caccgcttat ccggaaaatg gcgcagatta tgtttatctg aaaaatgccg
    28981 gaagccgacc gctggctttc ctctccggct gggccagctt ctgggccaac gatgcgccgt
    29041 cattgtcgat tatggcgctg gcgattgtca gcaatcttgg ctttttaacg cctatcgatc
    29101 cgttgctcgg taaatttatc gccgccggat taattatcgc ctttatgttg ctacacctgc
    29161 gctccgttga aggcggcgca gcgtttcaga cgctaattac catcgccaaa attatcccgt
    29221 tcactatcgt cattggcctt gggatcttct ggtttaaagc ggagaatttt gccgccctta
    29281 ccaccactgc gattggcgca acgggcagct ttatggcgct gctggcgggg atctctgcca
    29341 ccagttggtc gtataccggc atggcctcta tctgttatat gaccggcgaa attaaaaacc
    29401 ccggaaaaac catgccacga gcgctgattg gttcctgtct gctggttctg gtgctctaca
    29461 ccctgctggc gctggtgatt tccggcctga tgcccttcga caaactcgcc aattctgaaa
    29521 cgccgatttc cgacgccctg acctagatcc ccgcactcgg cagcaccgct gggctctttg
    29581 ttgccatcac ggcgatgatc gtcattcttg gttcgctttc cagctgcgtg atgtaccagc
    29641 cgcggctgga atacgcgatg gcgaaagaca acctgttctt taaatgcttc ggccatgcgc
    29701 atccgaaata caacacgccg gatgtctcca tcatcctgca aggggcgctg gggatcttct
    29761 tcatcttcgt ttccgatctc accagcctgc tgggttattt caccctggtg atgtgtttca
    29821 aaaataccct caccttcggc tccatcatct ggtgtcgtaa acgcgacgat tacaaaccgc
    29881 tgtggcgtac tccggctttc gggctgatga ccaccctcgc cattgcgtca agcctcattc
    29941 tggtcgcctc aacctttgtc tgggcaccga ttcccggcct tatctgcgcc gccatcgtta
    30001 ttgctactgg tctgcctgct tacgccttct gggcgaagcg tagccgccag ctcaacgctt
    30061 tgtcgtaatg ttatctgaag aaaataaaat gttggatatt gataaaagca ccgtggactt
    30121 tctggtcacc gaaaatatgg ttcaggaagt ggaaaaagtt ctcagccatg acgttccgct
    30181 ggtgcacgcc atcgtggaag agatggtgaa gcgcgacatt gatcgtattt atttcgttgc
    30241 ctgcggctcg ccactcaacg cggcgcaaac ggcgaaacat ctggcggatc gcttttccga
    30301 tcttcaggtc tacgccattt ccggctggga gttctgcgat aacaccccgt atcgcctcga
    30361 cgatcgttgc gcggtgattg gcgtttctga ctacggtaaa acggaagagg taatcaaagc
    30421 gctggagctg ggccgggcct gcggagcact cactgcggcg ttcaccaaac gcgcggacag
    30481 cccgattacc tcggcggcgg aatttagcat tgattatcag gccgactgta tctgggaaat
    30541 tcacctgctg ctctgctaca gcgtggtgct ggagatgatc acccgcctcg cgccgaacgc
    30601 ggaaatcggc aagatcaaaa acgatctcaa gcagttgccg aatgcgctcg gtcatctggt
    30661 acgcacctgg gaagaaaaag gccgccagct tggtgaactg gccagccagt ggccgatgat
    30721 ttataccgtt gctgcgggtc cgctgcgtcc gctgggttac aaagaaggca ttgtaacgct
    30781 gatggaattt acctggacgc acggctgcgt gattgagagc ggagagttcc gccatggccc
    30841 gctggagatt gtcgaaccgg gcgttccgtt cctgttcctg ctcggcaatg atgaaagtcg
    30901 ccacaccacc gaacgcgcca ttaactttgt taaacagcgt actgacaacg tgatcgtcat
    30961 cgattacgcc gaaatttcgc aagggctgca cccgtggctg gcaccgttcc tgatgttcgt
    31021 gccaatggag tggctctgct actacctgtc tatttacaaa gatcacaacc cggatgaacg
    31081 ccgctattac ggtggtctgg tggaatatta atccctctcc cggcccggca acgggccgga
    31141 tttacgcaag gagttacccg atgaaaacag gtatgtttac ctgcggccac cagcggctgc
    31201 ctattgaaca cgcatttcgt gatgcaagcg agctgggtta cgacggcatc gaaatttggg
    31261 gcggtcgccc gcacgcgttc gcgccggact taaaagcggg cggcatcaaa caaatcaagg
    31321 cgctggcgca gacgtatcag atgccgatta tcggttacac gccagaaacc aacggctatc
    31381 cgtataacat gatgctgggc gatgaacata tgcgtcgtga aagcctggac atgatcaagc
    31441 tggcgataga tatggcaaaa gagatgaacg cgggttatac gctgatttct gcggcccacg
    31501 cgggctatct cacgccgcct aacgttatct gggggcggct ggcagagaac ttaagcgaac
    31561 tgtgtgagta cgcggaaaac atcggcatgg atctgatcct cgaaccgtta acgccgtatg
    31621 aatcgaacgt cgtgtgtaat gctaatgatg tgcttcatgc gctggcgctg gtgccttcgc
    31681 cgcgcctgtt cagcatggtc gacatttgcg cgccgtatgt ccaggcggaa ccggtgatga
    31741 gttatttcga caaactgggc gataaattac gtcatctgca tattgtcgac agcgacgggg
    31801 ccagcgacac gcattacatt cctggagaag gcaaaatgcc gctgcgggaa ctgatgcgcg
    31861 atattattga tcggggctat gagggttact ctacggtgga gctggtgacg atgtatatga
    31921 acgagcccag actctatgcc cgccaggcgc tggaacgctt tcgcgcgctg ctgccggagg
    31981 atgagagatg aaaaccctgg cgacaatcgg cgataactgc gtcgatatct acccgcaact
    32041 gaataaagcg ttttctggcg gtaatgcggt caatgtggcg gtgtactgca ctcgctacgg
    32101 catacagccg ggatgcatta cctgggtggg tgacgatgac tacggcacaa agctgaagca
    32161 ggatctcgcc cgcatgggcg tcgatatcag ccatgtccat acgaaacacg gcgttaccgc
    32221 acaaactcag gtggaactgc acgacaatga tcgcgttttt ggcgactaca ccgaaggcgt
    32281 gatggccgac tttgccctga gtgaagagga ttacgcctgg ctggcgcagt atgacattgt
    32341 gcacgcggca atctggggac atgcggaaga cgcattccca cagctgcacg ctgcgggcaa
    32401 acttaccgct ttcgacttct ccgacaagtg ggacagcccg ctctggcaga cactggtgcc
    32461 gcatctcgat tttgcctttg cctccgcacc gcaagaagac gaagcgctgc gtctgaagat
    32521 gaaagcgatt gttgcccgtg gcgcaggaac agtgattgtc acgctgggtg aaaacggcag
    32581 cattgcctgg gatggcgcgc agttctggcg tcaggctcct gaaccggtga cggttatcga
    32641 caccatgggt gccggagatt cgttcattgc cggattcctt tgcggctggt ctgcggggat
    32701 gacattaccg caggcgatgg cgcagggaac ggcgtgcgcg gcgaaaacca ttcagtacca
    32761 cggtgcctgg taggtataac gttggcgtga gcatcttcac gccaacgtgc tgttacttgc
    32821 cggaaaacga ccctataatc cgagtaattc attctttatt tcagggtcga ttatgtcagc
    32881 tacggaccgc tactctcatc aactcctcta cgctaccgtc cgccagcggc tgctggatga
    32941 tatcgcgcag ggggtttacc aggccgggca acagatccct accgaaaacg agctttgtac
    33001 acaatataac gtcagccgca ttaccattcg caaagccatc agcgacttag tggcagacgg
    33061 cgtactgatc cgctggcagg gaaaaggcac ctttgtacaa agccagaaag ttgaaaacgc
    33121 cctgcttact gtcagtggtt ttaccgattt tggcgtctca caaggcaagt cgacgaaaga
    33181 gaaagtgatc gaacaggaac gggtcagcgc cgcgccgttt tgcgaaaagc tgaacatccc
    33241 cggaaacagc gaagtgttcc atctctgccg ggtgatgtat ctcgataaag agccgctgtt
    33301 tattgatagt tcatggatcc cgctgtcgcg ttatcctgac tttgatgaga tttacgtcga
    33361 aggaagctcc acctatcagt tatttcagga gcgttttgac acgcgagtgg tcagcgacaa
    33421 aaagaccatc gatatctttg ccgccacccg cccgcaggca aaatggctga aatgcgaact
    33481 gggcgaaccg ttgtttcgca tcagcaaaat cgcctttgat cagaatgaca aaccggtgca
    33541 cgtctccgaa ctcttctgcc gcgccaatcg catcacctta acgattgata ataaaagaca
    33601 ttaaccgtag gccggataag atgcgccagc atcgcatccg gcgatgctgg cgcactgaat
    33661 tttacatccc gcacgttccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtt
    33721 cagtgcggaa tttagcttga ttgccgaacg gtgctatcca gcattaaata ctcgcaatac
    33781 tctcacgcaa aatccgcagc accgtctctt caccgctgcg attcgggtta atgcgaatcg
    33841 cacaatgttc gagttgtgga ttcgcctggc gaaacgttcc tgaaagtcga tagaagagcg
    33901 gcgggatttc atatttcgac tctgcaccga ccgggtaagg caacgcgcca cgcttttgcg
    33961 cttcttccag caccctggcg gcaatcagct gatgaaactc gacaatcaac accttcgact
    34021 gcgcattggc aatgacggcg cttttcactt ccgccaccgc gccgccgtta agcaaagcca
    34081 gcaaccgttc ggatactccc gcctgcaccg catgcatcac tggcgcgaac accagaccac
    34141 gcaagacttc cagcgcctgt gagccctgga tctggctacc gccggagtaa agcgtggcgc
    34201 gaatccgact aataacatcg gcatcgccaa ccactgcacc aacgccctcc ggcccaaaca
    34261 gcttgaagca ggagaatgtc gagacattcg cgccgcattc acagccgatt cgcgctacct
    34321 tcatcaccgc atagttatca tcggttaacg ctggaacacc tgccgcgcgc aacgttgcca
    34381 gcacatctgc cagcacatag ctgtcctgcg gctgctggcg cgtatgctgc accagcgcgg
    34441 catcaggttg ttgctcgtcg acgacctgct tcagtgccga caggtcattg aaatcagcag
    34501 taataagcgt cagccccatc tgctcaataa taacccgtgt cgtcgggtaa acaggcgcgt
    34561 catgcaccag aagacgctgc cccggtttga gcaaagccgc cagcccggcg cgaatcgcgc
    34621 cagtccccgc gccctgcacc agcgccgcgg cctgtgcgtg aaatgcatca gccagcacct
    34681 gctccacccg ttgggtaata cgcggttgat tcagacttgg agttaaccct aaatcaccgc
    34741 cagcaagaaa ctcgctgcct ggaaaatggc ggcaaatagt gtccaccagc gcaaactgct
    34801 tttgctgcgc ttcagcaaga gtcaggcttt gcaaaggaaa cgtcttcata ggtgccttcc
    34861 ttaagccgga acgaacaggc caagccagta aagaacattc agcagaatac cggtgatcat
    34921 tactgccacg accggtgccg ccattttctg taccggacgg cccagggatt cgttgaggaa
    34981 gtaaatggca accgcgatag agaatccggt ataacccgcc atcttaattg ctgcgaaaat
    35041 cgaaccgacc agcagcgcca cttccatcag catattcatg gcgttacgga tgttatccga
    35101 cgcattacgc accgacgggt agcgtcccag ccatttgccg atcgaacgaa gcagcaagac
    35161 ttccgccgaa ataaccactg cgcctaatac cgctgcaacc atcggattcg gcgagagata
    35221 gcccaccgca taaacaaagg taaagcccgc aactgcatac acacccgttg ctaaggcggt
    35281 ggtggcaatc aacggcacaa accccagtcc gcgcataaat tctgccagag ccgcctgatt
    35341 aatcagcgtt tgcgattgtt ccggcgtcac gcctgcggaa tacgctttct ccagtgtgaa
    35401 gatcgaaact tcgctaccag caaaaatctt catactggca acggcggcaa tcaatgctcc
    35461 cacgatggcg atataaggta agtttttgat aatccgcgac gtgcgctctt caaacaccga
    35521 aagcccgctg gcatcgatgt cattttcatc acgatgacgc aggtcgtgag ttatcgcgat
    35581 ccccagcagc atcaccatgc caataaagat ttcgatggat tcagggttaa gatgtgggaa
    35641 atagcgcacg actaccacac gggtcatcag aacaaccacg gcggcgacca gactttgctt
    35701 ccagccaaac tggtagaaaa tcgccaccag cgggaacagt gcaaaagcag aaaccaccgg
    35761 cgagcttaat tccccgaggc tacctaatac atccaccggc agcgcggtca gcagctggtt
    35821 aactggcaac aggcaagtaa ggatcaacac gccccagata gcgccaagac caaacgccat
    35881 caggctgttt atcgccagta cgccgaggat atcggtagga agaaagagta accatgcgtt
    35941 gagcagcccg gttttcaggg taaaagagat ccccaccgag gccacaaaac caatgctcag
    36001 accaaaagcg atactccccg cctcgcgacg gttcatatag ccttcaatca gttgcggcag
    36061 gatcgggtgg atgccgtcat gaaaaacagc cgccgagcga tgctccagaa gcgatgtcat
    36121 acccgtcagg cacgccacca cgataatctg aatatacaga tccatagtcg tgccttttat
    36181 ttcagatggt taatcaacat cggaatcgcg tgttcgacgt gctcaacgga aaggccaaac
    36241 gccactttcc cttcagcgat cattttggcg atatgctcgt ctttcgcttt aatgcctggt
    36301 ttggcaatgg tgcaactttt gttatagcca atcaccgcga tggcaatcga caatgccgcg
    36361 cccgcaccgg tattacacgc gccaatgtaa taatctagtt gcccggattt caccttcatt
    36421 gccgcttcca tgtcgttgtg aatgaaaact tcaaaacagc caggagccgt cgcttcaatg
    36481 gtttttttaa tctgctcacg ctgtaagcct gcaacgccaa tctttttcat gagtctgtcc
    36541 ttattggaaa aattgagagg gattttcacg taacatcaca tccacatcgg cctgactgaa
    36601 tcctgactgg cgcaattgcg gaataaaggt ggtcaataaa tagtcatagc cataaccacc
    36661 attggctttt aaatgggagc ggcgcgtaat atccatcgac agcatgacgc ggttcagcaa
    36721 cccacggtca cgtagcgcgt ggagcatcgc aatgcgcttt tcgtccgggt agtaactgtt
    36781 cttaccaatg gtgtcgaact gcacgtacgc gccgagatcg atcatcttca aaatgttgtc
    36841 gaggttgtct ttcagatcgc agtgaccaac ggtgacgcgc gaaagatcaa ctccgtgggc
    36901 ttgcaacaga gccagttgtt ccagccccat ggtgctgaac gacgtatgcg tggagatcgg
    36961 gcgtccggtc tggttatgcg ccagcgcagc ggcaataaat accttctctt ccagcggcgt
    37021 aatctttcct tcgctggagc cgatctccgc gatgatcccg gctttcagct ccgtgccatc
    37081 gataccctgt tcaatttcat cgaccatctc ctgcgccagt tcctgcacgc tgcgggtcac
    37141 cacatgttcc gggaaaaacg catcctggta ataaccagta caggccacca cgttgatccc
    37201 ggtttcgcgc attacatcga gcataaattg cgcattgcgc cccatgtaac ggttggtcat
    37261 ctcaatcaca ttacgtacac cccgggtcat caggtcgttc atctcctggc aaatgaacgc
    37321 atactgatca aggcggcagt ccacgttgtt tttaaagccg gagagatcaa tatgcagatg
    37381 ctcatgggcc agggtgtaac ccgtcggatc aaaactcata gggtgtctcc tgcaaaccgg
    37441 agggaggaaa gaaaagagcg accattttgt ggcggtggcg cacggaaaaa ttcacacacg
    37501 gtagctccca catcagaaag cgtggtgcgc acaccgagct gcgtggcgac cagcccttgc
    37561 tgataaacca gcactggcac cacttcacgg gtatggtggc tgtgaccaat ggtcggatcg
    37621 ttaccgtgat ccgccatcac gaccaggcaa tcatctggct gcatcgcctc aacaagccgg
    37681 gcaaggttac ggtcaacgac ctgcaaacgt tcggcataac gtgcgacgtc ttctgcatga
    37741 ccagcgaggt cggtttcctg gatattgatg caaataaacg ccgtcggatg ggtgttaaat
    37801 tcgtcgaggg tgatatccat aatccgctgg ctatccacca ggttttgcca gctcacacca
    37861 taaggattgc tgacgatatc tgccacctta cccaccagca cggttggcac gcccgcttca
    37921 tacagttttt gcggcacctg cactttttca tcaacaccgt agcccatatg cacgacctgg
    37981 aagccgttgt cataagcgcc agaacgcggc gcattaatac caataaagcg cccttctttg
    38041 ctttctgcgg catcgagaat acgctgactg ttggttaaca ggccgccaaa tgtaatgacc
    38101 cgaccgacct gtacctggtc acgcacgata cgaccaattt tgattgcgtc gtcaaaagag
    38161 atcacagaga gattggcggt aatgttatag acttggccta aatccgcctc gagattatcg
    38221 ccaatcgcaa ccgcctgatt gaccgacaga aattgcagat catcgccacg gcgctccacc
    38281 tgccagccag cggaaactaa tgcctgctca acacggtcaa tcacatcgcg aaaaggcatc
    38341 cgcagcggcg gtaacgggcg cgtgcctaaa atttcctgat gccccataaa ggtatcgcca
    38401 ccttcatgtt gcagctctgc cacgccccag gttgcggaat ctgacggctg catatcgcct
    38461 ggcgcataac ccaatgtgtt gattagcccc agcttctcca gcgttggtag ctgcaaatgc
    38521 ggcaactggc tcaggatgtg accacaggta ttcgccccgg catcttgcgg gcgcaccagc
    38581 gtgacatctt tcattgcccc tacgccaaag ctatcaatca ctaacaccac aaatcgcgcc
    38641 atttatgcct ccagagggtt gccgaggctg tcataacgac caactatttc tggttcgccg
    38701 cgctgaatac cggacaccag caccacatcg ctacgggtga caaaaatctg cgtgcgaaaa
    38761 cagagcacca ctgcactgct caccggatac tcgcctgcca gcggcagggt gtagtcgata
    38821 ctgctgtcat ccacagtttt gagattggtt tcagtaatct tttgattttc tggcgtaaac
    38881 accagcgcat gttgcgcatg accacgacgg tagtaaccgc cgccgtagca gtagctgtcg
    38941 ccacggaaat gatgggagat ttcacttaac cagagcatcg cgatacgttc aggctgatcg
    39001 ccctgctggt ttgccggaat agtaccggtc agcgcatgac cgggttcggc atgagtcgca
    39061 ccgtattccg ccagcaatgg cagcgaagtg cagctggtcg ctgaaggtac gttcagttgc
    39121 tcaatcgcaa taccagattt cgccagttga tcccgtgctt gtaccagcgt gtgaagattc
    39181 ggtgttggca aaactttccc agcagcctca tcccaaagca ggcaagggaa atgggttagt
    39241 ccggctaaat gcagccccgg cagtttctta atttcagcaa tcacctcgtg cagcgaatgc
    39301 tgtacaaaac cgctctcctg ccccggataa agaaaatcat cgtcgctata aactttgagc
    39361 agcacaggct ggacccgtcc ggccttaacc gctgccgcag aaacttcccg cgctttgtcg
    39421 agagtaaaca cggtgatgat gtcggtgccc tgttcaacgg cgtcagcaac ctgatgacaa
    39481 gggatttgta ccagatgccc ctgatgcgcc actggcaaac cagcgcggcg catgactcgc
    39541 gcctctttgt aatccaccgc cacaatgccg ctgtagccta atgccagcaa tttttccgcc
    39601 agccacggat tacgaccaaa ttgcttggtc atcagataca gttcaatgcc gtaaagccgc
    39661 gccgtctcaa tgagccgctt accgttttcc agtatctgat ccacgtcgat cacccagctg
    39721 tccggggcga ttttgccctg ctgccacaaa cttagtgcgg cggaaatcag cgccgggttc
    39781 tggcgtttca atgcttctac aaacatcttg ttgcctcgtt tctgttcctt gaattaaata
    39841 ttcatttttt tgaatattta ggttgtgcga cgtggatttg tcgggagctt caaacctctt
    39901 cgttagcagc catccataat ccataaagat tggccagaaa atagccttct tcacacgggt
    39961 taacttccag agcgaattcc ttcaacaact cctgatgcaa ttgcacaacg gcttgccagt
    40021 ggctggattg cgccagttcc gccagcaact cgttatccag cggctctatt tcttcaccac
    40081 ggcgactgcg catcagtgca ctcgccatat gtgtcatcgc catcgttcct tgctcactgc
    40141 gcaccggcag atggcactct ttttccagta cgttgacgac ctgcatcatg cctttgcaga
    40201 cgtccttatc aataacgcct gcctcgcaaa gcaggttgag tctggtttcc atatcagatg
    40261 cctttcgttt aataactggg ttcctgttcc ccactcacca cccgttgttg atgggcaagc
    40321 agggcgtgtt tatctacaac agcacgcagc aattgttgca ttgggtaatc atcgactcgc
    40381 gtcaggacca cggcttcggt ggcctgtaaa aatcgcggat cgtctgttag cggcgtcgcc
    40441 tctaatccca gcatggtcag ttcgttttcc gccaccacgt tccagatcac cgcatcgaca
    40501 tcgcctttaa caatgcgttg taaactctcg tgataagaga gatcgactcg ttccacatca
    40561 ctatcgccaa aaaagacatc ggtcatgatt ttctgatccg ccgaacgact atcgagacct
    40621 actcgcttaa catttccaga ctcacctttg cggcatatca gttgatgttc accaacatac
    40681 gtatgcgggc caagctcgag ggcgatgcac aaattgtttt ggctgagata actttccgcc
    40741 gccaggcggg aaactactgc catgtcatag acaccgttaa gtaaacactc cacgcgaata
    40801 tccgcgccgc gcatatgcgc atagtaaaaa ggaatgccat caaactgggc tttcaatccg
    40861 ctcgctaaac cttcgtacaa gcgggtatag ggcaaaggca tcgcacatac cacgttgttg
    40921 atatccacat gactcagcaa tgctttgtta tccatctcga caagataact gccattgcgc
    40981 ccacggcgtt caatccgtat cgctccgctt gattccagcg ttttcaacgc ggcctgcgtc
    41041 aggccaaccg atgaacggca ttcagttgcc agctcatcta tggttttcag tcgattaccg
    41101 catttttcac ccaacaaata acgggccagt gtcgtgatga cgacgccttc ttttttgata
    41161 aacgttcgac gcataatata ttttcaataa aatgaatatt tatatcttca ttaaaatgaa
    41221 gataagtggc gacgacgaga tgtggaaacg gcgaggcgct tcacattttt tcgggttgtt
    41281 gcgaaaagag gataaaaaaa ccgggtttcc ccggtttcag agtgatgacg aagtgattta
    41341 ttcgacaatg cgagcaaagg gtaattccca ctggcgcttg cctgctcccc tcaccctaac
    41401 cctctcccca aaggggcgag gggaccgtcc gagcgttttg atattacggc ttcgccacaa
    41461 aaccaatcgc ttcgtacacc gctttcagcg tacgggaagc gtgtacactg gctttttctg
    41521 cgccgtcttt catcacctgt tgcaggaagg cttcatcgtt gcggaaacgg tgatagcgtt
    41581 cctgcaattc agtcagcata ccggaaacgg catcagccac ttcacctttc agatgaccat
    41641 acatcttgcc ttcgaactgt ttttccagtt ctgggatgct ctggcccgtt accgcagaaa
    41701 ggatatccag caggttggaa acgcccgctt tgttctgcac atcgtagcga actaccggcg
    41761 gctcgtcgga gtcagtgacc gcgcgtttga ttttcttcac taccgatttc ggatcttcca
    41821 gcaggccgat aacgttattg cggttatcgt cagacttgga catcttcttg gtcggctcca
    41881 gcagcgacat tacgcgcgcg ccagatttcg gaataaacgg ctccggcacc ttaaagatct
    41941 cgccatacag cgcgttgaaa cgctgggcga tatcgcgact cagttccagg tgctgtttct
    42001 ggtcttcacc caccggtacc agattagttt gatacagcag gatgtccgca gccatcagca
    42061 ccggatagcc aaacagacca gcgttgatgt tctcggcata acgcgcagat ttatctttaa
    42121 actgcgtcat gcgactcagt tcgccgaagt aggtatagca gttcagtgcc cagcctaact
    42181 gcgcatgttc tggtacgtgg gactgaacaa aaatggtgct tttctcagga tcgataccac
    42241 aagccagata caaggccagc gtatccaacg tcgctttacg cagcttctgt gcatcctggc
    42301 gcacggtgat cgcgtgttgg tcaacgatac agtaaatgca atggtagtca tcctgcatgt
    42361 ttacccactg acgcagcgca cccatgtagt taccaatggt caattcacct gagggctgtg
    42421 cgccactaaa aacgatgggc ttagtcattt ttcgattcct gattttcgct atgcggaagc
    42481 cctaatgcgg gcagaaggtc atttatagac tgataaatta catcaggctg gctgagatcg
    42541 atagcctcgc cgtagttgta tccgtaggtt aagccaactg atgggcaacc tgccgctttt
    42601 gccgcctgaa tatcattccg tgagtcgccg acaaacagca tctgttgtgg ggcaattccc
    42661 atccgctcag ccaccagtaa cagcgggtcc ggatgcggtt ttttgttttg cacatcatcg
    42721 ccgccgataa ccacgctaaa gtatttggca atatctaagg cttcgagcag cggcgcgacg
    42781 aacggcgtcg gtttgttggt gaccaagcct agcggcaggc ctttagcctg caacgcgccc
    42841 aacgtatcgg caacgtgcgg gaacaaaaac gtcccctctt cggcaacctc gccatagtag
    42901 cgatcgaaca gtttacgcag aatacgtacc tgttcttctg ccggaatgtc gtcatcaacg
    42961 ggcggtttcc ccattgtttt acgcagagtc gcacgttcct gacgcgccca ggtcaatgcg
    43021 cgctccatca gaacatctgc gccgttacca atccaggtaa taacgcgttc ttcacctgcg
    43081 acgggcaact ccagcgcata cagcgccata tctaccgcag cagcaagacc aggagcactg
    43141 tcgaccagcg taccatcaag atcaaaagcg acgccgcgaa tatcttcaaa cttattcatg
    43201 acttaccttt gccagttcac tgcgcatttc atcaatgact tttttatagt ctggctggtc
    43261 gaagattgcc gaaccggcga cgaacatatc cgcgcccgcc gcagcgattt cgccaatgtt
    43321 gttcaccttc acgccaccgt ccacttccag acgaatgtca aagccagact cgtcgatacg
    43381 gcgacgtact tcgcgcagtt tatccagtgt ttgaggaatg aaagactgac cgccgaaacc
    43441 agggttgacg gacatcagca ggatcacatc cagcttatcc atcacgtaat ccagatagct
    43501 cagaggtgtc gccgggttaa ataccagacc cgctttacag ccattttctt taatcagttg
    43561 cagcgtgcgg tcaacatgct cggaggcttc tggatgaaag gtaatgatgc tggcaccagc
    43621 ggcagcgaaa tcaggcacaa tgcgatcgac gggtttcacc atcagatgta cgtcgatagg
    43681 ggcggtaatg ccgtagttac gcaaggattt cagcaccatt ggcccaatcg tcagattggg
    43741 aacatagtgg ttatccatga cgtcaaaatg cacgacatca gcgccagctg ccagggcttt
    43801 tgcggtatct tcacccaggc gggcaaaatc agccgacaga attgaggggg caatcaaata
    43861 ctgtttcatc cgcttctcct tgagaattat tttttcgcgg gtgaaacgac tcctggtttg
    43921 tacaaagcca gcagttcgtc caccttttta cgtgtgccgc cgttgctgct tatactgcgt
    43981 cgaactttga cgacatgcaa ttttgcgcgc tgataccact cacgcgttaa catcgtatcg
    44041 tgattggaga tcagcactgg aatatggcgc tcaaccagac cttcggcgat ctccgccaga
    44101 tgcgcttgtt gttcaagcgt aaaactgttt gtgtgatacg ccgtaaagtt ggcggtcgca
    44161 gacagcggtg cataaggcgg atcgcaatag acgacggatg catcatctgc gcgctccatg
    44221 ctatcggcgt aagactcaca atagaaaaag gcattctgcg ctttttcagc gaagtgatac
    44281 aactctgctt ccgggaaata gggttttttg tagcggccaa acggcacgtt aaactcaccg
    44341 cgcagattgt aacgacacag gccgttgtaa ccgtagcggt tcaaatataa aaacagtacc
    44401 gcccgacgga acggatcctg gcttttgttg aactcttcgc ggaactgata gtaaacctcg
    44461 gcgcaatttg tttcgggaac aaacagctcg cgtgcggcct gtacgtactc atcagtacgc
    44521 atcttcacaa tgttatagag actgatcagg tcgctattga tatcggcaag gatataacga
    44581 gaaaagtcgg tgttgagaaa caccgacccg gcacctacaa aaggctcaac cagacattcg
    44641 cccttgggca aatgccgttt aatatcatca agcaggggat acttgccccc tgcccacttc
    44701 aaaaaagcgc gatttttctt catgccgatt aactaattac accttctccg gctgtggaga
    44761 aagctccgac agcatccagc gcttcaggca gctcccgcaa cgataaacca ttgcgggaga
    44821 taaccttgat tacttcagat cggcctgtac ctgacgcagc ggtttcgccc acgggttttt
    44881 ggcctgaaca tctgctggca atgtagatac cgcttttttc gcctcttctt tcgaagcgta
    44941 cacgccagaa accaggacat accacggctg accattacgc gtcgtttcat agacaacgta
    45001 gtttttcaga ttctctttct tcgcccaacc gttcaggttg tcgtagttag aggaactgct
    45061 cagctgcaga gtgtaatggc tggacggtgc cgatttcaac gaaccaacat tacctgcggt
    45121 cttccctcca gcggctggtg ttgccgtggt ttgcgccggg gatgccgtct gtactggagc
    45181 cgtagtcgcc gtctcttttg gtgctggcgt agaagtcgca gccggtgcct tcgtgctcgc
    45241 cacaggggca gctggttcag gttcagtacg cttcggcgtc tgtgctaccg gcttcggctc
    45301 cgttttcacg gttgcttgcg gttttttcgg ttcaatcacc gcctgctgac gaactgggcg
    45361 cgtagtggac ggacgttcag cggtttgcgt tttcgccgtg tcacgcgatg cattgccatt
    45421 gcgaacaggc gcaaccgttg cgggttcagt cggcaacgtg gaattgaccg ccacattgtt
    45481 cagctgttgc tgattttgtg gctgggtcag cgcattgttc aggtcaccct gcacttcaac
    45541 acgttgttga ccatccgttg ccaccggggt ttgcccttga gtcggcgtag aagagatcgg
    45601 tggcagagaa acatcctgct gagtattttc cgcagacgtg gttcccggcg ctggctgcac
    45661 gccattcgcc tgatcggtcg cattgccagc aagatcaata ctcttctcgc cagacgcggt
    45721 ttgatcgctg gaagtggtcg agggggcttt tagcgcagaa ccgataccga tgatcaacag
    45781 cagtagaacc agaatgccga cgcccatcat catatactga cgagaagcgg gtttactggc
    45841 tgcttttttg cgcttacgcg gacgacgctc tacgcgctct tcatccacgg tttcatcttc
    45901 ggattcgtca atttcttctt cgatttccgg ttcctcattg cgctcttttt gcgcacgagt
    45961 cggacggcga tcgtcagtgt catcaagttc aatatcatca aaattgatct gcggttcgcc
    46021 acgttcagta cgctcagaac gttcagaaga ttgacgagaa cgaccagtac gacgatcgct
    46081 gggatcgggt ttcagctcgt cttctggttt gaattcatcc atttaacacc ccactaaaag
    46141 gttaatgctt accacgttgc aattaacctg aagctaatag accgcttgat aagcggcctg
    46201 acctttcttg ttgttacgct gattgacaat cggcaatggc gttaagaaca agctcgtgcg
    46261 aaacgccgct gcgaacttca ctcttaccaa ttgccaacgg aagaattaag cgcatctctc
    46321 ccgcaaggac tttcttgtca cgcagcatat gcggtaaata cgcctgcgcg gacatttcgc
    46381 gcggcccatt gaccggtaac ccagcccgcg tgagcagggt tataatacgc tgcgtttcgg
    46441 cagaactaaa ctgcccgaga cgttccgacg tccgcgccgc catcaccata cccgcagcga
    46501 ccgcttcacc atgtaaccaa ttgccatacc ccatttcagc ttcaatggca tgaccaaagg
    46561 tgtgtcccag attcagtaaa gcacgtaacc cggtttcgcg ctcgtcggcg gcgacaactt
    46621 ctgccttcag ttcacaacaa cggcgaatac agtacgccat tgccggaccg tccagacgca
    46681 acaacgcatc cagattctct tccagccagt taaaaaacgc accgtcaaga ataatgccgt
    46741 atttgatgac ttctgccagc cccgacgcta actcacgcgg gggaagcgtt ttcagacagt
    46801 cgagatccac caccactgaa gcaggctggt agaacgcgcc aatcatgttt ttaccgaggg
    46861 gatggttgac cgcagttttg ccgccaacgg aggaatcgac ctgcgacagt aacgtcgtcg
    46921 ggacttgaat gaaacgaaca ccgcgctgat aactcgccgc cgcgaagccg gtcagatcgc
    46981 ccactacgcc gccgccaagc gccaccagcg tagtatcgcg accatgcggc ttttgtaaca
    47041 acgccgtaaa gacggtatcg agtacagcta ggcttttata ctgctcgccg tcagggagga
    47101 taacgctatc gacgttaaca cccgcctgtt caagtacgcc gcggacctta tcgagataca
    47161 gaggagccag ggtttcgctg gtgaccaaca tgacctgctc gcccgatttc agcggtaaga
    47221 atgaagctgg ttcattaaac aaaccagatg cgatggtaat tgggtaacta cgttccccga
    47281 gagtaacgac aatcctctcc ataacgcgac atccacctta attactgtac ccgcagacga
    47341 gtgtatataa agccagaatt agttgctttc cagcatgtga ataatctggt ttgcaaccac
    47401 tttagcgctt tgatcatcag tacgaatggt cacgtcggca atctcttcat acagcggatt
    47461 gcgttcattg gccaacgctt ccagaacttc acgcggcggt gtttcaacgt gcagcaacgg
    47521 gcgtttttta tcacgctgcg tgcgtgcaag ttgcttttcg atggtcgttt caagataaac
    47581 gacaacgcca cgagcggaaa gacggttacg cgtttcacgg gatttcacag agccgccgcc
    47641 agtagccagc acaataccct gtttctcggt caactcattg atgacctttt cttcgcgatc
    47701 gcggaagcct tcttcgcctt ctaaatcgaa aacccagccc acatcagctc cggttcgttt
    47761 ctcaatctct tgatcggaat cgtaaaattc catattgagt tgttgagcta actggcgccc
    47821 aatagtgctt tttccggcac ccataggccc aaccagaaag atattgcgtt tctctgccat
    47881 tttttcggta ctactaagac tattcgttaa tgataaaccc gcttcgctca gagagcgccg
    47941 caggacatga actgaaacct cataagatat tgcgagagtc agactgaaaa ttatctcaat
    48001 actccagcgg gtttggcaac tgaataaatc acccatccct ctgcatatct ggtcgctgca
    48061 agcgcgctgc cttgctacca ccgctctggc gataaatcac cgggtaagat tagcgtaaaa
    48121 aagacagcaa aatgccgcct gaatgataaa tcatcatcat ggaacgcagg cagcgaagag
    48181 caccaactct caaatcggta ctccttgtat gctaaatacc tgcgcgcgtc aaatagatga
    48241 aacacgttca gcataaaaac attaccgctt ttacggctgt ttactcactg gaaaccagtc
    48301 gtggcgtgat aaacaccact aactcgcgtc gttcatcttc ttttccgtca tgacgaaata
    48361 attgcccgaa ccagggaatg tcgccaagca acggtacgct atcctgaccc gatttatttt
    48421 tacgggtaaa aatgccgccc agcgccaacg tttctccgct ttttacctcg acctgcgttt
    48481 cgatctcctg cttatcaatc gccagcactt cgccatcggc ctgctgtagc acctgccccg
    48541 gaacgttctg gctgatgtgt aatttcagcc ggatgcgacc tttttgtaac accgtgggcg
    48601 tgacctccat ccccaggacg gcctctttaa attccaccga cgtcgcgcca ctttccccgc
    48661 tggaaacctg atatggaatt tcgctctcct gtttaatgct ggcaggctga agatgtgagg
    48721 ccagcagacg cggactggcg ataatatcca gctgctgttt ttgttcgagc gcggaaagct
    48781 caagatccag caagcgtccg ttgatgcgcc caatgttaaa accgacatgc gttgtcgccg
    48841 tcgctacgga gaggtcgcta ccaagcgtgg tgacttgccc aacgccacca gcgtgttgcg
    48901 catcggccag cgtccatttc acgcctaact cacgcaaact tttttcatta atggtgacaa
    48961 tatgcgccga cagctcaacc tgcccgaccg gcagatccat ttgcgctacc cactgttcaa
    49021 gcgcgcttaa cgccgttttg ttatctcgta gcaaaaggcg attggtgcgt ttatcgacgg
    49081 tcatactccc tttggcactc agtagcttct cccccgcttt cgccagttct cccgcgtcgg
    49141 cgtattgcag ggttatactg cgattttcca gcggcagatt tgcctgcgcc cgcgcctgct
    49201 ccgcctcctg gcgggcgata ttgtcattct gccaggcaat ggaatgcact gagagaatgt
    49261 tgccttcctg ccgcgttatc agtccggcgc ttttcactac agtttgtagt gcctgcttcc
    49321 agggaacatc tgttagatgt aacgacaccg taccgctgac gtctggcgac acgaccaggt
    49381 tcaacttctc ctgttcagcc agcgcctgca acacctgagc taccggaacg tcatccacca
    49441 tcagcgtcac tttttgcggc tttgccgcct gtacgccggg tatcagcatc aacagtagtg
    49501 cggctatcca ttgcttcatt tgtatctcct tgccgttgcc acaaccattg tggcggttcg
    49561 cagtttgtcc cggtacccag cgttagtacg tctggcgtca gctgtaaaat tgtccagccg
    49621 ttttccagca catcgttttg ctgcacccgt cgccatttct tttgcccgtc ttttattaca
    49681 ccgatgatgc gctcgcctcg ccctaccatc ccctgatagc gccactggct aagttcgcta
    49741 atccggcata tagatcttcc ggcgatttaa aagggtcacg cataccggtt aaaaggcaca
    49801 atgcaatacc tgccaacaac cagcgtttaa ccctcatgcg gcatctccag ttgtagcgtg
    49861 aacaaaagat catcaccttc cacgcttaac gaaaaacggc tcacgctgac gttgcgctct
    49921 gccagccgtg taaatgccga cggcactgct tcccacagcg ttttcaacgc caactcacct
    49981 ccctgcgcgg atggatgcca ggaaaccagt tgcgcgccgg ataactgaaa atccagtggc
    50041 gaaaagggca gcgttttttc ctcgctgaag ggagtggtgt ctaccaggcg atacagtgcg
    50101 gcccactgct ggtgatgact tgcccgtagt cgaattaatg cgtcgcgctc ttcatggtgt
    50161 gtcgatgata gaaaaatgag cgtaactaac atcagcaacc agaatgccca gcaaaactgg
    50221 cggaggcggg gtgatgtggc gaaccaccag tcaaagaaca tgttcatcgc taaccttcct
    50281 tgttaactga tactcaaatt gccagcgtcc ctgcgcatcc tgctgcgtgg ctccccgctg
    50341 attgagatga aaagacgcat cctggcggag tgacgtttct agtgcgttta acgcggtaat
    50401 gcttgttgtc agccccttga tctccagcgt tccctgctgc cagcttatcg ttgtcagcca
    50461 ggcgtgctcg ggtaaaagcg ccgccagtgc ttccagcgca gattgccagt cgcgggtaaa
    50521 ttgtcgctgg cgctgccgtt gcgaacgctg ttcgcgtaac tgctgttgct ccagcaaacg
    50581 tggcttcgtt atctgtaagc tgcgggcgag ttgttgttcc gcctgaagca aaactgcgtc
    50641 tatgcgcgct tcggcgctgc ctgtcagacg cagtattagc gttatcccga cggccagcag
    50701 cagaggcgca acgaacatca gcaaccagaa acgcagaaaa gcggtccggc gttgctgtcg
    50761 ccagggcaaa aaattaattg gcggattcat cagtacgcct tcccaagcgc cagccccagc
    50821 gcgatggcaa agtctccacc aggcggcggt agcggcggct gacgaacaga aacggcctcc
    50881 caggggtcaa atccgccttc gccacatatc gcgacgctgt cgggatcaac ggataacgct
    50941 gccgccagct ccttcgcgct agtcatcccc accgccagtt tgcgccccca gctatagcgc
    51001 gtcgcccaca gccactgttc gttatcacgc caggccagac attgctgatg agaaggtaaa
    51061 aaaggcagga atcgctgtaa tgcactggca tccggggtga tcgcactaac atgaacacgc
    51121 aacctttctg ccagcgtaag cagcgttgcc agctctttgc tttgcgcggc agtcacgtta
    51181 taagcggggc tgagtgagtc ttcgctgtaa tcgaagcgca gggagtccgg atccatatcc
    51241 agctcgcggg ccatcgtccc tgacagccag gccgtttgct cccgctcacc aagggacatc
    51301 gacgggcgcg gaaatgaccg ctgtaatgtg cgactggcgg gaaacgccaa cataatgtga
    51361 tgacgctgcg gcagttcgcg actccacggt aacaacgttt tagccagctg ctgcgcatca
    51421 acaatccgcc catctttgat aatgtcgtgc gccagcggca accgccacca gcgttgcaaa
    51481 aagcattctt ttgcgccccg cacgatcgca accgctgccg cttcttgctg ttgtaaatgc
    51541 aaaccaattt gccagatctt aaatgtcatt gtgatgatct ccttatcacc cgtcactctg
    51601 acgggtatat caatgcgtct ggcttgcctt tatactaccg cgcgtttgtt tataaactgc
    51661 ccaaatgaaa ctaaatggga aatttccagt gaagttcgta aagtattttt tgatccttgc
    51721 agtctgttgc attctgctgg gagcaggctc gatttatggc ctataccgct acatcgagcc
    51781 acaactgccg gatgtggtga cattaaaaga tgttcgcctg caaattccga tgcagattta
    51841 cagcgccgat ggcgagctga ttgctcaata cggtgagaaa cgtcgtattc cggttacgtt
    51901 ggatcaaatc ccaccggaga tggtgaaagc ctttatcgcg acggaagaca gccgcttcta
    51961 cgagcatcac ggcgttgacc cggtggggat cttccgtgcg gcaagtgtgg cgctgttctc
    52021 cggccacgcg tcacaagggg cgagtaccat tacccagcag ctggcgagaa atttcttcct
    52081 cagtccagaa cgcacgatga tgcgtaagat taaggaagtc ttcctcgcga ttcgcattga
    52141 acagttgctg acgaaagacg aaatcctcga gctttatctg aacaagattt accttggtta
    52201 ccgcgcctat ggtgtcggtg ctgcggcaca agtctatttc ggtaaaacgg tcgaccaact
    52261 gacgctgaac gaaatggcgg tgatagccgg gttgccgaaa gcgccttcca cctttaatcc
    52321 gctctactca atggatcgtg ccgtcgcgcg gcgtaacgtc gtactgtcgc ggatgctgga
    52381 tgaagggtat atcacccagc aacagttcga tcagacacgc actgaggcga ttaacgctaa
    52441 ttatcacgcg ccggagattg ctttctctgc gccgtacctg agcgaaatgg tgcgccagga
    52501 gatgtataac cgttatggcg aaagtgccta tgaagacggt tatcgcattt acaccaccat
    52561 cacccgcaaa gtgcagcagg ccgcgcagca ggcggtacgt aataacgtgc tggactacga
    52621 catgcgccac ggctatcgcg gcccggcaaa tgtgctgtgg aaagtgggcg agtcggcgtg
    52681 ggataacaac aagattaccg atacgctgaa ggcgctgccg acctatggtc cgctgctgcc
    52741 tgccgcagtc accagcgcca atcctcaggg agcgacggcg atgctggcgg acgggtcgac
    52801 cgtcgcattg agtatggaag gcgttcgctg ggcgcgtcct taccgttcgg atactcagca
    52861 gggaccgacg ccgcgtaaag tgaccgatgt tctgcaaacg ggtcagcaaa tctgggttcg
    52921 tcaggttggc gatgcatggt ggctggcaca agtgccggaa gtgaactcgg cgctggtgtc
    52981 gatcaatccg caaaatggtg ccgttatggc gctggtcggt ggctttgatt tcaatcagag
    53041 caagtttaac cgcgccaccc aggcactgcg tcaggtgggt tccaacatca aaccgttcct
    53101 ctacaccgcg gcgatggata aaggtctgac gctggcaagt atgttgaacg atgtgccaat
    53161 ttctcgctgg gatgcaggtg ccggttctga ctggcagccg aagaactcac caccgcagta
    53221 tgctggtcca attcgcttac gtcaggggct gggtcagtcg aaaaacgtgg tgatggtacg
    53281 tgcaatgcgg gcgatgggcg tcgactacgc tgcagaatat ctgcaacgct tcggcttccc
    53341 ggcgcaaaac attgtccaca ccgaatcgct ggcgctgggt tcagcgtcct tcaccccaat
    53401 gcaggtggcg cgcggctacg cggtcatggc gaacggcggc ttcctagtgg acccgtggtt
    53461 tatcagcaaa attgaaaacg atcagggcgg cgtgattttc gaagcgaaac cgaaagtagc
    53521 ctgcccggaa tgcgatattc cagtgattta cggtgatacg cagaaatcga acgtgctgga
    53581 aaataacgat gttgaagatg tcgctatctc ccgcgagcag cagaatgttt ctgtaccaat
    53641 gccgcagctg gagcaggcaa atcaggcgtt agtggcgaag actggcgcgc aggagtacgc
    53701 accgcacgtc atcaacactc cgctggcatt cctgattaag agtgctttga acaccaatat
    53761 ctttggtgaa ccaggctggc agggtaccgg ctggcgtgca ggtcgtgatt tgcagcgtcg
    53821 cgatatcggc gggaaaaccg gtaccactaa cagttcgaaa gatgcgtggt tctcgggtta
    53881 cggtccgggc gttgtgacct cggtctggat tggctttgat gatcaccgtc gtaatctcgg
    53941 tcatacaacg gcttccggag cgattaaaga tcagatctca ggttacgaag gcggtgccaa
    54001 gagtgcccag cctgcatggg acgcttatat gaaagccgtt cttgaaggtg tgccggagca
    54061 gccgctgacg ccgcctccgg gtattgtgac ggtgaatatc gatcgcagca ccgggcagtt
    54121 agctaatggt ggcaacagcc gcgaagagta tttcatcgaa ggtacgcagc cgacacaaca
    54181 ggcggtgcac gaggtgggaa cgaccattat cgataatggt gaggcacagg aattgttctg
    54241 attaaaaagg cgcttcggcg ccttttcagt tggctgacaa agtgcatttg tttatgccgg
    54301 atgcgggtga acgcgttatc cggctaacaa aatcgtgaaa aatcaatata ttgcaggaac
    54361 cccataagcc tgataagcgt tgtgcatcag gcaaacttca cgcatttaca ctcgcccctg
    54421 ccctttcaac cattcgcgca cgaggaacag cgcgctgaca ttacgcgctt cgttgaagtc
    54481 agggtcttcc agcaaatcca tcatatgcgc cagcggccag cgcacctgtg gtagcggctc
    54541 tggctcatcg ccttccagcg attccgggta gagatcttgc gcgaccacga tattcatttt
    54601 gctggaaaag taagacggtg ccatgctgag cttcttcaaa aaagtcagat cgttcgcacc
    54661 aaatccaacc tcttctttta gctcgcggtt agcggcttcg tagacgcttt cacccggatc
    54721 aattaatcct ttcgaaaaac ctaattcgta ggattcagtt cccactgcgt attcgcggat
    54781 caggatcagg tgatcgtcca caatcggcac aatcatcact gcttcccggt tggttggacg
    54841 cattcgttca taaacacgcc gcacgccatt gctgaactcc agatccacgc tctcgacggt
    54901 aaacagtcgg gaacgggcta cagtttcaac attcagaatg gtgggttttt gtaatgattt
    54961 gttcatcgtg ggatctatgc tgtgaaatca gcggttattg tgcgatatcg gacacgcttt
    55021 cggcaatgtg aattgcatgt tatttacatt tatgtaactt aataaataat cgtcctcaaa
    55081 tcaaattaaa agtcaatagg ttgaaataac tccaggaatt tgctgatatt ccgccttcag
    55141 agggtttgct atgatcagcg gttactgtga tgtgcttaat gatgctcaag ttaagctcca
    55201 cgcttgccga tagccaaccg cagaatcatg tattgtgtcc ggtgcgactg accacgcctg
    55261 acagactaag taagatgggg aaagcatgag caccattgtg atttttttag ctgctttgct
    55321 ggcctgctca ctacttgcgg gatggctgat aaaagtgcga tccagacggc gtcagctgcc
    55381 ctggaccaac gccttcgcgg atgcgcaaac gcgtaaactc acacctgaag aacgtagcgc
    55441 cgttgaaaat tatcttgaga gcctgacgca ggtattacag gtgcctggcc caacggaagc
    55501 tagcgcggca ccgatctctc tggcgctgaa tgccgaaagc aacaacgtca tgatgctgac
    55561 acacgctatc acacgttacg gcatctctac cgacgatccg aataaatggc gttactacct
    55621 cgattcggta gaagtccacc tgcccccctt ctgggaacag tacatcaacg atgagaatac
    55681 cgttgaactg attcataccg attcgctgcc gttggttatt tcactcaacg gtcatacgct
    55741 gcaggagtac atgcaggaaa cgcgcggcta tgccttgcaa cctgttccgt ccacgcaggc
    55801 gtcgattcgc ggggaagaga gtgagcaaat cgagctactc aatattcgca aagaaacgca
    55861 tgaagaatat gcgctgagtc gtccgcgcgg gctgcgtgaa gcgttgctga tcgtcgcctc
    55921 cttcctgatg ttctttttct gcctgattac cccggatgta tttgttccgt ggctggcagg
    55981 cggcgcatta ctgctgctgg gcgcaggtct gtgggggcta ttcgcgcccc cggcaaaatc
    56041 ctccctgcga gaaattcatt gtctgcgcgg tacaccccgt cgttggggat tgtttggcga
    56101 aaacgatcag gaacagatca acaatatttc actcggtatt atcgacctgg tctatcccgc
    56161 acactggcag ccatacattg ctcaggatct cggtcaacaa accgatattg atatctatct
    56221 cgaccgccac gtagtgcgtc aggggcgata tctttcgctg catgatgaag taaaaaactt
    56281 tccgttacag cactggctgc gcagtacgat tatcgctgcg ggctcgctgc tagtgctgtt
    56341 tatgctgtta ttctggatcc cgctggatat gccgctgaaa ttcactctct catggatgaa
    56401 aggcgcacag accattgaag ccaccagcgt aaaacaactg gctgacgctg gcgtgcgggt
    56461 aggcgataca ttgcgtatta gcggtacggg aatgtgtaat attcgcactt ccggaacctg
    56521 gagcgcgaaa accaattcac cttttttacc gtttgactgc tcgcagatca tctggaatga
    56581 cgcccgctca ttaccattac cagaatctga actggtcaac aaagcgacgg cattgactga
    56641 agcagttaat cgccagctgc accctaaacc ggaagatgaa tctcgcgtca gtgcctcatt
    56701 acgttcagcg attcaaaaat ccggcatggt attgcttgat gattttggcg acattgtact
    56761 gaagacagcg gatttatgtt ctgccaaaga tgactgtgtg cgactgaaaa atgcgctggt
    56821 caatctcggc aacagtaaag actgggacgc gctggtaaaa cgcgccaacg ccgggaagct
    56881 cgatggcgtg aatgtgttat tacgcccggt gagtgctgaa tcgctggata acctggtggc
    56941 aacctccacc gcgccgttta tcacgcatga aacggcgcga gcggcacaat cactaaacag
    57001 tccggccccc ggcggattcc tgattgtcag cgacgaaggc agcgattttg ttgatcagcc
    57061 ctggccttcg gcatcacttt acgactaccc gccgcaagaa cagtggaacg ctttccagaa
    57121 actggcacaa atgctgatgc atacgccgtt taacgccgaa ggtatcgtca caaaaatctt
    57181 cactgacgcc aatggtacgc agcatattgg ccttcatccg atcccggatc gttccggcct
    57241 gtggcgctat ctcagcacca cattgctgct actgacgatg ctgggtagcg ccatttacaa
    57301 tggcgtacag gcctggcgtc gttaccagcg tcatcgcact cgcatgatgg agattcaggc
    57361 ctattatgaa agctgcctga acccgcaact gatcacccct tcagaaagcc ttatcgaata
    57421 acacgtttgc gcggcaggtt atgctaccct gtcgcgcaaa ttgcttcact ctggagattt
    57481 ccctcatgca tatcaacatt gcctggcagg acgtagatac cgttctgctg gatatggacg
    57541 gcacgttgct cgacctcgcc ttcgataact atttctggca aaagctggtg cctgaaacat
    57601 ggggcgcgaa aaacggggtt acgccacagg aagcgatgga atatatgcgc cagcaatatc
    57661 acgacgtaca gcatacgcta aactggtact gtcttgatta ctggagtgag caactgggtc
    57721 tggatatctg tgcgatgacc accgagatgg gaccgcgcgc cgtactgcgt gaagatacca
    57781 ttccgtttct tgaggcactg aaagccagcg gtaagcagcg aattttgctc accaatgctc
    57841 atccgcacaa cctggcggta aaacttgagc atacgggtct ggacgcacac cttgatttat
    57901 tactttccac ccacacattt ggttatccga aagaggatca gcggttatgg catgcggtgg
    57961 ccgaagctac gggtctgaaa gctgaaagaa cgctgtttat tgatgacagc gaagcgattc
    58021 tcgatgctgc cgcgcaattt ggtattcgtt actgcctcgg cgtgactaat cctgattccg
    58081 ggattgccga gaaacagtat caacgccatc cgtcactgaa tgactaccgc cgcctgatcc
    58141 cctcgctaat gtgaaggaga cgccatgaaa gagaaacctg ctgttgaggt tcgactggat
    58201 aaatggctat gggctgcccg tttttataaa acccgcgcgc tggcccgtga aatgattgaa
    58261 ggcggtaagg tgcattacaa cgggcagcgc agcaagccga gcaaaatcgt cgagctgaat
    58321 gccacgctca ctctgcgcca gggaaatgac gaacgcacgg tgattgtaaa ggcgattact
    58381 gaacagcgtc gccccgccag cgaggcagcc ttgctgtatg aagagactgc ggaaagtgta
    58441 gagaaacgcg aaaaaatggc gatggcacgt aaacttaatg ccttaaccat gccgcacccg
    58501 gaccgacgcc cggacaaaaa agagcgccgc gacctgttac gatttaaaca cggcgacagt
    58561 gaataactgt cacctgcaag agagatgatt atgccgcaac atgaccaatt acatcgctat
    58621 ctgtttgaaa attttgccgt tcgcggcgaa ctggtaaccg tttcggaaac cctgcaacag
    58681 atccttgaga accacgatta tccgcagccc gttaaaaacg tgctggcaga actgctggtt
    58741 gcgaccagcc tgttaaccgc tacgctgaag tttgatggtg atatcaccgt acagctgcag
    58801 ggcgacggtc cgatgaatct ggcggttatt aacggtaaca ataaccagca aatgcgcggt
    58861 gtggcgcgcg tgcagggcga aattccagaa aatgccgacc tgaaaacgct ggtcggcaat
    58921 ggttacgtgg tgatcaccat taccccgagc gaaggcgaac gctatcaggg cgtagttggt
    58981 ctggaaggtg ataccctggc ggcctgccta gaagattact ttatgcgttc tgaacagctg
    59041 ccgacgcgcc tgtttattcg caccggcgac gtagacggca aaccggctgc aggcggtatg
    59101 ttgttgcagg taatgcctgc gcaaaatacc caacaggacg actttgacca cctggcgacg
    59161 ctaaccgaaa ccatcaaaac cgaagaactg ctgaccttac cggcaaacga agtgttgtgg
    59221 cgtttgtatc acgaagaaga ggtgacggtt tacgatccgc aggatgtgga gttcaaatgc
    59281 acctgctcgc gtgaacgttg cgccgatgcg ctgaaaacgc tgcctgatga agaagttgat
    59341 agcatcctgg cggaagatgg cgaaattgac atgcattgtg attactgcgg taaccactat
    59401 ctgttcaatg cgatggatat tgctgaaatc cgcaacaacg cgtctccggc agatccgtaa
    59461 gttcattaat gagtatgtcc ggcagagagg gctctgccgg attttacgac ttaatggcct
    59521 gatgtgctgc gcttaccagc gcctatgaaa taaatcgcaa attattttgg tcagatacag
    59581 cgtcgtatcc gataaaagcg ttgtcactta cttaagttta tttccagcgt ttcaccatct
    59641 accgtcagat cgcgccacgc tttcggcctc gtccccagtt ttccatcctt cgcagctgtg
    59701 agtaattttt ctttcgttat ttcgttcgga agtaaactca tatcaagcgc gtcccattct
    59761 tttctgtctg agtcaaagcc atagacaatg actctgtcat cattaaaagc aaacaggatc
    59821 cgctccgcct gaccatcact attcagatct tgctcgacca gtacgcaggc gtctttttca
    59881 atacaggtca tcacgttata gcgaccctgg attaaggccg accagaatgt cgcatccggt
    59941 ttaacagaac caggggcaat taacacattt tcggcgaata ctttttccga tacctgttcc
    60001 tgaagatgtt gctctccatc taacgccatc agcagatccc gcgcgcgttt cgggtctttc
    60061 ataaacccgg catcgctttt cagcgactca agcgccgcac gtccatagcg accgctctgt
    60121 tcgagcatgt agatagttac ctggtctggc gtgtttttgc cgctctgata acgcgccata
    60181 tgactgttca cgctaatacg catactgtcc agcaccggcg aattaagaag caccagtatc
    60241 accaacacca ataacgaaac cgcaaggttc actttgccct gaagaacaag gggattttgc
    60301 ccattacgcc agacgatact gacaaaatac cccagcgacc acaccagtaa caccagctcc
    60361 gccagcgcgc cctgcaagcg gtcgacagtc cagccgtact gagcgacccg cagccataac
    60421 ccccaggtgg cgacgaacac atacagcgga gcaaccagca aagcggtttt aatcaggcaa
    60481 cgcaacggcc ccgtccaggg aagtgacgct ttttgcggat cgcggacaat agccattaag
    60541 atcaattgca aaaaggccag cgttaacagc aacccggcgg cggagatgtg gcgagaaatc
    60601 gcgctcaggc ccgtaaacgg cagggtgatg ataaacatca gggttagcaa tgatactaac
    60661 ggcagcaacc ccgtggcgat tagcgtgaac aacttttgaa tagagtcgat taaacgtgac
    60721 tgtgtacgcg cgaggatcac cgccagtgcg gtaaccagac caaatgtaag atacataaac
    60781 cagtcggttg caaaaaagag cgtattgaaa aacgtgatgc caacaagttt aaacaactca
    60841 ccccacagta aaagcaccag ccacgtcagg ccattggaga gaaaaatcac cagtaatata
    60901 agtacgttat gccatactga ctggtaaaag tagctatagc ggctactgcc attgcggata
    60961 cgaaggcttt gttgtatcca cggtagcaac aacattgcca tcaacagcag atagcagcca
    61021 aaatcccaaa tcgccttttc agctctccag gggttcatgc catcagtttg ccacttcagc
    61081 caaccgctca tccctgtcgt ggcaataaac accagcgcca gccatcccca aaggcgcttc
    61141 tgtttaaaag agatcacgga gaagagtaaa acggatgaaa acgcgacggt cgcaggcacg
    61201 ccataaacaa tccaactgtg attttttccg gcaagccagg cgataagcag gtagcaaacc
    61261 agcccctgaa gtaatccggt agcaatcatc ccctgaagta atccggtagc aatcatcccc
    61321 cagcgtgttg ccggtgaaag ttcaacgtta tccatgttgg ttatccagaa tcaaaaggtg
    61381 ggttaattat cgcatccggg cagtagtatt ttgctttttt cagaaaataa tcaaaaaaag
    61441 ttagcgtggt gaatcgatac tttaccggtt gaatttgcat caatttcatt caggaatgcg
    61501 attccactca caatattccc gccatataaa ccaagattta accttttgag aacattttcc
    61561 acacctaaaa tgctatttct gcgataatag caaccgtttc gtgacaggaa tcacggagtt
    61621 ttttgtcaaa tatgaatttc tccagatacg taaatctatg agccttgtcg cggttaacac
    61681 ccccaaaaag actttactat tcaggcaata catattggct aaggagcagt gaaatgcgcg
    61741 ttaacaatgg tttgcccccg caagaactcg aggcttatgg tatcagtgac gtacatgata
    61801 tcgtttacaa cccaagctac gacctgctgt atcaggaaga gctcgatccg agcctgacag
    61861 gttatgagcg cggggtgtta actaatctgg gtgccgttgc cgtcgatacc gggatcttca
    61921 ccggtcgttc accaaaagat aagtatatcg tccgtgacga taccactcgc gatactttct
    61981 ggtgggcaga caaaggcaaa ggtaagaacg acaacaaacc tctctctccg gaaacctggc
    62041 agcatctgaa aggcctggtg accaggcagc tttccggcaa acgtctgttc gttgtcgacg
    62101 ctttctgtgg tgcgaacccg gatactcgtc tttccgtccg tttcatcacc gaagtggcct
    62161 ggcaggcgca ttttgtcaaa aacatgttta ttcgcccgag cgatgaagag ctggcaggtt
    62221 tcaaaccaga ctttatcgtt atgaacggcg cgaagtgcac taacccgcag tggaaagaac
    62281 agggtctgaa ctccgaaaac ttcgtggcgt ttaacctgac cgagcgcatg cagctgattg
    62341 gcggcacctg gtacggcggc gaaatgaaga aagggatgtt ctcgatgatg aactacctgc
    62401 tgccgctgaa aggtatcgct tctatgcact gctccgccaa cgttggcgag aaaggcgatg
    62461 ttgcggtgtt cttcggcctt tccggcaccg gtaaaaccac cctttccacc gacccgaaac
    62521 gccgcctgat tggcgatgac gaacacggct gggacgatga cggcgtgttt aatttcgaag
    62581 gcggctgcta cgcaaaaact atcaagctgt cgaaagaagc ggaacctgaa atctacaacg
    62641 ctatccgtcg tgatgcgctg ctggaaaacg tcaccgtgcg tgaagatggc actatcgact
    62701 ttgatgatgg ttcaaaaacc gagaacaccc gcgtttctta tccaatctat cacatcgata
    62761 acatcgttaa gccggtttcc aaagcgggcc acgcgactaa ggttatcttc ctgactgctg
    62821 atgctttcgg cgtgttgccg ccggtttctc gcctgactgc cgatcaaacc cagtatcact
    62881 tcctctctgg cttcaccgcc aaactggccg gtactgagcg tggcatcacc gaaccgacgc
    62941 caaccttctc cgcttgcttc ggcgcggcgt tcctgtcgct gcacccgact cagtacgcag
    63001 aagtgctggt gaaacgtatg caggcggcgg gcgcgcaggc ttatctggtt aacactggct
    63061 ggaacggcac tggcaaacgt atctcgatta aagatacccg cgccattatc gacgccatcc
    63121 tcaacggttc gctggataat gcagaaacct tcactctgcc gatgtttaac ctggcgatcc
    63181 caaccgaact gccgggcgta gacacgaaga ttctcgatcc gcgtaacacc tacgcttctc
    63241 cggaacagtg gcaggaaaaa gccgaaaccc tggcgaaact gtttatcgac aacttcgata
    63301 aatacaccga cacccctgcg ggtgccgcgc tggtagcggc tggtccgaaa ctgtaatgat
    63361 ttgaagctgg agaatatcta tccagtatct tatagaaagc aaaacgggag gcaccttcgc
    63421 ctcccgttta tttacccttc ttttgtcgtg ccctgcgccc gcgttaccgg cactggcagc
    63481 caggcgcgaa tggaaagccc gccccgctcg ctggtgccaa gctccagcat cccgttatgg
    63541 ttatccacga tacgctgcac aattgccagc cctaatcccg tgccgctaat ggtgcgcgca
    63601 ctgtcgccgc ggacaaacgg ctggaacagg tgcttacgtt gttccggcgc aattcccgga
    63661 ccgtcatctt ccacctggaa ccaggcgcga ttcggctccg ttccgctgct gactttgatc
    63721 cagccattgc cataacgggc ggcgttgacc accatattcg ccaccgcgcg tttgatcgac
    63781 agcgggtgca ttttcacttc aatgctgccg gggtaaagcg cggtttcaat ttcccgctca
    63841 tagccacttt cggcagcaat cacctcaccg agcactgcat taagatccgc catttccatc
    63901 ggcatctcct gcccggtgcg caggtagtcg ataaactgct caatgatggc gttgcactct
    63961 tcgatatctt tattgatcga ttctgccaga tagccatcct gctcgctcat catctcagtc
    64021 gccaggcgaa tacgcgtcag cggcgtgcgc aagtcgtgac ttacccccgc catcagcagc
    64081 gtgcggtcat ccgccagttg cttaacacca gccgccatat ggttaaaggc acgggtaacg
    64141 gaacgcacct ccgaagcgcc atattcacgc agcggcggcg gaataatccc tttaccaacc
    64201 tgcaaggctg cgtgttcgag atcgaccaac ggtcggttct ggatacgaat aaacagccac
    64261 gccccgccta tcgccaatag cataatcgcc agcgtatagc ggaacagcgg agagaaatcg
    64321 ccctgatgaa tttcggtcag cggcacgcgt acccagatat tgggcgacag ccaggttttc
    64381 agccagacga caggcgaact tttgttgacc tcaacgcgca cttccgtcgg gccgcccagt
    64441 tgctgtgcca tctgatggct taagaattca tagtgttgcg cccaacgcag acctgcctct
    64501 tcggcagcct cgttggagta gagagagatc cccagctcac ggtagatctc ccgacggaaa
    64561 gcgggaggca caaccaactg cgtgccgtcc tccagttgca gtttgtcggt catcaacata
    64621 cgcacttcgt acgcgaggac tttattaaac tgctggaggc tcggcaaaat cgcgaagttc
    64681 agcaccacca gataagtcgt caccaggctg gcgaacagca aggtgacgat gagcaataac
    64741 gtacgggcaa atgaacttcg tggcgagaag cgcaatcgcc tcatgcttta gagccgtccg
    64801 gtacaaagac gtagcccagg ccccaaacgg tctgaatgta acgcggatgc gctggatctt
    64861 cttctaccat gcggcgcaga cgcgaaatct gcacgtcgat ggagcgttcc attgcggaat
    64921 attcacgacc acgggcaagg ttcatcagct tatcgcggga gagcggctca cgcggatggc
    64981 tgaccagtgc cttcagtacc gcaaactcac cgctggtgag cggcatcggc tcatcttcgc
    65041 ggaacatttc gcgcgtaccg aggttaagtt tgaacttacc gaaagcaatt accgcctctt
    65101 cctgtgacgg tgcgcctggc agttcgttcg cctgacgacg cagcaccgca cgaatacggg
    65161 ccagcagttc acgcgggtta aacggttttg gaatgtagtc gtcagcgcca atctccaggc
    65221 ctacgatacg gtccacttct tcccctttcg ccgtcaccat aatgatcggc atcgggttgc
    65281 tctgactacg aagacgtcgg caaatcgaca agccatcttc accaggtaac attaaatcca
    65341 gtaccataag atggaaagat tcacgagtca gcaggcgatc catctgttct gcattagcga
    65401 cgcttcgaac ctggaagcct tgttcggtga gataacgttc cagcagcgca cgcaggcgca
    65461 tgtcgtcatc gaccaccaga atcttgtagt tctcttgcat tgtttgtact cccaaaggtt
    65521 cgcaacaatt tgtaagcgtg tattcttaaa aaagctcacg ttcgtcacca gctaaatctg
    65581 gtatgaattt cagcctaaat tgttacaaag catattaaac agcagcttaa gtatacaatt
    65641 tattcggcga aacattattg attctgttga tatgatcacg ttatacccaa tgtgcgcatt
    65701 atcaaacaga caaagggaat caacgagatg aaaacgcccc tggttacccg ggaagggtat
    65761 gaaaaactca aacaagagct taattatctc tggcgtgaag aacgcccgga ggtcacaaaa
    65821 aaggtgacct gggccgcaag tctgggcgac cgcagcgaaa atgctgacta tcagtataat
    65881 aaaaagcgtc tgcgtgaaat cgaccgtcgc gtgcgctatc tcactaaatg cctggaaaat
    65941 ctcaaaatcg tcgattactc ccctcagcag gaaggcaaag tcttttttgg cgcgtgggtg
    66001 gagattgaaa acgacgatgg cgtgactcac cgtttccgta ttgtcggcta cgatgaaatt
    66061 tttggccgta aagattacat ctctatcgat tccccgatgg cccgcgcatt gctgaaaaaa
    66121 gaagtcggcg atctggcggt ggtgaatacc cctgccgggg aagcgaactg gtatgttaat
    66181 gctatcgagt acgtgaaacc gtaaggaaga gtcttaacct cctgccgatg gctggcattt
    66241 ttgccagcca gtccgtataa ctatcccctg atttttgatc cgaaaagatg aactcaaacc
    66301 atgatgaatg attcgttctg ccgcattatt gcgggtgaaa ttcaggcgcg cccggaacag
    66361 gttgacgctg ccgttcgcct gcttgacgaa gggaataccg tgccgtttat cgcacgttat
    66421 cgtaaggaaa tcaccggcgg tctggatgac acgcagctgc gtaatctgga aacgcgtctg
    66481 agctatctgc gcgagctgga agagagacgt caggcgatcc tcaaatccat ttccgagcaa
    66541 ggcaaactca ccgatgatct ggcgaaggcc atcaacgcca ccctaagcaa aaccgaactc
    66601 gaagacctct acctgcccta caaacctaaa cgccgcaccc gcgggcaaat cgccattgaa
    66661 gcagggcttg agccgttggc tgacctgctg tggagcgatc cgtcacacac gccagaagtc
    66721 gccgctgcac aatatgttga tgccgataaa ggcgtggcag ataccaaagc cgcgctggat
    66781 ggcgcgcgct atatcctgat ggaacggttt gccgaagatg ccacgctgct ggcgaaagtg
    66841 cgtgattatc tgtggaagaa cgcgcatttg gtttctacgg tggtgagcgg taaagaagag
    66901 gaaggggcga aattccgcga ctatttcgat catcacgaac cgttgtccac ggtgccttct
    66961 caccgcgcgc tggcgatgtt ccgtgggcgt aacgaaggcg tactccagct ttcgctgaat
    67021 gccgatccgc agttcgatga gccgcccaaa gagagctatt gcgagcaaat catcatggat
    67081 caccttggcc tgcgcctgaa caatgccccg gcggatagct ggcgcaaaag cgtagtgagc
    67141 tggacctggc gcatcaaggt gctgatgcat ctggaaaccg aactgatggg taccgtgcgc
    67201 gaacgcgcgg aagatgaagc aatcaacgtc tttgcccgta acctgcacga tctgctgatg
    67261 gcggcccctg ccggactgcg tgcaacgatg ggcctcgatc cgggtctgcg taccggggta
    67321 aaagtggcgg tggtcgatgc cactggcaaa ctggtggcga ccgataccat ttacccgcac
    67381 accggacaag ccgcaaaagc agcgatgacc gttgctgcct tgtgtgaaaa acataacgtt
    67441 gaactggtgg cgatcggcaa cggtacagct tcccgcgaaa ctgaacgttt ctatctcgac
    67501 gtgcagaagc agttcccgaa agtgaccgca cagaaagtga tcgtcagcga agctggcgcg
    67561 tcggtttact cagcttccga gctggcagcg caggagttcc cggatctcga cgtttcgctg
    67621 cgtggtgcgg tatctatcgc ccgccgtttg caggatccgc tggcggagct ggtgaaaatc
    67681 gatccgaaat ctatcggcgt aggtcagtat cagcatgacg tcagccagac gcaactggcc
    67741 cgcaaactgg atgcagtagt agaagactgc gtaaacgccg ttggcgtcga tctcaacacc
    67801 gcttctgttc cgctattaac ccgcgtggcg ggcctgacgc gcatgatggc gcaaaacatc
    67861 gtggcctggc gcgatgagaa cggccagttc cagaaccgtc agcaactgct gaaagtcagc
    67921 cgtctggggc cgaaagcctt cgagcagtgc gcgggcttcc tgcgcattaa ccacggtgat
    67981 aacccgctgg acgcctctac cgttcatccg gaagcctatc cggtggtgga acgcattctg
    68041 gcagcaacac agcaggcact gaaagatctg atgggtaaca gcagcgaact gcgtaacctg
    68101 aaagcgtctg actttactga tgaaaaattc ggtgtgccga cagtaactga catcatcaaa
    68161 gagctggaaa aaccgggtcg cgatccgcgt ccggaattta aaaccgctca gtttgccgat
    68221 ggcgtcgaga caatgaacga cctgcaaccg ggtatgatcc tcgaaggcgc agtgaccaac
    68281 gtcaccaact ttggcgcgtt tgtcgatatt ggcgtgcatc aggacggcct ggttcacatc
    68341 tcttcattgt cgaacaagtt tgtggaagat ccgcataccg tggtgaaagc gggcgacatt
    68401 gtgaaggtga aagtgctgga agtggatctt cagcgtaaac gtatcgccct gactatgcgt
    68461 ctggacgagc agcctggcga aaccaactct cgtcgcggcg gcggtaatga acgcccgcaa
    68521 aacaaccgcc cggcagccaa accacgcggt cgtgaagcgc agcctgccgg taatagcgcg
    68581 atgatggatg cgctggcggc agcgatgggt aaaaaacgtt aaacgcccgg actggcccct
    68641 aaacctgcgc gataaggcgt tcacgccgca tctggcaacg aacgctttgc ctgacataaa
    68701 agtgccggag aatatctccg gcacttttat tccacagcca aactcacaat atactccgtt
    68761 aatatttatc acttcattaa caactgaaac cttaattaaa cattagccag tccgggtaac
    68821 tcactattcg aattatattt tcgctgcgat ataaccttga gccacatcaa cattgagtca
    68881 gattattatt caaaccaaca ttcgcacaca ttttaagtat tgctgataga aaccattctc
    68941 attatcattg tgttgttgat tatttaatct ctcctttgtt ggcaaatcat ctggtctcat
    69001 gtcgcggtca aacgccccat gaggtagtta tccagttaat gagaaacaag taggcaccta
    69061 tgcaatacac tccagatact gcgtggaaaa tcactggctt ttcccgtgaa atcagcccgg
    69121 catatcgcca aaaactgctt tctctaggca tgttacctgg ctcctctttt gatgtggtgc
    69181 gcgtcgctcc actcggcgac cccattcata tcgaaacccg tcgtgtgagc ctggtattac
    69241 gcaaaaaaga tctggcctta ttagaagtgg aagcggtttc ctgttaatac ggtgataaca
    69301 acaatgaaaa aattaaccat tggcttaatt ggtaatccaa attctggcaa gacaacctta
    69361 tttaaccagc tcactggcgc acgtcagcgt gtaggtaact gggctggcgt taccgtcgaa
    69421 cgtaaagaag ggcaattctc caccaccgat catcaggtca cgctggtgga cctgcccggc
    69481 acctattctc tgaccaccat ctcatcgcag acctcgctcg atgagcaaat cgcctgtcac
    69541 tacattttga gtggcgacgc cgacctgctg attaacgtgg tggatgcgtc taaccttgag
    69601 cgtaacctgt acctgacgct gcaactgctg gaactcggca ttccctgcat cgtggcgctg
    69661 aacatgctcg acattgccga gaagcaaaat attcgtattg aaattgatgc tctatcggca
    69721 cgtcttggct gcccggtgat cccgctggtt tcaacccgtg gtcgcggtat tgaagcgctc
    69781 aagctggcga ttgatcgcta taaagctaac gagaatgtgg aactggtgca ttacgcacag
    69841 ccgctactca acgaagcaga ttcactggca aaagtgatgc cttccgacat cccgctgaaa
    69901 caacgtcgct ggctgggcct gcaaatgctg gaaggcgata tctacagccg cgcctacgcc
    69961 ggtgaagcgt cgcagcatct ggatgccacc ctcgcccgtc tgcgtaatga aatggatgat
    70021 ccggcgctgc acattgccga tgcgcgttac cagtgcattg ctgccatctg tgatgtggta
    70081 agcaacaccc tgacggcaga acccagccgt ttcaccactg cggtagataa aatcgtgctc
    70141 aaccgtttcc tcggtctgcc gattttcctc tttgtgatgt acctgatgtt cctgctggct
    70201 atcaacatcg gcggggcgtt acagccgctg tttaacgtcg gctccgtggc gctatttgtg
    70261 catggtattc aatggattgg ctacacgctc cacttcccgg actggctgac tatcttcctc
    70321 gcccagggcc tgggtggcgg tattaacacc gtgctgccac tggtgccgca gatcggcatg
    70381 atgtacctgt tcctctcctt ccttgaggac tccggctata tggcgcgtgc agcgtttgtg
    70441 atggaccgcc tgatgcaggc gctgggcttg ccggggaaat cctttgtgcc gctgatcgtc
    70501 ggtttcggtt gtaacgtacc gtcggtaatg ggcgcacgta cgcttgatgc accgcgtgaa
    70561 cgtctgatga ccatcatgat ggcaccgttt atgtcctgcg gcgcgcgtct ggctatcttc
    70621 gcagtattcg cggctgcctt cttcgggcag aacggtgccc tggcggtttt ctcgctgtat
    70681 atgctcggta tcgtgatggc ggtgctgact ggcctgatgc tcaagtacac catcatgcgc
    70741 ggtgaagcga cgccgtttgt catggagctg ccggtctacc atgtaccaca cgttaaaagc
    70801 ctgattatcc agacctggca gcgtctgaag ggcttcgttc tgcgtgctgg taaagtgatt
    70861 atcatcgtca gcattttcct gagcgctttc aacagcttct cgttgagcgg gaaaatcgtc
    70921 gataacatca acgactcggc gctggcttcc gtcagccggg tgataacccc tgtcttcaag
    70981 ccgattggcg tacatgaaga taactggcag gcaacggttg gcctgtttac gggcgcaatg
    71041 gcaaaagaag tggtggtggg tacgctcaac accctctaca ccgcagaaaa tattcaggac
    71101 gaagagttca atccggcgga attcaacctc ggtgaagagc tgttcagtgc ggtagatgaa
    71161 acctggcaga gcctgaaaga caccttcagc cttagcgtac tgatgaaccc cattgaagct
    71221 agcaaaggca acggtgaaat gggtaccggg gcgatgggcg tgatggatca gaaattcggt
    71281 agcgcagctg ccgcatacag ctacctgatt ttcgtcctgc tgtatgtacc atgtatctcg
    71341 gtgatgggag ccatcgcccg tgaatcaagc cgtggctgga tgggcttctc catcctgtgg
    71401 gggctgaata tcgcttactc actggcaaca ttgttctatc aggtcgccag ctacagtcag
    71461 cacccaactt acagcctggt gtgcattctg gcggtaatcc tgtttaacat cgtagctatc
    71521 ggtctgctgc gccgcgcgcg tagccgggtg gatatcgaac tgctggcaac ccgcaagtcg
    71581 gtaagcagtt gctgcgcagc cagcaccacc ggtgattgcc attaatggct tcacttattc
    71641 aggtacgtga tttgctggcg ttacggggcc gtatggaagc gacccagata agccagacat
    71701 tgaacactcc gcagccaatg attaacgcca tgctgcaaca gctggaaagc atgggcaaag
    71761 ccgtgcggat tcaggaagaa cctgacggct gcctctctgg cagttgtaaa agctgcccgg
    71821 aaggcaaagc ctgtctgcgc gagtggtggg cgctgcgtta accatactcc atcgcctgtt
    71881 ttgaaaagcc cggtatgcgt ttgcatccgg gcttttttgc gtgcggcttt ccataaaaat
    71941 gcaattcttg cagcgcaacg taagttcctc tgaaagcgtc tcgcagggat gaaaactcgc
    72001 taatgcacag atgtggagtg gcgcgtagag tcgcggcatt caaacaacag gtgaaggaac
    72061 gccatgagta aaaagcagag ttccacccca catgatgcgc tgttcaaact ctttttacgc
    72121 caaccggaga cggcacgcga ttttcttgcg tttcatttac cggcacccat tcacgcgctc
    72181 tgtgatatga aaaccctcaa gctggagtcg aggagcttta ttgatgacga tctgcgtgaa
    72241 agctattccg atgtgctgtg gtcggtgaaa acggaacaag gaccaggata catctattgt
    72301 ctgattgaac atcaaagcac ctcaaacaaa ctgatcgcat ttcgcatgat gcgttacgct
    72361 attgccgcaa tgcaaaatca ccttgatgct ggatacaaaa cgttgccgat ggtggtgcca
    72421 ttgttgtttt accacggtat tgaaagcccc tatccctatt cgctgtgttg gctggattgt
    72481 ttcgccgatc ccaaactggc aaggcagctt tatgcctccg catttccgct gattgatgtc
    72541 accgtcatgc ctgatgatga aatcatgcag caccgacgta tggcgctgct ggagttaatt
    72601 caaaaacata ttcgtcaacg cgatctgatg gggctggtag agcaaatggc ctgcttatta
    72661 agtagtggat acgctaatga cagacaaatc aaagggctgt ttaattacat actgcaaact
    72721 ggcgacgctg tacgttttaa cgattttatc gacggcgttg ccgaacgttc accgaaacac
    72781 aaggagagtt taatgactat tgcggaaaga ttgcggcagg agggggaaca atccaaagcc
    72841 ctgcatatag ccaaaataat gcttgaatcc ggagtccctc ttgcagacat catgcgattt
    72901 accgggctgt cagaagaaga gttggctgcg gcgagtagac tggccccctg aatctccaga
    72961 caaccag
//
LOCUS       DAJTQQ010000005        72057 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000005 DAJTQQ010000000
VERSION     DAJTQQ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72057
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.005"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(1335..2921)
                     /locus_tag="OO309_000388"
     CDS             complement(1335..2921)
                     /locus_tag="OO309_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HCR5845661.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
     gene            complement(3276..5447)
                     /gene="glcB"
                     /locus_tag="OO309_000389"
     CDS             complement(3276..5447)
                     /gene="glcB"
                     /locus_tag="OO309_000389"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCR5845662.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(5469..5873)
                     /locus_tag="OO309_000390"
     CDS             complement(5469..5873)
                     /locus_tag="OO309_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCR5845663.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5878..7097)
                     /gene="glcF"
                     /locus_tag="OO309_000391"
                     /pseudo
     CDS             complement(5878..7097)
                     /gene="glcF"
                     /locus_tag="OO309_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(7108..8160)
                     /gene="glcE"
                     /locus_tag="OO309_000392"
     CDS             complement(7108..8160)
                     /gene="glcE"
                     /locus_tag="OO309_000392"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCR5845664.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(8160..9659)
                     /gene="glcD"
                     /locus_tag="OO309_000393"
                     /pseudo
     CDS             complement(8160..9659)
                     /gene="glcD"
                     /locus_tag="OO309_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9910..10680
                     /gene="glcC"
                     /locus_tag="OO309_000394"
     CDS             9910..10680
                     /gene="glcC"
                     /locus_tag="OO309_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCR5845665.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10804..11945)
                     /locus_tag="OO309_000395"
                     /pseudo
     CDS             complement(10804..11945)
                     /locus_tag="OO309_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            12303..14033
                     /locus_tag="OO309_000396"
     CDS             12303..14033
                     /locus_tag="OO309_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCR5845666.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            14030..14944
                     /locus_tag="OO309_000397"
     CDS             14030..14944
                     /locus_tag="OO309_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR5845667.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            14977..15225
                     /locus_tag="OO309_000398"
     CDS             14977..15225
                     /locus_tag="OO309_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5845668.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            15225..16396
                     /locus_tag="OO309_000399"
                     /pseudo
     CDS             15225..16396
                     /locus_tag="OO309_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(16432..17511)
                     /gene="lptG"
                     /locus_tag="OO309_000400"
     CDS             complement(16432..17511)
                     /gene="lptG"
                     /locus_tag="OO309_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5845669.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(17508..18578)
                     /gene="lptF"
                     /locus_tag="OO309_000401"
     CDS             complement(17508..18578)
                     /gene="lptF"
                     /locus_tag="OO309_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5845670.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18609..19169)
                     /locus_tag="OO309_000402"
     CDS             complement(18609..19169)
                     /locus_tag="OO309_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5845671.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(19181..20005)
                     /locus_tag="OO309_000403"
     CDS             complement(19181..20005)
                     /locus_tag="OO309_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5845672.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(20005..21354)
                     /locus_tag="OO309_000404"
     CDS             complement(20005..21354)
                     /locus_tag="OO309_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR5845673.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(21400..22158)
                     /locus_tag="OO309_000405"
     CDS             complement(21400..22158)
                     /locus_tag="OO309_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5845674.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(22190..22903)
                     /locus_tag="OO309_000406"
     CDS             complement(22190..22903)
                     /locus_tag="OO309_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5845675.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            23077..23769
                     /locus_tag="OO309_000407"
     CDS             23077..23769
                     /locus_tag="OO309_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5845676.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23818..25317)
                     /gene="pitB"
                     /locus_tag="OO309_000408"
     CDS             complement(23818..25317)
                     /gene="pitB"
                     /locus_tag="OO309_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR5845677.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25609..27468)
                     /gene="gss"
                     /locus_tag="OO309_000409"
     CDS             complement(25609..27468)
                     /gene="gss"
                     /locus_tag="OO309_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR5845678.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27673..28539
                     /gene="yghU"
                     /locus_tag="OO309_000410"
     CDS             27673..28539
                     /gene="yghU"
                     /locus_tag="OO309_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR5845679.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28662..28910)
                     /gene="hybG"
                     /locus_tag="OO309_000411"
     CDS             complement(28662..28910)
                     /gene="hybG"
                     /locus_tag="OO309_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR5845680.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(29044..29385)
                     /gene="hypA"
                     /locus_tag="OO309_000412"
     CDS             complement(29044..29385)
                     /gene="hypA"
                     /locus_tag="OO309_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5845681.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(29378..29866)
                     /gene="hybE"
                     /locus_tag="OO309_000413"
     CDS             complement(29378..29866)
                     /gene="hybE"
                     /locus_tag="OO309_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR5845682.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29859..30353)
                     /locus_tag="OO309_000414"
     CDS             complement(29859..30353)
                     /locus_tag="OO309_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR5845683.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(30353..32056)
                     /gene="hybC"
                     /locus_tag="OO309_000415"
     CDS             complement(30353..32056)
                     /gene="hybC"
                     /locus_tag="OO309_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR5845684.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(32053..33231)
                     /gene="hybB"
                     /locus_tag="OO309_000416"
     CDS             complement(32053..33231)
                     /gene="hybB"
                     /locus_tag="OO309_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR5845685.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(33221..34207)
                     /gene="hybA"
                     /locus_tag="OO309_000417"
     CDS             complement(33221..34207)
                     /gene="hybA"
                     /locus_tag="OO309_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR5845686.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(34210..35328)
                     /gene="hybO"
                     /locus_tag="OO309_000418"
     CDS             complement(34210..35328)
                     /gene="hybO"
                     /locus_tag="OO309_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR5845687.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(35517..35804)
                     /locus_tag="OO309_000419"
     CDS             complement(35517..35804)
                     /locus_tag="OO309_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR5845688.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35923..36810)
                     /locus_tag="OO309_000420"
     CDS             complement(35923..36810)
                     /locus_tag="OO309_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5845689.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            36967..38007
                     /gene="gpr"
                     /locus_tag="OO309_000421"
     CDS             36967..38007
                     /gene="gpr"
                     /locus_tag="OO309_000421"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR5845690.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(38047..38541)
                     /locus_tag="OO309_000422"
     CDS             complement(38047..38541)
                     /locus_tag="OO309_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR5845691.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38732..39616
                     /gene="yghA"
                     /locus_tag="OO309_000423"
     CDS             38732..39616
                     /gene="yghA"
                     /locus_tag="OO309_000423"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5845692.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39888..40313)
                     /gene="exbD"
                     /locus_tag="OO309_000424"
     CDS             complement(39888..40313)
                     /gene="exbD"
                     /locus_tag="OO309_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR5845693.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(40320..41054)
                     /gene="exbB"
                     /locus_tag="OO309_000425"
     CDS             complement(40320..41054)
                     /gene="exbB"
                     /locus_tag="OO309_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5845694.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            41306..42493
                     /gene="metC"
                     /locus_tag="OO309_000426"
     CDS             41306..42493
                     /gene="metC"
                     /locus_tag="OO309_000426"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR5845695.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42633..43292
                     /locus_tag="OO309_000427"
     CDS             42633..43292
                     /locus_tag="OO309_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5845696.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(43332..44231)
                     /gene="yqhC"
                     /locus_tag="OO309_000428"
     CDS             complement(43332..44231)
                     /gene="yqhC"
                     /locus_tag="OO309_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR5845697.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            44425..45588
                     /gene="yqhD"
                     /locus_tag="OO309_000429"
     CDS             44425..45588
                     /gene="yqhD"
                     /locus_tag="OO309_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR5845698.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKQEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45693..46520
                     /gene="dkgA"
                     /locus_tag="OO309_000430"
     CDS             45693..46520
                     /gene="dkgA"
                     /locus_tag="OO309_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR5845699.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46720..47646
                     /locus_tag="OO309_000431"
     CDS             46720..47646
                     /locus_tag="OO309_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR5845700.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47697..47954
                     /gene="yqhH"
                     /locus_tag="OO309_000432"
     CDS             47697..47954
                     /gene="yqhH"
                     /locus_tag="OO309_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR5845701.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(47997..50216)
                     /locus_tag="OO309_000433"
     CDS             complement(47997..50216)
                     /locus_tag="OO309_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR5845702.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(50327..51739)
                     /gene="ftsP"
                     /locus_tag="OO309_000434"
     CDS             complement(50327..51739)
                     /gene="ftsP"
                     /locus_tag="OO309_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR5845703.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51814..52551)
                     /gene="plsC"
                     /locus_tag="OO309_000435"
                     /gene_synonym="parF"
     CDS             complement(51814..52551)
                     /gene="plsC"
                     /locus_tag="OO309_000435"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR5845704.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52785..55043)
                     /gene="parC"
                     /locus_tag="OO309_000436"
     CDS             complement(52785..55043)
                     /gene="parC"
                     /locus_tag="OO309_000436"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR5845705.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(55589..56071)
                     /locus_tag="OO309_000437"
     CDS             complement(55589..56071)
                     /locus_tag="OO309_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR5845706.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(56124..56516)
                     /gene="ygiW"
                     /locus_tag="OO309_000438"
     CDS             complement(56124..56516)
                     /gene="ygiW"
                     /locus_tag="OO309_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR5845707.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56668..57327
                     /gene="qseB"
                     /locus_tag="OO309_000439"
     CDS             56668..57327
                     /gene="qseB"
                     /locus_tag="OO309_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR5845708.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            57324..58598
                     /gene="qseC"
                     /locus_tag="OO309_000440"
     CDS             57324..58598
                     /gene="qseC"
                     /locus_tag="OO309_000440"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR5845709.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(58644..58976)
                     /locus_tag="OO309_000441"
     CDS             complement(58644..58976)
                     /locus_tag="OO309_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCR5845710.1"
                     /translation="MLKQKIKTIFEALPYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            59295..59876
                     /gene="mdaB"
                     /locus_tag="OO309_000442"
     CDS             59295..59876
                     /gene="mdaB"
                     /locus_tag="OO309_000442"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR5845711.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59907..60221
                     /locus_tag="OO309_000443"
     CDS             59907..60221
                     /locus_tag="OO309_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5845712.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(60269..62161)
                     /gene="parE"
                     /locus_tag="OO309_000444"
     CDS             complement(60269..62161)
                     /gene="parE"
                     /locus_tag="OO309_000444"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR5845713.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(62190..62771)
                     /gene="yqiA"
                     /locus_tag="OO309_000445"
     CDS             complement(62190..62771)
                     /gene="yqiA"
                     /locus_tag="OO309_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR5845714.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62771..63598)
                     /gene="cpdA"
                     /locus_tag="OO309_000446"
     CDS             complement(62771..63598)
                     /gene="cpdA"
                     /locus_tag="OO309_000446"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR5845715.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(63623..64045)
                     /locus_tag="OO309_000447"
     CDS             complement(63623..64045)
                     /locus_tag="OO309_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR5845716.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(64046..64675)
                     /gene="nudF"
                     /locus_tag="OO309_000448"
     CDS             complement(64046..64675)
                     /gene="nudF"
                     /locus_tag="OO309_000448"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR5845717.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64880..66361
                     /gene="tolC"
                     /locus_tag="OO309_000449"
     CDS             64880..66361
                     /gene="tolC"
                     /locus_tag="OO309_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR5845718.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            66509..67180
                     /locus_tag="OO309_000450"
     CDS             66509..67180
                     /locus_tag="OO309_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR5845719.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            67186..68346
                     /locus_tag="OO309_000451"
     CDS             67186..68346
                     /locus_tag="OO309_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5845720.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(68384..69172)
                     /gene="ygiD"
                     /locus_tag="OO309_000452"
     CDS             complement(68384..69172)
                     /gene="ygiD"
                     /locus_tag="OO309_000452"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR5845721.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            69315..70088
                     /gene="zupT"
                     /locus_tag="OO309_000453"
     CDS             69315..70088
                     /gene="zupT"
                     /locus_tag="OO309_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR5845722.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(70146..70316)
                     /locus_tag="OO309_000454"
     CDS             complement(70146..70316)
                     /locus_tag="OO309_000454"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5845723.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREEIAEKVE"
     gene            complement(70577..71230)
                     /gene="ribB"
                     /locus_tag="OO309_000455"
     CDS             complement(70577..71230)
                     /gene="ribB"
                     /locus_tag="OO309_000455"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR5845724.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(71334..71482)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            71604..71894
                     /gene="ubiK"
                     /locus_tag="OO309_000456"
     CDS             71604..71894
                     /gene="ubiK"
                     /locus_tag="OO309_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR5845725.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        18022 a        19160 c        17737 g        17138 t
ORIGIN      
        1 ttacgtaccg tttaaacaaa gacaatacgt ctctttattt ctcataaaac cccaatccct
       61 tacagggctt attgaaaaat ttaaaaatca caaatttctc aatactcgaa atgagatcac
      121 ataagaaatc aaaaagcctt ctcatctatt ttaagttttc ccaacggaaa ataagagctt
      181 tcattaatac tgaaccactt tactgttcta tttgttcagg tattgcatta cggtgtgctg
      241 agatccatcg gtgcatccct gtatcccgcg atgagactaa aatagtcgtg atgtaagctc
      301 gtatcagatt cttagtgaat accacatttg ttataattaa gtgaaaagat acattgctga
      361 tttacattat ttggtttaca aaatgtcatc acttgcagag ttatcgctga cagatacctt
      421 taatctctta ctactttcgt gttattgcaa tacaaccatc cgtaaagcag ggctattttt
      481 agcaggaaat actcaggtgt caacattcga actccaactc tgaaatattc atataagtta
      541 atgattttaa tgattttaat gattttaatg attttaatga ttttttcatt tattcacacc
      601 taataaaagt attcaaattc agatgatttt tctgctcaac agacacaaac aggcaataat
      661 actctccaaa gcgaacaata tttggcatta cgatttaatg cttcataatc accaaattat
      721 ttaaccataa attttcttat ttctaacaaa atgattggtg tgttgttgga gttaaaatac
      781 atacataata ttaacatctg ccaacatttc actttccgca agacggatga tttcgaatcc
      841 tcatccacgt taccctatcc caaatatctt tattaatagg cagtaacgaa cactgttata
      901 gcaagattaa cttattaacc taacggcatc ttttatgtaa caagtggaga taaattgctt
      961 aaaattgatc ctcgacaaat cttaaaagtt ctcattattc tgcaaaatca gctttgcgaa
     1021 taatcttatg tttttgggag ttttgggaat tttgatctac tcattgttct agtgaaaaat
     1081 ataaataaga gaaaaaacgc cattcgtcat gaaagcaaca gatgaagcaa tgtaataaca
     1141 tactaaaatt gatctcatga aagtgggtac tttataacca gcacagaagc caagttgtat
     1201 aaatattaaa caattcgcat cgtaaaacgg agactacgta taaagaagcc aatcgtaaaa
     1261 acggttccct ggcgagataa acgacagaca aatggaaagc cgggtgatat ttaccttcat
     1321 ccggctttat tgatttacga gactaacatc ccggtaaaca catacgcctg cagcagggtg
     1381 ataacgctgg caaaaatcag actgtgcttc acggtgtagc ggaacagttc agattctcgg
     1441 cccaccatgc ccgtcgcggc gcaggccacg gcgatagatt gcggggagat catcttgcca
     1501 gttacgccgc cgctggtatt tgctgccacc agcagagtgt cagagacgtt gatttgctgc
     1561 gccgtggtcg attgcagtga accaaacagg gcgtttgaag aagtgtccga cccggtaagg
     1621 aatacgccca gccagccgag aaacggtgag aaaaacggga acatcacgcc tgtacctgcc
     1681 agtaccagcg ccagcgtggt ggacatgccg gaatagttgg tgacgaaggc gaatgccagc
     1741 accatgccaa tcgacaatat cggccacttc agactaatta gcatttcggc aaagacgcca
     1801 atccctttct tgatgcccac gccgaggatg aagatagaga taatcgccgc aataaaaata
     1861 gcggtgccgc cagccgagag agggtcgaat ttaaacaccg catccattgg cgttggttgg
     1921 gcgacaatgg gtgccgcttt caacacttgt tgatgcaaat gagggatctg gaaattaatc
     1981 accagtgaat aaaacgcgcc gcccggagca aataacgctt taaacggctt catggtccag
     2041 atggtgacca gcaccgttaa gattaaaaac ggtgaccacg cacgaatgat ttgccccaga
     2101 ctatattctg aaggcacggg accgccagaa gatggcttat ttaccaccat cgcacctgcg
     2161 gattgcccca tgctgattgc cgtttcggta tttttcggcc gccagacttt aaggaataaa
     2221 gcgagtgaga cgatactcac cagcgccgaa gtaatatccg gcagttccgg accaatatag
     2281 ttagaggtaa agaactgagt gacagcgaag cttcccccag caaccagcgc cgctggccac
     2341 gtctctttca cccctttcca gccgtccatc attgctacca gccagaacgg cacaagaacc
     2401 gacaggaacg gtaactgacg tcccgccatt gcgccaatgt ggaacggatc gattcccgtt
     2461 acctgaccgg cgaccagaat cggcacgccc aacgcaccaa acgccaccgt cgcaccaaag
     2521 ccagcagctc cttccagcaa cgcaccaaag gagaaaccaa tcagtaacac ctgcaaacgc
     2581 tgatcgtcgg tgatggagat aaccgagctg cggataatat cgaactgccc gctggcaacg
     2641 gttaatttat acaggaacac cgccgcgaca ataatccacg caattggcca taatccataa
     2701 ataaagccat agcccgcagc agcaaatgcc atatcaatcg gcattttaaa ggcgaatatc
     2761 gcaatcagga tagataatat aagggttatt gctccagcaa catgtccttt cagacgtaat
     2821 accgcgagtg caacgaagaa gaatattatc gggatcaggg cgaccagagc ggatagcccc
     2881 agtcctccca tcggcatata catttgggtc caggtaacca tattgttcag tctcttatta
     2941 tctttatatg cttgatatac ttaaggttgt aataagcaaa agaggactga actgtaaaat
     3001 ataggcgtta tactttacag caacagtacg ccgctaacgc aattgctacc tctggcataa
     3061 caagtatatc gggtaagggt ttctgttccg cacacgcagg cgcagagtat cgttaagatg
     3121 tccatattgt tgttttaggc ccgctagtaa tgcgctacgg gtatttaata ttgttaaacc
     3181 ctgataatcg ctccggttat ttccgggata aatgtactac cgcagttact atcatagccc
     3241 cgacaataaa acttgccggg gcttttttga cgctattaat gactttcttt ttcgcgtaaa
     3301 cgccaggcgt gtaataacgg ttcggtataa ccgtttggct gtttcacgcc gaggaagatt
     3361 aaatcgctgg cagctttaaa agcacacgag ttagcgaaat tccccgccat cggacgataa
     3421 gccggatcgc cagcattttg ctgatcaacc actttcgcca tattctccag cgacgcctgc
     3481 acctgttctt tggtcagaat accgtgacgt aaccagttgg cgatatgctg gctggagata
     3541 cgcagcgttg cgcggtcttc catcagcgcc acgttgtgaa tatccggcac ttttgaacaa
     3601 ccaatcccct gctccaccca acgcaccacg taccccagaa tcccctgcac gttgttatcc
     3661 agctcttgtt ggatctcttg cgccgaccag ttagcgtttt cagccaccgg aatagtcagc
     3721 agatcgtcca gcagcggttc aaattcagca ctgaactcgg tctgggcaat gttggcttgc
     3781 acgctctgta cgttggtatg gtggtagtgc agcgcatgga gcgtagcagc ggttggtgac
     3841 ggaacccagg ctgtgtttgc cccggcacgc agttggtcgc ccttctggct gtacatgtct
     3901 gccatcaggt ccggcattgc ccacatgcct ttaccgattt gcgctttacc gcgcagccca
     3961 cagaacagac cggaaagcac gttattacgt tcgtaggctt tgatccacgg cgtcgatttc
     4021 atctggtttt tacgcagcat cgggccagct tccatcaccg aatgcatttc atcgccggta
     4081 cggtcgagga aaccggtatt gatgaacgcc acgcggttgc gcgcctgagc gatacagcta
     4141 cgcaagttca gcgaggtccg gcgttcttca tccataatgc ccattttcag ggtattcggt
     4201 gccataccga gcattgtctc aatgcgggta aacagtttgt tggcgaacgc cacttcctgc
     4261 ggaccgtgca ttttcggttt cacaatatag acgctgccag tgcgcgagtt tttctgcact
     4321 tttaaatcat agagggcaat cgcgccagtc atgacgccat caagaatacc ttccgggatt
     4381 tcattgcctt cgctgtccca aatcacagga atggtcatca aatgacccac gttgcggata
     4441 aacagcaacg agcgtccgtg cagagaaatt tcagagccat cggcggcggt gtactgacga
     4501 tcgtcattca gtttacgtac gatttgccga ccgtttttct ccattttctc ttgcagagtc
     4561 ccctgcatca ggcccagcag attgcggtac agcaggattt tatcttccgc atcaaccgcc
     4621 gcgaccgaat cttcgcagtc gagaatggta ctgatagcag cttcgacgat aacatcgttg
     4681 atgtgcgccg gatcgtcttt gccaatccgc ccgttggcat caatttgcag ttcaatatgc
     4741 aggccgttat ttttcagcaa aatgcaggtc ggcgcagcgg catcgccacg gtaaccgaca
     4801 aactgtgctg gagtacgtaa cgtggtttct ttaccatttt tcaactggat gcgtaattgt
     4861 ttatcgacca ccttaaacgc caccacatcc tgatagctgc cgttttccag cggtagtgat
     4921 tcatcgagga aacgtcgaac ccaggcgata acctgctcac cgcgctgcgg atcgtagcca
     4981 ctgaccatcg ccccttcctg cgggatgatg tcgctgccgt ataacgcatc gtacagtgag
     5041 ccccagcgag cgttcgccgc gttcagcgcg tagcgggcgt tcattgccgg aaccaccagc
     5101 tgtggccccg cctgactggt gatttcgctg tcaatgcccg tggtttccac cgtcacgcgc
     5161 tccggttgcg gcaccaggta gcccagttca cgaaggaaag atttataggc cgctttatct
     5221 tttaccggcc ccggattgct gcgatgccac tcatcaagcg ccgcctgaat gcgatcgcgt
     5281 tctgccagca actgacgatt ttctggtgcc agatcgtgaa cgatctcatc aacattgtgc
     5341 cagaatgcag cagcgtccag ttccacgccc ggcaaaactt ctccatccac aaaacgttta
     5401 aaattggcgt caatgcgtaa acggccctgg gttatggttt gactcattgt ttctctcctc
     5461 gttttcgctt atttcgccaa caccgctgct gccactttcg cgacctgcgc atcctgtgct
     5521 ccggttaaac cagaaacgcc cacggcacca ataatttgcc catcaacaac aaccggtacg
     5581 ccgccttcca gcgacgtcag taacggcgca gtcacgaacg cggtacgtcc gttgttcacc
     5641 atctcttcat agcccttagt ttcacgacgc cccagcgcgg cggtacgcgc tttctcctgg
     5701 gagatgtaag ccgcaatcgg cgcgcaatcg tccatgcgac ttaacgccag cagatgaccg
     5761 ccgtcatcgg caacagcaat ggaaacagac cagttatttt tctgcgcctc ttcctgacct
     5821 gcggcaataa ttgcactcgc catttgctgg ctaagaatga ctttagtttt cattttttta
     5881 ttccttttca agggcttgtt ctacaatttc aatccagtga cgcacagagg tacgaccggc
     5941 gctcgccaga tgcgtctggc aaccaatgtt ggcggtgacg atcatttccg gtttgccgct
     6001 ttccagcgca ttcattttgt tatcccgcag ctggcgtgcc agatcgggat gcgttaacgc
     6061 atatgttccc gctgaaccgc agcacagatg gctgtcggga acgtccgtta aggtaaatcc
     6121 aagacgaagt aacacttttt ccacttcgcc gttcagcttt tgcgcatgtt gtagggtaca
     6181 cgggcagtgg aaggccagtt ttttatcgcc gcgaattgcc agtttttcca gcggttcctc
     6241 gcgcagaagt tcgactaaat cgaccgccag ttcactgacc tgacgcgctt tatcggcata
     6301 taacgcatcg tttttcagca tctgcccata ctctttgacg aacgcgccgc aaccgctggc
     6361 ggtttgcaaa attgcctcgg cacctgcttc aatcgcgggc caccaggcat caatattatt
     6421 gcgcgcccgt gccaaccctt tctcctgcgc attaagatga tagtccaccg cgccacaaca
     6481 gcctgcttcg ttagctgaca tgacgctgat ccccagacga tccagcactc gcgcagttgc
     6541 cgcgttggtg ttgggcgaaa gcgtaggctg ggcgcagcct tccagcatta aaacccgacg
     6601 cttatggcgc agcggcggac gcggtttagc tttcaccgtt tcagcaggca gttttgctct
     6661 gacctgttcc ggtaaaaacg gtcgcagcac cagccctacc tgcgtcagcg cacggaagac
     6721 cgccggacgc ggcactacct ggcgcaatcc ttcgcgcagc attcgctccg gcagtgggcg
     6781 tttcactttc tgctcgacaa tatcacgccc gatatccagc aaattgtgat agcgcacacc
     6841 agaaggacag gtggtttcac aattgcggca agtgaggcag cgatcgagat gctcctgtgt
     6901 tttaagcgtg acttcgttgc cttccagcac ctgtttaatc agatagatgc gcccgcgcgg
     6961 cccgtccagt tcatcgccca gaagctgata ggttgggcag gttgcggtac aaaatccgca
     7021 gtgaacacag gcgcacagga tgctgtcggc ttccagcgcg cgttctgccg catctcttcg
     7081 gttaatcggg tttgcatagc ctgctcctca aagttccgcg tacatgcgac cggggttaaa
     7141 cacgccgcaa gggtcgagct gctgtttaag ctgctggtga tagcggaata aaggagccga
     7201 tagcggggca aagccaccat ctccggcact aaagcgggtc gcatgaccgc cagcgttgcg
     7261 ggcgatgcga tggatttgat tgtcatcggc tgtcgatttc agccagcgta acgccccgcc
     7321 ccagtcgatc agttgctcgc cgggtaaatc catcatcggc gcatcgctgg gtaatgaaat
     7381 gcgccataag gtacctggta acgagaagaa cggcagttgt tgttcacgca attgctgcca
     7441 gaactgaccc acaacctctt cgccacccag cagttcacgc gctgctttta ccgatccttc
     7501 gccgccctca aggcggatcc acaacgcatt gtcgaagtaa cataagccac taatgggtaa
     7561 tggctggagt tgccactcgg cgatttcact catggcttct tgcaggctga tttcccgacg
     7621 caggctcaga gaggcacgcg gtcgcggtaa cactttcatt gagatttcag tgagcacgcc
     7681 aagacaaccg tagcttccgg ccattaaccg tgagagatcg tatccggcaa cgtttttcat
     7741 cacttcgcca ccaaaacgca gatgttttcc agtgccggta atgatgcgcg tgccgaggac
     7801 aaaatcgcgg accgaaccgc tccacgggcg acgcggcccc gccagcccgc aggcgaccat
     7861 cccgccccag gtggcttctt caccataatg cggcggctcg cagggaagca tttgcccggc
     7921 gctctccagc gccgcttcaa tcgccaccag cggcgttccg gcacgagcgg ttatcaccag
     7981 ctcggtcggg tcgtaattaa caatgccgcg atgacaacgc acatccagcg tttgcccggt
     8041 gactgggcga cctaaaaagg ctttgctatt gctgccctga atcaccagcg gcgttttatc
     8101 gctaattgcc tgattcacct gctccagcag cgcctgactg taatcacact cgcgtagcat
     8161 caaaagcgct ccagttcagg aaaaggtaaa tgaccgtgat gcacatgcat ggcaccaaat
     8221 tcagcacagc ggtgtagcgt gggaatgttt ttcccagggt tcagcaaacc atcggggtca
     8281 aacgccgcct tgaccgcatg gaaggtcgtg atttcatcgc tgttgaactg ggcgcacatt
     8341 tgattgattt tttctcgccc gatgccatgt tccccactga tgctgccgcc aacttcaacg
     8401 cagagttcga ggatcttccc gcccagctct tccgcgcggg caaattcacc aggttcgttg
     8461 gcatcgaaaa ggattaacgg gtgcatgttg ccatctccgg catgaaagac gttggcaaca
     8521 cgtaaatcat attgctgcga taaacgggca atgccttcca gtacgccagg cagggcgcga
     8581 cgcgggatgg tgccatccat gcagtagtaa tccggggaga tacgtcctac cgccgggaac
     8641 gcatttttgc gaccggccca gaaacgtacg cgctctgctt cgtcctgtgc caaacggacg
     8701 tcagtcgcgc ccgctttcaa caagatgtag ttaacccgct cgcagtcttc ctgtacgtca
     8761 gactccacgc cgtccagttc gcataacaaa atcgcttcgg cgtcgacggg ataaccggca
     8821 tgaataaaat cttccgccgc gcggatcgac aggttatcca tcatctccag cccgccgggg
     8881 ataatgccat tggcgatgat gtcaccaacc gcaagtccgg ctttttctac cgagtcaaag
     8941 ctggctaaca gaacccgcgc cacgggcggc ttcggcagca gttttaccgt cacttcggtg
     9001 gtcacgccga gcataccttc cgatccggtg aacagcgcca gcaggtcaaa accaggtgaa
     9061 tccagcgcgt ccgatcccag cgtcagcgcc tcgccgtcca gcgtttgcac ttcaattttc
     9121 agcaggttat gtacggtcag accatatttc aggcagtgga cgccaccggc attttccgcc
     9181 acattgccgc caattgaaca ggcgatttgt gaggaaggat ccggcgcgta atagagatta
     9241 tgcggcgcaa cggcctagga gatcgccagg ttacatacgc ccggctgcac gcgcgcgcgg
     9301 cgaccaacgg ggttaatgtc gaggctctct ttaaagcgcg ccatcaccaa caacacacct
     9361 ttttccagcg gcagcgcgcc accagaaagc ccggtgcctg caccacgggt caccaccggt
     9421 acacgcaggc gatggcagac agccagaatc gctgtcacct gttccatttg cttaggcaga
     9481 acaaccagta atggacgcgt gcgatacgcg ctcaacccgt cacactcgta aggaatgatc
     9541 tcctcatcgg tatgcaggat ctcaagtcca gggacatgct cacgcagtgc catcagtacc
     9601 gatgtgcggt cgacatcggg taaagcgcca tcaagacgct cttcgtacaa gatgctcatg
     9661 agtaggcttc gctttgttgt gttgtgtggc agctgatttt tgcgcgctgc ttctgtgaac
     9721 agttattaag cgggcttttc gttttcgtct atctctttag ctaccggtca gaccattttt
     9781 tttccagctc tgtgaccttg tcttggttaa cttaatgtta aattgatgta acataatcac
     9841 ttgcgtgatg tgcgtgtttt gcgagttaag aacagaaaaa ttggtcctac ctgtgcacga
     9901 ggtccgggaa tgaaagatga acgtcgccct atttgcgaag tggttgcaga gagtatcgaa
     9961 cggttaatta tcgacggcgt actgaaggtc ggtcagccgc ttccctcgga acgtcgactg
    10021 tgtgaaaagc tcggcttctc acgctccgca ctgcgtgaag ggctgaccgt gctgcgcggg
    10081 cgcgggatta ttgaaacggc gcagggtcgc gattctcatg tcgcacggct taatcgggag
    10141 caggacacca gcccgctgat ccatctgttc agtacgcagc cgcgaacgct gtacgatctg
    10201 ctcgacgttc gcgcattact ggagggcgaa tcggcaaggc tggcggcaac gctgggaacg
    10261 caggctgatt ttgttgtgat aacccgctgt tatgaaaaaa tgctcgccgc cagtgagaac
    10321 cacaaagaga tttcgctgat cgaacatgcg cagttggatc acgctttcca tctcgccatt
    10381 tgtcaggctt ctcacaatca ggtgctggtg tttacgctgc aatcattgac cgatctgatg
    10441 tttaattcag tgtttgccag cgtaaataat ctctaccatc gaccacagca aaaaaagcag
    10501 atcgatcgcc agcatgcgcg gatctacaac gcggtgttgc agcggctgcc gcacgtcgct
    10561 cagcgcgcag cgcgcgatca tgtacggacc gtgaaaaaga atctccacga tatcgagctg
    10621 gaaggccacc atttgattcg ctcggcggtg ccgctggaga tgaacaaggt gggaatgtga
    10681 gcagagtttt accctatccc caaggcgagg ggaccgcccc tgcacttaac aggatctggt
    10741 gttaaatctg ttcatctata ccctctcccg aatgaccgcc cctgcgctaa acaggatctg
    10801 gtattaaatt tgcttttcat acaatcggta acgcttgtac ggctccgccc caatgcgttc
    10861 cagcatgtta ttcatgcctg tattggtttc gaggatccat gacatctcca gcgcatcgat
    10921 cttccggcgg gcaaacggat cgcgtaaggc ttcaatcaat aacagcgcaa tcaccgggcc
    10981 gatgcggctg aactgatact cgtcgcgcac gcccatcagc ggtactcgcg cagttcgcac
    11041 accgctgact ttcaaacgcc acagcaattt tgcccagccg aagggaaaga gcgatccgtt
    11101 cagatcggca atcgcctcgt tgatgttagg caagccacaa taaacgcgca gggtgcagaa
    11161 tcaatctcag cgatgtagat catatcgtcc ggcaccagat atttaagttg gtcgcccatg
    11221 gtcgcgaatt catgttcggt aaacggcaca aatccccagt tgtgctgcca gccagagttg
    11281 aaaatctcac gcaggatctg catctcttcg gcaaaccgct gacgattgat gcagcgaatg
    11341 gtcacctttt tgcgcacctg atccatcagt ttttttagcg ccggagagaa agtgagatcg
    11401 gttcgctgca tccaccacgc cagtaaatca atgcctttgt gataacccag ttgttcaata
    11461 tgcgcggcat accacggttt gccgtgtggc atcatcgcac agggtggtgt gtcaaaacct
    11521 tcaatcagta atccgctttc ctgattgata ttcaggctga aaggcccgct gatcttactt
    11581 gcaccttgtg acttcaacca cgcttccgct gcgccaaaca acgcggcaaa atcctgcgga
    11641 tcatcaatgg cgtcaatcat gccgaaatga ccggtatctt caccgtaacg ctcacggtgc
    11701 aaggtatcta tttgcgcggt aatacgccca actatctgcc ccgctttttt tgccacccac
    11761 gcctgccaga tgatatggtc cgtccccgga tttttcgcag acaaatgctc gttgcgttca
    11821 atgaataaag ggggtatcca gtttggatcg tcgggataaa gtgatgacgg aaaagcgata
    11881 aatgccttaa ggtcattttt attaattact ttttcaattt taatcatcag cagatcgcat
    11941 tccatatctc tgtggataac aaataattaa tggtgaaaaa caggttgcaa cagcaacgag
    12001 cggcatctta ataatcaact caatgaatac aaacgacgaa taaatagtat taattaatga
    12061 ttatgataag gtcagaggct acacagaaac gcctcaatgc cttttgtcaa tttaaggcga
    12121 ccggacaagg cttcttctta atatattcat aaattaaata tcattcataa taaaaaaaac
    12181 ttaaactcat tatttaaatc aatgttcatt ttcacaccga tgaaaatact atctcattta
    12241 tcttttatgc ctatataatc caccgcatta ttgtcatttt atttttgata tccaattttt
    12301 tcgtggtgta tatgtctaat aaaatcttta cgcattccct acctatgcgc tatgccgatt
    12361 ttccaacgct ggttgatgct ttggactacg ccgctctgag tagagccgga atgaattttt
    12421 atgacagacg ttgccaactt gaagatcaac tggaatatca gacattaaaa acgcgtgccg
    12481 aagctggtgc gaagcggttg ttatcgctga acctgaaaaa aggcgaacgc gtggcgctga
    12541 ttgccgaaac gagtagcggg tttgtagagg ctttttttgc ctgtcagtat gctggcttag
    12601 tcgccgtccc gttggcgatt ccaatgggcg tcggtcagcg ggattcctgg agcgccaaac
    12661 tccagggttt actggcaagt tgccagcccg cagccattat cactggtgat gagtggttgc
    12721 cactggtcaa tgccgcgacg cataacaacc ccgaattaca tgttttaagc cacgcttggt
    12781 ttaaggcatt accggaagcc gatgttgcgc tccagcgtcc agttccgaac gatatcgcct
    12841 acctccagta cacctccggc agcacccgtt ttccccgtgg cgtcattatc acccatcgcg
    12901 aagtgatggc taatctacgt gctataagcc acgatggcat taaattacgc cctagcgacc
    12961 gctgcgtctc ctggcagcct ttctaccatg atatgggact ggtcggcttt ctcctgcccc
    13021 ccgtcgccac gcaactttca gtagattatt tgcgcactca ggattttgcc atgcgtcctt
    13081 tgcaatggct taaattgatc agtaaaaatc gcggcaccgt ttccgttgcg ccgccgtttg
    13141 gctatgaatt gtgccaacgc cgcgtgaatg aaaaagatct cgctgaactg gatctttcct
    13201 gctggcgcgt cgctggtatt ggtgctgagc cgatctccgc agaacaactc catcaattcg
    13261 ctgaatgttt ccgtcaggtt aactttgaca ataaaacttt catgccgtgc tacggactgg
    13321 cagaaaatgc gctggctgtc agcttctctg atgaagcctc cggggttgtg gttaacgaag
    13381 tggatcgcga catcctcgaa tatcagggta aagccgtcgc gccgggtgca gagacacgcg
    13441 ccgtatcgac tttcgtcaac tgcggcaaag cgttgccgga acatggtatt gaaatccgca
    13501 atgaagcagg tatgccggtc gcggaacgtg tggtaggcca tatttgcatc tccggtccca
    13561 gtctgatgag cggttacttt ggcgaccagg tttcgcaaga cgagattgcc gcgacgggct
    13621 ggttagacac cggcgacctc ggttatctgc tggacggtta tctgtatgtc accggacgca
    13681 ttaaagatct gattattatt cgtggccgta atatctggcc gcaggatatt gaatatatag
    13741 cggaacagga accggaaatt cattctggcg atgcgattgc ttttgttacc gcccaggaaa
    13801 aaatcatttt gcagatccag tgtcggatca gcgacgaaga acgtcgcggg cagcttatcc
    13861 acgcgctggc ggcacggatc caaagcgaat ttggcgtgac cgcggctatc gatctgttgc
    13921 cgccccacag tattccccga acgtcctccg gcaagcctgc ccgtgcggaa gcgaaaaaac
    13981 gttatcagaa ggcttatgct gccagtctta atgtgcagga atccctggca taaatcaaac
    14041 tgtcgcggtg acgggcgcta ccgggtttat cggtaaatat attatcgata acctgctcgc
    14101 ccgcggcttt cacgttcgcg cattgacgcg tactgcccgc gctcacgtca atgataatct
    14161 cacctgggtg cgcggttcgc tggaagatac tcattcactt agcgagctgg ttgccggagc
    14221 cagcgtagtg gtccattgcg ccgggcaagt gcgcgggcac aaagaagaga ttttcacccg
    14281 ctgtaacgtt gacggcagcc tgcgcctgat gcaagcagca aaagagagcg gcttttgcca
    14341 acgttttctg tttatctctt cgctggcggc gcgccatccc gagctctcct ggtatgcaaa
    14401 atccaaatac gtcgccgagc aacgactggc agcaatggcg gacgaaatta cgctgggcgt
    14461 ttttcgcccg acagccgtgt atggtcccgg cgataaagag ttaaaaccgc tgtttgactg
    14521 gatgctacgc ggcctgctgc cacgacttgg tgcgccagat acacaactct cttttcttca
    14581 cgtcaccgat tttgcgcaag cagtgggtca gtggttaagc gccgaaactg tacagacgca
    14641 aacctatgaa ttatgcgatg gcgtcgccgg tggctatgac tggcaacgcg tacgacagct
    14701 tgccgccaaa gcccgttgtg gttccgtgcg aatggttgac attcctctgc cggtactcac
    14761 aggccttgcg gatatcagta ccgcgttgag tcgcctggca ggtaaagaac ctatgctgac
    14821 ccgctcgaaa attcgtgaat taacccacgc cgactggtcg gcaagtaata accgtatttc
    14881 tgaagatatc aattggtttc ccgggattag cctggaacac gcattacgca acgggctatt
    14941 ttgattgtct tgttttaaag gtgtgttaac gtttctatgg taaatcgtga aatagtaatg
    15001 gattacatcc tttcctgttt acaggatttg gtcgaaaacg gtgtggaaat taagcccgac
    15061 agcgatctcg tcaacgatct tggcctggag tcgatcaaag tgatggatct cttaatgatg
    15121 ctggaagaca gattcgatat ttccattcct attaatattc tgctggatgt caaaacccct
    15181 gcgcaattaa tggaaactct acttccatgg ctggagaata aataatgggg ctatacgata
    15241 aatatgcgcg ccttgctggc gagcgtctgc aatttagtga caacgggtta acaccgtttg
    15301 gcacctgtat tgatgaagtg tattccgcca ccgaaggccg gatcggcaat aaaaaagtga
    15361 ttctggccgg gaccaataat tatctcggtt taacctttaa tcatgatgcc atcagcgaag
    15421 gtcaggccgc gttagcggcc caaggcaccg gcaccaccgg ttctcgtatg gcaaacggca
    15481 gttatgcgcc gcacctggcg ttagagaaag agattgccga gtttttcaat cgccctaccg
    15541 ctatcgtttt tcaaccggtt ataccgctaa tttaggcgtc atcagcgcac ttgccgacca
    15601 taacgccgtg gtgttactgg atgccgacag ccacgccagt atttatgacg tctgttctct
    15661 cggtggcgcg gaaattattc gtttccgcca taacgatgct aaagatctgg aacggcgcat
    15721 ggttcgcctc ggtgaacggg cgaaagaggc gattatcatc gttgagggca tctacagcat
    15781 gctcggtgac gttgcgccgc tggcggaaat tgttgatatc aaacgtcgtc tcggtggtta
    15841 tttgattgtc gatgaagcgc attcgtttgg cgtgttaggc gcaacggggc gcggactggc
    15901 tgaagctgtc ggcgttgaag atgatgtgga tattatcgtt ggtactttca gtaaaagtct
    15961 ggcctcgatt ggcggttttg ctgtcgtttc ggaagcgatg gaagtgctgc gctacggcag
    16021 ccgcccgtat atttttaccg cctctccttc tccgtcctgc attgcgaccg tgcgctcgtc
    16081 gttaagaact attgccacac aaccagagct gcggcaaaag ctgatggata acgccaacca
    16141 tctgtatgac ggtctgcaaa aactgggtta tgagttaagt tcacatatta gcccggtagt
    16201 gccagtaata atcggttcga aagaggaagg tttacgcatc tggcgcgagc ttatctcgcc
    16261 gggcgtgtat gtgaatctta tcctgccgcc tgccgcgcct gcaggcatca cgctgctgcg
    16321 ctgtagcgta aatgctgccc acagtcgtga gcaaatcgat gccatcattc aggcatttgc
    16381 tactctgaag cagtaatgtg agcccgccgt ggtgtttgcg ccacggcggg tttaatgttg
    16441 tctgtcatac caatacacca ggctcaacgc caccaacaca aacgctaccg gtaaaccgag
    16501 cgcggtaaca ggtgcgctta acgcaaatag caatcccaga ttaaccatga tttgatagct
    16561 gatccaggtg agtaagccca cgatgacgcc tacagcgaga cggctaccca tcccaggcga
    16621 acgcggggca ctaaaggtaa acggcacagc cagcaaaatc atcgccaggg tcaggatggg
    16681 ttgccccagt ttttcccaca gtgcaaggtg gtattcgctg ctgggttgcc cggtttcctg
    16741 caaataatgg atgtaatgat taagctgacg gacagaaaaa gtattccccg gcattgataa
    16801 ctcttcaaga ttcatactgg tgaaggccga ttgccaggca agattatcta atgtttccag
    16861 cgtttctttg ccattcagcc attttttatg attcacacca tgcaggaccc acgttttgtc
    16921 gtcttcaatg gtggcactgc gtgcataaat gtaggattca agcgagagat cgtcgcgata
    16981 atgaaatatc tccacgccca ccggctggcc ctgctcgttc agcgatttca ccgtcacaaa
    17041 ttcattacca cgcctggccc acagcatatt gccggtaatg tcatcgtcct cccccaacgc
    17101 ggtggcggtc gatttgattt gcaacgcctg ttgctgcaat ggcgacgcca cccactcatc
    17161 tatcgcgcct aacgaaacag tccacaatat cccggcaacc agcgccacca gtgcaatacg
    17221 gaagatagaa aaccccgtgc tgcgaatggc ggtaagctca ctgtttttcg ataactgccc
    17281 cagtccaaca ataccaccca ataacgcgat aaacggcgaa agttcgacca gtgtgcgcgg
    17341 taaggtcatt agcaccacca gcaccgcctg agtccagcga taaccgcccg ggctgacacc
    17401 atccagttcg ttaatcaggt tgaaggtggt aaaaagcggc agcaatagcc ctgcagcggc
    17461 ggcaaaaccg agaaagagat gacggattaa atagcgactg aaaacattca tcgggaaaat
    17521 ttctgcagca aagagaagtc acgtgcgacc agtatcagca ggcccattag catgagtccg
    17581 ggcactaacc acaaaccagg aatgagggga atcgcgccgt tagccaccag tgtacggcag
    17641 acgtcgccgc cataaaaaat ggcaataaac agcaacgtca gtggcaataa cgttgaaaat
    17701 cgcccttgcc gcggttttac ccgacttaat gaaatggcca gcagcgccat caacaatgcg
    17761 gtcaggccac ggctttgtcg ccattgcagt tcggcatgat cggcaggaaa ggcggagcgc
    17821 gccagctccg tgaccgattt cgctttgcgt ttgacgttcg ggctttgatc cagcggattc
    17881 agatgtaatt gcagattacg gtagatctgt tcgttgtcgt cacgcccctg atgatcgaga
    17941 agataggcag tgccgttgtg caacataacg gtcggtttct cgggtgatgg gtcaaccaca
    18001 tcaaccgaac cggcgcggaa aatacgggtt cgattggcag tagaagtata aatcagcgcg
    18061 tcagtcaggc gattattatc ctgatcaacc gtctgcgaaa ggatcattcg tccgttatcg
    18121 ttagtgttaa atttctttgc ccgcaactgg cgaacatcca gctccgactg cgactgctgc
    18181 tccagttgat aaatctgcgc ataagcccac ggacgaccat acatcgacag aagggtgaca
    18241 aaaatactta gcggtatcgc cagataaaga acggctttgt acaagcgtcc cggactgccc
    18301 cccgccgcag agatagcggt aatttccgag tcggtataca tctgccctag cgtcacgcca
    18361 actgacacat acagaccaac aggtaacaac atctccagtg caatcagtac tttgtagaaa
    18421 acgatatcca gcacaacatc aagagctaat gtcccatttg ccgcttcggt cagataacgc
    18481 tgtgcggagt agctggcgaa aataaagatc agaaaaccga cgataatcag caccagacgg
    18541 cgcgtaccac gcatgatgta gtgttcaacc agcttcatct cactaacgcc ctcaatcctt
    18601 tgagtttttt attcattgat cagtttctgc gccaacgcaa tgacatgatc tacctcttcc
    18661 ggcgtcaggc tgccgtcctt tgcccactga atttgcccgg ctttattcag caccagaatg
    18721 gttgaactct gctccggcaa gcgccaggca acgcgtccct gtccattgcc gtcaataacg
    18781 aattgcgccc acggatagcg gcgtttattt ttttcgattt tgccaacaac aaaatagcca
    18841 gtaccaaaga tggcatcatc ggtgttcaca atggtggtgg gctgaaaacg atcctgcgga
    18901 aagtttgccg cttcaaccgc tttaattaac agtgagttct ttttcttcgc cgacttacgc
    18961 ccggcgatat actgaataat gcggactttt cccgctaatt gtgtgctgtt ccacttttgg
    19021 tagtgaattt cgtcgtcgct attcagaagc agctcgccgc cttcctggat ataaacaggg
    19081 gtaaccgttt tgccagtgac gaaattgtgt gcctgcacac ccggggctaa gagcatgata
    19141 atcaacgcaa taatcaaacg agatgacatc attgctccgg ctatagcgga aaacgcgaaa
    19201 tcagcagctc taagcgctga caaatatcgc tatccagcgc caacggtgca taatacgact
    19261 ggcggttttc catatcatcc gccaccatgt cgtcgtgttt atctcccatc aggccattaa
    19321 tggcggcgag gaaacgggaa atctgtaacg cctctttatt ttcaggatca agcgatctta
    19381 aggtgtcgta ataagaaacc tgctggctga tgatttttcg cacatcatga aattccctgc
    19441 cggttagtcg cggtttggca ataatctctt tcagggtatg catttgcttt tgccgatagg
    19501 caataaaact gcttacgctt tctaactggc tatggcgtaa tgaatagttc accaacgacc
    19561 agaccggaga gcttaaataa atccgtaaca ggttgccgta atacgacaca atatttttct
    19621 taccgttacg aaagccgtcc tgaaaacgcc ccagaaaaac ggtcgttttg ccgaataaaa
    19681 atccggcctg atgcttcttt aaatacagcc gttgcgcaaa acgcaggtgc ttgtagcgcg
    19741 ctcgcatgta tttaaattgt ttgcgctcat cagcagtcag ctcatcgcct ttggcttgtt
    19801 tgcgaactaa acgcagtaat tcctgacgat caacgccatt gagccagaat tgcaggcata
    19861 acgcgtaatt atcatgaatg atttcggtgg tacaacttag tgcaatagtc ggcggcaaac
    19921 taacctgtgc atcaacgaca tcatcttctt caatcgcaac aaatagacgc tcgctcgcct
    19981 caagcgggaa atattttctc atcatcagga tttctctccg gctgcgttat aaagcaagga
    20041 atggcgctga aaaaatgcct ttaaggttgg aattaacgag agcgccacgt ccagacattg
    20101 cccaccgaag taagcaaacg ctgctcccag cacaccaaac agatgacaga acatgaacag
    20161 caatacgatg tagccgattg aggcgatggt ttgcgccacg agaaaagcgc gctgttttcc
    20221 cgccatcaat agcaatgact cctgcgggaa gcccagcatc gaaatcacaa ttgcgcccag
    20281 catcacctga atcaggtcat acgcttcgag atacttaacg ccaaacacca gcgaaatgag
    20341 cggcttgccg acgatcaaca ccgcgagcgc caccagaata ccgattccgc ccgccagtaa
    20401 accggacttc acacctaaca accacggtct ggtggtgcgc ggatctaaac gcatcacctc
    20461 tgggtaaaaa cttttaccca gcaaacccgc tggcgttccg gcggcgtcga agaatgtcat
    20521 ggcgatttta aataacccgg cggcagcggg tcctaacacg atccccacca acactgtgct
    20581 gcacgagtta cgcgccgacc agatggagtg ggcaatgttg gttgaccaga caaaactcca
    20641 cgcgccttta atgtgtcggg cagactcaaa cagattcaat ttgaaggcgt tatggatatt
    20701 tctacggcgt aattcgcgcg cggcaaacca ccagtacatg gtgccgccaa ccagattcga
    20761 cacgtaccag gcaataacaa aacccacaaa accaaagtca aaataccagg ctgcgacgct
    20821 ccccgctgcg cgcagaaaag gtttcgtcgc ctgctgtaca gcaatcaaat caaagcgatc
    20881 taccgcacgc agaatgccgg tcggcgtgga ggaagccatt gaaggaatga gcgtgcaata
    20941 cagcgccgcc agccaaaagc tttggtcatc cagacctaat gaatgggaaa ggaatggcag
    21001 taaggcaatg ccgccgacaa tcgccaccgc gccgctgacg atatccagcg agaaggaaaa
    21061 tgagacgaca ttgcgaaatt gctgcggatt attgttggtc aaggctggcg ttccgtactg
    21121 aaccaccagc tgccatgtct gaaacttaat aaaatcgctg atcgacttgg cgtacgattg
    21181 cacaatcacc agtacaccaa acatggcggg cgtcatccct ttaccggcac acgagagcgc
    21241 caacagcccc agcaaggcgc tctcgacatt actggagcct aaccaggcgc tattgcgaat
    21301 aatggtgcga aacgcgccat ctgcaaacca atgtttgatg ttaaaacccg ccaattcagc
    21361 ctgaccttat cgttgatagt aaaaaatatc ccgccagcct taagttaaac ttcggcggtc
    21421 agaaacgatg gcaaccagag aaaccgcctt ctgtacctgt tccagcactt cgctgtaggg
    21481 cgctctggaa tcaatctcaa gaatttttgt gccgttatag ccaatcgtcg acatgacgcc
    21541 gattttgtcc tgcagctcgg catactcatg gtcaggcttg cgggagatgg cagtatcaat
    21601 atcaatcccc aaacgaatga ttaattcagg acggtattgc gccatttttt ggtataaact
    21661 ccgttcgcgc tgcgccagaa acatgctgat tttcccggtc gcacgttcga cgccgatccc
    21721 cggtccgtca taataaaagc ccgaaatttc agcctgcggg aagcgatcgc tgaccaccag
    21781 aacgccactt tgcgccagtc gctgaacctt gcgtagattc gccattcttc gcagcgagaa
    21841 gcagtacata atcaccgccg cccatagcgc cggagatttg gttttcatgc tttgggtttt
    21901 cgaggatttg gccgccaatc gccgttccag ccagacgcca accaacggca atcgtttgat
    21961 tttgtcgccg tcttcgccgg agagcagccc cagatagcgg cgctcggttt gccagtgttg
    22021 ttgcagcgat ttcaccaggt cggtggtcag cgtggattta ccggtgccat cacacccgac
    22081 caccgcaatc agccccggaa tgtagttggg ctgcggcgcg gttgtactat taacagtttg
    22141 tgtttgtagt gcatccattc gttaaatcat cccctatcca ttttggtgat caggaaagcg
    22201 cggcgtgaat tgcgcgtagt gacgcttgca taatttcatc ggctggttgc cgaccatcca
    22261 gttcaaggat cttggcgcca ttgaatgtca gttgcggcgt aacggcgatt ttttcctgta
    22321 acgctgccag ttggtggtca ggtttacgcg caaacgcggt ttgttcatca atgccaagac
    22381 gaatcaacaa tacgggtaaa taagacgcca tccattggta cagcttcaac tcgcgctgcc
    22441 ttaacatttt tatccaaccg ttaccgcccg tggtttttgc caattgcggg ccatcaaagc
    22501 gaaaacccgg cacttcaacc tgcgggtagc ggtcggtgat gagcagatag ccttgctggc
    22561 ttttacacaa cattttgcga aacttgtacg cccgccagca ggaaagcaga tagataacca
    22621 gcgcagtaat attgccaggc ggagttaagg gcttttcgtg tacatgtgcc gctttacttc
    22681 gcagataaca cccacaaggt gcgccaataa cagggagctg tgaaatccat tcgccaattc
    22741 gcccggacga ttgcccgaga taaatgtgtt ctgttggcat tcttgctgcc agttcattta
    22801 ccaggcttgc cgtgagggtc gatttacctg aaccatcaca tcctacaatg gcaataacac
    22861 gcactggcgt ggaattaatt attgacatat tacgttgatt cacgaaaaac ccggcagtaa
    22921 ataatgtatt gaatattacg tggtccgtgc tcagaatatc cgttcaacct tattgtgaca
    22981 atgactaatt gtgacattga cctgaattac gctttcataa aaacatatta accaaataaa
    23041 tatttttaat ggatatttaa attaaaggat atattcatgc agtcaataac acctccatta
    23101 attgccgtta ttggtagcga tgcttcaggc aagtcaacgg tgtgtgaaca tcttattacc
    23161 gttgtcgaaa aatatggtgc tgccgaaaga gttcatttag gaaaacaggc cggaaatgtc
    23221 ggtcgtgcag tgacaaaatt acctttgatg ggaaaatcct tacataaaac aattgaacga
    23281 aatcaggtga aaacagcaaa aaaattgcct ggaccagttc cggcgctggt aattacagcg
    23341 tttgtcgccc gtcgcttact gcgctttcgt catatgcttg cctgtcgtcg tcgcgggtta
    23401 attgttctaa ccgaccgtta tcctcaggac caaattcctg gcgcttacga tggtacggtg
    23461 ttcccaccta acgttgaagg tggtcgtttt gtctcatggc tggcaagcca ggaacgtaaa
    23521 gcgtttcact ggatggcgag ccataagcct gatctggtca tcaaactcaa tgttgacctt
    23581 gaagttgcct gtgcacgtaa acccgaccat aaacgggaat cgctggcgag gaagattgcc
    23641 ataacgccac agttaacctt tggtggtgca caactggttg atatcgatgc caatcagcca
    23701 ctggaacagg tgttggttga tgcagaaaaa gcgattacgg attttatgac cgcgcgtggt
    23761 tatcactagt caaaaatgga aatgcccgat cgccaggacc gggcattttc aggaaggtta
    23821 aatcaactgc aatgctatcc agtacagccc accagaaaga aaaattgccg ccggtaaagt
    23881 aaacacccac gccatcagga tgctggttac cgttttacgc tgtaacccac cgccgtccac
    23941 caccatcgtc cctgctaccg cagacgagag gacgtgtgtt gtggagacag gcatcccaat
    24001 ataactggca aggccgatag acactgccgc cgtcatttgt gccgccatgc cttgcgcata
    24061 cgtcatgccg cgcttaccaa tcttctcacc gatggtcatc gctacacgac gccagccaat
    24121 catggtgcca atgccgagcg ccagtgctac cgccatgatg atccacaccg gagcgtactc
    24181 aatggtgctt aacatatcgc tgcgcagttt tttcagcagg ttctggtctt ctttactgac
    24241 gcctggcagt ttcgccagct tcgcggaggt atcggagatg cacagcataa tgcggcgcag
    24301 ctggctgcgc tgactcacgc ttaacggctc gtaactttcc atattgcctg gcagcatcgt
    24361 tttaacgcgc gcaatcgcat caaaggtatt tgccggatga cagtgaaact ctgttacttg
    24421 cgtgccatca gtcgatgctg caggcaatgg aggttccatc gcaatcaact tctgcggcag
    24481 ttcaggatgc tgttgcaggt agtgttcgaa gttggtaacg gcatcgcggg tacgggtaat
    24541 ttcatagccg gacgcattca tattgacgac gaagccagca ggggcaatcc ccaccagtac
    24601 cagcattacc aggccgatcc ctttctgccc gtcgttcgcg ccgtgcgaaa acgccacgcc
    24661 cgcagcggaa acaatcagcg caatacgcgt ccagaatggc ggtttacgtt tgcccttttt
    24721 ctttttgcga tcttccggaa tgcggtgaat acggtcacgc tttttcgtcc cactccagaa
    24781 acggcgcagc aggaaaatca gaccacccgc aatgaccagg ccgacgatag gggaaacaat
    24841 cagcgaggag aaaattttgg ttacttcacg caggtttaac gcatccatca ccgatgagcc
    24901 ggttaacagc gcgttggtta aaccgatgcc gataatcgca ccaatcaagg tgtgcgaact
    24961 ggaggccggt aaaccaaaga accacgttcc caggttccag ataatcgccg ccagcagcat
    25021 ggaaaagacc atcgccaaac cgtgagttga ccccatattc agcaacaaat cgattggtaa
    25081 catatggaca atggcatagg caacgctaag tccgcccaat aacacgccaa aaaagttaaa
    25141 aaatgccgcc atcaccacag caagttgtgg ctgcatggca cgagtataaa taacggttgc
    25201 caccgcattc gccgtgtcat gaaaaccatt gattgcttcg tagaacaaca caaatgccag
    25261 agcaagtaat aacaaaagcc ccgtatatat atcaaggcca acaaataaat ttagcatata
    25321 ttagattacg ccattttgaa tttacgaacg gacgcattat cagtgacttt aacggcatgg
    25381 gcaaagtgaa atatcatttt ttaacgataa aaagagtgtt atttcttgtg tatcagacaa
    25441 taagcattca ttcaaaacct ttagaaataa tcaaaacaca ggtattccac tggttaatat
    25501 attcacatat gaaatgaata aatattggaa tatataaata ttgaatattt tgattaatcc
    25561 ccgtactaat tattcttcat aatcagtacg gggttgctac aacatgtatt actttttcac
    25621 cacaattaac ggttcaatat cactctcttt tttgatgacc agtgattcat caccgcgcaa
    25681 acacgtccca ccgtagttgc cgccaacggt gaaggtacat acctgaatgt atttaccgtc
    25741 cactttcggc aaacaccaca gttgctgata gatgtttttc tgctcggcaa atttaccgct
    25801 ggttttgtcc agcacctctt catgatggct gaccaggtcg atattgctac cacagcgacc
    25861 ggcgatcggt ttcactgcgt aacctgtttt caccaattca tcattaacag tgaaatcggt
    25921 atccagcagg taacgatggt gcgggaacag cgaccagagg atcggcagaa tcgctttgtt
    25981 gccggggatc accgtccaca gcggctcaaa gaccagcact tccgggcgca gcaatacgtc
    26041 gataagacgc acttcgtttt gcggatgacc ggtacggatt ggcaccgcag caaactcacg
    26101 gtcgctaact tcacgaatct gatcaaacgc ggtttcccac gcccaggttt tccacacgca
    26161 gttaaccagt cgcccttccc catcaatcag ttgcccggca gcatcccagc ccagttcatc
    26221 cagcccgcgc aagatacgcg tttcaaagcc cgcctggtgc agcgcctgct ccataaactg
    26281 cgcgtgatag ttttcctcga tatctttgtc ctgcatgata tggacaaacg gacgtgcacg
    26341 actgtgtttc caggcaccag ccagttcgtt aatcagccct tccgccggat tgaagccgtt
    26401 gcctttatag ccctgctccg cccaacgttc gaggatcaag cccgcttcgg tatgacagga
    26461 ggcggagtcg gcgttgtatt cgtaaacctt caggccacgc tcatccatgc agaaatccat
    26521 acgaccagtg atcatatggt gacggcgacg ctgccaggag agacgcaaac gtggccagag
    26581 gattttcgga atgtcgaaca gcgccagcag gttgtcatct ttcagcacct tgtcggttgc
    26641 gtgaagatac atcagatgca gctcgttggt ggctttaatt agctcctgct cggcactctc
    26701 ggtaatggtg tagtaatgat acggatcctg attgatcacc tgaccgttgg cctgcacata
    26761 ggcgttttgt agcggatctt tttcatccag ccatttaccg tcaaactggc ctttgttttc
    26821 cagacgcgct ccactgattt tcagcagctc gcctgcaatt tccggctgcg gtaagctgta
    26881 ttcagtatct tccgtctgga tcatccagcc cagaatggtg gtgtcatcaa aagtgtcttt
    26941 aagggtatag cagccgtttt cgaccaccat ctccagctcg cgcgtccact gttgcccttg
    27001 cggcaacggg gaatgaatca cgttctgttc cgcaatacgg actttgttgc catgcaattg
    27061 ggtaatgatg gcgacatggc cagtgtcttt aaattcaccg cctttatccc agataagaag
    27121 cgcacccgcg accggcgcac gcggcgagcc gttaggaaat gcctgcaatg gcaggatgtt
    27181 gtcattaacc acttctcgca ggaagcgcag cgagaaaatc tcccacgcca tacccacgtc
    27241 agtaaagacc acaccgtaat tcagaaagag aaaacggcga gcaaattcaa cgcattgcca
    27301 cttgtggccc atatattcgt cgtcgatata gctacggaat acggcgtcat cttcgtattc
    27361 ctgcggatcg agagaactgt aatctgaaga gtagattgct accccacctg gggcgtagcc
    27421 caataatgtc ccgaacgggg catcctggct ggtcgttcct ttgctcatca ctttaccttt
    27481 aacaatacaa cctaagcagt tggcgtaatt gttgtgctct gattacctgc tcagcgacat
    27541 taaccggaca gaggtcagac taatcataca cctcatcgca gcggctggcg caaagggtta
    27601 aaaaattcac attctgtaca gcaagtgacc tgctacactg cttcaacact accactcaga
    27661 aggcaactca ctatgacaga caatacttat cagcccgcga aagtctggac gtgggataaa
    27721 tccgctggcg gcgcgttcgc caatatcaat cgcccgtttt ctggtccgac gcatgaaaaa
    27781 acgctgcccg ttggcaaaca tccattgcaa ctttattcgc tgggaacgcc gaacggtcag
    27841 aaagtaacga ttatgcttga ggagctactg gcgctgggcg ttactggtgc agagtacgac
    27901 gcctggctga ttcgtattgg cgatggcgat caattctcca gcggctttgt cgaagtgaac
    27961 ccaaactcga agatcccggc gctgcgcgat catacgcata atccgccgat ccgcgtgttt
    28021 gaatctggtt cgatcctgct ttatctggcg gagaaatttg gctacttcct gccgcaggat
    28081 ctggcaaaac ggactgaaac gttgaactgg ctgttctggt tacagggtgc cgcgccgttc
    28141 ctcggcggtg gttttggtca cttttaccat tacgcgccgg tgaaaattga gtacgccatc
    28201 aaccgcttta ccatggaagc caagcgcctg ctcgacgtgc tggataagca actggcgcag
    28261 cataagtttg ttgcgggcga tgagtacacc attgcggata tggcgatttg gccgtggttt
    28321 ggcaacgtgg tgctcggcgg cgtgtatgat gccgcagagt ttctggatgc gggaagttat
    28381 aaacatgtgc aacgctgggc gaaagaagtg ggcgaacgtc cggcagtgaa acgtgggcgt
    28441 attgttaacc gcaccaacgg gccgctgaac gagcagttgc atgagcgcca tgacgccagt
    28501 gattttgaga caaatacaga agataagcgt caggggtaag ggttggtgtt cgtcgcagca
    28561 agccatccag gccggataag gcgttcacgc cgcatccggc aatcgtgcac aatgcctgat
    28621 gcgacgctgc cgcgtcttat caggcctaca aaagctaatc atcacgcgct ggtaatgtcg
    28681 taatccattt ggcgcagtgc gtctaatgtg gctttggctt catcttcgtc gatgatgctc
    28741 atggcaaatc cgacgtgtac cagcacccac tggcccagca gatcggcagg gttaccttcg
    28801 caaatcaggg cgatattcac gtcgcgcttg ataccacata cttcaacctg cgcaagctgg
    28861 tgaatatctt caccgacagc cagcacctgg cctggaacgc caatacacat tgttaactcc
    28921 ggttatttcg attgccggac aaacgcgaaa aagaaccttt agcgggtgtt ccctctcacc
    28981 ctaaccctct ccccggaggg gcgaggggac cgtccgtgcg ctttttaggc gttgtcctca
    29041 gtcttattca acttcaatac ttttgacgat cagcgaatcg ccggtatcga cacgcaaccg
    29101 ctcgccgtga cagagcggac actgcgcatc gtgctgatga atctccacca cctggctgca
    29161 atcccagcac caggcctggg cgggtttata gacgatatgt aaatcgcacc cttgcgccac
    29221 cgttccgtgg cagacaattt caaaactaaa acggacggcg ctctcctcaa cgcaggagag
    29281 cgcgccaatt tccagccaca cggcggtgac gcgcttaaca tcgtgctgct ccgcctgccg
    29341 ttggataatt tcaacggcgc tctggcaaag cgacaactca tgcattttcg cccctcctgc
    29401 ggccaaacag cagggcgcga cgccctgcgt gtggtacatc cggattggtg actggcaacg
    29461 aaaggatcat tcgtgcgcag tcatccgtca ggcgttggcc ttcttcaatc gacatgctgt
    29521 gcgaaagcgg cgacatcagc gaacaggaga ggtattgcga aacaccgtcc agttcgccaa
    29581 cggtaaaggt catggtgcca tacggcagtt gcagaccaat tttttcactc actttgcgca
    29641 gcggccagag ttgatccggg ccggggaaga taactgcact cagcatccac ggggtgatca
    29701 cacaccccgt ccactgacct tcgaacagcg taaaatcaga aacatacacc ggcattgaag
    29761 gatgcagaaa agagagatcg tgcatcgaac gccgggcaat ttcttcaaac gctgcctgta
    29821 cttgcgcctt cggggaggtc tggaaacctg ctatctcttc agtcatgaat cgcctcccgt
    29881 gggatggctt ccacgcctga ttcgcgcagc gcagccagaa cctgctcaag cgcaggttca
    29941 atcattgctt caaccgtcgg cgttaagccg atgtgtggct ccagcgattc cgggatcacg
    30001 ccgaccaggg tcagcttttt cggaaactca ccggtgaagc gcagggccga caatacgtcg
    30061 gccaggccaa gctgatgcgg agagattttg ttggtaaaca acgccggaac ttcttcatcc
    30121 cgcaggatca tcatcgttcc cggagtgttc tttttcgaca caatggcatc cgcaataatc
    30181 agatgatcgc gatttgccat gtcgccaagc agctccattc ccgccgtgcc gccatcgagg
    30241 atctcaacat aatccggcag aatgtatcgt tgctctaacg cttcgacaat ccgcacgccg
    30301 atggcttcat cggtcagcaa aatattgccg acccctaaga ctaaaatacg cattacagaa
    30361 ccttcactga aaccacttcg ttgccgtcgg catccactac gtgtaccgca caggccatgc
    30421 acgggtcgaa ggagtgaatg gtacgcacca cttccagcgg tttattcgga tcggcaaccg
    30481 gtgtacccac cagcgactgc tcgtaaggac cgacgtcatc attgaagtta cgcggaccag
    30541 agttccaggt tgatggaaca accgcctggt agttgctgat gataccgtct ttaataacca
    30601 tccagtgaga gagcataccg cgcggcgctt cgaggaagcc aacacctttg aactcacccg
    30661 ttgccggaat gttcggcttc acaaaggtgg tgtgatcgcc tttgccgata ttggtgatca
    30721 gtgcactgta ttggttttgc aggatatcct gcaattcaca gcagtgaacg gtacgaccaa
    30781 taatacggcc cagcgtggag tgcagctgtg ccacttccag cgtgttgcca gtcagtttct
    30841 gataaatcgc aacgatttca ttcagtttgt tctgggtaga ttcgcgacct gccgccagtt
    30901 tcaccagcat attagccagc ggacccactt ctaccgtttt gccgtagaaa gtcggtgatt
    30961 tcacccagga atatttcccg tcgtcagacc aaccatcata agccggaatg gtggtacctt
    31021 cccacggcgc ctgtggcgct tcgtctttat accaggagtg cttcgcgctt tcctgaatgc
    31081 ctttgatcag gtattcatcg gaatgagaag tgatcggacg atacgaggac agatccgcat
    31141 tctcaatgta gcctcccggg aacaggaagc tgccgttttt gctgtcggtc gggaattccg
    31201 gcacgctcag gtagttcacc gcacctttac cgcgcgtcag ccattccggg tagaacgcgg
    31261 cgataaccgc ggtatcaacc ttataaacct gctcaacaaa gtcgctcagt ttgtcgatga
    31321 aagacttgat gtacatcagg cgctcaaggt tcaacacgcc caacccgtcg aggttgattg
    31381 ggttcgcgac accacccaca gccaggttct gaatgtgcgg cgttttaccg cccagcagcg
    31441 ccacgacgcg gttagcgtca cgctggcact ccagcgcctg caggtagtgc gctaccgcaa
    31501 tcaggttcac ttccggcggc agtttcatcg ccgggtgacc ccagtagcca ttggcgaaaa
    31561 tacccaactg accgctggca accagatctt tgatcttgtt ctgaacttta gtgaactctt
    31621 ccggactgtt cagatgccag gtcgaaacgc ctttcagcat ttcggaggct ttggttgggt
    31681 cagcttgcag tgcagaagtg atgtccaccc agtccagcgc cgaaagctga tagaaatgaa
    31741 cgatatggtc atgcgtggtg tgcgcagcca gaatgatgtt acggatgtat tgcgcgttaa
    31801 ccggaacgtc gatattcagc gcactttctg ccgcacgaac ggaagacagc gcgtgagtgg
    31861 tagtacatac gccacagata cgttgcacaa tcatccatgc atcgcgcgga tcgcggtttt
    31921 tcacgatctc ttccatgccg cgccacatgg taccggaagc ccatgctttc gaaacgacgc
    31981 cattttcgat ttcgcaatcg atgcgtaaat gcccctcaat acgggttacc ggatcaatag
    32041 taattctctg gctcatgctt tgctcgcctc atgacgatta tgatcgtttt gttttaaagg
    32101 aggaagtatc ggcagtagac gaatgagtac gatgtaagcg caaatctcaa tagccacaaa
    32161 accaatagaa atcaacagtt cttcccaggt cgggaagtag gcgtaaccgc cgcccgggtt
    32221 gaatgccacc aacgaatagg tcagacgcca ggttgcacaa cctaacagtg cgctcagtgc
    32281 tgacaggaac agcatacggg aatcattacg cagcttcgcc acacgcagaa cgaccagcgg
    32341 gaagagcatc agcaggactt caatccagaa catcacggag tagaagtcac cggcaaacgc
    32401 taacgacagc ttgtcgcgat agataagctc gccaaagcgc agcccgatga aaatcgccag
    32461 caacacactg atggtgttgg tcagcttgac gaacaggctc ttttcatccg gaccgttgcc
    32521 acgcagaccc gcctgcacca gtgaaccttc aaagatgaca atcgagaagc ccatgatgaa
    32581 cgccgtcagc agcgagaaca gcggcaacat ttcatagctc tgccagagcg gatgcacctt
    32641 gtagcccgcc gagatcatca gcgaccccat tgaagactgg tgcatggtcg gcagcagcgc
    32701 accgagcgcg atgatgaaga acatcacctt gttcagacgt tgtagcgaca ccttccagcc
    32761 cagacgttca aacagtgccg gagcaaactc cagagccatc acgccgatgt agatggtcat
    32821 acagaccgcc gtctcgaaca gtaccgagtt cacgttgaag tgacccggaa tgtagaagta
    32881 cggcaggttc cagtagcgac ccacgtcgat agtgatcgac aagccaccca gtgagtaacc
    32941 aaacagactc gccaacagcg ccggacgcac cagcggatgg tattgcccac ggttaaagac
    33001 gtataccgcc cacgccagcg cccagccgcc acaggcaaag ccggtgccaa tcagcaggtc
    33061 aaacgcgatc cacacgcccc acgggaagcc gccgttcagg tcagagacag agcccagacc
    33121 gaacaccaga cgcttcacaa tcaggagcat acagatgacg attaacggtc caaaaatcat
    33181 gaccggttta ctgatgattt tgccgcccag cggttgtgga tcatgactca tgatcgtctc
    33241 ctccgtcgtg atggtcgttt ttggtgttgc gacgaaccag cacggttaag cccgccagca
    33301 cagccagtgg tagcatcatg cctttataca gggtgtgttg aatattttcg gaacgcgcac
    33361 cggtagaaag atcgtccagt ttcggcaggt cgagattttc ataaggcaca cccgtcagta
    33421 ccagaacctg agtaccgccg ccctctttct cgccgtacag atgcggataa tatttcggca
    33481 ccgtatgcag gtaagtgtcg ccagatttca gcgtctgacg cggatagtgg tattcgctgc
    33541 caggcttcag cgccagacgt tttttcgcct ccgccatcag ctcttcacgc gtaccgaaaa
    33601 tcaccgcgcc cgccgggcac acttctacgc agccaggcag accgccttta tcgagacgtt
    33661 ccacaccttt ctggttgcac agctcgcact tatgcagcgc accaaacggg ttgttgtagt
    33721 cgtacttcgg cacgttgtac ggacaggcga ccatgcagta acggcaaccg gtgcacacgt
    33781 ctttgtcgta atggacaatg ccggttttcg gatctttttt cagtgcagag accgggcaca
    33841 cagagacaca gttcggatcg acgcagtgca tacactgttt cttaatgtac gcgtagccgt
    33901 tctcctcctg gtctttgttg acccctgtgc cgctggtcca cacctgaatg atgttattgg
    33961 tatacggcga cagtttgtcg ttgttcgacc aggtctgttc cccttgcggg ttacgttcag
    34021 ggaagttgat atcctgacac ttggtgacgc aagcctggca gcctacgcac aaggtcgagt
    34081 cgtacaacat ccccagcgat cccggaattg gcgggcggtt ttcagcagcc gcatgactga
    34141 cagacggcag cgcgcccgtc agcaatgccc cgcaggaggc tgctttaata aaattacgtc
    34201 tgttcacggt tattctcccc gtgagtcagc gttatctttc ttttgctgac gacccagttc
    34261 acgcaccgcc atcacgctga caccggcaac cagcccaacc acaccgccga gcaaaccaat
    34321 agcgcctgca gagacgttgc cgccctcttt agcgttaaca tccggtttct gtgaacgcgg
    34381 agtctgattt tcgacgttgg caagctgatg gatgccttta tggaagccga tacctttttc
    34441 gttacagcca tagcacgggt gaccaatcgc caccggccac acaccgccaa catcgcagaa
    34501 ttgcagcgtt gagcagttgc cgtaagtttc tggcccttta cagccgaggt ggtacaagca
    34561 ccagccttcg cggtggcctt catcaccgaa ctctttggca aaacgaccag catcgaagtg
    34621 cgggcgacgt tcgcagtgtt cgtgaatcag acggccatag gcgaaggtcg gacggttttt
    34681 gtcatccagt ttcggcggtt tgccgtaagt gatgatgtgc gcaacggtcg cgaggaagtt
    34741 gtgcgggttc ggcgggcagc ccggaatatt gataacggtt ttgcctggca gaacttcttg
    34801 caagctgact gcgccagttg ggttaactcc agctgcggca acaccgcccc acgcggagca
    34861 ggaaccaata gcgataatgg ctgctgagcc ttccgccgct ttgcggatgt gatccacaat
    34921 cggctcaccg gcaaccatgc aataaatacc gttatctttt aatgggatgg aaccatccac
    34981 cactaacaca tactgccctt tgtacttctc gagagcgtta tgtttgttct cttcgacctg
    35041 atgaccgaag gcggcggaaa gcacttcgtg atattccaga gagatagtct ccagcacgag
    35101 gttttctacc gttggatgcg ttgcacgaag cagagattcc gtacaaccgg tgcactcctg
    35161 cgcgccaatc cagataactg gcggacgctg cgggttagta accgattcgg ccatctctgc
    35221 agcggctttg ctacttaacc ccatggtggc ggctaatgct gcacaaagct tcatgaaatc
    35281 acgacggtta atgccgtgag aatggatgag ggtgttatct ccagtcattt atagttattc
    35341 cgttgcgaga acctggcata tattttgcct caatcgcaaa atcaataatg cgatcgatgc
    35401 gccatttacc acacatttat tatggttatc gatatcatga tactgcggcg gggcgctaaa
    35461 acgaagggaa gaagcattag tgtaattaat taaagcaaga taatacgtat gtttgatcaa
    35521 ttttcgaggc gataaccggc cataaaaaag cgcaacgtag aacaggaatt attctcacga
    35581 aagaaatcca ttaccatctc tttatccagt ccatagcggc gcgtcagaat acccgcttcc
    35641 caggcgctaa gctggcgatc tccggtcttt tcgtacatcc tgtgtgagta gcctaataca
    35701 aaaccgcgtt tataatcggc acagaatttt gtcacattaa ccgcactgtc ggcatgcgtt
    35761 gcttttaatc ccgccattaa gcctttacca aaatggttat tcatcgttca cctcattcat
    35821 tatatatcgc catatataac gatattttag gcaaagaacg acttttgcgc acgctatttt
    35881 ttcatacttg tttaagacgt aatcccgtaa gagataaacc tatcaggaga gatatttatc
    35941 gaaccatttc agtgtccttt gccaggcaag atcggcggca gatttgtcat aacggggcgt
    36001 ggaatcatta tggaatccgt gattaacccc cggatagata taagcctcat aaaccttatt
    36061 attgtctttc aacgccgcct cgtaaggagg ccagccctcg ttgattcggg tgtccagttc
    36121 cgcgaagtgg agtagtaaag gcgcttcaat cttcgccaca tcggcagtgg gtgcctgacg
    36181 accataaaac ggcaccgcgc aggccagttc cggatacgcg acagccgccg cgttcgatac
    36241 gccaccgcca tagcaaaatc cggtaatacc cactttacct gtcgcttgcg gatagcgttg
    36301 cataaactca attgcggcaa agaaatcatt catcagtttg gttggatcaa cctgctgttg
    36361 cagctcacga cctttatcat catttcccgg ataacctcca acggaactta atccgtcagg
    36421 tgccagggcg atataccccg ccttcgccac tcgccgtgcc acatcttcga tatacggatt
    36481 cagtccacga ttctcatgca ccacccccac ggctggcgtt ttgccgctca tttttgcggg
    36541 tttcaccaga taaccccgta cctcgccgtg accatttggc gaaggatacg tgatgtactc
    36601 agcaacaatc tccgggtcgg taaactctac ctgagtcgcc agcgcataat ttggcttgag
    36661 caaatcaaac aacgctaatg ccgtcatccc gcccaccgca tacttcgccg caaggttgag
    36721 gaactcacgt ttcgaaattt tcccgtgaac gtaatagtcg tagtaatcca acaactcttg
    36781 tgggaaatct ttggcggtta aacgcggcat cgttgcattc ctcaactggt gtttttttaa
    36841 gcaaagcata agcgcgtgtt tttgcccagt ttttcgtcac tctgtgagcc agactacggg
    36901 atacgcgctg gcgaatcgct aaactagaaa cattgtttcg aaattgaacg gtggaaagga
    36961 gaggtcatgg tctggttagc gaatcccgaa cgttacgggc agatgcagta ccgctattgc
    37021 ggaaaaagtg gtttacgcct gcccgcatta tcgctcggtt tatggcacaa tttcggtcac
    37081 gttaacgcgc tggaatcaca gcgtgcaatc ctgcgtaaag cgtttgattt aggcattacg
    37141 cactttgatt tagccaacaa ttacgggccg cctccaggaa gcgcagaaga gaactttggt
    37201 cgcctgctgc gcgaggattt tgctgcttat cgcgatgaac tgattatctc taccaaggct
    37261 ggctacgata tgtggcccgg cccgtacggc tctggcggtt cacgtaaata cctgctcgcc
    37321 agcctcgacc aaagcctgaa gcgtatgggg cttgagtatg tcgatatctt ttactctcat
    37381 cgcgtcgatg aaaatacgcc gatggaagaa accgcctctg cgctggctca tgcggtacaa
    37441 agcggtaagg cgctgtatgt cgggatctcc tcttactcgc cagagcggac gcaaaaaatg
    37501 gtcgagttgc tgcacgagtg gaaaattccg cttttaattc atcaaccttc gtacaattta
    37561 ctgaaccgct gggtggataa aagcggcctg ctggataccc tgcaaaataa cggcgtgggc
    37621 tgtattgcct ttactcctct ggctcaggga ttgctgaccg gaaaatatct caacggcatt
    37681 ccgcaagatt cacggatgca tcgtgaaggg aataaagttc gtggtctgac gccgaaaatg
    37741 cttaccgaag ccaacctcaa cagcctgcgc ttattgaatg aaatggcaca gcagcgtgga
    37801 caatcaatgg cgcaaatggc gttaagctgg ttgctgaaag atgatcgcgt gacgtcggta
    37861 ttgattggtg ccagccgcgc ggagcaacta gaggagaacg tgcaggcgct gaataatctg
    37921 acatttagca ccgaggagct ggcgcagatt gatcagcata tcgccgatgg cgagctgaat
    37981 ctgtggcagg cgtcgtccga taaatgacct gttaataacg ggcgcgcgaa ccgcgcccga
    38041 atcagatcag tgattatgac gagtcagtcg gtcaagatag cccatcacaa atgcagaaag
    38101 cacaaacgtc agatggataa tgacgtacca catcagtttg ttatccggga catttttcgc
    38161 atccataaag acgcgcagta agtgaatgga agaaattgcc acaatcgacg ctgctacttt
    38221 gtttttcagc gacgttgcgt ccattttccc cagccacttc agcttctctt tgttctcgga
    38281 gatatccagc tgtgagacga aattctcata accggaaaac atcaccatca ccagcaaacc
    38341 gccaaccagc gtcatatcca ccagcgacag caacacgagg atcaaatctg attccgccat
    38401 cgagaagata ttcggcagta cgtgaataat ctcctggaag aacttcagcg ccagggcaac
    38461 taacgccagc gaaaggccaa agtacacggg ggcaagcagc cagcgagaag catacattgc
    38521 attttcaaga aaacgttcca tacattcctg tcgtgtagtt aaaacgggca acagtatatc
    38581 gcaattgagt aaacagcacg caacagcaaa tcaacatata cgttaaacct ttgtcgcata
    38641 attctgaggt ttagggatct tcctggttcc cttatcaacg catcgagagc gtctaccctt
    38701 aaaggacgtt gaacctgaag ggagaaaaac gatgtctcat ttaaaagacc cgaccacgca
    38761 gtattacact ggtgaatatc ccaaacagaa acaaccgacg ccaggcatcc aggcgaagat
    38821 gacaccgata ccggattgcg gcgagaaaac ctatgttggt agcggtcgcc tgaaagatcg
    38881 taaagcactg gtgacagggg gcgattccgg aataggtcgc gctgccgcca tcgcttacgc
    38941 gcgtgaaggg gctgacgtgg cgatcagtta tcttcccgtg gaagaagaag acgctcagga
    39001 tgtgaaaaag atcattgaag aatgcggacg caaagccgtt ctgctgccag gcgatttaag
    39061 cgatgagaaa ttcgcccgtt cgctggttca cgaagcgcac aaggcgttag gcgggctgga
    39121 tattatggcg ctggtcgccg ggaaacaggt tgccattccg gatattgcag acctcaccag
    39181 cgaacagttt caaaagacct ttgccattaa cgttttcgcg ctgttctggc taacccagga
    39241 agcgatcccc ctgctaccga aaggtgcaag tgtcatcacc acttcgtcaa tccaggcata
    39301 ccagccaagt ccgcatttac tggactatgc ggctacgaag gcggcgattc tgaactacag
    39361 ccgtggcctg gcaaaacagg tcgcggagaa aggtattcgg gtgaatattg tcgcgccagg
    39421 ccctatctgg acggcactgc aaatttccgg cggacaaacg caggataaga tcccgcagtt
    39481 tggtcagaaa acgccgatga agcgtgcggg gcaaccggcg gaactggccc ctgtatatgt
    39541 ttatctggca agtcaggagt cgagctacgt caccgcagaa gtgcacggcg tgtgcggcgg
    39601 cgagcattta ggttaaaaaa atgcccggtt gtgaaaagca accgggcatc attgtgaatt
    39661 cactcttacc tgatgtggcg aaaacgcctt atccgcctac aaacgcatga agattcaaca
    39721 tgttgcaata gattttgtag gcctgataag cgtagcgcat caggcattga gcacctaatg
    39781 ccggatgcgt cgtgaacgcc ttatccggcc tacaaacgca cgaagattca acatgttgca
    39841 atagattttg taggcctgat gagcgtagcg catcaggcaa ttctacttta cttcgctttg
    39901 gcggtttctt cgccgaccag acctatcttc aggtaacccg cctgatgcag cgtatccatt
    39961 accttcatca acgtctcgta atcgacggtt ttgtccgctc ggaagaagat ggtggtgtct
    40021 ttcttgcctt cggttaacgc attcaacgcc gtaatcattg tttcatcggt gaccggatcg
    40081 ttaccgataa acatcgagtt gtctgccttc accgacagat aaaccggttt ttccgggcgc
    40141 ggctgcggcg tgctggtaga agcaggcaag ttcaccttca catctaccgt cgctaacggt
    40201 gccgccacca taaagataat cagcagaacc aacatcacgt cgataaacgg cgtcacgttg
    40261 atatcatgca tttcgccgtt atcgtcgagg ttttcgttaa gatgcattgc catcggacat
    40321 tatcctgcgc gtaatttttg tgcgacacga accggatgcg cagcggcgct ggcttccaga
    40381 tctaggtcac ggctttgcag caacaatacc tgcgctgcaa catcacccag catcgcttta
    40441 aagccgccaa tctggcgtgc aaatacgtta tagataacga ccgcaggaat cgctgccacg
    40501 aggccgattg ccgttgctaa cagagcttct gcgatacccg gcgcaacgac tgccaggtta
    40561 gtggtctgcg tttgcgcgat accaataaag ctgttcatga tgccccagac cgtaccaaac
    40621 agaccaacga acggagaaat cgcgccgatg gttgccagat agccgttacc gcggcccatt
    40681 tgacgaccca ctgcggcgac ccgacgctcc agacggaagc tggtacgttc tttaatacct
    40741 tcgttatcgt cgctgccttc tgacagttcc agctcattct gcgcttcatt gagcaaatgc
    40801 aggcttaagc ttttgctacc aaaatcagcg gcgatatcgt tggcctggtt taaggaacgt
    40861 gcttcagcca gcagttgctg ctcgcgctta aggcgacgct tctgattgaa gaactctacg
    40921 ctcttactga agaagattgc ccaggtgact acagaggcca aaataagccc aatcatcacg
    40981 cacttaacga caatatcggc gtactgatac ataccccaga cggaaaggtc cgtctgcatt
    41041 aaattattac ccactgtgta tctccaggac gcaagtcaca aaatctgcgc ataataatat
    41101 caaaacgacg tcgaattgat agtcgttctc attactattt gcatactgcc gtacctttgc
    41161 tttcttttcc ttgcgtttac gcagtaaaaa agtcaccagc acgccatttg cgaaaatttt
    41221 ctgctttatg ccaattcttc aggatgcgcc cgcgaatatt catgctagtt tagacatcca
    41281 gacgtataaa aacaggaatc ccgacatggc ggacaaaaag cttgatactc aactggtgaa
    41341 tgcaggacgc agcaaaaaat acactcttgg cgcggtaaat agcgtgattc agcgcgcttc
    41401 ttcgctggta tttgagagta tggaagccaa aaagcacgcg acgcgcaatc gcgccaatgg
    41461 cgagttgttc tatggacggc gcggaacgtt aacccatttt tccttacaac aagcgatgtg
    41521 tgaactggaa ggtggcgcag gctgcgcgct atttccctgc ggggcggcag cggttgctaa
    41581 ttcgattctt gcttttgtcg aacagggcga tcatgtgttg atgaccaaca ccgcctatga
    41641 accgagtcag gatttctgta gcaaaatcct cagcaaactg ggcgtaacga catcgtggtt
    41701 tgatccgctg attggtgccg atatcgttaa gcatctgcag ccaaacacta aaatcgtgtt
    41761 tctggaatcg ccgggttcca tcactatgga agtccacgac gttccggcga ttgttgccgc
    41821 cgtgcgcagt gtggtgccgg atgccatcat tatgatcgac aacacctggg cagccggtgt
    41881 gctgtttaag gcgctggatt ttggcatcga tgtttctatt caggccgcca ccaaatatct
    41941 ggttgggcat tcagatgcga tgattggcac tgccgtgtgc aatgcccgtt gctgggagca
    42001 gctacgggaa aatgcctatc tgatgggcca gatggtcgat gccgataccg cctatataac
    42061 cagccgtggc ctgcgcacat taggtgtgcg tttgcgtcaa catcatgaaa gcagtctgaa
    42121 agtggccgaa tggctggcag aacatccgca agttgcgcga gttaaccacc ctgccctgcc
    42181 tggcagtaaa ggacacgaat tctggaaacg agactttaca ggcagcagcg ggctattttc
    42241 ctttgtgctt aagaaaaaac tcaatgatga agagctggcg aactatctgg ataacttcag
    42301 tttattcagc atggcctact cgtggggcgg gtatgaatcg ttgatcctgg caaatcaacc
    42361 agaacatatc gccgccattc gcccacaagg cgagatcgat tttagcggga ccttgattcg
    42421 cctgcatatt ggtctggaag atgtcgacga tctgattgcc gatctggacg ccggttttgc
    42481 acggattgta taacattgcc acttttggac aattttgcag acattttatt gtgaaaagtc
    42541 ttaaattgtt gcgtccggga tcaaggcgtc ccggacgatt caggagtaca ataggcagat
    42601 aaaggcttaa acgctgttcc acaggaaagt ccatggctgt tattcaagat atcatcgctg
    42661 cgctctggca acacgacttt gccgcgctgg cggatcctca tattgttagc gttgtttact
    42721 ttgtcatgtt tgccacgctg tttttagaaa acggcctgct gcccgcctca tttttgccag
    42781 gcgacagctt gttgatactg gcaggcgcat tgattgccca aggggttatg gattttctgc
    42841 ctacgattgc gattctgacc gccgcagcaa gtctgggctg ctggctaagt tatattcagg
    42901 ggagctggtt agggaatacc aaaacggtga aaggctggct ggcacagctt cctgctaaat
    42961 atcaccagcg cgccacctgc atgtttgacc gccacggtct gctggcgctg ctggctggac
    43021 gttttcttgc atttgtccgt acgctgctgc caaccatggc gggaatttcc ggtctgccaa
    43081 accgccgctt ccagtttttc aactggttaa gtggattgct gtgggtcagc gtggtaacca
    43141 gttttggcta tgccttaagt atgattccgt tcgttaaacg ccatgaagat caggtaatga
    43201 cgttcctgat gatcctgcca attgccttgt taaccgctgg cttgttaggc acgctgtttg
    43261 tggtgattaa aaaaaaatac tgtaacgcct gacgattttc cccgttcccg gctgctgtac
    43321 cgggaacgta tttaattccc ctgcatcgcc cgcattcttg ccgcatcttc ccccggcgtc
    43381 acaccgaagt aacgtttaaa ctcacggcta aattgcgatg cgctttcata gccgacgcgc
    43441 atcgctgctg cgctggcctt catgccgtca tggatgatca tcatccgcgc cttatgcaga
    43501 cggtaattct tcaaatactg caacggcgag gtgctggtga cagacttaaa attatggtgg
    43561 aacgccgata cgctcatgtt ggcttctgcc gccagttgct cgacgctcag gttttcggtg
    43621 tatttattct caattcgttt cagcacgcgg ctaatcagac tgaagtgagt ctggcgactg
    43681 accagcgcca gtaacgcgcc gccgcaaggt ccggtcagca cgtagtacag aatttcgcgg
    43741 atgatctgtt tgccgagaat acgcgcatcc agtggtcgct ccatcacgtc gagtaaccgc
    43801 tccgccgcgc ataaaatctc ttctgataac gtggcggagt taatcccgct ggctgccatc
    43861 gacggctgga aatgctcatc ttcgccaatg tccatcaaca gttcctgtaa ctgcaaaata
    43921 tcgacattga gacgcaaccc tgccagcggc acctctgacg tggcataggt ttcgcactca
    43981 aacggcaacg gcaccgtcag cagcaggtat tcattggcat cataacgaaa cacgcgttca
    44041 ttgatataac cgattttatg cccggaaaag agaattatga tgccaggctc gtacatcacc
    44101 ggtgtacgtg cgaaaggcgt ctcgccatac aacaaacgca catcgggcaa cagttctgac
    44161 aaactatttt ctttattttt cagtttatta actttatccg ccagcaagcg acaaatctct
    44221 tcacgtttca tatcgcgtaa tttcttagga ataatgcggc aatttgattg tgcgcaattt
    44281 tgtagcattt ctccagcact ctggagaaat aggcaagaca ttggcagaaa tgagcattga
    44341 gagccagggc gctggcgatc acaatgaaaa acatcaggca gatcgttctc tgccctcata
    44401 ttggcccagc aaagggagca agtaatgaac aactttaatc tgcacacccc aacccgcatt
    44461 ctgtttggta aaggcgcaat cgctggttta cgcgaacaaa ttcctcacga tgctcgcgta
    44521 ttgattacct acggtggcgg cagcgtgaaa aaaaccggcg ttctcgatca agttctggat
    44581 gccctgaaag gcatggacgt gctggaattt ggcggtattg agccaaaccc ggcttatgaa
    44641 acgctgatga acgccgtgaa actggttcgc gaacagaaag tgactttcct gctggcggtt
    44701 ggcggcggtt ctgtactgga cggcaccaaa tttatcgccg cagcggctaa ctatccggaa
    44761 aatatcgatc cgtggcacat tctgcaaacg ggcggtaaag agattaaaag cgccatcccg
    44821 atgggctgtg tgctgacgct gccagcaacc ggttcagaat ccaacgcagg cgcggtgatc
    44881 tcccgtaaaa ccacaggcga caagcaggcg ttccattctg cacatgttca gccggtattt
    44941 gccgtgctcg atccggttta tacctacacc ctgccgccgc gtcaggtggc taacggcgta
    45001 gtggacgcct ttgtacacac cgtggaacag tatgttacca aaccggttga tgccaaaatt
    45061 caggaccgtt tcgcagaagg cattttgctg acgctaatcg aagatggtcc gaaagccctg
    45121 aaagagccag aaaactacga tgtgcgcgcc aacgtcatgt gggcggcgac tcaggcgctg
    45181 aacggtttga ttggcactgg cgtaccgcag gactgggcaa cgcatatgct gggccacgaa
    45241 ctgactgcga tgcacggtct ggatcacgcg caaacactgg ctatcgtcct gcctgcactg
    45301 tggaatgaaa aacgcgagac caagcgcgct aagctgctgc aatatgctga acgcgtctgg
    45361 aacatcactg aaggttccga cgatgagcgt attgacgccg cgattgccgc aacccgcaat
    45421 ttctttgagc aattaggcgt gccgacccac ctctccgact acggtctgga cggcagctcc
    45481 atcccggctt tgctgaaaaa acaggaagag cacggcatga cccaactggg cgaaaatcat
    45541 gacattacgt tggatgtcag ccgccgtata tacgaagccg cccgctaagc tttttacgcc
    45601 tcaaactttc gttttcgggc atttcgtcca gacttaagtt cacaacacct caccggagcc
    45661 tgctccggtg agttcatata aaggaggaac gtatggctaa tccaaccgtt attaagctac
    45721 aggatggcaa tgtcatgccc cagctgggac tgggcgtctg gcaagcaagt aatgaggaag
    45781 taatcaccgc cattcaaaaa gcgttagaag tgggttatcg ctcgattgat accgccgcgg
    45841 cctacaagaa cgaagaaggt gtcggcaaag ccctgaaaaa tgcctcagtc aacagagaag
    45901 aactgttcat caccactaag ctgtggaacg acgaccacaa gcgcccccgc gaagccctgc
    45961 tcgacagcct gaaaaaactc cagcttgatt atatcgacct ctacttaatg cactggcccg
    46021 ttcccgctat cgaccattat gtcgaagcat ggaaaggcat gatcgaattg caaaaagagg
    46081 gattaatcaa aagcatcggc gtgtgcaact tccagatcca tcacctgcaa cgcctgattg
    46141 atgaaactgg cgtgacgcct gtgataaacc agatcgaact tcatccgctg atgcaacaac
    46201 gccagctaca cgcttggaac gcgacacaca aaatccagac cgaatcctgg agcccattag
    46261 cgcaaggagg gaaaggcgtt ttcgatcaga aagtcattcg cgatctggca gataaatacg
    46321 gcaaaacccc ggcgcagatt gttatccgct ggcatctgga tagcggcctg gtggtgatcc
    46381 cgaaatcggt cacaccttca cgtattgccg aaaactttga tgtctgggat ttccgtctcg
    46441 acaaagacga actcggcaaa attgcaaaac tcgatcaggg caagcgtctc ggtcccgatc
    46501 ctgaccagtt cggcggctaa cgtgcaaatt ctcccggtgg cggtaatgtt ccgctaccgg
    46561 acttttcaga aatcatttat tcccctcgcg tcccgcccgt tgttactctt ccttgttcag
    46621 gaatgccaaa tataaggaca tcatcatgca gagccggaag ctcttaaaag aacaactcat
    46681 ctatatccgg gataaacgca acggagaggt gaaaaacaga tgaaaataat acttctgttt
    46741 ttagcagccc tggcaagttt taccgtacac gcgcagcctc cttcacagac cgtagaacaa
    46801 acagtccggc agatttatca gaactataaa tcagatgcca gtacgcctta tttcggtgaa
    46861 accggagagc gggcgataac ttcagcgcgt attcaacagg cgcttaccct gaacgacaat
    46921 cttacactac cgggcaatat tggctggctg gattatgatc cggtttgtga ttgtcaggat
    46981 tttggcgatc tggtgttaga aagcgtagcg ataacccaaa ctgacgtcga tcatgccgat
    47041 gccgttgtgc gctttcgtat ctttaaagat gataaagaaa agaccacaca gacactgaaa
    47101 atggtggcgg aaaatggtcg ttgggtcatt gatgatattg tcagcaatca tggcagcgtc
    47161 ttacaagcag ttaatagcga gaatgaaaaa acgctggccg ctttagcttc gttgcagaaa
    47221 gaacagccag aatcctttgt tgctgaactc tttgaacata ttgctgatta tagctggccg
    47281 tggacgtggg tggtttccga ctcttaccgc caggcggtta atgccttcta taaaaccacc
    47341 ttcaagacgg ccaataatcc cgatgaagat atgcaaatag aacggcaatt tatttacgac
    47401 aatccgatct gttttggcga agagtcgcta ttttcacgcg ttgatgaaat tcgagtcctg
    47461 gagaaaaccg ccgattccgc ccgcattcat gttcgtttta cgctgaccaa tggcaacaat
    47521 gaagagcaag agctggtttt acagcggcgc gaaggtaagt gggaaatcgc tgattttatc
    47581 cgcccgaaca gcggcagcct acttaagcag attgaggcaa aaaccgccgc cagattaaag
    47641 caatgagctg aattaaataa caattagccg gaacaataaa taaaagggaa cactatatga
    47701 aaacgatttt caccgtggga gctgttgttc tggcaacctg cttgctcagt ggctgcgtca
    47761 atgagcaaaa ggtcaatcaa ctggcgagca atgtacaaac attaaatgcc aaaatcgccc
    47821 ggcttgagca ggatatgaaa gcactacgcc cacaaatcta tgctgccaaa tccgaagcta
    47881 acagagccaa tacgcgtctt gatgcacagg actattttga ttgcctgcgc tgcttgcgta
    47941 tgtacgcaga atgataaaaa aatccccggc agcatgtcag ttgccgggga ttttttttaa
    48001 cgtccaaccg ccgctttagg gcgtttcttc gcaccagcat tcaccggacg agattgcgta
    48061 gacgacgctt tttttgccgt agcaggcgtc tgacgctgag tcgccatcgg cgtatgttta
    48121 gtcaacgccg gacgggtatt gcggttctgg cgacgagctt cacgcatctc ttcaatggtt
    48181 ggcgcaggca ctaagcaatc gcgacggctg ccaatcagat gctttttgcc catcgcttcc
    48241 agcgcctggc ggattaacgg ccagtttgcc ggatcgtggt aacgcaacaa cgctttatgc
    48301 aaacgacgct gtttgtcgcc cttcggtacg aagacgcctt cgctcttata accaatcttc
    48361 gccagcggtt ttttcccggt gtaatacatg gtggttgagt tcgccagcgg cgatgggtag
    48421 aagttctgta cctggtcgag acggaaacga tgctttttca gccacagcgc caaattcacc
    48481 atatcttcat cacgcgtacc ggggtgcgca gagatgaaat acgggatcag atactgctct
    48541 ttacctgcct gtttcgagta ggtatcgaac agctctttaa agcggtcata gctgcccatg
    48601 cccggcttca tcatcttcga caacggccct tcttcggtat gttccggagc aatcttcaga
    48661 taaccgccga cgtgatgggt cgccaactct ttgatatagc gcggatcttc cacggcgatg
    48721 tcataacgta cgcctgaggc gataaggatt tttttaatgc ctttcagatc acgcgcacgg
    48781 cgatagaggt tgatcgtcgg ttcgtggtta gtgtccatgt gcggacaaat atccggataa
    48841 acgcacgaca aacggcgaca agtttgttca gcgcgtggcg atttgcagcg caacatatac
    48901 atgttggcag ttggcccacc gagatcggaa atcacgcccg taaaacctgg aacggtgtcg
    48961 cggatcgctt cgatctcatt aatgatcgaa tcttccgaac ggctctgaat aatgcgccct
    49021 tcgtgctcgg taatagaaca gaaagaacag ccgccaaagc agccgcgcat aatgttgacc
    49081 gaaaaacgga tcatttcgta agccggaata cgggcattgc cataggccgg atgtggcacg
    49141 cgcttgtacg gcagcgcaaa aacgctgtcc atctcttcgg tagaaagcgg gatagcaggc
    49201 gggttgatcc acacatagcg gtcaccgtgt ttttgcatca atgcgcgggc gcagcctggg
    49261 ttggtttcgt ggtgcagaat gcgcgaagca tgggcgtaca gcactttatc gcccttcact
    49321 ttctcgaaag aaggcagcaa tacgtaggtt ttttcccacg gtttctggcg cggtggctgc
    49381 acgattacag ctttcgcttc ctgctttttc ggcgcaacag gtttgttatc cgcacacggc
    49441 aaatcttcgc catacggatg cgggattggg tcgattttcc ctagggtatc aagacgggtg
    49501 gaatccacac cgctccagcc aggcagcgcc tcttttacga taattgcggt attacgcaca
    49561 tcgcggattt cactaatcgg ctcgcccatc gccagacgat gcgctacttc caccagcgga
    49621 cgttcaccgt taccaaacat cagcatgtcg gctttcgaat ctaccagaac ggaacggcgc
    49681 acggtatcgg accagtaatc ataatgcgcg gtacggcgca ggctagcctc aataccgccg
    49741 aggatcaccg gcacatcttt ccacgcctct ttacaacgct gggtataaac cagcgtggcg
    49801 cgatccggtc gcttacccgc gacattatct ggcgtgtagg catcatcatg acgtaaacgg
    49861 cgatcggcgg tatagcggtt gatcatcgaa tccatgttgc cagcagtaac accaaaaaac
    49921 agattcggtt tacccagacg cataaagtcg tctttgctgc tccagtccgg ctgggcgatg
    49981 atcccgacgc gaaagccctg cgcttccagc atacgaccgc aaatcgccat cccgaagctt
    50041 gggtgatcga catacgcgtc gccagtaacc aaaatgatgt cgcagctatc ccagccaagt
    50101 tgatccatct cttcacgaga catcggcaaa aacggtgccg gtccaaaaca ggcggcccag
    50161 tactgcggcc aggagaacag gtcgcgatcc ggttggatca gggagataga gctcattttg
    50221 cttccagaaa tgataaaaaa ataatcaaag gccggggatt ataagccgga acgaaagaga
    50281 aatcgaaagg tattccatac tcgccctcct cgggcgagta tgaagattac ggtaccggat
    50341 tgaccaacag ttgcccaatc gagccacggt ccgccatttc cagcgtctga ctgttgaagt
    50401 agaacgggaa gtgcgcccag gaaggctgac cgaaataaac aagcagctcc acttgtccgt
    50461 caacccaaac ggtatctttc cagcctctgt cttccgggaa cggcatcgcg ccattcacgt
    50521 tacggatctg gaacattacg ccttcaatat ggaacgcttg cggctcgtcc gcgcgtaccg
    50581 tccagcgttc ccacgttcct tgctgcgcgg tgacatcaat acggttgacg tcccacagct
    50641 gtccattaat acccgggtca tcacccagac tgatatcgcg actgcgaatt ggcgaaccag
    50701 ccatgatttc agttggcagc aagcgcatcg gaagactgtc tgtgaccagc ggcagaaggc
    50761 cggttgggcg cagcgttagc accaaggtag aaaccagaat actggatggc tcaaagaagc
    50821 cgcgaatacg atcaacaatg ctcgccgctt caccacaggt gatcgacact tcatcgccgt
    50881 tgctcatatc caccagaatc tcgcggcgct cgcccggtgc cagcgaaagt tgcttcaccg
    50941 atacaggagc aggcaggaat ccctgatcgc cagaaatcac atgtaacggg cgaccatcgc
    51001 tcatctgtag ttgatagcga cgagagttcg acgcgttcag cagtcgcaag cgcacccagc
    51061 cacgcgagac ttcaacgtac gggctttgta caccgttaac cagcagcgta tcaccaacaa
    51121 agccgccgct tcccggttcg ttgtattctg gcgtaccaaa gttatccagc cgtttatcct
    51181 ggataatgac cggaaaatca tccacaccat aatggttggg gataggcagc gacttgctga
    51241 cttcatcttc caccagccac attccggcaa ggccgttata gacctgctga gccgtgcggt
    51301 tgggagtatt ggcgtgatac cacagagtag ctgcgttctg gcgaataggc agtacgggtg
    51361 cccagtcagc gtttggcgac atcatccgtg ccggaccgcc catcagcggg cctggtacct
    51421 gtagcccggc caccgtcatt gagacatttt ctgtcaggcg gttgctgtaa ataagcttaa
    51481 catcgtcgcc cttccagacg cggatagtcg gccccaggta acgaccattg attccccaga
    51541 ccgacgcgcg tgtccctggc gtaaatgacc agtgcgcacg ttgtacagtc ataaacagcg
    51601 gttgcccacg gcgagattca agtagcggcg gaacgggtag cggttgctgt tgcccggctg
    51661 cgctggcctt caggggaaca gcgcctgcac aaagtgcaat ccccgatgcc tgaatgaact
    51721 gacgccgact gagtgacata tttgctccat gtaaaactgg ctaaagtaat accgtgcagg
    51781 aaagtgttcc ccgcaaaaat acagattgcc gacttaaact tttccggcgg cttcgcgttc
    51841 tgcgacttct ttatcgagct cggcgatttt ttgttccatt atcgaacgac aatgggcagc
    51901 cagctcacga acctgatctt tgccatactg actgacgtca attggcggca gcatttcgac
    51961 aatcaccaga ccgttgtgca gtcgattaag attaatctta ttcgaagttg tagagacgca
    52021 cacgggaata atcgggacgc ccgccgcaat tgccgcgtga aatgctccag tcttgaacgg
    52081 tagcaggccg cgaccacggc tgcgggttcc ttccgggaac atccagatgg aaatacggcg
    52141 ttttttgaaa tgattcacga cttccgcaat ggtgccgtga gctttagtgc gattgtttct
    52201 gtcgatcaat aagttgccgg ttaaccagta caactgcccg aagaagggga tccacagcaa
    52261 gctcttttta cctaccgtca ccgtcggcgg ttgcacaatg ttcgatgctg tcaccatgtc
    52321 atagttgttc tggtggttag cgatatagat agcattgccg tagctttcag catctgcagg
    52381 tttgcggcac tcaactttca ggccgaacag tggcgcaaga cggccaaaca tatgcccaaa
    52441 ggtggccaca tgtttcgggt tacgcgggct gaaaaggcag taaatggagc cgaatacaca
    52501 gactaagatg ctgtaaatca cggtaataat aagacgaaag atatatagca taacaccctc
    52561 tgaaggtctg agagactggc attgtacgtc acctgaaatc aggttagggc attgttaaaa
    52621 gccgctctct ggaaaagcgt attgttaatc gcaacgcatg aataaacaac ggtttcaagg
    52681 gaatttttat cgtttccggg ggttgaatac gctgccggat gacgacttaa cgtttcatcc
    52741 ggcgttcctt gctagcggga ggaaacagcg ccctccccgg catattactc ttcgctatca
    52801 ccgctgctgg cacggcgagg agagtcgatc tcaacacgat cgatacgctg caaaccgcgc
    52861 atcaacgtac cgcggcgtcc acgttcgcca gtgactttct gtagctcttc cggacgcagt
    52921 ttaattttgc gtttcccaac atgaatggtc agcgtgcttt gcggcggcag aacgtacagt
    52981 tgcgccagac catcctcgcc acgcgcggct tctgccgatg gaatgttgat aatcttgttg
    53041 cctttgccct tcgacagctg cggcagatcg cttaccggga acatcaacat acggcctgcc
    53101 tgagtgattg ccagcagcat atcggaagca tcttcaatca ccaccggcgg cataacatgg
    53161 gcattttccg gtaaggtgat caaagcctta cccgcacggt tacgcgctac cagatcgtta
    53221 aaggtgcaga cgaaaccgta acccgcatcg gaagccatca gcagtttctg atcgtcgctt
    53281 tccatcagca tatggtcaac ggtcgcccca ggcggcaacg ttaatttgcc ggtgagtggt
    53341 tcgccctgac cacgcgccga cggcagcgta atcgggtcga tggcatagct acgaccggtg
    53401 gaatcaacaa acactaccgg ttggttactc ttacctttca ccgccgcttt gaagctatca
    53461 cccgctttat aattcaggcc cggcgcgtcg atatcatggc ctttagcgct gcgtacccag
    53521 cccatctgcg acagcacaat ggtgacaggt tcagacggca gcatgtcgtg ctcgctcatc
    53581 gctttcgctt cttcgcgttc ctgcaacggc gaacgacgat cgtcaccgta ggcttgcgcg
    53641 tctgcctgca gttctttctt cagcaggtta ttcattttac gctcggaagc caaaatgccc
    53701 tgcaactggt cgcgctcttt ttccagctca ctctgctcac cgcgaatctt catctcttcc
    53761 agtttggcaa gatgacgcag tttcagttcg aggatcgctt ccgcctgggt ttccgtaagg
    53821 ccaaaccgcg acatcagcgc cggtttcggt tcatcttcgt tacgaatgat ctcaatcact
    53881 tcgtcgatgt tgagaaacgc caccagcaaa ccttccagga tatgcaggcg cttgaggact
    53941 ttctccagac gatagttcag tcggcggcgc acggtatcgc gacggaacac cagccattcg
    54001 gagaggattt ccagcaggtt tttcaccgcc ggacgaccat ccagaccgat catattgagg
    54061 ttaatgcgat agctcttttc cagatcggtg gtagcgaaga ggtggttcat cacctgatcc
    54121 atatccacgc ggttggaacg cggcacaatc accagacggg tcgggttctc gtggtcagat
    54181 tcatcgcgca gatcgtcaac catcggcagc tttttgttgc gcatttgcgc agcaatttgc
    54241 tccagtacgc gcgcacctga aacctgatgc ggtaatgcgc tgataaccac cgcgccatct
    54301 tctttcttcc acaccgcgcg catacgcact gaaccacgtc cgttctcgta gattttacga
    54361 atctcggcgc gcgaagtgat aatttccgct tcagtcggat aatccggccc ctgcacgata
    54421 tccagcagct gatcgagcgt agttttcggc tggtcgatta atgcgattgc cgcctgagct
    54481 acttcacgca ggttatgcgg tggaatatcg gtcgccatgc cgacggcaat accggtggtg
    54541 ccgttaagca aaatgtttgg cagacgggca ggtagcattt tcggctcctg caaagtgccg
    54601 tcgaagtttg gcacccagtc agccgtcccc tgccccagct cgctcaatag cagctcggaa
    54661 tatttcgaca accgggattc ggtgtaacgc attgccgcga acgatttcgg atcgtccggc
    54721 gcgccccagt tcccctgacc atcaaccagc ggataacggt aagagaacgg ctgcgccatc
    54781 aggaccatcg cttcataaca ggcgctatcg ccgtgcggat ggtatttacc cagtacgtca
    54841 ccgacggtac gggccgattt tttaaatttg gcgctggcat tcaggcccag ttcagacatc
    54901 gcatacacaa tgcggcgctg aacaggtttc agaccatcac caataaacgg caacgcacgg
    54961 tccatgatca cgtacatgga gtagtttaag taggcgtttt ccgtaaattc atgtagcgca
    55021 aggcgctctg ccatatcgct cattaattct gattcctcaa cttattcgcc agccttcaaa
    55081 ggggcaatat tgccggagat actacattat ctgccgcgtc gagtcacagg agatcagcgt
    55141 gaatagcgcc gccggacgtg ctccgacggc gataaaggca tcaatgtgcc ttgatataca
    55201 actctttgct gtagacttga tcggcataga gggcagcaca aggttgcgcc catgccacac
    55261 cactcggcat gggggtccgc tccgggcgat tcgagaagtt gaggttatgc gagtccaggg
    55321 aagcctaaag catcaaagta gtcgactgta agtatacggt tcagcccagt ttagctgtgt
    55381 ctccaccagt gccccgcgcc tcctgccata agtgcggctt gctcatggat atccccgaga
    55441 cggcttacgg cgcaccggat aataaatttc gcgtatacat ggtattcctt accggagaaa
    55501 ttataagccc aacaacaata cgcctgtgac attgttcgct caaacttatc gcgagtgatt
    55561 tggctcacac tccggtaaag attccccatc acgccaacgg cacataaaca tccgtttcca
    55621 gtcgctgctc tgtcacccct tcggcaagat tggtgtagtg aaacagaatc ggtgctttac
    55681 gcattttctc gccgcttgca ggcagccagt ggcgaataat gccccatatc gtgtgtgaaa
    55741 tatcgtccag ctcgccaacg tggcgggcca cggcataacg tccaccggta agttcaccat
    55801 tgctcacacc ataacgatta tcgggaatcg gttcgctaac gctgccgcag atatcaaagc
    55861 gaaacgcttc cggtgcggtg gtggcagggg cgtcccaggc gacgccaaaa gtctggcttt
    55921 ggttaacagg agaaagcccc gtctctttac gccacatgat aaatttcgcc acactgtagt
    55981 tgagcaactc agggctacag cggtgcggca acatcgccac agggattgat gggaaatcga
    56041 taatgtttac atccagtgtc aggtttgtca ttttgcctcc ctgcgaaatt attcccggga
    56101 acgcgtcccg ggagcggtaa caattacgga tttactttgc ggatctgttt gacgtcaatt
    56161 tcaacagaat tccagtcttt atcgacttca ccctgaatct caaccgtatc tttcggcgtc
    56221 accgtcacac cgttccagcg tttgtgatcg atatcaacat tgatagtacc gctggcatct
    56281 ttaaacacgt agagatcgtc agagatgcgt tcaacgatat tgccgcgcag ggttacccag
    56341 gtgtcgtcac gcagggattt tgcgctttct acagtcgtta cgctgccgtt cggcccctga
    56401 aatcctccgg cctgactttg cgttaccgat gggccagaaa aaccgccctg ctctgctgcc
    56461 atcaccggtg cactgcacag ggccattact gcgattactg ctgcgaattt tttcatgttt
    56521 attactccct ttaatgtctg tttccgagca tttaacaaga tagtccttaa caacttctta
    56581 agggaaaaaa ataaaattta atgctgtaca gagcgcgtta caacacggtt tactggcagc
    56641 aaatacggtt atcgcaggga tgaaaaaatg cgaattttac tgatagaaga tgacatgctg
    56701 attggtgacg gcatcaaaac tggccttagt aaaatgggtt ttagtgtcga ctggtttaca
    56761 caaggtcgtc agggaaaaga ggcgctttat agcgcacctt atgatgcggt gatcctggat
    56821 ttaaccttac caggcatgga tggtcgcgat attttgcgcg aatggcgaga gaaaggtcag
    56881 cgtgagccgg tactgaacct gaccgcgcgt gatgcgttag cggaacgtgt agaagggctg
    56941 cgtctgggag ctgacgatta tctgtgtaaa ccttttgcgt tgatagaagt cgccgccagg
    57001 ctggaggctc tgatgcgccg aaccaacggc caggccagca acgagctgcg ccacggtaac
    57061 gtcatgctcg accccggcaa acgtatcgcc acgctggctg gcgaaccctt aacactgaaa
    57121 ccaaaagaat ttgccctgct ggaattactg atgcgtaacg ctggtcgggt actgccgcgc
    57181 aaactgattg aagagaaact gtatacctgg gacgaagagg tcaccagtaa tgccgttgaa
    57241 gtgcatgtgc atcatctgcg acgcaaactc ggtagtgatt ttattcgtac cgtgcatggt
    57301 attggctaca cattaggtga gaaatgaaat ttacccaacg tcttagtctg cgcgtcaggc
    57361 tgacgctaat ctttttaatt ctggcctcgg tgacctggct gctttccagc tttgtcgcct
    57421 ggaaacaaac aacggataac gtcgatgaat tgttcgacac ccaactgatg ctgtttgcca
    57481 aacggttaag tacgctcgat ctcaacgaaa tcaacgcggc ggatcgcatg gcacagacgc
    57541 caaataaatt aaaacacggt catgttgatg acgatgcgct gacctttgcc atctttaccc
    57601 acgacggcag aatggtcctt aatgatggcg ataacggaga agatattccc tatagctatc
    57661 aacgggaagg ttttgctgac gggcaactgg tcggtgaaga cgatccttgg cgttttgtct
    57721 ggatgacctc acctgatggc aaatatcgca tcgttgttgg ccaggaatgg gaataccgtg
    57781 aagacatggc gctggcgatt gttgccgggc aattgatccc gtggctggtc gcactgccga
    57841 ttatgttaat catcatgatg gtactactgg gtcgtgaact cgcgccgctg aacaaactgg
    57901 cgctggcact acgtatgcgt gaccctgact cggaaaaacc actaaacgcg actggcgtac
    57961 ccagcgaagt gcggccactg gttgagtcgc taaatcaact gttcgcccgc acacatgcga
    58021 tgatggttcg agaacgacgc tttacctccg atgcagctca cgaactacgt agcccgttaa
    58081 cagcgctgaa agtgcaaacc gaagttgcgc agctctctga cgatgatccg caggcgcgga
    58141 aaaaagcact gctccaatta cattccggga tcgatcgcgc tactcgtctg gttgatcaac
    58201 tgctcacgct atcgcggctg gactcactgg ataaccttca ggacgtcgcg gagatcccgc
    58261 ttgaagatct cctacaatcg tcggtgatgg atatttacca cacggcgcag caggcgaaaa
    58321 ttgacgtgcg actgacactc aatgcccacg gcatcaaacg caccggacaa ccgctattgc
    58381 taagtttgtt ggtgcgaaat ttgctggata acggccccgg tgtgacacca gaggcactgg
    58441 cgcgaattgg cgaacgcttc tatcgcccac ccggacaaac cgctaccggc agtggacttg
    58501 ggctatcgat tgtccagcga atcgccaagc tgcatgacat gaatgttgag tttgggaatg
    58561 cggaacaagg tggatttgag gcgaaggtaa gctggtaact tctctgtcta tgagagccgt
    58621 taaaacgact ctcatagatt ttactaatag caaaatataa accgtcccca aaaaagccac
    58681 caaccacaaa ccagacaaat aacgagcgag aatacatata gccaaaactc gaacatttcc
    58741 cgcataaaga gttttcttaa gataagaata ataagtggcg taagaagaaa aaatgctgca
    58801 atcattgctt gcaatatacg atcttcgcct gaaacggaag gtatactgca aatattgcct
    58861 cccgactcaa gcatccagtc atacttattg aaagcaaaaa aagaatctat taaccaataa
    58921 gtaagcatta tatatggcaa tgcttcaaat attgttttaa ttttctgctt taacatatat
    58981 cagtacgctc ataaaaatcc atttataatt atcaacatga cacgttgata ttttcatctt
    59041 ttacttattc aaagccatat tttcaatccc caaaaaaacc gaaccaaacg tgtaaattat
    59101 tccggtgcga gtaagaatta tgaggaatgg ctatcagtat tgtcattttc agaaaatatt
    59161 tatcctgcat cggtgagtca gagctagatc agacttttgc taaattcgca aaagactttg
    59221 cacattttgc taatttcaca gtaccgctct gtgacgtact atagtcgaca aacgtctcac
    59281 cttgagatta aaaaatgagc aacatcctga ttatcaacgg cgcgaaaaaa ttcgcccact
    59341 ccaatggtca actgaacgac accctgaccg aagtcgcgga tggcacactg cgcgacctcg
    59401 ggcatgatgt ccgcatcgtt cgcgccgaca gcgactacaa tgtcaaagcg gaagtacaaa
    59461 actttctctg ggctgatgtg gtgatctggc agatgccagg ctggtggatg ggcgcgccgt
    59521 ggacagtgaa aaaatatatt gatgatgtat tcaccgaagg tcacgggact ctgtatgcca
    59581 gcgatggtcg tacccgcaaa gatccgtcga aaaaatacgg ttccggcagc ctggtacagg
    59641 acaaaaaata tatgctttct ctaacctgga acgcaccaat ggaagccttc accgaaaaag
    59701 atcagttctt ccacggcgtt ggcgttgacg gtgtgtatct gccgttccat aaagcaaacc
    59761 aattcctcgg tatggaaccg ctgccgacat ttatcgctaa tgacgtgata aaaatgcctg
    59821 atgttccccg ctatactgaa gaatatcgca agcatcttgt ggaaattttt ggttaactag
    59881 agctcaggct ttagaaggag ttaaccatgc ttaccgtaat cgcagaaatc cgtactcgtc
    59941 ctggtcaaca tcaccgtcag gcggtattgg atcagtttgc taaaatcgtt ccaaccgtac
    60001 tgaaagaaga aggttgccac ggctatgcgc caatggtgga ttgcgctgct ggcgtgagtt
    60061 tccagtctat ggcaccggat tctatcgtga tgattgagca gtgggaaagc atcgcgcacc
    60121 ttgaagcgca tctgcaaacc ccgcacatga aggcgtacag cgaagccgta aaaggcgacg
    60181 tgctggagat gaatatccgt attctgcagc cagggattta atcctgcctt gtttgcccgg
    60241 ccatcctgac cgggcaatgt tctttccttt aaacctcaat ctccgccatg tcgcctttct
    60301 cttgcaacca gttgcggcga tcttccgagc gtttcttcgc cagcagcata tccatcatcg
    60361 cgtcagtacg ctgatcgtct tcatcatcga tagtcaactg caccagacgg cgagtgttcg
    60421 gatcaagcgt ggtttcacgc aattgcatcg ggttcatttc ccccagacct ttaaaacgct
    60481 ggacgttcgg cttgcctttc ttgcgtttta attgctcaag tacgccctct ttctcttctt
    60541 ccgtcagcgc gtaataaacc tctttcccga gatcaatacg gtagagcggt ggcagtgcga
    60601 cgtaaacgtg accgtgtttc accaacgcgc ggaagtgttt tacgaacaaa gcgcagagca
    60661 gcgtggcaat gtgcagacca tcagagtccg catccgcgag gatacagatt ttgccataac
    60721 gaagctggct cagatcgtcg ctgtcaggat cgataccgat cgctaccgaa atatcatgca
    60781 cttcctgcga ggccagcact tcgtcggaag aaacttccca ggtgttaagg atcttacctt
    60841 tcagcggcat gatcgcctga tattcacgat cgcgcgcctg cttggcagat ccgcctgcgg
    60901 agtcaccttc cacgaggaac agttcggtac ggttaaggcc ctgcgcggta caatcagcca
    60961 gtttgccagg cagcgccggg ccgctggtca gctttttacg caccactttt ttagccgcac
    61021 gcatacgacg ctgggcgctg gaaatcgcca tctctgccag caactccgcc gcctgaacgt
    61081 tctggttcag ccacaggatg aaggcatctt tcaccacgcc cgaaacgaat gccgcacact
    61141 gacgcgaaga gagacgctct ttggtctgcc cggcaaactg cggatcctgc attttaaccg
    61201 acagaacata ggcgcagcga tcccagatat cttccgccga cagcttcacg ccgcgcggca
    61261 gaatgttgcg gtattcacag aactcacgca tcgcgtccaa caggccctga cgcagaccat
    61321 taacatgggt accgccctgc atcgttggga taaggttgac gtagctttcg gtcagcagtt
    61381 caccgccttc cggcagccac agtagcgccc agtccacagc ttcagtatcg ccagcgaaat
    61441 taccgataaa cggtttttct ggcagcgtcg gtaaaccgtt taccgcttcc gccaggtaat
    61501 cattcagacc gtcctgatag caccagcgct gttcggtgtt gttgatctca tctttaaagg
    61561 taatctcaac gccggggcac agtactgctt tggccttcag cacatgcgtc aggcgtgaaa
    61621 cagaaaaacg cgggctgtca aagaaggttt catccggcca gaagtgcacg ctggtaccgg
    61681 tattgcgttt accgcaagtg ccgacaacct gtaaatcctg caccttttcg ccattttcaa
    61741 aggcgatgtt ataaacctga ccatcgcggc gcacgttaac ttctacgcgc ttcgacaagg
    61801 cgttaactac cgaaatcccc acgccatgca ggccgccaga gaactggtaa tttttgttag
    61861 agaatttacc gcctgcatgc agacggcaaa gaatcagttc aaccgccggt actccttctt
    61921 ccgggtgaat atccaccggc atcccgcgcc catcgtcaat cacttctaac gactggtcag
    61981 catgtaagat tacatccacg cgttttgcgt gacccgccag tgcttcatcg acactgttat
    62041 caataacttc ttgccccaaa tggttagggc gagtggtatc ggtatacatc cccggacggc
    62101 ggcgaaccgg ctcaagcccg gtgagtacct caatggcatc agcgttataa gtttgcgtca
    62161 tggtttaagt tagcaattcg agttgatcgt cagagatggt gcagaccaag aaaatcgacg
    62221 atcgggttga aataatcttc gaagcccgtg aatgcgtggt tgccgccttc tatgacagtc
    62281 tggcggcagg aagcatagta cgccaccgcc tggcggtaat ccagcacttc atctcccgtc
    62341 tgttgcagca gccagatcaa atccggcgct tccagcgggt caatctgcat gactttgaga
    62401 tcgtaaatat ggcgtgactc tagcacatat tgctgcccgg tgtaggggtt ctcgttctga
    62461 ccgagatagt ccgtcagcaa ttcaaacggg cgtaccgccg ggtttaccac cactgcgggc
    62521 agcataaaac attgcgacaa ccaggtggcg taatatcccc ccagtgacga accgacaata
    62581 cccagcgaat cgccgccatg ttccaggaca atggattcca gcagttctgc cgcgtcggaa
    62641 ggatacggcg gcaactgcgg aatgatcatc tcaacgtcag ggtgatgttc cgccagccag
    62701 ttttttaaca agctcgcttt tgcagagcgc ggcgagctgt tgaaaccgtg taaataaaga
    62761 agcgtagaca tcagtagcct tctgaagcgg tatcaggttg gaaacgtgtg tccgccaggc
    62821 gatgcacctc ggtggtcagc gtgccatcag catgtaactc gagagtacgc cagccgggcg
    62881 cgatagtatc cagcgtaaag ttggaacagt gcggcttaaa ctgcacacag gtcgacggcg
    62941 ttgccagcag gcggcgaccg ttccagtcga gatccagctc ctgatgaata tgaccgcaca
    63001 gcaagtattt gacgtgcgga aacttcgcca gcacggtatc cagttcgccc gcgttacgca
    63061 gactgtgttg atcgagccaa ctacaacccg caggtagcgg atgatgatgc agcagcagca
    63121 acgtatggcg ttctggcgca tcggccagtt tacgttccag ccactcaagc tgaaactcgc
    63181 tcagctcacc gtgcggcacg ccaaacacct ggctatccag caacaggatt tgccattgct
    63241 caccaataaa cacccgcttc gccggggaga tacccgcatc ctgtaacgcg ctgtacatcg
    63301 cgggctggaa atcgtggttg cccggcagcc agacgcaggg cgcacgaaaa cttgcgatgc
    63361 cttcagcgaa atgctgatag gccgcagagg attgatcctg cgctaaatca cctgtcgcga
    63421 caatcaggtc gaattcgtgc tggtgtggac gaatcgcctc cagcaccgcc tggtagcttt
    63481 cccaggtgtt tacccctaac agggcttcgt gcttttgtgc aaacaggtga gtatcggtaa
    63541 tttgtaaaat ccttcctctg gcctcaccag ccagaggaag ggttaacagg ctttccaaat
    63601 ggtgtcctta ggtttcacga cgctagtaaa ccggaatcgc catcgctcca tgtgctaaac
    63661 agtatcgcaa ccagtccgct aaaaactggt taatttgatg cttttcgtcg cgttgatgca
    63721 actttttatt aggataatca taccgcgctt tgaagcgaaa aatctgctgg cttgaacaca
    63781 cttcagccac catcgcgtca tgatacagac gcaccgtcat tgacggaagg ctccagtaac
    63841 tgatcgcggg cgcagtctgt tctattgtca ccagggtagt gtatcgggtc gattccacaa
    63901 tcgtcagccg atattgtgcg tttgccacct gatagcttac agtttcgccg ggtgcgtcat
    63961 tgcgcggtaa caaacggcgc aattgtgaaa agttcatctc gcacaggcgc atcatttcag
    64021 gaaagtcagg tgtgtaacgc ttcatttatg cccactcatt ttttaacgct tgatgatgca
    64081 gctgcagcca ttgcaaagcg atgaccgacg ctgcgttgtc gattttcccc tcttctaccc
    64141 actggtatgc ctgttcccgg cttaccacat gaacgcgaat atcttcgttt tcatcagcca
    64201 gaccatgaat accgcttgcg gtcgtggcgt ccacttcgcc caccataatt gacgaacgct
    64261 cactggtgcc ccccgggctt gccaggaaac ttaacaccgg tttggtccgt ttgactatca
    64321 gtcccgcctc ttcaatcgct tcgcgacggg caacatcttc cacactttca ccctcttcaa
    64381 tcatcccggc aaccatctcc agtagccagg gggtttcgct ggtgtcgtac gcggcaatcc
    64441 gaatctgctc aatcagcaca acttcatcac gcactgggtc aaagggtagc aagactgcgg
    64501 cgtgaccgcg ctcaaaaatt tcccgccgta cctcatgact catttgcccg ttgaatagac
    64561 gatgacgaaa tctataaaga tctaatgaaa aaaagccgcg ataaagtgtt tctcgtgcaa
    64621 taatttctac atcgtttttg ccaaatgtaa cgggcaggtt gtctggctta agcattgtta
    64681 atgtcctagc actaatagtg aattaaatgt gaatttcagc gacgtttgac tgccgtttga
    64741 gcagtcatat gttaaattga ggcacattaa cgccctatgg cacgtaacgc caaccttttg
    64801 cggtagcggc ttctgctaga atccgcaata attttacagt ttgatcgcgc taaatactgc
    64861 ttcaccacaa ggaatgcaaa tgaagaaatt gctccccatt cttatcggcc tgagcctttc
    64921 tgggttcagt tcgttgagcc aggccgagaa cctgatgcaa gtttatcagc aagcacgcct
    64981 tagtaacccg gaattgcgta agtctgccgc cgatcgtgat gctgcctttg aaaaaattaa
    65041 tgaagcgcgc agtccattac tgccacagct aggtttaggt gcagattaca cctatagcaa
    65101 cggctaccgc gacgcgaacg gcatcaactc gaacgcgacc agtgcgtccc tgcagttaac
    65161 tcaatccatt tttgatatgt cgaaatggcg tgcgttaacg ctgcaggaaa aagcagcagg
    65221 gattcaggac gtcacatatc agaccgatca gcaaaccttg atcctcaaca ccgcgaccgc
    65281 ttatttcaac gtgttgaatg ctattgacgt tctttcctat acacaggcac aaaaagaagc
    65341 gatctaccgt caattagatc aaaccaccca acgttttaac gtgggcctgg tagcgatcac
    65401 cgacgtgcag aacgcccgcg cgcagtacga taccgtgctg gcgaacgaag tgaccgcacg
    65461 taataacctt gataacgcgg tagagcagct gcgccagatc accggtaact actatccgga
    65521 actggcggcg ctgaatgtcg aaaactttaa aaccgacaaa ccacagccgg ttaacgcgct
    65581 gctgaaagaa gccgaaaaac gcaacctgtc gctgttacag gcacgcttga gccaggacct
    65641 ggagcgcgag caaattcgcc aggcgcagga tggtcactta ccgactctgg atttaacggc
    65701 ttctaccggg atttctgaca cctcttatag cggttcgaaa acccgtggtg ccgctggtac
    65761 ccagtatgac gatagcaata tgggccagaa caaagttggc ctgagcttct cgctgccgat
    65821 ttatcagggc ggaatggtta actcgcaggt gaaacaggca cagtacaact ttgttggtgc
    65881 cagtgagcaa ctggaaagcg cacatcgtag cgtcgtgcaa accgtacgtt cctccttcaa
    65941 caacattaat gcttctatca gtagtattaa cgcctacaaa caagccgtag tttccgcgca
    66001 aagctcatta gacgcgatgg aagcgggcta ctcggtcggt acgcgtacca ttgttgatgt
    66061 gttggatgcg accaccacgc tgtacaacgc caagcaagag ctggcgaatg cgcgttataa
    66121 ctacctgatt aatcagctga atattaagtc agccctgggt acgttgaacg agcaggattt
    66181 gctggcactg aacaatgcgc tgagcaaacc ggtttccact aatccggaaa acgttgcccc
    66241 gcaaacgccg gaacagaatg ctattgctga tggttatgcg cctgatagcc cggcacccgt
    66301 cgttcagcaa acatccgcac gcactaccac cagtaacggt cataaccctt tccgtaactg
    66361 atgacgacga cggggcttcg gccccgtctg aacgtaaggc aacgtaaaga tacgggttat
    66421 ctgccgcatt cttccccctt ctcgcttcaa tttcgaccag ccatcctcta ttctgatggg
    66481 tatttaccac tggtcccgga agacaaaaat gaaacggaca aaatccatac gccacgcatc
    66541 gttccgcaaa aactggagcg cacgccatct gacaccagtc gctctcgcgg ttgccactgt
    66601 ttttatgctg gctggctgtg aaaagagtga tgaaacagtg tctctctatc aaaatgctga
    66661 cgactgttca gctgcaaacc caggcaaaag cgccgaatgt accaccgcgt acaacaatgc
    66721 gctgaaagaa gccgaacgta ctgcgccgaa atacgccacc cgtgaagact gtgttgctga
    66781 atttggtgaa ggtcagtgtc agcaagcacc agcccaggct ggcatggcac cagaaaacca
    66841 ggcgcaggct cagcaatcca gcgggagttt ctggatgccg ctgatggccg gttacatgat
    66901 ggggcgtctg atgggcggcg gcgcgggatt tgcacagcag ccgctgttct cctcgaaaaa
    66961 cccggccagt ccggcttacg gtaaatatac cgacgcgacg ggtaaaaact atggcgcagc
    67021 ccagccaggc cgcaccatga ccgtaccgaa gacggcaatg gcaccaaaac cggcgaccac
    67081 cactaccgtt acccgtggcg gttttggtga atctgttgcc aaacaaagca ctatgcagcg
    67141 tagcgcaacc ggcacctctt ctcgttcaat gggtggctga taccgatgga aagagtcagt
    67201 attaccgagc gcccggactg gcgtgagaaa gcccacgaat acggtttcaa ttttcacacc
    67261 atgtacggcg agccgtactg gtgtgaagat gcttactaca agttgaccct cgcccaggtt
    67321 gaaaagctgg aagaagtcac cgccgaactg caccagatgt gcctgaaagt ggtggaaaaa
    67381 gtgatcgcca gcgatgagct gatgaccaaa ttccgcatac caaaacacac ctggagtttt
    67441 gtgcgtcaat catggctgac tcaccagcca tcgctttatt cgcgtcttga tctggcgtgg
    67501 gatggcactg gcgaacctaa acttctggaa aataacgccg atacgccaac gtcactatac
    67561 gaggcggcgt tctttcagtg gatctggctg gaagatcagc ttaacgccgg taacttgccg
    67621 gagggcagcg accagtttaa cagtctgcaa gaaaaactga tcgatcgctt cgttgagctg
    67681 cgtgaacagt atggcttcca gttgctgcat ctcacctgct gtcgcgacac ggtggaagat
    67741 cgcggaacca ttcagtattt gcaggactgc gcaaccgaag ctgaaatcgc caccgagttc
    67801 ctctacatcg acgagatagg tttaggcgaa aaaggccagt tcacggattt acaggatcag
    67861 gtgatttcta acctgttcaa gctttatccg tgggaattta tgctgcgcga aatgttctcc
    67921 accaagctgg aagatgctgg cgtacgctgg ctggaaccgg cgtggaagag cattatctcc
    67981 aacaaggcac ttctaccgct actgtgggag atgttcccga atcacccgaa cctgctgccc
    68041 gcttattttg cggaagatga tcatccgcaa atggaaaaat atgtggttaa accgatcttc
    68101 tcccgtgaag gcgcaaacgt gtcgatcatt gagaacggca aaaccattga agcagcggaa
    68161 ggtccgtatg gcgaagaagg gatgattgtt cagcaattcc acccgttacc gaaattcggc
    68221 gacagctata tgctgattgg tagctggctg gtgaacgatc aacctgccgg aattggcatt
    68281 cgtgaagacc gtgcgttgat cacccaggat atgtctcggt tttatccaca tatttttgtt
    68341 gaataagcca cgataccgga tggcactcgc catccggtaa ttgttagcct atctgcaccg
    68401 acagcatact taaactgccc atttctatac catcgaccgg aatggtaatt ggctcctgcc
    68461 catcccacac acctaacaca tacaacaacg gcagatagtg ctctggcgtt gggttcgata
    68521 acgcgccacc ttcatggtcg agataattca ccagaggatg ctgctctacc ggcccttgcc
    68581 acgtcagatt cgctttcaca tactcattaa acgacgtcgc ccacggatac ggtgaactat
    68641 caccgtgcca cttcactgtg cgcaggttat gcaccacgtt accgctggcg accaacatta
    68701 ttccttcatc acgcagcgcc gcgagtttgc gccccatttc gaaatgccag gcggcaggtt
    68761 tgctactgtc gatactcaac tgcaccatag ggatatcggc gtcaggatac atcttaatca
    68821 gcacgcccca cgagccgtgg tcaaagcccc aggcttcttt atccagcgtc accgggatcg
    68881 gcgctaacag ctcaaccaga cgctgtgcca gcgcaggcga acccggagca ggataatgcg
    68941 tatcgtacag cgcctgcggg aagccaccaa agtcatgaat cgtgggcggc gtctccatcg
    69001 cggtcactcc tgttccacgg gtaaaccagt gagccgaaac caccacaatc gcttgcgggc
    69061 gtggcaatgt catccccaac ttctgccagc tgcgggtata caaattatct tccagcacgt
    69121 tcatcggact accgtgacct aaaaacaatg ctggcatacg tgttgaagac atgatgatat
    69181 ccttaactaa aggtgtcatt ttgatatcct cacaatacgc ttgttcggcg gagtaagaac
    69241 ccggataaca atgatgatga tcatcagtta ttttgacgat ctgcctgaag gtgaagattt
    69301 ataaggagtt gtcgatgtca gtacctctca ttctgaccat actggcgggg gcagccacgt
    69361 ttattggcgc gtttctcggc gttctcggac aaaaaccctc gaaccgctta ctggcgtttt
    69421 cgctaggttt tgcggcgggg atcatgttgc tcatctcatt aatggaaatg cttcctgccg
    69481 cactagcagc tgaaggaatg tcgccggtgt tgggttatgg aatgtttatc ttcggtctgc
    69541 ttggctactt tggcctggac cgcatgttgc cgcatgcgca tccgcaggat ttaatgcaaa
    69601 aatcggtgca gctgttgcca aaatcgatca agcgcacagc cattctgctc actctcggca
    69661 tcagtctgca taacttcccg gaagggattg ccacctttgt cacggcgagc agcaacctgg
    69721 agctgggatt tggcatcgca ctggccgtcg cgttgcacaa tatccctgaa ggtctggcag
    69781 tggcaggccc ggtttatgcg gcaacggggt ctaaacgcac cgcgattctg tgggcgggga
    69841 tttctggact agcagaaatt cttggtggtg tgctggcgtg gttaatcctc ggtagcatga
    69901 tttccccggt ggtcatggcg gcaatcatgg cggcggttgc aggaattatg gtggcgctct
    69961 cggttgatga attaatgccg ctcgccaaag agattgaccc taataataac cccagctatg
    70021 gcgtactatg tggaatgtca gtgatgggat tcagtttagt gctgctacaa acggcgggaa
    70081 ttggttaaaa acaacatcag aagttgtcca gcacatggac aacttctgcc agcttcacga
    70141 aagccttatt ctaccttttc ggctatctct tctctcgtac tgtttaagta tttgttccgc
    70201 gcttcaagca gtgcgtctct gtcctggcga aacgcccgac aataacgttt caaatgcagg
    70261 aaataaccca ccacaaccag agcaatcaat acaatccagt accaggcgat aaacatcctt
    70321 ttacccttaa tatttaacaa tactaaacat ttagcgcata aatttcacat atcctttttc
    70381 ggatatattc atatggtcgc ataataacgg aacaattata tgattttgat cataatgaaa
    70441 aagaatgcct gatattaatg aaattttctc atagaaagag aagcggaatt aacatcttgc
    70501 atagcagggc aaaataaaaa ccgaagccag ctacaacttc ggtttcttga ttaaggcagt
    70561 aattaagcag cggttttcag ctggctttac gctcatgtgc ctgacggtat gccaccaggt
    70621 cttcaatagt cacgagcgcc atattgtgtt tattggcaaa ctcaatgcac tctggtgcac
    70681 gcgccatcgt gccatcgtca ttagtcagct cacacagcac accagccggt ttaaagcctg
    70741 ccagcgtcat caaatcaata gtcgcttcag tatgaccgcc acgcgtcagt acaccacctg
    70801 cctgcgcgcg aagtgggaaa acgtggcccg ggcgattcag atcagaaggt tttgcgccat
    70861 cggcaatcgc tgcgcgaacg gtcgtaatac gatcagcggc agaaacaccg gtagtcacac
    70921 cttcagccgc ttcaatggtc acggtaaaac cggtgccata ggcgctggtg ttattttcta
    70981 ccatcattgg cagatcaagt tgtttacggc gatcttcagt gatgcacagg caaacaatac
    71041 cgctaccgtg gcgaatggtc agcgccatct gctcaacggt catggtttct gccgggaaga
    71101 tcatatcgcc ttcgttttca cggtcttcat catcaagcac cattacaccg cgtccttcac
    71161 gcagcgcagc cagtgcattt tcaacacgtt cgaaaggcgt accaaaagag gaaagtagcg
    71221 tctgattcat ggtaaaaaaa cctcactaaa attatggtta ccagaatcag ggcagtctta
    71281 ggagtggcgg catatagcca aaataacgtg agcgggtcca tgcccgacag aatcgttact
    71341 ctctcccatc cggactctaa ccgtcggccc cggaattaca ccggatctgc tgtcctttga
    71401 gttcgcaccc aaagcgctcg cgggctttca actgagttga tttaccgccg gtggggaatt
    71461 tcgccccgcc ctgagaataa gcggattcac tataacgcta atgattagcg gcagcaacgc
    71521 atagcttcac ataattctgg tttatgactt acccttatcg cactacaatg gtactcaaca
    71581 cctatcagta cagggaaact acaatgattg acccgaaaaa aattgagcaa atcgctcgcc
    71641 aggttcacga atcaatgcct aaaggaatca gggagttcgg ggaagatgtg gagaaaaaaa
    71701 tccgccaaac cctacaagct cagctgacgc gcctcgacct tgtaagccgt gaagagttcg
    71761 acgtccaaac gcaagtcctg ttacggactc gtgagaaact ggcgctgctt gaacaaagaa
    71821 tcagcgaact ggaaaatcgc agtactgaga tcaaaaagca gccagatcca gagactctcc
    71881 ccccaacgct gtaaacattt gtctttaatg cactgtaaat tatgaattaa tcctctaatt
    71941 tacagtgttt tctattacat tatcgcttaa tcccattcag agtatcatca caaaaatgaa
    72001 tgattgcaat tgaaaatata attgttatgt attctttaag ttttacttaa tcgatat
//
LOCUS       DAJTQQ010000006        69353 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000006 DAJTQQ010000000
VERSION     DAJTQQ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 69353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.006"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..427
                     /locus_tag="OO309_000457"
     CDS             <1..427
                     /locus_tag="OO309_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5845726.1"
                     /translation="DPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNRG
                     AMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISSK
                     SQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            438..1529
                     /locus_tag="OO309_000458"
     CDS             438..1529
                     /locus_tag="OO309_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5845727.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1572..2432)
                     /gene="rsmI"
                     /locus_tag="OO309_000459"
     CDS             complement(1572..2432)
                     /gene="rsmI"
                     /locus_tag="OO309_000459"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR5845728.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2497..4533
                     /gene="lpoA"
                     /locus_tag="OO309_000460"
     CDS             2497..4533
                     /gene="lpoA"
                     /locus_tag="OO309_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR5845729.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4491..4886
                     /locus_tag="OO309_000461"
     CDS             4491..4886
                     /locus_tag="OO309_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR5845730.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4906..5496
                     /gene="diaA"
                     /locus_tag="OO309_000462"
     CDS             4906..5496
                     /gene="diaA"
                     /locus_tag="OO309_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR5845731.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5506..6081
                     /gene="yraP"
                     /locus_tag="OO309_000463"
     CDS             5506..6081
                     /gene="yraP"
                     /locus_tag="OO309_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR5845732.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6195..7235)
                     /locus_tag="OO309_000464"
     CDS             complement(6195..7235)
                     /locus_tag="OO309_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5845733.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7308..7943)
                     /locus_tag="OO309_000465"
     CDS             complement(7308..7943)
                     /locus_tag="OO309_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR5845734.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8071..8589
                     /gene="yhbO"
                     /locus_tag="OO309_000466"
     CDS             8071..8589
                     /gene="yhbO"
                     /locus_tag="OO309_000466"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR5845735.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8569..9012)
                     /locus_tag="OO309_000467"
     CDS             complement(8569..9012)
                     /locus_tag="OO309_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR5845736.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9063..9365
                     /gene="yhbQ"
                     /locus_tag="OO309_000468"
     CDS             9063..9365
                     /gene="yhbQ"
                     /locus_tag="OO309_000468"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR5845737.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9352..9855)
                     /locus_tag="OO309_000469"
     CDS             complement(9352..9855)
                     /locus_tag="OO309_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5845738.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9849..10373)
                     /locus_tag="OO309_000470"
     CDS             complement(9849..10373)
                     /locus_tag="OO309_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR5845739.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10582..11577
                     /locus_tag="OO309_000471"
     CDS             10582..11577
                     /locus_tag="OO309_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5845740.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11586..12464
                     /locus_tag="OO309_000472"
     CDS             11586..12464
                     /locus_tag="OO309_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5845741.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12545..13552
                     /locus_tag="OO309_000473"
     CDS             12545..13552
                     /locus_tag="OO309_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR5845742.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13670..14914)
                     /gene="mtr"
                     /locus_tag="OO309_000474"
     CDS             complement(13670..14914)
                     /gene="mtr"
                     /locus_tag="OO309_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR5845743.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15068..16957)
                     /gene="deaD"
                     /locus_tag="OO309_000475"
     CDS             complement(15068..16957)
                     /gene="deaD"
                     /locus_tag="OO309_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR5845744.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16950..17030)
                     /gene="yrbN"
                     /locus_tag="OO309_000476"
     CDS             complement(16950..17030)
                     /gene="yrbN"
                     /locus_tag="OO309_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR5845745.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17137..18021)
                     /gene="nlpI"
                     /locus_tag="OO309_000477"
     CDS             complement(17137..18021)
                     /gene="nlpI"
                     /locus_tag="OO309_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR5845746.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18130..20265)
                     /gene="pnp"
                     /locus_tag="OO309_000478"
     CDS             complement(18130..20265)
                     /gene="pnp"
                     /locus_tag="OO309_000478"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR5845747.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20512..20781)
                     /gene="rpsO"
                     /locus_tag="OO309_000479"
     CDS             complement(20512..20781)
                     /gene="rpsO"
                     /locus_tag="OO309_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR5845748.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20930..21874)
                     /gene="truB"
                     /locus_tag="OO309_000480"
     CDS             complement(20930..21874)
                     /gene="truB"
                     /locus_tag="OO309_000480"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR5845749.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21874..22275)
                     /gene="rbfA"
                     /locus_tag="OO309_000481"
     CDS             complement(21874..22275)
                     /gene="rbfA"
                     /locus_tag="OO309_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR5845750.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22439..25087)
                     /gene="infB"
                     /locus_tag="OO309_000482"
     CDS             complement(22439..25087)
                     /gene="infB"
                     /locus_tag="OO309_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR5845751.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25112..26599)
                     /gene="nusA"
                     /locus_tag="OO309_000483"
     CDS             complement(25112..26599)
                     /gene="nusA"
                     /locus_tag="OO309_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR5845752.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSAELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26627..27079)
                     /gene="rimP"
                     /locus_tag="OO309_000484"
     CDS             complement(26627..27079)
                     /gene="rimP"
                     /locus_tag="OO309_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR5845753.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27286..27362)
                     /locus_tag="OO309_000485"
     tRNA            complement(27286..27362)
                     /locus_tag="OO309_000485"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27326..27328),aa:Met,seq:cat)
     gene            27710..29053
                     /gene="argG"
                     /locus_tag="OO309_000486"
     CDS             27710..29053
                     /gene="argG"
                     /locus_tag="OO309_000486"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR5845754.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29061..30668)
                     /locus_tag="OO309_000487"
                     /pseudo
     CDS             complement(29061..30668)
                     /locus_tag="OO309_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31146..31232)
                     /locus_tag="OO309_000488"
     tRNA            complement(31146..31232)
                     /locus_tag="OO309_000488"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31196..31198),aa:Leu,seq:gag)
     gene            complement(31247..31579)
                     /gene="secG"
                     /locus_tag="OO309_000489"
     CDS             complement(31247..31579)
                     /gene="secG"
                     /locus_tag="OO309_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR5845755.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(31807..33144)
                     /gene="glmM"
                     /locus_tag="OO309_000490"
     CDS             complement(31807..33144)
                     /gene="glmM"
                     /locus_tag="OO309_000490"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR5845756.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33137..33985)
                     /gene="folP"
                     /locus_tag="OO309_000491"
     CDS             complement(33137..33985)
                     /gene="folP"
                     /locus_tag="OO309_000491"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR5845757.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34075..36009)
                     /gene="ftsH"
                     /locus_tag="OO309_000492"
     CDS             complement(34075..36009)
                     /gene="ftsH"
                     /locus_tag="OO309_000492"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR5845758.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36109..36738)
                     /gene="rlmE"
                     /locus_tag="OO309_000493"
     CDS             complement(36109..36738)
                     /gene="rlmE"
                     /locus_tag="OO309_000493"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR5845759.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            36864..37157
                     /gene="yhbY"
                     /locus_tag="OO309_000494"
     CDS             36864..37157
                     /gene="yhbY"
                     /locus_tag="OO309_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR5845760.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37313..37789)
                     /gene="greA"
                     /locus_tag="OO309_000495"
     CDS             complement(37313..37789)
                     /gene="greA"
                     /locus_tag="OO309_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR5845761.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38037..39470
                     /gene="dacB"
                     /locus_tag="OO309_000496"
     CDS             38037..39470
                     /gene="dacB"
                     /locus_tag="OO309_000496"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5845762.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(39656..40828)
                     /gene="cgtA"
                     /locus_tag="OO309_000497"
     CDS             complement(39656..40828)
                     /gene="cgtA"
                     /locus_tag="OO309_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR5845763.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(40844..41809)
                     /locus_tag="OO309_000498"
     CDS             complement(40844..41809)
                     /locus_tag="OO309_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5845764.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(41936..42193)
                     /gene="rpmA"
                     /locus_tag="OO309_000499"
     CDS             complement(41936..42193)
                     /gene="rpmA"
                     /locus_tag="OO309_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR5845765.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42214..42525)
                     /gene="rplU"
                     /locus_tag="OO309_000500"
     CDS             complement(42214..42525)
                     /gene="rplU"
                     /locus_tag="OO309_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR5845766.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            42783..43754
                     /gene="ispB"
                     /locus_tag="OO309_000501"
     CDS             42783..43754
                     /gene="ispB"
                     /locus_tag="OO309_000501"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR5845767.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            43981..44259
                     /gene="sfsB"
                     /locus_tag="OO309_000502"
     CDS             43981..44259
                     /gene="sfsB"
                     /locus_tag="OO309_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR5845768.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44307..45566)
                     /gene="murA"
                     /locus_tag="OO309_000503"
     CDS             complement(44307..45566)
                     /gene="murA"
                     /locus_tag="OO309_000503"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR5845769.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45621..45875)
                     /gene="ibaG"
                     /locus_tag="OO309_000504"
     CDS             complement(45621..45875)
                     /gene="ibaG"
                     /locus_tag="OO309_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR5845770.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46035..46328)
                     /gene="mlaB"
                     /locus_tag="OO309_000505"
     CDS             complement(46035..46328)
                     /gene="mlaB"
                     /locus_tag="OO309_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR5845771.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46328..46963)
                     /gene="mlaC"
                     /locus_tag="OO309_000506"
     CDS             complement(46328..46963)
                     /gene="mlaC"
                     /locus_tag="OO309_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR5845772.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(46982..47533)
                     /gene="mlaD"
                     /locus_tag="OO309_000507"
     CDS             complement(46982..47533)
                     /gene="mlaD"
                     /locus_tag="OO309_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR5845773.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47538..48320)
                     /gene="mlaE"
                     /locus_tag="OO309_000508"
     CDS             complement(47538..48320)
                     /gene="mlaE"
                     /locus_tag="OO309_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR5845774.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48328..49137)
                     /gene="mlaF"
                     /locus_tag="OO309_000509"
     CDS             complement(48328..49137)
                     /gene="mlaF"
                     /locus_tag="OO309_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR5845775.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49347..50312
                     /locus_tag="OO309_000510"
     CDS             49347..50312
                     /locus_tag="OO309_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR5845776.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50326..51312
                     /gene="kdsD"
                     /locus_tag="OO309_000511"
     CDS             50326..51312
                     /gene="kdsD"
                     /locus_tag="OO309_000511"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR5845777.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51333..51899
                     /gene="kdsC"
                     /locus_tag="OO309_000512"
     CDS             51333..51899
                     /gene="kdsC"
                     /locus_tag="OO309_000512"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR5845778.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            51896..52471
                     /gene="lptC"
                     /locus_tag="OO309_000513"
     CDS             51896..52471
                     /gene="lptC"
                     /locus_tag="OO309_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR5845779.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52440..52997
                     /gene="lptA"
                     /locus_tag="OO309_000514"
     CDS             52440..52997
                     /gene="lptA"
                     /locus_tag="OO309_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR5845780.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53004..53729
                     /gene="lptB"
                     /locus_tag="OO309_000515"
     CDS             53004..53729
                     /gene="lptB"
                     /locus_tag="OO309_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR5845781.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            53777..55210
                     /gene="rpoN"
                     /locus_tag="OO309_000516"
     CDS             53777..55210
                     /gene="rpoN"
                     /locus_tag="OO309_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR5845782.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55233..55520
                     /gene="hpf"
                     /locus_tag="OO309_000517"
     CDS             55233..55520
                     /gene="hpf"
                     /locus_tag="OO309_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR5845783.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            55638..56129
                     /gene="ptsN"
                     /locus_tag="OO309_000518"
     CDS             55638..56129
                     /gene="ptsN"
                     /locus_tag="OO309_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR5845784.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56175..57029
                     /gene="rapZ"
                     /locus_tag="OO309_000519"
     CDS             56175..57029
                     /gene="rapZ"
                     /locus_tag="OO309_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR5845785.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57026..57298
                     /gene="npr"
                     /locus_tag="OO309_000520"
     CDS             57026..57298
                     /gene="npr"
                     /locus_tag="OO309_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR5845786.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57511..58143
                     /gene="yrbL"
                     /locus_tag="OO309_000521"
     CDS             57511..58143
                     /gene="yrbL"
                     /locus_tag="OO309_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR5845787.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58140..58867)
                     /gene="mtgA"
                     /locus_tag="OO309_000522"
                     /pseudo
     CDS             complement(58140..58867)
                     /gene="mtgA"
                     /locus_tag="OO309_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(58864..59517)
                     /gene="elbB"
                     /locus_tag="OO309_000523"
     CDS             complement(58864..59517)
                     /gene="elbB"
                     /locus_tag="OO309_000523"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR5845788.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(59747..62083)
                     /gene="arcB"
                     /locus_tag="OO309_000524"
     CDS             complement(59747..62083)
                     /gene="arcB"
                     /locus_tag="OO309_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR5845789.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62179..63108)
                     /locus_tag="OO309_000525"
     CDS             complement(62179..63108)
                     /locus_tag="OO309_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR5845790.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            63783..68243
                     /gene="gltB"
                     /locus_tag="OO309_000526"
     CDS             63783..68243
                     /gene="gltB"
                     /locus_tag="OO309_000526"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR5845791.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68256..>69353
                     /gene="gltD"
                     /locus_tag="OO309_000527"
     CDS             68256..>69353
                     /gene="gltD"
                     /locus_tag="OO309_000527"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR5845792.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVR"
BASE COUNT        16963 a        18836 c        17216 g        16338 t
ORIGIN      
        1 tgatcccttt ggctatgcag tggttcctta tctcacgact tatcaggaaa accgtctctc
       61 ggtagatact acgcagctgc ccgataacgt cgatcttgaa caaacaacac agtttgtggt
      121 gcccaacaga ggtgcaatgg tagcggcgcg tttcaacgcc aatatcggtt atcgcgtact
      181 tgttacagtc agcgatcgca acggtaaacc gttgcccttt ggcgctcttg ccagcaacga
      241 tgatacgggg caacaaagta tcgtcgatga gggcggcata ctatatctct ctgggatatc
      301 gagtaaatca caaagctgga ctgtacgctg gggaaatcag gcagatcaac aatgtcagtt
      361 tgcttttagt acaccggatt cagaaccaac aacctctgta ttacaaggca cagcgcagtg
      421 ccattaagga taaaaaaatg aaaagagcgc ctcttataac aggacttttg ttgatatcca
      481 catcctgcgc ttatgcctcc tcaggagggt gtggagccga cagcactagc ggtgcgacaa
      541 attacagcag tgtggttgat gatgttacgg tgaaccagac agataacgtg acaggacggg
      601 agtttacctc tgcaacgcta agtagcacta actggcaata cgcctgttcc tgctctgcgg
      661 gtaaggcagt taaacttgtc tatatggtca gccccgtact taccaccact ggacatcaga
      721 caggatatta caaactcaat gacagcctgg atattaaaac cacattacag gcaaacgaca
      781 ttccaggact cacaaccgac caggttgtct ctgttaacac ccgattcaca cagataaaaa
      841 gcagcaccgt atattctgct gcaacccaaa cgggtgtttg ccagggtgac acgtctcgtt
      901 atggacccgt taatattggt gcaaatacca cctttaccct gtatgtcacc aagccatttc
      961 tcggctcgat gaccattccg aaaacggata ttgccgtcat taaaggcgcg tgggtcgatg
     1021 gaatgggaag cccgtctaca ggtgacttcc atgatttagt caagttatcg attcagggaa
     1081 atctcaccgc cccacagtcg tgcaaaatta atcagggcga tgttattaaa gttaattttg
     1141 gattcatcaa tggtcagaag tttaccaccc gcaatgccat gccagacggt tttactccag
     1201 tagactttga tatcacttat gactgtggtg atacttcaaa gattaaaaac tcgttgcaaa
     1261 tgcgcatcga cggtacaact ggggtagtag accagtacaa cctggtcgcc aggcgaagaa
     1321 gttcagacaa tgtgcccgat gtcggtattc gtattgaaaa tctcggcggc ggagttgcaa
     1381 atattccttt tcagaacggt atccttcccg ttgatccttc cgggcatggc accgtcaata
     1441 tgcgcgcctg gccagttaat ctggtcggtg gtgagctgga aacaggaaaa tttcagggca
     1501 cagccaccat taccgtcatg gtgcggtaaa agaagacgtt gcgttttgat taacgcaacg
     1561 cttcggcact gttacccctg ctgctccagc gcatacttat acagcgcatt tttcttcacg
     1621 ccatgaattt ctgcggccag cgccgccgct tttttcagcg gcagttctgc ctgtagcagc
     1681 gccagcgtgc gcagggcatc ggcgggtaag tcttcttcct gtgctttatg accttcgaca
     1741 atcagcacca tttcgccttt gcgacggttt tcatcttcct ttacccacgc cagcagctcg
     1801 ccaacgggcg cgccgtgaat ggtttcccag gttttggtca gctcacgcgc cagaaccacg
     1861 tagcgggatt cgcctaatac cgcaacgata tcttccaggc tatctaacag acggtgggta
     1921 gattcataaa aaatcagcgt gcgcggctcc gcttcaatgg cttttagcgc atcacggcgg
     1981 ccttttgatt tggcaggtaa aaagccttcg taacagaaac ggtcagatgg taaacccgcg
     2041 gcgcttaacg cagtgatagc agcacacggt ccgggtagcg gcaccacacg aatccccgct
     2101 tcacggcagg tacgcaccag atggtagcca ggatcgttaa ttagcggcgt tccggcatcg
     2161 gaaaccagcg caatgttttg cccctcttgc agctttgcca gcagcgtttc ggctttttgt
     2221 tgttcgttat ggtcgtgcag cgcaaacaac cgggcattaa tcccaaaatg ttgcagcaat
     2281 aaaccggtgt gacgagtatc ctcggcggca atcagatcaa cggcctgtaa tacctctaac
     2341 gtacgctggg tgatatccgc cagattgccg attggcgtcg gtacaatgta aagctggccc
     2401 tgagaattat ccgccgattg gtgttgtttc attgtgtcgt ccgtattgcc gatttaatat
     2461 tgagcattgc gtaaaaaaaa tatcactgga tacattatgg taccctcaac attttctcgt
     2521 ttgaaagccg cgcgttgtct gcctgttgtt ctggcagccc tgattttcgc cggttgtggc
     2581 acccatactc ccgatcagtc cactgcttat atgcagggca cggcgcaggc tgattctgcc
     2641 ttttatcttc agcagatgca gcaaagctct gatgatacca ggatcaactg gcaattactc
     2701 gccattcgtg cactggtgaa agaaggtaaa accgggcagg cggttgagtt gtttaaccaa
     2761 ctaccgcaag aactgaacga ttctcagcgt cgcgagaaaa cactgctggc ggcagagatt
     2821 aaactggcgc agaaagattt tgctggcgcg caaaacttgc tggcgaaaat cacacctgcc
     2881 gatttagaac aaaaccagca agcgcgttac tggcaggcaa aaatcgatgc cagccagggg
     2941 cgtccttcca ttgatttact gcgcgcgtta attgctcagg aaccgctgct tggcgcgaaa
     3001 gaaaaacagc agaatattga tgccacctgg caggcgctct cctccatgac tcaggaacag
     3061 gcgaatacgc tggtgatcaa cgccgacgaa aatattctgc aaggctggct ggatctgcag
     3121 cgcgtctggt ttgataaccg taacgatccc gacatgatga aagccgggat cgccgactgg
     3181 cagaaacgtt atccgaacaa tccgggcgcg aaaatgctgc caacgcagtt ggttaacgta
     3241 aaagcgttta aaccagcctc gaccaacaaa atcgccctgc tgttgccgct gaatggccag
     3301 gcagcggtat ttggtcgcac tattcagcaa ggctttgaag ccgcgaaaaa tatcggcact
     3361 cagccagtgg cggctcaggt agctgccgca cctgccgcag acgtagcaga acaacctcag
     3421 ccgcaaactg cggatagcgt tgccagcccg gcacaagcct cggttagcga tctgaccggt
     3481 gatcagcctg cggcccagcc ggtgcctgta agcgccccgg cgacaagcac cgcagcggta
     3541 agcgcacccg caaatccatc cgcagagctg aaaatctacg atacctcatc acaaccactt
     3601 agccagatct taagccaggt tcagcaggat ggcgcgagta ttgtggttgg tccgttgctg
     3661 aaaaataacg ttgaagagtt gctgaagagc aacaccccgc tgaacgtact ggcactgaac
     3721 cagccggaga atatcgaaaa ccgcgtcaat atttgttact tcgcgctttc accggaagac
     3781 gaagcgcgcg atgcagcgcg tcatattcgt gaccagggta aacaagcgcc gctggtgctg
     3841 atcccacgca gtgcattggg cgatcgcgta gccaatgcgt ttgcgcaaga gtggcagaaa
     3901 ctgggcggcg gcaccgttct gcaacaaaaa tttggttcca ccagcgaatt acgcgcgggt
     3961 gttaacggcg gttctggtat tgctttaacg ggtagcccga ttactcccag agcgacaacc
     4021 gactccggca tgacgaccaa caatccaacg ctgcaaacca cgccaaccga tgaccagttc
     4081 accaataatg gcggtcgtgt cgatgcggtc tacattgtgg caacgccggg tgaaatcgct
     4141 tttatcaaac cgatgatcgc catgcgtaac ggtagccaga gcggtgcaac gctgtacgcc
     4201 agttcccgca gcgcacaagg caccgcaggc ccggatttcc gactggagat ggaaggcttg
     4261 cagtacagcg aaatcccgat gctggcaggc ggtaatctgc cgttaatgca gcaggcgctc
     4321 agcgcggtga acaacgatta ttcgctggct cgcatgtatg cgatgggcgt tgatgcctgg
     4381 tcgttggcaa atcatttctc acaaatgcgc caggttcagg gttttgaaat caacggtaat
     4441 accggaagtc tgacggctaa cccggattgc gtgattaaca ggaagttatc atggctacag
     4501 taccaacaag gtcaggtagt ccccgccagt taaccaccaa acagaccggc gatgcgtggg
     4561 aagcacaagc gcgtcgctgg ctggaaggca aaggactgcg gtttatcgcc gctaacgtga
     4621 acgagcgtgg cggcgagatc gatctgataa tgcgtgaagg cctgaccacc gtttttgtcg
     4681 aggtacgcta tcgccgctct gcgctttatg gcggcgcggc agccagtgtg acccgcagca
     4741 aacaacacaa attattacag actgcccgct tgtggctcgc gcgtcataat gggagttttg
     4801 atactgtgga ttgccggttc gatgtggtag ccttcaccgg gaatgaggtt gagtggatta
     4861 aggatgcctt taatgaccac tcataattaa ggtttaagga ttagcgtgca agaaagaatt
     4921 aaagcttgct tcactgaaag cattcaaact caaattgcgg cggcagaggc gcttccggat
     4981 gccatctccc gtgcagccat gacgctggtt cagtctctgc tcaatggcaa caaaatcctc
     5041 tgttgtggta atggaacttc cgctgccaat gcacagcatt ttgctgccag catgatcaac
     5101 cgtttcgaaa cggagcggcc cagcttacct gccattgcac taaatactga taatgttgtc
     5161 ttaacggcga ttgccaacga tcgcttacat gatgaagtgt atgcaaaaca ggtgcgggcg
     5221 ctgggtcatg cgggagatgt attgttagcc atttccaccc gtggcaacag ccgcgatatt
     5281 gttaaagcag ttgaagccgc cgttacgcgt gatatgacca ttgtggcatt gaccggctat
     5341 gacggcggcg aacttgcagg tttgttaggg ccacaggatg tggagatccg cattccttcg
     5401 catcgtagtg ctcgcattca ggaaatgcat atgctgacgg taaattgcct gtgcgatctg
     5461 atcgataaca cgcttttccc tcaccaggat gattaaggag aatacatgaa ggcattatcg
     5521 ccaatcgcag tccttatttc cgcgctgctg ttgcaaggtt gtgttgccgc tgccgtagtg
     5581 ggtaccgctg ctgtgggtac caaagccgca actgacccac gcagtgtcgg cacccaggtg
     5641 gacgatggta ccctggaagt gcgcgtgaac agcgcattgt cgaaagacga acagattaag
     5701 aaagaaaccc gtattaatgt cacggcctat cagggcaaag tgctgctggt tgggcagtca
     5761 ccaaatgctg aactttcggc tcgcgccaaa cagattgcta tgggcgtaga cggtgccaac
     5821 gaagtgtata acgagattcg tcagggccag ccgattggtc tgggcgaagc atctaacgat
     5881 acgtggatca ccaccaaagt gcgttcgcag ctcttaacca gcgacctggt gaaatcgtcc
     5941 aacgtgaaag tgaccaccga aaacggtgaa gtgttcctga tggggctggt gactgaacgt
     6001 gaagcgaaag cggcggcaga tattgccagc cgggtgagcg gcgtaaagcg ggttaccaca
     6061 gcgtttacgt ttattaaata gcagcccctt gtaatgcctg atgcgacgct tgccgcgtct
     6121 tatcaggcct acaacgatgc aaaccgtagg tcggataagg cgtttacgcc gcatccgaca
     6181 tgttttccct caaatcagaa caacagcgcc agcccgccga caatcacacc agacactgcc
     6241 accatcgccc ctgtcagcca taaggctttc gccgggaacg ctttgcgcag cataatcagt
     6301 gacggcaaac tcactgccgg gagcgtcatc aacaatgcca gcgccggagc ggttcccata
     6361 cctgccagca tcatcgtttg tacaatcgga atttctgctg ccgtgggaat gacaaacaag
     6421 catcctgcta ccgccatcgc caccacccac atcaggctgt tatcgacagc accatcggca
     6481 tgggggaata accagacgcg tgcggcaccc aacaccagta ctgcaaggat gtaaaccggg
     6541 atcgtactcc agaaaagtgt ccatagcgcc ctgccccagc ggctaaaaaa accgctctgt
     6601 gcttccggta tgtcaatttc gaccggtgcc tgcgtttgcg gtgtttcacg cacccatttt
     6661 tgcaccagcg tcgcaatcag caacaccatc accagcccgg ccaccagacg aatcgccgca
     6721 aaaccccagc cgaggacaaa ccccataaac accagcgtcg ccgggtttaa caccggattg
     6781 cccatccaga atgccagcgc accgcccatc gatacctgtt ggcgacgcat tcccgccgcg
     6841 accggagccg cacagcaggt acacatcatg cccggcagcg aaaacagcgt tcccagcagc
     6901 gtgccgcgaa agcgcgattg cccaagcgta cgcaacaacc agtcacgcgg gatcagcacc
     6961 tgaatcaacg aaccgaggat cacccccagc accgccgctt tccatactgc gaggaaatag
     7021 atcatcgcgt aatccaacgc cgcttgccac ggattagcat ccgcctgcgc aaggatagat
     7081 ttaccgatac tgtgggtttc ggctgcagta aacgctttgc cgtagtaagg ttgccatttc
     7141 acataccaga gaccggcaat gacaacgaga aagaaaagcg cgggtttcca ccactgaatg
     7201 ggcgttgccg cctgagatga agactgacca gtcatagcat tccccaggaa taagttatga
     7261 taattgagcg cgtgaatatt acgctcacta tcaattcttg ggaataatta ttcagctctt
     7321 ttgcgtaatt ctgaagagct taaaatcgtc acgccttcat gctcaggccg catcgcctct
     7381 gccagcatga cccgcgccac gtctctcgca tcaatggatt tccagttacc tggtaacaaa
     7441 cggaacagcg gcgcaaaaag cgtttcgttc atccgctgtt tgctacgatc gcccagtaac
     7501 atcgacgggc gagcaatggt caatttcggc cagttctggg caattaatgc ttcttccatc
     7561 tcccctttga cgcggttata gaaaaacggc gagtgggcat tggcacccat agcactgacc
     7621 accagcatat gctgcgcgcc cagtcgccgc ccggttaatg cggtatccac taccagcgtg
     7681 taatcggcat gaataaacgc ctctttgctc cccgcttctc gccgcgtggt gccgagacaa
     7741 caaaacacaa tgtcgatggg ttcggtgacc tgcgccaacg cgtcactcag ttgcggatca
     7801 tggggattaa acaccccggg catatcgccc aacggacgtc gcgtcggcgc agcaatggcg
     7861 ttaactttcg gttcattaat caacatccgc agcaggtgac cgcccaccag ccccgttgcg
     7921 cctgtaatca gtacctgact catcttcgtt cctttacaga attgtctgcc ttgcgctccc
     7981 cggctcatgc accagtctta atagaccatg aggtaattat ccccgattgt ggaaaattcg
     8041 cctcatccaa tgcaacaacg cggaggaagc atgagtaaga aaattgccgt tttaatcact
     8101 gatgaatttg aagattcaga atttacttca cccgcagacg agttccgtaa agccggacac
     8161 gaagtgatta ctattgaaaa acaagcgggt aaaacggtga aaggcaaaaa aggagaagcc
     8221 agcgtgacca tcgataaatc catcgatgaa gtgacgccag cggagtttga tgccctgttg
     8281 ctaccgggtg gtcattcacc ggattatctg cgtggcgaca accgttttgt cacctttacc
     8341 cgtgattttg tgaatagcgg caaacctgtt ttcgccatct gtcacggtcc gcagctgctg
     8401 atcagcgccg atgtgattcg ggggcgcaaa ctgaccgcag ttaaaccgat cattattgat
     8461 gttaaaaatg cgggcgtgga attttacgat caggaagtcg tggttgataa agatcagctg
     8521 gttaccagcc ggacaccgga cgatctgcca gcgtttaacc gcgaggcgtt acgcctgctc
     8581 ggtgcctgag tcgcgtagcc aaatcatttt tttaccaaag cccagcgtgt tgtcggtgaa
     8641 tttgatttca tcgagccgga tttcccacac cggggccgac agcattctgg caaccggaaa
     8701 gcgacgattg tacgccttgc gcgcgaggtc gctttcctca ccttccagcc tgcggatctc
     8761 acctttaaac tgcacaccgc gaattaacgc taccgttttc ggctgaccgt ttaccgttcc
     8821 ggcaaccgca gcctgcggcc cactcatctg cgcgtggcgc gttttttctt ccgtcaaaat
     8881 gtagaaggcg actttctgcg catcaaaaag ataaaaggca ttagcgcacc agagttcccc
     8941 ttcctgctgc acacaccagg tgacaacatg ttgttttgcc agccaacggc tgatggcgat
     9001 gagtgtttcc attgctgttc tcttttatac tgtgggcctg accttaacat gcgatgcaga
     9061 cgatgacacc ctggtttctt tacttgatcc gtaccgccga caataagctt tataccggga
     9121 tcaccacgga tgtcgaacgc cgctatcagc agcaccaaag cggcaaaggg gcgaaagcac
     9181 tgcgcgggaa aggagaactg acgctggcgt tttctgcgcc agtgggcgac cgttcgctgg
     9241 cactacgggc ggaatatcgc gttaagcaac tgaccaaacg gcaaaaagag cgtctggtag
     9301 cggaaggcgc agggtttgca gaactgttga gcagcctgca aaccccggag attaaaagcg
     9361 attgaaatgc tcgtgatact caaccagacc ggttacgcca ttcagcgcgt catccgctaa
     9421 acgatgtacc aggaaggcgc tttcagtgcc cggccagcgg caacgcagat cgtgatgcgc
     9481 cgccagttca aagccgaaac gactgtacag cgccggatcg cccagcgtca ccactgcggc
     9541 atagccgaac tcattaagcg aatcgagtcc ttcatagacc agttggcgtg ccagcccctg
     9601 tccacggtat ttttcatcaa ccgccaacgg tgccatgccg acccattgca ggtcttcgcc
     9661 ctgcacatca accggactaa atgccacata gccaatgacc tgaccttcgt catctgtcgc
     9721 caccagcccc agcgtcagaa agccatcttc acgcagatcg tgaaccagct tcgcttccgc
     9781 atcactttcg aatgagcgac gcagcagggc atcaataccc ggcgcatcaa tgggaatttc
     9841 tactcgaatt agcatggttc acctaccgat gtctgtttgg tttcaggcgc ggttttcatt
     9901 cccgcctcaa caaaatccgc cagttgcagc agcatcatgc gtaaggcttt cggcatttgc
     9961 tccagttcaa tggcgtccat caggtttttc acatacaccc ccagctccgt atcgccttca
    10021 atcaccagcc gacgctggaa gaagagcgta tccggatctt gtttacgcgc cgcaatcatc
    10081 agcagatcgc tggcgtcggc actaaaactc acatcagctt gcgtgttctg gctgaccacc
    10141 agtttgccat tcaccaccga ggtaaaccat tgcaggtcaa tatcacgcac atgaatactt
    10201 aaccagcggc cttcaagaaa ctccagctcg ccatcatcca gcgcctgacg gaattgccag
    10261 cttaagacct gctcaagaac ctggcgtttt agcgcaaatg gcgtcagttt taccggtaca
    10321 ctcaacagag atggccccaa atgcacaata cgggaacgca gtttatccaa cacgagtttt
    10381 actccctgtt tcaacaatca tcctattttg ccatatcaga aaaataacat agcggtataa
    10441 atcaacaatt ccatatgaaa ttgctgctac caccaataca actttaactg ccttaaatca
    10501 aaaattgtcg cagcaaggtt aactaaaatc ccagttcgtt aacatttttg cgttttgata
    10561 gcgcaacctt caggaaaaat tatggagctg ctctgccctg ccggaaatct cccggcgctt
    10621 aaggcggcca tcgaaaacgg cgcagatgct gtttatatcg ggctaaaaga tgataccaat
    10681 gcccgtcact tcgccggcct taactttacc gagaaaaaat tgcaggaagc ggtgagtttt
    10741 gtccatcaac atcgccgcaa acttcacatc gcgattaaca cttttgcgca tccggacggt
    10801 tacgcccgtt ggcagcgtgc cgtggatatg gcggcgcagc tgggtgccga cgcgctgatc
    10861 ctcgccgacc tcgccatgct ggagtacgcc gccgagcgtt acccgcatat tgaacgtcat
    10921 gtgtcagtgc aggcttcggc gaccaatgaa gaggcgatta acttttatca tcgccatttt
    10981 gacgttgccc gcgtggtgct gccgcgcgtg ttgtcgattc atcaggtgaa acagctggca
    11041 cgggttactc ctgtcccgct ggaagtgttt gctttcggca gcctgtgtat tatgtcggaa
    11101 ggccgttgct atctgtcgtc gtatctgacg ggtgagtcgc ccaacactgt gggtgcgtgt
    11161 tctccggccc gtttcgtgcg ctggcaacaa acgccgcagg ggctggaatc ccgcctgaac
    11221 gaagtgctga tcgaccgtta tcaggacggc gaaaacgcag gttatccgac gctgtgtaaa
    11281 gggcgttatc tggtggacgg cgagcgctat cacgcgctgg aagaaccaac cagtctcaat
    11341 accctggaac tgctgccgga gttaatggct gcgaatattg cttcggtgaa aattgaaggc
    11401 cgccagcgta gcccggcgta tgtcagccag gcggcgaaag tctggcgtca ggctatcgac
    11461 cgttgtaagg ccgatccgca aaacttcgta ccgcaaagcg cgtggatgga gacgctcggg
    11521 tcgatgtccg aaggcacgca aaccaccctt ggcgcgtatc accgtaaatg gcagtgagaa
    11581 gagcaatgaa atattcctta gggccagtgc tgtggtactg gccaaaagag acgctggaag
    11641 atttttatca gcaagccgcc gccagcagcg ccgacgtgat ttatcttggt gaagcggtat
    11701 gcagcaagcg ccgggcaacc aaagttggcg actgtctgga gatggcaaaa tcgctcgccg
    11761 gaagtggcaa gcagattgtg ctctccacgc tggcgctggt gcaggcatcc tctgaactgg
    11821 gcgaactgaa acgctatgtt gagaacggtg aatttctgat tgaagccagc gatctcggcg
    11881 tggtgaatat gtgcgctgaa cgcaaactgc cgttcgtcgc cgggcacgcg ctgaactgct
    11941 acaacgcggt gacgctgaaa atattgctca aacagggcat gatgcgctgg tgtatgccgg
    12001 tggagctttc ccgcgactgg ctggtgaatc tgcttaatca gtgcgatgag ctgggcattc
    12061 gcaaccagtt tgaagtggaa gtcctgagct acggtcatct accgctggcc tactccgccc
    12121 gctgctttac cgcgcgttcg gaagaccgcc cgaaagatga gtgtgaaacc tgctgcatta
    12181 agtatccgaa cgggcgcaac gtgctgtcgc aggaaaacca acaagtgttt gtactcaatg
    12241 gcattcagac catgagcggc tacgtttaca acctcggtaa cgagctggca tccatgcagg
    12301 gattggttga tgtagtccgc ctgtcaccgc agggcactga cactttcgcg atgctcgacg
    12361 ccttccgcgc taatgaaaat ggcgctgtgc cactgccgtt gacggcaaac agcgactgta
    12421 acggctactg gcggcggctg gcaggactgg agctgcaagc ctaagtaaat agctcacttt
    12481 gttaacaact ttaactattc tttaatgcag tattaaagat taatcggtaa caaagtgagc
    12541 tgttatgact gataaaacca ttgcgttttc gctactcgat ctggccccca tacccgaagg
    12601 ctcttcagcg cgggaagcgt tctcccactc tctcgatctc gcccgtctgg ctgaaaagcg
    12661 cggctatcat cgctactggc tggcagaaca ccacaatatg accggcattg ccagtgctgc
    12721 aacatcggta ttgattggct atctggcggc gaataccacc acgctgcatc tggggtctgg
    12781 cggcgtgatg ttgcctaacc acttaccgtt ggtcattgcc gaacagttcg gcacactcaa
    12841 tacgctctat ccggggcgaa tcgatttggg gctgggtcgt gctccgggta gtgaccaacg
    12901 gacaatgatg gcgctacgtc gtcatatgag cggcgatatt gataatttcc cccgcgatgt
    12961 cgcggagctg gtggactggt ttgacgcccg cgatcccaat ccgcatgtgc gcccggtacc
    13021 aggctatggc gagaaaatcc ccgtgtggtt gttaggctcc agcctttaca gtgcacaact
    13081 ggcggcgcag cttggtctgc catttgcgtt tgcctcacac ttcgcgccgg atatgttgtt
    13141 ccaggcgctg catctttatc gcagtaactt caaaccgtcg gctcggctgg aaaaaccata
    13201 cgcgatggtg tgcatcaata ttatcgccgc cgacagcaac cgcgacgctg aatttctgtt
    13261 tacctcaatg cagcaagcct ttgtgaagct gcgccgtggc gaaaccgggc aactgccgcc
    13321 gccgattcaa aatatggatc agttctggtc accgtctgag cagtatgacg tgcagcaggc
    13381 gctgagtatg tcgttggtag gcgataaagc gaaagtgcgt catggtttgc agtcgatcct
    13441 gcgcgaaacc gacgctgatg agattatggt taacgggcag attttcgacc accaggcgcg
    13501 cctgcattcg tttgagctgg cgatggatgt taaggaagag ttgttgggat agtgtgtctt
    13561 aacgcgggaa gccttatccg agctggcaac gctgtcctac gtaggcctga taagcgaagc
    13621 gcatcaggca ttgtgtaggc agcagaaatg tcggataagg caccgctgat tactgataca
    13681 ccggcagtaa attaaagctc gataaaatat gcaccagtgc gttgccgacg ccaaacacca
    13741 gaatcagcgc aatcatcggc ttgccacccc agacgcggaa tttcgggctg ccaaagcgtt
    13801 tacgcgatgc gcgggccagc agtgccggaa caattgccgc ccagatggta gccgctaaac
    13861 cagcataacc aatggcatac aggaatccgt tcgggaacag cagaccaccc acaacaggcg
    13921 gggcaaaggt cagcaatgcc gttttcaagc ggcccacagc cgagtcgtcg aaaccaaaca
    13981 gatctgccag atagtcaaac aaacccagcg ttacgccaag gaacgaactc gctaccgcaa
    14041 agtttgagaa cacgaccagc agaaggtcca ggctacggct gttcagcacg ccgcttaatg
    14101 cttgtaccaa cacatcaata ttaccgccct tctcggcgat gccgataaac tccggacgag
    14161 ggatgttgcc cattgtcgcc agcaaccaga tggtatacag cgccagtgcc atcagcgtac
    14221 cgtacaccag gcatttcacg atggttttcg gatctttgcc gtaatacttc atcaggcttg
    14281 gcacgttacc gtgataacca aacgacgcca ggcagaacgg cagggtcatc agcaaatacg
    14341 gcgcataaga tgcattgctt tcggcaacgt tgaacaatgt cgcaggctgc acatgcccca
    14401 gcaggctacc gaaggtgagg aagaaggtaa tgactttcgc ccccagcaca atcgccgtca
    14461 tgcggctgac ggctttagta ctcaaccaca ccacaaacgc taccagcagt gcaaaaccaa
    14521 aacccgccgc ccgtgccggg acgtttagcg acatctctgc gaaggtgtga tgcagaatcg
    14581 aaccactggc agaaatatag gcataggtca ggatatagag cacaaaggca atggaaatgc
    14641 cgttgaccac gttccagcct ttgcccagca aatctttggt gatggtgtca aaactcgaac
    14701 cgattcggta attcaggtta gcttccagaa tcatcaagcc ggaatgcagc atacagaacc
    14761 aggtaaagat cagcgctgcc attgaccaga aaaaccacgc cccggacatg accactggca
    14821 gagaaaacat ccctgcgcca ataatggtgc cgccgataat caccacgccg ccaagcagcg
    14881 acggtgacgt ttgggtggtg gttagtgttg ccatgagggc ttctctccag tgaaaaatag
    14941 tgcgactgcg ttgttatgca ttgcactgta ccagtacatg agtacaaaag acagaaaaaa
    15001 agccccgatg gttaaaatcg gggctgtata tattatttta cagattgtgt tcgctgttca
    15061 gcgatgatta cgcatcacca ccgaaacgac gacgaccggt agagtcatca cgacgcggtg
    15121 cgcggccttc acgacgttcg ccgctaaaac gacgaccatc accacgacca ccttcacggc
    15181 gttcaccgct gaagttacga ccgccttcac gacgctcgcc accgaaacca cgaccaccgc
    15241 cacgacgctc accgccagta tgcggctgtg catcgcccag taactgcatg ttcatcggct
    15301 tgttgagaat gcgagtgcgc gtaaagtgtt gcagcacttc acccggcata cctttcggca
    15361 gttcgatggt ggagtgagaa gcaaacagct tgatgttacc gatgtaacgg ctgctgatgt
    15421 cgccttcgtt agcaatcgcg ccaacgatat gacgaacttc aacaccatca tcgcggccca
    15481 cttcaatgcg gtacagctgc atatcgccaa catcacgacg ttcacgacgc ggacgatctt
    15541 cacggtcgcc acgcgggcca cggtcgttac gatcgcgcgg accacggtca tcacggtcac
    15601 ggaattcacg tttcggacgc atcggcgcat ctggcggaac aatcagagta cgttcaccct
    15661 gtgccatttt cagcagtgcc gcagccagag tttcgagatc cagctcttca ccttctgcag
    15721 tcggctgaat tttgctcagc agtgcgcggt attgatccag atcgctgctt tccagctgct
    15781 gctgtacttt agcggcgaat ttttccagac ggcgtttgcc cagcagttct gcgttcggca
    15841 gttctacttc cggaatagtc agcttcatag tacgttcaat gttgcgcagc agacgacgct
    15901 cgcggttctc aacgaacagc agcgcgcggc cagcacgacc cgcacgaccg gtacgaccga
    15961 tacggtgaac gtaagactca gaatccatcg ggatatcgta gttaaccacc aggctgatac
    16021 gctcaacgtc caggccacgg gccgcaacgt cggtcgcaat caggatgtcc agacgaccgt
    16081 ctttaaggcg ttccagtgtc tgctcacgca gcgcctggtt catgtcaccg ttcagcgcgg
    16141 cgctattgta gccgttacgc tcaagagctt cagccacttc cagagtcgcg tttttggtac
    16201 gaacgaagat aatcgccgca tcaaaatctt ccgcttccag gaaacgtacc agtgcttcgt
    16261 ttttacgcat accccacaca gtccagtagc tctggctgat gtcaggacgg gtagtcacgc
    16321 tggactgaat acgcacttcc tgcggctctt tcataaagcg gcgggtaatg cgacgaatcg
    16381 cttccggcat ggttgcagag aacagagcgg tctgatgacc ttccgggatc tgcgccataa
    16441 tggtttcaac gtcttcgatg aagcccatgc gcagcatttc gtcagcttca tccagaacca
    16501 gaccgctcag tttagagagg tccagagtgc cacgtttcag gtggtccagc agacggcccg
    16561 gagtaccgac aacgatctgc ggcccctgac gcagggcgcg taattgcacg tcataacgct
    16621 ggccgccgta cagggcaacc acatttacgc cgcgcatgtg tttagagaaa tccgtcattg
    16681 cttcagcaac ctgtaccgcc agttcgcggg tcggtgccag caccagaatc tgtggtgctt
    16741 tcagctcagg atcaagattc tgcagcagag gtaaagagaa tgctgcagtt tttccgctcc
    16801 ccgtctgggc catacccaga acgtcgcggc cattcagcag atgtggaata cactctgcct
    16861 gaattggaga tggtttttcg taacccagat cgttaagggc ttcaaggata ggagccttca
    16921 ggcccagatc tgcaaaagtg gtttcgaatt cagccatgta gtacgtgtgc ctcaaaatta
    16981 atggcggcca gtctacataa ctcatcatga aattgatcag caattttcat tgaaaagtgt
    17041 gaaccggctc aaagtaggtg tattaacgaa caacaacgcc ctcacccgtt aaggtgatgg
    17101 caatcaaaaa agattacggg ctgatgtgta cgtcagctat tgctggtccg attctgccag
    17161 gtcatcttgg tcctggccca ggagcgataa ttccaacaat gcgtatcggt gctcaacaaa
    17221 gttatgaacg ttgttggcaa ccgccagttt gaacagtgcc gtggcgctgt ccaaatcccc
    17281 cagacttagg tagtacttac ctaaatagaa gttggtttca ctgagatgct cagcgagcga
    17341 ggtgttatcc gttgcgtccg ccttgagcct ttccattaac gtttgttcgc taatgttgcc
    17401 caggtagaac tcgacaatgt tccatcccca ctgttcctta tccgattttt cgaagtgctg
    17461 tttcaatact tctttagcct gcttctcatc gagcttctgc tcggcgagat aaagccacag
    17521 actacggaaa ggatcattgg gatcgtcttg ataaaacgcc agcagatcat cttgcgctaa
    17581 cttgtcacga ccgccgtaat ataatgcaat cccgcgattc aagtgcgcgt agttgtaagt
    17641 tggatcaagc tcaagtacag aatcaaacgc ttcataggca gcatcaaaat tgcctgcctg
    17701 cgttaaatat atgcctaagt aattgaatac ttcaggcata tccggtcgga ttgccagcgc
    17761 ttgcgaaaaa tcgttacgcg ctaatgccct cagaccgaga ctatcataca acactccgcg
    17821 ctcatataaa agctgtgcgc gttcgtcatc ggttaaagcc cgactggcaa ggatttgttc
    17881 catacgtgcc agaatcactt cctgctgtaa agtcggttgc aatggtaccg cgaggacttc
    17941 acttttacgc caggaagtat tactgcatcc tgcaagcgta agtgctgtcg caacgaaaca
    18001 ccagcgcaaa aaaggcttca tttcccactc ccgaagacca cggttgaatg aacgtcctgt
    18061 tcccggttgc taacaaggcg tcctgcccgg ttaaaagccc cccgccgcag cggagggcaa
    18121 atggcaacct tactcgccct gttcagcagc cggagcttcc ggtgctgcag caggttgaga
    18181 ctgctcagtc gcttctttaa tgctcagacg gatacggccc tggcgatcaa cttccagaac
    18241 tttcaccggt acttcctgac ccatctgcag gtaatcggtc actttctcaa cgcgtttgtc
    18301 agcgatttga gagatgtgta ccagaccttc tttaccgccg ccgatggcaa caaatgcgcc
    18361 aaagtcaacg atacgggtca ctttaccagt gtagacgcgg cccacttcga tttctgcagt
    18421 gatctcttcg atacgacgaa tagcatgttt cgctttctcg ccgtcggtcg ctgcaatctt
    18481 cacagtaccg tcatcttcga tttcgatggt ggtgccagtt tcttcggtca gggcacggat
    18541 aacagagccg cctttaccga taacgtcttt gatcttgtcc gggttgatct tgatggtatg
    18601 gatacgtggt gcgaactcag agatatcgcc acgcggcgcg ttgatcgcct gttccattac
    18661 gcccaggata tgcagacgcg cacctttagc ctggttcagc gcaacctgca tgatctcttt
    18721 ggtgatacct tcaattttga tatccatctg caatgcagag ataccctcgc gggaacccgc
    18781 aactttgaag tccatatcgc ccaggtgatc ttcgtcgccc aaaatgtcag acagtacaac
    18841 gtagttgtcg ccttctttca ccagacccat tgcgataccc gcaacggcag ctttgatcgg
    18901 cacacctgcg tccatcagcg ccagagacgc gccgcacacg gaagccatag aagaggaacc
    18961 gttggattca gtgatttcag acacaacacg tacggtgtac gggaatttgt ccatatccgg
    19021 catgactgcc agcacgccgc gcttcgccag acgaccgtga ccaatttcac gacgcttcgg
    19081 agaaccgacc atgccggttt cgcctacgga gtacggaggg aagttgtagt ggaacaggaa
    19141 ggtatcggta cgttcgccca tcagttcatc aagaacctgc gcgtcacgag cagtacccag
    19201 cgttgcggta accagcgcct gcgtttcacc acgggtgaac agcgcagaac cgtgagtacg
    19261 cggcagcacg ccagtacgca catccagacc acggatcata tctttttcac gaccgtcgat
    19321 acgcggttcg cctgccagta cgcggctacg aacaacgttt ttctcgatcg cgtgcagaat
    19381 ttcacccagt tcgttttcgt ccagggtttc gtcttcagca agcagcgtcg cgatggtttc
    19441 agatttgatg acatcaacct gcgcataacg ctcttgtttg tcggtgatgc ggtaagcatc
    19501 gctcaggcga gcttcagcca gtgcagcaac gcgcgcgttc agcgcttcgt ttaccggctc
    19561 cggctgccag tcccaacgtg gtttaccggc ttctttcacc agttcattga tgttctgaat
    19621 aacaacctgc tgttgttcat gaccgaacac tactgcgccc agcatctggt cttcgctcag
    19681 cagttcagct tcagattcaa ccatcagtac agcggcttca gtaccggcaa caaccagatc
    19741 cagtttgctc tctttcaact cgtcctgagt cgggttcagt acgtactggt cattgatgta
    19801 acctacgcga gcagcaccaa tcggaccatt gaacggaata ccagacagag acagtgctgc
    19861 ggaagcacca atcatcgcga cgatatccgg gttaacttgc gggttaacag aaaccacggt
    19921 ggcgataacc tgaacttcgt tgacgaagcc ttccgggaac agcggccgaa tcgggcggtc
    19981 aatcagacgc gcgatcaggg tttcgccttc gcttgggcgg ccttcacgac ggaagaagct
    20041 acccgggata cgaccagcag cgtaggtacg ctcctgatag ttaacggtca gtgggaagaa
    20101 gtcctgacct ggtttggctt ttttctggcc aacaacggta acaaataccg cggtgtcatc
    20161 catgctaacc ataacagcgg cagtagcctg acgagccatc atgccggttt ccagagtcac
    20221 ggtgtgttgg ccgtactgga atttacgaac gatcggatta agcaatgtaa tatcctttct
    20281 ctttcttaga cagtacctta cggcactggt gttaataccc gatcttctgc gcatcctcgc
    20341 gactaatgac aaccctaacc cagctctatg tgggtaaagc ctctcattag ccgcgcgaac
    20401 ctctgcaacg gaagatcatt catagcaaca atacattagt ttccagtgaa ttgctgccgt
    20461 cagcttgaaa aaaggggcca ctcaggcccc cttttctgaa actcgcaaga attagcgacg
    20521 cagacccagg cgctcgatga gctgggtgta acgtgctacg tctttacgtt tcaggtagtc
    20581 gagcagttta cgacgctgag aaaccatgcg cagcagacca cgacggctgt ggtgatcttt
    20641 tttgtgctct gcaaagtggc cctgcaggtg gttgatctgt gcagtcagca gtgctacctg
    20701 aacttcagta gaaccggtgt cgtttgcgtc acgaccaaac tcagaaacga ttttagctgt
    20761 tgcttcagta cttagagaca ttttaaaact ccaaagtata tagaatgaaa ggacgccgat
    20821 ctctaattca gcgatcccag tgtacgttac gcaaagtgtt aaacaattta cgcgacgtta
    20881 agcggcagta ttctactcgt agcgacctgt tatcgcaaga cggttaacat tacgccgggt
    20941 attcaaccac caggcgacga ggcgcaacgc ggccttcatc gtcaatttcg cccataccga
    21001 taaatttgcc gttctcacct tctgtaacgc gaaccagtcc ttccagcggc gcaccggatg
    21061 tacgaactgg gttaccattt ttgaagtaaa cagaagacgt taacggaaga ttcaccaccg
    21121 ggtagtccga agctggactg tccattggca tcagtaatgg atcaagtaac tccgcagccg
    21181 gaatatcctg ctgttcggct tgttcgacca gttcacgcag atgttccagg gtcacaatcc
    21241 gttcaaccgg atatttactt accgccagac gacgcaggta aataacatgc gcgccacaac
    21301 cgagtttttc acccaggtca tcaatgatgg tgcggatata agtgcctttt gagcagtgaa
    21361 tttccagctc cagctcattg ccttcgtggc gaataaacag caattcataa acggtaatcg
    21421 ggcgcgcttc acgcggtact tcaatgccct gacgcgcata ttcgtacagt tttttaccct
    21481 gatatttcag tgctgaatac atcgaaggga tctgttcgat atcgccacgg aaagtatcca
    21541 gcgccgccgc cagttgctct gcgctaaagg ttaccggacg ttcttcaacg atttgtccgt
    21601 cggcatcaga agtatcggta cgctgtccaa gacgcgcaat gacccgatag cgtttgtcgg
    21661 agtccagcag atactgggaa aacttcgtcg cttccccgag gcaaattggc aacatgccag
    21721 ttgccagcgg gtccagcgca ccggtatgcc cggcacggtt ggcgttataa atccgtttca
    21781 ctttttgcag cgcatcattg ctggacatac cctgcggttt atccagcaac aaaacgccgt
    21841 taatgtcgcg accgcgacga cgaggacgac tcattagtcc tccttgctgt cgtccgggtt
    21901 aacacgacgt tcttcgtcat gtttgaccac gctggtcacc aggtttgaca tgcgcatccc
    21961 ttcaaccaga gagttgtcgt agaagaaggt cagttccggc acgatacgca ggcgcatcgc
    22021 tttccccagc aggctgcgga tgaaaccaga agcttcttgc aacgctttga tgcccgcttt
    22081 aaccgcgtct tcatctttgt cgttgaggaa cgtcacatat actttggcat acgccaggtc
    22141 gcgagacatt tcgacaccgg aaacggtggt catcatgccc aggcgaggat ctttaatttc
    22201 acgctgcagg atgagagcga tctctttttg catttcctgc gctacgcgct gcgggcgacc
    22261 aaattctttc gccataataa attctcctga caaaaaaggg gctgttagcc cctttttaaa
    22321 attaatttca ggtggaaggg ctgttcacgt tggcctgata agacgcgcca gcgtcacatc
    22381 aggcaatcca tgccggatgc ggcgtaaacg ccttatcccg catggaaccc taaaaacctt
    22441 aagcgatggt acgttggatc tcgatgattt cgaatacttc gatcacatcg ccagtgcgga
    22501 cgtcgttgta gttcttaacg ccgataccac attccatacc gttacggact tcgttaacgt
    22561 catctttgaa gcggcgcagg gactccagct cgccttcgta gataaccacg ttgtcacgca
    22621 gaacgcggat cgggttgtga cgtttaacca caccttcggt aaccatacag cctgcgatgg
    22681 caccaaattt cggcgatttg aacacgtcac gaacttccgc cagaccgata atctgctgtt
    22741 tcagttccgg agacagcata ccgctcatcg ccgctttcac ttcgtcaatc aggttataga
    22801 tgacggagta gtaacgcaga tccaggcttt ccgcttcaat cactttacgt gcagaggcat
    22861 cagcacgtac gttgaagcca accaggatgg cgttggacgc cgcagccagg gtggcgtcgg
    22921 tttcggtgat accacctacg ccagaaccga tgatcttcac tttaacttcg tcagtagaca
    22981 gtttcagcaa ggagtcggag atcgcttcca cagaaccctg tacgtctgcc ttcaggacga
    23041 tgttcacttc gtgaacttcg ccttcggtca tgttggcgaa catgttctcg agtttagatt
    23101 tctgctgacg cgccagttta acttcgcgga atttaccctg acgatacagt gcaacttcac
    23161 gcgctttctt ctcgtcacgt acaacggtaa cttcatcacc cgcagccggt acgccggaca
    23221 ggccgaggat ttccaccgga atggacggac ccgcttccag cacttcctga cccagttcgt
    23281 tacgcatcgc acgaacacga ccgtattcga agccacacag aacgatatcg cccttgtgca
    23341 gagtaccttc acgtaccaga acggtagcaa ccggaccacg acctttatcg aggaaggatt
    23401 cgataaccgc accgctcgcc atacctttac gtaccgcttt cagctccaga acttccgcct
    23461 gcagcaggat agcgtccagc agttcatcga taccagtacc cgctttcgca gacacatgta
    23521 cgaactggct ttcaccgccc cactcttccg gcaggatgcc gtactgggag agttcgttct
    23581 taacgcgatc cggatcggct tctggtttat cgatcttgtt cactgcaacg accaccggaa
    23641 cctgcgccgc tttcgcgtgc tggattgctt cgatcgtctg cggcattacg ccgtcgtcgg
    23701 cagcaacaac caggactacg atgtccgttg cctgcgcacc acgagcacgc attgaagtaa
    23761 acgcggcgtg ccccggggtg tccaggaagg tgatcatgcc gttttcagtt tcaacgtggt
    23821 atgcaccaat gtgctgggta atgccgcccg cttcgccaga ggccactttc gttgaacgaa
    23881 tgtagtccag cagagaggtt ttaccgtggt caacgtgacc catgatggtc acaaccggcg
    23941 cgcgcggttc agccgcagca cccgtgtcac ggtcgctcat taccgcctct tccagctcgt
    24001 tttcacgacg caggataact ttatggccca tctcttcagc aaccagctgt gcggtttcct
    24061 gatcgataac ctggttgatg gttgccattg cgcccagttt catcatcgct ttgatgacct
    24121 gagagccttt aaccgccatc ttgttcgcca gttcgccaac ggtgatagtt tcgccgatca
    24181 caacgtcacg gttaacggcc tgagcaggct tctggaagcc ttgctgcagc gaagaacctt
    24241 tacgttttcc gcctttaccg ccacgtactg ctgcgcgtgc ttcttcacga tcagcttttg
    24301 attcagcgtg tttgttgcct ttcttcggac gcgctgcttt cgcgttacga ccacggccac
    24361 ggccgccttc gacttcacga tcgctttcgt cttctgcctg gcgagcatgt tgagaagtag
    24421 tgacgtgata atcgctggaa tcttcagtcg gttccgcgtt atcagtccat ttgttttctt
    24481 ccgccatacg acgtgcttct tcagcaacgc gacgtgcttc ttcttcgagt ttacgacgcg
    24541 cttcttcttc agctttacgc ttgagctctg cagcttcctg ctcacggcgg gctttttcag
    24601 cctgggcgtt tttagtcata tcgtcttgtt gattgctcac tttgtctttt tccgcagctt
    24661 cacgtttcgc ttgttcagca gcttcacgct tagcttgttc tgcggcctca cgttcagctt
    24721 tttgttgcgc ctcgcgttta gccgattctt ctgcctcacg acgggcttgc tcttccgctg
    24781 caaggcgttc agcctcttgc ggatcgcgtt tcacaaaggt gcgtttcttg cggacttcga
    24841 tttgtaccga tttgcttttt ccaccggtac caggaatgtt aagggtgctg cgtgttttac
    24901 gttgcagcgt caatttgtcc gggcctgaat ttttctgatt caggtggtca atcaaagtct
    24961 gtttctcttg tgcagacaca gagtcgtcag cagacttccg gatacctgca tcagcaaatt
    25021 gctgtaccag gcgttccacg gaggtctgtc gctctgcggc cagcgtttta atcgttacat
    25081 ctgtcatgct gttccttcct gctacagttt attacgcttc gtcaccgaac cagcaaatat
    25141 tacgggcagc cataatcagt gctccggctt tttcgtcggt caacccttcg atatcagcca
    25201 gatcatcaat gccctgttcg gcgagatctt ccagcgtaca aacgccacgg gcggccagtt
    25261 tgaatgccaa atcacgatct accccttcaa ggttcagcag atcgtcagcc ggtttgttat
    25321 caccgaggct ttcttcctgg gcctgtgcaa tggtggccag tgcattttta gcacgctcgc
    25381 gcagtgcttc aacggtcggc tcatcaaggc cttcgatttc caacagctct ttcatcggca
    25441 cataggccag ttcttccagc gtcgagaaac cttcttctac cagaacagtc gcgaagtctt
    25501 cgtcgatgtc gagatatttg gtgaaggtgt cgatcgctgc gtgcgcttcc gcctgatgct
    25561 tagcctgcag gtcgtcaacg gtcattacgt tgagttccca accgctcagc tgcgaagcca
    25621 gacgcacgtt ctgaccgtta cggccaatcg cctgcgccag gttaccagct tcaacggcga
    25681 tatccatggt gtgtttatct tcatccacca cgatagaagc aacgtctgcc ggtgccattg
    25741 cgttaatcac aaactgcgcc gggttatcat cccacaggac gatatcgata cgctcgccgc
    25801 ccagttcagc agaaaccgcc tgaacacgcg cgccacgcat acctacgcaa gcacctaccg
    25861 gatcgatacg tttatcgttg gttttcaccg cgattttcgc acgagaaccc gggtcgcgag
    25921 ccgctgcttt aatttcaatc acttcttcgc cgatttccgg cacttcaata cggaacagtt
    25981 cgatcagcat ttccggcttg gaacgagtga cgaacagttg cgcgccacgc gcttccgggc
    26041 gaacggaata aagcacgcca cgaacgcggt cgccagggcg gaagttttca cgcggcagca
    26101 tatcttcgcg caggatcacg gcttcagcgt tgttgcccag atccagagag atgttgtcgc
    26161 ggtttacttt tttcaccacg ccggtgatga tttcaccttc gtgttcacgg aactgatcaa
    26221 ccaccatcgc acgttcggct tcacgcactt tctgcacgat aacctgtttt gccgtctggg
    26281 tggtgatacg gtcaaaggta acagactcaa tctgatcttc aacgtaatcg cccaagttca
    26341 ggctttcatc ttcataacgt gcggcttcaa gggtgatttc cttggtcggc tgggtgactt
    26401 catcaacaac taaccagcga cggaaggtgt caaaatcacc gcttttgcga tcgatctgta
    26461 cacggacgtc gatctcttgc tcatattttt tctttgttgc tgtcgccagc gcgctttcca
    26521 atgcttcgaa aatcttctcg cgaggtagcg ccttttcatt ggatacggct tcaactacag
    26581 ccaaaatttc tttgttcatc gcgggctttt cacctcatcc agactattaa aagtggggaa
    26641 ccaggttcgc cttctggata ttactcagcg cgaacacttc atctttacct tcgacggtaa
    26701 ctgtgatcat ttcaccgtct accgctttga taacgccctg ccatttacga cggttttgta
    26761 ccgccatacg gagaaccaga gtcacctctt ctccgacaaa acgggcgtag tgttcagccg
    26821 tgaacagtgg gcgatcgaga cccggtgagg agacttccag gttataagca acggtgatgg
    26881 gatcttcaac atccagcaca gcacttacct ggtggctcac atcagcacaa tcatcaacat
    26941 tgatgccatc ttcactatca atatagatgc gcagtgtgga tgtgcgaccg cgaataaatt
    27001 cgatgcctac cagttcaaaa cccagggcct caactggcgc agtaatcatc tctgttaatt
    27061 tttgctctaa tgtggacaag cccaccccca agacataaaa aaagggccta aagcccagtt
    27121 attctgtagt cagataacaa aaaaccccga taaatcgggg ctttatataa ctgaacccta
    27181 taaccgcaac tgcggtctgg agcactttcc agaaggattt tttcaaatcc cactacgaag
    27241 gccgaagtct tcacagtata tttgaaaaag gactctaagg gaaagtggtt gcgggggccg
    27301 gatttgaacc gacgaccttc gggttatgag cccgacgagc taccaggctg ctccaccccg
    27361 cgcctgaaac gtggcaaatt ctactcgttt tgggtaaaaa atgcaaatac tgctgggatt
    27421 tggtgtaccg agacgggacg taaaatctgc aggcattata gtgatccatg ccacattttg
    27481 tcaacgttta ttgctaatca cgtgaatgaa tatccagttc actttcattt gttgaatact
    27541 tttgccttcc cctgctctcc cttaagcgca ttattttaca aaaaacacac taaactcttc
    27601 ctgtctccga taaaagatga ttaaatgaaa actcatttat tttgcataaa aattcagtga
    27661 gagcggaaat ccaggctcat catcagttaa ttaagcaggg tgttatttta tgacgacgat
    27721 tctcaagcat ctcccggtag gtcaacgtat tggtatcgct ttttccggcg gtctggacac
    27781 cagtgccgca ctgctgtgga tgcgacaaaa gggagcggtt ccttatgcat atactgcaaa
    27841 cctgggccag ccagacgaag aggattatga tgcgatccct cgtcgtgcca tggaatacgg
    27901 cgcggagaac gcacgtctga tcgactgccg caaacaactg gtggccgaag gtattgccgc
    27961 tattcagtgt ggcgcatttc ataacaccac cggcggcctg acctatttca acacgacgcc
    28021 gctgggccgc gccgtgactg gtaccatgct ggttgctgcg atgaaagaag atggcgtgaa
    28081 tatctggggt gacggtagca cctataaagg aaacgatatc gaacgtttct atcgttatgg
    28141 tctgctgacc aatgctgaac tgcagattta caaaccgtgg cttgatactg actttattga
    28201 tgaactgggc ggccgtcatg agatgtctga atttatgatt gcctgcggtt tcgactacaa
    28261 aatgtctgtc gaaaaagcct actccacaga ctccaacatg cttggtgcaa cgcatgaagc
    28321 gaaggatctg gaatacctca actccagcgt caaaatcgtc aacccgatta tgggcgtgaa
    28381 attctgggat gagagcgtga agatcccggc agaagaagtc acagtacgct ttgagcaagg
    28441 tcatccggtg gcgctgaacg gtaaaacctt tagcgacgac gtagaaatga tgctggaagc
    28501 taaccgcatc ggcggtcgtc acggcctggg catgagcgac cagattgaga accgtatcat
    28561 cgaagcgaaa agccgtggta tttacgaagc tccggggatg gcactgctgc acattgcgta
    28621 tgaacgtctg ttgaccggta ttcacaacga agacaccatt gagcagtatc acgcgcatgg
    28681 tcgtcagttg ggccgtctgc tgtaccaggg gcgttggttt gactcccagg cgctgatgct
    28741 gcgtgactct ctgcaacgct gggttgccag ccagatcact ggtgaagtta ccctggagct
    28801 gcgccgtggg aacgattatt caatcctgaa taccgtctca gagaacctga cctacaagcc
    28861 agagcgtctg acgatggaaa aaggcgactc ggtgttctcg ccagatgatc gtattggtca
    28921 attgaccatg cgtaatctgg atatcactga tacccgagag aaactttttg gttatgccaa
    28981 aactggcctg ctttcctcct ctgccacttc aggcctgccg caggtggaga atatggaaaa
    29041 caaaggccag taattcgaca tcaaccctgc ccttcagggg tggggttacc ttgcttatcc
    29101 tctgtaaact cctttctgag catcacataa tcgaaaacat catggtttgc atccacaacc
    29161 cgtgagtctc ctttatagtg tgcaatcact tcctcaagcg tttgcggctg ttccggcttt
    29221 gttaccccca tccacgcatt aataaggtaa ttattgtaag ctgtcgaaaa gatattgttt
    29281 tccgttgtgc gatccacgcc atccccaaga acggggctat accagataaa catcgggaca
    29341 tgatatgcct gctggctagc ctccctgcct ccatgaaaat agacgttctt cttcgtaggg
    29401 tcacgttcca ggccgtggtc ggcaaaatac atgaccgagg cgcggcgatc ttttaataat
    29461 tcaaaaacct gacctagcaa actatctgtg taatgaatgg agttgtcata gcaggcatcc
    29521 tgatcgtcct gcggttgaaa cacggcgctg gattgcggat aggcgctaca agccggttca
    29581 tggcttccat ttaaatgaag aacaatcagt ttcttttgct gcgtattttg ctgtaatgct
    29641 tgcgataaat gcggcaacaa caattcatca aatcctctga cataaactgt ttccatggcg
    29701 cgcatggcga tactggtaac tgctgtacca ttctgccgaa aagcggattg cgagcttagc
    29761 cagaaagtct gaaatcctgc ttgattagcc atattaataa tgttgtcggg gtaattatga
    29821 atgtcatgac tcaaaacaga atcagcagtt aaagaaaggg gaaccgacag cgcagtgtaa
    29881 ggtgcgccgc ttattgcttg attaaacagt ttgatctgtt ttctttgtgc ttcaacttgc
    29941 ggtgtcgtag agcgtgtata tccatacaaa gacatattgt cgacacgtac agactccccc
    30001 acaatcaaca cgtaggtatc aatacttgta tccctgactg ataattgaaa atacggcacc
    30061 gtgtttgcaa ttgagagtaa tctttgatgc gctttcgctg ctaaagcaaa atagttgaga
    30121 ttgaaaaacg gcgtataggt agcaaatcac gacgctagta tatatggact gaacgctttt
    30181 ttattttttg catctttata agcaaattga caagcggaaa ataaactgcc cgaaatgaca
    30241 atcagcaata atattcctgt cacttttttt gtcggcaagg aaacatcata ttttattatt
    30301 actgccaaaa aaagaaggga taaaaaagca aaagcacata gataagggat atacatcccc
    30361 agcattttca caacttcatc cggatcactc tgcagcacac taatcgcaaa tccatcattg
    30421 aaagttgtac caaaggtaca ccatgaatag aggctaatac tcatgtctgc cgcagtcagt
    30481 aacacaaatg gtgcggcaac aagcaggcga agcggaaggg atgatatacg tttaacaacc
    30541 aggatggtca ggacgaagaa cagcaatcgc tgaaccatat gcgctccgga agaggcgact
    30601 aagttcgtaa taccaaaaac aataacacaa agatacaaca accaatgaga tttaaaatct
    30661 tctagcaaat ttgttgaata ctgtcatcca gaaatccaag ttcacaacca atataaacaa
    30721 ttatcaacaa tgttcaattt tatatcagac atgtttttca actctgatcc atatcagacc
    30781 gaaacactgt tagttttttc tttttaaatg atgcgaccat tgcgttctca gtaattaaat
    30841 aacattatca atgcgtctct aaacattacg cagaacgtag ccatcagtag ttatactgac
    30901 aggagcagga tggtttatcg agaagtaaac atatccgtat tcgtaccgga actggagttg
    30961 acacaataaa gtgccaatta tgtcagcagt agggaaaagt ttataactaa ggcgtaccgg
    31021 caccatcgtt tcaaggtacc agctacgagt aaagcaactg gacgagatac agatacctga
    31081 caaccattca tcagaccagg accaaaacga aaagagacgc ttttcagcgt ctcttttctg
    31141 gaatttggta ccgaggacgg gacttgaacc cgtaagccct attgggcact accacctcaa
    31201 ggtagcgtgt ctaccaattc caccacctcg gcacggatac tactttttag ttcgggatat
    31261 cgctggtcgg cttagccgga gcagctggct gagtttgttc ggttttcgcc ggtgcactca
    31321 gattttccca ttcgctacct ctattggttt tgttgctatt gatgttaccc agcaccagac
    31381 tgatgatgaa gaataacgtt gccagcagcg ccgtcatgcg ggtcatgaag ttaccagaac
    31441 cacttgaacc aaacagcgta gcggaagcgc ctgctccgaa ggaggctccc atatcagcgc
    31501 ctttaccttg ctgcagcatg atcagaccaa caaggccaat tgccacaata aggaaaacta
    31561 ctaaaagagc ttcgtacata atcaacctgt tccttgcgga gttgccgcgt accaatgctt
    31621 caaccaataa agcgggagtt tttaatcttt cccactgaag cgggtgtgaa tactaaccaa
    31681 aacgaatgac cttcgcaagg gcaattttat cgcattgtat caactgcgga aaaaaacagc
    31741 aaaaccgatg tgttcgccga gaaaaaaggc gacaatcgcc gcctttttag ccagttatct
    31801 aacgctttaa acggctttta ctgcatcggc gatgcggtgt gcaaattcag tcacctgcgc
    31861 ttcgtcttcg ccttccacca tcacgcgaat taacggttcg gtgccggatt tacgcagcaa
    31921 cacgcgtcca cggttgccca gcgcagcttc aacctctgcg gtcacggctt taactgactc
    31981 atgctcaagc ggatcgccgc tacctgcggt gtaacgtacg ttaaccagaa tctgcgggaa
    32041 cattttcatg ccgctgcaaa ggtcgtgcag gctcatatgg ttacgtgcca tcgccgccag
    32101 cacctgcaag ccagcaacga tgccgtcacc ggtagtggtt ttatccagca ggatcacatg
    32161 accggaattc tctgcaccga tacgccagcc tttctcctgc attttttcca gtacgtagcg
    32221 gtcacccact ttcgcgcgcg caaatggaat tcctaactgt ttcagcgcca gttcaagccc
    32281 catgttgctc atcaatgtac ccacagcacc accacgcagc tggccctgac gaagaccttc
    32341 acgcgcgatg atatacatga tctgatcgcc atcgacttta ttgccttcat ggtcaaccat
    32401 aatcacgcga tcgccgtcgc cgtcgaaggc aataccgaga tccgcttttt cagccagcac
    32461 acgagcctgg agcgcgcgaa cgtcggtagc ccccacttcg gcattgatgt ttacaccgtt
    32521 tggctcacaa ccgatagcga taacgttcgc ccccagttcg cgcagcacgt tcggcgcgat
    32581 gtgataagtc gcaccgtttg cacaatccac cacaatcttc agttcactga ggctaagttc
    32641 gttcgggaac gtggctttgc aaaactcgat atagcgaccc gcggcatcaa cgatacggct
    32701 ggctttaccc agttcagccg aatcaacgca gctgatctcc ttttccattt ccgcttcgat
    32761 ggcctcttct accgcatccg gcagtttagt gccgtcgata gagaagaatt taatcccatt
    32821 atcgtagaac gggttatgcg atgcagatat cacaattccg gcctctgcgc ggaaggtacg
    32881 cgtcagataa gccacggccg gtgttggcat cgggccagtg aagagtgcgg aaaggcccgc
    32941 tgccgccaga cccgcttcca gtgctgactc cagcatatag ccagaaatac gcgtgtcttt
    33001 accaataata atcttacggg agccgtggcg cgccagcact ttacccgcgg cccaacccag
    33061 cttaagcaca aaatcaggtg tgatcggcgc atcccctaca cgaccacgaa tcccatcggt
    33121 accgaaatat ttacgattac tcatagcgtt tgttttcctt tgcagacaga gtggcttcca
    33181 ccacccgcat cgcttctacg gtttctttga cgtcatgaac acgaatgatg tgcgcgcctt
    33241 gcattgcggc aatgaccgca caggccagac taccgctcag gcgctcggac ggccccacgt
    33301 tcagcagctg cccaatcatc gattttcgtg acatacccac caacagcggc aggttgaaat
    33361 ggtgaaattc agccaggcgc gccagtaatg aatagttatg ggagagattt ttaccgaaac
    33421 cgaatccggg gtcgagcaac aatttctctt ttgcgatacc cgcctgctcg caacgtgcta
    33481 tttgctcaat aaagtagcga ttcacttctg caaagacatc gtcatacttc ggagcttcct
    33541 gcatggtttt tggatttccc tgcatatgca tcagacaaac cggtaaaccg gtttctgcag
    33601 ccgcctccag agcgccaggt tcggaaaggg agcggatatc attaataatg tgagcgccaa
    33661 ctttcgctga ctcacggatg acttctggtt tggatgtatc gaccgagatc cagacttcga
    33721 agcgttgagc aattgcctca accacaggaa taacacgttg caactcttct tcaacgctaa
    33781 cttccgccgc ccctgggcgc gtggactcgc caccaacgtc aatgatcgtc gcgccagcat
    33841 tgatcatcag attcgcatgt ttcaccgcat ctatcagcga gttatgcgtg ccaccatccg
    33901 aaaaggaatc aggcgtgacg ttgaggatcc ccattacgtg aggatggcta aggtccagtg
    33961 aagtaccctg ggcaaagagt ttcattatgt tatccctggt atgaattgat aagaaaaaac
    34021 cccggagcac gccccggggt tttcggtaca aatacagtca tctgatgcgg gaacttactt
    34081 gtcgcctaac tgctctgaca tggtgttacc cgggttcggc gtacgcggtt catcaaccgg
    34141 acgaggagcc tttggactac cattgtcgcc agaattgtta gaagcgcctg gttcttccca
    34201 gcccgctggc ggacgtacat cgcgacgtgc catcaggtca tcaatctgcg gtgcgtcgat
    34261 agtctcatat ttcatgagag catctttcat cgcatgcaga atatccatat tgtcggtcag
    34321 aagctgacgc gcacgattat agttacgctc aatcagtgct ttcacttcct ggtcgatgat
    34381 acgtgcagtt tcatcggaca tatgtttcgc tttcgctacg ctacggccga ggaatacttc
    34441 accttcttct tccgcgtaca gcagtggacc caatttctca gagaagcccc actgagtcac
    34501 catgttacgt gccaggttgg tcgcaacttt aatatcgttg gacgcaccgg tagaaacatg
    34561 ttccggcccg tagatgatct cttctgccag acgaccaccg tacagcgtag aaatctggct
    34621 ttccagtttc tgacggctgg cgctgattgc gtcgccctca ggcaagaaga aagtcacacc
    34681 cagcgcacga ccgcgtggga taatcgtcac tttatgcacc ggatcgtgtt ccggcaccag
    34741 gcgaccgata atcgcatgac ccgcttcgtg gtaagccgtt gattctttct gcgcttccgt
    34801 catcaccatg gagcgacgtt ccgcacccat catgattttg tctttcgctt tctcgaactc
    34861 aaccatcgac acaacgcgtt tgttgccacg agcagcgaac agtgccgctt cgttcaccaa
    34921 gttcgccagg tcagcaccgg agaaaccagg agtaccacga gcaatgattg ccgcgtcgat
    34981 atcgggtgcc aatggtacgc gacgcatgtg aactttcagg atctgctcac gaccgcgaac
    35041 atctggcaag ccgaccacaa cctggcggtc gaaacggcca ggacgcagca gggccgggtc
    35101 gagaacgtcc ggacggttag tcgcggcgat aacgatgata ccttcgttac cttcgaagcc
    35161 atccatctca accagcatct ggttcagagt ctgttcacgt tcatcgtgac caccgcccag
    35221 accagcgcca cgctggcggc ctacggcgtc gatttcatcg ataaagatga tgcacggtgc
    35281 cgctttcttc gcctgttcga acatgtcacg aacacgggat gcacccacac cgacgaacat
    35341 ttctacgaag tcagaaccgg agatagtaaa gaacggaact ttcgcttcgc ctgcaatcgc
    35401 tttcgccagc aacgttttac cggtacccgg aggaccgacc atcaggacgc ctttcgggat
    35461 cttaccgccg agtttctgga agcggctcgg ctcgcgcaga tactctacca gttcagcaac
    35521 ttcttctttt gcttcgtcgc agcccgcaac gtcagcaaag gtcgttttga tctgatcttc
    35581 cgtcagcatg cgcgctttgc tcttaccaaa cgacatggca cctttgccac cgccgccctg
    35641 catttgacgc atgaagaaga tccagacacc aatcagcaac agcatcggga accaggagat
    35701 gaagatagaa gccagcaggc ttggttcttc aggcggttca ccgacaacct tgacgttctt
    35761 ggtcaacagg ttatccagta atttcggatc ctgaaccgga atgtaagtgg tataacggtt
    35821 actatctttc ttggtaacgt tgatttcacg tccgttgata cgcgcttcac gaacctggtc
    35881 gttattgacc tcttgtagga aggtagagta atccacctta cggccattag actcgctggg
    35941 cccaaagctc tggaatactg acatcagcac aacggcaatg accagccaga gtattaggtt
    36001 tttcgccatg tcactcaagg gattaacctc ttattacaac tgtgttaaaa acagcgtcag
    36061 gatactctat atccagcatc tttcaaactt tcgtctgaaa tctcccggtt agggtttacg
    36121 cccggtcgct acaatataca cttctcgcga acgtgcacga gaagagtccg gcttacgaac
    36181 tttgaccttc gtaaacaggg agcgaatttc ccttagatac tcatcgaaac cttcgccctg
    36241 gaacaccttc actacaaaac tgccacctgg cgctaataca tcacgacaca tttctagcgc
    36301 cagttccacc agatacatgg ctcgggggat atccaccgcc ggtgttccgc tcatgtttgg
    36361 tgccatatcg gacatgacaa cctggacttt gctgtcgcca acgcgctcca gcagtgcttt
    36421 catcaccagt tcatcacgaa aatcgccctg aagaaagtcc acaccaacga taggatccat
    36481 aggtaaaaga tcgcaagcga tgatgcggcc tttgccgcca atttgggtga ccacatattg
    36541 tgaccaacca cccggagcag caccaaggtc gacaaccgtc attcccggtt taaagagttt
    36601 gtcactttgc tgtatttcat caagtttaaa ccaggcacgg gaacgtaatc cctttttctg
    36661 tgcctgttga acatatttat cgctaaagtg ttcctgaagc cagcggctgg agctggcaga
    36721 acgcttctta cctgtcattt aactttccca tggggataac tcatcgtaac caattgcgta
    36781 aatttttacg cgcctatttg gtgatatatg ggagatggcg gtagaatgac ccgttttcaa
    36841 tcccaacgta agcaaaaaat acgatgaatc tgagtactaa acaaaaacag cacctgaaag
    36901 gtctggcaca tccgctcaag ccagttgttc tgcttggcag taatggtttg accgaagggg
    36961 tgctggccga gattgaacaa gcgttagagc accatgaact catcaaggtg aaaatcgcca
    37021 ccgaagatcg cgaaacaaaa accttgatcg tggaagctat cgtgcgcgaa accggcgcct
    37081 gtaatgtaca ggtcatcggt aaaacgctgg tgctttatcg cccaactaaa gaacgtaaaa
    37141 tctcgctgcc acgctaagat tatcctaaag ttacacacat ttgctgtgta aaacgagggg
    37201 ttttccgcag gcaggagagc aaaatgccac gctctgttcg ttgataaaag gccgcagagc
    37261 ggcctttttc ctttctttac aatacatcaa catcttgagt attgggtaat tcttacaggt
    37321 attccacctt aattacttca aattctactt cgccgcccgg cgttttgatg accacaacat
    37381 catcttcttc tttgccgatc aggccacgag caataggcga gtttacagaa atcaggtttt
    37441 gtttaaagtc agcttcgtca tcgccaacga tgcgataagt ctgttcttcg tcagaatcca
    37501 gattcagcac cgttacggta gcaccaaaaa taacgcgccc attgttgggc attttggtga
    37561 catcaatcac ctgcgcgttt gacagcttgg cttcgatgtc tttgatacgg ccttcgcaga
    37621 agccctgctg ttcacgagct gcgtggtatt cggcgttttc tttcaggtcg ccatgctcac
    37681 gcgcttccgc gatagcagca atgatttcag gacggcgcac agatttcaga aaatccagct
    37741 cttcgcgtaa tttttcagcg ccgcgtaagg tcatcggaat agcttgcatt tgttatacct
    37801 cttgaatatt cctgatgggg caagtcttca cccaccccgg ctgttcggcc tgcccggcat
    37861 aactcctcac cgggaccaga agcaaaaaaa taccgacccg ggtacaagtc ccaggtcagc
    37921 tacaattcac attttgatgg tcattttacc ctgaagttcc cgaagggtca tcgtttactt
    37981 tatagggcgt tgcgccgtag tatgacggct cgattccagg ttgttagcgc gagattatgc
    38041 gattttccag atttatcatc ggattgacca gctgtatagc gttcagtgtt caggccgcga
    38101 atgttgatga gtacgttact caactccccg caggtgccaa ccttgccctg atggtgcaaa
    38161 aagtcggtgc gtcggccccc gctattgatt accacagtca gcagatggcg ctgcccgcca
    38221 gtacccagaa agtgattact gcgctggcgg cgttgattca actcggcccc gattttcgtt
    38281 tcaccacgac gcttgaaacc aaaggcaatg tggaaaacgg cgtacttaag ggtgacttag
    38341 tggcgcgatt tggtgccgat ccgacgttaa aacgtcagga tattcgcaat atggtcgcga
    38401 ctttgaaaaa atctggcgtc aaccaaatcg atggcaatgt gttgatagat acctccattt
    38461 tcgccagcca cgataaagcc cccggctggc catggaatga catgacacaa tgctttagcg
    38521 ctccgcctgc tgccgccata gttgaccgca actgtttctc cgtctcgctc tacagtgccc
    38581 caaagcctgg tgatatggct tttatacgcg tggcatctta ttaccccgtt acgatgttca
    38641 gccaggtacg caccctcccc cgtggttctg ccgaagcgca atactgcgaa ctggatgtgg
    38701 tgccaagcga cctgaaccgc tttacgctga cgggatgcct gccacaacgt tctgagccgc
    38761 tcccgttggc ttttgccgtg caggatggag ccagctatgc cggtgcaatt ctgaaagatg
    38821 agttaaaaca ggcgggtatc acctggagcg gaacactgct gcgccagact caggttaacg
    38881 aacctggaac ggtagttgcc agtaaacagt cggccccgct gcacgatctg cttaagatta
    38941 tgctgaaaaa gtcggacaac atgatcgccg atacggtttt ccgcatgatc ggccatgcgc
    39001 gcttcaatgt gcctggaaca tggcgggctg ggtcggacgc cgtgcgtcag atcctgcgcc
    39061 agcaagccgg tgtcgatatt ggtaacacca ttattgccga tggttcaggg ctttcgcggc
    39121 ataacctgat tgcccccgcc accatgatgc aggtgctgca atacattgcc caacacgaca
    39181 atgaacttaa ctttatctcc atgctgccgc ttgcgggcta tgacggctct ttgcagtacc
    39241 gtgcaggtct gcatcaggcg ggcgtggatg gcaaagtctc agcgaaaacc ggttcgttgc
    39301 agggggtata taacctggcg ggattcatta ccacagcgag cgggcaacga atggcatttg
    39361 tacaatatct ttctggctat gcagtaaaac ccgcggatca gcgtaatcgc cgtattccgt
    39421 tagtgcgttt tgaaagccgt ttgtacaaag atatttatca gaacaattag tcaaaaagaa
    39481 accccggcgc atggccgggg tttcagatta ttgacaaagt gcgctttgtt tatgccggat
    39541 gcggcgtgaa cgccttatcc ggcctacaaa atcgtgcaaa ttcaatatat tgcaatcatc
    39601 ttgtaggcct gataagtgta gcgcatcagg cttatttggc attggtcatt agtgattaac
    39661 gcttgtaaat gaactcaacg ccttcttcgt cgtcttcgtc ccagtcgtca tcccagtctt
    39721 catcatcctc ttcagcaatc tcttcaagct gctgacggtg ataatcatcc cacatgaatt
    39781 cgactttctc tggctgtttc gcttcttcag cctgcacgac cgggttttca atgataaagg
    39841 tcatcacatc ccagcagaga tctttcacgc ccagtccgct cgccgcagag atcagataat
    39901 atttatcttc ccagcccagc gcttcagcga tcgctttcgc tttctcttcg gcttctgcct
    39961 tatccagcag atcgatcttg ttgaagacta accaacgcgg tttcgccgcc agatcctggc
    40021 tgtatttttc cagctcgctg ataataatac gcgcgttttc aaccggatcg gtgccgtcaa
    40081 tcggatcgat atcgatgagg tgcaacagga cgcggcaacg ttccaggtgc ttcaggaagc
    40141 gaatgcccag acctgcgcct tccgcagcgc cttcaatcag tcctggaata tcggcaacaa
    40201 cgaagctctt ttcgttgtcc attcgtacca cacccagact tggcaccaga gtggtaaacg
    40261 gataatccgc cactttcggt ttagccgccg ataccgcacg aataaaggtc gatttacccg
    40321 cgtttggcat ccccaacata ccgacgtcag ccagcagcat cagctccagc agcagctcac
    40381 gcttatcgcc cggcgtaccg ttagttttct gccgtggtgt acggttaact gacgatttga
    40441 aacgggtatt gcccagaccg tgccagccgc ccttagcaac cagcagacgc tgaccgtgtt
    40501 tagtcatatc acccatggtt tcaccagtac cctggtcgat tacacgcgta cctaccggca
    40561 ctttaatcgt cacgtcttta ccgcgcttac cggtacaatc gcggcttgcg ccgttctgac
    40621 cgcgctctgc acggaaagat ttttcaaaac gataatcgat aagcgtgttc aggttctcgt
    40681 cggcttccat ccatacgtca ccaccatcac cgccgtcgcc gccatccggg ccgcctttcg
    40741 gaatatactt ttcgcggcgg aagctcacgc aaccattacc gccatcacct gcaacgacca
    40801 gaatcgatgc ttcatcaaca aacttcattt tattctccgt aaatcattcg cctgcgcggg
    40861 gttgcgatac caccgttgta tgcttacgta atccgcccca aatacgatga ccaatggcgg
    40921 aatacatcgc gcccgcaacc acgacaaacg caccgagata acctaaaagg tttaacatcg
    40981 gtctggcgaa gaaatcgggc caggccagtg ataaaagatc tgaaaaaaac agcgtaaaca
    41041 gtggggtgag cgtgatgatc gcgctcacct gcgctgcctg ccagcgagcc atcgcttccg
    41101 ccaaggcgcc atatcctacc aaggtattca gtccgcaaaa aattaaacat gcgagctgcc
    41161 agtggctaag ctgcgctatc actccaggct ttgccagagg gaagagcgcg attgtacata
    41221 aagtgtacag taaaaacagg atctgcggtg aggccagccg acgcaataaa accttttgcg
    41281 ccacgccata actcacccaa accgtcgccg caccgacccc aaagataact ccccaggtgt
    41341 aatcggtgag ctttgtaaat atctcgacca gactggtgtt aaaaaacatc accagaccgc
    41401 tcaggagcat caatgcccct acaacctgag tgctgcgcat tttctctttc aggataaata
    41461 cgctggcaac catcatgcca actggcgaga gttgcccaat cacctgcgaa gcggtcggac
    41521 tcaggtattg caaggatgag ctgaacagga tgaagttccc aaacagcccg gcggtcgcca
    41581 ctgccaacaa aatcaaccag cgtggcttac gaaacacgcg taatggcggc aacctcttct
    41641 tcaccgcaag aatggcacca aggccaatac tcgccatcaa gaaacggtaa aacacgattg
    41701 tcggaggttc catcacctcc agcacctgct tcattgcgat tggcaacgcc ccccagcaaa
    41761 ttgctgtggt aagcgccaaa agaatgccaa tgcctgcctg ctgcttcata ccgtattccc
    41821 tgcaaagcgc atttgcggat taacgtcgca ccatgcgcat ttttaccggt tatcgaatgt
    41881 aaaaagcccc gcaacgtgtt gcggggcttt catccgttac cgggacgcga aaaacttatt
    41941 cagcttcgat gctgataaat ttacggtttt tcgggccttt aacttcgaat ttcactttac
    42001 cgtctgcttt agcaaacaga gtgtggtcac gaccgcaacc tacgttagcg ccagcgtgga
    42061 atttggtacc acgttgacga acgatgatgc tacccgccag aacggattcg ccaccgaaac
    42121 gcttaacgcc caagcgttta gcttctgaat cgcgaccgtt acgtgtggag ccgccagcct
    42181 ttttatgtgc catttgaaat ctctcctcag gtcttaggcg ctgatgccag taattttcac
    42241 atcagtgaac cactgacgat ggccctgctg cttacgatag tgtttacgac gacgaaactt
    42301 aacgatttta actttctcgc cacgaccgtg agcaacaact tcagctttga ttacgccgcc
    42361 atcaacgaaa ggaacgccga ttttgacttc ttcaccgttt gcgatcatca gcacttcagc
    42421 gaactcaaca gtttcgccag ttgcgatgtc cagcttttcc aggcgaacgg tctgaccttc
    42481 gcttactcgg tgttgtttac caccactttg gaaaaccgcg tacatataaa actccgcttc
    42541 cgcgcacacc ttttcaatga ttcagagtgc gctataaata ttcacaatag ggcgcgaata
    42601 ttacgcaaaa cgcacgcctt tgacaagtgc tacagtcaat acacgaagaa aaaaacacaa
    42661 cttgtacggt aacgtttatc tgtgccattt tttcagtaca attaccctat attcctaacc
    42721 ataaacccta agttgccttt gttcacagta aggtaatcgg ggcgaaaagc ccggcttttg
    42781 cgatgaattt agaaaaaatc aatgagttaa ccgcgcaaga tatggcgggt gttaatgcgg
    42841 caatccttga gcagcttaat tccgacgtcc aactgatcaa tcagttaggc tattacatcg
    42901 tcagcggcgg cggtaaacgt attcgtccga tgattgctgt actggctgca cgagctgttg
    42961 gctatgaggg aaatgcgcat gtcaccattg ctgccctgat cgagtttatc cacacggcga
    43021 ctctgctaca cgacgacgtt gtggatgaat cagatatgcg caggggtaaa gctaccgcca
    43081 acgccgcatt tggcaatgcc gccagcgtgc tggtaggcga ttttatttat acccgcgcgt
    43141 tccagatgat gaccagcctc ggttcactca aagtgctgga agtcatgtca gaagctgtaa
    43201 acgtcatcgc agaaggtgaa gttctgcaac tgatgaacgt taacgatccg gacatcactg
    43261 aagaaaacta catgcgcgtt atctatagca aaaccgcgcg tctgtttgag gctgccgcgc
    43321 agtgttccgg gattctggct ggctgtacgc cggaggagga gaaaggcctg caggattatg
    43381 ggcgctatct cggcactgct ttccagttga tcgacgattt actcgattac aatgccgatg
    43441 gcgaacagtt aggtaaaaac gtcggcgacg atctgaacga aggtaaaccg acgctgccgc
    43501 tgctgcatgc gatgcatcat ggcacaccag aacaggcaca gatgatccgt accgccatcg
    43561 aacaaggtaa cggtcgccat cttctggaac cggttctgga agcaatgaac gcttgtggat
    43621 ctcttgaatg gacgcgtcag cgcgccgagg aagaagcaga caaagccatc gcagcgttac
    43681 aggtgctccc ggacacccct tggcgagaag cactcatcgg cctcgcgcac atcgctgttc
    43741 aacgcgatcg ttaatcccct cccttcatcc cgcgcagtgc gcgggatgat tccagtaaat
    43801 tccataaaaa cactaattca gcgctataac acctgaaaaa agggcgatga atctttatat
    43861 attctgaata ttcacactct ttacaggaac tttttagcgc aatagaccat caggagtata
    43921 gtgatgcccg acagaagaag tgttctgaat gaaagcgact aacttaagga gtgaggaaag
    43981 atggaaagta atttcattga ctggcatccc gctgacatca ttgcaggttt gcgcaagaaa
    44041 ggaacgtcaa tggcggcgga atctcgcaga aatggtttga gttcctcaac gctggcaaac
    44101 gcattatcgc gcccatggcc gaaaggagag atgattattg cgaaagccct gggaactgac
    44161 ccctgggtta tctggccatc acgctaccat gatccgcaga cccatgagtt tatcgacaga
    44221 acgcagttga tgcgtagcta cactaaaccg aaaaaatgag tggtctggcg gtagccccgc
    44281 gaacggggct gccagctctc agacgattac tcgcctttca cacgctcaat atttgcacct
    44341 aaagcgcgca gtttgtcttc aatgcgttcg tagccacgat cgatgtgata aatacgatca
    44401 accaccgtcg tcccttccgc aatacagcca gccagcacca ggcttgctga tgcacgcaga
    44461 tcggttgcca taacctgtgc accagaaagt ttttcaacac catgacaaat aacggtattg
    44521 ctttcgattt cggcgtgcgc gcccatacgg ctcagctctg gcacatgcat aaagcggttt
    44581 tcaaagaccg tttcggtgat gaacccggtc ccttcagcca ccaggttcaa cagcgtgaac
    44641 tgggcctgca tatcggtcgg gaatgccgga tgcggcgcgg tacgtacgtt aacagccttc
    44701 ggacgtttgc catgcatatc caggctaatc cagtcttcgc cgacttcgat gtccgctcca
    44761 gcatcacgca gtttcgccag cacggcgtcc agagtatctg gctgcgcgtt acggcagata
    44821 attttgccac gagagatcgc cgccgccacc aggaaagtac cggtttcgat acgatccggc
    44881 agcacgcgat agacaccgcc gcctaaacgt tccacacctt cgatgacgat acgatcggtg
    44941 ccctgaccgc taattttcgc acccagcgta atcaggaagt tcgcggtatc gacgatttcc
    45001 ggttcacgcg ctgcgttttc aataatcgtg gtgccttccg ccagggttgc agcacacatg
    45061 atggtcaccg ttgcgccaac gctgacttta tccatcacga tatgtgcgcc tttcagacga
    45121 ccatcgacgg aagctttaac gtaaccttct tccagtttga tggtcgcgcc taattgttcg
    45181 aggccagaaa tgtgtagatc aaccggacgc gcaccgatcg tacaaccgcc aggtagtgaa
    45241 acttgcccct gaccaaagcg cgctaccagc ggccccagcg cccagataga agcacgcatg
    45301 gttttaacca gatcgtaagg tgcgcagaat acattaacgt cgcgggcatc aatatgcaca
    45361 gaaccattac gttctacttt cgcacccagc tggcttagca gtttcattga tgtatcgacg
    45421 tctttcagtt tcgggacgtt ctggatctct accggttctt ccgccagtag tgcggcaaaa
    45481 agaataggca gagcagcatt tttagcgccg gaaattgtga cttcgccctg gagcttcgtt
    45541 ggcccctgaa cacgaaattt atccatttag tttgttctca gttaacaatt catatccgct
    45601 accggcgaat cgcccatagc tcaaaagccg ttcagtttgc gatcgcgcgc ccactccgca
    45661 ggggtatacg ctttgatcga cacagcatga atgcggttat ccgcaatata ttccatcagc
    45721 gggccataga ccgtctgctg ttttttaacc cgactcatac cgtcaaacaa ctcacccacg
    45781 gcaataacct gaaagtggct gccatcgccg gaaacgtgga cttcctggag ggagagagcg
    45841 ttcatcaaca cgctctgaat ttcattattt tccatgggat cttcaatcat cagttaataa
    45901 accagcgaaa catcttagag caaagttgcg ctggcataaa taagcaaaaa gcctcgctga
    45961 taaatcagac aaggctcgac ttgcaggcag gtttgccgga caggcggtta acgccatatc
    46021 cggcctgaaa aaatttaacg aggcagaaca tcagcaggca aattatacaa tttcgcgagg
    46081 gtatacactt tgtcgtttac cccctgaagc gtcacgttgt tgccctgctt tttcgccaga
    46141 tcgataagat ggagcagcag tgccagtccc cccgtatcca cgcgggacac acggctaaga
    46201 tcgatgcagg taatcccctt caccgcctcc tcacgcattt cccaaagcgg tagcagaacg
    46261 tcctgatcca gctctccgga taacgccagc gtgtcacccg tctgcatcca gctcagtgac
    46321 tcgctcatta ttttttctct tccagagtga ttttctgttg agaaatcgat ttcagttgcg
    46381 cagtcaggcc gtcgatacct ttggtacgca gcagcgttcc ccactcgttt tgtttggtgg
    46441 tgatcatact gacgccttca gcaatcatgt cgtaagcttg ccagtttccc gtctgggagt
    46501 ttttacgcca ctggaagtcc agacgcaccg gcggacggcc attcgggtca ataatggtaa
    46561 cgcgaatagg cacaatggtt ttatcgccca gcggctgttc tggcgcaatc tgataggttt
    46621 gaccgtgata catcgccagc gcctgaccgt aagcctgctt caggtactca cggaaagcgg
    46681 caaagtaggc ttcacgttga gcaggggtcg cactcttgta atactggccc agcaccagcg
    46741 caccggcgta tttcacctgt acgtatggca gcagttcctg atcaacaatg gtacgcagat
    46801 aatccgggtt ggcccgaatt tgcggttgct cattcttcag gcgatcgaac gttttctgcg
    46861 ccgcctcgtc catcagctta taaggattgg tctggtctgc cgcggttgcc gcactcagag
    46921 gtgcaatcac cagcaaagcg accatcatta aacgtttaaa catgcgtcgg ttctcctgaa
    46981 attatttcgt tgtacccaca ggttcagtgg tttcattatt acctggcgca gcagctggcg
    47041 catcgccact attcttattg tcatcgcctt tactaccgta aaggaactga ccaatgagat
    47101 cttccagcac catcgcagac ttggtgtcct gaattgtatc gccatccttc aggatagcag
    47161 tccccagttc cgggtcttca aaaccgacgt ttaatgccag atattgttct cccagcaggc
    47221 cggaagtacg aatgctcagc gaactggtat caggaatgtg gttataacgt tgttcaattt
    47281 ccagcgttac gcgcggcaga taggttttcg ggtccagcgt aatatccgcc acccggccca
    47341 caacaacacc accaatgctg accggagagc gcgctttcag gccgccaatg ttatcgaacg
    47401 tcgcataaag cgtgtaggtc gattcagtac gtatggacgt cacgttcgcc gccttcaggc
    47461 aaacaaacag cgccgccagc agtgctgcta ataaaaagat acccacccaa atttcatttt
    47521 ttttcgtttg catgaactca attcccaaac atcaatgcgg tcagcacaaa atccagcccc
    47581 agaacagcca gagacgagtg gacaacggtg cgagtggttg cccggctaat cccggcagac
    47641 gtcgggatgg cgtcgtagcc gttaaacaac gaaatccacg tcaccgtgat ggcgaacacc
    47701 acgctcttaa tcagacagtt gaccagatcc atacgccagt cgacggcatt ttgcattgcc
    47761 gaccagaaga acccgctatc aatgcctttc caactgacgc cgaccagcga tccgccccag
    47821 atccccacgg cgacgaagat aaccgtcaac agtggtaatg aaataacccc agcccagaaa
    47881 cggggagaaa taacccgacg cagcggatcc accgccatca tctccatact ggagagttgc
    47941 tctgtagcgc gcatcaggcc gatttctgcg gttaacgccg aaccagcacg cccggcaaac
    48001 aacagcgcgg caaccaccgg ccccagttca cgcagtagcg ataacgccac cagcataccc
    48061 agactggttt ccgcgctata agtggtcaga accagataac cttgcagccc caacaccatt
    48121 ccgatgaaca cgccagaaac tacaataatc agcatcgaca ggacgccgac attatagagc
    48181 tggcgcacca gcagcggcgc atgtttgcga aattccggtt tgccgaccag cgcattgaat
    48241 aacattaacc cggcccgccc gaacgttctc agggttttaa tccctttatg tccgagcgac
    48301 gccagcgcat ttaacagcat gagtggctta actccctggt aaaagatcag cgtgataatc
    48361 gccggcagga tagcggaacg gaacaggccc gtcagctatc ccgtccagaa actgacgtac
    48421 gcgcggatca ggattcgtct gcaacgcctg ggcactgcca tgagcgacaa tttttttgtc
    48481 cgccaggatc caggcgtgat ccgcaatact taacacttcc ggcacatcgt gagaaaccac
    48541 cacacaagtc acgcccagcg cgctgttcag ctcagaaatc agcttcacca gtacgcccat
    48601 ggtgatggga tcttgcccaa caaaaggttc atcaaacatg atgagatccg gctccagcgc
    48661 aatcgcacgc gccagcgctg cacgccgcgc catcccaccg gaaagttcag aaggcattag
    48721 tttagccgct ccacgcagcc ccacggcctc cagcttcatc atcaccgtac tatgcaacaa
    48781 tggcgcggga agttgggtat gttcgcgcag tggataggcg acgttgtcaa atacgttcat
    48841 atcagtgaac aacgccccgg actgaaataa catgctcatc cgtttacgca ctgtatacag
    48901 gcgcgaacga gacatcgccg gaatattctc accatcgaaa aggatctcac catgatctgg
    48961 tgcgatttgc ccgccaatca gacggagtag cgtcgtttta ccgatgcccg atggccccat
    49021 gatcgccgtg atcttccctc gcggcacggt cagggaaata ttatcgaaga tgcagcgatt
    49081 gccacgcgta aaactgacat cgcgcatatc gactaaattc gccacagact gctccataat
    49141 tcacccttcg tcttgcgttg attttctaag catggcgctc aatttaacct tgaacccaac
    49201 atatttacag aatattaccc gccgtggtta gcgaaagctg gcatttgttt tactttttag
    49261 ccgcataaag tcaaaattaa gaatccgtta cggctttctg aaaatcttca gcggaccggc
    49321 gagtatacct gaagaaagga cgttagatgc ttttagctac ggcactgtta attgttggtt
    49381 tacttttggt cgtttacagt gccgaccgcc tggtttttgc cgcgtctatt ctttgccgaa
    49441 cctttggcat cccgccgctg atcatcggca tgacggtggt cagtattggt acatcgttac
    49501 cagaaatcat cgtctcgctt gccgcgtctc tgcacgaaca acgcgattta gccgttggta
    49561 cagccctcgg ctcaaacatt atcaatatat tgctgatcct cggtctggct gcgctggttc
    49621 gtccttttac cgtccattcc gatgttctgc gccgtgaatt acccttaatg ttgttggtca
    49681 gcgtggtggc cggttccgta ctctatgacg gacaacttag tcgaagcgat ggtatctttc
    49741 tcctctttct ggctgtgcta tggctgctgt tcattgttaa acttgcacgt caggggactg
    49801 acagcctgac cagagagcag cttgcagagc tgccgcgtga cggcggattg cccgtcgcgt
    49861 ttttatggct cggcattgcg cttatcatca tgccagtggc cacgcggatg gtggttgata
    49921 acgccacggt gctggcgaat tactttgcca tcagcgagtt gacgatggct ctgacggcaa
    49981 ttgctatcgg aaccagtctg ccggaactgg caaccgcaat agcgggggtt cgcaaaggtg
    50041 aaaacgacat tgctgtcgga aatatcattg gcgcaaacat ttttaatatt gtcatcgtgt
    50101 tgggtttacc cgcgctgata acgccaggag agattgatcc actggcgtac agtcgtgact
    50161 acagcgtgat gttgctggtg agcattattt ttgcgttgct gtgctggcgg cgctccccgc
    50221 aaccgggccg tggtgtaggg gtattattaa ctggcggatt tatcgtatgg ctggcgatgt
    50281 tgtactggtt atcgccaata ctcgttgaat aactggaaac gcattatgtc gcacgtagag
    50341 ttacaaccgg gttttgactt tcagcaagca ggtaaagaag tcctggcgat tgaacgtgaa
    50401 tgcctggcgg agcttgatca atacatcaat cagaatttca cgcttgcctg tgaaaagatg
    50461 ttctggtgta aagggaaagt tgtcgtcatg gggatgggga aatcggggca catcgggcgc
    50521 aaaatggccg caacgtttgc cagcaccggt acaccttcat ttttcgtcca tcctagtgaa
    50581 gccgcgcatg gtgatttagg catggtcacc ccacaggatg tggtgattgc tatctctaac
    50641 tcaggtgaat ccagcgaaat cacggcctta attccagtgc ttaagcgtct tcacgtaccg
    50701 ttaatctgca tcaccggtcg cccggagagc agcatggcgc gcgccgcaga tgtgcatctg
    50761 tgtgttaaag tagcgaaaga agcctgtccg ttagggctgg caccgaccag cagcaccacc
    50821 gccacgctgg ttatgggcga tgccctcgct gtcgcgctgt taaaagcacg cggctttact
    50881 gctgaagatt ttgcgctctc acacccaggc ggcgcactgg gtcgtaaact tctgctgcgc
    50941 gtaaacgata ttatgcatac gggcgatgag atcccgcatg ttaagaaaac ggccagtctg
    51001 cgtgacgcat tgctggaagt tacccgcaaa aatcttggta tgactgtcat ttgcgatgac
    51061 aatatgatga ttgaaggcat ctttaccgac ggtgatttac gccgtgtctt cgatatgggc
    51121 gtggatgttc gtcagttaag tattgccgat gtgatgacgc cggggggaat acgtgtgcgc
    51181 cctggcattc tggccgttga ggcactgaac ttaatgcagt cccgccatat cacctccgtg
    51241 atggttgccg atggcgacca tttactcggt gtgttacata tgcatgattt actgcgtgca
    51301 ggcgtagtgt aaagattcaa ggataaacaa caatgagcaa agcaggtgcg tcgcttgcga
    51361 cctgttacgg ccctgtcagc gccgacgtta tggcaaaagc agagaacatt cgtctgctga
    51421 tcctcgacgt tgatggcgta ctgtcagatg gcctgattta tatgggcaat aacggcgaag
    51481 agctgaaagc gttcaatgtt cgtgacggtt atggcattcg ttgtgcgctc acctctgata
    51541 ttgaagtcgc tatcattacc gggcgaaagg ctaaactggt agaagatcgt tgtgccacat
    51601 tggggatcac tcacttgtat caggggcagt caaacaaact gatcgccttt agcgatttgc
    51661 tggaaaaatt ggcgattgcc ccggaaaatg tggcttatgt tggcgatgat ctcatcgact
    51721 ggccggtaat ggaaaaagtg ggtttaagcg tcgccgtggc cgatgcgcat ccactgttga
    51781 tcccgcgcgc cgattacgtg acgcgcattg ctggcggtcg tggcgcagtg cgcgaagttt
    51841 gcgacttatt actcctggcg cagggcaaac tggatgaagc caaagggcaa tcgatatgag
    51901 taaagccaga cgttgggtta tcattgtgct atcactggcg gttctggtga tgatcggcat
    51961 taatatggcc gaaaaagacg ataccgccca ggtggtcgtc aacaacaatg atcccaccta
    52021 taaaagcgag catacggaca cgctcgtcta taacccagaa ggggcactaa gctatcgatt
    52081 gattgctcaa cacgttgaat attattccga tcaggccgtt tcgtggttta cgcagccggt
    52141 acttaccacg tttgataagg ataaaatccc gacatggtcc gtaaaagcag ataaagccaa
    52201 gctgaccaat gaccggatgc tctatttata tggacacgtt gaagtcaacg cacttgtgcc
    52261 agactctcaa cttcgcagaa tcacgacgga taacgcgcag atcaatctgg tgacgcagga
    52321 tgttacctct gaagacctcg tcacgttata cggaacaaca tttaactcca gcggtctgaa
    52381 aatgcgcggc aacttacgca gcaagaacgc cgagctgatt gaaaaggtta gaacatccta
    52441 tgaaattcaa aacaaacaaa ctcagcctta atcttgtgct tgccagctca cttctggccg
    52501 ccagcattcc ggcatttgcc gtaaccggag acactgatca gccgatccac attgaatcgg
    52561 accagcaatc tcttgatatg caaggcaacg tggttacctt taccggtaat gtcatcgtca
    52621 cccagggcac catcaaaatt aatgccgaca aagtggtcgt tacccgtccg ggcggcgaac
    52681 aaggtaaaga agtgattgac ggatacggta aaccggcaac gttctaccag atgcaggaca
    52741 acggtaaacc cgttgaaggt cacgcttccc agatgcacta cgaactggca aaagattttg
    52801 tcgttctgac gggtaatgct tatctgcagc aggtcgatag caacattaag ggcgataaga
    52861 tcacttacct ggtgaaagag cagaaaatgc aggctttcag cgacaaaggc aagcgcgtaa
    52921 ccaccgttct ggtgccgtcg cagctgcagg acaaaaacaa caaaggccag accccggcac
    52981 agaagaaggg taattaattc gttatggcaa cattaactgc aaagaacctt gcaaaagcct
    53041 ataaaggccg tcgcgtggta gaagacgtca gcctgaccgt caactccggg gaaattgtcg
    53101 gtctgctggg gccaaacggt gccggtaaga ccaccacttt ctacatggtt gtaggcattg
    53161 tgccgcgcga tgcgggcaac atcattattg atgatgacga tatcagtctg ctgcctctgc
    53221 atgcacgcgc gcgccgcggt atcggctatc tgccacagga agcctccatt ttccgtcgcc
    53281 tcagcgttta cgataacctg atggcggtac tgcaaattcg tgacgacttg tctgctgaac
    53341 aacgtgaaga ccgcgcgaac gagctgatgg aagagtttca cattgagcac ctgcgtgaca
    53401 gcatggggca gtcactctcc gggggtgaac gtcgccgtgt agaaattgcc cgcgcactgg
    53461 ctgcgaatcc gaaatttatt ctgctcgacg aaccgtttgc cggggttgac ccgatctcgg
    53521 ttatcgacat taaacgcatc attgaacacc tgcgcgacag cggcctgggc gtgctgatca
    53581 ctgaccacaa cgtgcgtgaa acactggcgg tttgtgaacg cgcttatatc gtcagtcagg
    53641 ggcatttgat cgcccacggc acgcctacag aaatcttaca agacgaacac gttaagcgtg
    53701 tataccttgg ggaagacttc agactctgat agggtagaag tttgcgacgt tttagcagga
    53761 gagtacgatt ctgaacatga agcaaggttt gcaactcagg cttagccaac aactggcgat
    53821 gacgccacag ctccaacagg caattcgtct gttgcagttg tcgacgctgg aacttcagca
    53881 ggagctacag caggcgctgg agagtaatcc gctgcttgag caaatcgaca ctcatgaaga
    53941 aatcgacacc cgcgaaacgc aagacagtga aacgctggac accgccgacg cgctcgaaca
    54001 aaaagagatg ccggaagagc tgccgctcga tgccagttgg gacaccattt acaccgctgg
    54061 tacaccatcc ggcaccagcg gtgactacat tgacgacgag ctgccggtct accagggcga
    54121 aacgacgcag accttgcagg attacctgat gtggcaggtc gagctgacac cgttttccga
    54181 cactgaccgc gctattgcta cctctatcgt cgatgccgtt gatgaaaccg gttatctgac
    54241 tgtcccgctg gaagatattc tcgaaagtat gggcgatgaa gagatcgaca tcgacgaggt
    54301 tgaagccgtc cttaagcgga tccaacggtt tgacccggtc ggtgtagcgg caaaagatct
    54361 gcgtgactgc ctgctaatcc aactctccca attcgataag accacgccgt ggctggaaga
    54421 ggccagattg atcattagcg atcatctcga tctgttagcc aatcacgact tccgcacttt
    54481 aatgcgcgtc acgcgtctga aagaagatgt gctgaaagaa gccgtcaatc tgatccagtc
    54541 gctcgatccg cgccccgggc agtcgatcca gactggcgaa cctgagtatg tcattccaga
    54601 tgtgctggtg cgtaagcata acggtcactg gacggtagaa ctcaacagtg acagcattcc
    54661 gcgtctgcaa atcaaccagc actacgcctc gatgtgcaat aacgcgcgca acgatggtga
    54721 cagccagttt atccgcagca atctgcagga tgccaaatgg ttgatcaaga gtctggaaag
    54781 ccgtaacgat acgctactgc gcgtgagtcg ctgtatcgtt gaacagcagc aagccttctt
    54841 tgagcaaggt gaagaatata tgaaaccgat ggtactggcc gatatcgccc aggctgtcga
    54901 aatgcatgaa tcgacgatat ctcgcgtgac cacgcaaaaa tacctgcata gtccacgagg
    54961 catttttgaa ctgaagtatt tcttttccag tcacgtcaat accgagggcg gcggcgaagc
    55021 ttcctccacg gcgattcgtg cactggtgaa gaaattaatc gcggcggaaa acccagcgaa
    55081 accgttgagc gacagcaagt taacctcttt gctgtcggaa caaggtatca tggtggcacg
    55141 ccgcactgtt gcgaagtacc gagagtcttt atccattccg ccgtcaaacc agcgtaaaca
    55201 gctcgtttga cccaaccgat aaggaagaca ctatgcagct caacattacc ggaaataacg
    55261 tcgagatcac cgaggcactg cgcgaatttg ttacagctaa atttgccaaa cttgagcaat
    55321 attttgaccg aatcaaccag gtctatgttg ttctgaaagt ggagaaagtc acccacacct
    55381 cagatgcaac actgcatgta aacggtggtg aaattcatgc cagcgcggaa ggtcaggaca
    55441 tgtacgctgc cattgatggc ttaattgata agctggcacg ccagttgacc aaacataaag
    55501 ataaactgaa acaacactaa ttgtccgggc aattagcatg tgcatggcgg cctgttgtgc
    55561 ggcacaacgg gccatttgta cggttaatgc tccgagcctg ttccactgtt tgagtgggca
    55621 ggttcttagg tgaaattatg acaaataatg atacaactct acagcttagc agtgttctta
    55681 acagggaatg tacgcgaagc cgcgtccact gtcagagcaa aaaacgcgcc ctggaaatca
    55741 tcagcgagct ggcggcgaaa caacttagcc tgccgcctca ggtggttttt gaagctatcc
    55801 tgacgcggga aaaaatgggc agtaccggta tcggcaatgg tattgccatt ccgcatggca
    55861 aacttgaaga agatactctg cgtgccgttg gcgttttcgt tcagctcgaa acgcctatag
    55921 ctttcgatgc catcgacaac caaccggtgg acttactttt tgccctgctg gtgccggcag
    55981 accaaactaa aacgcacctg catactctgt cgctggtggc gaaacgtctg gcggacaaaa
    56041 ccatctgccg ccgtttacgc gcagcccaga gcgatgaaga gctgtatcaa atcattacgg
    56101 ataccgaagg tactccggat gaagcgtagt tattcggtaa tgtctctttt agacgttgtg
    56161 aggagaaaca gtacatggta ctgatgatcg tcagcggacg ttcaggttca ggtaaatctg
    56221 tcgccctgcg tgcgctggaa gatatgggtt tttactgcgt ggataacctt cccgtagtgt
    56281 tgttacccga tctggctcga actctggccg atcgcgagat ttctgccgcc gtcagcattg
    56341 atgttcgtaa tatgccggag tcaccagaaa tattcgaaca ggcgatgagt aacctgcctg
    56401 acgctttctc accgcaacta ctgttcctgg atgccgaccg taatacctta attcgtcgtt
    56461 acagtgacac gcgccgactg catccgcttt ccagcaaaaa cctgtcgctg gaaagtgcta
    56521 tcgacaaaga aagcgatttg ctggagcctc tgcgttcgcg agcggatctg attgtcgata
    56581 cctcagaaat gtccgttcac gagctggctg aaatgctgcg tacccgtctg ctgggtaaac
    56641 gtgaacgcga actgaccatg gtctttgagt ctttcggctt caaacacggt atccctatcg
    56701 atgcagatta cgtctttgac gtgcgcttct tgccgaaccc gcactgggat ccgaaactgc
    56761 gtccaatgac aggtcttgat aaacctgtcg ccgcgttcct cgaccgccac acagaagtac
    56821 acaattttat ctaccagacg cgaagctatc ttgagctatg gttacctatg ctggaaacca
    56881 acaaccgtag ctacctgacg gtcgccattg gttgtaccgg cgggaagcac cgttcggtgt
    56941 atattgcaga gcaactggca gactacttcc gctcgcgcgg taaaaacgtc cagtcacgcc
    57001 atcgtacgct ggaaaaacgt aaaccatgac cgtcaagcaa actgttgaaa tcacaaacaa
    57061 gctgggcatg catgcccggc ctgcaatgaa gctgtttgaa ttaatgcagg gttttgacgc
    57121 tgaagtgctg ttacgtaatg acgaaggcac cgaggctgaa gccaacagcg ttattgcgct
    57181 gctgatgttg gattctgcca aaggacggca gattgaagtt gaagcgaccg gtccacagga
    57241 agaggaagca ctggccgccg ttatcgccct ctttaattct ggttttgatg aagattaatc
    57301 ttcatcactt tgacatacaa ctcccttcaa gctcccccga caataagaaa atcacgtact
    57361 gaaatcgttc tcaatcaacg tcatttgtac attttgtgcg cttttcactt tcagaagaac
    57421 cttaagaaaa ccttaagagg cattgtttag gttttgttta agttaatcga ccatactgga
    57481 gatcgtcaga aaatatttcc aggagatggc atgattcgct tatctgaaca aagtcccctg
    57541 ggcaccggaa gacatcggaa atgttatgcg catccggaag atgcccaacg ctgtatcaag
    57601 attgtctacc atcgtggcga tggtggcgat aaagagatcc gccgtgagtt aaagtactac
    57661 gcgcatcttg gtcgccggtt aaaagactgg agtggaatac cgcgctatca cggtaccgtt
    57721 gagacggatt gcggaaccgg ttatgtctac gatgtgattg ccgattttga cggcaagcct
    57781 tccattacgc tgaccgaatt tgctgaacaa tgccgttacg aagaagacat tgcacaactg
    57841 cgccagctac tgaaacagct gaagcgttat ttgcaggata accgtatcgt gacgatgtcg
    57901 ctgaagccgc agaatatcct ctgtcaccgc atcagtgagt cagaggttat cccggtcgtc
    57961 tgcgacaaca ttggtgaaag cacattgatt ccactggcga cctggtcgaa gtggtgttgt
    58021 ctgcgtaagc aagagcggtt atggaaacga tttatcgccc aaccggcgct ggctattgcc
    58081 ctgcagaaag atctgcaacc tcgtgagagc aaaacgctgg ccctcacttc gcgcgaagct
    58141 taatccagct ggtgttgttg cataaacggc tcaccgccta actgatacat ctgccgtaaa
    58201 atccacgcct gacggctacg cacgtagccc gatggcgagg agactttgaa acgaagtgga
    58261 ttaggtaata cagctgccag taatgcagct tccgaccggg taagtttgct cgcgggtttg
    58321 tggaaataac gttgtgccgc agcttcgacg ccaaacacac cgtcgccaaa ttcggcgata
    58381 ttcaggtaaa ccgtcaggat acgctttttg ctccagactg tttctatccc cagcgttaat
    58441 ccagcttcca gcccttttcg tacccagcta cgcccatccc ataaaaagag attttggccg
    58501 tctgctgaga aatcgttgaa gcaccgcgaa tacggttttc attgcgctcg ttgtgcgcca
    58561 gggctttctc aatggaagcg acatcaaagc cccagtgctc aggaaatttc tgatcttctg
    58621 cggcaatcac cgccagtccc atccacggcg agatttgatc catactgacc cagtcagaat
    58681 gtgccacgta acgaaaattg ccatgcagcc aggcgctgac ctgtcgctcg accattaccg
    58741 ctgagaaggg aacaggcgca acgctaaaca acgcgatgcc cccgccccag aaaacggcga
    58801 ggacaaccat taaccgcaaa agaaaacggc gaacgaaact aaacaccgtt aagcggcttt
    58861 tactcattca gccagaacca gcacgcggga aaccagctta tcaatgccgc tcgccgcttc
    58921 tgcaatgttc tgcgccagca tatatgctgg ggtggtgaca atcttattgt cttcatcaac
    58981 cacgatatca tcgacaggac acggcacatg ctccgcgccc atctcttcca gcacttctgc
    59041 ggtatcgata tcagtaccga tggtcaaacg cagcgggaaa tcgaaaattt tcggcagcat
    59101 cgccggggca atacacataa aaccaagcgg ttttccggcc tgatgcatcg cttgtgccag
    59161 cgcctttaat tcacggtcaa cggtgcattc gctaccaaga ctggcaaaat tgcttaaatt
    59221 cttcgccgcg ccaaaccccc ccggcacaat caacgcatcc agttcagcgg catcggcctg
    59281 ggccagagga cggatttcac cgcgcgttat tcgtgccgct tcaatcagca cattgcgcgt
    59341 ttccgtcatc gcttcgccag ttaaatggtt gataacatca acctgctgct tatccggtgc
    59401 aaagcagacc gcctgcgcac cgctgcgtga aatagctaac aacgtcaaca ccgcttcatg
    59461 aatttcagaa ccgtcataga cgccgcatcc gctcagaatt acgccaattt tcttcattgt
    59521 gatcatcctt tcgcaattga ctgaaacaca ttaatttttt taataaaaat ggtacgcatc
    59581 acacatttaa ctgattcatg taacaaatca tttaagtttt gctatcttaa ctgcgtgcgg
    59641 cctgaaaaac agtgctgtgc ccttgtaact catcataata atttacggcg cagccaagat
    59701 ttccctggtg ttggcgcagt attcgcgcac cccggtctag ccggggtcat tttttagtgg
    59761 cttttgccac ccacgctttc agcacttcta cgtcgtgacg ccactcttct ttcatctctt
    59821 caatccattc accgacgtta tcttcccagg cgggaaggtc aggagactga atttgctgac
    59881 ccagctgttg cagatggcgt aaccccactg accccgccgc acctttaatt ttatgtcctt
    59941 cctcaacaat gcctttttta tcctgcgccg tcagattcga ctccagcacg ctgacatagc
    60001 ccggcatcat cttctcaaac accgctaacc cgtcggtgat cagcttcggt cctacaagtt
    60061 cgagatactg ttccagcatg ggaatatcga gcaatgcttc tgatttactg ttctcttctg
    60121 tcgtcaccgt actctcctca tcatcctggg tatcccagaa tttcttgatc atcgcggtta
    60181 gcgccggaac cgaaagcggc ttactcagca catcatccat tccagcattg aggtactctt
    60241 gtttgtcttt cagcacgtta gcggttaagg ccaccagcgg cggtaaatcc tcgcgcggat
    60301 aacgcttcgt cagttcacga gagatatcca gcccggtcat atctggcaac tgaatatcca
    60361 gcaacaccag gtcgtattcg cccggtttaa acatctccag cgccgccttg ccggtcatgg
    60421 cgacatcaac gctgttacct aatttttcca gcacagaacg cgcgacaatc acgttcagtt
    60481 caatgtcttc caccagcagc acattcagcg ccggtaaagg catatcgtct tcatcaaacg
    60541 catcatcgac ctcttctgcc accgacggtg cgtggatcgt caacgtaaag gttgaacctt
    60601 tgccctgttc actggtaacc gtaatatcgc cgcccatatt tttcgccaga cgacgagaaa
    60661 cggccagacc aataccggtg ccggtggcag gtttaccgcc atgactgtct ttcacctggt
    60721 aatacatggc gaagatttta tccagctcat cctgcggaat gccgatacca gaatcttcca
    60781 cttcaaaatg cagcatatcg ccttcatcgt agcgcacgcg cacggtaacc tggccttgct
    60841 gggtgaattt gacggcgtta ctgatgaggt tccacaggat ctgccgtaaa cgcgtcccgt
    60901 cggtaatgac ctgatgcggt aatggcagcg taggctccag gttaaagcgc aatccttttt
    60961 gttgcgcctg caatgcggag agattttcca gatcggcaag gaagctggtg aaatcaaccg
    61021 gctgattatc aagctggacc ttgcgccgtt ccatcttatc catgtcgata atatcgttaa
    61081 agatattccc cagcgtgacg gccgaaacat ggatagtttt gagatatttt tcctgctcgg
    61141 cggtgagttc ggtatccagc agaatgcggc tcaggccgac gataccgttc agcggtgtac
    61201 gcaattcgtg actgatggtg gaaataaacg tcgttttgtc gcggctggcg cgttcaagcg
    61261 catcctgata ccgcttacgc tcggtaatgt cgcgaccaaa gcccatcaaa ccgtgacgtt
    61321 tacccacgcg gtcgtagtac ggcactttac ggatttcaaa gcaggctttg cgcccgtccg
    61381 ggtaatccag ccactgttca taggtcagtg acacattatg acggaacact ttttcatcgg
    61441 tttcaatgac ttttgcggcg gcttccggtg agtaaacatc agcaggtttc aggtgaacca
    61501 gttgtttttc gctttttccg gtcagcagct ccatcgcgcg gttacagccg gaaaactctt
    61561 tatcttcgtt acgataaaaa accaagtcgg gtgaagcatc aaggaaggaa cgtaagaatg
    61621 aggattgctg ctcgagctga atttgtgtct cttcgcgctc tttgatttca attttcagtt
    61681 ggccgaaggt ttcctgcagt tctgcttccg ctttttcacg aacggcaatt tcctgattta
    61741 gctgggcaat attatctttt aactgaacgt tgaggctcaa atcgcgctcg cgcatctcct
    61801 ccagtttttg caccagccgt gacagacgtt gtcgtgactc ctccagttgc tcgacgacca
    61861 ccgatagaaa gtagaccgcc cacggcgtaa tcagcaaacc aaagaagata gaacgaataa
    61921 catcaatgct ttcgacctga ccatgcagca ccatggttac cgccatttgt accacaatgg
    61981 caagaacgac gagggccagc gccagcaaca ttgagaagcg caccagacct aacttcatca
    62041 tcaggtcaac ataatactgc gccagcagac gaatttgctt cattagggaa ttccttcacg
    62101 acaacctgcc ccaataatac ccaattctga cagttacgtt ataaattgtg tgagaaatgc
    62161 ggaattcccc tcaacgacct actccgttgg aggtagccac ggacgtccca gcgccgatcc
    62221 ctgtacgcca tgttcattca gatagcgatc cagctcgacc attcccgtcc atcgattttc
    62281 gcaccacagc ggagcaagca gcgtcgggcg acgggcgctg gcggaaatgc ggtggtagat
    62341 cacttccggt ggcgtatggc gaatcatttc tccggcagtg agcgtataat cctccagttc
    62401 aataccgttc aaacgccccg cttcccaggc tttcgccata atgctgcctt tcacaatatg
    62461 cagcggatgc agctttatgc cgtccacgct ggtttcaacc actcgctcca gcgtttgcaa
    62521 acattcagcc tgcccttcgc caggcaggcc gacaattaag tgggaacaca ccttcagccc
    62581 gcgctgacgt gccagctggg ttgtacgctg ataacaggca aaatcatggc cgcggttgat
    62641 gcgatgcagt gttttgtcgt gggcggtttg tagccccagc tccagccaca cttcgtaacc
    62701 ctggtcctta tattcgcaaa gcagatccag caccgcatcc ggcacgcagt ccgggcgggt
    62761 accaacacac aaaccgacaa tattggcctg gctcaccgcc tgctgataca tagaacgcag
    62821 cacctgaact tccgcaaagg tgctggtata cgcctgaaag taggccagat agcgtttagc
    62881 gcggttaact aaattcgcct ggtgcgccag ttgctcggca atggaacgat gctgctgcgc
    62941 ttcatcggca aacgaggcaa cattacagaa tgtgcagcca ccacgcccga tggtaccgtc
    63001 acggttaggg cagctaaaac cgccgtgcag cgtcagctta tgcacctttt gcccataacg
    63061 acgggtgaga tcaccaccaa acatattgac taatttctgt aactgcataa tctgatagac
    63121 cgcgccttga aaagaggcca aagcctgcca tttttagctc aattcggcga tgacctggat
    63181 caatcgtccc ggcctgcttt tatcaactgc ataatcaatc aaaattaccg aaatttcatg
    63241 cacaatcaca taaatcactt ttgcttatct tgtgtcagat ttttttatct ccttatggat
    63301 tttaggcaaa aacagtagca tgaaacgtca ttaccaatta aggcagtata aaatgctggt
    63361 tttctcgtca gttcaaggca ggataagggt taacacgcct ttatgacagt caggaattga
    63421 ctgtttctct aacgactctc cttttagcct taaagataaa atctatttta atttcagcta
    63481 tttaataaag aattttgcgc taaagcacat ttctgtacca ataagcttgc catttgacct
    63541 gtatcagctt tcccgataag ttggaaatcc gctggaagct ttctggatga gcagcctgct
    63601 catcatattt atgcagtaat tgagatcccc tcttcaccgt attaaccgat gcgaaaagga
    63661 caacaagggg gcgaatgcga ggcgcgcgta tgacacgcaa accccgtcgc cacgctcttt
    63721 ctgtgcccgt gcgcagcggt tcggaagtgg ggttcccgca gagcctgggg gaggttcacg
    63781 atatgttgta cgataaatcc cttgagaggg ataactgtgg tttcggcctg atcgcccaca
    63841 tagaaggcga acctagccac aaggtagtgc gtactgcaat acacgcactg gcccgcatgc
    63901 agcaccgtgg cgcgattctc gccgatggta aaaccggcga cggttgcggc ttgctgttac
    63961 aaaaaccgga tcgctttttt cgcatcgttg cgcaggagcg cggctggcgt ttagcaaaaa
    64021 actacgctgt cgggatgctc ttcctgaata aagatcctga actcgccgct gccgcacgcc
    64081 gcatcgttga agaagaactg caacgcgaaa ccttgtcgat tgtgggctgg cgtgatgtcc
    64141 ccactaacga aggcgtgctg ggtgaaatcg ccctctcctc tctgccacgc attgagcaaa
    64201 tttttgtgaa cgccccggca ggctggcgtc cacgcgatat ggagcgccgt ctgtttatcg
    64261 cccgccgccg cattgaaaag cgtctcgaag ccgacaaaga cttctacgtc tgtagcctgt
    64321 cgaatctggt gaacatctat aaaggtctgt gtatgccggc ggatctgccg cgcttttatc
    64381 tggatcttgc ggacctgcgt ctggaatcgg ccatttgcct gttccaccag cgcttctcca
    64441 ctaacaccgt accgcgctgg ccgctggcgc aaccgttccg ctatctggcg cataacggtg
    64501 aaatcaacac catcaccggt aaccgccaat gggcgcgtgc gcgtacctat aaattccaga
    64561 cgccgctgat ccctgacctg cacgacgccg cgccattcgt caacgaaacc ggttctgact
    64621 ccagttccat ggataacatg ctggaactgc tgctggcagg cgggatggat atcatccgcg
    64681 ccatgcgcct gttagtgcca cccgcctggc agaacaaccc ggatatggac ccggaactgc
    64741 gtgccttctt tgactttaac tccatgcata tggagccgtg ggatggtccg gcgggcatcg
    64801 tgatgtccga tggtcgcttc gctgcctgta acctcgaccg taacggtctg cgtccggcac
    64861 gctacgtcat taccaaagac aagctcatca cctgcgcctc tgaagtcggt atctgggatt
    64921 accagcctga cgaagtggtc gaaaaaggcc gcgtcgggcc aggcgaactg atggtgatcg
    64981 acactcgcag tgggcgtatt ctgcactcgg cagaaaccga tgacgatctg aaaagccgcc
    65041 acccttataa agagtggatg gagaaaaacg tccgccgact ggtaccgttt gaagatctgc
    65101 ccgatgaaga agtgggtagc cgcgaactgg acgacgacac gcttgccagc taccagaaac
    65161 agtttaacta cagcgcggaa gagctggact ccgtaattcg cgtactgggc gaaaacggtc
    65221 aggaagcggt cggttcgatg ggcgatgata ccccattcgc cgtgctctcc agtcagccgc
    65281 gcattattta cgactacttc cgccagcagt ttgcccaggt gactaacccg ccaatcgacc
    65341 cgctgcgtga agcgcatgtt atgtcgctcg ccaccagtat cggtcgtgaa atgaacgtct
    65401 tttgcgaagc agagggccag gcgcaccgtt taagctttaa atcgccgatt ctgctctact
    65461 ccgatttcaa acagctcacg acgatgaaag aggagcacta ccgcgcagat acgctggata
    65521 tcacctttga cgtcactaaa accacgctcg aagcgacagt caaagagcta tgcgacaaag
    65581 ccgaaaaaat ggtacgtagc ggcaccgtgt tgctggtgct ctccgaccgg aatatcgcta
    65641 aagatcgcct gccggttccg gctccgatgg cggttggcgc gatccagacc cgtctggtcg
    65701 atcaaagcct gcgttgcgat gccaacatca tcgtcgaaac cgccagcgcc cgcgatccgc
    65761 accacttcgc cgtgttgctg ggtttcggcg cgacggctat ttatccgtac cttgcctatg
    65821 aaacgctggg ccgcctggta gacacccacg cgattgccaa agattatcgt accgtgatgc
    65881 tcaactaccg taacggcatc aacaaaggct tgtacaaaat catgtccaaa atgggcatct
    65941 ccaccatcgc ctcttaccgc tgctcgaaac tgtttgaagc ggtcggtcta cacgatgatg
    66001 tagtgggcct gtgcttccag ggcgcggtca gccgcattgg tggggcaagc tttgaagact
    66061 tccagcagga tctgctgaat ctgtcgaaac gtgcctggct ggcgcgcaag ccgattagcc
    66121 agggcggcct gctgaaatac gtccacggcg gcgaatacca cgcctacaac ccggacgtgg
    66181 tgcgcacgct gcaacaggcg gtacaaagcg gcgagtacag cgactatcag gaatacgcga
    66241 agctggttaa tgagcgtccg gcaaccacgc tgcgcgatct gctggcaatt acgccgggtg
    66301 aaaacgcggt caacattgct gatgttgaac cggcaagcga actgtttaaa cgctttgata
    66361 ccgccgcgat gtctatcggc gcgttaagcc cggaagccca cgaggcgctg gcggaagcga
    66421 tgaatagcat cggcggtaac tcgaactccg gtgaaggcgg cgaagacccg gcgcgctatg
    66481 gcactaacaa agtgtcgcgc atcaagcagg tggcttctgg tcgcttcggc gttactccgg
    66541 cgtatctggt caatgccgac gtcattcaga ttaaagtcgc ccagggcgcg aagccgggcg
    66601 aaggtggtca gttgccgggc gacaaagtca ctccttacat cgccaaactg cgctattcgg
    66661 tgcccggagt gacgctgatc tccccgccgc cgcaccacga tatctactct atcgaagact
    66721 tagcgcagct tattttcgac ctcaagcagg ttaacccgaa agcgatgatc tccgttaagc
    66781 tggtgtccga accaggcgtt ggcaccatcg cgactggcgt ggcgaaagct tatgcggact
    66841 tgatcaccat cgcgggctat gacggcggca ccggcgcaag cccgctgtcg tcagtgaaat
    66901 atgcaggctg cccgtgggaa ctcggtctgg tggaaaccca acaggcgctg gttgccaacg
    66961 gcttgcgtca taaaattcgt ttgcaggttg atggcggcct gaaaacgggt gtcgatatca
    67021 tcaaagcggc gattctcggc gcagaaagct tcggcttcgg cactggccca atggtggcgc
    67081 tcggctgtaa atatctgcgt atttgccacc tgaacaactg cgccacgggt gtagcaactc
    67141 aggatgacaa actgcgtaag aatcactatc acggcctgcc gttcaaggtg actaattact
    67201 ttgagtttat cgcccgtgaa acccgcgagc tgatggcaca gctgggagta acgcgtctgg
    67261 tggatctgat tggtcgcacc gacctgctga aagagctgga cggtttcacc gccaaacagc
    67321 agaaactggc gctgtcgaag ctgctggaga ctgccgagcc acatgcaggt aagtcactct
    67381 actgcactga aaacaacccg ccgtttgata acggcctact gaacgcgcag ttgctgcaac
    67441 aggcgaaacc gtttgtcgat gaacgccaga gcaaaacctt ctggttcgat atccgcaaca
    67501 ccgaccgttc tgtcggcgcg tcgctttcag gctatatcgc ccagacgcac ggcgatcagg
    67561 ggctggcagc cgatcctatc aaagcgtact tcaacggcac cgcaggccag agtttcggtg
    67621 tgtggaacgc gggcggcgtg gaactgtacc tgaccgggga tgccaacgac tatgtcggta
    67681 aaggcatggc gggcggctta atcgccattc gtcctccggt tggttccgcc ttccgcagcc
    67741 atgaagcaag cattatcggc aacacctgcc tgtatggcgc gaccggtggt cgtctgtatg
    67801 ccgcaggccg cgcgggtgaa cgtttcggcg tgcggaactc cggtgctatc accgtggtag
    67861 aaggcattgg cgacaacggc tgtgaatata tgacgggcgg tattgtctgt attctgggta
    67921 aaaccggcgt taacttcggc gcgggcatga ccggcggctt cgcttacgtt ctcgatgaaa
    67981 gcggtgattt ccgcaaacgc gttaacccgg aactggtcga agtcttaagc gtcgacgatc
    68041 tggcgatcca tgaagagcat ctgcgcggtc ttatcaccga gcatgttcag cataccggtt
    68101 ctcagcgcgg tgaagagatt ctggcgaact ggtcaacctt cgccacgaaa ttcgcgctgg
    68161 ttaaaccgaa gtccagtgat gtaaaagcac tgctgggtca ccgtagtcgt agcgcagcag
    68221 agttgcgcgt gcaggcgcag taaggggtag caacaatgag tcagaatgtt tatcaattta
    68281 tcgacctgca gcgcgttgat ccgccaaaga aaccgctgaa gatccgtaaa attgagtttg
    68341 ttgaaattta cgagccgttt tccgaaggcc aggccaaagc gcaggctgac cgctgtctgt
    68401 cgtgcggcaa cccatactgc gagtggaaat gcccggtaca caactacatc ccaaactggc
    68461 tgaagctcgc caacgagggg cgtatttttg aagcggcaga actgtcgcat cagaccaaca
    68521 cactgccgga agtatgcgga cgtgtctgcc cgcaagaccg tctgtgcgaa ggttcctgca
    68581 ccctgaacga tgagttcggt gcggtgacca tcggcaacat tgagcgttat atcaatgata
    68641 aagcgttcga aatgggctgg cgtccggata tgtccggcgt gaagcaaacc ggtaaaaaag
    68701 tggcgattat cggtgcaggc ccggcaggtc tggcgtgtgc ggatgtcctg acgcgtaacg
    68761 gcgtaaaagc cgttgtcttc gaccgtcatc cagaaattgg cgggctgctg accttcggta
    68821 ttccggcctt caagctggaa aaagaggtaa tgacgcgtcg ccgtgaaatc ttcaccggca
    68881 tgggtattga attcaaactc aataccgaag tgggccgcga cgtacagctg gacgatctgc
    68941 tgagtgatta cgatgccgtg ttccttggcg ttgggactta tcagtcaatg cgcggcgggc
    69001 tggaaaacga agacgccgat ggcgtgtacg cagcgctgcc gttccttatc gccaacacca
    69061 aacagttaat gggctttggt gaaacccgcg acgaaccgtt cgtcagcatg gaaggcaaac
    69121 gcgtggtagt acttggcggt ggcgacactg cgatggactg cgtgcgcacg tctgtacgcc
    69181 agggggcaaa gcacgttacc tgtgcctatc gtcgtgatga agagaacatg ccgggttccc
    69241 gccgcgaagt gaaaaacgcg cgggaagaag gcgtagagtt caaattcaac gtccagccac
    69301 tggggattga agtgaacggt aacggcaaag tcagcggcgt aaaaatggta cgt
//
LOCUS       DAJTQQ010000007        67673 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000007 DAJTQQ010000000
VERSION     DAJTQQ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.007"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..229
                     /locus_tag="OO309_000528"
     CDS             <1..229
                     /locus_tag="OO309_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCR5845793.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            624..1263
                     /locus_tag="OO309_000529"
                     /pseudo
     CDS             624..1263
                     /locus_tag="OO309_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1366..1689
                     /locus_tag="OO309_000530"
     CDS             1366..1689
                     /locus_tag="OO309_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HCR5845794.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="OO309_000531"
     CDS             complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="OO309_000531"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR5845795.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="OO309_000532"
     CDS             complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="OO309_000532"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR5845796.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="OO309_000533"
     CDS             complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="OO309_000533"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR5845797.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="OO309_000534"
     CDS             complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="OO309_000534"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR5845798.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="OO309_000535"
     CDS             complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="OO309_000535"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR5845799.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="OO309_000536"
     CDS             complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="OO309_000536"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR5845800.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="OO309_000537"
     CDS             complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="OO309_000537"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR5845801.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="OO309_000538"
     CDS             complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="OO309_000538"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR5845802.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="OO309_000539"
     CDS             complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="OO309_000539"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR5845803.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="OO309_000540"
     CDS             complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="OO309_000540"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR5845804.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="OO309_000541"
     CDS             complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="OO309_000541"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR5845805.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15659..16321)
                     /gene="nuoB"
                     /locus_tag="OO309_000542"
     CDS             complement(15659..16321)
                     /gene="nuoB"
                     /locus_tag="OO309_000542"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR5845806.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="OO309_000543"
     CDS             complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="OO309_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR5845807.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="OO309_000544"
     CDS             complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="OO309_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR5845808.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19268..20485
                     /gene="alaA"
                     /locus_tag="OO309_000545"
     CDS             19268..20485
                     /gene="alaA"
                     /locus_tag="OO309_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR5845809.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20569..21168
                     /gene="yfbR"
                     /locus_tag="OO309_000546"
     CDS             20569..21168
                     /gene="yfbR"
                     /locus_tag="OO309_000546"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR5845810.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21227..23059)
                     /locus_tag="OO309_000547"
     CDS             complement(21227..23059)
                     /locus_tag="OO309_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR5845811.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23146..23796)
                     /gene="hxpA"
                     /locus_tag="OO309_000548"
     CDS             complement(23146..23796)
                     /gene="hxpA"
                     /locus_tag="OO309_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR5845812.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23807..24301)
                     /locus_tag="OO309_000549"
     CDS             complement(23807..24301)
                     /locus_tag="OO309_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR5845813.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24384..24839)
                     /locus_tag="OO309_000550"
     CDS             complement(24384..24839)
                     /locus_tag="OO309_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR5845814.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25177..26379
                     /gene="ackA"
                     /locus_tag="OO309_000551"
     CDS             25177..26379
                     /gene="ackA"
                     /locus_tag="OO309_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR5845815.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26454..28598
                     /gene="pta"
                     /locus_tag="OO309_000552"
     CDS             26454..28598
                     /gene="pta"
                     /locus_tag="OO309_000552"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR5845816.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28788..30308
                     /gene="yfcC"
                     /locus_tag="OO309_000553"
     CDS             28788..30308
                     /gene="yfcC"
                     /locus_tag="OO309_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR5845817.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30341..30883)
                     /gene="yfcD"
                     /locus_tag="OO309_000554"
     CDS             complement(30341..30883)
                     /gene="yfcD"
                     /locus_tag="OO309_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR5845818.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30941..31492)
                     /gene="yfcE"
                     /locus_tag="OO309_000555"
     CDS             complement(30941..31492)
                     /gene="yfcE"
                     /locus_tag="OO309_000555"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR5845819.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31548..32192)
                     /gene="yfcF"
                     /locus_tag="OO309_000556"
     CDS             complement(31548..32192)
                     /gene="yfcF"
                     /locus_tag="OO309_000556"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR5845820.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32328..32975
                     /gene="yfcG"
                     /locus_tag="OO309_000557"
     CDS             32328..32975
                     /gene="yfcG"
                     /locus_tag="OO309_000557"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR5845821.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFEAAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33032..33394
                     /gene="folX"
                     /locus_tag="OO309_000558"
     CDS             33032..33394
                     /gene="folX"
                     /locus_tag="OO309_000558"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR5845822.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33415..34308
                     /locus_tag="OO309_000559"
     CDS             33415..34308
                     /locus_tag="OO309_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR5845823.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34356..35246)
                     /gene="rpnB"
                     /locus_tag="OO309_000560"
     CDS             complement(34356..35246)
                     /gene="rpnB"
                     /locus_tag="OO309_000560"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR5845824.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35443..36216)
                     /gene="hisP"
                     /locus_tag="OO309_000561"
     CDS             complement(35443..36216)
                     /gene="hisP"
                     /locus_tag="OO309_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR5845825.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36224..36940)
                     /locus_tag="OO309_000562"
     CDS             complement(36224..36940)
                     /locus_tag="OO309_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5845826.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36937..37623)
                     /gene="hisQ"
                     /locus_tag="OO309_000563"
     CDS             complement(36937..37623)
                     /gene="hisQ"
                     /locus_tag="OO309_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR5845827.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37713..38495)
                     /gene="hisJ"
                     /locus_tag="OO309_000564"
     CDS             complement(37713..38495)
                     /gene="hisJ"
                     /locus_tag="OO309_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR5845828.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38716..39498)
                     /gene="argT"
                     /locus_tag="OO309_000565"
     CDS             complement(38716..39498)
                     /gene="argT"
                     /locus_tag="OO309_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR5845829.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39764..40333)
                     /gene="ubiX"
                     /locus_tag="OO309_000566"
     CDS             complement(39764..40333)
                     /gene="ubiX"
                     /locus_tag="OO309_000566"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR5845830.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40428..41945)
                     /gene="purF"
                     /locus_tag="OO309_000567"
     CDS             complement(40428..41945)
                     /gene="purF"
                     /locus_tag="OO309_000567"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR5845831.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41982..42470)
                     /gene="cvpA"
                     /locus_tag="OO309_000568"
     CDS             complement(41982..42470)
                     /gene="cvpA"
                     /locus_tag="OO309_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR5845832.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42729..43391)
                     /gene="dedD"
                     /locus_tag="OO309_000569"
     CDS             complement(42729..43391)
                     /gene="dedD"
                     /locus_tag="OO309_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR5845833.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43381..44649)
                     /gene="folC"
                     /locus_tag="OO309_000570"
     CDS             complement(43381..44649)
                     /gene="folC"
                     /locus_tag="OO309_000570"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR5845834.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44719..45633)
                     /gene="accD"
                     /locus_tag="OO309_000571"
     CDS             complement(44719..45633)
                     /gene="accD"
                     /locus_tag="OO309_000571"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR5845835.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45789..46448)
                     /locus_tag="OO309_000572"
     CDS             complement(45789..46448)
                     /locus_tag="OO309_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5845836.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46531..47343)
                     /gene="truA"
                     /locus_tag="OO309_000573"
     CDS             complement(46531..47343)
                     /gene="truA"
                     /locus_tag="OO309_000573"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR5845837.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47343..48356)
                     /locus_tag="OO309_000574"
     CDS             complement(47343..48356)
                     /locus_tag="OO309_000574"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5845838.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48422..49558)
                     /gene="pdxB"
                     /locus_tag="OO309_000575"
     CDS             complement(48422..49558)
                     /gene="pdxB"
                     /locus_tag="OO309_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR5845839.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49657..50652
                     /gene="flk"
                     /locus_tag="OO309_000576"
     CDS             49657..50652
                     /gene="flk"
                     /locus_tag="OO309_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR5845840.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50649..51827)
                     /locus_tag="OO309_000577"
     CDS             complement(50649..51827)
                     /locus_tag="OO309_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5845841.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52082..53323)
                     /gene="fabB"
                     /locus_tag="OO309_000578"
     CDS             complement(52082..53323)
                     /gene="fabB"
                     /locus_tag="OO309_000578"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR5845842.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53482..55488
                     /gene="mnmC"
                     /locus_tag="OO309_000579"
     CDS             53482..55488
                     /gene="mnmC"
                     /locus_tag="OO309_000579"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR5845843.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55609..55887)
                     /locus_tag="OO309_000580"
     CDS             complement(55609..55887)
                     /locus_tag="OO309_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR5845844.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55921..56469)
                     /gene="epmC"
                     /locus_tag="OO309_000581"
     CDS             complement(55921..56469)
                     /gene="epmC"
                     /locus_tag="OO309_000581"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR5845845.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56469..57278)
                     /locus_tag="OO309_000582"
     CDS             complement(56469..57278)
                     /locus_tag="OO309_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR5845846.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57278..58102)
                     /gene="mepA"
                     /locus_tag="OO309_000583"
     CDS             complement(57278..58102)
                     /gene="mepA"
                     /locus_tag="OO309_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR5845847.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58106..59191)
                     /gene="aroC"
                     /locus_tag="OO309_000584"
     CDS             complement(58106..59191)
                     /gene="aroC"
                     /locus_tag="OO309_000584"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR5845848.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59226..60158)
                     /gene="prmB"
                     /locus_tag="OO309_000585"
     CDS             complement(59226..60158)
                     /gene="prmB"
                     /locus_tag="OO309_000585"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5845849.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60324..60875
                     /gene="smrB"
                     /locus_tag="OO309_000586"
     CDS             60324..60875
                     /gene="smrB"
                     /locus_tag="OO309_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR5845850.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60979..61869)
                     /locus_tag="OO309_000587"
     CDS             complement(60979..61869)
                     /locus_tag="OO309_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR5845851.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61856..62380)
                     /locus_tag="OO309_000588"
     CDS             complement(61856..62380)
                     /locus_tag="OO309_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5845852.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62377..62847)
                     /locus_tag="OO309_000589"
     CDS             complement(62377..62847)
                     /locus_tag="OO309_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5845853.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62844..63392)
                     /locus_tag="OO309_000590"
     CDS             complement(62844..63392)
                     /locus_tag="OO309_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5845854.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63397..64119)
                     /locus_tag="OO309_000591"
                     /pseudo
     CDS             complement(63397..64119)
                     /locus_tag="OO309_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64139..64861)
                     /locus_tag="OO309_000592"
     CDS             complement(64139..64861)
                     /locus_tag="OO309_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5845855.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64921..66075)
                     /gene="yfcU"
                     /locus_tag="OO309_000593"
                     /pseudo
     CDS             complement(64921..66075)
                     /gene="yfcU"
                     /locus_tag="OO309_000593"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(66024..66779)
                     /locus_tag="OO309_000594"
     CDS             complement(66024..66779)
                     /locus_tag="OO309_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5845856.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDMKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDRD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKNGSGVATMPGGRYHH"
     gene            complement(66861..67424)
                     /locus_tag="OO309_000595"
     CDS             complement(66861..67424)
                     /locus_tag="OO309_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5845857.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16925 a        18281 c        16785 g        15682 t
ORIGIN      
        1 ggaaagcaat tctgacagcg ccccctatac gttattaaaa aactatgctg acagatttaa
       61 aaaatattca gcagaagagc agtacgaaat tgattttcaa catcgactgg taaacagaaa
      121 ttgttattta gataaatatg gcgatgcacg aatcaatgct tcttatacac aattagaaat
      181 aaaacactca caacccaaaa atagagcatc cggcaaaaga gtgagttaac atcattgtta
      241 atcaattcga ccagtcagta aatatgctga ctggtcgaac ataatttgct aaaaagtcat
      301 cactgactga tgtcagtccc accatctgcc agttcaatct acgggtattt ccagcatatc
      361 gcggcacgcg attgctggtc tgttgatgta gtggattgta gttgctgact tttcttaccc
      421 tcagatttct gccccacttg tcaattcaat ctgccaataa ataacataat tatattttta
      481 ttcctcacag aaattccttt ttaaaaatag cgaacccact gtaaaaaaca ctttttgtat
      541 acctctacca agcgtaataa aataaacgct cataattcat tataaataat aatgaaatga
      601 cgccttttat tgataattta catatgaaaa aattttcctt ttgttatatt atttctgttt
      661 tcactccctc tgattatttt taccaaccct gggtcactgc attatcgcca acgccccgac
      721 acgtaagtcc tgagcaatta gaaaaaacag ttcgttatct tacacaaact gtgcatccac
      781 gtaatgccgg caatattgat aatctgaata ggtccgcaga gtatataaaa gagttctttg
      841 tcagtagcgg tgccagagtt accgcgcagg gcgtccccga ttggctatta cgactctgcg
      901 cctggaagcc agaattaccc ttacccggca atgtcctggc tttatcccga tcggggagac
      961 tttattgctg tggtcggcag aatgcaggat atcaacgccg ttcgtcaggt aaaagcggca
     1021 ttgttgtcat cccgggattt atctgtttat tctatgaatg ccccagggtt tattcccggt
     1081 attgattttt ctgatcacct caattattgg caacacgata ttcccgccgt aatgattact
     1141 gacaccgctt tttatcgtaa taaacaatac cacttgcccg gtgataccgc agacagattg
     1201 aattatcaga aaatggctca ggtggtagat ggtgttataa ccttgttata caacagtaaa
     1261 taataaaaat ttctcgaggc gggtccagaa acagcgacat gtacatttta ttttccctcg
     1321 tctgttttaa gatgcgaaac ataaaattca atggaggaaa aataaatggg aatgattggc
     1381 tattttgcag aaattgactc agaaaagatt aaccaactgc ttgagtctac tgaaaaacct
     1441 ttgatggata atatacatga cacgctttca ggattacgcc gactggaatc actggatcga
     1501 agtgagctgc gtaaacagtt ttccattaag cgactcaatg aaatggagat ttatccggga
     1561 gtgacctttt cagaagagtt agaggggcaa ctctttgcct cgatcatgct tgatatggaa
     1621 aagctgatta gcgcgtatcg acgtatgctg cgacaaggga atcatgcgct gacggttatt
     1681 gttgggtgat ggtgtcagag cgccaaaaaa aaccgccgaa catgtcggcg gctttctgac
     1741 ttacaaacta acagtttaca tcagcggcat tgctaaccgc acaatactaa tcagcggttg
     1801 tggccataca cccagcacca gtaccaacag tgcagaaatc agcaccacga taccgcccgc
     1861 gctgtactgc cagtttgacg gtgcatcgcg acccggttgt tccggtgcgt gcaggtacag
     1921 gctcaccgcc acgcgcaggt agtagtagag gccgattgcc gaaccgacaa ccacggcacc
     1981 caccagccac cacaagtgtg cctggacacc gaccgccagc acgtagaact taccgataaa
     2041 gcccagcgtc atcgggatac cggccagaga cagcatcatc accgtcatca ctgccgcgag
     2101 gatcggacga tgccagaaca gaccgcggta agagaacagg gaatcagcat ccgggccacg
     2161 atacgggctg gacatcaggc tgaccacgcc gaacgcgccg aggctgctga acagataacc
     2221 ggccaggtaa acccctaccg cttccatcga catctcgccg gtttgcagcg caatcagcgc
     2281 caccagcaga tagccgaggt gagagataga tgagtaaccg agcagacgtt tgatgttggt
     2341 ctggctcaac gccatcaggt taccgaagat gatggaggca aaggcgataa tcgccagtac
     2401 tacgcgaatc gcttcgctgt cacccaccgg tgcgtagagg aacagacgca tcaccacacc
     2461 gaagatagcg attttgctcg ccgtcgccag gaaagtggaa accggcgcag gcgcgccctg
     2521 gtatacgtct ggcgtccaca ggtggaacgg caccagagag agtttgaagc cgaggccaac
     2581 aatcatcatg ccgaaacctg ccagcaacag cggctcgttg agcataccgt cgccgaggtt
     2641 tttacccagc gcgacaaacg acaggtcgcc agactgcgca tacaccagcg ccataccgaa
     2701 cagcaggaaa gaagacgctg cggcagaaag gatggtgtat ttgatactgg cttccagcga
     2761 acgtttctgg cggaaagcgt aaccgaccag gccaaacagc ggcaaagaga tcagttcaat
     2821 accgaggaac agagacgcca gatggtttgc attcgccagc aggatcccgc ccagcgcggc
     2881 aattaacacc aacaggtaga actcatcctt gttgtcgtta tagccttcaa gccacgggta
     2941 ggcgaaagta caggtggcga ggctcgccaa caataccagc ccggtgtaaa gcatggcgaa
     3001 accatcaacg cgcatcagcg gcgtaacgtc catagcgccc gcctggccaa caaaccagag
     3061 cgaaaccagc gccgcgttaa gcccaataac cgagagcgta gcgttgagga aatgattgcg
     3121 tcgccacgca atggagagca tcacaaccac caccgtcaag ccgacgatca gcaacggtag
     3181 cagtgcgatc aggttttgtg gagttattgt catggcgatt tacggccttg tagtagtaac
     3241 ggaattaaca aaccactgct ggatattgcc aatcgcggag tgcgaggtat ccagaatcgg
     3301 ctgcggatag aagcccagca gtaccagcag caccaccagc aacaggatca taaacagctc
     3361 acgcagcgac atccctggca gttcctggct ggcaatctgg cttttcgctt taccgaagta
     3421 agcgcgatgc aacatcgcca gcgaataaac agatgcaaag accagcccaa aggtagagat
     3481 aacggtaatc accgggacaa cctggaagct gccgaacaga atcataaatt cgccgacgaa
     3541 gttaccggtg ccaggcatcc caagcgttgc caccgcaaag aacagcgaca gtgctggcag
     3601 ccatttcatc ttgctccaca gaccgcccat catgcgcatg tcgcgggtat ggatacgttc
     3661 ataaagctga ccgcagagaa taaacagacc cgccgccgac aagccgtgcg caatcatctg
     3721 gattaccgcg ccctggtagg ccaactggct gccggtgtag attgcaatca gcacgaagcc
     3781 catgtgggaa accgaggtgt aggcgatcag acgtttgata tcggtctgag cgaaggtcat
     3841 ccacgcaccg tagaagatgc cgataacacc cagccacata gcgattggcg cgaactctgc
     3901 cgacgcgttc gggaacagcg gcagggagaa acgcaacaaa ccgtaagcgg cggttttcag
     3961 caagatcccc gcgaggtcca cggaaccggc ggtcggagcc tgggagtgcg catccggcag
     4021 ccagccatgc agcggaacca ccggcatttt gactgcgaag gcgatgaaga agcccagcat
     4081 caacagatat tccacaccac tggacattgg cgtattcagc agctcttcat agttgaaggt
     4141 ccagacgccg gtcgcattgt agtgaacaaa aaccagcgcc aggatggcga tcaacatcac
     4201 cagaccactc gcctgggtgt aaatgaagaa cttggttgcc gccgtgatac gcgttttacc
     4261 gtcagaggct ttatgccccc acagtgcgat caggaagtac atcggcacca gcatcatttc
     4321 ccagaagaag aagaacagga acatgtcgat ggcaaggaac acgccgataa cgccgcccag
     4381 gatccacatc aggttgaggt ggaagaagcc ctgatatttt tcgatctctt tccacgaaca
     4441 tagtaccgcc agcacaccga gcagaccggt cagcacgacc atcagcagcg acagaccgtc
     4501 aatggcgaga tgaatagaga taccaaaacg cgggatccac ggcatgtcga attcagactg
     4561 ccactgcgga attccggcgg attgcgtcag tgaataaccg ccctgcaacc acagttgcag
     4621 cgacagcgcc agcgtcaatc ccatggtgat cagcgcgatc cagcgtggca ccttgacgcc
     4681 aaagcgttcg gtctgccagc acaggaagcc gccaataaag ggaattaata ttagccaggg
     4741 tagtaacatg gcgatcttta ttccttgtaa aagtcccgtc aggaccggat tttcaacgaa
     4801 ttctcacgac agaaaattct cttcggactg ggggtagtgt cggatgcgcc gcgaaggcag
     4861 cttatccgac ctacgttctt atatgcccag gggcaatccc acaatcctta actcaacgca
     4921 gtaccatcaa cagtgccagc accacgaccg caccgatgct catggatgcc acataccagc
     4981 gcagataacc gttctcgctt aacagcagac ctttacctgc aaagcgggaa agaaccgccg
     5041 ggatgttcat cattgagttc agcggatcgc gtttcagcaa ccaggcaata cccaggaacg
     5101 gcttgacgaa cactttgtcg tacagccagt caaagcccca agcgttgtac caccaggtac
     5161 tcagcaggcg gcccggcgca ctgttggcga tggaggtcac cagagaacgt ttacccagcc
     5221 acagccaggc tgccagcaga atgccgacca ccgcgaccac gccagaggta atttccaggg
     5281 tcaacatgct gccgtgcgcc agttccgtcg tttgcggaag cacgccctgc agcggcggta
     5341 caatcagtgc gccaacgaag gtggaaagga tcagcagcac aatcagcggc aggctgtgag
     5401 ttaccccttt cacggcgtga gcgtgaattt gttcttttcc gtggaagacg atgaaaatca
     5461 tacggaaggt gtagagcgag gtcataaacg caccgaccag acctgccacc atcagattga
     5521 tatgaccatt cgccatcgca cccgcgagga tctcatcctt actgaagaag cccgcagtga
     5581 ccagcggcag tgccgacagt gctgcgccgc ccaccaggaa gcagagataa accagcggaa
     5641 tagatttacg cagaccgccc atcttgaaga tgttctgttc gtgatggcag gccagaatga
     5701 cggaaccgga tgccaggaac agcagcgctt taaagaacgc gtgagtcatc aagtggaaaa
     5761 tcgccgcatc ccatgcctgc acgccaagcg cgaggaacat gtagccaatc tggctcatgg
     5821 tagagtaagc gagaacacgt ttgatgtcgg tctgtaccag cgcggcaaaa ccggccagca
     5881 gcagcgtaac cgccccgaca atacccacca gatgcagaac ttccggcgtc atcaggaaca
     5941 ggccgtgggt acgggcgatc aggtagacac ccgcggttac catcgttgcg gcgtggatca
     6001 gcgcggagac aggcgtcggg cccgccatcg cgtcagcaag ccatgtctgc aacggcaact
     6061 gcgcagattt accgaccgca ccgcccagca gcatcagcgt cgcccacatc agcatgttat
     6121 tgccgtcagc aaagtgcgct ggtgccagtt ccaccatatc gcggaagttc agggtgccca
     6181 gttcgttgta aagaatgaac agtgcgaaag cgaggaaaac gtcacccaca cgggtcacga
     6241 cgaacgcttt cattgctgct gcgccattct tcggatcggt gtaatagaaa ccgatcagca
     6301 gataggagca caggcccacg ccttcccagc cgaggtacat cagcagcagg ttatcggcga
     6361 gaaccagaac taccatgctg gcgatgaaca ggttggtgta ggcgaagaag cgagagtagc
     6421 cctcttcacc gcgcatgtac caggaggcgt acatgtgaat aaggaaaccc acaccagtga
     6481 ccaccgagag catggtcagc gacaggccgt ccagcaccag gttaaaaccg atgttaaagt
     6541 cgcctaccga catccacgtc cacagcggct ggctgtatgc ctgctcgccg ttagcgaaga
     6601 aatcaacgcc gataaaggcg gttaccagcg ccgccaggcc cacagagcca acgccgacga
     6661 tcgccgaaac gttttcagac cagcgcccac gggagaacgc cagcaggacg aagccaatca
     6721 atggcaaaat aatggttaag gcaagcatgt tcatccgcgc atctcactta ctgaatcgat
     6781 gttcaggttc tggcgacgac ggtgaagttg cagcagcagc gcaaggccga tactcgcttc
     6841 tgccgccgcg aggctgatgg cgagaatgta catcacctga ccgtcggtct gaccccagta
     6901 gcttccggcg accacaaagg ccagcgcgga ggcgttaatc atgatttcca gaccaatcag
     6961 cataaacagc agattgcgac ggataaccag accggttaag ccaagaacga ataagattgc
     7021 cgcgaggatc agtccatgtt gtaaggggat catgcgtgct cctccgtttt tcttttcgcg
     7081 ctgtcgtctt tacgattgct cagcacttca cccgcacgct cttcacgacc gacgtggaag
     7141 gccacaacca gacccgcgag cagcagcata gaagccagtt ccaccgccag tacgtaaggc
     7201 ccgaacagcg taatacccac tgctttagca ctgattggcg taccgtcgat accctgatcg
     7261 ttaacaccga ggatggcgta aacaatcacc accagcatga tggccgacaa aattgccggg
     7321 ccaatccaca cctgcggttt cagccactgg cgttcctgtt cgatttctga accgcccagg
     7381 ttgagcatca tcaccacgaa cacgaacagc accataatgg cacccgcgta gacgataatt
     7441 tccagcgcac cggcgaagta agcgcctagt gagaagaaca ccccggaaat cgccagcagc
     7501 gaaataatca ggtacagcag tgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc
     7561 gcaagtatgg ctatcaggcc acagatataa aaagcgaact ccattgcccc tctccttacg
     7621 gtaacaggct cttgacgtcg ataggcttgg cttcgttctc tgcttcgccc ttatctttgc
     7681 cgtcgattgc catacctgcc atccggtaga agttatattc cgggtatttg cccggaccgg
     7741 agatcagcag atcctctttc tcgtaaacca gatcctggcg cttgtattcc cccatttcga
     7801 aatccggggt taactgaatc gccgtggtcg gacaggcttc ttcgcacaga ccacagaaaa
     7861 tgcagcgtga gaagttaatg cggaaaaatt ccggatacca gcgaccgtct ttggtttctg
     7921 ctttttgcag cgagatacag ccgaccgggc aggctaccgc acagaggtta caggctacgc
     7981 aacgctcttc gccgtccggg tcgcgggtca gaacgatacg accacgataa cggggcggca
     8041 gatagaccgg ctcttccggg tacattcgcg tttcgcgttt ggcgaacgcg tgcaggccga
     8101 tcatccagat actacgaacc tgggtgccga aacctactaa caattctttt aaggtcatgg
     8161 tcttattgcc ccttattgcg cctgccagag aatgacagcc gccgttacca gcaagttgat
     8221 cagcgtcagc ggcaggcaga ttttccagcc gaaggacatt acccggtcat aacgcggacg
     8281 cggtaacgac gcacgaatca aaatgaacat catcataaag aacgcggttt tcagcgcgaa
     8341 ccagatgaat ggcggtaaca acgggccttg ccagccaccg aagaacagcg tcaccatcaa
     8401 tgcagagatg gtcacaatcc cgatgtactc acccacgaag aacagaccga acttcatacc
     8461 ggaatattca atgtggtaac catccgccag ttcctgctcg gcttccggct ggtcaaacgg
     8521 gtgacggtga cacaccgcca cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg
     8581 gatgacgttc cacacatgcg cctggctgtt gacgatgtcg gtcatgttga atgaaccggc
     8641 ctgcgccacc acgcccatca gggaaagccc gaggaacact tcgtagctca gggtctgcgc
     8701 agaagcacgc atcgcaccca gcagcgagta tttgttgtta cttgaccagc ccgcaaacag
     8761 caccgcgtaa accgccagac ctgccatcat caggaagaac aaaatcccga tgttcaggtc
     8821 ggcaaccacc caacccggac tgactggcac aattgcaaag gccagcagca gcgaggtaaa
     8881 ggcaatcatc ggtgccaggg taaagatgac gcgatccgag aatttcggga tccagtcttc
     8941 tttaaagaac attttgatca tgtccgcaac cagctggagc gaaccgcccc agccaacacg
     9001 gttaggtcca taacggttct ggaacagacc cagcaggcga cgttcgccaa agctcatgaa
     9061 tgccccacag gtgacaacca ccagcaggat caccaccgct ttgaggatgg tcagcaggat
     9121 ctcaatcagt tccggtgata tccaactcat tgttgtgcct ccttgagatc ctcaagatgc
     9181 gcgccagcca gcaccggagc aatgccggac atacccatcg gcaagcccac ctgccctgcc
     9241 gtcagtcctt cggcgatttc aaccggcagc gtgaccgtgt tgccatcgta actaaaggag
     9301 acgcgtgtac ctgcgttcac acccaacttc gcggcatccg ctgggttgag tttgatgtac
     9361 ggctgcggca tacggctctg gaagaccgga gcacgctgtg acaattcatc gctgccaaac
     9421 aggtggtagt acggcgcgat acgccatttc ccgtcctgcg gctggaagcg tgccggtacg
     9481 ctggtgaagt aatccagacc gttttcgctg gtttcaaaca gacgcacgcc cggatcgcca
     9541 aagcgcagtt tgccgcccac ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag
     9601 cccggcgccc aggcaaacgg cacttgcgaa cggtgcgcgg tcggctggtt gttaccttcc
     9661 atcgagaagg tgaacatggt gtcaatatcc tgcggctgac gcggctcatg aacgctgata
     9721 ttggcgcgca tggcggtacg accgctgtaa cggtgcggtt cacgggccag tttctgccca
     9781 cgaatacgga atgtcgcatc cggcgcagca tctttgatac ccgccagttc tgggattttc
     9841 gccacaacag cgtcaattac atggtcgagc tgcgtccagt ccacttcacg gctcagcaag
     9901 gtgctgtgca gcgagtgcag ccagcgccag ctttccagca tgacagtttt gctgtcgtaa
     9961 taagccggat cgtaaacctg gaagaaacgt tgggcgcgac cttcattatt aattaccgta
    10021 ccgtcgcttt cagcaaagct ggcagaggaa agcaccagat gggcgttttc catgatcgct
    10081 gtgcgttgat gatcaaccac catcaccagc ggcgctttag ccagcgcagc attcacgcgg
    10141 gtagcagaag cgtaacgatg cagatcgttt tccaacacca ccaccgcgtc ggcgcgtccg
    10201 gtttccagtt cggttaacgc ttcttcaagc gaaccgccac ccataatgcc cagccccatg
    10261 ctgttgacgg aacgggcaat catggtgata ccaacgtcag cgccgcgacc tttcagggct
    10321 ttcgcgacgt tagccgcagc ctgaatcacc tctatgctac cggcgttcgt cccggagata
    10381 atcaacggtt tcttcgcacc tgccagtgcc tgcacgatga cgtcgatttt gttttgcagc
    10441 tcaggttcga taccgtcaac cgctggtgcg gagttatcca gcgcatgggc gatggcaaaa
    10501 cctaaacgcg cctgatcttc aaccggtgcg cggtaagtcc acgccgcgat atcatccaga
    10561 cgagtgtcat caacgttggt aacaaacagc ggatgcttcg cacgttgacc gatgttgagg
    10621 attgccgcaa tctgccagtc agccactttc tgtgctgcag ccatttcgcg cgctttacct
    10681 ttcaccgcct gacgcactgc cagtgcgacg cgcgcgccgg tctgggtaac gtcttcgccc
    10741 agcaccagta ccgcatcgta agattcgatt tcgcgcagag ccggagtata aatgccgcct
    10801 tcacgcagca ctttcagcgc cagttgcaga cgttcctgct caccgtgagc gataccggtg
    10861 tagaagtttt cttcgcccac cagttcacgc agcgcaaagt tgctttccac gctggcacgc
    10921 ggagaaccaa taccgatcac tttcttcgac tgacgcagaa tatctgccgc gccctgcatt
    10981 gcctgttcgg cgttgagggt aatgaaatca tcgccacgac gctgtactgg ctgacgcgga
    11041 cgatccttca ggttgacgta accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg
    11101 tttaccgtac cgttgtaacg gttttcgata cgacgcagtt cgccgtaacg ttcaccgggg
    11161 ctgatgttac agccgatgga acattgctgg cagatgctcg gcgcaaactg catatcccat
    11221 ttacggttgt aacgctcgga gtgcgttttg tcggtaaata cgccggtcgg gcaaatttcg
    11281 accaggttac cggaaaattc gctttccagc gtgccgtctt ccgggcgacc gaagtagacg
    11341 ttgtcgtgcg cgccataaac gcccagatct gtaccgtcag cgtaatcttt gtagtaacgc
    11401 acacagcggt agcaggcgat gcagcggttc atttcgtgag agatgaatgg ccccaaatcc
    11461 tgattacggt gggtacgttt ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcacg
    11521 gtcatatcct gaagatggca gttaccgcct tcttcacata ccggacagtc gtgcgggtgg
    11581 ttggtcatta accactcgac cacactttca cggaactgtt tcgcttcttc gtcgtcaatg
    11641 gaaataaagg tgccatcgga agccggtgtc atacaggaca tcaccaggcg accacgcgtg
    11701 tcttccgcgt tttggtattg cttcaccgca cactggcggc aagcaccgac gcttcccagc
    11761 gccggatgcc agcaaaagta aggaatatca aggcccagag acagacaagc ttccagcagg
    11821 ttgtccgctc cgttgacctc gtattctttg ccgtctacat gaattgtagc cattagcatg
    11881 cttccagttt tctcggtcag ggaccgagcg ttaatcgaaa ttcggttacc agcgctcttt
    11941 cagcaggttc ggctgaatcc cattaatcaa atgggtattg ctgaacggct gtttgattcc
    12001 cgcctcaaat tcttcgcgga aatatttgat ggcgctctgt aacggctcca ctgcaccagg
    12061 tgcgtgggca cagaaagttt tacccgggcc taagaatcga cacagttgct caagtgtttc
    12121 gatatcgccc ggctggcctt cgccacgctc cagcgcacgc agaattttca cgctccacgg
    12181 cagaccgtcg cggcacggcg tacaccagcc gcaggactca cgggcgaaaa actcttccag
    12241 gttacgcacc agcgacacca tgttgatctc atggtcaacc gccatcgcca gcgccgtacc
    12301 cagacggctg cccgctttac cgatactttc gaattccatc ggcagatcaa ggtgcgcttc
    12361 ggtcaggaag tcagtccccg cgccgcctgg ctgccaggct ttaaatttca gaccatcacg
    12421 cataccaccg gcgtaatctt cgaggatctc gcgtgcggtg gtgccgaacg gcagttccca
    12481 cagtcccgga tttttcaccc gaccggagaa gcccatcagc ttggtgccag catctttact
    12541 tttcgagatg ttctgatacc actccacgcc gttagcgagg atcgccggaa cgttacacag
    12601 ggtttcgacg ttgttgacac aggtcggttt gccccatacg ccggaggttg ccgggaaggg
    12661 gggtttcgag cgtgggttag cacgacgccc ttccagggag ttgattaacg ctgtttcttc
    12721 cccgcagatg tagcgccctg ccccggtatg gacgaacagt tcgaagtcga aacctgttcc
    12781 cataatgttt ttgccaagca agcccgcttc agtggcttcg gcaatggcgc ggcgcagatt
    12841 tactgccgct tcgatatatt cgccacgcag gaagatgtag ccacggtaag ctttcagcgc
    12901 aaacgcggag atgagcatac cttccaccag caggtgcggc agttgctcca tcaacaggcg
    12961 gtctttatag gtgcccggct ccatttcatc ggcgttacac agcaggtaac ggatgttcat
    13021 ggattcgtct ttcggcatca ggctccactt caggccagtc gaaaagcccg cgccgccgcg
    13081 ccctttcaga ccagcgtctt ttacctgatt aacgatttcg tccggagaca gcccggtcag
    13141 cgccttacgc gccccttcgt aaccgttttt gctgcggtat tcgtccagcc acactggctg
    13201 tttgtcatcg cgcagacgcc aggtcagcgg atgcgtttcg ggagtacgga taatgttttt
    13261 catttatacc gctccagcag ttcagggatc gcttccgggg tcagatgcgc gtgagtgtcc
    13321 tcatcgatca tcatgtttgg ccctttatca cagttcccca ggcagcaagt tggcagcagc
    13381 gtaaagcggc catcaaatgt cgtttgccct ggtttgatgt tcagcttttt ctcgagcgcc
    13441 gcctgaatac cctgataccc gttgatatga cagaccacgc tgtcgcaata acggatcaca
    13501 tggcgaccaa ccggctggcg gaagatctga ctgtagaacg ttgccacacc ttcgacgtcg
    13561 cttgccggaa tacccagcac atcggcgatc gcgtggatcg caccatccgg cacccagcca
    13621 cgctgcttct gaacgatttt cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg
    13681 tgcatctcgt gctcaatcgc ttcacgctct gccgcactca gctcaaaagc ctcggtttgt
    13741 ggttgttgat tctcgtgcat aattagcggt ccacatctga cataacaaaa tcgatactgc
    13801 ccagataaac aatcaggtca gacaccaggc tgccgcggat cgccgccgga atttgctgca
    13861 aatgcgcata actcggcgtg cggatacgag tgcgataact catggtgcta ccgtcgctgg
    13921 tcaggtagta actgttgatc cccttggtcg cctcaatcat ctggaaagat tcattggcag
    13981 gcatcaccgg cccccacgac acttgcagga agtgggtgat cagggtttcg atatgttgca
    14041 gcgtgcgctc tttcggcggc ggcgtggtca gcgggtgatc cgctttgaac gggccttccg
    14101 gcatgttgtt gaggcactgc tcaagaatgc gcagactctg gcgcagctct tccactttca
    14161 gcattacgcg ggtgtagcag tcagaaacac caccaccaac cgggatttca aagtcgaagt
    14221 tttcatagcc agaataagga cgcgccttac gcacgtcgaa gtcgatcccg gtagcacgca
    14281 ggcccgcgcc agtggtgccc cactccagcg cctctttcgc gccataggcg gcaacgccct
    14341 gggaacgacc tttcagaatg gtattttgca gcgccgcttt ctcgtaagac gccagacgtt
    14401 tcggcatcca gtcgaggaac tcacgcagca gacgatccca gccgcgcggc aggtcgtgcg
    14461 ctacgccgcc aatacggaac cacgccgggt gcatacggaa acccgtgatc gcttccacca
    14521 gatcgtaaat tttctgacga tcggtaaagg cgaagaacac gggcgtcatt gcgccgacgt
    14581 cctgaataaa ggtcgagatg tacagcaggt ggctgttgat acggaacagt tcggagagca
    14641 taacgcgaat gacgttaacg cgatccggca cggtgatccc ggccagtttc tctaccgcca
    14701 gcacgtaagg catttcgtta acgcagccgc cgaggtattc aatacggtcg gtatacggaa
    14761 tgtagctgtg ccaggactgg cgctcgccca ttttctccgc accacggtgg tggtagccga
    14821 tgtctggtac gcagtcgaca atctcttcgc cgtcgagttg taaaacgata cggaaagccc
    14881 cgtgcgccga cgggtggttc ggaccgaggt tgaggaacat gaagtcctca ttttcggtgc
    14941 cgcgtttcat cccccactct tccggtttga aggtcagggc ttccatctcc agatcctgtt
    15001 tggctttggt cagctcaaac ggcgagaatt cggtagcgcg tgccgggtaa tctttacgca
    15061 gcgggtgacc tttccaggtt tgcggcatca tgatgcggcg caggttcggg tgaccgtcga
    15121 aagtaatgcc aaacagatcc caggtttcac gctcatacca gttagcgttc gggaacagtt
    15181 tggtgaaggt cggtacgtgc aggtcgtttt ctgccagcgc caccttcagc atgatgtcgc
    15241 ggttacgatc gatagaaatc agatggtaga aaacggaaaa atccgcggca ggtaaccctt
    15301 cgcggtgtgt gcgcagacgt tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt
    15361 tcggcagttt ctttaagaaa tcgccaactt ccagtaattg ttcacgcttg atccacacaa
    15421 cgggaacccc ggtgcgagtc gcctgaacag taaaggcatc cggcccaaaa cggttgcgca
    15481 gttcgccaat taccggatca tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg
    15541 ttaagtcggt catattgttc accattgcaa atggtccgtg gtgactgtcg gcgcagtaag
    15601 cttcgcgaaa tagatttagg aataagcgaa gaaaaatccc tttgccgaca ggcgcaaatt
    15661 aaatctcgtc aggtgtacgc aggttggtta cggcaatgcg ttcaccgcgc ttgcgttcgc
    15721 gctctgattg catattggcg cgataaacgc cctgatcgcc aaccacccag gagagcggac
    15781 gacgttcttt gccgatagat tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac
    15841 gcggcgggca gcccgggata tacacatcaa ccgggatgaa tttatcgacg ccctgcacaa
    15901 cggaataaat atcgtacata ccaccagagt tggcacaggc acccattgag ataacccatt
    15961 ttggttccag catctggtca tacagacgct gaataaccgg tgccattttg gtaaagcagg
    16021 ttcctgcaac caccatcagg tcagcctgac gcggcgaagc acgcaatact tctgcgccaa
    16081 aacgcgccac gtcatgcacc gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa
    16141 gaccgaagtt atacggccaa attgagtttt tacgacccca gttaaccatg tcattgagct
    16201 tgcccataaa cacgtttttg ttaacttctt gctccagagg gtcggttacg atctcctgct
    16261 tttgcagggg gtaacggtcg ttctcaccgt tgggatctat gcgggtgagc gtataatcca
    16321 tcttaatgcc tcgcggttag cgttgacgat tagcgatact gttcgtttcc gggttcatac
    16381 gctcgcggcg tgaacgcgcg ggcgtccagt ccagcgcgcc aatacgcacc agataaacca
    16441 gacctgccag taacacaaaa ataaaaattg cagcttccac aaagcctacc cagccgcttt
    16501 cgcggataga ggttgaccat gcgaacagat acagcgcttc aacgtcgaag ataacgaaga
    16561 acatggccac cagataaaac ttggcggaca ggcgtaagcg ggcggagccg accgagtcga
    16621 taccggattc aaacggcacg tttttcgacc tcgcgcgtgc gcgaccgcct aaaaaccaac
    16681 cgcctaccag catcaggcaa cacaggccaa tggcaacgat aagaaagata gcgaatgccc
    16741 agtgatgagc gatgacttca gtggatgttg acatactcat tgcttactca tcaaaagtag
    16801 cgccagattc actgctcttc acggcagatg gacgccacat cgattcatgg ggaggaataa
    16861 aaaaaacctt acaataactg tagaaattct tttatacagc taattgatgt ggtcttttac
    16921 tcctttctat aaccttttgt caactttaac aaaagcttct tcacattagt ttacataata
    16981 tcaacaccat tagcatttaa tgccctttca ccccagatcc ttgacgactc caggataatt
    17041 agatgttgtt gaatcgtgtc cgttgtgaag caatggaaaa aatacgggtc tattttgaca
    17101 ggaatttgtg tcgattcctc cccccaaaag agagtatttt cttgatctgt gacacgcttt
    17161 tgttattcca taacaaaaac gcagcaacaa atttacgtat tttttaacat cattgtagca
    17221 ggtgattttt ttcaggcgat tatttgtgca ttcgggacgt gaatctctgg tgattgaaaa
    17281 ataaacagtt ttgtacgttc tgcactatgc gaggaaggat ttttactaaa aaaaagccgc
    17341 tggggtttaa aacaccccca gcggctcgtt ttttacacta ttgtctcagg aattatctat
    17401 cgtccgtcga ttactcgata tccctttcaa tcaacaatga atcatcccct tccggagcag
    17461 acattgggct gtattgccac ggattatggt agctctccat cgcctgatag atcacctggg
    17521 ccagctcatt attactggac gggtcatagc acagcaaata ttcggtatca ggtagcggcg
    17581 gtaagccatc tactccactc agcacacgca ggtccgggct catcatctca accggccttg
    17641 ccgtcacgcc aagaccggct ttcactgccg cacgaacggc cggaagcgtc gaggcgacat
    17701 aagccagtcg ccatggaata tctactttat taagcgtcgc cagcaccata tcgcgaaacg
    17761 gacttggatc atccagcaat acaagaggga tcggctcgcc tttttgcaat acgtattccg
    17821 ctgcgcagta ccagtgtgtt ggcgaggtac gcaggttcaa tgccttaaac gctgaaggac
    17881 gatgggtggt taccatcaaa tccacttcct gggattccag catttctgcc atataggcat
    17941 tacgtttaac gcggacatcc agcgctaatt tcggataaac cgaactcacg cgatttaaca
    18001 ggaaaggtaa gatcgtatcg gcagattcat ctgaagcacc gatagttaac acgccctgaa
    18061 gattactgaa cattaatgat gagcaggcct catcattaaa acgcaggatt ttcctggcgt
    18121 aaccaagaag ttgaatgcca tgttcagtta acagtttgtt gcgaccgtga cgagcgaaca
    18181 gttctttccc aacgagttgt tccagacgct gcatttgctg acttacggcg gactgagtac
    18241 gacacacagc ggcagctgcg gcagcaaaag tgttcagatc ggcaacagca acaaatgttc
    18301 tcagcagatc gaggtcgagg ttaattatcg gacgatttgc acttatcata tattatcact
    18361 tactggcggc tcatactgag ctggttaatg ctgtgcacac acaaacaagc aattccattt
    18421 gtaatgtgcc tccctggcag tttcatccgg aacccggacg aaagtaaaaa tgcatatgag
    18481 ttgcactaaa aaagcgactc acattgttcc gttataatgc ctgaagtaga tcacagaata
    18541 tatcttcagg gatcgcatat ctattaagtt actcactctt ttctatttat gacatgcgcg
    18601 tgtttgtata aatgtaaatg tgagtccttg ttccactctc gtgcagcatc gctggtcata
    18661 cgcgaacacg tacaaacagc aatggtgaag cggcatcaag agcagggatc cgtcatttat
    18721 gcgagcattt taccccaaaa accttttatt tataagggtc attgcgaatt atctgatgca
    18781 aagttatgtt atgttaggca aagtaatctt ctattattaa taagcacatc aaaacctttt
    18841 tgaatattaa ataataatta attagcatca tcctcattca ttaattccgc ttaacaatag
    18901 tttcacacct tacccccatt gcgacacctc ggaaatcatc acgctgttaa actctccgta
    18961 atgcggaaat atcatcatcg cgtttcatta gtgaattctt aaatgaggca ttttcacaca
    19021 attatcttac agataaaaaa accagacttg caattaagaa ttagaacaat tcactatata
    19081 acattgcatg taaagcatat aagcctaatt atttgtcatt attaagttat taacagcaca
    19141 atcgagcctt cccctctggc aaaatcttat tctgcagacc ttcaaaacac cgtcctgggg
    19201 gagtacattg ttctaagctg acttccacgg cagggagtgg cgataacagc aaaaaaggtc
    19261 aagattcatg tcccccattg aaaaatccag caaattagag aatgtctgtt atgacatccg
    19321 tggtccggtg ctgaaagaag caaaacgcct ggaagaagaa ggtaacaagg tactgaaact
    19381 gaacatcggc aacccagccc cattcggttt tgacgcgcca gatgaaatcc tcgttgacgt
    19441 gatacgcaac ctgcctaccg ctcaagggta ttgcgattcc aaagggcttt actccgcgcg
    19501 taaagccatc atgcagcact accaggctcg tggcatgcgt gatgttaccg tggaagatat
    19561 ttacatcggc aatggtgtat cggagcttat cgttcaggca atgcaggcat tgctgaacag
    19621 cggggacgaa atgttggttc ctgcaccaga ttacccactc tggaccgcgg cggtttcgct
    19681 ttccagcggt aaagcggtgc attatctttg cgatgaatcc tctgactggt tcccggacct
    19741 cgatgatatt cgcgctaaaa ttacgcctcg tacgcgtggg attgttatta tcaacccaaa
    19801 taacccaacc ggcgcggtat attccaaaga gcttttaatg gagattgtgg agattgcacg
    19861 tcagcataat ctcattatct tcgccgatga aatttatgac aaaattctct acgacgatgc
    19921 tgagcatcac tcaattgcgc cgctggcacc tgacctgctg accattacct ttaacggact
    19981 gtcgaaaacg taccgcgttg caggcttccg tcaggggtgg atggtgttga acgggccgaa
    20041 aaaacacgcc aaaggctaca tcgaaggtct ggaaatgctg gcttcaatgc gcctgtgtgc
    20101 taacgttcct gcgcaacacg ccattcagac cgcgctgggt ggttatcaga gcatcagtga
    20161 atttattacc cctggcggtc gtctttatga gcagcgtaac cgcgcgtggg aactgatcaa
    20221 cgatattccg ggcgtttcct gcgtgaaacc tcgtggtgcg ctgtatatgt tcccgaaaat
    20281 cgacgccaaa cgctttaaca ttcatgacga tcagaaaatg gtgctggatt tcctgttgca
    20341 ggaaaaagtt ctgttggtgc aagggacggc attcaactgg ccgtggccgg atcacttccg
    20401 cattgtcacg ctaccgcgtg tcgatgatat cgagctgtct ttgagcaagt tcgcgcgttt
    20461 cctttctggt tatcatcagc tgtaatctta atttcactgc cggagattgc atccggcagt
    20521 gttatcccgc cacaatgacc tgatgatgtc atcatacgta aggtcactat gaaacagagc
    20581 catttttttg cccatctctc ccgcctgaaa ctcattaacc gctggccgct aatgcgcaac
    20641 gtgcggacgg aaaatgtgtc cgaacacagt ttgcaggtag cgatggtcgc ccatgcgctg
    20701 gcagctatca aaaatcggaa atttggcggt aatgtcaacg ccgaacgtat cgctttactg
    20761 gcgatgtacc acgatgcctc agaagtgctc accggcgatc tccctactcc ggtgaaatac
    20821 ttcaatgcgc aaatcgctca ggagtacaag gctattgaaa aaatcgctca gcagaaactg
    20881 gtcgatatgg ttccggaaga actgcgggat atctttgcgc cgttaattga cgagcatgca
    20941 tatagcgatg aagaaaaatc gctggtgaaa caggcagatg cactgtgtgc atatctgaaa
    21001 tgtctggaag aactcgcggc cggaaataat gaattcttgc tggcaaaaac gcgactggaa
    21061 gcgacgcttg aagcgcgtcg cagccaggag atggactact tcatggaagt atttgttccc
    21121 agcttccatc tttcgctcga tgagattagc caggattcac cgctgtaagc agccggagtc
    21181 tgcgtcgcat cagtcaataa gcgccggatg cgacatcagg ctcttgtcaa aacggaaaca
    21241 gcatcgggat catcaccaca caaaccgcca tcacgataat ggtgaacggt acccccaact
    21301 tcacaaagtc actgaagctg taatttcccg gacctaaaac cagggtgtta acaggtgaag
    21361 acaccggtgt cataaaggcg gcggatgctg ccatcgccac gaccatcgca aatggataag
    21421 gcgacactcc catcgttttg gcagcagcca gcgcaatcgg agccatcaac accgccgtcg
    21481 cggtattaga gataaacagc ccaataaccg ccgacaagac aaacaaacac cccagcatca
    21541 tatgtggccc ataaccgccg ccaatatcca tcagcccttt caccgccagc gcgacacctc
    21601 ccgttttctg taatgccaca gcaaacggca tcatcccaac gatcaaaata atgctcggcc
    21661 agtgaatgga tttataggcg ctttcagcat ctatacagcg gaatttcccc atcagcaggc
    21721 aggcgatgat agcggcaaca ggattaggaa tttcatttgt cagcattaac gccaccatta
    21781 gcaccagaca gaaaatggca tggggtgcct ggctgtgcgc gggcgatgct tcactcacct
    21841 cttccggtaa gttcagcgct acgaagtcgc ggccctgttt ggccagcata ccgatcagtt
    21901 tccagttacc cacaaccagg atgatatcgc ccagcagcag aggctcatcc gccagcgaac
    21961 cttccagcgc cacgccattg cgcttgagcc ccaccacatt cagtccgtag cgggtacgaa
    22021 aaccaatttc gcgcaccgat ttaccaatca gttctgactc aggaattaat gaaatctctg
    22081 ccatgcccac atcaagggcc tggtcagaaa aatactcgcc gcgcagtacc atcggctcca
    22141 gcaattgctc actacaaaat tgccggagat cgacatcagc cgcagacata tcaataagca
    22201 aaacgtcacg cgcgcgaaat tcagaaaccc cattaacgtt cacgataacg cgacgaaaac
    22261 gccgccagcg ttcaacaccg atgacgttag cgccataacg ctcacgtaat ttgagatcat
    22321 ccagccgttg acctatcatt ggcgatccgg ggcgaatagc cagacgtcgc gcacgcccgg
    22381 tcagtcgata ttcacggata agatcgcgaa aggttcgacg cgtccagcct tcgcgctgcg
    22441 gggtctgggt atcccctttc agcatgaaac gcatcactaa catatacaaa atacccagca
    22501 ccagcacgac caggccaata ggtgttacgc taaagaaact gaagccgtga tagccttcac
    22561 gcagcaattc actgttgact accaggttcg gcggcgtcgc caccagcgtc atcatgccgc
    22621 taatcagccc ggcaaaactt aacggcatca tcagacgtga cggcgaagtt tgcatacgca
    22681 tggcaacgct taacaccacg ggaataaaga tagcgacaac gctggttgaa ctcataaacg
    22741 cgccaagccc cgcgacggtc agcatcaaca aaaccaacat tttgatttca ctgttgcccg
    22801 ccactttgac cagccatgtt cccattacgg tggcaacacc ggtacggacc aaaccatcgc
    22861 caataataaa caaggcggca atcaggacaa cgttaggatc agaaaagccg gaaaatactt
    22921 cggggaccgt cagcgttccg cttaatgcaa acgcgacaat aacaaacaaa gcgaccgcat
    22981 ccatacgcac tctgcccgtc gcaaacaaga cgatggcaac ggccagtaat gaaagaaccc
    23041 aaatcaattc accgttcaca acttatcctt gttaattgag ggggatgact tgattctgcc
    23101 ataaaaaagc cccgacgaga cggggctaaa tcatgatcag gtgtttcact gaataataac
    23161 atcgccattt ggctgtttgg tcacagtaat ttgctccaga ctgtggagga ccaaatcgac
    23221 ctcattcagg cgcggggtat ctgccggagc gttaaccgca atgacatggc aacccgccgc
    23281 caggccagaa agcacgccag cgggagcatc ttccaccacc acacactcct gcggcgcaag
    23341 ccccagcagc tgcgcaccta acagatacgc atcaggttct ggttttccgc gcttcactcg
    23401 ctcagcggtt acaaacacct ctggtgcggg aagccccgct attttatggc gcgctcgcgc
    23461 taccggcatg gagccagaag tcacaatggc ccacggaata cctgctttat tcaaatgatt
    23521 gagtaaggcg attgccccag gaagcgcggt aataccttcg gtttccgtgg cctcgatttg
    23581 ctccagacgc gtaaactcgg cggcaatatc agcctcggat ttgcccgcca taaaatggcg
    23641 caaagaggtg atcgcctgtt taccgtgaat gaaagccagc acctcttccg gcgctaaccc
    23701 atgacgtctg gcccagttgc tccacgcccg ttctaccgca ggcagggaat ccaccagcgt
    23761 tccatcaaga tcaaacagaa aacctttgca ccgcacgcgg gcctcctcag gcgttgataa
    23821 tttgattaat ttcgttggcg ctcaaatggt actgacgcgg gcaggcatgc cacacattaa
    23881 gcatgcgctg atatttttcc cacattggcg tctgggcgtt aaaaccatga gtcccagcgt
    23941 caaaatgggt atagcgccct tccacattaa ccataaagcg gacataaccg aggtaacgtg
    24001 cttcagtggc ggcgtcaaag ccgaggaagg tgacacgacg ttcatcgatg gattgctggt
    24061 cctgcaaatt agaccaagaa acatacaacg catgatacat atccataatg tcgatgatag
    24121 tgcggcaggt ttcttctttc agctcgccaa actcgcgatc caattcacgc atctgtaatc
    24181 cgtaaccacg ctcaataatt gtttgcaggc gacggtaacg ttcagcattt gccggatcga
    24241 gcatagtcat catcttgtac tgattagaca aaataagacg ttgcgcgttg gtcatttcca
    24301 ttgttgactc ctgtatcact ctactacggt gaaaaaaaag aaggctgagt atgccttctt
    24361 ttatatgcgt aatcaggggt caattacaaa tcatcaagga aagttttatc cagttgtttg
    24421 aaggcgcgct taagcgtgtc agctaatgcc tggtaatcag gcttgccttc aacgggtgcc
    24481 aacacctgtc cagactcctg caatttaccg cgaacttcat aaaaccagtt aaggattgca
    24541 gggggtaatg gcgttacaga acgcttgccc agccaccaca atccctgcat gggtaaactt
    24601 aaggcgaaca gtgcagtggc aactgccggc ccaagcagac cgcccagggc aatctgccag
    24661 cagagagtaa atacggcgat cggcggcata aaacggatcg cataacgcgt catcttgata
    24721 acgcgatttt cgacaaagac cggagcaagg cgtttttcca gtggccacgt ctttgagtaa
    24781 tgctgtccgc ggcgaaacaa gctaaaaaaa ttaacagaac gattatccgg cgttgacatg
    24841 cttcacctca acttcacata taaagattca aaaatttgtg caaattcaca actcagcggg
    24901 acaacgttca aaacattttg tcttccatac ccactatcag gtatccttta gcagcctgaa
    24961 ggcctaagta gtacatattc attgagtcgt caaattcata tacattatgc cattggctga
    25021 aaattacgca aaatggcata gactcaagat atttcttcca tcatgcaaaa aaaatttgca
    25081 gtgcatgatg ttaatcataa atgtcggtgt catcatgcgc tacgctctat ggctccctga
    25141 cgttttttta gccacgtatc aattataggt acttccatgt cgagtaagtt agtactggtt
    25201 ctgaactgcg gtagttcttc actgaaattt gccatcatcg atgcagtaaa tggtgaagag
    25261 tacctttctg gtttagccga atgtttccac ctgcccgaag cacgtatcaa atggaaaatg
    25321 gacggcaata aacaggaagc ggctttaggt gcaggcgccg ctcacagcga agcgctcaac
    25381 tttatcgtta atactattct ggcacaaaaa ccagaactgt ctgcgcagct gactgctatc
    25441 ggtcaccgta tcgtacacgg cggcgaaaag tataccagct ccgtagtgat cgatgagtct
    25501 gttattcagg gtatcaaaga tgcagcttct tttgcaccgc tgcacaaccc ggctcacctg
    25561 atcggtatcg aagaagctct gaaatctttc ccacagctga aagacaaaaa cgttgctgta
    25621 tttgacaccg cgttccacca gactatgccg gaagagtctt acctctacgc cctgccgtac
    25681 aacctgtaca aagagcacgg catccgtcgt tacggcgcgc acggcaccag ccacttctat
    25741 gtaacccagg aagcggcaaa aatgctgaac aaaccggtag aagaactgaa catcatcacc
    25801 tgccacctgg gcaacggtgg ttccgtttct gctatccgca acggtaaatg cgttgacacc
    25861 tctatgggtc tgaccccgct ggaaggtctg gtcatgggta cccgttctgg tgatatcgat
    25921 ccggcgatca tcttccacct gcacgacacc ctgggcatga gcgttgacgc aatcaacaaa
    25981 ctgctgacca aagagtctgg cctgctgggt ctgaccgaag tgaccagcga ctgccgctat
    26041 gttgaagaca actacgcgac gaaagaagac gcgaagcgcg caatggacgt ttactgccac
    26101 cgcctggcga aatacatcgg tgcctacact gcgctgatgg atggtcgtct ggacgctgtt
    26161 gcattcactg gtggtatcgg tgaaaatgcc gcgatggttc gtgaactgtc tctgggcaaa
    26221 ctgggcgtgc tgggctttga agttgatcat gaacgcaacc tggctgcacg tttcggcaaa
    26281 tctggtttca tcaacaaaga aggtacccgt cctgcggtgg ttatcccaac caacgaagaa
    26341 ctggttatcg cgcaagacgc gagccgcctg actgcctgat ttcacaccgc cagctcagct
    26401 ggcggtgctg ttttgtaacc cgccaaatcg gcggtaacga aagaggataa accgtgtccc
    26461 gtattattat gctgatccct accggaacca gcgtcggtct gaccagcgtc agccttggcg
    26521 tgatccgtgc aatggaacgc aaaggcgttc gtctgagcgt tttcaaacct atcgctcagc
    26581 cgcgtaccgg tggcgatgcg cccgatcaga ctacgactat cgtgcgtgcg aactcttcca
    26641 ccacgacggc cgctgaaccg ctgaaaatga gctacgttga aggtctgctt tccagcaatc
    26701 agaaagatgt gctgatggaa gagatcgtcg caaactacca cgctaacacc aaagacgctg
    26761 aagtcgttct ggttgaaggt ctggtcccga cacgtaagca ccagtttgcc cagtctctga
    26821 actacgaaat cgctaaaacg ctgaatgcgg aaatcgtctt cgttatgtct cagggcactg
    26881 acaccccgga acagctgaaa gagcgtatcg aactgacccg caacagcttc ggtggtgcca
    26941 aaaacaccaa catcaccggc gttatcgtta acaaactgaa cgcaccggtt gatgaacagg
    27001 gtcgtactcg cccggatctg tccgagattt tcgacgactc ttccaaagct aaagtaaaca
    27061 atgttgatcc ggcgaagctg caagaatcta gcccgctgcc ggttctcggc gctgtgccgt
    27121 ggagctttga cctgatcgcg actcgtgcga tcgatatggc tcgccacctg aatgcgacca
    27181 tcatcaacga aggcgacatc aatactcgcc gcgttaaatc cgtcactttc tgcgcacgca
    27241 gcattccgca catgctggag cacttccgtg ccggttctct gctggtgact tccgcagacc
    27301 gtcctgacgt gctggtggcc gcttgcctgg cagccatgaa cggcgtagaa atcggtgccc
    27361 tgctgctgac tggcggctac gaaatggacg cgcgcatttc taaactgtgc gaacgtgctt
    27421 tcgctaccgg cctgccggta tttatggtga acaccaacac ctggcagacc tctctgagcc
    27481 tgcagagctt caacctggaa gttccggttg acgatcacga acgtatcgag aaagttcagg
    27541 aatacgttgc taactacatc aacgctgact ggatcgaatc tctgactgcc acttctgagc
    27601 gcagccgtcg tctgtctccg ccagcgttcc gttatcagct gactgaactg gcgcgcaaag
    27661 cgggcaaacg tatcgttctg ccggaaggtg acgaaccgcg taccgttaaa gcagccgcta
    27721 tctgtgctga acgtggtatc gcaacttgcg tactgctggg taatccggca gagatcaacc
    27781 gtgttgcagc gtctcagggt gtagaactag gtgcaggcat tgaaatcgtt gatccagaag
    27841 tggttcgcga aagctatgtt ggtcgtctgg tcgagctgcg taagaacaaa ggcatgaccg
    27901 aaaccgttgc ccgcgaacag ctggaagaca acgtggtgct cggtacgctg atgctggaac
    27961 aggatgaagt tgatggtctg gtttccggtg ctgttcacac taccgcaaac accatccgtc
    28021 cgccgctgca gctgatcaaa actgcaccgg gcagctccct ggtatcttcc gtgttcttca
    28081 tgctgctgcc ggaacaggtt tacgtttacg gtgactgtgc gatcaacccg gatccgaccg
    28141 ctgaacagct ggcagaaatc gcgattcagt ccgctgattc cgctgcggcc ttcggtatcg
    28201 aaccgcgcgt tgctatgctc tcctactcca ccggtacttc tggtgcaggt agcgacgtag
    28261 aaaaagttcg cgaagcaact cgtctggcgc aggaaaaacg tcctgacctg atgatcgacg
    28321 gtccgctgca gtacgacgct gcggtaatgg ctgacgttgc gaaatccaaa gcgccgaact
    28381 ctccggttgc aggtcgcgct accgtgttca tcttcccgga tctgaacacc ggtaacacca
    28441 cctacaaagc ggtacagcgt tctgccgacc tgatctccat cgggccgatg ctgcagggta
    28501 tgcgcaaacc ggttaacgac ctgtcccgtg gcgcactggt tgacgatatc gtctacacca
    28561 tcgcgctgac tgcgattcag tctgcacagc agcagtaatc tcgtcatcat ccacagcttt
    28621 gcgctgcgga tatctgaacc ggaaataatc actatttccg gttttttatt ctcttaattt
    28681 gcattaatcc tttctgatta tcttgcttaa ctgcgctgca tcaatgaatt gcgccatctc
    28741 actttgcata cttaccactt tgttttgtgc aagggaatat ttgcgctatg tccgcaatca
    28801 ctgaatccaa accaacaaga agatgggcaa tgcccgatac gttggtgatt atcttttttg
    28861 ttgctatttt aaccagtctt gccacctggg tagttccggt ggggatgttt gacagtcagg
    28921 aagtgcagta tcaggttgat ggtcaaacaa aaacacgcaa agtcgtagat ccacactcat
    28981 ttcgcattct gactaacgaa gcaggcgaac ctgagtatca ccgcgtacag ctgttcacga
    29041 cgggcgatga acgcccgggc ctgatgaact tcccgtttga agggttaacc tcaggatcga
    29101 aatacgggac agccgttggc atcatcatgt ttatgctggt gattggcggc gcgtttggca
    29161 ttgtgatgcg tacaggaacc attgataacg gtatcctggc gcttattcgc catacccgcg
    29221 ggaatgaaat tctctttatt cctgcgctgt ttattctgtt ttcacttggc ggcgcggtat
    29281 ttggtatggg agaagaggcc gtcgcctttg ccattatcat cgcaccgcta atggtccggc
    29341 tgggctatga cagtattacc accgtcctgg tgacttatat tgccacgcaa atcggttttg
    29401 ccagttcgtg gatgaacccg ttttgtgtgg ttgttgctca ggggattgcc ggcgttccgg
    29461 tgctttctgg ctccgggttg cgcatcgtgg tgtgggttat cgccactctg attggcctga
    29521 tctttaccat ggtgtacgcc tcacgagtga aaaagaatcc tcttctgtca cgcgtgcatg
    29581 agtccgaccg cttctttcgt gaaaagcagg cggatgttga acaacgtccg tttacctttg
    29641 gtgactggct ggtattgatt gtcctgacag ccgtaatggt ctgggtgatt tggggcgtga
    29701 tcgttaatgc ctggtttatt ccagaaattg ccagccagtt cttcaccatg ggtctggtga
    29761 ttggcatcat cggcgttgtt ttccgcctta acggcatgac ggttaatacc atggcttcat
    29821 cctttaccga aggggcgcga atgatgatcg cccctgccct gctggtgggt ttcgccaaag
    29881 ggattttgct gctggttggt aatggtgaag caggtaatgc cagcgtgtta aatactatcc
    29941 tcaacagcat tgccaatgcc attagcggtc tggacaacgc ggtcgcggcc tggtttatgt
    30001 tgctcttcca ggctgtattt aatttcttcg tgacgtccgg ttctggtcag gcggcattaa
    30061 ctatgccgtt actggcaccg cttggcgatc tggtcggtgt taaccgtcag gttaccgtgc
    30121 tggcgttcca gtttggtgat ggcttcagcc acatcattta cccaacctca gcttcgttaa
    30181 tggcaacgct cggtgtgtgc cgggtggact tccgtaactg gctgaaggtg ggcgcgaccc
    30241 tgcttggact gctgtttatt atgtccagcg tcgtagtgat cggcgctcag ttgatgggct
    30301 accactaaaa atgttaagag ccgcaattgc ggctcttttt tcattctgcc gtttcagtct
    30361 ctacggcttc atttttggca ttgcgcttca tccacaacgc cagcgctttc agcgagtctg
    30421 gagtgaactc atcgcagcgt gcggtgattt cttccggcgt cagccagcaa actccactga
    30481 cttcatcttc ctgtagggcg aagggaccgt gagagacgca actgaacaac gcgccccaga
    30541 cacggcaatt tttatcttcg aaatagaact gcccgtgctc ggcaaagggg acaccggcaa
    30601 tgcccaactc ttcttccgct tcgcgacgcg cggattccag cagttgctca tcggcctgaa
    30661 ctacaccgcc tgcggtcgca tctaacatgc cgggtaaaaa gtcttttgtc tcggtacgac
    30721 gctggaccag aattttgccc atgccatcat gcacgacgat gtaagttgca cgatgacgca
    30781 gacactgtgc ccgcatttgt tcccggctgg cttgtgcaat gacttcgttc tcttcattga
    30841 caatatccac ccattcagta cttgccaaac gacgctgttc cattatcagg aaaccttctt
    30901 tttctggcgc tcttacggcg cattttgagt tgtgggtaaa ttacggatta atcgcgacct
    30961 gcgcaatgat actttgatca ttgagtgcga ttacactaag tacgtcatta tccagcatgc
    31021 cataacccgc cggattaccg cctttcggaa tactcaccga gccggggttg aagtggaaaa
    31081 tctccccccg ctgttctgcc actggcagat gggtatgacc gtacaccagc acatcgttct
    31141 ggtttaaagc aggtagattt tccgggccaa aaagatgacc atgcgtcaaa aacagacgtt
    31201 gtttttccag taatacctgt tgccacggcg cggttatcgg gaaatgcagc atcatttgat
    31261 ccacttcgct gtcgcagttg ccacgcacag cgataacctt atgtgccact tcattaagcc
    31321 gttcagcgac tttggctggc gcgtaaccct ccggtaaagc attacgcggg ccgtgattca
    31381 acacgtcgcc aaggatcacc agccactggg caccgctttg gacaaacaac tccacaacac
    31441 gttccgtcgc cggtaacgac ccatgaatgt ccgatgcaaa catcagtttc atcactcact
    31501 cctcgtcgaa aaaacgtaag ctatgatact ggattctgtc gttgctatca gccagattgc
    31561 ttcgccgaaa gtgcaataaa acgctggaca gacgctcgct gccactggaa tgtcgcataa
    31621 tccaccaggc gttctggcac ctcatcgcca tgtagtacca ggcggttaat cattagcgcc
    31681 agatcagtat cagcaatgca ccattcacca aataaattcg gctgacccag tgctaacaaa
    31741 tgttctgcca tcgcgaacag tttctctgca ctggcttttc cctcggccgt tagtggcgct
    31801 tttttcgccc ccgcaaagac aacatccgtc ggacgctctt cgcggatggg catcagatcg
    31861 ctgcgcagcc aggcctgaat ctgtcgtgca cgcgcacgat tttctaaatc aagcggataa
    31921 atacgttccc aggtcggtgg cgcaaatcga tcttccagat actccgcaat ggcagaagat
    31981 tcactcaact caaaatcatc gatttgtaat aacggcacac ggcgtgtctg accgtaacct
    32041 tgccacgtcg gctgcaaatg ttcaccgctg tcgaggtcga tggtcttgat atgaaacgac
    32101 aggccttttt cctgcaacgc cacccaggcg gataacacat aaggggagaa aaagtgggca
    32161 tctgaccaaa gcgtgatagc gggtttactc attatgtcct cggcaataaa gggggctggc
    32221 tatcaaacta tagagccttt ttgtcactgt cacgtgacga atactcacgt aagtcgccgc
    32281 gctttctata cttgtggtgt tcagatgatt aaacaaccgg agctgcaatg atcgatctct
    32341 atttcgcccc gacacccaat ggtcacaaaa ttacgctgtt tctcgaagaa gcagagcttg
    32401 attatcgctt gattaaggta gacttgggga aaggcggtca gtttcgtccg gaatttttgc
    32461 gcatttcgcc caacaacaaa attccggcaa ttgttgatca ttctcctgcc gatggcggcg
    32521 aaccgttaag cctctttgag gctgctgcca ttttgttgta tctggctgag aaaacaggac
    32581 tctttttgag tcatgaaacg cgtgagcgcg ccgccacatt acagtggtta ttctggcagg
    32641 taggcggact ggggccgatg cttgggcaaa atcatcattt taatcacgca gccccccaaa
    32701 ccattcctta cgctattgaa cgttatcagg ttgaaactca gcgtctttac catgtactaa
    32761 acaagcggct ggaaaactcg ccctggctgg gaggcgagaa ctacagcatt gcggatattg
    32821 cctgctggcc gtgggttaat gcctggactc gcctgcgaat tgacctcgca atgtatccgg
    32881 cagtcaagaa ctggcatgag cggatccgtt cgcgccctgc caccgggcag gcactgctaa
    32941 aagcacaact cggtgatgag cgttcggata gttaacagaa acaggttctc gtgtattatt
    33001 tcatcctaag taaaacaacg gagaacctgc aatggcacaa cctgccgcta ttattcgtat
    33061 aaagaacctt cgtttgcgta cgtttatcgg aattaaggaa gaagaaatta acaaccgtca
    33121 ggatattgtt atcaatgtga cgatccacta ccccgccgat aaagcgcgca ctagcgaaga
    33181 tatcaacgat gcgctgaatt atcgcaccgt aacgaaaaac attattcagc atgtagagaa
    33241 taaccgtttc tctttgctgg aaaaattaac tcaggatgtg ctcgatatcg cacgtgaaca
    33301 tcactgggtg acgtatgctg aagtggagat cgataaactg cacgcgctgc gctacgccga
    33361 ttcggtatcc atgaccttaa gctggcagcg ttaatcgcca tatcgggagg ctgcatgaac
    33421 atagtgatca ccggagggac gggattaatt ggtcgccatt tgattccgcg tttgctggag
    33481 ctgggccatc aaattacggt agtgacgcgt aacccgcaga aagccagttc cgttctcggc
    33541 cctcgggtga cactatggca agggcttgcc gatcaaagca acctcaacgg cgttgatgcg
    33601 gtaatcaacc tggccggaga accgattgct gataaacgct ggactcacaa gcaaaaagag
    33661 cgtctctgcc aaagccgctg gaatatcacg caaaaactgg tcgatttgat taatgctagc
    33721 gacacgccgc cgtcggtact catttccggc tcggcaacgg gctattatgg cgacttaggt
    33781 gaagtggtgg ttaccgaaga ggaaccgccg cataacgaat ttactcataa actctgcgcc
    33841 cgctgggaag agattgcctg ccgggcgcaa agtgacaaaa cgcgagtgtg cctgctgcga
    33901 accggcgtgg tactggcacc ggacggcggt attctcggta aaatgctgcc accgtttcgt
    33961 cttggcctgg gcgggccgat tggttccggt cggcagtatc tggcctggat tcatattgat
    34021 gatatggtca acggcattct ctggctgctg gataacgagc tgcgcgggcc atttaatatg
    34081 gtttcgccct acccggtacg caatgaacaa tttgcccatg cgctcggtca tgcgctgcat
    34141 cgcccggcta ttttgcgcgt ccccacaacc gccattcggc tgttaatggg cgaatcttca
    34201 gtactggtat taggtggaca acgcgcgctg cctaaacggc tggaagaagc gggttttgcg
    34261 tttcgctggt acgatttaga agaggcgctg gcggatgtcg ttcgctgatg tggtttacag
    34321 caaacatccg ccagttaact ccctgtgtta caggattagt ggctttgcgc gataagatcg
    34381 tctggtgaaa gtcgggtcac catcataact aactctctgt ctaaacctct atccagcatc
    34441 tcctgagcaa tacgcagggc ttcttcgtgt ttgccctgca ttgcgccttc ttcacgtaat
    34501 ctgtcagcaa tggtcatcag tttctccttt tcttgtggtg cgcgttctgt tatctcacca
    34561 ataaatgcac ggaaacgctg ggcatcccct gtttgtaata cgtaattaaa cagggctttt
    34621 agctgtctgt cattagtgtt ccctgtaact aacagcgaaa caatttggtc gactaatccc
    34681 aacagatcgc gctgacgaat atgtttctga attaactcca acagcgccat tttgcggtgt
    34741 tgcataatct cgtcatccgg caccacggta atatccacca acggaaacgc tgatgaatat
    34801 attttgcggg ctatagcagg ctcggcaaat tcatcaagcc agcagagtga ataaggataa
    34861 ggacttctgc aaccatgata aaacagcatc gggatcacca atggaagctc tttatagccc
    34921 gcatcaagat ggttttgcat tgccgcaatg gaataacgca tcatgcgaaa agccattaat
    34981 tcttccggct tactttggtg ctctatcact acataaatat aacccactcc ctcctgcgtt
    35041 ttcacagacc acaagaggtc ggaataatat tgccgcaggt cttcatcaat aaaactgttt
    35101 ggttctagtt taagcgtcgt taaatcacac agtttgcgca gcggcgcggg aagatgaata
    35161 ttaataaaat tccgcgcggt gtctggatgg cgtaaaaaag atttaaatac cgcatcatgc
    35221 ggcgtggaag ttgtcgatat tgtcatggcg ttccgtcacc cctcaaattt aagatgacgc
    35281 gacaataacc acgattaaca cccaatgcag caggcagttt ttatctttgg acagcgaatt
    35341 cagaagtatt tttagcggac tgcaactttt acatttcagc ggaagctgac tcgcaaattc
    35401 cccttgaaaa tactccggca gcgttagcca ccggagagga aattatttca gcgatccctt
    35461 aaggaactgt tgcagacgag ggctttgcgg gttgccaaat aactgctccg gcgcgccctc
    35521 ttcttctatt ttcccctgat ggaggaaaat gacgtgagta gaaacatggc gagcaaagcc
    35581 catttcgtga gtcactacca ccatggtttt cccctcttct gccagttgct gcataatacg
    35641 taacacttcg cctaccagtt caggatcgag cgccgaggta ggttcatcaa acagtaaaac
    35701 ttctggttcc atcgccagcg cccgcgcgat agaaacacgc tgttgctgac cgccggaaag
    35761 atgcaccgga tatttcccct gcgcacgttc gtctatcccg actttcgcca gatacttcac
    35821 cgcccgctcg cgcgcttcct gcttgctcag gcccaacacc tgaatcggcg cttccatgac
    35881 gttttccaac accgtcatat ggctccagag attgaagtgc tggaatacca tcgtcaggcg
    35941 tgtgcgcagt aagcgcagtt gatttttatc ggcgactttg agttgaccgt ctttgtcgcg
    36001 caccagattg atcgtctggc cattgaccac gatcgatcct tcactcggtt tttcgaggaa
    36061 gttaatgcag cgcagaaagg tacttttccc cgatcccgac gatccgatga tgcttattac
    36121 atctccggca ttcgcttgca gtgagacccc tttcagcact tcgtgttcgc catagcgttt
    36181 gtgcaaatcg ataacgttta atttattctc ggacatcgta ttctcagtgc gttgaagaag
    36241 gtttcacatg ctgcaaccag cgtttttccg ctctgcgaaa gaggctgatc aggacataag
    36301 agatgattaa atagagcacc gcggcaatgc cgaaggcggt aaaaggttga tacgtggcgg
    36361 cgttaatatc gcgggctatt ttcagcagat ccggcacagt ggccgtaaac gccagcgcag
    36421 tagagtgcag catcaggatc acttcgttgc tgtatgccgg taacgcaata cgcagcgcag
    36481 aaggcaaaat aatgcagcga tacattttaa aggtcgagaa gccataggct ctggcggctt
    36541 caatttcccc atgcggaacc gaacggattg ccccagcaaa aatctcggta gtgtaagcgc
    36601 aggtgttaag cgtcagcgcc agcacggtac agttcaggcc actgcggaag aaagcgttaa
    36661 ggaattcggt tcccttaaca atctcaagcg tgtacatgcc ggaatagaac accagcaact
    36721 gaacatacag cggcgtaccg cgaaaaatat aggtaaataa ccagattgga aactggatgt
    36781 atttattact ggagacacga ccaatcgcca gaaacagcgc caggactccg cctatcacta
    36841 ccgacaaaat aagcagccac agagtgatcg ccacaccagt aaagcgataa ccgtcggtcc
    36901 acagcagcgg tttccagtat tcatgtaaga tttcgatcac aggtcagccc tcttcacacc
    36961 cacggagtag cggcgctcaa ggaacagcag cacaccattg gaaacggtgg tgaaaaccag
    37021 gtaaatcacg ccacagacga tggcgaaata gaacggttcc caggtacttt tgcctgccag
    37081 ctgcgtggct ttgaccacat cttccaggcc gagtaacgaa accagtgcgg tagatttgag
    37141 gatcacctgc cagttgttgc caatgcctgg cagggcgtaa cgcatcatcg ccggaaacat
    37201 gatccgccga aacacttgcc cgcgagtaaa accgaacgcc gtcgccgcct ctatatgccc
    37261 tttcggcact gccataaacg cgccacggaa cgtttcggta aaataagcac cgtagataaa
    37321 accgagagtg ataataccag cgaccatcgg atcgatatca atctgcccga cgcccatcgc
    37381 ctccgtcacc atgtttagcg caatctgcaa accatagaaa atcagcagca tcaacaccaa
    37441 gtccggcacg cctcgaatca gcgtggtgta cccttcgaaa atcagccccg aaagccgatt
    37501 ttgcgagagc ttaccgccag cgccaattaa accgatgatt acagcgagca ctacagagct
    37561 gatagccagc tccagcgtga cgagcgcacc ctgtaaaata acacctgaaa acccatacaa
    37621 catgctgcct gtcctgtcgt gtgtggtgaa ttaccgcctc ttgtctccct ccggttaccc
    37681 gaagggagcg ggctttcttt caccgatggc gattaaccac cataaacatc aaaatcgaag
    37741 tactttttcg ctaatttctc gtaagtaccg tcagcgcgca tttcggcaaa ggctttgttc
    37801 agtgcctcgc gcagttcgtt atcttcttta cgcaggccca tgccggtccc tacgccaaac
    37861 agtttttcat ctttaacaga cggaccaccg aatttgtaat ctttaccgac gggttgtttg
    37921 aggaaacctt cgctggcagc gacctcatcc tggaacgcgg catcaatacg tccggcagtc
    37981 aggtcagaat aaatgttgtc ctgcccctga tacgagacga tttcaatgcc ttttggtgcc
    38041 cagtgttcat taccgaacgt ctcctgggtg gtgccctgca atacgcctac ccgtttgcct
    38101 ttcagcgact cgactgtcgg ctgaatgtca gaatttttcg ccaccaccag acgagaatcg
    38161 gcagcgtaca gtttgtcggt gaaggcaatt tcttgctgac gtttttccgt aatggaaagc
    38221 gatgacatga tggcgtcaat cttcttcgct tttaaggacg ggattaacgc atccagcggg
    38281 ttttcgacaa acgtacattg cgtattgatg cgcttgcaca actccttcgc cagctcgata
    38341 tcgaaaccaa ccagttcgcc ttgtgaattt tttgattcaa atggcgcata ggtcgggtcg
    38401 gtaccgatgc ggatgttttg cggaattgca gcaagtgccg cagttgcgct ggagaaggcc
    38461 agaaccagag agagcgatag caccagtttt ttcataatgt cctcaactga cagtcttttt
    38521 atagggaata tttacaggtt tgttacactt atcgtgccat aaaattgacc atcaaggcaa
    38581 aatatcccgc cccgttagac gtattatttt tcattaaaaa tgcttaagta tgcatttcaa
    38641 tgcaccacga tagtgcaaca aacctggcgt gcaccatgat gatcattcca tcaggtacag
    38701 cttcccagcg acgtatcagt caccgtagac attaaagtcg aaatactttt tcgccatctt
    38761 gtcgtaggtg ccgtcctgac gcagctcgcc aagcgcctta ttgaaggcag ccgtcagttc
    38821 agcataatct ttacgtagcc ctacaccggt gccatcaccg aagtattttt tgtcttttac
    38881 tgatgagcca gcaaaagcga aatctttacc agcaggttgc ttgaggaatc cttcgctggc
    38941 agcaacttca tcttgtaacg cagcatccag acgtcctgca gccagatcgg aatagaccaa
    39001 atcctggttg gcataggcca ccacatccac gcctttacta cgccaggtct cgttagcgta
    39061 agcttcctgg gttgatccct gcagcacacc aacatgttta cctttcagtg aatccagcgt
    39121 tggctgaatc ggtgaacctt tggcagcaat caaacgagaa tctgcggcgt acagcttgtc
    39181 ggagaaggca atctcctgct gacgtttatc ggtaatggaa agcgacgaaa taatagcgtc
    39241 gatttttttc gctttcagtg aggggatcag cgcgtcaaag tcactggcaa cccaggtaca
    39301 tttcacctgc atccgtttgc acatctcgtt accgagatcg atatcaaagc caacaaaatc
    39361 acctttagca tctttcgacg agaacagcgc ataggtggta tcggttccga tacgtaccgt
    39421 ctccggtagc gccgcatagc tggaagccgc tgcggagaga ccgactaata aagacagagc
    39481 gagaatcgac ttcttcatac ataaccctca agtgaaaatg gcttttttat gttgtgtatt
    39541 gtgttgtgtg tttgcaggct ctttcatgca ggtcttatgc catcttgacg acaacagtaa
    39601 cattcaacgt taaatatgtt aataagacat tgcattattg tcctgaagtt gaagatagca
    39661 ggtatagcgg ttgaatcgca gcgtttcgat tgaatggcag aaacaaaatg tcgaggattt
    39721 gatcgcggtt gcaaaattgc cctgaaacag ggcaacagcg gagttatgcg ccctgccagc
    39781 gggcaaagag atcttcagga agggttattg caaactggtc aagaacacga ttaaccgtct
    39841 gatttatcac atcatcaagg gattgcgggc gatgataaaa cgccggaacg ggaggcataa
    39901 tcaccgcacc gatttctgcc gcctgagtca ttaaacgcag atggcctaag tgcaatggtg
    39961 tttcacgcac gcagagcacc aacgggcgac gctctttcag caccacatct gccgcacggg
    40021 tcagtaagcc atcagtatag ctatggacaa tgccggacag ggttttgatt gaacagggta
    40081 aaatcaccat ccccagcgtc tggaaagaac cggaagagat gctggcggaa atatcgcgcg
    40141 catcgagcgt gacatcagct aatgcctgca cttcgcgcag ggaaaaatcc gtttcgaggg
    40201 ataaggtctg gcgcgccgcc tggctcatca ccagatgcgt ttcgatatct gtgacatcgc
    40261 gcagaacctg taataagcgc acgccataaa tcgcgccgct ggcaccgctg atgcctacaa
    40321 tgagtcgttt cataaatagt tgccctgtca gacttgcagg cagactttgc agtatttcgg
    40381 cgggagttgc aagtcagggt gccagaccgg caccctcagc gaaggcatca tccttcgtta
    40441 tgcatttcga gattttccac ttcgttctga cgttgcactg ctttggcgtc atcattacgt
    40501 aacgtatcga ggaaatcgag gtagccctga tcaacatctt tggtgacgta gacgccgttg
    40561 aacaccgagc attcaaactg ctggatatcc ggattttcag cgcgaacggc gtcgatcaga
    40621 tcgttcagat cctggaaaat caacccgtca gcaccgatga tctggcgaat ttcatcaact
    40681 tcgcgaccgt gagcgatcag ttccgtggcg ctcggcatat caataccata aacgttcggg
    40741 aagcgaattt ccggtgccgc agaagcgagg tacactttct tcgctccggc ttcgcgtgcc
    40801 atctcgataa tctgctcaga agtggtgcca cggacgatgg agtcgtcgac cagcaggacg
    40861 tttttatcgc ggaactcggc gcggttggca ttcagtttac ggcgcacgga cttacgacgc
    40921 agctgctggc ccggcatgat aaaggtgcgg ccaacatagc ggtttttaac gaagccctgg
    40981 cggtacggtt tgcccagaat acgagcaatt tccagcgcga tatcacacga ggtttctggg
    41041 atcgggatca ccacgtcgat atccagatct tcccattcgc gggcaatttt ctcgcccagt
    41101 ttcgtgccca tattcacacg cgcgctgtaa acggaaattt tgtcgataaa cgagtccggg
    41161 cgggcaaagt atacatactc aaacaggcac ggattgctga ccggattgtc agcacattga
    41221 cgggtaaaca actgcccttc ttcagtgatg taaatcgctt cgcccggcgc gacgtcacgc
    41281 aggaaatcaa agcccagcgt atcgagcgct acgctttcgg aagcgaccat atattctgta
    41341 cggttctcgt caatatcacg ttttcccagt accagcggac gaatcccgtt tggatcgcgg
    41401 aaagcaacca taccgtggcc gataatcatc gccacacagg catacgcgcc gcggattaag
    41461 cggtttgtgg cagcaatggc agcgaaaata ttgtcggctt ccagcgggta gtggcggaag
    41521 ttgtccagct cgctggcgaa gatattaagc agaatttccg agtcggaagt ggtgttgatg
    41581 tggcggcgtt tttcttcaaa cagtttttta cgcaactcgt gagcgttggt cagattgccg
    41641 ttgtgggcaa gcgtaatgcc atacggggag ttaacgtaaa acggctgcgc ttcagaggcg
    41701 ctggagctgc cagccgtagg gtaacgcaca tgaccaatgc ccatattgcc ctgcaaacgc
    41761 tgcatatggc gagcttcaaa tacatcgctc accagcccgt tcgctttacg caaacggaag
    41821 cagttattgg catctatggt gatgatgccg gcggcatcct gaccgcgatg ctgaagcacc
    41881 gttaaggcat cataaatcga ctggttaacc ggcataacac cggcgatacc gacaataccg
    41941 cacatacgtc tttttcctcg ttaagccaca tctcagagca cttacgctct gggcaagaaa
    42001 cttgacgagc tttgcagata atcaaaaaac catctgatga taaaactgaa ttgcgggatc
    42061 agctgtgatt tgctccagtc ttcacttttc gacaccccgg taaaggagtc gagaaagaag
    42121 agaatggcgg caacaatcaa cacaccgcgc aacgcaccga aacagacgcc cagcacccga
    42181 tcggtgcctg acaaccccgt tttctccacc aactggccta tcacgaagtt cacgatagca
    42241 ccaacgatca gggtagcgat aaacagtacc gcgatggcaa tcccatttcg aaccagttcg
    42301 tcttcaaagc ccgtaaacca gactgacagg taagtgtagt aatgactggc aacaaagaaa
    42361 gcacaacccc atgtcaccag cgataacgct tcacgaacaa agccgtggat caggctaacc
    42421 agagaggaaa aagcaatcac cgcgattatg gcgtaatcaa tccagaccat atgtgtccca
    42481 cgattttacg ccctgtcatc ctgttcgggg cgcattctaa cagaaaaaga aaacgtttgc
    42541 gtagggattt ccttcccgcg catcaataaa aatggcgctg aaaaaatatt caacgccatc
    42601 gactttttat gcctttgcgg catcgtgcaa tgcgtgtcgg atgcggcgta aacgccttat
    42661 ccgacctacg gttctaacac tgcccaagcc tgataagacg cgccagcgtc gcatcaggca
    42721 agactgtatt aattcggcgt atagcccatt accacgccac ttaagccaga aagttgcttc
    42781 aactcaccca gcgaaccttt cagcttatct ttcgaggcat ccggcccaac cagaatacgg
    42841 gtaattttac cctgcactgg cgtggatggc gacgtataaa cccgataacc ggcaccgcgc
    42901 agcttaccga caatctcatt cactttatcg gcatttttca gcgcacccag ttgcacaaca
    42961 taggctttac ccgttggcgc tgctttttct tccacgaccg gcttcggttc tggcttcggc
    43021 gcaggtggta cttcaacctt tggtttaggc ggctccaccg gtttcggctt cggtggggcg
    43081 accggtgcag gttccggttc aaactcggtg ttattggctg caatagtggc cggatcgagc
    43141 gacggtgctg cggcatcacc tgcccgcacc tcttccgctg cgccttccgg cggctgcgtc
    43201 ggcaacgcct gggtggcggc tggcatcata tcaggctcat cacgatcgcc cgctttcggc
    43261 accagcggga tagccgcgaa ctcatcctga taatgttttt tctgcccgtc cagcagccct
    43321 ggaagtacaa tcacccccag cgccaccagc acgatcgtgc ccactaaccg attctgaaac
    43381 ttacttgcca ccgcttctcc tcgcgtcaat cacttccatg acatgtgcga ccgtgtggaa
    43441 agaaccacac accagcacgg tgtcttccgc tttagcgtcc gccattgcgg catcccatgc
    43501 ctgcgcaacg ctatcaaatg atttgccgtt acccaaatgc tcaagcagtt gttctgccgt
    43561 ggcaccgcgc ggcccttcca gtggcgcaca ataccagtca tcaaccacgc ttttcaacca
    43621 ggccagagtt ccggcaatat ctttatcatg tagcataccg ataaccgcca gcacgcgccc
    43681 gtttttcggt agcgctttca tacgcccggt gagatattcc gccgcatgtg gattatgcgc
    43741 gacatcaaaa ataacgcgtg gcgactcgct cacaatctgg aaacgtcccg gcaaaattgc
    43801 gctggcaatc ccgtcgcgaa tggcattttc actgacttcc agcccgctgg cacgcagtgc
    43861 cgccagcgcc gttgcggcat tcggttgcgg gacaagcggc aacggcagat tttccagcgt
    43921 gccgtgagca tcgctaaacg cccagtcatg atcggtgacg gaatagttcc actcaacgcc
    43981 ccgacgttgt aacagtgcac ctttttcctg cgccacatca gcaatggtag aaggcatttc
    44041 cggctcaccg acaattgccg gtttttcgct gcggaagatg cctgctttct cgagaccaat
    44101 actttcgcga tctggaccca gccagtcggt atgatccagc gcaatactgg ttactaccgc
    44161 gacatcggca tcgacaatat tggttgcgtc cagacgaccg cccagcccta cttccagaat
    44221 caccacgtca agttgtgcct gcttgaacag ccacaacgcc gacagcgtac cgtactcgaa
    44281 ataggtcagg gaaatatcac cgcgtgccga ttcaatctcc gcaaaagagg cggtgtgggc
    44341 cgattccggc aattcctggc cctgcacacg tacgcgctcg gtataacgca ccagatgagg
    44401 cgaactgtag acgcccactt tgtaccctgc cgccatcaga atcgactcca gcgtacggca
    44461 ggtggtgcct ttgccattcg tacccgcaac ggtaaacaca aatggcgctg gtttcaggac
    44521 gccaagacgc gccgcgacct ggctcacgcg ctcaaggccg agatcgatag ttttactgtg
    44581 caggttttcc agataagaaa gccacgaagc cagaggcgac gcggcttgag gagtgcgttt
    44641 gataatcatg gtatccgctg attcgttacg gtgagaatag caaaagggca gagccagtgg
    44701 ccctgccctt atcagttatc aggcctcagg ttcctgatcc ggtaccgggg gtaccactac
    44761 gccttcacgc ggcgcttcag gattcggcgc tggcagattc atcaacttcg ccagaatgct
    44821 cgccagtttc aggcgcattt ccggacgacg gacgatcatg tcgatcgcgc ctttctcgat
    44881 caggaattca ctgcgctgga atctaggcgg cagtttttcg cgaacggtct gttcgataac
    44941 acgcggaccg gcaaagccga ttaacgcttt aggttcagcg atgttgagat cgcccagcat
    45001 ggcgaaactt gcagaaacac cgcccatcgt cgggtcggtc agcacggaga tgtacggcaa
    45061 gccgcgctcc tgcatttttg ccagtgccgc agaggttttc gccatctgca tcagcgacat
    45121 cagtgcttcc tgcatacgtg cgccaccaga ggcggagaag cagatcagcg ggcagttatc
    45181 ttcctgcgcc tgctcaacgg cacgcacgaa acgtgcaccc acaacagacc ccattgaacc
    45241 gcccataaag gcgaactcga atgccgcagc gacaaccggc attccataca gagtgccttt
    45301 catcaccacc agcgcatctt tttcgccggt ttctttctgc gcagatgcca gacggtcttt
    45361 atacttcttg gagtcacgaa acttcagctc atctttcggc tcaagctcgc tacccagctc
    45421 cacaaggctt ccttcatcta acaggctatg caggcgatta cgcgctgtca tacgcatgtg
    45481 atggtcacac ttcggacaga cctcaagatt acgttccagc tcagcgcggt ataaaacctg
    45541 accgcagcta tcacacttag tccacacccc ttcaggaatg ctcgccttgc gggtgggagt
    45601 aatgttgctt ttaattcgtt caatccagct cattagggac ctttctgtct gaacctggtt
    45661 cgatgccagt tttatctttg gggacgcata atgccatttt tgcccccaac agaccatgaa
    45721 tgttgcacat taaaacataa cagcccgaaa ctttggataa aaaagtggtc gaaccgcgga
    45781 gttacttttt attttgcggc gcgtgccgca gcgcgtttgt gacggattat ttcgatgacg
    45841 cccggcaaaa tagaaaccac aataatcccg acgatcagca gcttaaggtt atcctgaacc
    45901 atcgggattg taccgaagaa atagcctgcg taggtaaaaa gcagtaccca caacagtgcg
    45961 ccgatcacgt tataagcagc gaaatgacgg tacgacatgt ggcccattcc cgcaacaaac
    46021 ggggcgaacg ttctgacgat cggcacaaaa cgggcgagaa taatcgtttt gccgccatgt
    46081 ttctcataaa actgatgggt tttgtcgaga taactgcgac ggaaaatttt cgaattggga
    46141 ttactgaata acttttcacc gaacaaccgc ccgatagtgt aattgaccgc gtcacccaca
    46201 atcgcggcga tcagcatcag cactaccatc atatggacgt tgagatcgtt ggtttccagt
    46261 gatgccaacg ctccggcaac aaacagcaat gaatcacccg gtaaaaacgg cattaccacc
    46321 agaccggttt cacagaacaa aattaagaac aaaatggcat aaacccagac gccgtactcc
    46381 gcgactagct ctgccagatg cacgtcaatg tgcaggataa aatcgatgag gaaataaatc
    46441 aggtccatat ttgcctatgc cttgtactcg tcatctttca ggctgtaact acgttggcta
    46501 cagcctgaaa tgttccgagc attattctcg ttagtccgcc agaaatagcg ggcccattgg
    46561 cggttttgga agatcatacc ggtcagggta atccaccgcg accagataca gcccttccgc
    46621 ttttgccgtt gctgccgcca gcgttctgtc ctttgccgcc agcaactctg ctatccagct
    46681 ctccggctgg ttgtgggcac cgacttccat caggctgccg acaatattcc tgaccatatg
    46741 atgtacaaag gcattcgctt tgatatcaac caccacataa ggaccgtgac gcgtgacatt
    46801 aatatgcata acgttgcgcc acggggttcg ggactggcac tgcaccgcac ggaacgaggt
    46861 gaaatcattc tcgcccagca agcattgcgc agcccgatgc atccgttcag cgtccagcgg
    46921 ttcgtaaaaa tgggttaccc ctttactcag taccgccggg cgcagccgat gattgtagat
    46981 gatgtagcga taacggcgag ccgtggcgct aaatcgggca tgaaaatcat caggtacagc
    47041 tttaacccaa cgcacggcga tgtcaccagg taaattcgca tttacgccca aggtccacgc
    47101 cgcgtctttg cgctgcgcgg ttgtttcgaa atgcaccact tgcccggtac cgtgtacccc
    47161 tgcgtcagta cgcccggcgc agaagacggt gatgggttcg ttcgccacct gggagagcgc
    47221 cttttccagc ttctcctgca cactgcggac ttcattctgc cgttgccagc cgtaatactt
    47281 actgccgtcg tactcaatgc ccagcgcaat tttataaact ggcagttgtt gctggtcgga
    47341 cattagtaca gatactcctg caccagtttc tcggcgattt ttactgccat cagcgcgccg
    47401 ccaaagcgaa cgttatcggc caccgaccag aactggactt gctccggcat accgtagtca
    47461 ttacgcacgc agccaacaga aagatgcggc gtacccgaag catcccctac ctgagtcggg
    47521 aattcgttct cttcagagag cacaatatct tcgccctgag caaacgcatc acgcgcttct
    47581 tctgccgcca gcggacgcag tgcttcaaag ttgaccatct gggcatgacc gtagaatacc
    47641 ggtgcctgga cgacgcttgc cgaaatcatc agcccttcgt cctgcaggat tttgcgtact
    47701 tcgtcaacga tacgacgttc ttcacgcacg ctaccttcgc tatccggcaa caacggcagc
    47761 atattgaacg ccagttgacg accgaagaaa tcttcttcgt caatcggaat gccgttgagc
    47821 aatttcgcac tctgccccgc taacgcatcg accgcttttt tgccctgggc ggaggctgaa
    47881 atcaggctgg taacactgat acgcgacaaa ccgccctgat cgattaacgg tttcagtgcc
    47941 gccagcagct ggctggtcag actgtctggt acggcgatga cattccggtt gcggtaatcg
    48001 gtcagtacaa acgggtttac ttccggcacc accagcggta cgtcgggttc gagagcaaac
    48061 agtccgctgc tgtcgatcac caggcaacct gagttggtcg cttcttcaac ccaggcagcg
    48121 gtagcttctt tgcctgcgac aaaaaatgcc agctgcgcct gcgtccagtc gaattcagcg
    48181 gcatcctgca cggtgattgt tttaccacca aagcgcagtt gttcgcctgc gctttcgtta
    48241 cgtgccagtg cataaatttc cccaaccggg aactgacgtt cagccagcgt ttcaagcagg
    48301 gcttcgccca tagcgccagt tgcgcccagg acggcaatgt tccagccttc agacatggtg
    48361 gtttactcca gaaatagcaa agctccctgc caatgttaca gcagagagca tgaagaagag
    48421 attaacgtgc cggatgatga acggcgttaa aacccagttt acacagcaat gatgccgcac
    48481 tggcgtcatc acaaattaca tacagagatg accattcacg gcgctcaaga tagtttttgc
    48541 gcagtttatc gaactcaccc ggtatcccgg cgactttacg cagcggtgcg tcatcgcggc
    48601 gcacatcata caccaaatgc accagccttt tcagcgtcgg ttgatcgagc ggaccatgca
    48661 gcgtaatgcg gccaaactca ggcgcgggca gcaacgtatc cagcgcaacg tgttgttcat
    48721 gcccaataaa cttgctatag gcttcaaaca cttgcgtcgt gccacgcgct ttaccctcca
    48781 gggtatagcc tgcgatatgc ggcgtgccga tatccacttt tgtcagcaat tccacattaa
    48841 gttccggctc gccttcccag acatccagca ccacgcttaa cttctggcct tcattcaggc
    48901 aggtcagcag cgcggtatta tcgacgactg cgccacggca ggcgttaatc agaatcgctc
    48961 cgggcttcag gctacggatc agtttttcat ccgccagatg tagcgttttg tacggaccat
    49021 ctttaaagag tggcgtatgg aaagtcagaa tatccgcgcg ctggactaac tcatccagcg
    49081 agcggaaatc accctcatcc ccacggtcgg cgcgaggcgg atcgcaaagt aaggttttaa
    49141 tccctaacgc ttccagtcgc gcctgcaatc gacgtccaac gttaccaacg cccacgatcc
    49201 ccaccgtacg gtcgtgcagt gaaaatccat cgcgttcggc aagcatcagc agggaggaga
    49261 aaacatattc caccaccgca atcgcattac agccaggtgc agcggaaaaa ccaattcccg
    49321 cctgcttcaa ccatgcttca tcgacatggt cggtccccgc agtggcagtt ccaacaaatt
    49381 taatgggttt tcctgccagc aaagattcat tcactttcgt gaccgaacgc accatcagcg
    49441 catccgcgtc tgccagttga gcgacgggga ttggacgccc gggaaccgcg atcacctcac
    49501 ccaaacggct aaataagtcg cgggcataag gcatattttc atcaacaagg attttcacgt
    49561 ttgtgttacc tgtatgagac gagagttaac cggacaagta tgccataatc tcgcggccag
    49621 gcatacttgc gaagatttca ggtataagga tacgtaatga tacaacctat ttccggccct
    49681 cctcctgggc agccaccagg tcagggagat aacctgccgt ctggcgcggg caatcagcct
    49741 ttatccagtc agcaacgtac ttcgctggaa agcttaatga cgaaagtgac ctcactgacg
    49801 caacaacaaa gagcagaact gtgggcgggt atcaggcacg atattggtct gtcgggagat
    49861 tcaccgctgc tttcgcgtca cttccctgcc gctgagcata atctggcgca acgtctgctg
    49921 gccgcgcaaa aaagccattc tgcccgccag cttttagcgc aattagggga gtatttacgt
    49981 ctggggaatc atcgtcaggc ggtcacggat tatatccgtc ataactttgg tcagacgccg
    50041 ctgaatcagc tctcaccgga gcaattaaaa accattctca ccctgttgca ggaagggaag
    50101 atggttattc cgcaaccaca gcagcgcgag gcgaccgacc gtcctttatt accggcggag
    50161 cacaatgcgc taaaacagct ggtgaccaaa cttgcggcgg caacggggga acccagcaaa
    50221 cagatctggc aatcgatgct ggaactttcc ggggtgaaag atggcgagtt aattccagcg
    50281 aaactgttta accatctggt gacctggcta caggcgcgtc agacgctaag ccagcaaaat
    50341 acgccgacgc tggaatcact acagatggcg ctaaaacaac ctttagatgc cagtgaactg
    50401 gcggcgttat cggcatatat ccagcaaaaa tatggccttt ctgcgcaatc atcgctttct
    50461 tctgcccagg ccgaggatat tcttaatcag ctttatcaac ggcgggttaa agggattgat
    50521 ccgcgtgata tgcaaccgct gcttaatcct tttccaccga tgatggacac gttgcaaaat
    50581 atggcaacgc gtcccgcgct gtggatactg ttagtcgcga ttatcctgat gctggtctgg
    50641 ctggttcgtt aaccccgacg aaatgacagt atcgtgacaa taatacccag caccgcagag
    50701 atcgccccgg caagaaatac cgaagagtaa ccaaacgtgg tcgccagcat tcccgccagc
    50761 ggcccggaga cgccgagggc gatatcctga aacgcggcgt aaccgcccag tgcggtgccg
    50821 cgaacttgtg aggggacgcg tttaaccacc tccacgccca gcgcaggaaa gataagcgaa
    50881 catccggctc cggttaacgc cgcgcccgct aatgcgaccc atgcacctgg ggcttgccag
    50941 agcagcaaca agcccaccgt ttctacaagc agagagacaa tcgccacttt cacgccgcca
    51001 aaacggtccg gcatccagcc aaacatgacg cgcatcacga caaatgcgcc gccaaacgcg
    51061 gtaagagtaa agcccgccat cgcccatcct ttgctggcaa agtagagcga aacgaaagtc
    51121 ccgataaccg caaaaccaac gccttgtagt gccagcccta accctggttt ccagataagc
    51181 ccgacaacgc tccacagcga tggacgttct cccgccaggg ccggtacttt gcgcactgtg
    51241 ccgttacagg cccacgccag taagggtaat accattgtgg tgatcgccag tgcggcaaaa
    51301 ccgtaatggc tatgaatcaa caggccaagc ggcgcaccaa cagcgagggc accgtaaatc
    51361 gccattccgt tccatgacat cactttgcca gagtgttttg gccctacgat gcctaacccc
    51421 caggtcagag cgcctgtcag taactggctt tcaccaaacc caagaatcaa acgcccgatg
    51481 accaacaggg caaatttgaa cggtgcggag acaggcaaaa tcgccgccag cagcaacgcg
    51541 ccgccagcca gaccacaagc taacatcccc tgaagcgccg aacgttttgc gccatattga
    51601 tcggccagtc gcccggcgta accacgcgtc agcaccgtag ccagaaactg aatcccgacg
    51661 gcaatgccga ccatggtatt gccatagccc agatcatgat gaacaaacag cgggataacc
    51721 ggcaacggca accctacggt catgtaggtg agaaaaaccg caaaagagat gcggaagagc
    51781 gaaaaactgg cagaagatcg tgtttcggtt tggcttacag cagtcatgca ttactccaga
    51841 atgcagcgca aggcgaggag tatccccgtc tcatctctct ggtttcaggg ttacggtgcg
    51901 ttggcaggat ttaacgcgta cgtcttttca gaaggaaatc gacaaagcgg gaagtttgcc
    51961 tagaactggt ggcgattgtc aatgatgtga aaaagggaac catcaggttc ccttttgcgt
    52021 tagtgccgga ggccgcattg gcgcgtaacg tcggatgcga cgctggcgcg tcttatccga
    52081 cctactccgg cctactgcga attaatcttt tcagcttgcg cattaccagc gtggcgttgg
    52141 tgccgccgaa gccgaagctg ttagacataa cagtggtcag ttcgcgatcg gtcgtttcgg
    52201 tcacgatgtt cagacccgca gcctgctcgt ccagctcttc aatgttgatg ctcggggcga
    52261 taaagccgtg ttccagcatc agcagagagt agatagcttc ctgcacaccc gcagcgccca
    52321 gtgagtgacc ggtcatggct ttggttgcag aaatcgccgg gctcttatcg ccgaacactt
    52381 cacggatagc tgccagctct ttcacgtcgc caaccggagt cgaagtaccg tgagagttca
    52441 ggtaatcgat tggggtatca acgccatgca tcgccatctg catacaacgt actgcgcctt
    52501 cgccagacgg agcaaccatg tctgcaccat cagaggttgc gccgtagcca acgatttcag
    52561 catagatgta agcaccacgc gccagcgcgt gttccagctc ttcaaccact accataccgc
    52621 cgccgccagc gataacgaaa ccgtcacggt gagcgtcgta agtacgggag gctttttccg
    52681 gggtgtcgtt gtatttagta gatagcgcac ccatcgcgtc gaattcgcag gccatttccc
    52741 agcacagctc ttcgccgccg ccagcaaaca cgatgtcctg tttgcccagt tggatctgct
    52801 ctactgcgtt accgatacag tgtgcggaag tcgcacacgc ggagctgatg gagtagttaa
    52861 cgccatgaat tttaaacggc gtggcgagac aggcagaaac gccggatgcc atcgctttag
    52921 tgaccacata cgggccaacc gctttcaggc cgcgcgggcc gcgcattgcg tcagcgccga
    52981 acacctggaa acgcggggaa ccgccgccgg aacctgcaat caggccaacg cgcgggttat
    53041 tctggtaagc ttccggagag aggcccgcat cagcgattgc ctgctccata gaaaggaatg
    53101 cataaatgga tgcgtcgctc ataaagcgca caactttgcg gtcaatgagg ccagtggtat
    53161 ccagttttat gttgccccag acgtggctac gcatgccgga atccttcagc tcctgagaga
    53221 aagtgatccc tgaacgtcct tcacgcagag atgccaggac ttcctgctgg ttattaccga
    53281 tgctggaaac aatgcccagg ccagtaatca ctgcacgttt cattcaatac ctctgtaagt
    53341 cgcacataga gtaagtttcg aatgcacaat agcgtacact tgtacgccga acaagtccga
    53401 tcagccattt aatagagata tttgcgcagc cttacacaca tcgctaagat cgagccaccg
    53461 cctgtaagac gagtaactta cgtgaaacac tactccatac aacctgccaa cctcgaattt
    53521 aatgctgagg gtacacctgt ttcccgagat tttgacgatg tctatttttc caacgataac
    53581 gggctggaag agacgcgtta tgtttttctg ggaggtaacc aattagaggt acgctttcct
    53641 gagcatccac atcctctgtt tgtgatagca gagagcggct tcggcaccgg attaaacttc
    53701 ctgacgctat ggaaggcatt tgatcagttt cgcgaagcgc atccgcaagc gcaattacaa
    53761 cgcttacatt tcattagttt tgagaaattt cccctcaccc gtgcggattt agccttagcg
    53821 catcaacact ggccggaact ggctccgtgg gcagaacaac ttcaggcgca gtggccaatg
    53881 cccttgcccg gttgccatcg tttattgctc gatgaaagcc gcgtgacgct ggatttatgg
    53941 tttggcgata ttaacgaact gaccagccaa ctggacgatt cgctaaatca aaaagtagat
    54001 gcctggtttc tggacggctt tgcgccagct aaaaacccgg atatgtggac gcaaaatctg
    54061 tttaacgcca tggcaaggtt ggcgcgtcca ggcggcacgc tggcgacatt tacgtctgcc
    54121 ggttttgtcc gccgcggttt gcaggaagcc ggattcacga tgcaaaaacg taagggcttt
    54181 gggcgcaaac gggaaatgct ttgtggggtg atggaacaga cattaccact cccctgttcc
    54241 acgccgtggt ttaatcgtac gggcagcagc aaacgggaag tagcgattat cggcggtggt
    54301 attgccagcg cgttgttgtc gctggcgtta ttacggcgcg gctggcaggt agcgctttat
    54361 tgcgcggatg aagcccccgc gctgggtgct tccggcaatc gccagggggc gctgtatccg
    54421 ttattaagca aacacgatga ggcgctaaac cgctttttct ctaatgcgtt tacttttgct
    54481 cgtcggtttt acgaccaatt acccgttaaa tttgatcatg actggtgcgg cgtcacgcag
    54541 ttaggctggg atgagaaaag ccagcataaa atcgcacaga tgttgtcaat ggatttaccc
    54601 gcagaactgg ctgtagccgt tgaggcaaat gcggttgaac aaattacggg cgttgcgaca
    54661 aattgcagcg gcattactta tccgcaaggt ggttggctgt gcccagcaga actgacccgt
    54721 aatgtgctgg aactggcgca acagcagggt ttgcagattt attatcaata tcagttacag
    54781 aatttatccc gtaaggatga ctgttggttg ttgaattttg caggagatca gcaagcaaca
    54841 cacagcgtag tggtactggc gaacgggcat caaatcagcc gtttcagcca aacgtcgact
    54901 ctcccggtgt attcggttgc cgggcaggtc agccatattc cgacaacgcc ggaactggca
    54961 gagctgaagc aggtgctgtg ctatgacggt tatctcacgc cacaaaatcc ggcgaatcaa
    55021 catcattgta ttggtgccag ttatcatcgc ggcagcgaag atacggcgta cagtgaggac
    55081 gatcagcagc agaatcgcca gcggttgatt gattgtttcc cgcaggcaca gtgggcaaaa
    55141 gaggttgatg tcagtgataa agaggcgcgc tgcggtgtgc gttgtgccac ccgcgatcat
    55201 ctgccaatgg taggcaatgt tcccgattat gaggcaacac tcgtggaata tgcgtcgttg
    55261 gcggagcaaa aagatgaggc ggtaagcgcg ccggtttttg acgatctctt tatgtttgcg
    55321 gctttaggtt ctcgcggttt gtgttctgcc ccgctgtgtg ccgagattct ggcggcgcag
    55381 atgagcgacg aaccgattcc gatggatgcc agtacgctgg cggcgttaaa cccgaatcgg
    55441 ttatgggtgc ggaaattgtt gaagggtaaa gcggttaagg cggggtaatc tgctctcgca
    55501 gtgtttgccg gatgcggcgt gaacgcctta tccaacctac gtgtgacgtg taggcctgat
    55561 aagacgcgat aagcgtcgca tcaggcatgg tgctccagac gccgcaactt acagctgtga
    55621 cgcctgctga aacaggtttt cccacatatc ggtcaccagc gcctggtcac gcggcgacaa
    55681 ttcaccggca ccaatggctt tttccagact ctggctaacg gtcgtatgca ccgcctgagc
    55741 ggagtggtca tcaccacttt ccagttctgc gatggctaat gtcaggtggc cacgcaaata
    55801 cccactggca aacaactcat catcactggc atggtcaacc atgccgtcga ttaatgccag
    55861 aatgcgtgat tcaaactccg cgatcatctt ctttcctcat tgtaaaacgt ggcgctggct
    55921 tcagttgagc gcttccggcc acgggaactg ttccgccgta agttccggcg tgtgataata
    55981 attttgtagt gctttaatga agcgtgccgg gcgttccgga atgccatttg tcagataatc
    56041 catcacctgc gcatgtaccc gccgttgaaa caccacacga tccggttcga aatccccttc
    56101 cagattgtcg caactgacgt taaacggata tccagccgcc acgcagaata accagtcgaa
    56161 cgcctgcggc ttcacttcaa catcttcaaa ctggctttgc gtctgggcat cgcgtccgtc
    56221 cgggcaatac cagtagccga aatcaaccag ctcacggcgc gcttttccgg caatacacca
    56281 gtgcgaaatc tcatgaattg cgctggcata aaagccatga gcaaagacga ttcggttata
    56341 cggtacttcc gcatcagcag gaagatagat cggttcgtcg tcgcctttaa tcagacgggt
    56401 attaaattca tcggcaaagc agctattaaa aatttcaatc aactgctcgt agtggtgtgt
    56461 actgttcatt agttcatccc caaccagtgg aggatctcct gtccgtggct atcgtaaagt
    56521 aatttggcac tcatcaccgc cgagacgata acaatcatcg ggcggatcag cttttgtcct
    56581 ttgctcaaca ccaaccgtga acccatgcgc gcgccgagga actgccccac cagcatgaca
    56641 aagcccgtcg cccaaatcac tttgccgccg agaataaaca gcagcagacc gccaatgttt
    56701 gacgttgcgt tgagtaattt ggcgtgagcc gtggctttgg cgaggttgaa tccgcacagc
    56761 ataacgaagg ccagcgcgta aaacgatccg gcagccgggc caaagaatcc gtcataaaaa
    56821 ccgacgcagc caccggcaat taacgcgaag ggtaaaccgt acatccggcg ctggcgatct
    56881 tcttcaccca gctttggcat cagcaagaaa tagaggccga tacaaatcac cagaatgggc
    56941 aaaatctgcc gtaagacatc agcctgaacg tactgtacca acagtgcgcc gctcattgag
    57001 ccgacaaagg tcatggcaat attgagtttc tgatcgctta aactaaccac tttgcggcga
    57061 ataaagtaga tagtggcgga aatagagccg ccgcaggctt gcagtttatt ggttgccagc
    57121 gcgttagcgg gagacattcc cgcagccatc aatgccggaa tggtgagtaa cccaccgcca
    57181 ccggcaatcg agtcgataaa tcccgccagc atggcgacaa aaaagagaac tcccagcaac
    57241 agcggagaaa ccataaacag gctattaaac gtttccatta gatcacgtgc tcatccagta
    57301 gcgcctggca ggaaggcggc aacggaggcg gtgtcttctt ctcaggcttt gttgttcccg
    57361 gttttggagg ttcaaaccag ctttgcagtt ctgccccgca accatcgcct gatggcggta
    57421 aaggttgatc ttcacactcc agactatcgg cagggcaacg taatcgtaca tgcatatgcg
    57481 cgcgatgctg gaaccagggt cgcactttgc gcaaccagtc gcgatcggtg cccgcatcaa
    57541 ggcaaagttg ttgtttaatc gccggattaa caaaaatgcg cgtgacgtct ttgtcctggg
    57601 cggcgagttt gatcaagctg aaaatttctg gcttccacag cgtggagaca acgtgtttac
    57661 cgtcgcggga aactaagtcc agtgcttgcg ggcgcaagag ctgcgcggag gtccagcgag
    57721 ttttcggcag ttgcagaaag atatcgacat ccagtccggt ctggtggctg gcatgaccgc
    57781 cgttgaaacg cccaccagcg ggcatcccca tatcgccaat cagcaccgta cccatgccca
    57841 gattgctcac ctggctactc agacgctgga taaacatcac cagatccggg tgaccgaaat
    57901 agcgacgctg atcggtacgc atgacctgat aatgttcgga ctgtatcggc aacgtgtcag
    57961 cgccgacaat acagccatta gaaaaactgc ctatcgattg tgcgctaccc ggcacaggtt
    58021 gggttatttt ttgccacggc gtcgctgcca ggctagcact actggcaagc agagccagca
    58081 gcgcaatcgc ggttttgttc attttttacc agcgtggaat atcagtcttc acatcggcat
    58141 tttgcgcccg ctgccgtaac aggtgatcca ttaaaacgat cgccagcatc gcttcagcga
    58201 tcggcactgc acggatcccg acacagggat cgtgacggcc tttggtgatc atctcaactt
    58261 cttcgccaaa gcggttaata gtgcgccccg gcacggtaat gctggaggtc ggtttcagcg
    58321 ccatatgagc aatgatttgc tgcccgctgc tgataccgcc gaaaatgccg cccgcatggt
    58381 tgctctggaa accgtctttg gtgatttcgt cgcggttctg gctgccgcgc agcgccacta
    58441 cgtcaaaacc gtcgccaatt tccacgcctt tcaccgcgtt gatgctcatc agcgcatggg
    58501 cgatgtcggc atccaggcgg tcaaagaccg gctcgccaag tccggcgggg acaccactgg
    58561 caacaacggt gactttcgcg ccgatggagt cgccctcttt tttcagcgcg cgcatcagtt
    58621 catctaacgc gtcgattttg tcggggtccg ggcagaagaa cggattttgt tcaaccagcg
    58681 accagtcttt gatttccagc ggaatgtcgc ccatctgggt caggcagccg cggatttcaa
    58741 taccaaattt ctcggcgaga tattttttgg caatcgcccc tgccgccacg cgcatggcgg
    58801 tttcgcgggc ggaagaacgt ccaccgccgc gataatcgcg cagaccgtat ttttgttcgt
    58861 aggtgtaatc agcatggcct ggacggaaga cgtctttaat tgcgctgtaa tcctgagaac
    58921 gctggtcggt attttcgatc aacaagccaa tgctggtgcc agtagtaacg ccttcaaaaa
    58981 caccggagag tattttgacc tgatccggct cgcggcgctg ggtggtatag cgcgatgtcc
    59041 cagggcgacg acgatcaagg tcatgttgca ggtccgcttc cgtcagcgga atgcctggcg
    59101 gaacaccatc gacgatgcag ccgagcgcca gcccgtgcga ttcgccgaag gtggttacgc
    59161 gaaagagttg tccaattgtg tttccagcca tcacggctcc gttattgttg tgtttgcgtg
    59221 tttacttaat ctttataaat cgcgaaatgt tctcgtgcgg caataagctg ctctttagtg
    59281 agcataaaca caccatcgcc gccgttatca aactccagcc aggtgaacgg aacatcggga
    59341 tattgttcca taagatgtac catgctgttg cccacttcac aaatcaacac gccatcatca
    59401 gcaaggtaat ctgccgcgtt accgagaatg cgacgcgtca gtttcaggcc gtcggtgcca
    59461 gatgccaggc ccagttccgg ctcgtgacgg tattcgtttg gcaggtcgga catatcttcc
    59521 gcgtcgacat acggcgggtt agtgacaatc agatcgtatt gcactttcgg caaatcacgg
    59581 aacagatcgg aacgaatcgg aatgacgttg tggatcagac cgtgttcttc gatgttctgt
    59641 tcggcaaccg ccagcgcgtc gggagagata tctaccgcgt cgacttccgc ttccgggaag
    59701 gcataagcac aggcaatggc gatgcagccg ctaccggtac acatatctaa aatatgctgc
    59761 ggttgcttgc tgatgagtcc ggcaaattta ttgttgatca gttcaccaat cggcgagcgc
    59821 ggcaccagca cgcgttcatc gacgtaaaat tcatgaccgc agaaccaggc tttgttggtc
    59881 aggtaagcca ccggaatgcg ctcgttgacg cggcggatca cgcgttcaac aatacggtgt
    59941 ttttcgctgg aggtcagacg cgcggtgcgc atatcttccg ggatatccag cggcaggtag
    60001 agcgatggta acaccagctg tacggcttca tcccacgggt tatcggtgcc gtgaccgtac
    60061 cagatatttg ccgcgctgaa gcggcttacc gaccagcgca atatgtcctg aatggtttgc
    60121 agctcattta ctgcttcatc aacgaaaatt ttatccacgt attcctccag ggcatgatcg
    60181 caataatttc ggcggctagt ttgccatgaa gatgacgata aatcagcatt cacgcgcggt
    60241 gagtgaggaa aaatacgttt aaaacgatcg attgtgctac gagtcgggta aactgtagga
    60301 aaattagaaa tagagacaga taaatgaaaa agaaaacaac acttagcgag gaggaccagg
    60361 ctctgtttcg ccagttgatg gcggggactc gcaagattaa gcaggacacg attgtccacc
    60421 gaccgcagcg taaaaaaatc agcgaagtgc cggtgaaacg cttgatccag gagcaggctg
    60481 atgccagcca ttatttctcc gatgagtttc agccgttatt aaataccgaa ggtccggtga
    60541 aatatgttcg cccggatgtc agccattttg aggcgaagaa actgcgccgc ggcgattatt
    60601 caccggaatt gtttttggat ttacacggtc tgacgcagct gcaggccaag caggaactgg
    60661 gcgcgttgat tgccgcctgt cgccgcgaac atgtgttttg tgcctgcgtg atgcatgggc
    60721 acgggaagca tattttgaag caacaaacac cgctgtggct ggcacaacat ccgcatgtga
    60781 tggcatttca ccaggcaccg aaagagtacg gcggtgatgc ggctttgttg gtgttgattg
    60841 aagtggaaga gtggttgccg ccggagttgc cctgaatgct ggcagcaaat gccggagcag
    60901 cgtaaacgcc ttatccggcc tacaaatatg tgataattca ataaattgca attcacttcg
    60961 taggcctgat aagacgcatc agagcaatac tacgaacttc agttcattca atacatactg
    61021 aaaacgacac gtcgaatcaa tcggttgccc actactggta ttcacattca ccacaatatc
    61081 gtgtagtact ttatccgtta ccggatgacg agttggcgta accgttaccg ttgatttatc
    61141 acaaccgtaa gaggtaaagg ttaacaattc tgataccgcg cggcagtatg ctcctctgcc
    61201 atcattatca gaaaaattac tgccatcaca gagtctgccg ccttccatca ggtaggtcga
    61261 aagcgtggtg gtcactgttg accccgtgga ctgaattcgt aacgccttat tatggctgac
    61321 ataattttca ctccaggaga tatcgtatga agcgaccggt atcgttgggc atattgtgtt
    61381 atgcactgta ggggccgtat agcttccaaa tccgggtaaa tctgtatagg aataaaagga
    61441 tgcgatccaa gggcaaccat aatcatcaga tgcaacggta attttaaacg tcatatatgc
    61501 acaacgaaaa attttcgtat ctatgccagg gcaatttgag ttattaattg tatcaaagtc
    61561 agtcagtacc aaagttggag ctgaacccga cccagaccag gaaataaccc gcaacgtatc
    61621 gcctttatac gcaaaagaat catatttccc atatgcaacc tcctttggcg ttatcaccgt
    61681 aacattgaaa acactgctcg ctacagcacc ggttaccttg tccatggcat aataaactct
    61741 cacactgtca aaactcgcac tcataagttt atctccagca taactcttag atgcgccgac
    61801 aacactcaga acgagcagca ttaggctcgc gaaaattatg gtccattttt tcatttcaga
    61861 aatactcaaa ggttgccgtc actgtggcac taaagtctcc ggacgttaca tctcgcccgc
    61921 tctttgcctg taaccaggta ttaaaattta ggctgttatt tccctgattc agcgggagcg
    61981 tgacgcctgt tgtattgttg attggcaccg atgtgcctgc cgcagtttca ataccaatcc
    62041 cagcgccgga tgctgatgac gaggagtcaa aggccagaaa gccgggtaga gcactatctt
    62101 ctgttcctgt caacgttact ttgacattaa acaatgtaga actatgacaa tctttcaatt
    62161 gaaacaccaa tggcactcgc tctgactgcc caaggttcat taaatcactg gcggcaatgg
    62221 caggaaaatg tacttctgcc aactggctac cattaatcac tggcgtacac gatttactga
    62281 ttaaattgcc ggtaaagtgc aaattatctc ctgctattgt ggttccactg cccagcaaca
    62341 tcaggctact aataaaaaac aacgtacgct ttttcttcat tggtaatcta cctgtaacgt
    62401 ggcccaggcc tcaaagtccg cctctggcag aatgacacca ctctttttta ctggcacagc
    62461 cgttaacacc ggtaaatcgg tttcattcac cacaagtggc gtattgatcg taaaactctg
    62521 ccctgcctgt ttgagctgaa tgcctaaacc agtgatattg ctgcttactg cagcattatc
    62581 aaaatcgctt ggtgtgccac tccagctcaa ggtcatttcc attaatgggt cgcgaaccgc
    62641 cgtatcgcag gtaatggaat acggcacggt ttgctcataa ttgctgccat taatgtcatc
    62701 aataagcaca tcctgaaaag ttatttcaat ggtttgagcg ttgctaatcg tgcagttagg
    62761 cggctgcact aaataaccgt gaaaagagac gtttgagagt gccatcgagc aaaagcccca
    62821 cagcaaaata agcgagatcc gtttcattgg taatccacca caattgtggc actggcgtta
    62881 aattcagctg ccgctaacgt tgtaccactc tgtttcaccg gcacagcttc cagaacggga
    62941 actcccgagg aaaaattaaa tggcaaccat gagccagagg ttaagtcgag tattgtgtga
    63001 tcactacttt tctgaacccg aataccaaac ccaggaatac ccgtgctgat aactgtttca
    63061 ccgttaatca cgaccgttgt cgcctgcatc tgtagacgca ggtcatccat tgccagattg
    63121 ttacaaacaa gcgagtagtc aatgggacgt ttataatcaa ccctattgat cttattaatg
    63181 aatacattac caaactcaac tgccgcacca tttaccgtac aaggaggagg ggcatcgaca
    63241 agcacccgta aattcatact tactgactta ctcgctgcaa ctgcaaagcc gctaccaaca
    63301 caacatatca gtaacccagt taccgcccag acagaacaag atcctttcat actggctctc
    63361 ccggcctcag cctttgcggc tctgctcttc atcgacctag caactgttac cgttgcaagt
    63421 gaaaaagagc ggcatccgag cgccatagtc gttgacatag gtcagtaccg gcacgctacc
    63481 catcttcaca ttcagcggtg cagaggtttt cggcgacatc actattggcg agaaaccggc
    63541 tgccggattg ccatttttct gtgtgctggc attactgaaa atgacgtaat acgacgtcgg
    63601 gttattgacg gtaaattcct gaccgttgcg ggttaaagta atcttatatt gctaggggtt
    63661 tttcatgctg acgttctcca gcgccttcgg tcgccagaac aacttaatac gcgtttgtag
    63721 tgctatttgc agcgtgttgg ctttgtttga ttttggcggg atctcacgca cgttgaagta
    63781 gaacaggctt tcacgatcgg caggcaattt gttgatatcc ggcattccct gcacttttac
    63841 ctgcccgttc atcattgaat cgatgcgttg caccggtggc agaacagtta acggtgaact
    63901 gatcttgtta cccttttcat cttcgatcca gctttgggcc agataaggca gtttcggatc
    63961 gttattgcgc aacgtcacgc tgattgattt gtcgctttca ttaaatatca aacgggtacg
    64021 atctggcgtt acagaagcct gggcagacaa actggcagcg ctaagcagta acgctagcgt
    64081 cgtagcaccc aatttggcag agcaaagtaa atcagacatg gatcattcct taatcacgtt
    64141 attggttagc tgaaacagaa acagggggat tggtgggtgt cacctgttgc gtctgctcct
    64201 gaatgacagg ctttatgtca tcaggaacaa ccggcgcgac gttgccttta tgctggcatg
    64261 gcagtaacaa gccgttaaac agatcggcag gcagcgggtc cggcaggttg atatcgcaat
    64321 gcgcaacacc actccagaac acactcatgt gttcgccagg tttcactcca gccagataaa
    64381 cattgccgtc atcatcgaca aggcccactg tctgctcgtt atcatttttt acttctgcgc
    64441 caaacggagg atggctgccg tcttgtaaac gcagcaccgc catcgctttt tgaccactga
    64501 tgacggcaaa tttgcggtag ccaatcgccc cttcagttag cgtggcttgt accaccgact
    64561 gggttgcttc ggcgttttcc ggcagtttgt tgaggtcgat atacgcctga ttgcggtaat
    64621 agttattgac gtcagaaacg acggctttac caaacatatt ggtataaaca gcagcaccgt
    64681 taccttctac cggaacatcg gcaacgccat cggcatcaat caacaagcgt gtcccgccca
    64741 tattctgggt acggtgaagt gcgccaccgt gggcagtaag cgttgcgccg ccctgtaacg
    64801 acaagcccgc agaggtgtat tgcccttcat ggtagttcgc actgaggtca acctgcgcca
    64861 gcgaaccatc atggctgtaa tagccgtcaa cgctggtgtg tttgtcactg gtgccgacgt
    64921 tcaactgata gtgagtcgcg tcatcgacac ggctgaaata accgacctga ctgctgtccg
    64981 tcccactgcc atagtaccgt tatagctaac ggtactgttg tcgccccacg gcatactgag
    65041 cgaaatgtac atgcctttgt cggcctggtt gtcatactcg taacggtagc cagtcatcga
    65101 gatgctgaca ttacgaatac tgcccatatt gaaatagtgc gagagcatga tgttgtagtt
    65161 tgtctgctcc tcgcgatccc agtaggtatg gcgggtatag ttgagataaa ccgaaacacc
    65221 cgcatcgcgg aagttctggt tataagtggc ggtgtacatc tctttgtcgt tgcccgtgcg
    65281 taccattccg ctgtcactgg catccagata ctcgctcatg gtcataaagt tctcttccga
    65341 gaagcgatat ccagcaaaag taacgcggct gttgagctgg tcaaagtctt tggaataact
    65401 cacacggaag gagttaccgt ccagcgaacc tttgccataa gcggtgtctt tatccagttt
    65461 ggtgtgcgag tgagtaacat caaacgcgac cgcgccgaat gtagacaaat cgcgaccgac
    65521 accaagcgcc gcagactgat agttttcatc tcccagtgcg ccgccatata acgaccagcc
    65581 gttagcgatc ccccaggaag cttcagcacc agaaaaaaat tcaccctcga catggtgtcc
    65641 ccactcttgc ggacggccca tcatgatctt atagcgaacc tgacctggac gagtgaggta
    65701 tggcatcgag gcggtgctga tgtcatattc ctgcacctgg ccgttctgtt cttcaatgcg
    65761 aatatgcaac gtaccggaga cagaatcacc aagatcctga atacgaaacg gtccagccgg
    65821 aacctgcgtt tcgtaaatca cacgccccat ctggctgacg gtcacttttg ctgtggtgtg
    65881 tgccacgccg gaaatgtctg gcgcgtagcc gcgcagattg ggaggcaaca tttgatcgtc
    65941 agtactgaca ctgccaccaa catagttaaa accatcaaaa atatcggatc tgaggtaatc
    66001 ctcgcccagc gccagtttgg cttttaatga tggtaacgcc cgcctggcat agtagcgact
    66061 ccactcccat ttttttgagt ttcatcgccg ctgaattcat cgtcatcatt actgcgagta
    66121 tgttgatagt tagtctgcca gtcagcacgc atacgccacg gccctaggtt aaccccaacc
    66181 gtcccgttgc cgctgatctc gttactatca tcaccgccat tttcttcgtg ccgggtttgt
    66241 gcgttgatgc tgtagtccgc gacgatcccg gagatgccgt catcccaacg tgaaggcgga
    66301 tccctgtcgg gataagtata ttcgaggtaa gcctgtggca gtgaaatgac taatgcggac
    66361 tggcttaaat cagccttaat ttccatgcct tccagttgac cggatttcag gcatttagca
    66421 tcgtggctcc attgcagatt tttcgccacg tcttctttta aaccaaactg cgctaccagt
    66481 tccggtgtca gacaagcata gcttttgctc gcgtcatctt caccagcata ccagtaaata
    66541 tcgtactctt ccgccaatgg ctgtttattt agttgaacct gtaaattgta ttttccgggc
    66601 tcaacatatc cctggctgga aaaacgcttc atatcaattt ttgtgtcgcc ttttaattcc
    66661 agaaaacggg aatcaaactg aatgtcgtct tcagcccaga cactgctata actccctgac
    66721 attgccagcg caatgcacca cggaagaatc cgtaatcgaa aaagagaatg gtcaggcata
    66781 cacgtttcta tccgttttta ttcttgttcg gtcaaaatac atttaaatcc cccatagcag
    66841 tgtcgctatg ggggtatgcc ttacaggtaa gtaatctgga aggtggtatt tgcttcaaaa
    66901 ttacccagat ctggcgcatc agctgcgccg accagccagg cgttaaagtt cagtgtctgc
    66961 ttcgctttgc ctttgttggt agaagtgtct ttcactattt tctggtcaat acccatgccg
    67021 cttttgtaag aggtacccag agagtcaccg atcgccaggc taacattgtt aaatgccgca
    67081 ccggtatcgg tattgaaaat ggtgtaataa ttgccgctat ttgcagaaga aacggtacca
    67141 gtaaaggtag tggtcattgt ttcctgagta tcaaatacgc aatcttgcag acgaatctgg
    67201 aaacttttcg gcgtggtggt gccgttattt ttcagttttt cggcaccgat atcacccata
    67261 tcaacttcca gtttgtgatc gtccggaacg atagaacaca ctgagtcttc aatggtgccg
    67321 taaaaacgtg cagtaccgtt gttacccgca gcgattgttg ccgtagaggt gaacacgccc
    67381 atcaccattg ccgcagcaat agctgtttta acaaacttac tcattatttg tccttaaact
    67441 ataaataatt aaaaatataa acattgcaat acattgattc agtcaatagc caatgtttat
    67501 acccagccat tgaatacggc atgcgaccca aaaagaacag gtcgtagaaa attgtattca
    67561 ttggtgtggg gccgcatagt gatgtttcca cacccctgat ccaatttaat tttactaaag
    67621 agtaaatgta gatttaatta atatattgac agaagaaagg ttatcttaag gac
//
LOCUS       DAJTQQ010000008        66645 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000008 DAJTQQ010000000
VERSION     DAJTQQ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66645
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.008"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(33..2480)
                     /gene="glgP"
                     /locus_tag="OO309_000596"
     CDS             complement(33..2480)
                     /gene="glgP"
                     /locus_tag="OO309_000596"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR5845858.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2499..3932)
                     /gene="glgA"
                     /locus_tag="OO309_000597"
     CDS             complement(2499..3932)
                     /gene="glgA"
                     /locus_tag="OO309_000597"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR5845859.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3932..5227)
                     /gene="glgC"
                     /locus_tag="OO309_000598"
     CDS             complement(3932..5227)
                     /gene="glgC"
                     /locus_tag="OO309_000598"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR5845860.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5245..7218)
                     /gene="glgX"
                     /locus_tag="OO309_000599"
     CDS             complement(5245..7218)
                     /gene="glgX"
                     /locus_tag="OO309_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR5845861.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7215..9401)
                     /gene="glgB"
                     /locus_tag="OO309_000600"
     CDS             complement(7215..9401)
                     /gene="glgB"
                     /locus_tag="OO309_000600"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR5845862.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9674..10777)
                     /gene="asd"
                     /locus_tag="OO309_000601"
     CDS             complement(9674..10777)
                     /gene="asd"
                     /locus_tag="OO309_000601"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5845863.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10969..11562
                     /gene="yhgN"
                     /locus_tag="OO309_000602"
     CDS             10969..11562
                     /gene="yhgN"
                     /locus_tag="OO309_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR5845864.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11619..12959)
                     /gene="gntU"
                     /locus_tag="OO309_000603"
     CDS             complement(11619..12959)
                     /gene="gntU"
                     /locus_tag="OO309_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5845865.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12963..13490)
                     /gene="gntK"
                     /locus_tag="OO309_000604"
     CDS             complement(12963..13490)
                     /gene="gntK"
                     /locus_tag="OO309_000604"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR5845866.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13629..14624)
                     /gene="gntR"
                     /locus_tag="OO309_000605"
     CDS             complement(13629..14624)
                     /gene="gntR"
                     /locus_tag="OO309_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR5845867.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14848..15543)
                     /gene="yhhW"
                     /locus_tag="OO309_000606"
     CDS             complement(14848..15543)
                     /gene="yhhW"
                     /locus_tag="OO309_000606"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR5845868.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15666..16703)
                     /locus_tag="OO309_000607"
     CDS             complement(15666..16703)
                     /locus_tag="OO309_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5845869.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17036..17524
                     /gene="yhhY"
                     /locus_tag="OO309_000608"
     CDS             17036..17524
                     /gene="yhhY"
                     /locus_tag="OO309_000608"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5845870.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17583..19325)
                     /gene="ggt"
                     /locus_tag="OO309_000609"
     CDS             complement(17583..19325)
                     /gene="ggt"
                     /locus_tag="OO309_000609"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR5845871.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19445..19885
                     /locus_tag="OO309_000610"
     CDS             19445..19885
                     /locus_tag="OO309_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR5845872.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNSDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19872..20615)
                     /gene="ugpQ"
                     /locus_tag="OO309_000611"
     CDS             complement(19872..20615)
                     /gene="ugpQ"
                     /locus_tag="OO309_000611"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5845873.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20612..21602)
                     /locus_tag="OO309_000612"
                     /pseudo
     CDS             complement(20612..21602)
                     /locus_tag="OO309_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21604..22449)
                     /gene="ugpE"
                     /locus_tag="OO309_000613"
     CDS             complement(21604..22449)
                     /gene="ugpE"
                     /locus_tag="OO309_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR5845874.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22446..23333)
                     /gene="ugpA"
                     /locus_tag="OO309_000614"
     CDS             complement(22446..23333)
                     /gene="ugpA"
                     /locus_tag="OO309_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR5845875.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23431..24747)
                     /gene="ugpB"
                     /locus_tag="OO309_000615"
     CDS             complement(23431..24747)
                     /gene="ugpB"
                     /locus_tag="OO309_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR5845876.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25144..25857)
                     /gene="livF"
                     /locus_tag="OO309_000616"
     CDS             complement(25144..25857)
                     /gene="livF"
                     /locus_tag="OO309_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR5845877.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25859..26626)
                     /gene="livG"
                     /locus_tag="OO309_000617"
     CDS             complement(25859..26626)
                     /gene="livG"
                     /locus_tag="OO309_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR5845878.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26623..27900)
                     /gene="livM"
                     /locus_tag="OO309_000618"
     CDS             complement(26623..27900)
                     /gene="livM"
                     /locus_tag="OO309_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR5845879.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27897..28823)
                     /gene="livH"
                     /locus_tag="OO309_000619"
     CDS             complement(27897..28823)
                     /gene="livH"
                     /locus_tag="OO309_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR5845880.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28871..29980)
                     /gene="livK"
                     /locus_tag="OO309_000620"
     CDS             complement(28871..29980)
                     /gene="livK"
                     /locus_tag="OO309_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR5845881.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30404..30787
                     /gene="panM"
                     /locus_tag="OO309_000621"
     CDS             30404..30787
                     /gene="panM"
                     /locus_tag="OO309_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR5845882.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30986..32089)
                     /gene="livJ"
                     /locus_tag="OO309_000622"
     CDS             complement(30986..32089)
                     /gene="livJ"
                     /locus_tag="OO309_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR5845883.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32360..33214)
                     /gene="rpoH"
                     /locus_tag="OO309_000623"
     CDS             complement(32360..33214)
                     /gene="rpoH"
                     /locus_tag="OO309_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR5845884.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33459..34517)
                     /gene="ftsX"
                     /locus_tag="OO309_000624"
     CDS             complement(33459..34517)
                     /gene="ftsX"
                     /locus_tag="OO309_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR5845885.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34510..35178)
                     /gene="ftsE"
                     /locus_tag="OO309_000625"
     CDS             complement(34510..35178)
                     /gene="ftsE"
                     /locus_tag="OO309_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR5845886.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35181..36677)
                     /gene="ftsY"
                     /locus_tag="OO309_000626"
     CDS             complement(35181..36677)
                     /gene="ftsY"
                     /locus_tag="OO309_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR5845887.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSSEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36827..37423
                     /gene="rsmD"
                     /locus_tag="OO309_000627"
     CDS             36827..37423
                     /gene="rsmD"
                     /locus_tag="OO309_000627"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR5845888.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37413..37682
                     /locus_tag="OO309_000628"
     CDS             37413..37682
                     /locus_tag="OO309_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR5845889.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37685..38044)
                     /locus_tag="OO309_000629"
     CDS             complement(37685..38044)
                     /locus_tag="OO309_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR5845890.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38185..38811
                     /locus_tag="OO309_000630"
     CDS             38185..38811
                     /locus_tag="OO309_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR5845891.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38885..41083
                     /gene="zntA"
                     /locus_tag="OO309_000631"
     CDS             38885..41083
                     /gene="zntA"
                     /locus_tag="OO309_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR5845892.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41185..41430)
                     /gene="tusA"
                     /locus_tag="OO309_000632"
     CDS             complement(41185..41430)
                     /gene="tusA"
                     /locus_tag="OO309_000632"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR5845893.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41651..42316
                     /locus_tag="OO309_000633"
     CDS             41651..42316
                     /locus_tag="OO309_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR5845894.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42389..42946
                     /gene="dcrB"
                     /locus_tag="OO309_000634"
     CDS             42389..42946
                     /gene="dcrB"
                     /locus_tag="OO309_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR5845895.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42950..44167)
                     /locus_tag="OO309_000635"
     CDS             complement(42950..44167)
                     /locus_tag="OO309_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5845896.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44301..45350
                     /locus_tag="OO309_000636"
     CDS             44301..45350
                     /locus_tag="OO309_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5845897.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45405..45992
                     /gene="acpT"
                     /locus_tag="OO309_000637"
     CDS             45405..45992
                     /gene="acpT"
                     /locus_tag="OO309_000637"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR5845898.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46103..47677
                     /gene="nikA"
                     /locus_tag="OO309_000638"
     CDS             46103..47677
                     /gene="nikA"
                     /locus_tag="OO309_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR5845899.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47677..48621
                     /gene="nikB"
                     /locus_tag="OO309_000639"
     CDS             47677..48621
                     /gene="nikB"
                     /locus_tag="OO309_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR5845900.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48618..49451
                     /gene="nikC"
                     /locus_tag="OO309_000640"
     CDS             48618..49451
                     /gene="nikC"
                     /locus_tag="OO309_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR5845901.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49451..50215
                     /gene="nikD"
                     /locus_tag="OO309_000641"
     CDS             49451..50215
                     /gene="nikD"
                     /locus_tag="OO309_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR5845902.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50212..51018
                     /gene="nikE"
                     /locus_tag="OO309_000642"
     CDS             50212..51018
                     /gene="nikE"
                     /locus_tag="OO309_000642"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR5845903.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51024..51425
                     /gene="nikR"
                     /locus_tag="OO309_000643"
     CDS             51024..51425
                     /gene="nikR"
                     /locus_tag="OO309_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR5845904.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51545..51904)
                     /locus_tag="OO309_000644"
     CDS             complement(51545..51904)
                     /locus_tag="OO309_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR5845905.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51901..52176)
                     /locus_tag="OO309_000645"
     CDS             complement(51901..52176)
                     /locus_tag="OO309_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR5845906.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52235..53359)
                     /locus_tag="OO309_000646"
     CDS             complement(52235..53359)
                     /locus_tag="OO309_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5845907.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53359..56094)
                     /gene="rbbA"
                     /locus_tag="OO309_000647"
     CDS             complement(53359..56094)
                     /gene="rbbA"
                     /locus_tag="OO309_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR5845908.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIQARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56091..57158)
                     /locus_tag="OO309_000648"
     CDS             complement(56091..57158)
                     /locus_tag="OO309_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5845909.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57524..59146)
                     /locus_tag="OO309_000649"
     CDS             complement(57524..59146)
                     /locus_tag="OO309_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5845910.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59408..60997)
                     /locus_tag="OO309_000650"
     CDS             complement(59408..60997)
                     /locus_tag="OO309_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5845911.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61382..62413
                     /locus_tag="OO309_000651"
                     /pseudo
     CDS             61382..62413
                     /locus_tag="OO309_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62500..62976)
                     /locus_tag="OO309_000652"
     CDS             complement(62500..62976)
                     /locus_tag="OO309_000652"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5845912.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62969..63733)
                     /locus_tag="OO309_000653"
     CDS             complement(62969..63733)
                     /locus_tag="OO309_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR5845913.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGIAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63979..64740
                     /locus_tag="OO309_000654"
                     /pseudo
     CDS             63979..64740
                     /locus_tag="OO309_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(64847..65347)
                     /locus_tag="OO309_000655"
     CDS             complement(64847..65347)
                     /locus_tag="OO309_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5845914.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(65498..>66645)
                     /locus_tag="OO309_000656"
     CDS             complement(65498..>66645)
                     /locus_tag="OO309_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5845915.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAIQPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT        16192 a        17890 c        16345 g        16218 t
ORIGIN      
        1 accctttggc cccgttctat ttattagtga aattacaatc tcaccggatc gatatgccag
       61 atatgatcag cgtactcttt gatagtacgg tcagaagaga agtagcccat attggcaatg
      121 ttcagcatcg cttttgcggt ccactcttcc tgaagctcgt agagttcgtc gactttatcc
      181 tgacaatcga catagctgcg ataatccgcc agtacctggt agtgatcgcc gaagttgatc
      241 agcgaatcaa ccagatcgcg atagcgaccc ggatcttccg gactgaatac accgctgccg
      301 atttgcgtca gcacctgatg cagctcctca tctttctcgt agtattcacg cggtttgtag
      361 ccctgacgac gcagttcttc cacttcttcc gctgtgttac caaaaataaa gatattgtca
      421 gcaccgacat gatccagcat ctcgacattc gcaccgtcca acgtaccgat agtcagcgca
      481 ccgttaagcg caaacttcat gttactggtg ccggaagctt ccgtccctgc cagcgaaatc
      541 tgttcagaca gatctgccgc cggaatgatc aactgcgcca ggctaacgct gtagttcggg
      601 atgaacacga ctttcagctt atcgccaatc tgcggatcgt tgttgatcac tttcgctacg
      661 tcattgatca aatgaataat gtgcttcgcc atgtaatagg ccgaagccgc cttaccgcca
      721 aaaatattca cgcgcggtac ccacttcgca tccgggtcgg ccttgatgcg gttatagcgg
      781 gtaatcacat gcaacacatt catcaattga cgtttgtatt cgtgaatacg tttgatttgt
      841 acatcgaaca acgcctttgg attcaccacc acattcagct gctgggcgat atactctgcc
      901 agacgctttt tgttctccag cttcgcctga tgcacagcgt gattaaccat tgggaaatca
      961 cagtgttgtt gcagctcatt aagcaggcta aggtcggtgc gccagttacg gcccaggtgt
     1021 tcgtccagca cggctgaaag cgatgggttc gctaccgcca gccagcgacg cggcgtcaca
     1081 ccgttggtga cgttggtgaa acgacccggg aagattttcg caaagtcggc aaacaacgat
     1141 tgcaccatca gattagagtg cagttccgat acaccgttaa ctttgtggct cacaacaacc
     1201 gccagccagg ccatacgcac acgacgaccg ttggattcat caatgatcga cgcccgtccc
     1261 agcagatcgg tatcgttcgg atactgttcc tgcaaggttt tcaggaaata gtcgttgatt
     1321 tcaaagatga tctgcaggtg acgcggcaga attttaccca gcatatcaac cggccaggtt
     1381 tccagcgcct cgctcatcag cgtgtggtta gtgtaggaga agacctgaca acacacctca
     1441 aacgcgtcgt cccagctaaa ttggtgctca tcgatcagca gacgcatcat ctcaggaatc
     1501 gacagtaccg gatgggtatc attgagatga atcgcgattt tatccgccag gttatcgtag
     1561 gttttatgca actgataatg acggcttaaa atgtcctgaa tggtcgagga aaccaagaag
     1621 tattcctgac gcaggcgcag ctcacgcccg gagtaggtgg agtcatccgg atacagtacg
     1681 cgagatacgt tctcggagtg gtttttatct tccactgccg cgaagtagtc accctggttg
     1741 aatttaccga ggttaatttc gctactggct tgcgcactcc acaaacgcag cgtgttggtc
     1801 gcgtcggtgt cgtaaccagg gattatctga tcgtaagcga ctcccagaat ctcttcggtt
     1861 tcaatccagc gcgttttttt accttcctgc tgaatgcgac caccaaaacg gactttatag
     1921 cgcgtgttgt ggcgtttgaa ttcccacggg ttaccgtatt ccagccagta gtctggcgac
     1981 tctttctggc taccgttaac gatgttctgc ttgaacatac cgtagtcata gcggatgccg
     2041 taaccgcgcc ccggcaaccc taacgtcgcc agagaatcaa ggaagcaagc cgccagacgt
     2101 cccaggccac cgttaccgag gcctgggtca ttttcttcat caatcagctc ttcgagattt
     2161 aaccccatcg cttccagtgc gccctgtaca tcttcgtaaa ttcctagcga caacatggcg
     2221 ttggagagcg tacggccaat caaaaactcc atcgacaggt agtaaacctg acgagtttct
     2281 tgcgacaact gggcacggtt tgaacgtaac cagcgctcca cgagacgatc gcgcacagca
     2341 aataacgttg cgttcagcca ttcatgttta ttggcgacga ccgggtcctt tccaatcgta
     2401 aacatcagct tgtaagcgat agagtgctta agagcttcta cgctaagcgt gggcgatgaa
     2461 tatgtaaacg gagcattcat ataggcgttt cctgaaaact atttcaagcg atagtaaagc
     2521 tcacggtacg acttcgccgc gacctgccag ctaaaatcca ttgccatagc ctgacgttgc
     2581 acaaaccgcc acagtgaagg acgggaccac agtacaaaag cacgccgaat agcccgtaac
     2641 agcgaccagg cattactatc ttcaaagaca aacccactgg cgacgccatc tgcaaggttc
     2701 tcaagagaac agtcagaaac cgtatcagca agcccaccgg tgcgccgcac taacggcagc
     2761 gtaccgtact tcaatccata aagttgcgtt aagccgcacg gttcaaaacg gctgggcacc
     2821 agaatgacgt ccgcgccgcc cataatgcga tgcgaaaatg cttcgtgata gccaatctga
     2881 acgcccacct gaccggggta ttccgctgcc gccgcaagga aaccttcctg cagcaccgga
     2941 tcgcccgcgc cgagtagcgc cagctgcccg ccctgctcca gaagccccgg taaggcttcc
     3001 agcaccaggt cgagaccttt ctggctggtc agacggctca ccactgcaaa aagcggcact
     3061 ttatcgtcaa ccttaagccc cattgcgatt tgtaactggc tcttattttc cgctttatct
     3121 tccaacgtat cgcgggtgta acgcgaggcc aacagtaagt ccgtctctgg actccagatt
     3181 ttctcgtcca cgccgttcag tacgcctgaa agacgccctt cacggtgacg ctgttgcaac
     3241 agaccttcca taccgtaggc aaactgcggt tcggtgatct cgcgagcgta ggttggactg
     3301 accgccgtaa tgtgatcggc atagtacaga ccggccttca ggaaagagat ttgtccgttg
     3361 aattccagcc catgaatatt aaagaatgac catggcaatt ggatgtcatt catgtgatgt
     3421 gcataaaaca tgccttgata ggccaggttg tgcacagtaa acaccgactt cgcaggacgc
     3481 ccgcgcgccg ccagatacgc aggcgcaaga cctgcatgcc agtcgtgcac atgcaccaca
     3541 tcaggacgcc agaatgggtc aagcccgctg gccatttctg ccccaaccca ccccagcagc
     3601 gcaaaacgca atacgttgtc ggtataggca aataagttgg tatcgtgata cgggcttccc
     3661 ggacgatcat agagatgcgg cgcgtcaatc aggtatatgc caaccccgtt gtaatgaccg
     3721 aacaacagcg tgatatgccc ggcgaaagta tcacgacggg atactacctg cgcatcggtc
     3781 acgccacggc gaatatcggg aaatgcaggc aacagtacgc gagcgtcaac gccgtctgcg
     3841 atttgtgctg cgggtaatgc cccaataaca tcagccagac cgccggtttt aagcagcggg
     3901 aacatctctg aacatacatg taaaacctgc attatcgctc ctgtttatgc cctaacttcc
     3961 gtagcatttc gcgcgttacc agcacgatgc cttcttctga acgatagaaa cgacgtgcat
     4021 cttcctctgc gttttcacca atcaccatgc cttccgggat aacacaagca cgatcgatga
     4081 cgcagcggcg cagacggcac gagcgaccta cccatacttc cggtaacaat acggcggaat
     4141 caatgttgca gaatgaattc acgcgaacgc gcgagaacag aacggactgc accaccaccg
     4201 aaccggagat cacacaaccg ccggatacca gtgagttaag ggtcatcccg tggctaccgg
     4261 agcgatcctg cacgaatttc gctggcggta atgattcatt gtaggtgcga attggccaat
     4321 tgcgatcgta catatccagt tccggcacca cagaggccag atcgagattc gctttccagt
     4381 aagcttccag cgtacccaca tcgcgccagt acggctcggc atccgggtcg gattgtacgc
     4441 aagagagcgg gaacgggtgc gcataggcca gaccggcttc ggtgatcttg ggaatcaaat
     4501 ctttgccaaa gtcgtggctg gagttctcat cacgatcgtc ttcttccagc agttcataca
     4561 gatagtcggc gtcaaagacg tagataccca tactcgccag agatttgctc ggatcgttcg
     4621 gcattgacgg tgggttagca ggtttttcaa cgaattcgat aattttatcg ttctcatcaa
     4681 ccgccataac gccaaatgcg gaggcttctt caatcggtac tggcatacaa gcaacggtgc
     4741 aacgtgcgcc tttttcgacg tgatcgataa gcatacgcga gtagtcttgc ttgtagatat
     4801 ggtcgcccgc caggatcacc acgtattccg ctttataacg gcggataatg tcgaggtttt
     4861 gggtgaccgc atctgcggtg ccgcgatacc agttttcccc tttcattctc tgctgtgctg
     4921 gcagcagatc gacaaactcg ttcatttctt cattgaagaa tgaccagccg cgctgaatgt
     4981 gctgcaccag agtgtgggac tggtactggg tgatcacgcc catacgacgg atcccagagt
     5041 tgatgcagtt agacagcgca aagtcaataa tgcggaactt accgccgaag tgtacggccg
     5101 gttttgctcg cttattggtt aaatccttca ggcgggtacc acgtcctccc gccagtatca
     5161 gggcaacaga tttcaatggc agctggcgcg ccaacattaa gtgatcgttc ttctctaaac
     5221 taaccatgac taactccttt tttatcatct ctggaacaca cacaatccgt gtgcaggtcc
     5281 ctgccagaca accgtaatca ctgggttatc ctctccagcg aatgggggaa tggcgtgcca
     5341 ctccccagca ggtaaaacaa tctctgttac ctcaagcgtg gcgttaattg cgatcaaaaa
     5401 gcgatccgag agcagaattt gcagctgttt cggcccgttt tgccactcat ccgtgcttaa
     5461 aggttgagca tatcgattta gccaacggac attgccgtca ccttcttccc accagcgatt
     5521 ctccaccaaa gcgggaatgc gcttgcgcag atggattaac gcggcggtaa atgcggttaa
     5581 accactgctt gcctgcgacc agtccaacca gtttaattgg ttatcctgac agtaggcatt
     5641 gttattgcca tgctggctgt gaccatgttc gtcaccggcc agtaacatcg gcgtgccctg
     5701 ggagagcaac aacgttgtta acagggcatg aatgctgtcg cgccgccgtt caaccaggtc
     5761 aagagtaccg cctaaccctt ctttaccatg attgttactg taattgttgt tggtcccgtc
     5821 gcgattttct tctccgtttg cttcattgtg tttatggttg aagcaaacgc agtcgcgaag
     5881 cgtaaaaccg tcatgcgcgg tgacgagatt aatcgcggca ctcggcagac gaccattacg
     5941 tttaaaaaca tcgctggagg cagcaaaacg cccggcaaac gcccccagag gcaaatcata
     6001 atgtagccag aaacgacggg cagcatcgcg gaaatgatcg ttccactcgg caaacagcgg
     6061 cgggaaattt cccacctgat aaccactagg agcgatatcc cacggttcag caattaactt
     6121 cacctgcgag agcaccgggc agttctggat agcggtaaac aacggcgcat cctgacggaa
     6181 ctctggcgta cggcccatga ctgccgccag atcaaagcgg aaaccatcga cgtggcaggt
     6241 ttctacccaa taacgcaggc aggcgctggc ataatccacc accgccggat gactcaaatt
     6301 gagcgtgttg ccgcaaccgg tccagttgtg ataatcgccg tcttctctta tccaataata
     6361 gctacggtta tcgatcccac gcagcgagaa taacgggccg tcgaggtcca gttccgcact
     6421 atggttgagc acgatatcaa gaatgacttc gatacccgct ttatgcagtg ctttgattgc
     6481 atcgcgaaac tcatccagcg ccgtttctgg cgagcaggca tacgccggat gcagcgcaaa
     6541 catcgccacc gggttgtaac cccagtagtt acttagcccc atgcgttgca gacgtggttc
     6601 actggcaaac tgcgccactg gcagcagttc cagcgcggta atgcccaatt gtttcaaata
     6661 gttgatcatc accggatgcc cgagggcttt ataagtgcca cggatctcga ccgggatctc
     6721 cgggtgcaag tacgttaatc ctttgacatg ggcttcataa atgatggtgc tgccccacgg
     6781 cgtgcgcggc ggggcgtcat cttcccagtc atagtgatca accaccacta cgcatttcgg
     6841 cgcaatggcg gcgttgtcgc gatagtcagg ttcattatga ccggcgtgca gcagcgggtt
     6901 atctttaaac tccccgtcaa tttgccgcgc gcaaggatca atcaacaact tcgccgggtt
     6961 aaagcgatgc ccctcggcgg gttgccaggg gccatgaacg cgataaccat aacgcaaacc
     7021 cgggcgcgca tccggcagat aaccgtgcca aatgtcgcca ctgttccctg gcaagtcata
     7081 gcgatgttcc tggccattgg cgtcaaagac acacagttct acccgctcgg catgagcgga
     7141 gaaaagtgtg aagttgacgc cctgaccgtc gtaatgcgcg ccgaggggag cgggtttgcc
     7201 aatggcgagt tgtgtcattc tgcctcccga accagccaga tagtggccag cggtggtagc
     7261 gtcaggctta gtgaatgctg acgaccgtgg ctggcaatct catcgctgtg taccgtgccg
     7321 ccattgcctg cattactgcc gtgatagtgt atggaatcgg tattgaggat ttcacgccat
     7381 ttacctggct gattaatgcc gaagcgataa tcatgacgcg gcaccggcgt aaagttactg
     7441 gcaacgataa tttcgttacc ctctttatcg cgacgcacaa agatcagcac cgagcgttct
     7501 ttgtcatcca ccaccagcca ttcaaagccg tacgggtcaa aatccagttc atgcattgct
     7561 ttatggtggc ggtaggtgag gttcagatcg cgcaccagac gctggacacc gtggtgccag
     7621 ttatcgccgc cttccaacag atgccagtcg aggctggcgt catggttcca ctcgcggccc
     7681 tgggcaaatt cgttccccat gaacagcagt ttcttgcccg gaaatgccca catccagcca
     7741 tagtaagcgc gcaggttagc gaatttctgc catgcgtcgc ccggcatgcg gtcgagaatc
     7801 gattttttac cgtggaccac ttcatcatgc gacaacggca ggacgaagtt ttcagtgtag
     7861 ttgtagagca tcccgaaggt cagtttatcg tgatgatact gacgataaat cgggtcgagc
     7921 ttcatgtagt ccagggtgtc atgcatccag ccgaggttcc acttgtacca gaagcccaga
     7981 ccgcccatat cctgcggacg agaaacgcca gggaaatcgg tagactcctc agccattgtc
     8041 accgcaccgg aaacctgctc accaagaata cggttggtat tacgcaagaa ttcaatcgct
     8101 tcaagattct cgcgcccgcc aaattcgttc gggatccact ccccctcttt acggctgtag
     8161 tcacgataaa tcatcgacgc caccgcatcg acgcgcagcg catcaatacc aaaacgttca
     8221 atccagtaaa gcgcgttacc gacgaggaag ttactgactt cacggcgacc atagttgtag
     8281 atcagcgtgt tccagtcctg atgatagcct tcacgcggat cgctgtgttc atacaagttc
     8341 gtgccatcaa attcggcaag cgcaaagtca tcagtcggga agtggcctgg cacccagtcg
     8401 agaatcacgt tcagaccagc tgcgtgtgcg gcatcaatga aataacggaa gtcgtcgcga
     8461 gtaccaaaac ggcgggttgg tgcatacagg ccggttggct gataacccca actgccatcg
     8521 aagggatgct cgttaatggg cagtagttcg aggtgggtaa agcccatcca tttagcataa
     8581 ggcaccagtt gatcggccag ctcgcggtag ctcaaccaga aattgttgtc ggtgtggcga
     8641 cgccaggaac ccaggtgaac ttcataaata gagattggcg catcaaactg attcgctttt
     8701 ttgcgctctt cagtctgtac aaccttttcc ggcagcccgc aaataagaga cgcggtttcc
     8761 gggcgcattt gcgcttcgaa ggcataaggg tcggacttca gacgcaagtt gccattggca
     8821 tcaatcatct cgtatttata gagctgaccg ttatgcgccc cagggataaa cagttcccag
     8881 atgccgctct ctttacgcag gcgcatcggg tgacggcgac cgtcccagta gttgaattgc
     8941 ccaaccaccg agacccgacg ggcgtttgga gcccagacag agaaacgcgt acctgtgacg
     9001 ccatccatag tatctgcatg cgcgcctaag gtttcatacg ggcgcaggtg agtaccttca
     9061 gataatagcc aggcatccat ttcctggatt agcggaccaa aacggtaagg atcatcaatc
     9121 aggttttgct gaccatgcca gacaacagcc aactgatagc ggaaaaaatt cttacgtcgc
     9181 ggaatgacgc cgctaaagaa tccccgtgag tcgagacact ccagttttgc gagtttgcgc
     9241 ccggttttcg gttcaatcac ccacacatcg gtagcgtcgg gtaaaagggc acggacttcc
     9301 agtcccgcgg tggttttatg cattcccagt acggaaaaag gatccgcaaa atggcctgca
     9361 attagcgcgt taatcacgtc tctatcgata cgatcggaca tgcttgtctt cctgttttat
     9421 tgtgtcaccc catccaattg gatttttgac ttctggttgt gacatttttt tgacctgaac
     9481 ggcgcagcac tctgtgcatc ctctctgcgt cgtcctcact tcaggtaagg ctgtgaatac
     9541 tcatgtattc agccaccctt aaagaatagc caatgctcta tttaactccc ggtaaatcat
     9601 gaaacatctg cgcttactcc tgtattacgc actaacaggg gcggcatcgc gccccagatt
     9661 taatgaataa agattacgcc agttgacgaa gcatccgacg cagcggctcc gcggcccccc
     9721 acagcagctg gtcgcccacg gtaaaggctg acaggaactc tggtcccata ttcagcttac
     9781 gcaggcggcc taccggcgtg gtcagcgtgc cggtaacggc agctggggtt agctcacgca
     9841 tagtgatttc ccgatcgttc ggaacgactt tcgcccacgg attgtgcgca gccagcagtt
     9901 cttcgacggt cggaatagac acatcttttt tcaatttaat agtgaatgcc tggctgtggc
     9961 agcgcaatgc cccgacacgc acacataaac catctaccgg aattacggaa gatgtgttga
    10021 ggatcttgtt ggtttccgcc tgccctttcc actcttcgcg gctctgaccg ttatcgagct
    10081 gtttgtcgat ccacggaatc aggctacccg ccagcggcac gccaaagtta tccaccggca
    10141 gctcaccgct acgggttaag gttgtgactt tgcgttcgat atcgagaata gcagaggacg
    10201 gggtcgcgag ttcatctgcc acatggccat acagatggcc catctgggtt aataactcac
    10261 gcatatgtcg cgcaccaccg ccggaagcgg cctggtaggt tgcaacggac acccaatcaa
    10321 caagatcatt ggcgaataaa ccacccaacg acatcaacat caggcttacg gtacagttac
    10381 cgccaacaaa agtcctgatg ccattattta atccgtcggt aatgacgtcc tgattgacgg
    10441 ggtcaagaat gatgatggcg tcatctttca tgcgcagaga cgatgctgcg tcaatccagt
    10501 aaccttgcca tccgctttca cgaagctttg gatagatttc gttggtataa tcgccgccct
    10561 gacaggtcac aatgatatcg agggccttta gcgcctctag atcaaaggca tcctgaagtg
    10621 tgccagtggt tccgccaaaa gacggcgcag cctggccaag ctgagaagta gaaaagaaga
    10681 cagggcgaat ggcgtcgaag tcgcgctctt caaccatgcg ttgcatgaga acggagccga
    10741 ccataccgcg ccagccgata aaaccaacat ttttcataag cgttttttcc tgcaaagatg
    10801 tgtgctgtat aaatgtgccg gtctcctctt ggcacatctt tcaccataca aaaagctgcc
    10861 aaagtcgcaa gtgaaattaa tcaatgatag cgaagccatc agtaatgcga cttatcctgc
    10921 tttgttagca cgcagaaagt ccgcggcaat tatcagggaa tttgagttat gaatgaaatc
    10981 atttctgcag cagttttatt gatcctgatt atggatccgc tcggaaacct gcctattttc
    11041 atgtccgtac tgaaacatac tgaaccgaaa agacggcggg caatcatggt gcgagagttg
    11101 cttattgctc tcctggtgat gctggtgttc ctgtttgcgg gtgagaaaat tctggcattt
    11161 ctcagcctgc gagcagaaac cgtctccatt tctggcggca tcattctgtt tctaatcgcc
    11221 attaagatga ttttccccag cgcttcagga aatagcagcg ggcttccggc aggtgaagag
    11281 ccatttatcg tgccgttggc aattccgtta gtcgccgggc cgactattct cgccacgctg
    11341 atgttgttgt ctcatcagta cccgaatcag atgggacatc tggtgattgc tctgctgctg
    11401 gcctggggcg gcacctttgt catcctgcta cagtcttcgc tatttttacg tctgctgggc
    11461 gagaaagggg tgaacgcact tgaacgcctg atgggattga ttctggtgat gatggcaact
    11521 cagatgttcc tcgacggcat tcgaatgtgg atgaaggggt aacgaggaga gtttttcgaa
    11581 aagagcccgg tagcgtcaca actaccgggc gaacaaactt aactcaacag ctggaacgca
    11641 atcatcccaa cgatggcacc aacagtgccg aggatggttt ccatcatggt ccaggttttc
    11701 agcgtttcgg cttcggtcgc gccggtaaat ttaccgaaca accagaaacc agcgtcgtta
    11761 acgtggctga caacaatcga accaccagcg atacaaatcg acagtgccgc catttgcgca
    11821 ccagagtagt tcagttgttc aatgactggc atcaccagtc ccaccgccgt taaacaggct
    11881 acggtggcag ccccctgaat gatgcgcact gcagcagcca gcacgaagca ggtgatagca
    11941 atcggcaggc ccatgccggt taacgcttcg cccagtgccg gacctacgcc agagtcaacc
    12001 agcacctgtt tgaacacgcc gcctgcacca atcaccagca gaatgatccc cgccggttgc
    12061 agcgcgtgac cgcaaatctc catcactttg tcttttggca tgccctgacg cattgccagg
    12121 ccataaatcg ctaccagaca agcaaccaga atcgcggtaa acggatgacc aataaactcg
    12181 aaccattcgt aagcggtaga gccttctggc acaaaacgcg cggcaatggt tttcagcccc
    12241 accagcacca gcggcaacag gatcagcgac aggctgaatc cgaaagatgg cattttacct
    12301 tcgccgagat gcggttcgct gatgtcgtca ggaatatgca gctcaacgta acggctgatg
    12361 aagttacccc acagcggccc ggcaataatc attcccggaa ttgccgcaca caggccaatc
    12421 aggatcatcc agccaaagtc ggcattcatc tgcgatgcca gcagcattgg cgctggccca
    12481 ggcaccagga acgctgccgc ggccgccacg cctgcgaata atgggattac cagcttcacc
    12541 aggttcgtac cggtgtggcg cgccattgag aaagcaacgc taatcagcag aacaatcgcc
    12601 acttcaaaga acagcggtag cgcacagacc agccccgcaa ggccgatggc ataatgcgcg
    12661 cggctgtgac cgaaggattt gagcattttg acggcaatct gatcgactgc gccggtttca
    12721 tataagatct tgccaaacat agctcccagg gcgacaacca ccgccaggaa gccgagggtg
    12781 cctcccatcc ctttttccat cgtcgctgcg attttatcga gcggcatacc ggaaaaaagg
    12841 ccagccccca tggacaccac catcaaagcc aggaaagcgt gcatacgcgc cttcatgact
    12901 aaaaacagca gcagtaaaac agaccctact gctgttaaaa caagcgttaa tgtagtcact
    12961 acttatttgc cttttttaat aacctcaatg gtgcttgcca caacaccttc cagcggttga
    13021 tcgatatcca ccaccagtac atcggtttcg tccgcacccg gctcctgcag cgtttcaaac
    13081 tgcgtcacca acatttgggt tttaaagaaa tggcctttgc gcgctttcag gcgactttca
    13141 atcacatcaa aatcgccttt caggtagatg aaagagagat tcggattacc ttcacgcagc
    13201 aagtcgcgat agtgcttttt cagtgcagaa caggcgatca gcgacacttt attggtgcgc
    13261 tgcatagcaa acgcggagtc gttcagcgcc tgcaaccagg gtttgcgatc gtcgtcattc
    13321 agcggttcgc cagacgccat tttttcgata ttgcgccgtg gatggaggaa atcgccatca
    13381 agaaacgcgg catgaagttg atgcgccact tcactggcga ccgcagattt gccgctgccc
    13441 gatacgccca tcaagacgta aatgtggtga tcatggttag tcgtgctcaa agtggtgccc
    13501 ccacaataca agaattaaaa atgttacggg taactgttat cggcaacatt gtccagccgg
    13561 acaatatcag aagcaatatc catccgtgcc ttaagtgtat aagtgtgagc tacttcaaat
    13621 ttgtgggctt aaatagatcc gcccggtgac aaggtgaaac ctaaatctaa cattttcggt
    13681 gtcacagatt cgccacgaat acgcgccagc aggcgttcag cgccaatact gcccatccgc
    13741 tcacgcggcg tcagcacgct cgcaagtcgt ggctccatca cctgaccaat gtcatgaccg
    13801 tggaaaccgg caatcgccat atcgtcagga acttttaacc ccagacgctg acattcaaac
    13861 gccgcgccga ccgccagatc atcattagta cagaacacgc catccagctg cggatattcc
    13921 cgccgcgcct ggcgaatcag ttcaataccg gaagagtaag aagaagattg ctcaaccatc
    13981 acgctatatg gcaccaggcc tgcatccagc atcgcctgtt cgtatccctt ctgtttgatg
    14041 atagtacgtt cgtcgagacg tgcgccgaga taggcaatgt ggcgatgccc acgagcaata
    14101 atggcggtag tcatctggcg tgccgcttca aagttatcaa aaccgacggc gatatcgagg
    14161 cacggcgact ggctgtccat caactccacc accgggatcc ccgccacttc aatcatcttt
    14221 aaggtgcgcg gcgtgtgggt acgttcggtg aggatcaggc cgtcgatatt ccaggagagc
    14281 atggattcga gacgttcttg ctccatttcc ggtttataac cgtagtgcgc cagcatggtc
    14341 tgataaccgt gcgcgtcggt gacgctttcg attccgcgta atacttccgc gaaaacctgg
    14401 ttggtgagag aaggtaacag gacgccaatc gcccggctgg tagcgttaga gaggatatcg
    14461 ggcgcacgat tgggaatata gcccagttca tcaagagcgg cggcaatctt gccgcgtaga
    14521 gcgacggaaa cctgctccgg gttgcgtaaa aaacggctga ccgtcatttt ggtcacgcct
    14581 acacggtcag ccacatcctg aagtacgggt cttttctttt tcatcgtcct gaaggtacaa
    14641 aagagataga ttctcttagt ttaacacgga cgcatcagaa ccttcccggg gaaaacagtg
    14701 cttgtttaca attattagat atacactaaa tcaattgcat cgcattgtgc taacgacgcg
    14761 gcaaaacccc ggaagcgtgc atcagcacgc gaccggagcg agcgaggaag cctgcaaaaa
    14821 ggcaggcttc aaagatgccg ttgagtttta aaccggcggc agatcgaaca gtaacacttc
    14881 gctgtcgcta tccgcatgga tggagattgc ctgctcatcc cagattgcca gaccatcgct
    14941 ggtcgaggct ttcacgccgt taatggtaac attgcctttt accacctgaa tccagacgcg
    15001 gcgttcagcg gcaatctgat gcaccgactg ctcatctttc agcaacgccc aacgatacag
    15061 ttccatatcc tgatgcactt tcagcgaacc atctcgcgca tccggcgaga gcaccagctg
    15121 tttgccctgc acggcatcga agcgacgctg ttcataacgc ggcgtaatgc cattttcttc
    15181 aggcatgatc cagatctgat acagatgcag acgctcggtg ctgcttgggt tgtactctga
    15241 gtgacgaata cccgtacccg cactcataat ctggaactca cccgccggaa cctgctcttt
    15301 attgcccatg ctgtcctgat gctcaacagt accttccagc acgtaggtca aaatttccat
    15361 atctttatgc gggtgagtgc cgaagccctg ccctacttca atcacgtcgt cgttaatcac
    15421 gcgcagcgcg gagaagccca taaagttcgg atcgtaatag ttggcacaag agaaagtatg
    15481 ccaggagtcc agccagccat gatttgcatg accacgttca tttgctttgc gtaagtagat
    15541 catttgtatt cgccccctga atgatttcga tgggcttagt gtggacccga tctgcctggg
    15601 atgatagagg gtgaaaattg ccccctctgt tcaaaaaaat tgaactattc gaggggccat
    15661 caaacttaat tcgcgagagt cactgtggag ggagaggctt gctcaaatcc gcgttcaagg
    15721 atttccagat tggtaagaac ttcagattcc ttgacgtaat ttggcgcacc gtgggtgatg
    15781 gtttgataca acgcatcata aacacgcccg taatcgccca tctccggctt catctcttct
    15841 ctgaccgtca caccctcgtc attgacatac tccagcacac cgaccgaatc atccgctgcg
    15901 aatcccggtt cgcccggcat aatattagcc ttcaggctgg tttcctgctg gtcgataccg
    15961 tatttaataa acgaaccttt cttaccgtga acgataaatt tcggataatc gattttcacc
    16021 agatggctgg ttttgacgat ggctttcagg tcgccataaa acagttgcgc ttcaaaggtg
    16081 tcgtcaggat tggctttatt acgcaggctg cggatgtcat aagcgacgtg atccgggcga
    16141 ccgaacagag aaataatctg gtccatcgta tgcacaccaa ggccatagaa cgcgccatcc
    16201 tgcggcagtc caggtttggt ttctgccacc gggcggtaat agtcaaaatg gctttccact
    16261 tcgacaatct ctcccagctt gccgctttca atcgcttttt tcgccgtcag gaagcaggaa
    16321 tcaaagcgac gattctgata tggcgtgacg gtcagccctt tgcttttcgc cagtgcaaac
    16381 agctctttcg cctgcgcaag tgtcggagtg aacgtttttt cgaccagcac atttttcccg
    16441 gcttccagcg cgcgcttcgc gtactcgaag tggctgtccg catgggtgca gacaacaacc
    16501 aacttaacat cgggatcgtt tagcacttcg tcgagatcgc tggtgaaatg gatatgagag
    16561 taaatgggag cctgttcttc cggcttcgca tggcgacgaa aaatatgcgc gacatgccag
    16621 ctatccttgc ggttaagtac atacggcaga tggtaacggg tggtgctttt gccgaagcca
    16681 ataaaggcgc agttgatgac catgatttcg tcctttttaa ggtggttatt tacaccttag
    16741 cgcaaagcgg acgtggttcc tactggagtg cgcataacga acacaagcac tcccgtggat
    16801 aaattgagaa cgaaagatca aaaaaaaagc cagcacccgg ctggctaagt aatactggaa
    16861 gcaatgtgag caatgtcgtg ctttcaggtt ctccgcgagg gtcttcctga tcgcgataca
    16921 ataataatca ttctcattcg cacttgtcca acactttttg caaaaaaatg catttgactc
    16981 gcatttgaaa gtcaatgatg ttgaaaggga catttacccc aaagaggaca aaggaatgag
    17041 tgagatagta atacgccacg cagaaacacg ggattacgag gccatcaggc agattcacgc
    17101 ccagccggag gtgtattgca acacactaca ggtgcctcat ccttccgatc atatgtggca
    17161 ggagcgactc gccgatcgtc ccggcatcaa gcaactcgtc gcctgtattg atggagacgt
    17221 cgtgggccat ctcaccattg acgtgcaaca gcgcccacgc cgcagtcatg ttgccgattt
    17281 tggtatctgt gtcgactctc gttggaagaa ccgcggcgtc gccagcgccc tgatgcgaga
    17341 gatgattgaa atgtgcgaca actggttacg ggtagatcgc attgaactaa ccgtgtttgt
    17401 cgataacgcg ccagcaatta aggtctataa aaaattcggc tttgagattg aagggaccgg
    17461 taagaagtac gcattgcgta atggtgaata tgtcgatgcg tattatatgg cgcgggtgaa
    17521 atagagaatt tgcaatctaa gtttgtcatt taagccctca cctaccggga gggcttaaga
    17581 tattaatacc ccgccgttaa atcatccacc gagcgcgggt cggatgcgcc gtacaactca
    17641 ccgtccggcc caaccataat gctttgtgtg ctgcccatcg cctctttcag cgccactttc
    17701 tgaccttttg cttccagcag cttgagcgta tccgggctaa accctttttc gacacgcagc
    17761 tcgtccggca accactgatg gtggaaacgc ggcgcattgg tcgcttcggc gacgttcatg
    17821 ccataatcga tgctattcac caccatttgc agcaccgtgg tgataatccg gctaccgcct
    17881 gggctaccgg taaccagcca ggttttaccg tctttcacca caatggtcgg cgacatcgac
    17941 gacagcgggc gtttgttcgg cccgacggcg ttggcatcac cgcccaccag cccgtaaacg
    18001 ttcggtacgc ccggtttggc ggagaaatca tccatctggt tattaagcag aataccgctc
    18061 tcacccgcga caatacccgt accgaaggtg gtgttcagcg tataggtcac cgccaccgcg
    18121 ttaccgtctt tatccactac agagtaatgg gtagtttgat tactctcata aggcgcaagc
    18181 ttgccagggc gaatctcact ggacggcttc gcttttttga tatcaatttg atcggcaatg
    18241 gatttggcat aggctttatt ggtcagtgcc tgccacggga ctttgacaaa atccgggtcg
    18301 ccaagatatt ccgagcggtc ggcgtaggcg tatttctccg cttctgccat gatttgcatc
    18361 gcatcggcgc tgccaaagcc gtatttcttc atatcgaagt tttccagaat attgaggatt
    18421 tgtacgatat ggatcccgcc ggaggatggc ggtggcatgg agtaaacctg atacccgcga
    18481 taatcgccgc ttatcggagt gcgttcgacc gctttatagg ccgctaaatc ttctttagtg
    18541 atcaagccac cgtttttctg catctcctgg gcgatctgtt ccgcaatcgt gcctttatag
    18601 aattcgtccg ggccgttttc agcaatcatc tccaggctct ttgccaggtt cgcctgcacc
    18661 agcgtgtcgc cctttttcag cggctcgccc tctttccaga agatagcttt actgttttcg
    18721 tgattcggca acacttcgct accgtaggtt ttgagatcgt cagccagcgc gtcgttaacg
    18781 ataaaaccat cgcgtgccag tttaaacgcg ggctgcacga ctttgttcag cggcatggtg
    18841 ccgtatttat ccagcgccag cgagaaacct gctaccgtac ccggtgtgcc ggaagccaga
    18901 tgcgaagtga gtgatttttt gctgtccggg ttgccctgat catcgaggaa catatcgcgg
    18961 gtcgctttgg cgggtgccat ttcgcggaaa tcgatagccg tggtattgcc atttttcgag
    19021 cggattaaca taaaaccacc accgcccaga ttccctgcct gcggatgcgt taccgccagc
    19081 gcgtagccca ccgccacggc ggcatcaacg gcattcccgc cctccttgag gatatccacc
    19141 cccacctgag tggcagtggc gtccacagac gctaccattc cctgtttcgc gcgtaccgga
    19201 tggaagacat cttcctccac accatacgag acgggcggcg caggaggcgc ggcggcggcg
    19261 ctaaaacaac ttcctgagag cagagcagca atggccaccc ggcgtaaaaa cgtcggtttt
    19321 atcatcgtta ttctccagag attaaggggc aaccccagct aagcctggta tataactctg
    19381 aattaatcat cgttttgtcg ggaagcgagt aaacttaaag gatatcttca gaggaggata
    19441 cgcgatgaaa cgacttctga ttcttacggc actcctgccg tttgtcggct ttgcacagcc
    19501 cattaatact ctgaacaacc ctaaccagcc ggggtatcag atacccagcc agcagcggat
    19561 gcaaacccag atgcagactc agcaaatcca gcaaaaaggg atgctgaatc agcaactgaa
    19621 aacgcaaact cagttgcaac agcagcattt agaaaaccag ataaacaata attctcagcg
    19681 ggtgttgcag tcacagccgg gggagcgaaa tcccgcccgg cagcaaatgc tgcccaacac
    19741 caacggcggg atgttaaaca gcaaccgtaa tccggatagt tcgttgaatc agcagcatat
    19801 gctgccggag aggagaaaca gcgacatgct gaatcagccc agcacgccgc agcctgacat
    19861 tccgttgaaa actattgggc cgtaaagttc ggaccaatca cgtcaatcgc atcggtacag
    19921 atgcaatcca cgccccagcg cagcaactct gccgcgtgct ggggtttatt gacggtataa
    19981 accagaatcc gtaatccggc gtctttcaac tgcatcactc gcgctttatc gagtaactta
    20041 tgattgagat gaatagagac gcagcccagc cgcgcggtca gttcgcgcca gtcgtcgcgc
    20101 cactcatcaa gcaataaacc acgcggcagt tccggtgccg cctgttgtgc agcttctaaa
    20161 gcatcaatct caaacgatga cagcagcggc ggcgtcatac cggcccacag ttggcgtgcc
    20221 gccagcgcca ccattttgcc cgttaatggt ccagtgccag tggtgggttt gatttcgata
    20281 ttcgccatca tcccgtgttc gcgacagcgt tccgccacct gcgaaagcaa cggcagcggc
    20341 tcacctttaa acgctttgct gtaccaactg cccgcatcca cgcgcagtaa atcctgccag
    20401 ttcagttcac ccgcgacgcc ccagccgttg ctggtgcgtt cgagattgtc gtcatggagc
    20461 aggaagatct cgccatcttt cgataacttc gcgtcaaatt cgatcatctt atgaccgtat
    20521 tttgccccga cgtcgattgc cgccagggtg ttttccgggg ccagcttacc gccgccacga
    20581 tgagcgacga tgcggggata aggccagtta ctcatactcg ttgtcctgtt tcaccatcaa
    20641 aaagatgcag ctgattttcc gccagatgca gccacagcgt gctgcctgcc gtcgggcgct
    20701 cctgatgcgc cagtcgcacc accagcttct gcttgcgagc ttagcgcaat atgttccggg
    20761 cgaatgccga gggtcatttt acgcccggca tactgacggt agccaccgtt tagcggtagc
    20821 acgataccgc cgtccagttc gaaatgcgtg ccttcattat tcatgcggcc tgtcagcagg
    20881 ttcatcgccg ggctgccgat aaaactcgcc acaaacaggc tggcgggctt ttcgtagact
    20941 tccaccggcg tgccaatctg ttcggcaacg ccgccgttca tcaccattac tcgctgggcg
    21001 agcgtcatcg cctcaacctg atcgtgagta acgtagagtg aagtcgtttt caggcgacgg
    21061 tgcagctgtt gcagttcaag acgcatctgt acgcgcagct tggcatcgag gttagagagt
    21121 ggctcatcaa acaagaacac cgccggatcg cgcacaatcg cgcgtcccat cgccacacgc
    21181 tggcgctgac cgccggaaag ctcgcgcggg cgacgtttga gcagaccgtc cagctccaga
    21241 atgcgcgccg cttctttaac gcgctcggct atttgctgct tgcccatgcc gcgaattttc
    21301 agcccccacg ccatgttttc ttcgacgctc atatgcggat aaagcgcgta attctggaac
    21361 accatcgcaa tcccgcgatc tttcggctcc atttcggtca cgcgctggtc gttgatccag
    21421 atatcgcctt cggtcaccct ctccagcccg gcaaccatac gcagcagcgt cgatttcccg
    21481 cagccagacg gcccgaccat cacgataaat tcgccatccg ccacatcaag ggtcagcggt
    21541 ttaatgacct aggttttgcc atcccagctt ttggttactg cctgtaattt cagtcctgcc
    21601 atcttatttc tcactatcga ccaggccgcg cacgaaggca cgctgcatca ctaaaacaat
    21661 cactactggt gggatcaggg ttaacaacat cgccgccatc actgagttcc attccgtggt
    21721 gccttcgcca gtggcgatca tccctttgat ccctgccacg gtggtgccga gatccacgtc
    21781 ggtaataatc aacaacggcc acaaatactg gttccagccg taaataaagg tgatcacaaa
    21841 cagcgccgcc agattagttt tggagagcgg aaaaacgatg tcgcaaaaga agcgcattgg
    21901 tgacgcaccg tcgatccgcg ccgcttccac cagttcatcc ggcagcgtca taaagaactg
    21961 gcgaaacaga aacgtggcgg tcgccgaggc catcagcggc agcgttaaac cggcgtagct
    22021 gtcgagcatc ttcaggttgg cgatgacttc caccgtcggg aagatacgta cttcaaccgg
    22081 cagcatcagg gtgataaaaa tcatccagaa gaagaggtta cgtagcggaa aacgaaacca
    22141 gacaatggca aatgccgaga gcatcgagac agtaattttg ccgagcgtaa tgctgaacgc
    22201 catcacaaag ctgttaagca acatccgcca gaacggcgcg ctatttgtgc ctaccccgtt
    22261 cacccagatg ttgtggatgt tttccagcag atgcgtgccg gggatgagcg tcatcggcgc
    22321 ggcgtagacg gcctgtttat ccagcgtcgc cgcgacaaac gccacgtaca gcgggaagag
    22381 gatcaccgcg atcccgagga tcagcatggt atggctgaat atcgtcagcc acggacggtt
    22441 ctcaatcatt ggtaacgcac cttgctttca acatagcgga actgcaccac cgtcagcacg
    22501 atgacgagga acatcagcac catcgactgt gcggcagacg aagccagatc cagtccggta
    22561 aaaccttcgc ggtagatctt ataaatcagc gtcgtggtgg cctgaaccgg cccgccggac
    22621 gtggcggcgt cgatcaccgg gaaggtgtcg aagaaggcat acaccaggtt cactaccagc
    22681 aggaagaaac tcaccggggc gataagcggc agcgcaatct taaagaagcg gcgaatcggc
    22741 cctgcaccgt cgatggctgc ggcttcgatc aacgaacggg gaatggattg cagcgcggca
    22801 tagaagaaca gaaagttgta gctgatttgc ttccacaccg aggcaaacac caccagaaac
    22861 atcgcctgac cgctattttg cgcgtggttc cagtcgtagc cgaactcagc gagaaaatgg
    22921 gtgatcagcc cgcgaccggg gttaaacagg aagatccaca atacggcggc aacggcggga
    22981 gccacggcat acggcagcaa cattaaggtt tggtagaaac gactgccgcg cacgatgtac
    23041 tccaccagcg ccgcaaagaa cagcgatacc agcaaaccgc tgacggtgac aaaggtgctg
    23101 aattttatcg tcgtccagaa ggagtcgaga taatagctgt catgaaacag cgtgacgaag
    23161 ttatccagac cgacaaactg gctggagaaa ccaaacggat cgacgctttg tagcgagtac
    23221 cacaacgctt cgcccgcagg ccagataaaa aagataacgg tgatgatgag ctgcggcgcg
    23281 accagcagat aaggcagcca gcgcgagcgg aacaccggac gggatgatga cattacggtt
    23341 aattcctgaa ctgtgccgga tgcgcttcgc ttatccggcc tacacgcgat gccaggtcag
    23401 ataagcgaaa cgcatccggc attacactga ttaagacttc gtcgatttct caaagcggcg
    23461 cagtaactgg ttcccacgct caactgcggt atccagcgcc tgctgtggcg tcttcttacc
    23521 ggtccacacg ctctcaagct cttcatccac aatcacgcgg atctgcggca tgttgcccag
    23581 acgaagccct ttggtgaacg gcaacggcgg cttgttcagc atctgacgcg tcgcggtatc
    23641 cgcacctggg tttttctcgt aaaagccctg ctcacgggtc aggtcatacg ccgctttggt
    23701 aattggcaga taaccggttt tctgatgcca ctcagcagcg ttttctggct tcgcgaggaa
    23761 gtcgagaaac ttcgccacgc cggtatacgt ttctttatct ttgccctgca tcacccacag
    23821 gctggctccg ccgataatgg cgttttgtgg cgcatctttc gcatcggcgt cgtaaggcat
    23881 catacctacg ccgtagttaa atttggcgta ctcgcgaatg ttggcaagag aaccggaaga
    23941 ggcggtggtc atcgcgcaat caccgttata gaacttctcg gtggattcat ccttacgacc
    24001 gacgtagctg aagtcgccct tcttgttcat ctcctcgagc atggcgatgt gtttcacctg
    24061 ctccggcttg ttgaactcca gcaccgcgtc cgtgccgtca aagccgttgt ttttgctggc
    24121 aaacggcaga ccgttccagg cgctaaagtt ttccagttgg atccagccct gccagccgct
    24181 ggcgtagccg cacttaatac cggaggcttt cagttttgcg gaatagtccg ccagatcctg
    24241 ccaggttttc ggcggctgtt ccgggtctaa ccccgctttc ttgaaggcgt ctttgttgta
    24301 atagagaacg gaagtcgagc tgttgaacgg ctgggagagt aagtggccgg ttttgctgtc
    24361 ggagtagtaa ccggaaaccg tcggcacaaa ctgcgactca tcgaactgaa tccctgcctc
    24421 tttaaacacg tcatacaccg gtttaatggc tttcgacgcc atcatggtgg cggtgccaac
    24481 ttcataaacc tgcaaaatag ccggagcgtt gccggtacga aatacggcaa tccccgcgct
    24541 taaattctgt tcgtagttgc ctttataggt cggtacaatt ttgtaatccg gattttcggc
    24601 gttaaaacgt tgggccagag aatccacctc tttacccagt tccccttcca tagaatgcca
    24661 gaacggaatg gtcgtcactg cctgtgcatt ccccattaac gccagtccga gcgccagtgc
    24721 tgaagctgta taatgtaacg gtttcatcgt ttatctctct tgttgtaccg aatgcgcgaa
    24781 ttcacgcgtt ttatgctcgc ggggtaacat gacatgctcg aattacagaa aaataacttt
    24841 tttgttacat ttgtaagata gtaaggtgtc agaaagatga caaggcggtg acggcgtggg
    24901 tgagggaaaa tgggagatgg ggcacggata agcggaaaaa tagaaggtct gaatcaaact
    24961 ctacagatta ctcatcgttt catgccggat gcggcgtaaa cgccttatcc ggcctacaag
    25021 atcgtgcaaa ttcaatatat tgcaactcac ccagtaggcc tgataagcgc agcgcatcag
    25081 gcaattttgc atttgtcacc tgtctcaaag gagtcttttg actccttatc aatcaacgtg
    25141 ttattacccg cctaaatacg cacttctcac tgcttcgttc gccagcagcg cgtcgccggt
    25201 atcggagagc accacatggc cgttttccag cacgtagccg cgatccgcca gctttagcgc
    25261 ctggttggcg ttctgctcga cgaggaagat agtcatcccc tgctcgcgca gctgctcgat
    25321 ggtgtcgaaa atttgctgga tgataatcgg cgcaagaccg agcgatggct catcaagtaa
    25381 caacaaacgc ggattgctca tcagcgcgcg accaatcgcc agcatctgct gttcaccgcc
    25441 ggacatggtg cccgcccgct gaacacggcg ctcatgcaaa cgtggaaaca gttcatacac
    25501 ccactttatg cgctcctgaa actggtcacg ttcggcaaag aagccgccca tcgccaggtt
    25561 ctcttccacc gtcatccgcg agaagacgcg acgcccttcc gggacaatcg ctaccgcttc
    25621 gcgcatgatt ttcgccgtct gccagtcggt aatgtcttta tcatcaaaca caattcgccc
    25681 gctggtggcg cgcggatcgc cgcataacgt gccgagcaag gtggttttcc ccgccccgtt
    25741 cgcgccaatg agagtgacaa tctcgccctg attgatatgc aggctcacct catgcagcgc
    25801 ctggattttg ccgtagtggg cgctgacttt gtcaaaggac aacatgactt tttccatctt
    25861 atgcctcacc taaataggca cggatcacgt ccgggttatt acggatttgc tccggcgtac
    25921 cgtttgccag tggcgtcccc tgattgacca cataaattcg gtctgaaatc cccatcacca
    25981 gcttcatatc gtgttcaatc aacaaaatag tggtgttgtg atgattgcgc agttcggcaa
    26041 tcagctcatc cagctctttg gtttcttttg ggttaagacc tgccgccggt tcgtcgagca
    26101 ttaaaatctc cggctgcgtc accatgcagc gggcaatctc cagacggcgt tggtcaccat
    26161 aggccaggtt actcgcctga cggttggcgt gttccagcaa accaatgcgc tcaagccagg
    26221 tggcggcgcg gtcgagcgct tcgctctggg cgcgacggaa agaaggcgtt ttcaacaggc
    26281 cagagaacag cccggttttc agttgctgat gctgcgccac cagcaggttt tcaattaccg
    26341 tcatttcacg gaacagacgc acatgctgga aggtgcgcac cacgcccatg cgagcaattt
    26401 gctgccccgg taaaccttca agatgctgat cgcgcagtaa aatggtgccg ccggtgggtt
    26461 tgtagaaacc ggtcaggcag ttaaaaaccg tggtttttcc ggcaccgtta gggccgatta
    26521 acgagacgat ttcctgcggg tacagttcaa gattgacgtt gttcaccgcc agcaggccgc
    26581 cgaagcgcat catcagaccg ttaacagata ataatggctg actcatgcct gctctccttt
    26641 cgctgcgccg tttttcagct tcagttgcgg gcgcgtcatg ggcagcaaac cctgcggacg
    26701 ccagatcatc atcagcacca tcaaaccacc gagcattaac atgctgtatt cgttgaaatc
    26761 acgcatcaat tcgcgcgaca ccaccagcag aacagccgcc agaatcactg caaattgcga
    26821 gcccataccg ccgagcacca ctatcgccag cacaaaggcc gattcagcaa aggtgaagga
    26881 ttccgggctg acaaagccct gacgcgccgc aaacagcgtt ccggcaaaac cggcaaatgc
    26941 ggcgctgatg gtaaaagcag tcagcttgat acggcgcggg cttaagccca gcgaacgaca
    27001 ggcgatctcg tcttcacgca acgcttccca cgcgcggccc agcggcatcc gcagcaggcg
    27061 gttaatgaca aacaggctta acaccaccag cagcaacgcc accaggtaga ggaagataac
    27121 gcgatcggac ggatcgtatt tcaggccaaa gaaattactg aacgtgtccc agccgccttc
    27181 acgagcggta cggctgaact cgagtccgaa gagtgtcggt ttcgggatct gactgattcc
    27241 gttcgggccg ccggtgattt cagtgttatt gagcagcaat atgcgcacaa tttcgccgaa
    27301 accaagggta acgatcgcca gatagtcacc gcgcagacgc agcaccggaa aaccgagcag
    27361 gaaacccgcc gccgctgcca tcagtccggc aatcggcaaa caggtccaga accccagacc
    27421 gtaatagtga ttgagcagcg caaaagtgta agcgccgatg gcgtaaaaac cgccgtaccc
    27481 cagcaccagc agaccagaaa gaccaacaac cacattcagc ccgagaccga ggataatgta
    27541 gatcatggtc agagtggcga tatccaccgt cccacgtgac accataaacg gccacgccac
    27601 cacaagcacc agcagcgcca cgaggaacag cttttgcttc accgtggacc catcaatcgc
    27661 gggcagaata aacttcggcc cggaaacgct tttcaaccct ttctggaaag ccggtcgcaa
    27721 aagctggaag aaaaagacca ccgccgtgcc gataaacatc cactgccaac ggatgtctga
    27781 agccgtgtcg accaccagtt tggtgccatc cagctccagt tgtacgccca taaagacgcc
    27841 cgccaggaca aagaacatcg cggcagagag cagtgccatt gcaatatgca tcggtttcat
    27901 actttctcta cctccgggcg acccagaata ccggtcggca tcaccagcag caccagaatc
    27961 agcagggcga atgagaccac atctttatat tccgtactca gataggcaga agagagcgcc
    28021 tccgcaatcc ccaggatcag cccgccaatc atcgcccctg gaatgctgcc aatcccaccg
    28081 agcaccgcag cggtaaaggc tttcatcccg gccataaagc cgatataggg gttaatgacg
    28141 cagtagaact gaccgagcag tacgccagcc actgccgcca tcgccgcgcc aataacaaag
    28201 gtcaaagcaa tcacccggtc ggtgttgatg ccaagcaggc tcgccatttt cagatcttcc
    28261 gcgcaggcac gacaggcgcg ccccatacgg gaatagcgaa tgaaaatcgt cagcgccagc
    28321 atggcgagga aggtgacaat ccagatcacc gcctgcatgg tggtaataga ggcagagaag
    28381 ttttcgctat ggcccaccac ccactgaccg ttaaacaggc tcggcagcgc cacgtcgcgc
    28441 gaaccttcgg tcaggctgac gtagttttgc aggaagatgg acataccgat tgcggaaata
    28501 agcgcaatca ggcgcttaga gttacgcacc gggcggtaag ctacccgttc gatactccag
    28561 ccgtaggcgc tggcgatgac gattgcgccg acgaatcccg ccgccaccag cagccagccg
    28621 gtatcaatgc ccatcatcat cagcgcggcg atgatcataa atgagacgta gctgccaatc
    28681 atataaacct cgccgtgggc gaagttgatc atgccgataa tgccgtaaac catggtgtag
    28741 ccgatggcta tcagcgcgta ggtactgccc agcgtgacgc cgttaaacat ctgctgcaag
    28801 aaatacaaaa actgctcaga cataaggtaa cctttctaaa cccgcccgca ttttacgggc
    28861 ggtgggatga tcacttggct gccgtggatg aaccgtcggc gtgccactgg aagacaccaa
    28921 aatcaaatcc cttaagatcg cctttttcat cccagttcag cggcccaatc acggtgtttg
    28981 caccgttagc ttttaaatct ttcaccagcg ccagcggctc atcgctgccg gtacgctcaa
    29041 gggcagttgc cagagattgc actgccgcgt aagtgatcca gacataaggc ccggacggat
    29101 ctttcttgtc tgctttcagc gcatcaacga tgccctggtt tgccggatcc tggtcatagc
    29161 gttttggcat agtgaccaac atgccttcgg cggcatcacc ggcaatgttc gacagcgatg
    29221 cgttaccgac accttccggc cccataaact gggttttcag gccaacggaa cgggcttggc
    29281 gcagcatctg ccccatttcc gggtagtaac cgccgtagta aacgaagtcg atattttctt
    29341 ttttcaggcg ggcgatcagc gcggagaaat ctttctcccc ggcggtaata ccgtcgaaga
    29401 agacgacgtt ggcgttagcc gctttcagcc cgtcctgcac cgaacgcgcc agcccttcgc
    29461 catactgttg tttgtcgtga atgatggcga tgcgctgggg cttcaccgtc tcaagaatgt
    29521 attttgccgc cgtaggcccc tgggaagagt ccagcccggc agtacgcata atgtgttgat
    29581 aaccgcgttg ggtcagctcc gggttggtcg ctcccggcga gatcatcaga ataccttcgt
    29641 cttcgtagat atccgacgct ggctgggtag aggaagaaca cagatgacca ataacgtatt
    29701 taatgccgtc gttaacgatt ttgttggcga ccgcaacggc ttgtttcggg tcgcaggcgt
    29761 cgtcatattc cacgccaacc agcttgtcgc ccttaattcc ccctttgaca ttaatgtctt
    29821 taatcgcctg acgcgcgccg ttaaattcca tatcgcccca ctgggcaatc gggccggaca
    29881 tcgcgccgac aacggcgact ttaatatcgt cagccatagc ggtgtgtgaa attgccagtg
    29941 caatcatccc tgcgatgata gttttcgcat tccgtttcat agtcaaaaat ccccattcgt
    30001 gatgttgtgt tgctttgttt ttatgtgtta acaaatcaga ctgttctttt tttatactgc
    30061 actgtttttg cctgtctgat taaggggtta gcgcagtatt ttgtgatgat agcgattaaa
    30121 atccctattt ttcagtcgat taagaacaga taatattctg aatttattga tagataaaca
    30181 gaaaaaagtg cctttgtcag cataaaataa cggcacaaag ggcggaataa ttcactatca
    30241 ttcaggggat tatgctggac atttttcatt ctctaatgtt ttaactttgt aattattgct
    30301 gttaaaaaat taatcacctg ccaaaagaaa taaaaaagag aaagcctccg attaaattat
    30361 ttcgctacac tggttccact tttgtgattt acacgggtta cccatgaagc tgaccatcat
    30421 tcgattagaa aactttagcg accaggaccg gattgacctg caaaagatct ggccggagta
    30481 ttccccttcc tctttacagg ttgacgataa ccaccgtatc tacgccgcgc gttttaacga
    30541 gcgtctgctt gcggccgtgc gggtaacctt aagcggcacc gagggagcac tggattctct
    30601 acgcgtgcgg gaagtcaccc gccgtcgcgg tgtggggcaa tatctgctgg aagaggtttt
    30661 gcgtaacaat cctggcgttt catgctggtg gatggcggat gcaggcgtgg aagatcgcgg
    30721 tgtgatgacg gcgtttatgc aggcactggg atttacggca cagcagggcg gctgggagaa
    30781 gcgttaatcg tcaagttgat ttcaaaagtg atgaccaacg agaaattgcc tgatgcgcta
    30841 cgcttatcag gcctataatg tgtgctgcaa tttactgatt tctttggttc ttgtaggccg
    30901 gataaggcgt ttacgccgca tccggcatga agcaacgtat tcgatattag caatttggcg
    30961 gcaacccaaa gttgccgatt aacaattact tcgcatcggt cgccgtgccg ttggcgtgcc
    31021 agtcaaatac gccgaactca aagcccttta gatcgccttt ctcatcccag gtcagtggcc
    31081 ccatcacggt atccacggag ttcgctttta ggtatttggc gatttcagcc ggatcgtcag
    31141 actgattcag gcccgcctgc aaagattgca gcgcggcgta ggtggtccag acgaatgcgc
    31201 cgcttgggtc ctgttttttc gctttgatcg catcaacaat gggtttgttc gccggaacct
    31261 gatcgtagtt cttcggcttg gtcaccagca gcccttccgc tgattcgccc gcaatgttag
    31321 acagcgaaac gttagccaca ccttccggcc ccataaactg agttttcagc cctgccgcgc
    31381 gtgcctgacg caggatttgc cccatttccg ggtgataacc gccgtagtaa acgaagtcga
    31441 tattctcttt tttcagacgc gccaccagcg ttgagaaatc tttttccccg gcggtgatac
    31501 catcaaagaa caccacgtta gcattgcctt tcttcaggcc gtcctgcacc gctcgtgcca
    31561 gaccttcgcc gtattgctgt ttgtcgtgaa cgatagcaat acgctgcggt ttcactttct
    31621 caagaatata tttcgccgcc gtcggcccct ggtcggagtc caggccggtg gtgcgcagga
    31681 tcagctgata gccacgggcg gtcagctccg gcgcggttgc cgctggggtg atcattaaaa
    31741 tgccttcgtc ttcgtagatg tcagacgcag gctgcgttga tgaagaacag aggtgaccaa
    31801 tcacatattt aatgccgtcg ttaacgactt tgttcgccac cgcaaccgcc tgtttcgggt
    31861 cacaggcatc gtcatatttt acgatttgca gtttgttgcc tttaatgccg cctttagcgt
    31921 tgatatccgc aaccgcctgc tctgcgccgg taaactcctg gtcaccgtac tgcgcaaccg
    31981 gaccggacat tgcacccacg accgcgactt taatatcttc tgccagagcc atattgctga
    32041 atgccagcgc gatacatcct gccagtaacg ctttaccctt tatgttcatc ctgagaatcc
    32101 ccattcttct ggttattacg tgtgttgtga tgttgttgtt cagcacttta tttcgtttta
    32161 tgcatgacta cccgtgcttt agcagcatac tctgctaaaa catacccgat ttttatgata
    32221 ttggaatagc tattttgaca gtttattaac aatctgcgtg gggattggcg ttttgccgga
    32281 gggggaattg attattgtag aggcccaaaa aacaaaaacc ccggactctc atccagggtt
    32341 ctctgcttaa tagcggaaat tacgcttcaa tagcggcgcg aagttttttc atcgcgttct
    32401 tttccagttg gcgcacacgt tcagcggaaa caccgtaacg gtcagccagt tcctgcaacg
    32461 tggacttgtt gtcttcgtcc agccagcgcg cacggatgat gtcctggctg cgttcgtcca
    32521 gaccatgcat cgcgtcggtc agacggtttg ccgcctgctc ttcccagtta tcatcttcga
    32581 tgccgtcggc aaagttagat gatttatcct gcagatagag caccggtgcc atcggctggc
    32641 tgtcggaatc gtcgtcggaa gacaggtcaa aggtcatgtc ctgtgccgcc atacgtgatt
    32701 ccatctcacg tacgtctttg ctggttacgc ccagttcacg ggccaccatt tcgacttcat
    32761 cctggttaaa ccagcccaga cgctgcttgg ttttacgcag gttgaagaac agtttgcgct
    32821 gcgctttggt ggtcgcaact ttgacgatac gccagttacg cagaacgtat tcgtggatct
    32881 ctgctttgat ccagtggacg gcgaaggaga ccaggcgcac acccacttcc gggttaaaac
    32941 ggcgcactgc tttcatcagg ccgatgttac cttcctgaat caaatccgcc tgtggcaggc
    33001 catagcccgc ataattacga gcaatatgaa caacaaaccg caggtgagac aggatcagcg
    33061 ttttagctgc ttccagatcg ccatggtaat gcagcttttc agccagcgcc cgctcctcgt
    33121 cagccgacaa catcggccac gcgttagctg cccggatgta ggaatccagg ttgccaactg
    33181 gggctaaagc taaactttgc attttgtcag tcattcaaat cctctcaatc gatatcttct
    33241 ggcgcttcag tggtagcaac aactgtgcca aagcttaaga gcaacgaggt tatcattcac
    33301 tgttttatca gaccgtgatt ttatccacaa gttcaatgca agcttgtgaa taaattacgc
    33361 acaaaatgtg acatagagat gaaataccgg gaagagacaa cggggtctct ttccctgcta
    33421 cggaacccat tgcagggaaa gagtataaca cgcttttatt attcaggcgt aaagtggcgt
    33481 aaatgttgta ccgtggcaag ccacgctgcc acccagccaa tcatcgagca taccagcagc
    33541 aatagcaggc attcatcgaa cgataaacca ttgatatcaa acttcgttcc gaaaacctgt
    33601 gccacttccg ccaccgctga cgacaaacgc agtaccagaa tttctgacaa aattaatgac
    33661 aacaatgcgc cagaaaatcc cagcagcgca ccgccgtaca ggaacgggcg aaggatgaaa
    33721 ccatccgtcg caccaatcag tttctgcacg ttgatggagt cacggcgagc aaagatgctc
    33781 agacgtacgc tgttgccgat gacgaggaac actgccgcca ccatcaacac gccgatcatc
    33841 gccgaaacgc gcccaaccag cccggtcaac gccgccagac gggcaaacca gctgtcatcc
    33901 atccgcactt cgtcaatacc gttaatctgc gtgatacgat cacgcagcgt attgagtgat
    33961 tccgtcccct gaaaatcgag tttcgggatc accaccgcca ctgccggaag cgggttttct
    34021 tccagcatat ccagcgcacc accaaaacca gaccagttac ggaactcgcc cagtgcgtct
    34081 tcacgagaaa gatagttcac tttctccacg ccttgctcgg cctgcaactg tgccaccacg
    34141 cccgcagcgg cgtcatcgtc cagcgttttt tgcaagtaaa cagtgatttg cggtgacgga
    34201 taatactgcg tcgccgcctg attaacgttt ttgtacacca tataacagac gctgggcagc
    34261 gtcagggaga tggcgataac catcaccgtt aaaaacgtgg cgaacggttt gcttttcaga
    34321 tcctgcaatg cgccgtggaa ggcatagcgc acctgttcgt tgaaaacgtt ggttttgcga
    34381 tttaccggtt ttggcgagga tttcgcgcgt ttcggtgcgt tacgaccgcc gtcgcctgag
    34441 ccgccgaccg atttacggaa gcgatccaga cgcccaccaa actgccgaat atgattgatt
    34501 gcatcgcgct tattcatggc ccacgcctcc atgcaagtga ccatcgctca gggtgagcat
    34561 gcgataggaa cgccgtgaaa tcagattaat gtcgtgcgtt gccatcaata cggttacccc
    34621 aacgcggtta aactcttcaa acagacgtag aatcccttcc gacaacgcgt cgtccaggtt
    34681 accggtcggt tcgtccgcca gcagtaccgc gggcttattc accaccgcgc gggcaatgcc
    34741 aacacgctgt tgttcaccgc cggaaagctg aatagggaag ttcttcgctt tgtccagtag
    34801 cccgacttta tccagtgccg ccgacacccg gcgacgaata tcgtcaccgc tggcaccagc
    34861 gataatcagc gggatcgcca cgttatcgta gacagtacgg tccatcagta gatggtgatc
    34921 ctggaaaatc atgccaatct ggcggcgcag aaacggaact tcacggtttt tcagacgcgt
    34981 gatgtcatgg ccgctaaacc agattttccc ggcgctgggc cgctcaatcc cacagatcag
    35041 cttcaggagg gtacttttcc ctgcgccgga atgaccggtc agaaacgcca tctcacccgg
    35101 ctgcatatgg aacgtaacgc cctgcagcgc ctgtctccca ccgagataag ccttgctgac
    35161 atgttcaaag cgaatcattg ttaatcctct cgggcaaaaa gtgcctctat aaagtcgtcc
    35221 gccttaaacg gacgcaaatc ctcaatacgt tcgccgacac caatgtagcg gatagggata
    35281 ccaaactggt cagccaccga gaaaattacc ccgcctttcg ccgtgccgtc cagtttcgtt
    35341 agcgtgatgc cggttaagcc aacagcttcg tggaacagtt tggcctggct taccgcgttc
    35401 tgcccggtgc tggcatcaat agtcagcata acttcatgcg gcgcttcaac gtcgagtttc
    35461 ttcatcacgc ggacgatttt cttcaactct tccatcagat gcgatttgtt ctgcagacgt
    35521 ccggcggtat cggcaatcag gacgtcgata ttacgcgctt tagctgcctg aatggcgtcg
    35581 aagataacag aggcggaatc cgcaccggta tgctgggcaa tcaccggaat attgttgcgc
    35641 tgaccccaga cctgaagctg ttcaaccgca gctgcacgga aagtatcgcc cgccgccagc
    35701 atcaccgatt taccctgctg ctcaaactga cgcgccagct taccaatcgt cgtggtttta
    35761 cccacaccgt tgacgcccac catcaggatc acgaacggcg ttttatcttc aacattcagc
    35821 ggctcatcga ctttcgccag aatctcgccc atctcttctt tcagcaggcc atagagcgcc
    35881 tcggcatcac gaagctgctt gcgggatgcg ccttccgtca gattggtgat aattttacgt
    35941 gtggtttcca cgcccacatc ggcgatcaac agctgttctt ccagctcttc aaacagatca
    36001 tcgtcgattt ttttaccgcg gaacaggctg ataaatccgg aaccgagatt ttctttggtt
    36061 tttaacaggc tgcgtttcag gcgcgcgaag aagccttctt tggtcggttt ttcttgctcc
    36121 tgggcaattt ctgcaaccgg ctgctcttct tctgccggag gaactaccat caccgcctct
    36181 tctgccgctt cggcagccag cgcctgtgct tccagctctt cgtcggtgat ttcttcttta
    36241 gccgcttctt cttctgccgc ttcgacaatc tctacggttt ccgcttcagc ctgccactct
    36301 tctgacgaaa cctcttcggc gttgacgtct tccggcaacg gcagctcttc acgttcgata
    36361 gccactggct ccggcgtttc ttctacgacc ggttccggct gtgcaacaac ttccgcttca
    36421 ggctgcgctt tttcactttc aacaacctgt tcagtgactt ccacaacgtc ggcagcaaaa
    36481 gtttccgcct cggcttcagt atgcgcctgc ggctgctctt caacggcttg ttcagaagcc
    36541 ttcgcaggct cttgcgcctg aacgatttct tctacaaccg gttgttcatt ctgaacttct
    36601 gtctcttttt ccggggtctg ctctttttga ccaaagccca gccaggaaaa aaagccacgt
    36661 cttttttctt tcgccatttg cgactacact cctcgctgtt gcttcatggc acagcgttaa
    36721 cgctatgtac atagcagcta aaaaaatgat gaaatagtct atcacttaac ttaattcaca
    36781 tcaccgcctg caagtatgtg ttatctggcg gattgagcaa tttatcatga aaaaaccgaa
    36841 tcattccggc agcggccaaa tccgcattat tggcgggcag tggcgaggcc gtaaactccc
    36901 ggttcctgat agcccaggtc tgcgccccac caccgaccgc gtacgcgaaa cgttgtttaa
    36961 ctggctggct ccggtcattg ttgacgccca atgtctggat tgcttcgccg ggagcggcgc
    37021 gctggggctg gaagcgttat cgcgctacgc tgcgggggca acgttgattg agatggatcg
    37081 cgtggtttct cagcagttaa ttaagaatct ggcgacacta aaagcaggca atgcacgcgt
    37141 ggtgaacagc aacgcaatgt cattcctggc gcaaaaaggc acaccgcata atatcgtgtt
    37201 tgtcgatcca ccgttccgcc gtggcttgtt agaagagacg ataaatttac tggaagataa
    37261 cggctggctg gctgacgaag ccctgattta tgtcgaaagc gaagtcgaaa acggtctgcc
    37321 cactgttccg gcaaactggt cattacatcg ggaaaaagtg gcgggtcagg tggcttatcg
    37381 gctgtatcaa cacgaagcac aaggagaaag tgatgctgat taatattggt cgtttgttaa
    37441 tgctctgcgt ttggggattt ttaatcctca atctggtgca tcccttcccg cgcccgctaa
    37501 atattttcgt taacgtggcg ctgattttta ccgtgctgat gcatggtatg cagctggcgc
    37561 tattgaaatc cactttaccg aaagatggcc cgcagatgac caccgccgaa aaggtgcgga
    37621 ttttcctttt cggcgtgttt gaactgctgg tctggcagaa gaaatttaaa gttaaaaaat
    37681 aaccttattg ttcgccaaca aagctaacaa agcgcgttcc tttatagctc agcgtacctt
    37741 tatcgcccac tgtcagggcg tggtactgct gggcgtcgag gcgaaacgtc tgctccattc
    37801 ctccgctttg cggtttgaag cttgcctcat agcgcatact ggtgcctgcc ggagtcactt
    37861 cctgctggcg cgaacggcga tcgttaatcg gtttttcccg cttgttgctc accaccacca
    37921 gcttttgctg gagcggagcc atatcattat cagctttttc ccgtcgctgt tgcataaaac
    37981 gaaacgatgc ggcgacgaca attaagccaa tgataacaat aaagaaaaga ggtggtttac
    38041 tcatctttat ccctcatcgg aaaatgcgga attaagcata ccctgccagt tatggtgttg
    38101 tcatccgtcc accctcgcca ctaaactgga agcaagaccg taggcattct gcttacgaaa
    38161 aaataacgaa ttcaaggaac taagatgctt tggtcgttta tcgctgtctg tctttccgca
    38221 tggctatctg tggatgcatc gtatcgtggg ccaacctggc aacgctgggt gtttaaaccg
    38281 ttaacccttc ttctcctgct gttactggcc tggcaagcac cgatgttcga cgccattagc
    38341 tatctggtgc tggcgggact gtgcgcctca ctgctgggcg atgcgctaac cctgttgcca
    38401 cgtcaacgtc tgatgtacgc catcggcgcg tttttcctct cgcacctgct gtacaccatc
    38461 tatttcgcca gtcagatgac gctctctttc ttctggcctc tgccactggt gttgctggtt
    38521 ctgggtgcgc tgctactggc gattatctgg acgcggctgg aagagtaccg ttggcctatc
    38581 tgcacgttta tcggcatgac gctggtgatg gtgtggctgg caggtgaact gtggttcttc
    38641 cgtccgaccg ctccggcgct ctctgcgttt gttggcgctt cgttgctgtt tatcagtaac
    38701 tttgtctggc tggggagcca ctatcgccga cgcttccgtg cggataacgc gattgctgcg
    38761 gcctgctact ttgccggtca cttcctcatc gtccgctcac tgtatctctg ataaaacttg
    38821 actctggagt cgactccaga gtgtatcctt cggttaatga gaaaaaactt aaccggagga
    38881 tgccatgtcg acacctgaca atcacggcaa gaaagcccct caatttgctg cgttcaaacc
    38941 gctaaccacg gtacagaacg ccaacgactg ttgctgcgac ggcgcatgct ccagctcgcc
    39001 aacgctctct gaaaacgtct ccggcacccg ctatagctgg aaagtcagcg gcatggactg
    39061 cgccgcctgt gcgcgcaagg tagaaaatgc cgtgcgccag cttgcaggcg tgaatcaggt
    39121 gcaggtgttg ttcgccaccg aaaaactggt ggttgatgcc gacaatgaca tccgtgccca
    39181 ggttgaatct gcggtgcaaa aagcgggcta ttccctgcgc gatgagcagg ccgccgacga
    39241 gccacaagca tcgcgcctga aagagaatct gccgctgatt acgcttatcg tgatgatggc
    39301 aatcagctgg ggtctggagc agttcaatca tccgttcggg caactggcgt ttatcgcgac
    39361 cacgctagtt gggctgtacc cgattgctcg tcaggcatta cggctgatca aatccggcag
    39421 ctacttcgcc attgaaacct taatgagcgt agccgctatt ggtgcactgt ttattggcgc
    39481 aacggctgaa gctgcgatgg tgttgctgct gtttttgatt ggtgaacgac tggaaggctg
    39541 ggccgccagc cgcgcgcgtc agggcgttag cgcgttaatg gcgctgaaac cagaaaccgc
    39601 cacgcgcctg cgtaacggtg agcgggaaga ggtggcgatt aacagcctgc ggcctggcga
    39661 tgtgattgaa gtcgccgcag gtgggcgttt gcctgccgac ggtaaactgc tctcaccgtt
    39721 tgccagtttt gatgaaagcg ccctaaccgg agaatccatt ccggtggagc gcgcgacggg
    39781 cgataaagtt cctgcaggag ccaccagcgt agaccgtctg gtaacgctgg aagtgctgtc
    39841 agaaccgggt gccagcgcca ttgaccggat tctgaaactg atcgaagaag ccgaagagcg
    39901 ccgcgccccc attgagcggt ttatcgaccg tttcagccgt atttatacgc cagcgattat
    39961 ggccgtcgcc ctgctggtaa cgctagtgcc gccgctgctg tttgccgcca gctggcagga
    40021 gtggatttat aaaggtctga cgctgctgct gattggttgc ccgtgtgcgt tagttatctc
    40081 aacgcctgcg gcgataacct ccgggctggc agcagcagcg cgtcgtgggg cgttaattaa
    40141 aggcggtgcg gcgctggaac aactaggtcg tgttacccaa gtggcgtttg ataaaaccgg
    40201 tacgctgacc gtcggtaaac cgcgcgttac cgcgattcat ccggcaacgg gtattagtga
    40261 atctgaactg ctgacactgg cggcggcggt cgagcaaggc gcgacgcatc cactggcgca
    40321 agccatcgta cgcgaagcac aggttgctga actcgccatt cccaccgccg aatcacagcg
    40381 ggcactggtc gggtctggca ttgaagcgca ggttaatggt gagcgcgtgt tgatatgcgc
    40441 tgccgggaaa catcccgctg atgcatttgc tggtttgatt aatgaactgg aaagcgccgg
    40501 gcaaacggtt gtgctggtag tacgtaatga tgacgtgctg ggtatcattg cattgcagga
    40561 taccctgcgc gccgacgcta caactgccat cagtgaactg aacgcgctgg gggttaaagg
    40621 agtgatcctc accggcgata atccacgcgc agcggcggca attgccgggg agctggggct
    40681 ggagtttaaa gcgggcctat tgccggaaga taaagtcaaa gcggtgacca agctgaatca
    40741 acatgcgccc ctggcgatgg tcggtgatgg tattaacgac gcgccagcga tgaaagctgc
    40801 cgccatcggg attgctatgg gtagcggcac agacgtggcg ctggaaaccg ccgacgcagc
    40861 attaacccat aaccacctgc gcggcctggt gcaaatgatt gaactggcac gcgccactca
    40921 cgccaatatc cgccagaaca tcaccattgc gctgggactg aaagggatct tcctcgtcac
    40981 cacgctttta gggatgaccg ggttgtggct ggcagtgctg gcagatacgg gtgcgacggt
    41041 gctggtgacg gcgaatgcgt taaggttgtt gcgcaggaga taaggcaaac cgatcgcaac
    41101 attgagcgcg atcggtcccc tcgcccctct ggggagaggg ttagggggtg aggaaaaggc
    41161 ggcatcgaag ccaatcagcc cctatcaacc gcctttacga atcaaataac gataaggcag
    41221 tccatccgtc tctttcgcaa ccagttcgtg ttccataaag gtacaaaacc caggaatatc
    41281 gcgggtagtg gccggatcgt cggcgataat cagcaacgtt tcgccaggct gcatattgcg
    41341 cacggttttg cgcaccatca tcaccggttc cgggcagcgc aggccaagcg cgtcgagtgt
    41401 gtggtcaggg ctggaaaaga gatcggtcat tttcttctca tcacttaaaa aaacggcgct
    41461 agtttacgcc ctgtgagtcc gtaagcaaac caggttaacg attgcgtgaa aattaaccat
    41521 tgcattgtca acgtaaagca gtatcatgcg gcggctcgaa aaaagggtaa gcacgttatt
    41581 atgttaaggt aacagacgtg tcgtacgtat tgggttccct caccccaatg gttaatcaaa
    41641 aaggtacaat atgaacgttt tctcgcaaac tcaacgctat aaggcgttgt tctggttatc
    41701 gttatttcat cttctggtga tcacctccag taactatctg gttcagcttc ccgtctccat
    41761 ttttggtttc cataccacct ggggcgcgtt tagctttccg tttatttttc ttgctaccga
    41821 cctgaccgtg cgtatttttg gcgcaccgct ggcccgacgc attatcttcg cggtaatgat
    41881 ccctgcgtta ttaatctcct acgtcatctc gtcgctattc tatatgggtt cctggcaggg
    41941 attcggcgca ctcgcccact tcaacctgtt tgtcgcccgt atcgccaccg ccagtttcat
    42001 ggcctacgcg ctggggcaaa tcctcgacgt gcacgttttt aaccgcctgc gtcagagtcg
    42061 ccgctggtgg ctggcaccga cagcgtccac actgttcggt aacgtcagcg acacgctggc
    42121 ctttttcttc attgccttct ggcgtagccc ggatgccttt atggctgaac actggatgga
    42181 aatcgcgctg gtcgattact gtttcaaagt gttaatcagt atcgttttct tcctgccaat
    42241 gtatggcgta ttactcaata tgctgttgaa aagactggca gataaatccg aaatcaacgc
    42301 tttgcaggcg agttaaaggt tcgttatcag agttgtgata agatggatga atgagccgtt
    42361 atggccgttt atcgaaagga agaagtcaat gcgcaatctg gttaaatatg tcggaattgg
    42421 cctgctggtt atggggcttg cggcctgtga tgataaagac actaacgcta cggcgcaggg
    42481 ttcggttgcg gaaagtaacg ctaccgggaa tcccgtcaac ctgcttgatg gcaagttaag
    42541 tttctcgctg ccagcggata tgaccgacca gagcggtaag ctgggaacgc aggccaataa
    42601 catgcatgtc tggtccgacg ccaccgggca gaaagcagtc atcgtcatca tgggcgatga
    42661 tccgaaagaa gatctggcgg tgttggcgaa gcgtctggaa gatcagcaac gtagccgcga
    42721 tccgcagctg caagtggtaa ccaataaagc cattgagctg aaaggtcaca aaatgcagca
    42781 gttagacagt attatctccg cgaaaggcca gacggcgtac tcttccgtta ttctgggtaa
    42841 cgtgggtaat caactgctga ccatgcaaat tacgctgcca gctgacgatc agcaaaaagc
    42901 gcaaaccacc gcagaaaaca tcattaatac gctggttatt cagtaagttt taagatgatg
    42961 aggcggcctc agggacgtgt tccggaggcc gtttttttaa tcgccacgtc agtaataacg
    43021 cgattgcgac cagtcccgcc gccgccagat aaatcaccgg tacgcccgcc cagctcatca
    43081 ccagcccagc cagtggtcca gtcacgccaa gcgataaatc cataaatacg gtgtaagttg
    43141 ccagcgccgc cccctgattt tgctgcggaa ccgcttttac cgccactaca cccaatgccg
    43201 ggaacaccag cgaaaacccg gcccccgcca gtaagacgcc gattttcgcc atccacggca
    43261 tagtcgccac gccaaccagt agcaggccga ttatctcaac gctaaagcaa atcatcgcta
    43321 cgtttaagcc accgatacgg ttaatgccgt tagggaataa caaacgcgta ccgacaaacg
    43381 cacagctaaa cagcgtcagc gcgaaagccg caccgtccca acctttagcg tcataaaaca
    43441 gcgtgataaa ggtggcgatg acgccaaatc cggcggaagc cagtgccagc gccataccgt
    43501 acagccagac gcgcccaagc accgcgcgaa acggcagcgg tttgccttta ctggctttta
    43561 ccgtcggacg cgggatcgcc aacaaaatgg ccaccagcgc cacgcccata atgattaacg
    43621 ctaacgcctg caagccgccc cagtgataaa acacgacgcc taacggcgca cccatcgcca
    43681 tcgccccgta agtgacaatg ccgttccacg aaatcacccg cccgatatgc agcgagccaa
    43741 ccactccaac gccccatagg gtcgatcccg ttccggcaaa actttgccca atcccaagga
    43801 tgacgcgccc caggcaaagt aataacaggc tgatgacagg cagactggcg gttaatcctg
    43861 ccgtcagata ccccagaccg ctcaaaaagc agccgcataa accgaagacg acaatctttt
    43921 tgggtcccag caaatcggcg taacgtccgg catgagggcg gctcagcaag gtggcgaaat
    43981 attgcaggct gataaccaat cctgcccaga aggcgctaaa gcccatcaca tcatggacat
    44041 agcccggtaa tacagcgagc ggcaacccga tggtgaggta gctggcgaag ttaaacatga
    44101 caatggagac aatgcgcaaa ttcaggcgca atccgtttag cgcgggttcg gctacgggtt
    44161 cgggcatgag gatcaccaca tttttacaac agtgtttcat ttttaccatg tgctgacgtg
    44221 aaaatcagca gtaagaatca gaatattgct ggcgtgactc ccgcgctaca cttaacacaa
    44281 aaagtcacaa ggaagcccca atggaaaccc ctcaacccga taaaacgggc atgcacattc
    44341 tgctcaagtt ggcctcgctg gtagtgatcc tcgcgggcat tcacgcagcg gcagatatca
    44401 ttgtgcagct gttactggcg ctgttttttg ccatcgtcct caacccgctc gttacctggt
    44461 ttattcgtcg gggagtacaa cgccccgttg ccattacgat tgtagtggtg gtgatgctga
    44521 tcgcactaac cgcgctggtc ggcgtactgg cggcatcgtt taacgaattt atctctatgc
    44581 tgccgaagtt taataaggag ctgacgcgca aactttttaa attgcaggag atgttgcctt
    44641 ttcttaattt gcatatgtcg ccggagcgaa tgctgcagcg gatggactcg gaaaaagtgg
    44701 ttaccttcac cacagcgcta atgaccgggc tttccggggc aatggcgagc gtgcttttgc
    44761 tggtgatgac cgtagttttt atgctgtttg aagtgcgcca cgtcccttac aaaatgcgtt
    44821 ttgcgctgaa taacccacag attcacatcg caggattaca ccgcgcactt aaaggcgttt
    44881 cgcattatct ggcgttaaag acgctactca gtttatggac aggtgtcatc gtctggctgg
    44941 ggctggcgct aatgggcgta cagtttgcgc tgatgtgggc agtactggcg tttttgctca
    45001 actacgtgcc caatatcggc gcggtaattt ccgccgtacc gccaatgatt caggtgctgc
    45061 tgtttaatgg tgtttacgaa tgtattctgg tcggcgcatt gtttttagtg gtccatatgg
    45121 tcatcggcaa tattttagaa ccacggatga tgggccatcg cctggggatg tccaccatgg
    45181 tggtatttct ttcattgtta atctggggat ggctgctcgg cccggtaggg atgctacttt
    45241 cggtgccatt aaccagcgtg tgtaaaatct ggatggaaac gacgaaaggt ggcagcaaac
    45301 tggcgatttt actggggccg ggcagaccga aaagtcggtt accgggatga ggcgacaagt
    45361 gatacgatac gcactttcat tttccattaa acgttggccc tgatatgtat cggatagttc
    45421 tggggaaagt ttcgacctta agcgcagctc cactgccacc gggtttacgc gagcaagcac
    45481 cgcaaggtcc acgacgcgaa cgctggctgg cggggcgtac attgctttcg cacacgcttt
    45541 ccccgctacc ggagatcatc tatggcgaac aaggcaaacc tgcattcgcg ccggaaacgc
    45601 cgctatggtt caacttaagc catagcggtg acgatatcgc tctgctgttg agcgatgaag
    45661 gcgaagtcgg ctgcgatatc gaagtgattc gcccgcgcgt caactggcgc tggctggcga
    45721 acgccgtgtt cagcctcggg gaacacgctg aaatggacgc cgtgcatcct gatcagcaac
    45781 tggaaatgtt ctggcgcatc tggacgcgca aagaagccat cgttaaacag cgtggcggca
    45841 gcgcctggca aatcgtcagc gtagatagca cctatcactc ctcgctgtca gtcagccatt
    45901 gtcagctaga aaatttaagc cttgcgatct gcacccccac cccctttacg ctcaccgccg
    45961 acagtgtgca atggatcgat tcagttaact gatccgccca cccgactgcc catctattga
    46021 tccagaacag gtaatcagta tgacgaatac ttaaaatcgt catacttatt tccgccatct
    46081 attttaatcc attggggtta ccatgctctc cacactccgc cgcactctat ttgcgctgct
    46141 ggcttgtgcg tcttttatcg tccatgccgc tgcaccagat gaaatcacca ccgcctggcc
    46201 ggtgaatgtc gggccactaa acccgcacct ttacacgcct aaccagatgt tcgcccagag
    46261 catggtttat gaaccattgg tgaaatatca ggcagacggt tcggtgatcc cgtggctggc
    46321 aaaaagctgg actcattcag aagatggtaa aacctggacc ttcaccctgc gtgatgacgt
    46381 gaaattctcc aacggtgaac cgttcgatgc cgaggcggcg gcagaaaact tccgcgcagt
    46441 gctcgataac cgtcaacgtc acgcctggct ggagctggca aaccagattg ttgatgttaa
    46501 agcactcaat aaaacagagc tgcaaattac cctgaaaagc gcctactatc ctttcctgca
    46561 agaactggcc ctgccccgtc ctttccgttt tatcgctccc tcgcagttta aaaaccatga
    46621 aaccatgaac ggaattaaag cgccgattgg caccggaccg tggattttgc aggaatcgaa
    46681 actaaatcag tacgatgtct tcgtccgtaa cgaaaactac tggggcgaaa agccagcgat
    46741 taaaaagatc acctttaacg tcatcccgga cccgactacc cgcgcggtgg cgtttgaaac
    46801 tggcgatatc gacctgctgt acggaaacga agggttatta ccgctcgata ccttcgcccg
    46861 ctttagccag aatccggctt accacaccca actgtcacag ccgatcgaaa ccgtgatgct
    46921 ggcgctcaat accgccaaag cccccaccaa cgagctggca gtacgtgaag ctcttaatta
    46981 cgcggtaaac aaaaaatcgc tgattgataa cgcgttatat ggcacccagc aggtcgccga
    47041 caccctgttt gccccttctg tgccttacgc taatctcggc ctgaaaccgc gccagtacga
    47101 tccgcagaaa gcgaaagagt tgctggaaaa agccggttgg acgctgcctg cgggcaaaga
    47161 catccgcgag aaaaatggtc agccgctgcg cattgaactg tcgttcatcg gcaccgatgc
    47221 gttaagcaaa tcgatggcgg aaatcattca ggctgatatg cgccagattg gcgcagatgt
    47281 ctcgctgatt ggcgaagaag agagcagtat ctatgctcgt cagcgcgacg gtcgttttgg
    47341 catgattttc caccgcacct ggggcgcgcc atacgatcca cacgccttcc tgagttcaat
    47401 gcgcgtaccg tcgcacgctg acttccaggc acagcaagga ttagccgaca agccgctgat
    47461 tgataaagag atcggcgaag tgctggcgac ccatgacgaa acgcaacgtc aggcgctgta
    47521 tcgcgacatt ctgacccgtc tgcatgacga ggcggtttat ctgcctatca gctacatctc
    47581 gatgatggtg gtatcaaaac cggagctggg taatatcccc tacgcgccga tcgccaccga
    47641 aattccgttc gaacagatta aaccggtgaa accttaatgt tgcgttacgt attacgccgc
    47701 tttctgctgc tgatcccgat ggtgctcgcc gcctcggtga tcatttttct gatgctgcgc
    47761 ctcggtaccg gcgacccggc gctcgattat ctgcgtttat ctaacctgcc gccgacgccg
    47821 gagatgctgg cctctacccg caccatgctc gggctggatc agccgctgta cgtccagtac
    47881 ggtacctggt tgtggaaggc gctgcatctt gactttggta tctcattcgc cagccaacgc
    47941 ccggtactgg acgatatgct gaacttcctg cccgccacgc tggaacttgc aggtgcggcg
    48001 ctggtattaa ttctgctcac ttccgtaccg ctcggtatct gggcggcgcg ccaccgcgat
    48061 cgtttgccgg atttcgccgt gcgctttatc gcgtttcttg gcgtgtcgat gcccaacttc
    48121 tggctggcat ttttgctagt gatggcgttt tcggtgtatc tgcaatggct acccgcgatg
    48181 ggctacggca gctggcagca catcatttta cctgcggttt ccatcgcctt tatgtcgctg
    48241 gcgattaacg cgcgactgct gcgcgccagt atgctggacg tcgccggtca gcgtcacgtc
    48301 acctgggcgc ggctacgcgg cctgaatgac aaacagaccg aacgccgcca tattctgcgc
    48361 aatgcctcgc tgccgatgat caccgccgtg gggatgcaca ttggcgaatt gattggcggg
    48421 acgatgatta tcgaaaacat ctttgcctgg cctggcgtcg ggcgctatgc ggtgtcggcg
    48481 atttttaacc gtgactatcc ggtgattcag tgctttacgc tgatgatggt ggtggttttt
    48541 gtggtctgta atttgattgt cgatttgctc aacgccgcgc tggacccgcg cattcgtcgt
    48601 catgaaggag cgcacgcgtg aactttttcc tctcttcccg ctggtcggta cgcctggcgc
    48661 tgatcattat cgccctgctg gcgctgattg cgctcaccag ccagtggtgg cagccgtacg
    48721 atccacaggc aattgatttg ccgtcgtgcc tgctttcgcc ggatgcgcag cactggctgg
    48781 gcaccgatca cttaggtcgc gatattttct cgcggctgat ggcagcgacc cgcgtgtcgc
    48841 tcggttcggt aatggcctgc ctgctgctgg tgctgacatt agggctggtt attggcggca
    48901 gcgccgggtt gattggcggg cacgttgatc aggccaccat gcgcgtcgcc gatatgttta
    48961 tgaccttccc gacctcgatt ctgtcgttct ttatggttgg cgtgctcggc accgggctga
    49021 ccaacgtaat tatcgccatc gccctgtcgc actgggcgtg gtatgcacgc atggtgcgca
    49081 gcttggtgat ttcactacgc caacgcgagt ttgtgctggc gtcacggctt tccggtgcgg
    49141 gccatgtgcg ggtgtttgtc gatcatctgg caggcgcggt gatcccttcg ctgctggtgc
    49201 tggcaacgct ggatatcggc catatgatgc tgcacgtcgc ggggatgtct ttccttggcc
    49261 tcggtgtgac cgcgccgacc gccgaatggg gcgtgatgat taacgacgcg cgccagtata
    49321 tctggaccca gccgctgcaa atgttctggc cggggctggc gctgtttatc agcgtgatgg
    49381 cctttaacct ggtgggtgac gcactgcgcg atcatctgga tcctcatctg gtgacggagc
    49441 acgcacacta atgccgcaac agattgaact acgtaatatc acgctacagg ccgcgcagcc
    49501 gctggtacac ggtgtatcgt taaccctgca acgcgggcgc gtgctggcgt tagtcggcgg
    49561 tagcggcagc gggaaatcat taacctgcgc cgcgacgctg ggcattttgc ccgctggcgt
    49621 tcgccagacg gcgggggaaa ttttagccga tggcaaaccg gtttcgcctt acgccctgcg
    49681 cggcatcaaa attgccacca tcatgcagaa cccgcgcagc gcctttaatc cactgcacac
    49741 catgcacacc cacgcgcggg aaacctgcct ggcgttaggg aaacccgccg atgacgctac
    49801 gcttaccgct gccatagaag cggtggggct ggaaaacgcc gcgcgcgtgc tgaagctgta
    49861 cccgttcgag atgagcggcg gcatgttgca gcgcatgatg attgcgatgg cggtgctgtg
    49921 tgaatcaccg tttatcatcg ccgatgaacc gaccaccgac ctcgacgtgg tagcacaggc
    49981 gcgcatcctc gatctgctgg aaagcattat gcaaaaacaa gcgccgggaa tgctgctggt
    50041 cacccatgat atgggcgttg tggcgcgtct ggcggatgac gtggcggtga tgtcacaagg
    50101 taaaattgtc gaacagggcg atgtagaaac gctgtttaac gcccccaaac atacggtgac
    50161 gcgcagcctg gtttccgctc atctcgccct ctacggtatg gagctggcat catgacctta
    50221 cttaacgtct gcggcctttc ccatcactat gcccacggtg gatttagcgg aaaacatcaa
    50281 catcaggcgg tgctgaataa cgtttccctg accctgaaaa gcggcgaaac cgtcgccctg
    50341 ctgggacgca gcggctgcgg aaaaagcacc ctcgcgcggc tgctggtagg cttagagtct
    50401 ccctcacaag ggaatattag ctggcacggc gaatcgctgg cgaaacttaa tcgcgcccaa
    50461 cgtaaagcct tccgccgtga tattcagatg gtgtttcagg actccatcag tgccgtgaat
    50521 ccgcgcaaaa ccgtgcgcga gatcctgcgt gaaccgatgc gccacctgct atcactgaaa
    50581 aaatccgaac aactggcgcg cgccagcgaa atgctgaagg cggtcgatct cgatgacagc
    50641 gttctcgaca aacgcccacc gcagttaagc ggcggccagc tccagctcgt ctgcctggca
    50701 cgcgcgctgg cggtcgaacc gaaattgctg attctggatg aagccgtttc taaccttgat
    50761 ctcgtgttac aggcgggcgt cattcgcctg ctgaaaaagc tacaacaaca gtttggcact
    50821 gcctgcctgt tcatcactca tgacttacgc ctggtggaac gcttttgcca gcgagtgatg
    50881 gtgatggaca acggacaaat cgccgaaacc caggcggtgg gagataaatt aaccttttcc
    50941 tctgacgccg gacgtgtgct acaaaacgcg gtattacccg cattccccgt gcgccgtcgc
    51001 acctcagaaa aggtttaacg ccaatgcaac gagtcaccat cacgcttgat gacgatttac
    51061 tggagacgct ggacagcctg agccagcgtc gtggttataa caaccgttcc gaagctatcc
    51121 gcgatattct gcgtagtgcc ctggcgcagg aagctaccca gcagcacggc acacaaggtt
    51181 tcgcggtgct gtcgtatgtg tatgaacacg aaaaacgcga cttagccagc cgtattgttt
    51241 ccacccagca tcatcaccac gacctctccg tcgccacgct acatgtgcac atcaaccacg
    51301 acgactgtct ggaaatcgcc gtgttgaaag gtgacatggg tgacgtgcag cattttgccg
    51361 atgacgttat cgcccagcgc ggcgtgcggc acgggcattt gcagtgcctg ccgaaggaag
    51421 attgagttat ttctaaaaca aggaaagaaa agatattaac tgaacttaat ttataaaatt
    51481 aagttcagtt gcatagatag atcctgtaaa ggcagatatt ctgcgtaatg tacgtcgcta
    51541 aaatttataa atgattcaaa cgctcattca gtgtttcaat aatataggta ttaacagaga
    51601 cttgttgctg cgcagccgca ttattaaggc gttcactcag cgattctgga tagcgtaacg
    51661 taaaggtctt aatcttttct gtacgcgcat acggctcaat acccgcagct ttacaatcct
    51721 caagatactc tcgcagggaa agctctcctt ctttttgcag cccctgaata ctgtcagaga
    51781 caaaatcaca ataacctgac aagcccagga atttgccacg aaatgcattc agttctggca
    51841 cgtaggtaat aacagctggt tgcccggcaa tctccataga gtttggcgtc ttcagtttga
    51901 tcatggtttt acccctatac ttagtaacca gtcacacacg ctttctacag cgcctttatc
    51961 ggtatctggc gacggatgag gccgatggaa acacgcaacg ctcatattta gtatgaactt
    52021 gcaacgcgaa cctcttcctt ccttaatttc tccgcctaat gctttaacca gtgactctat
    52081 atcagaccac ttgatccctt gaggaacagg agttttaaat atttgatcca atgtattttt
    52141 ttgtttcttc cgtaaagcct gtaccttggt tctcatggca tgtccttatg acttcacttt
    52201 atgacatcac agtatgaagt caagtttccc ttccttacgc cattgtccca atcgtcttcc
    52261 tgaatcgcag cagtgcgatg gtgaaaaacg cgccgccaat tgccatcagc gtcaggaact
    52321 gcggccagac gatttcgaat ccggcacccc ggtagaggat ggcctgcgcg aggctaacaa
    52381 agtgtgtcgt cggcatggtc agcataatgt cctgcaccat ctgcggcata ctttcgcgcg
    52441 gcgtggatcc accggaaagc atttgcagcg gcagcagcac cagaatcacc agcagcccca
    52501 gttgcggcat tgaacgcgct atcgtcccca taaaaatgcc gattgacgtg gtggcaaaca
    52561 gactgagcgc cacgcccagc ataaacagcg ggatcgagcc ttcaatcggt acgcccagta
    52621 cacctttcac catcagcacc agcgataatc ccgataccac cagcaccacc agccccatcg
    52681 accagatctt cgccatcatg atctcaaacg gcgttatcgg catcaccagt aaatgttcca
    52741 ccgtgccgtg ttcacgctcg cggatcagcg ccgatccggt caatacaatc gccagcatgg
    52801 taatgttgtt gatgatcgcc atcaccccgc caaaccacgc gggatcaagg ttcgggttaa
    52861 agcgcatccg ggtttccagc gataccaacg gttcgctgtt atcacggtag cgcgcgacaa
    52921 agctgttcac ttcaccgttg ataatattct ggatataccc attgccggta aatgcctggc
    52981 tcatgcgcgt ggcatcgacg ttcacctgaa tatccggctg gcgtccggcg aggacatcac
    53041 gctgaaaatt aggcggaata tttatcgcga aggtatagcg tccggcgtcc agtccggcat
    53101 ccatctcatc ggcggtgatc atctccggtg gcaaaaacca cggacgatag aagctgttaa
    53161 cgatccggtt cgataactgc gattgatcca tatcggcaat ggcgatcggc gcgaggttca
    53221 acgatcctgg cgtaacggtc gctgacgaat acaccgacac cgtaaacgag aagacaatca
    53281 gcgtcagcat cgctttatca ccgagcagac tgcgcaactc tttgataccc agattaaaaa
    53341 tattgcgtaa atggcgcatc atccctcctg ttttttcagc agcaggatgc ttaagcccat
    53401 caccagcggg atggctatca gcaacgggat aaaaagttgc cacaaatcag tcagatccag
    53461 cgctttcgag aacgttccgc gggcgatagt cagaaaatga ctggtcgggt aaacctcgcc
    53521 gatccaacgt ccaggccctt ccagcgaagc taccggatcg atcatcccgg aaaactgtgt
    53581 cgccgggatc aatgtgataa tcgccgttcc gaaaatggcg gcaatctggc ttttcataaa
    53641 ggtggagatc agcagcccca ttccagtggc aatgatgata tacagcagcg ccgccaaggt
    53701 gagcgtcagg aaactgcctt tatgcggtac gccaaacaca aacaccgaca ggccgcagag
    53761 caggaaaaag ttcagcatcc ccagcgcgat gtacggcagc tgtttgccaa gcaaaaattc
    53821 gctacgggtg gtgggggtca cgtaaaggtt gataatcgac cctagctctt tttcccgcac
    53881 cacgctaagg gcgcttagca ttgacgggat catcatcagc agaagcggga tcaccgccgg
    53941 aacaatcgct ggcaggcttt ttacgtccgg gttatagcga taacgcgtct caatgttcat
    54001 cagcccgctt tggctggcgg gtgtcgattg tcggctcgcc acatcctgaa gccaactctg
    54061 gtgcattgcc tgcacgtaac cttttaccgt ttcggcacga ctgggcatcg ctccgtcgat
    54121 ccagacgccg agttccacag gcgtaccacg cgcgatatcg cgcccgaaat tgggcgggat
    54181 ctcaatggcc accgtgatat cgcccgcacg catccgacga tcaagctcgt cataactggt
    54241 gagcggcggc tgttcgataa agtaacggga accggagagg ttgagtgtcc acgcctggct
    54301 actgacggtc tggtcgcggt cgagcaccgc aaagcgcagg ttttccacat ccatactgat
    54361 gccgtaaccc attatcagca tcaggatcac cgttcccatc agcgccagcg tcgaacgtac
    54421 tggatcgcgt cgcagttcca gcgcttcgcg gcggctgtag ctaaacagac ggcgcaggct
    54481 aaatccctga cgcggcgcgt gggtggtgtc gtgtaccacg ggcggcgctt cggcttcgtt
    54541 gctctgccct gccgcttcct gcaaataggc gataaatgcc tcttccagac tggcggctcc
    54601 gcgtttctca accagttcct gcggtgtacc gctggcaagc acttttccgg cgtgcatcag
    54661 tgagatgcgg tcgcaacgtt ccgcttcgtt cataaagtgg gtggagatga agatagtcac
    54721 tttgtcctgg cgcgagagat cgaccatcaa ctgccagaac atatccctcg ccaccggatc
    54781 gacaccagaa gtaggctcat cgaggattaa catctccggg cgatgaatca ccgccaccgc
    54841 cagcgaaagc cgctggcgaa tgccgagcgg caatgactcc ggcagaacat cttcaacgtc
    54901 gttgagctta aaacgctcgc tcatttcagc cactcgcgcg ggaatttccg cttccgggat
    54961 gtgaaacaaa cgggcatgta actccaggtt ttgccgcacg gtgagttcgt tatagagcga
    55021 aaacgcctgc gacatatagc ccacacgacg gcgggtatcg atatcttttg gatcaaccgg
    55081 ttgcccaaat agccacgcct caccttcgct ggcgggcagc agtccggtaa gcattttcat
    55141 ggtggtggat ttaccgcagc cgttcgaacc aaggaatcca aaaatctccc cgcgtggaat
    55201 gcggaaattg acgtgatcaa cggcaacgaa ggaaccaaaa cgcatggtca gatcgcgcgc
    55261 ttcgatggca atctctgcgt tttcaggttg atacggtggg atcactaccg cctgatgcgc
    55321 ctggcgttgc gcttgcggta acagattaat aaatgcttct tccagcgtgg cgctttgcgt
    55381 ttgctgccgc agctcttcgg cgctgccggt tgccagcact tctccggcat tcatcgctac
    55441 cagccagtcg aagcgttcgg cctcttccat ataggcggtg gcgaccagca cgctcatatt
    55501 gctctgccgc tggcgaatac tgtcgatcag atcccagaac tgggcgcggg agagcgggtc
    55561 aacccccgtt gttggctcat caaggatcaa cagttccggg tcgtggatta acgcgcagca
    55621 cagcccaagt ttttgcttca tcccgccgga gagtttacct gccggacgat cgcgaaacgg
    55681 tgctaacccg gtgctggtca gcagctcatt aattcgcgct tcccgctccg ctttatcgtg
    55741 accgaacagg cgagcgaaaa aatcgacatt ttcatacacc gacaaggtgt ggtagaggtt
    55801 tttgcccagc ccctgcggca tccaggcgat gcgcgggcag acgtctcggc gatgcttcgg
    55861 gtcgtgcata tcgccaccca gcaccaccac attgccctgc tcaatgacgc gggcaccgga
    55921 aatcagtgac aataaactcg acttcccgac gccatccgga ccaatcagcc cgaccatgca
    55981 acgggcctga atatcgagag tgatattgtt cagcgcaacg gtttttccat aatgctggct
    56041 cacgcccgcc agttgcgcga caggcgggac gggaaccagt tccagatgcg tcattgcggc
    56101 aacctcacca cgaggtcgtc aggccacgga agttcttcat tcacccgcac ccacgctacg
    56161 cccggtaaac cggttttgac atattccaga tgctgctgga gtaattccgg tgggatacgc
    56221 gctttgacgc ggaacatcag tttcagccgt tcatcgctgg tttcgacggt ttttggcgtg
    56281 aactgggcga cactggcgac aaaactgatg gttgcaggaa tacgcagatc tggcgcggca
    56341 tcgaggatca gccgggcttc accgcccagt ttcagcgtgc ccgcctgttc ggttggcagg
    56401 aagaaggtca tatagacgtc gctgagatcg accatattca gcacccgacc gcctgccgcc
    56461 agcacttccc ctggctcggc aacccgatac tgcacgcgtc cgtcacgcgg ggctttcagt
    56521 tcactgtcat cgatatctgc ggcaatgcgc cgttcagtgg cttgcgctgc ttcgacccga
    56581 gtttgcgcct gaatgatatt ggtgcgtgct gcttctatag ccgctttaga agccgaaacc
    56641 tgggctttcg ctgattccag cgcagctcgg gcgctctcag cggcggcgcg gtcgtcatcc
    56701 agctgttgcg cagaaatagc ccctcgttgg gcgagcgagc gggaacgcgt atgacgtttg
    56761 gcaacagagt ccagttcggc ctggcgttga ttgaccagcg actgtgcggc acgagtttca
    56821 ctttgtcgtt gctccagcaa agcctgcgcg gcggcaacgg cgctttgtgc ctctttgatt
    56881 tgcgcgatgg cttccagtcg ctgttcctgc aacacgcgag tatccatctt cgccagcact
    56941 tcgccttcgc gaacaaactg gccttctttc accagaatgg tgtcgatacg cccggcaatt
    57001 ttgctggcga tatccacttc cgtcgcttca atgcgcccat tgctgacagc aaagccttcc
    57061 ggcacacccg ccgggcgcaa cagccaccac gccacgacgg cagccaccgc cagtgccccg
    57121 acaacccacc acgccagatg gcgcttactc ttatccataa tcgacccgcc ataatccctg
    57181 taagcaaacg acgcgtgtta caactgccgc cgaacgacgt tcagcggatt cttccatccg
    57241 ggataagaag gaaacgacaa atacgaccag cgcgcagagc attgagcgcc aacggggaag
    57301 gcttagtgaa aacgggtagt aatgttgtca tcatcagcat tcctggccgt aaatgaaaca
    57361 cgcatactca taagtgtgat ctataaaact ggctgacaaa acggtatcca tcctgttgga
    57421 tcgcatttca cgcctgttca gttgcaggaa cagagcattc ctttttacgt tttatcaaca
    57481 gtctcaacca gatagtaaca ttaatattca tgttgtctat ggttcagagc tgtaacattg
    57541 tgcgccttct aatcaaaggc ataagtccat ttctgttttt ttcatgctgg taagacttcc
    57601 agggagtggt tatctctggc cctgcaccag ggttaatttg gcaattatgc atgctgaaca
    57661 ttcttccagc atgtttactg tcacgggcgt taagacctaa aactgtcccc gtttcaccta
    57721 tgcgttctct cccaccattt ccgttatgtg caacaacttg atttatacct aacatttgtg
    57781 aacatgctaa tagtgcttca agtttaggtc tgtaatctgt cgaaacagaa aaataacgat
    57841 taaaggatcg acaagagata gcccggctag ttttaaactt tttgccatta attatagcat
    57901 gatgcttttt attaataatt tccgctaatt cgaccggatc gagagcattg gtcatttcat
    57961 caatagagac agttatcggt cctataatgt aacgttttcg ctgatcatca aaagttatcc
    58021 catgatgatt tgccattatt ttgtatttgg ggtcatagaa acatacagga taatctttta
    58081 tcccgtcgta ggtatcctca tcaagtggtt tcatgcgcgt gaagttttgc atatagttac
    58141 cattgcaatt cgtttcatga tttccagcta atacatgaac attttcattg atactcatta
    58201 tgcgttttaa caaatcgatg ataaattgat cgccacagat attactaaat cgatcgccgg
    58261 tttggtcgcc aagaaacgta catggcgtga ctgcgtcttt attaataacc aaataaggct
    58321 gaagactttc taaaacatta tctcttctgt tcagtatact gattattgta ttaacattag
    58381 tgtcaagttg ctcattaaag ttctccctta gcaatacgtt gagaacttca tcttctatat
    58441 taagcaactg gctaaggaaa ttttctcctt cagccatgat tcccaaatgt cctgttgcaa
    58501 aaagcgcgta caaaactgca ccgactgagc catcggtatc gccaaaatag gtcggcctgg
    58561 tacttgtggg atcaacaggt gaacaattgg agaaataggt tctgtcagcg aactggggtt
    58621 ttataattgt ttcgaattta atatcaaata cattgtattg cgatatttcc cagttccaga
    58681 tgggtttgta cgactcagta actgcaacat cattagcatt atcatttact gtcatattat
    58741 taagtaaata aacaatgctg caccaattaa tatcggtatt atcaagaggg caaccgtaat
    58801 ttttactatt aattgtcaca ataaactgat aacagttatt cctacgtaga atatgagcgc
    58861 gcatgcccga tagcatacta tatttttctg aatgattcgt ttgtatggta aatgaaattt
    58921 tgtcatcttg aatagattgt tggaaacgtt ctatttgcca atcatcaatt ttttccacaa
    58981 cttcaccgag cattgtcctg gcaatacttg atatatcatc acctatacca tttgcaatat
    59041 acccaaacag ttctttagtg aagttggtaa catgctcttg caccatatca tttgttgcga
    59101 ttgtcgttgt actgccatta gtgcctgcca cagttccaat tttcatcttt tacctcatat
    59161 tattcaccgc gaggaataca tagttaacat tttatcttat gacatacacc tcatattcga
    59221 tggggaaata attatatttt gcacaaatca ttcagcctta aagcataata tgaaatttct
    59281 tatctcatag cagagaaagt ccctaaatat tgtagaggcg tcatcagtac atacctctac
    59341 aatcgattaa gaaaacctat gaaatactgg caaaattaaa aagagatacc acagcactca
    59401 tgaagtttta ttgaggttga aaacaactaa gccctgggac acctttttta tcaccacgtt
    59461 cgttaggtgc tatcttccaa gaatttagcc ccatggtata attatcaatt ttttgatgct
    59521 ggtctacccc taatccataa tggccaaatt tctgtttagt ttttttgagc gtctgtccac
    59581 atttgaagat atcttcaggt tttggtctga aagcagtagt gttattaaaa taattttcat
    59641 atacaggtgt gggtgctata gcattgcttc taattagatg gagaagatga tcatccatgt
    59701 gttgtttatg ttttttatta catatatttg ctaattcctc aggatcaatt gggtctttca
    59761 tctgattaaa tggaacctgc aaagcaccga gacaataaca ttgattttct tcatttctta
    59821 ttattccgtg atgactggtt aaaactttag tttctgagtt ataattacaa acatcgagcg
    59881 ttttaattaa gttatacgta tcaccggcag agagtttata attttctagt cgtgcagtat
    59941 agttaccatt aaaattgatt tcatgatttc cagccagaac gatgacattt ttattaatac
    60001 ggctatcttt attaccctcc atatttcgca tagaatttag caaagtaagg atatatttat
    60061 caccaaaaat agtactaaat ctatcgcctg tatgatcccc aaggaagata caaggggtta
    60121 tcgcatcgtc ctttaaaaaa acatgttgat gcaactcatt caatatcatg tctctcgtat
    60181 ttaataaatt gtagagcaat ttattatttt tatgtatgta tgcagatgaa gccatgtctt
    60241 cgtgttttaa taattcacat aataaactcc aacccttttc cctgatgcct atatgccctg
    60301 aaacgagcaa tgaataaagt acagcgccta ctcttccatc tgtatcgcca aaataagccg
    60361 tgttggcatt ttgtgggtta atcacaccac attgtttata aaagtctatt ttagaaaaca
    60421 cagatttaac atagctatca aacttatcct taggaaacgt ctctttagag acatcccaac
    60481 gccagtcagc tttaaaatta ccgctaagaa gagtaaagaa attttcaggt atgatagaaa
    60541 gcccattaac tttataaatt atgtctgatt tatctccgga gaaattaata aaataataac
    60601 catccttaca ctcaatctta actgtaatgg gagacgataa ttttatcaca tggttattgt
    60661 ctggtataat tgtaaagata aacgtgttag tagtattatc ggcaaaacta ttatattctt
    60721 gaatatctat agatggctca ctattgtaaa accatatttt ctccatctga agactatgtt
    60781 catttaccct ccagtgacca tgttgcagga aatagatgat atgcatgcca aaatcgttat
    60841 ctatttgcga atcattatcc agcttatcaa tttcaactat ctttgaacga tgtgttttat
    60901 caaccttgct atggtgttga tgattattaa ctttatctga atgcttatct ttatggtttg
    60961 aagggatgta cttaaatata ttgtcaattg ctttcataaa taacccgcga tatttttgac
    61021 ctgttagtaa aaaataaatg atacatgtct gaaagacata tttacgacga tataaaacaa
    61081 taatacttat atgttcgctg attttaagca ccgagactat ccggtctttt actaaataat
    61141 ggaaaacaat ccatccctgg aagacaattg taaaaaaaaa caacagaata agaattaatc
    61201 ttaggataaa tatttattta tcatggcctt ttgaccgcta aaaaatggca ctcggcacag
    61261 taaaccccgc tatagttatg ctatgcattt gtctttaatg atatctcgtc gttatattaa
    61321 caggctgaaa ttatcattaa tgcacatttc aatattagca gggatacctg aggagtttaa
    61381 agtgaacata tatatcgggt agcttttcaa attaatccct ttgattatag gcttaatttg
    61441 catcgcgtta ggtggctttg tgctggaaag ttcagggcaa cgtcacgggt catgtgctga
    61501 tttctctggc ggccatatgc ctggcattat tcactaccgc atttattatc atttcgcaac
    61561 tcacgcgcgg cgttaatacg ttttacaata cattgttccc cattattggc tatgcggggt
    61621 caattatcac catgatatgg ggttgggcac tgttagcagg taatgatgtg atggcagacg
    61681 agtttgttgc cggccatgtt atttttggcg ttggtatgat tgccgcatgt gtatcgacgg
    61741 tggcagcgtc gtccggtcac tttctgctca ttcccaaaaa tgcagcgggg agcaagagcg
    61801 acggaacacc ggtacaggct tattcttcat taatcggtaa ttgcctcatt gccgttcccg
    61861 ttttactcac cctgctcggt ttcatttggt ctattacgct gttacatagt gccgacataa
    61921 ctccgcatta tgtcgccttg ggttaaccgc aatctgtgcc tgtctaattg gcctagtggc
    61981 cacaatcgtc catcaaacac gtaatacgtt ctcatccaaa gagcactggc tgtggtgtta
    62041 ttgggttatt tttctcggct cagtcacggt gctgcagggg atatacgtct tagtcagttc
    62101 cgatgcaagc gcccgactgg ctcccggcat tattctcatt tgcctcggaa tgatctgtta
    62161 cagcatattc tcaaaagtct ggctactggc actggtctgg agacgtacct gttcgttagc
    62221 caacagaata ccgatgatac cggtatttac ctgtctgttt tgcttgtttt tgtcatcatt
    62281 cctcgcggaa atcgcacaaa ctgagctgga ctactttatt ccatctcggg ttctggttgg
    62341 attaggcgca gtatgtttta cattgttctc tattgtttca atattagagg ccggttctgc
    62401 gaaaaaaaat taaaatttac ataatcccag cgttattcat atcttaacgc tggtttttta
    62461 cgaacttagt gaaataatgt agaatataaa attataattt tatattggag cacatgtgtt
    62521 aatgatcaac cccgggatac taaaaatata gacattatta aacttgctta gcctcgcatt
    62581 gcctttacct gttaaagatg catcgagaaa attgagtctg aagttctcat cctttatctg
    62641 attttccagt cccgaaactt tggccttagc attacctgag atccgtccgt tactatctat
    62701 tttgtaggta aaaaaatacg aatgggaaaa tttcctcggt gtatcttcat cagttaagcc
    62761 aacaatcgta aaactaccgc taccatcttt ttttaaaatg actgaaaggt tagcagagta
    62821 gtataaagaa tgatctgttt tagcatttct tgttaacgtc gacgtacatt caagagacgt
    62881 tgtgctacca tcacgagcct tccagagagc aaaaaaacac acctgtagca atgtgaaaac
    62941 aagtattaat ataaaataca ttttcttatt agtcatataa tagcacctct gaacaaattg
    63001 cataagtttc gtcattagag gattttatac atcttgataa ataccgctgc ccttccccaa
    63061 gcaaataatg catactattt acatcatata taaaatttgc acctggcaca cagggtaaat
    63121 gctttttcga aatttcagcg gcctctattt gagatttagc gatgccaatc tcgttaagat
    63181 tctctgaaag atataaatca caggtgtcaa tcttacctga tttaaccaat ggataatccg
    63241 ttatactgta ggtatcttta aaagccacgg caaacatttc aataataaca attactacca
    63301 caagcgaaat caaaatgcga aaaagaggaa tactcctttt gcgctctact ttttcaacaa
    63361 catcttcttg taaatgagaa tcatcataag attgtttact tattgttatg ttttcactga
    63421 tgtaaaagcc tactttgggt atcgtaagaa tcacattttc ttcaacacca atatttttaa
    63481 aacttttgcg taacagggat atatattggt tcaatgtgtt gtttgacaga tcaaatgaaa
    63541 atctctccaa aacgctatca atgattgagt tcctgtcaac cgctccacca ttactttcca
    63601 gtaacactgc caaaatacac cccgccgtag ctggaaaatt tatctcatcc tgggtattta
    63661 cattcgagag cgagcatttg ttcggatcaa aaataatcgc gtcattgata atgaaaaaac
    63721 atctccgctc catgcaggca acaacacaaa ataagatttt gtcattttag cctattcctg
    63781 gtggaactga cacaagaaac taaaatacaa atataaccct gaaaaagtca tataaaactt
    63841 atatccacag aataaaaaat caaacatgtc agttttatca atataaagta tcgcaaggcg
    63901 aaaagtgatg caagaatact cctaagtaaa tgaaagcgca ccacatgaaa ttatcaacac
    63961 atgtaatttc atcattaggt gctatcgaaa ataactcagg agctacttaa gatgaataat
    64021 gtcttactca tgagacccga tgactcagaa tttatgatac ctgatgactg gtcagggcta
    64081 aacgcaaaag gcattacata cgaacaagat aatagagtga cagagtcttt tctgcctttt
    64141 aaatcttttt attgttcacc ggtgtgcacg acagttgaac tcatctcata aaataattgt
    64201 tttgctatgg gtatccgcta ccttctggaa aagaaaagag acttaaggct aaatcacttt
    64261 aaagccttct ctcataaatg cgagacaaaa cctgacgtgt taattatgac cgggtgcacc
    64321 gtcgctgata cttatagtat attctcacgc attgttgaag aaaatataat gaaaacgatg
    64381 acacctatcg tcatcattac tgggcatcac tcagaagatc tttgttctct ggcatcgaaa
    64441 ctgggaggag ttaaaataat atcaccgaca gaaaagattg caaaaataga aaacgctatc
    64501 aatgatgctt ttaacagcaa gcctaaaaaa ataagatttt taacagatat actctctcct
    64561 caacaaattg agaccatgaa aatggttgcg cagggtagat ccgttaatga gatttcagcc
    64621 gcaatgaata ttagcatcaa gaccgtgtat gcacataaag ttcgtgctat caataagctg
    64681 ggaattaaga aaaaagttta tgaagcgctt ttttataaag cgctaattca gttcaactga
    64741 tgtaaaaatc accctcgctt aataaataat atattctatg tcttttttta taagttaaaa
    64801 aaggcgggat atcaaatccc gccatgaaat taactaatca tctgcattat tctgcgtaga
    64861 tataagcctg ggtacggatg gtgtaagagt ctggctcaac aacctgttct ttcatatggg
    64921 aaacgacgcg ataggcacca cctgttccag tatttttcag catcatataa cgcttaccgc
    64981 caatctcttc ggaggcagct gaaggaggat cctgctcaag cgttgtcagg gaaactttca
    65041 gtttatggtc cgggttattg tcaccagaaa ttactgcatc cgctgaagta ctgctctccg
    65101 cataaatatc atccgggaaa gttacagcat agtcgtggct ctgcccatca gatgcgccaa
    65161 caatacctgt tgccaaaggc gtccatgcag ttaacgtacc gctatatacc cctttcactg
    65221 gttgaatggt attattaaca acgataggct tggtcaaagt aagattagct gacgcttcag
    65281 taccctggct tactaaagtt cccgcctgag aagcaaaagc tgtccccgcc agcatgattg
    65341 ctaacaatgt tttcttcatt atattttctc catgattttc atttacactg caaaatgcag
    65401 tttctttctt tgcatttacg tgctatcgcg aaaatgcttc caccaaataa tggaaacatc
    65461 ttcacaaaaa taaattatga gtttcttaat ttagaaacta taaacatgtc gccgctatct
    65521 ggtataaccc tgatgcactt ttcttcgcag gaagttggta ttttgcgata caggattgat
    65581 ctttagaata accccatttc acattaaact cgccttccgc actcaagcca gaaacataag
    65641 caataccatc ttcacctaca atgccggcca tattttcagt gtctttgcta taactaatca
    65701 cagcgccgaa ggggatgggt ttaccctctt tctgttttaa ggttactaat cctttatccc
    65761 cgctcttcac tgccaatgtc gcaggcacaa cggctccagc tgtcggtaca accaattgac
    65821 tagtttgtgg taagtcgtaa ttcttaccta tcgttttttc atccagaata acatcgtctc
    65881 ttcggtatgg ctgcactggc tgtattgcat acccacgaaa atcagtactc agtcccggcc
    65941 agccattaac gttaaccccc gttgctccgg gtgcctctat aagggctaat gtcccatcca
    66001 cattttgacc aaatgtaagt ccatcccttg taataagtat cccaccgctt aaatttgcac
    66061 ctagctgttt catgttttcg ctggagctgt aatggctact aaactgacca tatgatccat
    66121 ttttgccaac ttgcacaatg ttcatcgtgc tcttcggttg tgactgatga gcgttagtta
    66181 tatttgtcgc tacatcccac catacaccac cactattctg acctgataat gaaacctgat
    66241 agttattacc ctgctctccc gaacgactca tctgtgaaga cgcataaatt tgattttcgt
    66301 cacccagcga taatggcact gaaataccca cgcttaattt ccgatctact ttccatttat
    66361 gcgatgtcag caagtcgttt tttgcccagt taatggagaa tgttatgcct ctccataccg
    66421 tactataact caggctggta gaccgcgtgt ttcctgtgcc ccgggaagaa ttatacctcg
    66481 tggtattgaa gttcaaattc ccgatattac caaggggctg ggataaggat atcgtagagc
    66541 catcgtgact attcttatct ctttttctgt tccgttcatc ctgtgcactt actgcttcag
    66601 tcagtgtggc ataacctgat gcataacggt tcgtggttat agaca
//
LOCUS       DAJTQQ010000009        65979 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000009 DAJTQQ010000000
VERSION     DAJTQQ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.009"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(89..568)
                     /gene="folK"
                     /locus_tag="OO309_000657"
     CDS             complement(89..568)
                     /gene="folK"
                     /locus_tag="OO309_000657"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR5845916.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(565..1983)
                     /gene="pcnB"
                     /locus_tag="OO309_000658"
     CDS             complement(565..1983)
                     /gene="pcnB"
                     /locus_tag="OO309_000658"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR5845917.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2022..2948)
                     /gene="gluQRS"
                     /locus_tag="OO309_000659"
     CDS             complement(2022..2948)
                     /gene="gluQRS"
                     /locus_tag="OO309_000659"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR5845918.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(2985..3440)
                     /gene="dksA"
                     /locus_tag="OO309_000660"
     CDS             complement(2985..3440)
                     /gene="dksA"
                     /locus_tag="OO309_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR5845919.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3618..4322)
                     /gene="sfsA"
                     /locus_tag="OO309_000661"
     CDS             complement(3618..4322)
                     /gene="sfsA"
                     /locus_tag="OO309_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR5845920.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4337..4867)
                     /gene="thpR"
                     /locus_tag="OO309_000662"
     CDS             complement(4337..4867)
                     /gene="thpR"
                     /locus_tag="OO309_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR5845921.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4941..7370
                     /gene="hrpB"
                     /locus_tag="OO309_000663"
     CDS             4941..7370
                     /gene="hrpB"
                     /locus_tag="OO309_000663"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR5845922.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPKILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7566..10100
                     /gene="mrcB"
                     /locus_tag="OO309_000664"
     CDS             7566..10100
                     /gene="mrcB"
                     /locus_tag="OO309_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR5845923.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10320..12563
                     /gene="fhuA"
                     /locus_tag="OO309_000665"
     CDS             10320..12563
                     /gene="fhuA"
                     /locus_tag="OO309_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR5845924.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12614..13411
                     /gene="fhuC"
                     /locus_tag="OO309_000666"
     CDS             12614..13411
                     /gene="fhuC"
                     /locus_tag="OO309_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR5845925.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13411..14301
                     /gene="fhuD"
                     /locus_tag="OO309_000667"
     CDS             13411..14301
                     /gene="fhuD"
                     /locus_tag="OO309_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR5845926.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14298..16280
                     /gene="fhuB"
                     /locus_tag="OO309_000668"
     CDS             14298..16280
                     /gene="fhuB"
                     /locus_tag="OO309_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR5845927.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16315..17595)
                     /gene="hemL"
                     /locus_tag="OO309_000669"
     CDS             complement(16315..17595)
                     /gene="hemL"
                     /locus_tag="OO309_000669"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR5845928.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17820..19241
                     /gene="clcA"
                     /locus_tag="OO309_000670"
     CDS             17820..19241
                     /gene="clcA"
                     /locus_tag="OO309_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR5845929.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19265..19330
                     /locus_tag="OO309_000671"
     CDS             19265..19330
                     /locus_tag="OO309_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5845930.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19323..19667
                     /gene="erpA"
                     /locus_tag="OO309_000672"
     CDS             19323..19667
                     /gene="erpA"
                     /locus_tag="OO309_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR5845931.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19714..20337)
                     /locus_tag="OO309_000673"
     CDS             complement(19714..20337)
                     /locus_tag="OO309_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR5845932.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20375..21175)
                     /gene="btuF"
                     /locus_tag="OO309_000674"
     CDS             complement(20375..21175)
                     /gene="btuF"
                     /locus_tag="OO309_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR5845933.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21168..21866)
                     /gene="mtnN"
                     /locus_tag="OO309_000675"
     CDS             complement(21168..21866)
                     /gene="mtnN"
                     /locus_tag="OO309_000675"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR5845934.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21950..23467
                     /gene="dgt"
                     /locus_tag="OO309_000676"
     CDS             21950..23467
                     /gene="dgt"
                     /locus_tag="OO309_000676"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR5845935.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23597..25021
                     /gene="degP"
                     /locus_tag="OO309_000677"
     CDS             23597..25021
                     /gene="degP"
                     /locus_tag="OO309_000677"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR5845936.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25176..26332
                     /gene="cdaR"
                     /locus_tag="OO309_000678"
                     /pseudo
     CDS             25176..26332
                     /gene="cdaR"
                     /locus_tag="OO309_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26421..26807)
                     /locus_tag="OO309_000679"
     CDS             complement(26421..26807)
                     /locus_tag="OO309_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR5845937.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27121..27945)
                     /gene="dapD"
                     /locus_tag="OO309_000680"
     CDS             complement(27121..27945)
                     /gene="dapD"
                     /locus_tag="OO309_000680"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR5845938.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27976..30648)
                     /gene="glnD"
                     /locus_tag="OO309_000681"
     CDS             complement(27976..30648)
                     /gene="glnD"
                     /locus_tag="OO309_000681"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR5845939.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30710..31504)
                     /gene="map"
                     /locus_tag="OO309_000682"
     CDS             complement(30710..31504)
                     /gene="map"
                     /locus_tag="OO309_000682"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR5845940.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31872..32597
                     /gene="rpsB"
                     /locus_tag="OO309_000683"
     CDS             31872..32597
                     /gene="rpsB"
                     /locus_tag="OO309_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR5845941.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32855..33706
                     /gene="tsf"
                     /locus_tag="OO309_000684"
     CDS             32855..33706
                     /gene="tsf"
                     /locus_tag="OO309_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR5845942.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33853..34578
                     /gene="pyrH"
                     /locus_tag="OO309_000685"
     CDS             33853..34578
                     /gene="pyrH"
                     /locus_tag="OO309_000685"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR5845943.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34870..35427
                     /gene="frr"
                     /locus_tag="OO309_000686"
     CDS             34870..35427
                     /gene="frr"
                     /locus_tag="OO309_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR5845944.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35519..36715
                     /gene="ispC"
                     /locus_tag="OO309_000687"
     CDS             35519..36715
                     /gene="ispC"
                     /locus_tag="OO309_000687"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR5845945.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36904..37662
                     /gene="ispU"
                     /locus_tag="OO309_000688"
                     /gene_synonym="uppS"
     CDS             36904..37662
                     /gene="ispU"
                     /locus_tag="OO309_000688"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR5845946.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37675..38532
                     /gene="cdsA"
                     /locus_tag="OO309_000689"
     CDS             37675..38532
                     /gene="cdsA"
                     /locus_tag="OO309_000689"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5845947.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38544..39896
                     /gene="rseP"
                     /locus_tag="OO309_000690"
     CDS             38544..39896
                     /gene="rseP"
                     /locus_tag="OO309_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR5845948.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39926..42358
                     /gene="bamA"
                     /locus_tag="OO309_000691"
     CDS             39926..42358
                     /gene="bamA"
                     /locus_tag="OO309_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR5845949.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42480..42965
                     /gene="skp"
                     /locus_tag="OO309_000692"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42480..42965
                     /gene="skp"
                     /locus_tag="OO309_000692"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR5845950.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42969..43994
                     /gene="lpxD"
                     /locus_tag="OO309_000693"
     CDS             42969..43994
                     /gene="lpxD"
                     /locus_tag="OO309_000693"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR5845951.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44099..44554
                     /gene="fabZ"
                     /locus_tag="OO309_000694"
     CDS             44099..44554
                     /gene="fabZ"
                     /locus_tag="OO309_000694"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR5845952.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44558..45346
                     /gene="lpxA"
                     /locus_tag="OO309_000695"
     CDS             44558..45346
                     /gene="lpxA"
                     /locus_tag="OO309_000695"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR5845953.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45346..46494
                     /gene="lpxB"
                     /locus_tag="OO309_000696"
     CDS             45346..46494
                     /gene="lpxB"
                     /locus_tag="OO309_000696"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR5845954.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46491..47087
                     /gene="rnhB"
                     /locus_tag="OO309_000697"
     CDS             46491..47087
                     /gene="rnhB"
                     /locus_tag="OO309_000697"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR5845955.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47124..50606
                     /gene="dnaE"
                     /locus_tag="OO309_000698"
     CDS             47124..50606
                     /gene="dnaE"
                     /locus_tag="OO309_000698"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR5845956.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50619..51578
                     /gene="accA"
                     /locus_tag="OO309_000699"
     CDS             50619..51578
                     /gene="accA"
                     /locus_tag="OO309_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR5845957.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51677..53818
                     /gene="ldcC"
                     /locus_tag="OO309_000700"
     CDS             51677..53818
                     /gene="ldcC"
                     /locus_tag="OO309_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR5845958.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53875..54264
                     /locus_tag="OO309_000701"
     CDS             53875..54264
                     /locus_tag="OO309_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5845959.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54329..55627
                     /gene="tilS"
                     /locus_tag="OO309_000702"
     CDS             54329..55627
                     /gene="tilS"
                     /locus_tag="OO309_000702"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR5845960.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55676..55936)
                     /gene="rof"
                     /locus_tag="OO309_000703"
     CDS             complement(55676..55936)
                     /gene="rof"
                     /locus_tag="OO309_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR5845961.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55923..56123)
                     /locus_tag="OO309_000704"
     CDS             complement(55923..56123)
                     /locus_tag="OO309_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR5845962.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56289..56834
                     /locus_tag="OO309_000705"
     CDS             56289..56834
                     /locus_tag="OO309_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR5845963.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56831..57253
                     /gene="arfB"
                     /locus_tag="OO309_000706"
     CDS             56831..57253
                     /gene="arfB"
                     /locus_tag="OO309_000706"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5845964.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57267..57977
                     /gene="nlpE"
                     /locus_tag="OO309_000707"
                     /gene_synonym="cutF"
     CDS             57267..57977
                     /gene="nlpE"
                     /locus_tag="OO309_000707"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR5845965.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58132..58956)
                     /locus_tag="OO309_000708"
     CDS             complement(58132..58956)
                     /locus_tag="OO309_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR5845966.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59009..60727)
                     /gene="proS"
                     /locus_tag="OO309_000709"
     CDS             complement(59009..60727)
                     /gene="proS"
                     /locus_tag="OO309_000709"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR5845967.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60838..61545)
                     /gene="tsaA"
                     /locus_tag="OO309_000710"
     CDS             complement(60838..61545)
                     /gene="tsaA"
                     /locus_tag="OO309_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR5845968.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61542..61946)
                     /gene="rcsF"
                     /locus_tag="OO309_000711"
     CDS             complement(61542..61946)
                     /gene="rcsF"
                     /locus_tag="OO309_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR5845969.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62064..62879)
                     /gene="metQ"
                     /locus_tag="OO309_000712"
     CDS             complement(62064..62879)
                     /gene="metQ"
                     /locus_tag="OO309_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR5845970.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62919..63572)
                     /gene="metI"
                     /locus_tag="OO309_000713"
     CDS             complement(62919..63572)
                     /gene="metI"
                     /locus_tag="OO309_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR5845971.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63565..64596)
                     /gene="metN"
                     /locus_tag="OO309_000714"
     CDS             complement(63565..64596)
                     /gene="metN"
                     /locus_tag="OO309_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR5845972.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64784..65359
                     /gene="gmhB"
                     /locus_tag="OO309_000715"
     CDS             64784..65359
                     /gene="gmhB"
                     /locus_tag="OO309_000715"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR5845973.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            65723..>65979
                     /locus_tag="OO309_000716"
     rRNA            65723..>65979
                     /locus_tag="OO309_000716"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        15615 a        16340 c        18183 g        15841 t
ORIGIN      
        1 ctgcaaaaat gtttaaacta aaatttttat taaggaaaga gaggatgtat gattttcaga
       61 aaatatttat attcgtaata taaataaatt accatttgct taatttgtca aatgctcttg
      121 tatgtaagac ttcacgcaac gtctccccat caggaaacgc cagctccggt gcaatctcaa
      181 acagcggcca cagcataaat ccacgattct tcatatcgta gtgcggaacg gtcaggcgtt
      241 cggtatttat cacttcatta ccaaacagca tgatatcgag atccagcgtg cgtggtcccc
      301 agcgttcagc tttacgaaca cgaccctgtt gcagttctat gcgctgtgta tggttgagta
      361 actcttccgg ggcgggcgaa gtttcaagcg ccacggcggc gttgaggtaa tcgggttgat
      421 cttgcggccc cagcggtggg gtgcggtaaa acgaagaaac ggcaagaatg cggctttcag
      481 ggatatcgcc taatgctttc agggcagcat tgacctgctc cagcggagag gccagattgc
      541 tgcctatggc aatatacgcc actgtcatgt ggtaccctca cgacgcggtg cgcgtttgcg
      601 tggacgacga gtacgacgac gcggtgacgg ttcttcatcc agctcgttaa gcatcccttt
      661 ttgatctggt ggtgcggaaa cctggaactc accccaccat ttcaccagac gctgcagttc
      721 agcattacgt tcaacttcag ctcgcaaggc caacaggtca taagccgcac ggaacttagg
      781 atgctccagc agtttccatg cgcgtttacc ctgacgacgg gacatacgca actgcaactg
      841 ccagatatcg cgggttaacg tcgtcagacg tttcgggatt gccagtgaac ggcaggcttc
      901 gtccagcacg tcgttcatcg ccagcgcgaa agcgtcgtga taggtcaggc cgctttcctg
      961 ggcgatcttc tgtgccgtct ccagcagtgg gtaccagaac atggcggcaa acaagaacgc
     1021 cgggttcacg cgcatatcgt tatggatacg cgtatcggta ttcttcagca cctgttcaat
     1081 gatccgctcc atcgggctgt cgccattttc cgtgaagtag cgggtaatgg tcgggaacag
     1141 cggctggaac agatgatatt cacacaacag cttataggtt tcgtaaccgt agcccgcttg
     1201 tagcagttta agcgattctt caaacaggcg tgccggtggg atatcgttca gcagggtagc
     1261 gaggcgaggg atcggttctg cggtttccgg gctgatgcgc atacccaatt tggcggcaaa
     1321 acgtaccgcg cgcagcatac gtaccggatc ttcacggtag cgcgtttccg ggttaccaat
     1381 cagacggata acgccgtcct tcagatcctt cataccacca acgtaatcac ggacggtaaa
     1441 atccgctacg ctgtaataca ggctgttgat agtgaaatcg cggcgctggg cgtcttcttc
     1501 gatggagccg aaaatgttgt cgcgcagcaa catgccgttt tgcccgcgtt gggaggtcgt
     1561 gcggtcgctg acgttacctt cgtggtgtcc acggaaggtc gcaacttcga taatctccgg
     1621 gccaaacatt acatgagcca gacggaaacg gcgacccacc aggcggcagt tacggaacag
     1681 tttgcgcacc tgctcaggcg tggcgttagt ggttacgtca aaatctttcg gctttttgcc
     1741 aagtaacagg tcgcgcacgc cgccgccaac cagccaggct tcgtatcccg ctttgttgag
     1801 cctgtacatt accttcaggg cattttcact gatatctttg cgggaaatag catgctgctc
     1861 acgcgggatc accgtcacct gtggacgggc gactgcctgt tcagcctcgc tttcctcgcg
     1921 gcttagcacc ttgcggcaaa aattagcgac tcgggtaaaa atagtacacc tcggtagtgt
     1981 caaacatcat tcaggacaaa aaaatagcgg ctaatcatag ctcagcatga cgcatttgag
     2041 aatgttgaat ttacaattgc cgactcgggc acggcggtca gcatccagtt tttgacggct
     2101 gactgaagga tttgctcgac gctgaaatcc tgccagtgtg cttctgcctg ctgccccaga
     2161 aattgaagtg ccgcgattag taccgggcgt ggatcgcctt tcggcaacgc aggcgcatga
     2221 ttctgcttgg aaagtttagc gccttgtgga ttaagcgcca gcggcagatg aatgtaatct
     2281 ggcactttcc agccaaaaag ctggtacagc gggatttgcc ttactgttgg ttcaatcaga
     2341 tcagccccac gcactatttc tgtaacgccc tggaaatgat catcaaccac aacagccagg
     2401 ttgtaggcga acaacccatc acggcgatga atgataaaat cttcccgtgc cagtttttcg
     2461 tcggcgtgaa taatgcctcg cagcaggtca gtaaattgcg tgaccggatg ctgctggcgg
     2521 atacgcactg cggcgttgtc tggtccatga tgcaacaccc ggcaatgacc gtcgtaaata
     2581 ccgccaatgc tttgaatacg cgcacgcgta caggtgcagt aataacttag tccttgttca
     2641 tgtaaccagg cgagtgcttc acgataggcg tcgtgacgtt gcgattgcca gagaacatcg
     2701 ccgtcccagt gcagaccgta atgttccagc tggcgcagga tagtttctgc ggcaccggga
     2761 acttcacgag gcgggtcgat atcttctatg cgtaccagcc agcgaccttg ccgggcgcga
     2821 gcctgcaaat agctgccgag cgcggcgatc agagagccaa aatgaagctc gccggaagga
     2881 gagggggcga agcggccaat atactgtgtg tctgtcatct ctttgaacaa aaaataaggc
     2941 gggagcattt cccgcctgtg gtaaacgtga tggaacggct gtaattagcc agccatctgt
     3001 ttttcgcgaa tttcagccag cgttttgcag tcgatgcaca gatcggctgt cgggcgcgct
     3061 tccagacggc gaataccaat ttcaacaccg caggattcgc agtagccgaa atcttcgtct
     3121 tccacttttt tcagcgtctt ctcgatcttt ttgatcagct tacgctcgcg atcgcggtta
     3181 cgcagttcga ggctgaactc ttcttcctgg gctgcacggt ctaccgggtc cgggaagttg
     3241 gctgcttcat cctgcatatg tgtaacggtg cgatcgactt catccctgag ttgattacgc
     3301 catgcttcca gaatacgacg gaagtgcgcc agctgggctt cattcatata ctcttcgccc
     3361 ggcttctcct gatatggttc caccccagcg atggcgagaa tactcaggga cgatgtttta
     3421 cggttttgcc cttcttgcat gttgcttctc cttaacacgc actatcgatc cccatgttcg
     3481 ggggaaaaat gaggccgcta taaatagcag atgcttttcc ggatagcaat tatctaaacg
     3541 taacacttga caactgtgtg aggaaaagcg tatttgcgca cgcgaccaga atgtaaatta
     3601 accagttact tactttacta caatgtaacc ggcagtgatt ttttaagagc catgccttca
     3661 gcagaaattt ccgctttgta agccagaatt tctacccccc tctgttgagc ttctgacaat
     3721 agttgcgcgt atttctcatc gatgtggcgc gcgggtgaaa accgtgtaat ggctgaatgc
     3781 agcacggcga aaaaaataac cgcacgctgg ccttcagccg ctacgctcat caactcccga
     3841 aggtgtttct gacctcgttc agtgaccgca tcgggaaaat atccctgttc gttctccgct
     3901 aacgtaaccg atttcacttc aatatagcag tctggacgcg aatccgcctg caacataaag
     3961 tcaatacggc tgcgttcgga gccgtatttt acttcgcttt tcagcgagct atagcctgac
     4021 agttctgaaa ttgattcatt aaggatagcc tctttcgtca acctgttagc ccaaagcgta
     4081 ttgacgcaaa taaatgcgcc gctctggctt tgagttaatt cccaggtgtg tgggtatttc
     4141 cgtttggtgt tgtctgaagt cgaataccag acggtatcgc caggcgttgc acaaccggtc
     4201 atcgcacccg tattcgggca gtgtagcgtt aattcgcgac catcgggtgt gatcacatcg
     4261 gctaaaaaac gtttgtaacg ctgaattagc gtcgcgcgct gtagaggggg agaaaattcc
     4321 attgcgacaa tccttgttat tgcgttagcg cccagcgttt tagcggcgtg tagcgtgtgc
     4381 gtccacgggc aaacgaggag gcgtaaaggg tgaactccgt caccgcataa gaccagttaa
     4441 aacccggcgg cgggaatgtc accgcctcgc tggcgtcgcg caataaggta atatgtggat
     4501 gaaacggacg attgctttga aaacaaccgc tgcgggcagc ctgtgaacgg agcatattcg
     4561 ccagctggat taagccgcgt ggaggctgac gcatccctaa ccacaccaca cgcgaacgca
     4621 gccattgtcc ggcgtcatca agcgtgagtg tgaaaccagg ttgacgaatc cgtccggcta
     4681 aaagagaaag cgccttctct ttctctgcgc tcacttcgcc taaaaatgcc agagtcaggt
     4741 gcaaattatc ggcggcgacc ggacgtcccg cctcaggtgg gaagtgtgtg gcgcgccaat
     4801 ggataatctg ttcgcggatt tctgcaggta agtcgatagc aaagaacaga cgttgcggtt
     4861 cagacatgtg aggcactcgg ttatgaatta ccgcgatgct acaatgtggc gcgaagaatg
     4921 ttaaccctct ggagcgtttt gtgtcgtcgt tgcccgttgc tgccgtctta cctgaattac
     4981 ttaccgctct cgattgtgcg ccgcaggtat tattaagtgc gccgaccggg gccgggaaat
     5041 caacctggct gccgctgcaa ctgctggcgc atcccggcat taacgggaaa attatcctgc
     5101 tggagccgcg tcgtctggcg gcgcgtaacg tcgcgcaacg gctggcggag ctgcttaacg
     5161 aaaagccagg cgataccgtt ggctaccgga tgcgtgcgca aaactgcgtc gggctgaata
     5221 cccgcctgga agtggttacc gaaggcgtgc tgacgcggat gatccagcgt gacccggaac
     5281 tgagcggtgt tggattggtg atccttgatg agtttcatga gcgcagtttg caggcggatt
     5341 tggcgttggc gctgttactc gatgtgcaac aaggtctgcg tgatgacctt aaactgctga
     5401 ttatgtcggc tacgctggac aatgaccgct tgcagcaaat gctgccggaa gcgcccgtcg
     5461 tcatctcaga agggcgctcg tttccggttg aacgccgtta tttaccgctg cccgcgcatc
     5521 agcgttttga cgaagccgtt gcggtagcca ccgccgaaat ggtgcgtcag gaaagtggat
     5581 cattactgtt atttttacct ggcgtcggag aaattcagcg cgtgcaggaa caactggctt
     5641 cgcgcatcgg cagtgatgtg gtgctctgcc cgctgtatgg cgtgttgtca ctgaacgatc
     5701 agcgaaaagc gatcctcccg gcaccgcaag ggatgcgcaa agtggtgctg gcgaccaata
     5761 ttgctgaaac cagtttaacc atcgaaggta ttcgtctggt ggtggattgt gcccaggagc
     5821 gtgtggcgcg ctttgatccg cgcacggggc ttacgcggtt gattactcaa cgcgttagtc
     5881 aggcatccat gacgcagcgt gccgggcgcg ccgggcgtct ggagccgggt atcagcctgc
     5941 atttaatcgc caaagaacaa gcagaacgcg ccgcggcgca aagtgagccg aagatcttac
     6001 aaagcgatct ttccggtttg ctgatggaat tactgcaatg gggatgcagc gatccggcgc
     6061 agatgagctg gctggatcaa ccgccaacgg tgaatctact ggccgcgaaa cgcctgttac
     6121 aaatgctggg ggcgctggag ggtgaacggc ttagtgcgca agggcaaaaa atggcagcgc
     6181 tgggtaacga tccgcgttta gcggcaatgc tggttaacgc aaagagcgat gacgaagctg
     6241 ctaccgcggc aaaaattgcc gccattctcg aagagccgcc acggatgggc aatagtgacc
     6301 tgggcgtggc gttttcgcgc aatcaaccag cctggcagca acgtagtcag caactgttaa
     6361 aacgcttaaa cgtacgcggc ggtgaggcag acagttcgct tatcgcgccg ctacttgccg
     6421 gggcgtttgc cgatcgcatt gctcatcgcc gtgggcaaga tggacgctat caactggcaa
     6481 acagcatggg agcgatgctc gatgccgacg acgcgctaag ccgccacgaa tggttgatcg
     6541 caccgttatt attgcagggc agcgcctcgc cggatgcgcg gattttactg gcgctaccgg
     6601 tcgatattga tgaattagta caacgctgtc cgcagctggt gcagcaatct gacaccgtgg
     6661 aatgggatga cgcgcaaggt acgctgaaag cctggcgtcg gctacaaatc ggtcagttga
     6721 cggtgaaagt gcagccgctg gcgaaaccgt cagaagacga gttgcatcag gcgatgctta
     6781 atggcatccg tgataaaggt ttaagcgtgc tcaactggac ggcggaagcg gaacagctac
     6841 gcttgcgttt gttatgcgcc gcaaagtggt tgccggaata tgactggcca gcggttgatg
     6901 atgaaagttt gttggcgacg ctggaaacgt ggctgctgcc acatatgacc ggcgtacatt
     6961 cactacgcgg cctgaaatca ctcgacattt atcaggcact acgcggatta cttgattggg
     7021 gaatgcagca acgtctggat agtgaattgc ctgcgcatta cactgtgccg acgggaagcc
     7081 ggatcgccat tcgttatcat gaagataacc cgcccgcgct ggcggtgaga atgcaggaga
     7141 tgtttggtga ggccactaat ccgacgatcg cccaggggcg cgtgccgctg gtgctgaagt
     7201 tgctttcccc tgcccaaagg ccgctgcaaa tcacgcgtga tttgggggcc ttctggaaag
     7261 gggcgtatcg tgaggtgcaa aaggagatga aagggcgtta tcccaaacac gtctggccgg
     7321 acgacccggc aaacacggca ccgacgcgac ggacgaaaaa gtattcgtaa ggttggtttt
     7381 ctccctctcc ctgtgggaga gggccggggt gagggcatca gcgcgcacgt tcaccctcac
     7441 cctaaccctc tccctcaagg gagaggggac cgatcgagcg caaattttga gagatatctt
     7501 cttctgtctt gtaacagaag aacagaaaat cgggcttttg cgcctgaata ttgcggagaa
     7561 aaagcatggc cgggaatgac cgcgagccaa ttggacgcaa agggaaaccg acgcgtccgg
     7621 tcaaacaaaa ggtaagccgt cgtcgttacg aagatgacga tgattacgac gattatgatg
     7681 actatgagga tgaagaaccg atgccgcgca aaggtaaggg caaaggcaaa gggcgtaagc
     7741 ctcgtggcaa acgcggctgg ctatggctac tgctaaaact ggctatcgtt tttgccgtgc
     7801 tgatcgccat ttacggcgtt tatctcgatc aaaaaattcg tagccgtatt gatggcaagg
     7861 tctggcaact gcccgcggca gtttatggcc gaatggtcaa ccttgaacca gatatgacca
     7921 tcagcaagaa cgaaatggtg aagctgctgg aggcaacaca gtatcgtcag gtgtcgaaaa
     7981 tgacccgtcc tggcgaattt accgtgcagg ccaacagcat tgagatgatc cgccgtccgt
     8041 ttgatttccc ggacagtaaa gaagggcagg tgcgtgcgcg tctgaccttt gatggtgatc
     8101 atctggcgac catcgtcaat atggagaaca accgtcagtt cggtttcttc cgtcttgatc
     8161 cgcgtctgat caccatgatc tcctcgccaa acggtgagca gcgtttgttt gtgccgcgca
     8221 gtggtttccc tgatttgctg gtggatactt tgctggcgac agaagaccgt catttttacg
     8281 agcatgatgg aatcagtctc tactccattg ggcgtgcggt gctggcaaac ctgaccgcag
     8341 gtcgcacggt gcagggtgcg agtacgctga cgcaacagct ggtgaaaaac ctgttcctct
     8401 ccagcgagcg ttcttactgg cgtaaagcga acgaagctta catggcgctg atcatggacg
     8461 cgcgttacag caaagaccgt attcttgagc tgtatatgaa cgaggtgtat ctcggtcaga
     8521 gcggcgacaa cgaaatccgc ggcttcccgc tggcaagctt gtattacttt ggtcgcccgg
     8581 tagaagagct aagcctcgac cagcaggcgc tgttagtcgg tatggtgaaa ggggcgtcca
     8641 tctacaaccc gtggcgtaac ccgaaactgg cgctggagcg acgtaatctg gtgctgcgtc
     8701 tgctgcaaca gcaacagatt attgatcaag aactctatga catgttgagt gcccgtccgc
     8761 tgggggttca gccgcgtggt ggggtgatct ctcctcagcc agcctttatg cagctggtgc
     8821 gtcaggagtt gcaggcaaaa ctgggcgata aggtaaaaga tctctccggc gtgaagatct
     8881 tcactacctt tgactcggtg gcccaggacg cggcagaaaa agccgccgtg gaaggcattc
     8941 cggcactgaa gaaacagcgt aagttgagcg atcttgaaac tgcgattgtg gtcgtcgacc
     9001 gctttagtgg tgaagttcgt gcgatggtcg gaggttctga gccgcagttt gcgggctaca
     9061 accgtgcgat gcaggcgcgt cgttcgattg gttcccttgc aaaaccagcg acttatctga
     9121 cggctttaag ccagccgaaa atctatcgcc tgaacacgtg gattgccgat gcgccaatcg
     9181 cgctgcgtca gccgaatggc caggtctggt caccgcagaa cgatgaccgt cgttatagcg
     9241 aaagcggcag agtgatgctg gtggatgcgt tgacacgttc gatgaacgtg ccgacggtaa
     9301 atctggggat ggcgctgggg ctgcctgcgg ttacggagac ctggatcaaa ctgggcgtac
     9361 cgaaagatca gttgcatccg gttccggcaa tgctgctggg ggcgttgaac ttaacgccaa
     9421 tcgaagtggc gcaggcattc cagaccatcg ccagcggtgg taaccgtgca ccgctttctg
     9481 cactgcgttc ggtaatcgcg gaagatggta aggtgctgta tcagagcttc ccgcaggcag
     9541 aacgcgctgt tccggcgcag gcggcgtatc tgacactatg gaccatgcag caggtggtac
     9601 aacgtggtac gggtcgtcag cttggggcga aatacccgaa cctgcatctg gcagggaaaa
     9661 cagggactac caacaataac gtagatacct ggtttgcagg cattgacggc agcacggtga
     9721 ccatcacctg ggtaggccgt gataacaacc agccgaccaa actgtatgga gccagcgggg
     9781 caatgtcgat ttatcagcgt tatctggcta accagacgcc aacgccgctg aatcttgttc
     9841 cgccagaaga tattgcagat atgggcgtgg actacgacgg caactttgtt tgcagcggtg
     9901 gcatgcgtgt cttgccggtc tggaccagcg atccgcaatc gctgtgccag cagagcgaga
     9961 tgcagcagca gccgtcaggc aatccgtttg atcagtcttc tcagccgcag caacagccgc
    10021 aacagcaacc tgctcagcaa gagcagaaag acagcgacgg tgtagccggt tggatcaagg
    10081 atatgtttgg tagtaattaa catctaagcg tgaaataccg gatggcgagt tgccatccgg
    10141 taaaataaca tcccatctaa gatattaacc ctttcttttc atctggttgt ttattaaccc
    10201 ttcaggaacg ctcagattgc gtaccgcttg cgaacccgcc agcgtttcga atattatctt
    10261 atctttataa taatcattct cgtttacgtt attattcact ttacatcaga gatataccaa
    10321 tggcgcgttc caaaactgct cagccaaaac actcactgcg taaaatcgca gttgtagtag
    10381 ccacagcggt tagcggcatg tctgtttatg cacaggcagc ggttgaaccg aaagaagaca
    10441 ctatcaccgt taccgctgca cctgcgccgc aagaaagcgc atgggggcct gctgcaacta
    10501 ttgcggcgcg gcagtcagct accggcacta aaaccgatac gccgattcaa aaagtgccac
    10561 agtctatttc tgttgtgacc gccgaagaga tggcgctgca tcagccgaag tcggtaaaag
    10621 aagcgcttag ctacacgccg ggtgtctctg ttggtacgcg tggcgcatcc aacacctatg
    10681 accacctgat cattcgcggt tttgcggcag aaggccaaag ccaaaataac tatctgaatg
    10741 gcctgaagtt gcagggcaac ttctataacg atgcggtcat tgacccgtat atgctggaac
    10801 gcgctgaaat tatgcgtggc ccggtttccg tgctttacgg taaaagcagt cctggcggtc
    10861 tgttgaatat ggtcagcaag cgtccgacca ccgaaccgct gaaagaagtt cagtttaaag
    10921 ccggtactga cagcctgttc cagactggtt ttgactttag cgatgcgttg gatgatgacg
    10981 gtgtttactc ttatcgcctg accggtcttg cgcgttctgc caatgcccag cagaaagggt
    11041 cagaagagca gcgttatgct attgcaccgg cgttcacctg gcgtccggat gataaaacca
    11101 atttcacctt cctttcttac ttccagaacg agccggaaac cggttattac ggctggttgc
    11161 cgaaagaggg aaccgttgag ccgctgccga acggtaagcg tctgccgaca gactttaatg
    11221 aaggggcgaa gaacaacacc tattctcgta atgagaagat ggtcggctac agcttcgatc
    11281 acgaatttaa cgacaccttt actgtgcgtc agaacctgcg ctttgctgaa aacaaaacct
    11341 cgcaaaacag cgtttatggt tacggcgtct gctccgatcc ggcgaatgct tacagcaaac
    11401 agtgtgcggc attagcgcca gcggataaag gccattatct ggcacgtaaa tacgtcgttg
    11461 atgatgagaa gctgcaaaac ttctccgttg atacccagtt gcagagcaag tttgccactg
    11521 gcgatatcga ccacaccctg ctgaccggtg tcgactttat gcgtatgcgt aatgacatca
    11581 acgcctggtt tggttacgac gactctgtgc cactgttcaa tctgtacaat ccggtgaatc
    11641 ccgatttcga cttcaatgcc aaagatccgg caaactccgg cccttaccgc attctgaata
    11701 aacagaaaca aacgggcgtt tatgttcagg atcaggcgca gtgggataaa gtgctggtca
    11761 ccctaggcgg tcgttatgac tgggcagatc aagaatctct taaccgcgtt gctgggacga
    11821 ccgataaacg tgatgacaaa cagtttacct ggcgtggtgg tgttaactac ctgtttgata
    11881 atggtgtaac accttacttc agctatagcg aatcgtttga accttcttcg caagttggga
    11941 aggatggtaa tattttcgca ccgtctaaag gtaagcagta tgaagtcggc gtgaaatatg
    12001 taccggaaga tcgtccgatt gtagttactg gtgccgtgta taatctcact aaaaccaaca
    12061 acctgatggc ggaccctgag ggttccttct tctcggttga aggtggcgag atccgcgcac
    12121 gtggcgtaga aatcgaagcg aaagcggcgc tgtcggcgag tgttaacgta gtcggttctt
    12181 atacttacac cgatgcggaa tacaccaccg atactaccta taaaggcaat acgcctgcac
    12241 aggtgccaaa acacatggct tcgctgtggg ctgactatac cttctttgac ggtccgcttt
    12301 caggtctgac gctgggcacc ggtggtcgtt atactggctc cagctatggt gatccggcta
    12361 actcctttaa agtgggaagt tatacggtcg tggatgcgtt agtgcgttat gatctggcgc
    12421 gagtcggcat ggcgggctcc aacgtggcgc tgcatgttaa caacctgttc gatcgtgaat
    12481 acgtcgccag ctgctttaac acttatggct gcttctgggg cgcagaacgt caggtcgttg
    12541 caaccgcaac ctttcgtttc taatttctct tttggggcac ggatttccgt gcccatttca
    12601 caagttggct gttatgcagg aatacacgaa tcattccgat accacttttg cactgcgtaa
    12661 tatctccttt cgtgtgcccg ggcgcacgct tttgcatccg ctgtcgttaa cctttcctgc
    12721 cgggaaagtg accggtctga ttggtcacaa cggttctggt aaatccactc tgctcaaaat
    12781 gcttggtcgt catcagcctc cgtcagaagg ggagattctt cttgatgccc agccgctgga
    12841 aagctggagc agcaaagcgt ttgcccgcaa agtggcttat ttgccgcagc agcttcctcc
    12901 ggcagaaggg atgaccgtgc gtgaactggt ggcgattggt cgttacccgt ggcatggcgc
    12961 gctggggcgc tttggtgcgg cagatcgcga aaaggtcgag gaagctatct cgctggttgg
    13021 cttaaaaccg ctggcgcatc ggctggtcga tagtctctct ggcggcgaac gtcagcgggc
    13081 gtggatcgcc atgctggtgg cgcaggatag ccgttgtctg ttgctcgacg aaccgacttc
    13141 agcgctggat atcgcccacc aggttgatgt gctggcgctg gtgcaccgtt taagtcagga
    13201 acgtggcctg acggtcattg ccgtgttgca cgatatcaat atggcggcac gctactgtga
    13261 ttatctggtc gccctgcgca gtggtgaaat gattgctcag ggaacgcctg cggaaattat
    13321 gcgcggcgaa accctcgaaa tgatttatgg catcccgatg ggtattttgc cgcatccggc
    13381 gggtgctgca cctgtgagtt ttgtttattg atgagcggct tacctcttat ttcgcgccgt
    13441 cgactgttaa cggcgatggc gctttctccg ttgttatggc agatgaatac cgcccacgcg
    13501 gcggctattg accccaatcg tattgtggcg ctggagtggt tgccggtgga attactgctg
    13561 gcgctcggca tcgtgcctta cggcgtggcg gataccatca actatcgcct gtgggtcagc
    13621 gaaccaccat tgccggactc agtgattgac gtcggtttgc gtacagaacc taaccttgaa
    13681 ctgctgaccg aaatgaaacc gtcgtttatg gtctggtcgg caggatatgg cccttcatca
    13741 gaaatgctgg cacgtattgc gccgggtcgc ggatttaact tcagtgacgg caaacatccg
    13801 ttggcgatgg cgcgtaaatc gctgacggaa atggcagatt tacttaacct gcaaagcgca
    13861 gcggaaacgc atttagcgca atacgaagac tttatccgca gcatgaaacc ccgctttgtg
    13921 aagcgtggtg cacgcccgtt actgctgacg acgcttatcg atccgcgcca tatgctggtc
    13981 ttcggtccaa acagcttgtt ccaggaaatt ctcgatgagt acggcatccc aaatgcctgg
    14041 caaggggaaa ccaacttctg gggcagtacc gccgtcagta tcgatcgtct ggcagcgtat
    14101 aaagacgttg atgtgctctg ttttgatcac gacaacagca aagacatgga tgcgctaatg
    14161 gcaaccccgc tgtggcaggc catgccgttt gtccgtacag ggcgctttca gcgcgtaccc
    14221 gcagtctggt tttatggtgc aacgctttcg gcaatgcact ttgtgcgtgt tctggataac
    14281 gccatcggag gtaaagcgtg agtaaacgaa ttgcgctttt cccggcgtta ttgctggcgc
    14341 tgttagtgat tgtcgctacg gcgctcacct ggatgaactt ctcacaggcg ctgccgcgca
    14401 gtcagtgggc gcaggccgcc tggtcgccgg atattgacgt catcgagcag atgatttttc
    14461 actacagctt gttgccgcgt ctggcgattt cgctgctggt gggcgcgggc ctggggctgg
    14521 tgggcgtgct gtttcagcaa gtactgcgta acccgctggc ggaaccgacg acgttgggcg
    14581 ttgcaacagg cgcgcaactg gggattaccg tcaccacgct ctgggcgatc cctagcgcga
    14641 tggcgagcca gtttgctgcg ctggcagggg cttgcgttgt tggcttaatt gtctttggcg
    14701 tcgcgtgggg gaaacggctg tcgccggtaa cgctgatttt cgcggggttg gtagtgagcc
    14761 tttattgcgg cgcaatcaat cagttactgg ttatcttcca tcatgaccag ttgcaaagca
    14821 tgtttctgtg gagcactgga acgctgacgc aaaccgactg gggcggtgtt gagcgtttat
    14881 ggccgcagtt gctgggcggt gtgatgctga cgttgctgct acttcgtccg ttaaccctga
    14941 tggggcttga tgatggcgtg gcgcgcaatc tcgggctggc cttgtcgctt gcgcgtctgg
    15001 cggcgctgtc gctggcgatt gtcatcagtg cgctgctggt gaacgctgtg gggattatcg
    15061 gctttatcgg gttgttcgcg ccgctgctgg caaaaatgct gggggcgcgg cgtctgctgc
    15121 cacgactgat gctggcgtcg ttgattggtg cgctgatcct ctggctttcc gatcaaatca
    15181 tcctctggct gactcgcgtg tggatggaag tgtccaccgg ttcggtcact gcgttgatcg
    15241 gtgcgccgct gctactgtgg ctgctgccgc gtttacgcag cattagcgcg ccggatatga
    15301 aggttaacga tcgtgtcgcg actgaacgcc aacatgtgct ggcgtttgcc ctcgcgggcg
    15361 gcgtgctgtt gttgatggct gtggtggtgg cgctgtcgtt tggtcgtgat gcgcacggct
    15421 ggacgtgggc gagcggggcg ttgctcgagg atttaatgcc ctggcgctgg ccgcgaatta
    15481 tggcggcgct gtttgcgggc gtcatgctgg cggtagcggg ctgtattatt cagcgactga
    15541 ccggcaaccc gatggcaagc ccggaagtgc tggggattag ctccggcgcg gcgtttggtg
    15601 tggtgttaat gctgtttctg gtaccgggta atgcctttgg ctggttgtta cccgcaggca
    15661 gtctcggggc ggcggtgacg ctgttgatca ttatgatcgc cgccggacgc ggtggatttt
    15721 ccccacaccg catgttactg gcggggatgg cgttaagtac cgcgttcacc atgcttttga
    15781 tgatgttgca ggcaagtggt gacccgcgaa tggcgcaagt gctgacctgg atttccggtt
    15841 cgacctacaa cgcgaccgat gctcaggtct ggcgcaccgg aattgtgatg gtgattttgc
    15901 tggcgattac tccgctgtgc cgccgctggc tgaccatttt accgctgggt ggtgataccg
    15961 cgcgggcggt agggatggcg ctgacgccga cgcgaattgc gctgctgctg ttagcggctt
    16021 gcctgacggc gaccgcgacg atgactattg gaccgttgag ttttgttggt ttaatggcac
    16081 cgcatattgc gcggatgatg ggctttcgac ggacgatgcc acacatcgta atttcggcgc
    16141 tggtgggtgg tttactgctg gtgttcgctg actggtgtgg gcggatggtg ctgtttccat
    16201 tccagatccc ggcggggctg ctgtcgacct ttatcggtgc gccatatttt atctatttgc
    16261 tgagaaagca gagccgttaa tagaatgcct cgcccggtgg gcgaggcaaa attatcacaa
    16321 cttcgcgaac acccgacgtg cagcatcgat ggtgttattg atatcttcca tgctgtgcgc
    16381 cacagacata aagcctgctt caaacgctga cggtgccagg taaacacctt catccagcat
    16441 catatggaag aaacgcttaa aacgctcgac gtcacaggcc atcacatcct gatagcacgt
    16501 cacggactcg gcgtcggtaa agaagatacc gaacatacca ccgacgtggt taacgaccag
    16561 cggaattccc gcttcttctg ccgcttccag cagaccttct gccagacgtg ttgtcagctc
    16621 atccagcgtt tcgtgaatgc ccggctgcgc gacttcattc agacaggcga aacccgctgc
    16681 catcgcaatc gggttaccgg aaagcgtacc cgcctgatag accggacctg tcggggccag
    16741 cgcatccatt acatcacgac gaccaccgaa tgcgcctacc ggcattccac cgccgatgat
    16801 ttttccgagg caggttaaat ctggcactac ggcgaaataa tcctgtgcgc cagctagcgc
    16861 tacgcggaaa ccggtcatca cttcatcgat gatcagcaac gcgccaaatt cgtcgcacag
    16921 cgcacgcaga cctggcagga actctggcag cggcggaaca cagttcatat tgcctgccac
    16981 cggctcgacg ataatacagg caatctcttg cgggtattgc tcaaacgcgg cgcgtacaga
    17041 ggccagatcg ttataagtac aggttaaggt atgtttggcg aaatctgccg gaacgcccgg
    17101 cgagtttggc tggcctaacg tgagtgcgcc agaaccggct ttcaccagca ggcagtcagc
    17161 gtgaccgtgg taacaacctt caaatttaat gattttgtcg cgaccggtaa aaccacgggc
    17221 caggcggatg gcgctcatcg tcgcctcggt gccggagttc accatgcgca ccatatccat
    17281 ggtcgggacc agttcggtca ccagttgcgc cattttcact tccatttcgg tcggtgcacc
    17341 aaagcttaaa ccacgctcgg cggcttcaat cacggcattg cggatcgccg gatggttatg
    17401 gcccagcacc atcggccccc aggaaccgac ataatcgata taggctttgc catcaacatc
    17461 gtacagataa gcgccgtccg ctttttcgat aaacagtgga gtgccgccca cgccagtaaa
    17521 ggcgcgaaca ggggagttca caccgccagg gatcagctcg cgcgctgcgc tgtaaagatt
    17581 ttcagactta ctcatggagg gttcctgatt cgtagaaaaa gtgaatggct gctattccat
    17641 gttattcata acaagttaaa tactcgtcaa acatcaggct gcttgtactg gtgcaatcct
    17701 gatttcttag agtataaaag ttttgtgcat ttgaaacatt acgctttgca aaggattttc
    17761 atggaacgtg cgagtaaaat gccgtcatct tatttgtatg accaataagt gatcattgga
    17821 tgaaaactga tactccctct ttagaaacac cgcaggccgc gcgcctgcga cgcagacaac
    17881 tgattcgcca acttcttgag cgcgataaaa ccccgttagc cattttgttt atggcggcag
    17941 tcgtcggcac gcttgtcggg ctggctgcgg ttgcttttga caaaggtgtc gcctggttgc
    18001 agaaccaacg tatgggggcg ctggtacata ctgctgataa ttatccgcta ctgttaaccg
    18061 tcgcttttct ctgttcggcg gtgctggcga tgttcggcta ctttttggtg cgcaaatacg
    18121 cgcccgaagc agggggatcg gggatcccgg aaattgaagg ggcgctggaa gatcaacgtc
    18181 ctgttcgctg gtggcgtgta ttgccggtga aattctttgg cgggctgggg acactcggcg
    18241 gaggcatggg gctagggcgc gaaggaccaa ccgtgcagat cggcggcaat atcagtcgca
    18301 tggtgcttga tattttccgc ctgaaaggtg atgaagctcg ccatacgctg ctggcaaccg
    18361 gtgctgctgc ggggctggct gcggccttta acgcgccgct ggcgggtatt ttgtttatta
    18421 tcgaagagat gcgtccgcag tttcgctata cgttaatttc gattaaagcg gtatttattg
    18481 gcgtcattat gtcgaccatt atgtaccgga tttttaatca tgaagttgcg ttgattgacg
    18541 tcggtaaact ttctgatgcg ccgcttaata cgctgtggct ttatctgatc ctcggtatta
    18601 tttttggcat tttcggccct atttttaata aatgggtgct ggggatgcag gatttgctgc
    18661 accgtgtgca cggcggcaat attaccaaat gggtactgat ggggggggcg attggcggtc
    18721 tgtgtggatt gctggggttt gtggcaccag caacgtcggg cggcggtttt aacctgattc
    18781 ctatcgctac cgcggggaat ttcagcatgg gaatgctggt gtttatcttc gtcgctcggg
    18841 tcattaccac cttactctgc ttctcttccg gcgcgccggg cggtattttt gccccgatgc
    18901 tggcgctggg tactgtgctg ggaaccgctt tcggaatggt tgccgttgag ctgtttccgc
    18961 aatatcacct ggaggcgggg acgtttgcta ttgccggaat gggggcatta ctggcggcat
    19021 ctattcgcgc gccgttaacg gggatcattc tggttctgga gatgaccgat aactaccagc
    19081 tcattttgcc aatgattatt accggtcttg gcgcaacact attagcgcaa tttaccggcg
    19141 ggaaacctct atactcggcg attcttgcgc gcacgctggc aaaacaggaa gctgagcaac
    19201 tggcgcgaag caaggccgca tcagccagcg agaatacttg aacgaaatac cagggtatta
    19261 gataatggcg attattattg ggttagaatt tgcccgattg ccgatgtcgt ttggagcaaa
    19321 atatgagtga tgacgtagca ctgccgctgg agtttaccga cgcagcagcc aacaaagtta
    19381 aaagcctgat cgctgacgaa gataacccga atctgaaatt acgcgtgtat atcaccggtg
    19441 gcggttgcag cggcttccag tatggtttca cctttgatga tcaggtgaac gaaggcgata
    19501 tgaccatcga aaaacagggc gttggcctgg tggttgatcc gatgagcctg caatatctgg
    19561 tcggcggttc cgttgactac accgaaggac tggaaggttc tcgtttcatc gtgaccaacc
    19621 cgaacgcgaa aagcacctgc ggttgcggtt cttcctttag catctaatct gttgttcatg
    19681 tgccggataa actatccggc acatcttctc tcttcaacgc ccattctcat ccagcgcaaa
    19741 cgtcggtagc ttaagatgcc aacgaatagc cgccagccga atcaatagcg tcacgaccat
    19801 gcccatcata ctggctgttt ccagtggtac ggaaaatgtg taataagccg tagcgtggac
    19861 aataccgccg ataatacagg cagttgcgta gatttctgta cgtaaaatca tggggatttc
    19921 gcggaccagt acatcacgaa tgatcccgcc gccaacgcca gtaatgacgc ccatacaaac
    19981 cgcgattaac ggaccggctt ccgcattaaa ggctttattc acgctaatgc cgacaaacac
    20041 cgccagacca acggcgtcca gcaccggcaa catccatttt ggtaagcgtc ttggctggcg
    20101 caccagcacg atggtcagca tgctggtgac catggcaacg accagatcgg tgggatcttt
    20161 cacccaaaat accgggccgt gatccagcgc catgtcgcga attgttccgt cgcctactgc
    20221 ggtaaccacg cccagtacca gaacaccaaa agggtccata cgcaattttc cggctaacaa
    20281 aacgccgtag atggcaaata ccgctgtgcc gactatatcc agccaataga cgagcattgt
    20341 tcaatcccca ctgagcacct gaaaaggtca ggcactaatc tacctgtgaa agcgcattac
    20401 agagctgttg tgctgcgagg ataatacgtg ggcttgcacg ttcaaaccag tcactcgtga
    20461 gaggaataac gggaattttg agctgttcac cccagtattg tttgatttta ggaatttggt
    20521 ccggtccgcc tgtaatgaca atagcctgtg gcgagcgtgc taacacctgt tcgcggctaa
    20581 cttgcggcca gggaacccgg ctgtctttaa agatgttttc tccgccacaa acttcgagta
    20641 cctggttctg aatcgactct tttccactgg taaatggcgg attaatgccg aattgcagaa
    20701 aaacacgttt tttaggttta tcagcatatt gcgctttcaa ttgcacgtac tgatccagca
    20761 gggattgcgc ggcttgttcg gccttgtctg gttgcggact ccagggggcc agttgacgta
    20821 acgcattggc aatttgttca atgcttgtcg catcgaccca catcactttt attcccagcg
    20881 aagccagctg gtcaacctgc cgctcggcat tacctccacg ccaggcaatc accagatcgg
    20941 gtttcagcgc gacaatgcgt tccagattca tcccctgcca ggtggaaacc tgctcaatct
    21001 tttgcgcttg tggaggatag tcggaatagc tgctgacccc aaccggcgtg atcccggcgg
    21061 caaaggcaag ttcagtgttg gcgggagaaa gcgtgatgac gcgcggcgcg gcgttgagcc
    21121 acagtggcgc gagaaaagac agggcgacca gcgccctgaa cagtgattta gccatgtgcc
    21181 agtttctgca ccagtgactc aaccatcagg ctggactgtt tagcggcaac agccaggaac
    21241 tcatcgaagc taagatgaga ctgttgatcg gccacgtcgg agatggcgcg tacgacaaca
    21301 aacgggacgt tgaaattgtg gcagacatgg gcgattgccg tcgcttccat ctctacagca
    21361 atggcctgtg ggaagttgtg gcggattttc gccagaccaa cagaaccgtt gatgaaagcg
    21421 tcgccgctaa caatcaggcc acgtacagcg ttaagattca gttcggcaat gcaggcctca
    21481 gcggcagcga tcagtttatc gtcagcttta aagcctgccg gacagcctgg taactgaccg
    21541 tattcataac caaatgccgt gacatccgcg tcgtgataac gcgcttcgtc cgagacaacg
    21601 atatcgccca ctttcagcgt tggtgccagg ccaccggcag aaccggtgtt aataatcaca
    21661 tctggcttgc agtgttccaa cagcagagtg gcacccagcg ccgcagcgac tttaccgatg
    21721 cccgatttca gaagcgcaac ctcggttcca ttcagttggc cggtatagat ttcgcaaccg
    21781 ccgagactga tagtttgacg gttttcgatt ttgtcacgca gcagcgtaac ttcttcttcc
    21841 attgcaccaa tgatgccgat tttcatagat ttactcgcga taagcccgat ttgaaggcat
    21901 agtttaccat gcgcttacgg ggaagcgtat ttctcacgcg ggagaggaca tggcacagat
    21961 tgatttccga aaaaaaataa actggcatcg tcgttaccgt tcaccgcagg gcgttaaaac
    22021 cgaacatgag atcctgcgga tcttcgagag cgatcgcggg cgtatcatca actctccggc
    22081 aattcgtcgt ctgcaacaaa agacccaggt ttttccactg gagcgcaatg ccgccgtgcg
    22141 cacgcgtctt acccactcga tggaagtcca gcaggtggga cgctacatcg ccaaagaaat
    22201 tttaagccgt ctgaaagagc ttaaattgct ggaagcatac ggcctggatg aactgaccgg
    22261 cccctttgaa agcattgttg agatgtcatg cctgatgcac gatatcggca atccgccgtt
    22321 tggtcatttt ggcgaagcag cgataaatga ctggtttcgc caacgtttgc acccggaaga
    22381 tgccgaaagc cagcctctga ctgacgatcg ctgcagcgtg gcggcactac gtttacggga
    22441 cggggaagaa ccgcttaacg agctgcggcg caagattcgt caggacttat gtcattttga
    22501 ggggaatgca caaggcattc gtctggtgca tacattgatg cggatgaatc tcacttgggc
    22561 acaggttggc ggtattttaa aatatacccg tccggcgtgg tggcgtggcg aaacgcctga
    22621 gacacatcac tatttaatga aaaagccggg ttattatctt tctgaagaag cctatattgc
    22681 ccgcttgcgt aaagaactta atttggcgct ttacagtcgt tttccattaa cgtggattat
    22741 ggaagctgcc gacgacatct cctattgtgt ggcagacctt gaagatgcgg tagagaaaag
    22801 aatatttacc gttgagcagc tttatcatca tttgcacgaa gcgtggggcc agcatgagaa
    22861 aggttcgctc ttttcgctgg tggttgaaaa tgcctgggaa aaatcacgct caaatagttt
    22921 aagccgcagt acggaagatc agttttttat gtatttacgg gtaaacaccc taaataaact
    22981 ggtaccctac gcggcacaac gatttattga taatctgcct gcgattttcg ccggaacgtt
    23041 taatcatgca ttattggaag atgccagcga atgcagcgat cttcttaagc tatataaaaa
    23101 tgtcgctgta aaacatgtgt ttagccatcc ggatgtcgag cagcttgaat tgcagggcta
    23161 tcgggtcatt agcggattat tagagattta tcgtccttta ttaagcctgt cgttatcaga
    23221 ctttactgaa ctggtagaaa aagaacgggt gaaacgtttc cctattgagt cgcgcttatt
    23281 ccacaaactc tcgacgcgcc atcggctggc ctatgtcgag gctgtcagta aattaccgtc
    23341 agattctcct gagtttccgc tatgggaata ttattaccgt tgccgcctgc tgcaggatta
    23401 tatcagcggt atgaccgacc tctatgcgtg ggatgaatac cgacgtctga tggccgtaga
    23461 acaataacca gtcttttgta aagacgaaca ataaattttt accttttgca gaaactttag
    23521 ttcggaactt cgggctataa aacgaatctg aagaacacag caattttgcg ttatctgtta
    23581 atcgagactg aaatacatga aaaaaaacac attagcactg agtgcactgg ctctgagttt
    23641 aggtttggcg ttatctccgc tctctgcaac ggcggctgag acttcttcag caacgacagc
    23701 ccagcagatg ccaagccttg caccgatgct cgaaaaggtg atgccttcag tggtcagcat
    23761 taacgtagaa ggtagcacaa ccgttaatac gccgcgtatg ccgcgtaatt tccagcagtt
    23821 cttcggtgat gattctccgt tctgccagga aggttctccg ttccagagct ctccgttctg
    23881 ccaggggggc cagggcggta atggcggcgg ccagcaacag aaattcatgg cgctgggttc
    23941 cggcgtcatc attgatgccg ataaaggcta tgtcgtcacc aacaaccacg ttgttgataa
    24001 cgcgacggtg attaaagtcc agctgagcga tggtcgtaag ttcgacgcga agatggttgg
    24061 caaagatccg cgctctgata tcgcgctgat ccaaatccag aacccgaaaa aactgaccgc
    24121 aattaagatg gcggattctg atgcactgcg cgtgggtgat tacaccgtag cgattggtaa
    24181 cccgtttggt ctgggcgaga cggtaacttc cgggattgtc tctgcgctgg ggcgtagcgg
    24241 cctgaatgct gaaaactacg aaaacttcat ccagaccgat gcagcgatca accgtggtaa
    24301 ctccggtggt gcgctggtta acctgaacgg cgaactgatc ggtatcaaca ccgcgatcct
    24361 cgcaccggac ggcggcaaca tcggtatcgg ttttgctatc ccgagtaaca tggtgaaaaa
    24421 cctgacctcg cagatggtgg aatacggcca ggtgaaacgc ggtgagctgg gtattatggg
    24481 gactgagctg aattccgaac tggcgaaagc gatgaaagtt gacgcccagc gcggtgcttt
    24541 cgtaagccag gttctgccta attcctccgc tgcaaaagcg ggcattaaag cgggtgatgt
    24601 gatcacctca ctgaacggta agccgatcag cagctttgcc gcactgcgtg ctcaggtggg
    24661 tactatgccg gtgggcagca aactgaccct gggcttactg cgcgacggta agcaggttaa
    24721 cgtgaacctg gaactgcagc agagcagcca gaatcaggtt gattccagct ccatcttcaa
    24781 cggcattgaa ggcgctgaga tgagcaacaa aggcaaagat cagggcgtgg tagtgaacaa
    24841 cgtgaagacg ggcactccgg ctgcgcagat cggcctgaag aaaggtgatg tgattattgg
    24901 tgcgaaccag caggcagtga aaaacatcgc tgaactgcgt aaagttctcg acagcaaacc
    24961 gtctgtgctg gcactgaaca ttcagcgcgg cgacagcacc atctacctgt taatgcagta
    25021 atctccctca accccttcct gaatacggga aggggttctc cttacaatct gtgaacttcg
    25081 ccacaactcc atacatcttc atcatccttt gggcatttgc acaatgccgt acgttacgta
    25141 cttccttatg ctaagccgtg cataacggag gacttatggc tggctggcat cttgatacca
    25201 aaatggcgca ggatatcgtg gcacgtacca tgcgcatcat cgataccaat atcaacgtaa
    25261 tggatgcccg cgggcgaatt atcggcagcg gcgatcgtga gcgtattggt gaattgcacg
    25321 aaggtgcatt gctggtactt tcacagggac gagtcgtcga tatcgatgac gcggtagcac
    25381 gtcatctgca cggtgtgcgg caggggatta atctaccgtt acggctggaa ggtgaaattg
    25441 tcggcgtaat tggcctgaca ggtgaaccag agaatctgcg taaatatggc gaactggtct
    25501 gcatgacggc tgaaatgatg ctggaacagt cgcggttgat gcacttgttg gcgcaggata
    25561 gccgtttgcg ggaagaactg gtgatgaacc tgattcaggc agaggagaat actcccgcac
    25621 ttctgaatgg gcgcaacggc tggggatcga tctcaatcaa ccgcgagtgg tggctattgt
    25681 tgaggtcgac agcggtcagc ttggcgtgga cagcgcaatg gcggagttac aacaactgca
    25741 aaacgcgctg actacgcccg agcgtaataa tctggtggcg attgtctcgc taaccgaaat
    25801 ggtggtgttg aaaccggcgt tgaactcttt tgggcgctgg gatgcagaag atcatcgtaa
    25861 gcgagttgaa caactgatta cccgcatgaa agagtacggc cagctgcgtt ttcgcgtttc
    25921 actgggcaac tattttaccg gtcctggcag tattgcccga tcctatcgta cggcgaaaac
    25981 gacgatggtg gtgggtaaac agcggatgcc agaaagtcgc tgctattttt atcaggatct
    26041 gatgttacct gtgttactcg acagtttgcg tggcgactgg caggccaacg aactggcgcg
    26101 accgctggcg cggctgaaag cgatggacaa taacggcttg ctgcgacgaa cgctggcggc
    26161 gtggtttcgc cacaatgtgc aaccgctggc aacgtcaaag gcgttgttta ttcatcgtaa
    26221 taccctggag tatcggctta atcgtatatc ggaactgacc gggcttgatt tgggcaattt
    26281 tgatgacagg ttgctgctgt atgtggcgtt acaactggat gaagagcggt agattatgcg
    26341 ttaaggttga ggcggcgctg gctcattccc ctcagcccga aggggagagg ggaatgcccg
    26401 agttaaccgt caattcggga ttatttacgc gttagttttt ccagatcggc ttcaatctcg
    26461 ctgatcttat tggttacgac tgactccagg tgacgtaagt cgtcgaggat cttacgctta
    26521 agatcaactt cgctgcggtc acgctgacag atttgatcaa gctcatcaat gatataccgt
    26581 agattcgggc tgatttcctg gacttcttta taaccctgac ccacaccatc agcgacgacc
    26641 gttttacgct gacgcggata tttaaactta acgctcttgg cgaaaaactc gcctttgtct
    26701 ttctggaaat agattttcag aatatcgttg ttggcttcct gccggaggct gtaacgatca
    26761 atttcttcag gattggtaat acccagactt ttcagattgt cgtacatagc gttacctcaa
    26821 aatgagtcag taaattgtgc ttattttagc atttggcttg gcccgcccgg ctcgactgtg
    26881 atcgcagaaa gcctttttct tccagacttc tatcatggcg taaaaatcaa aaaattacct
    26941 gctttattgt ggcgatattc catgggatgg attagcacat ttatcttcgt tttgcgaatt
    27001 acatgacagt ccgaaacaag atgaaaaagt atttaaaaaa gggggaggaa gaatgagtag
    27061 gacgaattat tctgagccag aaagcatacg ggcagcatca tgctgcccgt atatgtactc
    27121 ttaatcgatg gtacgcagca gttcgttaat gccgactttg ccgcgagttt tcgcgtcaac
    27181 tttcttaacg ataaccgcac agtagaggct gtatttgcca tcttttgacg gcagattacc
    27241 tgaaacaacc acagaccccg ccggaacgcg accgtagtgg atttcgccgg tttcacggtc
    27301 gtaaatacgg gtgctctgac caatgtatac gcccatagag atgacggaac cttcttcgac
    27361 aatcacccct tcaaccactt cagagcgtgc gccaatgaag cagttatctt caataatggt
    27421 cgggttagcc tgcagcggct ccagcacgcc gccgatgccc acgccaccgg aaaggtggac
    27481 gtttttacca atctgcgcac aagaaccgac ggtcgcccag gtatcaacca tggtgccttc
    27541 atcaacatat gcgccgatgt taacgtaaga cggcatcagc acggtgttac gggcaataaa
    27601 cgcaccctga cgtaccgccg ctggtggcac aacgcggaag ccttctttct ggaaacgtgc
    27661 ttcgtcgtag tcggcgaatt tcatcggcac tttgtcgaag tagcggcttt ctgccccttc
    27721 gatcacctga ttatcattaa tacggaaaga gagcagcacc gcttttttca accactgatg
    27781 cgtcacccac tgaccgtcaa ttttttccgc tacacgcagt gcgccggaat ccagcagggc
    27841 gatcacctga tttaccgctt cgcgggtaac ggtgtctgca ttggctggcg tgatctcggc
    27901 acggcgttca aaagcggttt caataatgtt ctgtaactgc tgcattgtta aactcttttc
    27961 atatcagtaa acacatcacc ctttatcgtt tggattgagg gcctctgtca accgctgatg
    28021 cacttcctgc tgcaactcgt tattaagcgc acgccggtca gcggtggcaa ttatgaataa
    28081 atcttctact cgctcgccaa tggttgtaat tcgggcacca tgaagcgaaa ttcccagatc
    28141 ggcaaaaatt ttcccgactc gcgccagcag tccaggttgg tcgagggcga tcagttcgag
    28201 gaacgatttg cggtcggtat gggtcggcaa aaacgttact tcggtttcaa cagtaaaatg
    28261 gcgtaatttg gcgggttggc gacggggctg cggtggctgc cagctacttt gcgtcagcac
    28321 ctgctccaga ccaaaccgaa taacctcatg gcgatctgcg gacagcgggc tgccatcggg
    28381 ttccagcacg ataaaggtat ccatcgccat accgtcgcga gtggtgaaaa tttgtgcgtc
    28441 gtgaacactt aaattgcggc ggtctaattc ggcacagacg gcggcaaaca gataggggcg
    28501 gtccgggctc cagataaaaa tctcggtgcc tccacgcgta gcctgcgggc taagcaatac
    28561 cagcggtttg cttaaatcat gctgtaataa atggcgggca tgccaggcca gttgatttgg
    28621 gctatggcgg acaaaatagt tagcacgaca gcgtgaccaa atttggtgca gcgcctcttc
    28681 gtcgatgtta tccatgcgca gtagtgccag tgcctggagt tggtgatggc gaacccgttc
    28741 gcgcatatcc ggcgtgtttt gcattccgcg tcgtagctgc ttttcggtgg caaaatagag
    28801 ttcgcgcaac agactttgct tccagctatt ccacagtgtt tcgttggtgg cgcaaatgtc
    28861 agccactgtc aggcatacca gatagcgcag acgattttcc gtttgaactt cttcggcaaa
    28921 ctgcttgatg acttcagggt cctgaatatc gcggcgttgt gctgtcaccg acatcagcaa
    28981 gtgctggcga accagccagg caaccagctg tgtttcgcgt gagttcagcc cgtggagttc
    29041 tgcaaaatgc actacatcct gagcaccgag aatggagtgg tcgccgccgc gtcctttggc
    29101 gatatcgtga aacagcgcgg cgatgaaaat cagctcagtt gacggcaggc gcggccagac
    29161 gtccacacac aacggatggc gctggcgcgt ttcttcactg gcaaaactct ccagtttcag
    29221 catcacgcgg atagtatgtt catccaccgt gtaggcgtgg aacagatcaa actgcatctg
    29281 cccgacgata tgcgaccatt gcggcatata cgcgccgagc acgctatggc gatgcattgg
    29341 caatagtccg cgccgcaccg ctccggggtg acgcagaatg ctcaaaaaca gttttcgtgc
    29401 ttgcggaata ttacacagcg gttgttgcag atggcgacgg gcatggcgta actggcgcag
    29461 cgtggtggag taaatgccgg tgatcgcact gttgcgcacc atggtgtaga acatacgcaa
    29521 gatggcttcc ggctggcgca taaatagtgt ttcatcacgc aggtcgatta gcgtaccgcg
    29581 tagctgaaac tcatcgtcga gtggacgtgg tttttcgtcg gcgggaaggg cgaggatggc
    29641 ttcatcgaac agttgcagca gcatctggtt gagttcactg acgcggcgtg taacgcggaa
    29701 gtaatccttc atcatccgct cgaccggttc gttgccttca ccactgtaat tcagacgctg
    29761 agcgacgcta agttggcgat cgaataacag acgattatcg taacggctga cgaccagatg
    29821 cagggcaaag cgaatacgcc acagtatatg cagacattcg tttaattccg cccgctccgc
    29881 tgaggttaag aagccaaacc cgaccatttc atccagcgat gttgcgccaa aatgacggcg
    29941 ggccacccat tgcagagtgt gaatatcgcg caagccgcca gggctacttt tgatgtctgg
    30001 ttcaaggttg tagctggtgc catggtaacg ctgatggcgc tggttctgtt cttcaacttt
    30061 cgctgcgtag aacttgtcgg aaggccagaa tccttcgcta aaaatatgtt tttgcagttc
    30121 gaggaagagc gcaacatcgc caattaataa gcgggattcg attaaattgg tggcgacggt
    30181 taaatccgat aacccttcca acatgcactc ttcaagcgtg cgcacactat gaccgacttc
    30241 cagctttaca tcccagagca gcgttaacag ctcgcccact ttttgcgcct gatcgtccgg
    30301 gagcttttta cggcttaaaa tcagtaaatc gatgtcagaa agtggatgca gctcgccacg
    30361 accgtagcca ccgacggcaa ccaatgccag gtcggcaatc tggctgaatc ccgcttcaat
    30421 ccataatcgt tgcaggagct ggtctatgaa ctcggtacgt gcctcaatca actgttctgc
    30481 agagatccca ttgtcaaagg catcacccag ccaacgctgg aaagtatcga tatgggcttt
    30541 tatcccacag acggttaatt catcacgggg ccaggcgcat ggattttgcg gttgaccggg
    30601 cagggtggag agagcggtgt ttgcgtactg ttctggaagg gtattcattg tgcgccaccc
    30661 ataagattaa attatcgcat taaaaaagcc ggcattcgcc ggcttcatct tattcgtcgt
    30721 gcgagattat cgccgggatg gtgtcatcct tgcgtagcgt caaaatttcg cagccgttat
    30781 cagtcaccac aatagtatgc tcatattgtg cagacaagct gcgatctttg gtttttaccg
    30841 tccagccatc tttcatggtg cggatctctt ttttacccgc gttgaccatt ggctcaatgg
    30901 tgaacgtcat ccccggtttc agcacgacgt tggtttcacg ggagtcatag tgcagcacct
    30961 gcggttcttc atggaagccg cgaccaatac cgtgtccgca atattcacga acgacggaga
    31021 agccttctgc ttcgacaaat ttctgaatcg ccgcaccgat ttcgcgcaga ttaatgcctg
    31081 gttttaccat gcgtagcgcc aggtacaggc tttcttgcgt gatgcggcac aggcgttcgc
    31141 ccatgatggt cggcttaccg acgataaaca ttttcgaggt atcgccgtgg aaaccatctt
    31201 tgattacggt gacatcaatg ttaacgatat cgccatcttt cagcagctta gcatcgtccg
    31261 ggataccgtg gcacaccact tcattaatag agatgcaaac ggatttcgga tagccgtgat
    31321 agccgaggca ggcagaaacc gcgtgttgtt cattaacaat gtaatcatta cagatgcgat
    31381 ccagctcgcc ggtgctgacg cccggtttaa catacggttc gatcatctcc agcacttcgg
    31441 cagccagtcg gccagcgacg cgcatttttt cgatatcttc tggggtcttg attgagatag
    31501 ccattaattc tgtccatcag cgtcggtgat accgacaata tatatgtaag tgccgtcaat
    31561 ggtatcacac ccgggcaaat tgagaatcat tctgaatttc gccaaacgtg ccactgaagg
    31621 ttttctataa tagaaaattc gacgtctgat gctgtacaca gcgccaacaa ttattggtgt
    31681 ccacgacgta tttgtggtat aaagcgcgcc ggacttccga tccatttcgt atacacagac
    31741 tggacggaag cgacaatctc actttgtgta acaacacaca cgtatcggca catattccgg
    31801 ggtgcccttt ggggtcggta atatgggata cgtggaggca taaccccaac ttttatatag
    31861 aggttttaat catggcaact gtttccatgc gcgacatgct caaggctggt gttcacttcg
    31921 gtcaccagac ccgttactgg aacccgaaaa tgaagccgtt catcttcggt gcgcgtaaca
    31981 aagttcacat catcaacctt gagaaaactg taccgatgtt caacgaagct ctggctgaac
    32041 tgaacaagat tgtttctcgc aaaggtaaaa tccttttcgt tggtactaaa cgcgctgcaa
    32101 gcgaagcggt gaaagacgct gctctgagct gcgaccagtt cttcgtgaac catcgctggc
    32161 tgggcggtat gctgactaac tggaaaaccg ttcgtcagtc catcaaacgt ctgaaagacc
    32221 tggaaactca gtctcaggac ggtactttcg acaagctgac caagaaagaa gcgctgatgc
    32281 gcactcgtga gctggagaaa ctggaaaaca gcctgggcgg tatcaaagac atgggcggtc
    32341 tgccggacgc tctgtttgta atcgatgctg accacgaaca cattgctatc aaagaagcaa
    32401 acaacttggg tattccggta tttgctatcg ttgataccaa ctctgatccg gacggtgttg
    32461 acttcgttat cccgggtaac gacgacgcaa tccgtgctgt gaccctgtac ctgggcgctg
    32521 ttgctgcaac cgtacgtgaa ggccgttctc aggatctggc ttcccaggcg gaagaaagct
    32581 tcgtagaagc tgagtaataa ggcttgataa ctcccccaaa atagttcgag ttgcagaaag
    32641 gcggcaagct cgagaattcc cgggagctta catcagtaag tgaccgggat gagcgagcga
    32701 agacaacgca tctgcggcgc gaaatatgaa gggggagagc ccttatagac caggtagtac
    32761 acgtttggtt agggggcctg catatggccc cctttttcac ttttatatct gtgcggttta
    32821 atgccgggca gatcacatct ccgaggattt tagaatggct gaaattaccg catccctggt
    32881 aaaagagctg cgtgagcgta ctggcgcagg catgatggat tgcaaaaaag cactgactga
    32941 agctaacggc gacatcgagc tggcaatcga aaacatgcgt aagtccggtg ctattaaagc
    33001 agcgaaaaaa gcaggcaacg ttgctgctga cggcgtgatc aaaaccaaaa tcgacggcaa
    33061 ctacggcatc attctggaag ttaactgcca gactgacttc gttgcaaaag acgctggttt
    33121 ccaggcgttc gcagacaaag ttctggacgc agctgttgct ggcaaaatca ctgacgttga
    33181 agttctgaaa gcacagttcg aagaagaacg tgttgcgctg gtagcgaaaa ttggtgaaaa
    33241 catcaacatt cgccgcgttg ctgcgctgga aggcgacgtt ctgggttctt atcagcacgg
    33301 tgcgcgtatc ggcgttctgg ttgctgctaa aggcgctgac gaagagctgg ttaaacacat
    33361 cgctatgcac gttgctgcaa gcaagccaga attcatcaaa ccagaagacg tatccgctga
    33421 agtggtagaa aaagaatacc aggtacagct ggatatcgcg atgcagtctg gtaagccgaa
    33481 agaaatcgca gagaaaatgg ttgaaggccg catgaagaaa ttcaccggcg aagtttctct
    33541 gaccggtcag ccgttcgtta tggaaccaag caaaactgtt ggtcagctgc tgaaagagca
    33601 taacgctgaa gtgactggct tcatccgttt cgaagtgggt gaaggcatcg agaaagttga
    33661 gactgacttt gcagcagaag ttgctgcgat gtccaagcag tcttaattat caaaaaggag
    33721 ccgcctgagg gcggcttctt tttgtgccca tcttgtaaat tcagctaacc cttgtggggc
    33781 tgcgctgaaa agcgacgtac aatgtcgcta gtattaattc atttcaatcg ttgacagtct
    33841 caggaaagaa acatggctac caatgcaaaa cccgtctata aacgcattct gcttaagttg
    33901 agtggcgaag ctctgcaggg cactgaaggc ttcggtattg atgcaagcat actggatcgt
    33961 atggctcagg aaatcaaaga actggttgaa ctgggtattc aggttggtgt ggtgattggt
    34021 gggggtaacc tgttccgtgg cgctggtctg gcgaaagcgg gtatgaaccg cgttgtgggc
    34081 gaccacatgg ggatgctggc gaccgtaatg aacggcttgg caatgcgtga tgcactgcac
    34141 cgcgcctatg tgaacgctcg tctgatgtcc gctattccat tgaatggcgt gtgcgacagc
    34201 tacagctggg cagaagctat cagcctgttg cgcaacaacc gtgtggtgat cctctccgcc
    34261 ggtacaggta acccgttctt taccaccgac tcagcagctt gcctgcgtgg tatcgaaatt
    34321 gaagccgatg tggtgctgaa agcaaccaaa gttgacggcg tgtttaccgc tgatccggcg
    34381 aaagatccaa ccgcaaccat gtacgagcaa ctgacttaca gcgaagtgct ggaaaaagag
    34441 ctgaaagtca tggacctggc ggccttcacg ctggctcgtg accataaatt accgattcgt
    34501 gttttcaata tgaacaaacc gggtgcgctg cgccgtgtgg taatgggtga aaaagaaggg
    34561 actttaatca cggaataatt cccgtgatgg ataaataagg gtaagattcc gcgtaagtat
    34621 cgcgggggcg taagtctggt tataaggcgt tattgttgca ggcagtttgg tcacggccag
    34681 cgcgcagcaa ccggagcgta caaaagtacg tgaggatggc gagcactgct cggggccaaa
    34741 atggcaaata aaatagccta ataatccaga cgattacccg taatatgttt aatcagggct
    34801 atacttagca cacttccact gtgtgtgact gtctggtctg actgagacaa gttttcaagg
    34861 attcgtaacg tgattagcga tatcagaaaa gatgctgaag tacgcatgga caaatgcgta
    34921 gaagcgttca aaacccaaat cagcaaaata cgcacgggtc gtgcttctcc cagcctgctg
    34981 gatggcattg tcgtggaata ttacggcacg ccgacgccgc tgcgtcagct ggcaagcgta
    35041 acggtagaag attcccgtac tctgaaaatc aacgtgtttg atcgttcaat gtctccggcc
    35101 gttgaaaaag cgattatggc ctccgatctc ggcctgaacc cgaactctgc gggtagcgac
    35161 atccgtgttc cgctgccgcc gctgacggaa gaacgtcgta aagatctgac caaaatcgtt
    35221 cgtggtgaag cagaacaagc gcgtgttgca gtacgtaatg tgcgtcgtga cgcgaacgac
    35281 aaagtgaaag cactgttgaa agataaagag atcagcgaag acgacgatcg ccgttctcag
    35341 gacgatgtac agaaactgac tgatgctgca atcaagaaaa ttgaagcggc gctggcagac
    35401 aaagaagcag aactgatgca gttctgattt cttgaacgac aaaaacgccg ctcagtagat
    35461 ccttgcggat cggctggcgg cgttttgctt tttattctgt ctcaactctg gatgtttcat
    35521 gaagcaactc accattctgg gctcgaccgg ctcgattggt tgcagcacgc tggacgtggt
    35581 gcgccataat cccgaacact tccgcgtagt tgcgctggtg gcaggcaaaa atgtcactcg
    35641 catggtagaa cagtgcctgg aattctctcc ccgctatgcc gtaatggacg atgaagcgag
    35701 tgcgaaactt cttaaaacga tgctacagca acaggggagc cgcaccgaag tcttaagtgg
    35761 gcaacaagcc gcttgcgata tggcagcgct tgaggaggtt gatcaggtga tggcagccat
    35821 tgttggtgct gctgggctgt tacctacgct tgctgcgatc cgcgcgggta aaaccatttt
    35881 gctggccaat aaagaatcac tggttacctg cggacgtctg tttatggacg ccgtaaagca
    35941 gagcaaagcg caattgttac cggtcgatag cgaacataac gctatttttc agagtttacc
    36001 gcaacctatc cagcataatc tgggatacgc tgaccttgag caaaatggcg tggtgtccat
    36061 tttacttacc gggtctggtg gccctttccg tgagacgcca ttgcgcgatt tggcaacaat
    36121 gacgcccgat caagcttgcc gtcatccgaa ctggtcgatg ggacgtaaaa tttctgtcga
    36181 ttcggctacc atgatgaaca aaggtctgga atacattgaa gcgcgttggc tgtttaacgc
    36241 cagcgccagc cagatggaag tgctgattca cccgcagtca gtgattcact caatggtgcg
    36301 ctatcaggac ggcagtgttc tggcgcagct gggggaaccg gatatgcgta cgccaattgc
    36361 ccacaccatg gcatggccga atcgcgtgaa ctctggcgtg aagccgctcg atttttgcaa
    36421 actaagtgcg ttgacatttg ccgcaccgga ttatgagcgt tatccatgcc tgaaactggc
    36481 gatggaggcg ttcgaacaag gccaggcagc gacgacagca ttgaatgccg caaacgaaat
    36541 caccgttgct gcttttcttg cgcaacaaat ccgctttacg gatatcgctg cgttgaattt
    36601 atccgtactg gaaaaaatgg atatgcgcga accacaatgt gtggacgatg tgttatctgt
    36661 tgatgcgaac gcgagtgaag tcgccagaaa agaggtgatg cgtctcgcaa gctgaggata
    36721 atccggctac agagagtcgc gctatttgtt agcgtagggc ttcagtgata tagtctgcgc
    36781 catctgatcg taagtagttg gctttataag gtcaggtatg ccgtggtttt acacggcttt
    36841 tttttgtata ggcttcagta ttcctgagta ccgtaaaccc tgtcagggaa taaaaaacgc
    36901 gtgatgttgt ctgctactca accacttagc gaaaaattgc cagcgcatgg ctgccgtcat
    36961 gttgcgatca ttatggacgg caatggtcgc tgggcaaaaa agcaagggaa gattcgtgcc
    37021 tttgggcata aagccggggc aaaatccgtc cgccgggctg tctcttttgc ggccaacaac
    37081 ggtattgagg cgttaacgct gtatgccttt agtagtgaaa actggaaccg accagcgcag
    37141 gaagtcagtg cgttaatgga actgtttgtg tgggcgctcg atagcgaagt aaaaagtctg
    37201 caccgacata acgtgcgtct gcgtattatt ggcgatacca gtcgttttaa ctcgcgtttg
    37261 caagaacgta ttcgtaaatc tgaagcgcta acagccggaa ataccggtct gacgctgaat
    37321 attgcggcga actacggtgg acgttgggat atagtccagg gagtcaggca actggctgaa
    37381 aaggtgcagc aaggaaacct gcaaccagat cagatagatg aagagatgtt aaaccagcat
    37441 gtctgtatgc atgaactggc ccctgtagat ttagtaatta ggactggggg agagcatcgc
    37501 attagtaact ttttgctttg gcaaattgcc tatgccgaac tttactttac agatgttctc
    37561 tggcccgatt tcgatgaaca agactttgaa ggggcgttaa atgcctttgc taatcgagag
    37621 cgtcgtttcg gcggcaccga gcccggtgat gaaacagcct gatgggggtc gcttttgctg
    37681 aagtatcgcc tgatatctgc ttttgtgtta atacccgtcg tcatcgcggc gttgtttctg
    37741 ttgccgccgg tggggttcgc cattgtaacg ctggtggtct gcatgctggc agcgtgggaa
    37801 tggggacagc ttagcggttt taccactcgt tcgcagcgag tatggttggc ggtgttatgc
    37861 gggttattgt tggcgctgat gctttttctg ttgccggaat atcaccgaaa tattcatcaa
    37921 ccgctggttg aaatctcact ttgggcttcg ttgggttggt ggattgtcgc gctattgctg
    37981 gtgctgtttt acccaggttc cgcagcaatc tggcgtaact ctaaaacatt gcgccttatt
    38041 tttggcgtgc taaccattgt tcccttcttc tggggcatgc tggcgttacg ggcctggcac
    38101 tatgacgaga atcattacag tggcgcaata tggctgctct atgtcatgat cctggtatgg
    38161 ggcgctgact ccggcgcata tatgtttggc aaattgtttg gtaaacataa gctggcaccg
    38221 aaggtttctc cgggtaaaac ctggcaaggc tttataggtg gactcgctac tgcagcggta
    38281 atctcatggg gttatggcat gtgggcgaat ctcgacgtcg ctcccgtcac cttactcatt
    38341 tgctctattg tcgcagcgtt agcctcagtg ctcggcgatc tgaccgagag tatgtttaag
    38401 cgtgaagcag gaattaagga cagcggtcat ttaattccag gacacggtgg tattttagat
    38461 cgtattgata gcctgacggc tgcggtaccg gtctttgctt gcttgttgtt actggtattc
    38521 aggacgcttt aacggaaggt aatatgctga gttttctctg ggatttggct tcgttcatcg
    38581 ttgcactggg tgtacttatc accgtgcatg aatttggtca tttctgggtt gcccggcgtt
    38641 gtggtgttcg cgttgagcgt ttctcaatag ggtttggtaa ggcgctctgg cggcgaactg
    38701 ataagctcgg caccgaatat gttatcgccc tgatcccgtt gggcggttat gtcaaaatgc
    38761 tggatgagcg cgcagaaccg gtcgttccgg aactccgcca ccatgccttc aataataaat
    38821 ctgtcggcca acgagcggcg attattgccg caggtccggt tgcaaacttc atttttgcta
    38881 tctttgccta ctggctggtt tttattattg gtgtgcctgg cgtacgtccg gtggttggcg
    38941 aaatagcagc caattcgata gctgcggaag cacaaattgc accaggtacg gaactaaaag
    39001 ccgtagatgg tatcgaaacg cctgattggg atgccgtgcg tttgcagttg gtcgataaaa
    39061 ttggcgatga aagcaccacc attacagtag cgccatttga cagcgaccaa cggcgggatg
    39121 taaagctcga tttacgtcac tgggcgtttg agcctgataa agaagatccg gtatcttcgc
    39181 tggggattcg tcctcgtggg ccgcaaattg aacctgtact ggaaaatgtg cagccaaact
    39241 cggcggcaag caaggcaggt ttgcaagcag gcgacaggat cgttaaagtc gatggtcagc
    39301 ccttaacgca gtgggtgacc tttgtgatgc ttgtccggga taacccgggt aaatccttag
    39361 cgttagaaat cgaaaggcag gggagtccct tgtctttgac attaatcccg gagagtaaac
    39421 cgggtaatgg taaagcgatt ggttttgtcg gtattgagcc gaaagtcatt cctttgccag
    39481 atgagtataa agttgtacgc cagtatgggc cgttcaacgc catcgtcgaa gccacggaca
    39541 aaacgtggca gctgatgaag ctgacggtca gtatgctggg aaaattgatc accggtgatg
    39601 tgaaactgaa caacctcagt gggccgatct ctatcgccaa gggggctggg atgacagcgg
    39661 aactcggggt agtttattac ctgccgtttc ttgcgcttat tagcgtgaac ttagggataa
    39721 ttaacctgtt tccgttgccc gtacttgacg gggggcatct gctgttcctt gcgatcgaaa
    39781 agatcaaggg cggaccggta tccgagcggg ttcaagactt ttgttatcgc attggctcga
    39841 ttctgctggt gctgttaatg gggcttgcac ttttcaatga tttctctcgg ttatgagagt
    39901 tagttaggaa gaacgcataa taacgatggc gatgaaaaag ttgctcatag cgtcgctgct
    39961 gtttagcagc gccaccgtat acggtgctga agggttcgta gtgaaagata ttcatttcga
    40021 aggccttcag cgtgtcgccg ttggtgcggc cctcctcagt atgccggtgc gtacaggcga
    40081 cacggttaat gatgaagata tcagtaatac cattcgcgct ctgtttgcta ccggcaactt
    40141 tgaggatgtt cgcgtccttc gtgatggtga tacccttctg gttcaggtaa aagaacgtcc
    40201 gaccattgcc agcattactt tctccggtaa caaatcggtg aaagatgaca tgctgaagca
    40261 aaacctcgag gcttctggtg tgcgtgtggg cgaatccctc gatcgcacca ccattgccga
    40321 tatcgagaaa ggtctggaag acttctacta cagcgtcggt aaatatagcg ccagcgttaa
    40381 agctgtcgtg accccgctgc cgcgcaaccg tgttgaccta aaactggtgt tccaggaagg
    40441 tgtgtcagct gaaatccagc aaattaacat tgttggtaac catgctttca ccaccgacga
    40501 actgatctct catttccaac tgcgtgacga agtgccgtgg tggaatgtgg taggcgatcg
    40561 taaataccag aaacagaaac tggcgggcga tcttgaaacc ctgcgcagct actatctgga
    40621 ccgcggttat gcccgtttca acatcgactc tacccaggtc agtctgacgc cagataaaaa
    40681 aggtatttac gtcacggtga acatcaccga aggcgatcag tacaagcttt ctggcgttga
    40741 agtgagcggc aaccttgccg ggcactccgc tgaaattgag caactgacta agatcgagcc
    40801 gggcgagctg tataacggca ctaaagtgac caaaatggaa gatgacatta aaaagcttct
    40861 cggtcgctat ggttatgcct atccgcgcgt acagtcgatg cctgaaatta acgatgccga
    40921 caaaaccgtt aaattacgcg tgaacgttga tgcgggtaac cgtttctacg tgcgtaagat
    40981 ccgttttgaa ggtaacgata cctcgaaaga tgccgtcctg cgtcgcgaaa tgcgtcagat
    41041 ggaaggtgca tggttgggga gcgatctggt cgatcagggt aaggagcgtc tgaatcgtct
    41101 gggcttcttt gaaactgtcg ataccgatac ccaacgtgtt ccgggtagcc cggaccaggt
    41161 tgatgtagtc tacaaggtga aagaacgtaa cactggtagc ttcaacttcg ggattggtta
    41221 cggtactgaa agtggcgtga gcttccaggc tggcgtgcaa caggataact ggttaggtac
    41281 aggttatgct gttggtatca acgggaccaa aaacgattac cagacctatg ctgaactgtc
    41341 ggtaaccaac ccgtacttca ccgtagatgg cgtaagcctc ggtggtcgtc tcttctataa
    41401 tgacttccag gcagatgacg ccgacctgtc cgactatacc aacaagagtt atggtacaga
    41461 cgtgacgttg ggcttcccga ttaacgaata taactcgctg cgtgcaggtc tgggttatgt
    41521 acataactcc ctgtccaaca tgcagcctca ggttgcgatg tggcgttatc tgtactctat
    41581 gggtgaacat ccgagcacct ctgatcagga taacagcttc aaaacggacg acttcacgtt
    41641 caactatggt tggacctata acaagcttga ccgtggttac ttcccgacag atggttcacg
    41701 tgtcaacctg accggtaaag tgaccattcc tggatcggat aacgaatact acaaagtgac
    41761 gttagacacg gcgacttatg tgccgatcga tgacgatcac aaatgggttg ttctgggtcg
    41821 tacccgctgg ggttatggtg atggtttagg cggcaaagag atgccgttct acgagaactt
    41881 ctatgccggt ggttccagca ccgtgcgtgg cttccagtcc aataccattg gtccgaaagc
    41941 agtttacttc ccgcatcagg ccagtaatta tgatccggac tatgattacg aatgtgcgac
    42001 tcaggacggc gcgaaagacc tgtgtaaatc ggatgatgct gtaggcggta acgccatggc
    42061 ggttgccagc ctcgagttca tcaccccgac gccgtttatt agcgataagt atgctaactc
    42121 ggttcgtact tcattcttct gggatatggg taccgtttgg gatacaaact gggattccag
    42181 ccaatattct ggatatccgg actatagtga tccaagcaat atccgtatgt ctgcgggtat
    42241 cgcattacaa tggatgtccc cattggggcc gttggtgttc tcctacgccc agccgttcaa
    42301 aaagtacgat ggagacaagg cagaacagtt ccagtttaac atcggtaaaa cctggtaagt
    42361 gttctccaca aaggaatgta gtggtagtgt agcgatgact ttaggcgatc aatataagat
    42421 cgccgggcca cgcaaagaac tgcaccctcc ggtgcaaatg ggatggtaag gagtttattg
    42481 tgaaaaagtg gttattagct gcaggtcttg gtttagcact ggcaacttct gctcaggcgg
    42541 ctgacaaaat tgcaatcgtc aatatgggca gcctgttcca gcaggtagcg cagaaaaccg
    42601 gtgtttctaa cacgctggaa aatgagttca aaggccgtgc cagcgaactg cagcgtatgg
    42661 aaaccgatct gcaggctaaa atgaaaaaac tgcagtccat gaaagcgggc agcgatcgca
    42721 ccaaactgga aaaagacgtg atggctcagc gccagacttt tgctcagaaa gcgcaggctt
    42781 ttgagcagga tcgcgcacgt cgttccaacg aagaacgcgg caaactggtt actcgtatcc
    42841 agactgctgt gaaatccgtt gccaacagcc aggatatcga tctggttgtt gatgcaaacg
    42901 ccgttgctta caacagcagc gatgtaaaag acatcactgc cgacgtactg aaacaggtta
    42961 aataagtaat gccttcaatt cgactggctg atttagcgca gcagttggat gcagaactac
    43021 acggtgatgg cgatatcgtc atcaccggcg ttgcgtccat gcaatctgca caaacaggtc
    43081 acattacgtt catggttaac ccaaaatacc gtgagcattt aggcttgtgc caggcgtccg
    43141 cggttgtcat gacccaggac gatcttcctt tcgcgaaaag tgccgcgctg gtagtgaaga
    43201 atccctacct gacttacgcg cgcatggcgc aaattttaga taccacgccg cagcccgcgc
    43261 agaacattgc acccagtgcg gtgatcgacg cgacggcgaa gctgggtaac aacgtatcga
    43321 ttggcgctaa cgcggtgatt gagtccggcg ttgaactggg cgataacgtg attatcggtg
    43381 ccggttgctt cgtaggtaaa aacagcaaaa tcggtgcagg ttcgcgtctc tgggcgaacg
    43441 taaccattta ccatgagatc cagatcggtc agaattgcct gatccagtcc ggaacagtgg
    43501 taggcgcaga cggctttggt tatgccaacg atcgtggtaa ctgggtgaag atcccacaga
    43561 ttggtcgcgt aattattggc gatcgcgtgg agatcggtgc ctgcacaacc atcgatcgcg
    43621 gcgcgctgga tgacactgtt attggcaatg gcgtgatcat tgataaccag tgccagattg
    43681 cacataacgt cgtgattggc gacaatacgg cggttgccgg tggcgtcatt atggcgggca
    43741 gcctgaaaat tggtcgttac tgcatgatcg gcggagccag cgtaatcaac gggcatatgg
    43801 aaatatgcga caaagtgacg gttacgggca tgggtatggt gatgcgtccc atcactgaac
    43861 caggcgtcta ttcctcaggc attccgctgc aacccaacaa agtctggcgc aaaaccgctg
    43921 cactggtgat gaacattgat gacatgagca agcgtctgaa atcgcttgag cgcaaggtta
    43981 atcaacaaga ctaacgttcc atcttttgtt cgccaaactt tacggcctgt ctcattctta
    44041 cgattgcggc aggccgtgtt attattgtcg tttcttatat tttgacagga agagtatctt
    44101 gactactaac actcatactc tgcagattga agagatttta gaacttctgc cgcaccgttt
    44161 cccgttctta ctggtggatc gcgtgctgga ttttgaagaa ggtcgttttc tgcgcgcagt
    44221 aaaaaatgtc tctgtcaatg agccattctt ccagggccat ttccctggaa aaccgatttt
    44281 cccgggtgtg ctgattctgg aagcaatggc acaggcaaca ggtattctgg cgtttaaaag
    44341 cgtaggaaaa ctggaaccgg gtgagctgta ctacttcgct ggtattgacg aagcgcgctt
    44401 caagcgcccg gtcgtgcctg gcgatcaaat gatcatggaa gtcactttcg aaaaaacgcg
    44461 ccgcggcctg acccgtttta aaggggttgc tctggtcgat ggtaaagtag tttgcgaagc
    44521 aacgatgatg tgtgctcgta gccgggaggc ctgatacgtg attgataaat ccgcctttgt
    44581 gcatccaacc gccattgtgg aagagggcgc gtcaattggc gcgaacgcac acattggtcc
    44641 tttttgtatc gttggacccc atgtcgaaat tggtgagggt accgtactga aatctcacgt
    44701 tgtcgtgaat ggtcatacta aaattggccg cgataatgag atttatcagt ttgcctccat
    44761 cggcgaagtt aaccaggatt tgaaatatgc tggcgaaccg acccgtgtgg aaatcggcga
    44821 tcgtaaccgc attcgcgaaa gcgtcaccat tcatcgtggc acagtccagg gcggtggatt
    44881 gacgaaggtg ggcagcgaca acttactgat gatcaacgcg cacattgcgc acgattgtac
    44941 ggtaggtaac cgctgtattc tcgccaacaa cgcaacgctg gcgggtcacg tatcggttga
    45001 cgacttcgcg atcatcggcg gcatgaccgc agtccatcag ttctgcatca ttggtgcgca
    45061 tgtgatggtt ggcggctgct ccggtgtggc gcaggacgtc cctccttatg ttattgcgca
    45121 gggtaaccac gcaacgccgt tcggtgtcaa tatcgaaggg ctgaagcgcc gcggattcag
    45181 ccgtgaggcg attaccgcta tccgcaatgc gtataagcta atttatcgta gcggtaaaac
    45241 gctcgatgaa gtgaaaccgg aaattgctga actggcggaa acatatccgg aagtgaaagc
    45301 ctttaccgat ttctttgcac gctcaacgcg cggtctgatt cgttaatgac tgaccagcgt
    45361 ccattaacga ttgccctggt cgccggagaa acctccggcg atatcctggg ggccggttta
    45421 atccgcgctc tgaaagaacg tgtgcccaac gcccgctttg ttggtgttgc cgggccacga
    45481 atgcaggctg aaggctgcga agcctggtac gaaatggaag aactggcggt gatgggcatt
    45541 gttgaagtgc tcggtcgtct gcgtcgctta ctgcatattc gtgccgatct gacaaagcgt
    45601 tttggcgaac tgaagccaga tgtttttgtt ggtattgatg cgcccgactt caatattact
    45661 cttgaaggta acctcaaaaa gcagggtatc aaaaccattc attacgtcag tccatccgtc
    45721 tgggcgtggc gacagaaacg cgttttcaaa ataggcagag ccaccgatct ggtgctcgca
    45781 tttctgcctt tcgaaaaagc gttttatgac aaatacaatg taccgtgccg ctttatcggt
    45841 cataccatgg ctgatgccat gccgttagat ccagataaaa atggtgcccg tgatgtgctg
    45901 gggatccctt acgatgccca ctgtctggca ttgttgccgg gcagccgtgg tgcagaagtc
    45961 gaaatgctta gtgccgattt cttgaaaact gcccagcttt tgcgccagac atatccggat
    46021 ctcgaaatcg tggtgccgct ggtgaatgcc aaacgccgcg agcagtttga acgcatcaaa
    46081 gctgaagtcg cgccagacct gtcggttcat ttgctggatg gaatgggccg tgaggcgatg
    46141 gtcgccagcg atgcggcact actggcgtcg gggacggcag ccctggagtg tatgctggcg
    46201 aaatgcccga tggtggtggg atatcgcatg aagcctttta ccttctggtt ggcgaagcgg
    46261 ctggtgaaaa ctgattatgt ctcgctgcca aatctgctgg cgggcagaga gttagtcaaa
    46321 gagttattgc aggaagagtg tgagccgcaa aaactggctg cggcgctgtt accgctgttg
    46381 gcgaacggga aaaccagcca cgcgatgcac gataccttcc gtgaactgca tcagcagatc
    46441 cgctgcaatg ccgatgagca ggcggcacaa gccgttctgg agttagcaca atgatcgaat
    46501 ttgtttatcc gcacacgcag ctggttgcgg gtgtggatga agtcggacgc gggccgttag
    46561 ttggcgcggt cgtcaccgct gcggtgatcc ttgacccggc gcgcccgatt gccgggctga
    46621 atgattccaa aaagctgagc gaaaaacgcc gtctggcgct ctatgaagag atcaaagaga
    46681 aagcgttgag ctggagtctg ggccgcgcgg aaccccacga aatcgacgag ctgaacattc
    46741 ttcatgcgac catgctggcg atgcagcgtg ccgtcgctgg gctgcatatt gcgccggaat
    46801 atgtgttgat tgatggtaac cgctgcccga aattaccgat gcctgcgatg gctgtggtga
    46861 aaggggatag ccgcgtacca gaaatcagtg ccgcgtctat cctggcgaaa gtgacgcgtg
    46921 acgccgaaat ggcggcgctg gatattgttt tcccgcaata tggttttgcc caacataaag
    46981 ggtatccaac cgcttttcat ctggaaaaac tggctgaata tggcgcgact gaacatcatc
    47041 gtcgcagctt tgggcctgtc aaacgcgcac tgggacttgc gtcctgattc ttgtgtcgag
    47101 attaagtaaa ccggaatctg aagatgtctg aaccacgttt cgtacacctg cgggtgcaca
    47161 gcgactactc gatgatcgat ggcttggcca aaaccgcacc gctggtaaaa aaggcggcgg
    47221 cgttgggtat gccagcactg gcgatcaccg atttcaccaa cctttgcggt ctggtgaagt
    47281 tctacggagc gggacatggc gcagggatta agcctatcgt cggggcagat tttaatgtcc
    47341 agtgcgacct gctgggtgat gagttaaccc acctgacggt actggcggcg aacaataccg
    47401 gctatcagaa tctggcgttg ctgatctcaa aagcgtatca gcgcgggtac ggtgccgccg
    47461 ggccgatcat cgatcgcgac tggcttatcg aattaaacga agggttgatc cttctttccg
    47521 gcgggcgcat gggcgacgtc ggacgcagtc ttttgcgtgg taacagcgcg ctggtagatg
    47581 agtgtgtcgc gttttatgaa gaacacttcc cggatcgcta ttttctcgag ctgatccgca
    47641 ccggcaggcc ggatgaagaa agctatctgc acgcggcggt ggaactggcg gaagcgcgcg
    47701 gtttgcccgt cgtggcgact aacgatgtgc gctttatcga cagcagcgac tttgacgcac
    47761 acgaaatccg cgtcgcgatc cacgacggct ttaccctcga cgatcctaaa cgcccgcgta
    47821 actattcgcc gcagcaatat atgcgtagcg aagaggagat gtgtgagctg tttgccgaca
    47881 tcccagaagc ccttgccaac accgttgaga tcgccaaacg ctgtaacgta actgtgcgtc
    47941 ttggtgaata cttcctgccg cagttcccga ccggggacat gagcaccgaa gattatctgg
    48001 tcaagcgtgc aaaagaaggc ctggaagagc gtctggcatt tttattccct gacgaggaag
    48061 aacgtcttaa acgccgcccg gaatatgacg aacgtctgga gactgaactt caggttatca
    48121 accagatggg cttcccgggc tacttcctca tcgttatgga atttatccag tggtcgaaag
    48181 ataacggcgt accggtaggg ccaggccgtg gctccggtgc gggttcactg gtggcctacg
    48241 cgctgaaaat caccgacctc gatccgctgg aatttgacct gctgttcgaa cgtttcctta
    48301 acccggaacg tgtctccatg cctgacttcg acgttgactt ctgtatggag aaacgcgatc
    48361 aggttatcga acacgtggcg gacatgtacg gtcgtgatgc ggtatcgcag attatcacct
    48421 tcggtacgat ggcggcgaaa gcggtgatcc gcgacgtagg ccgcgtgctg gggcatccgt
    48481 acggctttgt cgaccgtatc tcgaaactga tcccgcccga tccggggatg acgctggcga
    48541 aagcgtttga agccgagccg cagctgccgg aaatctacga agcggatgaa gaagttaagg
    48601 cgctgatcga catggcgcgc aaactggaag gggtcacccg taacgccggt aagcacgccg
    48661 gtggggtggt tatcgcgccg accaaaatta ccgattttgc gccgctttat tgcgatgaag
    48721 agggcaaaca tccggtcacc cagtttgata aaagcgacgt tgaatacgcc ggactggtga
    48781 agttcgactt ccttggtttg cgtacgctca ccatcatcaa ctgggcgctg gagatgatca
    48841 acaagcggcg ggcgaagaat ggcgagccac cgctggatat cgccgcgatc ccgctggatg
    48901 acaagaaaag cttcgacatg ctgcaacgct cggaaaccac ggcggtattc cagcttgaat
    48961 cgcgcggcat gaaggacctg atcaagcgtc tgcaacctga ctgcttcgaa gatatgatcg
    49021 cactggtggc actgttccgc cctggtccgt tgcagtcagg gatggtggat aactttatcg
    49081 accgtaaaca tggtcgtgaa gagatctcct atccggacgt gcagtggcag catgaaagcc
    49141 tgaaatcggt actggagcca acctacggca ttatcttgta tcaggaacag gtcatgcaga
    49201 ttgcccaggt gctttctggt tataccctcg gtggcgcgga tatgctgcgc cgtgcgatgg
    49261 gtaagaaaaa gccggaagag atggctaagc agcgttctgt atttgctgaa ggtgcagaaa
    49321 agaacggaat caacgccgaa ctggcgatga aaatcttcga cctggtggag aaattcgcgg
    49381 gttacggatt taacaaatcg cactctgcgg cctatgcttt ggtgtcatat caaacgttat
    49441 ggctgaaagc gcactatccg gcggagttta tggcggcggt aatgaccgcc gatatggaca
    49501 acaccgagaa ggtggtgggc ctggtggatg agtgctggcg gatggggctg aaaatcctgc
    49561 caccagatat aaactccggt ctttaccatt tccacgtcaa cgacgatggc gaaatcgtgt
    49621 atggtattgg cgccatcaaa ggggtaggtg aaggtccgat tgaggccatc atcgaagccc
    49681 gtaataaagg cggctacttc cgcgaactgt ttgatctctg cgcccgtacc gacaccaaaa
    49741 agttaaaccg gcgggtgctg gaaaaactga tcatgtccgg ggcgtttgac cgtcttgggc
    49801 cacaccgcgc ggcgctgatg aactcgctgg gtgatgcgtt aaaagcggca gatcaacacg
    49861 cgaaagcgga agctatcggt caggccgata tgttcggcgt gctggccgaa gagccggaac
    49921 aaattgaaca atcctacgcc agctgccaac cgtggccgga gcaggtggta ttagatgggg
    49981 aacgtgaaac gttaggcctg tacctgaccg gacaccctat caaccagtat ttaaaagaga
    50041 ttgagcgtta tgtcggaggc gtaaggctga aagacatgca cccgacagaa cgtggtaaag
    50101 tcatcacggc tgcggggctc gttgttgccg cgcgggttat ggtcaccaag cgcggcaatc
    50161 gtatcggtat ctgcacgctg gatgaccgtt ccgggcggct ggaagtgatg ttgtttactg
    50221 acgccctgga taaataccag caattgctgg aaaaagaccg catacttatc gtcagcggac
    50281 aggtcagctt tgatgacttc agcggtgggc ttaaaatgac cgctcgcgaa gtgatggata
    50341 ttgacgaagc ccgggaaaaa tatgctcgcg ggcttgctat ctcgctgacg gacaggcaaa
    50401 ttgatgacca gcttttaaac cgactccgtc agtctctgga accccaccgc tctgggacaa
    50461 ttccagtaca tctctactat cagagggcgg atgcacgcgc gcggttgcgt tttggcgcga
    50521 cgtggcgtgt ctctccgagc gatcgtttat taaacgatct ccgtggcctc attggttcgg
    50581 agcaggtgga actggagttt gactaataca ggaatactat gagtctgaat ttccttgatt
    50641 ttgaacagcc gattgcagag ctggaagcga aaatcgattc tctgactgcg gttagccgtc
    50701 aggatgagaa actggatatt aacatcgatg aagaagtgca tcgtctgcgt gaaaaaagcg
    50761 tagaactgac gcgtaaaatc ttcgccgatc tcggtgcatg gcagattgcg caactggcac
    50821 gccatccgca gcgtccttat accctggatt acgttcgcct ggcatttgat gaatttgacg
    50881 aactggctgg cgaccgtgcg tatgcagacg ataaagctat cgtcggtggt atcgcccgtc
    50941 tcgatggtcg tccggtgatg atcattggtc atcaaaaagg tcgtgaaacc aaagaaaaaa
    51001 ttcgccgtaa ctttggtatg ccagcgccag aaggttaccg caaagcactg cgtctgatgc
    51061 aaatggctga acgctttaag atgcctatca tcacctttat cgacaccccg ggggcttatc
    51121 ctggcgtggg cgcagaagag cgtggtcagt ctgaagccat tgcacgcaac ctgcgtgaaa
    51181 tgtctcgcct tggcgtaccg gtagtttgta cggttatcgg tgaaggtggt tctggcggtg
    51241 cgctggcgat tggcgtgggc gataaagtga atatgctgca atacagcacc tattccgtta
    51301 tctcgccgga aggttgtgcg tccattctgt ggaagagcgc cgacaaagcg ccgctggcgg
    51361 ctgaagcgat gggtatcatc gctccgcgtc tgaaagagct gaaactgatc gactccatca
    51421 tcccggaacc gctgggcggc gctcaccgta acccggaagc gatggcggca tcgttgaaag
    51481 cgcaactgct ggcggatctg gccgatctcg atgtgttaag cactgaagat ttaaaaaatc
    51541 gtcgttatca gcgcctgatg agctacggtt acgcgtaatt cgcaaaagtt ctgaaaaagg
    51601 gtcacttcgg tggccctttt ttatcgccac ggtttgagca ggctatgatt aaggaaggat
    51661 tttccaggag gaacacatga acatcattgc cattatggga ccgcatggcg tcttttataa
    51721 agatgagccc atcaaagaac tggagtcggc gctggtggcg caaggctttc agattatctg
    51781 gccacaaaac agcgttgatt tgctgaaatt tatcgaacat aaccctcgaa tttgcggcgt
    51841 gatttttgac tgggatgagt acagtctcga tttatgcagc gatatcaatc aacttaatga
    51901 atatctcccg ctttatgcct tcatcaacac ccactcgacg atggatgtca gcgtgcagga
    51961 tatgcggatg gcgctctggt tttttgaata tgcgctgggg caggcggaag atatcgccat
    52021 tcgtatgcgt cagtacacca acgaatatct tgataacatt acgccgccgt tcacgaaagc
    52081 cttgtttacc tacgtcaaag agcggaagta caccttttgt acgccggggc atatgggcgg
    52141 caccgcatat caaaaaagcc cggttggctg tctgttttat gattttttcg gcgggaatac
    52201 ccttaaggct gatgtctcta tttcggtcac cgagcttggt tcgttgcttg accacaccgg
    52261 gccacacctg gaagcggaag agtacatcgc gcggactttt ggcgcggaac agagttatat
    52321 cgttaccaac ggaatatcga cgtcgaacaa aattgtgggt atgtacgccg cgccatccgg
    52381 cagtacgctg ttgatcgacc gcaactgcca taaatcactg gcgcatctgt tgatgatgaa
    52441 cgatgtagtg ccagtctggc tgaaaccgac gcgtaatgcg ttggggattc tgggggggat
    52501 cccgcgccgt gaatttactc gcgacagcat cgaagagaaa gtcgctgcca ctacgcaagc
    52561 acaatggccg gttcatgcgg tgatcaccaa ctccacctat gatggtttgc tctacaacac
    52621 cgactggatc aaacagacgc tggatgtccc gtcgatccac ttcgattccg cctgggtgcc
    52681 gtatactcat tttcatccaa tctaccaggg taaaagtggt atgagcggcg agcgtgttgc
    52741 gggaaaagtt ttcttcgaaa cgcaatcgac ccacaaaatg ctggcggcgt tatcgcaggc
    52801 ttcactgatc cacattaaag gcgagtatga cgaagaggcg tttaacgaag cctttatgat
    52861 gcataccacc acctcgccca gttatcccat tgttgcttcg gttaagacgg cggcggcgat
    52921 gctgcgtggt aatccgggca aacggctgat taaccgttcg gtggagcggg cgctgcattt
    52981 tcgcaaagag gtccagcggc tgcgggaaga gtctgacggt tggtttttcg atatctggca
    53041 accgccgcag gtggatgaag ccgaatgcag gcccgttgcg cctggcgaac aatggcacgg
    53101 ctttaacgat gcggatgccg atcatatgtt tctcgatccg gttaaagtca ctattttgac
    53161 accggggatg gacgaacagg gcaatatgag tgaggagggg atcccggcgg cgctggtagc
    53221 aaaattcctc gacgaacgtg ggatcgtagt agagaaaacc ggcccttata acctgctgtt
    53281 tctctttagt attggcatcg ataaaaccaa agcaatggga ttattgcgtg ggttgacgga
    53341 atttaagcgc tcttacgatc tcaacctgcg gatcaaaaat atgctgcccg atctctatgc
    53401 tgaagatccc gatttctacc gcaatatgcg tattcaggat ctggcgcaag ggatccataa
    53461 gctgattcgt aaacacgatc tttccggttt gatgttgcgg gcattcgata ctttgccgga
    53521 aatgatcatg acgccacatc aggcatggca gagacagatt aaaggtgaag tagaaaccat
    53581 tgcgctggaa caactggtcg gtagagtatc ggcaaatatg atcctgcctt atccaccggg
    53641 cgtaccgctg ttgatgccag gagaaatgct gaccaaagag agccgcacgg tactcgattt
    53701 tctactgatg ctttgttccg tagggcaaca ttaccccggt tttgaaacgg atattcacgg
    53761 cgcgaaacag gacgaagacg gcgtttaccg cgtacgagtc ctaaaaatgg cgggataact
    53821 tgccagagcg gcttccgggc gagtaacgtg ctgttaacaa ataaaggaga cgttatgctg
    53881 ggtttaaaac aggttcacca tattgcgatt attgcgacgg attatgcggt gagcaaagct
    53941 ttctactgcg atattcttgg tttcacgctg caaagcgaag tctatcgcga agcgcgcgac
    54001 tcgtggaaag gggatttggc gcttaatggg caatatgtga ttgagctttt ctcttttccg
    54061 ttcccgccgg agcgccccag ccgaccggaa gcttgcggcc tgcgtcatct ggcttttagc
    54121 gttgatgaca tcgatgcggc agtggcgcac cttgaaagcc ataacgtgaa gtgtgaagcc
    54181 atccgtgtcg atccatacac gcaaaaacgc ttcaccttct ttaacgatcc ggacgggctg
    54241 ccgttggaac tgtatgagca gtaaggcttg tcatcgccgc atttgcccgg taacgtgccg
    54301 ggcattgcta ctgtaaaatc gcaccatcat gacactcacg ctcaatagac aacttctcac
    54361 ctcacgccag attctggtgg cctttagcgg cgggcttgac tccaccgttc tgctgcatca
    54421 gttggtgcag tggcggacgg aaaatccggg cgtcactctg cgcgctattc atgtgcatca
    54481 cggtttaagt gccaatgccg atgcctgggt tacgcattgc gaaaacgtct gccaacagtg
    54541 gcaggtgccg ctggtggtcg aacgcgtaca acttgcgcaa gaggggctgg ggattgaggc
    54601 ccaggcgcgg caggcacgtt atcaggcatt tgcccgtacc ctattgcccg gcgaagtgct
    54661 ggtcaccgcg caacatctcg acgatcaatg tgaaaccttt ctgctggcgc taaaacgcgg
    54721 tagcggccct gccgggcttt cggctatggc ggaagtttcg gagtttgctg gaacacggct
    54781 tatccgcccg ttgcttgccc gcacgcgggg ggaactggag cagtgggcat tggcgcatgg
    54841 tttacgctgg attgaagacg aaagtaatca ggacgactca tacgatcgta actttctgcg
    54901 cctgcgcgta gtgccgttat tgcagcagcg ttggccgcat tttgcggaag caacggcccg
    54961 cagcgccaca ctttgtgctg aacaagagag tctgttggat gaactgctgg cggatgattt
    55021 agcacactgc caaacgtcgc aggggacgct gcagattgcg ccaatgctgg cgatgagtga
    55081 tgcccgtcgc gcggcgatta ttcgccgctg gctggcaggg cagaatgcac cgatgccttc
    55141 ccgcgacgcg ctggtgcgca tctggcagga agtggcgctg gcgcgggaag atgcttcccc
    55201 ctgtttacgt ttgggcgcct ttgaaatccg ccgctatcag tcgcaactgt ggtggattaa
    55261 atccgtcacc gggcaaagcg aaaccattgt gctgtggcag acgtggctac aaccgctgga
    55321 attaccggcg gggctgggaa ccgtacagct tactgcggga ggcgatattc gccctccgcg
    55381 tgcagacgaa gcggtcagcg tgcgtttcaa agcgccagga ttgctgcata ttgtcgggcg
    55441 caacggcgga cgtaagctga agaaaatctg gcaagagtta ggcgtgccgc cgtggctacg
    55501 tgacaccacg ccactgctgt tttatggaga aacgctgatt gcggcggcag gggtatttgt
    55561 gacgcaagaa ggtgtggctg aaggtgagaa tggcgtaagt tttgtctggc agaaaacgct
    55621 tagttaagtg aaagccggat aagacgcatc aaacgtcgca tccggcgaaa gtcaatcagg
    55681 attcgcttac caccaccgta ccgatttcag ggtggctaaa gctggtaatt ttatccagac
    55741 gcagctcgcg ggtttcgcca gcggcctcga cgaccagata ctccacattt ttgcgggaga
    55801 ctaaatcact ggcttttgcc tgcaattttt cgccatcttt cagctcaagt gtcagcatta
    55861 aatgatgctg gcaggcgagc tcaagattat cgtaatcatc acaattaatt ggttgatacg
    55921 tatcattcat tgacataatc gctcaccagt aagtttgccg cagcgtatgc tgctttttcc
    55981 ctgacagcct cagaaagggc gtcgtcggca gccatttcat tcagcacttt caaaacgcag
    56041 cccagcgcgt ccggaacgta tcctaagtct ccgctggcga tttccgcgta ccgcttgcgt
    56101 attaactcac aatatttttc cacatgccct cctgtcagca ctctgactta accgtggatg
    56161 caagtctaag cctacgaaga taaactctgt ttcgcaaggt gactatacca cactcatttc
    56221 tgcaatatca gcgccgcaac tgcacgtatt ccgttacaat ggcctcctga ttcgaaagga
    56281 gttttcttat ggcgcttaaa gcgacaattt ataaagcgac ggttaatgtg gccgatctcg
    56341 accgcaacca gtttctcgat gcctctctga cgctggcgcg ccacccttca gaaacccagg
    56401 agcgtatgat gctgcgcttg ctggcgtggc tgaaatatgc tgatgaacgt ctgcaattta
    56461 cccgtggttt gtgtgccgat gatgagccgg aagcgtggct gcgtaacgat cacctgggca
    56521 ttgatttgtg gattgagctg gggctgccgg atgagcgacg aattaagaaa gcctgcaccc
    56581 aggccgcaga agtggcgctg tttacctata atagtcgggc ggcgcaaatc tggtggcagc
    56641 aaaatcagag caaatgtgtg cagtttgcca atctttccgt ctggtatctg gacgatgaac
    56701 aactggcgaa agtaagcgcc tttgccgatc gtaccatgac gctgcaggca acgattcagg
    56761 atggtgttat ctggttatcg gatgataaga ataatctgga agttaactta accgcctggc
    56821 aacaaccttc atgattgtga tttcccgaca tgttgctatt cccgatggtg agcttgagat
    56881 caccgccatt cgtgcgcagg gcgctggcgg gcagcatgtt aataagacct caacggctat
    56941 tcatctgcgt tttgacattc gggcgtccag cctgccagag tattacaaag agcgtctgct
    57001 cgccgccagc catcatttga tcagcagtga tggcgtgatt gtcattaagg cacaggaata
    57061 ccgcagtcag gaactgaacc gcgaagccgc tctggcccgg ctggtggcag tgattaaaga
    57121 tttaacaaaa gaacaaaaag cccgacgacc cacgcggccc acccgtgcat cgaaagagcg
    57181 caggctggca tcgaaagcac aaaaatcaag cgtgaaggcg atgcgcggca aagtacgcag
    57241 cggtcgggaa taaaaagaag gaatggatgg tgaaaaaagc gatagtgaca gcgatggctg
    57301 taatcagcct ctttactctg atgggatgta ataatcgggc cgaagtcgat acgctttctc
    57361 cggcgcaggc tgccgaactg aaaccgatgc cgcaaagttg gcgcggcgtg ctaccgtgtg
    57421 ccgattgcga aggaatcgaa atctctctgt tcctcgaaaa agacggaaca tgggtgatga
    57481 atgagcgtta tctcggtgct cgtgaagaac cttcctcctt cgcttcctac ggtacatggg
    57541 cgcgaaccgc tgacaagctg gtattaaccg acagcaaagg tgaaaagtca tattatcggg
    57601 cgaaaggcga tgcgctggag atgctcgatc gtgaaggcaa tccaattgaa tcgcagttca
    57661 actatacgct ggaaccggca caatccagtt tacctatgac gccgatgacc ctgcgaggca
    57721 tgtattttta tatggctgat gcggcgacct tcactgattg cgcgaccgga aaacgtttca
    57781 tggtagcgaa taacgcagag ctggagcgta gctacctggc tgcgcgcggt cacagtgaaa
    57841 aaccggtgtt actgtcagta gaaggtcact ttacgcttga ggctaatccg gatactggtg
    57901 cgccaaccaa agtattagcg cccgatacgg cgggtaaatt ttacccgaat aaggattgca
    57961 gtagtttggg gcagtaaacc tgcttaaggc agaaacaaac gcaaaattgc ctgatgcgct
    58021 gcgcttatca ggcctacagc atataatgca acattttgaa ttagcacgat tttgtaggcc
    58081 ggataaggcg tttacgccgc atccggcatt ttgtgcacga ggctggcttt atcactgagt
    58141 cagcccaaca aagcgacccg ccttgatata aatcccaggc ttcaggtgcc caacatactg
    58201 taactgcgtt tctggcttaa acgcagagac atcaccaata cgcatatgca tcagatcggc
    58261 agggctaatc cagcccgatt gcgccagtgt aagtggatgc ccggatttgg caaatgcatc
    58321 agtgacaaat tccgaacaaa accacgactt tttgtctcct tcgcacacac tgcttaactg
    58381 cgctttcgcc aggtcgctga cgcactgttg gcgaaaatcc tcggagaacg gattcagtga
    58441 gcacatctgg cgagtcacca taaagggaat aaattcgaca atgccgcgat agttataacc
    58501 gctatctttg attttattgg caaacgcggt gatatctgtg gcttgttgcg gggtaagatc
    58561 cgggactcgt aagacgaaaa gcttatcact atgcttcatc gcttttttaa gggaaacaat
    58621 ctggacgcca gcgcctgtcg cttctgcaac gttattatca cccagaaaga ttgcaacgtg
    58681 actcacagag gaagtgctga agacgcggat tccaaatgag gttaccccaa ggcttgagga
    58741 gaacagcaaa tcgccgggtt tgagatcagg tgccgttatt tcttttattg attgttcggt
    58801 gaaagagctt tgatgctgga atttgacagc ccatgttttt gcctcggcat ctactgctgt
    58861 ggctgatggg tctggctggc tgatatcaac ggtacaagcc gaaagtaaaa gaaagctggg
    58921 gagaagcagg cggcagtacg cctttggttt atccatttta tacaatccat gtaaaaaagg
    58981 gccctgaaat tcaggaccct ttctggcatc agcctttaat ctgtttcacc agatattcga
    59041 cgatgtcacc agtcttaatt aactgtttct cgccgttgcg acgatattta tattcgatat
    59101 cgtcgttgtc gaggttacgg tcgcccagca caatagtgtg cggaataccg atcagttcca
    59161 tatcagcaaa catcacgccc ggacgctctt tgcggtcatc cagcagcact tcgatacctt
    59221 gtgcacgcag ttcgctgtac agtttctcag caagctcttg tacgcggaag gatttgtgca
    59281 tgttcatcgg cagaatcgcc acctggaacg gcgcgatagc gtcaggccat acgatgccgc
    59341 gttcgtcgta gttctgctca atcgccgcag ctaccacacg cgttaccccg ataccgtagc
    59401 aacccatcgt caggatttgg ttacggccat cttcaccctg tacggaggct ttcagtgctt
    59461 cggagtactt ggtacccagc tggaagatgt gaccaacttc gataccacgt ttgatcagca
    59521 gcgtaccctg gccatccggg cttggatcgc cagccaccac gttacggata tctgcaactt
    59581 ccggggtagc gacatcgcga tcccagttga taccgaagta gtgtttacca tcgatgttag
    59641 caccagcagc gaaatcactc atcgccgcaa cggtacggtc aatcaccacc ggaatcggca
    59701 tgtttaccgg acccagtgaa cccggaccgg ctttaaccac agcacgaatt tcttcttcgg
    59761 tcgcgaaagt cagcgggctt gcaacctgcg gcagtttttc tgctttaact tcgttcagct
    59821 cgtgatcacc gcgcaccagc agcgcaacca gcgggaagct actgccttca accgctttaa
    59881 ccagcagagt cttaaccgtt ttctcaatcg gcagattgaa ctgttcaacc agttccgcga
    59941 tggttttcgc gttcggcgta tcaaccagcg tcatttcctg ggtagcagca gcgcgcggtt
    60001 ctttcggcgc gatagcttct gccagttcaa tgttcgctgc atagtcagag gtgtcggaga
    60061 agaccacatc gtcttcaccg ctctgcgcca gcacctggaa ttcgtgagag gcgctgccgc
    60121 cgatagaacc ggtgtcggcc tgtacggcgc ggaaatccag ccccatgcgg ctgaagattt
    60181 tgctgtaggc cgcatacatt gcatcgtagg tttcctgcag ggattcctga gaagtatgga
    60241 aagagtaagc atctttcatc aggaattcgc gggaacgcat gacgccgaaa cgcggacgca
    60301 cttcgtcgcg gaacttggtc tggatctgat agaagttcag cggcagctgt ttgtaagagc
    60361 taagctcgtt acgaatcagg tcagtgataa cttcttcatg agttgggccg agtacgaacg
    60421 gacgctcgcc acggtcaaca aaacgcagca gttccggacc gtactgttcc caacgaccac
    60481 tctcttgcca caaatcggct ggctgaacca ccggcatcga cacctcgatc gcaccggcgt
    60541 tgttcatctc ttcacgcacg atgttttcga cttttttcag aacgcgcacg ccggtcggca
    60601 gccaggtata taacccggag gccagcttgc ggatcatccc ggcgcgcagc atcagctgat
    60661 ggctgatcac ctcggcgtcg gcaggtgtct ccttgagagt ggagagcagg tattggctag
    60721 tacgcatgtt gttacggttc cagttggaag gtagaacagg ctcaaggcga gcctgggaca
    60781 aaaaaagtga tttagtttac cagtgcaaaa gaaatgtcaa aagagaaggg cgtgaattta
    60841 acgcggttcc agcgcaaaga cttcaaaacc tgcgtcggtg acgcgccagc gaacgttaaa
    60901 atcatgtagc cagacggcat aggttttgcc cgtttcctca cctttacgat aggccgggcg
    60961 cgggtcctgc gccagtactt cgcggataaa cagcgttaac tgcggataac gcttctccag
    61021 cgtcaaaagc tgcttttcga cctctgcggt aaaactcacc gccatctctg cagctggcgc
    61081 actttgcgca tagctggcac tggcatcggg aagcgattcg gcaaagggga gatacggttt
    61141 gatatccact accggcgtac catcgaccag atccagactg ccgagcttca gaatcacgct
    61201 gcctttatgg caaacaacct ctttcagctc taccagcgac atgccaattg ggttagggcg
    61261 gaaagtagag cgtgttgcga aaacccccat tctggcgtta ccgccgaggc gcggtggacg
    61321 cacagtcgga cgccagccgc cttccatcgt ttgatgaaag acgaaaagga tccataaatg
    61381 gctgaatgct tccaggccgc gaacggcgtc ggcctggttg taggaagcta tgagatgcag
    61441 ttcgccgttg gcgcttttta ccagacctgg ctggcgcgga acggcgaatt tttctttata
    61501 gggcgagcga ataacgccta tttgctcgaa ctggaaactg ctcatttcgc cgtaatgtta
    61561 agcgcagagc cgatacatac agcctgacga tagcagcccg gcgtaccgct ggtgacttcg
    61621 cagctatgca gtaatacagc attggctttc attttagagg cattgatttg catctgctta
    61681 cgtgcggttg gaatgctcgg cggagagtcc tgattagagg cctggcaaga gtcgccactg
    61741 acttcaccga gatcgcggaa cggtttgccg actaattctt ctgcattggt ataaattcgg
    61801 accggcgtgg cgcgcggcgc tttcggtttt gcaggctccg ctttcggctg gggtgcagtg
    61861 ctttgaacgg gttcgacagg ggatctgctt aacatggaac agccgcttag catgagtgct
    61921 actaaacaga tcggtaaagc acgcatagta tttcctcaat gtatgatcaa aacgtcaata
    61981 ttgaatcagg agcttgtaaa aatgacaaga cgggcaagcg cccgtcctga atgatattac
    62041 aaattgtgga aacagcctaa aaattaccag cctttaacag cgccgccgtt aaacactttg
    62101 tttgctgctt cgtaaacttc atcagactga taagcctgga cgaatttctt cacgttctcg
    62161 gcgtctttgt tatcttcacg cgttacgatc aggtttacgt atggggagtc tttatcttcg
    62221 acaaagatac cgtctttcgc cggagtcagg ccaatctggc tggcataggt ggtattgata
    62281 actgccagag cgatttgcgc gtcgtccaga gaacgcggca gttgcggtgc ttccagttca
    62341 acaattttca gattttttgg gttctcaaca acatcaagaa cggtcggcag caggccaacg
    62401 ccatctttca gtttgatcaa gcccactttt tgcagcagca gcagtgaacg accaagatta
    62461 gttggatcgt ttggcacggc aacctgcgaa ccatcctgca gttcatccag tgatttgatt
    62521 ttcttggagt aaccagcaat cggataaaca aaagtgttgc ctactgcgac cagtttgtag
    62581 ccacgatctt tcagttgctg atcaaggtac ggtttatgct ggaaggcgtt ggcgtcgata
    62641 tcgcctttgc tcaatgcttc gtttggcaga acatagtcgt tgaaggttac cagctcaacg
    62701 tccaggccat atttgtcttt cgcaactttc tgcgcgactt ctgctacctg ctgttcggca
    62761 ccaacaatca cgccgacttt aatgtggttt ggatcttttt catcctgacc gcagcctacc
    62821 agtgccagtg atccaatcag ggctcccact gccgcaaagg ttttgaattt gaacgccata
    62881 ccttattcct tcttcaatta tttatgttgt gttgaacgtt acttgcgagt gacagcccgg
    62941 acgatgcggt cgcctgcaaa ctgaattaaa taaaccagaa tgaccagcaa taccagtacc
    63001 gtattcatca ccgtcgcgtt gtagccgatg tagccatact gatagccaat ctgacctaaa
    63061 ccaccggcac cgactgcacc acccatcgcg gaataaccga ccagggtaat cagggtgata
    63121 gttgccgcat tcaccagacc cggcaacgct tccggtaaca gcactttaca gacgatctgc
    63181 attggcgtgg cacccattgc gcgggaagct tcaattaacc cggttgggat ctccagcaga
    63241 gcgttctcga ccatacgggc aataaacggt gctgcaccaa cggttaacgg aacaattgct
    63301 gcctgcaaac caatcgatgt accgacaata acgcgggtaa acggaatcat ccataccagc
    63361 aagataatga acgggatgga acggaaaatg ttcacaatcg cagaaacggt acgatacagc
    63421 ttcgcgttag caataatttg ccccggacgc gtgacataaa gcagaacgcc aaccggcaga
    63481 ccaatcacaa acccaaaaaa tccggatacg aaggtcattg ccagcgtttc ccatacgcca
    63541 cgaaccagca gccacatcat cggctcagac ataacccagt acctctactt ttacatggtg
    63601 ttcctgcagc caggcaatgg cggcttgcgt atcttgttgt gtgccgtgca tttcagtcag
    63661 catgatgccg aatttaacgc caccggcgta gtccatctgc gcgctaataa tgttgttgtt
    63721 gacgttgaaa cgacgcgcgg tttcagaaag cagtggggca tcgaccgatt gaccggtaaa
    63781 ctccagacgc agcatcggca cgcagtcagt aaatggctcc gtttgcagac gttcctggta
    63841 atcttccggg atatccagat gcagggtcga ctgaataaac ttctgcgcca gcggcgtttt
    63901 cggatgcgag aacacttcac ttaccgtgtc ctgctcgatc agttcgccat tgctgatgac
    63961 cgccacgcaa tcacaaatac gcttcacaac gtccatttcg tgggtgatca acagaatcgt
    64021 caaacccaga cggcggttaa tgtctttcag cagttcgaga atagaacgtg tcgttgccgg
    64081 gtccagcgcg ctggtggctt catcacacag caatactttg ggattgctgg ctaatgcacg
    64141 ggcaatcgcc acacgttgtt tctgcccacc ggaaagattc gacgggtagc tatcatgctt
    64201 atcgccaaga ccaaccaatg acagcaattc cgtcacgcga cgtttgatct cgtctttcgg
    64261 tgtgttgtcc agctccagcg gcagagccac gttgccaaaa acagtacgcg aagagagcag
    64321 gttaaaatgc tggaaaatca taccaatctg gcggcgagct ttggtcaact cggattctga
    64381 cagcgtggtc aattcctggc catcgaccag cacgctaccc tcggttgggc gctccagcaa
    64441 gtttacacaa cgtataagcg tactcttacc cgcgcctgag gcaccgataa cgccataaat
    64501 ttgtccagct ggcacatgca ggctgacgtt gttcaacgcc tggatggtgc gggtgccctg
    64561 gtggaacact ttggtgatat tcgaaagttt tatcattgat tatttattat cgtcattaag
    64621 ttagtcgtag catctcgaat gcctgaaacg ggcaacgccg tcaatgaaat ggatgttaag
    64681 gcatccagac gtctaaatca atcaggttta tgcgaagagc actttcttgc aggtcgaaac
    64741 atgcgatact agcgtcccat gccttattaa ggagctataa aaggtggcga agagcgtacc
    64801 cgcaattttt cttgaccgtg atggcaccat taatgtcgat cacggctatg tccatgagat
    64861 cgacaacttt gaatttatcg acggtgttat tgacgccatg cgcgagctaa aaaaaatggg
    64921 ctttgcgctg gtggtagtaa ccaaccagtc tggcattgct cgcggtaaat ttaccgaagc
    64981 acagtttgaa acgctgaccg agtggatgga ctggtcgctg gcggaccgag atgtcgatct
    65041 ggatggtatc tattattgcc cgcatcatcc gcaggggagt gttgaagagt ttcgccaggt
    65101 ctgcgattgc cgcaaaccac atccggggat gcttttgtca gcacgcgatt atttgcatat
    65161 tgatatggcc gcttcttata tggtgggcga taaattagaa gatatgcagg cagcggttgc
    65221 ggcgaacgtg ggaacaaaag tgctggtgcg tacgggtaaa cctattacac ctgaagcaga
    65281 aaacgcggca gattgggtgt taaatagcct ggcagacctg ccgcaagcga taaaaaagca
    65341 gcaaaaaccg gcgcaatgat taaaagatga acggttgaaa taaaaatgca tttttccgct
    65401 tgtcttcctg agccgactcc ctataatgcg cctccatcga cacggcggat gtgaatcact
    65461 tcacacaaac agccggttcg gttgaagaga aaaaatcctg aaattcaggg ttgactctga
    65521 aataggaaag tgtgatatac gccacctcgc gacagtgagc tgaaagctgc gtcgcaactg
    65581 ctctttaaca atttatcaga caatctgtgt gggcactcga agatacggat tcttaacgtc
    65641 gcaagacgat aaatgaatac caagtctcaa gagtgaacac gtaattcatt acgaagttta
    65701 attctttgag catcaaactt ttaaattgaa gagtttgatc atggctcaga ttgaacgctg
    65761 gcggcaggcc taacacatgc aagtcgaacg gtaacaggaa gcgcttgctg cttcgctgac
    65821 gagtggcgga cgggtgagta atgtctggga aactgcctga tggaggggga taactactgg
    65881 aaacggtagc taataccgca taacgtcgca agaccaaaga gggggacctt cgggcctctt
    65941 gccatcggat gtgcccagat gggattagct tgttggtgg
//
LOCUS       DAJTQQ010000010        64055 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000010 DAJTQQ010000000
VERSION     DAJTQQ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64055
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.010"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            121..564
                     /locus_tag="OO309_000717"
     CDS             121..564
                     /locus_tag="OO309_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5845974.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            800..1618
                     /gene="ybhA"
                     /locus_tag="OO309_000718"
     CDS             800..1618
                     /gene="ybhA"
                     /locus_tag="OO309_000718"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR5845975.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1619..2677)
                     /gene="modC"
                     /locus_tag="OO309_000719"
     CDS             complement(1619..2677)
                     /gene="modC"
                     /locus_tag="OO309_000719"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR5845976.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2680..3369)
                     /gene="modB"
                     /locus_tag="OO309_000720"
     CDS             complement(2680..3369)
                     /gene="modB"
                     /locus_tag="OO309_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR5845977.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3369..4142)
                     /gene="modA"
                     /locus_tag="OO309_000721"
     CDS             complement(3369..4142)
                     /gene="modA"
                     /locus_tag="OO309_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5845978.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4309..4458)
                     /gene="acrZ"
                     /locus_tag="OO309_000722"
     CDS             complement(4309..4458)
                     /gene="acrZ"
                     /locus_tag="OO309_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR5845979.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4587..5375
                     /gene="modE"
                     /locus_tag="OO309_000723"
     CDS             4587..5375
                     /gene="modE"
                     /locus_tag="OO309_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR5845980.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5443..6915
                     /gene="modF"
                     /locus_tag="OO309_000724"
     CDS             5443..6915
                     /gene="modF"
                     /locus_tag="OO309_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR5845981.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7176..8192
                     /gene="galE"
                     /locus_tag="OO309_000725"
     CDS             7176..8192
                     /gene="galE"
                     /locus_tag="OO309_000725"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR5845982.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8202..9248
                     /gene="galT"
                     /locus_tag="OO309_000726"
     CDS             8202..9248
                     /gene="galT"
                     /locus_tag="OO309_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR5845983.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9252..10400
                     /gene="galK"
                     /locus_tag="OO309_000727"
     CDS             9252..10400
                     /gene="galK"
                     /locus_tag="OO309_000727"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR5845984.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10394..11434
                     /gene="galM"
                     /locus_tag="OO309_000728"
     CDS             10394..11434
                     /gene="galM"
                     /locus_tag="OO309_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR5845985.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11637..12389
                     /gene="gpmA"
                     /locus_tag="OO309_000729"
     CDS             11637..12389
                     /gene="gpmA"
                     /locus_tag="OO309_000729"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR5845986.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12556..13608)
                     /gene="aroG"
                     /locus_tag="OO309_000730"
     CDS             complement(12556..13608)
                     /gene="aroG"
                     /locus_tag="OO309_000730"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR5845987.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13924..14304
                     /locus_tag="OO309_000731"
     CDS             13924..14304
                     /locus_tag="OO309_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR5845988.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14418..15359
                     /gene="zitB"
                     /locus_tag="OO309_000732"
     CDS             14418..15359
                     /gene="zitB"
                     /locus_tag="OO309_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR5845989.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15356..16075)
                     /gene="pnuC"
                     /locus_tag="OO309_000733"
     CDS             complement(15356..16075)
                     /gene="pnuC"
                     /locus_tag="OO309_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR5845990.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16113..17156)
                     /gene="nadA"
                     /locus_tag="OO309_000734"
     CDS             complement(16113..17156)
                     /gene="nadA"
                     /locus_tag="OO309_000734"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR5845991.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17405..17480)
                     /locus_tag="OO309_000735"
     tRNA            complement(17405..17480)
                     /locus_tag="OO309_000735"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17445..17447),aa:Lys,seq:ttt)
     gene            complement(17484..17559)
                     /locus_tag="OO309_000736"
     tRNA            complement(17484..17559)
                     /locus_tag="OO309_000736"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17524..17526),aa:Val,seq:tac)
     gene            complement(17611..17686)
                     /locus_tag="OO309_000737"
     tRNA            complement(17611..17686)
                     /locus_tag="OO309_000737"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17651..17653),aa:Lys,seq:ttt)
     gene            complement(17689..17764)
                     /locus_tag="OO309_000738"
     tRNA            complement(17689..17764)
                     /locus_tag="OO309_000738"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17729..17731),aa:Val,seq:tac)
     gene            complement(17900..17975)
                     /locus_tag="OO309_000739"
     tRNA            complement(17900..17975)
                     /locus_tag="OO309_000739"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17940..17942),aa:Lys,seq:ttt)
     gene            complement(18140..18931)
                     /gene="cpoB"
                     /locus_tag="OO309_000740"
     CDS             complement(18140..18931)
                     /gene="cpoB"
                     /locus_tag="OO309_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR5845992.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18941..19462)
                     /gene="pal"
                     /locus_tag="OO309_000741"
     CDS             complement(18941..19462)
                     /gene="pal"
                     /locus_tag="OO309_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR5845993.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19497..20789)
                     /gene="tolB"
                     /locus_tag="OO309_000742"
     CDS             complement(19497..20789)
                     /gene="tolB"
                     /locus_tag="OO309_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR5845994.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20922..22163)
                     /gene="tolA"
                     /locus_tag="OO309_000743"
     CDS             complement(20922..22163)
                     /gene="tolA"
                     /locus_tag="OO309_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR5845995.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22228..22656)
                     /gene="tolR"
                     /locus_tag="OO309_000744"
     CDS             complement(22228..22656)
                     /gene="tolR"
                     /locus_tag="OO309_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR5845996.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22660..23352)
                     /gene="tolQ"
                     /locus_tag="OO309_000745"
     CDS             complement(22660..23352)
                     /gene="tolQ"
                     /locus_tag="OO309_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR5845997.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23349..23753)
                     /gene="ybgC"
                     /locus_tag="OO309_000746"
     CDS             complement(23349..23753)
                     /gene="ybgC"
                     /locus_tag="OO309_000746"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5845998.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23903..24196)
                     /gene="ybgE"
                     /locus_tag="OO309_000747"
     CDS             complement(23903..24196)
                     /gene="ybgE"
                     /locus_tag="OO309_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR5845999.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24196..24309)
                     /gene="cydX"
                     /locus_tag="OO309_000748"
     CDS             complement(24196..24309)
                     /gene="cydX"
                     /locus_tag="OO309_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR5846000.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24324..25463)
                     /gene="cydB"
                     /locus_tag="OO309_000749"
     CDS             complement(24324..25463)
                     /gene="cydB"
                     /locus_tag="OO309_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5846001.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25479..27047)
                     /gene="cydA"
                     /locus_tag="OO309_000750"
     CDS             complement(25479..27047)
                     /gene="cydA"
                     /locus_tag="OO309_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR5846002.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27896..30529)
                     /gene="mngB"
                     /locus_tag="OO309_000751"
     CDS             complement(27896..30529)
                     /gene="mngB"
                     /locus_tag="OO309_000751"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCR5846003.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(30547..32463)
                     /gene="mngA"
                     /locus_tag="OO309_000752"
     CDS             complement(30547..32463)
                     /gene="mngA"
                     /locus_tag="OO309_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR5846004.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            32633..33355
                     /gene="mngR"
                     /locus_tag="OO309_000753"
     CDS             32633..33355
                     /gene="mngR"
                     /locus_tag="OO309_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR5846005.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(33458..34327)
                     /gene="sucD"
                     /locus_tag="OO309_000754"
     CDS             complement(33458..34327)
                     /gene="sucD"
                     /locus_tag="OO309_000754"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR5846006.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(34327..35493)
                     /gene="sucC"
                     /locus_tag="OO309_000755"
     CDS             complement(34327..35493)
                     /gene="sucC"
                     /locus_tag="OO309_000755"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR5846007.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(35587..36804)
                     /gene="odhB"
                     /locus_tag="OO309_000756"
     CDS             complement(35587..36804)
                     /gene="odhB"
                     /locus_tag="OO309_000756"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR5846008.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36819..39620)
                     /gene="sucA"
                     /locus_tag="OO309_000757"
     CDS             complement(36819..39620)
                     /gene="sucA"
                     /locus_tag="OO309_000757"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR5846009.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39921..40637)
                     /gene="sdhB"
                     /locus_tag="OO309_000758"
     CDS             complement(39921..40637)
                     /gene="sdhB"
                     /locus_tag="OO309_000758"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR5846010.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40653..42419)
                     /gene="sdhA"
                     /locus_tag="OO309_000759"
     CDS             complement(40653..42419)
                     /gene="sdhA"
                     /locus_tag="OO309_000759"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR5846011.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(42419..42766)
                     /gene="sdhD"
                     /locus_tag="OO309_000760"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(42419..42766)
                     /gene="sdhD"
                     /locus_tag="OO309_000760"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR5846012.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42760..43164)
                     /gene="sdhC"
                     /locus_tag="OO309_000761"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42760..43164)
                     /gene="sdhC"
                     /locus_tag="OO309_000761"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5846013.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            43545..43652
                     /locus_tag="OO309_000762"
     CDS             43545..43652
                     /locus_tag="OO309_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846014.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43858..45141
                     /gene="gltA"
                     /locus_tag="OO309_000763"
     CDS             43858..45141
                     /gene="gltA"
                     /locus_tag="OO309_000763"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR5846015.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            45532..46098
                     /locus_tag="OO309_000764"
     CDS             45532..46098
                     /locus_tag="OO309_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5846016.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            46149..48608
                     /locus_tag="OO309_000765"
     CDS             46149..48608
                     /locus_tag="OO309_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5846017.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            48623..49354
                     /locus_tag="OO309_000766"
     CDS             48623..49354
                     /locus_tag="OO309_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5846018.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            49351..50427
                     /locus_tag="OO309_000767"
                     /pseudo
     CDS             49351..50427
                     /locus_tag="OO309_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            50579..51625
                     /locus_tag="OO309_000768"
     CDS             50579..51625
                     /locus_tag="OO309_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR5846019.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(51622..52413)
                     /gene="nei"
                     /locus_tag="OO309_000769"
     CDS             complement(51622..52413)
                     /gene="nei"
                     /locus_tag="OO309_000769"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR5846020.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(52449..53183)
                     /gene="pxpA"
                     /locus_tag="OO309_000770"
     CDS             complement(52449..53183)
                     /gene="pxpA"
                     /locus_tag="OO309_000770"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR5846021.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(53173..54105)
                     /gene="pxpC"
                     /locus_tag="OO309_000771"
                     /pseudo
     CDS             complement(53173..54105)
                     /gene="pxpC"
                     /locus_tag="OO309_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(54099..54755)
                     /gene="pxpB"
                     /locus_tag="OO309_000772"
     CDS             complement(54099..54755)
                     /gene="pxpB"
                     /locus_tag="OO309_000772"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR5846022.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(54778..55521)
                     /gene="ybgI"
                     /locus_tag="OO309_000773"
     CDS             complement(54778..55521)
                     /gene="ybgI"
                     /locus_tag="OO309_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR5846023.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            55792..57273
                     /gene="dtpD"
                     /locus_tag="OO309_000774"
     CDS             55792..57273
                     /gene="dtpD"
                     /locus_tag="OO309_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR5846024.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(57423..58841)
                     /gene="phrB"
                     /locus_tag="OO309_000775"
     CDS             complement(57423..58841)
                     /gene="phrB"
                     /locus_tag="OO309_000775"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR5846025.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(58838..59347)
                     /locus_tag="OO309_000776"
     CDS             complement(58838..59347)
                     /locus_tag="OO309_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR5846026.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            59476..59763
                     /locus_tag="OO309_000777"
     CDS             59476..59763
                     /locus_tag="OO309_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCR5846027.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(59818..60198)
                     /locus_tag="OO309_000778"
     CDS             complement(59818..60198)
                     /locus_tag="OO309_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846028.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(60201..63587)
                     /locus_tag="OO309_000779"
     CDS             complement(60201..63587)
                     /locus_tag="OO309_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5846029.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDAPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            complement(63606..63809)
                     /locus_tag="OO309_000780"
                     /pseudo
     CDS             complement(63606..63809)
                     /locus_tag="OO309_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        15638 a        17252 c        15604 g        15561 t
ORIGIN      
        1 gatccagctt ctgcaaccag gccattcaga actgaagtga agcgcagtcg actgttaatt
       61 gacgagtatc tgttaatcag gaaaatagca gacgtccaga acgaatggtt cggcctttgc
      121 atggatcttg cactggtaac aggacagcgc aaaggagatc tggcggctat gagatgggag
      181 gatataagag atagcaggct atacgtggaa caacaaaaaa caggggcaaa aattcgcatt
      241 tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact ggataacttg
      301 aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag gacaattgca
      361 gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga cccaccgcca
      421 tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg cagtgatttc
      481 actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg tgacggacga
      541 gggagggaat ggaaagacat ttagaattaa tttcgaatga tttcgaaatg ccacttgtaa
      601 ttaattgatt ataaagaatt aattcctaaa gagcatttgc ttcatgaatg ctcctttgca
      661 tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg ctgaattaaa
      721 acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga aaattcttaa
      781 ccttaatctg gaatacgcca tgaccacacg cgtgattgct ctcgacttag acggcacctt
      841 attgaccccg aaaaagacct tgcttccatc atcgatagaa gccctggccc gcgctcgcga
      901 agcaggctat caattaatca tcgtcacagg tcgccatcac gtcgctattc atccttttta
      961 tcaggcgctg gcgctggata cacctgctat ttgctgtaat ggcacctatt tgtatgatta
     1021 tcatgcaaaa accgtgctgg aagcggaccc aatgcccgtt aataaagccc tgcaactcat
     1081 tgagatgctg aatgaacacc acattcacgg tctgatgtat gtcgatgatg caatggtcta
     1141 tgagcacccg accgggcatg tcattcgcac gtctaactgg gcgcaaaccc tgccgccgga
     1201 acagcgtccg actttcacac aagtcgcttc tctggctgaa acgacgcaac aagttaacgc
     1261 cgtatggaag ttcgccctga cgcacgatga cctgccgcaa ttgcagcatt ttggtaagca
     1321 tgtcgaacat gaactgggac tggagtgtga atggtcctgg cacgatcagg ttgatattgc
     1381 acacggcggc aacagcaaag gtaaacgttt gacgaaatgg gttgaggcgc aaggttggtc
     1441 gatggaaaac gtcgtggcat tcggcgataa ctttaatgat atcagtatgc tggaagccgc
     1501 tggtacaggc gtggcgatgg gcaacgccga tgacgcggta aaagcgcgcg ccaacattgt
     1561 gattggtgat aacaccaccg acagcattgc ccagttcatt tatagccacc tgatttaatc
     1621 aggcggttat cgacacactt ttaatttgcg cgtacagcca caggccaggt ttgatcgcca
     1681 gttcatccct ggcccacggg ctgatacgcg cccacagcgt tttaccgccg acttccagct
     1741 ccacttccac ctggccgttg tcgtcataac tattaacaac ttttgcccgc aatacgttac
     1801 gaatgctggt ttgctgcggc ggttgtaaaa ccagagaaac atcggaagcc tgaatgcgga
     1861 tacgtagcgc agcttgcagc ggttcgccca gcttattgac ccacaaatgc tgatcgccca
     1921 gcgccagcgc ggtcatcgcg taatgcggat gatgctcaag caccgtcact ttcagaatgc
     1981 tactttgttg ctctttcggc agccacggat tcatcacgct actgccccac acttcctcca
     2041 gcgcgccaaa ggctttcacc tgaccgtttt ccagtaccat cactctgtct gccagatgga
     2101 ggatctcatc cagcgaatgg ctgacataca acatcggaat gttgatttcc cgtgtcagcc
     2161 gttgcagata aggcaacagt tcgcgtttac gcggaatatc cagtgacgcc agcggttcat
     2221 ccagcaacag caattccggt gctgtcagca aagcccgacc aatcgccacg cgctgttttt
     2281 cgcctccgga caggctgcct ggtaaacggt caagcaacgg ttcaatgcct aaaagcgcca
     2341 ccagcttatc gaactgatcg accatacttt tcgacatgcc gtagcgcaga ttgccacgca
     2401 ctttgtaatg cgggaacaac cgcgcatcct gaaaaacata gccaacacga cgcttttccg
     2461 gcgtcaggca gatacctttt tcggcatcat ttagtacccg cccattgagg acaatccgcc
     2521 ctttttgcgg gcgcgtcagt ccactgatgg cgttaatcag cgaagttttt ccggcaccgg
     2581 agacgccaaa gatagcagtg atgccattgg cgggcagcgt ttcattaatg gtcaggcaat
     2641 ggttgcccaa cgtctgggaa aaattcagct ccagcatgat tagcgccccg cccgttcacg
     2701 gctgattctg gccagccatt ctgaaatcaa cagggagatc atcgccagcg caatagaaat
     2761 aatgcacagt ctcgccgcgc cactttcccc gccgggggtc tggatcaggg tatacatggc
     2821 agaaggaatg gttcgcgttt cgctaggaat gttcgacaca aaggtgatgg ttgcaccaaa
     2881 ctcaccgaga gaacgagcaa aagccagtac cgtaccaaca ataattcccg gtaaggtcag
     2941 cggtaacgtg atagtaaaga aaacgcgcca gcgcccggca cctagtgttc ttgcggcctg
     3001 ttccagtttg acatcaaccc cttccagcgc cagacgaatt gcccgcacca tcagcggaaa
     3061 cgacatgacg gcggcagcga gaaccgcgcc gcgccagcta aaggcgaagg taataccaaa
     3121 ccagtcatac agacgttcac cgataaatcc gcgccgtccc atcgaaacta ataataagta
     3181 accgacgacc acgggcggta acaccagcgg tagatgcagt acgctgtcga gcagagcttt
     3241 gcccggaaac gtgcaacgca ccagtaacca ggcaaaaaag atcccaaacg gcaggctaaa
     3301 cagcacagcc agggaagaaa ctttcaggct taataaaact gcctgccatt ctggatcggt
     3361 cagtatcatt acttggttgt aaatccgtaa cgtttaaaga tttccgctgc ctgcggtccc
     3421 ttcagataat cataaaatgc tttcactgtc gcattgttat gcccttccac aacagcaacc
     3481 ggatattcca cttttttatg tgaatcttcc gggaaggtgg caaccacttt tacccctttg
     3541 ctggcaactg cgtcagaacc gtagacaatg cccagaggcg cttcgttacg ttcgaccagc
     3601 gccagcgccc cacgaacatc ttccgctggg gccagtttcg gagagagcgt atcccatgcg
     3661 cccagttttt gcagtgcttc ttttgcataa atgccagcgg gaacatgttc cggatcgcca
     3721 accgccaggc gaccgccatt cagcagcgaa gtccagttag ttttgctgtc gatggtgaaa
     3781 tctttctgct cgctggcttt cggtgctaca acgaccaggc tattgccgag cagtgtctga
     3841 cgcgtagcag tatcgatcgc ttttttatca accgcataat ccatccattt ctgatcggca
     3901 gaaataaaca gatccgcagg ggcacccgct tcaatctgac gggcgagagt agatgacgaa
     3961 gcgaaagaag aaaccacatc cacgcctttc tcttttttat actgcgtagc aatgtcctgc
     4021 attgcgttag tcagtgatgc ggcggcgaac accgtgattt tcccttcatc tgccagtgca
     4081 ttgccagcaa ctgcgaaaga gagtgccgcc ccggcaaaca agttcaacca tttacgagcc
     4141 attggtaacc ccttaatgta acgttatgta ggcgacaata taacgactaa ctcggaattt
     4201 tcccagcagt tattgctaac ctttgtgagg tagataagaa aaaatatcgg caggaaaagc
     4261 aggaagttga gaaaaagaaa atgcccgact aagcgggcac tcagggaatc aatgattttg
     4321 tccgggctgg tcttttttac caacaccaga aaagatgttg aatacttcac caagaccgta
     4381 aatcagaccc aggatgatgg ccatcacgac aggtaccatg attacggcga ataccagact
     4441 ttttaataac tctaacatgg tcaactccag atatagtcat gagactattc taaccgctaa
     4501 gcacagaaaa gcactcccct tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac
     4561 aataagactt ttgccaggac attgttatgc aggccgaaat ccttctcacc cttaagctcc
     4621 aacaaaaatt attcgccgac ccgcgccgca tttcgctact aaaacacatt gcgctttccg
     4681 gttccattag ccagggagcg aaagatgccg gtatcagcta taaaagcgcc tgggatgcca
     4741 ttaacgagat gaatcagtta agtgagcata ttctggtcga gcgcgcaaca ggcggtaaag
     4801 gtggcggagg cgcagtactg acccgctatg gtcagcgact gattcagctc tatgacttac
     4861 tggcgcaaat ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc ctgccgctga
     4921 acagcctgct ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt aaccagtggt
     4981 tcggtaccat caccgcccgc gatcatgatg acgttcaaca gcatgttgat gtcttactgg
     5041 ctgacggaaa aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc
     5101 tggatgaagg caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc attactcagg
     5161 acgaagcggt cgcacaaaac gccgacaacc aattaccggg tattattagt catattgagc
     5221 gcggcgcaga gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca
     5281 cagtgccggt aaatgaagcg acttctcttc agcaagggca gaatgtcacg gcctacttta
     5341 atgccgacag cgtgattatc gccacgctgt gctaagcgtg ttgacaattt gttatgaaac
     5401 acgtatccct gtcagtaatc gctgcacaaa atgggatata aaatgtcatc gttgcaaatt
     5461 ttgcaaggca cgtttcgtct tagcgacaca aaaacgctgc aattgcctca gctaacgtta
     5521 aacgcgggtg atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc ggcgctggcc
     5581 cgcgcgctgg cgggggaact tccgcttttg aaaggtgaac ggcaaagcca gttttcccac
     5641 atcactcgtc tctccttcga gcaattgcaa aagctcgtca gcgacgaatg gcaacggaat
     5701 aacaccgata tgctcggccc tagcgaagat tacaccggac gcactacggc tgagattatt
     5761 caggatgaag taaaggatgc accgcgttgc atgcaactgg cgcagcagtt cggtattacc
     5821 gccctcctcg accgacgctt taaatacctt tccactggcg agacgcgaaa aaccctgctg
     5881 tgtcaggcgc tgatgtcgga gcctgacttg ttgattcttg atgagccgtt cgatggcctg
     5941 gatgttgcct cacgtcagca gctggctgag cgactcgcct cgttacatca gtccggtatt
     6001 actttggtac tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca gtttgctggc
     6061 gtgctggcgg attgtacgtt agcggaaact ggcgctaaag aggaactgct ccagcaagca
     6121 ctcgtcgcgc aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc ggagccggat
     6181 gaaccttcag cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct gaacaatggc
     6241 gtggtttctt ataatgatcg ccccattctt aataacctta gctggcaggt gaatccaggc
     6301 gaacactggc aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt aagcctgatt
     6361 actggcgatc atccgcaagg ttacagcaac gatttgacgc ttttcggccg acgtcgcggc
     6421 agcagcgaaa ccatctggga catcaaaaag catatcggtt acgtcagcag tagtttgcat
     6481 ctggattacc gggtcagcac taccgtgcgt aatgtgattc tttctggcta ttttgattcg
     6541 attggcattt atcaggtcgt ttcggatcgc cagcaaaaac tggtgcagca gtggctggat
     6601 attctcggca ttgataaaca cacggctgac gctccgttcc atagtctttc ctggggacag
     6661 cagcgtttgg cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct tattctcgat
     6721 gaaccactac aggggcttga tccgctcaat cgccaactta tccgccgttt tgttgatgtg
     6781 ctgattagcg aaggtgaaac gcaattgttg tttgtttcgc accacgctga agatgcgcct
     6841 gcctgtatta cccatcgcct tgagttcgtg ccggacggtg gactctatcg ctatgtgctg
     6901 acaaaaatat attgagtcgg tagtgctgac cttgccggag gcggcctaag cgcactctcc
     6961 ggccaacggt tcgacgcatg caggcatgaa accgcttctt ttttcagata aaaagcgcaa
     7021 tcattcataa accctctgtt ttataatcac ttaatcgcgc ataaaaaacg gctaaattct
     7081 tgtgtaaacg attccactaa tttattccat gtcacacttt tcgcatcttt gttatgctat
     7141 ggttatttca taccataagc ctaatggagc gaattatgag agttctggtt accggtggta
     7201 gcggttacat tggaagtcat acctgcgtgc aattactgca aaacggtcat gatgtcatca
     7261 ttcttgataa cctctgtaac agtaagcgca gcgtactgcc tgttatcgag cgtttaggcg
     7321 gcaaacatcc aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg accgagatcc
     7381 tgcacgatca cgctatcgac accgtgatcc acttcgccgg gctgaaagcc gtcgccgaat
     7441 cggtacaaaa accgctggaa tattacgaca acaatgtcaa cggcactctg cgcctgatta
     7501 gcgccatgcg cgccgctaac gtcaaaaact ttatttttag ctcctccgcc accgtttatg
     7561 gcgatcagcc ccaaattcca tacgttgaaa gcttcccgac cggcacaccg caaagccctt
     7621 atggcaaaag caaattgatg gtggaacaga tcctcaccga cctgcaaaaa gcccagccgg
     7681 actggagcat tgccctgctg cgctacttca acccggttgg cgcgcatccg tcgggcgata
     7741 tgggcgaaga tccgcaaggc attccgaata acctgatgcc atacatcgcc caagttgctg
     7801 taggccgtcg cgactcgctg gcgatttttg gtaacgatta tccgaccgaa gatggtactg
     7861 gcgtacgcga ttacatccac gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa
     7921 aactggcgaa caagccaggc gtacacatct acaacctcgg tgctggcgta ggcagcagcg
     7981 tgctggacgt ggttaatgcc ttcagcaaag cctgcggcaa accggttaac tatcattttg
     8041 caccgcgtcg cgaaggcgac cttccggcct actgggcgga cgccagcaaa gccgaccgtg
     8101 aactgaactg gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc tggcactggc
     8161 agtcacgcca tccacaggga tatcccgatt aaggaacgac catgacgcaa tttaatcccg
     8221 ttgatcatcc acatcgccgc tacaacccgc tcaccgggca atggattctg gtttcaccgc
     8281 accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc agccaaacag gtgttacctg
     8341 cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc
     8401 ccgattacac cgggacttac gttttcacta atgactttgc ggccttgatg tctgacacgc
     8461 cagatgcgcc agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc
     8521 gggtgatctg cttttcaccg gatcacagta aaacgttgcc agaactgagc gttgcggcat
     8581 tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt
     8641 gggtgcaggt ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg catccgcacg
     8701 gacagatttg ggcaaatagc ttcctgccta acgaagctga gtgcgaagac cgcctgcaaa
     8761 aagaatattt tgccgaacag aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg
     8821 cagacggtag ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg ccttactggg
     8881 ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg atcaccgatt
     8941 tgaccgacgc ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc agtcgttatg
     9001 acaacctctt ccagtgctcc ttcccctact ctatgggctg gcacggcgca ccatttaatg
     9061 gcgaagagaa tcaacactgg cagctgcacg cgcactttta tccgcctctg ctgcgctccg
     9121 ccaccgtacg taaatttatg gttggttatg aaatgctggc agagacccag cgagacctga
     9181 ccgcagaaca ggcagcagag cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg
     9241 gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac gcatttggct
     9301 accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt gaacacaccg
     9361 actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg
     9421 caccacgcga tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat cagctcgacg
     9481 agttttccct caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg aactacgttc
     9541 gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggtgtg gacatggtga
     9601 tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg
     9661 tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa atcgcgctta
     9721 acggtcagga agcagaaaac cagtttgttg gctgtaactg cgggatcatg gatcagctaa
     9781 tttccgcact cggcaagaaa gatcatgcct tgctgattga ctgccgctca ctggggacta
     9841 aagcagtttc catgccgaaa ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta
     9901 ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct
     9961 tccagcagcc agctctgcgc gatgtcacca ttgaagagtt caacgctgtt gcacatgagc
    10021 tggacccaat cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc cgcaccgttg
    10081 aagctgccag cgcgctggag caaggcgacc tgaaacgtat gggcgagttg atggccgagt
    10141 ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac actctggtag
    10201 aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc ggcggatttg
    10261 gcggctgtat cgtcgcgctg atcccggaag agctggtgcc tgccgtacag caagctgtcg
    10321 ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt aaaccatcac
    10381 aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggtc agccgtaccg
    10441 actgttaact ttgcgtaaca acgcagggat ggtagtcacg ctgatggact ggggtgcgac
    10501 tttactttcc gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg
    10561 tgccagtccg gaatgctatc aggatcaggc tgcgtttctg ggggcctcta ttggtcgtta
    10621 tgccaaccgc atcgccaata gtcgctatac ctttgacggt gaaaccgtga cgctttcgcc
    10681 aagccagggc gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca
    10741 gattgtgaac cagaacgatc gtcaggtgct gtttgccctg agttcagatg atggcgatca
    10801 gggcttcccg ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg ataaccgtat
    10861 ctccattact tatcgcgcca cagttgataa accttgcccg gtgaatatga ctaatcacgt
    10921 ctatttcaat cttgacggcg agcagtctga cgtgcgcaat cacaagttgc agattctggc
    10981 ggacgaatat cttccggttg atgaaggcgg cattccgcac gacggcttga aatctgtcgc
    11041 cggaacgtct tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga
    11101 cgatcagcgc aaagtgaaag gttacgatca cgcattcttg ttacaggcca aaggcgatgg
    11161 caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga aggtctacac
    11221 caccgctccg gctcttcaat tctactccgg caacttcctc ggcggcacac cgtcgcgggg
    11281 aaccgaacct tacgccgact ggcaagggct ggcgttggaa agcgagtttc tgccggacag
    11341 cccgaaccac cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc
    11401 cagcctgacg gaatatcagt ttattgctga gtaatgttaa gccctcgtca tgagggcttt
    11461 atcttcatat tgttcaaatc accagcaaac accgacatat ttgcaactca atattcacaa
    11521 caaccttaca ctgcgccact attttcgcta tggttatgcg taagcattgc tgttgcttcg
    11581 tcacggcaat ataatgagaa ttattatcat taaaagatga tttgaggagt aagcatatgg
    11641 ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa gaaaaccgtt
    11701 tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca aaagcagcag
    11761 gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacttct gtgctgaaac
    11821 gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg ctgcccgttg
    11881 agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg aacaaagcgg
    11941 aaactgctga aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt tttgcagtga
    12001 ctccgccgga actgactaaa gatgatgagc gttatccggg tcacgatccg cgttacgcga
    12061 aactgagcga gaaagaattg ccgctgacgg aaagcctggc gctgaccatt gaccgcgtga
    12121 tcccttactg gaatgaaacc attctgccgc gtatgaagag cggtgagcgc gtaatcatcg
    12181 ccgcacacgg taactccctg cgtgcgctgg tgaaatatct tgataacatg agcgaagaag
    12241 agattcttga gctgaatatc ccgactggcg tgccgctggt gtatgagttc gacgagaatt
    12301 tcaaaccgct gaaacgctat tatctgggta atgctgacga gatcgcggcg aaagcagcag
    12361 ccgttgcgaa ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg agaaagccga
    12421 ctgcaagtga gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat tgcctgatgc
    12481 gacgctgcgc gtcttatcag gcctgtggtg attcatcgga tacgccactc tgacggcgca
    12541 tccgacaatt aaaccttacc cgcgacgcgc ttttactgca ttcaccagtt gacgtaacag
    12601 agcatcggta tcttcccagc caatgcaggc atcggtgatg ctcttaccgt aggccagcgg
    12661 ctccccgcta tcgaggctct gattgccttc caccagatgg ctttccacca tcacgccaat
    12721 aatggccttt tcgccaccgg caatctgctg gcaaacgtca gcacaaacat ccatctgctt
    12781 tttgaattgt ttggacgaat tagcatggct gaaatcgatc atcacctgag ctggcagacc
    12841 tgctttgttc agcccttctt tcacttcagc aacgtgcttc gcgctgtagt taggctcttt
    12901 accgccgcgc agaatgatat ggcaatcgcc gttaccactg gtattcacaa tcgccgaatg
    12961 cccccattta gttacggaca ggaagcagtg cggcgcaccg gcggcattaa tggcatcgat
    13021 agccacttta atcgtaccgt ctgtgccatt tttaaaacct accggacaag aaagaccaga
    13081 cgccagttcg cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc cccagctcat
    13141 caggtcagcg agatactgag gagtgatcat atcgagaaac tcacccgccg ctggcagacc
    13201 gctgtcgtta atatcaagca gcaatttacg ggctatacgc agaccgtcgt tgatctggaa
    13261 gctgttatcc atatgcggat cgttaatcaa ccctttccag cccaccgtgg tacgcggctt
    13321 ttcaaaatag acgcgcatta cgatttccag ctcatctttc agctcttcac gcagcgccag
    13381 caagcgagtg gcatactctt tagccgcgac aggatcatga attgagcacg ggccaatcac
    13441 caccaacagg cgatcatcat tacctttcag gatcttatgg atcgcttttc gggcatgggc
    13501 aaccgtattc gcggcatttt cagttgcggg gaatttttcc agcaatgcga caggaggaag
    13561 taactctttg atttctttga tgcgtaaatc gtcgttctga taattcatgt ctgttccagt
    13621 gttgccatac ttatcttagt gaatgcaata cttccaatct atatcttccg tcagaatgtg
    13681 taaacggggt tttacactat gaacggatta atcctggaat tagcaagaaa aacgccagat
    13741 tgtcgcgaaa aacgagatct ctcctacaat ttctaactgt aactcctttg tttgttaatt
    13801 atttcaagat tctctgctgc gtttcataaa ctggctgaaa tcttaaacca atgccttata
    13861 ttcacctgca aatgcactgt tggaagaggt tatccgacat aacgaccata acaggagcat
    13921 cctatgaaaa tgacaaaact ggccacatta tttctgactg ccactctaag ccttgccagc
    13981 ggtgccgcac tggctgccga tagcggagcg caaactaata acggccaggc aaacgccgca
    14041 gctgatgcgg gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa taacgtcgac
    14101 aataacgggg taaataccgg ttctggcggc acaatgctgc attcggatgg ttcttcaatg
    14161 aacaatgacg gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa agatggtcga
    14221 tgtccggata tcaataaaaa ggtacaaacc ggtgatggca tcaataatga tgtcgatacc
    14281 aaaaccgacg gtaccacgca gtaaccctgc aaaatagcca ataatgggag agcggagtct
    14341 ctccctttta atttttcact tctaaaagca gtatgatgtc ataaagttaa gtgagaataa
    14401 ttaagggatg actcgtcatg gcgcactcac actcacacac gtcctcacac ctgccagaag
    14461 ataataatgc tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt atgctggtag
    14521 aagtcgttgg tggttttctt tctggttctc tggcattgct ggccgatgcg ggtcatatgt
    14581 tgaccgatac tgccgccctg cttttcgccc tactcgccgt gcaattttca cgtcgtcctc
    14641 ccaccattcg ccacactttc ggttggctaa gactgaccac gctcgcggcg tttgtgaatg
    14701 ccatcgccct cgtggtaatt accattttga ttgtctggga ggcgatagaa cgcttccgca
    14761 cgccgcgtcc ggtcgaggga ggcatgatga tggcaattgc cgtggctggg ttgctggcaa
    14821 atatactttc tttctggtta cttcatcacg gcagcgaaga gaaaaacctc aacgtacgag
    14881 cagcggcact gcatgtgctc ggggacctgc tgggttcggt tggggctatt attgccgcgt
    14941 tgattattat ctggactggc tggacacctg ctgaccccat tctctcgata ctggtgtcgc
    15001 ttctggttct gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa ttacttgaag
    15061 gtgcaccggt atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg gaaatcccgg
    15121 aagtccgcaa tgtgcaccat gtacatgtat ggatggtagg cgagaagccg gtgatgacgt
    15181 tgcatgtgca ggtgatcccg ccacacgatc acgatgcctt gttggatcag atccaacact
    15241 acctgatgga tcactatcag attgagcaca ccaccattca gatggaatat caaccttgtc
    15301 ataggccaga ctgccacctg aatgaagggg tgtctggcca ttcacatcat caccattaat
    15361 gggacagcgc gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg ctgccgttga
    15421 gcgcaataaa ggtcaggatg atgtactcca gagacatggc gtaaacgccc tgaagtgcaa
    15481 aaataacgac gctaatcacg ttaataatca cccacaacag ccagttttcc acatacttac
    15541 gcgtcatcag aatcattgcc acgatagata acaccatcat gcatgaatcc cagaacggga
    15601 aagcgtccgg ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc atgatcatga
    15661 ctgccacgcg ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc agaccaatcg
    15721 aaacaacgca aaccgccaac cagctgagtg ccttcggcaa tggcaaccag cgaattttca
    15781 actccgcctc gttctgactg gtttgtcgcg accacgcata ccagccgtaa atattcgcgg
    15841 caaagaaaaa cacctgtaat agcaggctgg catacagctg aatctgaaag aaaataatgc
    15901 caaacaaggt aacgttgatc aggccaaaga agtagttgct gatcttctcc agactggcaa
    15961 ggccaataca cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag agatcataac
    16021 cgcccgcccc tattggtata tgtaccagga tattctgcac actaaaaaaa tccatctttc
    16081 ccccaaagcg taacgttacg ccttattatt cgttatccac gtagtgtagc cgcaaaatcc
    16141 agcatacgat tgagcggcac cagcgccctc tctcgcagcc tctcatcgac atgaacctcg
    16201 tgattacttc cttcctgttc taatgcctct gcgatagcct gaaggtcatt catggccatc
    16261 cacggacaat gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg tgcttccagt
    16321 aactctttat ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc ggttgccaca
    16381 ataagcctct gatgaggcaa tgcttttgca gcagcgatca gttgactggt ggaaccgacc
    16441 gcatccgcca tatcgacaat agcttgtggt gattctggat gcaccagtat ggcagcatcc
    16501 gggtattctt cttgcaagcg ggttaacgcc tgagtcttaa attcatcatg cacaatgcag
    16561 gcaccctgcc agcataaaat gtctccaccc gtctgttttt gcacgtaacg ccccagatgt
    16621 ttgtcgggtg cccagatgat tttttcaccc aaactatcaa gatgatcaat aagttcgacg
    16681 gcaatgcttg aagttaccac ccaatctgcg cgcgctttta ccgcagcaga agtgttggcg
    16741 tagacgacga cagtacgatc gggatgggca tcgtaaaatg cgttaaattc ttcaacaggg
    16801 cagccgagat ccagtgaaca ctcaacctga agtgtcggca tcagaattgt tttttccgga
    16861 ctgagaattt tggcggtttc tcccataaat ctcaccccag cgactaacaa agtagaagcg
    16921 ggatgctttg caccgaagcg cgccatttcc agagaatcag aaatacagcc accggtttct
    16981 tctgccagtt gttgaatttc gggatcggta tagtagtggg caaccatcac cgcattacgt
    17041 tcttttagca gacgttttat cttctcgcgg taatacgctt tttcatcaat gcttaacggc
    17101 gtcggcttcg gggggaaagg ataaatcgcc gtgtctggat caaacattac gctcatctta
    17161 ccatctcgtt ttacaggctt aacgttaaaa ccgacattag cgtaacattc gctgttatcg
    17221 gtgtaatttg ttttatatgc taaacaagat agtcgaatgg tggggaaaag tcactcgaat
    17281 tacgcctaat tccaccgacg tagagttgct tgcacagtaa gctctgacga tgtcatcagg
    17341 tttacaaagt gaaattgagg atgcctgaat agcaaaaaag cgcctttagg gcgctttttt
    17401 gcattggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt caactgctct
    17461 accaactgag ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc cgaccccctc
    17521 cttgtaaggg aggtgctctc ccagctgagc taatcacccg atactgcgct ggatactaca
    17581 aactacttcc accgaaccca ccgtaattag tggtgggtcg tgcaggattc gaacctgcga
    17641 ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccggtg gtgggtgatg
    17701 acgggatcga accgccgacc ccctccttgt aagggaggtg ctctcccagc tgagctaatc
    17761 acccgacact acgctggata ctacaactaa ctatgttagt gggggtcgtg caggatgact
    17821 cgacttcgtc tcgcccttcg gcgccgttgc tgatgcaacg ttatccttca cgttttacta
    17881 ccgcttccac cttagcgatt ggtgggtcgt gcaggattcg aacctgcgac caattgatta
    17941 aaagtcaact gctctaccaa ctgagctaac gacccacttt ttcgttgctt tcggtttgtt
    18001 tgatatcccg tggcaacggc ggcatatatt actgatttca gacttgagcg caacaaaaat
    18061 ttcgatgcag atcactcaac tgcttatgat tcgcacgaca cgaccagaaa taatgcgact
    18121 tctggtcgtg tgttatgcat tacatcgcgt tcagacgttt ttgtgcctgt ttagcgccat
    18181 cagtaccagg gtatttactg ataacctgct ggtatacggc tttcgctttt gcggtgtcac
    18241 ctttgtcctg catgatgacg ccgactttaa acatcgcatc agcagccttt ggtgacttcg
    18301 gatagttttt cactaccgaa gcaaaatagt acgccgcatc atctttttta cccttgttgt
    18361 agtttaactg acccagccaa taattggcgt ttggcaggta agttgaatca gggtaatttt
    18421 tgatgaaatt ctgaaatgcc accattgcgt catcctggcg ggatttatcc tgcaccagcg
    18481 caatagctgc attgtaatcc gtgtttgcat cgccgctttt caccggcgcg ccagcattcg
    18541 cagtaccagc atcagctgtc ggcgtcgttg atgccgtcgc accgctttga tcgctgctgg
    18601 ttgattgcgc cgctgcacca ccgctgctga ggctgtcgat ctgcaacagg atctgctttt
    18661 gccgctccac gacctgattc agttgatact gattttcctg aatctgacca cgcagggaat
    18721 caatatcgga ttgattatca gaaagttgtt gctggagttg ggttaaaagc tggctgtgag
    18781 cattagaaat acgctcaagt tgagtgacgc ggtcttcgac cgagcctgag ctgacactac
    18841 tgattggtgc ctgagcaaaa gcggcccagg gggccgctat accaaccagt aacgacagac
    18901 tcaatagttg atgtctgaag ttactgctca tgcaattctc ttagtaaacc agtaccgcac
    18961 gacggttttt ggagtatgcc gcttcgtcat gacccagtac tgcaggtttt tctttaccgt
    19021 aagaaacgat ggagatctgg tctgcagaaa cgcctttacc ctgcaggtac atcttaacgg
    19081 cgttcgcacg acgttcaccc agggagatgt tgtattccgg agtaccacgt tcgtccgcgt
    19141 gaccttctac ggtgactttg taagacgggt tgctacgcag gaagtttgca tgtgcatcca
    19201 gcatttgagc gaagtcagaa cggatatcgt acttgtccag atcgaagtaa acgatgttgt
    19261 tctgctgcag ctgttgcatt tgcagacgag cctgctcttc ggaagacatg ttgccgttgc
    19321 cgccgttcgc atccatacca gtgccggcac ccagcatgcc ttcgctgccg tcattgctgg
    19381 cgttcttgtt ggaagaacat gccgcaattg ccataacagg cagagcaatc atcagccctt
    19441 tcagcacttt gttcagttgc atttcaatga ttcctttact attcaattaa ttattatcac
    19501 agatacggcg accaggcagg gaatttgacc tgtccatcag ttgccggaag acgcgctttg
    19561 aaacgcccat ctgtagaaac caaattcagc acggatccca tcccctgaga agagctgtag
    19621 attaccatag tgccgttagg tgccagactt ggcgtttcat ccaggaacgt ggacgacaga
    19681 acttgtacgc ctcccgttgc cagatcttgt ttggcaatgt gctgctgccc accattggag
    19741 ctgaccatta ccataaattt accgtcgctg ctgacatccg catcctggtt ctgcgaacct
    19801 tcccaggtaa tacgttgtgg cgcaccgccg ttgatattca ctttataaac ctgcggacga
    19861 ccggcctggt cagaagtaaa tgccaggttc tggctatccg ggaaccaggt cggttcggta
    19921 ttgttactgc gaccatcagt cacctggcgg atctgaccag aagccaaatc cattacgtac
    19981 aggttcagac taccggtttt cgacaaggcg aatgccagtt tgctgccgtc tggcgagaat
    20041 gcaggtgcac cgttgtgacg cgggaatgaa gccacctgac gtacagcgcc atttgccagc
    20101 gtctgaataa ccagcgcgga acgaccgctt tcgaaggtca cataagccag tttagaaccg
    20161 tctggtgacc acgccggaga catcagcggc tgcggtgaac ggtgaacgac aaactggttg
    20221 taaccgtcat agtcagatac gcgcagttca tacgggaact gaccgccgtt ggtctgaaca
    20281 acgtaggcaa tacgggtacg gaacgcacct ttaatgccgg tcagtttttc aaacacttca
    20341 tcactggcgg tatgaccagc ataacgcagc cactgcttgt tcactttgta cgagttctga
    20401 gcaagtacag tacccggtgc gccgccagtg tcaacaagtt gataagcaac attgtaagaa
    20461 ccatccggat tcggagtgac ttgaccgaca actacagcgt caatgcccag tgcggaccat
    20521 gcagctggtt gtacttcctg cgcactaccc ggctgctgtg gcagacgagc gcgatctaac
    20581 ggattaaatt taccgctgtt acgcaagtca gcagcaacga tgccgccaat atcttcaggt
    20641 gccgcaccag gccccgccca ctggaaagga acaacaccaa taggacgacc ggaatctaca
    20701 ccgctgtcga tcacaatgcg gacttcagca tgcagaactg atgcccacag tatgagaaaa
    20761 ccaaatgcta ctcgtaatgc ctgcttcatc atatctccct tatctggacc gatggcccac
    20821 gataatttag cagaatgtta acaaactaaa atacacaaaa ctaccagaac cccgtggcaa
    20881 ccggtgcctg atgttgaccg tccgaacagt caacatcgcg attacggttt gaagtccaat
    20941 ggcgcgtttt tgaacacttc atatactgcc tggcttggtg gtttcgggat cttcgcaagt
    21001 ttagctgctg ccaacgcagc ctgacaaagt gcgggatcgc caccttcagg tttgatatcc
    21061 agtaacatac catcgggtgc cagttttatg cgcagcgtac aggttttgcc tgcataggac
    21121 gatgcgtcat agaacttact ttcgatagca gatttaatct gcccggcata gttattgata
    21181 tcggcccctg atgcgccatt gtttttagta ttaccactcc cggcaggcga agcattgttc
    21241 cctttcgccc ctccccccgt tttcggtgca ttcttaccag agcttagctc accgaaaata
    21301 tcatctgcct ctgcggcagc ttttgctgca gcggcttttt cagctgctgc ttttgccgct
    21361 gccgcttttt tgtctgcggc ggctttttct gccgctgctt tcttatcagc tgctgcctta
    21421 tcagcagccg ctttttctgc tgcttttttg tcggctgcag ctttctctgc tgccgctttc
    21481 ttatcagctg cagccttttc agcagccgct ttcttctcag cttctgcctt ggctttttca
    21541 gcagcttcag ttgccgcttt ctttcttgct tcagctgcag ctgcttctgc cgcttccgct
    21601 ttcttcttca gtgccgcagc ggctacctcg gcttttttct gcgcttcggc tgcggctttg
    21661 gcggcttctg cttctgcttt tttcttcgcg tctgcagccg ctttcttcgc tgcttcttcc
    21721 gcagctttag catctgccgc cgctttcgct gccgccactt cagcttgctt ctgctttaac
    21781 tcggcctgtt ttgcggcttc ttcagcctgc tttttctgct cctgagccgc taaccgctct
    21841 ttctcaagtt gcttcaggcg ttcctgttca gccgcttgtt tctcacgcag ttcttcagca
    21901 gcctgctgtt ccttcatctt gcgctgttca tcagaacgct tcgcgcttga ttcctggctt
    21961 tgcatgcgtt tgtactgctc aactaccgca cctgaatcaa ccatgacagc gtcgatggac
    22021 gaaccaccgc cgcctccggc tgaagcttct atattctcat cgaacgaact ccagatcagc
    22081 gccgcaaata agatgacatg cagcactgct gaaataatta tcgcccgctt gagcttgtcg
    22141 ttttgttcgg ttgcctttga cactctcggt ttccaaaaac tgttcgcctg ttacccgctc
    22201 tctttcaagc aagggaaacg cagatgttta gataggctgc gtcattaaac caaccgattt
    22261 cacacccgca ctatgtaaca agttcagtgc tttaattatt tcatcgtaag gcacatcttt
    22321 tgcgccaccg atcagaaaga ccgttttcgg gttggccttg aaacggctgg acacttccgc
    22381 caccacctgc tctggtggta aacgctccag gcgatctttc tcaaccacca cggtgtactg
    22441 accaatacca gacacttcaa caatcactgg cggattatcg ttactgctca ccgcctgtga
    22501 ttcagtagcg tctggcagat cgacctccac gctctgggtg atgatgggcg ctgtcgccat
    22561 aaagatcagc aacagcacca gcagtacgtc cagcaacgga acaatgttga tttcggactt
    22621 gagatcgcga cgacctcgtc cacgcgctct ggccatggct taccccttgt tgctctcgct
    22681 aacggtaaac gcctggcggt gcagaatcgc ggtaaactct tccataaagt tgtcgtaatt
    22741 cagttccagt ttgtttacgc gctggttgag acggttataa gccattaccg ccggaattgc
    22801 ggcgaacaga ccaattgccg tcgcaatcaa cgcttctgcg ataccgggcg caaccatttg
    22861 cagtgttgct tgttttaccg ccccgagggc gataaaggcg tgcatgatcc cccagaccgt
    22921 accaaacaga ccaatatacg ggctgatgga gccaaccgta ccgaggaacg gaatgtgcgt
    22981 ttccagattt tcaagttcac ggttcatgga gatacgcata gcacgcgacg ccccttccac
    23041 tacggcttcc ggcgcatggc tattggcacg atgcaggcgc acaaactctt tgaacccgct
    23101 gtaaaagatt tgttccgaac cagtcagatt atcccgtttc ccctgactct cttgatagag
    23161 gcgagagagt tcgattccag accagaattt atcttcaaac gcttcggctt cacgcgccgc
    23221 tgcgttaaga atacgggttc gctggataat aatggcccag gatgcgattg aaaaaccaat
    23281 caaaatcaac atgataagtt taaccagaag gctagccttc aggaacaaat caaggatatt
    23341 catgtcagtc actgcttaaa ctccgcgaca atagacttgg gaagcgcacg aggcttcatt
    23401 ttgagtgggt caacgcaaac aaccagaacc tctgcttcat tcagcaaagt attctcggcg
    23461 ttgacaatac gttgcgtgaa aaccaaagag gtgccacgca ttgatgttat ttcagtctgt
    23521 atttcgagca tatcgtcgag ccgcgcaggt gcgtaatatt ccaccgtcat tttacgtacc
    23581 acaaaggcaa cgcgttcagc catcagcgcc tgctgactga agtgatgatg acgcagcatc
    23641 tctgtgcgtg ctctttcata aaaagcgacg taactggcgt ggtacaccac accaccggca
    23701 tcggtatctt catagtagac gcgaaccggc catcgaaaca gcgttgtatt cactttacat
    23761 cccggtaatg caacaaaagt tagagctttt aaacgctgct actatacgcg cgcgattgag
    23821 gtttgggaat gagggcaagt taagggcgcg taaaaattta tgggcctaga gcggcccata
    23881 attttttaaa gagaattctg atttaaaaga agaagaaaat cagcccgaca atgagaacaa
    23941 tatcggcaag caatgggcaa aaaatccctt gccaaagaac cttctgcgga cgaaagccca
    24001 cgccgtgaat cacaccagca cagacggccc acatcagcaa taaaccatgc cagatttcca
    24061 gttcactggt cttcgcggca aaacgagatg ggtcccaaaa catacatcct gctaacagaa
    24121 gcgccatcac gaaggaaagc gcccgcaggg ggcgcttgtc cattaccgca tacaaggtcg
    24181 cgataatctt actcatcaga tgtcttcttg accggctttg cctgattcga cgtgttcaag
    24241 cgccagcgcg gtgattaccc caaacgaaca ggcaagaagc gttcccagaa tccatgcgaa
    24301 ataccacatt tttagctcct tacttagtac agagagtggg tgttacgttc aatatcttct
    24361 ttggtgatac gaccgaacat tttccagtaa caccaggcgg tgtagagcag aatgatcggt
    24421 accagaacca ccgcaaccca ggtcatgacg ttaagcgtca gctggctgga agttgcatcc
    24481 cacattgtca gacttgcgtt catcatggtg ctggacggca tcacaaacgg gaacattgcg
    24541 ataccggctg tcaggatgat gcaggccagc gtcagggagg agaacacaaa cgcccacgcg
    24601 gctttatcca tacgtgcagt caggatggtc agcagcagca gaaccacacc cagtgccgga
    24661 atagcccaca gaattggcgt gttgttgaag ttaaccagcc atgcgccagc ttcacgaacc
    24721 acttctttat tcagtgggtt agaggctgcg taatggtcca ttgtcgattt cacgacataa
    24781 ccatcgatac cgtacatcac ccatacgcca gccagtgcga aacagaccag tgtcaccagc
    24841 gcagccacct gagccgttgc acgggtacgc aggtgcagtt cgcccacggt acgcatttgc
    24901 agataggttg cgccctgagt aatgatcatc cctacgctca ccacgcctgc cagcaggccg
    24961 aacgggttaa gcaactggaa gaagttaccg gtgtagtaca gacgcagata ttcatcaacg
    25021 ttgaacggta cgccctgcaa caggttaccg aacgctacac caattaccag cggcggaacg
    25081 aagctaccaa tgaagatgcc ccagtcccac atgttacgcc agcgggtttc ttcaatcttg
    25141 gagcggtagt caaaaccgac cggacggaag aacaaagacg ccagcacgag gatcatcgcc
    25201 acatagaagc cggagaacgc agcggcatag accatcggcc aggcagcaaa gagtgcgccg
    25261 cccgcggtga tcagccaaac ctggttaccg tcccagtgtg gtgcaatgga gttaatcata
    25321 attcgacgct cggtgtcgtt acgaccgagg aaacgggtga gcatgcccac ccccatgtcg
    25381 aaaccgtcag tgactgcaaa accaatcagc agaacgccaa ccagcagcca ccagataaaa
    25441 cgcaatactt cataatcgat catttgacga ctcctgtctt agcgtgccgg ctgagtagtc
    25501 gtggaagact gctcaaagtg atagcgaccg gttttcaggc tgcttgggcc gaggcgtgca
    25561 aacttgaaca ttaagaacaa ttctgccacc aggaacaggg tatacaggcc gcaaatcagc
    25621 accattgaga agatgagatc gcctgcggtc agtgacgaat tcgccacagc tgtcggcagc
    25681 acttcaccga tagcccacgg ttggcggcca tattcagcca cgaaccagcc cgcttctaca
    25741 gcaatccacg gcagcggaat accgtacagc gcggcgcgca gaagccattt tttctcgcca
    25801 atgcggttgc ggatgacact ccagaaagag agcgcgatga ttgccagcag caggaagcca
    25861 cacgccacca tgatacggaa cgcaaagtac agcggcgcta cacgcgggat ggagtctttg
    25921 gttgcctgtt gaatctgcgc ttcagtcgca tcagccacgt ttggcgtata gcgtttcagc
    25981 agcagaccgt aaccgaggtc tttcttcatg ctattgaact ggtcacgaac cgtctggtcg
    26041 gtagaaccag aacgcagttg ttcgagcaga gagtacgcct tcatcccgtt acgaatgcgt
    26101 tcttcatgct gcaccatcag ctctttcagg ccgataaccg gggtatccac ggaacgcgtt
    26161 gcaatgatgc ccagtgcgta agggatctga atcgcaaatt tgttcgtctc ctcttcctga
    26221 tcaggaatgc cgaacaaagt aaaggcagca ggcgcaggtt gcgtttccca ctcggcttca
    26281 atagcagcca gtttggtttt ctgcacgtcg cccatttcgt agccggattc atcacccaga
    26341 acaataacag acagaacagc agccataccg aagctggcag cgatagcaaa ggagcgttta
    26401 gcgaaggcga agtcacgacc tttcagcata taccatgcgc tgataccgag gatgaacatc
    26461 gcgccagtca cataaccaga cgctacagtg tgaacgaatt tcacctgagc aaccgggtta
    26521 agcaccagct cggagaagct caccatctcc atacgcatag tttcaaagtt gaaatcggac
    26581 gcgattgggt tttgcatcca gccgttcgca accagaatcc acagtgcgga caggtttgaa
    26641 ccgagcgcca ccagccaggt gacacacata tgctgaactt tacccagacg atcccaaccg
    26701 aagaagaaca gacctacaaa ggtggattcg aggaagaagg ccatcagacc ttcgattgcc
    26761 agcggcgcac cgaagatatc ccctacatag tgggaatagt aagaccagtt agtcccgaac
    26821 tggaactcca tggtcagacc ggtagccaca cccagagcga agttgatacc aaacaacttg
    26881 ccccagaact tggtcatatc tttataaatc tgtttgccgg agaggacgta gaccgtttcc
    26941 ataatggcca ggaggaacgc cataccgagc gtcagtggca caaaaaggaa gtggtacatc
    27001 gcggtcaagg caaactgtaa gcgcgacagt tcgactatat ctaacatcat gactccttgc
    27061 tcatcgcatg aagactccga gagtgaaccc cgttagaaag ggtcacacgc atgccccaat
    27121 agaaatttgt ttgaacactt tcatcatacc atgtgaatgc acacaaaaac ggcgtaaaga
    27181 aaggttgcaa acatgttaat aaaaactcaa attgatccca cgtatatatt acgccgcaaa
    27241 atccttacaa taaacaggtt tttgttgaag caaatttgca tttttcgacg gtgatcaatt
    27301 tatagctaaa ttgccgcctt tcagccaatt tgatcgagaa caatttatct ctttttgatg
    27361 cccatttcca agacttatac attgataaat atcaatttct acaaattatg ttaatcatgt
    27421 ttcaacaagg taactttagt gatatttatg tttaaaatgt gtgaattata agactgaaat
    27481 tagtttcggt tggcaattaa agataaaaag agacgaagca aaaacagcac ggttgacctt
    27541 aattcttttt ttggtcactg gctaatacga cgcagagttt caggattatt aataaaggca
    27601 taaatctccc tcccgccgat attaacattt aattaactta taattatatt ataattaact
    27661 cacccgtggt tattattttg ttaaaaataa agccacatat acgtagcttg agagtgaaga
    27721 taaacacaaa attacctgat gcgctacgct tgccagtgtc tacatcatct ctgcaatata
    27781 ttgaatttgc ctgcttttgt agaccgaata aagcgttcat gccgtatccg gcatgaacaa
    27841 agcgcccttt gtcaacaatc tggccgcgca tatgtggctt cggtttgatt gctattcagg
    27901 caagccggta actgaacgtc cgtgactggc cgggtaaaaa aggccccgat agatttgaac
    27961 cttgattttc ctcggtggta atgtgttcat ccatcatcgt ttctgagcaa gaaatcacct
    28021 cgcgactgaa agcaacagtc gcatcacagg ttgctgattc agccggatta aacagccgta
    28081 aaatcacttc ttgtcggtct tcagctttct taagtgcgct tatcaggcat cccactgggg
    28141 gcattttcaa caaactataa ctttccggca cgttgaatcc ggctttgttg agcttcatcg
    28201 catcccatgg gattttgttg taacactgta ctggagtcag ccatgctcgc gcctgctgag
    28261 ctacaccagc ggcggttggc gtaccggtat aactcaataa acttaggcga caagaaagca
    28321 gaccacgtag ttgtgagtcc gggactggca ttttaattcc cgaaggccgc ctaggcctta
    28381 aaagcagatc ttctttcccc agtaagccca cgccgcgcag caacgtaatg gcaaaggttt
    28441 tcttctcttc accgatgact tcaaattcac gtagcccttc gctaaagaca gccatgccgt
    28501 tacgcccttc ctgtaaggct gcatagttga gcatattcca taccggaacc ggcgcttctt
    28561 tccagctttc ttgctgccag ttgttcattg cactgtcgtt cacggggcgc gttagcgaac
    28621 caaactgggt atctgccaga acactatcgg tgttaaaaga tgtagggatc aggacgcgaa
    28681 ggcgatgatc gtcagcctgg ttatcaaggt tgatatccac atcaatgcgc ctgttgttat
    28741 gactaagagt gacaaccaac tcgacatcta ctctgccagt ggattgtctt gcgctgcgtt
    28801 gttgcagatt acgcggcacc gccatttcat agcggataac agccctgctc tgccaggcct
    28861 catgaataat ctcgcattgc ggtttcgcat tcgccgaagt aattacccac tcttcttttg
    28921 cgggtgaata gtcatattca tcaccatcat cagagctttc ttcaatttgc aacacccggt
    28981 catagcgcac accgctatct ttatctacca gttgtagcgt tccatcctca ttgagcgcaa
    29041 tttgccagaa agcattttcc agtatccctt cagcgtcact ttttgccgca atcacgttgc
    29101 caggctgatt cgcttcgata taaagcgtgc gatagcccat agaagggaca atctggttga
    29161 tctgtatatc aaactccata aagggatcgt aattaccgta atgaactatt tgccgatcga
    29221 ttaggcctgg atcgatctca cgggcatggc gaataaaata cggtacaggc tgaccgcgat
    29281 cgtctcgtag attaaactgg ctggcgcgca gccgcacagt ggtgttgatc acttcttccc
    29341 gcggccaggg catcaggtta aacaggacga gtttgtcggc gtcgctctgc ggcatgttgt
    29401 cggcgatttt acgcatatag aaacgcagca gattatccgc catgtcttca gccagttcga
    29461 agcgggcgac gatttcgcga tgaactttgt cacgacagca gcagccgatg ctgtcgtggg
    29521 catgattttt taagatctct ttccacattt tctccagcaa gccgtggtgg tatccaaaac
    29581 ccaacgtcct ggccagcgtt gccagcggtt ccagcagatt aacaatctta ttttcaatac
    29641 gtgcgtgggc aactttgata tccatgcgcg tggaaccgat ggtgcgatgc acgcgcatat
    29701 atttgccatc aataaattcc cctttcaggg ttgccagatt atctcgctgc gcttcgatct
    29761 tctcaaatac ctcttcaaag cggctcatca caaactttcg ttgaggataa atttcgcgca
    29821 gtttctccat cacttcgaag atattttgct gcaatggcat ctgatcatgc ccattcggca
    29881 gcaaaatctc tttggttaca gacgcttttt ccagcacgtc aaaataactg tcgaggcgtt
    29941 tgcgtaatcc gttttcgtcc gcaggtaagt acttaccgat ggcataaccc aacggcatca
    30001 cctgcgccgt cacttcgcta ccgtcactgc tttgccacaa aaactcggtt ttatccgtac
    30061 cgtggcgctc agaacatcca cgccagaaca tggtgcaggt aatgccaaat ccattgtaga
    30121 tatgcggcag ttgcccggac atgccaaagg aatcgggtaa ataacctatt ttcatcggct
    30181 cgccaaacgc gaggcagtca cgcattccgt acatcagatt acggacgatg gattccgcag
    30241 aaacaatcgt ggtatcggtc tgggtatacc aggggccgat aatcaacttg ccggcttcta
    30301 ccagtttctt caagcggtct ttgttttccg gtttcaccgc gaaataatct tcgaggatcg
    30361 ccgtttgccc gtcgagtacg taatatttgt attcgttgtc ctgttccagt cggcacagga
    30421 tctcttccat attattgacc agcagaatgc gtgactcttc ggtggtgaaa taccactccc
    30481 gatcccagtg catatgcggg gtgatgtgaa cgcgagatac cgctttcatc gtcgtttcct
    30541 ttttgtttat ggcattacgc catcagtcag atagttgcca tgcttaaccg cgtgacgccg
    30601 ccagatcagg agaattgcag tcgagattgc agcccccacc aatgccgcac caaaccagcc
    30661 aattgccgcc ataacaccgc ccgcgccatt atcatgaagt aaaaagagcg agaaaatgcc
    30721 agcaccgggt gtcgaaaggc cgatattcat cgcaccgaca atagcgcccg ttaccataga
    30781 gcccagcaca aacgaaccaa taacccgcag cggatcttca atcgccatcg ggatcgcccc
    30841 ttcggtaata cctgccagcc ctaacagcca ggtggatttc ccggtctcaa tttcaaactc
    30901 tttaaacagg cgcggtgcga gcatcgtgga agcggttacg gtaaatgccg aaaccatttt
    30961 gacggaggcg aaaatggcat acgggccgta aacgccgttc gccattgcgc ccaggcagaa
    31021 tgcataagcg gctttattca ctggccctcc aaggtcaaag gaacacataa aaccgagaat
    31081 ggcacccagc aacagcgcgt tacttcctga cagaccgttc agccaggcgg taagcgagtt
    31141 attgatccag gcgacaggtt cccccaccac aaacagcatc agactgcccg ctcccaatgt
    31201 accgagcacc gggtagagat aaaaagtcag gaagccattg aatttactgc taagacgcaa
    31261 gtgatttttc atccagcgta tcaagtaacc ggctatcaat ccgccaacga ccgcgccgag
    31321 aaacccggag ccgatcatgt tggcggcaag tccagccgca aagcctggcg ctaacgccgg
    31381 tttatctgcc agcgaatagg cggtataggc tgccagcacc ggtaccatca aaattccgag
    31441 cagcccgccg cccagtttgc ggtacatcca cagccaggag ttttcttcat taaacagatc
    31501 ttgtagcccg aagatttgcg acagtaatac cgcgaccgcc agcaccgtcc cccccgcgac
    31561 aatcaacggt acggcaaaag agatcccgct caacagtgcc tgtttcagct ccgttttgac
    31621 gcttttcacc ggttgcgtat cttgctgtaa ggtacgcgtc tcatcgccac gctcgagggt
    31681 aagcgattgt tggatcaacg cttctgcatg gcgaatcggc tcggcaacag gcacagaaag
    31741 cgcaggaatg ccgttaaaac gctcactctc cttgatggcg acttcagccg caaaaataca
    31801 ggcagttgca ctattgagtt gatccgccgt taaacgccct tcaataccgt tagcaccttg
    31861 cttttcaacg tagacattca cgccgagttt gcgtccggct ttttccagat actccgcagc
    31921 catgtaggtg tgagcaatac ccgccgggca ggccgttacg caaacgatag ttggcgcgtt
    31981 ggaaaaagag gcagaaggtt gcgtgcctcc cttgtcatcc agcgccgaga gcaattcatc
    32041 aggcgtcgtc gccgactgta tacgcgcccg aatctcatca tccgccaggc gcgtggtcag
    32101 cgctgtcagc agttgcatgt gcgtagtacc cgcttcattg aggggaatcg ccagcaacac
    32161 cactaaatca accgcttccg ggccatccac gccttcccac tgaagcggct cgctgagtgt
    32221 ggcgaccgca aacgccgctt cttttaccgc agcagttttg ccatgcggca cagccaaccc
    32281 ttcacttaag gcggtcgggc caaggctttc acggcgatac acttctttca gaaattgctc
    32341 agtactggaa attttcccca gagcagcaag acgttgagtc aacgcgtgaa tggcctcttc
    32401 acggctggta aagcgcgcat tcagacacaa cgcatcgcgg tgggtcagag tcgtcaggtt
    32461 catcatgatc ctcacttgat cgtttttata gtcgcgccag tgtgcccgac aaaacaatac
    32521 cattgcgtaa tcaatttcac ttgattgaaa ttatttgcat tttatttgta ttgtttacag
    32581 gatgagttta tcgggcataa tggctaaatt cgtattaatg agatgctaaa taatgggaca
    32641 caaaccctta taccgacaga ttgctgatcg cattcgtgag caaattgcgc gtggcgagct
    32701 taaacctggc gatgcgttac ctaccgaatc ggcattacaa acagagtttg gcgtcagccg
    32761 ggttacggtg cgtcaggctt tgcgccagtt agtcgagcaa cagatcctcg aaagcattca
    32821 gggcagcggg acttacgtca aagaagagcg ggtcaattac gatatttttc agttaaccag
    32881 ttttgatgaa aaactgtcgg accgtcacgt cgatacacac agtgaagttc tgatatttga
    32941 agtgattccg gctgacgatt ttcttcagca acagctacaa atcaccgtgc aggatcgtgt
    33001 ttggcatgtg aagcgcgtgc gctatcgcaa gcaaaagcca atggcgctgg aagaaacctg
    33061 gatgccgctt gcgttgttcc cggatctcac ctggcaggtc atggaaaatt cgaaatatca
    33121 ctttattgaa gaagtgaaga agatggttat cgatcgtagc gaacaggaaa tcattcctct
    33181 gatgccaacc gaagagatga gccgtctgct taatatcagc cagacaaagc cgattcttga
    33241 aaaagtatcg cgtggatatt tagttgatgg tcgggtgttt gaatatagcc gcaacgcttt
    33301 taataccgat gactataaat ttaccctgat agcgcagcga aaatcatcgc gataagcaca
    33361 aaaaggccat catacgatgg ccaaccatgt cgaaaccgac agcccttcgc cattcctgta
    33421 gagggaagcg gcgagggcta tttcttatta cagatattta tttcagaaca gttttcagtg
    33481 cttcaccgat atccgccagg ctgcgaacgg ttttcacgcc tgcggcttcc agagcagcga
    33541 atttctcatc cgcagtccct ttcccaccgg caatgatcgc acccgcgtgg cccatacgtt
    33601 tgcctttcgg cgcagtcaca ccagcgatgt aacccacaac tggcttggta acatgctctt
    33661 tgatgtacgc tgccgcttct tcttcagcgc taccgccgat ctcaccgatc atcacgatcg
    33721 cttcggtctg cggatctttt tcgaacattt cgagaatgtc gataaagtta gagccaggga
    33781 tcgggtcacc gccgataccg acacaggtcg actgaccgaa accgtaatcc gtggtctgtt
    33841 taaccgcttc ataggtcagt gtaccggaac gggaaacgat acccacttta cccggtttgt
    33901 gaatgtgacc cggctggata ccaattttgc attcacccgg ggtgataacg cctgggcagt
    33961 tcgggccgat catacgaacg cctgcttcgt ccagtttcac tttcacggtc agcatatcca
    34021 gcgtcgggat gccttcagtg atggtgataa tcagtttgat gcctgcatcg atagcttcca
    34081 gaatggagtc tttgcagaac ggtgctggta cgtaaataac agacgcggaa gcgccagtag
    34141 cagcaacagc ttcacgcacg gtgttgaaca ccggcaggcc gaggtgggta gtgccgcctt
    34201 tacctggagt tacgccacca accattttag tgccgtatgc aatggcctgt tctgagtgga
    34261 aagtcccctg gctaccggta aagccctggc agataacctt ggtgttttta tcaattaaaa
    34321 tggacattat ttcccctcca ctgcggcaac aacctgctga gctgcatccg tcagaccttt
    34381 tgctgcaata atattcaggc cgctgtcagc cagtttcttc gcgccgagtt cggcgttgtt
    34441 accttccaga cgtaccacga ccggtacgtt aacacccact tccgctaccg cgccgatgat
    34501 accgtcagcg atcaggtcgc aacgaacgat accgccgaag atgttaacca gaacggcttt
    34561 cactttgtcg tcagagagga tgattttgaa cgcttcggtt acacgttctt tggttgcgcc
    34621 gccgccaacg tccaggaagt tagccggttc gccgccgtgc agtttaacta tgtccatcgt
    34681 acccatcgcc agacctgcgc cgttaaccat acaaccgatg ttaccgtcca gcgcaacgta
    34741 gttcagttcc cactgtgcag cctgtgcttc acgcggatct tcctgcgact ggtcacgcat
    34801 ttcgcgcaga tcaggctggc ggaacagggc gttgccatca gcgcccagtt tgccgtcgag
    34861 gcaaatcaga tcgccctgtt tggtgatgac cagcgggttg atttcgatca gcgccaggtc
    34921 gcgctccagg aaaatggtcg ccaggcccat gaagattttg gtgaactgct gaaccagttt
    34981 accttccaga cccagtttga acgccagctc gcgtccctga tacggcatcg ggccagtcag
    35041 cggatcaagc gcaactttat ggatcaggtg cggagtttct tccgccactt tttcgatttc
    35101 cacgccgcct tcggtggagg ccataaagac cacgcgacgg gaactacggt ctacaacggc
    35161 accgagatac agctctttag cgatatcggt cgccgcttca accagaatct ggttaaccgg
    35221 ttggccattg gcatctgttt gatacgttac cagacgcttg cccagccagt tttctgcaaa
    35281 agcacggatg tcttctttgc tgtttacaac tttcacaccg cccgctttac cgcggccacc
    35341 agcgtgaacc tgacatttca ctacccacgg accggcaccg atttttgaag cggcttcttc
    35401 tgcttcgcgc ggagtagtac aggcataacc caccggtgct ggtaagccat agcgggcaaa
    35461 cagttgtttt gcctgatatt catgtaagtt catgtgttct gtccatcctt cagtaatcgt
    35521 tatcttttaa accgtagacc gacaccctat ccacgtcggt ctacagtgca ggtgaagctt
    35581 aaactactac acgtccagca gcagacgcgt cggctcttcc agtaactctt tgatcgttac
    35641 caggaagccc acggattcgc gaccatcgat cagacggtga tcgtaggaca gcgccaggta
    35701 catcatcggc aggatctcaa cctgaccatt caccgccatc ggacgatctt tgatagcgtg
    35761 catacccaga attgcgctct gcggcgggtt gatgatcggc gtagacatca gggaaccgaa
    35821 cacaccaccg ttggtgatgg tgaagttacc accggtcaga tcttcaaccg tcagcttgcc
    35881 atcacggcct ttgactgcca gctctttgat tttcttctcg atgtctgcca tgccgagggt
    35941 atcgacatca cgcagaaccg gcgtcaccag gccgcgcggc gtagaaaccg ccatgctgac
    36001 gtcgaaatag ttgtggtaaa ccacgtcatc gccgtcgata gacgcgttca cttccgggta
    36061 acgtttcagg gcttcaacca ccgctttcac gtagaaggac ataaagccca gacggatgcc
    36121 gtggcgtttt tcaaacgctt caccgtactg cttacgcaga tccataatcg gcttcatgtt
    36181 gacttcgttg aacgtggtca gcatggcggt ggagtttttc gcttccagca gacgctctgc
    36241 cacacgctta cgcaggcgag tcatcgggac acgtttttca ctacgtgcag ccagagccgg
    36301 ttgcgccgcc ggagcagccg ctgccggagc agactctttc gccggggctt tcgccagatg
    36361 tttttccaca tcttcacgag tcagacgacc acccacaccg gtgcctttaa tggcgctggc
    36421 gtcgagattg tgttcagcca gcaggcgacg gatcgccggg cttaacgcat cgttgttttg
    36481 ctcttccaga gacgcctgct ggcgttgcgc cggagtggac gctttctctt cagatttggc
    36541 gctggtttct ttaccggcgc tgttgccttc acgcaggcga ccaaggatct gacgagacgt
    36601 taccgttgta ccttcatctt ccagaaccgc atccagaatg ccgtctgctg atgccggtac
    36661 ttccagtacc actttgtcag tttcgatttc taccagcact tcatcacgta cgactgcgtc
    36721 gccgggtttt ttatgccagg ttgcgacggt ggcatcggct acggattcag gcaggtcagg
    36781 gaccagaata tctacgctac tcattgtgta tcctttattt attcgacgtt cagcgcgtca
    36841 ttaaccagat cttgttgctg tttctggtga acggacatat accctaccgc cggagaggcg
    36901 gaggccgggc ggcctgcata acgcagagaa gccccaaacg gaatcacttc acggaaatga
    36961 tgctggctgc agtaccatgc gccctggttg agcggctctt cctggcacca gacaaaatcc
    37021 ttgacgtgag caaactgctg caacacttcc tgcatcgctt tatgcgggaa cgggtagagt
    37081 tgctcgatac gcacaatggc gacatcgtgt tgattgttct tacgacgctg ttccagcagg
    37141 tcgtaataaa ccttaccaga acacatcact acgcgcttca cgcccttcgg atcaagctcg
    37201 tcgatttcac cgatggctgg caggaaggtg ccgttcgcca gttcttcgag gctggaaacc
    37261 gccagcggat gacgcagcag ggatttcggc gacatcacga ccagcggacg acgcatcccg
    37321 cgcagcgcct gacgacgcag catgtggtaa acctgtgccg gggtagacgg tacgcaaacc
    37381 tgcatgtttt gctcagcaca aagttgcaga taacgttcca gacgcgcgga ggagtgctcc
    37441 ggcccctgcc cttcgtaacc gtgcggcagc aacatcacca gaccacacat ccggccccat
    37501 ttctgctcgc cagaggagat gaactggtcg ataaccacct gcgcaccgtt ggcgaagtca
    37561 ccgaactgcg cttcccagat ggtcagggtg cgtggttctg cggtggcata accatattca
    37621 aacgccagca cggcttcttc tgacagtacg gagtcccaga cacggaacgc gccctgaccg
    37681 ttatggatgt gttgcagcgg cgtgtaagtg gaaccgttag actggttgtg gatcaccgcg
    37741 tggcggtgga agaaggtacc gcgaccggag tcttcacccg acaggcgaac cggaatgcct
    37801 tcatcaacca gcgtggcgta agcgaggttt tccgcaccgc cccagtcgaa cagtttctca
    37861 ccggctgcca tcgcctggcg atcgccataa atcttggcaa cgcgagactg catttcaact
    37921 gcttctggta ccgtactgat acgtttagcc agttcctgca ggcgcttcat ctcaactttg
    37981 ttcgggtact cttcgtccca ttcgtggttg aggtacggcg accaggtgaa agagtgcatg
    38041 ttcatcggac gccactctgc cactacgcaa tcgccagcat ccagcgcatc gcggtacagg
    38101 ttaaccatct cggtggcatc ttccagcgtc gccacttttt cctgctccag cttgtcagcg
    38161 tagattttgc gcggtgtcgg atgttttttg attttctgat acatcagtgg ctgggttgcg
    38221 ctcggctcgt cggcttcgtt gtggccgtga cggcggtagc ataccaggtc gatgaagaca
    38281 tcacgtttaa aggtgttacg gaaatcgagc gccagacggg tcacaaaggc aacggcttcc
    38341 ggatcatccg cgttaacgtg gaaaatcggt gcctgaacca tcttaccgat atcagtacag
    38401 tacggtgtag aacgggcatc cagcgggtta gaggtggtga aaccaacctg gttgttgata
    38461 acgatacgta ccgtaccgcc aacttcataa ccacgcgctt tcgacatgtt cagagtttcc
    38521 tgaaccacgc cctgcccggt cactgcggcg tcaccgtgga tggtgattgg cagcactttg
    38581 ttgctgttcg gctcatcaag tctgtccaga cgggcacgaa cagagccgat aacgaccggg
    38641 cttacaatct caaggtgaga cgggttaaac gccagcgcca ggtgcaccag gccgccatcg
    38701 gtctggaagt cagacgagaa gcccatgtgg tatttcacgt cgcctgtgcc gaggtgttct
    38761 ttatgtttac cggcgaactc gtcgaacaag tcttgcggtt ttttacccag cacgttcacc
    38821 agcacgttca gacgaccacg gtgcgccatc ccgagaacca cttcgcgggt gccgctgttg
    38881 ccagcgtggc ggatcatctc tttaagcatc gggattaacg cgtcaccgcc ttccagcgag
    38941 aagcgttttg cgccagggaa ttttgcgccg aggtaacgct caaggccttc agcggcggtc
    39001 agttcgctta agaagcgttt tttctcttcg ctattgaaag tcgcgcgacc agactcgata
    39061 cgctgttgga tccagcgttt ttcttcggtg ctggtgatgt gcatatactc ggcaccaatc
    39121 gggccgcagt aggtttgctt gagggcggaa ataagctcgc cgagtttcat ggtttctttg
    39181 ccgctggcaa atgaaccgac gttgaaggtt tcctggaagt ctgcttcggt cagatcgtgg
    39241 aaagacggat ccagatcggc cactttatct tgctgccaca gtcccagcgg atctagattc
    39301 gcatgctggt gaccacggaa gcggtatgcg ttaatgagct gcaggacttt aacctgcttc
    39361 acattggtgt cagggtcgga gatcgttgaa gagtaacgtg aagcgtcttt cgccaggcgg
    39421 cggaaatatt cacgcgtttg agagtggaat tgatccggtt tgactcccgt accaggtaac
    39481 tgctggaacg tcgaacgcca gttagcgtca accgagtcag gatcggttaa gaagtcttca
    39541 tagagctgtt ctatccagct ctggtttgcg ccagagaggt aagaagagtc caaccaggct
    39601 ttcaaagcgc tgttctgcat cgtgatccct taagcatctt ttttatgctt acttcgccgt
    39661 ggatactacc acgcacagtg atgtgcgtgc cggggttcac ttgcgagctc ttgtctacgt
    39721 agtggctcgc gaaggaacct ttaaaaactg tcttagtgtc aggggcagtt tttaaagatt
    39781 tcctgcattt ctttgttacg cctgatgcgc tgcgcttatc aggcctgcgg gtaatgactt
    39841 gtaggccgga taaggcgttc acgccgcatc cggcactggt tgcctgatgc aacgcttgcg
    39901 cgtcttatca ggcctacagt ttacgcatta cgttgcaaca acatcgactt gatatggccg
    39961 atggcgcgcg tcgggttcag ccccttcgga catacactga cgcagttcat gatgctgtga
    40021 cagcggaata cgctgaatgc atcgctcaaa ccgtcgaggc ggctgtcagt ctcggtatca
    40081 cggctatcga tcaggaaacg atatgccgct aacaagcctg ccgggccgat aaacttatcg
    40141 ggattccacc agaaagacgg acaagaggtt gaacaacatg cgcagagaat acattcatac
    40201 agcccgtcga gtttttcgcg ctgctctggc atctgtaaat gctcgcgagc tggcggattt
    40261 tgtccattat tcaacaggta aggcttaatt ttctcatatt gcgcatagaa ttgtcccatg
    40321 tctaccacca aatcgcggat caccggtaaa cctggcagcg ggcgaatcac aatcttcttg
    40381 cccggctggt tgagtgccga aatcggggta atacaggcca gaccattctt gccgttcatg
    40441 ttcagaccgt cggaaccgca cacaccttca cggcaggagc ggcggaacga caggctggga
    40501 tctttctctt tcagctggat aagcgcatcc agcagcatca tgtcgcgacc ttcttccgct
    40561 tccagggtgt aatcctgcat acgcggagca tcatcaacat ccgggttata gcgataaatt
    40621 gaaaactcga gtctcatttt cctgtctccg cattagtaag tacgaatctt cggcgggaat
    40681 gccgggcgca gtttcggttc catgttgacg cttcggcgcg tcatggattc cgactcaggc
    40741 agatacaggg agtggcacag ccagttttca tcatcacgat ccgggaagtc gaagcggcta
    40801 tgtgcgccac ggctttcggt acggaagttg gcagaaacag ccgttgcata cgccgtttcc
    40861 atcaggttat ccagttccag gcactcaacg cgctgggtgt tgaactcgct ggaagtgtca
    40921 tccagacggg catttttcag acgctcgcgg atcactttca actgctcaag ccctttcgcc
    40981 atcgcatcac cttcacggaa gaccgagaag ttatgctgca tacattcttg cagcgcttta
    41041 cggatcgcca ccggatcttc accgttacga ttattgttcc accggttcag gcgatccaga
    41101 gacgcttcaa catcagactc gctggcatcg tgcagtgcgc cctgctcggc gatagactct
    41161 tgcagatgca gacctgccgc gcgaccaaag accaccaggt ccagcagcga gttgccgccc
    41221 agacggttag cgccgtgtac cgatacacaa gcgatttcac caacggcaaa cagtcccgga
    41281 acaaccacat cttcgccttt ctcattcaca gtcagcgcct gaccggtaac tttggtcgga
    41341 ataccgccca tcatgtagtg acaggttggg ataaccggaa tcggctcttt caccggatcg
    41401 acgtgagcga aggtacggga aagctccagg atacccggca gacgggattc gagaacttct
    41461 ttacccaggt gatcgagttt cagtttcgcg tgtggccccc acggaccatc acagccgcga
    41521 ccttcacgga tttcaatcat gatggaacgc gccaccacat cacggcccgc caggtctttg
    41581 gcgttcggcg cataacgctc cataaagcgt tcgccatgtt tgttcagcag ataaccgcct
    41641 tcaccacggc aaccttcggt gaccagtacg cccgcaccgg caatgccggt cgggtggaat
    41701 tgccacattt ccatatcctg caccggtacg cctgcgcgga tagccatgcc gacaccgtcg
    41761 ccagtgttaa tgtgggcgtt agtggtggac tgataaatac gccctgctcc gccagtcgcc
    41821 agcacggtag cgcgggcttt gaaataaacc acttcaccgg tttcgatgca cagtgcggta
    41881 caacccacca ccgcgccatc ctggtttttc accagatcca gcgcatacca ctcggagaaa
    41941 atggtggtgt ggtttttcag gttctgctga taaagcgtgt gcaacagtgc gtgaccggta
    42001 cggtcagcag ccgccgcagt gcgtgccgcc tgctcgccgc cgaagttttt cgactgaccg
    42061 ccaaacggac gttgatagat acgaccatca tcgagacgcg agaacggcag gcccatgtgt
    42121 tcgagttcca gaatcgcttc cggcccggtt ttacacatat attcaatcgc gtcctggtca
    42181 ccgatatagt ccgacccttt tacggtgtcg tacatatgcc attcccagtt atcttcatgg
    42241 gtattaccca gcgcaacggt aatgccgcct tgcgcagaaa cggtatggga acgggtcggg
    42301 aagactttag agagcagggc acaggtctgg ccgctctggg aaatttgcag cgccgcgcgc
    42361 atacctgcgc caccggcacc aatcacaact gcatcaaatt ctctgactgg caatttcatc
    42421 acacacccca caccacaacg aatccataaa tcacgtaaac caccagtgca acgacaatca
    42481 ccagttgcag catcaggcgc aaggccagcg gtttaacgta gtcggtcaac acctgccaca
    42541 tgccgatcca ggcatgaatc aagatagaaa acagcgccag cagggtgaac actttggtga
    42601 acgcagaggc gaagaaaccg atccagactt catatgtcag ttcgccactg gtggcgaaaa
    42661 aaccgaccat ataaatgatg tagagcgtca ggacgatagc ggtagcgcga acgaggatga
    42721 aatcatgtac gccattgcgt cctaatgcgg aggcgttgct taccatacga ggactcctgc
    42781 gagaagtgaa agcacgacag taataacaaa ggagattttg gcggagcgtt tacccgcttc
    42841 gaatgtttct tccagatagc caaaatccat catcatgtgg cgaataccta cgacgacgtg
    42901 atacgccaga gcggtaagga tgccccacat gataaatttg acgaagaagc tgcccataat
    42961 cgcggaagct tgctcgaaac cttcagggga agagaggctg gtacccagaa gccactgcag
    43021 gatgcccact gcaacaaagg tgatcacacc ggaaacgcga tggagaatgg acgctatcgc
    43081 cgtgatgggg aaccggatgg tctgtaggtc cagattaaca ggtctttgtt ttttcacatt
    43141 tcttatcatg aataacgccc acatgctgtt cttattattc cctggggact acgggcacag
    43201 aggttaactt tctgttacct ggagacgtcg ggatttcctt cctccggtct gcttgcgggt
    43261 cagacagcgt cctttccata actgcgcgtc atgcaaaaca ctgcttccag atgcgaaaac
    43321 gacacgttac aacgctgggt ggctcgggat tgcagggtgt tccggagacc tggcggcagt
    43381 ataggctgtt cacaaaatca ttacaattaa cctacatata gtttgtcggg ttttatcctg
    43441 aacagtgatc caggtcacga taacaacatt tatttaattt ttaatcatct aatttgacaa
    43501 tcattcaaca aagttgttac aaacattacc aggaaaagca tataatgcgt aaaagttatg
    43561 aagtcggtat ttcacctaag attaacttat gtaacagtgt ggaagtattg accaattcat
    43621 tcgggacagt tattagtggt agacaagttt aataattcgg attgctaagt acttgattcg
    43681 ccatttattc gtcatcaatg gattctttac ctgcaagcgc ccagagctct gtacccaggt
    43741 tttcccctct ttcacagagc ggcgagccaa ataacaaacg ggtaaagcca ggttgatgtg
    43801 cgaaggcaaa tttaagttcc ggcagtctta cgcaataagg cgctaaggag accttaaatg
    43861 gctgatacaa aagcaaaact caacctcaac ggggacacag ctgttgaact ggatgtgctg
    43921 aaaggcacgc tgggtcaaga tgttattgat atccgtactc tcggttcaaa aggtgtgttc
    43981 acctttgacc caggcttcac ttcaaccgca tcctgcgaat ctaaaattac ttttattgat
    44041 ggtgatgaag gtattttgct gcaccgcggt ttcccgatcg atcagctggc gaccgattct
    44101 aactacctag aagtttgtta catcctgctg aatggtgaaa aaccgactca ggaacagtat
    44161 gacgaattta aaactacggt gacccgtcat accatgatcc acgagcagat tacccgtctg
    44221 ttccacgctt tccgtcgcga ctcacatcca atggcagtca tgtgtggtat taccggcgcg
    44281 ctggcggcgt tctatcacga ctcgctggat gttaacaatc ctcgtcatcg tgaaattgcc
    44341 gcgttccgcc tgctgtcgaa aatgccgacc atggccgcga tgtgttacaa gtattccatt
    44401 ggtcagccat ttgtttatcc gcgcaacgat ctctcctatg ccggtaactt cctgaatatg
    44461 atgttctcca cgccgtgcga accgtatgaa gttaatccga ttctggaacg tgctatggac
    44521 cgtattctga tcctgcacgc tgaccatgaa cagaacgcct ctacctccac cgtgcgtacc
    44581 gctggctctt cgggggcgaa cccgtttgcc tgtatcgcag caggtattgc ttcactgtgg
    44641 ggacctgcgc acggtggtgc taacgaagcg gcgctgaaaa tgctggaaga aattagctcc
    44701 gttaaacaca ttccggaatt tgttcgtcgt gcgaaagata aaaatgattc tttccgcctg
    44761 atgggcttcg gtcaccgcgt gtacaaaaat tacgacccgc gcgccaccgt aatgcgtgaa
    44821 acctgccatg aagttctgaa agagctgggc accaaagatg acctgctgga agtggctatg
    44881 gagctggaaa acatcgcgct gaacgacccg tactttatcg agaagaaact gtacccgaac
    44941 gtcgatttct actctggtat catcctgaaa gcgatgggta ttccgtcttc catgttcacc
    45001 gtcattttcg caatggcacg taccgttggc tggatcgccc actggagcga aatgcacagt
    45061 tacggtatga agattgcccg tccgcgtcag ctgtatacag gatatgaaaa acgcgacttt
    45121 aaaagcgata tcaagcgtta atggttgatt gctaagttgt aaatatttta acccgccgtt
    45181 caaatggcgg gttaattttt atatgcctaa acacaaaaaa ttgtaaaaat aaaatccact
    45241 atcggacatt catatattct aaaaaaaaat acatcggtat aaattataaa caaatagatg
    45301 atttttaatc atctatcttc ctctcagtta catgaataaa ccaccaaata tagcgcaccc
    45361 ctataattcc caatacttta ttcatcaaac atttaattca taccatcaat taattacttt
    45421 tacatttaat aaaattaata atatttttaa gaaaaactaa aaacattcga caatcaaata
    45481 agatattctt gcaccgatat acttttttct tttctgttaa aggactcagt tatgtttaaa
    45541 ggacaaaaaa cattggccgc actggccgta tctctgctgt tcacagcacc tgtttatgcg
    45601 gctgatgaag gatccggtga aattcacttt aaaggtgaag ttattgaagc accgtgtgaa
    45661 atacatcagg atgatattga taaagaggtt gaactcggtc aggtgaccac cagccacatt
    45721 aatcagtcac atcacagcga tgccgttgct gtcgacctgc tcttagtcaa ctgtgatctg
    45781 gaaaactcca gcaacggttc cggtggcaag atttctaaag ttgcagtcac ctttgatagc
    45841 tcagcgaaaa ccaccggcgc agatccgatt ctcaacaaca ccagcacggg tgaagccacc
    45901 ggcgtcggcg tacgtttaat gaataaagat caaagcaata tcgttttagg caccgctact
    45961 ccagatatcg acctggctcc gacctccagc gaacaaacgc tgaatttctt tgcctggatg
    46021 gaacaaattg atcaggctac acctgtaacg ccaggcgcag ttacggcgaa tgcaacctac
    46081 gtgctggatt ataaataagt attactttgg cggcgctatc gccgcctttc ataaaatatt
    46141 tatcaggtat ggacaccgtg aatatttatc gactctcttt tgtatcctgc ctggtcgtgg
    46201 cgatgccttg cgcattggcg gtcgaattca atctgaatgt tctcgataaa tcaatgcgcg
    46261 accgcattga tatttcatta ttaaaggaaa aaggagtcat tgctcccggt gagtattttg
    46321 ttagcgttgc ggtaaataat aaccaaatca gtaacgagca aaagattaac tggcacaaaa
    46381 atgacgataa aaccattccg tgcatcaatg atttactggt cgataaattt ggcttaaaac
    46441 ctgaagtccg tcagtcgtta ccattgataa atcagtgcgt cgattttagc tcccgacctg
    46501 aaatgctctt caatttcgat caagccaatc agcaactgaa tattagtatt ccgcaagcct
    46561 ggctggcgtg gcactcagaa aactggaccc caccctccac atggaaagaa ggtgtcgccg
    46621 gtgtcctgat ggattacaac ttgtttgcca gcagctaccg cccacaggat ggcagcagca
    46681 gcactaatct taacgcctat ggaacagccg gaatcaacac gggggcatgg cgcttacgca
    46741 gtgattatca gttgaatcat actgatagcg atgataacca tgaacagtca ggcgaaatat
    46801 cacgtaccta tctttttcgc ccgttaccgc aattaggttc aaaattaacc ctcggcgaaa
    46861 cggattttag tcccaatatt ttcgacggtt tttcttatac cggcgcggca ctggcaagtg
    46921 acgatcgaat gttgccatgg gaactacgcg gctacgcccc acaaattagc ggtattgcac
    46981 agaccaatgc cacggtgacg atcagtcaat caggccgcgt catttaccag aaaaaagtcc
    47041 caccaggccc atttatcatt gacgacctta atcagtctgt tcagggcaca ctggatgtca
    47101 aagtgacgga agaagatggt cgggtgaaca atttccaggt ttcagcagca tcgacgccct
    47161 tcctgactcg tcagggacag gttcgctata aactggccgc gggtcagcca cggccctcca
    47221 tgtcacatca aactgaaaat gaaacctttt ttagcaatga agtttcctgg gggatgctgt
    47281 caaacacctc gctgtacagc ggcctgctgc tttctggtga tgactaccat tctgccgcaa
    47341 tgggtattgg gcaaaatatg ctgtggcttg gtgcgctgtc gtttgatgtc acgtgggcca
    47401 gtagccattt tgatactcag caggacgagc ggggcttaag ctaccgtttt aattacagca
    47461 aacaagtgga tgccactaac agcacgattt ccctcgccgc ttatcgtttc tccgatcgtc
    47521 attttcacag ctacgccaac tatctggatc acaaatacaa cgacagcgat gcgcaggacg
    47581 aaaaacagac gatcagctta tctgtgggcc aaccgattac cccactaaac ctcaatcttt
    47641 acgccaacct gctacatcaa acctggtgga atgcagacgc ctccacgacc gccaacatca
    47701 cggcaggttt taatgttgat attggtgact ggagagatat ctctatttct acgtcattca
    47761 atacaaccca ttacgaagat aaagatcgcg acaaccagat ttacctgtcg atttcgctcc
    47821 ccttcggtaa cggtggtcgg gttggctatg acatgcaaaa cagtagccac agcaccacac
    47881 accgcatgtc gtggaacgat acgctggatg aacgtaatag ctggggcatg tctgccggac
    47941 tgcaatccga ccgtcctgac aatggagccc aggtgagcgg taactatcag cacctgagtt
    48001 cagcgggtga gtgggatatt tctggtacct atgccgccaa tgattacagt tccgtcagca
    48061 gcagctggag cggttctttc accgcaaccc aatatggtgc agcgtttcat cgccgcagct
    48121 ccaccaatga accacgcctg atggtcagca ccgatggcgt ggcagatatt ccggttcagg
    48181 gcaatctcga ctacaccaac cattttggca ttgcggtggt gccgttgatt tccagttacc
    48241 agccttccac cgtggcggtg aacatgaatg acttacccga cggcgtaaca gttgcagaaa
    48301 acgttatcaa agaaacgtgg attgaaggcg cgataggtta caaatcactg gcttcccgtt
    48361 ccggtaaaga cgttaacgtc atcattcgca acgccagcgg tcagtttcct cccctcggag
    48421 cggatatccg ccaggatgac agcggcatta gcgtggggat ggttggcgag gaaggacatg
    48481 cctggttaag cggagtcgct gaaaatcaaa agtttaccgt ggtctggggt gatagccagc
    48541 attgctcgct ccatcttcct gaacatatgg aagacaccgc aaatcgcctg attttacctt
    48601 gtcattaatt aaggaaatga aaatgacatt tatgaaagga ctaccccttt tgttgttggt
    48661 tgccagtctg tgcagccacg ctgcactaca acccgatcgc actcgtattg tgtttaacgc
    48721 taatgataaa gctaccagtc tgcgggtgga taatcgtagc gataaactcc cctacctcgc
    48781 ttattcctgg ctcgaaaatg aaaaagggga gaaaagcgat gatcttctgg tcgccctgcc
    48841 gccgatacaa cgtctggaac cgaaagcaac aacacaggta cggatagtaa agcaagcctc
    48901 gaccacaaaa ttaccgggcg atcgcgaaac gctatttttc tacaatatgc gtgaaattcc
    48961 tcccgccccg gaaaaaaaca gcgaccatgc cgttctgcaa gtcgccattc agagtcgtat
    49021 caaggtgttc tggcgaccgg ctgcattacg caagaaagca ggagaaaagg tcgaactgca
    49081 gttacaggtc agccagcagg gcaaccaact gacactgaaa aatcctaccg cgtattatct
    49141 gaccattgcc tacctgggac gaaatgaaaa aggcgttctc cccggcttca aaaccgtgat
    49201 ggttgcgcct tttagtacgg tcaacacaaa caccggaaat tatagcggca gccagtttta
    49261 tctcggttat atggatgatt acggcgcgtt acgcatgacg acgctcaact gcagcggaca
    49321 atgccattta caagccgtgg aggcgaagaa atgagtgctg gcaagggagt gttgctcgtc
    49381 atctgtctgt tatttctgtc gttgaagtct gcgcttgccc tgaactgcta ctttggttca
    49441 tccggtggct cggtagaaaa atcagaagcg attcaacctt ttgctgtacc aggcaatgcc
    49501 aaactcggcg ataagatctg ggaatctgac gatattaaaa taagatctgg gaatctgacg
    49561 atattaaaat tcccgtctat tgtgacaaca ataccaacgg caactttgaa agcgaacatg
    49621 tctacgtatg ggtaaacccc tacccggggg tgcaggatcg ttattatcaa ctcggagtaa
    49681 cttacaatgg cgttgattat gacgccaacc agggaaaaag ccgcatcgac actaaccagt
    49741 gtatcgacag taagaacatt gacatttata cccctgatgg gatggcagaa caaaatttgc
    49801 tcgggtgatc ccgctaatat tcacatgtcg cgaacctttc tcgctcgcat gcggttatac
    49861 gtcaaaatac gagaaatgcc gccgcatgat tatcaaagta cgcttagcga ctatatcgtc
    49921 gtgcaatttg acggtgccgg tagcgttaac gaagacccta ctgcccaaaa cctgaaatat
    49981 catattactg gtctggaaaa cattcgcgtg ctggattgca gcgtcaattt ttccatttca
    50041 ccggaaacac agatgattga ttttggtaaa tttaatttgc tggatattcg tcgccacaca
    50101 atgtcgaaaa cattcagtat taagacgaca aaatcacaaa atgaccaatg caccgacgga
    50161 tttaaagtca gttcctcctt ttataccgaa gaaacgttgg ttgaagaaga taaagcattg
    50221 ttgattggta atggtctgaa gctacgtttg ctggatgaaa acgcctcgcc ttataccttc
    50281 aataaatatg ctgaatacgc cgatttcacc agcgacatgt tggtctacga aaaaacctat
    50341 acagctgaac tttcgtccat tgcaggtacc cccatcgagg ctgggccctt cgatacagtg
    50401 gtgcttttta aaattaacta taactgattt gccatgatgc cctttgcagc cggaaagggc
    50461 atctctttca gaaaattgct gattttcccc ttttccttga ttgctgttat tttttcctag
    50521 cccggctttc tttatgctgt atcgatacac caaagagaaa ataatgaggg agcgaaggat
    50581 gccagttttg cagtggggaa tgttatgtgt gctatcactt ctcctttcta ttggtttcct
    50641 cgcggtacat ctcccggcgg cgctattact tgggccgatg atcgccggga tcatcttcag
    50701 tatgcgcgga attactctgc aacttccccg ctccgctttt ctcgccgcgc aggccattct
    50761 tggctgcatg attgcgcaaa acctcactgg ttcgattctc accacgctgg ccgccaactg
    50821 gccgatcgtg cttgctattt tactggtgac gctgctttcc agcgccatcg tgggttggtt
    50881 attggtgcgc tatagctcac tgcctggaaa taccggtgcc tggggttctt ctccaggcgg
    50941 cgcagcggca atggtcgcta tggcgcaaga ttacggcgca gatattcgcc tggtggcgtt
    51001 tatgcaatat ctgcgagtgc tgttcgtcgc gggggccgct gttctggtca cccgcatgat
    51061 gctgggtgat aacgctgaag cggttaacca gcaaatcgtc tggttcccgc cagtgagcat
    51121 taatctcctg cttacgattt tgctcgcagt cgttgccggt acggcgggat gcatgctgcg
    51181 tcttccttcc ggcacgatgc tcatcccgat gctggcgggc gcagtgctcc agtctggtca
    51241 gctcatcacc atcgaactac cggaatggct gctggcgatg gcgtatatgg caattggctg
    51301 gcggattggt cttggtttcg ataaacaaat cttactgcgg gcactacgcc cgctaccgca
    51361 aatcctgctg tcgatttttg ctctgctggc tatttgtgca ggtatggcgt ggggactgac
    51421 ccggtttatg catattgatt ttatgactgc ctacctcgcc accagtcccg gcgggcttga
    51481 taccgtggcg gtcatcgccg cagggagcaa tgccgatatg gcgcttatta tggcgatgca
    51541 aaccctgcgc ctgttcagta ttttgctgac ggggccagcc attgcgcggt ttatttcaac
    51601 ctatgcgccg aagcggtcgg cctagtgctg gcagcctgga caccagtaaa acgggcgcga
    51661 ggacaacgtg gttttctcaa tgatgccgcc acagcgttcg cacagttcgc catcacgatg
    51721 aaaaacctta aagcgaaaca gcgccccatg atgcttattc tcatccacct gcccccgcgt
    51781 agcgtaagaa aaccgaggaa tctccagtaa cgcgtgtgcg agtgcatcca gttgcgccgc
    51841 attgagatct ttcgctttat gatttccagt caacccaacc tgccagagga tctccacccg
    51901 caaataattg ccaagcccag ccagaaacgc ctgatcgagc agtaatcctg caaactgacg
    51961 gttacgaaag cgcggcgaca ataaccgttc ctttaccacc tccggcgtca gattcggatc
    52021 cagcacatcg ggaccaacgc gttgtaaaaa cggatgcgtg gtcagttgtt ccggcctcaa
    52081 catctcaata tccgaggcgc tataaagcag aatggttttg tcagccgttt gcagttttac
    52141 ccgcaatact cgcgtggtct gcggctcttc gccggtatca accacgcgcc agacgccgta
    52201 aagctgatta tggctgtaga gcgttaaacc gttgggaaaa tgagttaaca acgccttacc
    52261 acgcgtttcc acatgggtaa cgtgttgacc gataagttgt gattgataag tttttaactg
    52321 cgggaaggca aaccagacat cagttagtgg tttgcctttg atcgccgcct ccaggttatc
    52381 cgctgcacgg cggatctccg ggccttcagg catggtgata tccttttttc attctttatt
    52441 aagcggggtt atgctgcgac aacaatcccc tttttggcaa atgtagctcg taatcggcgg
    52501 gcgaacgcca gagcgtgctc gccgtcgcca tgcaggcaga ccgtttgcgc cgcgaccgtt
    52561 gcccattcgc cggtgatact tttgactctg ccgtgttgca ccatctccag cgtttgcgcc
    52621 aacgcctgtt cttcgttttc aatcaacgcg cctggctgac ttcgcggcac cagcgagccg
    52681 tcagcctgat aaccgcgatc ggcaaacact tcctcgcgcg ttgtcaggcc atattgcttg
    52741 cctgcacgaa tcagctcgct tcctgccagc ccaacgagaa ccaatgccgg atcgcaagcg
    52801 tataccgctc tggcgatggc gtctgccagt tgtgcttctt tcgccgcctg gttgtacaac
    52861 atgccgtgcg gtttgacatg acgcattacg ccgccttgcg cacgggcaat cgttgccagc
    52921 gcgccaattt gatacagcgt ctgggcgtaa acggtttccg gcggcagctg catggcgctg
    52981 cgaccaaaat tttccctgtc gggaaaactc gggtgagcgc caatcgcgac accatttttt
    53041 attgcttcac gcacgcaagc ctgcatggtt tgcgcatcgc ctgcatgaaa tccacaagca
    53101 atattggcag aggaaaccag cgttaatagc tctgcgtcgc tggcgcagcc ttcgcccaga
    53161 tcggcgttca ggtcaatttt cattgtgcag ccgccacgct aattgttcaa aataacgttg
    53221 ctgatcttgc cgcgctttca gtgcctcttc cagtgaacac tggacaaaat gaatcggctg
    53281 accgagcgga atttgcgcca gatggtacat atcagcctca atgatacagg caatacgtgg
    53341 ataaccaccg gtggtctgtg cgtcgttcat caacacaatc ggctgcccgt tatgcggcac
    53401 ctgcaccacg cccggtaaca aaccgtgaga taacagttcg cgatcggtga tgcgttttaa
    53461 aatttgcccc tgtaaacgat agcccattcg gttactttgc gagctaagct gccagggcga
    53521 acgccagaat gcatcctgcg aggcgcgatc gaactcatga tattccggcc ccggcaaggc
    53581 gcgaatgcgg ttgccccaca gcagctgttt aacgccctgc gcttccataa aatcacgctt
    53641 cgatttgcca atcgggagtc agtcaccatc cttcagtaaa cggccttcca gcccgccaat
    53701 ccccactttg agatcggtgc tgcatgaccc cattaccggc ggaacatcaa taccacccgc
    53761 gaccgccaga taactgcgca tcccgtgctg cgggcgttta agcgttaaac gctggcctgc
    53821 tttcatcggc aatcgccagc cggtccagac ggcgttatca tccaaccgcg cttcgcaacc
    53881 tgcacccgtc agggcaaacc acccatcagt ttcgaactca actgttaact gaccgagcgt
    53941 gatctccagc gcgggggcat tggcgtcatt acccaccagc aggttagcaa tgcgcagcgc
    54001 aggcatatcc agtgcgccac agtggctgat acccgactgg caaaaaccgt gacgaccgcc
    54061 atcctgcaca gtggtgtaca tgcccgcacg aataatcttc agcatattcc ctccttctgc
    54121 ggtacaaatc gcacgctgtc tcccggacgt aataagatgg gttcgtcacg cgccggatca
    54181 aacaggctga gtgaggtatg accaatcaac tgccagccac ccggcgttgc cagcggataa
    54241 acaccagtct gcggcccgcc gatcccgaca gaacctgccg gaacgagcaa gcgcggttca
    54301 gcgcgccgtg gcgtgtgtag ttgttccggc aaactcccga gatacgggaa gcccggttga
    54361 aaacctaaaa accagaccac gtattccacg gaggagtgca attcaacaac ctgtttttcg
    54421 ctcaacccgc aatacgccgc gaccaccgcc aaatccggtc cgcctgcacc accgtaaacc
    54481 accggaattt caataaagcg agactcaggt tccagggcct cgctctcctc ccaccagcgt
    54541 tgcaaacgct ctatggcatc cagcgccagc gactcaggat tacgcaaaat caccgtgata
    54601 ttgttcatgc cgggaatggc ttcaaccaca ttcggcatat ccaccagacg ctgcgccagt
    54661 cgccagatcc gtttctggct agccagcgtc accggcggtt ccagttccag cactaccgcc
    54721 gtttcaccta tcagataaca acgcgctcgt tgcactttcg atccctctga ttattcgtta
    54781 tgcaggatta ggaatatcaa taaaggtcac atcaagatcg gtattttcat tcagccactc
    54841 gctcaatgcg cgaataccac cgcgttcagt ggcatggtga cctgcagcat aaaaatgcaa
    54901 tccctgctcg cgggctgaat gaatggtctg ttcagaaact tcgccagtaa taaaagcatc
    54961 cacgccaaaa cgcgcggcgc tatcgataaa actttgcccg ccgcccgtgc accaggcgac
    55021 gcgctggacc acctcaggtc cggtatcgcc acaccataac ggcttacgtc ccagacgcgc
    55081 ttcaatccag gaggctagtt ccattcccgg cacaggcatg gtcagctctc cccacggcac
    55141 caacggctca atttcgccca tgaccgtgat ccccagtaac gccgccagct gtgcgttatt
    55201 gcccagctca ggatgtgcgt caagcggcaa atgccagcca tacaggttga tatcattcgc
    55261 cagcaacgtt tttaaacggt tacgcttcat tccgcgaatg accggagact ctcctttcca
    55321 gaagtagcca tgatgcacaa tgaccgcatc cgcgcccaga cgcactgcct catcgagcaa
    55381 agcctggctg gcggtgacac cggtaacaat tttttgcacc gtctctttgc cttccacctg
    55441 caaaccattc ggcgcgtaat cactaatcgc cgcgctgttc agtttttcgt taatcagttg
    55501 ttccagttcg gtgtttttca tcttcgcgcc ttccgtattc tgatagctac accgttaaca
    55561 tagcgttaca tcgcctgttc gtcgatagcc aatcttttga catattaaga aacaaacccg
    55621 acgccaaaag acgcaaattg tttatatcat cacgcttcag ctaatgtata tttatgcaat
    55681 cagacagcat attgcataaa taatcagatc tgtatcaaag ttttaacttt aaggaaacac
    55741 aagcacgccg cgcaactatt acgtgacgtt gccgtcaatt gtggaattat tatgaataaa
    55801 cacgcatcac aaccgcgcgc tatttactat gtcgttgcgc tgcaaatctg ggaatatttt
    55861 agcttttacg gcatgcgtgc cctgctgatt ctctatctca ccaatcaact aaaatacaac
    55921 gatactcacg cctacgagtt atttagcgcc tactgttcgc tggtgtatgt cacgccaatc
    55981 ctcggtggct ttttggcgga taaagttctc ggcaatcgca tggcggtgat gctgggggcg
    56041 ttgttgatgg cgatcggtca tgtggtgctg ggtgccagtg agatccatcc gtcattcctc
    56101 tatctgtccc tggcgattat cgtctgcggc tatggcctgt ttaaatctaa cgtaagctgt
    56161 ctgctcggcg agctgtatga gccaaccgat ccgcgtcgtg atggcggttt ctcgctgatg
    56221 tatgcggcgg gtaacgtggg gtctattatc gcacctatcg cctgtggtta cgcccaggaa
    56281 gagtacagct gggcgatggg ctttggcctg gcggcggttg gaatgatcgc gggtctggtc
    56341 attttcttat gtggcaatcg tcatttcact catacccgcg gcgttaacaa aaaagtactg
    56401 cgtgcgacaa actttctcct gccgaactgg ggatggctgc tggttctgct ggtggcaacg
    56461 cctgcactga ttaccgtgct gttctggaaa gagtggtcgg tatacgcctt aattgtcgcg
    56521 actattattg gcctgggtgt actggcaaaa atttatcgca aagcagaaaa ccagaaacag
    56581 cggaaggagc tggggctgat tgtgacgctc accttcttca gtatgttgtt ctgggccttc
    56641 gcacaacagg gcggtagctc gattagcctt tatatcgacc gcttcgttaa ccgcgatatg
    56701 tttggttata ccgttccgac cgcgatgttc cagtcgatta atgccttcgc agttatgctg
    56761 tgcggtgtgt tcctggcgtg ggtggttaaa gaaagcgtcg caggtaatcg taccgtgcgc
    56821 atctggggaa aatttgctct tggccttggc ctgatgagcg ccggattctg cattctgacc
    56881 ttaagcgccc gctggtccgc aatgtatggt cactcttctc tgccactgat ggtgttaggc
    56941 ctcgcggtga tgggctttgc ggaactgttt atcgacccgg ttgccatgtc gcaaattacg
    57001 cgcattgaaa ttccgggcgt gaccggcgta ttaaccggca tctacatgct gctttctggc
    57061 gcgatcgcga actatctggc gggcgtgatt gccgatcaga catcgcaggc ttcgtttgat
    57121 gcttccgggg cgatcaacta ctccatcaat gcatatattg aagtatttga tcaaattacc
    57181 tggggcgcac tggcgtgtgt aggactggta ctgatgattt ggctgtatca ggcgctgaaa
    57241 ttcagaaacc gcgcgctggc gctggagtca taataggcca gcaccgatgc ctgatgcgac
    57301 gcttgacgcg tcttatcagg cctacgtatt tcctgcaatt tattgaattt gcacaaattt
    57361 gtaggctgga ttaggcgttc acgccgcatc cggcatctgg gctcgattgc ctgacgcgtc
    57421 tgttatttcc ccttccgcgc cgcctcatac gctgccaacg tttgtactct cgcctctttg
    57481 tgctcgacta tcggttgcgg ataatccagc ttcacacctg ctttctgcgc ccacttccac
    57541 ggctcatgca ccgctttccc tggtacatcg cgcagctccg gtaaccatcg acgaataaac
    57601 tcgccctcac ggtcaaattt ctcgccctga gttatcggat tgaaaatacg aaaatacggc
    57661 gcagcatcag tacccgtaga agccgcccac tgccagccac cgttattagc tgccaaatca
    57721 ccatcaatca gctgcgacat gaaatatcgc tcgccttcgc gccagtcgat caacaaatct
    57781 ttcaccagaa aactggctgt aatcatccgt agccggttat gcatccagcc agtgctgtta
    57841 agctgacgca tagcggcatc aataatcggg tatcccgttt tgcctttctg ccaggcctgt
    57901 aaatgtgcgg gattgctttg ccactgcaca cgatccgtcc aggcaataaa tggacgatgt
    57961 ttacacaacg aggggtaata cgtcatcaga tgacggtaga actcgcgcca gatcagctca
    58021 ctaagccaga cactaccggc cccaccgtcc agcgcctgcg gctgttccgc caacaagcga
    58081 tgcaaacact ggcgaggcga taacccgccc gttgccaagc tggcggacaa acggctggtg
    58141 ccttccactg ccggaaaatc tcgttgttgc tcatactctc cagcaccgtt ctcgcaaaac
    58201 tggcgtaatt gcgcaatcgc cgctttttct tccaccggga aatgcgcagt atcgaaagac
    58261 tgacgaggat aattcagcgt aatggatggc gcgggcttta tcgatccgct actacgaact
    58321 tttggtgcag cgacgcactc cggcatccct tcccgcagcc gtttcagcca ggcattctta
    58381 aaaggcgtaa agactttgta catctcgtga ttgccggtca tcaccgcccc cggcggcagg
    58441 atcacgctgt catcaaatcc ttcacacacc acgttacgca gcgctctttc aacgtccaca
    58501 tcccgcgctc gctcattcac ctcatactga tagttataaa aaagatgagt aacgctgttt
    58561 tccgcgcaca cctgtttaac tatttcgaca ctggcgacaa agtcatccac ttcacggaac
    58621 aataaaggaa tacctttttc cgccagcgcg atttgtagcc cattcagttg agcattgata
    58681 agttcagcct gacgcggcga catgttatgc gtctcccact tgcgtggtgt agcgatatac
    58741 agcgccagca cgcgtgcaga cgaattgcgg caggcggcag ccagtgcgag attatcgtgc
    58801 agacgtaaat cctggcgaaa ccaggccaga tgggtagtca taaaactcct gaatggcgca
    58861 agttaatcac acgcggccaa agttcgaaat aacgctgccc tgaaagccag gcgtcaggat
    58921 aaagcgccat atagtgtttg atacgcatca gcggcgcaag aagtggttgc tcgccacggc
    58981 gataactgtc gataagcgca gccagctgct ggcgttctgt ggaatcaata tgcgggcgaa
    59041 aataaccctg aacgtgcatc aaaacgttgg tgtgatcgcg cgggttggcg ggatgagaaa
    59101 gcaaaacaat tacgcgttgg cggtactggt tgtaaaaatc atccagattt tgccactgat
    59161 gaatatcagc cacaaacggg ccaagtttgc ggtactcggg ctgagaatgg gccagaaaga
    59221 cgagtttata gcggctgtga taatcgagaa gcgccccgcg cgttaagcca tgttctttca
    59281 gtcgatgtaa ctcatgaagg gcgaagatac ggattagggt gctgtcatca aatcgttgta
    59341 gattcattac tgctcctgaa cggtatacag gagtaagtgt aggcgttggt aaatgatgtt
    59401 tgtgaagtta atccgctagc agggcaagat tacgaaagcg cgctccccgc aagcaactaa
    59461 tcaaggtagt taatcatggc tgaacacgat attacgccag aagtcactat ttccaaaaca
    59521 cagcgccgct ataaagtcgg ctatgtcaga gcccgccacc aggaccgcaa aaccggtata
    59581 gcccggtatt attcacaaca tcccagtctg caccttaagg gcgactggct gaaagaggcc
    59641 ggatttgata ccggacgggg tgtcacggtg aagatttcgg aggggggcct gaccatcatc
    59701 gctgacagtg acgaggtgca ggaactgcgg gatgggatgt ttagtgtgct gaacgaaagt
    59761 taaatattaa aaaagctggg aagatttaaa accttcctag ctttttattc tgtcttatta
    59821 taatggatac tcatcatctt ccgatgaatt tataaattgc tccaagttta catgaagagt
    59881 acttaaccta gtttttaact cattaagtga agcgataatt tcatattttc caacataatc
    59941 atatgatgga tcgtattcat tatctatgtc agaagctaaa aatgatgata ttcgtagttt
    60001 aaatttagct tcatctttat tacattcatt ggcataaggt gaatgcccta tcatagcata
    60061 ttcatctaat ctaaaaggat aagatggttt taattcatcg atcaatgaaa tttcaatcca
    60121 tgttctatat ccaggcatta atggactaat ttctttgagc aaaacctttc cacttaataa
    60181 tgtattgaga ttgttcatta ttatgtctcc cacgggaaaa atatatgtct aacacttcca
    60241 ccatgttgat gaacttgtaa atgtggtaaa tcaccatcag gatttcctgg cccatgacct
    60301 gacactctat atgaaggttt tgttacagaa ggatctttta tatcatccca atgatatgaa
    60361 cctgcttttg ttttattttc agtaagatat tccattaagt tcttagtgct ggggcctgac
    60421 tcacctgtca tatttttata attatgacct aaatatctat ccataaacaa ttgttcagcg
    60481 tcacttctag acctaacagt aactttggtt tcactactat ctaataaacg attcgctttt
    60541 tggttactaa cagcacatgg agttaatcca agtggatcag catattttag aggattaggt
    60601 gcataactgt agagatttaa accgcctctc aaccctatcg ggtcagttac catgaaccgc
    60661 ccgacatccg gatcatagta cctgtgcaga ttgtagtgca gccccgtttc cctgtcgagg
    60721 tactgtccct ggtagcgcag gttctgctcc accccgccgt ggtccttctc ctgtatcgtg
    60781 ttaccccaga cctgataccc cgtctcccac acgattttcc cgtcgctgtc cgtcatctct
    60841 tccggcgcgc cgttcacatc cgtgtgaaaa tacagtatcc ggttcgggcc ctctttgccc
    60901 gccttatcca cccgcgccag cggttcataa ctcccctggt ccctgtagat gtacaggctg
    60961 cctgttccct ccggcctgct ctcctgcgcc agccgcattc cgtcccataa aaattttgtc
    61021 cggttgtacg gcttaccgtc agggctgatg tgctgttttt ccgtccgcct ccccagtgca
    61081 tcgtacacat accggtatgt cccccgcgct gtcgtcactt ccaccatccg gtgttccgca
    61141 tcataacggt aactctgcgt tccgctccgg ccctcctttt ccaccgcacg gccaaattcg
    61201 tcataacggt agtgcacacc acggtaagac atcagccggt tgaacggcac cacgctgttt
    61261 tctgccgtgg ccctttcccc cgcgcgcctc cccagcaggt ttcccgccgc gtccacctcc
    61321 gtttccggct ggtgccacac cgggtgccac tgcgcctgct ccacccgccc cagcgggtcg
    61381 tgcgtctccg tgatatgccc ctgcaggtca tacacatagc gccatttgcc gccctgaggg
    61441 tcggtcatcc ccagcaacag tcgttcttcg tcgctccagc ggaatgtcgt cacctgtccg
    61501 gcttcgtcct ggtacgccgt cacctgattc tgcgcatccc actggtgata acgtgtgcgc
    61561 cccagtccgt ccgtcacgca cagcctgcgc tgcgccaggt cccacgccag ccggtactgt
    61621 tcaccgctgt ccgtgtggtg ccccgtcacc cgccatgccg cgtcatccgg accttcatac
    61681 cagccccact ggtaataata cccttcgccg cccggctggc ggtgcagcgt catgcagtgg
    61741 ttttcgtcgt aagcaaactc acgcagcagg tgccccgtgc tgtcgtacac gccgttcagg
    61801 tggccggcct cgtcatagct gtaacgacgc agcagtaaag gtgcagtctc agggaaatgc
    61861 tgataaatct gtgtcacccg ccggggatgc gccgccagtt cgtaatacag acggatgcac
    61921 ggacgctgct gttcgccgct gatttccaca atgcggcccc ggtcgtcgta gtacagattc
    61981 agacagttgc tgttacggtc gcccagcatt ttcagccgct ggcgctgcgg gtggtgcggg
    62041 tcagcctcaa acagaaaaca ctgcccgtca tcgctgctga taagccagtg cccctgctca
    62101 ccacgcctta ccgccagccc ttcaccgggg ctgtaaaacc cgccccccgg cagtacggct
    62161 tccatatcca agcgtcgccc catgcccgac acaaaggtca tgcagttttc gtctgcgcta
    62221 cccggtgtgc acacgaggct gacttcatac attacactcc agccctgacc aaacattcct
    62281 tccgtacgtt cgtcacgact gctgtacacg cgctgccagt ccagcggaaa gtggcccggc
    62341 agactgaaat cgaagtcatc gtcatcggcc aggtatttgg ccccggtggc ggcattgacc
    62401 ggatgcggaa gggcactggt ggccttctgc gtcagcatgc tcatcccgac actggcagca
    62461 aaacaggcga ttttagaaag cattttgccc gggcgaagaa acatcagcgc cacggttgcc
    62521 accagggtta tctgcgacct gccgcttctg atatccctga ccaccagtaa cggaccgcca
    62581 atgcgcacat ccggtgacac aaacgccccg ttccccggct cattcaccac cctggcctcg
    62641 caggtacagc gtgccccgct gcgtgtcgcc ggctggctgt tgatacgcac atcccgcgcg
    62701 ccttccgcga tatattccgc tgcgggcggc tcgtcatcgc ctttgaacag attactgatg
    62761 ctgccaataa ccgcattcac tccctgaaag gcggtttcca gcataccgcc cggattcagc
    62821 aggttaaggg ccgttcccag gggggcatcc agaaaggcgg cttttttttg tgcgagaaac
    62881 tgcgtcagtg aggaaggatg tttatggcag tcgattttat cgtcaggggt cggggtggat
    62941 cccggggccg cactggccac agtgggctgc catagttccg acacaaaatg tgtcacgcca
    63001 tccaccattt cagaacgccc ttcttccgta ctgagctggt cgtgcagagc ccgggacatc
    63061 agcacaggcc ctgtcaccgg gttaaccgcc agggctatgg cccccgccat ttccagatat
    63121 tccccggcca catcccggaa cgttctggca tcctcctgac gctgcgcttc ttcctgttct
    63181 gccttcgcct gcgcttcctg cgcctctatg tcctgccgcg atgccgcagt cgccgctgcc
    63241 cgcgcggcag gtttgctgtt gatccatgtg tcaccagagc cggtgaggat gtaaccttca
    63301 atctgaggcg ggaaaagcat atttgcgagg gctgaacagg cttcgctgac cttttctgtc
    63361 acgccggtgt acaccatcgc cacacccgcc acaaaaccgg tcagcacgac ggccgtgcac
    63421 cccatcgtga tccccactga cgtcgccagt aacgaggttg tgccgacaat gagggcatca
    63481 ataacgacat ttgcggccac ctccagcacc gcccccagca catctgccat aaacgacgta
    63541 tgcagcaggg catcaccctg tcttgccgcc cagaatgcct cttccatgtc ccgttccctg
    63601 tatgattaat tcgtcctgac ttcctgccgc atcctgaagc gcagattctt tttaatttca
    63661 gcccagcgcg tcatatctgc cggggtcagc ggtgtggttc tggcgtaggt gaaggccatc
    63721 atgacccgct cacccggcaa tacaaatgcc acctgacact ggtacacctg ctcgttaccc
    63781 cggagaaact ggctctgtat ttccaaaccg cctcgttgag ataatattcc gtcataatgt
    63841 aaatagtccg ttttaataaa ggaattattt attgacaggt tctgtttttt taatcatgaa
    63901 tgtaataccc atcaccgctt tacagcacac tttatctttc cagtcataat cactaataat
    63961 attaagcaat tagcaatatt tacaacgacc agagcggata catagttatg tatttaacaa
    64021 tcccaatata ttcactatgc aaataaaacc actag
//
LOCUS       DAJTQQ010000011        60419 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000011 DAJTQQ010000000
VERSION     DAJTQQ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.011"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..322
                     /locus_tag="OO309_000781"
     CDS             <1..322
                     /locus_tag="OO309_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5846030.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            435..1229
                     /gene="panB"
                     /locus_tag="OO309_000782"
     CDS             435..1229
                     /gene="panB"
                     /locus_tag="OO309_000782"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR5846031.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1241..2092
                     /gene="panC"
                     /locus_tag="OO309_000783"
     CDS             1241..2092
                     /gene="panC"
                     /locus_tag="OO309_000783"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR5846032.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="OO309_000784"
     CDS             complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="OO309_000784"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR5846033.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3342..3722
                     /gene="panD"
                     /locus_tag="OO309_000785"
     CDS             3342..3722
                     /gene="panD"
                     /locus_tag="OO309_000785"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR5846034.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3726..4955)
                     /locus_tag="OO309_000786"
     CDS             complement(3726..4955)
                     /locus_tag="OO309_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5846035.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5019..5459)
                     /locus_tag="OO309_000787"
     CDS             complement(5019..5459)
                     /locus_tag="OO309_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5846036.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5564..6334)
                     /locus_tag="OO309_000788"
     CDS             complement(5564..6334)
                     /locus_tag="OO309_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5846037.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6331..7257)
                     /locus_tag="OO309_000789"
     CDS             complement(6331..7257)
                     /locus_tag="OO309_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5846038.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7366..8028
                     /gene="can"
                     /locus_tag="OO309_000790"
     CDS             7366..8028
                     /gene="can"
                     /locus_tag="OO309_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR5846039.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="OO309_000791"
     CDS             complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="OO309_000791"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR5846040.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8811..11201
                     /gene="gcd"
                     /locus_tag="OO309_000792"
     CDS             8811..11201
                     /gene="gcd"
                     /locus_tag="OO309_000792"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR5846041.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="OO309_000793"
     CDS             complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="OO309_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR5846042.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGVGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMVIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12964..13311
                     /locus_tag="OO309_000794"
     CDS             12964..13311
                     /locus_tag="OO309_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR5846043.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13417..14283
                     /gene="speE"
                     /locus_tag="OO309_000795"
     CDS             13417..14283
                     /gene="speE"
                     /locus_tag="OO309_000795"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR5846044.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14299..15093
                     /gene="speD"
                     /locus_tag="OO309_000796"
     CDS             14299..15093
                     /gene="speD"
                     /locus_tag="OO309_000796"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR5846045.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15131..15493)
                     /locus_tag="OO309_000797"
     CDS             complement(15131..15493)
                     /locus_tag="OO309_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR5846046.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="OO309_000798"
     CDS             complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="OO309_000798"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR5846047.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18620..20473
                     /locus_tag="OO309_000799"
                     /pseudo
     CDS             18620..20473
                     /locus_tag="OO309_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="OO309_000800"
     CDS             complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="OO309_000800"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR5846048.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="OO309_000801"
     CDS             complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="OO309_000801"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR5846049.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="OO309_000802"
     CDS             complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="OO309_000802"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR5846050.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="OO309_000803"
     CDS             complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="OO309_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR5846051.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28317..29690
                     /gene="aroP"
                     /locus_tag="OO309_000804"
     CDS             28317..29690
                     /gene="aroP"
                     /locus_tag="OO309_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR5846052.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="OO309_000805"
     CDS             complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="OO309_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR5846053.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="OO309_000806"
     CDS             complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="OO309_000806"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR5846054.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31223..32116
                     /gene="nadC"
                     /locus_tag="OO309_000807"
     CDS             31223..32116
                     /gene="nadC"
                     /locus_tag="OO309_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR5846055.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIVSGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32319..32759
                     /gene="ppdD"
                     /locus_tag="OO309_000808"
     CDS             32319..32759
                     /gene="ppdD"
                     /locus_tag="OO309_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR5846056.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32769..33916
                     /gene="tadA"
                     /locus_tag="OO309_000809"
                     /pseudo
     CDS             32769..33916
                     /gene="tadA"
                     /locus_tag="OO309_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33906..35108
                     /gene="hofC"
                     /locus_tag="OO309_000810"
     CDS             33906..35108
                     /gene="hofC"
                     /locus_tag="OO309_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR5846057.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="OO309_000811"
     CDS             complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="OO309_000811"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR5846058.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36348..36386)
                     /locus_tag="OO309_000812"
     CDS             complement(36348..36386)
                     /locus_tag="OO309_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846059.1"
                     /translation="MIIKTLKMSARK"
     gene            36411..37031
                     /gene="coaE"
                     /locus_tag="OO309_000813"
     CDS             36411..37031
                     /gene="coaE"
                     /locus_tag="OO309_000813"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR5846060.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37031..37774
                     /gene="zapD"
                     /locus_tag="OO309_000814"
     CDS             37031..37774
                     /gene="zapD"
                     /locus_tag="OO309_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR5846061.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37784..37981
                     /gene="yacG"
                     /locus_tag="OO309_000815"
     CDS             37784..37981
                     /gene="yacG"
                     /locus_tag="OO309_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR5846062.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="OO309_000816"
     CDS             complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="OO309_000816"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR5846063.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGGPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38530..41235)
                     /gene="secA"
                     /locus_tag="OO309_000817"
     CDS             complement(38530..41235)
                     /gene="secA"
                     /locus_tag="OO309_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR5846064.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41297..41809)
                     /gene="secM"
                     /locus_tag="OO309_000818"
     CDS             complement(41297..41809)
                     /gene="secM"
                     /locus_tag="OO309_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR5846065.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="OO309_000819"
     CDS             complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="OO309_000819"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR5846066.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="OO309_000820"
     CDS             complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="OO309_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR5846067.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="OO309_000821"
     CDS             complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="OO309_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR5846068.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="OO309_000822"
     CDS             complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="OO309_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR5846069.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="OO309_000823"
     CDS             complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="OO309_000823"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5846070.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47274..48749)
                     /gene="murC"
                     /locus_tag="OO309_000824"
     CDS             complement(47274..48749)
                     /gene="murC"
                     /locus_tag="OO309_000824"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR5846071.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48803..49870)
                     /gene="murG"
                     /locus_tag="OO309_000825"
     CDS             complement(48803..49870)
                     /gene="murG"
                     /locus_tag="OO309_000825"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR5846072.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="OO309_000826"
     CDS             complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="OO309_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR5846073.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51111..52427)
                     /gene="murD"
                     /locus_tag="OO309_000827"
     CDS             complement(51111..52427)
                     /gene="murD"
                     /locus_tag="OO309_000827"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR5846074.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="OO309_000828"
     CDS             complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="OO309_000828"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR5846075.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53506..54864)
                     /gene="murF"
                     /locus_tag="OO309_000829"
     CDS             complement(53506..54864)
                     /gene="murF"
                     /locus_tag="OO309_000829"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR5846076.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54861..56348)
                     /gene="murE"
                     /locus_tag="OO309_000830"
     CDS             complement(54861..56348)
                     /gene="murE"
                     /locus_tag="OO309_000830"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR5846077.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="OO309_000831"
     CDS             complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="OO309_000831"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR5846078.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="OO309_000832"
     CDS             complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="OO309_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR5846079.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="OO309_000833"
     CDS             complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="OO309_000833"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR5846080.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="OO309_000834"
     CDS             complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="OO309_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR5846081.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14475 a        16732 c        14846 g        14366 t
ORIGIN      
        1 aaaggttggt actgaaaacg ggcaactcct tggtaatact ctcacaggta atgatgcagc
       61 aaaaggagtc ggtgtactca tagaaggttt agcaactagt aaaaatcctc taatgacatt
      121 gaaacctaat gattcaaatt ctgtttataa agattacgac ccgagaggca aagacgatac
      181 aacaggagga gtttacccgg atcaagatac aggtataaca taccctctcc atttccaggc
      241 cacgctacaa caggatggaa ctataccaat agaagctggt gagtttaaag ccaccagtac
      301 tttccaggta acctaccctt aataagtcca ccgcaccgcc atcatctggc ggtgcggtaa
      361 ttgataaatc attgccccca ccaatgtgac tgataaaatg ccaggcaaca cgacatcatt
      421 tatcaggata cgttatgaaa ccgaccacca ttgcttcact tcagaaatat aagcaggata
      481 aaaagcgttt cgcgaccatc accgcttacg actacagctt cgcgaaacta tttgctgatg
      541 aagggcttaa cgtcatgctg gtaggcgact cgctgggcat gaccgttcag ggccacgatt
      601 ccactctgcc agtaaccgtt gctgatatcg cctaccacac tgccgccgta cgtcgcggcg
      661 caccaaactg cctgctattg gctgacctgc cgtttatggc gtatgccacg ccggaacaag
      721 cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa tatggtcaaa attgagggcg
      781 gtgagtggct ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct gtatgtggtc
      841 acttaggttt aaccccacag tcagtgaata ttttcggtgg ctacaaagta caggggcgcg
      901 gcaatgaagc gagcgatcga ctactcagcg atgcattagc cttagaagcc gcgggggcac
      961 agctgctggt gctggaatgc gtgccggttg agctggcaaa acgtattacc gaagcactgg
     1021 cgatcccggt tattggtatt ggtgcaggca acgtcactga cgggcaaatc ctcgtgatgc
     1081 acgacgcctt tggtattacc ggcggtcaca ttcctaaatt cgctaaaaat ttcctcgccg
     1141 aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt
     1201 atccgggcga agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct
     1261 gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg tggcgctggt
     1321 gcctactatg ggtaatctgc acaatggcca tatgaagctg gtcgacgaag ccaaagcccg
     1381 cgccgatgtg gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggaaga
     1441 tctggcacgt tatccacgga ccttgcagga ggactgcgag aagctaaaca aacgtaaagt
     1501 ggatttagtt ttcgcccctt cggtaaaaga gatctacccg aacggtactg aaacccacac
     1561 ttacgttgac gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt
     1621 tcgcggcgtt tcgactattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg
     1681 cttcggtgaa aaagattttc agcaactggc gttgatccgc aaaatggttg ccgatatggg
     1741 cttcgatatt gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctaag
     1801 ttcccgtaac ggttatctga cggcggaaca acgcaaaatt gcgcctggtc tgtacaaagt
     1861 tttaagttcg attgctgaca aattgcaggc tggggaacgg gatctcgatg aaattattac
     1921 cattgcgggg caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga
     1981 tgccgacaca ttgctggaag tttctgaaac cagcaaacgg gcagtaattc tggtagccgc
     2041 ctggcttggc gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttaac
     2101 tggcgctacg gctgatggcg ccagttatta atttacccca cgtcatctgt cataacacag
     2161 gtaacttaaa taaggttaat taccttatca atctcagcaa gaggtaaatt tgccatctct
     2221 gcaacgtctt cccgagacat tcctttactt agaagacgca gggcgaattc ttgcctttcc
     2281 tcctgtcttc cctgctgaat ccccttctca atccctttct cttcaaacca ctgcgccagc
     2341 gtcatcatag acttccctcc cgtttccctg tctctcagca caccataaaa caaatccgct
     2401 tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg acttccgcta
     2461 gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca ataacattaa gtcgcgctgg
     2521 cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca
     2581 tccggtgtga tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgcgccagc
     2641 tccggcgagt aaaacatatc aaaccagcac attgagagcg gataaggtgt ggcctcgccc
     2701 tgataaaaca gtatcggcac caccagcggc agcttatcgt ggccagcctc cagatgccgg
     2761 tgcatggcag caatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt
     2821 tggtgttcaa tcacaacatg cagataaccg ggattgccct gcatttgcat ggaatagagc
     2881 acgtccgtgc tgtgaccttt caggcactct tcaatgaaac tccccgactc taaatgaagc
     2941 gtgttgagat cacaaagttc gcgtaattcc actggcaaat gtatctccag aaagtcgcga
     3001 gccgtctccg catgcattaa aaattgttta aataccgcgt catgcggtgt ggtactcggt
     3061 gcatccatct tccctgtctt ccgtgtggcc ttgcgtgaag gcagtatcca gtcaaggcgt
     3121 gctatcgtca gccacctctt tattgtttac cgagcagcgt tcataaaaat atcaattcgt
     3181 tacaggcgtt acatggcacg cttcggcgcg gtacgcaaac tgctgacgca aatgccatca
     3241 gatgagtaga cactcatcaa aaatcgggca atactgcgtg agaatttcct caaagcaggc
     3301 accgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc
     3361 agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg
     3421 ccattgacca ggattttctt gacgcagccg gtattctcga aaacgaagcc atcgatatct
     3481 ggaatgtcac caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga
     3541 gaattatttc tgttaacggt gcggcggccc actgcgccag cgtcggcgat attgtcatca
     3601 tcgccagctt cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aatgtcgcct
     3661 attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt
     3721 gattgttatc cctgcggctg gttactcacc agccgcgaca tcttctccag cgaatccgtt
     3781 cttaagatat aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc
     3841 atggttgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc tttgtcatta
     3901 aagccgccaa acggatacga aagataccag acatgtggat taaattgcgc cagagcgcgg
     3961 cgtgaacgtg caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtcgg
     4021 cgataaccat ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa aacatcgcga
     4081 atttcgttaa gctcagaaac gctcataaat tgcagcgatt ttgggttcca cttctgcggg
     4141 tgacgtttga tgcgtgaggt aacaataaac accgtcgcct tcatgccata ttgtttcaac
     4201 acaggatacg catagcggct caccgacttg aggccatcat caaaggtaat caccaccgct
     4261 cgcgcaggga gattgatcgt attcttcacg taaccttcca gctgagccat gctcagtgtc
     4321 gcgtatcccc tgtcgcgcag ccaggccatt tggttattga aagcgcgtac cgaggtggtc
     4381 gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg ataagttagc
     4441 accggcaggc cattatcggg ttgggcatcc agcgcgctga tataggccag tcgatcgcca
     4501 atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag
     4561 cgcaaattgt ccgccagtac cccaaatggc gcactgccca cactcggcgc gttgtagacc
     4621 ggcgtatctt tccaggtaat taagttctga ttactcagcg gcttgttgag gtcacccaaa
     4681 ccgtcttcaa ctttttgtcg tccctgaact ggctcgagat gccctttatc gataaaccct
     4741 ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt
     4801 tgtccggccc gaatattccc cacggtcacc atcttgtcac taatttgcgc ccagaccgca
     4861 gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac
     4921 agcatcagca ggagaataac agcttgttta tacatgaaaa aataaccgag ggtgaaacaa
     4981 cgcttttatt gtagcaaaag cgccatcaga atcgcgggtt aatactcata caaattatgt
     5041 ttgaggacga aaggcggatt tttttgttgt tggtgccaaa gactactcac ctccggccca
     5101 cccagttcga caatacactc acgaaactct gaactggtca tggtttcacg gcaagccatc
     5161 atctgttcaa ttaacggtaa cgtgacacca gaaatcactt cgcaacggga gtgtttgtgg
     5221 cttaataatg aagccacgcg atacggtggc gctccggcta tatcggttaa gaagatgaca
     5281 ccctcaccag agtccgcttc atgcagagca tcgcacatca tgcggctgag catattagag
     5341 cttaatccgc gccagaaatt cacggcccgg cactgaagaa gtgccccatg ttttttctcc
     5401 agcgcatcca gtatctcttg cgcccggtca tcgtgacagg taattaccca acctaacatc
     5461 gccttcctcc ttagccgaaa atcagcctac cctgaccatt gttgataaag ggtgatagcc
     5521 gtcaaaactc ctctcccccg aatccagagg agaggaaata gccttagcta cgcaaaccac
     5581 gtccacgttg gatcagcgac caacagatca aataaaacgc cacaataaag accaccagta
     5641 cgccaaaggt agtgaccagc ggcacatcat ttataccgag gaagccgtag cggaaaccac
     5701 tgatcatata aacgattggg ttcaggtgcg acagcccttg ccagaacggc ggcaacaaag
     5761 tcagtgagta aaagaccccg cccaaatacg tgagtggcgt taacacaaag gttggcacca
     5821 ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag caaacccgca agggagaata
     5881 gcaccgccgt gagcaccagg gttaaggcaa cgaataccca cgaatgcacc tgaaacggca
     5941 caaaaaacag tgaaattgcc gtcacaagaa tgccaacaaa cagaccacgc gccacgccac
     6001 cgccgacata tccggcaata atgacgtgag tcggaaccgg cgctaccagc agctcttcaa
     6061 tattacgctg gaacttggca ccaaaaaatg atgacgcgac gttagcgtag gcgttggtga
     6121 tcaccgacat catgatcagc cccggtacga tgaactgcat atagctgaag ccatgcatat
     6181 cgccaatacg cgaacctatc aggttaccga agataataaa gtaaagggtc atggtgatga
     6241 ctggcggcac cagcgtctgc acccagatac gcataaagcg atggatctct ttcgcccaga
     6301 tgctttttag cgccacccag taaagatgca tcatgcgcga tctccttgtt tttcattaac
     6361 cagtgaaaca aacagctctt ccagacggtt agctttgtta cgcatactta atacctgaat
     6421 gccctgctca cttaactgcg taaatacgct gttgatcccc tgctcacgta gcacttcaac
     6481 ttccagcgtc gcggtatcga ccagtcgata ctgatagcca tcgagcttcg gtaacggact
     6541 tttcggtgcg agatcgagaa taaaggtttc cgatttcagc ttcgccagca gcgccttcat
     6601 cgaggtattt tccaccagct caccatgttg aataatgccg atattgcggc acagcatttc
     6661 tgcttcttcc aggtagtgtg tggtaagaat gatggtggtg cctttgtcgt ttaaatcctt
     6721 caaaaagccc cacattgagc ggcgaagttc aatatccacg cctgcggtcg gttcgtcgag
     6781 aatcagtagt ttaggttcat gcattaacgc acgggcaatc attaaacggc gcttcatccc
     6841 gccagataac atacgcgcac gttcgttgcg ttttccccat aaatcgagct gcttaagata
     6901 tttttcgctg cggatgtacg cctctttgcg ctccacaccg tagtaccctg cctgattcac
     6961 cacaatttgc tgcacggttt caaacgggtt gaagttaaat tcctgcggca ccagtcccaa
     7021 ctgacgttta gcgttcacga catctttctc gagatcgtaa ccaaatacgc tgacccgccc
     7081 ggaggtttta tttaccagag agctgataat accgatagtg gtcgatttcc cggccccgtt
     7141 cggcccgaga agcgcataaa aatcacccgc ttcgacctgc aaatctattc cacgaagcgc
     7201 ctgaacgccg cctggatagt tttttttaag ctgttgaagt tccagtgcaa tggtcataaa
     7261 tttttactta ccttacgttc ttacacttta tctgtggttt aaatcgtccc ggagttgccc
     7321 tatattagcc aaacgtaatt atttggttac aggtcgttaa cctccatgaa agacatagat
     7381 acactcatca gcaacaatgc actatggtca aaaatgctgg tggaagagga tcccgggttt
     7441 tttgagaaac tggcacaagc gcaaaaaccg cgctttctat ggattggatg ttccgacagt
     7501 cgcgttcctg cagaacgttt aaccggtctt gagccgggcg aactctttgt tcaccgtaat
     7561 gttgctaacc tagtcattca caccgacctg aactgccttt ccgtggttca gtatgcagtg
     7621 gatgtactcg aagttgaaca cattattatc tgtggccact acggttgcgg cggcgtacaa
     7681 gccgccgttg aaaacccgga actggggctt atcaacaact ggctgctgca tatccgcgat
     7741 atctggttca aacatagctc attgctcggc gaaatgccgc aagagcgccg tctggatacc
     7801 ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg gccactccac cattatgcaa
     7861 tcagcgtgga aacgcgggca gaaagttacc attcacggct gggcctacgg cattcacgac
     7921 ggcttgctgc gtgatctgga tgttaccgcc accaaccgcg aaacccttga gcaacgttac
     7981 cgtcacggga tttccaacct caagctgaaa cacgccaacc acaaataaaa atgccatgcc
     8041 ggatgcaaca catccggcaa cttcacactt actcgtccag cagaatcact ttgccaatat
     8101 acggcagatg acggtaacgc tgtgcgtaat caatgccgta acccaccaca aactcatccg
     8161 ggatcgagaa accgataaat tcaaccggga cgttcacttc acgacgggac ggtttatcca
     8221 gcagcgtaca aatcgccaac gacttcggtt cgcgcaggct taagatctca cgcactttcg
     8281 acagtgtatt ccccgagtcg atgatatctt caacaatcag cacgtccttg ccacggatat
     8341 cttcatccag atctttgagg attttcacat cacgggtggt ggacatgccg ctaccgtagc
     8401 tggaggcggt cataaagtcg acttcatgag atacctgaac ttcacggcac aggtccgcca
     8461 taaacataaa tgagccacgc agcagaccca ccagcaccat atcgctgccg ctgtctttgt
     8521 aacgctcagt aatctgacga cccagttcgg cgatacgcgc tttaatctcc gcttcgggga
     8581 tcattacttc tacagtatgt ttcatatctc taaccatatg atttaaaaac aaatcacttc
     8641 gcacgggcag gttttggcac cgcaacaaag cgcaagacgc gcagtatacc agtaaaacta
     8701 ttcattcgcg gaatctgtta ataaagctcg aattgtgatg acggtcacac atgttaaaac
     8761 ctataattaa ttgctactaa aatattaatg aattgaaatg gtgtctcttt atggcaatta
     8821 acaatacagc ctcgcgacga ttactcgtca cgctaacagc cctttttgca gcgctttgcg
     8881 ggctgtatct actcattggc ggaggctggc tggtcgcgat tggcggctcc tggtactacc
     8941 ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt aaacgcgccg
     9001 cgctttggct atacgcagcc ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg
     9061 gtttcgactt ctgggcgctg actccgcgca gcgatattct ggtcttcttc ggcatctggc
     9121 tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac
     9181 tggtggtcgc actgctgatt agcggtggta tcctgacctg ggccggattt aacgatccgc
     9241 aggagatcaa cggcacctta agcgccgatg ccacacctgc cgaagctatc tcccccgttg
     9301 ccgatcagga ctggcctgcg tatggccgca atcaggaagg tcaacgcttt tcaccactga
     9361 agcaaattca cgccgataac gtccacaagc tgaaagaagc ctgggtgttc cgtactggcg
     9421 atgtgaagca gccgaacgat ccgggtgaaa tcaccaatga agtgacgccg attaaagtgg
     9481 gcgataccct ttacctgtgt accgctcacc agcgcctgtt tgcgcttgat gccgccagcg
     9541 gcaaagagaa atggcattac gatcctgagc tgaaaaccaa cgagtctttc cagcacgtaa
     9601 cctgccgtgg cgtctcttat catgaagcca aagcagaaac ggcttcgccg gaagtgatgg
     9661 cggattgccc gcgtcgtatc attcttccag tgaatgatgg tcgcctgatt gcgattaacg
     9721 ctgaaaacgg caaactgtgc gaaaccttcg ccaataaagg cgtgctcaat ctgcaaagca
     9781 atatgccaga caccaaaccg ggtctgtatg agccgacttc tccgccaatt atcaccgata
     9841 aaaccatcgt gatggccggt tcagtcaccg ataacttctc aacccgcgaa acgtctggcg
     9901 tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg ggcttttgat ccgggcgcga
     9961 aagatccgaa cgcaatcccg tcagacgaac acacctttac ctttaactca ccaaactcgt
    10021 gggcaccagc ggcctatgac gcgaagctgg atctggtcta tctgccgatg ggcgtgacca
    10081 cgccggatat ctggggcggt aaccgcacac cggaacagga acgttatgcc agctcgattc
    10141 tggcgctgaa tgccactacc gggaaactgg cgtggagcta ccagaccgtt caccacgacc
    10201 tgtgggacat ggatcttccg gcacagccga cgctggcgga catcaccgtt aatggtcaga
    10261 aagtgccgat tatttacgct ccggcgaaaa ccggtaacat ttttgtgctc gatcgtcgta
    10321 atggcgaact ggtggttccg gccccggaaa aaccggttcc ccaaggtgct gccaaaggcg
    10381 attacgttac ccctactcaa ccattctcgg aactgagctt ccgtccgacg aaagatttga
    10441 gcggtgcgga tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc
    10501 accagatgcg ctatgaaggc attttcacgc cgccatctga acagggtacg ctggtcttcc
    10561 cgggtaacct ggggatgttc gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg
    10621 caattgccaa cccaatggca ctgccgtttg tttcgaaact gctcccgcgt ggtcctggca
    10681 acccgatgga gcagccgaaa gatgccaaag gcacgggtac ggaatccggc attcagccac
    10741 agtacggtgt accgtatggt gtcacgctca acccgttcct ctcaccattt ggtctgccat
    10801 gtaaacagcc agcatggggt tatatctcgg cgctggatct gaaaactaat gaagtggtgt
    10861 ggaagaaacg tattggtacg ccgcaggaca gtatgccgtt cccgatgccg gttccggtgc
    10921 cgttcaatat gggtatgccg atgctgggcg ggccaatctc cacggcgggt aacgtgctgt
    10981 ttatcgccgc tacggcagat aactacctgc gcgcttacaa catgagcaac ggtgaaaaac
    11041 tgtggcaggg tcgtttaccg gcgggtggtc aggcaacacc gatgacctat gaagtgaatg
    11101 gcaagcagta cgtggtgatc tccgcaggcg gtcacggttc atttggtacg aagatgggcg
    11161 actatattgt ggcttatgcc ctgccggatg atgtgaaata accataatgc ccggagagat
    11221 ccgggcatat ttccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc
    11281 ttcatgctcc agcagatggc agtgcgccat ataagcatgt tctttcggtg cgttgtgatt
    11341 aaacttcacc agcacttcac tgacattgcc ttctacctta acggtatctt tccagcccgc
    11401 gcgatgagcc gctggcggtt tgccattttc tgacaagata cggaactgcg tgccgtgaat
    11461 atggaacgga tgcagcatca tgtcgcccac gccagagata acccaacgtt cgtactgccc
    11521 tttcgccgcc gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc
    11581 atggtggaaa tcgaacttcc cgccgtggtt catatgattc atattgccgt gccccatatg
    11641 gcccatcatc tggctgtgat ccattccggc catcgcctga tcgccatatt tctccattag
    11701 catctgcatc cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt
    11761 cagcccttcc agcgaaggta acgcaggcag gctacttaat gtgtcaggca aagcaccgga
    11821 ggcactaata gcaatcggct gaatccgcat taccggatgc ggcttgtcaa acggtgcaat
    11881 caccatcccc atctggctga ccggcagcgt caccaggtca aagggtttgt tgtcgttaac
    11941 ctccaccagc acttcaaaac gctcgcccat cagcaccggc agctcgctca ccttcaccgg
    12001 ttcaggtagc agaccaccgt cgctggcaat cacatacagc gggcgattgt cgctggtggc
    12061 gaaattgagc gagcgggcat tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg
    12121 ggcagcgtgt tgtgggtaga ttgcaccgtt ggtcagcagc gtatcgccaa accagcccac
    12181 ggcggcggtc atcacatcca gttgataatc aatctgcccg tcggcgctaa atttcttatc
    12241 ctgaacgatc accggaacat catcgatacc ccactgtttt ggcagcatta atttcaggat
    12301 ctcgtcatct tcaatcacca ccagaccagc cagccccatc gccacctgtc gcccggtctt
    12361 gccatgttga tgtggatgga accagcaggt agcggcaggt tgatcaacgt tcaacgtcac
    12421 cgagcgcttg ccacctggcg gaataattcc ctgtgggccg ccgtcgacgt cacccggtac
    12481 ttccagcccg tgccagtgca acgtcgtctc ttccgccagt tggttgtaga tatcaaccgt
    12541 taccgctttg ccgcgctgta atttcaccgc cggccccagc agattgccgt tatagcccca
    12601 ggtagttgca gttttctcgc caaaggtgga ctgacctacg ccaatagtta actgaatgcg
    12661 attacgggca tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa
    12721 gactgcacgg ctccacagtg gcaaggctga agccacaccc agtgcgacgg aatatttcaa
    12781 gaaatcacga cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg
    12841 ttgagcatag tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat
    12901 aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa
    12961 gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc tgatcgccgt ttgcgcaaac
    13021 agttacgcgc tcagcgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc
    13081 tttctgaaga acgattgtgg ttaccagaac ttatctaacg ggcaaattcg tcgcgcactg
    13141 gtctttttcg cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa
    13201 gctctcggtg aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaaa
    13261 tgcaaagccc tggcccgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata
    13321 cctttttgta gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc
    13381 tttttttacg ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat
    13441 gaaacgctac acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa
    13501 aagaccgatc accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg
    13561 ctggatggcg tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgacc
    13621 catgttccgc tactggccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac
    13681 ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa
    13741 atcgatgcgg gtgtcgtgtc gttctgccgt cagtatctgc ccaaccataa cgccggtagc
    13801 tacgacgatc cgcgctttca actagtgatc gacgatggcg tcaatttcgt taatcaaacc
    13861 agccagacct ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagc
    13921 ctgttcacct cagcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc
    13981 gtcgcacaaa acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa
    14041 ctcagccatt acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc
    14101 ggtatcatga cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa
    14161 attattcagg cgcgttttct cgcttctggc ctgaaatgcc gttattacaa tccggcaatc
    14221 catacggcag cttttgcctt acctcagtat ctgcaagacg cactggcttc acagccgtcc
    14281 taaggagaag ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa
    14341 agtctgagtt tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt
    14401 tatattgctt atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa
    14461 acctgttcca ttatcggggc caatattctt aacatcgccc gccaggatta cgaaccacag
    14521 ggtgccagcg tcactattct ggtgagtgaa gaaccggttg acccgaaact catcgacaaa
    14581 acagaacacc ccggcccact gccagaaacg gtggttgctc atctcgataa aagtcatatt
    14641 tgcgtacata cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat
    14701 attgaagtct ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac
    14761 cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc gcggttttac tcgcgacatt
    14821 aacggtatga agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac
    14881 gatatgaagg cgctgtatga catggtggat gtaaacgtct atcaggaaaa tatcttccat
    14941 accaagatgt tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa
    15001 gatttaaccg acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag
    15061 atttattacg ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg
    15121 ccagatttat ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat
    15181 cctcaacgcc gcatagcgac aggctttctt cgtcgtagta gttcatcccc tcttccattt
    15241 catcgccagc gaattccagt tgattggcgc gaaccatcac ctcttcagcg tccatccaca
    15301 gggtgtattc atgccctgcc cgttgccagg accgttcgct acctttcagt gcgtgcgctg
    15361 cttgttccac ttcatcaagc aaggccaggt tttctttcac ctcttcatta aaccagtgcc
    15421 cgaccacttc atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa
    15481 attcgtaatc catagtgtgt tcctctgctc ccggcaatgc gccgaaatcg catcgcgcca
    15541 ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg aacggcgata gtttagaaat
    15601 aaaaaatgcc cgggaatctc attcaccggg cattgtgtcg tttatgcgca gcgcgtgcgc
    15661 ttacttatta caccgcagtc tggaaaatca ccccatcggc tttttcggtg tactgagaaa
    15721 gctggttgaa gttcagataa cgataagtat caacggcagt tttatctact tgcgcaacgt
    15781 aggtctggta ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca gccgccagtt
    15841 ctgcagaagc caggaagaca ttcgcgccag tacccaaacg gttcgggaag ttacgggtag
    15901 aggtggaaac caccgtcgca ccgtctgcta cacgcgcctg gttacccata cacagggaac
    15961 agcccgggat ctcgatacga gccccgctct taccgaagac gctgtagtag ccttcttccg
    16021 tcagttgcgc ggcgtccata cgggttggcg gtgccaccca caggcgggtc ggcaactgac
    16081 ctttgtgcgc atccagcagt ttaccagcgg cacggaagtg accgatgtta gtcatgcaag
    16141 aaccgataaa tacttcgtcg atcttctcgc cctgtaccgc agacagcgga cgtgcgtcat
    16201 ccggatcgtt cggagcacac aggattggct ctttaatctc cgccagatcg atgtcgatca
    16261 ctgccgcgta ttccgcatct gcatcggctt ccagcagctc aggattcgcc agccattttt
    16321 ccatgccctg aatacggcgt tccagggtac gacgatcgcc gtaaccttcc gcgatcatcc
    16381 acttcagcag gacgatgttg gagctcaggt attcgatgat cggttctttg ttcagcttga
    16441 tagtacaacc agcggcagaa cgctcggcgg acgcatcggt tagctcaaag gcctgctcaa
    16501 ctttcagatc cggcagacct tcaatttcca gaatgcgacc agagaagatg tttttcttgc
    16561 ctttcttctc aacggtcagc agaccttgtt tgatcgcata cagcggaatg gcgtgtacca
    16621 gatcgcgcag ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga acggattccg
    16681 gcatatccag cggcattacg ccagttgcgg cagcaaacgc caccagacca gaacccgccg
    16741 ggaaagagat accgatcggg aaacgggtat gggagtcacc accggtaccg acggtatccg
    16801 gcagcagcat acggttcagc caggagtgaa tgacgccgtc acccggacgc agcgacacac
    16861 cgccacggtt cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg
    16921 gatacgccgc ggtgtggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg
    16981 ccaggtcttt cagttcatca cgggtcatcg ggccggtggt gtcctgagag cctacagaag
    17041 tcattttcgg ttcgcagtac gcgcccggac gaatgccttt cacgccacag gcacggccta
    17101 ccattttctg cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga
    17161 acacatcact gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgcccga
    17221 taatcagcgg aatacggccg ccagcacgca cttcatcaat cagcacgtcg gttttcagtt
    17281 cgaaggtcgc cagcagttcg cctgtttcgt ggttacgcac ttcacctttg tacgggtaaa
    17341 cgtcaatcac gtcgcccatg ttcaggttag agacgttgac ttcgattggc agtgcacccg
    17401 cgtcttccat cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc
    17461 cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttcgtgg
    17521 cggatttacg cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt
    17581 tctgttgcag agcttcaatc tgtttgatcg ggccaacaac acctggctgg tctggctcaa
    17641 taccttcacg ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc gggcgtgacc
    17701 acgcatccgg tgccggagaa aggtcatcgg tgttggtttc gccagtgact ttaaagacgg
    17761 taacggtcag tttttcagcc agcgccgggc gattcaggaa ccattcggca tccgcccagg
    17821 actgcattac ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga
    17881 agttatcgaa catcagcagc gtgtgagaaa gtgctttggc agcaataggt gccagtttgg
    17941 catcatccag cgcgtcgatc agcggatgaa tgttgtaacc accctgcatg gtgcccagca
    18001 gttcgatagc tttttccgga gtcagcagag gggatttagc ttcgccttta gcgatagcag
    18061 ccaggaagcc tgcttttaca taggcggctt catcgacgcc tgggggaaca cggttggtta
    18121 acagatctaa caggaattct tcttcgcccg cgggcgggtt tttcagcagc tctacaagcg
    18181 cggccatttg gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta
    18241 cgtgcttacg gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc
    18301 aggcgattct cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc
    18361 aacgggcaga atagcaggat tttgacgacg tgttaatctg tttacaaaaa agcaacataa
    18421 aaaagtggct gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt
    18481 aattcgcatg gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact
    18541 accgcctctt tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga
    18601 tttccttgag atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct
    18661 aatttgcagt gccgggcttt gtgctgcctc tgccgggcta tatataaaaa gccgcacagt
    18721 ggaagcgcct gtagaaccgc aatcgacaca actggctgtg tctgacgctg ccgctgttac
    18781 gcttcctgca acggtttccg cacctcccgt aatacccgca gtcgtcaaat ccgcattcag
    18841 cactgcacaa atagatcaat gggtcgcacc cgtcgcgctg tatcccgacg ccctactttc
    18901 gcaggtgctg atggcatcaa cctatccgac aaacgttgct caagcagtgc aatggtcgca
    18961 cgataatcca cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga
    19021 cgccagcgtt aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc
    19081 gcaatgggtg caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg tgatggactc
    19141 ggtacagcga ttgcggcaac tggcgcaaca aaccggctcg ctgaagtcat caaccgaaca
    19201 gaaagttatt accacaacga agaaagctgt accggtaaaa cagacagtca cggcacccgt
    19261 cataccatcc aataccgttt taactgccaa ccccgtcatt acagagccgg caacaaccgt
    19321 catttccatt gagcccgcca atcctgatgt ggtctatatt cccaactaca acccaaccgt
    19381 ggtttacggg aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg
    19441 agaaccgttt gttgacagct ttgtgcgcgg attcggctat agcatgggcg ttgctaccac
    19501 gtacgcacta ttcagcagca tcgactgaga tgacgacgat catgaccatc atcatcatga
    19561 cgatgatgat tatcatcacc acgatggcgg tcatcgtgac agtaatggct ggcaacacaa
    19621 cggcgacaac atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg agcatcttac
    19681 tgataagaat atggcatggc ggcacaatcc aaactaccgt aatggtgtgc cctatcatga
    19741 tcaggatatg gcaaagcggt ttcatcaaac cgatgtcaac ggcggaatga gtgccacgca
    19801 gctacctgct ccaacacgcg acagccagcg tcaggcggca gcaagtcagt ttcagcaacg
    19861 aacacacgcc gcccccgtca ttacacgaga tacccaacgt caggcagcgg cacagcggtt
    19921 taatgaagct gaacactatg ggagctatga cgacttccgc gacttcagcc gtcgccaacc
    19981 actgacccag cagcaaaagg acgccgctcg tcagcgttat cagtcggcct ctcctgagca
    20041 gcgccaggca gtccgcgaga aaatgcagac taacccgcag atccagcagc gaagggaggc
    20101 ggcgcgccag cgtattcagt ccgcctcgcc tgagcagcgc caggcggttc gcgagaaaat
    20161 gcagactaac ccgcagaacc agcagcgaag agaggcagcg cgtgagcgta ttcagtctgc
    20221 aactcccgaa cagcgccagg tgtttaagga aaaagtacag cagcgcccac tgaaccaaca
    20281 gcaacgtgat aacgcccgcc agcgtataca atcggcatca cctgaacaac gtcaggtttt
    20341 tcgggagaaa gttcaggaga gccgcccaca acgtctaaac gacagtaacc atactgtcag
    20401 gctgaataac gagcaacggt cagcagtacg cgaacgtctc tctgaacgtg gagcaagaag
    20461 actggaaagg taaattgcag acgtaaaaaa agcggcgtgg ttagccgctt ttttaattgc
    20521 cggatgttcc ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg caggtcggta
    20581 atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt cgggtgcgcg
    20641 tggatggtca gtgcgatgtc ttcagcatca caacccattt cgattgccag gccgatttca
    20701 cccagcagct cgccgccgtt ggtaccaaca atcgcaccac cgatcacacg gtgagattct
    20761 ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcag aagcgatagc acgaccagaa
    20821 gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc ttctttctca
    20881 gtcagaccta cccatgcaac ttctggttcg gtataggcga tggacgggat aactttcgga
    20941 tcgaagtagt gtttcttacc ggcgataact tcagcggcaa cgtgaccttc gtgaacacct
    21001 ttgtgtgcca gcatcggctg accgacgata tcgccgatag caaagatgtg cggtacgttg
    21061 gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt caacttccac gccagctttg
    21121 cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac ggcgtcgtaa
    21181 cgctgcggtt cagctggtgc ttttttgcct tccatcgtca cataaatacc gtcttctttc
    21241 gcttcaacgg cggtaacttt ggtttccagc atcaggttga atttcttgct gatacgcttg
    21301 gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa catttcaacc
    21361 acgtcaatct gtgaacccag cgcgtggtaa acggtgccca tttccagacc gatgataccg
    21421 ccacccatta ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc agtggagtcc
    21481 cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga acccgctgca
    21541 atgatcgcat tgtcgaagtt gatcacggtt ttgccgttct caccttcaac ttccagggtg
    21601 ttagccccgg tgaatttacc cagaccgttg accactttga ctttgcggcc tttcgccata
    21661 ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg aatcttgtca
    21721 atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt ggcttcttcg
    21781 ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt caggcaaaca
    21841 ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc agcgcaacgg
    21901 aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt tttgatttca
    21961 gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt ctttcaggcc
    22021 gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc tcctggggat
    22081 tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag taatacccta
    22141 accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa tttttaaaca
    22201 acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg agattaccag
    22261 aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg aatgtcagac
    22321 agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat cacgcggtgg
    22381 tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc attccacacc
    22441 ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt cacaatcggc
    22501 gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc gccctgcatt
    22561 tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag ctcgcgagac
    22621 agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag accgttcggg
    22681 gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg accgtcttcc
    22741 gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc tttcatgatg
    22801 aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc gttctgctgt
    22861 ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac atgcgggatc
    22921 atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc cagttccact
    22981 tcttcgattt cgccaaactt gctgaagtcc accttcggcc acggcagcat gccagggata
    23041 ccaccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt cacgtaagcc
    23101 tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt cgccaggtta
    23161 acgccaaact cgcgtgccag acggcggatc agcggggtcg cgtgaacgta agcgtcgttt
    23221 tcagcaaatt cagatttgcc ttccgctttc gcagccggag cttcagcttt tgctgccgga
    23281 gccggcgctg ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg
    23341 aagatcataa tcagcgaacc agttttcact ttatcgccaa cgttgacttt cagttccttc
    23401 acgacgcctg caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtaatc
    23461 agtgactgtt cagcggcaac tttgtcgccc actttcacca tcacttcggt cacttcaact
    23521 tcgtcaccgc cgatatccgg aacgttaact tctttcacgc cagctgctgg tgcaggggtc
    23581 gctgccggag tcgcttcctg tttagccgcc ggagctgccg cgcctgcttc acccgcgact
    23641 tcgaagacca taatcagcga gccggtagac actttgtcac ccacgttcac tttgatctct
    23701 ttcacggtac ctgcaaacgg agccggaact tccatagaag ccttgtcgcc ttctacggtg
    23761 atcagcgact gttcagcttc aactttatcg ccaactttca ccaggatttc ggtcacttca
    23821 acttcgtcgc tgccgatatc cggaacgtta acgtcttttg ccgccgcagc cgcgggtgct
    23881 gctgccggag ctgcttcttt cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg
    23941 tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc aacagagact
    24001 ttgatctctt taacgatacc cgcctgcgga gatggaactt ccatagaggc tttgtcgcct
    24061 tctacggtga tcagcgactg ttcggcttca actttgtcgc ccactttgac caggatctcg
    24121 gtgatttcaa cttcatcagc cccgatgtcc ggtactttga tttcgatagc cattattctt
    24181 ttacctctta cgccagacgc gggttaactt tatctgcatc gatgttgaat ttggcgattg
    24241 cgtcagcaac cactttctta tcgatttcgc cacgtttagc cagttcgccc agcgccgcaa
    24301 ccacgacata agaagcatca acttcgaagt ggtgacgcag gttctcacgg ctgtcggaac
    24361 gaccgaagcc atcagtaccc agtacgcgat agtcgtcagc cggtacgtaa gtacggacct
    24421 gctcagcgaa cagtttcata tagtcggtag atgccactgc cggcgcgtcg ttcatcacct
    24481 gagcgatata cggaacgcgc ggagtttcca gcggatgcag catgttccag cgttcacaat
    24541 cctgaccatc acgcgccagc tcggtgaagg aggtcacgct ataaacgtca gaacctacgc
    24601 cgtaatcttt cgccaggatc tctgccgctt cacggacgtg acgcaggatg gaaccggagc
    24661 ccagcagctg aactttacct ttgctacctt caatggtttc gagtttgtag atacctttac
    24721 ggataccttc ctcagcacct tccggcattg ccggcatgtg gtagttttcg ttcagcgtag
    24781 tgatgtagta gtaaacgttc tcttgttttt caccgtacat acgctccaga ccgtcatgca
    24841 taatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc gggatagtca
    24901 gcgactgaat gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc agggtggtac
    24961 gaccggaagt accgccgatc aggaagccac gcgcttgctg gtctccagcc gcccagcaca
    25021 gatcgccgat acgctggaag ccgaacatcg agtaatagat gtagaatggg atcatcggca
    25081 gattgttcgt gctgtaagag gtcgccgctg ccagccagga acaccctgcg cccagctcgt
    25141 tgatcccttc ctgcagaatc tgacctttct cgtcttcttt atagtaagca acctgctcgc
    25201 ggtcctgcgg ggtatactgc tgaccgttcg ggctgtaaat accaatctga cggaacagac
    25261 cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga tctttgatcg
    25321 acttgttctt cagcatcacg ttcagagcac gaacgaaagc gatagtggta gagagctctt
    25381 tgctctgctc ttccagcagc gcgccgaagt cttgcaggct cggcagctca agcttctcgg
    25441 tgaagttcgg ctgacggctt ggcagataac cgtgcagttt ctgacgctga gcgtgcagat
    25501 aggtatgctc ttcagaacct tccgggaagg tgatgtacgg cagtttttcg atatctgcat
    25561 cagacaccgg cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg ttcattttct
    25621 taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa cctttaatgg
    25681 tatgagcaag gattactgtc gctttgcctt tggtttcctg cgctttcttg aatgcagcgt
    25741 agattttctt cggatcgtga ccaccacggt tcagtgccca gatctgctcg tcagtccagt
    25801 ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa gtgttcacga acgtacgcac
    25861 catctttcga tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc agctggatca
    25921 gtttaccgct ggtatcttta cgcagcagtt catcccaacg gctaccccac atcactttga
    25981 tcacgttcca gccagcacct tcgaagatgc cttccagttc gttgatgatc ttgccgttac
    26041 cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat aacgaaaacc aggttatcca
    26101 gtttttcacg ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc atttcaccgt
    26161 cgccgaggaa cgcgtaaacg gtctgtttag aggtatcttt caggccacgg tgttccagat
    26221 atttcaggaa tttagcctgg taaatagcac caatcggacc cagacccata gatacggtcg
    26281 ggaactgcca gaattccggc atcagtttcg ggtgcggata ggaagagagg ccattgccgt
    26341 gaacttcctg acggaagtta tccagctgct cctgagtcag acgaccttcc aggaaagcac
    26401 gggcgtaaac gcccggggag atgtggccct ggaagtaaac caggtcgccg ccatcctgct
    26461 cgttgcgtgc acggaagaag tggttaaagc acacatcata aatggttgcg gaagactgga
    26521 aggacgccat gtggccgccc agttcgaggt cttttttcga cgcacgcagc accgtcatga
    26581 tggcgttcca gcggatagct gaacgaatac ggcgttccag ttccagatta cccggatact
    26641 ccggttgttc ttcaacgggg atggtgttga tgtagttgct gatacctgtg cctgcggcta
    26701 cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc gatcagatac tgagcacgct
    26761 caacaccttc ttcacggatg accgattcga tcgcctggag ccagtcgcga gtttcgatcg
    26821 gatccacgtc atttgggaaa cgttctgaca tgggttattc cttatctatc taataacgtt
    26881 gagttttctg gaacctgtcc cattgaactc tcgccagaaa gctcaataag acaggttcta
    26941 cgtttagtta ccgcgctttt atatgcgctt gatttacaac atcttctgga taatttttac
    27001 cagaaaaatc actaattctt tcgttgctcc agacgacgca gagaacgctc acggcggctc
    27061 tcttcacggc ttctgtcgag caaaatttct tcgataaagg ccagatggcg atgcgatgct
    27121 tcgcgcgctt cttccggctt accgaccata atcgcttcaa atatgcgggt gcggtgacta
    27181 ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg gcggacattc
    27241 tgggccaaca tcggctccat acaccttagc agatgaagca gaaccacatt gtgggccgct
    27301 tcggtgacgg caatctgata ctggagtacg gcgtttgatt ccgcgtccag atcgccagac
    27361 tgctgcgcca gctctatggc gtggtggagt tcacggatgc gttccttgtc ttcatcggta
    27421 ctacgcagcg cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt ttcgagcaag
    27481 tcatactgtg attcaggatg gtcggagagc agctccacca gcggatcgct gaagctttgc
    27541 cataggctgc tctggacaaa agtgccgcca ccctggcgac gaagcaacaa gcccttcgct
    27601 tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga cgtcgaactg ttttgccagt
    27661 tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc cttcgaggat caaaaactcc
    27721 agttgctgct caatcacatc ggagagtttt ggttggcgga ttttgctgta ggccatgagt
    27781 tcctgtctta agccacttgc cgaagtcaat tggtcttacc aatttcatgt ctgtgacgct
    27841 aaagtaacaa agtattcacc ttatgtccat acaggttttg attgaaatca tgaaactgtg
    27901 cacattttaa caacttgaca tatataacgt ttcaaagttg taactatgca caaatgtaga
    27961 ctttacgtag gaaagggatt ttttaacgat tattaaacta taaaaatccg aattgaaccg
    28021 attcacttac caattttttg atttttaagt caataaaaac gaatttaaat tcactctgca
    28081 tattgagagg ggttgaggct gagctttaca aacggtttct ttttaagcaa ctcatcttca
    28141 accatgcata aagcgggtgc attcgctgcc gcataccatt attcttgatc tgacggaagt
    28201 ctttttgtaa caattcaaac ctccttgatg taaacaaatc aatacaacaa acggaattgc
    28261 aaacttacac acgcatcact gcgtagatca aaaaaacaat caccgcacga ggtttcatga
    28321 tggaaggtca acagcacggc gagcagctaa agcgcggcct taaaaaccgc catattcagc
    28381 ttatcgcgct gggtggcgcg atagggacag ggctattcct aggtagcgcc tccgtaatac
    28441 agtccgcagg gccagggatt atcctgggtt acgccattgc tggttttatc gcctttctga
    28501 tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc tttagccact
    28561 ttgcttataa atactggggc agttttgccg gtttcgcctc tggctggaac tactgggtac
    28621 tgtacgtttt agttgccatg gcagagctga ctgccgtggg taaatacatt cagttctggt
    28681 atccggaaat cccaacctgg gtttctgccg ccgtattctt tgtggtgatt aacgccatca
    28741 acctgaccaa cgttaaagtg tttggcgaga tggagttctg gtttgccatt atcaaagtta
    28801 ttgcggtagt agcgatgatc atcttcggcg gctggctgct gttcagtggc aacggcggcc
    28861 cgcaggcgag cgttagcaac ctgtgggatc aaggtggttt cctgccgcac ggcttcaccg
    28921 gactggtgat gatgatggcg attatcatgt tctcgttcgg tggtctggaa ctggtgggga
    28981 tcaccgcagc agaagctgat aacccggagc aaagtatccc gaaagcaact aaccaggtta
    29041 tctaccgcat cctgattttc tatattggtt cgttagccgt tctgctctca ctgatgccgt
    29101 ggacccgcgt taccgccgat accagtccgt ttgtgctgat cttccacgag ttaggcgata
    29161 cctttgtggc gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca
    29221 gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc acaacagggg aacgcgccaa
    29281 aagcgctggc gtctgtcgat aaacgcggcg taccggttaa caccattctg gtgtctgcgc
    29341 tggttacagc attgtgcgta ttgattaact atcttgctcc ggaatccgca tttggcctgt
    29401 taatggcact ggtggtatcc gcactggtga tcaactgggc gatgatcagt ctggcgcata
    29461 tgaagttccg tcgcgccaag caggaacaag gcgtggtaac tcgcttccct gctctgcttt
    29521 atccgctggg taactggatc tgcctgctgt ttatggcggc ggtactggtg attatgctga
    29581 tgaccccagg aatggcgatt tcggtatacc tgatcccggt atggctgatc gtgttaggta
    29641 tcggctatct gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa tctctctacg
    29701 ccctcacccg tacagggtga gggcaataat ctctacacca acgccccgta aatcgtcagt
    29761 agcgcaatca ccaccacgac cacgaacgag gttttcttcg ccattgaaac cgctgccttc
    29821 ggcgtctcca ccttatcgac atgcggttca cgcgccagag agaactgcgc cagacgcgtt
    29881 aatacctgat attgcgaagt atggaaatca cccagcgaag caaaccaggc cggtaacgct
    29941 ttctcaccat gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg cacccaatcc
    30001 agtacatgaa gcactgcatc aatgccggac tgtaaacgat gatgcggcgt ctggtatcgc
    30061 gccagccagt attgccatgc acgcaagaac gcatacccca tcagcgtaac gggtccccag
    30121 gttcccccca caatcagcca gaacagcggt gcaagataaa aacgaaagtt aatccacagc
    30181 aatgcatttt gcagctcacg caaatactca cgttcgtcgc agcctgccgg gacgccgtga
    30241 atcatggtga gttcgccagc catcgtggca cgggcatggc tatcattacg tgaagcagct
    30301 gtcagataag catgataatg aagacgaact ttacctgcgc caatacacag caaaccaatc
    30361 agcagccaca ccagcagcgt gggaacgttg aacaatactc cctgcaatgc gcgtaacagt
    30421 aaaaaagtca cacccatcgc aataatggtc atgcctaacg tgcgcccgag agaaaaatgt
    30481 ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct gccagtgctc gcccaactta
    30541 aacaggcgct cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt gtctccttgc
    30601 tgaccagcgc acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt ttccgatccg
    30661 gcgcaatatc acaatggccc gtcatgttat tagcgatatc gggatagcga tctatcagtg
    30721 cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt atacgccaga gtatcggtgc
    30781 cttcaagctc aatcccaata gaaaaatcat tgcagcgttc acgcccctga tactgagaga
    30841 ctcccgcatg ccaggcacgt ttatcgaaag gaacatactg gactatttca ccatcacggc
    30901 gaatcaaaca gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct
    30961 gcggatcaat agttccagtg aataatgcgt cgatccacgg accgccaaac tcgcctggcg
    31021 gcaggctaat attgtgcacc accagcaggg tgggtgtttc gtcatccagg cggcaatcgt
    31081 aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg
    31141 atctccttat atgtggtgct aatacccggt tcagagtagc atgtttctac cttatgattc
    31201 gttagctatc tggagtttta acatgccgcc tcgccgctac aaccctgaca cccgacgtga
    31261 cgagctgctg gaacgcatta atctcgatat ccccggcgcg gtggcccagg cgctgcggga
    31321 agatttaggc ggaacagtcg atgccaacaa tgacattacg gcaaaacttt taccggaaaa
    31381 ttctcgctct catgccacgg tgatcacccg cgagaatggc gtattttgtg gcaaacgctg
    31441 ggttgaagag gtgtttattc aactggcagg cgacgatgtc accataatct ggcatgtgga
    31501 tgacggcgat gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc catcccgcgt
    31561 gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag gagttgccag
    31621 taaggtacgc cactatgtcg aattactgga aggcaccaac acgcagttgt tggatacgcg
    31681 caaaacctta cctggcctgc gttcagctct gaaatacgcg gtactttgcg gcggcggagc
    31741 gaatcaccgt ctggggcttt ctgatgcctt cctgatcaaa gaaaaccata ttattgtctc
    31801 cggctcagtg cgccaggcgg tcgaaaaagc gtcctggctg catccggatg cgccagtaga
    31861 agtcgaagta gagaatctgg aagaacttga tgaggccctg aaagccggag ccgatatcat
    31921 catgctggat aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa
    31981 ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat ttgccgaaac
    32041 gggcgtggac tttatctccg tcggtgcgct aactaaacac gtacaagcac tcgacctttc
    32101 aatgcgtttt cgctaattgc tgacagattt atccgctcca tcgcgggcgg ataatctccc
    32161 cttccccaac tttcttcgta acgcctcgca aatttcttgt ctttcgctgc aacgaaataa
    32221 aaaacctcgg aatctgcttt cccgtttcgt tttttgcccc tcgcctgtgc tgatggcgtt
    32281 tgccaaagta gcaccaacca aatcaaggag cgaaacagat ggacaagcaa cgcggtttta
    32341 cacttatcga actgatggtg gttattggca tcattgccat tttaagctcc attggtattc
    32401 ccgcttatca aaactacctg cgcaaagccg cactcaccga catgctacaa acctttgtgc
    32461 cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg tggattagat acctgcgacg
    32521 gtggcagcaa tggcattccc tcgcctacca ccacccgcta tgtttcagcc atgagtgtgg
    32581 caaagggcgt ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc gtcgtcatga
    32641 cgccaggttg ggataacgca aacggcgtca ccggctgggc gcgcaactgc aatattcaaa
    32701 gtgacagcgc attgcagcaa gcctgcgaag atgtcttccg ctttgatgac gccaactaag
    32761 gagcggcaat gaatattcca cagctcacgg ccctgtgcct gcgttatcag ggagtcttgc
    32821 tggatgccag cgaagaagtg gttcatgttg cggtggtcga tgccccctca catgagttgc
    32881 tggacgcatt gcatttcgct accaccaaac gtattgagat cacctgctgg acgcgccaac
    32941 aaatggaagg tcacgccagt cgcacacaac agacattacc cgtagctgtt caggagaagc
    33001 atcagcccaa agcagagttg ctgactcgaa cgttacaatc tgcgctggaa caacgcgcgt
    33061 ctgatattca tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg
    33121 tattgcatcc tttaccggat gtttcaccgg atgccggagt cgcattaaca gccagattaa
    33181 aagtgctggg aaacctggat attgcggaac atcgcctgcc gcaggacggg caattcactg
    33241 tcgaactggc aggaaacacc gtctcatttc gtattgcgac cttaccatgt cggggtggtg
    33301 aaaaggtggt attaaggttg ttacagcagg tgagccaggc actggatgtt aacacgctgg
    33361 gaatgcagcc gttacaactg gcggactttg ctcatgcctt gcaacaacca cagggactgg
    33421 tgctggtaac tggccctaca ggcagtggca aaacggtcac ctggtgttgt ccacgctaca
    33481 caccaattcc accactgaaa cgctggtacg tttacagcaa atgggggtcg cccgctggat
    33541 gctatcatcg gcgcttacgc tggtaatagc ccagcgtctg gtacgtaaac tttgcccaca
    33601 ttgtcgccga cagcaagggg agcccatcca tattccagac actgtatggc cgtcgccgct
    33661 gccccactgg caggcacccg gttgtgtaca ttgctaccac ggtttttatg gtcgtaccgc
    33721 cttgtttgaa gtcctgccca ttacaccagc catacgccag cttatttccg ctaatactga
    33781 agttgaatcg ctggaaacgc acgcccgaca ggcgggtatg cgtacgcttt ttgaaaacgg
    33841 ctgcctggcc gtggagcaag gcttaaccac ctttgaagag ttaatccgcg tactggggat
    33901 gccgcatggc aagtaagcaa ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc
    33961 aagatgggat gctatgggca gagagccgta ctttactgct tatggcacta cagcaacaga
    34021 tggttacccc actaagcctg aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata
    34081 aaagcgcgga agtcattcat caactggcga cgctacttaa agccgggtta acgctttctg
    34141 aagggctggc tctgctggcg gaacagcatc ccagtaagca atggcaagcg ttgctgcaat
    34201 cgctggcgca cgatctcgaa cagggcattg ctttttccaa tgccttatta ccctggtcag
    34261 aggcatttcc gccgctctat caggcgatga tccgcacggg tgaactgacc ggtaagctgg
    34321 atgaatgctg ctttgaactg gcgcgtcagc aaaaatccca gcgtcagttg accgacaaag
    34381 tgaaatcagc gttgcgttat cccatcatca ttttagcgat ggcaatcatg gtggttgtgg
    34441 caatgctgca ttttgttctg ccggagtttg ccgctatcta taagaccttc aacaccccac
    34501 taccggcact aacgcagggg atcatgacgc tggcagactt tagtggcgaa tggggctggc
    34561 tgctggtgtt gttcggcttt ctgctggcga tagccaataa gttgctgatg cgccgcccga
    34621 cctggcttat cgcgcggcag aaattgctgt tacgcatccc gattatgggt tcactgatga
    34681 ggggacaaaa actcacgcag atctttacga ttctggcgct gacacaaagt gcaggcatta
    34741 cttttttaca gggcgtagag agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac
    34801 ttctgacaca aatccagcac gatatcagta acggtcatcc catctggctg gcgctaaaaa
    34861 atgccggtga gtttagcccg ctctgtttgc aattagtgag aacaggagag gcatccggct
    34921 cgctggacct catgttagac aacctcgccc atcatcatcg ggataacaca atggcgctgg
    34981 cggataacct cgcagcctta ctggaaccgg cgttgctgat cataacggga ggaattatcg
    35041 gtacgctggt ggtggcaatg tatctgccaa ttttccattt aggcgatgcg atgagtggga
    35101 tgggataacg ctggcgtgtt gctccacgcc agcgttggga gattacaggt tgttgaagat
    35161 gcggttttct tgttccagca cacgaataaa cgtggtgcgc ttggtcagct ctttcaggcg
    35221 tgaagcccca acgtatgtac aagctgaacg caggccgccc aaaatatcac gcgcggtatt
    35281 ttcaacctgg cctcgcagcg gcagcttaac ggttttacct tctgctgcgc ggtattccgc
    35341 aacgccgcca acgtgacgtt tcatcgcaga ctcggagctc atgccgtaga acagcataaa
    35401 tttctcgccg ttctcctcaa cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc
    35461 gccaagcatg acgaaatcgg caccgccgcc aaaggctttc gccacatcgc ccggcgtggt
    35521 acagccaccg tcgctgacga tcattccgcc cagaccgtgc gccgcatcgg cacattcgat
    35581 taccgcagaa agctgcggat aaccgacgcc tgttttgacg cgagttgtac aaacagaacc
    35641 tgggccaatg ccaactttaa cgatatcagc accggagagg ataagctcct cacacatttc
    35701 accagtcact acgttgccag cacaaatggt tttggtcggc cacgcttcac gcgctttcgc
    35761 aacgaactgc acgaagtgtt cggaataacc attcgccaca tcaatacaaa cgaagtttaa
    35821 tgccgggttc aggtcgagaa tctgtttagt tttttcgaaa tccgcatcag acgtgccggt
    35881 agaaaccatc acatgtttca gcacatcagc ggaagaattg ttgataaacg cttgccactc
    35941 ttcgacagaa aagtgtttat gcacagcagt cagaatatca aaagaagcca gcgcagaggc
    36001 catagaaaat gtgcctacgg tgtccatatt tgcggcgata atcggcacgc cggaccagct
    36061 ctgacctgaa tgtttgaagg tgaattgacg ttccagttca acatcggaac ggcttttaag
    36121 agtggagcgt ttagggcgga tgagaacgtc tttaaaacct aacttcagat cttcttcaat
    36181 acgcatgtgc ggattcctgg ggttaatggc tataagggta agagcgcaac tccagtgacg
    36241 ctatcatacg cactaatcaa tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca
    36301 atcccataaa tttacctggt aaatagtgag cgaacgtttc atcaatttca ctttcgcgcg
    36361 ctcattttta acgttttgat aatcatgatg aattcccggg aatataaatt atggggtata
    36421 tagttgcctt aacgggaggc attggcagtg gtaagagtac cgttgccaat gcgtttgctg
    36481 atctcggaat taacgtcatt gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg
    36541 cacctgcgct acatgccatt gctgatcact ttggcgaaaa catgattgct gctgatggaa
    36601 cattgcagcg ccgggccttg cgcgagcgga tcttcgccaa cccggaagag aaaaactggc
    36661 ttaacgacct gctgcatccg ctgattcaac aagagacgca acaccagatc cagcaagcaa
    36721 cttcccccta tgtactgtgg gttgtgccat tgctggtaga aaactcactg tataaaaaag
    36781 cgaatcgagt gctggtggtg gatgtcagcc cagaaacgca acttaagcgc accatgcagc
    36841 gcgatgatgt aactcgcgaa catgtcgaac aaatccttgc tgctcaggca acgcgcgaag
    36901 cccgccttgc cgtggcagat gacgtcattg ataataacgg cgcaccggat gctatcgcat
    36961 cggatgttgc ccgcctgcac gcacactatt tgcagcttgc gtcgcagttt gtctcacagg
    37021 aaaaaccgta atgcagaccc aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac
    37081 atggctgcgc attgagtttt tgattcagca actcaccgtt aatttaccca tcgttgacca
    37141 cgctggcgcg ctgcatttct tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg
    37201 cgaagtccgc actgagctgt tgaaagaact taaccggcag caacgtaaac tccagacctg
    37261 gattggcgtg cctggcgtgg accagagccg tattgaagca ttaattcagc agttaaaagc
    37321 ggcggtgagc gtattaattt ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt
    37381 gattgctctg gtgcgtcagc gactgagcat cccaggcggc tgttgcagct ttgatttacc
    37441 tacattgcac atttggctgc atctacccca ggcgcagcgc gacagccagg tagaaacctg
    37501 gattgccagc ctgaacccgc tcacccaggc acttaccatg gtgctggatt taattcgcca
    37561 gtcggccccc ttccgtaaac aaaccagcct gaatggtttt tatcaggata acggtggcga
    37621 tgccgacttg ctgcgcctga atctgtcgct cgattcacag ctttatccgc aaatttccgg
    37681 acataagagc cgttttgcca ttcgttttat gccgctggac actgaaaacg gacaggtacc
    37741 ggaacgtctg gatttcgaac tggcctgttg ctaaggagta aaaatgtcag aaactattac
    37801 ggtgaattgc ccaacctgcg ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc
    37861 attttgctcc aaacgttgtc agctgatcga cctcggagaa tgggctgctg aagaaaaacg
    37921 aatccccagc agtggcgatc tttccgaaag cgatgactgg agcgaagagc caaagcagtg
    37981 acatttgtct gatgcctcac gtaggcctga taagacgcgg acagcgtcgc atcaggcatc
    38041 ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac ctacagacgc ttaagcttcg
    38101 caattaccgg ttcattggct ggcggaaaat catcggcatt aagaccgacc agcgacatcc
    38161 actcaccggg ttgcccttct ttaccccacg gccccccttc ccaactttcg accagccaga
    38221 accacagagt tatatgcctg tccgggaatt catattccag tttttcaaat agcgaaaaat
    38281 gttgtggggt aatcccgacc tcttcctgaa gttcacgcac caccgcctgt tccggcgttt
    38341 cgcccatttc aattttaccg ccgggaaact ccagtttatt cgccatgtgc gcatctgctg
    38401 cgcgacgtgt tataaagatt tcattgttct cgttgcgaat aatacctacc gcaatttgca
    38461 gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac
    38521 tgttagcttt tattgcaggc gaccatggca ctgcttgtat tttttaccag aaccgcacgg
    38581 gcaaggatcg ttacgtccta ctttgcgttc accggtttgc gccgccagtg cagctgcggc
    38641 tgcagagtcg tcatcctgat ggctaagctg ctgcatttgt gctaaacgct cggcttccat
    38701 acgacgctgt tgttccagct cctcaacctc ttcaggcata cgtacctgaa ctttgctcag
    38761 cgtactgata acttcatatt tcaacgactc cagcatcgct gcaaacatgg agaacgattc
    38821 acgtttgtat tcctgcttcg gatctttctg tgcatagcca cgcaggtgga taccctgacg
    38881 cagatagtcc atcgctgcca ggtgctcttt ccacagggag tcaagcgttt gcagcatgac
    38941 gcctttctcg aagtgacgca tcatctcagc accaaccact tcttctttac gctgatacac
    39001 ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct tcatgcagtt ctggttcttt
    39061 atccagccac tcggtaattg gtaaatcgag gtcgaaatcg ttcttcagac gttcctgcag
    39121 ccccggaata tcccacattt cttccagcga ctgtggcgga atgtaggcat caatggtggc
    39181 tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc acatcgctga catccagcag
    39241 ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta gccacgtcat catattccag
    39301 cagttgctta cgaatgtcga agttacgact ttcaacttta cgctgggcgt tggcaatcgc
    39361 tttagtcacc cacgggtgtt caatggcttc gcctggcttc atacccagtt tacgcatcat
    39421 gccggatact cggtcggaag caaaaatacg catcagcgca tcttccatcg acaggtagaa
    39481 acgggaagaa ccagcatccc cctgacgacc agaacgaccg cgcaactggt tatcgatacg
    39541 acgggattcg tgacgctcgg taccgatgat atgcaggcca cctgcttcca gtaccgcatc
    39601 gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc tctgcggtcg gattttccag
    39661 cgcggcaact tctgcctgcc agctacctcc gagcacaata tctgtaccac gacccgccat
    39721 gttggtcgcg atagtcaccg cagccggata acctgcctga gcaacaatcg ccgcttcgtt
    39781 ggcgtggaat ttggcgttca ggacattgtg cttaataccg gctttggtca gttcgtttga
    39841 caccagctcc gatttttcga tggagatagt acccaccagc accggctggc ctttcgcagt
    39901 acgttctttg atatcttcaa tgatcgcctg aattttttcc gcttcagtca tgtagaccag
    39961 gtccggcaga tctttacgaa tcattggacg gttggtcgga acaacgacgg tatccagttt
    40021 gtagatggag ctaaattcga aagcttcggt atcagcagta ccggtcatcc ccgccagttt
    40081 ttcatacaga cggaagtagt tctggaaggt gatcgaagcc agcgtttggt tttcgttctg
    40141 gatctgcaca ccttctttcg cttccacagc ctggtgcaga ccatcggacc agcgtcggcc
    40201 ctgcatggta cgaccggtgt gttcgtcaac gatgataact tcaccatctt taacgatgta
    40261 gtcgacgtca cgggtaaaca gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag
    40321 catgatgttg gccggagagt acagagactc cccttcatcc atgatgccct ctttcaccag
    40381 cagttcttca atcagcacca gaccacgttc ggtcaggttc acctggcgag atttttcgtc
    40441 caccgagaag tggccttcgc cctggaaggt ttcggagtct tctttttcct gacggatcag
    40501 gtgcggaata attttattca cgcgtttata catttccgag ctgtcttctg ccgggccgga
    40561 aatgatcagc ggtgtacgcg cttcatcgat caggatggag tccacttcgt ccaccagcgc
    40621 atagtgcagt ttacgctgta cacgttcttc agggctgaac gccatgttgt cgcgcaggta
    40681 gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt
    40741 tgccggtgct ggcatgcccg gcaggttgat accaacagtc aggccaagga attcaaacag
    40801 cggacggttg ttttcggcgt cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac
    40861 gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc
    40921 accggtacgc atttcggcga tgcagcgttc gttaagaacc ataccgccga gtaactgaac
    40981 gtcgaagtga cgcataccaa agacgcgctt acttgcctca cgcaccacgg cgaaagcttc
    41041 cgggatcaga ttttccagca cttcgccttt ttccagacgc gcacgaaact ctgcggtttt
    41101 ccctttcagt tcttcatcgg agagtttttc catctccggt tccatggcat tgatgatgtt
    41161 gaccactttg cgcatccggc gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa
    41221 caatttgatt agcataataa aatctcaaac gccccgcgtt acggagttaa taaaatgaag
    41281 taaaggttta ttgttgttag gtgaggcgtt gagggccagc acggatgcct tgcgcctggc
    41341 ttatccagac gggcgtgctg aattttgctt gtggggtaaa atgcgcataa tcaatgcgat
    41401 aacccttttc agacggcgtg ccttcctggg tcagcagcgc gctgagcgta tccagtaatg
    41461 caagatgttg cgcctgaaga ggcaaagatt cttcagcaac gggcagtgtt tgcggtgcca
    41521 ttgcgaaaga aagatgacgg attaccgtac gaatagcatg ttgatgccag taatcaacgg
    41581 aatagttcga attcgggcgg cgcgtgttcg cttccagcaa ggccaattga ccaaagttaa
    41641 ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg
    41701 cgttgctgag cgcaggcaaa cctaaactcg ccgcgaccat ccctaataag agatgcggcc
    41761 agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat tccactcacg ttattgccat
    41821 cccgatcgaa ttcaaacgta tttagcgcac aaatcttatc attaaagcct gaagtccaca
    41881 gcacgaatga agataagttg tgcgtaaaag tgcttaagaa aacagcgttc gcaccgtttt
    41941 ttatagctct tataaaattc aaaaaaaaac gactggttca cctggccgga gagagtgcca
    42001 gatttgccag tcgaatttta tacgacagta taaatgtcgt tatgccagta cagctgaagg
    42061 cgctttgaag gccaacggca gttctgcgtc gtcctggaag gtcacatatt cccaggcttc
    42121 ctgtttcgcc aggacagcct gcagcagttt gttattcagt gcatgaccgg atttataagc
    42181 ggtaaatgca ccaataatat tgtgaccaca catgaacaag tcaccgatcg catcgagcat
    42241 tttgtgacgc acaaattcgt cttcaaaacg caggccgtct tcgttcagta cgcgataatc
    42301 gtcaacaacg atggcacaat cgaagctgcc gcccaggcac aaaccacggg actgcagata
    42361 ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg atctggcgca taaacgcatc
    42421 agcggagaag ttcatcgcat agcgctggtt gctggaatca atagccggat ggttaaaatc
    42481 gatggtgaaa tccagcgaaa aaccattgta cggcttaaat tcagcccact tatcgccatc
    42541 ttcgacacga acagtctctt tgatgcgaac aaattttttg gcgcagttca actcgtcgat
    42601 accggcgtca agcagcaggt atacaaacgg agcggcgctg ccgtccatga tcgggatttc
    42661 cggcgcgtta acttcgataa caatgttatc gatgcccagg cccgcgagag cagcattgag
    42721 gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc agacacgtac agagcatggt
    42781 atcacgcaca gatttggcat cggccgggaa atctaccggt ggattcaagt cggtgcgacg
    42841 atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc agggtgactt tcttgccggt
    42901 atgtaaaccg acacccgtcg cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt
    42961 attatctcgc caaattacct atccaaccga agtgtactat acattcggcg ggccagttta
    43021 gcacaaagag cctcgaaacc caaattccag tcaattctta atcagcttgc ttacgtagga
    43081 atgctgggat atccagataa tccggctctt tcgcagtttg cggcgcattg tcattcacta
    43141 ctttagcaac cggcttctgc tcctgggtca gcggagccat cccatgctgc tggtagcgat
    43201 ccatcactgg ctgctgaacc tgcttattgg tcaccagagt gatttcagga cgtttgtcca
    43261 tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc attcatatcc gggtcaagag
    43321 aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg
    43381 tttcgaactc atccagacgc aggtcgaagc ccgccgtgat gttaaccagc acgccgcgcg
    43441 cgccagacag gtcgatatct tccagcagcg gagaagagat agccatttca gcagcttctt
    43501 ccgcacggtc ttcaccgctc gccacgccag aacccatcat tgcgtagccc atttcagaca
    43561 ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc cggacgagta atcagttcgg
    43621 cgataccttg cacagcgcct ttcagtacat cgttcgctgc gccaaacgca tccagcaggg
    43681 agataccgcg gcccagaact ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca
    43741 catgcttgga cagttcagtg atcccctgct cggcgaatgc catacgcttc ttgccttcaa
    43801 agttgaaagg cttagtgacg acagcaacgg tcaggatacc caaatctttt gccacttcag
    43861 cgacgactgg tgctgcacct gtaccggtac caccacccat acccgcagca ataaagacca
    43921 tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc ctcatcagcc gcattgcggc
    43981 caacttctgg attagcgcca gcgcccagtc ctttggtgat accgctaccg atttgaatcg
    44041 tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa
    44101 caccttcaat acgctcgcgc accatgtgtt caacagcatt accgccgccg ccgccgacgc
    44161 cgatgacttt aatcaccgcg tcattggtaa gttccattgg ttcaaacata gtttctctcc
    44221 gatttgtgcc tgtcgcctga gtccgtaatc atcgtcggcc tcataaaaat taaaactctt
    44281 ttcgcagcca actattgagt cgcttgatcc acgagccaac tgatgctgta acacgttttt
    44341 ctacttcagc ttcaccgtta agatgtgact ctttcccata gtgaagcaat cccaccgccg
    44401 tcgaataata cggctcctga gcataatccg ttaaaccggt aatgttcagc ggcgcgccga
    44461 tacgcacttg cgtatgaaac acgcgctgag cacaggctgc aagaccttcg atctgcgctg
    44521 cgccaccggt taatacaatg cctgccgcca ggtgatgttt aaccccttgt tggcgaagct
    44581 tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag caactcggta tagcgcggct
    44641 cgatcacctc tgccagggtc tgacgttgca gactccgcgg cggacgaccg cctacgctcg
    44701 gcacttccac gctctcatct tttccaacga tggaacccag cgcacaaccg tggcgaactt
    44761 taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta agcgatatca ctggtcacga
    44821 cattgccagc ataaggaatt acctttgtgt ggcgcaatgc cccaccggta taaacggcga
    44881 tatccattgt accaccaccg atatcgacga cgcagacacc cagttcacgt tcatcttccg
    44941 tcaataccga ataacttgat gccagtccgg caaatatcag ttggtcaact ttcagcccac
    45001 aacgttcaac cgctttgacg atgtttttcg ccatatcgtt gtgacatgtg atcaggtgca
    45061 cttttgcctg catccgcaca cccgaaagtc ctaccggatt cttgatccct tcctgatagt
    45121 caatcgcata ctcttgcggg atcacatgca gcacacgatg ctcatcgcgc acacgcaccg
    45181 atttcgcggt atggacgacg ttttccacat cttcttgcgt cacttcttct tcagaaatag
    45241 gcaccatacc aatttcattc tggcagctga tgtgcttacc agaaagcgcc agatataccg
    45301 aagagatctg acaatctgcc atcaattctg cctggtcaat ggcgcgttgt acgcacttga
    45361 ccacggattc gaggtcgttc accccgcctt tatccatacc acgtgacggg cagctgccca
    45421 cgccaatgat attgaccata ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg
    45481 cggtaccaat ttccagtcct actaccagtt ttctgtccgt cgccttgatc attgttgttc
    45541 tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc ggcaagggcg cccagcctac
    45601 tgccgctcca gagtcataac gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc
    45661 ctgctgctgt aaaaccggat aaagttctac aaagcgaacc aaacgtttca tcgtatcgcc
    45721 ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt
    45781 catcgccgct tccttcagag taaatctgtc ctttgccagc atctgcccca tttcgcgata
    45841 gccctgcaac acttcattag cgctgccttc cgggccatac agcattggaa gcacctgctt
    45901 gctggtgcga tctggcggca cgctgaaggt gtttccttcc gcgtctacca tgtgttgatc
    45961 attccaccgc gcaatcggca catattcaac cagatgaatc ttcaattcat caggccactg
    46021 ctttctgacg ctcacctgct taatccacgg caggcgttgt tctatttgcg tctggatgat
    46081 gttgacatcc tgggtcataa aggtacccgg ctcacccaat gccaggatcg actgccggat
    46141 atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg
    46201 ttgcgcatct tccatccagc tcaacacgac ccagccgctc accaacactg tcgttaaaac
    46261 ggtcagcagg aaaaggatcc ccgccagacg cgttccatta ttgcggcgag aagaaacctc
    46321 ttcttcgctg tttcgcgtgt tcagagcagc ctgcgacata ttagtccgcc agttccagaa
    46381 ttcgtactac caactgcgag aagctcatac ctgcctgacg tgccgccatc ggcaccaggc
    46441 tgtggctggt catacccggt gaggtattgg cttccagcag ataaaactgt ccatcactgt
    46501 ccagcataac gtcaatacgt ccccatcctt tgcaacctaa cgtcgtccat gctttcagca
    46561 ctaatgcctg caaattggcc tcttgtgacg cttccagacc tgcggggcag aaatactgtg
    46621 tctcatcaga gagatacttc gcctcataat catagaaggt tccggacggt tgaatacgta
    46681 ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa ctccggccca cttagccatt
    46741 tttcaatcaa tacttcttca tcgtgctgaa atgccaatct taatgcatct tgtagagcat
    46801 tttctgctac tacttttgac attcccacac tggaaccttc gcggctcggc ttaacgataa
    46861 ccggcaaacc cagagcagaa atttctgcta actgcttatc gctcaggcct ttttcaaact
    46921 ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc ggcaccttgc cacagaagtt
    46981 tgctgcgtag tttatctatt gaaagcgcag atgccatcac tccgctgccg gtataaggca
    47041 agcccatcag ctcgagcatg ccctgcagcg taccatcttc accgccgcga ccgtgtagcg
    47101 cgataaacac tttctgaaaa cccatcgact tcagttgcgt cacgtcgact tctttcgggt
    47161 cgacaggata cgcatcaata ccgccttcac gcagcccggc taacaccgct gcgccagaat
    47221 tcagagaaac ttcccgctca gcggaagtcc cgcccaacag gaccgcgatt ttatcagtca
    47281 tgttgttctt cctccggagt ttgcggcttc agtttgattt cagctaaaga acgggcaatt
    47341 tttccgatat taccagcccc ctgaacgaga atcaggtcgt taccggttaa taccggtgcc
    47401 agcatctcgg ctacctgcgc agggtccggc accagaatgg gatcaatttt cccacgtcca
    47461 cgaattgtgc gacacagcga acggctgtcc gctcccggaa ttggcgcttc gccagccgga
    47521 tacacttcca gcatcaacag ggtatcaacc tgcgtcagca cattggcgaa atcatcatac
    47581 aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca ttaccaggtt tttatccggc
    47641 cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg tcgggtggtg gccgtagtca
    47701 tcgaccagca ttgccgtacc gcttttacca ttcactggct ccagcgggaa ttcaccgagg
    47761 aaatcaaaac ggcgaccagt cccctggaag ctctccagcg cacgcaaaat agcctcgtcg
    47821 tcaattccct cttccgtagc aaccgcaacc gcagctgcgg cgttcagcgc gttatgacgg
    47881 cctggcgcat tcagggtgac gcacatcggc tctttgtcct gacgcagcag cgtaaagtgc
    47941 ccctgcgggc caatctgctg ataatcttct acacgcacgt cggcatcttc gctgaagccg
    48001 taagttgtgg tctgacgtcc cacacgcggt aacaattcgc ggatcaccgg atcatcaaca
    48061 cacatcaccg cacgaccgta aaaaggcagg ttgtgcagaa aattaataaa ggtctgtttt
    48121 aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc
    48181 gccaccatcg gttgcagatg caggaacgat gcatcactct catctgcttc ggcaatcagg
    48241 taccgaccat gccccaaacg cgcatgaacc cccgccgctt ttaccagccc gccgttaacg
    48301 aaggttgggt cgagccccgc ttctgcgtag atgctggaaa ccatcgcggt agttgtcgtt
    48361 ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac gcattaactc agccagcatt
    48421 tcggcacgac ggatcaccgg aatacgcgct tcatgagctg cgacaatttc cgggttgtcg
    48481 gcagaaatgg cgctggaaac aacgaccacg ctggcatcac gtacgttttc cgggcgatgg
    48541 ttgaaataaa tcgtcgcacc cagattcagt aactgctgcg tgaccggatt tggcgctaaa
    48601 tcggaaccac tgatctgata accttcattg gccagaactt cggcaatacc gcccataccg
    48661 gcaccaccaa tgccgacaaa atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa
    48721 cgcagttttg ccaattgttg tgtattcatt ctttacgcca ttaacttctt aaattcatac
    48781 gatgcaaaag gcatcgctac aattacgccc gggcagcccg gctcacttca tttgccactc
    48841 gctcggtggc atccggaatg gatgcagcgc gggcgcgttc tgccatggtt aataaggttt
    48901 ctcgcgacca cccggccagg gtgttggcga cagcatccac gctaagctgt ggctgctcga
    48961 taattttggc tgcgcccgct ttttccagcg gtagcgcatt ccagtattgc tggcggtctt
    49021 tatgttgaaa cggcacaaac aacgccggta gtcctgccgc ggcgatttca ctcacagtta
    49081 aagcaccgga gcggcaaacg acgacatccg cccacgcata cgccgccgcc atatcatcaa
    49141 taaattcagt cactttatgc tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt
    49201 gttgcgaacc tttgccgctc tgatgccaga tagtgactga atcacccagt ttcgcagcaa
    49261 cctgcggcat tgtctggtta agaatgcgtg cgccctggga accacccact accaacacac
    49321 gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa cggcagcgcc agcacatcgg
    49381 tacgcaccgg gttacccacc acttccgcat tagggaaagc gcctggaaac gcctgcatca
    49441 ctttggtagc aatcttcgcc agccatttat tggttaagcc cgcaataccg ttttgttcat
    49501 gaagtacaac cggaatgcct aacgaccacg cggccagacc acctggacct gatacatagc
    49561 cgcccatacc gagtaccacg tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc
    49621 aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt tatacctttt ccacgcaaac
    49681 cagagatacg aatgaaatca atttcgatgc catgttttgg cactaagtcc gcttccatac
    49741 ggtcggcagt ccccagccag cgaacttgcc aaccctgagc cattagatag tgcgcaaccg
    49801 ccagtcccgg gaatacatgt ccaccggttc cgcctgccat caccattaat cgctttcctt
    49861 gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc tccagacgcg tttcataatc
    49921 aatacgcaac agcatcatga tggctgtcga cataatcagt aagctcgaac caccgtaact
    49981 gattagcggc aatgtcagac ctttggtcgg taacatcccc gccgccgcgc ctacgttaac
    50041 cagcgcctgg aagctaaacc agatgccaat agaacaggcg agaaaaccgg aaaaacggtg
    50101 gtcaatttct aatgctttgc ggccaatcga catcgcacga aaagcgacga agaataccat
    50161 taaaagtgct agtaccacac cgacataacc taattcttcg ccgataatgg cgaaaataaa
    50221 gtcagtgtgc gcttccggca gatactccag tttttgtacc gaattaccta aaccttgccc
    50281 ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt aactgatagc cgctgccaaa
    50341 gggatcttcc cacgggttcc agaatgcggt aacacggcgg atacggtacg gttcggcgag
    50401 gatcagcaac acaaccgctg aaatgcccat accgataatg gcaatgaact gccacaattt
    50461 cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac aacaccacca ccgtaccaag
    50521 gtctggctgt gccagcagta acactgccaa caccagaatc acgcccatcg gtttcaggaa
    50581 gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc agatagttgg cgatatagca
    50641 aaacagcgac agttttgtca gctccgcagg ctggatacgc agcaaaccga gatcgatcca
    50701 acgcgatgcc cctttaacag cgctacccac taccagcacg atcatcagca ggatgataga
    50761 tccgagcagc atcgtggcac tgtagcgttg ccagaactcc atcggcagac gcagcgtaat
    50821 gatcgccaga ataaacgcca aaatcagata gacaccatca cgcttcgcga agaagaacgg
    50881 atcgttggtt aagcgttgcc ctatgggcat tgacgccgag gtcaccatga taaagccaat
    50941 cgccgcgagg ccgaaggtca gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt
    51001 atctttttcc cgcgagccca tcacccagcc ctttagcgcc gtggagatcc agaccaggat
    51061 actgaatcct ggcaggcgcg gcattttcag gcgagggaga gataaacgca tcaacctaac
    51121 tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa agttcttgaa ctgatcaagg
    51181 ctggcacagg ccggggagag cagaaccata tcgcccggct gaacacgcgg agccagcaag
    51241 cgcatagcct gttccatagt ttcggtttgt tctgccactt ccgggcgtag cgccgccagc
    51301 tgcgcgccgt cacgaccgaa acaatacaga cgtacgttat cgccattcag gtaacgcacc
    51361 agtgggctaa agtccgccga tttaccatcg ccgcccagca acaaatgcag cgtgccgtct
    51421 acgtgcaggc cattcagcgc cgcttccgta ctgccgacgt tggtcgcttt tgaatcgtta
    51481 atccagcgca caccgttatg ctccagcacc acttcaaagc gatgcggcag accggtgaag
    51541 gtggttaacg ctttcaggct gctggcgcgc ggtaaccctg cggcatctgc cagcgccagc
    51601 gccaccagcg cattggtgta gttatgctgc ccggaaagtt tcatctcttt cacattcagc
    51661 actttctcgc ctttaacccg cagccaggtt tcgccctgct gatgattcag gtgatagtca
    51721 cccatgttga cgccaaagct gacgcagcgt tcatccgcac cgcgaatcgg cattgttaag
    51781 gcatcatcag cattaaccac gcaaactttc gcgttttcgt aaatgcgcag ttttgctgca
    51841 cgatactgtt gtaaaccaaa cgggtagcga tccatatgat cttcagtcac gttcagaatg
    51901 gtcgctgcta ccgcctgtaa gctggaggtg gtttccagct ggaagctcga cagttccagc
    51961 acgtacagtt cacactcatc atccagtagc atcaacgcag gcaggccaat attgccaccc
    52021 acaccaacgt taacccccgc cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt
    52081 ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg cttcgcgaca aaacagctcg
    52141 atatcgccaa cgatttcgat tccggcatca gcggcagcgc ttaaggatgg atgcgccagt
    52201 gcaataccgg gactggcgac aatcagatct gccgccatca gccattcatc attcagactg
    52261 cccgtgtggc gttctacggc ttcgggtaat ttatccaggc caggcggtgt catacgcgta
    52321 tccataacgc gcggcgtcac accgcgagcg aggaaaaagt ccacgcagga aagcccggtg
    52381 aggcccaggc cgataatgac aacattttta ccctggtaat cagccatgat taacgtacct
    52441 tcagcgttgc cagaccaatc aggaccagca tcagcgaaat aatccagaaa cgcacaatga
    52501 cgcgcggttc cggccagcct ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa
    52561 tacgttgtcc gcgcagttta aaggagccga cctgcaggat gacagaaagc gtttctacca
    52621 cgaacacgcc ccccataatc accagcagga attcctgacg tagcagtacg gcgataatgc
    52681 ctaacgcacc acctaacgcc agcgaaccga catcgcccat aaagacctgc gccggatagg
    52741 tgttaaacca caggaagccc agtcctgccc cgactatcgc ggtacagaca ataaccagtt
    52801 ccccggcgtg tcgcagatac ggtatgtgta aatagctggc aaagttcatg ttgccggtcg
    52861 cccacgccac cagcgcaaaa ccaccggcga caaatacggt cggcataatt gccagaccgt
    52921 caagaccatc ggtcaggttt accgcgttgc cagtacccac aatgacgaag taagccagca
    52981 gaatgtagaa cagccccagt tgcggcatca catctttaaa gaatgggacc accagctgcg
    53041 ttgcgggcgt gtctttgccg gcaaggtaca aagcgaaagc aacacccagc gcaatgaccg
    53101 acatccagaa atatttccaa cgagcgatca accctttggt gtctttacgc accactttgc
    53161 gataatcatc aacaaagcca ataacaccgt aacctaccag caccaccaac acgcaccaga
    53221 cgtacggatt ggacgggtaa gcccacagca gcacggagat cacaatcgct gtcaggatca
    53281 taatcccgcc catggtcggc gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac
    53341 gcactacctg accaaaggaa agtttttgca aatgagcaat catgcgcggg cccatccaca
    53401 atgagatgaa cagcgcggtc agcaggctga cgatggcgcg aaacgtcaga taggaaaaga
    53461 cgttaaagcc ggaataatat ttgaccaaat gttcggccag ccaaactaac atgtcccatt
    53521 ctcctgtaaa gcgcgtacta cctcttccat ggcagcacta cgtgaaccct taactaaaat
    53581 cgtaattacc tgttgctcag caatcagtga tttaagacgc gtaattaacg cagttttatc
    53641 agcaaaatgt tcgccaacgc cgctggcggt gctgatagca tggctttgtt tacccatgct
    53701 taacacgcgg tcaataccag cagcttttgc cgcctcaccc acctgtacat ggcaggcttc
    53761 gctttcagcg cccagttccg ccatatcgcc caccaccagc acgcggtagc ccggcatttc
    53821 agccagtacc tgaacagctg cggtcattga accgacattg gcgttgtagg agtcgtcgag
    53881 cagcaactgg ttttctgcca gttggatggg gaacagacgg ccaggaacag ctttcagatt
    53941 tgccagcccc gctttgatag catcaagcgt tgcgcccacg gacatggaga gcgcagcggc
    54001 tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc agcagaacat cgacgctacc
    54061 ggttggggtt tgtagggtaa attccatacc gtgcgaggtc acatggatat tggtggcggt
    54121 gaaatcgctg ttggcggcat tgggtgagaa acgccacact ttgcgtgagc caattacgct
    54181 ctgccagttc agccagtcgt tgttgtcggc gttcataatg gcgataccgt tttccggcag
    54241 gccgctaaag atttcacctt tcgctttcgc gacacccgca agcgagccaa aaccttccag
    54301 atgcgccgct gccaggttgt tgaccagcgc cgcttccggg cgagtcagac tcacagtcca
    54361 ggcaatctcg ccctgatggt tcgcgccaag ttcaataact gcgtaatcgt attccggcgt
    54421 taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg agattgcctg ccgtataaag
    54481 cgtgttgccg cactggctta aaatcgccgc cgtcatctct ttaacggagg ttttgccgga
    54541 ggaccccgtc agagcaacca cgagcgccgg aacttgctgg cgaacccatg cagccagttc
    54601 accaaacgcc agacgcgtat ccttgacgat taactgcggc aggtcgatgt ccagcggacg
    54661 gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg tcggcaaaat cgtgggcatc
    54721 aaaacgttca cctttcaggg caacaaacag gcagcccggc gtcagttttc gggtatcagt
    54781 ggttacagca tcaagggtga tatctgcacc ttgcagttca ccgttgagaa tatcggtaag
    54841 ttggctaagg gttacgctaa tcatgcaatc ccccccagca gacgcgccac cgtgacgcga
    54901 tcggagtagt ccagacgctg attgccaaca atctggtaat cctcatggcc tttgcccgcg
    54961 accagtacca catcattctc tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg
    55021 ccttccatca ctttggcatg tccggcatct aacattcccg ccagaatatc gttgatgatg
    55081 gcacgcggtt cttcggtacg cgggttatcg tccgtcacca ccgccacgtc agcaaactct
    55141 tcggcaattg cgcccatcag tggacgctta cctttatcgc gatcgccacc acagccaaag
    55201 acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct gtaaggcttt ttccagtgca
    55261 tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc ctggcgcagt gaacacttcc
    55321 atacgtccgc aaaccggttg cagacgcgcg gcggttttca gcagatcagc cagtggatag
    55381 cccagcgcca gcagtgttgc cagcgcgagc aacaggttgc tgacgttaaa agcgcccatc
    55441 agatggcttt caatttcgcc atcgccccag cttgaactaa agcgaatggt cgcgccgctg
    55501 tcgtgatagt tcacttcggt cgctttcaac cagcgtccgt gacagttcgg attgatatga
    55561 tcttccattg ataccgcaac cgcgtccggc agttttgcca gccagcggcg gcccacttca
    55621 tcgtcggcgt taataatcgc ctgaccgcaa tgatgttcag aataaagcag ccatttcgca
    55681 gcttcgtagt gttccatatc accatgataa tcaaggtgat cgcggcttaa gttggtaaag
    55741 accgacgccg gaaatttcaa tgccgccaca cggtgctgta ccagcccgtg ggaggaaact
    55801 tccattgcgc aaaaagtcgc gccctgatcc accagccccg ccagctcatg ctgaacatca
    55861 actgccgaac cggtagtatt ttctgtcggg atcactttcc ccagcagacc gttaccaacg
    55921 gtgcccatta ccgcgctggt ttcgccaagc agttggctcc actgcgccag aagctgggtc
    55981 gtcgtggttt tgccgttggt gcccgttacg cctacgagac gtaaattgtc agagggttca
    56041 tggtaaaagc ggcccgccag tgcagataaa cgctcgttga gctggctgag atagatgacc
    56101 ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct catctttcgc ctctgcaata
    56161 atggcagcca caccttgcgc tatcgcctgc gggatatatc gacgcccgtc cgcctgatga
    56221 cctactacag ctacaaagag atcgcccgcc gcagccacac ggctgtcgag tgtcatctct
    56281 cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa gaaggtcgcg caaattacga
    56341 tctgccacct gtcccctcgc cttgattaat cacaaattca tttttatcgc ccgttgtcag
    56401 cgcatccggc tcgatgttca tggtacgcaa tacgccgccc atgatggcac caaagaccgg
    56461 cgcggaaacg gcgccgccgt agtatttacc cgcctgcgga tcgttgataa caacaaccag
    56521 cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta taagcaatat atttattgat
    56581 gtagcgaccg tccggcccga cctttttcgc ggtaccggtt ttaatggcga tacgatagcc
    56641 tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc acgctttcca tcatatgcac
    56701 cacggtgcga acaatggatt ccgggaagac acgttcaccg ggaaccgggg ggtcaacttt
    56761 ggtaatcgac agtgggcgat aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa
    56821 ctgtaacggt gttaccatta gcccgtagcc gaaagagaag gtggccctct ctatgtcaga
    56881 ccaccgttgt ttttgaggat ataagccact gcgttctccg accaacccca aattggtcgc
    56941 ttttcccagt ccaaaacgtg agtaagtatc tactaacgct gaggacggca tcgttaacgc
    57001 cagcttggaa acaccgacgt tactcgactt ctgtaatacc ccggtcaggg ttaattcgct
    57061 gtagcgtgcc acgtctttga tttcgtggcc gttaattcga taaggaatgg tattgagtac
    57121 cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatc accaccatcg gtttaaccgt
    57181 tgagcccggt tcaaacacgt cggtgatggt acggttacgc atcgcctctt tcggcgtgcc
    57241 gctcagattg ttagggttgt atgacgggct gttagccatc gccagcactt cacctgtgtt
    57301 gacatccacc agcacggcgc taccagattc agccttgtta aaggccaccg cgttgttcag
    57361 ttcgcgataa accagcgcct gcaggcgttc atcaatactc agcgccaggt tgtgcgctgc
    57421 ctggctgtca gtagaagaaa tatcttcaat tacgcgacca tagcggtctt tacgcacaat
    57481 gcgctcaccc ggctgcccgg taagccattt atcgaaactc ttctcaacgc cctcaatccc
    57541 ttgactatcg acgttagtaa agccgatgag gtgagcagtc acttcgccgg acggatagta
    57601 acggcgagac tcttcacgca gatgaatccc cggcagtttc agttttttga tgtagtccgc
    57661 catgtcagag tttacctgac gcgccagata aataaagcgc cctttcgggt tggcgttaat
    57721 gcgggcagaa agctgatcca gcggaatatt gagcgcgtta gccagcgcct tccagcggtc
    57781 accgacgctg ataccgccag cgtcatgcac ttctttcggg tcagcccaaa tcgcttttac
    57841 cggcacgctc accgctaacg ggcgaccaga acggtcagta atcatgccgc gggaggtgga
    57901 aacttgctga acgcgaagag aacgcatgtc gccctctttc accagcatat ccggggagat
    57961 aacttgtaac cacgctacgc gtccgagcag aaaagccagc gccaggagaa tacagccgca
    58021 taacaacgca aaacgccaac tgataaagtt ggcatgttct tcctgacgtt ttggtttctg
    58081 cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat ttttgcacta cgatattttc
    58141 ttgtgacgga tcaacatgct gcatttgcag cttttccgtg gcgatccttt ccacccggct
    58201 atggtcgccg agcgcattct cttcaaggat caggttgcgc cattcaatgt ctaaagcatc
    58261 tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa cgggtatggt gcgccgtggt
    58321 taccacggtc accgccgtca aaataatgca aatgaacagg cagagtggca gcttcccaaa
    58381 tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg cgctcgtggc ttcccatcga
    58441 tcctttaact ttgcttagag cttctgtcac tctgctgatc atgcgttcgt cctctctgca
    58501 atacgcagaa ctgaactacg ggcacgaggg ttctcagcca cctcttcttc gcccggcatt
    58561 aacttgccta gtgctcgcag ctgacggcca cccagttttt tgagctgctc ttcagtcatc
    58621 ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac gcataaaacg tttcacaata
    58681 cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc cacccggggc cagcacgttg
    58741 agcgagcttt ttagcgcctg ctctatctcc tccagttcac tgtttaccca aatgcgcacc
    58801 gcctggaagg tacgggtcgc gggatgttta aacttatctt tcaccggcgt tgcagcagcc
    58861 acgacttccg ccagttcttt ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg
    58921 gcgcgggcaa tgcgtttggc aaaatgctct tcaccatagg ttttcaatac ccaggcgata
    58981 tcggcttctt ctgcggtttg tagccattca gcggctgact gcccacgggt tgggtccata
    59041 cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc gttcagcatc atcaagttgc
    59101 ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct tgccaataag atcgcgctcg
    59161 gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga tggagaagcg cggatcatca
    59221 atagtcttcg caacggcgat agcctgcggg tcgcgatcga tcgccagcaa acgcccctct
    59281 tcgccaagct gcgagaggat cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg
    59341 tagatgccat caggacggat attgaggcca ttaacggctt catccagcag caccgtagta
    59401 tgtttatagt tttccatcat tttatagaga caagtcctgc agtcgctccg ataagtctcc
    59461 ggtagccaac tgctctgcgt cgatatcttc cttgacctgt tgatgccagg ttgtttcatc
    59521 ccacagctca aacttgttga actgtccaac cagcatcact tcttttgtca gcccggcatg
    59581 ttgccgcagt actggcgcga ttaacaatcg acctgcgcca tccatctgac attcgctggc
    59641 atgacctaac agtaggcgct gcacacggcg ctcaaccggg ttcatgctca acagacgcga
    59701 taatttttgc tcgataattt cccattcagg cagggggtaa agcagcaggc acgggtgatg
    59761 aatgtcaatg gtgcaaacca tttgaccggc agcgttctca agcagctgtt cccgataacg
    59821 ggtaggcact gataagcgcc ctttgctgtc gagattgact aacgttgctc cccggaacat
    59881 gccagcctca ccccttattc ccactttgcc ccacaatttc ccactgaaag gagtttacgg
    59941 agcggaggaa aagcttgtca agccagtcac aaggcatacc gaggtaaaaa acccgatgtt
    60001 tacggctaat atcagcgttg tttattttac gtccaacgtg cgaagcaaat taacgttatg
    60061 aatagctgta agaaaaaatc cctcatcgtc atcactgttt taaaatgact cactattttc
    60121 cacagcaaat ataaagtgtc agtttgcgac gcgagcggca ttttaggaca tatcttcccc
    60181 ggttaacagt ccttgttacg tctgtgtggc gcttgatgcc agcgggtttg cgcaaaagca
    60241 aagaaaagtg tttgttaaat cggcaattca tcgccgcatg taacaaaata atgcaaaaac
    60301 gcgctgtcga tatcacttaa ttattgtgaa tttcagctgg tgagttaatt aacgggcggg
    60361 tttaaaacca tttatcaata atagtcagga atagtcttat ttactttaag gatattgat
//
LOCUS       DAJTQQ010000012        59862 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000012 DAJTQQ010000000
VERSION     DAJTQQ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.012"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            546..1835
                     /gene="sdaC"
                     /locus_tag="OO309_000835"
     CDS             546..1835
                     /gene="sdaC"
                     /locus_tag="OO309_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR5846082.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1893..3260
                     /gene="sdaB"
                     /locus_tag="OO309_000836"
     CDS             1893..3260
                     /gene="sdaB"
                     /locus_tag="OO309_000836"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR5846083.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3371..4126
                     /gene="xni"
                     /locus_tag="OO309_000837"
     CDS             3371..4126
                     /gene="xni"
                     /locus_tag="OO309_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR5846084.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4179..5327)
                     /gene="fucO"
                     /locus_tag="OO309_000838"
     CDS             complement(4179..5327)
                     /gene="fucO"
                     /locus_tag="OO309_000838"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR5846085.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5355..6002)
                     /gene="fucA"
                     /locus_tag="OO309_000839"
     CDS             complement(5355..6002)
                     /gene="fucA"
                     /locus_tag="OO309_000839"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR5846086.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6549..7865
                     /gene="fucP"
                     /locus_tag="OO309_000840"
     CDS             6549..7865
                     /gene="fucP"
                     /locus_tag="OO309_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR5846087.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7898..9673
                     /gene="fucI"
                     /locus_tag="OO309_000841"
     CDS             7898..9673
                     /gene="fucI"
                     /locus_tag="OO309_000841"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR5846088.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9782..11201
                     /gene="fucK"
                     /locus_tag="OO309_000842"
                     /pseudo
     CDS             9782..11201
                     /gene="fucK"
                     /locus_tag="OO309_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11203..11625
                     /gene="fucU"
                     /locus_tag="OO309_000843"
     CDS             11203..11625
                     /gene="fucU"
                     /locus_tag="OO309_000843"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR5846089.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11683..12414
                     /gene="fucR"
                     /locus_tag="OO309_000844"
     CDS             11683..12414
                     /gene="fucR"
                     /locus_tag="OO309_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR5846090.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12458..13558)
                     /gene="rlmM"
                     /locus_tag="OO309_000845"
     CDS             complement(12458..13558)
                     /gene="rlmM"
                     /locus_tag="OO309_000845"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR5846091.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13551..13946)
                     /locus_tag="OO309_000846"
     CDS             complement(13551..13946)
                     /locus_tag="OO309_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR5846092.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13965..14882)
                     /gene="gcvA"
                     /locus_tag="OO309_000847"
     CDS             complement(13965..14882)
                     /gene="gcvA"
                     /locus_tag="OO309_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR5846093.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15233..15460)
                     /locus_tag="OO309_000848"
     CDS             complement(15233..15460)
                     /locus_tag="OO309_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR5846094.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15652..16857
                     /gene="csdA"
                     /locus_tag="OO309_000849"
     CDS             15652..16857
                     /gene="csdA"
                     /locus_tag="OO309_000849"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR5846095.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16857..17300
                     /gene="csdE"
                     /locus_tag="OO309_000850"
     CDS             16857..17300
                     /gene="csdE"
                     /locus_tag="OO309_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR5846096.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17351..18157)
                     /gene="tcdA"
                     /locus_tag="OO309_000851"
     CDS             complement(17351..18157)
                     /gene="tcdA"
                     /locus_tag="OO309_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR5846097.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18395..19492)
                     /gene="mltA"
                     /locus_tag="OO309_000852"
     CDS             complement(18395..19492)
                     /gene="mltA"
                     /locus_tag="OO309_000852"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR5846098.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19701..19777
                     /locus_tag="OO309_000853"
     tRNA            19701..19777
                     /locus_tag="OO309_000853"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19735..19737,aa:Met,seq:cat)
     gene            19811..19887
                     /locus_tag="OO309_000854"
     tRNA            19811..19887
                     /locus_tag="OO309_000854"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19845..19847,aa:Met,seq:cat)
     gene            complement(19962..21215)
                     /gene="amiC"
                     /locus_tag="OO309_000855"
     CDS             complement(19962..21215)
                     /gene="amiC"
                     /locus_tag="OO309_000855"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR5846099.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21447..22778
                     /gene="argA"
                     /locus_tag="OO309_000856"
     CDS             21447..22778
                     /gene="argA"
                     /locus_tag="OO309_000856"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR5846100.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22840..24666)
                     /gene="recD"
                     /locus_tag="OO309_000857"
     CDS             complement(22840..24666)
                     /gene="recD"
                     /locus_tag="OO309_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR5846101.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24666..28208)
                     /gene="recB"
                     /locus_tag="OO309_000858"
     CDS             complement(24666..28208)
                     /gene="recB"
                     /locus_tag="OO309_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR5846102.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28201..31089)
                     /gene="ptrA"
                     /locus_tag="OO309_000859"
     CDS             complement(28201..31089)
                     /gene="ptrA"
                     /locus_tag="OO309_000859"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR5846103.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31265..34633)
                     /gene="recC"
                     /locus_tag="OO309_000860"
     CDS             complement(31265..34633)
                     /gene="recC"
                     /locus_tag="OO309_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR5846104.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34646..34996)
                     /locus_tag="OO309_000861"
     CDS             complement(34646..34996)
                     /locus_tag="OO309_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR5846105.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            complement(34981..35388)
                     /locus_tag="OO309_000862"
     CDS             complement(34981..35388)
                     /locus_tag="OO309_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR5846106.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35385..35948)
                     /locus_tag="OO309_000863"
     CDS             complement(35385..35948)
                     /locus_tag="OO309_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR5846107.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35939..36409)
                     /locus_tag="OO309_000864"
                     /pseudo
     CDS             complement(35939..36409)
                     /locus_tag="OO309_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36593..37387)
                     /gene="thyA"
                     /locus_tag="OO309_000865"
     CDS             complement(36593..37387)
                     /gene="thyA"
                     /locus_tag="OO309_000865"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR5846108.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37394..38269)
                     /gene="lgt"
                     /locus_tag="OO309_000866"
     CDS             complement(37394..38269)
                     /gene="lgt"
                     /locus_tag="OO309_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR5846109.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38420..40666)
                     /gene="ptsP"
                     /locus_tag="OO309_000867"
     CDS             complement(38420..40666)
                     /gene="ptsP"
                     /locus_tag="OO309_000867"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5846110.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40679..41209)
                     /gene="rppH"
                     /locus_tag="OO309_000868"
     CDS             complement(40679..41209)
                     /gene="rppH"
                     /locus_tag="OO309_000868"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR5846111.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41894..42583
                     /gene="mutH"
                     /locus_tag="OO309_000869"
     CDS             41894..42583
                     /gene="mutH"
                     /locus_tag="OO309_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR5846112.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42652..43365
                     /locus_tag="OO309_000870"
     CDS             42652..43365
                     /locus_tag="OO309_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5846113.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43503..43721
                     /gene="ygdR"
                     /locus_tag="OO309_000871"
     CDS             43503..43721
                     /gene="ygdR"
                     /locus_tag="OO309_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR5846114.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43829..44869
                     /locus_tag="OO309_000872"
     CDS             43829..44869
                     /locus_tag="OO309_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR5846115.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44902..46092)
                     /gene="lplT"
                     /locus_tag="OO309_000873"
     CDS             complement(44902..46092)
                     /gene="lplT"
                     /locus_tag="OO309_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR5846116.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(46085..48244)
                     /gene="aas"
                     /locus_tag="OO309_000874"
     CDS             complement(46085..48244)
                     /gene="aas"
                     /locus_tag="OO309_000874"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR5846117.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48830..49861
                     /gene="galR"
                     /locus_tag="OO309_000875"
     CDS             48830..49861
                     /gene="galR"
                     /locus_tag="OO309_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR5846118.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49868..51130)
                     /gene="lysA"
                     /locus_tag="OO309_000876"
     CDS             complement(49868..51130)
                     /gene="lysA"
                     /locus_tag="OO309_000876"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR5846119.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51252..52187
                     /gene="lysR"
                     /locus_tag="OO309_000877"
     CDS             51252..52187
                     /gene="lysR"
                     /locus_tag="OO309_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR5846120.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52174..52866)
                     /gene="ygeA"
                     /locus_tag="OO309_000878"
     CDS             complement(52174..52866)
                     /gene="ygeA"
                     /locus_tag="OO309_000878"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5846121.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52995..54413)
                     /gene="araE"
                     /locus_tag="OO309_000879"
     CDS             complement(52995..54413)
                     /gene="araE"
                     /locus_tag="OO309_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR5846122.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54728..55489)
                     /gene="kduD"
                     /locus_tag="OO309_000880"
     CDS             complement(54728..55489)
                     /gene="kduD"
                     /locus_tag="OO309_000880"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR5846123.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(55519..56355)
                     /gene="kduI"
                     /locus_tag="OO309_000881"
     CDS             complement(55519..56355)
                     /gene="kduI"
                     /locus_tag="OO309_000881"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR5846124.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56642..57823)
                     /locus_tag="OO309_000882"
     CDS             complement(56642..57823)
                     /locus_tag="OO309_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR5846125.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            58078..59307
                     /locus_tag="OO309_000883"
                     /pseudo
     CDS             58078..59307
                     /locus_tag="OO309_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT        14778 a        16061 c        14579 g        14444 t
ORIGIN      
        1 ccggtgacgt ttgttgccgg gttttctcgt ttttggtcac ttactcatca actcatttca
       61 tttgttatat gaatgtttct taccaccctc acgcgacaaa tatcatcaca gttaatatgt
      121 catacaattt atgttgcaac acaaacgttt ccctattttc ataaaccgta ttttatcgct
      181 atgaaaaaga aattatcgcc atgattaact aaaagtattg attttttcag ttcaacctac
      241 atatattgcg cgccccggaa gaagtcagat gtcgtttaat gggcaaatat tgcccttaaa
      301 ttctctttta cttttgattt acagagtaaa gcgttgggat aatctatctt ccaagcagat
      361 tattgtattt gggatcaaga tcactgatag atacataact tgtgtgtatc tttccgccct
      421 caaattatta cggcggtaaa tgattaagcc atcgccgata gacagatttc atttttacgg
      481 tcaggcacct tcccgggctg aactggctaa aagctgaatt atttgcattc ctccaggaga
      541 aatagatgga aacgactcaa accagcacga ttgcgtcgaa agactctcgt agtgcctggc
      601 gcaagacaga caccatgtgg atgctgggcc tttacggcac ggcaatcggc gcgggcgtgc
      661 tgttcctgcc aatcaacgcc ggtgttggcg gtatgatccc gctgatcatc atggctatcc
      721 ttgcgttccc gatgacgttt tttgctcacc gcggcctgac tcgcttcgta ctgtctggta
      781 aaaacccggg cgaagacatc accgaggttg tagaagaaca ctttggtatt ggcgcaggta
      841 aactgattac cctgctctac ttcttcgcta tctacccgat cctgctggtt tatagcgtgg
      901 caatcaccaa taccgttgaa agcttcatgt ctcaccagct gggtatgacg ccaccgccgc
      961 gtgcgattct gtcgctgatc ctgatcgtgg gtatgatgac catcgttcgc tttggtgagc
     1021 agatgatcgt taaagcgatg agtattctgg tattcccgtt tgttggcgta ctgatgctgc
     1081 tggctctgta cctgatcccg cagtggaacg gcgcagcact ggaaacgctg tctctggaca
     1141 ctgcatctgc aaccggaaac ggtctgtgga tgaccctgtg gctggcaatt ccggtaatgg
     1201 tgttctcgtt caaccactct ccgatcatct cttctttcgc cgttgcgaag cgtgaagagt
     1261 acggcgatat ggcagaacag aaatgctcga agatcctggc attcgcacac atcatgatgg
     1321 tgctgaccgt aatgttcttc gtcttcagct gcgtactgag cctgactccg gcagacctgg
     1381 ctgcggctaa agagcagaac atctcgattc tgtcttacct ggctaaccac tttaacgcac
     1441 cggttatcgc gtggatggct ccgattatcg cgattatcgc tatcaccaaa tccttcctcg
     1501 gccactacct gggcgcacgt gaaggcttca acggtatggt gattaaatct ctgcgtggta
     1561 aaggtaagtc tatcgaaatc aacaagctga accgtatcac tgcgctgttc atgctggtaa
     1621 cgacctggat tgttgccacc ctgaacccga gcatcctggg tatgattgaa accctgggcg
     1681 gcccaatcat cgcgatgatc ctgttcctga tgccgatgta cgcaattcag aaagtaccgg
     1741 caatgcgtaa gtacagcggt cacatcagca acgtattcgt tgtcgtgatg ggtctgattg
     1801 caatctccgc aatcttctac tctctgttca gctaagtcct ttcgcgccgc tttcgggcgg
     1861 cgcttcctcc gttttaacgc gatgtatttc ctatgattag cgtattcgat attttcaaaa
     1921 tcggcattgg cccttccagt tctcataccg ttggaccaat gaaagcgggt aaacaattta
     1981 ccgacgatct gattgcccgt aacctgctta aagacgtgac ccgcgtggtg gttgacgtgt
     2041 acggctcgct ctctctgact ggtaaaggcc accacacaga tatcgccatt attatgggcc
     2101 tggcgggtaa cctgccggat accgtggata tcgattccat ccccggtttt attcaggatg
     2161 tgaatactca tggtcgcctg atgctggcaa acggtcagca tgaagtggag ttcccggttg
     2221 atcagtgcat gaacttccat gcagacaacc tttctctgca tgaaaacggt atgcgcatta
     2281 ccgcgctggc gggcgataaa gtcgtttaca gccagactta ctactctatt ggcggtggct
     2341 ttatcgttga tgaagagcat tttggccagc aggatagcgc accggttgaa gttccttatc
     2401 cgtacagttc agcagccgat ctgcaaaaac attgtcagga aaccgggctg tcactctctg
     2461 gcctgatgat gaaaaacgag ctggcgctgc acagtaaaga agagctggaa cagcacctgg
     2521 cgaacgtctg ggaagtcatg cgtggcggta ttgagcgcgg tatttccacc gaaggcgtgt
     2581 tgcctggcaa actgcgcgtt ccacgccgtg ctgcggcact acgccggatg ctggtcagcc
     2641 aggataaaac caccactgac ccgatggcgg ttgttgactg gatcaacatg tttgcactgg
     2701 cagtgaacga agagaacgct gctggcggtc gcgtggtgac tgcgccgact aacggtgcgt
     2761 gcgggattat cccggctgtg ctggcgtact acgataagtt tatccgcgaa gtgaacgcta
     2821 actcactggc tcgttacctg ctggtagcca gcgccattgg ttctctttat aagatgaacg
     2881 cgtcgatttc tggtgccgaa gtgggttgcc agggtgaagt tggcgtggcg tgctctatgg
     2941 cggcagctgg tctggcagaa ctgttaggcg caagcccggc gcaggtttgc atcgcagcgg
     3001 aaatcgcgat ggagcacaac ctcggtctga cttgtgaccc ggtcgcgggg caggttcagg
     3061 taccgtgcat tgagcgtaac gccattgcag cagtgaaagc ggtgaacgcc gcgcgtatgg
     3121 cactgcgccg taccagcgaa ccgcgcgtct gcctcgataa agttatcgaa accatgtacg
     3181 aaacaggtaa agacatgaac gccaagtacc gcgaaacctc tcgcggcggc ctggcgatga
     3241 agatcgttgc ctgcgattaa tctctcccag aggcctcgtt ttgcgaagcc tcttcctgtt
     3301 ttctcaacca gccatagcct gttccggcat cgaatgttac ccttatcgcc tgatctttaa
     3361 gggggttatc gtggctgttc atttgcttat tgtcgatgca ctgaatctta tccgtcgcat
     3421 tcatgccgtt cagggttcgc cctgtgtcga aacctgccag catgcgctcg atcagctcat
     3481 tatgcacagc cagccaaccc acgcggtcgc cgtttttgat gatgaaaacc gcagtagcgg
     3541 ctggcgtcat cagcgtttac cagattacaa agcggatcga ccgccaatgc cggaagagtt
     3601 gcacgacgag atgcctgcat tacgcgccgc ctttgagcaa cgcggcgtcc cgtgctggtc
     3661 agccagcggc aacgaagccg atgacttagc cgccacactg gcggtcaaag tgacacaggc
     3721 cgggcatcag gcaacgattg tttcgacaga taaaggctac tgtcagttac tttcaccgac
     3781 attacgtatt cgtgattact tccagaaacg ttggctggat gcgccattta tcgataaaga
     3841 atttggcgtt caaccgcagc agttgcccga ttactgggga cttgcgggga tcagcagttc
     3901 aaaggtaccg ggtgttgcgg gaatcggacc aaaaagcgcc acgcagctgc tggtcgagtt
     3961 tcagagtctg gaagggatat atgagaatct ggatgcggtt gccgaaaagt ggcgcaaaaa
     4021 attagaaacc cataaagaga tggcgtttct gtgccgcgat attgcccgct tacaaaccga
     4081 tttgcatatc gacggcaatt tacagcaatt gcggttggta cggtaacggc gagccggata
     4141 cgccgcaaat cgtatccggc attatcacat cagcgcattt accaggcggt atggtaaagc
     4201 tctacaatat cctcaagcgt tgcttcacgc gggttgccac cggtacaaac atcatccagt
     4261 gccgcctgag ccagtgccga aatgtcttcc ttgcgtacac caacatcacg caaatgtggc
     4321 ggaataccga catcacggtt gagggcaaac accgcttcaa cagcggcatt acgcgcccct
     4381 tccaggctca taccttccac tttcacgccc ataacgcgcg cgatatcgcg gtacttctca
     4441 ccggtaaagt cagcgttata gcgcatgaca tgcggtaaca ggatggcgtt cgcaacaccg
     4501 tgtggagtgt tataaaacgc gcccagtgga tgcgccatac catgcaccaa ccctaaccca
     4561 acattcgaga agcccatacc cgcaacatac tgcccgagcg ccatttcttc tccggcatcc
     4621 ttatcaccag caaccgatcc tcgcagcgcc ccagcaatga tttcaatcgc tttaatgtgc
     4681 agtgcatcgg ttagtgccca cgcgccacgg gtaatatacc cctcaatagc atgagtgagc
     4741 gcatcaacac ccgtcgcggc tttcagcgcc ggaggcatac catccatcat gtcagcatca
     4801 ataaacgcca cctgcgggat atcatgcgga tcaacgcaaa caaacttgcg ccgtttttct
     4861 tcgtcagtga tcacgtagtt aatggtcact tctgccgcag tacctgctgt ggtggggatt
     4921 gccagaatcg gtacactggg tttattggtc ggggaaagcc cttccaggct acgcacatcg
     4981 gcaaactccg ggttgttgct gataatgcca attgctttac aagtatcctg tggagaacca
     5041 ccaccaatag cgatcaggta atccgcgccg ctattctgga atacaccgag cccttctttg
     5101 acgacagtaa ttgttgggtt gggcactacg ccgtcgtaaa tcgcccatgc cagccctgca
     5161 gcatccatct tatcggtcac tttcgccacc acgccgcatt gcaccagcgt tttatcggtg
     5221 acgatcagcg ccttctgata accacggcgt ttcacctcat cggttaaagc cccaacagca
     5281 ccccgaccaa accatgccgt ttcgttcaga atcattctgt tagccatcat ccttctcctt
     5341 gttgctttac gaaattactc ttcaatccgt aacccatagg ttttgaattt ctccagcact
     5401 acggcaatct cttcatcgct cagcactggc accgggtccg taatcgccag ggtcgtcagg
     5461 taaagttgcg ccagcacttc aacttcatgc gccagccata acgctttttc cagattcacc
     5521 tcacaagcga taagcccatg atgttgtaac aaagttgcct tacgattttt gagagccagc
     5581 gcaacatgtt cagaaagttc gcgtgttcca aaggtcgcat aaggcgcgca aggaatagaa
     5641 ttaccgccag ccgccgcaat catgtagtga atagcgggga tcgatcggtt aagaatggaa
     5701 actgccgtgc aatgaacggc atgattgtga acaaccgcgt tggcatccgg tctgctttga
     5761 taggctgcca tatggaaacg ccattcgctt gaggggagct ttccttcctc atgtttaccg
     5821 ttgccatcaa taaagacaat atgcgactcc gtcagttttt catatggaat gcctgtaggc
     5881 gtaatcagca tcccatcctg ataacgtaca ctgacgttcc ccgctgtccc ctggttcagt
     5941 cccaggcggg tcatttccag gcaagtgtca ataatctgac gagcaagttt atttcgttcc
     6001 attagctacc tctctctgat tcaaaacagg gcaataatgt tgttcctttc acactattga
     6061 attagccgtt taattaccca ccatcttctt cctgattaac aagaaagaaa ttcacaagct
     6121 tatattttgt gacctagctc aactaatcac agtaaataac tgcaagttct ctttttataa
     6181 ccccgttaaa aatgaccgct cttaaaaata tttatcaaaa cggtcatttt tccatttccc
     6241 ctcgccagaa atgcccgttt tcggcacaaa caattaatac ggtcatctgg tttgtgtttt
     6301 ttatgattta ttttctgaaa cgggcatgaa atttcgatta ttaaagtgat ggtagtcaca
     6361 taaagtcacc ttccagctaa taagtgtgac cgccgtcata ttacagagcg ttttttattt
     6421 gaaaatgaat ccatgagttc atttcagaca ggcaaatatt caccgatatg aagcccgaac
     6481 tcgctggttt tgcacttttg aaaacataac cgattacgtg cttaagcttc tgaacctaag
     6541 aggatgctat gggaaacaca tcaatacaaa cgcagagtta ccgtgcggta gataaagatg
     6601 cagggcaaag cagaagttac attattccat tcgcgctgct gtgctcactg ttttttcttt
     6661 gggcggtagc caataacctt aacgacattt tattacctca attccagcag gcttttacgc
     6721 tgacaaattt ccaggctggc ctgatccaat cggcctttta ctttggttat ttcattatcc
     6781 caatccctgc tgggatattg atgaaaaaac tcagttataa agcagggatt attaccggat
     6841 tatttttata tgccttgggc gctgcattat tctggcccgc tgcagaaata atgaactaca
     6901 ccttattttt agttggccta tttattattg cagccggatt aggttgtctg gaaactgccg
     6961 caaacccttt tgttacggta ttagggccgg aaagcagtag tcacttccgc ttaaatcttg
     7021 cgcaaacatt taactcgttt ggcgcaatta tcgcggttgt ctttgggcaa agtcttattt
     7081 tgtctaacgt gccacatcaa tcgcaagacg ttctcgataa aatgtctcca gagcaattga
     7141 gtgcgtataa acacagcctg gtattatcgg tacagacacc ttatatgatc atcgtggcta
     7201 tcgtgttact ggtcgccctg ctgatcatgc tgacgaaatt cccggcattg cagagtgata
     7261 atcacagtga cgccaaacaa ggatcgttct ccgcatcgct ttctcgtctg gcgcgtattc
     7321 gtcactggcg ctgggcggta ttagcgcaat tctgctatgt cggcgcacaa acggcctgct
     7381 ggagctattt gattcgctac gctgtagaag aaattctagg tatgactgca ggctttgccg
     7441 ctaactattt aaccggaacc atggtgtgct tctttattgg tcgtttcacc ggtacctggc
     7501 tcatcagtcg cttcgcacca cacaaagtcc tggccgccta cgcattaatc gctatggcac
     7561 tgtgcctgat ctcagccttc gctggcggtc atgtgggctt aatagccctg actttatgca
     7621 gcgcctttat gtcgattcag tacccaacaa tcttctcgct gggcattaag aatctcggcc
     7681 aggacaccaa atacggttcg tccttcatcg ttatgaccat tattggcggc ggtattgtca
     7741 ctccggtcat gggttttgtc agtgacgcgg cgggcaacat ccccactgct gaactgatcc
     7801 ccgcactctg cttcgcggtc atctttatct ttgcccgttt ccgttctcaa acggcaacta
     7861 actgaacata ttttccgaat aaagtgagga atctgtaatg aaaaaaatca gcttaccgaa
     7921 aattggtatc cgcccggtta ttgacggtcg tcgcatgggt gttcgtgagt cgcttgaaga
     7981 acaaacaatg aatatggcga aagctacggc cgcactgctg accgagaaac tgcgccatgc
     8041 ctgcggagct gccgtcgagt gtgtcatttc cgatacctgt atcgcgggta tggctgaagc
     8101 cgctgcttgc gaagaaaaat tcagcagcca gaatgtaggc ctcaccatta cggtaacgcc
     8161 ttgctggtgc tatggcagtg aaaccatcga catggatcca acccgcccga aagccatttg
     8221 gggctttaac ggcactgaac gccccggcgc tgtttacctg gctgcggctc tggcagctca
     8281 cagccagaaa ggcatcccgg cattctccat ttacggtcat gacgttcagg atgccgatga
     8341 cacatcgatt cctgccgatg ttgaagaaaa actgctgcgc tttgcccgcg ccggtttggc
     8401 cgtcgccagc atgaaaggta aaagctatct gtcgctgggc ggcgtttcga tgggtatcgc
     8461 cggttctatt gttgatcaca acttctttga atcctggctg ggaatgaaag tccaggcggt
     8521 ggatatgacc gaactgcgtc gccgtatcga tcagaagatt tacgacgaag ccgaactgga
     8581 aatggcactg gcctgggctg ataaaaactt ccgctatggc gaagatgaaa ataacaaaca
     8641 gtatcaacgc aatgccgagc aaagccgcgc agttctgcgc gaaagtttac tgatggcgat
     8701 gtgtatccgc gacatgatgc aaggcaacag caaactggcc gatattggtc gcgtggaaga
     8761 atcacttggc tacaacgcca tcgctgcggg cttccagggg caacgtcact ggaccgatca
     8821 atatcccaat ggtgacaccg ccgaagcgat cctcaacagt tcatttgact ggaatggcgt
     8881 gcgcgaaccc tttgtcgtgg cgaccgaaaa cgacagtctt aacggcgtgg caatgctaat
     8941 gggtcaccag ctcaccggca ccgctcaggt atttgccgat gtgcgtacct actggtcacc
     9001 agaagcaatt gagcgtgtaa cggggcataa actggacgga ctggcagaac acggcatcat
     9061 ccatttgatc aactcaggtt ctgctgcgct ggacggttcc tgtaaacaac gcgacagcga
     9121 aggtaacccg acgatgaagc cgcactggga aatctctcag caagaggctg acgcttgcct
     9181 cgccgctacc gaatggtgcc cggcgatcca cgaatacttc cgtggcggcg gttactcttc
     9241 ccgcttcctt accgaaggcg gcgtcccgtt caccatgact cgtgtcaaca tcatcaaagg
     9301 cctgggaccg gtactgcaaa tcgcggaagg ctggagcgtg gaattgccga aggatgtgca
     9361 tgacatcctc aacaaacgca ccaactcaac ctggccaacc acctggtttg caccgcgcct
     9421 cactggtaaa gggccgttta cggatgtgta ttcggtaatg gcgaactggg gtgccaacca
     9481 cggagttctg accatcggcc acgttggcgc agactttatc acccttgcct ccatgctgcg
     9541 tatcccggta tgtatgcaca acgttgaaga gaccaaagtg tatcgtcctt ctgcctgggc
     9601 tgcgcacggc atggatattg aaggccagga ttaccgcgct tgccagaact acggtccgtt
     9661 gtacaagcgt taatacatta caccctgatg tgatgcccgg tcgctccggc taccgggcct
     9721 gaacaagcaa gagtggttag ccggataaac aatgttatcc ggctatattg caggagcgat
     9781 tatgaaacaa gaagttatcc tggtactcga ctgtggcgcg accaatgtca gggccatcgc
     9841 ggttaatcgg cagggaaaaa ttgttgcccg cgcctcaacg cctaatgcca gcgatatcgc
     9901 gatggaaaac aacacctggc accagtggtc tttagactgc cattttgcaa cgctttgctg
     9961 attgctgtcg gcaaatcaat agtgaactga ctgaatgcca catccgcggt atcgccgtca
    10021 ccacctttag tgtggatggc gctctggtag ataagcaagg caatctgctc tatccgatca
    10081 ttagctggaa atgtccgcga acagcagcgg ttatggacaa tattgaacgg ttaatctccg
    10141 cacagcggtt gcaggctatt tctggcgtcg gagcctttag tttcaatacg ttatataagt
    10201 tggtgtggtt gaaagaaaat catccacaac tgctggaacg cgcgcacgcc tggctcttca
    10261 tttcgtcgct gattaaccac cgtttaacca gcgaattcac tactgatatc acgatggccg
    10321 gaaccagcca gatgctggat atccagcaac gcgatttcag tccgcaaatt ttacaagcca
    10381 ccggtattcc acgccgactc ttccctcgtc tggtggaagc gggtgaacag attggtacgc
    10441 tacagaacaa cgccgcagca atgctcggct tacccgttgg cataccggtg atttccgcag
    10501 gtcacgatac ccagttcgcc ctttttggcg ctggtgccga acaaaatgaa cccgtgctct
    10561 cctccggtac atgggaaatt ttaatggttc gcagcgccta ggttgatact tcgctgttaa
    10621 gtcagtacgc cggttccacc tgcgaactag atagccaggc atggttgtat aacccaggta
    10681 tgcaatggct ggcatccggc gtgctggaat gggtgagaaa actgttctgg acggctgaaa
    10741 caccctggca aatgttgatt gaagaagctc gtctgatcgc acctggcgcg gatggtgtga
    10801 aaatgcagtg tgatttattg tcgtgtcaga acgctggctg gcaaggagtg acgcttaata
    10861 ccacgcgggg gcatttctat cgcgcggcgc tggaagggtt aactgcgcaa ttacagcgca
    10921 atctacagat gctggaaaaa atcgggcact ttaaagccta tgaattattg ttagtcggtg
    10981 gaggaagtcg caacacattg tggaatcaga ttaaagccaa tatgcttgat attccggtaa
    11041 aagttctcga cgatgccgaa acgaccgtcg caggagctgc gctgttcggt tggtatggcg
    11101 taggggaatt taacagcccg gaagaagccc gtgcacagat tcattatcag taccgttatt
    11161 tctacccgca aactgaacct gaatttatag aggaagtgtg aaatgctgaa aacaatttcg
    11221 ccgttaattt ctcccgaact attgaaagtg ctggcagaga tgggacatgg agatgaaatt
    11281 attttttccg atgctcactt tcccgcccat tcgatggaac cgcaggtgat ccgcgctgat
    11341 ggcctgttgg tgagcgactt gctccaggcg attatcccgt tatttgagct ggacagttat
    11401 gccccgccgc tggtgatgat ggcggcggta gagggtgaca ctctcgatcc tgaagtagaa
    11461 cgacgttacc gtaatgcgct ttcactacaa gccccgtgtc ctgacatcat ccgcatcaat
    11521 cgttttgcgt tttatgaacg ggcgcaaaaa gcctttgcga tcgttatcac aggcgaacga
    11581 gcgaagtacg ggaatattct tttaaaaaaa ggggtaacac cgtaatctca taccggtacg
    11641 cccgcatgac gcgggcggtt atcgaatgat ggggtgaaaa atatgaaagc ggcacgccag
    11701 caagcgatag tcgacctgct gctgaaccat accagcctga ccacggaagc tctctctgaa
    11761 cagctaaagg tcagtaaaga aaccattcgt cgcgatctca atgaattaca gacgcagggt
    11821 aaaattctgc gcaatcatgg acgcgctaaa tatatccacc gtcaaaatca agacagtggc
    11881 gatccctttc acatcaggct gaaaagccat tatgcgcata aagcagatat cgcgcgcgag
    11941 gcgctcgcgt ggattgaaga agggatggtg atagccttag acgccagttc aacttgctgg
    12001 tatctggcac gccagttgcc tgacatcaac attcaggtct tcaccaatag ccatccgatt
    12061 tgccatgaac tcggtaaacg cgaacgcatt caactgatca gttccggcgg cacacttgag
    12121 cgcaaatatg gctgttacgt caatccctcg ctgatttccc aacttaaatc gctggaaatc
    12181 gatctgttta ttttttcttg tgaagggatc gatagcagcg gcgcactgtg ggactccaat
    12241 gcgatcaacg ctgattacaa atcgatgcta ttaaaacgtg ccgcgcaatc gttgttattg
    12301 attgataaaa gtaaatttaa tcgttcaggg gaagcccgca tcgggcatct ggatgaggta
    12361 acgcacatta tttctgatga gcgccaggtt gcaacttctt tggtaacagc ctgacggaaa
    12421 gggtagcagg ccggagacga cgccccggcc ttgcctgtta tcgctcgtcg cgacgaccac
    12481 ccaccgcagc ccagatgcgg cggacgtgca ccgtcacttc ttcgcgatcg tgatacaact
    12541 gccgcgcctg aatctgagca tttatgccat gttcatcaag ctgtgcctga atatacgcca
    12601 gattgtgtga tacttcttcg tagcgttttt tcatcggcag tttgagattg aaaatggttt
    12661 cacggcacca gccattaacc agccactgcg ccatcaatgc cgcaacttta gccggttttt
    12721 caaccatatc gcataccatc caggagatat tgctgcgtgt cggacggaat ttgaaaccgt
    12781 cttcccgcag ccacgtcacc tgcccggtat ccatcagact ttgcgccatc gggccgttgt
    12841 cgacggaata aacccacatg ttgcgcttca ccagttggta ggtccagccg ccagggcaag
    12901 cgcctaaatc caccgcccac atcccgttcg ccaggcgttc atcccactca tccgcaggaa
    12961 taaacacatg aaatgcctct tccagtttga gcgtggaacg actcggcgcc tctgccggaa
    13021 atttcaggcg cggaatgccc atatagaacg gcgaattatt gttgctgtat gagtaaccgg
    13081 tatagcagca tcctggtgca ataaagaata catgcacaac cggacgcttc ggcgtttcat
    13141 agttcgccag caccccagca tcgcgcaagg cagcgcgtag cggaacagtg aacttacggc
    13201 agaatttcag caactctttg ctttcgttgg tatcggcaac ttcaacacgc agttcaccgc
    13261 ccttctctac tacaccctgt aacatgccga caatgggggt aatacgatct tctggcggca
    13321 aatgctgcag gagttccccc accacaaacc actggcgggc aaaaattaat gaactgaacg
    13381 gcagctcacg gattaactta tcgccatcat caggttgata acattcataa atgacataac
    13441 ccgcattctc tttcacgcgg gcaaaaccga aaatttcccg ctggccggct ttatcggtaa
    13501 tttctgcggc gcactctttt tcaaagcccg gacggcacag caatacaacc ttattcatga
    13561 cttacgccct tgcgctttaa acggatagca ccaactaaca ttaacgccca gcccgcaagg
    13621 aagctcacgc cgccaaccgg tgtgacaaac gcccacaaac gcagatggga cagcgccagg
    13681 caataaaggc tgccgctgaa cagcaccgtg cctaacgcga ggaaaacgct actccagtaa
    13741 aaccagatac tgatgcgacg ctgcattgcc accgccagac ctaagatcgc cagcgtatga
    13801 aacgcctggt attcgagacc ggtttggatc cagcccatct caacggcccc catggtttta
    13861 cttaacacat gcgcaccaaa agcgcccagt gccacaaaaa tgaagccgct aatggcggcg
    13921 aaaatcagca taaaacggct ggtcatggtc gtaccctacg taaattattg ttcataacga
    13981 aagcggaatt tttcttgttc agcagcggct ttcgccagga tccattggcg aaaggcggct
    14041 attttaccca gttctgcctg gctgtcatga caaaccagat aaaaagcatt tttactgacc
    14101 agaacatcat taaacgggca aacaagacgt ccggcctcga tttcagattg cgccatcacg
    14161 ttatttgcca gcgccactcc ctgcccgtga atagccgctt gcagcaccat ggcgctatga
    14221 ctaaaaattg gcccttgctg aacgttgata tgattaaacc ccaactgtcg ggtatatgtc
    14281 tgccagtcac ggcgtgacgc atcatgtaat aacgtatgtt tagccagatc ttccggggtc
    14341 ttcaagggtt tttcgcctgt cagcagtagc ggcgaacaca ccggcaataa atattcggcg
    14401 tacagttttt ccacccgtag ccccggccag ttgccccgac cataaaatat cgccacatca
    14461 acatcatccg ccagcttatc ttcctgacga tcaaccgcct ggattcgaac gtcaattccc
    14521 ggataagctg aattaaagct ggaaagtcgc ggaaccaacc aatgaatggc gaaactgggg
    14581 agtaaactga ccgtcaacgc ccccttggcg ctacgggcct ggagtttacg cgtcgcttcg
    14641 gttaattgcg aaaatatctc tttgatatcg aggaaatagc tttgcccttc ctcggtcagc
    14701 aggagtgaac gattacggcg gcggaacagt tttagcccca aaaaatcctc aagagacttg
    14761 atttgatgac ttactgcggc ctgggtcaca aaaagctctt ctgctgcgcg agtaaaactt
    14821 aaatggcgtg ctgcggcatc aaaaactcgt aaggcattta gcggtggtaa tcgtttagac
    14881 atggctttta aactttgatg ttaaatgaat ttaacaatta gatcacactg tgtaacctat
    14941 tagttttttt aatctgagcc attataaatt gtccgttgag cttctaccag caaataccta
    15001 tagtggcggc acttcctgag ccggaacgaa aagttttatc ggaatgcgta ttctggtgaa
    15061 cttttggctt acggttgtga tgttgtgttg ttgtgtttgc aattggtctg cgattcagac
    15121 catggtagca aagctacctt ttttcacttc ctgtacattt accctgtctg tccatagtga
    15181 ttaatgtagc accgcctaat tgcggtgctt ttttttacct tgcgatcgcg aattactgtt
    15241 ccagttcgac catctctttc acgtcagtac ggttgatctg ctgtttgttg ccattagcgt
    15301 ctttatacga aatcataccg atatcgttat cagtctgtgg tttgccgtca gagacgatgg
    15361 tacgtccgtc attggtgtgc atcacatagt tcgaaccgga acaggcgctc agggcaaaag
    15421 tcagcataca ggcagaaata attgcggcag tctttttcat catcttctcc ttaaaacgat
    15481 taataaatct taatagtatt gattaacagg ctaaaattaa cgcctaacac tattcagcat
    15541 atgttactta cgcggcttcg ccaggatatc cagataattc tgatggttag cactctcctt
    15601 gtatcaaagt gaattttgcg tcacgatcgg tgcatcaagc cgaggagtac catgaacgtt
    15661 tttaatcccg cgcagtttcg cgcccagttt cccgcactac aggatgcggg cgtctatctc
    15721 gacagcgccg cgaccgcgct taaacctgaa gccgtggttg aagccactcg acagttttac
    15781 agcctgagcg ccggaaacgt ccatcgcagc cagtttgccg aagcccaacg cctgaccgcg
    15841 cgttatgaag ctgcacgaga gaaagtggcg caattactga atgcaccgga tgataaaact
    15901 atcgtctgga cgcgcggcac cactgaatcc atcaacatgg tggcacaatg ctatgcgcgt
    15961 ccgcgtctgc aaccgggcga tgagattatt gtcagcgtgg cagaacacca cgctaacctc
    16021 gtcccctggc tgatggtcgc ccaacaaact agagccaaag tggtgaaatt gccgcttaat
    16081 gcgcagcgac tgccggatgt cgatttgttg ccagagctga ttactccccg tagtcggatt
    16141 ctggcgttgg gtcagatgtc gaacgtgact ggcggttgcc cggatctggc gcgagcgatt
    16201 acctttgctc attcagccgg gatggtggtg atggttgatg gtgctcaggg gacggtgcat
    16261 ttccccgcgg atgttcagca actggatatc gatttctatg ctttctctgg tcacaaactg
    16321 tatggcccga cgggtatcgg cgtgctgtat ggcaaatcag aactgctgga agcgatgtcg
    16381 ccctggctgg gtggcggcaa aatgattcac gaagtgagtt ttgacggttt caccactcaa
    16441 tctgcgccgt ggaaactgga agcaggaacg ccaaatgtcg ctggcgtcat aggattaagc
    16501 gcggcgctgg aatggctggc agattacgat atcaaccagg ccgaaagctg gagccgtagc
    16561 ttagcaacgc ttgcagaaga agcgctggcg aaacgtccag gctttcgttc attccgctgc
    16621 caggattcca gcctgctggc ctttgatttt gctggcgttc atcacagcga tatggtgacg
    16681 ctactggcgg agtacggtat tgccttgcgg gccgggcaac attgcgctca gccgctactg
    16741 gcagaattag gcgtaaccgg cacactgcgc gcctcatttg cgccatataa tacaaagagt
    16801 gatgtggatg cgctggtgaa tgccgttgac cgcgcgctgg aattattggt ggattaatga
    16861 caaacccgca attcgccgga catccgttcg gcacaaccgt aaccgcagaa acgttacgca
    16921 ataccttcgc accgttgtcg caatgggaag ataaatatcg ccagttgatc atgctgggga
    16981 aacagcttcc ggctttgcca gacgagttaa aagcgcaggc taaagagatt gccggatgcg
    17041 aaaaccgcgt ctggctggga tataccgtgg ctgaaaacgg caaaatgcac ttctttggca
    17101 acagcgaagg gcgcattgtg cgcggcctgt tggcggtgtt gttgactgcc gttgagggga
    17161 aaaccgccgc cgaattacag gcacagtcgc cgctggcatt gtttgatgag ctgggattac
    17221 gtgcgcagct tagcgcctca cgcagccagg ggttaaatgc gttaagcgag gcgattatcg
    17281 ctgcgacgaa gcaggtttaa gcacagaacc gggtcggata agacgttcgc gtctcatccg
    17341 acctgattgt ttaaccctga cgcgccgctt tcgccatcat cttcttcagc gcatgagaaa
    17401 ccgcaacaaa cccaaaggtg gcggtcacca tcgttgccgc gccaaatcca gatgcacaat
    17461 ccatccgctt cggcccttct gccgtggctt tcatcgcaca caccgtaccg tctgactgcg
    17521 ggtacaccag cgcttcagta gaaaacacgc aatccacgcc gagcttacct ttactgtttt
    17581 tcactacgcc aaaatcgctt ttcaggcgct cgcgcaactt cgccgccagc ggatcctgaa
    17641 tcgttttcgc cagatcggta acctgaatct gcgtcgggtc aatctgccca cccgcgccac
    17701 cggtcgtgac cagcgggatt ttattgcgcc gacaataagc aatcagcgcc gctttgggcc
    17761 gtacactatc aatggcatca atcacgtacg aataaccggc actcatatat tgcgctacgt
    17821 tatccggcgt cacgaaatca tccaccaccg ttacacggca ctccgggtta atctggcgaa
    17881 tgcgctccgc catgacttcc gcttttgcca gcccgacgtt atcgcgcaga gcgtgaattt
    17941 gccgattggt attggtgacg cacacatcat ccatatcgat aagcgtgatt gcgccaatcc
    18001 ccgtgcgcgc cagcgcttcc gccgcccagg aaccgacacc accgatacca accacacaaa
    18061 tatgcgcgtc agcaaacagt tgcaacgctt tttcaccata cagacgcgct gtgccaccaa
    18121 aacgctgacg ccatgcatca ctaattacca cagacatagc acctcagaat taaaaagggc
    18181 gaagttatcc ctcaccctaa acgcataaat gcctgatgcg ctacgcttat caggcctaca
    18241 taactcctgc aatattttgg atttgcatga tttgtaggcc ggataaggcg ttcacgccgc
    18301 atccggtatg aacaacgcgc acttagtcaa taatcaggat gtgaggttac ccctcccctg
    18361 tcatatccgt aaaaacggca tacagaatat cacatcagcc gctaaagacg ttacctgcgc
    18421 ccggagccgt tttcagcacc caaacccgtc catagtggtt gtaccaacct gcgcggtgtc
    18481 cggcttccgg cccgatccct tgatagatat cgaagtgttg gcctttgatt gcgcccccga
    18541 catccaacgc caccatcaga cgcaattcgt actgaccgtt aaatttgccg ttattatcca
    18601 gcagcggcac ttctgccagc aaggtagtac ctggcggaat aattgaacga tcggaggcaa
    18661 cagaagcacg gccaaccagc ggcaccgcac ttgccccttt caccggtgca aaagattgcg
    18721 gtttaaagaa gacgaaagac gggttctgtt ccagcagctc gcgaacctcg gcttcactgt
    18781 gtgtttcgcc ccaatgacga atcgcctgca tcgacatatc ttcttttttc acttcgccac
    18841 ggtcgatcag caccttacca atgctgcgat aggcatgacc gtttttccct gcatagctga
    18901 aaaagttaag cggactgcca tcaccaaagt cgatataccc actaccctgc acatccataa
    18961 tgaagttatc catcagagag ttgctgtaag cgaggatgta tttatcgccc aatgccccgg
    19021 cgtagatctc cgcacgagac ggcagacgac cacgttttgg cggcatacgg taaataggat
    19081 actggaactc gccctggcgg gtatggcgcg cctgaattac cggcgtgtaa tagcctgtaa
    19141 actgcacgtt accatagttg tcggcacctt ccatctgcca ggcatcaatg ccgaactggc
    19201 gcatattgcg ggtatcaccg cctgcgcgca gccactcttg caccgcgtta taaacattac
    19261 tctggttgcc atacagacgc ggtgacgaat tacggatatg gttaatttgc tcggcaaaat
    19321 caccggcgtt aatcggcgcg ccaacggcat ctggctggtt caccagagag aaaggctggg
    19381 taaatttccc gtctttatat tgctgtccgc gatcggttgg tttggaagag caggcggcaa
    19441 gcattgccac aaccgtgccc ataagaaggt actttaccca acgtcctttc attgttctct
    19501 tcttcaggtt aaaaaataag gcgcaacgaa gataacaaac cgccgcgttt aatgaaatga
    19561 gcagcctctc cctgacgcaa attttgcaca aaaaataggc tttagtggtt tgtttttgtt
    19621 caaaatcatg ctaaatccgt gatcggggta aaaaaaaggt tgcatgaaaa cgcgagcgga
    19681 gtatagtgcg catccacgga cgcggggtgg agcagcctgg tagctcgtcg ggctcataac
    19741 ccgaaggtcg tcggttcaaa tccggccccc gcaaccaatc aaatttgatg aagtaaaagc
    19801 agtacggtga cgcggggtgg agcagcctgg tagctcgtcg ggctcataac ccgaaggtcg
    19861 tcggttcaaa tccggccccc gcaaccaatt attgaacacc ctaacgggtg tttttttgtt
    19921 tctgatctcc cataaaaaaa gcgccattca gcgccttttt atcatcccct tctcgccagc
    19981 gttgccccat cggcaaaata cgctttaatc cccgcaagaa tagactccgc aacttcctgc
    20041 tggaaagtcg ccgttttcag tttacgctct tcctcaacgt tactgataaa cgccgtttcg
    20101 accagaatgg agggaatatc tggtgccttt agtacggcaa acccggcctg ttcaacttga
    20161 tttttatgca gcttgttgat ttttccgagc ttattcagta ccgctttacc aaacttaagg
    20221 ctgtcagcaa tagtcagcga ctgcaccata tcgaacatgg tgtggtcgac atagcggtca
    20281 ccgcttttgc tcacgccacc aatcaagtcc gaggcgttct gggtttgtgc cagatatttt
    20341 gccgcagtac tggttgcgcc tttggttgag agcgcaaaca ccgaggaacc gctcggctga
    20401 cgactggtaa aggcgtcggc atggatagag acaaacaggt cagcacgctg tttctgggct
    20461 tttgctacgc gcacttgcaa cggaatgaag atgtcttcat tgcgcgtcat gtacaccttc
    20521 atattgccct cttcctcgat cagagagcgc aaacggcgag ctatttgcaa taccacatct
    20581 ttttcgcgcg ttttgtattt ccccaccgca ccggagtctt cgccaccgtg gccagggtca
    20641 agcataatga caatcggacg atcgcgccct gctttacccg gttgtggacc actttgcgct
    20701 ggcggcacct gcttttcgag gtcgcctttg ttgtaatcct ccagcagcgc cagcagcggg
    20761 tcctgcatat cctgtgcatt ggccggatag aggtccatca ccagacgctc tttaaacccg
    20821 gcgaccggcg caagggcaaa cagctgcggt tttacgtttt gctttaattc aaaaaccatg
    20881 cgtacggttt gcgggtcaaa ttgcccgacg cgcgccgact tgatgaacgg gtcgtctgca
    20941 cgaatttgtg ccgccatccc cttgagtacc gagttcaggt ttacatcttc gatatccacc
    21001 accacgcgtt cagggttact caacgcgaac tgcttatatt tcagctgacg atttgattct
    21061 accgtcacgc gggtgtagct ggacgcaggc cagacgcgca ccgccacgac ctggctaacc
    21121 gcagccaggc tgacctgact tacgctcaat agccacatag cgcccgcgcc ttgcagtaaa
    21181 cgacgacggc tgattgcagt gttggatcct gacatgcctc tcccgagcaa aagaaatcta
    21241 agctgtgtaa caagtaaacg actaatttga ccggaaactt tagcgaaaga cgcataatct
    21301 gtcatctaat aaacggtaaa cattcttttt atattcacgg cattactgat aaaaaagtcg
    21361 ctctcgcata aaatttacac ttgcaccctg cgaaaaaaca gaataaaaat acaataattt
    21421 caaataatca tgcaaagggg tgtgccgtgg taaaggaacg taaaaccgag ctggtcgagg
    21481 gattccgcca ttcggttccc tatatcaata cccaccgggg aaaaacgttt gtcatcatgc
    21541 tcggcggtga agccattgag catgagaatt tctccagtat cgttaatgat atcgggttgt
    21601 tgcacagcct cggcatccgt ctggtggtgg tctatggcgc acgtccgcag atcgacgcaa
    21661 atctggctgc gcatcaccac gaaccgctgt atcacaagaa tatacgtgtg accgacgcca
    21721 aaacactgga actggtgaag caggctgcgg gaacattgca actggatatt actgctcgcc
    21781 tgtcaatgag tctcaataac acgccgctgc agggcgcgca tatcaacgtc gttagtggca
    21841 attttattat tgcccagccg ctgggcgtcg atgacggcgt ggattactgc catagcgggc
    21901 gtatccggcg gattgatgaa gacgcgctcc atcgtcaact ggaaagcggt gcaatagtgc
    21961 taatggggcc ggtcgctgtt tcagtcactg gcgagagctt taatctgacc tcggaagaga
    22021 ttgccactca actagccatc aaactgaaag ctgaaaagat gattggtttt tgctcttccc
    22081 agggcgttac taatgacgac ggtgatattg tctccgaact tttccctaac gaagcgcaag
    22141 cacgggtaga agcccaggaa gagaaaggcg attacaactc cggtacggtg cgctttttgc
    22201 gtggcgcagt gaaagcctgc cgcagcggcg tgcgccgctg tcatttaatc agttatcagg
    22261 aagatggcgc gctgttgcaa gagttgttct cacgcgacgg tatcggtacg cagattgtga
    22321 tggaaagcgc cgagcagatt cgtcgcgcaa caatcaacga tattggcggt attctggagt
    22381 tgattcgccc gctggagcag caaggtattc tggtgcgccg ttctcgcgag cagctggaga
    22441 tggaaatcga caaattcacc attattcagc gcgataacac gactattgcc tgcgccgcgc
    22501 tctatccgtt cccggaagag aagattgggg aaatggcctg tgtggcagtt cacccggatt
    22561 accgcagttc atcacggggc gaggttctgc tggaacgcat tgccgctcag gcgaagcaga
    22621 gcggcttaag caaattgttt gtgctgacca cgcgcagtat tcactggttc caggaacgtg
    22681 gatttacccc ggtggatatt gatttactgc ccgagagcaa aaagcagttg tacaactacc
    22741 agcgtaaatc caaagtgttg atggcggatt tagggtaagc gatgaaaatc accggatgcg
    22801 gcaggcgtaa cactcgtgcg tcgcatccgg caattacgtt tattcccgcg aactaaacaa
    22861 cgccgccaga ccactgcgcc gctcagtacg agtggcgatt gccgcactta atatacgctc
    22921 atcggcatac agcgacagac gacgacgcgc gcgggtcacc gcggtgtata ccagctctcg
    22981 cgttactacc ggcgtgcgtt ggctcggtaa aatcaacgcc gcatggtcga actccgatcc
    23041 ctgcgattta tgtaccgtca tcgcccacgt agtttcgtgc tctggcaagc gactcggttg
    23101 cacagactta atattgccgt ccggcatcgc aaaccagacg cgcgtcccct gcccgcgatc
    23161 cagcgcaata ccgatatcgc cattaaacaa cccaagcgcg ctgtcattac gggcaatcat
    23221 caccggtcgg ccttcgtacc aacgagagtg cggatgacga tgaattttgc gcttctgttg
    23281 cataaactgt tcaattcgct cattcagtcc agccacgcca aacggccctt cccgcagggc
    23341 gcacaaaagc tggtactcat tgaacgcctg aatgattaaa tccggctcgg cacgcgcttg
    23401 cagcagatcc agataacgtc cgtaacccgc aagagcttcc tcaagcatcg caatataatc
    23461 ttcgccgctc tgtaaaagtc gtttttcgat atcagtaaaa tcctgctgaa aaacggtttt
    23521 cactgccgtt ttatcaccac ggttgatcgc cgcagctaac tgaccaatgc cagaatcgct
    23581 gccgaaacga tagctttttt gcagcaggca gagactgtcg cgcaaagatg ccgcttctgt
    23641 gccggttcct gccggaacgt gagtccccgt caggcggctt agctgcctgg cacgctcggc
    23701 ggtaaagccc gcgttggcat aagcgcagat atcgcccagc acagccccag cctcaaccga
    23761 ggccagttga tcacgatcgc cgagaaagat cactcgcgca tgatctggca aggcgtcgat
    23821 cagtctcgac atcataggca gatcgatcat tgacgcttca tctaccacca gcacatcaag
    23881 atgcagcggg ttaccggcat gatgacgtaa acgctggcta cccggctgcg cgcccagcaa
    23941 tcggtgcaaa gtgctggcat cttccggaat gcgtttcttt tgttcatcgg tcagcggtaa
    24001 ctgtcgcaaa gccttgccga gagattcggt taagcgcgcg gcagctttac ccgttggtgc
    24061 agccagacgg atacggcagc gttcgccgtc ggccatttga attaacgctg ccagcaactt
    24121 cgccacggtg gtcgttttac cggtgcctgg gccgccggaa atcaccgaga tccgccgcgt
    24181 cagcgccact gccgctgcaa ctttttgcca gttaatttca tcgcttactg gaaaaagttt
    24241 gtccagggtt tgcgccagta gagcttcatc aacctcaatg gtatgattca cctcattgaa
    24301 gaagcgcgct accgtccgct cgttacacca catgcgattc aaataaagac gatcgccaca
    24361 gaggatcatc ggcgtgggtt catctcccct gctgaccgct tgagaagcca gcaagcattc
    24421 ttcccaattt tgtagctcac cgatttcact gacacaggtc gccaacagcg gattcgacgc
    24481 ctcgttattt tccagtcgtg aaagcggcaa acagacgtgt ccctctccgg catcatgact
    24541 taacagtgcc gccgcgaggg tgacggcagg atgttcatct cccgccacgg tcagggcaaa
    24601 ttgcacatcc agcgggcgta gctgtttgtg ctccacagct tccagtaatt gcttttgcaa
    24661 tttcattacg cctcctccag ggtcataccg gcaaacatct catccatcag ggcaatcaac
    24721 ccggcgttgg gtcgggttgt gtaaatccct tgttgcggat gttctttatc aacgccacgc
    24781 aggaacagat agataacgcc gccaaagtga agatcatagt cgtaatcagc aatgcgatgg
    24841 cgcagataac gatgcagcgc cagggtataa agctgatatt gcagatcata gcggtgtgcc
    24901 tgcatcgctg ctgccatagc ctgttgggtg taagccgaac tgtcttcacc cagccagttg
    24961 gatttatagt cgagcaggta ataacgccct tcgtggcgga ataccaggtc gataaagcct
    25021 tttaacatgc cgcgtacctg catgaactcc agcggcgggc agccagcgga tagcgggtca
    25081 aactggcgaa ttagtgcatc aagctggctg gcgatgagcg gttcgctaat cggcaggtaa
    25141 aactccatct ccacctgttt atcgcggtcg gaaagctgac tcaggctaac acccgtttca
    25201 ttgagaggtg cctggaggac agccgtgatc cactcggtca acaccggttc ccactgcgat
    25261 tcaaagccgc cgagttccag tttttcctgt acccagttcg ggtcaaccgg ctgggtaaaa
    25321 tcgaggtctt caaacaaact gtgcaagaat gtccccggtg acgcaccgcg cgggaactga
    25381 tgcggtgtta acgtcggttc ttcaacgacg ctgaccacgc ctgcggcatc aacatccagc
    25441 cgtggcatca gatcctgagc gataccgtga ccacgctgct gcaagccgga gtagctggtg
    25501 acacgccagt tatcgccggg caatcgttgt agtgtcctcg cattcagttc tgcagttaaa
    25561 gcatcattaa cctgccaggg ctggttatca ccagtttgtg ccgtttgcca ggcaatatca
    25621 tcatcgcata acgcttcaat acaggtgcga agccctgccg catcttgcgg ttcccctttt
    25681 tgcagcaaac gcccgagcgc actttggtgg acgtcggtgt cacctttttt atcgccacga
    25741 cggcgcacca gcggtgcaac gccgagactg caatgccaaa ccgaacgcgt cagcgccacg
    25801 taaagcaaac gcagatcttc cgccagacgt tcgacctccg cgaggtcgac gctttctggc
    25861 gcagcattaa gatccaaaac tgcctcaaac gagtggcgat cgtgataaaa cgcctgatcc
    25921 tggacgcgga aattggtaat aaatggcaac cagaccagcg gatattccag ccctttcgat
    25981 ttatggatcg tgacaatctg caccagatgt ttatcacttt cgaggcgcat ttgttggctg
    26041 gaggcattac tgtctggctc gaggatatgt tgcgataacc agcgtaccag cgcatgttca
    26101 ctttccagct gcgttccggc ttcttgaagc agttcgctga tatgcaagat atcggtaaga
    26161 cgccgctcac cgcctgccgt tgccagcaag ttttctgcaa tgttacgcgc cgacatcagc
    26221 gcccgcagca tcggcataac gccacgtttg cgccagattt gccgataacc atcgaactct
    26281 tcgactaccg catcccacgc atgttcgtca ttattcagcg tttcaatatc cagcgcattc
    26341 agccccatca ttgacgttgc cagcgcactg cgcagggtgt tctcacgttc gggcgtcatc
    26401 accgcctgca acaaccaaag catttcctgc gcttccagag tttcaaaaac actgtcgcgg
    26461 ttcgaaaggt aaacggaagg gatttccagc aacgttaagg catcgcgcac ctgggcggcc
    26521 tcctggcggc tgcgcaccag cacgctgatg tccgaagcac gcaccggacg cgcgtcgtcg
    26581 ccgttcatca gcaacgcttc gccccgctgt ccggcttgta gccagtcgcg gatttgcgca
    26641 gcacatacct gcgccatggt actttgatag tcgccaacgc cgcagctttc gccttccatc
    26701 agccacattt tcatcgcagg ctgcgtttca cctttaaata caaaacgtaa cgcctgattt
    26761 ttcccggctg atttcactgg aataaacggt atttcgcgaa acatgaacgt gtcatcagtc
    26821 tggctgaaaa gcttgttcac actgttcacc attccaggtg cggagcgcca gttggtgtct
    26881 aaagtgtagt gggcatgaac ttcactacgc gccttcatat aagtgaagat atccgcaccc
    26941 cggaatgcat atatggcctg cttcgggtcg ccaatcagca acaatgcggt ttccggctga
    27001 tggtgccaga tacggcgaaa aattcggtac tgctgggggt cggtatcctg aaattcatcg
    27061 atcattgcca ccgggaatcg cgtacggatc gccgctgcca acacctcgcc gctttcgcta
    27121 cgcagcgcgg aatcgagccg acttaacatg tcatcaaaac ccaattcgcc acggcggcgt
    27181 ttttcacgcg ctactgtttc gcggatctca gccaatgcgc gggtgatcaa cagatcgcgg
    27241 atcgacaatg gttctgcaag cagttgatcg atcgcctcaa acagtggatg tcgcggggtt
    27301 tcccccccgg ccttcgtgcg atcttctaag aaacgctggg agaatttttc cagcgactcc
    27361 ggcaactgat aactgtttgt ctcttcttct gcccaggcgc tgatcttttc gatccattta
    27421 gcctgattgc tacggttaaa cttgcgtcga tcaataccag aagattcgat cagcgcatcc
    27481 agttcaccca ctgcgtcgcg ccactgctgt tttaccgtat caatacgcgc cacaatttgc
    27541 gcgtggcggg aagccagcgt ttcatcatcg ggcggcggtg ctttgataac cggcggttcg
    27601 ccttgcagat aacgattaat atcgcgcagc aacgcctgcg gccctttcca ggtttcaaag
    27661 acgacctggg ctatttcacg cggcagcggg tagcagtggc gacgccagaa atcggcgcag
    27721 gcctggtagc gtagcagaga ctcatcttca atcagctgct gctcaaacag catgccggat
    27781 tcaaaggcat tcaggttgag catgcgctgg caaaagccgt gaatagtaaa gactgccgct
    27841 tcatccatct gccgttcggc taacaacaac cactgcgcgg cttgcgcttt atcgtcgatc
    27901 tcttccagca ggcgttcgta cagtggattg tcggtggttt cacgcagaca ggcgatgcgc
    27961 aactcgtgga tattgctacg gatacgaccg cgcaattctg ccgtggcagc ctcggtaaag
    28021 gtgaccacca gcagttcttc aacgatcagc gggcggggaa aggcggcgga accgcctagt
    28081 ccaagtaaca ggcgcaaata gagcgccgca atcgtaaagg ttttgcctgt gccggcagag
    28141 gcttcaatca ggcgctcgcc ctgtaagggc aagcgcaaag gatctagtgt ctcggcgaca
    28201 tcactcattc ttttcactca tcaggggcat tgtttgctgc aacgcgctga cgttctccca
    28261 cactttccag ccttcaggat gcacatattc ggctttcccg ttctggctgc cggaaatctg
    28321 cgacagaata gccatgcctt gcggctcgac caccgcctga tggaagaaat cagcaagttt
    28381 ttgcggcgtc agcagtttta tctgggccac gattttatca cgcgaatcga agcgcatatt
    28441 gccgcgatcg aaatctttac ttaacttcga tgcttcttcg ccgagcgttt gcggtgcctg
    28501 cagcatctgg gtaattaccg cctgctggat ttgcgcaaac tcatctggct tcatcgctcg
    28561 caattttgcc tctgcggttg ggaaaaacgc cttgtaacgc tcccacaaga atgaaggctg
    28621 tttatcattg ctttgcaaaa ggaagcccat gccccactga cgccccacgc tcattggaaa
    28681 cgcaaacacg gcatagccca attgttcttc ggtacgcaac tgattgtaga accacggctg
    28741 tacgatctgc cccaacagag agctataggc tgagctggtg tattcatcgt agccagtcgg
    28801 tacaaatacc gctgccagtg cggagtcggt gctgttaccg gctttttcaa agatgacgga
    28861 ttgtttttta tcgaccacta catctttgtt tcgacaccac tctgaaccat cagcgcccaa
    28921 ctgtttttgc acatcgcgtg ccagcgttgt tgcctgggcc tcggtcatgt tgccgataac
    28981 cataaactct ggtcgagccc ctgattttaa ggcgtcgcga taggccagca cctctttcaa
    29041 cgtaatggag ggcaaaattt tacgccgttc atctcgcgag aagtacggca cttgcgagag
    29101 catctgcgcg ggcataatcg cctgctcaaa cgctttaccc ttttctgcgg aatccatcat
    29161 ctggttatac caggacttcg cctgctcaag ctgctcttcc gtagcggtat agctaaagta
    29221 cccctcgagc aatgcctgga acagctgcgg cagacgctgg gtgtaaccat tagcattaat
    29281 cataaggccg ttgttagcgt tggtggaaaa acttatgcca ccaaccgacg cctggttgct
    29341 taactgatca agcgccagcc ctgcgagata atcattgagc gcaaacatca cctgattgcg
    29401 ggcgctgtcc atggctttcg gattacgcaa aatcaggctg acatcagctt tgggctcgct
    29461 ggcaaaataa cggcttggcg catacaccac gcgcagattc gactcatcaa caatcagctc
    29521 tggatggtcg tatttcttct ctgacttaat cagcgagaaa tcatcaggaa tataagggtt
    29581 aagctctggc aaagagagcg caatgtcggc ggcttttttc tgccagtcgg cgaaagtttg
    29641 tgcgctgatt ttatcgacct gatacggcgc atcgacaaag taagccgttt tgttgtgcgg
    29701 ctctttcggg ctgatatacc agatacgcgc attctgcggc gtcatcatcg ccagacgttc
    29761 ctttactgct ttagcatcgt accgatcggc aatattgact gcatccagcg tatgctcaac
    29821 aggaacgcga atcatggtat ctgccagcca ttcgacgtaa tccatatcac gggtgatcga
    29881 cggataacgg aagtcgatat ccagcacatt cgccagttca tcgaagtatt gtttatcaat
    29941 gcctttttca cgtaacagat tgagatagct aaaaattgcc gccacaacct gatcgcgatt
    30001 agccaggcct ttatcagtta aagacgcaga gatcgctaat acgccgctgt tgccgttgac
    30061 gataggatcg gagttggcgc taatgccctc aactaatccc tgcttttgca gccagtcaga
    30121 aagtgtacct gggctgcgat tgccaatcag ataggtaatc aattcatcgg ttttactacg
    30181 gaacttcgct gagttgttat cgatgcgaaa ctcaacgcgc aacactttac gcggcagcgc
    30241 agggacgtaa tgaatgataa tgcccttttg cgcgtcggtg actaccggca cggtgatttc
    30301 cggttttttg ctctctttgt tcggcacgcg accaaaggtg tccgccgcca tttttgccaa
    30361 ctccggcagc ggtttattac tgtaaataac cgccttcatc aaattggcgg aatagtactt
    30421 ctcgtggaaa tctttcagcg cctgctgcac cggattacca ggtttgtcgc ttaaagtttc
    30481 gaggttacca ccagaaaact ttgaaccggg gtgtgccggg ttaatggttt ctgcgctgac
    30541 ctgtgccatg cgcatcccgt cacgcgtacg cgccatggtt aattcagcgt tcactgcatt
    30601 acgctcacgt tcggcgtatt tcttgtcgag caagggttct gcaatagcat cggccaggcg
    30661 gtctaccgca ccgggcaagg cgtcgttctc aacttccaga tagaaagccg tgcgatacgg
    30721 cgcagtgctg gcattgtgac taccgccgtg cattttgaga tattcggcca gactgtcagc
    30781 ctgcgggtac tttttcgacc ccatcagact catatgttca aggtaatgtg ccagcccctg
    30841 gtacgcctcg ggatcttcca gcgacccaac gggcactacc agcgccgaga gcgatttaac
    30901 tgcctgcgga tcagaaacca gcaagaccac cataccgtta tccagacgta tagcctgata
    30961 ctggcggtta tctttatcac ttttacggat ggtttcctga atcggctgcc atcccgtttc
    31021 tgcctgactt aatggtgccc agagggcaac aaacaacaat aatgctttga accaggtgct
    31081 gcggggcatt cacggacctc ataatcaact ttattttctg gacagattca acaggttaac
    31141 gcaaatcatc tgccagaatt taatcttgtg ctgcacgagt cagcctatgt ttatataacc
    31201 atcagtccgt gactggtgcg catcataaag taagcagata gattgcgcaa tttttataca
    31261 acactcatga ctgattaaag cgaaacagcg gtaacaggaa acgttgcgac tgttcaacga
    31321 tggcctccat tgtctctggt gttaattgcc gccagagccg ttgataccag atatcatcac
    31381 cttcgccacg caccatcatg ttgccttcgt aagcctgaag gaatttcgta cgggcttttt
    31441 gcaacgtgga atcgtcatcc agcatggcat cgttttgcgc gtcataacag gtttttagcc
    31501 acgcgccgcc actttcaggt aacaccagca atggcgcgga cattccttca cgatacccct
    31561 caatcagttg tgagaggtaa tgcaaagcct gttcggctgc aagcggcgga aaacgccact
    31621 caccgtcttt gcgtagaaaa aggcgacttt cgccattacc accgctggca cagtagacaa
    31681 ggtgttccag ccaaagttgc attccctgcg ccacacttaa taaagaggga cgccagcgca
    31741 acaggccatc cggctgcacc tgcggcaacc agccagttat ctgcacaccg ttgcaggcga
    31801 gatcaatttc catactctgc cccggctggc gacaggcaat gactctgtcg gcaagctgct
    31861 gcatctcctg gcactgtgtt tcccagaaaa tttcaccaaa agcgccatac ggtaaatccc
    31921 ctgccgcccg gaagcggcgg aacaagcgtt cggcatcatc ctgctcaacc agtgcattca
    31981 ataactgctg attgatttga taacggctaa gtccttccag aataaatggc tcggtgtctg
    32041 ggatttcgct gtcttcagta tggaagttca cctgcaaacg catctggaag aacgcccgca
    32101 ccggatgtgc ccagaatcgt tgtagcgttt ccagcggcac ggtttccggt aaggtaaacg
    32161 gcagcggctg aacaaattca gaatgtgctt taccagcctg gctggccgca ggtagccatt
    32221 cacgagcata gctttgtcgt tcgcctggct ggtagttttg cggatcaaac ggcatccggg
    32281 tatggaggca agtaagatgc gcttttaccc ttgcctcgct ttcatcacag ttgagcgctt
    32341 catcgcccgg tagataatga ctttgcccga tgtagtcgat cagttcctgc accagtaccg
    32401 acgggaaacg ttcactgtta tcctgaatgg aacgcccgat atagctgata tagagttttt
    32461 gctgcgcgga aattaacgct tccaggaaca ggtagcggtc gtcatcgcga cggctacggt
    32521 cgccacgctt cggtttctgg ctcatcaggt caaagcccaa tggcgcaagc tgacgtggat
    32581 aaacgccgtc gttcattccc agcaggcaaa ccactttgaa cggaattgaa cgcattggca
    32641 tcagagtaca aatgttaacc ggtccggcga gaaaacgctg gctgatacgt tcttgatcca
    32701 gacgctgtgc cagttcatca cgcaatagtg acagcggcac cgcgtcgcca tactgcgcac
    32761 ctaaaccttc ggcgataatc gcctgccatt gttgttcgat cagcgtcatc gccgcttcgg
    32821 tttccgcatc cggcaggaag aaggcgttga gcatatcgcg acaaaccggc aaccactctt
    32881 ctagcggacg ctcctgtgcc agcccgcgac gccagatgtt tagctgcatt agcagtgaag
    32941 ccagatgccc caccagttct gcaattaagc cgctcgattc atcataaggt agaaccgatt
    33001 gccactcgcc ctgcgcgctc tccatcgcgt agcccagcaa catacgcgtc aggccaaatc
    33061 gccaggtgtg ttgtccggtg gcggggagtt ccagctcgcg aacgttgtcg tcatctatcc
    33121 cccaacgaat gccggattcg ttgacccact ggcgtaaata acgcagccct tcttcggtga
    33181 tgtcaaaccg cgccgccagc accggcacat ccagcagcgc cagcacatcc tctgacacaa
    33241 agcgactgtc aggcagtgat aacaggctga taaacgcttc cagtaccgga tgtgactgcc
    33301 gcgcacgacg gtcggaaatg gcgtaaggca ggtaacgatc cgcaggtgcg ctaccaaaca
    33361 ctgcctgaat aaacggactg tagctgtcga tatcagccac catcacgatg atgtcgcgcg
    33421 gagtaagtgt cggggcttcc tccagcatcg ccagcaagcg atcgtgtaaa acttcaactt
    33481 cacgctgcgg gctatggcaa acgtggaatg tgatactgct atccagtgga tcaagcgggc
    33541 gtttgttatc gctacgggaa aactcttcga tgttcacacc cgcaacggcg cggttttcca
    33601 gttccagaat gtcagactga atgttatgca gcaggttatc tggcgtcaca tcgacaaaag
    33661 cgtccagctc ctggctgctc tccaggtcag aaaggagata aatgtagtcg cgcccaagct
    33721 tcccccatga ggccagcagc gagttgccga catcctgttc accatcgctg ttaaagagct
    33781 gtccggcatt ttcgctatcg cgaaatagcg gcaattcgcg atcttcaaaa ctgtgtcgac
    33841 gctgacgagt cagcagtttc gccagataag caggatcttt aatatcgccc cagtaataac
    33901 ggcaggggtt ggtaaacagg agatggattt caatatgttt acccagcgcc tggagcgcct
    33961 ggagataaac aggcggtaac gcggaaatac cgcatataaa gacgcgcgaa ggtaaccccg
    34021 gcgggcaggt cgtcgcggac tccagcgttt cgataaagcg ctgatagaga ttcgcgcggt
    34081 gccagcgcgg ttgtccgagt tcatgggtat attccaccag cgccttccac aacggggctt
    34141 gccaggcctg tgcttctccc agcccttcaa ccaaatgtcc tgtttcccac tgtgccagcc
    34201 agtccggacg atagaccaga tactggtcaa acaggtccgc cgcttttgag gaaagctgga
    34261 acagttttcg cttgtcgcta tcgtcagtca gataatgccg caacagggta aagtcttcgc
    34321 gctccagcaa ttgcggcagc agagtcatca gtttccagct catgctctgt ttgttaaagg
    34381 cgctctcttt ggggatttcc ggtaacaccc ggacgaacat atcccagata aagctcgctg
    34441 gcagcggaaa atcaatgttt gccgcaatac caaacttttg cgacagggtc atttgcagcc
    34501 actgcgccat accggtactt tgcaccagaa tcatctctgg ttcgaaagga tcgtccagcc
    34561 gttcgcgttc gacaataaac tccatcaacg cttccagcac gtccagacga ttggaatggt
    34621 agacccttaa catagcggct cctgactact gacaattcgg gcaatgcagg cgggtcatct
    34681 cgccttctct gcccccgggt gaaactagcg taacgctgat gctgacacat cccgcctgcg
    34741 atgtctgcat tcggttgacc tgccagttgg caggtggcga aatcgcgcgc agttgcgttt
    34801 gctgccaacc atgccgccag agcttttggt actggtttct gctggcgaga ctgttcattg
    34861 atgttcgctg gatacccgat aacgcagtga caatcatcac catcagcacc atcagcacca
    34921 tcagcaccat cagcaccatc gcaaccatta cctccggcag gctaaagcct tgttgattct
    34981 tcagggaagc tgacataatg ccccctcttt cagcggacaa aaatcgctcc agccgcgtgg
    35041 cgaaaaaaca atttttccat caatgacttc gcctgttcgc cacaacgaaa cgccttcata
    35101 accggcaatc aataaggctt cattatctgc cagtaaacgc aaacaaacct gggcatcggt
    35161 tccagcgtac tgcgagcact gaactgctgg ctgcgtctgc caggagtgca tttttcccca
    35221 cgccagcgcc gactgaacga tggcctggcg gcgcaatgac tgactttcca tgctcacgcg
    35281 agaggcaaaa ctgcgatcct gctgactcat tccttgcaat agcaagctac ccaaaaccag
    35341 cagcatcagg accagagcca gtgacgaaac acctttttcg cggttcacag gttgaatcct
    35401 gtcacgctat agctggcatc caccacggtt tgcggttcag acttactggc agcacgcata
    35461 ttaaccgtca acaccggcga gaagccgctg acatcctgac gtacgacctg aaaagtgtcg
    35521 ataatgatgg catccggatt agtgacttta tcccagccct taccttcaca ggatgtcgca
    35581 ccgcgtagcg tttccagcac atgctccttc agacgaaatc caatctggtc ggactctttt
    35641 accggttcgc gatcccagat accgttactg ttcgcatccc actgcacaat gatacagtca
    35701 ccctgtccga caatttccag cccttcgccg gtacagctgc catgacaata acccgccctc
    35761 tggagatgct tcgcgacggt aaatacccgc agccagattt catcttccag tgccagctta
    35821 cgagtgctcg ttaaactttc acgctgtaac gcaggcagaa agcgtgcagc ccccagcaac
    35881 aatacgctac tgatcgccat agcaatcaac acttccagca gagaaaaacc ttgctctttt
    35941 acaggcatcc ttctgtttct ccttgctgat aaagccggag tcttccccac ggcgaaacca
    36001 ccagccacca ctcgcccgtt gagtttttga agcgaatatg cacggcccat gcggtattgc
    36061 gcaggccaaa gaaagcaagc gaaggtgtca ggtcgctcat ttcgacttcg ggccagcgtg
    36121 gcacaaagac caatggtgaa ctgccatgac aggtattggc cccagcagcg gaactcacaa
    36181 ggcaccataa cgtcccctcc ctgataacgc tgatactgtg gtcgcggtta tgccagttgg
    36241 catcttcacg taaatagagc aaatagtccc gcgcctagct ggcggtttgc caaagccgtt
    36301 gcgactgctg ccagtattgc cagccataga gtccacttgc gcttagcatg accaaaatca
    36361 gcatcgcgac cagcgtttca atcagcgtat aaccacgttg tgttttcatg ccggcagtat
    36421 ggagcgagga gaaaaaaaga cgagggccag tttctatttc ttcggcgcat cttccggact
    36481 atttacgccg ttgcaggacg ttgcaaaatt tcgggaaggc gtctcgaaga atttaacgga
    36541 gggtaaaaaa accgacgcac actggcgtcg gctctggcag gatgtttcgt aattagatag
    36601 ccaccggcgc tttaacgccc ggatgtggat cgtagccttc aatctcaaag tcttcgaaac
    36661 ggtagtcgaa gatggattcg ggtttacgtt tgataatcaa cttcggcagc ggacgcggtt
    36721 cgcggcttaa ttgcagatga gtttgatcca tatggttgct gtacaggtgc gtatcgccac
    36781 cggtccagac aaaatcaccc acttccagat cgcactgctg cgccatcata tgcaccagta
    36841 acgcgtagct ggcaatgttg aacggcaggc cgaggaagac gtcacaggag cgctgataaa
    36901 gctggcaaga gagtttgccg tctgccacat agaactggaa gaatgcatgg cacggtgcca
    36961 gcgccatttt atccagttcg cctacgttcc acgctgaaac aataatgcgg cgcgaatccg
    37021 ggtcgttttt cagctggttc agtaccgtag taatctggtc aatatgacga ccatctggcg
    37081 ttggccaggc gcgccactgt ttaccataca ctggcccgag gtcgccattt tcatcggccc
    37141 attcgtccca gatggtgaca ttgttttcgt gtagataagc aatgttagta tcgccctgca
    37201 ggaaccacag cagttcgtgg atgatggaac gcaggtggca acgtttggtt gtcaccagcg
    37261 ggaatccatc ctgcaggtta aaacgcatct gatgaccaaa aatggaaagc gttccggttc
    37321 cggtacggtc gtttttctgt gtgccttcgt cgagcacttt ttgcatcagt tctaaatact
    37381 gtttcatggt tcctcaggaa acgtgttgct gtgggctgcg acgatatgcc cagaccatca
    37441 tgatcacacc cgcgacaatc atcgggatgg aaagaatttg ccccatgctg atgtactgca
    37501 cccaggcacc ggtaaactgc gcgtcgggct ggcggaaaaa ctcaacaatg atgcgaaacg
    37561 cgccgtagcc aatcaggaac aagcctgaga cagctcccat tgggcgcggt ttacgaatat
    37621 acaggttgag gataataaac agcaccacac cttccagcag cagttcgtaa agctgtgatg
    37681 ggtggcgcgg cagcacaccg taagtgtcga aaatggattg ccactgcggg ttggtttgca
    37741 gcagcaaaat atcttctgta cgggagcctg ggaacagcat ggcaaacggg aagttcgggt
    37801 caacgcggcc ccacaattca ccgttaataa agttgcccag acgcccggca ccaagaccaa
    37861 acggaatgag tggtgcgata aaatcagaga cctggaagaa ggaacgttta gtacggcggg
    37921 cgaagataat catcaccacg ataacgccaa tcaggccgcc gtggaaagac atgccgccgt
    37981 cccagacacg gaacagatac agcggatcgg ccataaactg cgggaaattg tagaacagaa
    38041 cataaccaat acgtcccccg aggaagacgc cgaggaagcc cgcatagagt aagttttcaa
    38101 cttcattttt ggtccagcca ctgcccggac gattcgcccg acgtgttgcc agccacattg
    38161 caaaaatgaa ccccaccaga tacatcaggc cgtaccagtg aagcgccacg ggtcctattg
    38221 agaaaatgac cggatcaaac tccggaaaat gcagatagct actggtcatc tgtcaccaca
    38281 agttcttgtt atttcgctga aagagaacag cgattgaaat gcgcgccgca ggtttcaggc
    38341 gctccaaagg tgcgaataat agcacaaggg gacctggctg gttgccggat accgttaaaa
    38401 gatatgtata tgatccgcgc tataaccctc cgcgaatcag cccgcccatg ccgcgacgct
    38461 ccataaaggc tgcaacctga tgacgaactt cggtcgccag ttgcgcttcc agactacgct
    38521 gcgcaagatt ttctgcttcg gcaaaatcaa tgcgccgcag caggtatttt acccgcgcta
    38581 cagaacgtcc gttcatagac agatggcgat acccaagccc aatgaggatt gccacgcaca
    38641 tgggatcgcc cgccatttca ccgcacaaac gaagatcgat accatgtatt tccgcttccc
    38701 gggcgatcat cgccagagct cgtaacattg caggatgaag actgtcgtaa atgttcgcca
    38761 cccgggtatt gttgcgatca acggccagaa tgtattgagt cagatcgttg gtgccaacag
    38821 agatgaaatc gaccctcttt gccagatgcg gcagcataaa taccattgac ggcacttcca
    38881 gcatgatgcc gatacgtggt ttgggaattt cgtaaccgat catctcctcg acttcacgtc
    38941 cggcacgttc aatcaggcgg cgtgcttcgt caacttcatc gaggctggtg accatcggca
    39001 acagaatatt caggttgccc gtagcggcat tagcacgcag catcgcccgc acctggatca
    39061 agaagatctc cggctgatcg agcgtaatgc gaatcccacg ccaacccagg catggattct
    39121 cttcgctgat cggcatgtaa ggcagctgct tatctgctcc gacatccagc gtacgcaagg
    39181 tgacgggttt atcattgaac atttgcagca tcccctgata ctgcgccacc tgttcttctt
    39241 ccgacgggaa accactttgc agcatgaatg ggatttcagt gcgataaaga ccgatgccat
    39301 caatacagct gcccagtttt tcttcatgtt ccgggcttaa accagcattg agcatgactt
    39361 ttatacgctc accgcttttt aactgcgcgg gtaaattgac gtcatcttcc gccagacggc
    39421 taagctcaat ctcttcacta attagccgct gatattcttg cagcagtacc ggctccggat
    39481 cgaccagcaa ttcaccgcga tagccatcaa cgatcagcgt cctacgatgc agcaccgaag
    39541 gctgaatatc cgcgcccatc acggtaggga tccccagcgc acgtaccatg atcgcagcat
    39601 gggagttggc tgcgccatct cgcacgacaa caccgactaa gcgatcctgg ggcagctcag
    39661 caagagttgt cgctgacagt tcatctgcca ccagaatgaa acgttccggc caggcgttcg
    39721 gcccttgatt agcgtcatca agatgaaaca gcaatcgctg acccagcgca cgtaaatcac
    39781 cagcccgctc tttgagatag ttatcgctta gcgcggcaaa ctgttcggca aatttttcaa
    39841 tgaccgtttt taccgcccac tctgccaccg agcctttatc aacctcggca aacaattcgc
    39901 gacgcagccg ggtgtccgaa agcaggtgcg agtaaagatc gaaaatagcc gccgtttctt
    39961 tttgtgctcc ggcggcaaag cgtttgctgt agcggcgaaa ctcgtttgcc gcttcttcca
    40021 gcgccccggt cagtcgttcg cgttccagag ccggatccag cgttgatgcc tgatacacct
    40081 gttccattaa aggtaacgtg gcatcctgcc agccttcggc aatcgccaca ccaggtgctg
    40141 ccggtaatgc gcggattcgc gtctggcgat attgcccaaa cagggcagtc aactgcgact
    40201 gagaaagaat agctgccatc tgggtggcaa gcgtcaccag gaaggattct tcactttcgt
    40261 catactggcg caactctcgt tgctgtacca ccagtacacc aagcaactgg cgacgttgaa
    40321 taattggtac gcctaaaaac gcgcggaaac gttcttcttt tacggagggg atgtatttga
    40381 agctggggtg cttttgcgca tctgcaaggt ttatcggttc cgccagcctg ccaaccaggc
    40441 cgacgatccc ttcatcaaac gcgagcgtta cagtgcgacc gcgtggtttt ttcagccccc
    40501 gggtcgccat caggtagtaa caacgtcgat catgatcggc caggtagacc gaacagacct
    40561 cggtatccat cgcaagacag atgtcggtaa ccagaatatt taacgcctca ttcaggcgtg
    40621 gtgcgctggc taccttttcg actatttcgc gcaggcgagt gagcataatt ggcgtgactt
    40681 aacctctttt acgtcgataa gcagatgcgt tttgtggttt tggcgtattt tcctgcagtg
    40741 acatcaccac actcgcgaac tctttcatta ccctacggta gacatcacgt ttaaatgaca
    40801 ccacctgtct gaccggatac cagtaactta cccatcgcca gccgtcaaac tctggtgtac
    40861 tgctggtttg catattgatt tctgcatcgc cgcttaccag ctgcaagaga aaccattttt
    40921 gtttttggcc gatacaaacc ggcttcgtgt cccaacgcac caaacgtttc ggtaatttgt
    40981 agcgcaacca gttacgcgtt gaagcaagga ttcgaacgtc tttgcggctt aatcctactt
    41041 cttcaaacaa ttcacggtac atcgcctgct ctgcggattc tccggggttg attccgcctt
    41101 gcggaaattg ccaggagtgc tgaccaaatc gccgggccca cattacctgc ccctggcgat
    41161 tacaaatcac gatacctacg tttgggcggt agccatcgtc atcaatcacc ggactacctc
    41221 aaaataaagc tttatatacg aatgattgtt tcatactcca ggaagacggt aaaccactct
    41281 ctgcagggca ttacacacta ataacaattg aataactcac agttatgtgc agagttataa
    41341 acagaggaag gggtggatag ccgtttttat tcaccttttc tgtggataga gttgtgaaga
    41401 actacggaat tactacggga aaacccggag cattctgaat aagcccgata tagacatttt
    41461 aaaatattca ttattagtca atgcattaca tgtaatttga gtaatgaaaa agagtaattc
    41521 gtgacccagg tcacacctct catttacggg ttggcgaaag atcaaccaat gccgtgttta
    41581 tccacagaat gtgccactaa gttaagcact gaaccactaa aaactggagt ttcgtcgcac
    41641 gtcaaggctg taaatgaaaa cagtagtgga ggtttttcac agttatccca gctttctgtg
    41701 gataacatgg tgtaagatcc tgtttatttt cagtgaccag atttggaaaa cctattgcag
    41761 tgttgcgcaa ctcgattacc ggcagcctaa aaagcaatat aaatcagaga attgaacaac
    41821 acatgtggaa aaagttacac tgcgaatatt cggcacataa ttgctgtttg ttttttaacc
    41881 aaggtatcat gacatgtccc aacctcgccc actgctctct cctcccgaaa ctgaagaaca
    41941 gttgttagcg caagcacagc aactttctgg ttatacattg ggagaactgg cggcacttga
    42001 cgggctggtt acgccagaga atttaaaacg cgataagggc tggattggcg tgttactgga
    42061 gatctggcta ggtgccagcg cagggagtaa acctgagcaa gattttgctg ctctgggcgt
    42121 ggaacttaaa actatccctg tggatagtct tggtcgtccg ctggaaacaa cattcgtttg
    42181 tgttgcccgg ttaacgggta atagcggggt gacctgggaa accagccatg tgcgccacaa
    42241 gctcaaacgc gtactgtgga taccggttga aggcgagcgc agcatcccgc tggcgcagcg
    42301 tcgcgtagga tcaccgttgc tgtggagccc gaatgaagag gaagaccgac agctacgcga
    42361 agactgggaa gaattaatgg atatgattgt tctcggtcag gttgagcgga tcaccgctcg
    42421 tcacggggaa tatttacaga taagaccgaa agcagcgaat gcgaaagcgc ttaccgaagc
    42481 cattggtgcc cggggcgaac ggattctgac gctgccacgc ggcttttatt tgaagaagaa
    42541 tttcaccagt gcactactgg cccgtcattt tctgatccag tagccatcgc tttgacctgc
    42601 cgctttccgg gcatataatt accgcttcat ttttttggca gggcttttta gatgttattt
    42661 gcatggataa ccgatcctaa cgcttggctt gcgctcggta cgctgacgct gctggagatc
    42721 gttcttggga tcgacaatat tattttcctt tctctggtgg tggcaaagct tcccacagca
    42781 caacgtgctc atgcgcgccg tctggggttg gcgggagcca tggttatgcg tctggcgctg
    42841 ctggcatcaa tctcttgggt tacgcgcctg acaaatccgc tttttacaat attcagtcag
    42901 gaaatttctg cccgtgattt gattctgctt ctgggtggct tgttccttat ctggaaagcc
    42961 agcaaggaaa tccacgaatc cattgaaggt gaagaagaag ggctgaaaac acgcgtttca
    43021 tcattcctcg gcgctatcgt gcagattatg ctgctggata ttatcttcag cctcgactcg
    43081 gtgattaccg ctgtgggtct gtcagatcac ctgtttatta tgatggcagc cgtggtgatt
    43141 gccgtaggcg tgatgatgtt cgccgcgcgc tcgattggtg attttgtcga acgccatcct
    43201 tcggtaaaaa tgctggcgct ctctttcctg attctggtgg gctttaccct gattctggaa
    43261 agtttcgaca tccacgtacc gaaaggttac atctacttcg cgatgttctt ctctattgcg
    43321 gttgaaagcc tcaacttgat tcgcaacaaa aagaatccgc tctgatactc cgtacgctct
    43381 cctgcgggag ggcgtactca ctccttttac cctcacagtt taagattttc ctgctttcaa
    43441 gatatataac gtcggtttat aaacagacta ttatcatagg taggccaaat gaggataacc
    43501 ggatgaaaaa atgggcagta ataatttctg cagtcggact ggcgtttgct gtttccgggt
    43561 gttccagtga ttacgtcatg gcgaccaaag atggccgtat gattttgacc gatggaaaac
    43621 ctgaaattga tgatgatacc gggctggtga gttatcacga tcagcaaggt aacgcgatgc
    43681 aaattaaccg tgatgatgtt tcgcaaatta ttgaacgtta acaaataagg tcagcatccg
    43741 gctggcctta agatttttct ctttcccttt tcccttccct ctgccatttt tatattcctt
    43801 atgtcgtgat tataaaaagg aaacggctat gcaatatcac cgtatacccc acagttcgct
    43861 ggaagtcagc acgctggggc ttggcacgat gacgtttggt gaacagaaca gcgaagccga
    43921 cgcccacgca caactcgact atgccgtcgc tcagggcatt aaccttatcg acgttgccga
    43981 aatgtaccca gtacctccgc gccccgaaac gcaagggtta accgaaacct acgtcggcaa
    44041 ctggctggcg aaacatggca gccgcgaaaa gttaattatc gcctccaaag tgagcggacc
    44101 gtcgcgcaat aatgacaagg gcatccgccc ggatcaggcg ctggatcgga agaatatccg
    44161 cgaagcgctg catgacagcc tcaagcgcct acagactgat tacctcgatc tttatcaggt
    44221 gcactggccg cagcgcccga ccaactgctt cggcaaactc ggttatagct ggacggattc
    44281 tgcgcctgcg gtttcgctgc tggatacgct ggacgcactg gcagagtacc aacgcgcggg
    44341 aaaaattcgt tatatcggcg tgtcgaacga aactgcattt ggcgtaatgc gctacctgca
    44401 tctggcggac aaacacgatc tgccgcgtat tgtcaccatt cagaaccctt acagtctgtt
    44461 aaaccgcagt tttgaagtag gtctggcaga agtcagccag tatgaagggg tcgaactgct
    44521 ggcctattcg tgcctgggtt tcggcacgct gaccgggaaa tatctcaatg gtgcaaaacc
    44581 cgctggcgca cgtaatacgc tctttagtcg gttcacccgc tatagcggtg agcaaacgca
    44641 aaaagccgtc gcggcgtatg ttgatatcgc cagacgtcat ggccttgatc ctgctcagat
    44701 ggcgctcgcg tttgtacgcc gtcaaccgtt tgttgccagc actctgctgg gcgcaaccac
    44761 gatggatcag ctgaaaacta acatcgaaag tttgcatctg gagttaagcg aagacgtatt
    44821 agctgaaatt gaagcggtac atcaggttta tacttatcct gcaccataaa gattaacgcc
    44881 ggttaaaacc ggcgttaaat attaatgacg gcgctgccag atccacagcg ccgttattgc
    44941 cagcgcaaat agcgcaccga agccaatgcc aatgggcacg accgggatgc ctaccattac
    45001 cgccagcgag taaatgccca gcatcaacaa catggcgctg ttttcgccaa ggttttgtac
    45061 tgcaatcgca ttccccgccc cgacgctttt tttaccccgc tcctgtagca acgcattgag
    45121 cggaacgaca aaaaagcccc ccagcacgcc aatcagcatc aacaaggcat aggctggcag
    45181 cagctcgtgt tgcagggaga aaatcggtac caccacgcca atcaaaatcc cggctggcat
    45241 acagcgtgac acggtttcca gcgtaaccag cttcgctgcc gcacctgcgc caaccacgat
    45301 accaatcgct accatcgcgt tgagataggt cggcgtagcg ttatcggtaa tgcccagcgc
    45361 caccggtacc cacagcacca acaggaaacg cagcgtgaca cccgctcccc agaataaact
    45421 ggtgcccacc agcgaaaaac gcgtttcacc attgcaccat agcgaggtgc aggcattcag
    45481 gaaactgcgg gtcatgttga tgagattcca ggactgcccc ggacgcgccg ccagtttggg
    45541 aatgtagata ttggcaacga ccgcaccacc gtaggccagt gcgcatgcgg ccagagcgac
    45601 caggacatgc cagtcagcca gcacaccacc ggctacggaa ccgagtaaaa tcgccgctat
    45661 ggcagaagct tccattaaac cgttagcttt tactaactta ctacccgtgg ttaattcgcc
    45721 gagaataccg tatttcgccg gtgaataggc tgcagcacca acacccacca gcgtatagcc
    45781 gagaaacgga ttgataccaa agcagatact ggctgcgccc agcagcttca ggccgttggc
    45841 aaacatcatc acccggcctt tggcgaagct atccgccacc tgcccgacaa acggcgccaa
    45901 aagaatgtaa gcacctacaa acaccatttg caggatgggc tggctccact ccggatagaa
    45961 ctgcgctttc agtaacgcca gagtggcaaa cagtagcgcg ttatcgccaa acgcagagag
    46021 aaactgcgcc acgataaccg ctttcatccc cttcgaccac aacgaagtgt tagtgtgcac
    46081 tgactcactc atcgtgttgt tccgcttcgt ctacccagct tttcaacgtg acaaagtcag
    46141 gtttgccgct gccaagtaat ggcatctgtt tcagatagcg aatatcgcgc ggtacagcaa
    46201 gctccggcac gccgtgctcg cgggcatact gttgcaactt atcgcgcgtc agttcgttat
    46261 ctgtggtgaa aagcaccagt gcctcgcctt tgctggcatc gctcttaatc gcagtggcat
    46321 ggactttatc tggcgaaaca ccaagtgcca gttgttccac catttccagc gacaccattt
    46381 cgcctgcaat tttggcaaag cgttttgcgc ggccctgaat ctgcacaaag ccctgctcgt
    46441 caaaacgcac aatatcgcca gtgtcatacc agccgcgctc catttcgccg cgaacattct
    46501 cggcggtggg cacttccagt acacctggct tctccacccg cagatagccg ttcattatgt
    46561 tcggcccttt cagttgcagg cgtccgccct cttcgatacc agggaccgac aacaggcgtg
    46621 catccattcc tggtagaata cgccctaccg taccgggttt cgccgccatc ggtacgttga
    46681 tagaaacgac aggcgcgcat tcggtcacac cgtagccttc aaggatgcgc aggccaaatt
    46741 tatcctgcca aagctgtttg gtactttctt gtagtttttc tgcgcctgcc accacatagc
    46801 gtagacgata gaagtcatac gggttggcga aacgcgcgta gtgaccgagg aaagtcgagg
    46861 taccgaacaa cacggtgcaa ctgcggtcat acaccaagtc cggcacaatg cggtaatgca
    46921 gcgggcttgg ataaaggaac acttctgcac ctgtaagcag tggcgtaaac aggcctaccg
    46981 tcagcccaaa ggagtgaaac agcggtaatg ccgacataaa gcgatcgttg gtggtgaagt
    47041 cggcaatcgt tttaatctgc tcgacgttcg ccagaatgct tttatggcta tggacgacgc
    47101 cttttggatg gccttcagaa ccggaggtaa agaggatcag cgcctcttct tccggctgct
    47161 gttttacctg cgccaggcgc ggcatcagca aatgagcgaa gatccatact ttgtcggcag
    47221 tggtgacatc tgcttttaaa tcttccagat agacccagcg cacctgagta agttgctccg
    47281 gcagatgcca gagtttgcct ttatcgagaa actggcggga agtgaagatg gttttgattt
    47341 cagccgccgt aatagcactg gtcagccctt ttaccccggc agtgtagttc atcattgcgg
    47401 gaatgcggcg acgcgcgatg gccccaaaaa tcactgccgc actgatgcct gcattgggca
    47461 gcattaagcc gatgcgttcg ccttcaacac tgtatttttc aaggatgcgt ccaacaaaca
    47521 gcgttttcgt aagcaatttg cgataggagt ctggggtaaa gttgacgtct tcgacacatt
    47581 tcttcccggc tccgaagcgg tacattgcac tcagtaaaga ttcgtacaga gtttcacgcg
    47641 ggcgcaccgc catacgcgct tccatcatta tttgatgcag catttcgcca gcgattttgc
    47701 gacggtcacg ggcacgcggc gcatccggca tcgccacctg cgttggaggc aaaatatgca
    47761 gagtaatttg cgggaacaag cgacgtttaa ccagaccttt caggcggctg aagtgcgtaa
    47821 gttccgcccc ttcaatacgc acaggaataa ccgttgcacc agacttcgcc gcgacaaaac
    47881 ccgcgccatc gtagattttc atcagcgagc ctgtagtggt gatgcgtcct tcagggaaaa
    47941 tcaccactgg tcggccctgt tccaccagac gtaccagatg tttaatagcc ataggttgcg
    48001 tcgggtcgag aggaacaaag tcgataaatg atttcagcca acgcatatac cactgttggc
    48061 ttattgaggt gtaaacggca aacactggac gcacaggtaa aaacagtcca agcaaaatgc
    48121 catcaataaa agagacgtga ttaggcgtaa ttagtacgcg ctcgcccttc agtgcctggg
    48181 tgtcacccgt aacgcgaacg cgatacaaaa cacggcacaa atttcgaaaa aagctaaaaa
    48241 gcattccaac tccctttgct ctgattcagt aaaagcgaat ggagggagat tacacgagat
    48301 aaagaacgcg agcgacagta aattaggtgc gaaaaaaaac ctgcgcatcc gcgcaggttg
    48361 gtgcaagaga cagggtacga agagcgtacc gaataatctc accaatcaat acctctggga
    48421 tcttgattgt ggtctgcacg acgactcttc gccagcgaga aaacgcaaag gaatgaaggg
    48481 aaatgcaacg aggtgtgtaa attgtcggtt actgttacag attgatgacc ggcaaaaaaa
    48541 aacctgcgca tctgcgcagg ctggtgtaat tcatgtgctc aacacgaagt tgacttcacc
    48601 tatcaatacc tctgggatca ccactttagc aacctgaagc caaacgccac cagcggtcaa
    48661 tcgcaacagc gtttcgcaaa gtgtaaccaa aggtttgcaa tctctttttt tgtcttactt
    48721 atttgcgttt ggctcacatt cccacgatga aaacacgcca ccccttgaac caacgggcgt
    48781 tttccgtaac actgaaacaa tgtaagcgtt tacccactaa ggtattttca tggcgaccat
    48841 aaaggatgta gcccgactgg caggcgtttc agtcgccacc gtttcccgcg tcattaataa
    48901 ttcacccaaa gccagcgaag cttcccggct ggctgtgcat agtgcaatgg agtctcttag
    48961 ctatcacccg aacgccaacg cccgtgcgct ggcgcagcag accactgaaa cggtcggtct
    49021 gatcgttggt gatgtttccg atccgttttt cggtgcaatg gtgaaagcgg tcgaacaggt
    49081 ggcttatcac accggtaatt tcttattgat tggcaacggt taccacaacg aacaaaaaga
    49141 gcgtcaggct attgagcaac tgatccgcca tcgctgtgct gcgttggtcg tccatgccaa
    49201 aatgatccca gatgccgatt tagcctcatt aatgaaacaa atgcccggta tggtgctgat
    49261 caaccgtatc ctgcctggct ttgaaaaccg ttgtattgct cttgacgatc gttacggtgc
    49321 ctggctggca acgcgtcatt taattcagca aggtcatacc cgcattggtt atctgtgctc
    49381 taaccactct atttctgacg ccgaagatcg tctgcaaggg tattacgatg cccttgctga
    49441 aagtggtatt ccggccaatg atcggctggt gacatttggc gaaccagacg aaagcggcgg
    49501 cgaacaggca atgaccgagc ttttgggacg aggaagaaat ttcactgcgg tagcctgtta
    49561 taacgattca atggcggcgg gcgcgatggg cgttctcaat gataatggta ttgatgtacc
    49621 gggtgagatt tcgttaattg gctttgatga tgtgctggtg tcacgctatg tgcgtccgcg
    49681 cctgaccacc gtgcgttacc caatcgtaac gatggcgacc caggctgccg aactggcttt
    49741 ggcactggcg gataatcgcc ctctcccgga aattactaat gtatttagtc cgacgctggt
    49801 acgtcgtcat tcagtgtcaa ctccgtcgct ggaggcaagt catcatgcaa ccagcgacta
    49861 accgcagtta aagcaattcc agcgccagta attcttcgat ggtctggcga cggcgaatca
    49921 accgcgcctg accattatca aacagaactt ctggtaacag cggacggcta ttgtagttgg
    49981 atgacattga tgcgccatat gcccctgtat catgcagtac cagataatca cctgccttca
    50041 cttccggcaa ggcgcgggtt tcaacatttc ccccttcctg ctgggtaaag acatcgcccg
    50101 attcacataa cggcccggcg acgacggttt ccaccgttgg cgcgtgttcc agagaacgac
    50161 catcagctgc cagggcactg atatggtggt aactaccgta cattgccggg cgcatcagat
    50221 cgttgaaccc ggcatcaacc agcacaaagt ggcggctacc catttgtttg acgctccgca
    50281 cctgagtaat taatacgcca gactgcgcta ccaggaagcg acccggttca atttccagtt
    50341 tcacagggtg gcccaaatgg cgagcgattt gctcacgcgc ggcattccac agaccataat
    50401 aatgttcggt atcaaccgcc tcttcaccct gttgataagg aatagaaagc ccaccgcccg
    50461 cagaaatagc ctgtaaatcc tgaccgaatt cgataacctg acgcaccata gcaccacaca
    50521 cctgctccag atgggcataa tcaacgccag aaccaatgtg catgtgaatg ccgactagct
    50581 gcagatgatg acgttgtatc acgtccagtg cggcgggcag atcggtgtac cagataccgt
    50641 gcttgctgtt ttcgccaccg gtattggttt tttggctatg tccgtgacca aaccccggat
    50701 taacgcgcag ccatacccga tgccctggcg aaacctggcc cagttggtcg agcgtatcaa
    50761 cagaacccgc attcaccgga atttgcaatt cactgacgcg ttcaagcgtc gcctgatcga
    50821 taacatctgc cgtaaaaaca atatcatcgg ggtgcgtttg cggattgtaa cccgccgcca
    50881 acgcacgctc tatttcgcct aacgagacgg aatccacttt cacgccctgc tcacgcatta
    50941 agcgcaaaat atgaatattg gaacaggctt tctgtgcaaa gcgcaccact tcaaactgtt
    51001 tcagcgctgc aatctgccga cgaataattt gcgcatcgta gacccacacc gggcagccaa
    51061 attcagcggg caaacgcagc agattttcgg cggtgagatc ggtatcggtg ctgaacagtg
    51121 aatgtggcat aacaaactcc agatgagtgc ttttttatga ttacgccaca tcataaaaag
    51181 aataaaaaat atcgatttat gtcgagtcta tgcaaaaatg atatggatta ccggattgcg
    51241 agagagcgct aatggccgcc gttaacttac gtcatattga aatttttcat gcggtaatga
    51301 ccgccggaag cctgactgag gcagcacacc tgctacacac ctcacagcca accgtcagcc
    51361 gcgaactggc gcgctttgag aaggtgatcg ggctgaaatt gtttgagcgc gtacgtggac
    51421 gattacatcc taccgtgcaa ggactgcgtc tgtttgaaga agtgcaacga tcctggtacg
    51481 gactggatcg cattgtcagc gccgcagaaa gtctgcgcga gtttcgccag ggagaactgt
    51541 ctattgcctg cctgccggtc ttttcgcaat cttttttacc gcagctcctg caaccctttc
    51601 tggcacgtta tcccgatgtc agcttaaata tcgtgcccca ggaatcaccg ctacttgaag
    51661 agtggctctc ggcccagcgt catgatttag gactcactga gacactccat acgcctacgg
    51721 gaacagaacg taccgaatta ctctctttag atgaagtgtg tgtgttacct ccgggtcatc
    51781 cgctggcggt aaaaaaggta ttaacgccgg atgattttca gggtgagaat tacatcagcc
    51841 tttcccgtac tgacagctat cgccagttgc tggatcagct atttactgaa catcaggtta
    51901 aacgacgcat gatcgtagaa acccacagcg ccgcgtcagt ctgcgcaatg gtacgggcgg
    51961 gggtaggtat ttcggtggtt aacccgctca ccgcactaga ttatgcggca agcggtttag
    52021 tggtgcggcg gttcagtatt gcggttccgt tcaccgtcag cctgatccgc cccctgcacc
    52081 gcccgtcatc agcgctggtt caggcgttta gtgggcattt acaagcgggg ttaccgaaac
    52141 tggtcacttc tcttgacgct attttgtcgt cagctacgac agcataaaag cgacagcatc
    52201 ctcggcatgg atcgccgcgg tatcaaacac aggcaaaaca ctgcgctctt ctggcaccag
    52261 taaaccaatt tctgtgcagc caaaaatgac gccctgtgcg ccctgttctg caaggcgagc
    52321 aatcacttgc gcataataag cgcgtgacgc ttcggtaaat tgccccagac acagttcttc
    52381 aaaaataatc tgattaattt tcgcccgttc atccgcttca ggaataagac aattgatgga
    52441 aaattgttcc gtcagccgcc cgcgataaaa atcctgttcc atggtgtaac gcgtacccag
    52501 cagcgccaca cgagtcattc cggccccggt aattgcacgt ccggtggcat ccgcaatgtg
    52561 taagaaaggc agagtgcaac gtgactcaat ggcatccgcc actttatgca tcgtattggt
    52621 acataacaca ataccttctg cgcccgcccg ctgtaagcca agcgccgcct cagccagaat
    52681 gtccccggtt ttatcccatt ccccgcgacg ctggcactct tctatttcat gaaaatcgac
    52741 gctatgtagc agcatttgcg cagagtgaag cccaccaagc cgctgtttaa tgccttcatt
    52801 tatcagacga tagtaaggaa tggtggattc ccagctcatt cctcccagca aaccaattgt
    52861 tttcattcct ctctcctgat gtgtgttacc gcaacaaggc tgaataagga acgagacaaa
    52921 cgcctcaacg gccaggtgcc caatctctat taacgaaaaa agggccggat gtacagcaca
    52981 tccggcccgt gaaatcagac gccgatattt ctcaacttct cgcctgccat cagtttgcgt
    53041 tcgatatgtt ccagcgtgac atttttggtt tccggaatga gccagaaagt aatacccaca
    53101 aacgcaatgt tcagcgcagt gtagagccag aacgtaccgg cagcgccaat gctatcaagc
    53161 agtgtcagga aggccgcgcc gataatcata ttcgacaccc agtttgtcgt cgtcgaacag
    53221 gtaataccga aatcgcggca tttcagcggc tgaatttcag agcacaggat ccacaccact
    53281 ggcgcggcgc tcatcgcata accggcaata cacatcatcg tcatgccaac agagagccag
    53341 gacaagccac tggaagccgt accgttatca aactgcatca ggcaatagcc cagcaccaga
    53401 gtgcctaacg ccatcacgct gaaaccaatt ttcagagccg gtttacgacc tgctttatct
    53461 accgtaaaga ccgcaatgaa ggtcgcgaac ataaaggtca gtcccacgac cagagtcgca
    53521 atcatctgtt gttctgtggt cgtaaagccc gccattttga agatacgcgg cgcgtagtac
    53581 atgatgatgt tcataccggt aaactgctgc atcgcctgca acaacatacc gagaaacaca
    53641 gcacgacgga cgttacggtt gatcttaaac agtgcccaac cgccctgttt taacttcagg
    53701 ctttcacgaa tttcgttgag ttcttctcgc gctttttccg acgtatcgcg cagcatacgc
    53761 aacacttctt ccgcctcaat atgacgcccc ttttccgcca gccagcgcgg gctatttggc
    53821 aggaagacca ccagaataat cagcagaact gctggtaaag caagaacccc caacattgcg
    53881 cgccagttac cgctataact gaacgctgta tcggataaaa acgccagcac gatgccgagt
    53941 gtgaccatca actggtacat actgatcatc ttaccgcgaa cgttttcact tgccatttca
    54001 gaaagataca gaggagcggt gtaagacgcg atcccgacag caatgcccag caccacacga
    54061 gcggcgatta acatctctac gctggtcgca aaagcggacc ctatagaacc gagtacaaac
    54121 aggatggccc ccgccatcag gctgtattta cgccccaggc ggaacgacag ccaaccatta
    54181 aacagcgcac caattgctgc gccgagcatc atgctactaa ccacccattc ctgcaaacga
    54241 ctggtcagca caaagtgatc ggtaatgaac ggcaacgctc cggcgattac gccgatatca
    54301 agaccaaata acaatcctgc gaccgcagca gctaccgaaa caaacatatt catacgccgc
    54361 gtatcacgca aagaacgtgg cgttaaagca gattccgtat tgatagtaac cattttttcc
    54421 tgccagcaga gagtaagaca ttgtgaaaaa atacgtgaac aattcacaca ggtgtcaggt
    54481 cggaaacagc ataaatatgg attaaattgc tgcgacatgc cgttatgtga tggatattcc
    54541 aattttaaaa ttaagttgaa ttattgagat tattattaac ctcctaattt taaagcagat
    54601 aaaattcata aagttcatta attgataatt gatatggatt atttcataac catgatatgg
    54661 attatgatga tctagagata taaaaaaccc tgccatgcgg cagggtcata aaagtaagag
    54721 gaatgaatta acgcgccagc caaccgccat ccacagcaat ggtataacca tttacataat
    54781 ctgaagcgct ggaggcaaga aacactaccg gccccatcag gtcactcggc agtccccaac
    54841 ggccagctgg aatgcgatcg agaatttccg cgctacgttg ttcatctgcc cgcagttgtt
    54901 gggtattgtt ggtcgccatg taacccggag ctatcgcatt aacattaatg ttgtgtttag
    54961 cccattcgtt tgccatcaat cgcgtcacac ccatcacgcc gctttttgat gcggtataag
    55021 aaggcacacg gatcccgccc tggaaggaga gcattgacgc gatattgata atcttgccgc
    55081 cattgccttg cgcgataaag tgtttcgccg ctgcctgaga catgaagaat acgctcttga
    55141 tattcaggtt catgacatcg tcccagtctt tttcgctgaa ctcgagagcg tcttcgcggc
    55201 gaatcaatcc ggcgttattc accaggatat caatatgacc aaattcagct accgcgcgat
    55261 ccagcagtcc aggaatgcca tcaatctttc gcagatcggc ggtcaggctt aaaaaacgac
    55321 gccccagcgc cgtgacctgc tcgatggttt cagtcggttc aacgatgtta atgccaacaa
    55381 tgtcacagcc cgcttgcgcc agccccaacg ccatcccctg gcccagccca gtatcacaac
    55441 cagtgacgac cgcaacttta ccttggagag aaaatgcact taaaatcata acaacacctt
    55501 attcgtttat gcccacaact agcgcaaatc tttaacgccc acatggtcca tatcattaaa
    55561 gacctggttt tcaccgacca tgccccagat aaaggtataa gctttggttc cgacgccgga
    55621 atggatcgac cagctcgggg agatcaccgc ctgctcgtta tgcatcacaa tgtgacgcgt
    55681 ttcttgcggc tgccccatca tgtggaaaac gcaggcgtca tcatccatat tgaaatagaa
    55741 ataaacttcc atccggcgct cgtgggtgtg acacggcatg gtgttccaca agttacctgg
    55801 agccagctcc gtcagcccca tactcaattg acaggtttcc agtacatccg ggacaaaata
    55861 tttgttaatc gtgcgacggt tactggtgag gttatcgcct aacgtgactg gagatacttc
    55921 gtccggtgtg acttttttgg tgggatacgt cgtatgcgcg ggtgcgcaat tgtaataaaa
    55981 cttcgccgga gtggcggtat caatactggc aaagacaact tcttttgcac ctttaccaac
    56041 atacagggcg tcgcggtgac cgatttcata gcattgacca tcgacagtaa tcgtaccggc
    56101 accgccaata ttgataacac ctaactcgcg acgttcaagg aaatagctta cgccgagttg
    56161 tttaccaact tccccgccaa cggaaaccgt tttagttatc ggcataatgc cgccaacaat
    56221 aattcggtca atgtggctgt aaaccatggt gtactcatcg gcgacaaata ccttttcaac
    56281 caaaaattca ttgcgcagcc cttgggtatc cagcgttttt gcgtgcgcac cgtggatgct
    56341 ctgtcttacg tccacatcaa cctccgataa tcagtgaagc gtgaaaatag aaaacgatag
    56401 tgaaacactg tttcgttttc tctataaagg gatgatatgc ccatgggcta gggttttcaa
    56461 ttaaaaataa aacaatgttt tatttttaat ttaaggatta aaaaaagtgc agtgtcgatc
    56521 acgaatatgt catgagaatg gaaaaatgag ggagcccaga aaatgtatgt cgacttgcca
    56581 gccatcatta ttcaacagat taatgacggc agatatttat cggtaaggag gaaatgcaga
    56641 gctactcgtc acgttcaatg gtcaatgcca cgccctggcc cccgccgata caaagcgttg
    56701 ccagcccttt gcgggcatta cgtttcacca tttcatgaac cagagaaacc aggattcggc
    56761 aaccggaagc gcctatcggg tgaccgagtg cgatcgcgcc accattgaca ttgacccgac
    56821 gctcatccca ctcaagcatc ttgccaaccg aaagcgcctg tgcagcaaac gcttcattag
    56881 cctcgataag atcgacatca gccaactgcc agcctacacg ctccaggcaa cggcgggtcg
    56941 catacaccgg cgcaattccc atcaatgccg gatctacacc aacgctggca aatgcgcgga
    57001 tgcgggccag cacgggtaaa ttcaacgctc gtgctttggc ttcgctcatc atcattaccg
    57061 cagctgcgcc atcgtttatg gatgatgcat tccccgcggt cacagaaccg agactatcaa
    57121 atgaaggatt taaacgggct aaaccttctg cactggcgtc agtgcgtggc tgttcatcgg
    57181 tatcaacaac caacgtctga ccgttacttt ggatcattac cgggacgatc tcatctttaa
    57241 atcgtccggc gtcaatcgcc gctcgcgctt tttgttgcga gctaagtgcg taagcatcct
    57301 gcaactgacg gctgatgcca tattcgcgag ccagattttc ggcggtgaca ccaatatgat
    57361 aatcattgaa ggcatcccac aacccatcat gcaccagact gtcaaccaac tggctattgc
    57421 caagctgttc accggtgcgg ctatcagtca gaacatgtgg tgcgcggctc atgttttcct
    57481 ggccacctgc gatgacaata tcagcctcgc cacactgtat cgcctgagta gccagatgca
    57541 gtgctttaag accggaaccg caaacgtcat taatcgtgat tgcagaaacg ctgttaggaa
    57601 gaccaccttt aatagccgat tgccttgccg gattctgccc tgcccctgca gtcaacacct
    57661 gaccaagaat tacttcatcc accgcatatg caggaacgcc ggtacgttct attaacgctt
    57721 tcacgaccag actaccaagt tccacggcgg aatgacccgc taacgcacca cgaaagcagc
    57781 cgataggtgt ccgtaacgcc ccgacaatca caacgtcttt catcacaacc tcgcaccaat
    57841 taacagtgca atagtaaatg attgttaatt acagttatct taattgttta aaaaaagtga
    57901 tttttatcac aaaggaaata tgcctgagca gtagtcagag tcataactgg cacgtaaggt
    57961 ttgcaaccac taacccacca atagatgggt agatagggcg ttaatctccc atacttaact
    58021 tggtttatgg taaattgccc tccattttgt ttaatttgta gatgatacgt tcagataatg
    58081 tctaatattt ggtctaaaga agaaactctg tgaagtttcg cgctctatgg cacagccgtt
    58141 gatgcaggca cgctattcct tcctattcag ttaggttcgg caggggctgt ggtcctgttt
    58201 attactgctc tggtcgcctg gcctttaaca tattggccac ataaagcctt atgccagttc
    58261 atcctctcat caaaaacatc agcaggtgaa gggataacgg gcgcggtaac acactactat
    58321 ggcaagaaga ttggtaatct gattaccacg ctgtacttca tcgccttttt tgtcgtcgtg
    58381 ttgatatatg cagtggcaat taccaactca cttacggaac agctggcaaa gcatatggtt
    58441 attgatcttc gcatccgtat gttggtgagt ctgggtgttg tattaattct gaatctcatt
    58501 tttctgatgg gacgtcatgc cactattcgg gtaatgggat ttttggtatt cccattgatt
    58561 gcctatttct tatttctttc catttacctt gtcggtagtt ggcaacctga tctattaaca
    58621 acccaggtag agttcaatca gaataccctt caccagatat ggatatcgat tcccgtgatg
    58681 gttttcgcct ttagccatac gcccattatt tctacgtttg ccatagacag acgtgaaaaa
    58741 tatggcgaac acgctatgga taaatgcaaa aaaattatga aagtcgctta tctcatcatc
    58801 tgcataagtg tactgttctt tgtctttagc tgcctgcttt ctattccatc ttcgtatatt
    58861 gaagcggcta aagaagaagg ggtcaccatt ttatcggcgc tttctatgct gccgaacgcc
    58921 ccagcatggt tgtcaatttc cgggattatt gtcgcagtag ttgcgatgtc gaaatcattc
    58981 ctgggtacgt actttggcgt tattgaaggt gccacagagg tcgtcaaaac aacactacag
    59041 caggttggtg taaagaaaag tcgtgcattt aaccacgcac tatcaattat gttggtatcg
    59101 ctgattacct tcattgtttg ttgcattaac ccgaacgcga tttcgatgat ttacgcgatc
    59161 agcggcccgc tcattgccat gatacttttc atcatgccta cgctgtcaac gtatctcatc
    59221 ccggcgctta aaccctggcg ttccatcgga aatctgatta cgctgatcgt gggtatcctg
    59281 tgcgtatcgg taatgttctt tagctaatat ccgttgtgga tggttcaaaa ttgctacaat
    59341 tttgaaccat tccatttttc ataatattca tttgttatta ttccgcccta tcacttctct
    59401 cgtcatatcc tagaattatc agttagttaa acacacaaag agatctgaac atcttaaata
    59461 agactattta agatgcataa cttagattcg caagatatct tctggtcatt caaaaacaat
    59521 ttgcgatata gatattctca taccatgaga tagttatctg gaccacgttc gaccatgcaa
    59581 gattaaatca gtgaaacata atcatattta ataacgagaa atgcattttt aaatatctta
    59641 atcagaatta tagcaaatac agattaatcg caatttttcc cagcaaaatt acaaaatcaa
    59701 ggtgtattga catcatcaat caccaatgaa attatgcaat tatatacgga tagggaggtt
    59761 cttaacatgg ggctttgtag tcgttataaa agtcttacat gcaatagttg ctcaatgcat
    59821 tgccaaataa tgccagaaga gtcaccgcgt ttgcagtact gc
//
LOCUS       DAJTQQ010000013        58396 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000013 DAJTQQ010000000
VERSION     DAJTQQ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.013"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..1101)
                     /locus_tag="OO309_000884"
     CDS             complement(<1..1101)
                     /locus_tag="OO309_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCR5846126.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGAL"
     gene            complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="OO309_000885"
     CDS             complement(1313..1660)
                     /gene="pspE"
                     /locus_tag="OO309_000885"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR5846127.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="OO309_000886"
     CDS             complement(1703..1924)
                     /gene="pspD"
                     /locus_tag="OO309_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR5846128.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="OO309_000887"
     CDS             complement(1933..2292)
                     /gene="pspC"
                     /locus_tag="OO309_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR5846129.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="OO309_000888"
     CDS             complement(2292..2516)
                     /gene="pspB"
                     /locus_tag="OO309_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR5846130.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="OO309_000889"
     CDS             complement(2570..3238)
                     /gene="pspA"
                     /locus_tag="OO309_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR5846131.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3405..4382
                     /gene="pspF"
                     /locus_tag="OO309_000890"
     CDS             3405..4382
                     /gene="pspF"
                     /locus_tag="OO309_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR5846132.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4502..5767)
                     /gene="puuE"
                     /locus_tag="OO309_000891"
     CDS             complement(4502..5767)
                     /gene="puuE"
                     /locus_tag="OO309_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR5846133.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5805..7085)
                     /gene="puuB"
                     /locus_tag="OO309_000892"
     CDS             complement(5805..7085)
                     /gene="puuB"
                     /locus_tag="OO309_000892"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR5846134.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7087..8574)
                     /gene="puuC"
                     /locus_tag="OO309_000893"
     CDS             complement(7087..8574)
                     /gene="puuC"
                     /locus_tag="OO309_000893"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR5846135.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="OO309_000894"
     CDS             complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="OO309_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR5846136.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="OO309_000895"
     CDS             complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="OO309_000895"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR5846137.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10284..11702
                     /gene="puuA"
                     /locus_tag="OO309_000896"
     CDS             10284..11702
                     /gene="puuA"
                     /locus_tag="OO309_000896"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR5846138.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11851..12015
                     /locus_tag="OO309_000897"
     CDS             11851..12015
                     /locus_tag="OO309_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846139.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12005..13390
                     /gene="puuP"
                     /locus_tag="OO309_000898"
     CDS             12005..13390
                     /gene="puuP"
                     /locus_tag="OO309_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR5846140.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13524..13769
                     /locus_tag="OO309_000899"
     CDS             13524..13769
                     /locus_tag="OO309_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR5846141.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14082..15725
                     /gene="sapA"
                     /locus_tag="OO309_000900"
     CDS             14082..15725
                     /gene="sapA"
                     /locus_tag="OO309_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR5846142.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15722..16687
                     /gene="sapB"
                     /locus_tag="OO309_000901"
     CDS             15722..16687
                     /gene="sapB"
                     /locus_tag="OO309_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR5846143.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16674..17564
                     /gene="sapC"
                     /locus_tag="OO309_000902"
     CDS             16674..17564
                     /gene="sapC"
                     /locus_tag="OO309_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR5846144.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17564..18556
                     /gene="sapD"
                     /locus_tag="OO309_000903"
     CDS             17564..18556
                     /gene="sapD"
                     /locus_tag="OO309_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR5846145.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18558..19364
                     /gene="sapF"
                     /locus_tag="OO309_000904"
     CDS             18558..19364
                     /gene="sapF"
                     /locus_tag="OO309_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR5846146.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19432..19785
                     /locus_tag="OO309_000905"
     CDS             19432..19785
                     /locus_tag="OO309_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR5846147.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20153..20941
                     /gene="fabI"
                     /locus_tag="OO309_000906"
     CDS             20153..20941
                     /gene="fabI"
                     /locus_tag="OO309_000906"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR5846148.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDTSIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21085..22212
                     /locus_tag="OO309_000907"
     CDS             21085..22212
                     /locus_tag="OO309_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR5846149.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22280..24214
                     /gene="rnb"
                     /locus_tag="OO309_000908"
     CDS             22280..24214
                     /gene="rnb"
                     /locus_tag="OO309_000908"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR5846150.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24292..24395)
                     /locus_tag="OO309_000909"
                     /pseudo
     CDS             complement(24292..24395)
                     /locus_tag="OO309_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24449..26423
                     /gene="pdeR"
                     /locus_tag="OO309_000910"
                     /pseudo
     CDS             24449..26423
                     /gene="pdeR"
                     /locus_tag="OO309_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26571..26744
                     /locus_tag="OO309_000911"
     CDS             26571..26744
                     /locus_tag="OO309_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846151.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26834..27583
                     /locus_tag="OO309_000912"
     CDS             26834..27583
                     /locus_tag="OO309_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5846152.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27852..28070
                     /gene="osmB"
                     /locus_tag="OO309_000913"
     CDS             27852..28070
                     /gene="osmB"
                     /locus_tag="OO309_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR5846153.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28196..28522)
                     /gene="yciH"
                     /locus_tag="OO309_000914"
     CDS             complement(28196..28522)
                     /gene="yciH"
                     /locus_tag="OO309_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR5846154.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28522..29259)
                     /gene="pyrF"
                     /locus_tag="OO309_000915"
     CDS             complement(28522..29259)
                     /gene="pyrF"
                     /locus_tag="OO309_000915"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR5846155.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29452..30621)
                     /gene="lapB"
                     /locus_tag="OO309_000916"
     CDS             complement(29452..30621)
                     /gene="lapB"
                     /locus_tag="OO309_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR5846156.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30628..30936)
                     /gene="lapA"
                     /locus_tag="OO309_000917"
     CDS             complement(30628..30936)
                     /gene="lapA"
                     /locus_tag="OO309_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR5846157.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31085..31849)
                     /gene="pgpB"
                     /locus_tag="OO309_000918"
     CDS             complement(31085..31849)
                     /gene="pgpB"
                     /locus_tag="OO309_000918"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR5846158.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32019..32609
                     /gene="ribA"
                     /locus_tag="OO309_000919"
     CDS             32019..32609
                     /gene="ribA"
                     /locus_tag="OO309_000919"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR5846159.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32673..35348)
                     /gene="acnA"
                     /locus_tag="OO309_000920"
     CDS             complement(32673..35348)
                     /gene="acnA"
                     /locus_tag="OO309_000920"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR5846160.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35512..35607)
                     /locus_tag="OO309_000921"
     CDS             complement(35512..35607)
                     /locus_tag="OO309_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846161.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35721..35888)
                     /locus_tag="OO309_000922"
     CDS             complement(35721..35888)
                     /locus_tag="OO309_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846162.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35891..36055)
                     /locus_tag="OO309_000923"
     CDS             complement(35891..36055)
                     /locus_tag="OO309_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR5846163.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(36350..37324)
                     /gene="cysB"
                     /locus_tag="OO309_000924"
     CDS             complement(36350..37324)
                     /gene="cysB"
                     /locus_tag="OO309_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR5846164.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37534..40131)
                     /gene="topA"
                     /locus_tag="OO309_000925"
     CDS             complement(37534..40131)
                     /gene="topA"
                     /locus_tag="OO309_000925"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR5846165.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALELQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40511..40762
                     /locus_tag="OO309_000926"
     CDS             40511..40762
                     /locus_tag="OO309_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR5846166.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40798..41847)
                     /gene="sohB"
                     /locus_tag="OO309_000927"
     CDS             complement(40798..41847)
                     /gene="sohB"
                     /locus_tag="OO309_000927"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR5846167.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            42067..42825
                     /locus_tag="OO309_000928"
     CDS             42067..42825
                     /locus_tag="OO309_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR5846168.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42822..43412
                     /gene="cobO"
                     /locus_tag="OO309_000929"
     CDS             42822..43412
                     /gene="cobO"
                     /locus_tag="OO309_000929"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR5846169.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(43452..44324)
                     /gene="rluB"
                     /locus_tag="OO309_000930"
     CDS             complement(43452..44324)
                     /gene="rluB"
                     /locus_tag="OO309_000930"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR5846170.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(44425..45045)
                     /locus_tag="OO309_000931"
     CDS             complement(44425..45045)
                     /locus_tag="OO309_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR5846171.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(45042..45923)
                     /gene="yciV"
                     /locus_tag="OO309_000932"
     CDS             complement(45042..45923)
                     /gene="yciV"
                     /locus_tag="OO309_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR5846172.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            46061..46105
                     /gene="trpL"
                     /locus_tag="OO309_000933"
     CDS             46061..46105
                     /gene="trpL"
                     /locus_tag="OO309_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR5846173.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    46082..46175
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            46197..47759
                     /gene="trpE"
                     /locus_tag="OO309_000934"
     CDS             46197..47759
                     /gene="trpE"
                     /locus_tag="OO309_000934"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR5846174.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            47759..49354
                     /gene="trpD"
                     /locus_tag="OO309_000935"
     CDS             47759..49354
                     /gene="trpD"
                     /locus_tag="OO309_000935"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR5846175.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            49355..50716
                     /gene="trpCF"
                     /locus_tag="OO309_000936"
     CDS             49355..50716
                     /gene="trpCF"
                     /locus_tag="OO309_000936"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR5846176.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            50728..51921
                     /gene="trpB"
                     /locus_tag="OO309_000937"
     CDS             50728..51921
                     /gene="trpB"
                     /locus_tag="OO309_000937"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR5846177.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51921..52727
                     /gene="trpA"
                     /locus_tag="OO309_000938"
     CDS             51921..52727
                     /gene="trpA"
                     /locus_tag="OO309_000938"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR5846178.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            53107..53286
                     /locus_tag="OO309_000939"
     CDS             53107..53286
                     /locus_tag="OO309_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR5846179.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            53372..53872
                     /locus_tag="OO309_000940"
     CDS             53372..53872
                     /locus_tag="OO309_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5846180.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            53918..54424
                     /locus_tag="OO309_000941"
     CDS             53918..54424
                     /locus_tag="OO309_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5846181.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(54484..55122)
                     /gene="ompW"
                     /locus_tag="OO309_000942"
     CDS             complement(54484..55122)
                     /gene="ompW"
                     /locus_tag="OO309_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR5846182.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIAIVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            55362..55451
                     /locus_tag="OO309_000943"
                     /pseudo
     CDS             55362..55451
                     /locus_tag="OO309_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            55479..56222
                     /locus_tag="OO309_000944"
     CDS             55479..56222
                     /locus_tag="OO309_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR5846183.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            56252..56791
                     /locus_tag="OO309_000945"
     CDS             56252..56791
                     /locus_tag="OO309_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR5846184.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            56896..57294
                     /gene="yciA"
                     /locus_tag="OO309_000946"
     CDS             56896..57294
                     /gene="yciA"
                     /locus_tag="OO309_000946"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR5846185.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(57334..58062)
                     /gene="tonB"
                     /locus_tag="OO309_000947"
     CDS             complement(57334..58062)
                     /gene="tonB"
                     /locus_tag="OO309_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR5846186.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            58277..>58396
                     /locus_tag="OO309_000948"
     CDS             58277..>58396
                     /locus_tag="OO309_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846187.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTA"
BASE COUNT        14019 a        15104 c        14588 g        14685 t
ORIGIN      
        1 taatgcacct tcctgctgta atgcctcgac cagcgctaat atttcgcttt ctggaagcaa
       61 gccacgcagt gggttcaacc cgatgccatc gtgagaggcg aggaagttaa accaggtcgt
      121 tttactggag ggcaagctca gactttgcgc ccactggcac agcgcctcga cgttctgttt
      181 ttgcaccgca tgtaacacca gcggcggcag cgagaactgg tacaccatat gcgcttcgtc
      241 atcaccgttg ccaaagtaag caatgttgtc tttgtgcgga acattggttt cggtaataat
      301 cactgtacct ggtgctacgt catcaataat cgaccgtaac agcttgataa tcagatgtgt
      361 tttttccaga tggatgcagc ttgttcccgg ctctttccac ataaagccaa cggcatccag
      421 gcggacatac tcagcacctt tctcaaggta acagagtaaa acatccacca tcgccagcaa
      481 cacttcaggg ctacggtaat tcaggtcaat ttgatcgtca ctaaaggtgg tccataaatg
      541 gcgcgtggaa ttatcatcca tttgaaacgg cgttaatagc ggtaacgcac gcggaagggt
      601 gacggcactg agatcggttt tcggatcaac cgcaataaag aaatcttcaa aacccggaag
      661 ctgttgtaaa tagtttttaa accattcact ttttgcagac atatggttgc agacaaaatc
      721 aaacattaaa tgactgcatt caccgagttg ctgaatatcc tgccactccc ccgcttcact
      781 ggcgacctga tgataatcaa ttaccgaaaa tccatcatca gatgaccatg ggtaaaaagg
      841 taataaatga acatgtgaaa aaatgctttg cagccattga tggtagaact gattaaatat
      901 gggtagtggt tttaaatcat tactgtgaaa ttgatcggca taggtaatta acacgacatc
      961 actttcatcc cagtgttttt tacgtcgctg tgtaattaat cgtttcgcac tctcaagacg
     1021 cgttacaagt ttctgtaaat gagttgcggt aaatgttcca ccgtagattt cgtccatgta
     1081 atccgtaatt ttctgtttca tgttgccttt ccgtggtagc ggtcccatga ggcgagagac
     1141 tactccgcag aaaataaact gtcaattgag cacaagggtt ttagtcacgc aggaaatcat
     1201 tttttaccag ggaaaaagcg tatttgtgag ctgacgcaaa taatctatag ggatgtaaat
     1261 aaggtgtgac agaaaaaaaa cggcgcataa gcgccgctca tggtgaattc ttttaacctt
     1321 tgaccttcgg cattgcgatg tctttcaggc caccggcgtt ctcaacgtgg gtatatccca
     1381 tctcgctaag gagctctttt gcctgcccgg actggcgtcc ggcattgcaa tacactttca
     1441 cggtgtcgtt tttatccgga acggcggtgg caatgcgctc tttcacttcc ttcaggggaa
     1501 tattgatggc cccctgaacg tgctcttgct gatactgctc tggaacacga acatcgatcc
     1561 agtgttcagc ggcgaaaacg ggcagtgaaa acaccagtgc cagagcaagt aagccttttt
     1621 ttaaacatgt aagcgtcctt ctatggatga aaattgccat ccattatacg cctgagattt
     1681 aacaattttt gcgaaactcc cctcaccttt tataacgctg tgccagttta ttagcagccc
     1741 gacttaacag cggttccagc gccacagcca gcaacatttt cagcggtcgg cgagcaactg
     1801 attttatcgc ccagcccgcc acacccgccg ggccatagcg cagtgcggta agaagtacca
     1861 gcttgcctgc taatttgaaa ccaagcttca ccttttgccc ggcctgttgc cagcgagtat
     1921 tcataacttt cctcacagtt gacggaaacg gctacgtaac gtgaaagtat cggaagtgac
     1981 ataacgttcc atctcgcgta aacgcgtttc acttgccgcc agttcacggt cgacttcatc
     2041 cagcaattcg ctgctggaag gtagctgctc accaaaggcc atgttgtccg gcattggatc
     2101 aagcgcaaat gacaaaatga tgtaagcaac cagggtaaac agcgccagac cgaaaaaaat
     2161 cgacagcacc accaggatac gcaccagttt taccggtaca tcaaaatagt tggcaatccc
     2221 ggcgcagacg ccgcggacca tgccctgctg tggaatacgc cataactttt tattgagatt
     2281 aatgcccgcc attagcgatc cctccagttc ggatgttctg catcaagaat agattccagc
     2341 gcctgaatac gttcgcgcat ccgttttgct tcatcaacca gttgcgctaa tcgctgctgc
     2401 tcactttgcg acaattcact gcgaccagaa cgattgctgt aatgcagcca taaccagatc
     2461 ggtaaaacaa acagcacaaa aatggttaac ggaatagcca gaaatagcgc gctcataagt
     2521 actccttaga cgatgagcgg cggcgcgttc ggacgccgcc tggatgttat tattgattgt
     2581 cttgcttcat tttggctttt aattgtgcca gttgttcgct gattgcatca tcggctttca
     2641 gttcggcaaa ctgatcgtcc agcgattttt gtttaccgaa gctgtggctt tctgcttccg
     2701 cttccatctg gtcaatacga cgttcgaaag attcgaaacg agccattgct tcatccagtt
     2761 tgccactgtc cagctgacga cgcacatcgc gcgacgagtt tgccgcctga tgacgtaaca
     2821 tcaatgcctg ctggcgagcg cgtgtttcgc tcaatttgtt ttccagctcg ccaatctctt
     2881 tcttcatgcg tgccagcgta tcgtccacca gcgtcacttc atgttccagg gacttaatca
     2941 gatcggtcag tttctgtttt tcaattaacg ctgcacgtgc cagatcctct ctctctttca
     3001 gcagcgccag ttcggctttt tcctgccatt caacttcacg cgccgacgct tgttcaatac
     3061 ggcgagtcag ctgtttcttt tctgccaacg cacgcgccga agtagaacgt acttcaacca
     3121 gtgtatcttc catctcctgg atcatcagac gcaccagttt ctgtggatct tccgctttct
     3181 ctaacagagc gttgatgttg gcattcacga tgtcggcaaa gcgagaaaaa atacccataa
     3241 tgttgtcctc ttgatttctg cgttcaggat tgtcctgctg aactgttaat acaatttgcg
     3301 tgccaatttt ttatcttttt gatttataaa gatctgattg aagaatcaac agcaacatgc
     3361 caggatgaat tagcgaatta cactaacaag tggcgaattt catcatggcc gaatacaaag
     3421 ataatttact tggtgaggcg aacagctttc tcgaagtgct ggaacaggtt tcgcatctcg
     3481 caccgctgga caaaccggtg ctcatcatcg gcgaacgcgg caccggtaaa gagttgattg
     3541 ccagccgcct gcattatctc tcctcccgtt ggcaagggcc gtttatttcc cttaactgcg
     3601 cggcgttaaa tgaaaatctg ctggattccg aactgtttgg tcacgaagcg ggggcgttta
     3661 ccggtgcgca aaaacgtcat cctgggagat ttgaacgtgc cgacggcggt acgctatttc
     3721 ttgatgaact cgctacggca ccgatgatgg tgcaggaaaa attattgcgt gtgattgagt
     3781 acggtgaact ggagcgcgtt ggcggtagcc agccgttgca ggtgaatgtg cggttggtat
     3841 gcgcgacgaa tgccgatctc ccggcgatgg tcaatgaagg tacttttcgc gctgacctgc
     3901 tcgaccggct ggctttcgat gtagtgcaac tgccgcccct gcgcgagcgc gaaagcgaca
     3961 ttatgctgat ggcagaacac tttgccattc agatgtgtcg ggaaatcaag ctgcctctgt
     4021 tcccggggtt tacggagcgc gccagagaaa cattgctgaa ttatcgttgg ccgggaaata
     4081 ttcgtgaatt gaaaaacgtg gtggaacgtt cagtgtatcg ccacggcacc agcgattatc
     4141 cgcttgatga catcattatt gatcccttta aacggcgtcc acctgaagaa gccatcgccg
     4201 tttcagaaaa tacctcgctt ccgacgctgc cgctggattt acgcgagttt cagatgcagc
     4261 aggaaaaaga gttgctgcaa ctcagtttgc aacaggggaa atataaccag aagcgcgcgg
     4321 ctgaattact ggggttaact tatcatcagt tccgcgcgtt gttgaaaaag caccagattt
     4381 agcgaagttg agcaatacaa cttgccggat gcggcgtgaa tgctttattc ggcatacata
     4441 atcaatacgt cgtttgcagg cctgataagc gcagcgcatc agacattatt gcgttgggct
     4501 attaatcgct cagcgcatcc tgcaaaattt tcattgccgc atcgaattgc gcatccggga
     4561 tggtcagcgg atacaggaag cgaatcacgt tgccgtatgc gccacaggtc agcaggagca
     4621 gcccctgcgc cagcgcgcgt tgctggattt tctgtgcaat cgccgctgac ggctcgcccg
     4681 tttgcggatc gttaaactct gccgcaatca tcgaccccag gccgcgtacc gcagcaatgg
     4741 ccggaacgct ttctttggca tcaatcaacg tgtttttgag acgctggccc agttgattcg
     4801 cgcgttcgca gagtgattct ttgtcgataa tattgagcac cgcgtgcgcg gcagccaccg
     4861 ccagcgggtt cccggcgtag gtgccaccca acccgcccgg cgcgggcgcg tccataatat
     4921 tcgcgttacc gaccacgccc gaaagcggca tcccgcccgc gaggcttttc gccatcgtca
     4981 ttaagtccgg cttatcggcg taatgatcca tggcaaacag tttaccggta cgcgcaaagc
     5041 cgctttgcac ttcatcggca atcatcacaa taccgtgctc gtcgcacagg cggcgaatgg
     5101 cggcaaccag ctcttttggc gcaacgttga aaccgcactc gccctgcacc ggttcgaaaa
     5161 taatggccgc gacctgcttc gcttcgatgt ctgatttaaa caagcgttcg atggcgtcga
     5221 gggagtcctg tgttgaaatg ccgtgtaaat ctgacggata aggtacgtga tacaccgaac
     5281 cagggaacgg gccgaagccg attttgtacg gcgcaacttt tccggtcagc gccatggtca
     5341 tatatgtacg accgtggaag ccaccgctaa acgcaatcac gccaggtcgt ccggtatggg
     5401 cgcgagcaat tttcaccgcg ttttccaccg cttccgcgcc ggtagtgaag aatgcggttt
     5461 tagcctgccc gctcactggg gcaagggtgt taattttctc cgccagcgtg acgtagcttt
     5521 cgtacggcac aatctgatac gccgtgtggg taaactgttg caattgctgc tccaccgccg
     5581 cgaccagatc agggtggcga tgcccggtat tcagcaccgc aatgcctgcg gcgaaatcga
     5641 tgtattcgtt gccctctacg tccttcagcg tagcgttttc tgccgactgg gcgaagaagt
     5701 tacacatcac gccaaccccg cgtggggtag cagaaagacg acgctgatgg aattcattgt
     5761 tgctcatgtt tcacaccttt tgactcttca ctgttaatcg gaaattaaac gcccagacga
     5821 tcgcgcaggc tgtaataagc cgcgcccatc gcggtaaatg gcacgcgcag cgtgcgcccg
     5881 ccggggaacg ggtaatgcgg cagattggcg aaggcatcga aacgctcggc gtcaccgcgc
     5941 agcagttcag caatcaaacg cccggcaaga tgggtacagg tgacgccgtg gccgctgtaa
     6001 ccctgcatgt aatagatgtt ggtatcaagg cggccgaact gcggcatgtg cgacaaggtc
     6061 agcaggaagt tgcccgtcca gcggtaatca attttcacgc ccttcaactg agggaaggtt
     6121 tttagtagtt tcggtaccac aagacgctca acgtcatccg ggttgcgtgc accatacact
     6181 acacccccgc cgtacagcag gcggttgtcg gcggtaagac ggtagtaatc aagcagatag
     6241 ttacagtctt ccacacagta gtttttcggg atcagcgaac gggctaaatc ttccgacagc
     6301 cgttcggtgg tgatcacctg cgtgccgcac ggcatgctgc gtttcgctag ttccggctct
     6361 actttatcgc ccagatacgc attcccggcg acaatcacat acttcgccgt cacctgacca
     6421 ttggcagtcc gaaccacggc tggcgtggtg tgctggatct gcgtcacggc ggaaagctca
     6481 tacacgcgcc cgccgttcag gcggatggca tcggcttcac caatggcgag gtttagcggg
     6541 tgaatatgcc caccgctgtg atccagcagc gcaccagtgt agcgatcgct ggcgacctcg
     6601 cggcgaatag cgttgacgtc cagcagctcc agctgtttat tgccgtagcg tacccagttc
     6661 tctttctgct cttcgagtgt ggcgagttgt ttatcattca tcgccacaaa cagaccgccg
     6721 gggcggtagt cgcagtcaat ctgataacgt ttgatacgtt cgcggatgat ctcgccaccc
     6781 tcgaacatca tgctgccgag catacgggcg gtgtccatgc cgtagctttt ttcgatcacg
     6841 tcgatgtcgc ggctgtagga gttcacaagc tgcccgccat tgcgcccgct tgcgccaaag
     6901 ccgatgcgtg aggcttcgag aaccactacg tcaaagcccg cttccgccag atgcagcgcg
     6961 gaggagagcc cggtatagcc gccgccaacc acgcaaacgt cgcaggtgat tgactcattc
     7021 agtgtgtcga atggtgcata tttattcgca ctggcggcgt agtaactgct ggtatgttcg
     7081 gtcatttcag gcctccaggc ttatccagat ggttttcagt tcagtgaatt tttcaagggc
     7141 atgcagggat ttgtcgcgac cgttgccgct ctgcttatag ccgccaaacg gcacggtcat
     7201 atcgccgtcg ttgtagttat tgacgaagac ggaaccggct ttcaggcgtc ggctcatgcg
     7261 gtgcgcgcgg gagaggtcgc gcgtccatac cgccgcgcca aggccgtact ggctgtcgtt
     7321 ggcaagctgc agcgcctgat cttctgatgt gaaacgggtg accaccagca ccggaccgaa
     7381 aatctcttcg cggcttaagg acgcattcgg gtccacatcc acgaagatgg tcgggccgat
     7441 ggccgctgcc agcccggcgt tgcgaccatc cagcagcagt tgccccttgc cttcgccttc
     7501 acgaataaag gtgtgtaccg agtcagcgtg agcgctgtcg attaaggtgc ccatggtggt
     7561 tgcgggatca agtggatggc ccggctgcca gttttgcgcc tgctgtttta acagggctaa
     7621 gaattcatcg gcgatgctct cttccagtaa caaacgcgtt ccggcgatgc gcacctggcc
     7681 ctggttgtag aagatgcctg cggcggtggc gcttgccgct ttttgcagat ccggacaatc
     7741 ggcgaaaacg atgttggcgc ttttgccgcc cgcttccagc cagacacgtt tcatgttgct
     7801 atcacccgcg tctttcagca gttgtttacc cgtacgcgtg gagccggtaa aggcgatggc
     7861 atcgatatcg ttatgacgcg aaagcgcctg ccccgcttca tgaccaaaac ccgtcaccac
     7921 gttcaacaca ccatccggca agcctgcttc tttcgccagc cccgcgagac gaatcgcact
     7981 gagcggtgat ttttcagacg gttttagaat cacgctgttt cccgccgcca gcgccgggcc
     8041 gagtttccag caagtcagca acagcgggaa gttccacggc acgatggcgg caatcacgcc
     8101 gaccggttca cgcacgatca tcgccagctc atggctactg gtggtcgcca cttcgccata
     8161 cactttgtcg atcgcttcgg cgtaccagcg aatggcgcgc gccgcgccgg gaatatcatc
     8221 acgcagactg tgacgaatcg gtttgccggt gtcgagagtt tccagcagtg ccagctcttc
     8281 ggcgtgggct tccattaaat cggcgagttt attcagtacc gctttacgtt tagccggaga
     8341 agagagtgac cagtcgccgc gttcaaatac gccgcgtgct gcgctcaccg cacggtcgat
     8401 atcgacgctc ttgccgcggg caattttcgc cagcggtgcc tgggtgaccg gatcaacggt
     8461 ttcaaaggtt tcattttccg ccgcagcagt atattcaccg ttaataaata agcggttttc
     8521 aatggcgaga cttaacgctt tatcctgcca gtaagccaga tgatgaaaat tcattatgac
     8581 tcctgtttca cgtctatcag atatatgcag tttgtgaacg tcagggcgca gagatccccg
     8641 ggctgcatgc ccgtttattt ctggcgacat tctttatgga ttgccgggcg cattgcgctt
     8701 acccggctta cagaacaaaa agattaaaac gtggtgggcg tatgggcgct gataattcgg
     8761 caaatacctg ccgacgtatt actgaaactg tgcgggatgc cggtattaat ggcataactt
     8821 tgccccgcga cgaggtggta atcctgacca ttaatcgtca gaacaatttc accttccagt
     8881 actgtgccta tttcctcacc ctgatgctta attctttccc cagtggttgt gcccggctgg
     8941 tacgtttcaa agatcatcgc cagcgtgcga ttcgggttac cgttatgaac cagcttcatt
     9001 gacacaccct gactgcccat ctcaattaag tcgtcctgat taatgacgac ctgcggctca
     9061 tcaggttttt ccggctcgga aaagaattcc gagagtgaca gaccatacac cttcagcagc
     9121 ttttgcagcg tactgatggc agggctgact ttatcttgtt ctatcgtact gatagcactg
     9181 tgagtcagcc cggagagttc ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt
     9241 tccgacaagc gttttcctgg cgccagtccc tcatcactca tatttgcatt tccttaaact
     9301 gtagtggtca gagtcgctgt ttttcagcga tatggtgctg ccaagcggtg ataaagccct
     9361 cgaacaatat acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc cactgtacgc
     9421 ccagcgcaaa aggatgattg atgacgctaa ccgcctcaac taagccatcc ggcgagcgag
     9481 cttcaacacg caaccgtggg ctaacgacct tcgccccttg tccatgtaga gagtttaccc
     9541 aaaagttgct acattcaggt aacaacgcag acagtaatcc cccctcttca acctgaactt
     9601 catgcgacgg tgcatattgc tgttccaccg gcagttcggg atcttcccga tgttccagca
     9661 attcaggctg ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc accagttctt
     9721 gtaaaccccg gcagatggcg aaaatgggga tgcgcctttc gagtgcggca ttaattatcg
     9781 ccatgctcag aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt tcaccatata
     9841 ggtgcggctg cacattgctg ggactactag gaagataaat gccatcgagt ttcggcaaaa
     9901 gttgttcaag taacgacggt tccgccagcg catgtggtag cgcaattggc aagccgcctg
     9961 cgtggatgat ggcattcagg tacttttctt gcagagtctg ggtcgcatga cccttaagcc
    10021 tgttcctgca cattacgaca ccgataaccg gattgttcat tatattttcc atgctcaatc
    10081 tcacaaaatg gactaaatta tcgccattac tgcgctttaa atgaccttta tgttcaatat
    10141 tttttcaatc tagcagtgga ttttcatttt tgcaaactca atttaacatt tgacaaacat
    10201 ttggtttgca tacagattcg aatgatggtc attatatttt acgctttgat aacgagcgga
    10261 aaacaaacca aaggcgaaga atcatggaaa ccaatatcgt tgaagtagag aactttgttc
    10321 agcagtcaga agagaggcgg ggtagcgcct ttacgcagga agtgaaacgc tacctggagc
    10381 gctacccgaa tacgcaatat gttgatgttt tacttactga tttgaatggt tgcttccgtg
    10441 gaaaacgtat ccctgtttca agcctgaaga agctcgagaa agggtgttat ttcccggcct
    10501 cggtatttgc aatggatatt ctgggcaacg tagtagaaga agcaggtctg ggtcaggaaa
    10561 tgggcgagcc ggatcgtacc tgtgttcctg ttctcggttc cttaactcct tctgccgccg
    10621 atccagagtt tatcggtcag atgctcctga ccatggtcga tgaagatggc gctccctttg
    10681 acgttgagcc gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag cgcggattgt
    10741 tcccggttgt agcggtagag ctggagttct atttactgga tcgccagcgc gacgctgaag
    10801 gggatctgca accgccctgc gcgcctggca ccgatgaccg caatacacaa agccaggttt
    10861 actccgttga taacctcaac cacttcgccg acgtgctcaa tgatattgat gaactggcgc
    10921 agttgcagct gattccggca gatggcgcgg tcgctgaggc ctcgccgggt cagtttgaaa
    10981 tcaaccttta ccatactgat aacgtgctgg aagcctgcga tgatgcgctg gcactaaaac
    11041 gcctagtgcg tctgatggca gaaaagcata agatgcacgc cacttttatg gcgaagccgt
    11101 atgaagagca cgcgggcagc gggatgcata tccatatcag tatgcaaaat aatcgtggcg
    11161 agaatgtgct ttctgacaca gaaggcgaag attcgccgct gctgaaaaag atgctcgccg
    11221 ggatgattga cctgatgccg tcgtcgatgg cgttgctggc accaaacgtg aactcgtatc
    11281 gccgcttcca gccgggaatg tatgtgccga cgcaggcgtc gtggggccat aacaaccgca
    11341 ccgtcgccct gcgtattccg tgcggtgacc gtcataatca ccgcgtggaa tatcacgtgg
    11401 cgggtgccga tgccaaccca tatctggtga tggcagcgat ttttgccggt attttgcatg
    11461 gccttgataa cgagctgccg ttgcaggaag aagtcgaagg caacgggctg gaacaggaag
    11521 gcttaccctt cccgattcgc cagagcgatg ccctgggtga gtttatcgag aatgatcacc
    11581 tgcgccgcta tttaggcgaa cggttttgcc atgtgtatca cgcctgtaaa aatgatgagc
    11641 tgttgcagtt tgagcgcctc atcacagaaa cagaaattga gtggatgttg aaaaacgcgt
    11701 aattcccctt gcggggttgt ttcatcggcc tgagcggagc caggcctgac atttgctggt
    11761 aatccagaca tttcccggat gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa
    11821 cgattaagac tgtgacgagg aaatgagatg atgccgatga ttaaatcccc gcatggcgag
    11881 ggaggctgcg tatgcgcccc tcctgctact gactggactc cgcccccttt gcttcccttg
    11941 ctaaacaggt ttgatttcag gtcgacacga ccgcaaacct tattacgcag gggaggcagc
    12001 aattatggct attaattcac cactgaatat tgctgcgcaa cccggcaaaa cccgtctgcg
    12061 aaaatcactg aaattgtggc aggtggtaat gatgggtctg gcctatctca cgccgatgac
    12121 cgtatttgat acttttggta ttgtctccgg cattagcgac ggtcacgttc cggcgtccta
    12181 tttgctggcg ctggcgggcg tgctgtttac cgctatcagc tacggcaaac tggttcgcca
    12241 gtttccggag gccggttcgg cctataccta cgcgcaaaag tcgattaacc cgcacgtcgg
    12301 atttatggtc ggctggtcat cactgctgga ttatctcttt ttgccaatga tcaacgtttt
    12361 gttggcgaaa atctatctct ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt
    12421 aaccttcgtc gccattttaa ccgccgcgaa tctgaagagc gtcaacctgg tcgctaactt
    12481 caataccctg tttgtactgg tgcaaatctc catcatggtg gtgtttatct tcctggtggt
    12541 tcagggactg cataaaggag aaggcgttgg caccgtctgg tcacttcagc cgtttatcag
    12601 cgagaacgcg cacctgatcc cgattattac cggtgcgacg attgtctgtt tctcgttcct
    12661 cggtttcgat gcggtgacca cgctttcgga agagacgccg gacgccgcac gcgtgatccc
    12721 gaaagccatc ttcctgacgg cggtatacgg cggcgttatc tttatcgcgg cgtcgttctt
    12781 tatgcagctg ttctttcccg atatcagccg ctttaaagac ccggacgccg cactgcctga
    12841 aattgcgctg tacgtcggcg gcaagctctt tcagtcgatt ttcctctgca ccacgtttgt
    12901 gaacacatta gcgtctggcc tggcttcaca tgccagcgtg tcgcgtctgc tgtatgtgat
    12961 ggggcgtgac aatgtgtttc cggagcgcgt gtttggctat gtgcacccaa aatggcggac
    13021 tccggcactc aacgtcatta tggtcgggat tgtcgcgctg tcggcgctgt tctttgattt
    13081 agtcaccgcg acagcactga ttaacttcgg tgctctggtg gcgtttacct tcgtgaatct
    13141 gtcggtgttt aatcatttct ggcggcgtaa aggaatgaat aaaagctgga aggatcactt
    13201 ccactatttg ctgatgccgc tggttggcgc gctgacggtg ggtgtgctgt gggttaacct
    13261 agagtcaacg tcactgacac tcggtctggt atgggcttcg ttaggcggcg catatttgtg
    13321 gtatctgatc cgccgctatc gcaaagtgcc gctgtacgaa ggtgacagaa cgccggtgag
    13381 cgaaacgtaa tagccggatg cgcggcgcat tatcctgcct gagggtaacg cgccgcgcca
    13441 ttaactataa aaagatgtat gacccggatc acgagtgcta tcatccgccc ttatgcttag
    13501 cgcattaacc ccaggggcaa ataatgaacc acgatatccc gctaaaatat tttgatattg
    13561 ccgatgagta cgcgaccgaa tgcgcagagc ctgtcgcaga ggctgaacgc acaccgctgg
    13621 cccactactt ccagttactg ctcacccgtt tgatgaacaa tgaagagatc agcgaagaag
    13681 cccaacatga aatggctgct gaagcgggga ttaatcccgt acgcattgat gagatcgcag
    13741 agtttttgaa tcaatggggt aatgagtaat tatttaattg cagacgatcg gtcagtatcg
    13801 ttgtccggat aaagcgttca cgccgcatcc ggctaacaat gacacgcact gcagcgtgct
    13861 ttaatgggtt tctcacgatc gtaccgctct gctcctcgct aagttgttct gtgagttata
    13921 gttatagaga aatcacgggt acgaatgttg aacagatggc gacgccacct gttcaacgac
    13981 caaccacacg gccctcatta cgacccctga acacaaacgg taatggcaaa tgtcattaaa
    14041 tattagtaag atagcctctt tacataaaat cccttaatat tatgcgccag gtattatcgt
    14101 ctcttttggt gattgctgga cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc
    14161 cgcatgctga tatccgcgac agcggttttg tctattgcgt cagcgggcaa gtcaacacct
    14221 ttaacccatc caaagcgagc agtgggttaa ttgtcgatac ccttgccgcc cagttttatg
    14281 atcgactgct ggatgtcgat ccctatacct atcgcctgat gccggaactt gccgaaagct
    14341 gggaagtact cgacaacggc gcgacctatc gcttccacct gcgtcgcgat gttccgtttc
    14401 aaaaaaccga ctggtttact cccactcgta aaatgaatgc cgacgatgtg gtgtttacct
    14461 tccagcgaat ttttgaccgc aacaacccgt ggcataacgt caacggcagc aacttcccct
    14521 acttcgacag cctgcaattt gccgataacg tgaaaagcgt ccgcaaactg gataatcata
    14581 ccgttgagtt ccgactcgcc cagccggatg cttctttttt gtggcacctc gcaacccatt
    14641 atgcttcggt catgtcggca gaatatgccc ggaagttaga gaaagaagat cgccaggagc
    14701 aactcgaccg tcaaccggtc ggcaccggtc cgtatcagtt gtcggaatac cgcgccgggc
    14761 aatttattcg cctacaacgt catgatgact tctggcgcgg taaaccgtta atgccgcagg
    14821 tagtggtgga tttaggctcc ggcggcaccg gacgtctgtc gaaactcctg accggggaat
    14881 gcgacgttct ggcctggcct gctgccagcc agctatccat tttgcgtgac gacccgcgct
    14941 tgcgtttaac gctgcgtcct gggatgaacg tcgcctatct ggcatttaac accgccaaac
    15001 cgccgctaaa taatcccgct gtccgccatg cactggcact ggcgattaat aaccagcgcc
    15061 tgatgcaatc catctattat ggtacggctg aaacggcggc ctctatttta ccgcgcgcct
    15121 cgtgggccta tgacaacgag gctaaaatta ctgaatacaa tccggcgaaa tcgcgcgaac
    15181 agttgaaggc gttggggctg gaaaacttaa cgctgaaact gtgggtgccc acacgttcgc
    15241 aggcgtggaa ccccagtcca ctgaaaactg ccgaactgat tcaggcggat atggcgcagg
    15301 ttggcgtaaa agtggtgatt gtgccggtag aaggtcgctt tcaggaggcg cggttgatgg
    15361 atatgagcca tgatctgacg ttatccggtt gggcgacgga cagtaacgac ccggacagtt
    15421 tcttccgtcc gttactgagc tgcgcggcaa ttcattcaca gaccaacctc gcccactggt
    15481 gcaatccgaa attcgacagc gtgttgcgta aggcgctctc ctcgcagcag ctggcagcgc
    15541 gtattgaagc ctatgacgaa gcgcagagta ttctggcgca ggaattacct attctgccgc
    15601 tggcgtcgtc attgcgtttg caggcctatc ggtacgatat caaaggtctg gtacttagcc
    15661 cgtttggtaa cgcctccttt gctggggtgt atcgcgagaa acaggatgag gtgaaaaaac
    15721 catgattatc ttcaccttac gccgcatttt gttattgatt gtcaccttgt tcctgctgac
    15781 cttcgttggc ttcagcttga gctatttcac tccacacgcg ccgttacaag gcgcgtcact
    15841 gtggaatgcc tgggtgttct ggtttaatgg cctgatccac tgggattttg gtgtttccag
    15901 cattaacggt caaccaattg ccgaacagtt aaaagagctc ttcccggcga cgatagaact
    15961 gtgcatcctc gccttcggtt ttgcgctgat tgttggcatc cccgtgggga tgattgccgg
    16021 gattacgcgc cataagtggc aggataactt gatcaacgcc atcgccttac tgggcttttc
    16081 aatcccggtg ttctggctgg cgcttctgtt gacactgttt tgttcactca cgctaggttg
    16141 gttgcccgtt tcagggcgtt tcgatctgct ctacgaagtg aaaccgatta ccggttttgc
    16201 gttgattgat gcctggcttt cggactcacc atggcgggat gaaatgatca tgagcgcaat
    16261 ccgccatatg atattgcccg tgatcaccct gtcggttgcg ccaacaactg aagtgatccg
    16321 cctgatgcgt atcagcacta ttgaagtgta tgaccagaac tatgtaaaag cggcggcgac
    16381 ccgcggtttg tcgcgcttta ctattttgcg tcgccacgtt ctgcataacg cgttaccgcc
    16441 ggttattcct cgcctgggct tacagttttc caccatgctg acgctggcga tgatcaccga
    16501 aatggttttt agctggccgg gtttagggcg ctggttaatt aacgccatcc gccagcagga
    16561 ttacgcagcc atttccgccg gagtgatggt gtgtggctca ctggtcatta ttgttaacgt
    16621 gatttctgat attttgggtg ccatggctaa ccctctgaaa cataaggaat ggtatgcctt
    16681 acgatagcgt atacagcgaa aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat
    16741 tttatagtga cgcctctgcg atggtcggcc tgtacggctg cgcgggactg gctgtactgt
    16801 gtatttttgg cggctggttt gcgccttacg gcatcgacca gcaatttctc ggttatcaat
    16861 tattgccgcc atcatggtcg cgctatggcg aagtttcttt cttcctgggg actgacgacc
    16921 tcggacgtga tgttttaagc cgtttactga gcggagctgc gccaaccgtg ggtggcgcat
    16981 ttgtggtgac gcttgccgcg acgatctgtg gcctggtact gggcaccttc gccggggcga
    17041 cacacggcct ccgctcagcg gtgcttaacc atattctgga taccttactg gcgatccctt
    17101 cgctgctgtt ggcaattatc gttgtagcgt ttgccggacc gagtttgtcc cacgctatgt
    17161 ttgctgtctg gctggcattg ctgccgcgta tggtacgttc gatttacagc atggtgcatg
    17221 acgaactgga aaaagagtac gttatcgccg cccgtctgga tggcgcatca acgctgaata
    17281 ttctctggtt tgctgtgatg ccaaacatca ccgccgggct ggtcaccgag atcacccgcg
    17341 cactgtcgat ggcaattctc gatatcgccg cgctgggctt tctcgatctc ggcgcacaac
    17401 tcccctcgcc tgaatgggga gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc
    17461 cgtggactgt catgctgcca ggtgcggcaa ttatgattag cgtgttgttg gttaacctcc
    17521 ttggcgatgg cgttcgtcgt gcgattattg cgggggtgga ataatgccat tactcgatat
    17581 tcgtaacctg accattgaat ttaaaaccgg tgatgagtgg gttaaagccg tcgaccgggt
    17641 aagtatgacc ttaaccgaag gtgaaatccg cggtcttgtt ggtgaatccg gttccggcaa
    17701 gagtttgatt gcgaaagcga tttgtggggt gaataaagat aactggcgtg ttactgctga
    17761 ccgtatgcgt tttgatgata tcgatttgct gcgtctctcc gcacgcgaac ggcgcaaact
    17821 ggttggccat aacgtgtcga tgattttcca ggaaccgcag tcgtgtcttg acccttcaga
    17881 acgtgtgggt cgccagttga tgcaaaacat cccagcctgg acctataaag gccgttggtg
    17941 gcagcgtttt ggctggcgca aacgccgtgc cattgaactg ctgcaccgcg tggggattaa
    18001 agatcacaaa gatgcgatgc gcagttttcc ctatgagttg accgaaggtg aatgtcagaa
    18061 agtgatgata gccattgcac tggcgaatca accgcgtctg ctgattgctg acgaaccgac
    18121 aaactcaatg gagccaacaa cccaggcgca aatctttcgc ctgctgacgc gtctcaacca
    18181 aaacagcaat accactattt tgcttatcag ccatgactta caaatgctta gccaatgggc
    18241 ggataaaatt aacgtgcttt actgcggtca aacagtggaa accgcgccga gtaaggagtt
    18301 ggtgacgatg ccacatcatc cttataccca ggcgctgatc cgcgcgatac cagacttcgg
    18361 cagcgcgatg ccgcataaaa gtcgcctcaa tactctaccc ggcgctatcc cattgctgga
    18421 acagttaccg attggttgtc gtctggggcc acgttgcccg tatgcacaac gagaatgcat
    18481 tgtgacgcca cgtttgacgg gggcgaaaaa tcatctctat gcctgtcatt tcccgctgaa
    18541 catggagaaa gagtgagatg atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt
    18601 tccgctaccg gaccggttgg tttcgtcgtc agaccgtaga agcggtaaaa cccttgagct
    18661 ttacgctacg tgaaggccag acactggcga ttattggcga gaatggttcg ggtaaatcca
    18721 cactggcaaa aatgctggcg ggaatgatag aacctaccag cggcgagtta ttgattgacg
    18781 atcatccgct gcattttggc gattattcct tccgtagtca gcgtattcgc atgatttttc
    18841 aggatccttc gacctcgttg aatccccgtc aacgtatctc acaaattctg gattttccac
    18901 tgcgcctgaa caccgacctg gaaccggaac agcgtcgcaa acagattatt gaaacgatgc
    18961 gtatggttgg gctgctaccg gatcacgtca gttactatcc gcatatgttg gcacccggac
    19021 aaaaacagcg tctgggtctg gcgcgcgcgc tgatattgcg cccaaaagtc attattgccg
    19081 atgaagccct cgcttcactg gatatgtcaa tgcgttcgca gttgattaat ctgatgctgg
    19141 agttacagga aaaacagggc atttcgtata tttatgttac ccagcatatc ggaatgatga
    19201 agcacattag cgaccaggtg ctggtgatgc atcagggcga ggttgtcgag cgaggcagca
    19261 ccgcggatgt gctggcatcg ccgctgcatg aactcaccaa acggctgatt gccgggcact
    19321 ttggcgaagc attgacagcg gatgcgtggc gtaaagatcg ctaattttct gaatcctcct
    19381 tccagattat tcacctcatt ctgacaagag attaaatctt gtcagaatga ggtgatggat
    19441 aaaataaaat caaatgcgcg tgatttaaga cgcaatctca ctttgcagga acgaaagctc
    19501 tggcgatatc ttcgcagccg acgttttggt gatttcaaat ttcgccgtca acatccggtg
    19561 gggagctaca ttctcgattt tgcttgctgc tcggcgcgcg tagtcgttga gctggatggt
    19621 gggcagcatg atttggcagt tgcctatgat accaggcgca ctagctggct tgagtcgcag
    19681 ggctggaccg tgctgcgttt ctggaataac gagattgatt gtaatgagga ggcggtgctg
    19741 gagattattc tgcaggaact gaaccgccgg tcaccctcac cctgaaaggg cgagggggcc
    19801 gaccgagccg aatagctgtt gtggtgaaaa catggagaca gtgctggaga atattcggca
    19861 aggtctgaac catcccagcc atcgccataa aagggttagg ggctgtatga gcctgtttgt
    19921 tgctggggta acaatatttg cacaatatgg tcccctcgcc cctctgggga gagggttagg
    19981 gtaaggggaa aagcgccccc cctgccgcag cctgctccgg tcggacctgg taactatagc
    20041 tactcacagc caggttgatt ataataaccg tttatctgtt cgtactgttt actaaaacga
    20101 cgaatcgcct gattttcagg cacaacaagc atcaacaata aggattaaag ctatgggttt
    20161 tctttccggt aagcgcattc tggtaaccgg tgttgccagc aaactgtcca tcgcctacgg
    20221 tatcgctcag gcgatgcacc gcgaaggagc tgaactggca ttcacctacc agaacgacaa
    20281 actgaaaggc cgcgtagaag aatttgccgc tcaattgggt tctgacatcg ttctgcagtg
    20341 cgatgttgca gaagatacca gcatcgacac catgttcgct gaactgggga aagtttggcc
    20401 gaaatttgac ggtttcgtac actctattgg ttttgcacct ggcgatcagc tggatggtga
    20461 ctatgttaac gccgttaccc gtgaaggctt caaaattgcc cacgacatca gctcctacag
    20521 cttcgttgca atggcaaaag cttgccgctc catgctgaat ccgggttctg ccctgctgac
    20581 cctttcctac cttggcgctg agcgcgctat cccgaactac aacgttatgg gtctggcaaa
    20641 agcgtctctg gaagcgaacg tgcgctatat ggcgaacgcg atgggtccgg aaggtgtgcg
    20701 tgttaacgcc atctctgctg gtccgatccg tactctggcg gcctccggta tcaaagactt
    20761 ccgcaaaatg ctggctcatt gcgaagccgt taccccgatt cgccgtacag ttactattga
    20821 agatgtgggt aactctgcgg cattcctgtg ctccgatctc tctgccggta tctccggtga
    20881 agtagtccac gttgacggcg gtttcagcat cgctgcaatg aacgaactcg aactgaaata
    20941 atcgttctgt tggtaaagat gggcggcgtt ctgccgcccg ttatctctgt tatacctttc
    21001 tgatatttgt tatcgccgat ccgtctttct ccccttcccg ccttgcgtca ggataacgat
    21061 ttcctttacg accaaggagc gcccatggaa caacgccaca tcaccggcaa aagccactgg
    21121 tatcatgaaa cgcaatccag tactacggag tatgacgttc tgcctctggt cccggaagcc
    21181 gcaaaggtca gcgatccctt tctgctcgac gtgatccttg atgaagagac gctggccccc
    21241 ttcctttcat ggctggtccc tgcgcgcgtt cttgcagtgg agttgttccc tgaccagctt
    21301 accgtgaccc gttcacaaac cttcaccgct tatgaacgct tgtcgacggc cctgacggtt
    21361 gctcaggttt gcggcgtcca gcggttatgt aactactatt cggcgcgact tacgccgctc
    21421 cccggacctg attcctccag ggaaagtaat catcggttgg cacaaatcac gcaatatgcc
    21481 cgccaactgg ctagctcgcc ttctattatc gacaaccgat cgcgccagca tctgaatgac
    21541 gtcggtctta ctgtctggga ctgtgtgatc attaaccaaa tcattggttt tattggcttt
    21601 caggcgcgga caattgcgac atttcaggct tatctcgggc atccggtacg ctggttaccc
    21661 gggctggcga tacaaaacta cgccgacgcg tcactgtttg ctgatgaatc aatacgctgg
    21721 cgaagcagct atgaggtgga aaaactacct gaagagcaca caaaaagttc aactgcagaa
    21781 ctttgccaac tggccgaaac actctctctc caccctattt cactttccct tctcgaaaag
    21841 ttgttaaaca gcacacgggt caatgcacag ccggataatc agcttgcggc gttgttatgc
    21901 gcgcgtataa atggcagtcc tgcttgtttt tccacctgta tggattcatc aaatgaatat
    21961 aaaaaaatca gcacccttct gcgcaagggc gaaaatgaaa ttaaccgatg ggctgaccgt
    22021 cattctgttg agcgcgctac cgttcaggcg atacaatggc tgacccgagc acccgatcgc
    22081 tttagcgccg cccagttcag ccctttactc gaacacgaaa aatcatcaac gcagattatt
    22141 aatctgctgg tatggagcgg gctgtgtggc tggataaatc gcttaaaaat cgcgttgggt
    22201 gagacatatt aaccttgccg cgtcagacag attcgcgtaa aactgtcagc cgctctaatg
    22261 gccaccaaaa tagacaatta tgtttcagga caacccgctg ctagcgcagc ttaaacagca
    22321 actgcattcc cagacgccac gcgctgaagg ggtggtaaaa gccacagaaa aaggctttgg
    22381 cttcctggaa gtcgacgcac aaaaaagtta tttcattccg ccgccgcaga tgaaaaaagt
    22441 catgcatggc gaccgaatta tcgcggtgat ccacagtgaa aaagaacgtg aatccgcaga
    22501 gccagaagaa ctggttgaac cgttcctgac tcgtttcgtg ggtaaagttc agggcaaaaa
    22561 tgaccgtctg accatcgttc ctgatcatcc acttttaaaa gacgccattc cttgccgcgc
    22621 agcccgtggc ctgaaccacg agtttaaaga aggcgactgg gcggttgccg aaatgcgccg
    22681 tcatccgctg aaaggcgatc gttctttcta tgctgaactg acacaataca tcacttttgg
    22741 tgacgaccac tttgtaccgt ggtgggttac ccttgcgcgc cataatctgg aaaaagaagc
    22801 accagacggc gtcgctaccg aaatgctcga tgaaggtctg gttcgtgaag atctgacctc
    22861 gctggatttt gtcaccatcg acagtgccag cactgaagat atggatgacg cccttttcgc
    22921 taaggcgctg ccggatgaca aacttcagct gattgtggcg attgccgatc caaccgcgtg
    22981 gattgctgaa ggcagcaagc tggacaaagc cgcgaaaatt cgcgcattca ccaactatct
    23041 gcctggcttc aacatcccta tgctgcctcg cgagctttct gacgatctct gctcactgcg
    23101 cgccaatgaa gtccgcccgg tactggcatg ccgcatgacg ctctccgctg atggcaccat
    23161 tgaagataat atcgaattct ttgccgccac catcgaatcc aaagcgaagc tggtgtatga
    23221 ccaggtttct gactggctgg aaaataccgg tgactggaag cctgaaagtg aagcaattgc
    23281 cgaacaagtc cgtttgctag cgcaaatttg ccaacgccgc ggcgagtggc gtcataacca
    23341 cgcactggtg tttaaagatc gcccggatta ccgctttatt ctcggtgaaa aaggtgaagt
    23401 gctggatatc gtcgccgagc ctcgtcgcat tgccaaccgt atcgtcgaag aagcgatgat
    23461 tgccgctaac atttgtgcgg cccgcgtact gcgcgataag ctcggttttg gcatctataa
    23521 cgtgcatatg ggctttgatc cggcgaatgc cgacgcgctg gcagcgttgc tgaaaacgca
    23581 cggtctgcat gtcgatgccg aagaagtgct cacgctggac ggtttctgca aactgcgtcg
    23641 tgaactggat gcgcaaccaa ctggtttcct cgacagccgc attcgtcgct tccagtcatt
    23701 tgctgaaatt agctctgaac ccggtcctca ctttggcctc ggtctggaag catacgccac
    23761 ctggacttcg ccgatccgta aatatggcga catgatcaac caccgtctgc tgaaagcggt
    23821 tatcaaaggc gaaactgcga cgcgtccgca ggatgaaatc actgtccaaa tggccgaacg
    23881 tcgccgtctc aaccggatgg cagaacgtga tgttggcgac tggttatacg cacgcttcct
    23941 gaaagataaa gccgggaccg gcacccgttt cgcggcggaa attgtcgata tcagccgtgg
    24001 cggcatgcgt gtgcgtttgg ttgataacgg cgctatcgcc tttattccgg cacctttctt
    24061 acacgctgtg cgcgatgaac tggtttgcag ccaggaaaac ggcactgtac aaattaaagg
    24121 tgaaacggtt tacaaagtaa ctgacgttat tgacgtcacc attgccgaag tccgcatgga
    24181 aacccgcagc attattgcgc gcccggtcgc gtaatctcct ttcacggccc attcctcatg
    24241 gatgggccgt ttatttcccc gctctacctt cgttatatcc aggcagattt tttaactgac
    24301 tttcgtttga aaactggcgt ttttccaaaa ctggattaca aataaatata taaaaaaaca
    24361 atgcgtttag ccgccgttaa tttcatgtaa gtcatgttgt tccttttatt taaccgcgga
    24421 ctccgctgtt aaccggagga tatgcatcat gaaaaccgtt agggagtcca caacgttgta
    24481 caactttctc ggatcgcaca atccatactg gcggttgacg gaaagcagcg atgctttgcg
    24541 cttttctacc accaaaacca cagaacctga tcgtacattg cagttatctg ccgaacaggc
    24601 tgctcgcatc agggaaatga cggtcatcac ctccagcctg atgatgagtc tgaccgtcga
    24661 tgaaagcgat ctttctgtgc atctggtagg acgaaaaatc aataaacggg aatgggctgg
    24721 caacgcgtct gcatggcatg acacaccggc ggttgctcgt gatttatcac acgggctttc
    24781 ctttgctgag caggtagttt ctgaagcaca ttccgcaata gtgattctcg acagccgggg
    24841 gaatatccaa cgcttcaatc ggttatgtga agattacaca gggttgaaag aacacgacgt
    24901 cattgggtaa agcgtgttta aactgtttat gagccgtcgt gaagctgcgg catccaggcg
    24961 caataaccgt gtattttttc gaagcggcaa tgcatatgaa gtcgaactgt ggataccaac
    25021 acgtaaaggc cagcggctgt ttctgtttcg caataaattt gtccacagcg gcagtggcaa
    25081 aaacgagatt tttttaatct gttccggtac cgacattacc gaagagcgcc gcactcagga
    25141 gcgactgcgt attctggcaa ataccgacag tatcaccgga ctgccgaatc gtaacgcaat
    25201 gcaggagtta atcgatcacg ctattaatca ggcagataac aataaagttg gggttgtgta
    25261 tcttgatttg gataatttta aaaaggtcaa cgacgcctat gggcatttgt ttggtgacca
    25321 gttattacgc gacgtgtcat tggctatttt aagctgtctc gaacatgacc aggtgttggc
    25381 gcgtccaggt ggcgatgagt ttctggtact ggcatccaat acctcacaaa gcgcgctgga
    25441 agcaatggca tcacgaattt tgacccgctt acgcctcccc tttcgtattg gtttaattga
    25501 agtttatacc agctgttcag taggtatttc gctctctccc gaacatggtt cggacagcgc
    25561 gactattatt cgtcacgccg acacagcaat gtacacagcg aaggaaggcg gacgaggaca
    25621 attttgcgtt tttactccag aaatgaatca gcgggtattt gaatatctct ggctggatac
    25681 caacttgcgt aaagcactgg aaaacgatca gttggttatc gaaaatcacc tagcgtgtcg
    25741 aagtgcgcag tctggaagca ctagtacgtt ggcagtcacc tgaacgtggg ttgattccac
    25801 cgttggactt catttcctac gccgaagagt cagggctaat tgtgccttta ggccgttggg
    25861 tgattctcga tgtcgtacgc caggtggcaa agtggcggga taaaggcata aacctgcgag
    25921 tggcggtaaa tatttctgca cgtcagctcg ccgatcaaac cattttcacc gccctgaaac
    25981 aggttctcca ggaactcaat tttgaatact gccctataga tgttgaactg acagagagtt
    26041 gtctgattga gaatgatgaa ctggcactgt ctgttattca acaatttagc caactaggtg
    26101 cgcaagtgca tctggatgat tttggtaccg gctactcttc actttcgcaa ctggcgcgct
    26161 ttccgatcga tgccatcaaa cttgaccagg tttttgttcg agatattcac aaacaacctg
    26221 tctcgcagtc actggtccgg gcgatcgtcg ctgtggccca ggcattgaat cttcaggtga
    26281 tcgccgaagg tgtagagagt gcaaaggaag atgctttttt aaccaagaac gggatcaatg
    26341 agcggcaagg atttttgttt gccaaaccga tgcccgccgt cgccttcgaa cgctggtata
    26401 aacgctatct gaagcgcgca taagtagatg aatatatata cttagcgcta tatcccgtca
    26461 ttatatatcg tattcctgga tgataattag ccgttgctgt taaacacttc tcttttagcg
    26521 gcaatttttg cattattcat tttccccctt ttgctataag gatgtccttg atgtctgaat
    26581 ttgatgccca gcgcgttgcc gaacgtattg atattgtgtt agatatcctt gtggcaggcg
    26641 attaccactc tgcgatccat aatctcgaga tacttaaagc agaactcttg cgccaggttg
    26701 ctgaatctac gccagacatc ccgaagacac cgtgggagat ttaacaatcg tgccttcagg
    26761 ttttcctatt tgaggatgcc cggcgcgtca gtattttttt atttagtatt ataacgttat
    26821 aagaattaca gcgatgaatt cccgacaaca aactattcta cagatggtca ttgaccaggg
    26881 tcaggttagc gtaaccgatc tggcaaaagc cactggagtt tctgaagtta ccattcgcca
    26941 ggatctcaac accctcgaaa aactgagtta cctccgccgt gcacatggct ttgcagtttc
    27001 gcttgatagt gatgacgtcg aaacccgtat gatgagcaac tatacgctga agcgtgaact
    27061 cgccgagttt gccgcgtcac tggttcaacc gggcgaaacc atctttatcg aaaatggcag
    27121 cagcaatgcc ctgctcgccc ggactctggg ggagcagaag aaaaatgtca ctatcatcac
    27181 ggtcagcagc tacatcgcgc atttgctgaa agacgcgcct tgtgaagtca ttttgctcgg
    27241 tggcgtgtac cagaaaaaaa gcgaaagtat ggttggccct ttgacacgcc agtgcatcca
    27301 gcaggtacat ttcagcaaag catttattgg tattgatggc tggcaacctg aaactggatt
    27361 caccggtcgc gatatgatgc gcaccgatgt ggtcaatgcc gtgctggaaa aagagtgcga
    27421 agcgatagtc ctgactgaca gctcgaaatt tggtgctgta cattcatact ccatcggtcc
    27481 cgttgagcga ttcaatcgcg tgattaccga ttcgaaaata cgcgccagcg atctgatgca
    27541 tcttgagcac agtaaactca ccgttcacgt cgttgacatt taaccatttc ccttctctac
    27601 ggatgatttg cagtttggca aatcatccgc tctaagatga ttcctggttg ataattaaga
    27661 ctatttacct gttattaaca ctctcaagat ataaaattat tatcagcgat ataacaggaa
    27721 gtaattatca cctgcgtgat ataaccctgc gcgcgagcag atttcacgga ataatttcac
    27781 caggcttatt cttagctatt atagttatag agagcttact tccgtgaatc ataaattcag
    27841 gagagagtat tatgtttgta acgagcaaaa aaatgaccgc ggctgttctg gcaattactt
    27901 tggcaatgtc tctgagtgcc tgttctaact ggtctaaacg ggaccgcaac accgcaatcg
    27961 gcgcgggtgc aggggcatta ggcggtgcag tactgaccga tggcagtacg ttgggtacat
    28021 taggtggtgc agctgtcggt ggtgttattg gtcaccaggt cggtaaataa tcgtaaataa
    28081 atgtcgatta tcgactcgca attataatac gcttgattct gttcagtacg aaataactga
    28141 ataatacaca cagtaaaata ttcgaaagcc acggatatat ccgtggcttt ttatgttaac
    28201 cgcctgcgag ttttaccttc atccctttcg cttcaagtag tgattttaat aaatcacgct
    28261 tatcgccctg gatttcaata actccatctt taactgctcc gccgcagccg cattttttct
    28321 tcagttccgc tgcaagtttt gtcagttcgg catcatcgag atcgataccg gtaatcaggc
    28381 aaacgccctt acctttacgt ccactggtct gacgctgaat acgcaccgct ccatcgcctt
    28441 tagggcgtac tggggccgct ttgggctcat caatacgccc ggtttccgtt gagtagacca
    28501 gacggctgtt ggaatcactc atcatgcact ccgctgtaaa gaggcgttga tcgctttcag
    28561 tgtctgcgct ggatctaccg attgcgttac cgggcgacca atcaccatat aatcaacacc
    28621 agccgccaac gcctgttctg gcgtcataat gcggcgctgg tcaccagctt cactcccctg
    28681 cggacgaatg cccggtgtaa ccagtttgaa ctcctgaccg aatacctgtt taaagcgcac
    28741 agcttcctga gcagaacaca ccacaccatc aaggtcacat ttttgcgtca gtgctgccag
    28801 acgttctgca taatctgcag gtgacagtgt cacgccaaga tcggccaggt cgctggcttc
    28861 catgctggtc aacactgtca cagcaatcaa aagcggtgca tctttgccaa acggaaccag
    28921 tgcctcacgc gctgcggtca tcatacgcgc cccaccggag gcatgaacat tcaccatcca
    28981 cacgcctaag tcagctgcag cagcgacagc gtgcgctgca gtgttgggga tatcgtggaa
    29041 tttcaggtca agaaagatat caaaaccacg ctgttgaagt tcgcgcacaa actgtggccc
    29101 aaacaatgta aacatctctt taccgacctt cagacgacaa tcgcgtgggt cgatcttgtc
    29161 gacaaaggac agcgcgtcat cacaattatg ataatcaagg gcaacaacca caggagaatt
    29221 cgtaacggcg cgggaagaag atgaagcagt taacgtcatg accagacctt cttgatgatg
    29281 ggcaccggga gtggcgcgaa acaggtaaac tgcgagcatt ctacccgtga aaataaaaaa
    29341 ttaacagttg cgatttttcc cctagcaggc tttttctgct ccgtcgttgc cggagcacaa
    29401 tgggacatca ataactagta tgttgtaact aaagtcggat ttttttaaaa attacaggcc
    29461 atcaagaccg cgaatcggtt taatagttga ccaggcacga caagacggac aatgccagta
    29521 gagggtgtat gcggtaaaac cacatttctg gcagctataa cgaggcttac tacgcacctt
    29581 ctcgccaacc atgtcacgca gcaccatcag actctctttg gcgcgccctt cttccgcttc
    29641 atttaagtgg taatccatta gcttatggaa tacacgcatg gtcggatgac gctgaagctg
    29701 gcgtgtaata tagacctgtg cagcctcact accgtcgcgc gcttcgatga tatcagcaag
    29761 catcaattca gcatcggcac cggtgttctc ttccaccgcg cgttgcagga attctgccca
    29821 ttcggcagtt ttacccaact gctgatagca ggtttgcaac atttccagcg tttcgctgac
    29881 cagttctctg tcctgggata tgacgcgttg caaactttcg acggctttgg cgtattctcc
    29941 tttcgccata aacacgcgtc ccatcatgat ggatacgcgg gcgctgtttt tatctgccgc
    30001 cgcccctttt tttagcaagg tcatggcacg atcgagatcg tcgctggcca tatgctgcag
    30061 ggctaactca cagtagaaat gggcaatttc gacgcgctgt ttatctttac ccagcttcac
    30121 caggcgttcg gcaacatcaa ttgctttctg ccactcactg gtagcctggt agatttgtag
    30181 caactgttgc agcgcgccaa tgcggaagtc agtttcatcg gtcagctgat tgaacatgtc
    30241 ttccgcgcgg tcgtataacc cggcggccat gtaatcacgc cccagttgtt gaatcgccaa
    30301 cagacgctgt tcataggtca gcgaggcgct ttccattagg gtctgatgga tgcgaatagc
    30361 gcgatcaact tcgccacgcg aacggaacag gtttccgagc gtaaggtggg cttcaacggt
    30421 gcctgtatcc tctttaagca tatcgagaaa caggtctacc gctttatcct gttgattact
    30481 aagcaggaag ttaacccccg ctacgtaatc acgcgacaag cggttggctt catcttgctt
    30541 gttttgttgc gcacttctgc ggcccatata ccagccatag gcagcggcta caggcaaaag
    30601 cagaaacaac aactccagca tagaaagtta ttccttcgcc gctgacgagt gcggcactac
    30661 agccacgtca gtcgcgggtg aaagctggtt ttccagtcgc tttattttac gttcagcgcg
    30721 cgccagggaa acacgaactc gcagccagaa caggccacaa atcaaccaac cgatagcaaa
    30781 ccccgcagca aacaataccg ccagcaatgt ggaaatacgg tactcccctt gcgctaacag
    30841 ataattaaac gtcacctgtt gatcgttctg cgcacccaac gtgaccgaaa tcacgaagat
    30901 cgctaacacc agtaagaaaa tgagtaaata tttcacatta cttcccgtta tgtggttaga
    30961 gcgaataaat tatggtcaac ttaccgcaat agccctttaa agtaccattt tcgcgccgtc
    31021 tgcgaaatgg catccacgcg cattgttgat gatttaaggc ctaaattcgg aaaatcaacc
    31081 agcgttaact ttcttgttct cgttgtgcaa tttcgcgatt ttcttccgca ggtggtgtta
    31141 atggcccaca aattcgttgc gcaagccacg ttgccaccgc caccagcgcc cacgaaatca
    31201 acgtagctac caccagatcg cgtggccaat gcatcccgag cagcaggcgg cttcccatga
    31261 ctcccgttgc ccagaccaac aagatagcaa tggttaacgt tcgccgacgc ggccacagca
    31321 aaccaacggc cagcagtgcc caactggcag caaacatcgt gtgaccggaa ggaaaggcaa
    31381 accccgtctc tttctgccag tgtgaacgca aatattgtgg aatatttttc tcttcagcca
    31441 actgttcttt tactagattt ccgcgttctg ctcgctttaa agtgtagaac tcatcaaccg
    31501 gaatatgatg tgttttttcc aaccagataa caaaaggtcg tggttcctgt actttgtctt
    31561 tgatccagga tttaacgcct tgtccaacaa ggattgccgc cgccagaatg gcaaataata
    31621 caaaggcagc cttaatgcga aaacgcagac accagagaaa ccagccgaac aaaatcaaat
    31681 gtgtaatgac gccccagggc tgggtgacag tttcagtaac ccaaaaagcc gcttttagta
    31741 gccaactttg ttctccaggt tgccaacgcc agccagaaat ccatacggct actggcatga
    31801 caagcaatag tgcagctccc actgcggtac gtcgggcaat cgaacgcatg gcctctcctt
    31861 tttgataagt cccacaatca taactgaaaa tgccagttcc aagaaaaatt gacagatttg
    31921 tgccattccg tgaacgatcg acgcgtcgtg attaggtgaa ccccttctcg ttatggcaaa
    31981 ataagccaat acagaaccag cattatctgg agaatttcat gcagcttaaa cgtgtggcag
    32041 aagccaaact gccaacccca tggggcgatt tcctgatggt gggatttgaa gaactggcaa
    32101 ccggacacga tcatgttgcg ctagtctatg gcgatatttc cgggcatacc ccggtacttg
    32161 cgcgcgtcca ttccgaatgt ctgaccggtg acgccctgtt cagcttgcgc tgcgattgtg
    32221 gcttccagct cgaagcggca ttgacgcaaa ttgccgagga aggccgtggt attttgctgt
    32281 atcaccgtca ggaaggtcgt aacattggtc tgctgaataa aatccgcgct tacgcactgc
    32341 aggatcaagg ttacgatacc gtagaggcta accaccagtt aggcttcgcc gctgatgagc
    32401 gcgacttcac tctttgcgct gatatgttca aactccttgg cgtcaatgaa gtccgcttgt
    32461 taaccaataa cccgaaaaaa gtcgaaattc tgaccgaagc agggattaat attgttgaac
    32521 gcgtaccatt gattgtaggt cgtaacccca ataacgaaca ttatctcgat accaaagccg
    32581 agaaaatggg ccatttgctg aacaaataac cctcttgcat cgtgtaattc atttgcttgc
    32641 cggaagcaaa ataaccggca agcaaatact tgttacttca acatattacg aatgacataa
    32701 tgcaaaatgc cgtcgttctg gtagtaggtc aactccgtcg cggtgtcgat acgacaacgg
    32761 cagggtacga cttcctggct accatccgcg cgcgtaagcg tcaccggaac cgtcgcgcca
    32821 ggttgtagat tttgcagatc gccaatatca atcttctctt ccccggttag ccctaacgtt
    32881 ttacgcgtca cgccttgcgg aaattccagc ggcaggatgc ccatgccaat taaattcgaa
    32941 cggtgaattc gttcaaacga ttcggcaatc accacacgaa ttccaagcag acgaggtcct
    33001 tttgccgccc agtcacggct tgagcctgat ccatactctt tcccggcaat caccgccagc
    33061 ggcgtttgct cctgcttata gcgcatcgca gcatcataaa tagagactac gtcgctgtca
    33121 ggtaaatgcc gcgtcatccc cccttcaacg ccaggcacca tttcattacg gatgcgaata
    33181 ttggcgaagg tgccgcgcat catcacttca tggttaccac gccgcgaacc gtaggagtta
    33241 aagtcttttc gctcaacacc ccgaccttgt agatatcgac ccgctgggct gtcgggctta
    33301 atactgcccg ccggagagat atggtcagtg gtgactgaat cccccagcat tgcgaggatc
    33361 cgcgcaccgt gaatatcttc cactggtgct ggtgttgcct gcatttcatc aaagaaaggc
    33421 gataagcgaa tataggttga gtcctcctgc caaccgtagg tatcggatcg tgtgacgtta
    33481 atttccttcc actctgctgt gccttcaaaa acttctgcgt actctttgcg gaacatttct
    33541 gtggagactt gttctaccgc acgggcaatt tcttgtgccg atggccagat atctttcaga
    33601 taaaccggct cgcctttgcg atcatggccg ataggctcag aagccaggtt gatattcata
    33661 tttcccgcca gcgcataggc aaccaccagc ggtggcgagg ccagccagtt agttttaacc
    33721 agcggatgga tacggccttc aaagttacgg ttgccggaca gcaccgcacc gacggttaaa
    33781 tcgccttttt tgattgccgt ttcgatagga tcgggcagcg gcccagagtt accaatacag
    33841 gtggtacaac cgtatcccac aaggttaaac cccagttcgt cgagatacgg tgtcagtttc
    33901 gcttttgcca gataatcaga aacgactttc gaacccggtg ccaacgacgc tttgacccac
    33961 ggttgccgct tgaggccaag agttacggct tttttcgcca gcaagcctgc ggccatcagc
    34021 acacttgggt tagaggtgtt ggtgcacgag gttatcgcag caatgaccac agcgccatca
    34081 ggtaactgat actgatgtcc gttcataaca taatcgactg gcaggcgatc tttatgcgtg
    34141 gcattcactt tcagttcgtt actggcggca aatgcttttg gtacatcggg cagtgcaacg
    34201 cgatcctgtg ggcgttttgg ccctgccagg ctcgcttcaa cgtcattcat atccagttct
    34261 aacatactgg taaaaatcgg ttcatcgccc gggttacgcc acatgccctg cgctttggca
    34321 tatttttcga ccaactcgac ctgatcttcg ctgcgcccgc ttaaacgcat gtaatcgagg
    34381 gttactgcat cgattgggaa gaagccacag gtggcaccat attctggcga catattggca
    34441 atggtggcgc gatccgccaa cggtagtgaa tccagaccat caccataaaa ttcgacgaat
    34501 ttccccacca cgccatgttt gcgcagcatt tgggtaacag tgagaaccag gtctgtggcg
    34561 gtaatacctt cacgtaattt tccggtaagt ttaaagccca ctacgtccgg gataagcatg
    34621 gaaaccggct ggcctaacat ggcggcttct gcttcgatcc cacccacgcc ccaccccagc
    34681 acgccaaggc cgttgatcat agtggtgtgc gagtcagtac caacgagtgt atccggataa
    34741 gcaatccatt caccgtcctg caattcgctc cacactgctt tgccgagata ttcgaggtta
    34801 acctgatggc aaatgcctgt gcctggcggc acgacgctaa accgactgaa cgcttgcttt
    34861 ccccatttca ggaacacata acgttcgtgg ttgcgctcca tttccaggcg tacgttttct
    34921 tcaaatgcct catcatcacc aaaacgatcg acggtcaccg agtggtcaat gaccaggtcg
    34981 accggtgaga gcgggttaac ctttgcagta tcgccaccga ggcgtttaac cgcttcgcgc
    35041 attgccgcca gatcaacaac ggcaggtacg ccggtaaagt cctgcatcag cacccttgcc
    35101 gggcggtagg caatttcacg gtcagcatgg gcatttttca gccatcccgc cagtgcgtgg
    35161 atatcctctt cggtaactga gttaccatcc tgccagcgca gcaggttttc gagcaaaact
    35221 ttgagtgact tgggtagacg ggtgatatcg cccagtgatt tagcagcaag cggcaggctg
    35281 tagtagtggt aagttttatc tttggcctgc aacgtgtcct tactggcttc tcgtagggtt
    35341 gacgacatag ctcctcctta atgacagggt tgcgaagccc tgattctctt cagggtgaat
    35401 attaaagata acacaaacat cgcgtaacga tttgataaca acccaaattg ataaaagagg
    35461 agaaaccttg tggcaaagca aatgacaacc ccgccgcagc ggggtcaagg atcagtgagt
    35521 aatgatccag aggagttcgg cccagaagag catcgacaga gaaaacgcgc ccatccagga
    35581 ccaatatttc atatttgtgt tgatcatagg ctacaccctt aacggttatt attttgtgtt
    35641 gagataactc aagctgttat tgctgcggtg tattaaaagc cctttaattc attaaaaatg
    35701 ggtaataaga atgatgataa ttattgtttc ggctgatcat cttcagcaaa cagttctata
    35761 aaggcttgct gctgcgatgt tagttgccag tcgtctggaa taacgacgga acattcccgt
    35821 ttcgtttctg gttcattgtc actatgctgg cataatggtg acgactccag ttgctcctga
    35881 ccgaccatag ttatccccac actttccaaa tcagcagtgc gacaaccacc cagaaaagtg
    35941 cagaaccaag aaaaaccgcc agccaggctt tacgttttaa ttcaggatca cggcggggtt
    36001 cctgatttcc agaaggcatt gctaacctca tataatcgcc atcacttatc agcaagacgt
    36061 tgaatgattg gtgtaattaa tcatacgatg agacaaatcc taaagaaact ttagtcaaaa
    36121 atccagcgaa tttacgaatc gtctccagtg aataattcaa tcttttgacc tgaaatagat
    36181 atttgaaaac tggaatttgc gaggcgggta attagacact actttacagg taatttaccc
    36241 tataaatatg agggataaaa atattgtgca cggtatagaa ataagtaaca aaagagaata
    36301 tattccggca ccttcgctac ataaaaggtg ccgaaaataa cgcaagaaat tatttttccg
    36361 gcagttttat atctttaaac atgacctcaa tttcttcatt ggagcgcaat gcgacagccg
    36421 catcaacgac atcacgcgtt aaatgcggtg caaaacgctg aatgaaatca tacatataac
    36481 tgcgcaaaaa agtactacgg cgaaaaccaa ttttggttgt actgtggctg aagatatcgt
    36541 gagcatcaac gcgcacaagg tcgggatcgg cgaccggatc caccgccatg ctggcaatga
    36601 cccctacccc cagccctaac cggacgtaag ttttaatgac gtcagcatcc gttgccgtga
    36661 aaacgatacg cggcgttaac cctgcgcggt taaaggcagt atccagttct gaacgtccgg
    36721 taaagccgaa ggtatatgtc accaacggat attgcgccag ttcttcaatg gtaatggctt
    36781 ttttgcctgc cagcgggtga tccggagtga ctacaatagc ccgattccag tggtagcacg
    36841 gtaacatcac taaatcttca tacagatgca gcgcttctgt ggcgatagcg aaatcggcat
    36901 tgcctttaga gacggcatca gcaatttgtg tcggcgggcc ctggtgcata tgcaaagaaa
    36961 cgcggggata acgctcaata aagcctttga taacgtttgg taacgcgtag cgtgcctggg
    37021 tatgcgtggt ggcgatatac agcgagcctt tatccggcca ggtgtgctca ccggcaaccg
    37081 attttatggc atcgactttc gacaggactt cgcgagcgat acgaattatt tcttgtcctg
    37141 ctggcgttac ctgcgtcagg tgcttgccgc ttcgggaaaa aatttgaatg cctagctcgt
    37201 cttccagcat tctgacttgt ttactgatcc cgggttgtga tgtgtaaagt ccttccgctg
    37261 ttgatgagac attcaggtta tgattgacca cctcaacaat atagcgaagt tgttgtaatt
    37321 tcatgttaaa ccatccactt agacgccatc agaacatcgt tttttcgtca cacctggcgg
    37381 gtgactaata cgatttaata ataaaaaagg tgctaactat aaccactata tcatttatag
    37441 ccttagtgta taggtgtgaa gaaaaaataa tatgcaataa aaaagggccg ctttcgcgac
    37501 cctttgttta taaaaacctg acagaattaa aggttatttt tttccttcaa cccatttgcc
    37561 atcaacataa aatgctgacc agccagtcgc ctttccgtct ttttccgaag agacatattg
    37621 ctgtttggtt ttacggctaa agcgaaccat ggtcttatta ccttccggat cctgctgtgg
    37681 cgcatcggcc agataacgca gtttttccgg cagacggtcg cggaagcgat aaagctcttc
    37741 caccagtggc gcacgcgttt cacgcgattt cgggaaagtg ttggcagcca ggaacacacc
    37801 ggcagcaccg tcacgcagca cgaaataagc atctgatttt tcgcacggca gctcaggtaa
    37861 tggcaccgga tcttctttcg gtggtgccac ttcaccgtta cgtaaaatct tacgtgtgtt
    37921 tttacactct tcgttggtgc aggccatgta tttaccgaaa cgccccattt tcaggtgcat
    37981 ttcagagcca catttttcac actcaacgat cgggccgtca taacctttaa tgcggaattc
    38041 gccctcttcg atctcgtaac cgtcgcaggt tgggttatta ccacagacat gcaacttacg
    38101 tttcggatcg atgagatagc tgtccatcgc cgtgccgcat ttcgggcaac gacgttttgc
    38161 gcgcagcgcg ttggtttcag catcttcgcc ttccagcacg ttcagcactt cgttttccgg
    38221 caccaggtta atggtggttt tgcaacgctc tttcggcggc agcgcatagc cagaacagcc
    38281 aaggaatacc ccggtactcg ctgtgcgaat ccccattttg cgaccacaag tcgggcagtc
    38341 aatgctggtc agaaccatct ggttcgggcg cataccaccc tcttccggat ctttttcagc
    38401 tttatctaac tgctgggtga aatccgagaa gaagtgatcc agtacagctt tccactctgc
    38461 ttcgtgattt gccacctggt cgaggctgtt ttccatctgc gcggtaaagt cgtagttcat
    38521 taactcgcgg aaattttctt caaggcgatc ggtgacgatt tcgcccattt tttccgcata
    38581 gaaacgacga ttttctactc gcacatagcc acgatcctga atggtcgaaa tgatcgacgc
    38641 ataggtagac ggacgaccga taccacgttt ttccagttct ttaaccagcg atgcttcact
    38701 gaaacgggct ggcggcttgg taaagtgctg ggctggtgtt agttcaacga gcgtcagagc
    38761 atcgccttta tcgactgccg gtaagatacg atcttcatcg cctttacgca gtgcaggcat
    38821 cacttttgtc cagccatcaa agcgcaaaat acgaccgcgt gctttcaggc ggaaatcgcc
    38881 cgcaccaacg gtcagcgtcg tggagtcata tttcgctggg gtcatctggc aggcaacgaa
    38941 ctgacgccag attaactggt acagtttctg cgcatctgct tccatatcct tcagcgattc
    39001 cgccatcaca ttgacgtcag aaggacgaat cgcttcgtgc gcttcctgtg agttttcttt
    39061 gctggcgtac tgattcggac tttccggcag atatttctta ccaaaattat cgctgatata
    39121 accgcgaacc atatttaccg cgtcctgact caggttagtg gagtcggtac gcatgtaagt
    39181 gatatagcct gcttcataca aacgctgcgc catcatcatg gtttttttca cgccaaaacc
    39241 aagacgggtg ctggcagctt gttgcagcgt agaggtaata aaaggagcgc caggtttact
    39301 ggttgtcggt ttgtcttcac gttccagcac gctgtagcgc gctttttcca gcagacttac
    39361 cgcagcctga gtttgttctt tgttgaccgg acggaacggt ttgtcattct gatgagtcac
    39421 ctgcaactcc aacgcttcac cagatggcgt agtcgtgctg gcatcgactt cccagaactc
    39481 ttccggtacg aacgctttaa tttcacgctc acgctctacc accaggcgga ccgccaccga
    39541 ctgcacacga ccggcagaca gaccacgagc gatctttttc cacagcagcg gcgaaaccat
    39601 ataccccacc acgcggtcca taaagcgacg cgcctgctgg gcattaacac gatcaatatt
    39661 cagctcaccc ggtttgttaa atgcctggcg gatcgcgttt ttagtaattt cgttaaacac
    39721 cactcggcta tagcgcgcat catcaccacc aatcacttcc cgcaggtgcc atgcaatggc
    39781 ttccccttcg cggtcaaggt cggttgcgag atagatgtgg tcggcttttt cagccagttg
    39841 tttcagttca gagacgacct tctctttacc aggcaacact tcatagtgcg cctcccaatt
    39901 gtgccacggg tcaaccccca tacggttgac gagagcgcca cgttcatcct ttttaggctt
    39961 tttagccgtc ttggtggagg tagagtcggc actctttttg gcagctgagc cactggtcgg
    40021 caaatcgcgg atgtgaccga cgctggattt caccacgtag tcacaaccca gatacttgtt
    40081 gatcgttttg gcttttgccg gggactcaac gatgacaaga gctttaccca tattcacctt
    40141 acctaatttg attcttccag gaatgcgtcg cacgttgata aaccttccac tggcaacgag
    40201 ttaccgatat ggactcgctc tctgcggata tcaacccctt gtcgtgactc tgttcacgaa
    40261 aattcaggtg actgagttaa caggtcttgg cctacaggcg ctatagcacg acggattttt
    40321 ggtcgaatgt caagcaaatc tattgccaga ttgacgaaag cgtcacactg tacctgataa
    40381 aatccgatac gcaactttat tagcatgcaa aatccaaagt tgccagtaag gcgctcttgc
    40441 caccagcgtg gtatatttgt ctgcaagcca tgcctggcgt agactaatgt ccttgtttaa
    40501 ggagtaaata atgaacaaag agacacaacc catcgaccgc gaaacgctgt tgaaagaggc
    40561 caacaaaatc attcgtgagc atgaggatac gctcgcaggg attgaagcca ccggcgtcac
    40621 gcagcgtaat ggcgtgctgg tatttaccgg cgactacttt ttagatgaac aaggattgcc
    40681 aacagccaaa agcaccgcgg tgtttaatat gttcaaacac ctggcccatg tgctttcaga
    40741 aaaatatcat ctggttgatt aaagcaaaac gcgaggtctt agcctcgcgt ttgtctttta
    40801 catcaatggc ttttgacccc gctgccacca gcgtagcaac aatcgatcgg cgctctctgc
    40861 cgcgctgccg gtgaatcggt caatgagtcg tttacgctgc atatagcgta cattaaccac
    40921 ttcacggcct tccatcaggc taagaataac ttcatcactg gtgttgattt catcaaccag
    40981 gcctttctct accgcctgtt gtccgtacca gtgttcaccc gttgccacct gttcaatatc
    41041 cagagacgga cgcatacgct tcacaaaatc tttaaataac tgatgcgttt cgttcagctc
    41101 ttcgcggaat ttctcccgcc cttcttcggt attttcaccc agcaaggtca gcgtgcgctt
    41161 atactgcccg gcggtgtgca gttcgatatc aatatctttg cttttcagga agcggttaaa
    41221 gttgggcatt tgcgccacca ccccaatgga acccacaata gcaaacggtg cggaaacaat
    41281 tttgtccgcc acacaggcca tcatgtaacc gccgctggca gcgactttgt ctaccgtaac
    41341 agttaaagga atgtttttat cacgcagacg ctgcagctgc gaagccgcca acccgtaacc
    41401 atgcaccatg ccaccagggc tttccagacg gagcacaacc tgatcctgcg gtttgaatgc
    41461 tgcgagtacc gccgttatct cttcacgtag cgagttcact tcatgggcgt ccatgctgcc
    41521 tttaaaatcc agcacccaga cgcgaggttt actgtcagtt gccacctcgc ccagtttggc
    41581 tttcgctttt gctgctttcg cttcttgctt gtgctttttc ttctgcgctt tgtgccactg
    41641 tttttgctga tgtgagtcca tcagcgccgc ggccagttct tctttcatct ccttatactg
    41701 ttcgctgaga ttgttgaccc gtaactcgcc acgctggcgt ttattacgtt gagcaacatt
    41761 gacaataatg gcggcaatcg ccgcaatcgc tagcacaacg gtaacgattt tagccaaaaa
    41821 caaaccatat tcagacaaca attccacgag tccccacctt ggttaaacaa cgctttggct
    41881 cacagtttac aacaggcatg ttaagccgtc tcgcagagat gttattgcag cgaaagtatg
    41941 acaaagtatc cattttgcgg tgttatcgcc ccaggctgat tgaaatgccc ttctgtttca
    42001 ggcataaagc cccaaagtca taaagtacac tggcagcgcg acagacaacg tcgttgagga
    42061 gtagccatgc attaccagcc aaaacaagat ttactcaatg atcgcattat cctggtgacg
    42121 ggagccagcg atgggattgg tcgtgaagcc gcgatgacgt atgcacgcta tggtgcgaca
    42181 gtgattctgt tgggccgtaa tgaagaaaaa ttacgtcagg tagccagcca cataaacgaa
    42241 gaaactgggc gtcagccaca gtggtttatt ctcgatttgc tgacctgcac gtccgaaaat
    42301 tgccaacaac tggcacagcg cattgtcgtt aattatccgc gtctggatgg tgttttgcat
    42361 aatgccggat tgctcggcga tgtttgccca atgagcgaac aaaatccgca ggtctggcag
    42421 gacgtcatgc agatcaacgt taatgccacc tttatgctca cccaggcact gcttccttta
    42481 ttactcaaat cggacgccgg ttcactggtc tttacttcat caagcgttgg acgtcaggga
    42541 cgagccaact ggggtgcata tgcagcgtcg aaatttgcca ccgaagggat gatgcaggta
    42601 ctggccgatg aatatcagca gcgcctgcgc gtcaactgca ttaacccagg cggtacgcgc
    42661 accgcaatgc gcgcaagcgc cttcccgacc gaagatccac agaaacttaa aacacccgct
    42721 gatatcatgc cgctctacct ctggctgatg ggcgatgaca gccgccgtaa aaccggcatg
    42781 acctttgacg cccaaccggg ccgtaaacca ggaatttccc aatgagtgat gaacgctacc
    42841 aacagcgtca gcagcgagtg aaagaaaaag tagatgctcg tgtggcccag gcccaggatg
    42901 aacgcggtat tatcatcgtc tttaccggca atggaaaagg caaaaccacc gcggcatttg
    42961 gtacggcaac acgcgcagtt ggtcacggaa aaaaagtggg cgtcgtgcag tttattaaag
    43021 gcacctggcc taatggcgaa cgcaatctgc tggagccaca tggcgttgag tttcaggtga
    43081 tggcaacggg ctttacctgg gatacacaaa accgcgagtc tgataccgcc gcctgccgcg
    43141 aagtctggca acatgcaaag cggatgcttg ctgattcctc actggatatg gttttgcttg
    43201 atgaactgac gtatatggtg gcgtatgact atttgccact ggaagaagtg gtgcaggcgt
    43261 taaatgaacg tccacatcaa cagacggtga ttatcacggg tcgaggctgt catcgggata
    43321 ttcttgaact ggcggatacg gtaagtgagc tgcgccccat cagacatgcg tttgatgccg
    43381 gtgttaaggc gcaaataggg attgattatt aatctgtaaa aatgccggat atgacgtccg
    43441 gcatactttg attaaccgtt attacgcccg ccagaacgac gactgccgct cacctgactg
    43501 tgacgtttca ccgcacgacg aatctgatta gccttcatgc gacgacggtc tttttctaca
    43561 gcgactttag agctggtttc tggcggaagc tccaccagtt cgcgcagata gttagtctgg
    43621 gcgaggtcca gctccgtcca gccaccgcgt ggcagacctt ttggcagtgg gatatcaccg
    43681 taacgaacac ggatcaggcg gctcacctgc acaccaaccg cttcccacag acgacgaact
    43741 tcacggttac ggccttcggt cagagtcacg ttgtaccact ggttgatccc ttcgccgccg
    43801 ctgaacttga tggttttaaa agctgccgga ccatcttcca gttgcacgcc acgactcaaa
    43861 tcacgcagtt tcgcgtcgtc aacctgacca aatacacgca cggcgtattc acgttcaact
    43921 tcacggcttg gatgcattaa acggtttgcc agttcaccat cggtggtgaa cagcagcaga
    43981 ccacaggtgt taacgtccag acgacctacg gcaatccagc gtgcgccacg cagctttggc
    44041 agacggtcaa acaccgtcgg acgcccttcc ggatcgttac gggtacacaa ctcaccttcc
    44101 ggtttgtaat aggccagaac gcgacaaatt tgttcagcgg actcacgtac cgaaatcaga
    44161 tgaccatcga tacgaatttt cagcccaggg gtaacttcaa cgcgatcgcc gagtttagca
    44221 attttgccat caacactcac acgaccggct tcaataatag attcgatttc acggcgggag
    44281 ccgtggccag cgcgggccag cactttctgt aatttttcgc tcatagagct tccttttggt
    44341 gtcgccttca caggcgtcga acaggagaat caatggcgcc gtatagcgcc attttaaggc
    44401 cgcgtagtat acagagttac gcacttataa gaaaggcttc acatcaccta cgccttcacg
    44461 caccacgacc ggcgtatcat cggtaagatc aataaccgtt gttggtttct gcccgagata
    44521 gccgccgtga ataatcaaat ctacctgttt ttccagacgg tctttaattt cttcgggatc
    44581 ggattcggta aattcgctgc ctggcagcat tagcgaagtg gaaagcatcg gttcgcccag
    44641 tgcttcaagc aacgcctgcg caatcgggtt agaaggcaca cgcataccga tggttttgcg
    44701 tttctcctgc aacagacggc gtggcacttc cttcgtcccc ttcaggatga aggtatagtt
    44761 gcccggcgtg ttgtttttca tcaaacgaaa cgcaacgtta tccacgaatg aataggtcga
    44821 gagttcagaa agatcgcgac acatcagggt aaagttgtga ccgtccggca gctggcgaat
    44881 acgacaaata cgctccatgg cgtttttatc ttcaatttta cagccgagcg catagccgga
    44941 atcagttgga tagacaatca ccccgccttt acgcacgatc tccaccgcct ggttgatcag
    45001 acgttgctgc gggttatcag gatgaatata aaaaaactgg ctcataattc cctctctgtg
    45061 gtgttctgcg gctgttccca tagctgccag acgccttcaa cgcctgcggg cagccagagt
    45121 ttacgaccca gttcgatcca cgggcatggc tgatgaaaat cagatccttg tgatgcccat
    45181 aaatgatgct gacgcgcaag tgtggccagc tgggtgcgtt cattgggcga ttgctggcac
    45241 tgcgcgactt ccatcgcgtc gccgtggtgt tcggcaaaat gcgcaaccaa tcttttcagc
    45301 catttagcac taagattgta ccgcccagga tgagctagca ccgccttacc gccagaatga
    45361 tgaatgacat caatagcttg ttctattgta caccactgtg gcggaacgta tccggttttc
    45421 ccgcgcgcca gatacttttt aaagacatct gccattgaac tggctttgcc gcactccaca
    45481 aggaaacgcg caaaatgacc gcgcgtcact gcgccgccct gcgccagtcg ttgtgcgcct
    45541 tccagcgccc caggtatttg cgctttttca agtctttcgg caatttgctg cgcccgctga
    45601 ttgcgccgtt ctgtctgctg cgcgaggaac tcacacatta gcggatgagt aatatcaata
    45661 ttcagcccga caatatgaat ttcatgattt tcccagaccg tggaaatttc cacgccggga
    45721 ataagattca gcgccagtcc cgaacgtgaa atttcctctc ttgctggcgc gattgcagct
    45781 gtggtgtcat ggtcggtgat cgccagggtg ccgacgcgca tctcgactgc acggtgcacc
    45841 aatgcttctg gcgtcaggca gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc
    45901 actgcataat tcgtgtcgct caaggcgcac tcccgttctg gataatgttt tttgcgccga
    45961 catcataacg ggtctggcaa atattctgaa atgagctgtt gacaattaat catcgaacca
    46021 gttaactagt acgcaagttc acgtaaaaag ggtatcgaca atgaaagcaa ttttcgtact
    46081 gaaaggttgg tggcgcactt cctgaaacgg gcagtgtatt caccatgcgt aaagcaatca
    46141 gatacccagc ccgcctaatg agcgggcttt tttttgaaca aaattagaga ataacaatgc
    46201 aaacacaaaa accgactctc gaactgctaa cctgcgaagg cgcttatcgc gacaacccga
    46261 ctgcgctttt tcaccagttg tgtgggaatc gtccggcaac gctgctgctg gaatccgcag
    46321 atatcgacag caaagatgat ttaaaaagcc tgctgctgat agacagtgcg ctgcgcatta
    46381 cagcattagg tgacaccgtc acaattcagg cactttccgg caatggcgaa gccctgctgg
    46441 cactactgga taacgccctg cctgcgggtg tggaaagtga acaatcacca aactgccgtg
    46501 tgctgcgctt cccccctgtc agtccactgc tggatgaaga cgcccgctta tgctcccttt
    46561 cggtttttga cgctttccgt ttattgcaga atctgttgaa tgtaccgaag gaagaacgag
    46621 aagcaatgtt cttcggcggc ctgttctctt atgaccttgt ggcgggattt gaagatttac
    46681 cgcaactgtc agcggaaaat aactgccctg atttctgttt ttatctcgct gaaacgctga
    46741 tggtgattga ccatcagaaa aaaagcaccc gtattcaggc cagcctgttt gctccgaatg
    46801 aagaagaaaa acaacgtctc actgctcgcc tgaacgaact acgtcagcaa ctgaccgaag
    46861 ccgcgccgcc gctgccagtg gtttccgtgc cgcatatgcg ttgtgaatgt aatcagagcg
    46921 atgaagagtt cggtggcgta gtgcgtttgt tgcaaaaagc gattcgcgct ggagaaattt
    46981 tccaggtggt gccatctcgc cgtttctctc tgccctgccc gtcaccgctg gcggcctatt
    47041 acgtgctgaa aaagagtaat cccagcccgt acatgttttt tatgcaggat aatgatttca
    47101 ccctatttgg cgcgtcgccg gaaagctcgc tcaagtatga tgccaccagc cgccagattg
    47161 agatctaccc gattgccgga acacgcccac gcggtcgtcg cgccgatggt tcactggaca
    47221 gagatctcga cagccgtatt gaactggaaa tgcgtactga ccataaagag ctgtctgaac
    47281 atctgatgct ggttgatctc gcccgtaatg atctggcacg catttgcacc cctggcagcc
    47341 gctacatcgc cgatctcacc aaagttgacc gttattccta tgtgatgcac ctcgtctctc
    47401 gcgtagtcgg cgaactgcgt cacgatcttg acgccctgca cgcttatcgc gcctgtatga
    47461 atatggggac gttaagcggt gcgccgaaag tacgcgctat gcagttaatt gccgaggcgg
    47521 aaggtcgtcg ccgcggcagc tacggcggcg cggtaggtta tttcaccgcg catggcgatc
    47581 tcgacacctg cattgtgatc cgctcggcgc tggtggaaaa cggtatcgcc accgtgcaag
    47641 cgggtgctgg tgtagtcctt gattctgttc cgcagtcgga agccgacgaa acccgtaaca
    47701 aagcccgcgc tgtactgcgc gctattgcca ccgcgcatca tgcacaggag actttctgat
    47761 ggctgacatt ctgctgctcg ataatatcga ctcttttacg tacaacctgg cagatcagtt
    47821 gcgcagcaat ggtcataacg tggtgattta ccgcaaccat attccggcgc agaccttaat
    47881 tgaacgcctg gcgacgatga gcaatccggt actgatgctt tctcctggcc ccggtgtgcc
    47941 gagcgaagcc ggttgtatgc cggaactcct cacccgcttg cgtggcaagc tgcccattat
    48001 tggcatttgc ctcggacatc aggcaattgt cgaagcttac gggggctatg tcggtcaggc
    48061 gggcgaaatt cttcacggta aagcgtcgag cattgaacat gacggtcagg cgatgtttgc
    48121 cggattaaca aacccgctgc cggtggcgcg ttatcactcg ctggttggca gtaacattcc
    48181 ggccggttta accatcaacg cccattttaa tggcatggtg atggcagtac gtcacgatgc
    48241 ggatcgcgtt tgtggattcc agttccatcc ggaatccatt ctcaccaccc agggcgctcg
    48301 cctgctggaa caaacgctgg cctgggcgca gcagaaacta gagccagcca acacgctgca
    48361 accgattctg gaaaaactgt atcaggcgca gacgcttagc caacaagaaa gccaccagct
    48421 gttttcagcg gtggtgcgtg gcgagctgaa gccggaacaa ctggcggcgg cgctggtgag
    48481 catgaaaatt cgcggtgagc acccgaacga gatcgccggg gcagcaaccg cgctactgga
    48541 aaacgcagcg ccgttcccgc gcccggatta tctgtttgct gatatcgtcg gtactggcgg
    48601 tgacggcagc aacagtatca atatttctac cgccagtgcg tttgtcgccg cggcctgtgg
    48661 gctgaaagtg gcgaaacacg gtaaccgcag cgtctccagt aaatccggct cgtcggatct
    48721 gctggcggca ttcggtatta atcttgatat gaacgccaat aaatcgcgcc aggcgctgga
    48781 cgagttaggc gtatgtttcc tctttgcgcc gaaatatcac accggattcc gccacgcgat
    48841 gccggttcgc cagcaactga aaacccgcac cctgttcaat gtgctggggc cattgattaa
    48901 cccggcgcat ccgccgctgg cgttaattgg tgtttatagt ccggaactgg tgctgccgat
    48961 tgccgaaacc ttgcgcgtgc tggggtatca acgcgcggcg gtggtgcaca gcggcgggat
    49021 ggatgaagtt tcattacacg cgccgacaat cgttgccgag ctgcatgacg gcaaaattaa
    49081 gagctatcaa ttgaccgctg aagattttgg cctgactccc taccaccagg agcaactggc
    49141 aggcggaaca cctgaagaaa accgtgacat tttaacacgc ttgttacaag gtaaaggcga
    49201 cgccgcccat gaagcagccg tcgctgcgaa cgtcgccatg ttaatgcgcc tgcatggcca
    49261 tgaagatctg caagccaatg cgcaaaccgt tcttgaggta ctgcgcagtg gttccgctta
    49321 cgacagagtt accgcgctgg cggcacgagg gtaaatgatg caaaccgttt tagcgaaaat
    49381 cgtcgcagac aaggcgattt gggtagaaac ccgcaaacag cagcaaccgc tggccagttt
    49441 tcagaatgag gttcagccga gcacacgaca tttttatgat gcgctacagg gtgcacgcac
    49501 ggcgtttatt ctggagtgta aaaaagcgtc gccgtcaaaa ggcgtgatcc gtgatgattt
    49561 cgatccggca cgcattgccg ccatttataa acattacgct tcggcaattt cggtgctgac
    49621 tgatgagaaa tattttcagg ggagctttga tttcctcctc atcgtcagcc aaatcgcccc
    49681 gcagccgatt ttatgtaaag acttcattat cgacccttac cagatctatc tggcgcgcta
    49741 ttaccaggcc gatgcctgct tattaatgct ttcagtactg gatgacgacc aatatcgcca
    49801 gcttgcagcc gtcgctcaca gtctggagat gggggtgctg accgaagtca gtaatgaaga
    49861 ggaactggag cgcgccattg cattaggggc aaaggtcgtt ggcatcaaca accgcgatct
    49921 gcgtgatttg tcgattgatc tcaaccgtac ccgcgagctt gcgccgaaac tggggcacaa
    49981 cgtaacggta atcagcgaat ccggcatcaa tacttacgct caggtgcgcg agttaagcca
    50041 cttcgctaac ggttttctga ttggttcggc gttgatggcc catgacgatt tgcacgccgc
    50101 cgtgcgccgg gtgttgctgg gtgagaataa agtatgtggc ctgacgcgtg ggcaagatgc
    50161 taaagcagct tatgacgcgg gcgcgattta cggtgggttg atttttgttg cgacatcacc
    50221 gcgttgcgtc aacgttgaac aggcgcagga agtgatggct gcggcaccgt tgcagtatgt
    50281 tggcgtgttc cgcaatcacg atattgccga tgtggtggac aaagctaagg tgttatcgct
    50341 ggcggcagtg caactgcatg gcaatgaaga tcagctgtat atcgatacgc tgcgtgaagc
    50401 tctgccagca catgttgcca tctggaaagc attaagcgtc ggtgaaaccc tgcccgcccg
    50461 cgagtttcag cacgttgata aatatgtttt agacaacggc cagggtggaa gcgggcaacg
    50521 ttttgactgg tcactattaa atggtcaatc gcttggcaac gttctgctgg cggggggctt
    50581 aggcgcagat aactgcgtgg aagcggcaca aaccggctgc gccggacttg attttaattc
    50641 tgctgtagag tcgcaaccgg gcatcaaaga cgcacgtctt ttggcctcgg ttttccagac
    50701 gctgcgcgca tattaaggaa aggaacaatg acaacattac ttaaccccta ttttggtgag
    50761 tttggcggca tgtacgtgcc acaaatcctg atgcctgctc tgcgccagct ggaagaagct
    50821 tttgtcagcg cgcaaaaaga tcctgaattt caagcccagt tcaacgacct gctgaaaaac
    50881 tatgccgggc gtccaaccgc actgaccaaa tgccagaaca ttacagccgg gacgaacacc
    50941 acgctgtatc tcaagcgtga agatttgctg cacggcggcg cacataaaac caaccaggtg
    51001 ctggggcagg cgttgctggc gaagcggatg ggtaaaaccg aaatcatcgc cgaaaccggg
    51061 gccggtcagc atggcgtggc gtcggccctt gccagcgccc tgctcggcct gaaatgccgt
    51121 atttatatgg gtgccaaaga cgttgaacgc cagtcgccta acgtttttcg tatgcgctta
    51181 atgggtgcgg aagtgatccc ggtacatagc ggttccgcca cgctgaaaga tgcctgtaat
    51241 gaggcgctac gcgactggtc cggcagttat gaaaccgcgc actatatgct gggcaccgcg
    51301 gctggcccgc atccttatcc gaccattgtc cgtgagtttc agcggatgat tggcgaagaa
    51361 accaaagcgc agattctgga aagagaaggt cgcctgccgg atgccgttat cgcctgtgtt
    51421 ggcggcggtt cgaatgccat cggcatgttt gctgatttca tcaatgaaac caacgtcggc
    51481 ctgattggtg tggagcctgg tggtcacggt atcgaaactg gcgagcacgg cgcaccgcta
    51541 aaacatggtc gcgtgggcat ttatttcggt atgaaagcgc cgatgatgca aaccgaagac
    51601 gggcagattg aagaatctta ctccatctcc gccggactgg atttcccgtc tgtcggtcca
    51661 caacacgcgt atcttaacag cactggacgc gctgattacg tgtctattac cgatgatgaa
    51721 gcccttgaag ccttcaaaac gctgtgcctg cacgaaggga tcatcccggc gctggaatcc
    51781 tcccacgccc tggcccatgc gctgaaaatg atgcgcgaaa acccggataa agagcagcta
    51841 ctggtggtta acctttccgg tcgcggcgat aaagacatct tcaccgttca cgatattttg
    51901 aaagcacgag gggaaatctg atggaacgct acgaatctct gtttgcccag ttgaaggagc
    51961 gcaaagaagg cgcattcgtt cctttcgtca cgctcggtga tccgggcatt gagcagtcat
    52021 tgaaaattat cgatacgcta attgaagccg gtgctgacgc gctggagtta ggtatcccct
    52081 tctccgaccc actggcggat ggcccgacca ttcaaaacgc cactctgcgc gcctttgcgg
    52141 caggtgtgac tccggcacaa tgttttgaaa tgctggcact gattcgccag aaacacccga
    52201 ccattcccat tggcctgttg atgtatgcca atctggtgtt taacaaaggc attgatgagt
    52261 tttatgccca gtgcgagaaa gtcggcgtcg attcggtgct ggttgccgat gtgccagttg
    52321 aagagtccgc gcccttccgc caggccgcgt tgcgccataa tgtcgcacct atcttcatct
    52381 gcccgccaaa tgccgatgac gacctgctgc gccagatagc ctcttacggt cgtggttaca
    52441 catatttgct gtcacgagcg ggcgtgaccg gcgcagaaaa ccgcgccgcg ttacccctca
    52501 atcatctggt tgcgaagctg aaagagtaca acgctgcacc tccattgcag gggtttggta
    52561 tttccgcccc ggatcaggta aaagcagcga ttgatgcagg agctgcgggc gcgatttctg
    52621 gttcggccat tgttaaaatc atcgagcaac atattaatga gccagagaaa atgctggcgg
    52681 cactgaaagt ttttgtacaa ccgatgaaag cggcgacgcg cagttaatcc cacagccgcc
    52741 agttccgctg gcggcatttt aactttcttt aatgaagccg gaaaaatcct aaattcattt
    52801 aatatttatc tttttaccgt ttcgcttacc ccggtcgaac gtcaacttac gtcatttttc
    52861 cgcccaacag taatataatc aaacaaatta atcccgcaac ataacaccag taaaatcaat
    52921 aattttctct aagtcacata ttcctcaggt aattgttaat atatccagaa tgttcctcaa
    52981 aatatatttc cctctatctt ctcgttgcgc ttaatttgac taattctcat tagtgactaa
    53041 ttttaatgag tgtcgacaca caacactcat attaatgaaa caatgcaacg caacgggaga
    53101 aataacatgg ccgaacatcg tggtagttca ggaaatttcg ccgaagaccg tgagaaggca
    53161 tccgacgcag gccgtaaagg cggtcagcat agcggcggta attttaaaaa tgatccgcaa
    53221 cgcgcatctg aagcgggtaa aaaaggcggt caacaaagcg gtggtaataa atcaggcaaa
    53281 tcctgattca ccgttaatta ttaatatcag ctgataaaga taattgcatc tgcgggccgt
    53341 atggctcgca gtgatttcac tggagaaaaa tatgaatatg aagaccattg aagatgtatt
    53401 tattcaactg ctttcagata cctacagcgc agaaaaacaa ttaacccggg cactggcaaa
    53461 actcgcaaga gcaacatcaa atgaaaaatt aagtcaggct tttcatgcgc acctcgagga
    53521 aactcatgga cagattgaac gaattgatca agttgtggaa tcagaatcaa atctgaaaat
    53581 taagcgcatg aaatgtgtgg caatggaagg tcttattgaa gaagctaatg aggtcatcga
    53641 aagtaccgag aaaaacgaag tgcgtgatgc cgcactgatt gccgcagcac agaaagtcga
    53701 gcattatgag attgccagtt acgggacatt agcgacgctg gctgaacaat taggttaccg
    53761 taaagcagcg aagcttctga aagaaaccct ggaagaagaa aaggccaccg acatcaaact
    53821 gactgatctg gcccttaata acgtaaataa gaaagccgaa aataaagcct gaaatatgaa
    53881 ttttaacttt tagtcatttt ataaagagga cattttcatg aatcgtattg aacattatca
    53941 tgactggtta cgtgacgccc acgcaatgga aaagcaagcc gaatctatgc ttgaatccat
    54001 ggccagccgt atagataatt atcctgaact acgcgctcgt attgaacaac atcttagtga
    54061 aaccaaaaac cagattgttc aactggaaac tattcttgat cgtaatgaca tttcacgttc
    54121 agtcattaaa gattccatga gtaaaatggc tgcgcttggg cagtcaatcg gtggtatatt
    54181 cccttctgat gaaatagtta aaggctctat tggcggatat gtcttcgagc aatttgaaat
    54241 cacctgttac acctcactat tagcagcagc aaaaaatgcc ggtgatacag tctcaattcc
    54301 gatcatcgaa gcgattttaa atgaggaaaa gcaaatggcc gactggctga ttcagcatat
    54361 tccgcaaaca actgagaaat ttttaattcg ttctgaaact gatggcgtag aagcgaagaa
    54421 ataataccct ttataccatg tccttattga ccccgtatat tacggggtcg tttttgtgcg
    54481 gaattaaaaa cgatatcctg ctgagaacat aaacacccac ggatcgaggc gtacgctatc
    54541 gtgttgctgt gcaccgccta gcttataatt ggcggtggta tcgatatcca tgtaccacac
    54601 tgacatgtta accaaccagt cacggttaat cagataatca acccccacct gcccggcagc
    54661 tccccaggaa tctttcagac tgagatcgga aagccccgcc tctttgccat gatcgttaaa
    54721 tccattatca aagaaggtgg tgtagttaat accggcccca acgtaaggac ggaatttgct
    54781 gctggcatca ccaaaatacc actgcgccat cagtgttggc ggcagatggt gaacgattgc
    54841 aatatcgccg gtcgcccggg tgccgatttt atggcggaac ggcgtcgctg ccagtaattc
    54901 cacaccaatg ttgtcggtcg ccatataagt aaacgtcaag cccagttgag tgttattggt
    54961 cacgctgaat ccacccagac ttcctaacgt accaccagca ccttctgttg gacgtacggt
    55021 tgcagaacct gcacgcataa aaaattcgcc tgcttcatgc gcaaaggcac tgccagaaag
    55081 aagagttgtt actgccaaag ccgccactgt taactttttc atatccgctc cgtcgttatg
    55141 gttataaaaa cgtgacgaat atacctacat tggagtaaca aatgatccta catagatcac
    55201 attacatatg ataattcacc atttattgat ctggattaat ttagaagttg cattgagaat
    55261 cctggttaat gtgatttaaa tcaattttta aatattttct ctacgcaaat attaactcga
    55321 tcgccacttg ccgcgctttg cccacgcctg aaggccagca attacctccg ctttacaaag
    55381 atatgctttt ccatacaaga cctgatgctg ccacccgagg cttaaccagg gtacaattgc
    55441 ccgctaatta acacctgcat aaaactcaag gagagtgcat gtctatcacg gcgcagtccg
    55501 tataccgtga caccggaaat ttcttccgta atcaatttat gaccattctg ttggtatcgt
    55561 tgctatgtgc gtttatcaca gtggtgttag ggcatgtttt ctcacccagt gatgcacagc
    55621 ttgcgcagct caatgacggc gtgcccgtta gcggcagtag tgggttgttc gacctggttc
    55681 agaatatgtc accggaacag caacaaattt tgctgcaggc ttcagcggcg tccacttttt
    55741 cagaattaat cggtaacgcc attctcgccg gaggcgtaat attaattatc cagctggtgt
    55801 ctgcgggtca gagagtcagt gcgctgcgag ctattggtgc cagtgcgccg atattgccca
    55861 agttatttat tctgattttt ctgactaccc ttttagtaca gattggcatc atgctggtgg
    55921 tcgttccggg aattatcatg gccattttac tggccctggc accggtgatg ctggtacagg
    55981 acaaaatggg catttttgcc tcgatgcgta gcagtatgcg gctgacttgg gcgaatatgc
    56041 gtctggtggc acccgcagta ctgagctggt tgctggcaaa aacactgttg ctgctttttg
    56101 cctcttcttt tgccgcatta accccggaaa ttggtgccgt actggcgaac accttgagca
    56161 acctgatttc tgccatattg ctcatctatc tgttccgcct gtatatgttg attcgccaat
    56221 aaccttaagc atttgattac ggaatcgtaa aatgaagcag tttcttgatt ttttaccgct
    56281 ggttgtcttt ttcgcgtttt acaagattta tgacatctat gcggctactg cagcgctgat
    56341 cgtcgccacg gcgattgtgc ttatctatag ctgggttcgc tttcgtaagg ttgagaagat
    56401 ggccctgatc acttttgttc tggtggtcgt cttcggtggc ttgacgctgt tcttccacaa
    56461 tgatgagttt attaaatgga aggttacagt catttatgct ctgtttgcgg gagccctgtt
    56521 agtcagccaa tgggtgatga aaaagccgct aattcagcgg atgctgagta aagaactcac
    56581 gctgccgcaa ccggtatggt cgaagctaaa tctggcctgg gctgttttct ttatcctttg
    56641 cggtctggca aacatctaca tcgcattctg gctgccgcaa aatatttggg tcaactttaa
    56701 agtctttggc ctgaccgccc ttaccttaat cttcacattg ttaagcggta tctatatcta
    56761 ccgccacatg ccgcaggaag ataaatccta accagactgc cagtccttcg gggctggcgc
    56821 tgtatctcct ctgttctcct caggaaaatc atagtagcat cgcgcctgtg attttccttt
    56881 taagtcggtt ttaccatgtc tacaacacat aacgtccctc agggcgatct tgttttacgt
    56941 actttagcca tgcccgccga taccaatgcc aatggtgaca tctttggtgg ttggttaatg
    57001 tcacaaatgg atattggcgg cgctattctg gcaaaagaaa ttgcccacgg tcgcgtagtg
    57061 actgtgcggg ttgaaggaat gactttctta cggccggttg cggtcggcga tgtggtgtgc
    57121 tgctatgcac gctgtgtcca gaaagggacg acatcggtca gcattaatat tgaagtgtgg
    57181 gtgaaaaaag tagcgtctga accaatcggg caacgctata aagcgacaga agcattattt
    57241 aagtatgtag cggttgatcc tgaaggaaaa cctcgcgcct tacctgttga gtaatagtca
    57301 aaagcctccg gtcggaggct tttgactttc tgcttactga atttcggtgg tgccgttaat
    57361 tttaaacagg atattcacca caatcccact gcctggctta cccggctcat aacgccatct
    57421 gcgcatcgca tttttcacct cacgctcaaa catgttcgca ggcttggctg agaggatttg
    57481 tacgttatcc acgcgaccat ccggcgtgac gtcaaattta actttaacct gcccttcaat
    57541 gcgcaatgcc tgtgctcgtg ccggatactg cggctgatta cggcttaatg cgcgtggtcc
    57601 tgaagccaca ctggtaaccg gcttgctggt tgcagccgtt gccgtacttg atgtggggcg
    57661 cgcgggtgcc gtattttcaa acggtgatgc cggacgcgac tctacgggtt tgacatcgcg
    57721 ttttggctgc tcctgtacct ttttcaccgg cttcggtttt ggcttaggtt tcggcttcgg
    57781 cttttcaatg accaccggtg cttcttttgg cggttcgggg atcggctcag gttccggttc
    57841 tggctctacc accggctccg gtggcggctg aacggcttgt ggcggttcga gatcagcagg
    57901 cgcaaccatc gtgacagaaa ttggttgcgc aggcgcaggt agttcaataa cctgatgtac
    57961 cgaggtatag agcagccccg ccacaacagc accatgaatg cagaccgaaa gtaacgtcgg
    58021 ccaggggaag cggcgaggta aatcaagggt tattgaagtc ataatcattt cagtagaaaa
    58081 actaggcctc gattttaaat gcaaatagca atcatattca ataaggcaag acgatcagga
    58141 tcagtgaaaa tgtcattttt ggcgtaatta cccttacttt atgcatgacc ttaacaaaga
    58201 catcatcttt ccgttgcaga tcttcgccct ttcaaataac gtactttaca actttcccga
    58261 acaaggagtt gtgcccgtgt tgtatgttat ttacgctcaa gacaaagctg atagcctcga
    58321 aaaacgcctt tccgttcgtc cggcacattt agcacgttta cagttactgc atgatgaagg
    58381 tcgtttgttg acagct
//
LOCUS       DAJTQQ010000014        57940 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000014 DAJTQQ010000000
VERSION     DAJTQQ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57940
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.014"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(26..280)
                     /locus_tag="OO309_000949"
     CDS             complement(26..280)
                     /locus_tag="OO309_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR5846188.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            435..1007
                     /gene="yeiP"
                     /locus_tag="OO309_000950"
     CDS             435..1007
                     /gene="yeiP"
                     /locus_tag="OO309_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR5846189.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1230..2696
                     /locus_tag="OO309_000951"
     CDS             1230..2696
                     /locus_tag="OO309_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5846190.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2814..3800
                     /gene="yeiR"
                     /locus_tag="OO309_000952"
     CDS             2814..3800
                     /gene="yeiR"
                     /locus_tag="OO309_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR5846191.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3839..4552
                     /gene="lpxT"
                     /locus_tag="OO309_000953"
     CDS             3839..4552
                     /gene="lpxT"
                     /locus_tag="OO309_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR5846192.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4964..5530
                     /gene="mepS"
                     /locus_tag="OO309_000954"
     CDS             4964..5530
                     /gene="mepS"
                     /locus_tag="OO309_000954"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR5846193.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5711..7267
                     /locus_tag="OO309_000955"
     CDS             5711..7267
                     /locus_tag="OO309_000955"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR5846194.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVHETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7349..9163
                     /locus_tag="OO309_000956"
     CDS             7349..9163
                     /locus_tag="OO309_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5846195.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9164..10258
                     /locus_tag="OO309_000957"
     CDS             9164..10258
                     /locus_tag="OO309_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR5846196.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10258..11283
                     /locus_tag="OO309_000958"
     CDS             10258..11283
                     /locus_tag="OO309_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5846197.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11285..12874
                     /gene="yejF"
                     /locus_tag="OO309_000959"
     CDS             11285..12874
                     /gene="yejF"
                     /locus_tag="OO309_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR5846198.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12878..13222)
                     /locus_tag="OO309_000960"
     CDS             complement(12878..13222)
                     /locus_tag="OO309_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846199.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13555..14745)
                     /gene="bcr"
                     /locus_tag="OO309_000961"
     CDS             complement(13555..14745)
                     /gene="bcr"
                     /locus_tag="OO309_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR5846200.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14773..15468)
                     /gene="rsuA"
                     /locus_tag="OO309_000962"
     CDS             complement(14773..15468)
                     /gene="rsuA"
                     /locus_tag="OO309_000962"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR5846201.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15618..17378
                     /locus_tag="OO309_000963"
     CDS             15618..17378
                     /locus_tag="OO309_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5846202.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17503..17787
                     /gene="rplY"
                     /locus_tag="OO309_000964"
     CDS             17503..17787
                     /gene="rplY"
                     /locus_tag="OO309_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR5846203.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17926..18933)
                     /gene="yejK"
                     /locus_tag="OO309_000965"
     CDS             complement(17926..18933)
                     /gene="yejK"
                     /locus_tag="OO309_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR5846204.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19115..19342
                     /locus_tag="OO309_000966"
     CDS             19115..19342
                     /locus_tag="OO309_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR5846205.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19362..21122
                     /gene="pbgA"
                     /locus_tag="OO309_000967"
     CDS             19362..21122
                     /gene="pbgA"
                     /locus_tag="OO309_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR5846206.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21197..21273
                     /locus_tag="OO309_000968"
     tRNA            21197..21273
                     /locus_tag="OO309_000968"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21231..21233,aa:Pro,seq:ggg)
     gene            complement(21376..23967)
                     /gene="yejO"
                     /locus_tag="OO309_000969"
     CDS             complement(21376..23967)
                     /gene="yejO"
                     /locus_tag="OO309_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR5846207.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24287..24934
                     /gene="narP"
                     /locus_tag="OO309_000970"
     CDS             24287..24934
                     /gene="narP"
                     /locus_tag="OO309_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR5846208.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25145..26197)
                     /gene="ccmH"
                     /locus_tag="OO309_000971"
     CDS             complement(25145..26197)
                     /gene="ccmH"
                     /locus_tag="OO309_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR5846209.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26194..26751)
                     /gene="dsbE"
                     /locus_tag="OO309_000972"
     CDS             complement(26194..26751)
                     /gene="dsbE"
                     /locus_tag="OO309_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR5846210.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26748..28691)
                     /gene="ccmF"
                     /locus_tag="OO309_000973"
     CDS             complement(26748..28691)
                     /gene="ccmF"
                     /locus_tag="OO309_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR5846211.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28688..29167)
                     /gene="ccmE"
                     /locus_tag="OO309_000974"
     CDS             complement(28688..29167)
                     /gene="ccmE"
                     /locus_tag="OO309_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR5846212.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29164..29373)
                     /gene="ccmD"
                     /locus_tag="OO309_000975"
     CDS             complement(29164..29373)
                     /gene="ccmD"
                     /locus_tag="OO309_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR5846213.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29370..30107)
                     /gene="ccmC"
                     /locus_tag="OO309_000976"
     CDS             complement(29370..30107)
                     /gene="ccmC"
                     /locus_tag="OO309_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR5846214.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     IMVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30149..30811)
                     /gene="ccmB"
                     /locus_tag="OO309_000977"
     CDS             complement(30149..30811)
                     /gene="ccmB"
                     /locus_tag="OO309_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR5846215.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30808..31425)
                     /gene="ccmA"
                     /locus_tag="OO309_000978"
     CDS             complement(30808..31425)
                     /gene="ccmA"
                     /locus_tag="OO309_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR5846216.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31444..32046)
                     /gene="napC"
                     /locus_tag="OO309_000979"
     CDS             complement(31444..32046)
                     /gene="napC"
                     /locus_tag="OO309_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR5846217.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32056..32505)
                     /gene="napB"
                     /locus_tag="OO309_000980"
     CDS             complement(32056..32505)
                     /gene="napB"
                     /locus_tag="OO309_000980"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR5846218.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32502..33365)
                     /gene="napH"
                     /locus_tag="OO309_000981"
     CDS             complement(32502..33365)
                     /gene="napH"
                     /locus_tag="OO309_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR5846219.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33352..34047)
                     /gene="napG"
                     /locus_tag="OO309_000982"
     CDS             complement(33352..34047)
                     /gene="napG"
                     /locus_tag="OO309_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR5846220.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34054..36540)
                     /gene="napA"
                     /locus_tag="OO309_000983"
     CDS             complement(34054..36540)
                     /gene="napA"
                     /locus_tag="OO309_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR5846221.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36537..36800)
                     /gene="napD"
                     /locus_tag="OO309_000984"
     CDS             complement(36537..36800)
                     /gene="napD"
                     /locus_tag="OO309_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR5846222.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36790..37284)
                     /gene="napF"
                     /locus_tag="OO309_000985"
     CDS             complement(36790..37284)
                     /gene="napF"
                     /locus_tag="OO309_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR5846223.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37393..37557
                     /locus_tag="OO309_000986"
     CDS             37393..37557
                     /locus_tag="OO309_000986"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846224.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37692..38180
                     /gene="eco"
                     /locus_tag="OO309_000987"
     CDS             37692..38180
                     /gene="eco"
                     /locus_tag="OO309_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR5846225.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38302..39333)
                     /locus_tag="OO309_000988"
     CDS             complement(38302..39333)
                     /locus_tag="OO309_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCR5846226.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39567..39926)
                     /locus_tag="OO309_000989"
                     /pseudo
     CDS             complement(39567..39926)
                     /locus_tag="OO309_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40144..41787)
                     /gene="yojI"
                     /locus_tag="OO309_000990"
     CDS             complement(40144..41787)
                     /gene="yojI"
                     /locus_tag="OO309_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR5846227.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41863..42513)
                     /gene="alkB"
                     /locus_tag="OO309_000991"
     CDS             complement(41863..42513)
                     /gene="alkB"
                     /locus_tag="OO309_000991"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR5846228.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42513..43577)
                     /gene="ada"
                     /locus_tag="OO309_000992"
     CDS             complement(42513..43577)
                     /gene="ada"
                     /locus_tag="OO309_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR5846229.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43651..44706)
                     /gene="apbE"
                     /locus_tag="OO309_000993"
     CDS             complement(43651..44706)
                     /gene="apbE"
                     /locus_tag="OO309_000993"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR5846230.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44818..45939)
                     /gene="ompC"
                     /locus_tag="OO309_000994"
     CDS             complement(44818..45939)
                     /gene="ompC"
                     /locus_tag="OO309_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5846231.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46677..49349
                     /gene="rcsD"
                     /locus_tag="OO309_000995"
     CDS             46677..49349
                     /gene="rcsD"
                     /locus_tag="OO309_000995"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR5846232.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49366..50016
                     /gene="rcsB"
                     /locus_tag="OO309_000996"
     CDS             49366..50016
                     /gene="rcsB"
                     /locus_tag="OO309_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR5846233.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50216..53065)
                     /gene="rcsC"
                     /locus_tag="OO309_000997"
     CDS             complement(50216..53065)
                     /gene="rcsC"
                     /locus_tag="OO309_000997"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR5846234.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53340..54116)
                     /locus_tag="OO309_000998"
     CDS             complement(53340..54116)
                     /locus_tag="OO309_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR5846235.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54121..55770)
                     /locus_tag="OO309_000999"
     CDS             complement(54121..55770)
                     /locus_tag="OO309_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR5846236.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55771..56330)
                     /locus_tag="OO309_001000"
                     /pseudo
     CDS             complement(55771..56330)
                     /locus_tag="OO309_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56489..>57940)
                     /locus_tag="OO309_001001"
     CDS             complement(56489..>57940)
                     /locus_tag="OO309_001001"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5846237.1"
                     /translation="PNIGKFFYPLGRNNVRTSSSLSFISYDQALSSEPVAPGATNRSE
                     RRVKMLERPRREEVDTAAWMPSLTTDKQGKAYFTFLMPDSLTRWRITARGMNGDGLVG
                     QGRAYLRSEKNLYMKWSMPTVYRVGDKPAAGLFIFSQQDNEPVALVTKFAGAEMRQTL
                     TLHKGANYISLTQNIQQSGLLSAELQQNGQVQDSISTKLSFVDNSWPVEQQKNVMLGG
                     GENALMLPEQASNIRLQSSETPQEIFRNNLDALVDEPWGGVINTGSRLIPLSLAWRSL
                     ADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGEDGNGDAFLTAWAWYADWQASQ
                     ALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQEMNLPCKTLLKGGDEAIARR
                     GTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTMTLLKKAQLKSTVMPQVQQYA
                     WDKAANSNQPLAHTVVLLNSGGDATQTAAILMV"
BASE COUNT        14176 a        15630 c        14235 g        13899 t
ORIGIN      
        1 tttgcttact gcggattaaa gatgtttaag gcgcggtcag catttccaga tcgataagcc
       61 aggtcatggc ctgctgacgc gagttaccgc acatttcagc gctggcttgt aagcctgcac
      121 agactttcgg tcgcagtggc gaggtgaaaa ttttacagcg ttgctgttca tcaagctgaa
      181 tgcagggcgt attggcgggc ttgccatctg gcataccggg aatagggctg gaaattgaag
      241 gggcagtgca acacgccccg caacccggac ggcattccat acgttgttct cttaaggtta
      301 accaccggat tgatgagcgc gcacagtacc accttttttg caccagcaaa agtgcgaata
      361 ccacttgccc gaaaggcccg tcgcgagtac tttgtcgcga tatttttgac attttcgact
      421 acaggaattt ttcgatgcca agagcgaacg aaattaaaaa aggtatggta ctgaattaca
      481 acggcaaact gctgctggct aaggatattg atattcagtc gcccactgcc cgcggtgccg
      541 ctacgctgta caaaatgcgt ttttctgatg tccgtaccgg gctgaaagta gaagagcgtt
      601 tcaaaggtga tgatatcgtt gacaccgtga cgctgacccg ccgttacgtt gacttctcct
      661 atgtcgatgg caacgaatat gtctttatgg ataaagaaga ctataccccg tataccttca
      721 ccaaagatca gattgaagaa gagttgctgt ttatgccaga gggcggcatg ccggacatgc
      781 aggtgctgac ctgggacggt caactgctgg cgcttgagct gccgcagacc gttgatctgg
      841 aaatcgttga aaccgcacca ggcatcaaag gggcatctgc cagcgcccgt aacaaaccgg
      901 cgacattgag cactggtctg gtgattcagg taccagaata cttaagcccg ggcgaaaaaa
      961 ttcgtatcca tatcgaagaa cgccgttata tggggcgtgc tgactaactt cagccgcatg
     1021 cagaaaaggg atagctcagg ctgtcccttt tttaatttat tataccaatc ttctattttg
     1081 cgcttcctga acaccttacg ccctcaattt tcactcgttg attgatcgcc ctcacacttc
     1141 atcgcattaa caatccagac caacttcaat tgctgtcata taactttaca ctgtcattgt
     1201 taatttatcg ttactaagac gtgactccta tgaagacaat tgcctccgtt acgctcccgc
     1261 atcatgtaca cgctccacgc tacgatcgcc agcagttgca atcacgtatc gttcattttg
     1321 gctttggcgc ctttcaccgc gctcatcagg cgttactgac cgatcgtgtg ctgaatgccc
     1381 agggcggcga ctgggggatc tgtgaaatca gcttgttcag cggtgatcaa ctgatgagcc
     1441 agctccgcgc acagaaccat ttatataccg tgctggagaa aggtgcggac ggcaatcagg
     1501 tgataattgt cggtgccgtt cacgaatgcc ttaatgcaaa actggattcc ttagcggcaa
     1561 ttattgagaa attttgcgag ccacaggtgg caattgtttc cctgacgatt accgaaaaag
     1621 gctattgtat tgacccggcc accggtgcac tcgacaccag taatccgcgg attattcacg
     1681 atctacaaac ccctgaagaa cctcactccg caccgggtat tctcgtcgaa gcactgaaac
     1741 gccgccgtga gcgcagcctt acaccgttta ccgtgctctc ctgcgacaat attcccgaca
     1801 atggccatgt ggtgaaaaac gcggtgctga gaatggcaga aaaacgttcg ccagaactcg
     1861 ccgggtggat aaaagaacac gtcagttttc cgggaaccat ggtcgaccgc attgttccgg
     1921 ctgcaaccga cgaatcactg gcagaaatca gccagcatct gggggtaaat gatccctgcg
     1981 cgattagctg cgaaccgttt atccagtggg tggttgaaga taacttcatc gctgggcgtc
     2041 ctgcctggga agtcgcaggt gtacaaatgg tgaatgatgt cctgccatgg gaagagatga
     2101 aactgcgtat gcttaatggc agccactctt ttctcgctta tctgggttac ctctcaggat
     2161 tcgcctatat cagtgattgc atgcaggatc gcgtatttcg ctatgctgcc agaacattaa
     2221 tgctggatga gcaagcgccg acactgcaaa ttaaagatgt cgatttaaca caatatgcgg
     2281 ataagttaat tgcacgtttt gctaatccgt cgctgaaaca taagacctgg caaatcgcga
     2341 tggatggcag ccagaaatta ccgcaacgta tgctggcagg tattcgcata catctggggc
     2401 gtgaaacgga ctggtcgttg ctggcattag gcgttgctgg ctggatgcgt tacgtcagcg
     2461 gcgttgatga tgccggaaat gccattgatg ttcgcgatcc gcttagcgat aaaattcgcg
     2521 aacttgttgc gggcagcagc agtgaacaac gcgtaaccgc cctgctttcc ctgcgtgaag
     2581 ttttcggtga tgatctgcca gataacccgc attttgtgca ggccatcgaa caagcctggc
     2641 aacaaatcgt acaattcggc gcacatcagg cgctattaaa caccctcaaa atttaacgat
     2701 ttctgcggtt aaagcggatg aagctcacct tcgtccgctc tccccttctc ttttctgcct
     2761 tttttagcca ggattaacgc tcagttaact taccagagta ttgcggagcc gttgtgacca
     2821 ggaccaacct catcaccggt tttctcggca gcgggaaaac cacgtcgatt cttcatctgt
     2881 tagcccataa agatcccaac gaaaaatggg cggtactggt taatgaattt ggggaagtcg
     2941 gaattgatgg tgctttgctc gccgatagcg gcgcattgct gaaagagatc cccggcggct
     3001 gcatgtgctg cgttaatggt ttacccatgc aggtagggtt gaatacctta ctgcgtcagg
     3061 gaaaaccaga ccgcttgttg atagagccga ccgggctggg ccatccgaaa cagatcctcg
     3121 atctgttaac cgcaccagtc tatgaaccgt ggatagatct gcgcgccacc ttgtgcattc
     3181 tcgatccacg cctgctgctg gacgaaaaaa gcgccagcaa tgaaaacttc cgtgaccagc
     3241 tggctgccgc agacatcatt gtcgccaata aatccgaccg tgcgacgccc gaaagtgagc
     3301 aagcgctaca gcgttggtgg cagcaaaatg gtggcgatcg acaattaatt cacagtgagc
     3361 atgggaaagt tgacggtcat cttctggatt tgccgcgtcg caatttagcc gagttgcccg
     3421 ccagcgccgc gcattctcat cagcatgtcg tgaaaaaagg gttagcagcg ttaagcctgc
     3481 cagagcatca acgctggcgt cgcagtctga acagcgggca aggatatcag gcctgcggct
     3541 ggatattcga cgctgatacg gtattcgaca ccattggcat tctggaatgg gcgcgacttg
     3601 caccggtgga acgcgtcaaa ggcgtgctgc gtattcccga agggctggta cgaatcaacc
     3661 gtcagggcga tgacctgcac attgaaacgc aaaacgttgc gccaccggac agccgtattg
     3721 agctgatttc cagcagcgaa gctgactgga atgccttgca gagcgcgctg ttgaagcttc
     3781 gtttagcgac taccgcgtaa ggttgcctgc gtttttcagt aagataatta gagaaaatat
     3841 gattaaaaat ttgccgcaaa tagtgttgtt gaatattgtc ggccccgcgc tgtttctttc
     3901 ctggtatatt cccgttaatc atggattctg gttgccgatt gatgcggata ttttttattt
     3961 ctttaatcag aaactggtcg aaagtaaggc ctttttatgg ctggttgcat tgactaacaa
     4021 tcgcgccttc gacggttgtt cactgctggc gatgggtatg ttgatgctga gtttctggct
     4081 gaaagaaaac gcccctggca gacgacgtat cgtgattatg ggtctggtca tgctattaac
     4141 tgcagtggta ttaaaccagc tgggtcaggc attaattcct gtaaaacggg ccagcccaac
     4201 attgactttt accgatatta accgcgtcag cgaactgctc tctgttccca cgaaagatgc
     4261 ctcacgagat agctttcccg gcgatcacgg catgatgctg cttatttttt cggcattcat
     4321 gtggcgttat ttcggcaaag ttgcaggcct tatcgccctt attattttta tggtttttgc
     4381 atttcccaga gtaatgattg gcgcacactg gtttactgac atcattgtcg gttcgatgac
     4441 cgtgatattg atcggtttgc cctgggtgtt gctgacgcca ttaagtgatc gattaatcac
     4501 cttttttgac aaatcactac caggaaaaaa caaacatttc caaaacaaat aactcacagt
     4561 aattaacatc atcagggtta tttttatagt gaggataatc ctgatgatgc gcaccgtgct
     4621 ttcatctatc gaacgcaaaa atcattctct aagtaaatga atggattgca tgcgtttcac
     4681 tcaattgtac tttaattgac caaccccgct tattaacttt ctgtatcact ttttcttata
     4741 aaaaatcatg taaaaccgct cgccaagacc gcaccaatcg ggtaatctcg aactcgtttt
     4801 gcctcggcgg tagattatcc tcacagcata taattttgtg cgttagtcca cagatttggc
     4861 cttaaggaat tgtttcaaca tgcccaggta attagtctcg tgtcgcttgg cattttttta
     4921 taacgatatt tgtcgttaag gacttcaagg gaaaacaaac aacatggtca aatctcaacc
     4981 gattttgaga tatatcttgc gcgggattcc cgcgattgca gtagcggttc tgctttctgc
     5041 atgtagtgca aataacaccg caaagaatat gcatcctgag acacgtgcag tgggtagtga
     5101 aacatcatca ctgcaagctt ctcaggatga atttgaaaac ctggttcgta atgtcgacgt
     5161 aaaatcgcga attatggatc agtatgctga ctggaaaggc gtacgttatc gtctgggcgg
     5221 cagcactaaa aaaggtatcg attgttctgg tttcgtacag cgtacattcc gtgagcaatt
     5281 tggcttagaa cttccgcgtt cgacttacga acagcaggaa atgggtaaat ctgtttcccg
     5341 cagtaatttg cgtacgggtg atttagttct gttccgtgcc ggttcaacgg gacgccatgt
     5401 cggtatttat atcggcaaca atcagtttgt ccatgcttcc accagcagtg gtgttattat
     5461 ttccagcatg aatgaaccgt actggaagaa gcgttacaac gaagcacgcc gggttctcag
     5521 ccgcagctaa taaaccgttt ggatgcaatc ccttggctat cctgacgagt taactgaaag
     5581 cactgcttag gcagtgcttt tttgttttca ttcatcagag aaaatgatgt ttccgcgtct
     5641 tgatccaggc tatagtccgg tcattgttat cttttaaatg ttgtcgtaat ttcaggaaat
     5701 taacggaatc atgttcatac gcgctcccaa ttctggacgt aagctcctgc ttacctgcat
     5761 tgtagcaggc gtgatgattg cgatactggt tagctgcctt cagtttttag tggcctggca
     5821 taagcacgaa gtcaaatacg acacactgat tatcgacgta caaaagtatc tcgataccta
     5881 ttttgccgac ctgaaatcca ctactgaccg gcttcagccg ctgaccttag atacctgcca
     5941 gcaggctaac cccgaactga ccgctcgcgc ggcgtttagc atgaatgtcc gtacgtttgt
     6001 gctggtgaaa gataaaaaaa cattctgttc atctgcgact ggtgagatgg acattccact
     6061 caatgaattg attccggcgc tcgacattaa taaaaacgtc gatatggcga tcttacccgg
     6121 cacgccgatg gtgccgaaca aacccgcaat cgtcatctgg tatcgcaacc ctttgctgaa
     6181 aaatagcggt gtgtttgccg ctctgaatct caacctgacg ccttctctct tttatagttc
     6241 acggcaggaa gattacgatg gcgtcgccct cattattggc aatactgcgc tatctacctt
     6301 ttcttcacgt ttgatgaacg ttaatgaatt aaccgacatg ccggtccatg aaactaaaat
     6361 tgcgggcatt cctctgaccg ttcggcttta tgcggatgac tggacatgga acgatgtgtg
     6421 gtacgcattt ttactgggcg gcatgagtgg aactgtcgtt ggcctgcttt gctattacct
     6481 gatgagcgta cgtatgcgcc ccgacagaga aatcatgacc gccatcaagc gcgaacaatt
     6541 ttacgtggcg tatcaaccgg tggtggatac acaagctttg cgggtaacgg gcctggaagt
     6601 actgctacgc tggcggcatc ctgtagcggg agaaattccc ccggatgcct tcattaactt
     6661 tgccgaatcg caaaagatga ttgtgccgct gactcagcac ctgtttgagt taattgcccg
     6721 cgatgccgca gaattagaaa aagtactgcc ggtaggcgtc aaatttggca ttaacattgc
     6781 gccggaccat ctgcacagcg aaagctttaa agcagatatc cagaaactgc tcacttccct
     6841 gcccgcacac catttccaga ttgtgctgga aattaccgag cgcgatatgc tgaaagagca
     6901 agaagccaca caactcttcg cctggctgca ctcggtcggc gtagaaattg ctattgatga
     6961 cttcggcacc gggcacagcg cgcttatcta tcttgagcgt tttacgctcg attatctgaa
     7021 aattgaccgt ggatttatca acgccatcgg tacggaaacg atcacttcac ccgtacttga
     7081 cgcggtgctg acgctggcga aacgtctcaa tatgctgaca gttactgaag gggtcgaaac
     7141 gccggaacaa gcgcgatggc taagcgaacg cggcgttaat ttcatgcaag gctactggat
     7201 tagtcacccg ttaccgctgg acgattttgt tcgctggctg aagaaaccgt atacgccgca
     7261 gtggtaaggt gtgcttacgt cccttattat tcatagtgaa agcatgccgg attgcggcta
     7321 atgatgagta aaaggaaatc cgttgcagat gattgtgcgc atactgctgc tgtttatcgc
     7381 tctgttcacc ttcggtgcgc aggcgcaggc tatcaaggaa agctatacct ttgccgtgct
     7441 gggcgaaccc cggtacgcgt ttaatttcaa ccattttgat tatgtgaacc ccgccgcgcc
     7501 aaaaggtggg cagataacgt tgtcagccct cggtaccttc gataatttca accgctatgc
     7561 actgcgcggc aacccgggcg cacgcaccga gcagctgtac gacacgttat ttacgacttc
     7621 cgatgacgaa ccaggcagtt attacccgct gattgctgaa agtgcacgct atgctgacga
     7681 ttattcctgg gtggaggtcg ctattaatcc gcgcgcccgt tttcatgatg gttctcccat
     7741 tactgcccgc gatgtagagt ttacttttca aaaatttctg accgaaggcg tgccgcaatt
     7801 tcgtctggtc tacaaaggca ccaccgtcaa agccattgcg ccgttaaccg tgcgcattga
     7861 gttagctaaa cccggcaaag aagatatgtt gagtctgttt tcgctgccgg tatttccaga
     7921 aaagtactgg aaggatcaca aacttagcga cccgctcgcc acgcctccgc ttgccagtgg
     7981 tccgtaccac gttacgtcct ggaaaatggg gcaaaatatt gtctattccc gcgtgaaaga
     8041 ttactgggca gcaaacttac cggtaaaccg tggacgctgg aatttcgaca ccattcgcta
     8101 cgattattac ctcgatgata atgtcgcctt tgaagcgttt aaagcaggtg cctttgattt
     8161 gcgtatggaa aacgacgcta aaaactgggc cacgcgttat accggtaaaa atttcgataa
     8221 aaaatacatc atcaaagatg agcaaaagaa cgaatcagcc caggatacgc gctggctggc
     8281 gtttaatatc caacgtgcgg tattcagcga tcgccgggtg cgggaagcaa tcactctcgc
     8341 ctttgacttt gaatggatga acaaggcgtt gttttacaac gcctggagtc gcacgaacag
     8401 ttattttcaa aataccgaat acgcggccag aaattacccc gacgccgcgg agctggtgct
     8461 tctggcacca atgaaaaaag atctaccgcc agaagtcttc acacaaatct accagccgcc
     8521 ggtatctaaa ggcgatggct acgatcgtga caacctgtta aaagccgaca aacttctcaa
     8581 cgaagcgggc tgggtgctga agggtcagca acgcgttaat gccacaacgg gtcagccgct
     8641 cagctttgaa ttattgcttc ccgcaagcag caatagtcag tgggtattgc cgttccagca
     8701 cagcctgcaa cggctgggta tcaatatgga cattcgcaag gtggataact cgcaaatcac
     8761 taaccgcatg cgcaatcgcg actatgacat gatgccgcga ctatggcggg cgatgccgtg
     8821 gcccagttcc gatttacaga tttcctggtc atcggaatat atcaattcca cttataatgc
     8881 ccccggcgtg caaagcccgg ttatcgactc actgatcaac caaattattg ccgcgcaggg
     8941 aaataaagaa aaattactgc cgttggggcg agcactggat cgcgtattaa cgtggaatta
     9001 ttacatgctg ccaatgtggt acatggcgga agaccgtctc gcctggtggg ataaattctc
     9061 ccaacccgct gtacgccctg tttacagcct gggtatcgat acctggtggt atgacgttaa
     9121 taaagcggcc aaactgccgt cagccagaca acagggagag tagatgggcg cttacctgat
     9181 tcgccgtctg ttgctggtga tcccaacgtt atgggctatt atcactatca actttttcat
     9241 cgtgcaaatt gcgcctggcg gtccggtcga tcaggccatc gccgccattg agtttggtaa
     9301 tgccggagta ttacccggcg caggcggtga aggtgttcgt gccagccatg cgcaaacggg
     9361 tgtcggcaat atcagcgaca gtaattaccg tggcggacgc ggattagatc cagaagtgat
     9421 cgctgagatc actcatcgct acggtttcga taagccgatc cacgaacgtt acttcaaaat
     9481 gctctgggac tacatccgct ttgattttgg tgatagcctg tttcgcagcg cctcggtgct
     9541 gacgctgatt aaagacagtc tgccggtttc catcaccctc ggattgtgga gcacgctgat
     9601 tatctatctg gtgtcgattc cgttaggcat tcgcaaagct gtttataatg ggagtcgctt
     9661 tgacgtctgg agtagcgcat ttatcatcat cggctacgcc attccggcct ttttgtttgc
     9721 catcctgctg attgtcttct tcgcgggcgg cagctatttc gacctgttcc ctctacgcgg
     9781 cctggtttcc gctaactttg attcgctgcc gtggtatcag aaaatcaccg attatctgtg
     9841 gcatatcacg ctaccggtgc tggcgacagt gattggtggc tttgcggcgc tgaccatgct
     9901 gaccaaaaac tcattccttg atgaagtgcg caagcaatac gtggtgaccg cgcgtgcgaa
     9961 aggggtaagt gagaaaaaca ttctctggaa acatatgttc cgcaacgcca tgctgctggt
    10021 gattgccggt tttccggcga cgtttatcag catgtttttt accggctcgc tgctgattga
    10081 ggtgatgttt tcactcaatg gtcttggctt actgggctac gaagcgaccg tctcgcgcga
    10141 ttatcctgta atgtttggta ccttgtatat tttcaccctg attggcctgc tgctgaatat
    10201 tgtcagtgat atcagctata cgctggttga tccgcgtata gattttgagg gacgttaatg
    10261 tcgcgactca gccccgtcaa tcaggcccgt tgggcgcgtt ttcgtcataa ccgtcgcggc
    10321 tactggtcgt tatggatttt cctcgttttg tttggtttga gtttgtgttc tgaacttatc
    10381 gctaacgata aaccgttgct ggtgcgttat gacggcagtt ggtatttccc gttgttgaaa
    10441 aactacagcg aaagcgattt tggcggcccg ctggcaagtc aggctgatta tcaggacccg
    10501 tggctgaaac aacggctgga aaataacggc tgggtactgt gggcaccgat tcgctttggt
    10561 gctaccagta tcaactttgc taccgataag cccttccctt ctccaccctc ccggcaaaac
    10621 tggctgggaa cggatgcaaa cggcggcgat gtgctggcac gcattctcta tggcacgcgg
    10681 atctcggttc tgtttggcct gatgctgact ctctgttcca gcgtgatggg cgtgctggcg
    10741 ggggcgctac aaggctatta cggcggtaaa gtcgatctct gggggcaacg ctttattgaa
    10801 gtatggtcgg ggatgccgac gctgtttttg attattttac tttccagcgt cgtacagcct
    10861 aacttctggt ggctgctggc aattactgtc ttgtttggct ggatgagtct ggtcggcgtg
    10921 gtgcgggcgg agtttttacg tactcgtaat ttcgactaca ttcgtgcggc acaggcgctt
    10981 ggcgtcagcg atcgcagtat catcctgcgt catatgttgc ctaatgccat ggtcgcgacc
    11041 ctcacctttt taccgtttat tttatgtagt tcgataacca ccctgacctc gctcgatttc
    11101 ctcggcttcg gtctaccgct cggttcaccg tcactcggtg aactgctgtt acaagggaaa
    11161 aataaccttc aggccccgtg gcttgggatc accgccttct tgtcggtggc gatattattg
    11221 tctttgctga tctttattgg tgaagccgtc cgcgacgcat ttgatcctaa taaggcggtg
    11281 tagcatgacg caaactctgt tagcgattga aaatttgtcg gtgggttttc gccatcagca
    11341 aaccgtacgt acagtagtca atgatgtttc actacagatt gaggctggcg aaacgctggc
    11401 gctggtgggt gagtcaggtt caggcaaaag cgttaccgcg ctgtcaattt tacgcctgct
    11461 cccttccccg ccggttgaat atctctccgg cgatattcgt tttcatggcg aatcgctgct
    11521 tcatgccagc gatcaaacgt tgcgcggtgt acgcggtaat aagatcgcca tgatttttca
    11581 ggaaccgatg gtgtcgttaa atccattgca taccctggaa aaacagcttt atgaagtgct
    11641 ttcactccac cgcgggatgc gtcgggaagc ggctcgtggc gaaattctta actgccttga
    11701 tcgcgtcggt atccgccagg cggcaaaacg gctgacagat tatccgcatc agctctccgg
    11761 cggcgaacgc cagcgggtga tgattgcgat ggcgctgtta acgcgaccgg aattattaat
    11821 tgccgatgaa ccgaccaccg cactggacgt ctctgtccag gcgcagattt tacagctgtt
    11881 gcgcgaactg caaggcgagc tgaatatggg catgctgttt attactcata acctcagcat
    11941 tgtcagaaaa ctggcccacc gcgtggcggt aatgcaaaac ggtcgctgtg tcgagcaaaa
    12001 taacgccgct acgctatttg cctcacccac tcatccttac acacaaaagc tactcaacag
    12061 tgaaccgtca ggcgatccgg taccgttgcc agaacctgcc tcaacgttgc tggatgttga
    12121 acagcttcag gttgccttcc ccattcgcaa agggattttg aagcgcattg tggatcataa
    12181 tgtggtggtg aaaaacatca gttttacgct acgggcgggt gaaacactgg gtttagtggg
    12241 cgagtctggt tccgggaaaa gcacgacggg actggcgctg ctgcgactga ttacctctca
    12301 gggcagcatc gtctttgacg gtcagccact gcaagattta aatcgccgcc agctgttacc
    12361 tactcgtcat cgtattcagg tggtatttca ggatccaaac tcctcgctca acccacgact
    12421 caacgttttg cagattattg aggaaggctt acgggttcac cagccgacgc tttctgccgc
    12481 acaacgcgaa caacaagtga tagccgtgat gcatgaagtg ggattagatc ctgaaacacg
    12541 ccaccgttat ccggcggagt tctctggtgg tcagcgacaa cgtattgcga ttgccagggc
    12601 attaattctt aagccctcgc tgatcatact tgatgaaccg acatcatcac tcgacaaaac
    12661 ggtacaggcg caaatattga cgcttttgaa atcattgcaa caaaagcatc aactggccta
    12721 tttgtttatc agccacgatt tgcacgttgt ccgcgcgtta tgtcatcagg ttatcgtact
    12781 gcgacaaggg gaagtagtgg aacaaggacc gtgcgcgcgc gtgtttgcca caccgcagca
    12841 ggagtatacg cgtcagttac tggcgttgag ctgacgctta aaaaggattg tagtttgaaa
    12901 agggttcggc aattgccaca ccaaaatttt tcaatcggca tgttgcagca aactcgtcct
    12961 ggcgatttac aaacaggcac ggctcacctt cacactccaa caccgaacat ggaacttcga
    13021 tgtcgcttaa cgcctggctt agtttataca tcaccgacca tgcattatca ccatccgggc
    13081 taagcaattt aagcgccacc aggctgttgt caccgcacgg tccattttga ggaatcggtt
    13141 caaccttcga acctgcgaaa ccagcagacc agcgatagtt ctgcggcagt cgatggacga
    13201 ttgagagttg taatgaagtc acttattttc cccggaagta cattcacttc acaatttgtt
    13261 tacatcaatt ttaacacatc atcaacaatc cgtctttata cagatcgtaa agattgatgc
    13321 ctcgcctgcg tggcctctat ggctctgatt taagtaatat ctgcgggcgt ggtcacgttt
    13381 gcggggctat atgtacccgt ttctgcgatc taactcaacc tttttaacta caatgatgtg
    13441 actttttaca taaattgatt ttacataaaa taaacatata tcggggaaat gatagatttg
    13501 tggttgacat gcatcaacaa aggaagcctt ttaccttcct cgttgtgcaa tagatcaccg
    13561 ttttttcggc cgactggcgt acagacagaa gagaatggag ctggttgcgc agaatgcaat
    13621 tgaccaaatc atcggccatg cagagttaaa ggtcgcaaga gaaagcaatg cgccaacaat
    13681 tgcccctatg ccaaaacgga aggttcctgc cagcgaagat gccgttcccg ccatatgggg
    13741 aaactcatca agaatgaccg ccatcgcatt ggatgacacc atcgacacgc agcccacaaa
    13801 cgccgcaacg ccaaccacca gcgaccagaa tcccagcccc agcggcgcac tgatgaccat
    13861 ccacgcagcc ataataaatt gtatccacaa ccccgagcgg aacatattta acgcgccaat
    13921 gcggcggacg aagcggctgt taaagatggt catcacgaac agaaaaacaa tgtttagcgc
    13981 aaagtaataa ccaaagtttt ccggcgcgac gtggttaatt tcaatataaa caaacggtcc
    14041 ggcgcttaag aatgagaaca tcccggcaaa gctgaaacca ctggcaagca tgtagctcag
    14101 gacacgttta tggcggaaca gcgccgcaaa gttaccaata gtggtacgaa tgtgaaatgg
    14161 ctgacgacgc tccggtggta aggtttcttt aatcaggaag aaaatcattg ccgaagccag
    14221 aatcgccgct aatgccagga tccagaagat gtaatgccag ctcagccaca ccagcaccca
    14281 gccgccaact atcggtgcca tcagcagtgc aatggtcgtc accagcatga caaacgacat
    14341 catccgcgag aactcttctt tcgggtaaat atcacgcatc aaagcgttga taaccacgct
    14401 ggccgcagcc gcagccagcc cgtggaagaa acgcatcaca atcagctgat cgatggtttg
    14461 tgccaacgca cacgccaccg cggcggcggc aaacaccagc gtcccgccaa gaaccaccgg
    14521 cttacgcccg aagctgtctg ccatcggccc gtagattaac tgccccaacg caaagcccag
    14581 aatataagta ctgagggtca tctgcgtact gcccgccgat acgccaaact gcgctgaaat
    14641 taccggtagc gcgggcagat acatatcaat cgacagcggc atcaacatgg ccagcaggcc
    14701 aaggataaaa acaatagcaa acgacgaatg ctgtcgggtg gtcacaacgg gctcctgaaa
    14761 gtcattgaaa agttagacga cgctggcaat ttcttcttca gttaacggac gatattcacc
    14821 gggggctaaa tcagcatcca gcgtaatacc gccaatacgt tcacgatgca gctcaaccac
    14881 gtggttaccc acggcggcga acatgcgttt cacctgatga taacgccctt cgctgatggt
    14941 cagacgaacc tgcgttgggg taatcacttc aagcaccgcc ggcttagtga gatctttttc
    15001 gttatgcagc tgcacgcctt tagcaaattg ctctgccgta tcgtcagcta caggtgattc
    15061 cagtgtcacc agataggtct tctcgcaatg atggcgcgga gaagtaatgc ggtgcgacca
    15121 ctgaccatca tcagtcatca gcaccagacc ggtggtatcg atatccagac gtcctgcggc
    15181 gtgcagtttc cacgctaccg gttcatccag aaaatagagc accgtcgggt gatccgggtc
    15241 gtccgtggag caaacatagc cctgaggctt attgagcatg aagtaacgtg gaccgtgttg
    15301 ctgcgccagc gggttgccat cgtaagcgac atcatgttca gggagcagtt tgaacgctgc
    15361 attacgaacg atttcgccat cgacggtgac acgattgccg cggatttcac gcccggcaat
    15421 agcacggcta acgccgagtt gctgtgcgat aaatttatca agtcgcatgt gtgtgatttt
    15481 gcctgtaaaa aacggaggtc gggtaatgac ccgaaaatct gaactgttgt ctgcccagta
    15541 tagcggtcta ataacttccc tcaagggaaa aagaaccgtg gcatactatt agcagaataa
    15601 tctacctacg cgaaaccatg atttttacac tccgcccata tcagcaagaa gccgtggatg
    15661 ccacgctcaa ccattttcgt cgtcataaaa cccctgccgt tatcgtgctg cccaccggcg
    15721 caggtaaaag cctggtgata gcggaactgg cacggctggc gcgtggtcgc gtactggtgc
    15781 tggcacacgt taaagaactg gtggcgcaaa accatgcaaa gtatcaggcg ctggggctgg
    15841 aagccgatat ttttgccgcc gggctaaagc gcaaagagag ccacggtaaa gtggtatttg
    15901 gcagcgttca gtcggtcgcc cgtaatcttg atgcctttca gggtgaattt tcgctgttga
    15961 ttgtcgatga atgccaccgt attggtgacg atgaagagag ccagtatcag caaatcctca
    16021 ctcacctgac taaagtgaat ccccacttac gcctgctggg gctgactgcc acgccttttc
    16081 gattgggcaa aggctggatc taccagttcc attatcacgg catggtacgc ggcgatgaga
    16141 aagccctttt ccgtgactgc atttatgagc tgccgctgcg ttatatgatt aaacacggct
    16201 atctgacgcc gccagaacga ctggatatgc cagtagtgca atacgatttc agccgcttgc
    16261 aggcacagag taacgggctg ttcagcgaag ctgatctcaa ccgtgagctg aaaaaacagc
    16321 aacgcattac gccacatatc atcagccaga tcatggagtt cgccgaaaag cgcaaagggg
    16381 taatgatttt cgccgccacc gttgaacacg caaaagagat tgtgggatta ctgcctgccg
    16441 aagatgctgc actgattact ggcgacaacc ccggcgctga gcgcgatgtg ttaattgaag
    16501 attttaaagc ccagcgtttt cgctatctgg tcaacgtcgc agtactgacc accggatttg
    16561 acgccccgca cgtcgatctt atcgccattc tgcgccctac cgaatcagtg agtctttacc
    16621 aacaaattgt cgggcgcggt ctgcgtctcg ctccgggcaa gactgattgt ttaattcttg
    16681 attatgcggg taatcctcac gatctctacg cgccggaagt tggtacaccg aaaggcaaaa
    16741 gtgacaacgt tccggtacag gttttctgcc ctgcctgcgg ttttgccaac accttttggg
    16801 ggaaaacgac cgccgacggg acattgattg aacactttgg tcgccgctgt cagggatggt
    16861 ttgaagatga cgacggtcat cgcgaacagt gtgacttccg tttccgtttt aaaaattgcc
    16921 cgcaatgtaa cgcagaaaat gatattgccg ctcgccgctg ccgcgaatgt gacacggtac
    16981 tggttgatcc ggatgatatg ttaaaagcgg cgctacgact gaaagatgcg ctggtattac
    17041 gctgtagcgg catgtctttg cagcatgggc acgacgagaa aggcgaatgg ttgaaaatca
    17101 cctattacga tgaagacggc gcggatgtga gtgagcgttt ccgtctgcaa acgcccgccc
    17161 agcgtaccgc cttcgagcag ctttttatcc gcccgcatac gcgcacaccg ggcatccctc
    17221 tgcgctggat caccgccgcc gatatcctcg cccagcaagc cttactgcga catccggatt
    17281 ttgtcgtcgc ccgcatgaaa ggtcagtact ggcaagtgcg tgaaaaagtg ttcgattacg
    17341 aaggtcgttt tcgtcgggcg cacgaattac gcggttaatg gtagttttga ttgatgtata
    17401 aggcgattga gtatagaatc tcgcccgctt ttgcatacgc aaagcagatc acttacctgt
    17461 tgctgggtcg cctgtagcag gattaattta agagagaaag aaatgtttac tatcaacgca
    17521 gaagtacgta aagagcaggg taagggtgcg agccgccgcc tgcgtgccgc taacaagttc
    17581 ccggcaatca tctacggtgg caaagaagca ccgctggcta tcgagctgga tcacgacaaa
    17641 gtcatgaaca tgcaagctaa agctgaattc tacagcgaag ttctaaccat cgttgttgac
    17701 ggtaaagaaa tcaaagttaa agctcaggac gtacagcgtc acccgtacaa accgaagctg
    17761 cagcacatcg acttcgttcg cgcttaattg ctgaataagt tgtaaaaaac cccgctccgg
    17821 cggggttttt tgtatctgca gattatgcct gattacggta ttgctatttt ttgcaggcca
    17881 gataaggcgt tcacgccgca tctggcattc agaacacaaa accgattaat tgccgccaga
    17941 cgtccggcgc tgcaattggt cgcgcaaatt cggcggcgtg cctttaatgg tcaaggtatc
    18001 ggttgctgga tcccagaaga tgcgttcacc taacagcatc gcatcaaagt taatagtcaa
    18061 gccaccgccg ctaccggcaa acttcgtcaa ctgacgcagt gtgctacggt ctgccgggaa
    18121 gctctcttcc agttcgtagc ctttttcagc cgcaaactcc gtgaagctca cttcgcttac
    18181 gcctgccagc tctttcgaca gcgattccag ttcgatctct tcgcctgcct gcagctgctc
    18241 attacagtag ctataaacct gctggcgcac gttctgtcgc tctgctttat ccagttgtgc
    18301 ttcggcagtg aaatcatcaa ccgcttgcaa cagcccacgg ttttgcgctt tcgcgttcaa
    18361 tccttcgctg gccccgagga aatccataaa gaaatccgcc actttgcgcc ctactcgccc
    18421 tttcaggaaa gtgagatagc gggtggactc tggattggtt tcccattcgg ttaaatcgat
    18481 acgcgcaaca atatccgcat gattgatatc aagataatgg gttgggttga tgtcgagatt
    18541 ttcgttaacg cgcatactgc tcaggttgct cagaactgcc accagcaaat actccaccgc
    18601 cagataacgg tagtggcaaa acagcacgaa tccaccgtca gcaaaaggat atttcgctaa
    18661 ttcgtcacgc aggcgaccgg ttgccgcgcg gctaaatgcc agaaagtcct cttcgccctg
    18721 acgctgtaag cgcagcgtct gcgccagttc actctcttcg ctaaacaagc cgtaagcttt
    18781 atttttggcg ctatagaccc gatgcagttc tgccaccatc tcaacgacgg tttctgtcgg
    18841 ttccagcaat gaatcgcgca acaccagctc aaggttctgc tcatcacgct tgataagctg
    18901 gtgcagagca atctggttga tatccagact catgataaac tctcctttaa gaccgggcgg
    18961 tattcaacca ccgccagtta agcgacgcaa taaaagatgg cggtcatttt acagaaggca
    19021 gcgtaacgag aatgatttaa cgcataaaaa agcagaaaaa aaaccgttgc tacggtaata
    19081 tgttgccctt tcatcaacta actgatttcg atttatgcca caaatttccc gctacagtaa
    19141 tgaacaggtt gaacaattgc tcgcagagct gctcaacgta ctggaaaaac ataaggctcc
    19201 gaccgacctt tccctgatgg tgttaggcaa catggtcact aaccttatca acaccagcat
    19261 tgccccggcc caacgccagg caattgccaa ctcttttgcc agcgccttac agtcctctat
    19321 caacgaagac aaagcgcact aagggaaaca gataacaggt tatggtaact catcgtcagc
    19381 gctaccgtga aaaagtctcc cagatggtca gttgggggca ctggtttgca ctgttcaata
    19441 ttctgctttc gctcgtcatt ggcagccgtt acctgtttat cgccgactgg ccgacaacgc
    19501 ttgctggtcg catttattcc tacgtaagca ttatcggcca tttcagcttc ctagtgttcg
    19561 ccacctactt gctgatcctc ttcccgctga cctttatcgt cggctcccag aggctgatga
    19621 ggtttttgtc cgtcattctg gcgacggcgg gaatgacgct attactgatc gatagcgaag
    19681 tctttactcg tttccatctc catcttaatc ccatcgtctg gcagttggtt atcaacccag
    19741 acgaaaatga gatggcgcgc gactggcagc tgatgttcat cagcgtgccg gttattttat
    19801 tgcttgaact ggtgtttgcg acgtggagct ggcaaaagct gcgcagcctg acgcgtcgtc
    19861 gacgcttcgc gcgcccgctg gccgcattct tatttatcgc ctttatcgcc tcgcatgtgg
    19921 tgtatatctg ggccgatgcc aacttctatc gcccgatcac catgcagcgc gctaacctgc
    19981 cgctttcgta cccgatgacg gcgcgacgtt tccttgagaa gcatggcctg cttgatgcgc
    20041 aggagtatca acgccgtctt attgagcaag gtaatccaga cgccgtttcc gtacagtatc
    20101 cgttaagcga actgcgctat cgcgatatgg gcaccgggca gaatgtgctg ttgattactg
    20161 tcgatggcct gaactactca cgcttcgaga agcagatgcc tgcgctggca ggttttgctg
    20221 agcaaaatat ttcgttcacg cgccatatga gctccggcaa cactacagac aacggcatct
    20281 ttggcctgtt ctatggcatc tcgccgagct atatggacgg cattctgtcg acccgtacgc
    20341 ctgcggcatt aattactgcg cttaatcagc aaggctatca gctggggtta ttctcatcag
    20401 atggctttac cagcccgctg tatcgccagg cattgttgtc agatttctcg atgccgagcg
    20461 tacgcaccca atctgacgat cagaccgcca cgcagtggat caactggctg ggccgctacg
    20521 cacaagaaga taaccgctgg ttctcgtggg tttctttcaa tggcactaac attgacgaca
    20581 gcaatcagca ggcatttgca cggaaatata gccgggcggc aggcaatgtc gatgaccaga
    20641 tcaaacgcgt gctcaatgca ctgcgtgatt ctggcaaact ggacaatacg gtggtgatta
    20701 tcactgccgg tcggggtatt ccactgagcg aagaggaaga aacctttgac tggtcccacg
    20761 gtcatctgca ggtgccatta gtgattcact ggccaggcac gccggcgcag cgtattaatg
    20821 ctctgactga tcataccgat ctgatgacga cgctgatgca acgcctgctg catgtcagca
    20881 cacctgccag cgaatattcg caaggtcagg atttgttcaa ccctcaacgc cgtcattact
    20941 gggttactgc tgcggataac gatacgctgg caattaccac cccgaaaaag acgctggtgc
    21001 tgaacaataa cggtaaatac cgcacttaca acttacgtgg tgaaagagtg aaagatgaaa
    21061 aaccacagtt aagtttgtta ttgcaagtgc tgacagacga gaagcgtttt atcgctaact
    21121 gattaattat aaatcagtta gcgaaatatc ttacttgcaa tcggtgtgga aaacggtagt
    21181 attagcagcc acgagtcggc acgtagcgca gcctggtagc gcaccgtcat ggggtgtcgg
    21241 gggtcggagg ttcaaatcct ctcgtgccga ccaaaaatcc caagaaaaaa ccaaccctta
    21301 cggttggttt ttttatatct gcaattaatt cgataaacag accgtgacac atcacagcct
    21361 gtttattttc tgttatcaga acgtccagac cacacccgcc tgagtattcc acggcgattc
    21421 tacccctgcg ccattgccat agctgactga caaatgaccg cttaacgtcg gggtaaacga
    21481 tgaccttatc ccagcctgat aaacgccact ggttccagcc acatcattat taaatttgcc
    21541 atcgtcatta actttcacct ggttagaatc ggcgtattcc tgacgcacgg cggctttcag
    21601 ccagggttcc agcgtcgtac cgttttgcag gtccatgtga tagcttaccg ccgttcccgc
    21661 ttcagcgcgt aatatccggg tatttcccac atccgcgcgc atgccgtttg ataacgtgta
    21721 gtcctgacca tctgtggtaa agccggtaaa ggccagatag ggtctaacac tccacaatcc
    21781 gtcaacccaa cggaacccgc tctcaacatg agcacccgcg ccgttactat tgtaatcgcc
    21841 aaacgctgtt gccccattac tcatcttgcc atggatggtg ttggcaaaac ggtcaacttt
    21901 caccacccca tcaacatagg caccgttctg atgctcccaa ccggcataag cccccagggt
    21961 atagctatcg atattacctt ttccgccgcg atcaaaacca atatcagaat gagagtaacc
    22021 aaagaacaag ccgcgaattg tactgctttc ttcacgggag aaacggctat cgataccgag
    22081 cgtcaggccc gtcaatgttt gctcaaaacc agctcccgca tcagtggtca cgttgttgcg
    22141 ggtgttaatt gccgaactcc acatcaccgt atcgtaacta acgcctttta cgctaccaag
    22201 acgctcacgc acggtgtcca gttctgcatc aaataccagc ggttgtgcgg ctgccatgtt
    22261 cagcacatca gtggttgaag gggtaatttg cgcgcgattc tctgcgaggc tccagctatg
    22321 gttgccatta tccagcaagg tatattcata cgtaccgata tcaacaacgc ctccggcatt
    22381 gcccaacgta aatgcagcat caccgccgcc cgttgttacc agtgtaaggc tatctcctgc
    22441 tgccgggctg gcaccggtgt ccgtcacgaa tattttgaaa tcacctgttg cctgaccggt
    22501 gacgttgagc tgatcgctct gatgattggc catatcggta cgcatataaa aattgccgtt
    22561 tcccgacagg gtattggtgg tcaacgtaat gaaattttcc gcacttgccg tcacagatga
    22621 acgagtcact ggctcagcca tcataatcac atttgcgccg gacatcgcaa ggttggctaa
    22681 gttgaggcga gtgttttggt tcgtcgtagt gatatcggca atgatggtcc atgcgctatt
    22741 ttcgagcgaa acgtccgctt tgctggtatc cacggcacca tgcgttctaa aagaagcgcc
    22801 ctcagaaacc actaccttac ccaccaacat agcgggtgca taatttattt gcggcttcat
    22861 gacctcaaga atgccatcat tccctctgac tgacacgtta accattgtgc cagcccagac
    22921 attagcgcgg ccattgttga ttaccatatt ttcagagatg gcttttgagg aatacgtgat
    22981 acttccattc tggtctgatc gaccgagatc atataccgca tttgcctgca ctttcgtacc
    23041 agtatccttt cccagcgatt gcatagtggc atgctcatca aggatagtgt caatggcctg
    23101 cgtgtcctcc ataacaatga gcccggaacc atcatccagt tcataatttt ttgacacacc
    23161 atcctttata ctaaattggc ctttactgtt tgtaccactc acttccagcg cattcgttga
    23221 gacaattaat ttcccgcctg cttttttatc aacgccagtt gcagtcccgc catccataac
    23281 ctccagtaag ccgccactat ccacagtagt attataagcg aagtcatttt cttcaactcg
    23341 caaacttccg ccgttttcca acaacatatt attagcaata ccattcttga tatcgaactg
    23401 accgagacgg tttgttccga atacctccat ggcccgcgtg gttgttttaa tagcaccgcc
    23461 tgttttctga tctaccgcaa gcgcaaatcc cccctcttta acctccagta gtcctccctt
    23521 ttcgatagcg gcaaaaacgg cccccccatc tttttcaacg attaagcgcc cgcctttaat
    23581 cgtggtatca ttcgctgggc cataaacctg tagcacacca ttattgaggg tagtctggtt
    23641 tgccgcgcca cccatttgaa caatttgata accatcattt aatactgagt attctgcggt
    23701 accattcatt tcaatgtatt gataccctcc gttaatttca gtattactac ttacaccaaa
    23761 ttcttttata tgctgttctc cgccaggatt gattttcgta ttattggcgg tcccataaac
    23821 cagctggata tctttttcca ggataacacc atcaactgtc tcaccatact caactgttga
    23881 tgccaatgct atgggtgaat ataacgctgt agccaccaga acagatattg cggaacgaca
    23941 aagagaaaca gaaccagatt gatgcattga gctttcatcc tatgaaatta attgcagtta
    24001 aaagcattgg gtacagaaaa tacccatagc tccatacccg gagtcagttt ttaaaaactg
    24061 tttaaagaaa tgcacaagta ttgtgattga ttttttagtt gtttttcttg atgagaagct
    24121 gatgcaaaat tccgtcttta taataaaaat gatgccaaag cgaacgacaa ggttgtagtt
    24181 ttcactacat gtccatacat aaaatggggt aacattcacg cgcctggtag cgttaccaac
    24241 gctacgctca aacataatga ttctaataaa acctcaggag actactatgc ctgaagcaac
    24301 gccttttcag gtgatgattg tggatgatca tccacttatg cgacgcggtg ttcgtcagtt
    24361 actggagctt gatcctggct ttgaagtggt cgccgaagcg ggcgaaggcg cgagcgcgat
    24421 cgatctggcg aatagactgg atatcgacgt gatcttgctg gatctcaata tgaaaggtat
    24481 gagtggcctg gataccctca atgccttgcg cagggatggc gttaccgcgc aaattattat
    24541 cctgaccgta tccgatgcct ccagcgatgt ctttgcgctg atagacgcag gcgcagacgg
    24601 ttatctgttg aaagacagcg acccggaagt attgctggaa gcgattcgta ccggagcgaa
    24661 aggcagcaaa gtctttagcg aacgcgtcaa tcagtactta cgtgaacgtg aaatgtttgg
    24721 cgcggaagaa gatcccttca gcgtgctgac ggagcgcgag ctggatgttc tgcacgagct
    24781 ggcacagggg ctgtcaaata aacagattgc atcggtgttg aatatttccg agcagacagt
    24841 aaaagtacat attcgcaatc tgctgcgtaa actcaatgtc cgctcacgcg tggcggccac
    24901 cattctgttc ctgcaacaac gcggggcaca ataaaaatag cccgatggat ttatcatcgg
    24961 gctgagattt atgacaaacg caaaactgcc tgatgcgcta cgcttatcag gcctacgtgg
    25021 atcgatcaat ttattgaatt tacacaattt tgtaggccgg ataaggcgtt cacgccgcat
    25081 ccggcataaa ctaagcgcac tttgtcatca gtctagcccg atggcatcac catcgggcct
    25141 ctttttattt actctcctgc ggcgacaaat gttgcatcgc ctgcgcgata ctacgttcaa
    25201 tcaccgcacg gcgagtatcg ttggcaggta agagtttcaa catcatctcc cacgcggcaa
    25261 cggcttcgcc aaatcgctgc tgctcaaagg cattaaacgc atacatgctt agcacacgga
    25321 tattgctatg gtccgttctc accagctgac gtagcagttc accgccgagg cggttgtcgt
    25381 tgggatcaga tgaacgagtc aacgcttcag cgtatcccag tgcggcatcg ctgttcttcg
    25441 gatcgaggcg atacgcagta gcgtatgcat cggtggcgat actggcgtta cccagcgcca
    25501 tgccaacgcg gcccaacata atccagcctt ctatatctcc cggatttttt tgcagttgag
    25561 tacgcatccc cagcgcaagg cgcgacatct cttcttcgtt gagcggatcg gctttcgggt
    25621 ccagcgccct gtccagcaac gccggagcct gtgccgtggc ctgctgccag attttcacct
    25681 gctgataatt gccagtctgg tagtagctga cgccagccac aattaacgcc accacaatac
    25741 ccggcagata aacaacatat ccggcacgct taccttccgg cacgctttgt tcaggaaacg
    25801 cttccggcac cacgcgtacc cgacgccgcg agcgggcgta tatgacccaa ccgccaatgc
    25861 caatagccac tactggcagc acccacagca gcacggtcag cggcgttaac ggcggatcgt
    25921 aagtgacgaa gttgccgtaa cgcgccacca tataatcgac aatctctttt ttacttttac
    25981 cttcctgcat cagttcatac actttctgac gcaggtcggt ggcaatcatc gagttggaat
    26041 cggcaatgct gttgttctgg catttcgggc agcgcagttc ttcagtgagc tgacggaact
    26101 gttgttcctg tgcttcatct ttaaactgca acacgtcgat ggtcgccagc gctgagccgg
    26161 agatcatcag catcagcacg cccaataaaa acctcattgt gcggcctcct tactgtattt
    26221 ctcccacagc ggcttgatct cttcttccca gacgcgagga ttgagatcgc ccgcatggcg
    26281 atagcgaatg ataccgttgc cgtcaataag gaaggtttct ggcgcgccat agacaccgag
    26341 atccagccct aacatgccgt cgccatcaaa caggcttagc gcgtaaggat tgcccagctc
    26401 tttcagccag ctgattgcct tctggcgatc gtctttatag ttcatgccga ccacgcgaat
    26461 gccctgcgct gaaagctgat tcagatattg atgttccgca cggcaggtcg gacaccaagt
    26521 cgcccagacg ttaagcagta ctggtttgcc ctgagtcagc acatccgcct gataaaactg
    26581 ccccggattg tccagtgatt cgagacgaaa cttcggcaca ggcttgccaa tgagcgccga
    26641 ttccagattg gtcggatcat ccccttcggc attacgcgcc agctgccaca gcagcgccgc
    26701 ggcaatcgcc aggaagataa tcaacggaat taacaatact ttgcgcttca tacggcctcc
    26761 ggcgcagttt tttgcggact cacgcgctta cgatagcgag gatcaaacag acacagcagt
    26821 ccgcccaacg ccatcatcag cccgcccgcc caaatccagc gaacaaatgg tttgtagtaa
    26881 agacgcacgg cccacgcgcc gttttccagc tcttcaccga gcgcggcgta caggtcacgc
    26941 gtgatgccgc cgtcaattgc cgcttcggtc atcatcgacc cggcagtgtt gtaataacgt
    27001 ttttccgcat acagcaccgt ttccggttta ccctctcgcg ttacgccaat agtcgccaca
    27061 ccgccgcgcc agttcgggcc agtcacctct ttgacatcac ggaaggtgaa gcgatattca
    27121 tgaatatcga cgctatcgcc ggacttcatg cgcacatcac gctcaacgct ataattctgg
    27181 ctaaaggcaa tgccaacaat tgtcactgcc agccccaggt gagccgccac catcccccaa
    27241 taactgaagg tggttttcgt gccgcgtgaa atacgcagcg cagcttccgc agttgccagc
    27301 accgcaatcc agcaggccat tgccaggccg agcaccgtca tcgccacaac tttgctttcg
    27361 aacagccacg gcaacagcag agacagcacc agcgtagaga tgaaggcgat aatcaataaa
    27421 ttgcggattt tacgtgggcg atcgcgcccc cagcgcacca gaggaccgac accaagcagt
    27481 agcgcaaacg gcaccatcag ccaggtaaac atggtgttga agaacggttc gccaatcgaa
    27541 atactgccca gtccaagttg cttgtgcacc agcggcagca gcgtccccag caacaccacc
    27601 agcatcgcgg cgaccagcaa aacgttgttc gctaacagca aagattcccg cgaccacagc
    27661 gcattgttta cgcgtgagcg aactttgtgt ccacgcgcgg caaacagcag cagcgaaccg
    27721 ccaatcacca gcaccataaa ggcgaggata aacataccgc gcgccggatc agacgcaaac
    27781 gcgtgtaccg ataccagcac gccggaacgc acgaggaaag tccccaacag acacaacgag
    27841 aaggcactga ttgccagcag taatgtccac gctttgaagc tggcgcgttg ttcagtgacc
    27901 gccagtgagt gcatcagtgc agtccccacc agccacggca taaacgaggc gttttctacc
    27961 ggatcccaga accaccagcc accccagccg agttcgtaat aggcccatgc ggaaccgagc
    28021 acaatgccga gcgttaggaa aatccacgcc gccagcgtcc acgggcgagt aaaacgcgca
    28081 taagtgctgt ccagacgccc gctcagcaaa gaagcaatgg caaaagcaaa cgccaccgag
    28141 aaacccacgt accccatata aagcagaggc ggatggaaga tcagccccgg atcctgtaac
    28201 agcgggttaa gatcgcgccc ttcaatcggg aagttcggca gcgtgcgtga gaacgggtta
    28261 gaggtaaaga gaatgaacag caaaaagccg acgctgacca tccccattat cgccagtaca
    28321 cgggccacaa tatccagcgg aatacgctga ctaaaaatcg ccaccgcaaa ggtccagccg
    28381 ctcatcagca gcacccacag cagaagcgaa ccttcatgcg cgccccaagt agccgccacg
    28441 cgataccaca ccggaagctg ggtattggag ttgctggcaa cataggtgac ggtaaagtcg
    28501 ttgaccacga aagcattgac cagcaccaga aatgcgccag ccacagacat aaacagcagc
    28561 caggcaaaca agcgggaaga cgccatcatg cgcgcatctc cgcgcgccac gccccatagc
    28621 ggatacacgg acagcagcag cgcaatccct aacgccaagc acagcagccc gtttccaatt
    28681 tccggcatca tgatgctggg tccttataaa cactcgccgg gcgacggtgg ttagcttcca
    28741 tcgctttctc aacttctggc ggcgtgtagt tttcgtcgtg tttcgccagc acttctttcg
    28801 cgaggatatg attgcctttt tccagctcgc cctgcaccac aacgccctgc ccttcacgga
    28861 acagatccgg caaaatgcct tcgtaagaga catccactga gccttcagca tcgtaaatgg
    28921 tgaaggtcac tttcagcgaa ttgggatcgc gctgcacgct acccggcatc accatcccgc
    28981 caacgcgcag acgctgaccg acttccggca tctgctgagt ttcacgcttg ccgtagagaa
    29041 tttcccccgg cgtataaaag agatcgatat tcgagcgcag cgcatatagc accagaccga
    29101 tagtcagcgc tagccctgcc aacacggcac aggcaatcca caagcggttt ttacggcgaa
    29161 tattcatgca gcctcctgct gttgcgcagc acgtaaacgc gcctcacgcg cccgctgttg
    29221 cgccacacca cgcagaattg cgcgatgttg catcaccgag tgcacgacca aaaccaccag
    29281 cggaataacg gtcatcacca ccgccagcca gacaaaaaag gcgtaaccgc ccattgcgaa
    29341 aaattcattc caggaagcaa atgcaggggt catttacggc ctcttttcag tatcagttca
    29401 ctcacccacg gacggcgttt ttccatcagc aaaatcaaat tacgcatccg catcagcgtc
    29461 agcgtggcag acaggagcag gaagccaaaa atcgaccagc gcagcggcga acgcatcgcc
    29521 ggatcgatac tttgctgcat ccgcgttgat ccctgatgca gggtgttcca ccactccacg
    29581 gagtaatgaa taatcggcag attcaccacg ccaatcagca ccatgatacc tgccgcacgg
    29641 cccgccagac ggcggtcgtc gaaggcgtgc cacagggcaa tcacacccac atacaaaaac
    29701 agcagcacca gttcagaagt cagacgtgca tcccataccc accaggtgcc ccacatcggt
    29761 tttccccatg cagagccggt aaccagggca ataaaggtaa acacggcacc aatgggggcc
    29821 atcgccgcca ccgccaggtt ggccattttc atctgccaga caaggccaat aaacgccgcc
    29881 accgccattg atgcataaat gcccatcgac cagatcgccg caggcacatg caggtagata
    29941 atgcggtagc tatttccctg ctgataatca gccggagcaa agccgaatcc ccagatccag
    30001 ccgacggtaa gcacgaccac actggcaatt gccagccacg gtataaacca gccacagatt
    30061 tgatacagcc gtggtgggat cgccagttga tgcagtgttt tccacatagt ttcgatacca
    30121 gactcgaaca aaaatcagta atccagcgtt attgaatgct gattcgtaac gctgccgccg
    30181 tcgcaaaagg acttaatgtc gcggtgcctg ccagcaacgc gcctaaaatt gccagatacc
    30241 catcaacggg caaatgcata gaagccgcgt ccatcgcggc ggtggcaaag atgagtaatg
    30301 ggatagtcag cggtaacacc agtatgctga gcagcacacc accgcgctta agtcccactg
    30361 tcagcgccac gcccggtgca ccgagaaagc caagcgtagg cgttcccagc agcagcgtca
    30421 gcgccatcac ttgccagcca taaacatcca ttcccagtag cattgctacc agtggcgaaa
    30481 ggatgagtaa cggcagaccg gttaccatcc agtgcgccat caccttcgcc agcacaacgg
    30541 cgggcaaggg taacggcaac aacatcaatt gttcaagact gccgtcctgc aaatcgtcac
    30601 ggaacagtcg ttccagcgcc agcaaggatg aaagcagcgc agcaacccag ataatgcccg
    30661 gtgcaatacg cgccagcagt tgcggctccg gaccgatact gagcggaaaa agggtaatta
    30721 caatcaggaa gaaccacagc gggttggcga tttcggcgct atggcgaaac gctacgcgca
    30781 gttcaagacg gaaaatacgc cagaacatca tgcggccctc gtttgcgtca gtgaaatgcg
    30841 gcgaatttta ctttcagcaa cgttgagcgg ctggtgggta gtcagaatca caatcccccc
    30901 ctgctccgta tgctgcgcca tacgctgggt cagacgatcg acgccgttaa cgtcaatcgc
    30961 ggtaaaaggc tcgtcgagga tccataatgt ggcacgggtc aaccacagac gcgctaaagc
    31021 gacgcggcgt tgttgcccgg cagaaagttg atttaccgga atatcttcga atccggcaag
    31081 cccggcctgc gccaaggctt ccagacattg tgcggcatcg ccatcgcgat gataaaagtg
    31141 cagattttct aacgccgtca gccgggtttt gatccccggc tgatggccta tccataacag
    31201 ggtttgatgg tagctgtcgc gtacctgatg caagggctgc ccttgccaga gaacctcgcc
    31261 agcgtcaggg cgagacaacc ccgtcagcaa acggagaagc gttgtcttcc ccgcgccgtt
    31321 gctaccggtg atttgtaccc actctcctgc gttcagcgta aatgacaagc cactaaataa
    31381 ggttcgttca tcccgctcac aaagtaactc tctggcttca agcataccca cgcaataacc
    31441 ctgttaaaaa cctggctcga cttcacgcat atcgggcagc ttgtgcgcga tccctttatg
    31501 gcaatcaata caggtttgcc catctttcac cgcctggtca tgcatcttcg cggcaaccga
    31561 tttctgggcg gttgtatcca tatactcgaa gttgtgacag ttacggcact cctgcgagtt
    31621 attgtccttc atgcgccgcc actcattctg tgccatcgtc agacgatgag cttcaaattt
    31681 ctgcggcgtg tcaataacgc caaaaatttt accatacagc tctttacttg ctttgagctt
    31741 gcgtatcatc ttcggcacaa actcgtgcgg aacgtgacaa tccggacagg tcgcacggac
    31801 gccgctacgg ttgttgtagt gcacggaatc catgtattcc tgatacaccg tgttgcgcat
    31861 ttcgtggcag ctaatgcaga actcttcggt attggctttt tccatcccgg tgttaaagcc
    31921 cccccagaag acgatgccgc caacaaaacc gatcaacagc agcgtcccca gcgccagatg
    31981 gctgggggta cgccaccatt tccacaggcg cttaatcaga ccaggcttac ggtcagaatt
    32041 tcccataata acctcttatt tcccgtaacc ttttgatggg gtaaaggtat tccccacgat
    32101 tggcgcggta tcggcctgcg gtacgtgaca ttgcagacag aaataacgac gcggagccac
    32161 ttccgcgccc actttgccgt cgctgtccat aaagtgagta ggactgatac gcggcgcgcc
    32221 agtggtgcga tagctttcga caccgtggca ctgcaagcag cgattggtat tggtcgttac
    32281 ctgataacct tcaacgctat gcgggatcat tggcggctga ttcacatagt tcagcggcat
    32341 ccgatcctgc tcttttggca tccgaatggc cccttcctgc gtcccggata cttccggcga
    32401 ctggctaaaa tcgacgccat tagccgccca aaccgcgccg cttaccacca gggccagcat
    32461 cgccgtccat tgacacagcg ctttcttcag gtcatggctt ttcatgattt cgctcccgaa
    32521 ctccatcgtg tagttattgt aaatacatcc tcagaacaga catccacgca gcgaccgcac
    32581 gtcatgcaat cgcggctggt gacctgcacc gggctttgtt catccagcac cggggcacgt
    32641 agcacatgcg gttccgggca aacatgaaaa caatccatac agcggttaca tttctgccga
    32701 tcgctggccg caacggtaat cacacctttg ctacccagca cgccatacag cgcgccaacc
    32761 ggacaaatgt gcccgcacca gccgtgttca acgaccagta gatcaaataa aaacagcgcg
    32821 agaataagca gcgcgccgct gccgaagccc attaccaggc tacgccccat cagagaaacg
    32881 gggttgatcc attcccaaat gagcgtgcca gttaaggctg atcccaccag aatgaccacc
    32941 aacagcacgt agcgaatgtg gcgagggatc gtcgcagact gattgaggtc aaagcgcctg
    33001 cgtaaccagt tcgctaagtc ggtaatcggg ttcagcgggc agacccagct gcaaaataat
    33061 cgctttcccg ccagggcgta gagcacggtg ataatcaccg caccggtcaa cgccaccgtg
    33121 gcaggcagat gaccactggc aagactttgc agcgtcatca gcggatcggt cagcggaacg
    33181 gtgtcgaata acaggctact gctgtagttg ccgtgcaaga tccacacacc aaaccatgga
    33241 ccgctcaaaa acatccccag cacgaagaac tgacaaaggc gacgtaacac cagccaacgg
    33301 tgactgcgcc accagccttt tttctccagc gcctcgcgcc cggcgtcacg tttacgattt
    33361 gccattgttc ccctccagcc agccgaagcg gtaatggtga cctaactccc ctttcgccag
    33421 tgacagcggt aacaccttga ttgccggttg ttccagcacg catacttttt cgcacttacc
    33481 gcaaccggta caggcgtcgc tgtgaaccgt cggcagaaag cgggcgtgct taccggtacg
    33541 cgtgttgcgc tccagctcca aggtgatggc ctcatcaatt ttcgggcatt cgcgataaca
    33601 aacatcgcag cgcagcccct gaaagttgag acagttttcc tggtccacca gtaccgccag
    33661 ccccatccgc gcgtcgtcga tcgattcaat ctcacgatcc agcgcaccgc ttgggcacac
    33721 tttggcgcac ggaatgtcct cacacatttc gcaaggaata tcccgtgcga caaaatatgg
    33781 cgtgcccgcc gacagaccag aggccagcgt cgccagtttt aaggtgtcgt aagggcaagc
    33841 ctgaacacac tgaccgcaac gcacacaggc actggcaaag gcgttctcgt ttatggctcc
    33901 gggcgggcgc aaccgcacgc cagatgcgcg tgcggtttgc tgttgtaacc ccagcgccac
    33961 acccacggca gccagcccgc ctgctgtgcg aacgacatcg cgcagaaagc ggcggcgacc
    34021 attttgaggt ttcgctgacc gggacataat ggcttacacc ttctccagtt tgaccgcgca
    34081 cttcttgaag tccgtctctt tcgagagcgg atcggtcgca tccagcgtca gtttgttaac
    34141 cagctgtgcg gcgtcgaaga acggcatgta caccaggccc tgtggcggac ggttacgacc
    34201 gcgcgtttca acaatcgaga tcacttcgcc acggcgagaa accactttca ctttgtcgcc
    34261 acggcgcaga tcgcgcgctt tcgcatccag cgggtgaata aacaagaccg cttccgggaa
    34321 ggcgcggtgc agttccggca cacggcgagt catactgccg gtgtgccagt gctccagaac
    34381 gcgtccggta gagagccaca ggtcgtactc ttcatccggt gcttccgccg ccggttcgaa
    34441 cggcagtgcg aagatcaccg ctttgccatc cggtttaccg tagaacttat agccttcgcc
    34501 cgctttcacg tacgggtcgt taccttcgct gtaacgccac tgtgtttctt taccattcac
    34561 caccggccag cgcagaccgc gcgctttgtg gtagtcatcg aacggtgcca gatcgtgacc
    34621 gtgaccgcga ccaaaccatg cgtactcttc gaacagccct ttttgcagat agaagcccag
    34681 ctcgcgggat tcatcgttca gctgatcttc cgccagttcg gataccggga atttgctcac
    34741 ttcgggggtg gcatacagaa cttcgtacag cgttttgcca cgcagttccg gtttcttcgc
    34801 cagcagctct tccggccata cttcttcagt tttgaagcgg cgggagaact ggactaactg
    34861 ccagagatcc gatttcgctt cgcctggcgc ctgtacctgc tgacgccaga actgagtacg
    34921 gcgttcggcg ttaccgtaag cgccctcttt ctctacccac attgcggtcg gcaggatcaa
    34981 gtcggcggcc agcgcactga ctgtcggata cggatcggag acgatgatga agttgcgcgg
    35041 atcgcgccag cccggcatac gctcttcatt gatgttcggc ccggcctgca tgttgttggt
    35101 acacatggtc cagtaaacat tcagcttgcc gtctttcagc gcacggtctt gtgccaccgc
    35161 atgaagaccg attttcgccg gaatggtgcc gctcgggata ttccacttct tctcacagat
    35221 atcacgatgt ttctcgttag ttaccaccat gtccgcaggc agacggtgag caaaggtgcc
    35281 cacttcacgc gcagtaccac acgcagaagg ttgcccggtc agggagaacg gaccgcaacc
    35341 cggctgggaa attttgccgg tcagcaggtg caggttgtag accaggttgt tagcccacac
    35401 accacgggta tgctggttga agcccatcgt ccagtaggag atgactttct tgttcggatc
    35461 ggcatacagc tgcgccagtt gttctaactg gtctttcggc acgccggtca tttcagcggt
    35521 tttttccagc gtatactcag caacgaaggc tttgtaatct tcaaagctca tcggttcgga
    35581 ggcgtcagaa cccggattct tcgctgcttt ttccagcgga tgggtcggac gtaaaccgta
    35641 gccgatgtcc gtcgcccctt tgcgcaggtt aacgtgcttg ctgaagaagt cctgatttat
    35701 cgcattgttt tgaatgatat agttggcgat gtagttcagg atcaccaggt cagattgcgg
    35761 cgtaaagatg atgccgttat ccgccagctc gaagctacga tgctggtagg tagaaagcac
    35821 cgccacggtg acgttctggt tagagagacg actgttagtg atgcgtgacc agaggatcgg
    35881 gtgcatctcc gccatgttag cgccccacag cacaaacgcg tcagcctgct cgatgtcgtc
    35941 atagcagccc atcggctcat ccataccaaa ggtacgcata aagccaacta ctgccgacgc
    36001 catacagtga cgtgcgttcg ggtcgatgtt gttcgaacgg aagcccgctt tgaacagctt
    36061 ggacgcggca taaccttccc agatagtcca ctgaccagaa ccgaacatac cgatcgattc
    36121 cggccctttt tctttcaggg cggttttgaa tttctcttcc atcacatcga aggcctgatc
    36181 ccaggtgatt ggggtaaatt cgccttcttt gtcatattta ccgtttttca tacgcagcag
    36241 cggctgcgtc aaacggtctt taccgtacat gatcttgggc aggaaatagc ccttaatgca
    36301 gttcaggcca cggttaaccg gtgcgtccgg gtcgccctga caggccacca cacgcccctg
    36361 ctgcgttccg accagaacgc cgcaaccagt accgcagaaa cggcacggcg ctttatccca
    36421 tttaatggct tcctgctgac caacaacggc gcgggcaacg cccggcacgc tgagaccggc
    36481 agccgccgca gcggccgcaa cggcgttagc tttcataaag ctacgacgac tgagtttcat
    36541 ggtgtttcct caccttgctc ttcctgctgg tgataaacca gcgacaccgc cagcacgccc
    36601 tctacgttgc gtactgactc aatggtttgg atcagcgttt cgctgtcttc tgcttccacc
    36661 accacaatca actgaccgct cggcgcgtcg ctgacagcaa cttcacagcc gggaaaggcg
    36721 ttcagttggg tgctgatgtc tgaaattcgt tcgcttttgg cctgcacgac caggctgcaa
    36781 acttgccagt tagtgtgcat ggagatactc cgcagttatg gctgataccg gacagctggc
    36841 tgcacatgct ccacatccgt tgcaaagttg gctattaagt tgcgcctggt agatcccgga
    36901 gagcgtcggg cgaaagataa ttgccattgg ctcacagcta tcctgacagc ggcggcattc
    36961 aactgactga tacgccaggc acgcgtcccc gatggtgaac tgcaaatccc aggccctggt
    37021 gtggcgcgga gaaaatagcg attcggggca agcctgcgcg caggcgtaac agaagctgca
    37081 ctcgttattt ttgaaattaa cgctcggata gccacccgtg ccgcgttgca gaatgttgtt
    37141 ttcacaagca ttaatacagg cgtcgcagcg ggtgcaatgg gtcagaaaat gagattcatc
    37201 accggaccag ggcggacgga taccgttact ggctttgcgc cagcaaccag tgagtatgcc
    37261 ccgacgggat gcatcaatct tcacattgac cttccatcat taacgcgctc tgaaaattga
    37321 gagcgaccaa atagaccgca taattaataa gccattttta tagccgctaa gatattaaag
    37381 gatgtgtcaa agatgcatac cccgatcggg gtaaaacctg tagcaggatc aaaagagtgg
    37441 cgggaagcgt ggcaaaaacg ggcttttgct cacatttcaa atggttataa acatatttat
    37501 atagcgatta attcaccaga gatatttctg ctggtttgct ccctcatcag aatttaacac
    37561 taaaagagca ggtaaaattg tctgaatgtt ctttaagtta ttcataaagc aaattaataa
    37621 atctgatgaa tatgttaacc ttcagcgaca tcatcggtga aaacctataa atgaagaagg
    37681 aaagcaaaaa aatgaagacc attctacctg aagtattgtt tgccgctttc gctaccactt
    37741 ccgcctgggc ggcagaaagc gtccagccac tggaaaaaat cgcgccttat ccacaagctg
    37801 aaacaggcat gaagcgtcag gtgattcagt taaccccgca agaagatgaa tctaccctga
    37861 aagtagaact gttaatcggt cagacgctgg aagtcgattg caatttgcat cgtctcggcg
    37921 ggaagctgga aagcaaaacg ctggaaggct ggggctatga ttattatgtc tttgataaag
    37981 ttagctcgcc ggtttccacg atgatggcct gcccggatgg caagaaagag aagaaatttg
    38041 tcaccgcgta tctgggcgat gctggaatgc tgcgttacaa cagcaagctg ccgatcgtgg
    38101 tgtatacgcc agacaatgta gatgtgaaat accgcgtctg gaaggcggaa gagaaaattg
    38161 acaacgcgga agttcgctaa actgttgtga agtacggcac cccgttggtt ggataaggcg
    38221 gttacgccgc atccaacatc taacgcccga gccggttgcc tgatgcgacg ctggcgcgtc
    38281 ttatcagtat acgccacatc attacaacgc aatatccgcc acttcttttt gaacgggttg
    38341 cggtttcaac tgcggtttcg gcgtactatc tgctgcttgc ggtttgtcgt atttcagccc
    38401 cagcacttcg ctggtgtact gcaactcgcg ttctgttgcc gctacatcac cgttcagctt
    38461 gcgtccataa gacggaacga tcgctttcaa cgtagcctgc cattgcgggc tggaaacgcg
    38521 atcgccaaat actttttcca gtagattcaa cataatcggc gcggcggttg acgcccctgg
    38581 cgatgccccc agcagcgccg caatggtccc ttgctggtca ctgaccactt cggtacccag
    38641 acgcagtacg ccacctttct cggcatcacg cttgataatc tgcacacgct gccccgcttg
    38701 ccacaaacgc cagtcctctt ttttcgcttg cggatagtac tctttcaacg cttcaaaacg
    38761 atcctcttca ctcaacatca cctgactcac cagatatttc accagatcga aattatccag
    38821 cccgacgtgc atcatcggca tcacgttaga ggtggtggtg gaactcatca gatcccacaa
    38881 tgaaccgttt ttgaggaatt tggttgagaa ggtggcaaat ggcccaaaca gcactacgcg
    38941 tttaccgtcc agaacacggg tatcgatatg cggaaccgac atcggtggtg cgccaacgga
    39001 tgctttaccg taaactttcg ccagatggcg attaaccacg tctggatttt ccgaaacaag
    39061 gaactgtccg cccaccggga aaccggcgta gtctttcgct tccggaatcc ccgattcctg
    39121 taacagcttc agcgccgcac cgcccgcgcc gataaagaca aatttggcgc ggatgttctg
    39181 tgcagtgccg tttttcagat cggcaacggt aacggtccag gtgttatcgt cattacgctt
    39241 tagggcgcgg acttcgctgc tgagttgcag cgagaagtta gatttcttct gtaaggaagc
    39301 aattaactgg cgggtgatct caccatagtt cacatcagtg ccaatttctg ttgcggatcg
    39361 cgcccttcca tcactaacgg tgcccactct ttgatctgcg cgtgatcttc agagtaacgc
    39421 ataccgcgaa acagcgagct ttgttgcaac gcggcgtaac gggcgcgcag gaaattaacg
    39481 ttatcctcgc cccagacaaa gctcatatgc ggaacggtat tgataaatga acgcggagta
    39541 cgcagcacgc cgcgctcgac ctggtgcgcc cagaactggc gggaaatctg aaatgcttcg
    39601 ttaatggcga ctgctttttc aatactgatg ctgccatcgg cgttttgcgg ggtgtagttc
    39661 agttccatca gtgcagaatg cccggttccg gcgttgttct agccgttcga actctcctgc
    39721 gcgcccccct ccaggcgctc caccatggtc atcgaccatt caggctccag ctcgcgtcaa
    39781 taggtcccca acgtggcgct cataatgccg ccgccaatca acagtacatc agtttcctgc
    39841 tcctcggacg ctttcgcttt tgccgccatc gaaacggcat taagccccac ggccatcgag
    39901 aagagcatgg cagtcacttt tttcatcttg ttaatgcctt acttttagtc gctttattgc
    39961 aggtgagatt tgcgcggcat caacggtaac acttaagtaa caacatttaa ataatgttta
    40021 taaattatat tctaatttta gaaacattat aatttcgttg attaattata gggttattag
    40081 aaatgaaggg atttttagca tattgacctg acggcagcag tccgccaggt cagcgtcgta
    40141 atattatgcc gtccgggcaa cggcatcacg cgaagcggca tcgcgctctt cgcccgtcag
    40201 ctcgctaagt tgcccattgc gcatttccag caggcggtcg gcgtggataa agtaatgatc
    40261 atcatgactg atagcgaaaa tagttttacc catctcctgc atcagcggca gcaacacctg
    40321 ataaaactca cgacggaagt gtggatcctg atccaccgcc cattcatcca gcaggataat
    40381 atcgcgttct tctgccagcg ccagcaacag cgccacgcgt tttttctgcc cttttgataa
    40441 cttcaggtta acaatacgcc cgttgcttaa ctcaagctta tgagccattt tcagctgcgc
    40501 cagccacttc tcaaccagtt gcgggttagc gggttgcccc tccggcccca gcagttgatc
    40561 aaacagccag acatcggtaa acactgccga aaacagtttg cgataatctt ccggttgttc
    40621 gccgctgaca ggtttgccat ccagcaagat ttcgccgctt tgtggctgat acaagcccgt
    40681 caacaacatc gccagcgtcg attttccgct accgttaccg ccaatcagaa acagcagctc
    40741 gccacgtttg atggtgagat taatcggacc aacggaaaac gcgttatcct gataagcaaa
    40801 cgtcacgtta cgcagctcca gcgtttgcca gttgggaaac gcctgcgggc gcggaaactc
    40861 agctttgaaa ggcgcgagcg cgaatttgtt cagcttgtta aacgccacct gcgccgtcag
    40921 cagcgtcggc aatgcgccaa ccgccgaaag cagcggcgta cgcaggaata acagcgtcaa
    40981 cgaataggtc gcagcaacgt tggtatcagc ccaaccgaga ctgttcgcca tccagaacac
    41041 caggccgata gcgcccagca tcatgatgtt tgaccagttc acggcactaa gatggaaggt
    41101 gtctgcgcga ataatatgat ggcgatactc ttgcgcatcg gggatgtaga ggttgttaaa
    41161 cacatactcg gcgcgttccc ggttcagagt cagctctttg cgcccttcaa gtacagtttg
    41221 aaaatccgtg tacagcttgt cttcggtttc acgtagggtc gccatatgtt tgtacacccg
    41281 cgccaccagc acaaaaccgc cccagatggt gatcgccatc cagatagccg ttaccagcaa
    41341 cattttgccc gacagcatcc acagatacgc cgatgaaccg atagtgagaa tgatcccctg
    41401 ccccagttcc ggcagacgca caaaagcaat ggtgatattg cgcacatcgc tggttaatcc
    41461 cgccagcaac gaggcgctac cgagttgttc aatgcgctcg acgtgggtat ccagaatccg
    41521 cttgataaat tcactacgca gtcggtaaac gaagtgatgc cccaaagtgg tgagcgccag
    41581 ttgcgatccg agagtgactg ccatcaacag cagcaataat cccagaaact ccggtaacac
    41641 cagcagactg gtatccgccg tttcgataag gcgctgattg ataaaagcaa ttaagccaat
    41701 gcctaatgcc gcactggcga ggcttagcgc catcacactg ataaatggcc agcgatactg
    41761 ccgccagaca agtacaagaa gttccatgca gaaaacccgg acaatgaatt acagcccgca
    41821 gtttaaacat cttcgcgcgc acagcaataa taattcttat ttttattctt ttttacctgc
    41881 ctgacggaat gtcaggttgt agcggcagtc ggtggtgagt ggatgaaacc ccgctttcaa
    41941 cggttgaata ccgtgataaa acagccgcga ttcaccgccc cataccacca catcgccatg
    42001 ttccaacaac aaacgtttga gcggatcatt acgtttcagg ccaccaaatt gaaaaatcgc
    42061 gggtaagccc agagaaacag aaacaattgg cgcgcgcaga tccggttcgt ctttatcctg
    42121 atgcagcgac agtttcgcgc caggagcgta gcggttgata agacaagcat ctggctggaa
    42181 atctggatag cccgccgccg tagccgcacg ttgacataaa tcatgaaagc tttgtggcat
    42241 agcgggccac ggtttatttg tttgcggatc aatgggcgaa tagagataac cttgccgatg
    42301 ggtcgtccag cccagatgcc cacagttggt catcgccacc gacatggtat atccgcctgg
    42361 agcgaccatc tggcgaaacg gcgactggct ggcaacgtca ttaatatcgc ggattagttg
    42421 ctccgcagca ttaaaagcaa aacgccgtaa aattaccgca ccagccgcca gtggctcttg
    42481 ccacggttca gcatcggcaa acagatccaa cattacctct cctcattttc agcttcgcgg
    42541 cgcagcagtt gcgctttacg cgacacgccc cagcggtaac cggaaagtgt gccatcacca
    42601 cggaccaccc gatgacaggg tattacgata gccagcttgt tggcggcaca ggcgctggca
    42661 acggcccgta ccgctttcgg tttgccgatg gcgttagcca gttgctgata actgacggtt
    42721 tcaccacaag gtatcgtgcg cagtgcctgc cagacttgtt gctgaaaagc agtgccgcga
    42781 atatccagcg gtaacgtcag cggcgtatcg cgttgattga ggctggcgat cacttcacgc
    42841 acatgttgct gaaacgtcag atcggcaggc gcgttgtcgg cagcgggaaa catctgctgc
    42901 aactcgctga ttaatgtcgc gtcatcatcg cccagcaata tcgcgcaaat cccccgctcg
    42961 ctttctgcca ccaggcaacg acccagctca caatcagcca gcgcgtaacg caccgccaga
    43021 ttttcgccac cgtgacggaa ttgtttagcc gtcatgccca gcgtttcatc ggctttgcgg
    43081 tagtaactgc tgctgtcggg gaatccggcg ttaagaatag acgtcgtcac gctctcccct
    43141 ttcgccagcg attcgcgcaa acggcgagcg cgccaggcct gttgccaggc tttaggcgtc
    43201 attccggtag tagctttaaa caaccgatgt agatgaaatg gactcatcgc cacctggtcg
    43261 gctaaggctt ccagcgttac aggcgtttcc tgttccagca gtcgacacgc gtgggtgatt
    43321 ttatccaacc gatgttgccg gggattggct ttgtctggct gacaacgttt gcaggggcga
    43381 aatccggcgg cgagtgcctc gctggcattt gcgtagaagg agacattttc ccgcaaggca
    43441 tgtctggcgc ggcaagaagg acggcaaaag atacccgtgg tgcgcacggc gaaaacgaat
    43501 tcgccgtcgg cattcgggtc gcgggctaag acagattgcc agcgttgatc gtcagttaag
    43561 catgtggcgt ttttcataat cagctccctg gttaaggata gcctttagtc tgcccggtca
    43621 ccatcacgca aaaaccaaca atcttgcgct ttaatttttt tcgctgacaa ggaagctttt
    43681 aaactgtggt gacatccagg ttttaaagct atcgccttct ttggtgatca tataaaccgc
    43741 cagcccttcc cggcgaacaa cctctttggc tttctccggc ccgagtacca tcaagccagt
    43801 atcccaggca tcggcttcca gcgccgtcgg agcaatcacc gtcacggata ccagattgtg
    43861 ttcgatggga cgcccggttt gcggatcgat aacatgggaa agacgtttgc cgtccagttc
    43921 gtaatagtta cggtagctgc cagaggtgct aatcccatga ccgttgatat ccaccacagc
    43981 ctgaaccgcg ttttctttat cggttggttt ttgaatcgct acccgccacg gctggccttc
    44041 accgttcata ccacggctgt tcagcgcgcc gcccaccgac accagatagc gggaaatccc
    44101 ttcctgctcc atcaagcgtg ccaggtgatc cgccgcataa ccttcaccga cggtggagag
    44161 atcgacatat aaatccggca ggtctttttg cagatattgc tgatgcgact gattgatgac
    44221 cgtcaggtgc tgtaagccgg ttttggcttt catcgcatcg atctgttcct ggctcggaat
    44281 ttgaaccggc tgttgttccg ggccaaagcc ccacagattc accagcggcc cgacggtaat
    44341 atccatcgca ccatcggtcc ttgccccaat gcgcagcgag gtggtgacga tatcggccat
    44401 cgcttcactt accggccacg gcgacaaact ttgcgagtcg ttaaagcgca tcagcgcgga
    44461 atctttttta taggtcgaaa gcagctgatc gtcggcgtcc agctgggtct gaatcttttc
    44521 tttaagttcg gcactgcgtt tggcatcaat gcccgggatg ctggcacgcc agaaggtacc
    44581 catggttttg ccttcaagaa cggtaacttc agtagcgtgg gttttggcgg gttgtggttt
    44641 ttgatcgcaa ccaacaaaga agagcgcggc aaccagcagt gccacgcggg taaagcttat
    44701 ttccatacgt gattatcctc atgcgaacgg tcgcaagagt acaccaaaaa actgtgtttg
    44761 tacgctgaaa acaatgaaaa aagggcccgc aggccctttg ttcgatatcc atcgagatta
    44821 gaactggtaa accagaccca gagctacgat gttatcagtg ttgatgccag cgtcacgagt
    44881 gaactggttg tcgtccagca ggttgatttt gtagtcaacg taggtggaca tgtttttgtt
    44941 gaagtagtag gtcgcaccaa catcaacata tttcaggata tcttcgtcgt cgtagttacg
    45001 accattgatg acacccaggt ttttaccttt agactgcagg taagccaggg acggacgcag
    45061 accgaagtcg aactggtact gagcaacagc ttcgaagttc tgtgctttgt tcgcccaacc
    45121 caagttgcct acgcgagttg cgttgtaggt ctgggtgtac tgagcagcta ggtagatgtt
    45181 gttcgcgtcg tatttcagac caccggtgta ggtttcagcg cggtcgccat taccgatgta
    45241 acgctcatca taacggttca gaccgaagtt ctggtcatca gtacgtttgg agctggaaac
    45301 tgcagcaccg ataccgaagc cttcgtaatc ataagtaata gaaccgccaa cgccgtcacc
    45361 gttctgacgc agagcttcac gaccgttgtt ggtcatgcct tcaccttccg ggctaccgtt
    45421 tttgccctga tactgaacag cgaagttcag accgtcaacc agaccaaaga agtcggtgct
    45481 acggtaggtt gcgaagccgt taccacgctg ctgcatgaag ttgtcagaac cgtaagtgtc
    45541 accaccgaat tctggcagta cgtcggtcca ggaagttacg tcgtaaacta cgccgtagtt
    45601 acgaccgtag tcgaaagaac ctacatcctg gaatttcaga cctgcgaatg ccacacgggt
    45661 ccaggagttg ttttcgtttt cagcgctgtt gccctggatc tgatattccc actggccgta
    45721 accggtcagc tggtcagtaa cctgagtttc acctttgaag ccaagacgca tgtaggtctg
    45781 gtcgccatct acagacttgt cgtcagagaa atagtgcagg ccgtctactt taccgtacag
    45841 atctaatttg ttgccgtctt tgttgtaaac ttcagcagcg tttgctgcgc ctgctaccag
    45901 cagagctggg accaggaggg acagtacttt aactttcatg ttattaaccc tctgttatat
    45961 gcctttattt gcttttttat gccactgcat actgattaac cctcattaat cagtcggcaa
    46021 gtccattctc cccaaaaatg cagaataatc caacacgaat atgatactaa aacttttaag
    46081 atgtttcatt tatcgctata gatgtttcaa aatgtaaata caagggaact ttttaagatt
    46141 attgcggaat ggcgaaataa gcacctaaca tcaagcaata ataattcaag gttaaaatca
    46201 ataacttatt cttaagtatt tgacagcact gaatgtcaaa acaaaacctt cactcgcaac
    46261 tagaataact cccgctatca tcattaactt tatttattac cgtcattcat ttctgaatgt
    46321 ctgtttaccc ctatttcaac cggatgcctc gcattcggtt tttttaccct tctttacaca
    46381 cttttcatta ttctgtgcta ccacagaaaa actataacgc ttgttaacta tttcacaaat
    46441 aattaacatc agcataattt ccagcaatct ttgtttattt gcaattaatt tcgttgggct
    46501 ttttgtaggt tatttgtaca gcaaaatggc gcttgtacat ctatttcccc ccatacagga
    46561 tgataaatat cacgggagaa tagagaatca tcaatcaggt aagagtctgg aatttcacac
    46621 tgtacccttt atactgccct atcacttcgc gaagttttaa caggtcataa acacgaatgc
    46681 gtcagaaaga gacaacggcc acgacccgct tttcactcct accggggagc attacccgct
    46741 tctttttact gttgatcatt gtgttactgg tgacgatggg ggtaatggta caaagtgccg
    46801 ttaacgcttg gctgaaagat aaaagttacc agattgtcga cattacccac gcaatccaaa
    46861 agcgcgtcga tacctggcgt tacgtgacca ggcagatcta cgacaacatt gccgcgacga
    46921 cctccccctc ctccggcgaa ggtttacaag agacgcgcct gaaacaggat gtctactatc
    46981 tggagaaacc acgccgcaaa acggaagcgt taatctttgg ctctcacgac aactcaacgc
    47041 ttgagatgac tcagcggatg tccacttatc tggatacatt gtggggtgca gaaaatgtac
    47101 cgtggtcgat gtattacctg aatggtcagg ataacagtct ggtgctgatc tcaaccctac
    47161 cccttaaaga tctcacctcc ggatttaaag aatcgaccgt cagtgacatt gttgattcac
    47221 gtcgtgcaga aatgttacaa caggccaacg ccctcgatga acgcgaaagt ttttctaaca
    47281 tgcgccgcct agcctggcag aacggtcatt actttacctt gcgtaccacg ttcaaccagc
    47341 cgggacatct ggcaacggtc gtggcttttg atctgccgat taatgatttg atcccaccgg
    47401 gtatgccgct ggacagtttc cgccttgagc cagacgcgac ggcaacggga aacaatgata
    47461 atgagaaaga agggacggat agcgtcagta tccactttaa cagtacgaag attgaaatct
    47521 cctcggcact caactctacc gatatgcgcc tggtctggca ggttccttat ggcaccttat
    47581 tgctggatac gttgcaaaac attctgctgc cactgctgct gaacatcggt ttgctggcgc
    47641 tggcgttatt tggctatacc acattccgcc atttctccag ccgcagtaca gaaagcgtcc
    47701 ccagcacggc ggtcaataac gaattgcgca ttttacgggc aatcaatgaa gagatagtct
    47761 cactgttgcc gctcggcctg ctggttcacg atcaggaatc gaaccgcact gtcataagta
    47821 acaaaattgc cgatcatttg ctgccgcatc tgaatctgca aaacatcacc accatggcgg
    47881 aacagcatca ggggattatt caggcgacga tcaataacga gctgtatgag atccgcatgt
    47941 tccgcagcca ggtcgcgccg cgcacacaaa ttttcattat tcgcgatcag gatcgcgaag
    48001 tgctggtaaa caagaaactc aagcaggcgc agcgtctgta tgagaaaaac cagcaggggc
    48061 ggatgacctt tatgaaaaac attggcgatg cgctgaaaga acccgcacag tccctggcgg
    48121 agagtgcggc taaactcaac gccccggaaa gcaaacaact ggcgaatcag gcagatgtgc
    48181 tggtgcggct ggtcgaagaa atacagttag cgaacatgct tgcggatgat agctggaaaa
    48241 gtgagacggt gctgttctcc gtgcaggatt taattgatga agttgtgcct tcagtgttgc
    48301 ctgccatcaa gcgtaaaggt ctgcaactgc tgattaacaa tcatctgaaa gcacacgata
    48361 tgcgccgcgg cgatcgcgat gccttacgac gtattttgct gctactgatg caatatgccg
    48421 tgacctcaac gcaattggga aaaatcaccc ttgaggttga tcaggatgag tcctctgaag
    48481 accgcctgac attccgcatt ctggacacgg gagaaggcgt aagtattcat gaaatggata
    48541 atttgcactt cccgtttatc aaccagaccc aaaacgatcg ttatggaaag gcggacccgc
    48601 tggcattctg gctgagcgat caactggcac gtaaactggg cggtcattta aacatcaaaa
    48661 cgcgggatgg gcttggtaca cgctactctg tgcatatcaa aatgctcgcc gctgacccgg
    48721 aagttgaaga ggaagaagag cgtttactgg atgatgtctg cgtaatggtg gatgttactt
    48781 cggcagaaat tcggaatatt gtcactcgcc tgttagaaaa ttggggtgca acctgtatca
    48841 cacccgatga aagattaatt agtcaagatt atgatatctt tttaacggat aatccgtcta
    48901 atcttactgc ctctggcttg cttttaagcg atgatgagtc tggcgtacga gaaattgggc
    48961 ctggtcaatt gtgcgtcaac ttcaatatga gcaacgctat gcaggaagcg gtcttacaat
    49021 taattgaagt gcaactggca caggaagagg tgacagaatc gcctctgggc ggagatgaaa
    49081 atgcgcaact ccatgccagc ggctattatg cgctctttgt agacacagta ccggatgatg
    49141 ttaagaggct gtatactgaa gcagcaacca gtgactttgc tgcgttagcc caaacggctc
    49201 atcgtcttaa aggcgtattt gccatgctaa atctggtacc cggcaagcag ttatgtgaaa
    49261 cgctggaaca tctgattcgt gagaaggatg ttccaggaat agaaaaatac atcagcgaca
    49321 ttgacagtta tgtcaagagc ttgctgtagc aaggtagcct attacatgaa caatatgaac
    49381 gtaattattg ccgatgacca tccgatagtc ttgttcggta ttcgcaaatc acttgagcaa
    49441 attgagtggg tgaatgttgt cggcgaattt gaagactcta cagcactgat caacaacctg
    49501 ccgaaactgg atgcgcatgt gttgattacc gatctctcca tgcctggcga taagtacggc
    49561 gatggcatta ccttaatcaa gtacatcaag cgccatttcc caagcctgtc gatcattgtt
    49621 ctgactatga acaacaaccc ggcgattctt agtgcggtat tggatctgga tatcgaaggg
    49681 atcgtgctga aacaaggtgc accgaccgat ctgccgaaag ctctcgccgc gctgcagaaa
    49741 gggaagaaat ttaccccgga aagcgtttct cgcctgttgg aaaaaatcag tgctggtggt
    49801 tacggtgaca agcgtctctc gccaaaagag agtgaagttc tgcgcctgtt tgcggaaggc
    49861 ttcctagtga ccgagatcgc taaaaagctg aaccgcagta ttaaaaccat cagtagccag
    49921 aagaaatctg cgatgatgaa gctgggcgtc gagaacgata tcgccctgct gaattatctc
    49981 tcttctgtga ccttaagtcc ggcagataaa gactaatcat ctgtaggcca gataagacgc
    50041 gttagtgtct tatctggcat ttgcaccgat tgccggatgc ggcgtaaacg ccttatccgg
    50101 cctacgattc ccattatttc aacagattac attaaagtag gccagataag acgcgtcagt
    50161 gtcttatctg gcatttgcac tgaatgccgg atgcggcgta aacgccttat ccgtcctacg
    50221 aatcccgcga tttcctgacc ctctcggcat ataccgtcag cgtctgtttt atcacatcca
    50281 gcgttaccgg cttcgagagg cagctgtcca taccggactc cagacaccgc tgcttctctt
    50341 cagccaacgc attagcagtt actccgatta ccggcaacgt cagtcccaac tgacgaatgc
    50401 gttgcgtcaa gcggtaacca tccatatttg gcatgttgac gtcgctaagc acgatatcaa
    50461 tatgattctt actaagtaca ttaagcgcat cgacgccatc attcgcggtt ttacattgat
    50521 agcccaacga tcccaactga tctgccagca gacgtcggtt aatcggatga tcatccacga
    50581 ccagaatcat catatcgtcg ttatcgctga ccgctttgtc cgtcgacggc agagcgttag
    50641 caggatcgtc gctctccatc tcgatcaaat aaatacgcgc caacaatgcc ggtagctcat
    50701 gcggagcagc cacactgtgt acccactccc ctggcgcttt ctccagcgga ataccaatat
    50761 ggcgacgaca gaaggtcact accgctctgc cctgccattt tttactcact acctcgtcag
    50821 tgattaacac atcttcggga gtcggttcct gcccttcgta tgttgtaacg acgatgccgc
    50881 tgcgctgcaa actggtttcc aggaactgac agagcgacgc attgcggacc gccagccagc
    50941 agcgtttacc actcaaccct tccacgcctt ttttctgcgg gtactgagcg ccgtacaacg
    51001 gaatacgcac ggtaaactgg ctgcccattc ccggttctga atctaccgag atatcgccgt
    51061 ccatcatgct gatcagtttt tcacaaatcg ccagacccag accggtcccc tggaagtttc
    51121 gctgtacgcc cgttccaacc tggaagaagg gatcaaacaa gcgcaccact tctttcgccg
    51181 gaatccccac gccggtatcg cgaacacgga tagagagata atcgccatca gcgcgaacat
    51241 gcaaaactat acagccggta tcggtgaatt ttatggcgtt actcaacagg ttggagatga
    51301 cctgctgtaa acgcatcggg tcgccattta aggccactgg cacatccggt tcaataaagc
    51361 agtacaagcc taactgcttg cgtaccacca gcggtaaata gttggcggtg atgtggttca
    51421 tcacttcacg cggtgaaaac tcacgcggtt cgatcttcaa ctgttccgat tcaatcttcg
    51481 agaaatcgag aatatcgctg ataattttca acaacaggct ggaagagttg ttcattgccg
    51541 tcaccagacg atcgacgcct ttcggtaact ctttggtttg caacagatcc aggttaccga
    51601 taatgccata cagcggcgtt cgcagctcat gactgacggt ggcaaggaac atcgattttg
    51661 actggctcgc ctgttccgct gcttgtgcca tctcctgcaa cgactcttcc atcttcacgc
    51721 gcgaagaaac atccaccagc acacaaatgg ccacgttttc attacgatag cgcgaatgga
    51781 cgaagctgat ttgcagattg gtattgttgc tggtcaggac atcaacaaaa ttgacctgct
    51841 gcccacagat aatttgcgtc agtcgttggc ggtcctcatg cgtaagcata ttgagatagg
    51901 tatgcgccag ttcgttactt aaaatattga cgccatcagc ggtacgcaaa atgcagatac
    51961 ccactggcgc ggaggcgaca atcttgcgat tgaactgctc atgttcttcc agtcgcaggg
    52021 cgtcgctttc cgccggaatg aaaatacgtc gctcgtacat ccgcgcgaga gtaaacaatg
    52081 cagctccggc aagcacattc agcaaaattg cgttaaggat caacatgcga atgcgttcca
    52141 gcaccttatc aaccggcacc gaatacacga tgcttagcga tgagggtggc agatttttct
    52201 tcagcaccag ctcccggaac ccttccgtat agccaaacca ggagcgttcc tgcatccagc
    52261 gaggatcgcc cttaatttta ctttctggtc cggtaagcga aatcagggta tgaccatttt
    52321 catcaagaat ggtaaccccc atcggcaacg tacccggtaa gaaaaagttc tccatccgga
    52381 tggtctgctc gacacccaaa agcgcctgca accggttcgc cagataaact ggcgtcaacg
    52441 cgtaaaaata cccgacgcaa gggcgcggac cttcgctgat ccagtagagg ttactgccgc
    52501 tatcatcttg tggtgcattt cgatatttat tgatgcgttc atgcaacgct ttcaacgcgg
    52561 tatcgcgttc cactggcata tcacgcagac cgaaattggc catgcagagg ttatcactgc
    52621 cgattaaaaa tacccggttg agatcgtaag ccgcagaaaa attatcgcgc cagtagcgca
    52681 taaaccacgc caatgactcc agagaacctc gccaggtgtt actcattgcg gaacaatcgg
    52741 agtcggcaaa cagcggttca aacgcaggca catccgcctg cgtttctcgt ccacgcgggg
    52801 aaagcacacc gttttctgcc gataagcgat tttcggcgat gtacttaagc tctttcatca
    52861 catcagaggt gcgttgaata aagcgctgag cctgatcgga actcagatta aattcctgac
    52921 gaatttccga ttctcgctga tgtaacgcat taacgatgta aaaaacggat gaaaaagcaa
    52981 tcaacagcca gagcactaac gccaatgctc tgaacatgta gcgcgaggct ttcagggttg
    53041 tacgaaaaga agcaaggtat ttcaaagggg agaagctccg cctcaggtga ccgatggagt
    53101 gtggttaagg tagcggtaaa agcgtgttac cgcaatgttc tgtcaccatt gcaataatgc
    53161 gtatcgctta gaaaccattg ccttaaccag gaaagccgga gtagatggac agttctcagt
    53221 acgaaaaata agaaatgacc aatctgccga cgcttgttaa tggttatatc ctggcgtcgg
    53281 cagatcgccc agcagtctta tctgaaaaaa gagagagcca ggttgtgtga agggatggat
    53341 caccagtcaa aacttttcac cagcgtcagc tcgccagcat tacgcatcgg tacaataaat
    53401 gtttcctgtt tctcattgac cgatccttca tcggtgatca gcgtcagttg ggcggtcgtc
    53461 aattccgttt cgctgcgccc accatagtag ttgatataca cctgatagcg cccgtgaact
    53521 ggtgctggca tggcgaaaat ctcgggtccg taccccgtcg tgacatccat atccagtgca
    53581 ccactgtttt tcagcacggt gttaccgtac caggcgtgtt cgccatcggg cgtgacaaca
    53641 tggagatcga gatcggtatt gtccgtatcc cacgagagaa ccagccgtaa acgtgcacga
    53701 atagttcctg tacccggcgt tgagtaaaac tgcatttttt gccggctttg cccatccggg
    53761 ctgatgacct gcacgctgtt gctgccttca gtgaaaatat aagggcgtgc aaatgaacca
    53821 tcggattcaa ttctctgcgg catactggca ccgttgacca ccagccggcc ctgctgaact
    53881 ttacccgccg cagcataatt ctttattttg ccgcggatct gcgctgagat attttgatca
    53941 tctgccatgt tgacggacga cgccgggtaa ttgatcgatt gtgaaaaact ggcatcttcg
    54001 ccctctgccg gatgccagcc agagagcggt gcgtcaatct cgacttcctg cacaccttca
    54061 ctgtgagcga caggcgaaag tgccaccagt aacaacggaa gaaaaatctt tcgcatagca
    54121 ttagtccatt aataattgtc gggtcagcgt ttcgatatag ttctcatcaa gcccggtggg
    54181 ataaccatca aaggccaagt gcagatactc atgggttaaa tccagccgat cctgaagcgt
    54241 aaagaagtta cgaataaaca gccgtttctg ctgccgatcg gtatagggga aacctgagac
    54301 taaacgacag accgcaaaca cgtctggttc gttgtacccc gtctcgcctt gtaatatacg
    54361 ccgccactgc ggcatttttt tcgccagcca ggctttcgct ttgggtaata actggcaggt
    54421 tgagcgtgga gctccccagc ggctaaggct gttgtcggga taagcaaacg cgagtatctg
    54481 gtcgtaacgc tccccctgcc ccgcctgggc cgtggcttgc cgccaggaaa gtgtcccttc
    54541 ggtagcgcga ctgccatgat aatgaaccgg atcgcctgca tagatgagct cctgcgtcca
    54601 ggcggtcatc gttcgcgccc ccgtggtcgc tggcgaagca gaaacacgct gcgtggcgct
    54661 gctgtcgggg atggtcaggc aatcaccttc ccgattggcg ttttgctgta aaaaagtacg
    54721 tatcgccaca gtcatggctt tcgctgcctc aggtggcgta cttttcgcct cgcgatccag
    54781 aactctggca acgtactctt cacgatcgag atgcgactgc aatttcagtt tgcccttttc
    54841 gctcagtaag gtcgtctcac cgtggctgac aaacgtaatg tggttaccat tagtaaacgt
    54901 cacacgatag cgcccattaa gtacgctggg ttttactgcc gtcgtactct tttccgctgt
    54961 gattttcttc agcggatagc gggcaaacag ctccacctct acacattgcc cactggcaac
    55021 ctgcgtcggc actggcaaaa cccgattaag caccgtcgca taacgggtta gtaccgtttt
    55081 gctggtgcca ctgccagtaa cccacagcgg tgtaccatcg gttaaccagc cagcaaaacc
    55141 accctgacgg atttcctgtt tatcatcggc aaaccagctc caggttttca cccgtacccg
    55201 actaccgagc atggatgcga caccgatttt cgcctcgtcc agcaccacat ccagtaagac
    55261 ttcctgagct ttgttttgcg caggtaacgt cgctaatgaa tcaagcagcg atttaaccgt
    55321 taccgaggtt tctggcttca gcgttgtaag agacgctaac cacgcaggtg cctgacgctg
    55381 ttgccagtat tgcccccaca catctgcgcc aatatgcaac cgctgcggcg caaaatagag
    55441 cccacaggaa cgtaccagcg cggtatcgcg cgttatgctc tccccagcct gacaacaata
    55501 aacctcttcc ggggagtttc cgcgacactg gtagggctgc tccggttgat gggtatcctc
    55561 cagccaggcg taaacataca gtttccacaa actccctaac ggcgtggtga gcgagtcagg
    55621 taatgctgag actttggtca cgcctgatga cgacagctga tacagttgat cgtgctgcgc
    55681 accgcgcagc accagttgta acggcgtctc ctccgcaagc gttggcagag taaccaacgc
    55741 caatagccag acgattctgc gccagttcac ttatttcacc tgcatcccgg tccattcact
    55801 ccctgccgcg acactttgct gcgcaggtgc ataggaacgc acataacgag caggaggcag
    55861 aacgaattgc cctttttgcg agaagcgcag caaatggcgg aaagtgaccg ttcccgtcag
    55921 ttctttcacc ggcaccatat aggccagttc gcccatttca ttacgcgctt tttccagcaa
    55981 ttgcccctgc tgtttcgcgg cgttgggttt attgactgaa atgccccatg ttgtgcgctc
    56041 aacgtcagct cccggcggga gcggtacttc cacctgacca tagcgcagaa ctgcatcctg
    56101 ctcgctggta agcgtaattt catcgagata cagcgcatcg ctgtcaatct cattgctggt
    56161 caccggctgc agagtaaagc tcatctcttc ttcaccgggg ataagccgat acaactggcg
    56221 ttcaacggtc accggaatgt tactttgttg agccgttttt gccggttcac gccagcggac
    56281 ctgcacattt gcggcgataa ttcgtcacca aaagagagaa tgtccggcac accctaacca
    56341 acccaacgcc agtcttcgcc ccctccagtt aacttatgtt tagcccatgc gcccgcaggc
    56401 gcaggcaaaa caactggagg cattgtcgcc atatatttcg ccagccagtt catggccagc
    56461 gcgcgctcaa tagtggattg ctcagcggtc aaaccattaa aatagcggcc gtctgggtag
    56521 cgtcaccacc gctattgagc agcacaaccg tgtgcgccag cggctgattg ctgtttgccg
    56581 ctttatccca cgcatattgc tgaacctgtg gcatcaccgt ggacttcaac tgcgcttttt
    56641 tcagcaacgt catggttaag acgtttgcca ccgcatctgc cagtggagat tccggtgtat
    56701 cgaggatcag gctgtcattg atatcgcgga tgtcttcctc agagaaatct tcatctttag
    56761 ttccgcgccg ggcgatagct tcatcccccc ctttcaacaa cgttttgcac ggcagattca
    56821 tctcctgtgc ccacgccagc accagcgccc gatgcaataa cggcatatta tccgcctgct
    56881 cggcataact gtcgagcata tgctgccagt attccggttg ttgcgttacg ccgagcgcct
    56941 ggctggcctg ccagtcggcg taccatgccc atgccgtaag gaaggcgtca ccattgccat
    57001 cttcacccca ccaggtaaag cgcgctccgg gccccgccag ttgcatcagc cgcagacggt
    57061 tatcctgaat catctgacga atgtcgttag cggcggcact ttgatgatcg gcaagcgaac
    57121 gccaggcgag actgagcggg atcagacggc taccggtgtt gatcacccca ccccacggtt
    57181 catcgactaa cgcatcaaga ttgttgcgga aaatctcctg cggcgtttca ctactttgta
    57241 gccggatatt actcgcctgc tcgggcaaca tcagcgcgtt ctcgccaccg ccgagcatga
    57301 catttttctg ctgttcaacg ggccagctgt tgtccacaaa agacagtttt gtgctaatgc
    57361 tgtcctgcac ttgcccattt tgttgcagtt ctgcacttaa caagccagat tgctgaatat
    57421 tctgcgtcag cgaaatataa ttcgcccctt tgtgcagcgt cagcgtctgg cgcatctcag
    57481 cgcctgcaaa tttagtcacc agcgccaccg gttcgttatc ctgctgactg aagataaaca
    57541 gtcctgccgc cggtttgtcg cccacgcgat acaccgttgg catactccac ttcatgtaga
    57601 gatttttttc cgaacgcaga taagcacgcc cctgcccgac cagcccgtcg ccgttcatcc
    57661 cacgcgcggt gatacgccag cgggttaacg aatcaggcat caggaacgtg aaatacgctt
    57721 tgccttgttt atcggttgtg agtgacggca tccatgccgc ggtatccacc tcttcacgcc
    57781 gtggacgttc aagcattttt actcgccgct cgctgcggtt agtcgcgcca ggcgcaaccg
    57841 gctcgctgga gagcgcctgg tcatagctga taaacgacag actggagctg gtacgcacat
    57901 tgttacgccc cagcggatag aaaaatttgc cgatattcgg
//
LOCUS       DAJTQQ010000015        55636 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000015 DAJTQQ010000000
VERSION     DAJTQQ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.015"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(1..435)
                     /locus_tag="OO309_001002"
                     /pseudo
     CDS             complement(1..435)
                     /locus_tag="OO309_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(410..1210)
                     /gene="otsB"
                     /locus_tag="OO309_001003"
     CDS             complement(410..1210)
                     /gene="otsB"
                     /locus_tag="OO309_001003"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR5846238.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(1377..2363)
                     /gene="araH"
                     /locus_tag="OO309_001004"
     CDS             complement(1377..2363)
                     /gene="araH"
                     /locus_tag="OO309_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR5846239.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2378..3892)
                     /gene="araG"
                     /locus_tag="OO309_001005"
     CDS             complement(2378..3892)
                     /gene="araG"
                     /locus_tag="OO309_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR5846240.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(3962..4951)
                     /gene="araF"
                     /locus_tag="OO309_001006"
                     /pseudo
     CDS             complement(3962..4951)
                     /gene="araF"
                     /locus_tag="OO309_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            5748..6251
                     /locus_tag="OO309_001007"
     CDS             5748..6251
                     /locus_tag="OO309_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR5846241.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6331..6582)
                     /locus_tag="OO309_001008"
     CDS             complement(6331..6582)
                     /locus_tag="OO309_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR5846242.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6697..6783)
                     /gene="azuC"
                     /locus_tag="OO309_001009"
     CDS             complement(6697..6783)
                     /gene="azuC"
                     /locus_tag="OO309_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR5846243.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7046..7369
                     /locus_tag="OO309_001010"
     CDS             7046..7369
                     /locus_tag="OO309_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR5846244.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7540..8037
                     /gene="ftnA"
                     /locus_tag="OO309_001011"
     CDS             7540..8037
                     /gene="ftnA"
                     /locus_tag="OO309_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR5846245.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8074..8313)
                     /locus_tag="OO309_001012"
     CDS             complement(8074..8313)
                     /locus_tag="OO309_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR5846246.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            8503..9714
                     /gene="tyrP"
                     /locus_tag="OO309_001013"
     CDS             8503..9714
                     /gene="tyrP"
                     /locus_tag="OO309_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR5846247.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(9776..10441)
                     /gene="yecA"
                     /locus_tag="OO309_001014"
     CDS             complement(9776..10441)
                     /gene="yecA"
                     /locus_tag="OO309_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR5846248.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10637..10723)
                     /locus_tag="OO309_001015"
     tRNA            complement(10637..10723)
                     /locus_tag="OO309_001015"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10687..10689),aa:Leu,seq:taa)
     gene            complement(10736..10809)
                     /locus_tag="OO309_001016"
     tRNA            complement(10736..10809)
                     /locus_tag="OO309_001016"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10775..10777),aa:Cys,seq:gca)
     gene            complement(10864..10939)
                     /locus_tag="OO309_001017"
     tRNA            complement(10864..10939)
                     /locus_tag="OO309_001017"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10904..10906),aa:Gly,seq:gcc)
     gene            complement(11091..11639)
                     /gene="pgsA"
                     /locus_tag="OO309_001018"
     CDS             complement(11091..11639)
                     /gene="pgsA"
                     /locus_tag="OO309_001018"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR5846249.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(11696..13528)
                     /gene="uvrC"
                     /locus_tag="OO309_001019"
     CDS             complement(11696..13528)
                     /gene="uvrC"
                     /locus_tag="OO309_001019"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR5846250.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13525..14181)
                     /gene="uvrY"
                     /locus_tag="OO309_001020"
     CDS             complement(13525..14181)
                     /gene="uvrY"
                     /locus_tag="OO309_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR5846251.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14477..14653
                     /locus_tag="OO309_001021"
     CDS             14477..14653
                     /locus_tag="OO309_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846252.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            14640..14864
                     /locus_tag="OO309_001022"
     CDS             14640..14864
                     /locus_tag="OO309_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR5846253.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(14897..15517)
                     /gene="sdiA"
                     /locus_tag="OO309_001023"
                     /pseudo
     CDS             complement(14897..15517)
                     /gene="sdiA"
                     /locus_tag="OO309_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(15747..16499)
                     /gene="yecC"
                     /locus_tag="OO309_001024"
     CDS             complement(15747..16499)
                     /gene="yecC"
                     /locus_tag="OO309_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR5846254.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(16496..17164)
                     /gene="tcyL"
                     /locus_tag="OO309_001025"
     CDS             complement(16496..17164)
                     /gene="tcyL"
                     /locus_tag="OO309_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR5846255.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(17179..18165)
                     /gene="dcyD"
                     /locus_tag="OO309_001026"
     CDS             complement(17179..18165)
                     /gene="dcyD"
                     /locus_tag="OO309_001026"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR5846256.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(18270..19070)
                     /gene="tcyJ"
                     /locus_tag="OO309_001027"
     CDS             complement(18270..19070)
                     /gene="tcyJ"
                     /locus_tag="OO309_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5846257.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(19158..19709)
                     /gene="fliZ"
                     /locus_tag="OO309_001028"
     CDS             complement(19158..19709)
                     /gene="fliZ"
                     /locus_tag="OO309_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR5846258.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(19755..20474)
                     /gene="fliA"
                     /locus_tag="OO309_001029"
                     /pseudo
     CDS             complement(19755..20474)
                     /gene="fliA"
                     /locus_tag="OO309_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(20639..22291)
                     /gene="fliC"
                     /locus_tag="OO309_001030"
     CDS             complement(20639..22291)
                     /gene="fliC"
                     /locus_tag="OO309_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR5846259.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            22557..23963
                     /gene="fliD"
                     /locus_tag="OO309_001031"
     CDS             22557..23963
                     /gene="fliD"
                     /locus_tag="OO309_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR5846260.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNIAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            23988..24398
                     /gene="fliS"
                     /locus_tag="OO309_001032"
     CDS             23988..24398
                     /gene="fliS"
                     /locus_tag="OO309_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR5846261.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            24398..24763
                     /gene="fliT"
                     /locus_tag="OO309_001033"
     CDS             24398..24763
                     /gene="fliT"
                     /locus_tag="OO309_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR5846262.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            24841..26328
                     /gene="amyA"
                     /locus_tag="OO309_001034"
     CDS             24841..26328
                     /gene="amyA"
                     /locus_tag="OO309_001034"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5846263.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(26362..26775)
                     /locus_tag="OO309_001035"
     CDS             complement(26362..26775)
                     /locus_tag="OO309_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5846264.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            26962..28167
                     /gene="yedE"
                     /locus_tag="OO309_001036"
     CDS             26962..28167
                     /gene="yedE"
                     /locus_tag="OO309_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR5846265.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            28164..28397
                     /gene="yedF"
                     /locus_tag="OO309_001037"
     CDS             28164..28397
                     /gene="yedF"
                     /locus_tag="OO309_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR5846266.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            28504..29174
                     /locus_tag="OO309_001038"
                     /pseudo
     CDS             28504..29174
                     /locus_tag="OO309_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(29214..30422)
                     /locus_tag="OO309_001039"
                     /pseudo
     CDS             complement(29214..30422)
                     /locus_tag="OO309_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            30490..31105
                     /gene="tnpA"
                     /locus_tag="OO309_001040"
                     /pseudo
     CDS             30490..31105
                     /gene="tnpA"
                     /locus_tag="OO309_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            31433..32515
                     /locus_tag="OO309_001041"
     CDS             31433..32515
                     /locus_tag="OO309_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCR5846267.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(33004..33802)
                     /locus_tag="OO309_001042"
                     /pseudo
     CDS             complement(33004..33802)
                     /locus_tag="OO309_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(33812..34363)
                     /gene="ybcL"
                     /locus_tag="OO309_001043"
     CDS             complement(33812..34363)
                     /gene="ybcL"
                     /locus_tag="OO309_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR5846268.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(34532..34864)
                     /gene="emrE"
                     /locus_tag="OO309_001044"
     CDS             complement(34532..34864)
                     /gene="emrE"
                     /locus_tag="OO309_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR5846269.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(35209..35523)
                     /gene="fliE"
                     /locus_tag="OO309_001045"
     CDS             complement(35209..35523)
                     /gene="fliE"
                     /locus_tag="OO309_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR5846270.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            35737..37396
                     /gene="fliF"
                     /locus_tag="OO309_001046"
                     /pseudo
     CDS             35737..37396
                     /gene="fliF"
                     /locus_tag="OO309_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            37389..38384
                     /gene="fliG"
                     /locus_tag="OO309_001047"
     CDS             37389..38384
                     /gene="fliG"
                     /locus_tag="OO309_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR5846271.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            38377..39063
                     /gene="fliH"
                     /locus_tag="OO309_001048"
     CDS             38377..39063
                     /gene="fliH"
                     /locus_tag="OO309_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR5846272.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            39063..40436
                     /gene="fliI"
                     /locus_tag="OO309_001049"
     CDS             39063..40436
                     /gene="fliI"
                     /locus_tag="OO309_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR5846273.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            40458..40799
                     /gene="fliJ"
                     /locus_tag="OO309_001050"
                     /pseudo
     CDS             40458..40799
                     /gene="fliJ"
                     /locus_tag="OO309_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            40796..41923
                     /gene="fliK"
                     /locus_tag="OO309_001051"
     CDS             40796..41923
                     /gene="fliK"
                     /locus_tag="OO309_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR5846274.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            42028..42492
                     /gene="fliL"
                     /locus_tag="OO309_001052"
     CDS             42028..42492
                     /gene="fliL"
                     /locus_tag="OO309_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR5846275.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            42497..43501
                     /gene="fliM"
                     /locus_tag="OO309_001053"
     CDS             42497..43501
                     /gene="fliM"
                     /locus_tag="OO309_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR5846276.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            43498..43911
                     /gene="fliN"
                     /locus_tag="OO309_001054"
     CDS             43498..43911
                     /gene="fliN"
                     /locus_tag="OO309_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR5846277.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            43914..44279
                     /gene="fliO"
                     /locus_tag="OO309_001055"
     CDS             43914..44279
                     /gene="fliO"
                     /locus_tag="OO309_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR5846278.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            44279..45044
                     /gene="fliP"
                     /locus_tag="OO309_001056"
                     /pseudo
     CDS             44279..45044
                     /gene="fliP"
                     /locus_tag="OO309_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            45054..45323
                     /gene="fliQ"
                     /locus_tag="OO309_001057"
     CDS             45054..45323
                     /gene="fliQ"
                     /locus_tag="OO309_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR5846279.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            45332..46117
                     /gene="fliR"
                     /locus_tag="OO309_001058"
     CDS             45332..46117
                     /gene="fliR"
                     /locus_tag="OO309_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR5846280.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            46402..47026
                     /gene="rcsA"
                     /locus_tag="OO309_001059"
                     /pseudo
     CDS             46402..47026
                     /gene="rcsA"
                     /locus_tag="OO309_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
     gene            complement(47070..47258)
                     /gene="dsrB"
                     /locus_tag="OO309_001060"
     CDS             complement(47070..47258)
                     /gene="dsrB"
                     /locus_tag="OO309_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR5846281.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            47421..47648
                     /gene="yodD"
                     /locus_tag="OO309_001061"
     CDS             47421..47648
                     /gene="yodD"
                     /locus_tag="OO309_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR5846282.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            47946..48761
                     /locus_tag="OO309_001062"
                     /pseudo
     CDS             47946..48761
                     /locus_tag="OO309_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(48758..50452)
                     /gene="yedQ"
                     /locus_tag="OO309_001063"
                     /pseudo
     CDS             complement(48758..50452)
                     /gene="yedQ"
                     /locus_tag="OO309_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(50623..50805)
                     /locus_tag="OO309_001064"
     CDS             complement(50623..50805)
                     /locus_tag="OO309_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR5846283.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(50884..51801)
                     /locus_tag="OO309_001065"
     CDS             complement(50884..51801)
                     /locus_tag="OO309_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR5846284.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            51974..52894
                     /gene="yedA"
                     /locus_tag="OO309_001066"
     CDS             51974..52894
                     /gene="yedA"
                     /locus_tag="OO309_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR5846285.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(52883..53353)
                     /gene="vsr"
                     /locus_tag="OO309_001067"
     CDS             complement(52883..53353)
                     /gene="vsr"
                     /locus_tag="OO309_001067"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR5846286.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(53334..54752)
                     /gene="dcm"
                     /locus_tag="OO309_001068"
     CDS             complement(53334..54752)
                     /gene="dcm"
                     /locus_tag="OO309_001068"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR5846287.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(54819..55359)
                     /locus_tag="OO309_001069"
                     /pseudo
     CDS             complement(54819..55359)
                     /locus_tag="OO309_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(55399..>55636)
                     /locus_tag="OO309_001070"
     CDS             complement(55399..>55636)
                     /locus_tag="OO309_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846288.1"
                     /translation="WAFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWL
                     GALVGFLFMALVGALLGGIAWYTRPRSARCRRYE"
BASE COUNT        13911 a        14322 c        13461 g        13942 t
ORIGIN      
        1 gattattcac cccacgtttg cgtaattcat gcgcaaaggg caacaggtga taatcgtgga
       61 tccagataat gtcatcgtct tgcaacagcg gcagtaattt atctgccagc aacgcattta
      121 cgcgtagata gccgtcccag gcaggacgct gaaattgcac cagatcgagc cgataatgaa
      181 aagcgggcca gagaacggca ttggagaatt ggttgtagta ttcgtcaagg tcctgttcgc
      241 tgaggttaaa agaggcccac gtaatgttac cttttttagc ggctgatcct cattccctgt
      301 ttcaccactc cagccaaacc acagtccgcc tgcggctttc agtgccccca gtatgccaac
      361 ggcaaggcca ccggcactgg cggcgtgctc gtctggtggt gcaatccggt tagatactac
      421 gactaaacga ctcatagtca tcactcctgt tattttctct tttttgttgt aatgcggcgg
      481 ttatcatttc aagccagctc cagacatccg gcacacccgc cagtcgccat gatgcctgag
      541 ttgcacctgt gccaattttt actgacattc cgcccagtcg gttaacgact gcgaagccag
      601 attcatcggt taaatcatcg cccagaaata cgggcgttcg cccgataaaa ggagcttcct
      661 gcataaaggc tgcaatcgct tcacctttac tggtacctct cggtttgatc tcgacaacac
      721 actttccctg ctgtaacgcc atttgtggcc agatctgagt aatacgttgc gctaatgtca
      781 ttaatgcgtc ttcatgctgc ggagcctgac gataatgcag cgcaaaagcc atccctttcg
      841 cctccagctc cgcgccggga tactgagcga tgaccgtatg cagttgcacg ctaatatcac
      901 gcgcaatcgc atccggcaga tgaacgatat gtgttttacc attgatgtca cggcgctccg
      961 ccccatgcac gcccgctaac gggaagcgat aaggttttgc cagtgcgtca agctccacca
     1021 ttgagcgccc tgatatcaat gccaatgcac catcacttgc ggttgccagt agctgtagtc
     1081 cttgcagaat attgtcaggc acgacgacct gatcgggatg cggtttgatt tccgccagcg
     1141 ttccatcaag atcaaaaaac caggcatatt tcgcggatag ttcaggggtt tcggttaacg
     1201 gttctgtcac ccggctctcc tccttctctt ttcttattgt gttgcgcaga ccgcgcttta
     1261 cagacatgta agtatagaca gtgtgacgga ggtcgccatt tgcaacgaag aacagccaac
     1321 cgttgtggcg gctggctgtg gtgggaaaaa acgttaagtt gttgtggaaa aaagcatcag
     1381 acagtgcgct tcgctttttg cttgtaacgg tcgaagatca ccgctgccag caggattaag
     1441 ccgcgaacca cgtactgcgc gaaaggagaa atattaagca ggttcatggc gttttccacg
     1501 gtgcctaaaa ttaagatacc cgccaccaca tatgagattt ttccgatgcc acctttcaga
     1561 gaaacgccac ctaaaacgca ggcggagata acaataagct cataaccaat cgacgtcatt
     1621 ggctgcccac tagtcatacg tgaagccaga ataattccgg ctatcgcaga taccaggcca
     1681 gagagaacaa agataataat tttggtgcga acaaccggta cacccgccag acgcgcagcc
     1741 tcttcgttcc cgccaatcgc cagggtatta cgaccaaagg tggttttatt cagcagcaaa
     1801 ccaaagataa tcagacacgc gacggtgagc cagattggcg caggcagacc gaaccagtta
     1861 gcgtaaccaa gggcaaagaa gctttcatct tcgataccga ccgctttacc gtctgaaatg
     1921 atgtacgcca gacctcgaac aatctgcatc gttgccagtg tcgtgatcag agcatttatt
     1981 ttcagtttgg cgataacaaa gccattgacc aggccacaga gaacgcccag caacaaaccc
     2041 gctgccacgc caatccacag gctttcagtc aggttgataa ccaccgccgt ggtgacaccc
     2101 gcacaggcaa ttacggaggc gacagaaagg tcaaagtcac cggaagcaag gcagaacaac
     2161 atgccacacg ccaccatccc cgacatggaa attgccaggc ccaacccttt catattaatg
     2221 aaggtggcaa aatttgggac aaaaatggca caggcgataa agagcaccgc aaacaccacc
     2281 agcatgccgt actgatccca gatacgcccg aagctgaatg acgacttagg tgcgccagac
     2341 cccgatgtag aaacagaaga catcatactc tccttactca ggcaactgcc tggctgactt
     2401 taggcatcgc aaggctcagt gcctgacgct catctgcctg ctcgtgtaac aattcaccgg
     2461 cgatttcgcc ttcccgcatc accacaatcc ggtcggcaac gccgaggact tcaggtaagt
     2521 cgctggaggc aaacagcacc gccacgccct gcgccgccag cgcataaatt acgttatata
     2581 tttcgtgctt agcgccaaca tcaatgccgc gcgtaggttc atccagcaaa atgaccttca
     2641 tctcttccga taaccagcgg cccagaatgg ctttttgctg atttccgcct gagagattca
     2701 tgatcagttg ctccgcgccc ggcgttttga tgttgagcga acgaatgtgc tgatcggcat
     2761 tgttttcttc ccaactgttg ttgattacac aaccgccgag cacatgtttg cgtctggcac
     2821 tgatgttgat attgtcgcga acggagtgca cgggaataat gccttccgct ttgcgagctt
     2881 ccgggcagag catcatgcct gcggcaatgg cgtggctcgg tttacgaata tcgatcggct
     2941 gttggtcgat ataaacctga ccggcggtga tttgcgtccc gccaaacaag cctttcatta
     3001 attcgctacg ccccgcccct accagaccaa acagcccaac aatttcacca ctgcgaaccg
     3061 ccagacttat tggcgtacgc acgcctagtg ctttcacagc atcaagacgc aggcgctcct
     3121 cgccataact acgaggttgc cagccgtaga tatcgccaat gtcgcgcccg accatcgcct
     3181 gcaccagcgc gtcgtggtca acctgctgca tatcggtaaa ggttttgaca taacgtccat
     3241 ctttaaagac agtgatggca tcgctgaggg caaatatttc ttccatacgg tgagaaacgt
     3301 ataagatcac ccgcccctct tttcgcagtt cacgaataac gcggaaaaga ttgccgattt
     3361 cgcgggcaga aagggagctg gttggctcat caaaggcgat aattttagcg ttacgcgcca
     3421 gcgctttggc gatttcaacc atctgccact gaccaatggc gagatatttc agcggcgtgt
     3481 ccgggtcaat atccatacca agatgtttaa gttgtaaacc cgcctcataa ttcagcaatg
     3541 agcgattcac aatgccgcct ttatgcggca gctggccgag atagatgttt tccgcgacgg
     3601 tcatttccgg cacgagatgc agttcctggt aaataatcgc cacacccgcg ttaagtgctg
     3661 cggtcgtgtc ggaaaaggac atttcctgcc cattaatcac tacagaaccc gtggttggcg
     3721 catagttgcc gctgaggatt tttaagagag ttgattttcc tgcgccattt tcacccatca
     3781 acgcatgaac ctgaccggca tagcagtcaa aactaatatc cgtcagcgcc ttaacgccgg
     3841 gaaacgtttt accgatgccg cgaaatgaga gatacggggt agactgttgc ataacgtctc
     3901 cgtgaatcac tggtcgttca caccccctct gcatcatgca gaggggaatt tttccggcaa
     3961 attacttacc gcctaaacct tttttctcca gttcttcttt aaagttgtca cgcgtgatca
     4021 gtaccacgtc tgtaacttcg gtaaattttg gcgtttcaac gtcttttgct acccagttgt
     4081 aaagcatttc gctggattta tagccatgta cgtccgggct tggcagcagg gaaccgtaga
     4141 agccggttgc ctgtgcttta gacagttcgc ttactgcatc cacaccgtta ataccaatac
     4201 cgatgatatc ggctgcttta aagccctgac cttcggttgc acgtacgccg ccaagtacgg
     4261 tgctgtcgtt cataccgacg atcagccaat gtttaacttc cggatgttga accagcattg
     4321 agttggcagc gtcaaatgcc cccgggatgt cgttagattt ggtaggtacc tgataaattt
     4381 gtttttccgg gaatcctgca gctttcagtg cgtccataga acccgttgta cggcggcggg
     4441 cggtatccag ttcgttggcg gtaatcgcca tcactgcgct ttctttgaca tcccagccac
     4501 gtttctgcat ctctttatac agttcctagc cctgacgttc gccaatttta gtcgccgcca
     4561 tcatcaccag aggaacggta tccattggct tacctttggc gttaacaaac tggtcatcca
     4621 ctgcaatgac tttcatatcg tagccacgcg ctttcgcgac gatggcagag ccgagtttcg
     4681 ggtccggagt acaaataacg aaaccttttg cgccactggc tgccaggctg tcgatcgcgt
     4741 tcaatgtttt ttcgccatcc ggcacggcaa tcttaataac ctcaaaccct aaatccttcc
     4801 cggctttatc ggcaaacttc cattcggtct ggaaccacgg ctcttccggt tgcttcacca
     4861 gaaaaccgag cttcaggttc tccgccatag cggattgtga cataacggct gccagaccaa
     4921 tggctgccag ggctttagta aatttgtgca tggttctctc cagctttagt gtcgttttgt
     4981 gtagggcaaa aacgaatgac attcgttaaa ttaatcggaa aacaaagcat taccttttat
     5041 ctaaaagata agtgaccgtg ttgacatagt tttagcgaga aattaattct ccataggaga
     5101 gcaatatcac atcgcagaat tacagtgaga acgtgcataa atttagcggg aaaagacata
     5161 aggaaaagcc aatttgtcag acaaattgtt gaatgcacag cagattaatc cataagatta
     5221 gcctggaaat ccttgttgtc ttcggtaccc atgcgggatg tcttcttttt aaccagtcaa
     5281 taggccgcat tacctggcgt tgagtttttg aaatggtgta ataaccacaa ctcaaagatg
     5341 tggaaaatgc gcgtcattca tttcgccatt aattatcact gtgctcatta attaacagaa
     5401 cacgtataat gagagccatc tcgcaaaaat gaaaaaacgt tttataaaat catcacttca
     5461 tcatgaattc aaattcatcg attaatatca acaagataca aaaagcacta tcattaaaat
     5521 tcattgcatt tacattgatt tcaccaatga aatgtaaaaa tatataaact tgatgattta
     5581 agcattttct tataaccgtt cagacgttat tcttatttca gatcatcgtc agaattgact
     5641 ccacgatcac atttcggacc ggcagaaagg aattattctg caaacagtaa ttatggtgtt
     5701 ttgatttatc ttgcacctct ccacttctgg atataaggat attaggtatg gcaaccgctg
     5761 gaatgcttct caaactcaac tctcaaatga accgcgagtt ttacgcatcc aatctctacc
     5821 ttcacctgag taactggtgt tctgaacaga gtctgaacgg caccgccact ttccttcgcg
     5881 cccaggcaca gagtaatgtg acccaaatga tgcgcatgtt taactttatg aagagtgtcg
     5941 gcgctacccc catcgttaaa gccattgatg ttccaggtga aaaactgaac tctctggaag
     6001 aactgttcca aaaaacgatg gaagaatacg agcaacgttc aagcactctg gcgcagttag
     6061 ccgatgaagc gaaagaactg aatgatgatt caaccgtcaa tttcctgcgc gatctggaaa
     6121 aagaacagca gcatgatggc ctgttgctgc aaaccattct tgatgaagtg cgcagtgcga
     6181 aacttgcggg tatgtgccct gtgcagaccg accaacatgt tctgaatgtc gtgtcacacc
     6241 agctgcattg atcatacatc ggcgctaata cattgcgccg atgaagtttt tgagaaaccg
     6301 ctgcctcatc tgtttgaagc agcggttttt ttaatgggat tcaccctgtg gggtaaactt
     6361 gagttcaatc agcgcgatgg ctttttggat tgcccgcatg gtgaccgggt ctgcggcagc
     6421 gggatgatga gtaaagtcga tattcttcag ctgactggac attttttcac gaacttcaac
     6481 gggcgcgatt acatcgagaa catccagaat ttgtttgata accaactggc aagctaccac
     6541 atcagaaacc agttcctgat cggcattcag cggctgggac atcgtaaact cctgatagca
     6601 ttttgaaagc cgttatagta gcggcttcac atcttcagcg atagtcacat ccaccgtcat
     6661 caggacacaa aaaaacctgc cggagcaggt tttttgttat cggaacatat tgcctggcgg
     6721 tacgtctttg aacgtcttgc aatagttatt gaacatactt ttcaggattt tgcgcagttt
     6781 catcgcggca ctccgaccat ttgttataca ggtgttattg tctttgcgct tataatatga
     6841 caaccatcac aaaaatcaat ctttatgtga tacaaatcac ataaataccc ctttaatgtt
     6901 ataaaaatga taatcaaaaa acagcccccc tatttctgac acctacagat ggcaagaaat
     6961 agcgcctgcc aggcgtcttt tccggccatt gtcgcagcgc tgtaacgcgt aaaatagtgc
     7021 tttctcttac tcttctggct ggaccatgag acttctgatt ctgactcttt cattaataac
     7081 gctggcgggg tgtacggtta ctcgccaggc ccatgtgagc gaagttgacg cggcaacagg
     7141 tattgtacgg ttggtttatg accaggcttt tttgcagcat gcccataccg atcgttatgt
     7201 cagtcgcggg atcgccgatc gcgcatgcca gcaggaaggc tatacccacg cgatcccctt
     7261 tggtcagcca gtaggcaact gtagcctttt tgcgggttcc ttatgtttga atacggaatt
     7321 cactttgtca tatcagtgcc atcattccgc cttccctgtc tttctctaaa tattttaatc
     7381 agcgagggga tcttcgctga ttaaagaaat agcggtaatg cgttttattc ccattcgtat
     7441 ttttaataat tgaagtttat attttacctt ttgcaaataa taaaataaca aattatagtg
     7501 acgccacagc aacaaatata acctttgtgg agcactatca tgctgaaacc agaaatgatt
     7561 gaaaaactta atgagcagat gaacctggaa ctgtactctt cactgcttta tcagcaaatg
     7621 agcgcctggt gcagctatca taccttcgaa ggtgctgccg cgttcctgcg ccgtcacgcc
     7681 caggaagaga tgacgcatat gcagcgtctg tttgattacc tgactgatac cggcaattta
     7741 ccgcgtatta ataccgttga atctccgttt gctgaatatt cctcacttga tgaattattc
     7801 caggaaacct ataaacacga acaattaatc acccagaaaa ttaacgaact ggctcatgct
     7861 gcaatgacca atcaggacta cccaacattt aatttcctgc agtggtatgt ttctgagcag
     7921 catgaagaag agaaactgtt caaatcgatt attgataaat taagcctggc aggcaaaagc
     7981 ggcgaaggtc tgtattttat cgacaaagaa ctctctaccc tcgacacaca aaactaatgc
     8041 tctccggcgg caggtttctg ccgccgtttc aaatcagtga cggcaaatct tactctgatc
     8101 ggtcgaaaaa ccttcttctg aaggtataaa tttgcctttt gctgccagaa acgctaccag
     8161 ctctcccgct gtcacccctt ctgccgagca cgtgtgaaaa cgtgcctgtt caccaaaacg
     8221 cgctttaatc gcagcttcca gactggcatg cgtatattgc tcgcctgatt caatcatcat
     8281 atttaacact tcatgaccgt gaatagagtc catcgttcct cctcaaaaaa agcctagcgt
     8341 agcgattacc gcttatgaag actttgcgcc agcgcaggac tgaatgcttt tattgtacat
     8401 ttatatttac acaatatgta acgtcggttt gacgaagcag ccgttatgcc ttaacctgcg
     8461 ccgcagatat cactcataaa gatcgtcagg acagaagaaa gcgtgaaaaa cagaaccctg
     8521 ggaagtgttt ttatcgtggc gggaaccaca attggcgcag gcatgctggc aatgccgctg
     8581 gctgcggccg gtgttggttt tagcgttacg ttaatcttgt tgattgggct ttgggcgttg
     8641 atgtgctaca cggcgctatt actgctggag gtgtaccagc atgttccggc agataccggt
     8701 ctgggcacgc tggcaaaacg ctatctggga cgctacggtc aatggctgac gggcttcagt
     8761 atgatgttct taatgtatgc tctgactgca gcatacatca gcggtgccgg tgaattgttg
     8821 gcctccagca tcagcgactg gacaggtatt tcaatgtcgg caaccgctgg cgtgctgttg
     8881 ttcacttttg ttgccggtgg cgtggtttgt gttggaacat cgctggtcga tttatttaac
     8941 cgttttctgt tcagcgccaa aattattttt ctggtggtga tgctggtatt gctgctaccg
     9001 catattcaca aagtgaatct tttaaccctg ccgttgcaac aggggctggc actgtctgca
     9061 atcccggtga tttttacgtc gtttggtttt cacggtagcg tgccgagtat tgtcagctat
     9121 atggatggca acattcgtaa gctacgctgg gtgtttataa tcggtagtgc catccccctg
     9181 gtggcatata ttttctggca ggtggcgacg cttggcagca ttgattcaac aacctttatg
     9241 ggattgctgg ctaatcatgc tggattaaac gggctgttac aggcgttacg cgaaatggtg
     9301 gcctctccgc atgttgagct ggcggtgcat ttatttgctg atttagccct cgccacgtca
     9361 tttctcggcg ttgcgttagg cttatttgat tatctggctg atttgtttca gcgttcaaat
     9421 accgttggtg gacgattgca aactggtgca attacctttc tgccgccgtt ggcgtttgca
     9481 ctgttttatc cacgaggatt tgtgatggcg ctgggttacg ccggtgtggc gctggcggta
     9541 ctggcattga ttatcccttc actattgacc tggcaaagca gaaagcacaa tcctcaggcg
     9601 ggttaccggg tcaaaggtgg tcgtccggcg ctggtggtgg tgtttctctg tggtattgct
     9661 gtgattggcg tgcaattttt gattgcggca gggttgttac cagaagtggg gtgatcagat
     9721 agcctcaaat tccttattgg gtgccagaat taacgctgac acccaatgtg acctcttaat
     9781 gcaggcagca ctgcttaaat ttcttaccac tgccgcacgg acaaggatcg ttacgccccg
     9841 gtttctcttc tgctttgatc ggttgctgaa cagctttttc ctgcggatgc gccatccagt
     9901 atgcatgtaa atcaagcgcc gccagtcgaa tggcatctac gctctcttca aacgcttctg
     9961 gcgacatttt ctctacccgc tcgaagtttt cctcagtacc gtgcagcgca atcgcctcca
    10021 gcgctggttt taaagaatcg ggcaacgttg accagtcaga aagtgccacg ccccgcatat
    10081 agccaaagca ccactcctca acaatcgtca gctcgctgcc atcaacttct cgcaagccga
    10141 ataacggctc aaactgctcc gggaattcgt tcagacgctc tgcggtatcg gccatatgtt
    10201 gaaaagccag gttcataaag cgcgtcatct ctttctctga cgcccaacgc ggcacatagt
    10261 cagccccacc ccacacggca accagccact gttctggttc aatctcttgc ggagaactca
    10321 acaccgccgt caataaaccg tccagctccg ccacatcaag gatggcgtgg tcagtgttgt
    10381 atttggtcag aatatcgtcc agccattcca actcactttc gtttaacggt cccgttttca
    10441 tacgcttttc cttgtggatc tcaactcgcc agcacctatc ttacatgccg gtccgtatca
    10501 gagatacttt ttgagtggct ttgccggtga ttaaaaatta aggagggtgt aacgacaagt
    10561 tgcaggcaca aaaaaaccac ccgaaggtgg tttcacgaca ctgcttatta ctttgatttt
    10621 attcttatct ttcccatggt acccggagcg ggacttgaac ccgcacagcg cgaacgccga
    10681 gggattttaa atcccttgtg tctaccgatt ccaccatccg ggctcgggaa gaaagtggag
    10741 gcgcgttccg gagtcgaacc ggactagacg gatttgcaat ccgctacata accgctttgt
    10801 taacgcgcca aattcttcag gcctttcagc cagacatccg cttgacgccg atgtctttta
    10861 aactggagcg ggaaacgaga ctcgaactcg agacctcgac cttggcaagg tcgtgctcta
    10921 ccaactgagc tattcccgca ttcatcaagc aatcagttaa tcacttgatt ttattatcgt
    10981 ctggcaatca gtgccgccgt tcgatgcgtt gcattctact tacctggcgc gatgagtcaa
    11041 cgatattttt caccactttt gatcgtttgc tgaaaattac gccgaaacga tcactgatca
    11101 agcaaatctg cacgcgcagc gctcaaatat tgcaacattg accacagagt cagtaccgca
    11161 gccacaaaga aaagtgcaat accgacgtac tcaacccaaa tgttcggacg ccacagcagc
    11221 caggccaacg ccaccatctg ggcagtggtt ttcactttcc caatccagga gacggccacg
    11281 ctactgcgtt tacccaactc cgccatccat tcgcgtagcg cagaaataat aatttcacgg
    11341 gcgatcatcg ttgccgccgg taatgtcacc caccagctgt gataatgctc ggttaccagc
    11401 accatggcga tagccacgag aactttatct gccacagggt caaggaaagc accaaaccgg
    11461 gtactctggt tccagcggcg tgccagaaaa ccatcgaacc agtcagtcac cgccgcgacg
    11521 cagaaaatga gcgcggcggc aaacggcgac caggtgacag gcagataaaa gaccaataca
    11581 aagaatggga taaggatgac acggaacagt gtaagcaacg tagggatatt aaattgcata
    11641 atgacgggta actatctgtt gtcagtaaga ttacccctat gttgctacag agacatcaat
    11701 gtttcaacga ccagaagatc ttttctgcca gaccttgcga aatacccggc acttttgcaa
    11761 tttcctcgac gctggcgtta cgtaaacctt gcaaaccgcc catatatttc aacaacattt
    11821 gccgacgttt tggcccgacg ccttcaatgg tttccaggga actggtattt ttgaccttcg
    11881 cccgtttttt acggtgcccg ccaatcgcgt gatcatgtga ttcatcgcga atatgctgga
    11941 taacatgcag cgcgggtgaa tctggcggca aactaaatcc ctcaccttcc ggctcaaaga
    12001 acagcgtttc cagtccagcc ttacgatctg ctcctttggc aacgccaagt agcagcggat
    12061 gatttttatc ccatgagaca tccagttcgg cgaagacatt tttcgcctgc gcaagctggc
    12121 ctttgccgcc gtcgataagg atcacatccg ggatcttact gtcgtcaatg gctttaccat
    12181 aacgccgacg cagcacctga ttcatcgccg cataatcatc gcccggcgtg atgcctgtaa
    12241 tgttatagcg ccgatactcc gcacgcagcg ggccgttagc atcaaacacc acacaggaag
    12301 cgacggtttg ttcgcccatg gtatggctga tgtcaaagca ctccatccgc ttcacttccg
    12361 gcaatttcaa cacgctggca agcgcggtca gtcgctggtg aacggtagat tgctgcgaaa
    12421 gtttgctggt taaggccgtc gccgcattgg tgcgcgcgag tttcagataa cgcgccctat
    12481 cgccgcgagg tttggtttga acattaatct tgcgtcccgc cagttctgaa agggaatcgg
    12541 cgagcagcgt tttatcgcta agattaaaat cgagcaggat ctcacccggt aaggtgcgca
    12601 tctggctgcc ttgtaaatag aactgaccta cgaaggtttc taccacctcg ctcagttccg
    12661 taccgccagg cactttcggg aaatagctgc ggctgccgag cactttgccc tgacgaatga
    12721 acaatacgtg gacacaagcc atgcccgcat cgaacgccac accaataacg tcaaggtcgt
    12781 cgccggtatt ggaaacaaac tgtttttcgg tgacgcgtcg caccgcctga atttggtcgc
    12841 gaatacatgc cgcttcttca aactccagat tctggctggc agtttccata cggctaatga
    12901 gttgcgtaag cacctgatca tctttgccag acaaaaacag gcgcacatac tcgacctgct
    12961 gagcgtattc ttcttcactc accagtcctt caacgcacgg tcccagacag cgccctatct
    13021 ggtattgcag acacggacgc gagcgattgc gataaacact attttcgcac tggcgaatgg
    13081 ggaaaatctt ttgcagtagc gccagtgttt cacgtacggc atagccattc gggaacgggc
    13141 cgaaatattc acctttggca tgctttgcac cacgatgcat cgccagacgc gggtgggtat
    13201 cgccactcag gaagataaaa ggataagatt tatcatcgcg tagcaaaacg ttgtaacgcg
    13261 gctgatagag tttgatgtag ttgtgttcca gcaacagcgc ttcggtttct gtatgcgtaa
    13321 ccgttacatc aatttgctgg atctgggcga ccagcgcttc ggttttgcgc gaagcgaggt
    13381 tgctgcggaa atagctggaa agccgttttt tcaggtcttt cgctttgccg acatagataa
    13441 ccgtaccacc agcatcgtac atgcgataaa cgcctggctg gctggttacg gtttttaaaa
    13501 acgcttttgc gtcaaactga tcactcactg acttgataat gtctccgcat tacacagacc
    13561 atggcgaatt gccaggtgag tcagctcaac atcgccatga atgtttagtt tactgaacat
    13621 acgatagcgg tagctgttca ccgttttcgg actgagattg agctgttctg agatctcatt
    13681 gaccttctgg cccttagtga tcatcagcat aatctgcaat tcacgttcag acaaactggc
    13741 aaatgggctt tctgtttttt ctggttcgat ctggcttaac gccatttgtt gagcgatatc
    13801 agaagcaatg taacgctgcc ctgaatagac agaacgaatc gcactcacga cttcctgcgg
    13861 agccgcgcct ttgctgaggt agccagcagc acctgcctgc atgactttcg ctggtaaagg
    13921 gttttctgta tggaccgtaa gcatgatgat tttaacatca gctgtggaac gcgcgatttt
    13981 acgcgtcgcc tcaagaccgc caatgcccgg catactcatg tccattagca ccacgtcaac
    14041 ggcatttgtc cggcaccact taacggcgtc ttcaccgcac gatgcctctc cgacgacttt
    14101 tataccctta atatcttcca gaatgcgtcg tatccctgcg cgcaccagtt cgtggtcatc
    14161 aacaagtaga acgttgatca aaggaatatc tccagaaata gggataacgc tactgatagt
    14221 tagtcattcg tatattagcg gtttttattg caactttgaa acgttaaaaa tggtgggtat
    14281 tcgatttttc tctcgttttt gtcaattcga ttgtccacag ttatggtcac gcccgtcacc
    14341 aacggcttac cggatcgttt cacagtaagc cctacgatga aatgtgacaa aaattgactt
    14401 tattcagcaa aaatgaaaat cagcccgctg aatatgtaac attaattaac ccgaacaata
    14461 cgggcaaaaa acattcatga taaaaatatt tattggtcat tatattaacg tattttatag
    14521 cactgccgat atcacgctca aaaaacaacc actgctattt ttagcaaagc ttatggtata
    14581 ctccgccgcc ttaacatttt tcaccacaaa ttttcattgc aacatgacga ggaaaataaa
    14641 tgagtacgcc tgatttttct actgccgaga ataatcaaga actggcaaat gaagtctcct
    14701 gcctgaaagc gatgctgacg ctgatgctgc aggcgatggg acaagctgac gcgggccgcg
    14761 tgatgttaaa gatggaaaaa cagcttgcgc tgatcgaaga cgaaacccag gctgcagtat
    14821 tttccaaaac ggttaagcaa attaaacaag cctaccgtca gtaatataaa accggctgag
    14881 agcgtgcctt tcagccggtt attgacaaaa tcatcgctat ctctgctgat gtcttccctt
    14941 ccgccgtcca tttcagaatt tctttttcgc gcttgctgaa attcatctct ggcgtcatca
    15001 ctatttcatc atttaaacgc atcagagcca tcagactttc gcgcaccagt aactgcattt
    15061 ttaattgcag ttcatcacta agaatgggta tttcgcgcgt gctgcaacgg gaaaaggaca
    15121 aaaagcccag cgcccgattt ggcagcatta aatactgagt gacaccgcgg cgtaaaccat
    15181 gtgcgtgcgc ggcttcccat aatggctgtg cttcgctgaa taagtcatca ttccacatta
    15241 aatggccctg actaaagttt tcagggttca gcacaggatc aattgcgaga aaattttttg
    15301 cctgataata actaacccac gactcagggt aattggtgta gaaagccact ttaggtcgcg
    15361 tgaatggtac cgggtggcga acacataacg aatagtaatc gtactccagc tgctgagcct
    15421 gaagctctat ttcatggtag gcctcttctg cggcctccat cctctgaaaa cgcaacagca
    15481 tcgtgcgacg ccagctgaaa aaatccgtat cctgcatagt aaaccgcaac gcccctgaga
    15541 gtgagtatca tttataatga taattcaaac tatcacataa ataagattta tatataattt
    15601 atattattca ggcaatgaat tacttttgca agccatcgca ttctcttatg ttattaatga
    15661 gttatgctga tttgttaagc agttttatca cgcttgaaat ggcgtcccgc cccgacaggt
    15721 gaatcgtcgg ggctgatttt ttcttattat tgcagcagaa gcttctcgag gaactggcgg
    15781 gtgcgaggct gctggggatc ggcaaataac gcttttgctg ccccctgctc gactatccgc
    15841 ccctggtcca taaagatcgc ccggtccgca acatcccggg caaagctcat ttcgtgtgtc
    15901 acaatcacca tcgtgcgctt ttcctgcgcc agctggcgga tggtgttcag gacttcgccc
    15961 accagctctg gatccagcgc cgacgttggc tcgtcaaaca aaatcacctc aggacgcatt
    16021 gccagtgcac gcgcaatcgc aacacgctgc tgttgaccgc cagacaaacg acgtggatag
    16081 ctggtttctt taccggccag cccaactttc gccagcagct cgcgagcgcg cgccgtggcc
    16141 tcttctttcg gttcgccttt gacgatcacc ggcccttcaa taatgttttc cagcaccgta
    16201 cgatgcggaa acaaattaaa gttctggaag acaaacccga cgtgctgacg caactggcga
    16261 atcagcgatt tttgctgact taatgaacgt gcagtatcaa tagtgatatc gccaacggtg
    16321 atcgttcccg cttccggttg ttccagcaga tttatgctac gtagcaatgt ggttttgccg
    16381 gaaccactcg gaccgataat tgccaccact tcgccaggct ttacctcgag gtcgataccg
    16441 tgcagcaccg tctgaccgtg gaattttttc accaggttct taacttcaat ggcactcatt
    16501 ttggttctct ctcctggcga ttaagttgat tctcaaaatg gttctgcaac gtcgataaca
    16561 cagtcgccat gatccagtag atgagcgaag ccgccaggta catggtgaac acctccagcg
    16621 tgcgcgaggt aatcaactgc gcctgacgga acagctccgg cacctggatt gtcgcggcca
    16681 gcgacgtatc ttttaccaga ctgataaagc tgttcgacag cggcggcaac gccacccgcg
    16741 ccgcctgtgg caaaatcgcg cgacgcatgg tctgccacgg cgtcatacca atactggcag
    16801 cggcttccca ctgaccttta tcaatagaag aaatggcggc gcgcagcgtt tcggcggcat
    16861 aggcggcagt gttcagcgac agaccaatca ttgctgacgg aatgggatct aattcgatac
    16921 caaactgcgg caggccgtaa tagatcataa acagttgtgc gatgagcggc gtaccacgaa
    16981 aaatagagat ataaaaacgc gccagccagc gcacgggcca gattggcgac aggcgcatca
    17041 gcgcgagaat aaacccgagt agtaagccaa aaaacatgcc gccgatactg agttgcaggg
    17101 tatacccagc ccctttcaac aggaacggca aggaatcaat aaccagttgt atactttctt
    17161 gcatgagcgt tcccggagct aaacgtgggg atgataggcg aacagcgcag gcgcgccgcc
    17221 ggtatgaata aacagaatcg gcccttcatc tttgaagcgt ttctgactga taccgtcaat
    17281 cagccccgcc atcgcttttc cggtatacac aggatcaagc agaatgcctt ccagacgcgc
    17341 cagcaatttc actgcttcca tgccttcgtc gttcggcacg ccgtagccag gtgcaaaata
    17401 gtcatcccag agtaaaattt ccgctgatgc ggtcagctcc agttctttcg caatcgcctg
    17461 ttgtaggtta accactttcg gcaattgatc ggcaacggaa cgcgacacgg tcacgccaat
    17521 cagttcgctt tcaggcatca ggtgttccag cccaacagcc agtccggcgt gagttccggc
    17581 actgcccgat gcgactacca ccgacgaaat attaaccgcc ccttcacact gttgcgcgat
    17641 ttccagcgca ctctccacat aacctagcgc gccaagagca ttagaaccgc caaccggaat
    17701 gacatacggg cgaaagcctt gtgcttcgac tcgcgtcgcc agttcttcca gttgggcatt
    17761 gggatcggtc agtgcgtcgc acatttcaat ctgggtattg aacagatcca gcaacaaacg
    17821 attgccgttg gttaaatagt tttctgcggt tgtgccaata ggattttcca gcagcgccac
    17881 gcagtgcaga ccgagtttcg ccgcgactgc ggcagtctgg cgcacatggt tagactggat
    17941 cgcgccggca gtaatcagcg tatcggcacc ttcgcgcagg gcatctgccg cgagaaattc
    18001 cagcttacgt aatttattgc cgcccattgc catgggggtg acctcatccc gtttgatgaa
    18061 aatttcccgt ccaagataat cagaaaagcg cggcagatat tcgagcggcg ttggcgcgcc
    18121 gataaactcc aaccgtggaa aacgggttaa attatgcagt ggcataacag cctccgatgt
    18181 gtgttgttgt gattttctta ttatgcacgc tgaaaacgcg taaataaaaa aggcgctagt
    18241 gaaagcgccc ttttttgtca ttatgctgat tatttggtca catcagcacc aaaccatttt
    18301 tcggaaagag cttgcagagt gccgtctttt tgcatttccg caattgcatc attcaccgct
    18361 ttcagcaggt cttcatttcc tttacgcagc gccacgccag actcctgacg ggagaatgct
    18421 tcaccggtta ctgccagcgt atcgttggtt ttcttcacca gatccagcgc cgccagacga
    18481 tcaacgagga tcgcatcgat acgccctacg cgcagatcct gatatttggt cgggtcatca
    18541 tcataggtac gcacatcgac accctgaaca ttctgccgca gccactcttc atagttggtg
    18601 cccagaccga cacccacttt tttgcctttc agatcagcag ccgttttaat ggtgccttcg
    18661 ttaccttttt tcaccagcgc ctgaataccg gaaatggtgt aaggggtaga gaaatcgtat
    18721 tttttcttgc gctcgtcaga aatggtgacc tgattaatca ccacatcaat gcgtttagag
    18781 tccagcgacg ccagcatacc gtcccatttg gtcggtttta gtgacgcctc aacgccaaga
    18841 tgttttgcca gctgttgggc aaattccact tcaaaaccgg ttaatttgcc gtcatccccc
    18901 tgaaaactga acggcggata agtgccttcc agccctacca gcagcgtgcc gctctctttg
    18961 actttattaa gcagaccttc atctgcaaaa cttttaacgc tcatgcccgc aaccagcgcc
    19021 acggccatca cacccatcaa tgcctgacgt cccagatgtg ctaatttcat attcaccccg
    19081 aatgttgtta tgtctgtttg cagtgtagag ccattgtttg taaacacaaa aacaactccg
    19141 ctacatctta ttcttattta atatatatca gaagcaggca ggctggagga tttctgcaca
    19201 agtccggtac aggtttggcg ctgatagtgt tgatacttcc gtaatgcgat gcggtaattg
    19261 ttggtgctgg tggtgggcag ccacggggcg aggatttcat caaggaaacc gtcctgcagc
    19321 tgatcgaggg aaatattttg ctcatgtaga tgattaccca aacggcgcag acgaacgaca
    19381 tattcacgca ccgtacctgg gctcatttca gtttgctcaa aaagaaattt cttaaagccg
    19441 ataatatcga aaaaatcact ctgcgttttg caatgtaaat caccgcaaaa tcggcacaat
    19501 gccgcccatg attgttgttc cgtttgccag cctttttcat caagcagcgt gtccaggcgg
    19561 gaaatctcga ttttattcac tattttgccg tcgcgaacta aggtaatgcg atcgagtaat
    19621 ttacggcaat gcgcgcaatg ggtctggctg tgtttaaagt ctttaagata gcggcttaat
    19681 ggccgtcttt tcaggtgctg caccatcatt aagaactcct ggtagtcaaa gttaaagtgc
    19741 ggcatttact gacgttataa cttacccagt ttagtgcgta accgtttaat gtcctggctg
    19801 tgtaactgac tgacccgcga ttccccgacc tccagcaccg cgccaatctc tttgagattc
    19861 agctcttcct ggtaatagag ggttaatacc agtttttcgc gctccggcaa cgtttcgatg
    19921 gcttccatca cccgctggcg cagattactg tccagcagtt gttgtagcgg gttttctcgc
    19981 tgatgatcat cagtaaccag ttcgatgcta tcgccgtgct cttcacgcca ctcatcgtag
    20041 gagaagagct ggctgttatt ggtgtcgagc agcatttgac gataatcggc aatatcgatc
    20101 cctaaacgtt ccgctacctc agtttccgtg gcgttgcggc caagttcctg ctccagttgc
    20161 cctattgcct gtgccacttc acgcgcgttg cgtcgcacgc tgcgcggcac ccagtcacgg
    20221 ctgcgaagtt catccagcat agcgccacgg atacgctaca ctgcgtaagt tgtaaatgcc
    20281 gttccttgta gggcgtcata gcgttcgaca gcattaagta acccaatgcc gcccgcctgt
    20341 agcagatcgt caagttccac gctcgcgggc agtcgaacct gcaggcgcaa tgcttcgtga
    20401 cgcaccagcg ggacataacg ctgccacagc gagtgtttat ccattacacc ttcagcggta
    20461 tagagtgaat tcacgataaa cagccctgcg ttatatgagt tatcggcatg attatccgtt
    20521 tctgcagggt ttttaatcgg acgattagtg ggtgaaatga ggggttattt gggggttacg
    20581 caaaaagccg aatgtaaaaa accccgccgg tggcggggtt tgagaggcat tcagacgatt
    20641 aaccctgcag cagagacaga acctgctgcg gtacctggtt ggcttttgcc agcacggagt
    20701 taccggcctg ctggataatc tgcgctttcg acatgttgga cacttcggtc gcatagtcgg
    20761 cgtcctgaat acgggactgc gcttcagaca ggttggtagt ggtgttgttc aggttggtga
    20821 ctgcggaatc caaacggttt tgcaccgcac ccagggagga gcggaatttg tcgatggagc
    20881 tgatggcttc atccagagct ttcaggggat cggctgtcgt agctgctttt gtttcagcat
    20941 ctgtcgtcaa tttaccatca gcatctttat aaactgcctt accgctgccg ttagtcactg
    21001 aaccatcatc ctgtaggtaa aaatctacag tatcagatgt ggtcaacgcc tcgttgttat
    21061 caacataaac cgaatcaaca ccttttgtta cagcgccagt tgtgccgttg acgctataac
    21121 ctgcagtagt gaaatcaaca ttgtttcccg aggttgatac agctttatcg aaagctgcct
    21181 gatcaacttt tgcacctgtt actgaataag taatagtgtc cggcgtagta ctattaccgg
    21241 taaaggagat agaggtaccg ccaatgtcaa tggttgtcgc tttggtatta ttggctgcaa
    21301 gtgtggacag actagcctca gtcaaagaag caccagagcc gtttttcgtt aagcgcccag
    21361 ttgtatcaat gtagagttta tctccattgc ttgccgtaat tttaccatca ctgctgatct
    21421 gaacatcctg tgcagaacca tcgatagtaa tagtgccctt agcggtatca ccaactcccg
    21481 gggtcaaata tttctgaaca tcggcagctg aagccgttgt ggtatcaaaa gagtatgact
    21541 tacttgcact gtcatacttg taattcgttg cactagccgc cgttccgaag ccgttattaa
    21601 tggttgcctg aacagtatca ccatcactaa ctccagccag caaatcagac gctttagcag
    21661 catcgtaacc cgcactcaca gtatatttgt tgccgaccac tgttgcatta gcagctacca
    21721 agtcagcttt agatgcagca gtattagcca cagccccgcc accattcaca ttaaacccat
    21781 tcagccccag cgtatcagag tcaattttct tcagatcaat agtgatagtc tggccgtcat
    21841 tcgcaccaac ctgaattttc atcgaaccgt cttttgccag tacgttcacg ccgttgaact
    21901 gggtctgacc ggatacgcgg tcaatttcgt cgagacggga tttgatttcg tcctgaatgg
    21961 agtccagatc cgaatcagag ttagtcccgg tagaagcctg aaccgtcagc tcacggatac
    22021 gctgtaagtt gttgttgatt tcggacagcg cgccttcagt ggtctgtgca acagaaatac
    22081 cgtcgttggc gttacgtgca gcctgagtca ggcctttaat gttagaagta aaacggttag
    22141 caatcgcctg acccgcggcg tcatccttcg cgctgttaat acgcaagcca gaagacagac
    22201 gctcgataga actcgacagc gcagactggt tcttgttgat attattttga gtgatcagcg
    22261 agaggctgtt ggtattaatg acttgtgcca tgattcgtta tcctatattg caagtcgttg
    22321 attacgtttt gggtttccac ccgtcggctc aatcgccgtc aaccctgtta tcgtctgtcg
    22381 taaaacaacc tttagaattt ttttcacaaa cagccatttt ttgttagtcg acgaaatact
    22441 cttttctctg ccccttattc ccgctattaa aaaaaacaat taaacgtaaa ctttgcgcaa
    22501 ttcaggccga taaccccggt attcgtttta cgtgtcgaaa gataaaagga aatcgcatgg
    22561 caagtatttc atcgctggga gtcgggtcag gtctggattt aagttcaatc cttgatagcc
    22621 tcaccgccgc gcaaaaagcg acgctaaccc ctatttcaaa tcagcaatcg tcgtttaccg
    22681 ctaaacttag cgcctacggt acgctgaaaa gcgcgctgac gactttccag accgccaata
    22741 ctgcattgtc taaagccgat cttttttccg ccaccagcac caccagcagc accaccgcgt
    22801 tcagtgccac caccgcgggt aacgccatcg ccgggaaata caccatcagc gtcacccatc
    22861 tggcgcaggc gcaaaccctg accacgcgca ccaccagaga tgatacgaaa acggcgatcg
    22921 ccaccagcga cagcaaactc accattcaac aaggcgacga caaagatccg attaccattg
    22981 atatcagcgc ggctaactca tcgttaagcg ggatccgtga tgccatcaac aacgcaaaag
    23041 cgggcgtaag tgcgagcatc attaacgtgg gtaacggtga atatcgtctg tcagtcacat
    23101 caaatgacac cggccttgat aatgcgatga cactctcggt cagcggtgat gatgcgctac
    23161 aaagttttat gggctatgac gccagtgcca gcagcaacgg tatggaggtc tcggtcgccg
    23221 cccagaatgc gcagctgacg gtcaacaaca tcgccatcga gaacagcagc aacactatca
    23281 gcgacgcgct ggaaaacatc actctgaacc tgaacgatgt caccacgggc aaccagacgc
    23341 taaccatcac tcaggacacc tccaaagtgc aaacggcgat taaagactgg gtgaatgcct
    23401 ataactcgct aatagatacc ttcagcagcc tgaccaaata caccgccgta gatgcgggag
    23461 ctgatagcca gagttctagc aatggcgcac tgctcggcga ctccacgctg cggacgattc
    23521 agacgcagtt gaagtcgatg ctgagtaata ccgtcagttc ttccagctat aaaacgctgg
    23581 cgcagattgg tatcacgacc gatcccagcg atggcaaact ggaactggat gccgacaaac
    23641 tcaccgctgc actgaaaaaa gatgccagcg gcgtaggtgc attgattgtt ggcgatggta
    23701 aaaaaaccgg catcacaacc accatcggca gcaacctgac cagttggctt tcgacaacgg
    23761 gcattattaa agccgctacc gatggcgtta gtaaaaccct gaataagtta actaaagact
    23821 acaacgccgc cagcgatcgc attgatgcgc aggtcgcgcg ctacaaagaa caatttaccc
    23881 aactggacgt tttaatgacc tcgttaaaca gcaccagcag ctacttaacg cagcagttcg
    23941 aaaacaacag taattccaag taatcattat tcatcgggag acaggtcatg tacgcggcaa
    24001 aaggcaccca ggcctatgca caaattggcg tcgaaagcac cgtaatgagc gccagccaac
    24061 agcagttggt caccatgcta tttgatggag tgctgagcgc actggttaga gcgcgactgt
    24121 ttatgcagga caacaatcag caaggcaaag gcgtctcttt gtcaaaagcg atcaacatca
    24181 ttgagaacgg actgcgggtg agtcttgatg aagagagcaa agacgaatta acccaaaact
    24241 taattgctct ttatagctat atggtcaggc gtttgctgca agccaattta cgcaacgatg
    24301 tctcagcagt cgaagaggtg gaagcattaa tgcgtaatat tgccgatgcc tggaaagagt
    24361 ctttactctc cccttcttcg attcaggacc cagtctgatg aacaatgcac cgcatttgta
    24421 tttcgcctgg caacaactcg tcgaaaaaag ccagctcatg ttacgcctgg caacggaaga
    24481 acaatgggac gaactcatcg ctagcgaaat ggcgtatgtg aatgcggtgc aggagatcgc
    24541 acacctgact gaagaggttg ccccgtccac tacgatgcag gagcagctcc gcccgatgct
    24601 gcacctgatt ctcgacaacg aaagcaaggt aaagcagtta ttacagattc ggatggatga
    24661 actggcgaaa ctggtcggtc agtcatcggt gcaaaaatcg gtgttaagtg cctatggcga
    24721 tcagggcggc tttgtgctgg ctccgcagga taacctcttt tgaatcttaa tgagccgatg
    24781 gctcgcgaat aatccgatta cggctacgct tctaatattc cccttgaatg gagtcgaaga
    24841 atgcgtaatc ccacgctgtt acaatgtttt cactggtatt acccggaagg cggtaagctc
    24901 tggcctgaac tggccgagcg cgcagacggt tttaatgata ttggtatcaa tatggtctgg
    24961 ttgccgcccg cctataaagg cgcatcgggc ggctattcgg tcggctacga ctcctatgat
    25021 ttatttgatc taggtgagtt tgatcagaaa ggcagcatcc ctactaaata tggtgataaa
    25081 gcacaactgc tggccgccat tgacgccctg aaacgtaatg acattgcggt gctgctggac
    25141 gtggtagtca accacaaaat gggcgcggat gaaaaagaag ctattcgcgt gcagcgtgta
    25201 aatgctgatg accgtacgca aattgacgaa gaaatcattg agtgtgaagg ctggacgcgt
    25261 tacaccttcc ccgctcgtgc cgggcaatac tcgcagttta tctgggattt caaatgtttt
    25321 agcggtatcg accatatcga acaccctgat gaagatggca tttttaaaat tgttaacgac
    25381 tataccggag aaggctggaa cgatcaggtt gatgatgaac tcggtaattt cgattatctg
    25441 atgggtgaga atatcgattt tcgcaatcat gccgtgacgg aagagattaa atactgggcg
    25501 cgctgggtga tggaacaaac gcaatgcgac ggctttcgtc ttgatgcggt caaacatatt
    25561 ccggcatggt tttataaaga gtggatcgaa cacgtacagg aagttgcgcc aaagccgctg
    25621 tttattgtgg ctgaatgctg gtcgcatgaa gtcgataaac tgcaaacgta tattgatcag
    25681 gtggaaggca aaaccatgct gtttgatgcg ccgctgcaga tgaaattcca tgaagcatcg
    25741 cgcatgggtc gcgactacga catgacgcag atcttcactg ggacgctggt ggaagccgat
    25801 ccttttcacg ccgtaaccct cgttgccaac cacgacaccc aaccgttgca agccctcgaa
    25861 gcaccggtcg aaccgtggtt taaaccgctg gcgtatgcct taattttgtt gcgggaaaat
    25921 ggcgttcctt cggtgttcta tccggacctc tacggtgcac attacgaaga tgtcggtggt
    25981 gacgggcaaa cctatccgat agatatgcca ataatcgaac agcttgatga gttaattctc
    26041 gcccgtcagc gtttcgccca cggtgtacag acattatttt tcgaccatcc gaactgcatt
    26101 gcctttagcc gcagtggcac cgacgaatat cccggctgcg tggtggtcat gtcgaacgga
    26161 gatgatggcg aaaaaaccat tcatctggga gagaattacg gcaataaaac ctggcgtgat
    26221 tttctgggga accggcaaga gagcgtagtg acagacgaaa acggcgaagc gaccttcttt
    26281 tgcaacggcg gcagcgtcag cgtgtgggtt atcgaagagg tgatttaaat ccatccccgg
    26341 cggcaagccg gggagatttc attacggcag tggcgtcggt aacggcgctt tatccagcgc
    26401 ggcggcacac tctgccgtcg ggcgatccac acgctccatc gtcataccat cgtactcaat
    26461 ggtgttaccg tcacgctcaa cctcgtacag ctcacgtttt acggtaacgt tagtgagatc
    26521 atcagacatc aatgtcaact tacccggcac ggcaatcccc cgctgccact gacggcaatc
    26581 caaggtatcg ccctctttag tgacaatcag gctgcctatc gcttccggac tgaccagcgc
    26641 acgttgcggc cctttggtct gccagtagcc cgccagccag tccggcgcgg gggttttcac
    26701 gacattgtta tagttttcga cttcggcaca gcccgccagc aacatgagcg cgcctgcaat
    26761 tgcgagtttt ttcatcattc gtcctgcatg agaagaaaag aggattgtgg cattaaagct
    26821 ctgatgtcgc cagcctttcc aggttcagag agaaatttga tctaactatt atttttgtga
    26881 attattaccc cgaaaataaa tttgcatggt gtatgattcg cggctcttgt ttcaaacctt
    26941 ctgagttcag aggctacatt catgtcatgg cagcaattca aacacgcctg gttgattaaa
    27001 ttctgggcac ccatccctgc ggtcatcgcg gcgggtattc tctccactta ctattttggc
    27061 attactggca ccttttgggc tgtcacgggt gaatttaccc gttggggcgg tcagctcctg
    27121 cagctgtttg gcgtccatgc tgaagagtgg ggttatttta aaattatcca tctggaagga
    27181 tcgccattaa cccggatcga cggcatgatg atcctcggta tgtttggcgg ctgctttgct
    27241 gctgcgctgt gggccaacaa tgtcaaacta cgcatgccac gcagccgtat ccgcattatg
    27301 caggccatca ttggcggcat tatcgccggt tttggcgcgc gtctggcaat gggctgtaac
    27361 ctggcggcgt tctttaccgg cattcctcag ttctcgctgc atgcctggtt ctttgccatc
    27421 gccactgcca ttggttcatg gtttggcgcg cgctttaccc tgctgcccat cttccgtatt
    27481 cccgtgaaaa tgcagaaagt ttctgccgcc tcaccgctga cgcaaaaacc ggatcaggcg
    27541 cggcgtcgtt ttcgtctcgg gatgctggtc tttttcggca tgctgggctg ggcgctgctc
    27601 acggcgatga accaaccaaa actggggctg gcaatgctgt ttggcgtcgg ctttggttta
    27661 ctgattgaac gtgcgcaaat ctgctttact tcggcgttcc gcgatatgtg gatcaccgga
    27721 cgtacccata tggcgaaagc aatcattatc ggtatggcgg tgagtgccat cgggatcttc
    27781 agttacgtac agttaggcgt tgaacctaaa atcatgtggg cgggaccaaa tgcggtaatt
    27841 ggtggtttac tttttggttt tggcatcgtg ctggcaggcg gctgcgaaac cggctggatg
    27901 taccgcgcgg tagaaggcca ggtgcactac tggtgggtcg gtctgggcaa cgtgatcggc
    27961 tcaacgattc tggcgtatta ctgggatgat ttcgctccgg cgctggccac cgactgggac
    28021 aaaatcaacc tgctgaaaac ttttggtccg atgggaggcc tgctggtgac atatttgctg
    28081 ctgtttgctg cgctgatgtt gattatcggc tgggaaaaac gcttcttccg ccgtgaggca
    28141 ccgcagactg ctaaggagat cgcatgaaaa atatcgttcc tgattaccgc cttgatatgg
    28201 tgggtgaacc ctgcccttat ccggcagtcg caacccttga ggcgatgccg caattaaaaa
    28261 aaggtgagat cctggaagtg gtgagcgact gtccgcagtc gatcaataat attccactgg
    28321 atgcgcgtaa tcacggctac acggtgctgg atattcagca agacgggccg accattcgtt
    28381 atttaattca gaagtaatcc ctcattcccg ctgtggccca cagcgggaac atcgttacct
    28441 ctgacacata gattttaccc gccccctctc ctattctttt cagtatgatc tcaggaggtt
    28501 gctatgtgtg gacgctttgc ccaatcccaa acgcgtgagg attaccttgc gcttctcgcg
    28561 gaagatattg aacgcgatat tccctacgat cccgaaccca ttggcagata caatgtcgcg
    28621 ccgggaacca aagttcttct gctcagtgaa cgtgatgaac accttcatct ggatccggtt
    28681 ttctggggat atgctcccgg atggtgggat aaaccgccgc tgattaacgc ccgcgtagaa
    28741 actgcctcaa ccagtcgcat gtttaaaccg ctctggcaac atggtcgggc aatctgtttt
    28801 gccgatggct ggtttgagtg gaaaaaagaa ggcgacaaaa aaagccttat tttatctatc
    28861 gcgctgacgg acaacctgtt tttatggccg cgataggtag cataccgttt gagcgtggtg
    28921 acgaagccga aggatttttg atcgtcactg cggcggcaga tcaaggtctg gtagatattc
    28981 atgaccgccg cccgctggtt ctgtcgccag aagctgcacg ggaatggatg cggcaagaca
    29041 ttggtggtaa agaagcctca gaaatcgcta ccagaagctg tgtgccagca aaccagttca
    29101 cctggcaccc tgtgtcgcgc acggtgggta atgtcaaaaa ccagggcgcg gagttaattc
    29161 aacctgtttg ttgacatcct ccacgccctg aatgacgagg acccctgcta cgttcaggct
    29221 gtcgcctgaa tcatttcggt gggttcctgc ttcaacgagc ggcctgactg caccatctct
    29281 ccacaggcaa gaacggcgtg ccccgccgct aaaatgttac gagctccatt tacatcggcg
    29341 ttcgctgtat atccacatac ctggcatctg aatttacttt gtgacaggcg attttctttc
    29401 gctgtatgac cacagcacgc gcaacgctgg cttgtgtacg ctggcggaac agcaagcacc
    29461 tgaccgccac gccagagctg cttgtactca agctggcggc gcatttcata ccagccctga
    29521 tccagtatcg aacggtttaa acctgatttt gcccggacat tgcgccccgg ctggctgacc
    29581 gtacccgctg ctgactttga catgttgctg accttcaaat cctcaatgac tatcattacg
    29641 tggtttttgc tgacggtcgt tgtgactttg tgaaggtagt ccctgcggat atttgcgata
    29701 caggaatgca gtcgctgtat tttgcgtttc tgcttctgcc agttgttgct gaatttgacc
    29761 ttgcggctta actgtcgctg aagtctcgcc agcgtcttct ggtttttctg aaaactgttt
    29821 acaggctcaa agactgtgcc atctgacagc gaggcaagtt tagccacgcc agcatccagc
    29881 ccgaccattg atgctgaagg gtgaaccgga gttgatactt cactttctgc ctgaatactg
    29941 atgtaccact taccgcagga ctggctgaca gtgacatttt tcacaacacc cgtgacctga
    30001 cggctgttcc ggtagcgcat ccagccaagt tttggcagaa aaatacggct gttttcctga
    30061 gcgagcttaa cgccctgcgg atagcggaat gcatcattct gtcaccgctt tttgaatcag
    30121 gggaacgccg agcgcttctg gaaaaagttt ttgcaggcct gctcagggtc tttcagtgac
    30181 tgttgcaatg gctgtgacgg agcatcttta atccattgcg tttcagtggc atttttccac
    30241 tcaaccaatc aggaagccat tttcgcgtaa gggatgtctt tgttaccggc cttatgattc
    30301 tcattctgac gtgccagagt acgactgaaa acgaaacgac atgcgcctgc gaagcgcctc
    30361 atttcacgcg cctgttgacc accgggtctt aaccggaatt tgaatacctg tagtcgtttc
    30421 acagggcaaa ttatactttg gtctgtgaaa aaaactgata tccgacgcgg aaggcactgt
    30481 gttttcctga tgcatgctca ccgggtcttt gttaccaagt accgacgcca gatttttgac
    30541 catgacgcga cagaaaaact acgcacttac ttttcaaatg tatgtgctta ttttgaagct
    30601 gaactggttg aaatggatgg cgaaccagat cacgtccatc tgttaattaa ctatcccccc
    30661 aaactggcga tatccagtct ggtaaacaaa cagcctcaaa ggcgtatcgg gtaggttact
    30721 gcgacgggat cgaccagata ttgctgccag gtattaccgc aaagatgttt tgtggcgtcc
    30781 cggctatttt gccagtagct gcggaggtgc gccaatatcc gtcatccgcc aatacattga
    30841 acaacagcaa acacctggtc agatgaaaaa acgagcctta tatccccgcc ctgaaggacg
    30901 ggcttttacg gtgcactgga taagcctgga gattatctac acctgcatgg acatcattgt
    30961 actatgcccc gtacatgctc catcacatcc tcaacatcaa agtattagag ccttgcaaac
    31021 cattatgcgt ctcagtttgt ctcatctatc ttacgcaaaa aaaatagaac taatatcaag
    31081 cggcgctata ttgcgtatta aataaaacca cctcaattac attaataaat ctaaataaat
    31141 atcatgcaaa taatttccat ggttattttt aggctattta aattaaaaaa ataaattatg
    31201 agtcaatcat aaataacaac ctttaatata atttgactac caaatttata ttcgttcaca
    31261 aaatagtcat gcattgtgtg caatagaaac agttcagata aaaataggga tctacttcac
    31321 aatttaaatg agaaactaaa acttacatct tgaaataatc tcattgattg gatgaatatt
    31381 tgtagtgcag tgacatcatt tttaaataat agttcaaata aaagggctca cgatgaaaaa
    31441 attaacagtg gcaatttctg ctgtagctgc atcagtactg atggcgatgt ctgctcaggc
    31501 agcagagatt tacaataaag acagtaacaa gctggatctg tacggaaaag ttaatgctaa
    31561 gcactacttc tcttctaacg atgcagatga tggcgacaca acttatgttc gtctgggctt
    31621 caaaggcgaa actcaaatca acgatcaact gactggtttc ggtcagtggg aatatgaatt
    31681 caaaggcaac cgtgctgaat ctcaaggttc ttccaaagat aaaacccgtc ttgccttcgc
    31741 tggcctgaaa ttcggtgact acggctccat cgattatggc cgtaactacg gtgtagcata
    31801 cgatatcggt gcatggaccg acgttctgcc agaattcggt ggcgatacct ggacccaaac
    31861 agatgtgttc atgactggtc gcaccacagg tgttgcaacc tatcgtaaca acgacttctt
    31921 tggtctggtt gatggtctga actttgctgc tcagtatcag ggtaaaaatg accgcactga
    31981 cgtaactgaa gctaacggtg atggtttcgg tttctccact acttatgagt atgaaggatt
    32041 cggtgtaggt gcaacctatg ctaaatctga ccgcactaat gatcaggtta tctacggtaa
    32101 caacagcctg aatgcatctg gtcaaaatgc tgaagtatgg gcagctggtc tgaaatatga
    32161 tgcgaacaac atctatctgg ctaccaccta ttctgaaacc cagaacatga ctgtttttgg
    32221 taataaccat attgccaaca aagcacaaaa cttcgaagta gttgcacaat atcagttcga
    32281 cttcggtctg cgtccgtccg ttgcttacct gcaatctaaa ggaaaagact tgggtgcgtg
    32341 gggtgatcag gacctgattg aatatattga tgtaggtgca acctattact tcaacaaaaa
    32401 tatgtccact tttgttgatt acaaaatcaa cctgattgat aagagcgatt tcacgaaagc
    32461 atctggcgtt gctaccgatg atatcgttgc tgtaggattg gtttaccagt tctaatctga
    32521 ttactcaaag atatgttgtg ggaggctttg cctccccaac atataagtgg cgctctcaag
    32581 ccactttcct tagaagcact atcttgcttc tgactatata aattaccctt tattttctgg
    32641 gacgtttcaa cgccccattt ttaataatac tcagtgaaca attaacgtat caattagatt
    32701 taataacaac gatatccgta cctaacctga tatctaatgt cataagcatc cctttaatta
    32761 tcccctcaac ctcttgtaaa tttcccactc ttgagctcca catagcccaa acgccggatg
    32821 caaaaaaaat catacgtgca tctctgaaat tgtgatggag gaagtgagta tcaattatat
    32881 gtgcttataa acgttcccga ttggcacaga agtcaccgga ttgttcaggc tccaataggt
    32941 tgagtatgtc gtatttattt tccaaaataa aaatcgatgc tgttgctcct taataaaata
    33001 cttttactct ctggacgctt ttcttagctc tcttggtgtt atattaaaac gattatggaa
    33061 cctttcagta aaacgagaag gacacttgta accattttcc ctggcaatct cgctgatggg
    33121 ttttactgtc gtttgtatag caggacaacg cattatttaa cctcacgtcg tccaatatac
    33181 tttgaaaact tattccctcg cttgccagac ggcgatgcaa tgtggaaaca gaaatgtaga
    33241 gatatctcgc taccttgttt gctgtccatt ttgttctagg ttcggatagc attaggttat
    33301 aacaacgact tattaatgat tgtttactat ctgataaaag taaacaatta acatgatgtg
    33361 ctcctaatga aagtagaacg cccattgcta agtgttcctg aatttgcgtt gagaagcctc
    33421 gggaaataga tgtttttagt tgctcccaac aatatattaa ctcggggttc tgaggtaagc
    33481 agaaacctgt cgtgttacga atttgatcag ttatcgcata aaatttttga aacttttcaa
    33541 ttagatcaat gggataataa agcatttctg caagataaag acctgcttct ggataattca
    33601 caatataaaa ttcatatcca cagggaaata atattatctg attattatca acagttagac
    33661 tatgcgtctc ccaattgata actttttttc cctgacggat acgacataaa gctggcataa
    33721 gaggcttaac cctgtgaatc tcatgatgtt tatgcatccg tatttcttca atctttaggt
    33781 tagacttatc tcttgccagc atactcttac cctattttat ctcataaact ggtgttatct
    33841 cagcggttgc aattttattg gcattaagca tataaccaac taacgctccg ctggaattag
    33901 aatccacagg aatcttatca gtttttagag cccatacttt aaactggtaa tgatggggtt
    33961 tatctccttt gggaggacat gctccaccaa atcctgcata accaaaatca tttcggcctt
    34021 gaacagcacc agtcggaagt tttgtaccat cacgtcttcc tgcatcagtg ggcaaatatg
    34081 ttacagttgc tggaatatta gcaacagtcc agtgccacca tccactgcct gtaggagcat
    34141 ctggatcata cacagttacg gcaaagcttt tggtgccttc aggagcacca gaccaggtta
    34201 atgagggcga tgtattacca ccttcacacc caaatccaga aaatacatga gacgttgtaa
    34261 gttgctctcc tgtttttatt tcattactag tgacctgaaa tgctgcggcc tgcgcagaaa
    34321 atgttatgaa tgccaataca cttgaaacga taagtttttt cataaaaacc tcattgttgt
    34381 gacctatcgt tattttattt gatgtgcctt gatctcatta tgcataaagg cgcaatgatc
    34441 gcgcaaatgc aatcataatt ataaaggtgc cccgaacatt agccataata tgcacaaatc
    34501 agattcctgt tatctcaagg agaattattt tttaatgcgg tgcacttcgt gacaacaagt
    34561 taatgactaa cacaccggca caaatcaaca tcatgcctat gatagctggc aggtccagtc
    34621 gttgaccaaa aaatccccag gaaagcaaac taatcagaac gataccgact cctgaccaga
    34681 tagcataagc aatccctgta ggtatataag ccagcgtctg agctaataac cagaatgatg
    34741 cacaatagca aataattgtg ccaacagagg gccataaccg tgtaaacccc tctgaaaact
    34801 tcattaaggt tgtaccaatg acctctgcaa gtattgcgcc accaagataa atataagggt
    34861 tcatagcata ttctttcctg ttcatactgg agagaattgt actacagttt gaacacaact
    34921 caccgttttc atcctggtgt atgtgtatcc aaggggctct ggcgtggcaa gagataaagg
    34981 caggaacaat aatgatcaga aggaacccca ggctaacgaa tgagttcact cttcctggga
    35041 gcaaagcgaa gacaaaacat gttatcgcgt ttacggatcg tggatggcag aaaacaacca
    35101 ggatcaggta tccattttaa accagaaatt gagtgagttt gccccatcca tgccccacgc
    35161 tgtgggatcg gatataatta aacaagcata gtcaacaggt tacacaacct acacctgcat
    35221 ggacatcact tcctgatacg ccgccaccag cttattacgc acctgaatcc ccatttgcat
    35281 agaaactgag gctttttgca tatcggtcat cacatcgttt agcgccacgc cgggttcacc
    35341 gagggtgaat ttttctgcct gcgtgcgggc gactgtttgt gtatcgctta tgcgatcgag
    35401 cgcggcgtgc agctgcccgg caaaactaat ggtcggttgc ggcagtgatt cctgcgcacg
    35461 cgcactcatc gccgtagcct gtaactggct gataacccct tcaatcccct gtatcgctga
    35521 cattttcgtc tcccggataa tttctggtaa caaagcctac cagtaagtca ataagacaaa
    35581 ggcgctaaat agcaacaaaa aaactggttt attggcggat agaaaaaacg aaagcacaaa
    35641 taatgggagc gtcaattttt cgagtgtgct gacccgagag tgagtcttgt tccactttgc
    35701 caataacgcc gtccataatc agccacgaag tgcgcgatga atgcgactgc agcccagaca
    35761 aaatctcttg agtggcttaa tcgcctgcgt gcgaatccga aaattccatt gattgttacc
    35821 ggttccgcgg cagtggcggt catggtcgca ctgatcctgt gggcgaaagc ccccgactac
    35881 cgcacattat tcagcaatct ttccgatcag gatggtggcg caattgtcag ccaactgacg
    35941 caaatgaata ttccttaccg cttcagcgaa gccagcggcg ctattgaggt tccggcagat
    36001 aaagttcacg aactgcgcct ggcgcaacaa ggtttgccca aaggcggcgc ggtcggtttc
    36061 gaactgctcg atcaggaaaa gtttggtatc agccagttca gcgaacaggt gaattatcag
    36121 cgggcgctgg aaggcgagct ttctcgtacc atcgaaacta tcggcccggt aaaaggggcg
    36181 cgcgtacatc tggcaatgcc gaaaccgtct ttattcgtcc gtgaacaaaa atccccttct
    36241 gcatcggtga cggtaaatct gttacccggc cgcgcactcg atgaagggca aattagcgct
    36301 attgtacatc tggtttccag cgcggttgct ggtctgccgc ctggaaacgt cacgctggtg
    36361 gatcagggcg ggcatctgtt aacccagtcc aataccagcg ggcgcgatct taatgacgct
    36421 cagttgaaat atgccagcga tgtcgaaggc cgtattcagc agcgtattga agcgatcctg
    36481 tcacctattg ttggtaacgg taatattcac gcctaggtca cggcgcaact ggacttcgcc
    36541 agtaaagaac aaacggaaga acagtatcgc cctaacggtg atgaatctca tgcggcgctt
    36601 cgttcacgcc agcttaatga gagcgagcaa agcggttccg gttatccggg cggcgtaccg
    36661 ggggcgttgt cgaatcaacc ggcacctgcg aataacgcgc caatcagcac gcctccgaca
    36721 aatcaaaata accgccagca gcaggcgagc accaccagca atagtgggcc gcgtagcaca
    36781 cagcggaatg aaaccagtaa ctacgaagtc gatcgcacca ttcgtcatac caaaatgaac
    36841 gtgggcgatg tgcaacgtct gtcagtcgcg gtagtggtga attacaaaac cttgccagac
    36901 ggtaaaccat tgcctctcag caacgaacag atgaagcaaa ttgaagctct gacccgcgag
    36961 gcgatgggct tttctgaaaa acgcggtgat tcgctcaatg ttgttaactc gccgttcaat
    37021 agcagtgacg aaagcggcgg agcactgccg ttttggcaac agcaagtgtt tatcgatcag
    37081 ttgctggctg ccggtcgctg gttgctggtt gctggtactg ctggtggcgt ggttgctgtg
    37141 gcggaaagcg gtacgcccgc agctaacacg tcgcgccgag gcggtgaaga ctgtgcagca
    37201 acaggcgcag gcccgcgagg aagtggaaga tgcggtggaa gtccgtctga gcaaagacga
    37261 acaactccaa caacggcgcg ctaaccaacg actgggggca gaagtcatga gccagcgtat
    37321 ccgtgaaatg tctgataacg atccgcgcgt ggtggcgctg gtcattcgcc agtggataaa
    37381 taacgatcat gagtaacctg acaggcaccg ataaaagcgt catcctgctg atgaccattg
    37441 gcgaagaccg ggcggcagag gtgttcaagc acctctccca gcgcgaagtg caaaccctga
    37501 gcgctgcaat ggcgaacgtc acgcagatct ccaacaagca gctaaccgat gtgctggcgg
    37561 agtttgagca agaagctgaa cagtttgctg cactgaatat caacgccaac gattatctgc
    37621 gttcggtatt ggtcaaagct ctgggtgaag aacgtgccgc cagcctgctg gaagatattc
    37681 tcgaaacccg cgataccgcc agcggtattg aaacgctcaa ctttatggag ccgcagagcg
    37741 ccgccgatct gattcgcgat gagcatccgc aaattatcgc caccattctg gtgcatctga
    37801 ggcgcgccca ggccgccgat attctggcgt tgttcgatga acgtctgcgc cacgacgtga
    37861 tgttgcgtat cgccaccttt ggcggcgtgc agccagccgc gctggccgaa ctgaccgaag
    37921 tactgaatgg cttgctcgac ggtcagaatc tcaagcgcag caaaatgggc ggcgtgagaa
    37981 cggcagccga aattatcaac ctgatgaaaa cgcagcagga agaagccgtt attaccgccg
    38041 tgcgtgaatt cgacggcgag ctggcgcaga aaatcatcga cgagatgttc ctgttcgaga
    38101 atctggtgga tgtcgatgat cgcagcattc agcgtctgtt gcaggaagtg gattccgaat
    38161 cgctgctgat cgcgctgaaa ggggccgagc agccactgcg cgagaaattc ctgcgcaata
    38221 tgtcgcagcg tgccgccgat attctgcgcg acgatctcgc caagcgtggt ccggtgcgtc
    38281 tgtcgcaggt cgaaaacgaa cagaaagcga ttctgctgat tgtgcgccgt cttgccgaaa
    38341 ctggcgagat ggtgattggc agcggcgagg atacctatgt ctgataatct gccgtggaaa
    38401 acctggacgc cggacgatct cgcgccacca ccggcagagt ttgtgccaat ggtcgagtcg
    38461 gaagaaacca ttattgaaga ggccgaaccc agccttgagc agcaactggc gcaactgcaa
    38521 atgcaggccc acgagcaggg ttatcaggcg gggattgccg aaggtcgcca gcaaggtcac
    38581 gaacagggct atcaggaagg actggcccag gggctggaac aaggtctggc agaggcgaag
    38641 gctcaacaag cgccaattca tgcccggatg cagcaactgg tcagcgaatt tcaaactacc
    38701 cttgatgcgc ttgatagtgt gatagcttcg cgcctgatgc agatggcgct ggaggcagca
    38761 cgtcaggtga tcggtcagac accaacggtg gataactcgg cactgatcaa acagatccaa
    38821 cagttgttgc agcaagaacc gttattcagc gggaaaccac agctgcgcgt acacccggat
    38881 gatctgcaac gtgtggatga tatgctcggc gcaactttaa gtttgcatgg ctggcgcttg
    38941 cggggcgatc ccaccctcca ccctggcgga tgtaaagtct ccgccgatga aggcgatctc
    39001 gacgccagtg tcgccactcg ctggcaagaa ctctgccgtc tggcagcacc aggagtggtg
    39061 taatgaccac gcgcctgact cgctggctaa ccacgctgga taactttgaa gccaaaatgg
    39121 cgcagttgcc tgcggtacgt cgctacgggc gattaacccg cgctaccggg ctggtgctgg
    39181 aagccaccgg attacaattg ccgctcggcg caacctgtgt cattgagcgc cagaacggca
    39241 gcgaaacgca cgaagtagaa agcgaagtcg ttggctttaa cggtcaacgg ctgtttttaa
    39301 tgccgctgga ggaagtggaa ggtgtcctgc ccggcgcgcg tgtatatgcc aaaaacattt
    39361 cggcagaagg gctgcaaagc ggcaagcagt tgccgctcgg tccggcgtta ttaggtcgtg
    39421 ttctggacgg tagcggtaaa ccgctcgatg gcctgccctt ccccgatacg acggaaaccg
    39481 gtgcgctgat taccccgcca tttaacccgt tgcaacgtac accgattgaa catgtgctgg
    39541 acaccggcgt gcgcccaatc aatgccctgc ttaccgtcgg acgcgggcag cgtatggggc
    39601 tgtttgccgg gtcgggcgtt ggtaaaagtg tgctgctggg gatgatggca cgctacaccc
    39661 gcgccgatgt cattgtcgtg ggtttgattg gtgaacgtgg gcgcgaagta aaagatttta
    39721 ttgagaatat cctcggtgcc gaaggacgtg cacgctcagt ggtgattgcc gctccggcgg
    39781 atgtttctcc gctcctgcga atgcaaggtg ccgcctatgc cacgcgcatt gccgaagatt
    39841 ttcgcgatcg tggtcagcat gtgttgctga ttatggactc cctcacccgc tacgcgatgg
    39901 cccagcgtga aattgcgctg gcgattggcg aaccacctgc cactaaaggt tatccaccgt
    39961 cggtgtttgc caaattaccg gcactggtcg agcgtgccgg taatggcatt agcggcggcg
    40021 gctcgattac cgcgttttat accgtgctca ccgaaggcga tgaccagcag gaccccattg
    40081 ctgactccgc gcgggcaatc ctcgacggtc acattgtgct gtctcgccga ctggcggaag
    40141 ccgggcacta tccggctatc gatattgaag cgtcgatcag tcgcgcaatg acggcgttga
    40201 tcagtgagca acattacgcg cgagtgcgca ccttcaaaca gctgttgtcg agttttcagc
    40261 gtaaccgcga tctggttagc gtcggcgcgt atgccaaagg cagcgatccg atgctcgata
    40321 aagccatcgc cctgtggccg cagctggagg gctatttgca acaaggcatt tttgaacgcg
    40381 cggactggga agcgtctctc caggggctgg agcgtatttt cccgacagtg tcataaccca
    40441 ggagataacg gcagatggcg gaagaacagc tcaaaatgtt gatcgattat cagaatgaat
    40501 atcgcaataa cctcaatagc gatatgagtg ccgggatgac cagcaaccgc tggatcaact
    40561 atcagcagtt tatccagacg ctggaaaaag ccattactca gcatcgccag caacttaatc
    40621 agtggacgca gaaagttgac attgccctga acagctggcg agaaaagaaa caacgtttgc
    40681 aggcctggca gacactgcag gaacggcaat ccacggcggc actgcttgca gaaaaccgcc
    40741 tcgatcagaa aaagatggat gagttcgccc agcgcgccgc catgaggaaa cctgaatgat
    40801 tcgcttagcg cccttgatta ccgccgacgt tgacaccacc acattgcctg gcggcaaagc
    40861 cagcgatgct gcacaagatt ttctcacgtt gttgagcgaa gcattagcag gcgagacaac
    40921 aactgacaaa gcggcccccc agttgctggt agcaacagat aagcccacga caaaaggcga
    40981 gccgctggtc agcgatattc ttgccgatgc gcaacaagcg gatttactga tccctgtgga
    41041 tgaaacactt cctgtcatca acgacgaaca atccacatca acaccgttaa ccaccgctca
    41101 gacgatgacg ttggctgcgg tggctgacaa aaatacgaca aaagacgaaa aagcggatga
    41161 tcttaatgaa gacgtcaccg caagtctgag cgcccttttt gcgatgttgc cgggttttga
    41221 caatacgccc aaagtgactg atgcaccgtc aaccgtgtta ccggcagaga aaccaacgct
    41281 cttcacaaaa ctgacttctg cgcaactcac tacagcacag cctgatgacg cccccggcac
    41341 accagctcag ccattaacac cgctggtagc agaagcccag agtaaagcgg aagtcatcag
    41401 cacgccttcg ccggtgaccg ctgacgccag cccgctaatc actccacacc agacacagcc
    41461 actgcccacc gtcgccgcgc ctgttttgag tgcaccgctg ggttctcacg aatggcaaca
    41521 atcattgagc cagcatattt cgctgttcac ccgccagggg caacaaagtg cagagttgcg
    41581 tctgcaccca caggatttag gtgaagtgca aatttccctc aaagtggatg ataaccaggc
    41641 gcagatccag atgatttcac cgcatcagca cgtacgcgcc gccctggaag cagcgctgcc
    41701 ggtactgcgt acgcagctgg ccgaaagtgg cattcagtta gggcaaagca acattagtgg
    41761 cgaaagcttt agtggtcagc agcaggccgc ttcccagcaa cagcaaagcc aacgcacagc
    41821 aaaccatgaa cctctggcgg gggaagacga cgatacgctt ccggttcccg tctctttaca
    41881 agggcgtgta acaggtaaca gcggcgttga tattttcgcc taacgtcaga ggtagcaccg
    41941 taatccgcgt cttttccccg ctttgttgcg ctcaagacgc aggataatta gccgataagc
    42001 agtagcgaca caggaagacc gcaacacatg actgattacg cgataagcaa gaaaagcaag
    42061 cgatcgcttt ggatcccgat tctggtattc attaccctcg cggcctgtgc cagcgcaggt
    42121 tacagctact ggcattcgca tcaggttgcc gctgacgaca aagcgcagca acgcgtcgtg
    42181 ccctccccgg tcttctatgc gctggatacc tttacggtca atttgggcga tgcggatcgc
    42241 gtgctttata tcggcataac cctgcgactg aaagatgaag caacccgctc gcggctgagt
    42301 gagtatttgc cggaagtccg tagtcgcttg ctgttactgt tttcacgtca ggatgctgcc
    42361 gtactggcga cagaagaagg caagaaaaac ctgattgccg agattaaaac cacactttcc
    42421 accccgcttg ttgccgggca accgaaacag gatgtcaccg acgtgctgta taccgctttt
    42481 attctgcgat aacgacatgg gcgatagtat tctttctcaa gctgaaattg atgcgctgtt
    42541 gaatggtgac agcgaagtca aagacgaacc gacagccagt attagcggcg aaagtgacat
    42601 tcgtccgtac gatccgaata cccaacgacg ggttgtgcgc gaacgtttgc aggcgctgga
    42661 aatcattaat gagcgctttg cccgccattt tcgtatgggg ctgttcaacc tgctgcgccg
    42721 tagcccggac attaccgtcg gggccatccg cattcagccg taccatgaat ttgcccgcaa
    42781 cctgccggtg ccgaccaacc tgaacctaat ccacctgaaa ccgctacgcg gcacagggct
    42841 ggtagtgttc tcgccgagtc tggtgtttat cgccgtggat aacctgtttg gcggcgatgg
    42901 acgcttcccg accaaagtgg aaggtcgcga gtttacccat actgaacagc gcgtcatcaa
    42961 ccgtatgttg aagctggcgc tggaaggcta tagcgacgcc tggaaagcga ttaatccgct
    43021 ggaagttgag tacgtgcgtt cggaaatgca ggtggaattt accaatatca ccacctcgcc
    43081 gaacgacatt gtggttaaca cgccgttcca tgtggagatt ggcaacctga ccggcgaatt
    43141 taatatctgc ctgccattca gcatgatcga gccgctacgg gaattgttgg ttaacccgcc
    43201 gctggaaaac tcgcgtaatg aagatcagaa ctggcgcgat aacctggtgc gccaggtgca
    43261 gcattcacag ctggaactgg tggccaactt tgccgatatc tcgctacgcc tgtcgcagat
    43321 tttaaaactg aaacccggcg acgtcctgcc gatagaaaaa cccgatcgca tcatcgccca
    43381 tgttgacggc gtcccggttc tgaccagtca gtacggtact ctcaacggtc agtatgcgtt
    43441 acggatagaa catttgatta acccgatttt aaattctctg aacgaggaac agcccaaatg
    43501 agtgacatga ataatccggc cgatgacaac aacggcgcaa tggacgatct gtgggctgaa
    43561 gcgttgagcg agcaaaaatc aaccagcgaa aaaagcgctg ccgacgcggt gttccagcaa
    43621 tttggcggtg gtgatgtcag cggaacgttg caggatatcg acctgattat ggatattccg
    43681 gtcaagctga ccgtcgagct gggccgtacg cggatgacca tcaaagagct gttgcgtctg
    43741 acgcaagggt cagtcgtggc gctggacggt ctggcgggcg aaccgctgga tattctgatc
    43801 aacggttatt taatcgccca gggcgaagtg gtggttgttg ccgataaata tggcgtgcgg
    43861 atcaccgata tcattacccc gtccgagcgg atgcgccgcc tgagccgtta gtgatgaata
    43921 accatgctac tgtgcaatct tccgcgccgg tttctgctga gccactgctg caggtgagcg
    43981 gcgcactcat cgccattatt gccctgatcc tcgctgctgc ctggctggta aaacggctgg
    44041 gatttgcccc taaacgcact ggcgttaacg gtctgaaaat tagcgccagt gcttcgctgg
    44101 gcgcgcgtga acgggtcgtg gtggtcgatg tggaagatgc acggctggtg ctcggcgtta
    44161 ccgcagggca aatcaatctg ctgcataaac ttcccccttc agcaccaacg gaagagatac
    44221 cgcagaccga ttttcagtcg gtcatgaaaa atttgcttaa gcgtggcggg agatcctgat
    44281 gcgtcgtttg ttttctgtcg caccggtcct tctctggctg attacgcccc tcgccttcgc
    44341 gcaactgccg ggtatcacca gccagccgct acctggcggg tatcaccagc cagccgctac
    44401 ctggcggtgg acaaagctgg tcgctcccgg tgcagacgct ggtgttcatc acctcgttga
    44461 cgttcattcc agcgatttta ctgatgatga ccagtttcac ccgcatcatt attgtttttg
    44521 gtttattgcg taacgcgctg ggaacgccct ccgcgccacc taaccaggta ttgctggggc
    44581 tggcactgtt tttgaccttt tttattatgt cgccagtgat cgacaaaatt tatgtcgatg
    44641 cgtaccagcc attcagcgaa gagaaaatct caatgcagga ggcgctggaa aaaggggcgc
    44701 agccgctgcg tgagtttatg ctgcgtcaga cccgcgaggc agatttaggg ttgtttgcca
    44761 gactggcgaa taccggcccg ttgcaggggc ctgaagctgt gccgatgcgc attttgctcc
    44821 cggcctacgt gaccagcgag ttgaaaaccg catttcagat aggcttcacg attttcatcc
    44881 cttttttgat tatcgacctg gtgattgcca gcgtgttgat ggcattgggg atgatgatgg
    44941 ttccccccgc caccattgct ctgcccttta aactgatgct gtttgtactg gtggatggct
    45001 ggcaattgct ggtcggttcg ctggcgcaga gcttttacag ctagagaggc aaaatgacac
    45061 ctgaatcggt catgatgatg ggaactgaag cgatgaaagt cgcgctggca ctggcagccc
    45121 cgctgttgct ggtagcgctg gtcacgggcc ttatcatcag tattttgcag gcagccacgc
    45181 agattaacga aatgacgctg tcgtttattc cgaaaatcat cgccgtattt atcgccatca
    45241 ttattgccgg accgtggatg ctcaatctgt tgctggatta cgtccgcacc ttgttcacta
    45301 acctgccgta tatcatcggg tagcccgtac aatgatgcag gagacaagcg atcaatggct
    45361 ttcctggtta agcctgtact tctggccgtt actgcgcgtg ctggcgctca tttctaccgc
    45421 gccaattctg agcgaacgca gcgtgccgaa acgggtaaaa ctgggtctgg caatgatgat
    45481 cacgttcgcc attgccccat cattacctgc caacgatgtt cctgtttttt cgttctttgc
    45541 tctgtggctg gccgtacagc agatcctgat cggcattgcg cttggtttta ccatgcaatt
    45601 tgcctttgcc gctgtgcgaa ccgctggcga aattatcggt ctgcaaatgg ggctgtcatt
    45661 tgcgacattt gtcgatcctg gcagccatct caatatgccc gttttagcgc gcatcatgga
    45721 tatgctggca ttactgctgt tcctgacatt taacggtcat ttatggttga tttcactgct
    45781 ggtcgatacc tttcacaccc tgccgattgg tagcgaaccg ttgaacagca atgcgtttct
    45841 ggcacccacc aaagcaggga gtttgatttt ccttaacggg ctgatgctgg cgttaccgct
    45901 cattactctg ttgctgaccc tgaatctggc attaggttta ctgaatcgta tggccccgca
    45961 attatccatt tttgttattg gatttccatt aactctgact gtcggcatct ctttaatggc
    46021 ggcattaatg ccgttaattg cgcctttttg cgaacattta ttcagtgaaa tttttaattt
    46081 gctggctgat attattagtg aattaccatt aatataattc cgtaacgttt atcatgctac
    46141 cctaaggatt atccgaaaaa taatatctgc gaacatcttc caggatactc ctgtagcgaa
    46201 atatttgttt taagctcact cacatatcgc aacatttact ttaagacaat tccaggcaaa
    46261 ttatacaaca ctttacggga tagtaagtcc gcctgaaaaa ttgcgagagt ggcgcattag
    46321 gtgacccatg ttgttccgtt tagtcatgat gaaatattca ggtaagggga attatcgtta
    46381 cgcattgagt gagggtatgc catgtcaacg attattatgg atttatgtag ttacacccga
    46441 ctaggtttaa ccgggtatct gttgagtaga ggggttaaaa aaagagaaat caacgacatt
    46501 gaaaccgttg atgaccttgc catagcttgt gattcacagc gtccttcagt ggtgtttatt
    46561 aatgaggact gtttcatcca cgatgcttct aacagtcagc gcatcaagca catcattaat
    46621 caacatccca atacgttatt tatcgttttt atggcaattg ccaatgttca ttttgatgaa
    46681 tatctattgg tcagaaaaaa atttattgat cagttctaaa tcgattaaac cggaatctct
    46741 cgacgatatc cttggcgata ttctgaaaaa agagacaacg ataacctcgt ttttaaatat
    46801 gccgacgtta tcattgagcc gaaccgaatc gagtatgttg cgaatgtgga tggcaggtca
    46861 gggaaccatt caaatctccg accaaatgaa tatcaaagcc aagaccgttt catcgcataa
    46921 aggtaatatt aaacgtaaga tcaaaacgca taataaacag gttatctacc atgtcgtccg
    46981 actgacggat aatgtgacta atggtatttt tgtcaacatg cgctaacaca ttctgactgg
    47041 tggtttccca ccagtcaggc tgaataagat tactctgctt tctcaacaaa gataccgtcc
    47101 tgatgccctg cttcattaaa gaaccagatg ccgagcgggt agtcttccag cgcaaccagg
    47161 tacattgtgc cttcactaaa ctcctcaact gccagtacca ctccaggacg acgcggaccg
    47221 ccatccgttt tgactgttac ccgatcattc accttcatcg ttttcctcct gtggctttgt
    47281 gccagtgtag aacaatttcg tttttctggc agcgccaagc gcgcgcgagt gctgattttc
    47341 tcgacggtct atacttaaga gatgccaggc ggacttaacg actggcggca acaacagagt
    47401 aacggttgcg aggaaagatg atgaaaaccg caaaagtgta cagcgatacc gcaaaacgtg
    47461 aggtcagcgt cgatgtcgat gccctgctgg cggcgatcaa tgaaattagc gaaagcgaag
    47521 ttcatcgcag ccagaacgat tctgaacacg ttagcgtcga tggacgtgaa tatcatacat
    47581 ggcgtgaatt ggcggatgcc ttcgaactgg atattcatga cttcagcgtc tctgaagtga
    47641 atcgttgaat gcacaataaa aaaatcccga ccctgagggt gtcgggatga aacttgctta
    47701 agcaagaagc acttaaaaaa ttcgttacac caggaaatct gatgtgttca tcaccttatc
    47761 tgcaattttt ttcgctgaca agaaaatatt cgcgctatga atgattaacg ctacttatga
    47821 atagaaatgt gacgcgcgtc accccttggt ggctctggtg tcaacgggat cgctcccgaa
    47881 aaaatcatct ttgattattt gtgctgtttt gagaatcatc ctggtaaggg gagtactgcg
    47941 cgaccatgtt ttcaattcaa caaccactac tggtttttag cgatcttgat ggcaccctgc
    48001 tggacagtca tagttatgac tggcaaccgg cagccccctg gctcagccgt ttacacgaag
    48061 ccaatattcc cgtcattctc tgtagcagta aaactttagc ggaaatgctg tacttgcaaa
    48121 aaatgttggg gctacaaggc ttaccgctga ttgcagagaa tggcgcagtg atccagcttg
    48181 ctgaacaatg gcaggatata gacggttttc cacgcatcat ctcaggtatt agccatggcg
    48241 aaatctgcca ggttttaaat acgctacgcg agaaagaaca ttttaaattc acgacttttg
    48301 atgatgtcga cgatgcaacc attgccgaat ggacgggatt aagccgtagc taggcggcgc
    48361 tgacgcagct gcatgaggcg tcggtaacgc taatctggcg cgacagtgac gagcgtatgg
    48421 cacaatttat cgctcgtctg aacgaactgg gcttgcagtt tatgcagggt gcgcgcttct
    48481 ggcacgtact ggatgcctct gccggaaaag atcaggctgc caactggatt atcgcgacct
    48541 atcaacaatt gtcaggcaga cgcccaacca cacttggcct gggcgatggg ccaaacgatg
    48601 cgcctttact ggaggtaatg gattacgcgg tgattgtgaa agggctaaac cgtgaagggg
    48661 tacatctgca tgatgaggat ccggcccgcg tctggcgaac gcagcgtgaa ggaccggaag
    48721 gctggcgtga agggctggac cattttttct ccgcccgtta agcgttatcg ctcgcgaata
    48781 cccgattgcg cccggcctgt tttgcgaggt aaagccgacg gtcggccaat gactggagtt
    48841 gttcaaaatc ataatcaccg gtttcctcgc tgctacttac ccccagcgag gcactgatgc
    48901 gtatcgttgt actcttggcg atcagcatct ctttttcatt taacttcagg cgaatacgtt
    48961 ctgcgacctc cgcagcctgc gtcagattcg cgcctggcag aatcacacaa aactcctcac
    49021 caccgacccg cccggcaacg tcctgcgcac gcaaggaact gctaattaat ccggcagcat
    49081 gagaaagaac acggtcgccc gcctgatgac caaagcggtc attaatcgct ttaaaatggt
    49141 caagatcgac ctgaatgaca gaaaaaggat gttggtgcgt ctgacacaat ttagcgagcg
    49201 gacgggcttt ttcgaacagt gcgccacggt tatataaacg cgttaaggtg tcgtgccacg
    49261 cctgccactg caacgagctt tgcagaacat acatgttgct gaccatccgg cgaatcacat
    49321 accaggagag gagtaacatg gtggtaaaga gcgcccacag cagggttaat gcaatgctga
    49381 tactgccgaa atcgccgcgc acgccttcgc ttagcgtatg gacgcgcgcc agcacaccgt
    49441 cgaaatggtc cagacgttcc cagctaacat agcgactgtc catacgaatg ccgccccgcg
    49501 tgtcatgttc cattgcctgc gccagcaagg ccagttcacg aggatcaaaa atattccctg
    49561 ttggatggtc aggattggaa gaggtcaaaa atctcagctt actgtcatag agctgatact
    49621 caccatcgag gtttttatcg atggcgtttc ttaaaaattg ctgcatagta cgcacgggaa
    49681 tactcatccc cagcacgcca taccagtagt tattactgtc taccggaaca ctgacggtaa
    49741 cctgcggttc agtattgctg gcgtgttccg gttgcgaagt gaaccagcgt accgcgcggt
    49801 gacgattttc tcgttgcgaa tggccgataa accagggttg ggtgacatag ccgtaataac
    49861 gcgttggtac attgcgcgta aacaaggtcg gctgcgtcga aacgtaaaat ccagcacgcg
    49921 agacatacat cgcctgttca accatcgacg aggagttgtg cgccagtcgc agcaagtaac
    49981 caacttccag tgcagcggta atttcattgt cgaggctttc attttcgcga gacaggaaat
    50041 tcccctcgct gactaatgca tccgatacgc cattgactgg cagggtgcgc cgtcggttga
    50101 gttcgatttg ccaggcgtgc tcgtcgcgat gctgttcgaa ctcggttacg gcattacgca
    50161 gagaggtgaa atccagtggc gccacgagag cttcgcgcat gccattacgc aaaaagatca
    50221 gtttgtcgac gttatactgc aaatgcttat cgagcgcgtt ggctacgttt tccagatgat
    50281 tacgctagct ggagatatag gcatcttcca gcaccaccac ttcacgccag gtgagcaagg
    50341 tggaaaaaag caataccacg ataaagcaga gattaacgac atgaccgggc cccaggcggc
    50401 gtgcgagttt tttcaaccag ctctggtttt ccatttttgt ctcgtgctgc accctgacga
    50461 ctcccaacct gcttttttat gattctggca taacccggca atagcgtacc gcaaaacagc
    50521 gataatttta taatggtggc atgataagac gcgactatca ttcattgcag gcattgggac
    50581 accaggccgg atgtgatccg gcctggtgtt aatgataccc tttcaaactt cttccgcaga
    50641 acgactcccc tcccccggaa caagatcggt ttcatgaaaa gcttcccgct tgaccccgta
    50701 accgtcatac caacgacact caaccatacc gctggagtat ccagtgacaa tcatccgcgg
    50761 gccgccctct ttaaccgtaa cttcctcact aaccataaag ctcatactcg cctccttttt
    50821 tcgagtgaaa cttgttcacc ttagttgaag atggcgaatt ttgcataata gccagtgcga
    50881 gtattaatgt gcctgaccgc gcattttcgc tactgctttc actcccagca ccacgatgcc
    50941 gccgatgata aatccaagaa tcagatttaa aacagtcggt aatatcatcg ccaccactgc
    51001 actttgctgc ccggcgatat gttcaatagc gtgatgcagc ggcgcaatac catgtaccac
    51061 aatcccgccg ccgacgagga acatcgccag cgtgccgaca atcgataatg ctttcatcag
    51121 ccaaggcgcg ataatcaata accctttacc taatgcctgc atcagcgcgc tagatttttc
    51181 cgccagccaa taccctaggt catcaatctt aacgataacc cctaccagac cgtagacgcc
    51241 cacggtcact accagcgcga tgcctgaaag caccagcacc tgattaagca acggcgcctc
    51301 tgccacaatc cccagcgtga tggcgacgat ttccgcagac aaaataaaat cggtacgaat
    51361 cgccccttta attttgtcct tttcaaactt cagcggatcc tgcgccgcca gcttctccag
    51421 acgctgctgg ctctgcgctg gatcttcttt atgtttacgc gcctccagca tatgcagcac
    51481 tttctctacc ccttcaaagc agagaaacgc gccaccaatc atcaacagtg gcgtaatcgc
    51541 ccacgggata aacgcactga tgatcagcgc cagcggcacc agaatcactt tattgatcag
    51601 cgatcctttc gccacgcccc agaccacggg aagttcccgg ttggcccgca cgcctgaaac
    51661 ttgttgcgca ttgagcgata aatcatcccc taatacaccg gcggttttct tcgccgccag
    51721 tttgcccatc acagagatat cgtccagcag tgtggcgata tcatcgagca acgtcagtaa
    51781 actactcccg gccaaaagca ttccctctca tttttattgt tgaagggtgt aagtatgaag
    51841 caaaaacggc agctctgata cttgcttcac aggtcaacat ttatttaaca atcaagtgca
    51901 attatatctc tcgccagcag aatgattttc acgtttacta tgaaccacct ttttatttcc
    51961 gccgtgaggg attatgcgtt tccgccagtt gttaccgctt tttggcgcgc tgtttgcgtt
    52021 gtatatcatt tggggctcaa cctattttgt cattcggatt ggcgtgggaa gctggcctcc
    52081 gttaatgatg gcgggcgttc gattccaggc agccggtatt ttattgctgg catttttgct
    52141 actgcgcgga cacaaacttc ccccgctacg tccgctgctc aatgccgcgc tgattggcct
    52201 gttattgctg gctgtcggta atggcatggt gacggttgcc gaacatcaaa atgttccttc
    52261 cggcatcgcc gccgtagtga ttgcaaccgt gcccctcttt accctgtgct tcagtcgcct
    52321 gtttggcatt aaaacgcgca aactggaatg ggtgggtatt gccattgggc ttgccggaat
    52381 catcatgctc aatagcggtg gaaatttaag cggcaatccg tggggcgcga ttctgatttt
    52441 aatcggctcg attagctggg cgtttggctc agtttatggc tcgcgcatta ccttacctgt
    52501 agggatgatg gcgggtgcga ttgagatgct ggcggcaggc gtggtgttaa tgatcgcatc
    52561 gatgattgcg ggtgaaaaac tgacggcgct cccttccctg tcaggcttcc ttgcggtcgg
    52621 ctatctggcg ctgtttggtt cgattatcgc cattaacgct tatatgtatt taatccgtaa
    52681 tgtcagtccg gctctcgcca ccagctacgc ctacgttaac ccggtggtcg cggtcttgct
    52741 gggtacggga ctgggtggcg aaacgctgtc gaagattgaa tggctggcgc tcggcgtaat
    52801 tgttttcgcg gtggtactgg tcacgttggg aaaatatctc ttcccggcca aacccgttgt
    52861 cgcgccagtt attcaggatg catcaagcga gtaaatgaat cccctgcgtg tcgatctgcg
    52921 cgctggcacc ttcgccgcag atccactctt ccagacgctc ggtaagcgcc gcatccgtca
    52981 gcttctcgcg cctacgtaac gcacactccc agacaatcag tacgcgccag ccgagctcct
    53041 gcaagcgact gatatcgcgg cgatcgcgtt caacattttt acctatcttc tccagccaga
    53101 actcagttcg agtcgcaggc actttaaaca gatagcagtg atgatgatgc cagaagcagc
    53161 cgtgggtaaa tatcacgcag cgatattcat caacgacaaa atccgggcgt ccgggcagac
    53221 tggcgtcctg aacgcgaaac gcaaggccct gcccggttaa caaactggcg aggcgcttct
    53281 caatcgccgt atcacgcgtg gcaatcgcgc gcatattttt gctgcgggtg gccttatcgt
    53341 gaacgtcgac catgttgtgc ctcttgctga cgcaacgcca ccgcctgttt gatttttggc
    53401 tcaagcagtt ttgccaccgc ggcaaagacc ggcacgacca ccgagttgcc gaactggcga
    53461 taggcctgag tgtccgaaac cggaatgcgg aatttcgctt ctcccggcgc ttcaaaaccc
    53521 attaagcgcg cgcattcacg aggcgttaac cgacgtggac gatgttgctg attcagcgga
    53581 tcgtcaaagt ctttctcacc cgtggccata tcccaaccgc gatcgattaa aatttccgcg
    53641 ccatctttgt aataacgagc agacagcgta cgggtgacgc tttgcggatt gttcggataa
    53701 accattccat aaccgaagcc gttaccgcgc gcctgatgtt ttttcgcata tcgatagagg
    53761 tacttccaca gcaccggcgt caggatatat ttcgcctcaa ccatcgggtc cagcagctgc
    53821 gccagcgtca ctcgctgtgc agggaaacat tcgctgatat cacgcagagt aaaatcggct
    53881 ttcagattca gatcgcgacg aaaacccacc agcacgatgc gttcacggtg ctgcggcagg
    53941 aaatgtttac catcgatgat tttcggatcg tccggcccgt tatcttctgc atcggcaacg
    54001 tcatagccca gttcgtccag cgtctgcatg atgatgcgga acgtttttcc cttatcgtga
    54061 ctcttcaggt ttttgacgtt ttcgagcaca aacatcgccg gacgacgcgc gtcgataatg
    54121 cgcaccacat caaagaacag cgtgccctgg gtatcgcagg caaaaccgtg cgcccgcccg
    54181 agcgagttct ttttcgatac gccagccagc gaaaatggct gacaagggaa accggccagt
    54241 aaaacatcgt gttcaggaat gtgttgacga atatgttccg ccgccgcctc atcactcacg
    54301 ccttcttgat gactgagggt gatgtcgcgg atatcttcat taaaatgatg cgtcgccggg
    54361 tcgcaataat ggttggcttt ataagtgcgt accgcatgtt tgttccattc gctggtaaac
    54421 acgcactgtc caccaatcga ttcaaaacca cgacggatgc cgccaattcc ggcaaataga
    54481 tcgataaagc gaaacgcata atgcggatga tgttccggtg gtttgggtaa caacgtttgc
    54541 agatgggcaa actctttctc acttaaacgg tgccatgccg agtcattcgc cagcgcacgt
    54601 tttaaaattg ccgcgctcca gtgattctca cccacaccat taagctgcgc caccagcatt
    54661 ttaacatcat aaatttgcag cagtttctcc agcattgcct gtgcggcatt cccggtgctg
    54721 tatgaatcgg ttactgatat attttcctgc atagatttaa ccggccatct gagatggccg
    54781 acaggttaac ataattactg ccgtttaggg ccacgcgctc agccagcggg tgaaaacgca
    54841 tctatcacct tgtgatcgat accttcattc tcccccgcca gttcggcacc gagcttcgcc
    54901 atcaaacagc gccaagctca atgcccctga aacggcaaat acgcgcgcca ggccaatcgc
    54961 ccccagggct tccagccgat ccgcatcctg gacgatttta gcttccgttg ttaagggggc
    55021 gatttgcgcg ctgaaactgt gagcggcaat ggcatgacaa acggcctcaa ttttctccgc
    55081 cggaaattgc acgaactctt cacggagcag gcgacgcgtc tcttctgccg ccaggattga
    55141 agaacgctgc cgttgcggat gatttttcgc caggctcacg atatcgtgaa aataacaggc
    55201 ggttaaaatc accagcatat cgacatcgtc atctgccgcc agtttctgtg ccgtagccca
    55261 gacacggcga aaatgacaca ggtcatgcgc cgcgtcctga tgttgatgat ggtttttcaa
    55321 ccagttctcg aactatgcct gccagtgttg taactccata cgcgctccgt tgccaaagag
    55381 tctcaacctt agcagttttt attcatagcg tctgcaacgt gcggaacgag gtcgggtgta
    55441 ccatgcaatg ccgcctaaca aggcaccaac cagggccata aacagaaatc cgaccaatgc
    55501 tcctaaccac ttccccgcgg tagatcctaa atcactttca aatcccgaca cgggggcatc
    55561 ggggatccgg ctcatcaccc agatgtatcg cgccattgcc cagacgaaat agccacacca
    55621 tgtatagaaa gcccac
//
LOCUS       DAJTQQ010000016        55369 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000016 DAJTQQ010000000
VERSION     DAJTQQ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55369
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.016"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(103..1476)
                     /gene="argH"
                     /locus_tag="OO309_001071"
     CDS             complement(103..1476)
                     /gene="argH"
                     /locus_tag="OO309_001071"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR5846289.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1537..2313)
                     /gene="argB"
                     /locus_tag="OO309_001072"
     CDS             complement(1537..2313)
                     /gene="argB"
                     /locus_tag="OO309_001072"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR5846290.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2321..3325)
                     /gene="argC"
                     /locus_tag="OO309_001073"
     CDS             complement(2321..3325)
                     /gene="argC"
                     /locus_tag="OO309_001073"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR5846291.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3479..4630
                     /gene="argE"
                     /locus_tag="OO309_001074"
     CDS             3479..4630
                     /gene="argE"
                     /locus_tag="OO309_001074"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR5846292.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4982..7633
                     /gene="ppc"
                     /locus_tag="OO309_001075"
     CDS             4982..7633
                     /gene="ppc"
                     /locus_tag="OO309_001075"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR5846293.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7816..9549
                     /locus_tag="OO309_001076"
     CDS             7816..9549
                     /locus_tag="OO309_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR5846294.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9698..10549
                     /locus_tag="OO309_001077"
     CDS             9698..10549
                     /locus_tag="OO309_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5846295.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10536..10877)
                     /locus_tag="OO309_001078"
     CDS             complement(10536..10877)
                     /locus_tag="OO309_001078"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5846296.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10879..11757)
                     /locus_tag="OO309_001079"
     CDS             complement(10879..11757)
                     /locus_tag="OO309_001079"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR5846297.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11723..14020)
                     /locus_tag="OO309_001080"
     CDS             complement(11723..14020)
                     /locus_tag="OO309_001080"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5846298.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14071..14391)
                     /locus_tag="OO309_001081"
     CDS             complement(14071..14391)
                     /locus_tag="OO309_001081"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5846299.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14406..15485)
                     /locus_tag="OO309_001082"
     CDS             complement(14406..15485)
                     /locus_tag="OO309_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR5846300.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15794..16897
                     /locus_tag="OO309_001083"
     CDS             15794..16897
                     /locus_tag="OO309_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR5846301.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16898..18295
                     /gene="ptsP"
                     /locus_tag="OO309_001084"
     CDS             16898..18295
                     /gene="ptsP"
                     /locus_tag="OO309_001084"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5846302.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18307..18969
                     /gene="fsa"
                     /locus_tag="OO309_001085"
                     /pseudo
     CDS             18307..18969
                     /gene="fsa"
                     /locus_tag="OO309_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18980..20081
                     /gene="gldA"
                     /locus_tag="OO309_001086"
                     /pseudo
     CDS             18980..20081
                     /gene="gldA"
                     /locus_tag="OO309_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20356..20963
                     /locus_tag="OO309_001087"
                     /pseudo
     CDS             20356..20963
                     /locus_tag="OO309_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(20990..21895)
                     /gene="yijE"
                     /locus_tag="OO309_001088"
     CDS             complement(20990..21895)
                     /gene="yijE"
                     /locus_tag="OO309_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR5846303.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21989..24169)
                     /gene="katG"
                     /locus_tag="OO309_001089"
     CDS             complement(21989..24169)
                     /gene="katG"
                     /locus_tag="OO309_001089"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR5846304.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24498..25388)
                     /gene="metF"
                     /locus_tag="OO309_001090"
     CDS             complement(24498..25388)
                     /gene="metF"
                     /locus_tag="OO309_001090"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR5846305.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25737..28169)
                     /gene="metL"
                     /locus_tag="OO309_001091"
     CDS             complement(25737..28169)
                     /gene="metL"
                     /locus_tag="OO309_001091"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR5846306.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28172..29332)
                     /gene="metB"
                     /locus_tag="OO309_001092"
     CDS             complement(28172..29332)
                     /gene="metB"
                     /locus_tag="OO309_001092"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR5846307.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29609..29926
                     /gene="metJ"
                     /locus_tag="OO309_001093"
     CDS             29609..29926
                     /gene="metJ"
                     /locus_tag="OO309_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR5846308.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30110..30718
                     /locus_tag="OO309_001094"
     CDS             30110..30718
                     /locus_tag="OO309_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR5846309.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30779..30991)
                     /gene="rpmE"
                     /locus_tag="OO309_001095"
     CDS             complement(30779..30991)
                     /gene="rpmE"
                     /locus_tag="OO309_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR5846310.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31194..33392
                     /gene="priA"
                     /locus_tag="OO309_001096"
     CDS             31194..33392
                     /gene="priA"
                     /locus_tag="OO309_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR5846311.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33548..34573
                     /gene="cytR"
                     /locus_tag="OO309_001097"
     CDS             33548..34573
                     /gene="cytR"
                     /locus_tag="OO309_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR5846312.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34665..35624
                     /gene="ftsN"
                     /locus_tag="OO309_001098"
     CDS             34665..35624
                     /gene="ftsN"
                     /locus_tag="OO309_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR5846313.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35717..36247
                     /gene="hslV"
                     /locus_tag="OO309_001099"
     CDS             35717..36247
                     /gene="hslV"
                     /locus_tag="OO309_001099"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR5846314.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36257..37588
                     /gene="hslU"
                     /locus_tag="OO309_001100"
     CDS             36257..37588
                     /gene="hslU"
                     /locus_tag="OO309_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR5846315.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37655..38581
                     /gene="menA"
                     /locus_tag="OO309_001101"
     CDS             37655..38581
                     /gene="menA"
                     /locus_tag="OO309_001101"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR5846316.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38674..39159
                     /gene="rraA"
                     /locus_tag="OO309_001102"
     CDS             38674..39159
                     /gene="rraA"
                     /locus_tag="OO309_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR5846317.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39244..39489)
                     /gene="zapB"
                     /locus_tag="OO309_001103"
     CDS             complement(39244..39489)
                     /gene="zapB"
                     /locus_tag="OO309_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR5846318.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39915..40760
                     /gene="glpF"
                     /locus_tag="OO309_001104"
                     /pseudo
     CDS             39915..40760
                     /gene="glpF"
                     /locus_tag="OO309_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40800..42290
                     /gene="glpK"
                     /locus_tag="OO309_001105"
     CDS             40800..42290
                     /gene="glpK"
                     /locus_tag="OO309_001105"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR5846319.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42520..43530
                     /gene="glpX"
                     /locus_tag="OO309_001106"
     CDS             42520..43530
                     /gene="glpX"
                     /locus_tag="OO309_001106"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5846320.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43627..44373
                     /gene="fpr"
                     /locus_tag="OO309_001107"
     CDS             43627..44373
                     /gene="fpr"
                     /locus_tag="OO309_001107"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR5846321.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44378..44806)
                     /gene="uspD"
                     /locus_tag="OO309_001108"
     CDS             complement(44378..44806)
                     /gene="uspD"
                     /locus_tag="OO309_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR5846322.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44833..45132)
                     /locus_tag="OO309_001109"
     CDS             complement(44833..45132)
                     /locus_tag="OO309_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR5846323.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45344..45784)
                     /locus_tag="OO309_001110"
                     /pseudo
     CDS             complement(45344..45784)
                     /locus_tag="OO309_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45885..46484
                     /locus_tag="OO309_001111"
     CDS             45885..46484
                     /locus_tag="OO309_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR5846324.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46592..47359
                     /gene="tpiA"
                     /locus_tag="OO309_001112"
     CDS             46592..47359
                     /gene="tpiA"
                     /locus_tag="OO309_001112"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR5846325.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47414..48169)
                     /gene="cdh"
                     /locus_tag="OO309_001113"
     CDS             complement(47414..48169)
                     /gene="cdh"
                     /locus_tag="OO309_001113"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR5846326.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48276..49265)
                     /gene="sbp"
                     /locus_tag="OO309_001114"
     CDS             complement(48276..49265)
                     /gene="sbp"
                     /locus_tag="OO309_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR5846327.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49585..50547)
                     /gene="pfkA"
                     /locus_tag="OO309_001115"
     CDS             complement(49585..50547)
                     /gene="pfkA"
                     /locus_tag="OO309_001115"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR5846328.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50728..51630)
                     /gene="fieF"
                     /locus_tag="OO309_001116"
     CDS             complement(50728..51630)
                     /gene="fieF"
                     /locus_tag="OO309_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR5846329.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51778..52278)
                     /gene="cpxP"
                     /locus_tag="OO309_001117"
     CDS             complement(51778..52278)
                     /gene="cpxP"
                     /locus_tag="OO309_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR5846330.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52428..53126
                     /gene="cpxR"
                     /locus_tag="OO309_001118"
     CDS             52428..53126
                     /gene="cpxR"
                     /locus_tag="OO309_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR5846331.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53123..54496
                     /gene="cpxA"
                     /locus_tag="OO309_001119"
     CDS             53123..54496
                     /gene="cpxA"
                     /locus_tag="OO309_001119"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR5846332.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54602..55276)
                     /gene="yiiM"
                     /locus_tag="OO309_001120"
     CDS             complement(54602..55276)
                     /gene="yiiM"
                     /locus_tag="OO309_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR5846333.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13480 a        15006 c        14149 g        12734 t
ORIGIN      
        1 aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg cactttgtag
       61 gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta accgagcctg
      121 cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg ccgcacgctt
      181 ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca cctgactgaa
      241 tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa tggcttccac
      301 caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg ccaccaggta
      361 atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac aacgcggacg
      421 tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca gccaggtatc
      481 gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag ccagcggcaa
      541 acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc atttaccgcg
      601 aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac cggaagtcac
      661 gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca gatcttcagc
      721 aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca acacatggtc
      781 acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca gccagcctgc
      841 taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc ctaatgggct
      901 gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca gcatctcaac
      961 ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt gagtgtaacc
     1021 tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt gcagctgccg
     1081 gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt cagtcgctac
     1141 ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca ctttgtcgat
     1201 cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga tttgttgtgg
     1261 cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg cctgctcttc
     1321 tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc caacaatatc
     1381 ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga accgttgatc
     1441 tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt attttgaaat
     1501 tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc gcgtacccat
     1561 cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag cgatatctac
     1621 cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta tcatgccgtc
     1681 agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt cggcaatgcg
     1741 ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat ccgcgcccag
     1801 cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt gcccttcgtc
     1861 tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg agttgataag
     1921 cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa gctgggtcac
     1981 tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat gtttcttcgc
     2041 ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa tgtctatctg
     2101 atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca gccctttcat
     2161 cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct gatgtgactc
     2221 acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat ccagcagtac
     2281 gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga gactgcgttt
     2341 ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg cctttcagta
     2401 agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac ccgatatcgc
     2461 aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac agccgcacca
     2521 gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg gtcacacccg
     2581 atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc agatgtgggg
     2641 tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga tgagtaaaga
     2701 cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc ttacgtcctg
     2761 caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca agaagatcgg
     2821 catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct ggcaccgcaa
     2881 tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag gctgcctgtt
     2941 ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag gtggcgtcgt
     3001 taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga aactgcggcg
     3061 ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc cctgggctaa
     3121 actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt agctgcggat
     3181 gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc aaagcggtta
     3241 tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg tagccgctgg
     3301 cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct ggttgccagg
     3361 ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac gttagtgtat
     3421 ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg tgtcaacaat
     3481 gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca caccttcaat
     3541 aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc tgctggcgga
     3601 ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa ctcgcaacaa
     3661 atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg cggggcatac
     3721 cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac tgacggagca
     3781 tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg cgtttatcct
     3841 tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca ttctggcgac
     3901 tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta ccgccctgcg
     3961 cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg cacataaagg
     4021 tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg atccagcacg
     4081 cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc aattgcgcga
     4141 taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta cgctcaacct
     4201 cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg agttgcatat
     4261 ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc tcaacgatgc
     4321 attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc tgcatccgcc
     4381 gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg agaaattgct
     4441 cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc aaacgttatg
     4501 cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg atgaatatct
     4561 ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc accatttttg
     4621 ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc gttgccaaac
     4681 tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc tacacctttg
     4741 gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa agcgtcgtga
     4801 atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg cagcatttga
     4861 cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg agcatattcg
     4921 cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg tctggggtaa
     4981 tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca aagtgctggg
     5041 agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa ctatccgtaa
     5101 gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc tcaccacctt
     5161 acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc agttcctgaa
     5221 cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag ctgccagcaa
     5281 cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac tgagcgaaga
     5341 caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg ctcacccaac
     5401 cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct gtttaaaaca
     5461 gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc gcctgcgcca
     5521 gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa gcccggtaga
     5581 tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg taccgaatta
     5641 cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc ccgtcgaatt
     5701 tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc cgaacgtcac
     5761 tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg atctgttcct
     5821 gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc ctgaactgct
     5881 ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga aaaacctgcg
     5941 ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg aagaactgcc
     6001 aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct acgcttgcta
     6061 ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc tcgacaccct
     6121 gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc aggagagcac
     6181 ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg actacgaaag
     6241 ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca aacgtccgct
     6301 tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata cctgccaggt
     6361 gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg cgaaaacgcc
     6421 gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt ttgcgatgcc
     6481 ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg tcatgaccca
     6541 gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg tgatgattgg
     6601 ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc aatatcaggc
     6661 acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt tgttccacgg
     6721 tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc tgtcacaacc
     6781 gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga tccgctttaa
     6841 atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg cgattctgga
     6901 agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta tggatgaact
     6961 gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag attttgtgcc
     7021 ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg gttcacgtcc
     7081 ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt ggatttttgc
     7141 ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg cgctgcaaaa
     7201 agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt ggccattctt
     7261 ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt ggttggcgga
     7321 atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag agttacgcaa
     7381 cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc atctgatggc
     7441 cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg acccgctgaa
     7501 cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag gccaggaacc
     7561 ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg caggtatgcg
     7621 taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag ggttttctga
     7681 aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt tattcaaatt
     7741 ctgaatatac cttcagatat ccttaaggaa ttgtcgttat attcggcgat atttttttca
     7801 agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc tagctggaaa
     7861 gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca ggccattatt
     7921 tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt cagctcgctg
     7981 tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc agtgatcggc
     8041 gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta cgggcaggag
     8101 ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc cagcgagtat
     8161 ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac ggcggtggca
     8221 gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg ttatgttgtc
     8281 tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac gtttatcaaa
     8341 aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc tgccgcaccg
     8401 tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct aacaaagtta
     8461 ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc gggtaacgaa
     8521 ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat gagtctgtac
     8581 ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga tccgaatctg
     8641 accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct gcaacaggcg
     8701 ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc gctgatgaac
     8761 atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac gatgaccgcc
     8821 cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta catgaaccag
     8881 caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt ccaggatgtg
     8941 ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac gcatatcaaa
     9001 tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga tcatgttccg
     9061 ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc taacctgtat
     9121 aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc gaacggattc
     9181 ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca caaaactcag
     9241 gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct gctgtggacg
     9301 tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga tcgtaaatac
     9361 agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta cgacggttac
     9421 gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg gattggtaac
     9481 ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga tcaggtgggt
     9541 aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga tactgccgca
     9601 atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt aataatgcca
     9661 gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg tcagctatct
     9721 gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg ccagcagtaa
     9781 cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa ttgtgctgga
     9841 aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg tgctgtacgt
     9901 tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct ttagcgtgct
     9961 gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac aatatcaaaa
    10021 tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc tgctacaaac
    10081 gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta tcgtagccag
    10141 cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct cacgcagcca
    10201 ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg cgcttacccg
    10261 cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc tgtttcaaaa
    10321 aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc acgctaagac
    10381 gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg gctttgtcga
    10441 cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt cggaatatcg
    10501 ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag atgggtttgc
    10561 tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc acgcaggaat
    10621 gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc ggcaagctcg
    10681 atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc taaacgattc
    10741 tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa ctggcacaac
    10801 ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca ggcggttact
    10861 gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc gttctgccat
    10921 ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca tcgaccatgt
    10981 tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca gatggatctg
    11041 cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc ggctgagcgt
    11101 gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca ccagcaaacg
    11161 cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct gagtcgcgtc
    11221 cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta acagcttgct
    11281 tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc gcagtcgctg
    11341 taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa gcgtgacgcc
    11401 gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca gagcgtcaag
    11461 gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg catcacgcaa
    11521 acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt tgccggagat
    11581 cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa agaccaccgt
    11641 acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc gcgccacatc
    11701 atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg atgtcatcct
    11761 ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc accaccagcc
    11821 cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc accacgttaa
    11881 actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc agtttgcgta
    11941 atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac gtaccgttag
    12001 acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc ccttgtgcgt
    12061 cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca aaacgaccgt
    12121 ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta tacgatcccg
    12181 gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag tagtggcgca
    12241 acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc tcaaagcggt
    12301 taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct tttaataccg
    12361 acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc aacgcatgga
    12421 gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta taacgcgcgc
    12481 cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac gagagcagcg
    12541 gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca accatcaggg
    12601 tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa gtcagcgcag
    12661 cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta aacatcgcga
    12721 tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc actacggaat
    12781 agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc accacttcat
    12841 cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt ttcatcagga
    12901 acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt aattgcacgc
    12961 tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt ccgttctcgg
    13021 ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa cgcgcgctac
    13081 tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg agcagttctt
    13141 tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc aacatatact
    13201 ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga atgatgttca
    13261 tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc gcgttatggc
    13321 gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg cagtttgccg
    13381 ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct tccagcagta
    13441 acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc tgcatctgtg
    13501 ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata atgtgcccct
    13561 ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct tttacttcat
    13621 ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac gggaacaact
    13681 cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc ggacgcgtcg
    13741 ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac ataatcccgg
    13801 cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc gaaatttcaa
    13861 catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt tcgccttcgg
    13921 tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca cgggtattgg
    13981 caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc tgtgggaaag
    14041 cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt gagcttcaat
    14101 tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc gcacgatggt
    14161 cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa tcaccatatc
    14221 agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc cctgggtttc
    14281 tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg ctgccatata
    14341 ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca tggctcgttc
    14401 ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc agtcgaagac
    14461 ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac cactgctccg
    14521 acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac aatcagacca
    14581 ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc acaaactgaa
    14641 ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg aatcgcccct
    14701 tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg ctcttctgcg
    14761 gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg gatacaaata
    14821 cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat cagcatgaag
    14881 gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag ccccatgatc
    14941 accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt caggctgttg
    15001 gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt aatcagtgtg
    15061 ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg caccggaatt
    15121 ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc accaaagaag
    15181 cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag cgcagaacgc
    15241 tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt caaacctgca
    15301 acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg cactgcgcct
    15361 ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac gaagggaatc
    15421 atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg caccaactca
    15481 ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg gaggaaacaa
    15541 taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga cttttgtaat
    15601 atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca tgccgcaaaa
    15661 cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc agcttttggc
    15721 agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag aaaaccgtac
    15781 cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta acggcgtaca
    15841 tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc aaattgagtg
    15901 gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc tgattggcac
    15961 cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg aacaggaagg
    16021 gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg cgccgctggc
    16081 ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc tgaatccgca
    16141 aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga cgccgatctc
    16201 ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg acgccgagca
    16261 atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc gtctgctgga
    16321 tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg gcgatacttc
    16381 cctgcacgaa catttactgg caggcgtcag cgccggatta agctgcgccg aagcgattgt
    16441 cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat acctgcaaga
    16501 acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct acggtgagca
    16561 acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg atgaactgac
    16621 ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac tgaaaagcgg
    16681 cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga cgctggttgg
    16741 tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg acggcaacgc
    16801 cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc aagaagcccg
    16861 cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag cccgtaccgc
    16921 tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag cgcaggccgc
    16981 attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct atatggatcg
    17041 caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc tggagtctgc
    17101 caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac ccgttgatta
    17161 tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc gtatttatga
    17221 agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct ccgctcacgg
    17281 cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat gggtgaaaga
    17341 aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg atgagaagat
    17401 ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc aatgctgcga
    17461 agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc tggcagtgga
    17521 tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat tcttgcgggc
    17581 gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc tgtgcggtga
    17641 gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg atgaactcag
    17701 catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg atagccgtga
    17761 gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag tggaacacct
    17821 gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg agtgcatcac
    17881 gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg ataacctgct
    17941 gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg cgcgcgaggc
    18001 cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat cagaacacat
    18061 tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg gcgatgatga
    18121 agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc agcacatgcg
    18181 cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg cgctggttaa
    18241 cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac tgtaaaagga
    18301 aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg tctggcacgc
    18361 atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag caaggagtcc
    18421 atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat tctgtttgct
    18481 cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct gcgcgacgct
    18541 attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc aattaaaata
    18601 ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc agaagggtta
    18661 ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt agatgcccag
    18721 ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat gcacgcgcca
    18781 gaaagcatgg tgctggcagc cagctttaaa acgccgcgtc aggcgctgga ctgtttactg
    18841 gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct caacacccct
    18901 gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt tggcactact
    18961 catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat acatccaggg
    19021 cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac gctggttagt
    19081 ggtgggtgac aaatttgttt tagtttttgc tcaatccact gtcgagaaaa gctttaaaga
    19141 tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa atgagatcga
    19201 ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta tcggtggcgg
    19261 aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg tagcgatcgc
    19321 accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct acaccgatga
    19381 gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca ttgtcgacac
    19441 caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg atgcgctggc
    19501 aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg cgggcggcaa
    19561 gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc tggaagaagg
    19621 cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg agcgcgtgat
    19681 tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc ggcggctcac
    19741 gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca cggtgaaaag
    19801 tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag gaaatcgaaa
    19861 ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa ctggatatta
    19921 aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca gaaggtgaaa
    19981 ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca ctgctggtag
    20041 ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca aactcccggc
    20101 ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg cctacgcaat
    20161 ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc actccgcatc
    20221 cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt tatccgcttc
    20281 tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg actcttattt
    20341 tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac ggcatttacc
    20401 agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca ggccaatacc
    20461 aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt ttacgatcgc
    20521 agcttaccta tcctggcggt gatgttccgc aagaacgcgg tgtatgttcc gatatggtga
    20581 tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa gatatggcga
    20641 agaattttgc tgagtacccg caaaaatgga agttaaagcg cccggacagc aacatcgatc
    20701 accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca cgccccatca
    20761 gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta gataacgggc
    20821 tggcgcatat tggggtggcg tcagatggct tcgcccgcga cggtacgccg ctggtgatac
    20881 ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg atggtggggc
    20941 attatcgtta ttttgttaaa taagagatcg ccccacatga agaaaaaatt cagatgcttt
    21001 ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc acaatcagca
    21061 caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag gaaaagagta
    21121 cgccacaaac gggaacggcc agtgtgctta agctggcaat actggcaggc aagtttttca
    21181 gcacaaacag ccataagctc cacgctagcg ccgtcgccag aatcgcactg taggccagcg
    21241 cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa gcgaccacac
    21301 tcatcaccag cgccgcatac agcatctgcc aggatgttaa cgataataaa tccacgcgcg
    21361 gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta acaccggaga
    21421 ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc tgcaacacca
    21481 aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc aggcgttcac
    21541 cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc aggatcgcca
    21601 ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc ccgcaggttt
    21661 gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gcgcattccg cgaccacgta
    21721 ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg gtaaagtcga
    21781 aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc cagataagtg
    21841 tgagcacaac caagccactg attgccagtg ggttgctctt tcctgcggca gacatgagag
    21901 atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc agcgactgcc
    21961 tgaaagacag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg aggttcatca
    22021 ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg ctgctggcgt
    22081 aaacttccgc tactgcacgc aggacagagt tagaaccaaa taccagatcc gcacggctgg
    22141 ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct ttcgattcgt
    22201 cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca ttgctcaata
    22261 cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca cccagtacac
    22321 gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc tgtgctttgt
    22381 cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta cggaaaccgt
    22441 cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga cgcgcatcaa
    22501 cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg gctttctcaa
    22561 caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca gactctttct
    22621 ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc cagtcgcgct
    22681 gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca ccacggaagg
    22741 tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca gaatccgcaa
    22801 tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc tgcggcagcg
    22861 gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga gatttcggcc
    22921 ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg aacgcctgcg
    22981 gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc agcgtcaggt
    23041 cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc gggataattt
    23101 ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc tgtacccact
    23161 catacttgaa cagattctcg aagaaatagt tgctccactg gatcggcgtc tgggtccaga
    23221 ctacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa gtgctcgccc
    23281 agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctacg tttgatgtcg
    23341 gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc acggtttctt
    23401 cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca gaaagcggtt
    23461 cgccgctgtg atccgggcct tccgggttaa cgtaaatcag gcccatctcg gttgcaccca
    23521 gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt tcatcacccc
    23581 agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa ccgaaggtac
    23641 ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg gcccaggaga
    23701 ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta tcgaggctta
    23761 cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc gcgccgccgc
    23821 gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc catacgaata aacagaccgg
    23881 cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg gctttcagat
    23941 cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca aagtcctcac
    24001 ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt tgatttggcc
    24061 accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc tgatggaacg
    24121 ggcatttgcc agtggctgtg gtgttatgga tatcgtctga cgtgctcatc aatgtgctcc
    24181 cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat tgaattaatc
    24241 cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag atcaaattga
    24301 tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata aaaaagggtt
    24361 ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct tattttcccg
    24421 ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact gtgtgatttt
    24481 cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca aatcgcgtaa
    24541 ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc acggcttaaa
    24601 atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc ggcatcgtca
    24661 tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt catgtcggca
    24721 aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc ccgggatgat ttccacatca
    24781 atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc gaagaagaac
    24841 tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag caaatccgcc
    24901 tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggatat atcgaaatct
    24961 gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt accacttccc
    25021 ggcggcagat cgccacgcag tgccacgata tgacgaatac cgttattcca gtagtcgcgt
    25081 gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg tgccgcttcc
    25141 agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg ctcgccggag
    25201 ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg atcgatggag
    25261 ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa cgaaacgtta
    25321 atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct ggcgtgaaaa
    25381 aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg cacatctatc
    25441 catttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac cgaaaaatga
    25501 ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt aagaaaaaga
    25561 aaatccgata tgcaaagcaa accggactgc aaatataaac taaccatgta cttattgctt
    25621 cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc tatgataatt caatatattt
    25681 cattagaaac catagacccg aaaacatgag taccgggcat tattaaattt ctgaaattac
    25741 aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac gtcgcgccca
    25801 gcgccaggcc cacggatcac cagcggatta tcgcggtacc agcggctttc gatggcaaag
    25861 acgttatcgc atggcagcag tgatgccagc ggatagtctt cacgcaccgc ctccacgccc
    25921 acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag ccccatttcg
    25981 cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt ttcaaagaaa
    26041 tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg tacctggtcc
    26101 ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca gcttgcgcat tacgtctttg
    26161 ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc ctgatccacc
    26221 agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag cgtgccggag
    26281 aagatcccac tgatcgacaa aatggtatcg ccgctgtcga tcagatcgcg cacggtatgg
    26341 ttgatcggca agcccgcacc aacggtggcg ttgtatagcc agtgacgccc ggttttttcg
    26401 aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg ctcccgccag tttgttggca
    26461 ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact gatcagcaag ctgctggctg
    26521 gcggtaacgt ccaacaccac taaatcatca tacggatggg cgcgcatcca caggaaaagc
    26581 gactcttcat cctgctcgac cgcttcatcg ttgaagaagg ctaacgcgcg gctggcgtcc
    26641 agcccgtcat aactcaacag actgcggcgg ctgtccacca cgcctgccag cacaaattca
    26701 aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga acagttccag ccagcgagag
    26761 ccgatattgc ctttgccgaa caataccagg ccgatgcgtt tttctgcgcg gaagacggac
    26821 tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc caccagactg
    26881 atgccgtcat cggactgcca cgtaaactcg accggctggc ctttcagttg ctgccagaag
    26941 cggtggcaat gcagcgggtt acgggtgaca cccgccccga ccatcgccac cagcgccagc
    27001 ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt cgaggatttt cagcgcactg
    27061 tcggccactt ctgaggtgta gcaaaattgc aacaactggc gatcgttatg cacgccaacc
    27121 gccagtggac gcacctgggc acgtttcagg atctggtcga tctctttatg cgccagtttg
    27181 aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg gctggtgaca
    27241 atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc ttgatccggc
    27301 gtgtagctac agcgcagctg caggtcgatt tcgctgccag aaactggctg taaagtacgg
    27361 gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg cctcatccag acgcagcaac
    27421 ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc ggcgacgtcg
    27481 ctccagatgg ttacacgaga aacacccgcc agcgcaccga tttgtgtcgc ggaatagtcg
    27541 gaaccgttac gccccagcaa caccgtttca ccggcgttgt tacggctgat aaacccggtc
    27601 accaccagac gtttgctcgg atgttgtacc agtagctgtt gcagcaacgg gtaagaaagc
    27661 ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta aaaactcacg ggcatcaagc
    27721 caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgcgc cgaccacact
    27781 tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa taccactgtc gagcaacgcc
    27841 gccaggcgct caagatcgct gacaaaagcg ctaatgaggc tatcggcttc ttcagcgggt
    27901 agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac ctgatgcgca
    27961 gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt ggtgctaccg
    28021 gcggcggaaa ccaccatcat atcgtcaggc tgagagaact ccgccataat gcccgcgaca
    28081 cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag ctgacgacct
    28141 ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc cggaagccat
    28201 tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata cgcagcagcg
    28261 tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt gtcatggttg
    28321 cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta aacaacgaca
    28381 gcccgcccag gaaacgacac agcgtctgct catcgccatc cagttcaaaa ctcaacattg
    28441 cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc ggcagcgacg
    28501 ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc gcctgcgcgt
    28561 tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc agcagatagc
    28621 tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt tcagtgacaa
    28681 cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg ttcagatatt
    28741 tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt aatgccgggc
    28801 ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga tggcagattt
    28861 tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt accagtttgg
    28921 gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca aacaacacgc
    28981 gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg tagcagtcgt
    29041 gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg tgaatcgcgg
    29101 acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc agcgcacgct
    29161 gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc ggttcattaa
    29221 atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa ccatactgtt
    29281 cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc atgtgatgaa
    29341 gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg ttaatggacg
    29401 tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc caaatagccg
    29461 ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg gtaacgcctg
    29521 tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtggggt ctggtctcaa
    29581 tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat atatcagccc
    29641 atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt ccattcctct
    29701 taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca acctgcgtca
    29761 cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg ggcaaccttt
    29821 gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag cggcaaaaga
    29881 gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg gaaaagagcg
    29941 gcgcggagtg gaatcgcctg atgcactacg cttatcaggc ctacgtcata ttgcaattta
    30001 ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg gcataaacaa
    30061 cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta tgaaaaacag
    30121 gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc cgcaaactgg
    30181 cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa gcgatccaac tggcaaccca
    30241 ttccgattac agccacaccg gtatgctggt gatgcgtaac aaaaagccct acgtttttga
    30301 agcggtcggc ccggtgaaat acaccccgct caagcagtgg attgcccatg gtgaaaaggg
    30361 caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc aaaaactgac
    30421 gcaaacggca aaacgttatc tcggtaaacc gtatgatttc agcttctcat ggagcgacga
    30481 tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg gaatgcgcgt
    30541 gggcgaacag cagaagttga aagagtttga tctcagcaac ccgctggtcc aggcgaagct
    30601 caaagaacgc tacggtaaaa atattccgct tgaggaaacg gtagtttcac cacaagccgt
    30661 tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt cgtggtgata
    30721 cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa atccggtctt
    30781 atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca ccggtagcaa
    30841 catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg aggttcaggt
    30901 catgaccaac ggtggagcgg atttttatta cgttaccgca agagcagcta gcagtaattt
    30961 cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag gccgcgtcgc
    31021 tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca atacgatcat
    31081 ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact gatccgcact
    31141 cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat gctatgcccg
    31201 ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg ctgccagaag
    31261 gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag caggagcgca
    31321 tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcactgag ctaaaagcgg
    31381 tagtcgaagt gctggatggt gagccggtgt ttactcactc cgtctggcga ttgctgctat
    31441 gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc ttgccgattt
    31501 tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt gccactgaac
    31561 aaggtcaggc ggtggatctg aacagcctga aacgctcacc caagcaacaa caggcgctgg
    31621 cagcgttacg gcaaggcaaa atctggcgcg accaggttgc cacgctcgaa tttaatgatg
    31681 ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa acaccagagt
    31741 ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg aataccgaac
    31801 aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc tggctgctgg
    31861 cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa aacgtgctcg
    31921 ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg caaactatcg
    31981 cccgttttcg tgaacggttt aatgcgcccg tggaagttct gcattccggt ctgaacgaca
    32041 gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga ggcggcgatt gtgatcggca
    32101 cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt gacgaagagc
    32161 acgacagttc ctacaagcag caggaaggct ggcgctatca tgcccgcgac ctggcggtgt
    32221 atcgtgcgca tagcgagcaa atcccgatta ttcttggctc cgcaacgccc gcgctggaaa
    32281 cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacgcgc cgggcgggga
    32341 atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg tcagaaggtg caggcaggtc
    32401 tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat caggttatcc
    32461 tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg ccacgactgc ggctggattg
    32521 ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag catttgcgct
    32581 gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc ggttccacgc
    32641 acctggtacc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg ccgctgttcc
    32701 ctgatgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg gcgctggaac
    32761 agcaactggc ggaagtacat cgcggcggtg cgaggatttt gatcggcaca caaatgctgg
    32821 cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg gacggcgcgc
    32881 tgttttctgc cgattttcgc tcggcagagc gtttcgctca gctttacacc caggtcgctg
    32941 gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct gcaaacgcac catccggaac
    33001 atcctctgtt gcaaacgttg ctctataaag gctacgatgc ctttgccgaa caggcgctgg
    33061 ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca tgtgattgtg cgtgcggaag
    33121 atcataacaa tcagcatgcg ccgctgttcc tgcaacaact gcgtaatctg atcctctcca
    33181 gcccgctggc agacgacaaa ctgtgggttc tcggtccggt tccggcactg gcacctaaac
    33241 gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc ttcccgcgtg cgcttgcaac
    33301 acatcattag tggtacgctg gcgctcatca atacaatacc ggattcccgc aaggtgaaat
    33361 gggtactgga tgttgatccg attgagggtt aaaccgctta cgatgcgagg cggatcgaaa
    33421 aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg cgctatctgc
    33481 taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg ccaggcgagg
    33541 agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg ttgccctcaa
    33601 ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata aagtctccca
    33661 ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac cgcagcctat
    33721 ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc cggatatctg
    33781 cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa atcacggata
    33841 tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct ttatcgattt
    33901 gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc cgtttgatgc
    33961 cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat ttgcaccgga
    34021 actggagctg cctaccgttc atatcgacaa tctgaccgcc gcatttgatg cagtaaatta
    34081 tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag agatgccact
    34141 gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggaa ttatggttga
    34201 tccacaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag caatgcaaca
    34261 gctgcttgat cttccacaac cgcctactgc agtcttctgc catagcgatg tgatggcgct
    34321 cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc tttccataat
    34381 cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa ccatcgcgca
    34441 gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa tgcaggggca
    34501 acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg gatcaacacg
    34561 cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc cgccgcttaa
    34621 gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca caacgagatt
    34681 atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca cggaaaaagc
    34741 aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc gttcttgtga
    34801 cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc gagacgctgc
    34861 aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc tggcgctaca
    34921 ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct tctgccggtg
    34981 gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt gaacaaatgc
    35041 aggctgatat gcgccagcag ccaacgcagc tggttgaggt tccgtggaat gaacagacgc
    35101 cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta gcggagcagc
    35161 aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag acgcgtacgt
    35221 cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accagcttct actcagcagc
    35281 cgtaccagga tctgctgcaa acccctgcgc acacgaccgc gcagtcgaag ccgcagcaag
    35341 ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa aaagacgaac
    35401 gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag acggtacgtg
    35461 ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc tggaatcgtg
    35521 tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat cggttgaaga
    35581 tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct caaaatcccc
    35641 cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc tgtattcgta
    35701 accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc atgtggtcat
    35761 cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg tgaaaaaggt
    35821 ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg cggatgcttt
    35881 tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc tggttaaagc
    35941 cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac ttgaagcact
    36001 gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg acgtggtgca
    36061 gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg ctgcggcgcg
    36121 cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg cgttggatat
    36181 tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat taagctacaa
    36241 agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg aactggataa
    36301 gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc gtaaccgctg
    36361 gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa atatcctgat
    36421 gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta agctggcgaa
    36481 tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa gtgggctacg tcggtaagga
    36541 agttgattct attattcgcg atctgaccga tgccgccgtg aaaatggtac gcgtccaggc
    36601 tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc tcgacgtgct
    36661 gatcccacct gctaaaaaca actggggaca gactgaacag cagcaggaac cttccgctgc
    36721 tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag aaatcgagat
    36781 cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca tggaagagat
    36841 gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa aagcgcgtaa
    36901 gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga aactggtgaa
    36961 cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga tcgtgtttat
    37021 cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg tttctcgtga
    37081 aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca ccaaacacgg
    37141 gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga ttgcgaaacc
    37201 gtctgacctg atcccggaac tgcaaggtcg gctgccaatc cgcgttgaac tgcaggcact
    37261 gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca ccgtgcagta
    37321 caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg gtattaaacg
    37381 catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg ctcgtcgttt
    37441 acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg atttaagcgg
    37501 tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt tggtggcaga
    37561 tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca ttgtttgatg
    37621 gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa ttagccgaac
    37681 tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg ctgcaattat
    37741 cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg ccctgctggc
    37801 actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt acggcgatgc
    37861 ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc aaaaaggggt
    37921 cattacccag caaaagatga aacgggcgct cattattacc gtcgtgctca tctgtctctc
    37981 cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg gtttcctgat
    38041 tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc gtccttatgg
    38101 ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga gtgtcatggg
    38161 gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg cgaccgcatg
    38221 cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca atagcgaccg
    38281 cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc gtcgttatca
    38341 tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct tttcgctgca
    38401 tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc aagcccgtta
    38461 tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta ctgtcaaggg
    38521 agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt gggcagcata
    38581 actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag cgcgatatac
    38641 tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc cgagctttgt
    38701 gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt tggcggacgg
    38761 gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg gttgctgtac
    38821 gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg tggttctgtt
    38881 cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga atgggaaggt
    38941 ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga tatcggcatc
    39001 caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga aagcgatgtc
    39061 cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc cgacaatacc
    39121 gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca ccgcaaggtg
    39181 ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgaa agatgaagcg
    39241 taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc agccgttctg
    39301 ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct gggcattttg
    39361 aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca tctgcaacag
    39421 agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca cttctaatga
    39481 cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct cgtcccgatt
    39541 accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt atgaagccgc
    39601 gccatcgctg tccagcggca cgccttgcag attacggttt gccacacttt tcatccttct
    39661 cctagtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc gaatgatcta
    39721 acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct cattttatga
    39781 cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg ataagttaac
    39841 agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa acattaactc
    39901 ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt gctgaattcc
    39961 tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta aaagtcgctg
    40021 gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg gcaatggcca
    40081 tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc attgcattgt
    40141 ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca caagttgccg
    40201 gcgctttctg cgctgcggct ttagtttacg ggctttacta caatttattt ttcgacttcg
    40261 agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct ggcactttct
    40321 ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag atggtgatta
    40381 ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt gtaccacgcg
    40441 gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca tctatgggcc
    40501 cattgacagg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc tttgcctagc
    40561 tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac ttcctggtgc
    40621 cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc aaactgattg
    40681 gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca actccttcag
    40741 aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga aaaaaatata
    40801 tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat cacgatgcca
    40861 atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca ggttgggtag
    40921 aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa gtgctggcga
    40981 aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag cgtgaaacca
    41041 ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc tggcagtgcc
    41101 gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat tatatccgca
    41161 gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag tggatcctcg
    41221 accatgtgga aggctctcac gagcgtgcac gtcgtggtga attgctgttt ggtacggttg
    41281 atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat tacaccaacg
    41341 cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa atgctggaag
    41401 tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa gtatacggtc
    41461 agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc gccggtgacc
    41521 agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag aacacctatg
    41581 gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca gaaaacggcc
    41641 tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg gaaggtgcgg
    41701 tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg attaacgacg
    41761 cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg tatgtggttc
    41821 cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg gcgattttcg
    41881 gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag tctattgctt
    41941 atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt ctgcacgccc
    42001 tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag tccgatattc
    42061 tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt gcggcctatc
    42121 tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag aaagcggtga
    42181 ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt tacgcaggct
    42241 ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa tgtcaggcac
    42301 ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct tacgcgtctt atcaggccta
    42361 cgagccaatg ccattttgtg taggcgggat aaggcgttca cgccgcatcc ggcatcacgc
    42421 agactctgaa accgtgcggg ggcaaccccg cacacatcaa taatcccacc cttcccctgt
    42481 gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga acttgccatc
    42541 gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggtt aggacgcggc
    42601 gataaaaaca ccgcggacgg cgcggcggta aacgccatgc gtattatgct caaccaggtc
    42661 aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc gatgctctac
    42721 attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt tgatccgatt
    42781 gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct ggcagtaggc
    42841 gataaaggct gcttcctcaa tgcgccggat atgtatatgg agaagctgat tgtcgggccg
    42901 ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg caatgtagcg
    42961 gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga ttctggctaa accacgccac
    43021 gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat tccggacggc
    43081 gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt gctgtacggt
    43141 attggtggcg cgccggaagg cgtggtttct gcggcggtga tccgcgcatt agatggcgac
    43201 atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga gaatcgtcgc
    43261 attggcgagc aggagctggc acgctgcaaa gcgatgggca tcgaagccgg taaagtattg
    43321 cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg tattaccaaa
    43381 ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg cgactaccga aacgctgctg
    43441 atccgcggca agtcacgcac cattcgccgc attcagtcca tccactatct ggatcgcaaa
    43501 gacccggaaa tgcaggtgca cattctctga gtgatttgat cgattgagcc ttccagtcct
    43561 tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc aaaacaggag
    43621 aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg gaccgacgcc
    43681 ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca atttaccaag
    43741 cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt taactcgccc
    43801 gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt aagcccacga
    43861 ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc tggcttcttt
    43921 gttctggatg aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg tacagcgatt
    43981 ggcccttatt tatcgattct gcaactaggc aaagatttag atcgcttcaa aaatctggtc
    44041 ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat gcaagaactg
    44101 gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga aacggcagcg
    44161 gggtcgctca ccggacggat accggcatta attgaaagtg gggaactgga aagcgcgatt
    44221 ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc acagatggtg
    44281 cgcgatacac aacagttgct gaaagagacc cggcagatga cgaaacattt acgtcgccga
    44341 ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata aacggcacga
    44401 tgagcaaatc cgcactcatc ttattgatca tcccgcgata tgccggcatc aaacggttga
    44461 taaatgagtg atgatgacca cagacaagga ggtcgcactg ctctttttgc ataatttcca
    44521 gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc cattgaatat
    44581 ttttcgtcag tttatacagc ttgttatccg acttattctt caacaattga agaatatctt
    44641 ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca atatgaatta
    44701 acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttattcacc agtaaggcat
    44761 cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt aactccttct
    44821 aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca tctcggcctg
    44881 gcatgagaaa gtgaaggccg catcaagctc aacttgcccc tccagatcct taatcttata
    44941 ggccacgctg gccccttcat ctgcagagga tgcagcggcg gcaagctctt tcagtttggt
    45001 cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac tggtacagtt
    45061 gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc atttatctac
    45121 gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga cttaagaata
    45181 atgcgccgtt aagggaagaa caaacaagta atgatcttat ggattaccat tttttttgat
    45241 attgcttaag gtgaagagtt cagaaggatt ttccgggtat tgtcatttgc atcgtggaaa
    45301 taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggttaatta cttgatttat
    45361 tgtcggcttt atacttcaca tcctgagtat ctttaccata tttattttcg ccttgtgtgc
    45421 caacaaacgc accaagatcg ataagcatca tcaccagaat caacgtcggg acaaaacgcc
    45481 ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt acccgccagc agcatccacg
    45541 ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag cgcttaacag
    45601 ttactgccgc tgttggccag agcaagcaca caaggcaaaa cgccgcggtc tgaatatcga
    45661 gtagattctt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac aggcctatcc
    45721 aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat tgctgtatgg
    45781 tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac aagggcgaca
    45841 gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag ccagggtgta
    45901 cgctgttttt tctcttatgt tctgcattaa ccgttacaac aacggcgcat gcacaaacac
    45961 cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc gatctctcca
    46021 tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg aacgaatttc
    46081 gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgcaagt aaaattaacg
    46141 agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa agcattcaaa
    46201 tgacctggct acccatccag gggccagagc aaaaagcagc gaaagcgaaa gctcaggaat
    46261 acatggcagc agtgatccgc acactcaccc cattaatgac caaaacacaa agccagaaaa
    46321 aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag acagaaggtg
    46381 cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct gttgaaccga
    46441 ttaagctggc gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa gcaaagcctt
    46501 tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc ggccatcttc
    46561 ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga tgggtaactg
    46621 gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc gtaaagagct
    46681 ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca tcgatatggc
    46741 gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg acctgaacct
    46801 gtccggcgca ttcaccggtg aaacttctgc cgctatgctg aaagacatcg gcgctcagta
    46861 catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg aactgattgc
    46921 gaaaaaattc gcggtgctga aagagcaggg tctgactccg gttctgtgca tcggtgaaac
    46981 cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga tcgacgcggt
    47041 actgaaaact cagggtgctg cggcattcga aggtgcggtt atcgcttacg aacctgtatg
    47101 ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc acaaattcat
    47161 ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca ttcagtacgg
    47221 cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca tcgacggcgc
    47281 gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta aagctgcaga
    47341 agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca cttactttcc
    47401 ttaactcctc gccttaacgc aaaatctcac actcatgatc ctggatttcc tcggctgaag
    47461 cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa ttatcactct
    47521 gccgtaccat cgccagcccg tagcttccca tatgttcccg cgcctcaggc acttcttctg
    47581 caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga cgcgccaggt
    47641 attcatgccc gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg ttcgccagat
    47701 ttttatccag ttgttcgcgc acatcaggac gaatacaaga gatatgaata tgaaaatggt
    47761 tttgcgtatg cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg ggaaccggct
    47821 ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag aagttcggcg
    47881 ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt ggcatcaaca
    47941 gatattgcag tggaccatta agatctttta aaaccacgta tccggcattg ggtttgactt
    48001 ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc tcaaggacaa
    48061 tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata ccggcagcca
    48121 caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc tttttttccc
    48181 tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa cccggtcgcg
    48241 agaaaccacc gggtttgata attatcctag ggaaatcagc gtttgctgat ctgatcgaac
    48301 gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa ctcttcatca
    48361 atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgctac ttcagcgtcg
    48421 cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta gagatatttc
    48481 aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc gaccaccgac
    48541 accgttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt atctttcccc
    48601 agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc gccaatcccg
    48661 cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat ccagaacttc gacgttttta
    48721 tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg atgcagcgcg
    48781 tagccccagg cagccaggta gttccagcgt gcgccaccag agcttttcgg attaggcgtg
    48841 atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt cggattgccc
    48901 ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg cagacgtttg
    48961 atccactctt tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata ggccagagcc
    49021 agcgtgacaa catcagcttc aataccgttg attaccgacg tcgcttgttt acctgaacca
    49081 ccgtgtgact gacgaatcac cacgttatca ccagtttgct gtttccagtg ggcgctgaat
    49141 gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt aagaagctga
    49201 atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac gccccacttg
    49261 ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag aaggttaacc
    49321 aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg agggattaaa
    49381 aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat gccggatgcg
    49441 gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc agaattcatg
    49501 taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt tcaggctaaa
    49561 ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca gccagtcacc
    49621 tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa ccagctgttc
    49681 gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga tagcgtaagc
    49741 gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc gctggatgtg
    49801 gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat gcgccagttc
    49861 gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac ctttcgcgat
    49921 acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg gaactacaac
    49981 gaattcacag ccaccggcaa tggccgcagc cagggtcaga tcgccacaat aacggcccat
    50041 cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac ggtcgatcgc
    50101 ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc ctttgatgtc
    50161 gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca gacgcattgc
    50221 acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac gttttttcag
    50281 gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac gcgcagaacc
    50341 gaggaacgta ccgccacggt tgatcatgtc agacacgctg taacggtcta gctgtaccat
    50401 acggtcttca tacagaccca gatagccgtc ataaataccc attacttcca gaccttctgt
    50461 cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat caccgccgct
    50521 tgtcaacaca cctattttct taatcatgac tacctctgaa ctttggaatg caaaatgaaa
    50581 tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca aatagtataa
    50641 caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt atacacaaaa
    50701 tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac cctccctggg
    50761 tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac gccgtaaaat
    50821 agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag agtcttccat
    50881 ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa gatcgtgagc
    50941 gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt cttcatcagg
    51001 caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg cgctatataa
    51061 aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc cgtaccaaga
    51121 caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt aatgtagcat
    51181 atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa acgagacaag
    51241 gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg gatctgtcat
    51301 cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg ccgaaccgga
    51361 gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac caaacgagtg
    51421 attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca acgacgcgcc
    51481 gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg tataccacca
    51541 tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa tcgccgcccg
    51601 actgaccagc cgtccataag attgattcat aaatactccc gctatcaact gacgctagta
    51661 taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga taaaaaaaac
    51721 cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca gggtttacta
    51781 ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt gccattgcgt
    51841 cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg cttgctgctc
    51901 cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct gacgagcaat
    51961 ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa aattttctgc
    52021 ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct gttcgtgccg
    52081 ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac ttatgccgtc
    52141 gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc agttatcgcc
    52201 tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg ccatgacggc
    52261 agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc aacgatagag
    52321 agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta aagtcatgga
    52381 ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg aataaaatcc
    52441 tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc gagatggaag
    52501 gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg gacgacagca
    52561 ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac acattaaaag
    52621 cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc agtgaacttg
    52681 atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg tttaatgatc
    52741 gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc gcactggagc gagcaacagc
    52801 aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg aatccaggcc
    52861 gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag tttaccctgc
    52921 tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta agccaggaag
    52981 tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt tccaacctgc
    53041 gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg cgtggtcgcg
    53101 gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct tcgccatctt
    53161 ctggctgacg ctggtgctgg tgttgatgtt ggttttgatg ttacccaagc tcgattcacg
    53221 ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg agcagcatgt
    53281 cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc gtctgttccg
    53341 ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca ccgaaggccg
    53401 cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg gtcaggccga
    53461 taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg gtccgttctc
    53521 cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca gtagttctca
    53581 atccgatttc attaacttat tgtttgaccg cccgctatta ctgctgattg tcaccatgtt
    53641 ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg cgcgtaagct
    53701 gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg aactggaagc
    53761 ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca ccgcgctgga
    53821 gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc tgcgcacccc
    53881 gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg aaagcaagga
    53941 actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg atctgttggt
    54001 gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag ccaaccagtt
    54061 gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca agtcgttgac
    54121 agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc tggaaagtgc
    54181 gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg aagtgggctt
    54241 tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg gcgttagccc
    54301 ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac gcgatcgtga
    54361 atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc agcatcgtgg
    54421 ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt ggttgccgct
    54481 gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg ccgcatccgg
    54541 catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta cactccgggg
    54601 gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt cggcgctttt
    54661 gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg tgatactgat
    54721 cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc acatcgctga
    54781 cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct atcacgctat
    54841 acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg atatcaaaat
    54901 ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat gcctctcccc
    54961 agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt gagagatttt
    55021 cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc acccagtaga
    55081 gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga acctttttct
    55141 ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca tcaacctgga
    55201 ttttggcaat tgcgctgggt ttgccttcgg gataagcctg aatcttgcct gtgtatacat
    55261 caaccggata tcgcatctgt tccctctcca cgagaaattt ccccattaga ccatatttat
    55321 tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc aggaactca
//
LOCUS       DAJTQQ010000017        55079 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000017 DAJTQQ010000000
VERSION     DAJTQQ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.017"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..313
                     /locus_tag="OO309_001121"
     CDS             <1..313
                     /locus_tag="OO309_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR5846334.1"
                     /translation="QGELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQIGF
                     QLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMY
                     N"
     gene            complement(351..1250)
                     /gene="nhaR"
                     /locus_tag="OO309_001122"
     CDS             complement(351..1250)
                     /gene="nhaR"
                     /locus_tag="OO309_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR5846335.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1316..2482)
                     /gene="nhaA"
                     /locus_tag="OO309_001123"
     CDS             complement(1316..2482)
                     /gene="nhaA"
                     /locus_tag="OO309_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR5846336.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3068..3220
                     /gene="mokC"
                     /locus_tag="OO309_001124"
     CDS             3068..3220
                     /gene="mokC"
                     /locus_tag="OO309_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR5846337.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3324..4454)
                     /gene="dnaJ"
                     /locus_tag="OO309_001125"
     CDS             complement(3324..4454)
                     /gene="dnaJ"
                     /locus_tag="OO309_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR5846338.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4543..6459)
                     /gene="dnaK"
                     /locus_tag="OO309_001126"
     CDS             complement(4543..6459)
                     /gene="dnaK"
                     /locus_tag="OO309_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR5846339.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6836..7240
                     /locus_tag="OO309_001127"
     CDS             6836..7240
                     /locus_tag="OO309_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR5846340.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7266..7979
                     /gene="msyB"
                     /locus_tag="OO309_001128"
     CDS             7266..7979
                     /gene="msyB"
                     /locus_tag="OO309_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR5846341.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8128..8694
                     /gene="satP"
                     /locus_tag="OO309_001129"
     CDS             8128..8694
                     /gene="satP"
                     /locus_tag="OO309_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR5846342.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8729..9316)
                     /gene="mog"
                     /locus_tag="OO309_001130"
     CDS             complement(8729..9316)
                     /gene="mog"
                     /locus_tag="OO309_001130"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR5846343.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9431..10384)
                     /gene="tal"
                     /locus_tag="OO309_001131"
     CDS             complement(9431..10384)
                     /gene="tal"
                     /locus_tag="OO309_001131"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5846344.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10663..12093
                     /locus_tag="OO309_001132"
                     /pseudo
     CDS             10663..12093
                     /locus_tag="OO309_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            12163..12939
                     /gene="yaaA"
                     /locus_tag="OO309_001133"
     CDS             12163..12939
                     /gene="yaaA"
                     /locus_tag="OO309_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR5846345.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13092..13388)
                     /locus_tag="OO309_001134"
     CDS             complement(13092..13388)
                     /locus_tag="OO309_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5846346.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13602..14888)
                     /gene="thrC"
                     /locus_tag="OO309_001135"
     CDS             complement(13602..14888)
                     /gene="thrC"
                     /locus_tag="OO309_001135"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR5846347.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14889..15821)
                     /gene="thrB"
                     /locus_tag="OO309_001136"
     CDS             complement(14889..15821)
                     /gene="thrB"
                     /locus_tag="OO309_001136"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR5846348.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15823..18285)
                     /gene="thrA"
                     /locus_tag="OO309_001137"
     CDS             complement(15823..18285)
                     /gene="thrA"
                     /locus_tag="OO309_001137"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR5846349.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18311..18430)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18366..18431)
                     /gene="thrL"
                     /locus_tag="OO309_001138"
     CDS             complement(18366..18431)
                     /gene="thrL"
                     /locus_tag="OO309_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR5846350.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18645..19331)
                     /locus_tag="OO309_001139"
     CDS             complement(18645..19331)
                     /locus_tag="OO309_001139"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5846351.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19731..19871)
                     /locus_tag="OO309_001140"
     CDS             complement(19731..19871)
                     /locus_tag="OO309_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846352.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            19967..20683
                     /gene="arcA"
                     /locus_tag="OO309_001141"
     CDS             19967..20683
                     /gene="arcA"
                     /locus_tag="OO309_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR5846353.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20742..22094)
                     /gene="creD"
                     /locus_tag="OO309_001142"
     CDS             complement(20742..22094)
                     /gene="creD"
                     /locus_tag="OO309_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR5846354.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22152..23576)
                     /gene="creC"
                     /locus_tag="OO309_001143"
     CDS             complement(22152..23576)
                     /gene="creC"
                     /locus_tag="OO309_001143"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR5846355.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23576..24265)
                     /gene="creB"
                     /locus_tag="OO309_001144"
     CDS             complement(23576..24265)
                     /gene="creB"
                     /locus_tag="OO309_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR5846356.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALYPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24278..24751)
                     /gene="creA"
                     /locus_tag="OO309_001145"
     CDS             complement(24278..24751)
                     /gene="creA"
                     /locus_tag="OO309_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR5846357.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            24962..25831
                     /gene="robA"
                     /locus_tag="OO309_001146"
     CDS             24962..25831
                     /gene="robA"
                     /locus_tag="OO309_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR5846358.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25828..26475)
                     /gene="gpmB"
                     /locus_tag="OO309_001147"
     CDS             complement(25828..26475)
                     /gene="gpmB"
                     /locus_tag="OO309_001147"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR5846359.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26527..27047
                     /gene="yjjX"
                     /locus_tag="OO309_001148"
                     /pseudo
     CDS             26527..27047
                     /gene="yjjX"
                     /locus_tag="OO309_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(27132..27458)
                     /gene="trpR"
                     /locus_tag="OO309_001149"
     CDS             complement(27132..27458)
                     /gene="trpR"
                     /locus_tag="OO309_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR5846360.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27548..29485)
                     /gene="sltY"
                     /locus_tag="OO309_001150"
     CDS             complement(27548..29485)
                     /gene="sltY"
                     /locus_tag="OO309_001150"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR5846361.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29696..31363
                     /gene="ettA"
                     /locus_tag="OO309_001151"
     CDS             29696..31363
                     /gene="ettA"
                     /locus_tag="OO309_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR5846362.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31470..31637)
                     /locus_tag="OO309_001152"
     CDS             complement(31470..31637)
                     /locus_tag="OO309_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846363.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31671..32903)
                     /gene="nadR"
                     /locus_tag="OO309_001153"
     CDS             complement(31671..32903)
                     /gene="nadR"
                     /locus_tag="OO309_001153"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR5846364.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32924..34306)
                     /gene="radA"
                     /locus_tag="OO309_001154"
     CDS             complement(32924..34306)
                     /gene="radA"
                     /locus_tag="OO309_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR5846365.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34355..35323)
                     /gene="serB"
                     /locus_tag="OO309_001155"
     CDS             complement(34355..35323)
                     /gene="serB"
                     /locus_tag="OO309_001155"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR5846366.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35429..36073
                     /locus_tag="OO309_001156"
     CDS             35429..36073
                     /locus_tag="OO309_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR5846367.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36101..37116
                     /gene="lplA"
                     /locus_tag="OO309_001157"
                     /pseudo
     CDS             36101..37116
                     /gene="lplA"
                     /locus_tag="OO309_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37148..37411
                     /locus_tag="OO309_001158"
     CDS             37148..37411
                     /locus_tag="OO309_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5846368.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37572..38291)
                     /gene="deoD"
                     /locus_tag="OO309_001159"
     CDS             complement(37572..38291)
                     /gene="deoD"
                     /locus_tag="OO309_001159"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR5846369.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38348..39571)
                     /gene="deoB"
                     /locus_tag="OO309_001160"
     CDS             complement(38348..39571)
                     /gene="deoB"
                     /locus_tag="OO309_001160"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5846370.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39623..40945)
                     /gene="deoA"
                     /locus_tag="OO309_001161"
     CDS             complement(39623..40945)
                     /gene="deoA"
                     /locus_tag="OO309_001161"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR5846371.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41072..41851)
                     /gene="deoC"
                     /locus_tag="OO309_001162"
     CDS             complement(41072..41851)
                     /gene="deoC"
                     /locus_tag="OO309_001162"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR5846372.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42109..43650
                     /locus_tag="OO309_001163"
     CDS             42109..43650
                     /locus_tag="OO309_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR5846373.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43622..44485
                     /locus_tag="OO309_001164"
     CDS             43622..44485
                     /locus_tag="OO309_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR5846374.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44699..45481)
                     /locus_tag="OO309_001165"
     CDS             complement(44699..45481)
                     /locus_tag="OO309_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5846375.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45478..46551)
                     /locus_tag="OO309_001166"
     CDS             complement(45478..46551)
                     /locus_tag="OO309_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR5846376.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46673..46834)
                     /locus_tag="OO309_001167"
     CDS             complement(46673..46834)
                     /locus_tag="OO309_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR5846377.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(46951..47556)
                     /gene="osmY"
                     /locus_tag="OO309_001168"
     CDS             complement(46951..47556)
                     /gene="osmY"
                     /locus_tag="OO309_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR5846378.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(47949..49538)
                     /gene="prfC"
                     /locus_tag="OO309_001169"
     CDS             complement(47949..49538)
                     /gene="prfC"
                     /locus_tag="OO309_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR5846379.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49629..50306)
                     /gene="yjjG"
                     /locus_tag="OO309_001170"
     CDS             complement(49629..50306)
                     /gene="yjjG"
                     /locus_tag="OO309_001170"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR5846380.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50321..50767)
                     /gene="rimI"
                     /locus_tag="OO309_001171"
     CDS             complement(50321..50767)
                     /gene="rimI"
                     /locus_tag="OO309_001171"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5846381.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50736..51149)
                     /gene="holD"
                     /locus_tag="OO309_001172"
     CDS             complement(50736..51149)
                     /gene="holD"
                     /locus_tag="OO309_001172"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR5846382.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51252..52283
                     /gene="rsmC"
                     /locus_tag="OO309_001173"
     CDS             51252..52283
                     /gene="rsmC"
                     /locus_tag="OO309_001173"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR5846383.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            52560..52646
                     /locus_tag="OO309_001174"
     tRNA            52560..52646
                     /locus_tag="OO309_001174"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52594..52596,aa:Leu,seq:cag)
     gene            complement(52685..52921)
                     /locus_tag="OO309_001175"
     CDS             complement(52685..52921)
                     /locus_tag="OO309_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR5846384.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            53062..53850
                     /gene="fhuF"
                     /locus_tag="OO309_001176"
     CDS             53062..53850
                     /gene="fhuF"
                     /locus_tag="OO309_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR5846385.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            complement(53888..54565)
                     /gene="bglJ"
                     /locus_tag="OO309_001177"
     CDS             complement(53888..54565)
                     /gene="bglJ"
                     /locus_tag="OO309_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR5846386.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(54523..>55079)
                     /locus_tag="OO309_001178"
     CDS             complement(54523..>55079)
                     /locus_tag="OO309_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5846387.1"
                     /translation="DLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELL
                     MCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSER
                     KVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGAREL
                     NLPSVYGDAAEWNTAELRREMSHS"
BASE COUNT        13179 a        14875 c        13734 g        13291 t
ORIGIN      
        1 gcaaggtgaa ttagtttttg aacccagtga cgttgaatat tactttgaac cagtgacgat
       61 acaggagtca ggaaccagca tattaaaaaa tgagttagaa aacagtgagg acggtgcggg
      121 tcaaataggc tttcaactct ccaatgatgg tacgaatgaa atccagtatg gtaaaagcaa
      181 ttactactct tttcagcatc cccatgaagg aagtaatcag attcctctat ttattcgccc
      241 gcgtacatat ggaaacaatg tctcaagcgg gcaaataatg agccgagtca aaatcgtggt
      301 aatgtacaac taatctatct cccccacctt aactttgggg gagctgccga ttaacgcacc
      361 gctggactaa aaagcgcaga ataatccgta ttgcagattc gctgtaccgc cgggtgctga
      421 atcatccgct cagcaaaaat agcatggtac tcttccatca cattctcgac gcgaccaatt
      481 tctacgacag ttttatcggc ataaaagtca tatgcataaa gcgttggggc aacgaagatt
      541 gcattgtgca tcgcaccaaa agctttcatc aaagcggcat catcaaactc gccgaggatt
      601 tctacgttta atccctggga gttaaaccag ttaagcaatt tgcgccctaa cattgaacgt
      661 cgcccaggaa tcaaaagtcg ccgttcttcc agacaagccg ggaacggttt ttctggtggt
      721 ggatttgtac accagaaact cacgccacat tcgccaattc tcacggagaa caggccttcc
      781 tgctgcgtag agtctatcgg acagtcagaa atgatcatat ccagtttatg ctgacttaat
      841 tgctccagca gcatttcgtg ggtggattcg aagcagcgaa gatgaatggg ctcgccttct
      901 accactgcgg cgttaagtac gctactgacc aggcgtttgg aaagtgcatc agccacgcca
      961 acgtcaaaca ataaattgga ttctttgcga tagttcacaa tatccagcat ttcctggctt
     1021 aaggtgaaca ttttatcggc atagcgatag accagttctc ccagctcgct gggttcgaga
     1081 ccacgtccct tgcgtttaaa taatttgcct tgcaggcgct cttccagcgc tcgaatctgt
     1141 ccggtaatgg tttgtggagt taaataaagc gcctccgctg cgccaaccac ggaaccttct
     1201 ttatagacat gccagaagta atacaagtgg ttgtaattga tatgagacat gctcatttct
     1261 ctccctgata acaatgaaaa gggagccgtt tatggctccc cggtaaaccg tcctgtcaaa
     1321 ctgatggacg caaacgaacg cgtaaccagc tgtatccaat taccgccgaa gagatagaac
     1381 cgaccaggat accgagtttc gcccagttaa tcagttctgg atctacgcta ccaaaggcca
     1441 ggctggcaat aaagatagac atagtaaaac cgataccgca caggatcccc accaccataa
     1501 tttgctgata agtcgttccc tcaggcagat gcgccagttt caaacgcagc gccaaccagc
     1561 agaacagact aatccccagc ggtttgccaa tcagcaagcc agcgatgatc cccaatggca
     1621 gaatggaggt caagccatcc agcgtgacgc cttgcagtga aacgccagca ttagcaaatg
     1681 caaacagcgg caaaatcaga tacgccaccc acgggtgcaa cacatgctcc agtcgcttcg
     1741 ctggagaacg cccatgcttc tctttcaaag gaataaagaa gccgacaatt acccccgcca
     1801 gagttgcgtg aacccccgat ttcaacaccg cagtccacaa caccacgcca acaagaatat
     1861 agacgcccgt gcggcgtaca ccacacagat tcaataccgc gagtaccgca attgctacag
     1921 ccgcgacgcc aagggaggcc atcgataagt cattagtgta gaacaatgcg atgataatga
     1981 tggccccaag atcgtcgata atagccagag ccatcaaaaa gatcttcagc actaacggaa
     2041 cacgacttcc caacagcgcc agtacaccaa gtgcaaaagc aatgtcagta gccgccggga
     2101 tcgcccaccc ttcgcgggta atcggatcgg catagttaaa agccagatag agtaatgccg
     2161 gcacaatcat cccaccaata gcggcgataa ctggaaatgc ggcctggcgt aagctggcta
     2221 gcgatccttg catcagttca cgtttaactt ccagaccgac taacaggaaa aataccgcca
     2281 tcagcgcatc atttatccat aacagcatgt ttttgttgat ttcgagtgaa ccaacccgga
     2341 gctgaaccgg cgtctccaga aagtcgtgat accatccact ggttgcgccg ctgttggcca
     2401 taatcatcgc caggatagcg gcaatgataa gaataatgcc tcccgaggca tcactgctaa
     2461 agaatcgatg cagatgtttc actttttatt tctctttcag gtgaatagat cgtttttact
     2521 attttacccc gcacgaatca tcgttaaaat cagattatag tgtaggaata attcgttttt
     2581 tacgagccat tgaagaattt gcgccatcag cggtgagatg cgtttttgac ggctgtaagc
     2641 gctcacgagt gaccctaagc ccagataatc atagcttaag gtgaggattc ctgagggatt
     2701 aattaggaaa agtaataatt aatttgttgt cagtcggcga tgcttgttag gccaatattt
     2761 caaaaataat ccagcttgtc gtcaataatt gcgatgaaga gataaattaa agttaaataa
     2821 ttaaatcatt aaaaaattac gccacaaaaa tgcgaggcgt cttcaggatt agaatcgata
     2881 aaacaaaatg ggttaaagag agcgatataa tagcggcggg tgcttgaggc tgtctgtctc
     2941 aggcattagc tgaacggcag atagagaaaa gccccgagtg atattttacc atcaacccga
     3001 ggcctcctat atgctgaaca catgtagagt gcctcttact gaccgtaagg tcaaggagaa
     3061 gagagcaatg aagcagcata aggcgatgat tgtcgccctg atcgtcatct gtatcaccgc
     3121 cgtagtggcg gcgctggtaa cgagaaaaga cctctgtgag gttcacatcc gaactggcca
     3181 gacggaggtt gctgttttca cggcttacga atccgagtaa gagcaacggc ggggagtgat
     3241 ccccgccact ttacaggtgc tcgcatatct tcaacgcacc ctatttttac ccaggcctgc
     3301 ccacgggcag gcttttgggg aggttagcgg gtcaggtcgt caaaaaactt cttcacacca
     3361 tcaaagaagc tctttgagcg cgggctgttg tgctcgccgg ttgggccacc gaagctttct
     3421 tgcagctctt gcagcagctg tttctgcttt tcgttcaggc ctaccggtgt ttcgacgaca
     3481 acgcggcaca gcaaatcacc ctgtgcgcca ccgcggacag acttgacgcc tttaccgcgc
     3541 atacggaata gcttaccggt ctgggtttcg ccaggcactt tcagtttgac gcgaccatca
     3601 agggtcggta cttcgatttc gccacccagc gccgccatag cgaagttgat cgggacttcg
     3661 caatacaggt tgttgccttc acgctcgaaa atcgggtgct gtttaacctg aacctgaacg
     3721 tacagatcgc ctgccggtgc gccatgctcg cccgcttcac cttcgcccgc aagacggatg
     3781 cggtctccag tgtccacccc tgccgggatt ttaacggaca gcgttttgct gcgctcaaca
     3841 cgaccatgac catgacattt gttgcacgga tctttgatca gcgtaccgcg gccctgacag
     3901 tgtggacagg tctgctgtac agcgaagaat ccctggcgca tctgcacctg accagaacca
     3961 tgacaggtcg gacaggtctg cggctgtgta cctggttttg caccgctacc gtggcaaacg
     4021 tcacactctt ccagagtcgg aatgcggatc tctttggtca cgccacgtac agcttcttcg
     4081 agggtgagct ccatgttata gcgtaaatca gcaccgcgcg ccgcacgttg acgaccacgt
     4141 ccgccgccaa aaatatcgcc gaaaacgtca ccaaaaatat cgctgaagtc tgcgccgccg
     4201 ccaaaaccac cgccgcccat gcctccttgc tcaaacgcag catgaccata ctgatcgtat
     4261 gccgcacgtt tttgcgagtc ggtcagaact tcataagctt ccttgatctc tttaaatttc
     4321 gcctcggcct ctttgtcacc ctggttacgg tccgggtggt atttcatggc caggcgtttg
     4381 taggcctttc tgatttcacg ctcttccgct gttttggaaa cgcctaaaat ctcgtaataa
     4441 tcttgcttag ccatcttttt taaattgccc ctagatgaat gcacgggcgg agaggaaatt
     4501 ccccttcgcc cgtgtcagta taattacccg tttatagggc gattattttt tgtctttgac
     4561 ttcttcaaat tcagcgtcga caacatcgtc atctttcgcg ttgtttgcag aagcatcagc
     4621 accggcagtc tgctgctggg catgttgctg ctgggcgatt tccatcagtt tctgggaaac
     4681 ctgtgccagt tcctgcattt tcgcttcgat agcggctttg tcttcacctt tcagagcagt
     4741 ttccagtgca gtcagcgcag actcgatagc agttttgtcg tcagccggca gtttgtcgcc
     4801 tgcttcttca acctgcttac gggtgctgtg cagcagatgg tcgccctggt tgcgagtctg
     4861 taccagctct tcaaacttac ggtcagcttc ggcgttagct tctgcgtcgc gtaccatttt
     4921 ctggatttca tcttcgttca gaccagaaga agccttgatg gtgatcttct gctctttacc
     4981 gctgttttta tctttcgcgg aaacgtgcag gataccgtca gcatcgatat cgaaggtaac
     5041 ttcgatctgc ggcatgccgc gcggtgccgg gttgatacca tctaggttga actgacccag
     5101 agatttgtta tcagccgcac gtttacgttc accctgcagc acatggatgg ttaccgcaga
     5161 ctggttgtct tcagcggtag agaacacctg gctgtgcttg gtcgggatag tggtgttttt
     5221 cgcgatcagc gtcgtcatca caccgcccat ggtttcgata cccagagaca gcggggtaac
     5281 gtccagcagc agtacgtctt ttacgtcacc agtcagaaca ccaccctgaa cagcagcacc
     5341 gattgctaca gcttcgtccg ggttaacgtc tttacgcggc tctttaccaa agaactcagc
     5401 aactttcttc tgaaccattg gcatacgagt ctgaccacca acgaggataa cgtcgtcgat
     5461 atcagatacg gacaggccag catcctgcag tgcaactttc agcggctcaa tggaacggtt
     5521 taccagatct tcaaccaggc tttccagttt cgcacgagtc actttgatgt tcatgtgttt
     5581 cggaccggtc gcgtctgcag tgatgtatgg caggttaacg tcggtctgct gagcggaaga
     5641 cagttcgatt ttcgcttttt ctgccgcttc tttcaggcgc tgcattgcca gcggatcgtt
     5701 gcgcaggtca atgccctgat ctttcttgaa ttcttcaacc agatagttga tcagacggct
     5761 gtcgaagtct tcaccaccca ggtgggtatc accgttggtt gccagaactt cgaaggtttt
     5821 ttcgccgtca acttcgtcga tttcgataat agaaatatcg aaagtaccac cccccaggtc
     5881 ataaaccgcg atagtacggt tgccagtgcc tttgtccaga ccgtaagcca gcgcagctgc
     5941 ggtcggttcg ttgatgatac gttttacttc cagaccagcg atacggcctg cgtctttggt
     6001 tgcctgacgc tgagcatcgt taaagtatgc cggtacggtg ataacagctt cagttaccgg
     6061 ttcacccagg taatcttcag cggttttctt catttttttc agcacttcag cagaaatctg
     6121 cggcggtgcc attttctggc ctttaacttc gacccatgcg tcgccgttat cagcagcaat
     6181 aattttgaac ggcatgatgg aaacatcacg ctgtacttct tcgtcctgga agcggcggcc
     6241 aatcaggcgt ttaatcgcaa acagggtgtt ttgcgggttc gtcactgcct gacgtttagc
     6301 cggctgacca accagagttt caccatcctg ggtataggca atgatagaag gcgtggtgcg
     6361 atcgccttcg gcgttctcca gcacgcgagg aatggtgcca tccataatcg ctacacaaga
     6421 gttggtagta cccaggtcga taccaattat tttacccatc taaacgtctc cactatatat
     6481 tcggtcatca tgtggttgtg agtctgtaat aggggcgaaa cgtctggttt caactgccca
     6541 atttcatttt tttgcagact cactcactgc ggttgactac taaatggggt cgtaacccac
     6601 gtcatcaagg gggagatgca aaaatttttt gtgcttttgc gcagaaattt cgcgatatgg
     6661 caggacaaga cgtaaaattg acagcaattt tattcatata aagaatgaat cgcagccaag
     6721 gcgttcatca aaaaattgta ataaaaagaa aagattacgt gcctgaatct tctctttatc
     6781 agcagtaaac tagtgggtat tcatccccct acctcttccc actaagagaa tccttatgaa
     6841 atccgttttt acgatttccg ccagcctggc gattagcctg atgctgtgct gcacggcgca
     6901 ggcaaacgac cataaactcc tcggcgtcat tgcaatgccg cgtaacgaaa ccaacgatct
     6961 ggcgctgaaa cttcctgttt gtcgcattgt gaaacgcata caactctctg ccgaccatgg
     7021 cgatttacag ttaagtggtg catcggttta tttcaaagcc gcccgtagcg ccagtcagag
     7081 cctgaatatt ccttcagaaa taaaagaagg gcaaaccact gactggatca acattaacag
     7141 cgataacgac aataaacgct gcgtctcaaa aatcaccttt tccggtcata cggtgaactc
     7201 atcggatatg gccacgctga aaattatcgg cgacgactaa cgccagaata tagaagccac
     7261 aaaaaatgaa tgttaattac ctgaatgatt cagatctgga ttttcttcag cattgtagtg
     7321 aggaacagtt ggcaaatttc gcccgattgc tcacccataa tgaaaaaggc aaaactcgcc
     7381 tctccagcat actgatgcgc aacgaactgt ttaaatcgat ggaagggcat cccgagcaac
     7441 atcgccgcaa ctggcagctg attgccggag aattacagca ttttggtggc gatagtatcg
     7501 ccaacaaact gcgcggacac ggtaaattgt atcgggccat tttgctcgat gtttcaaagc
     7561 gattgaagct gaaagccgac aaagagatgt ctacgtttga aattgagcaa cagttactgg
     7621 aacaatttct gcgtaatacc tggaagaaaa tggacgagga acataagcag gagtttctgc
     7681 acgcggtcga tgccagggtg aatgagctgg aagagctgct gccgctgctg atgaaagaca
     7741 aattattggc aaaaggtgtg tcgcatttgc tttccagcca actgacccgc attttacgca
     7801 cccacgcagc aatgagcgta cttgggcatg gtttgctgcg cggcgcgggg ctgggaggcc
     7861 ctgtgggcgc agcattaaat ggggttaaag cggtcagcgg cagcacctat cgcgtgacga
     7921 ttccagccgt actgcaaatc gcctgcctgc gccggatggt tagcgccact caggtctgat
     7981 accggaaacc ggggaaaatt ttcattttcc cctcaaaaga tcgtagacac tgccccactg
     8041 gctgattatt atgccgcgcc ctgaaaacac tacagttatt cagggaaatt atttcaccat
     8101 tcattcgatg atgatttttg aggaattatg ggcaacacta agttggctaa tccggcaccg
     8161 ctgggcctga tgggcttcgg catgaccacc attctgctta acctgcacaa cgtgggttat
     8221 ttcgctctgg acggtattat tcttgccatg ggcattttct acggcggcat cgcgcaaatt
     8281 tttgctggtc tgctggagta caaaaaaggc aacactttcg gtttaaccgc attcacctct
     8341 tacggttctt tctggctgac gctggttgcg attctgctga tgccgaaact gggtctgacc
     8401 gatgcgccaa atgcacagtt ccttggtgtc tacctgggtc tgtggggcgt atttacgctg
     8461 tttatgttct tcggcactct gaaaggcgcg cgcgtcctgc aattcgtttt ctttagcctg
     8521 accgtgctgt ttgccctgct ggcgatcggt aacattgccg gtaatgccgc gatcatccac
     8581 tttgccggct ggattggtct gatctgcggt gccagcgcaa tctatctggc gatgggtgaa
     8641 gtgctgaacg agcagtttgg tcgcaccgtt ctgccgattg gtgaatccca ctaattgtct
     8701 tgagatcccc ccgctcgggg ggattttttt attcgctaac gtcgcgtctt gcactcttcg
     8761 gtctgaatgc agcaaccact tctggtgccg tttcaacgta tggcccttcc agcaactgaa
     8821 tgcagtacgg tacgctggca aaaataccat gcaccacaac gttaccctca gcgtccttca
     8881 caccttccag cgtctcttta atagacttcg gctgaccggg taagttaagg atcagcgcct
     8941 gtttgcgaat cacgcccacc tgacgcgaaa ggatcgcagt tggtacaaaa tgcaggctga
     9001 tctggcgcat ctgttcacca aagccaggca tctcgcggtc cgctactgcc agcgtcgcat
     9061 cgggcgttac gtcacgacgc gccgggccag ttccgcccgt ggtgagcacc agatggcaac
     9121 tcatttcatc caccagctca cacaacgttt gctcgatgat cgcctgctca tcggggatta
     9181 agcgggtttc cagttcaaac ggcgtggtta gcgccgatgt cagccattct tccagcgctg
     9241 gaatgccttt atcctgataa acgccgctgg atgcgcgatc agagatggaa actaagccaa
     9301 tacgtaaagt attcatcaag atttttccgt ttaaacaatc tcgttaaaca gaatgataca
     9361 ctgcgaaggg agtgacagac aggcttcaga agaggtagcg tgaccgactt cccggtcacg
     9421 ctaagaatga ttacagcaga tcgccgatca ttttttccag tttttcctgg tcaatagcaa
     9481 acttacggat accttccgcc agtttatcta ctgccattgg atcctggttg tgctgccaca
     9541 ggaactcgga ctcagtgata cgcgccggac gcgctttcac ttcgccagtg taagacagtt
     9601 tacgttcgat agccccttcg ctctccgcca gctctttcag cagtgccggt gcgatggtca
     9661 gacggtcaca gcctgccagt tccagaattt cgccgatgtt acggaagctt gcgcccataa
     9721 ccacggtttc ataaccgtgc tctttgtagt actggtagat ttcagataca gaaaccacgc
     9781 ccggatcttc tgccggagcg tactctttct tatcggtatt cgctttgtac cagtcaagaa
     9841 tacggccaac aaacggcgag atcaggaaca cgcccgcctc cgcacaagca cgagcctgag
     9901 cgaaggagaa cagcagggtc aggttacagt tgataccttc tttttccagc tgttctgcag
     9961 cacggatacc ctgccaggta gaagccagtt tgatcagaat acgatcgttg ctaatacctg
    10021 catcgttgta gagtttgatc aggcgttttg ctttcgcaat tgacgcttca gtgtcatagg
    10081 aaagacgcgc atcaacttca gttgagatac ggcccggaac cagtttcagg atttccagac
    10141 caatatttac tgccagtttg tcggtcgcgt ccacgatctg ctgcgcgcga tcgttgctct
    10201 gctgtttcgc ccaggcgaca gcatcatcaa tcaacttacg gtattccgga atctgcgctg
    10261 cgttaagaat gagagaaggg ttggttgtgg catcctgcgg ttgatacagc ttcattgccg
    10321 cgatgtcccc agtgtcggcc actacggtgg tgtactgacg aagggaggtc aatttgtccg
    10381 tcatgatagt atttctcttt aaacagcttg ttagggggat gtaaccggtc tgccctgatg
    10441 atatcacgac gcaacgtcag cgcaaccgcc gacaagacgg ttatcgccag gcgagactgt
    10501 ttcggatttc tgacactgcg ttgatatcgc ccaccatttt tatacaaaac ctcatgtatg
    10561 ctacgcagaa gttatcaagt acctcgtagc gtatatactt cttaaacaat tggtaacgtt
    10621 tacacaggaa agtcatcgcg accggcaata agagggatat gcatgccaga ttttttctcc
    10681 tttattaaca gcgtcctttg gggatcggta atgatttacc tgctcttcgg cgcaggttgt
    10741 tggttcactt ttcgcaccgg atttgtgcag tttcgctaca tccgccagtt tggcaaaagt
    10801 cttaaaaata gcattcatcc acagccaggc ggtttaacct catttcagtc attgtgtacc
    10861 agtcttgcgg cgcgcgtggg tagcggcaac ctggccggcg ttgcgctggc aattaccgcc
    10921 ggtggaccag gtgccgtctt ttggatgtgg gttgctgcgt ttatcggcat ggcgacctcg
    10981 tttgccgaat gttcccttgc acaactttat aaagaacgtg acgtcaatgg gcagtttcgt
    11041 ggcggaccgg catggtatat ggcgcgcggg ctggggatgc gctggatggg cgttctgttc
    11101 gccgtctttt tgctcatcgc ctacggcata attttcagcg gagttcaggc gaacgccgtt
    11161 gcccgagccc tgagtttttc ttttgatttt cctccgctgg tgacgggcat tattctcgct
    11221 gtctttgctc tgctggcaat cactcgtggt cttcatggcg tcgcccggct catgcagggg
    11281 tttgtcccgt tgatggcgat aatctgggta ctgaccagcc tggtgatttg cgtaataaat
    11341 atcgggcaac ttccccacgt catttggtct atttttgaga gtgcttttgg ctggcaggaa
    11401 gcggcaggcg gcgcggcggg atatacctta agccaggcga ttactaacgg ttttcagcgc
    11461 agtatgtttt ccaatgaggt gggaatggga tcgacgccaa acgcggcagc ggcagctgca
    11521 tcctggcctc cgcatccggc agcacaaggg attgtccaga tgattggcat ttttatcgac
    11581 accctggtca tctgtacggc aagcgccatg ctgatattac tggcgggtaa cggcacaacc
    11641 tacatgccgc tggaaggtat tcagcttatc cagaaggcga tgcgggtgct aatgggttcc
    11701 tagggtgctg aatttgttac cctcgtggtt attctgtttg ccttcagctc catcgttgcc
    11761 aactacattt atgccgaaaa caatctcttc tttttacgcc tgaacaaccc taaagcgatc
    11821 tggtgtttgc ggatctgcac cttcgcaacg gtcatcggcg gcaccttgct aagccttccg
    11881 ctgatgtggc aactggcaga tatcataatg gcctgcatgg ctattaccaa tttgaccgcc
    11941 attttactgc tctcgcctgt ggttcatacc attgccagtg attatctacg ccagcgtaaa
    12001 ctcggcgtgc gcccggtgtt tgatccgttg cgttatccgg atatcggtcg ccagctttct
    12061 ccggacgcgt gggatgatgt ttcgcaggag taatcacaac tatcgatcaa ctcattctct
    12121 ttttttgcta aagtcggcat aaatttcctg caaggactgg atatgctgat tcttatttca
    12181 cctgcgaaaa cgcttgatta ccaaagcccg ttgaccacca cgcgctatac gctgccggag
    12241 ctgttagaca attcccagca gttgatccat gaagcgcgga aactgacgcc tccgcagatt
    12301 agcacgctga tgcgcatcag cgacaaactg gcgggtatca acgccgctcg ctttcatgac
    12361 tggcagccag atttcacgcc ggcgaatgcc cgccaggcga ttctggcgtt taaaggtgat
    12421 gtctacaccg gcttgcaggc cgaaaccttc agcgaagacg atttcgattt tgcccaacag
    12481 catttgcgaa tgctttccgg cttgtatggc gtactgcgcc cgctcgattt aatgcagcct
    12541 tatcgtctgg aaatggggat ccgtcttgag aatgcccgag ggaaagatct gtatcaattc
    12601 tggggagata tcatcaccaa caagctgaac gaggcgctcg cagcacaagg cgataatgtg
    12661 gtgattaatc tggcgtcaga tgagtatttt aaatcggtga agccgaagaa attgaatgcc
    12721 gagattatca agccggtgtt cctcgatgag aagaacggca aatttaagat catcagcttc
    12781 tacgctaaga aagcacgcgg tctgatgagt cgtttcatta ttgaaaatcg gctgaccaaa
    12841 ccagagcaac tgactggttt taatagcgaa ggttacttct ttgatgaaga ttcctccagc
    12901 aatggcgaac tggtgtttaa acgctacgag cagcgttaag tttgtagtca atgccggatg
    12961 cggcgtgaac gccttatccg gcctacaaaa gcatgcagat tcaatatatt gcagaattaa
    13021 cgtaggcctg ataagacgcg ccagcgtcgc atcaggcgct gaatgccgga ttgttacccg
    13081 gcatttgtca tttagcggtg atgtttgcct ggaccatgac cgccgtgatg atcatgagga
    13141 gctttcttat ggtggcgcgg cggtggcggc ggtccgtgtg ggtgccagcg attgcctcgc
    13201 cattcataat gttgtttcca ccagccgtgg tcgcgccagt gacctccatc ccagtaatag
    13261 ccacgattat cacgatcgcc tatctgtaat tttactgacg ggactaacgt aatttccgca
    13321 gcctgtgctg ccatgggagc gaccagaacc agggaaagtg cgagtacgat agattgcatc
    13381 tttttcacag gttactcctt ttatcattgc ccgttatggg tcgatatccg gagattacgc
    13441 gatgagaacg acggttgtta ttctccgcaa tcctaatctc taagttaccg catttattga
    13501 taatttctcc tttttccctg tcgtttttct ccataatttc ttcataaaaa agccgggctg
    13561 cataaaagca aacccggcct gattgagata atgaatagat tttactgatg attcatcatc
    13621 aatttacgca acgcagaaaa atcggcgggc agattatgtg aaagcaaggg taaatcagca
    13681 cgttctgcca gctcttttgg cagatccaac gtttcaccga gaatcgcttc cacgctctct
    13741 ttaaatttcg ccggatgcgc ggtgccgagg aacaagccat attcgcctgg attcaactgg
    13801 tcacgcagcg cacgatacgc tacggcagcg tgcggctccg aggtgtagcc cagttctttt
    13861 aactcacgca ttgtctgttg cgtggtttca tcatccacgg ctgcataacc cagctctttc
    13921 agttgccaga ttttgcggcg gaacaactct tccacacgcg gccagttgtt cggctgactc
    13981 acgtccatcg cgttggataa cgtcgcctga gtcgctttgg gtgaccactg accgtcgtgc
    14041 aggaaacgtg gcacggtatc gttcacgttg gtcgcagcaa taaaacgttt caccggcaga
    14101 ccgaatgact tcgccagcag acccgccgtc aaatcgccga agtttccgct tggcaccgag
    14161 acaaccagct ggttgcgcgc ctcctgcggc agctgcgcaa cagcttcaaa gtagtagcaa
    14221 atctgcgcca gcaaacggct gatgttaatc gagttagccg agtttaaccc cagcgccact
    14281 ttcagttctt catcatcaaa cgcctgcttc accagcgcct gacaggcatc gaaatcgccg
    14341 tcgatggcaa cagtttcgat attgccgccc aacgtacaga acagtttttc ttgcagtgga
    14401 ctgattttgc ctcgtggata gaggataacc actttcacat tcggcaaacc gtagaaagca
    14461 tgagccactg cagctccggt atcaccggag gtcgcggtca gaatggtcac tggcttatca
    14521 tccgcaatat gggtcagcat ttgtgccata aagcgaccgc cgaaatcttt aaatgccagc
    14581 gttggcccgt ggaacaattc cagacaaccg acatcgcttt caacattggc gaccggagcc
    14641 gggaaggcaa acgccgcgcg cacgcgctct tccaggattt cctgtgggat ttcatcacca
    14701 ataaacgccg agaggatctt cgcactgcgg gtgacaaaat ccagcttcag catctcatca
    14761 atttcagtca ggctgaattc cggcaggtcg tgcggaaaaa acagcccctg atttttgccc
    14821 aacccctggg ttacggcttg cgcaaagctg acctgctcgt tgtgatcttt cagattgtag
    14881 agtttcattt agttttccag tactcgtgcg cccgccgtat ccagccggca aatatgaaca
    14941 aaaccttcct gattttgcag gtagttctta cccaaccagt cggcaacgcg ctgggcggta
    15001 tccggcttgt cacatagagc gaacaaggtc gggccggagc cggagatacc gctcgctacc
    15061 gcgccgattt ccgcgaccgc ctgccgcgcc tgccggaagc caggcagtaa ccgttcacgg
    15121 tagggttcag cgataacatc tttcatcagc ttcgcggcaa gctcaggctg acgggaatag
    15181 caggcgtgaa tgaagccagc cagatgtcgc ccgtgcgcaa tgcaatcctg gcggcgatac
    15241 tgcgccggta aaatagcccg ggcttctgcc gtcgagactt taattcccgg atacgccagc
    15301 acccacagcc actcatcaaa ccctggcact tgctggctga tgatgtcgtt ttcttcgatc
    15361 atcaactgca taccaccaag aaaacacggt gccacgttgt cgtaatgaat gctgccggag
    15421 atacgtcctt ccagctcgcc catcaaagcc agcaaacgag tgtcattaag cggcttgccg
    15481 cagtgttcat tcatcgccat cagcgccgcg accaccgaac aggcgctgga gcctaagccc
    15541 gaaccgatcg gcatattctt ttccagagtc atcgccactg gaatttgctt gccaagctcc
    15601 tggcaaaaac gctcccagca ctgataaacg atattttccc gtggttctga cggcagctta
    15661 tcggcaaagc gtccgagttt gttgagactg aatgtctctg ccgcctcaac cgtgactaca
    15721 tctccgagca atgcaccatc aacgggtgcc accgccgccc cgagcacatc aaacccgacg
    15781 ctcatattgg cactggaagc cggggcataa actttaacca tgtcagactc ctaacttcca
    15841 tgagagggta cgtagcagat cggcaaagac accggcagcg gtaacgtcat tgcccgcacc
    15901 atatccgcgc agcaccaacg gcagcggctg ataatagtgg ctataaaagg ccagggcgtt
    15961 ttcgccattt ttcactttga acagcggatc attcccatcc acttcggcaa tcttcacgcg
    16021 gcagacacca tcttcatcaa tattgccaac atagcgcaaa acttttcctt catcacgggc
    16081 cttcgccacg cgcgcggcaa agagatcgtc gagctgtgac agattcgcca taaaagcggc
    16141 aacatcaccc tcggcgttaa actctgcggg cagcacaggt tcaatttcaa tatccgccag
    16201 ctccagttca cgtcccgttt cacgagcgag aatcaatagt ttacgcgcca catccatacc
    16261 agaaagatca tctcgcgggt ccggttcggt ataacccatt tcccgcgcca gcgtggtcgc
    16321 ctcggagaaa ctcatgcctt cgtctaactt gccgaagata taagaaagcg aacctgaaag
    16381 aatgccggag aacttcacca attcatcacc tgcattgagc agattttgca ggttctcaat
    16441 aaccggtaat ccagccccaa cgttggtgtc atagaggaat ttacgccgcg atttttccgc
    16501 cgcataacgc aactgatgat agtaatccat cgacgaggtg ttggcctttt tgttcggcgt
    16561 gacaacgtgg aaaccttcgc gcaggaagtc ggcatattga tccgccactg cctggctgga
    16621 ggtgcagtca acaatgaccg ggttcagcag atgatattct ttcacgaggc gaattaagcg
    16681 cccgagatta aacggctctt tggcttgtgc aagttcttcc tgccagtttt ccagattaag
    16741 gccatgcaca ctagtgagca gagccttcga gttggcaacg ccgcagacac gtaagtcgat
    16801 atgtttattc ttcagccagc tttgctgacg cttcagttgc tccagcagcg caccgccaac
    16861 gccaccgacg ccaatcacaa acacttcgat aacctgatcg gtattgaaca gcatctgatg
    16921 agtaacgcgc acgccagtgg tcgcatcatc gttatttacc acgacagaga ttgagcgttc
    16981 agaagatccc tgagcaatgg cgacaatgtt gatattggcg cgggccagcg cggcaaagaa
    17041 tttcgccgag atcccacgca aggtgcgcat accatcacct accaccgaga taatggccag
    17101 ccgttccgtc accgccagcg gctccagtaa gccttctttc agttccaggt agaactcttc
    17161 ctgcattgcc cgttcagctc gcacacagtc gctttgtgga acgcagaaac tgatactgta
    17221 ttcggaagat gattgcgtaa tcagcaccac ggaaatacgg gcgcgtgaca ttgcagcaaa
    17281 gacgcgcgcc gccatgccaa ccatcccttt catccccggg ccggaaacgc tgaacattgc
    17341 catgttattc agattggaaa tgcccttgac cggtaattcg tcttcatcac ggctggcacc
    17401 aatgagcgta cctggtgctt gaggatttcc ggtattttta atcaggcaag ggatctggaa
    17461 ctgggcgatg ggggtaatgg tgcgggggtg aagaacttta gcgccgaagt aggaaagctc
    17521 catcgcttcc tggtaggaca tcgacttcaa caacctcgca tcgggcacct gacgcgggtc
    17581 gcaggtatag accccgtcaa cgtccgtcca aatctcgcaa caatcggcgc gtaaacaggc
    17641 agccagcacc gccgcggagt agtcggaacc gttacgtcca agcaccacca gttcgccttt
    17701 ttcattaccg gcggtgaaac ctgccatcaa caccatgtga tcagccggaa tgcggcttgc
    17761 cgcaatacgg cgggtggact cagcaatatc gacggtagat tcgaggtaat gccccactgc
    17821 cagcagtttt tcgaccggat cgataacggt aacgttgtga ccacgcgctt ctaacacgcc
    17881 ggccataatg gcgatcgaca ttttctcgcc acggcaaatc agcgcagcgt tgatgctatc
    17941 cgggcactgc cccaacaaac taatgccatg caggacatgt tttatttggg caaattcctg
    18001 atcgacgaaa gttttcaatt gcgccagcgg gaaccccggc tgggcggcgg cgagtcccgt
    18061 caaaagttcg gcaaaaatac gttcggcatc gctgatattg ggtaaagcat cctggccgct
    18121 aatggttttt tcaatcatcg ccaccaggtg gttggtgatt ttggcggggg cagagaggac
    18181 ggtggccacc tgcccctgcc tggcattgct ttccagaata tcggcaacac gcagaaaacg
    18241 ttctgcattt gccactgatg taccgccgaa cttcaacact cgcatggttg ttacctcgtt
    18301 acctttggtc gaaaaaaaag cccgcactgt caggtgcggg cttttttctg tgtttcctgt
    18361 acgcgtcagc ccgcaccgtt acctgtggta atggtgatgg tggtggtaat ggtggtgcta
    18421 atgcgtttca tggatgttgt gtactctgta atttttatct gtttgtgcgc tatgcctata
    18481 ttggttaaag tatttagtga cctaagtcaa taaaatttta atttactcac ggcaggtaac
    18541 cagttcagaa gctgctatca gacactcttt ttttaatcca cacagagaca tattgcccgt
    18601 tgcagtcaga atgaaaagct gaaaaatact tactaaggcg ttttttattt ggtgatattt
    18661 ttttcaatat catgcagcaa acggtgcaac attgccgtgt ctcgttgctc taaaagcccc
    18721 aggcgttgct gtaaccagtc gaccagtttt atgtcatctg ccactgccag agtcgtcagc
    18781 aatgccatga ctcgttcgcg taaagcttgc agttgatgtt ggtctgccgt tgtgtcactt
    18841 ttcgccggtt gttgtattaa tgttgctaat tgatagcaat agaccatcac cgcctgcccc
    18901 agattgagcg aaggataatc cgccaccatc ggcacaccag taagcacgtc agccaacgct
    18961 aactcttcgt tagtcagccc ggaatcttcg cgaccaaaca ccagcgcggc atggctcatc
    19021 catgaagatt tttcctctaa cagcggcacc agttctactg gcgtggcgta gtaatgatat
    19081 ttcgcccgac tacgcgcagt ggtggcgaca gtgaaatcga catcgtgtaa cgattcagcc
    19141 agtgtcggga aaactttaat attatcaata atatcaccag atccatgtgc gacccagcgg
    19201 gtggctggct ccaggtgtgc ctgactatcg acaatccgca gttcgctaaa acccatcgtt
    19261 ttcattgccc gcgccgctgc cccaatattt tctgctctgg cgggtgcgac caggataatc
    19321 gttatacgca tattgccact cttcttgacc aaataaccgc gaacagggtg atcactgtca
    19381 acttattacg cggtgcgaat ttacaaattc ttaacgtaag ccgcagaaaa agccctttac
    19441 ttagcttaaa aaaggctaaa ctattttctg actgcactaa cggtttaatt gttaaaaaat
    19501 gctacatatc cttctgttta cttaggataa ttttataaaa aataaatctc gacaattgga
    19561 ttcaccacgt ttattagttg tatgatgcaa ctagttggat tattaaaata atgtgacgaa
    19621 agctagcatt tagatacgat gatttcatca aactgttaac gtgctacaat tgaacttgat
    19681 atatgtcaac gaagcgtagt tttattgggt gtccggcccc tcttagcctg ttatgttgct
    19741 gttaaaatgg ttaggatgac agccgttttt gacactgtcg ggtcctgagg gaaagtaccc
    19801 acgaccaagc taatgatgtt gttgacgttg atggaaagtg catcaagaac gcaattacgt
    19861 actttagtca tgttacgccg atcatgttaa tttgcagcat gcatcaggca ggtcagggac
    19921 ttttgtactt cctgtttcga tttagttggc aatttaggta gcaaacatgc agaccccgca
    19981 cattcttatc gttgaagacg agttggtaac acgcaacacg ttgaaaagta ttttcgaagc
    20041 ggaaggctat gatgttttcg aagcgacaga tggcgcggaa atgcatcaga tcctctctga
    20101 atatgacatc aacctggtga tcatggatat caatctgccg ggtaagaacg gtcttctgtt
    20161 agcgcgtgaa ctgcgcgagc aggcgaatgt tgcgttgatg ttcctgactg gccgtgacaa
    20221 cgaagtcgat aaaattctcg gcctcgaaat cggtgcagat gactacatca ccaaaccgtt
    20281 caacccgcgt gaactgacga ttcgtgcacg caacctgctg tcccgtacca tgaatctggg
    20341 tactgtcagc gaagaacgtc gtagcgttga aagctacaag ttcaatggtt gggaactgga
    20401 catcaacagc cgttcgttga tcggccctga tggcgagcag tacaagctgc cgcgcagcga
    20461 gttccgcgcc atgcttcact tctgtgaaaa cccaggcaaa attcagtctc gtgctgaact
    20521 gctgaagaaa atgaccggcc gtgagctgaa accgcacgac cgtactgtag acgtgacgat
    20581 ccgccgtatt cgtaaacatt tcgaatctac gccggatacg ccggaaatca tcgccaccat
    20641 tcacggtgaa ggttatcgct tctgcggtga tctggaagat taatcggctt taccaccgtc
    20701 aaaaaaacgg cgctttttag cgccgttttt atttttcaac cttatttcca gatacgtaat
    20761 tcatcgtccg ttgtaacttc tttactggct ttcattttcg gcagtgaaaa cgcataccag
    20821 tctatattac gagtcacaaa catcatgccg gccagcgcca ccaccagcac actggttccc
    20881 aacaacagcg cgctatcggc agagttgagc agtccccaca tcactccatc cagcaacaac
    20941 agcgcgaggg taaacaacat gctattgcgc cagcctttca ataccgcctg caaataaata
    21001 ccgttcatta acgccccaat cagactggcg attatccatg ccacggtaaa accgatatgt
    21061 tcagaaagcg ccagcaagag caaataaaac atcaccaatg aaagccccac cagcaaatat
    21121 tgcattgggt gtaaacgttg cgcggtgagc gtttcaaaaa caaagaacgc cataaaagtc
    21181 agtgcaatca gcagaatggc gtacttagtc gcccggtccg ttaattggta ttgatcggct
    21241 ggcgtcgtca ctgcgacgct aaacgcgggg aagttttccc agccggtatc attgcctgaa
    21301 gcaaaacgct caccgagatt attagcaaac cagctgcttt gccattgcgc ctgaaaacct
    21361 gactcgctag cttcccgttt ggctggtaga aaatcaccta aaaaactggg atgcggccag
    21421 ttgctggtta aggtcatttc gctattacgc ccggcaggca ccacagaaag atcgccggta
    21481 ccgcttaaat tcagtgccat attcagctgc aggttctgct tccgccagtc cccttcgggt
    21541 aaagggatat gcacgccctg cccgccttgc tctaacccgg taccgggttc aatggtcagt
    21601 gccgttccgt taacttcagg cgctttcacc acaccaatac cacgcgcatc cccgacgcta
    21661 atcacaataa agggcttgcc taagatgata tttggcgcgt tgagctcgct aagacgcgaa
    21721 acatcgaaat cggcttttag cgttaaatca ctgtgccaga cctgaccggt ataaatccct
    21781 atcttgcgtt cttccacgtt ctgattgcca tcaaccatca atgactcagg taaccaaaaa
    21841 tggataaaac ttcgtttccg ctccacggtt ttatcctctt cctgcaccgt ataaagctcg
    21901 gtcacaggaa tagcgataag cggcccaacg agtttttgcg gcccgctggt actttggcga
    21961 atcgcatctt ccacatcgct acggtaatcg gcacgttcga caatcacttg ccgaatcagc
    22021 attatcggaa tcaacaacag caatactgca ccaaacaagg tggtcatttt ccagaacagg
    22081 ggggatttca acatagtctt ctcctttgca atggcagcag gatacgaagg ctatgtggga
    22141 agaatttgaa gctatgtgaa gtgacggtga agtcgtagcg aggccagcac gccaccttcc
    22201 tgcacgttgc gcagcgtgac ttcgccgtta aacaaacggg cgacctcact gacgaacgcc
    22261 aaccccagac cgctgctttt ttgcccattt gcacgcggca aagagtaaaa gcgttcaaaa
    22321 atacgtgaca gcgcgtagtc aggaatacca ctaccggtat ccagcacctt aagcgtgacg
    22381 tgttcctgat ccacttcggc gcttagcgtt atgcaaccgc tctcgggagt aaaatcgatg
    22441 gcgttatcca gtaaattccc cagcgcctga tccagtaacg ccggttcagc agcaacatta
    22501 acctcggtgg gcataacatg caaagtgatg tttttttctg ccaactgcac ggtgcgcgct
    22561 tcgctgacgc ggcgaaataa tgccgccaca tcaacagcag tcagaacgac ttcctgacga
    22621 ttctccagtc ttgcctggcg tagtaacgtt tccaccagcg cctgcattcg cgcattttgt
    22681 gtcagaatgt tgtcagtaaa acgagccact acttcaggcg gcggaccttc gcgtaaaatt
    22741 tccgccgcgc cacgaatcgc cgccagtggg ctttttagct catgggttaa cgcataaaca
    22801 tactgctcaa tatagttttt cccttccagc ttcacgcgca tactttccag cgcctgcgcg
    22861 agtttacgca gctcgctact accgagatcg ggaagaggaa cgggcttatt gtcagtgacg
    22921 gaatcagcat agcgagtgag ccgggcaatg gagcggttga tccaccaaac catgcctgcg
    22981 ccaatcacca gtgcaatccc caacaaaatg gcgctggccc ataaaattcg ccgctcgctg
    23041 cgtttaatga ccggagccat cgccgcgttc ggtttgccta cgctcaaaac gccaataagc
    23101 cgcgagccgc ccataatcgg cgcggcaaca tacatcaccg aactttcggg atcggcagga
    23161 ttttgcaacg tgctgcgcgc accatactga ccacgcaacg ttagccagac gtcattccag
    23221 cgcgaataat cctgtccaac ggctttattt gccgaatcga acaatacttt gccatgcgca
    23281 tcggtcatat agacatgata ttcattgcgc actttgttaa tgccaccaat attggcgcga
    23341 aacgggcgat gttgtagctg attaaacgcc tgcgccagtt gcccatgcgt tgggtcccca
    23401 gagagcaaat cgggacgcgc cagctccgca agtaacgttg ccgtgtcgat caacgtcccc
    23461 tccgttgctc ttcgcacgcc cggtttaact tctttgacaa aaatagccag tacgaaccag
    23521 gccgccaccg ccaccagtaa aaaatagccc agcagcaacc gcatgccgat acgcattaca
    23581 agcccctcag gctatatccc atgccgcgat gagtattaat cggtgaaaga tcggggttga
    23641 tggcgcgcag cttggcacgc agcgttttaa tgtggatatc gacggtgcga tcgtaggtgt
    23701 cctgcgcatc ttcccacacg ctatccatta gttgctggcg ggaccagacg cggcctggcg
    23761 acttgagtaa cgtcttcagc aataaaaact cataccgcgt cagcgttaat ggcgtgtcaa
    23821 accagctgat ctgcgccgcg ggttcattca attcaaaatg accaatacgg atgacgggag
    23881 acggcgtcga gaacttcttc acccgacgca gtaaggtgcg caccctggcg cacacttcgc
    23941 ggggtgaaaa cggtttagcc acatagtcgt cagcgccaat ttccagccca agcaggcgat
    24001 cgacctcttc acttcgggcc gtcaggaaca gtacaggtaa cgccggatag agcgccagta
    24061 actggcggca caattcaaag ccactgatat caggcaaacc aacgtcgaga atcatgacgt
    24121 cgggaacctg ctggcgagct ttatccagca ccggcaaacc tcgctcaaag acctcgacgg
    24181 caaaaccttc ctgctgcaac atgtagacca gcgtgtcggc tatcccttgc tcatcttcca
    24241 ctaaccagac cgtttcccgt tgcataaatc gcctctgtta ttgccgccac ggcatgacag
    24301 gaaccgcgct aatcgcgttt ttcggcgaac cttctacaac tttgtcggag taagccagat
    24361 aagcgagcgc gttgcgtttg gcgtcataaa agcgcacgac ctgtaacgac ttaaagacca
    24421 gggacgtgcg ttttttgaat actacctcgc cctgagcttt gccgttttta atacgatccg
    24481 acagttcaat cggcccgact tgctgacaag aaatggccgc atcggaggta tcttctgcca
    24541 ggcccaatcc ccctttaata ccaccggttt tcgcccggct cacataacag gtgacatttt
    24601 tcacatcggg atcgtcaaag gcttccacaa caattttgtg atccgggccg atcattttaa
    24661 atacggtgtc gaccgaacca atctcttctg catgagccaa tggccccagc attatcaggc
    24721 ttaaagacag gatcaaatgc ttgtatttca tattgttacc atttttatct attacacgag
    24781 caattagttc gtcacggtaa ataacatatt gattaagaac aaaatctcaa ttcttttatt
    24841 tccgtcaagc cctaaaacat actctactaa gaaaaaaaca ctgaatgcta aaacagcaaa
    24901 aaatgctatt atccaattac ctgatgtcag gtgctcgttg ttgaaaggat gaggatattt
    24961 tatggatcag gccggcatta ttcgcgacct tttaatctgg ctggaaggtc atctggatca
    25021 gcccctgtcg ctcgacaatg tagcggcgaa agcaggttat tccaagtggc acttacagag
    25081 aatgtttaaa gatgtcactg gccatgctat tggcgcgtat attcgtgctc gtcgtttgtc
    25141 gaaatcggcg gtcgcactac gcctgactgc gcgtccgatt ctggacatcg cgctgcaata
    25201 ccgcttcgac tctcaacaga catttacccg cgcattcaag aagcagtttg cccagactcc
    25261 tgcactttac cgccgttctc ctgaatggag cgcctttggt attcgcccgc cgctacgtct
    25321 gggtgaattc acaatgccag agcacaaatt tgtcaccctg gaagatacgc cgctgattgg
    25381 tgttacccag agctactcct gttcgctgga gcaaatctct gatttccgcc atgaaatgcg
    25441 ttatcagttc tggcacgatt ttctcggcaa cgcgccgacc attccgccgg tgctctacgg
    25501 cctgaatgaa acgcgtccga gtcaggataa agacgacgaa caagaggtat tctataccac
    25561 cgcgttagcc caggatcagg cagatggcta tgtactgacg gggcatccgg tgatgctgca
    25621 gggcggcgaa tatgtgatgt ttacctatga aggtctggga accggcgtgc aggagtttat
    25681 cctgacggta tacggaacgt gcatgccaat gctcaatctg acgcgccgta aaggtcagga
    25741 tattgagcga tactacccgg cagaagacgc caaagcggga gatcgcccaa ttaatctgcg
    25801 ctgtgaactg ctgattccta tccgccgtta acgctgcagc tcatctaatg caggggcgtc
    25861 cagatgcgag atgtcccctg cggtttcaac gacccagcca gacgccagcc acaggctttc
    25921 ctgataatcc acgcgcgaaa tagaacagtt acgcagacgt aagcggcgct ctgcccatgc
    25981 tggtaatccg agaatcgtac tcaccagaca tcccagtgca ataccatggc tcaccagcag
    26041 cggtcggctt ccctgcggta agtcccggca agattccagt gccgcattga cgcgatcgct
    26101 gagctcctgc attgactcgc cttcaggaat acgcccgtca acggtgccat tgaccagctg
    26161 ccgacgccag ttctcttctt cttcggtcag agaatcgata tgtctttttt ccagcacacc
    26221 catgtttaat tcacgcaggc gagaatcaaa gatgatgtca cagccgcagg cctgggcgat
    26281 gatttccgcc gtacgccggg tgcgtcctaa atcgctactg ataatatgcg taatgccaag
    26341 ctctttggca cgggttgcca cctgcatcgc ctgttgctca cctttggcgg tcagcgggct
    26401 gtcagactgg ccctgaatac gtcgctcggc gttccactgc gtttcaccgt ggcggactag
    26461 gtatacctgt aacatgcttt ttttccgtat actgctgtaa cgttaatttt ctgagtaatg
    26521 ctgattatgc accaagttgt ctgtgcgacc accaatcccg ctaaaattca ggccattctg
    26581 caggcatttc acgagatctt cggcgaagga tcctgccata ttgcatccgt tgccgtcgag
    26641 agcggtgtac cggaacaacc ctttggcagt gaggaaacgc gcgctggcgc acgaaatcgg
    26701 gtagcaaatg cccgccgttt acttccagag gctgattttt gggtggcgat tgaagctggc
    26761 attgatggcg acagcacttt cagttgggtg gtgattgaaa acgccagcca gcgcggcgag
    26821 gcgcgttcgg caactttgcc gttacctgcg gtgattctgg aaaaagttcg tgaaggcgag
    26881 gcgcttggtc ccgtgagtcg cgttataccg gtattgatga gattggtcgt aaggaagggg
    26941 cgatcggcgt gtttaccgcc gggaaactca ctcgcgccag cgtctatcac caggcggtga
    27001 tcctggcgct gagtccgttt cacaatgccg tgtatcaggc attgtaggac ggataaggcg
    27061 ttcacgccgc atccgacatt cggtgcacga tgcctgatgc gacgctgccg cgtcttatca
    27121 ggcctacaaa atcaatcgct tttcagcaac acctcttcca gccactgacg cagctccacg
    27181 ggcgcggctt tcaggctgtt agatccacgc gtaatcgtcg cgatgcccgc gccgagttca
    27241 ttttttaact cacgctgact catttcgcca cgcaacagct cttcgacaat acgcacgcga
    27301 gtccccaacg cttcgcgctc atctggcgtc agcatcaggt ttaacaacgg taaatggaga
    27361 tcgttttggt aggcattctt aagcaggtcg acaaaacgta accactcctg gtgacgctgt
    27421 tctgccatcg ctgctgaata gggtgattgt tgggccataa tatgtcgcca ttgttagcgg
    27481 gggaagcaaa atgactcccc cggttgtact cgctaaagag tacgatagca tatcataaac
    27541 gtgcggatca gtaacgacgt ccccattccg tggcgctcat caacgtcggt ttatccccca
    27601 tgaaatagcg gtagtaagca tcataagcca gcacgttctt cacataaccg cgcgtctcgg
    27661 agaacggaat actctcgaca aatgccacag cgtcgatacg cccggcgctg ttgccaagcc
    27721 aggttcgcac ccgtccaggt ccggcgttat aagctgctga ggagaaaata cggttattgc
    27781 caaactgctg ataaacatat tgcaggtaac tggtgccaat gttgatgttc gtttccggat
    27841 ccagcaattg cccagggctg ctataaccag gaatagagaa catcttcacc gtatgggtcg
    27901 ctgtaccagg cataatctgc atcaggccgc tggcccctac cggtgatttc actttcggat
    27961 tccaggcgct ctcctgacgg gcaatcgcca tcgcatagct ttgcgggatc tctttcccac
    28021 tggtgtaacg tttgaaaaga tcgttgtaag ccagcgggaa tcgctcttcc agatgatccc
    28081 aaagcttccc ggcgatcgtt gcctgaacgc taagatccca ccattggtta ttgaaagcat
    28141 accgagccag ttgagcctgc tctgtttttg acttgctctt caccagattg gcccactcgc
    28201 tacgcgcggt attatcgaga ttccagtaca tcaactcgcg cacgcgcgcc atctccggcc
    28261 cctgagtcag ggtgctgtca acattctgcg gcgctttatc aatcttcagc tcatactctt
    28321 caccgatgcg ttgtgctgca accatcgggt agaaaccacg ctgttgtatg agttgatgca
    28381 aaatctcttt cgcttcagct tcacgtccgc gttccagcaa taaatccgcc tgccagtaac
    28441 gccattcatc tttctctttt gcttccatcg gcagacgcgc cagccaggta ttcaggccgc
    28501 gacgatcgcc ggtgccaagc gccattcgca cacggcgttc aatcaacgac gtggattgcg
    28561 agcgcataat ggcgtcatcg cgccatttcg cctgctcatc ggtgacatca ttgcccatca
    28621 aacgccaggc gacgatatcg cgcagctcct gaatctgatc ttcattaagc tgctgcgcct
    28681 gggcaagcga cgggatcatc agccgtgcat tttcggcatc ctgccgcgcc acactggcaa
    28741 acgccaccgc cgccatttga cgggtaaaat cggtcgcgcc ggttgtacgc gcgaaggtca
    28801 gtaccgtatt agggttgttc gccagtgaaa tgattgccga ggcgatagtc tggtaatcgg
    28861 caggcatctg ccctgccagc actgttacca ggcctgtgtt acccgctttc atcgccagac
    28921 ggatacgctc taaatacgcc agcggatctt gtttacctga cgcacgccag acgctaaata
    28981 acttgtcaca ggcgttaggc tggctcttgc cggttagcca cagctctttc gccccttgcc
    29041 aggcttcttc actctgcccg gtgttccatt tcgcatagta gtaattacat tgcgcttcgg
    29101 tagttccggg cttttccggg ctaaaggcta acaagccacg ccagtcttca cgccgcgcca
    29161 gttcattgac gaaacgagat tgcagcgtgc gagcgggagg aagcgtgggg ttagcgcgaa
    29221 caaagttagt gaccgtcacc gccggttgat tcatcaggtc atcggtgatc tggcggtatt
    29281 ccaggtaggg ataaagcgga taatccttca gtccaggcat catttgttcg accacatcca
    29341 tctgtcgatt atcccaggcc tgcttgattt gcgcgtaacg gctacgctgc tcatccagtg
    29401 agtcggctcg cgccacgctg ctgaccgtca gcagacagac accggcagcc aacagccgcc
    29461 aggtaacttg tttggctttt tccacaagcg catcctctaa gtgtaaatac atcaatgcag
    29521 cgtcatgccg cgtaatagat ttatgctaac cagtcattgc cgtttacgcc acgttacgga
    29581 cactttttta cttttactgc gaaggtgatc gaccaactgg gattaggcgg ggaaacaggc
    29641 tacacttcgc ctttgaaaac tcatcatcac gataaacata gaggcgaagt ccaacgtggc
    29701 tcaattcgtt tataccatgc atcgtgtcgg caaagttgtt ccgccgaaac gtcatatttt
    29761 gaaaaacatc tctctgagtt tcttccctgg ggcaaaaatt ggtgtcctgg gtctgaacgg
    29821 cgcgggtaag tccaccctgc tgcgcattat ggcgggcatt gataaagaca tcgaaggtga
    29881 agcgcgtccg cagccagaca tcaagattgg ttatctgccg caggaaccgc agctgaaccc
    29941 ggaacacacc gtgcgtgagt ccattgaaga agcggtttca gaagtggtta acgccctgaa
    30001 acgcctggat gaagtgtatg cgctgtacgc cgatccggat gccgattttg acaagctggc
    30061 cgctgaacaa ggccgtctgg aagagatcat tcaggctcac gacggtcata acctgaacgt
    30121 acagctggag cgtgcggcgg atgcgctgcg tctgccagat tgggacgcga aaatcgctaa
    30181 cctctccggt ggtgagcgtc gtcgcgtggc gttgtgccgc ctgctgctgg aaaaaccaga
    30241 catgctgctg ctcgacgaac cgaccaacca cctggatgcc gaatccgtgg cgtggctgga
    30301 acgcttcctg cacgacttcg aaggcaccgt ggtggcgatt actcacgacc gttacttcct
    30361 cgataacgtt gcaggctgga tcctcgaact ggaccgcggt gaaggaattc cgtgggaagg
    30421 taactactcc tcctggctgg agcagaaaga tcagcgtctg gcgcaggaag cttcacaaga
    30481 agcggcgcgt cgtaagtcga tcgagaaaga gctggagtgg gtacgtcagg gaactaaagg
    30541 ccgccagtcg aaaggtaaag cacgtctggc acgctttgaa gagctgaaca gcaccgaata
    30601 ccagaaacgt aacgaaacca acgaactgtt tattccacct ggaccgcgtc tgggcgataa
    30661 agtgctggaa gtcagcaacc tgcgtaaatc ctacggcgat cgcctgctga ttgatgacct
    30721 gagcttctcg atcccgaaag gggcgatcgt cgggatcatc ggtccgaacg gcgcgggtaa
    30781 atcgaccctg ttccgtatga tctccggtca ggaacagccg gacagcggca ccatcacttt
    30841 gggtgaaacg gtgaaactgg catcggttga tcagttccgt gactcaatgg ataacagcaa
    30901 aaccgtttgg gaagaagttt ccggcgggct ggatattatg aagatcggca acaccgagat
    30961 gccaagccgc gcctacgttg gccgctttaa ctttaaaggg gttgatcagg gtaaacgtgt
    31021 tggcgaactt tccggtggtg agcgcggtcg tctgcatctg gcgaagctgc tgcaggttgg
    31081 cggcaacatg ctgctgctcg acgaaccaac caacgacctg gatatcgaaa ccctgcgcgc
    31141 gctggaaaac gccctgctgg agttcccggg ctgtgcgatg gttatctcgc acgaccgttg
    31201 gttcctcgac cgtatcgcca cgcacatcct ggactaccag gatgaaggta aagttgagtt
    31261 cttcgaaggt aactttactg agtacgaaga gtacaaaaaa cgcacgctgg gcgcagacgc
    31321 gctggagccg aagcgtatca agtacaagcg tattgcgaag taatgcgtaa aatgccggat
    31381 gcggcgcgaa cgccttatcc ggcctacaaa accagcaatt tcaatacgtt gtaaaactgt
    31441 aggcctgata agacgcggta agcgtcgcat caggcattga gcaccgaatg ccggatgcgg
    31501 cgtgaacacc ttttccggtc tacaacagcc ttagcatcct caatttcgtg gcgggtcacc
    31561 cgtgcaggtt ttttcaaacc ttgtttaatc tcgacagcct cttccagaga ggttttcaac
    31621 tcatcaaaaa aactcatttt atacctcgcc tttgagcagt ttcgtcacgg ttatccttgc
    31681 tcccccatca tctcccgcac cagctccacg cagcgcagga aacggctgtc gtaatcgtcc
    31741 tcttcaaccc gcacgaattc gatattgttc tcttccagca tctccaccag caagttctgg
    31801 aactctttgc gatccaccga actgccgagg ctgcgtaaac catccgccac ccacggcgtg
    31861 ttgttctcca gcaggatcac cagatcgaaa cggtattcat caatcagcgc ctgcacgaac
    31921 ggatgctcac gcccttcgta ctttttgcag aacgcctggg tagtgacaaa atcggtgtca
    31981 ataaacgcca ctttattggc gtatttcact gcaaaatcaa tgtattgtgc gtgacccaac
    32041 gcgattttat cgtagtcgga atactgcaat gcgatttcat caccgccgag gtgtgaaaag
    32101 acataatcgc ggccatattc ccacgcactg gtggtgttga agatattggc aagtttgttt
    32161 accagggtgg atttaccgct cgactcgccg ccaaggatcg ccacggtacg cacaaaaaac
    32221 ggcttcactt cggtaggaat atattcccag tagcggaacg ggttttcgcg gatctgcgca
    32281 ccgctgatac tcataaaggt acgtttcgga tcgaccagca ccgtctcgat ccccagatgt
    32341 tccatatact gtggcgcatc ggcttcttcc gaggtgtaga tcaggtccgg ctggatcccc
    32401 ttttcagcca taaacttttt gatgccgttg ctccacacat cccagccatg cggatacggc
    32461 tccatgccct cttcgttgaa agcatgaata cggatatttt tctgatattt aaaggtttgc
    32521 aatagccagc gcagacgatc cggcaccgtg ggctgctgcg acatggcgct gtcttcaaac
    32581 aacgcgcggt cgcgggtatc gtcaaaaccc ataatgatat gcagctcgtc aacctggcta
    32641 caggcgcgct ggataaggta gatatgtccg gtatgcagcg ggtagaactt accgaatacg
    32701 acaccaatgg ttttcttctg ccggggaaat tcaagcccca aaaaacggtg caatgcctcc
    32761 agcttttgcg cgctggggct tttaattttg gcattcagta actggcttaa ataccctttg
    32821 gtcataccgc tggcatcagc tacctgctgt agcgtgcagc cctgttgctt gatggcagtt
    32881 ttcaggtaat caaatgacga catatctccc tccgtatctc tcattataag tcgtcgaaca
    32941 cgctaagcgc gtcggagagt tttttaacgc caaaaatctg catcccttcc ggcgcttttt
    33001 tcggtacgtt agccgccgga acaatcgccc ggcgaaaacc gtgtttcgcc gcttcagaaa
    33061 tccgctcctg gccactgggc accgggcgga tctcccctgc cagcccgact tcaccaaaca
    33121 ccaccagatc ctgtggcagc ggtctgtcac gcaggctgga aaccatcgcc agcagtaacg
    33181 ctaagtcggc gctggtttcg gttaccttca cgccgccgac cacgttcaca aacacatcct
    33241 gatcggccat ttgcagacca ccgtgacggt gcaagacagc cagcaggatt gccagacggt
    33301 tttgttccag ccccactgcc acgcggcgcg gattcgccat tatcgagtga tcgaccagcg
    33361 cctgaatctc caccagcaac ggacgcgttc cttcccacac caccatcacg gagctgccgg
    33421 aggtcacttc atctccgcga cttaagaaaa ttgccgaagg gttgctgact tcacgcagcc
    33481 cctgttcggt catcgcgaag acgcccagct cattcaccgc gccgaagcgg tttttatggc
    33541 tgcgtaaggt gcgaaaacgg gagtcggcat cgccatccag aagcaccgaa cagtcgatac
    33601 agtgttccag cactttcggg ccagccagcg aaccatcttt ggttacgtgt ccaaccatga
    33661 caatcgccac accgcgcgtt ttggcgaaac gcgtcagata agccgccgtt tcacgcacct
    33721 gcgccacgct accaggcgat gattgtacat ccgccatatg catcacctgg atcgagtcaa
    33781 ttaccatcag cttcggttgc tcttcttcgg caatcaggca gatctgctcg atgctggttt
    33841 ccgacaacat attgagattg tcagtcggca ggccaaggcg atgagcgcgc attgccacct
    33901 gttgcagcga ctcttcgccg gtgacataca gcgttttcat ctgctgggcc agtttgcaca
    33961 gcgtttgcag caacagcgtg gatttccccg caccagggtt accgccaatc agaatggcac
    34021 ttcctggcac cacgccgcct cctagtacgc ggtcgaactc tttaaatccg gtggaaaaac
    34081 gcggcagctc ttcaaggctg atatcggaga gtttctggac ttttgccacc ccggcgctac
    34141 cggcatagcc gctgagacgc tcgttacgcg ccaccgttgg cgacgcagca agacgcacct
    34201 cggtgatggt gttccaggca tgacaggcac tgcactgccc ctgccagcgc ggataatcgg
    34261 ccccgcattc attacaaaca aaggcgcgtt ttggagcttt tgccacggtg tacctcgtta
    34321 atgctgtgcc gcccgcagga tgacgggcag gcaattactt ctgattcagg ctgccagaga
    34381 ggatgcagaa tacccccatc aggtcagcgt gacggatggt gacttccgcc ttttcattca
    34441 cttttggctt ggcatggtag gcaatcccca gccctgctgc tttgatcatc ggcaggtcat
    34501 tagctccatc gccaatcgcc acggtctgcg ccagcgggat ttcatactcc tgcgcgaggc
    34561 gagtcagagt tttcgctttg tactgcgcgt ctacgatgtc gccgatcaca ttgccggtaa
    34621 atttaccgtc catgatctcc agttcattgg ctaccacggc tgtcaggcgc agcttgtcgc
    34681 gcaggtattc agcaaagaaa gtaaagccgc cggaggcaat cgccactttc cagcccagcg
    34741 tttccagctt gagcaccagc tgcgttaagc ctggcatcag cggcagattt tcacgcacct
    34801 gttgcagaat attggcgtca gcgcctttca gcgtcgccac gcggctgcgc aggctggcgg
    34861 taaaatcgag ttcgccgcgc atcgcccgtt cggttacctc cgccaccatc tcgcccgttc
    34921 cggccagctt ggcaatttca tcaatacatt caatctggat ggcggtggaa tccatgtcca
    34981 tcaccagcaa acccggcgtg cgcaggtgcg ggattttccc cagcggggcg acatccagct
    35041 gcgcttcgtg ggccaggcgt gtagcccgtg cggtgagtga acctgccaga cgaatcacct
    35101 gataatcttc cacgcaccag gcggcaacaa tcaccatcgc cgcacccagt ttgctctggt
    35161 attgggtcag acgctgttta tccagcccac gaccatacag cagccagccg ctacgacctg
    35221 cgtggtaatc cagtggcatc acttcatcac cacttaatga aagaggcaga cccggccata
    35281 aagagacatc ttcaggcagg tcgcaccagg taatgttagg cattaaggct cctgtaaaat
    35341 cgttcgaagc agggaaaata acgcatgagg ctaccttgta tccattgctt ctggcaacat
    35401 taagtctcaa attttcaaag ggtggaagat ggctcgcaca aaactgaaat tccggctaca
    35461 tcgggcagtg attgtcctgt tctgtcttgc cttgttagtg gcactgatgc agggggcgtc
    35521 atggtttagt caaaaccacc agcgacagcg taatccacag ctggaagaac tggcccgcac
    35581 cctggcgcgt caggtgacgc tgaacgttgc accgctgatg cgtaccgact caccggatga
    35641 aaaacgcatt caggcgatcc tcgatcagtt aacagatgaa agccgtatcc tcgacgcggg
    35701 tgtgtatgac gaacaaggcg atcttatcgc acgttctggc gaaagcgtcg aagtgcgcga
    35761 tcggctggcg ctcgacggta aaaaagcagg cggctatttt aaccagcaga ttgtcgagcc
    35821 aattgcgggt aaaaacggac cgctcggcta tctgcgcctg acactcgaca cccatacgct
    35881 cgccaccgaa gcccaacagg tggataacac cactaacatt ttacgcctga tgttgctgct
    35941 ctcactggca atcggcgtag tgctgacccg cacgctgcta cagggtaaac gcacccgctg
    36001 gcagcaatcg cccttcctgt taaccgccag caaaccggtg ccggaagagg aagaaagcga
    36061 gaaaaaagag tgacccatta ctacaagaaa ggaaatcgtt atgtccacat tacgcctgct
    36121 catctctgac tcttacgacc cgtggtttaa cctggcggtg gaagagtgta tttttcgcca
    36181 aatgcccgcc acgcagcgcg ttctgtttct ctggcgcaat gccgacacgg tagtaattgg
    36241 tcgcgcgcag aacccgtgga aagagtgtaa tacccggcgg atggaagaag ataacgtccg
    36301 cctggcgcgg cgcagtggcg gtggcggcgc ggtgttccac gatctcggca atacctgctt
    36361 tacctttatg gctggcaagc cggagtacga taaaactatc tccacgtcga ttgtgctcaa
    36421 tgcgctgaac gcgctcggcg tcagcgccga agcgtccgga cgtaacgatc tggtggtgaa
    36481 aaccgtcgaa ggcgaccgca aagtctcagg ctcggcctat cgcgaaacca aagatcgcgg
    36541 cttccaccac ggcaccttgc tactcaatgc cgacctcagc cgcctggcaa actatctcaa
    36601 tccggataaa aagaaactgg cggcgaaagg cattacttca gtgcgttccc gcgtgaccaa
    36661 cctcaccgag ctgctgccgg ggctcaccat gagcaggttt gcgaggccat aaccgaggcc
    36721 tttttcgccc attatggcga gcacgtggaa gcggaaatca tctccccgga caaaacgcca
    36781 gacttgccaa acttcgccga aacctttgcc cgtcagagta gctgagagtg gaacttcggt
    36841 caggctccgg cattctcgca tctgctggat gaacgcttta gctggggcgg cgtggaactg
    36901 catttcgacg ttgaaaaagg ccatatcacc cgcgcccagg tatttaccga cagcctcaac
    36961 cccgcgccgc tggaagccct cgccgggcga ctgcaaggct gcctgtaccg cgcggatatg
    37021 ctgcaacaag agtgcgaagc gctgttggtt gacttcccgg accaggaaaa agagctacgg
    37081 gagttgtcga cgtggatagc gggggcggta aggtaactcg ctgattaaaa tatccaagga
    37141 aggaaatatg atcgatctgg aaaaccagga acgggagatt atcaatttaa tgttcagcca
    37201 gcgtatttcc tggctagccg ccgtgcgtat caggcataaa ttatcgcttg ctgaagtaag
    37261 taaaatgtta ggtatctcga ttaattctct aaaacagatc gaaaagacgg aacggcttag
    37321 ctcaaacatc aaaagcaaaa tggcagaaat ttatggttgt ccaccagagt tgctgatatg
    37381 cccttcctgg atgacggctg aacataaatg aatagattcg tgaatagata cttgaatgtt
    37441 ttttattttc tatccatatg aaaaataata gtaaatctat aaatcagttg ctattgatga
    37501 atagatacta aaaagccgga gcagtccccc actccggctt aacacgacaa tcgcctttca
    37561 gcgaaacaca attactcttt atcgcccagc agaacggatt ccagtgcgat tttgatcatg
    37621 tcgttgaagg tggtctgacg ctcagcggca gtggtctgct cgtgagtgcg gatgtggtca
    37681 gacacggtgc agatggtcag ggctttcgcg ccaaactctg cagccacgcc gtagatacca
    37741 gccgcttcca tttccacgcc gaggatgccg tatttttcca tcacgtcgaa catttcgccg
    37801 tccggagagt agaacaggtc agcggagaac aggttaccca cgcgagcatc aacgcccagt
    37861 gctttagctg catctactgc gttacgcacc atgtcaaagt cagcgatagc ggcaaagtca
    37921 tggtctttaa aacggatgcg gttaactttg gaatcggtgc aggcccccat accgataacg
    37981 acgtcgcgca gttttacgtg cggcagaact gcgccacagg aaccaacgcg gataattttc
    38041 ttcacgccga aatcggtgat cagttctttg gtgtagatgg agcaggacgg gatacccata
    38101 ccgtgaccca ttacggaaat tttgcggcct ttgtaagtac cagtgaagcc cagcataccg
    38161 cgaacgttgt tcacttcacg ggcatcttca aggaaagttt cagcaatata cttcgcacgc
    38221 agcgggtcgc ctggcatcaa aactacgtca gcgaaatcgc ccatttctgc attaatgtgt
    38281 ggggtagcca ttgttttatc ctttttgtga cataacaaag gcggagtcaa cgctccgccc
    38341 aaatccatca gaacatggct ttgccatatt ccatatcaga agtaccaaaa tattttgcca
    38401 gagtctggcc gatatccgcg aaggtttcac ggtgacccag tgagcccggt tttactttcg
    38461 ggccatatac cagtaccgga atgtgttcac gcgtgtggtc agtaccggtc caggtcggat
    38521 cgcaaccgtg gtcagcggtg aggatcagga tgtcgtcatc gcgcagcaga gacatcagct
    38581 ccggcagacg gcggtcgaac agttccagac ccgcagcata accggcgacg tcgcggcggt
    38641 ggccccagga agagtcgaag tcaacgaagt tggtgaagac gatggtgtta tcacccgctt
    38701 ctttcatctc tttgatggtg gcgtcaaaca gcgcgtccaa gccagtcgct ttcacttttt
    38761 tggtgatacc gcagttggcg tagatgtctg caattttacc gacagaaacc acctggccgt
    38821 gtttttcatc aaccagtttc tgcagcacgg tcggtgctgg cggctcaaca gccaagtcgt
    38881 gacggttacc ggtacgctgg aagttaccag ctttgtcacc gataaacgga cgagcgataa
    38941 cacgaccgat attgtagccg ccgttggtca gctcttcacg ggcgatttcg cacagttcgt
    39001 agagtttatc cagaccgaaa gtttcttcat ggcaggcaat ctggaacacg gagtcagcgg
    39061 aggtatagaa aatcggcttg ccggttttca tgtgctcttc gcccagttga tccagaatga
    39121 ccgtaccgga agagtggcag ttaccgaggt aacccggcag attagcgcgt tcgaccagtt
    39181 tatccagcag ctcttgcggg aagctgtttt cgtgatcgga gaaatatccc cactcaaaca
    39241 gaaccgggac accggcgatt tcccagtgac cagacggggt atctttaccg gatgacattt
    39301 cgtgcgccca tgcgtacgcg ccgataactt cagcgttgcc gtccattccc gccggaatga
    39361 aaccggtaga accttcgtgt gctttcgcca gccccagacg ggtcagattt ggcagattga
    39421 gcggcccttt acgaccatta tcagcttcgc ctttggcaca agcttctgcg atatgaccca
    39481 gggtgtcagc cccgacgtca ccaaagcgtt ctgcatcttc tgtagcgccg atgccgaatg
    39541 agtccagcac cataataaat gcacgtttca tatgttctcc gtactcagtg cttcaaattt
    39601 aaatgatcag atcagtatac cgttattcgc tgatacggcg atagacagtt ggtgtgcttt
    39661 ccggtgcgtt atcggcaagt ttaattgccg ctttcaccgc tttcgccgct tcctgccagc
    39721 tgttttcgtc tttcgcgagg ataaccgcca gcggacgctg accgtctacc tggtcgccca
    39781 gacgcgccat atcagtaaag ccgacgctgt aatcgatggt gtcagatgcc tgacgacgac
    39841 cgccgcccat tgcaactact gccatcccca gcgcgcgggt atccatttca ctgacaaaac
    39901 cttcggtatc agcatagact gctttcgtca gcatcgctgt cggcagatac ttcgcgtagt
    39961 tctcaacgaa gtcggtcggg cctttttgtg ccgctaccat acgaccaaag acttctgccg
    40021 ctttaccgtt gtccagcacc gcctgcaatt tcgcgcgcgc ttcggcgtca tctttcgcca
    40081 gtttgccgga gatcaacatc tccacgcaca gcgccatcgt gacatcaaac agacgcgggt
    40141 tacgatactc acccgtcagg aactgcaccg cttcacgaac ttcaaccgcg ttacctgcac
    40201 tggaggccag tacctgattc atgtcggtga gcagcgcggt ggtgcgcacg ccagcgccgt
    40261 tagccacgcc aacaatcgct tcggcaaggg cttcagagag ttcgtaggtc ggcataaacg
    40321 cgccgctacc cactttcacg tccatcacca gcgcgtccag accttccgcc agtttcttcg
    40381 ccaggatcga ggcagtgatc agcgggatgg agtccacggt tgcggtaata tcacgggtcg
    40441 cgtagaaacg tttatcagcc ggagccagtg aactggtctg accgataatc gccacgccga
    40501 cgtctttaat aatttcgcgg aaacggttgt catccgggaa aatgtcgaag ccagggatgg
    40561 attccagttt gtcgagcgta ccgccagtat gaccgaggcc gcgaccggag atcatcggaa
    40621 tatagccgcc gcaggctgcg accatcggcc ccaacatcag cgaagtgaca tcgccgacgc
    40681 cgccggtcga gtgtttatca acaatcgggc cattcagatg caggcttttc cagtcgagaa
    40741 cggttcctga atctcgcatc gccatggtca gcgagacacg ctcaggcatt gtcatatcgt
    40801 ggaagaaaat ggtcatcgcg agggcggcaa tctgcccttc ggaaatagtg ttgtcgcgaa
    40861 taccgttgat aaagaaacga atttcttcat cgctcagcgc atgaccatca cgttttttac
    40921 gaataatttc ttgtgcgaga aacaaggtaa cctcccgagg aaaagagtat gaggcggctt
    40981 ttgccggatg cgtctacgcc ttatccggcc tacgggtaga tacgtctcgt aggcctgata
    41041 agcgcagcgc atcaggcgta aagcatctta cttagtagct gctggcgctc ttaccgtcgc
    41101 cgtggcccag cgctttcagc aggcttgcca gcaggctgga agcaccaaag cggtagtgac
    41161 gcgcatcagc ccagtcagca ccgaacagtt catctgcgat ggcgagatat ttctgcgcat
    41221 cttccgcagt acgcacgccg cccgccggtt tgaaaccaac ggttttttct acgcccatat
    41281 cacggatcac ttccatcatg atgcgagcgc tttccggcgt cgcgttcaca gccactttac
    41341 cggtagaggt tttgatgaag tccgcacccg ctttgatgga gatttcagac gctttacgga
    41401 tcagcgcttc gtctttcagt tcgccggttt cgatgatcac tttcagcagt acattcgctg
    41461 ccgcgcaagc ctctttacag gctttcacca ggtcaaaacc aacctgctcg tcacccgcca
    41521 tcagcgcgcg gtacgggaac accacgtcaa cttcatcagc accgtaggcg attgccgcac
    41581 gggtttctgc cagcgcgatt tcgatgtcgt cgttaccgtg tgggaagttg gttaccgtag
    41641 cgatacggat ttccggggtg ccctgctctt tcagagtttt gcgagcaatc gggataaaac
    41701 gaggatagat acagatagcg gcggtattgc cgaccggagt tttggcctga tgacacagag
    41761 caattacttt ctcgtcggtg tcgtcgtcat tcagggtggt caggtccatc aatttcagtg
    41821 cacgcaggct gcttgctttc agatcagtca tttcattctc ctggcttgtc gccaataaaa
    41881 ttcaccttgc gagtttgtta gtattctaac atctactccg caacacactt cgatacacat
    41941 cacaattaag gaaatctact tacaagtttg catcactgta atgcgatctg gttcaaataa
    42001 ttcactttca aatgaatgcg tcagtggtgg caaacgcatc aggatcaaag tgaacatcac
    42061 gaaacttctt acaatggcgc atcaccttcg gcataaaagg aaaagaagat gcccacttct
    42121 catgaaaatg cactgcaaca acgttgccag caaattgtca ccagcccagt gcttagcccg
    42181 gagcagaagc gccattttct ggcactggaa gcagaaaaca atctgcctta cccacagctt
    42241 cctgccgaag cccgccgcgc gctggatgaa ggtgtaatct gcgatatgtt tgaaggtcat
    42301 gcgccgtaca aaccgcgcta tgtcttaccc gattacgctc gttttctggc gaacggttcc
    42361 gaatggctgg agctggaagg cgcgaaagat cttgatgacg cactctctct gctgaccatt
    42421 ctttaccacc acgtaccgtc ggtcacatcg atgccggtct acctggggca actggatgcg
    42481 ttgttgcaac cgtatgttag aattctaaca caaaacgaga tcgatgttcg aataaaacgt
    42541 ttctggcgtt acctcgacag aaccctgcca gatgccttta tgcacgccaa tatcggcccg
    42601 tctgattcgc ccattacccg cgcgatctta cgtgcagacg cgcaggtttc accgaacctg
    42661 acctttatct acgatcctga aatcacccct gatgacctgc tgctggaagt ggcgaagaac
    42721 atctgtgaat gtagcaaacc gcacatcgcc aacggtccgg tgcatgataa aattttcaca
    42781 aaagggggct acgggattgt gagctgttac aactcactgc cgctggcggg tggtggcagc
    42841 acgctggtac gccttaacct gaaagccatt gccgagcgca gcgaatcgct ggatgacttc
    42901 tttacgcgca ctctaccgca ctactgccag cagcagatcg ccatcatcga tgcgcggtgt
    42961 gaattcctct atcaacaatc acacttcttt gagaatagct tcctggtgaa agaagggctg
    43021 attaaccctg aacgttttgt gccaatgttt ggcatgtatg ggctggcgga agcggttaac
    43081 ttgctgtgtg agaaagaagg gattgccgcg cgctacggta aagaagccgc cgcaaatgaa
    43141 gtgggttatc gcatcagcgc gcaactggcg gagtttgtcg ccaatacccc cgtgaaatat
    43201 ggctggcaaa aacgcgccat gttacacgca cagtcgggga tcagttccga tatcggcacc
    43261 acgccgggcg cgcgtttgcc gtatggcgat gagccagatc cgatcaccca tctgcaaact
    43321 gtcgccccgc atcatgctta ttattattcc ggcatcagcg acattctgac gctcgacgaa
    43381 accatcaaac gtaacccgca ggcactggta cagctttgcc tcggtgcctt taaagccgga
    43441 atgcgtgaat ttaccgccaa tgtcagcggt aacgatctgg ttcgcgttac cggttatatg
    43501 gtgcgtttgt cggatttaga aaaatatcgc gccgaaggtt cacgcaccaa caccacctgg
    43561 ctgggcgaag aagccgcacg caacactcgt attctggaac gccagccgcg cgtgataagc
    43621 catgaacagc agatgcgctt tagtcagtaa gattatcccc ttctcctgcg ttgacgggcc
    43681 aggcagtcgt ctggctctgt ttttgcaggg ctgcaatctg cgctgcaaaa actgtcacaa
    43741 tccgtggacg atgggacgtt gcaatgactg tggggagtgc gtgccacagt gtccgcatca
    43801 ggcgttgcag attgttgacg gcaaagtggt gtggaacgct gtggtctgcg agcaatgtga
    43861 tacctgcctg aagatgtgtc cgcaacatgc cacaccaatg gcgcaatcca tgagcgtgga
    43921 cgaagtgctt agccatgtgc gcaaagcggt gctgtttatt gaagggataa cggtaagcgg
    43981 cggcgaagcc acgacccaac tgccgtttgt agtggcgctg tttactgcta tcaaaaacga
    44041 tccgcaactg cgccatctca cctgtctggt ggacagtaac ggtatgttga gcgaaaccgg
    44101 ctgggaaaaa ttactcccgg tgtgtgacgg cgcaatgctc gatctcaaag catggggtag
    44161 cgaatgtcat caacaattaa ccggacgcga taatcaacag attaagcgca gcatctattt
    44221 gctggcagag cgcggcaagc tggcggaact gcgtttgctg atgatcccag gccaggtgga
    44281 ttatttgcaa cacatcgaag aactggcggc gtttatcaag ggacttggtg atgttccggt
    44341 tcgcctgaac gcgtttcatg cccacggcgt gtatggcgag gcgcaaagct gggcgagcgc
    44401 cacgccggaa gacgttgagc cgttggctga tgcattaaag gtgcgcgggg tgagccggtt
    44461 gatatttccg gcgctctatt tgtgacatgc cggatacggc ctacggttcg gcacagactt
    44521 gtaggcctga taagacgcgt caagcgtcgc atcaggcatt gtgcgccaac tgccggatgc
    44581 ggcgtgaacg ccttatccgg cctacggttc ggcacaaacc tgtaggcatg ataagacgcg
    44641 ttaagcgtcg catcaggcat tatgcgccga ctgccggatg ctgcctacga agggcaaact
    44701 acccacgaac gttaaacacc gcatacgtgt tattaagcaa cacttcggca atctcatccg
    44761 cgggttccgg gcgtaactcg caaagcacgg caaacacacg ggcagcctgt tccgggcggt
    44821 taggctgccc ctgaaaaccg ttgagcggca tatccggcgc atcggtttcc agcaacaacg
    44881 atgccagcgg taattttgcg atgacatcgc gggttttact ggcgcgtgga taggtgatag
    44941 taccgcctac gccgattttg tagcccagct gtacaaaccg ttcagcctgt tgctggctgc
    45001 cagaaaatcc gtggacaaca ccagcacgcg gcaaatcgtg gcgtttaaga tgcatcgcca
    45061 gtttgtcgtg cgtgcgccgt gaatgcagga tcaccggcag atcgtagcgt ttcgccagtt
    45121 tcagttgttc gtcgagtaac cactgctgcc tctcaaattg cggatcgtcg ccgaagagat
    45181 ccagaccgat ctcccccacc gccaccacct tcgccggacg cctttccagc gcctgctgta
    45241 gctgctcaag agacacatcg ctatgttttt ccaacatacc aggatgcaag cccaatacgg
    45301 catacagcgg ttgataattt tccgctaacg cctgcacacg ggcaaaattt tccgcctctg
    45361 tcgccggaac aatgatcttg cctacgcccg cttgtgccgc gcgttgcagg ctggcctctt
    45421 catcgccaat aaaaggcggg aaatcaaaat ggcagtgagt gtcgataaaa cggcaaatca
    45481 agccagatcc tcattgttaa atgtcgggtc gttcgcctgc ggagcgtcgc tcacttcggc
    45541 aaccagcgta tcgttggcga ctggcgcagg cggaatgaca atcgattccg gcgtcaccac
    45601 tggcaccaga tggcgggtaa gcggcgcttt ttcagttaat agcttgccaa ccgtggcgag
    45661 gaaataacga ccgcataaac gcccaagttt atagtcttca cgcagcgccg gaatccgact
    45721 gccaagcgcg atactatgta atggcttcgg cggataaatt tcggatatcc gcagcttgcc
    45781 cggtggtttc tcaataaatt gctgaatgtc acgatagctg gtttcatgat gctgcaccag
    45841 attgaccagc ggctgcaggc tactgtcacc cagccagcgt tccatgcgtt tgaaccactg
    45901 cggcgtgtag tacatttgtg acggcacagt gcggatgacg accaacgttt tagcgccctg
    45961 ccttgccgcc tctttaaccg gaatcgcatc actgatcccg ccatccaggt agttaatgcc
    46021 ttccagcgac actccgctac gataaaagcc aggtatcgca ctggaggcgc gaatcacatc
    46081 cagccagttt tgtttggttg gtaaaaagta attcggcgcg tagtcatcct gacgacaggc
    46141 gcacatataa aacgatttgc cgctgtcaaa caaccgcgcg gcggtatcca tttgcaacgg
    46201 catctggctt gcggtggcct ccaccagcca gtcgagatcg ataagatttc ctccacggac
    46261 aaagcgcaat ggatcgaaaa attcgcgttt tgtggtatag cgcatgatga ctttgcgcgc
    46321 gtaaccgggc tgattgcaga taaacgccga gaggttctgc gccccggcag atgtgccgag
    46381 ataaagatcg aaaggattaa actgcgcgcg cataaactca tccagcacgc cagccgtgaa
    46441 aattccacgc tgtccgccgc cttcgcacac cagagctatt cgtccaggct ggaacggcct
    46501 tagcgacaac ggcgcaatat taccaagcgt tacaggtatt cgctgcccca cttctgcctt
    46561 cctgtttttg ttgtgaaaga aagacaaagt aacgcaattt attattcgct aaaaccgcaa
    46621 aagccagtcc gctggactgg ctttggataa aattttctta atggtctgaa atctagggtc
    46681 gttttcggcc catgaacaaa ctcactagga acagaataat cccgacgaca aagacaattt
    46741 tagctgcgcc tgcagcggta ccggccagac caccaaaccc aagtgcggcg gcgattaacg
    46801 cgataaccag aaatatgatg ccccaacgaa acataagcgt ctcctttacc atagttaatg
    46861 tcaccgcttg atatgagcgt gaaaatcact caatgcgata tttttagtgt ggtgcacatc
    46921 gcgcctcccg acaaagttcg ggaggacgaa ttacttagtt ttcagatcat ttttaacgct
    46981 tttcacacca tctaccgctt tggcgatact ttcagcacgg tcactttgtg cctgagaatc
    47041 tacggtaccg gagagctgaa ccacgccatc ggtggtttca actttcactt tacgtgaagg
    47101 gacgatatcg tccgccagca gtttggcttt gatttcactg gtggtggcgg tgtcacccgc
    47161 gtagcccttc accgagcctt ctttagcgtc gcgaacgtgc agtttgtcgc tgacagaggt
    47221 cacgccttca acgcctttcg ccactttcac tgcctcttcg gcctgggcct gactttcaac
    47281 gaaaccgctc agggtcacga ctttttgatc ggttttgaca gagatatcgg tgctcttgat
    47341 gttgtcatga tccaccagcg ccgccttcac tttcgcggtg atggcgctgt catccatgaa
    47401 attaccgact ttattcatag agctatcgac tttttgccct gcgctttcat tggtagtctg
    47461 cgcgttgttt tccgcgtagg cagagccggt cgcgacggca gaggtcaaca ttacagccag
    47521 cagagttttc gaaatcttca gtcttgtcat agtcatcgat ttattcctgt atgtttgctc
    47581 gtaatttgag cctagcaaca cgaggttgca ttgctgaata gggagagact tcaccctcta
    47641 cagaagtcaa tggtcgccat cacaaaagcg atgggtgatg aataacgacc attacagcct
    47701 ctgaatcagt tattaatatc ggtagaatga caatcgacgg ctttagatac taatatctac
    47761 gcattgaacg gtatttaacg ccatcagaaa tgtcatcact ttgttaaata tagatcacaa
    47821 ttttgaaacc gctcgggata tcagcgaaaa cataagcaaa agtgaatgtt ttaagaacat
    47881 tccgtaagcg gctaataagg aagggaaatt gacagggcgc agcggctacc gcgccctgga
    47941 ggcaagaatt aatgctcgcg ggtctggtgg aactgaacgt ccggataacg ttcctgtgcc
    48001 aggcgcaggt tgaccatgct ggtagcgatg taagcgaggt tatcgccgcc atcaagcgcc
    48061 agttggcttt cgttcttacg cttgaactct tcgaatttct tcgcgtctgc acattctacc
    48121 cagcgggcag tggcaacgtt gactgactca tacactgctt caacgttgta ttcgctcttc
    48181 aggcgcgcta ccaccacatc aaactgcagc acaccaaccg caccaacgat cagatcgttg
    48241 ttggagattg gacggaacac ctgcaccgcg ccctcttcgg aaagctgtac cagccctttg
    48301 agcagctgtt tttgcttcag cggatctttc aggcggatac gacggaacag ttctggtgcg
    48361 aagttcggaa taccggtgaa cttcatcatc tcaccctggg taaaggtgtc gccgatctga
    48421 atggtgccgt ggttgtgcag gccgaggata tcgcccggat acgcttcttc aacgtgcgaa
    48481 cggtcacccg ccataaaggt cagcgcgtcg gagatcacca catctttcgc agtgcgcacc
    48541 tggcgcagtt tcatgccttt ttcatattta ccggacacca cacgcataaa cgccacgcgg
    48601 tcgcggtgtt tcggatccat gttggcctga attttaaata cgaagccggt aaatttatct
    48661 tcgctcgctt ctacggtacg ggtatcggtc tgacgcggca tcggcgcagg cgcccactcc
    48721 accaggccat tcagcatatg atcaacgccg aagttaccca gcgcagtacc gaagaatacc
    48781 ggagtgattt cgcccgcaag gaacagctct ttgtcgaact cgttagacgc gcctttaacc
    48841 agttccagtt cgtcacgcag ctgctgtgcc agatcttcac caaccgcagc atcgagatcc
    48901 gggttattca gccctttaac aatgcggact tcctgaatgg tgtgcccttt accgctctga
    48961 tagagatagg tttcatcttt ataaaggtgg taaacgcctt taaacagctt gccgcagcca
    49021 attggccaag tgatcggtgc gcagccaatt ttcagctcgt tctcaacttc atcgagcagt
    49081 tccatcgggt cgcggatatc acggtcaagt ttgttcataa aggtgaggat cggcgtgtcg
    49141 cgcagacggg taacttccat cagcttacgg gtacgatctt caacaccttt tgcggcgtcg
    49201 ataaccatca ggcagcagtc caccgccgtc agggtacgat aggtatcttc cgagaagtct
    49261 tcgtgccccg gggtgtcgag caggttaacc aggcaatcgt gatacggaaa ctgcatcaca
    49321 gacgtagtaa tggagatccc acgctgcttt tccatctcca tccagtccga cttagcgtgc
    49381 tggttggaac cacggccttt tactgtaccg gcggtctgaa tggcctgtcc gaacagcagc
    49441 accttctcgg tgatggtagt tttaccggcg tccgggtgag aaataatggc aaaagtgcgg
    49501 cgcttcgcca cctctttcaa ataaggagac aacgtcataa ttctttcttc ttgagtaagc
    49561 ggcatcgcgc cgcgcttgtt gaaaacgaaa aattgcggct attttaccca tcaacggggg
    49621 ggaggcaatc agtgtttaca caggagctgc tccagttcgt gcaacgaaga aacggtccag
    49681 gtgggcgcga tgccttctgg ttgctcgcga ttgtgcgcat tcagccagca ggtcgcaagc
    49741 ccggcgttga tgccaccgag aatatcggac tcggcagtgt cgccaaccat cagcacgcgt
    49801 gaacggtcag gattgcccgc ctgttccagc gcataatcga aaattttctt attcggtttg
    49861 gcaacgccaa cttcttcgga aatcaccagc aaatcgaagt aatcacgcag gcccgtccgt
    49921 tccagacgca cttgctgcaa ggcgctaaaa ccgttggtga tgatgccgat tttagcgttg
    49981 ctacgaatgg cgttaagcag agaaaccgcg cccggcaacg gcgtacagat ttccgccatc
    50041 gcattaataa aggcatcgtt aagcaagcct gcttcaactt tcagccgttc ggcccagctc
    50101 tcgaaacgcc cgtgctgaag ctgtaatgaa gtgatcgcgc cgttttgata atccacccac
    50161 agtggcttgt taacggcctg atagtcctga aaatcttcag cggtaaaggt gacgctgtaa
    50221 tcaagaaaca tccgctgcag gccggtgaat gagtcaaagg taaacagcgt ttcatcggca
    50281 tcaaagaaaa tccagtccca cttcattatt ccaccttgtc ttacatactg attggcaacg
    50341 ccatgatgat ggcgtcttcg cgaccgtccg tggtggggta gtaattgcgg cgaatcgtcg
    50401 cctcgttaaa gcctaaactt tcgtacaggg caatggcggc agcatttgag gcacggactt
    50461 ccagccatag tgtcgccacg ccgcgttttt ccagttcgtc gatcagatgt tccagcagcg
    50521 ccctacccag cccctgacgc tgatagtcgg gatcgaccgc aatattgaac aatgtagctt
    50581 catccagcac cacttgcgta atcgcaaacg ccgccatttt gccgttttgc gttaactgaa
    50641 agttgagata acgctcgccc tggttgctgg caaaggtttt ttcactccac ggaaaggcgt
    50701 gggcgcgttg ttcaatgtgg taagccgctg gtaaatcagt cgtttcgagg gaagaaatcg
    50761 tgttcatatg tgcaaatttg ttgccataac gcggcgcgtg ccgttgggtt tgcccgtaat
    50821 tcggtgagcg ccggtgatgc cacctgagcg ccttccaatg atagcggttc gtcagtaccc
    50881 aaccgccagc tgttgcagcg actgccttgc ggcagcatcg cgattttttc tggcgtcagt
    50941 tgcagcactt gatcggggct gacggttaat gcgcgcagaa catcgctcac taaaggatca
    51001 gtcagggcgg gaagatcgtt tgccaccatc accagacgga cgtgtgccgg gctcgcaatg
    51061 gcaatctccc cctgcaacgc gccagggcga cgcagcgacc actgggtaat gcccagttgc
    51121 tgtaactgcc agtctcgtcg ggatgtcata gcgaatcgct cctgttgtca ggggcgcaaa
    51181 tatagcaaat tcgtcgatac cgcgccaaca tatggctata atcgccgcca gtatcaattg
    51241 aggagcattc catgtctgca tttaccccgg caagtgaagt cttgctgcgt cacagtgatg
    51301 atttcgaaca aagccgtatt ctgtttgccg gagacttaca ggatgacctg cccgcgcgtt
    51361 tagataccgc ggccagccgt gctcataccc agcaattcca ccattggcag gtgttaagct
    51421 gccagatggg ggataacgcc cgttttagtc tggtcgccac ggcgaatgat gtcgcagatt
    51481 gcgatacgct gatttactac tggccgaaga acaaaccgga agcccagttc cagttgatga
    51541 atttactttc tctgctgcca gtggggacag atatttttgt cgttggcgag aaccgcagcg
    51601 gcgtgcgcag cgctgagcag atgctggcag attatgcgcc gttgaataaa gtcgacagcg
    51661 ctcgtcgctg tggcctctat tttggtcgtc tggaaaaaca gccagtattt gatgcggata
    51721 aattctgggg cgaatacagc gtcgatggcc tgacggtcaa aacgctgcct ggcgtgttta
    51781 gccgcgacgg tctggatgtc ggtagccagt tgctgctctc gacgttaact ccgcacacga
    51841 aaggtaaagt gctggatgtc ggctgtggcg cgggtgtact ttcagttgcc tttgcgcgcc
    51901 attcgccgaa aattcgtctc accttgtgcg atgtctctgc cccggcggta gaagccagcc
    51961 gcgcaacact tgcagccaac ggtgttgaag gtgaagtctt tgccagcaac gtcttttccg
    52021 aggtgaaagg ttgttttgat atgatcatct ccaacccgcc gttccacgat gggatgcaaa
    52081 ccagcctgga tgcggcgcaa acgctgattc gtggcgcggt gcgtcatctt aatagcggcg
    52141 gcgagctgcg aattgtagcg aacgccttcc tgccttaccc ggacgtgctg gatgagacat
    52201 ttggcttcca tgaagttatc gcgcaaaccg ggcgcttcaa ggtatatcgc gccattatga
    52261 cccgccaggc gaagaaaggt tgagtatctg atatctgtcg ccgcataccg tgtcggatgc
    52321 ggcgtgaacg ccttatccga cctacaaaac ctgcacgtta gcccttcgta ggccagataa
    52381 gacgcgccag cgtcgcatct ggcatctgcc cccgcatgcc taccaaacct gcacgctagt
    52441 ttcctgatgg acatttttcc agcaattaca catcaggcga taattaacta ttgacgaaaa
    52501 gctgaaaacc actagaatgc gcctccgtgg tagcaattct ttttaagaat tgatggtatg
    52561 cgaaggtggc ggaattggta gacgcgctag cttcaggtgt tagtgtcctt acggacgtgg
    52621 gggttcaagt cccccccctc gcaccaatta tctttacttc ctttcttgtt tcttccctgt
    52681 tttgtcatcc ctgattcaaa ttcattatca tcagcatgat gccagcaata agcgccaggc
    52741 acgatggtag caaaatgtaa tgccgcaact gtttgtgata cagcatcacc ggcgtcaata
    52801 ctaatcccat cagaatgaca atccgccact gatccggcga taaatccgca tacatcagcc
    52861 ctgcaatcag caatcccggc agcaacactc cccagccact gcccagcgta cgttgcaaca
    52921 tggtttcatc tctttcattg ataatgaaaa ccaatatcat atgataattt ttatcatttg
    52981 caagccagag aaatcccttg ctatcgagta aacctatcgc taggattagc aatcattatc
    53041 atttagatta ctatcacgat tatggcctat cgttccgcac cgctctatga agatgtcatc
    53101 tggcgaacgc atctccagcc gcaggatgct gggcttgcac aagccgtacg ggcgatgatc
    53161 gccaaacatc gtgaacattt gctggagttt atccgcctgg atgaacccgc tccgcttaac
    53221 gccatgacgc tggcgcaatg gtcatcaccg aatgcgctaa gttctctact ggcggtctat
    53281 tccgatcata tctatcgcaa ccaaccaacg atgatccgcg agaacaaacc gctgatctca
    53341 ctatgggcac aatggtatat cggcctgatg gtgccaccat taatgctggc gctactgacg
    53401 caggaaaaag cattagatgt gtcgccggaa catttccatg ctgagtttca cgaaaccgga
    53461 cgcgtcgcct gtttctgggt cgatgtgtgt gaagataaaa acgcaacacc acattcgccg
    53521 cagcagcgaa tggaaacgtt aatcagccag gcgctggttc cggttgtgca agcactagaa
    53581 gcgaccggag agatcaacgg caaacttatc tggagtaata ccggttattt gatcaactgg
    53641 tatctcactg agatgaagca actgctcggc gaggctactg ttgaatcgct gcgccatgcc
    53701 ctcttttttg agaaaacgct cacgaacggt gaagataatc cactctggcg taccgtggta
    53761 ctgcgcgacg gcctgctggt gcgccgcact tgctgccagc gttatcgctt accggatgtg
    53821 cagcaatgtg gctattgtac gctgaaatga aagcactgcc ggggaagtaa acccggcatc
    53881 atgcggatta atagggatgc aacacattac ttgcttcgca atgccgcata cataacagaa
    53941 tatcttcggc ctccaacaac cctgcatcag aactgacgcc cagtttcgac atcacattaa
    54001 atttatgcgc tcggatcgtt ttgatattac gtttaagctg ctcggcaatt tgtgtcattg
    54061 agtagccacg cgacataaag cgcaatattt ctctctccgt cgggcttaac gtccggcttt
    54121 ggttaatgta ccactgattg ttcagtcggt cggtcgcctg acgtacacca tttaatgaca
    54181 aaaagagttc ctgatgaaaa atctccagcg ttgacgcttt acttaataca ccgtccagcg
    54241 gtgatggcga caatgaacca atcagccgag cttcaatatc atcatccaca ataactaaac
    54301 gccgggtacg cggaaactta atcgccagct cagtcaggca agataatccc tctctgcgct
    54361 cacttctcat ggcagaaaaa gaaaaaatga ccgccgcaaa cgagatccgc gacatggcat
    54421 cctgaaatga ctcctgactg gtataggtat ggagcttata agggttgccc gcaaactttc
    54481 tgaataaact ctcaataccg atactactca tgacgcattt ttctatgagt gcgacatttc
    54541 tcttcttaat tcggctgtgt tccattctgc ggcatctcca taaacagaag gcaagttaag
    54601 ttctcttgca ccctgcgcgc tatttatcca tgcatacatt tcagcgttgc tgtgaattgc
    54661 cagtcgacgc atcgcactgt ttttttgggc gctgatagtt ttattacttt tcttaagcaa
    54721 tgaagctatc tggttgatgc cccatccttt acctaatagc cgcagtacct tgcgctctga
    54781 aagagtgaga atgacggagc gatagctaaa atcatgagtt tcagtcattg ccggagaggt
    54841 cagcatggcg ctgatacgct ctgcgtgcgt attgccggaa cgtacggtct tgaccagatt
    54901 ctcaatgggt tcaacatcag acaataacgt cgccgtaggg cacatgagca gttctacagc
    54961 ctgggaatac caggagcgtg acaccatgaa aacccagtga atttcccgat attgtgagat
    55021 taaagaataa taatgttcac aaacagaacg tggatcttca ctttcaccgg ctaaatcag
//
LOCUS       DAJTQQ010000018        53039 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000018 DAJTQQ010000000
VERSION     DAJTQQ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53039
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.018"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..150)
                     /gene="atl"
                     /locus_tag="OO309_001179"
     CDS             complement(<1..150)
                     /gene="atl"
                     /locus_tag="OO309_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein"
                     /protein_id="HCR5846388.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLP"
     gene            313..409
                     /gene="ffs"
                     /locus_tag="OO309_001180"
     ncRNA           313..409
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO309_001180"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            529..882
                     /locus_tag="OO309_001181"
     CDS             529..882
                     /locus_tag="OO309_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR5846389.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(924..2474)
                     /gene="pdeB"
                     /locus_tag="OO309_001182"
     CDS             complement(924..2474)
                     /gene="pdeB"
                     /locus_tag="OO309_001182"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR5846390.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2638..3108)
                     /locus_tag="OO309_001183"
     CDS             complement(2638..3108)
                     /locus_tag="OO309_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR5846391.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3224..3775)
                     /gene="maa"
                     /locus_tag="OO309_001184"
                     /pseudo
     CDS             complement(3224..3775)
                     /gene="maa"
                     /locus_tag="OO309_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(3947..4165)
                     /gene="hha"
                     /locus_tag="OO309_001185"
     CDS             complement(3947..4165)
                     /gene="hha"
                     /locus_tag="OO309_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5846392.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4191..4565)
                     /gene="tomB"
                     /locus_tag="OO309_001186"
     CDS             complement(4191..4565)
                     /gene="tomB"
                     /locus_tag="OO309_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR5846393.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5110..8259)
                     /locus_tag="OO309_001187"
     CDS             complement(5110..8259)
                     /locus_tag="OO309_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR5846394.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8282..9475)
                     /gene="acrA"
                     /locus_tag="OO309_001188"
     CDS             complement(8282..9475)
                     /gene="acrA"
                     /locus_tag="OO309_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR5846395.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9617..10264
                     /gene="acrR"
                     /locus_tag="OO309_001189"
     CDS             9617..10264
                     /gene="acrR"
                     /locus_tag="OO309_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR5846396.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10392..13754
                     /gene="mscK"
                     /locus_tag="OO309_001190"
     CDS             10392..13754
                     /gene="mscK"
                     /locus_tag="OO309_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR5846397.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(13966..14127)
                     /gene="rsmS"
                     /locus_tag="OO309_001191"
     CDS             complement(13966..14127)
                     /gene="rsmS"
                     /locus_tag="OO309_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR5846398.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14141..14668)
                     /gene="priC"
                     /locus_tag="OO309_001192"
     CDS             complement(14141..14668)
                     /gene="priC"
                     /locus_tag="OO309_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR5846399.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14738..15115
                     /locus_tag="OO309_001193"
     CDS             14738..15115
                     /locus_tag="OO309_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR5846400.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15268..15819
                     /gene="apt"
                     /locus_tag="OO309_001194"
     CDS             15268..15819
                     /gene="apt"
                     /locus_tag="OO309_001194"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR5846401.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            15948..17873
                     /gene="dnaX"
                     /locus_tag="OO309_001195"
     CDS             15948..17873
                     /gene="dnaX"
                     /locus_tag="OO309_001195"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR5846402.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17206..17270
                     /gene="dnaX"
                     /locus_tag="OO309_001195"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            17926..18255
                     /locus_tag="OO309_001196"
     CDS             17926..18255
                     /locus_tag="OO309_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR5846403.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18255..18860
                     /gene="recR"
                     /locus_tag="OO309_001197"
     CDS             18255..18860
                     /gene="recR"
                     /locus_tag="OO309_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR5846404.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            18970..20844
                     /gene="htpG"
                     /locus_tag="OO309_001198"
     CDS             18970..20844
                     /gene="htpG"
                     /locus_tag="OO309_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR5846405.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21025..21669
                     /gene="adk"
                     /locus_tag="OO309_001199"
     CDS             21025..21669
                     /gene="adk"
                     /locus_tag="OO309_001199"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR5846406.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21801..22763
                     /gene="hemH"
                     /locus_tag="OO309_001200"
     CDS             21801..22763
                     /gene="hemH"
                     /locus_tag="OO309_001200"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR5846407.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22760..23719)
                     /gene="aes"
                     /locus_tag="OO309_001201"
                     /pseudo
     CDS             complement(22760..23719)
                     /gene="aes"
                     /locus_tag="OO309_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            23871..25175
                     /gene="gsk"
                     /locus_tag="OO309_001202"
     CDS             23871..25175
                     /gene="gsk"
                     /locus_tag="OO309_001202"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR5846408.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25308..26984)
                     /locus_tag="OO309_001203"
     CDS             complement(25308..26984)
                     /locus_tag="OO309_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR5846409.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27222..28442)
                     /gene="fsr"
                     /locus_tag="OO309_001204"
     CDS             complement(27222..28442)
                     /gene="fsr"
                     /locus_tag="OO309_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR5846410.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIEPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28660..30312
                     /gene="ushA"
                     /locus_tag="OO309_001205"
     CDS             28660..30312
                     /gene="ushA"
                     /locus_tag="OO309_001205"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5846411.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30349..30828)
                     /gene="ybaK"
                     /locus_tag="OO309_001206"
     CDS             complement(30349..30828)
                     /gene="ybaK"
                     /locus_tag="OO309_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR5846412.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31032..31826)
                     /locus_tag="OO309_001207"
     CDS             complement(31032..31826)
                     /locus_tag="OO309_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR5846413.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            31964..32305
                     /locus_tag="OO309_001208"
     CDS             31964..32305
                     /locus_tag="OO309_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5846414.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32520..35024)
                     /gene="copA"
                     /locus_tag="OO309_001209"
     CDS             complement(32520..35024)
                     /gene="copA"
                     /locus_tag="OO309_001209"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR5846415.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35286..36218
                     /gene="glsA"
                     /locus_tag="OO309_001210"
     CDS             35286..36218
                     /gene="glsA"
                     /locus_tag="OO309_001210"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR5846416.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36221..37513
                     /locus_tag="OO309_001211"
     CDS             36221..37513
                     /locus_tag="OO309_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5846417.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37638..38045
                     /gene="cueR"
                     /locus_tag="OO309_001212"
     CDS             37638..38045
                     /gene="cueR"
                     /locus_tag="OO309_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR5846418.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38046..38504)
                     /locus_tag="OO309_001213"
     CDS             complement(38046..38504)
                     /locus_tag="OO309_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR5846419.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38501..39418)
                     /locus_tag="OO309_001214"
     CDS             complement(38501..39418)
                     /locus_tag="OO309_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5846420.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39564..40241
                     /gene="fetA"
                     /locus_tag="OO309_001215"
     CDS             39564..40241
                     /gene="fetA"
                     /locus_tag="OO309_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR5846421.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40228..41007
                     /gene="fetB"
                     /locus_tag="OO309_001216"
     CDS             40228..41007
                     /gene="fetB"
                     /locus_tag="OO309_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR5846422.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41070..41924)
                     /gene="cnoX"
                     /locus_tag="OO309_001217"
     CDS             complement(41070..41924)
                     /gene="cnoX"
                     /locus_tag="OO309_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR5846423.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(41985..42794)
                     /gene="ybbO"
                     /locus_tag="OO309_001218"
     CDS             complement(41985..42794)
                     /gene="ybbO"
                     /locus_tag="OO309_001218"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5846424.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42784..43406)
                     /gene="tesA"
                     /locus_tag="OO309_001219"
                     /pseudo
     CDS             complement(42784..43406)
                     /gene="tesA"
                     /locus_tag="OO309_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43377..44063
                     /locus_tag="OO309_001220"
     CDS             43377..44063
                     /locus_tag="OO309_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5846425.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQLFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44060..46475
                     /locus_tag="OO309_001221"
                     /pseudo
     CDS             44060..46475
                     /locus_tag="OO309_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47188..48282)
                     /gene="mnmH"
                     /locus_tag="OO309_001222"
     CDS             complement(47188..48282)
                     /gene="mnmH"
                     /locus_tag="OO309_001222"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR5846426.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48351..49277)
                     /gene="allS"
                     /locus_tag="OO309_001223"
     CDS             complement(48351..49277)
                     /gene="allS"
                     /locus_tag="OO309_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR5846427.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49507..49989
                     /gene="allA"
                     /locus_tag="OO309_001224"
     CDS             49507..49989
                     /gene="allA"
                     /locus_tag="OO309_001224"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR5846428.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGIQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50067..50882
                     /gene="allR"
                     /locus_tag="OO309_001225"
     CDS             50067..50882
                     /gene="allR"
                     /locus_tag="OO309_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR5846429.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            50972..52753
                     /gene="gcl"
                     /locus_tag="OO309_001226"
     CDS             50972..52753
                     /gene="gcl"
                     /locus_tag="OO309_001226"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR5846430.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52766..>53039
                     /locus_tag="OO309_001227"
     CDS             52766..>53039
                     /locus_tag="OO309_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR5846431.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12794 a        13638 c        13970 g        12637 t
ORIGIN      
        1 agggagacgc tttaacacac cgcccacctg gcgcgcggcg cggggcgatc ccgccagttt
       61 cgccacatca ccgtaagtgg tgacatagcc ttcgggaata gcggcgacga tttgccagac
      121 gcgttgggga aatgaatctt ctttttccat cttttcttcc tgaggtaatt tttcagcata
      181 atctggaaaa acgcccgagt gaagtcgcat tgcgcaagaa accagcatct ggcacgcaat
      241 gggttgcaat tagccggggc agcagtgata atgcgcctgc gcgttggttt tcaacgctct
      301 caatgggggc tctgttggtt ctcccgcaat gctactctgt ttaccaggtc aggtccggaa
      361 ggaagcagcc aaggcagatg acgcgtgtgc cgggatgtag ctggcagggc ccccacccat
      421 ttctgcctcc caccgtttcg tcaaaaaatc ccagcatggc taaactttaa ccacaactga
      481 cgtcgcaaga attgtctggc tgcgcagtac gcttcggagg tatgtctgat gaagtatgtt
      541 gatggttttg tggttgccgt tcctgccgat aaaaaggatg cctatcggga aatggccgct
      601 aaggccgcgc cattgtttaa agagtttggc gcgcttcgta ttgtcgaatg ctgggccagc
      661 gatgtgccgg atggcaaagt gaccgatttt cgtatggcgg tgaaagcgga agagaatgaa
      721 gaggttgtct ttagctggat tgaataccct tcaaaagagg tccgcgacgc agctaatcaa
      781 aagatgatgt cggacccacg gatgaaagag ttcggcgagt ccatgccgtt tgacggcaag
      841 cgaatgatct atggcggatt cgagtcaatc atcgacgaat aggtagcgtg acgggctgtg
      901 cggcgtgccg tcagcccaaa aattcacaaa tgttgctcgg cccagcgtaa gaaatcttct
      961 ttcggtaatg ccttgctgta gagccagccc tgaccgtagt gcacgccatg ctggcgtagc
     1021 cactcttctt gtttactggt ttcgattccc tccgctacca ttttcagttt cagtgttttt
     1081 gccatttcga tgatatgcgg cgtgacattt ttatattcca gcgcatcaac gaaagattta
     1141 tcgatcttca gaatgtcgac atccagatcc tgtaaataac ttaaacttga ataccccgta
     1201 ccaaaatcat caagataaat ttcatggccc gcctcccggt agcgagaaat tatcggggcg
     1261 ctggttttcg gatcggcaaa ctcgcgttca gtgagttcaa gcgcgatctg tctgggatta
     1321 acctgatagt gattgatcat ttcacgcagc aattgcggga ttttttccga ggtgagcacg
     1381 gtggattcaa gattgatcga aatatgctgc tgtggatgct gacgcaagca gtcgcccata
     1441 tcttcaaaga cgcttcttat aatcagtagc gtcaatggct cagaaaggcc cgtttgctgt
     1501 gccagcggaa taaaactatc tggtgacaac caactaccgt ctgtctgcgg ccagcgcgcc
     1561 agtgcctcag caccgacaat tttgccattg gctaaggaga caatcggctg atagtgcacg
     1621 caaatatcac gattttcgat agcatcctgc agccgatgat gcggtgattg gatacggcgc
     1681 aaaatacgca gcacaaacat cgctgccagc aggccaatca ccaacccgag cggtaaccaa
     1741 ataaagactt gccgatgcca gcctttctgc aacattttcg ttgaagccca ggtgatgatc
     1801 gaaatattca tctccaggaa gggcagtata tcgtaaatga tcccattatt ttcgatatgc
     1861 tcaccgggtg ttttttgtag cctggtaata attccctgag caatttcatc gctgctggtt
     1921 atgacaacgt tatgggcatt gccaataatg gcggcatcaa tttgccatga actataggga
     1981 atgacatcaa taaaggaagc ggggtcgatc atgacgacat aatgtgccgt tcccatggcg
     2041 accatgtaac ggataatgcc taaatcgtta tgcgatgtta accagacacg ataaccatct
     2101 ttcgaaattt tacctagctc gggaaaggta tcgggcggac tttcatgctc cagagacgaa
     2161 cactggggaa cgttgttatc gatataaacc acttcctgaa tatagcgata actgtaagat
     2221 acccgacgca tttccatgag atgggcttcg ctacaggcag cgccttgcca tctttccagc
     2281 tcctgcagcg catctttccc ttgtgtcgcc accttattgg ctcgaatagc gacgcgggag
     2341 gaataggtat ccagctcttc aataaacgat gtttctacct gctgatgggc cagccagatg
     2401 cttaagccga caggcagcaa tactgaaaga atcagtactc ccgaaataag gccgaccaga
     2461 tgtcgtgttc tcaccagcat atccttatta acacagtgta gggaaagtat atctgattgt
     2521 ggaaaaaggc tggcaggaga taaggaggta gagctggtat ttcatgaagt gatgggtgga
     2581 aacgatcatt gtcatttgac caggcgcaga gctttacgcc tggtatgtaa ccctaactta
     2641 agatgttgat tcggcgcgag cgagggtaaa aatatcgtaa aaagacagtt cacctttacg
     2701 gacgatcatt ttttgcagtt gttccttatg aacatccgcg acacgcggag agttcaagat
     2761 ggcttcaacc agcgcggcag gtacaaagcg cgtgttgtac cattcgccgt tatagcaaac
     2821 gcggaaatcc agcacgtcga tatcttcata gcggtagcgt gggtagacat cgaaaaagaa
     2881 gaaaccatta agcaggataa ataatacaac cagtagccag acagagcctg acagcgtttc
     2941 ggactgcaac atcaccgcca gggtggcaaa aaaagcaacg tacataccga taaacagccc
     3001 cggatgttta cggataaaac tgatactaaa gcggggtttg ttgtcgcgtt tttcgcgggt
     3061 attcagagac tcaatcgttt cggtcagcag gcgttgtatt tcggtcatcg tttgccttcg
     3121 tggtatctgc aactttccag ggaacaccct attttaacgt gggaatagta gaggggaagt
     3181 aacagatcta ccacaatttt gcataacagt tgcgaaaaac cgattacaat tttttaatta
     3241 ttctggctgg attaccgccc acgacaacgt tgtccgggac accttttgtg acaactgcac
     3301 ctgaagctac cacgacgtta tcaccaatgg tcacaccagg gttaatgacc gcgcgtccgc
     3361 caatccagac gttattaccg atggtgacgg gtttccccag ttcagcaccg ctattacgtg
     3421 ctacagggtc gatgggatgt gttgccgtgt agatatgaac gcctggtgcc aacatacagt
     3481 tatcaccgat gcgaataggg cagacatcaa gcatcacgca atcgaagttg gcgaaaaaat
     3541 tattgccgag aaaaatgtta tagccataat cacagcgaaa cgttggctca atataagcct
     3601 ctgtcacctg accgaataaa ttagcgagaa tttactggcg taatgtgtgc tcttccgcca
     3661 gggaatgatt gtatcggtga ataagctgac gagcgcgcag gcgatcgcga gataacgtct
     3721 catctgccga gcgatacaac tcaccagcaa tcatcttttc tttttctgtg ctcattactg
     3781 acctccggtt gctgaaaatg cggcaacaca gtaagacgaa aagtcagtga agagaacgtt
     3841 ctcattacat ctgtgtgata aagatcacat agggtatgtc ttcatggcga aaaagtataa
     3901 aattcttaat aaacagccgg ttatagcacc gaaagcgaat tatttattag cgaataaatt
     3961 tccatactga ggaagggatc ttgtcgtaca gtttattcat ggtcaattcg gcgaggcggt
     4021 gatctgcggc tgagtaaaat accgccagtt cattatctga taattcgtat ttatttttct
     4081 cgataacacg ctccagcgtg tcaattgtct ggcaacgacg taaacgcatt aaataatcgg
     4141 tttttgttaa aggtttttcg gacatacttc tacccatggt tgtaataatt ctaacaagat
     4201 aaactcgcag gattctcttt cgtcgcgttg acaaaacaac ggaatagtcg attacctgac
     4261 ttccgccatt tctgaagagc ctgcatatta ataccataac tgctgaacaa cataaaggtg
     4321 tcatccagat attcgtcgat ctgctcaatg agcttattgt cttcattata cttaattttg
     4381 taattaagtg cgaaggtcgc aatatgctca atcagttcat tcaactggag gttgatcgcc
     4441 gaggttgggt cgttaaccca accatgattg ctttcttcaa ggtttgcaag acagtcatga
     4501 tacagggttt cacagagaaa cttaagctgt gcgatatcat gtcttttggg tgagtattca
     4561 tccataacgc gtccccttct tagtggttga actaacggac acctttcggg atggaaaaaa
     4621 cttactgacc tagacttgcc ctctcgtttg ttagcttaac tataagccac tctttgcagg
     4681 ttttcatcgc attattacga aacattacaa ttaggaagga atcgggacag gactcacttt
     4741 ttcatactag cactaacccg cagcaggtaa aagcagtttg ctcgaactgt tcataacatt
     4801 tcaggattat cgccagatga aattggtgac actaatacca ggattgctct gaatatgacg
     4861 taataaccga ggaatgaata aagaattacc gcaaataatt aagaatagcc tctaaatgat
     4921 tatggattat taaatctatt agcgctactt aatataaatt aactaacaat cagtattttt
     4981 tatgaatttt atacgtggtt aatactggtt ttcgtatgag atcctgagtt ggtggttcaa
     5041 ttactcctta atgttcgtag gttatgcata aaaaggccgc ttacgcggcc ttagtgatta
     5101 catgttgtat caatgatgat cgacagtatg gctgtgctcg atatcttcat tcttgcggct
     5161 aaagcggcgg cgaaccacca caaagaatac cggaacgaag aagattgcca gtaccgttgc
     5221 ggtcaccatc ccgcccatta caccggtacc tactgcgttc tgcgcgccgg aaccagcacc
     5281 agtactgata accagcggca taacgccgag gataaacgcc agcgaggtca tcaggatcgg
     5341 acgtaaacgc atccgcaccg catcaagcgt cgcttcaatc agacctttac cttctttatc
     5401 catcaagtct ttggcgaatt cgacgataag tatcgcgttc ttcgccgaca acccaatggt
     5461 tgtgagcagt cctacttgga agtaaacgtc attggtcagg ccacggaagg tggcagccag
     5521 caacgcaccg ataaccccca gcggaacgac cagcataacg gagaacggag tcgaccagct
     5581 ctcgtacagc gccgccagac acaggaatac gacaatcaac gaaatcgcgt acagtgaagg
     5641 tgcctggttg ccggagagac gttcctgata ggacatcccc gtccagtcat agccaacacc
     5701 ggtaggcagt ttgctcgcca gttgttccat cagctccatt gcttcaccgg tacttttacc
     5761 cggtgccgcc tggcctaaga tttccatgga tggcaggccg ttgtaacgtt ccagacgcgg
     5821 cgaaccgtac tcccaacgag aagaggagaa cgccgagaat ggcaccatct gaccatcagc
     5881 agcacgaaca taccagtcgc cgatatcatc cggcagcata cggtatttcg cttctgacat
     5941 gacgtaaact ttcttcacac gaccgcggtc gataaagtcg ttcacatagc taccgcccca
     6001 tgcagcgccc agagtggtgt taatgtcgtt gatagaaaca cccagcgcct gcgctttttc
     6061 ctggtcgata tcaatcttaa actgcggggt atcttccaga ccgtttggac gtacgctggt
     6121 cagcatatca gggtgcttcg ctgcttctgc aagcagctgg ttacgcgcct gagtcagttt
     6181 ttcgtgacca aggccagcct ggtcaatcag ctcaaagtca aagccggttg cagtacccag
     6241 ttccacgatt gcgggcaggt taaaggcgaa aaccatcgca tctttgattt gcgagaaagc
     6301 gcgtgttgca cgcatggtaa tcgcttcaac tttgttttct tcgcccggac gatcggccca
     6361 gtccttcaag gaaacgaacg caataccggt attctgacca cgtcccgcaa agccgaagcc
     6421 gttaacggcg aacaccgact caacgttgtt cttttctttg gtcagatagt aatgcgttac
     6481 ctcattgagc actttctgcg tacgttcctg cgttgcacct gctggcagct gaaccatggt
     6541 cataaatacg ccctggtcct catctggcaa gaaggagctt ggcagacgca cgaacagata
     6601 ggccatgccg accacgatga tcagatacag cactaggtaa cgccccgtac tgcgcagaat
     6661 accgcctacg ctgtcggtgt agtggtgcgt gctcttctcg aacatgcggt tcaaccagcc
     6721 gaagaagcct tttttacctt ccccgtgatc gcctttggca atcggtttca gcatggtggc
     6781 acaaagagcc ggagtcagga tcaacgctac cagtaccgac agcgccattg ctgaaacaat
     6841 ggtaatagag aactgacgat agatagcacc agtagaaccg ccaaagaagg ccatcggtac
     6901 gaataccgcc gacagtacca tcgcgatacc gaccagagcg ccctgaatct gccccatcga
     6961 cttacgggta gcttcttttg gcggcaaacc ttcttccgcc ataacacgct ccacgttttc
     7021 taccacaacg atagcgtcat ccaccaacaa gccgatggcg agcaccatcc cgaacattgt
     7081 tagcgtgttt atcgagaagc caaaggcggc aaggacggca aaggtcccga gcaataccac
     7141 cggtacggca atggtcggaa tcaacgtcgc gcggaagttc tgcaggaaca gatacataac
     7201 caggaacacg aggatgatcg cttcgaccag cgttttaacc acttcgtgaa tagagatttt
     7261 cacgaacggc gtggtgtcgt acgggtaaac aattttcaga cccgacggga agaacggttc
     7321 catcttcgcc agttcagcac ggattgccgc agcggtatcc agcgcgtttg caccggtcgc
     7381 cagcttgatc cccagaccgg aagcaggttg gccgttaaac tctgcgatga tgtcgtagtt
     7441 ctcaccaccc agctcaatct tcgccacatc acgcagcagt acgcgggaac catcctgatt
     7501 cactttcagc aggattttgc cgaactcttc agtagaggtc agacgcgtct gagcaataat
     7561 agaggcgtta agctgttggc ctttcaccgg cggcgtacca ccgagctgac ccgccgcaac
     7621 ctgggcgttc tgcgctttga tggcggtaat gacatcaacc ggcgttagct ggaatttgtt
     7681 cagctcattc gggttcatcc agatacgcat cgcgtactgt gaaccgaaca actgaacatc
     7741 acccacgccc gacgtacggc tgatggcatc tttcatattc gccgctacgt agtcagagat
     7801 atcctcctgc gtcatggtgc catcggtgtt gataacgccg acaaccatca ggaagctgct
     7861 ggatgatttc tcaacgctca ccccttgctg ctgaacttct tgcggcagca acggcatcgc
     7921 cagctgcagt ttgttctgca cctgaacctg cgcgatatcc gcatcagtac cagactcaaa
     7981 ggtcagggtg atctgcacgg tacccgtgga gtcactgtta gaggacatgt acatcaggtt
     8041 atcgataccg ttcatattct gttcgataac ctgtgtcacc gtgtcctgca ctgttttcgc
     8101 atcagcgccg gggtaggagg cggagatcgt tactgccggc ggtgcaatcg taggatattg
     8161 cgccaccggc agtttgagga tcgccagccc ccctgccaac atgatgataa tggcgatcac
     8221 ccacgcaaaa atcgggcgat cgataaagaa attaggcatg tcttaacggc tcctgtttaa
     8281 gttaagactt ggactgttca ggctgagcac cgcttgcggc ttgctggtta ttatcagcgg
     8341 taacttcttg tgcttttacc tggacaccag gacgcacttt ctgcagccca cttattacta
     8401 cgcgatcgcc tgctttcaga ccttctgtca ccagccactt atcgccaata gcctggcttg
     8461 caacgatcgg acgggtttcc actttgtcat ccgcgccaac taccagtacg gtggcatcac
     8521 cacgcggcgt acgggttacg ccctgttgcg ggactaaaat agcgtttgga ttaagccctt
     8581 cttccagacg tgcgcgcacg aacatacccg gcagcagagt gtgatccggg ttcgggaaga
     8641 tagcgcgtag ggtgatagac ccagtggtct gatcaacggt aacgtcagag aattccagcg
     8701 taccgtcctg cgggaactta atgccgtcac tggtgatcag cgacactttg gctttgccgt
     8761 tctcttgttt cagcgtgcca ttcgccagtt cctgtttcag gcgaaggaag tcgttgctgg
     8821 actgggtcac atcaacgtag atcggatcaa gttgctgcac ggttgccagc acagtcgcct
     8881 gaccgttctg taccaatgcg ccttccgtca cgttcgactt accaatgcga ccgctaatcg
     8941 gagaggtgac tttggtgtaa gccagattga tccgcgcagt ttcaacggca gctttcgccg
     9001 cagttaccgc agcattcgcc tgttgcgcat cagccagagc ctgatcgtac ccttgcttac
     9061 tgatgtactg agtaccgagc agtttctgat aacgattcac cgtcaattgc gcgatattgg
     9121 ctgcagcctg ggctttcgcc agatcacctt tcgcactgtc gtatgtcgcc tgataggtcg
     9181 caggatcaat ctgatagaga gagacaccag cttcgatgtc gctaccttct ttgaaattac
     9241 gcttcaggat aatcccgcta acttgaggac gaacttctgc gatccggtag gcactggtgc
     9301 gacctggaag ctcggttgtg atctgcagag gttcagtttt gactgttact acgccaacgg
     9361 cgggcatctg ctggccacct tgttgggcct gtttgtcgtc acatcctgtt agggctaagc
     9421 tgcctgagag catcagaacg accgccagag gcgtaaaccc tctgtttttg ttcatatgta
     9481 aacctcgagt gtccgatttc aaattggtca atggtcaaaa gttaataaac ccattgctgc
     9541 gtttatatta tcgtcgtgct atggtacata cattcacaaa tgtatgtaaa tctaacgcct
     9601 gtaaattcac gaacatatgg cacgaaaaac caaacaagaa gcgcaagaaa cgcgccaaca
     9661 catcctcgat gtggctctac gtcttttctc acagcagggg gtatcatcca cctcgctggg
     9721 cgagattgca aaagcagctg gcgttacgcg cggtgcaatc tactggcatt ttaaagacaa
     9781 gtcggatttg ttcagtgaga tctgggaact gtcagaatcc aatattggtg aactagagct
     9841 tgagtatcag gcaaaattcc ctggcgatcc actctcagta ttaagagaga tattaattca
     9901 tgttcttgaa tccacggtga cagaagaacg acgtcgatta ttgatggaga ttatattcca
     9961 caaatgcgaa tttgtcggag aaatggctgt tgtgcaacag gcacaacgta atctctgtct
    10021 ggaaagttat gaccgtatag aacaaacgtt aaaacattgt attgaagcga aaatgttgcc
    10081 tgcggattta atgacgcgtc gggcagcaat tattatgcgc ggctatattt ccggcctgat
    10141 ggaaaactgg ctctttgccc cgcaatcttt tgatcttaaa aaagaagccc gcgattacgt
    10201 cgccatctta ctggagatgt atctcctgtg ccccacgctt cgtaatcctg ccactaacga
    10261 ataaccctga atctgactcc aggatttttc ctggacattt tcgtcgttgc tattctggtt
    10321 cactgcgtcg tgatattctt gcggtttgac tttttcaggt cgttcttcag gttcagaaac
    10381 cttcattcat catgactatg ttccagtatt acaaacgatc acggcatttt gttttttcag
    10441 catttattgc ttttgttttt gtcttgttat gccagaacac ggcgtttgcg cgggcgtcat
    10501 cgaatggtga tctgccgaca aaagcggacc tgcaggcgca acttgactca ctaaataaac
    10561 aaaaagatct ttctgctcag gacaaactgg tgcagcagga tctgacagat acattagcca
    10621 ccctcgataa aatcgatcgc ataaaagaag agacagttca gctacggcaa aaagtcgctg
    10681 aagcgccgga aaaaatgcgc caggcgaccg cggcgttaac agcacttagc gatgtcgata
    10741 acgacgaaga aacgcgcaaa attctgagta cgctttcgtt gcgccagctg gaaactcgcg
    10801 ttgcccaggc gctggacgat ttgcaaaacg cacaaaacga tctggcgtct tataacagcc
    10861 agctggtttc gttacagacg cagcccgaac gcgtgcaaaa tgcgatgtat aacgcttcgc
    10921 agcagctgca acaaattcgc agtcgtctgg atgggactga tgtcggcgag actgccttac
    10981 gtcccagcca gaaagtgtta atgcaggctc agcaggcgtt gctgaatgcg gagattgacc
    11041 agcagcgtaa aagcctggaa gggaacaccg tcttgcagga taccttgcaa aagcaacgtg
    11101 attacgtgac ggcgaacagc gcccgtctgg agcaccagtt gcagctgttg caagaagcgg
    11161 taaacagcaa gcgcctgact ttaactgaaa aaacggcgca ggaagccgtc tccccggatg
    11221 aagccgcgcg tattcaggct aatccgctgg tgaaacagga actggaaatt aaccagcagt
    11281 taagtcagcg tctgattacc gcgactgaaa acggtaatca gttgatgcag caaaacatta
    11341 aagtcaaaaa ctggctggag cgggcgctgc aatcggaacg caatattaaa gagcagattg
    11401 ccgtcctgaa gggcagcctg ctgttgtctc gtatccttta ccagcaacaa caaacgctgc
    11461 cctcggcgga tgaactggaa aacatgacca accgcatcgc ggatttgcgt ctcgaacagt
    11521 ttgaagttaa ccagcagcgt gatgcactct tccagagcga tgcgttcgtc aacaaactgg
    11581 aagaaggtca caccaacgaa gtcaacagcg aagttcacga tgcgttattg caagtggttg
    11641 atatgcgtcg cgaattgctg gatcaactca acaaacagtt gggtaaccag ctgatgatgg
    11701 ccattaacct gcaaatcaac cagcagcagt taatgagcgt atcgaaaaac ctgaaatcca
    11761 tcctgactca gcaaatcttt tgggtgaaca gtaaccgtcc aatggactgg gactggatca
    11821 aagcgttccc gcaaagcctg aaagatgaat ttaagtcgat gaaaatcacg gtgaactggg
    11881 aaaaagcctg gcctgccgtt tttatcgctt tcctcgccgg tttgccgctg ctgttgattg
    11941 ccgggctgat ccactggcgt ctgggctggc tgaaagcgta tcaacagaaa ctggcttccg
    12001 ctgtgggttc cctgcgtaac gatagccagc tcaacacacc aaaagcgatc cttatcgacc
    12061 tgatccgcgc gctgccggtg tgcctgatta ttctcgcggt tggcctgatt ctgttgacca
    12121 tgcagctcaa catcagcgaa ctgctatggt cgttcagtaa aaaactggcg attttctggc
    12181 tggtgtttgg cctgtgctgg aaggtactgg agaaaaatgg cgttgccgtg cgtcacttcg
    12241 gcatgccgga gcagcagacc agccactggc gtcggcaaat tgtccgcatc agtctcgcgt
    12301 tgctgcctat ccatttctgg tctgtggtgg cagaactttc cccgctgcat ctgatggatg
    12361 atgtgctggg gcaggcgatg attttcttca acctgctgct gattgccttc ctggtatggc
    12421 caatgtgccg cgaaagctgg cgtgataaag agtcgcacac catgcgacta gtcaccatta
    12481 ccgtgctgtc gattatcccg attgcattga tggtgctgac tgccacgggt tatttctaca
    12541 ccacgctgcg cctctccggg cgttggattg aaaccgttta tctggtgatc atctggaact
    12601 tgctctacca gacggtattg cgcggcttaa gcgtagcggc gcggcgtatc gcctggcgtc
    12661 gtgcgctggc gcgtcggcag aatctggtga aagagggcgc agaaggtgcc gaaccgccgg
    12721 aagaacccac cattgcactg gagcaagtta accagcagac gctgcgtatt accatgttgc
    12781 tgatgtttgc gctgttcggt gtcatgttct gggcaatttg gtccgatttg atcaccgtgt
    12841 tcagctatct cgacagcatc acgctctggc attacaacgg cactgaagct ggcgctgcgg
    12901 tggtgaaaaa cgtcaccatg ggcagtctgt tgtttgcgat tatcgcctca atggtggcct
    12961 gggcgttgat tcgcaacctg cctggtttac tggaagtgct ggtgctctcg cgactgaata
    13021 tgcgccaggg cgcgtcgtat gccattacta ccatccttaa ctacatcatt attgccgttg
    13081 gtgcgatgac ggtgttcgga tcgctgggcg tctcttggga taaactccag tggctggccg
    13141 cagcattatc cgtaggtctt ggttttggtt tacaagaaat tttcggtaac ttcgtctccg
    13201 gtttgatcat tctgttcgaa cgtccggtgc gtattggcga tacggtaacc attggtagct
    13261 tctcggggac ggtaagtaag atccgtattc gtgcgacaac gattaccgat ttcgatcgca
    13321 aagaagtgat catcccgaac aaagcgtttg ttaccgagcg tctgatcaac tggtcgttga
    13381 ctgacactac tacgcgtctg gtgatccgtc tcggcgtggc ctatggctcc gatctggaaa
    13441 aagtgcgtaa agtgttactg aaggcggcga ctgagcaccc aagggtgatg cacgaaccaa
    13501 tgccggaagt cttctttacg gcatttggtg ccagcacgtt ggatcatgag ctgcgtctgt
    13561 atgtgcgtga actgcgtgac cgtagccgta ctgtcgatga gctgaaccgt actatcgatc
    13621 agctgtgccg tgaaaacgac atcaacattg cctttaacca gcttgaagtg catctgcaca
    13681 acgagaaggg cgatgaggtg acggaagtaa aacgcgacta caaaggcgac gatccgacac
    13741 cagcggttgg gtaataacca aagggggcga catttagttg ccccgagatt actgacaaag
    13801 tgcgcgttgt tcatgccgga tgcggcgtga ccgccttatc cggcctacga aaccgcaaga
    13861 attcaatata ttgcaggagc ggtgtaggcc tgataagcgt agcgcatcag gcagttttgc
    13921 gtttgcccgc aatcttaggg gacatttagc ggccccattt atttctcact tttccgcctc
    13981 atcatcgtgc gttaatttct tttcataatc acgctttaca atatccagcg cgcgcagcac
    14041 ggtactggca gggatctgat tttcctccag cagcacaatc aaatcgacag ccagtttgac
    14101 atcgtcaggg gcattttcaa gtgacatact ctttccattg ctagcgggtt aagcgcgcta
    14161 gcctgttttc gattttttcc agcgcatggc ggcagcgtgc caggcgcgct tcataggctt
    14221 ccacttcacg atgcagcgtt tgctgttcca cgagatcggt cacccgcgcc aggcgggctc
    14281 tgcgttcggc aaccatctca cgtagccgcc gctcaaaatc ctgatgctga atacgtttac
    14341 gctgccagcg ggaaattttc ggtggcgcac tgtcccactc gcgcaatgac caggcggagg
    14401 cttcacgcgc gatggcttcc agttgtgccg ccagatgttc cgccagccag gccacttgcg
    14461 gcagctgttg ctgctcaact gcatgacgaa gcgcagccag attatcgccc gcctcgtcga
    14521 gacaagactg tagtgttgtc gcacgagtct gaaaaagatg cctgtcgaaa cgagcactta
    14581 gcgtggcgaa ctgtgacacc ggggcacaac gctgacgcag cgtagcgagc tgtccttcca
    14641 gtttttccag cagcagggcg gttttcacga taagacctca atgaaaatga taattgttat
    14701 gctaaagtag ccactatgta agctgacaac actcaatatg caacgaatca ttttaatcat
    14761 cattggctgg ctggcggtag tgctgggtac gctgggcgtg gtattaccgg tattaccgac
    14821 gacgccgttt atcctgctgg cggcctggtg ctttgcccgt tcttccccgc gctttcacgc
    14881 ctggttgctg taccgctcat ggtttggcag ctatctacgt ttctggcaga aacatcatgc
    14941 gatgccgcgc ggcgtcaaac cgcgggcgat tttgcttatt ttgctcacgt ttgccatttc
    15001 tctgtggttc gtccagatgc catgggtgcg catcatgttg ctggtaattc tcgcctgttt
    15061 gcttttctat atgtggcgaa ttccggtgat tgatgaaaag caagaaaagc actgaagcac
    15121 aacaatcgca gttgcaatta ttgcgtacag ccagtacatt ctggcgtttt cgagcacagg
    15181 cgcaggcggt caaaggttaa acaacagtta cttttgatac gtttaaaacg cgccgtgagt
    15241 accaccgtaa caagcaggca tacacttatg accgcgactg cacagcagct tgagtatctc
    15301 aaaaatagca tcaaaagcat tcaggactac ccaaaacccg gcattctttt ccgcgatgtc
    15361 accagcttac tggaagaccc gaaagcttac gctctcagca tcgacttgct ggttgagcgt
    15421 tacaaaaatg cgggcattac caaagttgtc ggcaccgaag cgcgtggctt cttgtttggc
    15481 gctccggtag ctctgggtct gggcgttggc tttgtaccgg tccgtaaacc gggcaaactg
    15541 ccgcgtgaaa ccatcagtga aaattacgac ctggaatacg gcaccgatca gctggaaatc
    15601 cacgttgatg ccatcaaacc gggcgacaaa gttctggtgg tggacgatct gctggcaacc
    15661 ggcggcacta tcgaagcgac cgttaaactg atccgtcgtc tgggtggtga agtggctgac
    15721 gctgcgttca ttatcaacct gtttgatctc ggcggcgaac agcgtctcga aaaacagggc
    15781 attaccagct acagccttgt cccgttcccg ggccattaat tatcgccagt cttgtgctgc
    15841 ccacgctacg gacagcacaa gatgtgcatt cagcctcgcc gttctgacgg ggctgtgtta
    15901 gcattacccc ttcgtgaatc caccttccag cgtttcagag cctgccaatg agttatcagg
    15961 ttttagcccg aaaatggcgc ccacaaacct ttgctgacgt cgtcggccag gaacatgtgc
    16021 tgaccgcact ggcgaacggc ttgtcgttag ggcgtattca tcatgcttat cttttttccg
    16081 gcacccgtgg cgtcggaaaa acctctatcg cccgactgct ggcgaagggg ctaaactgcg
    16141 aaaccggcat taccgcgacg ccgtgcggcg tgtgcgataa ctgtcgtgaa atcgagcagg
    16201 ggcgctttgt cgatctgatt gaaatcgacg ccgcctcgcg caccaaagtt gaagataccc
    16261 gcgacctgct ggataacgtc cagtacgctc cggcgcgtgg tcgtttcaaa gtttatctga
    16321 tcgacgaagt gcatatgctg tcgcgccaca gctttaacgc actgttaaaa acccttgaag
    16381 agccgccgga gcacgttaag tttctgctgg cgacgaccga tccacagaaa ttgccggtga
    16441 cgattttgtc acgctgtctg caatttcatc tcaaggcgct ggatgtcgag caaattcgcc
    16501 atcagcttga gcacatcctc aatgaagaac atatcgctca cgagccgcgg gcgctgcaat
    16561 tgttggcgcg cgccgctgaa ggcagcctgc gagatgcctt aagtctgacc gaccaggcga
    16621 ttgccagcgg tgacggccag gtttcaaccc aggcggtcag tgcgatgctg ggtacgcttg
    16681 acgacgatca ggcgctgtcg ctggttgaag cgatggtcga gggcaacggc gagcgcgtaa
    16741 tggcactgat taatgaagcc gctgcccgtg gtatcgagtg ggaagcgttg ctggtggaaa
    16801 tgctcggctt gttgcatcgt attgcgatgg tacaactttc gcctgctgca cttggcaacg
    16861 acatggccgc catcgagctg cggatgcgtg aactggcgcg caccataccg ccgacggata
    16921 ttcagcttta ctatcagacg ctgttgattg gtcgcaaaga attaccgtat gcgccggacc
    16981 gccgcatggg cgttgagatg acgctgctgc gcgcgctggc gttccatccg cgtatgccgc
    17041 tgcctgagcc agaagtgcca cgccagtcct ttgcacccgt cgcgccaacg gcagtaatga
    17101 cgccaaccct ggtgccgccg caatcagcgc cgcagcaggc accgactgta ccgctcccgg
    17161 aaaccaccag ccaggtgctg gcggcgcgcc agcagttgca gcgcgtgcag ggagcaacca
    17221 aagcaaaaaa gagtgaaccg gcagccgcta cccgcgcgcg gccggtgaat aacgctgcgc
    17281 tggaaagact ggcttcggtc accgatcgcg ttcaggcgcg tccggtgcca tcggcgctgg
    17341 aaaaagcgcc agccaaaaaa gaagcgtatc gctggaaggc gaccactccg gtgatgcagc
    17401 aaaaagaagt ggtcgccacg ccgaaggcgc tgaaaaaagc gctggaacat gaaaaaacgc
    17461 cggaactggc ggtgaagcta gcggcagaag ccattgagcg cgacccgtgg gcggcacagg
    17521 tgagccaact ttcgctacca aaactggtcg aacaggtggc gttaaatgcc tggaaagagg
    17581 agagcgacaa cgcagtatgt ctgcatttgc gctcctctca gcggcatttg aacaaccgcg
    17641 gtgcacagca aaaactggct gaagcgttga gcatgttaaa aggttcaacg gttgaactga
    17701 ctatcgttga agatgataat cccgcggtgc gtacgccgct ggagtggcgt caggcgatat
    17761 acgaagaaaa acttgcgcag gcgcgcgagt ccattattgc ggataataat attcagaccc
    17821 tgcgtcggtt tttcgatgcg gagctggatg aagaaagtat ccgccccatt tgatcgtaag
    17881 cacagcttac gttcgtcatc cttaacgtga ttgagagaga aacctatgtt tggtaaaggc
    17941 ggtctgggta acctgatgaa gcaagcccag cagatgcaag aaaaaatgca gaaaatgcag
    18001 gaagagatcg cgcagctgga agttaccggc gaatctggcg caggtctggt aaaagtgacc
    18061 atcaacggtg cacacaactg ccgtcgcgta gagatcgacc cgagcctgct ggaagacgac
    18121 aaagagatgc tggaagacct ggtggctgca gcattcaacg acgcagcacg tcgtattgaa
    18181 gaaacgcaga aagaaaaaat ggcctctgta tcctccggaa tgcagctgcc gcctggcttt
    18241 aagatgccgt tctgatgcaa accagcccgc tgttaacaca gcttatggaa gcactgcgct
    18301 gtctgccggg cgttggcccg aagtcggcgc agcgtatggc gttcacgctg cttcagcgcg
    18361 atcgtagcgg cgggatgcgt ctggcgcagg cgctcacccg ggcgatgtcg gaaatcggcc
    18421 actgcgccga ttgccgcact ttcaccgaac aggaagtctg taacatctgt tcgaatccgc
    18481 gtcgtcagga aaacggtcaa atctgtgtgg tggagagtcc ggcggacatc tacgccattg
    18541 agcagacggg gcagttttct ggtcgttatt ttgtgttgat ggggcatctg tcaccgctgg
    18601 acggcatcgg tccggatgat atcgggcttg atcgtctgga acagcgtctg gcagaggaaa
    18661 aaatcactga agtgatcctc gccaccaatc ccacggttga aggtgaagct accgctaact
    18721 acattgccga gctttgcgcg caatatgacg tggaagccag ccgaatcgct catggcgtac
    18781 cggttggcgg cgaactggaa atggtcgatg gcaccacgct gtcacactcc ctcgccgggc
    18841 gtcataagat tcgtttttaa gcaaacgaga gcaggagcac ctgctctcgc ttgaaattat
    18901 tctcccttgt ccccatctct ccctcatcct gtttttaacc ttaaaatggc attattgagg
    18961 tagacctaca tgaaaggaca agaaactcgt ggttttcagt cagaagtgaa acagcttctg
    19021 cacctgatga tccattctct ctattccaat aaagaaatct tcctgcgtga gcttatctct
    19081 aacgcctccg atgcggcgga caagctgcgt ttccgtgcgc tatctaaccc ggacctgtac
    19141 gaaggtgatg gcgaactgcg cgttcgtgtc tctttcgata aagacaagcg cacgctgacc
    19201 atctccgata acggcgtggg gatgacccgc gacgaagtga ttgaccatct ggggactatc
    19261 gctaaatccg gtaccaaatc attcctcgaa tccctgggtt ctgaccaagc gaaagacagc
    19321 cagctgatcg gtcagtttgg tgttggtttc tactctgcgt ttatcgtggc cgacaaagtg
    19381 accgtgcgta ctcgcgcggc aggcgaaaaa ccagaaaacg gcgtcttctg ggaatcggct
    19441 ggcgaaggtg aatataccgt tgccgatatc accaaagaag atcgtggtac tgaaatcacc
    19501 ctgcatctgc gtgaaggcga agacgagttc ctcgatgact ggcgcgtgcg ttccatcatc
    19561 agcaaatact ccgaccatat cgcgctgccg gtagagatcg aaaaacgcga agagaaagat
    19621 ggcgaaaccg ttatctcctg ggcaaaaatc aacaaagcgc aggcgctgtg gactcgtaac
    19681 aagtcggaaa tcaccgatga agagtataaa gagttctaca aacacatcgc ccacgacttt
    19741 aacgatccgc tgacctggag ccacaaccgc gttgaaggta agcaggagta caccagcctg
    19801 ctgtacatcc cgtcccaggc tccgtgggat atgtggaacc gtgatcataa acacggcctg
    19861 aaactgtacg ttcaacgcgt gtttatcatg gacgacgctg aacagttcat gccgaactat
    19921 ctgcgcttcg tgcgtggtct gattgactcc agcgatctgc cgctgaacgt ttcccgtgaa
    19981 atcctccagg acagcacggt aacgcgtaac ctgcgcaatg cgctgaccaa gcgtgtgctg
    20041 caaatgctgg aaaaactggc gaaagacgac gcggaaaaat accagacctt ctggcaacag
    20101 tttggcctgg tactgaaaga aggtccggcg gaagatttcg ctaaccagga agcgatcgcc
    20161 aaactgctgc gttttgcttc tacttatacc gattcttctg cgcagaccgt gtctctggaa
    20221 gactacgttt cccgcatgaa agaagggcag gagaaaatct actacatcac tgcagacagc
    20281 tatgcggcag cgaagagcag cccgcacctg gaactgctgc gtaagaaagg catcgaagtt
    20341 ctgctgcttt ccgaccgcat cgatgagtgg atgatgaact atctgactga gttcgacggt
    20401 aaaccgttcc agtcggtgtc taaagttgac gagtcgctgg aaaaactggc tgacgaagtt
    20461 gatgagagcg cgaaagaagc ggagaaagca ctgactccgt tcatcgaccg cgtgaaagct
    20521 ctgctcggcg agcgcgtgaa agatgtccgt ctgactcacc gtctgaccga tacgccagcg
    20581 atcgtttcga ccgacgcgga cgaaatgagc actcagatgg cgaaactgtt cgctgcggcg
    20641 ggccagaaag tgccagaagt gaaatacatc ttcgaactga acccggatca cgtactggtg
    20701 aaacgtgcgg cagatactga agatgaagcc aagttcagcg agtgggtaga actgctgctg
    20761 gatcaggcgc tgctggcaga acgcggcacg ctggaagatc cgaacctgtt tattcgtcgt
    20821 atgaatcagc tgctggtttc ctgatgtcat gccggatgac ctttgtgtca tccggcattt
    20881 ttcttttcat catctgtact ttccgcaaat tatctcgcca ttaaccgttt cagcctcagg
    20941 tgcctttctt gaggcaatcg cctgttggtg gtatcgttta tcgctttttc aaaaaaatcg
    21001 acacatttta aggggatttt cgcaatgcgt atcattctgc ttggcgctcc gggcgcgggg
    21061 aaagggactc aggctcagtt catcatggag aaatatggta ttccgcaaat ctccactggc
    21121 gatatgctgc gtgctgcggt caaatctggc tccgagctgg gtaaacaagc aaaagacatt
    21181 atggatgctg gcaaactggt caccgacgaa ctggtgatcg cgctggttaa agagcgcatt
    21241 gctcaggaag actgccgtaa tggtttcctg ttggacggct tcccgcgtac cattccgcag
    21301 gcagacgcga tgaaagaagc gggcatcaat gttgattacg ttctggaatt cgacgtaccg
    21361 gacgaactga ttgttgatcg tatcgtaggc cgccgcgttc atgcgccgtc tggtcgtgtt
    21421 tatcacgtta aattcaatcc gccgaaagta gaaggcaaag acgacgttac cggtgaagaa
    21481 ctgactaccc gtaaagacga tcaggaagaa accgtgcgta aacgtctggt tgaataccat
    21541 cagatgactg caccgctgat cggctactac tccaaagaag cggaagcggg taacaccaaa
    21601 tacgcgaaag ttgacggtac caagcaggtt gctgaagttc gcgctgatct ggaaaaaatc
    21661 ctcggctaat caagccgcag cagatctgct ttcgggcagg tctgcttatc cacctcaggc
    21721 aatacctctc acagcaatta gttcttcttc ctcacttttc cgctacaatt atcaacaagt
    21781 tgaatcgata agaggcggta atgcgtcaga ctaaaaccgg tatcctgctg gcaaacctgg
    21841 gtacgcccga tgcccccaca cctgaagcgg taaaacgcta tctgaaacaa tttttaagcg
    21901 acagacgcgt ggttgatacc tcacggttgt tatggtggcc gttgctgcgc ggcgtgattt
    21961 tgccgctgcg ctcgccgcgt gtggcgaagc tgtacgcctc tgtctggatg gaagacggct
    22021 cgccgctgat ggtttacagc cgccagcaac agcaggcgtt ggcacaacgt ttaccggata
    22081 cgccagtggc gctgggcatg agctacggct cgccatcact ggaaagctcc gtcgatgaac
    22141 tcctggcaga gcatgtagat catattgtgg tgctgccgct ttatccgcaa ttctcctgtt
    22201 ctacggtcgg tgcggtatgg gatgaactgg cacgcattct ggcgcgcaaa cgtagcattc
    22261 cggggatatc gtttattcgt gattacgccg ataaccacga ttacattaat gcactggcga
    22321 acagcgtacg cgcttctttt gccaaacatg gcgaaccgga tctgctgctg ctctcttatc
    22381 atggcattcc ccagcgttat gcagatgaag gcgatgatta cccgcaacgt tgccgcacaa
    22441 cgactcgcga actggcttcc gcactgggga tggtaccgga aaaagtgatg atgacctttc
    22501 agtcgcgctt tggccgggaa ccctggctga tgccttatac cgacgaaacg ctgaaaatgc
    22561 tcggagaaaa aggcgtaggt catatacagg tgatgtgccc gggctttgct gcggattgtc
    22621 tggagacgct ggaagagatt gccgagcaaa accgtgaggt cttcctcggt gcgggcggga
    22681 aaaaatatga atatattcca gcgcttaatg ccacgccgga acatatcgaa atgatggcta
    22741 atcttgttgc cgcgtatcgc taaagctgag cggtaaagaa ctgagcgccg tcgcgaagag
    22801 cctcgtcggc ggttttcatc atccgtgaat aatgcaaaaa ggcatgcagc gtacctgggt
    22861 agagtttgaa ctcacagggc tgctgatgcg ccgctaacgt ctagtaaagc agacggctgt
    22921 catccagcag cggatcgaac tccgccccgg caataaaaca gggcggaact tcgcgagtga
    22981 gatcattatt aaacagacag taatacggtg actcgcggtc cgcgtcgttg cttaaatacg
    23041 cctcttcata catctgcaaa tcctgttgcg ttaagccatc ccagacaccg cccaacagac
    23101 gacgagtcac ggaatcccgt aatccgtaaa gcccatacca cagcaaaaca cccgcaattt
    23161 taccgcaatc gatctgttta tcacgcaacc acaacgcact ggcgagcgcc agcatggcac
    23221 ctgcggaatc accggcaaag ccaatgcggg acatattgat ttgataatcc tccgcctgct
    23281 ggtggaaata acaacaagca gccacaattt cctctatcgc ttgtggaaaa cgcgcttcag
    23341 gtgaaagggt gtaattaata ccaatcaccg tacattggct gtagcttgcc agcaggcgca
    23401 tgatgcgatc gtgggtatcg agattgccga gaataaaacc gcctccatgc aaataaaata
    23461 gcgtcgccgg gctatctggc tgcggacaaa agagacgtgt ttccacctgc ccatattttg
    23521 ttggaaccat gtaagctctg gttgccattt ctggagcgcc cgcattccag aatcggcgct
    23581 caagcgtgta atactgtcgt tgctcagcaa tcgttcccgt tgcgggccag gacggtaaat
    23641 ccggctgaag agtattcaca acggtcttca tttcagcaga aataaggtcc agaacaggta
    23701 gtttgttttc cggcttcata caaaaactcc tttcaaatta cgtcattgta aggaaccact
    23761 gccatgaaaa tgcgatcccg cctgctgata ttgaaactgg ctgcgtctcg cgcgctcccg
    23821 tcagattgtg ttaacattcg ccgctcagtt aaccacccgt aaaaacaacc atgaaatttc
    23881 ccggtaaacg taaatccaaa cattacttcc ctgtaaatgc acgcgatccg ctgcttcagc
    23941 aattccagcc agaaaacgaa accagcgccg cctgggtcgt aggtattgac cagacgctgg
    24001 tcgatattga agcgaaagtg gatgatgaat tcattgagcg ttatggatta agcgccgggc
    24061 attcactggt gattgaggat gacgtagccg aagtgcttta tcaggagcta aaacagaaaa
    24121 acctgattac ccatcagttt gcgggtggca ccattggtaa caccatgcac aactactcgg
    24181 tgctcgcgga cgaccgttcg gtgctgctgg gcgtcatgtg cagcaatatt gaaattggca
    24241 gttatgccta tcgttacctg tgtaacactt ccagccgtac cgatcttaac tatctacaag
    24301 gcgtggatgg tccgattggt cgttgcttta cgctgattgg cgagtccggg gaacgtacct
    24361 ttgctatcag tccaggccac atgaaccagc tgcgggctga aagcattccg gaagatgtga
    24421 ttgccggagc ctcggcactg gttctcacct catatctggt gcgttgcaag ccgggtgaac
    24481 ccatgccgga agcaaccatg aaagccattg agtacgcgaa gaaatataac gtaccggtgg
    24541 tgctgacgct gggcaccaag tttgtcattg ccgagaatcc gcagtggtgg cagcaattcc
    24601 tcaaagacca cgtttctatc cttgcgatga acgaagatga agccgaagcg ttgaccggag
    24661 aaagcgatcc gttgttggca tctgacaagg cgctggactg ggtagagctg gtgctgtgca
    24721 ccgccgggcc aatcggcttg tatatggcgg gctttaccga agacgaagcg aaacgtaaaa
    24781 cccagcatcc gctgctgccg ggcgctatag cggaattcaa ccagtatgag tttagccgcg
    24841 ccatgcgcca caaggattgc cagaatccgc tgcgtgtcta ttcgcacatt gcgccgtaca
    24901 tgggcgggcc ggaaaaaatc atgaacacta atggagcggg ggatggcgca ttggcagcgt
    24961 tgctgcatga cattaccgcc aacagctacc atcgtagcaa cgtaccaaac tccagcaaac
    25021 ataaattcac ctggttaact tattcatcgt tagcgcaggt gtgtaaatat gctaaccgtg
    25081 tgagctatca ggtactgaac cagcattcac ctcgtttaac gcgcggcttg ccggagcgtg
    25141 aagacagcca ggaagagtct tactgggatc gttaagttat cgtcggttcg taggccagat
    25201 aaggcgttca cgccgcatct ggcatttggc tctcgatgcc tgatgcgacg ctggcgcgtc
    25261 ttatcatgcc tacatatttt tcatatttta catccggcaa ccaccgttta ccccgtcacc
    25321 acttcacccg ccggtggcgt ttccagcagt tccagcatag tacgggcaat ttcacgttca
    25381 cccatcacta cctgattcgc gccacgttcg gtgatatacg tcacttcatc gtcataatgg
    25441 gcgcgggcga taatctcaat atccgggttt ttcgcgcggg cagacgccac aatctcaccc
    25501 gcttcataac cgttggggat cgtcaggatc agccattttg cacattccag atgtgccagt
    25561 tgcataattt cttcgttcgc tgcattgccc aatacagcgc ggaccccacg ctctcgcagc
    25621 tcatcaacac gggttcgtga cgtctcaatc accaccagcg gaatatcaga ggcgagcaat
    25681 ttctccccca gcaggctgcc tacacgaccg taacccacca atagcgcatg gttgcaaata
    25741 tccactggga tctgtttctc ttcttcgatt gcctcttcca gcgtctgctc ttccagcgtt
    25801 tcggtcttcg ccagatattt ctccagtagt gcgaacagta ccgggttgag cataatcgac
    25861 aggatcgccc ctgccagtac caggttttgt ccggcctgcg gcagtaaatt caatgccatt
    25921 cccagtcccg ccaggataaa cgcgaactca ccaatctgcg ccaggctggc ggcgatggtt
    25981 aatgccgtac gttgggagtg accaaacaga cggacaagga acagggccgc gaccgattta
    26041 ccaaacagaa taattgccag cgtcgccagc actgccagcg gttgctgaat cagaattaac
    26101 ggatcaaaca acatcccgac tgagacaaaa aacagcaccg caaacgcgtc gcgcaatggc
    26161 aacgtatcgt gggcggcacg gtgactcaat tcagactcgt tcagtaccat cccggcaaag
    26221 aacgcaccga gtgcaaagga gacatcaaac agctctaccg caccaaaggc aatccctaac
    26281 gccagcgcca gcactgacag ggtaaacagc tcgcgagaac cggttgccgc gctgcgtgcc
    26341 ataatccacg gcaccagacg gcgacctacc agcatcataa tggcgataaa tgcgatcacc
    26401 ttgccgatgg tgatccccat atcgactgca agagtggcaa agcccacatc gccctgttcc
    26461 atcattcctg ccactgcggg cagcaacacc agcgtcagaa ccatcaccag gtcttccaca
    26521 atcaaccaac cgatggcgat ttgcccacgc tgactgtcta ttaattgccg ttcttcaagt
    26581 gcgcgcagta acaccacggt actggcggtg gaaagacata agccgaacac gataccggtc
    26641 attaacgacc agcccagcac ggcggagagc gccataccca gcagcgtcgc cacggctatc
    26701 tgggcgatcg cgccgggaat ggcgatggac tttaccgcca tcaaatcctt gagcgaaaaa
    26761 tgcaaaccga cgccgaacat cagcaaaata acgcccagtt cagccagttc cggcgcaagc
    26821 ttggtatcgg caacaaagcc cggagtgaat ggtcctgcca gcacacccgc taacagatat
    26881 cccaccagag gagaaatacg tagtttattg gccagcatgc cgaggataaa ggcgagcaca
    26941 aggccgccaa caatggtggt gataagcggg gtggcgtgat gcattccgtc tccttttcct
    27001 ggtggttatt gtccattttt ggctgggaaa accaaaatta caggtaatag tttatgacaa
    27061 tttcattgat gatgtttatg aataattgtt gaattttgca gaaaaatgga attagctgca
    27121 aaaaaagcac ggttcggaaa acagaaggcg gtttaacaga ggaaaggctt atggcgactg
    27181 taaaacgatg cagccaaagt ttgcctttgg ctgcaatgaa atcagtcttt atgccggtta
    27241 tcaggcagga atatggtcaa catccccaat agtggcagga aagcacagat tttatagact
    27301 aactcgatgc tggtgtgatc ggcgataagc cccagaactg ccgctcccag acctcccatg
    27361 ccgaaagcaa aaccgaaaaa gagtccagaa accataccga tacgtcccgg aagtagctcc
    27421 tgagcgtaga ccagaatggc agagaatgcc gaagcgagga taaatccaat aatcaccgtt
    27481 aaaacccccg tccagtgcag gcgggcgtag ggtaaaatca gcgtaaacgg cgcaacgccg
    27541 aggatagagc cccaaatcac atatttccgt ccaattttat cccctacagg cccgccgatc
    27601 accgtacctg ccgcaacggc aaacaggaag gcaaacagat gaagctgggc attctggata
    27661 gataatccga atttttgcat cagataaaag gtgtaatagc tgctgatgct cgccatatag
    27721 aaatatttcg agaaaatgag gattaacaga atgctgactg ccagtaccac tttgttgcgc
    27781 ggcagtggat tgattatcgt cgctttgggt tttcctttat tcattcggtg ctgtgccgag
    27841 taccaacggc taatttgcgc caacaccacg atcgccagca gtgccgcaag cacaaaccag
    27901 gcaacgttgc ctttgccata aggctcgata atcaccgccg ccagcaaggg tcccagggaa
    27961 ctgccaaagt tgccgccgac ctgaaagata gattgcgcca ggccatgccg cccgccggaa
    28021 gccatacggg ctacgcgaga agattccgga tgaaagaccg atgaaccggt accgaccagc
    28081 gccgctgcca gcagaactgc gccaaaactg cccgccagcg caagcagcac cagaccactt
    28141 aaggtaaagc acatgccaat tggcaacgac cacggcatcg gatatttatc ggtccagtag
    28201 ccgaccactg gttgcagtag cgaagaggcg agctggaagg tgagggttat catgccaatc
    28261 tgcataaatg tcagagaaaa ttctgactga agcagcggat aaatcgccag aatcagcgat
    28321 tggatcatgt cgttcagcag atgtgagagg ctgatagcac ctaaaatacc aaacgatgtt
    28381 cgggccttgg tcgttgacgc agccgcgccc gccacaggct gggtttgttc actcattgcc
    28441 ataggaaagt cactttttca gggttgcgat gtaaagaatg atcttatttg tgattattac
    28501 ccgactaaca tacctgtatg cgtcgtctga aggaagtctc aacgccgaat acagaatttc
    28561 taatctggat gcagatttat cttcaccgga cgcagacttg tctatgatgt tgcgtcatac
    28621 tatttttcaa cacgttgaaa tcaggtcagg gagagaagta tgaaattatt gcagcggggc
    28681 gtggcgttag cgctgttaac cacatttaca ctggcgagtg aaactgctct ggcgtatgag
    28741 caggataaaa cctacaaaat tacagttctg cataccaatg atcatcatgg gcatttttgg
    28801 cgcaatgaat atggcgaata tggtctggcg gcgcaaaaaa cgctggtgga tggtatccgc
    28861 aaagaggttg cggctgaagg cggtagcgtg ctgctacttt ccggtggcga cattaacact
    28921 ggcgtgcccg agtctgactt acaggatgcc gaacctgatt ttcgcggtat gaatctggtg
    28981 ggctatgacg cgatggcgat cggtaatcat gaatttgata atccgctcac cgtattacgc
    29041 cagcaggaaa agtgggccaa gttcccgttg ctttccgcga atatctacca gaaaagtact
    29101 ggcgagcgcc tgtttaaacc gtgggcgctg tttaagcgtc aggatctgaa aattgccgtt
    29161 attgggctga caaccgatga cacagcaaaa attggtaacc tggaatactt cactgatatc
    29221 gaatttcgta agcccgccga tgaagcgaag ctggtgattc aggagctgca acagacagaa
    29281 aagccagaca ttattatcgc ggcgacccat atggggcatt acgataatgg tgagcacggc
    29341 tctaacgcac cgggcgatgt ggagatggca cgcgcgctgc ctgccggatc gctggcgatg
    29401 atcgtcggtg gtcactcgca agatccggtc tgcatggcgg cagaaaacaa aaaacaggtc
    29461 gattacgtgc cgggtacgcc atgcaaacca gatcaacaaa acggcatctg gattgtgcag
    29521 gcgcatgagt ggggcaaata cgtgggacgg gctgattttg agtttcgtaa tggcgaaatg
    29581 aaaatggtta actaccagct gattccggtg aacctgaaga agaaagtgac ctgggaagac
    29641 gggaaaagcg agcgcgtgct ttacactcct gaaatcgctg aaaaccagca aatgatctcg
    29701 ctgttatcac cgttccagaa caaaggcaaa gcgcagctgg aagtgaaaat aggcaaaacc
    29761 aatggtcgtc tggaaggcga tcgtgacaaa gtgcgttttg tacagaccaa tatggggcgg
    29821 ttgattctgg cagcccaaat ggatcgcact ggtgccgact ttgcggtgat gagcggaggc
    29881 ggaattcgtg attctatcga agcaggcgat atcagctata aaaacgtgct gaaagtgcag
    29941 ccattcggca atgtggtggt gtatgccgac atgaccggta aagaggtgat tgattacctg
    30001 accgccgtcg cgcagatgaa gccagattca ggtgcctacc cgcaatttgc caacgttagc
    30061 tttgtggcga aagacggcaa actgaacgac cttaaaatca aaggcgaacc ggtcgatccg
    30121 gcgaaaactt accgtatggc gacattaaac ttcaatgcca ccggcggtga tggatatccg
    30181 cgccttgata acaaaccggg ctatgtgaat accggcttta ttgatgccga agtgctgaaa
    30241 gcgtatatcc agaaaagctc gccgctggat gtgagtgttt atgaaccgaa aggtgaggtg
    30301 agctggcagt aatccgaaag tgccggatgt ttgcatccgg cacaatgctt aatcgcggcg
    30361 ggcgatatca gcaaatttgg catcgaggat ctttgccaga tcgcctgccg ccagttcgat
    30421 atccagtccg cgcttgccgc cggaaacata aatagtggca aattcttgtg cgggggcgtc
    30481 gataatcgtt ggcagacgtt ttttctgccc cagtgggcta atccccccaa ccaggtatcc
    30541 cgtcgaacgc tgcgcgacca tcggatcggc catctcaact ttcttggcac ccagcgcttt
    30601 tgctactttt ttaagatcca gttgacctgc gaccggcgta acggccacgg caaggtgttt
    30661 catatcaccg ttcactgcca ccagcagcgt tttgtagacc tgatccggat tcaaacctaa
    30721 ttttttgacg acttcatcgc caaaattggt ttcagccgga tcgtgctcgt aggtatggat
    30781 ttgaaacgaa atcttgtttt tttcgagtaa tttaactgcg ggtgtcatgg taattttcct
    30841 gccttaaaca aaaaaacgaa gcaagcatac gcctgatggc ggtccaaaaa aaagagtcat
    30901 cttgcctaag agtattggca ggatggtgag attgagcgac aatcgagtta caccgtcgct
    30961 taaagtgacg gcataataat aaaaaaatga aattcctctt tgacgggcca atagcgatat
    31021 tggccatttt tttagcgcaa catttgcggc aaattccctt ctccatacag gtgtagtgca
    31081 ccgaccgcga ccacatatcg ccccggcggc atggcgcgta atttatcccg ccaggcgaga
    31141 tttcgctgat gcatcagcac atcgtacagc gactgactga acgtattggg cagcgttata
    31201 tcattatttt gcggcggtgc attcagccac cagctcatca tttgttgcag caaccgtgcg
    31261 ttggtatgcc agtgggtcag cgtatcgtcc agcagcgcca gtcctttgtc agggagctgg
    31321 agcaacatgg caatctggtt ttcagcccct tccagttcaa tcacgggttt atgttgttgc
    31381 ttcgccgcct gcaatagctg gtaatcgata ccgtattctg cccgcagccc cagtttttgt
    31441 gcctgcgtcg cctgaagaac catcgcgatt tgccacagcg gttgggtaga aaagagtgag
    31501 ggagaaatgc ccatctcctg gctaatgtgc tgcaggtttt ggagttgctc ctcgctaatg
    31561 cgctcttcca gcgcctcgca ggcaggcaaa ttagcaaaag gcgtatcgct ggtggaaaca
    31621 tccgcctcga cgatcagcgc atcggcgttt ttgagctttt tgagcaaacg ggtgggcagg
    31681 ggagccatat cgtggctacc catatgaata ctgccaatca gatgaaaatg gcgattgccg
    31741 gggagggtga tatcgatggc aggccaggtg taatgattac cgcgcagcgc ggcccaaagc
    31801 gtttttaccc ggtacaacag atccatacga cctccctttg tgaaatatca tgctagcgcg
    31861 cggtgaggga tggcgcaaat ggagttgggg acttgtttaa tgtgtttgta tgattcagta
    31921 tgttcttgca tcgctattca cagggaagca acagttaaaa accatgaaac aggcaacaag
    31981 aaaaccgacg acacccggag atattcttct ctatgaatat ctggaaccgc tcgatttgaa
    32041 aatcaatgag ttagcagagt tgctgcatgt tcatcgtaat cgcgtcagtg cactgatcaa
    32101 taacaatcgt aaactcacca ctgagatggc attccgtctg gcgaaagttt ttgataccac
    32161 agtcgatttt aggctaaacc tccaggcggc agttgatctt tgggaagttg aaaacaacat
    32221 gcgcacccag gaagaattgg gacggattga aacagtggct gaatatttgg cacgccgtga
    32281 agagcgtgca aaaaaggtcg cgtaaggcca aaacatcgct tcatctcgta ggccggataa
    32341 ggcgttcacg ccgcatccgg catccgagca ccattgcctg atgcgacgct tacgcgtctt
    32401 atcaggccta caaacctgtg cctgaaccgt aggccggata aggcgtttac gccgcatccg
    32461 gcagtcatgc gtcgatgcca aatgcgccgc cctaaagcag cgcatccgca atgacgtact
    32521 tattccttcg gtttaaaccg cagcaaccgg ttggcgttac ttactacggt aattgacgag
    32581 agcgccattg ccgctccggc aactaccggg ttaagcagtg ttccagtaaa cggccacaaa
    32641 ataccggcgg cgaccggaat accgatgctg ttgtagataa aggcaccgag caggttctgc
    32701 ttcatgttgc gcagcgttgc gcgggaaatc gcgagcgcat ccgcaacgcc catcaggcta
    32761 tggcgcatca gggtaatcgc cgcggtttca atggcaacat cactgccgcc acccatcgca
    32821 atgccgacat ccgcctgggc cagcgctggc gcgtcgttaa tgccgtcgcc caccattgcc
    32881 acctgacgtc cttcactttg cagacgtttg atcgcttcgg ctttaccgtc cggcagcact
    32941 ccggcaatca cttcatcaat tccagcttct ttggcgatcg cattggcggt ggttgggtta
    33001 tccccggtca acatcaccag acgatatccc gctttatgca ggcgttgcag cgccgccacg
    33061 ctatcactac gcaacggatc gcgtacagcc agcagggcta ctgctttccc gtcaatcgcc
    33121 agcagcacag gcgttgctcc ttgcgatgcc tgagctgaaa tatcggcttc aatcgcttta
    33181 gtatcgactt gctgatcatt taacaacgcc tgattaccca gcaataacgc atgaccttct
    33241 gcttcaccgc tcacgcccag cccgcgcaat gtgcggaaac cgttgacctg cggtagctgc
    33301 atatcgcctg ctttatcaag gatcgctcgt gccaatggat ggctggaacc ttgctccagc
    33361 gccgccgcca gacgcagcgc ctgcgcttcg tcaacatcag caaatgtttt cactgcgaca
    33421 acctgcggct tcccttcggt cagcgtcccg gttttatcga acacgacggt atcgagtgta
    33481 ctggcgcgtt gcagcgcgtc ggcgtcccgc accagcacgc caaactcagc cgcccgcccg
    33541 acgccggaaa taatcgacat cggcgtcgcc agtcccagcg cacacgggca ggcgataatc
    33601 agcaccgtag tggcaatcac cagggtatag acaatctgcg gtgccggacc aaagaaatac
    33661 cagattgccg cactgacaag cgcaataacc actactaccg gcacaaatat ggctgagatt
    33721 ttatccgcca gctgaccgat ttctggcttg ctgctctggg cctggcgcac catgcgaatg
    33781 atccttgata gcgtggtatg gctgccaacc gcactggcac gaaacagcac actgccgtcc
    33841 tgtaccactg tcccggcatg gacgctatcg ccttcgcctt tttgctgcgg gattggttcg
    33901 cccgtcagca tcgcttcatc cagccatgct tcgccctggg taatctcgcc atctaccggc
    33961 acgcgatcgc cggtcgtcag gcgcagcaac atacctggct gcacttctgc cagaggcacg
    34021 cttttttcac cttcgtcagt aaccaggcgc gccgtcggtg gggttaaatc gagtaatttc
    34081 tccagtgcct tagaagaacg ctgacgtgcg cgtgcttcca gcatatggcc gagattgatc
    34141 agaccgataa tcatcgcact ggcttcatag taaagatgtc gcgcttccat cgggaaccac
    34201 tgcggccaca ggttgacgct catcgaatag agccacgcca cgcccgttcc cagcgccacc
    34261 agcgtatcca tcgtcgctgc accgttcagc aggcttttcc atgcactgcg gtaaaaatgg
    34321 ccgctggcga aaaccatcac cgccagggtt atcaggccga taaccaacca caggctgcgg
    34381 ttgtcggcgg tgactatcat gttatcgccg atcatccccc agaccatcac cgggataccc
    34441 accgccagtg cgacaattgc ctgccagcgg aagcgcttca tcgtagcgac ggcggtttct
    34501 tgctggcgtt cgcggcgttt cgcgtcatct tcaatcgctt ccgcgccgta gcccgctttt
    34561 tccaccgcct gcactaaatc ttgtggggag gcactgccca tcaccagcgc agtacgctcc
    34621 gccaggttta cccgtgcctg agtgacgccc ggtacgcttt gcagcgcatt ttgtacgcgg
    34681 gtgacacagc tggcgcagct catgccgctc agcagcaact gctggctgtc atcgtcatcg
    34741 gcggtcgctg ccggaagcgc ctcagaaacc gctgtcagtg cttccgacgg gattgatgac
    34801 tccgccagcg gtttagcctt tgggtggctt acagatgcgt cataacccgc ttgtttgatg
    34861 gtttcgatca gctgttctgc actggcagtc ccggtaacgt gcgcttcagt gatagacaca
    34921 tccgcttgtt ccacatccgg gcgctgttca agactttctt tcacgcgttt aacgcagtga
    34981 ccgcaggaca ggccgtccag gttcaggtcg atagtttgtg acataaaaca ctcctttaag
    35041 acagttttga ctggctgtga taaaggttaa accttccagc aaggggaagg tcaagaaatt
    35101 aataaaccag gcgggtaaaa gtccgtaaag attaaaaaat cggctcgatt tgcatcagga
    35161 ttagacattt atttctttgt tttcctgtag ttaagttgcg ggtgctaagt taaatcagga
    35221 tgcctgaaaa tcggcactgg ggtgaggaat tacctcccgc atctataaaa aggagttaac
    35281 aaaagatgtt agatgcaaac aaattacagc aggcagtgga tcaggcttac acccaatttc
    35341 actcacttaa cggcggacaa aatgccgatt acattccctt tctggcgaat gtaccaggtc
    35401 aactggcggc agtggctatc gtgacctgcg atggcaacgt ctatagtgcg ggtgacagtg
    35461 attaccgctt tgcactggaa tccatctcta aagtctgtac gttagccctt gcgttagaag
    35521 atgtcggccc gcaggcggta caggacaaaa ttggcgctga cccgaccgga ttgcccttta
    35581 actcagttat cgccttagag ttgcatggcg gcaaaccgct ttcgccactg gtaaatgctg
    35641 gcgctattgc caccaccagc ctgattaacg ctgaaaacgc cgaacaacgc tggcagcgaa
    35701 ttttacatat ccaacagcaa ctggctggtg agcaggtagc gctctctgac gaagtcaacc
    35761 agtcggaaca aacaaccaac ttccataatc gggccattgc ctggctgctg tactccgccg
    35821 gatatctcta ttgtgatgca atggaagcct gtgacgtgta cacccgtcag tgctctacgc
    35881 tcatcaatac tgttgaactg gcaacgcttg gcgcgacgct ggcggcgggt ggtgtgaatc
    35941 cgttgacgca taaacgcgtt cttcaggccg acaacgtgcc gtacattctg gccgaaatga
    36001 tgatggaagg gctgtatggt cgctccggtg actgggcgta tcgcgttggt ttaccgggca
    36061 aaagcggtgt aggtggcggt attctggcgg tcgtccctgg cgtgatggga attgccgcgt
    36121 tctcaccacc gctggacgaa gaaggcaata gtgttcgcgg tcaaaaaatg gtggcatcgg
    36181 tcgctaagca actcggctat aacgtgttta agggctgatc atgatgaaca cggaaggtaa
    36241 taacggtaac aaacccctcg gtctatggaa cgtcgtttcc atcggtattg gggcaatggt
    36301 gggggcgggg atcttcgcgc tgctggggca ggctgcgttg ctaatggaag cctcgacctg
    36361 gggcgccttt gcttttggcg gtattgtggc tatgttttcc ggttatgcct atgcgcgtct
    36421 gggggcgagc tatcccagca atggcggtat tatcgacttt tttcgtcgcg gattaggcaa
    36481 cggcgtcttt tcgctagcgc tctcgttgct atacctgttg acgctggcgg tgagcatcgc
    36541 catggttgcc cgtgcttttg gcgcttatgc cgtgcagttt ttgcatgaag gcagccagga
    36601 ggagcacctt attttgctct acgcgttggg gatcattgcg gtgatgacgc ttttcaactc
    36661 cttaagcaac catgcggtag ggcggctgga agtgatcctc gtcggcatta aaatgatgat
    36721 cctgttattg ctgattattg ccggtgtctg gtcgctgcaa ccggcgcata tttccgtctc
    36781 tgcgcccccc agctccggtg cgttcttctc ctgtattggg ataaccttcc ttgcctatgc
    36841 gggctttggc atgatggcga acgcggcgga taaagtgaaa gatccgcagg tcattatgcc
    36901 acgggcgttt ctggtggcga ttggcgttac cacgttgctt tatatctcgc tggcgctggt
    36961 tttgcttagc gatgtatcgg cattagagtt agaaaaatat gctgataccg ccgtagcgca
    37021 ggctgcttct ccgctgctcg ggcatgtggg ttatgtgatc gtcgtcatcg gcgctttact
    37081 ggcgacggct tcaggcatta acgcgaacct gttcgccgtg tttaacatca tggacaacat
    37141 gggcagcgaa cgcgaactgc cgaagctaat gaataaatcc ctgtggcggc agagtacctg
    37201 gggcaatatc atcgtcgtgg tgttgataat gctgatgacg gcggcgctaa atttagactc
    37261 actcgccagc gttgccagcg ccaccttttt gatctgctat ctggcggtgt ttgtggtggc
    37321 gatccgcctg cgtcatgata ttcacgcctc gttgccgatt cttatcgttg gtacgttggt
    37381 gatgttgttg gtgatcgttg gctttatcta cagcctgtgg tcccagggca gccgtgcgtt
    37441 gatatggatt attggtgcaa tcttactcag ccttattgtg gcaatggtca tgaagcgtaa
    37501 taaaaccgta taacacctct ctgtgcgcag tacttcctgt attattgtgg tggcggtcga
    37561 tattcgcact ggcaaaaaaa cgtgcttgaa tatctgttga aaccctttaa caaagcacag
    37621 gaggcgttgc gcgaacgatg aacatcagcg atgtagcaaa aattaccggc ctgaccagca
    37681 aagccattcg cttctatgaa gagaaggggc tggtgacgcc gccgatgcgc agcgaaaacg
    37741 gttatcgcac ctacacgcag cagcatctca acgaactgac cttactgcgc caggcacggc
    37801 aggtgggctt taacctggaa gagagcggcg agctggtgaa tctgtttaac gacccgcagc
    37861 ggcacagcgc cgacgtcaaa cggcgcacgc tggagaaggt ggcggagatc gaacgacaca
    37921 ttgaggagct gcaatccatg cgcgaccagc tgctggcact ggcgaatgcc tgccctggcg
    37981 atgacagcgc cgactgcccg attatcgaaa atctctccgg ctgctgtcat catcgggcag
    38041 ggtgattaag acgagacggc tcggatatgt agggttatcc cttctatcgc aatgacttca
    38101 acatgcgtac ctgcaccgag atcctcgctg gcgctgacag gccatgaact gtcaccgacg
    38161 cgcatatgac cgcgcccgtt gaccagcgga gattccagca caaaacgtcg gccaatcagc
    38221 tgctgcccgc gctggtttaa atgactatcg ctgtgctttt gttcgcgcac ccgccgcgac
    38281 aaccatttcc accacagcca ggcggcgagc agcgtcagga tggcaaacat taccccttgc
    38341 cactcccaac ccagcggcac cagccagacc accaggccgg taatcaccgc tgccacgccg
    38401 ctccacaaca aataaccatt tccgcccagc atctcggctg ccagcagcaa cccgccgaga
    38461 ctgagccaga aaatatgtgg atgaacgacc attaactcca tcatggctga gtccgtttgt
    38521 tggcgctgtc tttcaccagc tcggcaatcc cggcaatcga ccccatcagg ctgctggcct
    38581 ctaatggcat catcactact ttgctgttac tggaggaacc gatctgctgt aacgcttcgg
    38641 tgtatttttg cgctacgaag tagttcaccg cctgaatatc accggaggcg atagcttcag
    38701 acaccatttt ggtggcgcgg gcttctgctt cggcggaacg ttcacgcgct tcagcctgta
    38761 aaaacgccga ctgacgttcg ccttccgctt tcaggatttg cgactgtttt tcaccttcgg
    38821 ctttgaggat ttccgcctga cggatccctt ccgcttcaag aatgtaagcg cgtttggtac
    38881 gttccgcttt catctgcgcg ttcattgaag agataagctc tgccggtggg cgcacgtcgc
    38941 gaatttcaat acgggtgact ttaatccccc acgggttggt ggcttcatcg acaatacgca
    39001 gcaggcgtga gttgatgctg tcgcgctgag agagcatttc gtcaagttcc attgaaccca
    39061 acacggtacg gatgttagtc atggtcaggt tgatgatcgc cagctccaga ttgctgactt
    39121 cataggcagc gcgtggcgcg tcaatcacct gaataaaaca gactgcgtcg atggtaacgt
    39181 tggcgttatc tttcgagata acttcctggg aagggatatc gagcacttgc tccatcatat
    39241 tgatcttgcg accaatgcga tccataaacg gcaccaccag actgagcccc ggctgtaacg
    39301 ttttggtata gcgaccaaaa cgttctaccg tccattgata gccctgcggc acgattttga
    39361 cgcccgcgcc gacaatgacc agcgcgacaa aaatgagaat cgggataaag ataagcatcg
    39421 gaaaaacctc ctgttgtacc gtccataatc agcaaaattg ctgcttgatt aaacaaatta
    39481 tacctgatta ctgaaagaga gttccccctt attcctgcga aggataaact gtttttagta
    39541 aaaatcagaa aaagggaaca gcgatgcagg aaaatagtcc tttgcttcag ctacaaaacg
    39601 taggatatct ggcgggtgat acgaagattc ttaataacat caatttttcg ctgcgtgctg
    39661 gcgaatttaa gttaattacc ggtccttctg gttgtggcaa aagtacgctg ctaaaaatag
    39721 ttgcttcatt gatcagccca accagcggaa cgttactgtt tgaaggtgag gatgtcagca
    39781 cactaaaacc agaaatctac cgccagcaag tctcttactg cgcccagacg ccgacgctgt
    39841 ttggcgatac ggtatacgat aatctgatct ttccctggca gatccgtgac cagcagcctg
    39901 acccggccat ttttctcgat tttctcgaac gcttcgcttt gccggacagc attttgacga
    39961 agaatatcgc cgagctatct ggtggtgaaa aacaacgcat ctcattgatt cgtaacctgc
    40021 aatttatgcc gaaggtttta ttgctggatg aaataaccag tgcgctggat gaaagtaata
    40081 aacataacgt caatgagatg atccatcgtt atgtgcgcga gcaaaatatt gccgtgctgt
    40141 gggtgacaca cgataaagac gaaattaatc atgcggataa agtgattaca ctgcaaccgc
    40201 atgccggaga aatgcaggaa gcacgctatg aactcgcata atattactaa cgaatcatta
    40261 gcactggcat taatgctggt ggtggtggca atcttaatta gccataaaga aaaactggcg
    40321 ctggagaaag atattctatg gagcgtcggg cgagcgataa ttcagctgat tattgtcggc
    40381 tatgtgctga agtatatttt cagcgtggat gatgccagcc tgacattatt gatggtgtta
    40441 tttatctgct ttaatgcggc gtggaacgcg caaaaacgca gtaaatatat tgctaaagct
    40501 tttatctcat cgtttattgc cattacggtc ggggcgggaa ttaccctggc ggtgctgatt
    40561 ctctcagggg cgattgaatt tatcccgatg caggtgatcc ctgtcgccgg gatgattgcc
    40621 ggtaacgcca tggtagcggt ggggttgtgt tacaacaatt tagggcaacg ggtcattagc
    40681 gaacagcaac agattcagga gaaactgagt cttggcgcga cgccgaagca ggcttcagcg
    40741 atattgattc gcgacagtat tcgcgcggct ttaattccga cggtcgattc agcaaaaacg
    40801 gttggcttag tgagtttacc aggaatgatg tccgggctga tatttgccgg gattgatccg
    40861 gtgaaggcga ttaaatatca gattatggtg acctttatgc tgctctcaac cgccagcttg
    40921 tcgaccatta ttgcctgcta tttaacctat cgtaagtttt ataattcgcg ccaccagttg
    40981 gtggtgactc aattgaagaa gaaatgatgc gatgccggat gcgcagcatc gcatccggcg
    41041 ttgtggttca tgtgccgaac aacggaatat caatacaaca atgcatacag ctggcggcga
    41101 tacttcgacg ccagtgcatc acccgtaccc agcgcagcga ggatctcctg gaacgtttta
    41161 cgcgtctgac cgtctgcggc ggtgagatct ttacgcagat gcccgaacag caactccagc
    41221 gcctcttcat tgcgcccaac ctgatgcagt tgcagcgcca gttgcgtcgc cagtgcggca
    41281 tcttctggat tctccgccac ctgctgttgc aactgttgaa tttccggctt atcagccgcc
    41341 tgcttcagca gttcgatttg cgctaccagc ccctggtagc gggtgtcctg atcctgcaac
    41401 ggaatggttt tcagcaccgc ttccgcatct tcagaacggt tcagcgcaat cagcgtttct
    41461 gccagcagca ggccgatctc cccgttctga ttcgacaact gccaggcgtc tttcagcaat
    41521 ggcagggcat cggtgtaatt gccttcctgc atcagttgca tcgcctgctg cgctttcagc
    41581 tcttcttcgc gcggcagcac tttatccagc agggcgcgga tcgcctcttc cggttgcggc
    41641 ccctggaagc catctaccgg ttgcccgttc tggaacagat acacggtcgg aatcgcgcgc
    41701 agaccaaact gggcggcaat catctgctcc gcgtcgcagt ccagcttcgc cagaataaat
    41761 tgcccgttgt actgcgccgc gaggctttcc aggatcgggg ttaactgcaa acagtgctgg
    41821 ctacgttcag accaaaaata gaacagcacc ggagtggtca tcgactgttc aagaacctgt
    41881 tgcaggttag attcgttaat gttgacaata ttttctacgg acatggagtc gctctctgtt
    41941 gtcgatattt tctttgacat gggggcttaa gcgcgcgctt caactcaccc ctgcaatatt
    42001 ttgtccatca cgcgcccagg cagcaggcgc tcgagcacca ttactgccca ggtcaccagc
    42061 gttaccggat agcgcatctt cggcttctcg ctaataaaag catggcgcac tttgtcgacc
    42121 accgcttccg gtcccaacgt aaagcgggcg gcgatgccgg gattttcgac tggtttatca
    42181 ctttgcgtct ggttgacgtt gtcggtgaag cgagtacgaa tgggaccggg ttcgatcagg
    42241 ctgactttaa ttccgctgtg gcgtagctcc atgcgcagcg cgtctgacca cgcctccagc
    42301 gcatatttac tggccgcgta agcgccacga cccggcgtgg agattaatcc catcaccgat
    42361 gatgtcatca caatacgccc ttcaccgtgc ggtaacatcg cgggtaacag gcgcatggtg
    42421 agctggtgtg cgccgaaaaa gttggcggaa aactgctgtt ccatctgcgc acggctgatg
    42481 gtggaaaggg ggccatacat gccgaatccg gcattgttaa agatcccata cagacaatta
    42541 tcggtcaggg cgatcacctc gtctgctgcg cgattaacac tttctggtga atccagatcg
    42601 atcaacacgc cggtaaatcc catgttgttc atacgctcaa catcattcgg ttttcggcaa
    42661 cctgccagca catgaaaacc ctggcgtttt aattcgagcg cgctttccag gccaattcca
    42721 ctggaacatc cggtaattaa gaccgatttt tgcataactt tacctgtcag gatctccgtt
    42781 gctttatgag tcatgattta ctaaaggctg caactgcttc gccatccagt cggcaataaa
    42841 cggctgggcg tcgcggttgg gatgaatacc gtcatcctgc atccattgtg gcttgaggta
    42901 gacctcttcc ataaaaaagg gcagcagcgg aacatcaaac tctttggcga gtttggggta
    42961 aatggcgcta aaggcttcat tataacggcg accatagttt gcaggcagac gtatttgcat
    43021 taacaatggt tcagcgttgg cggctttgac atcctgcaaa atctggcgca gcgtttgctc
    43081 ggtttgctgt ggctgaaaac cacgcaaacc atcattgcgc ccagttcaac cagcacccaa
    43141 cgcggctgat gctgtttgag cagagccgga aggcgcgcca gcccttgttg cgaggtgtcg
    43201 ccgctgatgc tggcattaac taccgacgtt ttactctgcc acttatcatt caacaaggca
    43261 ggccaggccg cgctggcaga cattcgatac ccggcgctca ggctatcacc cagaatcaat
    43321 aacgtgtccg ctgcggcggc acggaaggtt aacaggacca ggaacaggaa gggcaaatgc
    43381 cagcggaaaa cattgttgaa gttcatcatc ttaagaagtc cgtcggtcag ggggagcatg
    43441 aactctccat cctcaccgga gttgagctgg ttgtcaaacg tggcgagacc atcgcactgg
    43501 tgggcgagtc gggatcgggt aagtcaacct tgctggcgat cctcgccggg cttgatgacg
    43561 gcagcagtgg cgaagtgagt ctggtgggac aaccgctaca taatatggac gaagaagcgc
    43621 gggcaaagtt gcgcgcgaag cacgtcggct ttgtttttca gttatttatg ttaattccta
    43681 cccttaacgc gctggaaaac gtcgagcttc cggctctgct gcgcggtgag agtagcgcgg
    43741 aaagtcgtaa cggggcgaaa gcgttgctcg aacagttagg gctgggtaaa cgtctggatc
    43801 atcttccggc acagctttcc ggcggtgaac agcaacgagt ggcgctggca cgagccttta
    43861 atggtcgacc tgatgtgctg tttgccgacg aacccaccgg caaccttgac cgccagacgg
    43921 gcgataaaat tgccgacctg ctgttttccc tcaaccgtga acatggcacc acgttgatta
    43981 tggtgaccca cgacctgcaa ctggcggcac gctgcgaccg ctgcttacgg ctggtgaacg
    44041 ggcagttgca ggaggaagca tgattgcacg ttggttctgg cgcgaatggc gttcgccgtc
    44101 gctattaatt gtctggctgg cgctaagcct ggcggtggcc tgcgtgctgg cgctgggcaa
    44161 tatcagcgat cgcatggaga agggcttaag ccagcaaagc cgtgagttta tggcgggcga
    44221 tcgggcgttg cgcagttcac gcgaagtgcc gcaagcgtgg ctggaggaag cgcaaaagcg
    44281 cggcctgaaa gtcggcaagc agctgacttt cgccacaatg acctttgcag gcgacacacc
    44341 gcagctggcg aacgtcaaag cggtggatga tatctacccg atgtatggcg gtctgcaaac
    44401 taatccccct ggcctgaaac cgcaggcggg cagcgtattg ctggccccac gcctgatggc
    44461 actgcttaac ctgaaaacgg gcgacaccat tgacgtgggc gatgccacct tgcggattgc
    44521 cggagaagtg attcaggaac cggattccgg ttttaacccc ttccagatgg ctccgcgtct
    44581 gatgatgaat ctggcggatg tcgataaaac cggagccgtg cagccgggga gtcgggtcac
    44641 ctggcgttat aaattcggcg gcaacgagaa ccagctcgac ggctatgaga aatggttgtt
    44701 acctcagctt aaacccgaac aacgctggta cggtctggaa caggacgaag gcgcgctggg
    44761 gcgatcgatg gaacgctcgc aacagttcct gctgctttcg gcgcttctga ccttgctgct
    44821 ggcagtggca gcggtggcgg tagcgatgaa tcattactgt cgcagtcgct acgatctggt
    44881 ggcgatcctc aaaacgctgg gggcagggcg agcgcaactg cgtaagctaa tcgtcggtca
    44941 gtggttgatg gtgctgacgc tttcagccgt taccggtggg gccataggcc tgttgttcga
    45001 aaacgtgttg atggtgctgc tcaagcccgt tctgcctgct gcactaccgc cagccagcct
    45061 ctggccgtgg ctgtgggcgc ttggcaccat gacggtcatc tcgctgctgg tggggctacg
    45121 accataccgc ttgttgctgg caacgcagcc tttacgcgta ttacgtaatg atgtggtcgc
    45181 gaacgtctgg ccgctgaagt tttatctgcc gattgtcagt gtggtggttg tgctgctgct
    45241 cgccggatta atgggtggca gcatgctgct ttgggcggtg ctggcgggcg cggtagtact
    45301 ggctttgctg tgcggtgtgc tgggctggat gctgctgaat gtacttcgcc gcatgacgct
    45361 gaaatcgctg cctctgcgcc tggcggttag ccgcctgtta cgtcagccgt ggtcaacgtt
    45421 aagccagctt tcggcatttt cgctctcctt tatgctgctg gcactgctgc tggtgctgcg
    45481 tggcgatctg ctcgaccgct ggcagcagca gctaccgcca gaaagcccga actacttttt
    45541 aattaacatc gccacagaac aggtagcacc gctaaaagcg ttcctcgcgg aacatcatat
    45601 agtcccggaa tcattttatc cggtagtgcg ggcgcggctg acggcgatta atgataagcc
    45661 gacagaaggt aatgaagatg aggcgcttaa tcgcgaactc aatcttacct ggcaaaatac
    45721 gcggcccgat cataatccga ttgtcgccgg taactggccg ccaaaagccg atgaagtgtc
    45781 gatggaagag gggctggcga aacgcttaaa cgttgccctc ggtgataccg tgacttttat
    45841 gggcgatacc caggagttcc gcgctaaagt gaccagcctg cgcaaagtgg actgggaaag
    45901 tctgcggcct aatttctatt ttattttccc tgaaggggca ttagacgggc aaccgcagag
    45961 ctggcttacc agtttccgct gggagaatgg caacggcatg ttgacgcaac tcaaccgcca
    46021 gttcccgacc attagcctgt tagacattgg cgcgatttta aaacaggtcg gtcaggtgct
    46081 ggagcaggta agtcgggcgc tggaggtgat ggtggtgctg gtcaccgcct gcggtatgtt
    46141 gctgttgctg gcacaggtgc aggtgggaat gcgccagcgt catcaggagc tggtggtatg
    46201 gcgcacactc ggtgcgggga aaaaactgct gcgcacgaca ttgtggtgtg atgttcgcca
    46261 tgcttgggtt tgtttccggc ctggtggccg caattggtgc ggaaacggca ctggcagtgt
    46321 tgcaggcgaa agtgtttgat ttcccgtggg agccagactg gcgattgtgg attgttctgc
    46381 cgtgcagcgg tgcgctgctg ctgtcgcttt tcggcggctg gctgggtgcg cgactggtta
    46441 agggtaaggc gctgttcagg cagtttgtgg ggtgatgaca ggtgttagat tgcctgatgc
    46501 gctacgctta tcaggcctac gaagagcgtg caatatattg attttgcaag attgtgtagg
    46561 ccggataagg cgtttacgcc gcatctggca tgaacagtgt gcattttggc aataaatatg
    46621 aaaccaactg tcatgtcggt ttttttaaag gccgaaggag gtaaaccttc ggccttagct
    46681 acgtttcctc agttatttaa taaactgacg gatcagttcc agcagcgcga ttagcgcctt
    46741 aagaacgatg agaactttat cgagaaaagt tttcatccgt tgctccctta agggagaaag
    46801 acaatcacag cttaccctta cgttgcctat aactatgatt ttcttcatgt gctagatgag
    46861 gactgtctct ctccaaatta gagcaccaga atcagcacca cctgtatcct gaccaggact
    46921 taaaagtact tttccgcact gcggtaatcc cttgaggcag tatcactact agcgtcggga
    46981 ttgtatcgcc aacatagctg tttgaatatg taggcatcct gacttataat tcacttgcta
    47041 gatgaggact gtttaatttc tgcccggaac caccaagtct tgggcggaaa aaataaaaaa
    47101 accgactgtc atgtcggttt ttttatatct gtattctggt gcaaccattt tgttctcacg
    47161 acaatcgccg tttcagaata ccgcccatca ctgcgcctta acccataccg ccacttccgc
    47221 ccactcaccg cgaaagacaa ctttttccgc ttttttctca agctgatagc gatacattgg
    47281 gtcgtaatat tctttaagta acggcaccag ccaggccaga tgaccgtcgg tgctgccggt
    47341 ggtgagttgc gttgtcagcg ccgtatccag ctgggcagcg agttcgttat agcgctgtaa
    47401 ccccagccga cgcttaatcg ccgaaagtcc gtgatgcagg tattcgcaat attcctgcca
    47461 gccctgttcg tcgccataag cgtgggtaaa atcatgatgc atacgcaaga aatactcttc
    47521 attcaggcgt tcaagacgga tctcaaacgg atcttctacc accgcaatcg ccgcctgagt
    47581 cattcgctcg cgcagacatt ccggcaggtg attcgaaccg atcatccggc tttcatcttc
    47641 cagcacccac aggcgcaaat cctgacgggc gtcggttttt agcatttcgg cagccagcag
    47701 gttttcaaaa ctcgcctggc taagttgtgg ttgtaacgtg cgaccaaacg ccgaaccgcg
    47761 atgacgcgcc agcccttcca gatcaacacc gtttggctgt tgttgcacta atagcgtttt
    47821 accgctgccg gtacaaccgc caatcagcac tatcggtttt tgtgccagtt caatggtcgc
    47881 ctgaatcgcg gtctggcgca gtgccttata accgccttcc accagcggat aatcaatccc
    47941 cgcttcatgc agccagcgtt gcacaatatg tgaacgttga ccgccacggg cgcagcagag
    48001 aataccttgc ggattttgca ggcacgctgc ccgccaggcg tacatacgct gctgacgaat
    48061 ttcacccgcc accagtttat gccccagcgc cagcgctgcg tctgagcctt gctgtttata
    48121 gcaggtgcca acggaggcgc gttcatcgtt atccattaac ggcagattga tagcggcggg
    48181 cattgcgcct tgctcaaact cgataggggc gcgaacatca ataatgggcg tatcagcaat
    48241 cagcagggca cgatagtcct gttccgtgtg tctctcttgc atagttaaaa gtgaacctca
    48301 aatcagcttg cgcgctattt tacgcgccaa cgcgcaagga aacttgattt ttaactgcgt
    48361 ggggtgccga aaatttctaa aaatccgctg atttccggcc tgtgttgggt aaacaaggtc
    48421 acaatatctt ctaccgcttt gcagctgcca aatttgcgcc atgccagact caatggcgaa
    48481 ggagggcgca tcgttgggat tacccggctg accagttgtt gattatcgat cattgactgg
    48541 caaagtgatt ttggcaaaaa accaatgcca acacccgcca gatgggcggc gattttcgtt
    48601 tccatatcgg gaacaataat ctctttttgc cccggcaatc gccaggcgac gcgtttggtt
    48661 aaggtgccgg cgctgtcttc aatattgacc gccggaaagc gacgcaactg cgcttctgtt
    48721 agcggctctt caacgttcgc cagcggatga tccgccgcca tgacaaatcg ccattgcacc
    48781 gatcctaagg gatcaagact aaaggtattt gccagcgcct cagttcccgt gaccccgata
    48841 gccagcgaaa aaccttcgta caatagcgag tcccagacgc ccatatagat ttgtcgggag
    48901 atgtgaaact gggtaaaggg gtaacgctca ttcagccacg ccagcaactg ggcgacggcc
    48961 tgggggttat agagcaggtt gttgatgaca atattcacct ggcgttccac gccatcattc
    49021 acctgttgca gctcgctggg catactttcc agccagctca gccagtctct ggcctgggaa
    49081 agtagatgct cgccagccgc tgtcaacgtt acgctacgag tcgtacggaa aaacagcgct
    49141 actccggtat tctcttccag aagtttaatg cgataactga tcgtcgccgt ggttttacat
    49201 aatcgttctg ccgcttttga aaaacttcct gtttcagcaa ccgcaatgaa agtccgcaag
    49261 gtttctggat cgaacatctt caggtatccc cttttaaatc cgcaagttgc gtgattttct
    49321 tatcctctga tttatcagta tttttacatg ataatcctgt tcaatttgtg gactaaatct
    49381 agttttggaa aaacattcca acttttgtat taatgttgtt ctcttaaggt tttagattgc
    49441 ctgttattga aaccaagctg accggtcggc ggtggttgaa cggaattatg ttacaaggac
    49501 aaaaagatga aacttcaggt attaccgtta agtcaggaag cctttagtgc ttatggcgac
    49561 gtaatcgaaa cgcagcaacg ggattttttc catattaaca atggcctggt ggagcgttac
    49621 cacgatttgg cgctggttga gattcttgag caagaccgta cgcttatcag cattaaccgc
    49681 gcgcaaccgg cgaatctgcc gctgaccatt cacgaactcg aacgtcatcc gctgggcatt
    49741 caggccttta tcccaatgaa aggtgaagtg tttgtggtgg tcgtggcgtt aggcgacgac
    49801 aaaccagacc tgtcaacgct gcgggcgttt atcaccaacg gtgaacaggg ggtgaattac
    49861 catcgtaacg tctggcatca cccacttttc gcctggcagc gcgtcaccga ttttctgacc
    49921 atcgatcgcg gcggcagtga caactgtgat gttgaaagta ttcctgaaca ggaactctgt
    49981 tttgcgtgac gcctgcaacc gacttgcata agataaacta attgttcatt gtttatgctc
    50041 acttgtaggt cggagttaac gtaggtatga cggaagttag acggcgcggc aggccaggac
    50101 aggcggagcc tgtggcacag aagggcgcac aggcgttaga gcggggaatt gcgattctgc
    50161 aatatctgga aaaaagtggg ggaagttcgt cggttagcga tatttctctc aatctggatt
    50221 tgccgctctc cacgaccttt cgcttgctga aggttttaca agcagcggat tttgtctatc
    50281 aggacagtca attaggctgg tggcatatag gattaggtgt ctttaacgtc ggtgcggcgt
    50341 acatccataa ccgcgatgtc ctctccgtcg ccgggccgtt tatgcgccgc ctgatgttac
    50401 tttccggcga aacggtcaat gtcgcgatcc gtaacggcaa tgaagcggta ttaattggtc
    50461 agttagagtg taaatcgatg gtcaggatgt gtgcgccact gggcagtcgt ctgccgctgc
    50521 atgcttccgg tgcgggcaaa gcgctgcttt atccgctggc ggaagaggag ttgatgagca
    50581 tcattctgca aaccggtttg cagcagttta cgccaactac gcttgtggat atgcccacct
    50641 tgctgaagga cctggaacaa gcgcgtgaac tgggctatac cgtagataaa gaagagcatg
    50701 ttgtaggtct gaattgcata gcttcagcaa tttacgatga tgtcggtaga gttgttgccg
    50761 ctatctccat ctccgggcct tcatcaagac tgacagaaga tcgttttgtc agtcagggtg
    50821 agctggtcag agacaccgcc cgcgatatca gcacggcgtt gggactgaaa gcacatccat
    50881 agtgtctgtc gcatcccgct ctgcggagcg ggtttttttg acaaaatttg aaagttggaa
    50941 aatttttcca actaatagag gtaggaataa aatggcaaaa atgagagccg ttgacgcggc
    51001 aatgtatgtg ctggagaaag aaggtatcac caccgccttc ggcgttccgg gagctgcaat
    51061 taatccgttc tactcagcga tgcgtaagca cggcggtatt cgtcacattc tggcgcgtca
    51121 tgtggaaggt gtttcgcaca tggcggaagg ttatacccgc gcaacggcag ggaatatcgg
    51181 cgtatgtctg gggacttccg gtcctgcggg cacggacatg atcaccgcgc tctattccgc
    51241 ttctgctgat tccattccta ttctgtgcat taccggccag gcaccgcgcg cccgtctgca
    51301 taaagaagat tttcaggccg tagatattga agcaattgct aaaccggtca gcaaaatggc
    51361 ggttacagtt cgtgaagcgg cgctggtgcc tcgcgtgctg caacaggcat ttcacctgat
    51421 gcgttctggt cgtccgggtc cggtactggt ggatttaccg ttcgacgttc aggttgcgga
    51481 aatcgagttt gatcctgaca tgtacgaacc gctgccggtc tacaaacctg ctgccagccg
    51541 tatgcagatc gaaaaagctg tagaaatgtt aatccaggcc gaacgtccgg tgattgttgc
    51601 cgggggcggg gtaatcaatg ctgacgcagc tgcactgtta caacagtttg ctgaactgac
    51661 cagcgttccg gtgatcccaa cgctgatggg ctggggctgt atcccggacg atcatgaact
    51721 gatggccggg atggtcggtc tgcaaaccgc gcatcgttac ggtaacgcaa cgttgctggc
    51781 gtccgacatg gtgtttggta tcggtaaccg ttttgctaac cgtcataccg gttcggtaga
    51841 gaaatacacc gaagggcgca aaatcgttca tattgatatt gagccgacgc aaattggtcg
    51901 cgtgctgtgt ccggatctcg gcattgtctc tgatgctaaa gcggcgctga cactgctggt
    51961 tgaagtggcg caggagatgc aaaaagcggg tcgtctgccg tgtcgtaaag aatgggtcgc
    52021 cgactgccag cagcgtaaac gcactttgct gcgcaaaacc cacttcgaca acgtgccggt
    52081 gaaaccgcag cgcgtgtatg aagagatgaa caaagccttt ggtcgcgatg tttgttatgt
    52141 caccactatt ggtctgtcac aaatcgctgc ggcacaaatg ctgcatgtct ttaaagaccg
    52201 ccactggatc aactgtggtc aggctggtcc gttaggctgg acgattccgg cggcgctggg
    52261 gggttgtgcc gctgatccga aacgcaatgt ggtggcgatt tctggcgact ttgacttcca
    52321 gttcctgatt gaagagttag ccgttggcgc gcagttcaac attccgtaca tccatgtgct
    52381 ggtgaataac gcctatctcg gcctgattcg tcagtcacaa cgcgcttttg acatggacta
    52441 ctgcgtgcaa ctcgctttcg agaatatcaa ctccagtgaa gtgaatggct acggtgttga
    52501 ccacgtaaaa gtagcggaag gtttaggttg taaagctatt cgggtcttca aaccggaaga
    52561 tattgcgcca gcctttgaac aggcgaaagc cttaatggcg caatatcggg taccggtagt
    52621 cgtggaagtt attctcgagc gtgtgaccaa tatttcgatg ggcagcgaac tggataacgt
    52681 catggaattt gaagatatcg ccgataacgc agcggatgca ccgactgaaa cctgcttcat
    52741 gcactatgaa taagggagat aaatgatgtt acgtttctct gctaatttat cgatgttatt
    52801 tggtgaatat gattttctcg cccgttttga gaaagctgcg cagtgtggtt ttcgcggcgt
    52861 tgaatttatg tttccttatg actacgacat tgaagaatta aaacaggtac tggcgagtaa
    52921 taaactcgaa catacgctgc acaatttacc ggcaggtgac tgggcggcgg gcgagcgcgg
    52981 tattgcctgt attcctggcc gtgaagaaga gtttcgggat ggcgtagcag cagcgattc
//
LOCUS       DAJTQQ010000019        53036 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000019 DAJTQQ010000000
VERSION     DAJTQQ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53036
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.019"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..724)
                     /gene="cra"
                     /locus_tag="OO309_001228"
     CDS             complement(<1..724)
                     /gene="cra"
                     /locus_tag="OO309_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR5846432.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQA"
     gene            complement(806..892)
                     /locus_tag="OO309_001229"
     CDS             complement(806..892)
                     /locus_tag="OO309_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR5846433.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(904..1395)
                     /gene="ilvN"
                     /locus_tag="OO309_001230"
     CDS             complement(904..1395)
                     /gene="ilvN"
                     /locus_tag="OO309_001230"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5846434.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1398..3122)
                     /gene="ilvI"
                     /locus_tag="OO309_001231"
     CDS             complement(1398..3122)
                     /gene="ilvI"
                     /locus_tag="OO309_001231"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR5846435.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPRATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3241..3285
                     /locus_tag="OO309_001232"
     CDS             3241..3285
                     /locus_tag="OO309_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846436.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3440..4384)
                     /gene="leuO"
                     /locus_tag="OO309_001233"
     CDS             complement(3440..4384)
                     /gene="leuO"
                     /locus_tag="OO309_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR5846437.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5043..5129
                     /gene="leuL"
                     /locus_tag="OO309_001234"
     CDS             5043..5129
                     /gene="leuL"
                     /locus_tag="OO309_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR5846438.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5222..6793
                     /gene="leuA"
                     /locus_tag="OO309_001235"
     CDS             5222..6793
                     /gene="leuA"
                     /locus_tag="OO309_001235"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR5846439.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            6793..7884
                     /gene="leuB"
                     /locus_tag="OO309_001236"
     CDS             6793..7884
                     /gene="leuB"
                     /locus_tag="OO309_001236"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR5846440.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            7887..9287
                     /gene="leuC"
                     /locus_tag="OO309_001237"
     CDS             7887..9287
                     /gene="leuC"
                     /locus_tag="OO309_001237"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR5846441.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9298..9903
                     /gene="leuD"
                     /locus_tag="OO309_001238"
     CDS             9298..9903
                     /gene="leuD"
                     /locus_tag="OO309_001238"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR5846442.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(9952..11130)
                     /gene="setA"
                     /locus_tag="OO309_001239"
     CDS             complement(9952..11130)
                     /gene="setA"
                     /locus_tag="OO309_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR5846443.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11232..11363)
                     /gene="sgrT"
                     /locus_tag="OO309_001240"
     CDS             complement(11232..11363)
                     /gene="sgrT"
                     /locus_tag="OO309_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR5846444.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11452..13107
                     /gene="sgrR"
                     /locus_tag="OO309_001241"
     CDS             11452..13107
                     /gene="sgrR"
                     /locus_tag="OO309_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR5846445.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13141..13240
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13271..14254
                     /gene="thiB"
                     /locus_tag="OO309_001242"
     CDS             13271..14254
                     /gene="thiB"
                     /locus_tag="OO309_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR5846446.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14230..15840
                     /gene="thiP"
                     /locus_tag="OO309_001243"
     CDS             14230..15840
                     /gene="thiP"
                     /locus_tag="OO309_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR5846447.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            15824..16522
                     /gene="thiQ"
                     /locus_tag="OO309_001244"
     CDS             15824..16522
                     /gene="thiQ"
                     /locus_tag="OO309_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR5846448.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16636..17400)
                     /locus_tag="OO309_001245"
     CDS             complement(16636..17400)
                     /locus_tag="OO309_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5846449.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17486..18364)
                     /gene="araC"
                     /locus_tag="OO309_001246"
     CDS             complement(17486..18364)
                     /gene="araC"
                     /locus_tag="OO309_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR5846450.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            18703..20403
                     /gene="araB"
                     /locus_tag="OO309_001247"
     CDS             18703..20403
                     /gene="araB"
                     /locus_tag="OO309_001247"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR5846451.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20414..21916
                     /gene="araA"
                     /locus_tag="OO309_001248"
     CDS             20414..21916
                     /gene="araA"
                     /locus_tag="OO309_001248"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR5846452.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22116..22811
                     /gene="araD"
                     /locus_tag="OO309_001249"
     CDS             22116..22811
                     /gene="araD"
                     /locus_tag="OO309_001249"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR5846453.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            22886..25237
                     /gene="polB"
                     /locus_tag="OO309_001250"
     CDS             22886..25237
                     /gene="polB"
                     /locus_tag="OO309_001250"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR5846454.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25402..28308
                     /gene="rapA"
                     /locus_tag="OO309_001251"
     CDS             25402..28308
                     /gene="rapA"
                     /locus_tag="OO309_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR5846455.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28320..28979
                     /gene="rluA"
                     /locus_tag="OO309_001252"
     CDS             28320..28979
                     /gene="rluA"
                     /locus_tag="OO309_001252"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR5846456.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29096..29911)
                     /gene="djlA"
                     /locus_tag="OO309_001253"
     CDS             complement(29096..29911)
                     /gene="djlA"
                     /locus_tag="OO309_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR5846457.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30166..32520
                     /gene="lptD"
                     /locus_tag="OO309_001254"
     CDS             30166..32520
                     /gene="lptD"
                     /locus_tag="OO309_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR5846458.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32573..33859
                     /gene="surA"
                     /locus_tag="OO309_001255"
     CDS             32573..33859
                     /gene="surA"
                     /locus_tag="OO309_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR5846459.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            33859..34848
                     /gene="pdxA"
                     /locus_tag="OO309_001256"
     CDS             33859..34848
                     /gene="pdxA"
                     /locus_tag="OO309_001256"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR5846460.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            34845..35666
                     /gene="rsmA"
                     /locus_tag="OO309_001257"
     CDS             34845..35666
                     /gene="rsmA"
                     /locus_tag="OO309_001257"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR5846461.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            35669..36046
                     /gene="apaG"
                     /locus_tag="OO309_001258"
     CDS             35669..36046
                     /gene="apaG"
                     /locus_tag="OO309_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR5846462.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36053..36895
                     /gene="apaH"
                     /locus_tag="OO309_001259"
     CDS             36053..36895
                     /gene="apaH"
                     /locus_tag="OO309_001259"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR5846463.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(36973..37452)
                     /gene="folA"
                     /locus_tag="OO309_001260"
     CDS             complement(36973..37452)
                     /gene="folA"
                     /locus_tag="OO309_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR5846464.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37643..39505)
                     /gene="kefC"
                     /locus_tag="OO309_001261"
     CDS             complement(37643..39505)
                     /gene="kefC"
                     /locus_tag="OO309_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR5846465.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWVKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39498..40028)
                     /gene="kefF"
                     /locus_tag="OO309_001262"
     CDS             complement(39498..40028)
                     /gene="kefF"
                     /locus_tag="OO309_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR5846466.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40076..41470)
                     /locus_tag="OO309_001263"
     CDS             complement(40076..41470)
                     /locus_tag="OO309_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5846467.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41529..41816)
                     /gene="fixX"
                     /locus_tag="OO309_001264"
     CDS             complement(41529..41816)
                     /gene="fixX"
                     /locus_tag="OO309_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR5846468.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(41813..43099)
                     /locus_tag="OO309_001265"
     CDS             complement(41813..43099)
                     /locus_tag="OO309_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5846469.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43150..44091)
                     /locus_tag="OO309_001266"
                     /pseudo
     CDS             complement(43150..44091)
                     /locus_tag="OO309_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44106..44876)
                     /locus_tag="OO309_001267"
     CDS             complement(44106..44876)
                     /locus_tag="OO309_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR5846470.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45232..46746
                     /gene="caiT"
                     /locus_tag="OO309_001268"
     CDS             45232..46746
                     /gene="caiT"
                     /locus_tag="OO309_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR5846471.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            46777..47919
                     /gene="caiA"
                     /locus_tag="OO309_001269"
     CDS             46777..47919
                     /gene="caiA"
                     /locus_tag="OO309_001269"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR5846472.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48048..49265
                     /gene="caiB"
                     /locus_tag="OO309_001270"
     CDS             48048..49265
                     /gene="caiB"
                     /locus_tag="OO309_001270"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCR5846473.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49339..50892
                     /gene="caiC"
                     /locus_tag="OO309_001271"
     CDS             49339..50892
                     /gene="caiC"
                     /locus_tag="OO309_001271"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR5846474.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51001..51786
                     /gene="caiD"
                     /locus_tag="OO309_001272"
     CDS             51001..51786
                     /gene="caiD"
                     /locus_tag="OO309_001272"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR5846475.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            51792..52382
                     /gene="caiE"
                     /locus_tag="OO309_001273"
     CDS             51792..52382
                     /gene="caiE"
                     /locus_tag="OO309_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR5846476.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52468..52863)
                     /gene="caiF"
                     /locus_tag="OO309_001274"
     CDS             complement(52468..52863)
                     /gene="caiF"
                     /locus_tag="OO309_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR5846477.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
BASE COUNT        12964 a        14329 c        13739 g        12004 t
ORIGIN      
        1 gcgcctgcgg catcggatgc gtttccagcc atttttcgaa taactgggca gccgcctccc
       61 gctcatagct gttggcatac aggaaatgca cttcgcgcgg atcatctttc caagcagtac
      121 ggaaaccttg ttcacgcagg aagctgacag aaagctccgg cagcgcgcca agataaagca
      181 ccgtctcggc gggaaactta cgtaactctt ccgccagcat ttcggcatca tcctgatcgg
      241 caccaaccac gctggtgaaa tgttcacgat cgagggcgcg gtccagcgcg acaatcggga
      301 acgggtcgtt agcccagcgt tgataaaaag gatgctcagg tggcaacgac gtcgaaacaa
      361 taatggcatc aacctgacgc tgtaaaaggt gctcaatgca gcgcatttcg ttgtctggct
      421 gatcttctga gcaggcaatc agcagttgat aaccccgttg ccgcgcctgg cgttcaagat
      481 agttagcgat gcgggtatag ctggtgttct ccagatcggg gatcacaaga ccaatagaac
      541 gtgtgcgtcc agcacgaagc ccagctgcca cggcgttcgg gtggtaattg tgctcacgca
      601 ccacagccat gactttttca acggttttgt cgctcacacg gtattgcttc gctttgccgt
      661 taataacata gcttgcagtg gtccgcgaca ctcccgccag ccgagcgatt tcatccagtt
      721 tcacaattgc cccttgcgta aaatgtaaaa accatagcca ttgtactggt atgggttaaa
      781 ttcattaaca tctaagcgca gaaaattaac tgcggcaact gcttttattc caggttccca
      841 ctgatttcgc aaaaaaagcc caacgtgaca tgttgggctg caaattgcgc actgagatca
      901 ttctcaacgc attattttat cgccgcgcga aagtccgacc acaccagagc gagcaacttc
      961 cacaattttc gccacatcgc gaatcgatgc taaaaatgca tcaagcttat cgctggtgcc
     1021 tgctaattga acggtataaa gcgagggggt gacatcgata atttgcccac ggaatatttc
     1081 cgtattacgt ttcacttcgt cacgcccgta accgctggcc tgaattttca ccagcatgat
     1141 ttcccgctca acatgcgcgc cctgccccaa ctcactcacg cgcaagacat cgaccagctt
     1201 gtgtaattgc ttttcgatct gctcaagtac tttttcatcg cccacggtct ggatggtcat
     1261 acgcgataat gtcggatcgt cggttggcgc aacggtcagg ctttcaatgt tgtagcctcg
     1321 ctgggaaaaa aggccaatca cgcgggataa cgcgcctgat tcattttcga gtaagactga
     1381 taatatccgg cgcataatca ggttctctcc gttttgctta accacatttc atccattccg
     1441 cccccgcgaa tctgcatcgg gtagacgtgc tcactgccat cgacggtaac atcaacaaac
     1501 accaggcgat tattgcgcac ctgttccagc gcctcgctaa gtttgctttc cagctcatgc
     1561 ggatgagaga tctggatccc gacgtgacca taggcttccg ccagacggac gaaatcgggt
     1621 agtgattgca tataagattg tgaatgacgg ccggaataga tcatgtcctg ccactgtttc
     1681 accatcccca gatagcggtt attgagattc accaccagta cgggcaactc gtattgcaac
     1741 gcggtagaca gttcctggat gttcatctga atactgccgt cgccagtgac gcaaaccacg
     1801 gtttcttctg gcaacgccat tttgacgccc agtgccgcag gtaaaccaaa acccatcgtg
     1861 ccgagaccgc cggaattgat ccagcgacgc ggtttgtcga atggataata aagcgcagca
     1921 aacatctggt gctgcccgac atcggacgtc acgtaagcgt ccccattagt caaccgccaa
     1981 agagtctcga tcaccgcctg cggtttaatc ttttcactgt gagtgtcata tttcaggcac
     2041 tgaagagcgc gccactgttc aatctgctgc caccagtcgc ggatctcatc cagcggttga
     2101 tgggcggatt cttgcgacag gagttcaagc atttgttcga ggacctggcg agcatccccc
     2161 acaatcggga tatccgcagt cacggtttta gaaatggaag taggatcaat atcgatatgc
     2221 agaacagtgg catttgggca gtactttgcc agattgttcg tcgttcggtc atcaaatcgt
     2281 accccgacgg cgaaaatcac atccgcgtta tgcatcgtca tattggcttc gtaggtaccg
     2341 tgcattccca gcatgcccag tgcctgacga tgcgttgccg gaaacgcccc cattcccatc
     2401 aatgaggaaa caacgggcag attcaacgcc tccaccgttt ctttcaactg ctgatggcag
     2461 cccgccatga ttgccccacc gcctacgtag acaaccggtt tttttgccgc taccagcgtt
     2521 tgcagagcac gcttaatttg ccctttatgt ccggtagtag tgggattgta agaacgcata
     2581 ctgaccgact cagggcagac atagggtaat ttgttcgccg gattaagaat atctttcggt
     2641 aaatcaacga ccactggtcc tgggcgacca cttgccgcca gccagaaagc ctttttcagc
     2701 acctgcggaa tgtcttccgt ttgcttaacc agaaaactgt gtttaaccac cggtcgcgaa
     2761 atccccacca tgtcgcactc ctgaaaggca tcgtaaccta tcaacgaggt cgctacctgc
     2821 ccggaaagga caactaatgg aatggaatcc atataagcgg tggcgatgcc ggtaatcgca
     2881 ttggtcgccc ttggacccga cgttaccagc acgacgccga cttccccggt cgcacgcgcc
     2941 aggccatcgg ccatatgcac cgccgcctgc tcatgacgaa ctaatacatg atcaatacca
     3001 cccacggtat gcaatgcatc gtaaatatca aggactgcgc ctccgggata accgaatact
     3061 tgtttaacgc cctgatcgat aagcgatcgg acgaccatct cggctccaga caacatctcc
     3121 atggcctgcc tcactgtttg acggaaaaaa tgtgtaaaac acattaaccg tccgatcttg
     3181 cttaagcaat tggcagccag agacataaac gcagggtaat aagaataaac aggaggaaag
     3241 gtgaaaagaa acgataaaat cctccattac gaaggattaa attgataata aatcggctga
     3301 atcccacaac ttacagcaat ttctctattt ttttaattaa tcctaaaata gagagtccac
     3361 acattcagaa taaaattcta cattcaataa aaaatgcaga ataaaccaga cattcatgtc
     3421 tggcctattc tgcattcagt tagcgtttgc aaattgagac taattgttct tccatccact
     3481 gatggccttt atcgcgcccg gcagcttcat gccaagagag ataacaggtt ctgctgtttt
     3541 gttttaacgg cagcggtaat acctgtaatt ctaaggattc agcgaactct tcagccagcc
     3601 aacgcggcgc aatagcgacc aaatgcgttt gcgacaccac gctaagtacg ctcatcattg
     3661 ccatgccctg atacgcgata ctggcttgct tatctaccgt gtcataccaa ggttgactaa
     3721 atgacgcgaa acgatcgagc gaaaccgccg catgttgttc gttataaaca tcatgtttca
     3781 gtaacgggcc tttaattgtt ggatgatttt tgctggctac cagcaccatt tcatctttaa
     3841 ataatggcac gctggtaaat tcaggacgat ggaagtcttc ataactaatc acaaactccg
     3901 tttcctgata acgcagctga tgttcagtgt tctgatttaa tgaagacttg aacataacat
     3961 gtatatttgg cgcaatctgc tcaatgtgat tataaatctg cgaggtcaga atgctgtcta
     4021 acgggctgca aacacaaaga tgaaatacac gttcactgct cgcgggttca aaacctgaac
     4081 caggcaattc attttgtact agttgcaatg cctggcgaac tgaaccaaaa agttgaaatg
     4141 cgcgagcagt cggttgaata ccacggccat aacgaacaaa aagctcgtca ttaaacatca
     4201 ccttcaggcg tgcaacagcg ttactgaccg caggttgcga catacccaga acatgagcgg
     4261 cacgggtaat gttttgctcc tgcatcacgg catcgaaaac ggttaataag ttgagatcga
     4321 ccatgcgtag ctgtggcttg cttaactccg ccgtctctga atgatctgtt tgtacctctg
     4381 gcatacttaa ctccactgtc acacttaact ccctttccct tattggaatg cagagaaata
     4441 atcctgaaga atatgattta tcatgcatat aaaataagaa aaagcaaaat gagtaaaatt
     4501 cggaaaacat aaagatacgg gttctgataa aaacagaatc cattgccgtg gaatgagtca
     4561 tttaccacag cataataatc cataatgttt tgttgcgaaa acaatctaat cataactacc
     4621 gcgaatactc aatcatctac aaaatggatt aaatgtgctt atttaataat taatttatat
     4681 atttaatgca ttaattctta acattaattg ttcaataata ttcaccaaat caatatcaaa
     4741 aaaaatcgca aaacatataa ttcaatacaa atcatcagga taggttttgc aacgcgtgca
     4801 ttttgtcccc tttttcctcg ttgattagat gcaaaaattt atgctgaaat atgtcaaccg
     4861 atgaaaagcg tcggtagtta agcagaaatt aatatcgctt actttaacca ccgtagcaca
     4921 attagctaat tttacggatg cagaactcac gctggcggga cgtttttatt gcgtcagggt
     4981 tgacatccgt ttttgtatcc agtaactcta aaagcatatc gcattcatct ggagctgatt
     5041 taatgactca catcgttcgc tttatcggtc tactactact aaacgcatct tctttgcgcg
     5101 gtagacgagt gagcggcatc cagcattaag ccagcacgca gtcaaacaaa aaacccgcgc
     5161 cattgcgcgg gtttttttat gcccgaagcg aggcgctcta aaagagacaa ggacccaaac
     5221 catgagccag caagtcatta ttttcgatac cacattgcgc gacggtgaac aggcgttaca
     5281 ggcaagcttg agtgtgaaag aaaaactgca aattgcgctg gcccttgagc gtatgggtgt
     5341 tgacgtgatg gaagtcggtt tccccgtctc ttcgccgggc gattttgaat cggtgcaaac
     5401 catcgcccgc caggttaaaa acagccgcgt atgtgcgtta gctcgctgcg tggaaaaaga
     5461 tatcgacgtg gcggccgaat ccctgaaagt cgccgaagcc ttccgtattc atacctttat
     5521 tgctacctcc ccaatgcaca tcgccaccaa gctgcgcagc accctagacg aggtgatcga
     5581 acgcgctatc tatatggtga aacgcgcccg taattacacc gatgatgttg aattttcttg
     5641 cgaagatgcc gggcgtacac ccattgccga tctggcgcga gtggtcgaag cggcgattaa
     5701 tgccggtgcc accaccatca acattccgga caccgtgggc tacaccatgc cgtttgagtt
     5761 cgccggaatc atcagcggcc tgtatgaacg cgtacctaac atcgacaaag ccattatttc
     5821 cgtacatacc cacgacgatt taggcctggc ggtcggcaac tcactggcgg cggtacatgc
     5881 cggtgcacgc caggtggaag gtgcaatgaa cgggatcggc gagcgtgccg gtaactgttc
     5941 gctggaagaa gtcatcatgg cgattaaagt tcgtaaggat attctcaacg tccacaccgc
     6001 cattaatcac caagagatat ggcgcaccag ccagttagtt agccagattt gtaatatgcc
     6061 gatcccggca aacaaagcca ttgttggcag cggcgcattc gctcactcct ccggtatcca
     6121 ccaggatggc gtgctgaaaa accgcgaaaa ctacgaaatc atgacaccag aatctattgg
     6181 tctgaaccaa atccagctga atctgacctc tcgttcgggg cgtgcggcgg tgaaacatcg
     6241 catggatgag atggggtata aagaaagtga atataattta gacaatttgt acgatgcttt
     6301 cctgaagctg gcggacaaaa aaggtcaggt gtttgattac gatctggagg cgctggcctt
     6361 catcggtaag cagcaagaag agccggagca tttccgtctg gattacttca gcgtgcagtc
     6421 tggctctaac gatatcgcca ccgccgccgt caaactggcc tgtggcgaag aagtcaaagc
     6481 agaagccgcc aacggtaacg gtccggtcga tgccgtctat caggcaatta accgcatcac
     6541 tgactataac gtcgaactgg tgaaatacag cctgaccgcc aaaggtcacg gtaaagatgc
     6601 gctgggtcag gtggatattg tcaccaacta caacggtcgc cgcttccacg gcgtcggcct
     6661 ggccaccgat attgtcgagt cctccgccaa agccatggtg cacgtactga acaatatctg
     6721 gcgtgccgca gaagtcgaaa aagagttgca acgcaaaact caacacaacg aaaacaacaa
     6781 ggaaaccgtg tgatgtcgaa gaattaccat attgccgtat tgccgggaga cggtattggt
     6841 ccggaagtga tgacccaggc gctgaaagtg ctgaatgccg tgcgcaaccg ctttgcgatg
     6901 cgcatcacca ccagccatta cgatgtaggc ggcgcagcca ttgataacca cgggcaacca
     6961 ctgccgcctg cgacggttga aggttgtgag caagccgatg ccgtgctgtt tggctcggta
     7021 ggcggcccga agtgggaaca tttaccacca gaccagcaac cagaacgcgg cgcgctgctg
     7081 ccgctgcgta agcacttcaa attattcagc aacctgcgcc cggcaaaact gtatcagggg
     7141 ctggaagcat tctgtccgct gcgtgcagac attgccgcta acggcttcga catccagtgc
     7201 gtgcgcgaac tgaccggcgg catctatttc ggtcagccaa aaggccgcga aggtagcgga
     7261 caatatgaaa aagcgtttga taccgaggtg tatcaccgtt ttgagatcga gcgtattgcc
     7321 cgcatcgcgt ttgaatctgc tcgtaaacgt cgccacaaag tcacctcaat cgacaaagct
     7381 aacgtgctgc aatcctctat tttatggcgt gaaatcgtca acgagatcgc cacggaatac
     7441 ccggatgtcg aactggcgca tatgtacatc gacaacgcca ccatgcagct gattaaagat
     7501 ccatcacagt ttgacgttct gctgtgctcc aacctgtttg gcgacattct gtctgacgag
     7561 tgcgcaatga tcactggctc gatgggcatg ttgccttccg ccagcctgaa cgagcaaggt
     7621 tttggactgt atgaaccggc gggcggctcg gcaccagata tcgcaggcaa aaacatcgcc
     7681 aacccgattg cacaaatcct gtcgctggca ctgctgctgc gttacagcct ggatgccgat
     7741 gatgcggctt ccgccattga acgcgccatt aaccgtgcat tagaagaagg cattcgcacc
     7801 ggggatttag cccgtggcgc tgccgccgtt agtaccgatg aaatgggcga tatcattgcc
     7861 cgctatgtgg cagaaggggt gtaatcatgg ctaaaacgtt atacgaaaaa ttattcgacg
     7921 ctcacgtagt gtacgaagcc gaaaacgaaa ccccgctgtt atatatcgac cgccacctgg
     7981 tgcatgaagt gacctcaccg caggcgttcg atggtctgcg cgcccacggt cgcccggtac
     8041 gtcagccggg caaaaccttc gccactatgg atcacaacgt ctctacccag accaaagaca
     8101 ttaatgcctg tggtgaaatg gcgcgcatcc agatgcagga gctgattaaa aactgcaaag
     8161 aatttggcgt cgagctgtat gacctgaatc acccgtatca ggggatcgtc cacgtaatgg
     8221 ggccggaaca gggcgtcacc ttgccgggga tgaccattgt ctgcggcgac tcgcataccg
     8281 ccacccacgg cgcgtttggc gcactggcct ttggtatcgg cacttccgaa gttgaacacg
     8341 tactggcaac acaaaccctg aaacagggcc gtgcgaagac catgaaaatt gaagtccagg
     8401 gcaaagccgc gccgggcatt accgcaaaag atatcgtgct ggcaattatc ggtaaaactg
     8461 gcagcgcagg cggcaccggg catgtggtgg agttttgcgg cgaagcaatt cgtgatttaa
     8521 gcatggaagg tcgtatgacc ctgtgcaata tggcgatcga aatgggcgca aaagccgctc
     8581 tggttgctcc tgacgaaacc acctttaact atgtcaaagg ccgtctgcat gcgccgaaag
     8641 gcaaagattt cgacgacgcc gttgcctact ggaaaaccct gcaaaccgac gaaggcgcaa
     8701 ctttcgatac cgttgtcact ctgcaagcag aagaaatttc accgcaggtc acctggggca
     8761 ccaatcccgg ccaggtgatt tctgtgaacg acaatattcc cgatccggct tcgtttgccg
     8821 atccggttga acgtgcgtcg gcagaaaaag cgctggccta tatggggctg aaaccgggta
     8881 ttccgctgac cgaagtggct atcgacaaag tgtttatcgg ttcctgtacc aactctcgca
     8941 ttgaagattt acgcgcggca gcggagatcg ccaaagggcg aaaagtcgcg ccaggcgtgc
     9001 aggcactggt ggttcccggc tctggcccgg taaaagccca ggcggaagcg gaaggtctgg
     9061 ataaaatatt tattgaagcc ggttttgaat ggcgcttgcc tggctgctca atgtgtctgg
     9121 cgatgaacaa cgaccgtctg aatccgggcg aacgttgtgc atccaccagc aaccgtaact
     9181 ttgaaggccg ccaggggcgc ggcgggcgca cgcatctggt cagcccggca atggctgccg
     9241 ctgctgctgt gaccggacat ttcgccgaca ttcgcaacat taaataagga gcacaccatg
     9301 gcagagaaat ttatcaaaca cacaggcctg gtggttccgc tggatgccgc caatgtcgat
     9361 accgatgcaa tcatcccgaa acagtttttg cagaaagtga cccgtacggg ttttggcgcg
     9421 catctgttta acgactggcg ttttctcgat gaaaaaggcc aacagccaaa cccggacttc
     9481 gtgctgaact tcccgcagta tcagggcgct tccattttgc tggcacgaga aaacttcggc
     9541 tgtggctcct cgcgtgagca cgcgccctgg gcattgaccg actacggttt taaagtggtg
     9601 attgcgccga gttttgctga catcttctac ggcaatagct ttaacaacca gctgctgccg
     9661 gtgaaattaa gcgatgcaga agtggacgaa ctgtttgcgc tggtgaaagc caatccgggg
     9721 atccatttcg acgtggatct ggaagcgcaa gaggtgaaag cgggagagaa aacctatcgc
     9781 tttaccatcg atgccttccg ccgccactgc atgatgaatg gtctggacag catcggcctg
     9841 accctgcagc acgacgacgc tattgcgtct tatgaagcaa aacaacctgc gtttatgaat
     9901 tagacccctt gcccggtcaa atgaccgggc tttccgctat cgtccacgtc atcaaacgtc
     9961 tttaaccttt gcggttaaaa ataatgcgac aacagaaata atcgcaatta cccagtagac
    10021 agcaaagtgc ccccaacttt gtgcaattgc tccctgaata acaccagcca gaattacccc
    10081 ggtagagata gtgttggtaa ataaggtggt agctgccccc gctcttccag gcattaaatc
    10141 ctgaaaccat agcatcccaa tacccgcaac aatgccgata aatacagcgt taaaaagttg
    10201 cagcgtcatc aacgccatac ggctatgaaa gaaaatcaat ccagtgtaaa acagcactcc
    10261 tgccaccact gcaatgacca tcattcgccg cttaccataa cgtttgacat agtagccagc
    10321 cagaatcatt gctggtattt ccagtccagc tgccgtcccc atcaggaaac ccgccagttt
    10381 gtctggcaat cctaaatcgc tactgatcca caatggcata tcaataatgt acatagtgtt
    10441 gcaggtccac attaacgtcg aggcgacaaa taacatccgt acgttactat cctgccagtc
    10501 accttgcatt gataaagcat tttccgacgg cagttctacc cgcgccacag acagaagcat
    10561 aaatgcaatc aataccagac tgagtgtgaa tatcccggcg gcaatcgaaa acatcactgt
    10621 aaagccgtaa ttcaacgcca gcataaaggc cagcggtgga ccgattaccc atgccagaga
    10681 aagctgcgca cgcatcaccg agctaaacat caccacttct cgcgccgagt tatccgcgta
    10741 ttcccgcgcc agagcaaata actgtggcat tgccgtattg gccagagatg ccagaagcac
    10801 accacaggtg ataagcgtca gataatgacg attaaatgca aacagtagcg cattgccaat
    10861 agccatcaaa cagcaaaata taatcagttt tcgccgatcg ccctgactgt cagaacgttt
    10921 tgccaaccag aggcttacgc cgatcccagc aatagcattc accgtataaa agagtccgat
    10981 ccagaaaggt tgcgcgccaa cctcacgact cagaaataag ctcaatgtag gagcctgtag
    11041 cgccccggcc acccccatca taaaagcgac cagcataaat gccgcgtaaa caccgttcat
    11101 acggcgagcc atcgtcatta tccagatcat acgttccctt tttagcgcgg cgagaataaa
    11161 aaaaaccagc aggtataatc tgctggcggg tgattttaca ccaatactca gtcacacatg
    11221 atgcaggcaa gtcaactttc agaattgcgg tcatcccact gcatcagtcc ttcaagcatg
    11281 gttaatcgtt gtgggacgct taaccaacgc aaccagcaca acttcgctgt cgcggtaaaa
    11341 tagtgctgat aaaactgacg catggggcac ctccttgctt catcgttagg aattattgac
    11401 ttaatatagg gaaaataaaa ttgctgtctt ttgcgcagga gttccccttt tatgccatct
    11461 gctcgtctgc aacaacagtt catccgcctg tggcaatgct gcgagggtaa atcgcaggac
    11521 acaacgctca acgaactggc agcgttattg agctgctcgc gtcgtcatat gcgtaccctg
    11581 ctcaacacca tgcaggatcg cggctggctg acgtgggaag cggaagtcgg gcgcggtaaa
    11641 cgctcgcgtc tgacattcct ctataccggg ctggcgcttc agcaacagcg ggcggaagac
    11701 ctgctggagc aggatcgtat cgaccaacta gtgcagttgg ttggcgacaa agcgactgtg
    11761 cggcaaatgc tggtttctca tctgggccgc agcttccgcc aggggcggca catcctgcgc
    11821 gtgctctact atcgtccgtt gcgtaatctg ctacctggca gcgcattgcg ccgttccgaa
    11881 acccatattg cccggcaaat cttcagttcg ctaacgcgca taaatgagga aaatggggaa
    11941 ctggaagcag acatcgccca ccactggcag caaatatcac cgcttcactg gcgtttcttt
    12001 ttgcgtccag gagtccattt tcatcatggt cgtgaactgg aaatggacga cgtgatcgcc
    12061 tctttaaaac gaatcaatac gctgccgctc tattcgcata ttacggacat tgtgtcgccg
    12121 acgccctgga cgctggatat ccatctcacg cagccggacc gctggttgcc gttactgctg
    12181 gggcaagttc cggcgatgat cctgccgtgc gaatgggaaa ccctcagtaa ctttgccagc
    12241 catcctattg gcaccggtcc gtatgcggtg attcgcaaca gcaccaatca actgaaaatt
    12301 caggcattcg atgacttctt cggttaccgg gcattaatcg acgaagttaa cgtctgggtt
    12361 ctgccggaaa ttgccgacga gccagccggt ggtctgatgc taaaaggtcc acagggcgag
    12421 aaaaaagaga ttgaaagccg cctggaggaa ggttgctact atttactgtt cgacagccgc
    12481 acccatcgcg gggcgaatca gcaagtcagg gactgggtaa gctatgtgct ttctccaact
    12541 aatctggtct atttcgctga ggaacagtac cagcaactgt ggttcccggc ttatggactg
    12601 ctcccccgtt ggcaccatgc ccgcaccata aagagcgaaa aaccggctgg cctggaaagc
    12661 ctcaccctga ccttttatca ggatcacagt gagcatcggg tgattgccgg gatcatgcag
    12721 cagattctgg caagtcacca ggtcacgctg gaaatcaaag agatcagcta cgatcagtgg
    12781 catgaaggag agatcgagag cgatatctgg cttaatagcg ccaactttac gctgccgctg
    12841 gatttttcgc tgttcgcgca cttgtgcgag gtgccgctgc tccaacactg tattccgatc
    12901 gactggcaag ccgacgccgc ccgctggcgc aatggcgaaa tgaacctggc gaactggtgc
    12961 cagcaactgg tcgccagtaa agcaatggtg ccgcttatcc accactggct gatcattcag
    13021 ggacaacgca gtatgcgcgg cctgcgtatg aataccctcg gctggttcga ttttaaatca
    13081 gcgtggtttg cgccaccgga tccatgattg ctggtagata acaaaatcac tacactaacg
    13141 ccgttctcaa cggggtgcca cgcaaacgcg tgcgctgaga aaatacccgt cgaacctgat
    13201 ccggataacg ccggcgaagg gatttgaggc tccttctcaa gtcctttgcc actctttttt
    13261 gaggtgcaaa gtgttaaaaa aatgtcttcc cctgctgttg ctgtgcacag cgcccgtttt
    13321 cgctaaaccc gttctgactg tttataccta cgattccttc gccgccgact gggggcctgg
    13381 tccaaagatc aaaaaagctt ttgaagccaa ctgtaactgc gaactgaaac tggtggcgct
    13441 ggaagatggc gtttcgcttc tcaaccgtct acggatggaa ggcaaaaata gtaaagccga
    13501 tgtggtgctg gggctggata acaatctgtt agacgccgcc agtaaaaccg gactatttgc
    13561 caaaagcggt gtggcagcgg atgccgttaa cgttcccggc ggctggaata atgacacttt
    13621 cgtcccgttt gattacggct atttcgcctt cgtctatgac aagaacaaac tgaaaaaccc
    13681 gccgcaaagc ctgaaagaat tggttgagag cgatcagaac tggcgggtga tttatcagga
    13741 tccgcgtacc agcacgccgg ggctgggtct gctgttgtgg atgcaaaaag tctatggcga
    13801 tgacgcccca caagcctggc aaaaactggc gaagaaaacc gtcacggtca ccaaaggctg
    13861 gagcgaagcc tacggcctgt ttttaaaagg tgaaagcgat ctggtactga gttacaccac
    13921 ctctccggct tatcacattc tcgaagagaa aaaagataac tacgccgccg cgaactttag
    13981 cgaaggtcac tatctgcagg tggaagtcgc cgcccgcacc gctgccagca agcagccgga
    14041 gctggcgcaa aaattcctcc agtttatggt ttctccggct tttcagaatg cgatcccaac
    14101 cggtaactgg atgtatccgg tggcaaatgt cacgctgcct gccgggtttg aacaattgac
    14161 caaaccggcc accacgctgg agttcacacc agctgaagtg gcggcacaac gtcaggcatg
    14221 gattagcgaa tggcaacgcg ccgtcagccg ttaattcccg gctggttaat tccaggtgta
    14281 agcgccgcca cgctggtggt agcggttgcg ctcgcggcgt ttctcgccct gtggtggaac
    14341 gcgccgcagg gtaactgggt ggcagtctgg caggacagct acctgtggca tgtggtgcgc
    14401 ttctccttct ggcaggcgtt tctctcggcg ctactctctg tcgtacccgc gatattcctc
    14461 gcccgcgccc tctatcgcag gcgctttccg ggtcggctgg cgctgttgcg tctgtgcgca
    14521 atgaccttga tcctcccggt gctagttgct gttttcggca ttcttagcgt ctatggtcgt
    14581 cagggctggc tggcatcgct ctgccaatcg ctcggtctgg agtggacctt ttcgccctac
    14641 ggcctgcaag gcattttgct ggcgcacgta ttttttaatc tgccgatggc gagccgctta
    14701 ttactccagg cactggaaaa cattcccggc gaacaacgtc agcttgccgc ccagcttggg
    14761 atgcgcggct ggcatttttt ccgcttcgtc gaatggccgt ggctacggcg acaaatcccg
    14821 ccggttgccg cgcttatctt tatgctctgt ttcgccagct tcgccaccgt gctatcgctg
    14881 ggcggcggtc cgcaggcaac cactatcgag ctggctattt atcaggcgct gagttacgac
    14941 tacgatcctg cccgcgcggc gatgctggcg ctgctccaga tggtgtgttg ccttgggctg
    15001 gtgctgttga gtcagcgact aagtaaggcc attgcgcccg gcaccacgct gctgcaaggc
    15061 tggcgcgatc cggacgatcg tctgcatagc cgcatttgcg acacggtgtt aatagtgctg
    15121 gcgctgctgc tgttgctgcc accgttgctg gcggtgatcg tcgatggggt aaatcgccag
    15181 ttgccggaag tgctggcaca accggtgctg tggcaggcgc tgtggacctc gttgcgtatt
    15241 gcgctggcgg caggtgtatt gtgcgtagtg ctgaccatga tgctgctatg gagcagtcgc
    15301 gaactgcggg cgcggcagaa aatgctggcg ggtcaggcgc tggagatgag cggcatgttg
    15361 atcctcgcca tgccggggat tgtgctggct accggcttct ttttactgct caacaacact
    15421 atcggcctgc cacaatctgc tgacggcatt gtgattttca ccaatgcgtt aatggcgatc
    15481 ccttatgcgc tgaaagtact ggaaaacccg atgcgcgata tcaccgcccg ctacagtatg
    15541 ttgtgtcagt cgctgggcat tgaaggctgg tcgcgcttaa aagtggtcga gctgcgcgcc
    15601 ctgaaacgtc cactggcgca ggcgctggct tttgcctgcg tgctgtcgat tggtgatttt
    15661 ggcgtagtgg cgttgttcgg taacgatgat ttccgtaccc tgccgtttta tctctaccag
    15721 caaattggct cctatcgcag ccaggacggc gcggtcaccg cgttaattct gctactactc
    15781 tgttttctgc tgtttaccgt gattgaaaaa ctaccggggc gaaatgttaa aactgactga
    15841 tatcacctgg ctttatcacc atttgccgat gcgttttagc ttaacggtag aacgcggcga
    15901 gcaggtggca atcctcgggc caagcggcgc gggtaaaagt accctgctga atttgattgc
    15961 cggttttctg acgccagcca gcggttcgct gactatcgat ggtgttgatc acaccacgac
    16021 gccgccgtca cgccgtccgg tgtcgatgct gtttcaggag aacaacctgt tcagccatct
    16081 gacggtcgcg cagaacatcg ggctggggct aaatccgggg ttgaaactga acgcggcaca
    16141 gcaggagaaa atgcacgcga ttgcccgtca gatggggatt gataatttaa tggcgcggtt
    16201 accgggcgag ctttccggcg gtcagcgaca gcgcgtggcg ttagcgcgtt gtctggtgcg
    16261 cgaacagccg attttattgc tcgatgaacc gttctctgcg ctcgatccgg cgttacgtca
    16321 ggagatgctg acgctggtga gcacgagctg ccagcagcaa aaaatgacgc tattgatggt
    16381 gtcgcacagt gtggaagatg cggcgcggat cgccacacgc tcggtggtgg tcgccgacgg
    16441 gcgcatcgcc tggcagggta agactgaaga attgttgagc ggtaaggcga gtgcttcggc
    16501 aatattgggg attacgggtt agtatgcact gtcagatgcg gcgtgaacac cttatccgac
    16561 ctacggacag tgcgttttgt aggcctgata agacgcggtc agcgtcgcat gaggcaaccc
    16621 gcacaagaca atcccctaaa ccccaaccac tttacgcaaa atatcgatat acaccggcat
    16681 caacgggtgg cgaattaaca ccaccagcgc caccacgccg atggcagaaa tcaacggcgt
    16741 cagccacaac aaacggccgc gcgacaaata atgacttaaa cgatcggttg ctttaccgct
    16801 gcgccataac cgccagcaca gccagccacc aacccacaaa aataccgccg tcgccagcag
    16861 caaccattta aactcgccgc tctgcattcc ggctggaata tcgatcgccg cgcccgccag
    16921 aatcccaggc aggaagtaaa acggtggcca cagcaggcag ccgataatat tcggcgtaat
    16981 gaattttgcc accggcagat ccagcattcc cgccaccatt ggcaccagcg gacgcgtcgg
    17041 gccaacaaag cgaccgacca gaatggtgaa catgctgtgt tgatgcaacg catgttcagt
    17101 tttatcgagt aatgctttgt ttttcttcag aaatgaccag cgatgcaacg gctttttaaa
    17161 acgccagccc agccagaagg agatccagtc gcccagcaga caaccgacaa tccctgccag
    17221 ccaagcgtgc cagaaactta actcgccgct gccaatcagc gctcccagcc ccgccatcag
    17281 caccgtaccg ggtagaatca aaccgaccag cgccagcgac tccagaaagg ccaccaacac
    17341 caccgccatc aatgaataca cggtagattg ggtaataaag tgttccagca atgcttgcat
    17401 aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc
    17461 cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt
    17521 cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg
    17581 agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg
    17641 tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc
    17701 taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct
    17761 gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag
    17821 cctcgcgtac acgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc
    17881 gcagtaacaa ttgctcaagc agattcatcg ccagcagctc cgaatagcgc ccttcccctt
    17941 gcccggcgtt aatgatttgc ccaaacaggt cgctgaaata cggctggtgc gcttcatccg
    18001 ggcgaaagaa acccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg
    18061 cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacaaccgt
    18121 agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcag acaaattctc
    18181 gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca
    18241 ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac
    18301 ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag
    18361 ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac
    18421 attgccgtca ctgcgtcttt tactggctct tctcgctaac ccaaccggta accccgctta
    18481 ttaaaagcat tctgtaacaa agcaggacca aagccatgac aaaaacgcgt aacaaaagtg
    18541 tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg
    18601 ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct
    18661 ctactgtttc tccatacccg tttttttgga tggagtgaaa cgatggcgat tgcaattggc
    18721 ctcgattttg gcagtgattc tgtgcgagct ttggcggtgg actgcgccag cggtgaagag
    18781 atcgccacca gcgtagagtg gtatccccgt tggcaaaaag ggcaattttg tgatgccccg
    18841 aataaccagt tccgtcatca tccgcgtgac tacattgagt caatggaagc ggcactgaaa
    18901 accgtgcttg cagagcttag cgtcgaacag cgcgcagctg tggtcgggat tggcgttgac
    18961 agtaccggct cgacgcccgc accgattgat gccgacggta acgtgctggc gctgcgcccg
    19021 gagtttgccg aaaacccgaa cgcgatgttc gtattgtgga aagaccacac tgcggttgaa
    19081 gaagcggaag agattacccg tttgtgccac gcgccgggca acgttgacta ctcccgctat
    19141 attggcggta tttattccag cgaatggttc tgggcaaaaa tcctgcatgt gactcgccag
    19201 gacagcgccg tggcgcaatc tgccgcatcg tggattgagc tgtgcgactg ggtgccagct
    19261 ctgctttccg gtaccacccg cccgcaggat attcgtcgcg gacgttgcag cgccgggcat
    19321 aaatctctgt ggcacgaaag ctggggcggc ttgccgccag ccagtttctt tgatgagctg
    19381 gacccgatcc tcaatcgcca tttgccttcc ccgctgttca ctgacacctg gactgccgat
    19441 attccggtgg gcaccttatg cccggaatgg gcgcagcgtc tcggcctgcc tgaaagcgtg
    19501 gtgatttccg gcggcgcgtt tgactgccat atgggcgcag ttggcgcagg cgcacagcct
    19561 aacgcactgg taaaagttat cggtacttcc acctgcgaca ttctgattgc cgacaaacag
    19621 agcgttggcg agcgggcagt taaaggtatt tgcggtcagg ttgatggcag cgtggtgcct
    19681 ggatttatcg gtctggaagc aggccaatcg gcgtttggtg atatctacgc ctggtttggt
    19741 cgcgtactcg gctggccgct ggaacagctt gccgcccagc atccggaact gaaagcgcaa
    19801 atcaacgcca gccagaaaca actgcttccg gcgctgaccg aagcatgggc caaaaatccg
    19861 tctctggatc acctgccggt ggtgctcgac tggtttaacg gtcgtcgcac gccaaacgct
    19921 aaccaacgcc tgaaaggggt gattaccgat cttaacctcg ctaccgacac tccgctgctg
    19981 ttcggcggtt tgattgctgc caccgccttt ggcgcacgcg caatcatgga gtgctttacc
    20041 gatcagggga tcgccgtcaa taacgtgatg gcgctgggcg gcatcgcgcg gaaaaaccag
    20101 gtcattatgc aggcctgctg cgacgtgctg aatcgcccgc tgcaaattgt tgcctctgac
    20161 cagtgctgtg cgctcggtgc ggcgattttt gctgccgtcg ccgcgaaagt gcacgcagac
    20221 atcccatcag cccagcaaaa aatggccagt gcggtagaga aaaccctgca accgcgcagc
    20281 gaacaggcac aacgctttga acagctttat cgccgctatc agcaatgggc gatgagcgcc
    20341 gaacaacact atcttccaac ttccgccccg gcacaggctg cccaggccgt tgcgactcta
    20401 taaggacacg ataatggcga tttttgataa ttatgaagtg tggtttgtca ttggcagcca
    20461 gcatctgtat ggcccggaaa ccctgcgtca ggtcacccaa catgccgagc acgttgttaa
    20521 tgcgctgaat acggaagcga aactgcccta caaactggtg ttgaaaccgc tgggcaccac
    20581 gccggatgaa atcaccgcta tttgccgcga cgcgaattac gacgatcgtt gcgctggtct
    20641 ggtggtgtgg ctgcacacct tctccccggc caaaatgtgg atcaacggcc tgaccatgct
    20701 caacaaaccg ttgctgcaat tccacaccca gttcaacgcg gcgctgccgt gggacagtat
    20761 cgatatggac tttatgaacc tgaaccagac tgcacatggc ggtcgcgagt tcggcttcat
    20821 tggcgcgcgt atgcgtcagc aacatgccgt cgttaccggt cactggcagg ataaacaagc
    20881 ccatgagcgt atcggctcct ggatgcgtca ggcggtctct aaacaggata cccgtcatct
    20941 gaaagtctgc cgatttggcg ataacatgcg tgaagtggcg gtcaccgatg gcgataaagt
    21001 tgccgcacag atcaagttcg gtttctccgt caatacctgg gcggttggct atctggtgca
    21061 ggtggtgaac tccatcagcg acggcgatgt taacgcgctg gtcgatgagt acgaaagctg
    21121 ctacaccatg acgcctgcca cacaaatcca cggcgaaaaa cgacagaacg tgctggaagc
    21181 ggcgcgtatt gagctgggga tgaagcgttt cctggaacaa ggtggcttcc acgcgttcac
    21241 caccaccttt gaagatttgc acggtctgaa acagcttcca ggtctgcccg tacagcgtct
    21301 gatgcagcag ggttacggct ttgcgggcga aggcgactgg aaaaccgccg ccctgcttcg
    21361 catcatgaag gtgatgtcaa ccggtctgca gggcggcacc tcctttatgg aggactacac
    21421 ctatcacttc gagaaaggta atgacctggt gctcggctcc catatgctgg aagtctgccc
    21481 gtcgattgcc gtagaagaga aaccgatcct cgacgttcag catctcggta ttggtggtaa
    21541 ggacgatcct gcccgactga tcttcaatac ccaaaccggt ccagcgattg tcgccagcct
    21601 gattgatctc ggcgatcgtt accgtctgct ggttaactgt atcgacacgg tgaaaacacc
    21661 gcactccctg ccgaaactgc cggtggcgaa tgcactgtgg aaagcgcaac cggatctgcc
    21721 aactgcttcc gaagcgtgga tcctcgctgg tggcgcgcac cataccgtct tcagccatgc
    21781 gctgaacctc aacgatatgc gccagttcgc cgagatgcac gacattgaaa tcacggtgat
    21841 tgataacgac acccgcctgc cagcgtttaa agacgcactg cgctggaacg aagtgtatta
    21901 cggatttcgt cgctaagtag ccacatcagg tatgtaacgc ctgatgcgac gctgacgcgt
    21961 cttatctggc ctacacgctg cgattttgta ggccggataa gcaaagcgca tccggcattc
    22021 aacgcctgat gcgacgctgg cgcgtcttat caggcctacg cgctgcgatt ttgtagaccg
    22081 gataagcgaa gcgcatccgg cacgaaggag tcaacatgtt aaaagatctc aaacgccagg
    22141 tattagaagc caacctggcg ctgccaaaac acaacctggt cacgctcaca tggggcaacg
    22201 tcagcgccgt tgatcgcgag cgcggcgtct ttgtgatcaa accttccggc gtcgattaca
    22261 gcgtcatgac cgctgacgat atggtcgtgg ttagcatcga aaccggtgaa gtggttgaag
    22321 gtacgaaaaa gccctcctcc gacacgccaa ctcaccggct gctctatcag gcattcccct
    22381 ccattggcgg cattgtgcat acgcactcgc gccacgccac catctgggcg caggcgggtc
    22441 agtcgattcc agcaaccggc accacccacg ccgactattt ctacggcacc attccctgca
    22501 cccgcaaaat gaccgacgca gaaatcaacg gcgaatatga gtgggaaacc ggtaacgtca
    22561 tcgtagaaac ctttgaaaaa cagggtatcg atgcagcgca aatgcccggc gttctggtcc
    22621 attcccacgg cccgtttgca tggggcaaaa atgccgaaga tgcggtgcat aacgccatcg
    22681 tgctggaaga ggtcgcttat atggggatat tctgccgtca gttagcgccg cagttaccgg
    22741 atatgcagca aacgctgctg gataaacact atctgcgtaa gcatggcgcg aaggcatatt
    22801 acgggcagta atgactgtat aaaaccacag ccaatcaaac gaaaccaggc tatactcaag
    22861 cctggttttt tgatggattt tcagcgtggc gcaggcaggt tttatcttaa cccgacactg
    22921 gcgggacacc ccgcaaggga cagaagtctc cttctggctg gcgacggaca acgggccgtt
    22981 gcaggttacg cttgcaccgc aagagtccgt ggcgtttatt cacgccgatc aggttccccg
    23041 cgctcagcat attttgcagg gtgaacaagg ctttcgtctg acaccgctgg cgttaaagga
    23101 ttttcaccgc cagccggtgt atggccttta ctgtcgcgcc catcgccaat tgatgaatta
    23161 cgaaaagcgt ctgcgtgaag gtggcgttac cgtctacgag gccgatgtgc gcccgccaga
    23221 acgctatctg atggagcggt ttatcacctc accggtgtgg gtcgagggtg atatgcacaa
    23281 tggcactatc gttaatgccc gtctgaaacc gcatcccgac tatcgtccgc cgctcaagtg
    23341 ggtttctata gatattgaaa ccacccgcca cggtgagctg tactgcatcg gcctggaagg
    23401 ctgcgggcag cgcatcgttt atatgctggg gccggagaat ggcgacgcct cctcgcttga
    23461 tttcgaactg gaatacgtcg ccagccgccc gctgctgctg gaaaaactca acgcctggtt
    23521 tgcaaaccac gatcccgatg tgatcatcgg ctggaacgtg gtgcagttcg atctgcgaat
    23581 gctgcaaaaa catgccgagc gttaccgtct tccgctgcgt cttgggcgcg ataatagcga
    23641 gctggagtgg cgcgagcacg gctttaaaaa cggcgtcttt tttgcccagg ctaaaggtcg
    23701 gctaattatc gacggtatcg aggcgctgaa atccgcgttc tggaatttct cttcattctc
    23761 gctggaaact gtcgctcagg agctattagg cgaaggaaaa tctatcgata acccgtggga
    23821 tcgaatggac gaaattgacc gccgtttcgc cgaagataaa cctgcgctgg caacttataa
    23881 cctgaaagat tgcgagctgg tgacgcagat cttccacaaa actgaaatca tgccattttt
    23941 actcgaacga gcaacggtga acggcctgcc ggtggatcgg cacggcggtt cggtggcagc
    24001 gtttggtcat ctctattttc cgcgaatgca tcgcgctggt tatgtcgcgc ctaatctcgg
    24061 cgaagtgccg ccgcacgcca gccctggcgg ctacgtgatg gattcacggc cagggcttta
    24121 tgattcagtg ctggtgctgg actataaaag cctgtacccg tcgatcatcc gcacctttct
    24181 gattgatccc gtcgggctgg ttgaaggcat ggcgcaacct gatccagagc acagtaccga
    24241 aggttttctc gatgcctggt tctcacggga gaaacattgc ctgccggaga ttgtgactaa
    24301 catctggcac gggcgcgata aagccaaacg tcagggtaat aagccgcttt cgcaggcgct
    24361 gaagatcatt atgaatgcct tttatggcgt gctcggcacc accgcctgcc gcttcttcga
    24421 tccgcgcctg gcatcgtcga tcaccatgcg tggtcatcag atcatgcggc agaccaaagc
    24481 attgattgaa gcgcagggct acgacattat ctacggagat accgactcga cttttgtctg
    24541 gctaaaaggt gcacattcgg aagaagaggc ggcgaaaatc ggtcgtgcac tggtgcagca
    24601 cgttaacgcc tggtgggcgg aaacgctgca aaaacaacgg ctgaccagcg cattagaact
    24661 ggagtatgaa actcatttct gccgttttct tatgccaacc attcgcggag ctgataccgg
    24721 cagtaaaaag cgctacgccg ggctgattca ggagggcgac aagcagcgga tggtgtttaa
    24781 agggctggaa accgtgcgca ccgactggac gccgctggcc cagcagtttc agcaggagct
    24841 gtacctgcgt atcttccgca acgagccata tcaggagtac gtccgccaaa ccatcgacaa
    24901 actgatggcg ggtgaactgg atgcgcggct ggtttaccgt aaacgccttc gccgtccgct
    24961 gagcgaatat cagcgtaatg ttccgcctca tgtacgcgcc gctcgccttg ccgatgaaga
    25021 aaatcaaaag cgcggtcgcc ccttgcaata tcagaatcgc ggcaccatta agtacgtatg
    25081 gaccaccaac ggcccggagc cgctggacta ccaacgttca ccactggatt acgaacacta
    25141 tctgacccgc cagctacaac ccgtggcgga gggaatactc ccttttattg aggataattt
    25201 tgctacactt atgaccgggc aacttgggct attttgagca aaaaaaagag ttcgccagat
    25261 accattttga tgcgtgacga atgctttgcc atccagtacc atagcgccct ttccattcct
    25321 ggacctgaat aacaccacta cctcataagc acggtagcgg gtggttattg cctgcaatta
    25381 aagatataga gccgaacaca tatgcctttt acacttggtc aacgctggat cagcgataca
    25441 gaaagcgaat tgggacttgg aaccgttgtc gcggtggatg cgcgaactgt cactttactt
    25501 ttcccatcta ctggtgaaaa ccgtctgtac gcacgcagtg attcccccgt gacccgcgtg
    25561 atgttcaacc ctggtgatac cattaccagc catgacggct ggcagatgca agtcgaagaa
    25621 gtaaaagaag aaaatggctt gctgacctat atcggtactc gcctggatac tgaagagtcc
    25681 ggcgtagccc tgcgtgaagt tttccttgat agcaaactgg tgttcagcaa accgcaggac
    25741 cgtctgtttg ccgggcagat tgaccgtatg gaccgctttg cgctgcgtta tcgcgcgcgt
    25801 aagtattcca gcgaacagtt ccgtatgccg tacagcggcc tgcgcggtca gcgtaccagc
    25861 ctgatcccgc atcagctcaa catcgctcat gatgttggtc gccgccacgc gccgcgcgtc
    25921 ctgctggctg acgaagtggg tttagggaaa accattgaag ccgggatgat cctgcatcag
    25981 caactgctct ctggcgctgc tgaacgtgtg ctgattatcg tcccggaaac cttacagcat
    26041 cagtggctgg tagaaatgct gcgccgtttc aacctgcgct ttgctctgtt tgatgatgag
    26101 cgttatgccg aagctcagca cgatgcttac aacccgtttg acaccgaaca gctggtgatt
    26161 tgctcgctgg attttgcccg tcgtagcaaa cagcgcttgg aacatctctg tgaagccgaa
    26221 tgggacctgc tggtggtcga tgaagcgcat cacctggtgt ggagcgaaga tgcgccaagc
    26281 cgtgaatatc aggccattga acaactggca gagcacgtgc cgggcgttct gctgctgacc
    26341 gcgaccccgg aacagctggg gatggaaagc cacttcgccc gtctgcgtct gctggacccg
    26401 aaccgtttcc acgattttgc gcagttcgtt gaagagcaga aaaattatcg tccggttgcg
    26461 gacgccgttg ccatgctgct ggcaggtaac aaactgagca atgacgaact gaacatgctc
    26521 ggcgagatga tcggcgagca ggatatcgag ccgctgttgc aggcagcaaa cagcgacagc
    26581 gaagatgccc agagcgcccg tcaggagctg gtttcgatgc tgatggatcg ccacggcacc
    26641 agccgcgtgc tgttccgtaa cacgcgtaac ggtgtgaaag gattcccgaa acgcgagctg
    26701 cacaccatta agctgccgct accgacgcag tatcagacgg ctattaaagt ctccggcatt
    26761 atgggcgcac gtaaaagtgc ggaagatcgt gctcgcgata tgctctaccc ggagcgtatt
    26821 tatcaggaat ttgaaggtga taacgccacc tggtggaact tcgatccgcg cgttgagtgg
    26881 ctgatgggct acctgaccag ccatcgctct cagaaagtgc tggtgatctg cgccaaagct
    26941 gccactgcgc tgcaactgga gcaggtactg cgcgaacgtg aaggtattcg cgctgcggtg
    27001 ttccacgaag gtatgtcgat tatcgaacgt gaccgcgctg ccgcctggtt tgccgaagaa
    27061 gacaccggcg cacaggtact gctgtgctca gaaatcggtt ctgaaggacg taactttcag
    27121 ttcgccagtc acatggtgat gtttgacctg ccattcaacc cggatctact ggagcagcgt
    27181 attggtcgtc tggatcgtat cggccaggcg cacgatattc agatccatgt gccttatctg
    27241 gagaaaaccg ctcagtcggt gctggtgcgc tggtatcacg aaggtctgga tgcatttgag
    27301 cacacctgcc cgaccggacg cactatttac gatagcgtat acaacgatct gattaactat
    27361 ctggcttcac cggatcaaac cgaaggcttt gacgatctga tcaaaagctg ccgcgagcaa
    27421 catgaagcgc tgaaagcaca gctggaacag ggtcgtgacc gcctgctgga aatccactcc
    27481 aacggtggcg aaaaagccca ggcactggca gaaagcattg aagagcagga tgacgatacc
    27541 aacctgatcg ccttcgccat gaacctgttc gatattatcg gtatcaatca ggacgatcgc
    27601 ggcgacaaca tgatcgtgct gacgccgtcc gatcatatgc tggtgccgga cttccctggc
    27661 ctgtcggaag atggcatcac catcaccttt gatcgtgaag tggcgctggc gcgtgaagat
    27721 gcacagttta ttacctggga gcatccgctg atccgcaacg gtctggatct gatcctttct
    27781 ggcgataccg gtagcagcac gatttcactg ttaaaaaaca aagcgttgcc ggtaggtacg
    27841 ctgttggtgg aactgattta tgtggttgaa gcccaggctc cgaagcagtt gcagctcaac
    27901 cgcttcctgc caccgacgcc ggtacgtatg ctgctggata aaaacggcaa caacctggcg
    27961 gcgcaggtag agtttgaaac ctttaaccgc cagcttaacg cggttaaccg tcacaccggc
    28021 agcaaactgg ttaacgccgt gcagcaggat gttcacgcta tccttcaact gggtgaagcg
    28081 cagatcgaga aatctgcccg tgcattgatt gatgcagcgc gtaacgaagc cgacgaaaaa
    28141 ctgtctgccg agctgtctcg tctggaagct ctgcgtgcag tgaacccgaa cattcgtgac
    28201 gacgaactga ccgccattga gagcaaccgt cagcaggtaa tggaaagcct ggatcaggca
    28261 ggttggcgtc tggatgccct gcgtttgatc gttgtaacgc atcagtaacg gagccgaaaa
    28321 tggggatgga aaactacaat ccaccgcagg aaccctggtt ggttatcctg tatcaggatg
    28381 accatattat ggtggtcaac aagccgagcg gtttgttgtc agtgccgggt cgtctggaag
    28441 agcacaaaga cagcgtgatg acgcgcattc agcgtgatta tccgcaggca gaatcggtgc
    28501 atcgtctgga tatggctacc agcggcgtga ttgtagtggc gctgaccaaa gccgcggagc
    28561 gggagttaaa acgccagttc cgcgagcgcg agccgaaaaa gcagtatgtg gcccgcgtct
    28621 gggggcatcc atcccccgca gaaggtctgg tggatctgcc gctgatttgc gactggccaa
    28681 accgcccgaa acagaaagtc tgttacgaaa cgggtaaacc tgcgcagacg gaatatgaag
    28741 tggtggagta tgcggcggat aacacggcaa gagtggtgtt aaaaccgatt accgggcgtt
    28801 cgcatcaatt gcgtgtgcat atgctggcgc tggggcatcc gattctcggc gatcgttttt
    28861 atgcatcacc agaagcgaga gcgatggcac cgcgtttgtt gctacatgca gagatgttga
    28921 cgattaccca tccggcgtat ggcaatagta tgacgtttaa agcgccagcg gatttttaat
    28981 cgtcacgctg gcgcagttcg gtaggcggat aaggcgttca cgccgcatcc ggcaattgtg
    29041 cgccgattgc ctgatgcgac gcgggccgcg tcttatcagg cctacagttt aaaacttatt
    29101 taaacccttt ctgctgcttt atcagctcat atgcctgctg aatttcctgc gctttctgct
    29161 tcgccatctc catcatctca ggcggcaaac ctttcgccac cagcttatcg ggatggtgtt
    29221 cactcatcag cttacggtag gcacgtttga tggtggtcgc atcatccgtc ggcttcacgc
    29281 ccagcacatt acaggcatct tccagcgtcg ggccacgctg cgcttgctgc cagttaccac
    29341 cgccagattg ctgctgataa ccgccgccaa actgtgcacc gccctgcatc atgcgcaaaa
    29401 actggtcaaa ctgggcgcgg gagatcccta attcttcagc aatgacatac agcaccgccc
    29461 gttcattcgg gtgcagtgaa ccatcagcaa acgccgcctg aatctggatc tccagaaaca
    29521 tacgaattaa gtcaaaacga ccaaagcaga cactgcgaaa ctggcgcatc ttttcgcgca
    29581 gcgggtaatt atctgatttt cccacccgga acgcgttttg cgccgcagta cgggaagcgc
    29641 catgaaggtt cattcggtcc atcaactggc tggcgatatg aatatcagcc tccgtgacgc
    29701 gacctttgga tttggttaaa tgccccatca cttcaaaagt ggtggcaaaa aacagcgcct
    29761 gacgctcacg ctggttggcg aaccacgcca ttttacggct acgggcttta tcaaacatat
    29821 ggccaattaa caggcctaac actacgcccc aaaagccgcc gcccatcagt aaggccacgg
    29881 ccacgccaat gatttttccc caatactgca tatattcccc aaatcgacac acggatatca
    29941 gggctatctc ccacaatata aaggtgcttt taccgttttc cggctgcggt caattgtggg
    30001 acatcgccta taatttgcat tatcatacct gtcattcaat gccgtgccta acaccactga
    30061 cgctattcgg acaggattaa cactagcgta gagatgacga gtacgttagt ctctgaccgt
    30121 ttgtcacgcg caacgttacc gatgatggaa caataaaatc aacgtatgaa aaaacgtatc
    30181 cccactctcc tggccaccat gattgccacc gccctttata gtcaacaggg actggcagcc
    30241 gacctcgcct cacagtgcat gttgggcgtg ccaagctatg accgtcctct ggtacagggc
    30301 gataccaatg acttacccgt gactatcaat gctgaccacg cgaaagggga ctacccggat
    30361 gacgccgtgt ttactggcag cgtggatatc atgcagggta acagccgtct gcaggccgac
    30421 gaagtgcagc tccatcaaaa agaggcacca ggacaaccgg agccggtacg taccgttgat
    30481 gcgctcggta atgtccatta cgacgataac caggtgatcc tcaaagggcc gaaaggctgg
    30541 gcgaatctga acaccaaaga taccaacgtc tgggaaggtg attaccagat ggtgggtcgc
    30601 cagggtcgcg gtaaagcgga cctgatgaaa caacgtggcg aaaaccgcta taccattctg
    30661 gataacggta gctttacctc ctgtctgccg ggttctgaca cctggagcgt ggtaggtagc
    30721 gaaattattc atgaccgcga agaacaagtt gctgagatct ggaacgcccg ctttaaggtg
    30781 ggtccggtac cgatctttta tagcccctat ttgcagttgc cggtgggtga caaacgtcgc
    30841 tctggtttct tgatcccgaa cgccaagtac accaccacca actactttga gttctacctg
    30901 ccatattact ggaacatcgc gccaaatatg gatgccacca tcacgccgca ttatatgcat
    30961 cgtcgtggca acatcatgtg ggagaacgaa ttccgctacc tctcccaggc gggcgctggc
    31021 ttgatggaac tggactatct gccttcagat aaagtctatg aagatgaaca cccgaacgat
    31081 gacagttcac gtcgttggtt attctactgg aaccactccg gggtcatgga tcaggtgtgg
    31141 cgtttcaacg tcgactacac caaggtcagc gatcctagct acttcaatga tttcgataac
    31201 aagtacggtt ccagtactga cggctacgca acgcaaaaat tcagcgttgg ctatgcggtg
    31261 caaaacttca atgccaccgt ttcaaccaag cagttccagg ttttcagcga acagaacacc
    31321 agtagctact cggcagagcc gcagttagac gttaattact accagaatga tgttggtccg
    31381 tttgatacgc gtatttacgg ccaggcagtg cactttgtta acaccagaga cgacatgcct
    31441 gaagcaaccc gtgttcacct ggaaccgacc atcaatttgc cgctctctaa taactggggc
    31501 agcatcaata ccgaagcgaa gtttctggca acccattatc agcaaaccaa tcttgactgg
    31561 tataactcca gaaacacgac caagctggac gaatccgtta accgcgtaat gccgcaattc
    31621 aaagttgacg gcaaaatggt ctttgaacgc gatatggaaa tgctggctcc gggttatacc
    31681 caaacgctgg aaccgcgcgc gcagtatttg tacgtgccgt atcgcgatca gagcgacatc
    31741 tataactacg actcgtctct gctgcaatct gactactctg gcctgttccg ggaccggact
    31801 tacggcggtc ttgaccgtat tgcctccgct aaccaagtga cgaccggtgt cacatctcgc
    31861 atatatgatg atgctgccgt tgaacgtttt aatatttccg ttggtcaaat ctactatttc
    31921 acggagtctc gcactggcga tgacaacata acatgggaga atgacgacaa aacgggctca
    31981 ctggtgtggg caggcgacac ctactggcgt atctccgagc gttggggatt gcgtggcggg
    32041 attcagtacg atacacgtct ggataacgta gcgaccagta actccagcat tgaataccgt
    32101 cgggatgaag accgtctggt acagctgaat taccattacg ccagcccgga atatattcag
    32161 gctacgctgc ctaagtacta ttccactgct gagcaatata agaatggtat ttcgcaggta
    32221 ggtgctgtcg ccagcaggcc aattgccgat cgttggtcca ttgttggggc ctactactac
    32281 gacaccaatg cgaacaagca agccgactct atgttaggtg tgcaatacag ctcctgctgc
    32341 tatgcaattc gcgtcggtta cgagcggaag ctgaacggtt gggataacga taaacaacat
    32401 gcggtatatg acaacgcaat cggctttaac atcgaacttc gcggcctgag ctccaactac
    32461 ggtctgggta cgcaagagat gctgcgttcg aacattctgc cgtatcaaaa cactttgtga
    32521 tttgttgatt taccacgtaa tccgcagtgc ggttaattga aatggaaaaa gtatgaagaa
    32581 ctggaaaacg ctgcttctcg gtatcgccat gatcgcgaat accagtttcg ctgcccccca
    32641 ggtagtcgat aaagtcgcag ccgtcgtcaa taacggcgtc gtgctggaaa gcgacgttga
    32701 tggattaatg cagtcggtaa aactgaacgc tgcgcaggca aggcagcagc ttcctgatga
    32761 cgcgacgctg cgccaccaaa tcatggaacg tttgatcatg gatcaaatca tcctgcagat
    32821 ggggcagaaa atgggagtga aaatctccga tgagcagctg gatcaggcga ttgctaacat
    32881 cgcgaaacag aacaacatga cgctggatca gatgcgcagc cgtctggctt acgatggtct
    32941 gaactacaac acttatcgta accagatccg caaagagatg attatctctg aagtgcgtaa
    33001 caacgaggtg cgtcgtcgca tcaccatcct gccacaggaa gtcgaatccc tggcgcagca
    33061 ggtgggtaac caaaacgacg ccagcactga gctgaacctg agccacatcc tgatcccgct
    33121 gccggaaaac ccgacctctg atcaggtgaa cgaagcggaa agccaggcgc gcgccattgt
    33181 cgatcaggcg cgtaacggcg ctgatttcgg taagctggcg attgctcatt ctgccgacca
    33241 gcaggcgctg aacggcggcc agatggggtg gggccgtatt caggagttgc cggggatctt
    33301 cgcccaggca ttaagcaccg cgaagaaagg cgacattgtt ggcccgattc gttccggcgt
    33361 tggcttccat attctgaaag ttaacgacct gcgcggcgaa agcaaaaata tctcggtgac
    33421 cgaagttcat gctcgccata ttctgctgaa accgtcgccg atcatgactg acgaacaggc
    33481 ccgcgtgaaa ctggagcaaa ttgctgctga tatcaagagt ggtaaaacga cttttgctgc
    33541 cgcagcgaaa gagttctcgc aggatccagg ttctgctaac cagggcggtg atctcggctg
    33601 ggctacacca gatattttcg atccagcctt ccgtgacgcc ctgacccgcc tgaacaaagg
    33661 tcaaatgagt gcaccggttc actcttcatt cggctggcat ttaatcgaac tgctggatac
    33721 ccgtaatgtc gataaaaccg acgctgcgca gaaagatcgt gcataccgca tgctgatgaa
    33781 ccgtaagttc tcggaagaag cagcaagctg gatgcaggaa caacgtgcca gcgcctacgt
    33841 taaaatcctg agcaactaat ggttaaaacc caacgtgttg tgatcactcc cggcgagccc
    33901 gccgggattg gcccggactt agttgtccag cttgcacagc gtgagtggcc ggtcgaactg
    33961 gttgtttgtg ccgatgccac tctccttacc gaccgggcag cgatgctcgg tttgccgctc
    34021 accctccgcc cttattcccc caactcccct gcacaaccgc aaactacggg cacattaacg
    34081 ctacttcctg tcgcgctacg tgaatctgtc actgcggggc agttagcgat tgaaaatggg
    34141 cattacgtgg tggagacgct ggcgcgagcg tgtgatggct gtctgaacgg tgaatttgct
    34201 gcgctgatca caggccccgt gcataaaggc gtcattaacg acgcaagcat tccgtttacc
    34261 ggtcataccg agtttttcga agagcgttcg caggcgaaaa aagtggtgat gatgctggcg
    34321 accgaagaac ttcgcgtggc gctggcaacg acgcatttac cgctgcgcga tatcgcagat
    34381 gctatcaccc ctgcactttt gcacgaagtg attgctattt tgcatcacga tttgcgtaca
    34441 aaatttggta ttgccgaacc gcgcattctg gtctgcgggc tgaatccgca cgcgggcgaa
    34501 ggcggtcata tgggtacgga agagatagac accattattc cggtgctcga cgagctgcgg
    34561 gcgcagggga tgaaactcaa cgggccgctg cctgccgata ccctgtttca gccgaaatat
    34621 ctcgataacg ccgacgccgt gctggcgatg taccacgatc agggtcttcc cgtgctaaaa
    34681 taccagggct tcgggcgcgg tgtgaacatt acgctgggcc tgccctttat tcgcacatca
    34741 gtggaccacg gcaccgcgct tgaactggcg ggacgtggcg aagccgatgt cggcagtttt
    34801 attacggcgc ttaatctcgc catcaaaatg attgttaaca cccaatgaat aatcgagtcc
    34861 accagggcca cttagcccgt aaacgcttcg ggcaaaactt tctcaacgat cagttcgtga
    34921 tcgacagcat tgtttctgcc attaacccgc aaaagggcca ggcaatggtc gaaatcggcc
    34981 ccggtctggc ggcattgacc gagccggtcg gcgaacgtct ggaccagctg acggtcatcg
    35041 aacttgaccg cgatctggcg gcacgtctgc aaacgcatcc attcttaggc ccgaaactga
    35101 cgatttatca gcaggatgcg atgaccttta actttggcga actggccgag aaaatgggtc
    35161 agccgctgcg tgttttcggc aacctgcctt ataacatctc cacgccgttg atgttccatc
    35221 tgtttagcta tactgatgcc attgccgaca tgcactttat gttgcaaaaa gaggtggtga
    35281 atcgtctggt tgcaggaccg aacagcaaag cgtatggtcg attaagcgtc atggcgcaat
    35341 actattgcaa cgtgatcccg gtactggaag ttccgccgtc agcctttaca ccaccaccca
    35401 aagtggattc cgccgtcgtg cgcctggtcc ctcatgcaac gatgcctcac ccggttaaag
    35461 atattcgcgt gttgagccgc atcaccaccg aagcctttaa ccagcgtcgt aaaaccattc
    35521 gtaacagcct cggcaacctg tttagcgtcg aggtgttaac cggaatgggg atcgacccgg
    35581 cgatgcgagc ggaaaatatc tctgtcgcgc aatattgcca gatggcgaac tatctggcgg
    35641 agaacgcgcc tttgcaggag agttaacgat gatcaattcg ccccgagtgt gtattcaggt
    35701 tcaaagcgtc tacattgagg cacaatcttc acctgataat gaacgttacg tttttgctta
    35761 taccgtaacc atacgcaatc tggggcgagc gccagtgcag ttgttggggc gttactggct
    35821 gatcaccaat ggcaatggcc gtgaaaccga agtccagggc gaaggagtgg ttggcgtcca
    35881 gccacttatc gcgcctggcg aagagtacca gtacaccagc ggtgcaatca ttgaaacccc
    35941 gctgggcacc atgcagggtc actacgaaat gatcgatgaa aatggcgtcc ctttcagcat
    36001 cgacattccc gtattccgac tcgccgttcc cacactcatt cattaaaaga atatggcgac
    36061 ataccttatt ggcgacgttc atggttgtta cgatgaactg atcgcattgc tgcataaagt
    36121 agaatttacc cccgggaaag ataccctctg gctgacgggc gatctggtcg cgcgcggccc
    36181 gggttcgctg gatgttctgc gctatgtgaa atccttaggc gacagcgtac gtctggtgct
    36241 gggcaatcac gatctgcatc tgctggcggt atttgccggg atcagccgca ataaaccgaa
    36301 agatcgcctg acaccgctgc tggaagcgcc ggatgccgac gagctgctta actggctgcg
    36361 tcgccagcct ctgctgcaaa tcgacgaaga gaaaaagctg gtgatggccc acgcagggat
    36421 cacgccgcag tgggatctgc agaccgccaa agagtgcgcg cgcgatgtag aagcggtgct
    36481 gtcgagtgac tcctatccct tctttcttga cgctatgtac ggcgatatgc caaataactg
    36541 gtcaccggaa ttgcgggggc tgggaagact gcgttttatc accaacgcct ttacccgtat
    36601 gcgtttttgc ttcccgaacg gtcaactgga tatgtacagc aaagaatcgc cggaagaagc
    36661 ccctgcccca ctgaaaccgt ggtttgcgat tcctggccct gtcgctgaag aatacagcat
    36721 cgcctttggt cactgggcat cgctggaagg caaaggtacg ccggaaggta tttacgcgct
    36781 ggataccggc tgctgctggg gtgggtcatt aacctgcctg cgctgggaag ataaacagta
    36841 ttttgtccag ccgtcgaacc ggcataagga tttgggcgaa gcggcggcgt cttaaacaca
    36901 gcctgataca ggaaggccgg ataagacgcg accggcgtcg catccggcac tagccgtaaa
    36961 ttctatacaa acttaccgcc gctccagaat ctcaaagcaa tagctgtgag agttctgcgc
    37021 atcggcgtcg tggaattcgc tgaataccga ttcccagtca tccggctcgt aatccgggaa
    37081 atgggtgtcg ccttccactt ctgcgtcgat atgcgtcaga tacagttttt gcgcttttgg
    37141 caggaactgc tcataaacgc gaccgccgcc aatcaccatg atttctggta cgtcaccaca
    37201 cgccgcaatg gcttcatcca ccgatttcac ccacgttacg cgatcgtccg tacccggttg
    37261 actgctgagg ataatatttt tgcgtcctgg caacggacga ccgattgatt cccaagtatg
    37321 gcggcccata atcacgggtt tatttaaggt gttgcgttta aaccaggcga gatcggcagg
    37381 caggttccac ggcatggcgt tttccatgcc gataacgcga tctaccgcta acgccgcaat
    37441 cagactgatc attgagattt cccgataaaa aaattgtcgc cactatacgt aaagcgtaaa
    37501 ccgtcgtcga ctggtgcgag gatgatgttg aggaaaattt tatattctgc tggcgagtcc
    37561 acgctctccc cctggactcg ccgcattaca atgaaacaaa aacaaacagt tagctgtaaa
    37621 gtgtgattta cgtcactctt tattaagatg agggtttcgt ttccggttca tccgccatgt
    37681 tgccggtatg tttaccttct tctgttccct gccagccatg tcgttgaatt aatgacagat
    37741 gttcgcggtc ctcggtgatg atctcactta acatcgcgct ggtgcgttta tagaccgccg
    37801 cgcgggcttt ggtgtcattc tcaaccattg ccatctcttc caccatctga atattaaagc
    37861 ggcggaacac atcggcacgt tctcgcgctt catacggccc cagacctaaa ctttccagcg
    37921 ccagacgccc ggttttcagc gcaccttcaa aggtttcacg ctccggcttt tcaacgcctg
    37981 cctgacgcaa acgaatgtag tgatcgacat cgcgggcgcg ggcaataatc tgcaaatgcg
    38041 ggaaatgttc tttcaccatc tccgtcagtt gcaggttggt ttgcggatcg tcaatagcgt
    38101 taatcagcac ttccgctttc gccgctccgg cagattccag taaatccatc cgcgtggcat
    38161 cgccataaaa cactttcata ccaaatttac gcagcgtttc gatatggtcc ggatcgtgat
    38221 cgagcaccac cattttcacc ccgctggaga gcagtaaacg tccggtaatc tgtccaaaac
    38281 gaccgaatcc ggcgataatc acgcgcggct gttcttcatc gatctcatcg gcttcacgcg
    38341 cctcctccgt agaagattgc tcaaggcgat tgaggatcac cagcagaatc ggcgttgctg
    38401 ccatcgacag cgccaccgcc agggtcagcg atttcaccca ctccggctcc agcacattcg
    38461 ccatctgcgc cgcaccaaat accacaaagg caaactcact gccctgccct aacaacaccg
    38521 caaaccagcg acgctgttta tttggcacct gcaacggtcg ggcaatcagc cacagcatgg
    38581 cgattttgat gatgaggaaa ccgagcagca aaatgacaat gcgcaatggg ttttcaatca
    38641 gcgtgccaaa gtctatcgac atgccaacac cgatgaaaaa cagccccaac agcaaacctt
    38701 taaacggttc gatatcgctt tccaacgcat gacggtattc cgagcttgcc agcagtacgc
    38761 ccgccagaaa cgcgcccatc gccatcgaca agccgacctc ttccagcagc aaaccaaagc
    38821 caaacacaag gaataacgcc acggcactaa acacttcccg caagccagag cgggcaacaa
    38881 aacgcagcgc cggacgcgta acatagcgcc ccagcaatac caccagtacc agcgcacccg
    38941 ccacttttaa cgccgagaga gcaaatgcgc ccatcgtcgt cgaggcactg ctcgccgcca
    39001 gtagcgggat catcgccacc agcgggattg ccgcgatatc ctggaacagc agcactgcaa
    39061 aggcactgcg gcccatttgc gtcaccatca gattgcgttc attcatcgcc tgcatggcaa
    39121 tcgccgttga tgagagcgcc agcgtcatac cgatcaattc cgcgacctgc cagcgcagcc
    39181 caagtaacat acagaacagc cccagcaggc caccgcaaat caccatctgc aatgcgccac
    39241 cgccgaacac tgccgcacgc agcttccaca gcctttgtgg atcgagttcg aggccgataa
    39301 taaacagcat cagcaccacc ccaatctcgg caaagtgcag aatagattcg gcatcggtca
    39361 ccagtcgcag cccccacggg ccaataatgc agccggcgat caggtagcca agtaccgatc
    39421 ccagaccaag acgtaccgca atgggtacaa tcagcgccgc cgatccgagg tagatcagcg
    39481 cctgaaccag cgtatgacta tccatgatgg gcctcctgcc attccagcag acgttgctta
    39541 tagtggcgcg cctgcccttc gagggtttcg tcgtcacaaa taaaggtgca gtgcatggca
    39601 aacggtggca gccagttcag cccgcagtag attgccgtcg cctgtagcgg ctgcgacagc
    39661 acatcaaagc ccggatgcgc accaatttca aaatggcttt ccccgccgcc ggtcgtcacc
    39721 gcccacagca aatgtttgcc atgcagcgcc gtgccgccgt gaccgtaagc ccagccgtgc
    39781 gacaaaactt tatcgatcca aagtttaaga agcggaggaa tgctgtacca ctgcatcggg
    39841 tgctgccaga cgatcagatc ggcgcgagag agcgcctcct gctcggcggc aatatcgata
    39901 ttgaagtcag gatagagttg ataaagagag cgaatttcga cgccttccag cgtccttgcc
    39961 tgttcaagca tccgtttatt cgcatgggaa tgatgcggat acggatgcgc ataaatgatg
    40021 aggatcatag ttattagccc gcgtgtttac gcctttgttt tcccacaaag tgtagtcata
    40081 tattcacgcg gcttatagtc aataatattg attcagaaaa gagagaaatc tgaacaaata
    40141 atcccattct ctgcccgcgg atcgtcatat tgctggtctg cgccagtgac attcctcgag
    40201 tctccggggc aaacgctacg gaaatcaaca agccaaacag cgagataccc gcccccatta
    40261 gcatcgtgtt actgataccg taattattga taaatatcgg cagtgcccag gtcgaaacaa
    40321 tggtgccaat acgacttaag gacataatca cgcccacggc agaggcgcgg atatccgtcg
    40381 ggaagagttc attaggatag agccactgca aattacccgg cccgccagag aaaaaggcat
    40441 acaccgcaaa ggccatcact accagccaga tccccatatc cgggattagc cccaaaaccg
    40501 ccaacgccag cgtcatcatg gcaaagctgc caatcaacaa tggacgccgt ccggcagtgt
    40561 ttagccacag catcggcgga atacagccga gcataaagaa caggctaatc accacattcc
    40621 ctagtgccgc gtttttgcca acccccaatc ccaacgaacc aacgatttgc gggccaaagg
    40681 tgtaaatggc gaacattggg atcacctggc aggtccagat ggcggcaaca aacagaacaa
    40741 aaggaaaatg gcggcgatta aacagatcgc gaaaacgggt ttgctgcggc tgctcttcat
    40801 cgaaagccac cggttcgcca aacagtttta tcatcatctc ttcgcactct tttactcgcc
    40861 ctttgcgtaa taaccagcgg ggagattctg gcagttcgaa tcgaccaatc aaaatcaaca
    40921 aacaagggat cgccgcgcta cccagcatcc agcgccaacc accttccaca tcataaagcc
    40981 agtagccgac cagatcggcg caggtcgcgc cgacatacca catcgcggca ataaagctga
    41041 tggaaaacgc ccgctgacgg gtactggaga actcggtgat catcgaagtg gcgatgggat
    41101 aatctgcacc gatgacaatg ccgatgagta cccgcatcac caacagttcg acgggggatg
    41161 aaacacacat cgtcgccacc gatatcacgc cgatggcgat gatatcaatg aggaacattt
    41221 tgcgccgtcc gactttatcg gagatatagc caaacagcga tgtgccaacg aacagcccgg
    41281 cgagcgttcc cgcgcccagc aagccaatcc agtcagcgtc cagtttcagc gccggagtca
    41341 gttgctccag cgccacgcca atcattacca gtacataacc atccagaaac ggaccaccgc
    41401 ttccccacag caaaatgcgg cggtgaatag aggagaattt gagatcgtca aagtttctgg
    41461 acggttgcat gggttattcc tgtttttttg caatgagagt cagtacggcg ttacgtatca
    41521 aaccaacatc agccgtaacg gaactccaca ccaaaggtgc cgcgcgggta ttcccactgt
    41581 tccagcgccg accccagccc cagaatgcga caggtgccgc actccagaca tccggcgtaa
    41641 tcgaagcgca cactgccgtc atcctgcttc ttgtacagac ctgcggggca cgctttcacc
    41701 agcagctcca gcacctgttt atcagcatcg gccttcacaa caatgtgcgg atgctcttca
    41761 tcgacattga atttattgac gcccagtttg acgtccacat tgacgggaga agtcataaaa
    41821 cggtcactcc tttcatgcca tccttgatca gattgatgaa gcccactttc ttgccgtggc
    41881 ggaggatttt cttgcgcatc agttccggcg cactgccgtc aatggtgaac aggtcacgcg
    41941 ccacgcccac cgccagttcc gggttgccgc taaacatgcg tgggttatca aggaacgccg
    42001 gtagtttctg gtacatgcgc atatcgcgca gcgggccact ctccagatgc tgacgatatt
    42061 ccgcgagttt ttgcttactg aaatcgtcgc ttttcatcgc tgaaagcacg gtttttgctg
    42121 cagcttcccc ggcggcaatc gccagatcca taccgcgaat ggtaaaaccg aggttcatac
    42181 acattccggc ggcatcaccg gcaatcaata cgccgtcacc gaccagctcc ggcagcatgt
    42241 tgatgcctgc ttccggcact acgtgagcgg aatattccac cagcttgccg cccgcgatca
    42301 gcggtgcaac ggccggatgc tgtttgaaat cttccagcat ttgcggcacc gattttttcg
    42361 cgtcatgcag atgatgcaga ccacaaacca gccccagcga cagggtattt tcattggtat
    42421 aaaggaagcc gccgcccatc aggccatcgg tgggtgatcc cgcaaacaga caagccgcgc
    42481 cctgattacc ctgcaactga aaacggtctt caattaccga cttcggtaac tcgatcagtt
    42541 ccttcacgcc aaccgccaca tccgtcggtt taacgcgttt tgccatcccc aacttttcgg
    42601 caaggatgga gttcacccca tcagcaagga gcaccgtttt cgcttcaatc acatcaccat
    42661 cggcttctac accgacgact ttgccatcgc gctgtacgag gttatcgacg cggatcccgg
    42721 taattaactg cgcgcccgct tcttcggcct gctccatcaa ccaggcatca aatttactgc
    42781 gcaaaacgga gtaagaacgc tgggatggcg aggtttcgtc accgttgcag tagtccatag
    42841 tcatcgctga cttttccgtc ataaacgcga gtttttcatg ggtgatcagg cgttctacgg
    42901 gggcggaatc ggcgaaacct ggaataatgt gttccagact gtgggcatag agacgcccgc
    42961 cagtaacgtt cttggcacct gcggaattgc cacgctcgat aactaacact tgcgcccctt
    43021 cgcgggcgag caccagtgcg gcaaccgaac cggcaagccc tgcaccgacg atgatggcgt
    43081 caaagatatc ttcggacata agagctccct gtcagcggca gagccgtaat gcagccctgc
    43141 cagagtggat caacgcgcta aagctgcggt cagtgccgga aggatcttca cggcatcgcc
    43201 aacaatgccg taatccgcgt actggaagat cggcgcattt ttatctttat tgatggcgaa
    43261 aatggtttgc gacgcgttag cgccaaccat gtgctggatc tacccggaga tccccaccgc
    43321 caggtacagt tcaggtttca gcatcaggtt ggagataccg acatagcgtt cgtgctccat
    43381 ccatttttcg ttttctgcca ccggacgaga acaggccaac tccgcaccta tcgccttgca
    43441 aagctgttct gccagcgcaa tgttctcttt gctgccaatg ccgcgaccga cgctgaccac
    43501 cagacgggct ttgtcgagat cgacgctgtt gctctggcgc gcctgggttg ccgtgcgggt
    43561 aatcgccaca gccggagcct gccactccac ggtgtgcgtt tcgccagtgc gtgacgcgtc
    43621 tggctgagcc gcatcgaacg tgccgctgct gatggtcagt accgcatacg gcgtggcaat
    43681 gcgttcttcg ccaatcgcca gaccgccgta aaccatgtgt ttcactgttg ctttaccgtc
    43741 ctgtacgctg acggtgctgg catcgttaga caccgccgct ttaaggcgat aacccagttt
    43801 tgccgccagt aatttgccgc gacgggtgtt tggcaacagc accagaccgt cagcgccgtg
    43861 ctggcgaata gtgtcagcca tgacaccggc gtaatcttcg atcatccgat cgtccggttt
    43921 gccgtttaat ttccagacat gattagcgcc gagctggatt gcctgtgcgc cgtcggcatc
    43981 attgaggaca aaggcattga tttgattagc taaagcctgc gcaccgttca tcagttccgg
    44041 cagacgagag ggggtatcgc tgaataccca gacttgagaa aacgtgttca tagcatcccc
    44101 tgtaattaaa tgactttgcg aagattttca gcaaatgcgg cgatctgttc ttcgccgtcg
    44161 ccttcaatca cgatgcgctg acgctcgcgc tgtttcggcg cggcaacctg ttgttcagac
    44221 caggcgtcca cggcgttaaa accaatatcc gccgccgacc atacctggac tggctttttc
    44281 gccgcgccga gaatggcttt catcgaagga atttgtgggg agttgatatc agtggaaaca
    44341 gcaacaaccg caggcagcgg aatgcttaag gtttcggttt catcttccag ttcgcgctca
    44401 acggtgaggg tatctgccgt cagggagata attttgctga cgccgttaac tgccggaata
    44461 ttgaggattt cgcccaccag cagaccaacc tgctgggcat aaaggtcgga agaaccatcg
    44521 ccacagagga tcagatcaaa gcctgctttc tgggcggctg cagccagtgc gctcgccgtt
    44581 tgttgcggca gtgcctgctc gaactggtca tcaatcacca caatcagttc atccgggccg
    44641 cgcgatagca catctttacg ccctttggcg ttggtcaggg ctttaccgcc cacacttaag
    44701 gctgtcacct gcgcctctgc tgcctgttgc tttagctggc aagccgcttc aatagcgttg
    44761 agatcgtatt ggcttatttt ggcatcggct ttgctgaagt ctaatgaacc atcagcatta
    44821 ttgaccgcaa tatcctgttc atcaggcacg cacttatagc aagtaataat cttcattgca
    44881 tctccagaaa tcatgaagga aacgtcgttc atatcggtgg tgttaattac agatatcacg
    44941 ccctttaatt tggaaataaa actttaatca ccaatattga aagtcagatg ctattttttt
    45001 acccacaaaa gaatataaca aactgattaa taaccaaaat taacgaaaaa cgccctctgt
    45061 tcactcgaaa caaaaatgtg ataccaatca cagaatacag cttattgaat acccattatg
    45121 agttagccat taacgcgtcc acgatgttaa taataattat attaaatgtt aacaaaaata
    45181 aaacaaacgg gaacgcaaaa taaatattcg ttttcacagt ggaattaact catgaagaat
    45241 gaaaagagaa aaacgggaat agaaccgaag gttttctttc cgccattaat aatcgtcggc
    45301 atactttgtt ggcttacagt cagagatctg gatgcggcga atgtcgttat taatgctgta
    45361 ttcagttacg tcaccaatgt atggggatgg gcatttgaat ggtatatggt ggtgatgctt
    45421 ttcggttggt tctggctggt gtttggcccg tatgccaaaa agcgtttagg taacgaaccg
    45481 ccagaattta gcaccgccag ttggatcttt atgatgttcg cctcctgtac gtctgctgcc
    45541 gtactgttct ggggatcgat tgagatctac tactacatct ccaccccgcc gtttggctta
    45601 gaaccgaact cgacaggagc gaaagagttg gggctggctt acagcttgtt ccactgggga
    45661 cctctgccgt gggccactta cagcttcctt tcagtcgcct tcgcttactt cttctttgtc
    45721 cgcaaaatgg aagtgattcg ccccagctcg acactggtgc cgctggcagg tgaaaaacac
    45781 gccaaagggt tgttcggtac catcgtcgac aacttctatc tcgtcgcctt gatcttcgcg
    45841 atgggtacca gtctgggcct tgccacgccg ctggtgaccg agtgtatgca atggttgttt
    45901 ggcattccgc ataccctgca actggacgct atcatcatta cctgctggat tatcctcaac
    45961 gccatttgcg tcgcttgcgg tctgcaaaaa ggggtacgta tcgccagtga cgtgcgtagt
    46021 tacctgagct tcctgatgct gggttgggtg ttcatcgtca gcggtgccag cttcatcatg
    46081 aactacttca ccgattcggt ggggatgttg ctgatgtatc tgccgcgcat gttgttctat
    46141 accgatccca tcgctaaagg cggcttcccg cagggctgga ccgtgttcta ctgggcatgg
    46201 tgggtgattt acgccatcca gatgagtatc ttcctcgccc gcatctcccg tggtcgtact
    46261 gtgcgtgaac tgtgcttcgg catggtgatg gggctgacag catccacctg gatcctgtgg
    46321 accgtgctcg gtagtaacac tctgctgttg atggataaaa acatcatcaa cattccaaat
    46381 ctgatcgaac agtacggtgt ggcgcgcgcc atcatcgaaa cctgggccgc tctgccactc
    46441 agcaccgcca ccatgtgggg cttcttcatc ctctgcttta ttgccaccgt tacgctggtt
    46501 aacgcctgct cttataccct ggcgatgtct acttgccgcg aagtacgcga tggtgaagaa
    46561 ccaccgctgc tggtgcgtat cggttggtca attctggttg gcattatcgg tattgttctg
    46621 ctggcgctcg gcggcctgaa accgattcaa accgccatta tcgccggagg atgcccgctg
    46681 ttcttcgtca acattatggt gacgctctcc tttattaaag acgcgaaaca gaactggaaa
    46741 gattaattaa cccccaaaat atcaagaggt tgaaagatgg attttaattt aaatgatgag
    46801 caggaactgt ttgtcgccgg tatccgcgaa ctgatggcca gcgaaaactg ggaggcctat
    46861 tttgccgagt gcgaccgtga cagcgtctac ccggaacgtt ttgtcaaagc actggcggat
    46921 atgggtatcg acagtctgct gatccctgaa gagcacggtg gtctggacgc ggggtttgtt
    46981 actctcgccg ccgtgtggat ggagctggga cgtctggggg caccaaccta tgtactgtac
    47041 cagctgccgg gcgggttcaa taccttcctg cgcgaaggca cacaagagca gatcgacaag
    47101 attatggctt tccgcggcac cggtaagcag atgtggaact cagcgattac cgaaccgggc
    47161 gcgggctccg acgtgggtag cctgaaaacg acttataccc gtagaaatgg taagatttat
    47221 cttaatggta gtaagtgttt tattaccagt agcgcctaca ccccgtacat cgtggtgatg
    47281 gcgcgcgacg gggcttctcc ggacaaacct gtctacaccg aatggtttgt tgatatgagc
    47341 aaaccgggca tcaaagtgac caaacttgag aagctcggtc tgcgtatgga tagctgctgt
    47401 gaaatcacct ttgacgatgt ggaactggac gagaaagaca tgttcggtcg ggaaggtaac
    47461 ggctttaacc gcgtcaaaga agagttcgac catgaacgtt tcctggtagc cctgaccaac
    47521 tacggtacgg cgatgtgcgc ctttgaagat gctgcgcgct acgccaacca gcgcgtgcag
    47581 tttggcgagg ctattggtcg tttccagttg attcaggaaa aattcgccca catggcgatc
    47641 aaattaaact ccatgaaaaa catgctgtat gaagcagcgt ggaaagcaga caacggcacc
    47701 atcacctctg gcgatgcagc gatgtgcaaa tacttctgcg ccaatgcggc atttgaagtg
    47761 gtggatagcg caatgcaggt gctgggcggt gtcgggattg cgggcaacca ccgcatcagc
    47821 cgcttctggc gtgacctgcg tgtagaccgc gtttccggag gatctgacga aatgcagatc
    47881 ctgacgctgg gtcgtgcggt gctgaagcaa taccgctaag tccgtgcgct gcctgatgcg
    47941 acgctgacgc gtcttatcag gcctacggga gcacgaatgt aagccggata aggcgtttac
    48001 gccgcatccg gcagtcatgc gctacataaa tttttcagga gctaataatg gatcatctac
    48061 ccatgccgaa attcggaccg ctggccggat tgcgcgttgt cttctccggt atcgaaatcg
    48121 ccggaccgtt tgccggacaa atgttcgcag aatggggcgc ggaagttatt tggatcgaga
    48181 acgtcgcctg ggccgacacc attcgcgttc aaccgaacta cccgcagctc tcccgccgca
    48241 atttgcacgc gctgtcgtta aatattttca aagatgaagg ccgcgaagcg tttctgaaat
    48301 taatggaaac caccgatatc ttcatcgaag ccagtaaagg tccggccttt gcccgtcgtg
    48361 gcattaccga tgaagtactg tggcagcaca acccgaaact ggttatcgct cacctgtccg
    48421 gttttggtca gtacggcacc gaggagtaca ccaatcttcc ggcctataac accatcgccc
    48481 aggccttcag tggttacctg attcagaacg gtgatgttga ccagccaatg cctgccttcc
    48541 cgtataccgc cgattacttt tctggcctga ccgccaccac ggcggcgctg gcagcactgc
    48601 ataaagtgcg tgaaaccggt aaaggcgaaa gtatcgacat cgccatgtat gaagtgatgc
    48661 tgcgtatggg ccagtacttc atgatggatt acttcaacgg cggcgaaatg tgcccgcgca
    48721 tgagcaaagg taaagatccc tactacgccg gttgcggtct gtataaatgt gccgacggct
    48781 acatcgtgat ggaactggtg ggcattaccc aaattgaaga gtgctttaaa gatattggcc
    48841 tcgcacatct actcggtacg ccggaaatcc cggaaggcac tcagcttatc caccgtatcg
    48901 aatgccctta cggcccactg gtagaagaaa aactcgatgc ctggctggcg gcacatacca
    48961 tcgcagaagt taaagaacgc tttgccgaac tgaatatcgc ctgtgccaaa gtactgaccg
    49021 taccggaact ggaaagcaat ccacagtatg ttgcccgtga atcaatcact cagtggcaaa
    49081 cgatggatgg tcgcacctgc aaagggccga acatcatgcc gaaattcaaa aataaccccg
    49141 gacaaatctg gcgcggaatg ccctcacatg gcatggacac ggctgccatt ttgaaaaata
    49201 tcggctacag cgaaaacgac attcaggagt tggtcagcaa aggtctggcc aaagttgagg
    49261 actaaacgcg tggcgtcagt cggcttgcct gactagcgcc cgcccaataa caaacgaaca
    49321 aaaatggata gaggtgcaat ggatatcatt ggcggacaac atctacgtca aatgtgggac
    49381 gatctggcgg acgtttacgg tcataaaacg gcgctgattt gtgaatccag cggcggagtc
    49441 gttaaccggt atagttatct tgagttaaat caggagatta accgcacggc aaacctgttt
    49501 tatacgctgg ggattcgcaa aggcaacaag gttgcactac atctcgacaa ctgcccggaa
    49561 tttatctttt gctggttcgg gctggcaaaa attggcgcga ttatggtgcc gattaacgcc
    49621 cgcctgttac gcgaggaaag cgagtggatc ttgcaaaata gccaggcgtg cctgctggtg
    49681 accagtgcgc aattttatcc tatgtatcaa cagattcagc aggaagatgc cactcaattg
    49741 cggcacattt gcctgacaga tgtggcactt cccgctgatg atggagtgag ttcatttact
    49801 caactgaaaa atcaacaacc tgccaccttg tgctatgcac cgccgctatc gactgacgat
    49861 acggcggaaa ttctcttcac ctccggcacc acctcccgac cgaaaggtgt ggtgattacc
    49921 cattacaacc tgcgcttcgc tggatattac tccgcctggc agtgtgcact gcgtgacgat
    49981 gacgtctaca tgacggtaat gcctgcgttt catatcgatt gccagtgtac tgcggcgatg
    50041 gcggcgtttt ctgccggggc cacctttgtg ctggtcgaga aatacagcgc ccgcgccttc
    50101 tggggacagg cgcagaagta ccgcgccacc attaccgaat gtattccgat gatgatccgt
    50161 acgttgatgg tgcagccgcc ttcagcgaac gatcggcaac accgcctgcg ggaagtgatg
    50221 ttttatctca acttgtcgga gcaggaaaaa gacgcatttt gtgaacgctt cggcgttcgc
    50281 ttgctgacgt cttatgggat gacggaaacc attgtgggca ttatcggcga tcgccctagc
    50341 gataaacgac gctggccgtc gattggtcgg gcggggtttt gctacgaagc ggagatccgc
    50401 gacgatcaca atcgcccgct cccggcaggt gagatcggtg aaatctgtat taaaggcgta
    50461 ccagggaaaa ccatcttcaa agagtatttt ctcaacccga aagccactgc gaaagtgctg
    50521 gaagccgacg gctggctaca taccggcgat accggatacc gcgacgaaga aggctttttt
    50581 tatttcgtcg atcgccgctg caatatgatc aaacgcggtg gcgagaatgt ctcctgcgtg
    50641 gagctggaaa atatcatcgc cacccatccg aaaattcagg acatcgtggt tgtgggtatt
    50701 aaagattcga ttcgcgatga agccatcaaa gcatttgtgg tgctgaatga aggtgaaaca
    50761 ttgagcgaag aggaattttt tcgcttctgc gaacaaaata tggcgaaatt taaagtgccc
    50821 tcttatctgg agatcagaaa agatctgcca cgtaattgct cggggaaaat aattagaaag
    50881 aatctgaaat aaatgaaacg tcagggaact accctgccag ctaataatca cgccctaaaa
    50941 caatatgcat tttttgcagg gatgttgtcg tccctaaaaa agcaaaaatg gagaaaagga
    51001 atgagtgaat cattacatct gacccgcaat ggatcaattc tggaaattac ccttgatcgc
    51061 ccaaaagcga atgctattga tgcaaaaacc agctttgaaa tgggcgaagt atttctaaat
    51121 ttccgtgacg atccgcaatt acgtgtcgcc attattaccg gtgccggaga gaaattcttt
    51181 tccgcgggct gggatttaaa agcggcagca gaaggcgaag caccggatgc tgactttggc
    51241 ccgggtggtt ttgcgggatt aaccgaaatt ttcaatctcg acaaaccagt tatcgcagct
    51301 gtgaacggct acgcctttgg cggcggtttc gaactggcgc tggcggcaga ttttattgtt
    51361 tgtgccgata acgccagctt cgccctgccg gaagccaaac tgggcatcgt tcctgacagc
    51421 ggcggtgtgc tgcgtctgcc gaagatcctg ccgcctacca tcgtcaatga aatggtgatg
    51481 accggcagac gaatgggcgc agaagaggcg ctgcgttggg ggatagtcaa ccgcgtggtt
    51541 agccaggcgg aactgatgga taacgcccgc gaactggctc agcagctggt taacagcgcc
    51601 ccgctggcga ttgcggcgct gaaagagatc taccgcacca ccagcgaaat gccggtagaa
    51661 gaagcgtatc gctatattcg cagcggcgtg ttgaaacact atccatcggt tctgcattcg
    51721 gaagatgcca ttgaagggcc gctggcgttt gccgagaagc gcgatccggt gtggaaagga
    51781 cgttaacaac cgtgagctat tacgcctttg aggggttaat tccggtggtt cacccgacgg
    51841 cgtttgtcca tcccagtgcc gtcttgattg gcgatgtgat tgtgggagcc ggtgtctaca
    51901 tcggcccact cgcctcactg cgtggtgact acgggcggtt gatcgtgcaa gcgggagcca
    51961 atattcagga tggctgcatt atgcatggct actgcgacac tgacaccatc gttggggaaa
    52021 acggccatat cgggcatgga gcgatcctgc atggttgtgt gattggtcgc gatgcattgg
    52081 tcgggatgaa cagcgtaatt atggatggcg cggtcattgg cgaagagagc attgttgccg
    52141 caatgagctt tgtcaaagca ggctttagtg gcgagaaacg ccagttgttg atgggtacgc
    52201 cagcccgcgc tgtacgcagt gttagtgacg acgagttaca ctggaaacgg ttgaatacca
    52261 aagagtatca ggatcttgtc gggcgctgtc atgcatcgtt acatgaaacg cagccactga
    52321 ggcaaatgga ggaaaatcgc ccccgtttgc aggggacgac ggatgtgacg cctaaacggt
    52381 gatcttgccg gatgcgacct ggttgctgga taagatgcta caacatcgta tctggcaagc
    52441 aggacgcccg gtatcaacga gataaaatta acgacgcata ctctttgaca acatcatctg
    52501 ccacttctgc tcgcggttaa gttccgtgag cggaacggca ggaatacgcg gtgtctttct
    52561 tacgccgact aatttttccc gactgttatt gcgcaggcgc gcgtaaattt gctcatcgat
    52621 atcgaccact ttaaccagtc gctgacactg gcatccccgc ccttccagct tattagggat
    52681 catcttaact tcgcagctta tttctgtgac ttccgacaga atataagtca gggtattaac
    52741 cgccttgctg tgttcaatat cgaaatgaat agagatctct cttgctatca cccaccgatt
    52801 ttcagccatc atccattcgg cgattaacaa gtagagtggt ttttcaacat atccttcaca
    52861 catacgaact ccagtttcac aaattaaaag tctagctatg actcgttttg aagcaattaa
    52921 acaaggccat attaaaattg tggatatatc gatagtatgc aacttcaccg tcgataagtg
    52981 tgagctaaat cctgcttatg tcatcaaaaa tatcgattcc ccaaaagatt tactta
//
LOCUS       DAJTQQ010000020        49664 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000020 DAJTQQ010000000
VERSION     DAJTQQ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49664
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.020"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..1058
                     /locus_tag="OO309_001275"
     CDS             <1..1058
                     /locus_tag="OO309_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5846478.1"
                     /translation="VEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATY
                     MIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGG
                     NIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIEL
                     VYVNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTD
                     KNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVP
                     ETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQA
                     EKYLLAQFGVDIGEAAQ"
     gene            1055..2020
                     /gene="yggF"
                     /locus_tag="OO309_001276"
     CDS             1055..2020
                     /gene="yggF"
                     /locus_tag="OO309_001276"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5846479.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2042..2551
                     /gene="fumE"
                     /locus_tag="OO309_001277"
     CDS             2042..2551
                     /gene="fumE"
                     /locus_tag="OO309_001277"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR5846480.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2548..3261
                     /locus_tag="OO309_001278"
     CDS             2548..3261
                     /locus_tag="OO309_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR5846481.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3233..3910)
                     /locus_tag="OO309_001279"
     CDS             complement(3233..3910)
                     /locus_tag="OO309_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5846482.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(3904..4581)
                     /locus_tag="OO309_001280"
     CDS             complement(3904..4581)
                     /locus_tag="OO309_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR5846483.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4569..4952)
                     /locus_tag="OO309_001281"
     CDS             complement(4569..4952)
                     /locus_tag="OO309_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR5846484.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(4968..5276)
                     /locus_tag="OO309_001282"
     CDS             complement(4968..5276)
                     /locus_tag="OO309_001282"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846485.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5277..5855)
                     /locus_tag="OO309_001283"
     CDS             complement(5277..5855)
                     /locus_tag="OO309_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR5846486.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(5878..6309)
                     /locus_tag="OO309_001284"
                     /pseudo
     CDS             complement(5878..6309)
                     /locus_tag="OO309_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6681..7700
                     /gene="epd"
                     /locus_tag="OO309_001285"
     CDS             6681..7700
                     /gene="epd"
                     /locus_tag="OO309_001285"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR5846487.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7750..8913
                     /gene="pgk"
                     /locus_tag="OO309_001286"
     CDS             7750..8913
                     /gene="pgk"
                     /locus_tag="OO309_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR5846488.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9128..10207
                     /gene="fbaA"
                     /locus_tag="OO309_001287"
     CDS             9128..10207
                     /gene="fbaA"
                     /locus_tag="OO309_001287"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR5846489.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10398..11258
                     /gene="mscS"
                     /locus_tag="OO309_001288"
     CDS             10398..11258
                     /gene="mscS"
                     /locus_tag="OO309_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR5846490.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11397..12032
                     /gene="argO"
                     /locus_tag="OO309_001289"
     CDS             11397..12032
                     /gene="argO"
                     /locus_tag="OO309_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR5846491.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12125..12865
                     /locus_tag="OO309_001290"
     CDS             12125..12865
                     /locus_tag="OO309_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR5846492.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13042..13992
                     /locus_tag="OO309_001291"
     CDS             13042..13992
                     /locus_tag="OO309_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5846493.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(13934..15412)
                     /gene="scpC"
                     /locus_tag="OO309_001292"
                     /pseudo
     CDS             complement(13934..15412)
                     /gene="scpC"
                     /locus_tag="OO309_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15436..16221)
                     /gene="scpB"
                     /locus_tag="OO309_001293"
     CDS             complement(15436..16221)
                     /gene="scpB"
                     /locus_tag="OO309_001293"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR5846494.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16232..17227)
                     /gene="meaB"
                     /locus_tag="OO309_001294"
     CDS             complement(16232..17227)
                     /gene="meaB"
                     /locus_tag="OO309_001294"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR5846495.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17220..19364)
                     /gene="scpA"
                     /locus_tag="OO309_001295"
     CDS             complement(17220..19364)
                     /gene="scpA"
                     /locus_tag="OO309_001295"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR5846496.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19568..20461)
                     /gene="argP"
                     /locus_tag="OO309_001296"
     CDS             complement(19568..20461)
                     /gene="argP"
                     /locus_tag="OO309_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR5846497.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            20844..21503
                     /gene="rpiA"
                     /locus_tag="OO309_001297"
     CDS             20844..21503
                     /gene="rpiA"
                     /locus_tag="OO309_001297"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR5846498.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21759..22991
                     /gene="serA"
                     /locus_tag="OO309_001298"
     CDS             21759..22991
                     /gene="serA"
                     /locus_tag="OO309_001298"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR5846499.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23220..23279
                     /gene="ibsC"
                     /locus_tag="OO309_001299"
     CDS             23220..23279
                     /gene="ibsC"
                     /locus_tag="OO309_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR5846500.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23612..23671
                     /locus_tag="OO309_001300"
     CDS             23612..23671
                     /locus_tag="OO309_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5846501.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23772..24374)
                     /locus_tag="OO309_001301"
     CDS             complement(23772..24374)
                     /locus_tag="OO309_001301"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR5846502.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24395..24578)
                     /gene="ssrS"
                     /locus_tag="OO309_001302"
     ncRNA           complement(24395..24578)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO309_001302"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24620..24949)
                     /gene="zapA"
                     /locus_tag="OO309_001303"
     CDS             complement(24620..24949)
                     /gene="zapA"
                     /locus_tag="OO309_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR5846503.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25117..25695
                     /gene="ygfB"
                     /locus_tag="OO309_001304"
     CDS             25117..25695
                     /gene="ygfB"
                     /locus_tag="OO309_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR5846504.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25721..27046
                     /gene="pepP"
                     /locus_tag="OO309_001305"
     CDS             25721..27046
                     /gene="pepP"
                     /locus_tag="OO309_001305"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR5846505.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27043..28221
                     /gene="ubiH"
                     /locus_tag="OO309_001306"
                     /gene_synonym="visB"
     CDS             27043..28221
                     /gene="ubiH"
                     /locus_tag="OO309_001306"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR5846506.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28244..29446
                     /gene="ubiI"
                     /locus_tag="OO309_001307"
                     /gene_synonym="visC"
     CDS             28244..29446
                     /gene="ubiI"
                     /locus_tag="OO309_001307"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR5846507.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29520..29603)
                     /locus_tag="OO309_001308"
     CDS             complement(29520..29603)
                     /locus_tag="OO309_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846508.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            29894..30988
                     /gene="gcvT"
                     /locus_tag="OO309_001309"
     CDS             29894..30988
                     /gene="gcvT"
                     /locus_tag="OO309_001309"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR5846509.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31012..31401
                     /gene="gcvH"
                     /locus_tag="OO309_001310"
     CDS             31012..31401
                     /gene="gcvH"
                     /locus_tag="OO309_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR5846510.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31520..34393
                     /gene="gcvP"
                     /locus_tag="OO309_001311"
     CDS             31520..34393
                     /gene="gcvP"
                     /locus_tag="OO309_001311"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR5846511.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34660..35403
                     /locus_tag="OO309_001312"
     CDS             34660..35403
                     /locus_tag="OO309_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5846512.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35460..36893)
                     /gene="bglA"
                     /locus_tag="OO309_001313"
     CDS             complement(35460..36893)
                     /gene="bglA"
                     /locus_tag="OO309_001313"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR5846513.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            36938..37248
                     /locus_tag="OO309_001314"
                     /pseudo
     CDS             36938..37248
                     /locus_tag="OO309_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37412..38071
                     /locus_tag="OO309_001315"
     CDS             37412..38071
                     /locus_tag="OO309_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR5846514.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38267..39247)
                     /gene="ygfZ"
                     /locus_tag="OO309_001316"
     CDS             complement(38267..39247)
                     /gene="ygfZ"
                     /locus_tag="OO309_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR5846515.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39490..39756
                     /gene="sdhE"
                     /locus_tag="OO309_001317"
     CDS             39490..39756
                     /gene="sdhE"
                     /locus_tag="OO309_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR5846516.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39737..40144
                     /locus_tag="OO309_001318"
     CDS             39737..40144
                     /locus_tag="OO309_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR5846517.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40184..40705)
                     /gene="fldB"
                     /locus_tag="OO309_001319"
     CDS             complement(40184..40705)
                     /gene="fldB"
                     /locus_tag="OO309_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR5846518.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40817..41713
                     /gene="xerD"
                     /locus_tag="OO309_001320"
     CDS             40817..41713
                     /gene="xerD"
                     /locus_tag="OO309_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR5846519.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41738..42448
                     /gene="dsbC"
                     /locus_tag="OO309_001321"
     CDS             41738..42448
                     /gene="dsbC"
                     /locus_tag="OO309_001321"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR5846520.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42454..44187
                     /gene="recJ"
                     /locus_tag="OO309_001322"
     CDS             42454..44187
                     /gene="recJ"
                     /locus_tag="OO309_001322"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR5846521.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44278..45376
                     /gene="prfB"
                     /locus_tag="OO309_001323"
     CDS             join(44278..44352,44354..45376)
                     /gene="prfB"
                     /locus_tag="OO309_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR5846522.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45386..46903
                     /gene="lysS"
                     /locus_tag="OO309_001324"
     CDS             45386..46903
                     /gene="lysS"
                     /locus_tag="OO309_001324"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR5846523.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(46946..47494)
                     /gene="idi"
                     /locus_tag="OO309_001325"
     CDS             complement(46946..47494)
                     /gene="idi"
                     /locus_tag="OO309_001325"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR5846524.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47549..47620
                     /locus_tag="OO309_001326"
     CDS             47549..47620
                     /locus_tag="OO309_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846525.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47617..47742)
                     /locus_tag="OO309_001327"
     CDS             complement(47617..47742)
                     /locus_tag="OO309_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846526.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47744..49192)
                     /gene="uacT"
                     /locus_tag="OO309_001328"
     CDS             complement(47744..49192)
                     /gene="uacT"
                     /locus_tag="OO309_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR5846527.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        12005 a        12416 c        13362 g        11881 t
ORIGIN      
        1 ttgtcgaagt gggtaaaaat ctcactggca aatataaaaa aggccagcgt tttgtattgc
       61 aaccggcgat ggggttacca agcggatatt cagcgggcta cagctacgaa tattttggcg
      121 gcaatgccac ttatatgatt attcccgaaa tcgccattaa tttgggctgc gtattaccgt
      181 atcacggctc ttattttgct gcggcgtcgc tggcagagcc tatgtgctgc attattggtg
      241 cttatcatgc caattatcac accacgcaat atgtttatga gcatcgcatg ggcgtcaaac
      301 ctggcggcaa tattgcactg ctggcgtgtg cgggtccgat gggcattggc gctatcgatt
      361 acgccattaa cggtggcata caaccgtcac gggtggtggt ggtcgatatc gacgacaaac
      421 gcctggcgca ggtgcagaag ctgctgccgg tggatctggc ggccagtaaa ggcattgagc
      481 tggtgtatgt gaataccaaa gggatgagcg atcctatcca gatgctgcgg gcgctgacag
      541 gagatgtcgg gttcgatgac atttttgttt atgcggcggt gcctgctgtc gttgagatgg
      601 ctgacgaatt actggcggaa gatggctgtc tgaacttctt tgccgggctg acggataaaa
      661 acttcaaagt gccgtttaat ttctacaacg tccattacaa cagcacgcac gtagtcggta
      721 catccggcgg ttcaacggac gacatgaaag aggcgattgc ccttagcgcc acaggacagt
      781 tacagccgtc gtttatggtg acccatattg gtggcctgga tgcggtgcca gaaaccgtgc
      841 tcaatctgcc ggatatccct ggcggtaaaa aactcattta taacggcgtg accatgccgc
      901 tcactgccat tgccgatttt gccgaaaaag gcaaaaccga tccgctgttt aaagagttgg
      961 cgcggctggt tgaggaaacg cacggcatct ggaatgaaca ggccgagaaa tatctgctgg
     1021 cacaatttgg cgttgatatc ggggaggccg cgcaatgatg tccctggcgt ggccattatt
     1081 tcgcgttacg gaacaggcag cgttggctgc ctggccgcaa accggatgtg gcgacaaaaa
     1141 taaaattgat ggcctggcgg tcaccgcgat gcgccaggca ttaaacgacg tcgcttttcg
     1201 tggacgagtg gttatcggcg aaggagaaat tgaccatgcg ccgatgctgt ggattggcga
     1261 agaggtgggc aaaggcgatg ggccagaagt cgatatcgcg gttgatccca ttgaaggcac
     1321 gcggatggtg gcgatggggc agagcaatgc gctggcggtc atggctttcg ctcctcgaga
     1381 tagcctgtta catgcgcccg atatgtatat gaaaaagctg gttgttaatc gactggctgc
     1441 cggggcgatt gatctgtctt tgcctctggc ggacaacttg cgtaacgtgg cgcgggcgtt
     1501 aggtaaaccg ctggacaagc tgcgcatggt tacgctggat aaaccgcgtc tgagtgccgc
     1561 gattgaagaa gcaacacagc tgggcgtgaa ggtttttgcc ctgccggatg gcgatgtcgc
     1621 cgccagcgtg ctgacctgct ggcaggataa tccttacgat gtgatgtaca ccatcggcgg
     1681 tgcgccagaa ggcgtgattt ctgcctgtgc cgttaaagcg ttaggcgggg atatgcaggc
     1741 cgagctgatt gacttttgtc aggctaaagg ggattacacg gaaaatcggc agattgcaga
     1801 gcaggagcgc aagcgttgta aggcaatggg cgttgacgtt aaccgtgtgt actcgctcga
     1861 tgaactggtg agggggaacg atatcctctt tagcgctacg ggcgtgacgg gcggcgagtt
     1921 ggtgaacggt atccaacaga cggcgaatgg ggtgcggacg cagacattac tgatcggcgg
     1981 cgcggaccaa acgtgtaata taatagactc cctgcattga tggtgatcta tcggaagacc
     2041 aatggcgacg ctgacagaag atgatgtgct tgagcaactg gatgcacagg acaatttatt
     2101 ttcatttatg aaaactgcgc attctatttt gctccagggg atacgccagt ttctgccgtc
     2161 gctgtttgtc gataacgatg aagagatcgt cgaatatgca gtgaagccgt tactcgccca
     2221 aagcggcccg cttgacgata ttgatgttgc gctgcgtttg atttatgcgc tggggaaaat
     2281 ggataaatgg ctgtatgccg atatcacgca cttttcccag tactggcatt acctgaacga
     2341 acaggatgaa acgcccggat ttgccgatga tataacctgg gattttatca gcaatgtcaa
     2401 cagtataact cgcaatgcga cgctctacga tgcgttaaaa gcgatgaagt tcgccgattt
     2461 cgccgtctgg tcagaggcgc gttttagcgg aatggtcaaa acggcgctga cgctggcagt
     2521 aacgaccacc ttaaaggaat taacgccgtg aaaattgaat taacggtgaa tgggctgaac
     2581 gtgcaagcgc agtaccatga tgaagagata gaacgcgtgc ataaactgtt gctgcgtatg
     2641 ctggcggcct tacagacggt aaatccgcag cggcgtacgg tggtttttct ttgcgctccg
     2701 ccggggacgg gtaaatctac cctaaccacc ttctgggaat atctcgcgca acaagacccg
     2761 gagctgcccg ctattcaggc gctcccgatg gatggttttc accattacaa tagctggctg
     2821 gacgcgcatc aattgcgccc cttcaaaggc gcaccagaga catttgacgt tgcgaaactg
     2881 gcggaaaatc tgcgccaggt ggtggaaggg gattgtacgt ggccgcagta cgatcgacaa
     2941 aagcatgatc ctgttgaaga tgcgttgcac gttaccgcac cactcgtcat cgtcgaagga
     3001 aactggttgt tgctggacga cgagaagtgg tgccagcttg cgcaattttg tgatttctcg
     3061 atctttatca acgcgccagc tgctgcgttg cgggagcgtc tggtggggcg caagctggcg
     3121 ggaggggtat cgctggcaga tgcagaagcc ttttatgacc gtaccgacgg gccgaatgtt
     3181 cgccgggggc tggaagagag tctgcctgca aatttgacct taatgatgac cgctacgggt
     3241 gaatatcgtc tggtggatta acgttcctga ttacaccatc ttccagccgc accacacgag
     3301 agaaatgacg ccgggtaaat gcggcgtcat gactgattgc taccacgctc gtgcctcgct
     3361 ggcggcactt ctccagccag ctttcaaaca cgctcaacca attttcgtca aaatcccgac
     3421 tcggctcgtc cagcagtaat agtggcggag agagtgcttc caggcaggcg acggcgacca
     3481 tccgtcgctg cgcattatgc agatccaacg ggtgcgagtt ggcagtgtct gctaactgac
     3541 aacattgcag ggcagcatgg gtacgttgag tgatttcatc cgccgggcat ttttgcagtt
     3601 tcaaaccaaa tgcgatctcg tcagcgacgg tgctgtgaaa aagttggttt tccgcttcct
     3661 gaaacagtac gccaaccttt gccgcgcgct ggcggttttt caggtttttc atcgcctgtt
     3721 gttgcagcat aaccgtaccg gctgtgggcg taagaagacc tgccattact cgtaacagcg
     3781 tcgatttccc tgcgccattg tcaccggtca gcgccaacca ttcaccttgt ttgagttgca
     3841 gagaaatgtc gcaaagacaa tccgttgccg cgccaggcca gcgataagat atcttattta
     3901 acgttaacat agtggctgga gcgctccgtt ttgcaattgc cacgcttgct ggcagtagtt
     3961 caggaaaggt gtgcgatgac gttcaaaaag aataatcagc gaaccacgtt caaacgccca
     4021 gtgttgtaag cgctgcaaca gcatttcgct ggcctgcggt gtcaggcggc taaaggcttc
     4081 atcaagaatt aagagctttg gctgcatcgc gatggcgcag gcgatgatca cgcgctgtgt
     4141 ctctccaccg gagagtgttg ccgggtgacg atggcgcaac ggctggcact cggtcagagt
     4201 cagggcggca tcaatccgcg ccataatctc tgcttcagca agacacagat tttctggccc
     4261 aaaggcaact tcttcttcca cgctaaaggt acagccggag agctgcaaat agggcgattg
     4321 ctggacgagc tggatggtgg cggattgttc attaagcggg agatgcccaa tgggcgtacc
     4381 gaggagcgtc cccgtgcctg ttatctctct tggcagaaag tccggatacc agcccgccat
     4441 cagctgtgcc agcgtacttt tgccactgcc gttgtcacca aagatggcga ccatccccgg
     4501 tttgacatag tgaaagtcat aacagaacgg aggatgcgtt gagtgtgtcg ggcaataacg
     4561 gaactgttct aacgtaacca tatccacgct ccgaattctg tcagcatcag caagatcatg
     4621 gtgtaacgcg ctacgcgttg tagggtgcta tcggctggtg cccacaacgt tgttcgtcgg
     4681 ttgtggatcc gaaatgcccg catatccagc gccgcaccac ggacagccag atcgttaagc
     4741 gcattgtgtg ttagcggaat aattaacgcg ggcatagcac gtaaacgctg ataccagccc
     4801 tcatccagcg gcacgccgcg ggcacgctgc gcttcatgaa taatcgctaa ttgccgtttt
     4861 aactgctcaa cgaccaataa cggcccggca aacagataag cgacgcctgg cggcaggcga
     4921 gaagcaaaca gagcgcgaat aaaacattgc acggggacgt actgcattca caactgtgag
     4981 gttgaaacaa tcgccagaat cagcaaccag agcgtaatag catgcgacca ccgttctggc
     5041 gagcgtggcg tgccgcttag ccattctggt agccagccac cgtgtaccag ccacagcccg
     5101 gctcccagcg agaacatcag ccagacgaca tattttgccc gccgccgcgt ggcctttagc
     5161 gcaataaggc agaaaaaggt cgccgcgcta tagatcggca aaatggtctg cgcgggcagg
     5221 ataagcgtgg tgcaggccgc taatgcccac agtgttaatg aggtaaatgg atgcatttag
     5281 cgcacggcag ccattgccgg aaaatggcgc gtggtgcgaa tcggcagctg gcgcagtaaa
     5341 agccagacaa tcaccgctgt aagaatttta tcgaccaggt tcgcgcctat caccgtaatc
     5401 gccaccgact ccaccaggtt ttgccccatc gagtgcatcc aggcaacgaa caaatcggct
     5461 ccgctaccgg tgacaccgcc gaacagcgca gtacgcagcg gtacagcgac gaccgttacc
     5521 gcaagcgtaa taatcacgcc gctgacaacg acttttggca gggtgcgaaa ccaacccgct
     5581 cgcgccagcc aaccggagac caggccgatg accatcgcga cgggtgcaaa tgcggcggcg
     5641 atcgggtcgg tcagcaatcc ccagagtaaa ttggtcagta agccggtcag cattccgatc
     5701 accggaccaa gcagtaccgc gctaataagt gtgccgatgg agtcgagaaa tataggtaat
     5761 ttgaccatgc tggccagttg cccgccgatc atattgatgg cgatagatat aacgatcaac
     5821 accagtgcct gactggaaaa gtggctacgc gccataagaa aacctctttg aaagtagtta
     5881 tttaacgggg gaaatatgca actcgtcgta accagaaagc gcgcaaggtt ggcggctaaa
     5941 cgccagttcc tcaaggctac cgatcaccag ttcaagcgtg gtccgcacgg tacagccggg
     6001 catcatatga ccgccaagca tcgtgccgtg tggatcggaa acgcagagat gaagatgctc
     6061 accactctgt tccagcgtgc cgtttagtga gatcacttcg aatgtaccgt tcaggagcgt
     6121 ggtgccttct tgcccggcat agcgcaaggc gacgtcagtc aggctaccgg tacagcctgc
     6181 aatctaggcg gcatgaagtt gatgttgctg tgcaaaagcg tgtagctggg aaaaaacttc
     6241 ctgcccgggg aggaggcgca gtgcgtaaaa gcgtgcggag gaagaatatg agtagggcaa
     6301 tgtcatcatt ggagtactcc gaaactcgga cgtattgcgg ataatatcga cgctgcatca
     6361 gtcatattct gatataagtc agggtttgtg acggcacaac gaaaagattt tgctacgctt
     6421 attcgtgatt ctgcaaagtg gaataaattc cacaggggcg gggctgaaaa cgtgacgtaa
     6481 gtcagataat tgctttcttt gactggacaa tcattccttt tattccacgt ttcgcttatc
     6541 ctagctgaag cgtttcagtc gattaaatgt tcgacaatta accaatcagt cgcagtttgc
     6601 gacaggtaag gtttccccgg acgatttgct ggattactct gtctggcaaa tttgctcagg
     6661 gaaaaccttg caggagatct atgaccgtac gcgtagcgat aaatggcttc ggtcgcatcg
     6721 ggcgtaatgt ggttcgtgct ttgtatgaat ccggacgccg ggcggaaatt accgtggtgg
     6781 caatcaacga actggcggat gctgcgggca tggcgcattt gttgaaatat gacaccagcc
     6841 atggccgttt tgcatgggaa gtacgacagg aacgcgatca actttttgtt ggtgatgacg
     6901 ccatccgcgt attgcatgaa cgttcactgc aatcgctccc ctggcgtgaa cttggcgttg
     6961 atgtagtcct cgactgcacc ggcgtatatg gctcccgcga gcatggcgaa gcgcatattg
     7021 ccgccggggc caaaaaagtg ctcttttcac atcctggcag taacgatctc gacgcgaccg
     7081 ttgtttacgg cgtcaatcag gatcaacttc gtgcggaaca ccgcatcgtt tctaacgctt
     7141 cctgtaccac gaattgcata attcccgtca tcaaattgtt agatgatgcg tacggtattg
     7201 agtccggcac tgtgaccaca attcactccg ccatgcacga tcaacaggtt attgatgcat
     7261 accatcctga cctgcgtcgc acccgggcag ccagccagtc gatcattccg gtcgatacta
     7321 aactggccgc tggtatcaca cgattttttc cgcaatttaa cgatcgcttt gaagcgattg
     7381 cggtacgtgt gccaaccata aatgtgacgg caatcgattt aagcgtgacg gtgaagaaac
     7441 ctgtaaaagc caatgaagtc aacctgttgc tgcaaaaagc agcacaaggt gcatttcatg
     7501 gtatagttga ctatacggaa ttgccgttgg tctctgtaga ttttaaccac gatccgcaca
     7561 gtgccattgt cgatggcacc caaacccggg tcagtggcgc acacctgatc aaaacgttgg
     7621 tctggtgcga taacgaatgg ggctttgcta accgaatgct cgacacgacg ttagctatgg
     7681 ctactgttgc tttcaggtaa gacgcaagca gcgtctgcaa aacttttaga atcaacgaga
     7741 ggattcacca tgtctgtaat taagatgacc gatctggatc ttgctgggaa acgtgtattt
     7801 atccgtgcgg atctgaacgt accagtaaaa gacgggaaag taaccagcga cgcgcgtatc
     7861 cgtgcttctc tgccgaccat cgaactggcc ctgaaacaag gcgcaaaagt gatggtaact
     7921 tcccacctgg gtcgtcctac cgaaggcgag tacaacgaag aattctctct gctgccggtt
     7981 gttaactacc tgaaagacaa actgtctaac ccggttcgtc tggttaaaga ttacctcgac
     8041 ggcgttgacg ttgctgaagg tgaactggtt gttctggaaa acgttcgctt caacaaaggc
     8101 gagaagaaag acgacgaaac cctgtccaaa aaatacgctg cactgtgtga cgtgttcgta
     8161 atggacgcat tcggtactgc tcaccgcgcg caggcttcta ctcacggtat cggtaaattc
     8221 gctgacgttg cgtgcgcagg cccgctgctg gcagctgaac tggacgcgct gggtaaagca
     8281 ctgaaagaac ctgctcgccc gatggtggct atcgttggtg gttctaaagt atctaccaaa
     8341 ctgaccgttc tggactccct gtctaaaatc gctgaccagc tgattgttgg tggtggtatc
     8401 gctaacacct ttatcgcggc acaaggccac gatgtgggta aatccctgta cgaagctgac
     8461 ctggttgacg aagctaaacg tctgctgacc acctgcaaca tcccggttcc gtctgatgtt
     8521 cgcgtagcaa ccgagttctc tgaaactgca ccggctaccc tgaaatctgt taacgatgtg
     8581 aaagctgacg agcagatcct ggatatcggt gatgcttccg ctcaggaact ggctgaaatc
     8641 ctgaagaatg cgaaaaccat tctgtggaac ggtccggttg gcgtgttcga attcccgaac
     8701 ttccgcaaag gtactgaaat cgtggctaac gctatcgcag acagcgaagc gttctccatc
     8761 gctggcggcg gcgacactct ggcagcaatc gacctgttcg gcattgctga caaaatctcc
     8821 tacatctcca ctggcggcgg cgcattcctc gaattcgtgg aaggtaaagt actgcctgca
     8881 gtagcgatgc tcgaagagcg cgctaagaag taaaaaatca cagggcaggg aaacctgcac
     8941 ttgtttcagc gcgcttttag agcacgcatt ttttcatact ctaaataatt cgagttgcag
     9001 gaaggcgaca agagagtgaa tccccaggag cttacataag taagtgactg gggtgagcga
     9061 acgcagacgc agcacatgca acttgaagta ttatgagtat aaggcccgac gatacaggac
     9121 aagagacatg tctaagattt ttgatttcgt aaaacctggc gtaatcactg gtgatgacgt
     9181 acagaaagtt ttccaggtag caaaagaaaa caacttcgca ctgccagcag taaactgcgt
     9241 cggtactgac tccatcaacg ccgtactgga aactgctgct aaagttaaag cgccggttat
     9301 cgttcagttc tccaacggtg gtgcttcctt tatcgctggt aaaggcgtga aatctgacgt
     9361 tccgcagggt gctgctatcc tgggcgcgat ctctggtgcg catcacgttc accagatggc
     9421 tgaacattat ggtgttccgg ttatcctgca cactgaccac tgcgcgaaga aactgctgcc
     9481 gtggatcgac ggtctgctgg acgcgggtga aaaacacttc gcagctaccg gtaagccgct
     9541 gttctcttct cacatgatcg acctgtctga agaatctctg caagagaaca tcgaaatctg
     9601 ctctaaatac ctggagcgta tgtccaaaat cggcatgact ctggaaatcg aactgggttg
     9661 caccggtggt gaagaagacg gcgtggacaa cagccacatg gacgcttctg cactgtacac
     9721 ccagccggaa gacgttgatt acgcatacac cgagctgagc aaaatcagcc cgcgtttcac
     9781 catcgctgcg tccttcggta acgtacacgg tgtttacaag ccgggtaacg tggttctgac
     9841 tccgaccatc ctgcgtgatt ctcaggaata tgtttctaag aaacacaacc tgccgcacaa
     9901 cagcctgaac ttcgtattcc acggtggttc cggttctact gctcaggaaa tcaaagactc
     9961 cgtaagctac ggcgtagtga aaatgaacat cgataccgat acccaatggg caacctggga
    10021 aggcgttctg aactactaca aagcgaacga agcttatctg cagggtcagc tgggtaaccc
    10081 gaaaggcgaa gatcagccga acaagaaata ctacgatccg cgcgtatggc tgcgtgccgg
    10141 tcagacttcg atgatcgctc gtctggaaaa agcattccag gaactgaacg cgatcgacgt
    10201 tctgtaagtc attcctttct gcttatctca aggcccgctc tgcgggtctt tttttcgcca
    10261 aaagcaaggt gattcagatg agaaatctgt gatctatttg gcaaaattat gctttattgt
    10321 ttacccttgt cagactgccc gtcataaggc ggcggagtgt atttctccat tttgagtcag
    10381 ttgaaaagga atattgaatg gaagatttga atgttgtcga tagcataaac ggcgcgggaa
    10441 gctggctggt agctaaccag gcgctgctgc taagttatgc agtaaacatc gtggcggcac
    10501 tcgcgatcat catcgttggt ttgattatcg cgcggatgat ttccaacgcg gtgaatcgcc
    10561 tgatgatctc ccgtaaaatc gatgccactg ttgctgattt tctttctgca ttagtccgtt
    10621 acggtattat cgcctttacg ctaatcgctg ccctgggacg cgtgggtgta caaacagcgt
    10681 cagtcattgc tgtactcggt gccgcaggct tagctgttgg tctggctttg caggggtcac
    10741 tttctaacct ggccgctggc gtgttacttg tcatgttccg cccgttccgt gccggagaat
    10801 atgttgacct cggcggcgta gccggtactg tgctgagtgt gcagattttc tccaccacca
    10861 tgcgtactgc agacggtaaa attatcgtta ttccgaacgg taaaattatt gccggaaata
    10921 ttattaactt ctcccgcgag ccagttcgcc gtaacgaatt tattattggc gtggcgtatg
    10981 attccgatat cgatcaggtt aagcagatcc tgaccaatat tatccagtct gaagatcgca
    11041 ttttgaaaga tcgcgaaatg actgtgcgcc tgaacgaact tggtgcatcg tcgattaatt
    11101 tcgtggtccg cgtctggagc aacagcggcg atctgcaaaa cgtgtactgg gatgtgctgg
    11161 agcgtattaa acgtgaattt gatgccgccg gtatcagctt cccgtacccg caaatggatg
    11221 tgaactttaa gcgggtgaaa gaagacaaag ctgcgtaatc aacgctgagg ccagataata
    11281 ctctctggcc tctctcttat tagttattct gattgccaat taatattatc aatttccgct
    11341 aataacaatc ccgcgatata gtctctgcat cagatactta attcggaata tccaacgtgt
    11401 tttcttatta ctttcaaggt cttgctcttg gggcagctat gattctgccg ctcggcccac
    11461 aaaatgcttt tgtgatgaat cagggcattc gtcgtcagta ccacattatg attgccttac
    11521 tttgcgctat cagcgatttg gtcctgattt gcgcggggat ttttggtggt agcgcgttat
    11581 tgatgcagtc gccgtggttg ctagcgctgg tcacctgggg cggcgtagtc ttcttgctct
    11641 ggtatggttt tggcgctttt aaaacagcga tgagtagtaa tattgagtta gctagtgccg
    11701 aagtcctgaa gcaaggcaga tggaaaatta tcgccaccat gttggcagtg acctggctga
    11761 atccgcatgt ttacctggat acttttgttg tactgggcag ccttggcggg cagcttgacg
    11821 tcgaaccaaa acgttggttt gcgctcggga caattagcgc ctctttcctg tggttctttg
    11881 gtctggctat tctcgcagcc tggctggcac cgcgcctgcg cacggcaaaa tcacagcgca
    11941 ttatcaatct ggtagtggga tgtgtcatgt ggtttatagc cttgcagctg gcgagagacg
    12001 gtattgctca tgcacaagcc ttgttcagtt aggcgctatc tgatggaaaa ataaaacaga
    12061 ggcgctaagc ttgcctccag aggtcctgaa ttttgggcaa gtcaattaac acggagagac
    12121 taacgtgaag ttcaaagtta tcgccctggc ggcattaatg ggtattagcg ggatggcagc
    12181 gcaggctaac gaattgccgg atggaccgca tattgtcacc tccggtacgg caagtgtgga
    12241 tgcggtgcca gacattgcca ctcttgcgat tgaagttaac gtggccgcga aggatgccgc
    12301 tacagccaag aaacaggcag atgagcgcgt cgcacaatac atttccttcc ttgaactcaa
    12361 tcagatcgcg aaaaaagata tcagctcagc gaatttacgc acccagccag attatgacta
    12421 tcaggatggt aaaagtatcc tgaaaggcta ccgagctgtg agaacggtgg aagtcacgct
    12481 ccgtcagtta gacaaactga attccttgct ggatggcgcg ctgaaggcgg gtcttaacga
    12541 aattcgttct gtgtcgctgg gcgtggcgca gccggatgcc tataaagaca aagcgcgtaa
    12601 ggcagcgatt gataacgcga ttcatcaggc gcaggaactg gcgaacggct ttcatcgtaa
    12661 actggggccg gtatatagcg tgcgctacca tgtttccaac tatcagccca gcccaatggt
    12721 gcggatgatg aaagccgatg ccgcgccggt gtctgcccag gaaacttacg agcaggccgc
    12781 tattcagttt gatgatcagg tcgatgtggt cttccagtta gaacctgtgg atcaacaacc
    12841 cgctaaaaca cctgcagcac aataatactt gtagcccgac gtattcgtcg ggctaatgcc
    12901 tttacccgat attgcctcct ttttctgtaa ttttctgtaa ttttctgtaa aaccatgacg
    12961 taatcaccta tctttgctcg cggtttcatc aaggataggc tgactgaaac ggttaagcgg
    13021 acaaggatgc ggggaggggg gatggacatt tttatttcga aaaagatgcg caatttcatt
    13081 ttattagcgc aaaccaataa tattgcccgg gcggcagaaa aaatccatat gacggcttcg
    13141 ccatttggta aaagcattgc cgcgctggaa gaacaaattg gctatacgct atttacccgc
    13201 aaagataata atatcagcct caataaagcc ggtcaggaac tgtatcaaaa actgttcccg
    13261 gtttatcaac gactttctgc gattgataat gaaatccata actcagggcg tcgttcacga
    13321 gaaattgtga ttggcataga taatacttat ccgacaatta ttttcgatca gttgattagt
    13381 ctcggagata agtatgaagg cgtgaccgcc cagccagttg agttcagcga aaacggcgtt
    13441 atcgataatc tttttgaccg tcagttagat tttattatct ccccacagca tgtatctgca
    13501 cgagttcagg aactggaaaa cctgactatc agtgaattac caccgctgcg attgggcttt
    13561 ctcgtttctc gccgttatga agagcgtcaa gagcaagagc tgcttcagga gctgccctgg
    13621 ctacaaatgc gctttcaaaa ccgggctaac ttcgaagcaa tgattgatgc gaatatgcga
    13681 ccttgtggga tcaatcccac cattatttat cgtccctaca gttttatggc caaaatcagc
    13741 gccgtggagc gcggacattt tctgacggtg atcccgcatt ttgcctggcg actggtgaat
    13801 ccggcaacgc tgaaatattt tgatgcgccg cacaggccga tgtatatgca ggaatatctt
    13861 tattcaatca gaaatcatcg ttataccgcc acgatcttca gcatattgct gaagatcgtg
    13921 acgggacaaa ccattaaccc agcatcgagc cggttgcaat taaattacgg tgtaagtcga
    13981 agacgtggct aagatcgtgg tgaatatgtc cgccgggcgc attttccaga tagcgatgca
    14041 gataatcccg atacatagga tgtgcacaat tatcaatgat agtgcgggcg cgttgaagcg
    14101 gggaaagacc gcgcagatcg gcgatccctt gttcggtgat gatcactttg acgctgtgtt
    14161 cgctgtgatc aacatggctg cacattggca cgacggttga gatcttgcct tctttagcaa
    14221 tcgacggagc cataaagatc gacagatacg cgttgcgttc aaaatcaccg ctgccgccga
    14281 tgccgttcat cagatcgacc ccggctacgt gcgttgaatt tgcatgcccg taaatatcaa
    14341 actccaggcc gacgttcaga gcgatgacgc ccagacgacg gatgatttcc gggttattgg
    14401 aaatctcctg cggacgcaac acaatgcggc tggcaaagta atccatattg tcgtaaatct
    14461 tgcgcaggga atcggccgag attgtcaggc tggaggcgct ggccccgctg attttgccgg
    14521 tttccagtaa atgcaccacc gattcctgta gcacttccga atacatcatg aacggaggaa
    14581 tttccgggtt ttcccccaga cgtgccatta ccgcattatt gatattgccc acgccacttt
    14641 gcagcggcag aaattccggc ggaatacgcc catgcgccat ttcctgcaat aagaacgtga
    14701 ccacgttatc ggcaatctgc tggcacatgg gattttgctt atccagcata ttaccggcgt
    14761 cgggcaagtt ggtttccacg acggcgacaa tctttttcgg atcgatttgc acatagcggg
    14821 taccgacgcg atccattgca tggaagatcg acacgctatt gcgccgcggt ggcgcgccag
    14881 gaatcacaat atctgccagt tctgcaacgc gcggatcgtg atagtgatta agttcaatga
    14941 tcactttctt cgctcgcagc agctaggtcg gcgcattacc gatcccgctg gttaaccaga
    15001 ctcgaccatc cggtgccagt gccgatgctt caatgacggc aacatcaatg tcgccgaaga
    15061 aaccgtaatt gaccatttgc gccacttcgc tcaaatgcag gtcaacgaaa ctcaccgcgc
    15121 cctgattgat ctttttacgt aaactggacg atgtttgata tggcgcacgc caggaaacag
    15181 catcggcgtc agaaagtaca tcgtcagcgg cggcgctgat tgacgccccc gtcagcaggc
    15241 gaatttgata cggctttttg gcctcatgct gttcgttagc tctgcgggca atcgcggcgg
    15301 gtagggcttt cggcgaaccc gctggggtaa agccgctaaa tgccaccatg tcgttatgct
    15361 ggataatttc tgccgcttca tcggcggtca tccttgtcca ctgagtttcc atttttactc
    15421 cttcgttcgc agggattaat gaccaacgaa attaggttta cgtttttcga ggaaagcgtt
    15481 catcccttcc tggtaatctt cgctgtcata aaccgcgcgg cgcatcccct gaatacgttc
    15541 aaattcatcg gagttcatgg tgtgtgcttc gcccagtaca cgcagctctt ctttgataac
    15601 ggcaatggct aacggcgctt tctcagagat gtggtgcgcc atttgtaagg tgaaatcttc
    15661 cagttcttcc acttccacaa catggttgag gatgccgaca gccagcgcgc gctgggcggt
    15721 gattggcgaa gcggtaaaaa tcagctcttt gacaatgtgg aagcccgcgt cgcgggtcag
    15781 gttgtgaatg ccgaccaggt tatacgggac gccgaggttt acaggcgtca ttgagaaggt
    15841 tgaggtactg gcggcgatga tcagatcgga actcatgatc atttcaaatg cgccacccca
    15901 aacactacct tccaccatcg aaatgatcgg tttcgggaat ttttggatca tgcgggtgat
    15961 ttgacgcaat ggatcatcat aggagagcgg atcgcgaccg ccagacggca gttcgtgaat
    16021 atcgtgacct gcggagaaga ctttggatcc acttggcgcc cgcaaaatga tacagcgaat
    16081 ttccggccgg ttgagatcgc ttaacgcctg cataagatca tcaataaaga ctttacttaa
    16141 ggcattaagt tttcgtccat agttaaactc aatgactgcc actttgttga tagtggcaac
    16201 gttcacatac tgataagaca taaaaattcc tttaatcaaa atattgcgtc tggataaatt
    16261 cactgagctg ccgcaggccg gtgcgcggtg agagcgtatt gtttttgacc gctaaaagcg
    16321 tctggcggta atagcgatcg aaatcttcat tcgcgaacag gtgattcagt acttcttctt
    16381 cggtctgctt acgcagccat tccaccgatt gttgttgccg cacttgttgt aaacgaccac
    16441 tggcagttag cgcggttttg aagtcgatga tggcgtgcca gatctcatcg attccacgtt
    16501 tttccagtgc gctacaagtc agaccccgtg gctgccattc gtcgtattta cgtcgcagaa
    16561 tatgcagggc actctcgtac atatgccggg caatggcgac attggtatgg ttatcgccat
    16621 cgtctttgtt gataacgagc agatcagcca cttccatcag ccctttttta atgccctgca
    16681 gatcatcgcc gccaccggca atttgcaacg agataaaaca gtccaccatg cgggcgactt
    16741 ccgtttccga ctgcgcgatg ccaaccgttt cgacaatcac tacgtcataa cccgccgctt
    16801 cgcataacag cattaattcc cgtgctcgct gactggcacc gcccagatga ccggaggagg
    16861 gtaccgggcg aataaacgcc gcctcggcac gcgccaggtc attcatgcgg gttttatccc
    16921 cgagaatgct accgccagtg accgggctgc tgggatcgac cgcaataaca gcaaccttta
    16981 atccctcacg aatcaacaac atgccaaagg cttcaagaaa ggtacttttc cccgcgccgg
    17041 gtgtgccggt aacgcccagt cgcagggcgt taccacagaa cggcataata gcgtcaagca
    17101 gctgtgtact tagcgcctga tgacgtgggt gacggctttc caccagcgtc atggcttggg
    17161 cgagcgtggc acgctcaccc tgacgtaagc ggcgaatact ttctgccagc gtggcttcat
    17221 taatcatgat gctggcttat cagattcagt acgtcgcgca cactgtcgag cataggtgta
    17281 cctggaccat aaatcgccgc cacgccgcgc tcttgcagga aggcgtaatc ctgcggcgga
    17341 atgacgccac ccgcgaccac gcagatatct tcgcgtcccc attttttcag cgcttcgacc
    17401 agttccggga tcagcgtttt atgaccggca gccagtgagg atgcgcccac tacgtgaacg
    17461 tcgttttcta cggctaggcg ggcgatctct tcaggtgtag agaacatcgg gcttaaatct
    17521 acgtcgaaac cgagatcgga ataggcgctg gcgatcactt tcgcgccgcg atcgtgtcca
    17581 tcctggccca tcttagcgat cagaatgcgc gggcgacgac cattgtcggc aaggaactgc
    17641 tccgtttgcg caacaatggc atcgaactcg gaggccgatt tctcagactg atgatagctt
    17701 tgcgcaatca cgccggtaac acactggctt ggcaccagat aacggtcgaa agcgacttcc
    17761 agcgcatcgg aaatttcacc cagggtggcg cgaacgcgag cggcattaac agcggcagcc
    17821 agcaggtttt cgttatgctg tgcggcgtga gtcagggcgt tcaacgcggc ggttacggcg
    17881 gcatcatcac gggtggcgcg aatgcgttcc agcgaagcaa tttgctcgtt acgcaccatc
    17941 acgttgtcga tctcaagtac atcggtttcg tcttcgtgat ccagtttgta cttgttgaca
    18001 ccaacgatga cacgcttgcc ctggtcgatc agcgactgtt cgcgcgctga ggcctcttcg
    18061 atcattcgtt ttggcagacc tgcttcgatc gctttcgcca tgccaccggc ttcgtcgatc
    18121 tgttggataa tagctctggc ttgtttgacg atttgatcgg tcagcgactc aatgtaatag
    18181 gatccggcca gtggatcgac ggtgtggcag agttctgatt cttcctggat gatgatctgg
    18241 gtgttgcggg caatgcgtgc tgagaaatcg gtaggcaaac caagcgcttc gtcaaaggcg
    18301 ttggtatgca gtgactgagt accgcccagc gtcgcagcca gtgcttcaat ggtggtgcgg
    18361 ataacgttgt tatacggatc ctgttcagtc aggctccagc ctgaggtctg gcagtgggta
    18421 cgcagcgcca gtgatttcgg atcctgtgcg ccaaatccac tgaccgcttc gctccataaa
    18481 taacgtgccg cacgcaacat ggcgacgttc ataaacagat ccatgccgat accgaagaat
    18541 aacgacaggc gaggagcgaa gtcatcaatt ttcagtccgg cagagattgc tgctttgatg
    18601 tactcaatcc catcggcgag cgtaaatgct acctgctgca cgcagttggc acccgcttcc
    18661 cccatgtggt aaccgctgat actgatggta ttaaatcgcg gcatgttgcc ggaacaccag
    18721 gcgatgatgt cggcgataat gcgcattgac ggttttggtg ggtaaatata ggtgttgcgg
    18781 cagaggtact ctttgagaat atcgttttga atggtgccgg tcagtttatc aggtgtaaca
    18841 ccttgctctt ctgcggcgac gatataaaac gccagtactg gtagcactgc gccattcatg
    18901 gtcatcgaaa ccgacatttt atccagcggg atctggtcga acaggacttt catatcttcc
    18961 acggtgtcga tagcgacgcc cgctttgccg acgtcacccg ccacgcgtgg gttatcggag
    19021 tcgtagccac ggtgggtggc aaggtcaaac gcaacggaaa gacctttttg cccggcggcc
    19081 aggttacggc gataaaaagc gttggactct tttgctgttg aaaaaccagc atactgacgg
    19141 atggtccacg gttgggcggt atacatagtg gcacgcgggc cacgaacgta gggcggcaaa
    19201 ccaggaaggg tacctgtcac ctccagatta tcgagatcgg cttcggtata cagcggcttg
    19261 atggcgatcc cttccgcggt ttgctgaacc agcgagtcga cagttttctc ccgacggctc
    19321 aattccttgt tggcaagctg ttgccactcc tgctcgttag acattatttg ctccgtaaaa
    19381 cgcaatcaaa atgagggtat ttgcgcttac ggtgagcttt ttacgtgggt ttgtcgccag
    19441 caaaaaaaat gcattcagac gtcgtttttt gccgcctgag atttgtctat tttgtgatgt
    19501 gattcaagga atacaaaaag aagtgaggac aacgcctgat atgtgctatc aggcattatt
    19561 tgatggatta atcctgacga aggactttgt gaccataatc gagtaacgca tcagtgactt
    19621 tacgcatcat gcggctttca ggagcaaagc ggtgccagta gagcatccgt cgttgaaata
    19681 gcccaggcgt taagtcaatc agttcaccgc tggccagctc tttctcgatt tgcaggtgcg
    19741 ggatcataca gcaggtggtg ccctggcgag caagttgtac gaacgcttct gaagaattaa
    19801 cgatatggca gggtacgctg ccaggaggaa gatcgaagtt ttgctgcaaa aaggcctggt
    19861 gcatatcgtc aagatggtca aacgcgacca ctggcgcttt caataatgcc gaacgcgtta
    19921 cgccgttagg aaaatatttt tcggcaaacg gttttgagct gacgaacaga tagtcgagcg
    19981 caccgagttt atcgacaaga caactcggca gcgcctgatg ttgaatactc accgcgccga
    20041 ccacttcgcc gcggcgcaga cgttcctgag tacgggtttc atcttctacc tgcaagttga
    20101 ggcggatagg cgaatcagcc aacacaggag ccagtgccgg aagcaaccac gtcgccagac
    20161 tgtcggcgtt gaccgccagt gaaagcagca gcggagtcga accggtttgt tcatcgccca
    20221 gccactcttc ttccagcaac tccacctggc gcagcagtgc cagcagtttt tgcccttgtt
    20281 ccgtcgggcg cggcggtacg gtacgcacca acagcggctg cccgaacata ttttccagtt
    20341 gcttaatgcg ctgtgagacg gctgattgtg taatgcacag cttttgtgcc gcgcgctcaa
    20401 atcctcgttc acgtatcacc gcatccagtg cctgtaatgt tctgtagtcc gggcgtttca
    20461 ttgctgttgt ctgctcctgg taaatgcttt ttcctgcact atgacacaat tttgtgtcag
    20521 gttgcatata ccgaagccgt cggcgggtaa catttgtatc aatggttaaa cgcgcagctt
    20581 aaaagatcag ggatttgcgc tattgcattt cgcttagcga cttcgcgccc tggttatgtt
    20641 gaatggcgtg gcgttattgc ctctgttcgc accgtggagc ggcataccca gaccgttgta
    20701 tgctttattt gcggggcgaa aggggatgcc cgccattgcg cgatatttta tggatgagtt
    20761 aaccacccgg cttgccaacg gggtctgaat cgcttttttt gtatataatg cgtgtgaaat
    20821 ttcataccac aggcgaaacg atcatgacgc aggatgaatt gaaaaaagca gtaggatggg
    20881 cggcacttca gtatgttcag cccggtacca ttgttggtgt aggtacaggt tccaccgccg
    20941 cacactttat tgacgcgctc ggcacgatga aaggccagat tgaaggggcc gtttccagtt
    21001 cagatgcttc cactgaaaaa ctgaaaagcc tcggcattca cgtttttgat ctcaacgaag
    21061 tcgacagcct tggcatctac gttgatggcg cagatgaaat caacggccac atgcaaatga
    21121 tcaaaggcgg cggcgcggcg ctgacccgtg aaaaaatcat tgcttcggtt gcggaaaaat
    21181 ttatctgtat tgccgacgcc tccaagcagg ttgatattct gggtaaattc ccgctgccag
    21241 tagaagttat cccgatggca cgtagtgcag tggcgcgcca gctggtgaaa ctgggcggtc
    21301 gtccggaata ccgtcagggc gtggtgaccg ataatggcaa cgtgatcctc gacgtccacg
    21361 gcatggaaat ccttgacccg atagcgatgg aaaacgccat aaatgcgatt cctggcgtgg
    21421 tgactgttgg cttgtttgct aaccgtggcg cggacgttgc gctgattggc acacctgacg
    21481 gtgtcaaaac cattgtgaaa tgatctgacg ggggaacctc ccccgttaaa aaaattctct
    21541 tcattaaatt tggtgacatg tgtcacgctt ttaccaggca attgtcgatt gctctaaata
    21601 aatcctctaa accagcatat tcatccaaga attacctttg cgtgatattt cctcaacatc
    21661 gcgacgcaaa cgttcatatt gccgcaatat tattttttga tatgttgaaa ggcggatgca
    21721 aatccgcaca caacatttca aaagacagga ttgggtaaat ggcaaaggta tcgctggaga
    21781 aagacaagat taagtttctg ctggtagaag gcgtgcacca aaaggcgctg gaaagccttc
    21841 gtgcagctgg ttacaccaac atcgaatttc acaaaggcgc gctggatgat gaacaattaa
    21901 aagaatccat ccgcgatgcc cacttcatcg gcctgcgatc ccgtacccat ctgactgaag
    21961 acgtgatcaa cgccgcagaa aaactggtcg ctattggctg tttctgtatc ggaacaaacc
    22021 aggttgatct ggatgcggcg gcaaagcgcg ggatcccggt atttaacgca ccgttctcaa
    22081 atacgcgctc tgttgcggag ctggtgattg gcgaactgct gctgctattg cgcggcgtgc
    22141 cggaagccaa tgctaaagcg caccgtggcg tgtggaacaa actggcggcg ggttcttttg
    22201 aagcgcgcgg caaaaagctg ggtatcatcg gctacggtca tattggtacg caattgggca
    22261 ttctggctga atcgctggga atgtatgttt acttttatga tattgaaaac aaactgccgc
    22321 tgggcaacgc cactcaggta cagcatcttt ctgacctgct gaatatgagc gatgtggtga
    22381 gtctgcatgt accagagaat ccgtccacca aaaatatgat gggcgcgaaa gagatttcgc
    22441 taatgaagcc cggctcgctg ctgattaatg cctcgcgcgg tactgtggtg gatattccgg
    22501 cgctgtgtga tgcgctggcg agcaaacatc tggcgggggc ggcaatcgac gtattcccga
    22561 cggaaccggc gaccaatagc gatccattta cctctccact gtgtgaattc gacaacgtcc
    22621 ttctgacgcc acacattggc ggttcgactc aggaagcgca ggagaatatc ggcttggaag
    22681 ttgcgggtaa attgatcaag tattctgaca atggctcaac gctctctgcg gtgaacttcc
    22741 cggaagtctc gctgccactg catggtggtc gtcgtctgat gcacatccac gaaaaccgtc
    22801 cgggcgtgct aactgcgctg aacaaaatct tcgccgagca gggcgtcaac atcgccgcgc
    22861 aatatctaca aacttccgcc cagatgggtt atgtagttat tgatattgaa gctgacgaag
    22921 acgttgccga aaaagcgctg caggcaatga aagctattcc gggtaccatt cgcgcccgtc
    22981 tgctgtacta attccccttc tctgaaaatt aacgggcagg tcactgactt gcccgttttt
    23041 ttatcccttc tccacaccgt tgccgcgttg cgcattctac tttgcgagtc tgctccagaa
    23101 aataaatcaa agtcagatca atgcgttgtg tttatttaat ggggatttac gatggcggga
    23161 cagcatgggg ctgtaacggt aaagccctca ccgaagcgag ggcttaaagg agaagggtta
    23221 tgatgcgact tgtcatcata ctgattgtac tgttactgat aagtttcgga gcttattaac
    23281 agtcagtctc aagggaggag tcatcctccc ttacccttac tcactaaatt aggtcaaaga
    23341 atcaacgatg tcaatcaggg cgatgcgggg tgcatcgccc tgaaccgaac tttttgatag
    23401 ccacttcatc gcccacgttt actgccgtaa tagatccttt ttcccaaaaa ttatctctgt
    23461 tttgcatggg gtttttcgag cacgctcgaa gaaaagattt aaaggtagat caatgcgttg
    23521 tggttattta aagagagctt acgatagctg ggcagcatgg ggctgtaacg gtaaagccct
    23581 caccgaagcg agggcttgaa ggagaagggt tatgatgcga cttgccatca tactgattgt
    23641 actgttactc ataagtttcc cagcttatta acagtcagtc acaggggagg agcaatcctc
    23701 ccttaccctt actcactaaa ttaggttaaa gaatcaacga tgtcaatcag ggcgatgcag
    23761 gtgtatcgcc cttaccactc ccagactttc gacggtgtaa ccaccgcagg aagagggata
    23821 tcccactctt caacggggag tttttccacc aactgacaat catgcgcata acccaccggt
    23881 tgcgttttat agtgctgcca gttttgtaag gtccgatcat aaaaaccacc gcccattccc
    23941 agacgctgac cgtactcatc aaaggcgacc agcggtgtga tcagcacgtc taatcgggaa
    24001 aggggtagca cgtcacgcac atccagtttt ggctcatgga tcttcaacct gttcatcacc
    24061 agttcgcttt gcggatggta attcaggaac agcaaattac cggcactaaa gggatgcaaa
    24121 actggaagat atacgcgctt accggcgcgc cagagttgtt ctattagcgg ctgggtgtcg
    24181 agttcaccat caaaagagag gaatacagcg accgtatgcg ccatcaccac cgggggataa
    24241 gtcatcatcc gggtagcggc ttgttgaccc atttccagct gttgttccgg cgttaacgca
    24301 cgacgacgtt gccgaatcat tttgcggatt tcttgtcggg ataatgtcag tgggagctct
    24361 ggtagttgcg tcatggctgg tgccagataa gaagggaatc tccgagatgc cgccgcaggc
    24421 tgtaaccctt gaacccttgg ttcaaggtga atgtgtcgtc gcagttttaa ggcttctcgg
    24481 acgaaccgag catgctcacc aaccgcggag cgccacattc ttgtggtatg aaatatcggc
    24541 tcaggggact ggcccgcttg cgaacatctc agagaaattt tgtcttcacg gttactctac
    24601 cacagtaaac cgaaaagtgt cattcaaagt tttggttagt tttttcggtg atgcgacctt
    24661 gttcaagtaa cgcttgttct atggtctgct gcagcatccg aatacgctgt tccatacttg
    24721 ccgcgtagtc acgagtcttt gctttttctt gcgctaactc atagctgata ttcaatgcgg
    24781 caatgaagac caactgttca gtatttgtga ctctagtgcg ttctttcaga tcttgcaacc
    24841 gttggttcag atcgtccgct gcctgattca acgcatccct ttggtcaggc gggcagttca
    24901 cacgcagtga acggccaaaa atttggatat cgacgggttg tgcagacatg ccaccttcct
    24961 gctgattgac tgcgctgcct tcgtctttag acccatggtc tgcgaagggg cgacactata
    25021 gctaccctga tgagaagaga caagcccttt tctggtccac cagggcccaa agtggtagca
    25081 tatcatgaat attcctccct ttgacgacga atgcttatgt ctatacagaa cgaaatgcct
    25141 ggttacaacg aaatgaacca gtatctgaac caacaaggga cgggtctgac ccctgccgag
    25201 atgcatggtt taatcagtgg gatgatatgt ggcggtaacg atgacagctc atggctgccg
    25261 ctacttcacg acctgacgaa cgaaggcatg gctttcggtc atgagctggc acaggcactg
    25321 cgtaaaatgc actctgccac cagcgatgcc ctgcaggatg acggcttcct ttttcagctt
    25381 tatctgcctg atggcgatga tgtcagcgtt ttcgatcggg ctgatgcact ggcaggttgg
    25441 gtcaatcact tcctgcttgg tcttggcgtt acgcaaccga agctggacaa agtgaccggc
    25501 gaaaccggtg aagccatcga cgatctgcgt aacatcgcgc agctgggcta cgacgaagac
    25561 gaagatcagg aagagcttga aatgtcgctt gaagagatca tcgaatacgt tcgtgttgcc
    25621 gcgctgttat gccacgacac ctttactcat ccgcaaccga ccgcgccaga agtacaaaaa
    25681 ccgactctac actaaaaaca aaaaacgtaa ggagagtgtt atgagtgaga tatcccggca
    25741 agagtttcag cgtcgccgtc aggccctggt ggagcaaatg caacccggca gcgccgcgct
    25801 gatttttgct gcaccagaag taacacgtag cgccgacagc gaatacccct atcgtcagaa
    25861 cagtgacttc tggtacttca ccggctttaa cgaaccggaa gcggtgctgg tgctgattaa
    25921 aagcgatgac actcataacc acagcgttct gtttaaccgc gttcgcgacc tgacggcgga
    25981 gatctggttt ggccgtcgct taggccagga tgccgcgcca gagaaactgg gcgttgaccg
    26041 cgcactggca ttcagcgaaa tcaatcagca actttatcaa ctacttaacg gcctggatgt
    26101 ggtttaccat gcccagggcg aatatgcata tgctgatgaa atcgtgaaca gtgcgctgga
    26161 aaaactgcgt aaaggttcgc ggcaaaatct caccgcaccg gcaacgatga tcgactggcg
    26221 tcctgttgtt catgaaatgc gcctgttcaa atcgccagaa gagattgccg tactccgccg
    26281 cgcgggagaa atcaccgcca tggcacatac acgggcgatg gaaaaatgcc gtccgggaat
    26341 gttcgagtac catctggaag gcgaaattca ccacgaattt aaccgccacg gtgcgcgcta
    26401 tccttcctat aacaccattg taggcagcgg tgaaaacggc tgcattctgc actacaccga
    26461 aaacgagagt gaactgcgcg acggtgacct ggtgttgatt gacgcgggtt gtgaatacaa
    26521 aggttacgcg ggcgatatta cccgcacctt cccggtcaac ggcaaattca cccaggccca
    26581 gcgtgaaatc tacgacattg tgctggagtc tctcgaaacc agcctgcgcc tgtatcgtcc
    26641 gggaacttcc attctggaag tcactggtga agtggtgcgc atcatggtta gcggcctggt
    26701 aaaactcggc atcctgaaag gtaatgttga tgaactgatc gctcagaacg cccatcgtcc
    26761 tttctttatg catggcctta gccactggtt aggactggat gtccatgacg ttggcgttta
    26821 tggtcaggat cgctcgcgca ttctggaacc ggggatggta ctgaccgtag agcctggtct
    26881 gtatatcgcg ccggatgcgg atgtgccaga acaatatcgc ggtatcggca ttcgtattga
    26941 agacgacatt gtgattaccg aaaccggcaa cgaaaacctc accgccagcg tggtgaaaaa
    27001 gccggaagaa atcgaagcgt tgatggctgc tgcgagaaag caatgagcgt aatcatcgtc
    27061 ggtggcggca tggcgggcgc gacgctggcg ctggctattt cccggttaag tcacggggcg
    27121 ctgccggtac atttgattga agcgactgcg ccagagtcac atgctcatcc gggctttgat
    27181 ggacgagcga tagcgctggc ggcgggtacc tgtcagcaac tggcgcgcat cggcgtctgg
    27241 caatctctgg ctgattgcgc aaccgccatc actaccgtgc atgtcagcga tcgtggtcat
    27301 gctggattcg tcaccctcgc cgcagaagat taccaactgg cggcgctggg gcaggttgtc
    27361 gaattgcata atgtcgggca acggctgttt gcattgctgc gaaaagcacc tggcgtaatg
    27421 ctgcattgcc ccgatcgcgt agctaacgtt gcccgtactc agagtcatgt tgaagtgacg
    27481 ctggagagcg gcgagatgct gacgggccgc gtgctgatag ccgctgatgg cacccattca
    27541 gcgttagcca ccgcgtgcgg cgttgattgg cagcaggagc cttacgaaca actggcagtg
    27601 attgccaacg ttgctacttc tgttgcgcac gaaggccgcg cttttgaacg cttcacgcaa
    27661 catggcccgc tggcgatgtt gccgatgtct gacggacgct gttcgctggt ctggtgtcat
    27721 ccactggaac ggcgcgaaga ggtgctgtcg tggagcgacg agaagttttg ccgtgaactc
    27781 cagtctgcct ttggctggcg actggggcaa attacccacg ccggtaaacg cagtgcttat
    27841 ccgctggcat taacccgcgc cgccagaccg attacccatc gcactgtgct ggtgggcaat
    27901 gcggcgcaaa ctctgcaccc gattgccggg caagggttta acctcggtat gcgagatgtc
    27961 atgagccttg cggaaaccct gactcaggcg catgagcgcg gagaagacat cggtgattac
    28021 ggcatattgt gccgctatca gcagcgtcga cagagcgatc gcgaagcaac catcggcgtc
    28081 acggacagcc tggtacatct ttttgccaac cgttgggcac cgctggttgt cgggcgcaac
    28141 atcggactga tgacgatgga attattcacc ccggcacgcg atgtgctggc gcagcgcacc
    28201 ctcggttggg tggcgcgttg aaggcggttt taaggaataa acaatgcaaa gtgttgatgt
    28261 agccattgtt ggcggcggca tggtggggct ggcggttgcc tgcggcttac aggggagcgg
    28321 cttacgcgtt gccgtactgg agcagcgcgt accggaacct ctggcggtag atgcactacc
    28381 acaactgcgc gtttcggcta tcaatgccgc cagcgaaaaa ttactcaccc gtcttggcgt
    28441 ctggcaggac attctcgctc gtagggccag ttgttatcac ggcatggaag tgtgggacaa
    28501 agacagtttt ggtcacatct cgtttgacga tcaaagcatg ggctatagcc atcttgggca
    28561 catcgttgaa aactcagtga ttcactacgc gctgtggaac aaagcgcagc agtcgtcaga
    28621 tattactctg ttggccccca cagaattaca gcaggtcgcc tggggagaaa atgaaacctt
    28681 cctgacgctg aaagatggca gcatgttaac ggcgcgtctg gtgattggcg cggacggtgc
    28741 taattcctgg ttgcgcaaca aagcagatat tccgctgact ttctgggatt atcagcatca
    28801 cgcgctggta gcgaccattc gcacggaaga accgcatgat gcggtggcgc ggcaggtttt
    28861 ccatggcgaa ggcattctgg cctttttacc gcttagcgat ccgcatcttt gctcgattgt
    28921 ctggtcactg tcgccagagg aagcgcagcg gatgcagcag gcaagtgaag acgaatttaa
    28981 tcgcgcgtta aatatcgctt ttgataatcg ccttggctta tgcaaggttg agagcgcgcg
    29041 tcaggtgttc ccgctgacgg ggcgttatgc gcgccagttt gccgcacacc gtctggcgct
    29101 ggtgggcgac gccgcgcata ccattcaccc actggcgggg cagggcgtta atctcggatt
    29161 tatggacgcc gcagagctga ttgctgaact gaaacggttg catcgtcagg gtaaagacat
    29221 cgggcagtac atttacctgc gccgctatga acgtagccgc aagcacagcg cggcgctgat
    29281 gctggctggt atgcagggtt ttcgcgatct cttttccggc gctaatccgg cgaaaaaact
    29341 gctgcgtgat attggtttga aactggccga cacgcttcct ggcgttaagc cgcaacttat
    29401 ccgccaggca atgggattaa acgatttgcc tgaatggctg cgttaaaaat ttctcctctg
    29461 ttgtttattt gatacccatc acactttcat ctcccggttt tttcgccggg agattttcct
    29521 catttgaaat aaactaattt cacctccgtt ttcgcattat attttctaat accatgattt
    29581 tttgatttag tgttttttga cattttttta gctcttaata ttgtcttatt caaattgact
    29641 ttctcatcac atcatctttg tatagaaatt ggtgtatttt ttggtttttt attctgtcgc
    29701 gatttttgca ttttttaacc ataagctaat gtgatgatca attttacctt atggttaaca
    29761 gtctgtttcg gtggtaagtt caggcaaaag agaacgattg cgttggggac cgggagtggc
    29821 tccgatgctg ggttttgtgg tgataatttc accatgaaaa agttgtcagc cccgcttatt
    29881 caatgaggac aagatggcac aacagactcc tttgtacgaa caacacacgc tttgcggcgc
    29941 tcgcatggtg gatttccacg gctggatgat gccgctgcat tacggttcgc aaatcgacga
    30001 acatcatgcg gtacgtaccg atgccggaat gtttgatgtg tcgcatatga ccatcgtcga
    30061 tctccgcggc agccgcaccc gggagtttct gcgttatctg ctggcgaacg atgtggcgaa
    30121 gctcaccaaa agcggcaaag ccctttactc ggggatgttg aatgcctctg gcggtgtgat
    30181 agatgatctc atcgtctact actttactga agatttcttc cgcctcgttg ttaactccgc
    30241 cacccgcgaa aaagacctct cctggattac ccaacacgct gaacctttcg gcatcgaaat
    30301 taccgttcgt gatgaccttt ccatgattgc cgtgcaaggg ccgaatgctc aggcaaaagc
    30361 tgccacactg tttaatgatg ctcagcgtca ggcggtggaa gggatgaaac cgttctttgg
    30421 cgtgcaggtg ggcgatctgt ttattgccac cactggttat accggtgaag cgggctatga
    30481 aattgcgctg cccaatgaaa aagcggccga tttctggcgt gcgctggtgg aagcgggcgt
    30541 taagccctgc ggtctggggg cgcgtgacac gctgcgtctg gaagcgggca tgaatcttta
    30601 tggtcaggag atggacgaaa ctatttctcc tttagccgcc aacatgggct ggactatcgc
    30661 ctgggaaccg acagatcgtg actttatcgg tcgtgaagcc ctggaagtgc agcgtgagca
    30721 tggtacagaa aaactggttg gtctggtgat gaccgaaaaa ggcgtgctgc gtaatgaact
    30781 gccggtacgc tttaccgatg cgcagggcaa ccagcatgaa ggcattatca ccagcggtac
    30841 tttctccccg acgctgggtt acagcattgc gctggcgcgc gtgccggaag gtattggcga
    30901 aacggcgatt gtgcaaattc gcaaccgtga aatgccggtt aaagtgacaa aacctgtttt
    30961 tgtgcgtaac ggcaaagccg tcgcgtgatt tacttttttg gagattgatt gatgagcaac
    31021 gtaccagcag aactgaaata cagcaaagaa cacgaatggc tgcgtaaaga agccgacggc
    31081 acttacaccg ttggtattac cgaacatgct caggagctgt taggcgatat ggtgtttgtt
    31141 gacctgccgg aagtgggcgc aacggttagc gcgggcgatg actgcgcggt tgccgaatcg
    31201 gtaaaagcgg cgtcagacat ttatgcgcca gtaagcggtg aaatcgtggc ggtaaacgac
    31261 gcactgagcg attccccgga actggtgaac agcgaaccgt atgcaggcgg ctggatcttt
    31321 aaaatcaaag ccagcgatga aagcgaactg gaatcactgc tggatgcgac cgcatacgaa
    31381 gcattgttag aagatgagta acggctttat tcctcttctg cgggagagga tcagggtgag
    31441 gaaaatttat gcctcaccct cactctcttc gtaaggagag aggttcacaa ttcactgcac
    31501 gtttcaggaa ccatcgctca tgacacagac gttaagccag cttgaaaaca gcggcgcttt
    31561 tattgaacgc catatcggac cggacgccgc gcaacagcaa gaaatgctga atgccgttgg
    31621 tgcacaatcg ttaaacgcgc tgaccggcca gattgtgccg aaagatattc agcttgcgac
    31681 accaccgcag gttggcgcac cggcgaccga atacgccgca ctggcagaac tcaaggctat
    31741 tgccagtcgc aataaacgct tcacgtctta catcggcatg ggttacaccg ccgtgcagct
    31801 accgccggtt atcctgcgta acatgctgga aaatccgggc tggtataccg cgtacactcc
    31861 gtatcagcct gaagtctccc agggccgcct tgaagcactg ctcaacttcc agcaggtaac
    31921 gctggatttg actggactgg atatggcctc tgcttctctt ctggacgagg ccaccgctgc
    31981 cgccgaagca atggcgatgg cgaaacgcgt cagcaaactg aaaaatgcca accgcttctt
    32041 cgtggcttcc aatgtgcatc cgcaaacgct ggatgtggtc cgtactcgtg ctgaaacctt
    32101 tggttttgaa gtgattgtcg atgacgcgca aaaagtgctc gaccatcagg acgtcttcgg
    32161 cgtgctgtta cagcaggtag gcactaccgg tgaaattcac gactacactg cgcttattag
    32221 cgaactgaaa tcacgcaaaa ttgtggtcag cgttgccgcc gatattatgg cgctggtgct
    32281 gttaactgcg ccgggtaaac agggcgcgga tattgttttt ggttcggcgc aacgcttcgg
    32341 cgtgccgatg ggctacggtg gcccacacgc ggcattcttt gcggcgaaag atgaatacaa
    32401 acgctcaatg ccgggccgta ttatcggtgt atcgaaagat gcagctggca ataccgcgct
    32461 gcgcatggcg atgcagactc gcgagcaaca tattcgtcgt gagaaagcga actccaacat
    32521 ttgtacttcc caggtactgc tggcaaacat cgccagcctg tatgccgttt atcacggccc
    32581 ggttggcctg aaacgtatcg ctaaccgcat tcaccgtctg accgatattc tggcggcggg
    32641 cctgcaacaa aaaggtctga aactgcgcca tgcgcactat ttcgacacct tgtgtgtgga
    32701 agtggccgac aaagcgggcg tactgacgcg tgccgaagcg gctgaaatca acctgcgtag
    32761 cgatatcctg aacgccgtgg ggatcaccct tgatgaaacc accacgcgtg aaaacgtaat
    32821 gcagcttttc agtgtgctgc tgggcgataa ccacgggctg gagatcgaca cgctggacaa
    32881 agacgtggct cacgacagcc gctctatcca gcctgcgatg ctgcgcgacg acgaaatcct
    32941 cacccatccg gtgtttaatc gctatcacag cgaaaccgaa atgatgcgct atatgcactc
    33001 gctggagcgt aaagatctgg cgctgaatca ggcgatgatc ccgctgggtt cctgcaccat
    33061 gaaactgaac gccgccgccg agatgatccc aatcacttgg acggaatttg ccgaactgca
    33121 cccgttttgc ccgccggaac aggctgaagg ttatcagcag atgattgcgc agctggctga
    33181 ctggctggtg aaactgaccg gttacgacgc cgtttgtatg cagccgaact ccggcgcaca
    33241 gggcgaatac gcgggcctgc tggcgattcg tcattatcat gaaagccgca acgaagggca
    33301 tcgcgatatc tgcctgatcc cggcttctgc gcacggaact aaccccgctt ctgcacatat
    33361 ggcaggaatg caggtggtgg ttgtggcgtg tgataaaaac ggcaacatcg atctgactga
    33421 tctgcgcgcg aaagcggaac aggcgggcga taacctctcc tgtatcatgg tgacttatcc
    33481 ttctacccac ggcgtgtatg aagaaacgat ccgtgaagtg tgtgaagtcg tgcatcagtt
    33541 cggcggtcag gtttaccttg atggcgcgaa catgaacgcc caggttggca tcacctcgcc
    33601 gggctttatt ggcgcggacg tttcgcacct caacctgcat aaaactttct gcattccgca
    33661 cggcggtggt ggtccgggta tgggaccgat cggtgtgaaa gcgcatctgg caccgtttgt
    33721 accgggtcat agcgtggtgc aaatcgaagg catgttaacc cgtcagggcg cagtttctgc
    33781 ggcaccgttc ggtagcgcct ctatcctgcc aatcagctgg atgtacatcc gcatgatggg
    33841 cgcagaaggg ctgaaaaaag caagccaggt ggcaatcctc aacgccaact atattgccag
    33901 ccgcctgcag gatgccttcc cggtgctgta taccggtcgc gacggtcgcg tggcgcacga
    33961 atgtattctc gatattcgcc cgctgaaaga agaaaccggc atcagcgagc tggacattgc
    34021 caagcgcctg atcgactacg gtttccacgc gccgacgatg tcgttcccgg tggcgggtac
    34081 gctgatggtt gaaccgactg aatctgaaag caaagtggaa ctggatcgct ttatcgacgc
    34141 gatgctggct atccgcgcag aaattgacca ggtgaaagcc ggtgtctggc cgctggaaga
    34201 taacccgctg gtgaacgcgc cgcacattca gagcgaactg gtcgccgagt gggcgcatcc
    34261 gtacagccgt gaagttgcgg tattcccggc aggcgtggca gacaaatact ggccgacggt
    34321 gaaacgtctg gatgatgttt acggcgaccg taacctgttc tgctcctgcg taccgattag
    34381 cgaataccag taattcactg attcgagtat tttctaaagg cgcttcggcg cctttttagt
    34441 cagatgacaa agtacaaaag tgctcagaca gtcccctcgc ccctttgggg agagggttag
    34501 ggtgagggga acaggccggc actggcgcga atatttaccc tcaccccgac cctctccctg
    34561 aaagggcgag ggggaaaagc gtgccaacat tgaagattga gccagtttgt tagcaatctc
    34621 aaagatacgt caacgaatta atttttctcg gaaaaacaaa tggctatagc acttgtgacc
    34681 ggtggcagtc gcggcatcgg gcgggcaact gcattactgt tggcgcaaga agggtatacg
    34741 gtggcggtta attatcagca aaacctccac gcggcgcagg aagtgatgaa cttaataatg
    34801 caagccggtg gcaaagcatt cgtgctccag gcggatatca gcgacgaaaa ccaggtcgtt
    34861 gcgatgttta cagcaatcga tcagcacgat gaaccgctag cagcgctggt caataacgcc
    34921 gggatcttgt ttacccagtg caccgttgaa aaccttaccg cagagcgaat caaccgagta
    34981 ctttccacca acgtgacggg atattttctc tgctgccgcg aggcggtaaa acgcatggcg
    35041 cttaaaaatg gtggcagtgg cggcgctatc gtcaatgtct cttcggtggc ctcacggttg
    35101 ggttcgccag gggaatatgt tgattacgcg gcatcgaaag gggcgattga ttcgttaacc
    35161 accggactat cgctggaagt cgccgcgcag gggatccgcg ttaactgcgt gcggccaggg
    35221 tttatttata ccgaaatgca cgccagcggc ggcgagcctg gacgcgtcga tcgcgttaag
    35281 tcgaacatcc ccatgcagcg tggtggacag gcagaagagg tcgcgcaggc cattgtctgg
    35341 ctactaagtg ataaagcctc ttacgtcacg ggaagtttta tcgatttggc gggcgggaaa
    35401 taaaacaggg aagttgtctg accggatgca acaagtattg catccggtac ttcatcgact
    35461 taaagcttct cgccgttgct ggcaatcacc tctttgtacc agttaaagct cttcttacgt
    35521 gaacgcgaca tatcgccagt accgtcgtca tgtttattca catagataaa gccgtagcgt
    35581 ttgctgtact gcccggtagt gaatgacacg cagtcgatgc agccccacgg tgtgtagccc
    35641 atcagatcca caccatcgta agtcactgct tttttcatct cttcaatatg ggcgcgcagg
    35701 tagtcaatgc ggtagtcgtc gttgatgctg ccatcttctt ccactttgtc gtaagcgcca
    35761 aaaccgtttt cgacaataaa cagcggcctc tgataacgct catacagttc gcaaagggca
    35821 tagcgcagac ctactggatc aatctgccag ccccagtcag acgctttaac atacgggttc
    35881 ggtacgctgc cttcaaaacc agagatcgca tcgccggtgc cgccttcggc cttcactgcg
    35941 ttggtcatgt aatagctgaa accaagataa tcgcaggtgc cttcgcgcag tacatcgaga
    36001 tcgccgtctt ccattttgat attaaatccg cgacgttccc actcgttcaa tacataggac
    36061 gggtaatagc cgcatagctg cacatcggta aagacgtagc gttcgcgcat cgactcctga
    36121 gcgaacatca catcgtccgg gttacatgag tagggataga gcggcaccat cgccagcata
    36181 cagccgactt tcatctccgg gttaatgcga cgcgcggctt tcactgccag ggcgctggcg
    36241 acaaactggt gatgcagcac ctggtacatc gtctcttccg ggttgtcatg ctcggtatac
    36301 accacgccgg agcagcagta accaaacagc ggcgcacgcc agttacgctg gttgtttatt
    36361 tcgttgaagg tcatccagta tttgacttta tgcttgtagc gttcgaatac cacttccgcg
    36421 aaacggacaa agaaatcaac gaatttacgg ttggtccagc taccgtattg ctgcaccaga
    36481 tgcagcggca tttcaaagtg ggagagggtt atcaccggtt cgatgttgta tttcagcagt
    36541 tcatcgaaca gagagtcgta aaacttcagc ccttcttcgt ttggctgagc ttcatcacct
    36601 tttggaaaaa tacgcgtcca ggcgatggaa gtccgaaaac atttgaagcc catttcggca
    36661 aatagcttga tgtcttcctt gtagtgacca taaaaatcaa cggcttcatg gtttggatag
    36721 tattttcctg gcaagacttc tttggtgatt tcgcgcggca cgccgtgtgc gccacaggtc
    36781 agaacgtcac aaatgctcgg tcctttgccg cctttgttcc agccgccttc gacctgatga
    36841 gcggcaacgg cgccacccca taagaaatct ttcggtaagg tgagtttttt cactatcata
    36901 ggctccagat cagtcaattc ttacaggaaa tagtaccatg cagccaaacg acatcacttt
    36961 tttcaacgtt tccaggatga cattctggct gggcgtaaaa ccatcaccat ccgcgacgag
    37021 tctgaatcgc acttcaaaac gggtgatgtg cttcgtgtcg gacgttttga agatgacggt
    37081 tatttttgca cgattgaagt caccgcaacc tcaaccgtaa cgctggatac actgacggaa
    37141 aaacatgcag agcaggaaaa tatgaccctg cctgaactga aaaaggtcat tgctgacatc
    37201 tatcccggtc agacacaatt ttatatgatt gaatttaaat gtctttaaat tgaactcacg
    37261 cctgttagtt gaaatttcgc atttaatttc atgattttta ggagattagt ctcaaatcag
    37321 aatttatttt agctaacagg tgttcactgg aactattctc agttacgctg gagaggtata
    37381 catgtgcgtg tacgtttctc tggagtaagt tatggttcag aagcccctca ttaagcaggg
    37441 atattcgctg gcagaggaaa ttgccaacag cgtcagtcac ggcattgggc tggtgtttgg
    37501 tatcgttggg ctggtgttgc tactggttca ggcggtggat ctcaatgcca gcgccacagc
    37561 gataaccagt tacagcctct atggcggcag tatgatcctg ctgttcctcg cttcgacgct
    37621 ctatcacgcc attcctcatc aacgggcaaa aatgtggctg aagaaatttg accattgcgc
    37681 tatttacctg ttgattgccg gaacctacac gccgtttttg ctggtggggc tggattctcc
    37741 gttagcgcgc gggttgatga ttgttatctg gagcctagca ttgctgggga ttctgttcaa
    37801 actgaccatc gcgcaccgat tcaaaatttt atctctggtg acttatctgg cgatgggctg
    37861 gctgtcgctg gtggtaattt atgaaatggc agttaagctc gcggcgggca gcgttacctt
    37921 actggcggta ggcggcgtgg tttattcgct cggggtgatt ttctacgtct gcaaacgcat
    37981 tccatacaac catgccatct ggcacggctt cgtgctcggc ggtagtgtgt gccactttct
    38041 ggcgatctat ttgtatattg ggcaggcgta atgggtatgc ctttgtaggc ctgataagac
    38101 gcgtcagcgt cgcatcaggc atcggagcgc aaccgccgga tgcggcgtga acgccttatc
    38161 cgatctacgg gcctcgtccc tgttgtgata aaggcatcaa atcccagaca tgataagacg
    38221 cgtcagcgtc gcatcaggcg ataaatgcct gatgcgatac agagatttac tcttcgagcg
    38281 aatacggcag cggctcgata tgcagtgtat tcgcatcgtc gcgtacgcgg aagatgctgt
    38341 ccggttccat atcgttattc atgacgacct gtaccacgac ctgaccatct tccagtttta
    38401 ccgcagccag caccgtaccg gtacgacgcc agttctcgcc cattttcagc tctaagtctt
    38461 caccagcttc cggcagtcgg ctggcactac ctgccagcaa ccagagcgcg cgtttgttgg
    38521 caccacggaa ttttgctcgc gccaccatct cttgtccggt ataacagcct ttcttaaagc
    38581 tgataccgcc cagcgcctgg agattggtcg cctgtgggat aaactgcccg ctgttggcgg
    38641 catcaatcac cgggaaacca gcttcaatgt ttaatgccag ccactgttgg ctattgttca
    38701 gttccgcttc accgcgcagt ttatcggtca gcatattagc agtagcttca tcggttacga
    38761 tcaggaaacg ttctgccggg tgttcaaacc atagcaaagt ggtcgcgcct tctttgacta
    38821 cctgtttttc tttcgaaggc agttcgctaa agagatttgc cagcgcggcg cgcgcctgaa
    38881 aaccggcaac accaagcagc acacgctcgt cgtctggcgc gatggtcact ttagagaata
    38941 ccgcatattt tttcagttca gtcagctgcg gttcacgcac gctgcgccgt tcaatccatg
    39001 caaagccatc gccgtcgcgg aacagacgta aattgctcca cattttacct ttggcgtcgc
    39061 aatgggcggc gagcaggtgc tgatcttctg ccatctggct gacatctgct gtcacctgac
    39121 cctgcatata tttttcgctg tccgcgccag taatggtggc aagcgcccag tcatcaagcg
    39181 tcatcagcgt cagcggtaaa cgggcagaag ccgtaggctg acgtggagga aaaggtgtaa
    39241 aagccataat agagtcctga gttgcttaac ggcgtattaa tgactaatgg taaaagagct
    39301 atgaggcaat gcaagtgctt tcactacgcg atctgtgcct tctgcgaggc gactgccaaa
    39361 aaaatgaagc ccttgaattg ttgtgaatta atttcgcgat cgcgatggtg tttactgata
    39421 ttcccgtaaa agaagtcgaa aaacacgtta caatagccgg gtactggatt ttcgtgagac
    39481 aggaagaaga tggacattaa caacaaagcc cgaattcatt gggcatgccg ccgtggtatg
    39541 cgcgaactcg atatttcaat catgtcgttt ttcgaacatg agtacgacag cttaagcgat
    39601 gacgaaaaac gcatctttat tcgtctgctg gaatgtgacg atccggacct gtttaactgg
    39661 ctgatgaatc acggtaaacc ggccgatgca gaactggaaa taatggtccg actcatccag
    39721 acacggaacc gggaacgtgg tcctgtggca atctgatttg cgcgtctcct ggcgcgcaca
    39781 gtggctttcc ttgctgattc atgggctggt tgccgctgtt attttactca tgccctggcc
    39841 gctcagttac accccgttat ggatggtgtt actttcgctg gtggtatttg attgcgttcg
    39901 cagccagcgg tgtattaatg ctcgccaggg ggaaattcgc ttgttgatgg acgggcgttt
    39961 gcgttggcaa gggcaggagt ggagcatcgt caaagcaccg tggatgatta agagcggcat
    40021 gatgctgcgt ttacgttctg atggcggtaa acggcaacat ttatggctgg cagccgacag
    40081 catggacgaa gccgaatggc gggatttacg gcggattttg ttgcaacaag agacgcaaag
    40141 ataaagatga cggcaaagca tatgcgctaa gccgtcagtg agatcaggcg taatgctctg
    40201 ccatttcgtt gaggatttgc tcgcaccagc tctgaatacg ctcgtcgcta aggtcatact
    40261 ggttagtttc atccagcgcc agacccacga acagttgccc gtcagcaatc accggtttcg
    40321 ggctggtaaa ttcatatcct tccgttggcc agtagccgac gaacttcacg cctttggtcg
    40381 agagtttgtc atgcagcata ccgagcgcat cgaggaacca ctcgccgtat cccagttgat
    40441 cgccaagccc atacagcgca ataattttac cttcaaggtt caggtcgtcg agctgatccc
    40501 agacggcttc ccagtcttcc tggatttcac caaaatccca ggtcgggata cccagaatga
    40561 gcacatcgta ctgctccatt aatttcgggg agtcgtcctt gaggttatgt aaggtcacca
    40621 gttctgggcc gataatatcg cggatttttt ctgccgccat ttcggtgtaa caggtgctgg
    40681 aaccgtaaaa aagacccata ttcataacgt aagcttctcg attctgctgt ggcttttgca
    40741 gctagtgtat cagaatcgct ttagtcacgg cacaataagg cataatggat caaatgagtg
    40801 accataaggg gcgcaagtga aacaggagct ggcacgcatc gagcagtttc ttgatgctct
    40861 gtggctggaa aaaaatctgg ctgaaaatac gttgaacgct taccgtcgcg atctgtcaat
    40921 gatggtggag tggttgcatc accgcgggtt gacgctggcg acggcgcaaa gtgacgattt
    40981 gcaggcatta ctggcagaac ggctggaggg cgggtataaa gccaccagct cagcgcgttt
    41041 gctgagtgca gtgcgccgat tgttccagta tctttatcgc gaaaagtttc gtgaagacga
    41101 tcccagtgcg catctcgctt caccgaaatt gccccagcgt ttgccaaaag atttaagcga
    41161 agcgcaggtc gaacgtttat tacaggcgcc attaattgat cagccactgg agctacgcga
    41221 taaagccatg cttgaagtgt tgtatgctac cgggctgcgt gtctctgaac tggtcgggct
    41281 gacaatgagt gatatcagcc tgcgtcaggg cgtagtgcgg gtcattggta aaggcaacaa
    41341 agagcgcctg gtgccgttag gggaagaggc ggtttactgg ctggaaacct atctggaaca
    41401 tgggcgtccg tggctgctga atggtgtgtc aattgacgtg ttgtttccca gccagcgtgc
    41461 gcagcagatg acgcgacaga ccttctggca ccgcattaaa cattatgccg tgctggcggg
    41521 tatcgacagc gaaaagctgt caccgcatgt gttgcgtcac gcttttgcca ctcatttatt
    41581 aaatcatggt gcggatttac gcgtggtgca gatgctactg ggccacagcg atctttccac
    41641 cacgcaaatt tatacgcatg tcgctaccga gcgtctgcgg caacttcatc aacagcatca
    41701 cccgcgggcg tgatgtctga aaagaacggg aagatttatg aagaaaggtt ttatgttgtt
    41761 tactttgtta gcggcgtttt caggctttgc tcaggctgat gacgcggcaa ttcaacaaac
    41821 gttagccaaa atgggcatca aaagcagcga tattcagccc gcgcccgtag ctggcatgaa
    41881 gacagttctg actaacagcg gtgtgttgta catcaccgat gatggtaaac atatcattca
    41941 ggggccaatg tatgacgtta gtggcacggc tccggtcaat gtcaccaata agatgctgtt
    42001 aaagcagttg aatgcgcttg aaaaagagat gatcgtttat aaagcgccgc aggaaaaaca
    42061 cgtcatcacc gtgtttactg atattacctg tggttactgc cacaaactgc atgagcaaat
    42121 ggcagactat aacgcgctgg ggatcaccgt gcgttatctt gctttcccgc gccaggggct
    42181 ggacagcgat gcagagaaag aaatgaaagc tatctggtgt gcgaaagata aaaacaaagc
    42241 gtttgatgat gtgatggcag gtaaaagcgt cgcaccagcc agctgcgacg tggatattgc
    42301 cgaccattac gcacttggcg tccagcttgg cgttagcggt actccggcag ttgtgctgag
    42361 caatggcaca cttgttccgg gttaccagcc gccgaaagac atgaaagaat tcctcgacga
    42421 acaccaaaaa atgaccagcg gtaaataatt cgcgtgaaac aacagataca acttcgtcgc
    42481 cgtgaagtcg atgaaacggc agacttgccc gctgaattgc ctcccttgct gcgccgttta
    42541 tacgccagcc ggggagtacg cagtgcgcaa gaactggaac gcagtgttaa aggtatgttg
    42601 ccctggcagc aactaagcgg tgtcgaaaag gccgttgaga tcctttacaa cgcttttcgc
    42661 gaaggaacgc ggattattgt ggtcggtgat ttcgacgccg acggcgcgac cagcacggct
    42721 ctaagcgtgc tggcgatgcg ctcgcttggt tgcagcaata tcgactacct ggtaccaaac
    42781 cgtttcgaag acggttacgg cttaagcccg gaagtggtcg atcaggccca tgcccgtggc
    42841 gcgcagttaa ttgtcacggt ggataacggt atttcctccc atgcgggagt tgagcacgct
    42901 cgctcgttgg gcatcccggt tattgttacc gatcaccatt tgccgggcga cacattaccc
    42961 gcagcggaag cgatcattaa ccctaacttg cgcgactgta atttcccgtc gaaatcactg
    43021 gcaggcgtgg gtgtggcgtt ttatctgatg ctggcgctgc gcaccttttt gcgcgatcag
    43081 ggctggtttg atgagcgcgg catcgcaatt cctaacctgg cagaactgct ggatctggta
    43141 gcactgggga cagtagcgga cgtcgtgccg ctggacgcta ataatcgcat tctgacctgg
    43201 caggggatga gtcgcatccg tgccggaaag tgccgtccag ggattaaagc gctgctggaa
    43261 gtggcaaatc gcgatgcaca aaaactagcc gccagcgatt taggttttgc gctgggaccg
    43321 cgtctcaatg ctgccgggcg actggatgat atgtccgttg gtgtggcgct gttgctgtgc
    43381 gacaacatcg gcgaagcgcg cgtgctggca aatgaactcg atgcgctaaa ccagacgcga
    43441 aaagagatcg aacaaggaat gcaggttgaa gccctgaccc tgtgcgagaa actggagcgc
    43501 agccgtgaca cgctacccgg cgggctggca atgtatcacc ccgaatggca tcagggcgtt
    43561 gtcggtattc tggcttcgcg tatcaaagag cgttttcacc gtccggttat cgcctttgcg
    43621 ccagcaggtg acggtacgct gaaaggttcc ggtcgctcca ttcaggggct gcatatgcgt
    43681 gatgcgctgg agcgattaga cacactctac cccggcatga tgctcaagtt tggcggtcat
    43741 gcgatggcgg cgggtttgtc gctggaagag gataaattcg aactctttca acaacggttt
    43801 ggcgaactgg ttactgagtg gctggcccct tcgctattgc aaggcgaagt ggtgtcagac
    43861 ggtccgttaa gcccggccga aatgaccatg gaagtggcgc agctgctgcg cgatgctggc
    43921 ccgtgggggc agatgttccc ggagccgctg tttgacggtc atttccgtct gctgcaacag
    43981 cggctggtgg gcgaacgtca tttgaaggtg atggtcgaac cggtcggcgg cggtccactg
    44041 ctggatggta ttgcttttaa tgtcgatacc gctctctggc cggataacgg cgtgcgcgaa
    44101 gtgcaactgg cttataagct cgatatcaac gagtttcgcg gcaaccgcag cctgcaaatt
    44161 atcatcgaca atatctggcc aatttagcgt catcttctct ataaaaaaga gcgtggattg
    44221 ggtacaatcc cgctcttatc accgcatttt gactagctca ataaaagaaa tcagaccatg
    44281 tttgaaatta atccggtaaa taatcgcatt caggacctca cggaacgctc cgacgttctt
    44341 agggggtatc tttgactacg acgccaagaa agagcgtctg gaagaagtaa acgccgagct
    44401 ggaacagccg gatgtctgga acgaacccga acgcgcacag gcgctgggta aagagcgttc
    44461 ctccctcgaa gccgttgtcg acaccctcga ccaaatgaaa caggggctgg aagatgtttc
    44521 tggtctgctg gaactggctg tagaagctga cgacgaagaa acctttaacg aagccgttgc
    44581 tgaactcgac gccctggaag aaaaactggc gcagcttgag ttccgccgta tgttctctgg
    44641 cgaatatgac agcgccgact gttacctcga tattcaggcg gggtctggcg gtacggaagc
    44701 acaggactgg gcgagcatgc ttgagcgtat gtatctgcgc tgggcagaat cgcgtggttt
    44761 caaaactgaa atcatcgaag agtcggaagg tgaagtggcg ggtattaaat ccgtgacgat
    44821 caaaatctcc ggcgattacg cttacggctg gctgcgtaca gaaactggcg ttcaccgcct
    44881 ggtgcgtaaa agcccgtttg actccggcgg tcgtcgccac acgtcgttca gctctgcgtt
    44941 tgtttatccg gaagttgatg atgatattga tatcgaaatc aacccggcgg atctgcgcat
    45001 tgacgtttat cgcgcgtccg gcgcgggcgg tcagcacgtt aaccgtaccg aatctgcggt
    45061 gcgtattacc cacatcccga ccgggatcgt gacccagtgc cagaacgacc gttcccagca
    45121 caagaacaaa gatcaggcca tgaagcagat gaaagcgaag ctttatgaac tggagatgca
    45181 gaagaaaaat gccgagaaac aggcgatgga agataacaaa tctgacatcg gctggggcag
    45241 ccagattcgt tcttatgtcc ttgatgactc ccgcattaaa gatctgcgca ccggggtaga
    45301 aacccgcaac acgcaggccg tgctggacgg cagcctggat caatttatcg aagcaagttt
    45361 gaaagcaggg ttatgaggaa ccaacatgtc tgaacaacac gcacagggcg ctgacgcggt
    45421 agtcgatctt aacaatgaac tgaaaacgcg tcgtgagaag ctggcgaacc tgcgtgagca
    45481 gggaattgcc ttcccgaacg atttccgtcg cgatcatacc tctgaccaat tgcacgcaga
    45541 attcgacggt aaagagaacg aagaactgga agcgctgaac atcgaagtcg ccgttgctgg
    45601 ccgcatgatg acccgccgta ttatgggtaa agcgtctttc gttaccctgc aggatgtggg
    45661 tggccgcatt cagctgtacg ttgcccgtga cgatctgccg gaaggcgttt acaacgagca
    45721 gttcaaaaaa tgggacctcg gcgacatcct cggcgcgaaa ggtaaactgt tcaaaaccaa
    45781 aaccggcgaa ctgtctatcc actgtaccga gctgcgtctg ctgaccaaag cactgcgtcc
    45841 gctgccggat aaattccacg gcttgcagga tcaggaagcg cgctatcgtc agcgttatct
    45901 ggatctcatc tctaacgatg aatcccgtaa cacctttaaa gtacgctcgc agatcctctc
    45961 tggtattcgc cagttcatgg tgaaccgcgg ctttatggaa gttgaaacgc cgatgatgca
    46021 ggtgatccca ggcggtgccg ctgcgcgtcc gtttatcacc caccataacg cgctggatct
    46081 cgacatgtac ctgcgtatcg cgccggaact gtacctcaag cgtctggtgg tcggtggctt
    46141 cgagcgtgta ttcgaaatca accgtaactt ccgtaacgaa ggtatttccg tacgtcataa
    46201 cccagagttc accatgatgg aactctacat ggcttacgca gattacaaag atctgatcga
    46261 gctgaccgaa tcgctgttcc gtactctggc acaggatatt ctcggtaaga cggaagtgac
    46321 ctacggcgac gtgacgctgg acttcggtaa accgttcgaa aaactgacca tgcgtgaagc
    46381 gatcaagaaa tatcgcccgg aaaccgacat ggcggatctg gataacttcg actctgcgaa
    46441 agcgattgct gaatctatcg gtatccacgt tgagaagagc tggggtctgg gccgtatcgt
    46501 taccgagatc ttcgaagaag tggcagaagc acatctgatt cagccgacct tcattactga
    46561 atatccggca gaagtttctc cgctggcgcg tcgtaacgac attaacccgg aaatcacaga
    46621 ccgctttgag ttcttcatcg gtggtcgtga aatcggtaac ggctttagcg agctgaatga
    46681 cgcggaagat caggcgcaac gcttcctgga tcaggttgcc gcgaaagacg caggtgacga
    46741 cgaagcgatg ttctacgatg aagattacgt taccgcactg gaacatggct taccgccgac
    46801 agcaggtctg gggattggca tcgaccgtat ggtaatgctg ttcaccaaca gccataccat
    46861 ccgcgacgtt attctgttcc cggcgatgcg tccggtgaaa taagcattac gttatgctca
    46921 caaccccggc aaatgtcggg gttttttatt taagctgggt aaatgcagat aatcgtattc
    46981 tggcttcgcg atttgtcgcc tgcatcacca tccacggact gaacgcccac ggcgtggcat
    47041 caataccgcg taatacatca gctaaatcac accattgata atccatcact tcatcatcgt
    47101 tgatctgtaa cgcactggtg gtgcgtgcgg caaataccgg acacacttca ttttccacaa
    47161 tgcctctcgg atcggtggcg cggtagcgaa agtcaggata gatagattca ggaggcgtaa
    47221 tttccacgcc aagctcataa cggcaacggc ggatcaccgc gtcttcgctg ctttctccca
    47281 gttgtgggtg cccacaaacc gagttagtcc acacgccagg ccacgctttt ttgctcagtg
    47341 cgcggcgggt aactaataat tgccccttgg cattaaacag ccaactggag aacgcgagat
    47401 gtaagagggt gtctgccgtg tgtgcggcat acttttccag cgtacccgtg ggaactccct
    47461 gtgcattcaa taaaatgacg tgttccgttt gcataatttc tcacatgtaa ttctgatcac
    47521 tcatcattat agataattga agagtgaaat gattaaccaa gagagcgttt atcgacaacc
    47581 gcccgttttg agcggatgcc gacaggtaaa aaccatttaa aaaagtagca tcatatattt
    47641 gaagcgactt tcaatggcca ttgcaacaca gtcagaaatg acaggaatag agagagtaaa
    47701 aagcaacaac agactgaata tagcgcgcat taaaaaattc atattattct ccatgctcat
    47761 ttttcttcag cttgcgcatc agaagccaca tacgtactgt cctgacttct aaatctttat
    47821 cagagacgga ttccttcacc aggtcagcat gatgctgata gccattaaag aagacgttca
    47881 gcacaacggc gctaagggtt gcgagcataa tgccgctatg tagcagcggt tgcagtacgg
    47941 ccggtaactt agaaaagaaa tcgtgagaga gcgtcggagt catgccaacg ccgagactga
    48001 tcgccacaat atagaggttg taacggttgg tggtgtagtt acagcgcgac agaattcgaa
    48061 tccctgtcgc cagtaccatg ccgaacatca ctagaccagc gccgcccagc acaaattgcg
    48121 gaatggaggc taccagcacc gccatttttg gcaccatgcc aaacaggatt aatataattc
    48181 ccgaagaaat acacacccag cggctatgaa cgcgcatcac gctaaccagg ccaacgtttt
    48241 gagaaaatga cgtgtggggg aagctgttaa acgtgccgcc tatcattgtc cctacgccat
    48301 cgacacgcag cccgcaaata atatcgtgtg aagagagttt acgaccgact atttcaccca
    48361 gtgccaggaa catccccatc gactcgataa acacgatgat taacacggca gtcatggtca
    48421 gaatggaaac gggatcgaaa atcggcatac caaacgacat cggggtgaca atcgcaaacc
    48481 acgaggcatc atgtagcccg gataaattga cttcattcat catccacgaa agtaaaaagc
    48541 caaatacaat ccccagtaat acggcgacgt tggacataaa tcctttcgca tagcgagtaa
    48601 tgagcaagat aaaaattaag acggcaaagg agatacctaa ataaacggga ttaccatatt
    48661 gcggattccc tttacctcca gcggcccagt caatacccac ctgaatgatg ctaagcccaa
    48721 tagaagtaat aaccacaccg gtaaccagtg gcgggaataa aggcatcaag cgaccaataa
    48781 gtggcgctaa taatgtggtg ataaaacccg cggcgatagt ggcaccaaat atccccagca
    48841 ggccgatatc cgggttcatc ccaatggcta tcattggtgt tacagcagca aaggtcaccg
    48901 acataatcac cggcaggcgg atccccataa agcggccgat accgatacat tgcaataatg
    48961 tgacgatccc gcagcaaaag agatccgagc taatgagcat cgcaatagca tcttttgaga
    49021 ggcccagtcg gtcaccaatc ataagaggaa cagcgactgc acctgcgtac atgacaagga
    49081 cgtgttgcag accgagtatg atcagctttc ctggtgataa tatgcggtca acctcatcag
    49141 ttgggcggtc ttgcccagaa gatgagggaa gttgggaatc tatggcgctc atcagtgttt
    49201 ctcctttcgc ccgccagaat aaaaagcaac cgtttacgtt aatgaaactg tctaaacaca
    49261 tgctcgtgga gtgccagttt ttgcgaggaa ggggagttag agacaggaat tgcggaggca
    49321 atcacataat cgtgcttaaa ccacgaaaat gacaagagaa actgacaatg gaattatctg
    49381 taattttctc atattgatat ttcattaatc attctggcat gtcgcactct cgcatttaat
    49441 cgtttttatc tggatagcgc tcttttgatc ggcaatgttg aaataatgaa agcggcatag
    49501 attgaggggc aataatttga actcagtcgc aaaattataa ttaaaacggt catgctacct
    49561 aatatatgcc cgtttattgc atcgcgaaat ttgagaagac ataactcatt aaatgaaagg
    49621 gatgcaaatg aatacgttta tcgctgctga agctgcggaa tgta
//
LOCUS       DAJTQQ010000021        49531 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000021 DAJTQQ010000000
VERSION     DAJTQQ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.021"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..1150)
                     /gene="setB"
                     /locus_tag="OO309_001329"
     CDS             complement(<1..1150)
                     /gene="setB"
                     /locus_tag="OO309_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR5846528.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIAT"
     gene            1518..2648
                     /gene="fruB"
                     /locus_tag="OO309_001330"
     CDS             1518..2648
                     /gene="fruB"
                     /locus_tag="OO309_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR5846529.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2648..3586
                     /gene="fruK"
                     /locus_tag="OO309_001331"
     CDS             2648..3586
                     /gene="fruK"
                     /locus_tag="OO309_001331"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR5846530.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3603..5294
                     /gene="fruA"
                     /locus_tag="OO309_001332"
     CDS             3603..5294
                     /gene="fruA"
                     /locus_tag="OO309_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR5846531.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5718..6659
                     /locus_tag="OO309_001333"
     CDS             5718..6659
                     /locus_tag="OO309_001333"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR5846532.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6647..7585
                     /gene="psuG"
                     /locus_tag="OO309_001334"
     CDS             6647..7585
                     /gene="psuG"
                     /locus_tag="OO309_001334"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR5846533.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7679..8929
                     /locus_tag="OO309_001335"
     CDS             7679..8929
                     /locus_tag="OO309_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5846534.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9000..9694)
                     /gene="yeiL"
                     /locus_tag="OO309_001336"
                     /pseudo
     CDS             complement(9000..9694)
                     /gene="yeiL"
                     /locus_tag="OO309_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9825..10766
                     /gene="rihB"
                     /locus_tag="OO309_001337"
     CDS             9825..10766
                     /gene="rihB"
                     /locus_tag="OO309_001337"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR5846535.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10866..12116
                     /locus_tag="OO309_001338"
     CDS             10866..12116
                     /locus_tag="OO309_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5846536.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12172..13260)
                     /locus_tag="OO309_001339"
     CDS             complement(12172..13260)
                     /locus_tag="OO309_001339"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5846537.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="OO309_001340"
     CDS             complement(13263..14120)
                     /gene="nfo"
                     /locus_tag="OO309_001340"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR5846538.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14194..15243)
                     /locus_tag="OO309_001341"
     CDS             complement(14194..15243)
                     /locus_tag="OO309_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR5846539.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15342..16223
                     /locus_tag="OO309_001342"
     CDS             15342..16223
                     /locus_tag="OO309_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5846540.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16429..17898
                     /gene="lysP"
                     /locus_tag="OO309_001343"
     CDS             16429..17898
                     /gene="lysP"
                     /locus_tag="OO309_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR5846541.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18190..20181
                     /gene="cirA"
                     /locus_tag="OO309_001344"
                     /pseudo
     CDS             18190..20181
                     /gene="cirA"
                     /locus_tag="OO309_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="OO309_001345"
                     /pseudo
     CDS             complement(20213..21048)
                     /gene="yeiG"
                     /locus_tag="OO309_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21306..21974
                     /gene="folE"
                     /locus_tag="OO309_001346"
     CDS             21306..21974
                     /gene="folE"
                     /locus_tag="OO309_001346"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR5846542.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21991..23067
                     /locus_tag="OO309_001347"
     CDS             21991..23067
                     /locus_tag="OO309_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR5846543.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23209..24249
                     /gene="galS"
                     /locus_tag="OO309_001348"
     CDS             23209..24249
                     /gene="galS"
                     /locus_tag="OO309_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR5846544.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24529..25527
                     /gene="mglB"
                     /locus_tag="OO309_001349"
     CDS             24529..25527
                     /gene="mglB"
                     /locus_tag="OO309_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR5846545.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25588..27108
                     /gene="mglA"
                     /locus_tag="OO309_001350"
     CDS             25588..27108
                     /gene="mglA"
                     /locus_tag="OO309_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR5846546.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27124..28134
                     /gene="mglC"
                     /locus_tag="OO309_001351"
     CDS             27124..28134
                     /gene="mglC"
                     /locus_tag="OO309_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR5846547.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28170..29383)
                     /gene="preA"
                     /locus_tag="OO309_001352"
                     /pseudo
     CDS             complement(28170..29383)
                     /gene="preA"
                     /locus_tag="OO309_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29377..30615)
                     /gene="preT"
                     /locus_tag="OO309_001353"
     CDS             complement(29377..30615)
                     /gene="preT"
                     /locus_tag="OO309_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR5846548.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30809..31048)
                     /locus_tag="OO309_001354"
     CDS             complement(30809..31048)
                     /locus_tag="OO309_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR5846549.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31051..31770)
                     /gene="sanA"
                     /locus_tag="OO309_001355"
     CDS             complement(31051..31770)
                     /gene="sanA"
                     /locus_tag="OO309_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR5846550.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31920..32804)
                     /gene="cdd"
                     /locus_tag="OO309_001356"
     CDS             complement(31920..32804)
                     /gene="cdd"
                     /locus_tag="OO309_001356"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR5846551.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32934..33629)
                     /locus_tag="OO309_001357"
     CDS             complement(32934..33629)
                     /locus_tag="OO309_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR5846552.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33626..34024)
                     /locus_tag="OO309_001358"
     CDS             complement(33626..34024)
                     /locus_tag="OO309_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR5846553.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34263..35210
                     /gene="dusC"
                     /locus_tag="OO309_001359"
     CDS             34263..35210
                     /gene="dusC"
                     /locus_tag="OO309_001359"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR5846554.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35581..35664)
                     /gene="yohP"
                     /locus_tag="OO309_001360"
     CDS             complement(35581..35664)
                     /gene="yohP"
                     /locus_tag="OO309_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR5846555.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35888..37324
                     /gene="mdtQ"
                     /locus_tag="OO309_001361"
                     /pseudo
     CDS             35888..37324
                     /gene="mdtQ"
                     /locus_tag="OO309_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            37377..38138
                     /locus_tag="OO309_001362"
                     /pseudo
     CDS             37377..38138
                     /locus_tag="OO309_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38255..38833)
                     /locus_tag="OO309_001363"
     CDS             complement(38255..38833)
                     /locus_tag="OO309_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5846556.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39003..39590
                     /locus_tag="OO309_001364"
     CDS             39003..39590
                     /locus_tag="OO309_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR5846557.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39764..40696
                     /gene="pbpG"
                     /locus_tag="OO309_001365"
     CDS             39764..40696
                     /gene="pbpG"
                     /locus_tag="OO309_001365"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR5846558.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40734..42449)
                     /gene="dld"
                     /locus_tag="OO309_001366"
     CDS             complement(40734..42449)
                     /gene="dld"
                     /locus_tag="OO309_001366"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5846559.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42645..44942
                     /gene="bglX"
                     /locus_tag="OO309_001367"
     CDS             42645..44942
                     /gene="bglX"
                     /locus_tag="OO309_001367"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR5846560.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45194..46111
                     /gene="osmF"
                     /locus_tag="OO309_001368"
     CDS             45194..46111
                     /gene="osmF"
                     /locus_tag="OO309_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR5846561.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46118..47275
                     /gene="yehY"
                     /locus_tag="OO309_001369"
     CDS             46118..47275
                     /gene="yehY"
                     /locus_tag="OO309_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR5846562.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47268..48194
                     /gene="yehX"
                     /locus_tag="OO309_001370"
     CDS             47268..48194
                     /gene="yehX"
                     /locus_tag="OO309_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR5846563.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48199..48930
                     /gene="yehW"
                     /locus_tag="OO309_001371"
     CDS             48199..48930
                     /gene="yehW"
                     /locus_tag="OO309_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR5846564.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48911..49018)
                     /locus_tag="OO309_001372"
     CDS             complement(48911..49018)
                     /locus_tag="OO309_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR5846565.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49078..49531)
                     /locus_tag="OO309_001373"
                     /pseudo
     CDS             complement(49078..49531)
                     /locus_tag="OO309_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        11957 a        12205 c        13309 g        12060 t
ORIGIN      
        1 gagtggcgat aatcatcacc atcgcaaacc agaacacagc gtgataattc cagatctcgg
       61 cgacgatgcc cgccactgat cctgcgatga tccagcccac gcgcgacgtg ttggtataga
      121 gcgtggtggc tgaacctgcc tgaccgggca tcaaatcctg aaaatagagc atcccgatgc
      181 cgcccagaat gccaataaaa atagcattta gcagctgcaa gcccaacaga atggcaggtg
      241 aatgcgccat cagcattcct gcgtaaaaac agacgccacc cacggcagca acgcgcatta
      301 agaaacgctt acccagacgt ttggcgaaat atccggcaat caacatcgtc gggatttcca
      361 gcccggcggc ggtccccatc atcacaccgg ccagtttctc gggaagatgc agttcgttga
      421 taataaatag cggcatgttg atgatgtaca ggctgttcga gccccacatc aatgtacaaa
      481 tgacaaacag cagtagcgta tcgcgacggt tacggcgcgg cgcttcaacc gtgccggtcg
      541 ccagcggaag ctctttttgc atcgacggta aaaacagcca caccatcacg ccgcaaacaa
      601 taaacgctac cgctgcgctc agatacatta ccgtaaagct gaaacccatc gctaacgcat
      661 aagccagcgg tgggccaatg acccatgcca gtgaaacctg agcgcgtaaa aaagagctga
      721 acatcaccgc ctcacgtccg gttttgtcgg catgttcacg ggcaagggca aacatttgcg
      781 ggttagcggt cgagccaaag ctgctaagaa agacgccaac gaatagcaaa acaaagtagt
      841 tgcgattcca agcaaaaagg gtgcaggcca gcacgcctaa caggcagcaa aagacaatca
      901 gcgatttgcg atcgccgcgc ttatcagagc gcccagcgag aaactgactt accagaatcc
      961 caatgacagc gctgccggta aagaagaatc ccaccatcgc cggacgggca tgtacttcat
     1021 cggtaaggaa aatactgagt gtcggggttt gcagagcacc cgcaataccg gtgagaaagg
     1081 caacgattaa aaacgccgtc gaggtcaggt caaacgattt cgcgctggag actgcggggg
     1141 agttatgcat gctggtcgat cactggaaga ggaagacgcg gagtttacgc tgcttgtcag
     1201 aaaataaaca gacagcgaaa tcaaaagcgc cacgaataat caaaatgcca tttcactgtt
     1261 gctgattggc tgctgacgct gctgaaatga aattgctgat aacggatttt cccatcagca
     1321 attaggaaaa atgacaaaaa attgtgcagc acatcaaact tttgctcata actttacggc
     1381 tttccttgcg tgctgaaaca gaagtattat gctttcttga aacgtttcag cgcgatcttg
     1441 tctttaaccc taagctaggt tggcgctttt tttctcatag aggctgaatc gtttcaattc
     1501 agcaagagag gagaactatg ttccagttat ccgtacagga catccatccg ggcgaaaagg
     1561 ccggagacaa agaagaggcg attcgccagg tcgctgcggc gctggtgcag gccggtaatg
     1621 tagcagaagg ctacgtcaat ggcatgctgg cgcgcgaaca gcaaacctca acgttcctcg
     1681 gcaatggtat tgctattcca cacggcacta ccgacacccg cgatcaggtg ctgaaaaccg
     1741 gcgttcaggt atttcagttc ccggaaggcg tcacctgggg tgacggtcag gtagcgtacg
     1801 tggcaatcgg tattgctgcc agctcggata aacatctggg cctgctacgc cagctgaccc
     1861 acgtactgag cgatgattcc gttgctgaac aactgaagtc agcaacaaca gcagaagaac
     1921 ttcgcgcatt actgatgggc gaaaagcaga gtgagcagct gaagctcgac aacgaaatgc
     1981 tgacgctgga tatcgtcgcc agcgatctgc tgactcttca ggcgctgaac gctgcgcgtc
     2041 tgaaagaggc gagggcagtt gacgccactt tcgtcaccaa agccatcaat gaacaaccgc
     2101 tgaatctcgg acagggtatc tggctgagcg atagcgccga aggcaatctg cgtagtgcga
     2161 ttgcggtaag ccgtgcggca aatgcttttg atgtggacgg cgaaacggca gccatgctgg
     2221 tgagtgtggc gatgaatgac gatcagccca tcgcggttct taagcgtctc gctgatttgt
     2281 tgctcgacaa taaagctgac cgcttgctga aagcggatgc ggcaacgttg ctggcgctgc
     2341 tgaccagcga tgatgcgccg accgacgacg tgttaagcgc ggagtttgtg gtgcgcaatg
     2401 aacacggcct gcatgctcgt ccaggtacca tgctggtcaa taccattaaa caatttaaca
     2461 gtgatattac cgtgacaaac cttgatggca ccggcaaacc ggcaaacgga cgtagtctga
     2521 tgaaagttgt ggcacttggc gttaagaaag gtcatcgcct acgctttacc gcccagggtg
     2581 cagatgctga acaggcgctg aaagccattg gcgacgctat cgctgctggt cttggggagg
     2641 gcgcataatg agcagacgtg ttgctacgat cacccttaat ccggcttatg accttgttgg
     2701 tttctgcccg gaaattgaac gcggcgaagt gaacctggtg aaaaccaccg gtctgcatgc
     2761 ggcgggtaaa ggcatcaacg tggccaaagt attaaaagac ctgggaattg atgtcaccgt
     2821 tggcggcttc ctgggtaaag acaatcagga tggttttcag caactgttca gcgagctggg
     2881 cattgccaac cgtttccagg ttgtacaggg gcgcacccga attaacgtta agctgacgga
     2941 aaaagacggc gaagtgaccg acttcaactt ctcgggtttc gaagtcaccc ccgccgactg
     3001 ggaacgcttt gtgactgatt ctctgagctg gctcggtcag ttcgatatgg tctgtgtcag
     3061 cggaagctta ccgtcaggcg tcagcccgga agcgttcacc gactggatga ctcgcctgcg
     3121 tagtcagtgt ccttgcatta tctttgatag tagccgtgaa gcgttagtag caggtttgaa
     3181 agcggcaccg tggctggtga aacctaaccg ccgcgagttg gaaatctggg caggccgtaa
     3241 actgcctgaa atgaaagatg tgattgaagc tgcacatgcg ctacgtgaac aaggtattgc
     3301 gcatgttgtt atttcactgg gtgccgaagg cgcgctttgg gttaatgcct ccggcgaatg
     3361 gatcgctaaa ccaccgtcag tcgatgtcgt aagcaccgtt ggcgcagggg attctatggt
     3421 tggtggcctg atttatggct tgctgatgcg tgaatccagt gaacacacac tgcgtctggc
     3481 gacagctgtt gcagccctgg cggtaagtca aagcaatgtg ggtattaccg atcgtccgca
     3541 gttggccgca atgatggcgc gcgtcgactt acaacctttt aactgacagc aggagaggca
     3601 taatgaaaac gctgctgatt attgacgcta atctcggtca ggcacgcgcc tatatggcga
     3661 agaccctgct gggcgcggcg gcgcgaaaag caaaactgga aatcatcgac aatccgaacg
     3721 acgcagaaat ggcgattgtt ctcggtgatt ccatcccgaa tgacagcgcg ctgaacggta
     3781 aaaatgtctg gctgggtgat atttcccggg cagttgcgca ccctgagctg ttcctgagtg
     3841 aagccaaagg ccatgcgaaa ccttacactg cgccggtcac tgcgacagca ccggttgccg
     3901 ccagcggtcc gaaacgcgta gttgcggtga ctgcttgccc gactggcgta gcacacacct
     3961 ttatggcggc tgaagccatt gaaaccgaag cgaaaaaacg tggctggtgg gtgaaagttg
     4021 aaacccgtgg ttctgttggc gcgggtaatg caatcactcc cgaggaagta gccgcagcgg
     4081 atctggtgat tgtggcggca gatatcgaag tggatctggc gaaatttgct ggtaaaccga
     4141 tgtatcgtac ctctaccggt ctggcgctga agaaaacctc gcaggaactg gataaagcgg
     4201 ttgctgaagc aacgccgtat gaaccggcgg gcaaagctca aacggcgacc actgaaggta
     4261 agaaagagag tgcaggcgct tatcgtcact tgctaacggg cgtctcttac atgctgccga
     4321 tggtcgttgc tggtggtctg tgtatcgcgc tttcttttgc ttttggtatc gaagcgttta
     4381 aagagccggg tacgttggca gcggcgctga tgcagattgg tggtggttca gcctttgcgc
     4441 tgatggtgcc ggtactggca ggttatattg ccttctccat tgccgatcgt ccgggcctca
     4501 cgccgggtct gattggcggt atgctggcgg tcagcaccgg ttctggcttc attggcggta
     4561 ttattgcggg cttcctggct ggttacattg cgaagttaat cagtacgcaa ctgaaactgc
     4621 cgcagagtat ggaggcgctg aaaccgatcc tgatcattcc gctaatttcc agtctggtgg
     4681 tcggtctggc gatgatctac ctgatcggta aaccagttgc tggcattctc gaagggctga
     4741 ctcactggct gcagaccatg gggactgcga atgcggttct gctgggggcg atcctcggtg
     4801 gcatgatgtg tactgacatg ggcggtccgg taaacaaagc agcgtacgca ttcggtgtgg
     4861 gtctgctgag tactcaaacc tatggcccga tggcggcgat tatggcggca ggtatggtgc
     4921 caccgctggc aatgggcctg gcaacaatgg tggcgcgtcg caaattcgac aaagcgcagc
     4981 aggaaggtgg caaagccgct ctggtattgg gactgtgctt catttcggaa ggtgcaattc
     5041 cgtttgctgc tcgtgatccg atgcgtgtgc tgccgtgctg tatcgtgggt ggggcgctga
     5101 ctggcgcaat ctcaatggcg attggtgcga aactgatggc accgcacggt ggtctgtttg
     5161 ttctgctgat ccctggcgct attacgccgg tattgggtta cctggtagca attattgccg
     5221 gtacgctggt ggcgggtttg gcctatgcct tcctgaaacg tccggaagtg gacgcagtag
     5281 cgaaagcagc gtaataaaag gtgtgttaca gggcagaaat ttcctgccct gtaacatttg
     5341 atgagcgcaa agagaatgac cagcttgtga atgtaatttt atgaatttaa atggttttat
     5401 ctttcagtag aaacttcaaa cgttcatcaa acactaaggt tatttcctca agggcagatt
     5461 attttctgcc cttttttagt gttcctgttc tgtgcatccc gtcacaaatt cctcgctttt
     5521 gtaattcagg catttaatcg cattttgtga gcctgctcaa atctgcccat tttgcctctc
     5581 tggcggtaat ttactttacg taaaacatgc atactatgag cacatgttta aatatgaaca
     5641 catgtttaaa ccgtctttgc agtatctata ttcttgtgga ttgacagggt tggtaatttt
     5701 ttaccagagg acgtgctatg cgcgaaaagg attatgtcgt aattataggt tcggcgaata
     5761 ttgatgtcgc cggatattca catgaatcat taaattatgc ggattcaaat ccaggtaaaa
     5821 taaaatttac gcctggtgga gtagggcgca atattgcaca aaacctggcg ttgctgggta
     5881 acaaagcctg gctactgagc gccgtaggca gtgattttta tggtcaatcg ctgctaacgc
     5941 aaaccaatca atctggtgtt tatgtcgata aatgcctgat tgtgccggga gaaaatacgt
     6001 cgagttattt atcattactc gataataccg gtgaaatgct ggttgctata aatgacatga
     6061 atattagcaa cgctattaca gctgaatatc tcgcacagca ccgtgaattt attcagaggg
     6121 caaaggtcat tgtcgcggac tgtaatatca gtgaagaggc actggcatgg attctggata
     6181 atgccgccaa cgtacccgta tttgtcgatc cggtttcagc gtggaaatgt gtcaaagtgc
     6241 gcgaccgtct aaatcagaac cacactctga agccaaaccg ccttgaagcg gaaaccctga
     6301 gtgggattgc gctgtcaggg cgtgacgatg tggcaaaagt tgctgcctgg ttccatcaac
     6361 atggcctgaa ccgactggta ttgagcatgg gcggcgacgg cgtttattac agcgatatca
     6421 gcggtgaaaa tggctggtct gcgccgatca aaaccaatgt tattaatgtt accggagcgg
     6481 gcgatgccat gatggcggga cttgcttcgt gttgggtaga cggaatgccg tttgccgaat
     6541 ctgttcgttt cgcacaggga tgttcgtcaa tggcgctctc ctgtgaatac accaataacc
     6601 ccgatttatc gattgccaac gttatatcgt tagtggagaa cgcagaatgt ctgaattaaa
     6661 aatttcccct gaattattac aaatttctcc ggaagtgcag gacgctttaa aaaacaaaaa
     6721 accggttgtg gcgctggaat cgaccattat ttctcacggg atgccgttcc cacaaaatgc
     6781 ccagaccgca attgaagtag aagaaactat tcgtaaacag ggcgcagtac ctgccactat
     6841 cgccattatt ggcggcgtga tgaaagtggg tttaagcaaa gaaaaaattg aattactggg
     6901 tcgtgaaggg cataacgtga ccaaagttag tcgtcgcgat ttaccttttg ttgttgccgc
     6961 cggaaaaaat ggcgcaacca ctgtggcttc aacgatgatt attgcggcgc ttgccggaat
     7021 taaagtattt gccaccgggg gaattggtgg tgtgcatcgc ggggcggaac ataccttcga
     7081 tatttctgcc gatttgcaag aactggcaaa tactaatgtc accgttgttt gtgccggggc
     7141 gaaatctatt ctcgatttag gattaaccac tgagtattta gaaaccttcg gtgtgccgtt
     7201 aattggctat cagactaaag cgctgcctgc gtttttctgc cgtaccagct cgtttgacgt
     7261 cagcattcgt ctcgacagcg ccagtgaaat tgcccgtgca atggcggtga aatggcaaag
     7321 cgggctgaac ggtggcctcg tggtagcgaa cccgatcccg gaacagtttg cgatgccgga
     7381 ggaatctatc aatgcagcca tagatcaagc cgtcgccgaa gccgaagagc agggcgttat
     7441 tggtaaagaa agtacaccgt tcctgctggc gcgcgttgct gagctgacca gcggtgacag
     7501 cctgaaatcc aatatccagc tggtgttcaa caacgccatt ctggcgagtg aaattgccaa
     7561 agaataccag cgtctcgcgg gttaatttct gacgggcaga aagccgtcgc ccgtactcat
     7621 caagcatacc aaaacctggc gctgacgccg ggtttactgg catgaaggga aaatcattat
     7681 ggatataatg agaagtgttg tggggatggt ggtgttactg gcaatagcat ttctgttgtc
     7741 agtgaataaa aagagcatca gtttgcgcac ggttggagcc gcactgctgc tgcaaatcgc
     7801 tattggtggc atcatgctct acttcccacc gggaaaatgg gcagtagaac aggcggcatt
     7861 aggcgttcat aaagtgatgt cttacagtga tgccggtagc gccttcattt ttggttcgct
     7921 ggttgggccg aaaatggatg tcctgtttga cggtgcgggt tttatcttcg cctttcgcgt
     7981 acttccggcg attattttcg ttactgcgct catcagtctg ctgtactaca ttggcgtgat
     8041 ggggctgctg attcgcatcc ttggcagcat tttccagaaa gccctcaaca tcagcaaaat
     8101 cgaatctttt gttgcggtta ccactatttt cctcgggcaa aatgagatcc cggcgatcgt
     8161 taaaccgttt atcgatcgcg tgaatcgcaa cgagttgttt accgcaattt gtagcgggat
     8221 ggcgtccatt gctggttcgg tgatgattgg ttatgccgga atgggcgtac caattgacta
     8281 cctgttagcg gcatcgctga tggcgatccc cggcggtatt ttgtttgcac gtattcttag
     8341 cccggcaacc gagccttcgc aggtcacatt tgaaaatctg tcgttcagcg aaacgccgcc
     8401 aaaaagcttt atcgaagcgg cggcgagcgg tgcgatgacc gggctgaaaa tcgccgctgg
     8461 tgtagcgacg gtggtaatgg cgtttgtcgc aattattgcg ctgatcaacg gcattatcgg
     8521 cggaattggc ggctggtttg gtttcgccaa tgcctctctg gaaagtattt ttggctatgt
     8581 gctggcaccg ctggcgtgga tcatgggtgt ggactggagt gatgccaacc ttgctggtag
     8641 cctgattggg cagaaactgg cgattaacga attcgtcgct tacctgagtt tctccccata
     8701 cctgcaaacg ggcggcacgc tggaagtgaa aaccgtggcg attatatcct ttgcgctttg
     8761 tggttttgct aactttggtt ctatcggtgt tgtcgttggc gcattttcgg ctatttcgcc
     8821 aaaacgtgct ccagaaatcg cccagcttgg tttacgggcg ctggcagcag caacgctttc
     8881 caacctgatg agtgcgacta ttgccgggtt ctttattggt ctggcgtaat gctcgatttg
     8941 tagaccggat aaaacgtatt aagcgttgca tccggcaatc gcgctattgc aaataatttt
     9001 tactgcatca tcccggagaa tttattctcc gggtccatct ccagcgccag tcctgacaac
     9061 tgctttctgt ttttaattag atatcctttc ttttaattaa taaaccgtcg tgaatgaatt
     9121 gcgcgagaac atataaaaga tgtcgataag aaacgcctaa atactctgcc gcttgcgtat
     9181 gcttttcgtg ataaagatca ccttcttgtg agagtaatat aaatgctgcc aggcgattca
     9241 ctaacggaaa tgattgattc tgagttaaag aaacaatatt acgataattt ttatgactta
     9301 aggtgacgca aagttttcgt aaaaataggg tgtcgtttaa taacagcgga cggtaatgtt
     9361 tcatagggag cgcaaggcac caacactctt caatagcctg caccgcacgc ggttcatggt
     9421 ctttgtcgat taactcaatc tcgccaataa aacagggagc ggcaaagaaa tcgatcagcg
     9481 acacgcgacc attggcaagc gtggcgtaaa gcctggcgcg gcctcgcgtc agataaaaca
     9541 gccatgacgg ctgttgccct tcctgcacaa tgtagtcacg cgctaaaaaa tgaaacagcc
     9601 gcgtatcggc tgaaacatcc gttgaaaagc aatccttaaa cgcagattca ctcatcaatt
     9661 gctgcttaag gtcattattg tggatttctt tcacgatgtt tatccgctat gagatttctc
     9721 atatttagca tacgtacgct gttgttaaga ttcaatcatt ggcccggtcg aattttcttt
     9781 gctgtagaaa aaaataacgc aactggaaac agaggaaata aacaatggaa aagagaaaaa
     9841 ttattctgga ttgtgagccg ggtcatgatg atgctattgc aatgatgatg gcagcgaaac
     9901 atccggcaat agatttatta ggcatcacta ttgtagcggg taatcagacg cttgataaaa
     9961 cattaattaa tggcctgaat gtttgccaga aactggagat taatgttccg gtttatgcgg
    10021 ggatgccgca acccattatg cgtaaacaaa tcgttgccga taatattcac ggtgaaaccg
    10081 gactggatgg cccggtattc gagccgctga cccgccaggc agaaagtacc catgcggtga
    10141 aatatatcat cgataccctg atggcaagcg atggcgatat cactctggtg ccggttggtc
    10201 cgctttcaaa tatcgcggtg gcaatgcgta tgcaacccgc aatcctgccc aaaatccgtg
    10261 aaattgtgct aatgggcggc gcttacggta caggcaactt caccccatct gccgagttca
    10321 atatctttgc cgacccggaa gccgcccgcg tggtgttcac ctccggcgtt ccattagtga
    10381 tgatgggcct cgatcttacc aaccagaccg tttgcacccc ggatgtaatt gctcggatgg
    10441 aaagggtagg cggccctgcc ggagagctgt tcagcgacat catgaacttc actctcaaaa
    10501 cgcagttcga atactacggc ctggctggcg gcccggtgca tgacgccacc tgcattggtt
    10561 atctgatcaa ccctgatggc attaaaaccc aggatatgta cgtcgaagtg gacgtcaaca
    10621 gcggcccttg ctacgggcgt acggtctgcg acgaactggg cgttcttggc aagcccgcta
    10681 ataccaaagt cggcatcact attgataccg actggttctg gggcttagtc gaagagtgcg
    10741 tgcgcggcta catcaaaacc cattaagtgt tgataacaag ccgggacagt atcgcaagat
    10801 gcaggcccgg ctatttctga tgggcagaaa gccgtcgccc aaaatgacat gaagagataa
    10861 tcactatgga tgtcatgaga agtgttctgg gaatggtggt actgctggca attgcgtttt
    10921 tactgtcagt aaacaagaag aagatcagcc tgcgtaccgt tggcgcggcg ttagtgttac
    10981 aggtcgtgat tggcgggatt atgctttggt taccgccagg gcgttgggtc gcagaaaaag
    11041 tcgcttttgg cgtccacaaa gtgatggcgt acagcgacgc aggtagcgca tttatcttcg
    11101 gttctctggt cggaccgaaa atggatacct tatttgatgg cgcaggattt atctttggtt
    11161 tcagggtatt accggcaatt atcttcgtca ctgcactggt gagtattctc tactacatcg
    11221 gtgtgatggg gatcttaatt cgcattctcg gcggtatctt ccagaaagct ttaaatatca
    11281 gcaagatcga gtcattcgtc gcggtcacca ccattttcct cgggcaaaac gaaattccgg
    11341 cgatcgtcaa accctttatc gatcgtctga atcgcaatga attatttaca gcgatttgta
    11401 gtggcatggc ctcgattgct ggttcgacaa tgattggtta cgccgcactg ggcgtacctg
    11461 tggaatattt gctggcggca tcgttaatgg cgatccctgg cgggatcttg tttgcccgcc
    11521 tgttaagccc ggctacggaa tcttcgcagg tttcttttaa taacctctct ttcaccgaaa
    11581 caccgccaaa aagcattatt gaagccgatg cgacaggggc aatgaccggg ctgaaaatcg
    11641 ccgccggtgt agcgacagtt gttatggcat ttgtcgccat cattgcgtta attaacggta
    11701 ttatcggcgg cgttggcggc tggtttggtt ttgaacatgc ctcgctggag tccattttag
    11761 gttacctgct ggccccactt gcgtgggtaa tgggtgttga ctggagtgat gcaaatcttg
    11821 ccgggagttt gattggacag aaactggcaa taaatgaatt tgtcgcttat ctcaatttct
    11881 caccctatct gcaaacggct ggcactctgg atgctaaaac cgtggcgatt atttccttcg
    11941 cgttgtgcgg tttcgctaac tttggttcta tcggggtggt ggtgggggcg ttttctgcgg
    12001 ttgcgccaca ccgtgcgccg gaaatcgccc agcttggttt acgcgcgctg gcggcggcga
    12061 cactttctaa tctgatgagt gccaccattg ccgggttctt tattggttta gcgtagtgtg
    12121 caatttatga gccggattag atgcggtcaa gcgtcgaatc cggcaacatt gttacaccat
    12181 tggcactaat gacagcgcgt tatcggcaga cagggtaggg ttgtttaagc tgccgctggc
    12241 gcgtgaaatt gccgcgcagg ccatcgcaaa acgggcgctg tcacggaaat tgtttccctc
    12301 cagaaagcta tataccagac ccgccataaa accatcgtca gcaccaaaac tgtctaccgt
    12361 cgtatgcgct ggcacagtca gcaaaaattg ttcaccatcc ttttcgctgc aataaactga
    12421 ctcatcgggc aaataaacaa acagttgctg aacaccttgc tgatgcaatg cattcactgc
    12481 ggcattacgg tcagcatcgc tggtgatcgc ctgtccccat aaaatttcca gctccggtaa
    12541 agtgggtttc agggtgtgaa tatgcgccag ccagtgtttg attttgcccg ctttgaattc
    12601 tgaaacggta tcgacaaaca ccgggatctc atcagcgagg gtaaagaccc attccagcgc
    12661 ctcggctgtc aggttgcaat cagccagtac cacgcccgca tgacgaagta aatcgcgcga
    12721 cccgttcagt agttgcggtg tcaactgttc cagcagatgg gtatcgttaa ttgccagcac
    12781 ggtttgatcg tctcgattgg caattgccag atacgtcgat gtgctttgac catgcaaacg
    12841 aacgcagccg gagacattca cgccggcacg gcgcgtttct tccaggagca tttcgccata
    12901 aaagtcatcg ccaatcactg aaagcaaatg gacgtcacgg cctaacagcg ccagattgtg
    12961 ggcgatattg cgtccaacgc cgcctgccga acaatgaatt gtaccgggat gagaagccgc
    13021 ttgcgggtaa tggatatccg ccatcccgcg aatatccata ttgattgtcc ccaccactac
    13081 gcagtattcc tgctcggtga gaatgtaacc tttgtcttta atccggcctt tgcgcattaa
    13141 atccataata tgcgccgcaa cgcgcgaacg actgatttgc agcatgtccg caatttcgtt
    13201 ctgctgaatc agcgggttac gccgtaaaat tgcaaggatc tccttttccc ggttatttat
    13261 cttcaggcta ccgctttttc agtttgttgc gctttcagcc aggcgatctc ttctgcccag
    13321 atatccgggt tgatggtttc aaggatcagc ggaatgccgt cgaaacggtc gttctgcata
    13381 atccagcgga acgcatcatg accgatattg ccttcaccga ggctatgatg gcggtcaacg
    13441 cggctgccaa aggtactttt cgcatcgtta aggtgcatcc cgcgcaaata cttaaagccg
    13501 acaatacggg caaaatcagc gaacgttttt tcgcattcgg ctggagtacg caaatcatac
    13561 ccggcagcga aagcatggca ggtatcaatg cagacgccga cgcgggattt atcttccacg
    13621 ccgtcgataa tcgccgcgag atgttcgaat ttaaacccta agttactgcc ctgaccggcg
    13681 gtgttttcaa tcaccgctgt tacaccttga gttttatcca gcgcaatgtt gatggattcg
    13741 gcaatacgcg caaggcaatc ctcttctgaa atctgcatca gatggctgcc agggtggaag
    13801 ttgagcaaag aaagccccag ttgttcgcaa cgctgcattt catctataaa ggcatcgcgc
    13861 gatttttcca gagcttcagt gaccggatgt ccgaggttga tcagataact gtcgtgggga
    13921 agaatttgcg ccgatgtgta gtgatatttt tcacaggcgg ctttgaattc atcgatggtt
    13981 tgcgtcgtga gcggtgcggc acgccactga cgttggtttt tggtgaacaa ggcaaacgcg
    14041 gttgcgtcga tttcggcggc gcgaattgcg gcatttgcca gaccgccagc agcactaacg
    14101 tgcgctccaa tgtatttcat gcgaggactc ctgttaaacc cgctggagga aaacggtaat
    14161 gatagcgggt taacaggagc aagaagtagt ggtttatgcg atgacgcttt gaatcacata
    14221 gttaatcgca ccaccaccaa caatcagcca ggcaaacagt accagtgcca tcagcagagg
    14281 tttcgcccca gcttttttca gcgcgctgac gtgagtggtc agacccagcg ccgccatcgc
    14341 cattgccagc aggaaggtat ccagcgttac cagcatgttc accacgctct gcggtaacag
    14401 gtggaacgag ttaaagatgg caactacgat gaacaagatg gcaaaccacg gaatagtgat
    14461 tttgcttttc tcgccgctgt tcgccccaga cagctgttta acacgcgccg ccagcaggat
    14521 gaggaacgga gccagcatca tcacgcgcag cattttggaa ataactgctg cgttttctgc
    14581 atccgggctg atggcatgac ctgccgccac cacctgcgcc acttcgtgca cagtagaacc
    14641 aatgtagata ccgaaagttt ccggactaaa ccattgagac atcagcggat atatcgccgg
    14701 gtagaggaaa atcgcgacgg tcccgaagat aacaacggtt gcaacagcca cggttacttt
    14761 actggcttcc gctttcacta ccggctcagt cgccagtacc gcggcagcac cacagatact
    14821 gctaccggca ccgatcaacc aactggtgtg cttatccaga ccaaacactt tctgccccag
    14881 gaagcaagcc agcaggaagg tactggacag cgtcagcacg tcaatgatga tcccactgat
    14941 accgacatcg gcaatttgcg agaacgtcag acggaagcca taaagaatga tacccagacg
    15001 taataaatat tgcttggcaa acagcacacc accgtcacag cttttccaga tgtgcggata
    15061 gatggtgttg cctaaaacca tccccaacaa gattgcgagg gtgagggcac taaacccggc
    15121 acccgcaacc gcgggaatgg aaccacccca cagggcgacc ccggtgataa ctgcactcag
    15181 ggctaacccc ggaataaaat gccacagtgt acgatgttgt ttctgtaagg tgatattcgt
    15241 cataaccctc tcctttaacc gggcataagg ttacgactaa ctggtttaaa aataaaattg
    15301 attatatatt tataattaat ctttataagt ggtaagcgac tatgcacatc accctccggc
    15361 agttggaagt ttttgcagaa gtattgaaaa gtggatcaac cacccaggcg tcggtgatgc
    15421 tggcgttgtc gcaatcagca gtgagcgcag ccttgactga cctggaaggg cagcttggcg
    15481 ttcaactgtt tgatcgcgtg gggaaaagac tggttgttaa tgaacacggg cggctgctct
    15541 atccgcgagc gttggcactg cttgaacagg cggttgaaat cgaacaactg tttcgcgaag
    15601 acaacggcgc gattcgtatc tatgccagta gtaccatcgg taactacatt ctgcctgcag
    15661 ttatcgcccg ttatcgccat gattatccgc agttgccgat tgaacttagc gttgggaaca
    15721 gccaggacgt gatgcaagcg gtgctggatt tccgcgttga tattggcttt attgaaggac
    15781 cgtgccacag cactgaaatc atttctgaac cgtggctgga agacgagctg gtggttttcg
    15841 ccgcgccgac ttcgccgttg gctcgtggtc cggtcacctt agaacagctg gccgctgcgc
    15901 cgtggatcct gcgtgaacgc ggttccggca cgcgggatat tgtcgattat ctgttgctgt
    15961 cacatttacc gaagtttgag atggcgatgg aattaggtaa ctccgaggca atcaaacatg
    16021 cggtgcgtca tgggttggga attagttgcc tgtcgcgacg tgtgattgaa gatcaattgc
    16081 aggcaggcac attaagtgaa gttgcggtcc ctctgccgcg cctgatgcgt acgttgtggc
    16141 gtatacatca tcggcaaaaa cacctttcca acgcgctacg gcgctttctg gactattgcg
    16201 atcccgcaaa tgtgccgcgt taagttgctg tacaagaaca tgctggtgct gtgtcgattt
    16261 cgtgacgcag cgccttcagc atgcattcgc cagaaaagag attggctgct ttacttataa
    16321 tccctgggcg atcatgaagg tgtcttataa ccgtgtattt ctgccggaag gattgccaat
    16381 cgtctgctac aatcgcgcca tcatttttaa gatggatagc atttttgtat gggttccgaa
    16441 actaaaacta cagaagcgcc gggcttacgc cgtgaattaa aggcgcgtca cctgacgatg
    16501 attgccattg gcggttccat cggtacaggt ctttttgttg cctctggcgc aacgatttct
    16561 caggcaggtc cgggcggggc attgctctcg tatatgctga ttggcctgat ggtttacttc
    16621 ctgatgacca gtctcggtga attggctgca tatatgccgg tttccggttc gtttgccact
    16681 tacggtcaga actatgttga agaaggcttt ggcttcgcgc tgggctggaa ctactggtac
    16741 aactgggcgg tgactattgc cgttgacctg gttgcagcac agctggtcat gagctggtgg
    16801 ttcccggata caccgggctg gatctggagt gcgttgttcc tcggcgttat cttcctgttg
    16861 aactacatct ctgttcgcgg ctttggtgaa gcggaatact ggttctcact gatcaaagtc
    16921 acgacagtta ttgtctttat catcgttggt gtgctgatga ttatcggtat cttcaaaggc
    16981 gcgcagcctg cgggctggag caactggaca atcggcgaag cgccgtttgc tggtggtttt
    17041 gcggcgatga tcggcgtagc tatgattgtc ggcttctctt tccagggaac cgagctgatc
    17101 ggtattgctg caggcgagtc cgaagatccg gcgaaaaaca ttccacgcgc ggtacgtcag
    17161 gtattctggc gaatcctgtt gttctatgtg ttcgcgatcc tgattatcag cctgattatt
    17221 ccgtataccg atccgagcct gctgcgtaac gatgttaaag acatcagcgt cagtccgttc
    17281 accctggtgt tccagcacgc gggtctgctc tctgcggcgg cggtgatgaa cgcagttatt
    17341 ctgacggcgg tgctgtcagc aggtaactcc ggtatgtatg cgtctactcg tatgctgtac
    17401 accctggcgt gtgacggtaa agcgccgcgc attttcgcta aattgtcgcg tggtggcgtg
    17461 ccgcgtaatg cgctgtatgc gacgacggtg attgccggtt tgtgcttcct gacctccatg
    17521 tttggcaacc agacggtata cctgtggctg ctgaacacct ccgggatgac gggttttatc
    17581 gcctggctgg ggattgccat tagccactat cgcttccgtc gcggttacgt attgcaggga
    17641 cacgacatta acgatctgcc gtaccgttca ggtttcttcc cactggggcc gatcttcgca
    17701 ttcattctgt gtctgattat cactttgggc cagaactacg aagcgttcct gaaagatacc
    17761 attgactggg gcggcgtagc ggcaacgtat attggtatcc cgctgttcct gattatttgg
    17821 ttcggctaca agctgattaa aggaactcac ttcgtacgct acagcgaaat gaagttcccg
    17881 cagaacgata agaaataagt ttcctccctt ccttgctaag ccctctcaac cgagagggct
    17941 ttttcaattc catttccctg acaaatcatg cggatataaa atttaacatt tggattgata
    18001 attgttatcg tttgcattat cgttacgacg caatcaaaaa aggctgacaa atcagaggct
    18061 gttccggctt tctgggatga tcacctgcat acaaataagt ccaccgcgat gctgccgtac
    18121 gcaaggggac gtaaagaaga tgtgagcgat acccatttta ttttcgtagt tacctcatgg
    18181 agatatggaa tgtttaggtt gaaccctttc gtacgggtcg ggctgtgttt gtccgctatt
    18241 tcttgtgcat ggcctgtgtt agcggtcgat gatgatggcg aaacgatggt tgtcactgca
    18301 tcttccgtgg aacaaaatct taaagatgca cccgccagta tcagcgtcat tacccaggaa
    18361 gacctgcagc gaaaaccggt acagaatctg aaggatgtcc tcaaagaagt gcctggcgta
    18421 caactgacga acgaagggga taaccgtaag ggcgttagta ttcgtggtct ggacagcagc
    18481 tacaccctga ttcttgtcga cggtaaacgc gttaactccc gcaatgccgt cttccgccac
    18541 aatgatttcg atctgaactg gatcccggtc gattccatcg aacgtattga agtggttcgt
    18601 ggcccgatgt cgtcgctata cggctccgat gcgctcggcg gtgtagtgaa tatcatcacc
    18661 aaaaaaatcg gtcagaaatg gtcgggtacc gttaccgtcg ataccaccat tcaggaacat
    18721 cgcgatcgcg gtgataccta taacggtcag ttctttacca gtggaccatt aattgatggt
    18781 gtgctgggaa tgaaagctta cggcagcctg gcaaaacgtg aaaaggatga cccgcaaaac
    18841 tcaacgacca ccgataccgg agaaacgccg cgtattgaag gattctccag ccgcgacggc
    18901 aatgtcgaat ttgcctagac accgaatcaa aatcacgatt ttactgccgg atacggtttc
    18961 gaccgtcagg atcgtgattc cgattcgctg gacaaaaacc gcctggaacg ccagaactac
    19021 tgcgtcagcc ataatgggcg ttgggattac ggcaccagcg aactgaaatc ctacggtgag
    19081 aaagtcgaga acaaaaaccc tggcaacagc agcccgataa cttccgaaag caatacggtc
    19141 gacggcaaat acacgttgcc gctgacggcg attaatcagt ttctcacggt tggcggtgaa
    19201 tggcgtcacg acaaacttag cgatgcggtg aacctgaccg ggggaaccag ctccaaaacg
    19261 tctgccagcc agtacgcgct gtttgtggaa gatgaatggc ggatcttcga gccgctggcg
    19321 ctgacgaccg gcgtgcgtat ggacgatcac gaaacctacg gtgaacactg gagtccgcgt
    19381 gcctacatgg tttataacgc caccgacacc gtaacggtga aagggggctg ggcgacggca
    19441 tttaaagcac cttctctgtt gcaacttagc cctgactgga cgagcaattc ctgccgtggc
    19501 gcatgtaaga ttgtgggtag cccggatctg aaaccagaaa ccagcgaaag ttgggagctg
    19561 gggctttact acatgggtga agaaggctgg ctggaagggg gtgaatccag cgttatcgtt
    19621 ttccgtaacg atgtgaaaga tcgtatcagc atcagccgta cgtctgacgt caacgctgca
    19681 ccgggctacc aaaactttgt tggttttgag acgggcgcta acggacggcg cataccggta
    19741 tttagctact acagcgttaa caaagctcgt attcagggcg tggaaaccga actgaaaatt
    19801 ccgttcaacg atgaatggaa actgtcgatc aactacacct acaacgatgg tcgtgatgtc
    19861 agcaacggcg aaaacaaacc gctatccgat ctgccgttcc atactgctaa cggtacgctg
    19921 gactggaaac cgctggcgct ggaagactgg tcattctatg tttctggtca ctataccggg
    19981 cagaaacgcg ccgacagcgc gacggctaaa acaccgggcg gttataccat ctggaatacc
    20041 ggcgcggcct ggcaggtgac taaagacgtc aaactgcgcg caggcgtgct gaaccttggc
    20101 gacaaggatc tcagtcgtga cgactacagc tataacgaag acggacgtcg ttactttatg
    20161 gcagtggatt atcgcttctg atgagaagat gcccggcgaa ccgggcggac tttcacttca
    20221 gtaaatactg cgcatggaag cgcaggtgat cctctataaa agaggcgatg aagtagtaac
    20281 tgtgatcata tcccggctga atacgcagcg tcatcggcca cgctttctgg cgcgcggctt
    20341 ctgccaatac cgcaggttgc aactggtcgg caagaaactg atcattatcg ccctgatcga
    20401 taagcgtcgg gatcgcatcc tgcgcgttac tggcatacat cagcgcgcaa ctatcccatt
    20461 ccagcatgca tttttgtcct cacctaaata ggtgctaaac gctttgattc cccacgggac
    20521 gctgcacgga ttcacaatgg gcgcaaaggc cgaaacgctg gtgtatttac ccggattttt
    20581 cagcgccata atcagcgcac cgtgaccacc cattgagtga ccgctaatgg cgcagcggtc
    20641 gctgacatta aattgcgact gaaccagcgc cggtaattca tcgcgcagat aatcatacat
    20701 ccggtaatgc gtcgcccacg gcggttgcgt ggcattaaga taaaagcctg cgccctggcc
    20761 taaatcgtag ccatcgtcgt tggcaacctt ttcgccgcgc gggctggtgt ctggcatcac
    20821 cagtacaatc cccagttccg ccgctacccg ctgggcaccc gccttggtgg tgaagttctc
    20881 gtcattgcag gttaatccgg aaagccagta cagcactggt ggcggggtgt gatcacgagg
    20941 gggggggaga aagatactga acgtcatcgg gcagtttaag gtactggagt cgtgtcgcca
    21001 gcgttgctgc cagccttcaa aacagcggtg ctcttcgagc atttccatgc gtggctcctt
    21061 gttgtgttgt ttgcaatttc ttcatcatac cgtttattca atgtgctgtg agtaactttc
    21121 acttccgtat ttgcataacg atgttttaac atctgctgat gaaaggcagc ggcaattaca
    21181 ataattatcg ctgtgaatac tggattatgt gcgccgcctc acgcacaata atcaagctgt
    21241 aaatcagctt aataactttg cccccacgca gggtggaggc gtcacacctg caggagaaat
    21301 cataaatgcc atcactcagt aaagaagcgg ccctggttca tgaagcgtta gttgcgagag
    21361 gactggaaac accgctgcgc ccgcccgtgc atgaaatgga taacgaaacg cgcaaaagcc
    21421 ttattgctgg tcatatgacc gaaatcatgc agctgctgaa tctcgacctg gctgatgaca
    21481 gtttgatgga aacgccgcat cgcatcgcta aaatgtatgt cgatgaaatt ttctccggtc
    21541 tggattacgc caatttcccg aaaatcaccc tcattgaaaa caaaatgaag gtcgatgaaa
    21601 tggtcaccgt gcgcgatatc actctgacca gtacctgtga acaccatttt gttaccatcg
    21661 atggcaaagc gacggtggct tatatcccga aagattcggt gatcggtctg tcaaaaatta
    21721 accgtatcgt gcagttcttt gcccagcgtc cgcaggtgca ggaacgtctg acgcagcaaa
    21781 ttcttattgc gctacaaacg ctgctgggca ccaataacgt ggctgtctcg atcgacgcgg
    21841 tgcattactg cgtgaaggcg cgtggcatcc gcgatgcaac cagtgccacg acaacgacct
    21901 ctcttggtgg attgttcaaa tccagtcaga atacgcgcca cgagtttctg cgcgctgtgc
    21961 gccatcacaa ctgattaaaa ggcaggaacc atggagcgca acgtcacgct cgattttgtt
    22021 cgcggcgtcg ccattctggg gatcctgcta ttaaacatca gcgcctttgg gctaccaaag
    22081 gcggcttatc tcaatcccgc ctggtacgat gctattacgc cgcaggatgc atggacctgg
    22141 gcatttctcg atctcatcgg ccaggtgaaa ttcctcacgc tttttgcgct gctgtttggt
    22201 gctggcctgc aaatgttgct gccccgtggc agacgctgga tccagtcgcg gttaacgctg
    22261 ttagtcttgt tgggctttat tcacggttta ttgttctggg acggcgatat tctgctggct
    22321 tacgggctgg tggggcttgg cgttttgctg ttattggggc tgatttccga cagccagacc
    22381 agccgcgcct ggacgccgga tgcatcggct attttgtatg aaaaatactg gaagcttcac
    22441 ggcggcgttg aagcgatcag taatcgtgcc gatggtgttg gcaacagttt actggcactg
    22501 ggcgcacagt atggctggca actggcaggg atgatgctca ttggtgcggc gctgatgcgc
    22561 agcggctggc tgaaagggca gttcagctta cgtcactatc gtcgtactgg ttttgtgctg
    22621 gtggcgattg gggtgaccat taaccttcct gccatcgccc tgcaatggca gctggactgg
    22681 gcgtatcgct ggtgtgcctt cttacttcaa atgccgcggg aactgagtgc gccgtttcag
    22741 gcgattggct atgcgtcgct gttttatggc ttctggccgc aattgagccg ctttaagctg
    22801 gtgcttgcga tcgcctgcgt cggacggatg gcgctgacca actatctatt gcaaacgctg
    22861 atttgtacca cgctttttta ccacctcggt ttgtttatgc attttgaccg cctggagctg
    22921 ctggcgtttg ttattccggt atggctggcg aatatcctct tctctgttat ctggctgcgt
    22981 tacttccgcc aggggccggt ggaatggctc tggcgtcagt taactttgcg tgctgccgga
    23041 ccggcaatat ctaaaacatc aagataacga tctggattac attcgttaac aaaacggctg
    23101 taaccgtttc cattgctgtg actcgattca cgaagtcctg tattcagtgc tgacaaaata
    23161 gccgccagca agcagtcatt tactgcaatc tcataacagg tagtgaatat gatcaccatt
    23221 cgtgatgtag cgcgtcaggc tggcgtctct gtggcaacgg tttcccgggt gctcaataac
    23281 agcacgctgg tcagtgccga cacgcgtgaa gcagtaatga aagccgtgag tgagctggat
    23341 tatcggccaa acgccaatgc ccaggcgctg gcaactcagg ttagcgacac cattggcgtg
    23401 gtggtgatgg acgtttctga tgcgtttttc ggcgcgctgg taaaagcggt ggatctggtc
    23461 gcccagcagc atcagaaata cgtgctaatc ggcaatagct atcatgaagc ggaaaaagag
    23521 cgtcacgcca ttgaggtgtt aattcgccag cgttgtaatg cgttgattgt tcactcaaaa
    23581 gcattgagtg acgatgaact ggcgcaattt atggataaca ttcccggtat ggtgttaatc
    23641 aaccgcgttg tgccggggta cgcccatcgt tgcgtttgcc tggataatct cagcggtgcc
    23701 cgaatggcga cgcgcatgtt gctgaataac ggtcatcaac gtattggtta tctttcttcc
    23761 agccacggca ttgaagatga cgccatgcgt aaagcaggct ggatgagtgc gttgaaagag
    23821 caggatatta ttccgccgga aagctggatt ggcactggta cgccggacat gccgggcggt
    23881 gaggcggcga tggttgaact gctggggcgc aatctacaac ttaccgctgt atttgcttat
    23941 aacgacaata tggccgctgg cgcactgaca gcattaaaag ataatggcat tgcgattccg
    24001 ttacatctct caatcatcgg tttcgatgat attcccatcg cccgttacac cgacccgcaa
    24061 ttaacgaccg tgcgttatcc cattgcttca atggctaaat tagccaccga actggccttg
    24121 cagggggcag caggcaatat tgatcctcgt gccagccact gttttatgcc gacgttagtg
    24181 cgtcgccatt ctgtcgcaac gcgccagaat gcggcggcga tcactaactc aacaaatcag
    24241 gcgatgtaac cgctttcaat ctgtgagtga tttcacagta tcttaacaat gtgatagcta
    24301 tgattgcacc gttttaatgt tgtaacccgt atgtaacagt gaataatcac tttcaccgag
    24361 gtaatagcgt cataacaaca attaaagccg ttttctggag cgttaccggg catggaagaa
    24421 cgaattttaa aaagtgagct tcggcgttca gtaacacttc attaactcta ctgccccgcc
    24481 gagcatttat ctcaagcact accctgcata agaaaaaccg gagataccat gaataagaag
    24541 gtgttaaccc tgtctgctgt gatggccagc atgttattcg gtgccgctgc acacgctgct
    24601 gatactcgca ttggtgtaac aatctataag tacgacgata actttatgtc tgtagtgcgc
    24661 aaggctattg agcaagatgc gaaagccgcg ccagatgttc agctgctgat gaatgattct
    24721 cagaatgacc agtccaagca gaacgatcag atcgacgtat tgctggcgaa aggggtgaag
    24781 gcactggcaa tcaacctggt tgacccggca gctgcgggta cggtgattga gaaagcgcgt
    24841 gggcaaaacg tgccggtggt tttcttcaac aaagaaccgt ctcgtaaggc gctggatagc
    24901 tacgacaaag cctactacgt tggcactgac tccaaagagt ccggcattat tcagggcgat
    24961 ttgattgcta aacactgggc ggcgaatcag ggttgggatc tgaacaaaga cggtcagatt
    25021 cagttcgtac tgctgaaagg tgaaccgggc catccggatg cagaagcacg taccacttac
    25081 gtgattaaag aattgaacga taaaggcatc aaaactgaac agttacagtt agataccgca
    25141 atgtgggaca ccgctcaggc gaaagataag atggacgcct ggctgtctgg cccgaacgcc
    25201 aacaaaatcg aagtggttat cgccaacaac gatgcgatgg caatgggcgc ggtagaagca
    25261 ctgaaagcac acaacaagtc cagcattccg gtgtttggcg tcgatgcgct gccagaagcg
    25321 ctggcgctgg tgaaatccgg tgcactggcg ggcacagtac tgaacgatgc taacaaccag
    25381 gcgaaagcga cctttgatct ggcgaaaaac ctggccgatg gcaaaggtgc ggctgatggc
    25441 accaactgga aaatcgacaa caaagtggtc cgcgtacctt atgttggcgt agataaagac
    25501 aacttggctg aattcagcaa gaaataagac tgattgggcg cagtcgttta ctgcgccctt
    25561 attgtatggc caacaaggta aaattatatg gtcagctcaa cgactccgtc ttccggggaa
    25621 tacttgttgg aaatgagcgg tatcaacaag tcctttcctg gtgttaaggc acttgataac
    25681 gttaatttaa aagtccggcc acattctatc catgcattaa tgggggaaaa cggtgcagga
    25741 aaatcgacat tattaaaatg cctgtttggt atttatcaaa aagactccgg caccatttta
    25801 ttccagggta aagagatcga tttccattcc gccaaagagg cactggaaaa tggtatttcg
    25861 atggtacacc aggagttaaa cctggtatta caacgttcgg tgatggataa catgtggctg
    25921 gggcgatatc ccaccaaagg catgtttgtc gatcaggaca aaatgtaccg cgaaaccaaa
    25981 gcgatttttg atgaactgga tattgatatc gatccgcgtg cgcgcgtcgg cacattatcc
    26041 gtttcgcaaa tgcagatgat cgaaatcgcc aaagcgtttt cctataacgc gaaaattgtg
    26101 attatggatg aaccgacttc ttcgttaacc gaaaaagagg tcaatcatct gttcactatt
    26161 attcgtaaat taaaagagcg cggctgcggt attgtttata tctcgcataa aatggaagaa
    26221 atcttccagt tatgtgatga agttaccgta ttgcgcgacg gtcagtggat cgccaccgaa
    26281 ccgctggcag gactgacgat ggacaagatc atcgccatga tggttgggcg ttctcttaat
    26341 cagcgttttc ctgacaaaga aaacaagccg ggcgaagtca tcctcgaggt gcgtaaccta
    26401 acgtcactgc gccagccgtc gattcgcgat gtctcgtttg atctgcataa aggggagatc
    26461 ctcggtattg ccgggctggt gggggcgaaa cgtaccgata ttgttgagac gttatttggt
    26521 attcgcgaga aatcggctgg caccattacg ttgcacggca aacagatcaa taaccataat
    26581 gccaacgaag ccataaacca cggatttgca ctggtaactg aggagcgccg ctcaacggga
    26641 atttatgcct atctggatat tggttttaac tcgttaattt ccaatattcg caactacaaa
    26701 aataaagttg gtttactgga taactcgcgg atgaaaagcg atacccagtg ggtgattgat
    26761 tcgatgcggg taaaaacgcc gggtcatcgg acgcaaattg gttcgctctc cggtggtaat
    26821 cagcaaaagg tgattattgg tcgctggcta ctaacgcaac cagaaatatt aatgctcgat
    26881 gaaccgacgc gcggtattga tgtcggggcg aagtttgaaa tttatcagtt aattgccgaa
    26941 ctggcgaaga aaggcaaggg gattattatt atctcctctg aaatgcctga gttgttaggg
    27001 ataacagacc gtattctggt catgagcaat ggtctcgttt ccggaattgt cgatacaaaa
    27061 acaacaacgc aaaacgaaat tctgcgtctt gcgtctttgc acctttaaga ttaggggctt
    27121 cccatgagtg cgttaaataa gaaaagtttt cttacttacc tgaaagaggg cggtatttac
    27181 gtcgttcttt tagttttgct ggcgattatt attttccagg acccaacatt tttaagtctg
    27241 ttgaacttaa gtaatattct cacccagtca tcggtgcgta ttattatcgc gctcggtgtg
    27301 gcagggttaa ttgtcaccca ggggaccgat ctttctgctg gtcgtcaggt agggctggcg
    27361 gcagtggtgg ctgcgacatt attgcagtcc atggataacg ccaacaaagt gttcccggaa
    27421 atggcgacga tgccgattgc gctggttatt ctgattgtct gtgccattgg tgcggtgatc
    27481 ggtttgatca acggtctgat tatcgcttat ctcaacgtga cgccgttcat taccacgctc
    27541 ggcacgatga tcatcgtcta tggcatcaac tcgctctatt acgactttgt cggggcgtcg
    27601 ccaatttctg gttttgacag tggcttctct acctttgctc agggctttgt cgcgctgggg
    27661 agtttccgtc tctcttacat caccttctac gcgttgattg cggtggcgtt cgtctgggtg
    27721 ttgtggaaca aaacccgctt cggtaagaac atttttgcca ttggcggtaa cccggaagcg
    27781 gcaaaaatat ctggtgtcaa cgtcggcctg aacctgctga tgatctacgc gttgtctggc
    27841 gtgttctatg cctttggcgg gatgttagaa gccggacgta tcggctctgc caccaacaac
    27901 ctcggcttta tgtatgagct ggatgctatc gcggcgtgcg tggtaggcgg tgtatcgttc
    27961 agcggcggtg tggggacggt gattggcgtg gtgaccgggg taattatttt taccgtcatc
    28021 aactatggcc tgacgtatat cggcgtaaac ccatactggc agtacatcat caaaggggcg
    28081 attattatct tcgccgtagc gctggattca ctgaaatacg cgcgtaagaa gtgatgtgat
    28141 tgcggatatt gccggatgcg tctctggttt tacaacgtta ccgggtgttc tttctcgcct
    28201 ttcttaaact tcacttcccc gagctcaata caacccaccg ggcagacgtg accacaaagc
    28261 agacaaccca cacatttctc ggtattacaa tgcggtgtgc gggttttctc gctccattcc
    28321 atcgcctggt gaccgccgtc gtaacaggaa atataacagc gtccacagcc aacacatttg
    28381 tcaagattga tacggggata gacaatataa ctgcggtcta aatcttccgc cgggacaata
    28441 ttgttattcg ccagacctac catctcctgc aacgaatcaa atccctgatc ggcgagataa
    28501 tgcgataacc cgctcgccat atcttctacg atgcgatagc cgtactgcat aatgccggtg
    28561 gtgacctgta acgttgctgc gccgagcaat aaaaactccg ccgcatcttc ccaggtttca
    28621 atgccgccga taccgctgat tgggaaatcg cgcagttctg gatgggtgcg catttgctga
    28681 atgaagcgca gggcgatcgg ttttaccgct ttaccggaat atccggaaat actcgattta
    28741 ccgttaacga tcggcatacc gattttctga ttaagatcga tattggtgat ggatttaacg
    28801 gtgttaattg ccgcaatgcc atctgcgccg ccgcgcttcg ccgccagcgc cacttcgcac
    28861 atatcaccga tattcggcgt catcttcgcc agcattggca gcgtggaacc ccgtttcact
    28921 gcccgacaat atttttctac cagctccggg ctttgcccga catcgctacc catcgcatga
    28981 gaagtcattt gcggacagga gaagttacac tcgatcatat ccgcgccagc tgctcccatt
    29041 gctgctcatt ttcccccatg atcgaagcga tcaatacttt gtccgggtaa tcttccttca
    29101 gccgacgcag ggcggtcaga ttctcttcca acggatgttc agcaatctgc tccatatttt
    29161 tgaagccgat aaaaccggta tcttctttca ccagatgatc aaaacgcggc gagacttcgt
    29221 tggcgataaa aaagccgatc gttttaaaca ccacaccgcc ccaacctgtg tcgtaggctt
    29281 tggcacacat ctcatagcag ttgcctaccg gcgaagaaga gaggcagaac gggttgggaa
    29341 agttcacgcc gcaaaaagta atcgaaagat ctttcgttaa catgagcaag ctccctctaa
    29401 atagtgatga atcgcctcgg cggcttcttt cccggttttc acggcataga ccacggtttt
    29461 gtcaccctca acaatatcgc cagcagcaaa gacttgcgga tcgcggatct gataattttg
    29521 tgttttgatg gtgttacgct gctgctctaa ctccgcaaag gcatccagtc tggcatgctg
    29581 accgacggcg agaataattt tatctgccgc catcgtcagt tcgcctgata accgtacatg
    29641 cttaaacgtg actttattgc cttccacggc tactggcgtg aatccatcga tgatcgaaac
    29701 gcccagctcc cgggcgctgg caaactcttt ttcgcttgcc ggaaactcat ctaactcttc
    29761 acgtgctacg caagttaccg cctgacagcc gagaactttc agcgtgctgg ctacgtccat
    29821 cgcgacatca ccgccgccga taattaatgc gctttgtgga atgctgatat cgccttgcgc
    29881 ctgtcgtaca cgttgcagga agtcgacggc aatctcaacg tcgctatgct caaacagcgg
    29941 tagcccggaa ccgcttgata accccacggt gaccagtacc gcgcggtttt ccgctttcag
    30001 ttgctccagg gtgagtgttt taccgatttc gttattgcac ttaatggtca cgcccatttt
    30061 ttcaatacgg gcgatctctg catccagcac tgattgcggt aaccggaatt gcggaatacc
    30121 gttacgcaac cagccaccgg ggtgcgcttc tttctcataa atcgtgacgt cataaccctg
    30181 gtttgtcagt gtcacactgg cctgcaatcc ggcaggacct gcgccaataa tcgcgacttt
    30241 gccgagcgtt ttagtaccgg gctgataaat ttccatcccg gtttgttgtt caaaatcagt
    30301 aacaaaacgc tgtaagcggc cgatatcaat gggagcatca acaccggcac gggtacaacc
    30361 gctttggcat aatttctccg tcgggcacac tctggcacaa acggcaccga gggcattatt
    30421 ttcgcgaatt gtctcggcag cgcctttaaa attacgaaag tagattgagc gaataaattt
    30481 ccccggatcg gtctgtgccg ggcaagcctg actacaggga gcgtcgtgac ataataaaca
    30541 gcgagaggct tctttaatcg ccagtaacgg cgtaaaagcc ggagtgagtt catccagata
    30601 attttgttgc ggcatgtgat cttccttaat gctatgagta attatggcga acgctaaata
    30661 aatattgcgt gctgatatta aggctatgcg cgtggggaaa tttaagctgt gaaaggattc
    30721 acaaatagct gcggttcaga atgttatatc cgcagcttat tgagtgaagt aataattatt
    30781 ctcaataatt aacagaaaag agaaaatatt aacgatagaa aataagataa acaatcatgc
    30841 cgatagataa aattccgaag ccaatatatg ccaccatata agcgaagaag agtccgggat
    30901 tttttgctcg ccagacatac accggttccg gtgcattttt aacccgttta tcaatcgcgc
    30961 ctgcgcgcca gcctttccat gcttcgcgga aacaaaatat cgggataagg aaaaaaacgg
    31021 caacgacaaa aaactgctgt acatccataa ctactttcct tgtttctttt gtaattcaag
    31081 taactgttcg ggtgtaacgg cgggataccc ctgcgcatct tccggcacct ggtgcatagc
    31141 cggaataggg accagcggcc ctaaaaaacg cggttcacgt ttaaaaatat aaaggtcagc
    31201 cagcgcaccg aaacgggcgg caaattcgcg aatacgtact gacagcatat ctttcggtga
    31261 cggtacggca taacactgag cctgaatccc catatgcagc gcaataaata atgctcgctc
    31321 acagtggaaa cgttgggtga taataatgaa atcattagta tcgaaaactt tgcgtgtacg
    31381 cacgatggaa tccagcgtac gaaagcctgc gtaatcgaga acaatatctg atgggtcgac
    31441 accagcagcg attaaatctt tgcgcatggt catcggctcg ttataacttt gcaatgcgtt
    31501 atcgccgctc agtaatagat aatttacctt accgctgtta taggcattaa tcgctccttg
    31561 aatgcggtag cgataatact gattaataac gccggtacga taatattttg ctgtgccgag
    31621 caccacgccg acctggcggt aggggagatc ctgcaattcg tcgtagatat aaggcgcggt
    31681 tttccagctc atccagcgat cgaggccgag cacagtcaac agcagcaagc cgatcaggac
    31741 taacaggctg aggaacacgc gctttaacat gcaggagact cgagctaagg aaaatttcca
    31801 tcaggctact ttacccgctg gttttgcgca agaaacggca aactgtttgc cgggaatttc
    31861 gacaccagga gagggctggc aagccggaca aggcgttcac gccgcatccg gcaccaggct
    31921 taagcgagaa gcacgcggtc gatactgtga cagccgagag ctttcagcgt tgcggaggtg
    31981 gcatcccact gaatcaacgg cgcatcggct ttttctgcca gaaccgcgcg ctggatatcc
    32041 gggtaatcat aacccttgag attcaacaga attaacgccc cctgcaacgg cggcagagtc
    32101 gggttgaatg cggcgttttc agcgtagctg ccgctgaaaa tacgaccgtc tttacattcc
    32161 agcgcgacac cgcttggtga cttactgtaa ggcatgtgcg aacggtttgc cgctgcaatc
    32221 gccgcctgag aaagcgcatc accagtcagc gcatagccgt gatcctgctc gtccatcagc
    32281 agcgttttaa tctccagatc tttcggccca aaggcatccg gcagatagtc acgcagcgcg
    32341 tgtgcctcgc ggcccggcaa atgaatacgc agatccagac cgctgttcag ttcattcata
    32401 aactgacggc agtgaccaca aggcgtatag ttaacggtga tggctgcaag cgctttttca
    32461 ccactcaacc aagcgtggct gatcgcgctt tgttcggcat gaacggtttg ctgcattgtt
    32521 gcaccaataa attccatatt ggcaccaaaa taccaggttc cgctcacacc gcgcgcaatt
    32581 gcgccaacat taaaattcga caatggcgta cgcgcacagg ccgccgccag cggaagtagt
    32641 gcgaatgcca gcgcgtcttc gtccagtccc gttgcgctct tcagcgatga gacttgctcc
    32701 ccggtcaaca aagcggggaa gtacttgtct gccagaatag gttccagtgc agattgcaag
    32761 ttatccgcaa gttgggcaaa agcggtttga aaacgtggat gcatggcgtt gcctcataat
    32821 catgtaatgg gataacagtt tacgaacccg ttatggcttt atatgtgatc tgaatctcat
    32881 taattatgca acacgtgaaa cttacatcaa aaatgcgcga cgtatcgcaa attttagccc
    32941 attactgcca gaataatcgg gaaaaggaac ggtgcgatca gcgaagtaat tatcccgcat
    33001 aacaccagcg ctagcgaact aaatgcacct tcctgataat ccagctcggc gcagcgcgcc
    33061 gtaccgaggg cgtgcgaggc agtccccatt gccagaccgc gcgcagcttt ggtacgaata
    33121 cgcatcgcat taagcaatgt atggccaaat accgcgccga ggatgccgac gaaaatcacg
    33181 caaactgcgc tgattgccgg aataccgcca atactgccgc caaccgccat tgcaataggc
    33241 gtggtgactg atttcggcag gattgacgcg gcgatttccg gtgaagcgcc catcaacaat
    33301 gccacggaag tccccgtcac catcgcaacc acgctgccga taaaacagat ggtgatgatc
    33361 gatttccagc gcgcgcggat ctggtgtagc tgctcatata aaggataggc cagcgcgacg
    33421 accgccggtt gcagcagatc gtttagcact tcgctacctt taaagtaact gtcgtaagag
    33481 atgccggtca gcattaaaaa aggaatgatc accaccattg ctaccaacaa cgggttaagc
    33541 aggggaaact tataccgtgc cgccagtttg cgggcggcaa aaaagacaat caacgtcagc
    33601 ggtaatgacc accagatatt cgccatcatt cttctgatcc tttctgacct acgactttac
    33661 gttcaccgtg taccagttgt gaactccagc tcaccaccag aaaaaccacc agcgtactga
    33721 cagcacagga aaccactacc gggccaaact gcgcgcggag caaatcaaaa tattgcatga
    33781 cgcctacgcc aatcggcaca aacaatagcg ccatatagcg aatcagtacg tagcaccccg
    33841 gattgaccca tttcgccgga agaatttgca aggccagcag gacaaacagg atcagcatcc
    33901 cgatgatgct gcccggaatg gttaccggta gcagggaagc aatgaaaatg cctgcataca
    33961 ggcaggcata aatcaggacg aaagcgcgta aatattgcca gataatgttc agtgtcttac
    34021 tcataatcaa caccccagat gagatggatt catcatacaa ttaattcgta aaacgtgcta
    34081 ccgatcacat taagaaatca gcgcttattt tttcgctacc cggtattacg caatgagcag
    34141 cgaactcagg catacatcaa gcatagagag ggattgcagt ttgcagcaat ccctctttgc
    34201 agacctttaa tccgctgacc ataggcgcta acatagcgcc tcattttttt gcgggtgatg
    34261 atatgcgtgt attactggca ccgatggagg gagtgcttga ctctctggtg cgtgaattgc
    34321 tgaccgaagt taacgactac gatctgtgca tcaccgagtt tgtccgcgtg gtggatcaac
    34381 tgctgccggt aaaagtcttt catcgcattt gccctgagct acaaaacgcc agccggacac
    34441 catctggtac gctggtgcgc gtgcagttgt taggtcagtt cccacaatgg ctggcagaga
    34501 acgccgcccg tgcggtggag ttaggtttct ggggcgtgga tctcaattgc ggctgcccgt
    34561 cgaaaacggt taacggtagc ggcggcgggg cgacgttact caaagatcct gaactcatct
    34621 accagggggc aaaagcgatg cgtgaagctg taccggcgca tttgcctgtc agcgtgaaag
    34681 tgcgtctggg ctgggacagc ggtgagaaga aatttgaaat cgccgatgcg gttcaacagg
    34741 ctggcgctac ggagctggtg gtgcatgggc ggacgaaaga gcagggttac cgcgcggagc
    34801 atattgactg gcaggcgatt ggcgagattc gccagcggct gaatattccg gtgattgcca
    34861 acggtgaaat ctgggactgg cagagcgcgc aacaatgcat ggcgatcagc ggctgcgacg
    34921 cagtgatgat tggtcgcggg gcgctcaata ttcccaacct gagccgggtg gtaaaatata
    34981 acgaaccgcg aatgccgtgg ccggaggtgg ttgctttgct gcaaaaatat acccgtctgg
    35041 aaaagcaggg cgataccggg ttatatcacg ttgcgcggat taaacagtgg ttgagttatt
    35101 tgcgtaaaga atacgatgaa gcaacggaat tatttcagca tgttcgggtg ttgaataact
    35161 cccctgatat tgcaagggct attcaggcaa ttgatatcga gaaactctaa aaaccatagt
    35221 gatgtttttg acaatgtgtt agctctttat caataggccg cacggttgtt tggtggcgtc
    35281 aaaatcgccg aggcgttccc tgaaggccgg ggcagcccac atggatgtgg gctgagggcg
    35341 cgttttacag ggatgttacc tcgcgcccga cccggtagcc gtaagggata agccgagggc
    35401 accgcgcagc ggcgattttg ttcgccagag cccggggggg cagggggcgg cggcgattgg
    35461 tcgccccctg cgcgctcctt gcgccagtga ttatatgaag caatgcacat gacaggagcg
    35521 caacaggatg agagactgaa atattctcgt tattttcgga tctgatgaca ttaattaatt
    35581 ttaaaatatc atcttaaata cgccagtcac caccagtagc ccaatcagga aaataatcaa
    35641 tacagcccag agtataattt tcattggcgt tcctttattt gtgagtgctg aataagtata
    35701 ggaaaaaacg aagccctttg ccgttatcca taaacgatta agataaatcg gaagatatca
    35761 tttacttagt ctatattttt ctgggtatgt cgttttattg ctactgatgt attgccacgc
    35821 gaaactaaaa aatatatcgc aacacacggt aatctccgcg caccgtttct tacctgatta
    35881 ttcccccatg aatcgtgatt ccttttatcc agccatcgcc tgttttccgc tgttactgat
    35941 gctggccggg tgtgcgccta tgcatgaaac ccgccaggcg ttaagccagc aaacgcccgc
    36001 tgcacaagtt gacaccgcat tacccacggc gctgaaaaat ggttggccag acagccaatg
    36061 gtggctggag tatcacgata atcagctcac ttcattaatt aacaatgcac tacagaacgc
    36121 accggatatg caggtggctg aacagcgcat ttagctagct gaagcccagg cgaaggcagt
    36181 tgccactcag gatggtccgc agatcgactt ttcggcggat atggagcggc aaaaaatgtc
    36241 ggcagaaggg ctaatggggc cgtttgctct gaacgatccg gccgcaggta cgaccggtcc
    36301 gtggtacacc aacggaactt ttggcttaac ggcgggctgg catctcgata tctggggaaa
    36361 gaatcgggcg gaggttactg cccgcctggg tacggttaaa gcacgggcgg cggaacgcga
    36421 gcaaacccgc caattgctgg ctggcagcgt agcccgcctg tactgggagt ggcaaaccca
    36481 ggcggcgtta aacacggtct tgcagcaaat agaaaaagag cagaacacca ttatcgcgac
    36541 cgatcgccag ctatatcaga acgggattac ttcttcagtt gacggtgtgg aaaccgatat
    36601 taatgccagc aaaacccggc agcagctcaa cgatgtcgcg gggaaaatga aaattattga
    36661 ggcacggtta aatgcactca caaatcacca gactaagtca ttgaagctta aaccggtcgc
    36721 gttgccgaaa gtggcaagcc agcttcctga tgaactgggg tactccttac tggcccggcg
    36781 ggcagatttg caggcggcgc actggtacgt tgagtcatcg ttaagcacta ttgatgcggc
    36841 aaaagcggca ttttatcctg acatcaacct gatggccttc ctgcaacagg atgcgttgca
    36901 cttaagcgat ctgttccgtc attccgcgca gcaaatgggc gttacggcag gcctgacgct
    36961 acctattttc gatagtggtc gtcttaacgc caatctcgat atcgcaaaag ccgaaagcaa
    37021 cttgtctatc gccagctaca acaaagcggt ggttgaagca gtgaatgacg tggcgcgggc
    37081 agccagtcag gttcagacac tggcggagaa aaaccagcat caggcgcaaa ttgagcgcga
    37141 tgccttgcgt gtggtaggtc ttgcgcaggc ccgctttaac gcgggcatca ttgctggttc
    37201 ccgcgtcagc gaagccagaa tcccggcgct gcgtgagcgg gccaatggcc tgttattgca
    37261 agggcagtgg ctggatgcct ccattcgact caccggtgcg ctgggcgggg gttacaaacg
    37321 ctgatgatat gcgccttcta tacttaacgt ttattcagcg ttaagtggag aactcgatgg
    37381 cataggtcgc gataattacc gcttccgatt cggggatcgg caaagagtgc gcgttattac
    37441 tggcgcagca ggggtttgat attggcatta cctggcactc agatgaagaa ggggcaaaag
    37501 ataccgcgcg tgaggtggtt acccacggcg tacgtgcgga gatcgtgcaa ctggatctcg
    37561 gcaatctgcc agaaggtgcg caggcgctgg agacactcat ccagcgattt gggcgtattg
    37621 atgtgctggt gaataatgcg ggtgcaatga ccaaagcgcc gtttcttgat atggcttttg
    37681 atgagtggcg caagattttt accgttgatg ttgatggtgc atttttatgc tcgcaaattg
    37741 cggctcgtca gatggtgaaa taagggcagg gcggtcgcat catcaacatt acgtcggtac
    37801 atgaacatac gccgctgccg gatgccagcg cctacacagc cgctaaacat gcgctcggcg
    37861 ggttaaccaa aacgatggcg ctggagttgg tcaggcataa gattttggtg aacgcagtcg
    37921 cgcctggggc gatagccacg ccaatgaaca gcatggatga tagcgacgtg aagcccgacg
    37981 cggagccttc gattcctcta cggcgttttg gcgcaacgca tgagattgcc agcctggtgg
    38041 tgtggctttg ttcggagggc gcaaattaca cgacagggca gtcgttgata gtggatggcg
    38101 gctttatgtt ggcgaatccg cagttcaacc cagaatagcg cagtgaggtg cgtggatatt
    38161 tcaatgggtt aggccagata agacaccagc gtcgcatcag gcatggaaca cccggtgccg
    38221 gatgtggagt gaatgcttat ctgtggcaac tgctttacgc ctgattatcc ggctttttct
    38281 tcccgcgccg tttcagccac cagcgcacgc ccaccaccag aaccacgacc agaatcaacc
    38341 agacccagtg cttcaaatgc tggtcgagat tgtgcaacca cggcgcaatc acctgaccac
    38401 cagcgtaacc aatagtggta aaaatcaacg cccaggcaaa tgcgccgaga atattcagcg
    38461 gcagaaagat tttcggcggc agctggctgg caccaatcag cgtcgggcca atcacccgaa
    38521 agccatacat aaagcgcgta ccaatgacaa acagatacgg atggcgttgg ataagtttct
    38581 gtgcccgctc aattttatcc tgatgtttcg agaaacggcg taacagcttg ccgccaaacc
    38641 gccgcccgca cagatagagc acctggtcgc caatcatgcc accaagcgcc acagaaagta
    38701 ccaccagtgg gaactttaat agcccctgat gtgccgcaac gcctcccagc aaagtcacgg
    38761 tttcaccttc cgccaggcta ccgataacca gcgcggcata accatattgt gagatgagtg
    38821 tattgagatc cataggccag tttgtctccc tttggttgcc tgtaagcata caccctgtga
    38881 atcattcacg ctgaaaaagg acatacatca gacggtgcaa attacatgga tgaacaaaaa
    38941 ataatcacaa gctggcttat acttgaactg tcaggttcat gaactcacaa caagggggcg
    39001 gtatgagcca tgtctgggga ctgttttccc atcccgatcg tgaaatgcag gtgattaatc
    39061 gcgaaaacga aacgatttct caccattaca cccaccacgt tttgctgatg gcggcgattc
    39121 cggtgatttg cgccttcatt ggcactacac agattggctg gaattttggc gatggcacta
    39181 tcctgaagtt atcctggttt accggactgg cgctggctgt cttgttttac ggcgttatgc
    39241 tggctggggt tgcggtgatg gggcgggtca tctggtggat ggcgcgcaat tacccgcagc
    39301 gtccgtctct tgcgcactgc atggtcttcg cgggctacgt tgcaactccg ctgtttttaa
    39361 gcggtctggt ggcgctttat ccactggtct ggttgtgtgc gctggtcggc acggtggcgc
    39421 tgttttacac cggctacctg ctttaccttg gtattccgag cttcctgaat atcaacaaag
    39481 aggaaggcct tagcttctcc agttcgacgc tggcgattgg cgtgctggta ctggaagtgc
    39541 tgctcaccct aaccgttata ctatggggct acggttatcg tttgttctag aaagttcttt
    39601 gacgttgcat tgctggcgta aatgtcagca atgcagcatt tactcacgat tctcctttga
    39661 cgatcgcact ttttgctcgt tatgatggcg tcgctagcct cagtaaatcc ttactccggc
    39721 ggtgcgcaac ccgtgcgcgt gaaccactat ctgaatgctc atcatgccga aatttcgagt
    39781 ttctttattt agcttggccc tgatgctggc tgtgcctttt gcaccgcagg ccgttgcgaa
    39841 aacggcagcc gctaccaccg cttcacaacc ggaaattgcc tccggtagcg cgatgattgt
    39901 tgatctgaat accaacaaag tgatctattc gaaccacccg gatctggtgc gtccgattgc
    39961 gtctatcagc aaattaatga ccgcgatggt tgtgctggat gcacgactgc cgctggatga
    40021 aaaactaaaa gtggatatca gccagacgcc ggagatgaaa ggggtctatt cgcgcgtacg
    40081 actgaatagc gaaatcagcc gtaaagatat gctgttgctg gcgctgatgt cttcagaaaa
    40141 ccgcgcggcg gcaagccttg cgcaccatta tcccggtggt tacaaagcct ttattaaggc
    40201 aatgaatgcg aaagcgaaat cgctcggaat gaacaacacg cgctttgttg aacctaccgg
    40261 attgtcggtg cataacgttt caactgcccg tgacttaacc aaactgctca ttgccagcaa
    40321 acaatatccg ttgatcgggc agttaagtac tacccgggaa gatatggcaa ccttctctaa
    40381 cccgacgtat acgctgccgt tccgcaatac taatcatctg gtgtatcgcg ataactggaa
    40441 tattcagtta accaaaaccg gctttaccaa tgcggcgggc cattgtctgg tgatgcgtac
    40501 ggttatcaat aataaaccgg tggcgctggt agtgatggac gcgtttggca aatataccca
    40561 ttttgccgat gccagccgcc tgcgtacctg gattgaaacc ggtaaagtga tgcctgtgcc
    40621 ggcagcagcg ttgagctata aaaaacaaaa agccgcccaa atggcggcgg cggggcagac
    40681 ggcacagaac gattaagtga attcggatgg cgatactctg ccatccgtaa tttttactcc
    40741 acttcctgcc agtttttccg tttactggtt ttaccgatcc ccggattcat gctattggtc
    40801 ggatcattct cgcgatagaa cttctgcaac gtctccggtg ctttatacaa atgaccgacg
    40861 ttatgctcgg cagggtactg cgcgccgcgc tgctgtagca gttccagcat ctgttctttt
    40921 aacgcatgca catccacgcc ttttttcact atgtaatcct gatggaagac atagcacata
    40981 aaatggccgt aatagagctt gtgcaccagc tggctgtcga tctccggcgg taaatgctca
    41041 taccactcgg tgtcgttacg ccgcagagcg atatccagtg ccagaatgtc ttcgacttca
    41101 tcggaatgca ccgcctgata acgaattgct gcgcccgcag cggcgaaacg gtgtaaaaac
    41161 gctttgctac cttcctccgg cgtacagaca aagaaatcgc cctcggcctg tttgaaataa
    41221 tccaccagcc acgatttggc ttcaccgacg ccatcgcctg ccatttttaa cagcagatga
    41281 tgctcgtatt tatcgcgcca gtttttcatg cgcggcggta aatggctggg gaacaggtga
    41341 ccgaattttt gcattgcacg gtcagtaaaa tgcggacgga agaatttcac tttctccagc
    41401 atcgcatcgg tgcgtccctt gagattaaag aagaacggca tcttgtcggt gcctaactta
    41461 tcaatcatca ggaaggtgtc tttgccgtat ttttccgcaa tatcgtagat atcccggtgc
    41521 atatattccc cggcaaccgg cagattttcg aagttagcca gaatatgacg gcggatttcg
    41581 gtcagcactt ccggctggtt ggtgccgata taaaacacct gctgattttt ttccgcttcg
    41641 aaggtatcaa gacgtaccgc aaagacggcc agcttcccgg cgcaaccaga agattcaaat
    41701 aaccgatccg gatcggcgtt ataacgtgcg ggcgtgtcgg cttcaatatc acgaacgcgg
    41761 tggacatagt cataatcgtg ggcgtgacga ccatcgtgac gcacatcgtc atctttgatg
    41821 cgatcgtcat ccagcttgct aaggatctgc tccggcgttt cgcccagatc aatccccaga
    41881 tggttcacca gcgtcagttt gccatcttca tctatacgcg cgaataacga catttcggta
    41941 tacgccgggc cacgttgcac cagcgagccg ccggagttgt tacaaatacc gccgatgacc
    42001 gatgcgccta tacacgatga tccaatcacc gaatgcggtt cgcgtcccag cggtttgagg
    42061 gctttttcca gcgaatagag cgtggtgccc ggataggcca gcacctgttc gcccttgccg
    42121 agaacgtgca gcttgtcgag acgcagggtg ctgatgatca cgatatcgcg atcatagtcg
    42181 ttaccgtttg gcgtcgatcc ttcggtcagg cctgtattgg cagcctgcat cagaataatt
    42241 ttgtcggcgg tgacgcaggc tttcagcacc cgccacaatt ccagtagtga gccagggaaa
    42301 acgacagcca gcgcgtcgcc ctgaccagaa cggaagccct tgcgatagcg ggccgttttt
    42361 gcgggatcgg tgagcaggtg tgaatgaccg accagacggg caagttcatt caaaaaggct
    42421 ttattatcag ttgttgtcat ggaagacatt ttccactcct tgtggtggcg aaaaaatatc
    42481 acaaactaga atagcgcctt gtatgacaat tcagcgagtg aatattttga aaattcagac
    42541 aataaactga gcattctttt tctctctcca tcatgcttat ggcaaactac ggtttttgca
    42601 ccattttcgc tgccacgtcg ggcaacaaag gaagaaaaat ccatatgaaa tggctatgtt
    42661 cagtaggaat cgcggtgagt ctggtcctgc agccagcact ggcggatgat ttattcggta
    42721 accatccatt aacgcctgaa gcgcgggatg cgttcgtcac cgatctgctc aagaaaatga
    42781 cggttgatga gaaaattggt cagctgcgct taatcagcgt aggcccggat aatccgaaag
    42841 aggcgatccg cgagatgatc aaagacggtc aggttggggc gattttcaac accgtaaccc
    42901 gtcaggatat ccgcgccatg caggatcagg tgatggaatt aagccgcctg aaaattcctc
    42961 ttttctttgc ttacgacgtg ctgcacggtc agcgcacggt gttcccgatt agcctcggtc
    43021 tggcctcgtc ttttaacctc gatgcggtga aaacggtcgg gcgtgtctct gcttatgaag
    43081 cggcagatga tggcctgaat atgacctggg caccgatggt cgatgtctcg cgcgatccgc
    43141 gctggggacg tgcttccgaa ggttttggcg aagatacgta tctcacctca ataatgggta
    43201 aaaccatggt ggaagcgatg cagggtaaaa gcccggcaga tcgttactcg gtgatgacca
    43261 gcgtcaaaca ctttgccgca tacggcgcgg tagaaggcgg taaagagtac aacaccgtcg
    43321 atatgagtcc gcagcgcctg tttaatgatt atatgccgcc gtacaaagcg gggctggacg
    43381 caggcagcgg cgcggtgatg gtggcgctga actcgctgaa cggcacgcca gccacctccg
    43441 attcctggct gctgaaagat gttctgcgcg accagtgggg ctttaaaggc atcaccgttt
    43501 ccgatcacgg tgcaatcaaa gagctgatta aacatggcac ggcggcagac ccggaagatg
    43561 cggtgcgcgt ggcgctgaaa tccggaatca acatgagtat gagcgacgag tactactcga
    43621 agtatctgcc tgggttgatt aaatccggca aagtgaccat ggcagagctg gacgatgctg
    43681 cccgccatgt actgaacgtt aaatatgata tggggttgtt taacgaccca tacagccatt
    43741 tggggccgaa agagtctgac ccggtggata ccaatgccga aagccgcctg caccgtaaag
    43801 aagcgcgtga agtggcgcgc gaaagcctgg tgttgctgaa aaaccgtctc gaaacgttac
    43861 cgctgaaaaa atcggccacc attgcggtgg ttgggccact ggcggacagt aaacgtgacg
    43921 tgatgggcag ctggtccgca gccggtgttg ccgatcaatc cgtgaccgta ctgaccggga
    43981 ttaaaaattc cgtcggtgaa aacggtaaag tgctgtatgc caaaggggcg aacgttacca
    44041 gtgacaaagg cattatcgat ttcctgaatc agtatgaaga agcggtcaaa gtcgatccgc
    44101 gttcgccgca agagatgatt gatgaagcgg tgcagacggc gaaacaatct gatgtggtgg
    44161 tggctgtagt cggtgaagca caggggatgg cgcacgaagc ctccagccgg accgatatca
    44221 ctattccgca aggccaacgt gacttgattg cggcgctgaa agccaccggt aaaccgctgg
    44281 tgctggtgct gatgaacggg cgtccgctgg cgctggtgaa agaagatcag caggctgatg
    44341 cgattctgga aacctggttt gcggggactg aaggcggtaa tgcaattgcc gatgtattgt
    44401 ttggcgatta caacccgtcc ggcaagctgc caatttcctt cccgcgttct gtcgggcaga
    44461 tcccggtgta ctacagccat ctgaacaccg gtcgtccgta taatgccgac aagccgaaca
    44521 aatatacttc gcgttatttt gatgaagcta acggagcgct ttatccgttc ggctatggtc
    44581 tgagctatac cactttcacc gtctctgatg tgaaactttc tgcgccgacc atgaagcgtg
    44641 acggcaaagt gacggccagc gtgcaggtga cgaacaccgg taagcgcgag ggggccacgg
    44701 tagtgcagat gtacttgcag gatgtgacgg cttccatgag tcgccctgtg aaacagctga
    44761 aaggctttga gaaaatcacc ctgaaaccgg gcgaaactca gactgtcagc ttcccgatcg
    44821 atattgaggc gctgaagttc tggaatcaac agatgaaata tgacgccgag cctggcaagt
    44881 tcaatgtatt tatcggcact gattccgcac gcgttaagaa aggcgagttt gagttgctgt
    44941 aatgctttag taagggcgcg acgtttgcgc cctttgcctg atgttcatat cttaacttgc
    45001 tgaattttcg ccctttgtag gccggataag gcgctcgcgc cgcatccggc aggaaacgcc
    45061 ggaccgaata tgcctcttaa gtatttatct gattctgcaa ctgaatcctt ccgctcaggc
    45121 taactctgtc attatcaact atgcttttct cttaattcgc tgaaaacaac agtaaaagaa
    45181 tgaggaaagc atcatgccac tcttaaagct ctgggcaggt tcactggtta tgttggcagc
    45241 cgtgagcctg ccgctacagg cggcttcccc cgttaaagtc ggttcaaaaa tcgataccga
    45301 aggcgcgctg ctcggcaaca tcatcttgca ggtactcgaa agccacggcg taccgacggt
    45361 gaataaagtg caactcggaa cgactcctgt ggggcgcggg gcgattactt ccgatgaact
    45421 ggatatctat ccggaatata ccggcaatgg cgctttcttc tttaaagatg aaaacgatgc
    45481 agcgtggaaa aatgccgggc agggctacga gaaagtcaaa aagctcgatg cggagcacaa
    45541 caagttaatc tggctgacgc ccgcgcctgc aaataacacc tggactatcg ccgtgcgtca
    45601 ggatgtggca gagaaaaaca aactcacttc gcttgccgac ctgagtcgtt atctgcaaga
    45661 gggcggcacc ttcaaactgg cagcctcggc agagtttatc gaacgcgccg atgcgttacc
    45721 cacgtttgaa aaagcctatg gctttaagct cggtcaggat cagttgctgt cactggctgg
    45781 tggcgacacg gcggtgacga ttaaagccgc tgcccagcaa acctctggcg ttaatgccgc
    45841 aatggcttac ggcactgacg gtccggtcgc ggcgctgggg ctgcaaacct taagcgatcc
    45901 gcaaggcgtg caacctatct acgcgcctgc accagtggtg cgtgaatcgg tgttgaagga
    45961 gtatccgcaa atggcacagt ggctacagcc agtcttcgcc agcctcgatg caaaaacatt
    46021 gcagcaactg aatgccagca ttgcagtgga aggactggat gccaaaaaag tggctgccga
    46081 ctacctgaaa caaaaagggt gggcgaagta atttcccgtg acttatctac gtattaatcc
    46141 tgttctggcg ctgctgttgt tgctgacggc aatcgcagcg gcgctgccgt ttatcagtta
    46201 cgcgcctaat cgtttagtct cgggtgaagg gcttcatctc tggcaactgt ggccgcaaac
    46261 gatctggatg ctggtgggcg ttggttgcgc ctggctgaca gcctgttttg tccccgctaa
    46321 aaaaggcagc atttttgcac tcattctggc gcaattcgtc ttcgtattgt tggtgtgggg
    46381 agctggaaag gcggcgaccc aactggcgca aaatggcagt gcgctggcgc gtaccagcct
    46441 cggcagaggt ttctggctgg ctgcggcgct ggcattgctg gcctgtagcg atgccatccg
    46501 ccgaatctcc acgcatccgc tgtggcgctg gttgttgcat atgcagattg ccattattcc
    46561 gctgtggttg ctgtactccg gcacgcttaa cgatctctca ctaatgaaag aatacgccaa
    46621 ccgtcaggat gtgtttgacg acgcgctggc acaacatctg acgttgctgt ttggtgcggt
    46681 gctgcctgcg ttagtgattg gtgtgccgtt gggcatctgg tgctactttt ccactgcgcg
    46741 gcagggggca attttttctc tgctcaatgt cattcagacc gtgccttcgg tggcgctctt
    46801 tggcctgttg attgcgccgc ttgccgcgct ggttacggcc tttccgtggc tggggaagct
    46861 cggcatagca ggaaccggaa tgacacccgc actgattgcg ctggtgctct atgccttgct
    46921 gccgctggtg cgaggcgtgg tagtcggttt gaaccagatc ccgcgcgatg tgctggagag
    46981 cgccagagcg atggggatga gcggggcgca gcgtttcctg catgttcagt taccgctggc
    47041 gttaccggta tttctgcgca gcctgcgggt ggtgatggtg caaactgtag gtatggcggt
    47101 gattgcggcg ttaatcggcg caggcggttt tggtgcgctg gttttccagg ggctgctaag
    47161 cagcgccatt gatttagtgt tgctgggggt gatcccggta attgttctgg cggtgcttac
    47221 cgacgcgctg ttcgatttgc ttatcgcact gctgaaggtg aaacgtaatg attgaattta
    47281 gccatgtcag caaactgttc ggcgcacaaa aagccgttaa cgatctcaat ctcaattttc
    47341 aggaagggag tttttcggtg ctgattggca catctggctc cggcaaatcc accaccctga
    47401 aaatgattaa ccgcctggtg gagcatgaca gcggagtgat ccgctttgcc ggagaagaaa
    47461 ttcgctcgct gccagtactg gagttgcgcc gccggatggg ctatgccatt caatctattg
    47521 gcctgttccc ccactggagc gtggcgcaaa acatcgctac cgtgccgcaa ttacaaaaat
    47581 ggtcgcgggc gcggattgac gatcgtatcg acgaattaat ggcgctactg gggctggagt
    47641 caaatttgcg tgagcgttat ccgcatcagc tttccggtgg tcagcagcaa cgtgtgggag
    47701 tggcgcgtgc actggctgcc gatccgcaag tcttactgat ggatgaacct tttggcgcac
    47761 tggacccggt aacgcgcggc gcgttgcaac aagagatgac gcgcattcac cgtttgctgg
    47821 ggcgcactat tgtgctggtc actcatgata ttgatgaggc gctacggctg gcagaacatc
    47881 tggtattgat ggatcacggt gaagtggtgc agcaggggaa tccgctgacg atgctgactc
    47941 gtccggcgaa tgattttgtc cgccagtttt ttggacgtag tgaactgggg gtgcacctgc
    48001 tttcgttacg tagcgtggcg gattacgtgc gtcgcgaaga acgggcagaa ggtgaggcac
    48061 tggcagaaga gatgacgcta cgcgatgcgc tctccctgtt tgtcgcgcgg ggatgcgagg
    48121 tgctgccggt ggtgaacacg cagggccagc cttgcggcac gctgcatttt caggatctgc
    48181 tggtggaggc gtaagcgtat gaagatgttg cgcgatccgc tgttctggct cattgctctt
    48241 tttgtggcgc tgattttctg gctgccttac agccagccgc tgtttgctgc cttgttccca
    48301 caactgccac gacccgttta tcagcaagaa agttttgcag ctctggcact ggctcatttc
    48361 tggctggtgg gaatttcgag tttgtttgcg gtgatcattg gcacaggtgc cggaattgct
    48421 gttactcgcc cgtggggcgc ggaatttcgc ccactggtgg aaactattgc cgccgttgga
    48481 cagacttttc cgcctgtcgc agtgctggcg attgccgttc cggtgatcgg ctttggtctg
    48541 caaccagcga ttatcgcctt gatcctttac ggtgtgctgc ccatcctgca ggtgacactt
    48601 gccgggctgg gagcgattga tcccagcgtg acagaagttg cgaaaggtat gggaatgagt
    48661 cgaggtcagc gactgcgtaa ggtcgagcta ccgctggcgg ctccggtgat tctggcgggc
    48721 gtgcgaactt cggtgattat caacattggt acggcgacga tcgcctcaac ggtaggggcc
    48781 agtacgctgg gtacgcccat catcatcggg cttagcggat ttaataccgc gtatgtgatc
    48841 cagggggcgt tactggtggc actggcggcg atcatcgcag accgcctgtt tgaaaggctg
    48901 gtgcaggcgc ttagccagca cgcaaaataa aggtataacc tgcgagcatg acgccaccaa
    48961 ttccgcctaa cgccataaac aggaacaggg cgatgacccc aattttagct atgcgcataa
    49021 tgcactcctt atgttaacga aaggattgta cagtaaagcg catttgttaa cgaatcatta
    49081 aataccgagt gggaaaatat catggccttg ttcttgccaa ctggtgagtt gctgctgttg
    49141 ggcggaggtt cgattttcac cgcaccacac cagcaatgta cggccttcga atagttcagg
    49201 gcgtagttga ttgagcgagt gggcgagcga ggacatcaat gcgccatcct tgttgactgg
    49261 caatccagcc ctccagccac agacgggtgg tatcctgaat attccagcca accaccagcg
    49321 catctttacc ctgtttttta cgtgccgaag ccagacaaat ggcgatgtag ttgatcagta
    49381 cgccgtcgag gatcgccagc agcgcctgga gagtcggttg ttggcactga agccgtcggc
    49441 gcagaggaat aaacagatgt gtggtgagtg tctgggcggg gtaatcctga ccgcgctctt
    49501 tgatccacgt tcgcaggcta tgtagattgc c
//
LOCUS       DAJTQQ010000022        48110 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000022 DAJTQQ010000000
VERSION     DAJTQQ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.022"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            19..312
                     /locus_tag="OO309_001374"
     CDS             19..312
                     /locus_tag="OO309_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5846566.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(353..1543)
                     /gene="nepI"
                     /locus_tag="OO309_001375"
     CDS             complement(353..1543)
                     /gene="nepI"
                     /locus_tag="OO309_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR5846567.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1754..2206)
                     /locus_tag="OO309_001376"
     CDS             complement(1754..2206)
                     /locus_tag="OO309_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR5846568.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2259..3593)
                     /gene="adeQ"
                     /locus_tag="OO309_001377"
                     /gene_synonym="yicO"
     CDS             complement(2259..3593)
                     /gene="adeQ"
                     /locus_tag="OO309_001377"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR5846569.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3768..5534
                     /gene="adeD"
                     /locus_tag="OO309_001378"
     CDS             3768..5534
                     /gene="adeD"
                     /locus_tag="OO309_001378"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR5846570.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5580..6971)
                     /gene="uhpT"
                     /locus_tag="OO309_001379"
     CDS             complement(5580..6971)
                     /gene="uhpT"
                     /locus_tag="OO309_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR5846571.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7109..8428)
                     /gene="uhpC"
                     /locus_tag="OO309_001380"
     CDS             complement(7109..8428)
                     /gene="uhpC"
                     /locus_tag="OO309_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR5846572.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8438..9940)
                     /gene="uhpB"
                     /locus_tag="OO309_001381"
     CDS             complement(8438..9940)
                     /gene="uhpB"
                     /locus_tag="OO309_001381"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR5846573.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(9940..10530)
                     /gene="uhpA"
                     /locus_tag="OO309_001382"
     CDS             complement(9940..10530)
                     /gene="uhpA"
                     /locus_tag="OO309_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR5846574.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10605..10895)
                     /gene="ilvN"
                     /locus_tag="OO309_001383"
     CDS             complement(10605..10895)
                     /gene="ilvN"
                     /locus_tag="OO309_001383"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5846575.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(10899..12587)
                     /gene="ilvB"
                     /locus_tag="OO309_001384"
     CDS             complement(10899..12587)
                     /gene="ilvB"
                     /locus_tag="OO309_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR5846576.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12693..12791)
                     /gene="ivbL"
                     /locus_tag="OO309_001385"
     CDS             complement(12693..12791)
                     /gene="ivbL"
                     /locus_tag="OO309_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR5846577.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13356..13445
                     /gene="tisB"
                     /locus_tag="OO309_001386"
     CDS             13356..13445
                     /gene="tisB"
                     /locus_tag="OO309_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR5846578.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13725..14909
                     /gene="emrD"
                     /locus_tag="OO309_001387"
     CDS             13725..14909
                     /gene="emrD"
                     /locus_tag="OO309_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR5846579.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(14917..15414)
                     /locus_tag="OO309_001388"
     CDS             complement(14917..15414)
                     /locus_tag="OO309_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR5846580.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15411..15773)
                     /locus_tag="OO309_001389"
     CDS             complement(15411..15773)
                     /locus_tag="OO309_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR5846581.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15763..16110)
                     /locus_tag="OO309_001390"
     CDS             complement(15763..16110)
                     /locus_tag="OO309_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR5846582.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            16219..16632
                     /locus_tag="OO309_001391"
     CDS             16219..16632
                     /locus_tag="OO309_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846583.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16679..18121)
                     /locus_tag="OO309_001392"
                     /pseudo
     CDS             complement(16679..18121)
                     /locus_tag="OO309_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18156..19871)
                     /locus_tag="OO309_001393"
     CDS             complement(18156..19871)
                     /locus_tag="OO309_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCR5846584.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20038..20931
                     /locus_tag="OO309_001394"
     CDS             20038..20931
                     /locus_tag="OO309_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5846585.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(20928..21742)
                     /locus_tag="OO309_001395"
                     /pseudo
     CDS             complement(20928..21742)
                     /locus_tag="OO309_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21742..23358)
                     /locus_tag="OO309_001396"
     CDS             complement(21742..23358)
                     /locus_tag="OO309_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR5846586.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23653..24327
                     /locus_tag="OO309_001397"
     CDS             23653..24327
                     /locus_tag="OO309_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5846587.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24366..26027)
                     /locus_tag="OO309_001398"
     CDS             complement(24366..26027)
                     /locus_tag="OO309_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR5846588.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26223..26651)
                     /gene="ibpB"
                     /locus_tag="OO309_001399"
     CDS             complement(26223..26651)
                     /gene="ibpB"
                     /locus_tag="OO309_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR5846589.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26763..27176)
                     /gene="ibpA"
                     /locus_tag="OO309_001400"
     CDS             complement(26763..27176)
                     /gene="ibpA"
                     /locus_tag="OO309_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR5846590.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27170..27242)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27481..27813
                     /locus_tag="OO309_001401"
     CDS             27481..27813
                     /locus_tag="OO309_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR5846591.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27815..29047)
                     /locus_tag="OO309_001402"
     CDS             complement(27815..29047)
                     /locus_tag="OO309_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR5846592.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTWTPDSQWLVFDVRPSGASFT
                     GETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHR
                     RGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPA
                     LDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVG
                     NHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQR
                     RLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTH
                     NKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDG
                     QWLAWMEGGQLWITETDR"
     gene            29148..30248
                     /gene="cbrA"
                     /locus_tag="OO309_001403"
     CDS             29148..30248
                     /gene="cbrA"
                     /locus_tag="OO309_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR5846593.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30209..31546)
                     /locus_tag="OO309_001404"
     CDS             complement(30209..31546)
                     /locus_tag="OO309_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5846594.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31621..32769)
                     /gene="dgoD"
                     /locus_tag="OO309_001405"
                     /pseudo
     CDS             complement(31621..32769)
                     /gene="dgoD"
                     /locus_tag="OO309_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32766..33383)
                     /gene="dgoA"
                     /locus_tag="OO309_001406"
     CDS             complement(32766..33383)
                     /gene="dgoA"
                     /locus_tag="OO309_001406"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR5846595.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33367..34245)
                     /gene="dgoK"
                     /locus_tag="OO309_001407"
     CDS             complement(33367..34245)
                     /gene="dgoK"
                     /locus_tag="OO309_001407"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR5846596.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34242..34931)
                     /gene="dgoR"
                     /locus_tag="OO309_001408"
     CDS             complement(34242..34931)
                     /gene="dgoR"
                     /locus_tag="OO309_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR5846597.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLAAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35209..35865
                     /locus_tag="OO309_001409"
     CDS             35209..35865
                     /locus_tag="OO309_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846598.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(35911..36723)
                     /gene="yidA"
                     /locus_tag="OO309_001410"
     CDS             complement(35911..36723)
                     /gene="yidA"
                     /locus_tag="OO309_001410"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR5846599.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(36837..37235)
                     /locus_tag="OO309_001411"
     CDS             complement(36837..37235)
                     /locus_tag="OO309_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR5846600.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37475..39889)
                     /gene="gyrB"
                     /locus_tag="OO309_001412"
     CDS             complement(37475..39889)
                     /gene="gyrB"
                     /locus_tag="OO309_001412"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR5846601.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(39918..40991)
                     /gene="recF"
                     /locus_tag="OO309_001413"
     CDS             complement(39918..40991)
                     /gene="recF"
                     /locus_tag="OO309_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR5846602.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(40991..42091)
                     /gene="dnaN"
                     /locus_tag="OO309_001414"
     CDS             complement(40991..42091)
                     /gene="dnaN"
                     /locus_tag="OO309_001414"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR5846603.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42096..43499)
                     /gene="dnaA"
                     /locus_tag="OO309_001415"
     CDS             complement(42096..43499)
                     /gene="dnaA"
                     /locus_tag="OO309_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR5846604.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43796..43876
                     /locus_tag="OO309_001416"
     CDS             43796..43876
                     /locus_tag="OO309_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846605.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44106..44246
                     /gene="rpmH"
                     /locus_tag="OO309_001417"
     CDS             44106..44246
                     /gene="rpmH"
                     /locus_tag="OO309_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR5846606.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44263..44622
                     /gene="rnpA"
                     /locus_tag="OO309_001418"
     CDS             44263..44622
                     /gene="rnpA"
                     /locus_tag="OO309_001418"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR5846607.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44586..44843
                     /gene="yidD"
                     /locus_tag="OO309_001419"
     CDS             44586..44843
                     /gene="yidD"
                     /locus_tag="OO309_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR5846608.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            44846..46492
                     /gene="yidC"
                     /locus_tag="OO309_001420"
     CDS             44846..46492
                     /gene="yidC"
                     /locus_tag="OO309_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR5846609.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46598..47962
                     /gene="mnmE"
                     /locus_tag="OO309_001421"
     CDS             46598..47962
                     /gene="mnmE"
                     /locus_tag="OO309_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR5846610.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11901 a        13027 c        12177 g        11005 t
ORIGIN      
        1 ttgtaaaagg ttgaatccat gaaaccaacg acactactgc ttattttcac ttttttcgct
       61 atgccaggca tcgtatacgc cgagtcgcct tttagctcac tgcagtccgc aaaagagaaa
      121 accacggtat tacaagattt acgtaaaatt tgcacgccac atgcgtcatt atcagatgaa
      181 gcgtgggaaa agttaatgtt gtcagatgaa aacaataaac aacatatacg cgaggccatc
      241 gtggcgatgg aacgtaataa tcagagcaat tattgggaag cgttgggtaa ggtagaatgc
      301 ccggatatgt aagaacagca acaactactg accaacgctt ctttacctga tatcaggatt
      361 tcttcatttt cacctttgca gtaaccaaca atgcagtcag caacatcaat gtgccggaca
      421 acatcaacgg cgaagtcaga ccaatattat ccagcgcata accgccgatt gccgcgccac
      481 aggtattagc aagctgaata accgccacct gaatagaccc ggctttttct gcctgatcgg
      541 ccagcgagcg ggtgatccac gttgaccagc cgacgggaac caatgcaaaa gttagccccc
      601 agataatcgc cacgccggta gcaacgattt tatcgcttcc ccacaacgtc agtaccaacg
      661 cactcacagc cagtattaac ggcgcgcctg ctaaggccag ttttaccgaa cgtttaagaa
      721 tgaacgacga aagcgacgta ccaataaagc tggcgatacc aaaactcaac agcaccagcg
      781 ttaagccatc cacgccgaat cccgccaggt tcatatacac cgggcgaata tacgtgaaga
      841 aagcaaactg cccggcgaaa gacatgaaga tggcgatcat ccctgccatc acacccggac
      901 gttgtaataa gcggaaagta ttttgtttct gatgcgaggg ttcgcctggc agtgaaggca
      961 atgatttgat aatccagaaa atacacagca cgcccatcac cgccgccgca ttaaagacat
     1021 tgcgccaacc gataagctcg cctaaaaaac tgcccaacgg cgcggcaatc accagcgcaa
     1081 tagaaaccgc gccgaagatc accgacagcg ccttcggcac cgtacgcggc ggtaccagac
     1141 gcatggtcag cgacgccgac atcgcccaga acccgcccag cgccagcccc agacaggcac
     1201 gaccgattaa aagcaaactg aatgagttag caaaggaaac cagcaagcag gagagcgtca
     1261 gcaaaacggc aaacaaaata acaacgtagc ggcggtcagt ggcctgaatt gtctgggtaa
     1321 taaacaaact ggcaaacatt gccacaaagg cggtcacggt caccgattgc ccggcaaccc
     1381 cttccgaaat gcctaaatcc tgggccattg gcgtcaacaa actgacgggc aaaaactcaa
     1441 cgataatcag acaggcgaca caaaacgcca ccgagaaaac ggctgaccag ttcggtcggg
     1501 tgatggcatc cgcgccgcgg ttttcggcaa taaattcact catggtgtta cccgtggcat
     1561 gtttcagggg aaaagccgac cactttaaca ttgaaatgtg tgacgaattt aacgtttttg
     1621 caacttttac ggtgttgctt gagctggagt taagatcgaa acggaacagg gcaaagatta
     1681 ccggatgcgg cgtgaacgtc ttatccaccc gacaggtcag gcgcggtctg taggctgata
     1741 agacgaaacg gtttcaatgc aaccagtgaa cgccctcggc aggctgaaag aacgcgttat
     1801 agcgcatctg aaaagcatta atatcctgca tttcaggttc ggtttcacgc agaaaaccca
     1861 gcgccagcct gacctgagca tcggtaatcg gcgcggaaag gcgggctttt tgtaaaatcc
     1921 ggtgccattg cagcaggttc tgttcgctat catcgacaat gacaatttgc cagataagca
     1981 acacctgcgc ggcgttttgc aggtgcgact catccgggcg atgcaaataa cgtaaaaatt
     2041 cgtcaccggc tgactttccc atttgatcga ccatcgactc cacgggtacg acatcgatat
     2101 tcagccgatc gctgagacgg cgaatcgctt ttctggcccc ggatgtcagc acccgaaaac
     2161 caaccacaaa cactaccgcc agcgtggcga gcattatcca gatcatgagt tctcctgcgt
     2221 aatcagaact catgatatct ggaaaccctc gccagttact aatccaccag aataatcttc
     2281 aatgcgaaca gagctgcgac caccaccaca cacaggttca gatcgcgcca gcgcccggtg
     2341 cacactttca tgatgcagta cgacataaag ccgagtgcaa tcccttcggt gatcgagaaa
     2401 gtaaagggca tcatcaccgt ggtaataaac gcaggcaccg attcggtaaa atcatcccag
     2461 ttaacgcgcg ccaggctcga agtcatcagc acgccaacaa agattaacgc tccagcggtt
     2521 gcgtaaggag gaaccatcgc caccagcggt gagaagaaca taactaacag gaacataacg
     2581 ccgaccacga cagccgttag ccccgtgcga ccaccgactg ccacaccaga agtactttca
     2641 atataggcag taacagacga ggtaccgata aatgcgcctg ccaccgaact gacgctatca
     2701 acatacagcg ccttattcat attggggaat ttaccgttgc tatctattaa gcccgcttta
     2761 tcagttacac caattaatgt tcctgatgaa tcaaataggt tgatcagcat aaaggagaaa
     2821 atgataccgg cgagttcaag tgttaacgcg ccgctcaaat ctacttcacc aatgacgccg
     2881 ctaatatcag gcggaatgga atagacgccg ctaaaatgaa catcaccgaa aaataatcca
     2941 cagcaagacg tcaccacaat agaaaccagc accgcggcat gaaaatgacg tgatgacaac
     3001 acggttataa taaaaaaccc taaaatacct aacaacacgc cgtgagaact taaatcgcca
     3061 atcatcacca gcgtgtcttt attggcgaca ataacaccag tgttttttaa tcccattaag
     3121 gcaataaata atccgattcc actggtgata ccaatacgta aacttaatgg aatgttggag
     3181 atcatccagt accggatacg aaacagcgtg agcaaaaata gcccaattgc gccccagaat
     3241 atcgcgccca tcccggtctg ccaggagatc cccatcgccc ccacgaccac gaacgcaaag
     3301 aaggcgttca gccccattgc cggagccagc gccacgggta agttagcaaa tatccccatc
     3361 gcaatactgc cgataccggc aatcaaacag gtggtaacaa acaccacttt cgggtccatt
     3421 tgtgccgcgc cgaggatttg cgggttcaca aaaacaatgt acaccatggt taaaaaagtg
     3481 gtcatccccg caatcaattc tgtgcggacg gtggtcccat gctgaggtag tttaaataat
     3541 cgcgaaagcg tccctgattc attactcacg taatcggtat tgtcattatt catttttttg
     3601 tccattggag gagatttaat cccttgcttt tgtaaaacaa atgacaacaa cggttcagtg
     3661 ataattatca catttcaatt gcacattaat agatattctt taataatctc gcgacgtttc
     3721 tttatgataa ataataatca aattgataaa atcaaaatga gaaaaatatg aataattcca
     3781 ttaaccataa atttcatcac attagccggg ctgaatacca ggaattgtta gccgtttccc
     3841 gtggcgacgc tgttgccgat tatattattg ataatgtctc tattctcgac ctgatcaatg
     3901 ccggagaaat ttccggccca attgtgatta aaggacgtta cattgccggt gttggcgcag
     3961 aatacgctga tactccggct ttgcagcgga ttgatgctcg cggcgcaacg gcggtgccag
     4021 ggtttattga tgctcacctg catattgaat ccagcatgat gacgccggtc acttttgaaa
     4081 ccgctaccct gccgcgcggc ctgacgaccg ttatttgcga ccctcatgaa atcgtcaacg
     4141 tgatgggaga agccggattc gcctggtttg cccgctgtgc cgaacaggca aggcaaaacc
     4201 agtacttaca ggtcagctct tgcgtgcccg ctctggaagg ctgcgatgtt aacggtgcca
     4261 gttttaccct tgaacagatg ctcgcctggc gggaccatcc gcaggttacc ggccttgcag
     4321 aaatgatgga ctaccctggc gtaattaacg ggcagaatgc gctgctcgat aaactcgatg
     4381 catttcgcta cctgacgctg gacggtcact gtccgggttt gggtggcaaa gaacttaacg
     4441 cctatattgc tgcgggtatt gaaaactgcc acgaaagtta tcagctggaa gaaggacgcc
     4501 ggaaattaca actcggcatg tcgttgatga tccgcgaagg gtccgctgcc agcaatctca
     4561 acgcactggc accgttgatc aacgaattta acagcccgca atgcatgctc tgtaccgatg
     4621 accgtaaccc gtgggagatc gcccatgaag ggcatatcga tgccttaatt cgccgcctga
     4681 tcgaacaaca caatgtgccg ctgcatgtgg catatcgcgt cgccagctgg tcgacggcgc
     4741 gccactttgg tctgaatcac ctcggcttac tggcacccgg caagcaggcc gatatcgtcc
     4801 tgttgagcga tgcgcgtaag gtcacggtgc agcaggtact ggtgaaaggc gaaccgattg
     4861 atgcgcaaac cttacaggcg gaagagtcgg cgagactggc gctgtctgcc ccgccatatg
     4921 gcaataccat tgcccgccag ccagtttccg ccagcgactt tgccctgcaa ttcacgcccg
     4981 gaaaacgcta tcgggtcatt gacgtcatcc ataacgaatt gattacgcac tcccgctcca
     5041 gcgtctacag cgaaaatggt tttgatcgcg atgatgtgtg ctttattgcc gtacttgagc
     5101 gttacgggca acggctggct ccggcctgtg gtttgctcgg cggctttggc ctgaatgaag
     5161 gcgcgctggc tgcgacagtc agccatgaca gccataatat tgtggtgatc ggtcgcagtg
     5221 ccgaagagat ggcgctggcg gtcaatcagg tgattcagga tggcggcggg ctgtgcgtgg
     5281 tacgtaacgg ccaagtacaa agtcatctgc cgttacccat tgccgggctg atgagcaccg
     5341 acacggcgca gtcgctggcg gaacaaattg acgccttgaa agccgccgcc cgtgaatgcg
     5401 gtccgttacc cgatgagccg tttattcaga tggcgtttct ttctctgcca gtgatccccg
     5461 cgctaaaact aaccagtcag gggctatttg atggcgagaa gtttgccttc actacgctgg
     5521 aagtcacgga ataataaaaa aagcccggcg tcatgccggg caaaagtcac cagttacgtt
     5581 tatgccactg tcaactgctg aatttttttc tcgcggcgga ttttgcgttc ttccattacc
     5641 gccactatcg ccatcaggca gatacaacca atcgcggcga tatccagcgc ggcgaaggtg
     5701 cctgcccagc cggtaaggcc gaataccggc gtcccatcgg caatcattcc cagacctaac
     5761 ttggcaaagc tgtcaccaat caggtaagca aaggtgcctt taataccatc ggcagcgcca
     5821 atcgcttttt taggtacaaa gccaacagca gccacaccaa tcaacaattg cgggccaaag
     5881 accaggaaac ccaacgcaaa gagagaagcc agatagatat attcgttact ggcatgttga
     5941 tacacaccga gcgtggcgat aatcagcgcc agcgcgatgc aggccaccag gccacggcga
     6001 ccgttcgcca ggtcagagag ccagccccac agcagcgtac cgaccagcgc accagcttca
     6061 aacagcgtaa agccctgaat cgccaccgct ttagagagtt tcagttcctg gaacgcgtat
     6121 acggttgacc actggtcgat accaatacgt accacataga ggaaaatgtt ggcgaagcac
     6181 agcagccaga tcactttgtt tttcagcaca tactcaacaa agatctgcca cttggtcata
     6241 tcggtagatt ctgtctcttt gtcctcttcg ctgatctcct cgccgaacag ttcttcagct
     6301 ttgccgaggc cataagattc cggggagtcg ctgccgtaac gcaggccgat aaaaccgaca
     6361 atcagcgcga taatcgacgg gaagataaac atgccgatga catggccatc gaacaggtaa
     6421 tttgccccga acagcgccac acctgctgcg cctgcaccgc caaggttgtg agaaatattc
     6481 cagaaaccga ggaatgtccc gcgtttacga cgcggcgtcc atttggtgat ggtggagtaa
     6541 ctgcacgaac cgccggtact ctggaaaaag ccgcttaagg cgtagaaggc aatcatcagg
     6601 aacaggctaa ccgagccgct gcccatactg gcactgaagc ccagcataca aatggcagag
     6661 aggataagca tgaacggcag gaattgtttg gtgtttttgc cgtcggcgta gtatgaaacc
     6721 aacgttttac ccacgccata agtgatggag aaacccaggc cgatcatccc cagctgcgtc
     6781 atgctcaacc cgtaggtcga aatcatatcg ttctgcgcga tgttaaagtt cttgcgaatc
     6841 aggtacatcg tcaggtagcc gataaagacc accaggtagg attgcatgaa cggtttgaac
     6901 cacattttgc gccgcacttc gagcggaagg tccagggtcg gcttgcgaac ttggtttagg
     6961 aaagccagca tgggttactc ctgaaatgaa tacctgcgtg aggcatgcat tgtaaaaatc
     7021 agcagcaaat caggcctgag atagcgtcca agaaaaacct ggaaaatttc ttagttttgc
     7081 cggatatgaa gtgaaaaggt gagatgcatc acgcttcacg cggtgtctgg gcgttcaaaa
     7141 agggcagtaa cagcagtgcg gaaatcccgg cggcgataga gataaccaca aaaaatccgc
     7201 tccagtgcca ggtatcgagc actttcgcca gcggccaacc agcaagcgac gcccccagat
     7261 aagcaaacaa gccgacaaac cccgtcgccg cccctgccgc ctctttgtgg gaacactctg
     7321 ccgccgccat accaattaac atctgcgggc caaagacaaa aaaaccaatg gtgaagaagc
     7381 aggttgcctg catcacgtag ctggcaaatg gcatcagcca cagcgagccg actgaaagca
     7441 aaattccggc ggcgaaaatc aaattcatcg gcccacggtt gccgttaaac aatttgtccg
     7501 agccccaacc ggctaccagc gcaccgataa atccgcccag ttcaaacatc gtcactgccg
     7561 tattcgccgt gaccagatcg acgcccagcg tctcggacat atacaaattg ccccagtcgt
     7621 tgatcgccgc ccggaccaca tagaccagca catagcaaaa cgaaagcagc cagatatacg
     7681 gattcagcaa cacatatttg gtgaggatct ctttacgcgt caaccctgcc ccttcttgtt
     7741 gttgagcaat ttccagcgcg tcgtgccgcc attcaccgac cgccggtaaa cctaacgcct
     7801 gcgggcgatc gcgtagccgc cagcagagaa aaatccccac gactatcgcc atacaaccag
     7861 caatcatcat cccggcacgc cagccgtaat gcagcgcgct cgctgccatc acaatgggaa
     7921 tgagtgcgcc gccgacgtta tgcgccgtgt tccataatgc ccaccaaccg ccgcgctcgg
     7981 tacgtgaata ccaggccgtt aacagacgcg cacacaccgg tgaaccccag ccctggaaaa
     8041 aggcgttcag cacccagagc acggcaaacg cccatagcga cgtcgagaag ccaaacagaa
     8101 tgttcatgat gcccgtggcg ataagcccta tccccataaa ataacgggca tttgagcgat
     8161 cgctgacaat gccggagaca aacttcgaca cgccgtaagt aatgtaaaac agggtcgcta
     8221 acaggccgat atcgctacgg ctgagcacgc cgttagcaag gatttctggt accgcggcgt
     8281 taaaactttt ccgcgtgaag taaaacagcg cgtaacccag ccagatggtc agcagaatat
     8341 gccgacgcca gtagcgataa cgggcatcaa tttcatgttt atcagtcatt aatggcgcat
     8401 cggcaggcgc tttcagaaac ggcaacatca tcgaacctta gacataacgt tgaggtagag
     8461 aaacgctgac acgcgtgccg tgcagacagg aaatgtgtaa tgtgccgccc agcgccgtta
     8521 cgcgctcgcg cattccggtg aggccaaaac cttgttgccc ggaacccggc ggcaaaccgc
     8581 tgccatcgtc ttcaatcacc agcatcaacc gttcatcctg ctgccagcct tgcagcgtaa
     8641 ccgcgctggc atcggcatgt ttcacaatgt tgttcagccc ttcctggcag acacgaaaca
     8701 gcgtcacgcg ctggttttcg cttaatgctg attcatcgat acgccattcg agatggctga
     8761 caatgccgcg cccttccagc tccatttccc gcatcagtga gcggatggcc tgctccaggg
     8821 tgagatcatc cagctggcgc ggacgtaacc gtcctaacaa acggcgcacc gcgtcgtaaa
     8881 cgcccagcga cagttgttcg atgagctgcc cgctctgctt cacactggcg ttatctgccg
     8941 ccagccgctg aacaatgccc gcctgagtac gaatagcagt gatggtctga ccgatatcgt
     9001 cgtgcagctc acgcgccacg tcgcggcgca cgctctcttc ggtttctaac aaacgttcag
     9061 ccagatgctg attgcgcgcc agttcctttt gtagcgactg gttaagttca cgcaaccgct
     9121 ggatgcctgc gcccagtaac aaccctgtca gactttgcac cagcaacgag agtaataaat
     9181 ccaccagatg atcgcgccag gtttgactgg cgatcagcgc gatggcgttc atcaacgtcg
     9241 caatcagcgc tccttgccag ccatagtgcc aggccagcgc gataatcggc agcgccagac
     9301 agaatggcgt aaagcgcgac agttcgtccg gcaatcccaa ctggagccag agactgataa
     9361 caaacagcag caagtaccag accagatgcc gcccgcgcca gttgatgggc tgagaaacca
     9421 gtgacgggcc gagcggcagc caggtgttat tggcgagata gtgccagaac accagacata
     9481 tcggggccag cgtcagaccg ccagttaaag tcagcaacag cgcattccac gactctttgc
     9541 cgtgccaaag ccagggcagc gactgcaaca acgccgccgc cgttaacgcc gcaccctgta
     9601 acagcaaggt gcgccagtca cgctgatggc gatagcgcga gatcagcgct acgggcagta
     9661 acgtcagcaa actaccgatc atcaataacg gaaaatgggt taaaccgacc gcctgcgtta
     9721 gccagtaaat cagcagccac tccgcaccca gcaatacggg ccagtatccg cgcgggcatt
     9781 gcagcattag ccccagacgc agaccaaacg gaaataacag caccgccata tcagggcgct
     9841 caaccagatg caggctgata ctccataggc aaaaccatgc ggcagagaag ataaaaaagc
     9901 aggcaataac ggtaattaag cgggagaaca acgtcttcat caccagccat caaacatgcg
     9961 gcgcgccagc tctacgtcgt tactgacgcc cagtttttcc atcagattgg cgcgatggac
    10021 gtgtaccgtt ttcggtgaca agcccagttc ggcggcaatc tctttcaccg ccattccttg
    10081 cgccagtttt tccgccacct ggcgttcacg tttggtcagc gggtcctgac gaccggatgc
    10141 cagtttaatg gcaatatccg gcgtcagata acagccgccc gtggcaaccg tatgcaccgc
    10201 agcaatgagt tcatccgggc tacagcgttt ggaaagaaag ccgcgtgccc ccgcgttaag
    10261 cgcctgctca accagcgcag gactgtcgtg aacggagagc ataatcgtcg ccataccttt
    10321 cggcagctgg cttagcagct ccagaccgga gatatcgggc atggagatat cgcaaataca
    10381 cacctgcaca ccgcgccccg gcagccccgc cagcgcctcg cgccccgaac caaactcggc
    10441 aactacctgc aaatcaggtt ccagccccag cagctgcgca aagccggagc ggacgatgag
    10501 gtgatcgtct ataagggcaa cggtgatcat ggtcttgtcc tggcgggtaa aaaaaaacgc
    10561 gcttacctta acgataagcg cgatgttgtt caagccttga gcggttactg aaaaaacacc
    10621 gcgatcttgt taaacatcgt cggatcggac tgattacgct gcactttcac gacatcttcc
    10681 agcttagcga tttggcttat catctgctcc agacgctggt cgtcattgac cagtagccag
    10741 atatggcttt tgtcgctgtc ctgaatcggc agacaaagaa tgccttcaac gttaaaagcg
    10801 cggcgggcaa aaaggccaca aacgtgggtc attacgcccg gatggttgcg aacggtgagc
    10861 tccagaatta cgttgttatg agttgtgttt tgcatggctt attcccccac catttcagta
    10921 ttcgccgcac ctggcggcac catcgggtaa actttttctt cggcatcaat gcgcacatgg
    10981 atcagcgccg ggccagggcg attgatgatt tcctgcaatg cagcctgcgg atcggcttcg
    11041 ttattcaaat cacaggtttc gaggccgaat ccggcggcaa tctgcataaa gttgattttg
    11101 cccggatagg tggcggcaaa aacgccttgc tcgtagaaca gactctgttg ctgatgcacc
    11161 agccccagcg cttcgttgtt catcagaatg attttgacat ccagctgatt ttcactggcg
    11221 gtcgccatct cctgaatatt catcatcagg ctgccgtcgc cggagaaaca caacactttg
    11281 cgatccgggt tcgccagcgc cgcgccaatc gccgcaggca ggccaaaacc catcgtgccc
    11341 agcccaccgg aggtcagcca ctggcgtggg cgattgagcg gataagcttg cgcggtccac
    11401 atctgatgct gacccacatc ggtggtgata atcgcattgt catcgacaca ggcggcaacg
    11461 gcgttgatca ggccgtaatg gcttaatgga tcgcacgctt tcgggattgg acacgggaat
    11521 tcgcgctgca aatccgctac caactggtgc cactctgcac gcggttgcgc ttccacccgc
    11581 gggatcaatt gcgccagcac gtcatcaaca tccgcctgaa tcgccacatg cggctgcttg
    11641 attttaccca gctctgcgcg atcgatatcg acatgaataa ttttggcatt cggacagaac
    11701 tgctcggttt tgccaatcgc ccggtcatca aaacgcgcac cgagcacaat caacaaatcc
    11761 gcctcctgca aaatatagtt ggtactgcgc acgccgtgca tccccagcat acccagcgac
    11821 aacggatgcg cttttggcag catgcccagc gccattaaag tcatggtggt aggcagttgc
    11881 gctttctccg ccagttcacg cacccgcgca ggcgcattga tcacaccacc acccagataa
    11941 agcaccgggc gtttggcagc gttaatcatt gtagctgcgt cacgaatgct ttcttcgcta
    12001 aaggcgggtg caacggcttt ttctgccacc gcgggctgag cttcaatctc aaaaaccgcc
    12061 gtttgtacat ccttaggaat gtctatccac accgggctag ggcggcctga ttgcgcaatg
    12121 cggaaggcat cgctcatgac ctgtgggagc tcttcgatat gtctgaccag atagttgtgt
    12181 ttggtgatgg ggatagagat gccgtaggtg tccacttctt ggaaggcgtc ggtgccgatc
    12241 atcgaggcgg gaacctgacc agtgatgcaa atcagcggga tggagtccag ccgcgcatca
    12301 gcaatggcgg tcaccaggtt agtcgcaccc ggcccgctac aggccataca gaccgccggt
    12361 ttaccgtcgg tgcgcgccat tccctgagcg ataaaccccg cgccctgttc atggcgagcc
    12421 agaatatggc ggatttgcgt gctttggctt aaggcgtcgt aaacaggcag gatagaaccg
    12481 cccggaatgc ccgtcacaat cttaatgccc tgctgttcca ggaaatgaac gataaattct
    12541 gcgccggtaa agcgcttacg cgtcgatgtt gtgcccgaac ttgccatgct ccagtccttt
    12601 tcttctgggc cgactttccg ggaggtgctt aaacgaaaaa ccccgcccgg tttgcgccgg
    12661 cggggttttg gaatcgtgtg ttgttccagt ccctacggcg cattgccgac gaccaccacc
    12721 acacgcacga cgaccactgc ggcggatggc gcagttggta gtagttttgc gttgaacatg
    12781 gaagtagtca ttggggacct tgttggtttt gtgtttaaca atatttatac aagcacagct
    12841 ttacagggga gacaatggaa aatttttcag caagggaaaa ttgaggggtt gatcacgttt
    12901 tgtactgaat tgcagataac aaaaaacccc gccggagcga ggtttcgtca gccgcctgcg
    12961 gctggtaacc gcaaagcaca ctgtattatg tcaacactga aagtatacgt gttccgcgca
    13021 gaacgcgcaa tttcggcacg aattttgacg tatttagtgc atagttgagt atcgttcaca
    13081 gtttgcgttt tgtccaaata ttactgttta tttatacagt aagcttctat aatatcactg
    13141 tacgcaatgt gttatgcggg ggccgcatcg ttacccggcg cactaagtcc tggctgaaac
    13201 gggtggtgcc gtcagcgcct taaccccgcg tgagcacact gtgttatgtc aacaagcaca
    13261 acgtttctcc ttgagatacc gcgtgcacaa cagctggcaa caggcagcgg aaaggtacgt
    13321 cagctggcag tgctcctgaa ccacaggaga cgcgtatgag cctggtggat atcgccattc
    13381 ttatcctcaa actcattgtt gcagcactgc aactgcttga tgctgttctg aaatacctga
    13441 agtaattcag attcaagtcg caccaaaggg gagcgggtaa ccgctcccct tttatattta
    13501 gcgtgcgggt tggagtcgga tgcgatgctg acgcatctta tccgccctac catccctccc
    13561 ggcaacattt attgccgctt ttgtttacat attctgccgc taaacaattc cccattcctg
    13621 gcgtatatct ggctaacatt catcaatgtg atagattcct ctcccgcatt tatgggaatg
    13681 cgtagtgact tattctaatt atttttataa aagcatccgt gataatgaaa aggcaaagaa
    13741 acgtcaattt gttattgatg ttggtattac tcgtggccgt cggtcagatg gcgcaaacca
    13801 tttatattcc agctattgcc gatatggcgc gcgatctcaa cgtccgtgaa ggggcggtgc
    13861 agagcgtaat gggcgcttat ctgctgactt acggtgtctc acagctgttt tatggcccga
    13921 tatccgaccg tgtcggtcgc cgaccagtga tcctcgtcgg aatgtccatt tttatgctgg
    13981 caacgctggt cgcggtcacg acctccagtt tgacggtatt gattgccgcc agcgcgatgc
    14041 aggggatggg caccggcgtt ggcggcgtaa tggcgcgtac tttgccgcgt gatttatatg
    14101 aacggacaca gttgcgccat gctaacagcc tgttaaacat ggggattctc gtcagtccgt
    14161 tgctcgcacc gctaatcggc ggtctgctgg atacgatgtg gaactggcgc gcctgttatc
    14221 tctttttgtt ggttctttgt gctggtgtga ccttcagtat ggcccgctgg atgccggaaa
    14281 cgcgtccggt cgatgcaccg cgcacgcgcc tgcttaccag ttataaaacg cttttcggta
    14341 acagcggttt taactgttat ttgctgatgc tgattggcgg tctggccggg attgctgcct
    14401 ttgaagcctg ctccggcgtg ctgatgggcg cggtgttagg gctgagcagt atgacggtca
    14461 gtattttgtt tattctgccg attccggcgg cattttttgg ggcatggttt gccgggcgtc
    14521 ctaataaacg cttctcaacg ttgatgtggc agtcggttat ctgctgcctg ctggctggct
    14581 tgctgatgtg gatccccgac tggtttggcg tgatgaatgt ctggacgctg ctcgttcccg
    14641 ccgcgctgtt ctttttcggt gccgggatgc tgtttccgct ggcgaccagc ggcgcgatgg
    14701 agccgttccc cttcctggcg ggcacggctg gtgcgctggt cggcggtctg caaaacattg
    14761 gttccggcgt gctggcgtcg ctctctgcga tgttgccgca aaccggtcag ggcagcctgg
    14821 ggttgttgat gaccttaatg ggattgttga tcgtgctgtg ctggctgccg ctggcgacgc
    14881 ggatgtcgca tcaggggcag cccgtttaag cgcacgtcac cgcagcatcg tcatcagctc
    14941 catgggagaa cgatgctgct ttatcagatc acgcatcacc cgcatatgcg gtgcggagta
    15001 agaataaaac gcctgatagc ccgcacaaag cacgctgtct ggcgtctcct gccgagcgtg
    15061 cgggcacagg cgatgccagt cgcacgcctg gcagtcacgg cgcggttgcg ggttttcgct
    15121 gccacaccat ttttgtaagg tggcgtcaaa aagctcaatc gagatacgac tgatatctgc
    15181 ccgcacccaa cactcaaaca gggcaattaa aaatttcccc cacagctctg ctgtcaccga
    15241 agcggccgtc agatcgcccc ggtcatcaaa ttcaagatag ggaatgagct gtacggagcg
    15301 gatgccccgc tgtttgaaat tgtgataaaa atcggcaggt tgcagagcat ccttgtactc
    15361 tacaaccagc ttatgcctgt cctcttcagc atcaatgacc tgacttcctg tcatgcgact
    15421 ctctcaataa atacgataag ttgatgtata tgcgttacag caaacagtat tgcgagagat
    15481 aacaccgcga gggagatcaa aaatttgtca cggactacgt gaaattggga aaaatcgcta
    15541 ttcgtgacat ccattaaatt gcgattacgg gtgtagtgcc agaggatcag cgccacgatg
    15601 gcgaggatgc caatcgaaat ccagaataac atgcccgcct ggtgccagtt gtgtttgata
    15661 gccaacgcca tcaacgcgcc gtagcccagc atggtacgaa accacgccag tgatgtgcgc
    15721 tccggctgca gccctgggtc ggcaatgcgt ctggctttac ggctatccgg catacaacac
    15781 cagtcccata acgatcaccg ccacaaccat caatattaag ctgatgatta ataagctgtt
    15841 ggtgtagggc aggtcctctt tcagacgcat cgctttttca ttgcgtaacc agcgcaggta
    15901 gccatacatt gctaacccgc cggaaaacag acacaacaac aacgccagca gttcacgaat
    15961 gacgggcgtg gcgaaatctg gcgcaagttg atcaagccca acccccgccg ccagaaatcc
    16021 tagtgcggta cggatccacg ccagaaaagt acgctcatta gccagcgaga agcggtaatc
    16081 gggcgcttct ccaaggcggg aaattttcat tgttgctcct ttgcaatttc actaacgctc
    16141 tatctttagc ggtttttttt aagaatttcg tccgcccgtt tcctgatatg cttttgccat
    16201 ccgctacaag gaggtcagat gggtatcata gcgcaaaata aaatcagttc attgggcatg
    16261 ctgttcggtg ccatcgcttt aatgatgggg attatccact tttcattcgg ccctttttcc
    16321 gcaccaccgc caacgcttga aagcatcgtg gcggacaaaa cggctgaaat taaaagaggt
    16381 cttctcgccg gaataaaagg cgaaaaaatt accactgtgg agaaaaaaga ggacatggat
    16441 atcgataaaa tcctcgatca aagcggtatc gcactggcca ttgctgcgct actttgcgca
    16501 tttattggcg gtatgcgtaa ggaaaatcgc tggggcattc gcggcgcgct ggtttttggt
    16561 atcggcatcg tgtgcagcat tttgctgata ttcctgattt tttccttcct gaccggcggt
    16621 tcgctggttt aagcagcctg aataatgccc gccgggtgat catcagcggg cattatcgtc
    16681 agaatttctg ttttttctcc tccaccttca ctccttgcgt cggtaggccg gtgtcatagt
    16741 cgcgtaccac tggcgaatag ccatcttgtg ggcgtgggcg aaacgccccc atccagcgcg
    16801 gtcgtgcatc tttacgccac ggacgcagac tccattggta actgcggaac ggatcgcgaa
    16861 ttttgtccat gtaatccaat aaggcgtcat gcattttgct gcgaacgtct gcaaaacgga
    16921 tatcatcgat caggttatgc atttcatttg ggtcattacg gcgatcgtaa agttcatcac
    16981 tggtgaagag gttgagcacc agtttaaagt catccgtcac ccagcaacgc accggaataa
    17041 aaccgccaaa gctgtcatgc tcaatctcgt agcggttaaa ttccaccatc acgccgcgtg
    17101 gctctttcac ggcaaggata ttttcccccg gcagaatctc tggtttttca atatctgcca
    17161 gcgccatcat tgtcggcagt aaatcaatat gactgactgg cgtatcgacc tgtcgccgct
    17221 ccccttgcgg cgaacggatg atcagcggaa tacgggtgat gtcgtcatac atcgccgctc
    17281 ctttactgat cagcttatgt gcgcccatca tttcaccgtg atcggaggta taaatgaccc
    17341 acgtattttc acgttgctct ggcgttaagg cattgatgac acgtccgatt tggtcatcaa
    17401 caaagtcatt gcaggcaaaa tagagcggat ggtgataaag cccgtcatca ccgactggcg
    17461 atggcatcgc ctgcgcccat aagcggtgat gttccggttt gttcgccagg tcatcctgtg
    17521 atttctcgcc aagttcgtag taaaaatcag cgtatttctc taaatactcc accggacagg
    17581 tgaacgggtg atgcggctca tcatacgaaa ccaccatcag gaagggttcc tcggcgcgca
    17641 cgggctattg cagaaaatct accgctcgat tgctgatgcg gtgcgcccag gtgaaggttt
    17701 cgtcaatatg gttcgcctgt aaatcttcga cgctgtttag gccattgcgc cacaggctga
    17761 tctctttttc cgtcagttcg ctaagatagt tcgccccatc aaaccagtaa tcagcatccc
    17821 actccggcgg acactcgcca gtgccgaaat agtcatgacc gtcgagatgc catttgccga
    17881 tgtaacaggt gtgatagccg gcatccttaa agtagcgccc catagtggag atgtttttgc
    17941 ctggcgcgac gttgttggtc cacgggccgg actggttagc gtagataccg gtgaatagtc
    18001 cggcccgtgc aggcgtacaa accggtgaac aagtgtaggc ggaattaaag cgaatacctt
    18061 ccgccgccag actatcaata ttttgcgtat tcagcggttt accgctatag caaccgacca
    18121 tattggtggc ctgggtatcg gtcaattggg gcgtttcata ggctatcctt cgcgtcggga
    18181 gtaaaggtta cggctgggtc gtgacgatgc caccagctgt caaaaatcag gtagacaatc
    18241 actacggcag cgatgaaata actgatcatc gctaaccagc gcgtaccgta gccgccgaat
    18301 tcagccagcc cgacatagac gccaatcatc gcgaacagga taccaattga cgcgatcttg
    18361 acgtttttcc acggtttcat gtccaccgca aacgcatctt tgaaggtgaa cggcgtggcg
    18421 cgcggtttga taaaaccgat caccagcatc acgaccacgt tgatgcagaa cgtacaggcc
    18481 agcacataga ggaaatggaa gtcgaacttc accagatagt tgatggtgat gtagctgatt
    18541 atgccaatcc ccatcgccac ttttgccgcc agcgccggga tgcgcggaaa gaaaaagccc
    18601 ataatgatga tggtaaccag cggcacgttg taaatgccgt tgagctgttt catccagcta
    18661 tacagcccct gcggcgcgtt cgcgatccac ggcgctacca aaaccgaaac gatagcgata
    18721 aagaaaccaa atttgcgccc gacggtgacc agctgctgcg gctcggcatt ctggttaatg
    18781 atgcgacggt aaatacccat actgaataat gtactggcgc tattcagaaa gccgttgaag
    18841 gtactgatca ccgcaccaaa taacaccgcg ccgaagaaac cctccagtgg cactggcaga
    18901 acgttattga ccagcgtcgg gtaggccatg tcggctttcg gtagatcctg atacagatga
    18961 aacgcaatca accctggcag caccagtacc agcgggtcga gcattttcag caccgccgtt
    19021 aacagcgccc ccttttgccc ttccgccagg ctttttgacg ccagcgtgcg ttgcacgatg
    19081 ccctgatttg tacaccagta aaaggtgttc accagaatta aaccggtaaa tgccgcgcca
    19141 atcggcaagg gatcggtcgg gccaccgact gagtttaatt tctcggcgtg aacggtggta
    19201 atttgctcaa tgccctgcat aaagctgccc ttgcctatcg cgatcaggcc aaataccggc
    19261 accatcaacc cgccgataac cagcccaata ccgttgatgg agtccgccac tgccattgcg
    19321 cgcagtccgc cgatcaccgc atacaaaatt cccgccagac caagcaaaat taccagtagc
    19381 cagatagccg caccgtgaga aatctgtagc gattccccga cgtgaaacag gctgttgagc
    19441 gccaacgcgc cggagtagag aacaatcggc agaaagcaga cgccggtggc aatgaggaag
    19501 cagaagtcga tgataatacg cgtcgtttta tcataacgtt cctccagaaa atcggggatg
    19561 gtggcaatgc cgcgcttcag atagcgcggt aaaaagatta gcgcgaggaa gatcagcgtc
    19621 accgctgaag tcacttccca gcccatcacc gacatgccgc ttttgtaggc ctgcccggaa
    19681 agaccaacca gttgttccgt ggaaaggttg gttaacatta acgaagcggc aataaccggc
    19741 gcttttagtg aacgtccggc aagaaaatag ccttgttgcg atccggtatc tgttttgcga
    19801 accttccacc aggtgatcac cgccaccagc agcgtaaaac cgacaaaact caagatttgt
    19861 aacgaattca tctcatagcc cttatttatg tttatgtccc ggacgcatga atatagggag
    19921 aggtaaaatg cgtcgggtca ggcagtcaga ttcttaaaat acaaacgtcg tatccctgaa
    19981 cggattgttt tctgttaagt tcaggttgtg agcataattc gccggaggga tattaaaatg
    20041 aatggaaaat tgcaaagttc agatgtaaaa aacgaaactc cgtacaatat tcccttattg
    20101 attaacgaaa atgtgatctc cagcggaatt tctctgatct cgctctggca tacctacgcc
    20161 gacgagcatt accgggtgat ctggccgcgg gacaagaaga aaccgcttat cgccaactca
    20221 tgggttgcgg tgtataccgt acaaggatgt gggaaaattc ttttaaagaa tggcgaacaa
    20281 ataactctgc atagcaactg tattatattt ttaaagccaa tggatattca ctcttatcac
    20341 tgtgaaggtt tagtctggga acagtactgg atggaattta cccccaccag tatgatggat
    20401 atttccgttg gtcagcaaag cgttatttat aatggcgaaa tttataatca ggaactcacc
    20461 gaagttgctg agttaataac ttcaccagaa gcaataaaaa ataatctggc agtcgctttt
    20521 ctgacgaaaa ttatttatca gtggatttgt cttatgtccg cagatggtaa aaaagatcca
    20581 caacggcggc aaattgaaaa attaattgcc actttacatg ccagtctgca acaacgctgg
    20641 agcgtagctg atatggctgc cacgatcccc tgtagcgaag cctggttgcg tcgtctgttt
    20701 ttacgctata ccggcaagac gccgaaagaa tattacctcg atgcgcgtct ggatctggcg
    20761 ctatcgctat taaagcaaca aggaaactcg gttggcgaag tcgctgatac gctcaacttc
    20821 ttcgactcct ttcatttcag caaagccttt aaacataaat ttggttatgc gccgtcagcc
    20881 gtgctaaaga atacggacca gcacccaacg gatgccagtc cacacaatta acgtaactct
    20941 ggccagtacg ctttgttggc ctccagcaac tcatccagaa tagctttggc gaccgaggcg
    21001 ctcggtaccg ttttcgacaa cgtaatcgcc tgccacaggt gctgatatga acgctgttcc
    21061 caggcgtcca cgaccagttt ttccaccgcc acttgctgac tcattaaccc tttctgaaac
    21121 tgcgggatat cgccgaccac cagtggttca ggccgtagta gcgcacgcgc atctgtttgc
    21181 gatcttgcag cccaacaatt tgcgccatcc ggctttcaat accgattggc atgtcacaga
    21241 tgttgaggat tttcgcattc gggcgcagac gacgcgtggc ttctgcgaca attgctgccg
    21301 ggttggagta gttgagcatc taggcgtttg gtgaatattt ttccatataa tccaccagtt
    21361 ccaggacgcc gccaatggaa cgcatgccgt acgctattcc gccgggtccg caagtttcct
    21421 gaccaacaac accgtggcgc agtgggattt tttcatccag ttcgcgcatc gggtacttgc
    21481 cgacgcggat gtgcgccatg acaaaatcaa cgtcgctgaa cgccacttca ggatcggtgg
    21541 tgtaactaaa cgcaatgtcc ggcgcttttt ctttaaggat gactttacag gcttcggcaa
    21601 tcacttcctg ccgcgcgcca tcgttatcat aaaatttcag tgcacgaagc gggaaacggt
    21661 cctgattcgc caggagcatc aacacgatgc ctggcgtaaa ggtgcttcca ccgcctgcga
    21721 caaccactga gaatttggtc attatactgc ctccgtaatg gcaacatttt ctgctgattg
    21781 atgagaatta attaagctat cgagctgttc acgcaattgg gatacatgca gaccaatgat
    21841 cacctgaatg gcatcgccac tacggaagac gccgtgcgct cccagctttt taaagacttc
    21901 gtcatccagc gtttgtgaca tgtcatgcag tgcaatacgt agacgcgtcg cgcaattgtt
    21961 aatgctggag atattgccga ccccgcccag ggcttgcagg ataccggcag cctgatccag
    22021 ctcttttttt ggctctgcag cggtggtttg gcctcgcgag gctttgtatt cggcttttga
    22081 gtagagtttc acttccgcat cttcacgtcc cggcgtgcac atgttgaact gcagaatcag
    22141 ggtgcggaaa accacgaagt acagcagggt aaagcacaac ccaatggcga tttgggtcag
    22201 catcatatcc gcgtggttgc tgaacatcgg gatccagttt tgcggtaaaa cctggtcaat
    22261 cagacctccg cccatgttgc ccaccacacc aaagagatac attacggtcg acattgaggc
    22321 cgccagcacg gcgtgtaccg caaacagcaa cggtgaaatg aacaggaagg taaattccag
    22381 cggttcggta attcccacca gcatggcggt taaggttgcg ggaatcagca agcccgccac
    22441 ttttacccga ttttccggtg cggcagtgaa gtacatcgct aaagaaatgc ccacggcacc
    22501 aaagattttt gagttaccgt gcagggcaaa accgccttcc gggaacaacg atttcagtgg
    22561 ctcggcgctc agactgaact cttgcagatg ctgcgcccag tacatctgaa tgccgccttc
    22621 aacagctgcc ggaccaaaga taaacggtcc gtagatgaag tggtgtaaac cggttgggat
    22681 cagaatacgt tcgaggaagg tgtaaaccca caccccaagc gcacccgccg aacgcaggaa
    22741 cgcttgcaga gattcaatcc ccatttgtac ttttggccag ccgagcaatg tcagccaggc
    22801 acaggggatc atcaccagga aggcgataat caccacataa gacgtcccct ggaaaatgcc
    22861 gagaaaaacc ggcagttttt tatcgaacag acggttatgc agcgccgtca caatgccgga
    22921 aatgataatt gcgccgataa tgctggtatc gagggtttta atcccggcca tcattgtcag
    22981 accgctacct gccaccgcgt cctgagtgaa atcgacgccg aagtagcttc cccaggtcat
    23041 tcccatcgca ttgatgaaat agttccaggt caggaaactc accatcaccg ccagacaagc
    23101 acgtccctgc gcttgcttag caaggccaat gggtaaaccg acagcaaaaa tcagcggcat
    23161 attacggaat accgtccaac cgccctcttc aataatgtgt acgatttgcg cgaataaact
    23221 gttcggatcg gtcagtgatt ccccgacaaa catcgggttt tgcagcaaga tggcaagacc
    23281 caccacaatc ccggcgaagg gaaacagcag cactggcgtg aacatcgcgc cgccaaagcg
    23341 ttgaatttga ctgagcattt ccacatcctc ttttctcaat tctgtaagta ggaagcctga
    23401 ttggttatgc ggcagacctg gagcgaggat aggaagatgc gatcggcaga acatgacgac
    23461 ccaatgggat tcgtgatcgc tttcatgctt ttcgttgggc ggtagagatc tacgttttcg
    23521 cgaaaaagta gacatctgtg agcacaggta cacagcatcg aacacatctt taaaaaaaag
    23581 atttttttca atcgattaag cagaacttgt gggcgcatta cccgtgcttg caggcaaaaa
    23641 agagagctag agatgatcta caaaagcatt gcggagcggt taagaattcg acttaattcc
    23701 gcggatttca cgctaaacag ccttcttccc ggtgaaaaaa agctggcgga agagtttgcg
    23761 gtatcgcgga tgactatccg taaagccatt gacctgctgg tagcgtgggg gctggtggtc
    23821 cgccgccacg gcagcggcac ttacctggtg cgcaaagatg tgctgcatca aaccgccagc
    23881 ctgaccggac tggtggaggt gttaaaacgg cagggaaaaa cggtcaccag ccaggtgctg
    23941 atttttgaaa tcatgcctgc gcctccggcc attgccagcc agttacggat tcaaatcaac
    24001 gagcagatct acttctcccg tcgcgttcgt tttgtggaag ggaaaccgct aatgctggaa
    24061 gacagctata tgccggtaaa actgttccgt aacctttcgc tgcaacatct ggaagggtcg
    24121 aagtttgaat atattgaaca agagtgcggg attttgattg gcggtaatta tgaaagcctg
    24181 atgccggtgc tcgccgatag actgctggcg cggcaaatga aggtagcgga acacacgcca
    24241 ctgctgcgga tcacctcgtt gtcatatagc gagagcgggg agtttttgaa ttattcagtg
    24301 atgttcagaa atgccagcga atactaggtg gagtaccatt tacggcgact ccacccggaa
    24361 aaaagttaac cgatactcca gaagagcacc gccagtaatt ggggggtgat aatgcgcagg
    24421 aacatcacta acggatagac agtggcgtaa gagagcgccg ccgcaccact ggttggatga
    24481 agattattag caaacgccag cgccggagga tcggtcatgg aacctgccag catcccgcac
    24541 atggtcaggt aattcatttt ggctaacatc cgcgccagaa tgccaacagt aatcagcgga
    24601 acggcggtga tcagggcacc ataaccaatc cagcttagcc cttcgccatt gaccagggta
    24661 ttcacaaaat ccccaccaga tttcagacca acgaccgaga ggaacagcac gatccccagc
    24721 tcccgcagcg cgaggttggc acttggcggc ataaaccagt acagcttgcc gatactgccg
    24781 atacgcccga ggatcaacgc cataatcagc ggaccgcccg ccagccccag tttcaacgcg
    24841 gccgggaatc ctggcacaaa gacgggaata gaacctaaca atacgccaag cccgatgcca
    24901 ataaacactg gcagcatctg aacctgttgc agtttttgct gcgcattccc cagcacattg
    24961 gcaacggcat caattgcgga cggacgcccc accaggttga ggatatcgcc gaactgcagg
    25021 ctgatatcgc cactggcgac cagttcgacc ccggcacggt tcaggcgcga gataacaacg
    25081 tcatagcgtt ctttaaagtg caggtcgcga atacgttttc cgagcacgtt ttcattggtg
    25141 accaccacac gctcgacgcg caaatcagtg cctttcgtag acagcgaggt atcgacctcc
    25201 tgaccaatca ccagttgcgc attatgtaaa tccgctggct gccccaccag atgcagcaaa
    25261 tcgcccagtt ggataatggt atctggcgaa ggtactttta gggtttcttc tcgtttcaga
    25321 cgcgagcaga taattttgtc gccgttgaga atcggtacat ctttaatggc taaatcatgc
    25381 aggttagggt tctcaacgcg aatattgata gtcttgatca gcgcgccgcc attggtgcgt
    25441 gaagactcgt gctgctgagc ttctgtctcg acattgacgc ggaaaataac ccgcaacatc
    25501 cacatggtga acaaaatccc gcaaatgccg aatggatacg ccatcgcgta actcatcccc
    25561 atctgatcga ccatttccat tggtgtaccc aggtcgcgca agatctgctg ccctgccccc
    25621 agcgctggcg tattggttac cgcaccggag aaaatcccca gcactaccgg cagtggaata
    25681 tcaaacagtt tatgcaggat ggcggtaacc agaccaccga tgatgacgat cagaacagca
    25741 aacaggttga ggcgtaatcc ggagacgcgc aatgaggcaa agaagcccgg ccctacctga
    25801 atcccgatgg tataaacgaa caggatcagg ccaaattcct gaataacatg cagcatatcg
    25861 ctacttaatg tcatccccgc ctgagaaaca aaatggccga cgatgatccc accaaacagc
    25921 acgccgccaa tacctaatcc tatgccgcga aatttgacgt tgccgataaa caaaccgacg
    25981 actgccacca aagccagaat actgaccgtt aatgctatat cactcatcgt ccatttctct
    26041 tgagataaca ttttaattgc tacggattat ggctgaatcg gtgaagcaga tttgccttat
    26101 agcgcacatt atcacgggaa agaacgtgcc gaaatatctt aaacagtcga aatttaacgc
    26161 attgttgaag aagaaagaca aaaaaagccc caccagaatg gcggggcaaa gagaatagct
    26221 agttagctat ttaacgcggg acgttcgctg atagcgatac gctgcgctgc gatgggttca
    26281 ggctcattac gaattaaatc aatatgcagt aaaccgttta cgaaggttgc gccagagact
    26341 tccatatttt cagccagcgt aaaactcagg ctaaatggct gattcataag cccttgatgc
    26401 agccattttt tctcttcttt tggctgctcc ggcgtgcctt ttacgctcag gcgcgtgcct
    26461 tccagttgaa tctctaaatc ttcctgacgg aaacctgcca gcgcaagggt aatgcggtag
    26521 tggttatcgt cgcttttctc aatgttgtac ggcgggaagc tctggctttc accggcgttt
    26581 tgcagtgcgt tggccagttt gtcaaaaccg atccattgac gcatcagtgg ggataaatcg
    26641 aagttacgca tagtcatttc tccttctaag aagcgagtaa gtacctgcaa atccgaagat
    26701 tcgcatatgc tccctgacgg cgagcatgga gatgtcaggc cgcgccaggc ggccttaggg
    26761 aattagttga tttcgatacg gcgcggtttt ttcgcttccg gaatcacgcg ttcgagatcg
    26821 atatacagca aaccatttac caggttagcc ccacgaacat gaatgttctc agctaactgg
    26881 aatttgcgtt caaagttgcg ttcagcgatg ccctggtaca gataggtgcg ctctttttgt
    26941 tcgtcggcgt gagcaccttt caccaccagc agattatcct gggcggtaat ttccagttcg
    27001 ctctcagcaa aaccagccac agcgatagca atgcggtaat ggttttcgtc taccagttca
    27061 acgttatacg gagggtagcc gccattactc tggctctggt tgttttctaa gtggttaaac
    27121 aaacggtcaa atccaatagc agaacggtaa agcggggata aatcaaagtt acgcataatc
    27181 aatagctcct gaaatcagcg agaatgtaag accttccaca atggacaggt caggtagcca
    27241 gaacacccat caggcgcgtc ctcatcgact acgatgtaaa atgggtctgg aaatgaactt
    27301 ttcaagcctt atcagcggac ttttttttgc agtttatggt ctattgcata gactgagggg
    27361 gcagcacgaa acgttaaagt gcgatagccg ccacagtgcg acgtaatgat gatgttattt
    27421 tcagcaaggg atcgctataa ctcatcaggc caacacccac gatcaacaga tgaatttatt
    27481 atgataagaa atgtgttgtt agcgttcatg atatgcagcg gaatgacatt actcggagga
    27541 tgctccagcg tgatgtccca taccgggggg aaagaaggaa cgtatcccgg cacccgcgcc
    27601 agcgccacca tgattggcga cgatgaaacc aactggggca ctaagtcgct ggcgattctc
    27661 gatatgccgt ttaccgctgt aatggacacg cttttactgc cgtgggatgt gttccgcaaa
    27721 gacagctcgg tgcgctcgcg cgttgaaaaa agcgaagcca acgcgcaagc caccaatgcg
    27781 gttattccac cggccagaat gcctgataac tgatttagcg atcagtttcg gtgatccaca
    27841 gctggccacc ttccatccac gccagccatt gaccatccgg cgagaagacc acggcgtccg
    27901 cagaaggtgg attggcgtgg ttttcggtta aatactcaac ctcgccgctt tgcgcatggg
    27961 cacaaacaat tcgattatcc agcacaaagc ccaaccattc tcctgacgga tgccagttaa
    28021 atgcagactg aatatccgtt ttgttatggg ttaacttgcg cggttcgccg ccctgtggcg
    28081 agataagcca cagttgcaca atgccgttat catcacgcat taaaaacgcg atttgcgtac
    28141 cctgcggatt acagcgcacc cagtggcgcg ggacgttgac taaccccgga taagcccgat
    28201 gatgggtaaa ggttaaacgt cgctgcacga cgccacgcgg tggcgcgggt agggttgttt
    28261 ccgttccact taacggcgca tcacctgccg atttccagct agcttcatct tgcggtaact
    28321 caacgataaa tagctccggc actttctcgc cctttggcga aagtgtgtcg ccaataaacg
    28381 ccagcgcgtg atttcctacc catccttctt cataagcacg attgatttca tcactgccag
    28441 gctgcggcgt gggcgtggtt ttactcacca gcacacacca gtggctaccg ctgtattcac
    28501 gcggatgttg cttttgtacg ttgaccgggc caaacggtgc agcaaccccg acgtttcgca
    28561 aatccagcgc cggatcgagt tgatgcatta catggtcgtt ataggtaaag ctcccccttt
    28621 caccgttcgg gctaaagaca tgcacatggc tgccaccgcg cagcgctcct ggcgtatacg
    28681 gcgcggtaat atccattgca tcgagattgc tcaccttgcc gccttcagca atgactccgc
    28741 gtcgatgatg gaagtcgtaa tgccatgttt catcaggatt ttcagggccg tgaataaaga
    28801 catatttctc tgacttcgga tgaacggtca ccacgccgac gtgtgcgccc tgtgacgcgc
    28861 gatagataac ctcgacctcg cctgtatgga tattcacacg ctcaatggtc tcgccggtaa
    28921 acgacgcccc agaaggacgc acgtcaaata ccagccactg gctgtcgggc gtccaggtat
    28981 tggtatttgt attggtattg gtattggtga gcaggtgatt acggggagca aaggttatct
    29041 gtttcatggc gcggtcctga tacaaaacgg ttcccgccat catacttcac aaggacttca
    29101 ctttcagaca atagtctttt gccatcgtga tgaatgtgag tgaaaagatg gaacatttcg
    29161 acgtggcgat tattggcctc ggcccggcag ggtcggcgtt ggcacgaaag ttagccggca
    29221 aaatgcaggt gatcgcgctg gataaaaagc accagtgcgg tactgaaggt ttcagcaaac
    29281 cttgcggcgg tctgctggca ccggacgcgc agcgatcttt tattcgcgat ggactgacgc
    29341 ttcctgtcga tgtgatcgcc aatccacaga ttttcagcgt caaaactgtc gacgtcgccg
    29401 catcgctcac gcgtaactac cagcgaagct atatcaatat taatcgccat gctttcgact
    29461 tgtggatgaa atcgctgatc cccgccagcg ttgaggttta tcacgatagc ctgtgccgaa
    29521 aaatctggcg tgaggatgat aaatggcatg tcatttttcg tgcagacggc tgggagcaac
    29581 atattaccgc ccgctatctg gtcggtgccg atggcgcaaa ctcgatggtg cggcgacatc
    29641 tctacccgga tcatcaaatc cgtaaatatg tcgctatcca gcagtggttc gcagagaaac
    29701 atccggtgcc gttctactcc tgcatctttg ataatgcgat aactgactgt tactcatgga
    29761 gtatcagcaa agatggttat tttatctttg gcggtgccta tccaatgaaa gacggtcaga
    29821 cgcgtttcac gacgctgaaa gagaaaatga gcgcctttca gttccagttt ggcaaagcgg
    29881 tgaaaagtga gaaatgcacg gtgttgtttc cctcacgctg gcaggatttt gtctgtggta
    29941 aggacaacgc ctttctgatt ggtgaagcgg cgggatttat cagcgccagc tcgctggagg
    30001 ggattagcta tgcgctggat agcgcagaga ttctgcgtgc ggtgttcctg aaacagccgg
    30061 agaagagcaa cgccgcttac tggcgcgcca cacacaaact gcgtttaaaa ctcttcggca
    30121 agatagtaaa aagccgatgc ctgaccgcac cggctttaag aaagtggatt atgcgcagtg
    30181 gtgtggcgca tattccacag ttgaaagatt atccaacgcg cttcacatca cccaccagca
    30241 ggatgtaaga gagcgcgcca atcaacgcga cggcggagat ataaaccagt gcaggcgcga
    30301 aaccgtaacc ctgcgccagg taccccacca ccagcggaac ggtgatgccg cccagaccac
    30361 cggcgaagtt aaacacgccg ccggttaaac caatcaggcg catcggtgcc agagaagaga
    30421 ccagcgacca ggtaatcgaa gcaaaaccgt tgccgaagaa tgccagcgcc atcaggcaca
    30481 taatcatcat cggatcgttg gtgtagttag cgcccataat gcaggtggag atcagcaagc
    30541 cgcagataat cggcgtttta cgcgcaaagc ccagtgaaaa gcccttacgt accagcagat
    30601 ccgcgaccca gccagagagc aggacgccga caaacgccgc gaggaatggc accgtggtca
    30661 taaagcctgc tttcagcgcc gtgattcctt tttcctgggt taaatagttc gggaaccagg
    30721 ttaagaaaaa ccacagtgca gaagccaccg caaattgccc aagataaacg ccgatcagtt
    30781 tacgatggaa caccagtttc cagtctttgg ctgttaacgg ctgacgcgcc tctttcttca
    30841 ccggcgcatc accatccacc agaccgccgc catcacgaat gtaatccagt tcagctttgc
    30901 tgataccttt ggtcaggcgc ggcggctgat aaaccttaaa ccaaatcagc gaccagataa
    30961 tgccgatacc accagtgaca atgaacaccc agtgccagct caacatctcc tgaatccaga
    31021 tcagcagcgg agtcaaaaac gccagaccga caaactgacc agacgtataa aaaccaacgg
    31081 cggaagcgcg ttcatgttcc gggaaccagc tggtcaccat ccggttattg gtcgggaaag
    31141 cgggcgcttc gaaaataccg gttatcgcac gcagaccaat taatgacatt aatcccgtgg
    31201 caaagccttg gaataaagtc gccactgacc aaccaaatat cgcaataaaa taagtcacgc
    31261 gagaacctac gcgatctaaa aaccaaccgc cggggatctg gcatagcgta taaagccagg
    31321 cgaaggccga aaatacatag cccatttccg ctttcgtaat gccgaactct tcctgaatat
    31381 gggcggaagc cacggccagg ttagcgcggt caacataaca aatgaccacc gtaataaaga
    31441 tcatcaccag cgtcagataa cgccgacgcc ccggctttgc tgcattaacg ggaatatcca
    31501 tagcgagctt tctccagatt ttgggcatag cgaagccgct caccatgccc tgtaatttac
    31561 agagggttat atttttgtat tgctgtttta gtgcccgatg aggggcttac gtggcaggaa
    31621 ttaccactct gctacgctgt tatcttcatg acgccagagc ggattacgcc agtccggggc
    31681 atttttactg aactcaataa ctttagcttc gtcgatttcc acacctaagc ccggtttcgt
    31741 taacggttta aagaagccgc caaccatgct gaagtcttct ttgtttttca caaagtcgag
    31801 taactccgcg cctttgttgt aatgaatgcc catactttgt tactgaagta ccgcgttata
    31861 ggaaacgaag tcgatatgca ggcaagccgc cagtgcaatc ggtccgaacg gacagtgcgg
    31921 cgcaagggtc acatcatagg cttctgccat tccagcaatt ttgtagcatt cggtaatacc
    31981 gcctgcatgg gagagatccg gttgcagaat cgaaataccg cctgcctcca gcacgcgttt
    32041 aaaatcgaaa cgtgagaaca tgcgctcacc cgccgccagt ggaatatgcg tttgtgccgc
    32101 caatttcggg tagtattcgg cttgttccgc cagcaccggc tcttcaataa acagcgggcg
    32161 atacggctcc agttctttaa tcagcacttt cgccattggc gcgctgacgc gaccgtggaa
    32221 atcaagacca aactcaatct gattgccaaa agcttcacga atttgtgcca cggtgttgac
    32281 tgccgcatct accgcgcggg agttatcaat tagccccagt tcttcacaac cgttcagttt
    32341 gaaggtatcg aagccgattt cgcgcagggt tttaatgccg tcgataacat ccgccggacg
    32401 atcgccgccg acccaactgt aggctttaat tttgtcgcga accaggccgc ccatcagttg
    32461 ccagactggc gcattcagca ctttgccttt gatatcccat aacgcctggt cgatcccggc
    32521 gatggcgctc atcaggattg gaccgccacg ataaaatccg gcgcgataca tcacttgcca
    32581 taagtcgttg atgcgcgaag gatcctgacc aatcaaatag tcacccagct cgtgaactgc
    32641 cgcttccacc gtacgggcgc ggccttcaat cacgggctcg ccccaaccga ccacgccttc
    32701 atcggtttca attttcagga acatccagcg gggaggtaaa cgatacgtgg taattttggt
    32761 gattttcatt gcactgcctc tcgatacgcc ttaacaaatg ctgctgcctg ctgcgcggtg
    32821 cgttctacgg actgcccggc gcgataaaga tcgctgccta agcccgcccc aacacaacct
    32881 gcgtctatcc actgcgccag gttttctggc gtcacgccgc caacggcaaa gactgcgatg
    32941 tccggtggta ataccgcttt taacgctttg atgtattgcg gaccaaaagc cgatgacgga
    33001 aatattttca gcgcctgcgc gcccgcttcg agcgcggtaa aggcttcggt cgccgtcgcg
    33061 cagccggggc aaacggtcat gccgtagccc accgcgcggc ggatcacttc actatggata
    33121 ttgggcgtaa cgatgagctg acagcccatc ctggcgagcg catcgacctg ttcaggtttc
    33181 agtaccgtac ctgcgccaat caacgccttg tcgccatacg catcaacgat ggcgggaatg
    33241 ctctgctccc attgtgggga attcagcggg atttcaaccg cgtcgaaccc ggcgtcaatc
    33301 accgcgccaa catgcgccag cgcctcgtcg ggcgtaatgc cgcgcaaaat ggcgatcagc
    33361 gggagtttag tttgccactg catgagcgat gctccttata ccagcctgaa atgccgtgtc
    33421 gcccgccacc gccgccacgt cgcaacccat cgcctgaaag gcttgctggt agcgcgcggt
    33481 cagcgatgtt ccggcgacaa gggtgatggc gtgttgatgg gtcacatagt cgcgcatact
    33541 ggcaacctct gcgccaatca acaaaccaga gagaaattcg ctgacctgtt cgcggggaag
    33601 tgttcccagc acatgcgagg cgcgaacttc aaaaagctgc ggcaatatgg cgggcgcatt
    33661 aaggccgcgc tcaaggccag ccgcgaaggc atcggcagag ttttcctgcg gcggcaaacc
    33721 tgcgccaatc aatgagtgat ttaacagtaa atgatgtaat tcaccggtca tcacggtgcg
    33781 aaaatcgttg atttgctggc tatcggcctg cacccatttg caatgcgtac cgggcataac
    33841 ataaagagag gaaggagcca gagctcgcgc gccgatcaat tgtgtttctt cgccgcgcat
    33901 cacattgtgg ttatcgtcat gagagacgca taatccggga ataatccaga tattgtcgcc
    33961 aacagacgtt aattgttcgc caatagacga aaaacgggca ggaacagata aatacggagc
    34021 aactttccag ccgacattgc tgccgaccat tcctgccatc accactggcg tattttcttc
    34081 acgccagtcg gtcgtgactt ctgctaacac tgcagccgga gattttccgt tcaggcgcgt
    34141 gacgcctgct tctgattgcc tgctctccag gcagtggtcg ccctgataaa gccaggcgcg
    34201 cagattggtc gatccccagt caattgcgat gtagcgagct gtcatgtgat ttcctttaac
    34261 cttcgtgtcg agctggcaat catggtaagc gccgcctgct ctgccgcatc gccgtcctga
    34321 tgccgtatcg catcgaacag cgccttatgt tcctggagcg tttgcggcat gttggcctca
    34381 tcgcccatcc aggttcgttc aaaaaccgcc cgctgcagcg aactgatcgc aatgctaagt
    34441 tgctgtaaca ccgggttatg caccgactgc agcaccgcct cgtggtagcg aatatccgct
    34501 tcgttaaacg cttcgcggtc ctgattgttg gcaatcatct cgttcagcgc cgattcaatc
    34561 tgcgccagat cgctggaggt cgcgcgctct gctgcccaac gggcaatcgc cggttccacc
    34621 agatttcgca cttcgctcat ggcactgata agccgtgggt cgtagtcgtt ttccagcacc
    34681 cattgcagta cgtcagtgtc gaggtaattc cactggttac gcggtgccac aaacgccccg
    34741 cgataacgtt tcatttcaat cagccgcttc gccatcagcg aacggaacac ctcacggatg
    34801 atgttgcgcg aggttgcaaa ctcttcacag agctccgcct cagccgcaag tggcgagcca
    34861 ggtacgtatt tgccgtgaac gatctgttta cccagcgtaa tgacaatgcg atcggtttta
    34921 ttgagagtca tggagagtcc ttgtgctctg tatgttcttc tctactttac cccgatcgat
    34981 gcataacgcg gcaactttgt agtaccagcg tgatgacgtt cgcgtttgcc gcgcgcgtaa
    35041 tgtagtacaa acttatattg ttgcgctaca atttagatca aaaaaagaac aacacataaa
    35101 aaaatgacat gcgtcgggca gaaatctgaa aagggatatc aggcgctaaa caggagggaa
    35161 agaagagtat gctttcaacg gcttagctac tcgtttaaag gattaatcat gaagttgaat
    35221 tttaagggat tttttaaggc tgccggttta ttcccactgg cgctgatgct ttcaggctgt
    35281 atctcgtatg ctctggtttc ccataccgca aagggtagtt caggaaagta tcaatcgcag
    35341 tcagacacca tcactgggct atcgcaggca aaagatagta atggaacaaa aggctatgtt
    35401 tttgtagggg aatcgctgga ttaccttatc actgatggtg ccgatgacat cgttaagatg
    35461 ctcaatgatc cagcacttaa ccggcacaat attcaggttg ccgatgacgc aagatttgtt
    35521 ttaaatgcgg ggaaaaagaa atttaccggc acaatatcgc tttactacta ctggaataac
    35581 gaagaagaaa aggcactggc aacgcattat ggttttgcct gtggtgttca acactgtacc
    35641 aggtcactgg aaaacctaaa aggcacaatc catgagaaaa ataaaaacat ggattactca
    35701 aaggtgatgg cgttctacca tccatttaaa gtgcgatttt atgaatacta ttcacccaga
    35761 ggcattccgg atggtgtttc cgcagcatta ctgccagtga ctgttacact ggacatcatt
    35821 actgcaccgc tgcaatttct ggttgtatat gcagtaaacc aataatcagt aagcgggcaa
    35881 acgcgtttat gctgtttgcc cgcccacaga ttaattcagc acatacttct caatagcaaa
    35941 cgccacgcca tcttcaaggt tggattttgt gacaaagttc gccacttctt tcaccgaagg
    36001 aatagcgtta tccatcgcca caccgacgcc tgcatattca atcattgcga tgtcgttttc
    36061 ctgatcgcca atcgccatga tttcttccgg tttaatacct aacacgtcgg ccagtgattt
    36121 cacacccgta cctttgttaa cgcgtttatc gaggatttcg aggaagtacg gcgcactttt
    36181 cagcacggta tatttctctt tcacttcctg cggaatacgc gcgatagcct ggtcgaggat
    36241 ggcgggttca tcaatcatca tcactttcag gaactgggta ttggggtcca ttttctccgc
    36301 ttcgcagaac accagcggaa ttgtggcaac gaaggattca tgcaccgtgt agtagctgat
    36361 atcgcggtta gcggtgtaca gcgtggtgcg gtccagggcg tggaaatgag aaccgacttc
    36421 gcgagagagt ttttccagga aacgatagtc gtcatagctg agagcggttt gcgccacggt
    36481 gctaccatca gcggccttct gtaccagcgc gccgttataa gtaatgcagt agtcgcccgg
    36541 ctgttccata tgcagctctt tcaggtagtt gtgcacacct gcatacggac gacccgtcgt
    36601 tagcacgaca ttcacgccac gggcgcgagc tgcggcaatc gcatttttaa cggcgggtga
    36661 aatggtgtga tcgggcagca gaagggtgcc atccatatcg atagcaatga gtttgatagc
    36721 catgagttcc caaggtagat tagttcctga cccatgctaa cgcgattccg ctcaaaaatc
    36781 agtacaacac ccgagggaaa aggggatgca acgcgggttg catgctcccc atttgcttag
    36841 cggaagagtt tccctttcag cagttccatg cctgcggaaa gcagatcgtt attggcttgt
    36901 gctgacactt caccttgcgg tgagagcgca tcaataatct tcggcaattg ctctgccagt
    36961 aaactggaag ccgtactggt atccacgcca agtttttgcc cgagatcgga caccgcattt
    37021 gtgccgagcg ccgactccag ttgctcgccg ctaactgatt gatttctctg ttgattactc
    37081 aaccaggttg agagaatggc ccctaagccg ccactttgca gtttttccag cagcacctga
    37141 atgccaccct gctcctcaac ccaacttaaa atcgcctgat atttccccgc atcgcctttc
    37201 agaaaggcac cgacaacttc atcaaaaagc cccatgataa tcacctgtaa agcgttacgt
    37261 gttgacccaa aaagtataga tctgcgggat gataattgcg gattgcagaa ataaaaaggg
    37321 cggagatgat ctccgccctt ttcttatagc ttcttgccgg atgcggcgtg aacgccttac
    37381 ccggcctaca aaatcatgca aaatcaatat attgcaggag tctcgtagga ctaataagcg
    37441 tagcgcatca ggcacgctcg catggttaac gccattaaat atcaatattc gccgctttca
    37501 gggcgttctc ttcaataaac gcacgacgcg gttctacggc gtcgcccatc agcgtggtga
    37561 acaactggtc agcggcaatc gcatccttaa cggtaacgcg cagcatacga cggctttccg
    37621 ggtccatggt ggtttcccac agctgttccg ggttcatctc gcccagacct ttataacgct
    37681 ggatggagag gccacgacgg gactctttca ccagccagtc cagcgcctgc tcgaagctgg
    37741 ctaccggctg acgacgctcg ccacgttcga taaacgcatc ttcttccagc aagccacgca
    37801 gtttctcacc cagcgtgcag atacgacgat attcgccacc ggtgataaac tcgtgatcca
    37861 gcggatagtc agtatccaca ccgtgggtac gcacgcgaac aatcggctcg aacaggtttt
    37921 gttcggcatt ggtgtggaca tcaaacttcc actggctgcc gtgctgttct ttgtcgttca
    37981 gttcgctgac cagcgcgttc acccagcggg taacggtctg ctcatcagaa aggtcagctt
    38041 ccgtcaacgt cggctgatag ataagctctt tcagcattgc tttcggataa cgacgctcca
    38101 tgcggttgat cattttctgc gtcgcgttgt actcagatac cagtttctct aacgcttcgc
    38161 cagccaatgc cggtgcactg gcgttggtgt gcagcgttgc gccgtccagc gcgatagaga
    38221 tctggtactg atccatcgct tcgtcgtctt taatgtactg ttcctgcttg cctttcttca
    38281 ctttgtacag cggcggctga gcgatgtaga cgtgaccgcg ttcaacgatt tccggcatct
    38341 gacgatagaa gaaggtcaac agcagcgtac gaatgtgcga gccgtcgacg tccgcatcgg
    38401 tcatgatgat gatgctgtga taacgcagtt tgtccgggtt gtactcgtca cgaccgatac
    38461 cacagccaag cgcggtgata agcgtcgcca cttcctgaga agagagcatc ttatcgaagc
    38521 gcgctttctc gacgttaagg attttaccct tcagcggcag aatcgcctgg ttcttgcggt
    38581 tacgcccctg cttcgcagag ccgcccgcgg agtccccttc caccaggtac agttcggaaa
    38641 gcgccggatc gcgttcctgg cagtctgcca gtttgcccgg caggccagct aagtcgagcg
    38701 cacctttacg gcgggtcatt tcacgcgcgc gacgcgccgc ttcacgggca cgggcagcat
    38761 cgataatttt gccaaccacg attttcgcat cagttgggtt ttccagcagg tattccgcca
    38821 gcagttcgtt catctgctgt tcaaccgccg atttcacctc agaagaaacc agtttgtctt
    38881 tggtctggga ggagaatttc gggtccggca cttttacgga aacgaccgca atcaggcctt
    38941 cacgcgcatc gtcaccggtg gcgctgactt tggctttttt gctgtagcct tctttgtcca
    39001 tgtaggcatt cagggtacgg gtcatcgccg cacggaagcc tgccaggtga gtaccgccgt
    39061 cacgctgcgg aatgttgttg gtaaagcagt agatgttttc ctggaagcca tcgttccact
    39121 gcagcgccac ttcaacgcca ataccgtctt tttcggtgga gaagtagaag atatttgggt
    39181 ggatcggcgt tttgttcttg ttcagatatt caacgaacgc tttgatgccg ccttcatagt
    39241 ggaagtggtc ttctttgccg tcacgcttgt cgcgcagacg aatggaaacg ccggagttga
    39301 ggaacgacaa ctcacgcaga cgtttcgcca gaatttcata ttcgaactcg gtcacattgg
    39361 tgaaggtttc gaggctgggc cagaaacgca ccatggtgcc ggttttttca gtctcgccgg
    39421 taaccgccag cggagcctgc ggtacaccgt gttcgtagat ctgacggtga attttaccct
    39481 cgcgctgaat aaccagctcc agtttttgcg acagggcgtt tactaccgaa acaccaacgc
    39541 cgtgcagacc gccggacact ttataggagt tatcgtcaaa tttaccgcct gcgtgcagaa
    39601 cggtcatgat cacttccgcc gccgatacgc cctcttccgg gtgaataccg gtcggaatgc
    39661 cgcgcccgtc atcctgtaca gagacagagt tatcggcgtg aatggtgacg ataatttctt
    39721 tacagtgacc cgcgagcgct tcgtcgatag cgttatctac cacctcgaat accatgtggt
    39781 gcagaccggt gccgtcatcc gtgtcgccga tatacatacc cgggcgctta cgcaccgcat
    39841 ccagcccttt caggactttg atactggagg agtcataaga attcgacatc aacgtttctc
    39901 gctcatttat acttgggtta atccgttatt ttaccctttt ccacggtaaa catcttcgaa
    39961 ttttcgtccg acatgtctat aacgtgttca gcactgatcg cgctgacaaa gacctgtgat
    40021 tgcgtcgctt ttaagcggct ggcaagcaac ccgcgacgct catcatcaag ctcagaggca
    40081 aaatcatcta tcaggtagag acaccgccgc ccgctttcac gagtgaggaa ctctccttgc
    40141 gccagacgta aggcgcacat caacagctta agctgcccac gcgataaggt atcttccacc
    40201 ggcgcaccgt cggcgcgaat gcgtaagtcc gctttatgcg ggccatgcgc ggtgtaggtt
    40261 agctggcgat cgcgttcaaa attacgttcc agcacctcag cgtactctgt ctctttctcc
    40321 cagccgcgct ggaaagagaa agtcagagaa aactcaggga gaaattgctt acaggtatca
    40381 tccatatcgg ccgcgatacc ggcgctatac tccgcgcgcc aggtgctgat ttgctccgcc
    40441 agcgggatca gttctttatc ccacgggcgt agctgttcgt aacgagtcac ctggcgcagc
    40501 gccgcattgc gctgcttgag caatcgcttg agattgctcc aggcggtgaa aaatccaggt
    40561 tcgttgtgaa agcatcccca gtcgaggaat gctcttctgt atttggggcc gccgttgagt
    40621 aaagtaaacc cttctggcgt tatcaactgc atcggcatca ggtgcgccag ttccgcgact
    40681 ttatgcccgt ccgtaccgtc aatacggact ttgctgtcgc cctgtttgtc tttggttaag
    40741 ccaatcgccg tctcgcgctc ttcgccctgt aatcgcccgt ggagaacaaa tgcctcctgc
    40801 tcatggcgaa tgacgcgacc aatctgcaaa ctgcgaaacg cccgaccatg gccgagcgta
    40861 tagatggctt ccagcacgct ggttttgcca ctgccgttgg cacctaccag aaagttaaag
    40921 ccgggagata aagcgagatc cgcggtttca atattgcgga aatcgcggat caacaagcgg
    40981 gtgagggaca ttacagtctc attggcatga caacataggc agccgactgt gatgcggcat
    41041 cttcaatctg cacgctggaa accgaatcgg tcagcatcat gcggacgttt tcgcatttca
    41101 gcgcgttcag aacatccagc acatagctga cgttgaagcc gatttccatc tccgcaccgc
    41161 tataggtaac gtcgaggatc tcttccgctt cttcctgttc cgggttgttg gcagtaattt
    41221 tcagctggtt ttcgctgaca tacaggcgca caccgcggaa tttctcgtta gagagaatcg
    41281 ccgcgcgagc aaacgcctgc ttgagcagat cgcagcctgc ttccagatgt ttgtccgggt
    41341 tcttcggcag aacgcgacgg taatccggga agcgaccatc caccagtttc gaggtgaaaa
    41401 taaagtcgcc aacgtgggca cgaatgttat tgctgccaat ctgcacacgc agcagattgt
    41461 cgccgccgtc gagcatacgc atcagttcaa tcacgccttt acgcggtacg atcaccgaat
    41521 ggcttggcaa agattgacca attggcattg aacagaccgc cagacggtgg ccgtcggttg
    41581 ccacggtgcg cagttcttca ccttcggttt caaacagcat accgtttaag taatagcgaa
    41641 catcctgatg cgccatcgaa aactgggttg cttcaatcag acgcttcatc gtcgcctgcg
    41701 gcagggtaaa ttcgacttcg ctctgccagt catcgaggtt cgggaaatcc gccgctggca
    41761 gggtagacag cgaaaaacgg ctacgcccgg agcgcaccag catccgttca ccttccagct
    41821 gcacggcaat ttccgcgcct tcaggcagac cacggcagat atcaaagaat ttgcgcgccg
    41881 gaacggtcgt cgctcctggc tcgtgtggct gaaccagcgc aacgcgtgcc accatttcca
    41941 tctcgagatc agtaccggtc agcgacaacg taccgtcagc aacctgtaac agcagattac
    42001 cgagaatcgg tagcgtagga cgaccaccta acggaccgct cacctgttgt agcggtttta
    42061 ataaatgctc acgttctacg gtaaatttca taggtttacg atgacaatgt tctgattaaa
    42121 tttgaaaaat cttctttgat atcgtggctc tcttcacgca actgctcgat cttacggcag
    42181 gcatgaagca ccgtcgtgtg gtcacgccca ccaaacgcat cgccaatctc tggcagactg
    42241 tggttagtca gctctttcgc tagcgccatc gccatctggc gcggacgcgc caccgagcgg
    42301 gatcgacgct tggaaaggag atccgcgact ttgatcttgt agtactccgc caccgtcttc
    42361 tgaatattgt cgatggtgac cagtttttcc tgcaatgcca gcaagtcgcg cagcgcctca
    42421 cgcacgaagt cgatggtgat cgcccgtccg gtaaagttgg cgttagcaat aacgcggttc
    42481 agcgcccctt ccagctcacg tacgttagat cgtagacgct tggcgataaa gaacgccact
    42541 tcacccggca aacgaatgtc attttcgtcg gcctttttca tcaggatcgc cacacgggtt
    42601 tccagctctg gcggttcgat cgccacagtc agtccccaac cgaagcggga tttcaaacga
    42661 tcctcaacgc cgttgatctc tttcggatag cgatccgagg tgagaatgat ctgttgatta
    42721 ccttccagca gggcgttgaa ggtgtggaaa aactcttcct gagatcgttc tttattagca
    42781 aaaaactgaa tatcgtcgat cagcagtgca tctacggaac ggtagtagcg tttaaactct
    42841 tcgatcgcgt tgttttgcag ggctttaacc atgtcctgaa caaagcgctc ggagtgcata
    42901 taaaccactt tggcattcgg cttgcgcgcc ataatgccgt tacccaccgc atgcagcagg
    42961 tgagttttac ccagacccgt gccgccataa aggaacaacg ggttataggc accgccaggg
    43021 ttatccgcca cctggcaagc cgccgcgcgc gccagttggt tagatttacc ttcaacgaag
    43081 ttatcaaacg tgtgtttgac gtttacgtta gaacgatagg tcggttctgc cggggccggg
    43141 acgttatccc aacctgagcg cgtagaaggc gcagcacgtt gcggctgcgt ttgcgccacc
    43201 tgtgcagggg ccgcgacgtt gctcgtcact gccgcttgtg gcgtttgcgt caccggtttg
    43261 gtgccgactt caaaacgcag ctgtggggca tccgctccgc agaaactggt tagcagtcca
    43321 ttgatattat taaggtactt gtcccgtacc caatcgagga caaaacggtt tggcgcgtac
    43381 agggccagcg tgttatcgct cagttccgcc tgcaatgggc gtatccacat actgaattct
    43441 gtggctggta actcatcctg caatcgggca agacactgct gccaaagcga aagtgacacg
    43501 gcggactcca ctcgaacaaa agtcgataat gactaaggct gaaacattca tgattgttga
    43561 cgtacgtcga aaagaccctg cttgagggtg acgcacgaac cgctgtctgc ggttatatgc
    43621 ccgatcaaga tcctgcaaaa cgatcgggac cgcggatcat agcctaaact gcgcaagaga
    43681 tcttctgttt ctcacagatt ttccctattt atccacagga ctttccagaa ctcgctaagt
    43741 gtaaacgatc ctgccgcgag gcgggcacga tttacgccgc atattggaaa atttaatgac
    43801 catagacaaa aattggctta atcgatctaa taaagatcca ggacgatcct tgcgctttac
    43861 ccatcagccc gtataatcct ccacccggcg cgccatgctg gtttccactg gtgtgaggtc
    43921 gtacattttc cctgcgaaaa ggtgcggaaa agcgcggtaa ataaggaaag agaattgact
    43981 ccggtgtgta caattattac aatccggcct ctttaatcac ccatggcttc ggtgtccatc
    44041 gtttcatttt tcggcggata tccaataaag ccattgaatt tattcaagtt taggtagaaa
    44101 tcgccatgaa acgcactttt caaccgtctg tactgaagcg caaccgttct cacggcttcc
    44161 gtgctcgtat ggctactaaa aatggtcgtc aggttctggc acgtcgtcgt gctaaaggcc
    44221 gcgctcgtct gaccgtttct aagtaataaa gctaacccct gagtggttaa gctcgcattt
    44281 cccagggagt tacgcttgtt aactcccagt caattcacat tcgtcttcca gcagccacaa
    44341 cgggctggca cgccgcaaat taccattctc ggccgcctga attcgctggg gcatccccgt
    44401 atcggtctta cagtcgccaa gaaaaacgtt cgacgcgccc atgaacgcaa tcggattaaa
    44461 cgcctgacgc gtgaaagctt ccgtctgcgc caacatgaac tcccggctat ggatttcgtg
    44521 gtggtggcga aaaaaggggt tgccgacctc gataaccgtg ctctctcgga agcgttggaa
    44581 aaattatggc gccgccactg tcgcctggct cgcgggtcct gatagccctc attcgggtct
    44641 atcaacgcct gattagtccg ctactcgggc cgcattgtcg tttcactcca acctgttcaa
    44701 gctacggaat tgaggcattg cgcaggtttg gagtgataaa aggcagttgg ttgacggtga
    44761 aacgcgtatt aaaatgccac cctttacacc ctggtggtga cgatcccgtc ccgcccggac
    44821 catttgatac cagagaacac taacgatgga ttcgcaacgc aatcttttag tcatcgcttt
    44881 gctgttcgtg tctttcatga tctggcaagc ctgggagcag gataaaaacc cgcaacctca
    44941 ggcccaacag accacgcaga caacgaccac cgcagcgggt agcgccgccg accagggcgt
    45001 accggccagt ggccagggga aactgatctc ggttaagacc gacgtgcttg atctgaccat
    45061 caacacccgt ggtggtgatg ttgagcaagc tctgctgcct gcttacccga aagagctgaa
    45121 ctctacccag ccgttccagc tgttggaaac ttcaccgcag tttatttatc aggcacagag
    45181 cggtctgacc ggtcgtgatg gcccggataa cccggctaac ggcccgcgtc cgctgtataa
    45241 cgttgaaaaa gacgcttatg tgctggctga aggtcaaaac gaactgcagg tgccgatgac
    45301 gtataccgac gcggcaggca acacgtttac caaaacgttt gtcctgaaac gtggtgatta
    45361 cgctgtcaac gtcaactaca acgtgcagaa cgctggcgag aaaccgctgg aaatctcctc
    45421 gtttggtcag ttgaagcaat ccatcactct gccaccgcat ctcgataccg gaagcagcaa
    45481 cttcgcactg cacaccttcc gtggcgcggc gtactccacg cctgacgaga agtatgagaa
    45541 atacaagttc gataccattg ccgataacga aaacctgaac atctcttcga aaggtggttg
    45601 ggtggcgatg ctgcaacagt atttcgcgac ggcgtggatc ccgcataacg acggtaccaa
    45661 caacttctat accgctaatc tgggtaacgg catcgtcgct atcggctata aatctcagcc
    45721 ggtactggtt cagcctggtc agactggcgc gatgaacagc accctgtggg ttggcccgga
    45781 aatccaggac aaaatggcag ctgttgctcc gcacctggat ctgaccgttg attacggttg
    45841 gttgtggttc atctctcagc cgctgttcaa actgctgaaa tggatccata gctttgtggg
    45901 taactggggc ttctccatta tcatcatcac ctttatcgtt cgtggcatca tgtacccgct
    45961 gaccaaagcg cagtacacct ccatggcgaa gatgcgtatg ctgcagccga agattcaggc
    46021 aatgcgtgag cgtctgggcg atgacaaaca gcgtatcagt caggaaatga tggcgctgta
    46081 caaagctgag aaggttaacc cgctgggcgg ctgcttcccg ctgctgatcc agatgccaat
    46141 cttcctggcg ttgtactaca tgctgatggg ttccgttgaa ctgcgtcagg caccgtttgc
    46201 actgtggatc cacgacctgt cggcacagga cccgtactac atcctgccga tcctgatggg
    46261 cgtaacgatg ttcttcattc agaagatgtc gccgaccact gtgaccgacc cgatgcagca
    46321 gaagatcatg acctttatgc cggtcatctt caccgtgttc ttcctgtggt tcccgtcagg
    46381 tctggtactg tactatatcg tcagcaacct ggtaaccatt attcagcagc agctgattta
    46441 ccgtggtctg gaaaaacgtg gcctgcatag ccgcgagaag aaaaaatcct gattcggtga
    46501 gttttcgcta aaataagggc ggtcagttga ccgccttttt tcttttcgta gggcggataa
    46561 gcaccgcgca tccgccacac aaagcaacag gaacatcatg agcgataatg acactatcgt
    46621 agcccaggcc acgcctccgg gacgtggcgg cgttggcatc ctgcgcatct ccggcttcaa
    46681 agcccgtgaa gttgccgaaa ccgtgctggg taaactgcct aagccgcgct acgccgatta
    46741 tcttccgttt aaagacgccg acggcagcgt gctcgatcag gggattgcgc tatggttccc
    46801 tggcccgaac tcgttcaccg gcgaagatgt gctggaactg caaggtcatg gcggtccggt
    46861 gatcctcgac ctgctgttaa aacgcattct gaccattccc ggcctgcgga ttgctcgccc
    46921 tggtgagttt tccgaacgcg cgtttcttaa cgataaactt gacttagccc aggccgaggc
    46981 gattgccgat cttatcgacg ccagttcgga acaggcggcc cgttcggcac ttaactcgct
    47041 gcaaggcgca ttctccgcac gggttaatca tctggtagaa gccctcaccc acttgcgcat
    47101 ttacgtcgaa gcggcaattg atttccctga tgaagagatc gatttcctct ccgacggcaa
    47161 aattgaagcc cagcttaatg acgtgatggc cgatctcgat gcagtgcgtg ctgaagcacg
    47221 tcagggtagt ttgttgcgcg aagggatgaa agtagtgatt gccggacgtc ctaatgccgg
    47281 taaatcgagc ctgttaaacg cgctggcggg ccgtgaagcg gcaatcgtaa ccgatatcgc
    47341 cggaaccacg cgtgacgtgc tgcgtgagca tatccacatt gacggaatgc cgctgcatat
    47401 catcgacact gccgggctac gtgaggccag tgacgaagtg gaacgtattg gtatcgagcg
    47461 cgcgtggcag gaaattgaac aggccgaccg cgtgctgttt atggtcgatg gcaccacgac
    47521 agacgccgtt gatccggcag agatctggcc ggaatttatc gcccgtctgc ccgcgaaact
    47581 gccgatcacc gtggtacgca ataaagccga tatcaccggc gaaacgctgg gaatgagtga
    47641 agtgaacggt cacgcgttaa ttcgtctctc ggcaaggact ggtgaaggcg tggacgtgct
    47701 gcgtaaccat ctcaaacaga gcatgggctt tgacaccaat atggaaggcg gcttcctggc
    47761 gcgccgtcgc cacttacagg cgctggaaca ggcagcggag catttgcagc aaggtaaagc
    47821 gcaactgttg ggagcctggg caggtgaact gctggcggaa gagttgcgcc tggcacagca
    47881 gaacttaagc gaaatcaccg gggaatttac ttcagacgac ctgctggggc ggattttctc
    47941 cagcttctgt attggtaagt aaccgcgctt acgaagccgt attctgactg tcagatgcgg
    48001 cttcgcttca ttgttagcac tcctgttatg cctcaaccct tttttaaaca ttaaaattct
    48061 tacgtaattt ataatcttta aaaaaagcat ttaatactgc tccccgaacg
//
LOCUS       DAJTQQ010000023        47606 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000023 DAJTQQ010000000
VERSION     DAJTQQ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.023"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO309_001422"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO309_001422"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR5846611.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="OO309_001423"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="OO309_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR5846612.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO309_001424"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO309_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR5846613.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO309_001425"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO309_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR5846614.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO309_001426"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO309_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR5846615.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="OO309_001427"
     CDS             5377..5835
                     /locus_tag="OO309_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR5846616.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="OO309_001428"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="OO309_001428"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR5846617.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="OO309_001429"
     tRNA            6887..6971
                     /locus_tag="OO309_001429"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="OO309_001430"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="OO309_001430"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO309_001431"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO309_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR5846618.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO309_001432"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO309_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR5846619.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO309_001433"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO309_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5846620.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="OO309_001434"
     CDS             complement(10252..13995)
                     /locus_tag="OO309_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR5846621.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO309_001435"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO309_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR5846622.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="OO309_001436"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="OO309_001436"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR5846623.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="OO309_001437"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="OO309_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR5846624.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO309_001438"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO309_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR5846625.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="OO309_001439"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="OO309_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR5846626.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO309_001440"
     CDS             complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO309_001440"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR5846627.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO309_001441"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO309_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR5846628.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21887..22987
                     /gene="chaA"
                     /locus_tag="OO309_001442"
     CDS             21887..22987
                     /gene="chaA"
                     /locus_tag="OO309_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR5846629.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23392..23499
                     /locus_tag="OO309_001443"
     CDS             23392..23499
                     /locus_tag="OO309_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5846630.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO309_001444"
     CDS             complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO309_001444"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR5846631.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24538..25347)
                     /locus_tag="OO309_001445"
     CDS             complement(24538..25347)
                     /locus_tag="OO309_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR5846632.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25351..25743)
                     /locus_tag="OO309_001446"
     CDS             complement(25351..25743)
                     /locus_tag="OO309_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR5846633.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO309_001447"
     CDS             complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO309_001447"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5846634.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO309_001448"
     CDS             complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO309_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR5846635.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO309_001449"
     CDS             complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO309_001449"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR5846636.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29167..29790
                     /gene="lolB"
                     /locus_tag="OO309_001450"
     CDS             29167..29790
                     /gene="lolB"
                     /locus_tag="OO309_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR5846637.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29790..30641
                     /gene="ispE"
                     /locus_tag="OO309_001451"
     CDS             29790..30641
                     /gene="ispE"
                     /locus_tag="OO309_001451"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR5846638.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30792..31739
                     /gene="prs"
                     /locus_tag="OO309_001452"
     CDS             30792..31739
                     /gene="prs"
                     /locus_tag="OO309_001452"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR5846639.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31864..33543
                     /gene="dauA"
                     /locus_tag="OO309_001453"
     CDS             31864..33543
                     /gene="dauA"
                     /locus_tag="OO309_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR5846640.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO309_001454"
     CDS             complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO309_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR5846641.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34154..34738
                     /gene="pth"
                     /locus_tag="OO309_001455"
     CDS             34154..34738
                     /gene="pth"
                     /locus_tag="OO309_001455"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5846642.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34855..35946
                     /gene="ychF"
                     /locus_tag="OO309_001456"
     CDS             34855..35946
                     /gene="ychF"
                     /locus_tag="OO309_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR5846643.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36790..39657
                     /locus_tag="OO309_001457"
     CDS             36790..39657
                     /locus_tag="OO309_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5846644.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLSAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="OO309_001458"
     CDS             complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="OO309_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR5846645.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41904..42974
                     /gene="dhaK"
                     /locus_tag="OO309_001459"
     CDS             41904..42974
                     /gene="dhaK"
                     /locus_tag="OO309_001459"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR5846646.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDAMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42985..43617
                     /gene="dhaL"
                     /locus_tag="OO309_001460"
     CDS             42985..43617
                     /gene="dhaL"
                     /locus_tag="OO309_001460"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR5846647.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43628..45046
                     /gene="dhaM"
                     /locus_tag="OO309_001461"
     CDS             43628..45046
                     /gene="dhaM"
                     /locus_tag="OO309_001461"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR5846648.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45366..47063
                     /gene="treA"
                     /locus_tag="OO309_001462"
     CDS             45366..47063
                     /gene="treA"
                     /locus_tag="OO309_001462"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5846649.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(47142..47581)
                     /locus_tag="OO309_001463"
                     /pseudo
     CDS             complement(47142..47581)
                     /locus_tag="OO309_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        11953 a        12273 c        11655 g        11725 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgctgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct atagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggc ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgacca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgtggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatagt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt taccgctcat
    21301 tttgaaacca ctaacggaac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    21781 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    21841 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    21901 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggtgctggt
    21961 agtgctgttc ctgtggggaa gtagccagac actaccagtg gtcattgcca tcaatcttct
    22021 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    22081 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    22141 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    22201 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    22261 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    22321 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    22381 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    22441 tttgctgatc caggccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    22501 tgatgacgac gacccgcacc acggtaaaac gtctgcccat agcagcctgt ggcatgctat
    22561 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagctcgct
    22621 ggagacattg ctcgacagca tgaatgcccc tgttgccttt actggcttcc tggtggcact
    22681 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggttca
    22741 gcgcgcgatg aatctgttct ttggttcagt gttagcaaca atttcgctta ccgtacctgt
    22801 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    22861 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    22921 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    22981 tgcctgacag ttatatctcc tccgatgacg gaaatcggga gagataagat aaattcctgc
    23041 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    23101 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    23161 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    23221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    23281 aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc
    23341 agacacttca agaatagccg caaaatgttg tcattacaac aaggcggcta tatgacgctc
    23401 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
    23461 gccgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
    23521 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc ggcctttttt
    23581 gtatctggca cccatgaaaa aagtcttaac gcagaacgct aagactttat ttttcaagca
    23641 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    23701 tcatctgctt gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    23761 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    23821 cggctcgtgc cagctcagcc acctgagcac gacgaccacc ggaagctgcg ccaaacggat
    23881 cgcggcattg cagtgcgtgg gtcacgtcga aaatcaccgg cgagttacca gacactttct
    23941 tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag ttagcaccac
    24001 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    24061 tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg gttttcgcca
    24121 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    24181 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    24241 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    24301 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    24361 gtttctgggt cacagttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    24421 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    24481 tgctaaccac tttttgtttc ataggatcgc cttaatcttg agtaaaatgt cgattaatta
    24541 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    24601 cgggtcttcc ggacaccgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    24661 atccagttgc gcataaatca acccacgatc gcgaatttca tagggatctt cagggttgaa
    24721 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    24781 ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    24841 ttcataaaac agttccgccg acgggctgat atttcccttt aaccatccgt ccagcatatg
    24901 ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat ccggacattc
    24961 aatgcgcaat atcagctgcg tagggaaaat caccggcagc agcggcaaat cgagacgatt
    25021 cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat tctttaacac
    25081 ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt taaatcccca
    25141 gtcgccgtaa aacagcgcga tcagtttttc cagctgctct tctaaaggca gaagctggct
    25201 gatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat cttgcgaggg
    25261 aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg gcgctttatt
    25321 aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt ttgtggtggc
    25381 gagtttaatg atgatgtaca gcaccaccaa cgccagcggg aaggcaataa tgcgcgcctg
    25441 ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacgatat aaatgataac
    25501 tccaaacagc ttttcagtca gccactgtgc ctgcccggaa aatggcagga tgtgcgcttt
    25561 agccatcaac gcaatgccgc ttaacaataa caccgtatcg acaaccggcg gcacaatgcg
    25621 cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac gtaaggttaa
    25681 tagcccaaca gaaagcgcga tactaataag atgaacacta agcagtgtag aaaaacttgt
    25741 cattgataat agcggccgag cgttacgcgc tcgttatcac catagtcacg gcaggtttcg
    25801 acgtcatgat accccgcgag gataaatgct tgtcgcaccg cttcgccctg ctgccagcca
    25861 tgttccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc gatgatatgc
    25921 acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc aaagcggaca
    25981 tcgccttgtt gaagatgtgg gtcctgctcg tcaatatacg gcggattgct gacaatcatc
    26041 gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg
    26101 atcgccagat gctgggcatt acgttgtgcc agggagacag catcaggcat acgatctaca
    26161 gcgataattt cgcagtccgg gcgctcgcta gccagcgcaa gcgcaatcgc cccggtaccc
    26221 gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc
    26281 agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa cggcaacgac
    26341 cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg acgtgtcagt
    26401 agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata
    26461 aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg ccgcgggctt
    26521 tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc cattattcct
    26581 gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt tcaatcagca
    26641 tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg atgcggtgat
    26701 cggtaacgcg cccctgcggg aaattgtaag tacggttacg gtcgctgcga tcgccactcc
    26761 ccagcaggtt acgacgggta gacgcttcgg cctgttggcg ttttgccatt tcagcagcgt
    26821 ggatgcgagc accgagcact gaaagcgcct tcgctttgtt tttatgttgt gaacgttcgt
    26881 cctgacattc aacaacaatc ccggtcggca agtgagtaat acgaattgcc gaatcggtgg
    26941 tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt atcaatgcgt aaatctgctg
    27001 ggttgatgtc cggcagttct gcgtcaggca gttctggcat taccgcaacg gtacaagcag
    27061 aagtatgaat acgaccctgc gattccgtag caggaacacg ttgcacgcga tgaccgccgg
    27121 attcaaattt cagacgacca tacacaccat caccgctaat tttggcgatg atctctttat
    27181 aaccaccatg ttcaccctcg ctggcgctca tgatttctac ccgccagcgg cgggcttcgg
    27241 cgtaacggct gtacatacgg aacagatcgc ctgcaaacag cgctgcttcg tcgccgccgg
    27301 ttccggcacg gacttcgagg aaggcgttac gttcgtcatc aggatctttt ggtagtaata
    27361 gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt agcttcgcgc agttcatcct
    27421 gcgccatctc acgcatttca ggatcgtcga gcatcatctg tgcggtttcg atatcttcct
    27481 gaacctgttg ccagtcggta aaacagcgcg aaacatcact taactgcgca tattcgcgtg
    27541 ataatgcgcg aaaacgttcc tggtcggcga tagtttgcgc gtcacccagc aacgcctgaa
    27601 cttcttcatg gcgttcatgc agggcttcca gtttggcaac gatagaaggc ttcataggcg
    27661 taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac tccagcccga ggctgtcgcg
    27721 cagaatattc aggcgttcgt tatccccgtc acgggcggcc tgttgaagtg atttcgttgg
    27781 cgcatggatc aagcggttag tcagtttcca tgccagatcc tgcataatgg cttgcgcgtc
    27841 gccgccctgc tcaagggccg ctaacgcttt ggcggttaac tcatcgcgaa cgtgctctgc
    27901 ctggctgcga tactcgcgaa tggtttcgct ggcgctttgt gctcgcagcc acgccataaa
    27961 ttcgctggtt tcctgagcga caatagtttc cgcctcaacc gctgcggctt tacgctgcgc
    28021 caggttgtgc gaaatgatgc tttgcagatc gtccacacta taaagataag cattcgccag
    28081 tttgccaact tccggctcaa catcgcgcgg aacggcaata tccaccaaca gcattggttg
    28141 gttacgacga ctttttaatg cgcgctccac catgcctttc ccgataatcg gtaacgggct
    28201 ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt tcgtcgatct cactcagggc
    28261 aatcacttcc gcgccaactt catctgccag aatttgggca cgttcgcgag tgcggttggc
    28321 gataatcatc ttctgtactt tatgttcgcg cagatgacgc gctaccagct cgatggtttc
    28381 gcccgcgcct accagcaaca ctgtgaccgt agagagcgat tcaaagatct gccgcgccag
    28441 cgtacaagcc gcaaaagcga cagacacagc actggcaccg atatctgttt cagtgcgaac
    28501 gcgtttcgct acagagaaag atttctggaa catgcgttcc agttcgctgg ccttcatatg
    28561 acctttttgc gaatcgacaa acgctttttt aacctgaccg aggatctgcg gctccccaag
    28621 aaccagtgaa tccaggccgc tggcaacacg cattaaatgg ctaaccgcgt cgttatcctg
    28681 atgccagtag aggcttttac gcagatcttc ttcattaaga ttgtgataat cgcaaagcca
    28741 gcggattaac gcctcttgca ggttatcctg ctcttcaacg ctaagataaa gttccgtgcg
    28801 gttgcacgtc gacagcacca cgccgccctg caccatcggc tgcgcaagca ggctgtcaag
    28861 cgcctgatcg agcttatccg gcgaaaacga tacacgttct cgcagcgata caggtgccgt
    28921 tttatggttg ataccgagtg ctaaaagggt catgtctgcg ggaaataata ccaacgttga
    28981 tagggttagt ctgcttgcat catacaggat gcgtaagatc aataaaagag agcgccccct
    29041 tttggagtaa ttgccgaaag ccgttagatt ctggcaatta agacaacttg aacatagacg
    29101 atagcggacg gtaacgctag cattaagggt tataactgca acgtatctca aggacttgtc
    29161 atcactatgc ccctgcccga ttttcgtctt atccgcctgc taccgctggc tgctcttgtg
    29221 ctcactgcct gttccgttac cacgcccaaa ggtcctggca aaagcccgga ttcgccacaa
    29281 tggcgtcagc atcagcaaga cgtgcgcaat cttaatcagt atcagactcg cggcgcgttc
    29341 gcttatattt ctgaccaaca aaaagtgtac gcccgctttt tctggcagca aaccggccag
    29401 gatcgctacc gtctgctgct cactaaccca ttgggcagca cggaactgga gctgaatgct
    29461 caaccgggta acgtgcagtt agtcgacaat aaaggtcagc gttataccgc cgatgacgcc
    29521 gaagagatga ttggcaaatt gaccggaatg ccaattccgc tcaacagctt gcgccagtgg
    29581 attttaggtt taccgggtga tgcaaccgac tacaaactgg acgaccagta ccgcctgagc
    29641 gaaattacct acagccagaa tggcaaaaac tggaaggttg tttatggtgg ttatgacacc
    29701 aaaacgcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca acgcatcaag
    29761 ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct ccggcaaaac
    29821 ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc
    29881 tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac gatggggata
    29941 ttcgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag
    30001 cgcggttgtt gatgaaaact gcggcagaca gcgggcgtct tccgacggga agcggtgcga
    30061 atatcagcat tgacaagcgt ttgccgatgg gcggcggtct cggcggtggt tcatccaatg
    30121 ccgcgacggt cctggtggca ttaaatcatc tctggcaatg cgggctaagc atggatgagc
    30181 tggcggaaat ggggctgacg ctgggcgcag atgttcctgt ctttgttcgg gggcatgccg
    30241 cgtttgccga aggcgttggt gaaatactaa cgccggtgga tccgccagag aagtggtatc
    30301 tggtggcgca ccctggtgta agtattccga ctccggtgat ttttaaagat cctgaactcc
    30361 cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa atgtgaattc agcaatgatt
    30421 gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc ggtgctttcc tggctgttag
    30481 aatacgcccc gtcacgcctg actgggacag gggcctgtgt ctttgctgaa tttgatacag
    30541 agtctgaagc ccgccaggtg ctagagcaag ccccggaatg gctcaatggc tttgtggcga
    30601 aaggcgttaa tctttcccca ttgcacagag ccatgcttta agccgggcaa gctgagtttc
    30661 ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc tttaatacac cgcctggaaa
    30721 ggatcatgcc tggcccgcac agttttcggc agattctttc caccaatgga cgcatgcctg
    30781 aggttcttct cgtgcctgat atgaagcttt ttgctggtaa cgccaccccg gtactagcac
    30841 aacgtattgc caaccgcctg tacacttcac tcggcgacgc cgctgtaggt cgctttagcg
    30901 acggcgaagt cagcgtacaa attaacgaaa atgtacgcgg tggtgatatt ttcatcatcc
    30961 agtccacttg tgcccctact aacgacaacc tgatggaatt agtcgttatg gttgatgccc
    31021 tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc ctactttggc tatgcgcgcc
    31081 aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc gaaagtggtt gcagacttcc
    31141 tctccagcgt cggtgttgac cgtgtgctga cagtggatct gcacgctgaa cagattcagg
    31201 gtttcttcga cgttccggtt gataacgtat ttggtagccc gatcctgctg gaagacatgc
    31261 tgcagctgaa tctggataac ccaattgtgg tttctccgga catcggcggc gttgtgcgtg
    31321 cccgcgctat cgctaagctg ctgaacgata ccgatatggc aatcatcgac aaacgtcgtc
    31381 cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga cgttgcaggt cgtgactgcg
    31441 tactggtcga tgatatgatc gacactggcg gtacgctgtg taaagctgct gaagcgctga
    31501 aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca cccgatcttc tctggcaacg
    31561 cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt tgtctgcgat accattccgc
    31621 tgagcgatga aatcaaatca ctgccgaacg tgcgtactct gaccctgtca ggtatgctgg
    31681 ccgaagcgat tcgtcgtatc agcaacgaag aatcgatctc tgccatgttc gaacactaat
    31741 cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc catttgtatg
    31801 acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg atgaaacatt
    31861 attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat cgacgtttgc
    31921 tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg gataaccgtc
    31981 gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt ggccccccag
    32041 tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctaacggg tgggtcacgc
    32101 tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt ttcgcaacag
    32161 tttggactgg caggactgct ggttgcgacc ttgttgtcgg gaatcttttt gattctgatg
    32221 gggctggcac gctttggtcg tctgattgag tatattccgg tttccgtcac cttaggtttc
    32281 acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct cggtctgcaa
    32341 atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat ggcgctgccg
    32401 accattaatg tgggtgatgc tgccattggc attgtgacgc ttggcattct ggttttctgg
    32461 ccgcgtctgg gcattcgttt acccggtcac cttccggcct tgctggctgg ttgcgcggtg
    32521 atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca attccactac
    32581 gtcctggccg atggttctca gggtaacggt attccgcaac tgctgccgca actggtgctg
    32641 ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg caccctgctg
    32701 cctgcggcat tctcaatggc aatgctcggg gcgatcgaat ctctgctctg cgctgtggta
    32761 ctggatggta tgaccgggac taagcacaag gcgaatagtg aactggtcgg tcaggggctg
    32821 gggaatatca tcgctccatt ctttggtggt attaccgcta ctgctgccat cgcgcgttct
    32881 gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca ctctattctg
    32941 gttattctcg ccctgctggt actggcacca ctgctctcct ggctgccgct ttccgccatg
    33001 gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcacaaagt ggtcgacttg
    33061 ctgcgtcatg cgccgaaaga tgacatcatc gtcatgctgc tgtgtatgtc actgaccgtg
    33121 ctgtttgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct gctgtttatg
    33181 cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagatgttcc agacgatgtc
    33241 ctggttctgc gcgttattgg cccgctgttt tttgctgctg ctgaaggctt attcacggac
    33301 ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc cgttccggta
    33361 cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc cgaaggatgt
    33421 gaactgcgcg tgtgcaacgt ggaattccag ccactgcgaa ctatggctcg cgcaggcatt
    33481 caaccgatcc cgggacgcct cgcgttcttc ccgaatcgtc gcgcggcgat ggcggattta
    33541 taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca ttatttgtta
    33601 gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtgacgaa cccaccagta
    33661 acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga gaatggcacc
    33721 gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa actgtggtaa
    33781 ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca accccatgag
    33841 cacgttaccg agtatcgaag cgtttttgcg tttcatatgt cacctccgga actttctggg
    33901 ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc ctgtgggtta
    33961 tgtgcgggcg taatcacaat tacaaccctt atttcaacaa aactttacaa ataaacgcct
    34021 gaacaccttg actttctaat agtccactca ggcaattatt acgttatctg catttgatac
    34081 gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat gtacacactc
    34141 aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata
    34201 cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag agcgtttgcg
    34261 cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca cccttggaag
    34321 cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca aagccgttgc
    34381 ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtggccc acgacgaact
    34441 ggatctgcct cctggcgtcg ccaaatttaa gttgggcggt ggccatggtg gtcacaatgg
    34501 actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt tacgcatcgg
    34561 aatcggtcat ccgggcgata aaaataaagt tgtcggtttt gtgttaggca aaccgcctgt
    34621 tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta ctgaaatgtg
    34681 gtttacagat ggcttgacca aagcaacgaa ccgattgcac gcctttaaag cgcaataagt
    34741 cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat ttccatttct
    34801 gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta aatcatggga
    34861 ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt caacgcgctg
    34921 accaaagccg gtattgaagc ggccaacttt ccattctgca ccattgagcc gaacacaggc
    34981 gtcgtaccaa tgcctgaccc tcgcctggat caactggctg aaatcgtaaa accgcaacgt
    35041 acgcttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg cgcatcgaaa
    35101 ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc gatcggtcac
    35161 gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt taacccggct
    35221 gacgatattg aagttatcaa caccgaactg gcgctggcag acctcgacac ctgcgaacgt
    35281 gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa agctgagctg
    35341 gcggtcctgg aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg cgcgctggat
    35401 ttaagcgctg aagagaaagc ggctattcgt tacctgagct tcctgacgct gaaaccaaca
    35461 atgtacatag ccaacgtcaa cgaagatggt tttgaaaaca acccatatct tgaccaggtg
    35521 cgtgaaatcg cggcgaaaga aggttctgtt gtggttccgg tttgtgctgc tgttgaagca
    35581 gacattgccg aactggacga cgaagaacgt gacgagttta tgcaggagct tgggctggaa
    35641 gaaccgggcc tgaaccgtgt gatccgtgcc ggttataagc tgctgaacct gcaaacttac
    35701 ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggagc aaccgcgccg
    35761 caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc acaaaccatc
    35821 tcgtttgaag atttcatcac ttacaaaggt gaacaaggcg cgaaagaagc aggcaaaatg
    35881 cgtgcagaag gtaaagatta catcgtgaaa gatggcgatg tgatgaactt ccttttcaac
    35941 gtctaattaa tttattgtct catgaggttt cgctaaatct catgaagagc gcaaaagtat
    36001 ataaaatcca cgcaattgcg tggatttttc atttcaccga gtcctaatca ttcttgtaac
    36061 atcctacact tttgtgctta ttgcctacag tgaaccacga gaataattaa agacataagt
    36121 gttcagatga ctgatatctc attagcagtg atataacgtc agtttttgtt ccactacaca
    36181 tcagccctct tcacagctga tcaatattga tctcgctcca atatgctggc gagaaaagga
    36241 cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt acgtgcgatc
    36301 ctggcaagat gaaaatgata accagtagca caataataac gataggccag aaaatccatt
    36361 gaacaaaaat gaatatcgat gttttttgct ttttcttatt agtaatgcaa aaatggcgca
    36421 tattgttttt gttatgcaga tacatcaagc gaaaatccca taactaatta ggattattac
    36481 aaaaaatctt caccaaattt tttaaccgct gtttgttgta atatttttgc catattcata
    36541 gtaacgaatt ggaataagta acgacaactt agggaataac tcgatataaa ctcacctttg
    36601 taaggggggg gttaaatata tattaattcc tgtgttcctg attttgcccg gtaatagaga
    36661 attattctca gagaagctca tcatgattgc accatacaga tatcatgatg ataagaggat
    36721 attattcatt gacttaaaca aaatgacaaa atccatagac ccatagcctg ggtactatat
    36781 aggagtggta tgggcatcaa acaacacaat gggaatacca aagccgatcg tctcgctgaa
    36841 ttaaaaatcc gttcgccctc aattcaactg ataaaatttg gcgctattgg tttgagtgca
    36901 attatctttt cccccctgct gatagctgct gatacaggaa gtcaatatgg caccaatatt
    36961 actattaatg atggtgacag aattacagga gataccgccg atccatcagg aaacctctat
    37021 ggtgtaatga ccccagcagg aaacacgcct ggcaatatca acctgggtaa tgatgtcacc
    37081 gtcaatgtca acgacgcctc tggatatgca aaaggaatca ttattcaggg caaaaacagc
    37141 tccctgacag ctaaccgact cacagtagat gttgttggtc aaacctctgc catcggcatt
    37201 aacttaattg gtgactatac ccatgctgac ttaggcacag gcagcaccat taagagtaac
    37261 gatgacggca tcattattgg gcatagctca acactaacag ccactcaatt caccattgaa
    37321 aactcgaacg gtataggcct aaccatcaat gactatggca ccagtgtcga tcttggaagc
    37381 ggaagtaaaa tcaagaccga tggaagtaca ggtgtttata tcggtggtct caacggcaat
    37441 aacgccaatg gtgctgcgcg ttttacggcg acagacctga caatcgatgt tcagggctac
    37501 agcgccatgg ggataaacgt acagaaaaac tctgttgtcg atctcggaac aaacagtacc
    37561 attaaaacca atggcgataa tgctcacggc ctctggagct ttggccaggt tagcgcgaat
    37621 gcactcactg ttgatgtaac tggagccgcg gccaatggcg tcgaagttcg tggtggtaca
    37681 accactatcg gtgcagatag ccatatttct tccgcgcagg gcggtggcct cgtcaccagt
    37741 agttcagacg cgacaatcaa tttttctggc acggcagcgc aacgaaacag catcttttcc
    37801 ggcggttctt atggtgcctc ggcccagacg gcaacggctg ttatcaacat gcaaaatacc
    37861 gatattacgg ttgatcgtaa tggcagtctg gcgctgggtt tgtgggcgct cagcggcggt
    37921 agaataaccg gagacagttt ggctatcacc ggcgcggcag gagccagagg gatttatgcc
    37981 atgaccaaca gccagatcga cctcacgagc gatctggtca ttgatatgag tacacccgac
    38041 cagatggcca tcgcaacgca acatgacgat ggttatgccg ccagccgcat caacgcctcg
    38101 ggtcgtatgc ttatcaacgg tagcgttctt tccaaaggtg ggctaatcaa tctggatatg
    38161 caccctgggt cggtttggac aggttcctcc ctcagcgata atgtcaatgg cgggaaactg
    38221 gacgttgcaa tgaataacag cgtctggaac gtaacaagta attctaatct cgacacgctg
    38281 gcgctgagcc attcaactgt cgattttgcc agccacgggt caactgccgg cacatttacc
    38341 acattaaacg tagagaacct gagcggtaac agtaccttta ttatgcgtgc tgatgttgtt
    38401 ggcgagggta atggcgttaa taatagaggg gatttattga atatcagcgg gagtagtgct
    38461 ggtaatcacg tattggctat ccgcaaccag ggcagcgagg ccacaacggg aaatgaagtt
    38521 ctgacagtgg taaaaaccac tgacggcgcg gcctcgttca gcgcgtcttc tcaggttgag
    38581 ttggggggat atctgtacga tgtgcgtaaa aatggcacta actgggagct ttacgcttcc
    38641 gggacagttc cggaaccgac tcctaatcct gaacccacac cagctcccgc tcagcctccc
    38701 atagtcaacc ccgatcctac gcctgaaccc gctcccacgc ctaaacccac cacgaccgca
    38761 gatgctggcg gcaattatct caatgtcggt tacttattga actatgttga aaaccgtacg
    38821 ctgatgcaac ggatgggtga cttgcgaaat cagagtaaag acggtaatat ctggttgcgc
    38881 agttatgggg gaagcctgga ctcctttgcc agtggcaaac tgagcggctt tgacatgggt
    38941 tacagcggta tccagtttgg tggggataaa cgtctctctg atgtaatgcc gttgtatgtc
    39001 ggtctgtata ttgactcaac acatgcatcg ccggactata gcggaggcga cggtaccgca
    39061 cgttcagact acatgggaat gtacgccagt tacatggcac aaaacggttt ttacagcgat
    39121 ctcgttataa aagcatcgcg ccagaaaaat agtttccacg tactggacag tcagaacaac
    39181 ggcgttaacg ccaacggcac tgcgaatgga atgagcatct ccctggaagc cgggcagagg
    39241 ttcaacctgt cccctactgg ttatgggttc tatatagagc cgcaaaccca gcttacatac
    39301 agccaccaga atgagatggc tatgaaggcg agtaatggcc tcaatataca tctgaatcac
    39361 tacgaatcgc tgctggggcg tgccagcatg atactggggt atgacatcac cgcaggcaac
    39421 agccagctga atgtctatgt gaagactggc gctatccgcg agttttcagg ggataccgaa
    39481 tatctgttga acgactcccg ggagaagtac agtttcaaag gtaatggctg gaataacggc
    39541 gtgggagtca gtgcacagta taacaaacag cacacattct atctcgaagc ggattacacg
    39601 cagggtaacc tctttgatca gaagcaagtc aacggaggat atcgcttcag cttttaagtg
    39661 cgggatagac aagcccttaa acgatgctga aaattgttag cgtgcgccgg aatgaaatac
    39721 agatcactct gatgtatcag aaatcgtttc tgtctttcat acccggcgct taaactgccc
    39781 tgcatcaatg ccatgttgct tcattttccg ccacagcgta gtgcggccga tcccaagtaa
    39841 agccgacatt tcctgaatgc gaccgcctgt gacctgggct gcgttaataa ttgcctcttt
    39901 ttcaacttcc gcaaatgaca gactggtgga aaggcgggtg gcgctgacat catctgtcgc
    39961 ctgctcggta aacagatgtt caggtaaatc actgacgcga atgcgcccgt tatcactact
    40021 cagagcaaga ttctcgatga cgctgtaaag ttcaaaatcg ttgcctggcc atgcacaaga
    40081 aactaggcga gcgagggcat cgtcatcaat tttcagccgc gtagagaagc gtttttcaag
    40141 actgcgtaat ttgttattca ccagcgccgg aatgctgcca cgccgcatac gcagaggcgg
    40201 gatggtaatt tcaaatgcat gcagcgcgta atacagctgg cgactaaaac gattttgttc
    40261 caccagcatt gcgaggtccg cggtcgttgt tgcaatcact ttgacatcaa ttggtattaa
    40321 acgccgcgca tccagtcgcg tgataacccc ctgcttgata acctgcagca aagcagactg
    40381 taactccacc gccagatatt caatcttttc aagaaacagc gtgccgccgt gtgccagttc
    40441 cagccgactc agacggccat tttcattgtc cgtgcgatcg ccaccaataa attcttccgc
    40501 cagcgcagca tcaccatata actcacaatt gacggcgata taaggacctg cagcacgctc
    40561 gctttcatta tgaattgcct gacttagcag tgccttgccc acgccctctt ctccacaaag
    40621 caggacagga aagctactgc gcgccgcctg gcgaccaaaa tgaatcaagc ggcgggtttg
    40681 cggatcgtcc tgtggcatat gagcgaaggt atggctgact tttcctaatt gactggtcat
    40741 taactgccgc atctgttcca caggatggag caacaaaata aagctggttc cctgcgtttc
    40801 tattatcggt ttaagggtta tcaccgcatc aataaactgg tgctggcttt caaaggttgc
    40861 ttctacgtgt ttgagcggat gtgccagttt tattgcttgt tgcaatacgg cgggtaacgt
    40921 taagagttca gtgattgccc gtccctgact tgccgtcgcg tcaaggcgca agacccgcgc
    40981 cgcctgggca ttaataaatt gcaaattacc ctgctcgtcc cagctaatca cgccatcatc
    41041 catactttct aacagggcat taagttgatt taaatgacgg ttagtttcag cgagcaaact
    41101 gtccgtcagc agtaaattac cgacctcgcg ggcgattgcc aacgtcaacg gcaaatcagc
    41161 tgcggtagtt tgctcaaccg gacacgccag cgctattgtt cccgtcaatc ggcccttgct
    41221 gtcaaacaac ggcgttgcac aaaaggccca gttccagagt gcctgtttga aatgttgatc
    41281 ggccatcgtt ttcacggcct gaccagagat agccgctaac gatagcgcac aagtaccaat
    41341 aattccctcg gcgcaatacg tgccgtcatt gaatcccagt gcacttagct gctgcaaggt
    41401 ttgcggatcg ccattacggc tgagaatgca ggcggtttca tcgaggataa acagcgcaca
    41461 ctctcgcggt gccatatatt cccaggcatc ttccagcgca gcctgcccga gcgtcagcat
    41521 cgctttctta cgccgataaa tagaagcaaa tgtcacgccc tgggcctgat gtggtacgtt
    41581 ccatgtctcc cgtttcatca gcttattgca tcgctcccag gaggttgcaa ttaagggaga
    41641 tatgccccga ccatcgttgt taaaagcgcc actcatatcc cgcatccctt atgacaaaag
    41701 gtgttccggc tcgcaatttt aagtattaat acacgtaaaa gtttcaatat ggaacattca
    41761 ttaaagcata tgtttcacta tgaaacataa tttgggctaa ttttcttttc ccttgcctga
    41821 tgcacaatgg attcatcggc agtcagtggt cgccgtgtcg ttgaacatca tccatgccct
    41881 accgtaattg ctggagcaaa ataatgaaaa aattgatcaa tgatgtgcaa gacgtactgg
    41941 acgaacaact ggcaggactg gcgaaagcgc atccatcgct gacactgcat caggatccgg
    42001 tgtatgtcac ccgagctgat gcccctgttg caggaaaagt cgccctgctg tcgggtggcg
    42061 gcagcggaca cgagccgatg cactgtggtt atatcggtca ggggatgctt tcgggggcct
    42121 gtccgggcga aattttcacc tcaccgacgc ccgataaaat ctttgaatgc tccatgcaaa
    42181 ttgatggcgg cgaaggtgta ctgttgatta tcaaaaatta caccggcgat attcttaact
    42241 ttgaaacagc gaccgagtta ctgcacgata gcggcgtaaa agtgaccact gtggtcattg
    42301 atgacgacgt tgcggtaaaa gacagtcttt ataccgccgg gcgacgcggc gttgccaaca
    42361 ccgtattaat tgaaaaactc gtaggcgcag cggcggagcg tggcgactca ctggacgcct
    42421 gtgcggaact ggggcgtaag ttgaataatc aaggccactc aataggtatc gctctcggtg
    42481 cctgtaccgt tcctgccgcg ggcaaacctt cttttaccct tgcggataat gagatggagt
    42541 ttggcgtagg cattcatggt gagccaggta ttgaccgccg ccctttctct tcccttgatc
    42601 aaaccgtcga tgcaatgttc gacaccctgc tggaacatgg ctcataccat cgcactttgc
    42661 gtttctggga ttatcaacaa ggcagctggc aggaagaacc acaaaccaaa caaccgctcc
    42721 agtctggcga tcgggtgatt gcactggtta acaatcttgg cgcaactccg ctttctgagc
    42781 tgtacggcgt ctataaccgc ctgaccacac gttgccagca agcgggattg actatcgaac
    42841 gtaatttaat tggcgcgtac tgcacctcac tggatatgac cggtttctca atcaccttac
    42901 tgaaagttga tgacgaaacg ctggctctct gggacgcccc ggtccacacc ccggccctta
    42961 actggggtaa ataaggagaa agcaatgtca ctgagcagaa ctcaaattgt taactggctc
    43021 actcgttgtg gcgatatttt caacaccgag agcgagtatc ttaccgggct ggatcgcgag
    43081 attggcgacg ctgaccacgg gctaaatatg aaccgaggct ttagcaaagt ggtggaaaaa
    43141 ctccctgcta tcgcagataa agatatcggt ttcattctca aaaataccgg tatgacgctg
    43201 ctttccagcg tcggtggtgc cagtggtccg ctgttcggta ccttctttat ccgcgccgca
    43261 caggcgaccc aggcacggca aagcctgaca ctggaagagc tttatcagat gttccgcgat
    43321 ggcgcggacg gcgtaatcag tcgcgggaaa gccgaacctg gcgataaaac catgtgtgat
    43381 gtgtgggtgc cggtggtgga atcgttacgt cagtccagcg agcaaaatct ctctgttccg
    43441 gtggcgctcg aagctgccag tagcatcgcc gaatccgctg cacaaagtac gattacgatg
    43501 caagcccgca aaggccgcgc cagttatctc ggtgaacgca gtattggtca ccaggatccc
    43561 ggcgcgacct cggtgatgtt tatgatgcaa atgttggcgt tagccgcaaa agagtaagga
    43621 attggtgatg gtaaacctgg tcatagtttc acatagcagc cgactgggag aaggtgtcgg
    43681 tgaattagcc cgtcagatgt taatgagtga tagttgtaaa atcgccattg ccgcgggaat
    43741 tgacgatcca caaaatccca ttggtaccga tgccgtcaaa gtgatggagg ccatcgaatc
    43801 tgttgctgat gccgaccatg tgctggtcat gatggatatg ggtagcgcat tattgagtgc
    43861 tgaaactgcg ctggaattgc tggctcccga gatcgccgca aaagtacgtt tgtgtgctgc
    43921 gccgttggtc gaaggtacac tggcagcaac ggtcagcgcg gcctcggggg cggatatcga
    43981 caaagttatc tttgacgcta tgcatgcgct ggaagccaaa cgtgaacaac tgggtttacc
    44041 gtcctccgac actgaaatct ctgacacatg tcctccgtac gatgaagaag cccgttctct
    44101 ggcggtggtc ataaaaaacc gtaacggcct gcatgtacgt ccggcctccc ggctggttta
    44161 taccttatcg acatttaatg ccgatatgtt gctggaaaaa aacggcaaat gcgtcacacc
    44221 agagagtatt aaccagattg cgttactaca agttcgctat aacgatacgc tgcgcctgat
    44281 tgcgaaaggg ccagaagctg aagaggcact gatcgctttc cgtcacctgg ctgaagataa
    44341 ctttggtgaa acggaggaag tcgctccacc tactctgcgt cccgttctgc ctgtttcggg
    44401 taaagccttt tattatcaac cagttttatg tacggtacag gcaaaatcaa ccctgaccgc
    44461 ggaagaagaa caagatcgat tacgccaggc tattgacttc acgttattag atctgatgac
    44521 gttaacagcg aaagcagaag ccagcgggct tgacgatatt gccgcaatct tttctggtca
    44581 ccatacactg ttaggtgatc cggaactgct ggcggcggca agcgaactcc ttcagcatga
    44641 acattgcacg gcagaatatg cctggcagca agttcttaaa gaacttagcc agcaatacca
    44701 gcaactggat gatgaatatc tacaagctcg ctatattgat gtggacgatc ttctgcatcg
    44761 caccctggtc cacctgaccc aaacgaaaga agaactcccg cagtttaact cgccaactat
    44821 tctactggcg gagaacattt atccttccac agtactgcaa ctggatccgg cggttgtaaa
    44881 aggtatctgc cttagcgccg gaagtccggt atcccacagc gccctaatcg cccgtgaact
    44941 ggggattggc tggatttgcc agcagggtga gaaactgtat gcgatacaac cagaagaaac
    45001 gctaacgctg gacgttaaaa cgcaacgttt caaccgtcag ggttaagcca ccgttcggat
    45061 ggcatcgttc tgatgtcatc cggcaattta cccagcactg atgtttttta actacctgtt
    45121 atatttttta ccaatttatg atgatttttc ttcgctaatc gcgaaccaaa tgtcgctggc
    45181 tggctaataa acagacgtta tttgatcact ctatttcgcc gttatttatc cctttaattc
    45241 ccttttctaa aatgcctgac cgttcgcaga atgagatttc gatcatgcag ctagtgcgat
    45301 cctgaactaa ggttttctga tacttgaata ccgttttatt cggtttcgcc aaaggagaat
    45361 gattgatgaa atcccccgca ccttctcgcc cgcaaaaaat ggcgttaatt ccagcctgta
    45421 tctttttgtg tttcgctgcg ctatcggtgc aggcagaaga aacaccggta acaccacagc
    45481 cgcctgatat tttattaggg ccgctgttta atgatgtgca aaacgtcaaa ctttttccgg
    45541 accaaaaaac ctttgccgat gccgtgccta acagcgatcc gctgatgatc cttgctgatt
    45601 atcggatgca gcaaaaccag agcggatttg atctgcgcca tttcgttaac gtcaatttca
    45661 ccctgccgaa agaaggcgag aaatatgttc cgccagaggg gcagtcactg cgcgaacata
    45721 ttgacggact ttggccggta ttaacgcgtt ctaccgaaaa caccgaaaaa tgggattctc
    45781 tgttaccgct gccggaacct tatgtcgtgc cgggcggacg ttttcgcgag gtatattact
    45841 gggacaatta cttcaccatg ttaggacttg ccgaaagtgg tcactgggat aaaatcgcgg
    45901 atatggtggc caattttgct catgaaataa acacttacgg tcatattccc aacggcaacc
    45961 gcagttacta tttaagccgc tcgcaaccgc ccttctttgc cctgatggta gagttactgg
    46021 cgcagcatga aggcgatgcc gcgttgaagc aatacctgcc gcaaatgcaa aaagaatatg
    46081 cttactggat ggacggtgtt gaaaacctgc aagccggaca acaggaaaaa cgcgttgtaa
    46141 aacttcagga tagtaccctt ctcaaccgct actgggacga tcgcgatacg ccacgaccag
    46201 agtcatgggt ggaagatatt gccaccgcca aaagcaatcc gaatcgacct gccactgaaa
    46261 tttaccgcga cctgcgctct gccgctgcgt ctggctggga tttcagctcg cgctggatgg
    46321 acaacccgca gcagttaaat accttacgca ccaccagcat cgtaccggtc gatctgaaca
    46381 gcctgatgtt taaaatggaa aaaatcctcg cccgcgccag caaagctgca ggagataacg
    46441 cgatggcaaa ccagtacgaa acgctggcga atgcccgtca aaaagggatc gaaaaatacc
    46501 tgtggaacga tcaacaaggc tggtatgccg attacgacct gaaaagtcat aaagtgcgca
    46561 atcagttaac cgcggccgcc ctgttcccgc tgtacgtcaa tgcggcagcg aaagatcgcg
    46621 ccaacaaaat ggcgacggcg acgaaaacac atctgctgca acccggcggc ctgaacacca
    46681 cgtcggtgaa aagtgggcaa caatgggatg cgccaaatgg ctgggcaccg ttacagtggg
    46741 tcgcgacaga aggattacaa aactacgggc aaaaagaggt ggcgatggac attagctggc
    46801 acttcctgac caatgttcag cacacctatg accgggagaa aaagctggtg gaaaaatatg
    46861 atgtcagcac caccggaacg gggggcggcg gtggcgaata tccattacag gatggctttg
    46921 gctggaccaa tggcgtgacg ctgaaaatgc tggatttgat ctgcccgaaa gagcaaccgt
    46981 gtgacaatgt tccggcgacg catccgaccg ttaagtcagc aacgacgcaa ccctcaacca
    47041 aagaggcaca acccacacct taaccagcgc ttactccgac aacgtcattc ggcttctccg
    47101 gatggcgttg tcggcagata acaccatatc tatggttttt tctacacaga cggtttttca
    47161 agtcctggcg ggaagtattg cgcaaatgtt tgccatacgt ggttgccgaa gatagcgtca
    47221 tttctacggg taagtgtttc actttcatca cccggcgatt tacgccgacg ttggctatat
    47281 aaagcatcaa ttgctaccga atattgttac ggtgttcact catcaacgtt tttttgcttt
    47341 ctggagttaa attaataatc acataatcca gacactccag agaatttagc aaccaaaaaa
    47401 agaattcaga ccattcctct tcgcgtaact gaagcattgc cgaacgggta ttttggctta
    47461 atctgccaga aggattgatc aattctaaat gttcaataag cgcagccaaa accgcctcac
    47521 tatccgcccc atttttaacg cctgactggc tactgtctaa aatatttatt ttctgaatca
    47581 caccaaaaac aacgaaacaa taatta
//
LOCUS       DAJTQQ010000024        47501 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000024 DAJTQQ010000000
VERSION     DAJTQQ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.024"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            172..654
                     /gene="sseA"
                     /locus_tag="OO309_001464"
                     /pseudo
     CDS             172..654
                     /gene="sseA"
                     /locus_tag="OO309_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
     gene            complement(1473..2249)
                     /gene="sseB"
                     /locus_tag="OO309_001465"
     CDS             complement(1473..2249)
                     /gene="sseB"
                     /locus_tag="OO309_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR5846650.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2391..3674)
                     /gene="pepB"
                     /locus_tag="OO309_001466"
     CDS             complement(2391..3674)
                     /gene="pepB"
                     /locus_tag="OO309_001466"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR5846651.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3733..3933)
                     /gene="iscX"
                     /locus_tag="OO309_001467"
     CDS             complement(3733..3933)
                     /gene="iscX"
                     /locus_tag="OO309_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR5846652.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3945..4280)
                     /gene="fdx"
                     /locus_tag="OO309_001468"
     CDS             complement(3945..4280)
                     /gene="fdx"
                     /locus_tag="OO309_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR5846653.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4282..6132)
                     /gene="hscA"
                     /locus_tag="OO309_001469"
     CDS             complement(4282..6132)
                     /gene="hscA"
                     /locus_tag="OO309_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR5846654.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(6149..6664)
                     /gene="hscB"
                     /locus_tag="OO309_001470"
     CDS             complement(6149..6664)
                     /gene="hscB"
                     /locus_tag="OO309_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR5846655.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6760..7083)
                     /gene="iscA"
                     /locus_tag="OO309_001471"
     CDS             complement(6760..7083)
                     /gene="iscA"
                     /locus_tag="OO309_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR5846656.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(7100..7486)
                     /gene="iscU"
                     /locus_tag="OO309_001472"
     CDS             complement(7100..7486)
                     /gene="iscU"
                     /locus_tag="OO309_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR5846657.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7514..8728)
                     /gene="iscS"
                     /locus_tag="OO309_001473"
     CDS             complement(7514..8728)
                     /gene="iscS"
                     /locus_tag="OO309_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR5846658.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8840..9328)
                     /gene="iscR"
                     /locus_tag="OO309_001474"
     CDS             complement(8840..9328)
                     /gene="iscR"
                     /locus_tag="OO309_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR5846659.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9598..10338)
                     /gene="trmJ"
                     /locus_tag="OO309_001475"
     CDS             complement(9598..10338)
                     /gene="trmJ"
                     /locus_tag="OO309_001475"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR5846660.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10457..11260
                     /gene="suhB"
                     /locus_tag="OO309_001476"
     CDS             10457..11260
                     /gene="suhB"
                     /locus_tag="OO309_001476"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR5846661.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11405..12259
                     /locus_tag="OO309_001477"
     CDS             11405..12259
                     /locus_tag="OO309_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5846662.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12450..13730
                     /gene="csiE"
                     /locus_tag="OO309_001478"
     CDS             12450..13730
                     /gene="csiE"
                     /locus_tag="OO309_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR5846663.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13722..14861)
                     /locus_tag="OO309_001479"
     CDS             complement(13722..14861)
                     /locus_tag="OO309_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR5846664.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(15021..15911)
                     /gene="hcaR"
                     /locus_tag="OO309_001480"
     CDS             complement(15021..15911)
                     /gene="hcaR"
                     /locus_tag="OO309_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR5846665.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            16047..17408
                     /locus_tag="OO309_001481"
     CDS             16047..17408
                     /locus_tag="OO309_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR5846666.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17405..17923
                     /locus_tag="OO309_001482"
     CDS             17405..17923
                     /locus_tag="OO309_001482"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR5846667.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17923..18243
                     /locus_tag="OO309_001483"
     CDS             17923..18243
                     /locus_tag="OO309_001483"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR5846668.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18240..19052
                     /gene="hcaB"
                     /locus_tag="OO309_001484"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18240..19052
                     /gene="hcaB"
                     /locus_tag="OO309_001484"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR5846669.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            19062..20284
                     /locus_tag="OO309_001485"
                     /pseudo
     CDS             19062..20284
                     /locus_tag="OO309_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            20381..20803
                     /locus_tag="OO309_001486"
     CDS             20381..20803
                     /locus_tag="OO309_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5846670.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20851..21723)
                     /locus_tag="OO309_001487"
     CDS             complement(20851..21723)
                     /locus_tag="OO309_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR5846671.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21735..22829)
                     /locus_tag="OO309_001488"
     CDS             complement(21735..22829)
                     /locus_tag="OO309_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5846672.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22862..23860)
                     /locus_tag="OO309_001489"
     CDS             complement(22862..23860)
                     /locus_tag="OO309_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5846673.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23885..25396)
                     /locus_tag="OO309_001490"
     CDS             complement(23885..25396)
                     /locus_tag="OO309_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5846674.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25419..26401)
                     /locus_tag="OO309_001491"
                     /pseudo
     CDS             complement(25419..26401)
                     /locus_tag="OO309_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(26498..29599)
                     /locus_tag="OO309_001492"
     CDS             complement(26498..29599)
                     /locus_tag="OO309_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR5846675.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWVNPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKKPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29606..29779)
                     /locus_tag="OO309_001493"
     CDS             complement(29606..29779)
                     /locus_tag="OO309_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846676.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29897..31090
                     /locus_tag="OO309_001494"
     CDS             29897..31090
                     /locus_tag="OO309_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR5846677.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31288..32541)
                     /gene="glyA"
                     /locus_tag="OO309_001495"
     CDS             complement(31288..32541)
                     /gene="glyA"
                     /locus_tag="OO309_001495"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR5846678.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32868..34058
                     /gene="hmpA"
                     /locus_tag="OO309_001496"
     CDS             32868..34058
                     /gene="hmpA"
                     /locus_tag="OO309_001496"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR5846679.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(34103..34441)
                     /gene="glnB"
                     /locus_tag="OO309_001497"
     CDS             complement(34103..34441)
                     /gene="glnB"
                     /locus_tag="OO309_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR5846680.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34502..35836)
                     /gene="glrR"
                     /locus_tag="OO309_001498"
     CDS             complement(34502..35836)
                     /gene="glrR"
                     /locus_tag="OO309_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR5846681.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35826..36539)
                     /gene="qseG"
                     /locus_tag="OO309_001499"
     CDS             complement(35826..36539)
                     /gene="qseG"
                     /locus_tag="OO309_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR5846682.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36704..38131)
                     /gene="qseE"
                     /locus_tag="OO309_001500"
     CDS             complement(36704..38131)
                     /gene="qseE"
                     /locus_tag="OO309_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR5846683.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38689..42576)
                     /gene="purL"
                     /locus_tag="OO309_001501"
                     /gene_synonym="purI"
     CDS             complement(38689..42576)
                     /gene="purL"
                     /locus_tag="OO309_001501"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR5846684.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42835..44391
                     /gene="mltF"
                     /locus_tag="OO309_001502"
     CDS             42835..44391
                     /gene="mltF"
                     /locus_tag="OO309_001502"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR5846685.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44388..44924)
                     /gene="tadA"
                     /locus_tag="OO309_001503"
     CDS             complement(44388..44924)
                     /gene="tadA"
                     /locus_tag="OO309_001503"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR5846686.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44949..45584)
                     /gene="yfhb"
                     /locus_tag="OO309_001504"
     CDS             complement(44949..45584)
                     /gene="yfhb"
                     /locus_tag="OO309_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR5846687.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45793..46641
                     /locus_tag="OO309_001505"
     CDS             45793..46641
                     /locus_tag="OO309_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR5846688.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46980..47501)
                     /locus_tag="OO309_001506"
                     /pseudo
     CDS             complement(46980..47501)
                     /locus_tag="OO309_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11324 a        13021 c        11953 g        11203 t
ORIGIN      
        1 acgttacaga cggtcccggc aaaagtgcct gacctgaccc ctattagcgg ttaccagcgc
       61 cccaccagac ccggtgacat ccccccccgg atcatggacg actgggggca gtaatcatgc
      121 cgggtctggc tggctaacac cccattataa ggggtacgaa taaagtaacg gggacttgca
      181 ggctggcagc gcgatgacct gctattagaa gaaggtgcag tagagctgcc ggaaggagag
      241 tttaacgccg cgtttaatcc tgaagcagtg gtgaaagtaa ccgatgtgtt attggcaagc
      301 catgaaaata cggcgcaaat tattgatgcc cgcccggctg cacgttttaa cgcagaagtt
      361 gatgaacctc gcccaggttt acgtcgcgga catattcccg gtgcattgaa cgttccgtgg
      421 acggaactgg tgcgcgaagg cgaactaaaa acgaccgatg aactggatgc gatatttttt
      481 ggtcgcggcg tcagctatga caaaccgatt atcgtcagct gcggctctgg tgtaacggca
      541 gccgtggttt tgttagcact cgcgacgctg gatgtgccaa acgtgaaact gtacgacggc
      601 gcatggagtg aatggggcgc gcgggcagat ttaccggttg agccagtgaa ataagtatat
      661 tacaggcaat aaaaaaccgc cgaatttggc gtttttttat tgctggtctg gttcgcggcc
      721 tttccagcag gttgacttgt gttacatgag caacgcaggt gcttcacagc aaaacaatac
      781 tcaccagtaa ctctcttttt gtcaagcaaa agagagtaat tattgtttat ttagcgtatt
      841 atcgacaccg gccctttccg ccgtgttcgg taataaaata acctggctta ttagtccgaa
      901 ttcagacaaa tataaacaaa tcctgctcaa aattaaaaat tctaaccggt aaaagatatt
      961 acttaaacat gtaaatttac tttcctttaa aaaacaaaaa accgccaaaa tcaggcggtt
     1021 atttgttgct ggtccggttc gcggcctttc cagcaggttg tattaccgta gtaatgcaag
     1081 cgcgtctcag cggagacaat actcgccagt aactctcttt ttgtcaagca aaagagagtt
     1141 attattgttc tgttagtgta ttatccactg cggccctttc cgccgtctcg caagcgggcg
     1201 ctggcttaag gaaaggatgt tccgcgaccg ttagagcaag cgcgtctcag cgcttgctat
     1261 cgcggcaata tcgccagtgg tgctgtcgtg atgcggtctt cgcatggacc gcacaatgaa
     1321 gatacggtgc ttctgtatcg tacttattgt ttctggtgcg ctgttttccc aagtaaataa
     1381 taaccggagt ctctccggcg acaatttact ggtggttaac aaccttcaga gcagcaagta
     1441 agcccgaatg ccgcccttta gggcggcata ttttagatta tccggttttg tttaaagtca
     1501 cgcaaaaaac caccccagcg acgttcatag aatggcgcaa tatgttcggt aataaagtgg
     1561 ctaattcctt tttccccttt tttcacctga caaatatcga ttggttcatc gccaggtaat
     1621 gtatcggtcg ctacacttcc cgtcgcctga ataatttctt cgatatcacc atcggcttca
     1681 atgccaataa gtaaattagg ctgtgcctct tcgttctctt taattgaaca aataaaagca
     1741 cgcttcaccg gcttaatggt tttaaataag gtggtgagtg aatcaatcat ttgtgctggc
     1801 ggctctgcga cttccgataa tatcagcgat tcaccgcctt ccaggatttc ctggctgctc
     1861 agcggatttc cctcttcacc aatcaacaaa ctgatttcac gcggcataaa ttctttaccg
     1921 gtcggcagtt tggcattaag gaagagcgtt tcgccaaggg tcatctcaaa caatgtgcga
     1981 acgggcatta cgacaaatgc ctgttcgtct tcaaccgcct gttgaagcgc ttctaacgag
     2041 gtgaaaaaag gaatgacgct ggtgccgtct tctttttccc agtgctgtaa atcaagcgca
     2101 ctatcttcaa ccacagcctc gccctgcgcc gccgtaccag gcacccagac ggtggattcc
     2161 agtagagtac ggaaaaaggc cgggcggtgc gccggttcgg ttgctgcttt ttccagcagg
     2221 tcttcaagtt cgttttttgt ttcggacata agaaccacaa ttcattcaac gttcggggcg
     2281 caaatgcctg atgcgctacg cttatcaggc ctacaaggga ttcgcaattt gttgaatttg
     2341 cagaatttgt aggccggata aggcgtttac gccgcatccg gcatattagt ttacgccgtt
     2401 aacagattag ctatcgtgcg cacaccaagt cccgtagcgc ccgcagacca ctgttcaacc
     2461 ggcgctttac ggtaagtcgc cgagcagtcg atatgcagcc agccttgctg atagttctca
     2521 acaaagtgcg acaggaagcc cgccgccgtg ctcgcgcctg ccggatacgc cgcgcttccg
     2581 gtattgttca gttcggcaaa gttagacggc agctggctgc ggtggaattc cgccagcggc
     2641 agacgccaga atggttcgtt ctcttgtgag gcactcgcca gtaaacgacc ggcaagcgca
     2701 tcgtcaaaac tgaacagcgc gtgataatca ttacccagcg cagttttcgc cgccccggtg
     2761 agggtcgccg catcaatgat catttccggt ttctgcgcac tggcatcaat cagaccatcg
     2821 gcaagcacca gacgcccttc ggcatcagtg ttcatcactt caactttttt accgttgcga
     2881 taggtgatga tatcgcccag cttgaacgca ttgccgctaa tcaggttatc cgcacagcag
     2941 aggaacagct tcacgcgctt gttcagtccg cgcataatgg caaatgccag cgccccggta
     3001 accgttgccg cgccgcccat gtccgacttc atcgagtcca taaacgcagt ctgtttgatg
     3061 ctgtagccgc cggagtcaaa agtgatacct ttacctacca ggcacgcgta caccggtgct
     3121 tctttatcgc cagttgggtt gtaatccagc gccagcaata ccggagaacg ttctgaaccg
     3181 cgcccgacag tgtgcagccc catataacct tgctcacgca gatcttcgcc tttggtgatc
     3241 cgataagtca cacgatcgcc cgcgacgttg ctgatcagat caacagcacg ctgtgccagt
     3301 tgcgatggtc ccaattcttc tgccggtgcg ttgatggtgt cacgcaccca gtcgatgatc
     3361 atcaggcggt tatccagttc ctggcgctgg gcatcgtcca gatccggcca ctccacttta
     3421 cgcgtgcctt tcgggccttt ataaccttgc cagaatgccc agcagcgatc cgcatcccag
     3481 ccttcaccgc ttaactgaac atgcttgatg cccagaccgt caatcttgcg ggccgcacgc
     3541 tggatcagcc ccagatcgtc tgccccgttc aaatgcaggg taatgccgtc attattaatg
     3601 ctgtaagttg ctttttctcc ccagcgcgcg tcggcaggtt gggtagagag ggtaatcttc
     3661 atcgcttctg tcattttagt tatccttatt cgtttaacag caaaacgggc tacccccagg
     3721 cagcccgttc attcactcgg cttcgtccag ccagactaac aaaatcgctt cgaggatttt
     3781 ctcgttggat gcctgcgggt cgtcgtcgaa atcttccaga tcgcaaatcc actgatgcat
     3841 atcggtgaat cgaaccgttt tcggatcaag atcgggatac gcatcgtaca gtgcttcgcc
     3901 aatttcgcgg ctatcggtcc acttaagtcc catactaacc tctgttaatg ctcacgcgca
     3961 tggttgatag tgtaacgcgg gatttcgacc actaaatctt cgtcggtgac gcgcgcctgg
     4021 cagcttaaac ggctttccgg ctccagtccc caggctttgt ccagcatgtc gtcttcctgc
     4081 tctgagcttt ccggcagtga gtcaaaacct tcacgaacga tgcagtggca ggtggtgcaa
     4141 gcacaggatt tttcacaggc gtgttcaatc tcaataccgt tacgcagcgc tgcgtcgaga
     4201 atggtttcac cgctattagc ttccagaaca gcgccatcag ggcagaggtc ctgatgaggc
     4261 aaaataacaa tctttggcat attaaacctc gtccacggaa tggcctttca gcgcacgacg
     4321 aaccgactgg tccatgcggc gagcggcgaa atcctgggtt tgtttgtcta cgtttttaat
     4381 cgcttgttcg atggcgtcaa catcatcgcc ctgcgccact tcactcaggt gagcggcagc
     4441 attgtcaatg acctgacgct ctgcggcgct taacagcgcg gcatcagcag ccagcgcgcc
     4501 gtgcagactt tccagcacac gcgccgcttc tactttttgt tctgccagca tacgggcttt
     4561 tacgtcctgc tcggcatagc tcattgagtc tttgatcatc gaagcgattt cgctatcggt
     4621 cagaccgtaa gacggtttga cctgaataga cgcctcaacg ccggtggatt tctccatcgc
     4681 cgtcacgctc aaaagaccgt cggcatcgac ctggaacgtc acgcgaatat gcgcaccgcc
     4741 agccggtagc gccggaatac cacgcagcgc aaaacgcgcc agtgagcggc agtcctgcac
     4801 cagttcgcgc tcaccctgca ttacatggat agacatcgcc gtctgaccat ctttaaaggt
     4861 ggtgaaatcc tgagcgcggg ccaccggaat agtggtatta cgcggaatca ctttctccac
     4921 caggccgccc atcgtttcga ggcccagcga cagtgggatc acatcaagca gcagcatttc
     4981 gctgtctggc ttgttaccca ccagaatatc cgcctgaatc gccgcgccaa tagcgacgac
     5041 tttatccggg tcgatggaag tcagcggtgg acgaccgaaa aattcgccta cccgttcacg
     5101 caccagcggc acgcgagtag aaccgcccac catcaccact tccagcactt cgtcagcttc
     5161 tacacccgcg tctttcagcg cgcgacgaca agccagtaag gttcgtttta ccagtggcgc
     5221 gatcagttca ttgaattgtt cacggctgat ttcgccctgc cagcccgcaa cgttaacggt
     5281 cacggagtcc gcatcgctca gcgcgatttt ggctgcaatg gtggcatcca gcagttcacg
     5341 ctgaacgcgg ttatcgctac gatcaggaat gtccgcctgc tcgcgaatgt aatccgccag
     5401 cagatggtcg aaatcatcgc cgccgagcgc ggaatcaccg ccggttgcca gcacttcaaa
     5461 cacgccgcga cttaagcgca gaatggaaat atcaaacgtc ccgccaccga ggtcataaac
     5521 agcgatcacg ccttcctgac cggaatccag cccgtaggcg atagccgcag cagtcggttc
     5581 gttaagtaag cgcagaacat gcaaccccgc cagacgcgcc gcgtctttgg tgccctgacg
     5641 ctgggcatcg tcaaagtacg ccggaacggt gataactaca ccatccagct cgcctgccag
     5701 ggcttcagtt gcccgcgccg ccagtgcttt gaggatgtcc gcagaaacgc gcaccgggtt
     5761 cagtagcccc gccgccgttt caatcatcgg caggccgttt tcgctggcct ggaattgata
     5821 aggcagatgc ggatagcgtt gctggatatc agccagcgag cgccccatca ggcgtttaac
     5881 agaactaatg gtgttggcgg tgtcaagcgc tgcgttagta cgcgcgtcat aacccaccga
     5941 atgcccttgc tgttgatagt gaacaacaga tggcagcagg tgacggcctt catggtcggc
     6001 taacgtttcg gcctgaccgc tgcgcactgt cgccaccagc gagttggttg tgcccaggtc
     6061 aataccagcc gccagacgac gctgatgcgg cgcagcactc aaaccaggtt cactaatttg
     6121 taataaggcc atgtttagct tccagaaatt aaaaatcgag cagtttttct tcgagttgtt
     6181 cggcactgct ccgcagttta tcgagaaaac gcagcttacg caccgtatcc gccgccgcgt
     6241 cccacgcctc gttgtctaac tgttcaacca tcaactgatg gcgggtatca aacatctttt
     6301 tcacacgttt gataaagctt tccagccgcg cttcatcttt cgcctgttcg atctcgtcca
     6361 gctcttcgcg cagctccaac tgttccatca ggaacgcggt gtcgcgcaca gtatgctgct
     6421 cgctggcgag atcaaaaccg tgtaaagaaa gcaaatattc cgcgcgcatt aacggatgac
     6481 gcagcgtttg ccaggcctgg ttaatggttg cagattgctg tacggcggcg agttgttccg
     6541 ccaggcttcc gctggcgaat ttatcaggat gatactgacg ttgtagatcc tgaaaacgca
     6601 ggctcagtgc ctgggtatcg agttgataac gggcaggcaa gccaaagagg gtgaagtaat
     6661 ccataacatt ctcagggctg cgatccgccg cgataacggc gaatcaggta aaacaaaccc
     6721 cacgcgcagg cgaccacggt ggggttatcg gtatgcgcat caaacgtgga agctttcgcc
     6781 gcaaccacac tcatctttaa cgttcgggtt ggtgaattta aacccttcat tcaggccttc
     6841 ttttacgaag tccagctgcg taccgtccag aaattgcagg cttttgccat cgaccacgac
     6901 tttcacgcct ttgtcttcaa acacgatgtc ttccggcgtc ggttcgtcaa caaattccag
     6961 tacataagcc atacctgaac atccggaggt tctcacgccc agacgcaggc caaacccttt
     7021 gccgcggtta gccagaaagg tatttactcg cgctgctgca ctgtcgctca gtgtaatcga
     7081 cataaccaaa cctcaactct tattttgctt cacgtttgct tttatagtcc gcaatggcgg
     7141 ctttgatcgc gtcttctgcc agaatagaac agtgaatttt caccggcggc agttcaagtt
     7201 cttcagcaat atcggtgttt ttgatcgcct gcgcttcgtc gagagacttc cctttcaccc
     7261 attcggtgac cagggagctg gaagcgatag cggaaccgca gccgtaagtt ttaaaacgcg
     7321 cgtcttcaat gataccttca tcgttgactt taatctgcaa cttcattacg tcgccacagg
     7381 ccggtgcacc caccatgccg ctgccgacgt tctcgtcgtt gttatcaaag gaacccacgt
     7441 tacgcggatt ctcgtaatgg tcgataactt tttcgctgta agccattata aattctcctg
     7501 attccgatac cgattaatga tgagcccatt cgatgctgtt cagatccacg ccctgcttgt
     7561 acatttccca cagcggagaa aggtcacgca gacgaccgat ggatttacga actaactcga
     7621 tggtgtagtc gatctcttct tcagtagtaa aacgacctaa agagaaacgg atagagctat
     7681 gtgccagctc gtcgttcagc cccagcgcgc gcagcacgta ggacggttcg aggcttgctg
     7741 acgtacaggc ggaacctgaa gaaactgcga ggtctttcag cgccataatc agcgactcac
     7801 cttcaacgta gttgaagctg acgttgagaa tgttcggcgc accgtgttcc aggtcaccgt
     7861 tcaggtaaac ttcttcgata tctttgatgc cgttccacag acggttacgc aggccgcgca
     7921 gacgttccat ctcggtcgcc atctcttctt ttgcgatgcg ataggcttcg cccattccga
     7981 cgatctggtg aacaggcaga gtgccggaac gcataccgcg ctcgtgaccg ccgccgtgca
     8041 tttgcgcttc gatgcgtaca cgcggtttac gacgcacata cagcgcaccg atacctttcg
     8101 ggccatagat tttgtgaccg gagaaagaca tcaggtcaac tttcaactgg ctcaggtcga
     8161 ttggcagttt gcccacgctc tgggttgcat caacgtgata gataatgcca cgagcacggc
     8221 acatttcgcc gatagccgcg atatcctgca ccacgccgat ttcgttattt acgtgcatga
     8281 tggaaacaag gatggtgtcg tcacgcatcg ccgcttcgag ttctttcagg tcgataatac
     8341 cgttacgctg cggtgccagg taggtgactt cgaaaccttc gcgctccagc tgacggcaag
     8401 tatccagtac cgctttgtgt tcggttttgc tggtgatgat gtgcttgcct tttttctgat
     8461 aaaagttggc tgcacctttg atcgccaggt tgtcagattc ggttgcacca gaggtaaaga
     8521 cgatttcacg cggatcagcg ccgaccagat cggcaatctg attacgggcg atatctaccg
     8581 cttcttcagc ctgccagccg aaacggtgag aacgagaggc cgggttacca aaggttccgt
     8641 ccatcgtcat gaactgcatc attttctcgg caacacgcgg gtccaccggc gtggttgcgg
     8701 agtagtcgag ataaatcggt aatttcattg ctctataaac tccgtacatc actcaatgca
     8761 aggaatcagg ctaccggctg gatgtacgac cgtgtttacg gagtatttag cactccggcc
     8821 tgattctgaa ttctttttat taagcgcgta acttaacgtc gatcgcgtct tgtgtgcggg
     8881 tgcgtggcgc gtcgtgagta tgctgacgac cagacacatc cagcacttcc tggttattaa
     8941 ccagttcgcc taaagtaatg ttgttgagaa aaccggtgag acggtcgctc aaatcacgcc
     9001 acagcgcgtg ggtcaggcat ttatcgccgc cctggcagcc gcctttaccc tgacaacggg
     9061 tggcatctac agattcgtca acggcgctaa tcacttcgcc aacggcgatg ctgctggcat
     9121 ctttgcctaa cagataacca ccgcctggtc cacgtacgct ggaaaccaga ccatttttac
     9181 gcagacggga aaacagttgt tccagataag aaagggaaat tccctgacgt tcggaaatat
     9241 cagccaacgg taccgggccc gcttcagagt tgagcgcaac gtcaagcatt gcggtcacgg
     9301 catagcgccc tttagatgtc agtctcatgt cttacttcac ctcaaactcg cccctgcccg
     9361 gggtttttta ttgtaaaagt gggggtattg catagcaggg tcaagtctga cattcccgag
     9421 taaattggtc aactatttac ttgactgatt tagtcgggta tttaaccttc agtgccattt
     9481 ttttatcgtg gcgtttgtac ctgttgtcgg atgcggtgtg aacaccttat ccgacctacg
     9541 attctgtaca ttgtaggcct gataagacgc gttaagcgtc gcatcaggca accactgtta
     9601 ttcggccttg ttacctttat tctgctgctc aatagaagcc agaatcccgc gcaggatatt
     9661 caattcctgg ctttccgggc gcgcacgggt aaacagacgg cgcaatttat tcatcacctg
     9721 ccccggatgg ttttcacgga taaaaccggt tgccagcagc gtttgttcca gatgaccata
     9781 aaaacgctcc agatcatcga ccagcggata cggcgtctct tcatgctcga cctgctcgcc
     9841 attttcctga gttgccagcc aggccatacg cacttcatag gcaataacct gaaccgccat
     9901 tgccaggttc agcgaactgt attccgggtt tgccgcaatc gcgacatgat aatggcattt
     9961 ctgcaactct tcattggtca agccgacgcg ctcacgacca aacaccagcg ccaccggggt
    10021 atttgccgct tcagcgacgc ttttcagacc gcattcgcgc gggtcgagca tcggccacgg
    10081 cagcgtgcgg gagcgtgcgc tggtgcccac caccaggcta caaccggcta acgcttcgtc
    10141 gagcgtatcg acgatgtgag cattaccaat cacatcgctg gcccctgcag ccagggcaat
    10201 cgcctgggag tcgggtttca ccagtggatt aaccagccac agattggtta atcccattgt
    10261 tttcatggca cgggcaacag aacccatatt gccggtgtgt gacgtctcca ccagcacaat
    10321 tcgaatattt tgcagcattg tctttcttca tctaaagatt attcacgcat cttatcataa
    10381 aacgaagaca gatgccgatc tcgctgctat actctgcgcc gttttcccgt tctttaacat
    10441 ccagtgagag agaccgatgc atccgatgct gaacatcgcc gtgcgcgcag cgcgcaaggc
    10501 gggtaattta attgccaaaa actatgaaac cccggacgct gtagaagcga gccagaaagg
    10561 cagtaacgat ttcgtgacca acgtagataa agctgccgaa gcggtgatta tcgacacgat
    10621 tcgtaaatct tacccacagc acaccatcat caccgaagaa agcggtgaac ttgaaggtac
    10681 tgatcaggat gttcaatggg ttatcgatcc actggatggt actaccaact ttatcaaacg
    10741 tctgccgcac ttcgcggtat ctatcgccgt tcgtatcaaa ggccgcaccg aagttgctgt
    10801 ggtatacgat cctatgcgta acgaactgtt caccgccact cgcggtcaag gcgcacagct
    10861 gaacggctac cgtctgcgcg gtagcaccgc tcgcgatctc gacggtacca ttctggcgac
    10921 cggcttcccg ttcaaagcaa aacagtacgc cactacctac atcaacatcg tcggcaagct
    10981 gttcaacgaa tgtgcagact tccgtcgtac cggttctgcg gcgctggatc tggcttacgt
    11041 cgctgcgggt cgtgttgacg gtttctttga aatcggtctg cgtccgtggg atttcgcggc
    11101 aggcgagctg ctggttcgtg aagcgggcgg catcgtcagc gacttcaccg gtggtcataa
    11161 ctatatgctg accggtaaca tcgttgctgg taacccgcgt gttgtgaaag ccatactggc
    11221 gaacatgcgt gacgagttaa gcgacgctct gaagcattaa tgactcaggc gggtgatatc
    11281 actcacccgc cctcgccttt caggcgctat tccgaaatac ttcctcaccg ctttactttc
    11341 tttcgtcact ctcccaccat ctttccccga ttaatggatg ataaagaagt cgcaaggaaa
    11401 ggttatggca ctgccagtga acaaacgcgt tcccaaaatt ctgtttattc tctttgttgt
    11461 tgccttctgc gtttatttag tgccgcgcgt tgccatcaac ttcttctatt atcctgacga
    11521 taaaatttac ggtcccgacc cctggtcggc ggaatccgtc gaatttacgg ctaaggacgg
    11581 tactcgtctg caaggctggt ttattccttc ttcgacgggc cctgctgaca acgccatcgc
    11641 aaccatcatt catgctcacg gcaatgccgg aaatatgtcc gcccactggc cgctggtcag
    11701 ttggttacct gagcgtaatt tcaacgtttt tatgtttgat tatcgcgggt ttggtaaatc
    11761 aaaaggcact ccgtcccagg ccggattgct ggacgatacg caaagcgcca tcaatgtggt
    11821 gcgccatcgc agtgatgtaa acccacaaca gctggtgctg ttcgggcaga gtattggcgg
    11881 ggcgaatatt cttgctgtta ttggtcaggg tgatcgtgaa ggcatacgtg cggtgatcct
    11941 cgactccaca tttgcctctt atgcaaccat cgccaaccag atgatccccg gcagtggcta
    12001 cttacttgat gagagttaca gcggcgaaaa ttacatcgcc agcgtcagcc cgatcccgct
    12061 tttactcatt cacggtaaag ccgatcacgt tatcccatgg cagcacagcg aaaagttgta
    12121 tagcctggca aaagagccaa aacggttgat cctaatcccg gatggcgaac acattgatgc
    12181 tttttccgat cgtcacggca atgtttatcg cgaacagatg gtggacttta tccttagcgc
    12241 gttgaatccg cagaactaac ccatgatcgc tagcacgata atcattcaca aaaccacctt
    12301 aagacatgct aatccactgg tcagaacagt ttaaaatgag aaaaattctg tgacgcttgc
    12361 caacatttct gatgattagc attcccttcg ccatttccct gagcaaactt tagctattct
    12421 tatcaattat gcttatggga gatctacaga tgatgcctac gcttgctcca ccatctgtcc
    12481 tttcggctcc ccagcgccgc tgccagatct tgctgacgct ctttcagccg gggttgaccg
    12541 ccaccatggc aaccttcagc gagcttaatg gtgtggatga tgatattgcc agtcttgata
    12601 tcagcgaaac aggacgggag atcctgcgct atcatcaact cacactgacg actggttatg
    12661 acggtagcta ccgggttgaa ggtacagtgc ttaaccaacg tttgtgttta tttcactggc
    12721 tacgacgtgg tttccgtctg tgtccgtcat ttattaccag ccaattcacc cccgccctga
    12781 agagtgaact gaagcggcgc ggaattgcgc gtaactttta cgacgatacc aatctacaag
    12841 cgttagtgaa tctctgctcc cgacggctgc aaaaacgctt tgaatcgcgc gatattcatt
    12901 tcctgtgtct gtatctgcaa tattgtttgc tgcagcacca cgctggaatt acgcctcagt
    12961 ttaatccgct ccaacgtcgc tgggcggaat cctgccttga atttcaggta gcgcaggaaa
    13021 ttggacgcca ctggcagcgt cgggcgctcc agcatgtacc gcctgatgag ccactgttta
    13081 tggcattact tttttccatg ttgcgggttc ccgatccact gcgggatgcg catcagcggg
    13141 acagacaatt gcgtcagtct atcaaacgtc tggtaaacca ttttcgtgag ctgggaaatg
    13201 ttcgttttta tgatgaacag gggttatgtg atcagcttta tatccacctc gcccaggcgt
    13261 taaatcgcag tttgtttgcc atcggtattg ataataccct gccggaagag ttcgccagac
    13321 tgtatccacg cctggtacgc accacccgcg cggcgctggc cggatttgaa agtgaatacg
    13381 gcgtccatct ttctgatgag gaaagtggtc tggtcgcggt gattttcggt gcctggctaa
    13441 tgcaggaaaa cgatctgcat gaaaaacaga ttattctact gaccgggaat gatagcgagc
    13501 gagaagcgca gattgagcag cagttacgcg aactaacgtt actgccgctc aacattaagc
    13561 atatgtcggt aaaggcattt ttgcagacag gcgctccgcg tggcgcggca ctgattattg
    13621 cgccttatac catgccgtta ccgctctttt caccaccgct gatctatacg gacctgacgc
    13681 tgacaacaca tcaacaggag cagatccgca aaatgctcga atcagcatga gggaacaact
    13741 ttcgggcgca aaaacattgc cggaagcgcc accagcgcca ttacccagaa cacgccgtgg
    13801 cccagatatt gatacaggaa accggcgaaa acggtcatga tagcgatact gccgcccatc
    13861 gcgacggcag agtaaaccgc ctgtaaacgg atgacttcgc taacctggcg agcagcaata
    13921 taacgcatgg cggccaggtg gcagaccgtg aaggtgccgc aatgcagaat ttgcaccact
    13981 atcaaccacg gcaacgccgt agttgctccc ataatgcccc agcgcactac gccgcaaatc
    14041 gccgagatca acagcatatc gcgagcacta caacggcgga aaagtttatt actcagcgca
    14101 aagataatga cttccgccac cacgcccagc gaccacaaat accccaccgc cgaggccgag
    14161 tagccagctg cctgccagta aatggcgcta aaaccgtaat aggccgcatg tgccccctgc
    14221 aataaacaaa cgcaggccag aaagcgccag ttctggcgaa ccagcgccaa ccacgcagac
    14281 caaccggtgc tctcctgctg gcggcttgcc ccttgtggct gaatcgtcgg acggatgaga
    14341 aagccgagca gcatggatgc cacgcccaac gtcaacagca cgaggatcac ccgataatca
    14401 aacatagtga ccagtttgcc cgtcagcgcc gagccaatga caaacgccac cgagccccac
    14461 agtcgcactt tgccgtaatc aagcgggaac tgcttttgcc acgtattcgc cagtgcatcg
    14521 gtcaacggta ccagcggtga gaaaaagagg ttaaagccaa tcatcaccag catcagccac
    14581 gctacgtgcg cccccgccca gaaggcgaca gcaaagagaa gtggcagcag tgccagcacg
    14641 cgcaaggcgg aaatcaggcg ggaaggatcg ctgacgcggg gcgcgatgag caaactcccg
    14701 agaaaacggg caaccagacc tgcccccaat aacaggccga tggtttctgg cgttaaacca
    14761 atccctttaa gccagacgct ccagaaaggt aaaaaaatgc cgtaactaaa aaagtatgtg
    14821 aaatagccga gcgccaacca gcgcgtggat tgcaaaacca tgagtccctc ccgtcaggag
    14881 gcgttagtct ggggttaatc gaatgttggt gcaagttgaa aaataagaat attcgagcct
    14941 gcttcccgat gtctgtaacg ccagttcaca agcactaaaa aactgcgatc cgcgacgcat
    15001 gttgcaaaaa taatgcgtaa ttatgccgtt acgcttgcca aacgttcctg cacaatggcg
    15061 atgaaatcgc gtaatgccag cttcatctcc cctttcttcc acgccatcag taaagcaatg
    15121 gaaggcacat ttccggcaat gggacgaaaa acaacctgcc cggtattaaa attattcata
    15181 taaccgggta tcaaagtgac gcccagcccc atgcccacca gattcatggt caccagaata
    15241 ttcgttgcca cctggacgat atttggctgg ctgttttctt gcgcaaacca cgctttaacg
    15301 atcggcgcaa gcgaaccgga atacgccgga tcggtactga cgaaattcac gccatccagt
    15361 tgcgcggcgg tgatctcttt ttcatgtgct aaagggtgat caaccggtaa caccaccact
    15421 aatggttcgt caaaaagctc cagataatca atctccgggc tataaacagg atggcgcatt
    15481 aagccgacgt cgagttcacc acggcgaatt ttttcctcct gttgcgtggt gattaaactc
    15541 accagctcaa tcaaggtgtc tggctgtctg agacgaaaca tcggtaatac ttttggcaat
    15601 aaattcactt ccgccgatgg cacaaagcca atggttaatt gtctgtcttc ctgaacaatt
    15661 ttccgcgccc gtaatttggc attttccgct tgttcgagga ttgccagcgc gtcctggaga
    15721 aaacattctc ccgccgccgt caacgcgact ttgcgcttat ccctcaccag taacggaaca
    15781 ccgacacagt tttcaagatc gcggatctgg ctgcttaacg aaggctgtga ggtatgcagt
    15841 ttttccgccg cacgggtaaa gttcagtgcc tgcgccactg cgacgaaata gcgtaaatgc
    15901 cgtagttcca tcaccttccc cttgttatcg aaaaaacgtc tcagctggta gaaattaact
    15961 atttcacata ttagcaacca accagcaaca tccttatggc acaaaaatag aaggtcaata
    16021 catcttatct ttcaggatta aaaaatatga ccacaccctc agatttgaac atttaccaac
    16081 tgattgacac ccaaaacggt cgggtcactc cgcgtattta taccgacccg gacatttacc
    16141 aactggagct tgagcgtatt ttcggtcgtt gctggctatt tctcgcccac gaaagccaga
    16201 tcccaaaacc cggtgatttc tttaacacct acatgggaga agatgcagtt gtcgtggtgc
    16261 gccagaaaga cggcagcatc aaggcgtttc tcaaccaatg ccgccaccgg gccatgcgtg
    16321 tgagttatgc agattgtggc aacagtcgcg cctttacctg cccgtatcac ggctggtctt
    16381 atggcattaa cggcgagttg atcgatgtac cgctggaacc tcgcgcctac ccacaagggt
    16441 tgtgtaaatc ccactggggg ctaaacgaag ttccttgtgt ggagagttat aaaggactga
    16501 tttttggcaa ctgggatacc agcgcaccgg gcctgcatga ttacctgggt gacattgcct
    16561 ggtatctgga tggcatgctg gatcgtcgcg aaggcggcac cgaaattgtc ggcggcgtac
    16621 aaaagtgggt gatcaactgt aactggaaat tcccggcaga gcagttcgcc agtgaccagt
    16681 atcatgctct gttcagccat gcttctgccg ttcaggtatt aggggcgaaa gatgatggca
    16741 gcgataagcg cctcggtgat ggacaaaccg cccgcccggt gtgggaaacc gccaaagatg
    16801 cgctgcaatt tggtcaggac ggtcacggta gcggtttctt ctttactgaa aaaccggatg
    16861 ctaatgtctg ggtcgatggc gcagtttcaa gctattaccg cgaaacctat gccgaagcag
    16921 aacaacgttt aggtgaagtt cgcgccctgc gcctggcggg tcataacaat atattcccca
    16981 cgctttcatg gctaaacggc actgccacac tccgcgtctg gcatccgcgc ggccctgatc
    17041 aagttgaagt gtgggcgttc tgtattactg acaaagccgc ctccgatgaa gttaaagccg
    17101 cttttgaaaa cagcgccact cgtgcttttg gtcctgctgg ttttctcgag caggatgact
    17161 cggagaactg gtgtgaaatc cagaaattgc ttaaaggcca ccgcgcccgc aacagcaaac
    17221 tgtgtctgga aatggggctt ggtcaggaaa agcgccgcga cgacggcatt cctggcatta
    17281 ctaactatat cttttcagaa actgccgctc gtggaatgta ccaacgctgg gccgatcttc
    17341 tgagtagcga aagctggcag gaagtgctcg ataaaaccgc cgcttaccag caggaggtga
    17401 tgaaatgagt gcgcaagttt cactggagtt acatcaccgc attagccagt ttctctttca
    17461 cgaagccagt ctgctggatg actggaaatt tcgtgactgg ttggcacagc tcgacgaaga
    17521 gattcgttac accatgcgca ccacggttaa cgcgcaaaca cgcgaccgtc gcaaaggcgt
    17581 ccagccgccg acgacctgga ttttcaatga caccaaagac cagctggagc ggcgaatcgc
    17641 ccgtctggaa acgggcatgg cctgggcaga agagccgccg tcacgcactc gtcacttaat
    17701 cagcaactgc caggtaagcg aaaccgacat cccaaacgta tttgccgtga gggtgaatta
    17761 tctactttat cgggcacaaa aagagcgcga tgaaacattc tatgttggaa cgcgtttcga
    17821 caaagttcgc cgtctggaag atgacaactg gcgcttgctg gaacgggata tcgtcctgga
    17881 tcaagcggtg atcacttccc ataacctgag tgtactgttc tgatgaatcg aatttatgcg
    17941 tgtcccgttg cggatgtgcc ggagggtgag gctctccgga tagatacctc gcccgtcatc
    18001 gccttgttca acgtgggcgg cgagttttat gccattaacg atcgttgcag ccatggtaat
    18061 gcgtcgatgt cagaagggta tctggaagat gacgccacgg tggagtgccc gctacacgcc
    18121 gccagttttt gcctgaaaac ggggaaagcg ttatgcctgc ccgccaccga tccgctcacc
    18181 acttatccag tacacgttga aggtggtgac attttcatcg acttaccgga ggcgcagcca
    18241 tgagcgatct gcataacgag tccattttta ttaccggcgg cggatcggga ttagggctgg
    18301 cgctggtcga gcgatttatc gaagaaggcg cgcaagttgc cacgctggaa ctgtcggcgg
    18361 caaaagtcgc cagtctgcgt cagcgatttg gcgaacatat tctggcggtg gaaggtaacg
    18421 tgacctgtta tgccgattat caacgcgcgg tcgatcagat cctgactcgt tccggcaagc
    18481 tggattgttt tatcggcaat gcaggcatct gggatcacaa tgcctcactg gttaatactc
    18541 ccgcagagac gctcgaaacc ggcttccacg agctgtttaa cgtcaacgta ctcggttacc
    18601 tgctgggcgc aaaagcctgc gctccggcgt taatcgccag tgaaggcagc atgattttca
    18661 cactgtcaaa tgccgcctgg tatcctggcg gcggtggccc gctgtacacc accagtaaac
    18721 atgccgcaac cggacttatt cgccaactgg cttatgaact ggcaccgaaa gtgcgggtga
    18781 atggcgtcgg cccgtgtggt atggccagcg acctgcgcgg cccacaggcg ctcgggcaaa
    18841 gtgaaacctc gataatgcag tctctgacgc cggagaaaat tgccgccatt ttaccgctgc
    18901 aatttttccc gcaaccggcg gattttacgg ggccgtatgt gatgttggca tcgcggcgca
    18961 ataatcgcgc attaagcggt gtgatgatca acgctgatgc gggtttagcg attcgcggca
    19021 ttcgccacgt agcggctggg ctggatcttt aaggaagcac gatgaaagaa aaaacgatca
    19081 ttattgtcgg tggcgggcaa gcggcggcaa tggctgcggc ctcgctacgc cagcaagggt
    19141 tcaccggtga gctgcatctg ttttccgatg agcgacatct tccttatgaa cgacctccac
    19201 tctcgaaatc catgttgctg gaagattccc cgcagttaca gcaggtgtta cccgctaact
    19261 ggtggcagga aaacaatgtt catctgcatt ccggtgtaac catcaaaacg ctgggtcgcg
    19321 acacacgaga gttagtgtta accaacggcg aaagctggca ctgggatcag ctttttatag
    19381 caaccggcgc ggcagctcga ccgctgccgt tgcttgatgc actgggagaa cgctgcttta
    19441 ccctacacca tgccggtgat gccgccagac tgcgagaagt tctgcagccc gaacggtcag
    19501 tcgtgattgt cggtgccgga actattggtc tggaactggc tgccagcgcc acgcagcgca
    19561 gcgcagcgca gcgcagcgca gatgtaaggt gacagtgatt gaactggcgg caaccgtcat
    19621 gggccgtaat gcaccaccgc ccgtgcaacg ctatctttta cagcgccatc agcaggctgg
    19681 tgtgcgcatt ctgctcaata atgccattga acatgtggtc gatggtgaaa aagtagaact
    19741 gacgctgcaa agtggggaaa cgcttcaggc tgatgtggtg atttacggta ttggtatcag
    19801 cgccaacgag caactggctc gcgaggccaa ccttgatact gccaatggca ttgtcattga
    19861 tgaggcttgc cgcacctgcg atcccgcgat ctttgccggt ggcgatgtgg caatcactcg
    19921 tcttgataat ggtgcactac accgctgcga aagctgggaa aacgccaata accaggcgca
    19981 aattgccgct gccgcaatgt tagggctacc gctaccgcta ctgccgccgc cgtggttctg
    20041 gagcgatcag tacagtgata acttacagtt tattggcgat atgcgtggcg atgactggct
    20101 ttgtcgtggc aacccggaaa ctcagaaggc gatttggttt aatctgcaaa acggcgtgct
    20161 tatcggtgcg gtcacgctga atcagggccg tgagattcgc ccaattcgca aatggatcca
    20221 gagcggcaaa acgtttgatg cgaaactgct gatagatgag aacatcgcgc ttaaatcact
    20281 gtaatcagga taattagcga atatctcaat gcctggggcg tggcgaggtg caagagtgtg
    20341 tattacgttt aaatcacatt atcttgcaaa gggattggtt atgaacacac tacgttattt
    20401 tgattttgga gctgcccgcc ccgttttgtt attaattgcc cgtatcgccg tggtcttaat
    20461 tttcattatt tttggttttc ccaaaatgat gggctttgac ggtacggtcc aatatatggc
    20521 ctcgttgggc gcgccaatgc cgatgctggc agcgattatt gcggtagtta tggaagtgcc
    20581 cgccgcgata ttaatcgtgc ttggcttttt cacccgtccg ctggcggtgc tgtttatttt
    20641 ctacacgctg ggtacggcgg tgattggtca ccattactgg gatatgaccg gcgatgcggt
    20701 tgggccaaat atgattaatt tctggaagaa tgtcagtatc gctggcgcgt tcttgctatt
    20761 ggcaattacc ggaccggggg caatttctct cgatcggcgt taggaaagat gccggatgcg
    20821 gcgtgaacgc cttatccggc attttataaa ttacagccac tctacccgca acgacatctc
    20881 tgaggttgtt gattcccctg gtgctagcgc aatgagatca cccccttccg ggcgatgatg
    20941 ctcatccggc gcatggctca tcggttccag acagaagaaa tcaaacgcat atcctttatc
    21001 aaacgcaggg tcggaaacaa agatgaaata acacggtgca ggtggcgtcg tttccatgat
    21061 gatggcataa ccttcctgcg gctgctcgat gcgggcctga ccattccatc cggcaaaacc
    21121 attgttcacc cactgacgcg gcaacggcgc gagctggtta aaatccagtt cctgcggtag
    21181 ctgctcgcaa aactcacccg ccagccactg ctcccgttcc agccagtaac cgctcgcctg
    21241 cgcctgaatc cgcgtttgtg gtgacaacgg aaaataagga tgccagccgg taccaaatgg
    21301 cagcgtctct gccccttgat tggtgacaga gagcgtcacc gtcagcgtat ccgccgttaa
    21361 atgaaacgcc tgacttaccc gatagtgata gacaccgctg cgatgttcat acaccagaca
    21421 caaactatca tcgctgtgcg agacacattg ccactcgccc agccagccat cgccgtgtag
    21481 atagtgcgca tcccactcaa cattcggttg cagttgatac tcacgcccct gccagacaaa
    21541 acggttgccg ctcacccggt ttgcaaatgg caccagcgga aaacatgagg catcggttgc
    21601 cacaccgctt tttttaccag gacgtaagag cggcgtcgta tcgcgccaga agccttcgat
    21661 tacgccgccc tggtcagaaa cgtccagctt tagcgacccg tgggataagg tatagatggt
    21721 catccgctaa ctccttaatc cgggaagtta atcacaactt tcccgcattt gccgctcgcc
    21781 atcagcacat aggcatcacc tgcctgttct agcgagaagc gatgggtaat ggcgttacgc
    21841 ggccacagct tccagtcagt caaatcgtgg gcgcattttt ccatatggaa cagactggtc
    21901 acccaggagc cgataatccg ccgttgatgg tgcatcagat cagcgctgac ctcgaattcc
    21961 acttttccgg tttcaccaat gtaaaccacc cgcccccagt cagcagtgga ttgcagtgcc
    22021 agcaagcgac ctgcggcatt accggaacaa tcgagcgcaa catccgcgcc accgtgggta
    22081 agttcagcaa ttatctgcgg cagaccttcg gtggttgcta aatagccgtg atccatcccc
    22141 cctaactgtt ttgccatcgc cagacgttcc ggcagcatat caacgccgat gatccgtttt
    22201 gcaccgcgac ctttcgccag catcatcgcc atcatgccga ccggccccag accgaccacc
    22261 agcacgttat cgctgccgga aacttcgccg cgcaaaatcc cttcataggc cgtgccaacg
    22321 ccgcaactga taaacgcacc atcttcgtag ctcagcgcat ccggcaggag gatcagatct
    22381 ttttcttccg ccagcagata ttcggcatga ccgccgtcac gctgccagcc gtaagccgct
    22441 tttcctttgc cagtacaaga gataggaaag ccgcggcggc agttcgggca aaaaccacag
    22501 ccagaaatgt gatacaccag cacgcggtcg ccctctttaa aatggcggca gccttgcccc
    22561 atcgccacaa tctgcccgca cggttcatga ccgttgataa atccctggta taacggttta
    22621 tcgggtgccg ccgccgtggc acggtgttga tgatagatat agtggacatc gcttccgcaa
    22681 atcccggagg atttcatttt gatcagtacc tggttaatcc ccggcgtcgg caccgcaact
    22741 tcccgcagat cgacggttga atttcctagt aaataagcag ccagcatcgt tttcataaat
    22801 ccctcattaa caatacgatt aattttcatc cctgcagcac ccgcgccagg ggcgttaagg
    22861 tttagcgttt cgctttactg cttcgctggg tcagcaagat attcgccagc accgccacca
    22921 cgatgatgac gccgcgtacc acctgctgga aaaaggagtt aataccgagc agcaccagac
    22981 cgttaccgat tagcgtaatc accagcacac caagcaatgt accgaacagg gaaccgcgac
    23041 cgccggaaag tgccgtaccg ccgaccacga ccgcggcgat gacgtcaaac tccagaccgt
    23101 ttgcggcacc tgcgttaccg gaaccgaggc gtgccgccaa caaaatgccg gtcaccgccg
    23161 ctaataatcc cgaaagggta aagataagaa tgcgcactcg acgaacgtta ataccgcaca
    23221 actgcgccgc cgtggcatta ccgccaacgg caaaaaccga gcgcccgaag gcggtttttc
    23281 ggctaatgaa cacaaacagc gcaaacaaca ccatcatgat cagcgcggat actggcacac
    23341 cgagaaattg tccgcccagc cagtccagca cctcgttttc gtcaatcggc actggcagtg
    23401 cgttggtcat aaacagcccc attccgcgca gagcgctcca cagccccagt gtggcaacga
    23461 aacttggtac gttaaacacg ccgcgcagca ccccggcgag cgttcccatc agcgcgccta
    23521 acagcaacac cagcagacaa gcaatcgcca acggaacttc aaattgcagc aaaaatgcca
    23581 ggcacaccga gacaaaagcc accatcggcc caacgctgac atcaatttca ccggagataa
    23641 taatcagcgt catcgcccag gcggcaatcc caatggtggc ggcatcgcgc agcacgttca
    23701 tctggttatt caatgagata aagccaggcg cgttcaggga gaagaccaga taaagaatgg
    23761 caatcaccac cagcaaaccg atctcattaa tatggcgact gacaaattgt ttgagcgaga
    23821 cgctcttgcc ctgcggcaat ggtaatgacg aagcatacat cttcaggttc ctcggtaaaa
    23881 ttcatcagtg cacagacaga atggcggaca tcagctcatc cacattgacc ggcgagtgaa
    23941 actcctgcga gaacgtgccg tgctgtaata acaggatgcg gtcacacacc agcggcaatt
    24001 cctccacttc actggagata aacaccacgc tttttccttc ggcagccagc tcacggacaa
    24061 tacggtaaat ctgctgtttg gcttcgatat cgacgccgcg cgttggctcg tcgagcaaca
    24121 aaatctggct ggcggcatag acccaacgac cgatcaccac tttttgctga ttgccaccag
    24181 aaagcgtgcc gatgggtgtt tcgctactgg cagccttgac cgtcatccgc tgcatcacct
    24241 cttcggtcag gcggcggatg gtggaccatt gcagcacacc gttggtgctg attttttgcc
    24301 gattggtcag cactgtattt tcgtcaacgc ctagccaggg aatgatcccc gcttctttgc
    24361 ggttttctgg cgtatagcca atgccgcgtt tcagcatgtc gccgtaatcg gggcgcatga
    24421 ttttctcgcc gttgataaca atttcgccct gttcatacgt ctccagccca acaatcgcct
    24481 tcagcaattc actgcgccct gcccccagca ggccagcaat gccaagcact tcgccacgac
    24541 gtagcgtaaa gctgatatcc tccagcttgg gcttatggcg taacgcacgg acttccagta
    24601 cggcctgatc cacaatttcc tgaggggcaa ccggcgcaat atcaacgtga tcacgcccta
    24661 gcattagcga cacaatatga tgcgtggagg tgttttcgag catcacatcg cccgccacct
    24721 gaccatcgcg cataacggtg gcataggagg caatgcggcg gatttcttcc atccggtggc
    24781 tgacataaat caccgccacg cccagtgccg acatcttttt caccgcactg atcaccagtt
    24841 caacttctgc actggcaagc gagctggtgg gttcatcaag aatgaccacg cgcggctcgc
    24901 ccttcatcac ccgtgcaatt tccaccagtt gcttttgcgc cgggcttagc gttgaaacaa
    24961 gttgttcagg actaacgtca acgcccagcg cctgtaagca acgttgggca tcctgcgcca
    25021 tttgcaggta atcaatcatg ccgttgcggc gaggccattg accaaggcag aggttttctg
    25081 ccaccgtcag cccttccacc agacttaatt cctgataaac cgcacgaacc cccagttcag
    25141 cggcacggcg agtcaacgta gcttcgtcac cttccagtcg cgtctcgcca atccagatat
    25201 caccgctatc cgggcgttcg ctgccggtaa gcattcgaat gagagtcgat ttgcccgcgc
    25261 cgtttttacc taacagcgca cgaacttcgc ctttattgag cgtgaagtta acgttatcca
    25321 gcgcaacgac gccggggtaa cgcttatttc ctgccaccac ttttgctacc gggactgcct
    25381 ctgttgccgt gaacataggg acctctgcga atcagcgatt agggcagacc atcaacgtgc
    25441 gttgccagcc actgtttacc atcttccgtt ttggtataga gatcgattgg cacctgaatc
    25501 actttttcac cgtcggcttg tttattgata accttcaatg tttgcgcgaa aacagcattg
    25561 cccattttct taccggaaat atccactacc gctttcagca cctgattgtt ttccagctcc
    25621 tgagcaattt cggttgtcat atccgaaccg aaaacagcaa tttttccggc ctgattttga
    25681 ttacgtaccg cttttaccgc gccgagtgtc gcaccgcccg actcccccat aatggcgttg
    25741 agatccggcg tggagataat cagtttttca ccaacggaaa tcgctttatc taaaacagtc
    25801 ccttcctggt tagcgacaat ttgcgcgccg ggaacgcggg attttaatac ttcttcaaat
    25861 cctttacgtc gctgcacaca aacttcaaag gcttcgcaat tgatgacggc aattttcggc
    25921 tggtcaattt tattggcaat aaaataatcg gcggcagcgt tacccagttt ttaccaaatt
    25981 ccagtggatc gccgaccaga tacgccgaga catatttatc gacacccttt tgattaatac
    26041 aggtgttgta gcaaatcacc ggaatacccg cttcactggc gcgacgaacg gtacggctac
    26101 tgccattttc agacactgcc gataaaataa tggcatcgac attacgcgcc acgagggtat
    26161 caacaaaggt actttctttc gaaatatccc cctgggcgtt agtttcaatt aactgtacct
    26221 gtactgaaga atcttttgcc gcatcctgaa cgccctgacg cactccagcg taatatccct
    26281 gggtatcaag gtatattgca ccaatggtca tttctttatc cgcagccctg gcaaatagtg
    26341 cgctacctaa tagcgtagcc attaataata gattacgggt tgttctcatt tttttaggca
    26401 tagtgcctcc tgtagggttt ttattaacaa cggcttattc taattatttt gtgatgagcg
    26461 gcagcgcgtg ccgctcaaat attacagtta atagggttta gtgaacgtag ttaaaaataa
    26521 aaggcatcac agtggtgaat aatgccgctt tcggccaggc cggattaatc gccgtcaatt
    26581 cagcgcgagc agactcatat tgcgcaacct ccccgagtcc cgcggacgcc agcatcgcca
    26641 ccatcaggca tttttcttta tgctgctgtt gtaaatcgcc atacagcgat aacaggtcag
    26701 gttgtgaaac agcaaagaaa tccgcctcaa tactggtttt cgccatctct tgcgcccact
    26761 gtttcatttc actaaacagt tgctgtgcgg tttgctgttc acccagcagt cgcagcgcca
    26821 ttccttgcca gaagagataa tcaaccggct gatcgttgta ataactgtgg atgttaatgg
    26881 tgcgatcgcc ggtcgccgcc agacgtaaac aacgcatcgc ttcagtttca tcgccctgcg
    26941 cgttggcgca tatcgcctgc cagaaccaga tgtcgttatc agtttgcccc ggtaaacggc
    27001 cttcgcttaa attctccgga taatacagcg cggcatgaag cagttcgctg gcctgctgcg
    27061 gctctctggc atcaagatgc tgccaggcgc gtaataactg gttgaggata aactgactgg
    27121 tgaccttccc ttccccgcct tcccacgggt ggaatttacg cgtggcgaga atgtccgccg
    27181 ctttgtctgc ctgacccgtg agatgccaca aattgagcag ttctgcggtc atgtcgtcgc
    27241 gtttcagcgc aatttccaga ttattttcca gacgcgccag tcgtttctcc ggtgtggctc
    27301 cacttaactt atccagcaaa tcccgttcga aaagcagacg tgcatcctgc ggcgcaagct
    27361 gataagcatt atcaagataa cgcgcggcca gctcataatc gtgtttctta ttccacgcat
    27421 ggatcgctaa cccgcgccag ccgtcggcaa actccggcga catctctacg caacgttgcc
    27481 ataaggcaat ggctttgttg tagctacgtt tgttgtagta gaagcaggcc agtaaatggc
    27541 gagcaaacca acactcttca atactctcca gcgccgccac ttcttccagc gtattcggga
    27601 aacggacaaa ctgcgggaag acatcaatgg cttttgcgac cagttcgcca cgttcggctt
    27661 tcggcagcaa gctggcttgc aggtaaagcg gcagcgtgcg ctggcagtcc agtgcgttca
    27721 gcatctctgc cgccagggtg ggcattcccc agttaatcag ttgcccggcg gtcatcagag
    27781 cgttaacgtc gcgtccctga cacagaccgc gccactgcgc gagggctgat tcgctacgac
    27841 catcgaacca gttcagccac cacagagtgg cgttcagcgg ataatcacgc agcagtttct
    27901 cgcgctgcaa acgcgcgttg tcctgacgac cacttaacac cagcagcaga ttatgcaagc
    27961 aaagcacttc ctgattggtt gggcaggcac gaagactttg ttggcaaaaa tccagacccg
    28021 cgtcgaagtt atcattacgc gccgccagtc gtgccagacc ataatagccg cctgctttac
    28081 tattgccgct ccagaccgca cgccagaaat cctcttcggc ttgttgatat tgtccctgac
    28141 gttcgtaagc actggcgcga atcaaacttg cttgtccaca ctgcggtttt ttgttcagcg
    28201 catgtgcacg tttcagcgcc tgactggcat acgccaccgc ttgcgggaaa tctgcgcggt
    28261 tatattccag catcgccagc gccaggttac agcgataatc cagcgggtcc agcgccacgc
    28321 cgcgcaggta gtaatcgaac ggtgaacggc tggcgtgatg atattgctcc agatgctgac
    28381 cgataaacca ggcttcatct gtactggtaa tgtcttgtgc tgccagtggc gctttggcga
    28441 cgtccggcaa cggcaactcc tgcggctgat gttcctgata actcagtaca atattgccgt
    28501 cggcatcgga gagttcaatg gtcagtctgt caggattgat accgtgcaac acgccctgga
    28561 tggcggtcgc tggtgtcagg gccacggcat catcgagcaa cgcgttgcat ttgccgattt
    28621 cgcggatcgc cagacggtat ccgttcaacg gagagatggc atacagcccc cactcaatcc
    28681 cccgctcact acgctggagt tttatcaccg catcgcggga ggcattttgc accatgccca
    28741 gagaatgata aggcaggaaa tactgctcga aacgcttctc ttcgtaagca tcaagccagg
    28801 taaaatcagg ctggttatcg gcaaaaatac cggtcatcag ttcgatatac gggccgttat
    28861 tgtcggtcag actcttatcc cacgcctggc caaattcact gtgtccccaa ctccactgtt
    28921 ttttacctgg cgcaatatgg tggttggcaa cgtgcagcaa accgccatct tcatcgtgac
    28981 accacgcgcc gacaaaatcg tactgcgatt tttcggccat atatgaggtt ggaacaggca
    29041 catttttata gcgagaaatg tccactccgg cggagtagtc cactttgtag taagtgccgg
    29101 tggcgatggg gaaagcggag acggcccgtt tgccgtgatc aaacaccgca gtcacatccg
    29161 gtgggaaaac gctctgatgc ccttcacccc ctttcactgc cgggttgacc caccacaaga
    29221 aatgacgcgg cgtggcgttg ccgttataga cgcggctggc gatttccagt gccgcccggt
    29281 cagggcgcag ggtgaaacct gtcatcacct gtaaaccatg catcggctcc gtttcgccga
    29341 cccacaccgt ctgtgcaccg tcttcatggg cttcgagggt gaaatcaacg ggcataaagg
    29401 tggtcgggcg atggtgttgc ggccagttaa actcaatccc gccggagatc cacggtccca
    29461 gcatccccac cagcgcaggt ttaatgactt cattgtgata gacaaaatcg cgctgtttca
    29521 ctttatccca tgcgcgatgc acccgaccgc ccagttccgg caggatcatc actttgatgt
    29581 agtcgttttc cagccacacc gcctgctagg atttcagggt tttctgctcg ctcagcgtat
    29641 cggtcacgcc gtagggataa accgcgccgg acgatccctg ataaacgcga ttttccagga
    29701 acatgggatg tatatcctgc ggcccggttt cataggtcgg gatctcaacg cgctcttgcc
    29761 acacttttac tggagtcatg gtgccctcaa tattaacaag acatactgaa ttaaaagatt
    29821 tgtggcagtg tattgaacaa tctggcaatg ttttcgcgga ataatcacgc aattaactaa
    29881 acaaggttta gtgaagatga gagcctgcat taacaatcaa cagattcgcc accataacaa
    29941 atgcgtgatt ctggaactgc tgtaccggca aaagcgcgcc aataaatcaa cgctggcccg
    30001 gctggcgcaa atttcgattc cggcagtcag taatattttg caggaactgg aaagcgaaaa
    30061 acgggtggtg aatattgacg atgaaagcca gacgcgcggg catagtagcg gtacatggct
    30121 gattgcgccg gaaggtgact ggacgctgtg cctgaacgtg acgcccacca gtattgagtg
    30181 tcaggttgct aatgcttgtt taagtccgaa aggcgaattt gagtatttac agattgatgt
    30241 accgacgccg caggcgctgc tgtccgaaat cgaaaaatgc tggcatcgcc accgtaaatt
    30301 gtggccggac cgcaccatca atctggcgct ggcaatccac gggcaggttg atccggtgac
    30361 cggcgtatcg caaaccatgc cgcaagcgcc gtggacaacg ccagttgagg tgaagtatct
    30421 gctggaagag aagctgggca ttcgggtgct ggtcgataat gactgcgtga tgctggcgct
    30481 ggcggagaaa tggcaaaata attcgcagga acgggatttc tgcgtgatca acgttgatta
    30541 cggcattggc tcgtcgttcg tgattaacga gcaaatttat cgcggcagtt tgtatggtag
    30601 cggacagatt ggtcacacca tcgttaatcc ggatggagtc gtctgcgact gtggacgtta
    30661 cggcagcctg gaaaccgtcg cctcgttaag tgcattaaaa aaacaggcgc gggtatggct
    30721 aaaatcacaa ccggttaata ctcagcttga gcctgaaaaa ctgacaaccg cgcagttaat
    30781 cgctgcctgg cagagtggag aaccgtggat caccagctgg gttgatcgct ctgccaatgc
    30841 cattggtttg agtctgtata acttcctcaa catcctcaat attaatcaga tttggttgta
    30901 cggtcgcagt tgtgcctttg gtgagaactg gcttaatact attattcgcc agacaggatt
    30961 taacccgttc gaccgcgacg aaggaccgag cgtgaaagcg acgcaaattg gcttcgggca
    31021 attaagccgc gcacaacagg tgctgggaat tggctatttg tatgttgagg cgcagttacg
    31081 acagatttga tggcgcgata acgtagaaag gcttcccgaa ggaagccttg atgatcataa
    31141 acgaaaaatt gcctgatgcg ctacgcttat caggcctaca cggagattgc aatatattga
    31201 atttgcaaga ttttgtaggc ctgagaaggt gttcatgccg catccaacat gaacaacgag
    31261 cacattgtca gcaaatcacc gtttcgctta tgcgtaaacc gggtaacgtg cgcagatgtc
    31321 gagaacttta cctttgatgc gctcgataac ggcttcatca ttgatgctgt ccagcacgtc
    31381 acacatccag ccagccagtt ctttcgcttc ggcttcttta aagccgcgac gggtaatcgc
    31441 cggagtacct acacgaatac cggaggtcac aaacgggctc ttcggatcgt tcggtacgct
    31501 gtttttgttg acggtgatgt tagcacggcc cagagcggcg tctgcttctt taccggtcag
    31561 gtttttatca accagatcaa ccaggaacag gtggttatca gtgccgccgg aaaccacttt
    31621 gtagccgcgc tcgaggaaca cttctaccat cgctttagcg tttttcgcga cctgctgctg
    31681 gtaagttttg aactcaggct ccatcgcttc tttcagagca accgctttac cggcgattac
    31741 gtgcatcaac ggaccgccct gaccaccagg gaaaacggca gagttcagtt ttttgtacag
    31801 ctcttcgcta ccacctttcg ccaagatcag gccgccgcgc ggacccgcca gggttttgtg
    31861 agtggtggta gtaacaacgt gagcatgagg aaccgggttc gggtagacgc cagcagcaac
    31921 caggcccgca acgtgcgcca tatcaacgaa cagataagca ccgatgctgt cagcgatttc
    31981 acgcattttc gcccagtcaa ccacgccgga atatgcagag aaaccaccga taatcatttt
    32041 cggcttgtgt tctttggctt gtttttccag atcggcgtaa tcgatatgac cggtagcatc
    32101 gataccgtaa ggaacgatgt tgtacagttt accggagaag ttaaccggag aaccgtgagt
    32161 caggtgaccg ccatgcgcca ggttcatacc cagaacggta tcacctggtt ccagcagcgc
    32221 ggtgtagacc gcaaagttag cctgggagcc ggagtgcggc tggacgttag cgtagtcagc
    32281 gccgaacagt tctttcgcac gatcgatcgc cagttgttca acgatatcaa catactcgca
    32341 accgccgtag tagcgtttgc ccggataacc ttcagcatat ttgttggtca gctgagaacc
    32401 ctgcgcctgc attacgcgcg ggctggtgta gttttcggag gcgatcagtt cgatgtgctc
    32461 ttcctgacgt actttttcct gctccatagc ctgccacagt tcggcatcat aatcggcaat
    32521 gttcatttca cgctttaaca tccgcatctc ctgactcagc taacaataaa atttttggcc
    32581 tttataggcg gtcctgttgg acaacggcga acagtataac cgaatcattg tgcgataaca
    32641 ggtcttgaca aaggaattta cgcaaacgat taccttcagg ctacgcaagg ctttggagaa
    32701 taaagagctt gcaaccggaa acggatttct tttcaggttt gtgatgcaaa tttttcactt
    32761 catcacattc tttctgaaaa acaccaaaga accatttaca ttgcagggct attttttata
    32821 agatgcattt gagatacatc aattaagatg caaaaaagga agaccatatg cttgacgctc
    32881 aaaccatcgc tacagtaaaa gccaccatcc ctttactggt ggaaacgggg ccaaagttaa
    32941 ccgcccattt ctacgaccgt atgtttactc ataacccaga actcaaagaa atttttaaca
    33001 tgagtaacca gcgtaatggc gatcaacgtg aagccctgtt taacgctatt gccgcctacg
    33061 ccagtaatat tgaaaacctg cctgcgctgc tgccagcggt agaaaaaatc gcgcagaagc
    33121 acaccagctt ccagatcaaa ccggaacagt acaacatcgt cggtgaacac ctgttggcaa
    33181 cgctggacga aatgttcagc ccggggcagg aagtgctgga cgcgtggggt aaagcctatg
    33241 gtgtactggc taatgtattt atcaatcgcg aggcggaaat ctataacgaa aacgccagca
    33301 aagccggtgg ttgggaaggt actcgcgatt tccgcattgt ggctaaaaca ccgcgcagcg
    33361 cgcttatcac cagcttcgaa ctggagccgg tcgacggtgg cgcagtggca gaataccgtc
    33421 cggggcaata tctcggcgtc tggctgaagc cggaaggttt cccgcatcag gaaattcgtc
    33481 agtactcttt gactcgcaaa ccggatggca aaggctatcg tatcgcggtg aaacgcgaag
    33541 agggtgggca ggtatccaac tggttgcaca atcacgccaa tgttggcgat gtcgtgaaac
    33601 tggtcgctcc ggcaggtgat ttctttatgg ctgtcgcaga tgacacacca gtgacgttaa
    33661 tctctaccgg tgttggtcaa acgccaatgc tggcaatgct cgacacgctg gcaaaagcag
    33721 gccacacagc acaagtgaac tggttccatg cggcagaaaa tggcgaagtt cacgcctttg
    33781 ctgatgaagt taaggaactg gggcagtcac tgccgcgctt taccgcgcac acctggtatc
    33841 gtcagccgag cgaagccgat cgcgctaaag gtcagtttga tagcgaaggt ctgatggatt
    33901 tgagcaaact ggaaggtgcg ttcagcgatc cgacaatgca gttctatctc tgcggcccgg
    33961 ttggcttcat gcagtttacc gcgaaacagt tagtggatct gggcgtgaag caggaaaaca
    34021 ttcattacga atgctttggc ccgcataagg tgctgtaatt tgatgttgcc ggatggaaac
    34081 atccggcaac ccttgacgcg gtttaaattg ccgcgtcgtc ctcttcaccg gtacggatgc
    34141 gaatgacccg agccacgtca aagacgaaga ttttaccgtc accgattttg ccggtttgcg
    34201 ccgtgcgaat aatggtatcg acacaggtat cgacaatgtc gtccggtacg acaatctcaa
    34261 ttttcacttt cggcagaaaa tccaccatat actccgcgcc gcggtacagc tcggtatggc
    34321 ctttctggcg accaaagcct ttcacttcgg tcaccgtcat gccggtaata ccgacttcgg
    34381 ccagtgcttc gcggacatcg tccagcttga agggttttat aatcgcatca atctttttca
    34441 tgctattcct tgaaaaggtt gcctgtcttt tgatctgcta aacgtaacac ataacgccaa
    34501 ttcattcctt gaaatcgttt gcatccagct cgtgtcggga aagcagttta taaaattctg
    34561 tccggttgcg ccccgccatt ctcgccgcgt gggtgacgtt gcctttggtg atttgcagca
    34621 gcttacgcaa gtagttgagt tcaaaatgat tacgtgcctc aacaaaggtt ggcagcgccg
    34681 tattttcacc ctccagcgcc tgctccacca gcgcatcact aatcaccgga gatgaggtca
    34741 gcgccacgca ctgttcaatc acgttgacca actggcgcac attacccggc cagctcgcgg
    34801 tcatcaggcg tttcatcgca tcggtagaga acgcgcggac aaacggttta tgtcgctctg
    34861 ccgcctggcg caacaggtga tttgccagta gcggaatgtc ttctgtgcgc tccgccagtg
    34921 ccggaatttt caggctgaca acgttgaggc ggtaatagag atcttcgcgg aattccccgc
    34981 gcgccatcgc ttttgacaga tcacggtgag tggcagaaat aatccgcaca ttgatatcaa
    35041 tatcgcggtt actgcccagc gggcgcactt tacgctcctg caacacgcgc agcagtttga
    35101 cctgtaacgg cgcaggcata tcgccaattt catcgagaaa tagcgttccg ccttccgccg
    35161 cctggaataa accttcgcga ttgctgacag cgccagtaaa cgcgccacgc gcatgaccaa
    35221 acagctccga ctccagcaat tgctcgggca atgcgccaca gttaatagca ataaatggtt
    35281 tgctgttgcg cgggctggcg ttgtggatag cctgggcgaa aatctctttc ccggtgccgc
    35341 tctgaccgtt aatcaaaacg ctgacgtctg attgcgccac cagccgcgcc tgttccagca
    35401 aacgcagcat cagcgggctg cgggtgacaa ttgcctcgcg ccagcgttca tcggtggctg
    35461 gcgcggattg ctccagcgca tcgtcaattg cctgatatag cgcatcttta tcgacaggct
    35521 tggtgaggaa actaaaaacg ccctgctgtg ttgcagcaac ggcatcgggg atagaaccat
    35581 gcgcagtaag aataattact ggcattcccg gctgcacttt ctggatttca gcaaatagct
    35641 gcataccgtc catttcatcc atccgcaggt cgctgataac taaatctact ttttcgcgat
    35701 tcagtacccg taatccttca gcgccacttt ccgccgtgac cacactgtag ccttcgctgg
    35761 tcaggcgcag gccaagcagt ttcagcaatc ccggatcgtc atcgaccaat aataaatgcg
    35821 caggtttatg gctcatcagg agtgacctca tgggtggatg gcgcgggctt ttcactctcg
    35881 tgcggcgtat ccggcgagaa atttccagcc ggtttgcggg tcgagagctg gcgttcaata
    35941 tcggtcaggt tttccagctt gcgggtggtg agttccagtt gctgctgtag aacgtgatgt
    36001 tgctggcgca atgtatccag ctcgctgtcg ctggactgct ggagtttact gtaacgttgg
    36061 cgctcttccg ccagttgcag ttgcagcgcc tgaccgtcgc gccagagttg atacagtggg
    36121 cgaacctgtg ccgggatctc ggtacttaac gcttcaatac gcgcgaccag ctggcggcgc
    36181 tcatacggcg taattttggc gtcggcgagc aaaatccctt gtttaaaggt attttgccag
    36241 ctgccgtcgt catattggcg agcttgctga cgcgactgcg caggcattaa acgatcagca
    36301 caatccatcg cccgcagcca gtaaagcgga ttggtttcgg ttgatttacc ttgcagcgcc
    36361 cagatgtcgc tacattcagt agaaagatag tcagccagtt gataaaccgg aattttttct
    36421 tctgctggcg tatcaatggc tggcttattg tgattctgca cgcaacccag caatgccaga
    36481 catggcagcc ctgccagcca cagccgtcgg ggcaataatc gttgaaaaat gtgtcgcata
    36541 ttcaccagac ttaaagccta tcccagtggg cgtaattgtt gcagacagtc tggacatgga
    36601 cagcgcggag aaaccggggc gtacatatcg tacgtgaaga tttcgagcac tacccggggc
    36661 cgaactgaca aataaaatag cctgatagga taggctgtta gcattatttc gtgtttttcg
    36721 acgacggtaa ttcaatgcgg aaacaaacgt cttgcccgct ctcgtcgacc agatacagtt
    36781 ccccttgcat acggcgaata caatccctgg caatgcttaa tcccagaccg ctgcccttca
    36841 ccgccccttt tcgctggtgg cttccctgaa aaaagggttc gaagatcatg gcgcgttcct
    36901 cttgcggaat gggcgtgcct gtattgatga catcaatata aacccgcgca ccatgtaaac
    36961 tgctgcgaag gcaaatgtta ccggattcag ccccgtagtg caccgcattg gagtaaagat
    37021 tatccagtac gctcatcagc agcattggct ccgccaggca agctgttgct ttgagatcga
    37081 cgtcggtatg catcatttta gcccgtgcgg gcaggctatg agcagaaacc actgtctcca
    37141 ccagcggtgc taattcaaca ttctccagtt ccaccgcact gtccgcctgt ttacggttgt
    37201 aatcaagcag ttgttcgatc agtttttgca aattgcagct gctgctatca agaatgctca
    37261 ccacctcttt ttgctctggc gtaagcggcc cgacaacctg atcagccagt aattcagtgc
    37321 cctcgcgcat actcgccagt ggcgttttta attcatgaga taaatgtctt aaaaattgat
    37381 ggcgttggga ttccagccat gacaggcgct cacttaacca aagaatacgt tgcccaaccg
    37441 agcgtaactc gctcggtcca cagaacgaaa cgctattgcc cagagaacgc ccttccccca
    37501 gacggttgat catgcgctcg atatttttca ccggcccgat aatcatccgc gtgaaaagta
    37561 gcaccatcac cagactcacc agaaatagca ccagcgattg ccaaccaaaa tattgcccac
    37621 gttcggcgat ttcacgctga agttgctgcc cacgagagaa caccactgtg cgcgtggcct
    37681 gcaccatttc ggtattggca ctggcaaagg cttcaagacg cgcggcggcg gcagcatcgg
    37741 gaccactgtt gttacactga agttgggcca gattgttcaa atcctgacgc aatgcctggt
    37801 aaagtttatc gtctggcagt acgcctgcgt gggcatcgag catttcgctg taacgcttgc
    37861 gctggctttg ataaaccttc gcaagcgtag ggtcgtccag cacgcaatac tgacggtagc
    37921 tacgctccat ctccagcgcc gcgttggtca tcgcttcact acgccgggca tcaataagcg
    37981 tagtgcggtt aaccagcgcc gcctgatcgc taagcgcatt caggctttgc caggcttgcc
    38041 atgccagcac caacaggggt agtagaatta gcaaaaatgc cagcattacc agttgtcgta
    38101 atgagcgggg aaaaacgggc cagcgtttca aggtgttact ctcgtcagac gcgaatagcc
    38161 tgatgctaac cgaggggaag ttcagataca acaaagccgg gaattacccg gctttgttat
    38221 ggaataaggc ggtgcctaac tcgacgtttc gcccgatggt tgatatagct acgctgatat
    38281 cagaagttgg acggcaggca ccttgttgtg cgtcattcga attttatgta gcacgtcccg
    38341 aaggggctga cataagtcgg tgaatgagcc actgcttact attatgcagt aactgtgcca
    38401 ataaagaaaa tagtttggta acgtactgat tatacgttgc tttgagggat ttatggcttc
    38461 ttcgctattt gaatgtaaat cagccactgt gtcgctaaaa agagacaact taagataaac
    38521 ttattagatt gcaataaaat caatcattta agtgtcgcca gaaagcgaca cggtaaacca
    38581 cccattgtcg tgatttacag acacaaaaaa gctcccggag ttgggagctt atgatagtgg
    38641 ttggtgctta atgccgcatc cgggctgcaa aaccaatgaa ctgatgactt accccaactg
    38701 cttacgcgca ttgcggaaaa tgcgcatcca tgggccatcc tcgccccagt tttccggatg
    38761 ccaggagttg ctgacagtac ggaaaacacg ttccgggtgc ggcatcataa tggtgactcg
    38821 accactttca gtcgtaaccg ccgtaatacc gttcggtgaa ccgttcgggt tagccgggta
    38881 ggtttcagtg actttgccga agttatcgac atagcgcagt gccaccagcc ctttgctttc
    38941 cagtgccgcc agatgcgccg catcacgcac ttccacgcgc ccttcaccat gagagacagc
    39001 aatcggcatt tgcgagccca ccatcccctg caacagcaga gacgggcttt gggttacttc
    39061 aaccaggctg aaacgcgctt caaagcgatc ggaggtgtta cgcacaaaac gtggccacaa
    39121 ctcactcccc gggatcagtt cacgcaggtt agacatcatc tggcaaccgt tacatacccc
    39181 cagggccagc gtttgcggac gatggaagaa ggttgcaaac tcatcgcgta cacggtcatt
    39241 gaacaggata gacttcgccc aaccttcacc ggcacccagc acatcaccgt aggagaaacc
    39301 tccgcacgcg accagggcgt gaaaatcctc cagtcccgta cgtccagtca gcaggtcgct
    39361 catatgcacg tcgatagcat caaagccccc acgttggaaa gctgccgcca tttcaacatg
    39421 cgagttcacg ccctgctcac gcagtacggc aactttcgga cgtgcgccag tggcaatata
    39481 cggtgctgcc acatcttcgt tgatatcgaa cgacagtttt acattcaggc ccggatcggc
    39541 gtcgttagat ttcgcctgat gctcctgatc ggcacactcc gggttgtcac gcaggcgctg
    39601 catctgccag gtagtttctg cccaccagac acgcaacgta gtgcggcttt cgctgaatac
    39661 agtctgcccg ttggcggtaa tcacaaaacg gtcaccggaa accgcctgcc caacataatg
    39721 gacgcaatcc gcaagcccat gctgtgccag tacggactcg accgcttcac ggtcagcggc
    39781 acgaacctga atcaccgcac ccagttcttc gttaaacaac gccgccaggc gatcgtcacc
    39841 cagagaggcg atatccgcgt caatgccaca atgacctgca aaggccattt ccgccagcgt
    39901 taccagcagg cctccatcag agcggtcgtg atacgccagc agcttacgct gtgcaaccag
    39961 cgcctgaatc gcgtcataga agcctttcag ttggacaaca tcgcgtacat ctgccggttt
    40021 gtcgccaagc tgacgataaa cctgcgccag cgccgttgcg cccagcgcgt tattgccttt
    40081 gcccaaatca atcagcagca gtgcgttatc ttcggtacaa agctgcggcg tgatggtgtg
    40141 acgtacatct tccacgcggg caaatgcaga aatcaccagc gacagcggcg acgtcatttc
    40201 gcgctcttcg ttaccttcct gccagcgggt tttcatcgac atagagtctt tacccaccgg
    40261 gatcgtcagg cccagcgccg gacaaagttc ttcacccacg gctttaacgg cttcatacag
    40321 gcccgcatct tcaccagggt ggcctgccgc cgccatccag ttggcggaaa gtttaatgcg
    40381 tttgatatcg ccaatctgtg ttgcggcaat attggttaac gcttcaccga ccgccagacg
    40441 ggcagaggcg gcgaaatcca gcagcgcaac cggcgcacgc tcgccaatcg ccatcgcttc
    40501 accgtagtag ctgtcgaggc tggcggtagt gaccgcgcag ttagcgaccg gcacctgcca
    40561 cggccccacc atctgatcgc gcgccaccat gccggttacg ctgcggtcac cgatggtcac
    40621 caggaaagtt ttctccgcca cggtcggcag atgcagtacg cgtttcaccg cgtctgcaat
    40681 ggtgatccct tcacggacca gcgcatcgcc cttcgctttc agcgtttgta catcgcgggt
    40741 catcttcggc gttttaccaa gcaggacgtc cagcggcaga tcgatcggct gattatcaaa
    40801 atgacgatcg tgcagagaaa gatgcagttc ttcggtcgct tcaccaatca ccgcgtaggg
    40861 tgcgcgctca cgcttacaca gttcgtcaaa cagcggtaat tgatcggcag caaccgccag
    40921 cacgtagcgt tcctgggatt cgttacacca gatttccagc gggctcatgc ccggttcgtc
    40981 gcttagaata tcgcgcagtt caaatttacc gccgcgtccg ccgtcgctca ccagctccgg
    41041 catcgcgtta gaaagaccgc ccgcaccgac gtcgtggata aacagaattg ggttagcatc
    41101 accaagctgc cagcaacggt cgatcacttc ctggcaacga cgctccatct ccgggttgtc
    41161 gcgctgtacg gaagcaaagt cgaggtcggc atcagactga ccagacgcca tggaagacgc
    41221 cgcgccgccg ccaagaccga tgttcattgc cgggccgccg agaacgacca gcttcgcacc
    41281 gacgttgatc tcgccttttt gtacgtgatc ggcgcgaatg ttgccgatcc cgcccgccag
    41341 catgatcggt ttgtgataac cgcgcagctc ttcgccattg tggctgttca ctttttcttc
    41401 ataagtacgg aagtagccgt tcagtgccgg acgaccaaat tcgttgttaa acgccgcacc
    41461 gcccagcggg ccttcggtca tgatgtccag cgcggtaaca atgcgctcag gcttaccgaa
    41521 atcttcttcc cacggctgtt cgaagccagg aattcgcagg ttggatacgg agaaaccaac
    41581 caaaccagct ttcggcttcg cgccgcgccc ggtggcacct tcatcgcgga tttcaccgcc
    41641 ggaaccggtc gccgcgcccg gccacggaga aatcgccgtc gggtggttgt gagtttcgac
    41701 tttcatcagg atgtgcgccg gttcctgatg gaaattgtag cggcccgttt cgtggtcagc
    41761 aaagtagcgg cccacttcag aaccttccat tacggcggcg ttatctttat aagcagagag
    41821 aacgtgatct ggcgtggttt cgaaagtatt tttgatcatc ctgaacagcg atttcggctg
    41881 ttgttcaccg tcgataatcc agtcggcgtt aaaaattttg tggcggcagt gctcagagtt
    41941 cgcctgagcg aacatataca gctcgatgtc gttcgggtta cgaccaagct ttgtgaaagc
    42001 atcctgcaga tagtcaattt catcttccgc cagagccaag ccaagacgca ggttagcgtc
    42061 gatcagcgcc tgacggccct gccccagcaa atcaacgctt gtgaccggag tcggttgatg
    42121 gtgggcaaac aactgctctg catcatctaa atcaaaaaag accgtttcca tcatgcggtc
    42181 gtgcagttca gcggtaacct gctgccattg ttcattggtc agcgtaccgg cttctatata
    42241 gtaagcaacg ccgcgctcaa ggcggtttac ctgttgtagc ccgcagttat gggcaatatc
    42301 ggtcgctttc gaagaccagg gagagatggt gccaggacgc ggggtcacca gcaggagttt
    42361 gccttgcggg gcgtggctgg cgagtgccgg accatatttc agcaggcgtt caagttgtgc
    42421 gtgctcatca tcgtttaacg gcgcattgag gtcagcaaaa tggacatact cggcgtaaat
    42481 attgtgaacc gggagcctgg cagcctgaaa acgtgccagc agtttgttga ttcggaatgc
    42541 cgacagtgca ggcgaaccac gcagaatttc catcataagt ctctcgtctt ctaagctttc
    42601 ggtgtaccca agggggggga aacgggcgtc attataaaga atctgatgcg ctgacgaaac
    42661 cgtttgcgtg gaaataaaat caccatcgtg aattagcaac gcgtgccgcc aatggctgta
    42721 ataagttgcc atctggcgca ggtttacgca aaatgccgct catttatgag taaacctttc
    42781 actattatta cgttttttca agctgggacg cgcacgacac agagaattaa ctaattgaaa
    42841 aaattaaaga ttaattatct gttcatcggc attctggcac tgctgctcgc ggtcgctctc
    42901 tggccatcca ttccctggtt tggtaaagcc gacaaccgta tcgccgccat tcaagcgcgg
    42961 ggagagttgc gtgtgagcac cattcatact cccctgactt ataacgaaat caacgggaaa
    43021 ccttttggcc tggattacga actggcgaaa cagtttgccg attacctcgg cgtaaaactg
    43081 aaagtgaccg tgcggcagaa tatcagccag ctgtttgacg accttgataa tggtaacgcc
    43141 gacctgctgg cggcaggact ggtctataac agtgagcggg taaaaaatta tcagcctggc
    43201 cctacctatt attccgtgtc acaacaactg gtttataaag tgggtcagta tcgcccacgt
    43261 acgctgggca acctgacggc ggagcaactc accgttgcac cgggtcatgt ggtagttaac
    43321 gatctccaga ccctgaaaga aacaaaattc ccggaattaa gctggaaggt agacgacaaa
    43381 aaaggctctg cggaattaat ggaagatgtc atcgaaggaa aactcgatta caccattgct
    43441 gattctgtcg ccatcagcct gtttcagcgc gttcacccgg agctcgccgt agcgctcgat
    43501 atcaccgatg aacaaccggt gacttggttt agcccgttag atggcgataa taccctttcc
    43561 gccgccctgc tcgacttctt caacgaaatg aatgaagacg gtacgctggc acgcattgaa
    43621 gagaaatacc tggggcatgg cgatgatttt gattacgtcg atacgcgcac atttttacgc
    43681 gccgtcgatg cggtactgcc gcagttaaag cccctgtttg agaaatacgc cgaagaaatt
    43741 gactggcgtt tgctggccgc tattgcttat caggaatcgc actgggatgc acaggccact
    43801 tcaccgacgg gtgtgcgcgg catgatgatg ttaaccaaaa ataccgcgca aagcctcggc
    43861 attacggatc gtaccgatgc cgaacagagc atcagcggtg gcgtgcgtta tttgcaggat
    43921 atgatgagta aagtgccgga aagtgtgccg gagaacgaac ggatctggtt tgccctcgct
    43981 gcgtacaata tgggctatgc gcatatgctg gatgcccgcg ccctgacggc aaaaaccaaa
    44041 gggaatcctg acagttgggc tgacgtaaaa cagcgtctgc ctttacttag ccagaaaccc
    44101 tattacagca agctgactta cggctacgct cgtgggcatg aagcctacgc ttatgtcgaa
    44161 aatattcgta agtatcagat tagcctggtg ggttatctgc aagagaaaga gaagcaggct
    44221 acagaagcgg cgatgcaact ggcgcaggat tatccggcgg tatcgcctac ggagttgggc
    44281 aaagagaaat ttccttttct ctcgtttctt tcccagtcgt catcaaacta tttgacccat
    44341 tctccctctc tgctgttttc caggaaaggg agtgaagaga aacaaaatta atccgtcgag
    44401 gattgcgctt ttttctgcgc tttaatttcc tggcggcgca tgcgaaagaa gtcactgagc
    44461 aacgccgcgc actcatccgc cagtattcct tccgtaattt ccactcggtg attcataccc
    44521 ggatgatgca gcacatccat taaagatccc gcagcgccag ttttcgcgtc acgcgcacca
    44581 aagaccacgc gaccaatgcg actgtggatc atcgctccgg cacacattac acatggttca
    44641 agcgtgacat acaacgtggc gtcgatcaga cgataatttt gcatcaccag accaccctgc
    44701 cgcagggcca tgatttctgc atgtgcggtg ggatcatggc gaccaatcgg gcggttccag
    44761 ccttcgccga ttacccgatt gttatgcact aataccgcgc cgaccggcac ttcccgctca
    44821 tcccaggcac gtttcgccag cgtcagcgcg tgacgcatcc aatattcgtg gctaaattcg
    44881 acttcagaca aaaagaaaac tccggttata aaagcgcggc gcattataca cggacgctat
    44941 gctttactct attccagttg ctggagttca ccgcgcgggg ttactcgcca acgatgctga
    45001 cagaaataaa gcagcggatt gtcctgatta ctgtcgctat agccactgta cagccgcagc
    45061 ggcgtgccga ttttgcgctc cagttgtgcg accttttcat gtcccagaca acgcatcgtc
    45121 aatacccaac caccatagcc acgctgaatt tggctggcga taagattaac ccgcggcagc
    45181 cagggcgtat cgaaataaac cgcttcaacc agcggctgcg gagagccggt aatcaaccag
    45241 atatcagcat cggaacttaa caggtaggtg gttaatcgct cctgaaccag cggaaaggcg
    45301 gtaacattgt cgcgaaacca gcgcacgaaa tcggcctgca acgtctgtaa acgtgcttcg
    45361 ctgtgaccaa aagtgcaccc ccacagaagc agactcatcg gccagcgtgc cgcacgacct
    45421 tttatcaata acgcaatggc tataatcggt aacaacggca ggacaagtaa cgcattcagc
    45481 ggttggcgac gcagtaaata gcgcagaaaa ctgccgaaca tatcctgctg atgcaatgtt
    45541 ccatctaagt caaaaaacac cacacgacgc tcgtgagttg ccaaaccata ctcctctgga
    45601 ctgaaacatc ctgattaatt ttcgctacta catagcctaa cagatagatc atcacttttc
    45661 cggcaacaat cgaccgcatc atggctaact ggaattttta attcatggca attagcggca
    45721 atggaatata aaattcactc gcgtgtgtct catattgcct gttgtcgcca ctatttcatc
    45781 gcgaggaaag atatgaacgg cttacttcgt atccgtcagc gttaccaagg gcttgcccag
    45841 agtgataaaa aactggcgga ttatctgctg ctacaacctg atacggcgcg ccatttaagc
    45901 tctcagcaac tggccaacga agccggagtc agtcagtcca gcgtcgtgaa gttcgcgcaa
    45961 aaactcggct ataaaggttt tccggcgctt aagttggcgt tgagtgaagc gctggcaagc
    46021 cagccggaat cgccctccgt gcccattcat aaccaaatcc gcggtgatga tccgttacgg
    46081 ctggtcggcg aaaaactgat taaagaaaat accgccgcta tgtacgcaac gctaaacgtt
    46141 aatagtgaag agaaactgca tgaatgcgta acaatgttgc gctctgcgcg gcggataatt
    46201 ctgaccggta ttggcgcttc gggtctggtg gcgcaaaact ttgcctggaa gctgatgaag
    46261 attggcttca atgctgccgc actgcgcgat atgcatgcgc tactcgcaac agtacaggcg
    46321 tcgtcccctg acgatctgtt attagccatt tcctacaccg gtgtacgacg cgagttaaac
    46381 ctggcggcag atgagatgct gcgagtgggc ggaaaagtgc tggcgattac cggctttact
    46441 ccgaatgccc tgcaacagcg tgcttctcat tgcctgtata ccattgccga agaacaagcg
    46501 acaaacagtg cttcaatctc tgcttgtcac gctcagggaa tgttaacgga tttgctgttc
    46561 attgcgctga ttcagcagga tctggaactg gcaccagaac gtattcgtca tagtgaagcg
    46621 ctggtgaaaa aactggtctg agtaaagaat gcgcgtataa tgcccgcccg gtttgtgttg
    46681 ttttgagagt ttccttatgg cgctgttaat cactaaaaaa tgcatcaatt gtgatatgtg
    46741 tgaaatcaaa taaaaacaca tttattttca tagtgataca gcaatgtgtt attgaatgtg
    46801 ttgttggatt tatggtgcaa aaaaattttg cataagttca gcaacacaaa gaacaccact
    46861 ttctttaacg tcctccgcgc cctaaaggac ggggctttac ggcgcaaaag gcctgtgaca
    46921 tacaacatta acttttcagt caaccgtagc acgttcttgt atacgccgtg tctgcggttt
    46981 tatataaccc tggctggaaa cctcttatac aaagtcgatt cgccaacatc atagatgatc
    47041 gccaccttct ggcgagaaac tcctgatgca attagtcgcc cggcctgcgc ccattattct
    47101 ggtgaaagtt tgggacggcg accgccaatt cgtccctgtg cgcgagcagc ttccagtcct
    47161 gcttttgttc gttcaacaat cagttcacgt tccatttcag ccagggcacc catcacatgg
    47221 aagaaaaagc gtcccattgg tgtgctggta tcaattgaat cagtcagact acgaaagttg
    47281 atgcctcgtt cgcgcaacgc ctccaccagc acgacaagat gccgcatact gcgccccagc
    47341 cgatccagct tccagacaac cagtgtgtcg cctgccgata atgtcttgag cagttttttc
    47401 agccccggtc tttcggactt tgtaccactt atcttgtctt caaaaatcag ctcgcatcct
    47461 gcacagttca acgcattacg ttgtagatcg gtattctggt c
//
LOCUS       DAJTQQ010000025        45544 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000025 DAJTQQ010000000
VERSION     DAJTQQ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.025"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            92..2245
                     /locus_tag="OO309_001507"
     CDS             92..2245
                     /locus_tag="OO309_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5846689.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2298..4028
                     /locus_tag="OO309_001508"
     CDS             2298..4028
                     /locus_tag="OO309_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846690.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYNSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4088..4579)
                     /gene="yajQ"
                     /locus_tag="OO309_001509"
     CDS             complement(4088..4579)
                     /gene="yajQ"
                     /locus_tag="OO309_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR5846691.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4747..5658
                     /gene="panE"
                     /locus_tag="OO309_001510"
     CDS             4747..5658
                     /gene="panE"
                     /locus_tag="OO309_001510"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR5846692.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5621..6211
                     /gene="yajL"
                     /locus_tag="OO309_001511"
     CDS             5621..6211
                     /gene="yajL"
                     /locus_tag="OO309_001511"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR5846693.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6265..7713)
                     /gene="thiI"
                     /locus_tag="OO309_001512"
     CDS             complement(6265..7713)
                     /gene="thiI"
                     /locus_tag="OO309_001512"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR5846694.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7919..8161
                     /gene="xseB"
                     /locus_tag="OO309_001513"
     CDS             7919..8161
                     /gene="xseB"
                     /locus_tag="OO309_001513"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR5846695.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8161..9060
                     /gene="ispA"
                     /locus_tag="OO309_001514"
     CDS             8161..9060
                     /gene="ispA"
                     /locus_tag="OO309_001514"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR5846696.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9085..10947
                     /gene="dxs"
                     /locus_tag="OO309_001515"
     CDS             9085..10947
                     /gene="dxs"
                     /locus_tag="OO309_001515"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR5846697.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11002..11919
                     /gene="yajO"
                     /locus_tag="OO309_001516"
     CDS             11002..11919
                     /gene="yajO"
                     /locus_tag="OO309_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR5846698.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12030..12548)
                     /gene="pgpA"
                     /locus_tag="OO309_001517"
     CDS             complement(12030..12548)
                     /gene="pgpA"
                     /locus_tag="OO309_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR5846699.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12526..13503)
                     /gene="thiL"
                     /locus_tag="OO309_001518"
     CDS             complement(12526..13503)
                     /gene="thiL"
                     /locus_tag="OO309_001518"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR5846700.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13581..14000)
                     /gene="nusB"
                     /locus_tag="OO309_001519"
     CDS             complement(13581..14000)
                     /gene="nusB"
                     /locus_tag="OO309_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR5846701.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14020..14490)
                     /gene="ribE"
                     /locus_tag="OO309_001520"
     CDS             complement(14020..14490)
                     /gene="ribE"
                     /locus_tag="OO309_001520"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR5846702.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTVLEMINVLKAIKA"
     gene            complement(14579..15682)
                     /gene="ribD"
                     /locus_tag="OO309_001521"
     CDS             complement(14579..15682)
                     /gene="ribD"
                     /locus_tag="OO309_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR5846703.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15686..16135)
                     /gene="nrdR"
                     /locus_tag="OO309_001522"
     CDS             complement(15686..16135)
                     /gene="nrdR"
                     /locus_tag="OO309_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR5846704.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16286..16825
                     /locus_tag="OO309_001523"
     CDS             16286..16825
                     /locus_tag="OO309_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5846705.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17123..18007
                     /gene="tsx"
                     /locus_tag="OO309_001524"
     CDS             17123..18007
                     /gene="tsx"
                     /locus_tag="OO309_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR5846706.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18184..18531)
                     /locus_tag="OO309_001525"
     CDS             complement(18184..18531)
                     /locus_tag="OO309_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR5846707.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18660..19631)
                     /gene="secF"
                     /locus_tag="OO309_001526"
     CDS             complement(18660..19631)
                     /gene="secF"
                     /locus_tag="OO309_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR5846708.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19642..21489)
                     /gene="secD"
                     /locus_tag="OO309_001527"
     CDS             complement(19642..21489)
                     /gene="secD"
                     /locus_tag="OO309_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR5846709.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21517..21849)
                     /gene="yajC"
                     /locus_tag="OO309_001528"
     CDS             complement(21517..21849)
                     /gene="yajC"
                     /locus_tag="OO309_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR5846710.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21872..22999)
                     /gene="tgt"
                     /locus_tag="OO309_001529"
     CDS             complement(21872..22999)
                     /gene="tgt"
                     /locus_tag="OO309_001529"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR5846711.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23054..24124)
                     /gene="queA"
                     /locus_tag="OO309_001530"
     CDS             complement(23054..24124)
                     /gene="queA"
                     /locus_tag="OO309_001530"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR5846712.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24217..24790
                     /gene="acpH"
                     /locus_tag="OO309_001531"
                     /pseudo
     CDS             24217..24790
                     /gene="acpH"
                     /locus_tag="OO309_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24795..26612)
                     /gene="malZ"
                     /locus_tag="OO309_001532"
     CDS             complement(24795..26612)
                     /gene="malZ"
                     /locus_tag="OO309_001532"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR5846713.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26768..28141)
                     /gene="proY"
                     /locus_tag="OO309_001533"
     CDS             complement(26768..28141)
                     /gene="proY"
                     /locus_tag="OO309_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR5846714.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28217..29536)
                     /gene="brnQ"
                     /locus_tag="OO309_001534"
     CDS             complement(28217..29536)
                     /gene="brnQ"
                     /locus_tag="OO309_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR5846715.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29943..31238)
                     /gene="phoR"
                     /locus_tag="OO309_001535"
     CDS             complement(29943..31238)
                     /gene="phoR"
                     /locus_tag="OO309_001535"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR5846716.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31296..31985)
                     /gene="phoB"
                     /locus_tag="OO309_001536"
     CDS             complement(31296..31985)
                     /gene="phoB"
                     /locus_tag="OO309_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR5846717.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            32175..33377
                     /gene="sbcD"
                     /locus_tag="OO309_001537"
     CDS             32175..33377
                     /gene="sbcD"
                     /locus_tag="OO309_001537"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR5846718.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33374..36517
                     /gene="sbcC"
                     /locus_tag="OO309_001538"
     CDS             33374..36517
                     /gene="sbcC"
                     /locus_tag="OO309_001538"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR5846719.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36643..37824
                     /gene="araJ"
                     /locus_tag="OO309_001539"
     CDS             36643..37824
                     /gene="araJ"
                     /locus_tag="OO309_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR5846720.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37967..38875)
                     /gene="mak"
                     /locus_tag="OO309_001540"
     CDS             complement(37967..38875)
                     /gene="mak"
                     /locus_tag="OO309_001540"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR5846721.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39000..39911
                     /gene="rdgC"
                     /locus_tag="OO309_001541"
     CDS             39000..39911
                     /gene="rdgC"
                     /locus_tag="OO309_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR5846722.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(39989..40444)
                     /locus_tag="OO309_001542"
     CDS             complement(39989..40444)
                     /locus_tag="OO309_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846723.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40441..40776)
                     /locus_tag="OO309_001543"
                     /pseudo
     CDS             complement(40441..40776)
                     /locus_tag="OO309_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40931..41215)
                     /gene="ppnP"
                     /locus_tag="OO309_001544"
     CDS             complement(40931..41215)
                     /gene="ppnP"
                     /locus_tag="OO309_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR5846724.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41287..41964)
                     /locus_tag="OO309_001545"
     CDS             complement(41287..41964)
                     /locus_tag="OO309_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR5846725.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42222..42413)
                     /gene="yaiA"
                     /locus_tag="OO309_001546"
     CDS             complement(42222..42413)
                     /gene="yaiA"
                     /locus_tag="OO309_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR5846726.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(42463..42987)
                     /gene="aroL"
                     /locus_tag="OO309_001547"
     CDS             complement(42463..42987)
                     /gene="aroL"
                     /locus_tag="OO309_001547"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR5846727.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43170..43628)
                     /locus_tag="OO309_001548"
     CDS             complement(43170..43628)
                     /locus_tag="OO309_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR5846728.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43748..44557
                     /gene="proC"
                     /locus_tag="OO309_001549"
     CDS             43748..44557
                     /gene="proC"
                     /locus_tag="OO309_001549"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR5846729.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44574..>45544)
                     /gene="adrA"
                     /locus_tag="OO309_001550"
                     /gene_synonym="dgcC"
     CDS             complement(44574..>45544)
                     /gene="adrA"
                     /locus_tag="OO309_001550"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR5846730.1"
                     /translation="AGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVD
                     PLSREIYNLKTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMV
                     VSCLVTLELTGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVM
                     STRDGMTGVYNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEA
                     IVALTRQLQITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTP
                     QVTLRISVGVAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11843 a        11887 c        11137 g        10677 t
ORIGIN      
        1 cttaattcca tataactgga cgattacatt agaagtaaac tatacttttt atgaaatatc
       61 agggataaat tattcacatg gaaaatacat catgtacaaa tcattattta ctattatcat
      121 tctactgtat agcgccagtt catatgcaaa aagcgaatcc cctcccgcaa gcactataac
      181 tttcattgca caaatcgatc atgttgagct aaataaaaca gcattatctc atgatatgaa
      241 actcgttctt gccgatcgtt tttatttcaa aaccagtaat ccttgcgaaa caaattcaat
      301 ttctgctcga ccagaatcaa tggatattac tgacaaagcc tatgccgaca aattaaaaaa
      361 attagaaact ccttttcttg atgatcgcta tttctttatg accatcaatc agtgtgacgg
      421 taatggcact cccatgctga cagacataaa agtatgtaca acagaattat gtggtgctga
      481 atatacaaag aaaatgtcta ctgtatggct cgatgaaact ttaaaaccaa ctgctaaacg
      541 tctggcaatt tattttattc aatctccttt accctatgac gaggagaaaa aacaatggaa
      601 agtcaccgga tggtatatct catcaactga taatcagtct tcaactaaac agattgcatt
      661 tgaaggatat acagacacag ataacctctc cgattctaaa tttatttctt catttaaatc
      721 ctggtttgaa agtggaaaga taaaaaacat tgctaactat aatccaaaag gagaaagaga
      781 tggaaaatac gaaagttatt acaatagcaa tggtaacgtc tatgaatctc taagtttttc
      841 taatggtctt atcaacggtg agtttattac atattatgaa aatggggcca ttgaaagtaa
      901 acgtcatttt aataacgaca aaattaaaga tggtgaatgc cctcattttt atgaagatgg
      961 gaaaatcaaa cagcaacata gctatctgaa tagtaaactc gatggtcctg catatgaata
     1021 ttatcctgat ggcaaactca tgcaggaaaa tttctatcag caaagtgtgt tgattggaaa
     1081 cgataccagt tactaccaat ccggaaaaat aagctctata cacaatagaa attcccgtgg
     1141 tcagtatgat ggagctaacg aacgttatag tgaagagggt aagttactgt ctaaatccgt
     1201 ctataaagac ggaaaacaaa tttctgttca aacctggtat gaaaatgggc agaaagaaga
     1261 ggagaaacat tttgatgagc aaggccaatt aaatggtctg gttaagcaat ggtataaaaa
     1321 tggtaatctt gcaaaatcac aaaactataa acatgacata ctcgatggta acagtgaaga
     1381 gtggtacgaa aatggcatcc ctgaaagttt gtacccttat aagaatggaa aaaccgacgg
     1441 cattgccaaa tcatggaata agtatggtaa attaacctat tcaatcgaat acaaaaatgg
     1501 cgtagagaat ggggcatatc gtaactggag caaaaacaca ggtaaactaa ccaaagaaac
     1561 cctgtacgtc aatggtatca gacagggagt ggaaaaagag tttaacgatc gcacaggtaa
     1621 actattaacc tccacgcaat atgttaataa taaaagacat ggtacggagg aaacttatga
     1681 tcaaaacgga ataaaatata ttacatgcta tcaaaatgat cagaaattat cgtctttaga
     1741 caatccaaca caaataaaag ataatgcaac aacaggcgat tccagtgctc aatttgcatt
     1801 agggaaatac gaattcatct gtgcaaatat agatgaaggg attaaatggt taaccaaatc
     1861 tgcagagcaa aaaaacactg atgcgatata ctttttagct acagcctaca aaggcaatgg
     1921 tatacctgca aacaatgaaa aatacctagc atatctgcaa caagctgcaa cactgggcag
     1981 tagtaatgcc caggcggaaa ttggctattt atatctcatc ggaaaagaac taccacagaa
     2041 tcttcctgat gctggtgtct ggttcaaaaa agcagctgca caaggtaatt ttgccgcaca
     2101 tttctatctg ggaaggatgt atcagaatgg tgacggcgtt gagagaaata tggaaaaagc
     2161 caggtttcat ctatctaatg cggcagaagg tggaatagaa cctgcgctta aagcattaaa
     2221 tgaacttgaa catcaaacaa aataaagcgc tcagggacca cgtaaggttc atcaaacaga
     2281 aaaggatata gtttgcgatg aaagcgaaat ccactttgtt attaatgatt ttatctgttt
     2341 tttattctca tcaggtttta tccagcgaac ccactcagcc ggatgtttat tctgtcgtcg
     2401 agaaaaaact aaactctgct ttacctctgg aagaaaatac caccttcaga tcgcaggctg
     2461 aatggtttaa tttgcaatat gaattattag tatctggcta ccctgaacgt gcttacaatt
     2521 tactgagcaa acttgagaca gaatcgaaaa acgctaaata ttatctggat ttaactgcct
     2581 ccagagtcgc tgaagaagaa gcaccagaga cagccttaac ttttttatca aaaattgggt
     2641 taaataaacc ggctactctt acatcctttg agagctatat caatagctgg ataaaaagca
     2701 aacaacccga aaaggctatg cagcttctca gcctcgatga gaatgccctg aattattatt
     2761 taccgactgt gttagtcgca tatcagatcg ttcctgataa ggcaataagt atttataatt
     2821 ctgtatatgg agataatgtt gttataccct atcagcaagt caccatgtta cttacagttg
     2881 cggagggtta tcaaacaaaa ggcgatataa aaaacgccat tcattatgcc gataaggcgc
     2941 tgaatatgtt tgataaagct attgctgtag atagcagtcg tgaatcccat tatagtgatg
     3001 agtatttgaa actcatgaat atctacgccg ccaatggcaa caaagaaaaa gcggtggttt
     3061 tatctcaacg tctacacaaa gccattaccg aatctcaatc gaattactta agtactctgg
     3121 aaggaatttt attgttttat agaaataatg atatgcagca agactatcaa aacagccttt
     3181 caaattacat agtatttatc gataaaattt ttgccttttc cccatcaaca agaagtgaat
     3241 taagcctcat taatttatta aattcattaa atgaggtgga attgatgaga aaacgtctgg
     3301 cgtttctgat gacagcacca gaatacgctt gttataatag tcagtattgt tatgaagaca
     3361 aagtcagcgc attaaaaatc ctgtatcgtc atcatgacaa tgagctaatg gacaaatatt
     3421 tcacgactct tcttaaagag atcagtaatc aaccattaga agactgggat ataataataa
     3481 gtaatactgg tgaaaaattt agtgaagtgg gtctaaccaa atatgctcaa catctcgcat
     3541 ctttcgctga agaattttat ataaaagagc aaaaatcaca ttctgaaagt gaaatgagag
     3601 gactattttc atcacttgcc gaattatata gttttggtaa tgatactgcc agcgcaaacc
     3661 gagtgttcca tcagcatgtc cctgccttca atacagatca tatgattcaa tattttataa
     3721 atgccaaaga atggaacaat gccagggaat tattgataaa agaggaaatg ttgggtatgc
     3781 ataatctgat attgttggag aatatttgta tgcaaaaaaa tgcagaatgc atggcacaca
     3841 ttacctttac gcttaataaa ttaacaacac aaccagcaat aacaaaaata gatagcgtgg
     3901 gtaatgagca actttatcag attggcaaaa tctaccataa gttggaaatt aaacctgagc
     3961 cagagcaaca ggttttaatt cagtcgcttt atgaccgggc atcaggaagc gcctctgcaa
     4021 ctcaataaat gcatggtcaa aagggccacc acggtggccc tgacaatcag tagctatatc
     4081 cagaaagtta atcgcggaag tttttgaact ggaacggctg accgagatcg ccaccgcgca
     4141 ccatcgccat gacagcctgc aaatcatcac gagatttgcc agtcacgcgg atctcatcgc
     4201 cctgaatttg cgcctgcact ttcagtttgc tgtctttgat catcttgacg atttttttct
     4261 gagtggcgct ctcaatgccc tgtttcagtt tcgcttccac aaaccaggtt ttaccgctat
     4321 gaacgatatt ttccggtaca tccagtgaac tgccttcaat gccgcgcttc agcagcttgg
     4381 cacgcagaat atccagcaac tgattgacct ggaagtcgga ctcgctcaac actttgatgg
     4441 ttttgctggc gtcgttcagc tcaaatgagg cttcaacgtt acggaagtca aaacgggact
     4501 ccacttcgcg actcgcgtta tcgaccgcgt tacgtgcttc ctgaagatca acttcagaga
     4561 caatatcgaa agatggcatc ttttcctctc ccttcatttt tgatgcgaag cataatacct
     4621 gcaaagttaa ataaccaaca gtcctgcacc aggacgtatc ccttaattgt gcatagtcgc
     4681 aacagcatgc acaattaagg gatagcctta ttgaacaccc ggagtggttg cgggtgagga
     4741 ggaacaatga aaattaccgt attgggatgc ggtgccttag ggcaattatg gcttacagca
     4801 ctttgtaaac agggtcatga agttcagggc tggctgcgtg taccgcaacc ttattgtagt
     4861 gtgaatctgg ttgagacaga tggttcgata tttaacgaat cgctgaccgc caacgatccc
     4921 gattttctcg ccaccagcga tctgctcctg gtgacgctga aagcatggca ggtttccgat
     4981 gccgtcaaaa gcctcgcatc cacactgcct gtaactacgc caatactgtt aattcacaac
     5041 ggcatgggca ccatcgaaga attgcaaaac attcagcagc cattactgat gggcaccacc
     5101 acccatgccg cccgccgcga cggcaatgtc attattcatg tggcaaacgg aatcacgcat
     5161 atcggtccgg cacggcaaca ggatggcgat tacagttatc tggcggatat tttgcaaacc
     5221 gtgttgcctg acgttgcctg gcataacaat attcgcgccg agctgtggcg caagctggcc
     5281 gtcaactgcg tgattaatcc actgactgcc atctggaatt gcccgaacgg tgaattacgt
     5341 catcatccgc aagaaattat gcaaatatgc gaagaagtcg cggcagtgat cgaacgcgaa
     5401 gggcatcata cttcagcgga agatttgcgt gattacgtga tgcaggtgat tgatgccaca
     5461 gcagaaaata tctcgtcgat gttgcaggat atccgcgcgc tgcgccacac cgaaatcgac
     5521 tatatcaatg gttttctctt acgccgcgcc cgcgcgcatg ggattgccgt accggaaaac
     5581 acccgcctgt ttgaaatggt aaaaagaaag gagagtgaat atgagcgcat cggcactggt
     5641 ttgcctcgcc cctggtagtg aagagactga agccgtcacc acgatcaacc tcttggttcg
     5701 cggcggtatc aaagtcacca ctgccagcgt cgacagcgat ggcaacctgg caattacctg
     5761 ctcgcgcggc gtgaagctgc tggcggatgc gccgctggtc gaagtggctg atggcgaata
     5821 tgacgtgatc gtgctgcctg gtggcattaa aggcacggag tgttttcgcg atagcactct
     5881 gctggttgaa accgttaaac agttccaccg ttccgggcgt atcgtcgcgg ctatttgcgc
     5941 cgcgccagcc accgtgctgg tgccgcacga tatctttccg attggcaata tgaccggctt
     6001 cccgacgctg aaagacaaaa ttcccgctga acaatggcag gacaagcgcg tcgtctggga
     6061 tgcacgggta aaattgctga ccagccaggg gccgggtaca gctatcgact ttggtctgaa
     6121 aattatcgac ctgctggttg ggcgcgagaa agcccatgaa gtggcatcac aactggtgat
     6181 ggcggcaggg atttataatt attacgagta gtgtcggatg cggcaaacgt cgcatctgac
     6241 cagatgcgac gtaaaaacta caaattacgg gcgatatacc ttcacattgt taaagccttg
     6301 ctcgcgcaga tagagcgcct gcagacggct catcaccccg cgctcacacc acagcagcca
     6361 ggttttgttc tggtcgagat cgccaaattt ggtgctcagt ttatagaacg gcagagaaac
     6421 cacatcaatc ccttcgactt tcagtggctt atcttcctgt tcatcgacag aacggatatc
     6481 gagaatcacg tcgttcgggc cgaagccgtt gacggtttcc acttccacca cttcctgctc
     6541 ggtctgctgg gcgatttcgc ggatatcaac gttattcgct tcctcaacca ctttatcgag
     6601 aatactgaag tcgaacttct cttcttccgc ttcaatcttc gatttaaccg ctttcaccgt
     6661 cgggcttttg gagatcacgc cacaatattc cggcatcgtg cgagcaaagt cttcggtgcc
     6721 aatctggcga gccaggttga tgatgtgctc tttgtcgtaa gaaatcagcg gacgcaggat
     6781 cagcgtgtca gagacgttat caatcaggcg caggttggtc agcgtctgac tggacacctg
     6841 gccgagcgct tcgccggtga ccagcgcctg tacgccgtaa cgttcagcca ctttagatgc
     6901 ggcacgcacc atcatacgtt tgaggataac gcccatctga ccgtcgtcga ttttctcgag
     6961 aatttccccg acaaccggtt cgaaattaat ggcgacaaaa cgcacgcggt gggagctgcc
     7021 aaagcggttc cacagataat gcgccacctg acgcacgcca atttcatgcg ctgcgccgcc
     7081 gaggttaaag aagcagtaat gcacgcggca gccgcgacgc atcaacatat aactggaaac
     7141 accggagtcg aaaccaccgg aaatgagcga cagcacatct tcctgggtgc caatcgggaa
     7201 accaccaata ccttcgtagc gacctttaat cagcaggagg cgatcgtctt ccacttccag
     7261 atgaacagtt acatccgggt tagtcagctt cacgcgcgcg gattcaatat gctgatttaa
     7321 accgccgccg acgtaacgtt ccacatcaat cgagctaaaa tcatgtttgc cacggcgctt
     7381 cacgcgtacg cagaaggttt tgccttccag ctgatcgcga tactgaacca acgctttctc
     7441 aaaaatatcg tgcatgtcgg taaacggcac gtcttcgact tcgagaatat ggtggatacc
     7501 cggaatacgg gtcagggcgt cgcgaatagt cagacgctgg ttttcatctt tcgcgcgaac
     7561 ttcgatgtta tcccagtggc ggacgacagc gagcgtctca tcatagtgct ttaaaacgtt
     7621 acgaatgttc ccggtaagga tttttataaa gcgcaagcgc acagattggc ttttgatggt
     7681 gatttccggg aacaatttaa tgataaactt catggcggca atggttcgtt ggcaagcctt
     7741 aagcgacttg tatagggaaa aatacagcag cccacacctg cggctgcatc caggcgcgga
     7801 agtataccac taacatcgct ttgctgcgca catcacctta ccattgcgcg ttatttgcta
     7861 tttgccctga gtccgttacc atgacggggc gaaaaatatt gagagtcaga cattcattat
     7921 gccgaagaaa aatgaggcgc ccgccagctt tgaaaaggcg ctgagcgagc tggaacagat
     7981 tgtaacccgt ctggaaagtg gcgacctgcc gctggaagag gcgctgaacg agttcgaacg
     8041 cggcgtgcag ctggcacgtc aggggcaggc caaattacaa caagccgaac agcgcgtaca
     8101 aattctgctg tctgacaatg aagacgcctc tctaacccct tttacaccgg acaatgagta
     8161 atggactttc cgcagcaact cgaagcctgc gttaagcagg ccaaccaggc gctgagccgt
     8221 tttatcgccc cactgccctt tcagaacact cccgtggtcg aaaccatgca gtatggcgca
     8281 ttattaggtg gtaagcgcct gcgacctttc ctggtttatg ccaccggtca tatgtttggc
     8341 gttagcacaa acacgctgga cgcacccgct gctgccgtag agtgtatcca cgcttactca
     8401 ttaattcatg atgatttacc ggcgatggat gatgacgatc tgcgtcgcgg tttgccgacc
     8461 tgccatgtga agtttggcga agcaaacgcg attctcgctg gcgacgcttt acaaacgctg
     8521 gcgttctcga ttctaagcga tgccaatatg ccggaagtgt cggatcgcga cagaatttcg
     8581 atgatttctg aactggcgag cgccagcggt attgccggaa tgtgcggtgg tcaggcatta
     8641 gatttagacg cggaaggcaa acacgtacct ctggacgcgc ttgagcgtat tcatcgtcat
     8701 aaaaccggcg cattgattcg cgccgccgtt cgccttggtg cattaagcgc cggagataaa
     8761 gggcgtcgtg ctctgccagt actcgacaag tacgcagaga gcatcggcct tgccttccag
     8821 gttcaggatg acatcctgga tgtggtagga gatactgcaa cgttgggaaa acgccagggt
     8881 gccgaccagc aacttggtaa aagtacctac cctgcacttc tgggtcttga gcaagcccgg
     8941 aagaaagccc gggatctgat cgacgatgcc cgtcaggcgc tgaaacaact ggctgaacag
     9001 tcactcgata cctcggcact ggaagcgcta gcggactaca tcatccagcg taataaataa
     9061 acaataagta ttaataggcc cctgatgagt tttgatattg ccaaataccc gaccctggca
     9121 ctggtcgact ccacccagga gttacgactg ttgccgaaag agagtttacc gaaactctgc
     9181 gacgaactgc gccgctattt actcgacagc gtgagccgtt ccagcgggca cttcgcctcc
     9241 gggctgggca cggtcgaact gaccgtggcg ctgcactatg tctacaacac cccatttgac
     9301 caattgattt gggatgtggg acatcaggct tatccgcata aaattttgac cggacgccgc
     9361 gacaaaatcg gcaccatccg tcagaaaggc ggtctgcacc cgttcccgtg gcgcggcgaa
     9421 agcgaatatg acgtattaag cgtcgggcat tcatcaacct ccatcagtgc cggaattggt
     9481 attgcggttg ctgccgagaa agaaggcaaa aatcgccgca ccgtctgtgt gattggcgac
     9541 ggcgcgatta ccgctggcat ggcgtttgaa gcgatgaatc acgcgggcga tattcgtcct
     9601 gatatgctgg tggtcctcaa cgacaatgaa atgtcgattt ccgaaaatgt tggcgcgctt
     9661 aacaaccatc tggctcagct gctttccggt aagctttact cttcactgcg cgaaggcggg
     9721 aaaaaagttt tctctggcgt gccgcccatt aaagagctgc tcaaacgtac cgaagaacat
     9781 attaaaggca tggtggtgcc tggcacgttg tttgaagagc tgggatttaa ctacatcggc
     9841 ccggtggacg gtcacgatgt gctggggctt atcaccacgc taaagaacat gcgcgacctg
     9901 aaaggcccgc agttcctgca tatcatgacc aaaaaaggtc gtggttatga accggcagaa
     9961 aaagacccga tcactttcca cgccgtgcct aaatttgatc cctccagcgg ttgtttgccg
    10021 aaaagtagcg gcggtttgcc gagctattca aaaatctttg gcgactggtt gtgcgaaacg
    10081 gcagcgaaag acaacaagct gatggcgatt actccggcga tgcgtgaagg ttccggcatg
    10141 gtcgagtttt cacgtaaatt cccggatcgt tacttcgacg tggctattgc cgagcaacac
    10201 gcagtgacct ttgccgccgg tctggcgatt ggtggataca aacccattgt cgcgatctac
    10261 tccaccttcc tgcaacgcgc ctatgatcag gtgctgcatg acgtggcaat tcaaaagcta
    10321 ccggtcctgt tcgccattga ccgcgcgggc attgttggtg ctgacggtca aactcaccag
    10381 ggcgcttttg atctctctta cctgcgctgc ataccagaaa tggtcattat gaccccgagc
    10441 gatgaaaacg aatgtcgcca gatgctctat accggctatc actataacga tggcccgtcc
    10501 gcggtgcgct acccgcgcgg caacgcggtt ggcgtggaac tgacgccgct ggaaaaacta
    10561 ccaattggca aaggcattgt gaagcgtcgt ggcgagaaac tggtgatcct taactttggt
    10621 acgctgatgc cagaaacggc caaagtcgcc gaatcgctga acgctacgct ggtcgatatg
    10681 cgttttgtga aaccacttga tgaagcgtta attctggaaa tggccgccag ccatgaagcg
    10741 ctggtcaccg tagaagaaaa cgccattatg ggcggcgcag gtagcggagt gaacgaagtg
    10801 ctgatggccc atcgtaaacc agtacccgtg ctgaacattg gcctgccgga cttctttatt
    10861 ccgcaaggaa ctcaggaaga aatgcgcgcc gaactcggcc tcgatgccgc cggtatggaa
    10921 gccaaaatca aggcctggct ggcataatcc ctactccact cctgctatgc ttaagaaatt
    10981 attcatggca ggagtggcag catgcaatac aaccccttag gaaaaaccga ccttcgcgtt
    11041 tcccgacttt gcctcggctg tatgaccttt ggcgagccag atcgcggtaa tcacgcatgg
    11101 acactgccgg aagagagcag ccgtcccata attaaacgcg cgctggaagg tggcataaat
    11161 ttctttgata ccgccaatag ctattccgat ggtagcagcg aagagatcgt tggtcgcgca
    11221 ctgcgggatt tcgcccgtcg tgaagacgta gtcgttgcca ccaaagtgtt ccatcgcgtt
    11281 ggtgatttac cggaaggatt atcccgtgca caaattttgc gctctatcaa cgacagcctg
    11341 cgacgtctcg gcatggatta tgtcgatatc ctgcaaattc atcgctggga ttacaacacg
    11401 ccgatcgaag agacgctgga agccctgaac gacgtggtaa aagccgggaa agcgcgttat
    11461 atcggcgcgt catccatgca cgcttcgcag tttgctcagg cgctggaact acaaaaacag
    11521 cactgctggg cgcagtttgt cagtatgcag gatcactata atctgattta tcgcgaagaa
    11581 gagcgcgaga tgctgcctct gtgttatcag gagggcgtgg cggtgattcc gtggagcccg
    11641 ctggcgcggg ggcgactgac gcgtccatgg ggagaaacta ccgcacgact ggtgtctgat
    11701 gaggtgggga aaaatctcta taaagaaagc gatgaaaatg acgcgcagat cgcagagcgg
    11761 ttaacgggcg tcagtgaaga actcggtgca acacgagcac aagttgcgct ggcctggttg
    11821 ttaagtaaac cgggcattgc cgcaccgatt atcggaactt cgcgggaaga acagcttgat
    11881 gagctgctga acgcggtgga tatcactttg aagccataac agattgccga gctggaaacg
    11941 ccgtataaac cgcatgcggt cgtaggattt aaataagagt ccaggcctga tgagacgtga
    12001 caagcgtcac atcaggcatc ggtgcacaac tacgacagaa tacccagcgg ccagtgatga
    12061 ccgataaagt acaggatgcc tgcggaaatc accccggcga caatatcgtc gatcatgatc
    12121 cccatgccgc catgtacatt gcgatcaaac cagcggatcg gccacggctt ccacatatcc
    12181 agaatacgga aaatcacaaa cccggcggca acccactgcc agtcattggt cggcagcgcc
    12241 atgagcgtga tccacatacc aataaattcg tcccagacaa tgctgccgtg atcgtgcacg
    12301 cccatatctt tcgccgtttg atgacaaaga tagacgccga tacagatccc caacatcacc
    12361 accagcgagt agagctgcca gggtaaaaag gtcatcagat accagaaagg gatcgccgcc
    12421 agcgatccca tcgagccagg aacgattggg cttaatccac ttccgaatcc gacagcaagc
    12481 agatgccacg gattactcat cttcaggcga cttttcgcga catctttatg gcgtgacaaa
    12541 atggtcatat cctttccagt caaacgtgac aggttcgccg tcacgaataa aacaaagccc
    12601 ttcgatatcg gcggtcattt gcccgataca ggtaaacggt acgccaaggt gtccgagagc
    12661 aacatccagc gcgccacggt tcagttccgg caccgtgaaa cacaactcgt aatcttcacc
    12721 gccagagagc gcccagcgca gcgcctgttc cggttcaaca tggcgagaaa gcgcatcaga
    12781 aaacggcagc aatgccaggt caatacgtgc gccgcagtcg ctggctttca cgatatgccc
    12841 gagatcggaa atcagaccgt cagagagatc gatagctgaa tttgccagat cgcgcagtgc
    12901 ctgcccctgt aaaatacgcg gcgatggacg gagatgacgt ttgatcaagt agtcagcatc
    12961 tttagcatcg gcaacctgca aacggttttg caaaatcgcc agcccggcgg cgctatcgcc
    13021 cggtgtaccg gtcacataga tccagtcacc cggtttcgcc ccagagcgcg ttaaggctcg
    13081 tcccatcgga acaaagccgt ggatacccaa cgtcattgat aatggcccac gcgtggtatc
    13141 gccgccaatg agttgcatat cgtaataatt gagaagatca aacaaactgt cgctgaagga
    13201 ctcaagccac gcttcgtcta cgtccggtaa ggttaatgcc agcgtcagcc aggccggatc
    13261 ggcccccatc gctgccagat cgcttaggtt caccgccagt gctttataag ccagatcagc
    13321 aggatcgata tcagggagga aatggttgcc cgccaccagc gtatcagtgc tgatcgccag
    13381 ggtctgtttc tcggggatat tgagaagagc gcaatcgtcg ccgatgccca gttcgacatc
    13441 aagacgagaa cttcttacac ggtcaaaata acgggcaatc agggagaact cgccacatgc
    13501 catacgttat gcctcagcag gtaaaaaaga aaaggccggc gatcatggaa cagtcttccg
    13561 tgaatctacc ggcctggata ttactttttg ttagggcgaa tcacaggtgc tgctttatcg
    13621 agtacgccgt tgacgaactt atggctgtct tctgcgccga acgatttcgc cagttcgatc
    13681 gcttcgttaa tggccacttt gtatggcaca tcactacgtt tagacagttc gtacagcgca
    13741 atgcgcagta ctgctttttc tacctggccc agttcttcca gcaggcggga caggtagggc
    13801 ttcatcagtc cgtcgaggta tgcggtgtta gtcgccaccc cggccagcag ctcacggaag
    13861 tacaggacgt caacgtcttt tacatcctgt tcagccagga actggtattc aacatcagcg
    13921 atgtcgttct gggacaactg ccaggagtag agcgcctgga cggcacactc acgagcgcgg
    13981 cgacgagcag caggtttcac ggatttcccc ttactaattt caggccttga tggctttcaa
    14041 tacattaatc atttcaagca cggtcagtgc agcttctgca cctttgttgc cagctttggt
    14101 gccagcacgt tcgatcgctt gttcaatgct ttcagtggtc agaaccccaa aagcaaccgg
    14161 aatttcgctg tcctgggcaa catgcgccag gccgttgctt gcaccaccag cgacatattc
    14221 aaagtgggca gtgccaccac gaataaccgt acccagcgca atcaccgcgt cgtatttacc
    14281 ggttttagcc agtgcacccg ccgccagcgg cagctcatag gcaccaggca cccaaacaac
    14341 ggtaatgttt tcatctttta cctgaccgat acgtttcagt gcgtcaattg caccttccag
    14401 caggctgtca ttgataaagt tgttgaaacg cgcgatggtg atggcgacgc gagcgtccgg
    14461 ggtagcaacg ttagcttcaa taatgttcat tttcttcctt cgggttcgag tatggccccg
    14521 caggggggcg gattttagca taatatttcg tgcgctgctt ccctttcgag ccgggagatc
    14581 atgcacccac taaatgcagg caaacatccg ggcctacatg acgtatctct ttgaatttaa
    14641 attggggggc gtcggctaat ttctcaagcc ctggcagcga gcataatcca cgggcgtcgc
    14701 tgcctaatag tttaggtgcg atatagacaa tcagctcatc gactaaaccc gcctgtagca
    14761 atacgccagc gagcgttggc cccgcttcca cccagatgct gttaatctgc tgtttaccca
    14821 gttgcatcat cagtacaacc agatccagat gacctttatg ctctggaatc agcaaggtac
    14881 gcaccgtttc cggccactca cgagaatctt cctgcgtacg cgcgaaccag gtttcgccgg
    14941 gctgctgcac aatgcgatgt tccggcgtca cgcgattttg gctatcaatc acaatacgta
    15001 ctggctggcg gagattttgt tgcgggtaga gcgcctgagt ttgttcatcc agttcagacc
    15061 aacgcactgt taaggctgga tcatccgcca gcaccgtggc gctgctggtt aaaatggcat
    15121 gactttgtgc gcgctgtcgt tgtacgtcgc gccgcgcctg aggcgaagtg atccactggc
    15181 tttcgccgct cgccatcgcc gtgcggccat caagcgatgc gccaagtttt aactgaatat
    15241 aaggaaagcc ggtgcgcatc cgcttgagaa agcctttatt caattgctcg gcttcactca
    15301 tcatcagccc gtggctgacg tcaatgccag cctgttgcag acggtaaagt ccacgcccag
    15361 cgacctgcgg gttaggatct tgcatcgctg cgaccacgcg cgctacgcca gcggcgatga
    15421 gtgcgtcaca gcacggtggc gtacggccat gatggctaca gggttcgagt gtcacatacg
    15481 ccgttgcgcc tttggctttt tcacccgcca tacgcaacgc gtgtacttcg gcatgtggtt
    15541 cacccgctcg ttggtggtaa ccttcaccga caatttcgcc atctttgaca atgacgcacc
    15601 cgacattcgg gttggggtgg gtggtaaaac gtcctcgttg cgccagcttt agcgcccgcg
    15661 ccatgtaata ctcgtcctgc acggcttagt cctccaggcg cgcgatctct tcgccaaatt
    15721 ctttgatatc ttcgaaactg cggtagacag aggcaaaacg gatataggcg actttatcga
    15781 gctttttcaa ttgctccatc accagattgc caatcatctt gctcggtact tcgcgctcac
    15841 cggtggcgcg cagctgcgat ttaatatgat tgattgccat ttcaacgtaa tcggaactca
    15901 ccggacgttt ttccagcgcc cgcagcattc cgctacgcaa tttctcttca ttaaacggtt
    15961 cacgcacgtc gttgcttttt acaacacgcg gcataaccag ctccgccact tcaaaggtgg
    16021 tgaaacgttc attacacacc agacactgcc gacggcggcg tacggatgaa ccctcgccca
    16081 cgagacgaga gtcaattacc ttagtgtcca cggcgaaaca gaatgggcaa tgcatacggc
    16141 gtcctgacca ggtggttaac ggaaatctat tttaccctga actgacgata caacaaaggc
    16201 gcaacgcttt tgagataaag gatcgatgct ttcgctgctt ttaccctcta ccattaggct
    16261 aattgcgacg agaaagggac gacctatgaa tactaacgtt tttcgactgc tcctgctggg
    16321 aagcctgttc agccttagcg cctgtgtgca gcaaagtgaa gtgcgacaga tgaaacacag
    16381 cgtcagcacg ctgaaccagg agatgacgca actcaatcaa gaaacagtca aaatcactca
    16441 gcaaaacagg ctgaatgcaa aatccagcag tggggtttac cttctgcctg gagcgaaaac
    16501 accggcaaga ctggaaagcc agatcggtac tttacgtatg tcgctggtga atattacgcc
    16561 tgatacagat ggcaccaccc ttacactacg tattcagggt gagtcgaatg atcccttgcc
    16621 cgcattcagc ggaactgttg aatatgggca aattcaggga acaatagaca actttcagga
    16681 aatcaatgtg cagaatcaat tgattaatgc ccctgccagc gtcctcgccc ccagcgatgt
    16741 tgatattccg ttacaattaa aggggatttc tgtggatcag ttaggctttg tacgtatcca
    16801 cgacattcaa cctgttatgc agtaaacgta tttcgggacg attttgtgcg tcccgcaaca
    16861 tcttccccgt cattttgtta ctctgcttac atcacctgga ttgatagtaa aagtttgcaa
    16921 caagggcgaa agtcagtaca atccccgccc gaatgtgtgt aaacgtgaac gcaatcgatt
    16981 acgtaaatga tagaactgtg aaacgaaaca tatttttgtg agcaatgatt tttataatag
    17041 gctcctctgt atacgaaata tttagaaacg caatttgcgc ctttttcact cccgcaaggg
    17101 attttcaaac agtggcatac atatgaaaaa aacattactg gcagccggtg cggtactggc
    17161 gctctcttcg tcttttactg tcaacgcagc tgaaaacgac aaaccgcagt atctttccga
    17221 ctggtggcac cagagcgtta acgttgtcgg aagctatcac acccgtttcg gaccgcagat
    17281 ccgcaacgat acctaccttg agtacgaagc attcgctaaa aaagactggt tcgacttcta
    17341 tggttatgcg gatgcgccgg tattcttcgg cggtaactcc gatgctaaag gtatctggaa
    17401 ccacggttct ccgctgttta tggaaatcga accacgtttc tccatcgaca agctgaccaa
    17461 tactgacctt agcttcggtc cgttcaaaga gtggtacttc gcgaacaact acatttacga
    17521 catgggtcgt aataaagatg gtcgccagag cacctggtac atgggtctgg gtaccgatat
    17581 cgacactggc ctgccgatga gcctgtccat gaacgtctat gcgaaatacc agtggcagaa
    17641 ctatggcgca gcgaacgaaa acgagtggga cggttaccgt ttcaaaatta aatactttgt
    17701 gccgattacc gatctgtggg gcggtcagct gagctacatc ggcttcacca acttcgactg
    17761 gggttccgat ttaggggatg acagcggtaa cgcaatcaac ggtattaaga cccgtactaa
    17821 taactctatc gcttccagcc atattctggc tctgaactac gatcactggc actactctgt
    17881 cgtagctcgt tactggcacg acggtggtca gtggaacgac gatgcagaac tgaacttcgg
    17941 caacggcaac ttcaacgttc gctctaccgg ctggggtggt tacctggtag taggttacaa
    18001 cttctgatta tgaaaatgcc gggatttatt cccggcattt ctgattgtta acaacgtgtg
    18061 ctttgttcat gccggatgcg gcgtaaacgc tttgtccggc ccacaaaagc acgtaaattc
    18121 aatatattgc tgaaataacg taggcctgat aagcgtagcg catcaggcaa ttttacgttt
    18181 taatcacttc ttcttgttca tcatcgcttt caggtcagcg aatgggttgt acttcgcttc
    18241 accgacatct ttctgcgcat cttcccctgc gataacggtc gtaccatact gatccgcttc
    18301 ggtatatttc gaatgctcat gatcgtggca atagagacac aacaattccc agttactgcc
    18361 atcttccggg ttattggtat ggtcgtgatc aatgtggtga acggtaagtt cacgcaggtt
    18421 agaataaaca aactcgcggg aacagcgacc gcagacccac ggatagattt ttaatgcttt
    18481 ttcgcgatag ccgctttcta accgtgcata gttttttggg atgatagcca tgaaaagtta
    18541 cctggatgaa atgtagggtt acgcgcagtt tcccaagatg cggggatttt cgcaatctcc
    18601 atacatcaaa aaaaatcccg atcttctgac cgggattttc aacatcaacg ggaacttgat
    18661 taaggcagaa ttgacggctg atccgcccct tctttttcca ctttctgctg caacatgtgt
    18721 tcgcgcttca tacccagttt cagagccaac gcagatgcta cgtagatgga agatgcagtg
    18781 ccgatggaaa caccgataag catggtcagc gagaagcctt ccagtaccgg accaccgaag
    18841 agatacagca tcaggataac catcaaggta gtaccggatg tgatcaaggt acggtgcagc
    18901 gtctgggtca aggacacgtt aaagatttcg taaggcgtac cgcgacggat cttgcggaag
    18961 ttttcacgaa ttcggtccga taccacgata ctgtcgttaa gcgagtaacc gataaccgac
    19021 atcaacgatg ccacaatggt caggtcaatc tcgatatgga ataacgacaa aatacccagc
    19081 gtaatgataa cgtcgtgcgt cagcgcaata accacccctg ccgccagtcg ccactcaaag
    19141 cggaaaccta cgtacacgag gatagacagc agcgctgcca tcaacgccat cgcaccggtt
    19201 tgcgcaaggt cagcccccac gctcggaccg acgaactcaa tacgcttcac tgctgcattc
    19261 tgattggtgg attcgttaat caccttcaga acttggctgc ccagcacctg accgccggtt
    19321 tcgccttcag caggcggcat acggaccatg atgtcgtggc tgctaccaaa gttttgcagc
    19381 attggctctt caaaaccggc tttttgcaat gcatcacgca ttacgtcaat ttcagccggt
    19441 ttttcgagcg taatttcgat aaccgtacca ccggtgaaat ccagccccca gttaaagccg
    19501 cgcacgccca taataacgat agcagcgatt aacagcagac cagagatgcc gaaagcccag
    19561 tagtcccagc gcataaagtc atagacttta cggccgtggt ttagttgttc aacagtatat
    19621 tcctgtgcca catcgcactc ctcagattga cagcttcttg acgcgcttgc cgccatatag
    19681 caggtttacg atggcacgcg taccgacaat cgcggtaaac atcgacgtcg ccacaccgat
    19741 accggtagta atcgcgaacc ctttaattgc cccggtaccc actgcgtaca ggatgatgac
    19801 tttaatcagc gtggtgatgt tcgcatcgaa gatagaactg aatgcgccac gataaccttc
    19861 atcaattgcc tgttgaacag tacgtccgtt gctcaactct tctttaatac gttcgttgat
    19921 cagtacgttc gcatcgaccg ctaccgcaag ggttaagacg atacccgcga tacctggcat
    19981 actcagtgtt gcgcctggca acagcgacat aataccgacg atcaagatca agttggcaat
    20041 cagagcactg gtcgcaatca gaccaaactt cttatagaag atgatcatga acagaataga
    20101 aaccagcaga ccggcaaggc aagcctccag cccctgctca atgttctgca tacccagggt
    20161 cgggccaatt gtacgttctt caacaatctg aatcggcgcg atcaacgcac cggcacgcag
    20221 cagcagtgac agctgacggg cttcgttcgg gttgttgatg ccggtgatac ggaagctgtt
    20281 acccagacga gactggatgt tggcgatgtt aatcacctct tcctgtttca ccagaaccgc
    20341 acgaccattt gcatctttct taccgctgtc tttgtactcc acaaacaggg ttgccatcgg
    20401 tttgccgatg ttgtccttgg tgaagttaga cataatgttg ccaccagcgc tatcgagcga
    20461 gatgttaacc tgcggctggt tgtattcgtc ctggctggaa gtggagtcgg tgatatggtc
    20521 accagtcaga attacgcgtt tgtacagcac aaccggctga ccttcgcggg tctgtttcac
    20581 ttcagagtcg cccggtacgc gaccggatgc cgcagcggcc tgatcaacat tggtgtttac
    20641 cagacggaat tccagcgttg ccgtcgcgcc cagaatctct ttcgcacgcg cagtgtcctg
    20701 aataccgggc agttcaacaa cgatacggtc agcaccctga cgctgaacca ccggctccgc
    20761 cacgccaagt tggtttacac ggttacgcag gatattaatg ttctgctgca ccgcatattc
    20821 acgcgcttca ctcagacgag catcgctcat taccgcacgc agctggttgc tgccctggct
    20881 gctaatcacc aggtccggat ggcgcttgct cagatacgca atggcttcat cacgagcttt
    20941 agcatcgcgg aaagtgatgc tcaggccgta gttgttttct ttacgaacag tggtatacgg
    21001 gatgcccttt tcgcgcaggt cactgcgtag gctatcgata ttttgttcct ggagtttgcc
    21061 aagcgcggtg tccatatcca cttccatcag gaagtgaacg ccgccacgca ggtcaaggcc
    21121 gagcttcatc ggctcagcat gaatagctgc cagccagcgc ggcgttgccg gggcaaggtt
    21181 aagcgccacg acgtatttgt cacccataac gcccattaat gcttcacgag cgcgcaactg
    21241 ggtgtcagtg gagtcgaagc gcgcaagaat agcgccctct tccagtgcca cagacttagc
    21301 agttattttt tcttcttgta acgttttctg gacctggatc agcgtttgct cactggcggc
    21361 gactccgcgc gcaccagtga tctgaacagc cggatcctca ccaaacaggt tgggaagcgc
    21421 atacagcaga ccgatgacaa tcaccacgat cagcatgacg tacttccaca aaggataacg
    21481 gtttaacacg gcaattccct tagggaaaaa ttttaattac agcgccttca tggtgccttt
    21541 cggcaggacg gcagctacga agtcacgttt aataactact tcagtggtgt cattcagcgc
    21601 gatagcaatg tagccgtttt ccgctacttt ggttacgcga ccaaccaggc caccgttcgt
    21661 cagaacttca tcacctttgg caatggagtc catcagcttt ttgtgttctt tggtgcgctt
    21721 ctgctgtgga cgcaggatca tgaaatagaa aatcagaccg aataccacca gcatcaaaat
    21781 caaagacatc gggctacctt gcgccggtgc acccgttgcc gctaccgcat cagaaataaa
    21841 aaagctcatt aaatttccct cattattaat attaatcaac gttcaaaggt ggtacttctc
    21901 gcccctgacg ctggtaaaaa tcagttacga agctctctaa tttaccctct tcaatagcct
    21961 tgcgtaaacc cgccatcaaa cgctggtagt aacgaaggtt atgaatggtg ttgagtcgcg
    22021 cgcctaatat ttcgttgcaa cggtcgagat gatgcaagta agcgcgtgaa taattgcgac
    22081 aggtgtagca atcacactca ggatcgagtg ggccagtatc gctcttatat ttcgcattgc
    22141 ggattttcac cacgccatcg gtcacgaaca aatgaccatt tcgggcgttg cgggttggca
    22201 ttacgcagtc aaacatatcg ataccgcgac gtacgccttc aaccaggtct tccggtttac
    22261 caacgcccat caggtaacgc ggtttgtctg ccggaatttg cggacacaca tgctccagaa
    22321 tacggtgcat atctgctttc ggctcaccca cagccagacc gccgacagcg tagccatcaa
    22381 agccgatatc taccagacct ttaacagata tatcacgtaa atcttcgtaa atactgccct
    22441 gaattatacc aaataacgca tttttgttac caagactgtc aaaacgctca cggctacgct
    22501 tcgcccaacg cagagacatc tccatagagc gttttgcgta atcccagtca gcaggatacg
    22561 gcgtacactc atcaaagatc atgacgatat ccgaaccaag atcgtactga atctccatcg
    22621 acttttccgg gtcgaggaaa atcgggtcgc cgttgatcgg gttacggaag tgaacgccct
    22681 gttcggtgat tttacgaata tcaccaaggc tgaagacctg gaagccgccg gagtcggtaa
    22741 gaatcggtcc tttccactgc ataaaatcgt gcagatcgcc gtgcagtttc atgatttcct
    22801 ggcccgggcg cagccacagg tggaaggtgt tgccgaggat aatttgcgcg ccagtggctt
    22861 caacttcttc cggcgtcatc ccttttacgg tgccgtaggt gccaacaggc ataaaacaag
    22921 gcgtttccac tacgccacga tcaaagacca ggcggccacg gcgtgcgcga ccgtcggtgg
    22981 tgtccagttc aaatttcatt ttttctccac tacgtcagaa aaacagtcca acgtttaaac
    23041 cagcgccgcg gaattactcc ccgacgcgct cattaattgc ctgcggattg tacgtgataa
    23101 acatcgcatc accgtaacta aaaaagcgat atttctcttc taccgccgct ttataggcat
    23161 tcatggtgtg ttgataaccg gcaaaggccg aaaccagcat aatcagcgtc gactctggca
    23221 agtggaagtt cgtcaccagc gcatcgacca ctttgtactg gaagcccgga tagataaaga
    23281 tttgggtatc gtcaaagaac ggttcgataa gatcgttttt tgccgcctgt gccgcgcttt
    23341 ccagcgaacg tactgaagtg gtgccaaccg caatcacccg gttaccgcgc gctttcgccg
    23401 ccagtaccgc gtctaccaca tcctgcggta cttcagcgta ttccgagtgc atgatgtgat
    23461 cttcaatggt gtcgacgcgc accggctgga aggtgcccgc accaacgtgc aacgtcacaa
    23521 acgccatctc cacgcctttg gcgcgtaatt tttccagcaa aggctcgtca aaatgcagac
    23581 cagcggtcgg ggctgcaacc gcgcccggtt tttcgctata aacggtttga taaagttcgc
    23641 ggtcagcgtc ttcgtccgga cgatcgatat acggcggcag cggcatatgg ccgatgctgt
    23701 tgaggatatc cagtaccgag cgttcatcat taaattcgac ttcaaacagt gcgccgtggc
    23761 gcgcggtcat tgttgcgttg atactttcgt catcgcccag caacagttct gcgccaggtt
    23821 ttggcgcttt cgaggcgcga atatgcgcaa gaatgcgttt gtcgtcgagc atccgctcaa
    23881 ccagcacttc aatcttgccg ccgctggctt tacgcccaaa cagacgcgcc gggatcacgc
    23941 gggtattatt aaaaaccaga agatcgccgg ggttgagctt atcgagtaaa tcggtgaaag
    24001 taccgtgcgt cagcgcgccc gtcggcccgt ccagcgacag taaacgacag ctactgcgtt
    24061 caggcatggg atagtgggca atcagggatt cgggcaattc aaaggagaaa tcggtaacgc
    24121 gcatgacact gactcagact aaaataagag tcgggtagtc tagtgccggg gcgctctccc
    24181 tgcaaccttt accccccttt ggatagaata taatcgatga attttttagc tcacctgcat
    24241 ttagcccatc tcgcggaaag ctcgctttct ggcaatttac tggctgattt cgtacgcgga
    24301 aatcctgaag aaagttttcc gcccgacgtc gtggctggca ttcatatgca tcgacgtatt
    24361 gactgacaat ctgccggaag tccgcgaagc acaggagtgg tttcgtagtg aaacgcgccg
    24421 cgttgcgcct attacgctgg atgtcatgtg ggatcacttt ctttcccgcc actggtcgca
    24481 gctgtcgccg gactttccgc tacaggaatt tgtctgttat gcccgcgagc aagtgatgac
    24541 gattttgccg gactcaccgc cacgttttat caatctgaac aattacttgt ggtcagagca
    24601 gtggctggtg cgctatcgcg atatggattt catccagaac gtgttaaacg gcatggcaag
    24661 ccgccgccca cgtctggatg ccctgcgtga ctcctggtac gatttagacg ctcattatga
    24721 cgccctcgaa acccgcttct ggcagtttta tccgcggatg atggcgcagg cgtcacacaa
    24781 ggcgttataa aacgttagtt catccatacc gtagccgaaa tggcaggcaa agccagaatc
    24841 ccgtcagtca gttgcccatg cccttctttg cattgccatt gcacggcatt gagaaacggt
    24901 gacgcgggta gcaccacttc actggcctcg ccacggttga ttgccaccag tacacgttgc
    24961 tgattcagca cgcggacaaa taccactacg ttatcttccg catacagcac ctgacagccg
    25021 ccacgacgca gcgcctgact tttcttacgc agcacaatca ttcgccggta cagcgcgaat
    25081 aacgccgtat cctgcttttc cacctgccag gggaacggtt tacggcaaaa cggatcgttt
    25141 ttaccatcca gtcccacttc atcaccgtaa taaatgcacg gtacaccagg ccaggtgaac
    25201 agccagacca ccgccagcgg caggcgcgca atatcccgac cgagcagcgt tttaaatcgc
    25261 gcagtatcgt ggctatcgag ctgattaaac atacgtaatt gttgttgatg agaaagccct
    25321 gcgcggtaat tatccatcca ggccatacag gtttgggcgt caatttgctg cggatcataa
    25381 gagatgtcgg tattggcaag aaatccccac aacgggaatg tgaagccacg atagttcatg
    25441 gcagcatctt ccacatcggc ctgtaaccat tgccgtgcat cgccaaaatg ttcgccgaca
    25501 atatacgctt ccggctgggt ttctttcgcc gcttcggtga tcccggcaac gtgctgcata
    25561 ttattgcgcg ccccacccgc ctcccccagc atatgcacca catccagccg ccagccgtcc
    25621 atattccacg gcgctttcag ccagtggcgg acaatactgt cttccccgcg ataaatttca
    25681 ttcaccagac tttccgactg ataatccagc ttcgtcaagc tggcatagcc aagccagtcg
    25741 agcgccgtgc catcatcact aaacgagtac cagtcgcgcc agggcgattc agagttgtga
    25801 caagctcccc ccgtgccaag attgtgcctg tcaaaccagg catgggaatc gccactgtgg
    25861 ttaaacacgc cgtccagcac cagccgcatt cccagctgct gcgtattgtg tcgcaaacgc
    25921 agcaacgccc catcaccgcc aaactgcgga tcgacatggc gataatcctc ggtatcgtat
    25981 ttatgtacgc tgggagcttt aaacaccgga ttgagataca gcgctgtcac gccaagcttt
    26041 ttcagatacg gcagtttttc gcttatcccg tccagatcgc cgccatagaa cgttgatccg
    26101 cccgcctgcg ccgtgaccgg ttcatcccag tcacgcaaga tgatctcttg tccggctgca
    26161 tgatggtaat agacatgatc ctgttcagct tcacgaggaa gactacgcgc aaaacgatca
    26221 gggaagatct gataaaaaat ctgatccgca gcccattgtg ggccgatatc aggtacatcg
    26281 acggcaaact gctccagtcg tgccggcggc attcggctga agccctgcgg tgtaaaccaa
    26341 cgctggcgat cgtgccacag cagtttgaaa ctgtaacgcc gccggggttg tccgctggag
    26401 agatcaatcg ccgcacgcca tgcggtgacg cctggctgcg gctgactgcg ctgcttatgc
    26461 attggtactg acatttcttc gttatcgtgt tctgtacgca gcataatgcg ctgcggtggg
    26521 tcttcgcccg tcagccacag tgtaatgagc agttgatctt tgctttgttt aacaaatggg
    26581 ggcaccggca ggtgccatgc atttaacatc ataattcccc tgtgatgaaa atctgatcac
    26641 cttgccatag cctaatgcag gatcactcat catctgaagg atatttgggg gaggaggacg
    26701 ggggcggata actttcggaa tagccggatc aggatttcct cctgaaccgg cttacagaac
    26761 gcaagtatta ttggttttca gccagctgac gatcgtggcg gcgtttaaac atccagccaa
    26821 tcaacagcac aacaatccac gcgaaaccga catacagcga gatacgcgta tccgggtgat
    26881 aaccaatcaa cccgataata aagagcagga aaatcagccc gccgatggtc gttgctaccc
    26941 cacccggcac tttaaatttc agcgccttaa cttcttctgg cggcaaacgg cgacggaagg
    27001 cgatttgcga cagcaggatc ataatccaca cccataccgt ggcgaaggtt gccagcgaag
    27061 cgatcaccag gaagacgttt tccggcatga tgtagttcag atacaccgca aacagcagcg
    27121 cggtagtcat caccagcacc gtaacccacg gaataccgcg acgcgacgtt ttgctgaaaa
    27181 ttttcggtgc gctgccctgc tctgccatac cgtggagcat acggcctacg ccaaatacgt
    27241 cactgttaat tgccgacagc gaagcggtca gcacaacaaa gttaagaatg ctggcggcaa
    27301 aggtaatgcc catatgctgg aacgtcagta cgaacgggct accggcagtg ccaacctgat
    27361 tccacgggta gatagacata atgacgaaca gcgtaccgac gtagaacacc agaatacgca
    27421 tcggcacgga gttaatcgca cgcggaatcg atttctcagg atctttcgct tcaccggcgg
    27481 taatcccgat aatttcgatc ccaccgtaag caaacatcac catttgcaac gacattacca
    27541 tgccaagcca gccgttactg aagaagccgc cgttgctcca caggttatga ataccggtcg
    27601 gttgcccgcc gttgccaatc ccccagatga tgatgccgaa accggcgaca atcatgatga
    27661 tgatggtggc gactttaaag aacgagaacc agaattccag ctcaccgaag accttcacgc
    27721 tcatcaggtt tacggcgcaa atgatcagca ccacgctcag tacccaaatc cagtgcggca
    27781 ccgtcgggaa caagacaccc atatagatac caaaagcggt cacatcggcg atggcgacaa
    27841 taaggatttc aaaacagtag gtccatccgg taatgtaacc tgccagcggg ccaaggtttt
    27901 cctgcgcata acgcgagaaa gagctggcgg ccgggttatg taccgacatt tcccccagcg
    27961 cacgcataat gatatacgcc gcgataccac cgataatata ggccaacaac acgctcggac
    28021 cggccatttt gatggcgtct gccgaaccgt aaaacagccc ggtgccaatt gctgaaccca
    28081 gtgccataaa gcgtatgtgg cgggtactta gcccacgctt tagcttattc ttactttcca
    28141 tcaattccta acccatcaac acaataaaaa accacggggc atcgcagccc cgtggttaaa
    28201 acaaatgttc agtgatttag tgagcgctgg aggtcacctg acgacctgcc gcgcgatccc
    28261 agataatggc cagaaccacc atcaccactg ttggcattaa ccacgccaga ccttgttcgg
    28321 ccagcggtaa acgctgcgcc caggacggta agatatcgct gaatgcagat gctttgatcc
    28381 cgtcgagaat accaaaaagc aggctgataa acatcggcgg agcaatcacg cgggacgaat
    28441 tatgccacca tgagcgtgta aaacttaata caaccagtgc gatacacggc ggataaatag
    28501 cggtcagcac cggtacggag atctggatca gctggcttaa gccgaggtta gacaccacca
    28561 tcgagaagcc gccgaggata aacaccagcg tacgataaga gagcggtacg tactgggcaa
    28621 agaattctgc acaagcacag gtcaggccaa ccgccgtgac cagacaggcg atgaagatta
    28681 acgccgccag caggaagcta ccgccgccgc caaaggtgtg ctgaacgtaa gcatgcagaa
    28741 tagcagcgcc gtttgcagac tgatcgacca gcgacgcgct gtctgaaccc agacagaaca
    28801 gcgccaggta cagcaaagtc agaccaacac ccgccatcag gccagcccag acggtataac
    28861 gggtcagcag acgcgcttcg gtaacgccac gagaacgcgc cgcgttaaca ataacgatac
    28921 caaacaccat tgcgcccagc gtatccatgg tcagatagcc gttaacgaag ccgttagaaa
    28981 atgcagcgtt ttgataagcc tcagtcgccg tgctgataga acccgccggc cagacaatcg
    29041 cgacaacaga caggatgacc agcgcgataa ttttcagcgg cgcaaggaag ttgcccacgg
    29101 tatccagcag cttgcccgga tagagcgaaa ccagaataac gatagcgaaa tagaccaggc
    29161 tgtagataaa cagcggcagc gcggaatcac ccgtcagcgg cgcaatcccc acttcaaagg
    29221 aaacggtagc tgtacgcggc gtagcgaaaa gcggccccac cgccaggtaa caaaccgttg
    29281 ccagcagtac gccagcgact ttaccgattg gcgtgctgag gctgtcaaca ccgccgccaa
    29341 cttttgccag cgccactacc gttaataccg gcaggccaac ggcagtaatg aggaagccga
    29401 atgccgcagt ccagacgtgt tcgcctgcct gtaagccgac cattggaggg aaaataatgt
    29461 tacctgcgcc gacgaacaac gcaaatgtca taaagcccag agcgatgata tcgcgcgatc
    29521 ttaattgatg ggtcataaat catactgcct gtggatgtgg tgttgtaacg ttaaattttt
    29581 gtgccaatcc ctggggatga tacagcggta tatttaaaca attttagcgg gaaatactcc
    29641 caaccaggcg cggcgaagaa tagtttttcg atttaccgcg ttaatgcagt cagtatgaca
    29701 gcacctgaag cgcaatttaa acgcttataa cgttttaaag caaggaggga tctaaaaacc
    29761 agaggtatat agcgataaga aatcaccaac cagtataaaa cactatttcc gtgaaaaaga
    29821 catggctaat catgcgaaca aaaaatcacc aaccgttgta aaatctaacg cccgtatttt
    29881 gactgcaaag aaaataagcc agcatcggct ggcttatggc aataaaagat gacaaaggcg
    29941 gattaatcgc tgtttttggc aattaaacgt tccgggataa caaaactgaa acgtgttcct
    30001 tttcctactg tactctcaat attcaggcga ctttcgtgat gattcaccgc atgtttcacg
    30061 atcgctaacc ctaatccgct accgccggtt tgccgggaac gcgctttatc aacgcgataa
    30121 aaacgctcgg tcaggcgcgg aatatgctcc ggtgcaatgc ccggtccgtt atcttcaacg
    30181 ctaaattcgg caccgtgcgg cactcgctgc cagcgtacgg tgatatgcgt gccttccggc
    30241 gtatgattca cggcgttata gaccaggttc gaaatcgcac tgcgtagctg atcttcgttg
    30301 ccagacacct tgaggccgtt atctatctca aaggtaaatg tctgtttttt ctgactcaga
    30361 gtctgagcct cgcgctcaac aacgcgcagc atcatcggca catcaacctt ttcattgagc
    30421 aattgcgtcg gcgcggcttc aattttcgac agcgttagca actgcttcac cagcccttcc
    30481 atccgctggg tctgctcgcg catggtgtgc aacgcttttt cgcgtaccgc gccttccagc
    30541 ggctgctcat ccatcatctc caggtaaccc tgtaacacgg tcaatggcgt acgtaactca
    30601 tggctcacgt tggcaaaaaa gttacgccgc gccccttcca gttgatgcat ttgcgtgaca
    30661 tcacgcgcca ccatcagcaa ctgtttgtgg gtataaggca tgacgcgaat ttccagatgc
    30721 cgcccggtgt tgagcaccag attgagcggg cgagaaaaat cacgcgtttt cagatattgc
    30781 gtaaactccg ggtaacgcag taggttaagg atgttctgcc cgttatcttc cggccagcgc
    30841 aaaccaagaa tttgttgcgc cagaccgtta caccagaaaa taccgccctc ttccgtggtc
    30901 agcaccaccg cgtcgggcag cgactccgcg ccgctacgaa agcgtttaat cagattgccc
    30961 agttcacggc ggcgtttttt atttcgcagc tgcatctggt gtaagccgta tagtagcggt
    31021 tcccagctac cacgccccgg tggcggggtc atactgcgat ccacccacag ccaccatgaa
    31081 aggcgcaata aattccagaa atgccagata agcagtcctg ttaccgatgc cagcaaaaac
    31141 cagggcaggt aaccaaaaaa tgcacccagg atgaaagccg ggaggcagca aagtagcagc
    31201 tccagcacca gccttttcca cgacagccgt tccagcacgt aagatactcc agttaagaaa
    31261 tcataagccc tgctctgcgt ccgatgagca aggcgttaaa agcgggttga aaaacgatat
    31321 cctgtaccgc gcacggtctg caccatgcgg tcatgcccgc cgggctccag tgctttacgc
    31381 aggcgacgaa tgtggacatc gaccgtgcgg tcttccacat acacgttagt tccccagacg
    31441 tggtttaaca gctgctcgcg gctgtacacg cgctcaggat gcgtcataaa aaagtgcagc
    31501 agtttaaatt ctgtcggccc catctccagc ggctcttcgc ccgccatcac tcggtgagat
    31561 gtcgggttga gacttaatcc ctgcatctta atcacctctt ccaccgccat tggcgaaata
    31621 cggcgcatta ccgctttgat tcgcgccacc agctccttcg gcgaaaatgg cttggtgata
    31681 tagtcatccg cgccggtttc aaggccgcgc acgcgatctt cttcttcccc tctggcggtc
    31741 aacatcacca ctggaatatc ccgggtcatc gactcgcgct tgaggtgttt gatgaactgg
    31801 ataccggagc cgccaggtaa catccagtca aggagaatta aatccggcca gggttcattc
    31861 agttgattca cagcactgtc ataatcttcc gcttcgaccg gctgaaagcc attttgttcg
    31921 agcacgaagc agaccatttc gcgaattgga gcttcatctt ctacgaccag aatacgtctc
    31981 gccatgattt gccctgttgt aataaatagg ttgcgatcat taatgcgacg tcattatgcg
    32041 tcagatttat gacagattta tgaaaagctc gtcgcacata tcttcaggtt attgatttcc
    32101 gtggcgcaga aaaaagcaaa tggcacatct gtttgggtat aatcgcgacc atgctttttc
    32161 gccagggaac cgttatgcgc atccttcaca cctcagactg gcatctcggc cagaacttct
    32221 acagtaaaag ccgcgaagct gaacatcagg cttttcttga ctggctgctg gagacagcac
    32281 aaacccatca ggtggatgcg attattgttg ctggtgatgt tttcgatacc ggctcgccgc
    32341 ccagttacgc ccgcacgtta tacaaccgtt ttgttgtcaa tttacagcaa actggctgtc
    32401 atctggtggt actggcagga aaccatgact cggtcgccac gctgaatgaa tcgcgcgata
    32461 tcatggcgtt cctcaataca accgtggtcg ccagcgccgg acatgcgccg caaatcttgc
    32521 ctcgtcgcga cgggacgcca ggcgcagtgc tgtgccccat tccgttttta cgcccgcgtg
    32581 acattatttc cagccaggcg gggcttaacg gtattgaaaa acagcagcat ttactggcag
    32641 cgattaccga ttattaccaa caacactatg ccgatgcctg caaactgcgc ggcgatcagc
    32701 ctctgcccat catcgccacg ggacatttaa cgaccgtggg ttccagtaaa agtgacgccg
    32761 tgcgtgacat ttatattggc acgctggacg cgtttccggc acaaaacttt ccaccagccg
    32821 actacatcgc gctcgggcat attcaccgcg cacagattat tggcggcatg gaacatgttc
    32881 gctattgcgg ttcccccatt ccactgagtt ttgatgaatg cggtaagagt aaatatgtcc
    32941 atctggtgac attttcaaac ggcaaattag agagcgtgga aaacctgaac gtaccggtaa
    33001 ctcaacccat ggcggtgctg aaaggcgatc tggcgtcgat taccgcacag ctggaacagt
    33061 ggcgcgatgt atcgcaggag ccacctgtct ggctggatat cgaaatcact actgatgagt
    33121 atctgcatga tattcagcgc aaaatccagg cattaaccga atcattgcct gtcgaagtat
    33181 tgctggtacg tcggagtcgt gaacagcgcg agcgtgtgtt agccagccaa cagcgtgaaa
    33241 ccctcagcga actcagcgtc gaagaggtgt tcaatcgccg tctggcactg gaagacctgg
    33301 atgaatcgca gcagcaacgt ctgcagcatc ttttcaccac gacgttgcgt accctcgccg
    33361 gagaacacga agcatgaaaa ttctcagcct gcgcctgaaa aacctgaact cattaaaagg
    33421 cgaatggaag attgatttca cccgcgagcc gttcgccagc aacgggctgt ttgccattac
    33481 cggcccaacc ggtgcgggga aaaccaccct gctggacgcc atttgtctgg cgctgtatca
    33541 cgaaactccg cgtctctcta acgtttcaca atcgcaaaat gatctcatga cccgcgatac
    33601 cgctgaatgt ctggcggagg tggagtttga agtgaaaggt gaagcgtatc gcgcgttctg
    33661 gagccagaat cgggcgcgta accagccgga cggtaatttg caggtgcctc gcgtagagct
    33721 ggcgcgctgc gccgacggca aaattctcgc cgacaaagtg aaagataagc tggaactgac
    33781 agcgacgtta accgggctgg attacgggcg cttcacccgt tcgatgctgc tttcgcaggg
    33841 gcaatttgct gccttcctga atgccaaacc caaagaacgc gcggaattgc tcgaggagtt
    33901 aaccggcact gaaatctacg ggcaaatctc ggcgatggtt tttgagcagc acaaatcggc
    33961 ccgcactgag ctggagaagc tgcaagcgca ggccagcggc gtcacgttgc tcacgccgga
    34021 acaagtgcaa tcgctgacag cgagtttgca ggtacttact gacgaagaaa aacagttaat
    34081 taccgcgcag cagcaagaac aacaatcgct aaactggtta acgcgtcagg acgaattgca
    34141 gcaagaagcc agccgccgtc aacaggcctt gcaacaggcg ttagccgaag aagaaaaagc
    34201 gcaacctcaa ctggcggcgc ttagtctggc acaaccggca cgaaatcttc gtccacactg
    34261 ggaacgcatc gcagaacaca gcgcggcgct ggcgcatatt cgccagcaga ttgaagaagt
    34321 aaatactcgc ttacagagca caatggcgct tcgcgcgagc attcgccacc acgcggcgaa
    34381 gcagtcagca gaattacagc agcagcaaca aagcctgaat acctggttac aggaacacga
    34441 ccgcttccgt cagtggaaca acgaactggc gggttggcgt gcgcagttct cccaacaaac
    34501 cagcgatcgc gagcatctgc ggcaatggca gcaacagtta acccatgctg agcaaaaact
    34561 taatgcgctt gcggcgatca cgttgacgtt aaccgccgat gaagttgcta ccgccctggc
    34621 gcaacatgcc gagcaacgcc cactgcgtca gcgcctggtc gcgctgcatg ggcagattgt
    34681 tccccaacaa aaacgtctgg cgcagttaat ggtcactatc cagaatgtca ctctggaaca
    34741 gacgcaacgt aatgtcgcac ttaacgaaat gcgccagcgt tataaagaaa agacgcagca
    34801 acttgccgat gtgaaaacca tttgcgagca ggaagcgcgc atcaaaacgc tggaagccca
    34861 gcgtgcacag ttacaggcgg gtcagccttg tccactttgt ggttccacca gccacccggc
    34921 ggtcgaggcg tatcaggcgc tggagcctgg tgttaatcag tctcgattac tggcgctgga
    34981 aaacgaagtt aaaaagctcg gcgaagaagg tgcggcgctg cgcgggcaac tggatgcatt
    35041 aacgaagcag cttcagcgcg atgaaaacga agcgcaaagc ctccgacaag atgagcaagc
    35101 acttactcaa caatggcaag ccgtcacggc cagcctcaat atcaccttgc agccacagga
    35161 cgatattcaa ccgtggctgg atgcacaaga tgagcacgaa cgccagctgc ggttactcag
    35221 ccaacgacat gaattacaag ggcagattgc cgcgcataat cagcaaatta tccagtatca
    35281 acagcaaatt gaacaacgtc agcagcagct cttaacggca ttggcgggtt atgcactgac
    35341 attgccacag gaagatgaag aagagagctg gttggcgaca cgtcagcaag aagcgcagag
    35401 ctggcagcaa cgccagaacg aattaaccgc gctgcaaaac cgtattcagc agctgacacc
    35461 gattctggaa acgttgccgc aaagtgatga tctcccgcac agcgaagaaa ctgtggcgct
    35521 ggataactgg cggcaggttc atgaacaatg tctcgcatta cacagccagc agcagacgtt
    35581 acagcaacag gatgttctgg cggcgcaaag tctgcaaaaa gcccaagcgc agtttgacac
    35641 cgcgctacag gccagcgtct ttgacgatca gcaggcgttc cttgcggcgc taatggatga
    35701 acaaacacta acgcagctgg aacagctcaa gcagaatctg gaaaaccagc gccgtcaggc
    35761 gcaaacgctg gtcactcaga cagcagaaac gctggcacag catcaacaac accgacctga
    35821 cgggttggct ctcactgtga cggtggagca gattcagcaa gagttagcgc aaactcacca
    35881 aaagttgcgt gaaaacacca cgagtcaagg cgagattcgc cagcagctga agcaggatgc
    35941 agataaccgt cagcaacaac aaaccttaat gcagcaaatt gctcaaatga cgcagcaggt
    36001 tgaggactgg ggatatctga attcgctaat aggttccaaa gagggcgata aattccgcaa
    36061 gtttgcccag gggctgacgc tggataattt agtccatctc gctaatcagc aacttacccg
    36121 gctgcacgtg cgctatctgt tacagcgcaa agccagcgag gcgctggaag tcgaggttgt
    36181 tgatacctgg caggcagatg cggtacgcga tacccgtacc ctttccggcg gcgaaagttt
    36241 cctcgttagt ctggcgctgg cgctggcgct ttcggatctg gtcagccata aaacacgtat
    36301 tgactcgctg ttccttgatg aaggttttgg cacgctggat agcgaaacgc tggataccgc
    36361 ccttgatgcg ctggatgccc tgaacgccag tggcaaaacc atcggtgtga ttagccacgt
    36421 agaagcgatg aaagagcgta ttccggtgca gatcaaagtg aaaaagatca acggcctggg
    36481 ctacagcaaa ctggaaagta cgtttgcagt gaaataacta ttcagcagga taatgaatac
    36541 agaggggtga attatctctt ggccttgctg gtcgttatcc tgcaagctat cactttattg
    36601 gctacggtga ttggtagccg ttctggtggt tgtgatggtg gtatgaaaaa agtcatttta
    36661 tctttggctc tgggcacgtt tggtttgggg atggccgaat ttggcattat gagcgtgctc
    36721 acggagctgg cgcataacgt aggaatttcg attcctgctg ccgggcatat gatctcgtat
    36781 tatgcactgg tggtggtggt cggtgcgcca atcatcgcac tcttttccag ccgctactca
    36841 ctcaaacata tcttgttgtt tctggtggcg ttgtgcgtca ttggcaacgc catgttcacg
    36901 ctctcttcgt cttacctgat gctcgccatt ggtcggctgg tatccggctt tccgcatggc
    36961 gcattttttg gcgtcggagc gatcgtgtta tcaaaaatta tcaaacccgg aaaagtcacc
    37021 gccgccgtgg cggggatggt ttccgggatg acagtcgcca atttgctggg cattccgctg
    37081 ggaacgtatt taagtcagga atgctggcgt tacacctttt tattgatcgc tgtttttaat
    37141 attgcggtga tggcatcggt ctatttttgg gtgccagata ttcgcgacga ggcgaaagga
    37201 aatctgcgcg aacaatttca ctttttgcgc agcccggccc cgtggttaat tttcgccgcc
    37261 acgatgtttg gcaacgcagg tgtgtttgcc tggttcagct acgtaaagcc atacatgatg
    37321 tttatttccg gtttttcgga aacggcgatg acctttatta tgatgttagt tgggctaggg
    37381 atggtgctgg gaaatatgct aagtggcagg atttcaggac gttattcacc actgcgcatt
    37441 gcagcagtga ctgactttat cattgtactg gcactgctga tgctcttttt ctgcggcggc
    37501 atgaaaacaa cgtcgcttat ttttgctttt atttgttgcg cgggattatt tgccctttca
    37561 gcacctctgc aaatattgtt actgcaaaat gccaaaggag gagagttatt aggtgccgca
    37621 ggtgggcaaa tagcgtttaa cctcggtagc gccgtcggcg catattgcgg aggtatgatg
    37681 ctgacgctgg ggctggcata taattacgtg gcgctgcctg ccgccctgct ttcgtttgct
    37741 gcgatgtcat cgttgctgct gtatggtcgc tataagcgcc agcaagcggc ggatagtccg
    37801 gtgctggcga aaccactggg gtagattact gtcacggtgc gcgttgctta ttgccggatg
    37861 cggcgtgaac gccttatccg ccctacgcgg ttctggcaca ttttgtaggc ctgataagac
    37921 gcggcaagcg tcgcatcagg catgatgcgc caattgccta cgttttttac tcttgtggcc
    37981 ataaccacgc agcgccgcgt acgccgctgg aatcaccgtg cttcgcctta ctcaccggcg
    38041 tttcacattc gccgccgaag acaaattgtt taatcaactg cccaaccgtt tgatataaac
    38101 ggtctacatt gctcatcccg ccccccagga caatcacatc cggatcgaga atattcacga
    38161 catgtgccag cgattttgcc agccgcagct cgtagcgacg caatgccagt tccgctaccg
    38221 gatcgctttc ttcaaccagg cggataattt cactgccttt cagcgcatgt ccactcaaac
    38281 gacgataatc cgtcgcgaat cccgtgcccg aaataaaggt ttcaatacaa ccttgtttac
    38341 cgcaataaca agggacttcc tcgcgataac gcagttcgtc ttcgtccatc cacggtagcg
    38401 gattgtgtcc ccactcacct gccgtgccat tgccgccgat atgcgtccgc ccattgaatg
    38461 ccacgcccgc gccgcatccc gtgccgataa tcacggcaaa taccgtctgc gctcccgctg
    38521 ccgcgccatc tactgcttct gaaaccgcca gacagttggc atcatttgcc agccgcactt
    38581 cccgctgcaa cctcgcgctt aagtctttat cgaatggctg accgttgagc caggttgaat
    38641 tgacattctt caccacaccg gtgtagggcg aaatcgaacc tggaatgccc atacctaccg
    38701 ttccgcgctg ccctgtcgcc tgctccgcca tatcaaccaa cgtggcgatc gtttcaatag
    38761 tctgccggta atcatcacgc ggcgtgggca gacgatggcg gtacaactgc tcccctgcat
    38821 cgcccagtgc aatcacttca gttttggtgc cgcctaaatc gatacctata cgcactgtac
    38881 tctccttatt tttttcaata tcaatagcgt agagacggac aaccggattg gcaatgcaag
    38941 gccgccgaca attcgttatc atgcccgcta aatttaacga caaggccgtg gaaattatca
    39001 tgctgtggtt caaaaattta atggtttacc gtcttagccg cgagatttcg ctgcgtgcag
    39061 aagagatgga aaaacagcta gcctcgatgg catttacccc atgcggcagc caggacatgg
    39121 cgaagatggg ctgggttcct ccgatgggat cgcacagcga tgcgttaacg cacgttgcca
    39181 atggtcaaat tgttatctgc gcgcgcaaag aagaaaaaat cctcccgtct ccggtgatta
    39241 aacaggcgct ggaagcgaaa atcgccaaac tggaagcgga acaggcgcgt aagctgaaga
    39301 aaaccgaaaa agattcgctg aaagacgaag tgctgcactc tctgctgccg cgtgctttca
    39361 gccgttttag ccagacaatg atgtggctcg acacggttaa cggtttgatt atggtggact
    39421 gcgccagtgc caaaaaagcg gaagatacgc tggcattact gcgtaaaagc ctggggtcgt
    39481 taccggttgt accattgagc atggaaaacc cgattgaact gacgctgacc gaatgggttc
    39541 gctccggtag tgcggcacag ggcttccagc tgcttgatga agccgagctg aaatcgttgc
    39601 tggaagatgg cggcgtgatc cgcgcgaaga aacaagatct gaccagcgaa gagatcacca
    39661 atcacattga agccggaaaa gtggtgacta aactggcgct cgactggcag cagcgcattc
    39721 agtttgtgat gtgcgacgat ggttcgctca agcgtcttaa gttctgcgac gagctgcgcg
    39781 atcaaaacga agatatcgac cgtgaagatt tcgcccagcg ttttgatgcc gatttcatcc
    39841 tgatgactgg tgaactggca gcgttaattc aaaacctgat tgaaggatta ggtggcgaag
    39901 cacaacgtta attgctgatt ttcctttaat gccggatgcg acgcctgccg cgtcttatcc
    39961 ggcgtacgaa gccacaccag gcatataatt attcgctacg gcgagcaata atttttagcg
    40021 cagcaatatt atgcgtttta cgccgtaact gatctaatcg cctcatccct attcgcctgt
    40081 cagcagcacg cagaatattc actaaccccc atttatcgct ggtaaattgt tttgttaaca
    40141 gctccgcttt tggggtatca atatattcac gcaataaatc agaaattgaa caaatatccg
    40201 tcaggtaagg ataccagcaa gtttcaccgt gaaaattacg cactccagcg ttacctgcaa
    40261 taaagtcttt cgggtaatcc cagatcacgt ttttatccaa cgtgatccaa taacgcggta
    40321 aatcggtact tccaccattt tggctgcgca tgggataacg ggtacagtga atttgcaggt
    40381 tgatcgttgg cgtcagtaag tcatagatct cccgctgtaa cttgctccat ggacgttgtg
    40441 tcattgtttt tcctcaagcc ggtgacgcgc ttcactgcga attttccgcg ttgccccggt
    40501 tttcactaaa tcacgtaact gcgaaagatc gggttgttcg acaaataaga gtaatgatgg
    40561 atcctctttt aaccacgccg ttttcacatc ggcagccagc tgtggattat taatagccag
    40621 cgaccgatcc tgaggctcag tcaatgtcgt taacaacgac gccggggtat gtgcgttgcg
    40681 taatgccgtt tggcgcaaat gatcattgcg actttgcagc aatgcacgcc accattcatc
    40741 atctgattta tcttttatcg tagccagttc cgcaaaaaat acctggagat cttcactgat
    40801 atttatatca accttgatga attatgaata tatcggaaat tgaaaaaatc gcagcaaagg
    40861 aaatattatt atgggttaaa gagaggatat ttcagaaaaa tgccctcccg ttcaagggga
    40921 aggcgaggaa ttacagatag cggcacagat aagaggtagg ttcggcaact tgaagatgaa
    40981 actcactgtg accgggaaca ttaaacaccg aaccggcttc atacacttgc cagtcggtcg
    41041 cgtcaggcag taacacattc agcgcgccac tgattaccgt catctcttcc ggctcagcgg
    41101 tgctgaaggt gtattcgcct tcaaccataa cacccacgct ggcgcgacca gtgctgctgc
    41161 tggaaaagcc gatcgatttc actttgccgg aaaagtactc attactttga agcataaact
    41221 ggccctgtct ggtagttgaa catctacttc actatagggg ccagaggcgc tggctgtcac
    41281 gcaaaattac accagtaatt ccgcagccag ccgtgcaatc aatacgttag agagcaagac
    41341 aggaacatcg agctgttttt gcagtaaatc gcgatgacgt tggtgaaatc ctaaacaatc
    41401 cagcatgatg acatcagccc cttttgccag taattctttc ccagcatcaa tgattttttg
    41461 ttctgaatca tgaatggggt tacccaatga aaataccggc gatttctgca aaatttgcca
    41521 tttttgcgcc tgaacgggca gcatctcctc aaccggaacg ataaccccca cctgatgatc
    41581 ttcaacaata gaggaaacca gtggaggcaa tattcgcgac ggctcaagaa agatcgtatt
    41641 acgcgcagtc atactactaa tgtttgctgt actcattaat aaaatgacgt cataaccctg
    41701 attatcgagc acttcaacca caccttgcag atctcgctcc actttacgac gcgaaacatg
    41761 ggccagctgg ttgtcattta ataatgtgag aatggtgtct tcgcctgctt ctggcgcgta
    41821 ctccgccatc acttcttcac gacttaactt ccccagcagg ctatgatggg aaatattatc
    41881 ttcgtcaatg tattccgtca ggagcggcaa aacctcctgc ataggtacaa tgccgatggt
    41941 caggatcgcc aacgacgcac tcattgttca tccaccttac ttttctttcg tcgttaatta
    42001 ccgggcaagt gtgaagcacc actgctgaca cttacttgtg ctacaaatga caaaaagcgt
    42061 agcagcagac gtgcacggca taatgtaaag attccaaatg attccagtaa tggaatgtta
    42121 ttgtttaata ttctaattat tagaaaaaca taaattatga aaaatgtgac gcagatccct
    42181 tgtagtggca ctcagaatcc cttccgagca gtctgatgcg gtcactcttt gtcagggtcc
    42241 tcatagcgtt ttttcgcatc catcgcttcc tgagccgtcg gatgttcact gatcaacgag
    42301 tctggtttag gatgatcggc tctgagttgg taccaggtta ccgtctgtcc tggctttcct
    42361 ttttcaatcg tcactatata tgcttcacga ggataaggcg gtttcgttgg catcgttctt
    42421 ccttatttca cgggatgaac gttaagtata ggcgctctaa aatcaacaat tgatcgtctg
    42481 tgccagggcg ctacgaattt cagaaatcac ctggctgggt tcgtttgttg cgtcgatgat
    42541 aatatgcgca acttcgcgat atagcgcatc gcgttcttcc agcacttcct gaacttcttc
    42601 gctcagcggt tttcccgtta aggttggccg taaatcttct tccggtgcag cttgcagtcg
    42661 gttaaccagg actgatactg gcgcacacaa ataaaccacg atcccgttat tttgcatgaa
    42721 gtgacgatta aattccgtca gaataatgcc gccgcctgta gcgataacgg tggatgccgc
    42781 agttaccgct tccagcgccg ccgtttctct ggcgcgaaat cccgcccact cttccctttc
    42841 gacgatctcc gcgaccgtca tattgagctg tgattgcaac cactgatcgg tatcgacaaa
    42901 ccgacggtta agcgaatcgg caagggccat tccgaccgtt gttttaccac agccccgagg
    42961 cccgatcaga aaaagaggtt gtgtcatcgt gggttttccc caataggtcg caatgcggcg
    43021 aaagccggtg tcatgagaat agcgatcata ccatcaaact agtacaattt cgattgtaaa
    43081 gaaaaaattc cacttaaagt aaaaatctca atacatccct tactatacca ataaatattc
    43141 aagaatgaag tgtaaataat aaattacatt tagccacgac tacgttgcac ttccagccac
    43201 cacttctcca gctccgcggc aaaggcctgg cggtcacgtt gtgaaaggct atctggtccg
    43261 ccggtctgga tcccactggc acgtaaggta tccataaaat cgcgcatcgt caggcgctca
    43321 cgaatggtcg ctggcgtgta acgttcgccg cgcgaattaa gcgccgctgc gcctttctcg
    43381 atggcttcag cagccaaagg tatatctgcg gtgatcacca aatcgcccgc ttcacactgc
    43441 cggacaattt cgttatcggc aacgtcgaaa cctgccgcga cgcgcagcgt acgaataaat
    43501 cgcgatggcg gcacgcgtaa actctggttt gctaccagta ccagcggcat ctgcatacgt
    43561 tccgccgcgc gatacaaaat ctctttaatt acattgggac acgcgtcggc atccacccaa
    43621 attgtcataa agtcatcctt tgttaggtaa tcctctattg tgtcgcgctt ttgccttccg
    43681 gcatagttct gtttatgctt ctgccagcga ttatcaaaac aatgaatttc acggcaggag
    43741 tgaggcaatg gaaaagaaaa tcggttttat tggctgcggc aatatgggaa aagccattct
    43801 cggcggtctg attgccagcg gtcaggtgct tccagggcaa atctgggtat acaccccctc
    43861 cccggataaa gtcgccgccc tgcatgacca gttcggcatc aacgccgcag aatcggcgca
    43921 agaagtggcg caaatcgccg acatcatttt tgctgccgtt aaacctggca tcatgattaa
    43981 agtgcttagc gaaatcacct ccagcctgaa taaagactct ctggtcgttt ctattgctgc
    44041 aggtgtcacg ctcgaccagc ttgcccgcgc gctgggccat gaccggaaaa ttatccgcgc
    44101 catgccgaac actcccgcgc tggttaatgc cgggatgacc tccgtaacgc caaacgcgct
    44161 ggtaacccca gaagataccg ctgatgtgct gaatattttc cgctgctttg gcgaagcgga
    44221 agtaattgct gagccgatga tccacccggt ggtcggtgtg agcggttctt cgccagccta
    44281 cgtatttatg tttatcgaag cgatggccga cgccgccgtg ctgggcggga tgccacgcgc
    44341 ccaggcgtat aaatttgccg ctcaggcggt aatgggttcc gcaaaaatgg tgctggaaac
    44401 gggagaacat ccgggggcac tgaaagatat ggtctgctca ccgggaggca ccaccattga
    44461 agcggtacgc gtactggaag agaaaggctt ccgtgccgca gtgatcgaag cgatgacgaa
    44521 gtgtatggaa aaatcagaaa aactcagcaa atcctgatga ctttcgccgg acgtcaggcc
    44581 gccacttcgg tgcggttacg tccggctttc tttgctttgt aaagcgccaa atctgccgat
    44641 ttcaaccact cacgatagtg actcatttgt gggttcagcg gcgcaacccc cacactaatc
    44701 cgtaaagtta cctgtggcgt attcggcaag cgtaacgtgt ttagcccttc acgcacccgt
    44761 aacatggcgg taatggcgct ctcagctggc gtaccggaca tgattactgc aaactcatcg
    44821 ccgccaaacc gaccaatcac atcgctaccg cgcagggtaa tttgtaactg tcgggtaagc
    44881 gccacaatcg cttcatcgcc cacatcatga ccccaggtat cgttgatgct cttgaaatgg
    44941 tcgatatcga taatcagtaa cgttgcatcg cgattatgcc gccgacagtt atcaaattca
    45001 ttgcgtaaca tagtttccca atgacgtcgg ttatacacgc ccgtcatgcc gtcgcgagta
    45061 ctcatgacct gcaacctgcg tttatgttcc gccagtttgg ttgccgtctg gtagctgacc
    45121 cagccaaaca tcagaggata aacgacaata atgggaaggg agagccacca ttccagcgga
    45181 gcactattga acgacacggt aatgcccgta agctcgaggg tgacaaggca ggaaaccacc
    45241 atcaacacca gacccgcgac aaacagacgg gggccgcctg cccccatcaa attcagacac
    45301 agaatcatca acatagcggt ggaaggcagc acgtttacgc ccattacgcc tacccacatt
    45361 cccgctaata ctgcatcggt ttttaagttg taaatttccc ggctaagcgg atcgacggcc
    45421 ctgctcgcta tctgccaggc taaatgcggc cagacgaacg cccagccgac caacaccagc
    45481 caccaccagc ccggcggcgg gtgcgaaacc agcgttgaag caatcggtaa gaacatgcca
    45541 gcca
//
LOCUS       DAJTQQ010000026        42781 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000026 DAJTQQ010000000
VERSION     DAJTQQ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42781
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.026"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(79..615)
                     /gene="ssb1"
                     /locus_tag="OO309_001551"
     CDS             complement(79..615)
                     /gene="ssb1"
                     /locus_tag="OO309_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR5846731.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            869..3691
                     /gene="uvrA"
                     /locus_tag="OO309_001552"
     CDS             869..3691
                     /gene="uvrA"
                     /locus_tag="OO309_001552"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR5846732.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3726..4082)
                     /locus_tag="OO309_001553"
     CDS             complement(3726..4082)
                     /locus_tag="OO309_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5846733.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4086..4502)
                     /locus_tag="OO309_001554"
     CDS             complement(4086..4502)
                     /locus_tag="OO309_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR5846734.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4613..5326)
                     /gene="aphA"
                     /locus_tag="OO309_001555"
     CDS             complement(4613..5326)
                     /gene="aphA"
                     /locus_tag="OO309_001555"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR5846735.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5728..5932
                     /locus_tag="OO309_001556"
                     /pseudo
     CDS             5728..5932
                     /locus_tag="OO309_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6435..7628)
                     /gene="tyrB"
                     /locus_tag="OO309_001557"
     CDS             complement(6435..7628)
                     /gene="tyrB"
                     /locus_tag="OO309_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR5846736.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7881..8960)
                     /gene="alr"
                     /locus_tag="OO309_001558"
     CDS             complement(7881..8960)
                     /gene="alr"
                     /locus_tag="OO309_001558"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR5846737.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9013..10428)
                     /gene="dnaB"
                     /locus_tag="OO309_001559"
     CDS             complement(9013..10428)
                     /gene="dnaB"
                     /locus_tag="OO309_001559"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5846738.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10511..11494
                     /locus_tag="OO309_001560"
     CDS             10511..11494
                     /locus_tag="OO309_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR5846739.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11660..11902)
                     /gene="pspG"
                     /locus_tag="OO309_001561"
     CDS             complement(11660..11902)
                     /gene="pspG"
                     /locus_tag="OO309_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR5846740.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12036..13073)
                     /gene="dusA"
                     /locus_tag="OO309_001562"
     CDS             complement(12036..13073)
                     /gene="dusA"
                     /locus_tag="OO309_001562"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR5846741.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13436..14421)
                     /locus_tag="OO309_001563"
                     /pseudo
     CDS             complement(13436..14421)
                     /locus_tag="OO309_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14739..15254
                     /gene="zur"
                     /locus_tag="OO309_001564"
     CDS             14739..15254
                     /gene="zur"
                     /locus_tag="OO309_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR5846742.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15296..15505)
                     /locus_tag="OO309_001565"
     CDS             complement(15296..15505)
                     /locus_tag="OO309_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR5846743.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15621..16946)
                     /gene="dinF"
                     /locus_tag="OO309_001566"
     CDS             complement(15621..16946)
                     /gene="dinF"
                     /locus_tag="OO309_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR5846744.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17019..17627)
                     /gene="lexA"
                     /locus_tag="OO309_001567"
     CDS             complement(17019..17627)
                     /gene="lexA"
                     /locus_tag="OO309_001567"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR5846745.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17737..18105)
                     /gene="dgkA"
                     /locus_tag="OO309_001568"
     CDS             complement(17737..18105)
                     /gene="dgkA"
                     /locus_tag="OO309_001568"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR5846746.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18276..20699
                     /gene="plsB"
                     /locus_tag="OO309_001569"
     CDS             18276..20699
                     /gene="plsB"
                     /locus_tag="OO309_001569"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR5846747.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20854..21726)
                     /gene="ubiA"
                     /locus_tag="OO309_001570"
     CDS             complement(20854..21726)
                     /gene="ubiA"
                     /locus_tag="OO309_001570"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR5846748.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21739..22236)
                     /gene="ubiC"
                     /locus_tag="OO309_001571"
     CDS             complement(21739..22236)
                     /gene="ubiC"
                     /locus_tag="OO309_001571"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR5846749.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22459..24039)
                     /locus_tag="OO309_001572"
                     /pseudo
     CDS             complement(22459..24039)
                     /locus_tag="OO309_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24268..25188)
                     /gene="malM"
                     /locus_tag="OO309_001573"
     CDS             complement(24268..25188)
                     /gene="malM"
                     /locus_tag="OO309_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR5846750.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25340..26680)
                     /gene="lamB"
                     /locus_tag="OO309_001574"
     CDS             complement(25340..26680)
                     /gene="lamB"
                     /locus_tag="OO309_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR5846751.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26752..27867)
                     /gene="malK"
                     /locus_tag="OO309_001575"
     CDS             complement(26752..27867)
                     /gene="malK"
                     /locus_tag="OO309_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR5846752.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28232..29422
                     /gene="malE"
                     /locus_tag="OO309_001576"
     CDS             28232..29422
                     /gene="malE"
                     /locus_tag="OO309_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR5846753.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29576..31120
                     /gene="malF"
                     /locus_tag="OO309_001577"
     CDS             29576..31120
                     /gene="malF"
                     /locus_tag="OO309_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR5846754.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31135..32025
                     /gene="malG"
                     /locus_tag="OO309_001578"
     CDS             31135..32025
                     /gene="malG"
                     /locus_tag="OO309_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR5846755.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPISI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            32396..33871
                     /gene="xylE"
                     /locus_tag="OO309_001579"
     CDS             32396..33871
                     /gene="xylE"
                     /locus_tag="OO309_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR5846756.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(33915..34325)
                     /gene="psiE"
                     /locus_tag="OO309_001580"
     CDS             complement(33915..34325)
                     /gene="psiE"
                     /locus_tag="OO309_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR5846757.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(34451..34594)
                     /locus_tag="OO309_001581"
     CDS             complement(34451..34594)
                     /locus_tag="OO309_001581"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846758.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            34595..34873
                     /locus_tag="OO309_001582"
     CDS             34595..34873
                     /locus_tag="OO309_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846759.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(34920..37016)
                     /locus_tag="OO309_001583"
     CDS             complement(34920..37016)
                     /locus_tag="OO309_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5846760.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNNLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(37016..37753)
                     /locus_tag="OO309_001584"
     CDS             complement(37016..37753)
                     /locus_tag="OO309_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5846761.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(37750..38388)
                     /locus_tag="OO309_001585"
     CDS             complement(37750..38388)
                     /locus_tag="OO309_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5846762.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(38502..38744)
                     /locus_tag="OO309_001586"
     CDS             complement(38502..38744)
                     /locus_tag="OO309_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846763.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(39207..40856)
                     /gene="pgi"
                     /locus_tag="OO309_001587"
     CDS             complement(39207..40856)
                     /gene="pgi"
                     /locus_tag="OO309_001587"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR5846764.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            41381..42730
                     /gene="lysC"
                     /locus_tag="OO309_001588"
     CDS             41381..42730
                     /gene="lysC"
                     /locus_tag="OO309_001588"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR5846765.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
BASE COUNT        10640 a        11011 c        10502 g        10628 t
ORIGIN      
        1 ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa caatataacc
       61 tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg gcggctcgtt
      121 agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc tgaactgatt
      181 gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac caccgatatt
      241 gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg tgccgccaac
      301 gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc atttacgggt
      361 acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc tcgccacttc
      421 tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca tctcgccggt
      481 cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg cgccaccatt
      541 tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa ccttgtttac
      601 gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag tgtaaacgcg
      661 cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc gggaaacaaa
      721 cctggccaga cattgttaca caacactccg gataatgcat tccaatactg tatattcatt
      781 caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat gccaccgggc
      841 aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg gcgcccgcac
      901 ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg tcgtgaccgg
      961 gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg aagggcagcg
     1021 ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga tggaaaagcc
     1081 ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga aatcgacgtc
     1141 tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt tgcgtttgtt
     1201 gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg cggcgcaaac
     1261 cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc tgatgctgct
     1321 cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga acctggcaag
     1381 ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg atccgccgaa
     1441 actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca aggtgcgtga
     1501 cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt ccggtggtac
     1561 agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct ccgccaactt
     1621 cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt tttcgtttaa
     1681 caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat atttcgatcc
     1741 tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc gtggctggga
     1801 tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact ataagttcga
     1861 cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt tgtacggttc
     1921 tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct ccatccgtcg
     1981 tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga cagaatccag
     2041 tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca gctgcgaagg
     2101 gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc tgcctgctat
     2161 ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac tcgctggtca
     2221 gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga aattcctcgt
     2281 aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt ccggcggtga
     2341 agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg ttatgtacgt
     2401 gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt tgggtacgct
     2461 tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg aagacgcaat
     2521 tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg gcggtgaagt
     2581 ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga ccgggcagta
     2641 catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga atccagaaaa
     2701 agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc tcacgctgcc
     2761 agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga cgctgattaa
     2821 cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg ccgaaccggc
     2881 accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata tcgaccaaag
     2941 cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt ttacgcctgt
     3001 gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc caggacgttt
     3061 cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg tgatcaaagt
     3121 ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag gtaaacgcta
     3181 taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag tgctggatat
     3241 gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc gtaagctgca
     3301 aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa ccacactttc
     3361 tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg gcaccgggca
     3421 gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata ttcagcaact
     3481 cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga ttgagcacaa
     3541 tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag gcggcagtgg
     3601 cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg aagcttcgca
     3661 tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg agcggccttt
     3721 tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt cactgcctgc
     3781 tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc gtcgagatac
     3841 acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct gtgctgctga
     3901 cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg attttctact
     3961 tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt ccagtcgtta
     4021 tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc cgaaatggtc
     4081 attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg gatgcgatgt
     4141 tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg taccggcaat
     4201 acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc tgctccctca
     4261 tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca ctccatgtcg
     4321 tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt atgttgcagc
     4381 aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt cagaatttca
     4441 tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt ttgataccac
     4501 atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc gaaaggataa
     4561 aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc tgtcagtatt
     4621 ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt ttgtaggtag
     4681 agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg gcggtaatat
     4741 cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat tgcgattttg
     4801 tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg gcaggaatat
     4861 gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta cgaccagtca
     4921 caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga gcgacctctt
     4981 ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca ggatttttca
     5041 gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc ggactggaaa
     5101 aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct gcgaggctat
     5161 tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc ctggcaacgt
     5221 tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa ctgtttagag
     5281 cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt ttccctaaat
     5341 agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct gaccataacg
     5401 ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc aggatgatta
     5461 tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt attactcgat
     5521 gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga gcgaaatttg
     5581 atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt acaaaaacaa
     5641 tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat ggtgattaat
     5701 tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag caacaaaacg
     5761 acatctttac ctgttgagct agatgcacta aataataaag atatctctta tgccaaagat
     5821 ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag acctggagaa
     5881 gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt agttaacaaa
     5941 atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc cgaggaggtt
     6001 aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca atataaggtt
     6061 aaacttacac aaagcggaaa aaagagttct ctttaacatt agaatttaac gatactataa
     6121 ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttgaggat atagaaatat
     6181 tctatcgtaa caaacccgat acttattatt aaatttaata atggttagat gtgcagaaat
     6241 tattactcca cacgaaaaaa tagggaaagg aattccagat aattgggata aacttaacaa
     6301 taaaagaact gacaacgttt tattatctct cacgtagaac aacggcatca aaaaaatcat
     6361 ctacaaagaa atagtgaaag cgacgacagt ttaatttcac tgcaggctgg gtaactccag
     6421 cctgctttcc tgcattacat caccgcagca aacgcctttg ccacacgttg tacatttgcc
     6481 gtatttaacc cggcgacaca catgcgaccg ctggcgatga gatagacacc aaattcttca
     6541 cgtagtcggt caacctgagc ggcacttaaa ccggtataac tgaacatgcc gcgctgatta
     6601 agcagataat cgaaattgcg ttctggcatc tctgtgctta ataccttcac caattcctga
     6661 cgcattgcca gaatgcgagt acgcatctct tctacttccg ccagccagct ggctttcaat
     6721 gcctcgtcat tcagcactgc agccaccacc tgcgcaccaa aattcggcgg gctggagtag
     6781 ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc cagcggcttc ggcatcttca
     6841 cacagaacag aaagtccgcc gacgcgctcg ccgtaaaggg agaaaatttt cgagaacgaa
     6901 ttgctcacca gagcgggtaa tccagcactg gcaatggcgc gaatagcgta ggcatcctct
     6961 tccataccgg cactaaatcc ttgataggca atatcgagga atgggataag ctcgcgggct
     7021 ttgagaattt caatcaccgc atcccactga tcattagtga gatcggcacc cgttgggttg
     7081 tggcaacatg gatgcagcaa cacaatactg cgggcaggta atgttttcag cgtcgccaac
     7141 aggtcattaa agcgcacgct gttagtcact tcgtcatacc aggggtaagt actcacttcg
     7201 aatccagccc cggcgaatat tgctacgtgg ttttcccaag taggatcgct gacccagacg
     7261 cctgattccg ggaagtagcg tttcaggaaa tccgcgccca ctttcaatgc ccctgagccg
     7321 ccaagggttt gaatggttgc tacgcgctgt tgttgcagta ccggatggtc cgcaccaaac
     7381 agcagcggcg caatggcatg gcgatagctg ttaagccctt ccatcggtaa ataaagcgaa
     7441 gcgccatggg gctgcgcatt caggcgcgct tccgcctccg ccacggcttt caattgtgga
     7501 ataattccgt cttcgttgta gtacagaccg atacttaaat tcactttgtc gctgcgaggg
     7561 tcttctttaa aacgctccat aagcgtaaga atcgggtcgc cagcgtaggc gtcaactttt
     7621 tgaaacacgc gatggttctc caggtttacg ggcaggtggt taaaacacaa taaaccggaa
     7681 gaaggcgaag atcgagtgga tgttcaggag cgaacggcaa ttagcaacag agtgagacta
     7741 atgataaacg tacatccgcc agaggcacga cctttataag agcatgaaag aacatcaact
     7801 tattgaattt ttaggatttt attggccgga taaggcattc acgccgcatc cggcacagac
     7861 aatcaaatat tacagaacga ttaatccacg tatttcatcg cgacccttga agtcaggcgc
     7921 gtaataagtt cgtaagcgct tactttcgtc atttcagcga tacgttctac gggcaaacct
     7981 tcgccccata aaatgaccgg atccccggct ttgtcctgcg cctgtggacc taagtctacg
     8041 cagatcatat ccatcgccac gcgcccgaca atcggtactt cgcgaccgtt caccagcact
     8101 ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat agcccatcgc gactacgcca
     8161 agacgggtat cacgttcgct tacccaggtt ccaccataac caacaggctc tccggcttta
     8221 tgctcacgca cggcaatcag gctggaggtt agtgacatca ctggctgaca gccaaaatcg
     8281 gcaccggtgg agcgatcttc cagcggcgag acgccataaa gaatgatgcc cgggcgcacc
     8341 cagtcaaaat gcgactgtgg ccacagcaga atgccacccg acgcggcaat ggaacgttga
     8401 ccaggtttgc cttcgcaaaa ggtattaaag atagcgagtt gtttctcggt tgcgccacat
     8461 tttggttcat ccgcgcgcgc aaaatggctg acgatattca ccggctgacg aacgtttttg
     8521 cactgggtca ggcgatgata aaacgcctca gcctgttccg gccttacgcc cagacggtgc
     8581 ataccggtat cgagtttcat ccagacggta accggctcgt ccaagctagc ctcttccagc
     8641 gcagccagct gttcttcgtt atgcacggcg gtatgaaaat gttgcgcaga aatcgtcggc
     8701 agatctctgg catcaaaaaa gccttcgagt aacagtacag gtttggtgat tccccccgca
     8761 cgcagtcgca gagcttcttc gagacgggct acgccaaagg cgtcagcatc ggggagcgtt
     8821 cgcgcggtct caagaagacc gtgaccataa gcgttcgctt tcaccaccgc aaccatttta
     8881 ctggcaggtg ccagttcacg aagacgttgc aggttgtgtc gcagagcgcg gcggttaatc
     8941 acaacagttg ccgcttgcat ttgtgttcct tgataattgt ttgctttaat tacctaattc
     9001 ataaaataat tattattcgt cgtcgtactg cggccccgca tagttgtcga agcgcgacca
     9061 ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg ttacgttgtt taccgataat
     9121 aatttccgcg atgcctttta aatcactgtt ttcgtgatac acctcatcac gatagataaa
     9181 catgatcaag tccgcatcct gctcgataga gccagattca cgcaggtcgg agttgaccgg
     9241 acgtttgtcg gcacgttgtt ccagagaacg gttcaactgg gacagcgcca ccaccggcac
     9301 gttcagttct ttcgccagtg ctttcagcga gcgggagatt tctgcaattt ccagcgtacg
     9361 gttatcggaa agcgccggta cgcgcatcag ttgcaggtag tcgatcatga taagcccgat
     9421 gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc acttccgttg gcgtcaggcc
     9481 ggaagagtca tcgatataga tattgcgttt ttcgagcaaa atacccatgg tgccggaaat
     9541 gcgcgcccag tcttcatcat cgagctgccc ggtacggatt ttagtctggt caacgcgcga
     9601 cagcgacgcc agagaacgca tcatgatctg ttctgatggc atctccagcg agaagataag
     9661 tactggttta tcctgcaaca ttgccgcgtt ttcgacgagg ttcatcgcaa atgttgtttt
     9721 acccatcgac ggacgcgcgg cgacgatgat caaatccgac ggctgcaagc cagcggtttt
     9781 tttgttgaga tcgtcataac cggtgtttac cccggtaacg ccatcgtgtg gctgctgaaa
     9841 caactgctca atacgcgcca cggttgcgtc gagcacatcg gcgatgttct tcggcccttc
     9901 gtctttgttt gcacgacttt cggcaatttt aaagacgcgg gattcagcaa ggtccagcag
     9961 atcttcgctg gtacgcccct gcggatcaaa accggcttca gcaatctcat tcgcaaccga
    10021 gatcatctca cggacaacgg cacgttcacg cacgatgtcc gcataagcac tgatgttcgc
    10081 cgcacttggc gtattttttg acagctctgc cagataagca aaaccaccga cgctatcgag
    10141 ttgcccctgg cgttccaacg attccgcaag ggtaatcaga tcgataggac taccgctttc
    10201 ctgcaaacgc gccatttcag taaagatatg acggtgtggg cgggtgtaaa aatcgtctgc
    10261 taccacacgc tcggctacat catcccagcg ttcgttatcc agcattaaac cgcccaacac
    10321 cgactgctcc gcttcgatcg agtgcggagg cactttcagc ccggcaactt gtggatcgcg
    10381 ttcgcggggt tcagcctgct gtttgttgaa gggtttattt cctgccatag tgaatggagt
    10441 taccgaaata aagaatgggt cgaaacttta ccatatgaag cagaccctga cgatacgttc
    10501 tggaggacac atggcaacac gaattgaatt tcacaagcac ggtggcccgg aagtacttca
    10561 agccgtagag ttcactcctg ccgatccggc ggagaatgaa atccaggtcg aaaataaagc
    10621 catcggcatc aattttatcg acacgtatat ccgcagcggc ctttacccgc cgccatcgct
    10681 acccagcgga ttaggtaccg aagcggcagg catcgtgagt aaagtcggca gtggtgtaaa
    10741 gcatattaag gctggcgatc atgtagtcta tgcgcagtcg gcgttaggcg cttacagctc
    10801 tgtgcataac attaatgcgg ataaagcggc gattctgcct gcggcaattt cttttgagca
    10861 agctgcggca tccttcctga aaggcttaac ggtttattat ctgctgcgca aaacctatga
    10921 aattaaaccc gatgaacagt tcctgttcca cgcagcggct ggcggcattg gcttaattgc
    10981 ctgccagtgg gcaaaagccc tgggcgcgaa actcatcggc accgtaggaa ccgcgcaaaa
    11041 agcgcagagc gcgctaaaag ccggcgcgtg gcaggttatt aactatcgtg aagagaatct
    11101 ggtcgagcga ttgaaagaga tcaccggcgg taagaaagtg cgcgtagtgt acgattccgt
    11161 gggcagagac acctgggaac ggtcgctgga ttgcctgcaa cgccgcggct taatggtcag
    11221 ttttggcaac tcatcaggtg cggttaccgg tgtgaactta ggcattctca atcaaaaagg
    11281 ctcgttgtat gtgacacgcc cttccctgca aggctatatc accacgcggg aggaattaac
    11341 cgaggccagt aatgaactgt tctctttgat tgccagcggt gtgattaagg tcgatgtcgc
    11401 cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg catgagattc tggaaagccg
    11461 ggcgacgcaa ggttccagcc tgctgattcc ataaaagaaa tagggcttcc acctgggaag
    11521 ccctttcttt ttatagttcg gctgtatgta gggtacagca cgatgaatct gttagaggcg
    11581 caatagtgac agatttgatt atcaattcct attttgttct aaggataaaa ccttaggttg
    11641 tgatcatccg cacaatccct tagtaacgcc agcggtcata acgctgatat ttcggcattt
    11701 ttggtgcttt aatcgcctta ataacccaca ccaccgcaat cgccagtagt aaccacggca
    11761 gcaacttaat catcaatgcc agcataccgc cgaggaacat aatggccgtc gccacaacca
    11821 gcgcggcgat aatgcccagc aacgaaacgc cggtgaccat cagcatgaca aaaaagccaa
    11881 tcacaaaaag tagttccagc atgatgctct cccaaatatg aaatctcttg ctggcattac
    11941 aagaatcatg ccaaaaataa tctattgatt taacagcaaa acgccccgcg acggtgcgca
    12001 gggcgtggtg aatttgacta ctttttggtg aaaagttaac gcttatccgc caccagtttg
    12061 agcgcgtgtt ccagcacgtt aatgtcagca cccgctttat gggcattttc acttaaataa
    12121 cgccgccact gccgcgcgcc aggaataccc tggaacaaac ccagcatatg ccggataata
    12181 tggccgagat acgtcccctg gctgagttca tgctcaatgt acggatacat ggcacgcact
    12241 actgccaccg gatcggcatc ggtatccgag gaaccaaaga tctcccggtc taccgccgcc
    12301 agaatacccg gattctgata cgcctcgcgc ccgaccatca cgccatccat atgttgcaaa
    12361 tgcgctttag cttcttccag cgacttgata ccaccgttaa tcgacattgt cagatgcggg
    12421 aagtcacgct tcagttgata cacacgcgga taatcgagcg gcgggatttc acggttttct
    12481 ttcgggctta accccgaaag ccaggcttta cgtgcgtgga tgatgaacat ctcacactcg
    12541 cctttgccgg aaacggtatt gatgaaatcg cagagaaatt catagctgtc ctggtcgtcg
    12601 atgccaatac gcgttttcac cgtcaccgga atcgacacca catcgcgcat cgctttcacg
    12661 cagtcggcaa ccagctgcgc attacccatc agacacgcac caaacatgcc gttctgcacc
    12721 cggtcagacg ggcagccgac attcaggttg atctcatcat atccgcgcgc ttctgccagc
    12781 tttgcacact gcgccagcgc cgccggatcg ctaccgccca gttgcaacgc taccggatgt
    12841 tcttcttcac tgtacgccag ataatcacct ttaccgtgaa ttatcgcccc tgtggtcacc
    12901 atttcggtat aaagcaacgt attgcgggaa agcagacgca agaaatagcg gcaatgtctg
    12961 tccgtccagt cgagcattgg tgcaacacta aaccgaccac tccagtgaac gtcagttttt
    13021 tcaggcattg cgctggtttg gttgattttt tgcatttcag aattaccgtg cattttcaaa
    13081 tgtagagatt attttatcga tatatcatgg ggttatgtta ttcagcttca ctgttcagga
    13141 ggctcaatag cggggtacta taccataaca acaggaagcg cctgtctcat tgcaaaagaa
    13201 aattgagatc ctctcaaggc atgaagctct cacgaagtga tggaaataat cttattagcc
    13261 gttaactttg ttaaggccaa tgataaacaa tccaggttcg acgataaata aaaaatcaca
    13321 cattaaactc tggtgatata tctccctgct aatagcattg atagagaaaa aagaacccaa
    13381 taaatattgg gtccttttat ataatgccct ccatactatc gaagaacttc acatattatt
    13441 tctccgattt aaccccgaac aagtcataaa ttaatttaga agtgtctgta agtattttaa
    13501 tctcttcctt tgaggttggg tcaaacgact tcgcgaagct aattaattgt ggtgcagcat
    13561 ctcgcatttt gcttaaaata tcaggatcga gcgttccttc attcaccaga tgtgacatct
    13621 tatccagata gccatcaaaa ttcatttctc ccccatctgc cagacgcttc atctctccca
    13681 ggtacttctt catatcacgt tgatataatt tttcaaactg agctttcagg tagctctcat
    13741 tattttcatt aatatacact gtttctgata aatctgcgta ggcactagaa tatgaaagtg
    13801 aaattaacag cgaagcaaat aattttaagc tgttgtcatc atgttcctgg caggcattaa
    13861 caaacgttaa aaaaccagaa cctattgctt gaaaatgtat attcgctaaa ggttcattat
    13921 ccttagattc ctcataaaat agctgaaccg tggagggaag gccattctta gtttctacat
    13981 caaaagtaca ttgttcaatg ggcattgcat ctttattatc cccaggatta catatactat
    14041 taatcgcgaa atgaaaaata gcccggtcaa caactgatgc aataaagtca cgatcgttta
    14101 aatcctcatc agtgcaatcg tcagtatact tattgaccca cattttgaga tcatttatgt
    14161 gctcaattaa aaacacattt ttttacgtgc atgccataaa ttatgcgtga ttcgttgaat
    14221 aaacaaatct atttttgcaa taattatatc ccgatgacgc agcgctgcat aatcaataga
    14281 tttaccatat atattattta atttaccttt gattgaacct ctattatcca atttatcttt
    14341 ggttgcatct ttattttcga attgatttga attgagtgtt ttattaaagt atccagttgc
    14401 accgggtatt atcttcaaca tgattcatct accttttttg tcgtaccgtg tcctccttat
    14461 aattcttttg ccatctggtc actatcaaaa atcgctcata atttctgaac tacaggtatg
    14521 cgtttaaacc cagttaaata aatagcgagt taacttgaag caatttattt aaaaatgaat
    14581 tattaattat tatcgtgttg ataatggtac gataaggcaa cacaacacct tacctacaaa
    14641 acagaaaatc aacattacgg caacaataag ggttctcggt gtttttcctg gaacatggta
    14701 aagtaaggac attcttaacc cccactttga ggtgcccgat ggaaaagacc acaacgcagg
    14761 agttattagc gcaggctgaa aaaatctgcg cgcagcgtaa tgtgcgcctg accccacagc
    14821 gcctggaagt gttgcgcctg atgagtctgc aggatggcgc tatcagcgct tatgatctgc
    14881 ttgatttact gcgcgaagct gaaccgcaag ccaagccgcc aacggtttat cgcgcgctgg
    14941 attttctgct tgagcaaggt tttgtgcata aggtggaatc caccaacagt tatgtgctct
    15001 gtcatctgtt cgaccagccc acgcatacgt cagccatgtt tatttgcgat cgctgcggcg
    15061 cggtgaaaga agagtgtgca gaaggcgtgg aagacattat gcatacgctg gcggcaaaaa
    15121 tggggtttgc cctgcggcat aatgtgattg aagcacatgg gctctgtgcg gcatgcgtgg
    15181 aagtggaagc gtgtcgtcat cctgaacagt gccagcatga tcactctgtg caggtgaaaa
    15241 agaaaccgcg ttaagagggc gtacatcctt gtacacgtcg ggcaggaggg attaattacc
    15301 agcgatattc attgcgggtt tcccaatcca cgacctcttt ttctgcctga tctttctgat
    15361 aaccataacg ttcctggatt ttaccgacca gttgatcacg tttaccttca atgatcgtca
    15421 tatcatcatc ggtcagtttg ccccattgct ctttcacttt acctttaaac tgtttccagt
    15481 taccgccggc ttcatcttta ttcataatca agacctcatc gttaggttgt gaatgagagt
    15541 acgttcactt ttcttctgaa cgtgagatta agtgtagtca gcaatttggc ttaggataat
    15601 ttattcagaa tttttaaccg tcacgttgcg gcaaaccagg taccgttgcg ccagtgacgc
    15661 cgccagatag ccgccagaga aagcccgcgc aacgccagaa agacggttaa tgccagccac
    15721 aaaccatgat tacccagcca cggcagcgta aggagcgtca gcgcaaaacc tgcggcggcc
    15781 accgccatac tgttacgcat ttcggcggca cgcgttgcgc ctataaacat gccgtccagc
    15841 agataacacc agacgccaac caacggcaaa atcacctgcc agataagata gcggtcagcc
    15901 agctgctgaa tctgggttaa cgacgtcagc aacgcaatga tgtgttcccc tgccagcaaa
    15961 taaaccaccg aaaatagtaa cgctacaatc cccgactggc ggcacgctgc ccgccataca
    16021 tccagcaact ggctaccgtc gcgcgcaccg tatgcctgac cggagtgcgc ttcaaccgcg
    16081 taggcaaaac catcaagcgc ataggcggta aaggtgagta gcgtcatcag aaccgcgtta
    16141 acagcgataa tgtcactccc cagtcgcgca ccaagtacgg tgatcgcgcc gaaacagagt
    16201 tgcaacaaca gcgaacgcag catgatatcg cggttaagcg ccagcaagcg acggaagttt
    16261 cctcgccagg cagttttcag catttcgccg gaaattccgc gtagtttgag gattttacgc
    16321 accattagca gaccaatcag caatgttgca tattccgcaa taaccgtcgc cagcgccgcg
    16381 ccctgcacgt tcatatgcag ccccatcacc agccagacat ccagcacaat gttgaggata
    16441 ttgccgacca ctaacaaaat tactggcgca cgggcatatt gcacgccgag taaccaaccg
    16501 agtaatacca gattcgccag cgacgccgga gcgcttaacc agcggatttc aagaaagcgc
    16561 cgcgcctgtt ccaggaccgc ttcactaccg ccaacaatat gcagcgccag atcgataatc
    16621 ggcgtacgca acagcgcaat taacgccccg gcccccaacg ccaacagcaa cggttgcacc
    16681 agcgcacggg ctaatgcctg aggatttttg gcaccataag cctgcgcagt cagcccggtg
    16741 gtgctcatgc gtaaaaacag caacagcata aagagaaagc tggtcgccgt tgcaccaacc
    16801 gccacgccgc ccaaataaac cggactatca agatgaccaa ttaccgccgt atcgaccagt
    16861 cccagcaacg gaacggtgat attggagaaa atcatgggta aggcgagatg ccagagtgct
    16921 ttatcagatg aagtgaggaa tgccatgcag acaagcctga tgaagagaga tgaaaaacaa
    16981 accgcgacgc caagcggcat cgcggtctca gagatatgtt acagccagtc gccgttgcga
    17041 ataaccccaa ccgccagccc ttcaatggtg aagctctgct gacgaaggtc aacgacaatt
    17101 ggtttaaact cgctattttc tggcaacagt tcgactttat tgccctgttt tttcaggcgc
    17161 ttaacggtaa cttcgtcatc aatacgtgcg acaacgacct gaccgttacg tacatcctga
    17221 gttttatgca ctgccagcaa gtcgccatcc ataatgccga tatctttcat cgacatcccg
    17281 ctgacgcgca gcaggaaatc agcattcggc ttgaacaagg aaggatcgac ctgataatga
    17341 ccttcaatat gctgttgcgc cagaagcggt tcaccggcag ccacacgacc taccagcggc
    17401 aacccttctt cctcttcctg caacagacga atcccgcgtg atgcgccgga aacaatttca
    17461 ataacgcctt tgcgtgccag cgccttcaga tgttcttcag ccgcgtttgg ggaacggaac
    17521 cccaaacgct gcgcgatttc cgcacgcgtc ggcggcatac ctgtctggct gatgtgatca
    17581 cggatgagat caaacacctc ttgttgcctg gccgttaacg ctttcattcc gccccctggg
    17641 tgtatataca gttatactgt gagtatatac agcaaaaggc gattttggaa ccataaactg
    17701 cacaataaac cagagattta tcgaaatctg gaagggttat ccaaaatgcg accataacag
    17761 gatgcaccag gtaatcacgg cgacgataat ggcaatcagc accgcggcgg atcccatatc
    17821 ttttgcgcgt ccggaaagct catggtattc agagccaatt cggtcaacca ctgcttcgat
    17881 ggcgctattg aggatttcca caatcatcac cagcatcacg gagctgataa gcagcacgcg
    17941 ggtaattgcg tccacatcca gccagcaggc gatgaccacc gccaacaata ccgctacgcc
    18001 ttcctgacgg aatgccgctt cgttgatcca tgcagcgcgt aaacctttcc aggaatagcc
    18061 agcagctttg ataattcggg tgaatccagt tgtattattg gccattgaaa gaaccttttt
    18121 acattatgag cgtcaatatc agtgtaccgt taaatccgat gattcgggtt tgcgtaacgc
    18181 cgcgcgaaac atgagcggat accacagaat ttcccatgac tttctgctat ccttgccgcg
    18241 catttgcatt attaaccaga ggctttacat cgtttatgtc cggctggcca cgaatttact
    18301 acaaattact gaatttacca ttaagcatcc tggtaaaaag caagtctatt ccggcagatc
    18361 ctgccccgga actggggctg gatacctctc gtccaattat gtacgtttta ccgtacaact
    18421 cgaaagcaga tttgctgacg ttgcgcgccc agtgtctggc acatgatttg cctgacccgt
    18481 tagagccgct ggaaatcgac ggcacgctac tgccgcgcta tgtgttcatt cacggcgggc
    18541 cgcgtgtatt cacctattac acgccgaaag aagagtctat taagcttttc cacgactatc
    18601 tcgatttgca ccgtagcaac ccaaatctgg atgtgcagat ggtgccagta tcggtgatgt
    18661 ttggtcgcgc gccggggcgt gaaaaaggcg aagtgaaccc gccgctgcgt atgcttaacg
    18721 gcgtacagaa atttttcgct gtactgtggc tcggtcgcga cagttttgtg cgtttctcgc
    18781 cgtcagtgtc gctgcgccgt atggcggatg aacacggcac ggataaaact atcgctcaga
    18841 aactggcgcg cgtggcgcgt atgcactttg cccgtcaacg tctggctgcc gtaggcccac
    18901 gtctgcctgc tcgtcaggat ctgtttaata agctgctcgc ctcccgcgcc attgccaaag
    18961 cggtagaaga tgaagcgcgc agcaaaaaaa tctcccatga aaaagcgcag cagaacgcga
    19021 ttgcgctgat ggaagagatt gcggcgaatt tctcttacga gatgatccgc ctgactgacc
    19081 gcattctggg cttcacctgg aaccgatttt atcagggcat caacgtccat aacgccgagc
    19141 gcgttcgcca gctggcccac gacggtcatg agctggtgta tgtgccttgc caccgcagtc
    19201 acatggacta cctgctactt tcttacgtgc tgtaccacca gggactggtg ccgccgcata
    19261 tcgccgccgg gatcaacctg aacttctggc cggccgggcc gattttccgc cgtctggggg
    19321 cgttctttat tcgccgtact tttaaaggca ataaacttta ttccaccgtt ttccgtgagt
    19381 atctcggcga actgttcagc cgtggttatt ccgtcgagta cttcgtggaa ggcggtcgtt
    19441 cccgcacggg gcgtttgctg gatccgaaaa ccggtacgct gtcgatgacc attcaggcga
    19501 tgctgcgtgg cggcacgcgt ccgattacgc tgattccgat ctatatcggt tatgagcacg
    19561 tcatggaagt gggtacttac gccaaagaac tgcgcggcgc gacgaaagag aaagagagcc
    19621 tgccgcagat gctgcgcggt ttaagcaagc tgcgtaatct cggtcagggt tacgtcaact
    19681 tcggtgaacc aatgccgttg atgacctacc ttaaccagca cgtaccagac tggcgtgaat
    19741 ctatcgatcc catcgaagcg gtgcgtccgg cctggttaac gccgacggtc aataatattg
    19801 ctgccgatct gatggtacgc attaacaacg caggcgcggc aaacgccatg aacctgtgct
    19861 gtactgcgct actggcatca cgtcagcgct cactcacccg cgagcagtta accgagcaac
    19921 tcaactgcta cctggatctg atgcgcaacg taccttactc cacggactct accgttcctt
    19981 cagccagcgc cagcgagctt atcgatcacg cgctgcaaat gaacaagttt gaagtcgaga
    20041 aagacactat cggcgacatc atcattctgc cacgcgagca agcggtgctg atgacctact
    20101 atcgcaacaa cattgcgcat atgttggtgc tgccttcgct gatggcggca attgtcaccc
    20161 agcatcgcca catctcccgc gacgtattga tggagcacgt caatgtgctt tacccaatgc
    20221 tgaaagcgga gctgttcctg cgctgggatc gcgacgagtt gccggacgtt attgatgcgc
    20281 tggcaaatga gatgcaacgt caggggctga ttaccctgca agatgatgag ttgcatatca
    20341 acccggcgca ttctcgcacc ctacagctgc tggccgcagg cgcgcgcgaa acgctgcaac
    20401 gttatgccat caccttctgg ttgttgagtg ccaacccgtc gatcaaccgc ggtacgctgg
    20461 agaaagagag ccgcaccgtt gcgcaacgtc tctccgtgct gcacggcatc aacgcgccgg
    20521 agttcttcga caaggcggtg ttcagttctc tggtgctgac gctgcgtgat gaagggtata
    20581 tcagcgatag cggcgatgcc gaaccagcag aaacgatgaa ggtttatcag ttgctggcgg
    20641 agttgattac atcagacgtg cgtttgacga ttgagagtgc gacgcagggc gaagggtaat
    20701 cagagagaat tgccggatgc ggcgaaaacg ccttatccgg cctaccatga cctgcaaatt
    20761 caataaattg cgattcaccg ggtaggcctg agaagcgcaa cgcatcaggc aacccagaag
    20821 aaagccggat gatcatccgg cttttttaca tcatcagaaa tgccagtaac tcattgccag
    20881 tcctaaaaac agtaccagac caacatagtt attattcata aatgctttaa agcaggcttc
    20941 acgctcgcgg ttggcaatca gtttttgttg ataaacaaac agcgcgccag ccaccacaat
    21001 tgaccagtaa tatccccagc ctaagccatt taactcaccg atgatcgcca tcagtgccag
    21061 tacgccaatc tgcaaaatac caataatcaa tttatcgtat tggccgaaca ggattgccgt
    21121 ggatttaatg ccaatcttca catcatcatc gcggtcaacc atcgcatact gcgtgtcgta
    21181 agccaccgcc cagagaatat tggcgaggaa cattaaccag caactcaatg gcaccgactc
    21241 actcacagcg gcaaaagcca ttggaatcga ccagccaaac gccgcgccca gcaccacttg
    21301 cgggagatgg gtataccgct tcataaacgg gtacacccac gccaatgcca gagcggcaat
    21361 cgacaacaga atggtcatcg tattcagcgt cagaaccagt aaaaacgaaa tcagtaccag
    21421 cactacaaac agcgcgcgcg cctctttctc tgttaccgcg ccgctgggaa gtggtcggtt
    21481 cgccgtgcgc ttaacatgac catcaaactt gcgatcagca taatcattca ccacgcaacc
    21541 agcagcgcgc atcagccaga cacccgcgac aaacaccgcc atgatccaga gctgaggaac
    21601 gcccggtgtc gccacccaca acgcccataa tgttggccag agcagcagta acgcgccaat
    21661 tggcttatcc gtacgcatta agcgatgaaa cgccagcagc ttattctgcg tcagactcca
    21721 ctccatattt ttttcctctt agtacaacgg tgacgccggt aaaaacagtt ctgttagcaa
    21781 cagcggttta ccgcttaatc gcaggcggga acgtcgcccc cacagtccgg catcacggcc
    21841 tatctcgata aaatcccggg ttagtgtcga tgatgtgaac agatagcgtc ctaacggcgt
    21901 tttacccaat ttttgtaacg ctagttccgg cccgcttaat gttgatacag gaacgacggt
    21961 acgaccggca agccacggtt caccatcggc acataacaaa atttcacgta accagtaacg
    22021 agactctttc ggcagcagcg gcagttcttc ggggatttca ttctgctcga caaacccctc
    22081 gcggatcatc gtcacactca ccgtttttcc ctgctgttca aaacgttttg tcatggaatc
    22141 ctccagcaac agccagtcga gcagttgcgg atccagggca gggatctctt taaaatagcg
    22201 cagcgcacgc agttgcgtta acgcggggtg tgacatgccg aactctccgt tacataacgt
    22261 aaaggcattg tatcgcagaa aagtgtttac gggagatgta aagcaaatta agtgatctaa
    22321 tgcgcaacaa aaacgcaaca atcgcatgaa gtggtaaaac gctgttgaac cttacgcttt
    22381 gccgatgccc gtgctggcga gggcaaccat aggggcatca ccgcccatga tgctaggaac
    22441 ggtgatgttt tcctttactt aaataccttt attctttata cgagtagctc gccaactcgc
    22501 agtaaaaatt gggaaaagcg catatttacc atgagatttg gagctgaaaa gtgtttcctg
    22561 agtaagatta ataggcaaca tcttatgtct ggatttatag atacacgtgg aattccaatt
    22621 ggtatctttt gtaggattca acatatcatg taagcgctgg gagcttacca ccataacagg
    22681 cccatcatcg acagaggaga acaggataaa tatattatcg tttcgtgtta atttattagt
    22741 gttaaaggtt cttaactttt cttcagtttc tataaatata ggtgaaacat catcactacg
    22801 aatccactct ttataaagat accaggcatt tgctttgatc atatcatccg ttgatcgtaa
    22861 ggcctgatcg ataagttgga taaaaaaatt gttattttta tcaaccagcc ctttattcat
    22921 attaaatctg ttaataaaat ggatcatctg ttgggtatcg aaattcacct tactgaatcg
    22981 atgttgacgc ataaaattat cgaacttgtc atcttcccca gaatgtatat aactacgtat
    23041 aattggatct tttatataag gacaatggct aaatgttttc ttcatattct caataaagtt
    23101 tttattaaca atattgagaa tttttaattg catcaccagt tttactgcca gaacggattt
    23161 caatatcatt gcattatctg gcaatgtatc aatcattttt ataatgttat tgatataacc
    23221 atcgagatct tcattcccaa atatatctga ataatcaata gttaattcca attgttctgt
    23281 aatgcaatct taaaaattcc ctgtcgacat tcgcacatga tcaaacataa cagctgtcaa
    23341 attcgattta ataaatgata tttctttggc agacagaaac tctgctctgg tgaagtgcga
    23401 agcgtaaaga taacaattat cgaagtttga atttctaatc tgacattcgc gaaaattact
    23461 atgaccaagt tgactctgcg agaagtcaac tgtatcaaga atattttggc taaaatcaac
    23521 acattcgcca ctcgttttga gaaaaatcac attgtgtaaa cgtcgacatt taaatatgac
    23581 attatcaact gttgccttag taaaattgca attctcaatt tctttacatt cgaaagttac
    23641 atgttgtaga tttgatgata aaaaagacac cttagaaata acgtcacctt taaaatgaca
    23701 atttgttaaa tcaaaatcag acaggtcgat atacccctgc gcatctttat ccatcagaaa
    23761 attacaagcg cattctattt tttcatttct gtagaatttt acacttatag tatcattact
    23821 aattgagact tcacgtaaca tatttattgc atgatttttt tcgttccgtt gttttatatt
    23881 taaaaatata tcactgatat aaataaagta atctctgttt aaacctgtca ccccattgaa
    23941 attacgtagt aaatcaagat ctactatatt atttccttgc accacatcat gtttcatcaa
    24001 agagttattg ccgccagaat ttgtgatatg acctatcata aaacctctgt aatacagaaa
    24061 taaaataacc tggaaaagta gcctgagcgt ccagacacta ccggcgaaag gaaaagaatc
    24121 tctgataatg cattgaaata atggacattc ttagtgaaga agaagtgaag tactataaaa
    24181 aatcgctcat atcccaggaa ggaagcataa aaaaaggtgc gccaggagac gcaccagttg
    24241 ttgcaaaatc agcactgtgg ggcgtaatta ccccttgcct tttacactgc tgataaaggt
    24301 ggaacgggca gatgtcgatc ccaggcgttc agcttcatca agcagtttta acgccttatc
    24361 aacatcacct ttcgcgacag cgtttttgat cgcggtatta aaataacttt ccgtgtcgtt
    24421 gagcatcggc tcgcttttct tcaccggcac cggagcaggt gcagccacag ctggtgccgc
    24481 cgtgttgcct accgtaaccg gagctggagc ggaagaacca aatagcggtc ctaccaacac
    24541 gctggagctg gagttcgttt tgactttcag tttcagtaag ccatcgctgg tatgacgagc
    24601 aaccggatcg ggaatatccg ggatcgagtt accgacgcct ttggcatagg ctttagccgg
    24661 gtcgagcagt tgggtcgtct gctggagatc tttttccgtg gtaaagacca gaacataaag
    24721 tttttgctgc cccaacgccg gtgtcaggcg cataacgcct tccagccgat ctgcactcat
    24781 cacgcctagt tcctggtagg tgaaataact gctggggaag aaggctgatg gggtcatgtt
    24841 ctgatcaaga atcagcacgt tcggcgcaaa aacgctggtt tgtttgttca cttcgctggt
    24901 cagcgtcagg gtcagttcgc caatgtttgc cgggacgctg tacgcagcaa ccggaccact
    24961 gatgccggga acgttcagtt gttggccgcc ggtcgccagt tgggtggtct gggttttaga
    25021 ttgatcgacc ggtgtccagg tgagttgttg cagcgcggca gatggaatgg ctggcgcgtc
    25081 gctggtgttt tgcggtacgt agttaacatc ggcaaggcta attccaggcg cgcttgccag
    25141 taaccctgct gataaacaga gggcgatgag acttttattc attttcattg ttttcacctc
    25201 aaaatctgga gctcagcggt agccaggcaa tagcgcgcta aacccgataa tcagaggggc
    25261 tttcgcccct ttcagataat gacaacctgt ttttatgccg gatgcggcgt aaacgcctta
    25321 tccggcccag gttttgctat taccaccaga tttccatctg ggcaccgaag gtccactcgt
    25381 cgctgtcgcc acgaccgaag ctgccgccgt tgaaatcagc aggaacggct ttgccgtagt
    25441 tcgggttaac cttgctatcg ccgttgtagt cgtaacccca tttctcatcc cacttggcgt
    25501 aggttgcgaa gacacgaata gccgggcgtg accagatgct gtcgccagcc tgccattgtt
    25561 gtgcgagggt aattttgtac tgattgttct tgtcgccggt gcgctgggat tcgacgttgt
    25621 cgtagccgat ttccatcacg gtgctcatga ttggcgtcca cttgtacatc gggcgaatac
    25681 caacggtcca ccacttggtg ccgttgtcgt tatcccagtt gatatcctgg tacataccca
    25741 cgtacatcat gtcccagttg tcgcccatgg agatcgcacc gtggtcgagg atacgcagca
    25801 tgtgaccgtt gttgttgata ttgtaggcaa atttttcgtt atcaaacgcg acgccagaac
    25861 cctgcgacag acctttaccc tgcgaggtca tcgagtcagt agcgtactga acaacaaact
    25921 tgttaaagcc cttcaggaca ctctgagtat gttcagcagt gaataaccag ccgtctttcg
    25981 atgcgccatc aaccagacga tagttatcac gcaggttggc acgaccgtag tcgacaccca
    26041 gttctaatgt gccgcccggg ttgatttcca tctgcgctaa acgcacatcg aaaacgtcgt
    26101 tcgcggtttc gttggtatag tcataaatat tgttgctggc aaaagaggaa gaaccaccag
    26161 cttcagagga gcgggttgct gccagagaga gtttaccgaa gccaacatcg atgttttcca
    26221 gaccggcacc aggaccagaa atatcccagt agtagaagtc gatcatatga acgtcatgac
    26281 gttggtagaa gcgcttacct gcccagatgg tggaacctgg cagccattcg atcaggtttt
    26341 ttccctgcac gtttgcttca cggaaggccg gatcggtagc ttcccagtca ttctgttgcg
    26401 cgacggaata ggccacgtta gtgtcgaaat agaagctctt atcgccctct ttccacactt
    26461 cctgacccaa ttttaattca gcataagttt cacattcgtt gccaagacgg tatttacttt
    26521 gagcaccggt agtctggaaa cactgttgtt caccgccgct acctgtccag ccaataccgg
    26581 aacgtgcata gccgtggaaa tcaacagcca ttgcctgagc agacattacg cccgctgcga
    26641 cggcaaccgc cagaggaagt ttgcgcagag taatcatcat tctatctcct gagtcattgc
    26701 ttttcttttt tcacatcacc tgtgacaggc tttgtgtgtt ttgtggggtg cttaaacgcc
    26761 cggctcctta tgcagtcgac gacatgcagt gccatcctca cggaacagat ggcaacgctc
    26821 tggcggcagg ccgatagcga atgtggcacc ttcttctacc aacaccacgt cgttctggcg
    26881 gtacaccagg ttttgacgaa tggaagggat ctggatatag atttgggttt cgttgccgag
    26941 ttgctcgacg acctgaactt caccctcaag gatgacgtca gcgatatcac tcggcagtag
    27001 atgttccggg cgaataccca gcgacatatt ggctccaacc tggacatcac ggctttcaac
    27061 aggcagccag acttgttgac gatttggcat cggcagctcc acctgcactt gatcgattgc
    27121 ggtggcggtc acttttaccg gcaggaagtt catctttggc gaaccgataa atccggcgac
    27181 aaaacggtct gccggatagt ggtacagttc cagcggtttc ccaacctgtg ccacgcgacc
    27241 ggcgtccagc accacgattt tgtcggccag cgtcatcgct tcgacctgat cgtgggtgac
    27301 gtaaatcatt gtgcggccca ggcgtttatg cagacgggag atttcgatac gcatttgcac
    27361 acgcagtgca gcatcgaggt tggagagcgg ttcatcgagc aaaaatacgc ttggctcggc
    27421 caccagcgta cggccaatcg ccacgcgctg acgctgacca ccggagagcg ctttcggttt
    27481 gcgatccagc aaatgcgcca gttgcagcac ttccgccacc tggttgacgc gttggttaat
    27541 cacctctttt tttgcgccag ccagtttcag gccaaatgac atgttttctg ctactgacag
    27601 gtggggatag agcgcgtaag actgaaacac cataccaacg ccgcgttctg ctggcggagt
    27661 gtcattcatc cgtttctcac cgatgaacag gtcgccgctg gtgatcgtct caagcccggc
    27721 aatcatgcgc agtaaagtcg atttaccgca gccagacggt ccgacaaaca ccacgaattc
    27781 accttcatgg atatcgagat tgatatcttt cgataccacg acctcgcccc aggctttcgt
    27841 tacattttgc agctgtacgc tcgccatgcc cttctccctt tgtaacaacc tgtcatcgac
    27901 agcaacattc atgatgggct gactatgcgt catcaggaga tggcttaaat cctccacccc
    27961 ctggcttttt tatgggggag gaggcgggag gatgagaacg cggcttctgt gaactaaacc
    28021 gaggtcatgt aaggaatttc gtgatgttgc ttgcaaaaat cgtggcgatt ttatgtgcgc
    28081 atccccacat taccgccaat tctgtaacag agatcacaca aagcaacggt ggggcgtagg
    28141 ggcaaggagg atggaaagag gttgccgtat aaagaaacta gagtccgttt aggtgttttc
    28201 acgagcactt caccaacaag gaccatagat tatgaaaata aaaacaggtg cacgcatcct
    28261 cgcattatcc gcattaacga cgatgatgtt ttccgcctcg gctctcgcca aaatcgaaga
    28321 aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg ctgaagtcgg
    28381 taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg ataaactgga
    28441 agagaaattc cctcaggttg cggcaactgg cgatggccct gacattatct tctgggcgca
    28501 cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc cggacaaagc
    28561 gttccaggac aagctgtatc cgtttacatg ggatgccgta cgttacaacg gcaagctgat
    28621 tgcttaccca atcgctgttg aagcgttatc gctgatttat aacaaagacc tgctgccgaa
    28681 cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag cgaaaggtaa
    28741 gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga ttgctgctga
    28801 cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg tgggcgtgga
    28861 taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa acaaacacat
    28921 gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg aaacagcgat
    28981 gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga attatggtgt
    29041 aacggtactg ccgaccttca agggtcaacc atctaaaccg ttcgttggcg tgctgagcac
    29101 cggtattaac gccgccagtc cgaacaaaga gctggcgaaa gagttcctcg aaaactatct
    29161 gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg ccgtagcgct
    29221 gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacca tggaaaacgc
    29281 ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt atgccgtacg
    29341 tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgaagccc tgaaagacgc
    29401 gcagactcgt atcaccaagt aatgctgtga aatgccggat gcggcgtgaa cgccttgtcc
    29461 ggcctacaaa actgaaacgc atgtaggcct gataagacgc atcagtgtcg catcaggcag
    29521 ttgttgtcgg ataaggcgtg aaagccttat ccgtcctgga atgaggaaga accccatgga
    29581 tgtcattaaa aagaaacatt ggtggcaaag cgacgcgctg aaatggtcag tgctaggtct
    29641 gctcggcctg ctggtgggtt accttgttgt tttaatgtac gcacaagggg aatacctgtt
    29701 cgccattacc acgctgatat tgagttcagc ggggctgtat attttcgcca atcgtaaagc
    29761 ctacgcctgg cgctatgttt acccgggaat ggctggaatg ggattattcg tcctcttccc
    29821 tctggtctgc accatcgcca ttgccttcac caactacagc agcactaacc agctgacttt
    29881 tgaacgtgcg caggaagtgt tgttagatcg ctcctggcaa gcaggcaaaa cctataactt
    29941 tggtctttac ccggcgggcg atgagtggca actggcgctc agcgacggcg aaaccggcaa
    30001 aaattacctc tccgacgcgt ttaaatttgg cggcgagcaa aaactgcaac tgaaagaaac
    30061 gaccgcccag cccgaaggcg aacgcgcgaa tctgcgcgtg attacccaga atcgtcaggc
    30121 gctgagtgac attaccgcca ttctgccgga tggcaacaaa gtgatgatga gctccctgcg
    30181 ccagttttct ggcacgcagc cgctctacac actcgacggt gacggcacgt tgacgaataa
    30241 tcagagtggc gtgaaatatc gtccgaataa ccaaattggc ttttaccagt ccattaccgc
    30301 cgacggcaac tggggggatg aaaagctaag ccccggttac accgtgacca ccggctggaa
    30361 aaactttacc cgcgtcttta ccgacgaagg cattcagaaa ccgttcctcg ccattttcgt
    30421 ctggaccgtg gtgttctcgc tgatcactgt ctttttaacg gtggcggtcg gcatggttct
    30481 ggcatgtctg gtgcagtggg aagcgttgcg cggcaaagcg gtctatcgcg tcctgctgat
    30541 tctgccctac gcggtgccat cgttcatttc aatcttgatt ttcaaaggat tgtttaacca
    30601 gagcttcggt gaaatcaaca tgatgttgag cgcgctgttt ggcgtgaagc ccgcctggtt
    30661 cagcgatccg accaccgctc gcacgatgct gattatcgtc aatacctggc tgggttatcc
    30721 gtacatgatg atcctctgca tgggcttgct gaaagcgatt ccggacgatt tgtatgaagc
    30781 ctcagcaatg gatggcgcag gtccgttcca gaacttcttt aagattacgc tgccgctgct
    30841 gattaaaccg ctgacgccgc tgatgatcgc cagcttcgcc tttaacttta acaacttcgt
    30901 gctgattcag ctgttaacca acggcggccc ggatcgtctt ggcacgacca cgccagccgg
    30961 ttataccgac ctgcttgtta actacaccta ccgtatcgct tttgaaggcg gcgggggtca
    31021 ggacttcggt ctggcggcag caattgccac gctgatcttc ctgctggtgg gtgcgctggc
    31081 gatagtgaac ctgaaagcca cgcgaatgaa gtttgattaa gggagataac aaaaatggca
    31141 atggtccaac cgaaatcgca aaaagcacgt ttgtttatta ctcacctgct actgctactt
    31201 tttatcgcgg cgattatgtt cccgctgctg atggtcgtcg ctatctcgct gcgtcaggga
    31261 aacttcgcga ccggtagcct gatcccggag caaatctcct gggatcactg gaaactggcg
    31321 ttaggtttta gcgttgaaca ggctgatggt cgcattacgc caccgccatt cccggtactg
    31381 ctgtggctgt ggaactcggt aaaggtcgct gggatttccg cgattggcat tgtggcgctc
    31441 tccaccacct gcgcctacgc tttcgcccgt atgcgctttc caggcaaagc gacgctgctg
    31501 aaaggaatgc tgattttcca gatgttcccg gcagtacttt cactggtcgc gttgtatgcg
    31561 ttgtttgatc gtctgggtga gtacattcca ttcattggcc tgaatactca cggcggcgta
    31621 attttcgcgt atctgggtgg gattgcgctg catgtctgga ccatcaaagg ctatttcgaa
    31681 accatcgaca gttcgctgga agaagcggct gcgctggatg gtgcgacacc gtggcaggcc
    31741 ttccgccttg tcctgttgcc gctgtcagta ccgattctgg cggtggtatt catcctgtcg
    31801 tttatcgctg ccattactga agttccggtc gcgtcgctgt tactgcgtga cgtaaacagc
    31861 tacaccctgg ccgtggggat gcagcaatac ctcaacccgc aaaactacct gtggggtgac
    31921 tttgccgccg ctgccgtgat gtctgcatta ccgatctcca tcgtcttctt gctggctcaa
    31981 cgctggctgg tcaacggcct gacggcaggt ggtgtgaaag gttaaagatg ttgttctgcc
    32041 aatgttatgc cgctgcaccc tcaacttacg ttatcccaac ttgtgactat tattcggcgc
    32101 tccacggagc gccttttttt ctttcgtctg caatctgaat cgttcgccgg ttaatattcc
    32161 catcatagaa cttattattt ttacgttatt tgttttccca cttacgataa ttctctttcg
    32221 tgctctgagt cacggcaata gtattgtttc atcaattttt gataattatc acaattaaga
    32281 tcacagaaaa gacattacgt aaacgcattg taaaaaatga taattgcctt aactgcctga
    32341 caattccaac atcaatgcac tgataaaaga tcagaatggt ctaaggcagg tctgaatgaa
    32401 tacccagtat aattccagtt atatattttc gattacctta gtcgctacat taggtggttt
    32461 attatttggc tacgacaccg ccgttatttc cggtactgtt gagtcactca ataccgtctt
    32521 tgttgctcca caaaacttaa gtgaatccgc tgccaactcc ctgttaggat tttgcgtagc
    32581 cagcgctctg attggttgca tcatcggcgg tgccctcggt ggttattgca gtaaccgctt
    32641 cggccgtcgt gattcactta agatttctgc tgtcctgttt tttatttctg gtgtaggttc
    32701 tgcctggcca gaacttggtt ttacctctat aaacccggac aacacagtac ctatttatct
    32761 ggcaggttat gtcccggaat ttgttattta tcgtattatt ggcggtattg gcgttggttt
    32821 agcctcaatg ctttcaccaa tgtatattgc ggaactggct ccagctcata ttcgcggaaa
    32881 actggtttca tttaaccagt ttgcgattat tttcgggcaa cttttagttt actgcgtaaa
    32941 ctattttatt gcccgttccg gtgatgccag ctggctgaat actgacggca ggcgttatat
    33001 gtttgcctcg gaatgtatcc ctgcactgct gttcttaatg ctgctgtata ccgtgccaga
    33061 aagtcctcgc tggctgatgt cacgcggcaa gcaagaacag acggaaagta tcctgcgcaa
    33121 aattatgggc aacacgcttg caactcaggc agtacaggaa attaaacact ccctggatca
    33181 tggccgcaaa accggtggtc gtctgctgat gtttggcgtg ggcgtgattg taatcggcgt
    33241 tatgctctcc atcttccagc aatttgtcgg catcaatgtg gtgctgtact acgcgccgga
    33301 agtgttcaaa acgctggggg ccagcacgga tatcgcgctg ttgcagacca ttattgtcgg
    33361 agttatcaac ctcaccttta ccgtactggc aattatgact gtggataaat ttggtcgtaa
    33421 gccactgcaa attatcggcg cactcggaat ggcaatcggt atgtttagcc tcggtaccgc
    33481 gttttacact caggcatcgg gtattgtggc gctactgtcg atgctgttct atgttgccgc
    33541 ctttgccatg tcctggggtc cggtatgctg ggtactgctg tcggaaatct tcccgaatgc
    33601 tattcgtggt aaagcgctgg caatcgcggt ggcggcccag tggctggcga actacttcgt
    33661 ctcctggacc ttcccgatga tggacaaaaa ctcctggctg gtggcccatt tccacaacgg
    33721 tttctcctac tggatttacg gttgtatggg cgttctggca gcactgttta tgtggaaatt
    33781 tgtcccggaa accaaaggta aaacccttga ggagctggaa gcgctctggg aaccggaaac
    33841 gaagaaaaca caacaaactg ctacgctgta atcttcctgt ccagcacgcc gcgccatttc
    33901 ggcgtgctgt ctttttactc ccgcttcagc cgtttcgaat tacacagcca cagggtgatc
    33961 accagtaaca ggatcgcagc cgagtagatc aacacatcca gtggcgattt atgatcgacg
    34021 atgatcaagc gcacaatggc ggtgatccca atatagacaa agtaacgtag cgggaagtga
    34081 aaaccggact gaaagtactt cacaatcagc gcgataaatt cgaaatagag aaagtaaacc
    34141 accagtcctt ctaccagctc atatttgctg gtttgttctg gcgcgaacag cacatcagcc
    34201 agatgcaccg tttctttgcc gaggaagaca accaaaatca ggccgaggca cagcaggcca
    34261 agattgagta cggtctgcaa aatggtggag ataaactcca cgcgcggacg agagagtgac
    34321 gtcatagcag tacctccttc acagtggcga gcttcctgta taacgcaaaa atgtgacgga
    34381 gatctatatt ttttgtgtat gttttgttca acctggattt ggagcgcgcc tccaccaaag
    34441 ccggatatac ctacccgtag gccggataag ccgcgcctgg cggcgcatcc ggcaagacaa
    34501 accgcaccaa agccggtaat tcaaaaatca cccattcagg tgattcccag ccaccagcaa
    34561 tgcgcgaaaa atcgacaatg ttcctctgga acaaatgaaa cgtaacctga taaaggtggt
    34621 aaaaatgaag ccttattttg ctgctttgat gttatcagtc tctgttcttc ctgcttatgc
    34681 tggtccgctc ggtactgctg ataaagccga cctaccgcaa agcaatgtct ccagcccaat
    34741 gatggcccag tcgctcaggc aaccagacct gcaaccgata tcgacggaca ggaagacaga
    34801 atgttttcgc ctttacacac cagatcgtaa accgggggtg aactgcgttc ctgatggctc
    34861 aacaggtcat taagaatgag ggtgccagtg agtgcgaaaa ctggcacctt tcccatgact
    34921 tagcggaaat ttacgctacg gtcgctggtc atgtcataca actggaactt acgcccaagt
    34981 tgctgaccac cgtcacgcgt cagcggcgtc cagccaatcg ccgcacggct gcgtgtcggg
    35041 ccagacgaga agagatccaa cggtaccgag acatacacgc ctttggtgaa gtccccttcg
    35101 ccgtactcct ctttcgaaac gttggtgatc gtggcatagc cacccaccac cacgccgctg
    35161 tcaaagcgtt tagcgatctc cagcgtgccg cctttatccc ctgccagata ctgcccgacg
    35221 ctggctttca ccaacacgtc ctgggcgaaa gatggcgtcc agtaggcggt cagatgtccg
    35281 gttttcacgc tgtagtcggt gaatttcatc atatcttttg cactacgcca gtcgcgctgt
    35341 ttaacgtagt tggcatccag accaaacgcc cagttactgt ccagcggacg atacaacact
    35401 tcggcccccg caccgccaaa catggtttcg agataacctc catagacctg accgtagaag
    35461 ccgttgccca gatgctggaa gtagttggct tgcaggttat tcacatagac atcgttctgc
    35521 acatactcgc gcacatgggt acgaacgcgc ggtaagtgcg agtcctgcgg tggattggtg
    35581 tagttaaact tgtcgtagtt gttggcaaga tttgcaaaca ggctgccggt ggtcagtaga
    35641 tggtccgtca gccacaaatc tgccgttccc atcacgccca gctgatacat gtaaaagttt
    35701 tccgggccac cgaccgactg gttcagcacc ggatcgatat ggaaatcaaa gcgtgatttg
    35761 tcgatatacc agccctgctc ggtggacttc ggaaccactg gctcgacgcg tttgtgcgcc
    35821 agcgtcgttt cgtggcccaa cggctctccg gcgagattat ttttcaggct ggcgacatcg
    35881 gtttccgtcg tcacttgcgg catgttaagg cgattttccg taatgcggat cgttttgatc
    35941 ccatccggca gatcgttcat cacgatccga ttagcacgga tgatcccttc gcgcgaatca
    36001 cgatatttca cctgctcgcc ggtaacatac agcgtatcgc ctttcgcctg gatctgcgga
    36061 tcggcaagtc cggcgttgta tttcagcaac gttaactgat tcgccaccac cgaatgctgc
    36121 aaaatggcat cctgcggctg cggttgatat tgcgggcggg cgttatcgat gtaggacggg
    36181 cgcagatcgt taaagttggt gcgcagcgtg acgccaaaca taaaggtgtt gccacgttca
    36241 tagctaaggt taacgtcggc ccaatcggta acgcgataaa tcgccccgac gttaaactta
    36301 cttttttgct ccagcttccc ggcaaaatcc tgctgataat tattaccttc atactccagt
    36361 ttcagacgca gcggttgcca gggcgtctgg tattccacgc cgccaaacag tgaggcagga
    36421 ccgtggaaca tctggctacc gtcgatagat cccgcctgtt tgtagctgtt atcgcgatag
    36481 caatatttat cactggctga acagagcgga ttttttacat taccgctggt gcccagatat
    36541 ccccagccca ggccgagcgt aaaatcgaac ggcccccagg ctttgctggc aacaagatat
    36601 tccgcatcaa acagccccgt accgccgata tcccgcgcac caaccgccac ttgcggcagc
    36661 cagtaactct cttcccacaa acgcagtttg agatcgaagg ctttatcttt atacgtttga
    36721 tcgccagaga acgcttcgac gctgctgtac tgccgggtgc gcacgtcggt gtagcgcagc
    36781 gttgtttcca gccacgggaa gagttgcact gaagctgagt aataacggta ctgatcgtta
    36841 tcgcgatagt tcagactcaa ctccccttcc cgtgccatac gcgcggtggg agtttgtaat
    36901 aatcctacgc caccgaaatc cgactgcgac ggaccaatgg gcgcaggata tgtttcgccg
    36961 tagcaagctg tgctaatgcc cagcgccagt aagctaagca gatgtctttt tttcattatt
    37021 ggggtatccg ctgcgtcaga gtctgaagaa tgtcggcgtt aagggcatca ggcgtctcac
    37081 tccagacgga atctgcgagg ccaacataaa taatgctgcc gggcatcggc tctacgtgac
    37141 gcttgttcca gtaagccacc ggcgcttttt gcgtgcgtcc gtccgggtaa acgacccacg
    37201 cgtagctgcg atccgcaccg ctgagcaggc tctggtcaga gagatagctc gccacgtcgc
    37261 gacctggagt gaatggctga ttgccaggat ggctgataag cccgaacaac gtgaccgtgg
    37321 acggcggtgg tccgacccac agcgtgtagt tgccctgcaa cggcgggtta ccgcgttcgg
    37381 caacgcggac gatatcggga tcaagattga ttttttgcct gcctgtcact ttcaatgcct
    37441 ggatttgctg gcgtaaggcg ttaatggcgg cggcatcgtc ggtgctggaa tctgcgccct
    37501 gttctgccag ccgtgtcagc aacgcctgct gctgacgcaa tgctgccgcc gttgccagct
    37561 cttcgctaat caccgcacca ggccaccagc tgtttgccag tcgaggttgc cctaccagat
    37621 cgattaaatg ctctgcgccc gttagcgttt tggcctcact gctgccattg ctgaacacct
    37681 tgacggttcc tgccgcgaag accgatgacg ctcccacact caaaagcaac gcgacaatag
    37741 tttgtttaat cattgttttg ccgccttgat gagagtggtt ttcaccggga aataatccgc
    37801 gccgagatac tgttccgact ggcgaatttg cccttcgctg tcgatccagt agcggttatg
    37861 ccagcgcgcc tggtcggtgg agacttcttc gtccagcacg cgaaccaggg tttcatcgct
    37921 gccgaccttg acggtatcgg tgccatccca tttgaagact gagcgtgcgg tggcgtagcg
    37981 tacctgctgg tactcggtcc agcccatcgt gcgcgtccat gttgcaccat caacaatttg
    38041 tgcgggttta atcagcgggt cggcggcaag gttattcact tcaatcaggt tgtcgccgcc
    38101 aagcaaagtc ttcaccagac gaccatgttg tgtgacaaga gtggcttgat cctgagtgac
    38161 ccatttttgt tgtccgtctt cagcgaaggc cagcaccaca aacaattgcg gcccgccatt
    38221 aagctgcatg tactggctgg cgtagggcat attttgaata tcatcgtccg tcagctgtac
    38281 gcctggcgtg ccgaacagac tgtcccacag tgaattgccc agctctttag tcgtggctga
    38341 acaggcctgt aatagcaggc agataagaat gagtgcaggt cgcttcacga ctcttctcca
    38401 gaggggcaga ataaccacac cggagtgtgg ttatggtaaa tcccttcata cttcaagcca
    38461 cagatgcaat gactcaaatt attttgggga tacatacttt attactgggt actggtggtc
    38521 gtggttgtgg tggtcccggt attagaacca tcaccgccac cggttgccgc gagcgcgaca
    38581 cccaccgccg agcttacggt gctgacgctg gtcgcggagg aactccccgc cgaaaccgac
    38641 gttgctgccg actctgccgc ttcgcccacc tgtacaggtg cagcccacgc agaagtcgcc
    38701 gcaagcgcag atatggcaaa aatgccatac agaacttttt tcataacaat ttcccttcat
    38761 tgaatgaatg gagatttacc caaaaacatt tcgggcggga tcgagtatac acaactaaag
    38821 catgcgaatt acggcgcggg gaattagcga tggagtttta ggataattgg aatgaatatc
    38881 aaatataagt ttcaacttat atttaaataa caataaaaat cataagctta aaataaaaaa
    38941 caaaagtaat taatctgcgt attctccgaa ataaacaacg attaaaaatc tattttagga
    39001 ttaacctcac ggtatgattt ccgttaaatc acagacaagg ggatttatct gataaaacaa
    39061 tgccgactta ttgtcggcat tgtttttaag gttatttaac tcctcatact atgcattaat
    39121 gtcgatattg gcatcaggct ccggttgccg gatgcggcgt aaacgcctta tccggcctac
    39181 atatcggcat caggcacgca tgatgattaa ccgcgccacg ctttatagcg gttaatcaga
    39241 ccattggtcg agctatcgtg gctgctgatt tctttatcat ctttcagctc tggcagaata
    39301 cggtttgcca gttgtttacc cagctccacg ccccactggt cgaaggtgaa gatgttcagg
    39361 atcacgccct gagtaaagat tttgtgctca tacagcgcaa tcaacgcacc caggctgaac
    39421 ggagtgattt cgcgcagcag gatggagttg gtcgggcggt taccttcgaa cactttgaac
    39481 ggcaccacgt agtcaagcgt tgccggatct ttaccctgat cgcgatattc ctgctcaacc
    39541 acttcgcggg atttaccaaa cgccagcgct tcggtctggg cgaagaagtt agacagcagt
    39601 ttctggtggt gatcagagag cgggttatgg gtgatagccg gggcgatgaa atcgcacggt
    39661 accattttgg ttccctggtg gatcagctgg tagaacgcgt gctgaccgtt agtgcctggt
    39721 tcaccccaga taatcgggcc agtctggtaa tccacaacgt taccgttacg gtcaacatac
    39781 ttaccgttgg actccatatt gccctgctgg aagtacgccg cgaaacggtg catatactgg
    39841 tcatacggca gaatcgcttc agtttccgca ccaaagaaat tgttgtacca gatgccaatc
    39901 agcgccagca ggacaggcag gtttttctcg gcaggcgtgg tggagaaatg cttgtccatt
    39961 gcgtgtgcgc cagaaagcag ttcaacgaag ttatcaaagc cgatggagag aacaatcgac
    40021 aggccaatcg ctgaccacaa agagtaacgg ccgccgaccc agtcccagaa ctcgaacatg
    40081 ttggcagtat caataccaaa ctcgccaacg gctttggcat tggttgagag cgccgcaaaa
    40141 tgtttcgcaa cgtgtttttc atcacctgcc gctttcagga accagtcacg cgcgctatgg
    40201 gcgttggtca tggtttcctg agtggtgaag gttttagatg ctaccaggaa cagcgtggtt
    40261 tccgggttta cttttttcag cacttccgag atgtgagtcc catcgacgtt agaaacaaag
    40321 tgcatgttca ggtggttttt gtacggacgc agtgcttcgg tcaccatgta cgggccgagg
    40381 tcagaaccgc cgatcccgat gttcactacg tcagtgattg ctttgccggt ataacccttc
    40441 cactcaccgg agataatcgc ttccgagaag gttttcatct tctccagcac cgcgttgact
    40501 tctggcataa catctttgcc atcaaccaga atcggggtat tgctacgatt acgcagcgct
    40561 acgtgcagca cggcgcggtt ttcagtgcgg ttgatcttct cgccagagaa catcgactta
    40621 atcgcgcccg ccagatcgca ctctttcgcc agatcctgta atttcgccag cgtctcttca
    40681 gtgatgcggt ttttggagta atccaccagc atctgatcgt cgaaggttgc ggagaactta
    40741 gaaaaacgat cgccgtcttt agcaaaaaga tcggcgatcg taacgtcttt catttcatcg
    40801 aagtgtttct gtagtgcctg ccaggcagcg gtctgcgttg gattgatgtt tttcattagc
    40861 aatactcttc tgattttgag aattgtgact ttggaagatt gtagcgccag tcacagaaaa
    40921 atgtgatggt tttagtgccg ttagtgtaat gttgagtgta aacccttagc gcagtgaagc
    40981 atttattagc tgaactactg accgccagga gtggatgaaa aatccgcatg accccgtcgt
    41041 tgacaaccgc cccgctcacc ctttatttat aaatgtacta cctgcgctag cgcaggccag
    41101 aagaggcgcg ttgcccaagt aacggtgttg gaggagccag tcctgtgata acacctgagg
    41161 gggtgcatcg ccgaggtgat tgaacggctg gccacgttca tcatcggcta caggggctga
    41221 atcccctggg ttgtcaccag aagcgttcgc agtcgggcgt ttcgcaagtg gtggagcact
    41281 tctgggtgaa aatagtagcg aagtatcgct ctgcgcccac ccgtcttccg ctcttccctt
    41341 gtgccaaggc tgaaaatgga tcccctgaca cgaggtagtt atgtctgaaa ttgttgtctc
    41401 caaatttggc ggtaccagcg tagctgattt tgacgccatg aaccgcagcg ctgatattgt
    41461 gctttctgat gccaacgtgc gtttagtagt cctctcggct tctgctggta tcactaatct
    41521 gctggtcgct ttagcggaag ggctggaacc tggcgagcga ttcgaaaaac tcgacgctat
    41581 tcgcaacatc cagtttgcca ttctggaacg tctgcgttac ccgaacgtta tccgtgaaga
    41641 gattgaacgt ctgctggaga acattactgt tctggcagaa gcggcggcgc tggcaacgtc
    41701 tccggcgctg acagatgagc tggtcagcca cggcgagctg atgtcgaccc tgctgtttgt
    41761 cgaaatcctg cgcgaacgcg atgttcaggc acagtggttt gatgtacgta aagtgatgcg
    41821 taccaacgac cgatttggtc gtgcagagcc agatgtagcc gcgctggcgg aactggccgc
    41881 gctgcagctg ctcccacgcc tcaatgaagg cttagtgatc acccagggat ttatcggtag
    41941 cgaaaataaa ggtcgtacaa cgacgcttgg ccgtggaggc agcgattata cggcagcctt
    42001 gctggcggag gctttacacg catctcgtgt tgatatctgg accgacgtcc cgggcatcta
    42061 caccaccgat ccacgcgtag tttccgcagc aaaacgcatt gatgaaatcg cgtttgccga
    42121 agcggcagag atggcaactt ttggtgcaaa agtactgcat ccggcaacgt tgctacccgc
    42181 agtacgcagc gatatcccgg tctttgtcgg ctccagcaaa gacccacgcg caggtggtac
    42241 gctggtgtgc aataaaactg aaaatccgcc gctgttccgc gcgctggcgc ttcgtcgcaa
    42301 tcagactctg ctcactttgc acagcctgaa tatgctgcat tctcgcggct tcctcgcgga
    42361 agttttcggc atcctcgcgc ggcataatat ttcggtagac ttaatcacca cgtcagaagt
    42421 gagcgtggca ttaacccttg ataccaccgg ttcaacctcc actggcgata cgttgctgac
    42481 gcaatctctg ctgatggagc tttccgcact gtgccgggtg gaggtggaag aaggtctggc
    42541 gctggtcgcg ttgattggca atgacctgtc aaaagcctgc ggcgttggca aagaggtatt
    42601 cggcgtactg gaaccgttca acattcgcat gatttgttac ggcgcatcca gccataacct
    42661 gtgcttcctg gtgcccggcg aagatgccga gcaggtggtg caaaaactgc atagtaattt
    42721 gtttgagtaa atactgtatg gcctggaagc tatatttcgg gccgtattga ttttcttgtc
    42781 a
//
LOCUS       DAJTQQ010000027        40541 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000027 DAJTQQ010000000
VERSION     DAJTQQ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.027"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..956)
                     /locus_tag="OO309_001589"
     CDS             complement(<1..956)
                     /locus_tag="OO309_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR5846766.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="OO309_001590"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="OO309_001590"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCR5846767.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="OO309_001591"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="OO309_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="OO309_001592"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="OO309_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR5846768.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="OO309_001593"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="OO309_001593"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR5846769.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="OO309_001594"
     CDS             complement(5018..6346)
                     /locus_tag="OO309_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5846770.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..8696)
                     /locus_tag="OO309_001595"
                     /pseudo
     CDS             complement(6354..8696)
                     /locus_tag="OO309_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8874..9785)
                     /gene="gsiD"
                     /locus_tag="OO309_001596"
     CDS             complement(8874..9785)
                     /gene="gsiD"
                     /locus_tag="OO309_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR5846771.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9788..10708)
                     /gene="gsiC"
                     /locus_tag="OO309_001597"
     CDS             complement(9788..10708)
                     /gene="gsiC"
                     /locus_tag="OO309_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR5846772.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10726..12264)
                     /gene="gsiB"
                     /locus_tag="OO309_001598"
     CDS             complement(10726..12264)
                     /gene="gsiB"
                     /locus_tag="OO309_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR5846773.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12284..14155)
                     /gene="gsiA"
                     /locus_tag="OO309_001599"
     CDS             complement(12284..14155)
                     /gene="gsiA"
                     /locus_tag="OO309_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR5846774.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14142..15107)
                     /gene="iaaA"
                     /locus_tag="OO309_001600"
     CDS             complement(14142..15107)
                     /gene="iaaA"
                     /locus_tag="OO309_001600"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5846775.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15311..16546
                     /gene="moeA"
                     /locus_tag="OO309_001601"
     CDS             15311..16546
                     /gene="moeA"
                     /locus_tag="OO309_001601"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR5846776.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            16546..17295
                     /gene="moeB"
                     /locus_tag="OO309_001602"
     CDS             16546..17295
                     /gene="moeB"
                     /locus_tag="OO309_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR5846777.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17474..18136)
                     /gene="fsa"
                     /locus_tag="OO309_001603"
     CDS             complement(17474..18136)
                     /gene="fsa"
                     /locus_tag="OO309_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR5846778.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18267..19166
                     /locus_tag="OO309_001604"
     CDS             18267..19166
                     /locus_tag="OO309_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR5846779.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19172..21604
                     /locus_tag="OO309_001605"
     CDS             19172..21604
                     /locus_tag="OO309_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR5846780.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21750..22565
                     /gene="ybiV"
                     /locus_tag="OO309_001606"
     CDS             21750..22565
                     /gene="ybiV"
                     /locus_tag="OO309_001606"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR5846781.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22717..23982
                     /locus_tag="OO309_001607"
     CDS             22717..23982
                     /locus_tag="OO309_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR5846782.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24223..25815)
                     /locus_tag="OO309_001608"
     CDS             complement(24223..25815)
                     /locus_tag="OO309_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR5846783.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26034..26954
                     /gene="ldtB"
                     /locus_tag="OO309_001609"
     CDS             26034..26954
                     /gene="ldtB"
                     /locus_tag="OO309_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5846784.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27012..28130)
                     /locus_tag="OO309_001610"
     CDS             complement(27012..28130)
                     /locus_tag="OO309_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR5846785.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28127..28594)
                     /gene="mntR"
                     /locus_tag="OO309_001611"
     CDS             complement(28127..28594)
                     /gene="mntR"
                     /locus_tag="OO309_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR5846786.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28780..28908
                     /gene="mntS"
                     /locus_tag="OO309_001612"
     CDS             28780..28908
                     /gene="mntS"
                     /locus_tag="OO309_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR5846787.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29180..30763
                     /gene="opgE"
                     /locus_tag="OO309_001613"
     CDS             29180..30763
                     /gene="opgE"
                     /locus_tag="OO309_001613"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR5846788.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30811..31326)
                     /gene="ompX"
                     /locus_tag="OO309_001614"
     CDS             complement(30811..31326)
                     /gene="ompX"
                     /locus_tag="OO309_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR5846789.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31379..31444)
                     /locus_tag="OO309_001615"
     CDS             complement(31379..31444)
                     /locus_tag="OO309_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846790.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31679..32566
                     /gene="rhtA"
                     /locus_tag="OO309_001616"
     CDS             31679..32566
                     /gene="rhtA"
                     /locus_tag="OO309_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR5846791.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32865..33368
                     /gene="dps"
                     /locus_tag="OO309_001617"
                     /gene_synonym="pexB"
     CDS             32865..33368
                     /gene="dps"
                     /locus_tag="OO309_001617"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR5846792.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33772..34518
                     /gene="glnH"
                     /locus_tag="OO309_001618"
     CDS             33772..34518
                     /gene="glnH"
                     /locus_tag="OO309_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR5846793.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            34657..35316
                     /gene="glnP"
                     /locus_tag="OO309_001619"
     CDS             34657..35316
                     /gene="glnP"
                     /locus_tag="OO309_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR5846794.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35313..36035
                     /gene="glnQ"
                     /locus_tag="OO309_001620"
     CDS             35313..36035
                     /gene="glnQ"
                     /locus_tag="OO309_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR5846795.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36296..38520
                     /gene="ybiO"
                     /locus_tag="OO309_001621"
                     /pseudo
     CDS             36296..38520
                     /gene="ybiO"
                     /locus_tag="OO309_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38517..39443)
                     /gene="rlmF"
                     /locus_tag="OO309_001622"
     CDS             complement(38517..39443)
                     /gene="rlmF"
                     /locus_tag="OO309_001622"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR5846796.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39719..39979
                     /locus_tag="OO309_001623"
     CDS             39719..39979
                     /locus_tag="OO309_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5846797.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            40244..>40541
                     /locus_tag="OO309_001624"
     CDS             40244..>40541
                     /locus_tag="OO309_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846798.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVP"
BASE COUNT        10096 a        10717 c         9864 g         9864 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattgctgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgct
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctgatgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaagca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttaacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctgatgtct ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg
     7981 ctattataaa taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct
     8041 tgtttcaaaa atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac
     8101 cgccagttta aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg
     8161 atacgtagcg taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga
     8221 ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa
     8281 tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga
     8341 taaacaacga ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt
     8401 accagtccca ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga
     8461 atctcctctg tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca
     8521 atatctttaa agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt
     8581 tttaccgcgc gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac
     8641 agtgctgcca ataaaaataa aattatcagc cttatctttt cgtataaact cagcatgtta
     8701 atacatttaa caaaataact atctgaacgc tgtgccgctg caaaaatagt cccttgctgc
     8761 ggctttttaa ccaaattacc cgattctaca aaatcagcag aaagttacaa cacttgctta
     8821 gggcaataat ttcctgtgag gtgattaccc tttcaagcaa tattcaaacg taactatcct
     8881 ttaattttcg gatccagcgc atcgcgtaaa ccatcgccca acaaattgaa cgccagtacg
     8941 gtcagaaaaa tagccagggc cggaaaaaca gcgacatgcg gcgcgataac catatccgct
     9001 cgtgcctcat tgagcattgc tccccactct ggtgtcggcg gctgcgcacc gaggccgaga
     9061 aatgacaggc tggcggcaga gataatcgag gtgccaatgc gcatggtgaa aaacaccacg
     9121 atagaagaga cggtcccagg caggatatga cgcaataaaa tggtcatatc gctggcacca
     9181 atactgcgtg ctgactcaat aaaggtttgc tgtttcaaca ccagcgtgtt gccgcgcacc
     9241 aggcgggcaa acgcggggat ggaaaaaatg gcgactgcga taatcacgtt agcaatgccg
     9301 cttcccaaca ccgcaacaac agcgatcgcc agtaaaatac ccgggaaggc aaacagcaca
     9361 tcgcaaatgc gcatgatcag ccgatcccac cagccttcat aatatccagc caacaagccc
     9421 agcaacgtcc ctatcgccac accgataaac acggcaaaca cgcccgccgc cagcgagatt
     9481 tgcgcaccaa ccaggacacg gctgaaaatg tcgcgcccca gtgaatcgac gccaaaccag
     9541 tgctgcaaag aaggtccgtt attcagattg tcataatcaa aataattttc ggcgtcatag
     9601 ggagcgatcc agcgtgcaaa aatggccacc acaatcaata aaataacgaa taatgcggcg
     9661 gtcatcgcca tatgctgacg gcgaaatcgt cgccagaatt catgccacgg tgtacgtacc
     9721 tggtcaggtt tgaccagtgg catggcgttt aacaccgcct gacgtcgcca gttaaatagt
     9781 cgcatcctta cttgtacctg atagccgggt taatggcggc gtaaagcaca tccaccacta
     9841 agttgataag aataaattcc agcgagaaaa gcagaatttc cgcctgaatc accgggtaat
     9901 cgcgcatttc tacagagtca acgagtaagc gtccaagtcc gggccagttg aaaactttct
     9961 ccaccacgat agaaccaccg agcaaaaagc caaactgtaa gcccatcatg gtcactaccg
    10021 ggatcatcgc gttgcgtagc ccgtgtttga ggacaaccca ggtttcgctc acccctttcg
    10081 ccctcgcggt acgcatataa tcttcgctta aaacgtcgac aaatgacgca cgggtaaagc
    10141 gcgccatcac ggcggccact gccgcgccga gcgtcaggga gggtaaaatg tagtgctgcc
    10201 aactgtctgc tcccacagta ggcagccagc ccagttcaac ggagaatacc tgaattaaaa
    10261 acatccccag cgcaaatgcc ggaaacgaga tccccgacac cgcaatggtc atactcaatc
    10321 gatccggcca acgattacgc cagacggcgg cgataattcc cgccgccata ccaaatatca
    10381 ccgcccagac catactggtt atggtcagcc atagcgttgg cataaagcgg ctggcaatct
    10441 catcggcaac cggacgacgc gacaccatcg acaggccaaa atccccctgc acagcattgc
    10501 tgatatagtg ccagaactgg tgatacagcg gctgatccaa ccccagctgc tgacgcacca
    10561 gttctataac ctgcgcatca gcttcgggcc cggcaatcaa tcgcgccgga tcgccgggca
    10621 gcatgtggac aaataaaaac accagcaccg agacgataaa cagcgtcgga atcaacccca
    10681 gtaatcgttt gataacgtaa taaagcattc cactccctgc gttgcttatt gcaaatccgc
    10741 gtcttcaaag ctgaagccgg tatctggcat gatccaaaaa ccggtcaggt ttttactgtg
    10801 tgccgacacc agtttttcta ccaccagcgg gatccacggc gattcttgcc agatgatatc
    10861 ctgcgccgcc ttatataagc gggtcttttc cgccggatca ttcgttttca gtgcctgagc
    10921 caggaagtca tccacctgtt tattgctgta aaacgcggta ttaaacagcg tcggtggcca
    10981 gttttgcgag gcaaacagcg gcgatagtgc ccagtcggct tcgccggttg aagccgacca
    11041 gccagtgtag aacatccgca cgccactctc tttttgccct ttaccttcaa cttctgccgc
    11101 ccgctgtccg gcatccatcg cggtcacctg ggctttaatc ccgacctgcg ctaactgctg
    11161 ctgggtaaat tgcagcactt tctgcgcggt gctgtggtta tgtgacgacc acagcgtggt
    11221 actgaaaccg ttgggatatc ccgcctcttt cagtaattcg cgcgctttca ctggatcgta
    11281 aggccacggt ttataacttt gcgcgtaggc gatacttggc ggtaccacac cagtagctgg
    11341 agttgcatag cccgcaaagg caactttcac cagcgccgga cggttaatgg cgtaattcag
    11401 cgcctcacgg accttcgggt tatcgaacgg cttttgcgtc acgttcatac tgatataacg
    11461 ctgcataatt gacggactgg ccatcaactc gatatttttg tttttctcca gcagtgtggc
    11521 ctgctcgtag ggaatgggga aagcaaactg cgcttcaccg gtttgcagca ttgccgcgcg
    11581 ggtgttgtta tccgccaccg gacgccaggt tatgctgtcc agtttgggca atcctggctg
    11641 ccagtaaccc gcgaattttt tcaccttcac aaaatcggtc tgattccagg tatccagttc
    11701 atacggtccg gttcccaccg gatgaaaacc aatctccttg ccatattttt ccagcgctgt
    11761 cggtgaaatc atcgcggtcg ccggatgggc aagaatatta ataaacgctg agaacggctg
    11821 tttgagggta atctttaccg ttgtcggatc gatcgcctcc gttttggcaa tattcttata
    11881 caggttatag cgtttaagat gattcgccgg atcgctggcc cggtccagat tcgcttttac
    11941 cgccactgcg ttgaaatcgg tgccatcctg gaatttaatt ccttcccgca atttcacggt
    12001 gtaagtaatg ccgtcatcgg aaacggtata actctccgcc agcacgtttt tcagtttcat
    12061 ctctttatcc agaccgaaca gcccctggta aaacgatttc gctacggcct gagataacgt
    12121 gtcattcgcg tcatacggat cgagcgtggt gaaattcgat cctaccgcca ccaccacatc
    12181 tttggcagcg aatgcacaag atgccatcaa cgctgtcgca atgcccagcg ccactaaccc
    12241 actacggtgt acagctcttg ccattttatt ctccgcctga atgttatcta cgcatgaatg
    12301 cgtattctga ttgtggttgt gcgacgtaat gccccggccc gacgcattgc aacgagacgg
    12361 ctgccacctc ttcgccacgc agatgaatat tgctgggaag atcgtccgac agcagtacac
    12421 gctgcggtcg ttgtcgggac ggttcagcga ccggaactgc cgccagtaat ttacgcgtat
    12481 aaggatgctg cgggttttcg aagaccgcgc accgtgaacc aatttcaaca atttgcccga
    12541 gatacatcac cgccacacga tgactaatcc gctctaccac agccatatcg tgggagataa
    12601 acagatacgc aatgccgaaa tcacgctgga gatcgagcaa caagttgata atctgcccgc
    12661 gaatagaaac atccagcgcc gaaacggctt cgtcggcaat gatcactttt ggattcaatg
    12721 ccaacgcgcg agcaatgcag atgcgctggc gctgaccgcc ggaaaactca tgcgggtaac
    12781 gccaggcatg ttcaggtaac aggcccacgc gctccagcaa ccacgcaacg cgtgcagccg
    12841 cgtctttacc tggcaataaa ccgtgtacac gcagcggttc gataatcgaa tcaccgatgg
    12901 tctgacgtgg gtccagcgaa gcgtaagggt cctgaaaaat aaactgaata tcccggcgca
    12961 atgcctgaag tttgccaggt gacaaggtat cgattcgctg accgttaaag ataatttcgc
    13021 cgccctgcga ttcgaccagg cgcagcaacg cccgcccggt agtggattta ccgctgccag
    13081 actcgcccac cagcgatagc gtttcgccag gccagagatc aaaactgact ttctcaacgg
    13141 catgcacttc ccgcgttacg cgattcaaca aaccgctgcg caaagggaaa cgggtgacaa
    13201 gattacgcac ccgtaaaaca ggttcgccat ccaccaccgt tttctgctcg atggggggtt
    13261 cctgtttcgc tggatgttca agcgatatca atgggaaacg tcggggataa tctaaccctt
    13321 tcatcgcacc aagttgcgga acagcagcta acagcgcacg ggtgtaagga tgttgcggtg
    13381 catgaaaaat ctgttcgacg gtacccgttt ccaccgcctc gccctgatac atcaccagta
    13441 cccgatcggc aatctctgcc accacgccca tatcgtgagt gataaagata acgcccatcg
    13501 acatctcttt ttgcaatact ttgattaatt gcaggatctg cgcctgaata gtgacatcca
    13561 gcgcggtggt tggctcatcg gcaatcagca ccgccgggcg gcatgacagc gccatcgcaa
    13621 tcatcactcg ctggcgcatc ccgccagaga gttgatgcga ataacgtgaa agaatggttt
    13681 gtgcctccgg aatgcgtacc tgatccagca tccgcttcgc ctcgaccatc gcttcttcac
    13741 gactggcgtt ctgatgcaga cgaattgatt cggcaatctg ttcacccaca gtaaatactg
    13801 ggttcagcga tgtcatcggc tcctgaaata tcatcgccat atccgcaccg cgcacatggc
    13861 gcatttgtgc agcgctctgc tcgctaagtt caatcacatc acgactgcgc cgccgcaaca
    13921 gcattttatc gcactgtact aaaccgcccg cctgttccaa cagacgcatc aatgccagcg
    13981 cagtcactga cttaccggag ccggattcgc ctacaattgc cagcgtctca ccgcgttgca
    14041 gactaaaaga gagattgcgg accgcagcta ttttctgctg gtcctgcata aaggcaatat
    14101 taagattttc aaccgccagc acattaccgg catcaagttc atcactgtgt ggcaacggtg
    14161 tccccttttt cacggtagat accggtggtt ggcgtatcgc ctgcgtagcc ccaggcgcga
    14221 tacattcctt cggtgttaaa cggtagcgcg acattccctt catggtcgat agcgattaag
    14281 ccaccgctac cgccaagcgc agggagtttt tccattacta cccgctcgca ggcttccgcg
    14341 agacttaatc cgccgtaatc cattaacgcg gcgatgtcat atgccgccag cgcgcggatg
    14401 aagacttcgc ctgtgccggt acaagaaacc gccacactgg cgttattggc gtagcatccg
    14461 gcacccacta agggactatc gccaactcgt ccgggtaatt tattggtcat tccgcctgtg
    14521 gacgtggctg ccgccagatt gccgtctaaa tccaacgcca cggcccccac ggtgcccatt
    14581 ttctgttttt catccagtgg cgcaccgcta tggtcgagga ctgttgcccc ttcctcgcgc
    14641 gctgccagta gttgttcata acgcaaaggc gtggagaaaa tctccggtga gacgcgctcc
    14701 atgccatgag caaacgcaaa attttctgcc ccttcgccaa tcatcatcac atgcgggctt
    14761 tgctccatca ccagccgggc ggcaagaacc ggattacgca gatgactaac gcccgccacc
    14821 gcaccggctt tcagggtgtt accatccatc acacaggcgt ccagttcatg ggtttcatca
    14881 cgcgtaaaga cagcgccaat tccggcgtta aacagtggac actcttccag cagacgcacc
    14941 gcttccgtca ccacatccag cgcactttcg ccggcttcca gcattttctg cccggtttca
    15001 acaatggcag acaacgcctc gatgtagcgt aattcctgtt gcagactcat ctgcgcgcgg
    15061 ctaattgcac ctgcgccacc atgaattgca atgactgctt tgcccatagt attcgtccat
    15121 taagggtggc tcgccacttt tgctataaat atcagaattt ttatgaatta ctcattcaat
    15181 ttttgaatat agaaagatgg atatggcatg taaaggcagg agccaacgct acgcccgagg
    15241 tggcatacag tctatggcgt ttttctgaca taataggcaa attcgatttt gcctccgcag
    15301 gagtgttttc atggaattta ccaccggatt gatgtcgctc gacaccgcgc ttaatgagat
    15361 gctttctcgc gttaccccac tgaccaccca ggaaacgctg ccactggttc agtgttttgg
    15421 tcgtattctg gcgagcgatg tcgtttcgcc actggatgtc ccggggtttg ataactccgc
    15481 tatggacggc tacgcggtgc gtttagccga tattgcctcc gggcaaccgc tgcccgttgc
    15541 cggtaaatcc tttgccggtc agccatacca tggtgaatgg cctgcgggta cctgcattcg
    15601 tattatgacc ggtgcgccgg tgccggaagg ctgcgaagcg gtggtgatgc aggagcagac
    15661 tgaacaaacg gacaatggcg tgcgttttac tgctgaagtg cgtagcgggc aaaatattcg
    15721 ccgtcgcggt gaagatatct ctgcaggtgc ggttgttttc ccggcgggaa ctcgcctgac
    15781 taccgcagag ctgccagtaa ttgcttcact ggggattgcc gaagttccgg tgattcgtaa
    15841 agtgcgtgta gcgctttttt ctaccggtga tgaactccag ttgcccggtc agccgctggg
    15901 cgacggccaa atctacgaca ccaaccgtct cgccgtacac ctgatgttag aacagctggg
    15961 ttgcgaggta atcaacttag ggattatccg cgacgatcca catgccctgc gcgccgcatt
    16021 tattgaagcc gacagccagg cggatgtagt gatcagttcc ggcggtgttt cagtgggtga
    16081 agcggattac accaaaatga ttcttgaaga gctgggggag atcgccttct ggaagctggc
    16141 gattaaacca ggtaaaccgt tcgcgttcgg taaactcagc aatagctggt tctgcggcct
    16201 gccgggcaac ccggtttcag cgacgctgac cttctatcaa ctggtacagc ctttgctggc
    16261 aaaactaagc ggcaacaccg ccagcggcct gcccgcgcgc cagcgcgtac gcacagcgtc
    16321 ccgcctgaaa aaaacgccag gacgtcttga tttccagcgc ggcgtgctgc aacgcaacgc
    16381 cgacggtgaa cttgaagtga cgaccaccgg acatcagggt tcacatatat ttagctcctt
    16441 tagtctcggc aactgcttta tcgtgctgga acgcgatcgc ggcaatgtgg acgtgcgcga
    16501 atgggtggaa gtagaaccgt ttaacgcgtt gttcggaggc ctgtaatggc ggaactcagc
    16561 gatcaggaga tgctgcgcta caaccggcaa atcattctgc gcggttttga ttttgacggt
    16621 caggaggcgc taaaagattc tcgtgtgctg gtagtcggcc tcggtggact cggctgcgca
    16681 gcttcgcagt atctggcaag cgccggtgtc ggcaacctga cgctgctcga cttcgacacg
    16741 gtttcgctct cgaatctgca acgccagaca ctgcacagtg atgccacggt cgggcaaccg
    16801 aaggtggaat ccgcccgtga cgccctgacg cggatcaacc cacatatcgc gattacgcca
    16861 gtcaatgcac tgctagatga cgcagaactt gcagcattga ttgctgaaca cgatctggtg
    16921 ctcgactgta cggataacgt tgcggtacgt aatcaactga acgcaggctg ttttgccgcg
    16981 aaggtgccgc tggtttccgg cgcggcaatt cgtatggaag ggcaaatcac ggtcttcact
    17041 tatcaggacg gcgaaccgtg ctatcgctgc cttagccgtt tgtttggcga gaacgcgtta
    17101 acctgcgtag aagcaggcgt gatggcaccg ttgataggcg taattggttc gttgcaggca
    17161 atggaagcga tcaaactgct ggcaggttat ggcaaaccgg ccagcgggaa aatcgtcatg
    17221 tacgatgcga tgacctgtca gtttcgcgaa atgaaactga tgcgcaatcc ggggtgtgag
    17281 gtgtgcgggc agtaattgcc acctccaagc ctgataagac gcgacaagcg tcgcatcagg
    17341 catttgctcc agacgccaga tgcggcgtga acgccttatc tggcctacgg ttcgggcaca
    17401 gatttccaag cgcggcaggc atcggtgcac tccgccacgt catcagcaat ttatgaggat
    17461 gcagaacgtc cggttaaatc gacgttctgc caaacgctcc ctgccagtcc tgctcaaact
    17521 tcgtcacagc ggcttccacc gccggatagc taatcatctg ttgtgccaca tccagtggca
    17581 gagtaattga ttcacatccg gccagtaagc agtccagcgc ctgacgcggg gttttgaaac
    17641 tcgctgccag cactttcgcc tgcggcgcat gcattttcaa taactggtgt aagtcggtca
    17701 cagtctgaat gccgctaccg ccctgagcat caatacgatt aacgtaaggc gcaacatatt
    17761 ccgcacctgc cagcgccgac agcagccctt gtgccgcgcc atataccgcg gttcccagcg
    17821 tcggaatccc ttccgctttt aacatcttaa tcgctgccag cccctcggct gtcaccggaa
    17881 ctttcaccac gatatccgca ataatagaac gcagcttaag cgcgtcattc accatccctt
    17941 cagcagtggt agccattacc tgggcaaaca gacgcccctg accgcccatc gcttcatgaa
    18001 gttgcggaag cacaacatcc agcggttttt tacccgcggc gataatgctt gggttagtgg
    18061 tcacacccgc cagcggaaaa atacgtgaca gcgccttcac cgcaacaacg tctgaagtat
    18121 ccagatacag ttccataacc atcctcaaaa tgtacttttt gtctgaatgt agactatcac
    18181 gggaaaactc tctgaacagt tgacgcacat caatctaact ttcattcgaa agtattttaa
    18241 tgtttgaacg aaacagagag gcaactatga ttttcaatat tcagcgctac tcgacccatg
    18301 atggccccgg tatccgcacg gtcgtatttc ttaaaggctg ttcgctgggc tgccgctggt
    18361 gtcagaaccc ggaaagccgc gcccgtacgc aggatctgct gtatgacgca cgactgtgtc
    18421 tggaaggctg cgagctgtgc gctaaggccg cgccggaagt gattgagcgc gcgctgaatg
    18481 gtttgcttat tcatcgggaa aagttaaccc cggagcatct gacggcgtta accgactgct
    18541 gtccgacaca ggcgttaacc gtgtgtggtg aagtgaaaag cgttgaggag atcatggcga
    18601 ccgttctgcg cgataaaccg ttttacgatc gcagcggcgg cggcttaacg ctttcgggtg
    18661 gtgaaccctt catgcagccg gaaatggcga cggcgctgct gcaagcctgc cacgaggcag
    18721 gcattcatac tgcggtagaa acctgtctgc atgtgccgtg gaaatatatc gccccttctc
    18781 tgccctatat cgatctgttt cttgccgatt taaaacacgt tgccgacgcg ccgtttaaac
    18841 agtggaccga cggtaacgcc gccagggtgc tggataacct gaaaaaactc gccgcagcgg
    18901 gcaaaaaaat cattatccgc gtgccgctga ttcagggctt taatgccgac gaaacctctg
    18961 taaaagccat taccgatttt gccgccgacg agctgcacgt tggcgaaatt cattttctgc
    19021 cctaccacac gctgggcatc aacaaatatc acttacttaa tctgccctat gacgccccgg
    19081 aaaaaccgct tgatgcgcca gagctgctcg attttgccca gcagtatgcc tgccagaaag
    19141 ggttaaccgc gaccttacga ggataacaac catgaccaca ctgaaactgg acacgctcag
    19201 cgaccgcatt aaagcgcaca aaaatgcgct ggtgcatatt gtgaagccgc cagtctgtac
    19261 cgagcgcgcg cagcactata ccgagatgta tcaacaacat ctcgataagc cgatcccggt
    19321 acgtcgcgcg ctggcactgg cgcatcacct ggcgaatcgc accatctgga tcaaacacga
    19381 tgagttgatc attggcaacc aggcaagcga agttcgcgcc gcgccgatct tcccggaata
    19441 tactgtctcg tggatcgaaa aagagattga tgatctggca gatcgtcccg gtgctggctt
    19501 tgcggtgagc gaagagaaca aacgtgttct gcatgaagtg tgcccgtggt ggcgcggtca
    19561 gacggtacag gatcgctgct atggcatgtt taccgatgag caaaaaggtc tgctggcgac
    19621 cggcatcatt aaagcggaag gcaatatgac ctccggcgat gcgcacctgg cggtcaattt
    19681 cccgctgctg ctggaaaaag ggcttgatgg tctgcgtgag aaagtagcgg aacgtcgctc
    19741 gcgcatcaat ctgacggtgc tggaagattt gcacggcgaa cagttcctga aagcgattga
    19801 tatcatgctg gtggcagtca gtgaacacat tgaacgtttc gctgccctgg cgcgtgcaat
    19861 ggccgcgacc gaaacccgcg aaagccgtcg cgatgaactg ctggcgatgg cagaaaactg
    19921 cgatcttatc gcccaccagc cgccgcagac tttctggcag gcgctgcaac tgtgttactt
    19981 catccagttg attttgcaga ttgaatctaa cggtcactcg gtgtcgtttg gtcgtatgga
    20041 tcagtatctc tacccgtact atcgccgcga cgttgaactg aaccagacgc tggatcgcga
    20101 acacgccatc gaattgctgc atagctgctg gttgaaattg ctggaagtga acaaaatccg
    20161 ctccggctcc cactcaaaag cctctgcggg aagtccgctg tatcagaacg tcaccattgg
    20221 cggacaaaat ctggttgatg gtcaaccaat ggacgcggtg aatccactct cttacgcgat
    20281 cctcgaatcc tgcggtcgcc tgcgttccac tcagcctaac ctcagcgtgc gttaccatgc
    20341 aggaatgagt aacgatttcc tcgacgcctg cgtacatgtg atccgctgcg gcttcgggat
    20401 gccggcattc aacaacgacg aaatcgtgat cccggaattt attaaactcg gtattgaacc
    20461 gcaggacgct tatgactacg cagcgattgg ttgtatagaa accgccgtcg gtggcaaatg
    20521 gggctatcgc tgtaccggca tgagctttat caacttcgcc cgcgtgatgc tggcggcgct
    20581 ggaaggcggg cgtgatgcca ccagcggcaa agtgttcctg ccacaagaaa aagcgttgtc
    20641 ggcaggtaac ttcaacaact tcgatgaagt gatggacgcg tgggatacgc aaatccgtta
    20701 ctacacgcgt aaatcaatcg aaatcgaata tgtcgtcgac accatgctgg aagagaacgt
    20761 gcacgatatt ctctgctcgg cgctggtgga tgactgtatt gagcgagcga aaagtatcaa
    20821 gcaaggcggc gcgaaatatg actgggtttc tggcctgcag gtcggtattg ccaacctcgg
    20881 caacagcctg gcggcagtga agaaactggt gtttgaacag ggcgcgattg gtcagcaaca
    20941 gctggctgcc gcactggcgg atgacttcga tggcctgact catgagcaac tgcgtcagcg
    21001 gctgattaac ggtgcgccga agtacggcaa cgacgatgat accgtcgata cgctgctggc
    21061 tcgcgcttat cagacctata tcgacgaact gaaacagtac cataacccac gctacggtcg
    21121 tggtccggtt ggcggcaact attacgcggg tacgtcgtct atctccgcta acgtaccgtt
    21181 tggcgcgcaa actatggcaa cgccggacgg gcgtaaagcc cacaccccgc tggcagaagg
    21241 cgctagtccg gcctctggta ctgaccatct cggccctact gcggtcattg gctcagtggg
    21301 taaactgcct acggcagcga ttctcggcgg cgtgttgctc aaccagaaac tgaatccggc
    21361 aacgctggag aacgaatctg acaagcagaa actgatgatc ctgctgcgta ccttctttga
    21421 agtgcataaa ggctggcata ttcagtacaa catcgtttcc cgcgaaacgc tgctggaggc
    21481 gaaaaaacat ccggatcagt accgcgatct ggtagtgcgt gtcgcgggtt attccgcctt
    21541 cttcaccgcg ctctctccag acgctcagga cgatatcatc gcccgtactg aacatatgct
    21601 gtaatctctt cggcccggct gcggcgtcgc cgggctaaat cattcacatc atcaatttca
    21661 tccttacttt cattcgaaat ataatttgtg ctctgcgtca cattgttatt acactgttac
    21721 ggtttcattt tcgagccagg agcacaccta tgagcgtaaa agttatcgtc acagatatgg
    21781 acggtacttt tcttaacgac gccaaaacgt acaaccaacc acgttttatg gcgcaatatc
    21841 aggaactgaa aaagcgcggc attaagttcg ttgtcgccag cggtaatcag tattaccagc
    21901 ttatttcgtt ctttcctgag ctaaaggatg agatctcttt tgtcgcggaa aacggcgcac
    21961 tggtttacga acatggcaag caactgttcc acggcgaact gacccgacat gaatcgcgga
    22021 ttgttattgg cgagttgcta aaagataagc aactcaattt tgtcgcctgc ggtctgcaaa
    22081 gtgcatatgt cagcgaaaat gcccccgaag catttgtcgc actgatggca aaacactacc
    22141 atcgcctgaa acctgtaaaa gattatcagg agattgacga cgtactgttc aagttttcgc
    22201 tcaacctgcc ggatgaacaa atcccgttag tgatcgacaa actacacata gcgctggatg
    22261 gcattatgaa acccgtcacc agtggttttg gctttatcga cctgattatt cccggtctac
    22321 ataaagcaaa cggtatttcg cggttactga aacgctggga tctgtcaccg caaaatgtgg
    22381 tagcgattgg cgacagcggt aacgatgcgg agatgctgaa aatggcgcgt tattcctttg
    22441 cgatgggcaa tgctgcggaa aacattaaac aaatcgcccg ttacgctacc gatgataata
    22501 atcatgaagg cgcgctgaat gtgattcagg ctgtgctgga taacacatcc ccttttaaca
    22561 gctgacctct ctaccggagc catgcgctcc ggttttccct tctttttaca tcctgtacat
    22621 cacatcaaat cattaaactt gcattaactt atttataact tgtattgtca catgcaaatt
    22681 agcatagttt cgaatgaaag ttaatgcaag gtggttatgg cttctacttt taccagcgac
    22741 acattgcctg ccgatcacaa agcagctatc cgtcagatga agcacgcgct gcgggcgcag
    22801 cttggcgatg tccagcagat ctttaatcag ctaagcgatg acattgccac gcgagtggct
    22861 gaaatcaacg cactcaaagc acagggcgat gccgtctggc cggtgctgtc ttatgccgat
    22921 atcaaagcag gtcatgttac tgcagagcag cgcgaacaga ttaaacgtcg cggttgtgcg
    22981 gtgataaaag gccatttccc ccgcgaacaa gcgctaggct gggatcagtc gatgctggac
    23041 tatctgaacc gcaaccgctt tgacgaggtc tacaaaggcc ccggcgataa tttcttcggg
    23101 acgctcagcg cttcacgtcc cgagatttac cccatctact ggtcgcaggc gcaaatgcag
    23161 gcccgccaga gtgaagaaat ggcgaatgcg cagtcgtttc tcaatcgtct gtggacattt
    23221 gaaagtgatg gaaagcaatg gtttaacccg gatgtgagcg tcatctaccc tgaccgtatc
    23281 cgccgccttc cgcccggaac gacctccaaa ggtcttggag cgcataccga ctccggggcg
    23341 ctggaacgct ggctgcttcc agcgtatcag cacgttttcg ccaacgtctt taatggcaat
    23401 ctggcgaaat acgatccctg gcatgcggca catcgtacgg aagttgaaga gtacacggtg
    23461 gacaacacca ccaaatgttc cgtgtttcgg acattccagg gctggactgc gctctcggat
    23521 atgctgcctg gtcaggggtt gctgcacgtc gtgcccattc ctgaagccat ggcgtacgta
    23581 ctgttacgtc cgctgcttga tgatgtgccg gaggatgaac tgtgcggcgt agcgcccgga
    23641 agagtgttgc cggtatcaga gcaatggcat ccactgttaa ttgaggcgtt aaccagcatt
    23701 ccaaaactcg aggccggaga ctccgtctgg tggcactgcg acgtcatcca ttccgttgcc
    23761 cccgttgaaa atcaacaggg ttggggcaac gtgatgtaca ttcctgcggc accgatgtgc
    23821 gagaaaaatc ttgcctacgc gcacaaggtg aaggccgcac tggaaaaagg cgcatcgccg
    23881 ggcgacttcc cgcgcgagga ctatgaaaca aactgggaag gacgctttac gcttgccgac
    23941 ctcaacattc acggtaagcg agcgttgggc atggatgttt gagagttaat ggaagtgaat
    24001 tgatgtggga ttaagaaaat cgattcgacg ttactcagat tgctgacaag tgcgctttct
    24061 taatgcctga tgcggcgtta acgccttatc cggcctacaa agtcctgcag attcaatata
    24121 ttacaggtac tgcgtaggcc tgataagcgt agcgcatcag gctattttgc atttgtcatc
    24181 agccgtcgtc accccgacag tatcggggtg acggataatc acttactcga tccctttact
    24241 acgcaggtaa tcttcgtaat taccgctaaa gtcgatcacg cgttccgggg tgatttccag
    24301 aatgcgggtc gccagggagc ttacgaactc acggtcgtgt gaaacaaaga tcagcgtgcc
    24361 ctgatacagt tccagcgcca tgttcagtga ctcgatggat tccatatcca ggtggttggt
    24421 cggttcgtcc atgatcagaa tgttcggctt ctgcatcatt aacttaccaa acagcatccg
    24481 ccctttttcc ccaccggaaa gcactttagc tggctttttg atgtcgtcct ggctgaacag
    24541 caaacgaccg agaatgctgc gtaccgcctg ctcgtcatcg ccttcctgct tccactgact
    24601 catccattcg aacacggtca gatcattttc aaactcatat tcgtggtcct gggcgtagta
    24661 accaatgcgc gcgttttcag accattttac ggtgccgctg tccggttgca gatcgcccac
    24721 cagcgttttc agcagcgttg atttaccgac gccgttggta cccagtaccg caagtttttc
    24781 accgacctcc agcagcaggt tgagattttt aaacagtgga ccgttatcaa accctttggt
    24841 cagaccttcc acttccagcg cgttacggaa cagtttctta tcctgttcaa aacggataaa
    24901 cgggttctga cggctggagg ctttcacctc ttccagtttg attttatcaa tctggcgcgc
    24961 gcgcgaagtt gcctggcgag atttcgaggc gttggcgcta aagcggctaa cgaaagattg
    25021 caactcagca atctgcgctt tcttcttggc gttatcggcc agcagacgtt cacgcgcttg
    25081 ggtcgccgcc gtcatgtact catcgtagtt acccggataa acgcgcagct cgccgtaatc
    25141 cagatccgcc atgtgggtac agaccatgtt aaggaagtga cggtcgtgcg agatgatgat
    25201 catggtgctg tcacgctcgt tcagcacctg ttccagccag cgaatggtgt cgatgtcgag
    25261 gttgttggtc ggttcgtcga gcaggagaat atccggctca gcaaacagcg cctgcgccag
    25321 aagcacacgc agcttccagc caggagcaac ttcactcatc gggccgtagt gctgttccac
    25381 tggaattccc acgccaagca acagttcacc ggcgcgagct tccgcagagt aaccgtccat
    25441 ttcgccgtat ttcacttcca gatcggccac tttatagccg tcttcttcac tcatttccgg
    25501 caaagcatag atgcggtcgc gctcctgctt cacttcccac aactctttat gccccatgat
    25561 caccgtatcc agcacagtga actcttcaaa ggcaaactga tcctgacgca gcttaccaat
    25621 gcgctcgttg ggatcgaggg aaacgttacc cagcgtcggc tcaaggtcgc cgccgagaat
    25681 cttcataaag gtggatttac cactaccgtt cgcgccaatc aggccgtaac ggttgccgcc
    25741 gccgaatttg acggaaatgt tttcaaacaa cggcttactg ccgaactgca tggtgacgtt
    25801 actggaaact aacacaggcg tatcctgaaa agagatatga caaaccgcgc attatgccat
    25861 aagtctgaaa gaagatcctg ctcctcgctt aaggtctaca ctcaagccaa aggcaaaaaa
    25921 tgtgatttcg tacacatctg atttcactgt gagctggaat gaacttataa tgcgcttcca
    25981 atactctaat attctcaacc caatggcctg ccaggcacaa aatctcgctt aacatgaata
    26041 tgaaattgaa aacattattc gcagcggcct tcgctgttgt cggcttttgc agtaccgcct
    26101 ctgcggtaac ttatcctctg ccaaccgacg ggagtcgcct ggttggtcag aatcaggtga
    26161 tcaccattcc tgaaggtaac actcagccgc tggagtattt tgccgcggag taccagatgg
    26221 ggctttccaa tatgatggaa gcgaacccgg gtgtggatac cttcctgccg aaaggcggta
    26281 ctgtactgaa cattccgcag cagctgatcc tgccggatac cgttcatgaa ggcatcgtca
    26341 ttaacagtgc agagatgcgt ctgtattact atccgaaagg gaccaacacc gttatcgtgc
    26401 tgccgatcgg cattggtcag ttaggcaaag atacgcctat caactggacc accaaagttg
    26461 agcataagaa agcaggcccg acctggacgc cgaccgccaa aatgcacgca gagtaccgcg
    26521 ctgcgggcga accgctaccg gctgtcgttc cggcaggtcc ggataacccg atgggtctgt
    26581 atgcactcta catcggtcgc ctgtatgcta tccatggcac caacgccaac ttcggtatcg
    26641 gcctgcgtgt aagtcatggt tgtgtgcgtc tgcgtaacga agacatcaaa ttcctgttcg
    26701 agaaagtacc ggtcggtacc cgcgtacagt ttattgatga gccggtaaaa gcgaccaccg
    26761 agccagacgg cagccgttat attgaagtcc ataacccgct gtctaccacc gaagcccagt
    26821 ttgaaggtca ggaaatcgtg ccaattaccc tgacgaagag cgtgcagaca gtgaccggtc
    26881 agccagatgt tgaccaggtt gttcttgatg aagcgatcaa aaatcgttcc gggatgccgg
    26941 ttcgcctgaa ttaatcgtca aaacgtaaaa caaaggcgga ctcataatcc gcctttttta
    27001 tttgccagac cttaccaggc cgggatcata actaacaaaa cgtatcccac cagcgccgcc
    27061 cacaacagca tcggtattga atagagatgg aaacgccacc agatgcggcg atcgttcgcc
    27121 atacgtagcg caatcaaatt tgccagcgat cctggtaata aaccaaagcc ccccacgttt
    27181 accgcccatg ccagtaataa agacggaggt acataattga gcagcaaaat ggtactcggc
    27241 acgttactga tcacctgcga taaaccgact gccgttaacc ataacccggg ttcagataaa
    27301 tgactcacgt tacccaacac gccttgcaac gctggaagct gggtcagtaa atggacgtcg
    27361 ataaacatcg ccataaacac cagcagcaac gtccagtcca cactcaacac cacgcgacgc
    27421 gccagcagca caaagcccgc cgccacaatc accagtcccc acagctcttg tttgaactcc
    27481 agcgccgtca gaaagacgat atacagcccc agacaattcc acaccagccg cggtttccac
    27541 tccggtgttt gcacccccgt atggtattgc agcgcctttc cagggaaaca acaccagcac
    27601 aacagcagga gtgtcagcat cattgcgcca gccatcggtg ccatttgggc aataaatccg
    27661 gcaaacgaaa gaccagaacg tccccagata agaatatttt gcgggttgcc aattggcgtc
    27721 agtagcgaac cagcgttgac tgccagcgcc tcaaaaataa tcagccgatt aaccgggatc
    27781 tcacacagtc tttttagcgt gatagtcagc ggaacaacaa taaacagcgc gacatcgttg
    27841 gtcagaaagg tagaaagcag cgccgccgcc agcaccataa acatcgccag ccgacgctcc
    27901 gtagcaaagc ggcgcaccat tttgcgcccc agcacatcaa aataaccgct taactccaca
    27961 cctttggtca gcagcatcag gccgcttaag gtgatgatgg tgtgccagtc gatagcagca
    28021 ggccaggatt tcggtgcaaa gggcacgaaa aagcttaatc cgataccaac aagaattaat
    28081 aactgaaaaa aacgatcgcc ttgcagcgtg cgtaaaaaag gcaggctcat ttggcaccgt
    28141 gtttctgggt aaacaaacga aaagcgtcga gcgtctcttc actaacatgg tgctccatgc
    28201 cttccgcgtc gcgacgggcg atttccggac tgacgcccaa caccaacaag aaattttcga
    28261 ctatctgatg tcgctcgcgg ctttcctgcg ccagcttctc tccttctgcc gttaaaaaca
    28321 cgccacgcca ggggatcatt tcaatcagcc ccatggttgc cagccgctta agcattttag
    28381 ccaccgtcgg ttgcgaaact cccagacgag cagccatgtc cacctgacga gcttccccca
    28441 cttccctgat caagtcagaa atcagctcaa cgtaatcatc aataagctcg cgccgatgcg
    28501 cctctctgac ctggcggaac ccttcaacgt gctcttccac gttcactaac tgcgtcactt
    28561 tttttgctat tggcgtacct gcgcgacgac tcattgtgct tcctcattat ggtgacgcat
    28621 acatagcgtt ctcatttcgt gcgcacattg taaaccagag ttgcgaaggt acaaaaaatt
    28681 aacgttttag caatagctat ataatatagc ctgtgctata tctgtatgta atgcaatcat
    28741 ccctcaagga tcgacgggat tagcaagtca ggaggtctta tgaatgagtt caagaggtgt
    28801 atgcgcgtgt ttagtcattc tccctttaaa gtacggttaa tgctgctctc tatgttgtgc
    28861 gatatggtca acaacaaacc gcagcaagat aaaccttcag ataaatagcg gcgtcgcggt
    28921 acgccgcttc actcctgctt ttatgcaggc ataacgcgtt ttggtctgaa aaaaaccact
    28981 ttttgtcgga tttgcaatcc ccttcgcaaa agatttgttc gtcagtagtt gacctgaacg
    29041 gcggctcgct ctatcttctt gcagccctgc gtatattgcg gctcgcggat gcggacccct
    29101 ttcccctctt cacgcactct tgcaggtatt gacccttgac gccagggtaa gcacatggcg
    29161 tttgttacga tagtggcata tgaatttaac cctcaaagaa tcgcttgtta cccgtagccg
    29221 ggtatttagc ccgtggactg cgttctactt tttacagtcg ctattaatta acctcggctt
    29281 aggttacccc ttcagtttgc tctacaccgc tgcgtttacg gctattttgc ttttgctatg
    29341 gcgaacattg cctcgcgtac aaaaagttct ggtcggtgtc agttcgctgg tggcggcttg
    29401 ttatttccct tttgctcagg cctacggcgc gcctaatttc aatacattgc tggcattgca
    29461 ctccaccaat atggaagagt cgaccgaaat cctgacgatt tttccgtggt acagctacct
    29521 ggtcggctta tttatttttg cgctcggcgt aatagcaatc aggcgaaaaa aagagaatga
    29581 aaaagcgcgc tggaatacct tcgacagcct gtgtctggta ttcagtgtgg cgacattttt
    29641 tgttgctccc gtgcaaaacc tggcctgggg tggcgtattt aaactgaaag atactggcta
    29701 tccggtattt cgttttgcta aggatgtcat cgtcaataat aacgaggtga ttgaagagca
    29761 agaacggatg gcaaaacttt ccggaatgaa agatacctgg acggtcactg ccgttaagcc
    29821 gaagtatcag acctatgtgg tagtgatcgg tgaaagcgcg cgtcgcgatg ccctcggtgc
    29881 ctttggcggt cactgggaca ataccccgtt tgccagcagc gttaacggtt tgatatttgc
    29941 tgactacatt gctgccagtg gctccacgca gaaatcgctt ggcttaacgc tcaatcgcgt
    30001 agtcgatggc aaaccacagt ttcaggataa ctttgtcacc ctggcaaatc gcgcgggctt
    30061 ccagacctgg tggttttcca accagggcca aatcggcgaa tacgatactg ctatcgccag
    30121 catcgccaaa cgtgcagatg aagtgtactt cctgaaagaa ggtaattttg aagcagataa
    30181 aaacacgaaa gacgaagcgt tactggatat gaccgctcaa gtgctggcgc aagagcactc
    30241 gcaaccgcag ctgattgttc tgcatctgat gggctcgcat ccgcaggcct gcgacaggac
    30301 acaaggcaaa tacgaaacct ttgtgcaatc gaaagaaacg tcgtgctatc tctataccat
    30361 gacgcaaacg gacgatttac tgcgcaagct gtacgatcag ttacgcaaca gcggcagcag
    30421 cttctctctg gtttactttt ctgaccacgg tctggccttt aaagagcgcg gcaaagacgt
    30481 gcaatacctt gcccatgatg ataaatatca gcaaaatttc caggtgcctt ttatggtcat
    30541 ttccagcggc gataaagcgc atcgggtgat taaagcccgc cgctcagcca atgacttctt
    30601 aggctttttc tcgcagtgga cgggaattaa agcgaaggaa ataaatatca aatacccgtt
    30661 tatatctgag aagaaagccg ggccgatata catcaccaac ttccagttac agaaggtgga
    30721 ttacaaccat ctcggaaccg atattttcga cccgaaacct taaaaacaaa aatccgcccc
    30781 gagaggcgga tttttatatc accaaagtga ttagaagcgg taaccaacac cggcaatcca
    30841 ggtgcctacg tcaacgctac gaatacggct ctgctcgtaa gagaagtcca gagcaacgtt
    30901 ttccatcggg ttgaactgca gaccagcacc gtaggagaaa ccgtagtcgc tggtgtcgtg
    30961 tttgtaggtc gggtattcag tggtctggaa tttaccataa cccacaccca ctacaccgta
    31021 gatgcttgcc cagtcgttaa tgcggtaagc cggaccagca gtgatgccgt agtactggtt
    31081 tttgttgtag tcaccagagc ttgcagtacg gcttttctcg gtgtaagtga aagaaccgat
    31141 cacacccagc gggctgttgt cttcttcata gcggtatttc aggttgaaac cgcccatttt
    31201 gttcatttgg ccctgagcgt cgctctgtgc gtaaccgcca gttacggtag aagtcgcagc
    31261 tacggaagta cctgcggtga aagccagaac tgcggccagt gctgaaagac atgcaatttt
    31321 tttcataacc acctcaaatg tgattcaaat aagtcctaag ttttaaatat atcaaaaatt
    31381 aatgggaaac tcttcgcgat ttgtgatgtc taacgggcca tttcatgtaa cagaacgttt
    31441 ccatacaccg ctatccatct aaatttaaat cactttttca gagaactgcg taagtattac
    31501 gcatgttttc cctgtcattc atccagatta ttcctaatca ccagactaat gattccatca
    31561 atcctggcgc attttagtca aaacggggga aaattttttc aacaaatgct caaccagcat
    31621 tgggtatatc cagtacactc cacgctttac ttaagtctag atatttgtgg gagaaaggat
    31681 gcctggttca ttacgtaaaa tgccggtctg gttaccaata gtcatattgc tcgttgccat
    31741 ggcgtctatt cagggtggag cctcgttagc taagtcactt tttcctctgg tgagcgcacc
    31801 gggtgtcact gcgctgcgtc tggcattagg aacgctgatc ctcatcgcgt tctttaagcc
    31861 atggcgactg cgctttgcca aagagcaacg gttaccgctg ttgttttacg gcgtttcgct
    31921 gggtgggatg aattatcttt tttatctttc tattcagaca gtaccgctgg gtattgcggt
    31981 ggcgctggag ttcaccggac cactggcggt ggcgctgttc tcttctcgtc gcccggtaga
    32041 tttcgtctgg gttgtgctgg cggttcttgg tctgtggttc ctgctaccgc tggggcaaga
    32101 cgtttcccat gtcgatttaa ccggctgtgc gctggcactg ggggccgggg cttgttgggc
    32161 tatttacatt ttaagtgggc aacgcgcagg agcggaacat ggccctgcga cggtagcaat
    32221 tggttcgttg attgcagcgt taattttcgt gccaattgga gcgcttcagg ctggtgaagc
    32281 actctggcac tggtcggtta ttccattggg tctggctgtc gttattctct cgaccgctct
    32341 gccttattcg ctggaaatga ttgccctcac ccgtttgcca acacggacat ttggtacgct
    32401 gatgagcatg gaaccggcgc tggctgccgt ttccgggatg attttcctcg gagaaacact
    32461 gacacccata cagctgctgg cgctcggcgc tatcatcgcc gcttcaatgg ggtctacgct
    32521 gacagtacgc aaagagagca aaataaaaga attagacatt aattaaattt acatttctgt
    32581 atggttatgc ataaccatgc agaatttctc gctacttttc ctctacaccg tatttatata
    32641 tcgaattatg caaaagcata tttattccga aaattcctgg cgagcagata aataagaatt
    32701 gttcttatca atatatctaa ctcattgaat ctttattagt tttgtttttc atgcttgtta
    32761 ccactattag tgtgatagga acagccagaa tagcggaaca catagccggt gctatactta
    32821 gtctcgttaa ttactgggac ataacatcaa gaggatatga aattatgagt accgctaaat
    32881 tagttaaatc aaaagcgacc aatctgcttt atacccgcaa cgatgtctcc gacagcgaga
    32941 aaaaagcaac agtagagttg ctgaatcgcc aggttatcca gtttattgat ctttctttga
    33001 ttaccaaaca agcgcactgg aacatgcgcg gcgctaactt cattgccgta catgaaatgc
    33061 tggatggctt ccgcaccgca ctgatcgatc atctggatac catggcagaa cgtgccgtgc
    33121 agctgggcgg tgtagctctg gggaccactc aagttatcaa cagcaaaacc ccgctgaaaa
    33181 gttacccgct ggacatccac aacgttcagg atcacctgaa agaactggct gaccgttacg
    33241 caatcgtcgc taatgacgta cgcaaagcga ttggcgaagc gaaagatgac gacaccgcag
    33301 atatcctgac cgccgcgtct cgcgacctgg ataaattcct gtggtttatc gagtctaaca
    33361 tcgaataaat ccatcgctga tggtgcagaa ctttagtacc cgataaaagc ggcttcctga
    33421 caggaggccg ttttgttttg cagcccacct caacgcactt atttagtgca tccatctgct
    33481 atccccagct gattaagtaa attttttgta tccacatcat cacacaatcg ttacataaag
    33541 attgtttttt catcaggttt tacgctaaat aatcactgtg ttgagtgcac aattttagcg
    33601 caccagattg gtgccccaga atggtgcatc ttcagggtat tgccctataa atcgtgcatc
    33661 acgtttttgc cgcatctcga aaaatcaatg agttgcaaaa ttggcacgat tttttcatat
    33721 atgtgaatgt cacgcagggg atcgtcccgt ggatagaaaa aaggaaatgc tatgaagtct
    33781 gtattaaaag tttcactggc tgcactgacc ctggcttttg cggtttcttc tcatgccgcg
    33841 gataaaaaat tagttgtcgc gacggatacc gccttcgttc cgtttgaatt taaacagggc
    33901 gataaatatg tgggctttga cgttgatctg tgggctgcca tcgctaaaga gctgaagctg
    33961 gattacgaac tgaagccgat ggatttcagt gggatcattc cggcactgca aaccaaaaac
    34021 gtcgatctgg cgctggcggg cattaccatc accgacgagc gtaaaaaagc gatcgatttc
    34081 tctgacggct actacaaaag cggcctgtta gtgatggtga aagctaacaa taacgatgtg
    34141 aaaagcgtga aagatctcga cgggaaagtg gttgctgtga agagcggtac tggctccgtt
    34201 gattacgcga aagcaaacat caaaactaaa gatctgcgtc agttcccgaa catcgataac
    34261 gcctatatgg aactgggcac caaccgcgca gacgccgttc tgcacgatac gccaaacatt
    34321 ctgtacttca tcaaaaccgc cggtaacggt cagttcaaag cggtaggtga ctctctggaa
    34381 gcgcagcaat acggtattgc gttcccgaaa ggtagcgacg agctgcgtga caaagtcaac
    34441 ggcgcgttga aaaccctgcg cgagaacgga acttacaacg aaatctacaa aaaatggttc
    34501 agtactgaac cgaaataata acgctacacc tgtaaagcgc actggcagtt ccctctcccc
    34561 cctggggaga ggattagggt gaggggcgca aacccgctcc ggggccatta attaccctga
    34621 atttgattat ttacaccacg gtaacaggaa caacatatgc agtttgactg gagtgccatc
    34681 tggcctgcca ttccgcttct gattgaaggt gccaaaatga ccctgtggat ttcggtcctc
    34741 ggtctggctg gcggtctggt aatcggattg ctggcaggtt ttgcacgcac cttcggtggt
    34801 tggatagcca accacgtcgc gctggtcttt attgaagtga tccgcggcac acctatcgtc
    34861 gtccaggtga tgtttatcta tttcgccctg ccgatggcgt ttaacgactt acgcatcgac
    34921 ccatttactg cggcggtggt caccatcatg atcaactccg gcgcgtacat tgcggaaatc
    34981 acgcgtggtg cggtgctgtc tatccacaaa ggttttcgtg aagcaggact ggcgctcggt
    35041 ctttcacgtt gggaaaccat tcgctacgtc attttaccgc tggcactgcg tcgtatgctg
    35101 ccgccgctgg gtaaccagtg gatcatcagc attaaagaca cctcgctgtt tattgttatc
    35161 ggcgtggcgg aactgacccg tcaggggcaa gaaattattg ccggtaactt ccgcgccctt
    35221 gagatctgga gcgccgtggc ggtgttctat ctgattatta ccctggtgct gagctttatt
    35281 ctgcgtcgtc tggaaagaag gatgaaaatc ctgtgattga atttaaaaac gtctccaagc
    35341 actttggccc aacccaggtg ctgcacaata tcgatttgaa cattgcccag ggcgaagtcg
    35401 tggtgattat cgggccgtcc ggttccggta aatcgaccct gctgcgctgc atcaacaaac
    35461 tggaagaaat cacctccggc gatctgattg tcgatggcct gaaggtcaac gatccgaaag
    35521 ttgacgagcg cctgattcgc caggaagcag gtatggtgtt ccagcagttt tacctcttcc
    35581 cgcatctgac ggcgctggga aacgtcatgt ttggtccgct acgcgtgcgt ggcgcgaaca
    35641 aagaagaggc ggaaaaactg gcgcgtgagc tgctggcgaa agtcggtctg gcagaacgtg
    35701 cacatcacta cccttccgaa ctttctggtg gtcaacagca gcgtgtggcg attgcccgcg
    35761 cgctggcggt gaagccgaaa atgatgctgt ttgatgaacc gacttccgct cttgacccgg
    35821 aactgcgcca tgaagtgctg aaggttatgc aggatctggc tgaagaaggg atgacgatgg
    35881 tgatcgtgac ccacgaaatc ggttttgccg agaaagtagc ttcgcggctg atctttatcg
    35941 acaaaggccg gattgcggaa gatggcaatc cgcaggtgtt gatcaagaac ccgccgagcc
    36001 agcgcttgca ggaatttttg cagcacgtct cttaataaga cacattgcct gatgcgctac
    36061 gcttatcagg cctacaggat atctggcaac ttattaaaat tgcatgaact tgtaggacgg
    36121 ataaggcgtt cacgccgcat ccggcaaaaa agcccgcacg ttgtcagcaa cctgcttaat
    36181 atcccttcct ccctttcacc cgaaagggag gcacaccaga ttcctctcat ttaaaatcgc
    36241 ccctccccca gcatctatac ttatcttttt gctctgtttt ctcactggag gagtcatgcg
    36301 gtggatcctg ttcatcctct tctgcctgct gggcgcacct gcccacgcgg tatccatacc
    36361 cggcgttaca accacaacga caacggactc aacgactgaa ccggccccgg aaccggatat
    36421 cgaacaaaaa aagcggccta tggcgcactg gcggatgtgc tggataacga cacctcgcgt
    36481 aaagagttga tcgaccagtt gcgtactgtc gccgcgacgc cccctgcgga accggtcccg
    36541 aagatcgtgc cgccgacgct ggttgaagag caaaccgtgc tgcaaaaggt caccgaagtc
    36601 agccgccatt atggtgaagc cctttccgcc cgcttcgggc aactttatcg caatatcacc
    36661 ggctccccgc ataagccatt taatccacaa accttcagca atgcgctgag ccatttttca
    36721 atgttagcgg tattagtgtt tggtttttac tggctgattc gcctgtgcgc actgccgcta
    36781 tatcgcaaaa tgggtcagtg ggcgaggcaa aaaaatcgtg agcgcagcaa ctggttgcag
    36841 cttccggcga tgattattgg ggcgtttatt atcgacctgc tgttactggc cctgacatta
    36901 tttgtcggcc aggtattaag cgacaacctg aatgcaggca gtcgcaccat cgctttccaa
    36961 caaagtttgt ttctcaacgc ctttgccctg attgaatttt tcaaagccgt actacgcctg
    37021 attttttgcc caaacgtggc ggagctgcgc ccgttcacga ttcaggacga gagcgcccgt
    37081 tactggggtc gtcgcctgag ctggttaagc agcctgattg gctatggcct gattgtggcc
    37141 gtgccgatta tctctaatca ggtgaatgta cagataggtg cgctggcgaa cgtcatcatt
    37201 atgctgtgca tgaccgtctg ggcgttgtac ctgatcttcc gaaataaaaa agagattacc
    37261 cagcatatgc tcaactttgc ggagcattcg ctggcttttt tcagcctgtt tatccgcgcc
    37321 tttgcgctgg tgtggcactg gctggcaagc gcctatttta tcatgctgtt tttcttttcg
    37381 ttgttcgatc cgggcaacag tctgaaattt atgatgggtg caacggtgcg cagcctggcg
    37441 attattggta tcgcagcgtt tgtttccggt atgttttccc gctggctggc gaaaaccatc
    37501 accctctcgc cacatactca gcgtaactat ccggagctac aaaaacggct gaatggctgg
    37561 ctttcggcgg cgctgaaaac ggcgcgtatt ctgaccgtct gcgtggcagt aatgttgctg
    37621 ttgagcgcat ggggattgtt cgatttctgg aactggctgc aaaacggcgc ggggcagaaa
    37681 accgtagata tcctgatccg catcgcactc attcttttct tctcggcggt tggctggacg
    37741 gtgctcgcca gtttgatcga aaaccggctg gcttcggata ttcatggccg cccgctaccc
    37801 agcgcccgta cgcgtaccct gctgacgctg ttccgtaacg cgctggcggt gattatcagt
    37861 accatcacca tcatgattgt gttgtcggaa atcggcgtca atatcgcgcc attgctggca
    37921 ggtgccgggg cattaggtct ggctatctcg tttggttcgc aaacgctggt aaaagatatt
    37981 atcaccgggg tatttattca gtttgaaaac ggcatgaaca ctggagattt ggtgactatc
    38041 gggccgttga ccggcacagt ggaacggatg tcgattcgct ccgtgggcgt gcgccaggat
    38101 accggggcgt atcacatcat tccgtggtct tcgataacca cctttgctaa cttcgtccgc
    38161 ggtattggtt cggtagtggc aaactacgat gttgatcgcc atgaagatgc tgataaagcc
    38221 aatcaggcac tgaaagatgc ggtaacggaa ttgatggaaa acgaagaaat tcgcgggctg
    38281 attattggtg aaccgaactt tgccgggatt gtcggcttaa gcaataccgc gtttacactg
    38341 cgtgtttcgt tcaccacgct gccactcaaa cagtggacgg tacgctttgc cctcgacagc
    38401 caggtgaaaa aacatttcga cctggcgggc gttcgcgcgc cagtgcagac ttatcaggtg
    38461 ctgcctgctc cgggcgcgac cccggctgag ccgctgccgc cgggggagcc aacgctttaa
    38521 cgttggcgat taacaaaacg gcggcgctgc tcgtcgttca taaaggtcca ggcaataaag
    38581 cgactctgct tttgcccctg ggccatctct ttttttacca ccttcaccgc gcccacgtcg
    38641 gtcagagcac gatacaacgg cggtaagttt tcaccacgag ataccagcga ggtaaaccac
    38701 atcacctgct tcgcgaagcc tttgctctct tcaatcatct ttttgataaa gttgacttca
    38761 ccgccttcac accacaactc ctgttgctga ccaccaaagt tcagtgcatc gtctttgttc
    38821 agccccaggt tacggcgttt acgctcacta cctgcccgcg ccgcagcggc ggaatcgtgg
    38881 aatggcgggt tacacaaggt cgcgtcgtat tgctcgtttt tgtggatgat gccgttaaaa
    38941 atcgccccac tctctttttg ccgacgcaga cgaatggcgc ggttaagccc cggattagca
    39001 ctgataatcg cctgcgcact gcttaacgcc tggctgctgg tttcactacc ggtaaaacgc
    39061 cagccatatt catgtacgcc aatcagcgga taaatacagt tcgcaccaac gccgatatcc
    39121 agaatgctgg cattcgctgg aattgttccg cttgcttcag ccagtaaatc ggcaaggtga
    39181 tgaatataat ccgcccggcc cggtactggt gggcagagaa aaccgtcggg gatatcccag
    39241 ttcgctacgg cgtaaaaatg ggccagcaac gccttattga gcgccttcac cgccagcgga
    39301 ttggcaaagt ctacgctttg ctcccctgcg ggtgtaagcg tgaggaattg cctgagttca
    39361 ggattgacct gacaaagcgt ggcgagatcg tagcggctgt gatgacggtt gcgcggatgc
    39421 aaccccggtt tctgggcgga catagcattc tcctttaagc agcggcgtaa gatacccgca
    39481 taacgcgaca gggtaaatat ctggctgcgt ttatcattcc ccaaaacctt aacatggcta
    39541 tggtttatgg caattcatca taaaaatcag cattatgcat gtcattgatt tgtcttattt
    39601 tgtaaaaata tatcgttcaa aaaataagca tttctctgtc aattttttgc gtcatttgca
    39661 tcgcgcaggc gacctacact tatcaggcac tacccgttgt atttatgagg atgtcattat
    39721 gaaaaagtgc ctcacactac tgattgccac cgtcctgagc ggaatctctc tcacggctta
    39781 tgccgcgcaa ccgatgagta acctggacag cggtcagcta cggcccgccg gtaccgtttc
    39841 ggcaactggc gcatcaaacc taagcgatct ggaggataaa ctggcagaaa aagcgcgcga
    39901 acaaggcgcg aaaggttatg tcattaattc cgcaggcgga aatgaccaga tgttaggtac
    39961 tgcaaccatc tacaaataac cccaccaacg taactgcatt cgcctctgga tgcagttttc
    40021 gcctgttttg taatcaaaaa acatcaaata tgaacgcaat gtaaataaat gtatttcttt
    40081 ttcgcgcaat gggtgataga aaatcgcatc aagtgataat gcttatcaaa attattatca
    40141 cttgcacgaa cactatcacg ggattaacag tggcatcgca tccgcagaga tgctttctcg
    40201 tggcagtgaa aatttcaaca tataagaaaa agtcacctgc aaaatggaaa acaatcgcaa
    40261 tttccctgcc agacaatttc attcgctcac gttctttgcc ggtctttgta ttggtattac
    40321 gcctgtggca caggcactcg ccgccgaagg gcaagctaac gcggatgaca cgctggttgt
    40381 cgaagcatca acgccttcgc tttatgcgcc acaacaatct gccgatccga aattctcgcg
    40441 tccggtagcg gatactaccc gcacgatgac ggtgatttct gaacaagtga ttaaagatca
    40501 gggcgcaacc aaccttaccg acgcgctcaa aaacgtcccc g
//
LOCUS       DAJTQQ010000028        39349 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000028 DAJTQQ010000000
VERSION     DAJTQQ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39349
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.028"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..158
                     /locus_tag="OO309_001625"
     CDS             <1..158
                     /locus_tag="OO309_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5846799.1"
                     /translation="IEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSG
                     GGVQELN"
     gene            387..977
                     /gene="uidR"
                     /locus_tag="OO309_001626"
     CDS             387..977
                     /gene="uidR"
                     /locus_tag="OO309_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR5846800.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1365..3068
                     /gene="uidA"
                     /locus_tag="OO309_001627"
     CDS             1365..3068
                     /gene="uidA"
                     /locus_tag="OO309_001627"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR5846801.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3074..4099
                     /gene="uidB"
                     /locus_tag="OO309_001628"
                     /pseudo
     CDS             3074..4099
                     /gene="uidB"
                     /locus_tag="OO309_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4138..5403
                     /gene="uidC"
                     /locus_tag="OO309_001629"
     CDS             4138..5403
                     /gene="uidC"
                     /locus_tag="OO309_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR5846802.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5629..7137)
                     /locus_tag="OO309_001630"
     CDS             complement(5629..7137)
                     /locus_tag="OO309_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5846803.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7238..8413)
                     /gene="manA"
                     /locus_tag="OO309_001631"
     CDS             complement(7238..8413)
                     /gene="manA"
                     /locus_tag="OO309_001631"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR5846804.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8612..10258
                     /gene="fumB"
                     /locus_tag="OO309_001632"
     CDS             8612..10258
                     /gene="fumB"
                     /locus_tag="OO309_001632"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5846805.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10401..11804
                     /gene="fumC"
                     /locus_tag="OO309_001633"
     CDS             10401..11804
                     /gene="fumC"
                     /locus_tag="OO309_001633"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR5846806.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11801..12730)
                     /gene="tus"
                     /locus_tag="OO309_001634"
     CDS             complement(11801..12730)
                     /gene="tus"
                     /locus_tag="OO309_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR5846807.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12806..14107)
                     /gene="rstB"
                     /locus_tag="OO309_001635"
     CDS             complement(12806..14107)
                     /gene="rstB"
                     /locus_tag="OO309_001635"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR5846808.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14286..15605)
                     /locus_tag="OO309_001636"
     CDS             complement(14286..15605)
                     /locus_tag="OO309_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCR5846809.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15830..16573)
                     /gene="rstA"
                     /locus_tag="OO309_001637"
     CDS             complement(15830..16573)
                     /gene="rstA"
                     /locus_tag="OO309_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR5846810.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16702..17037
                     /locus_tag="OO309_001638"
     CDS             16702..17037
                     /locus_tag="OO309_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR5846811.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17034..17756)
                     /gene="folM"
                     /locus_tag="OO309_001639"
     CDS             complement(17034..17756)
                     /gene="folM"
                     /locus_tag="OO309_001639"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR5846812.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17793..19175)
                     /locus_tag="OO309_001640"
     CDS             complement(17793..19175)
                     /locus_tag="OO309_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5846813.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19361..20305)
                     /locus_tag="OO309_001641"
     CDS             complement(19361..20305)
                     /locus_tag="OO309_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5846814.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20829..22361
                     /gene="pntA"
                     /locus_tag="OO309_001642"
     CDS             20829..22361
                     /gene="pntA"
                     /locus_tag="OO309_001642"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR5846815.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22372..23760
                     /gene="pntB"
                     /locus_tag="OO309_001643"
     CDS             22372..23760
                     /gene="pntB"
                     /locus_tag="OO309_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR5846816.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23785..24819)
                     /gene="tqsA"
                     /locus_tag="OO309_001644"
     CDS             complement(23785..24819)
                     /gene="tqsA"
                     /locus_tag="OO309_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR5846817.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25230..25595
                     /gene="mdtJ"
                     /locus_tag="OO309_001645"
     CDS             25230..25595
                     /gene="mdtJ"
                     /locus_tag="OO309_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR5846818.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25582..25911
                     /gene="mdtI"
                     /locus_tag="OO309_001646"
     CDS             25582..25911
                     /gene="mdtI"
                     /locus_tag="OO309_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR5846819.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25950..26771)
                     /locus_tag="OO309_001647"
     CDS             complement(25950..26771)
                     /locus_tag="OO309_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR5846820.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26871..26954)
                     /locus_tag="OO309_001648"
     CDS             complement(26871..26954)
                     /locus_tag="OO309_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846821.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27047..27382)
                     /locus_tag="OO309_001649"
     CDS             complement(27047..27382)
                     /locus_tag="OO309_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR5846822.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27779..29032)
                     /locus_tag="OO309_001650"
                     /pseudo
     CDS             complement(27779..29032)
                     /locus_tag="OO309_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29139..30050
                     /locus_tag="OO309_001651"
     CDS             29139..30050
                     /locus_tag="OO309_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5846823.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAKEPFVFFDPHVGTGLYDDILGL
                     MRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSE
                     MWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            30185..31405
                     /gene="mlc"
                     /locus_tag="OO309_001652"
                     /gene_synonym="dgsA"
     CDS             30185..31405
                     /gene="mlc"
                     /locus_tag="OO309_001652"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR5846824.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31530..32225
                     /gene="bioD"
                     /locus_tag="OO309_001653"
     CDS             31530..32225
                     /gene="bioD"
                     /locus_tag="OO309_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5846825.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32178..33470)
                     /gene="clcB"
                     /locus_tag="OO309_001654"
     CDS             complement(32178..33470)
                     /gene="clcB"
                     /locus_tag="OO309_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR5846826.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33629..34243)
                     /gene="dmsD"
                     /locus_tag="OO309_001655"
     CDS             complement(33629..34243)
                     /gene="dmsD"
                     /locus_tag="OO309_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR5846827.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34286..35140)
                     /locus_tag="OO309_001656"
     CDS             complement(34286..35140)
                     /locus_tag="OO309_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5846828.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35142..35759)
                     /gene="dmsB"
                     /locus_tag="OO309_001657"
     CDS             complement(35142..35759)
                     /gene="dmsB"
                     /locus_tag="OO309_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5846829.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35770..37808)
                     /locus_tag="OO309_001658"
                     /pseudo
     CDS             complement(35770..37808)
                     /locus_tag="OO309_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37869..38978)
                     /locus_tag="OO309_001659"
     CDS             complement(37869..38978)
                     /locus_tag="OO309_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5846830.1"
                     /translation="MINVSHIRTLSSIESTTSSPSIRLAMESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLEDLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
BASE COUNT        10007 a         9538 c        10065 g         9739 t
ORIGIN      
        1 aaattgaaca aaaaatgtta cagcacacgc cgatcagacg tctgggccaa ccgcaagata
       61 ttgctaacgc ggcgctgttc ctttgctcgc ctgcagccag ctgggtaagc ggacaaattc
      121 tcaccgtctc cggtggtggg gtacaggagc tcaattaata cactaacgaa ccggtaaacg
      181 gtcgtatgtg ttgtttaccg ggatagcgtg tgcaattcat caacgtctct gctaaacaac
      241 tggcagccaa atcacggcta ttggttaacc aattgctggg tgaaaagtat acgaatagag
      301 tgtaccttcg cactattcaa cagcaatgat aggcgctcac ctgacaacgc ggtaaactag
      361 ttattcacgc taactataat ggtttaatga tggataacat gcagactgaa gcacaaccga
      421 cacggacccg gatcctcaat gctgccagag agattttttc agaaaatgga tttcacagtg
      481 cctcgatgaa agccatctgt aaatcttgcg ccattagtcc tgggacgctc tatcaccatt
      541 tcatctccaa agaagccttg attcaggcga ttatcttaca ggaccaggag agggcgctgg
      601 ctcgtttccg ggaaccgatt gaagggattc attttgttga ctatatggtc gagtctattg
      661 tctctctcac ccatgaagcc tttgggcaac gggcgctggt ggtcgaaatt atggcggaag
      721 ggatgcgtaa cccacaggtc gccgccatgc ttaaaaataa gcatatgacg atcactgaat
      781 ttgttgccca gcggatgcgt gatgcccagc aaaaaggcga gataagccca gacatcaaca
      841 cggcaatgac ttcacgttta ctgctggatc tgacctacgg tgtactggcc gatatcgaag
      901 cggaagacct ggcgcgtgaa gcgtcgtttg ctcagggatt acgcgcgatg attggcggta
      961 tattaaccgc atcctgatcc tctcatttcg cgggctggtg ataactgtgc ccgcgtttca
     1021 tatcgtaatt tctctgtgca aaaattatcc tccccgtgtt cggagaattc ccccccaaaa
     1081 tattcaccgt agccatatgt catgagagtt tatcgttccc aacactctcg aacgaacgtt
     1141 cgattgctta ttttatggct tctgtcaacg ctgttttaaa gattaatgcg atccatatca
     1201 cgctgtgggt attgcagttt ttggtttttt gatcgcggtg tcagttcttt ttatttccat
     1261 ttctcttcca tgggtttctc acagataact gtgtgcaaca cagaattggt taactaatca
     1321 gattaaaggt tgaccagtat ttttatctta atgaggagtc ccttatgtta cgtcctgtag
     1381 aaaccccaac ccgtgaaatc aaaaaactcg acggcctgtg ggcattcagt ctggatcgcg
     1441 aaaactgtgg aattgatcag cgttggtgcg aaagcgcgtt acaagaaagc cgggcaattg
     1501 cagtgccagg cagttttaac gatcagttcg ccgatgcaga tattcgtaat tatgtgggca
     1561 acgtctggta tcagcgcgaa gtctttatac cgaaaggttg ggcaggccag cgtatcgtgc
     1621 tgcgtttcga tgcggtcact cattacggca aagtgtgggt caataatcag gaagtgatgg
     1681 agcatcaggg cggctatacg ccatttgaag ccgatgtcac gccgtatgtt attgccggga
     1741 aaagtgtacg tatcaccgtt tgtgtgaaca acgaactgaa ctggcagact atcccgccgg
     1801 gaatggtgat taccgacgaa aacggcaaga aaaagcagtc ttacttccat gatttcttta
     1861 actatgccgg gatccatcgc agcgtaatgc tctacaccac gccgaacacc tgggtggacg
     1921 atatcaccgt ggtgacgcat gtcgcgcaag actgtaacca cgcgtctgtt gactggcagg
     1981 tggtggccaa tggtgatatc agcgttgaac tgcgtgatgc ggatcaacag gtggttgcaa
     2041 ctggacaagg caccagcggg actttgcaag tggtgaatcc gcacctctgg caaccgggtg
     2101 aaggttatct ctatgaactg tgcgtcacag ccaaaagcca gacagagtgt gatatctacc
     2161 cgctgcgcgt cggcatccgg tcagtggcag tgaagggcga acagttcctg attaaccaca
     2221 aaccgttcta ctttactggc tttggtcgtc atgaagatgc ggatttgcga ggcaaaggat
     2281 tcgataacgt gctgatggtg cacgatcacg cattaatgga ctggattggg gccaactcct
     2341 accgtacctc gcattaccct tacgctgaag agatgctcga ctgggcagat gaacatggca
     2401 tcgtggtgat tgatgaaact gcagctgtcg gctttaacct ctctttaggc attggtttcg
     2461 aagcgggcaa caagccgaaa gaactgtaca gcgaagaggc tgtcaacggg gaaactcagc
     2521 aggcgcactt acaggcgatt aaagagctga tagcgcgtga caaaaaccac ccaagcgtgg
     2581 tgatgtggag tattgccaac gaaccggata cccgtccgca aggtgcacgg gaatatttcg
     2641 cgccactggc ggaagcaacg cgtaaactcg acccgacgcg tccgatcacc tgcgtcaatg
     2701 taatgttctg cgacgctcac accgatacca tcagcgatct ctttgatgtg ctgtgcctga
     2761 accgttatta cggttggtat gtccaaagcg gcgatttgga aacggcagag aaggtactgg
     2821 aaaaagaact gctggcctgg caggagaaac tgcatcagcc gattatcatc accgaatacg
     2881 gcgtggatac gttagccggc ctgcactcaa tgtacaccga catgtggagt gaagagtatc
     2941 agtgtgcatg gctggatatg tatcaccgcg tctttgatcg cgtcagcgcc gtcgtcggtg
     3001 aacaggtatg gaatttcgcc gattttgcga cctcgcaagg catattgcgc gttggcggta
     3061 acaattgacc tacatgggcc tcgggctttg ctacagcctg gtgaatattc cttatggttc
     3121 acttgctacc gcgatgaccc aacaaccaca atctcgcgcc cgtctgggcg cggctcgtgg
     3181 gattgccgct tcattgacct ttgtatgcct ggcatttctg ataggaccga gcattaagaa
     3241 ctccagcccg gaagagatgg tgtcggtata ccatttctgg acgattgtgc tggtgattgc
     3301 cggaatggtg ctttacttca tctgcttcaa atcgacgcgt gaaaatgtgg tacgtatcgt
     3361 ggcgcagccg tcattgaaga tcagtttgca aaccctgaaa cggaatcgcc cgctgtttat
     3421 gttgtgcatc ggtgcgctgt gtgtgctgat ttcgaccttc gcggtcagcg cctcgtcgtt
     3481 gttctacgtg cgctatgtgt taaatgatac cgggctgttc actgtgctgg tactggtgca
     3541 aaacctggtt ggtactgtgg catcggcacc gcttgtgccg gggatggtcg cgaggatcgg
     3601 taaaaagaat accttcctga ttggcgcttt gctgggaacc tgcggttatc tgctattctt
     3661 ctgggtttcc gtctggtcac tgccggtggc gttggttgcg ttggccatcg cttcaattgg
     3721 tcagggcgtt actatgactg tgatgtgggc actggaagct gataccgtag aatacggtga
     3781 atacctgacc ggcgtgcgaa ttgaagggct cacctattca ctattctcat ttacccgtaa
     3841 atgcggtcag gcaatcggag gttcaattcc tgcctttatt ttgggattaa gcggatatat
     3901 cgccaatcag gtgtaaacgc cggaagttat tatgggcatc cgcacatcaa ttgccttagt
     3961 accttgcgga tttatgctac tggcattcgt tattatctgg ttttatccgc tcacggataa
     4021 aaaattcaaa gaaatcgtgg ttgaaattga taatcgtaaa aaagtgcagc agcaattaat
     4081 cagcgatatc actaattaat attcaataaa aataatcaga acatcaaagg tgcaactatg
     4141 agaaaaatag tggccatggc cgttatttgc ctgacggctg cctctggcct tacctctgct
     4201 tacgcggcgc aactggctga cgatgaagcg ggactacgca tcagactgaa aaacgaattg
     4261 cgcagggctg ataagcccag tgctggcgcg ggaagagata tttacgcatg ggtacaggga
     4321 ggattgctcg atttcaatag tggttattat tccaatattg ttggcgttga aggcggagcg
     4381 tattatgttt ataaattagg tgctcgtgct gatatgagta cccggtggta tcttgatggt
     4441 gataaaagtt ttggctttgc cctgggggca gtaaaaataa aacccagtga aaatagcctg
     4501 cttaaattag gtcgcttcgg gacggattat agttatggta gcttacctta tcgtattccg
     4561 ttaatggttg gcagttcgca acgtacatta ccgacagttt ctgaaggagc attaggttat
     4621 tgggctttaa cgccaaatat tgatctgtgg ggaatgtggc gttcacgagt atttttatgg
     4681 actgattcaa caaccggtat tcgtgatgaa ggggtgtata acagccagac gggaaaatac
     4741 gataaacatc gcgcacgttc ttttttagcc gccagttggt atgatgatac cagtcgctat
     4801 tctctggggg catcggtaca gaaagatgtt tccaatcaga tacaaagtat tctcgagaaa
     4861 agcataccgc tcgacccgaa ttatacgttg aaaggggagt tgctcggctt ttacgcgcag
     4921 ctcgaaggtt taagtcgtaa taccagccag cccaatgaaa cggcgttggt tagtggacaa
     4981 ttgacctgga atgcgccgtg gggaagcgta tttggcagtg gtggttattt gcgccatgca
     5041 atgaatggtg ccgtggtgga taccgacatt ggctatccct tttcattaag tcttgatcgt
     5101 aaccgtgaag gaatgcagtc ctggcaatta ggtgccaact accgtgtaac tccgcaattt
     5161 acgctgacat ttgcaccgat tgtgactcgc ggctatgaat ccagtaaacg agatgtgcgg
     5221 attgaaggcg cgggtatctt aggtggtatg aactatcggg tcagcgaagg gccgttacaa
     5281 gggatgaatt tctttcttgc tgccgataaa gggcgggaaa agcgcgatgg cagtacgctg
     5341 ggcgatcgcc tgaattactg ggatgtgaaa atgagtattc agtatgactt tatgctgaag
     5401 taagaaataa cgccggagag aaaaacctcc ggcgtttcag attgttgaca aagtgcgcgt
     5461 tttttatgcc ggatgcggcg taaacgcctt atccagccta caaaaactca taaattcaaa
     5521 gtgttgcagg aaaaggtagg cctgataagc gtagcgcatc aggcaatctc tggtttgttt
     5581 tcagatgaaa acgccggagt gaaaattctc cggcgttttg gccgtgaatt actgctgcgg
     5641 aattgccggt acagccggaa cgttaagagc tggcatcgca aacatgccaa caaaatcttc
     5701 taacgacatt ttctgcccat ttaacgttat ctgaccgtta gcatattgca ggctggtggt
     5761 gatggtattg tcctgcaagg tggtcagacg gaacatctgc cccattgctg atgcaccttc
     5821 aacttgctgt ttcgccagtt ttttcgcttg atcttcctga taaccttcca gcttcgctac
     5881 ctgagtcatc aactcagttg ccatatccac cggaatggtc agtttcgcat ccagagattt
     5941 aaccgaacga tctacttcct gcgccagcgt ttgcggcgct tctttagtcg ttgccggatc
     6001 tttcaggaac agcgacagat tcagcgcact ttcaccctga ctgtttttcc agcttagcgg
     6061 cgcgatagta atcaccggat cgcctttcaa catcagcggc agggcgctaa agaaggcttc
     6121 cgtcactttc tcctgataaa gttcgggatt gttggcaatt tctggctgtg ccagcagcgc
     6181 ctgagtttgc gcgttatatt gctggctaaa ctgatgccag gcttcaccat caatctggcc
     6241 gacttttaaa gtcagcttgc cgctgcccag atcctgattc tgtaccttca ggctgtttag
     6301 cgagtaatcc agttggctat tgatcgtttt accgtcattg accagatccg atttaccgct
     6361 gatctccatg ccttccagca gtgccagttc tttgccttcc actgaaatgg tcattttttc
     6421 cagtgacagt ttttggtttc ctacgcgctc accaaaactt cccagcgtgc tggaaccgtc
     6481 ggttttcaga ttattaaagg tcaactgcac tttctggttg tattcgttaa ctgcgtctat
     6541 ccgaccactt tgcgcctctc cggaaaggga gatggctttg ccgtctctgt cagcatttaa
     6601 ctggaactcg ccgccgctaa aggcgacttt ttcatccttt tgctcgtaat tcagtggatt
     6661 gagcgaaata tcggaactgg aatcaccgct gtaaccaatg cgcgagttaa tctcaaaagg
     6721 cgtttcacct tttgccatat caaacagtgg tttgcttact tcgttattaa ccagcgtggt
     6781 ttgaattgat gccatcgacg ggatcaggtt cagtttttta agctgggcaa gcgggaaggg
     6841 accatgatca accgattcgt tgaagatgac gctctgaccg cttttaatcc acggattttc
     6901 tttcccggca atgggtttca ccaacagttg caactggctg ctgaatacgc cgcgatgata
     6961 gttttgataa ctcacttcca ggttggattc aggagctgtc agtttgagtt gcgcgttcgc
     7021 ctgcgcgacc atgtcttcga gatgggtttc aatcttcttg cctgtatacc atgcgccgcc
     7081 tgtccagact acgcctagcg caacaatgac gcctaccgct accagcgatt tattcataat
     7141 gattatccat aaaatgaaat caggcggact ggccgcctga aggtgttata agcctttaat
     7201 aagcttagca agagatgtta attttttcag taagctctta cagcttgttg taaacacgcg
     7261 ctaaacggcc gtggcctttg acagtcaccg gtgattcgtt ggcggcaata aacgctgatt
     7321 cacccggttt aagctgtaac tgctgagaac ctttccacaa cgttgcatcg ccttcgacgc
     7381 agaacaaaat ggcggcactc tgctggctga tggtggtttc tttatcacta aggtcatgca
     7441 gcgagaaggc aaaatcatcc acaggaatcg ggaagtccag ttctgcacct tgtttcaccg
     7501 gctgggtcaa caactggtta gccggtttgg cttcgaattt cacattggca accagttccg
     7561 gaatatcaat gtatttaggc gtcagacctg cacgcagcac gttatcggag ttcgccatca
     7621 cttccagcgc cacgccttgc aggtaagcgt gcggtgtttc agcgaacagg aacatcgctt
     7681 cgccaggatt caatttcacc acattcagta acagcgggga gaacaggccg ctgtcttccg
     7741 ggtaaaattc agaaattaaa cgaatcgttt gccacggttc accctgctga ctgtcgagcg
     7801 ccgattttaa aatcgccagc gcgcgggatt tttcttcacc ctgcatattc aacaggttgg
     7861 cgaacagttc gcttaaacgt tcggcatgtg gctgttgtaa aaagtgagca atcgccggat
     7921 gtgcaccggc gaccggctgg agtagggaga caatctcgga aaattcacga aacgcgttca
     7981 tcgcaaggaa aggcgtcagc gcaaaaacca gctccggctt gtggttagga tctttatagt
     8041 tacgctcggc ggcatccatc gggatacctg cggcattttc tttggcaaaa ccgatttcag
     8101 aattgtgttt gtttggatga acctgaatgg agagtggctg tgctgcgcat aatactttga
     8161 acaggaaagg cagttcgcca aagcgtttgg caacggcctc tccgagcaga gtcgatttat
     8221 cactctcaat cacatcacgc agtgaaacga tatctccggc ggcattctgc actcgtgaac
     8281 tgcttttcgg atgtgcgccc atccacagct cggccatcgg ctggctggac ggattttcca
     8341 taccataaag ttcagtcaac gccgttttgc taccccaggc atagttttgc actgagttaa
     8401 tgagtttttg catgatcaat ccctgtttta atgtggaaat taatcccgct attaaagcaa
     8461 gaatcctacg ggaagtaacc tggagccgca aaaagtcgta ctagtctcag tttttgttaa
     8521 aaaactgtgt aggatattgt tactcgcttt taacagggca acggaacacc cgcccagagc
     8581 ataaccaaac caggcagtaa gtgagagaac aatgtcaaac aaaccctttc attatcagtc
     8641 tccttttcca ctcaaaaaaa atgatactga gtattacctg ctaaccagtg aacacgttag
     8701 cgtatctgaa tttgaagggc aggagatttt gaaagtcgca cccgaagcgt taactctgtt
     8761 ggcgcgtcag gcgtttcatg atgcgtcgtt tatgctgcgt ccggctcacc aacaacaggt
     8821 ggccgacatt ctgcgtgacc cggaggccag cgaaaatgat aaatatgtgg cgctgcaatt
     8881 cctgcgtaac tccgacatcg cggcgaaagg cgttctgcca acctgtcagg ataccggcac
     8941 cgcgattatt gttggtaaaa aagggcagcg tgtatggacc ggtggtggtg atgaagcggc
     9001 gctggcgcgc ggtgtctata acacttatat cgaagataat ctgcgctact cgcaaaacgc
     9061 gccgctggat atgtataaag aagtgaatac cggcaccaat ctgccagcgc agatcgatct
     9121 ttatgccgtt gatggcgacg aatacaaatt cctctgtatc gcaaaaggtg gtggttcggc
     9181 aaacaagacg tatctctatc aggaaaccaa agcgttactg acgccgggga aactgaaaaa
     9241 ttacctggtt gagaagatgc gcacgctggg taccgcggcc tgtcctccgt atcatattgc
     9301 gttcgttatt ggtggaactt ctgcagaaac gaaccttaaa acggtgaaac tggcttccgc
     9361 gaaatactat gatgaactgc caacggaagg gaatgagcac ggtcaggcgt tccgcgatgt
     9421 ggaactggaa aaagaattac tgatcgaagc gcaaaatctc ggtctgggtg cgcagtttgg
     9481 tggtaaatac ttcgctcacg acatccgcgt gattcgcctg ccacgtcacg gcgcatcctg
     9541 tccggttggt atgggtgttt cctgctctgc tgaccgtaat atcaaagcga agatcaaccg
     9601 tcaggggatc tggatcgaaa aactggaaca taatccaggt aaatatatcc ctgaagagct
     9661 gcgtaaagcg ggagaaggcg aagcggtgcg cgttgacctt aaccgtccga tgaaagagat
     9721 cctcgcacag ttgtcgcagt atcccgtttc tacacgctta tcgcttaacg gcacgattat
     9781 cgttggtcgt gatattgctc acgccaaact gaaagagcgg atggataacg gtgaagggct
     9841 gccgcaatac attaaagatc atccgattta ctacgcgggt ccggccaaaa cgccggaagg
     9901 ttatgcctcc ggttctcttg gcccaacgac cgccggacgg atggattctt atgtcgatca
     9961 actgcaagcg cagggcggaa gtatgatcat gctggcgaaa ggcaaccgca gccagcaggt
    10021 gacggatgcc tgtaaaaaac acggcggctt ctaccttggc agtatcggtg gtccggccgc
    10081 tgtattggcg cagggaagta ttaagagcct ggaatgtgtt gaatatccgg aactgggaat
    10141 ggaagccatc tggaaaattg aagtggaaga tttcccggcg tttatccttg tggatgataa
    10201 aggaaatgac ttcttccagc agatacaact cacacagtgc actcgctgtg tgaaataaac
    10261 agagccgccc ttcggggcgg tttttttaca tggcacgaaa gaccaaacat ttgttatcaa
    10321 atagtaaata atgagtgagc taaaagttgc ttaacgaaag caaaacagaa agaaaaaatt
    10381 aatcaggtga ggagcaggcc atgaatacag tacgcagcga aaaagattcg atgggggcga
    10441 ttgatgtccc ggcagataag ctgtggggcg cacaaactca acgctcgctg gagcatttcc
    10501 gcatttcgac ggagaaaatg cccacctcac tgattcatgc gctggcgcta accaagcgcg
    10561 cagcggcaaa agttaatgaa gatttagact tgttgtctga agagaaagcg agcgccattc
    10621 ggcaggcggc ggatgaagta ctggcaggac agcatgacga cgaattcccg ctggctatct
    10681 ggcagaccgg ctccggcacg caaagtaaca tgaacatgaa cgaagtgctg gctaaccggg
    10741 ccagtgaatt actcggcggt gtgcgcggga tggaacgtaa agttcaccct aacgacgacg
    10801 tgaacaaaag ccaaagttcc aacgatgtct ttccgacggc gatgcacgtt gcggcgctgc
    10861 tggcactgcg caagcaactc attccgcagc ttaaaaccct gacacagacg ctgagtgaaa
    10921 aatcgcgtgc atttgccgat atcgtcaaaa tcggtcgaac ccacttgcag gacgccacgc
    10981 cgctaacact agggcaggag atttccggct gggtagcgat gctcgagcat aatctcaaac
    11041 atatcgaata cagcctgcct cacgtagcgg aactggctct tggcggtaca gcggtgggta
    11101 ctggactaaa tacccatccg gagtatgcgc gtcgcgtagc agatgaactg gcagtcatta
    11161 cctgtgcacc gtttgttacc gcgccgaaca aatttgaagc gcttgcgacc tgtgatgccc
    11221 tggttcaggc gcacggcgcg ttgaaagggt tggctgcgtc actgatgaaa atcgccaatg
    11281 atgtccgctg gctggcctct ggcccgcgct gcggaattgg tgaaatctca atcccggaaa
    11341 atgagccggg cagctcaatc atgccgggga aagtgaaccc aacccagtgt gaagcattaa
    11401 ccatgctctg ctgtcaggta atggggaacg acgtggcgat caacatgggt ggcgcttccg
    11461 gtaactttga actgaacgtc ttccgtccga tggtgatcca taatttcctg caatcggtgc
    11521 gcttgctggc agatggcatg gaaagtttta acaaacactg cgcagtgggt attgaaccga
    11581 atcgtgagcg aatcaatcaa ttactcaatg aatcgctgat gctggtgact gcgcttaaca
    11641 cccacattgg ttatgacaaa gccgcggaga tcgccaaaaa agcgcataaa gaagggctga
    11701 ccttaaaagc tgcggccctt gcgctggggt atcttagcga agccgagttt gacagctggg
    11761 tacggccaga acagatggtc ggcagtatga aagccgggcg ttaatctgca acatacaggt
    11821 gcagccgtgg aatgatcaaa cgaagcggct gcgcctgagg tttataacgg tgctgcacat
    11881 tgtcggcatc gtaatttaac aactcaccaa cgtccggcac gcccgcgcca ttatcccgat
    11941 taatcagtgc aatcagtggt gtagggcagg cgtgttggac ttgtttttga tctcctttgt
    12001 accagacgcg ggcaatcggc tgcaccttca ctggacgttt gatttttaac ttcgcgttct
    12061 gtggcagggc agcgatatcc tgatactctc gctccagttt tctttgccac tcctcgcgcg
    12121 tccacggtgc gacactgcgt ggtgatttca ggcttttttc cagctgtgcc aggacttcat
    12181 cacgatgcaa attcttaatg atatgtttat tagcccaacc aaagcgtaaa gtggcggggt
    12241 cgtgcagaac ggtgagcgtg cggtaagcat taagggtgat cagccccggc aaatgacgat
    12301 gcacccattc aaaacgtgcc gcggtgggga gttctgattc aaccgtgacg atatgctcga
    12361 acgtggtctt gagtttattg atgtgctgaa tatgactgac caacgctgct tgcgaaaggt
    12421 tatcgacctg gtaacacaac acgccaggca gacggacagc ggccttgctg ctgcgatttt
    12481 cggactgttg ttgaataaat aaatggcgga aatgacgcaa cgccagcgac tgcgcgtcgt
    12541 tgccgagatg ttgttttacc tcaatacgat taagcggatt atgctcatcc tcttttttta
    12601 cctccggcaa agagaacacg cgggcaacca atagcttgtg ttgctcaaga tgagcggcaa
    12661 atgtagccag ctcttgttcc atctggcgaa aggtagtgtt gagtcggtct acgagatcgt
    12721 aacgcgccat aatattaacc actttagtta caacatactt attttatagc acagtcgtgg
    12781 tgacttgacc atcgtgcgcg tagtgtcagg cagaggtaaa ttgcgggatg ttatgccata
    12841 acggccagct aaacgagaag cgagcaccac ccagttcgct gatgtcacaa ttaaccgtac
    12901 cgcccattgt cagtgctatt gagtggacaa ttgccagccc cagcccgcag ccgccggttg
    12961 agcgatcccg gctgggatcg aggcgaacaa aaggttcaaa gatatgttcg cggttttctg
    13021 gggcaatccc tgggccatca tcctcaacaa ttaatgtcgc tctattcccc gacagtagca
    13081 ggctggtttc aaccgttgaa tggcagtagc gcagggcgtt attgagcaaa ttatccagca
    13141 cgcgctccat taagcgcata tccaacgccg cataatggcc ttgcatgagc gtttttatcc
    13201 gtaccgtttt atcgggcgtt actgcctgaa tatctgccag atgcgttgac agccacaacg
    13261 gcaggtctgg ttcgctaaga tgaagctcgt tttgtgggcg atcgagtcgg gcataagtca
    13321 gcagctcttc aattaaagct tcaagttgac tgatatcacg attcaacgcc tgggattcgg
    13381 cggcgctcag gttatcgctc atctccagtc gataacgcag gcgcactaac ggtgttcgca
    13441 gttcgtgagc gataccgtca ataagctgtt ttttgctggc aattaaggcg ttgatattgt
    13501 ccgccatctg gttaaatgcg ataccaagtc gttcaaagct cgaaccctca tcaaagtgga
    13561 tacgttcatt gagatgccca tcgccaaatc gttgcgccgc tgcttccagt tttaacatat
    13621 cctgccagtg cggacgcatc cagataaaca ccggaaaggc gagggaaata gcaataaaag
    13681 cgatcagggc gatatccagc aatcgcattt gatggaggta ataaagataa ggaacaggac
    13741 caactgccag cacgtagtgg ctgcgcggga tacgctgcaa aaacgtgtac tgatcgtcca
    13801 gggcgacaat ttcgccgcca cgcagtcggt gcatggaaat atcatcaaga tggtatttac
    13861 tcagtggctc gacacgcaga tcgaaagaga gatttaaatc catctctttc agcgtcttac
    13921 cccagtcgtg tggggggatc tctcgcaatt cgctgcgcat cagatacagc gaactgttca
    13981 tcaaatcatc cagcgactgt ttgcccgcgc gttcggcggt aaatttgtac accagcccaa
    14041 ccagcagaga catcacaagg aagcagacaa acaataacag gtaaaactgg ataaacaatt
    14101 ttttcattgg catcgcccgc aacagaatac tattcgtatt aattcacgac tgtggagcaa
    14161 tgtaaaccgc caactggata agaccttgtt tggaagataa tgtccggaac agacggatgt
    14221 atccccggtg tattcatatt cagtaaattc aattttccac cggggcttgt ttattatgct
    14281 aaggtttaat ttaacgtgac gctttgttgt tctttagtct ctctatcctt aagaagacgg
    14341 gctgatatta caccggacat tattgaacca ttcacattaa gcgctgttct tgccatatca
    14401 acgatcggct ctatggcaat aaaaataact actaatgcga cagggaaatt aagtgttgaa
    14461 aggacaatca gcgccgcgaa tgtcccacca ctacctacgc ctgctacccc tattgagcca
    14521 agcgcgatgg caggtaaaag agttgcgagg aattgcaatg agaagggatc gatacccatt
    14581 gttggagcaa tcatcgccac catcacggca ggataaatcc cagcgcagcc attttgtccc
    14641 atgctggaac caaaagacgc tgcaatatta gcaatcgtat ttggaacccc caatttttcc
    14701 aatgcagaaa tagccagagg aattgaagcc gcactactgc gagaaacaaa cgcaaaggta
    14761 agtacttgcc agacggtctt gtaatagtta acggggtttt tacctattaa cagcaaaatg
    14821 cccgagtgaa caataaacat cacaaaaacg gccagataac aggcgacgat gaacccgagc
    14881 aaattggtaa actgtgaaag ctgatacgtt gagaagactt tcgccattag tgccattacc
    14941 ccataaggcg tcagcgcaat gacaatacgg accattttca ttacccatat ttgaatcgcg
    15001 ttgatccccg cgctcaattt ttcgccctct gctggtgcat ctttctttac ttttaacagc
    15061 gcaataccag caagaattgt aaagatgacg atgccaataa ccgaaacact tcttgccccg
    15121 gtcagatcaa ggaagaggtt ggttgggatc aatgacgtaa tgagtcgcgg taaagatacc
    15181 gcagctgttt tcgagacgtc atcgctggtt aaagaggatt gcatatgacc aaaggcgctg
    15241 gcatcaaggc caattgcact ggcaatagcc aggccaacta aacctgcaat catgactaaa
    15301 cacaacatac aggcaacaat agttaaagac atcttgccaa taccagcgct attttcgagc
    15361 ttattaatcg ctgaaagaat cgaaatgaat atgagtggaa cggcaacaag ttttaataaa
    15421 ttaacataac cgttaccagc aatattatac cattcgataa cggtttcact gcttgtctca
    15481 ggaatgaaat gcagggagat accaatgata gcccccagga aaatcgcgat aaaaacactt
    15541 ttggaaaggg attcgctctt ttgatccgtt ttgaaaatcc cccatgagca gatcaaaaaa
    15601 agcacaatat atgtgaaagt tgtaaccgtt ttttaagcct cttgcctcat tcagcacctg
    15661 aaatgccgga aagagtttgt tgtaaaaaat ctgcgccata agacgcaggg acgaggcgat
    15721 tatagagaaa ttttttctca ttttcatatc aaatgtaggg ggtttggaat ttgaataagc
    15781 tcttattaat tgaattcgtt aattgttctc tttttcagat gaacaaatat tagtcatctg
    15841 attgtttatt gtcactatcc catgcatgag gcgcaaaaag atagcctttg ttacgcacag
    15901 ttttaatgcg ataaggttct gcggcgttat cgagcagttt ttttcttaac cgcgaaatag
    15961 ccacgtccac gctacgatcc agtccgtcat aactgacgcc gcgtaaattt ttcagcaatg
    16021 catcgcggtc catgatttgc ccggcatgcg tagctagttc ccacaataat tcgaaatcag
    16081 ctgtcgagag cgagatttca gtgttagcca gggtgactac gcggttgatg gggtcgatag
    16141 ttaacgtgcc gaaatgcagg gctttgtagg gagtaagaga cgtttcctga agacctttgg
    16201 tcagtgtggc ttgctcattc tgacgcaaat gcaaacgtaa acgcgctagc aaaacagcag
    16261 ggggcgtcgt tttgagaata tagtcgcagg cacccatttc cagtgccagg atgtggttca
    16321 tatcgctatc gagagaggtt agaagaacaa tcggtccaga ccactttgcg cgtaaatcac
    16381 gacaaatggt catgccgtcc ttgcctggta gcatgatgtc gagtaacacc aaatccggat
    16441 tttctcgcaa aatggtttct tcggcctggt cgccgcgcgg ttctacggta acctgcatat
    16501 catgttttgc caggtacgcg gcaatcagtg aaccgacttc cgcatcatct tccacaaata
    16561 cgatagtgtt cataacattc acggtagata taaaaacgtc acaatacacc gcgctgtttt
    16621 tacttactat caatcgtttc taaacaagtt ttcatcaggt attctactga tgacttgtta
    16681 tggaagtgtt aaggtaaaaa gatggggctg gtaatcaaag ccgcgctcgg tgcgctggta
    16741 gtgctgttga ttggtgtttt agcaaaaacg aaaaattatt atatcgccgg gctgattcca
    16801 cttttcccga cctttgcgct tatcgcgcat tatattgttg ccagcgaacg cggcattgaa
    16861 gccttacgcg caaccatcat ttttagtatg tggtcgatta ttccctattt tgtttacctg
    16921 gtgtcgctgt ggtatttcac cggaatgatg cgcttgcccg ccgcttttgt tggttctgtt
    16981 gcgtgttggg ggataagcgc atgggtgttg attatatgct ggataaagct gcattaacgc
    17041 agatgacgac cgccatcaag tgggaaactg cgtccggtga caaagcaact ggtaagtaag
    17101 taatcgacca ggtcgatcac ttctttctcg ccaggcgcgg ttttcatcag tgatttattc
    17161 agcgcctgtt gtcgatattc ggcatcatca tgttcattaa acaggatcag cgatggcgca
    17221 atagaattca ctttcacttc cggtgccagc ttgcgggcaa acgagcgggt catattatcc
    17281 agtgccgctt tgcttgcagc atacgcaaca tgtttgtcgc taccgcgctc caccacataa
    17341 tcggtaaagt gaatgatatc gctggcggcg tgtccgtgcc cacgcagtaa tctttccagc
    17401 gcatggttga gcaggtatgg ggtattaacg tggatctgca tcatgcaagc cagtacgtcg
    17461 gccagtggcg cacccggttt ttccgcaatc cacgcactgg cgttatgcaa aatagcacgc
    17521 agaccatggg tggtttttag tacttcatcg gcaaacgcca tcacaccgtc gttggtcgaa
    17581 aaatcagcct gaatacactg cgcacctgca ttaatcagtc catcaatggc tggatagtgt
    17641 gtccgatagc tgacaatcac cggttgcttt tgattaatga aatgccatgc gagggcgagg
    17701 ccgatgcgac gacctccgcc agtaattaat attggcaagg gctgggtttt acccatcgtt
    17761 atctcctttg ctatccaacg atgggatgaa cgttatccca ccagcatcca ggtcgccgga
    17821 actgaggcaa ttaacagcat gccaatcagc accatttcct gacggttcag tacattatca
    17881 tgtgtatgcg ttttgcgcgc atacaggaaa accagcagcc ccggtgcata cagaacaacg
    17941 gataacagca agtgcattgg accagaggcg tacaataacc ataagccata aatgcaggca
    18001 ccgacaccta ccgctttatg cagtggacgg gttgcgattt tcagcaagaa cgcgccgacc
    18061 aggaaatagg gcaccagaat catttctgag gcgatggtca gcaacgtgtt gtaatcggaa
    18121 ccggtcagcc agatgagtac cagacaaatt tgcacgcaga tgttggtcaa ccacagtgag
    18181 gcggatggcg cagcttgtgc gttctggcgc gcaaaaatgc gtgggaatgc tttatgagtg
    18241 gcagccagga acggaacttc tgccgccatg atggtccagc ttaagtacgc gccgcaaacg
    18301 gaaacgatca aaccggcagc gatgatgatt tcgccccatg gtcccatcat ttccaccatc
    18361 agaccggcca ttgacgggtt acgaatttca gccagttcag gacgtgccac cacacccagc
    18421 gaaagcagcg ttaccagcaa gtaaacgccc agagcggaga gaaccgccag cagtgtcgct
    18481 ttgccaacat cacgtttatt acgcgcacgc gcagaaacaa ccacggcacc ttccacacca
    18541 atgaaaaccc acagggtgat cagcatggtg tttttcacct gttcccaaac aggtacgcca
    18601 agtgcaagtc cggtgaagtc gagcttgaag gtatccagtt tgaacatcat catcgccagc
    18661 acaacaaaca gacccagcgg caacaatttt gccagtgtcg ccaccaggtt aatgctggca
    18721 gcggtttgca ccccgcgcag gatcaaaaag tgaacaatcc acaataaagc cgatgcaccg
    18781 actatcgatt gccaggtatt accatcgcca aacaggcgca attccggcgt gtccgtaaaa
    18841 aagcttaacg cggaaaaaac gatcaccaga taggagacgt tggcgatgac cgcgcacagc
    18901 cagtatcccc atgcggaaca aaagccgatt agctcgccaa acccttcgcg ggcataggta
    18961 aagataccgc catcaagttc gggacgaatg cgcgtgagga tcagcatggc aaaggccagc
    19021 aataaaatgc cagcgccagt aataccccag ccgatgagca gtgctgccgg gctggcaact
    19081 gccgccatat tttgcggcag actgaaaaca cccgcgccca gcattgagct taataccagc
    19141 gcggtgagtg cgctcagtcc cagtttcttt tccatcgata tccagtgatt aaaacgtgat
    19201 aagaagaata aatattaggg caaaacgcat aaaaatggag tttgccactg aagcgcggga
    19261 ttttacgaag agatgtgtct gcatgcaatg gtcgcgggga aatttgtgac taaaaatgcg
    19321 aaaggcggca tcaagccgcc ttataggagt aactgaatag ttatttatac agatctgcgc
    19381 tgacggtcag gttattacca cgtttctgcc actggcgggt gatgtggtaa tacttagcac
    19441 ctttcttcgc ggcacgtttc gcaacctgat aggagacttc ggtcatgttg ccgtagttgc
    19501 cagagaattt gatgctgtcg aacgggacca tcatcgctgc tgtcgctttg ttcagttctt
    19561 cgactttagt gccatccggg agcgtgacgg tgtaacgccc gccttttgat gactgggttt
    19621 caaagaagcg accgacttca gaacttggtg atgcggtagt cgcaacaccc gggatctcaa
    19681 ctttcttcgc ggcttcgcca ccggcagcca gagctgcacg tcctgcttcg gaatctgccg
    19741 ggatcacatc cgggctctgg acgatacgtt tcttagcatc ttttttatag atgaatgcag
    19801 taatacgctg gttgccgccc tggttggcat cgatttgacg aacgatgtag aaagagtagg
    19861 cacctttcgc ttttgccgct ttggtgatgg catcattgac ttctggctgg ctacgataga
    19921 agccctggac ggtgaccgtg tcaaacggtt caatcagaac agcctgatct ttcggcagtt
    19981 cgacaacacc gttaattacg cgattacttg tttcttctgc tttttcagca tcggctttat
    20041 agaggtcagc gaccacacgc cagttaccgc tgttaccaaa atcagaagtg tcgacaacat
    20101 aaaaagaggc ggcaccttct ttatctgcgc gacgagaaac ggctttcacc gcttcgccaa
    20161 tagcattaaa acgaccggta accactacac ggtcaaaagg tttaaccgct gccgcttgct
    20221 ccggtgtcag ttctgttgct gcgttaacgg agaatgccgt agcagaaagc agtgccgacg
    20281 ccaggagggt gttcttaagc ttcataaaaa taatccttcg ccttgcgcaa accaggtact
    20341 ggtattgtta ttaacgagaa acgtggctga ttattgcatt taaacggtgt aactgtctgc
    20401 gtcatttttc atatcacatt ccttaaacca attttaatcc tgctcaaatg accgtctatg
    20461 cttaaaaaac agccgtatca gcatcattac tactgaagca actgaattgt ataagttaat
    20521 ttaatgttaa gtagtgattc gtgccggggc gatgtctcgt tttacccgac cgtcgaagac
    20581 aattatcagt ctttatccgg cgttctaagg tgtttatccc actatcacgg ctgaatcgtt
    20641 aatattttgc gagttcacgc cgaaatactg atttttggcg ctagatcaca ggcataattt
    20701 tcagtacgtt atagggcgtt tgttactaat ttattttaac ggagtaacat ttagctcgta
    20761 catgagcagc ttgtgtggct cctgacacag gcaaaccatc atcaataaaa ccgatggaag
    20821 ggaatatcat gcgaattggc ataccaagag aacggttaac caatgaaacc cgtgttgcag
    20881 caacgccaaa aacagtggaa cagctgctga aactgggttt taccgtcgcg gtagagagcg
    20941 gcgcgggtca actggcaagt tttgacgata aagcgtttgt gcaagcgggc gctgaaattg
    21001 tagaagggaa tagcgtctgg cagtcagaga tcattctgaa ggtcaatgcg ccgttagatg
    21061 atgaaattgc gttactgaat cccgggacaa cgctggtgag ttttatctgg cctgcgcaga
    21121 atccggaatt aatgcaaaaa cttgcggaac gtaacgtgac cgtgatggcg atggactctg
    21181 tgccgcgtat ctcacgcgca caatcgctgg acgcactaag ctcgatggcg aatatcgccg
    21241 gttatcgcgc cattgttgaa gcggcacatg aatttgggcg cttctttacc gggcaaatca
    21301 ctgcggccgg gaaagtgcca ccggcaaaag tgatggtgat tggtgccggt gttgcaggtt
    21361 tggccgccat tggcgcagca aacagtctcg gcgcgattgt gcgtgcattc gacacccgcc
    21421 cggaagtgaa agaacaagtt caaagtatgg gcgcggaatt cctcgagctg gattttaaag
    21481 aggaagcggg cagcggcgat ggctatgcca aagtgatgtc ggacgcgttc atcaaagcgg
    21541 aaatggaact ctttgccgcc caggcaaaag aggtcgatat cattgtcacc accgcgctta
    21601 ttccaggcaa accagcgccg aagctaatta cccgtgaaat ggttgactcc atgaaggcgg
    21661 gcagtgtgat tgtcgacctg gcagcccaaa acggtggcaa ctgtgaatac atcgtgccgg
    21721 gtgaaatctt cagtacggaa aatggtgtca aagtgattgg ttataccgat cttccgggcc
    21781 gtctgccgac gcaatcctca cagctttacg gtactaacct cgttaatctg ctgaaactgt
    21841 tgtgcaaaga gaaagacggc aatatcactg ttgattttga tgatgtggtg attcgcggcg
    21901 tgaccgtgat ccgtgcgggc gaaattacct ggccggcacc gccgattcag gtatcagctc
    21961 agccgcaggc ggcacaaaaa gcggcaccgg aagtgaaaac tgaggaaaaa tgtgcctgct
    22021 caccgtggcg taaatacgcg ttgatggcgc tggcaatcat cctgttcggt tggctgtcaa
    22081 gcgttgcgcc gaaagagttt cttgggcact tcaccgtttt cgcgctggcc tgcgttgtcg
    22141 gttattacgt ggtgtggaat gtatcgcacg cgctgcatac accgttgatg tcggttacca
    22201 acgcgatttc agggattatt gttgtcggag cactgttgca gattggccag ggaggctggg
    22261 ttagcttcct tagttttatc gcggtgctta tagccagcat taatattttc ggtggcttca
    22321 ccgtgactca gcgcatgctg aaaatgttcc gcaaaaatta aggggtaaca tatgtctgga
    22381 ggattagtta cagctgcata cattgttgcc gcgatcctgt ttatcttcag tctggccggt
    22441 ctttcgaaac atgaaacgtc tcgccagggt aacaacttcg gtatcgccgg gatggcgatt
    22501 gcgttaatcg caaccatttt tggaccggat acgggtaatg ttggctggat cttgctggcg
    22561 atggtcattg gtggggcaat tggtatccgg ctggcgaaga aagttgaaat gaccgaaatg
    22621 ccagaactgg tggcgatcct gcacagcttc gtgggtctgg cggcagtgct ggttggcttt
    22681 aacagttatc tgcatcatga cgcgggaatg gcaccgattc tggtcaatat tcacctgacg
    22741 gaagtgttcc tcggtatctt catcggggcg gtaacgttca cgggctcggt ggtggcgttc
    22801 ggcaaactgt gtggcaagat ttcgtctaaa ccgttgatgc tgccaaaccg tcacaaaatg
    22861 aacctggcgg ctctggtcgt ttccttcttg ctgctgattg tatttgttcg cacggacagc
    22921 gtcggcctgc aagtgctggc attgctgata atgaccgcca ttgcgctggt attcggctgg
    22981 catttagtcg cctccatcgg tggtgcagat atgccggtgg tggtgtcgat gctgaactcg
    23041 tactccggct gggcggctgc ggctgcgggc tttatgctca gcaacgacct gctgattgtg
    23101 accggtgcgc tggtcggttc ttcgggggct atcctttctt acattatgtg taaggcgatg
    23161 aaccgttcct ttatcagcgt tattgcgggt ggtttcggca ccgacggctc ttctactggc
    23221 gatgatcagg aagtgggtga gcaccgcgaa atcaccgcag aagagacagc ggaactgctg
    23281 aaaaactccc attcagtgat cattactccg gggtacggca tggcagtcgc gcaggcgcaa
    23341 tatcctgtcg ctgaaattac cgagaaactc cgcgctcgtg gtattaatgt gcgtttcggt
    23401 atccacccgg ttgcggggcg tttgcctgga catatgaacg tattgctggc tgaagcaaaa
    23461 gtaccgtatg acatcgtgct ggaaatggac gagatcaacg atgactttgc tgataccgat
    23521 accgtactgg tgattggtgc taacgatacg gttaacccgg cggcgcagga tgatccgaag
    23581 agtccgattg ctggtatgcc tgtgctggaa gtgtggaaag cgcagaacgt gattgtcttt
    23641 aaacgttcga tgaacactgg ctatgctggt gtgcaaaacc cgctgttctt caaggaaaac
    23701 acccacatgc tgtttggtga cgccaaagcc agcgtggatg caatcctgaa agctctgtaa
    23761 ccctgacggc ctctgcggag gccgtcactc tttattgaga tcgcttaaca gaacggcgat
    23821 gctttgacct cccgctgttt gttcaagcgc aattttgaca ataattgtca acggcacgga
    23881 aagcagcata cccaccggtc ctaacaacca tccccaaaaa atcaacgaca aaaataccac
    23941 caatgtcgaa agccccagcc cacgtcccat gatgcgcggc tccagaatat tgccgaagac
    24001 cagattaatc agcagatatc ccgccagcac cagcaacgct tcgtagaagc cattaaacac
    24061 cagtacctga gcgatagggg ggattgccgc gagaactgaa ccaatattag ggatgtaatt
    24121 aagcgcaaag gccagcaatc cccagacaaa agcgaagcga acatcgagtg cggcgagcat
    24181 cgcccaggcg accaggccgg tgatgatgct gatggctgtt ttcagcacca gataatgaga
    24241 aacactgtca atcgcacgtt gaatcgccgc catcccttca accggacgcg ccatcatttg
    24301 ctgaaatttt ccgggcaatt gtggcacttc gagcagcata aacagcaccg tcagcagcaa
    24361 taaaaatatt gatgacatgg cattagataa ctgcgtcaat aagttggtga gcaacgtcat
    24421 cgccgcgttc ggatcaatgt aatgcgccag ttggtcaact gagacgtcaa tccctacgcg
    24481 ttgcaacaac ggctcaagag cttgcagcgg cgtcataata gagttgcgat attgcggtaa
    24541 cgtccgcgtc aactcgttga gcgcggaacc cagataagct aatagcaaca ccatcgccat
    24601 cacgatgatt gtcatcaaaa tcgacactgc cagtacacgc ggcacacgcc agcggaccat
    24661 atgttgcacc agcgggttaa gaataacagc aataaataat gcgagaataa acggcacgat
    24721 gatctcggcg gcaaaacgga taccgcagag aataatgacc agcattccca acataatgac
    24781 gatttttagg ccattgagcg tgatgatcgg ctttgccatg cttgtccccg tttttcttcc
    24841 tttgctaata ataatgtctt tttcgctccg tcatcataac gaaaatcaaa ccgctcaggt
    24901 aaagaagtga aaatattttg agttaattct taagctatga tacaaatcag gcgtgttcaa
    24961 ctaccgagga caattatcat ctgcgatgac gagaagcaac actgcggata attgtaatat
    25021 tatggacaat ttgttcagga cgttattttc tacatttacc catcttcgta cctcatccac
    25081 tatcttgctc gtaggtgagc agcactggcg caacgcgctt tagtcctttc ttctgcctaa
    25141 gcttgcccta tgcgcagggc ttgaccactt tactttggtt tcgctgaatt aagcgaaaat
    25201 taaaatagtt ctcttgcagg agaaggacaa tgtatattta ttggatttta ttaggtctgg
    25261 ctattgctac agaaattacc ggtacgctgt caatgaaatg ggcgagcgtc agtgagggaa
    25321 atggcggctt tattttaatg ctggtgatga tttctctgtc gtatatattt ctctctttcg
    25381 ccgttaaaaa aatcgcctta ggcgtagctt atgcgctgtg ggaaggtatc ggtattttat
    25441 ttattacctt gtttagcgtt ttgttattcg acgaaagttt atcgctgatg aaaattgccg
    25501 ggttaaccac cctggtcgcc gggatagtgt tgatcaaatc aggtacccgt aaagcgcgta
    25561 aacctgaact ggaggtgaac catggcgcag tttgaatggg ttcacgccgc ctggctggca
    25621 ttggcaatcg tgctggaaat cgttgctaac gtctttttga aattttctga cggctttcgt
    25681 cgcaaaatat ttggcttgct ctccctggcg gcggtgctgg cggcctttag tgcgctttct
    25741 caggccgtta aagggatcga cttgtctgtc gtttatgcat tgtggggcgg gtttggtatt
    25801 gccgccacgt tagccgcagg ttggatcttg tttggtcaac ggttaaatcg taaaggctgg
    25861 attggcctgg tcttgctgtt ggctggaatg atcatggtga aacttgcctg atgaagacgc
    25921 tgcccgcgct gtcgggcagc gtttgaacat tatttttgcg acagttgatc cagcttgtcg
    25981 cgaaaaccgg taacagaaat ggcccggtta tcggcgcgcc agcgatcttt cgcggcagga
    26041 gccgaacttt gcaccccaat taattgccag ccgtcatcgg tatgcaacat cagaggcgaa
    26101 ccgctgtcac ccggcaaggt atcgcactga tgtgacatca ccgacgtttg cgcccagcca
    26161 gtcacttcac agttttgatg actgtataac gtatcgagat gatcttcagg gtatcccgcc
    26221 tgagtcactt tacgaccagc cgattttaat gcggcagtaa gcgcggcttt atctccctca
    26281 aataacggca acggtgtaat gccagaaggg ggattacgta gcacaatcaa tccgaagtcc
    26341 cacggtgcag ctgcgggagg aacaatccaa ccatccccat ctgcttttaa ccgctttccc
    26401 agtgtcggat caacgcggcc ttctatgtcg tggatctcat agcgccaaag acctttattt
    26461 gacacaaaac gcagcgctac cgccttatcc gctttaccct taggcggcgt caataaacag
    26521 tgtcctgccg ttaatgccag attgggtgca atcagcgtcg ccgtacataa attgccgctg
    26581 gccgtttcca gttgcccaac cgcatcccac ggtgattggg tcgtgtcatt cactggcaca
    26641 cgatcatcat gaccaaaaaa cagggtgctg acctcatcgt ttgccgattt ggcaacgtct
    26701 ggtttatctg caaacacaaa agcagacgtc aaactaattg cacccaacac tacagcaatg
    26761 gttgtacgca tatcacactc tggtgggtaa ttatgattat taaaagcaaa ccctcataaa
    26821 tactatagac gggacgggat gaaagtggga gtaaaatcag ataactacag tcaggaaaga
    26881 taaaaacggg cggcaatcag tgcgcataaa ataaggatga taagaatgaa ctcaaagcga
    26941 taacggccca ccatactacc ctccgctaaa ggcggtgcta aacgttaccc gcttagcacc
    27001 gtaattttca ggcgcgaggg ggcgcgccag cattactgtt gaaaacttac gctgcgggtt
    27061 gtgcagcagg ttttgccggt tgctgatggc cgtgtttccc ggcgtgtttc ttcgctgcct
    27121 gcgctttttg ttcaggggct ttctgttcag ctttcgtatt tttatgatgc tttttagccg
    27181 cctgcgcttt ctgggcaggt gctgctttat gctgtttttt atgatgtgta gttttcgctg
    27241 gcgctgcttt ggtggtcgtc gcagtcggag caggtgtggt cgcagtctct gcagcaaagg
    27301 cggcagaaga cagacccata gcagcggcaa caaccagagc taatactttt ttcattgtca
    27361 taccctcaat ttgttttttc atttaacccc actgcggggc cgttgaaata actatatccc
    27421 tgggaatggc agacttccgt gagtggttgg tttcagcgtg taacgatatg tacaaacgct
    27481 gaataaatta cagcgttgat aataacgtat tgtgacttaa ggaaaattta gctaccaata
    27541 atagtagtct tgatcgggtt aactattata ctacttacaa agcggtagaa taactgcgca
    27601 tcagtaataa atgacacaca gcaaaatgaa tccgtttatt tgggtactta taatcctgat
    27661 gacgctagat gcgctgcggg aattggctgg cgcttcgtct attttaggat ggctattaac
    27721 gttggtttga gcaggcaata agtccggacg ggtatttacc gcagtccgga cttatttttc
    27781 aggcgtgcag acgacgatgc aaacgcgtcc cgaccagcag agcaatgacc agcatcagag
    27841 caataaatgc gccgacgccg ttccagccat agttatgcca gaaaacacca cccagcgtcc
    27901 cggcaatact cgaccccaga tagtaactga acagatacag cgaggaggcc tggcctttag
    27961 cgcgttttgc gcgggggccg atccagctgc tggctactga gtgggctgcg aagaatccgg
    28021 ctgagaagag taacattccg gcaaagatca gccacagcga gctaaataag gtcatcagta
    28081 aaccaaacgg cataactccc gtcgaaaaca acatcaccgg accgcgccca tagcgggtgg
    28141 tcatggttcc ggctttgggt gagctccatg taccggtcaa ataagccagc gataataagc
    28201 caaccacggc ctgactgaca tgctaggggg aaagcatcaa ccgatagccg atgtaattaa
    28261 acagcgtgac gaacgacccc atcagcaaaa agccttctgc gaacaataac ggtaatcccc
    28321 ggtcacgcca gtgcagacga aagttgataa ataacgtctt agggcgcagc gaagtcgggc
    28381 aaaaatggcg tgattcaggg aggattttcc agaacatcaa cgccgaggcc agcgcgaaac
    28441 aaccgattgc cgccagagca attcgccagt tgaaaaagtc cgtgaagaca ccactaatta
    28501 agcgtccgct catgccgcca attgagttgc cgctgatata caaccccatc gaaaaggcca
    28561 cgaaactggg gtggatttcc tcgctaagat aagtcatgcc aacagctgcc acgccactta
    28621 acgaaagccc aatcaaggcg cgcataatca aaatgccgtg ccagctggtc atcattgtcg
    28681 aaagtaacgt acaaatggag gccaacagta gcgccgtgac catcactggt ttgcgaccaa
    28741 tggcatcgga tagcgggcca gtaaatagca aaccgatagc caacatcgcc gtggaaatgg
    28801 acagtgaaat actactgttc gcgggcgtta agccaaactc ctgcgaaagc accggaagga
    28861 taggctgcac acaatagaga agtgcaaagg ttgccagtcc ggcagagaac agcgccaggg
    28921 tgacgcgcat aaattgcggc gtaccgcgtt taataaattg atttggctga gaaatgcttt
    28981 gcttgtcagt gtcgcttgcc ggagcgccat caacagttgt agtacggctc acttgaaatc
    29041 cttgctaaat atgcctgtgg atcaggctta tacatagggt aggaaaatcg aattgttctg
    29101 tctaatatat taataatctc aaataagatg ttttaaatat gaatattgaa cttcgtcatc
    29161 tgcgttactt tgttgctgtt gcggaagagc tgcattttgg gcgcgcggct gcccgcctga
    29221 atatttcgca accgccgcta agtcagcaga ttcaggcact ggagcaacaa atcggtgccc
    29281 gactgctggc acgaactaat cgcagtgtat tgctgacggc agcaggaaaa cagtttcttg
    29341 cagatagtcg gcaaatcctg tctatggtgg atgacgctgc cgctcgcgct gaaagactgc
    29401 atcagggtga agcgggggag ttgcgtattg gttttacttc gtccgctcct tttattcggg
    29461 cggtgtccga tacgttatcg ctgtttcgcc gtgattatcc tgatgtccat ttacaaaccc
    29521 gcgaaatgaa cactcgcgag caaatcgctc cgctcattga aggaacgctg gatatgggat
    29581 tgctgcgtaa cacagcgtta ccggagacgc ttgaacacgc agtcatcgtc catgaaccgc
    29641 ttatggcgat gatcccgtac gatcatcccc tggcaaataa tccgagtgta acactggctg
    29701 aactggctga actggctgaa ctggcgaaag aaccctttgt cttttttgat ccgcacgtcg
    29761 ggactgggct gtatgacgat attctcggac tgatgcgacg ttaccatttg acgcccgtca
    29821 tcactcagga ggtgggcgag gcaatgacca tcatcggtct ggtttccgcc ggtctgggtg
    29881 tttcaatttt gcctgcgtca tttaaacgtg ttcagctcaa cgaaatgcgc tgggtgccga
    29941 ttgctgaaga ggatgcggtt tctgaaatgt ggttggtctg gccgaaacat catgaacaaa
    30001 gtccagctgc gcgtaacttt cgtattcatc tgctgaatgc tctcaggtga gggaaatttc
    30061 agcgaaaaag cccgaaaaat gtgctgttaa tcacatgcct aagtaaaaat ttgacgacac
    30121 gtattgaagt gcttcaccat agcctacaga ttatttcgga gcgcgaaaat atagggagta
    30181 tgcggtggtt gctgaaaacc agcctgggca cattgatcaa ataaagcaga ccaacgcggg
    30241 cgcggtttat cgcctgattg atcagcttgg tccagtctcg cgtatcgatc tttcccgtct
    30301 ggcgcagctg gctcctgcca gtatcactaa aattgtccgt gagatgctcg aagcacacct
    30361 ggtgcgagag ctggaaatca aagaagcggg gaaccgtggc cgtccggcgg tggggctggt
    30421 ggttgaaact gaagcctggc actatctttc tctgcgcatt agtcgcgggg agattttcct
    30481 tgctctgcgc gatctgagca gcaaactggt ggtggaagag tcgcaggaac tggcgttaaa
    30541 agatgacttg ccattgctgg atcgtatcat ttcccatatc gatcagtttt ttatccgcca
    30601 ccagaaaaaa cttgagcgtc taacttcgat tgccataacc ctgccgggaa ttattgatac
    30661 ggaaaatggt attgtacatc gcatgccgtt ctacgaggat gtaaaagaga tgccgctcgg
    30721 cgaggcgctg gagcagcata ccggcgtacc ggtttatatt cagcatgata tcagcgcatg
    30781 gacgatggca gaggccttgt ttggtgcctc acgcggggcg cgcgatgtga ttcaggtggt
    30841 tatcgatcac aacgtggggg tgggcgtcat taccgatggt catctgctac acgcaggcag
    30901 cagtagtctc gtggaaatag gccacacaca ggtcgaccca tatggtaaac gctgttattg
    30961 cgggaaccac ggctgcctcg aaaccatcgc cagtgtggac agtattcttg agctggcaca
    31021 gctgcgtctc aatcaatcca tgagctcgat gttacatggg caaccgttaa ccgtggactc
    31081 attgtgtcag gcggcattgc gcggcgatct actggcaaaa gacatcatta tcggggtggg
    31141 cgcgcatgta gggcgcattc ttgccatcat ggtgaattta tttaacccac aaaaaatact
    31201 gattggctca ccgttaagta aagcggcaga tatcctcttc ccggtcatct cagacagcat
    31261 ccgtcagcag gcccttcctg cgtatagtca gcacattagc gttgagagta ctcagttttc
    31321 taaccagggc acgatggcag gcgctgcact ggtaaaagac gcgatgtata acggttcttt
    31381 gttgattcgt ctgttgcagg gttaacattt tttaactgtt ctaccaaaat ttgcgctatc
    31441 tcaatttggg ccaggaaagc ataacttaga ctttcaaggt taattatttt cctagtttat
    31501 atttgtgaag cataacggtg gagttagtga tgctgaagcg tttctttatt accggtacag
    31561 acacttctgt agggaaaacg gtggtttccc gcgcattgct acaagcgtta gcctcccagg
    31621 gaaaaagggt tgcgggatat aaacccgtag cgaaggggag caaagagaca cccgaagggc
    31681 tgcgtaataa agatgccttg gtgttgcaga gtgtttcaac catcgaactg ccttatgaag
    31741 cagttaatcc tatcgcgtta agcgaagaag aaagtagcgt ggcgcacagt tgcccaatca
    31801 attacaccct catttcaaac ggcctggcaa acctgaccga taaagtcgat catgtcgtgg
    31861 tagaagggac tggcggctgg cgcagtctga tgaatgattt gcgtccactc tctgaatggg
    31921 tagtgcagga acaactgccg gtgttgatgg ttgtcggtat tcaggaaggt tgcattaacc
    31981 atgcactgct aacagctcag gcgatcgcca acgacgggct gccgctcatt ggctgggtgg
    32041 ctaaccgaat caacccagga ctggcgcatt atgcggaaat cattgatgtg ctgggtaaaa
    32101 aacttccggc accgctcatt ggtgaactgc cttatctgcc gcgcgccgaa cagcgtgaac
    32161 tggggcaata catccgctta gctatgctgc gcagtgtgct ggcggtagat agagtcacgg
    32221 tgtaacgtcc gcgaaattac cgacgcaatt acgcaggcaa tcaataaacc ggggagtagc
    32281 tgatactccc cggtcatttc acatatcatc aacgtcgaca taatcggcgc gtgcgtggtt
    32341 gccgccaaca gtgtcgccat cccggtcaat ccgagtaaaa gcgtaatttc ttcgccatca
    32401 gggaaccata atcccaggct acgaccatac aacatgccaa tggcaagacc gataaatagc
    32461 gtcggtgtaa agacaccacc gggtgcgccg gaaccgctac tcgccagcac ggcacacagt
    32521 ttacaaagga agatcccggc aatgatcatt aacagtggtg gggcggttaa aaaggattgt
    32581 acggtgctat agccgttgcc ccacactgca ggtgtgaaca gggaaagcag acccacgatc
    32641 aacccgccta gtgccagttg ccagggcggc gcaagtttga gactcacaaa tccacgatga
    32701 caggcgttca ttaacgttaa caacagtggt ccgcacagac ctgccagcac acctgtactg
    32761 ataatcagcg catagtcacg agcctgaacc gtcactgaga gttgtacgtt gtagagtaac
    32821 gcgtcgctat gattaatcag attgctaacc agccatgcca cgatggcgga aataatcacc
    32881 gggccgagag aggccaacat catagtgcca aacagcactt cggctataaa taaactgcca
    32941 gcaagcgggg cacgataggc cgcagccatt cccgccgcgg ccccacaggc gatccataat
    33001 ttccattcct ggcgtggcgt aaaacgttgg gcaaaacagg aggcggcaag ggcagctaaa
    33061 agaatcatcg caccttcgcg accaattgca ctgccgctgg ttactaccag cagcgaggca
    33121 agcgatttaa ccaggcttgc tgcgtaatcg aactgtccat cggtttgcaa cgcttccatg
    33181 taatcggtcg gcgcatgagg gcgttgttgg gtaaatttct gccagcccat cagcaacaaa
    33241 cccgccgcca gtccgccgag cgccggagtt agcagccgtc gccaggagga aaggtttgtc
    33301 gctgcattga ccagactgcc ggagtcatta ttgaggaaca accactccag tagcagcatc
    33361 gcatgacgaa acccggcaac ggcaaagaca gcgagaatac cgacgactgt tgcgataagc
    33421 agacggcgga acatagtgcg cagatccgga tagatgtgta gatgatgcat gagcagaaat
    33481 gggcaatgaa acacgggcga ctattttgcg gcaaaagagg ccaaccgcaa aagcagtgcg
    33541 tgctttaaga gataaaaaaa cgtgacattg taacgtatta ttgccgaatc gtgcgtaatc
    33601 gtcaccatcc ggcaatatta cggtgatcct atcgaaagag tggtttaacc gcgacaggaa
    33661 tcaacagttg tgattgccat tgcgccagcg ttaagcgagc cagttcaccc agtgcacggt
    33721 agaaagggtg ttctgctttt tcgataaaaa catcaagaaa acgtgttgac cacggaaaaa
    33781 ggtgccatgc cagcaattct tcgcattctg tctggcgacc attctccgcc aaccacgcag
    33841 ccatcaacag cagtgaacca aaatgatctt ccggttcgtt ttgcttcatt tcaaactgaa
    33901 tgcctttctc gcgcatccac tgacgaagtg ccaatgttga atcgccaaac agcacagatt
    33961 cgcgatccag ccagaccgac ccccacggcg gagacggcag tgcccacggg ccgacaaaca
    34021 aacgctgcca ggcctgggcg tgagtctctt cacactgtgt ctgaaaagcc gtcaccagcg
    34081 gcgctaatga cgcttctggt agaggccact gagtttccca gccgtcactg gtgagtaccg
    34141 caaccagagg tgcggcttcc gcactctctg gagcgtaata aaacagcgcg cccaacacgc
    34201 gcgccgcgac agaaaaatta tcttgctgtg aaaaatgggt cattcctaac atcctgaaat
    34261 gtgcgggttg ccccgcacca atttgttaac ctgcaatagc cattcctact gtcatgtgca
    34321 ggccataaaa gagcacacgc ccgatcatct caccaacaag tatgaggatt agccccagaa
    34381 caagtccggc aacgtgaggt tcacggcgac gaatgagcgg acaaagccag catccaagac
    34441 ctgcgcataa caataccaca cgccagacct gtaaggaggc gtaatctggt accagtgcgc
    34501 tggcttgttg cacggatgag tgaatagacg ccagagacaa accttgcagc acaatcacct
    34561 cggcacaagc gatcagcgcc agaacgctaa tgatggcaaa tggtgtggta ttaaatgtaa
    34621 cgcgggctgc ccgcagaatt gcggcagcaa gtattggacc gctcaacaat accgtcagga
    34681 agaaagccag cgtagtgtaa ccgttatgcc aggttggcac ggtgtcgatt tgatacacgc
    34741 aggtcatcat ccagacgaaa atgacgccga gcgccatgct gacaagtaac cagagtttcc
    34801 ccaacgcttg cggcatttta ccgatgaccg ccaccagcca ccatagtccg ccaacggcaa
    34861 aaaatatgga accggcagca atctcgttac tcagaccaga agctccgatt cgattcagcg
    34921 agttaaacgc tcgcagtggc gatcccaggt gcatgacaga ggcaataaac ccgatgccca
    34981 tcaacagcca gaggaaaaac atgccacgaa caatgtgctg acggtctgcg tcatttttgg
    35041 cggcgaacca gccaattccg ctaacaatca gcgcgcctac tacgcattga cccaatacgg
    35101 taaagataac cagtggccat tcatgccatc catttcccat tttacacttc ctccggatta
    35161 gccagataac cagtggtatc gccggtcggg cggctgttgg cgttaggttt gataacgata
    35221 ttgggttttg tgaaatgcgc gcgcggcagt ggcgcgacgg cagccagagt gccgtgcttc
    35281 tggcgcagct cttcaattgg accgaactca agtgcacgca gcgggcagga ttcgacgcat
    35341 atgggttgtt tcccctcggc gacgcgcgaa taacaaccat cgcacttcgt catgtgccct
    35401 ttttcagcat tgtactgtgg tgcgccgtac ggacaagcca tgtggcagta gcggcagccg
    35461 atacaaacat cttcatcaac caccacaaag ccatcttcac gcttatgcat tgcgccgctg
    35521 gggcagactt ttgtacatgc ggggtcatcg caatggttac aggagatgga gagataataa
    35581 gcgaacacgt tctggtgcca gatgccatta tcttcctgcc agtcgccgcc agcatattca
    35641 taaatacggc ggaaactgac ttccgggcca agatctttaa aatctttgca cgccagttcg
    35701 caggttttac agccagtaca gcggctggaa tcgataaaaa atccatattg tgtggtcatg
    35761 ggctactcct taaacctttt cgatctggac aagattgctg tgcgacgggt ttccctttgc
    35821 cagcggtgaa gggcggtgag aggtcagaat attgatactg ccgccgtgat cgacccggtc
    35881 accaaacata tccgctttaa gccacgcacc ttgcccgatg gcggtaacgc caggcagaat
    35941 acgcggagtc acttttgcgg caatcagcat ttctccatta ttgttaaata cccgcacggt
    36001 atcgccatga cggataccgc gtgcctgagc atcaatgggg ttgatccaca cctcttgtgg
    36061 gcaggcctgc tgtaacacat caatattgcc gtagctggag tgggtacgcg ctttggtttg
    36121 gcgacttcac tcagcatcca gtagatgggt ttgcgttcaa attttgctga ggttgcgggt
    36181 tgggcgagga tcacatagcc catattccct gcagattcat gagagataag gtcttcttgc
    36241 tctgttggca tcaggtcagg caacaggata tcgcaatact tagccgaggc cgtcatgaag
    36301 tggtcaatgc caacaatcat ctcgcacttg ctgtcatcct gaagcacctc atgggtgtga
    36361 ttgatgtcgc catgttgatt gatcaatgtg ttactggcgt agcaccataa aaacttgatg
    36421 gggacatcca gtttttcttt tccacgaaca ccatcacggg tcgcggtcat tttcgtacca
    36481 tggtcgatgg catctgtcca tgtaaaaacg gaaatctgcg ttttaacagg attctcgagc
    36541 atcgggaacc attctacccc cagatcccag ctaccttcgc gtacacctga gttgccgccg
    36601 tttatgccga cgttaccggt aagaacggaa agcatggcaa tagcgcggga cgtttgttcg
    36661 ccgttggagt gtcgttgtgg cccccaaccc tgacaaatat aagcaggttt tgctgaaccg
    36721 atctctcgtg ccaactggat aattttttct gccgggatgc tggtgatttt tgctgcccat
    36781 tccggcgttt tagctatgcc gtcaggccct tcgcccagaa tataggcttt ataatgcgcg
    36841 ttacgtggtg cgttggcggg cagcgttttt tcatcgtaac caacacaata tttgtcgaga
    36901 aatggctgat cgaccatgtt ttcagtaatc agtacccagg caatcgcaca ggccagtgcg
    36961 ccatcggtgc cagggcgaat gggcagccat tcatcttcac gcccggcagc tgtgtcgtta
    37021 tagcgtggat cgatgacgat catgcgtgcg tttgaacgtt cgcgggcttg ctcgacgtag
    37081 taagtgacac caccgccgct catccgcgtt tctgccgggt tatttccgaa cataacgacc
    37141 agtttcgtat tggcgatatc atccgggctg ttgccatcat tggcaccgaa catataactc
    37201 attgcggcac tgatctgtgc ggtactgtag ctgccatagc gactgagaaa acccccgcaa
    37261 gagttcatca gacggtacgg gacgttcgag ttggtgatat ttccgccatc tacgcctgtt
    37321 ccgtacagga catgtacagc ctcattgccg taatctttca ggatccgccg aagattatca
    37381 ctgatggtat ccagggcttc gtcccaactt atccgttcaa atttaccttc accgcgctcg
    37441 ccgacgcgct tcatgggata tttcaacctg tcaggatgat tcatccgtcg gcggatagag
    37501 cgcccgcgta aacatgcccg aacctgatga ttaccgtaaa cgtcgtcacc tgtcgtatca
    37561 gactccaccc agtacacggt gtcatctttc acatgcaaac gtagcagaca gcggctcccg
    37621 cagttaacgg tgcaggaact ccagaccgtt ttctcttcta ccggagcctc tgccgcccgg
    37681 accatttggg aaaatggcag agtgaaagca ctgcttgcca gcgcgagact gccaagtgcg
    37741 gaggttttca tcagacttct atggctgatt tcagccttca tgagcgcctc tgtggtatgg
    37801 attttcatca ttactcactt attgcttttc aaacaaaatg tcatgccaga atttatggtt
    37861 gtagtgggtt atattttttc gatctcgacc agattagtgt gctgcgggtt tcccttcgcc
    37921 agtggtgaag ggcgcagagt ggttagcgta ttcacacatc cgccatggtc gattttatcg
    37981 ccagacatat tgggctcgtg ccaggctccc tggcccatag cgctaactcc agggagaatg
    38041 cgtggtgtta ctttggctgg tagccgaact tcgccacgat ggttaaacac ccgcaccata
    38101 tcgccgttgg caatcccacg tttctgtgca tctatagggt tgatccacac ctcctgacgg
    38161 caggctgcct tcaggacatc aatgttgccg taggtcgagt gagtacggga tttgtaatgg
    38221 aaaccaaaca gttgcagtgg gaaggttcta cgttcagggg agtcccagcc ttcaaaggtt
    38281 gaggcataaa ctggcagtgg gcttatcact tcatcttttt ccagttccca ggtacgggca
    38341 atttccgcca gcttgctgga ataaatttca atcttaccgg aaggcgtttt aagtggattt
    38401 gcctcggggt cgtcacgaaa tgctttgtag gcgacaaaat ggccattggg atctttacgc
    38461 ttatagatac ccattttttt cagttcgtcg taagacggta acgccggatc tttggcaagc
    38521 attttggcgt acagatgttg taaccattgt tcctgcgtgc gaccttctgt gaacttttga
    38581 tagacgtcag gtccaagacg tttcgcgact tcactcagga tccagtaaat cggtttacgt
    38641 tcgaattttt cgctggtgac tggctggagg aaaatgagat atcccatgtt accggcgtag
    38701 tcgttaggaa taatatcttc ctgctcaacg gtcatcaggt cttccagcag aatgtcggca
    38761 tattttgccg atgaggtcat aaagttttcg atgaccacaa tcatttcgca tttcgattcg
    38821 tcctgcagaa tttcatgcgt tttgttgatg tcagaatgct gattaacgag ggtatttccc
    38881 gcgtagttcc agatggactc cattgccaga cggatggatg gacttgaagt agttgactca
    38941 atgctactta aggtcctgat atgactgaca ttaatcatga taacactgta tatccatcca
    39001 gttctttctc tgggtctccc tatcggtatc agcaagtcta cacctcaagc cctgacacaa
    39061 ggctgacaca aatcaatcta ctcagcgtag ctaagcctca gttagtccgc agagccaatg
    39121 gtcgatatac tatccgcttc agactcaagg gacagaccac accgtttctt tcagtatcga
    39181 cccgcagcac agacaggaga gtagccacaa tgcgtcagag agagcttgca gccaccgcta
    39241 aggccttcat gttggatagg cctgaagtct cactacagga gcttacagag catctccggt
    39301 ctatggctga gcagttcctg acagacgcta gtgacgatta ctggaatgg
//
LOCUS       DAJTQQ010000029        38648 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000029 DAJTQQ010000000
VERSION     DAJTQQ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.029"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..106)
                     /locus_tag="OO309_001660"
     CDS             complement(<1..106)
                     /locus_tag="OO309_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HCR5846831.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGE"
     gene            389..430
                     /gene="hisL"
                     /locus_tag="OO309_001661"
     CDS             389..430
                     /gene="hisL"
                     /locus_tag="OO309_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR5846832.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    406..529
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            576..1475
                     /gene="hisG"
                     /locus_tag="OO309_001662"
     CDS             576..1475
                     /gene="hisG"
                     /locus_tag="OO309_001662"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR5846833.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1481..2785
                     /gene="hisD"
                     /locus_tag="OO309_001663"
     CDS             1481..2785
                     /gene="hisD"
                     /locus_tag="OO309_001663"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR5846834.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2782..3852
                     /gene="hisC"
                     /locus_tag="OO309_001664"
     CDS             2782..3852
                     /gene="hisC"
                     /locus_tag="OO309_001664"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR5846835.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3852..4919
                     /gene="hisB"
                     /locus_tag="OO309_001665"
     CDS             3852..4919
                     /gene="hisB"
                     /locus_tag="OO309_001665"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR5846836.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            4919..5509
                     /gene="hisH"
                     /locus_tag="OO309_001666"
     CDS             4919..5509
                     /gene="hisH"
                     /locus_tag="OO309_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR5846837.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5509..6246
                     /gene="hisA"
                     /locus_tag="OO309_001667"
     CDS             5509..6246
                     /gene="hisA"
                     /locus_tag="OO309_001667"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR5846838.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6228..7004
                     /gene="hisF"
                     /locus_tag="OO309_001668"
     CDS             6228..7004
                     /gene="hisF"
                     /locus_tag="OO309_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR5846839.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            6998..7609
                     /locus_tag="OO309_001669"
     CDS             6998..7609
                     /locus_tag="OO309_001669"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR5846840.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7795..8772)
                     /gene="wzzB"
                     /locus_tag="OO309_001670"
     CDS             complement(7795..8772)
                     /gene="wzzB"
                     /locus_tag="OO309_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR5846841.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(8918..10038)
                     /gene="ugd"
                     /locus_tag="OO309_001671"
                     /pseudo
     CDS             complement(8918..10038)
                     /gene="ugd"
                     /locus_tag="OO309_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10288..11694)
                     /gene="gndA"
                     /locus_tag="OO309_001672"
     CDS             complement(10288..11694)
                     /gene="gndA"
                     /locus_tag="OO309_001672"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR5846842.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11803..12162)
                     /locus_tag="OO309_001673"
     CDS             complement(11803..12162)
                     /locus_tag="OO309_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846843.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12159..12665)
                     /locus_tag="OO309_001674"
     CDS             complement(12159..12665)
                     /locus_tag="OO309_001674"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846844.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12812..13375)
                     /locus_tag="OO309_001675"
     CDS             complement(12812..13375)
                     /locus_tag="OO309_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846845.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13582..14520)
                     /locus_tag="OO309_001676"
                     /pseudo
     CDS             complement(13582..14520)
                     /locus_tag="OO309_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14583..14957)
                     /locus_tag="OO309_001677"
     CDS             complement(14583..14957)
                     /locus_tag="OO309_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HCR5846846.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(14961..16109)
                     /gene="wzy"
                     /locus_tag="OO309_001678"
     CDS             complement(14961..16109)
                     /gene="wzy"
                     /locus_tag="OO309_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCR5846847.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16129..17034)
                     /gene="rfbG"
                     /locus_tag="OO309_001679"
     CDS             complement(16129..17034)
                     /gene="rfbG"
                     /locus_tag="OO309_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCR5846848.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17027..17917)
                     /locus_tag="OO309_001680"
     CDS             complement(17027..17917)
                     /locus_tag="OO309_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCR5846849.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(17947..19203)
                     /gene="wzx"
                     /locus_tag="OO309_001681"
     CDS             complement(17947..19203)
                     /gene="wzx"
                     /locus_tag="OO309_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCR5846850.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19219..19764)
                     /gene="rfbC"
                     /locus_tag="OO309_001682"
     CDS             complement(19219..19764)
                     /gene="rfbC"
                     /locus_tag="OO309_001682"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR5846851.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19769..20647)
                     /gene="rfbA"
                     /locus_tag="OO309_001683"
     CDS             complement(19769..20647)
                     /gene="rfbA"
                     /locus_tag="OO309_001683"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5846852.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20706..21605)
                     /gene="rfbD"
                     /locus_tag="OO309_001684"
     CDS             complement(20706..21605)
                     /gene="rfbD"
                     /locus_tag="OO309_001684"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR5846853.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21605..22690)
                     /gene="rfbB"
                     /locus_tag="OO309_001685"
     CDS             complement(21605..22690)
                     /gene="rfbB"
                     /locus_tag="OO309_001685"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5846854.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            complement(23063..23956)
                     /gene="galF"
                     /locus_tag="OO309_001686"
     CDS             complement(23063..23956)
                     /gene="galF"
                     /locus_tag="OO309_001686"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR5846855.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24131..25525)
                     /gene="wcaM"
                     /locus_tag="OO309_001687"
     CDS             complement(24131..25525)
                     /gene="wcaM"
                     /locus_tag="OO309_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR5846856.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25536..26756)
                     /gene="wcaL"
                     /locus_tag="OO309_001688"
     CDS             complement(25536..26756)
                     /gene="wcaL"
                     /locus_tag="OO309_001688"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR5846857.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26753..28033)
                     /gene="wcaK"
                     /locus_tag="OO309_001689"
     CDS             complement(26753..28033)
                     /gene="wcaK"
                     /locus_tag="OO309_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR5846858.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28213..29691)
                     /gene="wzxC"
                     /locus_tag="OO309_001690"
     CDS             complement(28213..29691)
                     /gene="wzxC"
                     /locus_tag="OO309_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR5846859.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29693..31087)
                     /gene="wcaJ"
                     /locus_tag="OO309_001691"
     CDS             complement(29693..31087)
                     /gene="wcaJ"
                     /locus_tag="OO309_001691"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR5846860.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31142..32512)
                     /gene="cpsG"
                     /locus_tag="OO309_001692"
                     /gene_synonym="manB"
     CDS             complement(31142..32512)
                     /gene="cpsG"
                     /locus_tag="OO309_001692"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR5846861.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32705..34141)
                     /gene="cpsB"
                     /locus_tag="OO309_001693"
     CDS             complement(32705..34141)
                     /gene="cpsB"
                     /locus_tag="OO309_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR5846862.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34144..35367)
                     /gene="wcaI"
                     /locus_tag="OO309_001694"
     CDS             complement(34144..35367)
                     /gene="wcaI"
                     /locus_tag="OO309_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR5846863.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35364..35843)
                     /gene="gmm"
                     /locus_tag="OO309_001695"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35364..35843)
                     /gene="gmm"
                     /locus_tag="OO309_001695"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR5846864.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35843..36811)
                     /gene="fcl"
                     /locus_tag="OO309_001696"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35843..36811)
                     /gene="fcl"
                     /locus_tag="OO309_001696"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR5846865.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36814..37935)
                     /gene="gmd"
                     /locus_tag="OO309_001697"
     CDS             complement(36814..37935)
                     /gene="gmd"
                     /locus_tag="OO309_001697"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR5846866.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(37961..38509)
                     /gene="wcaF"
                     /locus_tag="OO309_001698"
     CDS             complement(37961..38509)
                     /gene="wcaF"
                     /locus_tag="OO309_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR5846867.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38525..>38648)
                     /locus_tag="OO309_001699"
     CDS             complement(38525..>38648)
                     /locus_tag="OO309_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCR5846868.1"
                     /translation="CADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10323 a         9680 c         8697 g         9948 t
ORIGIN      
        1 cctctccatt ctgacgagta atgaggatcg gggcatgatc ttcaacggct ttcatcattg
       61 ttgccgacaa attctgacgc gcttcgctgt agctaattgt acgcatgtaa atctcctatt
      121 ttgtacagtt cattgtacaa tgataggtgt taattaacta tttattaatt agtttgtaga
      181 tcaagacatt gccagcgaga cgaaaaatca gactttgctc tttttggtgc tgtcaagtta
      241 gaggacagtc ctcttagccc cctcctttcc ccgctcattc attaaacaaa tccatcgcca
      301 taaaatatat aaaaaagccc ttgctttcta acgtgaaagt ggtttaggtt aaaagacatc
      361 agttgaataa acattcacag agatttttat gacacgcgtt caatttaaac accaccatca
      421 tcctgactag tctttcaggc gatgtgtgct ggaagacatt cagatcttcc agtggtgcat
      481 gaacgcatga gaaagccccc ggaagatcac cttccggggg cttttttatt gcgcggttgt
      541 taacggttca gacaggttta aagaggaata agaaaatgac agacaacact cgtttacgca
      601 tagctatgca gaaatccggc cgtttaagtg atgactcacg cgaattactg gcgcgctgtg
      661 gcattaaaat caatcttcac acccagcgcc tgatcgcgat ggcagaaaac atgccgattg
      721 atattctgcg cgtgcgtgac gatgacattc cctgtctggt aatggacggc gtggtagacc
      781 ttgggattat cggcgaaaac gtgctggaag aagagctgct taaccgccgc gcccagggtg
      841 aagatccgcg ctactttacc ctgcgtcgtc tggatttcgg cggctgccgc ctttcgctgg
      901 caacgccggt tgatgaagcc tgggacggtc cgctctcctt aaacggtaaa cgtatcgcca
      961 cctcttatcc tcacctgctc aagcgttatc tcgaccagaa aggtatctct tttaaatcct
     1021 gcttactgaa cggttctgtc gaagttgcgc cgcgtgccgg gctggcggat gcgatttgcg
     1081 atctggtttc caccggtgcc acgctggaag ctaacggcct gcgcgaagtc gaagttatct
     1141 accgctcgaa agcctgcctg atccagcgcg atggcgaaat ggaagaatct aaacagcaac
     1201 tgatcgacaa gctactgacc cgtattcagg gtgtgatcca ggcgcgcgaa tcaaaataca
     1261 tcatgatgca cgcaccgacc gaacgcctgg atgaagtcat cgccctgctg ccaggtgccg
     1321 aacgcccaac cattctgccg ctggcgggtg atcaacagcg cgtagcgatg cacatggtga
     1381 gtagcgaaac cctgttctgg gaaacgatgg aaaaactgaa agcactgggt gccagttcaa
     1441 tcctggtcct gccgattgag aagatgatgg agtgatcgcc atgagcttta acacaatcat
     1501 tgactggaat agctgtactg cggagcaaca acgccagctg ttaatgcgcc cggcgatctc
     1561 cgcttctgaa agcattaccc gcaccgttaa cgatattctc gataacgtga aaacgcgtgg
     1621 cgatgaggcc ctgcgtgaat acagcgcgaa gtttgataaa accacggtta ccgcgctgaa
     1681 ggtgtctgct gatgagatcg ccgccgccag cgaacgcctg agcgacgagc taaaacaggc
     1741 gatggcggtg gcagtaaaga gtattgaaac cttccacact gcgcaaaaac tgccgccggt
     1801 agatgtagaa acgcagctag gcgtgcgttg ccagcaggtc acgcgcccgg tggcttcagt
     1861 gggtttgtat attccgggcg gctccgcccc gctcttctca acggtattaa tgctggcaac
     1921 tccggcgcgt attgcgggct gtaaaaaagt ggtgctgtgc tcaccgccgc cgattgccga
     1981 tgaaattctt tatgcggcgc agctgtgcga tgtgcaggac gtgtttaacg tcggcggcgc
     2041 acaggccatt gccgcgctgg cgtttggtac ggaatctgtg ccgaaagtgg acaaaatctt
     2101 cgggccgggt aacgcctttg tcaccgaagc aaaacgccag gtaagccagc gtctggacgg
     2161 tgcggcgatc gatatgcccg caggcccgtc ggaagtgctg gtgattgctg acagcggcgc
     2221 tacgccggat tttgtggctt ctgatttgct ttctcaggct gaacacggcc ctgactcaca
     2281 ggtaatttta ctgacccccg atgctgatat ggcgcgtcgc gttgccgagg ccgtcgaacg
     2341 ccaactggca gaactgccgc gtgccgaaac cacccgccag gcactgaacg ccagccgcct
     2401 gatcgtgact aaagatttag cgcagtgcgt ggagatctcc aaccagtacg gcccggagca
     2461 cctgatcatt cagacccgca acgcccgcga actggtcgat ggcatcacca gcgccggttc
     2521 ggtatttctt ggtgactggt caccggaatc ggcaggcgac tacgcctccg gcaccaacca
     2581 cgtgctgccg acttacggtt acaccgccac ctgttccagc ctcgggctgg cagatttcca
     2641 gaaacgcatg accgtacagg aactgtcgaa agaaggtttc tccgcgctgg cttcgaccat
     2701 tgaaacactg gccgccgccg agcgcctgac cgcccacaaa aatgccgtta ctttgcgtgt
     2761 taacgtcctt aaggagcaag catgagcacc gtgactatta ctgatttagc gcgtgaaaac
     2821 gtccgcaacc tgacaccgta tcagtcggcg cgtcgtctgg gcggtaacgg cgacgtctgg
     2881 ctgaacgcca acgaatatcc cacagccgtg gagtttaagc ttactcagca aacgctcaac
     2941 cgctacccgg aatgccagcc aaaagcggtg attgaaaatt acgcgcaata tgcaggcgta
     3001 aaaccggaac aggtgctggt cagccgtggc gcggacgaag gtattgaact gctgatccgc
     3061 gctttttgcg aaccgggtaa agacgccatc ctctactgcc cgccaacgta cggcatgtac
     3121 agcgtcagcg ctgaaaccat tggtgtcgaa tgccgcacag tgccgacgct ggaaaactgg
     3181 caactggact tacagggcat ttccgacaag ctggacggcg taaaagtggt ctatgtttgc
     3241 agccccaaca accccaccgg gcaactgatc aatccgcagg attttcgcac cctgctggag
     3301 ctaacccgcg gtaaggcgat tgtggttgcc gatgaagctt atatcgagtt ttgcccgcag
     3361 gcatcgttag ctggctggct ggcggaatat ccgcacctgg cgattttgcg taccctttcg
     3421 aaagccttcg cgttggcggg gcttcgttgc ggatttacgc tggcaaacga agaagtcatc
     3481 aacctgctga tgaaagtgat ccccccctac ccgctctcga cgccggttgc cgacattgcc
     3541 gcacaggcgt taagcccgca gggaatcgtc gccatgagcg aacgggtggc gcaaattatt
     3601 acagaacgcg aatacctgat tgccgcactg aaagagatcc cctgcgtgga gcaggttttc
     3661 gactctgaaa ccaactacat tctggcgcgc tttaaagcct ccagtgcagt gtttaaatct
     3721 ttgtgggatc agggcattat cttacgtgat cagaataaac aaccctcttt aagcggctgc
     3781 ctgcgaatta ccgtcggaac ccgtgaagaa agccagcgcg tcattgacgc cttacgtgcg
     3841 gagcaagttt gatgagtcag aagtatcttt ttatcgatcg cgatggaacc ctgattagcg
     3901 aaccgccgag tgattttcag gtggaccgtt ttgacaaact cgcctttgaa ccgggcgtga
     3961 tcccggaatt gctgaagctg caaaaagcgg gctacaagct ggtgatgatc actaatcagg
     4021 atggtctggg aacacaaagt ttcccgcaag cggattttga tggcccgcac aacctgatga
     4081 tgcagatctt cacctcgcaa ggcgtgcagt ttgatgaagt gctgatttgt ccgcacctgc
     4141 ccgccgatga gtgcgactgc cgtaagccga aagtaaaact ggtggagggt tatctggctg
     4201 agcaagcgat ggatcgtgcc aacagttatg taattggcga tcgcgcgacc gacattcaac
     4261 tggcggaaaa catgggcatt aatggtttac gctacgaccg cgaaaccctg aactggccaa
     4321 tgattggcga gcaactcact aaacgagacc gttacgccca tgtagtgcgc aacaccaaag
     4381 agacgcagat tgacgtccag gtgtggctgg atcgcgaagg tggcagcaag attaacaccg
     4441 gcgttggctt ctttgatcac atgctggatc agatcgccac ccacggcggt ttccgcatgg
     4501 aaatcaatgt caaaggcgac ctctatatcg acgatcacca caccgtcgaa gataccggcc
     4561 tggcgctggg cgaagcgtta aaaattgccc tcggcgacaa acgcggtatt tgccgctttg
     4621 gttttgtgct gccgatggac gaatgccttg cccgctgcgc actggatatc tctggtcgcc
     4681 cgcacctgga atataaagcc gagtttacct accagcgtgt gggcgatctc agcactgaaa
     4741 tgatcgaaca cttcttccgt tcgctctctt acaccatggg cgtgacacta cacctgaaaa
     4801 ccaaaggtaa aaacgatcat caccgtgtag aaagcctgtt caaagccttt ggtcgtaccc
     4861 tgcgccaggc catccgcgtc gaaggcgaca ccctgccctc gtcgaaagga gtgctgtaat
     4921 gaacgtggtg atccttgata ctggctgcgc caacctgaac tctgtgaagt ctgccattgc
     4981 gcgccacggt tatgaaccca aagtcagccg tgacccggac attgtgttgc tggccgataa
     5041 actgttttta cccggcgtcg gcaccgcaca agcagcgatg gatcaggtgc gtgagcgcga
     5101 gctgtttgat ctcatcaaag cctgtaccca accggtgctg ggcatctgct tagggatgca
     5161 actcctggga cgacgcagcg aagagagcaa tggcgtcgac ttgctgggca tcatcgacga
     5221 agacgtgccg aaaatgaccg actttggtct gccactgcca catatgggct ggaaccgcgt
     5281 ttatccgcag gcaggcaacc gcctgtttca ggggattgaa gacggcgcgt acttttactt
     5341 tgttcacagc tacgcaatgc cagtcaatcc gtggaccatc gcccagtgta attacggcga
     5401 accgttcacc gcggcggtac aaaaagataa cttctacggc gtgcagttcc acccggagcg
     5461 ttctggtgcc gctggcgcta agttgctgaa aaacttcctg gagatgtgat gattattccg
     5521 gcattagatt taatcgacgg caccgtggtg cgtctccatc agggcgatta cggcaaacaa
     5581 cgcgattacg gtaacgaccc gctgccgcgc ttacaggatt acgctgcgca aggtgccgaa
     5641 gtgttgcacc tggtggatct gaccggggca aaagatccgg ctaagcgtca aatcccgctg
     5701 attaaaaccc tggtcgcggg cgttaacgtc ccggtgcagg ttggcggcgg tgttcgtagt
     5761 gaagaagacg tggcggcgtt actggaagct ggcgttgcgc gcgtggtggt cggttccacc
     5821 gcagtgaaat caccggacgt agtgaaaggc tggtttgaac gtttcggtgc cgatgcgctg
     5881 gtgctggcac tggatgtccg tattgacgag caaggcaaca agcaagtggc agtcagcggc
     5941 tggcaagaga actcgggcgt ttcactggaa caactggtgg aaacctatct gcccgtcggc
     6001 ctgaaacatg tgttgtgtac cgatatctcg cgcgacggca cgctggcggg atctaacgtc
     6061 tctttatatg aagaagtgtg tgccagatat ccgcaagtgg cgtttcagtc ctccggcggc
     6121 attggcgaca ttaacgatgt tgctgcccta cgtggcactg gcgtgcgcgg cgtaatagtt
     6181 ggtcgtgcat tactggaagg taaattcacc gtgaaggagg ccatcgcatg ctggcaaaac
     6241 gcataatccc gtgtctcgac gttcgtgatg gtcaggtggt gaaaggcgta cagtttcgca
     6301 accatgaaat cattggcgat atcgtgccac tggcaaaacg ctacgccgaa gaaggtgcag
     6361 atgaactggt gttctacgat atcaccgctt ccagcgatgg ccgtgtggta gataaaagct
     6421 gggtatctcg cgtggcggaa gtgatcgaca ttccgttttg tgtggcgggt gggattaagt
     6481 ctctggaaga tgccgcgaaa attctttcct ttggcgcgga taaaatttcc atcaactccc
     6541 ctgcgctggc agacccaaca ttaattactc gcctggccga tcgctttggc gtgcagtgta
     6601 ttgtggttgg tattgatacc tggtacgacg ccgaaaccgg taaatatcat gtaaatcaat
     6661 ataccggcga tgaaagccgc acccgcgtca ctcaatggga aacgctcgac tgggtagagg
     6721 aggtgcaaaa acgcggcgcc ggagaaatcg tcctcaacat gatgaatcag gacggcgtgt
     6781 gtaacggtta cgacctcaag cagctgaaaa aagtgcgtga agtttgccac gtcccgctga
     6841 ttgcctccgg tggcgcgggc accatggaac acttcctgga agccttccgc gatgccgacg
     6901 ttgacggtgc gctggcagct tccgtattcc acaaacaaat aatcaatatt ggcgaattaa
     6961 aagcgtacct ggcaacacag ggcgtggaga tcaggatatg ttaacagaac aacaacgtcg
     7021 cgaactggac tgggaaaaaa ccgacggtct gatgccggtg attgtgcaac acgcggtatc
     7081 tggcgaagtg ttaatgctgg gctacatgaa cccggaagcc ttagacaaaa ccatcgaaag
     7141 cggtaaagtc accttcttct cgcgcactaa acagcgactg tggatcaaag gcgaaacctc
     7201 gggcaatttt ctcaatgtgg taagtattgc cccggactgc gacaacgaca cattactggt
     7261 gctggcgaat cccatcgggc caacctgcca caaaggcacc agtagctgct tcggcaacac
     7321 cgctcaccag tggctgttcc tgtatcaact ggaacaactg ctcgccgaac gtaaatatgc
     7381 cgacccggaa acctcctaca ccgccaaact gtatgccagc ggcactaaac gcattgcgca
     7441 gaaagtgggc gaagaaggcg tggaaaccgc actggcagca acggtacatg atcgctttga
     7501 gctgaccaac gaggcatctg acttgatgta tcacctgctg gtgctgttgc aggatcagga
     7561 tctggattta acgacagtaa ttgagaacct gcataaacgg catcagtgag ttacgggctg
     7621 agcaggtgtg atgttgttgt cacacccgga gccattgccc gatgcgcaaa cttatcgtgc
     7681 ctacacatta tctcaacaca cgtaggccgg ataagatgcg ccagcatcgc atccggcaaa
     7741 aaaaacgggc atggtgtcac caccctgccc gtttctctta aatgcacaat aatattactt
     7801 cgcgttgtaa ttacgcagag cgttacgccc caacacaatc cccgcgccaa ccatgccgcc
     7861 cagaagcact gccagaatca aggtgattgc ctttttcgga ctatcgcgac gaataggtaa
     7921 cgtcggtttc atcacatagc ggtaagcatg aatatcaaga tcatcaaact ttaatttttc
     7981 aatatccaac aggttttgac gtgtctgata gtagtttggt gagaacacca acggacgagt
     8041 cgcttcatgc ttaatcatcg actccagcgc ttcgctccct agaaggaaca acgtatcttg
     8101 cgtcacatct tcagtctgct gaacctgtgg ctttgtcacc tgcgcctgat tcgcatactg
     8161 caacgcttcc tgaatctgac ggatacgcag atctttctgc tcctgcgcga ccacttcctg
     8221 ggttcttaaa gagtcctgca agtttttccg tcccagagca atgttgtcct tgagatcctt
     8281 ttctagctct tgattcactt tatcatcaac ttgctgaatg tattgggcca acttcatttg
     8341 tgcgccctct gcagtttgcc caacataaga aacagtcaat ggtaattgct ggttcttaac
     8401 agaaggttcg atggtaagtt tttctggctc ttcctgatta tccagcgttt ctgctaatgc
     8461 agagaaggca gaactgaagc gaccaattaa cgtctcctgc aaatccgata ctttcggtgc
     8521 agcctgacca tagataacat tcatggcatt gttatagcca gcaatttgcc ccacgtcggg
     8581 ctgagtgata attgctgttg acgtccattt ctccttcgct actgccaaat aaccaatagc
     8641 cagggcaata gccacaatga cggaaatgat aattgtcatc ttgccacgcc acaactgcac
     8701 tagtaaatca atcaaatcaa tctgttccgg gtcatggttt tgcccagaaa cattattatt
     8761 ttctactctc atcattaccc taactgacgg aaatttccaa gtccgcatag tttagcgata
     8821 atccccgtga atgctatagg ataagtgata aaaataatga attattgcgg aggtaacaaa
     8881 agacaatcag ggcgtaaata gccctgataa caagatgtta gtcgctgcca aagagatcgc
     8941 gggtgtagac cttatctgcc acatccttaa gctcttctgc catacggttg gagataatga
     9001 cgtcggcttg ttgtttgaag gtggcgagat cacgttccag gcgagagttg aagaatgagt
     9061 cttctttcat caccggctcg tagatgatca cttcaacgcc tttcgccttg atacgcttca
     9121 taatcccctg aatagaagac gcacggaagt tatctgaccc gctcttcata atcagacgat
     9181 aaatacccac cacttgcggt ttgcatcgac aattgccgag atcaggttat tcggcacaga
     9241 ctggtagttc gccagtaact gcttggtatc tttcggcaga caataaccac cataaccaaa
     9301 cgacggattg ttgtaatggt tgccaatacg cggatcgaga caaacgcctt cgataatctg
     9361 gcgagtattc agacctaaac tttctgcata actatccagt tcattaaagt atgctacgcg
     9421 catcgccaga taggtattag cgaaaagttt aatcgcttct gcttcagtgg agtcggtaaa
     9481 cagggtcggg atattttgct taatcgcgcc ttcctgtaac aacgcagcaa aacgttctgc
     9541 gcgttctgaa cgctcaccga tgacaatacg tgacggatgg agattatcgt aaagggcttt
     9601 accttcgcgg aggaattccg gagagaaaat aatattttca gtacgatatt tcttatgcat
     9661 cgctgcggta aaaccaacgg gaaccgttga tttgatgacc ataaccgcat aaggatttat
     9721 ctcaactacg tctttaatta ctgattctac actggatgta ttgaaataat tagttttagg
     9781 atcatagtcg gttggagtgg cgatgatgac ataatcagca tcccggtagg cttcattttt
     9841 atctaatgtg gcattaaagt gtattttatc tgattgcaaa aactgctgaa tttccttatc
     9901 aacaatagga gatatccgat cattcagcat agcaacgcgt gacggtaaaa tatctaatgc
     9961 cacaacctca tgattttgtg cgattagaag cccgtttgac aagcctacat agccagtacc
    10021 ggaaatggtg attttcattt catgctctca gaattaactt aactgtgaat catgatgttt
    10081 ttagcatcgt gataagatgc taaaagtttt aacgctacgt tgtcagatgg tcaacgcaac
    10141 gaaaaacaat tcagattttc tttataagaa tagctgatgt ttattaagtt aatattaagc
    10201 agtgaaattt agtctataag atatttggca aaaaaaagcc cggtgcaata tacgccgggc
    10261 ctcaatttta ttgttggtta aatcagatta atccagccat tcagtatgga acacaccttc
    10321 tttatcaatg cgcttataag tatgcgcccc gaaatagtca cgctgtgcct ggatcaggtt
    10381 cgcaggcaga accgcggcac ggtagctatc gtaataggca accgcagcgg cgaaggtcgg
    10441 aaccgggata ccgttctgta ctgcataagc gacgacatcg cgcagcgcct gctggtagtc
    10501 atcggcaatt tgcttgaagt acggggccag cagcaggtta gcgatctgcg gattttcggc
    10561 ataagcatcg gtgattttct gcaggaactg cgcacggatg atgcagccag cacggaaaat
    10621 cttcgcgatt tcgccgtagt tcagatccca attgtattct tcagacgcag cacgcagctg
    10681 agagaagccc tgagcgtaag aaacgatttt gcccagatac agcgcactgc gaactttttc
    10741 gatgaactca gccttgtcgc ctgctgactg agcttgcgga ccagagagaa ctttagatgc
    10801 ggcaacgcgc tgatctttca gagaagagat ataacgtgca aacacagact cggtaatcag
    10861 cgacagcggt tcgccgagat ccagcgcgct ctggctggtc catttaccgg tgcctttgtt
    10921 tgctgcttca tccagaatca catcaaccag gtagttaccg tcttcatctt ttttggtgaa
    10981 gatatcttta gtgatgtcga tcaggtagct gctaagttca ccgttattcc actcggtgaa
    11041 agtctgggcc agttcttcat tagatagatt caggccacct ttgagcagag aatacgcttc
    11101 agcaatgagc tgcatatctc cgtattcaat accattgtga accatcttca cataatggcc
    11161 tgcgccgtcg gcaccaatat aagtaacgca tggctcgccg tcttctgcca cggcagcgat
    11221 tttagtcagg ataggcgcaa ccagctcata agcctctttc tgcccgccag gcataataga
    11281 aggacctttc agcgcgccct cttcaccgcc ggaaacaccg gtaccgatga agttaaagcc
    11341 ctctgctgaa agctcacgat tacgacgaat ggtgtcctgg aagaaggtat taccaccatc
    11401 aatgatgatg tcacctttat cgagatatgg tttcagggaa tcaatcgcag catccgtgcc
    11461 tgcacctgct ttcaccatta acaggatgcg acgaggcgtt tccagagatt caacaaactc
    11521 tttcaccgta tagtaaggag ccagtttctt gcctggattt tcagcaatca cttcttccgt
    11581 cttttcgcgg gagcggttga aaatagagac ggtataacca cgactttcga tgttgagcgc
    11641 aaggttgcgc cccatcactg ccataccgac tacgccgatc tgttgctttg acattgttta
    11701 ctcctgtctt ctatattatt aaccgtctat taaccgttat attattaacc gtcgtgattt
    11761 tcatcttcta atcaaggaat acatatggtt acagcatgct tcttacttta tagcgataag
    11821 tatgccattg ggtgtatcca taagaacttt gaattttgga tacttttctg gattaaaatt
    11881 agcatagctt cgtttagata taacaataaa cgaactatta aaagatttca tcttctctat
    11941 taaatcatcc tgtgggatta cctcacactc gggattacta atagtccaat cactatatct
    12001 tgctaatcca aagacgtcac caattgccat ccctttatag aagtaaatac catcctccaa
    12061 gcctaattgg ataagattat ctccatataa ctcactatat tcacttgctt tcttcatcag
    12121 ttcatagcta tagcgacttt ctaagcgtga attaaaattc aagtattcag aataaaaata
    12181 tttgaattca aaatacaacg atcgccccga aaaaaaataa tacacacaag gataaaatat
    12241 cgtcccaact tgtaatttgt aatgttgata tcttttatta aacttgcaat tgagatcgca
    12301 acctgcaata ctgacaatgg gtaaatgggt gataaatacc tgtcaccttg cgtaacagaa
    12361 aaccaaaata agacatatgt taaaacaatc gagaaaaata cccaaagtat tttattattc
    12421 ctaagtgtaa agacagacag tatagcataa aaaacaacat cactgccaac tttattaatt
    12481 gcttgttgca aatctaatgt ggcaggactt ataccatgtc gagcttgttc tgctgtttga
    12541 acttgtagat caatttcatt ccataggtag tgaccaaata tatttcctcc agcaggacta
    12601 aaaggatcac cagagatcca aaaactacgg acataccaat aaatgccacc aagaattgtt
    12661 gccaagctaa aaataagaaa tgtctgtatg tttctctttt tcgctatcaa tataacaaat
    12721 atgatggctc catatatggc accaaatagt tttacgccac aagcaattcc aataaaaaaa
    12781 ccacataaaa aaaattaatt cattttgtgt tttattgttt tctgagcgaa tatatataag
    12841 aatactggct gcagcaaaac aaagatagcg ttaaatcaat atatgcataa ccaagatgat
    12901 ctttaagtgg attgatagta aatagtagga ttgtagcaat aatgcctgga taataagttt
    12961 tcgtaatttc ccatgaacag ccaacaagtc cacaggctat tacaaaaaaa cagatatttg
    13021 ctaaaaactg agctaacctt acatctccta actgtaaacc taaagccatt aaaagatctg
    13081 cattttgcgg gaacagagga aaacgcaaat attgttcaac aacaattgca tggtgctcaa
    13141 ctaatgaacg agccaaaggt aagtgataca tagtatcatc ccaatcaccg ggagctcgca
    13201 gggacgataa caatgtaacc acaaaaatca aaacaagaaa taaaataaca aacagcgtac
    13261 tgaactcaat attatttatt gccagccatc taaattgctg acgtttaata atgcttaaaa
    13321 cccaacacac cgtaaaaatt aaaaagatag caatcttatt aaattgcccg accatgccca
    13381 gaaagtaaat tatcaaaata tttaaagaca tccctatgca taggcaataa aaaataaaga
    13441 aaatttgtta agtcctgcca ccccctcctt aaaaaaagat gacaatacaa tattaccaat
    13501 gacaaaaaat gtcattccaa aagcaaaaca atacacaaca tacaaataca acggcaattc
    13561 aatcatagta aaatactcta tttacattat cagtttatag tttttttcag aaagataaaa
    13621 taatgccttc atttcacgtc tagttgttgt atttgaatgc aaaataatgc cacatacaaa
    13681 tgccattatt gacgataaca tgatggaggc agttaaaaag gcagtcggaa atcttctcac
    13741 caaacctgta tcaatataat caacaatgat gggcaatcct aaaattatag acaaagcagc
    13801 aagtaaaacg ccgataatac taaaaaagta tagtggccgt tcaagcgagt aaagtttaat
    13861 tattgttttc agaatcttga acccatcact ccaagttgat agtttgctca cactcccttc
    13921 agatcgctcg ccatattttg tattaacctc accatacttc atacgtaatt ctaaagcatg
    13981 aattgtaagc tcagtttcga tttcaaatcc ccttgataga catggaaaag atttaacata
    14041 gcgtctggaa aatacacgat accccgacaa catatcagta aaattccctt taaagatagt
    14101 ctgaacagta ccagtcaaca atttatttcc tcagcgatgc ccttttctgt atgtattttg
    14161 atctccattc tcttgacgac atcctacaac catatccaat tgatttttta tgagatggtt
    14221 aatcatttct ggagcagaac ttgcatcata agtgtcatct ccatcaacca ttagataaat
    14281 atccgcatct acatctgaga acatacgtct tacgacattc cccttgcctt tcagaggaac
    14341 agagtgaact gttgctccag catcctctgc cacttgtgct gtactgtcag tcgaattatt
    14401 atcaaataca tatatagaag cagttggaat attcgttttg aaatcattta tgacttttgc
    14461 tattgccttc tgctcattat aacaaggaat aattacagcg atattaaaag agtgatacat
    14521 aagcccctca tataaactat ttagctaatg tgcaagtaat tgtgttttta acaaatcgtt
    14581 tactaacgaa aaatgaaata ttttgatgcg caatatccta aaaccaaact aaaaagagaa
    14641 aaggtgaaca atgtaaatat tggagataaa gctaagagat caaataaaac gccgaataaa
    14701 taactcatcg cccccataaa aaatataaat ataaaatatc tagccttggt tgcagatact
    14761 ttaaatgagc atcttgcatt tgcaaaaaaa gaaaaggttg ctgcacatat aaaaccaaca
    14821 atgttacttc ggccttgacc aaaagctaaa ttattataac acgcataaaa taccccccaa
    14881 tgtaaagcag tattgacaat tccaattaaa aaataactaa aaaaagaact ggtcaataac
    14941 ttccctattt ttaacatcct ttattttgct ccagaagtga ggttattact aatttggata
    15001 ttttctatag aaaataccca taaaactaca acgcctataa taaaaattcc catgggtgta
    15061 aaaagaaatg gattagttat agaaacggta atactgaaaa aaagtattaa cgacatcatt
    15121 aatgatgttt ttgtactatt taaaaaatag cctttcaaaa agataagcaa cataggaact
    15181 aatgatataa atacaccaat aacgcctgtt ttctgaagta tctcaagagg tactatttca
    15241 atccttaatc gatctaatat aaatgaacca aatcctcttc caaacaagaa cgtcgctaaa
    15301 tcaacatttt tataataaaa atataaatca ttaaatctaa cagagtcaga atcttcactg
    15361 cgtgatccga ccatgtaaag cataattata aatagaacgc tgcccaatat tattataata
    15421 gtatttaaat atagcttatg taatttaaaa ctggctaata acagtgataa tgttgtaaaa
    15481 agtaatacac ctcttgattc tgtcaataat atcgcaagca ctattatgag acattttaaa
    15541 tattttttgt cgacaaaact gataatcgca ccgacaccaa aaaagtagaa ggatttggaa
    15601 aaaaaagccc catcgggacg aaaaaagaac tcgctagtca atgataagtg ttcataaatt
    15661 aaattaaaat ttaacaaacc gaaattcaac aaaattatgt atgaaatata taacagtgat
    15721 ccatataatg caaatgcatt aactatttta ataaaaatct cacatgaaaa tacttttaaa
    15781 gtatatctta gtgtaaatag atatataagt gacagcataa aaaggtatgg ttgcacatca
    15841 aacagtatat catttatttt attaccagct aataagccga caaagaagcc ataaaaagga
    15901 aataaaagca accatataaa aagactgaca catttttggg tgattgaaaa attgaatgta
    15961 actaaattaa aaattaaaag tataaaacta aatacaaata ataaatatcg caaagggaat
    16021 attcccaatg gctccagtaa tcttccacca ccaccaataa tcagttcaat acataaaaat
    16081 gttataaaaa ttttatttat attattcata ttcgtaaggt gatgtttttt aaaatatgtt
    16141 taaaatcaac attaataaaa tataaatttt atcagccttt ttccgcctta acttccaaaa
    16201 acttaaagcc aagatcaaat tgcctatata acctttaccc aattgattat tcacaaagct
    16261 atcatcagct aaggcattag ctattttagt tacttctttt cgataccact tgtttctcac
    16321 caaaccaagc cgtttgtatt ttgctttaaa actaatgttt gctcccactt ggttgttttc
    16381 atgctgtctg tacagcattg ttggttttct atcaatatac cacgaataat ttctcgtccg
    16441 cgcaaatgaa tataaaaacc aatcatgcag acagatatct tgtgcagcat ttttattatt
    16501 tatgatgaac ttcttaaatt caataagcgt ttcttttttt ataacatatg tacaccccgg
    16561 acctgctgct tcaaaaaaat aatcgaattg agtttgcgac tgagccttat caactaaatt
    16621 ttttcgccct gatggataat atgcaattac attgctggaa taacaattcg cattattaaa
    16681 taccaagcag tcaattgcat tttttatttt atctttatac caaatatcgt cctgatcact
    16741 aaaacagata taatcatagt tttcaagatc gacatcttta attaatctat aaaaattttt
    16801 acctgccccc ccaaacttat tgacgcttgg aagtatattt ataattgagg gatatgataa
    16861 ttgaaattct tcacaaatat ttaatgtatt atcagttgat aaatcatctg atataaaaat
    16921 tgagatgtct acatcttttt gttcaataat actttttaat tgttcctcaa tccagcattc
    16981 gccattatac gtagccataa gtacagctac tcttttttga tcagaactat gcattataac
    17041 gaccgccccc agtaattcct cttattccat ccagcgtagc caaaatatgt tttttcatat
    17101 attcaatttt atttttttga gttgcgataa ttaaagtcgt atgaattaaa gaaaaaacaa
    17161 cttccctaat agagagtaaa agaggcacat gattttttct aagcaaaaga aatgcatttc
    17221 ttactcgata ataacgacgt actggcgagt gaattggtat gttaatccca cagattttta
    17281 ctgacttatc cccaatagca tgaaccattt ctatatcagg aatgatatga actaatatgc
    17341 catagtttaa gcatctaaga caccattctg tatcgacata atcaataaaa agttcctcat
    17401 ccatcattcc aacaatctca aaaacttctt ttgaaaccat ggttccggat gagattgtta
    17461 ctgaactttt aaacggtgtc tgtccttcag tgacatgaat tttctctcga agaccatttt
    17521 ttttgatatt acaaatagga taataatgtg acttatctct atcaataaaa actgggccag
    17581 ctatttttgc attttcatta tccatatagc aggaaagttt tttgataaac tcattgcgaa
    17641 tacggctatc ttgatcaaaa aaaattatct tctctgcccc tttttcaaga gaatatcgaa
    17701 tcccaatatt ttgtgctttt gctatacctt cattcctacc aagacaaatt aaatgaactt
    17761 tttcaatgtc tgctaattta tctttcaagg tgaaattagt cctattatcg actactacaa
    17821 cgtaacaatt ttgctccttt agttcagtga tcagtgcatt cagattttta agttcgggat
    17881 tatatgtcac aatgacagcg taaatattac tattcattat ccaagtgact cagtaattgg
    17941 ttaattttag ttttcatata cagaacacat tctagtataa agccattgta ataacagagc
    18001 atctaaaatt acacgtatgc tccaggatat tgcggcaccc agtaacccat atttttcgac
    18061 cataaaatac aaaagggcta aataaggaac caattcagca caatgaatca aagcagtaat
    18121 tttagatttt cctaatgcct ggattgcaga gaatggtatc attgccagcg cattgaagaa
    18181 aaaaccgatc aataacacat ttaatatatt aaaggaatta atttgataat tctcaccaaa
    18241 ccatattttc aatacaagcc ctgaatataa caagcctatg attatcaccg gcaaacagat
    18301 tagaaacatt aaaagcaatg atttatttac attacgttta aattctttaa agtctttgat
    18361 gttacttaac cttggaaaca ctgccctccc gattgctgca ggtattattc ctaattttag
    18421 aataacctcc gctggtgcag aataaaatgc aactttatct gcccccataa tatttgacac
    18481 tataaatcga tcaaaataca ccatgaccgg acttatgata ttacttactg tcatccaacc
    18541 gccaaagaaa aagagtcgtt taaaggtttt ataacaaaaa tgaacccccg aaattttaat
    18601 atcattcctg acataatagg cagagactaa aatcgaaata actcttgcaa atattaaacc
    18661 agcaaccgct gccgacaatg tagcactata aaaaacaaat atggccggaa ttcccgcgat
    18721 gcaagagctt gatatagatt tttgaatatt tacaatgcca aatttttcat ccccctcaag
    18781 aatggctgac cataattgat ttagaataaa taatggaatg caaatagcca acagtttaaa
    18841 tgctagttgt atatcactat gttcaacacc agaaattttt agataattaa caattccatc
    18901 agagaaaatc aataataaaa aagcaccaaa gcatgaaaat agcaccaaaa aagatgtact
    18961 tgttgaaatt accttggttc tttcatgatg attatcacga tgaattgcaa tttctctaat
    19021 aacagagcga gtcaggccta catcaaaaat tccagcatac ccaacaagtg caattgcaat
    19081 tgtataaaca ccgaaacctt caggccccaa acctcgagcg agaaatccta gcgaggggat
    19141 agcaattaaa gttggtattg catagccaaa aaggttccag acactatttt ttattatgct
    19201 cattattaca tttctttatc atataaaata atcagcatct acaaattttt gaccattaat
    19261 atctttatca gaaagcaatg gattttgaac cggccattct atatttatac ttttgtcgct
    19321 ccataaaatc ccacgctctt gcatttttga atagaaatta tttgttttgt agacaaattc
    19381 tgcttcatca ctaagaacgc aaaacccatg agcgaatcct tccggtatcc ataattgacg
    19441 cttattctcc gccgacaaat ttacaccaac ccacttgcca aatgttggtg atgatttccg
    19501 aatatcaaca gctacgtcaa aaacttcacc gacaacacag cgtaccagtt ttccttgagc
    19561 ataaggttcc agctgatagt gcagtccgcg taacacaccc ttagttgatt tagaatggtt
    19621 atcctgaaca aactcaacct ttcgtcctac agcctcttcg aaaactttct gattgaagct
    19681 ttccataaaa aagccccgct catcaccaaa tacttttggt tcaaaaatta gcacatcagg
    19741 aatttcagtt ttaattacgt tcattttatt aataaccttt aatcattttc agcagatact
    19801 gaccataagc atttttcttc agcggctcgg ctaatacctt cacttgctca gcatcaataa
    19861 agcctttacg gtgagcaatt tcttccgggc aggaaacttt cagcccctga cgctcttcaa
    19921 ttgttgcaat gaagttgctt gcttcaataa gactttgatg tgtccccgtg tccagccatg
    19981 cataaccacg ccccatcata gcgacagaca aacgtccttg gtccatataa atacggttaa
    20041 tatcggtaat ttccagttcg ccacgtgcag atggtttaag gtttttcgcc atttctacaa
    20101 cgtcattgtc atagaaataa agcccagtaa ccgcatagtt actttttggt tcctgcggtt
    20161 tttcttccag actaattgcc gtaccgttat catcaaattc caccacaccg tagcgctcag
    20221 gatcatttac gtgataggca aaaaccgttg cgccactctc tctgttaaca gcggtatcca
    20281 ttaatttcgg caggtcgtga ccatagaaga tattatcacc aagtaccagc gcacaatcat
    20341 caccacctat aaattcttca ccaatgataa atgcttgcgc cagaccatcc ggactcggtt
    20401 gtactttata ttgcagatta agcccccact ggcttccatc accaagcaat tgttgaaaac
    20461 gtggtgtatc ctgcggcgta ctgataatca gaatatctcg aatacccgcc aacataagcg
    20521 ttgaaagcgg ataataaatc atcggtttat cataaatcgg tagcagttgt ttgctcacag
    20581 ccattgtcac aggataaaga cgagtgccgg aaccaccagc cagaataata cctttacgcg
    20641 ttttcatttc accattcctt ttaattcatc tcgctctagc atcatgagcg agatgcaata
    20701 atttgttaaa ttgccgtggt cgtaaataat tcgttgagca tacgtttcac gcccacctgc
    20761 cagtcaggca agacaagcgc aaagttctgc tgaaattttt cggtattgag gcgagaatta
    20821 tggggacgac gtgctggtgt gggataggcc gttgtcggca cggcattaag tttgttaaga
    20881 gcaaggttga tccccgctct gcgcgcttct tcaaatacca gagcggcata atcgtgccag
    20941 gttgttgtac caccagcaac cagatggtac aaaccagcaa cttctggttt atttgcggcc
    21001 acgcgaatgg catgagcggt gcaatcagcc agtaattcag cccccgttgg tgcgccgaat
    21061 tgatcgttta ttacagccaa ctcttcgcgt tcttttgcca ggcgcaacat cgttttggca
    21121 aagttatttc ctttaccagc gtatacccag ctggtacgga aaataagatg ctttgcgcaa
    21181 tgttcttgta acgctttttc cccagctagc ttggtttcac cgtagacatt tagcggtgct
    21241 gttgcatccg tttccagcca tggcgtgtca ccgttgcctg ggaaaacata atcagtggag
    21301 tagtgtataa cccaggctcc gacttcatta gctgcttttg caatcgcttc gacgcatgtc
    21361 gcgttaagta attgcgcgaa attcggttct gattccgctt tgtctactgc agtgtgagca
    21421 gcagcattaa caataacatc ggggcgaatt tttttgacgg tttcagccac accttcgggg
    21481 ttgctgaaat ctccacaata atcagtggag tgaacatcga gcgcaatcaa gttacccaga
    21541 ggagccagag cacgctgcag ttcccaaccc acctgccctg ttttgccgaa aagcaggata
    21601 ttcattactg acggccctca tagttttgtt caatccacga ctgataagta ccgcttttca
    21661 cattctcaac ccaatttgta ttagccagat accattccac cgttttacga atcccgctct
    21721 caaacgtttc ttgcggtttc cagcccaatt cgcggctaat tttatcggca tcaatggcgt
    21781 aacggcggtc atgccccggg cgatcggcaa cataagtaat ttgctcacga taagatttct
    21841 ctttcggtac aatctcatcc aacaaatcac aaatagtgag cactacgtcg atgtttttct
    21901 tttcgttatg tccaccaatg ttataagttt cacccgcttt accttcggtt acgacggtat
    21961 ataacgcacg agcatgatcc tctacataca accagtcgcg aatttgatcc cctttaccat
    22021 aaataggtaa tgccttacct tccagagcat tcagaataac caatggaata agtttttccg
    22081 ggaagtgata aggaccataa ttgttcgagc aattagtcac aatggttggt aaaccataag
    22141 tccgcttcca ggcacgtacc aaatgatcgc tggaagcttt agacgcagaa tacgggctac
    22201 ttggcgcgta agctgtcgtt tccgtaaata gcggtaacgc ttcgttatta tttgcttcat
    22261 ccggatgggg taagtcacca taaacttcat cagtagaaat atgatgaaag cggaaacttt
    22321 ttttcttttc atcattcaga gcagaccagt aattgcgcgc cgcttctaaa agaacataag
    22381 tccccacaat attggtttca ataaatgccg cagggcccgt tattgaacgg tcaacgtggc
    22441 tctctgccgc caggtgcatc accgcgtctg gctggtgctg tgcgaaaata cgcgacatgg
    22501 ctacagcatc gcagatatct gcatgctcaa aagcataacg ttcagaatca gaaacatcag
    22561 cgagtgattc caggttcccg gcgtatgtta atttatcgac attaacaaca ctatcttgcg
    22621 tattatttat tatgtgacga acaacagcag aaccaataaa ccctgcgcca ccagtaacaa
    22681 gtatcttcac ttttttattc catatagcta tagagcatgc tgtgaaatag actgctccag
    22741 atttgattaa tagaggtatt aatacacgct accgcccctg gcttaacagc taccagtgca
    22801 ctgcatgcat ctcaacgatg tggcgagcgt tagccactcg cgccaaatcc gaaaaattca
    22861 aacgttaatt gtcttaccaa acagccgtag aaacaaggaa aatcctggaa aaatttgaat
    22921 aatatgagct atgtaacttc ttgtttttat aggctttata aaaacggcaa cggcaggaaa
    22981 attcaatgag caaaacttat gccacgaatc ttcactgccg ttataatttt cttatcaacc
    23041 gttacatccg gtcagatttt cattattcgc ttaacagctt ctcaatacct ttacggaact
    23101 tcgccccttc tttcaggttg cgtaggccat acttcacaaa cgcctgcata tagcccattt
    23161 ttttgccgca gtcgtaactg tcgccggtca tcagcattgc atcaacggat tgttttttcg
    23221 ccagctcggc aatagcatca gtcagctgaa tacgtcccca tgcaccaggc tgagtacgtt
    23281 ccagttccgg ccaaatatcg gcagaaagca catagcgacc tacggccatg atgtctgagt
    23341 ccagcgtctg cggctgatcc ggtttttcga taaattcaac aatgcggctg actttaccct
    23401 cacggtccag cggctcttta gtctggatga cggagtattc agagaggtca cccggcatac
    23461 gttttgccag cacctggctg cggcccgttt cgttgaaacg tgcaatcatg gcagcaaggt
    23521 tgtaacgtag cgggtcggcg ctggcatcgt cgatcacaac gtctggcagt accacgacaa
    23581 atgggttgtc accaatggca ggtcgcgcac acaaaatgga gtggcctaaa cctaaaggtt
    23641 cgccctgacg cacgttcata atgatcacgc ccggcggaca gatggactgt acttccgcca
    23701 gcagttgacg cttcacgcgc tgctcaagga gtgattctaa ctcataagag gtgtcgaagt
    23761 ggttttcgac cgcgttcttg gacgcgtgag ttaccaggag gatttctttg atccctgcag
    23821 ccacaatctc gtcaacaatg tactgaatca ttggcttgtc gacgattggt agcatctctt
    23881 tgggtatcgc cttagtggca ggcaacatat gcatcccgag acccgctaca ggaataactg
    23941 cttttaaatt cgtcattatt tcatccacct gtaaaatggt tgctgaatta tagcttgttc
    24001 gatttttttc gccagcatca attaccctga attgattact gaattacttg tgatgttacg
    24061 ccgcttcgtt gtggattgca gtagcattgt tcctaagtat ggctccattt ttccaggaat
    24121 ggtcgcaaat ctactccctc cgttccggca atctaaagtt aatcttttcc acattaacaa
    24181 tatggtgatt aatcctgtcg atatcgacgg agctttgccc tctttcattc accgcatgaa
    24241 catttgcaag agacagcagt gtttcttttt tcgccataaa gacgccgcga acgtctttgc
    24301 gcatgtcgaa gttcatgctc aatgctggtc caactgagga ttcctgcatc acattgatat
    24361 ttcgcataaa aagatgttgc ggcttgttgt gtaactccag cgaggcacgc ttcatctcaa
    24421 tgttagttag cgacacaaag gagacggcat tcccggcgga gatttggatg ccgcgcaatt
    24481 tataagcaag atgggtatta tccagttgaa tattattcac tcggaaattt tgcggtatcg
    24541 agagatattt gcctttaatt accccatagc cgattaacat cccggcgcta ttaatcattt
    24601 caatattatc aatcacgaaa ttgtcacaac cgtaaatagc gactgtcgcg ttatcaatgc
    24661 ccgctttctt actgaaatcc ggcgtgatat tgcgggcatt gatattacga ataacaaaat
    24721 gtttaccatt ttcaacatgg atcaactgcc gacaatccga tcccgtgata ttcgccacga
    24781 caaagttttt cactgcctgg tcttccgggt agttgttatc ataagtgctt cccgcaaggc
    24841 ctatgccgat gccccagttg attttgccgt tggtacagtt gatacgctcg atgacatggt
    24901 cagatatcaa aatatcactg tcgttaattg ccacgttcca ttcaatggcg tcgccctgta
    24961 agtcgctgaa cttacagttg gtaatgttgg caccgataat ctggttatga aatccctggc
    25021 gtaagatggc gtaattagcg tggctgacag tgaggttatc gatggtcagg ttgcgcatga
    25081 cccgtttgtt tttgccgcca atataaatct gcattaccgg gccaaagccg ctcatcgcca
    25141 gccctttgat ggtgcagtca gaaccacgca catccagggt aatgttatgc ataccgccgc
    25201 cctctccccc tgtcacctgg ctgccgtcct gtaagacaaa tcgccctctg ccgttgccgc
    25261 gcaggcttcc aaggatgtgt aacgttttgc cgggagggat gaagatgccg gtgttgatat
    25321 tgtcacaaac gaatccggca ggcacgacga ccgtttgccc ttcgctgaag gcttgtttaa
    25381 atgaggcgat ccagtcatgt gggttgtagt cgttaatgtt aacgctttgt cgggcgggaa
    25441 gtgcgcgagc gaaaggggta tggaggaagg caagcgccga gcttgccgtc aggaaggtgc
    25501 gtcgggagag tgttttaaat ggcatacgtt ctcctctata aagcctgcag caagctggcg
    25561 agttctcgat taatcacctg ctggttaaaa tcgtgttcga ctttttcgca cgcacgtttg
    25621 acgaccggag ccagttcgtc ggtatccagt tggctaaacg ccgccagacg ttgtgccagt
    25681 gcgcgagcat cgttctcagg cagcagccag ccggatttat cggcctccac cagttcagga
    25741 attccgctat gcagagtaga aaccaccgga ataccgaccg ccatcgcttc cattagcgcc
    25801 accggaatgc cttccatatc gccatccgcg cccgttaccg atggcaacag gaagacatcc
    25861 gcgtcgtcga gcatctcctt cacttcatgg ctcggtttaa agcccggcat ctctaccaca
    25921 tcttccagtt gatattgttc gatgatggtg cgcaggcgtc gttcccacag gccaatgccg
    25981 aggatgcgat agcgaaatgc cacgccctgc tctttcaact gacggcaggc ttcaatcgcc
    26041 acatgcaggc cttttttctc ggttaagcgt gcgacagata tgatttccag cggcgttgcg
    26101 ggcgctttca ccggacgcgg gctaaagcgc gtcatgtcca cgcccatacg cgatacggcg
    26161 attttttctc tggggcagcc cattttttgc agccttccgg cccacagatc gcttatcggt
    26221 aacatcaggt cgccacggcg aaacagttgt tgatattcgg gagtgtagtg gttgagcact
    26281 tcccgactgg agatatcaat accgtggaag atggtggcaa ttttgccgcg aatgataccc
    26341 agttcgcgta gttttgctgc ggttaccccc gccgggccaa aatgagcgat gaacacatcg
    26401 gcgcgaaacg gtgttgcgac ctgaccacaa atggcagaca aaatcaggtt ccgcgactcg
    26461 gcaccatagc gtttgaggtt gagcgcatgc caggtatttt tacgatgaat gccacgtaag
    26521 gtctggctgg cgcggtggcg cagtttcgct actttgcccg caggttcgtc ctgtaaccag
    26581 cgggttctgg cgacaaggtt gtatttcgtc catgccgcgt gggtgttctc tgtatcgcct
    26641 ttttgcaaag cgacaatctc cacttcaaag cccatatcaa taaacgcggt aatttgattg
    26701 aggacgaagg tttccgacga cagcgggaat ttcaataaga agaagccgac cttcatttca
    26761 cctccccgat gcgctcaagc acagactgca ccatctgcat tcccgtctga cgttcgcgac
    26821 tgacggcttc gttaaatcga gcgttcagca ccggaagctg gcctaaggta tccgcaacca
    26881 tcgcttgcag gctgccgtcc agtaaatgac gaatatcaat cgccatctct ggcagtccca
    26941 gctgctgcat aatcccggcg gatttatgtt catagttgat ggcaatcgcc ggagtagcaa
    27001 agttcattga gataatggcg gagtgcaggc gcgtaccgac ggtgagttca caggctccga
    27061 gaattttgcc catttccaga tcgttgagtt catccatcac tacgtggtaa cgggcaggat
    27121 cgctgatgtg ctggcgcaga ttgagcgcca ccatgcggtc gtctttgtta tagctgtcga
    27181 tacccgtaca ggtggagagc gcaatcacct gatagccttc atcgagaatg cgattgacca
    27241 ccccggcaaa ggctttttca tacgcttgtt gagtggtgcc gagacgtttg tcgaacggtg
    27301 ccagttcgcg cagggtaatt gccactgttt tctgttgtgc ggcaacgtcc agccagtgtt
    27361 gaacggcata gctggcggtg aagtcttcgg tgtggtgatc gaccagccac gcggtatcga
    27421 cgccatgttc cacttttgcg gtggtgatat ttctgcgttt catcaaatca aggctgaccg
    27481 attcgcgcag gatcagcgcg tcgcagtgac caaagacata gttcgccagt tggttaaatt
    27541 gctcatcctg gaaggggccg acgctgtgac caatcataaa cagcggcttt ttcgccataa
    27601 atgtgcaaag cgcatgttca aactgcggca cgccgtagag atcgacaaaa aacgatccgc
    27661 cgacctgaat aatggcgtcg taacctgaca gcaggcgcac gaagtcggtg aatccctggg
    27721 cgatggcgat attgcgcagc ttgccagtgt cggtgacgcg tgagagcaat acctgatgct
    27781 ggtagcggcg acggaggact tttttaatgc gcccgacaac gcccgccgcg ctattgtgtt
    27841 gtgtcatctg caggaacagc ggatcgccca ttaccgggcg gttgagcagc caggaagaac
    27901 tgaccggata gcggctcatc acgtccactt cggtatgtgg attgaggatg ttgatggcat
    27961 caagtaagcc gcgcaagatg gcgctgtcgc cacgattgcc gcaagtgtgg ttgcccagaa
    28021 taagtaattt cattttttcc tcataaattt gatgccaggt gaggctgtgt ttgttgtgtt
    28081 cgttgccgtt ttttgcctga tgcgacgctg gcgcgtctta tcaggcctac aggtcccgag
    28141 cacagaaccg taggacggat aaggcgtttt acgccgcatc cggcaaccgt tgtcggaacc
    28201 gaaaacagca attcaccccg cccgtaaaag cattttcatt ttttcgctgc gacaaaactg
    28261 acgcttcact tccaccacca gcggatggcg tgacagcaca atcatcacca caaacgccag
    28321 cacgcccgcg gctatttgca ccgccagcag catccccagc gccagttgcc ctttcagcac
    28381 aatgcccagc gcataactga ccaccagcgt cggcaacgag agataaaacg gcagccacaa
    28441 actcaggatg tactggcgat aactggaacc aagaaccggt ttaatcatca cgaagtaact
    28501 cagaatggtg ttgataatct gcaccagcag gaaaccgagc gtgacgccga ttgcgcccgc
    28561 catctgccca ccaataacaa tcgccggaat aaacagaaat gttttgaata cgttgaattt
    28621 aaagctgata tcgacccgcg ctttcgccat cagcagcgaa ccaatcgggt tacccacgga
    28681 gcgcagcaga cccacaatac acagcaattg cagcaccgga ataatgctgt tccacttttc
    28741 gccgaatacc agcggtacga agttattcga caccaccatc agccccagca gcgccggaaa
    28801 gttgataatc cccactatcg acagcagctt gtagaagtta acacgcagct tttcggtgtc
    28861 gtcctgaatt ttggcaaacg ccggaaacaa cacgcgggtg atgattgggt tcagcttcat
    28921 cggcggtacg acggccacgt tgtacgccag gttgtatccc cctgccacgc ccgcgccaag
    28981 aatacgcgcc agcaccagcg ttgaaaggtt ggtgttgaga tagttgatga tgctgtccgc
    29041 cgtcagccag gcaccaaaac gtaagttcgg tgccacagac gccagcgaga agtgcagacc
    29101 ggggcaataa attttgcgtc caaagtagcc aaacagcagt gttctcaccg cactattgac
    29161 cagataaccg aggatcgcgg tcatcgccag cggccagaaa tgggcgctaa ctaccgtaaa
    29221 agtgaagccc gccagcaccg cgctggtttc gatcatgccg attttgttga actccagctc
    29281 cttctgcatc aacgcgcgga actgttgccc gtgggggatc accacaaacg ccagcgacaa
    29341 tgttttaatc aacggtgcca ggtccgggtt attcagcacg tcgccgatga catcactcaa
    29401 caaaaacacc gccacacata ccacaagccc cagcccgacg ttcagccagt acagcgtggt
    29461 gagttcaagg tgactgattt cttttcgctg aataatcgag ttagcgatac cgaagtcaga
    29521 aagcgtatct gccagcgcga taatcaccag cgacacagtc agcaggccga actggtggtt
    29581 gtcgataatc cgcgccagca cggtcatctg caccagcccg aggccgatga tgatcaccgt
    29641 ggcaatagcc gaccacttcg cgccgctgat ggttttttca cgtaagctca tatcaatatg
    29701 ccgctttgtt aacgaagccc ttgaataccg tcaggaaaac gattttgata tcgaaccaga
    29761 cgctccattc gcggatgtac tcaaggtcga actcgacgcg tttttccatt ttctccagcg
    29821 tgtcggtttc gccgcgccag ccattaatct gcgcccagcc ggtaatgccc ggtttcactt
    29881 tatggcgcag catgtagcct tcaatgagct ggcgatactg ttcgttatgc gctactgcgt
    29941 gcggacgtgg accgacaatc gacatccccc cggtcagcac attgataaac tgcggcaatt
    30001 cgtccagcga ggtgcggcgc agaaagttcc ccactttagt gacgcgcgga tcgttctgcg
    30061 tcgcctgggt caccactttg tcgttctcca tcactttcat ggaacggaac ttccacactt
    30121 tgatcggctt gccatccata ccatagcgag tctggcggaa aataaccggc cctggtgaac
    30181 tgagtttcac cgccagcgca atacagcaca gcaccgggga gatcagcagc agaataagcg
    30241 tcgccagcac aatgtcttcc gcacgtttta gcaggcggtt aacccccgaa agcggcgtgt
    30301 catacagcgg caccaccgga acgccgttca tctcttcgag acgtgaatgg agaatgttga
    30361 aggtaaagac gtcggggatc agcagcaccg aacaggtggt atccgccagt tgatggacca
    30421 gttttttcac tcgtgcgccg tcgcacattt gcatcgcgat atagacgtta tgaatcttgc
    30481 ccgcttttgc atcctcgacc agctgttgca ggttacccgc ccagtcgtta gaaacgccgc
    30541 ccggtttcgg gtcgtggtaa acgcccacca cttcaaaccc taaccacggc tgattacgga
    30601 aactctccat cagcatttgt ccggcggcta aatcccccgc tacggcgacc atccgcttgt
    30661 tgtagccatg attacgcagc cagcccgccc caatgcgaat acatgaacgg caaaccacca
    30721 gtccgatgct ggtcagccca taccacgcca gccagatttt cagttgcgtg tcgaaatcat
    30781 tgttgaacgc caccagtccg gcgctgaaaa tcacgcttaa ggtccagttt tgtagcaaca
    30841 gggcaaattc tgtcgctgcc cgaacaccgc gccatgagcg ataaaaatcg gtgatgccgc
    30901 ccagcatctg gaacaccacc agcgtaatca gcgccaccaa caggtgcatg tagaggaatg
    30961 acagtccgct gacttcgcaa accagccata gcccggcaaa catgatggtg atatctgaaa
    31021 agcgttgcac catagagatt aacgatgcat tggttttcgc tcgctcgcgc ttttttagat
    31081 ttgtcatcgt tgttcctgtt attagcccct tacccgcagt ggggtaaggg aagatccgac
    31141 attactcgtt cagcaacgtc agcagagttc gcgttcgcgc ttccatcagc ggcacatcac
    31201 cgcgcgattc cacattcagg cgcaccaccg gttcggtatt ggaggtgcgc aggttaaagc
    31261 gccagtcggc aaaggtcatg ctgatgccat cgctgcgatc caccgccagc gcctcacggc
    31321 taaaatgctg ttccacgcgg ttaatcgcct caacgggttg cgccagtttg ctgttgatct
    31381 caccgcttgc cggaaacgcc gccatccggt cgcgcaccag ttcgcccagc gtttttcctt
    31441 tcaggcacac cagttcggcg accagcagcc acgggatcat gccgctgtcg cagtaagcga
    31501 aatcacggaa gtaatggtgg gcgctcattt cgccaccgta gatggcgtct tccttgcgca
    31561 tccgttcttt aataaaggcg tgtccggttt tcgacattac cggcgtgccg cctgcagcag
    31621 tcaccacatc aatagtgttc caagagagac gcggatcatg gatgatcttc gcgccgggat
    31681 ttttttcgag gaacgcttct gccagcaggc cgacaatgta gtagccctcg ataaactgcc
    31741 ctttttcgtc aaacagaaaa cagcggtcaa aatcgccatc aaaggcaatg cccatatccg
    31801 cgccgtgttt gatgaccgca ttgcgggtgt cgtcgcgaca ttccggcagc agcgggttag
    31861 gaataccgtt ggggaaattg ccgtccggtg tgttgtgtac tttgattaat tccacgggcg
    31921 cgccgagggc tttaaagcgg gcttcaatgg cgtccaccac cggacccgct gcgccattcc
    31981 cggagttgat caccagcttg agcggcgtga gatttttgac attgatataa ccgaacaggt
    32041 gatcaacgta agcgtcacgc aggttgattt gctgatagcg accgcgtttg gtttcatcga
    32101 cgggaggaaa gtcgttggct tcagccagac gctggacgtc gcgcagtccg gtatcaccgc
    32161 tgatcgtgcg agccccctcg cgcaccagct tcatgccgtt gtaatccatc ggattatggc
    32221 tggcagtaac ttcaatgccg ccatccacgc cgagatggaa cgtggcgaaa tagatctctt
    32281 cggtgccgga cataccgata tccagcacat cgacgcccgc atcctgtaaa ccttttgcca
    32341 gcgccagttt taaggtttcg ctggtgagac ggacatcacc gcctaacaca atggttttcg
    32401 gtttgagaaa ttcgccatag gcgcgaccaa tgcgccaggc gatatcttca ttcagttctt
    32461 cgcctaattt cccgcgaata tcataggctt taaagcaggt taattttttc atgtcgttac
    32521 cctttttcag gcaatagttg gccctgaccg aaacggccaa ttttattttt gtcgttattt
    32581 acaccgcggt ttcgcattca ttgcctgatg cgacgctgcc gcgtcttatc aggcctacaa
    32641 aaccgaaccg taggtcggat aaggcgttca cgccgcatcc gacaaacagc gcctgatgcg
    32701 acgtttacac ccgtccgtag cgatccgcga agcgcaccac atcatcctct tcgagataag
    32761 agccggagcg cacttcaatt aaatcgagcg gaattttccc cgggttttcc aggcagtgcg
    32821 tcgcccccag cggaatataa atggactcgt tttcaccaag cagtttgata tcaccatcaa
    32881 tggtgacttt tgccgttccc gcgaccacta cccagtgttc cgcgcggtga tggtgcatct
    32941 gtaccgacaa accctcgccc ggtttcacgg tgatgcgttt cacctggtag cggtcgcccg
    33001 cgtcgataga gtcatatttg ccccacggac gatacacttc gcgatgcacc cgatgctcat
    33061 ggcgaccatc ggctttgatc tgctcgacca cttttttcac atcctgtacc gcgttacggt
    33121 cggcaatcag caccgcatct ttggtctgca ccactaccag atctttcacc ccgacggtgg
    33181 tgaccaggcc tgattcggca tacacatagc tgttttcagt tttgtgatta atcacatcgc
    33241 cgtggcaaac gttgccctcg gcggtgtggg cgctgatctc ccataatgaa gaccaggaac
    33301 caacatcgct ccagcccgca tccatcggca ccacaacagc atctgccgta ggttccatga
    33361 ccgcgtaatc caccgactct tccgggcagg cgagaaacgc ttcttcatcc acacgaataa
    33421 aatcgagatc cggatcgacg gcgctcatcg ctttttcgca ggcatcgagg atatccgggc
    33481 gatatttttt cagttcttcg agatagcgtc cggcgcggaa caggaacatg ccgctgttcc
    33541 agtaatattc gccgcttgct acataggcct gggcggtttc cagattcggt ttttcgacaa
    33601 actgcgccac ttcaaaggcc accgtatcct gctcacccac agacacttca ccgcgacgaa
    33661 tatagccata accggtttct ggtagatccg gcacaatgcc aaaggtcacc agcttgcccg
    33721 cttcggcata aggcatggca ttacgcacgg cggcacggaa cgcgtcttca tcggcaatca
    33781 catgatccgc cgccaatacc agcattaacg ggtcgctctc cgggctatga cgttttgccg
    33841 ccagcgccgc cagtgcaatg gcaggtgcag tgttacgccc tgccggttcg agaataatgt
    33901 tctcggtgag tttgttcagt tgacgcagct gttccgcgac aataaagcgg tgctgctcat
    33961 tgcaaatcac caccgggctt tcgcactcca cgccgttcag gcggcagatg gtggtttgca
    34021 gcatggtgag atcgcctttc aggcataaaa actgcttggg gtaaagtacg cgggaaagcg
    34081 gccataaacg gctaccggag ccacctgcca tcacaactgg atagagtttc gactgcgcca
    34141 taattatccc cgaatatcat ttataaattg acgtaacacg ttctctttat cgagcgtgcg
    34201 ttcggcatat tcacgtgcca ccgtgttatg tttgggcagc aggagcgcct gacgaatccc
    34261 cgccaccagc gcctcgaccg attccggttc tacgcaaacc gcaatacccg gaaaggtttc
    34321 gcaaagctgc cccagttctg tgtgggcctc tgcggtaatc accgcgttac cgcctaccgc
    34381 cagaatattg gtcagtttcg acggcaatac ggcatctgcc gcgccgcgtt tttgcaccac
    34441 cagatggcaa tcgcccatct tcagcagtgc aggtaaagcg tcatacgatt gcagcgggaa
    34501 aaattgcatg ttgcgcagtc cacgctgctg cgccattttt tccagccgcg ctttgccgcc
    34561 gccctgcccg acaatggcaa aaatcagcgg ttcatcgcgc aggagatcgg cagcttcaat
    34621 aacgttttcc agcccctgct tttcaccaat attgccggag taaagaatga tttttttgtt
    34681 atccggcagg ccaagctggt tacgaagggc atcaacatcg gcgtctgcaa catgctgaaa
    34741 acgggcaatt tccgaccagt tggggaagaa gatgacgtta tccgccgcca cgcctttttc
    34801 gatggcttta ttcatcatcg aacgcgaaat cgtggaaacg ttatcgacgt tatgcagtcc
    34861 gctacgttcg aacgccgttg ccagctgtgc cactttgccg cctttgcctt ttccggcaag
    34921 gcccagcccc agcatggcgt ccacttcgta atcctgaata tgcagcacgg tacgcgcacc
    34981 agagagcttc gccagcaggc gcattcccgg cgtgcaaaac agcgttggca ctacgccaat
    35041 aatgcgatcc ggcttccagc gacgttgcgc catcagtgga aagaaactgc tgacggcaaa
    35101 actgccgaga tgcaacaagc gtttcagggt gctcggctgt tttggcacgt acagcgggca
    35161 gcgccacacc gtggcggccc cttcttctcg tttgtagcgc caggcggaat agttctcacc
    35221 cacctgccat tgcgggtagt aaggcggtgc ggtaatgacc cgcacctcat gaccttgtgc
    35281 cgccaaccat tccaccatct cgccggtgta tttgccgatg ccggttaact ccggcgagta
    35341 gttaatgccg tagaccagta ttttcataat ccgggtactc cggcacgctt ctcagcgaga
    35401 aaataggctc ggctgttagc gtgaacatcg ttactggcga gcagcgcgtc cggcgtcagc
    35461 cagcggtaat cgtcatgctg ctcatccggc agtaacagct cttcttccgc tactctgaag
    35521 cgaaaaccga gcaccacata gtgagtggtg aaatccgtgc cggagaagtt atcgtcataa
    35581 aaatgctgcc agacaccgta aaactggcct gctgttatcg gcagacgcag ccccagttcc
    35641 gccatcgtca gccgctcaaa tgcggcttcc agcgtttcgt ctttctgcac gcgccctccc
    35701 ggcacaaacc agtaaccctg cgccgggcgg ttggttcttt tgccaagcag aaactcgccg
    35761 cgactgttct cgacaataaa gtcgagagag acaagcggtg tggagcgcac tacagtggca
    35821 aagtcttcct gacgtaaaaa catcattccc cccgaaagcg gtcttgattc tcaaggaacc
    35881 actggtaagt gctggcaagc cccgcttcca gtgagatttc gtgataccag ccaagctgat
    35941 gcaggcgcgt cacatccagc agtttgcgcg gcgtaccatc cggtttgctg gcatcaaaaa
    36001 ccacccgacc tttgtaaccc accactttgg cgatggtttg cgccagctcg cggatagtgc
    36061 agtcaacgcc cgtgccgacg ttaatgtgcg acagcatcgg ttgggtattc tccagccaga
    36121 cttcatgcgc cagctccatg acatgaatgc tcgccgccgc catatcatcg acgtgcagga
    36181 attcacgcat cggtgtaccg ctgccccata ccaccacgtc cggcgcattc tgtgccgtcg
    36241 cctcgtggaa gcgacgcagc aatgctggta tcacatggga gttactcggg tggaagttgt
    36301 catgcggccc gtacaggttg gtcggcatga cagtgcggta atcgcgtcca tactggcggt
    36361 tgtaagattc gcacagtttg atcccggcga ttttggcaat ggcgtaaggc tcgttggtcg
    36421 gctccagcgt gccctgcaat aactcgcttt ctgccatcgg ctgttttgcc agtttcgggt
    36481 agatgcagga cgatccgaga aacatcagtt tgttcacgtc gttctgatgc gcggcgtgaa
    36541 tgatgttgct ctcaatcatc atgttctggt agatgaaatc cgccggatag gtgttgttgg
    36601 caacaatgcc gcccactttc gccgccgcca gatagacctg gtcaatgcgt tcgctggcaa
    36661 agaaatcatg cacggcgcgg ctgtccagca ggttcagttc atcgcgggta cgtaatacca
    36721 gctccacatc accgcgctgt tcgagctgcc gcttgatggc agaaccgacc atcccacaat
    36781 ggccagcgat aaaaatgcgt tgtttactca tgcttatgac tccagcgcga tcgccacgtc
    36841 gtagccgtga gatttcagca gagagtgttt tttcgccgct tcgaggtcat tagccaccat
    36901 ttcagacacc atctctctga gggtgatttc cggtttccag cccagttttt cgtgcgcttt
    36961 ggtcgggtcg ccgagcagcg tttcaacttc tgccggacgg aagtaacgcg gatcaacggc
    37021 gataatcaca tcacccggtt taacgcccgg cgcgtcatgc ccggtgacgg aaaccacaat
    37081 gcccttctct tcaacacccg tgccttcaaa gcgcagtttg atgcccagct gtgctgccgc
    37141 catttccaca aactgacgta cggagtactg aacgccggtc gcgataacga aatcttccgg
    37201 ctgttcctgt tgcagcatca tccactgcat ttttacgtag tctttggcgt ggccccagtc
    37261 acgcagggaa tccatattgc cgaggtacag gcacgactcc agcccctggg cgatgttggc
    37321 gattgcgcgg gtgattttgc gggtaacgaa ggtttcgccg cggcgcgggg attcatggtt
    37381 gaagagaatt ccgttacaag cgtacatgcc gtaggattcg cggtagttaa cggtgatcca
    37441 gtaggcgtac agtttggcga ccgcatacgg agatcgcggg tagaacggcg tggtttcttt
    37501 ctgcggaatt tcctgcacca gaccatacag ttcagaggtg gaagcctgat agaaacgagt
    37561 tttcttttcc agaccgagga agcggatcgc ctccagcagg cgcagcgtac ccatcgcgtc
    37621 gacgtcagcg gtatattctg gtgactcaaa agagaccgca acgtggctca ttgcgcctag
    37681 gttgtacact tcatccggct gtacttcacg caaaatgcgc gtcaggttag aggtatcact
    37741 caggtcgcca taatgcagat ggaatttcgg gttgcaggtg tgcggatcct gataaatgtg
    37801 atccacgcgc tcggtgttga atgacgatgc gcgacgctta ataccatgca cctcgtaacc
    37861 tttttccagc agaaactctg ccaggtaaga accgtcttgt ccggttacac cggtgatgag
    37921 agcgactttt gacatgtctt attcctctgt atttttgaat ttattcagtt tcaacgcgtt
    37981 cgcgtatcac cactgcggga ttcccccggc aaaccacatt tgccggaagc gatttaaaaa
    38041 cactgcttcg tgcacccacg acggtgccgt cgccgattgt gacgcctggg gcaacaaaga
    38101 catcggttgc cagccagcat ttctcgccaa tcacaatagg cgtggcgtta atggtgaaat
    38161 gttgacttgc atggtcgtgg ctaccggtgc ataaataact tttttgcgat atcaccgaat
    38221 gtgcgccaat ggttatttca ccgagggtat ataaattgac gtcatcgcca acccacgcgt
    38281 aatcacctaa ggttaatttc cacggatagg taatttttac tgacggacga ataactacgt
    38341 tttttcctat ttttgctccg aataaacgta ataaaaaagc ccgccagcga tacaatactt
    38401 gtggcgacca ggcaaatatt gttgcctgta ctgcccacca taattgcact ttaatagcgt
    38461 tgccgccccg gaaccctttc ggcaccgaga aaccgcttaa atcttgcatt ggttttcctt
    38521 atattcagac tttgttatat aaggctttag tcttacctgt cgtacgttgg cgtaaatgcc
    38581 aggataattc tgcccagaag ccaggtacat gtaatatctg ccgttggact tttttggcgt
    38641 cagcacac
//
LOCUS       DAJTQQ010000030        37935 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000030 DAJTQQ010000000
VERSION     DAJTQQ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.030"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            84..1580
                     /gene="ravA"
                     /locus_tag="OO309_001700"
     CDS             84..1580
                     /gene="ravA"
                     /locus_tag="OO309_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR5846869.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1574..3025
                     /gene="viaA"
                     /locus_tag="OO309_001701"
     CDS             1574..3025
                     /gene="viaA"
                     /locus_tag="OO309_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR5846870.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3030..4022)
                     /gene="asnA"
                     /locus_tag="OO309_001702"
     CDS             complement(3030..4022)
                     /gene="asnA"
                     /locus_tag="OO309_001702"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR5846871.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4174..4632
                     /gene="asnC"
                     /locus_tag="OO309_001703"
     CDS             4174..4632
                     /gene="asnC"
                     /locus_tag="OO309_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR5846872.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4722..5165
                     /gene="mioC"
                     /locus_tag="OO309_001704"
     CDS             4722..5165
                     /gene="mioC"
                     /locus_tag="OO309_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR5846873.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5544..7433
                     /gene="mnmG"
                     /locus_tag="OO309_001705"
     CDS             5544..7433
                     /gene="mnmG"
                     /locus_tag="OO309_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR5846874.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7497..8120
                     /gene="rsmG"
                     /locus_tag="OO309_001706"
     CDS             7497..8120
                     /gene="rsmG"
                     /locus_tag="OO309_001706"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR5846875.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8737..9117
                     /gene="atpI"
                     /locus_tag="OO309_001707"
     CDS             8737..9117
                     /gene="atpI"
                     /locus_tag="OO309_001707"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR5846876.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9126..9941
                     /gene="atpB"
                     /locus_tag="OO309_001708"
     CDS             9126..9941
                     /gene="atpB"
                     /locus_tag="OO309_001708"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR5846877.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9988..10227
                     /gene="atpE"
                     /locus_tag="OO309_001709"
     CDS             9988..10227
                     /gene="atpE"
                     /locus_tag="OO309_001709"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR5846878.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10289..10759
                     /gene="atpF"
                     /locus_tag="OO309_001710"
     CDS             10289..10759
                     /gene="atpF"
                     /locus_tag="OO309_001710"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR5846879.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10774..11307
                     /gene="atpH"
                     /locus_tag="OO309_001711"
     CDS             10774..11307
                     /gene="atpH"
                     /locus_tag="OO309_001711"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR5846880.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11320..12861
                     /gene="atpA"
                     /locus_tag="OO309_001712"
     CDS             11320..12861
                     /gene="atpA"
                     /locus_tag="OO309_001712"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR5846881.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12912..13775
                     /gene="atpG"
                     /locus_tag="OO309_001713"
     CDS             12912..13775
                     /gene="atpG"
                     /locus_tag="OO309_001713"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR5846882.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13802..15184
                     /gene="atpD"
                     /locus_tag="OO309_001714"
     CDS             13802..15184
                     /gene="atpD"
                     /locus_tag="OO309_001714"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR5846883.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15205..15624
                     /gene="atpC"
                     /locus_tag="OO309_001715"
     CDS             15205..15624
                     /gene="atpC"
                     /locus_tag="OO309_001715"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR5846884.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15975..17345
                     /gene="glmU"
                     /locus_tag="OO309_001716"
     CDS             15975..17345
                     /gene="glmU"
                     /locus_tag="OO309_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR5846885.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17507..19336
                     /gene="glmS"
                     /locus_tag="OO309_001717"
     CDS             17507..19336
                     /gene="glmS"
                     /locus_tag="OO309_001717"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR5846886.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            19498..20319
                     /locus_tag="OO309_001718"
     CDS             19498..20319
                     /locus_tag="OO309_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCR5846887.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            20306..22414
                     /locus_tag="OO309_001719"
     CDS             20306..22414
                     /locus_tag="OO309_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR5846888.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            22411..24078
                     /locus_tag="OO309_001720"
     CDS             22411..24078
                     /locus_tag="OO309_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5846889.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            24081..25607
                     /locus_tag="OO309_001721"
     CDS             24081..25607
                     /locus_tag="OO309_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCR5846890.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            25608..27224
                     /locus_tag="OO309_001722"
     CDS             25608..27224
                     /locus_tag="OO309_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR5846891.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALELSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(27280..27432)
                     /locus_tag="OO309_001723"
                     /pseudo
     CDS             complement(27280..27432)
                     /locus_tag="OO309_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(27455..27832)
                     /locus_tag="OO309_001724"
     CDS             complement(27455..27832)
                     /locus_tag="OO309_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846892.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            complement(28242..28613)
                     /locus_tag="OO309_001725"
     CDS             complement(28242..28613)
                     /locus_tag="OO309_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846893.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(28674..29171)
                     /locus_tag="OO309_001726"
     CDS             complement(28674..29171)
                     /locus_tag="OO309_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846894.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(29247..30035)
                     /gene="aadA1"
                     /locus_tag="OO309_001727"
     CDS             complement(29247..30035)
                     /gene="aadA1"
                     /locus_tag="OO309_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5846895.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(30148..30978)
                     /locus_tag="OO309_001728"
     CDS             complement(30148..30978)
                     /locus_tag="OO309_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5846896.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            31188..32201
                     /gene="intI1"
                     /locus_tag="OO309_001729"
     CDS             31188..32201
                     /gene="intI1"
                     /locus_tag="OO309_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5846897.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            32404..32754
                     /locus_tag="OO309_001730"
     CDS             32404..32754
                     /locus_tag="OO309_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5846898.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            32880..33440
                     /locus_tag="OO309_001731"
     CDS             32880..33440
                     /locus_tag="OO309_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5846899.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            33443..36409
                     /locus_tag="OO309_001732"
     CDS             33443..36409
                     /locus_tag="OO309_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR5846900.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            36476..36853
                     /locus_tag="OO309_001733"
     CDS             36476..36853
                     /locus_tag="OO309_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5846901.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(37054..37713)
                     /locus_tag="OO309_001734"
     CDS             complement(37054..37713)
                     /locus_tag="OO309_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5846902.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         9483 a         9285 c         9859 g         9308 t
ORIGIN      
        1 tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac gcagaacttt
       61 tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa agaatttccc
      121 gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc cgcttgtgtt
      181 tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt attgccaaaa
      241 gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt gaatatctga
      301 tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag gcgctaaaag
      361 atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa atcgtctttc
      421 tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc gccattaacg
      481 agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta ctggtggcgg
      541 cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac cgcatgctga
      601 ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg ctgaccagtc
      661 aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat gaagaatatg
      721 aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt gagctgattt
      781 ttattctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg gatcgtcgct
      841 ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc agtgctgttg
      901 ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa agcctgaatt
      961 tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag caagggatgt
     1021 tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa caaagcgata
     1081 aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag cagtatcaac
     1141 ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg ttaaaactgc
     1201 atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag tggctgagta
     1261 aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa ctgaatctgg
     1321 aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc agtacgctgg
     1381 cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa ctggaagagc
     1441 ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa tgtctgttta
     1501 tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt gcacagattc
     1561 gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg tcagcgaaga
     1621 gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg cggtcttctt
     1681 tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct ggcgtgaggc
     1741 gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag aggtgatgtg
     1801 ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac cacagatcct
     1861 ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt tggttgatgc
     1921 taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc gtttaagtct
     1981 gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg aacaactgtt
     2041 aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc tcgcagataa
     2101 caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac gtggcgacta
     2161 tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga aacgcctggc
     2221 agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg cgcagatgga
     2281 aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg atggtctgca
     2341 acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag ggataacgga
     2401 actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct atcgcctgca
     2461 cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg gtacataaag attacgatga
     2521 acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg gcggctttaa
     2581 tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg ctgaaaaccg
     2641 gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt caggcccaca
     2701 aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca ccgatcttgc
     2761 cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg atgccgatgc
     2821 ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga gtaaagtgaa
     2881 agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg cacacggcaa
     2941 acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga tgcgaagccg
     3001 cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct ctcgcgaaca
     3061 gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag cagcagcata
     3121 gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc gcgcagcagc
     3181 gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag ctgatgcttc
     3241 agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc ttccagtacc
     3301 gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga cggggtgctc
     3361 cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa cttgccgcca
     3421 atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg ccctttggca
     3481 tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat ctgatccggc
     3541 aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt aattcccgcc
     3601 cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc gcccattacg
     3661 cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg gtcttcatcg
     3721 gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc gtgttgccct
     3781 aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc atcaggcaga
     3841 gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg cgtgccatcc
     3901 cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc ttccagttgg
     3961 cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta agcggttttc
     4021 attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg acacacatgc
     4081 aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga cggctaaaat
     4141 agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat cgacaatctg
     4201 gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc cgaactggcg
     4261 aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat gaagcaggcg
     4321 gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta tgacgtaggc
     4381 tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct ggcaaagctg
     4441 gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag catctttata
     4501 aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa gatccaaaca
     4561 attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat catgcgtacc
     4621 atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc ccaacgcgtt
     4681 cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat atcactctta
     4741 tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct gaaaagctgg
     4801 aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat ttatctgcct
     4861 cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg gacaaccttt
     4921 ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc cgctttggca
     4981 caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat aaactcgagg
     5041 cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc aacattcttg
     5101 atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt aatttactca
     5161 aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc ttggatcaac
     5221 cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa cccctcattc
     5281 tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat cctttccagg
     5341 ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc taataagaga
     5401 tcacaataga acagatctct aaataaatag atcttctttt taatacccag gatcccaggt
     5461 ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc cattttcata
     5521 ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc atcatcattg
     5581 gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt caacagactc
     5641 tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg gcgatcggcg
     5701 gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg atggcgaaag
     5761 cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga ccggcggttc
     5821 gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt acggcgctgg
     5881 agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt gtcgaaaacg
     5941 atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa gctgtggtgc
     6001 tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat tacagcggtg
     6061 gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa ctgccgctgc
     6121 gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc atcgacttta
     6181 gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt atgggcaatg
     6241 cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag aaaacccatg
     6301 atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc gaaggtgtcg
     6361 gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac agaaatcagc
     6421 atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg aacggtatct
     6481 ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag gggatggaaa
     6541 acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat cctcgcgacc
     6601 tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt cagattaacg
     6661 gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt aacgctgccc
     6721 gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat ctcggcgtac
     6781 tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt acttcgcgcg
     6841 cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact gaaatcggtc
     6901 gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa cttgagaata
     6961 tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg gaagctgcag
     7021 ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt gaagatctgc
     7081 tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt gcccctgcgt
     7141 tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa ggttatatcg
     7201 cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg ctacccgcga
     7261 cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa cttaacgatc
     7321 acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct gcggccatct
     7381 ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca taacgcatta
     7441 aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta atcaccgtgc
     7501 tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat caccagaaaa
     7561 accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac aacctgactt
     7621 cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt gtggtggcac
     7681 cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg ccaggcattc
     7741 cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt ggtaaacgcg
     7801 tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa ccagtacaga
     7861 gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc cgcgcttttg
     7921 cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa ggccgtttct
     7981 acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa gaatatcagg
     8041 tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat ctggtggtga
     8101 ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac cggttagact
     8161 gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc acgttcactc
     8221 ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt cattagtgaa
     8281 aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa acattattaa
     8341 aaatgtcaat gagtggtttt tgttgtgtaa atgttattta ttaaaacagt atctgttttt
     8401 agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa tatgctgtgc
     8461 gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta cgcgttattt
     8521 acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc actggaaaaa
     8581 tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg ggcgcaccgt
     8641 ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt atacgacacg
     8701 cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct cgtgagtcga
     8761 aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag tggattgctg
     8821 ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct ggcagtcttt
     8881 ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac accagcgaaa
     8941 ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc gatgttggtg
     9001 ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat cgttacgtgg
     9061 gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa agggtaaaag
     9121 gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac ctgaataacc
     9181 ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca gccaccttct
     9241 ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg ttcctggttt
     9301 tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt cagaccgcga
     9361 ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat ggcaaaagca
     9421 agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg aatctgatgg
     9481 atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt ctgcctgcac
     9541 tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg ggcgtattta
     9601 tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg aaagagttga
     9661 cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt gaaggggtaa
     9721 gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg tatgccggtg
     9781 agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg atcctgaatg
     9841 tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc ttcatggttc
     9901 tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac actactacgt
     9961 tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct gctgtacatg
    10021 gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat cggcatcctc
    10081 gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct gctgcgtact
    10141 cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt aggtctgggt
    10201 ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga gcaatatcag
    10261 aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc tcggccaggc
    10321 catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc cattaatggc
    10381 agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag aacgagcaca
    10441 taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag cgaaagcgga
    10501 agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg acgaagcgaa
    10561 agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag aaattgaagc
    10621 cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg ctgttgctgg
    10681 cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca tcgtggataa
    10741 acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac ggtagctcgc
    10801 ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga acgctggcag
    10861 gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga gcttctctct
    10921 ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg tgagcaactg
    10981 gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct taacgcgctc
    11041 ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac cgctgaggta
    11101 gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc tgctgcgatg
    11161 gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc tgtaatggct
    11221 ggcgttatca tccgatcggg tgatatggtc attgatggca gcgtacgcgg tcgtcttgag
    11281 cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa ttccaccgaa
    11341 atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga agctcacaac
    11401 gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct ggccgattgt
    11461 atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact gaacctcgag
    11521 cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga aggcatgaaa
    11581 gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct gggccgtgtg
    11641 gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga cggcttctct
    11701 gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca gccggtacag
    11761 accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg tgaattgatc
    11821 atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat caaccagcgc
    11881 gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac catttctaac
    11941 gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt ggtagcaacc
    12001 gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc aatgggcgaa
    12061 tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc taaacaggct
    12121 gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga agcattcccg
    12181 ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt taacgccgaa
    12241 tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct gaccgcactg
    12301 ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa cgtaatctcc
    12361 attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat tcgtcctgcg
    12421 gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa gatcatgaaa
    12481 aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc agcgttctct
    12541 cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg tcagaaagtg
    12601 accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca gtctctggtt
    12661 ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat tggcagcttc
    12721 gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca agagatcaac
    12781 cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct cgattccttc
    12841 aaagcaaccc aatcctggta acgtctggag gcttgcctta gggcaggccg caaggcattg
    12901 aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc gtccagaaca
    12961 cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt aaatcgcagg
    13021 atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt ggtcaccttg
    13081 cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt aaacgcgtgg
    13141 gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt aacctgttca
    13201 aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc gacctcgcaa
    13261 tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt gttgcccagg
    13321 tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta aaagtgatgt
    13381 tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac aaatttatta
    13441 acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca tcagatgatg
    13501 atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag gcgttgctgg
    13561 ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt gaaaacctgg
    13621 ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat ggcggcagcc
    13681 tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt actcaggaac
    13741 tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta gaggatttaa
    13801 gatggctact ggaaagattg tccaggtaat cggcgccgta gttgacgtcg aattccctca
    13861 ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg agcgtctggt
    13921 gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa tgggttcctc
    13981 cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg aagtcccggt
    14041 aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg acatgaaagg
    14101 cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct acgaagagct
    14161 gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga tgtgtccgtt
    14221 cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa ccgtaaacat
    14281 gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt ttgcgggcgt
    14341 aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact ccaacgttat
    14401 cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc gtctgcgcgt
    14461 tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg acgttctgct
    14521 gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg cactgctggg
    14581 ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg gcgttctgca
    14641 ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg tatacgtacc
    14701 tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg acgcaaccgt
    14761 ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc cgctggactc
    14821 caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca ccgcgcgtgg
    14881 cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca tcctgggtat
    14941 ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga tccagcgctt
    15001 cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta aatacgtctc
    15061 cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg atcacctgcc
    15121 ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag ccaaaaaact
    15181 ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt cgtcagcgca
    15241 gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag cgaaggtgaa
    15301 ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg tatgattcgc
    15361 atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat tcttgaagtg
    15421 cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga tctcgacgaa
    15481 gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc tcacggcgac
    15541 gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct gcgcgttatc
    15601 gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca gtctggaaac
    15661 aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga tcctgctgat
    15721 ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa aacatccacc
    15781 tgacgcttaa atgaaggtac tgccttaatt ttctgcagat aaaaggcgtg acgatggtcg
    15841 aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc tctgtccatt
    15901 tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt tctcaaatta
    15961 cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc gcaggcaaag
    16021 gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg aaagcgatgg
    16081 ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac ctggtgtacg
    16141 gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac tgggtgcttc
    16201 aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc tttgccgatg
    16261 atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa acactccagc
    16321 gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa ctggatgatc
    16381 cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt gttgagcaca
    16441 aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt ctgattgcca
    16501 acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct cagggcgaat
    16561 actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc gtcgccgttc
    16621 atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc tcccgtctgg
    16681 agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt atgctgcgcg
    16741 atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt gaaattgata
    16801 ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc ggcaccggtt
    16861 gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat accgttgtgg
    16921 aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg cgtcctggtg
    16981 ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa gcgcgtctgg
    17041 gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt ggcgataacg
    17101 ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa tttaagacca
    17161 ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg gtaacagtag
    17221 gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc gaaaatgcat
    17281 tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg gtaaagaaaa
    17341 agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa tctccctccc
    17401 acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc ccgaaaaaac
    17461 gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt gtggaattgt
    17521 tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac gtcgtctgga
    17581 ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc atatgactcg
    17641 cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac atcctctgca
    17701 tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt cagaagctaa
    17761 cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca tcgaaaacca
    17821 tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg aaaccgacac
    17881 cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc tgcgtgaggc
    17941 tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca tggactcccg
    18001 tcacccggat accctgctag cggcacgttc tggtagtccg ctggtgattg gcctgggtat
    18061 gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc gtcgctttat
    18121 cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca tcttcgataa
    18181 aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg acgcgggcga
    18241 taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga acgcgataaa
    18301 aaacaccctt accgggcgca tcagccacgg tcaggttgat ttaagcgagc tgggaccgaa
    18361 cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg gtacttctta
    18421 taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc cgtgcgacgt
    18481 cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca gcctgatgat
    18541 caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc tgtcgaaaga
    18601 gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc tggtgcgcga
    18661 atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt ccactaaagc
    18721 attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc gcctgaaagg
    18781 tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc cgagccgtat
    18841 tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt tctctgacaa
    18901 acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg aaggcgcatt
    18961 gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg agctgaaaca
    19021 cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac cgaacaacga
    19081 actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg gtcaattgta
    19141 tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca tcatcgagat
    19201 gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc agctgctggc
    19261 ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc tggcaaaatc
    19321 ggttacggtt gagtaatcac tatcatagat aaaaaagggc tgctgtgggc ggacaataaa
    19381 gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac tagacagaat
    19441 agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac ttttgttatg
    19501 gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa agaggggcgt
    19561 ggccaagggc atggtaaaga ctatattcca tggctaacag tacaagaagt tccttcttca
    19621 ggtcgttccc accgtattta ttctcataag acgggacgag tccatcattt gctatctgac
    19681 ttagagcttg ctgtttttct cagtcttgag tgggagagca gcgtgctaga tatacgcgag
    19741 cagttcccct tattacctag tgataccagg cagattgcaa tagatagtgg tattaagcat
    19801 cctgttattc gtggtgtaga tcaggttatg tctactgatt ttttagtgga ctgcaaagat
    19861 ggtccttttg agcagtttgc tattcaagtc aaacctgcag cagccttaca agacgagcgt
    19921 accttagaaa aactagaact agagcgtcgc tattggcagc aaaagcaaat tccttggttc
    19981 atttttactg ataaagaaat aaatcccgta gtaaaagaaa atattgaatg gctttattca
    20041 gtgaaaacag aagaagtttc tgcggagctt ttagcacaac tatccccatt ggcccatatc
    20101 ctgcaagaaa aaggagatga aaacattatc aatgtctgta agcaggttga tattgcttat
    20161 gatttggagt taggcaaaac attgagtgag atacgagcct taaccgcaaa tggttttatt
    20221 aagttcaata tttataagtc tttcagggca aataagtgtg cagatctctg tattagccaa
    20281 gtagtgaata tggaggagtt gcgctatgtg gcaaattaat gaggttgtgc tatttgataa
    20341 tgatccgtat cgcattttgg ctatagagga tggccaagtt gtctggatgc aaataagcgc
    20401 tgataaagga gttccacaag ctagggctga gttgttgcta atgcagtatt tagatgaagg
    20461 ccgcttagtt agaactgatg acccttatgt acatcttgat ttagaagagc cgtctgtaga
    20521 ttctgtcagc ttccagaagc gcgaggagga ttatcgaaaa attcttccta ttattaatag
    20581 taaggatcgt ttcgacccta aagtcagaag cgaactcgtt gagcatgtgg tccaagaaca
    20641 taaggttact aaggctacag tttataagtt gttacgccgt tactggcagc gtggtcaaac
    20701 gcctaatgca ttaattcctg actacaaaaa cagcggtgca ccaggggaaa gacgttcagc
    20761 gacaggaaca gcaaagattg gccgagccag agaatatggt aagggtgaag gaaccaaggt
    20821 aacgcccgag attgaacgcc tttttaggtt gaccatagaa aagcacctgt taaatcaaaa
    20881 aggtacaaag accaccgttg cctatagacg atttgtggac ttgtttgctc agtattttcc
    20941 tcgcattccc caagaggatt acccaacact acgtcagttt cgttattttt atgatcgaga
    21001 ataccctaaa gctcagcgct taaagtctag agttaaagca ggggtatata aaaaagacgt
    21061 acgaccctta agtagtacag ccacttctca ggcgttaggc cctgggagtc gttatgagat
    21121 tgatgccacg attgctgata tttatttagt ggatcatcat gatcgccaaa aaatcatagg
    21181 aagaccaacg ctttacattg tgattgatgt gtttagtcgg atgatcacgg gcttttatat
    21241 cggctttgaa aatccgtctt atgtggtggc gatgcaggct tttgtaaatg cttgctctga
    21301 caaaacggcc atttgtgccc agcatgatat tgagattagt agctcagact ggccgtgtgt
    21361 aggtttgcca gatgtgttgc tagcggaccg tggcgaatta atgagtcatc aggtcgaagc
    21421 cttagtttct agttttaatg tgcgagtgga aagtgctcca cctagacgtg gcgatgctaa
    21481 aggcatagtg gaaagcactt ttagaacact acaagccgag tttaagtcct ttgcacctgg
    21541 cattgtagag ggcagtcgga tcaaaagcca tggtgaaaca gactataggt tagatgcatc
    21601 tctgtcggta tttgagttca cacaaattat tttgcgtacg atcttattca gaaataacca
    21661 tctggtgatg gataaatacg atcgagatgc tgattttcct acagatttac cgtctattcc
    21721 tgtccagcta tggcaatggg gtatgcagca tcgtacaggt agtttaaggg ctgtggagca
    21781 agagcagttg cgagtagcgt tactgcctcg ccgaaaggtc tctatttctt catttggcgt
    21841 taatttgtgg ggtttgtatt actcggggtc agagattctg cgtgagggtt ggttgcagcg
    21901 gagcactgat atagctagac ctcaacattt agaagcggct tatgacccag tgctggttga
    21961 tacgatttat ttgtttccgc aagttggcag ccgtgtattt tggcgctgta atctgacgga
    22021 acgtagtcgg cagtttaaag gtctctcatt ttgggaggtt tgggatatac aagcacaaga
    22081 aaaacacaat aaagccaatg cgaagcagga tgagttaact aaacgcaggg agcttgaggc
    22141 gtttattcag caaaccattc agaaagcgaa taagttaacg cccagtacta ctgagcccaa
    22201 atcaacacgc attaagcaga ttaaaactaa taaaaaagaa gccgtgacct cggagcgtaa
    22261 aaaacgtgcg gagcatttga agccaagctc ttcaggtgat gaggctaaag ttattccttt
    22321 caacgcagtg gaagcggatg atcaagaaga ttacagccta cccacatacg tgcctgaatt
    22381 atttcaggat ccaccagaaa aggatgagtc atgagtgcta cccggattca agcagtttat
    22441 cgtgatacgg gggtagaggc ttatcgtgat aatcctttta tcgaggcctt accaccatta
    22501 caagagtcag tgaatagtgc tgcatcactg aaatcctctt tacagcttac ttcctctgac
    22561 ttgcaaaagt cccgtgttat cagagctcat accatttgtc gtattccaga tgactatttt
    22621 cagccattag gtacgcattt gctactaagt gagcgtattt cggtcatgat tcgaggtggc
    22681 tacgtaggca gaaatcctaa aacaggagat ttacaaaagc atttacaaaa tggttatgag
    22741 cgtgttcaaa cgggagagtt ggagacattt cgctttgagg aggcacgatc tacggcacaa
    22801 agcttattgt taattggttg ttctggtagt gggaagacga cctctcttca tcgtattcta
    22861 gccacgtatc ctcaggtgat ttaccatcgt gaactcaatg tagagcaggt ggtgtatttg
    22921 aaaatagact gctcgcataa tggttcgcta aaagaaatct gcttgaattt tttcagagcg
    22981 ttggatcgag ccttgggctc gaactatgag cgtcgttatg gcttaaaacg tcatggtata
    23041 gaaaccatgt tggctttgat gtcgcaaata gccaatgcac atgctttagg gttgttggtt
    23101 attgatgaaa ttcagcattt aagccgctct cgttcgggtg gatctcaaga gatgctgaac
    23161 ttttttgtga cgatggtgaa tattattggc gtaccagtga tgttgattgg tacccctaaa
    23221 gcacgagaga tttttgaggc tgatttgcgg tctgcacgta gaggggcagg gtttggagct
    23281 atattctggg atcctataca acaaacgcaa cgtggaaagc ccaatcaaga gtggatcgct
    23341 tttacggata atctttggca attacagctt ttacaacgca aagatgcgct gttatcggat
    23401 gaggtccgtg atgtgtggta tgagctaagc caaggagtga tggacattgt agtaaaactt
    23461 tttgtactcg ctcagctccg tgcgctagct ttaggcaatg agcgtattac cgctggttta
    23521 ttgcggcaag tgtatcaaga tgagttaaag cctgtgcacc ccatgctaga ggcattacgc
    23581 tcgggtatcc cagaacgcat tgctcgttat tctgatctag tcgttcccga gattgataaa
    23641 cggttaatcc aacttcagct agatatcgca gcgatacaag aacaaacacc agaagaaaaa
    23701 gcccttcaag agttagatac cgaagatcag cgtcatttat atctgatgct gaaagaggat
    23761 tacgattcaa gcctgttaat tcccactatt aaaaaagcgt ttagccagaa tccaacgatg
    23821 acaagacaaa agttactgcc tcttgttttg cagtggttga tggaaggcga aacggtagtg
    23881 tcagaactag aaaagccctc caagagtaaa aaggtttcgg ctataaaggt agtcaagccc
    23941 agcgactggg atagcttgcc tgatacggat ttacgttata tctattcaca acgccaacct
    24001 gaaaaaacca tgcatgaacg gttaaaaggg aaaggggtaa tagtggatat ggcgagctta
    24061 tttaaacaag caggttagcc atgagaaact ttcctgttcc gtactcgaat gagctgattt
    24121 atagcactat tgcacgggca ggcgtttatc aagggattgt tagtcctaag cagctgttgg
    24181 atgaggtgta tggcaaccgc aaggtggtcg ctaccttagg tctgccctcg catttaggtg
    24241 tgatagcaag acatctacat caaacaggac gttacgctgt tcagcagctt atttatgagc
    24301 ataccttatt ccctttatat gctccgtttg taggcaagga gcgccgagac gaagctattc
    24361 ggttaatgga gtaccaagcg caaggtgcgg tgcatttaat gctaggagtc gctgcttcta
    24421 gagttaagag cgataaccgc tttagatact gccctgattg cgttgctctt cagctaaata
    24481 ggtatgggga agccttttgg caacgagatt ggtatttgcc cgctttgcca tattgtccaa
    24541 aacacggtgc tttagtcttc tttgatagag ctgtagatga tcaccgacat caattttggg
    24601 ctttgggtca tactgagctg ctttcagact accccaaaga ctccctatct caattaacag
    24661 cactagctgc ttatatagcc cctctgttag atgctccacg agcgcaagag ctttccccaa
    24721 gccttgagca gtggacgctg ttttatcagc gcttagcgca ggatctaggg ctaaccaaaa
    24781 gcaagcacat tcgtcatgac ttggtggcgg agagagtgag gcagactttt agtgatgagg
    24841 cactagagaa actggattta aagttggcag agaacaagga cacgtgttgg ctgaaaagta
    24901 tattccgtaa gcatagaaaa gcctttagtt atttacagca tagtattgtg tggcaagcct
    24961 tattgccaaa actaacggtt atagaagcgc tacagcaggc aagtgctctt actgagcact
    25021 ctataacgac aagacctgtt agccagtctg tgcaacctaa ctctgaagat ttatctgtta
    25081 agcataaaga ctggcagcaa ctagtgcata aataccaagg aattaaggcg gcaagacagt
    25141 ctttagaggg tggggtgcta tacgcttggc tttaccgaca tgacagggat tggctagttc
    25201 actggaatca acagcatcaa caagagcgtc tggcacccgc ccctagagtt gattggaacc
    25261 aaagagatcg aattgctgta cgacaactat taagaatcat aaagcgtcta gatagtagcc
    25321 ttgatcaccc aagagcgaca tcgagctggc tgttaaagca aactcctaac ggaacctctc
    25381 ttgcaaaaaa tctacagaaa ctgcctttgg tagcgctttg cttaaagcgt tactcagaga
    25441 gtgtggaaga ttatcaaatt agacggatta gccaagcttt tattaagctt aaacaggaag
    25501 atgttgagct taggcgctgg cgattattaa gaagtgcaac gttatctaaa gagcggataa
    25561 ctgaggaagc acaaagattc ttggaaatgg tttatgggga agagtgagtg gttaggctag
    25621 ctacatttaa tgacaatgtg caggttgtac atattggtca tttattccgt aactcgggtc
    25681 ataaggagtg gcgtattttt gtttggttta atccaatgca agaacggaaa tggactcgat
    25741 ttactcattt gcctttatta agtcgagcta aggtggttaa cagtacaaca aagcaaataa
    25801 ataaggcgga tcgtgtgatt gagtttgaag catcggatct tcaacgagcc aaaataatcg
    25861 attttcctaa tctctcgtcc tttgcttccg tacgcaacaa ggatggagcg cagagttcat
    25921 ttatttacga agctgaaaca ccatatagca agactcgtta tcacatccca cagttagagc
    25981 tagctcggtc attattttta attaactcct atttctgtcg aagctgtttg agcagtaccg
    26041 ctttacagca agagttcgac gttcagtatg aggttgagcg agatcattta gagataagga
    26101 tcttacccag ttcatcgttt cctaaagggg cgttagagct gtcggccgta gtgcagcttt
    26161 tggtttggtt gttttcggat caagatgtta tggattcgta tgaaagtatt tttaggcact
    26221 atcaacaaaa tagagaaatt aagaacggcg ttgaaagctg gtgctttagc tttgaccctc
    26281 cgcccatgca gggttggaaa ttacatgtaa aaggacgttc ttctaacgag gataaggatt
    26341 atttagttga ggaaatagta ggtttagaaa tcaacgctat gcttcctagc acaacagcta
    26401 ttagccatgc ctcttttcag gaaaaggagg caggtgatgg tagtacgcag cacatagcgg
    26461 tttcaacaga gtcagttgtt gatgatgagc atctacagtt ggacgatgag gaaacagcca
    26521 atatagacac agacacacga gtcatagagg ctgagccgac atggataagt tttagtagac
    26581 ctagtcgaat tgaaaaatct cgcagggcaa gaaaaagtag ccaaactatt ttagaaaaag
    26641 aagaagcaac aacaagtgaa aatagtaatt tggttagtac tgatgagcca cacttaggtg
    26701 gtgtcctagc agcggcagat gtgggtggga agcaggatgc aaccaattac aactctattt
    26761 ttgctaatcg atttgctgct tttgatgagc tactttcaat tctaaaaact aaatttgcat
    26821 gtcgggtgct ttttgaagaa accttggttt tgccaaaagt tgggcgtagc cgattacatc
    26881 tgtgtaaaga tggctcacca agagtgatta aagccgttgg ggtgcaacgt aatggcagtg
    26941 aatttgtatt gctagaggtg gatgcatcgg atggggtgaa aatgctttct accaaagtgt
    27001 tgagtggcgt tgatagcgaa acatggcgga atgattttga aaagatacgg cgtggagtgg
    27061 tgaagagctc attgaattgg ccaaatagtt tgtttgatca attatatgga caagacgggc
    27121 atagaggggt gaatcatcca aaggggttgg gggagctgca agtatcgaga gaggatatgg
    27181 aagggtgggc tgagagagtg gttagagagc aatttacgca ttaaaggaat gactgaaaga
    27241 gcctgtaaac ccttttgtgt aagtgctttt gccggtcagt taaaggtggc catttaaacg
    27301 gtcaccaaat tcgatcataa aacggttcat ggccggcttc cagttgcgga tcggcatcgt
    27361 ccatttcttg gtcgccgcct ggatagccag gtacaccacc ttcatcgctg attcgtccgt
    27421 agggaacacc ttgagttcat attaatggaa ttttctacaa atagcctccg tggttttgag
    27481 gggggattac agacgatcca tagtagtaat ccaatgagtt cttgagcgcg gcgacatgtt
    27541 tggacgcctt ggcaaaaatt agagcctgct tgaagtgcag gcgagcacgt gcttggacga
    27601 ttgatccatt cgcggtcaaa aactcaatct tggatgacag cgtgtcagga aatccaatat
    27661 cgaagtcccg ccccgtgtca aagtagggca tttcatgatc aaaggacgaa gcttcccagg
    27721 ccacttccct aatattccag ccgctagaaa caagatttcg tacgacttca tcaacttttt
    27781 tgctttccaa cttgtcacaa agtgcatagg caagagcttt ggcggcagac acgtgcgttt
    27841 cctcttaaac ataggtcgaa cggatacccg aggcaaagcg gagcattagt cccaaagcat
    27901 ctccggtaga tccggaggct ccatactgaa ggcatggcat cttgggcatt gatatttgaa
    27961 acttctctct agatagcagg gatttcttgg ccttgcctca tgctgatagc ttgtaaatat
    28021 cacacgatcc ttagccccac aaagtgagca tactccgtag ctttcattta tagaaatcgt
    28081 cgttagattg tcgcagcatg tgcacgttcc gactatttgc tcaggaagca tcgccatacc
    28141 ttggcctagg taaagaagat cagactcgta gccacatcgg tggcaactga gcttgtacgc
    28201 aatgcccatc tggttgtctc acctgaatcg taacgtcaga gctattcatc catccattcg
    28261 gcatcaatgt atgccactaa gccgaccagc tgaccgtttg gactgaagta tgttgcctgc
    28321 accccacttg acatctcatc aataccaccg cactgcgcta ggcgccggtc ccacttgccc
    28381 acagaagcgt caggatcggc tgagttcccc aagacccttg tgagccatgc ctcctttaac
    28441 tcaacctctc gaatgattgc gtcggccagc tcctccgcgt tatctagttc gacatagtct
    28501 ggaattcgga cgtacgttct gaacacagct ggatacttac ttgggcgatt gtcatacatg
    28561 acatcaacaa tgtacccgtt tgtgtaaccg tctcttggag gttcgtatga cactagtggt
    28621 tcccctcagc ttgcgactag atgttgaggc ctaacatttt attagagagc aggctagttg
    28681 cttagataca tgatcttcag gccgttatct gtcagggcaa gcgaaaattg gccatttatg
    28741 acgaccaatg ccccgcagaa gctcccatct ttgccgccat agacgccgcg cccccctttt
    28801 ggggtgtaga acatcctttt gccagatgtg gaaaagaagt tcgttgtccc attgttggca
    28861 atgacgtagt agccggcgaa agtgcgagac ccatttgcgc tatatataag cctacgattt
    28921 ccgttgcgac tattgtcgta attggatgaa ctattatcgt agttgctctc agagttgtcg
    28981 taatttgatg gactattgtc gtaattgctt atggagttgt cgtagttgct tggagaaatg
    29041 tcgtagttgg atggggagta gtcataggga agacgagctt catccactaa aacaattggc
    29101 aggtcagcaa gtgcctgccc cgatgccatc gcaagtacga ggcttagaac caccttcaac
    29161 agatcgcgca tagtcttccc cagctctcta acgcttgagt taagccgcgc cgcgaagcgg
    29221 cgtcggcttg aacgaattgt tagacattat ttgccgacta ccttggtgat ctcgcctttc
    29281 acgtagtgaa caaattcttc caactgatct gcgcgcgagg ccaagcgatc ttcttgtcca
    29341 agataagcct gcctagcttc aagtatgacg ggctgatact gggccggcag gcgctccatt
    29401 gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct gtaccaaatg
    29461 cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg cgagttccat
    29521 agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg atcaaagagt
    29581 tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt cagcaagata
    29641 gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc attgcgctgc
    29701 cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc
    29761 acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga agccgaagtt
    29821 tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac ggtcaccgta
    29881 accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga gccgtacaaa
    29941 tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac ctctgatagt
    30001 tgagtcgata cttcggcgat caccacttcc ctcatgatgt ttaacgtttg aaataagggg
    30061 cgccgagcgc cagcgagggg agccaaaagc ttgcttttgg ccgtcccgac ttgattgaag
    30121 ggttgggcga ttttgccatt agatttttta taaatttagt gtgtttagaa tggtgatcgc
    30181 atttttcttg gcttttatgc ttgatgttaa attcgacccc aagtttcctg taagtgcgga
    30241 cacaaaaaca tatttatgtc ctgatttgct tataataaac ccttcaaacc atccgttttg
    30301 taaggttcta tttgctgtga atcctgcacc agttttccca tacagttttg tactattatc
    30361 cagatcttgt agatacatgt tctctatggt gttttctatg gctgagtttt taactgggag
    30421 attgtgatta ataattttac gcaggaattg aatttgttct tctggtgaaa tttttaagct
    30481 actttcgagc catgcttctg ttaatccgtt gtttctttct ttatctccag agaagtcttg
    30541 atttccataa tcaaaatctt tgagataatt cttgatttta tttaatccaa ttttttgggt
    30601 tatttcttgc gaaacccaaa caacagaaaa ttgcatccac gtctttggtg tatgattgct
    30661 gttccagatc tccattcctt tgggggtttt atcccatttg aatatggttt tctgatctat
    30721 tatttccgca tcaaatgcca taagtgataa tgcgatcttg aaagttgaat ctggtgccat
    30781 ttgcgttgca cactttgctt tattgaattg agcaatttca gcgtttgtgg atgcatcgta
    30841 aagtaaaaaa caaccttcag ttccttcaaa taatggagat gcaacagtag agatatctgt
    30901 tgatgcactg gcgctgctgt agataatatt tgcaattatt aaaaaaatag cgaagttgat
    30961 atgtattgtg tttttcataa taagtattgg tttggtaaag ggcttaattt taacggctaa
    31021 caattaatga ggctccgggt tcgcccaact ttgttttagg gcgactgccc tgctgcgtaa
    31081 catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct tgctgcttgg
    31141 atgcccgagg catagactgt accccaaaaa aacagtcata acaagccatg aaaaccgcca
    31201 ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac
    31261 gctacttgca ttacagctta cgaaccgaac aggcttatgt ccactgggtt cgtgccttca
    31321 tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gaggcatttc
    31381 tgtcctggct ggcgaacgag cgcaaggttt cggtctccac gcatcgtcag gcattggcgg
    31441 ccttgctgtt cttctacggc aaggtgctgt gcacggatct gccctggctt caggagatcg
    31501 gaagacctcg gccgtcgcgg cgcttgccgg tggtgctgac cccggatgaa gtggttcgca
    31561 tcctcggttt tctggaaggc gagcatcgtt tgttcgccca gcttctgtat ggaacgggca
    31621 tgcggatcag tgagggtttg caactgcggg tcaaggatct ggatttcgat cacggcacga
    31681 tcatcgtgcg ggagggcaag ggctccaagg atcgggcctt gatgttaccc gagagcttgg
    31741 cacccagcct gcgcgagcag ctgtcgcgtg cacgggcatg gtggctgaag gaccaggccg
    31801 agggccgcag cggcgttgcg cttcccgacg cccttgagcg gaagtatccg cgcgccgggc
    31861 attcctggcc gtggttctgg gtttttgcgc agcacacgca ttcgaccgat ccacggagcg
    31921 gtgtcgtgcg tcgccatcac atgtatgacc agacctttca gcgcgccttc aaacgtgccg
    31981 tagaacaagc aggcatcacg aagcccgcca caccgcacac cctccgccac tcgttcgcga
    32041 cggccttgct ccgcagcggt tacgacattc gaaccgtgca ggatctgctc ggccattccg
    32101 acgtctctac gacgatgatt tacacgcatg tgctgaaagt tggcggtgcc ggagtgcgct
    32161 caccgcttga tgcgctgccg cccctcacta gtgagaggta gggcagcgca agtcaatcct
    32221 ggcggattca ctacccctgc gcgaaggcca tcggtgccgc atcgaacggc cggttgcgga
    32281 aagtcctccc tgcgtccgct gatggccggc agcagcccgt cgttgcctga tggatccaac
    32341 ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg
    32401 acaatggagg tggtagccga gggtgtggaa acacccgact gccttgcgtg gttgcggcag
    32461 gcgggttgcg acacggtgca gggtttcctg ttcgccaggc cgatgccggc ggcggccttc
    32521 gtcggcttcg tcaaccaatg gaggaacacc accatgaacg ccaatgaacc gagcaccagt
    32581 tgctgcgtgt gctgcaagga aatcccgctc gatgccgcct tcacgccgga aggggccgag
    32641 tacgtggagc atttctgcgg gctggagtgc tatcagcgct tccaggcgcg ggccagcact
    32701 gcgaccgaaa ccagcgtcaa accggacgct tgtgattcgc cgccgtcagg ttgaggcata
    32761 ccctaacctg atgtcagatg ccatgtgtaa attgcgtcag gataggattg aattttgaat
    32821 ttattgacat atctcgttga aggtcataga gtcttccctg acattttgca gggaattcca
    32881 tgactggaca gcgcattggg tatatcaggg tcagcacctt cgaccagaac ccggaacggc
    32941 aactggaagg cgtcaaggtt gatcgcgctt ttagcgacaa ggcatccggc aaggatgtca
    33001 agcgtccgca actggaagcg ctgataagct tcgcccgcac cggcgacacc gtggtggtgc
    33061 atagcatgga tcgcctggcg cgcaatctcg atgatttgcg ccggatcgtg caaacgctga
    33121 cacaacgcgg cgtgcatatc gaattcgtca aggaacacct cagttttact ggcgaagact
    33181 ctccgatggc gaacctgatg ctctcggtga tgggcgcgtt cgccgagttc gagcgcgccc
    33241 tgatccgcga gcgtcagcgc gagggtattg cgctcgccaa gcaacgcggg gcttaccgtg
    33301 gcaggaagaa atccctgtcg tctgagcgta ttgccgaact gcgccaacgt gtcgaggctg
    33361 gcgagcaaaa gaccaagctt gctcgtgaat tcggaatcag tcgcgaaacc ctgtatcaat
    33421 acttgagaac ggatcagtaa atatgccacg tcgttccatc ctgtccgccg ccgagcggga
    33481 aagcctgctg gcgttgccgg actccaagga cgacctgatc cgacattaca cattcaacga
    33541 taccgacctc tcgatcatcc gacagcggcg cgggccagcc aatcggctgg gcttcgcggt
    33601 gcagctctgt tacctgcgct ttcccggcgt catcctgggc gtcgatgaac taccgttccc
    33661 gcccttgttg aagctggtcg ccgaccagct caaggtcggc gtcgaaagct ggaacgagta
    33721 cggccagcgg gagcagaccc ggcgcgagca cctgagcgag ctgcaaaccg tgttcggttt
    33781 ccggcccttc accatgagcc attaccggca ggccgtccag atgctgaccg agctggcgat
    33841 gcaaaccgac aaaggcatcg tgctggccag cgccttgatc gggcacctgc ggcggcagtc
    33901 ggtcattctg cccgccctca acgccgtcga gcgggcgagt gccgaggcga tcacccgtgc
    33961 taaccggcgc atctacgacg ccttggccga accactggcg gacgcgcatc gccgccgcct
    34021 cgacgatctg ctcaagcgcc gggacaacgg caagacgacc tggttggctt ggttgcgcca
    34081 gtctccggcc aagccaaatt cgcggcatat gctggaacac atcgaacgcc tcaaggcatg
    34141 gcaggcactc gatctgccta ccggcatcga gcggctggtt caccagaacc gcctgctcaa
    34201 gattgcccgc gagggcggcc agatgacacc cgccgacctg gccaaattcg agccgcaacg
    34261 gcgctacgcc actctcgtgg cgctggccac cgagggcatg gccaccgtca ccgacgaaat
    34321 catcgacctg cacgaccgca tcctgggtaa gctgtttaac gctgccaaga ataagcatca
    34381 gcagcagttc caggcgtcag gcaaggccat caacgccaag gtacgtctgt acgggcgcat
    34441 cggtcaggcg ctgatcgacg ccaagcaatc aggccgcgat gcgtttgccg ccatcgaggc
    34501 cgtcatgtcc tgggattcct ttgccgagag cgtcaccgag gcgcagaagc tcgcgcaacc
    34561 cgatgacttc gatttcctgc atcgcatcgg cgagagctac gccaccctgc gccgctatgc
    34621 accggaattc cttgccgtgc tcaagctgcg ggccgcgccc gccgccaaaa acgtgcttga
    34681 tgccattgag gtgctgcgcg gcatgaacac cgacaacgcc cgcaagctgc cagccgatgc
    34741 accgaccggc ttcatcaagc cgcgctggca gaaactggtg atgaccgacg ccggcatcga
    34801 ccggcgctac tacgaactgt gcgcgctgtc cgagttgaag aactccctgc gctcgggcga
    34861 catctgggtg cagggttcac gccagttcaa ggacttcgag gactacctgg taccgcccga
    34921 gaagttcacc agcctcaagc agtccagcga attgccgctg gccgtggcca ccgactgcga
    34981 acaatatctg catgagcggc tgacgctgct ggaagcacaa cttgccaccg tcaaccgcat
    35041 ggcggcagcc aacgacctgc cggatgccat catcaccgag tcgggcttga agatcacgcc
    35101 gctggatgcg gcggtgcccg acaccgcgca ggcgctgata gaccagacag ccatggtcct
    35161 gccgcacgtc aagatcaccg aactgctgct cgaagtcgat gagtggacgg gcttcacccg
    35221 gcacttcacg cacttgaaat cgggcgatct ggccaaggac aagaacctgt tgttgaccac
    35281 gatcctggcc gacgcgatca acctgggcct gaccaagatg gccgagtcct gccccggcac
    35341 gacctacgcg aagctcgctt ggctgcaagc ctggcatacc cgcgacgaaa cgtactcgac
    35401 agcgttggct gaactggtca acgctcagtt tcggcatccc tttgccgggc actggggcga
    35461 tggcaccaca tcatcatcgg acggacagaa tttccgaacc gctagcaagg caaagagcac
    35521 ggggcacatc aacccaaaat atggcagcag cccaggacgg actttctaca cccacatctc
    35581 cgaccaatac gcgccattcc acaccaaggt ggtcaatgtc ggcctgcgcg actcaaccta
    35641 cgtgctcgac ggcctgctgt accacgaatc cgacctgcgg atcgaggagc actacaccga
    35701 cacggcgggc ttcaccgatc acgtcttcgc cctgatgcac ctcttgggct tccgcttcgc
    35761 gccgcgcatc cgcgacctgg gcgacaccaa gctctacatc ccgaagggcg atgccgccta
    35821 tgacgcgctc aagccgatga tcggcggcac gctcaacatc aagcacgtcc gcgcccattg
    35881 ggacgaaatc ctgcggctgg ccacctcgat caagcagggc acggtgacgg cctcgctgat
    35941 gctcaggaaa ctcggcagct acccgcgcca gaacggcttg gccgtcgcgc tgcgcgagtt
    36001 gggccgcatc gagcgcacgc tgttcatcct cgactggctg caaagcgtcg agctacgccg
    36061 ccgcgtgcat gccgggctga acaagggcga ggcgcgcaat gcgctggccc gtgccgtgtt
    36121 cttcaaccgc cttggtgaaa tccgtgaccg cagtttcgag cagcagcgct accgggccag
    36181 cggcctcaac ctggtgacgg cggccatcgt gctgtggaac acggtctacc tggagcgtgc
    36241 ggcgcatgcg ttgcgcggca atggtcatgc cgtcgatgac tcgctattgc agtacctgtc
    36301 gccactcggc tgggagcaca tcaacctgac cggtgattac ctatggcgca gcagcgccaa
    36361 gatcggcgcg gggaagttca ggccgctacg gcctctgcaa ccggcttagc gtgctttatt
    36421 ttccgttttc tgagacgacc cctattatgg cgaaggtatc attcaggaag cgttgatgaa
    36481 ggattatctc tgtaaaaagt tattcaaccg gctttccggt accctggtga tcagagcgcg
    36541 ctgcggcaat aacattactg gcctggcatg ttgcaatatt ctttatccct cgccccgata
    36601 cagcggtcag ctgcatatta aagagctgta tgtttctcag tgcgatcgaa ataagggcac
    36661 gggtaaagcg ataatgcgct ttatagcccg gcttgcgctt gaacaggaat gccttagcct
    36721 tagctggaac gctgaaaaat ccaaccccgg cgctaaccgt ttttatcagg ctctgggagg
    36781 cagaataaat gatcatatcg tcaattatta cctccacggg gagagcctga gcaaactggc
    36841 ctcaggcatt tgagaagcac acggtcacac tgcttccggt agtcaataaa ccggtaaacc
    36901 agcaatagac ataagcggct atttaacgac cctgccctga accgacgacc gggtcgaatt
    36961 tgctttcgaa tttctgccat tcatccgctt attatcactt attcaggcgt agcaaccagg
    37021 cgtttaaggg caccaataac tgccttaaaa aaattacgcc ccgccctgcc actcatcgca
    37081 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg
    37141 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
    37201 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
    37261 cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc
    37321 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
    37381 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
    37441 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacggaattc
    37501 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
    37561 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
    37621 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
    37681 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
    37741 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
    37801 tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg gcccagggct tcccggtatc
    37861 aacagggaca ccaggattta tttattctgc gaagtgatct tccgtcacag gtatttattc
    37921 ggcgcaaagt gcgtc
//
LOCUS       DAJTQQ010000031        36883 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000031 DAJTQQ010000000
VERSION     DAJTQQ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36883
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.031"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..401)
                     /locus_tag="OO309_001735"
     CDS             complement(<1..401)
                     /locus_tag="OO309_001735"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5846903.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMV"
     gene            complement(487..783)
                     /gene="fis"
                     /locus_tag="OO309_001736"
     CDS             complement(487..783)
                     /gene="fis"
                     /locus_tag="OO309_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR5846904.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(809..1774)
                     /gene="dusB"
                     /locus_tag="OO309_001737"
     CDS             complement(809..1774)
                     /gene="dusB"
                     /locus_tag="OO309_001737"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR5846905.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2103..2984)
                     /gene="prmA"
                     /locus_tag="OO309_001738"
     CDS             complement(2103..2984)
                     /gene="prmA"
                     /locus_tag="OO309_001738"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR5846906.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(2996..4447)
                     /gene="panF"
                     /locus_tag="OO309_001739"
     CDS             complement(2996..4447)
                     /gene="panF"
                     /locus_tag="OO309_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR5846907.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(4437..4679)
                     /locus_tag="OO309_001740"
     CDS             complement(4437..4679)
                     /locus_tag="OO309_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR5846908.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(4788..6137)
                     /gene="accC"
                     /locus_tag="OO309_001741"
     CDS             complement(4788..6137)
                     /gene="accC"
                     /locus_tag="OO309_001741"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR5846909.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6148..6618)
                     /gene="accB"
                     /locus_tag="OO309_001742"
                     /gene_synonym="fabE"
     CDS             complement(6148..6618)
                     /gene="accB"
                     /locus_tag="OO309_001742"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR5846910.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(7596..8570)
                     /locus_tag="OO309_001743"
     CDS             complement(7596..8570)
                     /locus_tag="OO309_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5846911.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            8722..10662
                     /gene="csrD"
                     /locus_tag="OO309_001744"
     CDS             8722..10662
                     /gene="csrD"
                     /locus_tag="OO309_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR5846912.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            10967..12010
                     /gene="mreB"
                     /locus_tag="OO309_001745"
     CDS             10967..12010
                     /gene="mreB"
                     /locus_tag="OO309_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR5846913.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12076..13179
                     /gene="mreC"
                     /locus_tag="OO309_001746"
     CDS             12076..13179
                     /gene="mreC"
                     /locus_tag="OO309_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR5846914.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13179..13667
                     /gene="mreD"
                     /locus_tag="OO309_001747"
     CDS             13179..13667
                     /gene="mreD"
                     /locus_tag="OO309_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR5846915.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            13676..14269
                     /gene="yhdE"
                     /locus_tag="OO309_001748"
     CDS             13676..14269
                     /gene="yhdE"
                     /locus_tag="OO309_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR5846916.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            14259..15728
                     /gene="rng"
                     /locus_tag="OO309_001749"
     CDS             14259..15728
                     /gene="rng"
                     /locus_tag="OO309_001749"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR5846917.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            15796..19596
                     /gene="yhdP"
                     /locus_tag="OO309_001750"
     CDS             15796..19596
                     /gene="yhdP"
                     /locus_tag="OO309_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR5846918.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            19752..21197
                     /gene="tldD"
                     /locus_tag="OO309_001751"
     CDS             19752..21197
                     /gene="tldD"
                     /locus_tag="OO309_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR5846919.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(21325..22254)
                     /gene="aaeR"
                     /locus_tag="OO309_001752"
     CDS             complement(21325..22254)
                     /gene="aaeR"
                     /locus_tag="OO309_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR5846920.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            22437..22640
                     /locus_tag="OO309_001753"
     CDS             22437..22640
                     /locus_tag="OO309_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR5846921.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            22648..23580
                     /gene="aaeA"
                     /locus_tag="OO309_001754"
     CDS             22648..23580
                     /gene="aaeA"
                     /locus_tag="OO309_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR5846922.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            23586..25553
                     /gene="aaeB"
                     /locus_tag="OO309_001755"
     CDS             23586..25553
                     /gene="aaeB"
                     /locus_tag="OO309_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR5846923.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            25645..25917
                     /locus_tag="OO309_001756"
     CDS             25645..25917
                     /locus_tag="OO309_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR5846924.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(25973..26236)
                     /gene="yhcN"
                     /locus_tag="OO309_001757"
     CDS             complement(25973..26236)
                     /gene="yhcN"
                     /locus_tag="OO309_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR5846925.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(26601..27071)
                     /gene="argR"
                     /locus_tag="OO309_001758"
     CDS             complement(26601..27071)
                     /gene="argR"
                     /locus_tag="OO309_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR5846926.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            27506..28444
                     /gene="mdh"
                     /locus_tag="OO309_001759"
     CDS             27506..28444
                     /gene="mdh"
                     /locus_tag="OO309_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5846927.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(28506..29573)
                     /gene="degS"
                     /locus_tag="OO309_001760"
     CDS             complement(28506..29573)
                     /gene="degS"
                     /locus_tag="OO309_001760"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR5846928.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(29663..31010)
                     /gene="degQ"
                     /locus_tag="OO309_001761"
                     /pseudo
     CDS             complement(29663..31010)
                     /gene="degQ"
                     /locus_tag="OO309_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31164..31562)
                     /locus_tag="OO309_001762"
     CDS             complement(31164..31562)
                     /locus_tag="OO309_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR5846929.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            31756..32883
                     /gene="zapE"
                     /locus_tag="OO309_001763"
     CDS             31756..32883
                     /gene="zapE"
                     /locus_tag="OO309_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR5846930.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33102..33530
                     /gene="rplM"
                     /locus_tag="OO309_001764"
     CDS             33102..33530
                     /gene="rplM"
                     /locus_tag="OO309_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR5846931.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            33546..33938
                     /gene="rpsI"
                     /locus_tag="OO309_001765"
     CDS             33546..33938
                     /gene="rpsI"
                     /locus_tag="OO309_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR5846932.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            34333..34971
                     /gene="sspA"
                     /locus_tag="OO309_001766"
     CDS             34333..34971
                     /gene="sspA"
                     /locus_tag="OO309_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR5846933.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            34977..35474
                     /gene="sspB"
                     /locus_tag="OO309_001767"
     CDS             34977..35474
                     /gene="sspB"
                     /locus_tag="OO309_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR5846934.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(35517..>36883)
                     /gene="dcuC"
                     /locus_tag="OO309_001768"
     CDS             complement(35517..>36883)
                     /gene="dcuC"
                     /locus_tag="OO309_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5846935.1"
                     /translation="FGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGFG
                     GVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSLL
                     SRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAVA
                     VIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRAF
                     DKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVVM
                     LLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIGT
                     VDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVPA
                     VVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIATI
                     TLFY"
BASE COUNT         8931 a         9245 c         9730 g         8977 t
ORIGIN      
        1 accatcatca ggatgggttc gtacatggag ccgtagtgtt ttttcgcctg tactccagaa
       61 ctgtcatatg accagacgat gcgacttttg atggtaaaaa gcttgcggca ctggagatcg
      121 ataaagggca tgttttccgt actgttcata atgtacatgc tgccctgctt tttcagaacg
      181 cggtggcact ctgcaatcac ttcaaacagc cagtcgataa acagatcttc tttccaggct
      241 tcgatcagac catcaaaatt tttaccgatg ttatacggtg ggtcggcaaa gagcagatcg
      301 acgctttcag taggtagctt tttaagttcg gcaaaggcat caccgtgaat aatggtctta
      361 gcttcattac caaaccgggt cggttcacat cctgttctca tggtcactcc ctttgtgaca
      421 cctataaaaa aggcgcttcc ccatgccgag tagcgccttt ttaatcaagc atttagctaa
      481 cctgaattag ttcatgccgt attttttcaa ttttttacgc agcgtaccac ggttgatgcc
      541 catcatcagc gcagcacggg tctggttacc acgggtgtat tgcatcacca tgtccaacag
      601 gggctgttct acttcagcca gtaccagctc atagaggtca ttcacatcct gaccattcag
      661 ttgagcaaaa tagttcttca gtgcctgttt aaccgagtca cgcaggggtt tttgggttac
      721 ctgatcctga gagttaacgg tagaaacggt cagtacgtca gaatttacgc gttgttcgaa
      781 catagttctg tcagctcttt atttctgttt acgcaaaatt ttcgaagtat gcctccaacg
      841 cctccagctg ttcgctggca tcctcaatgg cgttgaatgt gcgccgaaac tggtcatttg
      901 gagcgtgttc ctggagatac caggaaacgt gtttacgtgc aattcggtac ccttttgccg
      961 gaccataaaa gtcatgcagt tcccgaacgt gcgcgcaaag caagcgctta acctctgcca
     1021 aaggcagtgg gggcagcaac tccccagtgt ccagataatg ctggatttcc cgaaagatcc
     1081 agggtcttcc ctgagctgcg cggcctatca tcagggcatc cgcccctgta tagtcgagca
     1141 cagctctggc tttaagcggg tcagtaatgt cgccattcgc gataaccgga atggaaactt
     1201 tctgcttaac tgcccgaata ctgtcgtact cagcttctcc attgaacaaa caggcgcgtg
     1261 tacggccatg aatggtcaga gcctgaatgc cacagtcttc agccagttgg gcaatctctt
     1321 cgcagttacg gtgttccggt gcccagccgg tgcgaatctt cagggtaaca ggaacgtcca
     1381 ctgcattgac gacctcggta aggatcgatt taacgacatc cgggtactgc aagagggctg
     1441 aacctgcgag cttgcgattc acttttttag ccgggcaacc catattgata tcaataatct
     1501 gggcaccgct ttccacgtta atacgtgctg catctgccat ttctttcgga gcgctaccag
     1561 caatttgcac ggtgcgaata ccgggttcat caatgtgcac catccgtaaa cgagatttgt
     1621 cgctttccca aacctgtggg ttagaagaca tcatctcgga tactgtcaat ccggctccca
     1681 tctcgtagca caacgtccga aaaggtctgt ctgtaatgcc agccatgggc gctgcgatca
     1741 ggcgatttct gagctgatat tgtccgatgc gcatgagtta agaaatgacc atactgtgac
     1801 tgcaaggcgg cgtatattac gcattttttg cacgagatga aaggccaaac tttgaccaat
     1861 cctctgagat ggatcaaaga attgcattta aaatgagcgt ggtgcgataa ttactcataa
     1921 aaatcatcat attagaaaat agtgactaaa atttacactc aaagaaattt gagtaagttc
     1981 tcaatttttc tttatgaatg aaaattttgg cacgcaaatt tgcgtaaata atcggcaaat
     2041 ttacgtgcct tttgtgagct tgctcgcact tcgccccgcg tcaccctacg gcgatgcgaa
     2101 ggtcaattct tacgaccggt aatacggcac cactcttctt tttccacgac cgggtccagt
     2161 gcgaagctat cggcataagc ttcacaaacg ctctctgcct ggcttgccag aataccggaa
     2221 aggcccagca aaccgcctga aactggcagg acgctgatta acggtgccag ttcacgtaat
     2281 gggcctgcaa ggatgttagc gaccaccacg tcggctttca tttcttctgg ctgatctttc
     2341 ggtaagtaga gttccagacg gtcagaaacg ccattacgtt cggcgttatc gcggctggcc
     2401 tgaatcgcct gcggatcgat atcaatacca atggcttttg ctgcacccag tttcagcgcc
     2461 gcgatcgcca gaatgccgga accacagcca aagtcgatga ctgttttacc ggttaaatcg
     2521 aggctgtcga gccattgcag gcacagagag gtggttggat gggtacccgt accaaacgcc
     2581 agccctggat ctaacatcac gttgacggcg ttttcgtccg gcacatcacg ccagctaggg
     2641 cagatccaca gtcgttcacc aaagcgcatc gggtggaaat tatccatcca ttcgcgctcc
     2701 cagtctttat cttctagttg ttcgatttta tgcgcgaagc ctgcgccgag cagcggatgg
     2761 ttttccagaa tcgccaccac gtcgttcata tcggtttcag cgtcgaacag accaatcaca
     2821 tcggtgtcgc cccacaggcg cgtttcgccc ggcagcggct caaatactgg cgtatcgtgg
     2881 gtatcctgaa aagtgataga aacggcaccc gcttccatca gcgcatcgct aagatcttcc
     2941 gcgttcgcgc cggtggtgtt cagtttcagt tggatccaag gcatggcaaa actctttatt
     3001 tatcagtagt caaaacggta gcttgcggga cggatgtacc gaaacggttt ccgaccagga
     3061 aagccagcaa acttagtagt aacgagggca cgatagggtg gaagcccagg tactgaatat
     3121 tcagcgtcgc gagtacggca tacagcacgc cgccaacgat catcgcactt agcgcgcctt
     3181 tggcgttggc gcgttcccag taaagaccca gcaccagcgg ccacaggaaa acggcttcca
     3241 gcccaccgaa ggccagcaaa ttcagccaga tgatcatttc tggcggcttc caggcggcaa
     3301 gcagcagcaa cgcgccgaga actaacgtaa ttaccgccga catccgcttc agacgcgtct
     3361 cgttttgcat ttgatccgga cggatattca gatagagatc tttaatgatc gtagcggaac
     3421 tttgcagcag ttgggcgtta attgtcgaca tgatcgcagc cataggtgca gccagaaaga
     3481 tcccggcagc aaaaggtggc agcactttta ccattaacgt cgggatcacc agatctggta
     3541 cggtgagatc ggggatcact gcacgaccta acgctccggc caggtgcata ccgaacatca
     3601 ggattgcgac tataatcgta ccgatgatga tcccccggtg tactgctttg ctgtctttat
     3661 aagagataca gcgcaccgca gtatgcggca ggccaatcac gccaaagcac accagtaccc
     3721 agaacgacgt cataaaggca ggcgacagaa tatcgtcagc gccttgtggc gtaaccagtt
     3781 gcggatcgat ggtttgcaag gtctgtactg cgttgcttaa gccgccagcg gcatgtacca
     3841 cgccaataag cagcacaacg gtgccaatca gcatcacaag cccttgcatg gtgtcgttca
     3901 gcacgctggc gcgaaagcca ccaaaggcgg tatataacgc aatgctgata ccaaaaatca
     3961 gcagcccggt ttcataagga atacccgccg cggtttccag caggcgcgca ccgccgataa
     4021 actgcacggt catcgcacca acgaacgcaa ccagcaaact caaactcgcc agccacacca
     4081 gaagacgact ctggtagcgg gcaaacagca tatcgttcag cgtcactgca ttgtagcggc
     4141 gcgcaagaat cgcaaacttc ttgccgagaa taccgagtga aagccagaca gcaggaagct
     4201 gaatcatcgc cagcaatacc cagcccagcc cgtatttata agcagctcct ggcccgccga
     4261 taaacgaact ggcactgata taggtcgcgg tgagagtcat cgccagcaca ataccgccca
     4321 tagagcggct gccgaggaaa tactcattaa ggaaggtgcc ggtgctccgt ttacgcatcg
     4381 cataaaccga gataccgaac accaccacca gataggcgac cagcggtaga attacttcaa
     4441 gctgcatcgt catcctccag tgggatatcg cgatagataa atttcaccat cgcccagcac
     4501 agtccaataa acagtagcgg cgtcaggatg caggccatct caaaccagcg cggaaagccg
     4561 gtaaaaccgg gggcaacgcc aggtaagtaa gcgtctacta accaaactgc cagatacaaa
     4621 agggtcagcc ccagcgccca gcgggcctct ttatgggcct gaacaaaacg agtgtccatt
     4681 ttttgtccct gatgggtgaa gaaagcgagg attgtacctt atgggggttg tcgatcccca
     4741 gtaataaaaa aggccggaaa atccggcctt ttgacgcttt tgcagtctta tttttcctga
     4801 agaccgagtt ttttctccag atagtggatg ttagtgccac catgctggaa gttctcgtca
     4861 ttcatgatgc ggatctgcag atcaacgttg gttttgatac cgtcgatgat cagctcctgc
     4921 agcgcattct tcatgcgggc aatcgccacg tcacggtttt caccgtagca aatcagctta
     4981 ccgatcattg agtcatagta cggcggtacg gtgtagcccg cgtagatatg agactcccaa
     5041 cgtacgccaa aaccgccagg tgcgtggaaa cgggtgattt tgcctgggct tggcaggaag
     5101 gtgttcggat cttcggcgtt gatacgacat tccaccgcat ggccgcgaac gtgaacttct
     5161 tcttgcttga tcgacagcgg ttgaccggca gcgatacgca gctgttcttt gatcaggtca
     5221 acgccggtga tcatttctgt aaccgggtgt tctacctgaa tacgggtgtt catttcgatg
     5281 aaatagaact cgccgttttc gaacaggaac tcgaaagtac ctgcaccgcg atagccgata
     5341 tcaacacacg ctttagcgca acgttcgccg atgtagcgac gcagttccgg ggtaatgccc
     5401 ggtgctggtg cttcttcgac cactttctgg tggcggcgct gcatggagca gtcacgttcc
     5461 gccagataga tagcgttgcc ctgaccgtca gccagtacct gaatctcgac gtggcgagga
     5521 ttctccaggt atttctccat gtaaaccata tcgttgctga aagcagcttt cgcttccgca
     5581 cgggtcatgg agatggattg tgccagttca gcgtcgccgc gcactacgcg cataccgcga
     5641 ccgccgccgc cgccggaggc tttgataatc accggataac caatgcgttt agcaatggcg
     5701 cggtttttat ccatatcgtc gcccagcggg ccgtcagaac caggtacgca aggaacgccc
     5761 gcttttttca tcgccgcgat tgcggatact ttgtcgccca tcaggcgaat ggtttctgct
     5821 ttcgggccga tgaagataaa gccggagcgt tcaacctgct cggcaaagtt ggcgttctcg
     5881 gagaggaagc cgtaacccgg atggattgct actgcgccgg tgatttcagc ggcgctgatg
     5941 attgccggga tgttcagata actttttact gacggagcag ggccaataca gaccgtttca
     6001 tctgccagta atacgtgttt tagatcgcga tccgtgctgg agtgcacagc gacagtcttg
     6061 atgcccagtt ctttacaggc acgaagaata cgcaatgcaa tctcgccgcg gttggcaata
     6121 acaattttat ccagcatgtt cgcctcgtta ctcgatgacg accagcggct cgtcaaattc
     6181 taccggttgt ccactttcga ccagaattgc tttcacggta ccggatttgt ccgcttcgat
     6241 ctggttcatc attttcatgg cttcaacgat gcacagggta tcgcccacgt tgactttctg
     6301 acccacttcg atgaacgctt ttgcgtccgg gcttggggtg cggtagaaag taccaaccat
     6361 cggggaacgt acgatgtgac cactgatttc cgctgctgct ggcgcttcca tggaaggaac
     6421 ggtcgccgga gcggctgcgt tagattgagc tggctgctgc atcattggtg cagcgtaagc
     6481 ttgttgcatc acagggaaac ttgcggcagg agctgcacgg ctaatgcgta ctgactcttc
     6541 gccttcagaa atttccagtt cggagatgcc tgattcttca accagctcga tcagtttttt
     6601 aatcttacga atatccatga gtgggttccg tactctttgt ttagtgtgat tgtgacaggc
     6661 gtttcaccgc cgtctgtaaa gcgtatgccc cgacagctgt atgcatagcg ataaattcca
     6721 gcaggccggg acgccggtct attttgcccc agagtgtcaa tgttagactt gacggacatt
     6781 gtgcagcgat ctgctgctat cctccggcaa aaaacaaaat ataccttcgt tgcgccaatg
     6841 tcaccttttc cgcagcgcaa aaactgcgtc agggaggacg aggtcgcaca ttataacgat
     6901 ttcgtagcaa ttggcagcta aatactggtc ttatcaggga atataatcaa cagctaacat
     6961 gtaaataacc ttcaacaccg tgtaatttgc aacaagccgc acaattcacg aaattagcgc
     7021 catcatcgac ggaacttctt ataacgtaag gtgaaaagct gaaaagccag ccctgcgaag
     7081 atgaggggct gcaatgagat aatcttcaca gaccacaaat aatgtaggga tgccaggatc
     7141 gcgacaagat agacgacatt acgcagcggt tgctggggat tacccaattt tcactgcatc
     7201 gtctggatag atgtgaatgc taaagcacgc aaaatcaacc aactgacaat tctcagcatt
     7261 aaataggttc gagtggttaa tttgtagtca aataaaatca gattattcac cccatggtac
     7321 cagaaatcag taacaggtta agcgtagtgt cgcccaggca aagcaccata aacctaacag
     7381 gcgacggagg cgtatcaata atggctgttt agaggagtat caatgcgcat caatttgtgg
     7441 tcagtcgaaa tcatgacttt ccagtttgga tgatgttgta caacttcctg accaaggtgt
     7501 tcgacgaaag ctgtccggct aaccgctttt cccgttaacc gattgaataa tgatgtcaaa
     7561 atgagttccc tctcttatta ttccttgcta aatggttagt taaccttcac cagcgtgcga
     7621 ccctggatct ggttattaat gatggcctcg gcaaagttcg gtgcctctga cagagatatc
     7681 tcttttgccg cctgggtata gaatgattcc ggtaaatcgg cgaccagtcg ctgccaggct
     7741 tgtgcgcggc gttctggtgg cgtcattact gaatccaccc cttgcaaacg gacattacgc
     7801 agaataaatg gcatgaccgt ggttggcaga gtaaaaccac ccgccagacc acaggccgcc
     7861 acgcagccgc cgtaattcat ttgcgccagc acttttgcca gcactttgtc gccaacagtg
     7921 tcaattgccc cagcccagac ttgtttttcc agaggacggg attcggcaaa ctcatcacga
     7981 gggagaatac cgctggcacc taaacttttc agatattcat gggtactttc gcgaccggaa
     8041 acggcaacga cctgataacc caacttatgc agcagcgcca cggcggtact gccgacgcca
     8101 ccactggcac ccgtcacgac aatctcaccg tcctgcgggc gaacaccggc atcttccagc
     8161 gccatcacac acagcatggc ggtaaaaccg gcagtaccga taatcattgc tttacgcgcg
     8221 tccagccctt gcggcatggc aaccagccag tcacctttca ctcgcgcctg ctccgccagc
     8281 ccaccccagt ggttttcacc aacgccccag ccagtgagta acacctcctg accggcatga
     8341 aaacgcggat cttcgctggt gcgtacagtt ccggcaaaat cgatcccagg aatcatcgga
     8401 aaattacgga tgatttttcc cttaccggta atcgccagcg catctttata gttcaggctc
     8461 gaccagtgaa catcgaccgt gacatcgccc tccggcaggc gacgttcgtc cagagtctgt
     8521 actgatgcga gagttttgcc gtcctgctgt tctaaaagta acgcctgcat aagtggtcct
     8581 catgtgcatg atggattgga aaataatttc tgaagactat actcgctaat ggaaaagcaa
     8641 agccgatgaa gcgcaataaa ttgcgagata aatctgattt gctagtatgc ccgcttcctc
     8701 actatcggag ttaacacaag gatgagatta acgacgaaat tttcggcctt tgttacgctg
     8761 ctcaccgggt taacaatttt tgtgactttg ctgggctgtt cgctaagttt ctacaacgcc
     8821 attcagtata agtttagtca tcgcgttcag gcggtggcga cggcgattga tacccacctt
     8881 gtgtcgaatg acttcagcgc attaaggcca caaattaccg aattaatggt gtcagcagat
     8941 atcgttcgtg tagacctgct gcatggtgat aaacaggttt ataccctggc cagaaatggt
     9001 agttatcgtc cggttggcac cagcgatctg tttcgtgaac tgagcgttcc gttgataaag
     9061 catccgggga tgtcgttgcg tctggtttat caggatccga tgggcaacta tttccattcg
     9121 ttgatgacca ccgcgccgct cacgggggcg attggcttta tcattcttat gctctttctg
     9181 gcggtacgct ggttacaacg gcaacttgcc gggcaagaat tgctggaaac ccgggctact
     9241 cgtatcttaa acggtgagcg tggctctaat gtgttgggaa ccatctatga atggccgccc
     9301 agaaccagca gtgcgctgga tacgctgctt cgtgaaattc agaacgcacg cgaacaacac
     9361 agccgtcttg atacgctgat ccgctcttat gccgcacagg acgtgaaaac cggcctcaat
     9421 aaccgactct ttttcgataa tcagttagca acgttactgg aagatcagga gaaagtaggt
     9481 acccacggga tcgtgatgat gattcgtctg ccggatttca atatgttgag cgatacctgg
     9541 gggcacagcc aggttgaaga acagttcttc actctgacga atctgctgtc gacatttatg
     9601 atgcgctacc ctggcgcact gctggcgcgt taccaccgca gtgattttgc tgcgctgtta
     9661 ccgcaccgga cgttaaaaga ggcagagagc atagccagtc agttaatcaa agccgtcgat
     9721 accttgccga acaataaaat gctcgatcgc gacgatatga tccacattgg tatctgtgcc
     9781 tggcgtagtg gtcaggatac cgagcaggta atggaacatg cagagtctgc cacgcgtaat
     9841 gcgggattgc agggtggcaa tagctgggct atttatgatg actcgttgcc tgaaaaagga
     9901 cgcggtaatg ttcgctggcg tacgcttatc gagcaaatgc tcagtcgcgg cggcccgcgc
     9961 ctttatcaaa aaccggcggt tactcgcgaa ggtcaggttc atcatcgcga actcatgtgc
    10021 cgcatcttcg atggtaatga agaggttagc tcggcggagt atatgccgat ggtcttgcag
    10081 tttggcttat cggaagagta tgaccgtctg caaatcagcc gtcttattcc actattgcgt
    10141 tactggccag aggaaaatct ggcgattcag gttaccgttg agtcgctgat tcgtccgcgt
    10201 tttcagcgtt ggctgcgcga tacgttaatg caatgtgaaa aatcacaacg aaaacgcata
    10261 attattgaac ttgcagaggc cgatgtaggt caacatatca gtcgtttaca acctgttatt
    10321 cgtttagtga atgctttagg ggtacgggta gccgtcaacc aggctggttt gacgctggta
    10381 agcaccagtt ggatcaaaga acttaatgtt gagttactca agctccatcc ggggctggtc
    10441 aggaacattg agaagcgaac ggagaaccag ctgctggttc aaagcctggt ggaagcctgc
    10501 tccgggacca gcacccacgt ttacgccacc ggcgtgcgtt cgcgaagcga gtggcagacc
    10561 ctgattcagc gcggtgttac aggcgggcaa ggggattttt tcgcgtcctc acagccactt
    10621 gatactaacg tgaaaaaata ttcacaaaga tactcggttt aacctgccgt ttaatccgtt
    10681 ttcacgtaga ataacgcgcg ctgcgtctca tgggagtgtg cttgtctgct cgccagattg
    10741 ttgcagcaca tatgcagatg aatgacctta cgcggttgca aacaggcgag gaatgctgct
    10801 gatgcattaa gcctttctgg actcaggcag agatttgtaa caaaggaaac gaactgcact
    10861 aattttcacc gtagcagatg atttttgcgc cttgtcgctg ctgcgtgtgg ttggtaaagt
    10921 aagcggattt tcttttccgc cccagctttc aggattatcc cttagtatgt tgaaaaaatt
    10981 tcgtggcatg ttttccaatg acttgtccat tgacctgggt actgcgaata ccctcattta
    11041 tgtaaaagga caaggcatcg tattgaatga gccttccgtg gtggccattc gtcaggatcg
    11101 tgccggttca ccgaaaagcg tagctgcagt aggtcatgac gcgaagcaga tgctgggccg
    11161 tacgccgggc aatattgctg ccattcgccc aatgaaagac ggcgttatcg ccgacttctt
    11221 cgtgactgaa aaaatgctcc agcacttcat caaacaagtg cacagcaaca gctttatgcg
    11281 tccaagcccg cgcgttctgg tttgtgtacc ggttggcgcg acccaggttg aacgccgcgc
    11341 aattcgtgaa tccgcgcagg gcgctggtgc ccgtgaagtc ttcctgattg aagaaccgat
    11401 ggctgccgca attggtgctg gcctgccggt ttctgaagcg accggttcta tggtggttga
    11461 tatcggtggt ggtaccactg aagttgctgt tatctccttg aacggcgtgg tttactcctc
    11521 ttccgtgcgc attggtggtg atcgtttcga cgaagctatc atcaactatg tgcgtcgtaa
    11581 ttacggttct ctgatcggtg aagccaccgc agaacgtatc aagcacgaaa tcggttcggc
    11641 ttacccgggc gatgaagtcc gtgaaatcga agttcgtggc cgtaacctgg cagaaggtgt
    11701 tccacgcggt tttaccctga actccaacga aatcctcgaa gcactgcagg aaccgctgac
    11761 cggtattgtg agcgcggtaa tggttgcact ggaacagtgc ccgccggaac tggcttccga
    11821 catctccgag cgcggcatgg tgctcaccgg tggtggcgca ctgctgcgta accttgaccg
    11881 tttgttaatg gaagaaaccg gcattccagt cgttgttgct gaagacccgc tgacctgtgt
    11941 ggcgcgcggt ggcggcaaag cgctggaaat gatcgacatg cacggcggcg acctgttcag
    12001 cgaagagtaa tcggatgcag gcaggggaag cgtctgttta ccctgcctgg tctgatacga
    12061 gaatacgcat aacttatgaa gccaattttt agccgtggcc cgtcgctaca gattcgcctt
    12121 attctggcgg tgctggtggc gctcggcatt attattgccg acagccgcct ggggacgttc
    12181 agtcaaatcc gtacttatat ggataccgcc gtcagtcctt tctactttgt ttccaatgct
    12241 cctcgtgaat tgctggatgg catatcgcag acgctggcct cgcgtgacca actagaactt
    12301 gaaaaccggg cgttacgtca ggaactgttg ctgaaaaaca gtgaactgct gatgcttgga
    12361 caatacaaac aggagaacgc gcgtctgcgc gagctgctgg gttccccgct gcgtcaggat
    12421 gagcagaaaa tggtgactca ggttatctcc acggttaacg atccttatag cgatcaagtt
    12481 gttatcgata aaggtagcgt taatggcgtt tatgaaggcc agccggtcat cagcgacaaa
    12541 ggtgttgttg gtcaggtggt ggccgtcgct aaactgacca gtcgcgtgct gctgatttgt
    12601 gatgcgaccc acgcgctgcc aatccaggtg ctgcgcaacg atatccgcgt aattgcagcc
    12661 ggtaacggtt gtacggatga tttgcagctt gagcatctgc cagcgaatac ggatattcgt
    12721 gttggtgatg tgctggtgac ctccggtctg ggcggtcgtt tcccggaagg ctatccggtc
    12781 gcggttgtct cttccgtaaa actcgatacc cagcgcgctt atactgtgat ccaggcgcgt
    12841 ccgactgcag gactgcaacg tttgcgttat ctgctgctgc tgtggggcgc ggatcgtaac
    12901 ggcgctaacc cgatgacgcc ggaagaggtg catcgtgttg ctaatgaacg tctgatgcag
    12961 atgatgccgc aggtattgcc ttcgccagac gcgatggggc caaagttacc tgaaccggca
    13021 acggggatcg ctcagccgac tccgcagcaa ccggcgacag gaaatgcagc tactgcgcct
    13081 gttgcgccga cacagcccgc tgctaatcgc tctccacaaa gggctacgcc gccgcaaagt
    13141 ggtgctcaac cgcctgcgcg tgcgccggga gggcaatagt ggcgagctat cgtagccagg
    13201 gacgctgggt aatctggctc tctttcctca ttgcgctgtt gctgcaaatc atgccctggc
    13261 cggataacct gattgttttc cggccaaact gggtgttact tatcttgttg tattggatcc
    13321 tggccttgcc ccatcgcgta aatgtgggca caggttttgt gatgggtgcc atactggatc
    13381 tgatcagcgg ctcgatgctt ggcgtacgcg tattggcgat gagcatcatt gcttacctgg
    13441 tggcgctgaa ataccagctt ttccgcaacc tcgcattatg gcagcaggcg ctggtcgtca
    13501 tgttgctttc gctggtggtg gatattattg ttttctgggc agagttttta gtgattaacg
    13561 tctctttcag accggaagtg ttctggagta gtgtagtcaa tggggtgctc tggccgtgga
    13621 ttttcttgct gatgcgtaaa gtccgtcagc agtttgcagt gcaataaagg tttctatgac
    13681 ttctctgtat ttagcttccg gttctccgcg tcgtcaggag ttacttgcgc aacttggcgt
    13741 gacctttgaa cgtattgtta cgggcattga ggagcagcgt cagccgcagg agagcgcgca
    13801 gcagtatgtt gtgcgtctgg cacgcgagaa agcacaggcg ggtgtcgcgc aaacggcaca
    13861 ggatctcccg gtgctgggtg cggatattat cgttatcctg aacggagaag tgctcgagaa
    13921 accgcgcgac gcagagcatg cggcgcagat gttgcgcaaa ttatcgggtc agacccatca
    13981 ggtgatgacg gcagtggcgt tggccgacag ccagcacatt ctcgattgcc tggtggtcac
    14041 cgatgtgact ttcagaacgt taacagacga agacatcgcg ggctatgtcg ccagcggtga
    14101 accgttagat aaagcaggtg catacggtat tcaggggctg ggtggctgtt ttgtcaggaa
    14161 gataaatggc agctatcacg ccgtagtcgg cttaccgctg gttgaaacgt atgaattatt
    14221 aagtaatttt aacgcactgc gtgagaaaag ggataaacat gacggctgaa ttgttagtaa
    14281 acgtaacgcc ttcggaaacg cgagtggcgt atattgatgg cggtattctg caggaaattc
    14341 atattgaacg tgaggcgcga cgcggaatag taggcaatat ctacaagggt cgtgtaagtc
    14401 gtgtacttcc gggtatgcag gcggcttttg tagatattgg gctggataaa gccgcgtttc
    14461 ttcatgcatc cgacatcatg ccgcataccg aatgtgtggc gggtgaagaa caaaagcaat
    14521 tcacggtgcg cgacatctcg gaactggttc gtcaggggca agatctgatg gtgcaggtgg
    14581 tgaaagatcc gcttggcact aaaggtgcgc gcctgaccac cgatatcacg ctgccttctc
    14641 gctatctggt atttatgcca ggggcttctc acgttggggt ttcccaacga attgaaagcg
    14701 aatcagaacg tgaacgtctg aaaaaagtgg tcgcagagta ttgcgacgag cagggcgggt
    14761 ttatcatccg caccgcagcg gaaggggttg gcgaggctga actggcctcc gatgccgctt
    14821 atctgaaacg cgtctggacc aaagttatgg agcgtaaaaa gcgcccgcag acccgttatc
    14881 agctgtacgg cgaactggcg ctggcgcagc gtgttctgcg tgattttgcc gacgccgaac
    14941 tggaccgcat tcgcgttgac tcacgcctga cttacgaagc attgctggag tttacctcgg
    15001 agtacattcc cgagatgacc agcaagctgg agcattacac cggacgccag ccgattttcg
    15061 atctctttga tgtcgaaaac gaaatccagc gagcgttgga acgcaaagta gaactgaaat
    15121 ccggtggtta tctgattatc gaccagaccg aagcgatgac caccgtggac atcaataccg
    15181 gagcgtttgt cggtcatcgc aatctggacg acactatttt caataccaat attgaagcga
    15241 cgcaggctat cgctcgccag ttacggttgc gtaatctggg cgggattatc attattgatt
    15301 tcatcgatat gaataatgaa gatcaccgcc gccgcgtgct gcattcgctg gagcaggcgt
    15361 tgagcaaaga ccgggtgaaa accagcgtta atggtttttc ggcgctgggg ctggtggaga
    15421 tgacgcgtaa acgcacccgc gaaagcattg agcacgtact gtgtaacgaa tgcccaacct
    15481 gccacggtcg cggaacggtg aaaaccgtgg aaacggtatg ctatgaaatc atgcgcgaga
    15541 ttgttcgtgt ccaccatgct tacgactccg accgtttcct ggtctatgct tctccggcag
    15601 tagccgaagc cttgaaaggc gaagagtcac actcgctggc ggaagtggaa attttcgttg
    15661 gcaaacaggt taaagtacaa attgaaccgc tctataacca ggagcagttt gacgtcgtaa
    15721 tgatgtaaac agatgctggg ccgccatccg gcaaagggtt tttgagtcac atttttagca
    15781 gacaaggagt gacgggtgag gcgattgccg gggattttac tgcttactgg agccgcgctc
    15841 gttgtgatcg ctgccctgct ggttagcggc ctgcgtattg ctttaccgca tcttaacgcc
    15901 tggcgtccgg aaatcctcaa caaaatagaa tccgcgactg gcatgccggt agaagccagt
    15961 cagctctcag ccagctggca gaattttggc ccgacgcttg aagcacacga catccgtgca
    16021 gaactaaaag atggcggcga attttcggtt aaacgcgtta ctctggcgct ggatgtctgg
    16081 cagagcctgt tacatatgcg ctggcagttt cgcgacctca ctttctggca gctgcgcttt
    16141 cgcaccaaca ctcctatcac cagcggtggt agcgatgaca gtctggaagc cagtcacatc
    16201 agcgatctgt ttcttcgtca atttgaccat ttcgatctcc gcgacagtga agtcagtttt
    16261 ctgacgccat ccggtcagcg cgccgagctg gcgatcccac aactcacctg gctgaacgat
    16321 ccacgtcgac accgtgcgga aggcctggta agcctctcca gccttaccgg acagcacggc
    16381 gtgatgcagg tgcgcatgga tttgcgcgat gatgaggggt tgttaagcaa tggtcgcgtc
    16441 tggctacagg cggatgacat cgacctgaag ccgtggctcg gtaaatggat gcaggacaat
    16501 attgctctgg aaacggcaca gttctccctt gaaggctgga tgacgatcga caaaggcgat
    16561 gtaaccggcg gtgacgtctg gctgaaacag ggcggtgcca gctggttggg cgagaagcaa
    16621 acgcatacgc tgtcggtgga taatctgacc gcgcatatta cgcgtgaaaa tccgggctgg
    16681 cagttctcta ttcccgatac acggatcacg atggacggca aaccctggcc gagcggagca
    16741 ttgacgctgg cctggatacc ggaacaggac gttggcggca aagacaataa acgcagtgac
    16801 gaactccgga ttcgcgctag taatctggag ctggcaggcc tggagggcat acgcccgctg
    16861 gccgcgaaac tttcacctgc actgggtgat gtttggcgct ccacacaacc gagcggcaag
    16921 attaacactc tggcgctgga tatcccgctt caggcggcag acaagacccg ttttcaggca
    16981 tcgtggagcg atctggcctg gaagcaatgg aaattattac cgggtgcgga acacttctcc
    17041 gggacgcttt ccggcagcgt tgaaaatggt ttgcttaccg cgtcgatgaa gcaggcaaag
    17101 atgccttacg aaacggtatt ccgtgcgcca ctggaaatcg ccgacggcca ggcaactata
    17161 agctggctga acaatgacaa aggtttccag ctggatgggc gtaatattga cgttaaagcc
    17221 aaagccgtcc atgcgcgcgg cggttttcgt tacctgcaac ctgctaacga tgaaccctgg
    17281 ctgggtattc tggctggcat cagtaccgat gatggttcac aagcctggcg ctatttcccg
    17341 gaaaacttga tgggtaaaga cctggttgat tatttaagtg gtgcgattca gggcggtgaa
    17401 gcggataacg cgacgctggt ttatggtggc aatccgcaac tcttccccta taaacacaac
    17461 gaaggtcagt ttgaagtgct ggtgccgctg cgcaacgcga agtttgcctt ccagccggac
    17521 tggcctgcat taactaacct tgatattgaa ctggacttta ttaacgacgg tttatggatg
    17581 aaaaccgatg gcgttaatct gggcggcgtg cgcgcgagta atctcaccgc agtgatccct
    17641 gactactcaa aagaaaaact gctgattgac gctgacatta aaggtccggg taaagccgtt
    17701 ggcccttact ttgatgagac accgctgaaa gattctctgg gtgcgaccct gcaagaactc
    17761 cagctcgacg gcgatgtgaa tgctcgctta catcttgata tcccgctgaa cggcgaactg
    17821 gtaaccgcga aaggtgaagt gacgctgcgt aataacagtc tgtttatcaa accactcgac
    17881 agcatcctga aaaatttgag cggtaaattc agctttatca atggcgatct gcaaagtgaa
    17941 ccactgacag caagctggtt taatcagccg ttgaacgtgg atttttccac caaagaaggg
    18001 gcaaaagcct accaggttgc ggtgaatctc aacggtaact ggcaaccggc gaaaaccggc
    18061 gttctgcctg aagcggtgaa cgaagcattg agtggcagcg tggcgtggga tggtaaagtg
    18121 ggcattgatc tgccttatca tgctggcgcg acctataacg tagagctgaa cggcgatctg
    18181 aataatgtga gtagtcactt accttcaccg ttagccaaac ctgcgggtga accactagcg
    18241 gtaaacgtta aggttgatgg caatctcaac agctttgaat taaccggaca ggctggtgcg
    18301 gataaccatt tcaatagccg ctggttgctt ggtcaaaagc tgacgctcga tcgtgctatt
    18361 tgggcggcag acagtaaaac gctcccgccg ttgccggaac aaagtggcgt tgaacttaat
    18421 atgccgccga tgaatggtgc cgagtggctg gccctgttcc agaaaggcgc tgcggagagt
    18481 gtcggtggtg cagcgagttt cccacaacac ataacgttac gtacgcctat gttgtcactg
    18541 ggaaatcagc aatggaataa cctgagtatt gtttcgcaac cgacggcaaa tggcaccctg
    18601 gttgaggcgc aagggcgtga aatcaacgcc acgctagcga tgcgtaataa cgcgccgtgg
    18661 ctggcgaata tcaaatatct ttattacaac ccgagcgtgg cgaaaactcg tggtgattca
    18721 acgccgtcat cacctttccc gacaacggag cgcattaact tccgtggctg gccggacgca
    18781 caaatacgat gcacagagtg ctggttctgg gggcaaaaat tcggtcgcat tgacagtgat
    18841 atcaccattt ctggcgatac gttaacgctg accaatggac tgattgatac tggtttctcg
    18901 cggcttactg ccgatggtga atgggttaat aatccgggga atgaacgtac ctcgctgaaa
    18961 ggaaaactgc gtgggcagaa aattgatgcc gccgcagaat tttttggtgt cacgacgccc
    19021 atacgccagt cgtcatttaa tgtggattac gatttacact ggcgcaaagc accctggcag
    19081 ccagatgaag cgacgttgaa tggcatcatt catactcaac tgggtaaagg cgaaattacc
    19141 gaaatcaata ccggacatgc cgggcaattg ctgcgcttat tgagcgtaga tgccctgatg
    19201 cgtaagctgc gttttgattt cagagacact tttggcgaag ggttctattt tgactccatt
    19261 cgcagcaccg cgtggattaa agacggcgtt atgcacaccg acgacacgct ggtggatggc
    19321 ctggaggcgg atatcgccat gaaagggtcg gtaaatctgg tacgccgcga cctgaatatg
    19381 gaagcggttg tcgcaccaga gatttctgcg acggtgggcg tggctgcggc ttttgcggtt
    19441 aaccccattg ttggcgcggc agtgtttgcc gccagtaaag tgctggggcc gctgtggagc
    19501 aaagtctcca ttttgcgcta tcacatttcg ggtccgctgg acgatccgca aatcaacgaa
    19561 gtgttgcgcc aaccgcgtaa agaaaaagcg caatgatttg acgagggcgc gtaattgccc
    19621 caatctcata tgataatcgt tgccaaaggc caacgagccg gaacataact gtaggccgga
    19681 tagggcgttc acgccgcatc cggcagccgt aaaaaatcct ctactgcagt aactaacgag
    19741 tagcaaaaac gatgagtctg aacctggtaa gtgaacaatt gctagcggcg aacggcctga
    19801 aacatcagga cttgttcgcg atcctcggtc aactggccga acgtcgcctt gattatggcg
    19861 atctctattt tcagtcgagc tatcacgaat cctgggtttt agaagaccgc attattaaag
    19921 atggttctta caacatcgat cagggcgttg gcgtgcgtgc aatcagcggt gaaaaaaccg
    19981 gatttgctta cgctgaccaa atcagcctgc tggcgctgga acagagtgcg caagcggcgc
    20041 gcaccatcgt ccgtgatagt ggtgatggca aagtacagac gctgggcgcg gtagagcata
    20101 gcccgttgta tacctcggta gatccgctgc aaagcatgag ccgtgaagag aagctggata
    20161 tcctgcgtcg cgtcgataag gttgctcgcg aagcggacaa gcgcgtacag gaagtgactg
    20221 ccagcctcag tggtgtctat gaattaattc tggttgcggc caccgacggc acgctagcgg
    20281 cggatgtccg tccgctggtg cgtctttccg tgagcgttct ggtcgaagaa gatggcaaac
    20341 gcgaacgcgg tgccagtggc ggcggcggtc gttttggtta tgagttcttc cttgccgatc
    20401 ttgacggcga agtccgtgcg gatgcatggg caaaagaagc agtgcgtatg gcgctggtca
    20461 atctttctgc cgttgctgca ccagcgggca ccatgccggt agtacttggc gcaggttggc
    20521 cgggcgtgct gttgcatgaa gcggtaggtc acggtctgga aggcgacttc aaccgccgtg
    20581 gcacttcagt atttagtgga caggtcgggg agctggtggc ttcagaactg tgtaccgtgg
    20641 ttgatgacgg cacgatggtc gatcgccgtg gttcggtggc gattgatgat gaaggtacgc
    20701 caggccagta caacgtgctg attgagaacg gcattctgaa aggctacatg caggataaac
    20761 tcaacgcgcg tttgatgggg atgacgccga ctggcaacgg tcgccgtgaa tcctacgccc
    20821 atctgcccat gccgcgtatg accaacacct atatgctgcc gggtaaatcg accccgcaag
    20881 aaattattga atccgttgag tacggtatct atgcaccgaa ctttggtggc ggtcaggtgg
    20941 atatcacctc cggcaaattc gttttctcca cttcagaagc atatctgatt gaaaacggta
    21001 aagtaacgaa gccggtgaaa ggcgcaacgt tgattggttc cggtatcgaa accatgcagc
    21061 agatttcgat ggttggcaac gacctgaaac tggataacgg cgtgggtgtc tgcggtaaag
    21121 aagggcaaag tttgccggtt ggcgtgggcc agccaacgtt gaaagtcgat aacctgactg
    21181 ttggcggtac tgcgtaataa tttcattatt ttcagaagga taattaatct ttctacgtgc
    21241 acgaacggtc ccctcgctcc tctggggtta gggtgagggg aacccgttgg cacaggtttg
    21301 tagaacgtaa cagtacaata tgaattactt ctctttcccg cgcccgtgca tctcctgaaa
    21361 caatttaccg acctcaacaa aataatccgt cagcgagttg atcacgacct gtaccttcag
    21421 cggcagctta tctttttcgg tatataacgc ataaaccggg cgtggatctg actgataacg
    21481 cggcagcagg atctccagct ccccacgatt gatctcgttg atcacccaca tcagcggcac
    21541 gtaggcgatc ccggcacccg ccgttagcca gcgcaccagc gtcatcggat cattagtcac
    21601 aaatctccct tgcgggatca ggcgagtcga gatcccttcc ggtgcaatca gttcaaattc
    21661 attgtcgggc cgcacgctgt attcaagcca tgaatggcta cttaaatcgg cgggtttttc
    21721 cggtatgccg tattgtgtga gatagctttt cgcggcgcac accaccattg gcatcgcgcc
    21781 aagacggcgg gaaaacaggc tggaatcctg caacgcgccg acgcggatca ccacatccag
    21841 accgtcggca atcaggtcgg gggctggaat tccggtaacc agattggcgc tcaaacctgg
    21901 gtattctttc agcattttgg ctgtcagccc ggcgagaaca ttttgtgcca tagttgaaga
    21961 acagccaatg cgtagcgtcc cgatgggggt gttattgaag gcatacagtt gctcatgaac
    22021 atcctgcact tcatgaagca tacgacggca gccctggtag taaattctac cggcttcggt
    22081 caggccaatg ctgcgtgtgc tacggtttaa cagctttacc tgcaactcat cttccagttt
    22141 tgacaccgtc tgactgatgg acgaaacgct catctgtagc tgtctggcgg cggcggtaaa
    22201 agagccaaat tcaactactt tggcaaacac cgacatgcgt tttagtcgtt ccattattca
    22261 ctctgactta aaagtgattt agatcacata atatagataa cagcataaca gttacgctaa
    22321 tatattaaat atcaatctac agcaatgttg ctctcgcccg gctttccatg cccttctctg
    22381 cggcgacaga tgctgaaaat aataacgcct gctctctcta tacaaccaag gtcaacatga
    22441 gtctgtttcc cgttatcgtg gtgtttgggc tgtccttccc accgatattt tttgaattgc
    22501 ttttatcact ggcgattttc tggctggtgc gccgggtact tgtgccaaca ggtatctacg
    22561 actttgtctg gcatccggcg ttgttcaaca ccgcgctcta ttgctgcttg ttttatttga
    22621 tatcgcgact gttcgtttga ggttgaagtg aaaacactaa taagaaaatt ctcccgtacg
    22681 gccatcacgg tcgtattagt cattctggcc ttcatcgcaa tttttaatgc ctgggtctat
    22741 tacaccgaat ccccctggac gcgtgacgcg cgctttagcg ctgacgtcgt tgcgatcgcg
    22801 ccggacgttt ctggactcat tacccaggtg aatgttcatg ataaccagct ggtgaaaaaa
    22861 ggacagatac tgttcaccat cgaccagccg cgctatcaaa aggcgcttga ggaagcgcaa
    22921 gccgatgttg cttattatca ggtactggca caggagaaac gccaggaggc cggacgtcgt
    22981 aaccgtctcg gtgtgcaggc gatgtctcgc gaagagatcg accaggctaa caacgtacta
    23041 caaacggttc tgcatcagtt agcgaaagcg caggcgaccc gcgatctggc aaaactggat
    23101 cttgaacgca cggtgatccg agcgccagca gatggctggg tgaccaacct caacgtctat
    23161 accggtgagt ttattactcg aggatcaacg gcggttgcgc tggtgaaaca gaactccttc
    23221 tatgtactgg cctatatgga agaaactaag ctggaagggg tgcgtccggg gtatcgtgca
    23281 gagatcacgc cgcttggcag taacaaagtg ctgaaaggga ctgttgatag tgttgccgct
    23341 ggggtcacca acgccagcag cacgcgtgac gacaaaggga tggcgaccat cgactctgac
    23401 cttgaatggg tgcgtttggc gcaacgtgtt ccggttcgta ttcgtctcga caaccagcaa
    23461 gagaacatct ggcctgcggg caccactgct acagtggtgg tcactggcaa acaagatcgc
    23521 gacgaaagcc aggattcgtt cttccgtaaa atggcccatc gcctgcgtga gtttggttaa
    23581 tcacgatggg tattttctcc attgctaacc aacatattcg ctttgcggta aaactggcga
    23641 ccgccattgt actggcgctg tttgttggct ttcacttcca gctggaaacg ccacgctggg
    23701 cggtactgac agcggcgatt gttgccgccg gtccggcctt tgctgcggga ggtgaaccgt
    23761 attctggcgc tattcgctat cgtggctttt tgcgcatcat cggcacattt attggctgta
    23821 ttgccggact ggtgatcatc attgcgatga tccgcgcacc attattgatg attctggtgt
    23881 gctgtatctg ggccggtttt tgtacctgga tatcctcgct ggtacgaata gaaaactcgt
    23941 acgcgtgggg gctggccggt tataccgcgc tgatcattgt gatcaccatt cagccggaac
    24001 cattgcttac gccgcagttt gccgtcgaac gttgtagcga gatcgttatc ggtattgtgt
    24061 gtgcgattat ggcggatttg ctcttttctc cgcgatcgat caaacaagaa gtagatcgag
    24121 agctggaaag tttgctggtc gcgcaatatc aattaatgca actctgtatc aagcatggcg
    24181 atggtgaagt tgtcgataaa gcctggggcg acctggtgcg acgcaccacg gcactacaag
    24241 gcatgcgcag caacctgaat atggaatctt cccgctgggc gcgggccaat cgacgtttaa
    24301 aagcgatcaa tacgctatcg ctgacgctga ttacccaatc ctgcgaaact tatcttattc
    24361 agaatacgcg cccggaattg atcactgata ctttccgcga attttttgac acgccggtag
    24421 aaaccgcgca ggacgtccac aagcagctca aacgcctgcg gagagttatc gcctggactg
    24481 gggaacggga aacgcctgtc accatttata gctgggtcgc ggcggcaacg cgttatcagc
    24541 ttctcaagcg cggcgttatc agtaacacaa aaatcaacgc caccgaagaa gagatcctgc
    24601 aaggcgaacc ggaagtaaaa gtagagtcag ccgaacgtca tcatgcaatg gttaacttct
    24661 ggcgaaccac actttcctgc attctgggca cgcttttctg gctgtggacg ggctggactt
    24721 ccggcagtgg tgcaatggtg atgattgcgg tcgtgacgtc actggcaatg cgtttgccga
    24781 atccacgcat ggtggcgatc gactttatct atgggacgct ggccgcgctg ccgttagggc
    24841 tgctctactt tttggtgatt atccctaata cccaacagag catgttgctg ctgtgtatta
    24901 gcctggcagt gctgggattc ttcctcggta tagaagtaca gaaacggcga ctgggctcga
    24961 tgggggcact ggccagcacc ataaatatta tcgtgctgga taacccgatg actttccatt
    25021 tcagtcagtt tctcgacagc gcattaggac aaatcgtcgg ctgtgtgctc gcgttcaccg
    25081 ttattttgct ggtgcgggat aaatcgcgcg acaggactgg acgtgtactg cttaatcagt
    25141 ttgtttctgc cgctgtttcc gcgatgacta ccaatgtggc acgtcgtaaa gagaaccacc
    25201 tcccggcact ttatcagcag ctgtttttgc tgatgaataa gttcccaggg gatttgccga
    25261 aatttcgcct ggcgctgacg atgattatcg cgcaccagcg cctgcgtgat gcgccgatcc
    25321 cggttaacga ggatttatcg gcgtttcacc gacaaatgcg ccgcacagca gaccatgtga
    25381 tatctgcccg tagcgatgat aaacgtcgtc ggtactttgg ccagttgctg gaagaactgg
    25441 aaatctacca ggaaaagcta cgcatctggc aagcgccacc gcaggtgacg gaaccggttc
    25501 atcggctggc ggggatgctc cataagtatc aacatgcgtt gaccgatagt taagtcaaaa
    25561 ccgacgccaa aagcgtcggt tttttcatgg ctatacttag cgatgcacgg cagaactcgc
    25621 cgcgaaacgt gacggtggca acagatgaat atttatgcct ttgattttga tgagattgag
    25681 agtcaggagg atttttatcg tgactttagc caaacctttg gtctggctaa agataaggta
    25741 cgcgatctcg actcactatg ggatgtgtta atgaacgatg tcctgccgct accacttgag
    25801 attgaatttg ttcatctggg agagaaaacg cgtcgccgtt ttggcgcgtt aatattgctg
    25861 tttgatgagg cagaggaaga gctggaaggg catttgcgtt ttaatgttcg tcattagcgc
    25921 aaaaaaaagc ccacgaaccg ggggcaatat cgtcggacaa aacgatgagg gtttatttgt
    25981 acagttcagc cgtagcgtgc caggtgtcac cgctacgagc ttcagtaatc tggtaggccg
    26041 ttgcgccttt ctcttccgct tttttgttca gcatttcacg tatatccatt ggcgaagacg
    26101 ccacaccact tacggatacg gtcccgattg cttcacgatt ttgtgcttgt gcagcatcaa
    26161 tggagtcggc agcgaatgca ccgaaagaga gaacagaaag tacgcttaat gcagctacag
    26221 tggttttgat tttcatgatt tttacctcga cataatcttt tagctgggcc tttgtttcgt
    26281 gacccatatc acaaaatcaa gtatacacta attattgaac taattaatac cacactaact
    26341 gtttcagttg atactaagtg ttaaaaatgt gaattttaat aacaaaaaag tataaaaaac
    26401 acggttattt attattaatt atagtatttt caattagata atgaattttg cataatgtgc
    26461 tttttttacc gtatgtattc attttgtgaa tgatgtgtcg cggtaaaacg cactattcgt
    26521 taaatgtatc gcaggagaaa gaaggggaat gagagggata agcaacattt tccccgccgc
    26581 cagaagcgac ggggcagaga ttaaagctcc tggtcgaaca gctctaaaat cgcttcgtac
    26641 aggtctttga cggtgaaacc gttagcaggg gtggtaaaga tggtgtcatc gccagcgatg
    26701 gtgcccagaa taccttctgc tttgcccagt gagtccagca ggcgagcaat taactgcgcc
    26761 gcgccagggc tggtatgaat cacgacaact gcatcgttgt agtcgatatc cagcaccaga
    26821 ttcttcaatg gactggaggt ggttggtaca cccagttcag ctggcaggca gtaaaccatt
    26881 tccattttgg cattgcgtgt acgtacagca ccaaacttgg tcaacatccg cgagacttta
    26941 gactgattaa tattgtcaaa gccttgctcc tgcaacgcgg cgacgatttc gccctgggag
    27001 ctaaatttct cttctttaag taatgcttta aatgctttaa ctagttcttc ttgcttagcc
    27061 gagcttcgca taagtcaccc gatatggtgg ttgatacaac attattgtgc atacagatga
    27121 atttttatgc aaacagtcag ccctgaagaa ggctgaaata atgttatgaa agagcgggat
    27181 tttatcaaat ttcgttattg agaaacatgc ctgcgtcacg gcatgcaaat tctgtttaaa
    27241 agtaaattaa ttgttatcaa attgatgttg ttttggctga acggtagggt atattgtcac
    27301 cacctgttgg aatgttgcgc taatgcataa gcgactgtta attacgtaag ttaggttcct
    27361 gattacggca attaaatgca taaacgctaa acttgcgtga ctacacattc ttgagatgtg
    27421 gtcattgtaa acggcaattt tgtggattaa ggtcgcggca gcggagcaac atatcttagt
    27481 ttatcaatat aataaggagt ttaggatgaa agtcgcagtc ctcggcgctg ctggcggtat
    27541 tggccaggcg cttgcactac tgttaaaaac ccaactgcct tcaggttcag aactctctct
    27601 gtatgatatc gctccagtga ctcccggtgt ggccgtcgat ctgagccata tccctactgc
    27661 tgtgaaaatc aaaggttttt ctggtgaaga tgcgactccg gcgctggaag gcgcagatgt
    27721 cgttcttatc tctgcaggcg tagcgcgtaa accgggtatg gatcgttccg acctgtttaa
    27781 cgttaacgcc ggcatcgtga aaaacctggt acagcaagtt gcgaaaaact gcccgaaagc
    27841 gtgcattggt attatcacta acccggttaa caccacagtt gcaattgctg ctgaagtgct
    27901 gaaaaaagcc ggtgtttatg acaaaaacaa actgttcggc gttaccacgc tggatatcat
    27961 tcgttccaac acctttgttg cggaactgaa aggcaaacag ccaggcgaag ttgaagtgcc
    28021 ggttattggc ggtcactctg gtgttaccat tctgccgctg ctgtcacagg ttcctggcgt
    28081 tagttttacc gagcaggaag tggctgatct gaccaaacgt atccagaacg cgggtactga
    28141 ggtggttgaa gcgaaagccg gtggcgggtc tgcaaccctg tctatgggcc aggcagctgc
    28201 acgttttggt ctgtctctgg ttcgtgcact gcagggcgaa caaggcgttg tcgaatgtgc
    28261 ctacgttgaa ggcgacggtc agtacgcccg tttcttctct caaccgctgc tgctgggtaa
    28321 aaacggcgtg gaagagcgta aatctatcgg taccctgagc gcatttgaac agaacgcgct
    28381 ggaaggtatg ctggatacgc tcaagaaaga tatcgccctg ggcaaagagt tcgttaataa
    28441 gtaattgatt agcgaataat aaaaaaccgg agcacagact ccggtttttg ttttgagcac
    28501 tcgacttaat tggttgccgg atattcctga atggtgacct gcagcgttaa ctgcttatca
    28561 tcacgcatca ctacaaccgg gatcaccgaa ccagggcgaa tttccgccac ctgatccatc
    28621 gtctccagag cagagatggc cggtttgtta tccaccgaaa taatcagatc gttgacctga
    28681 atacccgcat tcgccgctgg gccgtcaggt gacacttcat taaccacgat cccttgcagt
    28741 tgatctatac caccgctctg cgcgtgcagt ggtgcgatct cgcgtccgcc gataccaatg
    28801 tagccgcgga tcacgcgacc atcgcggatc agcttatcca taattttggt tgctaactgg
    28861 aaaggaatcg caaagccgat accttccggc gtttcgccat cgttactctt atcaaacgac
    28921 agcgtgttaa tgcccatcag ttctcccagc gagttcacca gcgcgccgcc agagttaccg
    28981 tggttaatgg aagcatcggt ttgtaggaag ttttgccgcc cggtcgggtt cagaccgatt
    29041 cgacccgtgg cactaataat cccttgggta atggtctgcc cgaggttgta cgggttaccg
    29101 atcgccagta ctacgtcgcc aatgtgcggt acgcgacgtg cattaattgg aatggtaggt
    29161 aaaccgccag tggcattaat tttaagtacc gccagatcgg ttagagagtc agatcccacc
    29221 agcaatgctt caaatacacg tccatcctgt aaggcgacga tgatctgatc ggcgtcgttg
    29281 atgacgtgtt tattggtgat gatataaccg cgttgatcca tgattacacc ggatcccagg
    29341 gtgcggatct caagctggtt gtgagagttg gtgttcaaac cacggttgta aacgttaacc
    29401 accgctggcg cggcgcggcg aaccgccaga ttatagctgg caggcgtctc atcggtactg
    29461 tcaaattgcg gagtggaaag cgggttaagg ctgcgcagcg aaggcatggc aaccagcaga
    29521 atagcgccga caattaatcc aatcgcaacg gaacgtaaga gcttcacaaa catgatggag
    29581 gcgtcattaa aaaagggaac ggcagcagca taccacgagt taaccggaca tcacacgtca
    29641 gcctgatgcc cggtttacga cattaacgca tcagcagata gatgctttca ttgccgcgta
    29701 caatttgcag ggcgatgatg gccggttttg ccgccagcac tttacgcatt tcagcaatcg
    29761 agttcacccg atcgcggttg acgccaatga tcacatcgtc tttttgcaag ccagcctgag
    29821 cagctgggct tcctttgaca acttcatcta tcttaatacc tttgccgcca tcttttagct
    29881 gaccatcgct caacgttgca ccttccagcg ctggccgacg aagaggtgct ggtatcaagc
    29941 gtcacttcta cttccagtgg tttgccgtta cgcagcaggc caagcttcac tttcgtgccc
    30001 ggctctgtag tcgcgatacg agagcgcaac tcagcaaagc tattcagcgg tttgccgttg
    30061 aggctggtaa taatatcgcc cgctttgacg cccgctttcg ctgagccaga acttggcaac
    30121 acttcgctga caaacgcgcc acgctgcacg tcaaggttga aggctttggc gatatcggca
    30181 ctcatctcgg tgcctttgat gcctaacaaa ccgcgtttga tttcaccaaa gtcgataagc
    30241 tgctgcgcca gtgttcgcgc catattactg gggatggcaa atccaatccc gacgctcccg
    30301 ccgccaggcg caaggattgc agtgttgatg ccaattaact caccgttaag gtttaatagt
    30361 gcaccgccgg agttaccgcg gttaatggaa gcatctgtct ggataaagtt ttccagacct
    30421 tcaagattca acccgctgcg gcctaatgcg gaaacaatgc cagaggtggc ggtttgccca
    30481 aggccaaatg ggttaccgac cgctacggca aaatcaccga cgcgcaattt atcggagtcg
    30541 gcaatagcga tttgcgttaa tttgctcggg ttttgaattt gtaacagggc gatatcgctc
    30601 tggtcatcgc taccaatcag ttttgcatca aactcgcgcc catcattgag ctgaatactg
    30661 attttctgtg cctgattaat cacatggttg ttggtcagca catagccttt actggcgttg
    30721 atgatgacac cggaacctaa accttcgaag ggttgtgcag gttgatccgg taaatcatca
    30781 ccaaaaaact ttttgaattc ttccgggatt ttctgtccct gactggccgt tccttccacc
    30841 cgtacgctca ccactgccgg aagcactttt tccagcattg gagccagact ggggagaggg
    30901 gcctgatcgg caacctggcc tggaatcgac gcgacggcct gaaatgacgc cgagagagtt
    30961 aacccggcac ttaacgctaa tgcactcaac agctgggttt gttttttcat tattcctgct
    31021 ctcgtacctg aatgataaga aaagagattc aaaacagttt gatgttattg aattttcaga
    31081 cggctaacaa tgagacacca gattaaatga tagtcgggga gggagagaag aggagggcgc
    31141 aatggctgcg cccgaaaaat aaattagtcg cgcttcgcgc cagtacgcag caggccggat
    31201 gcgccttcag aatagtcgcg aggcacctgc accggtgcct gatcgttgct ggcttcagac
    31261 tctgccagac gattacggaa cgggtttgct tcagcagaca gttccggcag caggctgctg
    31321 gagctttttg ccatgtgctg atacagctgg cgatagtcgt gcgccatggt atccagtaat
    31381 tccgcgctgc gggcaaagtg gctaaccagc tcttcgcgat actcgtccag ttcagcttta
    31441 ttcttttcca gttcgtactg caacgcctgt tgctggcgta gtttacgatt accaaaacgc
    31501 atggccacag caccaataat gatgccgacg actaacccaa ttagcgcata ttcccaggtc
    31561 atgaacatct cccgttgtct tttgtttccg tagggtgttg gcttcaggct ccatgcctgc
    31621 ggctgattat gccactataa ccgttaattc cacagaagtg gaatcccgac tgcatatcgc
    31681 gtagtgtaga acggcctttt tttcgtcaac cgtgaacaac ggcgcaccga ttattcaagg
    31741 aataacaata agatcatgca aagcgttacc ccaacatcgc aatacctgaa ggcgctcaat
    31801 gaaggcagcc atcaacccga cgacgttcaa aaagaggccg tcagccgcct ggaaattatt
    31861 tatcaagaac tcatcaatag caggccacca gcccccagga cgagtgggct aatggcgcgg
    31921 gtcggtaagc tgtggggtaa acgcgaagac acaaagcata cgccagtgcg tggcttatat
    31981 atgtggggcg gtgtaggacg cgggaaaacc tggctgatgg accttttcta tcaaagcctg
    32041 ccgggagagc ggaaacagcg cctgcacttt caccgtttta tgctgcgggt gcacgaagag
    32101 ctaactgcct tacaggggca gaccgatccg ctggaaatta ttgccgatcg ctttaaagcc
    32161 gaaactgacg tgctctgttt tgacgaattt tttgtttctg atattaccga cgccatgcta
    32221 cttggcggtc tgatgaaagc cctgttcacc cgcggtatta ccctggtagc gacgtcaaat
    32281 attccgccgg acgaacttta tcgaaatggc ctgcaacgtg cgcgttttct gcctgcaatc
    32341 gatgccatta aacagcattg tgatgtaatg aacgtggacg ctggtgttga ttatcgactg
    32401 cgtacactca ctcaggcgca tctgtggctt tcgccactca acgatgaaac ccgggcgcag
    32461 atggataaac tatggttggc gctggcgggg gtgaaacgag aaaattcacc gacgttagaa
    32521 atcaaccatc ggccattggc gacaatgggc gtcgagaacc agacgctggc ggtttctttt
    32581 actacgctgt gcgtcgacgc ccgcagtcag catgactata ttgcgctctc acgcctgttt
    32641 cacacagtaa tgttgtttga tgtgccagtc atgacgcggt tgatggagag cgaagcgcgg
    32701 cgctttattg cgctggtgga tgagttttac gagcgccatg tcaaattagt ggtgagtgca
    32761 gaagtgccgc tgtatgaaat ttatcagggt gagcggctga agtttgagtt ccagcgttgc
    32821 ctgtcacgtc tgcaagagat gcaaagcgaa gagtatctga agcgcgagca tttggcgggt
    32881 taaaacctgc cacaaatcac aaaaaggggt cgatctttga ccccgacttc tctataatcc
    32941 tgcgacccca cgttacaaga aagttttttt cccaaaactt tttgtgtgct ggcataggct
    33001 attcgaaggg gtaggtttgc cggactttgt cgtgtgaacc tcaacaattg aagacgtttg
    33061 ggtgttcacc aacgtgtaac tatttattgg gtaagctttt aatgaaaact tttacagcta
    33121 aaccagaaac cgtaaaacgc gactggtatg ttgttgacgc gaccggtaaa actctgggcc
    33181 gtctggctac tgaactggct cgtcgcctgc gcggtaagca caaagcggaa tatactccgc
    33241 acgtagatac cggtgattac atcatcgttc tgaacgctga caaagttgct gtaaccggca
    33301 acaagcgtac tgacaaagtg tactatcacc acaccggcca catcggtggt atcaaacaag
    33361 cgacctttga agagatgatt gctcgccgtc ctgagcgtgt gattgaaatc gcggttaaag
    33421 gcatgttgcc aaaaggcccg ctgggtcgtg ctatgttccg taaactgaaa gtttacgcgg
    33481 gtaacgagca caaccacgcg gcacagcaac cgcaagttct tgacatctaa tcgggattat
    33541 aggcaatggc tgaaaatcaa tactacggca ctggtcgccg caaaagttcc gcagctcgcg
    33601 ttttcatcaa accgggcaac ggtaaaatcg taatcaacca acgttctctg gaacagtact
    33661 tcggtcgtga aactgcccgc atggtagttc gtcagccgct ggaactggtc gacatggttg
    33721 agaaactgga cctgtacatc accgttaaag gtggtggtat ctctggtcag gctggtgcga
    33781 tccgtcacgg tatcacccgc gctctgatgg aatacgacga gtccctgcgt tctgaactgc
    33841 gtaaagctgg cttcgttact cgtgacgctc gtcaggttga acgtaagaaa gtcggtctgc
    33901 gtaaagcacg tcgtcgtccg cagttctcca aacgttaatt ggcttctgct acggcagaaa
    33961 acaatttgcg aaaaaacccg cttcggcggg tttttttatg gctaaattct gaatcagcgt
    34021 aaaaaatggg aagttgcttt ttgctgccgt ctgaaagaca ggtaaaacaa aaaccacatc
    34081 gccaataagg gtctaagtca actatttcag actaaagcgc atctcttttt ccccatttcc
    34141 ggcatccact caccacaaag gtcgcaaaat ctggtaaact atcatccaat tttctgccca
    34201 aatgtcgggt attgctcatt ttttgtttga ttttcgaaca aagagagtcg ttccttattg
    34261 ggtaacacaa cttctgactg gccacctggt ggctggtagc agtaaaaatt ctgactatac
    34321 ctggaggttt tcatggctgt cgctgccaac aaacgttcgg taatgacgct gttttccggt
    34381 cctactgaca tctatagcca tcaggtccgc attgtgctgg ctgagaaagg tgtaagtttc
    34441 gagatcgaac acgtggaaaa ggacaatccg cctcaggatc tgattgacct caacccgaat
    34501 cagagcgttc cgaccctggt ggatcgtgag ctgaccctgt gggaatctcg catcattatg
    34561 gaatatctgg atgagcgttt cccgcatccg ccactgatgc ctgtttaccc ggtagctcgc
    34621 ggtgaaagcc gtctgtacat gcatcgcatc gaaaaagact ggtacacgct gatgaacact
    34681 atcatcaacg gttcagcttc tgaagcagat gccgcacgta agcaactgcg cgaagaactg
    34741 ctggcgattg cgccggtctt cggtcagaag ccgtacttcc tgagcgatga gttcagcctg
    34801 gtcgattgct accttgctcc gctgctgtgg cgtctgccgc aactgggcat cgagttcagc
    34861 ggcccgggtg cgaaagagct gaaaggctat atgacccgcg tctttgagcg tgactctttc
    34921 cttgcttctt taactgaagc agaacgtgaa atgcgtctgg gccggagtta atctgtatgg
    34981 atttgtcaca gctaacacca cgtcgtccct atctgctgcg tgcattctat gagtggttgc
    35041 tggataacca gctcacgccg cacctggtgg tggatgtgac gctccctggc gtgcaggttc
    35101 ctatggaata tgcgcgtgac gggcaaatcg tactcaacat tgcgccgcgt gctgtcggca
    35161 atctggaact ggcgaatgat gaggtgcgct ttaacgcgcg ctttggtggc attccgcgtc
    35221 aggtttctgt gccgctggct gccgtgctgg ctatctacgc ccgtgaaaat ggcgcaggca
    35281 cgatgtttga gcctgaagct gcctacgatg aagataccag catcatgaat gatgaagagg
    35341 catcggcaga caacgaaacc gttatgtcgg ttattgatgg cgacaagcca gatcacgatg
    35401 atgacactca tcctgacgat gaacctccgc agccaccacg cggtggtcga ccggcattac
    35461 gcgttgtgaa gtaatacaaa acaggcccag gcggcctgtt ttgtcttttt aatgacttaa
    35521 taaaatagcg tgattgtggc aatcatattc accacgaaac cgactgccat ggggatcgct
    35581 gtccgcttca ccaccgcaaa aggcgaaacg cctgcaattc ctgacgtaac gaccaccacc
    35641 gcagtaatcg gcgaaaccgc gcgcgctagc gtcgtggcaa aatgcatcgg cataatcatt
    35701 acaaccgctg gtacatgtag tccggctgcg atattcggaa taagactggc aaatgacata
    35761 aacggcgcat tgccagagcc catcacaatg gcacaaatgg caatgactag cgccataata
    35821 atcatcacgc caataccgcc cagaccagaa tgctccgcac ccctgataac cgcatcaaca
    35881 gtgccaatcg tcgttaagcc tttcgcaaat atttcacccg cgaccaccag cgttaccacg
    35941 ttgacaaact gcgtacccat gccgtcaaaa aacgcctgca catcgtccat tgtttcgcgc
    36001 aagttatgct tgcggaagaa ctcaacaaac atcgtcaccg tcaaacttag taacatcacc
    36061 accaccagat gcagttctga ctgcatcagc ccgacgtggg cgaggaacag cgagccgagc
    36121 atcaggatta acggcatcac aggtaaaatg gcgtaataga gcggcgggac attatcgaga
    36181 gttttttgct ctgcctgttc gtgattgata tttttatctt ttttgtcaaa agcgcgttgc
    36241 acgaaaaagt gggagatcgc caccgagata atgacgcaag aggcgaccgg aagctggtag
    36301 tggaagaagt atgtggcaat tttcattccc gcgacctggg ctgcaaaaat ggagttcgtt
    36361 tccagaatcc cccactcaat ggacatcgtg gttgcgataa ccgccaccgc agagaggcga
    36421 cttactccca gactcaccag cgtcggaaat aaggtgacca tcagcaacat acccagaccg
    36481 gaggcgctgg taataaactg cgccatgatt tggccgatga cgtaagttgc cgacagaata
    36541 atatacggcg agcgaatgag ttttaacggg cggcttagca agctcaccat cgcgcaactg
    36601 gctcctatgc gctccatgta gcgggcataa ccgcccaccg ccataatcga cagccccaat
    36661 ccggcaatgc ggttggacag catgcgcagg atttcattat aaatgtcgac gatcaagtag
    36721 ccgctgcttt tggcgggatc gagtacacca ccgaacccta accagactcc gcacatcatc
    36781 aggaagacac ctgcggcagc cagcaccacc tgaggtttgt agtttttcag gatcaaatag
    36841 cccatcgtaa ttaatacgat gacagatata attatgccga aca
//
LOCUS       DAJTQQ010000032        36246 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000032 DAJTQQ010000000
VERSION     DAJTQQ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36246
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.032"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..1040)
                     /locus_tag="OO309_001769"
     CDS             complement(<1..1040)
                     /locus_tag="OO309_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5846936.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKA"
     gene            complement(1187..1263)
                     /locus_tag="OO309_001770"
     tRNA            complement(1187..1263)
                     /locus_tag="OO309_001770"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1227..1229),aa:Pro,seq:tgg)
     gene            complement(1306..1392)
                     /locus_tag="OO309_001771"
     tRNA            complement(1306..1392)
                     /locus_tag="OO309_001771"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1356..1358),aa:Leu,seq:cag)
     gene            complement(1413..1488)
                     /locus_tag="OO309_001772"
     tRNA            complement(1413..1488)
                     /locus_tag="OO309_001772"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1453..1455),aa:His,seq:gtg)
     gene            complement(1547..1623)
                     /locus_tag="OO309_001773"
     tRNA            complement(1547..1623)
                     /locus_tag="OO309_001773"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1587..1589),aa:Arg,seq:ccg)
     gene            complement(1726..3111)
                     /locus_tag="OO309_001774"
     CDS             complement(1726..3111)
                     /locus_tag="OO309_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5846937.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3302..4042)
                     /gene="rffM"
                     /locus_tag="OO309_001775"
     CDS             complement(3302..4042)
                     /gene="rffM"
                     /locus_tag="OO309_001775"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR5846938.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4045..5397)
                     /gene="wzyE"
                     /locus_tag="OO309_001776"
     CDS             complement(4045..5397)
                     /gene="wzyE"
                     /locus_tag="OO309_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR5846939.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5394..6473)
                     /gene="rffT"
                     /locus_tag="OO309_001777"
                     /gene_synonym="wecF"
     CDS             complement(5394..6473)
                     /gene="rffT"
                     /locus_tag="OO309_001777"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR5846940.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6470..7720)
                     /gene="wzxE"
                     /locus_tag="OO309_001778"
     CDS             complement(6470..7720)
                     /gene="wzxE"
                     /locus_tag="OO309_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR5846941.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(7722..8852)
                     /gene="rffA"
                     /locus_tag="OO309_001779"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(7722..8852)
                     /gene="rffA"
                     /locus_tag="OO309_001779"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR5846942.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(8857..9531)
                     /gene="rffC"
                     /locus_tag="OO309_001780"
                     /gene_synonym="wecD"
     CDS             complement(8857..9531)
                     /gene="rffC"
                     /locus_tag="OO309_001780"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR5846943.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9509..10390)
                     /gene="rfbA"
                     /locus_tag="OO309_001781"
     CDS             complement(9509..10390)
                     /gene="rfbA"
                     /locus_tag="OO309_001781"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5846944.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10409..11476)
                     /gene="rffG"
                     /locus_tag="OO309_001782"
     CDS             complement(10409..11476)
                     /gene="rffG"
                     /locus_tag="OO309_001782"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5846945.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11476..12738)
                     /gene="wecC"
                     /locus_tag="OO309_001783"
     CDS             complement(11476..12738)
                     /gene="wecC"
                     /locus_tag="OO309_001783"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR5846946.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(12735..13865)
                     /gene="wecB"
                     /locus_tag="OO309_001784"
     CDS             complement(12735..13865)
                     /gene="wecB"
                     /locus_tag="OO309_001784"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR5846947.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(13921..14967)
                     /gene="wzzE"
                     /locus_tag="OO309_001785"
     CDS             complement(13921..14967)
                     /gene="wzzE"
                     /locus_tag="OO309_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR5846948.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(14979..16082)
                     /gene="wecA"
                     /locus_tag="OO309_001786"
     CDS             complement(14979..16082)
                     /gene="wecA"
                     /locus_tag="OO309_001786"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR5846949.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16322..17581)
                     /gene="rho"
                     /locus_tag="OO309_001787"
     CDS             complement(16322..17581)
                     /gene="rho"
                     /locus_tag="OO309_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR5846950.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(17666..17767)
                     /gene="rhoL"
                     /locus_tag="OO309_001788"
     CDS             complement(17666..17767)
                     /gene="rhoL"
                     /locus_tag="OO309_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR5846951.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(17908..18237)
                     /gene="trxA"
                     /locus_tag="OO309_001789"
     CDS             complement(17908..18237)
                     /gene="trxA"
                     /locus_tag="OO309_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR5846952.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18368..19633
                     /gene="rhlB"
                     /locus_tag="OO309_001790"
     CDS             18368..19633
                     /gene="rhlB"
                     /locus_tag="OO309_001790"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR5846953.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            19767..21251
                     /gene="gppA"
                     /locus_tag="OO309_001791"
     CDS             19767..21251
                     /gene="gppA"
                     /locus_tag="OO309_001791"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR5846954.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21298..23319)
                     /gene="rep"
                     /locus_tag="OO309_001792"
     CDS             complement(21298..23319)
                     /gene="rep"
                     /locus_tag="OO309_001792"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR5846955.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23406..23687
                     /gene="ppiC"
                     /locus_tag="OO309_001793"
     CDS             23406..23687
                     /gene="ppiC"
                     /locus_tag="OO309_001793"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR5846956.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(23733..25208)
                     /gene="ilvC"
                     /locus_tag="OO309_001794"
     CDS             complement(23733..25208)
                     /gene="ilvC"
                     /locus_tag="OO309_001794"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR5846957.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25358..26248
                     /gene="ilvY"
                     /locus_tag="OO309_001795"
     CDS             25358..26248
                     /gene="ilvY"
                     /locus_tag="OO309_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR5846958.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26245..27789)
                     /gene="ilvA"
                     /locus_tag="OO309_001796"
     CDS             complement(26245..27789)
                     /gene="ilvA"
                     /locus_tag="OO309_001796"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR5846959.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(27792..29642)
                     /gene="ilvD"
                     /locus_tag="OO309_001797"
     CDS             complement(27792..29642)
                     /gene="ilvD"
                     /locus_tag="OO309_001797"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR5846960.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(29707..30636)
                     /gene="ilvE"
                     /locus_tag="OO309_001798"
     CDS             complement(29707..30636)
                     /gene="ilvE"
                     /locus_tag="OO309_001798"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR5846961.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(30656..30919)
                     /gene="ilvM"
                     /locus_tag="OO309_001799"
     CDS             complement(30656..30919)
                     /gene="ilvM"
                     /locus_tag="OO309_001799"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR5846962.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(30916..32562)
                     /locus_tag="OO309_001800"
     CDS             complement(30916..32562)
                     /locus_tag="OO309_001800"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR5846963.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32565..32615)
                     /locus_tag="OO309_001801"
     CDS             complement(32565..32615)
                     /locus_tag="OO309_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846964.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(32701..32799)
                     /gene="ilvL"
                     /locus_tag="OO309_001802"
     CDS             complement(32701..32799)
                     /gene="ilvL"
                     /locus_tag="OO309_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR5846965.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33151..34630
                     /locus_tag="OO309_001803"
                     /pseudo
     CDS             33151..34630
                     /locus_tag="OO309_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(34655..34993)
                     /gene="maoP"
                     /locus_tag="OO309_001804"
     CDS             complement(34655..34993)
                     /gene="maoP"
                     /locus_tag="OO309_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR5846966.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35112..35951
                     /gene="hdfR"
                     /locus_tag="OO309_001805"
     CDS             35112..35951
                     /gene="hdfR"
                     /locus_tag="OO309_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR5846967.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     DVY"
     gene            complement(36047..36122)
                     /locus_tag="OO309_001806"
     tRNA            complement(36047..36122)
                     /locus_tag="OO309_001806"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36087..36089),aa:Trp,seq:cca)
     gene            complement(36131..36207)
                     /locus_tag="OO309_001807"
     tRNA            complement(36131..36207)
                     /locus_tag="OO309_001807"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36171..36173),aa:Asp,seq:gtc)
BASE COUNT         8851 a         9914 c         9136 g         8345 t
ORIGIN      
        1 gcttttaaca cgttacaact gcgacactgc gccggtaatt gcttaaattt atcttcacca
       61 aacacctgct gttgcgggga atcgatcatt tctgcaattg tttgctgatg catattcccc
      121 agccgatatt gcggataaac atagtgatcg caggcgtaaa cgtcgccgtt gtgctcaaca
      181 atcaccgagc gcccacaggt tggctgatga tggcaaaccg cacccggcgc accgacaaaa
      241 ttggcaaacg cccattcgat attcatcacg aaaatcttgc cgacgtcgcg tttgatccag
      301 tggtcaaaaa tcgccaccag aaattcacca aattcatcgg ggcgcacaga ccattccgtc
      361 agttccccct gaatatcacc aggcgcatgt agtttcagtc catcgcggac agctgtttca
      421 tcagccaggc gctcgaccac cggaataaac tggatgaatt cgactcccgc atcgcacaaa
      481 aaatcataaa cctgtaacgg ttgctgcgcg ctggtgcggt tgacgcagac cagcacgtta
      541 tagtcgacat gatgtttttg caggagcgtc agggcacgca tcaccagttt atgcgtgggt
      601 ctgccgcctt tggtcacgcg atattgattg tggatctcag ccggaccatc cagcgataac
      661 ccaacaagaa aattattttc tgccagaaat gcacaccatt cgtcatcgag cagcacgccg
      721 ttagtctgga aactgttact tatcttcctg ccagcaccat atttcgcctg tagcgccacg
      781 gcacggcggt aaaactccag cccgagtagc gttggttcgc cgccctgcca ggtaaaagcc
      841 acttcgtttt gcagttcgct ggcggcgata tagtgacgga tatacgcttc cagcgtgtca
      901 tcgtccatat gcgtgactgg cttttcgcgg taaagggatt gtttttcgag ataaaaacag
      961 tagtcacagt tcagattaca atcggaaccg ctcggtttcg ctatcacatg aaaagcacgc
     1021 gttggaacct gttgcagcat ggtcgctcct tgcctgaagt catccttcaa tacgttaagg
     1081 gcgaaatcat accgtggggc aatgagttat tacgggcgtt tattgcgaag ggagggtgac
     1141 gaaatgcaca gaaaacaaaa aaccccgccg aagcggggtt caaaattggt cggcgagaga
     1201 ggattcgaac ctccgaccca ctggtcccaa accagttgcg ctaccaagct gcgctactcg
     1261 ccgaaatact gctttttgaa tttttagttc aattctttaa agtcgtggtg cgaggggggg
     1321 gacttgaacc cccacgtccg taaggacact aacacctgaa gctagcgcgt ctaccaattc
     1381 cgccaccttc gcattgtcac aacttctaat aatggggtgg ctaatgggat tcgaacccac
     1441 gacaactgga atcacaatcc agggctctac caactgagct atagccacca ctacaaatct
     1501 tgttacgcgg tattactacc accgcagctc aagcgctggg actaaatggc gcgcccgaca
     1561 ggattcgaac ctgagacctc tgcctccgga gggcagcgct ctatccagct gagctacggg
     1621 cgcttagcgc cgttgcgggg gtggataata cggacttccc acccctctgt ctagtccctt
     1681 tttaaataaa ttgctcgttt ggttacggtt tgtgcgtttt gctgcttatt cctccagttt
     1741 atgcgcttta ccgtggcgat taagcccaaa aactttataa atcgccgtca ccgccagcat
     1801 aaagatgatg ccgacaaaca gcgacatacg cgtatcttca ttaaagtaca tgccgatcaa
     1861 aacgcaaatc aggaatgcca ttgttacgta gttggcccac gggaacagga ttgagcggaa
     1921 cggatggctg gcaatcgccg ctttatgtgc acgccgaaaa cgcagctggc ttatcaatat
     1981 cacaaaccat ggcaccatcc ccggaagcac gctggcactg tagacgtaga caaacacacg
     2041 ctgcggattg ggaatgatgt agttcaggca tgagccaatt agcagaatag caatagatac
     2101 tgccacaccc gcaaccggta cgccgtgacg ggaaactttt gccattgccg ccggtaactg
     2161 acggtttttc gccagtgcgt agagcatacg tccgcaactg tacatgccgc tgttacagcc
     2221 agagagcgca gccgtcagca ccacaaagtt gataatgccc gccgctgcgg taataccgat
     2281 tttggcaaaa gtcagtacga acgggctgcc gttgctgcca atttcattcc acgggaagat
     2341 ggtgacgata acgaaaatcg cgcctacgta gaaaatcagg atccgccaca gcactttgcc
     2401 tacggcactg cgcagcgtca cctgcggatt cttcgcttca ccggcagtaa tgccaatcag
     2461 ctccacgccc tggtaggacg ccaccacaat acacagagcg gtcaggaacc ctttccagcc
     2521 acccgcaaag aaaccgccat gctctgtgag attgctaaaa ccaatcgact gcccgccatt
     2581 gccaaagcca aagaaaatca cgcccaggcc aatgacaatc atcacgataa tcgtggtgac
     2641 tttgatcatc gcgaaccaga actcgatttc gccgtacaac cgtaccgccg ccagattcgc
     2701 caacgccacc agcgccaccg cgatcaatgc gggtatccac tgcgccatct ccggggacca
     2761 aaactggaca taaacgccaa tggcggtgat ttctgagatc cccaccgcca tccacataaa
     2821 ccagtaagac caggcggtga gatagccaaa aaacgggctc atataacgat gcgcataaac
     2881 ggcgaacgaa ccggtaaccg gttcgaggaa caacatttcg cccattgaac gcatgatgaa
     2941 aaaaacgaac agcccggcga tgatataggc caacaatacg gatggcccgg cccatttcag
     3001 ggtactggcg gcccccataa acaggccgac gccaatggtg ccccccaggg cgatgagttc
     3061 gatatgtcga gcttccagcc cacgctgtag ctctggtttg ttatctgcca taaatcctcg
     3121 tgttgtgttt gcatgctttc cggtgttacc ggttatcgtt atgggtacat cgagtgttgc
     3181 gaatgttttc gtaattcagg agaaatggca aataaagcat taaaaatttg aatgctttgt
     3241 gtaataaaaa agcagacagg cgacgaagtg accactccgt cgctttacaa agagaggaaa
     3301 atcagaggtt gccggtgtag tgccagcgta aataacgcag caaacgaagc tgacgcttaa
     3361 tgcggctcgg ctgcgaaagc aggcggtaga gccactccag tcccagcgtt tgccagattt
     3421 tcggtgcgcg ttttacgtga ccggtgaaaa catcgtaagt cccgccaacg cccatataca
     3481 gcgcatctgg atgcaccaga cggcagtcgc gcatgaagat ctcctgcttt ggcgatccca
     3541 tcgcaacggt gacgatttgc gctccgctgg catgaatgcg ttcaaacagc gcctgacgct
     3601 gctcgggttt aaaataacca tcctgactgc caacgatatt cacattccat tggttgcgca
     3661 gtttagcttc agtttgcgcc agcacttcag gtttaccgcc cacaagaaat accggcgtcc
     3721 cttctttgcc tgcgcgcgcc atcagctctt cccagagatc ggcaccggca acgcgggaaa
     3781 cctgcgcctg cgggtacttt ttacgtactg aacgtacaat gctgatgcca tccgcatatt
     3841 taaattcggc agcgttaatt aactccctga cctcggcgtt atcttcaatg gtcagcattt
     3901 tttcggcatt aatggcaacc agcgttccct gcttaagctg cccgtcagca aacagataat
     3961 cgagggcatg ctgcatatca cgccaaccaa tcaactgtaa gccacgcagc gtataggtcg
     4021 gtgccgtggt gttgttattc attgttatcc ttcaacctgc gtccggagcg atgattttgt
     4081 acgtttatga atgagtccgg cgctttcaaa aagccagtac aacagttttg cgatcatcag
     4141 acatgcgccg aagaccacga taaaaaagac tacgcgtgag acaaacgaat ccagcccttc
     4201 acgtgccagc acgatcatat tgaagatcgc cccaaagcag aaactgtgca atatcgcagc
     4261 cttatagcga ttagtctcgc ggttgcccag ctcatacagc cagtcgaacc atttgatgat
     4321 cagtccaacc acaatcgccc cgagtgggat gaacaacgcg ccgcccatca ccaccagtga
     4381 gcctataagc gtaggcgaga tcgccagtcc ggagtggtta ttcagtactt cccaggtaaa
     4441 gtaattggct gagttcagca ccatactcgg gcgccccggc cacagccagg aagggataaa
     4501 gacatagaaa tcgcggacaa ttggagccag gccctggaag tcgatgttgt cgtagttctg
     4561 caacagcaac gccagattct cccacggtga aaaggtgtcg cgagtgagat agagaaacgt
     4621 atagaacgct tcatcgccgc tcacattcat tccatagcgt tttaacgcca gccagaacat
     4681 gccaacaatc cccagcacgc ccgccgccgc cagcatccac aacgaaatcc agccgcgaat
     4741 aatgccaata aacaggaaga tagcgaatgc gatgatgata ttggcgcgag tgccgccgac
     4801 aatcatataa gtcagcaagc caaaggcgac cgtgctgacg aggaaaaaca gccacgcttt
     4861 gctgtcctgg cgcagaaagt agaccaccag catcgccggg atgaaaaagt aaaagaagcg
     4921 ttttaacgcc acgccggaga cttcactgga aaagatctga ctgtaggagt taagccggaa
     4981 cagcaaaaag ccgttgtgca tgaagaagat gccgacgctt accagcgcga tacccatcag
     5041 gatcacccac gtaagattgg tctccacgcg gttcatggta aacagcggac ggcgcggtac
     5101 atcagcaacg cgtttgcgta ggcgggtttt gtaggtgaca tagtaaaccg cgtagaagca
     5161 gcccgcagaa agcaacacct gcaacaagat ttctggcggc gcgacaccaa catcaaagcg
     5221 aaataccagc acgctggtca gcgggaagcc gaagaaaaag gtgagcaaaa acagcaatga
     5281 aaagaagaca ttgaagttaa agcgcacacg gcgaaactca aaccaggtca gcgtggcaat
     5341 aaacagcgtg cagagcagcc agaccacaaa caggccactg aattgcagca gactcatgcg
     5401 acctccccgg cggcaatcgc caacgcccgc tgccagcctt gtagatagtt agggctaaag
     5461 aaggcaatgg tgtttttatc cgccgacgcc aactggcgct gcgcttcacg cacaatatcc
     5521 tcgttgagat cgtcggtagt aaacagcact ggcaaatgct gttccgtcat atcctgccag
     5581 aacggatttt ccctgttaag cacacaagga atgcccgcct gaatcagtaa gcaaagcgta
     5641 ccaatgccct gctggcgggc aaaaataaag taaccaagat cgcactgacg aagtagcgcc
     5701 agataggcgt caaattccag tttttcgctc aaaatttgca gattttcttc gctgaataac
     5761 tccagccccg cctgacgaac ttcctcaatg tacgcttcgt tattaggcgg atatcccatc
     5821 ggcaccacca cttttaccgt atcgccaaat tgctgatgaa cggcacgtaa agcggcgaca
     5881 tgctcattgc tgcggtcgcc ggagttcccc accagaatgg tcatttttcc ttcacgttgc
     5941 cgatcgttcg ccatcgtatt gagagaaggg tccatccgcg tcgggaagta cagcagttct
     6001 ccccgcacct ttgggtgtgt tttggcaaaa aagctcaaat cgccgcgggt ggcaaataca
     6061 cagccgactc gcttttgcgc caggcgacgt agtgggtaaa aaagcttata tctcaagcca
     6121 ctggaaagct catacaggtc tgccccccag atatgccaga aaaactggct gggcttaatg
     6181 ccaccactca gcagagccag ccacagtgtg ggattgaact gaccgtggaa aaaaaatcgc
     6241 tgctgacggt tagcttttgc tttcgcgatg accgcttccg ccagcgattt tttcccaggg
     6301 aaaaattgca cagaaagcgc cggacagcta tcacttaagc cgtcgtcctt gccaacaacc
     6361 ataaactcgc gcgcgtgctc gctcgtcgcg gccagcgcgt cattgaaaaa ccgcaaaacg
     6421 gttcggttat ggtgagggat atccgatccc agtacgtgaa tcagtacagt catgcccgcc
     6481 tacgccagag taaaaacacg ccacaacaaa gagaaaaata gacgatataa gttgccatat
     6541 atgcctgcgc cgcgcccagt gcaccatgcg cagggattag ccagtgggca aataccatca
     6601 ataaagtgaa ctggctgact tccgccagaa tataaaaccg cagtgacgct ttggcgatca
     6661 ccagataacc aaagacataa gcgcccactt ttaacacatc acccactaac tgccaggcaa
     6721 agagatcgcg catagcggta aatttattcg acaacagcaa ccagatagca aaatcacgca
     6781 gcaaccagac ggtaaaactc gctgccgcca ctgccggtaa gacgaatttc agcgatttaa
     6841 ccacttcccg ggtgatatcg cgcttttccg ttagccgcga caacgtgggc agcaaatata
     6901 cgctgaacga tgccgtaata aattgcaggt aggcatcgga aatactgctc accccttgcc
     6961 agatccccac ttcatcccag ctatactgcg ccgccagcag tttacgcatc atgatgtaag
     7021 caacaggcaa ggtcaccgac gtaatcaacg ccatgagcgt aaatttgctc aactgccctg
     7081 ccagaccgtt atcccagctg ggtttcagat agcttaacgg gatgacacca cgtttaatca
     7141 acatgatggc ggcaggaatt accaccagcg cgggaatcag cgccagaccc agcaacgccc
     7201 cttcataacc gcccaaacgg taactgacgt agtacgcgag aacgccaatc aagctgccga
     7261 caatcagcga taacgcatta cctgcggcat cgcgaaagcc tttcatcagc gccagtaaca
     7321 ggttgcccca ggcgatcccc atttgcacca gcgccactaa acgcaccagc ccctgatagt
     7381 cggtattacc aaacaatccc tggctgattg gcgcagctgc cagcacaaaa accagcgcca
     7441 tcagcgtaga gaaaccaagt accatcgctg atgaagtgcc gaccacgcgg cgcagctgtt
     7501 gcggattatc atggtactgg gcaacgtatt tggttacacc gttaaagatg ccagccccgg
     7561 caagcacgcc gagcacggta atcaactggc ggaaatttgc cgccagccca agccccgccg
     7621 gaccaaatga caccgccagc aacttaccga ccagtaaccc ggcaccaatc ttgaccagtg
     7681 tactggccgc cgtccacaag gacgcttttg ccaacgacat atcaggagaa gtagttcaac
     7741 aaagtcgcaa ttaccgtacg ctgattgacg ggcgacaggt tgtagaacag cggcaggcgc
     7801 agcaggcgct cgctctcttt ggtggtgtag cgatcttcac cgtggaactc accaaagtgt
     7861 tcccccgcag ggcaaccgtg cagcggaatg taatgaaaca ccgccatgat ttccgcttct
     7921 ttcagaaagt taatcaacgc gctccggtca tcaatatccc gcagtttaat gtagaacata
     7981 tgcgcgttct gcacgcagcc atcgggaatc gacggcagct cgatacgccc ggctttcgcc
     8041 agaggcgcta acgtatcgta gtagttttgc cacagcgcca gacgttgctg gttgatacga
     8101 tccgctgctt ccagttgcgc ccacaggtac gcagcttgca gatcggacat caaatagctg
     8161 gagccaatat cgcgccaggt atatttatcg acctgaccac ggaagaactg gctgcggtta
     8221 gtgccctttt cacggatgat ctcggctcgt tcgattaacg ctttatcgtt aatcagcgtc
     8281 gcgccgcctt caccacccgc cgtgtagttt ttggtttcat ggaagctaaa gcagccaata
     8341 tgaccaatgg ttcccagtgc acgccctttg taagtggaca tcacgccctg agcggcatct
     8401 tccaccacaa acaaattatg ctttttcgcc aacgccataa tggtgtccat ttcgcaggcc
     8461 acacccgcgt aatggaccgg cacgataacg cgcgttttgt cggtgatcgc tgcttcaatc
     8521 aacgtttcgt cgatgttcat ggtgtccggg cgaacatcca caaagacgat tttcgcgcca
     8581 cgcagcacaa aggcattggc ggtggagaca aaggtgtagc tcggcatgat cacttcatcg
     8641 ccaggctgga tatcgagcag cagcgccgcc atctccagcg aagcggtgca ggacggcgtc
     8701 agtaacactt tggcgctgcc aaaacgttgc tccagccact gctggcagcg acgggtaaaa
     8761 ccgccatcgc cacacagttt gccgctaccc attgccgact gcatatagtc gagttcggtt
     8821 cccaccaccg gcggtgcgtt aaatggaatc atgtgatcac ctgtataacc agtacgcggt
     8881 gctttctaca ttcgcaccac tttgtatgta tcgtttaagc gcggcggtgt tgcccatttg
     8941 ggtcgccacc cgcaaagttg ttttaccgcg agcatacgcc cagtttagcg ccgtttgcat
     9001 cagctcagca cctgcaccgc gtccagccag caggccaatt cgcgcatctg tcgcattgag
     9061 ttcccgtaaa gagacatagc cgcgaatatc gccggacgcc gcacgtagaa tcagacattg
     9121 atgatcaaag gtgccgcgca cggcattttc aatccactgt gcataaaagc gaccgctggc
     9181 gtcaggcgca taccacggcg cacgaaaacg gctttgcgca aatgcggcgc tagctaactg
     9241 acgtaatgcg ggaatatcgg tctcttgtgc cacgacagca ccgctatcac tgacattgtt
     9301 cacgggtagc gccaaatcaa cttcaccttc taccagggag aatcccagct gttgcagggc
     9361 atccagttcc cccgtatttg atgccgcaat tttggcctgc acccgtgacc atggcgctaa
     9421 ggcgtctgac gtcaggagcg gtgcttcaga cgtaatgcgc acgatggcgc tgttaacacc
     9481 aaagaaggcg ttttcccagg ttagtggctc aatactggcg cggacgggca cgaagtaact
     9541 ccagcagata ttggccgtag ccagttttcg ctaatgaact ggcagcacgc ttcacaccct
     9601 cgtcatcgag ccagccgtta cgccaggcga tctcttccag gcaggcaatc ttaaagccct
     9661 ggcgtttttc tactgtctgc acaaaagtgc tggcctcaat caggctgtcg tgagtgccgg
     9721 tatccagcca ggcaaatccg cgcccgagca gttcaacggt caggttgccc gcctcgaggt
     9781 acatctggtt gatggaggta atctccagtt caccgcgctc cgacggcttc acctgctttg
     9841 cgtactccac gactttactg tcgtagaaat aaagcccggt caccgcccag tttgacttcg
     9901 gctgttttgg cttttcttca agcgagatag cgcggaaatt gtcgtcaaat tccaccacgc
     9961 caaagcgttc cgggtccatc acctgatagc caaaaaccgt cgccccttcc gtgcgcgccg
    10021 caacatgacg cagcttcgga ctgaagccct gaccgaagaa gatgttatcg cccagcacca
    10081 gacaagaagg ttcaccatta aggaaggttt caccgatgat aaaggcctgc gccagaccgt
    10141 ccgggctggg ctgttcggca tattccagct ggataccgaa ctcactacca tcgcccagca
    10201 ggcgctggaa ataaccttta tcttccggcg tagtgatgat gagaatttcg cggataccgg
    10261 ccagcatcag caccgacagc ggatagtaaa tcattggctt atcgtaaatc ggcaacagtt
    10321 gcttcgatac gccgcgcgta atcggatgca atcgggtacc ggaaccgccc gccaggataa
    10381 tacccttcat gtttgcctcc gctgaaaatt agcctttcag acctaaacgc tcaccctgat
    10441 agctgccgtc ctgcacctgc ttccaccagc tttcattagc cagataccac tgaaccgttt
    10501 tacgcattcc actttcaaag gtttcctgcg gcagccagcc aagttcacgg gcaattttcg
    10561 aagcatcaat ggcataacgc agatcatgcc ccggacggtc agcgacaaag gtgatcaggt
    10621 cacgatagta cgccacgccg tgcggcttgt tcgaagccag ttcttccagc agctcgcaaa
    10681 tggtttccac aacatcgaga ttcttacgct cgttgtgacc accaatatta taggtttcac
    10741 cgactttccc ggtggtcgcc acgcaataca gcgcgcgggc gtgatcttcc acatacagcc
    10801 agtcacggat ttgctgcccg ttgccatata ccggcagcgg tttacccgcc agcgcgttga
    10861 ggatcatcag cgggatcagt ttttccggaa agtggtaagg accgtagtta ttcgagcagt
    10921 tggtgatcag cgtcggcaga ccgtaggtcc gcagccaggc gcgcaccaga tggtcgctgc
    10981 tggcttttga cgcggaataa gggctgctcg gcgcatacgg cgtggtttcg gtgaagaaat
    11041 catccgtcga gtgcaggtca ccatatactt cgtcggtgga gatatgatga aaacggaacg
    11101 ctgatttttt atcttccgtc agcgtattcc agtaagcccg cgccgcttca agcaatgtat
    11161 aagtcccgac aatgttggtt tcaataaacg ctgccgggcc gtcaatagaa cggtcaacat
    11221 ggctttcggc tgccagatgc atgacacagt ctggctgatg ctcagtgaat acgcgtgcca
    11281 gttctgcccg atcgcagata tcaactttct caaaggcaaa gcgctcgctt tgcgcgaccg
    11341 gtgccagcga catcaggttt ccggcgtagg tcagcttatc gaccactacc accgcatcgc
    11401 tcgtttcgtt gatgatataa cgcaccagcg ccgagccaat aaacccggca ccacctgtta
    11461 tcagaatttt tctcatcagc gccagactcc tttggcatcg acgacatact gctgatggac
    11521 attgtcgcca ttgataactt tgaactgact atgatcgacc agcatcacca gcacgtctgc
    11581 cgttgccagc gcctcgtcaa gctgcgccag agtacaaagc ccggtcagtt ttttcggcaa
    11641 ctggtggatg ttaggctcaa caaccagcgt ttcgccgctg tgccactggg cgatcagttc
    11701 agcgatttcc atcgccgggc tttcgcgcag gtcatcaata ttcggtttaa acgccagacc
    11761 aaagcaggcg attttcagtt cactggctcg tttatcggta gcagcaaggc agtcggcaac
    11821 cgccgctttc acctgatcga taacccagaa cggtttgtga tcgttaactt cgcgcgcggt
    11881 acggataagc cgcgcctgct gggggttctg tgccacgata aaccacggat caacagcaat
    11941 gcagtgaccg cccacgccag ggccaggctg aagaatatta acgcgagggt gacgattcgc
    12001 caggcgaatc agttcccaga cgttaatccc ctgatcggca caaatcagcg acaattcatt
    12061 agcaaaagcg atattcacat cgcggaagct gttttcggtg agcttacaca tttccgccgt
    12121 ccgcgagtta gtgacgacac actcaccttc gaggaaaatt ttgtacaatt cgctggcgcg
    12181 ggccgaacaa accggcgtca taccaccaat cacgcgatcg tttttaatca gctcgaccat
    12241 aacctggccc ggtaatacac gttccgggca gtaagcaatg ttgacgtccg cctgctcgcc
    12301 cacctgctgt gggaaagtca gatccgggcg catctctgct aaccattctg ccatcttctc
    12361 ggttgacccc accggcgagg tggattcaag gatcaccagc gcgccttttt tcagcactgg
    12421 cgcaatggag cgagcagccg attcaacgta ggtcatatct ggctcatgat cgcctttaaa
    12481 cggcgtaggt acagcaatca gccaggcatc cgcttcaact ggcgtcgtgc tcgctcgtaa
    12541 aaaaccgcct tctacggcag tttttactac gctcgccaaa tcaggctcga cgatatggat
    12601 ttcgccacga ttgatggtat caaccgcgtg ttggttgata tcgacaccaa tcacctgttt
    12661 ttgccgtgag gcaaacgctg ctgctgtagg cagcccgatg taacccagtc cgataacaga
    12721 aatggtcgca aaactcatag tgatatccga ttatttttta acgcttccag aatgcgagag
    12781 catgcctgac catcaccata cgggttatgg gcgcggctca tagcttgata ttcgttttcg
    12841 tcttttaaaa gacgcgtcac ttcctcgaca attcgctgct tatccgtgcc taccagacgc
    12901 accgtacccg ccgtcaccgc ttccggacgc tcagtggtat cgcgcatcac cagcaccggt
    12961 ttccccagcg aaggcgcttc ttcctgaatg ccgcctgagt cagtcaaaat cagccaggcg
    13021 tggttcatca gccagacaaa cggtaaatac tcctggggat cgatcagaat gacatttttc
    13081 acatgcccca gaatgcgatt gaccggctct ctgacgttcg ggttgagatg caccggatag
    13141 acaatctgga tgtcctggtg cgtggtggcg atgtctgcca gcgcgtggca gatttcttca
    13201 aagccacgac cgaaactctc acgcctatga ccggtcacca gaatcatctt tttatcgggg
    13261 gcgataaacg ggtaatttgc cgccagttct gaacgcagcg tgtcgctgct catcacctgg
    13321 tcacgcaccc ataacagtgc atcaatgact gtattaccgg taatgaagat tcggctatcc
    13381 gcaacgtttt cacgcagcaa gttttgccgg gaagtttcgg ttggagagaa gtgatacatc
    13441 gccagatgcc cggtcaatgt acggttagcc tcttccggcc acggcgaata gagagcgccc
    13501 gtgcgcagac cagcctcaac gtgaccaaca ggaatacgct gataaaacgc cgccaggctg
    13561 gttgccagcg tcgtcgtcgt atcgccgtga accagcacga cgtctggttt gaactcggca
    13621 agaataggtt ttagcccttc cagaatccga caggttatct ctgtcaggcc ctgtcctggc
    13681 tgcattatgt tgagatcgta gtcaggtaca atggaaaaga gtttcagtac ctgatcaagc
    13741 atctcccgat gctgcgcagt gacgcaaact ttagcctcaa aaaaaggatc ttttgccaac
    13801 gcatgcacca acggagccat cttgatggct tccgggcgcg taccaaatac agtcagtact
    13861 ttcacatcga ttctcttcga ataagcggcg agcgcctttg cgctcaccgc agcagtgttg
    13921 ctatttcgag caacggcggg ttaatgcgac accagccccg atcagccccc cgacaatgcc
    13981 ccacataatc atcaggaagg cacgacgtgg gctatcgcgt tttaccggtt cttccggcgt
    14041 acgcaaatag cgataggtct gaaaacgcgg atccagggtt ggaccaacat tcagggtgtt
    14101 taacatggct cgattctgat catagtcgag atcaaaggcc ggaccgacgg cctgtaaatt
    14161 ttccagtcga gcctggagca ttggacgccc aagcaggaac atttctgaat caggtaattc
    14221 ctcggcaggc acatctgtcg cactgcgcga aatattatgc tgctcagcaa ttttcagcgc
    14281 ctgctcaata ctgttcatcc ggcggtcgta gatggctttc gccacctctt cctgacgctt
    14341 cacctgagct ttcatctgga tggtgcgtgc cgcccatgcg cctttcagct catcattcag
    14401 atggctggct gcacgctggc tggcaaaagc aacatactga cgtaacaggt tattagcgtc
    14461 aggcgcggtt tcggcaataa gcttcacgct gtcattgacc gcgcgagtaa agtctccggg
    14521 gataaactgg atgttgttaa tcatttcatc cagcaacgcc gcatcggctt tgctgttgcc
    14581 caccatccgc tgtttgtaat agtcggtttg cagccagaac tctctgcggg tatcccacga
    14641 ggccagttgc ataacaaact ctttataggc ttcgtccatg accgatggtt gatcggcaga
    14701 agccatgttt gaacggacat ccaggttacg caaaaattgc tgctgcgagt aatatccccc
    14761 cagcatattc accgttggac gatcggtaat cgccgtcgag ctccactcct gacgagcaaa
    14821 aaaagtatac gccagcgcga ttaacgcaaa cgccagcccc atgccaataa tccatagctt
    14881 cccagcccac aaggtacgaa acaacccacg aatatccagt tcattttcag cgtcttcggc
    14941 cggtttccca ggcattggtt gtgtcatcac atcctcattt atttggttaa attggggctg
    15001 ccaccacgat ttctacgcag tctgcgtttt acgcgcttaa taaagcgagc aactttccag
    15061 gcacgcttaa tgcaatatcc atagaggaag aatgctagca aaaagagcac cagcatgacc
    15121 cactccggga caaaatgaga atattctgcc agcacgccaa tggaagcgag cagtgctgcg
    15181 gcaagggtaa tcagcacaaa cgcctgacgg gaagtaaacc cggcacgcat gatcaaatgg
    15241 tgaatatgct gacggtcagg agagaatggg ctcatgcctt tacgcaggcg acggtacata
    15301 atcgccacca tatccattag cggaatggcg attatccaca aagcggtaac cgggctgatg
    15361 ggatgggttt tgccctgggt cgtttcgagc aggatccaga taacggtaaa accaatcagc
    15421 gtactgcccg catcacccat aaagactttg tagcggcgac ccaggatacc aaggttaagc
    15481 atgatgtatg gcaggatggc ggcgatcatc gcaaagcacc agattgcgag gctggtttgc
    15541 ccgtcgaacc acaaaatcat accgattgct gcaaacgaga cgcaggacaa cccgcccagc
    15601 aagccatcaa tgccatcaac catgttgaac gcattaatgg ccgcccagac ggcaaatagc
    15661 gtcaggaagt aaccaaacgg tccgagcacc atctcccagg agccaaagat ataacccagg
    15721 ctactgagat aaagtttgcc gaacaccatc ataacaatgc caacagcggc ctgtatggtg
    15781 gcacggattt ttacgctgat atcaaaacga tcatccagcg cgccaatgaa aacaagcaca
    15841 ccggcacaag cgagatagag agatgcatgc ggaatatagt aatcgacaat tccgaacgtg
    15901 aagcaaatcc ctgcgtaaac cgaaatcccc ccaacgagag gtatcaatcc ctggtgacgt
    15961 ttgcggaagt ttggtttatc cactaaaccg actttttttg ccaccttacg ggcaaaaaac
    16021 agaaacagtg tcgtgaataa aaaaatactg atgagatcag tactcactgt cagtaaattc
    16081 acaatgcatg ctctcagaga aaattattag cagaagtata accacgaaga cctttattca
    16141 gaagggaaag tccgttccga acccaccagc cctgtgcaaa aatcaaccat ttgatatgca
    16201 aattgcataa catgccagca aattccagga attttcctaa catcgccagc gcgacattaa
    16261 gattacagat ataaaagcaa aacgccacgt aaacacgtgg cgtttttggc ataagacaaa
    16321 tttatgagcg tttcatcatt tcgaagaaat cgtcattggt cttggtcatg gccagtttat
    16381 taatgaggaa ttccattgca tcgatttcgc ccatcgggtg aatgattttg cgcaggatcc
    16441 acattttctg cagttcttcc tgagtcgtga gcagctcttc tttacgggta ccggaacggt
    16501 tgtagtcgat agccgggaag acgcgttttt cagcgatctt acgagagagg tgcagttcca
    16561 tgttgcctgt acctttaaac tcttcgtaaa taacttcgtc cattttagaa ccggtatcga
    16621 taagcgccgt cgcgataatg gtcaggctgc cgccctcttc cacgttacgc gccgcaccaa
    16681 agaagcgttt cggacgatga agggcattgg cgtccacacc accggtcaac actttacctg
    16741 acgccggaac aacagtgttg taagcgcgcg ccagacgagt gatggagtcg agcagaatga
    16801 taacgtcttt cttgtgctca accaggcgtt tggccttctc gatcaccatt tccgcaacct
    16861 gaacgtggcg agatgcgggt tcgtcaaagg tagaagcaac aacttcacct tttaccagac
    16921 gctgcatctc ggttacttct tccggacgtt cgtcgatcag cagaaccatc agcacacaat
    16981 ccgggtggtt gtaagcaatg ctctgagcaa tgttctgcag cagcatagtt ttaccggctt
    17041 tcggcggtgc cacaatcaga ccacgctgac cacgaccgat aggtgatgcc agatccagta
    17101 cgcgagcagt taaatcttca gtagaaccgt taccacgttc catacgcaga cgagagtttg
    17161 cgtgcagcgg ggttaagttc tcaaagagga ttttgttgcg ggcgttttca ggtttgtcga
    17221 agttaacttc gttaactttc agcagcgcaa aatagcgttc accttctttc ggcgggcgaa
    17281 tcttaccaga gatggtatca ccagtgcgga ggttgaaacg gcggatttgg ctaggggaaa
    17341 cgtagatgtc atcaggaccg gcgaggtagg agctgtctgc ggaacggagg aaaccaaatc
    17401 catcctgcaa tatctccagt acgccatcac caaagatatc ttcgccactc tttgcgtgct
    17461 gcttcaggat ggcaaaaata atgtcctgct tacgcatgcg agccaggttt tccagcccca
    17521 tattttcgcc gagagtgatc agctcagaaa ccggcgtatt ctttaattcg gtaagattca
    17581 tagtggtgtg agttcttaaa cttggggtaa atctcgaact taatgttgtg aatggtatgg
    17641 caggatcatc catgcctgtt tacggtcatc gactcatgtc ttttcgctgc ctggtcacag
    17701 gagagtacgc agaactgaaa cgacaagacg gattgagtga cgagccagaa atctgttcac
    17761 ttcgcattta agataaaacg ggaagcgttg gaaattacaa gattcaaact taataaggta
    17821 tgtttaatac gaagtcaaca ctaagttagc atgactcacg ccgggcgtcc agtttttagc
    17881 gacggggcac ccgaacatga aattccctta cgccaggtta gcgtcgagga actctttcaa
    17941 ctgaccttta gacagtgcgc ccactttggt tgccgccact tcaccgtttt tgaacagcag
    18001 cagagtcggg ataccacgga tgccatattt cggcgcagtg ccagggtttt gatcgatgtt
    18061 cagttttgca acggtcagtt tgccctgata ttcgtcagcg atttcatcca gaatcggggc
    18121 gatcattttg cacggaccgc accactctgc ccagaaatcg acgaggatcg ccccgtccgc
    18181 tttgagtaca tccgtgtcaa aactgtcgtc agtcaggtga ataattttat cgctcatata
    18241 taactccaca ggaataagcc tggcgtgttg gtttcgttgt tggtgtaaca ttaaccaact
    18301 aaaggttgac tttatttcac cggatacgct ttcgtaaagc aatagtaagc tgatattcta
    18361 ccacactatg agcaaaacac atttaacaga acagaagttt tccgacttcg ccctgcatcc
    18421 gaaggttgta gaagcccttg aaaaaaaagg gtttcataac tgtacgccca ttcaggcact
    18481 ggcccttccg ctgacgctgg cgggtcgtga cgtagccggg caggcgcaaa ccggtaccgg
    18541 gaaaacgatg gcgtttctta cgtcaacgtt tcattatctt ctctctcatc ctgcgattgc
    18601 cgatcgcaag gtgaatcaac cgcgtgcctt aattatggca ccgacgcgtg aacttgccgt
    18661 gcagatccac gccgacgcgg aaccgctggc ggaagctacc ggcctgaagc tgggcctggc
    18721 ttacggtggt gatggctatg acaaacagct gaaagtgctg gaaagcggcg ttgacattct
    18781 gattggcacc acgggtcgtt taattgacta cgccaagcag aaccacatta acctcggtgc
    18841 cattcaggta gtggtactgg acgaagccga tcgcatgtac gatctaggct ttattaaaga
    18901 tatccgctgg ctgttccgcc gtatgccgcc tgcaaatcag cgcctcaaca tgctgttctc
    18961 cgccacgctt tcgtaccggg tacgtgaact ggcgttcgag cagatgaaca atgccgaata
    19021 tattgaagtg gaaccggaac agaaaacggg ccaccgtatt aaagaagagc ttttctaccc
    19081 ttctaacgaa gaaaaaatgc gtttgctgca aacgctgatc gaagaagagt ggccagacag
    19141 agcgattatt ttcgccaata ccaaacaccg ttgtgaagag atctggggcc acctggcggc
    19201 agatggtcat cgtgtcggtt tgttgacagg cgatgtcgcg cagaaaaaac gtctgcgtat
    19261 tcttgatgaa tttacccgtg gcgatctgga tattctggtt gccaccgacg ttgccgcgcg
    19321 tggtttgcat attccggcag tgacgcacgt ctttaactac gatttacccg atgactgtga
    19381 agattacgtt caccgtattg gtcgtacagg tcgcgcaggc gcaagcggtc actctatcag
    19441 cctggcgtgt gaagagtatg cattgaattt gcctgctatt gagacctata ttggtcactc
    19501 aattccggta agcaaataca atccggacgc attgatgacc gatctgccaa aaccgctgcg
    19561 cctcacgcgc ccgcgcacag gcaatggtcc gcgtcgtact ggcgctccgc gtaatcgtcg
    19621 tcgttcaggt taaataaaat gtcatactca aaatcattca aactgtatca agatggcaag
    19681 agaatgaatc ccgatgagct tactgtagta agtgattcgg gtgaaagaat gcagccaaca
    19741 cagagacaga ttgaaggatg aagagtatgg gttccacctc gtcgctgtat gcagccattg
    19801 atctcggttc gaatagtttt catatgctgg ttgtgcgcga ggtggctgga agcatccaga
    19861 cgctgacgcg aattaaacgc aaagtgcgtc tggctgctgg cctgaatagc gaaaatgacc
    19921 tctctaatga agcaatggag cgcggttggc aatgtctgcg cctgtttgct gaacgtctgc
    19981 aagatatccc tccctcgcaa attcgcgttg tcgctacggc gacattacga cttgccgtca
    20041 atgcgggtga ttttattgcc aaagcgcagg aaatcctcgg ttgtccggta caggtgatca
    20101 gcggtgaaga ggaagcgcgt ctgatttatc agggcgttgc tcacaccact ggtggtgccg
    20161 atcagcgcct ggtggtggat ataggcggtg ccagtactga actggtaacc ggcacgggtg
    20221 cacaaaccac ctcgttgttc agcctgtcga tgggctgcgt cacctggctg gaacgctatt
    20281 ttgccgatcg taatctgggg caagaaaatt ttgatgctgc agaaaaagcg gcacgcgaag
    20341 tgttacgtcc ggttgccgat gaattacggt atcacggctg gaaagtgtgc gttggtgctt
    20401 ccggcaccgt gcaggcgtta caggaaatca tgatggcaca ggggatggat gaacgcatta
    20461 ccctggaaaa gttgcagcaa ttgaaacagc gagccattca ttgcggtcgg ctggaggaac
    20521 tggagattga cgggctgacg ctggaacgtg cattagtgtt cccgagtggt ctggcgatcc
    20581 tgatcgccat ttttaccgaa ctgaatattc agtgtatgac cctggcgggc ggtgcgctgc
    20641 gtgaaggcct ggtctacggc atgttgcatc tggccgtcga gcaggatatt cgcagccgta
    20701 cactgcgtaa tattcaacgc cgctttatga tcgatattga tcaggcacag cgcgtagcca
    20761 aagttgcggc taacttcttc gatcaggtgg aaaacgaatg gcatcttgaa gcaataagcc
    20821 gcgatttgct catcagcgcc tgtcagcttc atgaaatcgg cctgagcgtt gacttcaaac
    20881 aagctccgca acacgctgct tatctggtgc gtaacctgga tcttcccggt tttacccccg
    20941 cacagaagaa actgctggcg acgctactgc tcaaccagac taacccggtc gatctctcat
    21001 cgctgcatca gcaaaatgcc gtaccaccgc gcgtcgcaga acaactctgc cgtttactcc
    21061 gcctggctat catttttgcc agccgtcgcc gtgacgatct cgtgccagag atgacattac
    21121 aggctaacca tgaactgttg accttgacgc ttccgcaagg ttggctaacc caacatccgc
    21181 tgggtaaaga gattattgct caggaaaacc agtggcagag ctatgtccac tggccgctgg
    21241 aagtgcatta atgagtaagt gccggatgcg atgctgacgc atcttctccg gccttgatta
    21301 tttccctcgt tttgccgcca tcatcgcttt cagattcgcc agatggcttt gccctttctg
    21361 catccgttct tcggcactga ccactttgcg ctcctgttcc caaatcagat catcctgcgg
    21421 cagctccagc aaaaagcggc tcggctccgg gcgcaccagt tcgccgtact gacggcgttc
    21481 tttacacagc gtaaaggtca attccttctg ggcgcgggta atgccgacat aggccagccg
    21541 ccgctcctca tcgatattat cttcatcgat actgctctgg tgcggcaaaa acccttcttc
    21601 cataccgacc atgtagacat aaggaaactc cagccctttt gacgcgtgga gagtcatcag
    21661 ttgcacctga tccagctctt cttcactctc accacgctcc atcatgtcgc gtaaagtaaa
    21721 gcgcgtcacc acctgggtga gcgtcatcgg ctcatccagt tcgctgcctt ccagcatctc
    21781 cgtcatccag ctaaacagtt ggttgacgtt cttcatgcgc atttcggcag ctttcgtgct
    21841 gggcgatgtt tcgtacagcc aggattcata atccatgccg tggatcagat cgcgcaccgc
    21901 ggcaatcggc tcccgctccg ccagacgctg gatttctgcc aaccagtgag tgaagcgggt
    21961 caatgcttca taaccacgtc cgctaagcgt ctgactcagg cccatatcaa agctggcggt
    22021 aaacatgctt ttattgcgcg tcatcgccca ctcacccagc tttttcagcg tcgccgggcc
    22081 aatctctcgc ttcggcgtgt taacgatacg cagaaatgcg ctgtcatcgt ccgggttagt
    22141 cagcacgcgc agataagcca gcaagtcctt gatttcagga cgagagaaaa acgacgtacc
    22201 accagaaatt ttgtacggga tgcggttttg catcaggaat ttttcaaaca cccgcgactg
    22261 atagttaccg cgataaagaa tagcgtaatc tttgtactgc gttttattga cgaagtgatg
    22321 cgcaatcagt tcaccagtga cgcgctcggc ttcatgttct tcgttattcg cgcttaaaac
    22381 ttttagctct gtgccgtaac ccagttcgga gaacagacgc ttttcaaaga cgtgcgggtt
    22441 attggcgatc aggatgttcg ccgctttcaa aatacgtccg gaagagcggt agttctgctc
    22501 aagcttaatc accttcagcg ccggaaaatc ctggctcagc agcaccaggt tttgcggacg
    22561 tgcgccgcgc caggagtaga tcgactggtc atcgtcaccc accacggtaa agcgcgcgcg
    22621 gctgcccacc agcagtttca ccagttcata ctggctggtg ttggtgtcct gatattcgtc
    22681 caccagcagg tagcgaattt tgttctgcca gcgctcgcgg acttcttcat tgcgttgcag
    22741 aagtagcgtc ggcaataaaa tcagatcatc gaagtcgagc acattacagg ctttcaggtg
    22801 tgcatcatac agcccataac aatgggcaaa aatacggtcc cgctcgccaa tcgcactggc
    22861 tgccgcctgg gacggtgttt tgagatcgtt cttccagtta gagatggtcg aaatcaactg
    22921 ttgcaggaga actttgtcat cttcaatcag cccttcggtc aactctttga gcaacgccag
    22981 ctgatcggta tcgtcaaaca gcgaaaagtt cgccttcatc cccagcgccg catactcacg
    23041 cttgatgata tccagcccca gcgtgtggaa ggttgagatc atcagcccat gcgcctcttt
    23101 gcgacccagc gtctgtccga cacgctcttt catctcgcgc gccgccttat tggtaaaggt
    23161 caccgccgcg atatgtcgcg cctgataacc gcacccgcgg atcagatggg cgattttatt
    23221 ggtgataaca cgagttttac cggaacccgc acccgccagc accaggcagg ggccggtaac
    23281 gaattcgaca gcttgttgtt ggccggggtt tagacgcata ggaaaatcgc tcaatgaaag
    23341 tcgggagggg aaaataacag cgtggtagta tagccagcct catccttacc actcaaggca
    23401 cgatcatggc aaaaacagca gcagcactgc atatccttgt aaaagaagag aaactggctc
    23461 tggatcttct cgtgcagatt aagaacggtg ccgatttcgg caagctggcg aagaaacact
    23521 ccatttgccc atcaggcaaa cgcggcggtg atttaggtga attccgccag ggtcagatgg
    23581 ttccggcgtt cgataaagtt gtgttctctt gtccggtact ggagccaacc ggcccgctgc
    23641 acacccagtt cggttaccac atcattaagg tgctgtaccg taactaatag caaggccttc
    23701 tccgggagaa ggccttgaac tctgaatcgc tattaacccg caacagcaat acgtttcata
    23761 tccgtcatat agccgcgcag tttcttacct acttgctcaa tcgcatggct gcgaatcgct
    23821 tcatttacat cacgcagctg cgcgttatct accgcacctt ccggaatagc tttacccaaa
    23881 tcgcccggtt gcagttctgc cataaacggt ttcagcagcg gcacacaagc gtaagagaac
    23941 agatagttac cgtactcggc agtatcagag ataaccacgt tcatttcata cagacgctta
    24001 cggacgatgg tgttggcaat cagcggcagt tcgtgcagtg attcatagta agcagattct
    24061 tcgatgatgc cggaatcaac catggtttcg aacgccagtt caacgcccgc tttcaccatc
    24121 gcgatcatca gtacgccttt atcgaagtac tcctgctcgc cgattttgcc ttcatactgc
    24181 ggcgcggttt cgaaggcggt tttgccggtc tcttcacgcc aggtcagcag tttcttatca
    24241 tcgttggccc agtccgccat cataccggag gagaattcgc cggagatgat gtcgtccata
    24301 tgtttctgga acagcggtgc catgatctct ttcagctgtt cagacaacgc gtaagcacgc
    24361 agtttcgccg ggttagagag acggtccatc atcagtgtga taccgccctg cttcagcgct
    24421 tcggtgatgg tttcccaacc gaactgaatt agtttttctg cgtatgccgg atcggtgccc
    24481 tcttccacca gcttgtcgaa gcacagcagc gagccagcct gcaacatacc gcacaggatg
    24541 gtttgctcac ccatcaggtc agatttcact tccgcaacga aggacgattc cagcacgccc
    24601 gcacggtgac caccggttgc tgccgcccat gctttagcga tcgccatgcc ttcgcctttc
    24661 ggatcgtttt ccgggtgaac ggcaatcagc gtcggtacgc cgaatccacg tttgtactct
    24721 tcacgcactt cggtgccagg gcatttcggc gcaaccatga ctacggtgat gtctttacgg
    24781 atctgctcgc ccacttcgac gatgttgaaa ccgtgcgagt aacccagcgc cgcgccgtct
    24841 ttcatcagtg gctgtacggt gcgcactaca tcagagtgct gcttgtccgg cgtcaggtta
    24901 accaccagat ccgcctgtgg gatcagttct tcgtaagtac ccactttaaa accattttcg
    24961 gtcgctttac gccaggacgc gcgcttctcg gcaatcgctt ctttacgcag agcgtaggag
    25021 atatcgagac cagaatcacg catgttcaga ccctggttca gaccttgtgc gccacagccg
    25081 acgatgacta cttttttacc ctgaaggtag ctcgcgccat cggcgaattc atcgcgcccc
    25141 ataaagcgac atttgcccag ctgtgccagc tgctgacgca gattcagtgt attgaagtag
    25201 ttagccatgg tgattcctcg tgatgttgtg cttcttattg ttcggttcgc gtttgcgaga
    25261 ttgaattcac tatatgacag gaaatttatt gcggaaattg atatattcac aacgtcacat
    25321 tgcaatttct gcaacgtcaa cattgagggc tgcgccattg gatttacgcg atctgaaaac
    25381 cttcctgcat ctggcggaaa gccgccattt tggccgcagc gcgcgggcga tgcacgttag
    25441 cccgtccacg ctctcacggc agattcagcg cctggaagaa gatctcggcc agccgctgtt
    25501 tgtgcgcgat aaccgcacgg tgacgctgac tgaagcgggc gaagaactgc gcgttttcgc
    25561 ccagcaaacg ctgttgcagt atcagcagct gcgccacacc atcgaccagc aagggccgtt
    25621 gctctatggc gaattacata tcttctgctc ggtgaccgcc gcttacagcc atctgccgcc
    25681 gattctggat cgcttccgcg cggaacaccc gtcggtggag atcaaactca ctaccggtga
    25741 tgcggcagat gcaatggaaa aagtcgtcac aggtgaagct gatctggcga ttgccgggaa
    25801 gccagagact ctgcccggcg cggtggcgtt ttcgatgttg gagaatctgg cagtggtgtt
    25861 gattgcaccc gcattgccct gcccggtgcg taatcaggtt tctgtcgaaa agccggactg
    25921 gtcaacggta ccgtttatca tggccaatca ggggccggtg cgccgccgca ttgagttgtg
    25981 gttccgacgc aataaaatca gtaacccgat gatttacgcc acggttggcg ggcatgaagc
    26041 gatggtatcg atggtggcgc tcggctgtgg cgtggcattg ttgccggaag tggtgctgga
    26101 aaacagcccc gaaccggtgc gtaaccgcgt gatgatttta gagcgcagcg atgagaaaac
    26161 gccgtttgag ctgggcgtgt gtgcgcagaa aaagcggcta catgagccgc taattgaggc
    26221 attctggaag attttgccga accattaacc cgccaaaaag aacctgaacg ccgggttatt
    26281 ggtttcgtcg tggcaatcgt agcccagctc attcagccgg gtttcgaaat ccggttcatg
    26341 gtcgccaagt tcgaacgccg ccagtacgcg cccgtagtcg gtgccatggc tgcgatagtg
    26401 gaacaaagaa atgttccagt gcgtacccag cgtgttgagg aagcgcagca gcgcgcccgg
    26461 tgattccggg aattcgaagc tgtagaggcg ttcctgcaac ggatgtgacg gacgcccgcc
    26521 gaccatatag cgcacatgca acttcgccat ttcgtcgtcg gagagatcca ccacgctgta
    26581 gccgccgtcg ttgagcatct gcaaaatttc tttgcgctct tcgaggccgc ggctcaggcg
    26641 cacgccgaca aagatgcagg cgtttttggc atcggcaaaa cggtagttga actcggtgac
    26701 cgatcgcccg ccaagcagtt ggcagaattt gaggaagctg cctttttctt ccggaatggt
    26761 caccgccaac aacgcttcac gctgttcgcc cagttcgcag cgttccgaga cgtagcgcag
    26821 gccgtggaag ttcacgttgg caccggaaag aatatgcgcc agccgttcgc cgcgaatgtt
    26881 gtgctgggcg atatattttt tcattcccgc cagcgccagc gcgccagagg gttccgccac
    26941 cgcacgcaca tcttcgaata aatccttcat cgccgcacag atcgcatcgc tatcgacggt
    27001 gatgatgtcg tcgagatact cctggcataa acggaaggtt tcgtcaccga tgcgttttac
    27061 cgcgacacct tcggcgaaca gtcccacgcg tggcagatca accggatgac ccgcatccag
    27121 cgctgctttc aggcaggcgg agtcttccgc ttctacggcg atcactttga tttgcggcat
    27181 cagttgtttg atcagcaccg ctacgcccgc cgccagaccg ccgccgccga ctggcacaaa
    27241 tacgcggtcg agatgggcgt cctgctggag cagttccagc gccagcgtgc cttgcccggc
    27301 aatcaccatc ggatggtcga acggcggcac ccaggtgaac ccctgctgct gtgacagttc
    27361 gatcgcttta gctttcgctt cgtcaaagtt cgcgccatga agcagcactt cgccgccgaa
    27421 tccacgtacc gcatcgactt tgatgtcggc ggtggcggtt ggcataacga tcagggcctt
    27481 cacgcctaac cgcgcagaag aaaacgcgac gccctgcgcg tggttacccg cagaagcagt
    27541 gatcacgccg tgcgcttttt gttcttccgt caggcccgcc atcatggcgt atgcgccgcg
    27601 cagcttaaag ctgtgcactg gctggcgatc ttcgcgcttc accagaatga cgttatcaag
    27661 acgcgacgac agtttttcca ttttttgtag cggcgtaacc tgcgccgcct cgtaaaccgg
    27721 cgcgcgcagc actgctctta aatattcggc accttccgga gcaccggaca ggggttgcga
    27781 gtcagccatt attaaccccc cagtttcgat ttatcgcgca ccgcaccttt gtcggcgctg
    27841 gttgccaggc tggcgtaggc gcacagggca aaggaaacct gacgttcacg gtttttcggc
    27901 gtccaggctt tgtcaccccg agcttcctgc gcttcacgac gcgccgccag ttcggcatcg
    27961 cttacctgta actgaatgcc acggttcggg atgtcgatag cgatcaggtc accatcttca
    28021 atcagaccaa tgctgccgcc gcttgccgct tccggtgaga cgtggccgat ggaaagacca
    28081 gaggtgccgc cagagaaacg accgtcggtg atcagcgcac aggctttacc gagacccatt
    28141 gatttcagga agctggttgg gtagagcatt tcctgcatgc cagggccgcc tttcgggcct
    28201 tcatagcgga tcaccaccac atcgcccgcg acaactttgc caccgaggat cgcttctacc
    28261 gcgtcgtcct ggctttcgta cactttcgcc gggccggtga atttgaggat gctgtcatcg
    28321 acgcccgcgg ttttcacgat gcagccgttt tcagcaaaat taccgtagag caccgccagg
    28381 ccgccgtctt tgctgtaggc gtgttccagc gagcggatac agccgtttgc gcgatcgtca
    28441 tcgagagaat cccaacggca atcctgcgag aatgcctgtg tagtacgaat gcccgcaggg
    28501 cctgcgcgga acatattttt taccgcgtca tcctgggtca gcataacgtc gtattgttcc
    28561 agagtttgcg gcaacgtcag gccaagtacg tttttcacat cacggttcag taaccccgcg
    28621 cgatccagtt caccgagaat accgataaca ccaccagcac ggtgaacatc ttccatatgg
    28681 tatttttggg tgctcggcgc aactttgcat aactgcggga ctttacgcga aagtttgtcg
    28741 atatcactca tggtgaagtc gatttccgct tcctgcgccg ccgccagcag gtgaagtacc
    28801 gtgttggtag agccgcccat cgcgatatcc agcgtcatgg cattttcaaa cgccgctttg
    28861 ctagcaatat tgcgcggcag tgcactttcg tcgttttgct cgtagtaacg tttggtcaat
    28921 tcaacaatgc gtttaccggc attgaggaac aactgcttac ggtccgcgtg ggttgccagc
    28981 agcgagccgt tgcccggctg cgacaggccc agcgcttcgg tcaggcagtt cattgagtta
    29041 gcggtaaaca tcccggagca ggagccgcag gtcggacacg cggaacgttc aacctgatcg
    29101 ctctgggagt cagaaacttt cgggtctgcg ccctggatca tcgcatcaac cagatcgagt
    29161 ttgattatct gatcggacag tttggttttc ccggcctcca tcgggccgcc ggaaacaaag
    29221 atcaccggaa tattcaggcg cagggaagcc atcagcatcc ccggggtgat tttgtcgcag
    29281 ttagagatgc agaccatggc gtcggcgcag tgggcgttga ccatatactc aacggaatca
    29341 gcgatcagtt cgcgagatgg aagtgaataa agcatccccc cgtggcccat ggcaatccca
    29401 tcatccaccg caatggtgtt gaactctttg gcaacgccgc cagccgcttc aatttgttcg
    29461 gcgaccagtt taccgagatc gcgcagatgg acgtgacccg gtacaaattg ggtgaacgag
    29521 ttcacaaccg cgataatcgg cttgccaaaa tcggcgtcgg tcattccggt ggcgcgccac
    29581 agcgcacgag cacccgccat attacgacca tgagtggtgg tggcggaacg gtacttaggc
    29641 atactttatt tactcccagt gtctgtctcg taaatgggac ggtgcgtgcc gtcccatttt
    29701 ttgtatttat tgattaactt gatctaacca gccccattta tcttcggttt cgccagtgaa
    29761 gaggccgaag aaggcttgct gaatgcgttt ggtaaccggg ccacaacggc cttcgccaat
    29821 ctgaataccg tctacgctgc gcactggcgt gatttctgcc gccgtaccgg acataaagac
    29881 ttcatccgcc aagtacaggg attcgcgcga cagcacctgc tcacgcactt caattcccag
    29941 ctctttcgcc agtttgatga tggcatcacg ggtaataccc ggcagcgcgg aggaggtgaa
    30001 cggtggggtg aacagcacac catctttcac ttcaaacagg ttttcgcctg cgccttcaga
    30061 gatataaccg ttcacatcca gcgcgatacc ttcctgataa ccgtggcggc gcgcttcgct
    30121 acccaccagc agggaagaga gatagttacc accggctttt gccgcagttg ggatggtgtt
    30181 tggtgctgcg cggttccagg aggaaaccat cgcatcgatc ccctgctcca gcgcttctgc
    30241 gcccagatac gctccccacg ggaaagcggc gataatcaca tcggttgagt atcccgctgg
    30301 cgggtttact cccatgccaa catcaccgac gaagatcagc ggacggatat aggcgctggt
    30361 gagattgttt ttgcggatca cgtcacgaca agcttccatc agctcatcaa tgctctgtga
    30421 aaccgggaag cgatagattt tggcggagtc atgcagacgc tgcatatgct cacgatggcg
    30481 gaatacaacc ggtcctttgt gtgagtcgta gcaacggatg ccttcaaaaa ccgaggtgcc
    30541 atagtgcagc gcgtgcgaca tcacatgcac cttcgcgtct tcccagcgaa ccatctcccc
    30601 attgaaccaa atgtaatcag ctttcttcgt ggtcattttt atattccttt tgcgctcagg
    30661 cgcggatttg ttgtgatgtg gttgtgctct ggcagatggc aacgtgtgcg acgtccacca
    30721 gtttatttaa ctgactaaac agtaagtcga ccgaccgtgg gctggcaacg gtcaattcga
    30781 tatttatatt ttgtgcatcg ctggcggcag ccatattcat tgagcagacg tggaaaccac
    30841 gatgacgcac cacgcgtaaa acacgttcta aggtttccgg attgaagcga gccgatacat
    30901 tgacctgatg ttgcatcatg ataatttctc caacatttct gaattactgg cacctggcgg
    30961 caccagcggc cagacgttct caagttcgtc gattgagaca tgaagcaggt atggcccatc
    31021 actgttcagc atggtgtcga gtgccgcttc aacctggtct ttacgggtga tgtgttggcc
    31081 agggatgccg aaggcgctgg ctaacatgag gaaatcgggg ttatcggtaa gggtggtttc
    31141 gctgtatcgt tcctgaaaaa acagttgctg ccattgtcga accatcccta accgttggtt
    31201 atcgagtaag acgattttca acggtaactg cttgcgtttt acggtgccca gctcttgcac
    31261 attcatcatg aaagagccgt caccggagat acagacaacg gtatcgttcg gtcgcgcgac
    31321 ttgtgcgcca actgccgccg gtaaaccaaa acccatggta cctaagccgc tggaggtgat
    31381 gaaattttcc gggcgagtgt gggcgatgtg ctgcgcagcc cacatctggt gctgccccac
    31441 atctgtggtc acgacgcaat ccgcaggttt acgatccgac agttgtttta acaacaacgg
    31501 agcgtagata gcgtcaccgg gatggtcgta acgccaggaa tgttcatcac gcagctgcgc
    31561 gcagtgttgc tgccagtcat tgatatttaa cggctgctgt aatgctggta acagagcatt
    31621 taaatcacct tgtaatgcca catgtgcctg acgcagcttg ttcatttctg ccgggtcgat
    31681 atccatatgg ataacactgg cgtgtggtgc gaaggtgttc agtttgccgg tcacccggtc
    31741 atcaaaacgt gcacccacgg cgatcagcag gtcacactcc tgcaccgcga agtttgccgc
    31801 tttggttccg tgcatcccca gcatgcccag atagtacgga taatctgctt ctactgcgcc
    31861 cagccctttc agcgtacagg tggcaggcat ttttgtggca gcgagaaatt cacgcaaagc
    31921 cggaactgcc tgcgccatcc ccacgccacc gccaacgtac agcatcggtt tttgcgcttt
    31981 tgccagcatc tggcgcgctt gctcgacttc ggcatgtggg aaagtcactt cgttttcaac
    32041 ggtggtgaac cacggttcca gatcgccgct ggctaactgg atatcttttg ggatatcgac
    32101 cagaaccgga ccaggacgac ctgagctggc aacgtcgaat gcttcagcca tgatgcgcgg
    32161 caactcttcc agagactgca ccagaaagct gtgcttggta caggctaacg acaatcccag
    32221 gacatccact tcctgaaatg cgtcggtgcc gataaacggt gcggacacct gaccagtgat
    32281 ggcaacgaca gggatggaat ctaacagtgc gtccgcaagc ccggttatca ggttggttgc
    32341 gcccggacca gacgtggcga tacatacgcc agttttaccg gtagcacggg cataaccgat
    32401 agccgccatt gccgcaccct gctcatgtcg gcacagcaag tgctccacgc cgccgtcata
    32461 caatgcatcg taaaccggca taattgcgcc acccggataa ccgaaaacgg tgttcacacc
    32521 ctgtgcccgc aacgcatgta ccacccactg tgcgccattc atagttagtt ccccgtcctg
    32581 aatcttgaga aacagaattt tgtgctgtta ttcattgtct gctcctcggt tatgttttta
    32641 aggtcaaaaa aacccccgga cctttcggtg cgggggtctt agttcgttaa ggcttgatct
    32701 ctaagccttt cctcgtccaa gtgcagcccc gcacggtggg ataataatca ccaccacgct
    32761 aatcacgacc aggctaatca ctcgtagaag ggctgtcatt ttgtcttttc ttgcatcttg
    32821 ttcgaaggaa tgcctaaaga gttaccatag attttgcgaa tagcacaaga tatttttggg
    32881 cctattttta tgatggcttt aataatttca atagaaattg ttgtttttta aaaagaaaat
    32941 agtgagtgaa aaaattcaat ttcagtataa atgttgttcc cttcctcgta gttcatcctt
    33001 tgtccgctgc atttttgcga gcatcattcc accgccagaa caactcaact tcacttgcac
    33061 aaataaacag gaatcagcca ctaaaattca cgaattagcg ggctacagca gcgatatctt
    33121 atgcacataa tggtgactca aggagggttt atgtcactgt caattgttca tacccgcgca
    33181 gccctggaag taaatgcgcc cccaatcact gttgaggtac atatcagtaa aggtctaccc
    33241 ggcttaacga tggtgggctt aacagaaaca acggtgaaag aagctcgcga tcgcgtgcgc
    33301 agcgccatta tcaatagcgg atatgaatat ccggcgaaaa aaatcaccat caaccttgct
    33361 ccagccgatc tgccaaaaga agggggacga tatgatttac ctatcgccat tgcgttgctg
    33421 gcggcctcag aacagcttac agccaataag ttagatgaat atgaattagt cggagaacta
    33481 gcgcttacag gcgctctgcg tggcgttccc ggtgcaatct ccagtgcaac tgaagctatt
    33541 aagtcgggca gaaaaattat cgtcgcgaaa gataacgaag atgaagtggg gctaattaac
    33601 ggtgaaggat gcctgatagc cgatcatctg caggctgtct gtgcgtttct ggaaggtaag
    33661 aacactctcg aacgcccgaa accaactgat gcagtatccc gggcgctaca acatgatctc
    33721 agtgatgttg tcggtcagga gcaaggaaaa cgaggactgg aaattaccgc cgctggtggg
    33781 cacaaccttt tactgattgg gccgccggga acaggtaaaa caatgctcgc cagccgtatt
    33841 aatggcctgt tgccagattt aagcaatgaa gaggcactgg agagcgctgc gatattaagt
    33901 ctggtaaatg ctgaatcagt acaaaaacaa tggcggcagc gcccgttccg ctcacctcat
    33961 cacagtgcgt cgttaactgc gatggtgggc ggtggtgcaa ttccagggcc aggtgaaatt
    34021 tcgttggcgc ataacggcgt gctttttctt gatgagctac ctgaatttga acggcgtaca
    34081 ctggatgcct tgcgagagcc gattgaatcc gggcagatcc atctttcacg cacacgagca
    34141 aaaataacct atccagcccg tttccagctt gttgcggcga tgaatcccag ccctaccgga
    34201 cattatcagg gaaaccataa ccgctgcacg ccagaacaga cattacgtta tctcactgga
    34261 gatcccatta ccaccccccg gcattttgag taaaacggta gtgccgggag aaagcagcgc
    34321 caccgttaaa caacgcgtaa tggccgccag agagcgccaa tttaagcggc agaataaact
    34381 gaatgcctgg ctggatagtc cggaaatacg ccaattctgc aaacttgaga gcgaagatgc
    34441 gatgtggttg gaagaaacgc tgatccatct ggggttatcg attcgtgcct ggcagcggtt
    34501 actgaaagtt gcacgaacca ttgctgatat tgatcagtct gacattatca cacgtcagca
    34561 tttgcaggag gcagttagct atcgtgcgat tgaccgtttg ctcatccatc tgcaaaaact
    34621 actgacataa aaaaagggca tttcgccctt tttattaatc gtcagaatcg gtgtagtctt
    34681 cagcaccttc aacctgcggt ttaccgccgg aaagggtgtg aaaacgtttt ggacgcttaa
    34741 tacgcgtcat atacttggac cacacgcgtt ctgcttctgt cactggctca cgttcgccac
    34801 ggcatactgc tacgaagagt ttctcttcct cggtaaccgg ctcgcgtttg ccaagatcca
    34861 actcattgaa ggcataacca tgacgctcaa gcagttgtgc ctctttgatg gtgaaatcac
    34921 catgacgaga gaatccacgt ggataatgtt tattgtcgaa atatcgatta gtcgtcgtaa
    34981 agctttccgc catcctgcac gctcctaatt ctttgaccga gctagttatg gcgcggagta
    35041 ttagttacgc ttgacagagt gtaaaacaaa acatttaaat cataacgaca aataattttg
    35101 cggagagcac tgtggatacg gaattgttaa aaactttcct ggaagttagc cgaacgcgtc
    35161 actttggtcg agcggctgaa tcgctctatc tgacccagtc agcagtgagc tttcgaatca
    35221 gacaactgga aaatcaactg ggtgtgaacc ttttcacccg ccacagaaac aatatccgtt
    35281 taaccgctgc cggtgaaaaa ctactgcctt atgcagaaac gctcatgagc acctggcagg
    35341 ccgcccgcaa ggaggtggcg catacctcac gacataacga gttttctatc ggtgccagcg
    35401 cctcgttgtg ggaatgtatg cttaatcagt ggttgggacg cttgtatcaa aatcaggatg
    35461 cccatacagg cttacagttc gaagcgcgaa ttgcccaacg gcagtctctg gtaaaacagc
    35521 tgcatgaacg ccagcttgat cttcttatta ccactgaagc gcccaaaatg gacgaattta
    35581 gtagtcagtt gctggggtat ttcactttag cgctttatac cagtgcccct tctaaactaa
    35641 agggagatct taactatctg cgactggagt gggggccaga ttttcaacag catgaggcag
    35701 gtttgatcgg tgctgacgaa gttcccattc tgacaaccag ttctgctgaa ctggcacagc
    35761 aacagattgc gatgcttaat ggttgcacct ggctacccgt cagctgggcg cgtaaaaaag
    35821 gcggcctgca taccgttgtc gatagcacaa cactttcacg gccgctttat gccatatggc
    35881 tgcaaaatag cgataaaaat gcgttgattc gcgatctttt gaaaattaac gtgctggatg
    35941 atgtgtatta atctgaatgg ctggcaagga tgccggtaga aggatttact tcggagagga
    36001 ttatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctggc aggggcggag
    36061 agactcgaac tcccaacacc cggttttgga gaccggtgct ctaccaattg aactacgccc
    36121 ctaattaggg tggcggaacg gacgggactc gaacccgcga ccccctgcgt gacaggcagg
    36181 tattctaacc gactgaacta ccgctccacc gaattctttt acaaccaccg gttttacgac
    36241 cggctt
//
LOCUS       DAJTQQ010000033        35535 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000033 DAJTQQ010000000
VERSION     DAJTQQ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.033"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(68..826)
                     /gene="deoR"
                     /locus_tag="OO309_001808"
     CDS             complement(68..826)
                     /gene="deoR"
                     /locus_tag="OO309_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR5846968.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(884..1480)
                     /gene="ybjG"
                     /locus_tag="OO309_001809"
     CDS             complement(884..1480)
                     /gene="ybjG"
                     /locus_tag="OO309_001809"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5846969.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1765..2991
                     /gene="mdfA"
                     /locus_tag="OO309_001810"
     CDS             1765..2991
                     /gene="mdfA"
                     /locus_tag="OO309_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCR5846970.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3032..3316)
                     /locus_tag="OO309_001811"
     CDS             complement(3032..3316)
                     /locus_tag="OO309_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5846971.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3402..4217)
                     /gene="ybjI"
                     /locus_tag="OO309_001812"
     CDS             complement(3402..4217)
                     /gene="ybjI"
                     /locus_tag="OO309_001812"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR5846972.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4217..5425)
                     /locus_tag="OO309_001813"
     CDS             complement(4217..5425)
                     /locus_tag="OO309_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5846973.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5509..6045
                     /gene="rcdA"
                     /locus_tag="OO309_001814"
     CDS             5509..6045
                     /gene="rcdA"
                     /locus_tag="OO309_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR5846974.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6220..7905)
                     /locus_tag="OO309_001815"
     CDS             complement(6220..7905)
                     /locus_tag="OO309_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR5846975.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8175..8552
                     /locus_tag="OO309_001816"
     CDS             8175..8552
                     /locus_tag="OO309_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR5846976.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8582..8839)
                     /gene="grxA"
                     /locus_tag="OO309_001817"
     CDS             complement(8582..8839)
                     /gene="grxA"
                     /locus_tag="OO309_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR5846977.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            8999..9286
                     /locus_tag="OO309_001818"
     CDS             8999..9286
                     /locus_tag="OO309_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR5846978.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9270..9992
                     /gene="nfsA"
                     /locus_tag="OO309_001819"
     CDS             9270..9992
                     /gene="nfsA"
                     /locus_tag="OO309_001819"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR5846979.1"
                     /translation="MTPTIELICGHRAIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10053..10955
                     /gene="rimK"
                     /locus_tag="OO309_001820"
     CDS             10053..10955
                     /gene="rimK"
                     /locus_tag="OO309_001820"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR5846980.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11043..11519
                     /locus_tag="OO309_001821"
     CDS             11043..11519
                     /locus_tag="OO309_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR5846981.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            11871..12983
                     /gene="potF"
                     /locus_tag="OO309_001822"
     CDS             11871..12983
                     /gene="potF"
                     /locus_tag="OO309_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR5846982.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13078..14211
                     /gene="potG"
                     /locus_tag="OO309_001823"
     CDS             13078..14211
                     /gene="potG"
                     /locus_tag="OO309_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR5846983.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14221..15174
                     /gene="potH"
                     /locus_tag="OO309_001824"
     CDS             14221..15174
                     /gene="potH"
                     /locus_tag="OO309_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR5846984.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15171..16016
                     /gene="potI"
                     /locus_tag="OO309_001825"
     CDS             15171..16016
                     /gene="potI"
                     /locus_tag="OO309_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR5846985.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16076..16564
                     /locus_tag="OO309_001826"
     CDS             16076..16564
                     /locus_tag="OO309_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR5846986.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16605..17732
                     /gene="rlmC"
                     /locus_tag="OO309_001827"
     CDS             16605..17732
                     /gene="rlmC"
                     /locus_tag="OO309_001827"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR5846987.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(17907..18638)
                     /gene="artJ"
                     /locus_tag="OO309_001828"
     CDS             complement(17907..18638)
                     /gene="artJ"
                     /locus_tag="OO309_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR5846988.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(18930..19598)
                     /gene="artM"
                     /locus_tag="OO309_001829"
     CDS             complement(18930..19598)
                     /gene="artM"
                     /locus_tag="OO309_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR5846989.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19598..20314)
                     /gene="artQ"
                     /locus_tag="OO309_001830"
     CDS             complement(19598..20314)
                     /gene="artQ"
                     /locus_tag="OO309_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR5846990.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20321..21052)
                     /gene="artJ"
                     /locus_tag="OO309_001831"
     CDS             complement(20321..21052)
                     /gene="artJ"
                     /locus_tag="OO309_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5846991.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEIITVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21070..21798)
                     /gene="artP"
                     /locus_tag="OO309_001832"
     CDS             complement(21070..21798)
                     /gene="artP"
                     /locus_tag="OO309_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR5846992.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22016..22531)
                     /locus_tag="OO309_001833"
     CDS             complement(22016..22531)
                     /locus_tag="OO309_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5846993.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23180..24949
                     /locus_tag="OO309_001834"
     CDS             23180..24949
                     /locus_tag="OO309_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5846994.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25160..25483
                     /locus_tag="OO309_001835"
     CDS             25160..25483
                     /locus_tag="OO309_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR5846995.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25480..26310
                     /gene="amiD"
                     /locus_tag="OO309_001836"
     CDS             25480..26310
                     /gene="amiD"
                     /locus_tag="OO309_001836"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR5846996.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26307..27320)
                     /locus_tag="OO309_001837"
     CDS             complement(26307..27320)
                     /locus_tag="OO309_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5846997.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27419..28849)
                     /locus_tag="OO309_001838"
     CDS             complement(27419..28849)
                     /locus_tag="OO309_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR5846998.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(28860..29861)
                     /gene="ltaE"
                     /locus_tag="OO309_001839"
     CDS             complement(28860..29861)
                     /gene="ltaE"
                     /locus_tag="OO309_001839"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR5846999.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(29898..31616)
                     /gene="poxB"
                     /locus_tag="OO309_001840"
     CDS             complement(29898..31616)
                     /gene="poxB"
                     /locus_tag="OO309_001840"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR5847000.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31749..32717)
                     /gene="hcr"
                     /locus_tag="OO309_001841"
     CDS             complement(31749..32717)
                     /gene="hcr"
                     /locus_tag="OO309_001841"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR5847001.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32729..34381)
                     /gene="hcp"
                     /locus_tag="OO309_001842"
                     /gene_synonym="priS"
     CDS             complement(32729..34381)
                     /gene="hcp"
                     /locus_tag="OO309_001842"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR5847002.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34525..35424)
                     /gene="lysO"
                     /locus_tag="OO309_001843"
     CDS             complement(34525..35424)
                     /gene="lysO"
                     /locus_tag="OO309_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR5847003.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         8854 a         8793 c         9123 g         8765 t
ORIGIN      
        1 ttcggcagct ggttctctta atcttctgat aaccggatgg cgcgaaacgt catccggtta
       61 tacgtcatta atacatcaac ttaatacggt gcgtctgcgc gtacttcaca tactcatctt
      121 ccgggcaaca atcactcacc acaatatcaa agcgtttcag gtcacccatg cgcgccggac
      181 gcaccttgcc aaatttactg tggtcgacaa ccagcacatg cttttgcgcc atcgacatgg
      241 cccagtgttt taccggcaac tcttcaagat taaaacaggt agcgccttta ctgacatgca
      301 cgcccgccgc ggaataaaaa gcgatatccg ggcaaaaatt attcaacgtt tgctgaaaat
      361 cgatgggttt gaaaatggcg ttgctggcgt gaaattcacc accgcacaga aatgcgcggc
      421 aatggggttt ctctttcagc gccagaaagg tatttagcga ataacaaacg gcggtaaaag
      481 ggatttcatt atcaatcgct tcaataatcc acggcgtggt ggtgccacag tcaaaaaaga
      541 gggtctgatc gggttctacc agtgtcgcag ccagttttgc cgcccggcgt ttttcttcca
      601 ccaggcggga tttttgatcg cttaacaggt aatggctggc actgcgcggt tccagaacaa
      661 tatagccgcc gagcaaaacg acgggcgcac tgtggttgtt cagatcgcga cgaatcgtca
      721 tctccgaaac cccaagcagg gcggcggcgt ctttaagatg taacttatcg ctgcgtttta
      781 attcttgcag cagctgcccg atacgctctt cgcgacgtgt ttccataatc cctctgaata
      841 gttattgaag cgagccgctc aatactacac tttttagcag agatcagtca cgcacccagc
      901 ctttgcggat cggtaatgca aaacagacgc gataccacga ttgcagacgt tgatagagtt
      961 tatgccccat cgcttgccag ataatctggg cactaaggca gccaatcata cctgccagca
     1021 atccaccgag catatccagc ggccagtgga cgccaagata aacgcgcgac caggcaatga
     1081 cgacggccag caccattaaa agtgagccgg accacaggcg atgccagcat aaaaatgcca
     1141 gtgcaaaggt gaaaatcacc gtaccgtgat cgctcgggaa tgagtcatcc gccgcatgat
     1201 gcaggaagtt atagccgata ttttcgacaa agggtcggtc gtgcggaaaa agatgtccca
     1261 tcgtccagga cacaaacagg ctgaccgcca gcgcgatagc gatttttatc accagttgcc
     1321 gttgtgctgt aagcccccac aaccaaagta ccgcggccag caacggcacc acggtaatca
     1381 aatctttagc aataaaaatc gccaacgaga tcatccacgg agccgagtct ggcgtcgcgt
     1441 taataagaga gaatagagag agattcaaat tttccagcat aacttcccga cgcaaagtga
     1501 ttaaaaggga gccaatacag gcaagtcgtt gagaataaag tgcaggttaa actgggtaaa
     1561 gcggcatcgt cttatttccc tcaagcggcc tgtttacggt gggtgattgt aacgggcata
     1621 ggttaaataa aacttaaaga aagcgtagct atactcgcaa taatgtaaga atgtgcttaa
     1681 ccgtggtttc agctacaaaa ttcgctttct cgttagctgc gcttttatta aactctgcgc
     1741 gattattatt ggcgaagaaa ttgcatgcaa aataaattag cttccggtgc caggcttgga
     1801 cgtcaggcgt tacttttccc tctctgtctg gtgctttacg aattttcaac ctatatcggc
     1861 aacgatatga tccaacccgg tatgttggcc gtggtggaac aatatcaggc gggcattgat
     1921 tgggttccta cttcgatgac cgcgtatctg gcgggcggga tgtttttaca atggctcctg
     1981 gggccgctgt cggatcgtat tggtcgccgt ccggtgatgc tggcgggtgt ggtgtggttt
     2041 atcgtcacct gtctggcaat attactggcg caaaacattg aacaattcac cctgttgcgc
     2101 ttcttgcagg gcataagctt ctgtttcatt ggcgctgtgg gatacgccgc aattcaggaa
     2161 tccttcgaag aggcggtttg tatcaagatc accgcgctga tggcgaacgt ggcgctgatt
     2221 gctccgctac ttggtccgct ggtgggcgcg gcgtggatcc atgtgctgcc ttgggagggg
     2281 atgtttgttt tgtttgccgc attggcagcg atctcctttt tcggtctgca acgagctatg
     2341 cctgaaaccg ccatgcgtat aggcgagaaa ctgtcactga aagaactcgg tcgtgactat
     2401 aagctggtgc tgaagaacgg ccgctttgtg gcgggggcgc tgggattcgt tagcctgcca
     2461 ttgctggcgt ggatcgccca gtcgccgatt atcatcatta ccggggagca gttgagcagc
     2521 tatgaatatg gcttgctgca agtgcctatt ttcggggcgt taattgcggg taacttgctg
     2581 ttagcgcgtc tgacctcgcg ccgcaccgta cgttcgctga ttattatggg cggctggccg
     2641 attatgattg gtctgttggt cgctgctgcg gcaacggtta tctcatcgca cgcgtattta
     2701 tggatgaccg ccgggttaag tatttatgct ttcggtattg gtctggcgaa tgcgggactg
     2761 gtgcgattaa ccctgtttgc cagcgatatg agtaaaggta cggtttctgc ggcgatggga
     2821 atgctgcaaa tgctgatctt taccgtcggt attgaaatca gcaaacatgc ctggctgaac
     2881 gggggcaacg gactgtttaa tctcttcaac cttgtcaacg gaattttgtg gctgtcgctg
     2941 atggttatct ttttaaaaga taaacagatg ggaaattctc acgaagggta aaaaaatgcc
     3001 tgattgattt gtgcgatcag gcattctcga attaatggtg atggtcgtca atctggtgtt
     3061 cgataaccat cccttcacct acgctggcaa gatggcgaac ataaggatgc gggcggtaag
     3121 ccggagctgg cgcaggagcc acatagacgg tttgcggcac agtcgcgacg ctgaccgctt
     3181 gtggaacgct gactgaatca ggaaccacaa ccactttgta tccactcggc acatcgacgg
     3241 tgacactttg cgccatcgcc acaccagtaa agcccattaa aagcgcgccc aacagtagac
     3301 aatttttcat aatcatttcg cctgaaataa tgcagcgggt taatttccgc gaattatgca
     3361 gccttcattc aggcattgtg ttgccgggcg tgctgccgtt tttattggtc aaatggcgct
     3421 tcgtgtttaa gaactttatc gatcacatcc agtacgcctt cacggttatt gtagcctgcc
     3481 cggtattttg ccgctgcgac gactgcgctg ccggcatttt ccattgcaaa actaaagcct
     3541 gcctgacgca gcatctcaat atcgttaccg ccatcgccaa agaccaccac ttcgctgtcg
     3601 tctattcccc ataatttctg cagttggcga aggccattgg ctttatgtac gccggggata
     3661 atcagatcga tgctgccgtt gccggtgtgg actggcacca taatatcgcc gatggcctca
     3721 tgtaatgctt tttgtacttg tggaatcagt tcatcggaaa gattcaggcc aaacttaaag
     3781 aaaatatctt ctaagttgtc aaagttatcg acgtattcca gacggtgata atacatttcc
     3841 gccaccgttt tcatggcatc gtcatatttt ttgagtgtat aggcgctatt ttttccgcag
     3901 gcaataattt ccacttccgg gcgcgtcagc aaatgttcca cgacagtagc aaacgcatcc
     3961 ttcgacagct cgccattaaa aacatctttg ccttcactca ttacccagcc gccgttttca
     4021 gccacaaagg caatttcatt cgcaatttca gggaagaaag agatcaactg ataatattga
     4081 ttcccgctgg cgaccacaaa gcgaatgcct tgtgctttca tttgctgata ctgagccata
     4141 aaccgctcac ggttataggt tttttgatcg cttaagaaag taccatccat gtctaccgca
     4201 attaatttaa tgctcatcaa ctattctcca tcgccgtctg cgttttggta tcgggtttgg
     4261 cgacggcttt cgcgacaata gccgcgagaa taaccagcgc cagtacaacc agcattgcac
     4321 tacgtaatcc ataatgttcg ccgagatagc ccagcagcgg cggcccgacg aggaaagcca
     4381 gataaccggt cgttgctacc acactgacgc gggttggtgc atcggggccg gtatcgctgg
     4441 cggcagaaat ggtcagcggg aagcccagcg aggcacccag tccccaaagt acaacagaca
     4501 ctcctgcgac ccaggcgcta tcgacaaaaa taatcagccc aatacccaac gcccccatta
     4561 gtgcactggc ccgaaccacg gcaacgcgac tgtaactgtc gatgaaccaa ccgccggtga
     4621 agcgtccaac ggtcatcccc agggtaaaac cggcataaat cagtgagccg gaagtggggc
     4681 taaaaccgtg accatcaacc attaataagg gtaaccagtc gttggcagaa ccttcggcaa
     4741 aggccatcgc cagcactaca acaccaatca acagcaactg gatatcgcga taaaaaggaa
     4801 tgcctttttc gccatgctgg gtgccatcgg cagcattttt gcccgtaccg tcagggattg
     4861 cctgaatggc gatataaata ggcgcgatac ctaccagcgc cgccaataaa atgtgcaccg
     4921 ttgccggaac gccaaaggcc gtcagtgcca tcccgacacc agcgcctgcc agcgtgccca
     4981 ggctataaaa accgtgcatc atcggcaaaa ccgttttatt catttctcgc tcaacggcgg
     5041 caccttcaac gtttatcgcc acttccgcag aaccaaaact tgccccaaag acgccgagac
     5101 caacggcaaa gagcaggggc gatgtcagcc agagtgccag acttaatatc atcatcccga
     5161 tcaatgcgca ggacatcgtg accaggatga cattacgtgt cccaaagcgt ttcactaacc
     5221 acgccgagca gagaataccg ctcatcgaac cgatcgacag accaaagaga acaccgccca
     5281 tttcagcgat cgagacagag agaatatcgc ggatagcagg cgtacgggtt gcccaggacg
     5341 ccattaacag gcctggcaaa aagaagaaca taaacagcgc ccaggttcgg cgtttcaatg
     5401 cattacgtga agaatttacg gtcatagatc acgtcaaaat aagaagaggg aagacaacat
     5461 tagcaaggtt gtgtacattt gtacacaatt gcagacagag gaaatgacat gcgtcgcgct
     5521 aacgatccgc aacggcgaga aaaaattatc caggccacgc tggaggcggt gaaactttac
     5581 ggaatacatg ctgttacgca ccgcaaaatt gctacccttg ccggggtacc gttgggatcg
     5641 atgacctact atttttcagg aattgatgag ttgttgctgg aggcgttcag cagttttact
     5701 gagattatgt cccggcaata tcaggcattt tttagcgatg ttagtgatgc tcaaggcgca
     5761 tgccaggcta tcaccgatat gatctacagc tcacaggttg caacgccgga taatatggag
     5821 ctgatgtacc agctctacgc gctggctagc cgaaaaccgc tattaaaaac ggtaatgcaa
     5881 aactggatgc agcgcagtca gcaaacgctc gaacaatggt ttgaacccgg aaccgcccgc
     5941 gcgcttgatg cgtttattga ggggatgacg ctgcattttg tcaccgaccg taagccgcta
     6001 tcgcgcgagg agattttgag gatggttgag agggttgcag ggtagtagat aaattttaga
     6061 taacaaaaaa cccatcaacc ttgaaccgaa atggcggggt tgatgggctc cacaaaatgg
     6121 ggacatcaaa gaaaagcagt ggcactaatt aagactgatg ccctgcggaa aagttctgcg
     6181 gttgtgcaaa aaaatttcat tttcagggca acttcagttt tatcctaatc ctggccatac
     6241 catgacgatg attgtccctg ccagcgtcag caggacgttg gcgattgcgt aggtgcccgc
     6301 atagcccagc gcagggatgt tactgcgagc tgtatcactg atgatctcca ttgccggcgc
     6361 gcaggtgcgt gcgcccatca ttgcgccgaa caacagtgcg cggttcattc gcaatacata
     6421 agcaccgaac aagaaacaga taaccacggg cacaagactg acaattaatc cggcaatcaa
     6481 catctgaccg ccaatcgcgc ccaggccgtt attaataccg ctaccggcgc tcagaccaac
     6541 gcctgccata aacaccatca agccgaactc tttcaccatg cttaatgcac cttgcggaat
     6601 gtaaccgaag gtcgggtggt tagcacgcat aaagcccagc ataattccgg cgaataacaa
     6661 cccggcagcg ttccccatgc cgaaactgaa tgtgctgaac tggaaggtga tcatcccgat
     6721 catcagccca ataacaaaga aggcgcaaaa tgccagcagg tcagtgacct ggctgtgaat
     6781 cgagataaag ccgatgcgat cggcgatggt ttttacgcga cgggcatcac cgctgacttg
     6841 taaaacgtca cctttgttaa gcacgacgtt gtcatctatc ggcatctcaa tctggctacg
     6901 aatgacgcgg ttaaggaagc aaccgtgatc ggtcaacttc agttgtgcga gacgtttacc
     6961 tacagcgtta tggtttttaa cgaccacttc ttcagtgacg atacgcatgt cgagaaggtc
     7021 acgatcgaaa acttctttac cgttacggaa gctgggatcg agtcgggcat gggcgtcggg
     7081 atagcctacc aacgctattt catcgcccat ttgtagcacg gcatcaccgt ctggatttgc
     7141 cagaatcccg ttacgtcgaa tacgttcaat gtagcagccg gtttgtcgat aaatacccag
     7201 ttcacgcaga tttttgccgt cggtccaggc caccagctcc gggccgacgc gataggcgcg
     7261 gatcaccggt aaataaacct tacggttggc atcagtgtcc aggccacgtt cgcgggcgat
     7321 ttgctgggcg ctggtctgta agtcctgatg ctgcaatttc ggcaagtaac gcgcaccaac
     7381 aatcaaactc accagaccga ttaaataggt taaggcatac ccgaggctca gattatccag
     7441 tgccagtgag agctgcctgc tttccatgcc ggaatgatgc agtgtatcgc cagcaccgac
     7501 cagaaccggt gtcgacgtca tagagcctgc taacataccg gccgtcaggc caatatccca
     7561 gccaaacagc ttacctaacc ctaaggcgat caccagcgca ctgccaacca tcaccagtgc
     7621 taacattagg taatttttcc catcgcgaaa aaaaatggaa aaaaagttcg gtccggcttc
     7681 gaccccgacg cagaaaataa acagcataaa gccaagatta agcgcatcgg tgttaatgct
     7741 gaaatgttgt tggcctaata acagcgatac gactaaaacg ccaatggaat tacccagttg
     7801 gatcgaacca agtcgtaact ttccgagaca tagcccaagc gcgaggacca caaataataa
     7861 cagaatgtaa ttcccattta acaattcggc gacgtttata ttcacggagg ctaacttctt
     7921 gtttactagt aagctgttga aagaaatgct aatttacgat aatgtgtttt accagaattc
     7981 agggcgcaga ttcattcagc gcacctaaac gatagtaaag taacaatata ttttactagt
     8041 gtaatcacat taggtatcaa cggctatatg aattgcgttg gcctatatta gcatggaatg
     8101 cgaagcggct ttatcttact gaacgccaca ctggcgaaaa atgtgttcga tagacgcagt
     8161 gtcaggagga acgagtgaaa cataaacaac gttgggcggg ggcaatctgc tgttttgtcc
     8221 tcttcattgt ggtgtgcctt tttctggcga cgcacatgaa aggcgctttt cgggctgccg
     8281 ggcatcctga aatcggcttg ctgtttttca ttcttcctgg agcagttgcc agtttctttt
     8341 cacagcgtag agaagtcctg aaacctctat ttggcgcaat gctggcggca ccctgttcaa
     8401 tgctcattat gcggctgttt ttttcaccga cgcgctcatt ctggcaagag ctggcatggt
     8461 tactaagcgc ggtgttctgg tgtgcgctgg gggcactgtg tttcttattt atcagtagtt
     8521 tgtttaaacc acagcacaga aaaaatcagt aaagccctca acgcgagggc ttgtcagacg
     8581 atcaggcgtc cagattttct ttcacccatg cagcaaaatc ggtatagccg ccgatatgtt
     8641 gctgatcgac aaaaatctgc ggcacggttt ctacgggttt acctgccttt tgttgtagat
     8701 cttctttagt gatcccttcc gcacgaatat ctacatactg atactgaaaa tcatcgcgtt
     8761 cattgctcaa tttctcagcc agatcttttg cacgcacaca gtaagggcaa cccgaacgac
     8821 caaaaataac ggtttgcatt atttctctcc tcatagattt atgcctgtaa tgatcacgct
     8881 aaaatgtatt cgctgaaagt aggtttaacc tgttgcatta attgctaaaa gctataactg
     8941 ttaaacacaa tacagtgaaa agttttagac tgaaggctca ctttgcagag ggaagcgtat
     9001 gcgcgcgatc ggtaaattgc ctaaaggcgt gttgatactg gaatttatcg gaatgatgct
     9061 actggcggtg gcgctgctgt cggtaagcga ctccctgtcg ctgcctgagc cattttctcg
     9121 gccagaagtg cagattctga tgatttttct cggtgttttg ctcatgcttc ccgctgcggt
     9181 ggtggttatt cttcaggtgg caaaacgtct tgccccacag ctgatgaact gtccaccgca
     9241 atattcacgt tcagaaagag aaaaagataa tgacgccaac cattgaactt atttgtggcc
     9301 atcgcgccat tcgccatttc actgatgaac ccatttccga agcgcagtgt gaggcgatta
     9361 ttaacagcgc ccgtgcgacg tccagttcca gttttttgca gtgtagtagc attattcgca
     9421 ttaccgacaa agcattacgt gaagaactgg tgacgctgac cggcgggcaa aaacacgtag
     9481 cgcaagcggc ggagttctgg gtgttctgtg ccgactttaa ccgccattta cagatctgtc
     9541 cggatgctca gctcggcctg gcggaacaac tgttgctcgg tgtcgttgat acggcaatga
     9601 tggcgcagaa tgcattaacc gcagcggaat cgctgggatt gggcggggta tatatcggcg
     9661 gcctgcgcaa taatattgaa gcggtgacga aactgctgaa attaccgcag catgttctgc
     9721 cgctgtttgg gctgtgcctt ggctggcctg cggataatcc ggatctcaag ccgcgtttac
     9781 cggcctccat tttggtgcat gaaaacagct atcaaccgct ggataaagac gcactggcgc
     9841 agtatgacga gcaactggcg gaatattacc tcacccgtgg cagcaataat cgctgggata
     9901 cctggagcga tcatatccgc cgaacaatca ttaaagaaag ccgcccattt attctggatt
     9961 atttgcacaa acagggttgg gcgacgcgct aaaaccgcca cgtcgatgta tgatacgcgg
    10021 gcttttgacc aggtctgaca gagaggtgca gggtgaaaat tgccatattg tcccgggatg
    10081 gaacgctcta ttcgtgtaag cggctgcgtg aagccgctat acagcgcggt cacctggttg
    10141 aaattcttga tccgctttct tgctacatga acataaatcc tgcggcgtct tctatttact
    10201 acaaaggccg caagttaccc cattttgacg cagtgatccc gcgtattggc accgccatta
    10261 ccttttatgg gacggcggca ctgcgccagt tcgagatgct ggggagctat ccgctcaatg
    10321 agtcggtcgc cattgcccgg gcgcgtgaca aattgcgttc catgcaactg ctggcgcgtc
    10381 agggcatcga cctgcctgtc acgggcattg cgcattcgcc ggatgatacc agcgatttaa
    10441 tcgacatggt cggtggtgcg ccgctggtgg tcaagttggt tgaaggcacg cagggaattg
    10501 gcgtcgtgct ggcggagact cgtcaggcgg cggaaagcgt gattgacgct ttccgcggtc
    10561 tgaacgcgca tattctggtg caggaatata tcaaagaggc gcaagggtgc gatatccgct
    10621 gtctggttgt tggtgatgaa gtggtcgctg cgattgaacg gcgggcgaaa gagggctatt
    10681 ttcgttccaa tttgcatcgt ggcggcgcgg caagcgtcgc cagtatcaca ccacaggagc
    10741 gtgaaatcgc gataaaagcc gcgcgaacga tggcgctgga cgttgctggt gtggatattc
    10801 tgcgtgctaa tcgcgggccg ttggtgatgg aagtgaatgc gtcgccgggg ctggaaggaa
    10861 tagaaaaaac caccggtatc gatatcgcgg gtaaaatgat ccgctggatc gaacgccacg
    10921 ctacgacaga atattgcctg aaaacgggtg gttagtcgca atcacattac tgatcatggt
    10981 tttgcctgcg ctttttgcgt aagctgtgcc ggtcttttta tcgaaagagg ttgtacaaaa
    11041 ttatgacatc gctggtcgtt cctggtctgg atacgctgcg tcaatggctc gatgacctgg
    11101 ggatgagttt ttttgaatgt gataactgtc aggctctgca tctgccccat atgcagaatt
    11161 tcgacggtgt ctttgatgcc aaaatcgatc tgatcgataa cacgatcctg ttttctgcca
    11221 tggcggaagt ccgaccttca gccgtattgc cgctggcggc ggatttatct gccatcaatg
    11281 ccagttcgct gaccgtgaaa gcatttcttg atatgcagga tgataatctg ccaaagctgg
    11341 tggtttgcca gtctttatcc gttatgcagg gcgtaaccta tgagcagttt gcatggttcg
    11401 tgcgtcagag cgaagagcag atttcgatgg tcattcttga agctaatgcc catcaactgc
    11461 tgttaccgac tgacgatgaa gggcaaaaca acgttaccga aaactatttt ctccactgat
    11521 aattcctttc gagcacgcag tcgctgccgc agtgactgcg cgctgcaaaa ttatctgctg
    11581 tttttaacct tttcttaaag attatttcac ttctcttgtg tcgatttggc tttatcacat
    11641 agagcaaata cgcataaaaa tttgttaaat accgtttttt aatccgagct atagtctcaa
    11701 accctggcta aagttattct tgcgatgctt ctatatagcg agcagtgctg gccgggagag
    11761 agttctcttt tcttacaccg cgccgataaa aaaatatgca cgtttattgc atatctttca
    11821 gtgtgacaac ttttgttcgt ttgttaacga actttcagaa ggaaagagat gtgaccgcct
    11881 taaataaaaa atggctatcg ggtctggttg cgggtgctct gatggccgtc tctgtcggca
    11941 cgctcgcggc tgaacaaaaa acactccaca tttataactg gtctgattat atcgcccctg
    12001 acacggtggc caattttgaa aaagaaaccg gtattaaagt cgtctacgat gttttcgact
    12061 ctaacgaagt actggaaggc aaattaatgg cgggtagcac cggctttgac ttggtggttc
    12121 catctgccag ctttctagaa cgccagttga ctgcgggagt tttccagccg ctggacaaaa
    12181 gcaaattgcc ggagtggaag aatctcgacc cggagctgct gaagctggtc gccaaacacg
    12241 atcccgacaa taaatttgct atgccctata tgtgggcgac gaccgggatt ggctataacg
    12301 ttgataaagt taaagctgtg ctgggcgaaa acgcgcccgt cgatagctgg gacttgatcc
    12361 tcaaacctga aaatctggaa aaactgaaaa gctgcggtgt ctctttcctg gatgcgccag
    12421 aagaagtttt tgctaccgtg ttgaattatc tcggcaaaga tcccaacagc actaaagcgg
    12481 atgattacac cggaccggca accgatctgc tgttaaagct gcgcccgaac attcgttatt
    12541 tccattcatc tcaatacatt aacgacctgg caaacggcga tatttgcgtc gctatcggct
    12601 gggcaggtga tgtctggcag gcgtcaaacc gcgcgaagga agcgaagaat ggagtgaatg
    12661 tctcgttctc gattccaaaa gaaggggcga tggcgttctt tgatgtattc gccatgccag
    12721 cggatgccaa aaacaaagac gaagcctatc agttcctgaa ttacctgctg cgcccggatg
    12781 tagtagcgca tatttccgac catgtgttct atgtcaacgc caataaagca gccacgccgc
    12841 tggtgagtgc ggaagtccgt gagaacccgg gtatttatcc gcctgcggat gttcgtgcga
    12901 agctgttcac tctgaaagtg caggacccga aaatcgaccg tgtgcgcacc cgcgcgtgga
    12961 ccaaagtgaa gagcggaaaa taatccgcag ccttagatgc cgggcgggcg caccacaccc
    13021 gccggcaatt cgcaccatca tggtgcgctt gcacacattc aatgccggag agcagccgtg
    13081 aatgacgcta tccctcgccc gcaggcgaaa acccgtaagg cgctgacgcc gctattagaa
    13141 atccgcaacc tcaccaaatc ctacgatggt caacatgcgg tggatgatgt cagcctgacc
    13201 atctacaaag gtgaaatctt cgcgctgctg ggcgcatccg gctgtggcaa gtccacgctg
    13261 ctgcgtatgc tggcaggttt cgaacaacct tctgccggac agataatgct tgatggcgtc
    13321 gatttgtcac aggttccgcc ttacctgcgc cccatcaata tgatgtttca gtcttacgcg
    13381 ctgtttccgc atatgaccgt ggaacagaac atcgcttttg gcctgaaaca ggacaaacta
    13441 ccgaaagcgg aaatttccag tcgggtcaat gagatgctcg gcctggtgca catgcaggag
    13501 ttcgccaaac gtaaaccgca tcagctttct ggtggtcagc gacagcgtgt ggccctggcc
    13561 cgaagccttg ccaagcgccc gaaactatta ctgctcgacg agccgatggg cgcgctggat
    13621 aaaaagctgc gtgaccggat gcagcttgaa gtggtggata ttctggagcg cgtcggtgtg
    13681 acttgtgtga tggtcaccca cgatcaggaa gaggcgatga ccatggcggg gcgcatcgcc
    13741 attatgaatc gtgggaaatt tgtccagatt ggcgaaccgg aagagatcta cgagcatccg
    13801 actacccgct atagcgccga atttattggc tcggtaaacg tctttgaagg cgtactcaaa
    13861 gagcgtcagg aagatggctt ggtgcttgat tcgccggggc tggtgcatcc actgaaagtc
    13921 gacgcggatg cctcggtggt cgataacgtg ccggtacatg tggcgctgcg cccggaaaaa
    13981 atcatgcttt gcgaagagcc gcccgccaat ggttgtaact tcgcggtggg ggaggtgata
    14041 cacattgcct atctcggcga tctttcggtg tatcacgttc gtctgaaaag tgggcagatg
    14101 attagtgccc agctacaaaa cgcccatcgt catcgtaaag ggttaccgac ctggggcgac
    14161 gaagtgcgtt tgtgctggga agtggacagc tgtgtggtgc tgacggttta aggagcaaag
    14221 atgaatacac ttgaacctgc tgcccagtcg aaaccgccgg gcggatttaa gctgtggttg
    14281 tcgcagctgc aaatgaagca tgggcgcaaa ctggtcattg cgttgccata tatctggttg
    14341 atcttgctgt ttctgctgcc atttctgatt gtctttaaaa taagcctggc ggagatggcg
    14401 cgcgctattc caccttatac cgaactgatg gagtgggctg acgggcaact gtccatcact
    14461 cttaatctcg gtaattttct gcaactgacc gacgatccgc tctatttcga tgcttatctc
    14521 cagtcgctac aggtggctgc gatttcgaca ttttgctgtt tactgatcgg ctatccgctg
    14581 gcgtgggcgg tggcgcacag taagccttcg acccgtaata ttttattact actggtgatc
    14641 ctgccgtcgt ggacctcgtt tctgatccgc gtttatgctt ggatgggaat attaaaaaac
    14701 aacggtgtgc tgaataattt tctgctgtgg ctgggggtta tcgatcaacc gctgaccatt
    14761 cttcatacca atctggcagt ttatattggc attgtttacg cctacgtgcc gtttatggtg
    14821 ctgccgattt ataccgcgtt gattcgtatt gattattcgc tggtggaagc agcgctggat
    14881 ctcggtgcac gaccgctgaa aacgttcttt actgtgatcg tgccgctgac taaaggtggg
    14941 attattgccg gatcgatgct ggtgtttatc ccggctgtgg gcgagtttgt gatcccggaa
    15001 ctgctcggtg gcccggacag catcatgatc gggcgcgtgc tatggcaaga gtttttcaat
    15061 aaccgcgact ggccggtggc ctcggcggta gcgatcatca tgttgctgct gctaattgtg
    15121 ccgataatgt ggttccacaa acaccagcaa aaaagcgtgg gagaacacgg atgaataatt
    15181 taccggtagt tcgttcgccc tggcggattg tgattttgct gctgggcttt acctttctct
    15241 acgcgccaat gctgatgctg gttatctatt cgtttaacag ctcgaagctg gtgacggtgt
    15301 ggggcggctg gtcaacgcgc tggtatggtg agttattgcg cgatgacgcg atgatgagtg
    15361 cggttggttc aagcctgaca attgctgcct gtgcggcaac ggcggcggcg atcctcggga
    15421 ctattgcggc ggtggtgctg gtgcgctttg gcaggtttcg cggatcaaat ggctttgcct
    15481 ttatgatcac cgcgccgcta gtaatgccag atgtcatcac gggcttgtcg ctgttgttat
    15541 tattcgtcgc gcttgctcat gccattggct ggcctgcgga ccgcggtatg cttaccatct
    15601 ggctggcgca tgtcactttc tgtacggctt atgtggcggt cgttatttcg tcgcgtctgc
    15661 gggaactgga tcgctcgata gaagaagccg cgatggatct cggtgcgacg ccgctgaaag
    15721 tgtttttcgt cattacgcta ccgatgatca tgcccgcgat catttctggc tggttactgg
    15781 cttttacttt gtcgcttgat gatctggtga tcgccagctt tgtttccggg ccgggagcca
    15841 ccacgttacc gatgctggtc ttttccagcg tgcggatggg ggtgaatccg gaaatcaacg
    15901 ccctggcaac attgatcctc ggcgcggtcg gaattgtcgg atttatcgcc tggtatctga
    15961 tggcgcgcgc agaaaaacag cggatacgcg acatccaacg tgcaagacgt ggctgaagac
    16021 actaaaattt gccaacctgg ctacataatg ccgcgcatgt cgcggcattg ttttcatgga
    16081 agacgaaacg ttgggatttt ttaagaaaac atcttcatct catgctcgcc tgaatgtgcc
    16141 tgcgctggtg caggtggcgg cgctcgccat tattatgatc cgtggcctcg acgtgctgat
    16201 gattttcaat acgctgggcg tgcgcggtat tggcgagttc attcatcgca gcgtacaaac
    16261 ctggagttta acgctggtct ttttaagcag tctggtgctg gttttcattg agatctggtg
    16321 tgcgttttca ctggtgaaag ggcgtcgctg ggcgcgctgg ctatatctgc tgacacaaat
    16381 caccgccgca agttacttgt gggcggcttc gctggggtac ggttatccgg agctgttcag
    16441 cattcccggt gaatcaaaac gtgaaatctt ccatagcctg atgctgcaga agctgccgga
    16501 tatgctcatc ctgatgctgc tgttcgttcc ctcgaccagt cggcggttct tccagttgca
    16561 ataatgtgta taatcgtcgc ccctgatgat gtgaaggtca atgtatgcag tgcgcacttt
    16621 acgacgcggg tcgctgtcgt tcctgtcagt ggataacgca gccgattcca gagcaactct
    16681 ccgctaaaac cgccgatctt aaaaatctgc tcgccgattt tccggttgag gaatggtgcg
    16741 cgccggtgtc aggcccggaa caagggtttc gtaataaagc caaaatggtg gtgagtggta
    16801 gcgttgaaaa aacactgctc ggtatgctgc atcgcgatgg cacgccggaa gacctttgtg
    16861 actgcccgct ttatccagcc tcatttgcgc ccgtttttgc ggcgctaaaa ccgtttatcg
    16921 cccgagcggg gttaacgccc tacaacgtgg cgcgtaagcg tggcgaactg aaatacattc
    16981 tgctgactga aagccagagt gatggcggca tgatgctgcg ctttgtactg cgttctgata
    17041 ccaagctggc gcaactgcgt aaggcgctgc cgtggttaca cgaacaacta ccgcagctga
    17101 aagttattac cgtcaatatt cagccggtac atatggcgat tatggaaggg gagacggaga
    17161 tctacctgac cgaacaacag gcactggcgg agcgttttaa tgacgtaccg ctgtggatcc
    17221 gtccgcaaag tttcttccag actaatccgg cggtcgccag ccagttgtac gccaccgcgc
    17281 gcgactgggt acgacagctg ccggttaaac atatgtggga tctgttctgc ggtgtggggg
    17341 gctttggttt acactgcgcg acgcctgaca tgcagttaac cgggatcgaa attgcgccag
    17401 aggccattgc ctgtgcaaag cagtcagccg ctgaactggg cttaactcgt ttacaatttc
    17461 aggcgctgga ctccactcag tttgccactg ctcaggggga ggtgccggag ctggtgctgg
    17521 ttaacccgcc gcgccgcggc attggtaaac cgctgtgtga ttatctttca acgatggcac
    17581 cgcgttttat tatctactcc agctgtaacg cccaaaccat ggcgaaagat atccgcgaac
    17641 tgccaggtta ccgcatcgaa cgggtacagc tttttgatat gttcccgcac accgcgcact
    17701 atgaagtgtt gacgctgctg gtgaagcaat aaaaaagcca caggtgcggc ttcagattgc
    17761 tgatcaggtg cgctttgttc atgccggatg cggcgtgaac gccttatccg gcctacaaaa
    17821 tcatgctaaa tcaatacatt gcagagttga tgtgggccgg ataagcgcag cgcatcaggc
    17881 aatcttactt ttgtcatcag tctcaattac tgcggaaacc actggtcatt gattttctga
    17941 tatgtaccgt cagctttaat tgctgccagc gcgttattca gtttttccag cagagctttg
    18001 ttatccggac gtacagcgat acccaggccg gtgccgaagt attgcggatc ggtcactttt
    18061 tcagtcgcaa cgcccagttg cggattggtt ttcagccatt cgtttaccac cgcggtgtca
    18121 ccaaataccc catcaatacg accatttttc agatcgataa aggcattctg ataactgtca
    18181 taggagacag ttttcacttc cgggtgctga tcctgaatat atttctggtg cgtcgtaccg
    18241 ttttccatcc caatgcgttt gcctttcagg tcggcaaacg ttttgtaggt atcttttttg
    18301 gaaatcacga cggctgagtt ttcatagtag ggtgtggtaa acgaaacctg cttgctacgc
    18361 tccggggtga tatccatacc ggagattacg gcgtcatatt ttctgaattt cagtgacggg
    18421 atcaggctgt cgaacgcgtg attagtaaaa gtacattctg cctgcatttg tttgcacaag
    18481 gcttttgcca gatcgatatc aaagccgaca atctcattat tagcacctat agattcaaag
    18541 ggtggatagg tggctgaaac gccaaaattg attttctctg cggcagaagc accgaaagta
    18601 aaggaagcaa gtaaagcggc aagaactaac tttttcatga tggaactccc gtctgtcaat
    18661 cttatgattt ttggccgtgt ctgcggcatg ggataacaat gccatcaagt gaatttatat
    18721 gcaataaaca tgattaaata atttaaatga aataaaaaaa gacggataac ttagtgagtt
    18781 gtccgtcttc attataagaa tttatgcact atgtaggccg gataaggcgt ccacgccgca
    18841 tccggcacag gcaccgtgct gatgtctgat gcgacgctgg cgcgtcttat cagacctaca
    18901 aacccccccg gcgaatgtac gcagctacat taattccgcc gttcgaatgc cagtgctttg
    18961 cgctcgatca gacgcatcat cagcgtcagc aggccgttga cgaccaagta aataatccct
    19021 gccgcaccga acaccattac atcgtaggtg cgtccgtaca acaactggct gtatcccatc
    19081 acttccatca gcgtaatggt gtaagccaga gaggtacttt tgaacaccag caccacttcg
    19141 ttggaataag aagagagcga gcgtttaaag gcatacggca gcaggatcgc cagcgtatct
    19201 tttttgctca ttcccagggc gctacaggac tgccactgac cttctgggat cgcacgaatt
    19261 gcaccgtaaa acagctgcgt ggtatacgcc gcactgttca gcgataacgc aatcagcgca
    19321 cataaccacg gttctgacaa caaatgccac agtgccggat actcctgcaa cgtcggaaac
    19381 tggcccggcc cgtaataaat caggaagatc tgcaccagca gcggcgtacc ggtaaacagc
    19441 gtgatataac cccgcaccag ccacaccagc accggcgttt tcagcgtcag gatgatggta
    19501 aaaatcaatg ccagaatcag tgccacaatc agcgaggcaa cggttagcgt caggctggtg
    19561 tgcagccctt tcatcagctc gggtaaatac tcaaacatta gctgggcctc cgctcaaaac
    19621 gtgtcgcgcg caggtcaatg cgtttgagaa tgtactgact gagcagggtg atcaccaggt
    19681 aaatcgccgc cgccacaatg taccaggtaa atggttcctg ggtacgagta gcaatgcttt
    19741 tggtttgtaa cattaaatca ttcacactaa tcaaactgac cagcgcggta tctttcagca
    19801 gcaccagcca ctggttaccg aggccaggca gcgcatgacg ccacatctgc ggcatcacca
    19861 gacggaaaaa gatagccgat ttcgacagcc ccagcgcctg accagattcc cactggccca
    19921 ccggtacagc tttcaacgcg ccgcgcagcg tttgcgaggc ataggcggca tacagcagtg
    19981 acagagcgat gacaccacaa aggaacggac tcacgtcgaa gttctcaatg tccatctgca
    20041 ccgggatctg cacgaaccca agattgatag tgaagccatc cgaaagcgtc agcagtaact
    20101 gcgaggagcc aaaatagata aacagcacta ccagaatttc tggcaggcca cgcagaatgg
    20161 ttaccagcgc tgaacctgcc cacgcgacag gacgccattt cgctgactcc cataccgcaa
    20221 agaacatcgc cagcgccagc ccgacaatca atgcacaaac ggcaaggccg acggtcatcc
    20281 cggcggcgct tgctaaagga aaaaattcat tcatcaggaa ttacttctgg aaccatttgt
    20341 tgtagatggt ttcgtaagtg ccatctttct tcactttttc cagcgcagtg ttgagtttct
    20401 gctgcagttc agtgtttccc tgacgtaccg cgatgccgag gccagtaccg aaataatctt
    20461 tatcggtcac tttatcgccc actgccgcca gttttgggtt atctttcagc cactcagtga
    20521 ccactgcggt gtcaccaaat acgctgtcga tacgcccgtt ttgcagatcc agttttgcgt
    20581 tctggtagct gtcatacgga acggtgatga tttccgggtg cttatccata atgaacttct
    20641 ggtgtgtcgt cccgttctga acgccgactt ttttgccttt cagctgatca acactggtgt
    20701 atttgccttg ctgacccaca aacagggcag agttgtcata gtacggggta gtaaacagta
    20761 cctgcttttc acgctccgga gtgatatcca tgcccgccat cactgcttct acgcgacgga
    20821 atttcaggct tgggatcagg ctgtcaaacg cctggttaga gaaagtacag gttgcatcaa
    20881 tctctttaca cagtgcctgc gccaggtcga cgtcaaaacc aacgatctgg ttgtttgcat
    20941 caatcgattc aaacggagga tagggggctt cggtagcaaa acgaatggtt tcggcagctg
    21001 tggcggaaag actaaaacct gcaattaacg cggcaatcag aacttttttc attgttgtta
    21061 tcccgaatct tagtgagaga gatagttttt aaatgcttcg gtttgcggct cggtaaagca
    21121 gctcgcgtcg ccttgttcta cgatatgacc attttccata tacaccactc ggctggcggt
    21181 tttacgcgcc acttcaactt cgtgggtgac gatcacctgg gtaatattcg tttctgccag
    21241 ctcacgaatg atgctgacga tttgtgccgt aatttccggg tccagtgcgg cggtcggttc
    21301 atcgaacagc agtacctgcg gttccatcat caacgcacgg gcaatagcaa cacgctgctg
    21361 ctgaccacca gaaagatgca gcgggtaacg atcgctataa ggtttgagac gcagacgttc
    21421 cagcagtttt tctgcacggg ccagcgcctg atctttactc aaccccagta cacggcaggg
    21481 cgcttcaatc aggttttgct gcacggtcag atgcggccac aggttgtatt gctgaaacac
    21541 catgccaacg ttacgacgca aatcgcgaat cgctttgtca gagggtgttt tggtgaaatc
    21601 gaaatggttg cctgcaatgt tgagcgtacc ggagcgcggc atctcaagca gattgagtac
    21661 acgcagcagc gagcttttac ccgcgccgct ggggccaagt aacaccagcg tttcgccctg
    21721 tgggcaatcc agcgtgatat cgaacagcgc ctgatgcgcg ccgtagaagc aattaatgcc
    21781 gtttaattga atactcattg acactcgtat actggcagtc tgatagctat tgaggtcgaa
    21841 gatagtacct ttaacagaat aattatgcaa tatttatgct ttaaaagtta aaagcaaagc
    21901 gcattattca ataaacatag cacaaaataa cgggggcggt ggtcggtgag cataaatgtc
    21961 ggcattcctc acgaaatgcc ggataattta cggggtttat tggttgatca aggcgttagc
    22021 gattctcgat ggactgacgg agcgtacccg ccgtggcatg aacgctaccg cctaagtagc
    22081 gcacatcgtc gatgacccaa cactggcctt cctgaatcat taacacttca tcctgccagc
    22141 cctggtcacc ctgtttgaga tccacccgca atggaatgtt acgggcatca cgattaggga
    22201 tagtcgatgc actggcaacg tgggcgctgt ctggcaaggt ggttcgactg gagaacggat
    22261 cgctgcttaa caattcacga tgactattat cgcggctggc atcgctaagt tgggttgcca
    22321 gtttgtcact caaatagggg cgcagggcgg taatgtcatt gctgcgatgt tgaatgcggt
    22381 agtcgtagaa ttgttgagcc acactgtcag gccctccctg gacacaggaa ccggtgcggg
    22441 ggccgttatc tttataagct ggcgtgacgg tggtacaggc actcagcagc agtgcacatg
    22501 gaataagtag agatagtgaa cggtagcgca taatcatttc cttattagct tgctgtctgc
    22561 gtcacactac gaccaaacgc tatcagatta tttaatacat tgtttttaat acaattctga
    22621 aacaatctga acacctgatt gggtttgagt ccattgctgg cgacccactc aaggttgctc
    22681 gtagctgact tacttaaatt acccaaacaa tagacagtgt aaaccatttg tgaggttgag
    22741 cgtgttatat ggctgaaatg atcatttaag gtaaatatga tagttctgaa atggtggtat
    22801 aaatcctcct gtagaggtta agagcatctg caagaagcca gtttcacatt tataacgcgt
    22861 gataaatgaa tgcggttatt gtctaatcat tacaaaattt ttcacatgcg ataagagatc
    22921 ctgcgattaa gatgattgcc aaaattacgt tgtatttttg aaaatgagcg aaatttgata
    22981 gtcccagctt tgactctcga taatattatt tatgctacaa ttgtgggtaa agaatttaag
    23041 ctaatgaatt tgagtgaaga ataaattaaa ttgcttcagt atttcgttaa ttaatataaa
    23101 tacaaaaggc gttgtgcttt tataattata aagcgcgagg agttcggtgg attatcgttt
    23161 ttcttgggtg atttttaata tgataaacaa tatatctgat caagcatcat catttccagg
    23221 cacacaacta aatcaaagtg ataatttcct ggattcactg agggaatttt tcgcgatatt
    23281 aaatcccagt cgtaagggtg agttgtctac atgggatacc atctacttgc atctgatatt
    23341 agcaataaat gctgacagtg atctcataaa aaatgatgtg ttgctcgcgg aaaatattcc
    23401 atcagcgaat taccaattta atacattttt tagcaatact ttcgagattg atgttaaaaa
    23461 atatctgggt aaatcagaag ataacgaagt tgaaataaaa gcgggtaatg aaagaatctc
    23521 tattggaata cgtaatgtat cgaatggaaa acttgaaaga caacagtttc ttttcccact
    23581 ggactatgag aataaattac aagaacaatt agataagtat tttacgattg aatcgcaccc
    23641 attattatac aggtatacca ttggtagtaa gattgcgaat gtgatttttg aaaagttata
    23701 cagtcgtatt gactttaaca aagaacaata tatatccttt ataaaggacg catttattca
    23761 tttttatgat tatagcaggc gatatgctat tagtgagaat atcgataaag acgctgtaac
    23821 aaacaatatc gcattgatga gcacttttta tgacagtgat aatacgtcag gtgaggtttt
    23881 aaataatgat tttacagaag aagagtcatt tgaaactgcg ttagatgtag agcatgcaat
    23941 agtgcttgga tttgctgatg ataattttga aacaaaacct gttcattatc aggatttatt
    24001 aacacgattt agtgcctttc aagataccgt gtttaatctg tttcctgaaa tgcactcttc
    24061 gcattaccac gatatttgta gcgtaagtgt ggatatgaca aagggaacgc aatgcatgat
    24121 tcacttgatg gtgaatgaag aagttttcat gtctctgcct gttccagttg ccacgatggt
    24181 acgtgaagat gcaagtaatt tagtcaatct taaaacacta ctcaatgatg gttgtttcat
    24241 taaatatagt cattttaatg acgtggcatt aataaagcaa aacataagta atctctattt
    24301 atcacacact gttgtgaatg aaagcatatt gaaaaaatgt tgttttgaaa acggcagttt
    24361 aggtgatgtt aaaataacaa atagcaatgt aataaacagt gcattcaaaa atatttcgtt
    24421 tagatctgtt aaaattaata acgtaaatac tcattcttta aaatttatta actgcaattt
    24481 tttcaatgtt gatatgataa gagttaatct cagcaaatgt ctttttcatg aatgtagtat
    24541 gcatggtgtc aagataaaac cctggttgcc tgtaaaatgg acaaaagaat taataagtga
    24601 ttatttgtat ggctgtttac tatctctgta ctctatctgt gcaagggata tatataatat
    24661 gaacgccggg aataacgtga aggttgctgc ggatgctttc cttgaaatta ttttctctct
    24721 taaaaataaa tattgtatta aactattaag cgctcaggat cgggcgttta tatacgagtt
    24781 tgcaagaatg atcttcgctt atattaatga caaaagcatt gagatcttat tactttcttg
    24841 ttttgcggcg gctgaccaaa aagcaatcca gcggtataga cctcagtcac aagatgggga
    24901 agactttagg agtcatttac aatataagtt gcctctttcc gctcattaat gttgttggtg
    24961 caacataata agacaggtaa gcggtaccct ataatgaaac caatatcggg ttatgcaacg
    25021 gataatcgta aagacttaag tgggtgtttt tgacacaatg agatgatttc ctaaactgat
    25081 caaaaccttc ttcccaacat ggcatatcag tccgataatg ctttagagat cgaaggctta
    25141 acaaacaagg agatcgatca tgcaattttc tacaacccca actctggaag gcctgaccat
    25201 cgttgaatat tgcggtgtgg tgaccggcga agcgatttta ggtgccaata ttttccgtga
    25261 tttctttgcc ggtatccgcg atatcgttgg cggacgttcc ggtgcgtatg aaaaagaact
    25321 gcgtaaagca cgggagatcg cctttgagga attaggctcc caggcgcggg cgctgggggc
    25381 cgatgccgta gttggtattg atatcgacta cgaaacggtc gggcaaaacg gcagtatgct
    25441 gatggttagc gtcagcggta cggcggtgaa aacgcgtcga tgagaagagt cttctggctg
    25501 gtcgctgccg ctctgttact ggcagggtgt gcaggcgaaa aaggcattgt cgagaaagag
    25561 ggatatcagc ttgatacccg acaccaggcg caggcggcgt atccgcgcat taaagtgctg
    25621 gtgatccact acaccgcaga tgattttgat agctcgctgg cgacactgac tgataagcag
    25681 gtcagctcgc attatctgat ccctgcggtg ccaccgcgat acaacggtaa accgcgcatc
    25741 tggcaactgg tgccggaaca agaactggcc tggcatgcgg ggattagcgc ctggcgcggg
    25801 gcaacgcgcc ttaacgacac ctctattggc attgagctgg aaaaccgtgg ctggcaaaaa
    25861 tcatccggag tgaaatattt tgccccgttt gaacaggcac agattcaggc gcttattccg
    25921 ctggcgaaag atattattgc ccgttatcac atcaagccgg aaaacgtagt ggcacatgcg
    25981 gatatcgcac cgcagcgcaa agacgatccg gggccattat ttccctggca gcaactggcg
    26041 cagcagggga ttggtgcctg gccggatgcg cagcgggtga acttttacct tgccgggcgc
    26101 gcgccgcaca caccggtaga taccgcgtca ttgctggagc ttttggcgcg ctacggttat
    26161 gacgtaaaac ctgatatgac accgcgcgag cagcggcgcg tgattatggc attccagatg
    26221 catttccgcc cgacgttata taacggcgaa gcggatgcag aaactcaggc gattgccgaa
    26281 gcattgctgg agaaatacgg gcaggattag cgcggcagtt ttccgtggtc gcgtagccag
    26341 gcggcagttt tctcgatgcc ttcatccagg gtgatgaccg gctgataacc taactcttcc
    26401 tgcgcacgcg taatatccag cgtaaagtca aaattcaact tggaggcgcc gtagtgggtc
    26461 agcggcggct cttttgctga cttgcggcct aaacgctcca tgctgcgggc gatcatatcc
    26521 agcatcgggt aagggacgga acgaatacga cagtcaatat tcaactcgtc gatcagcttc
    26581 tgcacgatgc tgcgcagtgt gcgatgctcg ccgttggtga tgttgtacac acgcccggaa
    26641 ggtagcttat cgcaggcttc ctggcttgcc agccacattg cgtgcacggc attttcatag
    26701 taggtcatat cgaccagcgc actgccgcca tgcggtaaca gaatactgcc gtagtggtgc
    26761 atcatatgcg ccagacgggg aataaagact ttatcgtgcg gtccgaacag actttgtggg
    26821 cgcagaatag taaagcgcgt ttgtggattc gcctgcgaaa gcatattgat cacttcttcg
    26881 ctggctgctt tgctgcgggc aaactcgttg gcgaagcggt gagggcgaaa atcttcttta
    26941 atattgcgat ggtggtgata atcgaagtac agggaggggg aagagatatg aataaagtta
    27001 cgcacacccc aggcgacagc ccattcaccc aggcggcgag tggcgcgaac gttagccaga
    27061 tcgaaagcct gttgtgtccc ccagggtgag gtaaagctgg agcagtgcca cagcgtatca
    27121 atgcccgcga gcatcacttt agcttgtgat gaaaccagct cggtcagatc cgtcggaaca
    27181 aactctgcgc ccattttttc cagcaattta cccattgctt cgttgcgacc gctcgcacgc
    27241 acgctgatgc ctttctggca taaaaactct accgcgtttc gacctaagcc gctggtggcg
    27301 ccggtaacca gtaccttcat atcaatccac tgttgttgag aaaatgacgt gcgcattctt
    27361 ccgtgatttc ccccatgatg caatgggaaa catgaaagaa taacgcaggt tttgtcgatt
    27421 aatctgtgct ttgttctgcc agtctggcga tttgttttgc cattccgcga aaaataaaca
    27481 gatgcgcggg gatcatcaat aaccagtaaa acagccccgg cataccgtgc ggatgccaga
    27541 aagcgcggac atcgatagta cgatagtcac ctttatcttc cagggtaaaa cacagtcgtc
    27601 ccagccccgg cgctttcatg ccaaataaca acgtaagttg ctttttcggt tcaacgacaa
    27661 tcactttcca gctatccacc gcatcgccgg tctgtaaata ttcgcgctcc gggcggcctt
    27721 tcgccagatt atgaccgatc gcgcggtcca tcaacgcccg tgtctgccac aaaatattgc
    27781 caaagaaata acgctcttta ccgccgattt ggttcaccac cagccataaa gccgcaaggc
    27841 tggcggacgt tttaacggta aaccccgcct gtttggcgaa ataaccgtac tccggtcgcc
    27901 agcgggcaaa ggcctgagcg tcgtagcccc agtcgctgga gttgaccagt ttttcttcct
    27961 ctttcaacgt gctacgtacc gcgtcatcga aagcgatcag ccgttgtggg atgagtgcac
    28021 gtagcgcggt atcatctgcc agcagatcgt gtttcagccc ctgaatcaac gccctggcgg
    28081 tggtgggcgg tacggaagta atcacattga gaaaccacac cgaaatccag cgggtgggga
    28141 gggggatggg gatcaaccag cggcgcttac cgctcaccgc cataaaatgt tcaaactgtt
    28201 gctgataact gagcacctct ggtccggcgg cttcgaagat gcggtgttcg ttggctggat
    28261 ggtctaacag cgccaccaga tagtgcagca agttttccag cgcgatgggc gtggtgcgtg
    28321 aacgtaccca gcgtggcggc gttaacactg gcaggttgta gaccatatcg cgcatgactt
    28381 cgaacgccgc tgaacctgcg ccaacgataa ttccggcccg aagttcggtc actggtacat
    28441 tcgcttcacg aagaatgtcc gccgtagcct gacgagctcg cagatgatcc gactgctcat
    28501 gtggcggggc ctgcaacgaa ctgagaaaga ttaattgctt aactggtact tcacgtagcg
    28561 catcgcggac gttgagagcc agctggcgct cctgagcgat aaaatcgccg ccttcgccca
    28621 tgctgtgcac cagaaaatag acggtatcga tatcctgcaa cagggccgga aggttatccg
    28681 gccagctgag atcgacttta tggcaactga cgtttgccag ttgcagcttt gcaagcctgt
    28741 cgacatgacg tgccgccgct aggatctgat gcccttgctg gctgagtgtg cgcaccagat
    28801 gctgaccaat gtagccactg gcaccgagaa ctaaaatgcg ttgcggcacg tctctctcct
    28861 taacgcgcca ggaatgcgcg ccagtgggcg gcgacttccg ccagttgttc gcgcgagacg
    28921 tcaagatgcg tcaccaggcg gacaatcggc gaggcgttaa tcagcacgtt tctcgctttc
    28981 atgtattcgc ctaacgcggc agcattttct tccccgacgc gaacaaacag catattagtg
    29041 tcctgacgca tcacatccgc gcctgcttca cgcagttgct ccgccatcca ggcggcgttg
    29101 tcgtgatctt cctgcaaccg tgcgacgtta ttttccaggg catacatccc ggcggcagcc
    29161 agaatcccgg actgacgcat cccgccaccg gccattttcc gccagcggat ggcacgttta
    29221 atgtaatcac gattaccgac gagtaatgaa ccgactggcg tcccaagacc tttcgacagg
    29281 caaatggtga acgaatcaca atattgcgtg atctctttca gttcgcagcc gtaagccacc
    29341 acggcattaa agatgcgcgc accgtcaaca tgcagcgcca gattgcgctc gcgggtaaat
    29401 tcccatgctt ctttcaggta ttcacgcggc agcactttgc cgttgtgggt gttttccaga
    29461 ctgagtaatt tggtgcgggc gaaatggata tcgtcgggtt tgattttcat cgccacttta
    29521 tccagcggta gcgtgccgtc ggcagccgcg tcgatgggtt gcggctgaat actgcccagc
    29581 accgctgcgc caccggcttc aaacagatag ttatgcgcgg cctgaccgac aatatactct
    29641 tcgccacgtt cgcagtgact gagcagagcg accaggttgg cctgagtgcc ggtaggcaga
    29701 aaaatggcgg cttctttacc ggaaagctct gctgcatagt cctgcagagc attaacggta
    29761 gggtcgtctc cgtaaacgtc gtccccaacc ggggcggcca tcatcgcttc gagcatggcg
    29821 cggctcggtc gggtaacggt atcactgcgt aaatcaatca tggcatgtcc ttattatgac
    29881 gggaaatgcc acccttttta ccttagccag ttcgttttcg ccagttcgat cacttcatcg
    29941 ccgcgtccgc tgataattgc gcgcaacata tacaggctaa aacctttggc ctgttcgagt
    30001 ttgatctgcg gtggaatggc taattcttct ttggcgacca ccacatccac caacaccgga
    30061 ccgtcgatag agaaggcgcg ttgcagggct tcatcaactt cagaggcttt ttctacacgg
    30121 atacccgtaa tgccgcacgc ttcggcaatg cgggcaaagt tggtgtcgtg cagctcagta
    30181 ccgtctgtca ggtaacctcc ggccttcatc tccatcgcca caaagcccag cacgctgtta
    30241 ttaaagacga taattttcac cggcagtttc atctgcacta ctgagaggaa atcgcccatc
    30301 aacatgctaa aaccgccatc gccgcacatg gcgaccacct gacgttccgg ctcggttgcc
    30361 tgcgcaccca acgcctgcgg catggcgtta gccatcgaac cgtggttaaa cgaacctaac
    30421 aggcgacgct tgccgttcat tttcagataa cgtgccgccc ataccgttgg cgtgccgacg
    30481 tcacaggtaa agatggcgtc atcggcggca aaatgactaa tttgctgcgc cagatattgc
    30541 gggtgaatgg ctttctcgct cggtttagct aaatcgtcca gccctttgcg ggcatcgcgg
    30601 taatcttcca gcgctttatc cagaaacttg cgatcggttt tttcttccac cagtggcagc
    30661 aatgcgcgca gggttgattt gatatcgccg accagcgcca tatctacctt gctatgcgcg
    30721 ccgatgctgg ctggattaat atcaatctga ataattttgg catcggtcgg gtagaacgca
    30781 cggtagggga actgagtacc gagtagcact aacgtgtcgg cgttcatcat ggtatggaaa
    30841 cctgacgaga atccgattaa cccggtcatt ccaacatcat acggattatc gtattcgaca
    30901 tgctctttac cgcgtagggc atgaacaata ggtgctttaa ttttcccggc aaactcaact
    30961 aactctttat gcgcccccgc gcagccgctg ccacacatca gggcgatatt gctggaataa
    31021 cgcagcagtt gcgccagttt gcgtaactct tcttcttccg gcatcacgac tggctgtggc
    31081 gcatgatacc agtgcgtggt tgccccttct ggcgcaggtt ttaacgccac gtcgcccggt
    31141 aatacaacaa cggaaacgcc acggttaagc accgctttgc gcatagcaat tgccagcact
    31201 tgtgggatct gctccgggct ggaaaccagc tcgcaatagt gactacattc gcggaatagc
    31261 tcttgtgggt gggtttcctg gaaatagccg ctgccaattt cgctggaggg aatatgagcg
    31321 gcaatcgcca gtaccggaac gttattgcgg tggcaatcga ataggccgtt gatcaagtgc
    31381 aggttgcctg ggccgcacga tccggcacag accgccagtt ctcctgaaag ttgtgcttca
    31441 gcgccagcgg caaacgccgc cacttcttca tggcgggtgg acatccactc gatggtgccc
    31501 atgcgattaa gactgtcgct aagaccgttc agagagtcgc ccgtgactcc ccagatgcgt
    31561 ttcacgcctg ccgattcgag tgttttggcg atataagctg caaccgtttg tttcatggtt
    31621 ctccatctcc tgaatgtgat aacggtaaca agtttagttc atctgacgga gggggaaggg
    31681 atgggagaga aaggaggcac taacggttaa atagcccgat gaaaggaata tcatcgggca
    31741 taaggcgatt atgcgagaac caaatccccc tgcggatggc aggagcaggc cagtacgtaa
    31801 ccttcagcga tttcggcgtc ggtcagcgtc attgtactgc tcaccgtata ttcaccggaa
    31861 accacttttg tcttacagca gccgcaaaca cccgcacggc aggcagcgac aaccggaacg
    31921 ttattgcttt ccagcgcctc cagtagcgtg gtgccaaccg gggcgtaaaa ttctcgtgcc
    31981 ggttgcagtt tggtgaattt cagaccgctg gtcgccgctt ctgctactgg ggtgaagaat
    32041 ttctctttaa agaaacgcgt cacgccgagc gctttcactt cctgctctac ccaatccata
    32101 tacggagccg ggccgcaggt catcacggta cgtgaagcta agtcaggtac atctgccagc
    32161 agttcgcgag tgaggcgacc agcgataaag ccttcggtaa cgttattttc tgccaccagc
    32221 gttaccggat agttacgcca ctcatcggcg aaaataacat cctgcggcgt acgcacgttg
    32281 tagatcaccc gcacatcggc ctgtggacgg ttcttcgcaa gccagcgacg catcgacata
    32341 atcggcgtga cgccgcagcc tgccgccagc aacaggaatt tatcttctgc tttatcgtcg
    32401 caggtaaatt cccccatcgc gtccgaaagc cagagataat caccgcgttt tacatcgcgc
    32461 gtcagccact gggagccgac accgtcatca atccgccgca cggtcagggt gatatattca
    32521 ctcacgcctg gcgtggagga aatggtgtaa gcacgcagcg tttccgctga gtttcgcacg
    32581 ctgaccagtg catattgccc ggcgcgatat gggtagtgat cgtggcaaat cagggaaatc
    32641 gtccacacat ccggcgtttc ttgcgtaatg tgatgaacct gtatccgcca cgggcattga
    32701 ttcgttggca tcgtcatcga caaactcctt acgcgttcaa cagttgcttc atgtcttctt
    32761 caacagtggt gatagaacgc aggccgaatt tctcgttcag caccgccagc aggtctggtg
    32821 tcaggaaacc aggtgcagtc gggccggtga cgatattttt cacgcccaga gaaagcagcg
    32881 tcagcagaat gacaatcgct ttctgttcaa accaggagag caccagcgac agcggcagat
    32941 cgttcacacc acagcccagt ttctctgcca gagtgacagc cagaataatt gctgagtaag
    33001 catcgttaca ttgacctgca tctaccagac gcggcagacc ttcgatatcg ccaaactcaa
    33061 gtttgttaaa gcgatattta ccacaggcga gggtcaggat caggcagtca tccggcacgc
    33121 tggtggcgaa atcggtgaag tagtggcgct cgccgcgtgc gccgtcacag ccaccaagca
    33181 ggaagatatg acgcagtttt tcacggctaa ccagatcaat cagcgtatcg gctgcaccaa
    33241 gcagcgtctg gcgaccaaag cccacggtga tcaggtgagg aatttcgctg tacgggaagc
    33301 ccgccatttg ttgcgcctgg gcgatgaccg cagagaaatc ttcgccatcc agatgacgca
    33361 cgccaggcca gccaacgatg ctgcgggtcc agatacgatc gtcataagcg cctacggttg
    33421 ggtcgatgat gcagttcgag gtcatcacga tggggccagg gaaacgagcg aactccactt
    33481 gctgattctg ccagccgctg ccgtagttac cgaccagatg cttgaattta cgcagctccg
    33541 ggtagccatg cgcaggcagc atttcgccgt gggtgtagac attaacgccc gtgccttcgg
    33601 tctgttccag caggttgtag agatctttga gatcgtgacc ggaaatcaga atgcatttac
    33661 ccgccgtcgc tttgacgttg acctgggttg gcgtcgggtg accgtattta ccggtttcgc
    33721 ctgcatccag aatgctcatc actttgaagt tcatctggcc gatttccatt gaacactcaa
    33781 gaagcgcgtt catatcggca ggccaggtcc ccagccacgc catgatttta tggtactggg
    33841 cataaatatc gttgtcgtat tgaccgagaa catgcgcgtg ttccatatag gccgccgcac
    33901 ctttcaggcc atacaggcac agcagacgca ggccgagaat gttttcgcca atcgccgctt
    33961 tatctttgtt aggggtaaat tctgctgcct gacgttgcag ctcgccgaga tcatcgctca
    34021 ccagttgcag gtcagccatc gggttatcga cgcgcgcgtt ggcatctaca gccaggcatt
    34081 gtgctttcag cgcctcgcgc aaggcaatcg cttcacgagc gtagccgaca atacgcggag
    34141 aatcgaagtt aacgttggtc agggttgaga aaaaggcacg tggcgcgaag ctgtcaacat
    34201 cgtggttgat gatgccgtat tcacgcgctt ttaccgccca ggcagaaagc ccttgcagcg
    34261 ccgcgatgag taaatcctga aggtcagaag tttccgccgt tttaccacac atcccctgcg
    34321 cgtatgagca gccgttttct gccggagtac ggatagtttg ttcacattgc acacaaaaca
    34381 tgatcacacc ttttaaagtt atatttaata tacatgttta agggtaagac gcttaacgcg
    34441 gggataaaag ggatttttca tgcaacttta agggagattg atttagcgca attttggcgg
    34501 cagggaacta ccgccagaga ggtattacgc agagaaaaag gcgatgagga tcggcactaa
    34561 caggctaaga ataaaaccgt gaacaattgc cgccgggacc atatccagcc cgccagtacg
    34621 ttgaagaacg ggcagggtga aatccattga tgtggcaccg cataagccca gtgcagtaga
    34681 gcggctgcgg cgaatcagcc cagggatcaa cataatagca atcagttcac gggccagatc
    34741 attaaaaaac gccgcgctcc cgattaccgg accaaaagat tcggtcaata aaataccgga
    34801 aagagaatac cagccgaaac cggaggccat tgccagcgcg gtattgatgg ggagatcaag
    34861 aataaaggcg ttaattaaac caccaattaa tgaactgaca accaccacca cggcgacaat
    34921 cattccccgg cgattaagga caatctgctt taaggtcatg ccattattgc gcaactgaat
    34981 accaacgagg aaaagtagca aaattaacgt gtattcactg gcttcggtcg cgtgttgtaa
    35041 gaaagccagt ccactcagac caatggcaaa accaatcact actacgccgc acagttttag
    35101 cgactccagc gccatcgcaa tacgcgacgg gagtttttct tgctgatggt ggttgcgcca
    35161 cggcaggcct cgctccagcc acatcagggc ggcaatatta cacagtaaaa taacggtaat
    35221 actgacggca gaataatgca gaatcgccaa caggttactg gcgaggttat cgagaaacgc
    35281 cagactgata cccataaaaa agagaataag gtaaaccatc cagcttaata gctgattaat
    35341 agcttttaac gcagcttgtt ggcgaagcgg aatgaggtaa cccacaatca ggggaaccag
    35401 aatgattaac agcccagaaa acatgaaaat ccagtccttg caaagaagaa gtcgaaatgc
    35461 gcgatgacac actactgaaa gcggaagggc gagtaaagtt gcaattaaaa ggaaatgtta
    35521 tgcatcagag gggga
//
LOCUS       DAJTQQ010000034        34889 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000034 DAJTQQ010000000
VERSION     DAJTQQ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.034"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..705)
                     /locus_tag="OO309_001844"
     CDS             complement(<1..705)
                     /locus_tag="OO309_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5847004.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQL"
     gene            797..883
                     /locus_tag="OO309_001845"
                     /pseudo
     CDS             797..883
                     /locus_tag="OO309_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1288..1764)
                     /locus_tag="OO309_001846"
     CDS             complement(1288..1764)
                     /locus_tag="OO309_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR5847005.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1823..3112)
                     /gene="bioA"
                     /locus_tag="OO309_001847"
     CDS             complement(1823..3112)
                     /gene="bioA"
                     /locus_tag="OO309_001847"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR5847006.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3199..4239
                     /gene="bioB"
                     /locus_tag="OO309_001848"
     CDS             3199..4239
                     /gene="bioB"
                     /locus_tag="OO309_001848"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR5847007.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4236..5390
                     /gene="bioF"
                     /locus_tag="OO309_001849"
     CDS             4236..5390
                     /gene="bioF"
                     /locus_tag="OO309_001849"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR5847008.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5377..6132
                     /gene="bioC"
                     /locus_tag="OO309_001850"
     CDS             5377..6132
                     /gene="bioC"
                     /locus_tag="OO309_001850"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR5847009.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6125..6802
                     /gene="bioD"
                     /locus_tag="OO309_001851"
     CDS             6125..6802
                     /gene="bioD"
                     /locus_tag="OO309_001851"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5847010.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7381..9402
                     /gene="uvrB"
                     /locus_tag="OO309_001852"
     CDS             7381..9402
                     /gene="uvrB"
                     /locus_tag="OO309_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR5847011.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9594..10502)
                     /gene="yvcK"
                     /locus_tag="OO309_001853"
     CDS             complement(9594..10502)
                     /gene="yvcK"
                     /locus_tag="OO309_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR5847012.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10767..10911
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10899..11888
                     /gene="moaA"
                     /locus_tag="OO309_001854"
     CDS             10899..11888
                     /gene="moaA"
                     /locus_tag="OO309_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR5847013.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            11910..12422
                     /gene="moaB"
                     /locus_tag="OO309_001855"
     CDS             11910..12422
                     /gene="moaB"
                     /locus_tag="OO309_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR5847014.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12425..12910
                     /gene="moaC"
                     /locus_tag="OO309_001856"
     CDS             12425..12910
                     /gene="moaC"
                     /locus_tag="OO309_001856"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR5847015.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            12903..13148
                     /gene="moaD"
                     /locus_tag="OO309_001857"
     CDS             12903..13148
                     /gene="moaD"
                     /locus_tag="OO309_001857"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR5847016.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13150..13602
                     /gene="moaE"
                     /locus_tag="OO309_001858"
     CDS             13150..13602
                     /gene="moaE"
                     /locus_tag="OO309_001858"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR5847017.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13739..14443
                     /locus_tag="OO309_001859"
     CDS             13739..14443
                     /locus_tag="OO309_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5847018.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14648..15361
                     /locus_tag="OO309_001860"
     CDS             14648..15361
                     /locus_tag="OO309_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5847019.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15397..16353)
                     /locus_tag="OO309_001861"
     CDS             complement(15397..16353)
                     /locus_tag="OO309_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR5847020.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16353..17594)
                     /gene="clsB"
                     /locus_tag="OO309_001862"
     CDS             complement(16353..17594)
                     /gene="clsB"
                     /locus_tag="OO309_001862"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR5847021.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17591..18352)
                     /locus_tag="OO309_001863"
     CDS             complement(17591..18352)
                     /locus_tag="OO309_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5847022.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18485..18895
                     /locus_tag="OO309_001864"
     CDS             18485..18895
                     /locus_tag="OO309_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR5847023.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18857..19963)
                     /locus_tag="OO309_001865"
     CDS             complement(18857..19963)
                     /locus_tag="OO309_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5847024.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19974..21107)
                     /locus_tag="OO309_001866"
     CDS             complement(19974..21107)
                     /locus_tag="OO309_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5847025.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21100..22836)
                     /locus_tag="OO309_001867"
     CDS             complement(21100..22836)
                     /locus_tag="OO309_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5847026.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22829..23824)
                     /gene="hlyD"
                     /locus_tag="OO309_001868"
     CDS             complement(22829..23824)
                     /gene="hlyD"
                     /locus_tag="OO309_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR5847027.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23827..24498)
                     /gene="cecR"
                     /locus_tag="OO309_001869"
     CDS             complement(23827..24498)
                     /gene="cecR"
                     /locus_tag="OO309_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR5847028.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24727..25914
                     /gene="rhlE"
                     /locus_tag="OO309_001870"
                     /pseudo
     CDS             24727..25914
                     /gene="rhlE"
                     /locus_tag="OO309_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26121..26603)
                     /gene="ybiA"
                     /locus_tag="OO309_001871"
     CDS             complement(26121..26603)
                     /gene="ybiA"
                     /locus_tag="OO309_001871"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR5847029.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26723..28873
                     /gene="dinG"
                     /locus_tag="OO309_001872"
     CDS             26723..28873
                     /gene="dinG"
                     /locus_tag="OO309_001872"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR5847030.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28901..29863
                     /gene="ybiB"
                     /locus_tag="OO309_001873"
     CDS             28901..29863
                     /gene="ybiB"
                     /locus_tag="OO309_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR5847031.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30004..31089
                     /gene="hcxB"
                     /locus_tag="OO309_001874"
     CDS             30004..31089
                     /gene="hcxB"
                     /locus_tag="OO309_001874"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR5847032.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31318..31578)
                     /locus_tag="OO309_001875"
     CDS             complement(31318..31578)
                     /locus_tag="OO309_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5847033.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASPLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31843..32109)
                     /gene="ybiI"
                     /locus_tag="OO309_001876"
     CDS             complement(31843..32109)
                     /gene="ybiI"
                     /locus_tag="OO309_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR5847034.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32183..32860)
                     /gene="ybiX"
                     /locus_tag="OO309_001877"
     CDS             complement(32183..32860)
                     /gene="ybiX"
                     /locus_tag="OO309_001877"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR5847035.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32902..34889)
                     /locus_tag="OO309_001878"
                     /pseudo
     CDS             complement(32902..34889)
                     /locus_tag="OO309_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8263 a         8955 c         9429 g         8242 t
ORIGIN      
        1 cagctgattg ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg
       61 tagttggttt tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg
      121 taatctgtta tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt
      181 aagctggttg ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc
      241 caggctattg agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt
      301 taaaactctg ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct
      361 gctcagtctg ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga
      421 gcgtagctca ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga
      481 gcgtaatctg agtaaactta aattgagggc tggctcttga aaagccagac atattttcag
      541 taatcgaact gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca
      601 gatgcggtga taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa
      661 accacaaaag gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc
      721 attgtttata tattaatata ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc
      781 tgtaaatagt taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt
      841 gaagttctgg ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt
      901 catcaagcca gtagtctgcc caccacagca tcatttctct gcgcttatcg agatactgag
      961 catggttgta aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa
     1021 aatcaccttg cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat
     1081 agtgactgga tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat
     1141 tataatgcat tgatattaat gatttttact gtttctcggt cagctttttt atactaactt
     1201 gagcgaaacg ggaaggtaaa aagacaaaaa gttgttttta atacctttaa gtgataccag
     1261 atggcattgc gccatctggc agagtgatta actaaacatc gcggtaatcg atgcgcttgc
     1321 cagagagtgg aaatgaacgt taaacccgac catcgcgccg ctggctcctt catcgacatc
     1381 aatacgctct acatccagcg cgtgaacggt aaaaatgtag cgatgggttt cgcctttcgg
     1441 cggcgctgcg ccatcgtatc cggttttacc aaaatcggta cgcgtctgca aaacgccgtc
     1501 tggcattgct accagaccag agccaaaccc ttgcggtaat acgcgggtat cagcgggtaa
     1561 attaacaact acccagtgcc accagccgga gccggttggc gcatccgggt cgtagcaggt
     1621 gacaacaaaa cttttcgttc ccgcaggaac atcatcccac gccagatgcg gtgaaatatt
     1681 atcgccatcg taacccatgc cgttaaagac atgacgatgc ggcaacttat cgccatcgcg
     1741 cagatcgtta ctgatgagtt tcatgaaccc tcctttcttg tttgcagaaa gtgtagccag
     1801 aaaccctcac gctgacttcc cgttattggc aaaaaaatgt ttcatcctgt accgcgcggt
     1861 taaccgctgc ggtcagacgc tgcaactgtt gcgggagaat aatatagggc ggcatcaggt
     1921 aaatcagttt gccaaaaggc cggatccata ccccctgttc gacaaagaat ttttgcagcg
     1981 ccgccatatt caccggacga gtggtttcga ccacaccgat tgcccccagt acgcgcacat
     2041 cggcaaccat ttcggcatca cgggctggtg ccagttgctc gcgcagctgc acttcaatag
     2101 ccgccacctg ctgctgccat tcgccggatt cgataatcgc taggctggcg tttgctgccg
     2161 cgcaggccag cggattgccc ataaaagttg gcccatgcat aaagcagccg gcttcgccgt
     2221 tactgatggt ttctgcaacc tcgcgcgtgg taagtgtggc ggaaagggtc attgtgccgc
     2281 cggttaaggc tttaccgagg cacaaaatgt ccggcgcgat ttctgcatat tcacaggcaa
     2341 acagtttgcc ggtacgacca aatccggtgg cgatctcgtc ggcaatcagc aagatacctt
     2401 cgcgatcgca cattttgcgg attcgtttta accattccgg atggtacatg cgcatcccgc
     2461 ctgcgccctg gacaatcggc tcaatgatca ccgctgcgat ttcatgacga tgcgccgcca
     2521 tcaggcgggc aaaaccaacc atatcgcgtt catcccattc accatccatg cggctttgcg
     2581 gggcgggagc aaacaagttt tctggcagat agcctttcca caggctgtgc attgagttgt
     2641 ccggatcgca caccgacatc gcgccaaagg tatcgccatg ataaccattg cggaaggtca
     2701 gaaaacgctg gcgcgcttcg cctttggctt gccagtactg caacgccatt ttcatcgcca
     2761 cttccaccgc tacggaaccg gagtccgcga gaaaaacgca ctccagcggt tgcggcgtca
     2821 ttgccaccag tttgcggcat agctcgatgg cgggcgcatg ggtgataccg ccaaacatca
     2881 catgcgacat ggcatcaatt tgcgtcttca tcgccgcatt aagctgcggg tgattgtagc
     2941 cgtggatcgc cgcccaccag gacgacatac cgtcaaccag gcgtctgccg tcagacaaaa
     3001 ttagctcgca accttcggcg ctcgccaccg gataaaccgg cagaggggag gtcatggatg
     3061 tgtatgggtg ccagatatgg cgttggtcaa aggcaagatc gtccgttgtc ataatcgact
     3121 tgtaaaccaa attgaaaaga tttaggttta caagcctaca ccgaattaac aacaaaaaac
     3181 acgttttgga gaagccccat ggctcaccgc ccacgctgga cattgtcgca agtcacagaa
     3241 ttatttgaaa aaccgttgct ggatctgctg tttgaagcgc agcaggtgca tcgccagcat
     3301 ttcgaacctc gtcaggtgca ggtcagcacg ttgctgtcga ttaagaccgg agcttgtccg
     3361 gaagattgca aatactgccc gcaaagctcg cgctacaaaa ccgggctgga agccgagcgg
     3421 ttgatggaag ttgaacaggt gctggagtcg gcgcgcaaag cgaaagcggc aggatcgacg
     3481 cgcttctgta tgggcgcggc atggaagaat ccccacgaac gcgatatgcc gtacctggaa
     3541 caaatggtgc agggggtaaa agcgatgggg ctggaggcgt gtatgacgct gggcacgttg
     3601 agtgaatctc aggcgcagcg cctcgcgaac gccgggctgg attactacaa ccacaacctg
     3661 gacacctcgc cggagtttta cggcaatatc atcaccacac gcacttatca ggaacgcctc
     3721 gatacgctgg aaaaagtgcg cgatgccggg atcaaagtct gctcaggtgg cattgtgggc
     3781 ttaggcgaaa cggtaaaaga tcgcgccgga ttattgctgc aactggcaaa cctgccgacg
     3841 ccgccggaaa gcgtaccaat caacatgctg gtgaaggtga aaggcacgcc gcttgccgat
     3901 aacgatgacg ttgatgcctt tgattttatt cgcaccattg ccgtagcgcg gatcatgatg
     3961 ccgacctctt acgtgcgcct ttctgccgga cgcgagcaga tgaacgaaca gactcaggcg
     4021 atgtgcttta tggcaggcgc aaactcgatt ttctacggtt gcaaactgct gaccacgccg
     4081 aatccggaag aagataaaga cctgcaactg ttccgcaaac tggggctaaa tccgcagcaa
     4141 actgccgtgc tggcagggga taacgaacaa cagcaacgtc ttggacaggc gctgatgacc
     4201 ccggacaccg acgaatatta caacgcggca gcattatgag ctggcaggag aaaatcaacg
     4261 cggcgctcga tgcgcggcgt gctgccgatg ccctgcgtcg ccgttatccg gtggcgcaag
     4321 gagccggacg ctggctggtg gcggatgatc gccagtatct gaacttttcc agtaacgatt
     4381 atttaggttt aagccatcat ccgcaaatta tccgtgcctg gaagcagggg gcggagcaat
     4441 ttggcatcgg tagcggcggc tccggtcacg tcagcggtta tagcgtggtg catcaggcgc
     4501 tggaagaaga actggccgag tggcttggct attcgcgggc gctgctgttt atctctggtt
     4561 ttgccgcgaa tcaggcagtc attgccgcga tgatggcgaa agaggaccgt attgttgccg
     4621 accggcttag ccatgcctca ttgctggagg ctgccagttt aagcccgtcg cagcttcgcc
     4681 gttttactca taacgatgtc gctcatctgg cgcgactgct tgcttccccc tgtccggggc
     4741 agcaactggt ggtgacagaa ggcgtgttca gcatggacgg cgatagcgcg ccactggcgg
     4801 aaatccagca ggtaacgcaa cagcacaatg gctggttgat ggtcgatgat gcccacggca
     4861 cgggcgttat cggggagcag gggcgcggca gctgctggct gcaaaaggta aaaccagaat
     4921 tgctggtagt gacttttggc aaaggatttg gcgtcagtgg ggctgcggtg ctttgctcaa
     4981 gtacggtggc ggattatctg ctgcaattcg cccgccacct tatctacagc accagtatgc
     5041 cgcccgctca ggcgcaggca ttacgtgcgt cgctggcggt cattcgccgt gatgaggggg
     5101 atgcacggcg cgaaaaactg gtgtcactca ttgcgcgttt tcgtgccgga gtacaggatt
     5161 tgccgtttac gctggctgat tcatgcagcg ccatccagcc attgatcgtc ggtgataaca
     5221 gccgtgcgtt acaactggca gaaaaactgc gccagcaagg ctgctgggtc acggcgattc
     5281 gcccgccaac cgtacccgct ggtactgcgc gactgcgctt aacgctaacc gctgcgcatg
     5341 aaatgcagga tatcgaccgt ctgctggagg tgctgcatgg caacggttaa taaacaagcc
     5401 attgcagcgg catttggtcg ggcagccgca cactatgagc aacatgcaga tctacagcgc
     5461 cagagtgctg acgccttact ggcaatgctt ccacagcgta aatacaccca cgtactggac
     5521 gcgggttgtg gacctggctg gatgagccgc cactggcggg aacgtcacgc gcaggtgacg
     5581 gcgttagatc tctcgccgcc aatgcttgcg caagcccgcc ataaagatgc cgcagatcat
     5641 tatctggcag gagatatcga atccctgccg ttagcgactg cgacgttcga tcttgcatgg
     5701 agcaatctcg cggtgcagtg gtgcggtaat ttatccacgg cactccgcga gctgtatcgg
     5761 gtggtgcgcc ccggaggcgt ggtcgcgttt accacgctgg tgcagggatc gttacccgaa
     5821 ctgcatcagg cgcggcaggc ggtggacgag cgtccgcatg ctaatcgctt tttaccgcca
     5881 gatgaaatcg aagagtcgct gaacggcgtg cgttatcaac atcatattca gctcatcaca
     5941 ctatggtttg atgatgcgct cagtgccatg cgttcgctga aaggcatcgg tgctacgcat
     6001 cttcatgaag ggcgcgaccc gcgaatatta acgcgttcgc agttgcagcg attgcaactg
     6061 gcctggccgc aacaacaggg gcgatatcct ctgacgtatc atcttttttt gggagtgatt
     6121 gctcgtgagt aaacgttatt ttgtcaccgg aacggatacc gaagtgggga aaaccgtcgc
     6181 cagttgtgca cttttacaag ccgcgaaggc agtaggttat cggacggcag gttataaacc
     6241 ggtcgcctct ggcagcgaaa agaccccgga aggcttacgt aatagtgacg cgctggcgtt
     6301 acagcacaac agcagcctgc agctggatta cgcaacggta aatccttaca ccttcgcaga
     6361 atccacctcg ccgcacatca tcagcgccca agagggcagg ccgatagaat cattggtaat
     6421 gagcgccgga ttacgcgcgc ttgaacaaca ggctgactgg gtgttagtgg aaggtgctgg
     6481 cggctggttt acgccgcttt ctgacacttt cacttttgca gattgggtaa cacaggaaca
     6541 actgccggtg atactggtag ttggtgtgaa actcggctgt attaatcacg cgatgttgac
     6601 tgcgcaggca atacaacacg ccggactgac tctggcgggt tgggtggcga acgatgttac
     6661 gcctccggga aaacgtcacg ctgaatatat gaccacgctc acccgcatga ttcccgcgcc
     6721 gctgctggga gagatcccct ggcttgcaga aaatccagaa aatgcggcaa ccggaaagta
     6781 cataaacctt gccttgttgt agccattctg tatttggtta aattgcgagc gagatcgcgt
     6841 cttcgattga ctgcaattta accaattaaa ttctaaaata atcacgaaaa aaattttact
     6901 tccgcctcat gcggcgaatg tgggaattgc ccaggcggcg ggggataggg gctggagaca
     6961 gttatccact attcctgtgg ataaccatgt gtattagagt tagaaaacac gaggcaagcg
     7021 agagaatacg cggcttgcac gcgaattggc gttaaagacg gctcaaagaa atatctttta
     7081 ttttttaact ggttagataa atgcaatggc agtcactgaa caggcatctc ttgccataaa
     7141 actgtcatca ctcatcttga caaatgttaa aaaagccgtt gctttgggga taacccggta
     7201 aggctggagt tttatctcgc cacagagtaa attttgctca tgattgacag cggagtttac
     7261 gctgtatcag aaatattatg gtgatgaact gtttttttat ccagtataat ttgttggcat
     7321 aattaagtac gacgagtaaa attacatacc tgcccgccca actccttcag gtagcgactc
     7381 atgagtaaac cgttcaaact gaattccgct tttaaacctt ctggcgatca gccagaggcg
     7441 attcgacgtc tcgaagaggg gctggaagat ggcctggcgc accagacgtt acttggcgtg
     7501 actggctccg ggaaaacctt caccattgcc aatgtcattg ctgaccttca gcgcccaacc
     7561 atggtacttg cgcccaacaa aacgctggcg gcccagctgt atggcgaaat gaaagagttc
     7621 ttcccggaaa acgcggtgga gtatttcgtc tcctactacg actactatca gccggaagcc
     7681 tatgtaccga gttccgacac cttcattgag aaagatgcct cggttaacga acatattgag
     7741 cagatgcgtt tgtccgccac caaagcgatg ctggagcggc gtgatgtgat tgtagtggcg
     7801 tctgtttccg cgatttatgg tctgggcgat cctgatttat atctcaagat gatgctccat
     7861 ctcacggtcg gtatgattat cgatcagcgc gcgattctgc gccgactggc ggagctgcaa
     7921 tacgctcgta atgatcaagc attccagcgt ggtactttcc gcgttcgtgg cgaggtgata
     7981 gatatcttcc cggcagaatc ggatgacatt gcacttcgcg tggaactgtt tgacgaggaa
     8041 gtggaacgat tgtcgttatt tgacccgctg accgggcaga ttgtttccac tattccacgt
     8101 tttaccatct acccgaaaac gcactacgtc acaccgcgcg agcgcatcgt acaggcgatg
     8161 gaggagatca aagaagaact ggccgccaga cgcaaagtgc tgttggaaaa caacaaactg
     8221 ctggaagagc agcggctgac ccagcgtacc cagtttgatc tggagatgat ggacgagctg
     8281 ggctactgtt cggggattga aaactactcg cgcttcctct ccggtcgtgg accgggtgag
     8341 ccaccgccga cgctgtttga ttacctgcct gccgatgggc tgctggtcgt cgatgaatct
     8401 cacgtcacca ttccacaaat tggcggcatg tatcgcggtg accgggcgcg taaagagaca
     8461 ctggtggagt acggcttccg cctgccatca gcgctggata accgtccgct taagtttgaa
     8521 gagttcgaag cattagcgcc gcaaaccatc tatgtttcgg cgacgccggg taattacgag
     8581 ctggaaaaat ccggcggcga tgtggtggat caggtggtgc gtccaaccgg attgcttgac
     8641 ccgattatcg aagtgcggcc ggtggcgaca caggttgatg atcttctttc ggagattcgt
     8701 cagcgagcgg caattaacga acgcgtgctg gtcaccacac tgaccaagcg aatggcggaa
     8761 gatcttaccg aatatctcga agaacacggt gagcgcgtgc gttatcttca ctcagatatc
     8821 gacaccgtcg agcgtatgga gattatccgc gacttgcgtc tgggtgagtt cgacgtattg
     8881 gtagggatca acttactgcg tgaaggtctg gatatgccgg aagtgtcgct ggtggcgatc
     8941 ctcgacgctg acaaagaagg cttcctgcgt tccgaacgtt cgttgatcca gaccattggt
     9001 cgtgcggcac gtaacgttaa cggtaaagcg attctctacg gcgataggag cactccatca
     9061 atggcgaaag cgattggcga aaccgaacgt cgccgtgaga aacagcagaa gtacaacgag
     9121 gaacacggca ttacgccgca aggcttgaac aagaaagtgg tcgatatcct ggcgctgggg
     9181 cagaacattg ccaaaaccaa agcgaagggc agaggaaaat cgcgcccgat tgttgagccg
     9241 gataatgtgc cgatggatat gttgccgaaa gcgttgcagc agaaaatcca tgaactggaa
     9301 gggctgatga tgcaacacgc gcagaatctg gagttcgaag aagcggcaca aattcgtgac
     9361 cagttgcatc agttgcgtga gttgtttatt gccgcatcgt aacaggatag cgaagaagac
     9421 tgatgacaaa cggaaaacag tctgatgcgc tacgcttatc aggcctacat tttctccgca
     9481 atatattgaa tttgcgcggt ttgtaggccg gataaggcgt tcacgccgca tccggcataa
     9541 acaacgcgca ctttgtcttc aataggaagc cggaattttc cttcggattt ccgttaacct
     9601 aaagcctgta acgccttttc cagcgcgtta tgtaacaact ggcggtcatg acgatacgga
     9661 atatcgctgg cctccagtac ctcctggatc acaatccgct ctttcaccgc cgagacatcc
     9721 acttttggcc cgacgatgac cgcatcaatg acctttttac caacatactg ctccataatt
     9781 gccagcttgc tttccagctt caaattagcc gcaggtaaac tcaactcacg ccccagattg
     9841 ccgatataaa ccatcggcgc tggcgtgcgg cgtaatgcct gggcgatttc cttcagcagc
     9901 agaattggca tcaggctggt ataaaaactg ccaggcccaa taatgatgag atccgcttca
     9961 ttgatagcgt gaaccgcctc acgcgttgcg ggtacattag gcgttaacaa taactcttga
    10021 atcggcgtag ttaactggtc gatattgacc tcgccgtaaa cttcatgccc ctgatcgtca
    10081 atcgccatca gatcaacagg atgctctgac attggaatca aatgcgtatc cactttcagc
    10141 agattacgaa ttaaattgat ggcttccaga ggccgcacgc taaggtgatc cagcgccttt
    10201 aacatcaagt ttccgagatt atgaccggaa agttcgccat tgccgccaaa acggtattca
    10261 aacatcgcgg aggcgacgtt cggttccgtt atcagctggt tgaggcagtt gcgcatatcg
    10321 ccccaagcaa tgccgccttc tgaacggcga atacgccccg tcgagccacc attatccgtg
    10381 gtggtgacga tacccgttaa acgagaaccc aaagacgaaa gtgatgagag aacgcgtccc
    10441 agtccatgcc ctccgccgag agcaacgaca cgatcaagat cagccagcgt acgattgcgc
    10501 atatagtttc ctggagtcag attatccgcg ctacagtagc gcaaattgcg gggaaacagc
    10561 aattaacctg ccaatattga ggatacaaac tattttcttc tatctctaaa ggacgatgca
    10621 cgctatgcct ccctgatgat gtatatcaaa gtaaaaccgc cattttccct tattctgtag
    10681 cgaaatagca cgatcatgac gctatataca tgattacata gcgaaagtgt ggatggtaaa
    10741 aatctcattt acacgctagt atcggcttaa ccactaaaca ctctagcctc tgcacctggg
    10801 tcaactgata cggtgctttg gccgtgacaa tgctcgtaaa gattgccacc agggcgaagg
    10861 aagaaatgac ttcgcctccc gtatctggaa aggtgtacat ggcttcacaa ctgactgatg
    10921 tatttgcgcg taagttttac tacttgcgcc tgtcgattac cgatgtgtgt aactttcgtt
    10981 gcacctactg cctgccggat ggctacaaac cgagcggcgt caccaataaa ggctttctta
    11041 ccgtcgatga aattcgccgg gttacgcgcg ccttcgccag tctgggcacc gaaaaagtgc
    11101 gcctgacagg aggagagccg tctttacgcc gcgactttac cgatatcatc gccgctgtgc
    11161 gggaaaacga cgctatccgc cagattgcgg tcaccaccaa tggttaccgt ctggaacgcg
    11221 atgtggcgaa ctggcgcgat gcgggactta ctggcattaa cgtcagtgtc gacagtttgg
    11281 acgcccgcca gtttcacgct attaccgggc aggataaatt caaccaggtc atggcaggga
    11341 ttgatgctgc atttgaggcc ggttttgaga aggtcaaagt caataccgtg ctgatgcgtg
    11401 atgttaatca tcaccagctc gacacctttc tgaactggat ccagcatcgc cctatccagc
    11461 tgcgtttcat cgaactgatg gaaacgggcg agggcagcga gctcttccgt aagcatcaca
    11521 tctctggtca ggttctgcgt gacgagctac tgcgtcgcgg ctggatccac caattacgtc
    11581 aacgcagcga cggtcccgcg caagtctttt gccatccaga ttacgccgga gagattggcc
    11641 ttatcatgcc gtatgaaaaa gacttctgcg ccacttgcaa ccgcctgcgc gtttcctcca
    11701 ttggtaaact ccatctctgc ctgtttggtg aaggcggcgt taacctgcgc gatctgctgg
    11761 aaggcgatac ccagcaacag gcgctggaag cgcgtatttc agcggcgctg cgggagaaga
    11821 aacagaccca tttcctgcat caaaacaaca ccggtattac gcaaaactta tcgtacattg
    11881 gcggctaaaa cgtcaaaagg agagatcaga tgagtcaggt aagcactgaa tttatcccga
    11941 cccgtattgc tattcttacg gtttctaatc gtcgcggtga agaagacgat acctccggtc
    12001 actatctgcg cgattcggcg caagaagcgg gccatcacgt tgtcgatgaa gccattgtga
    12061 aagaaaaccg ctacgctatt cgcgctcagg tatctgcgtg gatcgccagc gacgatgtac
    12121 aagtggtatt gattacgggt ggtactggcc tgacggaagg tgatcaggct cccgaagcgc
    12181 tgctgccgtt gttcgaccgt gaggttgaag gttttggtga agtgttccgt atgttgtcgt
    12241 ttgaagagat tggcacttcc acgttgcaat ctcgtgcggt agcgggcgtc gctaacaaaa
    12301 cgctgatttt cgccatgccg ggttcgacca aagcgtgccg taccgcatgg gaaaatatca
    12361 tcgcgccgca gctggatgcc cgtacgcgtc cgtgtaattt ccatccacat ttgaagaaat
    12421 aagtatgtcg caactgaccc atatcaacgc cgctggcgaa gcgcacatgg tggatgtctc
    12481 cgccaaagcg gaaaccgtgc gtgaagcgcg ggcggaagcc tttgtcacca tgcgcagcga
    12541 gacgctggcg atgattattg atggtcgcca ccacaaaggc gacgtatttg ccactgcgcg
    12601 tattgccggt attcaggcgg caaaacgcac ttgggatctg atcccgctgt gtcacccgct
    12661 gatgctcagc aaagttgaag tcaatttaca ggccgagccg gagcacaatc gggtgcgtat
    12721 agaaacctta tgccgcctga ccgggaaaac cggtgtcgaa atggaagcgt taaccgcggc
    12781 ctccgtggcg gcgctgacca tttatgacat gtgcaaagcg gtgcaaaaag atatggtgat
    12841 tggtccggta cgtttgctgg cgaagagcgg cggcaagtcg ggtgacttta aggtggaagc
    12901 ggatgattaa agttcttttt ttcgcccagg tgcgcgagtt ggtgggaaca gatgcaaccg
    12961 aagtggctgc ggattaccca accgttgaag cgttacgcca gcacctggct gcgcagagcg
    13021 atcgctgggc gctggcgctg gaagatggca aattgctggc tgccgtcaac cagacgctgg
    13081 tgagttttga ccatccgctg actgacggcg acgaagtagc tttcttcccg ccggtaaccg
    13141 gaggttaaga tggcagaaac caaaattgtt gtcggcccgc agccgttcag cgtaggagaa
    13201 gagtacccgt ggctggcgga gcgtgacgaa gacggtgcgg tagtcacctt caccggaaag
    13261 gtgcgcaacc ataacctggg cgacagcgtc aaagcattaa ccctcgaaca ctatccgggg
    13321 atgactgaaa aagcactggc agaaattgtt gatgaagcgc gtaaccgctg gccgctgggg
    13381 cgcgtcacgg tgattcaccg catcggggaa ttatggccgg gcgatgaaat cgtttttgtc
    13441 ggtgtcacca gtgcgcatcg cagcagtgcg tttgaagccg ggcagtttat tatggattat
    13501 ctcaaaaccc gcgcaccgtt ctggaagcgc gaagccacgc cggaaggcga ccgctgggtt
    13561 gaagcccggg agagcgatct gcaggcggca aaacgctggt agttttttgt tagccggata
    13621 aggcaccgct gcgtccggca atttcaccgc tcagtacatg cgtttacgct gcgtcgatgc
    13681 acagcctcat cattttgcag tatccttaag atattcctta tatcttcagg agatcgtcat
    13741 ggacagattc ccacgttctg attcaatcgt acaaccctgg gctggcttgc aaacttatat
    13801 ggcgcaagtc tatggctgga tgaccgttgg cttgttgctg accgcatttg ttgcctggta
    13861 tgcggctaat tccgcggccg tgatggagct gttgttcact aaccgtgtct ttttaatcgg
    13921 tctgatcatc gcgcaattag cattggttat tgtgttatca gcgatgattc aaaagctgag
    13981 cgcaggtgta acgacgatgc tctttatgct ttattcggcg ctgacgggtc ttacgctttc
    14041 cagtatattc attgtctata ccgctgcttc tatcgccagt actttcgtcg ttactgccgg
    14101 gatgttcggc gcaatgagcc tgtacggtta caccacgaag cgcgatttaa gtggctttgg
    14161 caatatgctg tttatggcgt taatcggcat tgtgctggca tcgctggtca acttctggct
    14221 gaaaagcgaa gcattgatgt gggcagttac ctacatcggc gtgattgtct ttgtcggatt
    14281 gacggcgtat gacactcaga aattgaaaaa tatgggtgag cagattgata cccgcgacac
    14341 gtcgaacctg cgcaaatatt ccattcttgg tgcgttaacc ttgtatctgg acttcatcaa
    14401 cctgttcctg atgttgttac ggatcttcgg caaccgccgt taattctttt tcaccatcac
    14461 tatcccggcc atgtgtcggg atttttttct tactaaaaac gacacttaac aagtaccagg
    14521 tataaaatat gccttatgag cgagcggaat cgttttccgt atccataaat acaaaagtat
    14581 tttgtatgcg tggttttaaa tttattgatg gtgaatatta atgttttttc taaggacaac
    14641 taaaagaatg gaatcatact cgcaaaacag caataaatta gattttcagc acgaggccag
    14701 gatattaaac ggtatatggc tcattaccgc tttaggtttg gtggcaaccg caggactagc
    14761 ctggggagct aagtatctcg aaattacggc aaccaaatat gattcaccac caatgtatgt
    14821 cgccataggg ttattattac tttgtatgta tggcttaagt aaggatatca acaagataaa
    14881 tgccgccatc gcgggcgtaa tatatctgtt tttactctct ttggtggcga ttgtcgttgc
    14941 aagtttagtt cctgtatatg ccattatcat cgtgttcagc actgcgggcg cgatgttttt
    15001 aatcagtatg ctggccggtt tattatttaa tgttgatcct ggttctcacc gttttatcat
    15061 tatgatgacg ttgacagggt tggccctggt aatcatcgtg aatgcggcat taatgagtga
    15121 acggcccatt tgggtaataa gttgcttaat gattgtgtta tggccaggca ttatctcgca
    15181 tggacgaaat aagctccttg aactggcagg aaaatgccat agtgaagagt tgtggagtcc
    15241 ggttcgttgc gcttttacag gtgcattaac actctattac tattttatcg gcttctttgg
    15301 gatacttgcc gcgatagcta taacgcttgt ctggcaaagg catacgcgtt tttttcatta
    15361 gtcgtaaatg cagatactaa agaacttaac taaacttcac atcgccgctt catttttcgc
    15421 ccgtagcttc ttcgcttggc tttccagtag caggtaacag atcagcgcca gcagcagcgg
    15481 gataaagtaa tacaggacgc gataagcgag cagggcggcg ataatgctcc ctttcgaggt
    15541 atgctcccca gccagtagtg cgataaacac cgcttccagc acaccgatcc ccgccggaat
    15601 atgcacgatg acgccagcaa tactactaac cagtaacacg cccagtacaa agaaatagtt
    15661 cacgctttga ccaagtaaca gccagataat cgcccccatt accatccagt taacgctgga
    15721 aatcagcatc tgagcgaggg cgaatttcca tgaaggcagc accagctttt gccctttgat
    15781 ggtcatatgg cggtgcttcg cgaaagcgca aaaccacaaa taaaccgcga taatcatcag
    15841 taagccaatg ccgagaatac gcagcgtagt ttgatcgaca taccagtaat ccggcaactc
    15901 caccacgcct gcggtaaaga taatccctgc cagtaaaata tagcccagcc agttggtggt
    15961 aatactgagc gaggaaatcc gcgtaatagt gctgcccggt aaccctagtc gggagtacaa
    16021 acgatagcgc ataccaatgc cgccgaccca ggtactgagc gtcaggttga aggcgtagca
    16081 gataaacgac accagcatca cctggcgctt cgccagtttg tgaccgcagt aaaagcgggc
    16141 gagcaggtca tagcagccgt aaatcagata gctgacgacc accagcccga ccgcactaag
    16201 cagcgcaacg cgattgtagt cgcggatgac cttccagacc tcttcccagt ccactttttt
    16261 ggcgtagacc accagcaaca caatcaccgc gataaaaaac agccaggtga ggatcttctt
    16321 tgctaagcgc cagcgcgggt gtgatttact catcaggggt ttacccccgt gttttcagtt
    16381 tctacccgat cctgcgtttc cattgtcggt tgtgcggggg gatcaacctg cgtcagacgt
    16441 ggtgtgtgtg ccggaagcca gccaaccagc gccgggaagt ggcgtaaaaa gtggaacgcc
    16501 agcacgcttt tggtcaggtt ccaccaggtg cgtttgggca gcatggtttc atccacctgc
    16561 tgacaatctg cggcaataat gccgttcaga ttatcgcgca gcgtctggtt aaaatgacga
    16621 tcgtggatga tgacatttgc ttcgagattc agtgacaaac tgagcggatc gagattactg
    16681 gaccctactg tcgcccagtg atcgtccatc aatgccactt tgccgtggag cgggcggcgg
    16741 cggtactcaa aaacctgaac gccgccttta accagatagt tatacagcaa gcgcgcaccg
    16801 actctgacaa tcggcatatc cggttcgccc tgaatgatca gtttgatccg caccccgcgc
    16861 cgtgccgctt tacgcaaggc gtgtaaaaat cgatagccgg ggaagaagta ggcgttggcg
    16921 ataatcactt cccgctgcgc ctgagtgagc attttcagat aatggcgttc aatatcatcg
    16981 cgatgttctt cgttatcgcg ccagaccagc aatacctgcg cttctcccgg ctggcggttc
    17041 tcttccgctt tgtgatgacg tcgccaccag cgtcgtgccg cgctctgtcc aggcaggttt
    17101 tccagctcaa actggagaat atcttcgaca atcggccctt caaggcgtac cgcgtaatcc
    17161 tgtttagcct ctggaccgta gctggacata tgctcggcgg agtaattcag cccgccaata
    17221 aaggcgatac gcgcgtcgat caccacaatt ttgcgatgca tccggcgaaa cacattggtg
    17281 cgcataccaa aaaggcgagg gcggggatcg tagtagcgga acactacgcc agctgccgtc
    17341 agttcattga caaactcatc gctgagatcc ggcgaaccgt agccatccag caagacttcc
    17401 gctttaaccc cgcgttgcgc tgctgccagt agtgccgcat gcagctgttt gccgacgtta
    17461 tcctcaaacc agataaacgt ttcaagaatg atgcgttctt gtgcctcgcc aatcgcctta
    17521 aacaccgcgg gataatattg ctcgccgttt tccagcaact ggatcttatt gccttcgcgc
    17581 cagctacatt tcataaatga atctccgcac ttaaaggggc atgatcagaa aggtgtcgcc
    17641 atgtccgcag cggcaacgcg gttggcgcgc tggcgctggc atttttgacg tagatcctgt
    17701 ccagtcgtag tagaggaaat tgcaccggaa acgtgcgcgc cgggcgtccg tgggcgcggg
    17761 taaaaatctc atccagtccg gcctgcactt ttaacggatg attagctttt tgccgccagt
    17821 cattgaaatc acccgccacc aataccggtt cgccgtccgg tagctcattc acccattcgg
    17881 cgagcatcgc aagctgcgcc tgacggtgcg cctcacgcag gcccagatgt acgcacatca
    17941 catgaatcgc ttttccggtc atcggcggca caatgcggca gtagagcacg ccgcgctttt
    18001 ccgcaccatc gaccgaaaca tcgcgattct cataatgttc aatgggataa cgcgacagta
    18061 cggcgttgcc gtgatgccct tccgggtata cggcattgcg accgtaggca aaatcgctcc
    18121 acatagtgtc ggcgagaaac tcgtagtgcg aggtatcggg ccagttttcc acatgcagcg
    18181 gatgaacttc gtgcgcgccc atcacttcct gcaggcaaac aatatcggcg ctgacggtac
    18241 gcacggcatc gcgaagttcc ggcaaaatga agcgtcggtt aaacgcggta aagcctttgt
    18301 gaatgttgat ggtgagcacc ttgaacgaaa attgttgtgt ttgatcgggc ataattttcc
    18361 tgtctttgcc tcttatctca ttgaaatagt gtagtcggcg tcacaaaaag gtgcggtctt
    18421 acggaatttt ccgtaaagtt cggtactctg agtaagtaga gataaattct tcaggagaga
    18481 agccatgaag tggcaacaac gtgttcgtgt cgcaacgggt ctaagttgct ggcagattat
    18541 gttgcattta ctggtagtgg cgctgctggt ggtgggctgg atgagtaaga ctctggttca
    18601 cgtcggcgtg ggattatgcg cactgtattg tgtcacggta gtgatgatgc tggtgtttca
    18661 gcgccacccc gagcaacgct ggcgtgaggt ggcagacgtg ctggaagagc tgaccacgac
    18721 ctggtatttt ggcgcagcgc tgattgtgct gtggctgttg tcccgcgtac tggaaaacaa
    18781 ctttttgctg gcaattgcag ggctggcgat ccttgctggc ccggcggtag tgtctttgct
    18841 ggcgaaagat aagaagttac atcaccttac gtctaaacat cgcgtacgcc gctgaccctg
    18901 tcgtggccgt tatcaccagt agcggccaca aactattcca cacaatatcc agactcgcat
    18961 ccttcaaata aatctgcttg gtaatgtccg taaagtggcg aatagggtta atccacgtca
    19021 gattttgcag ccacactggc atgttttcca ccggcgaaac gtaaccggaa aggagaatgg
    19081 cgggcatcat aaaaacaaac acgccgataa acgcctgctg ttgtgtcgag cagagtgatg
    19141 aaatcaacag accgaatccc accagcgata aaccgtaaat caccatcgta aagtagaaca
    19201 gcgctagcga tccggcgaag gggatttgat acgcccagat accaatcgcc agcacaatgg
    19261 tggcctggaa ggtggcgaca attaacgccg gtacggcttt gccgatgaag atctgccagg
    19321 tggtgagcgg cgaaaccagt agctgatcga gcgtaccttg ttcacgttcg cgggcgacgg
    19381 aaagtgaagt gacgatcatt acgccgatag tggtgatcat ggcgatcagc gacggcacca
    19441 caaaccattt gtagtcgaga ttcgggttat accagttgcg taccaccagc tcgctgttgt
    19501 tgggtttcgg ttttccttcc agcagctcct gctgataatt tttgacgatc tgttgcaggt
    19561 agttggcggc aatttgcgca ctgttggagt tacgcccgtc gaggatcaac tgcaacggcg
    19621 cggtctggaa ggtatccagt ttgcgcgaga agtcagccgg gaaacgcacc agtagtaacg
    19681 ccttttgtgt gtcgatggtc gggcggatct cctgtgggct tttcagcagc agcacatgtg
    19741 taaaggcgct ggcgcgggca aaacgttgag taagttccac cgaatgctcg ccgttatctt
    19801 catcgtagat ggcgatggtg gcgttagtca cttccagcgt ggcggcgaac gggaacagga
    19861 tcacctgaat tagcacgggt aaaatcagaa tcgcgcgggt ttgtggttcg cgcagcaacg
    19921 actgcaactc tttgcggatt aacgtccata agcgatgaaa catgctcttc tccctaatcc
    19981 agccgacgtt tggttttcag ccacgtcagg ccgataaaca tcaccgccga agcgatcaaa
    20041 aacagcacgt ttaccaccag caccactgga atattcccgg cgaggaacag gctttgcagg
    20101 gtgctgacga aataacgagc gggaataatg tacgtcaccg cgcggatcac cgcgggcata
    20161 ctgtcgatct gaaaaataaa gccggaaagc ataatcgacg gcagaaaagc ggcgttcagg
    20221 gcgacctgag cggcattgaa ctggttgcgg gtaatcgtgg aaatcagcag ccccattccc
    20281 agggtactga gtaaaaacag gctggagata aaaaacagaa tcagcagcga accgcgatac
    20341 ggcacgccga gaataaacac tgacaccagc atacacagca acatcgccag catcccgaga
    20401 aagtaataag ggatcagctt acacagcagc agttccgtgc gggtaatctc cgtagagagc
    20461 agagcctcca tggtgccgcg ttcccattct cgcgccacca ccagcgaggt gagaatcgcg
    20521 ccgatgaccg tcatgataat ggtcaccgca cccgggataa taaagtgctg gcttatcgcc
    20581 gccgggttaa accagtagcg ggtttgtaca tcaataagcg gttcaaaagt ctgcccgttg
    20641 tcctccgctc gctgcatttg ccagatctgc cagatccctt cgacataccc ctgcacaaag
    20701 ttagcggtat tcggctcact gccgtcggtg atcacctgaa tcggtgcggt ggcgttggcg
    20761 cgctccatct gttcggcgaa atccacggga ataaccacca gaccgcgaat tttccccgac
    20821 tgcattttgg cgatcagttc ctgacggtta tcgctgatgg tggcgtcgat gtagggcgaa
    20881 ccggtcatgg tgtgggtgaa atccagcgcc gcttcgctac gctgttccag caaaatcccg
    20941 acccgcagct tgctggagtc gaggttaatg ccgtaaccaa aaataaacag cagtagcagt
    21001 gggatcacta ccgcaatcag ccagctactc ggatcgcgaa cgatctgccg cgtctctttg
    21061 acgcacagcg cccgcacgcg atgccaggaa aggatcgggt tactcattgc tatgctcctt
    21121 atcccagtcg tggatcaact gaataaaggc ttgctccatg gtgggatcgg gttgctcatc
    21181 gttagctgac tgtgctttca aatcgtccgg cgtaccgctg gcgattaatt tcccacggta
    21241 caccaggccg atgcggtcgc aatattccgc ttcatccata aagtgggtgg tgaccatcac
    21301 cgtgacgcct ttctctacca tgctgttgat gtgcagccaa aattcacggc gggtgagggg
    21361 gtcaacgccg gaagtcggtt cgtcgagaaa gagaatatcc ggttcatgca tcagcgaaca
    21421 ggccagcgcc agccgctgtt taaaacctaa tggcagttca tcggtggcgt gtgaggcaat
    21481 acttttcagg ccaaatgcct cgctcatacg ggagattttt tcgttctgcg cccgaccgcg
    21541 taagccatac acaccagaga aaaagcgtaa attctgttcg accgtcaggt tgccgtagag
    21601 cgaaaatttt tgcgccatat agccgagatg ctggcgcgct ttaccggaac tctctttcag
    21661 atccatcccc agcaccagcg cctggccgga agtcggcacc agcaaaccgc acatcatctt
    21721 aaaggtggtc gatttacccg cgccgtttgg ccccagcaaa ccaaaaatct ccccacgttt
    21781 aacggcaaag ttgacgtgat cggtggcggc aaaatcacca aatttcttgg tcagttcttt
    21841 cgcttcgatc accgtctcgc cgggtgtgcc ttctaccgta tgcaatattg cacccagcgg
    21901 cgattctgag gttccggcac cgcccagcaa atcaataaac gcatcttcaa aacgcggcgt
    21961 agtttcgttg atgttgattt ccggcatccc gtcggcatgg cgaatatcgt ctggtgtggc
    22021 ctcttttttg aggatcagac gtaccgattt cccctgaatc atgccatcgc tgacctgcgg
    22081 cagtttcaag gcgcgttgca acagtttgcg gttgccctcg tgtggactgg tcatcagaaa
    22141 gctgcgtccg gccatggttt gtgtcagggc ttttggttct ccctgataca gcaactcgcc
    22201 ttcgttcatc agtaacacgt cacggcactg ctcggcttcg tcgagatacg aggtactcca
    22261 gaggattaac atcccttcgc ccgccagctc atgcaccatc tgccacagtt cgcgccgtga
    22321 gatagggtca acgccgacgc cgggttcatc gagcagcaac actttcggtt cgcccaccag
    22381 ggtacaggcc aggccgagtt tttgtttcat cccaccggag agcttgcccg ccaggcgtcc
    22441 ggtaaacggc ccaagagacg taaactccag caggcgagca aaagtttgct tacgtgcctc
    22501 gccggtgacg ctgcgcaaat ccgcgtacag attgaggttc tccatcaccg tgagatcttc
    22561 atacagacca aatttctgcg gcatatagcc gagcacggcg tgcagcgcgc cgtcgttttt
    22621 gatcggatca aagccaatca ccgtggcact gccgctgtcg ggtttcagta atcccgccaa
    22681 catccgcatc agtgtggttt tacctgcacc gtccggcccc accaaccccg tcacataacc
    22741 ggcgtgaatg gtacaatcga gcggcgcgac ggcgggcttg tccatgcccg gaaagcgttt
    22801 ttccaggccg ttcagcgtga taacggcatc attcatgtcc tgcctcgtca ccgaattgta
    22861 ccgtcactgg cattccctgg cgtaacgcat catcggcgtc ggtcaccaca atacgcaggc
    22921 gatagacgag gtcggtacgc agatccggtg tttcgacggt tttcggggta aattcagcag
    22981 tcggcgaaac gaaaccaatc tgcccgtgat acggcttgtc cgggcgacca tcggtataaa
    23041 gcagcacttt gcgccccggc tgggcctggt caagattccg ttcatcaaca taagcgcgca
    23101 cccacaccgg acgcgttagt gaaacggtaa acaccgtgcc accttcattg agaaccgtgc
    23161 ctggctctac cgcgcgcgtt aacagcgtgc catcagacgg tgctatcaac gttgagtcct
    23221 gtaaattcag ctccgcctgc gccagttgcg cctgcgcctg ttcgaggctg gctttcgcct
    23281 gagcgatgtc ctgttcacgg ttaccggaac ggtactgacg caatttatcc tgtgctgatt
    23341 tcagcgttgc ctgtgcctgg tcgcgcgagg agcgggcatt ttccaggtca tttgccgaaa
    23401 tggtgcggct tttccacagt ccttgctggc ggttatagaa gttctgcgca tagtcatagg
    23461 cggcttgcgc ctgtttcacc gctgcggcgg cctgagcgat ttcttcatcg cgatacccgg
    23521 caagcatcag atcatactgc gcctgtgcca ccgagacgcc cgctttcgcc tgcatcaggg
    23581 caatctcata cggcttgtga tccagttcgc ccagcacctg gcccgctttg atagcatcac
    23641 cttcgtccac cgccagcgat tcaacgcgcc ccccaacacg gaaactaaga ttcaccgtac
    23701 gaatatccac gttgccatac agcgtcaggc cgttatcctg gcggctttga taccaccagt
    23761 agcctccggc aaccacggcg gcaagtacca ctaccgccag tccgatcacg acaggttttt
    23821 tcatcactac aaactccttt gcgataatcc ttgcaaaatc aggtcgatat gacaggtcac
    23881 cgtctggttg atcagttcgg tcttttcttc atcgaacgcg gtccagccgg tacgtaacag
    23941 aattgtttct ttgccaagac ggaacgccag aatctcacca atcaacgcat gggtatgaag
    24001 gatcatgcgg gtgtcattag cgtcgcagcc ggtccaggcg gcaatcaggc gtgtcaggtg
    24061 gctgtgtagc ggactaatca cctgttcgtg caccaggtgg taggctgccg tgggagagag
    24121 ctgctcacgg gagataaact tgctgaggtt gacggtgtca tcctgggtga gcagcttaat
    24181 catgttcctg caggcgcgaa ggatcagttc acggatggca gcccgatcag gctgtggttg
    24241 ggcgaacaag cgttcggctt cctcggcatg cggacggaac tgctcgccaa taaaatcggc
    24301 aatccactgg gcgcaggcga ggtacaaatc ttctttcgaa ccgaagtagt aggtgatggc
    24361 agcgatattc tgcccggcct gggcggctat ctcgcgggtg gtggcgttca ttccatattc
    24421 accaaactgc gccagtgcgg cagcaatcag ctgttttttc gcctgttcac ccttgattgt
    24481 catggcagga ttattcatcg cacagtccat tcttaatcaa atgattgatt aagattatga
    24541 ctccatatgg gagttgtcca gtatggctaa gaattttagc tacgccagtc acagggataa
    24601 tttatgcgct gcgtcacaaa aactgctaca ctccgctccc tcatgacatt gtggtttttg
    24661 tcattttcct tttcagtatc tccctgaaaa ctacaccggt aacggtcggg gcggttcgga
    24721 gtagttatgt ctttcgattc tttgggttta agccctgata tcctgcgcgc cgttgccgag
    24781 cagggttacc gtgaacccac ccctattcag cagcaggcga tccctgctgt gctggaaggc
    24841 cgcgacctga tggctagcgc ccagaccggc accggcaaaa cagcgggctt tacgctgccg
    24901 ctgttgcaac acctgatcac tcgccagccg cacgccaaag ggcgtcgtcc ggtacgtgcg
    24961 ctcattctta ccccgacccg tgaactggcg gcacagattg gcgaaaacgt ccgtgattac
    25021 agcaaatacc tgaacattcg ttcgctggtg gtgtttggcg gtgtcagcat taacccacaa
    25081 atgatgaagt tgcgcggcgg cgttgatgtg ctggtggcaa cgccgggacg tttgctggac
    25141 ctggaacatc aaaatgcagt gaagctggat caggttgaaa tcctcgtcct cgatgaagct
    25201 gaccgcatgc tcgacatggg ctttatccac gatatccgtc gcgtgttaac aaaactgcca
    25261 gcgaaacgcc agaacctgct gttctctgcg accttctctg acgatattaa agccctggcg
    25321 gaaaaactgt tgcacaaccc gctggaaatc gaagtggcac gccgcaatac cgcgtctgat
    25381 caggtgaccc agcacgttca ctttgtcgat aagaaacgca aacgcgaatt gctgtcgcac
    25441 atgattggta aagggaactg gcagcaggtg ctggtgttta cccgtaccaa acacggcgct
    25501 aaccatctgg ctgagcagct caataaagat ggcatccgta gtgtggcgat ccacgggaat
    25561 aaatcgcaag gtgcgcgtac ccgtgcgctg gctgatttta aatcgggtga cattcgcgta
    25621 ctggtggcga ccgatatcgc tgcgcgcggt ctggatatcg aagagttgcc gcacgtcgtc
    25681 aactatgaac tgccaaacgt accggaagat tatgtccacc gtatcgggcg taccggtcgt
    25741 gcggctgcta ccggtgaagc gttgtcgctg gtgtgtgttg atgaacacaa actgctgcgt
    25801 gatatcgaaa aactgctgaa aaaagagatc ccgcgcattg cgattccggg ctatgagccg
    25861 gacccgtcaa tcaaagccga accgatccag aacggtcgcc agcaacgtgg cggcatcagg
    25921 ctgatgaaca aacgcaaaac tgcctgatgc gctacgctta tcaggcctac gtgaactctg
    25981 caatatattg aatttgaatg cttttgtagg ccggataagg cgtttacgcc gcatccggca
    26041 tttcacaaca agcacttgtc agcaatttga gaacacggga aaaaatttat ctgttttacc
    26101 gcccataagc caccgagacg ttacttctct atagccaatt gttcgcgcaa ctccattaaa
    26161 aggtagccca gtcgattctt gcccttacca ttaccaccgt ctccccagta agcatcgttt
    26221 tccgtatgct caaccagttt tgcgggcgcg gttgccagca agagcacacg cagttctgca
    26281 tgctgttcga atttagcgcg aagtgcttta cgcatcacct gttctttaac cgactcccag
    26341 tttttacgaa gtggcttgga acgattacgc cccatgcgag cggcgaccat gggggaagaa
    26401 acccgacgga tctcttcgcg gtatttttca tcaaggaatt tttgtgcctg aaaatagtgt
    26461 tcggaggtgg gccaggtatt cccgtcaacc ttgatgggcc tggcggcaaa gttagaaaaa
    26521 tcaccgtagt catcgctggt gctgtagaaa tttatgatgg tgtcttgcat gacgtgttgg
    26581 atcctttgtg ctcgaacggg cattaaaccg cattatgttg gtggttattg cgagccgctt
    26641 tccagaaaca gaaaaaccat tacccctgaa aaccgaaaaa tgccacaata ttggctgttt
    26701 atacagtatt tcaggttttc tcatggcatt aaccgccgcg cttaaagcgc aaattgccgc
    26761 ctggtataag gcgcttcagg aacagatccc cgactttatt ccccgtgcgc cgcagcggca
    26821 gatgattgcg gacgtcgcca aaacgctggc cggagaagaa gggcggcatc tggcgattga
    26881 agcccccacc ggcgttggga aaacgctctc ctatttgatc cccggcatcg ccattgcccg
    26941 cgaagagcaa aaaacgctgg tggtgagtac cgccaacgtg gcgttgcagg atcagattta
    27001 cagcaaagat ttaccgctgc tgaaaaagat cattcccgat cttaaattca ctgccgcttt
    27061 cgggcgtggg cgctacgttt gtccacgtaa tctgacggcg ctcgccagca ctgaacccac
    27121 gcaacaggat ctgctggcgt ttcttgacga cgaactgacg ccaaataatc aggaagagca
    27181 aaaacgttgt gcgaagctga agggcgatct cgacacttat aaatgggatg gtctgcgcga
    27241 tcatacggat atcgctatag atgatgatct ctggcgtcgt ttgagtaccg acaaagccag
    27301 ctgcctcaac cgtaactgtt actactatcg tgaatgcccg ttttttgtta ctcgtcggga
    27361 gattcaggaa gcggaagtgg tggtggcaaa ccatgcgctg gtgatggcgg cgatggaaag
    27421 cgaagccgta ctgcctgacc cgaaaaatct gctgctggtg ctggacgaag gtcatcacct
    27481 gccagatgtg gcccgggatg cgctggagat gagcgctgaa atcaccgtgc cctggtatcg
    27541 actacagctg gacttgttca cgaaactggt cgctacctgc atggagcagt ttcgcccgaa
    27601 gaccatccca ccgctggcga tccctgaacg tttgaatgcg cattgtgaag agttgtatga
    27661 gcttatcgcc tcgttaaaca acattctcaa tctctacatg cctgccgggc aggaggcaga
    27721 gcaccgtttt gcgatgggcg aactgcctga tgaattgctg gagatctgcc agcggctggc
    27781 aaaactcacc gagatgctgc gtggtctggc ggagttattt cttaacgatt taagtgagaa
    27841 aaccggcagc cacgatattg tgcgtctgca tcggttgatt ttacagatga accgcgcgct
    27901 ggggatgttt gaggtgcaaa gcaaactctg gcggctggcg tctctggcgc aatcttccgg
    27961 tgcgccggtg accaaatggg cgacgcggga agagcgcgaa gggcagctac acctctggtt
    28021 tcactgcgtg ggaatacgtg tcagcgatca gctggaaagg ctgctgtggc gcagtattcc
    28081 gcacattatt gtcacctccg caaccttgcg ttcgctgaat agtttttcgc gtttgcagga
    28141 gatgagtggt ctgaaagaga aagcgggcga ccgttttgtg gcgctggatt ccccctttaa
    28201 ccactgcgaa cagggcaaaa ttgttattcc ccggatgcgc tttgagcctt ccatcgacaa
    28261 cgaagagcaa cacattgccg aaatggcggc ctttttccgt gaacaggtgg agagcaaaaa
    28321 atatctcggt atgttggtac tgtttgccag cggacgggcg atgcagcgct ttctcgacta
    28381 tgtgacggat ttacgcctga tgttgctggt gcagggcgat cagccgcgtt atcgcttagt
    28441 tgaactgcac cgcaaacgcg ttgccaacgg tgagcgtagc gtgctggtgg gcttacagtc
    28501 atttgccgaa gggcttgatt tgaaaggtga tctgctcagc caggtgcata tccacaaaat
    28561 cgcttttccg cccatcgaca gcccggtggt gatcaccgaa ggggaatggc tgaaaagcct
    28621 caaccgctat ccgtttgagg tgcaaagcct gccgagcgcc tcgtttaacc tgattcagca
    28681 ggttgggcga ctgattcgaa gccacggttg ctggggcgaa gtggttatct acgacaaacg
    28741 cttgctgaca aaaaattatg gcaagcgact actggatgca ttaccggtat ttccgataga
    28801 gcaaccggaa gtccctgaag gtatagttaa aaagaaagaa aaaacgaaat ccccacgccg
    28861 tcggcggcgt taatgatgtg agtcaggtaa ggagtcgtaa atggactatc gcaaaatcat
    28921 taaagagatc gggcgcggga aaaaccacgc gcgcgattta gaccgggata ctgcgcgcgg
    28981 tctgtatgct catatgctca atggtgaagt ccctgacctt gagttgggcg gcgtattgat
    29041 tgcgctgcgt atcaaagggg aaggggaagc agagatgctc ggcttttacg aagccatgca
    29101 aaatcacacc atcaagctga cgccgcctgc aggcaagccg atgccgattg tcatccccag
    29161 ttacaacggc gcgcgtaaac aggctaacct gacgccatta ttggcgattc tgctgcataa
    29221 actcggtttt cctgtggtgg ttcacggagt tagcgaagat ccaactcgcg tgctgacaga
    29281 aaccattttc gaactgatgg gtattacacc aacgttgcac ggcgggcagg cgcaggcgaa
    29341 gctcgacgaa catcaaccgg tgtttatgcc tgtcggcgcg ttttgcccgc cgctggaaaa
    29401 acaactggcg atgcgctggc ggatgggcgt gcgtaacagt gcacataccc tggcgaaact
    29461 ggcgacgcca tttgccgaag gtgaggcact gtgtctttcc agcgtttcgc atccggaata
    29521 cattggacgc gtcgcgaagt tctttagcga tatcggcggg tgggcgttac taatgcatgg
    29581 tactgaaggc gaagtgtatg ctaatccgca gcgctgcccg cagatcaatc tcattgaccg
    29641 tgaagggatg cgggtgctgt atgaaaaaca ggaccctgct ggtagcgagt tactgccaca
    29701 agcaaaagat ccggaaacca cggcgcagtg gactgagcgt tgcctggctg gcagcgaagc
    29761 gattcccgaa tcgctgaaaa tccagatggc ttgctgcctg gtggctacgg gtgaagcggc
    29821 aactatcagc gatggcctgg cgcgcgttaa tcaggcattt taatttttat ctcagccgga
    29881 tgagccacgt catccggctt ttaccccacc gcaatatgaa attcctgcat ctttattgac
    29941 cttcccgcgc ccggcgtgca gcataaaaaa acaacaaaca cataacataa acaggagtta
    30001 accatggaaa gtggtcatcg ctttgatgct cagacgctgc acagttttat tcaggctgta
    30061 tttcgtcaga tgggtagcga ggaacaagaa gcgaaattag ttgccgatca tttaatcgcg
    30121 gcaaacctgg cagggcatga ttctcatggt attggcatga tcccaagcta tgtgcgttcc
    30181 tggagtcagg ggcatctgca aattaaccat catgccaaaa tcgttaaaga ggcgggggcg
    30241 gcagtcacgc tcgatggcga tcgcgcattc ggtcaggtcg cggcacacga agcgatggcg
    30301 ctggggattg agaaagcgca tcagcacggt attgctgccg tggcgctaca taactcgcat
    30361 catatcggcc gtatcggtta ctgggcggag cagtgttcag cggcggggtt tgtctctatc
    30421 cactttgtta gcgtggtcgg tattccaatg gtcgcgccgt tccacggtcg cgacagccgc
    30481 tttggcacca atcccttctg tgtggttttc cctcgtaaag ataatttccc gctgttgctt
    30541 gattacgcca ccagcgccat tgcatttggc aaaacccgcg tcgcctggca taaaggcgtc
    30601 cccgtgccgc caggttgcct gattgacgtt aacggcgtgc cgacgaccaa tccggcggta
    30661 atgcaggagt cgccgttggg gtcgctgttg acctttgccg aacataaagg ctacgccctt
    30721 gcagcgatgt gtgaaattct tggcggggcg ctttctggcg gtaaaacgac gcatcaggaa
    30781 acgttgcaaa ccagccccga tgccattctt aactgcatga ccactatcat catcaacccg
    30841 gaactgttcg gcgcgccgga ttgtaacgcg cagaccgaag cctttgccga gtgggtgaaa
    30901 gcctcgccgc atgatgacga taagccgatt ttgctaccgg gcgagtggga agtgaacacg
    30961 cgtcgcgaac ggcaggagca ggggattcca ttggatgcgg gaagctggca ggccatttgt
    31021 gatgcggcgc ggcagattgg tatgtcggaa gagacgttgc aggctttctg tcagcagtta
    31081 gccagctaaa aaaaagcccg tccagtggcg gacgggcaaa caagggtaac ataggatcaa
    31141 tgagggttag agcatatgcg tctgtcggca aacagacagg gaaatacttg tgctggacgt
    31201 agcgtaaacg cctgattcgt ccaacggttc ggattttgta ggcctgataa gacgcggtag
    31261 cgtcgcatca ggcatctgtt gccggatgcg gcataaacgc cttatccgtc ctacgaatta
    31321 cttataaatt accgcagtac cgcttaattt attgttgttg gtggcggaag tgatgctgta
    31381 tccactggca ccggctgccg ccgctttctc agccagtttc gcttccaggg catcgagggg
    31441 ggatgcgcca tcggcagaaa ccacgccgat tttattcatg ttctgtgcct gggatgccgt
    31501 taccggttcc gccgcgaaca cgccaaatga cagagttgaa agagccatag cagcaacaac
    31561 agtattgata gttttcataa ttaatctctc gcaggtgatt tttgtataag tgacgttgtt
    31621 tcgtcgatgt gatgagtatc acgttttttt acgagagata aaatcgaaga gaattgaccg
    31681 ccttgttcaa ataaattgat tgatatctaa catactgata aaccatttat ttgttgtact
    31741 tatggacttg ttttatgccg cgtggcagtc acagcagcgt taaaggcacg gaatgccacg
    31801 caaaatacaa attatactcg ctatgcatct cgccagttgt agttaacgta actgtctgtc
    31861 tttcgaacct ctgcgattat atcctgtata cgctggtttt tgtaaatctt tctcctgctg
    31921 acaatgaata cataagcgca cgccaggaat ggcttcccga cgggcttgcg ggatgggggc
    31981 accgcactct tcacattcat acaggctttc gccacgcgga atttcacccc gagcgcgggc
    32041 aatcgcatct tcaattgtac tgttgatctg ttcgttgacg gcgtcatcgt tagcccaagc
    32101 ggatgccata tcgtcctccc catatcaatg cttgtacagt taagtgtagc taatataggg
    32161 acgaacctgg gcagttcaag cgtcagatct ccgaccattc ccgcagcaga ttatgataaa
    32221 gattgagcag cgacaggatc tcttcatttt caccatagcg gcttttcagc gactgaatat
    32281 ttttgtccag ttcaaacagc atggcgcgct ttttatcatc gcggatcatc gactggatcc
    32341 acataaatga tgccactcgt acgccgcggg ttacgggtgt cacgcaatgc aggctgctgg
    32401 agggatacaa caccagatca cctgccggga gttttacccg atgttgtcca aaggtgtcat
    32461 taacgaccag ttcgccgccg tcgtagcttt gtggatcgct taaaaacagc gtggcagaaa
    32521 ggtcagtgcg catccagccg ttttgtggat ggctgcgtac tgcgccatcc acatggaaac
    32581 cataggtttc attgttctga tagcgattaa acagcggcgt ggaaagggta cgcggcaagg
    32641 cagcggcaaa gaacaatgca tgttggttaa ccgcgttcag cacctcattt tgcagtgcgg
    32701 cgtataacgt gctgcgggtg tctacttgct gattgttctt aacttgtgca ccttgtgctc
    32761 cggtggtgac gcgtccatcc acccattcgg cttgttccag ttgttcgcga aaatgggcga
    32821 cgtcctgtgg cgataacacg ccgggaatgt ggtacatcat acaagttctc caaaaagtgg
    32881 ggcctgcgcc ccacatctga atcagaaatg catattggct gtgagcaaga aggttcttgg
    32941 ctcgcccggg tgataacggt agccgctctt gttgattgag gcgacgtaat cggtatcaaa
    33001 caggttgtaa acgtttagct ggaagtcgag attgcgatta acccgatacc ccagtttggc
    33061 atcggcgacc cagtaacctt ctgtaaatgc tggcgttccc accgcgccgt ctgaaccttt
    33121 atgtatactg ccgatatagc gtgcgcccgc gccaacagag atatcatcgg ttgcctgata
    33181 ttggctccat aaggtgaagg cgtgctccgg ggtatacggc agcgatgagg aaccatcctg
    33241 ggcaacatct ttgccgtttt tgatggttgc tttttgctgg gtatagccgc caatcacctg
    33301 ccacgcggga gtgatattcc ctgccacgga tatctcatag ccttcgacgc gtttcttacc
    33361 gtattgcgag taagtcccgt catcattttg ctcaacttca ttttcgatat cagtacggaa
    33421 cagcgcggcg gtgagcaaca ggcgtttatc cagaacctgc catttggtgc caatctcgct
    33481 ggtgttggct ttttgcggtt taaaatcggt gcggttggca ctgttaccac tgccagactg
    33541 cgcaagggcg aagttgttgc cgcccggagg ctgctgggaa acggcatagt taatatagac
    33601 attgccgttt tccgtcaggt ggtacagcgc cccggctttc cagttcacca gattgcccga
    33661 cttggcggtg tcgacggtgg tgactggaga accttttgcc acaccagctg ggcaggtgat
    33721 ggcaccgcgt ccgctgccgc cgcaggcggt ggcactgtca tattcagtat gataattatc
    33781 cagacggatc ccgccgttca gctcaaaatc acgggtgatt tgcagcgtat cgaaggcgta
    33841 aattgcgaag gtatccgtct gaccattggc gtttgcgccg ttgcgcgtca ggccgccggg
    33901 atgaattctg ctgtcaggat gataaatatt taccgccggt aacgtcaccg gattaacgcc
    33961 atagttagtc tgcgtttcac gggtaaattc cacgccggta ctgacatcat gaccgataga
    34021 accggtatag aacgtcgagg tcaggttggt ctggttggtg agaattttat tactcacatc
    34081 tttggtattc gccgtgcgtg actaggtcca gctattcaca tcgctggtgg gctgagtaat
    34141 attcgacgcc ccgcccataa tcgccgtcat caggtaatcc tgctttacgc gcgaccaacg
    34201 ggtagtattg cgaatggtgg tgttatcgtt gatgtcgtgc tcaaaacgca tggtggcggt
    34261 gtcggtggtc gaatcgtcgt aatcggaatc cgtgccgtaa aagttatgag tatcaacttt
    34321 tccggaatga ttcagggccg ccgctcccgc agatggggca gaatagcccg gcaaaccgat
    34381 ggtcggaatg ccgccgtctg gcgtgttgtg ctgagtgaca tgcagataat taagatacaa
    34441 acgatttgct gtaccaaggc caaaagcgac agaaggggcg acgccgtaac gctcattttt
    34501 gactttgtcg cgtccggcat cgtgggtttt ttcgcccatc acattcaggc gcaccgcggt
    34561 agtatcacca atgacctgat tgacgtccag cgtgccgcgg cggaaccagg cgctgccaat
    34621 actggcggag gcgtcaatgc cggaatcatt gcgcggctgc ttgctgatca tattgatcga
    34681 gcctgtcggt gcgctgcgcc cgtagtcggt gccggacggc cctttaatca cttcgacctg
    34741 ctcggtattg aaggtgtcgc gcgagacgct gccgatatcg cgaataccat caatataaat
    34801 actgttagag gtatcggcac cacgcatata aatggcgtcg ccagtggtgg agttaccgtt
    34861 ctcacccgca aaaaacgcac ccacgccgg
//
LOCUS       DAJTQQ010000035        34653 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000035 DAJTQQ010000000
VERSION     DAJTQQ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.035"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            695..2980
                     /gene="nrdA"
                     /locus_tag="OO309_001879"
     CDS             695..2980
                     /gene="nrdA"
                     /locus_tag="OO309_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR5847036.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3214..4344
                     /gene="nrdB"
                     /locus_tag="OO309_001880"
     CDS             3214..4344
                     /gene="nrdB"
                     /locus_tag="OO309_001880"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR5847037.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4344..4598
                     /gene="yfaE"
                     /locus_tag="OO309_001881"
     CDS             4344..4598
                     /gene="yfaE"
                     /locus_tag="OO309_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR5847038.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4652..5302)
                     /gene="inaA"
                     /locus_tag="OO309_001882"
     CDS             complement(4652..5302)
                     /gene="inaA"
                     /locus_tag="OO309_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR5847039.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5481..5723
                     /locus_tag="OO309_001883"
                     /pseudo
     CDS             5481..5723
                     /locus_tag="OO309_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5765..6841)
                     /gene="glpQ"
                     /locus_tag="OO309_001884"
     CDS             complement(5765..6841)
                     /gene="glpQ"
                     /locus_tag="OO309_001884"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5847040.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6846..8204)
                     /gene="glpT"
                     /locus_tag="OO309_001885"
     CDS             complement(6846..8204)
                     /gene="glpT"
                     /locus_tag="OO309_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR5847041.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8477..10105
                     /gene="glpA"
                     /locus_tag="OO309_001886"
     CDS             8477..10105
                     /gene="glpA"
                     /locus_tag="OO309_001886"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR5847042.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            10095..11354
                     /gene="glpB"
                     /locus_tag="OO309_001887"
     CDS             10095..11354
                     /gene="glpB"
                     /locus_tag="OO309_001887"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR5847043.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11351..12541
                     /gene="glpC"
                     /locus_tag="OO309_001888"
     CDS             11351..12541
                     /gene="glpC"
                     /locus_tag="OO309_001888"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR5847044.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12734..13684
                     /locus_tag="OO309_001889"
     CDS             12734..13684
                     /locus_tag="OO309_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR5847045.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13725..14195)
                     /locus_tag="OO309_001890"
     CDS             complement(13725..14195)
                     /locus_tag="OO309_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR5847046.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14250..15452)
                     /locus_tag="OO309_001891"
     CDS             complement(14250..15452)
                     /locus_tag="OO309_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR5847047.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15539..16081)
                     /locus_tag="OO309_001892"
     CDS             complement(15539..16081)
                     /locus_tag="OO309_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5847048.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16360..16785
                     /gene="nudI"
                     /locus_tag="OO309_001893"
     CDS             16360..16785
                     /gene="nudI"
                     /locus_tag="OO309_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR5847049.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16824..17426)
                     /locus_tag="OO309_001894"
     CDS             complement(16824..17426)
                     /locus_tag="OO309_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR5847050.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17734..18873
                     /gene="arnB"
                     /locus_tag="OO309_001895"
     CDS             17734..18873
                     /gene="arnB"
                     /locus_tag="OO309_001895"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR5847051.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            18877..19845
                     /gene="arnC"
                     /locus_tag="OO309_001896"
     CDS             18877..19845
                     /gene="arnC"
                     /locus_tag="OO309_001896"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR5847052.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19845..21826
                     /gene="arnA"
                     /locus_tag="OO309_001897"
                     /pseudo
     CDS             19845..21826
                     /gene="arnA"
                     /locus_tag="OO309_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            21823..22713
                     /locus_tag="OO309_001898"
     CDS             21823..22713
                     /locus_tag="OO309_001898"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR5847053.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22713..24365
                     /gene="arnT"
                     /locus_tag="OO309_001899"
     CDS             22713..24365
                     /gene="arnT"
                     /locus_tag="OO309_001899"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR5847054.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24362..24697
                     /gene="arnE"
                     /locus_tag="OO309_001900"
     CDS             24362..24697
                     /gene="arnE"
                     /locus_tag="OO309_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR5847055.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24697..25083
                     /gene="arnF"
                     /locus_tag="OO309_001901"
     CDS             24697..25083
                     /gene="arnF"
                     /locus_tag="OO309_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR5847056.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25077..25349)
                     /gene="pmrD"
                     /locus_tag="OO309_001902"
     CDS             complement(25077..25349)
                     /gene="pmrD"
                     /locus_tag="OO309_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR5847057.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25454..26809)
                     /gene="menE"
                     /locus_tag="OO309_001903"
     CDS             complement(25454..26809)
                     /gene="menE"
                     /locus_tag="OO309_001903"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR5847058.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26806..27768)
                     /gene="menC"
                     /locus_tag="OO309_001904"
     CDS             complement(26806..27768)
                     /gene="menC"
                     /locus_tag="OO309_001904"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR5847059.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27768..28625)
                     /gene="menB"
                     /locus_tag="OO309_001905"
     CDS             complement(27768..28625)
                     /gene="menB"
                     /locus_tag="OO309_001905"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR5847060.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28640..29398)
                     /gene="menH"
                     /locus_tag="OO309_001906"
     CDS             complement(28640..29398)
                     /gene="menH"
                     /locus_tag="OO309_001906"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR5847061.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29395..31065)
                     /gene="menD"
                     /locus_tag="OO309_001907"
     CDS             complement(29395..31065)
                     /gene="menD"
                     /locus_tag="OO309_001907"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR5847062.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31154..32449)
                     /gene="menF"
                     /locus_tag="OO309_001908"
     CDS             complement(31154..32449)
                     /gene="menF"
                     /locus_tag="OO309_001908"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR5847063.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLLPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32528..32833)
                     /gene="elaB"
                     /locus_tag="OO309_001909"
     CDS             complement(32528..32833)
                     /gene="elaB"
                     /locus_tag="OO309_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR5847064.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(32888..33349)
                     /locus_tag="OO309_001910"
     CDS             complement(32888..33349)
                     /locus_tag="OO309_001910"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5847065.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33414..34331
                     /gene="rbn"
                     /locus_tag="OO309_001911"
     CDS             33414..34331
                     /gene="rbn"
                     /locus_tag="OO309_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR5847066.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34519..>34653
                     /locus_tag="OO309_001912"
     CDS             34519..>34653
                     /locus_tag="OO309_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCR5847067.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     E"
BASE COUNT         8460 a         9294 c         8588 g         8311 t
ORIGIN      
        1 ttaatctgac catcatttta tataagacgg catatttaaa cgcattatac aatatgggtt
       61 atctttcttt tgtaaattgt ccaacccgat gcaatggcta ttttgttatt atttgatgaa
      121 taatatcagt gcgtcataat tcaagttaat aaccttaagg gataccagtt atatttaaac
      181 taaattaaag taatgaataa ttttcttata atataaggta aattaacaaa atggcttcgc
      241 atttaacaat aaccgaatag aaaacaacca tttcgacatc aacaatcgct tacattcgct
      301 tatatattga ccacaactga tacatcagat tatgtgatga ctcgtgctta gatcaatttt
      361 tgcaatcatt agcaaaaaga ttaataaacc atctatatca atttatccaa cctattctgc
      421 cgttcaagaa atcgccgaac agttattttt aacaaatttt tctcttccca ttgactttcc
      481 cggacacctt gtctgaccta aggtgcgcga aagccacttt ttccttcctg agttatccac
      541 aaagttatgc acttgcaaga gggtcatttt cacactatct tgcagtgaat cccaaacata
      601 ccccctatat atagtgttct aagcagcttc ccttactaca ggtagtctgc atgaaactat
      661 tgcggaaaga attccaaaaa caggtacgac atacatgaat cagaatctgc tggtgacaaa
      721 gcgcgacggt agcacagagc gcatcaatct cgacaaaatc catcgcgttc tggattgggc
      781 ggcagaagga ctgcataacg tttcgatttc ccaggtcgag ctgcgctccc acattcagtt
      841 ttatgacggt atcaagacct ctgacatcca cgaaactatt atcaaggctg ccgcagacct
      901 gatctcccgt gatgcgccgg attatcagta tctcgccgcg cgcctggcga tcttccacct
      961 gcgtaaaaaa gcctacggcc agtttgagcc gcctgcgctg tacgaccacg tggtgaaaat
     1021 ggtcgagatg ggcaaatacg ataatcatct gctggaagac tacacggaag aagagttcaa
     1081 gcagatggac acgtttatcg atcacgaccg tgatatgacc ttctcttatg ctgccgttaa
     1141 gcagctggaa ggcaaatatc tagtacagaa ccgcgtgacc ggcgaaatct acgaaagcgc
     1201 ccagttcctt tatattcttg ttgccgcgtg cctgttctcg aactacccgc gtgaaacgcg
     1261 cctgcaatac gtgaagcgtt tttatgacgc ggtttccaca tttaaaattt cgctgccgac
     1321 gccaatcatg tccggcgtgc gtaccccgac tcgtcagttc agctcctgcg tactgatcga
     1381 gtgcggtgac agcctggatt ccatcaacgc cacctccagc gcgattgtga aatatgtttc
     1441 ccagcgtgcc gggatcggca tcaacgccgg acgtattcgt gcgctgggca gcccgattcg
     1501 cggtggcgaa gcgttccata ccggctgtat cccgttctac aaacatttcc agacagcggt
     1561 gaaatcctgc tctcagggcg gtgtgcgcgg cggtgcggca acgctgttct acccgatgtg
     1621 gcatctggaa gtggaaagcc tgctggtgtt gaaaaacaac cgtggggttg aaggcaaccg
     1681 cgtgcgtcat atggactacg gggtacaaat caacaaactg atgtataccc gcctgctgaa
     1741 aggtgaagat atcaccctgt tcagcccgtc cgacgtaccg gggctgtacg acgcgttctt
     1801 cgccgatcag gaagagtttg aacgcctgta taccaaatat gagaaagacg acagcatccg
     1861 caagcagcgt gtgaaagccg ttgagctgtt ctcgctgatg atgcaggaac gtgcgtctac
     1921 cggtcgtatc tatattcaga acgttgacca ctgtaatacc catagtccgt ttgatccgac
     1981 catcgcgcca gttcgccagt ctaacctgtg cctagaaatt gccctgccga ccaaaccgct
     2041 gaacgacgtc aacgacgaga acggtgaaat cgcgctgtgt acgctgtctg ctttcaacct
     2101 gggcgcaatt aatagcctgg atgaactgga agagctggcg attctggcgg ttcgtgcact
     2161 tgacgcgcta ctggattatc aggattaccc gatcccggcc gccaaacgtg gagcgatggg
     2221 gcgtcgtacg ctgggtattg gtgtgattaa cttcgcttac tatctggcga agcacggtaa
     2281 acgctactcc gacggcagcg ccaacaacct gacgcataaa accttcgaag cgattcagta
     2341 ttacctgctg aaagcgtcta atgagctggc taaagagcaa ggcgcgtgcc cgtggtttaa
     2401 cgaaaccact tacgcgaaag ggatcctgcc gatcgatacc tataagaaag atctggatgc
     2461 catcgctaat gagccgctgc attacgactg ggaagcattg cgtgaatcaa tcaaaacgca
     2521 cggcctgcgt aactccacgc tttctgccct gatgccgtcc gagacctctt cgcagatctc
     2581 taacgccact aacggtattg aaccgccgcg cggttacgtc agcatcaaag cgtcgaaaga
     2641 cggtattttg cgccaggtgg tgccggacta cgagcacctg cacgacgcct atgagctgct
     2701 gtgggaaatg ccgggtaacg atggctacct gcaactggta ggtatcatgc agaaatttat
     2761 cgatcagtcg atctctgcta acaccaacta cgatccgtca cgtttcccgt caggaaaagt
     2821 gccgatgcag cagttgctga aagacctgct caccgcctac aaattcgggg tcaaaacgct
     2881 gtattatcag aacacccgtg acggcgctga agacgcgcag gacgatctgg taccgtcaat
     2941 ccaggacgat ggctgcgaaa gcggcgcatg taagatctga tattgagatg ccggatgcgg
     3001 cgtaaacgcc ttatccggcc tacggctcgg tttgtaggcc tgataagacg cgccagcgtc
     3061 gcatcaggct ccgggtgccg gatgcagcgt gaacgcctta tccggcctac ggctcggatt
     3121 tgtaggcctg ataagacgcg ccagcgtcgc atcaggcaca ggatgcggcg taaaatgcct
     3181 tatccggcat taaactccca acaggacaca ctcatggcat ataccacctt ttcacagacg
     3241 aaaaatgatc agctcaaaga accgatgttc tttggtcagc cggtcaacgt ggctcgctac
     3301 gatcagcaaa aatatgacat cttcgaaaag ctgatcgaaa agcagctctc tttcttctgg
     3361 cgtccggaag aagttgacgt ctcccgcgac cgtatagatt accaggcgct gccggagcac
     3421 gaaaaacaca tctttatcag caacctgaaa tatcagacgc tgctggattc cattcagggt
     3481 cgtagcccga acgtggcgct attgccgctt atttctattc cggaactgga aacctgggtc
     3541 gaaacctggg cgttctcaga aacgattcat tcccgttcct atactcatat cattcgtaat
     3601 atcgttaacg atccgtctgt tgtgtttgac gatatcgtca ccaacgagca gatccagaaa
     3661 cgtgcggaag ggatctccag ctattacgat gaactgatcg aaatgaccag ctactggcac
     3721 cttctgggcg aaggcaccca caccgttaac ggtaaaaccg taaccgttag cctgcgcgag
     3781 ctgaagaaaa aactgtatct ctgcctgatg agcgttaacg cgctggaagc gatccgcttc
     3841 tacgtcagct ttgcttgttc ctttgccttt gcagaacgcg aactgatgga aggtaacgcc
     3901 aagattattc gcctgattgc ccgcgatgaa gccctgcacc tgaccggtac tcagcatatg
     3961 ctgaatctgc tgcgcagcgg cgcggatgat ccggagatgg cggaaattgc cgaagaatgt
     4021 aagcaggagt gctatgacct gtttgtccag gcggcacagc aagagaaaga ctgggcggat
     4081 tatctgttcc gtgacggttc gatgattggt ctgaataaag atattctctg ccagtacgtt
     4141 gaatacatca ccaatatccg catgcaggca gtcggtttgg atctgccgtt ccagacgcgc
     4201 tccaacccga tcccgtggat caacacctgg ctggtgtctg ataacgtgca ggttgctccg
     4261 caggaagtgg aagtcagttc ttatctggtc gggcagattg acgcggaagt ggacaccgac
     4321 gatttgagta acttccagct ctgatggccc gcgttaccct gcgcatcact ggcacacaac
     4381 tgctgtgcca ggatgaacac ccttcccttc tggcggcgct ggaatcccac aatgtggcgg
     4441 ttgagtacca gtgccgcgaa ggttactgcg gctcctgtcg cacccgactg gtcgcaggtc
     4501 aggtggactg gattgccgaa ccgttagcct ttattcagcc gggggaaatt ttgccctgtt
     4561 gttgccgggc aaaaggcgat attgaaatcg agttgtgatc gggtactagc gccagatacg
     4621 acgcttacgc gtattatctg gcctacaacg attacatggc gtagtaatac gctttcacct
     4681 gctcccagtc cgctttaggg atcggctcca gatatttttc cagctggcgg aagtcatgat
     4741 taatcgcttt atcgcgacgc aagcggcgac gacttttctc cagatcaaga aaaccagctt
     4801 ctgccttacc ttctgttttc acatagatat ggcgaacata gcaacagcca tgctgacgat
     4861 taacgctatg cattttctta aacgccagtg ccactgcttt caacatggct tgccgtactt
     4921 cgtcagaata aggcgatact gcatgccggg cataccagtc agcaatgctg atgaaccccg
     4981 ccatatcttc agtcaccagc aacgcgcgcc attcaccctc aattttcacc gcttcaccaa
     5041 aaacgatctt cggcacgatg acaccggccc gttccagttc tttaattacc gcaacctcac
     5101 ggacaatcgt tggtcggccg aacggataac gtactgaatg aaacagatga tgcgtcatgc
     5161 gctttacata cagctttttg ccgttgcgct cgacgcattg caccccgctc ataccattac
     5221 ggcgatagtt aggctcttca acccagtcgc cctctgttgc ccaccagtga ttaaattcgt
     5281 cgtactttgc tgaaactgcc ataccctatc gcctgtcatt tttattaacg acaatgacta
     5341 taggtggtta cctgaggaaa atcttaatga aacgtgtcgt attaatgaat ttcttttgcc
     5401 gctacaggtg tgacaccgct tttgcccttc atttgcagcg catagattcc accagccact
     5461 accactaata gtgcgcccag cagataaaat gtgaatttaa tcagtcggat acagcgatga
     5521 attttattcg acagggatta ggcatcgcct tacaaccaga gttaacgctg aaaagcattg
     5581 caggtgaatt gtgttccgtt cctcacgaac caaccttcta tcgacagatt tcgttgctga
     5641 ctaaagaaaa gccggtagaa ggcagtccac tgtttttact acaaatgtgc atggaacaat
     5701 tagtggcgat tggaaaaatt tgatatgaga caggatggcg catgaacgcc atcctgcagt
     5761 aacattactc tttattaaga aattttactg ccttatcagg gaaatcagta aacagcccat
     5821 ttacacccgc tttgttatac agagcatcat ataactgatt cacatcagga gtgtattcag
     5881 gcagtttatc tgaccgcacg gtataaggat gcactaccag tttattctgc tgggcatctt
     5941 gcaccatgcc agtgagtttg atattacccg gctgcgatgt ctcctcaatc aacatatggt
     6001 aatccggacc aataccatct gcatattccg ccacctgttt catggctccc ggcttaaaca
     6061 tccagtcgta gttgtaatta acccagcttc catccggctg tttctgctgc gtttcattcc
     6121 agtcggtata ggcaatcagc tgtaccagat tgagctccat gcccattttg ggttccagct
     6181 cattcttaat acgcttcagc tcatcagcat caaaacattg caaataaact ttatcgtctt
     6241 taccggtgta accatatttc ttcagcactt ccagcgtttt tgccgcaata tccttccctt
     6301 cctgatgatg gaaccacggc gctttgattt ctggatagat accgatattt ttcccggtag
     6361 agtgatttaa cccctgaaca aattcaatct cttcttcaaa ggtgtgcacc cggaagtcgg
     6421 acttacccat tgggaaacgc cccggataag tctgtacttt tttaccgttt tcaatatcga
     6481 aaccttcggt aaatttcagc gacttaattt catccagcgt gaaatctatc gcgtagtaac
     6541 gaccgtcttt gcgcgcccga tccgggaaac gatcggcaac atcagtaaca cgatcgagat
     6601 aatggtcatg cagaacaacc agatggtcgt ctttggtcat caccaaatcc tgttccagat
     6661 aatccgctcc ctgcgcatac gccatcgctt ttgctggcag cgtatgctcc ggcaaatatc
     6721 cactggcacc gcgatgggcg atgactattt tttcgttgct gtccgccgcc attgcactgc
     6781 ttcccatgac tatagtgctc atcatgatcg ccatgctaag gtttttcagc ttcaatttca
     6841 tgccattagc ctccgttgcg tttttgcagt aattgttcat ggcgacgttt ttcgccaatc
     6901 atcacaacaa tcaacaagat aaccgccaga atgctgccgc caatcattac cataaagccg
     6961 ccatcccagc cgaagaagtc cacggtgtag ccaacaatcg cgctcgccgc caccgaaccg
     7021 cccaggtaac caaacagccc ggtaaagccc gctgccgtac ctgccgcttt tttcggtgcc
     7081 agttccagcg catgcagacc gatcagcatc acaggaccgt agatcaggaa gccgataaca
     7141 atcatacaaa tcatatcgac ggttgggtta cctgccgggt tcatccagta aacgatagtc
     7201 gcgatggtca ccagtgtcat aaagaagacg ccggttgccc cacggttacc acggaagact
     7261 ttgtccgaca tccagccgca cagcagggtg cccggaatac ccgcatactc atagaagaag
     7321 taggcccagg aggatttatc cagcgcgaaa tgcttaacct ctttcagata agtcggtgac
     7381 cagtcgagga tgccgtaacg cagcagataa acgaacacgt tggcgatggc gatgtaccac
     7441 agcagtttgt tcggcagcac gtactgcatg aagatttgct tcgcggtcag ctcctgttcc
     7501 gctttttcgt tatagtcgtc cggataatca tttttgtact cttcgatcgg cggcaagcca
     7561 caggattgcg gggtatcgcg catcatcgca aaggcgaata atgccaccag aatggcgcag
     7621 aaagcaggca tatagagcgc cgcatgccag tcattgaacc aggccatccc cagcaggaac
     7681 agcagcggcg gaataccacc accgacgttg tgcgcacagt tccacactga cacaatgccg
     7741 ccacgttctt tctgcgacca ccagtgcacc atagtacgac cacacggcgg ccaccccatc
     7801 ccctggaacc aaccgcagag gaacaacagt acaaacatca ccgcaatgct cgacgtcgcc
     7861 catggcacaa agcccataaa caacatcact gccgccgcca gaatcaaacc tgcgggcagg
     7921 aaaacgcgcg gattcgagcg atccgatacc gaacccatga tgaatttcga aaatccataa
     7981 gcaatcgaga tccccgaaag ggcaaaacct aaatcaccgc gtgagaatcc ctgctcaacc
     8041 agataaggca tagcaagcgc aaagttctta cgaaccaaat agtaagccgc atagccaaag
     8101 aatatcccca ggaaaatttg ccagcgcaat cgacgataag tcggatcgat ctccgcggca
     8161 ggtaagcgcg ctttgtgtgg cgctggttta aaaatactca acattgatag cctccgtggc
     8221 ccgtggtctt atttatgatt aacagcctga ttcagtgaga gaacctgccg tttcttgagt
     8281 tgccgcgatg ttaagaaaac attcataaat taaatgtgaa ttgccgcaca cattattaaa
     8341 taagatttac aaaatgttca aaatgacgca tgaaatcacg ttttactttc gaatcatgag
     8401 cgaatatgcg cgaaatcaaa caattcatgt ttttactatg gctaaatggt aaaaaacgaa
     8461 cttcagaggg ataacaatga aaactcgcga ctcgcaatca agtgacgtga ttatcattgg
     8521 cggcggcgca acgggagccg ggattgcccg cgactgtgcc ctgcgcgggc tgcgcgtgat
     8581 tttggttgag cgccacgaca tcgcaaccgg tgccaccggg cgtaaccacg gcctgctgca
     8641 cagcggtgcg cgctatgcag taaccgatgc ggaatcggcc cgcgaatgca ttagtgaaaa
     8701 ccagatcctg aaacgcattg cacgtcactg cgttgaacca accaacggcc tgtttatcac
     8761 cctgccggaa gatgacctct cattccaggc cacttttatt cgcgcctgcg aagaagcagg
     8821 gatcagcgca gaagctatag acccgcagca agcgcgcatt atcgaacctg ccgttaaccc
     8881 ggcactgatt ggcgcggtga aagttccgga tggcaccgtt gatccatttc gtctgaccgc
     8941 agcaaacatg ctggatgcca aagaacacgg tgccgttatc cttaccgctc atgaagtcac
     9001 ggggctgatt cgtgaaggcg cgacggtgtg cggtgttcgt gtacgtaacc atctcaccgg
     9061 cgaaactcag gcccttcatg cacctgtcgt ggttaatgcc gctgggatct gggggcaaca
     9121 cattgccgaa tatgccgatc tgcgcattcg catgttcccg gcgaaaggat cgctgctgat
     9181 catggatcac cgcattaacc agcatgtgat caaccgctgc cgtaaacctt ccgacgccga
     9241 tattctggtg cctggcgata ccatttcgct gattggtacc acctctttac gtattgatta
     9301 caacgagatt gacgataatc gagtgacggc agaagaggtt gatattctgc tgcgtgaagg
     9361 ggaaaaactg gcccccgtga tggcgaaaac gcgcattttg cgggcctatt ctggcgtgcg
     9421 cccgctggtt gccagcgatg acgacccgag cggacgtaac gtcagccgtg gcatcgtgct
     9481 gctcgaccat gctgaacgcg atggtctgga cggatttatc accatcaccg gtggcaaact
     9541 gatgacctat cggctgatgg ctgaatgggc taccgacgcg gtatgccgca aactgggcaa
     9601 cacgcgcccc tgtacgactg ccgatctggc actgcctggt tcacaagaac ccgctgaagt
     9661 taccttgcgt aaagtcatct ccctgcctgc cccgctgcgc ggttctgcgg tttatcgtca
     9721 tggcgatcgc acgcctgcct ggctgagcga aggccgtcta caccgtagcc tggtatgtga
     9781 gtgcgaagcg gtaactgcgg gtgaagtgca gtacgcggta gaaaatttaa acgttaatag
     9841 cctgctggat ttacgccgtc gtacccgtgt ggggatgggc acctgccagg gcgaactctg
     9901 cgcctgccgc gctgccggac tgctgcaacg gtttaacgtc acgacgtccg cgcaatctat
     9961 cgagcaactt tccaccttcc ttaacgaacg ctggaaaggc gtgcaaccca tcgcctgggg
    10021 agacgcactg cgcgaaagcg aatttacccg ctgggtttat cagggactgt gtggtctgga
    10081 gaaggagcag aaagatgcgc tttgatactg tcattatggg cggcggcctc gccggattac
    10141 tctgtggcct gcaactgcaa aaacacggcc tgcgctgtgc cattgtcacg cgtggtcaaa
    10201 gcgcgttgca tttctcatcc ggttcgctgg atttgctgag ccatctgcca gatggtcaac
    10261 ccgtggcaga cattcacagt ggactggaat ctttgcgtca gcaggcacct gcacatcctt
    10321 actctcttct cgggccacaa cgcgtgctcg atctcgcttg ccaggcgcag gcattaatcg
    10381 ctgaaagcgg tgcgcaattg cagggcagcg tagaactggc tcaccagcgg attacgccgc
    10441 tcggcacttt gcgtgcaacc tggttaagtt cgccagaagt tcccgtctgg ccgctacccg
    10501 cgaagaagat atgtgtagtg gggataagcg ggctgatgga tttccaggcg catcttgcgg
    10561 cggcttcgtt gcgcgaactg gatctttcgg ttgaaaccgc agaaatagag ctgccggaac
    10621 tggatgtcct gcgcaataac gccaccgaat ttcgcgcggt gaatatcgcc cgtttccttg
    10681 ataatgaaga aaactggccg ctgttacttg atgcgcttat tcctgtcgcc aatacctgcg
    10741 aaatgatcct gatgcccgcc tgcttcggcc tggccgatga caaactgtgg cgttggttga
    10801 atgaaaaact accttgttca ctgatgcttt tgccaacgct gccgccttcc gtgctgggca
    10861 ttcgtctgca aaaccagtta cagcgccagt ttgtgcgcca aggtggagtg tggatgccgg
    10921 gcgatgaagt gaaaaaagtg acctgtaaaa atggcgtagt gaatgaaatc tggacccgca
    10981 atcacgccga tattccgcta cgtccacgtt tcgcggttct cgccagcggc agtttcttta
    11041 gtggcggact agtagcggaa cgtaacggca ttcgcgagcc gattctcggc cttgatgtgc
    11101 tacaaaccgc cacgcggggt gaatggtata agggcgattt ttttgcgccg caaccgtggc
    11161 agcagttcgg tgtaaccact gatgagacgc tacgcccgtc gcaggcaggg caaaccattg
    11221 agaacctgtt tgccatcggt tcggtgctgg gcggatttga tcccatcgcc cagggatgcg
    11281 gcggcggtgt ttgtgccgtc agtgctttac atgccgctca acagattgcc caacgcgcag
    11341 gaggccaaca atgaacgaca ccagcttcga aaactgcatt aagtgcaccg tctgcaccac
    11401 cgcctgcccg gtgagccggg tgaatcccgg ttatccaggg ccaaaacaag ccgggccgga
    11461 tggcgagcgt ctgcgtttga aagatggcgc actgtatgac gaggcgctga aatattgcat
    11521 caactgcaaa cgttgtgaag tcgcctgccc gtccgatgtg aagattggcg atattatcca
    11581 gcgcgcgcgg gcgaaatatg acaccacgcg cccgtcgctg cgtaattttg tgttgagtca
    11641 taccgacctg atgggtagcg tttccacgcc gttcgcacca atcgtcaaca ccgctacctc
    11701 gctgaaaccg gtgcggcagc tgcttgatgc ggcgttaaaa atcgatcatc gtcgcacgct
    11761 accgaaatac tccttcggca cgttccgtcg ctggtatcgc agcatagcgg ctcagcaagc
    11821 acaatataaa gaccaggtcg ctttctttca cggctgcttc gttaactaca accatccgca
    11881 gttaggtaaa gatttaatta aagtgctcaa cgcaatgggt accggtgtac aactgctcag
    11941 caaagaaaaa tgctgcggcg taccgctaat cgccaacggc tttaccgata aagcacgcaa
    12001 acaggcaatt acgaatgtag agtcgatccg cgaagctgtg ggagtaaaag gcattccggt
    12061 gattgccacc tcctcaacct gtacatttgc cctgcgcgac gaatacccgg aagtgctgaa
    12121 tgtcgacaac aaaggcttgc gcgatcatat cgaactggca acccgctggc tgtggcgcaa
    12181 gctggacgaa ggcaaaacgt taccgctgaa accgctgccg ctgaaagtgg tttatcacac
    12241 tccgtgccat atggaaaaaa tgggctggac gctctacacc ctggagctgt tgcgtaacat
    12301 cccggggctt gagttaacgg tgctggattc ccagtgctgc ggtattgcgg gtacttacgg
    12361 tttcaaaaaa gagaactacc ccacctcaca agccatcggc gcaccactgt tccgccagat
    12421 agaagaaagc ggcgcagatc tggtggtcac cgactgcgaa acctgtaaat ggcagattga
    12481 gatgtccaca agtcttcgct gcgaacatcc gattacgcta ctggcccagg cgctggctta
    12541 aactcccttc tgatgcccgg taagcatgtg gttaccgggc atttttgcgt acacgattcc
    12601 gtgcccaatg tatgcgttgc aacgcagtga aaattcctct gaaaacgtct cgcaaaggct
    12661 gaaactggca gatgtcaaag gcctgggata accgtaatgt cgcgtcatca taaatatcag
    12721 gtgacggaca accatgaccg aatcaacaac ctcctccccg catgatgcgg tatttaaaac
    12781 ctttatattc acacccgaaa ccgcacggga ttttctcgaa atacatttac cagaaccact
    12841 gcgcaagctt tgcaacctgc aaaccttacg cctggaaccc actagtttta ttgaaaaaag
    12901 tttacgcgct tactactcgg atgttttgtg gtccgtggaa accagcgacg gtgacggcta
    12961 tatctactgc gtgattgaac atcaaagctc tgcagaaaag aatatggctt ttcggccaat
    13021 gcgctatgcc actgccgcca tgcagagtca cctagataaa ggctatgaca gagttccgct
    13081 ggtggtgcca ttgctgtttt atcatggcga aacatcgccc tacccgtact cacttaactg
    13141 gctggatgag tttgacgatc cgcaacttgc ccggcagttg tacaccgaag cttttccgtt
    13201 ggtggatatc accatcgtac ctgacgatga gatcatgcaa catcggcgta tagctctgct
    13261 ggaactgatt caaaagcata ttcgcgacca cgatttaatc ggcatggtcg acaggatcac
    13321 cacgcttttg gttagaggct tcactaatga cagccagcta caaacactgt ttaattatct
    13381 gctgcaatgc ggcgatacct cccgtttcac ccgttttatt caggagattg ccgaacgttc
    13441 accactacaa aaggagagat taatgactat tgctgaacgg ctacggcagg aaggacatca
    13501 aattggctgg caagaaggta aattagtagg tttgcagcaa ggtaaattag aaggtttgca
    13561 ggaaggtatg catgaacaag ccattaaaat tgctttgcgc atgctggaac agggcattga
    13621 tcgtgaccaa gtgctcgcgg ccacccagct aagcgaagcc gatctggcag cgaataacca
    13681 ctaattaaca caggcccaca gccgatcccc atgggccttt gatatcaata actacctttt
    13741 atgcgtggcc catttttgcc tgatttaaac atcgccagtc gttgatccag ggcatcgctg
    13801 tagagcatcg tgtcaacgcc aacagcgaca aagttcgctc cccacgccag gcattgctgc
    13861 gccatatcag gagccacagc cagaaaacca gccgctttac ccgcagcacg gatccgccga
    13921 atgctggttt caataattcg ctgcacttcc gggtgcccgg cgttatccgg gtagcccaac
    13981 gacgcagaaa gatccgctgg tccaataaac acgccatcaa tcccttcgac gtcgaggatc
    14041 tcgtccaggt tatccagtgc cgttttactt tccacctgca ctaacagaca aagcgaatcg
    14101 ttgacttgcg ccatgtaatt ctcaatgcgt ccccagcgcg cagcccgtgc cacactggcc
    14161 ccaacaccac gctcaccgta gggagggttg gacaaatttt gccggagggg gaatgtgccg
    14221 gtgcagtaaa ccggcacaga gtggcgaaat cagacggata aagtcatgga ctcaacaacc
    14281 tcaatccagc catgctcact agcgatgtcc tggccgttta accaacggcg tagcatattc
    14341 agtgccatca ttgcgcacac ttcctgacgg atagccaggc tgtagcgtgt agtgctgaaa
    14401 cgcacacgca gagcgaaagt gccgtctggc gtggctagcg caaagttgag atgctcgttc
    14461 tcgaaacccg aaacagccag tgccagcccg gcaaaatggt tggcccgccg ttctgtaatc
    14521 cagtgcgcag tttgcgccag ggtttcttcc tgtgaaggaa tcacttcaca cgccagcaat
    14581 ggagcacctg cgcgagaaag ttgcaaagcc aataaaccac cggtgaattg ctcgctcaac
    14641 gtcaggctga actggcggtt ttgcaattcg cgactgatct gcgcgggcag tccttcggtg
    14701 ccttcgaaaa tcacgctctg tccggcaaca cgtttaacgt ccagccacag tttttccatc
    14761 gcctgttgct cgcttgccgg tccggtgagt ttcagttcga tgatcggcat tgaggagcga
    14821 tagcccattg ttacgcccgg cggcagttgt agagtgtcca ggctttgtgc cagatcgctt
    14881 tccgaacgac caaaggtagt taaacgcaga caaaccggcg gctgtggtaa agaaaaacgc
    14941 tcgcgcaggc gcggcaggat ttcgtgctcg accatcacct taaattctga cggtacgccg
    15001 ggagtaaaga acatcaggca acgattaagc tgcacggcaa aaccacaggc ggtgcctacc
    15061 gggttattga taaattcagc actggcaggc agctccgctt gtttacggtt gctcggtgcc
    15121 attactcgtc cacgttcgtg gaaatagcgt tccatctctt tgagccaggc ttcatgcagc
    15181 accaggcctt caccttttgc tgtcgcagcg gcgagtgcgc ttaaatcatc gctggtcggt
    15241 cccagcccgc cgttaacgat cagcacatcg gcgtgctgac tacgttcgcg aagaatggtg
    15301 actaagtcat caaggttatc ccccaccgta ttgcggcgag ataatggcag cccctgatga
    15361 aagaaaaaat cggccagcca ggcagcgtta gtgtcaacga tttgcccgta taacacttca
    15421 tccccggtgg atagcatttc cacttttaac atggttatct cctgcttttg ttatcgaatc
    15481 actataacgc aaagggagat aaggcgtagt aagaaactga atgccgcgcc ggaagggatc
    15541 agaaactggc gttaacccca acatacaggc cgtcagccac agcgttgtcg cggttaccgt
    15601 ctttacccga cagattcagg tagcgataac ccgcctcaat actgactgga cgcataatgg
    15661 tgtaacgcgc gccagcattc gcttcctcat aactttgaat accgctggag agcgaatccg
    15721 gagagtagta atactcgcca aacaaacgga agctgttgcc aattttccac tgcaaaccac
    15781 ctcctaccgc tgcggcataa ccttcatcgc cgtaattcgg gttggtgtac acgccttttc
    15841 cgccaacggt cgccattaac ggcccgagag gcagattcaa ccccagcccc acgcccgcga
    15901 cgtcaccgtc gtcgtcgtta tgtgtccagt taccgcttaa agccaggccc gtcgattcag
    15961 tcccaaaacc gacaccaata ttggtgtatt ctttacccgc ctggccgctg atgctcattg
    16021 cattgaccga tgcagaaacc aacagcattc cggccagacc tgctagcgca atttttttca
    16081 ttatcccctt tcccacaagc aattaatcaa aattcccaaa gctgcgggat tgtactgcct
    16141 ggcacagagg attcaatgag cgaaaaaagg gatctagtca cttattgtaa ctaaatactt
    16201 cttaaataat taaacgccga aaaaataaac acactttacc gatacacaca aaaataataa
    16261 taagtgcagt tatcattgca aaagagatta ctgtcacttt cacatatttc tgagttaccg
    16321 tattctcatg gtttcttttt atttataagg aatacccctg tgcgacaacg gactattgta
    16381 tgccctttga ttcaaaatga tggtgcttat ttgctgtgca aaatggccga cgatcgcggc
    16441 gttttccccg gtcaatgggc gctttcgggt ggcggcgtgg agtctggcga acgaattgaa
    16501 gaggcactac gccgcgaaat tcgcgaagaa ctgggagaac agctgctttt gacagaaatc
    16561 acgccgtgga ccttcagcga tgatattcgc accaagacat atgcagatgg tcgcaaggaa
    16621 gagatttata tgatttacct gatttttgac tgcgtttctg ccaaccgaga agtgaaaata
    16681 aacgaagagt ttcaggacta cgcgtgggta aaacctgaag atctggtgca ttatgattta
    16741 aatgttgcca cccgaaaaac gttacgtttg aaaggtcttc tttaacaatg atgacagcca
    16801 tcactggctg tcagattgaa aatttagtgg ttaacgaatt ccccatccag ataaactttg
    16861 cctttttcca catgcatgac taaaccatcc agataatcag gtttaaatat cgcatcacgc
    16921 ttatttttag caatatatgt caggcaatga ttatgggtga aaataacgat atttttatca
    16981 ggcgctttgc tttgtaagtc cttaattgca ctataaatct cattaccgca ctgaagaagt
    17041 cgtttatcta ccgtcagctt tttacccgcg gaaaaccagg tcgccgactg aatggtccgg
    17101 acggtattac tggaataaag atcgaaatca gagatgtcag cgctaaaagc gttgcccagt
    17161 tcacgggcat cctgggtacc tttaaccgta atacctgttt tatctgacaa gcattgattg
    17221 gttgaacgat cgcaacgttc agcatggcga aacaaaacga caaccgggtg ctgctgtgcc
    17281 agtctggcca atgttttatt gtcgatacgt ggcaaaccat tgctactcca ggcggcatga
    17341 gtacccagtc cggcaattgc agcgagcgcc agtaaaatga tgaaatattt ttttgacttc
    17401 aatgatgagc ggcaaaaagc taacacgtaa actccaccta tagacaagcg caaccagaca
    17461 attaccgtga aattgagcga catttctggc gataattagc agttggtgta atattaaaaa
    17521 tcctacgatg tcggcaaaat gcctcaaatt tttgccaaat gcaaagccta aataagaaaa
    17581 aatataaaaa tttcaatatt tacgtctaat attagtttct taaggttaag ttaatattct
    17641 atccttaaaa tttcgctcca aatggcaaaa tatacacaac actctttata gcaaatataa
    17701 gtggacaggt attcaatggc ggaaggaaaa gcaatgtcag aatttttgcc tttttcgcga
    17761 ccagcaatgg gcgtggagga actcgctgca gttaaagagg ttctcgaatc cggttggatc
    17821 acaaccggtc cgaagaatca ggcgcttgag caagcctttt gccagttgac gggaaatcag
    17881 catgccatcg cggtcagttc agccaccgcc ggaatgcata tcacgctaat ggcgttggaa
    17941 attggcaagg gcgatgaagt gattacgcct tccctgacct gggtttcaac cctcaatatg
    18001 atttccttgc tgggtgcaac gccggtaatg gtggatgtcg accgcgatac gctgatggtc
    18061 acgcctgaag cgatcgaatc agccattacg ccacgcacta aagccatcat tccggtgcat
    18121 tatgccggtg cgccagcaga tattgacgcc attcgcgcca ttggcgaacg ttacggcatc
    18181 gcagttatcg aagatgctgc ccatgccgtc ggtacgtatt acaaagggcg acatattggc
    18241 gcaaaaggta ccgctatttt ttcatttcat gccattaaaa atattacctg tgctgaaggt
    18301 ggcctgattg taactgataa tgaaaacctt gcccgccagc tacggatgct gaaatttcac
    18361 ggtctgggtg tcgatgccta tgacagacaa acctggggcc gtgcaccgca ggctgaagtc
    18421 ttaacaccgg gctataagta caatctgacc gatattaacg ccgcgattgc cctgacacag
    18481 ttagtcaaat tagagcacct caacacccgt cggcgcgaaa ttgcccagca atatcagcaa
    18541 gcactggcag ctctcccctt tcagccatta agccttcccg cctggccgca cgttcacgcc
    18601 tggcatctgt ttattattcg tgtcgatgaa caacgttgtg gtatcagtcg cgatgcgttg
    18661 atggaagcgt taaaagaaag aggcattggt accgggttac atttccgcgc cgctcacaca
    18721 caaaaatatt atcgcgagcg ttttcccacg ctgtcgttac cgaataccga atggaatagc
    18781 gaacgcatct gttcgttgcc gctgttcccg gatatgacta ccgccgatgc cgaccgcgtc
    18841 atcacagccc ttcagcaact cgcaggacaa taagccatgt ttgaaatcca ccctgttaag
    18901 aaagtctcgg tggttattcc cgtttataac gagcaggaaa gcttaccgga attaatcagg
    18961 cgcaccacca cagcctgtga atcgttgggg aaagagtatg agatcctgct gattgatgac
    19021 ggcagtagcg ataattccgc gcatatgctg gtcgaagcct cacaagcgga gaacagccat
    19081 attgtgtcta ttttgattaa ccgcaattac gggcaacatt cagcgattat ggcgggattc
    19141 agtcacgtta ctggcgactt aattattacc cttgatgccg atctccagaa tccgccagaa
    19201 gaaatccccc gcctggtggc aaaagccgat gaaggttacg acgtggtagg gactgtacgc
    19261 cagaaccgcc aggacagctg gtttcgtaaa acagcttcga agatgattaa ccggcttatt
    19321 cagcgcacca ctggcaaagc gatgggtgac tacggttgta tgctgcgcgc ctatcgccgt
    19381 catattgtcg atgcgatgtt gcactgccat gaacgcagca cctttatccc gattctggcg
    19441 aatatcttcg cccgccgtgc aattgaaatt ccagtacatc atgccgagcg tgagtttggt
    19501 gaatccaaat acagttttat gcgcctgatt aatttgatgt acgacctggt gacctgcctt
    19561 accacaacgc cgctacgtat gctgagtctg ctcggcagca ttattgcgat tggaggtttt
    19621 agcattgcgg tgctgctggt gattttacgc ctgaccttcg gaccacaatg ggcggcagaa
    19681 ggcgtcttta tgctatttgc cgtgctgttt acttttattg gcgctcagtt tatcggcatg
    19741 ggattactcg gtgaatatat cggcaggatc tacaccgatg tccgcgcccg cccccgctat
    19801 tttgttcagc aagttatccg tccatccagc aaggaaaatg aataatgaaa accgtcgttt
    19861 ttgcctacca cgatatggga tgcctcggta ttgaagctct gctggctgcc ggttacgaaa
    19921 ttagcgccat ttttacccat accgataatc ccggtgaaaa agccttttat ggttcggtgg
    19981 ctcatctggc ggcggaaaga gacattccgg tttatgcgcc ggataacgtt aatcatccgc
    20041 tgtgggtgga acgcattgcc caactgtcac cagaggtgat tttctctttt tattatcgcc
    20101 atcttatttg cgacgagatt tttcagctcg ctcccgcagg tgcatttaat ctgcacggtt
    20161 cgctgttacc aaaatatcgt ggtcgcgcgc cgctgaactg ggtgctggtg aacggtgaaa
    20221 cggaaacggg cgttacattg caccgaatgg taaacgtgcc gatgctgggg ccattgtggc
    20281 gcaactgcgc gttgccattg cgccagacga tatcgctatt acgctgcatc ataaattgtg
    20341 ccatgccgcg cgccagctac tggaacagac attacccgcc attaaacacg gtaatattct
    20401 ggaaatcgcc cagcgcgaaa acgaagccac ctgttttggt cgcagaacgc cggatgacag
    20461 tttccttgaa tggcataaac cggcatccgt actgcacaac atggtacgtg ccgttgccga
    20521 tccgtggccg ggtgccttca gctatgttgg caatcagaaa ttcaccgtct ggtcgtcgcg
    20581 tgttcatcct catgccagca aagcacagcc ggggagcgtg atttctgttg cgccactgct
    20641 gattgcctgt ggcgatggcg cgctggaaat cgtcaccgga caggcgggcg acggcattac
    20701 tatgcagggc tcgcaattag cgcagatgct gggcctggtg caaggttcac gcttgaatag
    20761 ccagcctgcc tgcaccgccc gacgccgtac ccgggtactc atcctcgggg tgaatggctt
    20821 tattggcaac catctgacag aacgcctgct gcgcgaagat cattatgaag tttacggtct
    20881 ggatattggc agcgatgcga taagccgttt tctgaatcat ccacattttc actttgtcga
    20941 aggcgatatc agtattcatt ccgaatggat tgagtatcac gtcaaaaaat gtgatgtcgt
    21001 cttgccgctg gtggcgatag ccacgccgat tgaatatacc cgcaacccgc tgcgcgtatt
    21061 tgaactcgat tttgaagaga atctgcgcat tatccgctac tgcgttaagt accgtaagcg
    21121 aatcatcttc ccgtcaactt cagaagttta tgggatgtgt agcgataaat acttcgatga
    21181 ggaccattct aatttaatcg tcggcccggt gaataaacca cgctggattt attcggtgtc
    21241 aaaacaatta cttgatcggg tgatctgggc ctatggcgaa aaagagggtt tacagttcac
    21301 cctcttcctc ccgtttaact ggatgggacc acgactggat aaccttaatg cagcgcgaat
    21361 tggcagctcc cgcgctatta cgcaactcat tctcaatctg gtagaaggtt caccaattaa
    21421 gctaattgat ggcggaaaac aaaaacgctg ctttactgat atccgggacg gtatcgaggc
    21481 gttgtaccac attatcgaaa atgcgggaaa tcgctgcgac ggcgaaatta tcaacattgg
    21541 caatcctgag aacgaagcga gcattgagga actgggcgag atgctgctgg cgagcttcga
    21601 aaaacatccg ctgcgccatc atttcccacc gtttgcgggc tttcgcgttg tcgaaagtag
    21661 ctgctactac ggcaaaggat atcaggacgt agagcatcgt aaaccgagca tccgcaatgc
    21721 ccaccgctgc ctggactggg agccgaaaat tgatatgcag gaaaccatcg acgaaacgct
    21781 ggatttcttc ctgcgcaccg ttgatcttac ggataaacca tcatgaccaa agtaggctta
    21841 cgcattgatg tcgatgcctt tcgtggcacc cgtgaaggcg tgccgcgtct gctggaaatc
    21901 ttgagtaagc ataatattca ggccagcatt tttttcagcg tcggcccgga caatatgggc
    21961 cgccatctct ggcgactggt gaagccacag tttttgtgga agatgctgcg ctcaaacgcg
    22021 gcatcgcttt atggctggga tattttactg gcaggtacgg cctggccagg taaagagatt
    22081 ggtcatgcca atgccgatat cattcgtgaa gcggctaaac atcacgaagt cggtctgcac
    22141 gcctgggatc accatgcctg gcaagcccgt agcggtaact gggatcggca aacaatgatc
    22201 gacgatattg cacgcggtct tcgcactctg gaagagatta tcggtcaacc ggtaacctgt
    22261 tctgccgctg cgggctggcg tgccgaccag aaggtgatcg aagcaaaaga agcgttccat
    22321 ttgcgctaca acagcgattg tcgtggggcc ataccgttcc gtccactgct tgaatcagga
    22381 aaccctggca ctgcgcaaat tccggtgacc ttacccacct gggatgaagt gattggtcgg
    22441 gatgtgaaag cagaagattt taacggttgg ttactcaacc gcatcctgcg agataaaggc
    22501 acgccggttt ataccattca tgcagaagtt gaaggctgcg cttatcagca taattttgtg
    22561 gatctcctca aacgcgcagc tcaggaaggc gtgacatttt gccctttaag cgaactgtta
    22621 tcagagacgt tgccgctcgg acaagttgtt cgcggaaata ttgccggacg tgaaggctgg
    22681 ctgggttgcc aacaaattgc gggtagtcgc tgatgaaatc ggtacgttac cttatcggca
    22741 tcttcgcgtt tattgcctgc tattacctgt taccgatcag cacgcgtctg ctctggcaac
    22801 ccgatgaaac gcgttatgcg gaaatcagtc gagaaatgct ggcatccggc gactggattg
    22861 tgccccatct gttagggcta cgttatttcg aaaaacccat tgccggatac tggattaaca
    22921 gcattgggca atggctattt ggcgcgaata actttggtgt gcgggcaggc gttatctttg
    22981 cgaccctgtt aactgccgcg ctggtgacct ggtttactct gcgcttatgg cgcgataaac
    23041 gtctggctct actcgccaca gtaatttatc tctcattgtt tattgtctat gccatcggca
    23101 cttatgccgt gctcgatccg tttattgcat tctggctggt ggcgggaatg tgcagcttct
    23161 ggctggcaat gcaggcacag acgtggaaag gcaaaagcgc aggattttta ctgctgggaa
    23221 tcacctgcgg catgggggtg atgaccaaag gttttctcgc ccttgccgtg ccggtattaa
    23281 gcgtgctgcc atgggtagcg acgcaaaaac gctggaaaga tctctttatt tacggctggc
    23341 tggcggttat cagttgcgta ctgacggttc tcccctgggg actggcgata gcgcagcggg
    23401 agcctgactt ctggcactat tttttctggg ttgagcatat tcaacgcttt gcactagatg
    23461 atgcccaaca tagagctccg ttctggtact acgtgccggt catcattgcc ggtagcctgc
    23521 cgtggctggg attactcccc ggtgcactgt acacaggctg gaaaaaccgc aagcattccg
    23581 caaccgtcta tttgttgagc tggacgataa tgccgctgct gtttttctcc gtcgctaaag
    23641 gcaaattgcc cacctatatt ctttcttgct ttgcacctct ggcaatgctg ttggcgcatt
    23701 acgcgttgct ggcagcaaaa aataatcctc tggcgctgcg gattaatggc tggattaaca
    23761 tcgcttttgg cgtcactggc attattgcca catttgtgat ctccccctgg ggaccaatga
    23821 acacgccggt gtggcaaacc ttcgagagct ataaagtctt ttgtgcctgg tcgatttttt
    23881 cgctatgggc atttttcggc tggtacacct taacaaacgt cgaaaagacc tggtctttcg
    23941 ccgcgctttg cccgctgggg ctggcgttgc tggtaggatt ttcaattcct gacagagtca
    24001 tggaaggaaa acatccgcaa ttttttgtcg agatgacaca agaatcactt cagccaagcc
    24061 gctatattct tactgatagc gtcggtgttg ccgcaggtct ggcatggagc ctgcaacgcg
    24121 atgacatcat catgtatcgc cagacaggtg agttgaaata cggccttaat tatccggatg
    24181 cgaaagggag atttgtcagc ggtgatgagt tcgcaaactg gcttaatcaa catcgtcagg
    24241 aggggattat tacactcgtg ctttcggttg accgcgatga agatatcaac agtctcgcca
    24301 ttccgcccgc agatgccatc gttcgtcagg agcgtctggt gctgattcag tatcgtccca
    24361 aatgatctgg ctaacattag tctttgccag cttgcttagc gttgccgggc agttgtgtca
    24421 gaaacaggca acctgctttg tggcgataaa caaacggcgc aaacatatcg tgctgtggct
    24481 gggactggcg ctggcttgtc ttggtcttgc catgatgctc tggctgctgg tcttgcagaa
    24541 cgtaccggta ggcattgcct acccgatgtt aagtctgaat tttgtctggg tgacgctggc
    24601 tgcagtaaaa ctgtggcacg aaccggtatc gccgcgtcac tggtgtgggg tggcgttcat
    24661 tattggtggc attgtgatcc tcgggagtac ggtgtaatgt gcctgatatg gggattattc
    24721 agcgtcatta ttgcctcagt tgcgcaatta agcctgggtt ttgcggcaag tcatctgccg
    24781 ccgatgacgc acttatggga ttttatcgcg gctctgttag cttttggcct ggatgcccgg
    24841 atactgttac tcgggctgct gggttatctg ctttccgttt tttgttggta taaaacgcta
    24901 cataaacttg cgctaagcaa agcctatgcc ttgctcagta tgagttatgt gctggtatgg
    24961 attgcttcaa tggtacttcc cggctgggaa gggacttttt cgctgaaagc actactggga
    25021 gtagcctgta ttatgagcgg gttgatgctg attttcctgc ccacgacaaa acaacgttac
    25081 tgagttttcc ctgccgctgt acactgccgt tcccactcat cggggctata actgcttgcc
    25141 gagaggactt tcaccgtatg cagcttttca cgattaatac aataaagcgc attctgcaaa
    25201 ggcgagagta aatcccccac tttcacggcg aagtcactct tcacttcggc gatcattttt
    25261 atcgcgccgc cagcatcgca gagcataagt acatgtctgt tatcttgttt attgcagcac
    25321 gattttttgt gccagccatt ccattgcatt atcctgtttg ctaagagttt tcacatcaat
    25381 ttacagatta acagataaac tctttatcaa cggcaatgga atggcagaaa tgagaagtga
    25441 aacggagggt taattattgc tgacgttgca cccactcttt tagcgcctga cgtgaaattt
    25501 taataccgcc gtttttcagc tccggcggta aagtcagcca gcgcaccggt tgttgaaaac
    25561 gagccagctt atctttcacc cattcgccaa gatcaacggt ttgctggtca tactccacca
    25621 ccgctaccgg ccgatgacca aactccttgt cggcaacgga aacgataaac acctgcaaaa
    25681 ccgcaggatg tgcagcaata acacgctcga cttcttccgg ctgaataccc tcaccgccac
    25741 tgaagaacag attgtctaaa cgtccgacaa tggtcagctt gccattatgc atctcaccgc
    25801 gatcgcgcgt agcgtaccag ccttcgtcat taaccagtga aaccagttgc ccgttacgcc
    25861 agtaaccttc tgccatactg gcagcccgca gccacacttc attattaacg attttcactt
    25921 cccgacccgg cagcggcaaa ccaacgtctg ccaggccgtc ggcttctttc gcacataccg
    25981 tggaggcaaa ctcggtcaga ccatagccgc aaaagcaacg aatcccctgc tcgcgcgcct
    26041 gttccgtcaa ctcgaccggg atagccgcgc cgccaagtaa caccgctttc agggaaacgg
    26101 aactgcggtt aaccagcaaa cgccagagtt gtgttggcac cagtgaagcg tgagtacagc
    26161 ctgccagcat ttgctccaat ggctgtttat cacgcaccgt catccgcgca ccagcgtata
    26221 accagcgcca cataattccc tgaccggaga cgtgaaacag cggtaaagag agcaaccagt
    26281 catcgtgatc gccaaacgga atcagcgata acacaccttg cgcactggca agatgggcct
    26341 gataagtatg gacagcggct ttcggcaaac cggtagaacc cgaggtcaac gtcattgagc
    26401 acagacgcgt cggctgccac gcagcggcat gtacgccttc aaccagctga atgtgcagcg
    26461 acgttaatgc cggaaacgtg ttttccccat ccggcaccag cgcaaattgc agcgtcagat
    26521 tgggcagcaa ttcttcaagc aacggttgcg gcagctgagg gttcacgggc aacacccgcg
    26581 ccccgcattg cagtaacgcc agccaggcga gcagcgtttg cggcgtattc cacgcccgca
    26641 acatcacgcc gctgccctca accaccccct gcgccgcaaa gcccgtagct aactcatcga
    26701 cgcgagcaca aagctcgcgc cagttgagtt gctcgtcatt aagacgtaag gcgatggctt
    26761 ctccccgcac ttgccgccag tgacgccacg gccagtcaga gaagatcata acaactgctc
    26821 ctgtacatca gcgtccacta acggcagtgg gctccccggc cagcgacgaa cctgctgcgc
    26881 ctgcatcaga tccagcgtgt ccagtcccgg aatggtgtct ggcgttaacc aggcagcaat
    26941 ccgcgccagt tgcgttagac ctaagctcga ctcaatggaa gaactgatca ccgccgtcag
    27001 ccccagcgca tgcgccgcct gtacctgctc gcgtactttt tccagactgc ccgtgagcgt
    27061 gggtttgata actaccgcgc gcacgccctc ttcagccaca aaggcaaaat ccggctcgcg
    27121 caggctttca tcccaggcaa tggcaatgcc ggtttcacgg gcaaacgctc gcgaatcatc
    27181 gcgggttttg cacggctctt cgagaaacgc gatgcggtgg cgataatccg ggttaacata
    27241 tttggcaaac tgctgacctt tcagcggtgt ccaggcgcga tttgcgtcaa gacgcaaatg
    27301 cagatccgga attgcctcca gcaacagatt caccaccatg ccgtcgcgca ccgcttcgta
    27361 caatcccact ttgaccttcg ccactttctc gcccggcata tcggcaagtt tgaggatcag
    27421 atcgtccgga tcgccattac acagcggtgc cgcacggtag ttggctgctt gcggcaacgt
    27481 atctgtcagt tctgccaatg cacagcttac gccaaaggcc acggaaggca tctgcggtag
    27541 ctcgcaatcg cctgccagcc agttatttac ccaggcaagc agcacgcttt gcgcctcttc
    27601 ccagttttcc tggctgaagc ccggcagtgg ggagatctcc ccccaccctt cgcgctcgcc
    27661 ctcacgcagg caaacgtaca gcccgtcgcg ggtttttaac cgcctgtcgc gcagaaccac
    27721 ccccgcgtcc atggggatct gccagcggta tacctgcgcg ctacgcatta cggattccgt
    27781 ttaaatttgc tgaaatcagg ctgacgtttc tggttgaaag cgttgcgacc ttcctgacct
    27841 tcttccgtca tgtagaacag catggtggcg ttgcccgcca gctcctgcag ccccgcctgc
    27901 ccgtcacagt cggcgttcag cgcagctttc aggcagcgca gcgccatcgg gctgttttgc
    27961 agcatttcgc ggcaccaacg gacggtttct ttttccagat ccgccagcgg taccacggtg
    28021 ttgacaaggc ccatatccag cgcctgtttt gcgtcgtact gacggcacag gaaccagatt
    28081 tctcgcgctt ttttctgccc gacgatacga gccatgtagg aagcgcccca gccgccgtcg
    28141 aaggaaccga ctttcgggcc agtctgaccg aagatggcat tatctgctgc gatagtcagg
    28201 tcgcacatca tgtgcagaac gtgaccaccg ccgatggagt agccagccac catcgcgaca
    28261 accggtttcg gacaggtacg gatctgacgc tggaagtcca gcacgttcag gtgatgtacg
    28321 ccggaatcat ctttatagcc gccgtaatca ccacgcactt tctggtcacc accggagcag
    28381 aacgctttat cgcctgcgcc agtcagaatg atcacgccga tattgtcgtc ataacgcgca
    28441 tctgccagtg cctggatcat ctcttttacc gtcagaggac ggaaggcatt gcgcacctgc
    28501 ggacgattaa tggtgatttt tgcgataccg tcggtggatt tttcataacg aatgtcctcg
    28561 aaaccttcgg agcagtcgtg ccattcaacc ggtgcgtaag gcattgcttc atcaggataa
    28621 atcatattgt gtcctttagt cagaaacgca agatctgcgc cagacttgcg attacgccag
    28681 cgggattttc ccgatgcgcg ttatgtccgg cgcgaggaat gacatggcag tcggcagcca
    28741 gttccgccgc cagggcgcgg aatttgctgt cacgttcacc acataaataa taaaacgcaa
    28801 atgtgcgggc gctaaggtta gcacgtaaat caggctggac ggcgagagaa gtcgcctcca
    28861 gcatggcggc aagggttgcg ccattattgt tgctgcgcag cgccaccagc tcccggcgtt
    28921 gatcgtcatt gagcgaggca aaaacaggct gttgatacca gtcggcaaat accgccgtta
    28981 acggttctgt gcgaaaacgc tgcgcccatt ggcgatcgga acgctgacgt tccgcacgtt
    29041 gttcagcatt ttgcagcccc ggatgcccgc cttcgacaat aaccccacaa agccccgcca
    29101 gcccctggca agccgccatc atcgccaccc gtccgccaag cgagtacccc accagccaga
    29161 agtcaaggat gttgtaacta accaaggttt tacgcagtaa gtcggtgaca tcatcaaatc
    29221 catcgacgct aatcgccgcc gaaccaccat gacctaggag atcaacatac aaccgtgagt
    29281 agtcggcaaa cgcctcgccc acttcttgcc attcgtggca atcgccggaa aaaccgtgca
    29341 aaaacaccag ccagggtaaa cctagttttc cgtgttttgc ctgcgcgtgc aggatcataa
    29401 atggcttacc tgcgccagaa gttgctggag cgtttgcgca ccatcggtgt cgttaaccac
    29461 catttcaatc accgtggtgg ttggcgtgcg ccaggcgtcg gcaaatgccg tttcaagttc
    29521 ctgccagttt tgcggatgat gatatttcag ctcgaacatc gcggcggcgt gctcaaaatg
    29581 gacgttttgc ggcatcagat agaaacgctc acgctcgctt tgcggcgttg gcaacagcga
    29641 gaaaatttgc ccgccgttgt tgttcaccac aattaatacc agcggcgcag aaacctgacg
    29701 caataacgcc agcgcgttga gatcgtaaag cgcggagaga tcgcccacaa tcgccagcgt
    29761 cggtttgccg cttgcccgct gaacgcctgc ggcggtcgaa agcagtccgt cgataccgct
    29821 ggcccctcgg ttgctgtaca ccgggtaacc tgccggaagt tgcgaaagcg catcaatcag
    29881 acgtaccacc aggctgttac caacaaacaa ttgcccctgt tcaggcagat agtcgctgat
    29941 gcgatgcgcc agttgcgctt cgccaaacgc atcacggcgg gcaataaccg cctgcattgc
    30001 ctgttccgcc aggcgcggga tttcaacgca ccagggctgg cgtttttctg ccggatgcag
    30061 ctccagccag tcggcaatat tggcaattaa gcgacgtccg cgatggtgtg ccggatcaag
    30121 tcgcccttca atgtcatcaa caatccagta ctcttctggt tcacagcttg cctgccattg
    30181 caggagccgt ttccccgtca ggctacttcc cagttgcacc acaatttgcg cctgctgcag
    30241 ctcgctggtc gctttggcat tgcctaacca aagatcggca cacggcagcg gctgcccggt
    30301 ttgtgacagc acatcgccaa tcagcggcca gccaagagtt tgcgcccaca gggcaacttt
    30361 tttgccctct tccgcactca tgcgcccggc aaccaccacg ccgcgctttt gtcgccagaa
    30421 gaaccagtcg cgctgttttt cactttccag acgaggcgct tcacgcagcc acggtttgtc
    30481 gtcctgccac cagtcaccca gacgctgttg ccagctaagc ccggtatcgt ccatttcgcc
    30541 atacagcggt tcagcaaacg ggcagttgat atggactccc cccgcatgaa gcgtaccgag
    30601 agcgtggtcg atggtagaaa ccagccaacg tgcggggata tcctgggtcg ggcgcggcaa
    30661 tgaaatactg tgcgtggggt gagaggcgaa cattcccggc tggcgaattg cctgattcgc
    30721 gccgcagtca attagctccg gcgggcgatc ggcggttaag agaatcagtt tttctccggt
    30781 taacccggct tcaatcagtg ccggatagag atttgccacc gccgtgccgg aggtcacaat
    30841 caccgccacc ggctgcttgc tgactttcgc cagccccagc gccagatgcc ccaacccacg
    30901 ctcatcgaaa tgggtgtggt gaatgaatgc ggaattctcc gccgccgcta acgttaacgg
    30961 tgtagaacgc gagcctgggg cgatacagat gtgtctgacg ccgtgacgcg ttaatgcttc
    31021 cagaatgacc gccgcccagc gtcggttaaa tgcgcttact gacatgagtt tgtccggtat
    31081 caatattgcg gctaagtata aggagctaca aaaatagatt attgatatga atcggtaatg
    31141 atgcgactca ttactattcc atttgtaata aagtacgcag ccctgccgct ttgttgtcga
    31201 tttcctgcca ctcttgctcg gggtcggaac cacggacaat gcccgcgcca gcatataatc
    31261 gcacgacatt gccgctaatt tttgctgagc gcagggaaac gcagaattcg ctttgttgta
    31321 atgagagata gcccgcagaa ccggcgtacc attctcgggt gaacggttcg tgacgggcga
    31381 taaactgtcg cgccagatcg cgcggtaagc cagcaactgc tgccgttggc agcaactgat
    31441 gtaaacagat cacatcatcc gctttgttga gtgaagtcca gatacagcgg cgaagatgct
    31501 gcactttacg cagacgcagt acctgcggcg gtaaaacatc cagcgtctgg gtatcggcct
    31561 gtaatcgttg gcagatatct tccaccacca gcatgttctc gcgctggttt ttatcatccg
    31621 ccatcagcca ctctcctaac tgctgcgcct gcttatcatc aggatgattt gctactgttc
    31681 ccgccagcgc ttcagtacgc agcgctttgt cacgccgccg ccataaccgt tccggtgaag
    31741 agccaagaaa agcattttcg ccatcaaagg ccatgtaaaa atggtagcaa ttcagattca
    31801 gtcgacgact ggcagccatc atcgccgccg cgttgaccgg acttgcgaaa tgcaggtcag
    31861 ttgcccgagc gagcaccact ttgtcgagct caccttcggc gatggttttc gttgccagtt
    31921 cgattaattg cgtccagccc gttttgtccg gccagtgttg ttctcgcgtg gtggttaaat
    31981 gtaacccagg caagggcttg atactcacca gtgtggcgat aaattctttt gcctgaatcg
    32041 catcgtgctg aagggagctt tcgctgaata gcgtcagccg cagcgtggct ttaccgccac
    32101 agcgtcgcca ttccaggcgg ggtaaaagta aattgccctg cgacgggtca aaagcattca
    32161 gcccccaaat gcgtaagtca gcgtgttccg ggtgctggcg aagaaaacgt tgtgcctggt
    32221 ccaacgacgt aaaacgggta atcgcgccca ggacggcagc ttcttcatca ccattacgtt
    32281 gttgccagta gaattgcggg tacgtttgct gactggccag ccagctcaag gcatcaaaag
    32341 cgtctttgag agggaaagga atatcgataa cccgaatgcc gggtgtcgcc ggaatctctt
    32401 gcgacaaatg gcgcagtaga ttttccagcg ccgtagtaag tgattgcacg cggacctctc
    32461 cctgttaaaa gcctcacatt atacggggta ctacaaaaaa aatgcagtac cccggtgtag
    32521 ggaggtttta acggcgtgcc agcaacagtc ctagtaccag cccgacggcc gcgcccacac
    32581 caattccttg ccagggtttt tcgtggacgt agtcatcagc acgataaaca gcctgcttcg
    32641 cccgatagta ataactgtct gaagcctggc taacccgttt tttcacatca tccagcgctt
    32701 tttctgcatg cgctttcagc tcaacatatt tctgatcggc gggatcgcca gaggagcgga
    32761 gcacctcttc cagtgtttca ctaagcagcg tcaggtcgtc atcgatacgt gtatcaccaa
    32821 actgattaga cattctcgtt ctccatttgc gtaaaacctg tcactaacta tagacaagag
    32881 ttgttgatta cgcctgaatt acctcgcgtg ccatcccaat gtgtgggata ccatcttctt
    32941 catagacctc cgtcacaggg ataaaaccga aactctggta gaagttttgc agatgcgcct
    33001 gcgccccgag gtaaacaggc ttatcaggcc agtgatgcgt acatgtttcg agtgttttac
    33061 tcatcagctg ctgccccact ttttcaccac gcaacgcttc actgacaatc acccgaccta
    33121 taacgaccgg ctcaagatca tcatcacttt tcagaatcct cgcatacgcc accagttcat
    33181 catttttcca accgagaata tggcgattat cccccgtcaa atcatcacca tcgatatcct
    33241 gataagggca attctgctcg acaacaaaca ccgcgcaacg cagttgtaat aaggcataaa
    33301 gctgggaaac cgataattca gagtgatgca gatcttgcca ttcaatcata atgctctcct
    33361 tttagatgta tgccttatac tagccggaat attttttgaa acgtgatgaa ctcatggaat
    33421 taattttttt aggtacttca gccggtgtgc caacccgcac gcgcaatgtc acggcaatat
    33481 tgcttaacct gcaacatccg acccagagcg gactttggtt gtttgactgc ggtgaaggca
    33541 cccagcatca gctactgcat accgccttta accctggaaa actggacaag atttttatca
    33601 gtcaccttca tggcgatcat ctttttggtt tacccggctt gctgtgcagt cgttctatgt
    33661 caggcattat ccaaccctta acgatttatg gtccgcaagg tatccgtgaa tttgtggaaa
    33721 ccgcgctgcg gattagcggc tcatggaccg attatccgct ggaaattgtc gaaattggcg
    33781 ctggcgaaat tctcgatgat ggcctgcgca aagtaaccgc ttatccgctg gaacacccac
    33841 tggaatgtta tggctatcgt attgaagaac atgataaacc gggtgcatta aatgcccagg
    33901 cattaaaagc tgctggcgtg ccgcctggcc cactgtttca ggaattaaaa gcgggcaaaa
    33961 caatcacgct ggaagatgga aggcagatta acggcgcaga ttacttagct gctccagtgc
    34021 caggtaaagc gctcgctatt ttcggcgata ccggcccctg cgatgccgca cttgacctgg
    34081 ctaaaggtgt cgatgtcatg gtgcacgaag cgacgctgga tataaccatg gaagccaaag
    34141 ccaatagtcg cggccatagc tctacacgcc aggctgcgac actagcccgt gaggctggag
    34201 tcggcaagct aatcattacc cacgtcagct cgcgctatga tgacaaaggt tgtcaacacc
    34261 tgttacgtga atgcaggtca attttcccgg cgactgaact ggcgaatgat ttcaccgtgt
    34321 ttaacgttta actctgctta ttattaaaaa cagggtgaaa cttgccctgt tatagcaacc
    34381 cgcgctacat atatccaccc acacaggcat attgagcgat ttttgatatt tattcatctc
    34441 cttacgcact atattcagag caataagttg cctcttttca tcttgattca gcacaacctt
    34501 tgcaactaaa aggagcccat gatggttaca gttgtcagca attattgtca attatctcaa
    34561 acccaactca gtcagacatt tgcagaaaaa tttactgtga ccgaggaatt actgcagtct
    34621 ttaaaaaaaa cagcgttatc cggagatgaa gaa
//
LOCUS       DAJTQQ010000036        34468 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000036 DAJTQQ010000000
VERSION     DAJTQQ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34468
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.036"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..334)
                     /locus_tag="OO309_001913"
     CDS             complement(<1..334)
                     /locus_tag="OO309_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5847068.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQK"
     gene            704..1654
                     /gene="corA"
                     /locus_tag="OO309_001914"
     CDS             704..1654
                     /gene="corA"
                     /locus_tag="OO309_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR5847069.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1695..2075)
                     /locus_tag="OO309_001915"
     CDS             complement(1695..2075)
                     /locus_tag="OO309_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCR5847070.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2089..2505)
                     /locus_tag="OO309_001916"
     CDS             complement(2089..2505)
                     /locus_tag="OO309_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5847071.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2564..3454)
                     /gene="rarD"
                     /locus_tag="OO309_001917"
     CDS             complement(2564..3454)
                     /gene="rarD"
                     /locus_tag="OO309_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR5847072.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3506..3973)
                     /locus_tag="OO309_001918"
     CDS             complement(3506..3973)
                     /locus_tag="OO309_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR5847073.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4138..5007
                     /gene="pldA"
                     /locus_tag="OO309_001919"
     CDS             4138..5007
                     /gene="pldA"
                     /locus_tag="OO309_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR5847074.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5140..6969
                     /gene="recQ"
                     /locus_tag="OO309_001920"
     CDS             5140..6969
                     /gene="recQ"
                     /locus_tag="OO309_001920"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR5847075.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7033..7653
                     /gene="rhtC"
                     /locus_tag="OO309_001921"
     CDS             7033..7653
                     /gene="rhtC"
                     /locus_tag="OO309_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR5847076.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7715..8335)
                     /gene="rhtB"
                     /locus_tag="OO309_001922"
     CDS             complement(7715..8335)
                     /gene="rhtB"
                     /locus_tag="OO309_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR5847077.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8446..9468
                     /gene="pldB"
                     /locus_tag="OO309_001923"
     CDS             8446..9468
                     /gene="pldB"
                     /locus_tag="OO309_001923"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR5847078.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9476..10276
                     /gene="yigL"
                     /locus_tag="OO309_001924"
     CDS             9476..10276
                     /gene="yigL"
                     /locus_tag="OO309_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR5847079.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10352..11251
                     /gene="bioP"
                     /locus_tag="OO309_001925"
     CDS             10352..11251
                     /gene="bioP"
                     /locus_tag="OO309_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR5847080.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11139..12092)
                     /gene="metR"
                     /locus_tag="OO309_001926"
     CDS             complement(11139..12092)
                     /gene="metR"
                     /locus_tag="OO309_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR5847081.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12328..14589
                     /gene="metE"
                     /locus_tag="OO309_001927"
     CDS             12328..14589
                     /gene="metE"
                     /locus_tag="OO309_001927"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR5847082.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14628..15443)
                     /locus_tag="OO309_001928"
     CDS             complement(14628..15443)
                     /locus_tag="OO309_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5847083.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15705..16466
                     /gene="udp"
                     /locus_tag="OO309_001929"
     CDS             15705..16466
                     /gene="udp"
                     /locus_tag="OO309_001929"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR5847084.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16607..18034
                     /gene="rmuC"
                     /locus_tag="OO309_001930"
     CDS             16607..18034
                     /gene="rmuC"
                     /locus_tag="OO309_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR5847085.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18129..18884
                     /gene="ubiE"
                     /locus_tag="OO309_001931"
     CDS             18129..18884
                     /gene="ubiE"
                     /locus_tag="OO309_001931"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR5847086.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18898..19503
                     /gene="ubiJ"
                     /locus_tag="OO309_001932"
     CDS             18898..19503
                     /gene="ubiJ"
                     /locus_tag="OO309_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR5847087.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19500..21140
                     /gene="ubiB"
                     /locus_tag="OO309_001933"
     CDS             19500..21140
                     /gene="ubiB"
                     /locus_tag="OO309_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR5847088.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21219..21488
                     /gene="tatA"
                     /locus_tag="OO309_001934"
     CDS             21219..21488
                     /gene="tatA"
                     /locus_tag="OO309_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR5847089.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21492..22007
                     /gene="tatB"
                     /locus_tag="OO309_001935"
     CDS             21492..22007
                     /gene="tatB"
                     /locus_tag="OO309_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR5847090.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22010..22807
                     /gene="tatC"
                     /locus_tag="OO309_001936"
     CDS             22010..22807
                     /gene="tatC"
                     /locus_tag="OO309_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR5847091.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKAEAESEKTEE"
     gene            22849..23631
                     /gene="tatD"
                     /locus_tag="OO309_001937"
     CDS             22849..23631
                     /gene="tatD"
                     /locus_tag="OO309_001937"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR5847092.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23628..24116)
                     /gene="rfaH"
                     /locus_tag="OO309_001938"
     CDS             complement(23628..24116)
                     /gene="rfaH"
                     /locus_tag="OO309_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR5847093.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24283..25776
                     /gene="ubiD"
                     /locus_tag="OO309_001939"
     CDS             24283..25776
                     /gene="ubiD"
                     /locus_tag="OO309_001939"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR5847094.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25822..26523
                     /gene="fre"
                     /locus_tag="OO309_001940"
     CDS             25822..26523
                     /gene="fre"
                     /locus_tag="OO309_001940"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR5847095.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26715..27878)
                     /gene="fadA"
                     /locus_tag="OO309_001941"
     CDS             complement(26715..27878)
                     /gene="fadA"
                     /locus_tag="OO309_001941"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR5847096.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27888..30077)
                     /gene="fadB"
                     /locus_tag="OO309_001942"
     CDS             complement(27888..30077)
                     /gene="fadB"
                     /locus_tag="OO309_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR5847097.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30267..31598
                     /gene="pepQ"
                     /locus_tag="OO309_001943"
     CDS             30267..31598
                     /gene="pepQ"
                     /locus_tag="OO309_001943"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR5847098.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31598..32212
                     /locus_tag="OO309_001944"
     CDS             31598..32212
                     /locus_tag="OO309_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR5847099.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32251..33702
                     /gene="trkH"
                     /locus_tag="OO309_001945"
     CDS             32251..33702
                     /gene="trkH"
                     /locus_tag="OO309_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR5847100.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33714..34259
                     /gene="hemG"
                     /locus_tag="OO309_001946"
     CDS             33714..34259
                     /gene="hemG"
                     /locus_tag="OO309_001946"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR5847101.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8119 a         8703 c         9308 g         8338 t
ORIGIN      
        1 ccttctgctg accgttttcg atgtacaaac cgagcggcgc atagctttca tcatagatgc
       61 cgccgttcat cgccatctgc acctgaccct gactattaat atccgccagc agagcatgta
      121 acgttcccca cgcttcgcca ttggcttttt gccagtacat tttcacccgc tctgtttgag
      181 gattaacggt atacgcctgt acggtcagcg tcggatctga gagtgcgcaa tcatcagcgg
      241 caacagcaaa caagggaagt aaggtgaggg tgagaaaaat ccgtttgaga ttcaaggtga
      301 tcattccttt accaatgagt agccgatgcg ccattatagg tcctagatgt gggatttttt
      361 tatcctgtta gcgaccttga cgagtaccaa aaagcgcgaa gttcaactat tgttctgtgg
      421 tgttctgttg cgtgttgacg gcaaaatttt gctggcgtaa catgcgcgca cgatcactct
      481 aagaggacat tcgccttgga cacacccagt agatactggc tcactatcct gtcatccagg
      541 atcaactcct aaggctatcc ctttttgctg atagccttag cggttgtcag cgacctcaat
      601 ttttcccgtc gcgctgagtc aggctgttta atggtctgaa acccaatttg tttctgtgtg
      661 cccaccgaac tgtccgatat tttaagcatt gggagtcccg gtcatgctga gcgcatttca
      721 actggaaaat aaccgactga cccggctgga agtcgaagag tcacaacccc ttgtaaatgc
      781 agtatggatt gatcttgtcg aaccggacga cgacgagcga ctgcgcgtac aatctgaact
      841 tggccagagc ctggcaaccc gcccggaact ggaagacatc gaagcatcgg cacgtttctt
      901 tgaagacgac gacggcctgc atattcactc cttcttcttc tttgaagatg cggaagatca
      961 cgccggtaac tccactgtgg catttaccat ccgtgatggt cgtctgttta ctctgcgtga
     1021 gcgtgaactg cccgcttttc gtctgtatcg tatgcgtgcc cgtagccagt cgatggtaga
     1081 cggtaacgcc tacgagttgc tgctggatct gttcgaaacc aaaatcgaac agttggcaga
     1141 tgaaattgaa aatatctata gcgacctgga gcagttgagc cgggtgatta tggaagggca
     1201 tcagggcgat gagtacgacg aggcgctctc cacgctggcg gaactggaag atatcggctg
     1261 gaaagttcgc ctgtgtctga tggataccca gcgcgctctc aacttcctgg tgcgtaaagc
     1321 gcgtttaccg ggtgggcaac tggagcaggc gcgtgaaatc ctgcgcgata tcgaatccct
     1381 gctgccgcat aacgaatccc tgttccagaa ggtgaacttc ctgatgcagg cggcaatggg
     1441 ttttatcaac atcgagcaga accgcatcat caaaattttc tcggtggtat ccgtcgtgtt
     1501 cctgccgcca acgctggttg cttccagcta cgggatgaac tttgagttta tgccagaact
     1561 gaagtggagt ttcggctacc ctggcgcgat tatctttatg atcctcgcgg gcctggcacc
     1621 gtatctgtac tttaagcgga agaactggtt gtaaaaagaa ggcggtggct tagcctggct
     1681 aagccacctg ttattcaaag gctccaggta tttaaccctt ttacctcttt ctcataaaac
     1741 catttgttcg tgttaacagc aacataggct gctacggcaa ttcccagaat gttaacgcca
     1801 attaatgcac caacaaatag agatagaatg ccaagtaata gaactataat tgcttttttc
     1861 cataagccca gaacaaataa atagatccag gaacagaaga aagcaatgaa attcatttga
     1921 atagttaatc gttgtcttac ttttaatgct ttaaatgctg ctttatattc tggtgttgct
     1981 ccccagaatc caggaaagcc atgttgatca taaaaattaa atcggtattt ccatttttca
     2041 cttaatgaat catcgttcat atattcctta ctcataaata ctccataact attgttttga
     2101 tgaatcagta ggtgcaagca ttagcatact gaaagtggaa aaataacaaa tcaaaaaaat
     2161 catcgaacca ttgcctgaac aggcaaaatc ttcggctatc attgtgatga tagagatgat
     2221 atatactgct aatgtaccaa aaacataaat ttttatatat atgaaaccac tatcacggag
     2281 tcgttggcaa ttcatgttga tgacgagata atggagtgcg atggtagaga ctataacaag
     2341 aaaacctgct tccccatcgt taaaaaagat aataagaaag gcaaaaatga aatttattaa
     2401 aataaataaa aatatatatc ggtgtctgga aatcttaccg ttagatgttg ggataaacat
     2461 acgtaacata aattttacat ccttgtgtga gtctcctgtc agcatggcaa tatgcccact
     2521 ctcatacaag agtcggcata ttttttcaga atatatttat tttttatttg gacgttctac
     2581 gctgcgtata aatcgcgtcc atcacaaaaa ttgccagcgc cacccaaata aaggtgaaag
     2641 tcaccatctt atcggcaccc ggtttttcac cataaaacgt cacagccagc aggaacatca
     2701 gcgtcgggcc aatgtactga aaaaagccta acgttgagag acgcaggcgc gtggcagcgg
     2761 cagtaaaaca caacagcggt acggtagtga caataccggc ggcgatcagc agtaaattca
     2821 gcgacatcgg gttttgcccc atatggctgg ttgaactgtc ggcaatagca aacaggtaaa
     2881 tcgccgccac gggcagcagc cacatggttt caattaacat gccggtttgt gcttctaccg
     2941 caatcttctt gcgcaccagg ccgtagaagg caaaactaaa tgccagcccc agcgcgataa
     3001 taggtagcga accaaaagtc cacagctgga ccaacacgcc acatatcgcc agaatcaccg
     3061 ccagccattg catccggcgg aatcgctcgc cgaggaaaat catccccagc acaatgttca
     3121 ccagcgggtt aataaagtaa ccaaggctcg cttccagcat atggtgattg ttcaccgccc
     3181 agataaacag tagccagttg ccaccaatca gtacggcaga gactgccagc ataaaaattt
     3241 tctgtggcgt ctgaatcagc gtttttaaat aggaccactg gcggcaaatg ctcatcagca
     3301 ccaccataaa gaaaaacgac cagatcacgc gatgcgtcag gatctcatcg gcgggcacgt
     3361 agtaaatcag cttgaagtat gctggcgcaa taccccaaat aaaataagcg gcaagagcga
     3421 gtaatacgcc ctgccgcgtt tgttttgcat ccatcgggaa tactcatttt taattggtaa
     3481 cagcagttta cctgctttta tgtcttcaac ctaccatata ggtggcggtg gcactggcaa
     3541 tataaagctg ttcttcattg tgtaattcaa cgcgggcgac ggcgacttta ttgcctgcac
     3601 gcaacaggct actggtagca gtaaaacgct cgcccctgcc tgggcgcaga taatcaacgc
     3661 gaagatcaat ggtccccatc cgcgatagcc gctggcgtag ttcatcttca ctgatggttt
     3721 cgtggcgggt taaggtactt cccacgcaca ccagaccggc ggcgacatcc agcgccgacg
     3781 caatgactcc gccgtgcaaa atgctttgcg cccagttacc caccatcatc ggctgatttt
     3841 taaaggccag ctgtgcgaac tctttttcgt aacgctccag ttccatcccc aatgcgcggt
     3901 taaatggcat gtgataaaca aacatctcac ccactaattt cagggcttgt tcagcggtca
     3961 gtacggcaga catatcatcc ttacacttca ttggttaatg aaatgttgat tttatgcttc
     4021 tttgttgttg gtttctactt taggaaggga taactaacgg caatggagtt aagtatgtaa
     4081 aatagcccgc agaaaaacat tcaccttatc aataattcgt tacggagaac acgaccgatg
     4141 cggactctgc agggctggtt gttgccggtg tttatgttgc ctatggcagt atatgcacaa
     4201 gaggcaacgg tgaaagaggt gcatgacgcg ccagcggtgc gtggcagtat tatcgccaat
     4261 atgctgcagg agcatgacaa tccgttcacg ctctatcctt atgacaccaa ctacctcatc
     4321 tacacccaaa ccagcgatct gaataaagaa gcgattgcca gttacgactg ggcggaaaat
     4381 gcgcgtaagg atgaagtaaa gtttcagttg agcctggcat ttccgctgtg gcgtgggatt
     4441 ttaggcccaa actcggtgtt gggtgcgtct tatacgcaaa aatcctggtg gcaactgtcc
     4501 aatagcgaag agtcttcacc gtttcgtgaa accaactacg aaccgcaatt gttcctcggt
     4561 tttgccaccg attaccgttt tgcaggttgg acgctgcgcg atgtggagat ggggtataac
     4621 cacgactcta acgggcgttc agacccgacc tcccgcagct ggaaccgcct ttatactcgc
     4681 ctgatggcag aaaacggtaa ctggctggta gaagtgaagc cgtggtatgt ggtgggtaat
     4741 actgacgata acccggatat caccaaatat atgggttact accagcttaa aatcggctat
     4801 cacctcggtg atgcggtgct cagtgcgaaa ggacagtaca actggaacac cggctacggc
     4861 ggcgcggagt taggcttaag ttacccgatc accaaacatg tgcgccttta tactcaggtt
     4921 tacagcggct atggcgaatc gctcatcgac tataacttca accagacccg tgtcggtgta
     4981 ggggttatgc taaacgactt gttttgatga acggttgagt ggttggcaaa tctggaatcc
     5041 agcatccagg attaccctct cagagactaa aagcattgca gtttctcgcg caggcgctga
     5101 aaatagcgcc tgtttttatt tcaggcaatc ggggtgaatg tggcgcaggc ggaagtgttg
     5161 aatctggagt ccggagctaa acaggtttta caagaaacct ttggctacca acagtttcgc
     5221 cccggccagg aagaaattat cgacactgtg ctttccggtc gcgattgcct ggttgttatg
     5281 cccactggtg gcggaaaatc cctttgctat caaattcctg ccttattgct aaacggcctt
     5341 accgtggttg tttcaccgct gatttcgttg atgaaagatc aggtggatca actacaggcc
     5401 aacggcgtgg cggcggcgtg ccttaactcg acgcaaaccc gcgaacagca acttgaagtg
     5461 atgacaggct gccgcaccgg gcaaattcgc ttactgtata tcgccccgga acgcctgatg
     5521 ctggataact ttcttgagca tctggcgcac tggaatccgg tgttattagc cgtcgatgaa
     5581 gcgcactgta tctcccaatg gggtcacgat ttccgcccgg aatatgccgc gctcggtcag
     5641 ttgcgccagc ggttcccgac gctgccgttt atggcgctga ccgccacagc cgacgacacc
     5701 acgcgccagg atatcgtgcg cctgctgggg ctgaacgatc cgctgattca aatcagcagt
     5761 tttgaccgtc cgaatattcg ctacatgctg atggagaaat tcaaaccgct cgatcagttg
     5821 atgcgctacg tgcaggaaca gcgcggtaag tctggcatta tctactgcaa cagccgggcg
     5881 aaagtagaag acaccgctgc gcgcctgcaa agcaggggta ttagcgcggc ggcctatcat
     5941 gccgggctgg aaaataatgt ccgcgccgac gtgcaggaga aattccagcg cgatgatctg
     6001 caaattgtgg tggcgacggt ggcgttcggc atgggcatca ataaaccaaa cgttcgcttc
     6061 gtggtccact ttgatattcc gcgcaatatc gaatcctatt atcaggaaac cggtcgcgcc
     6121 gggcgtgatg gcctgcctgc ggaagcgatg ctgttttacg atccggctga tatggcgtgg
     6181 ctgcgccgtt gtctggaaga gaagccgcag gggcagttgc aggatatcga gcgccacaaa
     6241 ctcaatgcga tgggcgcgtt tgccgaagcg caaacttgcc gtcgtctggt attgctgaac
     6301 tattttggcg aagggcgtca ggagccgtgc gggaactgcg atatctgcct cgatccgccg
     6361 aaacagtacg acggttcaac cgatgctcag attgcccttt ccaccattgg tcgtgtgaat
     6421 cagcggtttg ggatgggtta tgtggtggaa gtgattcgtg gtgctaataa ccagcgtatc
     6481 cgcgactatg gtcatgacaa actgaaagtc tatggtatgg gccgtgataa aagccatgaa
     6541 cattgggtga gcgtgatccg ccagctgatt catctcggcc tggtgacgca aaatattgcc
     6601 cagcattctg ccctacaact gacagaggcc gcgcgcccgg tgctgcgcgg cgaatcctct
     6661 ttgcaacttg ccgtgccgcg tatcgtggcg ctcaaaccga aagcgatgca gaaatcgttc
     6721 ggcggcaact atgatcgcaa actgttcgcc aaattacgca aactgcgtaa atcgattgcc
     6781 gatgaaagta atgtcccgcc gtacgtggtg tttaacgacg caaccttgat tgagatggct
     6841 gaacagatgc cgatcaccgc cagcgaaatg ctcagcgtta acggcgttgg gatgcgcaag
     6901 ctggaacgct ttggtaaacc gtttatggcg ctgattcgtg cgcatgtcga tggcgacgac
     6961 gaagagtagt cagcagcata aaaaagtgcc agtatgaaga ctccgtaaac gtttcccccg
     7021 cgagtcaaat gtatgttgat gttatttctc accgtcgcca tggtgcacat tgtggcgctt
     7081 atgagccccg gtcccgattt cttttttgtc tctcagaccg ctgtcagtcg ttcccgtaaa
     7141 gaagcgatga tgggcgtgct gggcattacc tgcggcgtaa tggtttgggc tgggattgcg
     7201 ttgcttggcc tgcatttgat tatcgaaaaa atggcctggc tgcatacgct gattatggta
     7261 ggcggtggcc tgtatctctg ctggatgggt taccagatgc tacgcggtgc actgaaaaaa
     7321 gaggctgtat ctgcgcctgc accacaggtt gagctggcga aaagcgggcg cagtttcctg
     7381 aaaggcttac tgaccaatct cgccaatccg aaagcgatta tctattttgg ttcggtattc
     7441 tcattgtttg tcggcgataa cgttggcact accgagcgct ggggcatttt cgcgttgatt
     7501 attgtcgaaa cactggcgtg gtttaccgtc gttgccagtc tgtttgccct gccgcaaatg
     7561 cgccgtggtt atcaacgtct ggcgaagtgg attgatggtt ttgccggggc gttatttgcc
     7621 ggatttggca ttcatttgat tatttcgcgg tgatgccaga cgcgtcttca gagtaagtcg
     7681 gataaggcgt ttacgccgca tccgacatta tttttcacgc atgcctcgcc gatgctaaca
     7741 gcgctcccac cagcataaac aacgagccga aaatcttatt cagcgccttc atctgctttg
     7801 gtcctttaat ccatagagca atcctttgag caagggtggc gtaaccgatc atcacaataa
     7861 tatcgaccac aatagtggtg acgccgagca cgatatactg catcagttgc ggctgttgcg
     7921 gcatgatgaa ttgcggaaat agcgccgcca gaaacacaat acttttggga ttggtgagat
     7981 tcacaaaaac tgcgcgctgg aacaaatgtc gacgcgattg agtagaggcc agcgatttaa
     8041 gatcaattgc gccagcggcg cgccactgct ggattcccag ccaaatcaag taagccgcgc
     8101 ctgcccactt taacacttca aacgcaatca ctgagcggga aaatagcgtc cccaacccca
     8161 cgccaaccag cacaatatga atcgccagtc cggtctgaag cccagcaata gacgccaccg
     8221 cgccgcgata accgtggttg agcgaggtgg tcatagtgtt gattgcacca gagcctggcg
     8281 acaggcttaa aatgatcgat gtcagcaggt aggcaaacca ccattctaag gtcatgatga
     8341 actcccggtg tgtctatttt tgtgccacaa tacgctactg tcgcagcgtt gtgtcaggca
     8401 cgcttaaaaa aacgatttta cgtggtttaa gaggcagatt acccgatgtt tcagcagcaa
     8461 aaagactggg aaacaagaga aaacgcgttt gctgctttta ccatgggacc gctgactgat
     8521 ttctggcgtc agcgtgatga agcagagttt actggtgtgg atgacattcc ggtgcgcttt
     8581 gtccgttttc gcgcacagca ccatgaccgg gtggtagtca tctgcccggg gcgtattgag
     8641 agctacgtaa aatatgcgga actggcctac gacctgttcc atttggggtt tgatgtctta
     8701 atcatcgacc atcgcgggca gggacgttcc ggtcgcctgt tagccgatcc gcatctcggg
     8761 catgttaatc gctttaatga ttatgttgat gatctggcgg cattctggca gcaggaggtt
     8821 cagcccggtc cgtggcgtaa acgctatata ctggcacatt cgatgggcgg tgcgatctcc
     8881 acattatttc tgcaacgcca tccaggtgta tgtgacgcca ttgcgctaac tgcgccaatg
     8941 tttgggatcg tgattcgtat gccgtcattt atggcacggc agatcctcaa ctgggccgaa
     9001 gcgcatccac gtttccgtga tggctatgca ataggcaccg ggcgctggcg cgcgttgccg
     9061 tttgctatca acgtactgac ccacagcaga cagcgatatc gacgtaactt acgcttctat
     9121 gctgatgacc caacgattcg cgtcggtggg ccgacctacc attgggtacg cgaaagtatt
     9181 ctggctggcg aacaggtgtt agccggtgcg ggtgatgacg ccacgcaaac gcttctctta
     9241 caagctgaag aggaacgcgt ggtggataac cgcatgcacg accgtttttg tgaactccgc
     9301 accgccgcgg gccatcctgt cgaaggagga cggccgttgg taataaaagg tgcttaccat
     9361 gagatccttt ttgaaaagga cgcaatgcgc tcagtcgcgc tccacgccat cgttgatttt
     9421 ttcaacaggc ataactcacc cagcggaaac cgctctacag aggtttaaat ttcttatgta
     9481 ccaggttgtt gcgtctgatt tagatggcac gttactttct cccgaccata cgttatcccc
     9541 ttacgccaaa gaaactctga agctgctcac cgcgcgcggc atcaactttg tgtttgcgac
     9601 cggtcgtcac cacgttgatg tggggcaaat tcgcgataat ctggagatta agtcttacat
     9661 gattacctcc aatggtgcgc gcgttcacga tctggatggc aatctgattt ttgctcataa
     9721 cctggatcgc gacattgcca gcgatctgtt tggcgtagtc aacgacaatc cggacatcat
     9781 tactaacgtt tatcgcgacg acgaatggtt tatgaatcgc catcgcccgg aagagatgcg
     9841 cttttttaaa gaagcggtgt tcaaatatgc gctgtatgag ccgggattac tggagccgga
     9901 aggcatcagc aaagtgtttt tcacctgcga ttcccatgaa caactgctgc cgctggagca
     9961 ggcgattaac gctcgctggg gcgatcgcgt caacgtcagt ttctctacct taacctgtct
    10021 ggaagtgatg gcgggcggcg tgtcgaaagg ccatgcgctg gaagcagtgg cgaagaaact
    10081 gggctacagc ctgaaggatt gtattgcgtt tggtgacggg atgaacgacg ccgaaatgct
    10141 gtcgatggcg gggaaaggct gcattatggg cagtgcgcac cagcgtctga aagatctgca
    10201 tccggagctg gaagttattg gtactaatgc cgacgacgcg gtgccgcatt atctgcgtaa
    10261 actctattta tcgtaatcgt tctttatttg gtcagttgtc aacctgatac ttcgctacaa
    10321 tggatactcg ttaatcaaag agttttccat tgtggcgtta cttatcatca ccacgattct
    10381 gtgggccttc tcctttagct tttatggcga gtaccttgcg gggcacgtcg atagctattt
    10441 tgcggtgctg gtgcgcgttg gcttggcggc actcgttttt ctgccgtttc tgcgtacccg
    10501 tagcaatagc ctgaaaacgg tcggcctgta tatgctggtg ggcgcgatgc agctcggcgt
    10561 gatgtatatg ctgagtttcc gcgcttatct ctacctgacg gtctccgaat tgttgctgtt
    10621 caccgtactg acgccgctct acatcacgct gatttatgac atcatgagta agcgccgtct
    10681 gcgctggggc tatgctttta gcgccttgct ggcggtgatt ggtgccggga ttattcgcta
    10741 tgatcaggtc accgaccatt tctggactgg cttgttgctg gtgcaactct ccaatatcac
    10801 ttttgccatc ggcatggtgg gttacaaacg cctgatggaa actcgcccga tgccgcagta
    10861 taacgccttt gcgtggttct atcttggcgc gttcctggtg gcggtaattg catggttctt
    10921 gctgggaaat gcgcagaaaa tgccgcaaac cacgttgcaa tggggcattc tggtgtttct
    10981 tggcgtggtg gcttccggga ttggctactt tatgtggaac tacggcgcga cgcaggtgga
    11041 cgccggaacg ctgggcatta tgaataatat gcacgttccg gcagggctgc tggtaaacct
    11101 ggctatctgg caccaacagc cgcactggcc aacgtttatt acaggcgcgc tggtgatcct
    11161 ggcctcactg tgggtgcatc gtaagtgggt cgctccgcgc tcttcacaaa cggcagatga
    11221 tcgcaggcgt gattgcgcgc tgagcgaata aacgcttccg taactggctg acgctgctcg
    11281 ccatcgcgca cggcggcgta cagtcggctc cacaagcctt cgcccagggt tttggtcacc
    11341 accagaccct ggcgctcaaa actctctact acccaatgcg gtagcgcggc aatacccatc
    11401 cgcgcggcaa ccatttgaat caacaataag gtgttatcga cgcttttcag tgacgggctg
    11461 acgcctgccg gctgaagaaa atgccgccag acatccagtc gactacgctg caccggataa
    11521 attaatagcg tctcgctggc gagatcttcc ggtgtaatac gcgtttttgc cgccagtgga
    11581 tggtcaggtg ctaacaccag gcgtacttca taatcgaaca tcggcgaata atgcaggcca
    11641 ctgcgcggca ggatatcgga ggtcatcacc agatccagct cgccctgttg caacgcgggc
    11701 tgcgggtcaa acgtcacgcc cgatttaaaa tccatctcta cctgcggcca gttcttatgg
    11761 aaatttccta aagcgggtgt cagccactga atacagctat ggcactcaat ggcaatgcgc
    11821 agacgcgtct gctgcggttc attgcaggct tgcagtgcct ggctgatttg cggcagcacc
    11881 tggtttgcca gttgcaacag gatttctccc tgcggtgtaa agcgtagcgg ctggctctta
    11941 cgcacaaata gccggaagcc aaggcgttgt tccagatcgc taaactggtg agacagggcg
    12001 gattgcgtct gatgcaacgt tgccgcagcg gctgcgagcg agccgcagtt ccgcaacgct
    12061 tgtagcgttt tcaggtgttt tacttcgatc atgaaagtcc ttcacttcgg catgaataat
    12121 ttgcgcttga ggaatataca gtaaccgcca attatggatg tgtaaacatc tggacggcta
    12181 aaatcattcg tcttttaaat ttatggtgcg ttggctgcgt ttctccaccc cggtcactta
    12241 cttcagtaag ctcccggcga tgaataaact tgccgccttc cctaaattca aaatccatag
    12301 gatttacata taattagagg aagaaaaatg acaatattga atcacaccct cggtttccct
    12361 cgcgttggcc tgcgtcgcga gctgaaaaaa gcgcaagaaa gttattgggc ggggaactcc
    12421 acgcgtgaag aactgctgac agtagggcgt gaactgcgtg ctcgtcactg ggatcaacaa
    12481 aagcaagcgg gtatcgacct gctgccggtg ggcgattttg cctggtacga tcatgtactg
    12541 accaccagtt tgctgctggg taatgttccg ccacgtcatc agaacaaaga cggttcggta
    12601 gatatcgaca ccctgttccg tattggtcgt ggacgtgcgc cgactggcga acctgcggcg
    12661 gcagcggaaa tgaccaaatg gtttaacacc aactatcact acatggtgcc ggagttcgtt
    12721 aaaggccaac agttcaaact gacttggacg cagctgctgg aggaagtgga cgaggcgctg
    12781 gcgctgagcc acaacgttaa gcctgtgctg ctggggccgg ttacttacct gtggctgggg
    12841 aaagtgaaag gtgaacagtt tgaccgcctt agcctgctga acgacattct gccggtttat
    12901 cagcaagtgc tggcagaact ggcgaaacgc ggcatcgagt gggtacagat tgatgaaccc
    12961 gcgctggtac tggaactacc acaggcgtgg ctggacgcat acaaacccgc ttacgacgcg
    13021 ctccagggac aggtgaaact gctgctgacc acctattttg aaggcgtaac gccaaatctc
    13081 gacacgatta ctgcgctgcc tgttcagggg ctgcatgtgg accttgtaca tggtaaagat
    13141 aacgttgttg aactgcacaa gcgcctgcct tctgactggc tgctgtctgc gggtctgatc
    13201 aatggtcgta acgtctggcg cgccgatctt actgagaaat atgcgcaaat taaggacatt
    13261 gtcggcaaac gtgatttgtg ggtggcatct tcctgctcgt tgctgcacag ccccatcgac
    13321 ctgagcgtgg aaacgcgtct tgatgcagaa gtgaaaagct ggtttgcctt cgccctacaa
    13381 aaatgccatg aactggcact gctgcgcgat gcgctgaaca gtggtgacac ggcagctctg
    13441 gcagagtgga gcgccccaat tcaggcgcgt cgtcactcta ctcgtgtaca taatccggcg
    13501 gtagaaaagc gtctggcggc gatcaccgcc caggacagcc agcgtgcgaa tgtctatgaa
    13561 gtgcgtgctg aagcccagcg tgcgcgtttt aaactgccag cgtggccgac caccacgatt
    13621 ggttccttcc cgcaaaccac ggaaattcgt accctgcgtc tggatttcaa aaagggcaat
    13681 ctcgacgcca acaactaccg cacgggcatt gcggaacata tcaagcaggc cattgttgag
    13741 caggaacgtt tgggactgga tgtgctggta catggcgagg ccgagcgtaa tgacatggtg
    13801 gaatactttg gcgagcacct cgacggattt gtctttacgc aaaacggttg ggtacagagc
    13861 tacggttccc gctgcgtgaa gccaccgatt gtcattggtg acattagccg cccggcaccg
    13921 attaccgtgg agtgggcgaa gtatgcgcaa tcgctgaccg acaaaccggt gaaagggatg
    13981 ctgacggggc cggtgaccat actctgctgg tcgttcccgc gtgaagatgt cagccgtgaa
    14041 accatcgcca aacagattgc gctggcgctg cgtgatgaag tggccgatct ggaagccgct
    14101 ggaattggca tcatccagat tgacgaaccg gcgctgcgcg aaggtttacc gctgcgtcgt
    14161 agcgactggg atgcgtatct ccagtggggc gtagaggcct tccgtatcaa cgccgccgtg
    14221 gcgaaagatg acacacaaat ccacactcac atgtgttatt gcgagttcaa cgacatcatg
    14281 gattcgattg cggcgctgga cgcagacgtc atcaccatcg aaacctcgcg ttccgacatg
    14341 gagttgctgg agtcgtttga agagtttgat tatccaaatg aaatcggtcc tggcgtctat
    14401 gacattcact cgccaaacgt accgagcgtg gaatggattg aagccttgct gaagaaagcg
    14461 gcaaaacgca ttccggcaga gcgcctgtgg gtcaacccgg actgtggcct gaaaacgcgc
    14521 ggctggccag aaacccgcgc ggcactggcg aacatggtgc aggcggcgca gaatttgcgt
    14581 cgggggtaaa tccaaaccgg gtggtaatac cacccggtct tttctcatta cagcgacttc
    14641 ttcccaccat actgcttaaa ccattccagc atacgctgcc agccatcttt cgcagattcg
    14701 gcatgatagc tcgggcgata atcagcgttg aatgcatgcc cggcatccgg gtacacgata
    14761 atctctgctt tcgcattagc agcccgcagc gcctggcgca tcgtttcaac gctctcctgc
    14821 ggaatgctgt tatcctgacc accatataag ccgagaaccg gcgcgttaag atcggttgcg
    14881 atatcaacag gttgtttcgg tgaattcagc gacttgtcgc ccgtcagttt gccgtaccac
    14941 gccactgcgg cttttagctg tggattatgc gcggcataca gccaggtgat acgtccaccc
    15001 cagcagaatc cggtgatcat taaacgatga acatcgccgc cgttgcggga agcccaactg
    15061 gcgacatgat cgagatcggc cagcacctgc gagtcaggca cttttgctac cagaccgcta
    15121 agcaacgtgg ggatatcggc aaaatcattc ggatcgcctt cgcggaagta aagttcaggt
    15181 gcgatagcca gatacccctc cagcgccaga cggcgacaaa tgtcgcggat atgttcatgc
    15241 acgccaaaaa tttcctgcac tacaatgacc actggcagtg ggccatcgct ttgctttggt
    15301 ctggcatggt aagcaggcat gttatcccct tgtgaaggga tagaggtgaa gcccgccaca
    15361 atcgcgtcgt ccggggtctg aacgatggtc gaagcgagag gcgatgcagc aggtgcaaat
    15421 ccagattgtt gtgttgttgc catggtattc tccgtaccct tataaaaatg ttgcgcaatg
    15481 ttaactatag tcagcatgca acaaatcaca ttgcctgaat cggctcatct tttatgcagt
    15541 cctgcagaat gaagagtgat ttatgtgata tgaatcactt ttggtgggta aatttatgca
    15601 acgcatttgc gtcatggtga tgagtatcac gaaaaaatgt taaacccttc ggtaaagtgt
    15661 ctttttgctt cttctgacta aaccgattca cagaggagtt gtatatgtcc aagtctgatg
    15721 tttttcatct cggcctcact aaaaacgatt tacaaggggc tacgcttgcc atcgtccctg
    15781 gcgacccgga tcgtgtggaa aagatcgccg cgctgatgga taagccggtt aagctggcat
    15841 ctcaccgcga attcactacc tggcgtgcag agctggatgg taaacctgtt atcgtctgct
    15901 ctaccggtat cggcggcccg tctacctcta ttgctgttga agagctggca cagctgggca
    15961 ttcgcacctt cctgcgtatc ggtacaacgg gcgctattca gccgcatatt aatgtgggtg
    16021 atgtcctggt taccacggcg tctgtccgtc tggatggcgc gagcctgcac ttcgcaccgc
    16081 tggaattccc ggctgtcgct gatttcgaat gtacgactgc gctggttgaa gctgcgaaat
    16141 ctattggcgc gacaactcac gttggcgtga cagtttcttc tgataccttc tacccaggtc
    16201 aggaacgtta cgatacttac tctggtcgcg tagttcgtca ctttaaaggt tctatggaag
    16261 agtggcaggc gatgggcgta atgaactatg aaatggaatc tgcaactctg ctgaccatgt
    16321 gtgcaagtca gggcctgcgt gccggtatgg tagcgggtgt tatcgttaac cgcacccagc
    16381 aagagatccc gaatgctgag acgatgaaac aaaccgaaag ccatgcggtg aaaatcgtgg
    16441 tggaagcggc gcgtcgtctg ctgtaattct cttctcctgt ctgaaggccg acgcgttcgg
    16501 ccttttgtat ttttgcgtag cgcctcgcag gaaatgcctt tccaactgga cgtttgtaca
    16561 gcacaattct attttgtgcg ggtaagttgt tgcgtcagga ggcgttgtgg atttctcaat
    16621 catggtttac gcagttattg cgttggtggg tgtggcaatt ggctggctgt ttgccagtta
    16681 tcaacatgcg cagcaaaaag ccgagcaatt agctgaacgt gaagagatgg tcgcggagtt
    16741 aagcgcggca aaacaacaaa ttacccaaag cgagcactgg cgtgcagagt gtgaattgct
    16801 caataacgaa gtgcgcagcc tgcaaagtat taacacctct ctggaggccg atctgcgtga
    16861 agtaaccacg cggatggaag ccgcacagca acatgctgac gataaaattc gccagatgat
    16921 taacagcgag cagcgcctca gtgagcagtt tgaaaacctc gccaaccgta tttttgagca
    16981 cagcaatcgc cgggttgatg agcaaaaccg tcagagtctg aacagcctgt tgtcgccgct
    17041 acgtgaacaa ctggacggtt tccgccgtca ggttcaggac agcttcggta aagaagcaca
    17101 agaacgccat accctgaccc acgaaattcg caatctccag caactcaacg cgcaaatggc
    17161 ccaggaagcg atcaacctga cgcgcgcgct gaaaggcgac aataaaaccc agggcaactg
    17221 gggcgaggta gtattgacgc gggtgctgga ggcttccggt ctgcgtgaag ggtatgaata
    17281 tgaaacccag gtcagcatcg aaaatgacgc ccgctcgcgg atgcagccgg atgtcatcgt
    17341 gcggctgccg cagggaaaag atgtggtgat cgacgccaaa atgacgctgg tcgcctatga
    17401 acgctatttt aacgccgaag acgactacac ccgcgaaagc gcgctacagg aacatatcgc
    17461 gtcggtgcgt aaccatatcc gtttgctggg acgcaaagat tatcaacagc tgccggggct
    17521 gcgaactctg gattacgtgc tgatgtttat tcccgttgaa cccgcttttt tactggcgct
    17581 tgaccgccag ccggagctga tcaccgaagc gttgaaaaac aatatcatgc tggttagccc
    17641 gactacgctg ctggtggcgt tgcgtactat tgccaacctg tggcgttatg agcatcaaag
    17701 ccgtaacgcc cagcaaatcg ccgatcgtgc cagcaagctg tacgacaaga tgcgtttgtt
    17761 catcgatgac atgtccgcga ttggtcaaag tctcgacaaa gcgcaggata actaccgcca
    17821 ggcaatgaaa aaactctctt caggtcgcgg aaatgtgctg gcgcaggcag aagcgtttcg
    17881 cggtttagga gtagaaatta aacgcgagat taatccggat ttggctgaac aggcggtgag
    17941 ccaggatgaa gagtatcgac ttcggtcggt tccggagcag ccgaatgatg aagcttatca
    18001 acgcgatgat gaatataatc agcagtcgcg ctagcccatt aggggtagtt aagccgggta
    18061 gaaatctagg gcatcgacgc ccaatctgtt acacttctgg aacaattttt tgatgagcag
    18121 gcattgagat ggtggataag tcacaagaaa cgacgcactt tggttttcag accgtcgcga
    18181 aggaacaaaa agcggatatg gtcgcccacg ttttccattc cgtggcatca aaatacgatg
    18241 tcatgaatga tttgatgtca tttggtattc atcgtttgtg gaagcgattc acgattgatt
    18301 gcagcggcgt acgccgtggg cagaccgtgc tggatctggc tggtggcacc ggcgacctga
    18361 cagcgaaatt ctcccgcctg gtcggagaaa ctggcaaagt ggtccttgct gatatcaatg
    18421 aatccatgct caaaatggga cgcgagaagc tgcgtaatat cggtgtgatt ggcaacgttg
    18481 agtatgttca ggcgaacgct gaggcgctgc cgttcccgga taacaccttt gattgcatca
    18541 ccatttcgtt tggtctgcgt aacgtcaccg acaaagataa agcactgcgt tcaatgtatc
    18601 gcgtgctgaa acccggcggc cgcctgctgg tgcttgagtt ctcgaagcca attatcgagc
    18661 cgctgagcaa agcctatgat gcatactcct tccatgtgct gccgcgtatt ggctcactgg
    18721 tcgcgaacga cgccgacagc taccgttatc tggcagaatc catccgtatg catcccgatc
    18781 aggataccct gaaagccatg atgcaggatg ccggattcga aagtgtcgac tactacaatc
    18841 tgacggcagg ggttgtggcg ctgcatcgtg gttataagtt ctgacaggag accggaaatg
    18901 ccttttaaac ctttagtgac ggcaggaatt gaaagtctgc tcaacacctt cctgtatcgc
    18961 tcacccgcgc tgaaaacagc ccgctcgcgt ctgctgggta aagtattgcg cgtggaggta
    19021 aaaggctttt cgacgtcatt gattctggtg ttcagcgaac gccaggttga tgtactgggc
    19081 gaatgggcag gcgatgctga ctgcaccgtt atcgcctacg ccagtgtgtt gccgaaactt
    19141 cgcgatcgcc agcagcttac cgcactgatt cgcagtggtg agctggaagt gcagggcgat
    19201 attcaggtgg tgcaaaactt cgttgcgctg gcagatctgg cagagttcga ccctgcggaa
    19261 ctgctggccc cttataccgg tgatatcgcc gctgaaggaa tcagcaaagc catgcgcgga
    19321 ggcgcaaagt tcctgcatca cggcattaag cgccagcaac gttatgtggc ggaagccatt
    19381 actgaagagt ggcgtatggc acccggtccg cttgaagtgg cctggtttgc ggaagagacg
    19441 gctgccgtcg agcgtgctgt tgatgccctg accaaacggc tggaaaaact ggaggctaaa
    19501 tgacgccagg tgaagtacgg cgcctatatt tcatcattcg cactttttta agctacggac
    19561 ttgatgaact gatccccaaa atgcgtatca ccctgccgct acggctatgg cgatactcat
    19621 tattctggat gccaaatcgg cataaagaca aacttttagg tgagcgacta cgactggccc
    19681 tgcaagaact ggggccggtt tggatcaagt tcgggcaaat gttatcaacc cgccgcgatc
    19741 tttttccacc gcatattgcc gatcagctgg cgttattgca ggacaaagtt gctccgtttg
    19801 atggcaagct ggcgaagcag cagattgaag ctgcaatggg cggcttgccg gtagaagcgt
    19861 ggtttgacga ttttgaaatc aagccgctgg cttctgcttc tatcgcccag gttcataccg
    19921 cgcgattgaa atcgaatggt aaagaggtgg tgattaaagt catccgcccg gatattttgc
    19981 cggtcattaa agcggatctg aaacttatct accgtctggc tcgctgggtg ccgcgtttgc
    20041 tgccggatgg tcgccgtctg cgcccaaccg aagtggtgcg cgagtacgaa aagaccttga
    20101 ttgatgaact gaatttgctg cgggaatctg ccaacgccat tcagcttcgg cgcaattttg
    20161 aagacagccc gatgctctac atcccggaag tttaccctga ctattgtagt gaagggatga
    20221 tggtgatgga gcgcatttac ggcattccgg tgtctgatgt tgcggcgctg gagaaaaacg
    20281 gcactaacat gaaattgctg gcggaacgcg gcgtgcaggt gttcttcact caggtctttc
    20341 gcgacagctt tttccatgcc gatatgcacc ctggcaacat cttcgtaagc tatgaacacc
    20401 cggaaaaccc gaaatatatc ggcattgatt gcgggattgt tggctcgcta aacaaagaag
    20461 ataaacgcta tctggcagaa aactttatcg ccttctttaa tcgcgactat cgcaaagtgg
    20521 cagagctaca cgtcgattct ggctgggtgc caccagatac caacgttgaa gagttcgaat
    20581 ttgccattcg tacggtctgt gaacctatct ttgagaaacc gctggccgaa atttcgtttg
    20641 gacatgtact gttaaatctg tttaatacgg cgcgtcgctt caatatggaa gtgcagccgc
    20701 aactggtgtt actccagaaa accctgctct atgtcgaagg ggtaggacgc cagctttatc
    20761 cgcagctcga tttatggaaa acagcgaagc ctttcctgga gtcgtggatt aaagatcagg
    20821 tcggtattcc tgctctggtg agagcattta aagaaaaagc gccgttctgg gtcgaaaaaa
    20881 tgccagaact gcctgaactg gtttacgaca gtttgcgcca gggcaagtat ttacagcaca
    20941 gtgttgataa gattgcccgc gagcttcagt caaatcatgt acgtcaggga caatcgcgtt
    21001 attttctcgg aattggcgct acgttagtat taagtggcac attcttgttg gtcagccgac
    21061 ctgagtgggg gctgatgccc ggctggttaa tggcaggtgg tctgatcgcc tggtttgtcg
    21121 gttggcgcaa aacacgctga ttttttcatc gctcaaggcg ggccgtgtaa cgtataatgc
    21181 ggctttgttt aatcatcatc taccacagag gaacatgtat gggtggtatc agtatttggc
    21241 agttattgat tattgccgtc atcgttgtac tgctttttgg caccaaaaag ctcggctcca
    21301 tcggttccga tcttggtgcg tcgatcaaag gctttaaaaa agcaatgagc gatgatgaac
    21361 caaagcagga taaaaccagt caggatgctg attttactgc gaaaactatc gccgataagc
    21421 aggcggatac gaatcaggaa caggctaaaa cagaagacgc gaagcgccac gataaagagc
    21481 aggtgtaatc cgtgtttgat atcggtttta gcgaactgct attggtgttc atcatcggcc
    21541 tcgtcgttct ggggccgcaa cgactgcctg tggcggtaaa aacggtagcg ggctggattc
    21601 gcgcgttgcg ttcactggcg acaacggtgc agaacgaact gacccaggag ttaaaactcc
    21661 aggagtttca ggacagtctg aaaaaggttg aaaaggcgag cctcactaac ctgacgcccg
    21721 aactgaaagc gtcgatggat gaactacgcc aggccgcgga gtcgatgaag cgttcctacg
    21781 ttgcaaacga tcctgaaaag gcgagcgatg aagcgcacac catccataac ccggtggtga
    21841 aagataatga agctgcgcat gagggcgtaa cgcctgccgc tgcacaaacg caggccagtt
    21901 cgccggaaca gaagccagaa accacgccag agccggtggt aaaacctgct gcggacgctg
    21961 aaccgaaaac cgctgcacct tccccttcgt cgagtgataa accgtaaaca tgtctgtaga
    22021 agatactcaa ccgcttatca cgcatctgat tgagctgcgt aagcgtctgc tgaactgcat
    22081 tatcgcggtg atcgtgatat tcctgtgtct ggtctatttc gccaatgaca tctatcactt
    22141 ggtatccgcg ccattgatca agcagttgcc gcaaggttca acgatgatcg ccaccgacgt
    22201 ggcctcgccg ttctttacgc cgatcaagct gacctttatg gtgtcgctga ttctgtcagc
    22261 gccggtgatt ctctatcagg tgtgggcatt tatcgcccca gcgctgtata agcatgaacg
    22321 tcgcctggtg gtgccgctgc tggtttccag ctctctgctg ttttatatcg gcatggcatt
    22381 cgcctacttt gtggtctttc cgctggcatt tggcttcctt gccaataccg cgccggaagg
    22441 ggtgcaggta tccaccgaca tcgccagcta tttaagcttc gttatggcgc tgtttatggc
    22501 gtttggtgtc tcctttgaag tgccggtggc aattgtgctg ctgtgctgga tggggattac
    22561 ctcgtcagaa gacttacgca aaaaacgccc gtatgtgctg gttggtgcat tcgttgtcgg
    22621 gatgttgctg acgccgccgg atgtcttctc gcaaacgcta ttggcgatcc cgatgtactg
    22681 tctgtttgaa atcggtgtct tcttctcacg cttttacgtt ggtaaagggc gaaatcggga
    22741 agaggaaaac gacgctgaag cagaaagcga aaaagctgaa gcagaaagcg aaaaaactga
    22801 agaataaatt caaccgcccg tcagggcggt tgtcatatgg agtacaggat gtttgatatc
    22861 ggcgttaatt tgaccagttc gcaatttgcg aaagaccgtg atgatgttgt agcgcgcgct
    22921 tttgacgcgg gagttaatgg gctactcatc accggcacta acctgcgtga aagccagcag
    22981 gcgcaaaagc tggcgcgtca gtattcgtcc tgttggtcaa cggcgggcgt acatcctcac
    23041 gacagcagcc agtggcaagc tgcgactgaa gaagcgatta ttgagctggc cgcgcagcca
    23101 gaagtggtgg cgattggtga atgtggtctc gactttaacc gtaacttttc gacgccggaa
    23161 gagcaggaac gcgcttttgt tgctcagcta cgcattgccg cagaattaaa catgccggta
    23221 tttatgcact gtcgcgatgc ccacgagcgg tttatgacat tgctggagcc gtggctggat
    23281 aaactgcctg gtgcggttct tcattgcttt accggcacac gcgaagagat gcaggcgtgc
    23341 gtggcgcgtg gaatttatat cggcattacc ggttgggttt gcgatgaacg acgcgggctg
    23401 gagctgcggg aattgttgcc gttgattccg gcggagaaat tgctgatcga aacagatgcg
    23461 ccgtatctgc tcccgcgcga tctcacgcca aagccatcat cccggcgcaa cgagccagcc
    23521 catctgcccc atattttgca acgtattgcg cactggcgtg gagaagatgc cgcatggctg
    23581 gctgccacca cggatgccaa tgtcaaaaca ctgtttggga ttgcgtttta gagtttgcgg
    23641 aactcggtgt tcttcacact gtgcttaatc tctttattaa taagattaag caatagcatg
    23701 gagcgagcct caccatcggg ttcggtgaaa atggcctgaa agccttcgaa cgcgccttcg
    23761 gtaataatca ccttatcacc cggataaggg ggggccggat cgacaatgtc tttcggttta
    23821 tataccgata gctgatgaat taccgccgat gggactatcg ctggcgacgc gccaaagcgc
    23881 acaaagtggc tgacgccacg cgtagcgctg atggtcgtgg tatgaatcac ttctggatca
    23941 aattccacaa acaggtagtt agggaataac ggttcactga ctgcggtacg tttaccccgt
    24001 acgatttttt ccagggtgat catcggagcg aggcaattaa ccgcctgtct ctcaaggtgt
    24061 tcctgggcac gttgaagttg cccgcgcttg cagtacagta aataccagga ttgcataatg
    24121 actcttatcc gtttaatcgg ggcgcaagga tagcaaaagc tttacgctaa gttaattata
    24181 ttccccggtt tgcgttatac cgtcagagtt cacgctaatt taacaaattt acagcattgc
    24241 aaagatgaac gccgtataat gggcgcagat taagaggcta caatggacgc catgaaatat
    24301 aacgatttac gcgacttcct gacgctgctt gaacagcagg gtgagctaaa acgtatcacg
    24361 ctcccggtgg atccgcatct ggaaatcact gaaattgctg accgcactct gcgtgctggt
    24421 gggcctgcgc tgttgttcga aaaccctaaa ggctactcaa tgccggtgct gtgcaacctg
    24481 ttcggtacgc caaagcgcgt ggcgatgggc atggggcagg aagatgtttc ggcgctgcgt
    24541 gaagttggta aattattggc gtttctgaaa gagccggagc cgccaaaagg tttccgcgac
    24601 ctgtttgata aactgccgca gtttaagcag gtgttaaaca tgccgacaaa gcgactgcgt
    24661 ggtgcgccct gccaacaaaa aatcgtctct ggcgatgacg tcgatctcaa tcgcattccc
    24721 attatgacct gctggccgga agatgccgcg ccgctgatta cctgggggct gaccgtaacg
    24781 cgcggcccgc ataaagagcg gcagaatctg ggcatttatc gccagcagct gattggtaaa
    24841 aacaaactga ttatgcgctg gctgtcgcat cgcggcggcg cgctggatta tcaggagtgg
    24901 tgtgcggcgc atccgggcga acgtttcccg gtttctgtgg cgctgggtgc cgatcctgcc
    24961 acgattctcg gtgcagtcac ccccgttccg gatacgcttt cagagtatgc gtttgccgga
    25021 ttgctacgcg gcaccaaaac cgaagtagta aagtgtattt ccaatgacct cgaagtgcca
    25081 gccagtgccg aaatcgtcct ggaagggtat atcgatcctg gtgagatggc gccggaaggg
    25141 ccgtatggtg accacacagg ttactataat gaagtcgata atttcccggt gtttaccgtg
    25201 acgcatatta cccagcgtga agatgcgatt taccattcca cctataccgg gcgtccgcca
    25261 gatgagcccg cggtactggg cgtggcgttg aacgaagtgt ttgtaccgat tctgcaaaaa
    25321 cagttcccgg aaattgtcga tttttacctg ccgccggaag gctgttctta tcgtctggcg
    25381 gtagtgacga tcaaaaaaca gtacgccgga cacgcgaagc gcgtcatgat gggcgtctgg
    25441 tcgttcttac gccagtttat gtacactaaa tttgtgatcg tttgcgatga tgacgtcaac
    25501 gcacgcgact ggaacgatgt gatttgggcg attaccaccc gtatggaccc ggcgcgggat
    25561 actgttctgg tagaaaatac gcctattgat tatctggatt ttgcctcgcc tgtctctggg
    25621 ctgggttcaa aaatggggct ggatgccacg aataaatggc cgggggaaac ccagcgtgaa
    25681 tggggacgtc ccatcaaaaa agatccagat gttgtcgcgc atattgacgc catctgggat
    25741 gaactggcta tttttaacaa cggtaaaagc gcctgatgcg cgtttgtttt gccctattta
    25801 tcgatccgac agagaaagcg catgacaacc ttaagctgta aagtgacctc ggtagcagct
    25861 atcacggata ccgtatatcg tgtccgcatc gtgccagacg cggccttttc ttttcgtgct
    25921 ggtcagtatt tgatggtagt gatggatgag cgcgacaaac gtccgttctc aatggcctcg
    25981 acgccggatg aaaaagggtt tatcgagctg catattggcg cttctgaaat caacctttac
    26041 gcgaaagcag tcatggaccg catcctcaaa gatcatcaaa tcgtggtcga ccttccccac
    26101 ggagaagcgt ggctgcgcga tgatgaagag cgtccgatga ttttgattgc gggcggcacc
    26161 gggttctctt atgcccgctc gattttgctg acagcgttgg cgcgtaaccc aaaccgtgat
    26221 atcaccattt actggggcgg gcgtgaagag cagcatctgt atgatctctg cgagcttgag
    26281 gcgctttcgt taaagcacct tggtctgcaa gtggtgccgg tggttgaaca accggaagca
    26341 ggctggcgtg ggcgtactgg taccgtgtta acggcggtat tgcaggatca tggtacgctg
    26401 gcagagcatg atatctatat tgccggacgt tttgagatgg cgaaaattgc ccgtgacctg
    26461 ttttgcagtg agcgtaatgc gcgggaagat cgcctgtttg gcgatgcgtt tgcatttatc
    26521 tgagatataa aaaaacccgc ccctgacagg cgggaagaac ggcaactaaa actttattca
    26581 gtggcattta gatctatgtc gcatccggca attggtgcac gatgcctgat gcgacgctgg
    26641 cgcgtcttat caggcctaca ttggtgccgg atcggtagac cggataaggc gttcacgccg
    26701 catccggcaa gtggttaaac ccgctcaaac accgtcgcaa taccctgacc cagaccgata
    26761 cacatcgtcg ccagaccaaa ctgaacgtct ttgcgttcca tcagattcag cagcgtggtg
    26821 ctgatacgcg caccggaaca acccagcgga tgacccagcg cgatcgcacc accattgagg
    26881 ttgatcttct cgtcaatctg ctccattagc ccgagatctt taatgcacgg caggatctgc
    26941 gcggcaaagg cttcgttcat ctcaaacacg ccgatatcgc tggcagaaag ccccgctttt
    27001 ttcagcgcca gcttcgaggc cggaaccggg ccgtaaccca taatcgatgg gtcacaacca
    27061 acgaccgcca tcgaacgcac acgagcgcgc ggcttaagac ctaattcatg ggcgcggctt
    27121 tcactcatca ccagcatggc agctgcgcca tcggaaagtg cagaagatgt gcccgccgtt
    27181 accgtaccgt ttactggatc aaacgccgga cgcagcgtgg cgagggcttc cacggtggtt
    27241 tccgggcgaa tcacttcgtc gtaattaaac tgcttcagga cgccgtcggc atcgtgacca
    27301 ccagttggga tgatttcatt tttaaatgcg gccgactgcg tggcggccca ggcgcgggcg
    27361 tgtgaccgcg cggcaaaggc atcctgcatt tcacggctga taccgtgcat acgcgccagc
    27421 atttctgccg ttaagcccat catgcccgcc gctttggcga cattgcggct caggccgggg
    27481 tgaaaatcga cgccgtgact catcggcaca tggcctatat gctccacgcc gccaaccaga
    27541 catgcctgcg tatcgccagt catgatcatt cgtgctgcgt catgcagtgc ctgcatggat
    27601 gaaccacaca agcgattgac ggtaaccgcc gggacagagt gcggcacttc tgccagcagc
    27661 gccgcgttac gggcgatgtt aaaaccctgc tccagcgtct gttgcacaca accccagtaa
    27721 atatcgtcga gggccgccgc ttccagcgcc gggttacgcg ccagcaggct acgcattaaa
    27781 tgagcggaga gatcttccgc acgcacgtta cgaaaagcac cgcccttcga acggcccatc
    27841 ggggtgcgaa ttgcatcgac aatgacaacc tgttccattg tgactcctta agccgttttc
    27901 aggtcgccaa ccggacgggc tggctcaacc ggaggatagt acggttcgtt atgacgcgct
    27961 ttattacgca gaccttccgg cacttcatac agcgggccga ggtgctgata ttgctgtgcc
    28021 atatcgaggt attttgcgct accgagggtg tccagccagc ggaacgcgcc gccgtggaac
    28081 ggagggaagc ccaggccgta gaccagcgcc atatccgctt ccgccggagt ggcgataatg
    28141 ccctcttcca gacagcgcac cacttcgttg accatcggga tcatcatgcg ggcgataatc
    28201 tcttcttcgc tgaaatcgcg cttcggttgg ctcacttctg ccagcaggtc ttcaacggcg
    28261 gcgtcttctt ctttcttcgg cttacctttg ctgtcttctt tataacgcca gaaaccgagg
    28321 ccgttcttct gaccaaagcg gttggcatca aacagcgcgt cgatggcatc gcggtaatct
    28381 ttctgcatcc gctgcgggaa gcctgctgcc atgacagcct gagcgtgatg cgcggtatca
    28441 atgcccacaa cgtccagcag atatgccggg cccatcggcc agccaaactg tttttccatc
    28501 actttgtcga tcttgcggaa atccgcgccg tcgcgcagca gctggctgaa accggcgaaa
    28561 tacgggaaca gcacgcggtt aacaaagaag ccggggcagt cgttaaccac aatcggcgtc
    28621 ttgcccatct tgctcgccca ggcgacaact ttcgcgatgg tttcgtcgga gcttttctcg
    28681 ccgcgaataa tttctaccaa cggcattcgg tggaccgggt taaagaagtg catcccgcag
    28741 aagttttccg ggcgttccag cgcgttggcc agttcgctga taggaatggt tgaagtgtta
    28801 gacgccagca cggtatcctg gcgtactttt tgttcggttt ctgccagtac ggcttttttc
    28861 actttcgggt tttcaacaac cgcttctacc acaatatcca cgcggtcaaa tccggcgtag
    28921 tcgagcgttg ggtggatcgt ggagatcacg ccagccagtt tcagaccatc gattttgccg
    28981 cgctcaagct gcttgttcag cagtttcgcg gcttcggtca tgccgagggt taacgacttg
    29041 tcgttgatat ctttcatgac aaccggcacg cctttccacg cggactggta agcgataccg
    29101 ccacccatga tgcctgcgcc cagcacagca gcctgtttcg gggtttcaac gtctttggtg
    29161 agtttcttcg ctttgccttt tacatattga tcgttaagga aaatgccgac cagtgcgcga
    29221 gcttcgttgg tatgcgccag cgggacaaaa cttttgtttt ccagatttaa ggcttcttca
    29281 cgaccaaaac gggccgcagc ttcaatggtt tttactgcgg tgatgggggc cggataatgt
    29341 ttccccgccg tttgcgcgac cattccttta gcgatggtga agctcatggt ggcttcaatc
    29401 ttgctcagtt ttagcggttc ctgcttcggc tgacgtttcg ctttccagtc gaggtcgccg
    29461 ttaatggcct ggcgtaaaat cgccatcgcc ccttcaatca gtttttctgc tttgactacg
    29521 ccatccacca gaccgatttt cagcgcctga tccgcgccga catctttacc ggcggcaatg
    29581 atttccagtg cactgtcagc gcccagcata cgtggcatac gtacagaacc gccaaagccc
    29641 ggcatgatgc ccagtttggt ttccggcaga ccgatgcgca gatccggcgt cgccagacga
    29701 taatcggtcg ccagcacgca ttcgcagcca ccacccagcg catagccgtt gacggcagca
    29761 atggtcggca ccggcagatc ttccaggcga ttaaacacgc tattggcaaa gtgcagccac
    29821 tgacttaact gttcttcagg aacgaggaac agggacaaaa attcggtgat atcagcaccg
    29881 acgataaagg ctgctttgtt cgaacgcagc agcagccctt ttagatctga ttgctgttcc
    29941 agcacgccga tggcctcgcc gaggctggcg acggtcgcag tgtcgagttt attaactgaa
    30001 cctggggcat caaataccag ttcggcaatg ccatcttcca gccagtcaag gtacagggtg
    30061 tcgcctttgt aaagcatgtc agtctcctga atccgcaagg taatctggtc gtaccagatg
    30121 catcggagtg tgtgttttgt gttaaaaata tgcaaataaa agattaaaga aatgccgatc
    30181 tgatcacgct cggcagaaat cacgctctgg atgaacgatg tgctaagatg cggagactta
    30241 aggtcaaaaa aacagaaggg taaaaaatgg aatcactggc ctcgctctat aaaaatcata
    30301 tagctacctt acaagaacgg actcgcgatg cgctggcgcg cttcaagctg gatgcattac
    30361 ttattcactc cggcgagctg ttcaacgttt ttctcgacga tcatccctat ccgtttaaag
    30421 tgaacccgca attcaaagcg tgggtgccgg taactcaggt gccaaactgc tggttgctgg
    30481 tggatggcgt gaacaagccg aaactgtggt tctatctgcc ggttgattac tggcacaacg
    30541 tcgaaccgct gccgacctcc ttctggactg aagatgtgga agtgatcgcg ctgccgaaag
    30601 ccgatggcat tggtagcctg ctgcctgctg cgcgcggcaa tatcggttat atcggtccgg
    30661 tgccggaacg tgcgctgcaa ctgggtattg aggccagcaa catcaacccg aaaggggtga
    30721 tcgactacct gcattattac cgctccttca aaaccgagta cgaactggcc tgtatgcgtg
    30781 aagcgcagaa aatggcggtc aatggtcatc gtgcggcaga agaagcgttc cgttctggca
    30841 tgagcgagtt cgatatcaat attgcctatc tgaccgcgac cggtcatcgt gataccgacg
    30901 taccttacag caacattgtg gcgcttaacg aacacgctgc ggtgttgcat tacaccaaac
    30961 tggaccacca ggcaccggaa gagatgcgca gcttcctgct ggatgccggg gccgaatata
    31021 acggctatgc ggctgacctg acccgtacct ggtcggcaaa aagcgacaac gactacgcac
    31081 agctggtgaa agacgtaaat gatgaacaac tggcgctgat cgccaccatg aaagcaggcg
    31141 tcagctatgt ggattaccac atccagttcc atcagcgcat cgccaaactg ctgcgtaaac
    31201 atcaaatcat caccgatatg agtgaagaag cgatggtcga aaacgatctc accggaccgt
    31261 ttatgccgca cggtattggc catccgctgg gcctgcaggt gcatgacgtc gccggtttta
    31321 tgcaggatga tagcggtaca cacctcgcgg caccggcaaa atatccgtac ctgcgctaca
    31381 cccgtattct ccagccgggc atggtgttaa ccatcgaacc gggtatctac ttcattgaat
    31441 cgcttctggc accgtggcgt gaagggcagt tcagcaagca cttcaactgg cagaaaattg
    31501 aagcactgaa accgttcggc ggcattcgta tcgaagacaa cgtggtgatc cacgaaaaca
    31561 acgtggaaaa catgacccgg gatctgaaac tggcgtgatg gaaagctggt taattcctgc
    31621 ggcaccggtc acggtcgttg aagagatcaa aaagagccgc ttcataacga tgttggcgca
    31681 taccgatggc gttgaggcgg cgaaagcgtt tgttgaatcg gtgcgggcag aacaccccga
    31741 tgcccgccac cattgcgtgg cgtgggtggc gggtgcgccg gatgattctc aacagctggg
    31801 tttctctgac gacggggagc cggcgggaac ggcaggtaaa ccgatgctcg cccagctaat
    31861 gggcagcggc gtcggggaaa ttaccgctgt ggtagtgcgc tactacggcg gcatattgct
    31921 tggcaccggt gggttagtga aagcgtatgg cggcggcgtg aatcaggcgc tgcgccagct
    31981 aacgacccaa cgcaagacgc cattaaccga atatactttg caatgtgaat atagtcagtt
    32041 aaccggcatt gaagcgttgc tggggcagtg tgacggcaaa attatcaaca gtgattatca
    32101 ggcattcgtt ctgctgcggg tggcgcttcc ggcggcgaaa gtggctgaat tttccgcaaa
    32161 gctggcggat tttagccgtg gttcattgca attgttagcg attgaagaat aatccccact
    32221 tcgttttgca gaactaagga agcggcagag atgcattttc gcgccattac ccgaatcgtt
    32281 ggactactgg tcatcttatt ttcagggacc atgattatcc ctgggctggt agcactcatc
    32341 taccgggatg gagcgggccg cgcttttacc cagacctttt ttgtcgccct cgccattggc
    32401 tctatgctgt ggtggccgaa ccgcaaagag aagggcgaac tgaaatcccg tgaggggttt
    32461 ctgatagtgg tgctgttttg gaccgtgctg ggtagtgtcg gtgcgctccc ttttatcttc
    32521 tcggaaagcc cgaacctcac gattaccgat gcgttttttg aatctttctc tggcctgacc
    32581 accaccgggg ccactacgct ggtggggctg gattcgctcc ctcatgccat cctcttttat
    32641 cgccagatgc tgcaatggtt tggcgggatg gggatcatcg tgttagcggt tgcgatactg
    32701 cctatcctcg gcgtgggtgg gatgcagctc tatcgcgcag aaatgcccgg cccgctgaaa
    32761 gataacaaaa tgcgcccgcg aattgcggaa acggcgaaaa ctctgtggtt gatttatgtc
    32821 ttgctgaccg tcgcctgtgc gctggcgttg tggtttgccg gaatggatgc ctttgatgcc
    32881 atcggccata gctttgcgac tatcgctatt ggcggcttct cgacacatga tgccagtatc
    32941 ggttatttcg acagtccgac tattaacact atcattgcta tcttcctgct gatctccggc
    33001 tgtaactacg gtctgcactt ttcactgtta agtgggcgta gtctgaaggt ttattggcgc
    33061 gatccggaat ttcgcatgtt tatcggcgta cagtttacgc tggtggttat ttgtacactc
    33121 gtactgtggt ttcataatgt ctacagttcg gcgctgatga caattaacca ggcgtttttc
    33181 caggtggtat cgatggcgac aaccgccggg tttacgactg acagcattgc ccgctggccg
    33241 ctctttttgc cggtactgct tttatgttca gcttttatcg gcggttgcgc cgggtcaacg
    33301 ggcggtggcc tgaaagtgat ccgcatcctg ctgctgttta agcaggggaa ccgtgagctg
    33361 aaacgactgg tgcatccgaa cgccgtctat agcattaagc tggggaatcg cgcactgccg
    33421 gaacgtatcc tcgaagccgt ttggggattt ttctccgcct atgcattggt gtttattgtc
    33481 agtatgctgg cgattatcgc cacgggcgtg gatgattttt ctgcctttgc ctcagttgtt
    33541 gcgacattga ataacctggg gccagggctt ggcgtggttg ctgataactt taccagtatg
    33601 aacccggtgg ctaaatggat cctgattgcc aacatgctgt ttggtcgtct cgaggtcttt
    33661 acattactgg tgctctttac cccgactttc tggcgtgaat gatggagtaa tacgtgaaaa
    33721 cattaattct tttctcaaca agggacggac aaacgcgcga gattgcctcc tacctggctt
    33781 cggaactgaa agaactgggg atccagacgg atgtcgccaa tgtgcaccgc attgaagaac
    33841 cacaatggga aaactatgac cgtgtggtca ttggtgcttc tattcgctat ggtcactacc
    33901 actctgcgtt ccaggaattt gtcaaaaaac atgcgacgcg actgaattcg atgccgagcg
    33961 ccttttattc cgtaaacctg gtggcgcgca aaccggagaa gcgtactcca cagaccaaca
    34021 gctacgcgcg gaagtttctg atgaactcgc aatggcgtcc cgatcgctgc gcggtcattg
    34081 ccggggcgct gcgttaccca cgttatcgct ggtacgaccg ttttatgctc aagctgatta
    34141 tgaagatgtc aggcggtgaa acggatacgc gcaaagaagt tgtctatacc gattgggagc
    34201 aggtggcgaa tttcgcccga gaaatcgccc atttaaccga caaaccgacg ctgaaataag
    34261 cataaagaat aaaaaatgcg cggtcagaaa attattttaa atttcctctt gtcaggccgg
    34321 aataactccc tataatgcgc caccactgac acggaacaac ggcaaacacg cagccgggtc
    34381 agcggggttc tcctgagaat tccgacagag aaagcaaaaa taaatgcttg actttgtagc
    34441 gggaaagcgt attatgaaca ccccgcgc
//
LOCUS       DAJTQQ010000037        32690 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000037 DAJTQQ010000000
VERSION     DAJTQQ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.037"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(2..94)
                     /locus_tag="OO309_001947"
     tRNA            complement(2..94)
                     /locus_tag="OO309_001947"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO309_001948"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO309_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR5847102.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO309_001949"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO309_001949"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR5847103.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO309_001950"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO309_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR5847104.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO309_001951"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO309_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR5847105.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO309_001952"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO309_001952"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR5847106.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO309_001953"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO309_001953"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR5847107.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="OO309_001954"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="OO309_001954"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="OO309_001955"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="OO309_001955"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR5847108.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="OO309_001956"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="OO309_001956"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR5847109.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="OO309_001957"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="OO309_001957"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR5847110.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="OO309_001958"
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="OO309_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR5847111.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="OO309_001959"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="OO309_001959"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR5847112.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="OO309_001960"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="OO309_001960"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR5847113.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO309_001961"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO309_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR5847114.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="OO309_001962"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="OO309_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR5847115.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="OO309_001963"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="OO309_001963"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR5847116.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO309_001964"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO309_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR5847117.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO309_001965"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO309_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR5847118.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO309_001966"
     CDS             complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO309_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR5847119.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20900..21019)
                     /locus_tag="OO309_001967"
     CDS             complement(20900..21019)
                     /locus_tag="OO309_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HCR5847120.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO309_001968"
     CDS             complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO309_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR5847121.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO309_001969"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO309_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR5847122.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..25511)
                     /gene="hycC"
                     /locus_tag="OO309_001970"
     CDS             complement(23682..25511)
                     /gene="hycC"
                     /locus_tag="OO309_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005092744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR5847123.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALRRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGIILLGLGLGLGLGLGLGLGLGLGAGVTGIALEQPALIALGLVGGLYHLLNHS
                     LFKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGE
                     WVIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAE
                     NATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLL
                     IACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLK
                     LRKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(25548..26159)
                     /gene="hycB"
                     /locus_tag="OO309_001971"
     CDS             complement(25548..26159)
                     /gene="hycB"
                     /locus_tag="OO309_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR5847124.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(26284..26745)
                     /gene="hycA"
                     /locus_tag="OO309_001972"
     CDS             complement(26284..26745)
                     /gene="hycA"
                     /locus_tag="OO309_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR5847125.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            26957..27307
                     /gene="hypA"
                     /locus_tag="OO309_001973"
     CDS             26957..27307
                     /gene="hypA"
                     /locus_tag="OO309_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5847126.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            27311..28183
                     /gene="hypB"
                     /locus_tag="OO309_001974"
     CDS             27311..28183
                     /gene="hypB"
                     /locus_tag="OO309_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR5847127.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            28174..28446
                     /gene="hypC"
                     /locus_tag="OO309_001975"
     CDS             28174..28446
                     /gene="hypC"
                     /locus_tag="OO309_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR5847128.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            28446..29567
                     /gene="hypD"
                     /locus_tag="OO309_001976"
     CDS             28446..29567
                     /gene="hypD"
                     /locus_tag="OO309_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR5847129.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            29564..30574
                     /gene="hypE"
                     /locus_tag="OO309_001977"
     CDS             29564..30574
                     /gene="hypE"
                     /locus_tag="OO309_001977"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR5847130.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            30648..>32690
                     /gene="flhA"
                     /locus_tag="OO309_001978"
     CDS             30648..>32690
                     /gene="flhA"
                     /locus_tag="OO309_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR5847131.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRM"
BASE COUNT         7704 a         9124 c         8525 g         7337 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggccggcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctggcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgcctg gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtgga ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagattgttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgggcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactag catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat accgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacactcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 aggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctaccggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    23881 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    23941 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    24001 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    24061 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    24121 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    24181 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    24241 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    24301 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    24361 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    24421 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    24481 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    24541 accaggccaa gagcaatcag cgccggttgt tcgagcgcga tacccgttac gccagcgccc
    24601 agccccagcc ccagccccag ccccagcccc agccccagcc ccagccccag ccccagcagg
    24661 ataatgccga tattttccag ggtgtggtaa gccagcaggc gctggatgtt gtgttccatc
    24721 aacgcataca gaccaccgac gaacgcggtg atcatgccga gcaccagcag cgcgatcccc
    24781 caccacagcg gtgcattacc gcccagcagt gacagggtta acatgcccag caggccaatt
    24841 ttcatgacta ccgtagaaaa caacgcggca gctggcgcag aggcgttagc atgtgcctgc
    24901 ggcacccagc cgtgcagcgg aataatccca gccagcaggc caaagccaat cacaccgagc
    24961 agccagatat cggaaccgag cggcagctgt tgcatacgca tatccagcag gcgcagatcc
    25021 agcgtgccat aacgctgcca cagcagccag caagcaatca ccagcagcag agtgccaaga
    25081 cgcctcagcg caaaccacag tttgccctct ttgctgttgc tggtgaggaa caccgcacac
    25141 aaggccatga cttcggccat taccacgaac atgccgaggt tgctggcgat gacggcgcag
    25201 acggcggcag ccatcaacat attgatctgc aagccgttgc acttcacctg cgcgtggcga
    25261 tgccagtcaa tgttatagag gctgacaaac agaccgcaaa ggccgagggt aatcagccaa
    25321 atcgcgttaa gcggggagat ttgcacatcg tagcttacca gcgacagcgc accgctcacg
    25381 ccaaccgcgc cagtcagtac agtgaagccc gcggctgccg tatacagact accaaccgcg
    25441 ccgccaattc cggctatcca gccacttagc gctttttgaa aagagaaatg ccagaacagc
    25501 ggcggcgaca aaccatgcca cgccgctatt gatcagggaa attgcgctca tttagcctct
    25561 ccactttgag cctgctgaaa caaggtgaga tcgccaaagt ccgtgttaaa ggtcagctca
    25621 cgcttacgtt tgctgacgcg ggcgatatcg gtgttatcca ccagatgcag ggctttagtc
    25681 gggcacatcc gcacacaggc cggaccttgt tcatcaaagc tacaaaggtc acatttgacg
    25741 gcgatcgcgc gaatacctgg cacccagtca agcaatgtgc tgacacgcgc cggagcaggc
    25801 ggtgccggtg gcgctttcgg ggtattggcg tttgccggaa tatccagcgg acggctgccg
    25861 gaaaattcaa ttgcgccaaa cgggcaggcg atgccgcaca gtttgcagct tacgcacaga
    25921 ctttcattca actgcacggc cccatcaacg cgggtgatgg cgttaaccgg gcagaccacc
    25981 gcgcagggtg catcttcaca gtggtgacag agctgcggcg cagattcttt ttcattgagc
    26041 atcactctca ggcgcggcat tgattgcagg ccgtgctggc gatgcgtctc tgaacaggcg
    26101 gcctcacagg tgtggcagcc gatacagagc gtggagtcgg caattacaaa acgattcacc
    26161 aggcattcct caggtgattg tcatttttga cgaaaacatg ccgatgaaat gtcattttcg
    26221 acactcatcg acacgcccat ccccaaacag gcgtaacgcc tgcaaaacgg gcaaagcctc
    26281 agctcatgct gccgggcttt gtccctttac cagttggctt aaattcaccg gcacattgtt
    26341 ttcaatggcg gtgtataagc tgtcgtaaac gccagcgatt ttttcgagat agtgttccag
    26401 aacctgttcg cgatcgcgga tgttgacctt gccatcaatc atgccgtcaa tgctcagttg
    26461 cgcctgcgca atccgctgtt tgtcttcgcc atcttttgtt tcgacgtaat actcgatggt
    26521 gtggctgtta aagccatccg ccagggtgac gtaaatacga aaatgttcaa aaaggacgaa
    26581 acagagttct ttgtccggcg cgcagctcat ggcgtgatgc aggcgcgctt tcgataacgt
    26641 gatcccctga accagcgaat tgcagtagat gtgccactgg tcctgtaggc gacgatgccg
    26701 ctgtgcgatg taatcggctt tctcgcttat ttcccaaata gtcattgtca ggttactcgt
    26761 ttaacagaga tgccagcttt aagcattttt tgtgccaact tttaatttat tgttattaaa
    26821 gagattttta agctaaagat gaatttcgtc gccgtgtcga cgtgtcattt cgacatcatc
    26881 gacattattc accgcaggga taatcaacac tggcgcaatt attgcttgta gctggcaata
    26941 gttaatggga ggcgatatgc acgaaataac cctctgccaa cgggcactgg aattgatcga
    27001 acagcaggcc gcaaaacacg gcgcaaaacg cgtaactggg gtctggctca aaattggcgc
    27061 attttcttgt gtcgaaacca gctctcttgc cttttgtttt gatctggttt gccgcggcag
    27121 cgtggcggaa ggttgtaaac tgcacctcga agaacaagag gccgaatgct ggtgtgaaac
    27181 atgccaacag tatgtgacgc tactgaccca gcgcgtccgc cgctgtccac agtgtcatgg
    27241 tgacatgcta cagattgtgg cagacgacgg tttacagatt cggcggatag aaatagacca
    27301 ggagtgagcg atgtgtacaa catgcggttg cggtgaaggc aacctgtata tcgagggtga
    27361 tgaacataac cctcattccg cgtttcgtag cgcgccattt gccccggcgg cacgcccgca
    27421 gatgaaaatc accggcatta aagcgcctga atttaccccc agccagactg aagaaggcga
    27481 cctgcattac ggtcatggcg aagcgggcac tcacacaccg ggcatgagcc agcgtcggat
    27541 gctggaagtc gaaattgacg tgctggacaa aaacaaccgt ctggctgaac gcaaccgcgc
    27601 gcgctttgct gcccgcaagc aactggtgct caacctggtt tccagccctg gttccggtaa
    27661 aaccaccctg ctgacggaaa ccttaatgcg cctgaaagac agcgttccgt gcgcggttat
    27721 tgaaggcgac cagcaaaccg tgaacgatgc cgcacgcatt cgcgctaccg gtacaccagc
    27781 gattcaggtg aacaccggta aaggctgcca tcttgacgca cagatgattg ccgacgccgc
    27841 accgcgtctg ccactggacg ataacggtat tctgtttatc gaaaacgttg gcaacctcgt
    27901 atgcccggcc agcttcgatc tcggtgaaaa acacaaagta gcggtgcttt ccgttaccga
    27961 aggtgaagac aaaccgctga aatatccgca tatgtttgcc gccgcctcgc tgatgctgct
    28021 caacaaagtt gacctgttgc cgtatctcaa ctttgacgtt gagaagtgca tcgcctgcgc
    28081 ccgcgaagtc aatccagaaa ttgaaatcat ccttatttcc gccaccagcg gcgaagggat
    28141 ggaccagtgg ctgaactggc tggagacaca gcgatgtgca taggcgttcc cggccagatc
    28201 cgcaccattg acggcaacca ggcgaaagtc gacgtctgcg gcattcagcg cgatgtcgat
    28261 ttaacgttag tcggcagctg cgatgaaaac ggtcagccgc gcgtgggcca gtgggtactg
    28321 gttcacgttg gctttgccat gagcgtaatt aatgaagccg aagcacgcga cactctcgac
    28381 gccttacaaa acatgtttga cgttgagccg gatgtcggcg cgctgttgta tggcgaggaa
    28441 aaataatgcg ttttgttgat gaatatcgcg cgccggaaca ggtgatgcag ttaattgagc
    28501 atctgcgcga acgtgcttca catctctctt acaccgccga acgccctctg cggattatgg
    28561 aagtgtgtgg cggtcatacc cacgccattt ttaaattcgg cctcgaccag ttactgcctg
    28621 aaaacgttga gtttatccac ggtccgggtt gcccggtgtg cgtactgccg atgggtagaa
    28681 tcgacacctg cgtggagatt gccagccatc cggaagtcat cttctgtacc tttggcgacg
    28741 ccatgcgcgt accagggaaa cagggatcgc tgttgcaggc aaaagcacgc ggtgccgatg
    28801 tgcgcatcgt ttactcgccg atggatgcgt tgaaactggc gcaggagaat ccaacccgca
    28861 aagtggtgtt cttcggctta ggttttgaaa ccaccatgcc gaccaccgcc atcactctgc
    28921 aacaggcgaa agcgcgtgat gtgcagaatt tttacttctt ctgccagcac attacgctta
    28981 tcccgacgct gcgcagtttg ctggaacagc cggataacgg tatcgacgcg ttcctcgcgc
    29041 cgggccacgt tagtatggtt atcggcactg atgcctataa ttttatcgcc agcgattttc
    29101 atcgtccgct ggtggtcgct ggtttcgaac cccttgatct actgcaaggc gtggtcatgc
    29161 tggtggagca gaaaatagcg gcccacagca aggtagagaa tcagtatcgt cgggtggtac
    29221 cggatgccgg taacctactg gcgcaacagg cgattgccga tgtgttctgt gtcaacggcg
    29281 acagcgaatg gcgcggctta ggcgtgattg aatcttctgg cgtgcacctg acgccggatt
    29341 atcaacgatt cgatgccgaa gcacatttcc gcccggcacc gcagcaggtc tgcgatgacc
    29401 cgcgcgcgcg ttgtggtgag gtattaacgg gcaaatgtaa gccgcatcaa tgcccgctgt
    29461 ttggtaacac ctgtaatcct caaaccgcat ttggtgcact gatggtctcc tccgaaggag
    29521 cgtgcgccgc gtggtatcag tatcgtcagc aggagagtga agcgtgaata atatccaact
    29581 cgcccacggc agcggcggcc aggcgatgca gcaattaatc aacagcctgt ttatggaagc
    29641 ctttgccaac ccgtggctgg cagagcagga agatcaggca cgtcttgatc tggcgcagct
    29701 ggtagcggaa ggcgaccgtc tggcgttctc caccgacagt tacgtcattg acccgctgtt
    29761 ctttcctggc ggtaatatcg gcaagctggc gatttgcggc accgcgaatg acgttgcggg
    29821 cagtggcgct attccgcgct atctctcctg tggctttatc ctcgaagaag gattgccgat
    29881 ggagacactg aaagccgtag tgaccagcat ggcagaaacc gcccgcacgg caggcattgc
    29941 catcgttact ggcgatacta aagtggtgca gcgcggcgcg gcagataaac tgtttatcaa
    30001 caccgctggc atgggcgcaa ttccggcgaa tattcactgg ggcgcacaga cgctaaccgc
    30061 aggcgatgta ttgctggtta gcggtacact cggcgaccac ggggcgacta tccttaacct
    30121 gcgtgagcag ctggggctgg atggcgaact ggtcagcgac tgcgcggtgc tgacgccgct
    30181 tattcagacg ctgcgtgaca ttcccggcgt gaaagcgctg cgtgatgcca cccgtggtgg
    30241 tgtaaacgcg gtggttcatg agttcgcggc agcctgcggt tgtggtattg aaatttctga
    30301 atcagcgctg ccggttaaac ctgccgtgcg cggcgtttgc gaattgctgg gactggacgc
    30361 cctgaacttt gccaacgaag gcaaactggt gatcgccgtt gaacgcaacg cggcagagca
    30421 agtgctggca gcgttacatt cccatccact ggggaaagac gcggcgctga ttggtgaagt
    30481 ggtggaacgt aaaggtgttc gtcttgccgg tctgtatggc gtgaaacgaa ccctcgattt
    30541 accacacgcc gaaccgcttc cgcgtatatg ctaataaaat tctaaatctc ctatagttag
    30601 tcaatgacct tttgcaccgc tttgcggtgc tttcctggaa gaacaaaatg tcatatacac
    30661 cgatgagtga tctcggacag caagggttgt tcgacatcac tcggacacta ttgcagcagc
    30721 ccgatctggc ctcgctgtgt gaggctcttt cgcaactggt aaagcgttct gcgctcgccg
    30781 acaacgcggc tattgtgttg tggcaagcgc agactcaacg tgcgtcttat tacgcgtcgc
    30841 gtgaaaaaga cacccccatt aaatatgaag acgaaactgt tctggcacac ggtccggtac
    30901 gcagcatttt gtcgcgccct gatacgctgc attgcagtta cgaagaattt tgtgaaacct
    30961 ggccgcagct ggccgcaggt gggctatacc ccaaatttgg tcactattgc ctgatgccac
    31021 tggcggcggg agggcatatt tttggtggct gtgaatttat tcgttatgac gagcgtccct
    31081 ggagcgaaaa agagttcaat cgtctgcaaa catttacgca gatcgtttct gtcgtcaccg
    31141 aacaaatcca gagccgcgtc gtcaacaatg tcgactatga gttgttatgc cgggagcgcg
    31201 ataacttccg catcctggtt gccattacca acgcggtgct ttcccgcctg gatatggacg
    31261 aactggtcag cgaagtcgcc aaagaaatcc attactattt cgacattgac gatatcagca
    31321 ttgtcttacg cagccaccgt aaaaacaaac tcaacatcta ctccactcac tatcttgata
    31381 aacaacatcc cgcccacgaa cagagcgaag tcgatgaagc cggaaccctc accgaacgcg
    31441 tgttcaaaag taaagagatg ctgctgatca atctctacga gcgggacgat ttagccccct
    31501 atgaacgcat gttgttcgac acctggggca accagattca aaccttgtgc ctgttaccgc
    31561 tgatgtctgg cgacaccatg ctgggcgtgc tgaaactggc gcaatgcgaa gagaaagtgt
    31621 ttaccactac caatctgaat ttgctgcgcc agattgccga acgtgtggca atcgctgtcg
    31681 ataacgccct cgcctatcag gaaatccatc gtctgaaaga acggctggtt gatgaaaacc
    31741 tcgccctgac cgagcagctc aacaatgttg atagtgaatt tggcgagatt attggccgca
    31801 gcgaagccat gtacagcgtg cttaaacaag ttgaaatggt ggcgcaaagt gacagtaccg
    31861 tgctgatcct cggtgaaact ggctcgggta aagagctgat tgcccgtgcg atccataatc
    31921 tcagtgggcg taataatcgc cgcatggtca aaatgaactg cgcggcgatg cctgccggat
    31981 tgctggaaag cgatctgttt ggtcatgagc gtggggcttt taccggtgcc aacgcccagc
    32041 gcattggtcg ttttgaactg gcggataaaa gctctctgtt cctcgacgaa gtgggcgata
    32101 tgccactgga gttacagccg aagttgctgc gtgtattgca ggaacaggag tttgaacgtc
    32161 tcggcagcaa caaaatcatt cagacggacg tacgtttaat tgccgcgact aaccgcgatc
    32221 tgaaaaaaat ggtcgccgac cgagagttcc gtagcgatct ctattaccgc ctgaacgtat
    32281 tcccgattca cctgccgcca ctacgcgagc gtccggaaga tattccgctg ctggcgaaag
    32341 cctttacctt caaaattgcc cgtcgtctgg ggcgcaatat cgacagcatt cctgccgaga
    32401 cgctgcgcac cttgagcaac atggagtggc cgggtaacgt acgcgaactg gaaaacgtca
    32461 ttgagcgcgc agtattgcta acacgcggca acgtgctgca gctgtcattg ccagatattg
    32521 ctttaccgga gcctgaaacg ccgcctgccg caacggttgt cgcccaggag ggcgaagatg
    32581 aatatcagtt gattgtgcgc gtgctgaaag aaaccaacag cgtggttgcc gggcctaaag
    32641 gcgctgcgca acgtctgggg ctgaaacgca cgaccctgct gtcacggatg
//
LOCUS       DAJTQQ010000038        31438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000038 DAJTQQ010000000
VERSION     DAJTQQ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.038"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            77..1354
                     /gene="waaA"
                     /locus_tag="OO309_001979"
     CDS             77..1354
                     /gene="waaA"
                     /locus_tag="OO309_001979"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR5847132.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1362..1841
                     /gene="coaD"
                     /locus_tag="OO309_001980"
     CDS             1362..1841
                     /gene="coaD"
                     /locus_tag="OO309_001980"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR5847133.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1880..2689)
                     /gene="mutM"
                     /locus_tag="OO309_001981"
     CDS             complement(1880..2689)
                     /gene="mutM"
                     /locus_tag="OO309_001981"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR5847134.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2787..2954)
                     /gene="rpmG"
                     /locus_tag="OO309_001982"
     CDS             complement(2787..2954)
                     /gene="rpmG"
                     /locus_tag="OO309_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR5847135.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2975..3211)
                     /gene="rpmB"
                     /locus_tag="OO309_001983"
     CDS             complement(2975..3211)
                     /gene="rpmB"
                     /locus_tag="OO309_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR5847136.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3428..4096)
                     /gene="radC"
                     /locus_tag="OO309_001984"
     CDS             complement(3428..4096)
                     /gene="radC"
                     /locus_tag="OO309_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR5847137.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4268..5488
                     /gene="coaBC"
                     /locus_tag="OO309_001985"
     CDS             4268..5488
                     /gene="coaBC"
                     /locus_tag="OO309_001985"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR5847138.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5466..5924
                     /gene="dut"
                     /locus_tag="OO309_001986"
     CDS             5466..5924
                     /gene="dut"
                     /locus_tag="OO309_001986"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR5847139.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6031..6627
                     /gene="slmA"
                     /locus_tag="OO309_001987"
     CDS             6031..6627
                     /gene="slmA"
                     /locus_tag="OO309_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR5847140.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6664..7305)
                     /gene="pyrE"
                     /locus_tag="OO309_001988"
     CDS             complement(6664..7305)
                     /gene="pyrE"
                     /locus_tag="OO309_001988"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR5847141.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7371..8087)
                     /gene="rph"
                     /locus_tag="OO309_001989"
     CDS             complement(7371..8087)
                     /gene="rph"
                     /locus_tag="OO309_001989"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR5847142.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8214..9077
                     /locus_tag="OO309_001990"
     CDS             8214..9077
                     /locus_tag="OO309_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR5847143.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9298..10122
                     /gene="dinD"
                     /locus_tag="OO309_001991"
     CDS             9298..10122
                     /gene="dinD"
                     /locus_tag="OO309_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR5847144.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10413..11030
                     /locus_tag="OO309_001992"
     CDS             10413..11030
                     /locus_tag="OO309_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR5847145.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11027..12709)
                     /gene="ligB"
                     /locus_tag="OO309_001993"
     CDS             complement(11027..12709)
                     /gene="ligB"
                     /locus_tag="OO309_001993"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR5847146.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12967..13590
                     /gene="gmk"
                     /locus_tag="OO309_001994"
     CDS             12967..13590
                     /gene="gmk"
                     /locus_tag="OO309_001994"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR5847147.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13645..13920
                     /gene="rpoZ"
                     /locus_tag="OO309_001995"
     CDS             13645..13920
                     /gene="rpoZ"
                     /locus_tag="OO309_001995"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR5847148.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13939..16047
                     /gene="spoT"
                     /locus_tag="OO309_001996"
     CDS             13939..16047
                     /gene="spoT"
                     /locus_tag="OO309_001996"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR5847149.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16054..16743
                     /gene="trmH"
                     /locus_tag="OO309_001997"
     CDS             16054..16743
                     /gene="trmH"
                     /locus_tag="OO309_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR5847150.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16749..18830
                     /gene="recG"
                     /locus_tag="OO309_001998"
     CDS             16749..18830
                     /gene="recG"
                     /locus_tag="OO309_001998"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR5847151.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18864..20069)
                     /gene="gltS"
                     /locus_tag="OO309_001999"
     CDS             complement(18864..20069)
                     /gene="gltS"
                     /locus_tag="OO309_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR5847152.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20349..21740
                     /gene="xanP"
                     /locus_tag="OO309_002000"
     CDS             20349..21740
                     /gene="xanP"
                     /locus_tag="OO309_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR5847153.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21861..23570
                     /locus_tag="OO309_002001"
     CDS             21861..23570
                     /locus_tag="OO309_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5847154.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23623..25941)
                     /gene="yicI"
                     /locus_tag="OO309_002002"
     CDS             complement(23623..25941)
                     /gene="yicI"
                     /locus_tag="OO309_002002"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR5847155.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25951..27334)
                     /locus_tag="OO309_002003"
                     /pseudo
     CDS             complement(25951..27334)
                     /locus_tag="OO309_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27627..27721
                     /locus_tag="OO309_002004"
     tRNA            27627..27721
                     /locus_tag="OO309_002004"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27661..27663,aa:Sec,seq:tca)
     gene            27993..29204
                     /locus_tag="OO309_002005"
     CDS             27993..29204
                     /locus_tag="OO309_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5847156.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            29616..30659
                     /locus_tag="OO309_002006"
     CDS             29616..30659
                     /locus_tag="OO309_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR5847157.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31174..>31438
                     /locus_tag="OO309_002007"
     CDS             31174..>31438
                     /locus_tag="OO309_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5847158.1"
                     /translation="MDENALRFASYWRNSLADAESGKGCFERKDAQNFTHWHGIAAGR
                     LDEAIVNKFFKGEKDDVETVNVILRPKVYFRLLQHGKDRSAGTP"
BASE COUNT         7880 a         8287 c         7800 g         7471 t
ORIGIN      
        1 aaaacgggaa aagtaatggt aaagccacag ctaaatacat agaatcccca gcacatccat
       61 aagtcagcta tttactatgc tcgaattgct ttacaccgcc cttctctacc ttattcagcc
      121 gctgatctgg atacggctct gggtgcgcgg acgtaaggct ccggcctatc gaaaacgctg
      181 gggtgaacgt tacggttttt accgccatcc gctaaaacca ggcggcatta tgctgcactc
      241 cgtctccgtc ggtgaaactc tggcggcgat cccgttggtg cgcgcgctgc gtcatcgtta
      301 tcctgattta ccgattaccg ttacaaccat gacgccaacc ggttcggagc gcgtacaatc
      361 ggctttcggg aaggatgttc agcacgttta tctgccgtat gacctgcccg atgcgctcaa
      421 ccgtttcctg aataaagtcg accccaaact ggtgttgatt atggaaaccg aactatggcc
      481 taacctgatt gcggcgctac ataaacgtaa aattccgctg gtgatcgcta acgcgcgact
      541 ctctgcccgc tcggccgcag gttatgccaa actgggtaaa ttcgtccgcc gcttgctgcg
      601 tcgtattacg ctgattgctg cgcaaaatga agaagatggt gcacgttttg tggcgctggg
      661 cgcaaaaaat aaccaggtaa ccgttaccgg tagcctgaaa ttcgatattt ctgtaactcc
      721 gcagttggct gctaaagccg tgacgctacg ccgccaatgg gcaccacacc gcccggtatg
      781 gattgccacc agcactcacg aaggtgaaga gagcgtggtc attgccgcac atcaggcatt
      841 gttacagcaa ttcccgaatt tattgctcat cctggtaccc cgtcatccag aacgcttccc
      901 ggatgcgatt aaccttgtcc gccaggctgg actaagctat atcacacgct cttcagggga
      961 agtcccctcc accagcacgc aggttgtggt tggcgatacg atgggcgagt tgatgttact
     1021 gtacggcatt gccgatctcg cctttgttgg cggttcactg gttgaacgtg gtgggcataa
     1081 tccgctggaa gctgccgcac acgctattcc ggtattgatg gggccgcata cttttaactt
     1141 taaagacatt tgcgcgcggc tggagcaggc aagcgggctt attaccgtta ccgatgccac
     1201 tacgcttgca aaagaggttt cctctttact caccgacgcc gtttaccgta gtttctatgg
     1261 ccgtcatgcc gttgaagtac tgtatcaaaa ccagggcgcg ctacagcgtc tgcttcaact
     1321 gctggaacct tacctgccac cgaaaacgca ttgaggttgt tatgcaaaaa cgggcgattt
     1381 atccgggtac tttcgatccc attaccaatg gtcatatcga tatcgtgacg cgcgccacgc
     1441 agatgttcga tcacgttatt ctggcgattg ccgccagccc cagtaaaaaa ccgatgttta
     1501 ccctggaaga gcgtgtggca ctggcacagc aggcaaccgc gcatctgggg aacgtggaag
     1561 tggtcgggtt tagtgatttg atggcgaact tcgcccgtaa tcaacacgct acggtgctga
     1621 ttcgtggcct gcgtgcggtg gcggattttg aatatgaaat gcagctggcg catatgaatc
     1681 gccacttaat gccggaactg gaaagtgtgt ttctgatgcc gtcgaaagag tggtcgttta
     1741 tctcttcatc gttggtgaaa gaggtggcgc gccatcaggg cgatgtcacc catttcctgc
     1801 cggagaatgt ccatcaggcg ctgatggcaa aattagcgta gcgtttatgc cggatggtat
     1861 gccatccggc tcgcatgaat tacttctggc actgccgaca ataaaacgtt gcccgctgcg
     1921 catgtttagt cgccacaatc ggcgtaccgc acacccgaca cggctcacct tttcgcccgt
     1981 aaacctgcaa ttcctgcgcg aaatagcccg gtttaccatc actttgcaga aaatctttca
     2041 gcgttgtacc accctgctca atcgaacgca gcaacaccgc tttaatcacc cgagctaaca
     2101 attcacactc tgccagcgac agtgatgacg ccagccgatc cggatggatc cccgccgcaa
     2161 acagtgattc gctggcatag atattcccta cccctaccac cagcttgtta tccatcagcc
     2221 acggtttaat cgccgttttt ttcttcgcgc acttctgatg cagatactca ccattgaaat
     2281 cgtcgctaag cggctccggt ccaagatggg ccagcacatt atgcccttcc agctctttgg
     2341 tccacagcca ggcaccaaag cggcgcggat cggtgtagcg cagcacttta ccgttgctca
     2401 tcaccaaatc cacatggtca tgcttttcag ggggaagttc ttctggaagg atgcgcaggc
     2461 tgccagacat ccccaaatga atgataatcc agccctcagg cagctccagc agcagatatt
     2521 tagcccgccg ctgcacgcta agcactggtt gatcgcttaa tcggtagatc tcttctgaaa
     2581 ccggccagcg caagcgtccg ttgcgcacca ccgcatgaag aatggttgca ccaacgagat
     2641 gcggttctat gccgcgacgg ctggtttcaa cttcgggtaa ttcaggcata gcatctccag
     2701 gaatgaacag atgcaatcaa tatgggggca agcagataac aaaaaacccc gccggagcga
     2761 ggtttttcgt tacatcaaag cgagaattat ttgattttcg cttctttgta gatcacgtgc
     2821 tgacgaacaa ctggatcgaa ttttttcagt tccagttttt ccggcttagt acgtttgttc
     2881 ttcgtagtgg tatagaagtg accagtacca gcagaagaaa ccagcttgat tttctcacga
     2941 atacctttag ccatgattta tttcctctaa gtacttagta cttttcgcca cgggcacgca
     3001 gttcagccag aactgtatcg atgccttttt tatcgattac acgcatacct ttagcagata
     3061 cgcgcagggt gacaaaacgc ttctcgctct caacccagaa acggtgagag tgcaggttcg
     3121 gcaggaaacg gcgtttagtc gcgttcagtg cgtgggaacg gttgttaccg gtcaccggac
     3181 gcttgccagt aacttggcag actcgggaca tgtctattct ccaaaaatca aattagctcg
     3241 agcttcgtat ggggtattgg cgcctcgtca ggctttacag cccggtcatc gcagttctat
     3301 gtgaactctc gattgccagg cccaaatgcc aaacccgaga ttctcaaagg tggcgtagta
     3361 tacgctgact cagcgatgtg ctcaagtccc gaacagacaa agatcccgaa ggatcgcgca
     3421 tagcgggtta aatccagccg cgttcggcaa aagaaacata ctctccacgc ccaatcacga
     3481 tatggtcgag cacgcgtaaa tccatgaact gacaactctt tattatccgt tcagtaatga
     3541 gtttatccgc tttactgggt tcagcacaac ccgaagggtg attatgtgca aggatcagcg
     3601 ccgaggcgtt tatttttatc gcttcgcgga taatttcccg aggatggact tcaacatggt
     3661 ttagcgtgcc ggaaaaaaga cggctatgcg ttataacccg gtgttgtgag tcgagaaaga
     3721 tcaccataaa gatctcccgc tcctcacccg tgagctggct ttgtaaaaat tcccgcgtca
     3781 tctccgggct gagtaaaggg ctttcttcac gcatccgcac gttgtagtaa cgtcgcgcca
     3841 gttcagcaat cccctttaac tgggcaaatt tcgctacgcc aattccatga acaccactaa
     3901 attgttcata ttcagaggtt aacaagccat aaagagagcc gaaattctcc agcatctctt
     3961 ttgccagggt taatacatct ttaccgcgcg ttccggtacg cagaaatagc gccagcagct
     4021 cgacatccgt taaggcgcta ataccaaact tcagcatttt ttcgcgcggc atcaacagct
     4081 gtgcattgtt tttcaccttc acctcctttg tggtgctcgc atcctgtcat agccgttccg
     4141 ttaaatcgac ggccagtttt caatcctgga aagagcctcg caaagtgaat ctcaggtgaa
     4201 ggcacgacaa caaaagtatt gtgataaaat cgccaacttc tggtgtcacc caacaggaaa
     4261 aatcatcatg agcctggccg gtaaaaaaat cgttctcggc gttagcggcg gtattgctgc
     4321 ctataaaacc cctgaactgg tgcgtcgttt gcgcgatcgc ggggccgacg tccgcgtagc
     4381 catgaccgaa gcggcaaaag cctttatcac cccacttagc ttgcaggcgg tttctggtta
     4441 tcccgtttcc gacagtctgc ttgacccggc agccgaagcc gctatgggcc atattgagct
     4501 gggtaaatgg gctgatttag tgattctcgc ccctgccacg gcagatttga ttgcccgtgt
     4561 tgctgccgga atggcgaatg acctggtatc gacgatttgt ctggctacac ctgcgcctgt
     4621 agccgtgctc cccgccatga accagcagat gtaccgtgcc gctgccacgc agcataattt
     4681 agaggtgctt gcttcccgtg gtttgctcat ctgggggcca gacagtggca gtcaggcttg
     4741 tggtgatatc ggtcctggac gaatgctcga tccgttaacc attgtggata tggcggtggc
     4801 gcatttttcg cccgtcaacg acctgaaaca tctgaacatt atgattaccg ccggtccgac
     4861 gcgtgaaccg ctcgatccgg tgcgttatat ctctaatcac agctccggca agatgggttt
     4921 tgctatcgcc gccgctgctg cccgtcgtgg cgcgaacgtc acgctggtat caggtccagt
     4981 ttcactaccg acgccaccgt ttgttaaacg tgttgatgtg atgaccgcgc tggaaatgga
     5041 agccgccgtg aatgcttctg tacagcagca aaatattttt atcggttgcg ccgccgtggc
     5101 ggattatcgc gcagctaccg tggccccaga aaaaatcaaa aagcaggcca cgcagggtga
     5161 tgaattaaca ataaaaatgg ttaaaaaccc cgatatcgtc gcaggcgttg ccgcactaaa
     5221 agaccatcga ccctacgtcg ttggatttgc cgccgaaaca aataatgtgg aagaatacgc
     5281 ccggcaaaaa cgtatccgta aaaaccttga tctgatctgc gcgaacgatg tttcccagcc
     5341 aactcaagga tttaacagcg acaacaacgc attacacctt ttctggcagg acggagataa
     5401 agtcttaccg cttgagcgca aagagctcct tggccaatta ttactcgacg agatcgtgac
     5461 ccgttatgat gaaaaaaatc gacgttaaga ttctggaccc gcgcgttagg aaggaatttc
     5521 cgctcccgac ttatgccacc tctggctctg ccggacttga cctgcgtgcc tgtctcgacg
     5581 acgccgtaga actggctccg ggtgacacta cgctggttcc gaccgggctg gcgattcata
     5641 ttgccgatcc ttcactggcg gcaatgatgc tgccgcgctc cggattggga cataagcacg
     5701 gtatcgtgct tggtaacctg gtaggattga tcgattccga ctatcagggc cagttgatga
     5761 tttccgtgtg gaaccgtggt caggacagct tcaccattca acctggcgaa cgcatcgccc
     5821 agatgatttt tgttccggta gtacaggctg aatttaatct ggtggaagat ttcgacgcca
     5881 ccgaccgcgg tgaaggcggc tttggtcact ctggtcgtca gtaacacata cgcatccgaa
     5941 taacgtcata acatagccgc aaacatttcg tttgcggtca tagcgtgggt gccgcctggc
     6001 aagtgcttat tttcaggggt attttgtaac atggcagaaa aacaaactgc gaaaaggaac
     6061 cgtcgcgagg aaatacttca gtctctggcg ctgatgctgg aatccagcga tggaagccaa
     6121 cgtatcacga cggcaaaact ggccgcctct gtcggcgttt ccgaagcggc actgtatcgc
     6181 cacttcccca gtaagacccg catgttcgat agcctgattg agtttatcga agatagcctg
     6241 attactcgca tcaacctgat tctgaaagat gagaaagaca ccacagcgcg cctgcgtctg
     6301 attgtgttgc tgcttctcgg ttttggtgag cgtaatcctg gcctgacccg catcctcact
     6361 ggtcatgcgc taatgtttga acaggatcgc ctgcaagggc gcatcaacca gctgttcgag
     6421 cgtattgaag cgcagctgcg ccaggtattg cgtgaaaaga gaatgcgtga gggtgaaggt
     6481 tacaccaccg atgaaaccct gctggcaagc cagatcctgg ccttctgtga aggtatgctg
     6541 tcacgttttg tccgcagcga atttaaatac cgcccgacgg atgattttga cgcccgctgg
     6601 ccgctaattg ccgcgcagtt gcagtaatat gacgccggat gacttttcat ccggcgagtt
     6661 tctttaaacg ccaaactctt cgcgataggc cttaaccgcc gccagatgtt ccgccatttc
     6721 cggcttctct tccagatagg caatcagatc tttcagggtg atgatagaga tcactttgca
     6781 gttgtaatca cgctcaactt cctgaatcgc cgaaatctcg ccgcgcccgc gttcctgacg
     6841 atcgagcgaa atcaacacgc cagcaagcgt cgcgccattg gcctgaataa tctccatcga
     6901 ctcgcgaatc gccgttccgg cggtgatcac atcatctacc agcattacgc gtccttgtaa
     6961 cgcgctacca accagattgc cgccttcacc gtggtctttt gcttctttgc ggttaaagca
     7021 gtacggcagg tccaggtcgt gatgctccgc cagtgccaca gcggttgtgg tggcaatcgg
     7081 gatccctttg taagcagggc caaacagcag atcgaactca atgccggaat ccaccaacgc
     7141 ttcagcgtaa aaacggccta acagtgccag atcgcgcccg gtattaaaca gcccggcgtt
     7201 gaagaaatag gggcttttgc gcccggattt cagcgtaaac tcgccaaact ttaacacctg
     7261 cttgctaagc gcaaattcaa taaactggcg ctgatatgat ttcatgcctt cgctcctcat
     7321 cttacttttc tacagacaaa aaaaaggcga ctcatcagtc gccttaaaaa tcagtttgcc
     7381 agcgccgcct tctgcgtcgc tacaatggat tcgattcccc ctcgggccag cgccaacaag
     7441 gtgagtagct cttcatgggt gaacggctcg ccttctgccg tcccctgcac ttcaatgatg
     7501 cgcccgtctt cggtcatcac tacgttcatg tcggtctctg cggctgagtc ttcaacgtat
     7561 tccagatcgc aaattgcttc accgtttaca ataccgacag aaactgcggc taccatccct
     7621 ttcatcgggt tggttttcag cttgccgttt tccaccagct tctgtagcgc atctgccagc
     7681 gccacgcagg cacccgtaat cgacgcggta cgagtgccac catcagcctg aagcacgtcg
     7741 cagtccagcg taatggtgaa ctcacccagc gctttcaaat ctactgccgc gcgaagagca
     7801 cgggcgatca gacgctggat ttccattgtg cgtccaccct gcttaccttt cgccgcttca
     7861 cgagcgttac gggtgtgggt agaacgtggc agcatgccgt actctgcggt gatccagccc
     7921 tggccctgac ctttcaggaa gcgcggcacg ccttcttcaa tagaggcggt acacaatact
     7981 ttggtatcgc caaattcgac cagcaccgag ccttctgcat gttttgtata gttacgagtc
     8041 agggtaacgg gacgcacctg attattgcta cggcctgctg gacgcatatt gaaatctccg
     8101 gcttgaaaca aatgtggctg cgcattatac ggacttccgg cggttattcc tatcctgaca
     8161 aggcatcgat ggctataatc cttccacctc tccttttata aacaggaacg tctatgatcc
     8221 gcagtatgac cgcctacgcc cggcgtgaaa tcaagggtga atgggggagc gcaacctggg
     8281 aaatgcgctc ggtaaaccag cgttatctgg aaacttactt tcgtctgccg gagcagttcc
     8341 gtagccttga acctgtcgtt cgcgagcgta ttcgttctcg cctgacgcgc ggtaaagtgg
     8401 aatgtaccct gcgctatgag ccagatgtta gcgcacaagg tgagctgatc ctcaatgaaa
     8461 aactggctaa acagctggta actgccgcga actgggtaaa aatgaagagt gacgaagggg
     8521 aaatcaaccc ggttgatatt ctacgctggc cgggcgtgat ggcagcccag gagcaggatc
     8581 tcgacgcgat tgccgctgaa attctcgcgg cgttggatgg tacgctggac gactttatcg
     8641 tcgcgcgcga aaccgaaggt caggcactga aagcattgat cgagcagcgt ctggaaggcg
     8701 tcaccgccga agtggtcaaa gtccgcgccc atatgccgga aattctgcaa tggcagcgtg
     8761 agcgtctggt cgcgaagctg gaagatgctc aggttcagtt ggaaaacaac cgtctggagc
     8821 aggagttggt tctgctggca caacgaattg acgttgccga agagctggac cgcctcgaag
     8881 cgcatgttaa agagacgtac aacattctga agaaaaaaga agcggttggt cgtcgtctgg
     8941 actttatgat gcaggagttc aaccgtgagt cgaacactct tgcgtcgaaa tctatcaatg
     9001 ccgaagtgac aaactccgcc atcgagctga aagtgttgat tgagcagatg cgcgagcaga
     9061 ttcagaatat tgagtaactc gtaacaccaa ttctgacttt tcagccttca caaatgccgc
     9121 ccactcaaac agagcggcat ttttcttccc cgcaacttca attctgtttt gcgtgcctgc
     9181 tccagatttt gcgatgtttt tttgcccagc acactgagaa cgtgagatac tcacaactgt
     9241 atataaatac agttacagat ttactttctt tgcaatcgat atcacatgga gtgggcaatg
     9301 aacgaacatc atcaaccttt tgaagagata aaactgatta atgcaaacgg agcagaacaa
     9361 tggtcagcaa gacaacttgg gaaactactg ggttattcag agtatcgtca ctttatacct
     9421 gtattaacgc gcgccaaaga ggcctgtgag aacagtggtc acacaattga tgaccatttc
     9481 gaggagatcc tcgatatggt caaaattggc tcaaatgcca aaagagcatt aaaagacatc
     9541 gtactctccc gctatgcctg ttacctggta gtacaaaacg gcgaccccgc gaaaccggtc
     9601 attgcggcag ggcagactta ttttactatc cagacccgac ggcaggagct tgctgacgat
     9661 gaagcattta agcaacttcg tgaagacgaa aagcgtctgt ttctgagaaa tgagttgaaa
     9721 gaacataaca aacaattggt tgaggcagca cagcaagctg gggtagcgac agctactgat
     9781 ttcgccatat ttcagaatca tggttaccag gggctgtatg gtggattaga tcagaaagcc
     9841 atccaccaac tgaaggggct gaaaaagagt cagaagatcc tggatcatat gggttcaaca
     9901 gaactggcgg ctaatctctt tcgagctacc caaacagaag aaaaactcaa gcgggatagc
     9961 gtgaattcaa aacagcaagc taataccaca cactttgacg tgggtagcaa ggtgaggcaa
    10021 accattcagg aacttggcgg aaccatacct gaggagttgc cgaccccgca ggtcagcatc
    10081 aagcagttgg aaaacagtgt aaaaattaca gagaagaaat agcagaaacg aaaaactctt
    10141 tttgttgatt gagacacccg atgcgtaagc caaggtccag gtgcagtgaa cacaatggct
    10201 aaacattgca cctttctttt cccccctcag ttttaaccta ttttttctta tgcattttct
    10261 cagacaagaa gtcagaagaa tgcatctacg ctacagaaaa tagcgatttc acataactaa
    10321 aagttatcaa tttcctctca ccttaaaaaa tatcaatcgt gacaatgcgc acaaatcgct
    10381 accctgccag acagattttt agggagagaa ccatgctgtt acacattttg tatttggttg
    10441 gcattactgc cgaagccatg acgggggcgc tggcggccgg gcgacggcgc atggatacat
    10501 ttggcgtaat tattattgca accgccaccg caattggcgg agggtcagtg cgcgatattc
    10561 tgctgggcca ctatccgctc ggctgggtca aacacccgga atatgtgatt atcgtcgcta
    10621 ccgccgcagt actcaccact atcgttgctc ctgtaatgcc ttacctgcgc aaagtgtttc
    10681 tggtgctcga tgcgctcgga ctggtggtct tttctatcat cggcgcacag gttgcactgg
    10741 atatggggca cggcccaatt attgccgttg tcgcggcggt aaccaccggt gtatttggcg
    10801 gcgttttgcg cgatatgttc tgtaaacgca ttccgctggt ctttcaaaaa gagttgtatg
    10861 ccggtgtctc atttgcctcc gccgtgctgt acatcgcgct gcaacactat gtttctaacc
    10921 atgatgtggt gattatctcc accctggtat tcggcttttt tgctcgttta ctggcgctac
    10981 gtcttaagct gggattaccg gtcttttact acagccacga aggtcactaa ggttcaaaac
    11041 ctgtgatctg ctgggcagcc agccaactgc ccagcttctt gatttgcgca ttttccttcc
    11101 attcaataac ctgtctggcg cgcccagatc cagtccccgg cagctgctgc cagaactgct
    11161 ccgtgctaaa taaaagttgc gaccaggacc gttcatcact ggcattaagc gccgcccggg
    11221 ttagcggtat tcccattgcc atcacccagc gagtaaaagg ctggttacga gccagattaa
    11281 actgatgcca taactgcgca cttttacttt tcgcgatccc cggcgtgttc tgtaattgct
    11341 ctggcgttaa taaaagccag gaaaagatat gctcaaagcg atgagtctga tgcagcgcgc
    11401 gccaaccggc ctcaccaatg ccatccagcc ccagaacctg ttttgacccc agccagacta
    11461 agcgtgaaat gaactgttcc tgacaaacat cagaagcaaa gtagcaggtc aacgggttaa
    11521 agcggttttc tggtggtgtc ggttttgtac gttctgcacc gcgccagacc acatcatcaa
    11581 tgcgaggaat accctgaccg gcaaggctga cgaggatctg atcaccaggc gcaatatccc
    11641 actcctgcca gcgcctgacg gaaccaatat tcacccgctg gactttttta tcatccagca
    11701 tgacaggtgc gagtgacgca accaccgata ttttaccgct cttacccacc gcaaactgaa
    11761 tcgccttcac ttcggcaacc tgagctacag gttgatattt ccaggccacc agccactccg
    11821 cctggcccgg tagccaatga cgggattcgg gttccttagc ccctcgcaca accacgccat
    11881 cggtgacgaa gggtaatttc gctttccacc actcattgcg tacgcgcgca acctcatcag
    11941 catttttaac cgcacgggta tacctctgcg ttagagtaaa acctgcggta gccagctcct
    12001 ttaaacgatc ggtcattaac tgcggtccat ccggccatgc ccagacaaaa acagccagtg
    12061 agtttagcgt gtcgctattc ccctggcgca tcatcaggcc agcaactttt gcgcgggcat
    12121 ttattccccc catttgttgt tggatatgcc cctcgcgctg gagaaatatt tccccctgaa
    12181 gcgtactgtt ggctaaaggc ccgctaacgg tttgcagcac agcggaaatt aagctaactt
    12241 tctgcgtcca gtcctcgcct ttcaggccat taccgcgact gattgctttg ttcagtttcc
    12301 cgtcccgata aaccagggtt accgcaacgc catcaacttt tggctgcacc caaagatcgc
    12361 tacgttctcg catccacaga cttaatgcgt ttttatccgc cattttgcgt acacccgtat
    12421 gggcaaccgg atgcataacc gcaccattta aaggcggcat catcacgtcg cggggctcgc
    12481 tcacaaagca gcgttgccac tgcgtaagac gggcgcttaa ctgatcgtaa acaccgtctt
    12541 ccacctcact ttttccttcc ttccagtagt catcgtccca ctgttttatt tgctgttgca
    12601 ggcgggaaat ttcttcctgt gctctggctg gcgaccaggc cggacagacc gcccacacag
    12661 atgattgcca gcacaagata cttattaata tcgccatcca tactttcatc ttcacctccg
    12721 ctgtagtcag gctggcagat atacaacgtg gtgaaggcaa agccgagtgg caaaaacgga
    12781 gtctgcgagg acgcttcctg agaatcgtct ttattgcagt aaatcacagg caaatgcgga
    12841 aacagctacg caaaacgcaa caactttgcg caaaaagtgt gagcaagggc tacgtcacat
    12901 ggccgcgccg tgtataataa gctcgtatgt aggctttatt tcgctaatca catacgaaag
    12961 atactcatgg ctcaaggcac gctttatatt gtttctgccc ccagtggcgc gggtaaatcc
    13021 agcctgattc aggctttatt aaaaacccaa ccgttgtatg acacccaggt ttctgtttca
    13081 cacaccacac gccaaccgcg tcctggtgaa gtccacggtg aacattattt ctttgttaat
    13141 catgatgaat ttaaagaaat gattagcaga gatgcgttcc tcgaacacgc agaagttttt
    13201 ggtaattact atggcacttc gcgtgaggcc attgagcaag tactggcgac cggtgtcgat
    13261 gtttttctcg atatcgactg gcagggcgcg cagcaaattc gccagaagat gccgcacgcg
    13321 cggagtatct ttattttacc gccgtccaaa attgaactgg accgccgtct acgcggtcgc
    13381 ggtcaggaca gcgaagaggt cattgcaaag cgtatggcgc aagctgttgc agaaatgagc
    13441 cattacgccg aatatgatta tctgattgtg aatgatgact tcgataccgc gttgaccgat
    13501 ttgaagacca ttattcgcgc cgaacgtctg cgcatgagcc gccaaaagca gcgtcatgac
    13561 gctttaatca gcaaattgtt ggcagactga acctgatttc agtatcatgc ccagtcattt
    13621 cttcacctgt ggagcttttt aagtatggca cgcgtaactg ttcaggacgc tgtagagaaa
    13681 attggtaacc gttttgacct ggtactggtc gccgcgcgtc gcgctcgtca gatgcaggta
    13741 ggcggaaagg atccgctggt accggaagaa aacgataaaa ccactgtaat cgcgctgcgc
    13801 gaaatcgaag aaggtctgat caacaaccag atcctcgacg ttcgcgaacg ccaggaacag
    13861 caagagctgg aagccgctga attacaagcc gttaccgcta ttgctgaagg tcgtcgttaa
    13921 tcacaaagcg ggtcgccctt gtatctgttt gaaagcctga atcaactgat tcaaacctac
    13981 ctgccggaag accaaatcaa gcgtctgcgg caggcgtatc tcgttgcacg tgatgctcac
    14041 gaggggcaaa cacgttcaag cggtgaaccc tatatcacgc acccggtagc ggttgcctgc
    14101 attctggccg agatgaaact cgactatgaa acgctgatgg cggcgctgct gcatgacgtg
    14161 attgaagata ctcccgccac ctaccaggat atggaacagc tttttggtaa aagcgtcgcc
    14221 gagctggtag agggggtgtc gaaacttgat aaactcaagt tccgcgataa gaaagaggcg
    14281 caggccgaaa actttcgcaa gatgattatg gcgatggtgc aggatatccg cgtcatcctc
    14341 atcaaacttg ccgaccgtac ccacaacatg cgcacgctgg gctcacttcg cccggacaaa
    14401 cgtcgccgca tcgcccgtga aactctcgaa atttacagcc cgctggcgca ccgtttaggt
    14461 atccaccaca ttaaaaccga actcgaagag ctgggttttg aggcgctgta tcccaaccgt
    14521 tatcgcgtaa tcaaagaagt ggtgaaagcc gcgcgcggca accgtaaaga gatgatccaa
    14581 aaaatcctct cggaaatcga agggcgtttg caggaagcgg gaattccctg ccgcgtcagt
    14641 ggtcgcgaga agcatcttta ttcgatttac tgcaaaatgg tgctcaaaga gcagcgtttt
    14701 cactcaatca tggacatcta cgctttccgc gtgatcgtca atgattccga cacctgttat
    14761 cgcgtgctcg ggcaaatgca tagcctgtac aagccgcgtc cgggtcgcgt gaaagactac
    14821 atcgccattc caaaagcgaa cggctatcag tctttgcaca cctcgatgat cggcccgcac
    14881 ggtgtgccgg ttgaggtcca gatccgtacc gaagatatgg atcagatggc ggagatgggt
    14941 gttgccgcgc actgggctta taaagagcac ggcgaaacca gcaccaccgc gcaaatccgc
    15001 gcccagcgct ggatgcaaag cctgctggag ctgcaacaga gcgccggtag ttcgtttgaa
    15061 tttatcgaga gcgttaaatc cgatctcttc ccggatgaga tttacgtttt cacaccggaa
    15121 gggcgaattg tcgagctgcc tgccggtgca acgcccgtcg acttcgctta tgcagtgcat
    15181 accgatatcg gtcatgcctg cgtgggcgca cgcgttgacc gccagcctta cccgctgtcg
    15241 cagccgctta ccagcggtca aaccgttgaa atcattaccg caccgggcgc tcgcccgaat
    15301 gccgcgtggc tgaactttgt cgttagctca aaagcgcgcg ccaaaattcg tcagttgctg
    15361 aaaaacctca agcgtgatga ttctgtaagc ctgggccgtc gtctgctcaa ccatgctttg
    15421 ggtggtagcc gtaagctgaa tgaaatcccg caggaaaata ttcagcgcga gctggatcgc
    15481 atgaagctgg caacgcttga cgatctgctg gcagaaatcg gacttggtaa cgcaatgagc
    15541 gtggtggtcg cgaaaaatct gcaacatggg gacgcctcca ttccaccggc aacccaaagc
    15601 cacggacatc tgcccattaa aggtgccgat ggcgtactga tcacctttgc gaaatgctgc
    15661 cgccctattc ctggcgaccc gattatcgcc cacgtcagcc ccggtaaagg tctggtgatc
    15721 caccatgaat cctgccgtaa tatccgtggc taccagaaag agccagagaa gtttatggct
    15781 gtggaatggg ataaagagac ggcgcaggag ttcatcaccg aaatcaaggt ggagatgttc
    15841 aatcatcagg gcgcgctggc aaacctgacg gcggcaatta acaccacgac ttcgaatatt
    15901 caaagtttga atacggaaga gaaagatggt cgcgtctaca gcgcctttat tcgtctgacc
    15961 gctcgtgacc gtgtgcatct ggcgaatatc atgcgcaaaa tccgcgtgat gccagacgtg
    16021 attaaagtca cccgaaaccg aaattaatgt tttatgaacc caacacgtta tgcacgcatc
    16081 tgcgaaatgc tcgccaggcg gcagcctgat ctgaccgtct gcatggagca ggtccacaaa
    16141 cctcataacg tttctgcgat tattcgtacc gcagatgccg ttggcgtaca tgaagttcac
    16201 gccgtctggc ctggtagccg catgcgcacc atggcttcgg cagcggcggg tagtaacagc
    16261 tgggtacagg tgaaaacaca ccgcaccatt ggcgatgccg tcgctcatct caaaggccag
    16321 ggcatgcaga ttctggcaac ccatctttct gataatgctg tcgatttccg cgaaattgat
    16381 tacactcgcc cgacctgcat tttgatggga caggagaaaa cgggcatcac gcaggaagca
    16441 ttggccctgg cggatcagga catcatcatt ccgatgatcg gcatggtgca gtcgctgaat
    16501 gtttccgttg cctcagccct cattctttac gaagcccagc gtcagcggca aaatgcaggc
    16561 atgtacctgc gtgaaaacag catgttgccg gaagcagagc aacaacgcct gttgtttgaa
    16621 ggcggctatc cggtgctggc gaaagtcgca aaacgcaaag gcctgcctta tccccacgtc
    16681 aatcagcaag gcgagatcga agctgatgcc gactggtggg ctactatgca ggctgcaggg
    16741 taagtgccat gaaaggtcgc ctgttagatg ctgtcccact cagttcccta acgggcgttg
    16801 gcgcagcact tagtaacaaa ctggcgaaaa tcaacctgca taccgtgcag gatctactct
    16861 tacaccttcc cctgcgctac gaagatcgca cccatctcta ccccatcgga gaactactgc
    16921 cgggcgttta tgccacggtg gaaggcgaag tgctgaactg caatatctcc ttcggcggtc
    16981 ggcggatgat gacctgccag atcagcgacg gttccggcat cctcaccatg cgctttttca
    17041 atttcagcgc ggcaatgaaa aatagcctgg cgacgggccg ccgtgtactg gcttatggcg
    17101 aagcaaagcg cggtaaatat ggtgcggaga tgatccaccc ggaataccgc gtgcagggcg
    17161 atctcagcac gccagaatta caggaaacgc tcacgccggt ttatccaaca acggaaggcg
    17221 taaagcaggc cacgctgcgt aaattaaccg accaggcgct ggatctgctc gacacctgcg
    17281 ccattgaaga actcctgccg ccggaactgt cacaaggaat gatgacgcta ccggaagcgt
    17341 tgcgcacttt gcaccgcccg ccaccgacgc tacagcttag cgatctggaa accgggcagc
    17401 atccggcgca acgtcgtctg attctggaag aactgctggc gcacaacctc agcatgttag
    17461 ccttacgtgc cggagcacag cgttttcatg cccagccgct gagcgccaat gacacgctga
    17521 aaaataaact cctcgccgcc ttaccgttca agccaacggg cgcacaggca cgcgtagtgg
    17581 cggagatcga gcatgatatg gcgctggatg tgccgatgat gcgcctggta cagggcgatg
    17641 taggttccgg taaaacgctg gtcgccgccc tcgccgcgct gcgtgcgatt gcccacggca
    17701 aacaggtggc actgatggca ccaaccgaat tactcgccga gcagcacgcc aataacttcc
    17761 gcaactggtt tgcaccgctc ggtatcgaag tgggctggct cgccggtaag cagaaaggca
    17821 aagcgcgact gtctcagcag gaagccatcg ccagcggtca ggtgcagatg attgtcggta
    17881 cacacgccat cttccaggaa caggtgcagt ttaacggcct ggcgctggtg attatcgacg
    17941 aacagcatcg ttttggcgtg catcagcgtc tggcattgtg ggagaaaggc cagcagcagg
    18001 gcttccatcc gcatcagttg atcatgaccg ccacgccgat cccccgcacg ctggcaatga
    18061 cggcgtatgc cgatctcgat acctcggtga tagatgagct gccgccaggc cgcacgccag
    18121 tgactacggt cgctattcct gatacccgcc gtaccgacat cattgaccgc gtgcgccacg
    18181 cctgcataac tgaaggtcgt caggcgtact gggtttgtac gttgattgaa gagtcagagt
    18241 tactggaagc gcaggcggcg gaagctacct gggaagagtt gaaactggcg ctaccagaat
    18301 tgaatgttgg cctggtacac gggcggatga aaccagccga gaaacaggcg gtgatggcgt
    18361 cgtttaaaca aggtgagcta catctgctgg ttgccactac cgttattgaa gtcggtgttg
    18421 atgtgcctaa cgccagcctg atgattatcg aaaaccctga gcgtctgggt ctggcgcagt
    18481 tacaccagct gcgcgggcgc gtaggtcgtg gcgcggtggc ttctcactgc gtgctgctct
    18541 acaaaacgcc gctttctaaa acggcgcaaa ttcgcctgca agtgctgcgc gacagtaacg
    18601 acggttttgt gattgcgcaa aaagatctgg agattcgcgg ccctggcgaa ttgttaggca
    18661 cgcgtcagac gggtaatgct gaatttaaag tggcggattt actgcgcgat caggcgatga
    18721 tcccggaagt tcagcgcctg gcgcgacata ttcacgaacg ttacccacaa caggcaaaag
    18781 ccttgataga acgctggatg ccggagacag aacgttactc gaatgcgtaa aaggcggcgg
    18841 tgagaagacc gccgcttcat cggttaaccg gcaaaaatcg gcaacattaa atacagctta
    18901 atcaccagcg cattgacgat atcgataaag aacgcaccga ccatcggcac caccaaaaac
    18961 gccatgtgcg acgggccaaa gcgttcagtg atcgcctgca tgttggcgat tgccgttggc
    19021 gttgcaccga ggccaaaacc acagtgaccc gcagccagca ctgccgcatc gtagtttttg
    19081 cccatcatgc gccaggtaac gaagatggca tacaacgcca tgaagatggt ctgtaccacc
    19141 agaatcgcca gcatcggcag cgccagcgaa gccagctccc acagtttcag ccccatcaac
    19201 gccatcgcca ggaacaacga caagcttacg ttacccagca cggataccgc acgctcaaag
    19261 acgcggtaaa agcccattat tgacagaccg ttgctcagaa tcacgccaac aaacagtaca
    19321 cagacgaagg tcggcagttc aaaagcagtg ccagccaaaa gttgcgcaac aattttcccc
    19381 accgtcaggc agatagcaat cagcgcgata gtttcaatca gcaccaacga ggtgatcatg
    19441 cgtcccacat ccggcttttc aaacgccgtc gggacttcct ggtcatccgg aataccgttc
    19501 ggcgtggtgg agtgtttcac cagatagcgc gccaccggac cgccaatcaa gccgcccagc
    19561 accagaccga acgttgcaca ggccatcgcc acttccgtcg cattggtgaa gccataacgt
    19621 tcaatgaaca atttactcca cgcagcgccc gtaccgtgac cgccggaaag cgtaatagaa
    19681 ccggccaaca gccccatcag cggatcaagc cctaacaggc tagccatacc aatgccaatg
    19741 gcattttgca tcaccaacag accaacaacc acaatcaaga agatgccaac cacacgccca
    19801 ccggcacgca aactggcaat gttggcgttc aggccaatgg tggcgaagaa agccagcatt
    19861 aacggatcgc gcagggacat atcaaagttg acttcccagc ccatgctttt tttcagtact
    19921 agtagcgcca gcgccaccaa caaaccaccc gcaacaggtt ccggtatggt gtatttcttc
    19981 aaaaaggaga cggaatggac caacttacgc ccgagcagca acgtcagcgt tgcggcaaca
    20041 agcgttgcta aagtatcgag atgaaacata gttactcctt tgtatccgca tcactctttc
    20101 atactcgtca tacttcatcg cgcagtggca acttcatgaa ctatgacggg tatcgagtta
    20161 tgctttcctg ggtcaaattc tcagctgtta atgaacaaat ggcatggatt ttaagcagaa
    20221 aaataccaaa agttatataa aaaagtgcat ttatctcatt taaatattta gatgaggatg
    20281 tggcaaacgt ttgcttttct ctttcggtca gctaaaatgc ccgctttgct accacgggat
    20341 tgttttcgat gtctgtttcc accctcgagt cagaaaatgc gcaaccggtt gctcagactc
    20401 aaaacagcga actgatttac cgtcttgaag atcgtccgcc gcttcctcaa accctgtttg
    20461 ccgcctgtca gcatctgctg gcgatgttcg ttgcggtgat cacgccagcg ctattaatct
    20521 gccaggcgct gggtttaccg gcacaagaca cgcaacacat tattagtatg tcgctgtttg
    20581 cctccggtgt ggcatcgatt attcaaatta aggcctgggg tccggttggc tccgggctgt
    20641 tgtctattca gggcaccagc ttcaactttg ttgccccgct gattatgggc ggtaccgcgc
    20701 tgaaaaccgg tggtgctgat gttcctacca tgatggcggc tttgttcggc acgttgatgc
    20761 tggcaagttg caccgagatg gtgatctccc gcgttctgca tctggcgcgc cgcattatta
    20821 cgccgctggt ttctggcgtt gtggtgatga ttatcggcct gtcgctaatt caggtggggc
    20881 tgacctccat tggcggcggt tacgcagcca tgagcgataa caccttcggc gcaccgaaaa
    20941 atctgctgct ggcaggcgtg gttttagcct taattatcct gcttaaccgt caacgtaacc
    21001 cttacttacg cgtggcctca ctggtaattg cgatggcggc cggatatgcg ctggcgtggt
    21061 ttatgggcat gttgccagaa agcaacgaac cgatgacgca agaactgatt atggtgccaa
    21121 cgccgctcta ttacggtctt ggcattgaat ggagtctgct gctgccgctg atgctggtct
    21181 ttatgatcac ttcgctggaa accattggcg atatcacggc gacctctgac gtttccgaac
    21241 agccagtgtc cggtccgctg tacatgaaac gcctgaaagg cggcgtgctg gcaaacggcc
    21301 tgaactcgtt tgtttcggcg gtgtttaaca ccttcccgaa ctcctgcttc gggcaaaaca
    21361 acggagtgat ccagttgact ggtgttgcca gccgctatgt cggttttgtc gtcgcgctga
    21421 tgttgatcgt gctgggtctg ttcccggcag tgagcggttt tgtacaacac attccagaac
    21481 cggttctggg cggcgcaacg cttgtaatgt ttggcaccat cgccgcctcc ggtgtgcgta
    21541 tcgtttctcg tgagccgctg aaccgtcggg cgattctgat tatcgcgctg tcgctggcgg
    21601 ttggtctggg cgtgtctcag cagccgctga ttttgcagtt tgcccctgaa tggctgaaaa
    21661 acctgctctc ctccgggatc gccgcgggcg gtattactgc catcgtgctg aatctgattt
    21721 tcccaccaga aaaacagtaa ttcactggcg gcggtaagat aattaccgcc gcagtctcac
    21781 ctctttcacc attcccgcct tgaggattgc gcgtaaatcg tgcataactc ccttatgtgc
    21841 atttcacggg atggaagacc atgaaattta ttgggaagct gcttctctac attctcatcg
    21901 ctctgttagt ggtgatcgct ggcctctatt ttcttctgca aacccgctgg ggagcagaac
    21961 atatcagcgc atgggtttcc gagaatagcg actatcatct ggccttcggg gcgatggatc
    22021 accgtttttc cgcgccatct catatcgtgc tggagaatgt cacgtttggt cgtgatggtc
    22081 agcccgcgcc cctggtggca aaaagtgtcg acattgcgct aagcagtcgc caactgaccg
    22141 aaccacgcca tgtcgatacc atcctgctgg aaaacgggac gctgaatctc accgaccaga
    22201 ccgcgccgct accgttcaaa gccgatcgtc tgcaactgcg tgatatggcg tttaatagcc
    22261 cgaatagcga atggaaactg agcgcgcagc gggtaaatgg cggcgtggtt ccgtggtcac
    22321 cagaagccgg taaagtgctg ggtacgaagg cgcagattca gtttagtgcc ggatcgcttt
    22381 cgctcaatga tgttcctgcc accaatgtac tgattgaagg cagtattgat aacgatcgcg
    22441 ttacgctgac taacctgggt gccgacatcg cccgcgggac attaaccgga aacgcgcagc
    22501 gtaacgccga cggcagctgg caagtggaaa atctgcgcat ggcggatatc cgtctacaaa
    22561 gcgaaaaatc gctaaccgac ttctttgcgc cattacgctc tgtcccgtcg ttgcagattg
    22621 gtcgcttgga agtcatcgat gctcgtctgc aaggtccgga ctgggcggtg accgacctcg
    22681 atctcagctt gcgcaacatg accttcagta aagatgactg gcagacacag gaaggcaaac
    22741 tgtcgatgaa cgccagcgag ttcatttatg gttcgctgca tttatttgac ccgattataa
    22801 acgcggaatt ttccccgcag ggcgtagcgc tgcgccagtt caccagccgc tgggaagggg
    22861 gtatggtcag aacgtcaggg aactggctgc gtgacggtaa aacgttgatc cttgatgatg
    22921 cggcaattgc cgggctggaa tataccttgc cgaaaaactg gcaacagttg tggatggaaa
    22981 cgacacccgg ttggttaaac agcctgcaac tgaagagatt tagcgccagc cgcaatctga
    23041 tcattgatat cgaccctgac ttcccgtggc agctcaccgc gctcgatggt tacggtgcca
    23101 acctgacgct ggttaccgat cataaatggg gcgtctggag tggctcggcg aatctgaatg
    23161 ccgccgccgc gacattcaat cgtgttgatg ttcgtcgccc gtcgctggcg ctgaccgcca
    23221 acagcagcac ggtgaatatc agcgaactga gtgcatttac tgaaaaaggc attctggaag
    23281 ccaccgcaag tgtttcacaa acgccacaac gtcagaccca tatcagcctg aatggacgcg
    23341 gtgtgccggt gaatattttg caacaatggg gatggcctga attaccgttg actggcgacg
    23401 gcaatattca gcttaccgcc agtggagata ttcaggccaa tgccccgctg aaacctacgg
    23461 ttagcgggca attgcatgcc gtgaacgccg caaagcagca agtgactcaa accatgaatg
    23521 cgggcgtcgt ttccagcggt gaagttacat cgacggagcc ggtgcggtaa acatcaatct
    23581 cgccgggtac gactcagacg tacccggcat tccgtcaata gattacaacg taattgtcag
    23641 cgcattccct tgaggcgtca ccaccagccc ctgctcactt tcagcctgcg aaccgccttg
    23701 cagaccattt actttcacaa cattgcgcaa gcacagcgtc cagttcttcg cctcgcccgc
    23761 accagtcaca gtaatcgtgt tgccagtacg tgctgctttt aaagtaaaga tcaccgatcc
    23821 gtcagcagca ggcacttcac agacggcttc atgcccgtct tgcagattaa agaggtggaa
    23881 tgccgtgcct tcgtgccaca cgtaatcggg acgttgatcg ttgttgccca gcgccagtag
    23941 agtgttatca cgcacataaa cgggcagact caggaagccg tgctgctgtt tatgccagcg
    24001 actaccgtcg agttcatcgt tgtgccacag gtgtgtccag cgaccttccg gcaggtagaa
    24061 ctgcacatcg cccgcttcag tgaacaccgg cgcaaccatc acgttgtcgc ctaacatgta
    24121 ttgacggtca aggtaatcac aagccggatc gtccgggaac tccatcatca tggcccgcat
    24181 catcggcgta ccccgcgcgt tcgcacgcgc agcttcacga tacagatacg gcatcatgcg
    24241 gcatttcagt tgcgtgaaga agcgcaccac atcacaggac tcatcatcgt aggcccacgg
    24301 cacacgataa gatttgctac cgtgtaaacg gctatggctg gagagcaaac caaacgcgca
    24361 ccagcgtttg taaacgtgcg ccggagcggt attttcaaag ccgccgatat cgtggctcca
    24421 gaagccaaaa cctgaaaggc caatagacaa accaccgcgc aggctttccg ccattgattc
    24481 gtagttagcg taacaatcgc caccccagtg taccgggaat ttctgcgcac cgacggaggc
    24541 cgagcgggca aacaagacag cttcttcctc accaacggtg tccttgagca cgttccacac
    24601 cagttcgttg tagatgtacg cataatggtt atgcattttc tgcgaatcgg aaccgtcaaa
    24661 ccactgaaca tcaattggga tacgttcgcc aaagtcggtc ttaaagcaat caacgcccat
    24721 cgcgaccaga cctttcagtt tgtcggcgta ccatttgcag gcatccggat tggtaaagtc
    24781 ataaatcgcc agacctggct gccatttatc ccactgccat agcgaaccgt ccgggcgttt
    24841 gagtaaatag cctttctctt gtaactcttt aaagacgggg gatttttgac cgatataggg
    24901 gttaatccag acgcagattt tcagcccttt cgctttcagg cggcggatca tcccttccgg
    24961 gtcagggaaa gtcagcgggt cccactcaaa atcgcaccac tggaaggctt tcatccagaa
    25021 acagtcaaag tggaaaacat gcagcggcag attgcgttcc gccataccat cgataaagct
    25081 gtttaccgtc gcttcgtcgt agttggtggt aaatgaagtg gttagccaca ggccgaagga
    25141 ccacgggggc ggcagcgccg gacgaccagt aaagcgggta taacgatcaa gtaccgcttt
    25201 cggcgtcggg ccgtcgataa caaagtattc gagatattca ctctcaacgc tgaactgcac
    25261 tttggagact ttctccgatc ccacttcaaa agagacacac tggggatgat tgaccagtac
    25321 cccataacca cggttagtca tgtagaacgg gatattttta tacgcctgtt cagtacttgt
    25381 gccgccgtcc cggttccagg tctctaccgt ctggccattg cgcaccaggg cagtaaagcg
    25441 ctctcccaga ccgtaaactg tttcgccaac gccaagatca agccgctcaa acatataatt
    25501 gcgttgatta ttcgtgtcct gcacgtagcc attatttttc acctgactac cggtaatacg
    25561 ttcgccgttg cgcagaaaat ccagtgacca gaactcacct ttgctgacac gcgcgcttaa
    25621 gttgccactt ttaaactcag cataacgttc tgtgttttcg attgtgacct tcacgtcctg
    25681 caaaatattg agcggataat gaggaccgtt attcagcgcc ccctgaaaat gctcaatccg
    25741 cacaccgaca ataccttcct gtggggagaa aaagcgcagc gtaaataaag gcgtatcaag
    25801 ctgccaggta cgttcacgca catcacgggg ggcagcatag accaccattt cattatcctg
    25861 ctgttcaacc tcgaatacct gaagcgggtg aatcaaattg aggccaggtt gaatcaacca
    25921 gtttccatcg ctaattttca ttgccgttcc ttagttctgc acttcttgag aggtgaattg
    25981 ttgctggcaa cggcgtttac cctgagccag ctgttccata acggttttca gattgtgcgt
    26041 cgtgagtgag tagtagcgtt tagcgataat cgcgctcagc aaataacaga tcgccggaac
    26101 aatcgtgaat agcgcaataa tgatgctaat cgtggcgctg ttctgcgctt tttctgccgc
    26161 atcatatccg ccataagcca gcatccagcc gataagagcg ccgccgaagg ccaaccccag
    26221 tttgagcaca aacagcgtgc cagcaaaact gatcccggtc aggcgtttac cattgcacca
    26281 ctcgccgtag tcgacggtat cgaacatcat tacccactgg ataggtgtca ccagttgatg
    26341 caacacacca atcacgaaga tgaagacaaa catagtgatg ctggcctgca tgggaacaaa
    26401 gaacatcgcg aggctaatca ctgccagcag ggcgttcgtc caccagaaga tagtgacttt
    26461 acatttccag tcggtcagag gttttgccag tgcggaacca atcaggttac ccacgcaata
    26521 agtggtgaga aaagcgacaa acacttccgg cgtgcccaaa atccatgtga cgtaatacat
    26581 catcgcccca ccgcgtacgc acaccgccag gatattgaaa atggttagta aaccgacaat
    26641 ccgccactgg tcgttttgcc agatatcacg taaatcttcc cgcatagacg ttgttgtagg
    26701 tggtgcttca acgcgttctt tagtggtgaa gaaacaaaat gccagcatca tgaatgccac
    26761 cacggaaagg accacgatac cgccctggaa accgagtggt ttattatcac cgccaattaa
    26821 attaaccagt ggcatcatca gaacagtaga aagcatgcct cccgcggtcg ccagcacaaa
    26881 acgccaggat tgcagcgaga tacgctgagt cgggtcattg gtgattacac cacccaatgc
    26941 gcagtaaggg atattgacga cggtataaag taaggtaagt tagggtgtaa gtaattgctg
    27001 catagatcat tttgccgttc atactgagat ctggcgtgct ataggccagt acacagacga
    27061 tcccgaatgg cagtgcgcca aacagtaccc acggacgaaa tttaccccag cgagagcgcg
    27121 ttcgatcggc caacaacccc atgcaaggat cggaaatcgc atccagtgca cgagcgacca
    27181 aaaacatggt tccgacaaat ccggcaggaa tgccaaaaat atcggtataa aagaacatca
    27241 tatataacat tacgttatcg aaaataatgt ggctggcggc gtctcccatg ccataaccaa
    27301 ttttctcttt aacggacaac acttcactcc tcatctgctt ttccttgatg ctataggggt
    27361 gctgagaccg ataacgcatt ttgtaaacca tccctgtgag agcgaatatt ccgatatctg
    27421 gttattaatt tatgattctt gttttatgtg atcgtggtag cgttaattcc gctcatatat
    27481 cattgtaaaa tatgggtttt atataaatta taatgctttc gtgataatac gctgcgttta
    27541 ttaggcggaa aaaactgatc tgggggatgt agaaactcaa ggaagtagct ataatgcgcc
    27601 ccgcctccat gtagcaatcg aggcgcggaa gatcgtcgtc tccggtgagg cggctggact
    27661 tcaaatccag ttggggccgc cagcggtccc gggcaggttc gactcctgtg atcttccgcc
    27721 agtttacctc ttctgaactc tcccaatgtc aataaatctc ttctaatccc ttgtatatca
    27781 gtatttattt gtatcctgag gtcaacctac ttcaccccgt atcaagtttc ttttgggggt
    27841 actttagggg gtgttatttc ctattgaaat acaagcaccc ccataatagg aaaaatgtga
    27901 tacataacaa cggcttttaa caatatcttt gttatacaag gggtgtattg ggggtatcag
    27961 gttagttact ggcagaaatc cccctcagga acatgaattc atcaaaagcc ggaggatgtc
    28021 atggcattaa cgatatcaaa gtgaaaaccg caaaacccaa agacaaaccc tataagcttg
    28081 ctgatggcgg cggtatgtat ctactgatta acactaatgg ttctaaatac tggcgcatga
    28141 agtaccgctt tgctggtaaa gaaaagatgc tttctattgg cgtatacccc gatgtaacgc
    28201 tagccgatgc tcgcgagaaa cgcagtgaag ccaggaaact cctcgctgct ggaggcgatc
    28261 caggggaggc aaaaaaggaa gaaaaaatcg cgcagcaaat gagtctgaaa aatacgttcg
    28321 aggctattgc tcgcgaatgg caccagttaa aagctgatcg gtggtcatta cgctatcgtg
    28381 atgaaatcat tgataccttt gaaaaagaca tttttcccta cattggcaag cgccctattg
    28441 ccgaaatcaa accaatgaag ttactggaag cactgcgtaa aatggaaaaa cgtggagctc
    28501 tggagaaaat gcgcaaggtc cgccagcgct gtggtgaggt gttccgctat gcaattgtta
    28561 ctggccgggc agactataac ccagcccccg atcttgccag tgctttagct acaccgaaaa
    28621 aagtacattt tccctttctt actgctaatg aacttcccca cttcctcaac gatctggcgg
    28681 gttataccgg aagtataatc actaagacag ctactcagat cattatgtta accggcgtac
    28741 gaacacaaga actgcgtttt gcacgctggg aagatatcga ttttgagaca aaattgtggg
    28801 agatcccggc agaagtaatg aagatgaagc gacctcatat tgtgccgctc tctgagcagg
    28861 tcattatgct atttaagcag cttgaaccaa tctcaaaaca tcatccccta gtctttatcg
    28921 gtagaaatga tccccgcaaa ccaattagta aggaaagcat taaccaagta attgaattac
    28981 ttggatataa gggaagactc acagggcacg gtttcagaca caccatgagt acaattctgc
    29041 atgaacaagg ctttaattcc gcctggattg aaatgcagtt agctcatgtg gataaaaatt
    29101 ccatcagggg tacctataat catgccctgt atctcgatgg ccgccgtgaa atgatgcaat
    29161 ggtacgctga ttacattgat tcactctcca gccgggaaag ttaagtcaga aatacttttc
    29221 cctgatgttt tcctcaatta agtattttat cctctggcct acaaggctgg aggatttttg
    29281 ttattatatt atttttttgc gtcttcaaac ttattacaaa aacataatta aaaacaaatt
    29341 gacattttta aattggcaat tacaaaatac agccttgcag ttatttcgat acctttagca
    29401 aaggaatagt tgattcagat ataatttagc atttcaatca tctgacatca ttataaaaaa
    29461 tgagattcac ataaagataa aattattgtt ctcaaggaaa gcatgaaggg gcttcgtaag
    29521 caatattcat tcggtgccaa gacaggactt gatacacaga atctcaactt ttatgctctc
    29581 agactatatc accacatgtt ttacaggctc agaaaatgaa cgatagatta tgctttgaag
    29641 tgcacgataa caagggttat ttcgtatttc cggatacctg gtttggccca ttgctggggg
    29701 agtttgaaga agtgcttgat gcatacgacg ctgatgaaat cagcgagacg agttacatca
    29761 acaaactccg acgccttgct caacgggagc cggactttat tgatatacac gctcatctgg
    29821 catatgcatt tctggaacag aatgccccgc gtaaggcact gaacgccgct cttaaaggac
    29881 tggcggcagg taatcgtatc atcccggaaa gtttctgtgg tgaaataatc tggatgcatc
    29941 ccgaaaaccg gccttatctg cgcgcgcttt atgcagcaat tctcgcaaat gtgcacttac
    30001 agcgccatca ggatgcggtt atgctcactg ataaaatact ggcatacaat cctgaagata
    30061 atcagggggc tcgctggttg ttgggttcag agctgttacg caccggcgat catgagcgag
    30121 ccttcagtgt actgaaagag catgctgatg aattttcacc ttactggtat gaactcggtc
    30181 tcctgcattt tctgaacggg gaacatgtta aggccgccac agcttttcgc catggctttg
    30241 ccacaaacac ttacatcgca gaaatgctgt gcggaaatct gcatccattt ccacttgctg
    30301 tgtggcatga cttctccggc tctctggata cggcagaaga ttactatgca acatactcac
    30361 cattatgggg acaatatccg gaagcattgc tgtttgtaaa ctggctctat aaccattcct
    30421 ccgtgctgca tgaacgatca gaaatcatta aatgcgccga aatgctgatt caggaagatg
    30481 atttcgaaat atgcgaaagt attttaagac agcaggagca cctgtggaaa agaattgaca
    30541 agacactttc tgaggaaatt gtacagaaat gcagaaatat gaacggtgaa tatatctggc
    30601 cctggatatt gcccttttcc gcagcaggga tgaaacattc aagcatccag tatcagtaga
    30661 tattgcatta gtgtaacttt aaaacaagta ataatttatt atccaataat aacactgtgt
    30721 tccttcccca ggtgattacc ctggggttat tttctgaaag tttcctttgc aaaaggatag
    30781 cgttgtaaat tactttaacg aattactcca tgaactcatt aaagttcttt cgtaatactt
    30841 tatcgtcaaa cacgttatag ctggcatcaa aaaccacaga tttaccctgc cagcgggtaa
    30901 tcccctcctc tggcgttttc ggttgaactg gtatccccgt ccaacacagg cagccattat
    30961 aaacagtaaa aaattgtatt cgctgttatg aaatcaatag tcctgacgta ctgttgaatg
    31021 tgcttgtgtt ctgaacctcg tacaaccagt acatttgata tcgaattata aacaaaacat
    31081 tgcatccaac ttttttaata tggacaatag ttgtccatac ggtggtttta tattcgataa
    31141 atcaatggat ttcattgagt cacaggactg gtaatggatg aaaatgcttt aaggtttgcc
    31201 tcatactggc gcaactcgct tgcagatgct gagtcaggaa agggctgttt tgaacggaaa
    31261 gacgcccaaa atttcactca ctggcatggg atagcggcgg gacgtcttga cgaagcgatt
    31321 gtcaataaat tttttaaggg agaaaaagac gatgtcgaaa ctgtcaatgt catcttacgc
    31381 ccaaaagttt atttccggtt actgcagcat ggtaaggacc gttccgcagg cacgcctg
//
LOCUS       DAJTQQ010000039        31169 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000039 DAJTQQ010000000
VERSION     DAJTQQ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31169
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.039"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            383..2041
                     /locus_tag="OO309_002008"
     CDS             383..2041
                     /locus_tag="OO309_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR5847159.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2038..2994)
                     /locus_tag="OO309_002009"
     CDS             complement(2038..2994)
                     /locus_tag="OO309_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR5847160.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3145..4260
                     /gene="macA"
                     /locus_tag="OO309_002010"
     CDS             3145..4260
                     /gene="macA"
                     /locus_tag="OO309_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR5847161.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4257..6203
                     /gene="macB"
                     /locus_tag="OO309_002011"
     CDS             4257..6203
                     /gene="macB"
                     /locus_tag="OO309_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR5847162.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6276..6500)
                     /gene="cspD"
                     /locus_tag="OO309_002012"
     CDS             complement(6276..6500)
                     /gene="cspD"
                     /locus_tag="OO309_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR5847163.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6823..7143
                     /gene="clpS"
                     /locus_tag="OO309_002013"
     CDS             6823..7143
                     /gene="clpS"
                     /locus_tag="OO309_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR5847164.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7174..9450
                     /gene="clpA"
                     /locus_tag="OO309_002014"
     CDS             7174..9450
                     /gene="clpA"
                     /locus_tag="OO309_002014"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR5847165.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9853..9940)
                     /locus_tag="OO309_002015"
     tRNA            complement(9853..9940)
                     /locus_tag="OO309_002015"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9904..9906),aa:Ser,seq:gga)
     gene            complement(10194..10412)
                     /gene="infA"
                     /locus_tag="OO309_002016"
     CDS             complement(10194..10412)
                     /gene="infA"
                     /locus_tag="OO309_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR5847166.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10697..11401)
                     /gene="aat"
                     /locus_tag="OO309_002017"
     CDS             complement(10697..11401)
                     /gene="aat"
                     /locus_tag="OO309_002017"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR5847167.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11443..13164)
                     /gene="cydC"
                     /locus_tag="OO309_002018"
     CDS             complement(11443..13164)
                     /gene="cydC"
                     /locus_tag="OO309_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR5847168.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13165..14931)
                     /gene="cydD"
                     /locus_tag="OO309_002019"
     CDS             complement(13165..14931)
                     /gene="cydD"
                     /locus_tag="OO309_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR5847169.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15054..16019)
                     /gene="trxB"
                     /locus_tag="OO309_002020"
     CDS             complement(15054..16019)
                     /gene="trxB"
                     /locus_tag="OO309_002020"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR5847170.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16563..17057
                     /gene="lrp"
                     /locus_tag="OO309_002021"
     CDS             16563..17057
                     /gene="lrp"
                     /locus_tag="OO309_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR5847171.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17192..21220
                     /gene="ftsK"
                     /locus_tag="OO309_002022"
     CDS             17192..21220
                     /gene="ftsK"
                     /locus_tag="OO309_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR5847172.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21375..21986
                     /gene="lolA"
                     /locus_tag="OO309_002023"
     CDS             21375..21986
                     /gene="lolA"
                     /locus_tag="OO309_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR5847173.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21997..23340
                     /gene="rarA"
                     /locus_tag="OO309_002024"
                     /gene_synonym="mgsA"
     CDS             21997..23340
                     /gene="rarA"
                     /locus_tag="OO309_002024"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR5847174.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23431..24723
                     /gene="serS"
                     /locus_tag="OO309_002025"
     CDS             23431..24723
                     /gene="serS"
                     /locus_tag="OO309_002025"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR5847175.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24963..27406
                     /gene="dmsA"
                     /locus_tag="OO309_002026"
                     /pseudo
     CDS             24963..27406
                     /gene="dmsA"
                     /locus_tag="OO309_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27417..28034
                     /gene="dmsB"
                     /locus_tag="OO309_002027"
     CDS             27417..28034
                     /gene="dmsB"
                     /locus_tag="OO309_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5847176.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28036..28827
                     /locus_tag="OO309_002028"
     CDS             28036..28827
                     /locus_tag="OO309_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5847177.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28863..29489)
                     /locus_tag="OO309_002029"
     CDS             complement(28863..29489)
                     /locus_tag="OO309_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5847178.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29804..30952
                     /locus_tag="OO309_002030"
     CDS             29804..30952
                     /locus_tag="OO309_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5847179.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7686 a         7643 c         8367 g         7473 t
ORIGIN      
        1 ttttataggg tcgtcgtaaa aaatgacgac ggaaataatg cgcggctatt ttaaaaacga
       61 aggcgagtca ttcaccagat aaataaatcc agtaaatttg atttagggca acagcgggtt
      121 gccccatata gtcatttgtc tgattgacag tgtagtgcaa gcaaaagatt taatccttta
      181 ggcgtaataa aaaataattt atcatgctaa ttatttgatt ttgttgtttt tgcgggctta
      241 tcagcaagag ggagtataac gcgattattc gctcattttt cagacaattg ccatgcttaa
      301 atgtgatgtc atcacgtatt agcaaggcct ttcccgttat actgcgagcg taaaggataa
      361 gtcacatatt tctggagggg atatgattct tgagcgcgtt gaaattgtgg gttttcgcgg
      421 tatcaaccgt ttgtcgttga tgctcgaaca aaacaacgtc ctgattgggg agaacgcgtg
      481 gggtaaatcc agcttgctgg acgccttaac cctgctgcta tcgccagaat cagatctcta
      541 ccattttgag cgcgacgatt tctggttccc gccgggagat atcaacgggc gagaacatca
      601 tctgcatatt attttgacct tccgcgaatc gctgccaggc cgacatcggg ttcgccgtta
      661 tcggccgctg gaagcgtgct ggacgccatg caccgatggc tatcaccgta ttttttatcg
      721 tctggaaggg gagagtgcgg aagacggcag cgtgatgaca ctgcgcagtt ttctcgataa
      781 agacggacat ccgattgatg ttgaggatat taacgatcag gcacgccatc tggtgcgttt
      841 aatgccggtg ctgcgcttgc gtgatgcccg ttttatgcgc cgtattcgta acggcacggt
      901 gccaaatgtc cccaatgtgg aagtcaccgc gcgccagctc gatttcctcg cccgtgagtt
      961 gtcctcacat ccgcaaaatc tctctgatgg acagattcgt cagggacttt ccgcaatggt
     1021 gcagttgctt gagcattatt tctctgagca gggggctgga caggcgcgat atcgtttaat
     1081 gcggcggcgg gccagcaatg agcaacgaag ctggcgctat ctggacatca tcaaccggat
     1141 gattgaccga cctggagggc gctcgtatcg ggttattttg ctcggcctgt ttgctacttt
     1201 attgcaggca aaaggcacat tgcgactgga taaagacgcg cgtccgttat tgctaattga
     1261 agatccagaa acccgcctgc atccgattat gctttccgtt gcctggcatc tgttgaatct
     1321 tttgccttta cagcgtattg ctaccaccaa ctctggcgag ctgctttcgt taactccagt
     1381 ggaacatgtt tgccgcctgg tgcgtgaatc ttctcgtgtt gccgcctggc gtctggggcc
     1441 aagtggcttg agcaccgaag acagccgccg catctctttt cacattcgtt ttaatcgtcc
     1501 gtcatcgctg tttgctcgct gctggttgct ggtggaaggg gaaacggaaa cctgggttat
     1561 caatgaactg gcgcgtcagt gcggacatca ttttgatgcc gaagggatca aggtcattga
     1621 gtttgcccag tccgggctaa agccactggt taaatttgcc cgccgaatgg ggattgaatg
     1681 gcatgtactg gtcgatggcg atgaagcagg gaagaaatat gccgctacgg tacgcagcct
     1741 gttgaataac gatcgggaag ccgaacgaga acatttaacg gcgttaccgg cgctggatat
     1801 ggaacatttt atgtatcgcc agggattttc cgatgtgttc caccgcgtgg cgcaaatccc
     1861 ggaaaatgta ccgatgaatc tacgcaaaat tatctcgaaa gcgatccatc gctcttccaa
     1921 acccgatctt gccattgaag tggcaatgga ggcaggacgt cgtggtgtgg actccgtacc
     1981 aacgctgctg aaaaaaatgt tctcacgcgt gctgtggctg gcgcgcggtc gcgcggatta
     2041 accgcgaaac atcgtggcca tttgtggctg aatagcgtcg agcatctcat agcgccgacg
     2101 gtattcagcc cgttttttac tggcgatttc ggcaatctct tttcgtgcta tctgtgctgg
     2161 aaggcggtaa tggcgttcag catcacatac gccgccaacc gactcccaga acgcgttgta
     2221 atcagcatgg atcttgcctt ctttatcgcg ataacgcagg ctgcggtaaa tatgcgtttc
     2281 attgctgacg gcaataatct gctctacctg caaacgttgg gcaaacagac aggccgcttc
     2341 catcacgagg cgtttgggaa atagcccgtg gcaggctttc gtcgcattct ggatttcctg
     2401 atgtggaatt tcccattttg cgccttgcag tccgccaata aacatcgttc ttttcccctg
     2461 atattcacac agggtaaacg tgatctctgc cagaggaata ccttcgctgt tgcggaacag
     2521 gattgtgctg tcaccttctt tatccattga gatcatcatg gtcagctcaa gcgtgaactg
     2581 ctcaccgttt ttgccttcca gtttagccag ttgcagcccg ggggtattca aatataagct
     2641 gaattcttcc gccgacatac acccgcggag taacgcataa tggtaacgta acgcctccag
     2701 caattgctta cggctaagat tcgcggcaag gtaagggcga tgcagacgca caggcagtcg
     2761 cggctggcgc gttaacaaca cattgagatt aggccagtgg gaaagttcgt tcatccactc
     2821 aacgcttaaa cgcggcataa tcaacgagcg cagcaaaaat ttctggcgaa aactacagcg
     2881 atgccagaat ttacccggcc gacactgtcc acgtgccaga ctaagaaaaa gtgacaggct
     2941 gctgagagat tcagatggcg taaaggtccg ttcagttagc tgcgacatat tcatgaaatc
     3001 aatggttata catgacgtcg atttcaccat tgcgtatctt aaccaaacat caatagtgtg
     3061 attactaacg taaattttag ggttttgttg atatttcgtt gaagttaatg acccggattg
     3121 gcatatggag tattcagaat atttatgaaa aagcggaaaa ccgtgaagaa gcgttacgtt
     3181 attgcgctgg tgatagtcat cgccggactg attacgttat ggagaattct taacgcaccc
     3241 gtgccgactt atcagacact gattgtgcgc cccggtgatt tacagcaaag cgtgctggcg
     3301 accggaaagc tggacgcgct gcgtaaggtt gacgtgggcg cgcaggtcag cggtcagttg
     3361 aaaacgctgt cggtggcgat tggcgataaa gtaaaaaaag accagctttt aggggtcatt
     3421 gatcctgaac aggctgaaaa ccagatcaag gaggtcgaag caacgctgat ggagctacgc
     3481 gcgcagcggc agcaggcgga agcggagctg aaactggcgc gggtgacgta ttcccgtcag
     3541 caacgtctgg cacaaacgca agcggtttca ctgcaggatc tcgataccgc cgcgacggag
     3601 atggctgtga aacaggcgca aattggcacc attgacgcgc aaatcaagcg caatcaggcc
     3661 tctctcgata cggctaaaac caatctcgat tacacccgta tcgttgcccc gatggctggg
     3721 gaagtgacgc aaatcaccac tctgcaaggc cagacggtga ttgccgcaca gcaagcaccg
     3781 aacattctga cgctggcaga tatgagcact atgctggtaa aagcgcaggt ttctgaagcg
     3841 gatgtaatcc acctgaagcc ggggcaaaaa gcctggttta cggtgcttgg cgatccactg
     3901 acgcgctacg aggggcaaat caaggatgtg ctaccgacgc cggaaaaggt aaacgacgct
     3961 attttctatt acgcccgttt tgaagtaccc aaccccaatg gtttgctgcg gctggatatg
     4021 accgcgcaag tgcatattca gctcaccgat gtgaaaaatg tgttgacgat ccctctgtcg
     4081 gcgttaggcg atccggttgg cgataatcgt tataaagtca aattgttgcg taatggtgaa
     4141 acacgcgagc gtgaagtgac gattggcgca cgtaacgata ccgatgttga gattgtcaaa
     4201 gggctggaag cgggcgatga agtggtgatt ggtgaggcca aaccaggagc tgcacaatga
     4261 cgcctttgct cgaattaaag gatattcgtc gcagctatcc tgccggtgat gagcaggttg
     4321 aggtgctgaa gggcatcagc ctcaatattt atgcgggtga gatggtcgcg attgttggcg
     4381 cttcgggttc cggtaaatcg accctgatga atattctcgg ctgcctggat aaggccacca
     4441 gcggcaccta tcgcgtagcc ggtcaggata ttgccacgct ggacgccgat gcgctggcgc
     4501 aactgcgccg cgagcatttc ggctttattt tccagcgtta ccatttgctt ccgcatttaa
     4561 ccgtcgagca gaacgttgaa gtacccgccg tctatgccgg tcttgagcgg aaacagcgac
     4621 tgcttcgtgc ccaggagttg ctgcaacggc tggggctgga agaccgtaca gagtattatc
     4681 cggcacagct ttcgggtggt cagcaacagc gcgtcagcat cgcgcgggca ttgatgaacg
     4741 gcggtcaggt aattcttgcc gatgaaccga ccggcgcact ggacagccat tctggcgaag
     4801 aggtgatggc gatcctgcat cagctgcgcg atcgcgggca tacggtgatt atcgtcaccc
     4861 acgatccgca ggtcgctgct caggccgagc gggtgatcga aattcgcgac ggcgaaattg
     4921 tgcgcaatcc tcccgccatt gaaaaagtga atgttgctgg cgggacggag cctgttgtca
     4981 atacagtgtc tggctggcgg cagtttgtca gcggttttaa cgaggcgctg acgatggcat
     5041 ggcgggcgct ggcagcgaat aaaatgcgta ctttactgac catgctgggg attattatcg
     5101 gtattgcgtc ggtggtttcc attgtcgtgg tgggtgacgc cgccaaacaa atggtgctgg
     5161 cggatattcg ttctattggt acgaatacta ttgatgtcta tcccgggaaa gattttggcg
     5221 atgacgatcc gcaatatcag caggcgctga agtacgacga cttaatcgcc atccaaaaac
     5281 aaccgtgggt cgcctcagcc acacctgccg tctcgcaaaa cctgcgcctg cgttataaca
     5341 atgttgatgt tgctgccagt gccaatggcg tgagcggcga ttattttaat gtctatggca
     5401 tgaccttcag tgaaggaaac acctttaatc aggagcagct gaacggtcgt gcgcaggtcg
     5461 tggttctcga cagtaatact cgccgccagc ttttccccca taaagcagat gtggttggcg
     5521 aggtgattct ggtcggcaat atgcccgcca gagtcattgg tgtggcggaa gaaaaacagt
     5581 cgatgtttgg tagcagtaaa gtgctgcgtg tctggctacc ttacagcacg atgtccgggc
     5641 gagttatggg ccagtcgtgg cttaactcca ttactgtcag ggtgaaagaa ggatttgaca
     5701 gcgccgaggc ggaacagcaa ctcacgcgtt tactttcact gcgccacggt aagaaggatt
     5761 tctttacctg gaacatggac ggcgtcttga aaactgttga aaagaccaca cgtactttac
     5821 aactgtttct gacgctggtg gcggtgattt cgctggtggt gggcggtatt ggtgtaatga
     5881 atattatgct ggtgtcagtg accgagcgga cgcgggaaat tggcattcgc atggctgtag
     5941 gtgcgcgagc aagcgatgtt ttgcaacagt tcctgatcga agccgtactg gtttgcctgg
     6001 tcggtggcgc gttgggaata acactgtcac tgttaattgc tttcaccttg cagcttttct
     6061 tacccggctg ggagattggt ttttcaccgt tggcgctgct gctggcgttt ctctgctcga
     6121 cggccaccgg gattttattt ggctggttac ccgcacgaaa tgcggcacga ctggatccag
     6181 tagatgctct ggcacgggag taatttttga gataaaaatg ccagccgatc gggctggcat
     6241 tttgccttta ggatgtacac aatgagacag aagagctatg cgactgccgc ttctacttcg
     6301 acgggcacaa taacactggc gtgattgcct tttggcccct ggtggacatc aaactgaacg
     6361 gattgtccag cttttagcgt tctgtaacca tccatctgaa tggtggaata atgagcgaaa
     6421 atatcttcgc cgccgccttc agggcagatg aaaccaaacc ctttggcatt gttgaaccac
     6481 ttaacagtac ccttttccat gcttcgacat ccttcgcaaa tcttatacaa gtaagatgga
     6541 ataaaccggg gtcagagagg gggctgttca aaacctcgcc aactctagaa atacaattta
     6601 gagaattagg gcgagccgtc aagcatttga caggggccag ggggcaggta tgaatcaaaa
     6661 atttgaagca gttaacgcta ttgacaggaa tgtgacagat gtcgctgatg ccaacgatag
     6721 atgatagtta tctatcatgt ggagtagatt ggtcaggcaa ataagctctt gtcagcggca
     6781 gggcgttctg ccgataaccg taaccgaaga tgataactga caatgggtaa aacgaacgac
     6841 tggctggact ttgatcaact ggcggaagaa aaagttcgcg acgcgctaaa accgccatct
     6901 atgtataaag tgatattagt caatgatgat tacactccga tggagtttgt tattgacgtg
     6961 ttacaaaaat tcttttctta tgatgtagaa cgtgcaacgc aattgatgct cgctgttcac
     7021 taccagggga aggccatttg cggagtcttt accgccgagg ttgcagaaac caaagtggcg
     7081 atggtgaaca agtacgcgag ggagaatgag catccattgc tgtgtacgct agaaaaagcc
     7141 tgaatgcagg cataaaaatt gggggaggtg cctatgctca atcaagaact ggaactcagt
     7201 ttaaatatgg ctttcgccag agcgcgcgag caccgtcatg agtttatgac cgtcgagcac
     7261 ttgttactgg cgctgctcag taacccatct gcccgggagg cgctggaagc gtgttctgtg
     7321 gatttggttg cgctccgtca ggaactggaa gcctttattg aacaaaccac acccgttctg
     7381 cctgccagtg aagaggagcg cgacacacag ccgacgctga gttttcagcg tgtactgcaa
     7441 cgtgcggtct tccatgtcca gtcctccggt cgcaatgagg ttaccggtgc aaacgttctg
     7501 gtcgctatct tcagcgaaca ggagtcgcag gcggcatatc tgttgcgtaa acatgaagtc
     7561 agccgtctcg atgtggtgaa ctttatctct catggcacgc gtaaagacga gccgacacag
     7621 tcttctgatc ctggcagcca gccaaacagc gaagaacaag ctggtgggga ggaacgtatg
     7681 gagaatttca cgacgaacct gaaccagctt gcgcgtgtgg gcggaatcga cccactaatt
     7741 ggtcgtgaga aagagctgga gcgtgctatt caggttctct gccgtcgccg taaaaacaac
     7801 ccgctgctgg tgggggaatc tggtgtcggt aaaaccgcga ttgcagaagg tcttgcctgg
     7861 cgaattgttc agggtgatgt gccggaagtg atggctgact gtacgattta ctctctcgat
     7921 atcggttctc tgttagcggg cacaaaatat cgcggcgact ttgaaaaacg ttttaaagcg
     7981 ttgctcaagc agctggagca ggacactaac agcatcctgt ttattgatga gatccacacc
     8041 attatcggtg cgggtgcagc gtctggtggt caggtcgatg cggctaacct aatcaaaccg
     8101 ttgctctcca gcggtaaaat tcgcgtaatt ggttcgacaa cctatcagga gttcagcaac
     8161 attttcgaga aagaccgtgc tctggcgcgt cgcttccaga aaattgatat tactgaaccg
     8221 tcgatcgaag aaactgttca aatcatcaat ggcctgaaac cgaagtatga agcgcaccac
     8281 gacgtgcgtt ataccgcaaa agcggtgcgt gcggcggtag agctggcggt gaaatacatt
     8341 aacgatcgtc atctgccgga taaagccatt gatgttatcg acgaagcagg cgctcgcgca
     8401 cgtctgatgc cggtaagcaa acgcaagaaa accgttaatg tggcggatat tgagtccgtg
     8461 gtggcccgta ttgcacgcat tccagagaag agtgtttctc agagtgaccg cgataccctg
     8521 aaaaaactca gcgatcgcct gaaaatgctg gtcttcggcc aggataaagc cattgaggcg
     8581 ctgactgaag ccattaagat ggcgcgtgca ggtttaggtc acgaacataa accggtcggt
     8641 tcgttcctgt ttgccggtcc taccggggtc gggaaaacag aggtgacggt acagctttcg
     8701 aaagcgttgg gcattgagct tctgcgcttt gatatgtccg agtatatgga acgccatacc
     8761 gtcagccgtc tgattggtgc gcctccggga tacgttggtt ttgatcaggg cggtttgctg
     8821 accgatgcgg taatcaagca tccacatgcg gtgctgctgc tggacgaaat cgagaaagcg
     8881 catccggacg tgttcaatat tctgttgcag gtgatggaca acggtacgct gaccgataac
     8941 aacggacgca aagcggactt ccgtaacgtg gtgctggtga tgaccactaa cgccggggta
     9001 cgggaaactg agcgcaaatc cattggtctt atccaccagg ataacagcac cgatgcgatg
     9061 gaagagatca agaagatctt tacaccggaa ttccgtaacc gtctcgacaa cattatctgg
     9121 ttcgatcatc tgtcaactga cgtgatccat caggtggtgg ataaattcat cgtcgagttg
     9181 caggttcagc tggatcagaa aggtgtttct ctggaagtga gccaggaagc gcgtaactgg
     9241 ctggccgaga aaggttacga ccgggcaatg ggcgcacgtc cgatggcgcg tgtcatccag
     9301 gacaacctga aaaaaccgct cgccaacgaa ctgctgtttg gttcgctggt ggacggcggt
     9361 caggtgacgg ttgcgctgga taaagagaaa aatgagctga cttacggatt ccagagtgca
     9421 caaaagcaca aggcggaagc agcgcattaa tctgattgtc aggtaggttg gtgaagtccg
     9481 taatctcgtc aggggttacg gactttttat ttatggggtg gaacggtgaa aaccctattt
     9541 ttggaggtga aggtaagttg ttgataatta gtgctgctgg aaggtaagga taaaaaaggg
     9601 tgctgcagga gaatgggatg gttttgcttt attaacaacg ggctaaacgt gtagtatttg
     9661 agttcactgc cgtacaggca gcttagaaat cgacggggtg cggtaaaacc tttgcgaacg
     9721 cgttcactgc cgtacaggca gcttagaaat tcacaggtaa catactccac ccgcccacca
     9781 tgttcactgc cgtacagaca gataaaatgc gaaaaaaaag ctcgcacttt cgtacgagct
     9841 cttctttaaa tatggcggtg agggggggat tcgaaccccc gatacgttgc cgtatacaca
     9901 ctttccaggc gtgctccttc agccactcgg acacctcacc aaattgtttt gctaccaaac
     9961 ctcatgggtg gcaacggggc gctactatag ggagttggag taaaacggtc aagaagaatt
    10021 ttaatgataa ttattgtttg ctcatactgt aaacaacttg tgcagtatat ctacatcgag
    10081 acaagttatg gacttatact tccaaagtac ttcatacata tcacaaaata aaaaggccgg
    10141 ttaaaccgac cttttactcg ttctttctct tcgcccatca ggcggtaaaa caatcagcga
    10201 ctacggaaga caatgcggcc tttgctcagg tcgtacgggg tcagttcaac agtcactttg
    10261 tcgcccgtca ggatgcggat gtagtttttg cgcattttac cggagatgtg tgcagtaacc
    10321 acgtgaccgt tttctaactc tacgcggaac atggtattag gcaacgtttc aagaacggta
    10381 ccttgcattt caatattgtc ttctttggcc atctaatcct ctggggtatc actaccgtaa
    10441 tttgaaccgg caagataatg ccgaagttct gtaaataagt aaagatttgc gcgctaaatc
    10501 gcaacaaaca ggttcggcac attactccga aaacacacgg ctaagccgca ccaaaagcgc
    10561 aacgtataag ggagcggtga gataaacgat gggcgttacc tgacgcgaaa aattccttat
    10621 cggcagcggg gtaatgagcg taaccaactc tgcgaccgca attataacac tctggggaga
    10681 aatgtgccga aaacattcat tcttgtggtg aaaacaagca ccgtggtacc cagaaattat
    10741 tcggcaatcg tccgaggcgc atttgattga gataattaag gtaatcccgg cggggaattt
    10801 cgcaggcacc aagcgatgct gtgtgatcgt taaggacctg gcagtcgata agcttaccgc
    10861 catgaccgat aaattcctca cagaatacca gaagcgccgt tttagacgca ttttccatcc
    10921 ggctgaacat ggactcgcca caaaatagcg ttccctgggc cacgccgtac ataccgccga
    10981 caagctcatc ttcacgccag acttcaatgg agtgggcatg accgagttcg tgaaggcgat
    11041 ggtaggcttc gaccacgcca cgcgtgatcc aggttccttc ttcgcgatcg ctggcacagc
    11101 cttcaatgac ctgaccaaaa gcgtaattca tcgtgacacg atagggcgag cgtttatgaa
    11161 atcgcttcat actacggctg atatgcagtg attctggcca tagcaccgcg cggggatcgg
    11221 gcgaccacca gaggatgggg tcgcctggag aaaaccacgg aaaaatacca cgctggtaag
    11281 ccattaacag gcgcgcaggg ctaagatcgc ccccaagtgc cagcaggccg ttaggctcac
    11341 gtaatgcgcc ttccggggaa gggaaggcta ttgaatggcg agaaagctga accaagcgca
    11401 tgaccgcaaa actccacgca agtcggatcg ttcaataata gcttacaaac cctgcttgaa
    11461 ctggtaataa cgcccctgtc tggcaagcag ttctgcgtga gtaccttgct caataatttg
    11521 cccgttgtcc atcactatta tttgttggaa acgagagagt ccgcgaagtc gatgggtgac
    11581 cattaacacc gttttctcac gcatcatttt tgcaagcaat tcaaggatct ggctttcggt
    11641 tgtggcatct aagccttcgg taggttcatc cagcaacacc agtggcgcat catgtaacag
    11701 cgtacgggcg atagccagac ggcgcagttc accaccggag agctggcgtc cgccttcacc
    11761 taaccaactg ttgagacctg catcctcgag cagcttctcc agaccaacgc gacgcaagat
    11821 ctccgacaga gcctcatcac tactgccagg cgaggcgagt aacagattat cacgcagtgt
    11881 ggcgctaaac agatgcactc gctgaggaac aacgctgatg gtctgtcgta gagccgcttc
    11941 attcaggctg gctatggggc tatcgttaag caaaatctcg ccctgttgcg ggtcccatgc
    12001 gcgggtcagc agttgtaaca gtgttgattt gccgcatccg gttcgcccga gaatcgctat
    12061 atgttccccg gcgtttacct gaagagaaat ccctttaagt gcctgttggg attgctccgg
    12121 ataagtgaac tgaacatccc gtaatgtcag cgaaacgcga tcggcaacac gagtttgggt
    12181 atcagggaag gtgacctccg gtttttgatc cgttaagtca gagatacgta cggcagaggc
    12241 aatgacttgc cccagatgct gaaatgcacc cgttactggt gccagtgctt caaacgcggc
    12301 taacgcacaa aagacaaaca gggcaattaa tgcgccgggt tgagcattgc cgccaacgcc
    12361 gccagacgcc atccacagca tcaggatcac cgctaacgcg ccaatgagca gcattatcgc
    12421 ttgcgacaat gcggtcagtt cagattgacg gcgttgcgct tccagccatt gaatttctgt
    12481 attctctagt tgcgtgcgat aacgatcgct ggcaccaaaa atggtcagct cagcttgccc
    12541 ttgcagccag gccgtcagtt gttggcgata ctgtccgcga agatgagtca gattttgccc
    12601 ggtgcttttt cccgcacgat aaaacagcgg tggcatcagg aaaagcgtca gtaacataac
    12661 gctgcccagc gtaaaggcga gggtgaaatc aaggaaactt aacccgattg tcaccaccat
    12721 aatcaccaca aaagcgccca ccagcggcga gataacgcgc aggtaaagat gatcgagcgt
    12781 atcaacatcc gccaccacgc ggttgagcaa ttcgccctga cgatagcgcg ccagtccggc
    12841 aggggagagg ggcaacaatt tgctgaaggt gtaaatgcgc agatgctgca acacgcggaa
    12901 agtcgcgtcg tgacttacca gacgttcaaa ataacgcccg gcagtacggg tgattgcggc
    12961 accacgcacg cccgcagcgg gtagcatata gttgaagctg tacagtccgg caacccccgc
    13021 aaccgctgag gccgagagga accagccgga aagtgtcaac agaccgatac tggcgagcag
    13081 cgtcacaatt gccagcacaa taccaagact taacatccat ttatgacgtt tatacagtgc
    13141 cagatagggt agcaaagcgc gcatttaaat ctcctcctga cgatgggcca gtaatgtggc
    13201 gaatgggcca ccagccacac ttaattccgc gtaacgtcct tgctcaataa tccggccatc
    13261 ctgcataacc caaatgacat cccagtcagc aagatcttct aactggtggg tgaccattaa
    13321 cgttgtctgg cgcagagagg cggcattcag cgcctccatt acgcgctgtt cactgtgagc
    13381 atcaaggctg gcagcgggtt catccaacag taatagcgaa cagggattta gtaacgcacg
    13441 ggccaccgcc acgcgctgcg cctgccccac ggaaaggcgg gcagcctggt cgccaacagg
    13501 tgtatcaacg ccttgtggca ggagcggtag aaactcgctg acccaggcgt tatccagcgc
    13561 tgcttgtaat tcttgttcgc tggcatcagg tcgcgccagt agtacgttat cccgcaattt
    13621 tgctgccggt aattgtgggt tttgcccaac ccaggagaga tgtttacgcc atgattctgg
    13681 tgataaatca cgtaattcta tcccgttgat tcgtaatgat ccctgatatg agagaaaacc
    13741 agaaagcgcg ttcagcagtg agcttttacc tgaaccgctg cggccaacca acactgcacg
    13801 ttggcctgct ggcaaagtaa agttcagcgg tccggccagc gttttacctt ccggcgacgt
    13861 gataaacaga tcctcggcct caatggtcag cggatcggtc aatgctaatt ccgcctcacc
    13921 gcgctgcgga tgggcgagcg gggtttccat aaacgttttc agactgtcag ctgcaccaac
    13981 agcctgggct ttagcatgat aaaacgtacc gagatcgcgt aatggctgga aaaactctgg
    14041 cgcaaggatc agggccagaa aacctgcagc cagcgtcacc ccagtatcgt agtgaccaaa
    14101 atccagctcg ccgagatagg aaaaaccaaa gtagaccgcc accagagcaa ttgacagcga
    14161 ggtaaaaaat tcgagaatgc cggaggataa aaacgccagt cgtagcactt ccattgtccg
    14221 ttggcggaaa tcttccgaag cagaacgaat actttcaatt tcagcttcac cacgaccaaa
    14281 aatacgcaat gtttccatgc cgcgcaggcg atcgaggaaa tgcccactta agcgagcaag
    14341 agcgagaaag ttacgccggt tagcatcggc agcccccatt ccaaccagcg ccataaacaa
    14401 cggaattagc ggtgctgtgc ccagcagaat gagcgccgca gcccagttag aggggaagat
    14461 cgccaccaca atcagcaacg gcaccgacac tgccagcgcc atttgcggca gatagcgtgc
    14521 atagtaatca tgcatatcgt caatttgctc gagtaccagc gtcgcccagc tccccgcagg
    14581 tttaccctga atccacgctg gccctgcttg ttgcagacgg tcgagaacct gacggcggat
    14641 ggcaaagcgg atatgctgcc cggcgtgata acccacccgt tcgcgtaacc agaccaccca
    14701 tgcgcgcaat acaaaggtca gaaccagtaa cgtaaaggga agcagcaggg cttcacgggg
    14761 aatattctcc ataatcatat gttgcagaat tcgcgccatg aaccaggcct gggcaatgat
    14821 caatatgccg cttacaaagc ccagcagacg agaaatattc agccaacgtt gggagatgac
    14881 gctttgctgt tttaaccagc gggttaactc tttttgacga gatttattca ttgcacgctt
    14941 agcaggtgag ttatcagaat tatttgcaga gcaatgttac aacggggaaa aaataaaggc
    15001 gacccatagt cgcaaggtgt cgccttcttt acttttgtta ctgatttgta aaattatttt
    15061 gcgtcagcta aaccatcgag gtagcgttcc gcatcaagtg ctgccatgca gccagtaccg
    15121 gccgaagtaa tggcctggcg ataaatgtga tccatcacgt cgcctgcggc aaagacgcca
    15181 ggaatgctgg tctgggtggc attaccatga atacccgact gtactttgat gtagccgttt
    15241 tccagttcca gctgcccttc gaaaatcgca gtattcgggc tgtgaccgat agcaacaaac
    15301 agaccggcaa cgtcgagtga ctcgatgtta tcgctgtttt gcgtatcgcg cagacgaacg
    15361 ccagtgacac ccatttgatc gccggtcact tcttccagcg tacggttggt gtgcagaatg
    15421 atgttgccgt tctccacttt atccatcaga cgcttaataa ggattttttc cgcgcggaaa
    15481 ccgtcacggc ggtgaatcag atgcacttcc gaagcgatgt tagacagata cagcgcctct
    15541 tcaaccgcgg tattgccgcc gccgatgacc gcaactttct ggttgcgata gaagaaaccg
    15601 tcgcaggttg cacaagcaga aaccccacgg cctttaaagg cttcttcaga gggcaggccg
    15661 agatagcgtg cagaagctcc ggtggcaata atcagcgcgt cgcaagtgta ttcgccgtta
    15721 tcgccattca gacggaacgg acggttttgc agatccacct tgttgatatg atcaaaaatg
    15781 atctcagttt caaacttggt ggcatgttcg tgcatgcgcg ccattaataa cggaccggtc
    15841 agatcgtttg gatcaccagg ccagttttcc acttccgtgg tggtggtcag ttggccgcct
    15901 ttttccatgc cggtaatcag cacaggttgc aggttggcgc gcgccgcgta gacagcagcg
    15961 gtgtatcccg ccgggcctga acccaggata agcagtttac tgtgtttggt cgtgcccatg
    16021 agatccccat agttgttggc agacaatggg caggattgta gggaatttac agacgtaaaa
    16081 aaagagtatg acgattttgt taacaatttg tgcaatcggc agcatcgata agcaggtcaa
    16141 attctcccgt cattatcacc tctgctactt aaatttcccg ctttataagc cgattaaatg
    16201 atgaataaac gcccctgtta atgaatatct ggcatgttgt actaaaaatc gatgttttgc
    16261 tttgacaatc ccctggtgtt ttgcgaaaac attcgaggaa gaaaaaaaca gtattcttat
    16321 atgcgcataa ccatgtatgt aaataccatg tttaccgtgc tagtgaaatc tacgtatggc
    16381 gtggacagac gccattcgtg atgtcgatag ctgccacaag gcaacggtct tctcaccgta
    16441 gacccaggca ttgcgcgccg tgaatcttca tgatttcggt ctatcgtgac gggtagcgac
    16501 tctgaacagt gatgtttcag ggtcagacag gagtagggaa ggaatacaga gagacaataa
    16561 taatggtaga tagcaagaag cgccctggca aagatctcga ccgtatcgat cgtaacattc
    16621 ttaatgagtt gcaaaaggat gggcgtattt ctaacgtcga gctttctaaa cgtgtgggac
    16681 tttccccaac gccgtgcctt gagcgtgtgc gtcggctgga aagacaaggg tttattcagg
    16741 gctatacggc gctgcttaac ccccattatc tggatgcatc acttctggta ttcgttgaga
    16801 ttactctgaa tcgtggcgca ccggatgtgt ttgaacaatt caataccgct gtacaaaaac
    16861 ttgaagaaat tcaggagtgt catttagtat ctggtgattt cgactacctg ttgaaaacac
    16921 gcgtgccgga tatgtcagct taccgtaagt tgctggggga aaccctgctg cgtctgcctg
    16981 gcgtcaatga cacacggaca tacgttgtta tggaagaagt caagcagagt aatcgtctgg
    17041 ttattaagac gcgctaacac ggaacaggtg caaaatcggc gtattttgat tacactcctg
    17101 ttaatccata cagcaacagt actggggtaa cctggtactg ttgtccgttt tagcatcggg
    17161 caggaaaagc ctgtaacctg gagagccttt cttgagccag gaatacactg aagacaaaga
    17221 agtcacattg acaaagttaa gtagcggccg ccgccttctg gaggcattgc tgatccttat
    17281 tgtcctgttt gccgtctggt tgatggctgc cttactaagc tttaaccctt cggaccccag
    17341 ctggtcgcaa acggcctggc atgaacctat ccataattta ggtgggatgc ccggtgcgtg
    17401 gttggcagat acgctgttct ttatttttgg cgtgatggct tacaccattc ccgtcattat
    17461 tgtcggcggt tgttggtttg cctggcgtca tcagtccagc gacgaataca ttgattattt
    17521 tgccgtttcg ctacgcatca ttggcgtttt ggcgctcatc cttacctcct gtggtctggc
    17581 ggcaatcaac gctgacgata tctggtattt tgcctccggt ggcgtcattg gcagcttact
    17641 aagcactacg ctacaaccac tgctacacag tagcggggga actattgcgc tgctctgcgt
    17701 ttgggcagcg ggcctgacgt tgttcaccgg ttggtcatgg gtgaccattg ctgaaaaact
    17761 cggcggctgg attttaaaca ttctcacctt cgccagtaat cgtacccgtc gcgatgatac
    17821 ctgggtcgat gaagatgagt atgaagacga cgaagagtat gaagatgaaa atcacggcaa
    17881 acagcatgaa tcacgccgtg cccgtattct tcgcggcgcg ctagcgcgtc gtaaacggtt
    17941 ggcggaaaaa ttcattaatc cgatggggcg gcaaacagac gctgcgttgt tctccggtaa
    18001 gcggatggat gatgacgaag agattaccta cactgcacgc ggtgtggctg ccgacccgga
    18061 cgacgtccta ttttcgggca atcgtgcaac gcagccagaa tatgacgaat acgatccatt
    18121 attaaacagt gcgccaatta ccgaacctgt cgctgtagca gctgctgcta ccacggcgac
    18181 acaaagctgg gctgcgccgg ttgaacctgt gactcagacg ccgcctgttg cctctgttga
    18241 tgttccacct tcgcaaccta cagtagcctg gcagcctgta ccgggtccac aaacgggaga
    18301 gccggttatt gctcctgcac cggaaggtta cccacagcag tcacaatatg cgcagcctgc
    18361 agtgcaatat aatgagccgc tgcaacaacc agtacagccg cagcagccgt attatgcacc
    18421 tgcagctgaa caacctgcgc aacagccgta ttatgcccct gcgccagaac aaccggtggc
    18481 aggtaacgcc tggcaagccg aagagcagca atccactttt gctccacagt ctacatacca
    18541 gactgagcaa acttatcagc agccagccgc tcaggagccg ttgtaccaac agccgcaacc
    18601 cgttgaacag cagcctgttg tggagcctga acccgttgta gaagagacaa aacccgcgcg
    18661 tccgccgctt tactattttg aagaagtgga agagaagcga gcccgtgaac gtgaacaact
    18721 tgcggcctgg tatcaaccga ttccagaacc ggttaaagaa ccagaaccga tcaaatcttc
    18781 gctgaaagca ccttctgttg cagcagtacc tccagtagaa gccgctgccg ctgtttctcc
    18841 gctggcatct ggcgtgaaaa aagcgacact ggcgacgggg gctgccgcaa ccgttgccgc
    18901 gccagtcttc agtctggcaa atagcggtgg accgcgtccg caggtcaaag aggggattgg
    18961 cccgcagttg ccacgaccga aacgtatccg cgtgccaact cgtcgtgaat tggcgtctta
    19021 cggtattaag ctgccctcac agcgtgcggc ggaagaaaaa gcccgtgaag cccagcgcaa
    19081 tcagtatgat tctggcgatc agtataacga tgatgaaatc gatgcgatgc agcaggatga
    19141 actggcacgt cagttcgccc agacacagca gcaacgctat ggcgaacagt atcaacatga
    19201 tgtgcccgta aacgcagaag atgcagatgc tgcggcagag gctgaactgg ctcgtcagtt
    19261 tgcgcaaact caacaacaac gttattccgg cgaacaaccg gctggggcga atccgttctc
    19321 gctggatgat tttgaatttt cgccaatgaa agcgttgctg gatgatggtc cacacgaacc
    19381 gctgtttacg ccaattgttg aacctgtaca gcagccgcaa caaccggttg caccacagca
    19441 gcagtatcag cagccacaac agccggttgc gccacagcag cagtatcagc agccacaaca
    19501 gcaggttgcg ccgcagccac aatatcagca gccgcaacaa ccggttgcgc cacagcagca
    19561 atatcagcag ccgcaacaac cagttgcgcc gcagccgcag tatcaacagc cgcaacaacc
    19621 ggttgcaccg cagccgcagt atcagcagcc acaacagcca gttgcgccac agccgcagga
    19681 taccctgctt catccgctgt tgatgcgtaa tggcgacagc cgtccgttgc acaaaccgac
    19741 gacgccactg ccttctctgg atttgctgac accgccgccg agcgaagtgg agccggtaga
    19801 tacctttgcg cttgaacaaa tggcgcgtct ggtggaagcg cgtctggctg atttccgtat
    19861 taaagccgat gtcgttaatt actctccggg gccagttatc actcgctttg aacttaacct
    19921 ggctcctggc gtaaaagcgg cgcgcatttc taacttgtca cgggaccttg cccgttcact
    19981 ttcgacggtg gcggtgcgtg tcgttgaagt tattcctggc aaaccctatg taggtctgga
    20041 gttaccaaat aaaaaacgac aaaccgttta tctgcgcgaa gttttggata acgccaaatt
    20101 ccgcgataat ccgtcgccat taaccgtggt gctgggtaaa gatatcgccg gtgagccggt
    20161 ggttgccgat ctggcgaaaa tgccgcactt gttggttgcg gggactaccg gttccggtaa
    20221 atctgtcggt gtgaacgcga tgatcctgag catgctttat aaagcacagc cagaagatgt
    20281 gcgtttcatc atgatcgacc cgaaaatgct ggagctttcg gtttatgaag gcattccgca
    20341 cctgttaacg gaagtcgtta ctgatatgaa agatgccgcc aacgcgctgc gctggtgtgt
    20401 taacgagatg gagcgtcggt ataaactgat gtctgcgctg ggtgtgcgta atctggcggg
    20461 ttataacgaa aaaattgctg aagccgatcg catgatgcgt ccgattccag acccgtactg
    20521 gaagccgggt gacagtatgg atgcccagca tccggtgctg aaaaaagaac catacattgt
    20581 cgtgttggtt gacgaatttg ccgacctgat gatgacggta ggtaaaaaag tggaagagct
    20641 gatagctcgt ctggcgcaaa aagcccgtgc cgcgggtatc cacctcgtac tggcaactca
    20701 gcgtccatcg gttgatgtta ttactggtct gattaaagcg aatattccga cccgtatcgc
    20761 ctttaccgta tccagtaaga ttgactcacg taccattctt gatcaggctg gcgcggaatc
    20821 actgctgggt atgggggata tgctctactc tgggccgaac tccacgttgc cggtacgtgt
    20881 ccatggtgct tttgttcgcg atcaggaagt tcatgccgtg gtgcaggact ggaaagcgcg
    20941 tggtcgccca cagtatgttg atggcatcac ctccgacagc gaaagcgaag gtggtgcggg
    21001 tggtttcgat ggcgctgaag aactggatcc gttgttcgat caggcggtgc agtttgtcac
    21061 tgaaaaacgc aaagcgtcaa tttctggcgt acagcgtcag ttccgcattg gttataaccg
    21121 tgcagcgcgt attatcgaac agatggaagc gcaggggatt gtcagcgaac aggggcacaa
    21181 cggtaatcgt gaagtgctgg ccccaccgcc gtttgactaa ttaatgcatt gccggataag
    21241 gcgcggtagc gtcgcatccg gcactctatc aactgaaaat tcagtatttt cttctttcct
    21301 caagctgatt attagcctgg aatagagagt agagggaact cccgatcggg agtgacgtaa
    21361 tttgaggaat aatgatgaaa aaaattgcta tcacctgtgc attactctca agcttagtag
    21421 caagcagcgt ttgggctgat gccgcaagcg atctgaaaag ccgcctggat aaagtcagca
    21481 gcttccacgc cagcttcaca caaaaagtga ctgacggtag cggcgcggcg gtgcaggaag
    21541 gtcagggcga tctgtgggtg aaacgtccaa acttattcaa ctggcatatg acacaacctg
    21601 atgaaagcat tctggtttct gacggtaaaa cactgtggtt ctataacccg ttcgttgagc
    21661 aagctacggc aacctggctg aaagatgcca ccggtaatac gccgtttatg ctgattgccc
    21721 gcaaccagtc cagcgactgg cagcagtaca atatcaaaca gaatggcgat gactttgtcc
    21781 tgacgccgaa agccagcaat ggcaatctga agcagttcac cattaacgtg ggacgtgatg
    21841 gcacaatcca tcagtttagc gcggtggagc aggacgatca gcgcagcagt tatcagctga
    21901 aatcccagca aaatggggct gtggatgcag cgaaatttac cttcaccccg ccgcaaggcg
    21961 tcacggtaga tgatcaacgt aagtagaggc acctgagtga gcaatctgtc gctcgatttt
    22021 tcggataata cttttcaacc tctggccgcg cgtatgcggc cagaaaattt agcacagtat
    22081 atcggtcagc aacatttgct ggctgcgggg aagccgttgc cgcgcgctat cgaagccggg
    22141 catttgcatt ctatgatcct ctgggggcca ccgggtaccg gcaaaacaac cctcgctgaa
    22201 gtgattgccc gctatgcgaa cgctgatgtg gaacgtattt ctgccgtcac ctctggcgtg
    22261 aaagagattc gcgaggcgat cgagcgcgcc cggcaaaacc gcaatgcagg tcgccgcact
    22321 attctttttg ttgacgaagt tcaccgtttc aacaaaagcc agcaggatgc atttctgcca
    22381 catattgaag acggcaccat cacttttatt ggcgcaacca ctgaaaaccc gtcgtttgag
    22441 cttaattcgg cactgctttc ccgtgcccgt gtttatctgt tgaaatccct gagtacagag
    22501 gatattgagc aagtactaac tcaggcgatg gaagacaaaa cccgtggcta tggtggtcag
    22561 gatattgttc tgccagatga tacacgacgt gccattgcag aactggtgaa tggcgacgcg
    22621 cgccgggcgt taaatacgct ggaaatgatg gcggatatgg ccgaagtcta tgatagcggt
    22681 aagcgggtcc tgaagcctga attactgacc gaaatcgccg gtgaacgtag cgcccgcttt
    22741 gataacaaag gcgatcgctt ttacgatcta atttccgcac tgcataagtc ggtacgtggt
    22801 agcgcacccg atgcggcgct gtactggtat gcgcgaatta ttaccgctgg tggcgatccg
    22861 ttatatgtcg cgcgtcgctg tctggcgatt gcgtctgaag acgtcggtaa tgccgatcca
    22921 cgggcgatgc aggtggcaat tgcggcctgg gattgcttta ctcgcgttgg cccggcggaa
    22981 ggtgaacgcg ccattgctca ggcgattgtt tacctggcct gcgcgccaaa aagcaacgct
    23041 gtctacactg cgtttaaagc cgcgctggcc gatgctcgcg aacgcccgga ttatgacgtg
    23101 ccggttcatt tgcgtaatgc gccgacgaaa ttaatgaagg aaatgggcta cgggcaggaa
    23161 tatcgttatg ctcatgatga agcaaacgct tatgctgccg gtgaggttta cttcccgccg
    23221 gaaatagcac aaacacgcta ttatttcccg acaaacaggg gccttgaagg caagattggt
    23281 gaaaagctcg cctggctggc tgaacaggat caaaatagcc ccataaaacg ctaccgttaa
    23341 tgttatcgtt gcggtaatgt tgttactgta tccctgtggt cgcaggctgt ggccacatct
    23401 cccatttaat tcgataagca caggataagc atgctcgatc ccaatctgct gcgtaatgag
    23461 ccagacgcag tcgctgaaaa actggcacgc cggggcttta agctggatgt agataagctg
    23521 ggcgctcttg aagagcgtcg taaagtattg caggtcaaaa cggaaaacct gcaagcggag
    23581 cgtaactccc gatcgaaatc cattggccag gcgaaagcgc gcggggaaga tatcgagcct
    23641 ttacgtctgg aagtgaacaa actgggcgaa gagctggatg cagcaaaagc cgagctggat
    23701 gctttacagg ctgaaattcg cgatatcgcg ctgaccatcc ctaacctgcc tgcagatgaa
    23761 gtgccggtag gtaaagacga aaatgacaac gttgaagtca gccgctgggg taccccgcgt
    23821 gagtttgact ttgaagttcg tgaccacgtg acgctgggtg aaatgcactc tggcctcgac
    23881 tttgcagctg cagttaagct gactggttcc cgctttgtgg taatgaaagg gcagattgct
    23941 cgcatgcacc gcgcactgtc gcagtttatg ctggatctgc ataccgaaca gcatggctac
    24001 agtgagaact atgttccgta cctggttaac caggacacgc tgtacggtac gggtcagctg
    24061 ccgaaatttg ctggcgatct gttccatact cgtccgctgg aagaagaagc agacaccagt
    24121 aactatgcgc tgatcccaac ggcagaagtt ccgctgacta acctggtgcg cggtgaaatc
    24181 atcgatgaag atgatctgcc aattaagatg accgcccaca ccccatgctt ccgttctgaa
    24241 gccggttcat atggtcgtga cacccgtggt ctgatccgta tgcaccagtt cgacaaagtt
    24301 gaaatggtgc agatcgtgcg cccagaagac tcaatggcgg cgctggaaga gatgactggt
    24361 catgcagaaa aagtcctgca gttgctgggc ctgccgtacc gtaaaatcat cctttgcact
    24421 ggcgacatgg gctttggcgc ttgcaaaact tacgacctgg aagtatggat cccggcacag
    24481 aacacctacc gtgagatctc ttcctgctcc aacgtttggg atttccaggc acgtcgtatg
    24541 caggcacgtt gccgcagcaa gtcggacaag aaaacccgtc tggttcatac cctgaacggt
    24601 tctggtctgg ctgttggtcg tacgctggtt gcagtaatgg aaaactatca gcaggctgat
    24661 ggtcgtattg aagtaccaga agttctgcgt ccgtatatga acggactgga atatattggc
    24721 taatacccaa tttttctgaa tctaaaaagc gcctttaggg cgcttttttt gtctcccttt
    24781 gataccgaac aataattact cctcacttac acgtaatact actttcgagt gaaaatctac
    24841 ctatctcttt gattttcaaa ttattctatg tatacaagcc tatatagcga gctgctatag
    24901 aaataattac acaatacggt ttgttactgg aatcaatcgt gatcaagctt gagtgagcca
    24961 ttatgaaaac gaaaatccct gatgcggtat tggctgctga ggagagtcgc cgtggtttgg
    25021 taaaaacgac agcgatcggc ggcctggcaa tggccagcag cgcattaaca ttacctttta
    25081 gtcggattgc gcacgctgtc gatagcgcca ttccaacaaa atcagacgaa aaggttatct
    25141 ggagcgcctg tacagttaac tgtggtagtc gctgcccgct acgtatgcac gtcgtggacg
    25201 gtgaaatcaa atatgtcgaa acgacaatac cggcgatgac aattacgacg gcctacacta
    25261 ggttcgcgcc tgcctgcgtg ggcgttccat gcgtcaccgt gtctacaatc cggaccgcct
    25321 gaaatatccg atgaaacgag tcggggcgcg cggtgaaggc aaattcgagc gcattagctg
    25381 ggaagaagcc tacgacatca tcgcgaccaa tatgcagcgc ctgatcaaag agtacggcaa
    25441 cgagtctatc tatctgaact atggcaccgg tacgctgggc ggcaccatga cccgctcctg
    25501 gccgccggga aataccctgg tcgcgcgcct gatgaactgc tgcggcggtt atcttaacca
    25561 ttacggtgac tactcttccg cgcaaattgc ggaaggtttg aaccatacct acggcggctg
    25621 ggcagatggt aacagcccgt cggatatcga aaacagtaaa ttggtagtgc tgtttggtaa
    25681 taacccaggc gaaacgcgaa tgagtggcgg tggggtgact tactatcttg aacaggcacg
    25741 ccagaaatct aatgctcgca tgatcatcat cgatccgcgc tataccgaca ccggtgccgg
    25801 gcgcgaagat gagtggatcc ctattcgtcc aggaacagat gccgcactgg ttaacggtct
    25861 ggcgtacgtc atgatcacta aaaacctggt ggatcaggca ttcctcgata aatattgcgt
    25921 tggctatgac gagaaaaccc tgcccgccag tgcgccgaaa aatggtcatt ataaagctta
    25981 tattctgggt gaagggccag atggcgtggc taaaacaccg gaatgggcct cgcaaatcac
    26041 cggtgttccg gcagacaaaa tcatcaaact ggctcgtgaa atcggcagta ccaaaccggc
    26101 gtttatcagc cagggatggg gcccgcagcg tcacgctaac ggtgaaatcg caacccgtgc
    26161 tatctcgatg ctggcgattc tgaccggtaa cgttggtatt aatggaggga acagcggcgc
    26221 gcgtgaaggt tcatacagct taccgtttgt ccgtatgccg accttggaaa acccgatcca
    26281 gaccagcatt tcgatgttta tgtggaccga tgccattgaa cgtggcccgg aaatgacggc
    26341 gctgcgtgat ggtgtgcgcg ggaaagataa gctggatgtg ccgatcaaaa tgatctggaa
    26401 ctatgccggt aactgcctga ttaaccagca ttctgaaatc aaccgtaccc atgaaatcct
    26461 ccaggatgat aagaagtgcg agctgattgt ggttatcgac tgccacatga cctcatcggc
    26521 gaaatatgct gacatcctgc tgcctgactg caccgcttcc gaacagatgg actttgcact
    26581 ggatgcatcc tgcgggaata tgtcttacgt gattttcaac gatcaggtga ttaaaccgcg
    26641 ctttgaatgt aagaccatct atgaaatgac cagcgaactg gcaaaacgtc ttggcgttga
    26701 gcaacagttt actgaaggcc gtacccagga agagtggatg cggcatctgt atgcccagtc
    26761 gcgggaagcg attcctgaac tgccaacgtt tgaagagttc cgcaagcagg ggatctttaa
    26821 aaagcgcgac ccacaagggc atcacgttgc ttataaagcc ttccgtgaag atccgcaggc
    26881 aaacccactg actacgccat cgggcaaaat tgagatttat tcgcaggcgc tggctgacat
    26941 tgccgctacc tgggaattgc cagaaggcga tgtgatcgat ccactgccga tctacacgcc
    27001 gggctttgaa agttatcagg atccgctgaa caaacagtat ccgctgcagc ttacaggttt
    27061 ccactataaa tctcgcgttc actcaactta cggcaacgtt gatgtgctga aagcggcttg
    27121 ccgtcaggaa atgtggatca acccgcttga tgcccaaaaa cgcggtatcc acaacggcga
    27181 taaagtcagg atctttaacg atcgtggtga ggttcatatt gaggcgaaag tgacgccacg
    27241 aatgatgccg ggcgtggtcg cactgggtga aggcgcctgg tatgacccgg atgcaaaacg
    27301 tgtcgataag ggcggttgta tcaacgtact gaccactcaa cgtccgtctc ctctcgctaa
    27361 ggggaatccg tcacatacaa accttgttca ggttgaaaag gtgtaaggag taaccgatga
    27421 caacccagta tggatttttt attgattcca gccgttgcac cggttgcaaa acctgcgagc
    27481 tggcttgtaa agactacaaa gatttgacgc cagaagtcag tttccgccgc atttatgaat
    27541 atgctggcgg cgactggcag gaagataacg gtgtctggca ccagaacgtg tttgcctact
    27601 atctgtcgat ttcgtgcaac cactgcgaag atccggcttg tactaaagtc tgcccgagcg
    27661 gtgcgatgca taaacgcgaa gatggttttg ttgtggtcga tgaagatgta tgcattggct
    27721 gccgttactg tcatatggct tgcccgtatg gcgcaccgca atataacgaa acgaaaggcc
    27781 atatgaccaa atgcgatggt tgttatgacc gtgttgcgga gggtaaaaag ccgatctgtg
    27841 ttgaatcttg cccgctgcgg gcgctggatt tcggccctat cgacgagctg cgtaaaaaac
    27901 atggcgatct ggcggccgtt gcgccgttgc cgcgagctca ctttaccaaa ccgaatattg
    27961 tgatcaaacc caatgccaat agccgcccga ccggggatac cactggctat ctggcaaacc
    28021 cgaaggaggt gtgagatggg aagtggatgg catgaatggc cgctgatgat cttcacggtc
    28081 ttcgggcaat gtgtggcggg tggttttatc gttctggctt tggcgctgct caaaggcgac
    28141 ctgcgagcag aagcccagca gcgtgttatc gcctgcatgt ttggcttatg ggtactgatg
    28201 ggcattggct ttatcgcctc tatgctccat cttggttcac cgatgcgcgc ctttaactcg
    28261 ctcaaccggg taggggcttc agcactcagt aacgaaatcg ccagcggttc gatctttttt
    28321 gctgtaggcg gcatcggctg gttgctggca atgctgaaaa agctgtcacc ggcattgcgt
    28381 acgctatggc tggtggtgac gatggttctt ggcgtcgtct ttgtctggat gatggtgcgt
    28441 gtgtataaca gcattgatac cgttccgacc tggtacagca tctggacgcc gatgggctgg
    28501 gcgatgcgtc tgctgccagc catttctgta ctggcactgg tggtgagtgg cgtgatgtca
    28561 gtgatgcagg gcacagagct ggcaactatt catagttctg tgcagcaggc cgcagcgctg
    28621 gtgccggact atggtgcgct gatgtcctgg cggatcgtgc ttttggccgt tgccctgtgc
    28681 ttgtggattg caccacagct aaaaggttat cagcctgcgg ttccgctact atccgtctca
    28741 ttcattctgc ttctggcagg ggaattaatc ggtcgcggcg tattctatgg tttgcatatg
    28801 accgtgggga tggccgtcgc aagctaatca gtaacaaccg gggtttcggc cccggttctc
    28861 ttttatttct gcttcgttaa cgtgtcataa ctggtcatca aattacgata atcaggaata
    28921 tggttagaga acaacgtcgc cagtccttca atatcattac gccagtcacg gtgcagctcg
    28981 caagccacgc cgaaccatgt catcaattgc gcaccagctt gcgacatgcg atcccacgcc
    29041 gaatgccggg taatttcatt aaaggtccct gatgcatcag tcacgacaaa gacatcaaac
    29101 ccttcttcaa tggctgaaag cgccgggaat gccacgcaaa cttcggttac cacaccggca
    29161 ataattaact gttttttacc tgtcgctttg actgctttta caaaatcttc gttatcccag
    29221 gcgttaatat tgcccgggcg agcaatgtaa ggtgcatcgg ggaattgtgc tttcagttct
    29281 agaactaatg ggccgttagg accggtttca aaactggtgg taagaatagt tggtaagttg
    29341 aaatacttag ccaggtcacc cagcgccagc acgttatttt taaacttatc gggttcgata
    29401 tcccgtacaa gggaaagtaa accagcctgg tgatcaacaa gcaaaacggc agcatcattt
    29461 ttatcaagac gaacatacgg tttggtcatc ctcttctcct ttcgagagtc agtggtacgt
    29521 ctgaaatcgg tcagacgcga tgcattgctc tgaaagcata gacgggaaat atgagtttgc
    29581 tgtgaccatg aaatttttcg actgaacgaa gcgtatgaaa tttgtgttag atcaataaaa
    29641 acaatcagat gagattaatc atctcttcag cattagcgtt atgaatgagt aatataaatc
    29701 gataataccg ccgctgcgat atgcgcgtat tttcacccat tgacaatgtt tttggcggtg
    29761 gcatgatgcg catgaaattt gaacttcctc acggttttaa ttcatgtcca cgtatacccg
    29821 gcctgtcatg cttttgctgt ctggcctgct tttgttgact ctggcgatcg cggtgttaaa
    29881 tacactcgtg ccgctttggc tcgcccagga acacatgtcc acatggcagg taggcgttgt
    29941 cagctcatcc tattttaccg gcaaccttgt cggtacattg ctgacagggt atgtcattaa
    30001 acgcattggc tttaaccgca gctattatct gtcctccttc atttttgctg ctggctgtgc
    30061 cggccttggc ctgatgattg gtttctggag ctggttggcg tggcgttttg tcgcgggcgt
    30121 cggctgtgcc atgatttggg tggttgttga gagcgcgctg atgtgcagtg ggacgtcacg
    30181 taaccgtggg cgtttgcttg ctgcgtatat gatggtttat tacgtgggaa cgtttttagg
    30241 ccagttactg gtcagcaaag tctcaaccga gctgatgagc gtattgccgt gggttacagg
    30301 tttgacattg gcagggatct taccgctgtt gtttacgcgt gtcctgaatc agcaggctga
    30361 aaaccatgat tcgacgtcaa ttacggcaat gctaaaactc cgtcaggcgc ggcttggcgt
    30421 gaatggctgc attatctcag gaatcgttct gggatctcta tatggcctga tgcctctgta
    30481 cctcaatcac aaaggggtga gcaatgccag tattggtttc tggatggcgg tactggtcag
    30541 tgcgggtatc ctcggacaat ggccgattgg acgtctggcg gataagtttg gtcgactgct
    30601 ggttttgcgt gttcaggtct ttgtcgtcat tctcggcagt attgcgatgc ttagccaggc
    30661 ggcgatggcc ccagcgttat tcatcctcgg tgccgctggc tttacgctat atccggtggc
    30721 gatggcctgg gcttgtgaga aagttgaaca tcatcaactg gtggcgatga accaagcctt
    30781 actgttgagc tataccgtgg gaagtctgct tggcccgtca tttagcgcta tgctaatgca
    30841 gaatttctcc gataatttat tgtttatcat gatcgccagc gtatcgttta tctatttgct
    30901 gatgctgctt cgcaacgccg gtcatacgcc gaaacccgtt gctcacgtgt aaatgaattc
    30961 aagcaaagtg tgaacttact gtttcacact ctgctttttt gtttcttcta tctgacttgc
    31021 ttttttccaa attttattcg tttaaaaata aaatgtgcag caggttataa ttttgcattt
    31081 cgctatttcc gcacttctta tttgccgcgc ataatccctc gttttacaga tgccccttta
    31141 attttggcga aggatttgtc tatggctgg
//
LOCUS       DAJTQQ010000040        30226 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000040 DAJTQQ010000000
VERSION     DAJTQQ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30226
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.040"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..746)
                     /gene="wcaD"
                     /locus_tag="OO309_002031"
     CDS             complement(<1..746)
                     /gene="wcaD"
                     /locus_tag="OO309_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR5847180.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALIS"
     gene            complement(721..1938)
                     /gene="wcaC"
                     /locus_tag="OO309_002032"
     CDS             complement(721..1938)
                     /gene="wcaC"
                     /locus_tag="OO309_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR5847181.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(1935..2423)
                     /gene="wcaB"
                     /locus_tag="OO309_002033"
     CDS             complement(1935..2423)
                     /gene="wcaB"
                     /locus_tag="OO309_002033"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR5847182.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2426..3265)
                     /gene="wcaA"
                     /locus_tag="OO309_002034"
                     /pseudo
     CDS             complement(2426..3265)
                     /gene="wcaA"
                     /locus_tag="OO309_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3358..5520)
                     /gene="wzc"
                     /locus_tag="OO309_002035"
     CDS             complement(3358..5520)
                     /gene="wzc"
                     /locus_tag="OO309_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR5847183.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNCIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5523..5966)
                     /gene="wzb"
                     /locus_tag="OO309_002036"
     CDS             complement(5523..5966)
                     /gene="wzb"
                     /locus_tag="OO309_002036"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR5847184.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(5972..7111)
                     /gene="wza"
                     /locus_tag="OO309_002037"
     CDS             complement(5972..7111)
                     /gene="wza"
                     /locus_tag="OO309_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR5847185.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7420..7569
                     /locus_tag="OO309_002038"
     CDS             7420..7569
                     /locus_tag="OO309_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5847186.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7770..9353
                     /locus_tag="OO309_002039"
     CDS             7770..9353
                     /locus_tag="OO309_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5847187.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9805..11658)
                     /gene="asmA"
                     /locus_tag="OO309_002040"
     CDS             complement(9805..11658)
                     /gene="asmA"
                     /locus_tag="OO309_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR5847188.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNSQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11680..12261)
                     /gene="dcd"
                     /locus_tag="OO309_002041"
     CDS             complement(11680..12261)
                     /gene="dcd"
                     /locus_tag="OO309_002041"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR5847189.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12353..12994)
                     /gene="udk"
                     /locus_tag="OO309_002042"
     CDS             complement(12353..12994)
                     /gene="udk"
                     /locus_tag="OO309_002042"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR5847190.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13312..13554
                     /locus_tag="OO309_002043"
                     /pseudo
     CDS             13312..13554
                     /locus_tag="OO309_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            13553..14494
                     /gene="mdtA"
                     /locus_tag="OO309_002044"
                     /pseudo
     CDS             13553..14494
                     /gene="mdtA"
                     /locus_tag="OO309_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            14494..17616
                     /gene="mdtB"
                     /locus_tag="OO309_002045"
                     /pseudo
     CDS             14494..17616
                     /gene="mdtB"
                     /locus_tag="OO309_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17617..20682
                     /gene="mdtC"
                     /locus_tag="OO309_002046"
     CDS             17617..20682
                     /gene="mdtC"
                     /locus_tag="OO309_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR5847191.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20683..22098
                     /locus_tag="OO309_002047"
     CDS             20683..22098
                     /locus_tag="OO309_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5847192.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            22095..23498
                     /gene="baeS"
                     /locus_tag="OO309_002048"
     CDS             22095..23498
                     /gene="baeS"
                     /locus_tag="OO309_002048"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR5847193.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            23495..24217
                     /gene="baeR"
                     /locus_tag="OO309_002049"
     CDS             23495..24217
                     /gene="baeR"
                     /locus_tag="OO309_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR5847194.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            24408..24740
                     /locus_tag="OO309_002050"
     CDS             24408..24740
                     /locus_tag="OO309_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR5847195.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24887..26248
                     /gene="yegQ"
                     /locus_tag="OO309_002051"
     CDS             24887..26248
                     /gene="yegQ"
                     /locus_tag="OO309_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR5847196.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26590..26907)
                     /locus_tag="OO309_002052"
     CDS             complement(26590..26907)
                     /locus_tag="OO309_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5847197.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            27313..28212
                     /gene="yegS"
                     /locus_tag="OO309_002053"
     CDS             27313..28212
                     /gene="yegS"
                     /locus_tag="OO309_002053"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR5847198.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(28294..29067)
                     /locus_tag="OO309_002054"
     CDS             complement(28294..29067)
                     /locus_tag="OO309_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5847199.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(29173..30213)
                     /gene="gatD"
                     /locus_tag="OO309_002055"
     CDS             complement(29173..30213)
                     /gene="gatD"
                     /locus_tag="OO309_002055"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5847200.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
BASE COUNT         6983 a         8029 c         7815 g         7399 t
ORIGIN      
        1 gaaatcaatg ctaacggtaa ttttttctta atcgcctctt tattcagata ttgaaatgcc
       61 cactccagca aataaaacag gataaaggtc ataacccctg aaaacgatcc ggataatatt
      121 atccctgcga gaatcagggc atctgtttta ggcgttttga taccaaactg tttgatgctg
      181 agccaaattg agattaatgc cagagcgaaa aatgccggtt cgaaataaag tgctgtcgtg
      241 cgcttgccgc cgaatttaat gaaattcagc acatagctgt tgctgtaaat cagatatttc
      301 gaaatcgact ccataatact gctgccaccg gtgaggataa tttgcgccat ctccaccgcc
      361 gccagcgcca ccaccagtcc gaccaccaga taaaagaaac gtaatatctt acgatggttg
      421 tgtggcgaaa ttgttttaaa acgaatactc cacaccatgc caataatgat cacaatatag
      481 acaaacagca tagttgaagt gacgtattta ccggcatcca acgactgacc aaacaggtag
      541 ttaaacgccg tgagtcccgc gccaatccct aaggcaatca tcaatttttt aatgctgatg
      601 cgttctaaaa acagcagtaa caagacaggt aaaaaagtga cgatggtgat ggggaaactt
      661 tcgccaagct gggcgatttt gacgttaacc agcaggtaga tcagtggcag cagcaggtag
      721 ctacagattc tgatagaagt tgacatactc ctccagcatc tgttgtccac tgtaggcggc
      781 gcgactgcgt tggctgaact cagccagcgt ggtaccaaat atcgcctgcg cgatttccgg
      841 tttgcttaac tgtaccagtt gcagcacctc ttcttcactg acggttttac caccggattt
      901 ttgcagcact tcccgcgccg catcgctatg ggtggcaatc accggcacgc caatcgatag
      961 cgcctcacac aaaatcagcg ggtagttatc gacacgagaa ctgaacacca gcgcatccat
     1021 ctgattgagc gcgctcatca acttgcgctt gtcagtttca aagccgtgat taaccacgtt
     1081 gccagcggtg aacggcgaga acttaccaaa ggtatgcagt tcgattttgt cgcccagcgc
     1141 catcatctca cgcaccagtt gctggttagt tttgccgtcg taacgcaggt catgcgccac
     1201 cactgcgatt ttcggcttgc cctgagtttc gcgcaccgga ggcagatctg ccagaatcgc
     1261 ttcggttgcc atatcaatgc cattattgat aatccggcaa cgccctggcc cgtacagact
     1321 attgaaagcg tcagccacat gctggctggg ggaaataaac tgacagccca gcgccagcat
     1381 ctcgcggaat aactggcgtt tgcccgccac cagctggtgc gcgcgatcaa tcttcaccgg
     1441 cggatagtta tttaaggtcg ggcatttctg gcagcccgtt ttccagcctt cgcaaccgtc
     1501 ggtaaaggcg cagcgtccgg ttacactcca gtggtcgtgc agcgtccaga ccagggtgac
     1561 atccggttta tggtttttca ctttttcgca aaagcgcacc acgctgttaa gattcaacca
     1621 gtagctgtgc agcacatgaa aatgcaggac caccggaccc ggtgtgcgag taatggtgcg
     1681 atataactca ttgaaattgc cgaacagatc gcgattaaac agacgaaaca acgcaatgtt
     1741 cgccatcgcg gtcatccgcg gcgtatgttt gatgacctgc ggatagttct gatggctgac
     1801 gctctctttg ccgcctttgc cgtagccgta gacaaaatgt gacgccagcc cctgttgcag
     1861 cgcacgctgg tggagatcta acgccacacc tgctgccccg ccttccgcca gtcgcacatt
     1921 aaattgcaga atattcattt aatcaccttc actcgcgctt tttctcccac caccagcgcg
     1981 ttgtccggga cagagtcgag caccacgctg cccgcaccca cggtgacgtt gttaccaagc
     2041 gtgatatcac caagaataat gacgttggca ccgagttcga cgccgttgcc aatgtgtgga
     2101 catgccatgt tatcggcacc ccgattgccg atagtgacgc cgtggcgaat ggtgaaatca
     2161 tcccccgcca ccacgttttt attgatcact acggcgtaac cgtgatggat agtaaagcgg
     2221 cggccaatgg tcgcggcggc ctggatttca taaccgaaaa agcattcggt gataatgcga
     2281 tacagcacca gcagcggggc cgcccacaga ttgttgagga cgttcttttt gcgccacacc
     2341 gaacaaaaat gagcgacgcg ataggcaagt accatgcagc acgggcgtaa actccagctg
     2401 ttggcgcgca gatcttccag catctttagc gcccccgaat accatcagcc agacgtttgc
     2461 cgttacgcac cgacagcagc gtcaacaagg tgcgccaggt catacgttta ttgcggatct
     2521 ggtagagggt aaacagctga tattttttac tggcgcggtc gaatttgtct ttgtgcttgc
     2581 ggtaaaagtg gaagtatccg gagaattttt tcggtgacga ggtgatctgc atttcgccgt
     2641 gattgatatg caggatctgc gtcgcctctt ccactttcca cggttcgccg tactccacca
     2701 ccatccgcag gaatatgtcg taatcctgtg ctgctttcag ttcggtatcg aacaaacact
     2761 ctttgaaacg ccatgcccag gtaaagacct gattgccaat gatattacgt ttatagaaca
     2821 ggcggcgtga gtacggcgat ttgggataca gtggtaagct cgccggttgg gaataaactt
     2881 ctccctagca aacgtagtcg ttagcgtaca aaaaggcgtg tgtgaccagt tgctgtttat
     2941 gggcgaggaa gacgctcagg cggttgggcg tccattcatc atcatcatcg atcccggtga
     3001 tgtattcccc ttgcgccagc ataatcgcct ggttacgcac cgcgcacgcc ccgctgttaa
     3061 tgtcgttgtg gatgtaagtg atacgcggat cgttgagggc agtgacgtac tgttgcagtt
     3121 gttcccagga ggtggagcaa tcatccacga tgatcatctc ccagttgctg tagtcctggc
     3181 gcaggaccga ttttatcgcc cgaatcgcca gttgttggcg gttccaggtt ggcatataga
     3241 ttgagatcag cggattgtct ttcataggtt gttctcccgg cttatgagct ggtttgcttg
     3301 ccggatgcgg cgtgagcgcc ttatccggcc tacggggcgg tgcgaatgca ggcgtcgtta
     3361 tttcgcatcc gacttatatt cgtattcgta atagccataa tcctgatacg cgctggcgcg
     3421 gcggaagatg gagttcagaa tcaccccttt caccggaata cagttttgct caaagcggct
     3481 cagactggtt tccacttctt tcaatgtgtt gaccgcataa cgcgccacca ttaacgtggt
     3541 tccgacatga cgaccaacaa ttgccgcatc ggtcactgcc agaatcggcg gcgtatcaat
     3601 caacaccagg tcatagtttt tactcgccca gttcaccagt tcggcaaagc gttcgctcat
     3661 taacagttca gaaggatttg gcggcacctg accgcgcggg atcaggtcaa atttggcaat
     3721 agaggtcggt ttagcagcgg tagtaatatc gccctgacca atcagaatct ccgacagccc
     3781 attgacatta ttggtgccca acagctcgtg ggtgtagcct ttgcgcatat cgcagtcgat
     3841 caacaacacg cgtttattgg tctggctgat caccgccgcc aggttggcgc agacaaaggt
     3901 tttaccgatt gacgggctaa ccccggtcat catcaacaca ttgttctgcg cctgcatcat
     3961 cgcgaagtgc agactggtac gcagactacg gatggcttca atcgccagat cggttggatt
     4021 ccccactgcc agtagctggc tctgtttata gcgtttaacc cctttgatgg ttttgacgct
     4081 atcgcgcgct ttctgccact ccgacagcgg gatgctggca tagacgctga ttccgtgttc
     4141 ttccagcact tgcgggcttt cgatgccgcg attaaacaac gagcgcagca gcacacccac
     4201 gatagagagc atcaggccaa ggataatcgc gccgaggata atcagccctt tcttcggttt
     4261 tagcacgcca ggctgggtga ttgccgggtc aataatgcgc acatcgccga cggtgctggc
     4321 ttcggtgatt ttcagctcct gctctttatt cagcagttgc atatagacct gctgaccaga
     4381 ctcgacatcg cgggtcagac ggacaatttc ctgctggttt ttcggcatcg ccgttacgcg
     4441 accgttaagt ttggctttct cttcttccag cgcctgacgt ttctccagca gcgtgcggta
     4501 cgccgggtga actttggtgt acagcttgga gatttccgcc tctttaaagg tcagttcgtt
     4561 caactgcgcg tcgatgttca ccatcgaatc gagcaccgct ttcgcttcaa gcggcagatc
     4621 aacagaatct ttatcctgac ggaaggcatt cagtttgttt tcggcaacat cgaggcggct
     4681 acgtacttcc ggtaactgtt gcgcgaggaa agcgaggctt ttcgacgctt ccgccgattt
     4741 gcgctcaata ttttgttcct gatagttacg agcgatgctg ttaagaatgt cgcggatctg
     4801 ttcgcgatct tcaccggtat aagtcaggct cagtacgcct gcgtctttgc cgttctccgt
     4861 taccgtcagg ctgttttgca gttggttgat catccccaac gtggagtatt tggtgacggt
     4921 aaactcactg cccgggcggg cgtgaatggc ttcaaccatc agcgtgacgc cttctttttt
     4981 cagcatctgg cccgcttgcc cacgggcgct aaagccgcca tcactgctca gggtgtagtt
     5041 tttgtcatcc agcacattaa gcgtaaacac ctgatccgcc atctctttcg ggcggttaaa
     5101 ggtagtcact ttcaccgtct cgttctgacg ccccatcagg cgatcccagc ccgccccgaa
     5161 aatcgggaac gtgtttttgc tcaccgcaat atcgaggtcg agatcgtcca ccgttttacc
     5221 aagcaccaga cgcgaacgaa tcaactggat ctcggcgtcc gatgcaggcg gtttgttggc
     5281 taatgccgag ccgatatcct gcaccagcga attgccgctg ttttgctcga tttgtaccag
     5341 tgcgtcggca ctataaatcg gcgtggcgaa gaaagtgtaa accacggcac agagggcaaa
     5401 tacgacggtg atgccaatca cccaccagcg cgcttcaatg acggtgccga ccaggcgacc
     5461 aatatcgatt tcatcactgc ccgttaccgg agcggcatgt tgttttactt tttctgtcat
     5521 tcttatacct gctctgcgtt caatgcctgc gcccactggc gggcagaccg ttcaagtaat
     5581 gtgtacaccg ctgcaaacgt ttcccggctt ttgtgatacg gatcggggat ttcacattcg
     5641 ttatcccagt gaccaaacag catcactttg ccgcgcatct ccggtgccat ctcgcataag
     5701 cgttcgatat ggcgcttatc catggtcaaa atcaggtcat agttgcgaca caggcggcgg
     5761 ctgatttgtc gtgcacagtg gccttccaga gacagttgat gttctgcggc gacgctgata
     5821 gcggtaggat cagcgccctt accgaccagc gcgccgagtc cagcggactc cactttcagt
     5881 tccgggtgat aacgttgcag taagcgttcc gccatcgggg aacggcaaat attgccgaca
     5941 cagacaacta agatgttgtt aaacatgacg attaccagtt atgaatgtcg ctggctgtat
     6001 ccgtcatgta acggacaccg ctaatagttg gcagcaactg attgatcaga cggttccagc
     6061 gggaaaccgg ggcggtggtg acatacacca cgtcataagg ttgcaggcgg aattctgtcg
     6121 ccatcaccag cgacgtcgca tcggacatat ccagctggta gatattggca atcttgccgt
     6181 tacgcccgcc ctcgcctttc agcggacgaa tgacaaagat gccgctggcg ttggaggtgg
     6241 tcatgtcgat accttcggca ttggccagag cttcagtcag ggtcatgccg ctaaagtcca
     6301 ttttcagggt gctctgtttc ttcacttcac ccatcacaaa tactttcaga tcatcattac
     6361 gcggcacgta gagaatatcg ccgggataaa gcagtcggtt ctgattgagg tcgccgtttt
     6421 gcatcagcgc ctgtaaagaa atgcgttctt cacgaccatt gtgtgttagc accacgttgc
     6481 gccagtcagc ggtgtcggtc aggccacctg cggcgttgat ggcgtcgaga atggtcagtg
     6541 gtacgttggt gatcgcctgt tgaccggatt tattcacctg acctgagata taggcctttt
     6601 gtgagcggaa ggcggcgata ttaacgtcca cctgcgggtc agcgatgtac gtcgctaagc
     6661 gcccggtaat atcactgcgg atttcagcga gcgttttccc gactacgtga accttgccga
     6721 tatacgggta aaacatagtg ccgtcaggct gtacccagtt gccggtgtcg ctggagctgc
     6781 ggtactgacc agctggcgtg gtgagttccg ggtgatccca gacggtgaca ttaagaacgt
     6841 cccccggccc gacgcgatac tggtaattcg cgatctcgct ttccagcgtc atattggggc
     6901 gcgctacatt cgggcgtggg cgtaattggt caatcaggcg cggggtcagc ggataaacat
     6961 tcaccatttt gtcgagatcg aaatcagcgt cctgctgttt gatgacgtct ttgcccatcg
     7021 tcgacatatt gctgcccgga agtactgtgc aaccgcttat caaggttact gacaccaata
     7081 atggcatcaa tttcattttg gatttcatca ttgtttattt atcactttgg cagagtaatt
     7141 atcctgtgca ctattaatag caatgtcgcc atgcacattt accttgcagt taattgaata
     7201 aaaatttaac tggcatcagt cctaaaaaaa ttgatttcat ccgcaggcta ttgacagaat
     7261 aattcagact ggtctttcag gcatccagac acgctaccgc ccctggcttt ttagctacca
     7321 atacactgat tgagcttaat ttttcaaacc ctctctgcat acaatcgttg atgagaaagg
     7381 gttattgcgg aaattaactt ccgaatataa ggtgacatta tggtaattga atattggctc
     7441 gccaataatg caggaggaag tgttacagct aacggaatag caggcaaggt aacaattcgg
     7501 caattggcta tttttaagaa ttatattaag tatcattcaa tatggttttt aggagtttct
     7561 ttaggttgac aatatttaat attgcgtctc cacatgcgat atttcttaaa taatgtttta
     7621 ttattaccgc ttttaattca gggataatgt gaggttatta ccctcaaata atatgagata
     7681 aatagtgctg caacattgca tttttgcccc gatttatcca tgatcgaatt gtgacatttg
     7741 tcatacaacg aataggtttt gtacttacta tggaatggat tgccgatccg tctatctggg
     7801 ccgggttaat cacgctgatt gtgatcgaac tggtcctcgg cattgataac ctggtcttta
     7861 ttgccatcct cgccgaaaaa ctaccgccga agcagcgcga ccgcgcacgg gttaccgggc
     7921 tgctgctggc gatgttaatg cgcctgttac tgctagcgtc aatctcctgg ctggtcaccc
     7981 tgactcaacc gctgttcagc ttccgctcgt ttacctttag cgcccgcgac ttaatcatgc
     8041 tgtttggtgg tttcttcctg ttgttcaaag ccacgatgga gctgaacgaa cggctggaag
     8101 ggaaagacag caataatccc acacaacgca aaggtgcgaa gttctggggc gtggtgacgc
     8161 aaattgtggt actggacgcc attttctcac tcgactcagt gattaccgcc gtcgggatgg
     8221 tcgaccattt actggtcatg atggccgccg tggttatcgc tatcagcctg atgttgatgg
     8281 ccagcaagcc gttaacgcaa tttgttaaca gtcacccgac catcgttatt ctctgcttaa
     8341 gcttcctgtt gatgattggc tttagcctgg tggcagaagg tttcggcttc gtcattccga
     8401 aaggctacct gtacgctgcc attggtttct cggtgatgat cgaggcactc aatcagttgg
     8461 ctatctttaa ccggcgacgt tttctttccg ctaatcagac gctacgccag cggactaccg
     8521 aagcggtaat gcgcctgctt agcgggcaaa aagaagatgc ggaactggac gccaaaaccg
     8581 cgtccatgct ggtggatcat ggaaaccagc agatctttaa tccgcaggaa cggcggatga
     8641 ttgagcgggt acttaatctt aaccagcgta ccgtaagcag cattatgacg tcgcgccacg
     8701 atattgagca tatcgatctc aacgcgccgg aagaagaaat ccgccagttg ctggagcgaa
     8761 atcagcatac gcggctggtg gttaccgacg gtgatgacgc agaagatttg ctcggtgttg
     8821 ttcacgttat cgacctgcta caacagtcac tgcgcggcga accgctcaac ctgcgggtgt
     8881 tgattcgcca gccgctggtg ttcccggaaa ccttgccgtt gttaccagcc ctggagcagt
     8941 tccgtaatgc ccgcacgcac tttgcttttg tggtggatga gtttggctcg gtggaaggga
     9001 ttgtgacatt aagtgacgtc actgaaacca ttgccggtaa cttaccgaac gaagtggaag
     9061 agatcgacgc ccgccatgat attcagaaga atgcggacgg ttcctggacg gcgaatggtc
     9121 atatgccgct ggaggatctg gtgcaatatg tgccgctgcc gctggatgaa aaacgtgaat
     9181 atcacaccat tgccgggctg ttgatggagt atttgcagcg tattccaaaa cctggcgaag
     9241 aagttcaggt gggggattat ttgcttaaaa cgttgcaggt ggaaagccat cgcgtgcaga
     9301 aggtgcagat tataccgctg cgtaaggatg gcgagatgga gtacgaggtg tgatggtatc
     9361 ggccatttta cccaatgctg cgtaacgcct tataacttca gcaatgaagc gttgtctgtg
     9421 gtaaacggac atcacagcgg gggtgtgggt ccggctgtaa atcgccgaat cgtttcctgt
     9481 aggctggggc agcccacatg gatgtgggct gagggcgcgt tttacaggga tgttacctcg
     9541 cgcccgtacc cgatagccga aaggaataag atgagggaac cgcgccagcg gcgatttacc
     9601 gccgggagcc cgggttgcca gggtggtggc gattgagcca ccctggcacg ttcaccggct
     9661 tgagcgttcc atagtagcga ggaacatcaa gtgaacggaa cagccttcga tcttacatat
     9721 tcagaatata atagtgccgc cgcgaacgcc tgatgcgacg ctttacgcgt cttatcaggc
     9781 ctacaggaca ggtcacctgg taacctacat cttctccagc aacttcttca catctttgcc
     9841 attgcgggaa tctttattcc gctcggccca gtcattcagg cgacgtttcg cttcgtcctg
     9901 tagatgtttg cgcagtaact gatccacttg caggctgtaa ttgagttgct gccagttgcc
     9961 ataaacccgc agcggtactg gcgtttcttt gaggaaatca atcagtttgc tttccccgtt
    10021 ccagccaccc acgacccgaa tatcaaactg ggtgtcgcag gtttgatctg ccagattcaa
    10081 catgccttcc cctgtcagcg ccagcacagg cgattgacct tgcatgtcgt ttaacgtcac
    10141 gacgccatcc ttcaacgtca aatcggtggt aaagcggtca agccgcgtca cgttatcgaa
    10201 gttttcagcg gccttcacat caccgccatt acgctctacc gcttgctgaa tcatctgctg
    10261 ggagttcatc ccttccatac gtgtgtcggt catctcaaca tgcgcctgtc cttgccagtt
    10321 gtggcggaat gcgtcggcat ctatgtcagc accggagaaa tcaccagcca gtgacatttt
    10381 tccggtcaac gaaatcggat agttaaacgc cttcagaatg gtaccaatct caacgttctc
    10441 cagccgtggc tggaagttta tccgcggatt tattgatgtc gcgtccagcg tgcccggcag
    10501 tgaaaccttt ccaccgttaa gcttgccctg cagttgagta atttccagca aacccgactt
    10561 gttggtcatt tgcgtggcaa catctgtaaa attcattccg cgccagcgca cgttactggc
    10621 ctgcaacaaa atatcagctg taaagccttg cagtccctga taggccggtt catcaatacg
    10681 cgaagaaatg accgggcgcg gcaacgttga ttggctctgc ccctgctgcg cggcaccgtt
    10741 ttcaccattc gcggtttcat taagcgggat gaggttgtca agattcagtt gcgggaattg
    10801 cagtctcagc tgccattccg gtttctctgt cagcgtgacc tgtgcttgtc cgctcagcgt
    10861 actgtcattg gcggtcaaac taatttggtt aaatgaaagg cgtttatgtg actcctgcca
    10921 ctgggactgg aaactcccct gcccctgaat accttgtttt ggtaaatcgg caccctgcaa
    10981 ctgccagtta atttgttcaa tagccgctgt taaatcatgc ggataatcag aagcatctac
    11041 cgtaccgtta agggatatcg tgagatcgcg ctgatcgcga ttaacccgcc cggagaactc
    11101 aaatgagcca cgatgttggg gatcttgttc catttgcagg cggatattgc ggattgtcac
    11161 ctgctcgtca tcttcatgct ggaacaccag aacgctgtcc gccaccttaa gactggatat
    11221 atcaaacgac catccgcgat cgtctgacag atccggcaag gtattgtcgc gcggtgcaac
    11281 cggagcgtct tcactgcgca ccgcttccgt ctgcggcgtc agttggatca ctgccccttt
    11341 tagcatcacc tgcttaacgc tcagttgatg actcagtagt ggtaaaagcg ccacgtccag
    11401 acgcatgttg tcggcgcgaa ccagtggctg gcttgcgccc tgggcggtga gagacattcg
    11461 cccggagagg atactaagct gcggccagac gtgccaacgc agtggcccgt cgagctgcaa
    11521 ttgataaccg ctacgtgcag caacttgctt gaccatatag tcgcggaaat cattcggatt
    11581 caccagcaac actaacgcag ataacccggc caccagcacg accaagagta tcatcagcgt
    11641 cgtcagaaat cgtctcatgg tatcctcaat gggcctgaat tagtctttat cgattcggct
    11701 ggctaccgcg ccctgctggt tgcgatattt cgcatcttca cggcggttgt aaggtcgcgc
    11761 cgccgggccg gaaagcggct caaagctcag cgcaccaatt aacatgcccg gacgcagcgc
    11821 cagcggcagc ttaccggagt tgtagaactc cagcacaatg caaccagacc agcccggatc
    11881 gatacggtgc gcggtgacgt gcaccatcag ccccagacgc gccaaagagg aacgcccgtc
    11941 cagccagccc accagatcgg ctggcagcgt caccgactcc agtgtcaccg ccagcgccag
    12001 ctctcctggg tgaagataga acgcctcgcc ctcgtcgaga acgatctcat cgctcatcac
    12061 gcggtcaagc gcggcgctca cttcatcttt gggaccgctc agatcgataa acgctgccgt
    12121 gtgaccacgg aaggtacgaa atttattgcc caggcgtaca tccaccgtcg cgccgttaat
    12181 acgctccact ggcggacgtg ggttgatcga caaacggcct tcatcaagcc aggcttcaat
    12241 atctcggtca cacagacgca tggcattttc tcctttcgca catcactccc ttaacgccaa
    12301 ttacgtcaag ggcataccaa cttatcactg aacggtacac aatttatcaa gcttattcaa
    12361 agaactgact tattttcgct ttcaatatat cgatcgcgat gcggtttttc ccgccgcgcg
    12421 gcacgataat gtccgcatat tgtttagaag gctcaatgaa ttgcaggaac atcgggcgca
    12481 cggttttttg atattgcgcc atcacggaat ccattgaacg cccacgctcg ttaacgtcac
    12541 gcttgatgcg gcgcatcagg cagatatcca gcggggtatc aacgaaaatg gagaagttaa
    12601 gttcgtcacg caaacgcgca tccgtcagca acaaaatgcc ttcgagaatg atgaccttct
    12661 tcggctcaac cgtcaccgtt tctttcatac gcgtatgttc aacatagctg taaaccggca
    12721 ggtcaattgc cgagccgcgt ttcaacgctt gtaaatgctc aagcaacagg ctgtgatcca
    12781 tcgcgctggg atggtcgtag ttggtcttaa cgcgttcttc catcgacaga tggctttgat
    12841 ctttgtaata gcagtcttcg ggaattacgc cgatgtgttc atcaccgact tgctcacgta
    12901 attcacgata aagggtactg gcaataagac tcttgccgga agccgatgcg ccagcgatac
    12961 cgataatgac gcactgatga gactgatcag tcatatattt agcgacctga ttaacctgga
    13021 tgttaggaag ggggcgacga agcgccaaac gcggcaatta tagggatttc atccgtctga
    13081 taccagtcga atagcgttgc cgcgcgctca gagttaattg ttgacaaaga attcccggtg
    13141 gcaaattacg ttgatcagtt ttatgtaagg taaaaaatgt tatacgcagt tgcgcaaatt
    13201 atccgccttt acgtcacttt atgagcaatt cgcatataaa atgtaaaact tttgtactag
    13261 cataaacaca gaaacgaata ctggcgacct ggccttgcgg ataaagcggt aataagcaaa
    13321 caatcacagc atgtattaat tgccctgccc cacccgctgc ttcacctggt cagtttaggt
    13381 ttagtctcgt ttatctttac ccttgtctcg cttgagcttt cgcagtttgg cacccaactc
    13441 gccccactgt ggttcccgac gtccatcatg atggtggcgt tttatcgcca tgccgggcgc
    13501 atgtggccgg gaattgcgct gagctgttcg ctgggaaata tcgccgcatc cattccagga
    13561 aggccagcag gtcaaagcag gcgatttact ggcagaaatt gaccccagcc agttcaaagt
    13621 tgcattagca caaacccagg gccaactggc aaaagataaa gccacgcttg ctaacgcccg
    13681 ccgtgatctg gcgcgttatc agcaactggc aaaaaccaat ctcgtctccc gtcaggagct
    13741 ggatgcccaa caggcgctgg tcagtgaaac cgaaggcacc attaaggctg atgaagcaag
    13801 cgtcgccagc gcgcagttgc aactcgactg gagccgcatc accgcaccag tcgatggtcg
    13861 cgttggtctc aagcaagttg atgttggtaa ccaaatctcc agtggtgata ccaccggaat
    13921 tgtggtgatc acccagacgc atcctatcga tttagtcttt accctgccgg aaagcgatat
    13981 cgctactgta gtgcaggcgc aaaaagccgg aaaaccgctg atggtagaag cctgggatcg
    14041 caccaattcg aagaaattaa gtgaaggcac gctgttaagc ctcgataacc aaatcgatgc
    14101 cacaaccggt acgattaaag tgaaagcacg ctttaataat caggatgatg cgctgtttcc
    14161 caatcagttt gttaacgcgc gcatgttagt cgacaccgaa caaaacgccg tagtgatccc
    14221 aacagccgcc ctgcaaatgg gcaatgaagg ccattttgtc tgggtgctga atagcgaaaa
    14281 caaggtcagc aaacatctgg tgacgccggg cattcaggac agtcagaaag tggtgatccg
    14341 tgcaggtatt tctgcgggcg atcgcgtggt gacagacggc attgatcgcc tgaccgaagg
    14401 ggcgaaagtg gaagtggtgg aagcccagag cgccaccact ccggaagaga aagccaccag
    14461 ccgcgaatac gcgaaaaaag gagcacgctc ctgatgcagg tgttaccccc gagcagcaca
    14521 ggcggcccgt cgcgcctgtt tattatgcgt cctgtcgcca ccacgctgct gatggtggcg
    14581 atcttactcg ccgggattat cggttatcgc gccctgcccg tttcggcgct gccggaagtg
    14641 gactatccga ccattcaggt ggtcacgctc tacccaggtg ccagcccgga tgtcatgacc
    14701 tctgccgtta ccgcgccgct cgaacgccag ttcgggcaga tgtctggcct gaaacagatg
    14761 tcgtcgcaaa gttccggcgg tgcgtcagtt atcactttgc agttccagct aacattaccg
    14821 ctcaatgtcg ccgagcagga agtgcaggcc gcgattaacg ctgcgaccaa cttgttgccg
    14881 agcgatctgc ctaacccgcc ggtttacagc aaagtgaacc cggcagatcc gccgatcatg
    14941 acgctcgccg tcacctcaac cgccatgccg atgacgcaag tggaagatat ggtggaaacc
    15001 cgcgtcgcgc agaaaatctc gcagatttcc ggcgtcggcc tggtgacgct ttccggcggt
    15061 cagcgtccgg ctgttcgcgt caaacttaac gctcaggcga ttgccgccct cggcctgacc
    15121 agcgaaaccg tgcgcaccgc cattaccggc gctaacgtta actcggcaaa aggtagcctc
    15181 gacggccctt cccgtgcggt cacgctttcc gcgaacgacc agatgcaatc cgccgaagag
    15241 tatcgccagc taatcatcgc ctaccagaac ggcgcgccaa ttcgtctggg cgatgtcgca
    15301 actgtagagc aaggtgcaga aaacagctgg ctcggcgcgt gggcgaacaa agaacaggcc
    15361 attgtgatga atgttcagcg ccagcccggt gctaacatta tctccaccgc cgacagcatt
    15421 cggcagatgc tgccacagct cactgagagt ctgccgaaat cggtgaaggt gacagtgctt
    15481 tccgatcgca ccaccaatat ccgcgcatcc gtcgatgata ctcagtttga attgatgatg
    15541 gctatcgcgc tggtagtcat gattatctac ctgtttttgc gcaatattcc ggcgaccatc
    15601 attcccggtg ttgctgtacc gctgtcgtta atcggcactt tcgcggttat ggtgtttctc
    15661 gatttttcaa tcaataacct gacactgatg gctttaacta tcgccaccgg attcgtggtc
    15721 gatgacgcca tcgtggtgat cgaaaacatt tcccgctata tcgaaaaagg cgaaaaaccg
    15781 ttggcggcgg cgctcaaggg cgcaggtgaa atcggcttta ccattatctc gctgaccttc
    15841 tcactgattg cggtgttgat cccactgctg tttatgggcg atatcgtcgg gcgactgttc
    15901 cgcgaatttg ctattaccct ggcggtagcg attttgatct cagcggtggt gtcgctgacc
    15961 ctgacaccga tgatgtgcgc gcggatgctc agccaggagt cgttgcgtaa acagaacagc
    16021 ttctcccgtg cctcggaaaa aatgttcgac aggataatcg ccgcctatgg tcgtggactg
    16081 gcgaaagtgc tgaatcatcc gtggctgacc ttaagcgtgg cactcagcac gctgctgctt
    16141 agcgtgctgc tgtgggtgtt cattccgaaa ggtttcttcc cggtacagga caacggcatt
    16201 attcagggca ctttgcaggc accgcaatcc agctcctttg ccaatatggc ccagcgacaa
    16261 cgccaggtcg cggacgtgat tttgcaggat ccggcagtgc aaagcctgac ctcatttgtt
    16321 ggcgttgatg gcactaaccc gtcgctgaat agtgcacgtt tacaaatcaa cctcaaaccg
    16381 ttggatgaac gtgatgatcg ggtgcaaaaa gtcatcgccc gtctgcaaac ggcggtagat
    16441 aaagtgccgg gcgtcgatct cttcctgcaa ccaacgcagg acctgaccat cgatactcag
    16501 gtcagccgca cccagtacca gtttaccttg caggccacgt cactggatgc gctcagtacc
    16561 tgggtgccac agttgatgga aaaactccag caactgccac agctttctga tgtctccagc
    16621 gactggcagg acaaagggct ggtggcgtat gtcaatgttg atcgcgacag cgccagccgt
    16681 ctggggatca gcatggcgga cgtcgataac gccctgtaca acgcgtttgg tcagcgactg
    16741 atttccacta tttatactca ggctaaccag tatcgcgtgg tgctggagca caataccgaa
    16801 aataccccag gcctcgcggc gctggatacc attcgcctga ccagcagcga cggtggcgtg
    16861 gtgccgttaa gctcaattgc caaaattgag cagcgttttg cgccgctttc catcaaccat
    16921 ctggatcagt tcccggtaac aaccatctcc tttaacgtgc cggataacta ttcgctgggc
    16981 gatgcggtgc aggcgattat ggacaccgaa aagacgctga atctgccggt ggatatcacc
    17041 acgcaattct agggcagcac cctcgccttc cagtcggcgc tgggcagcac tgtctggctg
    17101 attgtcgcgg cggtggtggc gatgtatatc gtgctcggca ttctgtacga gagctttatt
    17161 cacccgatca ccattctctc gacgctaccc accgcagggg tcggcgcgct gctggcatta
    17221 atgattgccg gtagcgaact ggatgtgatt gcgattatcg gcattatttt gctgatcggt
    17281 attgtgaaga agaacgccat catgatgatc gacttcgcgc tggctgctga gcgcgagcaa
    17341 ggcatgtcgc cgcgcgatgc aatctaccag gcttgtctgt tgcgttttcg tccgatcctg
    17401 atgaccaccc tggcggctct gcttggcgcg ctgccgctga tgttgagtac cggggtcggc
    17461 gcggaactgc gtcgtccgtt aggtatcggc atggtcggcg gtctgattgt cagccaggtg
    17521 ctgacgctgt tcaccacgcc ggtgatttat ctgctgttcg accgcctggc attgtggacc
    17581 aaaagtcgct ttgctcgcca tgaagaggag gcgtaagtga agttttttgc cctcttcatt
    17641 taccgcccgg tggcgacgat tttactgtcg gtcgccatta ccctgtgcgg catactgggt
    17701 ttccgtatgc tgccggtcgc cccgctgccg caggtcgatt ttccggtgat tatggtcagc
    17761 gcctcgctgc ccggtgcgtc gccggagaca atggcgtctt ccgttgccac gccgctggag
    17821 cgctcacttg ggcgcattgc cggggttagt gaaatgacct ccagcagttc gctcggcagc
    17881 acgcgcatta ttttgcagtt tgattttgac cgggatatca acggcgcagc gcgtgatgtg
    17941 caggcggcga tcaacgctgc acaaagtttg ctgcccagcg ggatgcccag ccgcccgacc
    18001 tatcgcaaag cgaacccgtc ggatgcgcca attatgatcc tcacgctgac atccgatact
    18061 tattcgcagg gtgaactgta cgatttcgcc tcgacgcagc tggctccgac gatttcgcaa
    18121 atcgacggtg tcggtgatgt cgatgtcggt ggcagctcgc tgcccgccgt gcgcgtcggg
    18181 ctgaccccgc aggcgctgtt taatcagggc gtgtcgctgg acgacgtacg cactgccatc
    18241 agcaatgcca acgtgcgtaa accgcagggg gcgctggaag atggcactca ccgctggcag
    18301 atccagacca acgatgaact gaaaaccgcc gctgaatatc agccgctgat tattcactac
    18361 aacaacggcg gcgcggttcg tctgggcgat gtggcgacgg tgactgactc agtgcaggat
    18421 gtgcgcaacg ccgggatgac caacgccaaa ccggctattt tactgatgat ccgcaaactg
    18481 ccggaagcca atattatcca gacggtagac agcatccggg caaaattacc agagttgcag
    18541 gagaccattc cggcggcgat tgatctgcaa attgcccagg atcgctcccc caccattcgc
    18601 gcctcgctgg aagaagtcga gcaaacgctg attatctcgg tggcgctggt gattctggtg
    18661 gtctttttat tcctgcgctc gggtcgcgcc actattattc ccgccgttgc ggtgccggtt
    18721 tcgctgattg gtacgtttgc ggcgatgtac ctgtgcggat tcagtctcaa taatctttcg
    18781 ctgatggcgc tcaccattgc tactggtttc gtggtggatg acgccattgt ggtgctggaa
    18841 aacattgccc gtcatctgga agcggggatg aaaccactac aggccgcgct gcaaggtact
    18901 cgcgaagtcg gctttacggt gctgtcgatg agtctgtcac tggtggcggt gttcctgccg
    18961 ctgctgttga tgggcggatt gccgggccga ctgttacgcg agtttgccgt gacgctttct
    19021 gtcgccattg gtatttcgtt gctggtttcc ctgacgctaa cgccaatgat gtgcggctgg
    19081 atgctaaaag ccagcaagcc gcgtgagcaa aagcgactgc gtggttttgg ccgcatgttg
    19141 gtagccctgc aacaaggcta cggcaagtcg ctgaaatggg tgctcaatca tacccgtctg
    19201 gtgggcgtgg tgctgcttgg caccattgcg ctgaatatct cgatcccgaa aaccttcttc
    19261 ccggagcagg acaccggcgt actgatgggt gggatacagg cggatcagag tatttcgttt
    19321 caggcgatgc gcggcaaatt gcaggatttc atgaaaatta tccgtgacga tccggcagtg
    19381 gataatgtca ccggctttac cggcggttca cgagttaaca gtgggatgat gtttatcacc
    19441 ctcaagccac gcgacgaacg cagcgaaacg gcgcagcaaa ttatcgaccg tctgcgcgtg
    19501 aagctggcga aagaaccggg ggcgaatctg ttcctgatgg cggtacaaga tattcgcgtt
    19561 ggtgggcgtc agtcgaacgc cagctaccag tacacgttgt tatccgacga cctggcggca
    19621 ctgcgcgaat gggagccgaa aatccgcaaa aaactggcga cgttgccgga actggcggac
    19681 gtgaactccg atcagcagga taacggcgcg gagatgaatc tggtttacga ccgcgacacc
    19741 atggcacggc tgggaatcga cgtacaggcc gccaacagtc tgttaaataa cgccttcggt
    19801 cagcggcaaa tctcgaccat ttaccagccg atgaaccagt ataaagtggt gatggaagtg
    19861 gatccgcgct atacccagga catcagtgcg ctggaaaaaa tgttcgttat caataacgaa
    19921 ggcaaagcga tcccgctgtc gtatttcgct aaatggcaac cggcgaatgc cccactatcg
    19981 gtgaatcatc agggattatc ggcggcctcg accatctcgt tcaacctgcc gaccggaaaa
    20041 tcgctctcgg acgccagtgc ggcgatcgat cgcgcaatga cccagcttgg tgtgccttcg
    20101 acggtgcgcg gcagttttgc cggcacggcg caggttttcc aggagacgat gaactcgcag
    20161 gtgatcctga ttatcgccgc catcgccacg gtgtatatcg tgctgggtat cctttacgag
    20221 agttacgtac atccgctgac gattctctcc accctgccct cggcgggcgt tggagcgctg
    20281 ttggcgctgg agctgttcaa tgccccgttc agcctaatcg ccctgatagg gatcatgcta
    20341 ttaatcggca tcgtgaagaa aaacgccatt atgatggtcg attttgcgct tgaagcccaa
    20401 cggcacggta acctgacgcc gcaggaagct attttccagg cctgtctgct gcgttttcgc
    20461 ccgattatga tgaccaccct ggcggcgctg tttggcgcgc tgccgctggt attgtcgggc
    20521 ggtgacggct cggagctgcg gcaacccctg gagatcacca ttgtcggcgg tctggtgatg
    20581 agccagctcc tgacgctgta taccacgccg gtggtgtatc tctttttcga ccgtctgcgg
    20641 ctgcgttttt cgcgtaaacc taaacaaacg gtaaccgagt aaatgacaga tcttcccgac
    20701 agcacccgct ggcaattatg gattgtggct ttcggcttct ttatgcagtc gctggacacc
    20761 accatcgtaa acaccgccct tccctcaatg gcgcaaagcc tcggggaaag tccgttgcat
    20821 atgcacatgg tcattgtctc ttatgtgctg accgtggcgg tgatgctgcc cgccagcggc
    20881 tggctggcgg acaaagtcgg cgtgcgcaat attttcttta ccgccatcgt gctgtttact
    20941 ctcggttcac tgttttgcgc gctttccggc acgctgaacg aactgttgct ggcacgcgcg
    21001 ttacagggcg ttggcggtgc gatgatggtg ccggtcggca ggttgacggt gatgaaaatc
    21061 gtaccgcgcg agcaatatat ggcggcgatg acctttgtca cgttacccgg tcaggtcggt
    21121 ccgctgctcg gtccggcgct cggcggtctg ctggtagaat acgcgtcgtg gcactggatc
    21181 tttttgatca acattccggt ggggattatc ggtgcgatcg ccacattgat gttaatgccg
    21241 aactacacca tgcagacgcg gcgctttgat ctttccggct ttttattgct ggcggttggc
    21301 atggcggtat taaccctggc gctggacggc agtaaaggta caggtttatc gccgctggcg
    21361 attgctggcc tggtcgcagt tggcgtggtg gcactggtgc tttatctgct gcacgccaga
    21421 aataacaacc gcgccctgtt cagtctgaaa ctgttccgca ctcgtacctt ttcgctgggc
    21481 ctggcgggga gctttgccgg acgtattggc agtggcatgt tgccctttat gacaccggtt
    21541 ttcctgcaaa ttggcctcgg tttctcgccg tttcatgccg gactgatgat gatcccgatg
    21601 gtgcttggca gcatgggaat gaagcgaatt gtggtacagg tggtgaatcg ctttggttat
    21661 cgtcgggtac tggtagcgac cacgctgggt ctgtcgctgg tcaccctgtt gtttatgact
    21721 accgctctgt tgggctggta ctacgttttg ccgttcgtcc tgtttttaca agggatggtc
    21781 aactcgaccc gtttctcctc catgaacacc ctgacgctga aagatctccc ggacaatctg
    21841 gcgagcagcg gaaacagcct gctgtcgatg attatgcaat tgtcgatgag tatcggcgtc
    21901 accatcgccg ggctgttgct ggggcttttt ggttcgcagc atatcagcgt cgacagcggc
    21961 accacacaaa ccgtctttat gtacacctgg cttagcatgg cgtttatcat cgcccttccg
    22021 gcgttcatct ttgccagagt gccgaacgat acgcatcaaa atgtagctat ttcgcggcga
    22081 aaaaggagcg cgcaatgaag ttctggcgac ccggtattac cggcaaactg tttctggcga
    22141 ttttcgccac ctgcattgtc ttgctgatca gtatgcactg ggcggtgcgt atcagttttg
    22201 agcgtggctt tattgattac atcaaacacg gcaatgaaca gcgattacag ctgttaagtg
    22261 atgcgcttgg cgagcagtat gcgcagcatg gtaactggcg cttcctgcgc aacaacgatc
    22321 gctttgtctt tcagatcctg cgttcatttg aacacgataa ttcggaagat aaacccggcc
    22381 cgggtatgcc gccgcacggc tggcgtaccc agttctgggt ggtcgatcaa aacaacaaag
    22441 tactggttgg tccgcgagcg ccgattccac ctgacggtac acggcgaccc attctggtca
    22501 acggtgcgga agtgggcgcg gtgattgcct cccctgttga gcggctgacc cgcaatactg
    22561 atatcaattt cgataaacaa cagcggcaaa ccagctggct gatcgtcgcc ctggcaacgt
    22621 tactcgcggc gctcgccacc tttctgctgg cgcgcggttt gctggcaccg gtaaaacgac
    22681 ttgtcgatgg cacgcacaaa ctggcggcgg gcgatttcac tacccgcgta acgcccacca
    22741 gtgaagatga actgggcaaa ctggcgcaag acttcaacca gctcgccagc acgctggaga
    22801 aaaaccagca aatgcggcgc gattttatgg ccgatatttc tcacgaactg cgtacgccat
    22861 tagcggtgct gcgcggtgaa ctggaagcca ttcaggatgg cgtgcgcaaa ttcacgccgg
    22921 agacggtggc gtctttacag gcggaggtcg gtacactgac caaactggtt gacgatcttc
    22981 atcagttgtc gatgtctgat gaaggcgctc tcgcctatca aaaagcaccg gtagatttga
    23041 tcccactgct ggaagtggcg ggcggcgcat ttcgcgaacg attcgccagc cgtggcctga
    23101 aactgcaatt ttccctgcca gacagtatca ccgtatttgg cgatcgcgac cgtttaatgc
    23161 agttattcaa taacttactg gaaaacagcc tgcgctacac tgacagcggc ggcagcctga
    23221 aaatctctgc cgagcagcac gacaaaacgg tgcgcctgac ctttgccgac agtgcgccag
    23281 gtgtcagtga cgatcagcta caaaaattgt ttgaacgttt ttatcgcacc gaaggttccc
    23341 gcaaccgcgc cagcggcggt tccgggctgg ggctggcgat ttgcctgaac attgttgaag
    23401 cccataatgg ccgcattatt gccgcccatt cgccttttgg cggggtaagc attacagtag
    23461 agttaccgct ggaacgggat ttacagagag aagtatgacc gagttaccaa tcgacgaaaa
    23521 cacaccgcgt attttgatcg tggaagatga accgaagctg gggcagttgc tcattgatta
    23581 tctgcgtgct gcgagctatg cgccgacgct tatcagccac ggcgatcagg tactggcgta
    23641 tgtgcgccag acaccaccgg atctgatcct gttggatctg atgctccctg gcaccgatgg
    23701 cctgacatta tgccgggaaa ttcgtcgttt ttccgatgtt ccgatcgtga tggtgacggc
    23761 aaaaatcgaa gagatcgacc gcctgctggg gctggagatt ggcgccgatg actacatctg
    23821 caagccgtac agcccacggg aagtggtagc gcgtgtcaaa accattttgc gccgttgtaa
    23881 accgcaacgc gaacttcagc aacaggatgc cgaaagcccg ttgattatcg acgaaggtcg
    23941 ttttcaggct tcatggcgcg gtaaaatgct tgacctgacg cctgcggaat ttcgtctgct
    24001 taaaacgctc tctcacgaac caggaaaagt gttctcccgc gagcaattgc tcaatcatct
    24061 ttatgacgac taccgcgtag taaccgaccg caccattgac agccacatta aaaatctgcg
    24121 ccgcaagctg gaatctctcg acgccgaaca gtcatttatc cgcgccgttt acggcgttgg
    24181 ttaccgctgg gaagccgacg cctgccgcat cgtttagttt tagcgacatt actttgttag
    24241 ccggagatgg cgttcagacc cgatccggca acattattcc acgcatggtc agcaaactga
    24301 cattatctct ccgccctggc aggcatcaga aaatcgctca tactttaatc agtaaacagc
    24361 acctttagat gctgttttcg ataagcaatt tcaatcaagg agtcattatg gctggttggt
    24421 ttgaactcag taagagcagt gataatcagt tccggtttgt gctaaaagcg ggcaatggtg
    24481 agactatcct caccagcgag ctgtatacct caaaagcctc tgcggaaaag ggcatcgcgt
    24541 cggtgcgtag caacagcccg caagaagagc gctatgagaa aaaaacggca agcaacggca
    24601 aattctattt caatctgaaa gccgctaatc atcaaattat cggctccagc cagatgtacg
    24661 ccaccgcgca atctcgtgaa accggaattg cctccgttaa agccaatggc acaagccaga
    24721 cggtgaaaga caatacgtaa ttacgatgcc gggcgtgggt tgttggcgtc cggcgcttta
    24781 ccagatcccc cctacagcgc tacaatgccc gcccttaaag tgggggcctc ccctaaccgc
    24841 ttcatcaggt gaagcggatc tgacctgtca tcagaacgag agaattatgt ttaaaccgga
    24901 actcctttcc ccggcgggaa cgctgaaaaa tatgcgttac gctttcgctt atggcgcaga
    24961 tgctgtttat gcgggccagc cgcgttactc cctacgtgtg cgcaacaacg aattcaacca
    25021 cgaaaatctt cagctcggca tcaatgaagc ccacgcgctg gggaaaaagt tttatgtcgt
    25081 ggtcaacatt gcaccgcaca acgccaagct gaaaaccttt atccgtgacc tgaaaccggt
    25141 ggtggaaatg gggccggatg cgctgattat gtccgatcca gggctgatta tgctggtgcg
    25201 tgagcacttc cctgaaatgc cgatccacct ctcggtgcag gctaacgccg tgaactgggc
    25261 gacggtgaaa ttctggcagc aaatgggact gacccgcgtg atcctctctc gcgagctgtc
    25321 gctggaagag attgaagaga tccataatca ggtgccggat atggagatcg agatcttcgt
    25381 tcacggcgca ctgtgcatgg cctactccgg tcgctgcctg ctctctggct atatcaacaa
    25441 gcgcgacccg aaccagggca cctgcaccaa cgcctgccgc tgggagtaca acgtccagga
    25501 agggaaagaa gatgacgttg gcaacatcgt acacaagtac gagccgattc cggtgcaaaa
    25561 tgttgagccg acgctgggta tcggcgcgcc aaccgacaaa gtgtttatga tcgaagaagc
    25621 ccagcgtccg ggcgagtata tgaccgcgtt tgaagatgag cacggcactt acatcatgaa
    25681 ctcgaaagat ctgcgcgcca tcgcccatgt agaacgcctg accaaaatgg gcgtgcattc
    25741 gctgaaaatc gaaggtcgta ccaaatcttt ctactattgc gcacgcaccg cacaggttta
    25801 ccgcaaagct atcgatgacg ccgctgcggg aaaaccgttc gataccagcc tgctggaaac
    25861 tctggaaggt ctggcgcatc gtggctatac cgaaggtttc ctgcgtcgtc atactcacga
    25921 cgattatcag aactacgaat acggttattc agtttctgac cgccagcagt ttgttggtga
    25981 gtttaccggt gagcgcaagg gggacctcgc agcggtagcg gtgaaaaata aattctccgt
    26041 tggcgacagt cttgagctga tgacgccgca aggcaacatt aactttaccc ttgagcacat
    26101 ggaaaacgcc aaaggcgaag ctatgccggt cgcaccaggc gatggttata ctgtgtggct
    26161 cccggtcccg caggatcttg agctaaatta cgcgctgcta atgcgtaatt tctccgggga
    26221 aaccacgcgt aacccccacg gtaagtgatt aatttcgatt atttttcccg gatggaaaat
    26281 tcttagaaac cgatcacata cagctgcatt tattaaggtt atcatccgtt tcgctgaaaa
    26341 acataaccca taaaatgcta gctgtaccag gaaccacctc cttagcctgt gtaatctccc
    26401 ttacacgggc ttattttttg tacataagct gtaaagaagg attgatccct gataaccgtc
    26461 ttccattgac cacatcgata gaatccccct tcatagcaca atgcctttca cgtaacggta
    26521 tcgtgctcgc ccaggttccg gctacgcaca gccagaacgc gcatgtttga cgcttacaaa
    26581 aaaatattct cactctccac atttgaatgt cagacgagcg acgccatgta atcctgcacc
    26641 ttctgtcttc aggtcaacta tctgcatttt tttgccctga gtaacacaga aatgggctgc
    26701 atcatttttt actatatttt ctgcaccaga tattctgccc ctggctaaag aagcttcggc
    26761 ttcggtgtag tattggttat cgagtttacg ctgaatatta cttttatatg caagaccaaa
    26821 tttaccgata cttgtttcat cattatgcac agcacaacca gatataagaa aaatactaat
    26881 taatgatata gcagctatct ttttcatctc accttccccc attaaatacc aacgacactc
    26941 tctagtgttt aaatataata atggcatgat tattataatt gaataggatt ataataaatg
    27001 ttctgtacaa catttcctac ataagtagga attacggaca ttgaggccct tcagggtaac
    27061 tccatggggg ctttaatata ttatattgaa gatgccactg tttagttgaa tattaggtat
    27121 atgctctttt ttgaaattta tcggtggcag ccgttagtat tcgctgtccc cattgcaagc
    27181 tcctggtggt aaccactgaa tcctccatac ttgaactgac tttttatcct ccgactttca
    27241 tcctgttctg actccacctt ttgttttctg ctctacacta tctacagacc aatcataaag
    27301 gcacatacga tcatggcaga atttcccgcc agcttactga ttcttaatgg taaaagtact
    27361 gacaatctac ccttgcgcga agcaattatg ctgttgcgtg aggaaggaat gacgatccat
    27421 gtgcgggtca cctgggagaa aggtgatgcc gcacgatttg tagaggaggc ccggaagttg
    27481 ggcgtcgcaa cggtgattgc cggtggtggc gatggcacca ttaatgaagt ttctacggcg
    27541 ttgattcagt gtgaggggga tgacataccc gcgctgggaa ttttgccatt aggaaccgcc
    27601 aatgattttg ccaccagtgt agggattcct gaggcactgg ataaggcgct gaaactggca
    27661 attgccggta atgccattgc gatagatatg gcgcaggtca acaaacaaac ctgttttatt
    27721 aatatggcga caggcggatt tgggacgcgt attaccacag aaacgccgga aaaattaaaa
    27781 gccgcgctgg gtggcgtctc ttacatcatt catggcttaa tgcgcatgga tactctgcaa
    27841 ccggaccgtt gtgaaatccg cggtgaaaac tttcactggc aaggtgacgc cctggtcatt
    27901 ggtattggta acgggcgtca ggccggtggc ggtcaacaat tgtgcccgaa cgcgttaatt
    27961 aacgatggct tgctgcaact gcgcattttt accggcgatg aaattcttcc ggctctcgta
    28021 tcaaccttaa aatctgacga agataacccg aatattatcg aaggcgcttc gtcgtggttt
    28081 gatatacaag ccccacacga aatcactttt aatcttgatg gcgaaccgtt gagtgggcaa
    28141 aacttccata ttgaaatact tccggcggcg ttgcgttgtc gattaccacc agattgtcca
    28201 ttattgcgtt aatcaaacta ttctctcttg aaccagggag aatccgtaat gccggatgcg
    28261 gtgtaaacac cttatccggc atacagaaca atactacacc gtaattaatt ttacccgtgt
    28321 cttctgcaat gccaatttat ccacctctga cacagcatca tcggtaacaa taatgtcaat
    28381 tttttcgatt ggcagtacct gattaaaacc gcgacgatta aatttcgatg aatcgagaac
    28441 ggcaatcact ttatttgcgg cggtgaccat cgccccgcta atcgaataac cttcattaaa
    28501 ggtcgtaata ccattaaccg catcaatacc atcagcaccg acaaacatta aatcggcatt
    28561 aatatcctgc aatgaacgct cggcaatgga accatgcatc gagcgcgttt tatggcggac
    28621 cgtgccacca cagacgacca gagtaatgtc tttattttcg gaaagggcaa acgctgccgg
    28681 gagactgttg gtaatcaccg tgatattttt agcggtcatt aatccttcgg cgataagcat
    28741 tgtggtactt ccgctgtcga gaataacggt catcccttca tggatcattt tgactgccgc
    28801 ctgcgctatt ctgttttttg gcgcgctggc gagctgaaat cgctctttaa acccgacttc
    28861 ctgggtctcg gtttcactat taccggacat tattttcgcc gcaccgccat gaaagcgcgt
    28921 aaccacgcct ttttgttcga gaaagcgcaa atcggcacgg attgtcgctt ccgaggcagc
    28981 aaatactccc gccagatcct gaacaagcac ggttccctgt tcattcacta attggatgat
    29041 cttattcctt cgctcgaatg aattcatcgt catttaccca tattcaattg tggctagtgt
    29101 aaacgaaggg ggaccattgg tgaatgatct gttttgcaca ataccgggcc agcatcacgc
    29161 tggcccgcgg tttcatggaa tgagcaacac tttgccctgc atggcattac gagcgatgtc
    29221 acgcaccgcc tgggtgaagc tttcaaagct tccacggtga gcgattaatg gctccaggct
    29281 taacttacgt tctgtcagca accggctcgc cgtttcccac tcctgccccg gccaagggct
    29341 ggaatagttc atccaactgc cgataaccgt cagctcttta cgcaatattt tgccaaacgt
    29401 tgccgatgtt aaatgcagat cctgatgcaa cgtgcccacc agcgccagtt gggcatgagg
    29461 accggcaatc tctaccgcca gttcgacagt ttgcggtacg ccagccgtct cgaggataag
    29521 ctgattaaag cgcagttcgc gtaaaacgct ctgcatttgc ggcgcgctca tttcgctact
    29581 gttaaatgtt tgcatcgcac cgaaagattt tgccagtgcc agtttttctg agctaatgtc
    29641 gatcgccgtc acactctttg ctcccagcgc gacagcgcac tgaatagcca gcaggccaat
    29701 ggttccggca ccaataataa taacgttttt attctcacaa ccttgcgcta aatgaaaagc
    29761 atgcaggcca acggtaatcg gctcaataaa agccccatcc tcaataggca tatccgtggg
    29821 tagagcaaag acattttttc gcttaacgac aatatattca gcaaatccac catcacgccg
    29881 cgagccaata aaatcatatt ttgcgcactg ggaataaaac cctttcaaac actctggaca
    29941 agtaaaacag ggtaataacg gcacacaggc aaccgcatcg ccagggtgta aatcatcaac
    30001 accggatccc accgcatcaa tatagccgct aaattcatgg cctaacgtta ttggataata
    30061 atgtgcacca tttttaaata tcctgggtaa atcggaacca cataagcccg agctggcaat
    30121 ttttacccgc acctcatcct gatgtttaat ttcaggaatg acgctttttg caacgcgcac
    30181 gataccatca gtatcattca ctactgattt cataaaaact cctgat
//
LOCUS       DAJTQQ010000041        30122 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000041 DAJTQQ010000000
VERSION     DAJTQQ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30122
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.041"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(13..519)
                     /gene="mug"
                     /locus_tag="OO309_002056"
                     /pseudo
     CDS             complement(13..519)
                     /gene="mug"
                     /locus_tag="OO309_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
     gene            644..719
                     /locus_tag="OO309_002057"
     tRNA            644..719
                     /locus_tag="OO309_002057"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:677..679,aa:Ile,seq:cat)
     gene            complement(773..1518)
                     /gene="nfeF"
                     /locus_tag="OO309_002058"
                     /pseudo
     CDS             complement(773..1518)
                     /gene="nfeF"
                     /locus_tag="OO309_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            1795..2418
                     /gene="nfeR"
                     /locus_tag="OO309_002059"
                     /gene_synonym="yqjI"
     CDS             1795..2418
                     /gene="nfeR"
                     /locus_tag="OO309_002059"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR5847201.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(2572..4092)
                     /gene="aer"
                     /locus_tag="OO309_002060"
                     /pseudo
     CDS             complement(2572..4092)
                     /gene="aer"
                     /locus_tag="OO309_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            4510..5889
                     /gene="ygjG"
                     /locus_tag="OO309_002061"
     CDS             4510..5889
                     /gene="ygjG"
                     /locus_tag="OO309_002061"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR5847202.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5931..6262)
                     /locus_tag="OO309_002062"
                     /pseudo
     CDS             complement(5931..6262)
                     /locus_tag="OO309_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            6480..7463
                     /gene="ebgR"
                     /locus_tag="OO309_002063"
     CDS             6480..7463
                     /gene="ebgR"
                     /locus_tag="OO309_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR5847203.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            7646..10732
                     /gene="ebgA"
                     /locus_tag="OO309_002064"
     CDS             7646..10732
                     /gene="ebgA"
                     /locus_tag="OO309_002064"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR5847204.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            10729..11178
                     /locus_tag="OO309_002065"
     CDS             10729..11178
                     /locus_tag="OO309_002065"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR5847205.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            11241..12674
                     /locus_tag="OO309_002066"
     CDS             11241..12674
                     /locus_tag="OO309_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5847206.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            12808..13128
                     /locus_tag="OO309_002067"
     CDS             12808..13128
                     /locus_tag="OO309_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5847207.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            13129..13482
                     /locus_tag="OO309_002068"
     CDS             13129..13482
                     /locus_tag="OO309_002068"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5847208.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            13460..13879
                     /locus_tag="OO309_002069"
     CDS             13460..13879
                     /locus_tag="OO309_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5847209.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            13896..16247
                     /gene="ygjK"
                     /locus_tag="OO309_002070"
     CDS             13896..16247
                     /gene="ygjK"
                     /locus_tag="OO309_002070"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR5847210.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            16473..18491
                     /gene="fadH"
                     /locus_tag="OO309_002071"
     CDS             16473..18491
                     /gene="fadH"
                     /locus_tag="OO309_002071"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR5847211.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18536..18952)
                     /gene="higA"
                     /locus_tag="OO309_002072"
     CDS             complement(18536..18952)
                     /gene="higA"
                     /locus_tag="OO309_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR5847212.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(18949..19263)
                     /gene="higB"
                     /locus_tag="OO309_002073"
     CDS             complement(18949..19263)
                     /gene="higB"
                     /locus_tag="OO309_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR5847213.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(19548..20684)
                     /gene="rlmG"
                     /locus_tag="OO309_002074"
     CDS             complement(19548..20684)
                     /gene="rlmG"
                     /locus_tag="OO309_002074"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR5847214.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            20769..21272
                     /locus_tag="OO309_002075"
     CDS             20769..21272
                     /locus_tag="OO309_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR5847215.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            21349..22041
                     /locus_tag="OO309_002076"
     CDS             21349..22041
                     /locus_tag="OO309_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR5847216.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            22120..23118
                     /locus_tag="OO309_002077"
     CDS             22120..23118
                     /locus_tag="OO309_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5847217.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            23389..24354
                     /gene="alx"
                     /locus_tag="OO309_002078"
     CDS             23389..24354
                     /gene="alx"
                     /locus_tag="OO309_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR5847218.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            24950..26194
                     /gene="sstT"
                     /locus_tag="OO309_002079"
     CDS             24950..26194
                     /gene="sstT"
                     /locus_tag="OO309_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR5847219.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(26199..26750)
                     /locus_tag="OO309_002080"
     CDS             complement(26199..26750)
                     /locus_tag="OO309_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR5847220.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(26833..28320)
                     /gene="uxaA"
                     /locus_tag="OO309_002081"
     CDS             complement(26833..28320)
                     /gene="uxaA"
                     /locus_tag="OO309_002081"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR5847221.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILSTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            complement(28335..29747)
                     /gene="uxaC"
                     /locus_tag="OO309_002082"
     CDS             complement(28335..29747)
                     /gene="uxaC"
                     /locus_tag="OO309_002082"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR5847222.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
BASE COUNT         7116 a         7964 c         7818 g         7224 t
ORIGIN      
        1 cggtgacgtt ttttatcgcc cacgcactac cagcgcctgg tccagctcgc gatacgcttc
       61 aaccagtttc tccagtgaaa cgcgacttaa accgctggga tttggcagca cccaaatctg
      121 cgtcgaacca atggtgagcg tttgtttccc ccactgtgca ccgcgctagc tgaatccctg
      181 ttcatatgct tgtttgccca gaatagccaa cgcctgcggc tgataatctt caattttttc
      241 aatcagctta cgcccacctg cgtgcagctc ctgctttgaa acttcattgg cttgcaccgt
      301 tggacggtct accagtttgg tgatgccaca acgataatcc agcagatgct gtgcctcctg
      361 cggcttcaac tgacggtcgg taaacccggc ctgatatatc accttccaga agcgatttgc
      421 cggatgagca aagggaaaac cagtcccggc ggatgaaagc ccagggttga taccgcaaaa
      481 cacgacccgt aaccctggag ccaaaatatc ctcaaccata tttactcctt tctaacatca
      541 ccaggaaaag tataacggat tgaaaataca ttgtttataa aaacagcagg cgcgcggtaa
      601 tggctggatt gcggcacgga gttactttat aatccgctac catggcccct tagctcagtg
      661 gttagagcag gcgactcata atcgcttggt cgctggttca agtccagcag gggccaccag
      721 atatagcaaa ggctgacgag aaatcgtcag cctttttctt tttatatatc agttactttg
      781 cgtgccagta agccgctgca cgtacccgct gtgggtcata ctgttccgct tcaaagcggc
      841 ggcttaaatt cttaacgact ttaccttcgc cggttatcca gatgaagtaa tcatcggcag
      901 ggatttgcat ctgcgccaga cgcgcatcta ccgcctgctc atcatgtgcc agccattcga
      961 tattaaaacc atcaaggtgc gcgagataat cctgacaggc gttatcccgc acgctaacca
     1021 gcgcactaac ttgcggttta acggcaagtt tgctcaacgt ttccaggcgg cggcgcagtg
     1081 cgggcattcc ggattcatcg cagacataca gctgatacgc gtaatcttcc ggcaccacca
     1141 gcgaaccgcg cggacctgcc accgtaagtt tatcgcccag ccgctggcga ccccaccgtc
     1201 gtgaataaag aaatcgatcg ccagttcatg gcgtagttcg tcatacagtg gcgtatagtc
     1261 acgcgacggt gggcgtggtc cttccggcca gacgatgctc tcttccgtta ccgttggcgg
     1321 cacaaagtga gcgtcaggtt gaggaaagaa gagtttgctg tgatcgtcaa agccgcgcga
     1381 tgtaaaaccg tccaacgcct cgccgccaag gacaatgcgc tgaaaaccgg cgctgatgcg
     1441 ctcaacgcgt agcacagtca gttcacggaa gcgcagatca ttgcgaacgc gctgcgggta
     1501 gcggggggtg ttattcattg ttatcgcctt cgtgatggta atcagatata tctaaataaa
     1561 attcgcaaat gataatgatt gttaatcatg ataaatgcaa gcgatttgta gaactgatat
     1621 gtctgaagta tgtgaaggca ttcagtgcca tagcaatgct gccggatgcg gcgtgaacgt
     1681 cttatccggc ctagatgtga ggtgcttcca cgtttaaaaa tcatttttgt acttgcagta
     1741 atgctcacat cagatataaa ttagatatat ctaatcaagc aaaaggaggc tgatatgagc
     1801 catcatcacg aagggtgttg taaacatgaa ggccagccac gccatgaggg ctgctgcaaa
     1861 ggtgagaagt cagaacacga gcactgcgga cacggtcacc agcatgaaca cggtcaatgt
     1921 tgcggtggtc gccacggtcg cggcggcggt cgtcggcaac gtttctttgg tcacggtgaa
     1981 ttgcgtctgg tgattctgga tattctctcg cgcgatgaca gccacggtta cgaattgatt
     2041 aaagcgattg agaatctaac ccaggggaat tacaccccaa gcccgggcgt catctacccg
     2101 acgctggatt ttctgcagga gcagtcgctg attaccatcc gcgaagagga aggtggtaag
     2161 aagcagattg cgctgaccga acaaggcgcg cagtggctgg aagaaaaccg cgaacaagtg
     2221 gagatgattg aagaacgcat caaagcgcgt tgcgttggcg cggcgctgcg ccagaacccg
     2281 caaatgaagc gggcgctgga taattttaaa gcggtgctgg atttacgcgt caaccagagc
     2341 gatatcagtg atgcacaaat aaaaaagatc attgcggtga tcgaccgcgc cgcttttgat
     2401 attacgcaac tggattaatc gccgcatccg ccagtggcgc ggtgcaattg ccggatgcga
     2461 cgcttgacgc gccttatccg gcctacaccc gctacacacc ccgcaggcct gataagatgc
     2521 gccagcatcg catcaggcat tgtgctccaa ccgccggatc cggcataccg attaatgcag
     2581 caccgtcacc gcgtcttcca gtcggctggc gcggtgtttc accatcgccg acacctgcgc
     2641 actctcttcc accagctcgg catttttctg ggtgatcagg ttaagctcat ccactgcacg
     2701 ggtcaggctg gaaagcccat cggcctgttc cagcgttgaa tggctaatct gggcgatcaa
     2761 ctgggtgacg tttttcacct gtgccacaat atcttccatc gtccgtccgg cggcgtgtac
     2821 ctgctgcgaa ccggattgca ccttatcagc actggcatca atcagcttgc gaatatcgtt
     2881 ggcagcatta gcgctgcggc tggctaaatg acgcacttcc cctgccacca ctgcaaaacc
     2941 tttgccctgt tcgccggcac gcgccgcttc caccgccgca ttcagggcca gaatattggt
     3001 ctgaaacgca atatcgttaa tcagcgaagt aatggtgcca atgcgctagg tactgtcggc
     3061 gatatcgtcc attgtcttga tcaccgtggt catcgcctcc ccaccctgca ctgccgcatt
     3121 actggcggtg atagaaagtt tatcggcagc cgacgccgtg gcagagttct gtttcaccga
     3181 cgccgccatt tggttcatgg tcgccaccgt ttgctgaacg ttatcaactg tctgctgggt
     3241 atgttcgttc agttcatcgg tgcctttcgc cagcgtctca ctgccatttc tgacactgga
     3301 cacctggctt gagacatcgt taattagcca acggcacatc aggccaagct gccctaccgc
     3361 gcgtaatgtc agccccagct catcgctgcg attcaggtgc tcaacactat tacgctctcc
     3421 ggtcgccacc ttcagtgcct gacgggcgac attttctatc gggcgcacaa tctgccattc
     3481 aaaacaagcg cttgccaaca ataccactaa cacacagagg aaatacgtca ccaccggggc
     3541 agcaacaaac caaagcatgg ccgccagcaa gataaacatc agggtcatca ctccacgcgt
     3601 ccgccagcga agcggtaatg aaggcagttt acccagccag cctttacgca ccaccaggcc
     3661 tttatgaata cgcttactgg tacgtccgga gttcaacgct ttgtacagcg gctccaccgc
     3721 cgcaatctct tcatccgtcg cccgggtacg aatcgacata tagccactga tttttccctc
     3781 gcgcaccatc ggtaccgcat tggcccgcac ccaataatgg tcgccatttt tgcggcgatt
     3841 tttcacgatg ccgctccagg gctccccttt tttcagggtg aaccacatat ccgcaaacgc
     3901 cgcttttggc atatccgggt gacgcaccat gttgtgcggc tgcccttgta actcttgcaa
     3961 ggtaaagccg ctcacctgca caaaagtgtc attagcatga gtgatatagc tttgcagatc
     4021 ggtagtggac atcagagtgg tatcgtccgc cagcggggta ttttgctggg tgacatacgg
     4081 atgagaagac atgttcgcgt cctgtgcagg ttatatggtt gttaacttct tgtcagagtt
     4141 tatgtcggcc ccgctgcggt tatctttaac cgattaattt gatttagatc gcaatttgcg
     4201 attaaagcac aaatctaatt ctttgattta aaatactttc actctgttac tatacgaaaa
     4261 cgttaattat cttgcccaaa aatcaggcaa ttattgccct gaaaacgtgc atttgcgcag
     4321 caatcatcaa atccataccc gacaaaaacc gtgcaaaata acaacaaatg ttaacggata
     4381 gcattaaata ttgcacaaat gataaccgca tttgtgttta tcccgatttt cgcgatcgca
     4441 gccggagtgg cgcaatccct gcaatactta aatcggtatc atgtgatacg cgagcctccg
     4501 gagcatattt tgaacaggtt accttcgagc gcatcggctt tagcgtgcag cgcccacgcc
     4561 ctgaatctca ttgagaagcg aacgctggat catgaggaga tgaaagcact taaccgagag
     4621 gtgattgaat acttcaaaga gcatgtcaat ccggggtttt tagagtatcg caaatctgtt
     4681 accgccggcg gggattacgg agccgtagag tggcaagcgg gaggtttaaa tacgcttgtc
     4741 gacacccagg gacaggagtt tatcgactgc ctgggaggtt ttggaatttt caacgtgggg
     4801 caccgtaatc cagttgtggt ttccgccgta cagaatcaac ttgcgaaaca accactgcac
     4861 agccaggagc tactcgatcc gttacgggct atgctggcga aaacccttgc tgcgctaacg
     4921 cccggtaaac tgaaatacag cttcttctgt aatagcggca ccgagtccgt tgaagcggcg
     4981 ctgaagctgg cgaaggctta tcagtcgccg cgcggcaaat ttacttttat tgccaccagc
     5041 ggcgtgttcc acggtaaatc acttggcgcg ctgtcggcca cggcgaaatc gaccttccgc
     5101 aaaccgttta tgccactgct gccagggttc cgtcatgtgc cgtttggcaa tatcgaagcc
     5161 atgcgcacgg ctcttaatga gtgcaaaaaa accggtgatg atgtggctgc ggtgatcctc
     5221 gaaccgattc agggcgaagg tggcgtaatt ctgccgccgc cgggctatct caccgccgtg
     5281 cgtaagctgt gcgatgagtt cggcgcactg atgatcctcg atgaagtaca aacgggcatg
     5341 gggcgcacgg gcaagatgtt tgcctgcgaa catgagaacg ttcagccgga cattctgtgt
     5401 ctggcaaaag ccctcggcgg cggcgtgatg ccgattggcg cgaccatcgc cactgaagag
     5461 gtgttttcag ttctgttcga caacccattc ctgcatacca ccacctttgg cggcaacccg
     5521 ctggcctgtg cggcggcgct ggcgaccatc aatgtgttgc tggagcagaa cttaccggct
     5581 caggctgagc aaaaaggcga tatgttgctg gacggtttcc gtcaactggc gcgggaatat
     5641 cccgatctgg tacaggaagc gcgtggtaaa gggatgttga tggcgattga gtttgttgat
     5701 aacgaaatcg gctataactt tgccagcgag atgttccgcc agcgcgtact ggtggccgga
     5761 acgctcaata acgccaaaac gatccgcatt gaaccgccac tgacactgac cattgaacag
     5821 tgtgaactgg tgatcaaagc ggcgcgtaag gcgctggcgg ccatgcgagt aagtgtcgaa
     5881 gaagcgtaat atcaaatcgg atggcgatac gacgtcgcca tccgatcttt ttatacgacg
     5941 cgtacgcccg caggcatcat ccgctccggc gttaacaaca cgctttcact gccgtcatcg
     6001 gtttccgcac acaacagcat gcattccgac gtttcaccac gcattttcgc tttttgcaga
     6061 ttgcataaga ccaccaccgt tttccccatc agctcctctt cgctgtagta cggcaccagg
     6121 ctggtcacgg tttgcagtgt tttttcccca catcaacctg tacgatgtac agcttgtcgg
     6181 cgttttcatg gcgtttcact tccacaatct ttccgacgcg catttccaga cgtgcaaaat
     6241 cagcgtaagc cacggtttcc attgcttcct cccttcgagt aaaattttac taaactatag
     6301 aaaagttttt ctcaatcctg taggctaaaa atggagaatg caggcgtgat cacattccta
     6361 agccgctgtg ttaccgttac agcgtcaaag aaacgcgctt tatttactga aaacaggtga
     6421 cccgataagc acttcctcta caatgggggc gcacatcagg gaaagtaaaa aaggtaaaca
     6481 tggcaacatt aaaagacatc gcaatcgaag ctggcgtatc cctggcgaca gtatccaggg
     6541 tcttaaatga cgatccgaca ttgaatgtga aagaagagac gaaacatcgc attctcgaga
     6601 tcgccgaaaa gctggagtac aagaccagta gcgcccgtaa actacagaca ggcgcagtca
     6661 accaacacca tattctggct atctacagct accagcagga gctggagatc aacgatcctt
     6721 actatctggc gatccgccac ggcattgaaa ctcagtgcga aaagctggcg atcgaactga
     6781 ccaactgtta tgaacacaac ggattaccag acattaaaaa cgtcaccggt attttaattg
     6841 tcggcaaacc cacgcccgcc ctgcgcgccg ctgccagcgc gttgaccgac aatatctgtt
     6901 ttatcgactt tcacgaaccc ggcagcggtt acgatgcggt ggatatcgat ctggcacgca
     6961 tcagtaaaga aatcatcgac ttctatatca accagggcgt taatcgtatt ggttttattg
     7021 gcggtgaaga tgagcctggc aaggcggata ttcgtgaggt cgcctttgcg gaatatggcc
     7081 gactgaaaca agtggtacgc gaagaggata tctggcgcgg cggtttttcc agttcgtcgg
     7141 gttatgaact ggcaaaacaa atgctggcgc gggaagacta tccgaaggca ctgtttgttg
     7201 cttccgattc cattgctatc ggcgtactgc gggcaattca tgaacgtggc ctgaacatcc
     7261 cacaagatat ttcgcttatc agcgttaacg atatccccac cgcgcgattt acctttccgc
     7321 cgctctccac cgtgcgcatc cattccgaga tgatgggaag tcagggcgtt aacctggtgt
     7381 atgaaaaagc ccgcgatggt cgcgcgctgc cgctgctagt cttcgttccc agcaaattaa
     7441 aactgcgcgg cacgacccgt taaatcccct tacacactgt tcggcaatca tttttgccgg
     7501 acagtgctgc cgtttatttt cgtgatccag ttaaagtaaa tgcatttacc tgctgctttt
     7561 tagtaaaaat tttactaaac ccccagcaat tacacaaact accatcacca tgaatggttc
     7621 cgatttctct ctaccgggag gccctatgaa tcgctgggaa aacattcagc tcacccacga
     7681 aaaccgactt gcgccgcgtg cgtacttttt ttcatatgat tctgttgcgc aagcgcgtac
     7741 ctttgcccgc gaaaccagca gcctgtttct gcccttaagc agtcagtgga atttccactt
     7801 ttttgaccat ccgctgcaag taccagaagc cttcacctct gagttaatgg ctgactgggg
     7861 gcatattacc gtccccgcca tgtggcaaat ggaaggtcac ggcaaactgc aatataccga
     7921 cgaaggtttt ccgttcccca tcgatgtgcc gtttgtcccc agcgataacc caaccggtgc
     7981 ctatcaacgt attttcaccc tcagcgacgg ctggcagggt aaacagacgc tgattaaatt
     8041 tgacggcgtc gaaacctatt ttgaagtcta cgttaacggt cagtatgtgg gtttcagcaa
     8101 gggcagtcgc ctgaccgcag agtttgacat cagcgcgatg gttaaaaccg gcgacaacct
     8161 gttgtgtgtg cgcgtgatgc agtgggcgga ctctacctac gtggaagacc aggatatgtg
     8221 gtggtcggcg gggatcttcc gcgatgttta tctggtcgga aaacacctaa cgcatattaa
     8281 cgatttcact gtgcgtaccg actttgacga agcctattgc gatgccacgc tttcctgcga
     8341 agtggtgctg gaaaatctcg ccgcctcccc tgtcgtcacg acgctggaat ataccctgtt
     8401 tgatggcgaa cgcgtggtgc acagcagcgc cattgatcat ttggcaattg aaaaactgac
     8461 cagcgccagc tttgctttta ctgtcgaaca gccgcagcaa tggtcagcag aatcccctta
     8521 tctttaccat ctggtcatga cgctgaaaga cgccaacggc aacgttctgg aagtggtgcc
     8581 acaacgcgtt ggcttccgtg atatcaaagt gcgcgacggt ctgttctgga tcaataaccg
     8641 ttatgtgatg ctgcacggcg tcaaccgtca cgacaacgat catcgcaaag gccgcgccgt
     8701 tggaatggat cgcgtcgaga aagatctcca gttgatgaag cagcacaata tcaactccgt
     8761 gcgtaccgct cactacccga acgatccgcg tttttacgaa ctgtgtgata tctacggcct
     8821 gtttgtgatg gcggaaaccg acgtcgaatc gcacggcttt gctaatgtcg gcgatatcag
     8881 ccgtattacc gacgatccgc agtgggaaaa agtctacgtc gagcgcattg ttcgccatat
     8941 ccacgcacag aaaaaccatc cgtcgatcat catctggtcg ctgggcaatg aatccggcta
     9001 tggctgtaac atccgcgcga tgtaccatgc ggcgaaagcg ctggatgaca cgcgactggt
     9061 tcattacgaa gaagatcgcg atgctgaagt ggtcgatatt atttccacca tgtacacccg
     9121 cgtgccgctg atgaatgagt ttggtgaata cccgcatccg aagccgcgca tcatctgtga
     9181 atatgctcat gcgatgggga acggaccggg cgggctgacg gagtaccaga acgtcttcta
     9241 taagcacgat tgcattcagg gtcattatgt ctgggagtgg tgcgaccacg ggatccaggc
     9301 acaggacgac cacggcaatg tctggtataa attcggcggc gactacggcg actatcccaa
     9361 caactataac ttctgtcttg atggtttgat ctattccgat cagacgccgg gatcgggcct
     9421 gaaagagtac aaacaggtta tcgcgccggt aaaaatccac gcgctgactc gcggcgagtt
     9481 gaaagtcgaa aataaactgt ggtttaccac gcttgatgac tacaccctgc acgcagaggt
     9541 gcgcgccgaa ggtgaaacgc tcgcgacgca gcagattaaa ctgcgcgacg ttgcgccgaa
     9601 cagcgaagcc cccttgcaga tcacgctgcc gcagctggac gcccgcgaag cgttcctcaa
     9661 cattacggtg accaaagatt cccgcacccg ctacagcgaa gccgggcatt ctatcgccac
     9721 ttatcagttc ccgctgaagg aaaacaccgc gcagccagtg cctttcgcac caaataatgc
     9781 gcgtccgctg acgctggaag acgatcgttt gagctgcacc gttcgcggct acaacttcgc
     9841 gatcaccttc tcaaaaatga gtggcaaacc gacatcctgg caggtaaatg gcgagtcgct
     9901 gctgacccgc gagccaaaga tcaacttctt caagccgatg atcgacaacc acaagcagga
     9961 gtacgaaggg ttgtggcagc cgaatcattt gcagatcatg caggaacatc tgcgcgactt
    10021 tgccgtagaa cagagcgatg gtgaagtgtt gatcatcagc cgcacagtta ttgcaccgcc
    10081 ggtgtttgac ttcgggatgc gctgtaccta catctggcgc atcgctgccg atggccaggt
    10141 taacgtggcg ctttccggcg agcgttacgg cgactatccg cacatcattc cgtgcatcgg
    10201 tttcaccatg gggattaacg gcgaatacga tcaggtggcg tattacggtc gtggaccggg
    10261 cgaaaactac gccgacagcc agcaggctaa catcatcgat atctggcgca gcaccgtcga
    10321 tgccatgttc gagaactatc ccttcccgca gaacaacggc aaccgtcagc atgtccgctg
    10381 gacggcactg actaaccgcc acggcaacgg tctgctggtg gttccgcagc gcccaattaa
    10441 cttcagcgcc tggcactata cccaggaaaa catccacgct gcccagcact gtaacgagct
    10501 gcagcgcagt gatgacatca ccctgaacct cgaccaccag ctacttggcc tcggctccaa
    10561 ctcctggggc agcgaggtgc tggactcctg gcgcgtctgg ttccgtgact tcagctacgg
    10621 ctttacgttg ctgccggttt ctggcggaga agctaccgcg caaagcctgg cgtcgtatga
    10681 gttcggcgca gggttctttt ccacgaattt gcacagcgag aataagcaat gaggatcatc
    10741 gataacttag aacagttccg ccagatttac gcctctggca agaagtggca acgctgcgtt
    10801 gaagcgattg aaaatatcga caacattcag cctggcgtcg cccactccat cggtgattca
    10861 ttgacctacc gtgtggaaaa tgactccgcg accgatgcgc tatttaccgg gcatcgacgc
    10921 tattttgaag tgcattacta cctgcaaggg cagcaaaaaa ttgaatatgc gccgaaagag
    10981 acattacagg tagtggaata ttatcgtgat gaaactgacc gtgaatattt aaaaggctgc
    11041 ggagaaaccg tagaggtcca cgaagggcaa atcgttattt gcgatatcca tgaagcgtat
    11101 cggtttatct gcaataacgc ggtcaaaaaa gtggttctca aagtcaccat cgaagatggt
    11161 tatttccata acaaataaca actacggcgg caaaaggagt ttgccgccac cgctacccta
    11221 cacattttcg gagatgtgtt atgtctgata ccaaacgtaa tacaatcggc aaattcggct
    11281 tgctctcgct gacttttgcc gccgtttaca gctttaacaa cgttatcaat aataatattg
    11341 agcttggact ggcctcggca ccgatgtttt tcctcgcgac gattttttat tttattccct
    11401 tctgtctgat catcgcagaa tttgtttcgt taaataaaaa ctcagaagcc ggtgtctacg
    11461 cgtgggtaaa aagttcgctg ggcggacgtt gggcatttat tactgcctac acttactggt
    11521 tcgtaaatct gttctttttc acctcgctgt tgccgcgcgt tattgcttat gcttcgtatg
    11581 ccttcctcgg ctacgaatat attatgacgc cggttgccac caccgttatc agtatggtgc
    11641 tgttcgcctt ctccacctgg gtttccacca acggggcgaa aatgctgggg ccaattacct
    11701 ccgtcacttc aacgctgatg ctgctgttaa cgctctccta cattttactg gcaggtacgg
    11761 cactggttgg cggcgtacag cctgccgatc cgatcaccgt tgacgcgatg atcccgaact
    11821 tcaactgggc gttcctcggc gttaccacct ggatctttat ggccgcaggt ggcgcggagt
    11881 ccgtcgcggt gtacgttaac gacgtcaaag gcggttcgaa atcgttcgtt aaagtgatca
    11941 tcctcgccgg gatttttatc ggcgtactgt attccgtctc ctcggtgctg attaacgtct
    12001 tcgtcagcag caaagagttg aaatttaccg gcggatcggt gcaggtattc cacggcatgg
    12061 cggcgtattt tggtctgccg gaagcgttga tgaatcgctt tgtcggtctg gtgtccttta
    12121 ccgcaatgtt cggttccctg ctgatgtgga ccgcaacgcc ggtgaaaatt ttcttctccg
    12181 aaatcccgga aggcatcttt ggtaagaaaa ccgtcgaact taacgaaaac ggcgttccgg
    12241 cgcgcgcagc atggatccag tttctgatcg tcatcccgct gatgattatc ccgatgctcg
    12301 gttccaatac cgtgcaggat ctgatgaata ccattattaa tatgaccgcc gcagcgtcca
    12361 tgcttccgcc gttattcatc atgctggctt acctgaattt acgcgccaaa ttagatcacc
    12421 tgccacgcga tttccgtatg ggctcccgcc gcaccggtat tatcgttgtt tcaatgctga
    12481 ttgcgatatt tgccgtaggg tttgtcgctt cgacattccc gactggcgcg aatattctga
    12541 ccatcatttt ttataacgtc ggcggtattg ttatcttcct cggctttgcg tggtggaaat
    12601 acagcaaata tataaaggga ttaacggctg aagagcgcca tattgaagcg acgccagcca
    12661 gcaatgttga ttaagcagac taataaaaaa tgagcaggca ttttccctct tcctggatga
    12721 ggagagggaa aatagtttct gcctttatat ttttataatt acaacgataa aaggctgtac
    12781 tttttcttta gctcatggat taacacaatg aaattaatca ctgcaccatg cagagcatta
    12841 cttgctctgc cgttttgcta cgccttttct gcggcaggag aagaagcacg tccggcagaa
    12901 catgacgaca caaaaacacc cgcaattacc tcgacatctt ctccttcatt tcgtttttac
    12961 ggcgaattag ggcttggtgg atatatggat ttagagggtg agaataaaca taaatacagc
    13021 gacggcacct atattgaagg tggcctggag atgaagtacg gctcctggtt cggcctgatt
    13081 tacggcgaag gctggaccgt gcaggccgac cacgacggta atgcctaggt gcctgaccat
    13141 agctggggtg gtttcgaagg tgggattaac cgtttctatg gcggttatcg taccaatgat
    13201 ggcaccgaaa tcatgctcag tctgcgtcag gattcctcgc tggatgacct gcaatggtgg
    13261 ggcgacttca cacccgattt gggctacgtc attcccaata cccgcgacat tatgaccgcg
    13321 ctgaaggtac agaacttaag cggcaacttt cgttatagcg tcaccgccac acctgccgga
    13381 catcatgacg aaagcaaagc ctggctacat tttggcaagt acgatcgcta cgacgacaaa
    13441 tacacctatc cggcaatgga tgaacggtta catccagtat gaccttgccg aaggcatcac
    13501 ctggatgaat ggtctggaaa ttaccgacgg cacaggacaa ctctatctca cgggcctgtt
    13561 tacccctaac tttgccgctc gcgcctggca ccataccgga cgtgccgacg ggctggacgt
    13621 accgggaagt gaaagcggga tgatggtgag cgccatgtat gaagcgttaa aaggcgttta
    13681 tctctccacc gcttacacct acgccaaaca tcgccctgac cacgctgacg atgaaaccac
    13741 ctctttcatg cagtttggta tctggtacga atatggcggc ggacgtttcg ccacggcttt
    13801 tgatagccgc ttctacatga aaaatgcctc taacgatccc agcgaccaaa tcttcctgat
    13861 gcaatatttc tactggtaat aaggactgta atctcatgaa aatcaaaact attttaacgc
    13921 cagtaacctg cgctctgctg ataagttttt ccgctcatgc cgctaacgcc gacaattata
    13981 aaaacgtgat taaccgtact ggtgcgccgc agtacatgaa ggattacgat tacgacgatc
    14041 accagcgttt taatccgttt ttcgatctcg gagcctggca tggtcatctg ttgccagacg
    14101 gcccgaacac catgggcggc tttccgggcg ttgcgctgct gacggaagaa tacatcaact
    14161 ttatggccag caatttcgac cgcctgactg tctggcaaga cggcaagaaa gtcaacttca
    14221 cgctggaggc atacagtatt cccggcgcgc tggtgcaaaa actgacagca aaagatgtgc
    14281 aggtcgaaat gactctgcgc ttcgccacgc cgcgcacgtc actactggaa actaaaatca
    14341 tcagcaataa accgctggat ctggtgtggg atggcgaact gctggaaaaa ctggaagcga
    14401 aagaagggaa accgctttcc gataaaacca ttgctggcga ataccctgac tatcagcgca
    14461 aaatcagcgc cacccgtgat ggcctgaaag tcacctttgg caaagtgcgc gccacctggg
    14521 atctgctgac ctccggcgaa tcggaatatc aggtgcataa atccctgccg gtgcagactg
    14581 aaatcaacgg taatcgcttt accagtaagg cgcatatcaa cggttcgacc acgctctaca
    14641 ccacctattc ccatctgctg accgctcagg aagttagcaa agagcaaatg cagatccgcg
    14701 atattctggc gcgtccggcg ttttatctca ccgcctcgca gcaacgctgg gaagaatacc
    14761 tgaagaaagg gttaaccaat ccggatgcga cgccggaaca gacgcgcgtc gcggtgaaag
    14821 cgatcgaaac gctcaacggt aactggcgct caccgggcgg tgcggtgaat tataacaccg
    14881 tcacgccgtc ggtgaccgga cgctggttct ccggcaatca gacctggccg tgggatacct
    14941 ggaagcaggc gtttgcgatg gcgcatttca atccggatat cgccaaagag aatatccgcg
    15001 cggtcttctc ctggcagatc cagcctggcg acagcgtgcg tccgcaggat gtgggctttg
    15061 tccccgacct gatagcgtgg aaccttagcc ccgagcgtgg tggcgatggc ggcaactgga
    15121 acgaacgtaa taccaaaccc agccttgccg cctggtcggt gatggaagta tacaacgtca
    15181 cccaggataa agcctggttg gcagagatgt acccgaaact ggtggcctat cacgactggt
    15241 ggttacgtaa ccgcgatcat aacggcaacg gggtgccgga atatggcgcg acccgcgaca
    15301 aagcccacaa caccgagagc ggcgagatgc tgtttacggt gaaaaaaggc gataaagaag
    15361 agacgcagtc tgggctgaac aactacgccc gcgtggtgga gaaaggccag tacgacagtc
    15421 tggaaattcc ggcacaggtc gctgcgtcgt gggaatcggg gcgtgatgat gccgccgtct
    15481 ttggttttat cgacaaagaa cagttggata aatatgtcgc taatggcggc aaacgtagcg
    15541 actggacggt gaaattcgcc gaaaaccgca gtcaggacgg aacgttgctg ggctactcgc
    15601 tattgcagga gtcggtggat caggccagct atatgtacag cgataaccat tatctggcgg
    15661 agatggcaac catcctcggt aagccagaag aggccaaacg ctatcgccag ttggcacagc
    15721 agctcgcgga ctacatcaac acctgtatgt tcgacccgac tacccagttc tactatgacg
    15781 tacgtattga agataaacca ctggcgaacg gctgcgcggg caaaccgatt gttgagcgcg
    15841 gtaaagggcc ggaaggctgg tcgccgctgt ttaacggtgc ggcaacgcag gccaatgccg
    15901 acgcggtggt gaaggtgatg ctcgatccta aagagttcaa cacatttgtt ccgctgggaa
    15961 cggcggcgtt aaccaatccg gcttttggcg ctgatatcta ctggcgcggg cgcgtatggg
    16021 tggatcagtt ctggtttggt ctgaaaggga tggagcgtta cggttatcgt gatgatgccc
    16081 tgaagctggc ggatacgttc ttcaggcacg ccaaaggatt aaccgccgat ggcccaattc
    16141 aggagaatta caacccgctg acaggcgcgc agcaaggcgc accaaatttc tcctggagtg
    16201 ccgctcattt gtatatgttg tataacgatt ttttccgtaa gcagtaattt gcaacggctg
    16261 gcggatgcgg cgtgaacgcc ttatccgccc tacatgtgca atcccccaag ccggataaaa
    16321 cacggcaagc gtcgcatccg gcgttaccac cgggcgtatt ctttttgaat cccatcacaa
    16381 accccgcact ccccttttcc cttttctcca tcgacggcta aattagaact catccgacta
    16441 cataacaatt attttacata ctggacaatt ttatgagcta cccgtcgctg ttcgccccgc
    16501 tggatttagg ttttaccacg ttaaaaaacc gcgtgttgat gggttcaatg cacaccgggc
    16561 tggaggaata cccggacggt gctgaacggc tggctgcgtt ttatgccgaa cgcgcccgtc
    16621 acggcgtggc gctgattgtc agcggcggca ttgcaccaga tttaacaggc gttggcatgg
    16681 aaggtggcgc aatgctcaac gacgccagcc agatcccaca ccatcgcacc attaccgaag
    16741 ccgtacatca ggaaggcggc aaaatcgccc tgcaaatttt gcataccggg cgctacagct
    16801 accaaccgca tctggtcgca ccgtccgcat tgcaggcccc catcaaccgt ttcgttcctc
    16861 acgaactcac ccatgaagag atcctgcaac tgatcgacga tttcgcccgc tgcgcgcaac
    16921 tggcgcggga ggccggatac gatggcgtcg aagtgatggg ctctgaaggg tatttgatca
    16981 acgaatttct gacgctgcgc accaatcagc gtagtgacca gtggggcggc gattaccgca
    17041 accggatgcg atttgccgta gaagtagtgc gtacggtgcg cgaacgcgtc ggcaacgact
    17101 tcattattat ttaccggttg tcgatgctcg acctagtcga aggcggcggc acttttgccg
    17161 aaacggtaga actggcgcag gccattgaag cggcgggcgc gaccattatc aacaccggaa
    17221 taggctggca cgaagcgcgg attccgacca tcgccacgcc cgtgccgcgc ggcgcattta
    17281 gctgggtcac gcgcaaactg aaaggccacg tctcgctgcc gctggtaacc accaaccgga
    17341 ttaacgatcc gcaggttgcc gacgatattc tctcgcgcgg cgatgccgat atagtatcga
    17401 tggcgcgacc gtttctcgcg gatgcggaac tgctgtcaaa agcgcaatca gggcgagccg
    17461 atgagatcaa cacttgtatt ggctgcaacc agggctgcct cgatcagatc ttcgttggca
    17521 aagtcacctc gtgcctggtg aatcctcgcg cctgccacga aaccaaaatg ccaatccttc
    17581 ccgccgtgca gaaaaaaaat ctggcggtgg tcggcgctgg acctgctggg ctggcgtttg
    17641 ccattaacgc ggcggcgcgt gggcatcagg taacattgtt tgatgcgcac agcgagatcg
    17701 gcgggcagtt taatatcgcc aaacagatcc ccggcaaaga ggagttttac gaaacgctgc
    17761 gctactaccg ccggatgatc gaagtgacgg gcgtgacgct gaaactcaat cacaccgtga
    17821 cggcggatca gttacaggcg ttcgatgaaa cgatcctcgc cagtgggatc gtgccgcgtg
    17881 ttccgcctat cgacgggatc gatcatccga aggtattgag ttatctcgat gtactgcgcg
    17941 acaaagcgcc ggtaggcaac aaggttgcca tcatcggttg tggcgggatt ggttttgata
    18001 cggcgatgta tttaagtcag ccgggcgaat ccaccagcca gaatatcgcc aggttctgta
    18061 atgagtgggg gatcgacagc agcctacaac aggctggtgg cttaagcccg cagggaatgc
    18121 agatcccgcg tagcccacgg caaattgtga tgctccagcg caaagccagc aaaccgggac
    18181 aggggttagg caaaaccacc ggctggatcc atcgcaccac cctgctctcg cggggtgtga
    18241 aaatgatccc aggcgtaagt tatcagaaga ttgacgatga cgggctgcat gtggtgatca
    18301 acggcgaaac gcaggtatta gcagtggaca atgtggtgat ctgcgcaggg caagagccaa
    18361 accgcgcgct ggcgcaaccg ctgattgata gcgggaaaac tgtgcattta attggcggct
    18421 gcgatgtggc tatggagctg gacgcacgac gggcaattgc ccagggaaca cggctggcgc
    18481 tggagattta aatcgttatt tgaagcgccg gatgcaacgc attcggcacg tcgcgttaat
    18541 caataaacaa ggcgggagaa atgccgaatc gcgttgccaa ttttttagcg tgttccagcg
    18601 ttaatttcct cttcccgctc aaaacgcgtg acaccataga tttactgcca atttccggca
    18661 gatcggaaag ggttaaacca tattgatcca taagggtacg aatcacggct ataccgccag
    18721 gcatggcttg agccatggca ttaaattccg caaattcggg cgctgattct tcccacgcgg
    18781 ttattttggc acacaccaga tccagcaagg ggttttcagg atcgttgagc agcagatgat
    18841 ctaccagttc cagcgcctgg gtgtattgtt cctcgttctg aatacccgcc agaaaaggtg
    18901 ccacagcagt tagcttttct cctgcttgca agatgtcggc aatcgcaatc attttttccc
    18961 cttagtacga tgaacagcgg taaagaaatc gtattcttta tgcgtcataa cttcacgtat
    19021 gtagcacttt tgcgattcaa aaaagaccat tgctacaaca cgtaattcat tgcccccaac
    19081 attgaaaaca taatgcttat ccagatattt gaagttatcc agagatggga atactgcttt
    19141 taatgactca ggttttttga aatatccctt agcaatcgtg ttccccagag ccaccaactc
    19201 cgttttatgt tgcgggtatt tttccgcagc atctttcaat gctttttgag ttatcaggtg
    19261 cattcttcat cacgtccgtt gacaaattgg caatatgata acatccgttg acagattggc
    19321 aacggatgaa tttatctgtg tgacggaaga ataaacgtag aaaaacgcta aacaagagaa
    19381 tgtagaggat ggtgacaggg attttttgtt ttatggaagc gcgattccag aactgagcag
    19441 acattgccgg atgcgacgct tgccgcgtct tatccggcct gggggtaacg tgctgaacgt
    19501 aggccggata aggcgtttac gccgcatccg gcaggtaact cccaatctta ccgacgacgt
    19561 cccagcttca ccgctttcag caccacaaat ttattattcg tcgcaatagt ggtgcagttg
    19621 ccgaaaattt tcttcagttt atggaagtaa tccagatggc ggttggcaac gatatacagc
    19681 tcgccgttga ttttcaggca gcggcgggcg tagtggaaca tctcccaggc gacgttatcg
    19741 gtcagcgcat gttgttggtg aaacggaggg ttgcagagca cagcattaaa gcggaaaggc
    19801 tccacgccgg agagcgcgtt gttgatcata aactcgctgc gatccaacgc ctctggcatg
    19861 ttggtttcaa cgttcaaacg gctggaagca accgccatcg gcgattcatc gacaaacacc
    19921 actttcgcct gcgggttttt atcaagcagc gtcagaccaa taacgccatt accgcaaccg
    19981 agatcgacaa tctccccttc gagattctct ggcagatgtt gcataaagaa gcgcgcgccg
    20041 atatcaagcc cggtgcggga gaagacattc gcatggttat ggatagtcca gtcagtacct
    20101 tccagcttcc agctaacggt ctgcggcgca tcggccagcg gcggttcatt gaacgtgcaa
    20161 ttaatcaggc gcgctttttt ccatgccagc gtggtggtgg tcgggccgag cactttttcg
    20221 aacagttcca gcgtggaagt gtgaatgtca cgggctttgg caccggcaat aatacgtgta
    20281 tccgacgtga ccactttgcg cagcgcacgc agttgctgtt ccagcaatgc cagtgttttc
    20341 ggcactttga tcagcaccac acccggctgt tgcgggtagt cggcggtgct gtcgagaaac
    20401 ttcacgctcg actcatcaat cccgtttagg cgtaaattct cgcgcgtcgc cagttcactg
    20461 atgtatgagt cgccaatgct gtacggctta tgttccgcca gcgcgcagct taacgcacca
    20521 aaggcatcat tcaggatcaa caccgggccg aggatttctg tgtcgtccaa ctgttgcagc
    20581 aaatattcat ctgccgcttc ccacgcctgt agcgggttaa cgtcatccgt cgccggaaaa
    20641 cgttgtagtg tcagtgaacg gaaaccgttg tctaagtggc tcatcggccc tcctgaatga
    20701 taaaatttcg acgttgcccc tgaaaagggt gcgtgagtat accctttttc tctttttcgt
    20761 gggcggttat gagcaatctt acttatcttc agggttatcc cgagcagctg ctttcgcagg
    20821 tgcgaacact gattaacgaa cagcgtctgg gcgatgtgct ggcaaaacgc tatccgggaa
    20881 cacacgacta cgccaccgat aaagctctct ggcaatatac tcaggatctg aaaaatcagt
    20941 ttctgcgtaa tgccccgccg atcaataaag tgatgtatga caataagatc catgtgctaa
    21001 aaaacgcgct cgggttacat accgctgttt ctcgtgtgca gggcagcaag ctgaaagcaa
    21061 aagcagagat ccgcgtcgct accgtgtttc gcaacgcgcc ggaaccgttt ttgcgcatga
    21121 tcgtggtgca cgagctggcg cacctgaagg agaaagagca taacaaagcg ttctatcaat
    21181 tgtgctgcca tatggagccg cagtaccacc agcttgagtt cgacacccgg ctgtggctaa
    21241 cgcagttgtc gcttgggcag gacaaaatct gaaaatgatg tcaggcgtta tcttttcttt
    21301 ctttagcgcc taaaatcgac ctcccccctt tcgttaagga taacgaccat gctgcgcgca
    21361 tttgcccgcc ttcttctccg tatttgtttt tctcgccgga ctctaaagat tgcctgctta
    21421 ctgttacttg tggcaggagc gaccattttt atcgccgatc gcgttatggt caacgccagc
    21481 aagcagctga cctggggcga tgtcaacgcc gtcccggcgc gtaacgtggg tttattgctg
    21541 ggggcgaggc cgggtaatcg ctactttacc cgacgtattg ataccgcagc agcgttgtac
    21601 cacgccggaa aagtgaaatg gctactggtg agtggcgata acggacgtaa aaattacgat
    21661 gaagcatcag gtatgcagca ggctttgatc gctaaaggcg taccggcgaa agtgatcttt
    21721 tgcgactatg ccggattctc aacgctggat tcggtagtgc gtgccaaaaa ggtgtttggg
    21781 gaaaaccata tcaccattat ctcgcaagaa tttcataacc aacgcaccat ctggctggca
    21841 aaacagtacg gcatcgacgc tatcgggttt aacgcgccag acctcaatat gaagcacggt
    21901 ttttatactc aactgcggga gaaactcgct cgtgtcagcg cggtgattga cgcaaaaatc
    21961 ctccatcgcc agccgaaata tctcgggcca tcggtcatga ttgggccgtt tagtgagcat
    22021 ggctgtccgg cgaaagaata atgcgtatct gcgcacgtcg aagatgaaaa aggcgtgcta
    22081 cattgacgac agaatccctt tatggagtat ccacgcgtta tgatacgttt cgctgtgatt
    22141 ggtacgaact ggatcactcg ccagttcgtc gaggccgccc atgagagcgg taaatacaag
    22201 ttaaccgccg tatattcccg cagccttgag caggctcagc acttcgccaa tgatttttct
    22261 gtcgagcatc tgtttacgtc gctggaagcg atggcggaaa gcgatgccat tgacgcggtg
    22321 tatattgcca gcccgaattc cctgcacttt tcccagacac aacttttcct tagccataaa
    22381 attcatgtga tttgcgaaaa accgctggcg tcgaatctgg cggaagtgga tgccgccatt
    22441 gcctgtgcgc gcgaaaatca ggtggtgctg tttgaggcat ttaaaaccgc ctgcctgccg
    22501 aactttcatt tgttgcgcca ggcgctgccg aaagcaggca aactgcgtaa agtctttttc
    22561 aactattgcc agtattcctc gcggtatcaa cgttacctgg acggtgagaa tcccaacacc
    22621 tttaatccgg cattctctaa cggttcaatt atggatatcg gcttttactg cctggcgtcg
    22681 gcggtggcgt tatttggtga gccgaaaagc gtgcaggcaa cggcgagttt gctggcaagc
    22741 ggcgttgacg cccacggcgt ggtggtgatg gattacggtg atttcagcgt caccttgcag
    22801 cactcaaagg tcagtgattc tgtcctggga agcgagattc agggcgaagc gggatcgctg
    22861 gtgattgaaa aactgtctga atgccagaaa gtctgctttg tgccgcgtgg cagccagatg
    22921 caggatctca cccagccgca gcatattaat accatgctct acgaagcaga gctgttcgct
    22981 accctggtgg atgagcatct ggtggatcat ccagggctgg cggtcagtcg catcacagcc
    23041 aaactgctga ccgagatccg ccgccaaact ggggtgattt ttccggcaga tagcgtaaaa
    23101 ctatacttgc caaagtaaaa cagtgtaaaa ggtatgtaac agaccattga ctgactgaat
    23161 ggtctgtcat actttgttac ctgcaaaggg gagtaacttc attgccggtc gatcgtcatt
    23221 acgatgagtg aaaaaccaca tccggtcacc gggcaacccg aaaggaatac gcagacgtat
    23281 tccttttttg ttgtaagtga gaccttgccg gaaggcgagg tctatgcata aaaagcagcg
    23341 gctgacgtct tccgacgttg gccgtttttt tatgtgtaag gaacttctat gaatactgtc
    23401 ggcacgccgt tgctatgggg cggattcgct gttgttgtcg ccattatgct ggctatcgac
    23461 ctgttgttgc aggggcgtcg tggggcacat gccatgacca tgaaacaggc tgcggcctgg
    23521 tcgctggtct gggtgacgct gtcgttactg tttaacgccg ctttctggtg gtatctggtg
    23581 caaaccgaag gtcgcgccgt tgccgatcca caggccctgg cctttctcac gggttatctg
    23641 attgagaaat cgctggcggt cgataacgtc tttgtctggc tgatgttgtt cagctatttc
    23701 tccgttccgg cggcattaca acgtcgcgtg ctggtgtatg gcgtgctcgg ggcgattgtt
    23761 ctgcgtacca tcatgatctt cacaggcagc tggctgattt cgcagttcga ctggatcctg
    23821 tatatcttcg gtgccttcct gctgtttacc ggcgtgaaga tggcgctcgc ccatgaagat
    23881 gaatcaggca ttggcgacaa gccgctggtg cgctggctac gcggtcattt gcgcatgacc
    23941 gacaccatcg acaacgagca tttctttgtg cgtaagaatg gattgctgta cgccacaccg
    24001 ctgatgctgg tactgattct ggtggaattg agcgacgtga tttttgccgt ggatagcatt
    24061 ccggctatct tcgccgtgac cactgacccg ttcattgtgc tgacctcaaa cctgtttgca
    24121 atcctcggcc tgcgtgcgat gtatttcctg ctggcgggcg tagcagagcg tttctcgatg
    24181 ctcaaatatg gcctggcggt gattctggtg tttatcggta tcaagatgct gattgtcgac
    24241 ttctaccata ttccaatcgc cgtctcgctg ggcgtggtgt ttggcattct ggtgatgacg
    24301 tttattatca acgcctgggt gaattatcgg catgataagc agcggggaga atgattcgct
    24361 gtatattaag ccattcctag agataactca ttccagacaa aggtgagcta ttaattatgt
    24421 aatcggctta tagctcttta caaaaagacc gcacgggtgt tggtggcgtc aaaatcgccg
    24481 aagcgttccc tggaggccgg ggcgagggcg catcttacag ggacgttacc ttgcgcccga
    24541 cccggtagcc gcaagggata ggctgaggga accgcgcagc ggcgattttg ttcgccagag
    24601 cctgggggtg cagggggcgg cggcgattgg ccgccccctg cgcgctccct gcgccagtga
    24661 ctttatatgg cataacacgt taaaggagcg caaccgttct catattccaa atagttttgc
    24721 ccatatgtta tctaatcagc cttagtagcc agtccttccc tctgtttatg ttaaataaat
    24781 gcaaccatta agttgcaaaa catgacctct ctttaaaatc cagcattttt cgcttcccga
    24841 agctgtaact ttccttatac tcgaccttgc aaacactttg ttacatcctg aaagatgcgt
    24901 cgacagaacg caccagggat gtgcgacaac acaatgaaag gatcgaaaaa tgactacgca
    24961 acgttcaccg gggctattcc ggcgtctggc tcatggcagc ctggtaaaac aaatcctggt
    25021 cggccttgtt ctggggattc ttctggcatg gatctcaaaa cccgcggcgg aagctgttgg
    25081 tctgttaggt actttgttcg tcggcgcact gaaagctgtt gcccccatcc tggtgttgat
    25141 gctggtaatg gcatctattg ctaaccacca gcacgggcag aaaaccaata tccgtcctat
    25201 tttgttcctc tatctgctgg gcaccttctc tgcagctctg gccgcagtag tcttcagctt
    25261 tgccttccct tctaccctgc acttgtccag tagcgcgggt gatatttcgc cgccgtcagg
    25321 cattgtcgaa gtgatgcgcg gactggtaat gagcatggtt tccaacccca tcgacgccct
    25381 gctgaaaggt aactacatcg ggattctggt gtgggcgatc ggcctcggct tcgcactgcg
    25441 tcacggtaac gagaccacca aaaacctggt caatgatatg tcgaatgccg ttacctttat
    25501 ggtgaaactg gtcattcgct tcgcaccgtt cggtattttt gggctggttt cttctaccct
    25561 ggcaaccacc ggtttctcca cactgtgggg ctacgcgcaa ctgctggtcg tgctggttgg
    25621 ctgtatgtta ctggtggcgc tggtggttaa cccattgctg gtgtggtgga aaattcgtcg
    25681 taacccgttc ccgctggtgc tgctgtgcct gcgcgaaagc ggtgtgtatg ccttcttcac
    25741 ccgcagctct gcagcgaaca ttccggtgaa tatggcgctg tgtgaaaagc tgaatctgga
    25801 tcgcgatacc tattcagttt ctattccgct gggagccacc atcaatatgg cgggcgcagc
    25861 aatcaccatt accgtgttga cgctggctgc ggttaatacg ctgggtattc cggtcgatct
    25921 gcccacagcg ctgctgttga gcgtagtggc ttctctgtgt gcctgtggcg catccggcgt
    25981 ggcggggggt tctttgctgc tgatcccact ggcctgtaat atgttcggta tttcgaacga
    26041 tatcgccatg caggtggttg ccgtcggctt tattatcggc gtattgcagg actcttgcga
    26101 aaccgcgctg aactcttcaa ctgacgtgct gttcactgcg gcagcttgcc aggcggaaga
    26161 cgatcgtctg gcaaatagcg ccctgcgtaa ttaattgttt aacccctttc gtctatggcg
    26221 ggaggggttt tctctacttt aaacggatca attccccttt tctgcatccg ccagaaacga
    26281 atgatattca ggccattcat aagcagaaaa ctaccctcaa tcatcgtgcc gcctatcgac
    26341 cccgcccaga agttgtgaat cacccagcaa cacgttgaaa accacatcac gcagcgcatg
    26401 gtgagccctt tacagcggaa cagcgcccag gtactgacaa tcgtaccgat aaccggcaat
    26461 agttcgacag gatgatggaa cttcgcgagg ccaattccgc cagtcagcac aataaaaatc
    26521 gccattaccc ataagctgcg cgtgcgtaag gtaatcaatg tacgaatggc attaaggatg
    26581 gcactggcac cagcgggata ggtgcccaga agaaaaaaat gtacgccaat aacggcgcta
    26641 tagaccgaaa gctgcttttt gaagcgacgt tcgtcacgat tgaaaaatgt tgtgatacca
    26701 atcagaaagg cgatgacacc cacgccctgg gccagccaat acgcggtcat gataaatcct
    26761 tagcaggtat ggaaaagcaa acggcgcttc acattatgaa acgccgtttt ttattaacaa
    26821 ctcatttcga ctttatagcg ttacgccgct tttgaagatc gccagttcgc ggaagtcgtt
    26881 acgctcgttg caggtttgct taccgttggc aaactcaacg atggtgtcga taaattcttc
    26941 cagcaactgc ggcatcgctt taccgtggat cagctgaccc gcgtcaaagt cgatccagtg
    27001 ttttttcttc gccgccagtt cactgttggt ggcgattttc accgtcggca caaatccacc
    27061 gtatggcgtg ccgcgaccag tactgaacag caccatatgg cagcccgcac ccgccagggc
    27121 gctggtcgct acggcatcgt tacccggcgc acttaacaag ttcagccctg gcgttttcag
    27181 acgctcgccg taacgcagca cgtcaaccac gacactggaa cccgctttct gggtacagcc
    27241 aagtgatttg tcttccagcg tggtgatacc gcccgctttg ttccccggtg atgggttttc
    27301 gtagatcggc tgatcatggg caataaagta ctgtttgaag tcgttgacca tggtgaccag
    27361 tttttcaaac gttgcttcgt cgcggcaatg gtccatcagc aactgctctg cgccaaacat
    27421 ctccggcact tcggtcagta cggtagtacc gccgttagca atcacgtagt cagagaaacg
    27481 ccccagcatc gggttagcag taataccaga aagaccgtca gaaccaccgc actccagacc
    27541 aaacttcagt tcgctgagtt tgcctggctc gcgtttatcg ttgcgcatca cgttatacag
    27601 ctgatgcaaa tgctcgatcc cggcttcgat ctcatcatcc tgctgttggc agatcatgaa
    27661 atgaacgcgt tcaggatcga tatcgcccaa cgtttcacgg aatgcggcaa cctggttgtt
    27721 ttcacagccc agaccaatca ccagcactgc gcccgcgttc gggtggcgca ccatgttttg
    27781 cagcatggtg cgggtgttga tgtgatcgtc gcccagttgt gagcagccat aggtgtggct
    27841 gaagaggaac acgccgtcgg taccttcggc gttgttggtc tctttcagga aacggttctg
    27901 gatttgccgc gcgatgccgt tgacacagcc cacggttgac aggatccaca gctcgttacg
    27961 caccccgaca tcgccgttag cgcgacgata gatctgcact tcaggatctg ccgcttgcgc
    28021 aggcagatcc tgaaaatcaa gttgatagcg atactgatcc agatcgctca gattcgtgcg
    28081 cgtattgtgg gcgtggacgt gttcccccgc cgcaatatcc gccaatgcat aaccaatcgg
    28141 caggccatat ttgatgacgt tggccccttt tgcgatatcc gttaacgcaa atttatgtcc
    28201 acgagcaaca tcctggcgca gcgtaacagt ctggttatcg acactgactt ctgtgccttc
    28261 agccaaatct gctaaagcga ccgcgacgtt atccagcgca tggatcttga tgtattgcat
    28321 atcaacccca gaccttagtt cagttcaatg gcgaagtagt cacgcgcatt gttaaagcaa
    28381 atatttttca ccatctcgcc cagcaggttg atgtccgccg gtgcttcgcc cgcttccacc
    28441 cagcgaccga tcatctggca cagaatgcgg cggaagtatt cgtgacgggt gtatgacagg
    28501 aagctacggc tgtcagtcag cataccgaca aagcggctca gcagaccgag ctgcgccagt
    28561 tgggtcatct gacgttccat accgtctttc tgatcgttaa accaccagcc ggaaccgaac
    28621 tgcattttgc ccggcatacc ttcgccctgg aagttaccga tcatggtgcc cagcacttcg
    28681 ttatcgcgcg ggttcaggca gtagagaatg gttttcggca gcaggttttc ttcgttctgc
    28741 ttgctcagca gcttagacaa ctcttccgcc atcggacggt cgttgatgga gtcaaagcct
    28801 acatccggcc ccagcagttt gaactgacgc aggttgttat tacgcagcgc gccaatgtgg
    28861 tactgctgta cccagccgcg acgtgcatat tcggcaccaa ggaacaccag taccgcagtt
    28921 ttgaactgtg ccacttcgtg ctcgctcagg gtttcgccag ccagacggcg cgccagaatg
    28981 ctgtccagtt ccgcttcgtt cgcttcagca aacatcacca cgtccagcgc atggtcagac
    29041 actttacagc cgtgagcggc gaagtgatcc agacgtttgg tcaaggcagt ttgcaggtca
    29101 gcaaagcggc gaatgtcggt atcggaaact tcgcccagct tcgccatgta gtcgttaaag
    29161 gtcgcctgtt cgatattgaa ggctttgtcc ggacgccagc tcggcagcac tttgatggtg
    29221 aaagagccgt ctttggcgat ctctgcgtga tgctccagag aatcgatcgg gtcatcggtg
    29281 gtgccgacca ttttcacgtt catctgctgc atgatgccgc gcgcggagaa gttatcctgc
    29341 gccagcagtt cgttacattc gttccagatt tcatcggcag ttgacggaga aagcaatttg
    29401 ccagtgatac cgaacggacg acgcagttcg aggtgcgtcc agtggtataa cgggttgccg
    29461 atagtgtgcg gaacagtcgc cgcccaggcg tcaaattttt cacggtcaga cgcatcacca
    29521 gtacacagac gctcagccac accgttggta cgcatagcgc gccatttgta gtgatcgccc
    29581 ttcaaccaga tgtcatacag gtttttaaaa cgatagtctt ccgcaatctg ctgcggcggc
    29641 aaatggcaat ggtaatcgaa aatcggctgg tcttttgcgt agtcgtgata cagacggcgg
    29701 gcaaattcgg tatctaacag gaaatcttca gtcataaacg gagtcatttt cgtcttcctc
    29761 tcaacgagtg agctagcttg cttatggtgc gatgctgaca aagttatcac accaatttcc
    29821 agagtccgaa gatattttcg tgagttagat caataaacgt agttaaaaaa attactctca
    29881 aagtggtaaa tctcgctgca ggccgcgcca gtactggcct cgctgtcgtc aggtaatgtc
    29941 cctacaaata ttcgcacatt tgtgatggct ctcacctttt aaggttgtat gacaagttat
    30001 ctttctgccg tcgcaaatca taagtcgacg gaatgcaaat tgccgattca ttcatttgtt
    30061 agatgaatcg ggttaaccgg tacggaagcc gaattagcac gaaactttca tggcaacgtt
    30121 cg
//
LOCUS       DAJTQQ010000042        30012 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000042 DAJTQQ010000000
VERSION     DAJTQQ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.042"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OO309_002083"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OO309_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR5847223.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OO309_002084"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OO309_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR5847224.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="OO309_002085"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="OO309_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR5847225.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="OO309_002086"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="OO309_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR5847226.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="OO309_002087"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="OO309_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR5847227.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="OO309_002088"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="OO309_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR5847228.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="OO309_002089"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="OO309_002089"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR5847229.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="OO309_002090"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="OO309_002090"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR5847230.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO309_002091"
     CDS             complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO309_002091"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR5847231.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO309_002092"
     CDS             complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO309_002092"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR5847232.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO309_002093"
     CDS             complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO309_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR5847233.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO309_002094"
     CDS             complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO309_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR5847234.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO309_002095"
     CDS             complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO309_002095"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR5847235.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO309_002096"
     CDS             complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO309_002096"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR5847236.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18150..18250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO309_002097"
     CDS             complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO309_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR5847237.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18874..19647
                     /gene="nudC"
                     /locus_tag="OO309_002098"
     CDS             18874..19647
                     /gene="nudC"
                     /locus_tag="OO309_002098"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR5847238.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19687..20751
                     /gene="hemE"
                     /locus_tag="OO309_002099"
     CDS             19687..20751
                     /gene="hemE"
                     /locus_tag="OO309_002099"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR5847239.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20908..21579
                     /gene="nfi"
                     /locus_tag="OO309_002100"
     CDS             20908..21579
                     /gene="nfi"
                     /locus_tag="OO309_002100"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR5847240.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21622..22212
                     /locus_tag="OO309_002101"
     CDS             21622..22212
                     /locus_tag="OO309_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR5847241.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22399..22671
                     /gene="hupA"
                     /locus_tag="OO309_002102"
     CDS             22399..22671
                     /gene="hupA"
                     /locus_tag="OO309_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR5847242.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22684..23379
                     /locus_tag="OO309_002103"
     CDS             22684..23379
                     /locus_tag="OO309_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR5847243.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO309_002104"
     CDS             complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO309_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR5847244.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24044..25420
                     /gene="zraS"
                     /locus_tag="OO309_002105"
     CDS             24044..25420
                     /gene="zraS"
                     /locus_tag="OO309_002105"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR5847245.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25417..26742
                     /gene="zraR"
                     /locus_tag="OO309_002106"
     CDS             25417..26742
                     /gene="zraR"
                     /locus_tag="OO309_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR5847246.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO309_002107"
     CDS             complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO309_002107"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR5847247.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO309_002108"
     CDS             complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO309_002108"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR5847248.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7028 a         7823 c         8255 g         6906 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cattgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggcagg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgtgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgt ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccaa gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgattg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaatatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacattgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag gggggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatattctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12601 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    12661 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    12721 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    12781 acggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    12841 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    12901 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    12961 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13021 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13081 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13141 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13201 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13261 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13321 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13381 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13441 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13501 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13561 ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13621 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    13681 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    13741 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    13801 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    13861 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    13921 gcaggcctac ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    13981 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgtggcat
    14041 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14101 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14161 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14221 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14281 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14341 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14401 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14461 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14521 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14581 acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    14641 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    14701 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    14761 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    14821 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    14881 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    14941 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15001 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15061 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15121 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15181 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15241 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15301 cgggctgttg atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15361 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15421 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15481 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15541 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15601 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    15661 cgcgtgccag actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat
    15721 gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    15781 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    15841 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    15901 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc
    15961 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16021 catcggcttc cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc
    16081 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16141 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat
    16201 ttctccgcct ctggcacgga agttctccga catatccgcc attcccactt caatggtttg
    16261 cgcggcggcg taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca
    16321 catggagcaa aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc
    16381 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16441 ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc
    16501 agcgtgggcg gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag
    16561 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16621 cgccgcacca atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg
    16681 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg
    16741 gatcatctgc atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca
    16801 ggcgattttg gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc
    16861 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    16921 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    16981 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17041 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17101 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17161 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa
    17221 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17281 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17341 cgagttgccg atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc
    17401 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17461 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17521 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17581 ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    17641 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    17701 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg
    17761 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    17821 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    17881 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    17941 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18001 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18061 tgcagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca
    18121 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18181 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18241 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18301 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18361 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18421 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18481 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18541 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18601 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    18661 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    18721 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    18781 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    18841 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    18901 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    18961 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19021 cagggggaac ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt
    19081 caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg
    19141 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19201 accgaatggg cgatgctgtg tagccattgc cgtgagcgtt actacccgca aatcgccccc
    19261 tgcattattg ttgccatccg tcgcgatgat tcgctcctcc tcgcccagca tacccgccat
    19321 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19381 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19441 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19501 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19561 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19621 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    19681 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    19741 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    19801 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    19861 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    19921 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    19981 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20041 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa
    20101 gtgccgctga ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc
    20161 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20221 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20281 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
    20341 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20401 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
    20461 atggctgaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
    20521 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20581 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    20641 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    20701 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat
    20761 ccatcaggct attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact
    20821 acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc
    20881 tgttgaacca ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc
    20941 gaactggctt cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc
    21001 gccggagccg atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg
    21061 ctgaaatatc cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg
    21121 ccttacattc caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag
    21181 atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc
    21241 cgtcttggcg tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg
    21301 aaaaaacggc tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca
    21361 ctgatggata aaggcgagca gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg
    21421 ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc
    21481 tgcatgaaag gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa
    21541 cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat
    21601 ttccgtattt gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg
    21661 gaaagctggc agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac
    21721 gccatgttct gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat
    21781 ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag
    21841 aaatttgaag aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca
    21901 atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc
    21961 gaacacgcgg tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact
    22021 caggctggtc gcgaaatggg cgatgaagag cttaaagaaa acccagctgt agagcaagaa
    22081 tgggacattc agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag
    22141 ctgataaaag gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt
    22201 tttcagcaat aagaccagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc
    22261 cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg
    22321 tatgcaggag agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac
    22381 acattgtaag gataacttat gaacaagact caactgattg atgtaattgc agagaaagca
    22441 gaactgtcca aaacccaggc taaagctgct ctggagtcca ctctggctgc aattactgag
    22501 tctctgaaag aaggcgatgc tgtacaactg gttggtttcg gtaccttcaa agtgaaccat
    22561 cgcgctgagc gtactggccg caacccgcag accggtaaag aaatcaaaat tgccgcagct
    22621 aacgtaccgg catttgtttc tggcaaggca ctgaaagacg cagttaagta agattgcgtg
    22681 gcagtgaaca gttttaacga aggggtggtt tcaccccttt tgtctttctg gcgtcgatca
    22741 ttgatgctgg caggtgcgct gtttctcact gcctgtagcc acaactcttc acttcctccc
    22801 tttaccgcca gtggatttgc tgaagaccag ggcgcggtac gcatctggcg aaaagacagc
    22861 ggcgataatg tgcatctgct tgccgtgttt agcccgtggc gcagtggcga taccacgacg
    22921 cgagagtatc gctggcaggg cgataacctc acgctcatca atatcaatgt ttacagcaaa
    22981 ccgccggtga acattcgcgc gcgttttgac gatcgcggcg atctgagctt tatgcaacgt
    23041 gaatccgatg gggaaaagca gcagctttct aacgaccaaa tcgatttata ccgttatcgt
    23101 gctgatcaga tccgccagat tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag
    23161 gggcgctggc atgcgatgga acagaccgtg accacctgcg aagggcaaac cattaaacct
    23221 gatttagatt cgcaggcgat agcgcatatc gagcgccgcc agagccgctc ttctgttgat
    23281 gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat tactgttagt ggcaaactct
    23341 gatttctgtc gctggcaacc caacgagaaa acgttctgat ttaccagtgg cccataccca
    23401 tatgaccgcc accaccgcag ccgccgtagc ccatgcccat tccggctccg cgcggaatac
    23461 ccgcttcagc catcgcgata tctcgtttca cccgtaactc atctaacgac tgacgcaaat
    23521 tctccatctc tttggcgacc gcgttaattt tgctgctatc cggtgggttc gcggctaaca
    23581 gggcattgta ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt
    23641 aaaagtcatt atggattttc tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat
    23701 tttgctgcca cataccgtgt ccgccgtgag caaatgcaga tgtcgatccc atcgccattg
    23761 ctgaaagcgc catcattacc agggcaattt tcgtgttccg tttcatggtt aatcctccag
    23821 tggttgtctt acttcgggta ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg
    23881 gtaatctggc atgataaacg agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt
    23941 tactcattga aacttaagcc tttgtgttac agcgcagggt aagcgctgat aaaagatggc
    24001 atgatttctg ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg
    24061 ttctaaagac tccttagcta aatggttaag cgcgatcctc cccgtggtca ttgttgggct
    24121 ggtagggttg tttgcggtga cggtgattcg cgattatggg cgcgagactg ccgccgccag
    24181 acaaacgctg ctggaaaaag gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt
    24241 cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc ttactggaag aaatggccgg
    24301 gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag
    24361 caactccggc atggtgggaa aacagcttta ttcccctcag gaaatgcagc agttacatcc
    24421 gggagatgaa gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg ttctggaaat
    24481 ttatcgccag tttcaaccga tgtttgctgc tggaatgtac cggatgcgcc atatgcagca
    24541 gtatgccgcg acaccacaag caattttcat cgctttcgac gccagtaata ttgtgagtgc
    24601 cgaagatcgt gagcagagaa acaccctgat tattctcttc gccctggcga cggtcttgct
    24661 ggcaagcgta ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc agcttctaca
    24721 agatgaaatg aagcgcaaag agaagctggt ggcgctgggg catcttgcgg caggcgttgc
    24781 ccacgaaatc cgtaacccac tttcctcgat taaaggactg gcgaaatact ttgccgagcg
    24841 cgcgcctgca gggggagaag cgcatcaact ggcgcaggtg atggcgaaag aggccgaccg
    24901 tttaaaccgc gtggtaagcg agttgctgga actggttaag ccaacgcatc tggctttgca
    24961 ggcggtggat ctcaacacgc tgattaacca ctcattacag ctggtaagcc aggatgcaaa
    25021 cagccgggag atccagttac gctttaccgc caacgacaca ttaccggaaa ttcaggccga
    25081 tccggacagg ctgactcagg tcctgttgaa tctctatctc aatgctattc aggcgattgg
    25141 tcagcatggc gtgattagcg tgacggccag cgaaagcggc gcgggcgtga aaatcagcgt
    25201 taccgacagc ggtaagggaa ttgcggcaga tcagcttgaa gccatcttca ctccgtactt
    25261 caccaccaaa gccgaaggca ccggattggg gctggcggtc gtgcataata ttgttgaaca
    25321 acacggtggt acaattcagg tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg
    25381 gcttccggtc aatattacgc gtaaggaccc acaaggatga cgcacgataa tatcgatatt
    25441 ctggtggtgg atgatgacat tagccactgc actattttgc aggctttact gcgcggctgg
    25501 ggctataacg tcgcgctggc gaacagcggg cgacaggcgc tggagcaggt gcgggaacag
    25561 gtttttgatc ttgtgctttg cgatgtgcga atggcggaga tggacggcat cgccacgctg
    25621 aaagagatca aagcgttaaa cccggcaatt ccggtgctga ttatgactgc gtactccagc
    25681 gtcgagacgg cggtagaggc actgaaaact ggggcgcagg attatctcat caagccgctg
    25741 gatttcgata acctacaggc gacgctggaa aaggcgctcg cgcatacgca cagtattgat
    25801 gctgaaacac ctgcggtgac tgccagccag ttcggtatgg tcggtaaaag cccggcgatg
    25861 caacacctgc tcagtgaaat cgccctcgtc gcgccatcgg aagccacggt gctgatccac
    25921 ggcgattccg gcaccggtaa agagctggtc gccagggcga ttcacgccag tagcgcacgt
    25981 agcgaaaaac cgctggtaac gctcaactgt gcggcactca acgaatcctt gctggaatct
    26041 gaattgttcg gtcacgaaaa aggggcgttt actggagccg ataaacgccg ggaagggcgc
    26101 tttgttgagg cggacggcgg cacgctgttt ctcgatgaaa ttggcgatat ctcgccgatg
    26161 atgcaggtgc gtctgctgcg tgcgattcag gagcgcgaag ttcagcgtgt cggtagcaac
    26221 cagactatct cggttgatgt ccggctgatt gcggcgaccc atcgcgatct tgccgcagag
    26281 gtgaatgccg ggcgttttcg ccaggatctc tactatcgcc tgaatgtggt ggcgattgaa
    26341 gtaccatcgc tgcggcaacg gcgggaagat atccctctgc tggctggcca ttttcttcag
    26401 cgctttgccg agcgtaatcg caaggcggta aaaggcttta cgccacaggc gatggatttg
    26461 ctgatccact acgactggcc gggaaatatt cgtgaactgg aaaacgcggt ggaacgggcg
    26521 gtggtgctgc tgaccgggga atatatttcc gaacgcgagc tgccgctggc gattgccagt
    26581 acgccgatcc cgctggggca aagtcaggat attcagccgc tggtggaagt ggaaaaagag
    26641 gtgattctgg cggcgctgga gaaaacgggc ggcaacaaaa ccgaagccgc ccgtcagtta
    26701 gggatcacgc gcaaaacgct attggcaaaa ctgtcgcgtt agttctgctc gcgttcgatg
    26761 gcgcgccaac cgatatcttt ccggcagaag caatcatccc agtggatatc ggtcattaag
    26821 gcgtaggcgc gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac gcacagtacg
    26881 cgcccgccgc tggttaccac ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact
    26941 ttgccgtcag ccacttcttc cagcggcagg ccgtggatca catcgccggt gcggtaatcg
    27001 cccggatatc cacccgcagc catcaccacg ctgagagaag cacgttcatc ccactctgac
    27061 gttttctcgt ccagtttgcc ttcacaggcc gccaggcaga gctcaaccag atcggacttc
    27121 atgcgcagca taatcggctg ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc
    27181 ttcggattgc cctgtttgtc gatcatcagg cccgcataga gaaaaccggt gtaggtgttg
    27241 ccttccgccg ccatgccttt cacggttggc cagatgatac gttccatggt gcgctgatga
    27301 acttcatcgg ttactaccgg cgcgggggag taagcgccca tcccgccggt gtttggtccg
    27361 ttatctttat cgcctacgcg tttgtgatcc tggctggtag ccatcggcag cacatgctcg
    27421 ccgtccacca tcacgataaa gctcgcttct tcgccatcga ggaactcttc gataacgatg
    27481 cgatgacccg cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc cgcttccgct
    27541 tcttccagcg tcatcgccac gataacgcct ttcccggcag ccagaccgtc cgctttaatg
    27601 acgattggcg cgcctttctc acgcagatac accagcgcag gttctacctc ggtgaagttc
    27661 tggtattccg ccgtagggat cttatggcgg gccaggaaat ctttggtaaa cgcttttgag
    27721 ccttccagtt gggccgcacc tgcggttggg ccgaagattt tcagcccggc ggcgcggaag
    27781 gtatcgacca cgcctttcac cagcggcgct tccgggccga cgatggtcag atcaatcttt
    27841 tcgttttgtg cgaaatccaa cagcgccggg atatcggtca cgccgatagc aacgttttgc
    27901 agcgcgggtt ccagcgcagt gcctgcatta cccggagcag caaaaacagt ctcaaccagc
    27961 ggcgactggg ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat
    28021 actttcatct attgctccat taatggcgga agtggcgcat gtcggtgaag agcatcgcaa
    28081 taccgtgctc gtcggcggcg gcaatcactt cgtcatcacg gatggaaccg ccaggctgga
    28141 ttacgcaggt cacgcccgca gcggcggcgg catcaatacc gtcgcggaac gggaagaacg
    28201 cgtcagaagc catcgaggaa cctttcactt ccaggccttc atcggcagct ttaataccgg
    28261 cgattttcgc ggagtacacg cggctcatct ggcccgcgcc aataccgatg gtcatattgt
    28321 ttttggcata gacgatagca ttggatttca cgaatttcgc cactttccag cagaacagcg
    28381 catcacgcag ttcctgttcg gtcggctgac gttgggtgac gacgcgcagt tcttccgcac
    28441 cgaccatccc caggtcacga tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa
    28501 gacccggaac acgctcgccc cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg
    28561 cggtgatttt cagggcttct tcgctggcgg aaggcgcaat aatcacttcg acaaactgac
    28621 gagaaatgat ggcctgtgcg gtttccgcat ccagctcgcg gttaaaggca atgatgccgc
    28681 cgaatgcgga ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga atggaattgc
    28741 cgatagctac accgcaaggg ttggcgtgct tcacaatcac acatgccggc tcggcgaact
    28801 ctttcacgca ctccagcgcc gcatcggtat cggcgatgtt gttataagag agggctttac
    28861 cctgaacctg ggttgcggta gcaacggagg cttctttcac attctcttct atatagaagg
    28921 cagcctgctg gtggctgttc tcgccgtaac gcatatcctg cttcttaatg aagttcaggt
    28981 tcagcgtgcg tgggaagcga ccggcggctt ctttgctttc accgtggtac gccggaacca
    29041 tgctgccgaa gtagttggca atcatgctgt cgtaggcggc agtgtgttcg aaggctttga
    29101 tggcgaggtc gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc atctctttaa
    29161 taatggcgtc gtagtcgctg ctcttcacca cgattgcgac atctttatgg ttcttagcgg
    29221 cggagcgcac catggtcggg ccgccgatat cgatgttctc aaccgcatct tccagcgagc
    29281 agccttcacg agcaacagtc tgggcgaacg gatacaggtt aacaaccacc atatcgatag
    29341 gctggatctg atgttcttcc ataatggcat cgtcctggcc gcgacggccc agaatgccac
    29401 catgtacttt cggatgcagg gtcttcacgc gtccatccat catctccggg aaaccggtgt
    29461 aatcggaaac ttcggttacc ggcagacctt tttctgctaa cagacgggca gtgccccctg
    29521 tagacagcag ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt
    29581 tgtcagaaac actgagcaga gcgcggcgga ctggacgacg ttgttgcatg gtaaatcccc
    29641 tgggtttgac tattacagag agcgttagct gaatttttcg cgaaaaactc agctaacgcc
    29701 cctaacgggg catccttatt tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg
    29761 cgaatttttc tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac
    29821 agaaattaca ttgatgattg tggataactc tgtgcgtaaa aaggtataaa gcggggtttt
    29881 gctggggaat gcagcagtca gtcatttttc tgcaattttt ctattgcggc ctgcggagaa
    29941 ctccctataa tgcgccttca tcgacacggc ggatgtgaat cacttcacac aaacagccgg
    30001 ttcggttgaa ga
//
LOCUS       DAJTQQ010000043        29623 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000043 DAJTQQ010000000
VERSION     DAJTQQ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29623
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.043"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..676
                     /locus_tag="OO309_002109"
     CDS             <1..676
                     /locus_tag="OO309_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5847249.1"
                     /translation="ARLYDAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEI
                     VARYPQCQRVDSKLIETWFNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEI
                     YESVINRIRTEFPHADDITMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNP
                     LNKIICEETIRLGGSMVHHHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGT
                     IYPIEK"
     gene            698..2107
                     /locus_tag="OO309_002110"
     CDS             698..2107
                     /locus_tag="OO309_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5847250.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2085..2864
                     /locus_tag="OO309_002111"
     CDS             2085..2864
                     /locus_tag="OO309_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR5847251.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            2861..3721
                     /locus_tag="OO309_002112"
     CDS             2861..3721
                     /locus_tag="OO309_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR5847252.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(3869..4444)
                     /locus_tag="OO309_002113"
                     /pseudo
     CDS             complement(3869..4444)
                     /locus_tag="OO309_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4461..4721)
                     /locus_tag="OO309_002114"
     CDS             complement(4461..4721)
                     /locus_tag="OO309_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5847253.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4712..5983)
                     /locus_tag="OO309_002115"
     CDS             complement(4712..5983)
                     /locus_tag="OO309_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5847254.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6061..6423)
                     /gene="queD"
                     /locus_tag="OO309_002116"
     CDS             complement(6061..6423)
                     /gene="queD"
                     /locus_tag="OO309_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR5847255.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6742..8541
                     /gene="cysJ"
                     /locus_tag="OO309_002117"
     CDS             6742..8541
                     /gene="cysJ"
                     /locus_tag="OO309_002117"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR5847256.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8541..10253
                     /gene="cysI"
                     /locus_tag="OO309_002118"
     CDS             8541..10253
                     /gene="cysI"
                     /locus_tag="OO309_002118"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR5847257.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10327..11061
                     /gene="cysH"
                     /locus_tag="OO309_002119"
     CDS             10327..11061
                     /gene="cysH"
                     /locus_tag="OO309_002119"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR5847258.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11325..11477
                     /locus_tag="OO309_002120"
     CDS             11325..11477
                     /locus_tag="OO309_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR5847259.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11671..12762
                     /locus_tag="OO309_002121"
                     /pseudo
     CDS             11671..12762
                     /locus_tag="OO309_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(12908..13945)
                     /gene="iap"
                     /locus_tag="OO309_002122"
     CDS             complement(12908..13945)
                     /gene="iap"
                     /locus_tag="OO309_002122"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR5847260.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14197..15105
                     /gene="cysD"
                     /locus_tag="OO309_002123"
     CDS             14197..15105
                     /gene="cysD"
                     /locus_tag="OO309_002123"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR5847261.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15107..16534
                     /gene="cysN"
                     /locus_tag="OO309_002124"
     CDS             15107..16534
                     /gene="cysN"
                     /locus_tag="OO309_002124"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR5847262.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16534..17139
                     /gene="cysC"
                     /locus_tag="OO309_002125"
     CDS             16534..17139
                     /gene="cysC"
                     /locus_tag="OO309_002125"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR5847263.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17189..17512
                     /locus_tag="OO309_002126"
     CDS             17189..17512
                     /locus_tag="OO309_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR5847264.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17706..18017
                     /gene="ftsB"
                     /locus_tag="OO309_002127"
     CDS             17706..18017
                     /gene="ftsB"
                     /locus_tag="OO309_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR5847265.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18036..18746
                     /gene="ispD"
                     /locus_tag="OO309_002128"
     CDS             18036..18746
                     /gene="ispD"
                     /locus_tag="OO309_002128"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR5847266.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18746..19225
                     /gene="ispF"
                     /locus_tag="OO309_002129"
     CDS             18746..19225
                     /gene="ispF"
                     /locus_tag="OO309_002129"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR5847267.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19222..20271
                     /gene="truD"
                     /locus_tag="OO309_002130"
     CDS             19222..20271
                     /gene="truD"
                     /locus_tag="OO309_002130"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR5847268.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20252..21013
                     /gene="surE"
                     /locus_tag="OO309_002131"
     CDS             20252..21013
                     /gene="surE"
                     /locus_tag="OO309_002131"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR5847269.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21007..21633
                     /gene="pcm"
                     /locus_tag="OO309_002132"
     CDS             21007..21633
                     /gene="pcm"
                     /locus_tag="OO309_002132"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR5847270.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21773..22912
                     /gene="nlpD"
                     /locus_tag="OO309_002133"
     CDS             21773..22912
                     /gene="nlpD"
                     /locus_tag="OO309_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR5847271.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            22975..23967
                     /gene="rpoS"
                     /locus_tag="OO309_002134"
     CDS             22975..23967
                     /gene="rpoS"
                     /locus_tag="OO309_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR5847272.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24087..24494)
                     /locus_tag="OO309_002135"
     CDS             complement(24087..24494)
                     /locus_tag="OO309_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR5847273.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24641..25234
                     /locus_tag="OO309_002136"
     CDS             24641..25234
                     /locus_tag="OO309_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR5847274.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25234..26181
                     /locus_tag="OO309_002137"
                     /pseudo
     CDS             25234..26181
                     /locus_tag="OO309_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26454..27017)
                     /locus_tag="OO309_002138"
     CDS             complement(26454..27017)
                     /locus_tag="OO309_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5847275.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27182..27433
                     /locus_tag="OO309_002139"
     CDS             27182..27433
                     /locus_tag="OO309_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5847276.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27435..29531
                     /locus_tag="OO309_002140"
     CDS             27435..29531
                     /locus_tag="OO309_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5847277.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         7241 a         7340 c         8027 g         7015 t
ORIGIN      
        1 cgctcgtttg tatgacgctg aagatggcac ccaacacttc acccattttg ccgacggaaa
       61 atgtgtgctg atctttatgg ctgaaggtaa ccctcgcatt gcgaaggcga cgggcgaagg
      121 gattgcggaa atcgttgccc gctacccgca gtgccagcgc gtggacagca aactgattga
      181 aacctggttt aacaacctga actggggacc ggataaagtg gctgccgaac gtgtgcagat
      241 cctcaaaacc ggcaacatgg gctttaccac cgaagtgtcc ggctgctgga gctgcatcca
      301 cgaaatctac gaaagcgtta ttaaccgtat tcgtactgag ttcccgcacg ccgacgacat
      361 caccatgctg ggcggtcact cctctcatag ctatcagaac ggcaccaaca tgtacttcgt
      421 ctatgactac aacgttgttg actgtaagcc ggaagaggaa atcgacaagt accacaatcc
      481 gctcaacaaa atcatctgcg aagaaaccat tcgcctcggc ggttcgatgg tgcaccacca
      541 tggcattggt aaacatcgcg ttcactggag caagctggaa cacggcagcg cgtgggcgtt
      601 gctggaaggg ctgaaaaagc agttcgatcc taacggcatt atgaatacgg gtactatcta
      661 tccgattgaa aaataatgta tcaggcagcg ttccgcgatg acggggcgtt gccttttcgg
      721 cttctcaggc gagaagccgt tcttattacc ggacaatgaa ggggtaaaga tgaacacttc
      781 accggtgcga atggatgatt taccgcttaa ccgttttcac tgtcgaattg ctacgctcac
      841 tttcggcgca cacctgaccg acggttatgt tctaggcgtc attggttacg ccattattca
      901 acttacgccc gccatgcaac tgacgccgtt tatggcggga atgatcggcg gctcggcgct
      961 ccttggtttg tttcttggca gcctggttct tgggtggatc tccgaccata ttggtcggca
     1021 aaaaatcttc accttcagct ttttgctgat tacgctcgct tcgttcttgc aattttttgc
     1081 caccacgcca gagcatctta ttgggctgcg cattttgatc ggcattggtc tgggaggcga
     1141 ttactcagta ggtcacacct tgctggctga attttccccg cgccgccatc gcggtatttt
     1201 gctgggcgca ttcagcgtgg tgtggaccgt aggctatgtg ttggcaagta ttgccggaca
     1261 tcactttatt tccgaaaacc cggaggcctg gcgctggttg ctggcatcgg cagctctgcc
     1321 cgcgttgttg attacgttat tacgctgggg aacgccagaa tcgccacgtt ggctactgtg
     1381 ccaggggcgt tttgcagaag ctcacgctat cgtgcatcgc tattttggtc cccatgtttt
     1441 actgggcgat gaagtggtaa cggcgaccca taaacacatc aaaaccttgt tctcttcgcg
     1501 ttactggcgg cgcacggcgt ttaacagcgt cttctttgtc tgcctcgtaa tcccatggtt
     1561 tgtgatttat acctggctgc caactatcgc ccagactatt ggtctggaag atgcgctgac
     1621 tgccagcctg atgcttaatg cgttgttaat tgtgggcgcg ctgctgggat tagttctgac
     1681 gcacctgctg gcacatcgca aatttttgct gggaagtttt ttgctgctgg cggcaacgct
     1741 ggtagtaatg gcctgtttgc cttccggcag ttcattaacg ctgctgcttt ttgttctctt
     1801 cagcaccacc atttcggcag tcagtaatct ggtgggcatt ttgcctgcgg aaagttttcc
     1861 tactgacatt cgctcgctgg gagtcggttt tgccaccgcc atgagtcgac ttggcgcggc
     1921 ggtaagtact ggcctgctgc cgtgggtgct ggcgcagtgg ggaatgcaag tcaccttatt
     1981 gctcctagcg acagtgttgt tggttggttt tgttgtgacc tggctatggg caccagaaac
     2041 taaagccctc ccgctggtgg cggcgggaaa tgtaggaggt gcgaatgaac attctgttag
     2101 cgtttaaagc cgaaccggat gccggaatgc tggcggaaaa agagtggcag gcggcggctc
     2161 agggtaatag cggaccggat gtttcgctac tgcgaagttt actcggtgct gatgaacagg
     2221 ccgctgccgc gctgctgtta gcgcagagaa aaaacggtac gccgatgtct ttaaccgcgt
     2281 tgagcatggg ggatgaacgg gcgttgcact ggctgcgcta tctcatggct cttgggtttg
     2341 aggaagctgt attgctggag acggcggcag atctgcgctt tgcgccggaa tttgtcgctc
     2401 gccatattgc cgaatggcag cgtcagaatc cgctggatct gataattacc ggcagccaaa
     2461 gcagcgaagg gcagaatggg caaacgccat ttttgctggc ggaaatgctg ggctggccct
     2521 gcttcaccca ggtggagcgt ttcactctcg acgcgctgtt tatcaccctc gaacagcgta
     2581 ctgaacatgg gttgcgctgt tgccgggttc gcttgcctgc ggtgattgcc gtgcgtcagt
     2641 gcggtgaagt ggccttgcca gtaccgggaa tgcgccagcg aatggctgcg gggaaagcag
     2701 aaattatccg taaaactgtt gctgccgaaa tgccagcgat gcagtgtctg caactggcaa
     2761 gagcagagca acgacgcgga gcgacgctga ttgatgggca aacggtagca gaaaaagctc
     2821 aaaagctatg gcaggactat ttacgccaaa ggatgcagcc atgaatatcg caattgtgac
     2881 aataaatcag gagaatgccg caatagcgag ctggctggcg gcgcaggatt tcagcggttg
     2941 tacgctggcg cactggcaaa tagaacctca gcccgtagtg gcggaacagg tgctggatgc
     3001 gatggtggaa cagtggcagc gaacgcctgc ggacgttgtt ctctttccgc cgggaacgtt
     3061 tggcgatgaa ctgtcgaccc ggctggcctg gcgtttacat ggtgcaagca tctgtcaggt
     3121 gacttcgtta gatattccaa ccgtaagcat gcgcaaatcc cactggggaa atgcgttaac
     3181 ggcgacgctg caaacagaaa aacgcccgtt atgcctttcc ctggcgcgtc aggcgggagc
     3241 agcgaaaaac gccacgttgc cctctggaat gcagcaactg aacattgtcc caggtgcgct
     3301 tcccgactgg ctggttagca cagaggatct gaaaaatgtt agccgcgacc cattagctga
     3361 ggccagacgg gtgctggtgg tgggacaggg cggagaggca gataaccagg aaatcgttat
     3421 gctggcagaa aagctgggag ccgaagtggg ctacagtcgg gcgcgagtga tgaacggtgg
     3481 cgttgatgct gaaaaagtga ttggtatttc cggacatctg ctggcacctg aggtgtgcat
     3541 tgttgttggc gcttccggcg cagcagcgtt aatggcgggc gtacgcaata gcaagtttat
     3601 ggtggcgatt aatcatgacg ccagcgcagc ggtattttca caggcagata ttggcgtggt
     3661 tgatgactgg aaagtggtgc tggaggcact ggtgacaaac attcacgcag aataccaata
     3721 acgtgctgcc ggatgatcct gcctacggtt ctggactggg gacctgtagg cctgataaga
     3781 cgcgttaagc gtcgcatcag gcgctgaatg tcggatgtgg cgtgcggcca acaaatcaaa
     3841 acaaattcaa ttggttacga ttatcccgtc aaagcaattt tttcgctaac gtccagactc
     3901 cggtattcgt ggtggaaagc gccacgacac ccgcgttaat cgcattacgc gcatcttctt
     3961 catcgcatac cagcccgcca gcaatcagcg gttggcggat tttttccgtc acccagccca
     4021 gcactttggg catgcagcct ggcaggatct caatacaatc cggattcgat tgcgcaactt
     4081 acttatcaat gttgtgaaac gaaatcgaat caacaataaa caggtgatga atacagaaaa
     4141 aaacttccgc tcttgccgct tttagcatcg atgctttggt gctgataatt ccatccgcct
     4201 cggtcaccag tttcaaaaac tgaatcacca cttctttatt tgacgcgcct tccagcaaat
     4261 caacatgaat aaaagcatat ttcccggcgt tcttaatctt tttaacgata ttactgatgg
     4321 tgcagatatt gccgtacaac acggaaataa actggcattc agaatcgatg gccagttgca
     4381 ggctggcatt atctttaacg gcagcaatca ccggattctg gcggagcagg tgtaagaggg
     4441 gcataaatta tccttattat ttatccaaag cggtaggtga tgccgaatcc ggaaggcgga
     4501 tagcgccact gttgtagtgt ggattcgtcg cacagcaaac ggcaggtgcc acactccagg
     4561 cagccgcgat agtcaatacg taagtcacct tcctgtgtga gagaaaaaag acctaccgga
     4621 caggcgttaa tcaaccgttg taccatctga cgctcaacgg agtcagccgg aacaatgtgc
     4681 ggcgcatcag caacgcgcca gagattacgg gctacagaca tcgcagactc ctgataacat
     4741 cgcccgccag atgccacagg ccatgacgac gtaaatgatg ccagaataac tggcgcagcg
     4801 gtggaacagg tttatcaccc tgatcccata aatcgcggga aatatcctgc attaacgcag
     4861 gccacgcccg atacctgcca gggcgttgca aaagcgccgg aacatgctga taacgttgta
     4921 gaacatccca tagcaggctg cgctctacgt tgtgatgata aagcgcaaac agattttgcg
     4981 gctcgcggtg ctggcaggcg cttattagcg tttgtgccgc cgcctgtgcg ccagtcagcg
     5041 ccatatccat gccgcgcacg gaaattccgg tattgacgca actgcgcaac gcatcgccca
     5101 ccagcagcca gccgttaccg gcgtattgca ccggcatact gtgcaagcca ccttctggca
     5161 ccagatgcgc accatactcc agtgattccg tgtttttgat aagcgtgcgc actgccggat
     5221 gcgctttaaa gcgagtcagc agctcgcttg ccggaacacg actttgcgta agggaagaga
     5281 gcgggcaaac aatccctaac gagagcgttt gttgattagt ataaagaaat gcgccgccgg
     5341 gtaagtcatc acagattccg ccgctgaaca gcaacgctgc gccttcgtta ttctccagat
     5401 gaaaacgttc ttcaatagcg gatgtttcca gcgacagcac ttctttgatc cccaacgcca
     5461 tcgcttcgcc agcaggacga gtcactaacc cgtgacgttc agccaggacg ctgttggcac
     5521 cttctgccag caccacataa cgggcgcgga gaatatcgtc accacaaata acgccacaga
     5581 ctctgccgtt ttcttcatac agtgcatcca ccgtcgctcc ggggatgcat tcgacacctt
     5641 ctttttcggc ttcggcaacc agccacggat cgaaccgtgc acgtaataca ttccaggatt
     5701 caccgccggg ctgtaagctg gaaaacgtcg ttgcgccatc cggcgttaac agggaaaggc
     5761 tttcgtgagt gatgcgtcgt tcaagaggcg cggtcagatg aaattgcggg aggagttccg
     5821 cgagtgcatg ggtatataac cgcctgccgg aaagattttt gctgccgggg atttcagcgc
     5881 gttcgagtaa caaaacggat aaatccgctc gcgcgcagcg taacgcgcaa gcggtgcctg
     5941 caataccggc accaataata ataatgtcgc agtcgtcttc catgtcatgc cccagatatc
     6001 cagaacacaa caccctaaca tagcgttact aaagggaaat tgaccgccga cacactctta
     6061 tcattcgccg cgatagatac accctgcggt gcaggtttct tttaccatca ccgcacttaa
     6121 cagcggcaca acgggtttaa cctgatccca aatccatttt gctaaaactt cgctggttgg
     6181 attttccaga cctggaatat cattgagata atggtgatcg aggcgctcat aagttggctt
     6241 aaacgccgct ttcagttcag cgaaatcgat aatccagccc gtatgcggat cgacttcccc
     6301 ggtaatttcc agtcgcacca taaaggaatg cccgtgcagg cgaccacatt tatgcccttc
     6361 cgggacatgt ggtaagcggt gagcggcttc gaaggtgaaa tctttaaata acgtggtgga
     6421 catcataatt tctctacagg acgataaaac cgccgtagag taccggaaag gctgtttttt
     6481 cgcattatct aaaacgactt tgttgcgcaa aatcgctgat ttatcttaat gattggctaa
     6541 attcatttgt ttttcattag gttggttaat ctattttgtt gttaaagact attgctaaaa
     6601 caggttagtc gatttggtta ttagttatcg ctatcccgtc tttaatccac accgttcacc
     6661 ccgttaacct taccttctct tctgttttat gggcgctgac agggcgcaga aacagctttg
     6721 cttactggaa cataacgacg catgacgaca caggtcccac cttccgcgtt gcttccgttg
     6781 aacccggagc aactggcacg ccttcaggcg gccacgaccg atttaacgcc tacccagctt
     6841 gcctgggttt ctggctattt ctggggcgtg ctcaaccagc agcctgttgc gcttgcagtg
     6901 acgccagcgc ccgccgcaga aatgccgggt ataacgatta tctctgcttc gcaaaccggc
     6961 aatgcgcgcc gggttgctga agcattacgc gatgatttat tagcagcaaa actgaacgtt
     7021 aagctggtga acgcgggcga ctataaattc aaacaaatcg ccagcgaaaa actgctcatc
     7081 gtagtgacgt caacgcaagg ggaaggggaa ccgccggaag aagccgtcgc gctgcataag
     7141 ttcctgttct ccaaaaaagc gccaaagctg gaaaacaccg cgtttgccgt gtttagcctc
     7201 ggcgatagct cttatgaatt tttctgccag tccgggaaag attttgacag caagctggcg
     7261 gaactggggg gtgaacgcct gctcgaccgt gtcgattctg atgttgaata ccaggctgct
     7321 gccagcgagt ggcgcgcccg cgtggttgat gcgcttaaat cgcgtgcgcc tgtcgcggca
     7381 ccttcgcaat ccgtcgctac tggcgcggta aatgaaatcc acaccagccc gtacagcaaa
     7441 gacgcgccgc tggtggctag cctctctgtt aaccagaaaa ttactgggcg taactctgaa
     7501 aaagacgttc gccatatcga aattgactta ggcgactcgg gcctgcgtta ccagccgggt
     7561 gacgcgctgg gcgtctggta tcagaacgat ccggcactgg tgaaagaact tgtcgaactg
     7621 ctgtggctga caggcgatga acctgtcact gtcgagggca aaacgttgcc tctgaacgaa
     7681 gcgctacagt ggcacttcga actgaccgtc aacaccgcca acattgttga gaattacgcc
     7741 acgcttaccc gcagtgaaac actgctgccg ctggtgggcg ataaagcgaa gttacagcat
     7801 tacgccgcga cgacgctgat tgttgacatg gtgcgtttct ccccggcaca gcttgatgcc
     7861 gaagcgctaa ttaatctgct gcgcccgctg accccgcgtc tctattccat tgcctcctcg
     7921 caggcggaag tcgagaacga agtacacgtc accgttggtg tggtgcgtta cgacgtggaa
     7981 ggccgcgccc gtgccggtgg tgcctccagc ttcctcgctg accgcgtgga agaagagggc
     8041 gaagtccgcg tatttatcga acataacgat aacttccgcc tgcccgctaa cctggaaacc
     8101 ccggtgatta tgattggccc aggcaccggt attgcgccgt tccgcgcctt tatgcagcaa
     8161 cgcgccgctg acgaagcgcc aggtaaaaac tggctgttct ttggcaatcc gcattttacg
     8221 gaagacttcc tgtatcaggt ggagtggcag cgctacgtca aagagggtgt gctgacgcgg
     8281 atcgatctcg cctggtcgcg cgaccaaaaa gaaaaagttt acgtacaaga taaactgcgc
     8341 gaacagggcg cggagctgtg gcgctggatc aatgacggtg cccacattta tgtctgcggc
     8401 gacgctaatc gcatggcgaa agacgttgag caggcacttc tggaagtgat tgccgaattt
     8461 ggtggcatgg acaccgaagc ggcggatgaa tttttaagtg agctgcgcgt agagcgccgt
     8521 tatcagcgag atgtctacta atgagcgaaa aacatccagg gcctttagtg gtcgaaggaa
     8581 aactgacaga cgccgagcgc atgaagcttg aaagcaacta cctgcgcggc accattgcgg
     8641 aagatttaaa cgacggtctg accggcggct ttaagggcga taacttcctg ctgatccgct
     8701 tccacggcat gtatcagcag gatgaccgcg acatccgcgc cgaacgtgct gaacagaagc
     8761 tggagccgcg ccacgcgatg ctgttgcgct gccgtctgcc gggtggagtt atcaccacca
     8821 aacagtggca ggcgatcgat aaatttgccg gtgaaaacac catctatggc agcattcgcc
     8881 tgaccaaccg ccagacgttt cagttccacg gcattctgaa aaagaacgtc aaaccggtgc
     8941 accagatgct gcactcggtc ggtcttgatg cgctggcgac tgctaacgac atgaaccgta
     9001 acgtactctg cacctcgaac ccttacgagt cgcagctgca cgcagaagcg tacgagtggg
     9061 cgaagaagat ttctgaacat ctgctgccgc gtacccgcgc gtatgcggag atctggctcg
     9121 atcaggaaaa agtcgccacc accgatgaag aaccgatcct tggtcagact tacctgccgc
     9181 gtaagttcaa aaccacggta gtgatcccgc cgcagaacga tattgatctg cacgccaacg
     9241 acatgaactt cgtggcgatc gcggaaaacg gcaagctggt gggcttcaac ctgctggtgg
     9301 gcggtgggct ttctatcgaa cacggtaaca agaaaaccta cgcccgcacg gcgagtgagt
     9361 ttggctatct gccgctagag catacgctag cggtggcgga agccgtcgtg acaactcagc
     9421 gtgactgggg taaccgaacc gatcgtaaaa atgccaaaac caaatacaca ctggagcgcg
     9481 tgggggttga gacgtttaaa gcggaagtgg agcgtcgcgc ggggatcaaa tttgaaccga
     9541 tccgcccgta tgaatttaca ggacgcggcg atcgtattgg ctgggttaag ggcattgatg
     9601 ataactggca cctgacgctg tttatcgaaa atggtcgcat ccttgattat ccggggcgtc
     9661 cgctgaaaac tggcctgctg gagatcgcga agatccacaa aggtgatttc cgcattacgg
     9721 cgaaccagaa tctgatcatc gccggtgtgc cggaaagcga gaaagcgaag atcgagaaaa
     9781 ttgccaaaga gagcgggtta atgaatgccg tcacgccgca gcgtgaaaac tcgatggcct
     9841 gcgtgtcatt cccgacttgc ccgctggcga tggcggaagc ggagcgtttc ctgccgtctt
     9901 ttatcgacaa catcgataat ttaatggtga aacatggtgt cagcgatgag catatcgtga
     9961 tgcgtgtaac aggctgcccg aacggttgtg gtcgcgcgat gctggcggaa gtgggcctgg
    10021 tgggtaaagc accgggtcgc tacaacctgc atcttggcgg caaccgcatt gggacacgta
    10081 tcccacggat gtataaagaa aacatcaccg agccggaaat cctggcgtcg cttgatgaac
    10141 tgatagggcg ctgggcgaaa gagcgcgaag cgggtgaagg cttcggcgac tttacggtgc
    10201 gtgcgggcat cattcgcccg gtgctcgatc cggcgcgcga tttgtgggat taaccatcag
    10261 cccggtcttg taggcctgat aagacgcgtg agcgtcgcat caggcaaggc aaacagtgag
    10321 gaatctatgt ccaaactcga tctaaacgcc ctgaacgaac tgccgaaggt agatcgcatt
    10381 ctggcgctgg cagaaactaa cgccgaactg gaaaaactgg acgctgaagg ccgcgttgcc
    10441 tgggcgctgg ataatctgcc cggtgaatat gtactttctt ccagctttgg cattcaggcg
    10501 gcggtgagcc tgcatctggt gaatcagatt cacccggata ttccggtgat cctcaccgac
    10561 accggctatc tgttcccgga aacctaccgc tttattgacg agttaacgga caaactcaag
    10621 ctcaacctga aagtgtaccg cgctaccgaa agcgccgcct ggcaggaagc acgctacggc
    10681 aaactgtggg aacagggcgt tgaaggcatt gaaaagtaca atgacatcaa caaagtcgaa
    10741 ccgatgaacc gggcgctgaa agaactgaat gcgcaaacgt ggtttgctgg cctgcggcgt
    10801 gaacaatccg gcagccgtgc caatttaccg gtgctggcaa ttcagcgtgg cgtatttaaa
    10861 gtgctgccga ttatcgactg ggataaccga actatttatc agtacctgca aaaacatggc
    10921 ctgaaatatc acccattatg ggacgaagga tatttatcgg tgggcgatac ccatacaacc
    10981 cgtaaatggg aacccggcat ggcggaagaa gaaacgcgtt tctttggctt aaaaagggaa
    11041 tgtggattac acgaagggta aatattgccg gataaattaa acgcctctgt cagaaatgat
    11101 gggggcgttt ttattttgcg agcagcattc cggcatttaa aggtgaacat cgcagggaaa
    11161 attaacaggt atagaatggc gttgggtgct tgaggctgtc tggcgttaag cattcgcgag
    11221 gttccagatg gacaaaagcc ccaggcgata tttctatcaa cctgaggctc gcgttcgaac
    11281 gtagcaattc ggatgttagt ctcttctttg cagggaggca agacatgctg acaaaatatg
    11341 cccttgtggc aatcatcgta ctgtgttgta cagtactggg attcacgttg atggtaggtg
    11401 actcgttatg tgagttgagt atcagagaac gtggtatgga gtttaaggca gttctcgctt
    11461 acgaatcgaa gaagtagcca ccacgcgggg gtaaaacccc gcgtactgaa ttgtgatggt
    11521 ttggtctcct gcaccctttt tttaaattta tattttttat gtgaatatat agtctctatt
    11581 tattattatg aatttcaaat gaaaatcatt aatttattca taatgtaaat gttttattgg
    11641 ctgatatata tcatagatag tttttaaaat atgcgtaaat atcctttaag tttactgaag
    11701 gataaaaata ttgtgacttt ctttgatttc tggggaaaaa accgacgtgg cgagaaagag
    11761 ggtatcgacg actatcacct tctttgctgg catttgctgg atgtggccgc aatgggctat
    11821 ttaatggtta aaagaaattg cttcgggctg gctgattatt ttcgtcaatt agggatttct
    11881 gacaaggaac aggcagctcg atttttcgcc tggttgctgt gctggcacga tattggaaaa
    11941 tttgcccgct cttttcaaca actttacctg ccccctgaat tcaagattca ggaaggtgcc
    12001 agaaaaaatt acgaaaaaat ctctcattca acgctgggct actggttgtg gaatcattat
    12061 ttaagtgaat gtcaggagtt acttccttca tcttcgctct ctccgcgcaa acttaggcga
    12121 gtgatagaaa tgtggatgcc cgtgacgaca gggcatcatg gtcggccccc tgatcgtatg
    12181 gatgaattgg ataattttct gcctgaagac aaagctgcag cacgagattt tctccttgaa
    12241 atcaagccac tgtttccgcg aatagagatc cccacattct gggatgatga cgagggcgtt
    12301 gaacttataa aacaactttc ctggtatatc tctgcaaccg tcgtacttgc agactggaca
    12361 gggtcgtcaa cgcgattttt tccacgcgtc gcacaggcaa tggatattga agattactgg
    12421 caaaaggctt tagttcaggc tcaaaacgca ttaaccgtct ttccgccaca agcagaaacc
    12481 gcgcctttca ccggaattaa tacgctgttt ccttttattg agaatacgac accgttacag
    12541 caaaaggtgc tggatctgga tatcaatcag ccaggaccac agttgtttat tctggaagat
    12601 gtgaccggcg caggtaaaac agaagcggcg cttatcctgg cgcacaggtt gatggctgcg
    12661 ggtaacgctc aggggctgtt ttttggcctg ccaacaatgg caacggccaa tgccatgtac
    12721 gatcgactgg tcaaaacctg gcttgctttc tattcgccag aggtgttccc cgctccagcg
    12781 gagataaacc ggcgcaatac ggtgcggttt tcccggaagt aaaccggacg cactggatgc
    12841 gatgatgggt atcacttaga attccccgcc cctgcggtag aactcccagc tcccattttc
    12901 aaacccatca agacgccttc gccagctcct tcaccaacgg tagcattatc cgcataacgt
    12961 cacggcagcg acgttctatt cttccaggaa gtgccttatc aatatgttgt tgattatcca
    13021 gtcttacgtc atgccagcta tttcccgccg ggaaggcagg tgtttttgcg cgttgctgat
    13081 aaccatcctt atttccaaga ttccagttag tcgcttccac cgaaagtaca gcaatgcccg
    13141 ctttgtcgaa tatttctgcg tcattacaac ccccagtgcc tttcggataa tttttattca
    13201 aacccggatt ggtcgttgcg gctattccgt gactgcgtgc aattgccagc gccctgtcgc
    13261 gcgttaattt ccttactgct tcaggggttt ttacaccgct gttgaaatac aatttatcgc
    13321 caacaattaa gttatcgaga ttaatcacca gcagcgtatt tttcttttcg gtgtcactca
    13381 tccgcttgag taaattctca gcgcctaatt ttccttcctc ttcgccgctg gtcgccacaa
    13441 atcgaatacc atactcggta ggcgtatttt tcaggcgttc tgccagttcc agcatgacac
    13501 ctaaacctgc ggcgttatca tctattcctt gtaacgtcag tccgccgaga ttggcatctg
    13561 cgtcagcatc gctcagcggg gcgtaagtat ccagatgcgc cataatgatg atctgctgcg
    13621 gcgctttgcc ttcatgagcg gcaatcaccg tacttcctgt cacgttatgc cagttcttgc
    13681 gattatcgcg ggcggtataa atataccgac tattaaatgt ccgaatatca ctgcgataac
    13741 ccatttgctg aaactgttgg cgaatataat cggcagataa catttctgct ggagttccgg
    13801 tcatgcgtcc cggaaagaaa gtagcaatat gtcgtgcctg ggtattagca aaatcgccag
    13861 gtttaggtga cgaggcgtgt acggggagaa taaagcatac gccgagcgcc agggcagcgg
    13921 tacggtggcg caatgcggaa aacatagtga gtccttaaat accatgcaaa tttttttacc
    13981 gccatagtat gaaactgccg ctgcgctaaa acaatttcaa atcttcctaa acgcccgaaa
    14041 tccggtgcct taagcacttt ttgatattag ctttgccaaa tcgttattcc gttaaggaac
    14101 tactcattct aattggtaat ttcattcgtt ctcttacgct ccctatagtc gaaacatctg
    14161 atggcaagaa aatagcggta ttgcaaagga acggttatgg ctcaaaaacg acttactcac
    14221 ctgcggcaac tggaggcgga aagcatccac attattcgcg aggtggcggc agaattctca
    14281 aatccggtga tgctctactc tatcggtaaa gattccagcg tcatgctgca tctggcgcgc
    14341 aaggcgtttt atccaggtac gctgcctttc ccgttgctgc atgtcgatac cggctggaaa
    14401 ttccgcgaga tgtatgagtt ccgcgatcgt acggcgaaag cctacggctg cgagctgctg
    14461 gtgcataaaa acccggaagg cgtggcgatg gggattaatc cattcgtgca cggcagcgcg
    14521 aaacataccg atattatgaa aactgaaggc ctgaaacagg cgctgaacaa atacggtttt
    14581 gatgccgcct tcggtggtgc gcgccgtgac gaagagaaat cccgcgctaa agagcgaatc
    14641 tactctttcc gtgaccgctt ccatcgctgg gatccgaaaa atcagcgccc ggagctgtgg
    14701 cacaactaca acgggcaaat taacaaaggc gaaagcatcc gcgtcttccc gctctctaac
    14761 tggaccgagc aggatatctg gcaatacatc tggctggaaa atatcgacat tgtgccgctg
    14821 tatctcgctg cggaacgtcc ggttctggaa cgcgacggta tgttgatgat gattgatgac
    14881 aaccgtatcg acctgcaacc gggcgaagtg atcaaaaaac ggatggtgcg tttccgtacg
    14941 ctgggctgct ggccgctgac cggtgcggtg gagtcaaatg cacaaacact gccggaaatc
    15001 atcgaagaga tgctggtttc caccaccagt gaacgtcagg gtcgcgtgat tgaccgcgac
    15061 caggcgggat ccatggagct gaaaaaacgt caggggtatt tttaagatga acaccgtact
    15121 tgcacaacaa atcgccaatg aaggcggcgt cgaagcctgg atgattgcgc aacaacataa
    15181 aagcctgctg cgttttctga cctgtggtag cgtcgatgac ggcaaaagta ccctgattgg
    15241 tcgtctgctg cacgataccc gccaaatcta cgaagatcag ctctcatcgc tgcataacga
    15301 cagtaagcgt cacggaaccc agggcgaaaa gctggatctg gctctgctgg tggacggcct
    15361 gcaagctgag cgcgaacagg gcatcactat tgatgtggcc taccgctatt tctctaccga
    15421 gaagcgtaaa tttattatcg ccgacacccc agggcacgag cagtacactc gcaatatggc
    15481 gactggcgca tcgacatgtg aactggcgat cttactgatt gatgcccgta aaggcgtgct
    15541 cgatcaaacc cgtcgtcaca gttttatctc cacactgttg gggatcaaac atctggtcgt
    15601 ggcgatcaac aaaatggatc tggtggatta cagtgaagag acgttcaccc gtattcgtga
    15661 agattatctg acctttgccg ggcagctgcc gggtaatctg gatatccgct ttgtgccgct
    15721 ctccgcactg gaaggtgaca acgtggcttc gcaaagtgaa agtatgccgt ggtacagcgg
    15781 tctgacactg ctcgaagtgc tggaaaccgt ggagatccag cgagtggtgg atgcccagcc
    15841 aatgcgcttc ccggtgcagt acgttaaccg cccaaatctc gattttcgtg gctacgccgg
    15901 aatgctggca tccggtcgcg tggaagtcgg gcaacgtgta aaagtgctgc cctctggtgt
    15961 ggaatcaaac gtcgcgcgga tcgtgacttt tgatggtgat cgcgaagaag tctttgccgg
    16021 agaagcgatc accctggtgc tgacggatga gatcgatatc agccgtggtg atctgctgct
    16081 ggcggcagac gaagcgttac cggcggtgca gagtgcgtcg gtggatgtgg tatggatggc
    16141 ggaacagccg ctttccccgg gccagagtta tgacatcaaa attgccggta agaagacgcg
    16201 tgctcgtgtt gatggcattc gttatcaggt tgatattaat aaccttaccc aacgcgaagt
    16261 tgaaaacctg ccgctgaacg gcatcggcct ggtggatctc acttttgacg agccactggt
    16321 gttagatcgc tatcagcaaa atccggttac cggtgggctg atttttatcg atcgcctgag
    16381 caatgtgacc gtgggtgccg gtatggtgca cgagccagtt agccaggcaa cagctgcgtc
    16441 atctgaattc agtgcattcg aactggaatt gaatgccctg gttcgtcgcc actttccgca
    16501 ctggggcgcg cgcgatttgc tgggggataa ataatggcgc tgcatgacga aaacgtcgtc
    16561 tggcatagtc atccggtcac tgtgcaacaa cgcgaactac accacggtca tcgtggtgta
    16621 gtgctgtggt ttaccggcct ctccgggtcc ggtaaatcaa cggtcgccgg ggcgctggag
    16681 gaggcgttac ataaactcag cgtcagtacg tatctgctgg atggcgacaa tgttcgccac
    16741 ggattatgca gcgatctcgg ttttagcgat gccgatcgta aagagaatat ccgtcgcgtc
    16801 ggtgaagtgg cgaatttgat ggttgaagcc ggactggtgg tgctgaccgc atttatctcg
    16861 ccacaccgcg ccgaacgcca gatggttcgc gaacgcgtag gagaagggcg ctttattgaa
    16921 gtgtttgtcg atacgccgct ggcgatttgc gaagcccgcg atccaaaagg cttatataag
    16981 aaagcgcgtg ccggtgaact gcgcaacttt acgggaatag attccgttta cgaagcgcct
    17041 gaatcggcag aaattcatct caatggtgaa caattagtaa caaatttggt acagcaatta
    17101 ttagatctgt tgagacagaa cgatattatc agatcctgag acaccaccgg gtttccatgc
    17161 ccggttcgtc aaagtaacag gatcaaatat gcgtaatagc cataacatta cactaacaaa
    17221 taacgacagc cttactgagg atgaagaaac tacatggtca ctgcctggtg ccgtggtcgg
    17281 ttttatctcc tggttatttg cgctggcgat gccgatgttg atttatggct ctaacacgct
    17341 gttcttcttt ctctacacct ggcctttctt tctggcactg atgcctgtcg cggtagtggt
    17401 ggggattgcg ctgcattcat tgatggacgg aaagctacgc tacagtattg ttttcaccct
    17461 ggtgacagta ggcattatgt ttggcgcact gtttatgtgg ctactgggct aatttgtact
    17521 ttcttgtctc ctctgttcat aattcaaacc gtaactaata atgagattat gttctgcacg
    17581 ccctgggtat acgtaacaat gggcaaatgt ggtacatttg cccgcgttgt cgcggtatcc
    17641 ccaacagagg atgtagagtc gtcttcggat gcatgggatg atgatgcagt ttttcagggg
    17701 gcaggatggg taaactaacg ctgctgttgc tggctattct ggtctggcta cagtattcgc
    17761 tgtggttcgg taagaacggt atacatgact atacccgcgt caatgatgat gtggcggcac
    17821 tgcaagctac aaacgcgaaa cttaaagcgc gaaacgatca actttttgcc gaaattgacg
    17881 atctcaatgg cggccaggag gcgctcgaag agcgtgcgcg taatgaactc agcatgacca
    17941 ggccgggcga aactttttat cgtctggtgc ctgacgcgtc gaagcgcgca cagtctgcgg
    18001 ggcaaaacaa tcgataaatc agcccgggaa ttaacatggc aaccactcat ttggatgttt
    18061 gcgccgtggt tccggcggcc ggatttggcc gtcgaatgca aacggaatgt cctaagcaat
    18121 atctctcaat cggtaatcaa accattcttg aacactcggt gcatgcgctg ctggcgcatc
    18181 cccgggtgac acgtgtcgtc attgccataa gtcctggcga tagccgtttt gcacaacttc
    18241 ctctggcgaa tcatccgcaa atcaccgttg tagatggcgg tgatgagcgt gccgattccg
    18301 tgctggcagg tctgaaagcc gctggcgatg tgcagtgggt attggtgcat gacgccgctc
    18361 gtccttgttt gcatcaggat gaccttgcgc gattgttggc gttgagcgaa accagccgca
    18421 cgggagggat cctcgccgca ccagtgcgcg atactatgaa acgtgccgaa ccgggcaaaa
    18481 atgccattgc tcataccgtt gatcgcaacg gcttatggca cgcgctgacg ccgcaatttt
    18541 tccctcgtga gctgttacat gactgtctga cgcgcgctct aaatgaaggc gctaccatta
    18601 ccgacgaagc ctcggcgctg gaatattgcg gatttcatcc gcagttggtc gaaggccgcg
    18661 cggataacat taaagtcacg cgcccggaag atttggcact ggccgagttt tacctcaccc
    18721 gaaccatcca tcaggagaat acataatgcg aattggacac ggttttgacg tacacgcctt
    18781 tggcggtgaa ggcccaatta tcattggtgg cgtacgcatt ccttacgaaa aaggattgct
    18841 ggcgcattct gatggcgacg tggcgcttca tgcgttgacc gatgcattgc ttggcgcggc
    18901 ggcgctgggg gatatcggca aactgttccc ggataccgat ccggcattta aaggtgccga
    18961 cagccgcgag ctgctacgcg aagcctggcg tcgtattcag gcgaagggtt atacccttgg
    19021 caacgtcgat gtcactatca tcgctcaggc accgaagatg ttgccgcaca ttccacaaat
    19081 gcgcgtgttt attgccgaag atctcggctg ccatatggat gatgttaacg tgaaagccac
    19141 tactacggaa aaacttggat ttaccggacg tggggaaggg attgcctgtg aagcggtggc
    19201 gctactcatt aaggcaacaa aatgattgag tttgataatc tcacttacct ccacggtaaa
    19261 ccgcaaggca ccgggctgct gaaagccaat ccggaagact ttgtggtggt ggaagatttg
    19321 ggctttgagc ctgatggtga aggtgagcat attctggtta gaatcctcaa aaacggctgc
    19381 aatacccgtt ttgtggcgga tgcactggcg aaattcctga aaattcattc ccgtgaagtc
    19441 agcttcgctg ggcaaaaaga caaacatgct gtaacggaac agtggttatg tgctcgcgtg
    19501 ccgggcaagg aaatgcccga tctgagcgcc tttcaactgg aaggctgcca ggtgctggag
    19561 tatgcgcggc acaagcgcaa gctgcgttta ggggcgctga aaggtaacgc ctttaccctg
    19621 gttctgcgcg aagtgagcaa tcgcgatgac gttgaacaac gtctgatcga tatttgcgta
    19681 aaaggtgtac cgaactactt cggtgcccaa cgttttggga ttggcggtag caacttgcag
    19741 ggcgcgctgc gctgggcgca aaccaatact ccggtgcgcg atcgcaataa acggagtttt
    19801 tggttgtcgg cagcccgcag tgcgttgttt aatcagattg ttgctgagcg cctcaaaaaa
    19861 gcagacgtta atcaagttgt tgacggcgat gcgctacaat tagccggacg tggtagctgg
    19921 tttgtagcaa ccaccgaaga actggcggaa ttacagcgtc gcgtcaacga taaagagctg
    19981 atgataaccg ccgcattgcc gggcagtggc gaatggggaa ctcagcgtga agcgctggca
    20041 ttcgaacaag cagctgtcgc cgcagaaact gaattacaag ctttactggt gcgcgaaaaa
    20101 gttgaagccg cgcgcagagc gatgctgctg tatccgcaac aattaagctg gaactggtgg
    20161 gatgacgtca ccgtagagat ccgtttctgg cttccggcgg gtagttttgc aaccagcgtt
    20221 gtcagggaac ttatcaacac aacaggtgat tatgcgcata ttgctgagta attatgacgg
    20281 ggtacatgca cccggtatac aaacgctggc gaaagccttg cgtgagtttg ctgacgttca
    20341 ggtggtcgcc cccgatcgta accgcagcgg cgcttcaaat tctctgacac tggaatcctc
    20401 cctgcgcacg tttacctttg aaaatggtga tattgctgtg caaatgggaa ccccgaccga
    20461 ttgtgtctat cttggcgtga atgctctgat gcgtccgcgc ccggacattg ttgtgtccgg
    20521 aattaacgcc gggccgaatc tgggggatga tgttatttat tccggtacgg tagccgccgc
    20581 gatggaaggc cgtcatttag gttttccggc gctggccgtc tcgcttgacg ggcataaaca
    20641 ttacgacact gccgcggcgg taacctgttc aattttgcgc gcactgtgta aagagccgct
    20701 gcgcaccgga cgtattctta atattaacgt tcaggattta cccttggatc aaatcaaagg
    20761 tattcgcgtg acgcgctgcg gtacacgaca tccggcagat caggtgatcc cgcagcaaga
    20821 tccgcgcggc aatacgctgt actggattgg cccgccgggc ggtaaatgtg atgccggtcc
    20881 ggggaccgat tttgctgcgg tagatgaggg ctatgtctcc atcacgccgc tgcatgtgga
    20941 tttaactgcg catagcgcgc aagatgtggt ttcagactgg ttaaacagcg tgggagttgg
    21001 cacgcaatgg taagcagacg cgtacaagca cttctggatc aattacgtgc gcaaggtatt
    21061 caggatgagc aggtgctgaa tgcacttgcc gccgtgccgc gtgaaaaatt cgttgatgaa
    21121 gcgtttgaac aaaaagcctg ggacaatatc gccttgccga taggtcaggg gcagacaatt
    21181 tcgcagccgt atatggtggc gcgaatgacc gaattactcg agctgacgcc gcagtcgcgg
    21241 gtgctggaaa ttggcactgg ttcgggatat caaacggcaa tcctggcgca tcttgtccag
    21301 catgtttgct cggttgaacg gattaaaggc ttgcagtggc aggcacgtcg ccgcctgaaa
    21361 aatcttgatt tacataatgt ttcaacccgt catggtgatg gatggcaagg ttggcaggca
    21421 cgtgcgccgt ttgacgctat cattgttacg gcggcaccgc cggaaattcc aactgcgcta
    21481 atgacgcagc tggacgaagg cgggattctc gtcttacccg taggggagga gcaccagtat
    21541 ttgaaacggg tgcgtcgtcg gggaggcgaa tttattatcg ataccgtgga ggccgtgcgc
    21601 tttgtccctt tagtgaaggg tgagctggct taaaacgtga ggaaatacct ggatttttcc
    21661 tggttatttt gccgtaggtc agcgtatcgt gaacatcttt tccagtgttc agtagggtgc
    21721 cttgcacggt aattatgtca ctggttatta accaattttt cctgggggat aaatgagcgc
    21781 gggaagccca aaattcaccg ttcgccgcat tgcggctttg tcactggttt cgctatggct
    21841 ggcaggctgt tctgacactt caaatccacc ggcaccggtc agctccgtta atggcaatgc
    21901 gcctgcaaat actaattctg gtatgttgat tacgccgccg ccgaaaatgg ggacgacgtc
    21961 tacagcgcag caaccgcaaa ttcagccggt acagcagcca caaattcagg ctactcaaca
    22021 accgcaaatc cagccagtgc agccagtagc tcagcagccg gtacagatgg aaaacggacg
    22081 catcgtctat aaccgtcagt atgggaacat tccgaaaggc agttatagcg gcagtaccta
    22141 taccgtgaaa aaaggcgaca cacttttcta tatcgcctgg attactggca acgatttccg
    22201 tgaccttgct cagcgcaaca atattcaggc accatacgcg ctgaacgttg gtcagacctt
    22261 gcaggtgggt aatgcttccg gtacgccaat cactggcgga aatgccatta cccaggccga
    22321 cgcagcagag caaggagttg tgatcaagcc tgcacaaaat tccaccgttg ctgttgcgtc
    22381 gcaaccgaca attacgtatt ctgagtcttc gggtgaacag agtgctaaca aaatgttgcc
    22441 gaacaacaag ccaactgcga ccacggtcac agcgcctgta acggtaccaa cagcaagcac
    22501 aaccgagccg actgtcagca gtacatcaac cagtacgcct atctccacct ggcgctggcc
    22561 gactgagggc aaagtgatcg aaacctttgg cgcttctgag gggggcaaca aggggattga
    22621 tatcgcaggc agcaaaggac aggcaattat cgcgaccgca gatggccgcg ttgtttatgc
    22681 tggtaacgcg ctgcgcggct acggtaatct gattatcatc aaacataatg atgattacct
    22741 gagtgcctac gcccataacg acacaatgct ggtccgggaa caacaagaag tgaaggcggg
    22801 gcaaaaaata gcaaccatgg gtagcaccgg aaccagttca acacgcttgc attttgaaat
    22861 tcgttacaag gggaaatccg taaacccgct gcgttatttg ccgcagcgat aaatcggcgg
    22921 aaccaggctt ttgcttgaat gttccgtcaa gggatcacgg gtaggagcca ccttatgagt
    22981 cagaatacgc tgaaagttca tgatttaaat gaagatgcgg aatttgatga gaacggagtt
    23041 gaggtttttg acgaaaaggc cttagtagaa gaggaaccca gtgataacga tttggccgaa
    23101 gaggaactgt tatcgcaggg agccacacag cgtgtgttgg acgcgactca gctttacctt
    23161 ggtgagattg gttattcacc actgttaacg gccgaagaag aagtttattt tgcgcgtcgc
    23221 gcactgcgtg gagatgtcgc ctctcgccgc cggatgatcg agagtaactt gcgtctggtg
    23281 gtaaaaattg cccgccgtta tggcaatcgt ggtctggcgt tgctggacct tatcgaagag
    23341 ggcaacttgg ggctgatccg cgcggtagag aagtttgacc cggaacgtgg tttccgcttc
    23401 tcaacatacg caacctggtg gattcgccag acgattgaac gggcgattat gaaccaaacc
    23461 cgtactattc gtttgccgat tcacatcgta aaggagctga acgtttacct gcgaaccgca
    23521 cgtgagttgt cccataagct ggaccatgaa ccaagtgcgg aagagatcgc agagcaactg
    23581 gataagccag ttgatgacgt cagccgtatg cttcgtctta acgagcgcat tacctcggta
    23641 gacaccccgc tgggtggtga ttccgaaaaa gcgttgctgg acatcctggc cgatgaaaaa
    23701 gagaacggtc cggaagatac cacgcaagat gacgatatga agcagagcat cgtcaaatgg
    23761 ctgttcgagc tgaacgccaa acagcgtgaa gtactggcac gtcgattcgg tttgctgggg
    23821 tatgaagcgg caacactgga agatgtaggt cgtgaaattg gcctcacccg tgaacgtgtt
    23881 cgccagattc aggttgaagg cctgcgccgt ttgcgcgaaa tcctgcaaac gcaggggctg
    23941 aatatcgaag cgctgttccg caagtaagta agcatctgtc agaaaggcca gtctcaagcg
    24001 aggctggcct ttttcttttg tttgggaagc ttgtgttttt gtcgcctgga taagacacgt
    24061 cagcgtccta cccggcaatc agcattctat gtgctcatca tcttgcgcac caactgcata
    24121 aacagctcct gctcttctcc actcaaacgc cccaaaaatt cctcatccac gctgtcacca
    24181 ataggtatcg ccgcagcaag tagcttttct ccttcggcag tcagccagac aaagcgtcgc
    24241 cgcttgtcag cgacgtcatg ttcacgtctg actaagccac gattttccat tcttgccaac
    24301 atttctgcta gcgttgcttt ggtgctgact gctgcttcta tcagtgccac ctgttcaata
    24361 ccaggtttat cagcaatggc gcgcatcacc gcgtactgtg gtttggtcag gtcaggtaac
    24421 tcgtgctgcc agcgagccgt gtgctgctga aaaagctgtc gtaactgatg gaacgcttta
    24481 tttcgtaacg ccatgtaaac tccgggctaa ttctctgtcg ctatcatagc ggttttttag
    24541 tgacgaagaa gatgttgtta cttttcaatc cttgccgctg tggaaatgaa ggagtatgtt
    24601 aataataacg ttcgtatacg aataattaag agaataagca atgaaactga tcgtcgggat
    24661 gacaggggct accggtgcgc ctcttggtgt ggcattactg caagcgctgc gtgagatgcc
    24721 gaatgtcgag actcatctgg tgatgtcgaa gtgggcgaaa accaccattg aactggaaac
    24781 gccttacagc gctcgcgatg ttgctgccct cgcagacttc agccataacc cggcggatca
    24841 ggcggcgatc atctcatccg gttcttttcg taccgacggc atgatcgtta ttccgtgcag
    24901 tatgaaaacg ctcgccggta tccgcgctgg ttacgccgat ggcctggtag ggcgcgcggc
    24961 ggacgtcgtg ctcaaagaag gccgcaaact ggtgctggtg ccgcgtgaaa tgccgcttag
    25021 caccatccat ctcgaaaata tgctcgcact ttcacgcatg ggcgtggcga tggtgccgcc
    25081 gatgcctgcc ttttataacc atcccgaaac ggtagatgac attgtccacc atgtggtagc
    25141 ccgcgtgctg gatcaatttg gcctcgaaca tccccacgcc aggcgctggc aaggattgcc
    25201 gcaggcccgg aatttttctc aggagaatga ataatggcat ttgatgattt acgcagcttt
    25261 ttacaggcgc ttgatgacca cggccagtta ctgaaaatca gcgaagaagt gaacgctgag
    25321 ccggatctgg cagcagcagc taacgccacc gggcgtatcg gcgacggcgc gcccgcgctg
    25381 tggtttgata atattcgcgg ctttaccgat gcccgcgtgg cgatgaatac catcggttcc
    25441 tggcagaacc acgcgatttc ccttggcctg ccaccaaaca ccccggtcaa aaagcagatt
    25501 gatgagttta tccgccgctg ggataacttc ccgattgccc cggagcgccg cgccaatcca
    25561 gcctgggcgc agaacaccgt cgatggcgaa gagatcaacc tgttcgatat tctgccgctg
    25621 tttcgcctga atgatggcga tggcggtttc tatctcgaca aagcgtgcgt ggtttcccgc
    25681 gatccgctcg acccggataa cttcggcaag cagaacgtcg gcatctaccg catggaagtg
    25741 aagggcaagc gtaagctcgg cctgcaaccg gtgccgatgc acgatatcgc cctgcatctg
    25801 cataaagcag aagagcgcgg tgaagatctg ccgattgcga tcacgctcgg taacgatccg
    25861 atcatcacgc tgatgggggc cacgccgctg aaatacgatc agtctgagta tgaaatggca
    25921 ggcgcgctgc gtgaaagccc gtacccgatc gccaccgctc cgctgaccgg ttttgatgtg
    25981 ccgtggggtt cagaagtgat cctcgaaggg gttatcgaaa gccgtaaacg cgaaatcgaa
    26041 gggccgttcg gtgagtttac cgggcactac tccggtgggc gcaacatgac cgtggtgcgc
    26101 atcgataaag tctcttatcg cagcaaaccg attttcgaat cactctatct cggtatgccg
    26161 tggaccgaaa tcgactacct atgactaggt aatttattca agataaccgg gaagagatta
    26221 aaaaagacgg gaatagattg gaatcaacaa gttaggaact caaggttttc aagataaagc
    26281 gagtccaaaa atgttaaacg gttcgatatc aataaaacat tcaagataac tcacaggttc
    26341 ttacaatagc ctttaaagac cgttcaaaaa cgattcaggt gtttattata gcgctgatgg
    26401 gtgcaagcgc caaaaccaat gtaattttag cacagctagg cacttgcact gggttatgcg
    26461 ctggctgctt tctgaggtag atttaagtga tcgcccttag cttcggacaa cgcgtaaatc
    26521 tctctaacct tttcgtctgg caaattacca aggcttaacg ctatggctct acgaactcca
    26581 actagagcgt gcaactcttg gatgtgctca tccagaagaa tgagtaattg ctccgctcct
    26641 tttctcccaa gaacatcagc tacttgttgt ttttcttgtg gtttaagaaa tgacaagaac
    26701 attagaattt gagcatcaac attatccgca ttctcttttt tgggttcttt ggaatctttt
    26761 agttcaacat caccctctcc cgtcatgagc cattccaagg taacattttc gctttctgct
    26821 atctttttta actgatacgg gcgtggtatc ggtgcgtaat cccgcctgtt gtaataattt
    26881 tttaaagtgc cttcattgat cccccatgcc cgagcagccg catttcttga agggtatcgt
    26941 tctactaacc taaagattct ttcgataatc ggttcttttc catcggcagg aaaagaacct
    27001 tggggtgctt ttctcattct tggttccttt ctatctaatt cattgataaa taataaaatt
    27061 aaaaaataaa ccataaaaac accaaagaac cctttgcagg gaatctttgg tatcttaatc
    27121 tttatccata cggatatccg accggatatc cgcgcgggta attttgtagg ttaaaggaaa
    27181 gatgagacaa agagaagata gtgaccgcga ttggccttcg ctgaagattc agctggcgct
    27241 caaagataac gggtggtctt ccttccggga gctttcactc catgctggtt atgctcagga
    27301 ttcactgaaa gcggtagttc gcacaccaaa caaaaaatac gaacgcgtaa tttctgacgc
    27361 tataggtgta ccgccagaaa tcatctggcc cagccggtat cgtaacgatg gatttatcag
    27421 gaaggcgtcg tgatatgcag tcgtacgtta ccgtaaatga cctgctcggc gtaccaggaa
    27481 tgccagccac cacaaaaggc atccgacagg cgttacagcg tttttctcgt gatcttggtg
    27541 acgttagtcg ccgtcgggaa ggcacaaaag ccattgagta ccacattgat tgtctgcctg
    27601 aaataacacg aaaggcactg cgtgaacgct atgtggaaca gctggtggct acagagaaca
    27661 atgtttctga agtgaaagct gttaccagaa aaacacgcaa tcctgacgct gtccaggcaa
    27721 tcgaagcata tcgcggttct ccacaactga tggaagaacg cctgaatgcg ctgaccgaaa
    27781 accagcgctg ggtatctgaa gcaagagctg cgctggtggt ggaagtgctg aagctggaaa
    27841 gcgccggtaa ccccgggcga ctgaaagcca ttaactttct tgttgaaaaa gcccgtaaag
    27901 gtgagctgcc ggagcgcctg caacaggccg cagttaacgc caatgcaaaa cgtggcgcta
    27961 atcgtactat cagccgcgat ccactttacc agtgggttct gaaatacaac cagtcgcaga
    28021 acgccgccga acggctgctg ttactggcac cgggaaaacg cgacgaaata aaaccggaag
    28081 aaatcagctg gctgcctgaa tttctggcgc agtatcgcca ggtgaacggc agacccatgt
    28141 cagaagccta cgaggatttt gtcgcagaat ggcagcgtcg ccatgcggac gaaccgtaca
    28201 tgctggaggt gatgccttcc tatgatgttg tccggtatgc catgaaaaaa ctgccggaag
    28261 tggtgaaaca aaaagggcgc gttaccggca gtgaataccg tcagcttgaa ggattcaccc
    28321 gccgcgactg gacagctatg ccggtgaatt atgtctggat tggtgacggt cacggtatga
    28381 agctgaaatg tgcgcatccg atccacgggc gtccattctc accggaagtc acgtttgtga
    28441 tcgatggtgg aacacggttt gttgttggct ggagtcttga ccttgccgaa aacgtgttcg
    28501 ccgttgccgg tgcgatacag cacggcattc gcaaccacgg aaaaccgttc ctgtattact
    28561 cggataacgg ttccggtgaa acggctgaca tgctggacaa agaggtcgtg ggtattctgc
    28621 cacgcctggg gattaaacac cctaccggta ttgccggtaa tccacaggga cgcggcatta
    28681 ttgaacgcct gaaccggaca ctgccgatgc gtatagcgag gaggtatcgc acctattttg
    28741 gcaagggtgc tgaccgggaa tcgttgcgtg tacttaaccg tgatctgcgt tcagcgttta
    28801 acgccctgca acaggacaag cctctgaacg accgccagaa agcggcaatg cgcgagctgc
    28861 catcgtgggc ggagcttatt gaggctattc gtgaaggcgt ggaatggtac aacaaccgcc
    28921 cgcattctga gctgccgatg aaaccgaacg gacggcatta cagcccgaca gagttcagaa
    28981 aaaaacgcca ggcagaagaa gacaccgaaa ttgagtggct gagtgatctt gagctgcgcg
    29041 acatgttccg cccaatggtg gaacgaccgg taagacgctg cgaaattcag tggctgaaca
    29101 atatttacta cgcgccggag ttgcgtgacg aacacggtcg caaagtgctt atcagttatg
    29161 acattcatga cgccgagcga attacggtac gtcgcaagga cggcagcttt atctgtgagg
    29221 cgatatggaa cggcaataaa cgcgcagcct ttgctgtcag tgctgaatat cacaaacaac
    29281 agcagcgtat taaaggaatg cgcaaacgcg ctgaggaaaa aattcgtgat gctgaagatg
    29341 aaggtattca gattctggaa cacaaacagg ctgaaccctg gctgagtaat gtttatcgcc
    29401 cggttggtaa tgtggtggca gttcagcaac cggaatatga agaagagcat gacgaagaat
    29461 ttgaacgcga tttccggctg gggatgcaaa aactttttgc catgcaggaa gaagatgatc
    29521 cgctggcctg aataaaaaaa cagtccgcgg gaacggactg ttaaataaag gaaacaaagc
    29581 tgatttgaaa taaccgagga aatactatga ctgatattga tga
//
LOCUS       DAJTQQ010000044        29043 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000044 DAJTQQ010000000
VERSION     DAJTQQ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.044"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            109..1140
                     /gene="mdtN"
                     /locus_tag="OO309_002141"
     CDS             109..1140
                     /gene="mdtN"
                     /locus_tag="OO309_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR5847278.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1140..3182
                     /gene="mdtO"
                     /locus_tag="OO309_002142"
     CDS             1140..3182
                     /gene="mdtO"
                     /locus_tag="OO309_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR5847279.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            3179..4645
                     /gene="mdtP"
                     /locus_tag="OO309_002143"
     CDS             3179..4645
                     /gene="mdtP"
                     /locus_tag="OO309_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR5847280.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4843..5082
                     /locus_tag="OO309_002144"
     CDS             4843..5082
                     /locus_tag="OO309_002144"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5847281.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5092..5262
                     /locus_tag="OO309_002145"
     CDS             5092..5262
                     /locus_tag="OO309_002145"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5847282.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5311..6723
                     /gene="fdhF"
                     /locus_tag="OO309_002146"
     CDS             5311..6723
                     /gene="fdhF"
                     /locus_tag="OO309_002146"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR5847283.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6724..6990
                     /locus_tag="OO309_002147"
     CDS             6724..6990
                     /locus_tag="OO309_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HCR5847284.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7084..7773
                     /locus_tag="OO309_002148"
     CDS             7084..7773
                     /locus_tag="OO309_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5847285.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OO309_002149"
     CDS             complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OO309_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR5847286.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OO309_002150"
     CDS             complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OO309_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR5847287.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OO309_002151"
     CDS             complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OO309_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR5847288.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10475..12193)
                     /locus_tag="OO309_002152"
     CDS             complement(10475..12193)
                     /locus_tag="OO309_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR5847289.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OO309_002153"
     CDS             complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OO309_002153"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR5847290.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OO309_002154"
     CDS             complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OO309_002154"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR5847291.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OO309_002155"
     CDS             complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OO309_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR5847292.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OO309_002156"
     CDS             complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OO309_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR5847293.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16274..18232
                     /gene="acs"
                     /locus_tag="OO309_002157"
                     /pseudo
     CDS             16274..18232
                     /gene="acs"
                     /locus_tag="OO309_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            18432..18746
                     /locus_tag="OO309_002158"
     CDS             18432..18746
                     /locus_tag="OO309_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR5847294.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18743..20392
                     /gene="actP"
                     /locus_tag="OO309_002159"
     CDS             18743..20392
                     /gene="actP"
                     /locus_tag="OO309_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR5847295.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20570..21862
                     /locus_tag="OO309_002160"
     CDS             20570..21862
                     /locus_tag="OO309_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5847296.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22016..23665)
                     /locus_tag="OO309_002161"
     CDS             complement(22016..23665)
                     /locus_tag="OO309_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR5847297.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OO309_002162"
                     /gene_synonym="yjcD"
     CDS             complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OO309_002162"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR5847298.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="OO309_002163"
     CDS             complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="OO309_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR5847299.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26421..26744
                     /gene="soxS"
                     /locus_tag="OO309_002164"
     CDS             26421..26744
                     /gene="soxS"
                     /locus_tag="OO309_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR5847300.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="OO309_002165"
                     /pseudo
     CDS             complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="OO309_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28753..29034
                     /locus_tag="OO309_002166"
     CDS             28753..29034
                     /locus_tag="OO309_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR5847301.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7284 a         7776 c         7352 g         6631 t
ORIGIN      
        1 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
       61 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
      121 ccgaaaaaag cacctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt
      181 gcccttgttt tcgttatctg gcgcgtagac agtgcgccat caactaatga cgcttacgcg
      241 tcagcagata ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc
      301 accgacaacc aggcggtgaa acagggcgat ttgctgttcc gcatcgaccc gcgcccgtac
      361 gaagccaacc tggcgaaagc cgaagcctcc ctcgcggcgc tggataagca aattatgctc
      421 acccagcgta gcgttgacgc ccaacagttt ggtgccgact cggttaatgc cacggtagaa
      481 aaagcccgtg ccgccgcgaa acaggccaca gatacattac gccgcaccga gccattactg
      541 aaagagggtt ttgtctcagc agaagacgtt gaccgtgcaa gaacggcgca gcgcgccgca
      601 gaagcggatc ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc
      661 gtcgatgcgc tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaacta
      721 catctggaaa tgacgaccgt tcgcgcgcca tttgatggcc ggatcatttc cctcaaaacc
      781 tccgtcgggc aatttgcttc tgccatgcgc cctattttta ccctcatcga cactcgtcac
      841 tggtacgtga tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc
      901 gcaacgattc gcctgatgag tgacagcggc aaaaccttcg agggtaaagt ggattcgatt
      961 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc aaaagtgtct
     1021 cgttctatta actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa
     1081 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
     1141 tgagcgcgct caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag
     1201 agttaagcga gcggcgaccc ggccgcgtgc cgcagatcgt gcaactctgg gtaggctgcc
     1261 tgctggtgat tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag
     1321 tgctgtttta cggtattcag tcgaacgcgt tttacaccaa atttgtggcg atcttgtttg
     1381 tggttgccac ggtgctggag atcggcagcc tgtttttgat ctacaaatgg tcatacggcg
     1441 aaccgttgat ccgattgatc atcgccgggc cgatcctgat gggctgcatg tttttgatgc
     1501 gcacccatcg gctgggactg gtctttttcg ctgtcgccat ttacgggcaa accttccccg
     1561 ccatgctcga ctatccggaa gtagtcgtgc gcttaacgct gtggtgtatc gttgttggcc
     1621 tctatccaac cttgctgatg acgttaatcg gcgtgctgtg gtttcccaat cgtgccatta
     1681 cgcaaatgca tcaagcgctt aatgatcggc ttgatgatgc cattagccac ctgacggaca
     1741 gcctcgcacc gctacccgaa acgcggattg aaagagaggc gctggcgctg caaaaactga
     1801 atgtcttttg tctcgcggac gatgccaact ggcgaactca aagcgcatgg tggcaaagct
     1861 gcgtggcaac ggtaacctac atttactcga cgctgaatcg ctacgatccc acctcttttg
     1921 ctgattctca ggcaattatt gaatttcggc aaaaattagc ctcagaaatc aacaagctgc
     1981 agcatgccgt tgctgaaggt cagtgctggc aaagcgactg gcggctcagt gaaagtgaag
     2041 cggtggcggc acgggaatgt aacctggaga atatctgcca gacgttgtta caactgggcc
     2101 agatgaaccc gaatacgccg ccaacgcccg ccgccaaacc gccatcaatg gtcgccgatg
     2161 cttttaccaa tccagactat atacgctacg cggtaaaaac attgctcgcc tgtttgatct
     2221 gttacacctt ttacagcggc gtggactggg aaggcattca cacctgtatg ctgacctgcg
     2281 tgatcgtcgc taatccaaat gtcggttcgt cgtaccagaa gatggtgctg cgttttggcg
     2341 gggccttttg cggcgcgatt ctggcgctgt tattcacgct actggtcatg ccctggctgg
     2401 acaatattgt cgaattgctg tttgtgctgg caccgatttt cctgttgggc gcatggattg
     2461 ccaccagctc tgaacgctct tcttatatcg gcacacagat ggtggtcacc ttcgcgctcg
     2521 ccacgctcga aaacgttttt ggcccagtgt acgacctggt ggaaattcgc gatcgcgccc
     2581 tgggtatcat cattggtacc gtggtgtccg cgatgattta cacctttgtc tggcctgaaa
     2641 gtgaagcgcg cacgctgccg caaaaactgg ctggcgcgct gggtatgtta agtaaagtaa
     2701 tgcggatccc acgccagcag gaagtcacgg ctctgcgcac ttatctgcaa attcgtatcg
     2761 gtctgcatgc ggcgtttaat gcctgtgaag agatgtgcca acgcgtggcg ctggagcgtc
     2821 aactggacag cgaagaacgc gccttactga ttgaacgttc gcaaacggtt attcgtcagg
     2881 gccgcgatat ccttcacgcc tgggatgcca cctggaattc ggcgcaggcg ctggataacg
     2941 cactacagcc ggacagagca ggtcagtttg ccgacgccct ggagaaatac gctgccggtc
     3001 tggcaaccgc actcagccgt tctcctcaaa taacgcttga agagacaccc acctcgcagg
     3061 ccatcctgcc caccttatta aaacaggagc aacacgtctg ccagcttttc gcccgcttgc
     3121 cagactggac agccccggca ttaacgcccg ccacggaaca ggcacaagga gccacgcaat
     3181 gatcaatcgt caactttcac gtctgctgtt gtgcagcatt ctcggcagca cgacgctgat
     3241 ttccggctgt gccctggtac gtaaggattc tgcgcctcat caacagctca aaccggaaca
     3301 aatcaaactg gccgatgata ttcatcttgc cagctccggc tggccgcagg cgcagtggtg
     3361 gaaacaactc aatgacccgc agttggatgc cctgatccaa cggacgctaa gtggttcaca
     3421 caccctcgcc gaagcgaaac tgcgggaaga aaaagcgcaa tcgcaggccg atttgttaga
     3481 tgccggttca cagttgcagg tggcagcgtt agggatgctt aaccgccaac gtgtctcggc
     3541 gaacggcttt ttaagccctt atgcgatgga tgcgcccgca ctggggatgg acgggccgta
     3601 ctatacggaa gccacagtag gtttgtttgc cggactggat cttgatttgt ggggtgtgca
     3661 tcgctcagcg gttgccgccg ccattggcgc gcataatgcc gcgctggcag aaaccgcagc
     3721 agtagagcta tcgctgacca cgggcgtagc gcagctttat tacagtatgc aggccagcta
     3781 tcagatgctc gatctgttag aacaaactcg cgatgtgatt gattacgcgg tgaaagcgca
     3841 ccaaagtaaa gtggcgcacg gtctggaagc acaagtgcct ttccacggcg cgcgggcaca
     3901 gattctggcg gtcgataaac aaattgctgc cgtcaaaggg caaatcaccg aaacgagaga
     3961 atctctgcgc gcattgattg gcgcgggagc cagcgatatg ccggagatca aacctgtggc
     4021 attaccacga gtccagaccg gcattccggc gacactctct tatgagttgc tcgccagacg
     4081 cccggatctg caagccatgc gctggtatgt tcaggcgtca ttagatcagg tggattccgc
     4141 gcgggcgttg ttctatccga gctttgatat caaagcgttt ttcggccttg atgccatcca
     4201 cctggatacc ttattcaaaa aaaccagtcg ccagttcaac ttcatcccgg gtctgaaatt
     4261 gccgctgttt gacggtggac ggttgaatgc caatctcgaa ggcacgcgcg ccgccagcaa
     4321 catgatgatt gaacgttaca accagtcagt actgaacgcg gtgcgtgacg ttgccgtcaa
     4381 cggcacgcgt ctgcaaacgc tcaacgacga gcgagaaatg caggctgaac acgtggaagc
     4441 aacgcgcttc acccagcgcg ctgccgaggc cgcctatcag cgcggcttaa ccagccgctt
     4501 acaggccact gaagcccggt tgccagtact tgccgaagag atgtcattac tgatgctgga
     4561 cagccgccgg gtgatccaaa gcattcagtt gatgaaatcg ctgggcggcg ggtatcaggc
     4621 ggctcccgtc gtcgagaaaa aataaaatgt ctgccgcgtg atggctgtca cgcggtattt
     4681 cgtttcgtca cgtcaaaact gacgacagcc tgtttttcgt cagagttttg aataaatagt
     4741 gcccgtaata tcagggaatg accccaaata aaatgtggca taaaagatgc atactgtagt
     4801 cgagagcgcg tatgcgtgat ttgattaact ggagcgagac cgatgaaaaa agtcgtcacg
     4861 gtttgcccct attgcgcatc aggttgcaaa atcaacctgg tcgtcgataa cggcaaaatc
     4921 gtccgggcgg aggcagcgca ggggaaaacc aaccagggta ccctgtgtct gaagggttat
     4981 tatggctggg atttcattaa cgatacccag atcctgaccc cgcgcctgaa aacccccatg
     5041 atccgtcgcc agcgtggcgg caaactcgaa cctgtttcct gagatgaggc actgaattac
     5101 gttgccgagc gcctgagcgc catcaaagag aagtacggtc cggatgccat ccagacgacc
     5161 ggctcctcgc gtggtacggg taacgaaacc aactatgtaa tgcaaaaatt tgcgcgcgcc
     5221 gttattggta ccaataacgt tgactgctgc gctcgtgtct gacacggccc atcggttgca
     5281 ggtctgcacc aatcggtcgg taatggcgca atgagcaatg ctattaacga aattgataat
     5341 accgatttag tgttcgtttt cgggtacaac ccggcggatt cccacccaat cgtggcgaat
     5401 cacgtaatta acgctaaacg taacggggcg aaaattatcg tctgcgatcc gcgcaaaatt
     5461 gaaaccgcgc gcattgctga catgcacatt gcactgaaaa acggctcgaa catcgcgctg
     5521 ttgaatgcga tgggccatgt cattattgaa gaaaatctgt acgacaaagc gttcgtcgct
     5581 tcccgtacag aaggctttga agagtatcgt aaaatcgttg aaggctacac gccggagtcg
     5641 gttgaagata tcaccggtgt cagcgccagt gagattcgtc aggcggcacg gatgtatgcc
     5701 caggcgaaaa gcgccgccat cctgtggggc atgggtgtaa cccagttcta ccagggcgtg
     5761 gaaaccgtgc gttctctgac cagcctcgcg atgctgacca gtaacctcgg taagccgcat
     5821 gcgggtgtaa acccggttcg tggtcagaac aacgtgcagg gtgcctgcga tatgggcgcg
     5881 ctgccggata cgtatccggg ataccagtac gtgaaagatc cggctaaccg cgaaaaattc
     5941 gccaaagcct ggggcgtgga aagcctgcct gctcataccg gctatcgcat cagcgagctg
     6001 ccgcaccgcg cagcgcatgg cgaagtgcgt gccgcgtaca ttatgggcga agatccgctg
     6061 caaactgatg cggagctgtc ggcagtacgt aaagcctttg aagatctgga actggtcatc
     6121 gttcaggaca tctttataac caaaaccgcg tcggcggcgg atgtcatttt gccgtcaacg
     6181 tcgtggggcg agcatgaagg cgtgtttact gcggctgacc gtggcttcca gcgtttcttc
     6241 aaagcagttg aaccgaaatg ggatctgaaa acggactggc aaatcatcag tgaaatcgcc
     6301 acccgtatgg gttatccgat gcactacaac aacacccagg agatctggga tgagttgcgt
     6361 catctgtgcc cggatttcta cggtgcgact tacgagaaaa tgggcgaact gggcttcatt
     6421 cagtggcctt gccgcgatac ttcagatgcc gatcagggga cttcttatct gtttaaagag
     6481 aagtttgata ccccgaacgg tctggcgcag ttcttcacct gcgactgggt agcgccaatc
     6541 gacaaactca ccgacgagta cccgatggta ctgtcaacgg tgcgtgaagt tggtcactac
     6601 tcttgccgtt cgatgaccgg taactgtgcg gcactggcgg cgctggctga tgaacctggc
     6661 tacgcacaaa tcaataccga agacgccaaa cgtctgggta ttgaagatga ggcattggtt
     6721 tgagtgcact cgcgtaaagg caaaattatc acccgtgcgc aggtcagcga tcgtccgaac
     6781 aaaggggcga tttacatgac ctaccagtgg tggattggtg cctgtaacga gctggttacc
     6841 gaaagcttaa gcccgattac gaaaacgccg gaatacaaat actgcgccgt gcgcgtcgag
     6901 ccgatctccg atcagcgcgc cgccgagcag tacgtgattg acgagtacaa caagttgaaa
     6961 actcgcctgc gcgaagcggc actggcgtaa tacagtcctt tctacagcct cctttcggag
     7021 gctgtttttt tatccattcg aactctttat actggttact ccctacccaa tcgtattatc
     7081 aaaatgaaaa aaattatcgc attgatgttg tttttgacat tctttgccca cgcgaacgac
     7141 tccgagcctg gcagccagta tttaaaggca gcagaggccg gggaccgacg tgcacaatat
     7201 tttcttgccg acagctggtt tagctccggc gatttgagca aagccgaata ttgggcacag
     7261 aaagccgccg acagcggtga tgctgatgcc tgcgcgctgt tggcgcagat caaaatcacc
     7321 aatccggtca gtctggacta tccacaagca aaagttcttg cagagaaagc ggcgcaagcg
     7381 ggcagtaaag aaggtgaagt aacgctggcg catattctgg taaatactca agcgggtaaa
     7441 ccggattatc caaaggcgat ttcgctgtta gaaaacgcct cggaagatct ggagaatgac
     7501 tccgccgtcg atgcccaaat gctgcttggt ttgatttacg ccaacggcgt gggcattaag
     7561 gccgacgatg acaaggcaac ctggtatttc aaacgcagct ctgcaatttc ccgaaccggt
     7621 tattccgagt actgggcggg aatgatgttc ttaaacggtg aggaaggttt tatcgagaag
     7681 aacaaacaaa aggcgctgca ctggttgaac ctgagctgta tggaagggtt tgataccggg
     7741 tgtgaagagt ttgaaaaatt aaccaacggt taaggtaggc ctgataagac gcggcaagcg
     7801 tcgcatcagg catccacaca ttgccggggg atatccccgg caatcttcaa ttattgatcc
     7861 gcagttttat caaatttgcc cagcacttca cgctcataag ccagcgcctt tttgcggtca
     7921 aatttgtgtt cccacttggc aatcaccagc accgccagcg cattacccac cacgttcagc
     7981 gcagtacgcg ccatgtcgag gatacggtca acaccagcaa taaacgccag accttccagc
     8041 ggaataccta cgctacccag cgttgccagc aacaccacaa acgacacgcc agccacgcca
     8101 gcaatccctt tcgaggtcac catcagcgtc agcaccagaa tgatttcctg ccagatggac
     8161 aggtcaatgc catacagctg agcgatgaaa atagcggcaa tactttgata cagcgtcgaa
     8221 ccatcaaggt taaaagagta accggtcggc accacgaagc tggtgatcga cgccggtgct
     8281 ccgtaggctt ccatcttctc aataattcgc ggcagtacgc tttcagagct ggcagtggag
     8341 tacgccagaa tcagctcatc tttcagaata cgaatcagga tccagacgct taacccgcac
     8401 agacgtgcca caattcccag cactaccagc gcgaagaaca gaatggcgaa atgcaccagc
     8461 agcaccagtt tcgccagtgg ccacagagac gagaaaccaa agttagccac cgtcaccgca
     8521 atcagcgcaa acacacccac cggtgcataa cgcatcacca tgtgagtcac tttaaacatg
     8581 gtttcagaga tggagcggaa cacggtcacc agcggttcac gatgcgtcgc gggcagggaa
     8641 gaaagcccca gaccaaacag caccgagaaa aagatgatcg gcagcatttc gcctttcgcc
     8701 atcgacgcca caatgttcgt cggcaccagc gacaaaatcg tgcccataat gccgtgggaa
     8761 ctgctttgta ccgcttccgt agtgctctga tatttcgaga tatcgacggt cgccagctgc
     8821 gacatatcca ccccggcacc gggctggaag acgttcgcca gagtgatccc caaaatgatg
     8881 gcgacggtgg tgatcacctc gaagtaaata atggttttcg cgccaatacg cccgagctgt
     8941 ttggcatcac caacacccgc gatacccacc accagcgtgg agatcacaat cggcacaaca
     9001 atcattttga tcagatggat gaagatatca cccgccggag agagcaaatt gacgaccagc
     9061 cagtcgcggc tgtcgctatg gtagtgcagg tagcttccca ggagaatgcc cagcaccata
     9121 gcaaacagaa tctgccaggc caggctgaat tttatatttt tcatcagaat aacttcctca
     9181 atggagcgcc gttccccgtt cagaaacgcg agaacgtgac atactgccag ggcatgaatt
     9241 gtgttgcgtt ggtttgtaga tttttttaac gcttatgatc agtatcattt gcgcggtatg
     9301 ttgcgcaacc ctttaacaac agagcttttc gctgcattaa tgcataataa cgcgagttta
     9361 tggcaggatt aatgaataac catttgttat ggaaagattt ttatttgttt aatctgcaat
     9421 aatactgatt ggtgattatt tttggcatat gttcattcga ttatttttta tgcagctcaa
     9481 tgacataatc ttcatcacca gatgattaat ccgatctccg ctgcaacagt tttgccatat
     9541 taatcgactc gaccagctgt gtccggttga tccggggtga gttgagatcc atcacttttt
     9601 gccatagttc gatggcttgc gcgtaattcg cctgcataaa cgcatcggaa gccagcagca
     9661 tcagagcggt gatttcgtta gagtccagcg cgagggcttt gtcgatcatt gcgcgagtct
     9721 gggcggtcat atgctggctg gcctggtaat aaagcaccgt cgccagcgcc gcatacagtt
     9781 cagcgttctc accacgtagt tgcaacgcct gacgatacgc cagcagcgaa ttgctgtaat
     9841 cgttttgcca cagatagtac tcgcccagta acgcccattg ttcgctgttt tgtggattag
     9901 cgcggatttt atcctgcaat gcctgaagct gcgcttccgg ggtttgctgg ctggcaaact
     9961 gatgtagcgg atcgcgctga cgctgatact ccgcacgtac cgcctgccat tttgggctta
    10021 acagataact gccgacacag aggaatacca tcaatatcgt gagtgttgtc agcattttca
    10081 ccggtatttg aggctgtttc atcgcttagc cctcactcgc cagaggatca gtgccatcag
    10141 caataacaac accactggca gcgcccacaa caccagcgtc tgacccgtta acggtgggtt
    10201 atagcgaaca aaatctccgt agcgttcggt catccagcca atgatttcga cttcgttctt
    10261 cccctccgac accatgctat aaacctgatg gcgcatactg accgccaccg gcgcattgga
    10321 ttcgagtaag ttttgattct ggcactgcgg acaacgtaac tggctggcaa tatttaacgc
    10381 ctgttgctgt tgttgcggat tggcgaattg ccaggtgtct acaacctgag cgcgcgcaaa
    10441 acaggtaaat aacagcagta gcgtgagaag ccctttattc atcgcgcttc ttcccccgcc
    10501 atccgcttaa caatgcgccc gcaatcatca acaatcctcc cccccagatc cagcgcacac
    10561 cgctttgtac atacaaacga aaagcgtaac gatccgctct ggttttttca cccatgaccg
    10621 cataccagtc atggatgccg ttccagcgaa ttgacggttc catcatttgc tgacggcgtg
    10681 cttcataaaa acgccgctcc ggcgttaatt caccaatgcg ttgctgatgg tcaaacagcg
    10741 ccactatcgc tttttcgctg gtgtaattgc ctctggcttg cagatcgagt cgctcaaaac
    10801 ggaaggtgta tcctgccagc gtcacctgct gacccggctg taaattcagg ctgatctcct
    10861 gacgactgac actggaaacc acgatccccg cggcaaataa cagcacgcca gcatgagcta
    10921 ccagcgccgg aagctgcacg cgtttgccag agacaaacgt cgccagcaca atcaccacca
    10981 gcatcaacag accaaacggt aacgtcgcgc ggttaaaata cggcgcgcca acggagaggc
    11041 gtccccagcc cagcaggccg taaatcatcg gataaagcgt tcccaccagc acgatcagta
    11101 gcactgcgca aaacagcaac agcgtagcga ggattaacat ttcccgcgat aaccccgaaa
    11161 aacgcaccgc cgggccaccg tcccgcgctc gccagccata cagagccaaa gacgcaaggc
    11221 taatcagtgc aaacaggctg aacaacggca cggcgcggac gttatccagt gcgaacgcat
    11281 gaaccgaaac cagaatgcca gaacggacaa ttaaggtgcc cagcagcgac agcatcagag
    11341 taactatcgc cagcaacagc gaccagtggc ggaaaatccc ccgctggcgt gtcagggaca
    11401 aactgtgcag cagcgcagtg gcagaaagcc agggtaataa agaggcgttt tccaccggat
    11461 cccagaacca ccagccgccc cagccgagtt cgcagtaggc ccaccaggaa ccgaggatga
    11521 tccccgccgt taatgcactc cagccaggta acgcccagcg ccagcaaatt cgggcgctgg
    11581 ccgcatcaaa ctcgccgcgc agtagactcg ccagcgccac gctcgccgct accatcaaac
    11641 cgccatagcc aaggtaaagc agcggtggat gaaagataag accgggatgt tgcagcatcg
    11701 gattgagatc gcggccttcg attgctggtg gaaatatccg cacgaaggga tcggaccaca
    11761 gcaccacaaa cagtagcagt gcggcgagca tgagagataa aacggctagc gtcagcggaa
    11821 atagcggatc gctttgctgc cgataatgcc aggcaaacag cgcgctccag gcggaaagca
    11881 gcagcaccca aagcagcagc gaaccttcat gaccgcccca caccgccgcc agtttgagtt
    11941 cccacgacag caggctgtag ctatgttggg cgacataaat gaccgagaaa tcgctgatga
    12001 gaaaacaata cgtcagtacg ccaaaggcga gcagcaggag cgcgaactgc gccagaatgc
    12061 cgatgcaagt gagtcgcatc atggcaggcc agcgcaaccg cactcccgca aaggccgtca
    12121 acggggtcaa cacgttgacc ccgagactta acaacaacgc cagaaaaccg acttcaggaa
    12181 ggaatacgtc cagaaaccac ctcttttctg gcttacgcca ccgtcaactg tccggcgtag
    12241 agaatgaaaa agcgcagcat caacacgccg accagactgg ccccacacgc cgccagcacg
    12301 gcaggaatgc cggaactgcg attgacccac ggtttgagca acattggcac aatcagcccc
    12361 agcccggcga caccaagcca gaaccaccac gtccagaaac cgccacctaa tgccgccacc
    12421 agcgcacgaa ctttaccgtc atcgcccagc gccagaccga caaaaaacgc caccagcagg
    12481 aagatttcac cccataccac ggggatttcc atccggtgaa caaagtgcgc ttccgtggaa
    12541 tgcgggttac tgcgttggcg tatcgccatg gcgatcagcg ccaccgccgc accggacgag
    12601 atgccggaga agaggagcag caccggcagg ctcgggttat tgaggaacgg atacgatttc
    12661 agcgccgaca gcagaaagcc ggtataagcc cccagcagca ccgccaacac cagcatcagc
    12721 gtttccagtc cgcgatgaac gggcgtcagc aggctcagaa ccttttgcac gatccccagc
    12781 ttcggcaacc agcgttgttg cagggcaagc aaatcatgtt caaagatttt cgccagccac
    12841 agcaccagca ccaccatgta gagctgaaac agcatcaccc ccatcgacat caccgaggta
    12901 aaactgtagt ggaacatcag cttccagaag gtccacggtc ttgtcaggtg gaagacgagg
    12961 atcaacagac cgaggatcac cgcgcccggc ccgacaatca gcgtggtgcg cagcaacgta
    13021 ctgtctgcac cgcccgcctg cgggtagaag cgacgtaaca gcacggccag cgtcaccaga
    13081 ccggcagaaa taccaatcaa aaacaggtag atggcaatcg gccagtccca caccagcgat
    13141 tcaaaatgaa atgcggaagt ctgcgtcatt ggctcacctc cccgtattta aacggtacgc
    13201 ggtacagctt cggtttggtt cccagcgcca gcttgtagcg gtaagtaggc ttctggcgca
    13261 gcagttgcga aatctcactg ttaggatcgt ccagattgcc aaacgtcagc gccttcgtcg
    13321 ggcaagcttc aacgcacgcg ggaagcttac cggcctgcaa attggtctta cgacagaaat
    13381 cgcatttgtc cgccgtcttc gtgaccggat ggataaagcg cacgcggtac ggacaggcgg
    13441 cgatgcagta ctgacaaccg acgcagagat caggattaac atcgacaatc ccgctagcgg
    13501 catcacgaaa agacgcaccg gtcgggcaga cgtcaacgca cggcgcatga tcgcagtgct
    13561 ggcaagactt acggaagaag cgatatttca catcaggaaa ttcgccctgc ggctcgctac
    13621 gaataatcgt caggcgcgag acgccttccg gcaccttgtt cacttcccga caagcatcca
    13681 tacaggcggt acagccgatg cataacgact cgtcatgcac catgccgtaa cgcaccccat
    13741 tgatattcaa cgttttcgcc accacgcgcc ctgcggtgcc gctgacggct gccagcacgc
    13801 cgacgccggt gagaaactgg cgacgagacc aggtcatggc tgctccttaa gcaacggaac
    13861 agacgccggg ttaaagttcg gattggtgcg ctgatcgctg tggcaatcga cgcaaatctt
    13921 aatccgtcct ttgtcactta acgtctgcat cgtatcttgc tgtggatgca gggaatggca
    13981 gctggcgcac gccactttgg ttacgtggac atcgtgcggc cagaacgctt tttgcaactg
    14041 ttccggcaga tgacaggaca tacagacgct gttctgctct ccgaccttat acatcggctc
    14101 gttaaagcgc atcacatctt tcaccccttc gcggtgttgc ggtgatgctt ggccgtgaca
    14161 gttggtgcag gtgaccggca atttgttatt cgggttgata acggaagcat gtttgccatg
    14221 cataccttcg gtatctggtt tatgacagtc cagacaggcg gcatccgggt tacgctgctg
    14281 ggtaacttcg tagcgatcac cagatgcctg caccgcaggg gtagcggtaa ttccgttcag
    14341 gctccacaac aggcccgacg ccagcacccc agcagttaat aacgaacgta atacgctcat
    14401 attcactcca ttgtgttttc cgccccttgc gaggcggaac ggggttattg gcttaacaga
    14461 ccgtttttac gtgcctgctc ttcccactgc gggatcaccg ttttgatgaa gtcctgcttc
    14521 tcggccttga tttgttccat gttcaggcca atggcctgct gggctttctc tttggtcgag
    14581 atatccggga tctggatttc atgggtgata cctttggtcg ccagcaggcg cgccagtttg
    14641 gtgcgcgcat ccgccgcttt atccatcgcc gtaccgagca tccgtagacc ttcttccggt
    14701 gcgtgcatat gaataccgtg ggaagcaatc gccagatccc aacgccactg ggcatgacgg
    14761 atatcgtcct gaatcggctt catttcggct tccgtcgcgc ctgcatccag cgccgctttc
    14821 gcttcgaagt gagcgtgaac cagttgatct tcaaccttga ttttcaggtc gttaatcgat
    14881 tgcttacgtt ccgcgaccac tttttgcaag gcagctttat cctgggtatg gcagttcgca
    14941 caagtctggg cgaagttatc aaacggatta ccaattttat ggtcggtgta gagtttgcct
    15001 tcggcgttct gcacttttgg catatggcag tcgatacagg tcacgttgtt tttaccgtga
    15061 atgcccgctg tccaggtttc atattccggg tgctgcgctt tcagcattgg cgttttcgac
    15121 agggagttag tccagtcaga gaaggcaatt ttgtcgtaat actgctccat attttcgact
    15181 ttcatgccgt catcccacgg gaatttaacc gctttgtttt tgccgtcgaa gtaatactcc
    15241 acatggcact gaccgcaaac catcgattgc tggtcgaaac gtccggcttt ctcaaaaggt
    15301 ttaccaatgg cttccatcgc gcgagccgca tacggacggg aaagggttaa ctccggtttg
    15361 cctttggcga actctggtga ggcggtgtta tggcagtcgg cacaacctaa gttgttgacg
    15421 atttccgggc cgccgcgcgc ccatttaccg tggaagtagc catcttcgcc gtctttctgg
    15481 atcagacgcg ccacatccgg gcttttacaa ctccagcacg ccatcggtag cggaccatct
    15541 tcagcgtttt tcggcgcacc ggtacgcagg gtttcacgca catcggtcac agcaaaggca
    15601 tgtccacgag gcttgttgta atcgcgcgag aagggatacc ccgcccacag gatcaccagc
    15661 cgtggatctt ccgccagggc gtcaacacgc tctgactgtt ccgaggtggc tttccaggag
    15721 agatattgat cgtgatgctg cggggcaaag gtttcattct tcgcttccac agttacaggt
    15781 tttgcgggag ctgccgtttg ttcagcgtga acagaagtga aaaagaaaaa aggaatcaat
    15841 aagctgaaga tacggcgtgc gtttattttt atccttgtca taggggcttc atccgaattg
    15901 cgccattgtt gcaatggcgg tttttattgt ttttcacgac agtaaccgca cctacactgt
    15961 catgacattg ctcaccccta tgtgtaacaa ataaccacac tgtgaatgtt gtctttaatc
    16021 aattgtaagt gcatgtgaaa taccacttta gagttagtca gtatcttcct ctttttcaac
    16081 agcatgcata actgcatgtt cctcaaagaa ttaatcaact tttgttggtg accttcaaaa
    16141 attaccctgc cgtttatttg caccattcta cttttgcgtg atctgtcgcc caaatactaa
    16201 acaaaactgc caatacccct acatttaacg cttatgccac atattattaa catcctacaa
    16261 ggagaacaaa agcatgagcc aaattcacaa acacaccatt cctgccaaca tcgcagaccg
    16321 ttgcctgata aaccctcagc agtacgaggc gatgtatcaa caatctatta acgtacctga
    16381 taccttctgg ggcgaacagg gaaaaattct cgactggatc aaaccgtacc agaaggtgaa
    16441 aaatacctcc tttgcccccg gtaatgtgtc cattaaatgg tacgaggacg gcacgctgaa
    16501 tctggcggca aactgccttg accgccatct gcaagaaaac ggcgatcgca ccgccatcat
    16561 ctgggaaggc gacgacgcca gccagagcaa acatatcagc tataaagagc tgcaccgcga
    16621 cgtctgccgc ttcgccaata ccctgctcga gctgggcatt aaaaaaggtg atgtggtggc
    16681 gatttatatg ccgatggtgc cggaagccgc ggttgcgatg ctggcctgcg cccgcattgg
    16741 cgcgatgcat tcggtgattt tcggcggctt ctcgccggaa gccgttgccg ggcgcattat
    16801 tgattccaac tcacgactgg tgatcacttc cgacgaaggc gtgcgcgccg ggcgtagtat
    16861 tccgctgaag aaaaacgttg atgacgcact aaaaaacccg aacgtcacca gcgtagagca
    16921 tgtggtggta ctgaagcgta ctggcggaaa catcgactgg caggaagggc gcgacctgtg
    16981 gtggcacgac ctggttgagc aagccagcga tcagcactag gcggaagaga tgaacgccga
    17041 agatccgctg tttattctct atacctccgg ttctaccgga aaaccaaaag gcgtactgca
    17101 cactaccggc ggttatctgg tgtacgcggc gctgaccttt aaatatgtct ttgattatca
    17161 tccgggtgat atctactggt gcaccgccga tgtgggctgg gttaccggac acagttattt
    17221 gctgtacggc ccgctggcct gcggcgcaac cacgctgatg tttgaaggcg taccgaactg
    17281 gccgacgcct gcccgtatgg cgcaggtggt ggacaagcat caggtcaata ttctctatac
    17341 cgcacccacg gcgatccgcg cgctgatggc ggaaggcgat aaagcgattg aaggcaccga
    17401 ccgttcgtcg ctacgcattc tcggttccgt gggcgagcca attaacccgg aagcgtggga
    17461 gtggtactgg aaaaaaatcg gcaacgagaa atgtccggtg gtcgatacct ggtggcagac
    17521 cgaaaccggc ggtttcatga tcaccccgct gcctggcgct accgagctga aagccggttc
    17581 ggcaacacgt ccgttcttcg gcgtgcaacc ggcgctggtc gataacgaag gtaacccgct
    17641 ggaaggggct accgaaggta gcctggtgat caccgactcc tggcctggtc aggcgcgtac
    17701 gctgtttggc gatcacgaac gttttgagca gacctatttt tccaccttca aaaatatgta
    17761 tttcagcggc gacggcgcgc gtcgcgatga agatggctat tactggatca ccgggcgtgt
    17821 ggacgacgtg ctgaacgtct ccggtcaccg tctggggacg gcagagatag agtcggcgct
    17881 ggtggcgcat ccgaagattg ccgaagccgc cgtagtaggt attccgcaca atattaaagg
    17941 tcaggcgatc tacgcctatg tcacgcttaa tcacggggag gaaccgtcac cagaactgta
    18001 cgcagaagtc cgcaactggg tgcgtaaaga gattggcccg ctggcgacgc cagacgtgct
    18061 gcactggacc gactccctgc ctaaaacccg ctccggcaaa attatgcgcc gtattctgcg
    18121 caaaattgcg gcgggcgata ccagcaacct gggcgatacc tcgacgcttg ccgatcctgg
    18181 cgtggtcgag aagctgcttg aagagaagca ggctatcgcg atgccatcgt aacccacaat
    18241 tgccggatgc gaatcggtaa cggtttgtag gcctgataag acgcgacagc gtcgcatcag
    18301 gcattgattg ccggatgcgg cgtaaacgcc ttatccggcc tacattcggc aagggttacc
    18361 cgagcgttaa ccttctccca taagggagcg ggaattaaaa caatccctac attacctctg
    18421 gagaatctgt gatgaatggt acgatttatc agcggataga agacaatgcg catttcaggg
    18481 agttagtcga aaaacggcaa cggtttgcca ccatcctgtc gattattatg ctggcagttt
    18541 atatcggctt tattttactg atcgccttcg cgcccggctg gctgggcacg ccgctgaatc
    18601 cgaacaccag cgtcacacgc ggtattccaa ttggtgttgg agtgattgtg atctcctttg
    18661 ttctcaccgg tatctacatc tggcgggcga acggcgaatt cgaccgtctt aataatgaag
    18721 tcctgcatga ggtacaagca tcatgaaaag agttctgacg gcgcttgccg ccacactccc
    18781 tttcgcagct aacgccgcgg atgctattag cggggccgta gagcgccagc caacgaactg
    18841 gcaggcgatt attatgttcc tgattttcgt cgtgtttacg ctcggcatta cctactgggc
    18901 atcaaaacgc gtacgttctc gtagcgacta ctacaccgca ggcggcaata tcactggctt
    18961 ccagaacggg ctggcgattg ccggggacta tatgtccgcc gcctcattct tggggatctc
    19021 cgcgctggtg tttacctccg gctatgacgg gctgatctac tcgttgggct tcctggtggg
    19081 ctggccaatc attctgtttc tgattgccga acgtctgcgt aacctgggac gctacacctc
    19141 tgccgatgtg gcctcttatc gtctgaaaca agggccgatt cgtattcttt cggcctgtgg
    19201 ttctctggta gtggtggcgc tttaccttat cgctcagatg gtaggcgcag gtaaactgat
    19261 cgagctgctg tttggcctta actatcacat tgcggtggtg ctggtcggcg tgctgatgat
    19321 gatgtacgtc ctgttcggcg gcatgctggc gaccacctgg gtacaaatta tcaaagctgt
    19381 gctgttgctg ttcggtgcca gctttatggc ctttatggtg atgaaacacg tcggctttag
    19441 cttcaacaat ctgttcagcg aagcgatggc ggtacacccg aaaggtgtcg acatcatgaa
    19501 accgggcgga ctggtgaaag atccgatctc cgcgctctct ctggggctgg gactgatgtt
    19561 tggtacggcg ggcttgccgc acattctgat gcgcttcttt acagtcagcg atgcccgcga
    19621 agcacgtaag agcgtgttct acgccaccgg atttatgggc tacttctata ttctgacctt
    19681 tattatcggc ttcggcgcga tcatgcttgt tggtgcgaat ccggaatata aagacgcggc
    19741 gggccatctg attggtggta acaacatggc ggccgttcac ctggcgaatg cagtgggcgg
    19801 caacctattc ctcggtttta tttcagcggt tgctttcgcc actatcctcg cggtggttgc
    19861 ggatctgacg ctggcgggcg catcggcggt ttcgcatgac ttgtacgcta acgtcttcaa
    19921 aaaaggcgcg accgaacgtg aagagctgcg ggtatcaaaa atcaccgtac tgatcctcgg
    19981 cgtgattgcg attatcctcg gcgtgctgtt tgagaatcag aacatcgcct ttatggtagg
    20041 ccttgcgttt gccatcgcgg cgagctgtaa cttcccgatc attctgcttt ccatgtactg
    20101 gtcgaaactg actacccgtg gcgcgatgct gggcggctgg ctggggctga ttaccgcagt
    20161 ggtgctgatg atcctcggcc cgactatttg ggtacagatc ctcggtcacg aaaaagccat
    20221 cttcccgtat gaatacccgg cgctgttctc tatcagcgtg gcattcctcg gcatctggtt
    20281 cttctcggca accgataact cagcggaagg ggcgcgcgag cgtgaactgt tccgcgcgca
    20341 gtttatccgc tcccagaccg gctttggcgt tgagcaaggc cgcgcacatt aatctctccc
    20401 cttccccggt cgcctgaccg gggaatacgc ttcctctcca acatgcatca ccttttccca
    20461 taatattaaa caaataaact cattaaaaaa tgagcgattt ttgacagtcg tagaaaatga
    20521 taatgcagag aatatgcctt ttctttcttg ttaattataa ggatatttta tgcgctataa
    20581 tggtttaaat aatatgtttt tccctctttg ccagattaac gataaccact ctatcacaag
    20641 ttcatcacat acaaagaaaa caaaatcaga taattacagc aaacatcata aaaacacgtt
    20701 aattgacaat aaagccctct ctcttttcaa aatagatgat catgaaaaag tgataggctt
    20761 gattcagaaa atgaaaagaa tttatgatag tttaccatca ggaaaaatca cgaaagaaac
    20821 ggacaggaaa atacataaat attttataga tatagcgtta tatgcaaata ataaatgtga
    20881 cgatagaatt acgagaagag tttaccttag taaagaaaag gatgtatcca ttaaggtggt
    20941 atattttata aataatgtcg ccgtccataa taatactatc gaaattccac agatagtaaa
    21001 tggtggttac gatttttcac accttagcct gaagggtatc gtgattaaag atgaagattt
    21061 atccaattcg aattttgcag gttgcagact acaaaacgct attttccagg actgtaatat
    21121 gtataaaacg aatttttatt acgccataat ggaaaaaata ctttttgata attgtattct
    21181 cgatgactca aatttcgctc agataaaaat ggccgacgga actctaaatg catgctccgc
    21241 tatgcatgtt caattctaca atgcagcaat gaatagagcc aatattaaaa ataccttcct
    21301 tgactattca aatttttata tggcgtacat ggctgaggta aatctttata aagtaatagc
    21361 gccatatgtt aatttattta aagccgacct tagtttctct aaactcgatt taattaactt
    21421 tgaacatgct gatctgtctc gcgtcaatct gaacaaagca atcctccaga atataaactt
    21481 aattgatagc aaactctttt gtacgtggct gacaaataca ttcctcgaaa tggttatatg
    21541 taccggctct aatatggcta atgttaattt taataatgcc aatttaagca actgccattt
    21601 caactgttct attttaacaa aagcctgtat gtttaatacc cgtctctatc gggttaattt
    21661 cgatgaggct agcgtccagg gaatgggcat ttccattctc cgtggggagg aaaatatccc
    21721 cattgatagt gataccctgg taacactaca gaaattcttt gaagaagatt gtacctctca
    21781 tactggcatg tcacaaactg aggataatat taatgcagtc gctatgaaga ttactgcaga
    21841 tattatgcaa cacgcagatt gaggttaagt cgaagaacag ccaatgaatt gactttacaa
    21901 gagagataac ttatttgttt tagaatcatg aatgttcgcc cgagctaacc tgttaaaggt
    21961 gcaaaagatt gcaccttttt cagattacct cgggcctggt tacgtcgctt tcggcttact
    22021 gattttcctc aattagcaac gcttcaagca aatcgagatc gtgcagtaat ttttgcagcg
    22081 tttcgttgct gatctcccgc gtggcgcgca ggtggtacag ttcagcacgt tcagaacgca
    22141 atgccgccag acggaagcga cgctcaaggt tctcttcctg cacggaactt tcaacgtcgt
    22201 tacgtccatc ggcgcgacga cgcaggttac caatgacgcg agaactgacc tccgtaagca
    22261 actggttatc gatattctct tcggtatccg ccgccagacg ctcctccatt ttctggatcg
    22321 ccacaatcgc cacttctgcc gttgccgctc gcgcaatacg ttcttctttc aattgttgcg
    22381 aatggtccgc gacttcaatg tgttgtagca gaataggcaa catcaccacg ccgacaaaca
    22441 gcgagaagag aatgacgcca gccgccagga acaccagctc atagcgcgcc gggaagacgt
    22501 taccatccgg caagagcagc gggattgaga gcacaccggc cagagtgatc gccccacgca
    22561 ccccagcgaa agacgcgatc aggatttctc gtgtggtcca cgaaccaaac tccatcggct
    22621 tcttcttcag gaagcggttg ctgaactttt tcatcgtcca cagccagccg aaacggacca
    22681 gcatcagcgc cgcatatatc agaataatat cggtaaacag catccagatt tcgacgttag
    22741 ggtcgatttc tgccgccatc agcgacgtct ccagaatacc cggcagttgc agacctaaca
    22801 gcaggaacac catgccgtta aagacaaatt ccagcatcgc ccaggtgctg tttgcgcgca
    22861 ggcgcattgc cagtggcgca cggcgcatca caccggagcg ggtgatggtc atcccggcgg
    22921 caaccgcagc gaggatgccc gaaacgccaa tatgttcggc aatcagataa gaagcgaatg
    22981 gcagcaagaa cagcagaacg atctgcgtcg cgggttcatc accgccccag cggctgagga
    23041 atcgcagcga gcgaccatac agccagctca ccacaaaacc ggcaagaata ccgccaatgg
    23101 ctactttcat aaattcgacc gtcgcaccgc cgacagtgaa gatcatcgtc cccatcgcca
    23161 ctgccacggc aaacttcaac gacaccaggc cggaagcgtc attcatcaac gcttcgccct
    23221 gcaaaatgcc catgattttt ttcgggatgc gcccttcgcc aacaatcccg gagagcgcca
    23281 cagcatcggt cggagaaagt accgccgcca gcgcaaaggc ggggatcagc ggaatgcccg
    23341 gcaccaccca gtaaataagg aagccaatgc cgaccacggt gaccaccacc agcgccagcg
    23401 cgaggccgaa aatctctcga ccatgttcga gaaattcacg ggtcggcgtt ttccagccat
    23461 cagcgaacag caacggcggg ataaacaaga ctaaaaagag ttcaggatca aactccacat
    23521 gcaaaccaaa cgtcggccac gccagtagcg caccgatggc gatttgcata agcggaagcg
    23581 ggatctgaaa gggcatgaca cgagtgacca ccccggacag cgagaccacg agggtcatta
    23641 tcagtatggt gaagaagatt tccatgcgtt ccctgtttgc gatgtttttt atgatctgag
    23701 aaagacgtta tgcctgaatt ctcatttttt cagaatatcg caaaagctca cacaatgtgc
    23761 ggagaaaata aaaacgggca gccattgcca cccgttttca gaatccgatg aaagattaga
    23821 tagcccagcc acccgcatag aaggtcacca gcgccacggc gataacgacc gtaccgatgt
    23881 tcaacttgcg ccattcaccg gaaaccagac gaccaatcac cagagtcgcg aagccgatca
    23941 tgatgcctgt tacgatgtta caggtcagca cgatgaatac cgccgtaacc agacccgcca
    24001 tcgcatcaac aaaatcagca aagtcgattt tcgccacgtt gctcagcatc agcaggccaa
    24061 cgtacatcag cgccggagcc gttgcatacc ccggaacgag gtaagaaagc ggagagagga
    24121 acagaatcag caggaacagc acgccaacgg tgatagccgt caacccggtt ttaccgcccg
    24181 ccgccgtacc cgccgcagac tcgatgtata ccgctgccgg agctgcaccc accaggccag
    24241 agaaaacgct gctcatggag tcagtggtca gtgctttccc gccgtcgatg atctgcccat
    24301 ctttatccag caggttcgcc tggccggcga cagcgcggat agttccggtg gcatcaaata
    24361 ctgccgtcat caccagcgcc agaacgcttg gcaggactac aggattcagc gcgcccataa
    24421 tgtccaggct gccaatcagt gaattgccgt tttcatcgct caatgaaggc atggcgaaaa
    24481 cgccggagaa atggacgtta ggatcgaaga tcaaaccgac aattgagata ccgataatgg
    24541 tcagcagaat gccaccaggg actttcagtt tttccaagcc gatgatcacc gccagacctg
    24601 ccagtgacat aatcaccggg aaggtcgcga aatcacccag cgcaacgggc agaccatcaa
    24661 gcgggttttt aatcaccaga ccgacaccat tagcggcaat gagcagcagg aacagaccga
    24721 taccgatccc cgtgccgtgc gccacaccgt gcggcaagtt gcgcaaaatc cagctacgga
    24781 tacccgtggc agaaattacc gtaaacagca cacccatcag gaacacggca cccagcgcga
    24841 caggtacgct aatatgttgc cccagcacca ggctgaatgc ggtaaacgcc gtcagggaga
    24901 tggcgcaacc aatcgccaac ggcagattag cccacagacc catcacgata gaaccgagtc
    24961 cggcaaccag acaggttgca acgaaaactg ccgcaggcgg gaagcctgct ttacccaaca
    25021 tacctggaac gacgatgacc gagtagacca tcgccagaaa cgttgttaac ccggcaacca
    25081 cttcctgacg gacagtgctt ccacgttgtg aaattttaaa ccaggcgtcg agtgaaccgc
    25141 cggtacgcgc tgatggcgta gacataagaa aacatcccct gagagtttaa ttttcgtcaa
    25201 gatgcgtggc ggacaatccc ctgccagcga aacgttatct tcctgctcca ggtttgcggc
    25261 aatttggtgc gtcgcaaaaa agcaaacgtt tagctccgcg catacaaaac gggtggtaaa
    25321 aaaaagcaaa cgattatccg gcgatcatag cggctttttc aaccgaagtt tgctgctttt
    25381 ttctctttta ttgtcttgac gatgaaaaac caggcaattg atgcgcaaac gttatcgtcg
    25441 atgtgcaaga ttgcagcgtt atcgagccgt ataccgttct ggattgcgat aaaaaatcgt
    25501 ctgagatgac aaaaatattg cggctaaaaa gtatactttt acactggcga agaaacgtcg
    25561 gtgacataca attaaagcat caacaccaac cggaacctcc accacgtgct cgaatgaggt
    25621 gtgttgacgt cgggggaaac cctcctgtgt tccagcggga tagagagaaa gacaaagacc
    25681 ggaaaacaaa ctaaagcgcc cttgtggcgc tttagttttg tgtttgcgga taattccaaa
    25741 agagctcgat cagtcccctc gccccttcgg ggagagggtt agggtgaggg gaacaggcag
    25801 caccagtgca aatatttacc ctcaccccgg ccccatagtt aacgatgagt cagcttgtcc
    25861 ttgtggcgct ttagttttgt tcatcttcca gcaagcgtgc gccggtacct tcttctccta
    25921 agcggtcgcc cgggttacgc aacgggcaat cactgcgcga aaggcagcca caaccaatac
    25981 atccgtccag ttcgtcacgc agcgccacta aggtatgaat gcgccgatcc aactcttctc
    26041 gccattggga cgaaagctgt ttccactctt tcgcacttaa cgtatgccct tctggcaaca
    26101 cgccaaacgc ttcaccaatg gtcgccagcg gaatgccaat acgctgagcg attttgataa
    26161 ttgcgacata tcgcaacaca tcacgtttat atcgccgctg attgccgctg ttacggatac
    26221 tagtaatcaa ccctttgctt tcatagaaat gcagcgccga taccgccaca ccgctgcgtt
    26281 tcgccacttc gccaggggtt agcagcgctt taatgcgggg taatttcttt tccataaatc
    26341 gctttacctc aagttaactt gaggaattat actccccaac agatgaatta acgaactgaa
    26401 cactgaaaag aggcagattt atgtcccatc agaaaattat tcaggatctt atcgcatgga
    26461 ttgacgtgca tattgaccag ccgcttaaca ttgatgtcgt cgcaaaaaaa tcaggctatt
    26521 caaagtggta cttgcaacgg atgttccgca cggtgacgca tcagacgctt ggcgattaca
    26581 ttcgccaacg ccgcctgtta ctggccgccg ttgagttgcg caccaccgag cgtccgattt
    26641 ttgatatcgc aatggacctg ggttatgtct cgcagcagac cttctcccgc gttttccgtc
    26701 ggcagtttga tcgcactccc agcgattatc gccaccgcct gtagttttat tgcccgcgcg
    26761 ttaactcccg cgcgggtaat tgctccatcc attgcataaa cacctgcggc ggcatcgcct
    26821 tcgcaaaaaa ccatccctgg caatagcgca cgccgcgttt gcgcagccag ttaacctgct
    26881 cctcagtttc gacgccttca gcgatcgttt ttaaccccag gctgtgcgcc agctcgatga
    26941 tgtgttccgc aatcaaatga ctggttttgt gggtggtcag cgtttcaaca aacgatttat
    27001 cgattttcaa aatatcgaca ttcaatgatt taaggttatg caagttagag tagccaatac
    27061 caaaatcatc aattgccact tcgtaaccag cctggcggaa agccagaatt aatcggcgtc
    27121 attttttcga catcaagaaa tgcatgctca gtcacttcaa atttaatttg ctgcggacgc
    27181 accgcgtatt gctctgtttt ctgattgatt cgcgctatca accgtgacgt atgaaaatcg
    27241 gaggccgaca ggttaataga aacatagcga tcggcatgtg ttgccaggta cgcgcccaga
    27301 tcgcggaaga cattatcaat aacataatca gttatctgtt ctatcatccc ctccttttct
    27361 gccagcggaa taaactctgc cggattcatt atttgcccct gctcaccagg ccaacgtaac
    27421 aacgcttcag cgccgataca tttttctgtt ttgatatcga ttattggctg gtaataaaga
    27481 caaagttgat gtttttcgag ggcgcgttgc aatttacgtt tgggagataa atagttttgt
    27541 cgaatacgta gccagagtaa tagcagaaca agactcccca aaataccggc gggcaacgca
    27601 aaaataagat gattataaaa atgggttata agacgttgat atgaagtcgc aacaatggct
    27661 gcaattgggc gttttgtcga ataaattgtc gcatataaat agccatttct ttgtacggtt
    27721 aaatccttca aatgaatcag cggagaaaac gttgctgccg aggcctcttt gcttaacgag
    27781 aaaaaggttt tcatcaccgt atcatacaca ccccattgca atgtcgggtc atcagacatc
    27841 acttcactcc agaagagagg gttgataacc gccacataat ttccccgctg catataggtc
    27901 attttatagc cagagaaaaa aggcgtatcg cggtaataat agatagaaac gttaggttca
    27961 cgcttataat cggccggtgc aatcgtatag ccgtttacag gcgctatcag cgatgagcat
    28021 aaaaaatggt tatcacgaat tcattaatat aaagatagcc acgaataata ttcaacattc
    28081 gcttttgatg ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta
    28141 aatctgcctg gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga
    28201 gctgattatt cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg
    28261 gaaagagaac taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac
    28321 tcattgctat caatttcctg cctgtttata cccggccatt gcaaggtgaa accctggcta
    28381 ccgacgtctt tagataaatc ttacggtata aatagcactt ttgcctgatt tgaaattgat
    28441 acctttttaa tcgtcacctg gatgaaaata attcattgat attcaaaatt aattttctac
    28501 aggaatacag aggcggcggg aagatatgcc cttaatgcca ctgaaaaacc acataatttg
    28561 tacagcaata aaagtcacgg cctaatatgg ctaacaaaca atcaattgtg atatagttca
    28621 caaaattaat gaaacaaaca gagtgtttca tttttgtgtt ctgcaagccc cgcgatagcc
    28681 agagtgtacc acgcctcccg tgaacaacgc cgcgctgtcc agggcatcgg ctcttttcag
    28741 aggaattgtt tgatggctac cctcaccact ggcgtggttc ttcttcgctg gcaacttctt
    28801 agtgccgtaa tgatgtttct ggccagcaca ctcaacatcc gttttcgtcg gtctgattat
    28861 gtcgggcttg cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg
    28921 gggttgcttg gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg
    28981 atgatagaag agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta
    29041 gaa
//
LOCUS       DAJTQQ010000045        29003 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000045 DAJTQQ010000000
VERSION     DAJTQQ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29003
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.045"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..203)
                     /locus_tag="OO309_002167"
     CDS             complement(<1..203)
                     /locus_tag="OO309_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR5847302.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALK"
     gene            513..2285
                     /gene="aspS"
                     /locus_tag="OO309_002168"
     CDS             513..2285
                     /gene="aspS"
                     /locus_tag="OO309_002168"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR5847303.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2403..2855
                     /gene="nudB"
                     /locus_tag="OO309_002169"
     CDS             2403..2855
                     /gene="nudB"
                     /locus_tag="OO309_002169"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR5847304.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2884..3624
                     /locus_tag="OO309_002170"
     CDS             2884..3624
                     /locus_tag="OO309_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5847305.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3659..4180
                     /gene="ruvC"
                     /locus_tag="OO309_002171"
     CDS             3659..4180
                     /gene="ruvC"
                     /locus_tag="OO309_002171"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR5847306.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4182..4784)
                     /locus_tag="OO309_002172"
     CDS             complement(4182..4784)
                     /locus_tag="OO309_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR5847307.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4854..4919
                     /gene="yobI"
                     /locus_tag="OO309_002173"
     CDS             4854..4919
                     /gene="yobI"
                     /locus_tag="OO309_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR5847308.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5058..5669
                     /gene="ruvA"
                     /locus_tag="OO309_002174"
     CDS             5058..5669
                     /gene="ruvA"
                     /locus_tag="OO309_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR5847309.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5678..6688
                     /gene="ruvB"
                     /locus_tag="OO309_002175"
     CDS             5678..6688
                     /gene="ruvB"
                     /locus_tag="OO309_002175"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR5847310.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6835..7620)
                     /gene="znuB"
                     /locus_tag="OO309_002176"
     CDS             complement(6835..7620)
                     /gene="znuB"
                     /locus_tag="OO309_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR5847311.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7617..8372)
                     /gene="znuC"
                     /locus_tag="OO309_002177"
     CDS             complement(7617..8372)
                     /gene="znuC"
                     /locus_tag="OO309_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR5847312.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8451..9383
                     /gene="znuA"
                     /locus_tag="OO309_002178"
     CDS             8451..9383
                     /gene="znuA"
                     /locus_tag="OO309_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR5847313.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9399..10721
                     /gene="mepM"
                     /locus_tag="OO309_002179"
     CDS             9399..10721
                     /gene="mepM"
                     /locus_tag="OO309_002179"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR5847314.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10841..11812
                     /gene="lpxM"
                     /locus_tag="OO309_002180"
                     /gene_synonym="msbB"
     CDS             10841..11812
                     /gene="lpxM"
                     /locus_tag="OO309_002180"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5847315.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11943..13385)
                     /gene="pyk"
                     /locus_tag="OO309_002181"
     CDS             complement(11943..13385)
                     /gene="pyk"
                     /locus_tag="OO309_002181"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR5847316.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13513..14382)
                     /gene="hexR"
                     /locus_tag="OO309_002182"
                     /gene_synonym="yebK"
     CDS             complement(13513..14382)
                     /gene="hexR"
                     /locus_tag="OO309_002182"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR5847317.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14719..16194
                     /gene="zwf"
                     /locus_tag="OO309_002183"
     CDS             14719..16194
                     /gene="zwf"
                     /locus_tag="OO309_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR5847318.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16429..18240
                     /gene="edd"
                     /locus_tag="OO309_002184"
     CDS             16429..18240
                     /gene="edd"
                     /locus_tag="OO309_002184"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR5847319.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            18277..18918
                     /gene="kdgA"
                     /locus_tag="OO309_002185"
                     /gene_synonym="eda"
     CDS             18277..18918
                     /gene="kdgA"
                     /locus_tag="OO309_002185"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR5847320.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(18974..20152)
                     /gene="purT"
                     /locus_tag="OO309_002186"
     CDS             complement(18974..20152)
                     /gene="purT"
                     /locus_tag="OO309_002186"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR5847321.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            20286..20576
                     /gene="yebG"
                     /locus_tag="OO309_002187"
                     /pseudo
     CDS             20286..20576
                     /gene="yebG"
                     /locus_tag="OO309_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            20643..20999
                     /locus_tag="OO309_002188"
     CDS             20643..20999
                     /locus_tag="OO309_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR5847322.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            21326..21985
                     /locus_tag="OO309_002189"
     CDS             21326..21985
                     /locus_tag="OO309_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR5847323.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            22195..24255
                     /gene="ptrB"
                     /locus_tag="OO309_002190"
     CDS             22195..24255
                     /gene="ptrB"
                     /locus_tag="OO309_002190"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR5847324.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(24252..24914)
                     /gene="exoX"
                     /locus_tag="OO309_002191"
     CDS             complement(24252..24914)
                     /gene="exoX"
                     /locus_tag="OO309_002191"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR5847325.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(24938..25594)
                     /locus_tag="OO309_002192"
                     /pseudo
     CDS             complement(24938..25594)
                     /locus_tag="OO309_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(25696..25926)
                     /gene="holE"
                     /locus_tag="OO309_002193"
     CDS             complement(25696..25926)
                     /gene="holE"
                     /locus_tag="OO309_002193"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR5847326.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            26065..26439
                     /gene="yobA"
                     /locus_tag="OO309_002194"
     CDS             26065..26439
                     /gene="yobA"
                     /locus_tag="OO309_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR5847327.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            26443..27315
                     /gene="copD"
                     /locus_tag="OO309_002195"
                     /pseudo
     CDS             26443..27315
                     /gene="copD"
                     /locus_tag="OO309_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            27328..27669
                     /locus_tag="OO309_002196"
     CDS             27328..27669
                     /locus_tag="OO309_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR5847328.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(28062..29003)
                     /locus_tag="OO309_002197"
                     /pseudo
     CDS             complement(28062..29003)
                     /locus_tag="OO309_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         7337 a         7131 c         7473 g         7062 t
ORIGIN      
        1 tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa cacgggctga
       61 ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc atcggcagta
      121 tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac caacgcagtg
      181 gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg cctcacacct
      241 taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag ttcgggcaac
      301 acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt ggtaacgagg
      361 cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa tagccgcctt
      421 ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg cagcaggggg
      481 catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt ggacagctcc
      541 gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt cgtcgtgatc
      601 ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag gtatttttcg
      661 atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag ttctgcattc
      721 aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat atggcgaccg
      781 gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat gttctgccgc
      841 ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac ctcgacctgc
      901 gtcgcccgga aatggcgcaa cgcctgaaaa cccgcgctaa aatcaccagc ctggtgcgcc
      961 gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc aaagccacgc
     1021 cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa ttctacgcac
     1081 tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt gaccgttact
     1141 atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag cctgaattta
     1201 ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa gtgatggaag
     1261 cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat ttcccggtaa
     1321 tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg cgtaacccga
     1381 tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta tttgcggggc
     1441 cagcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc gcatcgctga
     1501 cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg aaaggtctgg
     1561 cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc ccggtagcga
     1621 agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg caagatggcg
     1681 atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg ggcgcactgc
     1741 tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca ccgctgtggg
     1801 ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca atgcaccatc
     1861 cgttcacctc accgaaagat atgactgctg cagaactgaa agctgcaccg gaaaatgcgg
     1921 tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt tcagtacgta
     1981 tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac gaagaggaac
     2041 agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg ccgcacgcag
     2101 gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac aatatccgtg
     2161 acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa gcaccgagct
     2221 ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag gctgagaata
     2281 actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca actgagtgaa
     2341 ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa cgaagaggca
     2401 gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc tatgcacaag
     2461 atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg cagtcggtaa
     2521 ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa gtaaaggaag
     2581 aggttaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag cgcacggtag
     2641 agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg cgtaatacgg
     2701 aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact gaacatctgg
     2761 cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg agcaaccggc
     2821 aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc tggagatatt
     2881 tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc gcaggatgct
     2941 aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc taagctgggc
     3001 ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc actgtctaac
     3061 aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg tgatgatgat
     3121 gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc aatcatgatt
     3181 gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc attcagcaaa
     3241 tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa aaaaggcgtg
     3301 atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga agcaggtgct
     3361 gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg ggaagaaatg
     3421 ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc ggaagtttcc
     3481 atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact gatgcgtctg
     3541 atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg tgaaatctct
     3601 gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga gacgcgtgat
     3661 ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg tcatccgcca
     3721 ggtaggtagg caactgtcct acctgggtag cggatgcata cgcaccaaag tggatgattt
     3781 accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc agttccagcc
     3841 tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag ccctgaaact
     3901 gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc cggtatttga
     3961 atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg aaaaaagcca
     4021 ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg cggatgccgc
     4081 cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga tgcagatgag
     4141 cgaatcgcgg ctgaacctga cgagagggcg actgcgttaa gttataccgc ctcggtcagt
     4201 tccggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg atgatgccac
     4261 aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa ccagaatttc
     4321 cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt ctctatttca
     4381 cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttga acagaactgg
     4441 cgcgaagatt cgtatttaaa acccgtgtgg taaagtttgc gtagccggga gggaacctgt
     4501 tcaacaattc gttgtttttg ttgttctgtc agcccagcgt ctaatcgctt tatagcatag
     4561 cgttgattag acgaacgttt aataaaacga gatagcgtgg tgatggttga aaggggaacc
     4621 cggctttctg caaccaggaa gtcttcaccg ttatgaccga taatgatccc gacgtgatta
     4681 ctccagcaat ttgatgcagc tgatatttga ccaaataagg cagcacctat acatgtaaag
     4741 acgatatctc caatttcata ttcagcaggg taattaatat tcacgttgtc attccattga
     4801 aatagataca aatatacgta atagcataaa aaaattattt ttcaggagtc gcaatgtata
     4861 tttttattac gcatttcttc actgaatatg taatattcaa atatttgctt ccaatataac
     4921 ctgttgaata aattatactg tgccattttt cagatcatcg ggacacctcg caagttttct
     4981 tcatccttcg ctggatatct atccagcatt tttttatcat acagcattat ctttgattca
     5041 ttacgcagga gcgtcatgtg ataggcagac tcagaggcat catcattgaa aaacaacccc
     5101 cgctggtgtt aattgaagtg ggcggcgtag gctatgaagt gcatatgccg atgacctgtt
     5161 tttatgaact ccctgaagcg ggtcaggaag cgatcgtttt cacccacttt gtggtgcgtg
     5221 aagacgcgca actgctgtac ggttttaaca ataaacaaga acgcacgttg ttcaaagagt
     5281 tgatcaaaac caacggcgtc ggcccgaagt tggccctggc gatcctctcc ggaatgtcag
     5341 cgcagcagtt cgttaatgcc gttgagcgtg aagaagtggg ggcactggtg aaactgccgg
     5401 gtattggcaa aaaaaccgcc gaacgcttga ttgttgaaat gaaagaccga tttaaaggtt
     5461 tgcatggcga tctctttacg ccagccgccg acctggtact cacgtctcct gctagcccgg
     5521 cgaccgacga tgctgaacaa gaagcagttg ccgcgctggt ggcgctaggc tataaaccac
     5581 aagaagcaag ccgcatggtg agcaaaatcg ctcgccctga caccagcagt gaaacattaa
     5641 ttcgcgaagc cctacgcgcc gcgttatgag gtaaaggatg attgaagcag accgtctgat
     5701 ttctgccggt accactttgc cggaagatgt agcagatcgc gccattcgcc ctaaattact
     5761 ggaagagtat gttggtcagc cgcaggttcg ttcacagatg gagattttca tcaaagcagc
     5821 gaaactgcgc ggcgatgccc tcgatcattt gttgattttt ggtcctccgg ggttgggtaa
     5881 aactacgctt gccaacattg tcgccaatga aatgggcgtt aatttacgca cgacttctgg
     5941 tccggtgctg gaaaaggcgg gcgatttggc tgcgatgctc actaaccttg aaccgcatga
     6001 cgtgctgttt attgatgaga tccaccgttt atcgccagtt gttgaagaag tgctgtaccc
     6061 ggcaatggaa gactaccaac tggatatcat gattggtgaa ggtccggcgg cacgctccat
     6121 taaaattgat ttgccgccgt ttaccctgat tggcgcaacc acgcgcgcag gttcgctgac
     6181 atcaccgttg cgcgaccgtt ttggtattgt gcaacgtctg gagttttatc aggtgccgga
     6241 tctgcaatat atcgtcagtc gcagtgcacg ctttatgggg cttgagatga gtgatgacgg
     6301 tgcgcaggaa gtagctcgtc gcgctcgcgg tacgccacgt attgccaacc gtctgctgcg
     6361 tcgagtgcgt gatttcgccg aagtgaagca cgatggcacc atctcggcag atatcgctgc
     6421 tcaggcgctg gatatgttga atgtcgatgc tgaaggtttc gattatatgg accgcaaatt
     6481 gttgctggcg gtaatcgata agttctttgg tgggccggta ggtctggata acctggcggc
     6541 agccattggc gaagaacgtg aaaccattga agatgtactg gaaccttatt tgattcagca
     6601 aggctttttg cagcgtacac cgcgcgggcg tatggcgacg acgcgggcgt ggaatcactt
     6661 tggcataacg ccgccagaaa tgccgtaagt cggattggtc gctcgcgccg catccgacac
     6721 tggcagtgtg tctgatgcga cactgttcgc gtcttatcag gccagccaga gatagcgaac
     6781 cgtaggtcgg ataaggcgct cgcgccgcat ccgacaaatg tgttcagcga tagattagct
     6841 ggcctgcttt ttcatcatac tgataataaa taacagtgcc gcacatagca ccaccgacgg
     6901 acccgccggc gtatcgtaaa acgcggaaaa ggttaaaccg ccagttactg ccaccatccc
     6961 caccaaaaca gcgacaccag ccatctgttc cggcgtacgg gcaaagcgac gcgcagtagc
     7021 tgcaggaata atcagcaacg aagtaataat caacgcaccg acaaatttca tcgctacacc
     7081 aatcgtcaat gccgtcacca gcatcaacaa caatttcact tgctgtaatt tcacaccatc
     7141 aacaaacgcc agatccgggc taatcgtcat cgacagcaaa ttgcgccatt gccagaacaa
     7201 aatagccacc acgatgacca cgccaatcgc aatagagatg agatcttctg gcgtcactgc
     7261 cagtaaatca ccgaacaggt aagccatcaa atcaacacga atgttagaca tcagactaac
     7321 gaccactagg cccagcgaca gggcactgtg cgccataatc cctaataacg tgtcgatcgc
     7381 cagctgtgga cgcttctcca gccataccag accgcccgcc agcagcagcg taacggcaat
     7441 caccgcatag aatggattca cgtccagcaa caaaccaaac gcgacaccaa gtaatgaggc
     7501 atgagccagc gtatcaccga aataagacat acgacgccag actacaaacg aacccagcgg
     7561 acccgcggca caggcgagca tgatcccggc taaccaaccg ggaaataata attcaatcat
     7621 gagcgatcat ttccccgacg caaaacaatt cgtccctgta aatcgtgacg atgattatga
     7681 tgatggcgat agatacccag ttgttcagca ccacgaggac caaacattga aataaactcc
     7741 ggatgcaggg aaacaacttc cggtgtgccg gaacaacaaa tgtggtgatt caggcacagc
     7801 acttcatcgg tttttgccat caccagatgc agatcgtgag aaaccattaa aacgccacaa
     7861 tccagttcac gacgcaactg gtcaataagg tcatataacg ccacttggcc atttacatcc
     7921 acgccttgag tgggttcatc cagcactaat aattgcgggc aatttaacaa tgctcgcgct
     7981 aatagtacac gctgcgtttc gccaccagag agcttttgca tcggtgcgtt aatcaggtgc
     8041 ccggcctgga cacgtttcag tgcaggcaaa atatcttctt tatgtgtacc agggcgtaag
     8101 cgtaaaaaac ggtttacggt cagtggcaac gtggtgtcga gatacagctt ctgcggtaca
     8161 tagccgatgc gcagttttcc gttgcgcttg ataacccctt catcgggtgt taccagcccg
     8221 agcactaccc gtaccagtgt cgacttacct gcgccatttg gcccaagtaa agtcaaaatt
     8281 tttccaggtt taagttccag cgacacatca gagaggacgc ggcgttggcc aaaagaaacc
     8341 gagacatttt ccagggaaac cagacttgtc atgttaattt tagtcttgca gtagtcatga
     8401 aatgttataa tatcacactt ctcatattca ttacgatgat tggtcgcatt atgttacata
     8461 aaaaaacgct tcttttcgca gcattatccg ccgctctctg gggaggtgca acacaggccg
     8521 cagatgccgc cgttgtcgct tcgcttaaac ccgttgggtt catcgcttct gccattgctg
     8581 atggtgtaac agaaacggag gttttactgc ctgacggcgc ttcagaacat gattattcac
     8641 tgcgtccatc ggatgtaaaa cgcttacaga acgcggactt agtcgtttgg gttggcccgg
     8701 agatggaagc gtttatgcaa aaaccggtaa gcaaattacc agaagcgaag caggtaacga
     8761 ttgcgcagct tgaagatgtg aaaccgctac tgatgaaaag tattcacggc gatgatgatg
     8821 atcacgacca cgcggaaaaa agtgacgaag atcaccatca cggcgatttc aacatgcatc
     8881 tttggctttc cccagagata gcgcgggcta cagcggttgc aatccatgga aaattagtgg
     8941 aacttatgcc gcaaagtcga gccaaacttg acgccaacct gaaggatttt gaggcacaat
     9001 tggcctcaac cgaaacgcag gttggtaacg agctcgcgcc gctcaagggg aaaggttatt
     9061 tcgtttttca cgatgcttac ggctatttcg aaaaacagtt cggactgaca ccgcttggtc
     9121 attttaccgt taaccctgag attcaacctg gcgcgcagcg tttacatgaa ataagaacac
     9181 agttggttga gcaaaaagca acctgcgttt ttgctgagcc acagttcagg ccagcggtcg
     9241 ttgagagcgt cgcacgaggg acatccgttc gtatgggaac gttggatccc ctggggacga
     9301 atatcaaact gggtaaaaca agctattcag aattcctgag tcaattagcc aaccagtatg
     9361 cgagctgcct gaaaggagat taatgaggaa gtgattacgt gcaacagata gcccgctctg
     9421 tcgccctggc gtttaataat ttaccgcgac cacaccgcgt tatgttgggg tcgctcaccg
     9481 ttcttactct ggccgtcgct gtctggcggc cctatgttta tcaccgtgat gccacgccaa
     9541 ttgtcaaaac cattgagctg gaacagaacg aaattcgttc gctcttacct gaagccagtg
     9601 agccgattga tcaagctgca caagaagatg aagccattcc ccaggacgaa ctggatgaca
     9661 aaatcgccgg tgaagcgggc gtgcatgaat atgttgtttc cactggcgat acgctaagca
     9721 gcattctcaa tcagtatggt attgatatgg gtgatatcac ccaacttgct gctgccgaca
     9781 aagaattgcg taacctgaaa atcggtcaac aactctcctg gacattaacc gcggacggcg
     9841 aactgcagcg cctcacctgg gaagtgtctc gtcgtgaaac ccgaacctat gaccgtactg
     9901 ccgctaacgg ttttaaaatg accagcgaaa tgcagcaagg agagtgggtt aacaatctgc
     9961 tgaaaggtac cgtcggaggg agctttgttg ccagcgccag aaacgccggt ttaaccagcg
    10021 ccgaagtgag cgcagtgatt aaagccatgc agtggcaaat ggatttccgc aaactgaaaa
    10081 aaggcgatga atttgcggta ttaatgtctc gagaaatgct tgatggtaaa cgtgagcaaa
    10141 gccagctgct gggcgtacgt ttgcgttcag aaggtaaaga ttattacgca atccgcgctg
    10201 aggatggcaa attctacgat cgtaacggta ctggtctggc gaaaggattc ttgcgattcc
    10261 cgacggcgaa acagttccgt atctcgtcta actttaaccc gcgtcgaact aatccggtga
    10321 caggtcgcgt tgcaccacac agaggtgttg atttcgccat gccacaaggt acgccagtgc
    10381 tttcagtggg tgacggtgaa gtggtggttg ccaaacgcag tggcgcagca ggttattatg
    10441 tggctattcg tcatggtcgc agctacacca cgcgttatat gcacttgcgc aagattctgg
    10501 tgaaaccggg acagaaggtg aaacgtggcg accgtatcgc gctttccggt aataccggac
    10561 gttcaactgg gccgcatctg cactatgaag tatggataaa ccagcaggcc gtaaacccgc
    10621 tgacggcaaa actgccgcgt accgaagggc tgaccggctc cgatcgtcgc gaattcctgg
    10681 cgcaggccaa agagattgtg ccgcagctac ggtttgatta attaacatcc attcgcagcc
    10741 ggtacgcagt cagtaccggc tttttttatt tggtgcgggg caagttgtgc cgctacacta
    10801 tcaccagatt gatttttgcc ttatccgaaa ctggaaaagc atggaaacga aaaaaaataa
    10861 tagcgaatac attcctgagt ttgataaatc ctttcgccac ccgcgctact ggggagcatg
    10921 gctgggtgta gcagcgatgg cgggtatcgc tttaacgccg ccaaagttcc gtgatcccat
    10981 tctggcacgg ctgggacgtt ttgccggacg actgggaaaa agctcacgcc gtcgtgcgtt
    11041 aatcaatctg tcgctctgct ttccagaacg tagtgaagct gaacgcgaag cgattgttga
    11101 tgagatgttt gccaccgcgc cgcaagcgat ggcaatgatg gctgagttgg caatacgcgg
    11161 gccggagaaa attcagccgc gcgttggctg gcaagggctg gagatcatcg aagagatgcg
    11221 gcgtaataac gagaaagtta tctttctggt gccgcacggt tgggccgtcg atattcctgc
    11281 catgctgatg gcctcgcaag ggcagaaaat ggcagcgatg ttccataatc agggcaaccc
    11341 ggtttttgat tatgtctgga acacggtgcg tcgtcgcttt ggcggtcgtc tgcatgcgag
    11401 aaatgacggt attaaaccat tcatccagtc ggtacgtcag gggtactggg gatattattt
    11461 acccgatcag gatcatggcc cagagcacag cgaatttgtg gatttctttg ccacctataa
    11521 agcgacgttg cccgcgattg gtcgtttgat gaaagtatgc cgtgcgcgcg ttgtaccgct
    11581 gtttccgatt tatgatggca agacgcatcg tctgacgatt caggtgcgcc caccgatgga
    11641 tgatctgtta gaggcggatg atcatacgat tgagcggcgg atgaatgaag aagtcgagat
    11701 ttttgttggt ccgcgaccag aacaatacac ctggatacta aaattgctga aaactcgcaa
    11761 accgggcgaa atccagccgt ataagcgcaa agatctttat cccatcaaat aaaaaagcct
    11821 ctcgcgagga gaggccttcg cctgatgata agttcaagtt tgcttcagaa tattcgaaat
    11881 ctgttgaact atcattgaac tataggccgg atgtggcgtt ttcgccgcat ccggcaacgt
    11941 acttactcta ccgttaaaat acgcgtggta ttagtagaac ccacggtact catcacgtcg
    12001 ccttgggtga caatcaccag gtcaccagac atcaagtaac ctttatcgcg cagcagatta
    12061 accgcttcgc tggcagctgc tacgccgtca ttagcgctat caaagggcac cggcgtaacg
    12121 ccacgataga gagcagtcag gttcagcgta cgttcatggc gcgacatggc gaaaattggt
    12181 agaccagagc tgatacggga ggtcatcagc gcggtacgac ccgattcggt catggtaatg
    12241 atcgccgtaa cgcccttcag gtggttagct gcgtacattg ccgacatggc aatagcttct
    12301 tccacattgt cgaactgaac gtccagacgg tgtttagaaa cgttgatgct cgggattttt
    12361 tccgcaccca ggcaaacgcg cgccatggct gcaacagttt ctgacggata ctgcccagcg
    12421 gcagtttctg cagacagcat cacagcgtca gtaccatcca gaacggcgtt tgctacgtcc
    12481 atgacttctg cacgcgtcgg catcgggtta gtaatcattg actccatcat ctgggtcgcc
    12541 gtgattaccg ctcggtttag ctgacgcgca cgacggatca acgctttctg aatgccgacc
    12601 agttccgggt cgccaatttc cacaccgagg tcgccacgtg caaccattac cacgtcagag
    12661 gcgaggatga tgtcatccat tgcatcctgg ctgcaaacgg cttccgcacg ttcaaccttg
    12721 gcaacaattt tcgcatcaca tcctgcatcg cgtgccaggc gacgggcata gttcagatct
    12781 tcgccacagc gtgggaagga gacagccagg taatctacgc caatcaacgc cgcagtctta
    12841 atgtctgctt tgtctttttc ggtcagcgct tcagccgaca aaccgccgcc aagtttgttg
    12901 atacctttat tgttggagag gggaccaccg acggtgactt cggtgaacac tttcatgccc
    12961 tgaacttcca gtacttttaa ctggacgcga ccatcgtcca gcagcaggat gtcaccaggc
    13021 acgacgtcag taggcaggcc tttgtagtcg ataccgactt tttctttgtc gccttcacct
    13081 ttacccaggt tggcgtcgag caggaattta tccccaatat tgaggaaaac tttgccttct
    13141 ttaaaggtgg atacacggat tttgggcccc tggaggtcac ccagaatagc cacatgacgc
    13201 cccagttttg cggcaatctc acgaacttta tccgcgcgca ttttgtgatc ttcaggcgag
    13261 ccgtgagaaa agttcatacg tacaacgttg gcacccgccg cgataacttt ttcaagatta
    13321 ttatcgcgat ctgttgctgg gcctaacgtg gtaacgattt ttgttctgcg aagccttttg
    13381 gacatgtaat actccgttga ctgaaacaac caggtgttgc ttgaacatga aatccgaatg
    13441 aaataacgcc gcgatggcga taaattaatc tcattcggat gtcgtataag attaggacag
    13501 tgacagtcgc ttttagcgat cgtcacttaa attaagtaac tgcttatcaa aacgcgattc
    13561 cttcagcgct tccttgaccc gcttcaagtt atctctgaat tttgcaccgc gtcgcaaagt
    13621 aaatcctgtc gccagcacat ctatcacggt cagctgtgca agtcgagaaa ccatgggcat
    13681 ataaatgtca gtatcttccg gtacgtcgag ggtaattgcc agcgttgctt cccgggcgag
    13741 cggggtaccc gcagaggtga gggcaatcac catggcgtcg ttttcgcgtg ccagctgcgc
    13801 cagctcgacc agattttttg ttcttcctgt gtgagaaatc agcaccacca cgtctccgtc
    13861 gctacaattc atacaactca tgcgttgcag cacgatatca tcggagtaca ccaccggaac
    13921 attaaaacga aagaacttat tcatcgcatc gtgggcaacg gcggcagaag agcctaatcc
    13981 gaaaaaggcg attttttttg cctgagtgag caagtcgacg gcgcggttga tggcagattt
    14041 atccagtgaa tgacggacat gatcgagcgt tgccattgcg gactcaaata ttttccccgt
    14101 gtatgattca acgctgtcat cttcattgac gttgcgatta acatagggag tgccattcgc
    14161 cagactttgt gccagatgaa gtttaaaatc aggaaaaccg cgcgtgtcca tgctgcgaca
    14221 gaaacgattc accgtcggtt cgctaacatt ggcttccagc gccagagcag caatactcga
    14281 atggatcgcg ttatcgggcg aagccagaat gacctcggca actttgcgct ctgatttgct
    14341 caaatgttcc agctgagact ggattttttc cagcatattc atgatgtaaa gagactcacg
    14401 ggtaatgacg atttccgcac tgaaagaaat cgaaatgcag ttttgtcaga tattacgcct
    14461 gtgtgccgtg ttaatgacaa aagcagataa aaaagttgtt atttttttca taacatgatc
    14521 agtgtcagat ttttaccgaa tggaaaacga tgattttttt atcagttttg ccgcgctttg
    14581 cgcgcttttc ccgtaatcgc acgggtggat aagcgtttac agttttcgca agctcgtaaa
    14641 agcagtacag tgcaccgtaa gaaaattaca agtataccct ggcttaagta ccgggttagt
    14701 taacttaagg agaatgacat ggcggtaacg caaacagccc aggcctgtga cctggtcatt
    14761 ttcggcgcga aaggcgacct tgcgcgtcgt aaattgctgc cttccctgta tcaactggaa
    14821 aaagtcggtc agctcaaccc ggacacccgg attatcggcg tagggcgtgc tgactgggat
    14881 aaagcggcat ataccaaagt tgtccgcgag gcgctcgaaa ctttcatgaa agaaaccatt
    14941 gatgaaggtt tatgggacac cctgagtgca cgtctggatt tttgtaatct cgatgtcaat
    15001 gacactgctg cattcaaccg tctcggcgcg atgctggatc aaaaaaatcg tatcactatt
    15061 aactactttg ccatgccgcc cagcactttt ggcgcaattt gcaaagggct tggcgaggca
    15121 aaactgaatg ctaaaccggc acgcgtagtc atggagaaac cgctggggac gtcgctggcg
    15181 acctcgcagg aaatcaatga tcaggttggc gaatacttcg aggagtgcca ggtttaccgt
    15241 atcgaccact atcttggtaa agaaacggtg ctgaacctgt tggcgctgcg ttttgctaac
    15301 tccctgtttg tgaataactg ggacaatcgc accattgatc atgttgagat taccgtggca
    15361 gaagaagtgg ggatcgaagg gcgctggggc tattttgata aagccggtca gatgcgtgac
    15421 atgattcaga accacctgct acaaattctc tgcatgattg cgatgtctcc gccatctgac
    15481 ctgagcgcag acagcatccg cgatgaaaaa gtgaaagtac tgaagtccct gcgccgcatc
    15541 gaccgctcca acgtacgcga aaaaaccgta cgcgggcaat atactgcggg cttcgcccag
    15601 ggcaaaaaag tgccgggata tctggaagaa gagggcgcga acaaaagcag caatacagaa
    15661 accttcgtgg cgatccgcgt cgacattgat aactggcgct gggccggtgt gccattctac
    15721 ctgcgtactg gtaaacgtct gccgaccaaa tgttctgaag tcgtggtcta tttcaaaaca
    15781 cctgaactga atctgtttaa agagtcgtgg caggatctgc cgcagaataa actgactatc
    15841 cgtctgcaac ctgatgaagg cgtggatatc caggtactga ataaagttcc tggccttgac
    15901 cacaaacata acctgcaaat caccaagctg gatctgagct attcagaaac ctttaatcag
    15961 acgcatctgg cggatgccta tgaacgtttg ctgctggaaa ccatgcgtgg tattcaggca
    16021 ctgtttgtac gtcgcgacga agtggaagaa gcctggaaat gggtagactc cattactgag
    16081 gcgtgggcga tggacaatga tgcgccgaaa ccgtatcagg ccggaacctg gggacccgtt
    16141 gcctcggtgg cgatgattac ccgtgatggt cgttcctgga atgagtttga gtaatatcag
    16201 cgcttatcct ttatggttat tttaccggta acatgatctt gcacagattg tagaacaatt
    16261 tttacacttt caggcctcgt gcggattcac ccacgaggct ttttttatta cactgactga
    16321 aacgtttttg ccctatgagc tccggttaca ggcgtttcac tcataaatcc tctgaatgaa
    16381 acgcgttgtg aatcatcctg ctctgacaac tcaatttcag gagcctttat gaatccacaa
    16441 ttgttacgcg taacaaatcg aatcattgaa cgttcgcgcg agactcgctc tgcttatctc
    16501 gcccggatag aacaagcgaa aacgtcgacc gttcatcgtt cgcagttggc atgcggtaac
    16561 ctggcacacg gtttcgctgc ctgccagcca gaaaacaaag cctctttgaa aagcatgttg
    16621 cgtaacaata tcgccatcat cacctcctat aacgacatgc tctccgcgca ccagccttat
    16681 gaacactatc cagaaatcat tcgtaaagcc ctgcatgaag cgaatgcggt tggtcaggtt
    16741 gcgggcggtg ttccggcgat gtgtgatggt gtcacccagg ggcaggatgg aatggaattg
    16801 tcgctgctaa gccgcgaagt gatagcgatg tctgcggcgg tggggctgtc ccataacatg
    16861 tttgatggtg ctctgttcct cggtgtgtgc gacaagattg tcccgggtct gacgatggca
    16921 gccctgtcgt ttggtcattt gcctgcggtg tttgtgccgt ctggaccgat ggcaagcggt
    16981 ttgccaaata aagaaaaagt gcgtattcgc cagctttatg ccgaaggtaa agtggaccgc
    17041 atggccttac tggagtcaga agccgcgtct taccatgcgc cgggaacatg tactttctac
    17101 ggtactgcca acaccaacca gatggtggtg gaatttatgg ggatgcagtt gccaggctct
    17161 tcttttgttc atccggattc tccgctgcgc gatgctttga ccgctgccgc tgcgcgtcag
    17221 gttacacgca tgaccggtaa tggtaatgaa tggatgccga tcggtaagat gatcgatgag
    17281 aaagtggtgg tgaacggtat cgttgcactg ctggcgaccg gtggttccac taaccacacc
    17341 atgcacctgg tggcgatggc gcgcgcggcc ggtattcaga ttaactggga tgacttctct
    17401 gacctttctg atgttgtacc gctgatggca cgtctctacc cgaacggccc ggccgatatt
    17461 aaccacttcc aggcggcagg tggcgtaccg gttctggtgc gtgaactgct caaagcaggc
    17521 ctgctgcatg aagatgtcaa tacggtggca ggctttggtc tgtctcgtta tacccttgaa
    17581 ccatggctga ataatggtga actggactgg cgggaagggg cggaaaaatc actcgacagc
    17641 aatgtgatcg cttccttcga acaacctttc tctcatcatg gtgggacaaa agtgttaagc
    17701 ggtaacctgg gccgtgcggt tatgaaaacc tctgccgtgc cggttgagaa ccaggtgatt
    17761 gaagcgccag cggttgtttt tgaaagccag catgacgtta tgccggcctt tgaagcgggt
    17821 ttgctggacc gcgattgtgt cgttgttgtc cgtcatcagg ggccaaaagc gaacggaatg
    17881 ccagaattac ataaactcat gccgccactt ggtgtattat tggaccggtg tttcaaaatt
    17941 gcgttagtta ccgatggacg actctccggc gcttcaggta aagtgccgtc agctatccac
    18001 gtaacaccag aagcctacga tggcgggctg ctggcaaaag tgcgcgacgg ggacatcatt
    18061 cgtgtgaatg gacagacagg cgaactgaca ctgctggtag acgaagcgga actggctgct
    18121 cgcgaaccgc acattcctga cctgagcgcg tcacgcgtgg gaacaggacg tgaattattc
    18181 agcgccttgc gtgaaaaact gtccggtgcc gaacagggcg caacctgtat cactttttaa
    18241 gacgacaaat ttgtaatcag gcgagagaaa actctgatga aaaactggaa aacaagtgca
    18301 gaatcaatcc tgaccaccgg cccggttgta ccggttatcg tggtaaaaaa actggaacac
    18361 gcggtgccga tggcaaaagc gttggttgct ggtggggtgc gcgttctgga agtgactctg
    18421 cgtaccgagt gtgcagttga cgctatccgt gctatcgcca aagaagtgcc tgaagcgatt
    18481 gtgggtgccg gtacggtgct gaatccacag cagctggcag aagtcactga agcgggtgca
    18541 cagttcgcaa ttagcccggg tctgaccgag ccgctgctga aagctgctac cgaagggact
    18601 attcctctga ttccggggat cagcactgtt tccgaactga tgctgggtat ggactacggt
    18661 ttgaaagagt tcaaattctt cccggctgaa gctaacggcg gcgtgaaagc cctgcaggcg
    18721 atcgcgggtc cgttctccca ggtccgtttc tgcccgacgg gtggtatttc tccggctaac
    18781 taccgtgact acctggcact gaaaagcgtg ctgtgcatcg gtggttcctg gctggttccg
    18841 gcagatgcgc tggaagcggg cgattacgac cgcattacta agctggcgcg tgaagctgta
    18901 gaaggcgcta agctgtaaaa gtcaaaatgc ccgatctagg atcgggcatt tttgtagcgt
    18961 ttttttgccg ggtttaaccc tgtactttta cctgtccggc ggcgtgcttc gcgcgttcaa
    19021 tggcgtcaac aacactctct gaagtagcca gtaccacccc cagatgacgg ctgccatcaa
    19081 tttccggctt accaaataaa cgaatctgca aatctgcgcc tacggcattc tgcacattat
    19141 caaacgtgac attctgactg gtcagttgtg gcagaataac ggcagaagct gcaggaccat
    19201 actgacggat cccgccaaca ggaagtccga ggaaggcacg tacatgcagg gcaaactctg
    19261 agagatcttg agaaattaac gtcaccatcc cggtatcatg tggacgaggg gagacctcac
    19321 tgaaaatcac ctcatcacca cagacaaaca gctcgacacc aaacagacca taaccgccca
    19381 gtgccagcac cactttacgg gcaatctcct gcgcacgttc aagggcaagc gggctcattt
    19441 gctgtggttg ccaggattca cggtagtcgc catcttcctg gcgatgacct actggtgcac
    19501 agaaatggac gccatccacc gcgctgacgg ttagcagggt aatttcgaag tcaaacttaa
    19561 cgacgccttc aacaattacg cggcccgctc cggcgcgacc gccttgctga gcgtattccc
    19621 atgcctgagc aagttgctct gcagagcgaa taaacgtctg ccccttgccg gaagagctca
    19681 tcaccggttt tacaatgcag ggatagccaa tggcggcaac cgcctcgcgg aaaaggcttt
    19741 tgctatcggc aaaacgataa gtggaagtgg gcagctgcag ctcttctgcc gccaggcgac
    19801 ggataccctc gcgattcatc gttaatttcg ttgcgcgagc gcaggggaca acattcagtc
    19861 cttcctcttc aagttggatc agcatatcgg tggcaatagc ttcgatctcc ggcacgatat
    19921 aatgtggttt ttccagttca accacacggc gtaatgcatc accatcaagc atattaatga
    19981 catgggagcg atgcgcgaca tgcatggctg gtgcgtcggc atagcgatcg acggcaatca
    20041 cctctacgcc gagacgctga cactcgattg ccacttcttt acccagttca ccggagccta
    20101 ataacatcac gcgagttgct gccggacgca gcgcagtgcc taataacgtc ataacgaata
    20161 tcccctgatt aattccgcgc gcagtataaa cgaaaacgtt tgcgtgtgtc tttattgctg
    20221 atgttgattt ctcaaccgaa aagaaatata ctgtataaaa tctcagttat tatgagaggc
    20281 tgcaaatggc ggttgaagtt aaatacgtag tcattcgtga gggcgaagag aaaatgtcgt
    20341 ttaccagcaa aaaggaagcc gatgcttatg acaaaatgct tgatacggcg gatctgcttg
    20401 acacctagct gacaaattct ccagtgcaaa tggaagacga gcaacgtgaa gccctttcgc
    20461 tatggctggc agaacaaaaa gaggtgctga gcaccattct gaaaaccggc aaattaccgt
    20521 ccccacaggt cgtcggtgct gaatccgaag aggaagatgc ctcacacgcg gcctgaagca
    20581 acttgcgctc ctgacaattt gtaccatgct ttctcctgaa tcattgtgct atggagaaaa
    20641 acatgaaaaa aagaggggcg tttttagggc ggttgttggt ttctgcctgc gcatcagttt
    20701 tcgctgccaa taatgaaacc agcaagtcgg tcactttccc aaagtgtgaa ggtctggatg
    20761 ctgccggaat tgccgcgagc gtaaaacgtg attatcaaca aaatcgcgtg gcgcgctggg
    20821 cagatgatca aaaaattgtc ggtcaggccg atcccgtggc ctgggtcagt ttgcaggaca
    20881 ttcagggtaa agatgataaa tggtcagtac cgctaaccgt gcgtggtaaa agtgccgata
    20941 ttcattacca ggtctgcgtg gactgcaaag cgggaatggc ggaatatcag cggcgttaat
    21001 ggcttctgat aattcgattg accgttggcg gatgcggcgc gagtgccttg tccgtcccag
    21061 aacgcaccac aatcgtaggc ctgagaagac gcaacagcgt cgcatcgggc aatcagcatc
    21121 aaacaatgcc actcaaaacg acaaactact ttcacctaaa atacccttat cacctgatat
    21181 acagtaaata ccttacctgg cgcgaatttc tttgaataac ggcctaccgc cccgcttatt
    21241 tgcgcatctt ttgtctcgat atacattttc tgacatcctc atccgctatg ctacaggcac
    21301 taaaaatggt tattgaggag caaatatggc taactggtta aatcaactgc aatccctgct
    21361 tgggcaaagc agttcttcta cctcctcgtc tgcggatcag ggattgggca aactgttagt
    21421 gtcaggcgca ttaggcggac tggctgggct gctggtcgca aataaatcag cacgtaaact
    21481 tcttacgaaa tatggcacca acgcgttact ggttggcggc ggagcggtag cgggtacggt
    21541 gctgtggaat aaatacaaag ataaaattcg cgcggcgcat caggacgaac cgcagtttgg
    21601 cgcgcaaagt acgccgctgg atgagcgtac ggaacgtttg atccttgcgc tggtctttgc
    21661 cgctaaaagt gatggtcata ttgatgccaa tgaacgtgcg gcaatcgacc agcaattgcg
    21721 tgaagccggc gtggaagagc aggggcgtgt actcattgag caggcaatcg aacaaccgct
    21781 ggatccacaa cgcctggcta ccggggtccg caatgaagaa gaggcgctgg aaatctattt
    21841 cctgagctgc gcggctattg atattgacca ttttatggaa cgcagttacc tgaatgcact
    21901 gggtgacgcg ctgaaaatcc cccaggatgt tcgtgaaggc attgaacgcg atctcgaaca
    21961 gcaaaaacgc actttggcgg aataattccg ctctctggat agcctgaagt tgactatttc
    22021 gggctatcca gaaccgcaca gccatttgaa caggctgatt ttctctgtct gcataacatt
    22081 cgatatccgc gccattacct gtcatcatct aagcaatgac gaccctgttt cgcttgcatc
    22141 cccggtgagt tttgccaccc ttataagatg tttccaacca gaaagaacaa taacatgcta
    22201 ccaaaagccg cccgcattcc ccacgccatg acgcttcatg gcgatacgcg catcgataat
    22261 tactactggc tgcgggacga tacgcgttct cagccaaaag tcctggacta cctgcaacaa
    22321 gaaaatagtt acggtcatcg ggtgatggcc tcacaacaag ccttgcagga tcgcatctta
    22381 aaggaaatca tcgaccgcat tccgcaaaga gaagtttctg cgccctacat aaaaaatggc
    22441 taccgctatc ggcatattta tgaaccaggc tgtgaatatg ctatctacca gcgtcaatcg
    22501 gcattcagcg aagagtggga tgagtgggaa acattgctcg atgccaacaa gcgcgcagct
    22561 catagtgagt tttattcgat gggcggaatg gcgattacgc ccgataacac cattatggcg
    22621 ctggcagaag attttctttc ccgacgccag tacggcattc gttttcgtaa tctggaaaca
    22681 ggtaactggt acccggaact gctggataac gttgaaccca gctttgtctg ggcaaatgac
    22741 tcctggactt tctactatgt tcgcaagcat ccggtgacgc tgctgcctta tcaggtctgg
    22801 cgtcacgcca tcggtacgcc agcatcgcaa gatacactga tctacgaaga aaaagacgat
    22861 acctattatg tcagcctgca taaaacgacg tcgaagcact atgtcgtcat tcatctggcc
    22921 agcgccacca ccagtgaagt tcgcctgctg gacgcggaaa tggccgatgc cgagccgttt
    22981 gtttttctgc cgcgccgcaa agatcacgaa tacagccttg atcactacca gcatcgtttt
    23041 tatctgcgtt ccaatcgcca cggcaaaaac tttggcttat accgtacccg tatgcgtgat
    23101 gagcaacagt gggaagagtt aattccgcca cgcgaaaaca tcatgctgga agggtttacg
    23161 ctgtttaccg actggctggt ggttgaagag cgtcagcgcg ggttaaccag tttgcgccaa
    23221 attaaccgca agacccggga agtcattggc attgcctttg atgatccggc ctatgtgacc
    23281 tggattgcct acaatccaga acctgaaacc gcgcgattgc gttatggtta ttcttccatg
    23341 accacaccag acactttgtt tgaactggat atggataccg gtgagcgtcg tgtattaaaa
    23401 caaacagaag tgcctggttt tgatgcggcg aattaccgca gtgaacacct gtggatagtc
    23461 gcccgtgatg gcgtcgaagt tccggtttcg ctggtctatc atcgcaaaca ttttcgcaaa
    23521 ggacacaacc cgttgctggt gtatggctat ggttcttacg gcgcaagtat tgatgccgat
    23581 ttcagtttta gccgcttgag tttgttagat cgtggctttg tctacgccat tgtccatgtt
    23641 cgcggcggtg gtgagctggg gcaacaatgg tacgaagacg gtaaatttct gaagaagaaa
    23701 aatacgttta atgattatct tgatgcctgc gatgcattgt taaaactggg ctatggctct
    23761 ccttcgctct gttatgcgat gggcggaagt gcggggggca tgttgatggg cgttgcaatt
    23821 aatcaacgcc cggaattatt ccacggcgtt atcgcccagg taccgtttgt tgatgttgta
    23881 acaacgatgc ttgatgaatc aattcctctt accactggtg agtttgaaga gtggggaaat
    23941 ccgcaggatc cgcaatatta cgagtacatg aaaagctaca gcccgtatga caacgtcacc
    24001 gcacaggctt atccgcattt actggtaacg accggtttac acgattctca ggtgcaatat
    24061 tgggaaccgg caaaatgggt cgctaaattg cgcgagctga aaaccgatga ccatctttta
    24121 ttgctctgta ccgacatgga ctcaggccat ggcggtaaat ctggccgctt taaatcgtac
    24181 gaaggcgtag cgatggaata tgcttttctg gtcgcgctgg cgcagggaac attacccgct
    24241 acgcctgcgg attaagtatt ttccagataa tgtttcagtg ttaaacgcag ctccgggctc
    24301 atgctgtcca ggttattaaa taaccagcgc agatagcccg gatagcgttc ggcaacgtcg
    24361 gaaaccgctt tgccacggta tttgccaaag gtgaaggttg tcatcaacga cggacgtccg
    24421 gtgatatcgg ccatctgttc tgccgtccag ccggaggtgt tcataatatc gataagcaac
    24481 gcggcggtga tataacagtc atacaacgcg cggtgatgat gcaggcccag cggtgtctgt
    24541 acattgagct tgcgtgtttt atataacgcc atattgctgt acttgagccc tggccacaaa
    24601 cgacgggcca gtttcatagt gcaaatccac tcaccgggca tctcaggcag tacgcggcgg
    24661 tcaaagctgg cgttgtgcgc gacataccat tcactaccgt aatagtgtgg gatcacatct
    24721 tcaatccacg gtttatcggc gaccatggct tcggtgatgc gatgaatcgc catcgcttgt
    24781 ggactaatag gacgatcggg gcgcaccagg aggctcatgg ggttgacgat ttttccgtca
    24841 atgacatcaa cagaggcaat ctcaacgatc cctccctgca aaccgcaggt ttctgtatcg
    24901 ataatgcgca acatggcatg atccaggccg aaaaagccta gcgtaatgga atgatatcgc
    24961 cttgccaacc ctgtgatgaa cgctgaccga ctaacaataa tgaaccacga tcggcgcgaa
    25021 cgatgagacg accatattca tcccatagtg cactgttacc acgggcattt gccatcagca
    25081 cggcaatcga gtactgatgt gaaaaaaatt gtaagcgacg ggccgacgcc agcaggtcgg
    25141 gttctcccag acattgccct gtcgtaaaca gtgaacaggt cgggtccata tccatacctt
    25201 gcggttgttc atcgaccacc gtgatggttc tggaacgacg gcccagacat gcaccatgac
    25261 tctgatggta gttcccaggc gtttttcgcc acggtgcgaa caccgcaatt ccacgaataa
    25321 agcgatcgtt atattcaacg ggaaggccgg caatgatggt cattcgctag gtcgttgcgg
    25381 cataacaaag cgggtctaac agtgaaagat cgggtggggc aggaagggca cgtcgcgaat
    25441 aatcacaccc cagtaaagaa agcgatggaa aaactaacag ctgacattgt tgcctggcgg
    25501 cggccctcac aaactccaga tgatgagcta cctgttcggt gagtgaggtt ttacgaggct
    25561 catattgcgc tgctgcaact ttccagaacg acataatgac ttccttttca aaatgactgc
    25621 aaattatggg ggcggtttag aaaatactca taatttagca ggcgttatgt aagaaagtgt
    25681 aactctgtat aagttttatt taagtttggg ctcgtaaggt aaacgtgaca gattgaccga
    25741 agccaaacgg tgggcaataa gccgctcgcg aaaccagctg cgcaaatgtt ctggctgttc
    25801 acgttcaacc gcttcagcga tcaccggcat attgtagcgt tctttaaaag ccaccccggc
    25861 cgccgccaaa tcgacattca ctttatccat ttctgtttga tccagtttag ccagattctt
    25921 cagcattctt aatctcctca tcattcgcgg gcaaaagtta ctaagagtga gcctggatgg
    25981 caagccggaa ttgatgatcc tcgactcaga cattagacaa ggttatgctc tgtaaaaaag
    26041 ttattacaaa aaggaataga taatatggct tcaactgcac gctcccttcg ctacgcgctg
    26101 gcaatcctga ccacttcgtt ggtgacacct tccgtctggg cacatgcgca tttaacgcat
    26161 cagtatcctg cggcaaacgc gcaagtgaca gctgccccgc aggcaatcac tttaaacttc
    26221 tcggaaggcg ttgaaacagg attcagtggt gcaaaaatca cggggccaaa aaacgaaaat
    26281 attaaaacat taccagcgaa gcggaatgag caggaccaaa agcaactgat tgttccactg
    26341 gccgattcgc tgaaacccgg aacctacacc gttgactggc atgttgtttc ggtggacggg
    26401 cataaaacga aaggacacta cacctttagc gtgaaataaa tcatgctggc gtttacctgg
    26461 atcgcgctgc gatttatcca tttcacctcg ttgatgctgg tctttggctt tgcaatgtat
    26521 ggtgcctggc tggccccctt aatgattcgt cgcttgctga cgaagcgttc tctgcgttta
    26581 caacagcacg ctgccgtctg gagtttgatc agtgccactg caatgctcgc cgttcagggc
    26641 gggttgatgg gaacgggatg gacagatgta ttttcgccaa acatttggca ggctgttttg
    26701 caaacgcagt ttggtggcgt ctggttatgg caaattgttc tcgccctcgt aacgttgatc
    26761 gtcgccctta tgcaaccgcg gaatatgcca cgtttgctgt ttatgctcac taccgcgcaa
    26821 tttattctgc tggcgggagt ggggtatgcg acgctgaatg aaggggtaac agcgaaaatc
    26881 catcagacta atcacgcgat tcacctgatt tgtgcggcgg cgtggtttgg gggattactg
    26941 ccggtgctct ggtgtatgca actcatcaaa ggccgttggc gacactaggc tattcaggcg
    27001 ctgatgcgtt tttcctggtg cgggcatttt gcggtgatcg gcgtactggc aagcggcgtg
    27061 cttaatgcat tgttgattac cggatttccc cccacgctta cgacttactg gggccaactc
    27121 ttattgctca aagccatcct ggtgatgatt atggtggtca ttgcgctggc taatcgttat
    27181 gttcttgtac cgcgcatgag gcaggatgaa gatcgtgcgg caccctggtt tgtgtggatg
    27241 actaaactcg aatgggcgat aggtgcggta gtgctggtga tcatcagcct gcttgcgact
    27301 ctcgaacctt tttgatggat tacgaaaatg atgaaaaaaa gtattctggc gtttctgtta
    27361 ctcaccagtt ctgcagcggc gctggcggct ccacaggtga taaccgtgag tcgttttgaa
    27421 gtgggtaaag acaaatgggc gtttaatcgc gaagaggtaa tgctgacttg ccgaccgggt
    27481 aatgctttgt atgtcatcaa cccaagtacc ctcgtgcagt atcctttaaa cgatatcgca
    27541 caaaaggaag ttgccagtgg gaagaccaat gcccaaccca tttcggtgat tcagattgat
    27601 gatcctaaca atcccggcga aaaaatgagt ctggcaccgt ttatagaacg agctgaaaaa
    27661 ctctgttaat tacctaaaat agccttttga tttccaataa aaaaaccgcc tcagttcttt
    27721 caccagaacg ggcggttttt aacatttaag ctgatgagca ccgcgctttt tattgaccat
    27781 tttgcacgca aactggaaaa cctggcgtcg tcatctattc ttaaagggca aggcaactaa
    27841 gcctgcatta atgccaactt ttagcgcacg gctctctccc aagagccatt tccctggacc
    27901 gaatacagga atcgtattcg gtcttttttt aattgtattt aaaatcaatt agttgcaaac
    27961 gtctccccga aattccccga aatttactcg aatttctgta ttccggtctt ttttggttat
    28021 atcacaacca aaatacattt aacaatccat ttacgttaaa atcagagcag taagtacgtt
    28081 ttttctctct catcaagata catttttgtt gtcttctccg atgtgtggcc aagtagacgc
    28141 tgagcaaatt cttctccaca tatttctttg tacaatcgtc cagccaggct tctgatttcg
    28201 tgaaaagttg gtgggttttc actgaacagt gatacctgtt aattttctgg ctgcgacaaa
    28261 tttctttgtc aggccgtccg ggtgaatgct gccgtcaggg ctgttttttc taatcccggc
    28321 actgattaga taatcccccc tacttaccat gcggcactgt tcaactactg caccaagtcg
    28381 taaaccagcg acaggaaggc tgagtgacag ggggatagca atcatcattc ctgtcttaat
    28441 ttgcctgatg tggagacgat catcataaat atcactgaac cgcatattcg ttatgtcttc
    28501 gcgacgttgt cctgttacaa gggctaagtc catagctaat gggaaccatg ccggaagttg
    28561 atctgctgcc tccctgatgc agttgtatgt ctttagtttc agccgttctc ttgtaactac
    28621 tattttcggt gctcttgttg gcgttactgg attttgagat atacgtcctt caacaatggc
    28681 ttcgcggaac atatcagata acacagaacg cattgatcct gccatcgtgt ttttccctcc
    28741 ttcaatctac aaatcaagaa actcggcgat atggcgagtg gttatttctg tcagtaaaac
    28801 ccctcctaat ttttctttta ttgtctccag ttgatttacc cgagttttat aagtatttct
    28861 ggacgctttt ctccttataa gaatcgtttt gtaacgttca atccagcctg ccatagtaaa
    28921 tgagtcgacc cctttaagct tttcaattag ggcagcagga gagtagtttt tgtatatata
    28981 atgatttgct tcaattgcct gcg
//
LOCUS       DAJTQQ010000046        28905 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000046 DAJTQQ010000000
VERSION     DAJTQQ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28905
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.046"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..2118)
                     /gene="yccS"
                     /locus_tag="OO309_002198"
     CDS             complement(<1..2118)
                     /gene="yccS"
                     /locus_tag="OO309_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR5847329.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQTLPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLKRATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQ"
     gene            complement(2128..2574)
                     /locus_tag="OO309_002199"
     CDS             complement(2128..2574)
                     /locus_tag="OO309_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR5847330.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2697..4751
                     /gene="helD"
                     /locus_tag="OO309_002200"
     CDS             2697..4751
                     /gene="helD"
                     /locus_tag="OO309_002200"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR5847331.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4783..5241)
                     /gene="mgsA"
                     /locus_tag="OO309_002201"
     CDS             complement(4783..5241)
                     /gene="mgsA"
                     /locus_tag="OO309_002201"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR5847332.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5337..5999)
                     /locus_tag="OO309_002202"
     CDS             complement(5337..5999)
                     /locus_tag="OO309_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR5847333.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6172..6585
                     /locus_tag="OO309_002203"
     CDS             6172..6585
                     /locus_tag="OO309_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR5847334.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6630..6947)
                     /gene="hspQ"
                     /locus_tag="OO309_002204"
     CDS             complement(6630..6947)
                     /gene="hspQ"
                     /locus_tag="OO309_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR5847335.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7005..8195)
                     /gene="rlmI"
                     /locus_tag="OO309_002205"
     CDS             complement(7005..8195)
                     /gene="rlmI"
                     /locus_tag="OO309_002205"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR5847336.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8290..8568
                     /gene="yccX"
                     /locus_tag="OO309_002206"
     CDS             8290..8568
                     /gene="yccX"
                     /locus_tag="OO309_002206"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR5847337.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8565..8894)
                     /gene="tusE"
                     /locus_tag="OO309_002207"
     CDS             complement(8565..8894)
                     /gene="tusE"
                     /locus_tag="OO309_002207"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR5847338.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8985..9644)
                     /gene="yccA"
                     /locus_tag="OO309_002208"
     CDS             complement(8985..9644)
                     /gene="yccA"
                     /locus_tag="OO309_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR5847339.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9851..9938)
                     /locus_tag="OO309_002209"
     tRNA            complement(9851..9938)
                     /locus_tag="OO309_002209"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9902..9904),aa:Ser,seq:tga)
     gene            10364..11482
                     /gene="hyaA"
                     /locus_tag="OO309_002210"
     CDS             10364..11482
                     /gene="hyaA"
                     /locus_tag="OO309_002210"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR5847340.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11479..13272
                     /gene="hyaB"
                     /locus_tag="OO309_002211"
     CDS             11479..13272
                     /gene="hyaB"
                     /locus_tag="OO309_002211"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR5847341.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13291..13998
                     /gene="hyaC"
                     /locus_tag="OO309_002212"
     CDS             13291..13998
                     /gene="hyaC"
                     /locus_tag="OO309_002212"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR5847342.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            13995..14582
                     /locus_tag="OO309_002213"
     CDS             13995..14582
                     /locus_tag="OO309_002213"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR5847343.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14579..14977
                     /gene="hyaE"
                     /locus_tag="OO309_002214"
     CDS             14579..14977
                     /gene="hyaE"
                     /locus_tag="OO309_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR5847344.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14974..15831
                     /locus_tag="OO309_002215"
     CDS             14974..15831
                     /locus_tag="OO309_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR5847345.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15965..17509
                     /gene="appC"
                     /locus_tag="OO309_002216"
     CDS             15965..17509
                     /gene="appC"
                     /locus_tag="OO309_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR5847346.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17521..18657
                     /gene="appB"
                     /locus_tag="OO309_002217"
     CDS             17521..18657
                     /gene="appB"
                     /locus_tag="OO309_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5847347.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18669..18761
                     /gene="cbdX"
                     /locus_tag="OO309_002218"
     CDS             18669..18761
                     /gene="cbdX"
                     /locus_tag="OO309_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR5847348.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18841..20139
                     /gene="appA"
                     /locus_tag="OO309_002219"
     CDS             18841..20139
                     /gene="appA"
                     /locus_tag="OO309_002219"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR5847349.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20254..22420)
                     /gene="etk"
                     /locus_tag="OO309_002220"
                     /pseudo
     CDS             complement(20254..22420)
                     /gene="etk"
                     /locus_tag="OO309_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22440..22886)
                     /gene="etp"
                     /locus_tag="OO309_002221"
                     /gene_synonym="yccY"
     CDS             complement(22440..22886)
                     /gene="etp"
                     /locus_tag="OO309_002221"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR5847350.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22874..24013)
                     /locus_tag="OO309_002222"
     CDS             complement(22874..24013)
                     /locus_tag="OO309_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR5847351.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24059..26155)
                     /locus_tag="OO309_002223"
     CDS             complement(24059..26155)
                     /locus_tag="OO309_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5847352.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26155..26901)
                     /locus_tag="OO309_002224"
     CDS             complement(26155..26901)
                     /locus_tag="OO309_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5847353.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26898..27542)
                     /locus_tag="OO309_002225"
     CDS             complement(26898..27542)
                     /locus_tag="OO309_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5847354.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27649..27954)
                     /locus_tag="OO309_002226"
     CDS             complement(27649..27954)
                     /locus_tag="OO309_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5847355.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28396..28608)
                     /gene="cspH"
                     /locus_tag="OO309_002227"
     CDS             complement(28396..28608)
                     /gene="cspH"
                     /locus_tag="OO309_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR5847356.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         7002 a         7317 c         7597 g         6989 t
ORIGIN      
        1 ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt gtacgaccag
       61 aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca ggccagctaa
      121 tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat caacatagca
      181 aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc ggtgagcacc
      241 gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa acgcggcttc
      301 gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg acgccagttc
      361 agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt tatcacgccc
      421 ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg tggcgcgttt
      481 gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca ctgccgccca
      541 cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt caaaaccttc
      601 acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg catgagcgta
      661 ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca ccagctgccc
      721 ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta ccagcgtacc
      781 aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc agacaaacaa
      841 acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga tggcgtagcc
      901 gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt ccggcgtgaa
      961 gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat cagcgagctc
     1021 attttcgtca ttattacggg gtagtgtctg ggcctgttct gattcaattg ttgccagttg
     1081 ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta aatcggcggg
     1141 tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag cgcgctcaaa
     1201 atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt gctggcacgc
     1261 ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt ggcgaaaatg
     1321 ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa tatcctgtgc
     1381 gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc cacgtaagcg
     1441 ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt tggcgagagc
     1501 caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca tgcgcgactt
     1561 gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct gcagcgggcg
     1621 gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg caccggccag
     1681 cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca tagtgtaaat
     1741 ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac cgccgagcaa
     1801 aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc agggaaacag
     1861 caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga ggttacgcaa
     1921 acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc ccagcgttag
     1981 cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc cacaaagcgc
     2041 aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt tgagcaaagg
     2101 acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat tggcttcacg
     2161 cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata gtgcaatagc
     2221 ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac attgtgcgat
     2281 gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc agaaaatatt
     2341 caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca gttcatcgac
     2401 atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc agcaggatcg
     2461 tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca gccagcccag
     2521 agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac gcataaaacc
     2581 tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg ggctggagca
     2641 cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac agagtcatgg
     2701 aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat cgggcggtga
     2761 tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt cctttcaatc
     2821 aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa tttgtactgc
     2881 cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag cgtttttacc
     2941 atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt gcgtctggtg
     3001 ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa tggctgacgc
     3061 gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg ttgccgttgc
     3121 cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa tgtcaggctt
     3181 ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc gaagccatgc
     3241 ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat ccggcgcagg
     3301 cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca ggaagcggaa
     3361 aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa gcgtcccctg
     3421 agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac gagcggattc
     3481 gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg gcgctgcata
     3541 ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat gataccgctg
     3601 cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag aaagcgcagg
     3661 cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca gaaggtaact
     3721 tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc tgggtaagtc
     3781 tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc gaagagattc
     3841 gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc tggaaaggtg
     3901 cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg attgtgattc
     3961 tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa tttcaggata
     4021 tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt cagacgacgt
     4081 tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg caaatgtcgc
     4141 tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta gacacgactt
     4201 accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag aacccaggcc
     4261 agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc acgttattgg
     4321 atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa ccggaagagc
     4381 gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa aaagcggcaa
     4441 cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa gggcaacagg
     4501 cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg gctgcggcgc
     4561 gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg gacgctgaag
     4621 aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg gcactgttta
     4681 acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg ccggtggcga
     4741 gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca gacggtcagc
     4801 gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat gcggcgactg
     4861 gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata ccgtcgccag
     4921 acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat cccagaagaa
     4981 tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt caccccccat
     5041 tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca agttaccggt
     5101 agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt ccacccagct
     5161 catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc gcgcaggtaa
     5221 agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca gattactgta
     5281 agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca aaaatctcag
     5341 gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg gtcagccagt
     5401 ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg aatagcatcc
     5461 agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc aaattgcggt
     5521 agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata atcaatcgtt
     5581 tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt cgcatcgcca
     5641 tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc attctccagg
     5701 cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac caccagcggt
     5761 ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc aacgcgaaac
     5821 actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag cagagagctg
     5881 gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg cagcgtggtg
     5941 gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc ggttttcata
     6001 ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc tttaatcata
     6061 cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat tagctgcatg
     6121 gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat aatgaaagaa
     6181 accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg ggcaagcgac
     6241 aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg gtatcacgtc
     6301 attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg gtatggcacg
     6361 ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc agaagcggcg
     6421 tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat gcaactgggg
     6481 gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt ggtgatggat
     6541 cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat ccccggaagg
     6601 caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc ctggagttgt
     6661 ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc atcctgcaat
     6721 tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt atcgtcctcc
     6781 atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag ctcatcgggc
     6841 gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag ataacctaac
     6901 agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt cacctcccgg
     6961 gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat gacgcgacag
     7021 gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg atcggctgcc
     7081 tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc atccgcgatg
     7141 attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag gagaataccg
     7201 ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg acacgcgccc
     7261 atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac gtcaaatttt
     7321 tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac aaactcagcc
     7381 ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag cgcttcctgg
     7441 gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac ggcgaaacca
     7501 ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg ggtagccagg
     7561 cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat atccaccagc
     7621 agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc ggtgacgagg
     7681 ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg atcgtaaatc
     7741 gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg atattctgcg
     7801 ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt aatacccggc
     7861 aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt ttgcgccagc
     7921 cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat gtcgatagac
     7981 tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga ataagcgccg
     8041 cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag gctggcttta
     8101 ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag taatgatttt
     8161 tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt cgatgcaaag
     8221 aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt aaggataagg
     8281 agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt tcagggcgta
     8341 ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg gtacgccaaa
     8401 aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca ggtagaaaaa
     8461 ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg ggtgctcagc
     8521 gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat acattttacc
     8581 ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg gaacagtcgg
     8641 tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc ttttaccagc
     8701 atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa acgcaccact
     8761 tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac tgccagcggt
     8821 tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat ctctttacct
     8881 tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta acaaaaaaca
     8941 aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc gcggctggcg
     9001 aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata caggctaacc
     9061 gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc aaacaagata
     9121 gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc aggcagttgc
     9181 aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat cagcataccg
     9241 ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca gaagaacact
     9301 aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga cagataggtg
     9361 ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc ggagataatc
     9421 ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat acccaccaga
     9481 gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc ggtaatcgcc
     9541 gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt atgggtgcta
     9601 agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc actctctatg
     9661 acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag aagttttacc
     9721 catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc attaagcaaa
     9781 tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt agctaacccc
     9841 ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg gtcgccggtt
     9901 ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc actataaaca
     9961 tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag ccaaaagtgc
    10021 attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc cttctctttt
    10081 actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat aggtccattt
    10141 ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt ttcactacgc
    10201 tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct ttcgttttca
    10261 gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc gaccataagc
    10321 gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata acgaggaaac
    10381 attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca aatattgtag
    10441 tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg cctgggcgct
    10501 ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta cctgctgtac
    10561 cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt ccctgatttc
    10621 cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag aagtctttga
    10681 agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta atccgccgct
    10741 gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga aactcaaacg
    10801 tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct ggggctgcgt
    10861 gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca tcaccgacaa
    10921 acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg ccatcattac
    10981 ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc cactgatgtt
    11041 ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg ccggagagtt
    11101 cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca aaatgggctg
    11161 caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg gcgtttcttt
    11221 cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct gggatcgcgg
    11281 ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca ccgccgatac
    11341 cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg cagttgccag
    11401 cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac atcagccagg
    11461 caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac catcaataat
    11521 gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat gcgctgtgaa
    11581 gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac catgtttcgc
    11641 gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt cgttgaacgt
    11701 atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat cgaagatgcc
    11761 atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct ggcaacgctc
    11821 tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg gatcgacgtg
    11881 ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag tctctcctct
    11941 tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa gaaattcgtt
    12001 gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca gtacaaactg
    12061 ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga tttccaacgt
    12121 gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa ctggattgtc
    12181 ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc ggtcaatatg
    12241 gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat taacaacgtg
    12301 atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga aatcggcacg
    12361 ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc caacgacttt
    12421 ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt caacaatgtg
    12481 ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca cgcctggtat
    12541 cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga cccgtggtac
    12601 aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga acaggaacgc
    12661 tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg gccgctggca
    12721 cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt cgatcgcatg
    12781 atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg cattttgtgc
    12841 cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga caagctgatg
    12901 accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga acctgcaacc
    12961 tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc gttaggccac
    13021 tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc gaccacctgg
    13081 aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc gctgatgaac
    13141 accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca cagctttgat
    13201 ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct gatctccgtg
    13261 caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa cgttgtcagc
    13321 cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt atgcatggcg
    13381 gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag tggcgaggcg
    13441 acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg gatgattttt
    13501 accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta ctcccgcgag
    13561 ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta tgaaatccgc
    13621 tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc catcgcccag
    13681 gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg ttttgcgctg
    13741 tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt ggaatttttc
    13801 tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg gatgtggctg
    13861 attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat catgtccgac
    13921 gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg caaaataagt
    13981 aacaaggagc gttcatgagc gagcaacacg tggtggtcat ggggctgggc aacctgctgt
    14041 gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat taccactggc
    14101 ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg gggtatgtcg
    14161 aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa cctggaacgc
    14221 tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa atgagcctgc
    14281 atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat ctgccagcac
    14341 atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc agcctgagcg
    14401 aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag ttggctgcgt
    14461 ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac tgtctgtcga
    14521 tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag gagcagggat
    14581 gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg gctggacgcc
    14641 ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg tggtgttatt
    14701 aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga ttggcgaatt
    14761 actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg agcagagcga
    14821 agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt ttaccggcgg
    14881 aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa acctgatgcg
    14941 cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt ccatctgctg
    15001 gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc gatcacctgt
    15061 caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag cccgcaggtg
    15121 attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc gttgggcgag
    15181 gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat caatacgctt
    15241 ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa aagtgaaata
    15301 caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga aaagttgctg
    15361 gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc aacgcaaaat
    15421 ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa tggcctaccg
    15481 ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca cagcattaat
    15541 ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct ctgtgggccg
    15601 ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc cacggggtta
    15661 cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact ggaaagttat
    15721 gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt cgactctgcg
    15781 cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta aaaagacggt
    15841 aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt cgtagagttt
    15901 caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc ctacaggagt
    15961 gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg cgctgtatca
    16021 ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca tggaaaccat
    16081 ttacgtggtt accggcaaaa caatctaccg cgatatgacg cgcttctggg gtaagctctt
    16141 cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc agtttggtac
    16201 taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac tggcgatgga
    16261 agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct tcggctggca
    16321 acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg gttcaaatct
    16381 ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg gtgcgcattt
    16441 tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct ttaacccggt
    16501 cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg ccatgtttat
    16561 tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg cattacgctc
    16621 gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc aactcggaga
    16681 cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga tggaagggga
    16741 gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg aacaggatca
    16801 agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg ccactcactc
    16861 attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc cacgcttgca
    16921 acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc gtgagccgca
    16981 tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt tgctctcccg
    17041 ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga tgcgtggcgc
    17101 gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct gtggttccct
    17161 gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa tcgaccagca
    17221 tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg cgattgaagc
    17281 cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca tcttaccgac
    17341 atactccgcg cactccgctt taaccacagg acaactggct ttctcactga tcatgatcgt
    17401 agggctttac accctgttct taatcgccga agtctacctg atgcagaaat atgcccgtct
    17461 ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa ggagaaaatc
    17521 atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt gatcctggtg
    17581 gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc gctggtggcg
    17641 cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg ggaaggcaat
    17701 caggtctggt tgatcctcgc aggtggggca ttatttgcag cctggcccag agtgtatgca
    17761 gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct gttcttccgt
    17821 ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa aatgtgggat
    17881 gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc cttcggcaac
    17941 ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta tctcggcagc
    18001 ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct tgggatggtg
    18061 attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca tctgcgttca
    18121 cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct ggcaggttac
    18181 tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa cggtccttcc
    18241 catccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa tttcgtcgaa
    18301 tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct gacggtgatg
    18361 gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat gcaatttggc
    18421 gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag tgtgagtcca
    18481 atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag cattatgttg
    18541 gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag ctactacaaa
    18601 atgtggggac gcatgacaac agaaactctc cgccgtaaca aaaacgagtt gtactaagga
    18661 gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt tcgctctcca
    18721 cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag gcctgatgcg
    18781 gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg aaacatatcg
    18841 atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc gcaatctgca
    18901 ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag tcgtcatggt
    18961 gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga cgcatggcca
    19021 acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat cgcctatctc
    19081 ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa gggctgcccg
    19141 cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa aacaggcgaa
    19201 gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca ggcagatacg
    19261 tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact ggataacgcg
    19321 aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt taccgggcat
    19381 cggcaaacgg tgtttcgcga actggaacgg gtgcttaatt tcccgcaatc aaacttgtgc
    19441 cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc atcggaactc
    19501 aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc aatgctgacg
    19561 gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg aaggatcacc
    19621 gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta tttactacaa
    19681 cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat catggcagcg
    19741 ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac ttcagtgctg
    19801 tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga gctcaactgg
    19861 acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt tgaacgctgg
    19921 cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca gactttacag
    19981 cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt gaaactgacc
    20041 ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg ttttacgcaa
    20101 atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa gagcattcag
    20161 ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt agaccggaaa
    20221 aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt aactataacc
    20281 gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac cattgaggat
    20341 tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc tcacctcttt
    20401 ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc gtcccacgac
    20461 cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat cgtaatggtc
    20521 gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag acgggttagg
    20581 tggcacctga ccgcgagtaa tcacatcaaa gtctcctttg ccaaaatgct ggatcacttt
    20641 gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac tcacggtaaa
    20701 caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt tttgatcgga
    20761 ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt ctggcgtcgc
    20821 accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca gactggttcg
    20881 tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca ggaaggggat
    20941 atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca gacgggtgcg
    21001 tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt gctcttccag
    21061 ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg cactcaccga
    21121 aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg gtttcactgg
    21181 ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac tggatttcga
    21241 aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc ccgcttctac
    21301 gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata cccgcttatt
    21361 caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac gataagttgg
    21421 gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct cattgagttg
    21481 attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa ggtcaaccga
    21541 atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct cgctgcgcac
    21601 ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg cctggcgagc
    21661 gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa tcaactgggg
    21721 atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt cgctaacggt
    21781 aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca ggacaaactg
    21841 tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt tttccagacg
    21901 ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt ggccgttttc
    21961 cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat gcatccagct
    22021 gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac ccacaatcgg
    22081 aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt taccgagaat
    22141 catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt tagggatcat
    22201 atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga ccagagtatc
    22261 tgcctgataa attggtgtgc ttaacagcga tcagcgtgaa taacgcggtc acgctgataa
    22321 taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc tcattttcct
    22381 gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat aaactcaaat
    22441 taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatact gtagacatgt
    22501 tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc cagccattgc
    22561 ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg agcaatatgt
    22621 tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt gagcttacgt
    22681 ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc tgtcgcatct
    22741 gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc cggcagacgt
    22801 ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca aaccactagg
    22861 attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct gtcaggtcat
    22921 gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga gccagcggcg
    22981 cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg agtatcattg
    23041 ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc tgcttgtttt
    23101 gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc ataccctcag
    23161 cgttacccag cgcttctgcc agggtcatac cgctgcggtc cattttcagc gtgctctgtt
    23221 tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata aataaaatat
    23281 cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc gcatagaggt
    23341 tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct gcatctgcgg
    23401 tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg gtaatcggct
    23461 gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg aacgccgcga
    23521 cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta atctcgttgc
    23581 gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg tagaaaatcg
    23641 caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc cctgcggggg
    23701 tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc ccgatacgat
    23761 attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata atcggtttgg
    23821 cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt ttgtccaggt
    23881 catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa ttctgaccag
    23941 gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa aacttaaaaa
    24001 tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt acgtcaggtt
    24061 attgtccaac cggtacttct cgctctgccg tcattggatc gagctggtat ttgcgtgaca
    24121 gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg ttgcggttag
    24181 gtccaatggt catcaagtcg aacggaatcg agatataaaa acctttacta aagccgcctt
    24241 cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc gccacgccgc
    24301 tgtcaaagag tttggcgacg tcgatcgttg cgcctttatc ttttgccaga tattgcccaa
    24361 cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc gtcacgaaac
    24421 ctgtgggcgt ggaataatcg gtgaagcgca tcatgttgtc ccagtcacgc tgcttcacgt
    24481 agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaaga agctcggaac
    24541 cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag aaaccattgc
    24601 ctaagtcggc aaagtaattc gcttgcaagt tgttgagata aacgtcattg cgaacgtaat
    24661 cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggtaacagt gaagacttaa
    24721 acttgtcgta gttgttgtaa atattggtga aaataccgtc atcaagcagc aggtggtcgg
    24781 taaaccagta gcgggcgctg gacatcagcc ccagctggaa catatagaaa tcttccgggc
    24841 cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct tcacgaatac
    24901 ggtagcaccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt tgcagttgct
    24961 ctgatttacc aggcgctgtg ccggccagct gtttgcgcag gctggctacg tcggtttcgg
    25021 tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca acgccttgcg
    25081 gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagag tcacggtatt
    25141 tgtattgctg accagacata tacagcgtct tatcgcgtag ctgaatttct ggcgcgtcaa
    25201 agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac tgcaaacctt
    25261 cagactctgg cgcaggctgc caggccggtt ttggcgtatc gcgtagggca gggcgcagat
    25321 cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt gtttccgcgt tcataactga
    25381 ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc gacgttgaaa tggcttgcct
    25441 gaggcagttt gccagcgaaa tcattctggt aattgttgcc gtcgtattcg agtttcagac
    25501 gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc gggccacgaa
    25561 agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga caatatttat
    25621 cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag ccccatgcca
    25681 tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatat tcaccgtcaa
    25741 acaaacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc cagtaacctt
    25801 cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga tcgccgctga
    25861 aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg gtgccttcca
    25921 gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta tcccggtagt
    25981 tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac aatcctgtgc
    26041 cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact tcagcatgac
    26101 aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc ttcatcagtc
    26161 aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat atttttcggg
    26221 cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt ccacatgtct
    26281 tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa tcaccataac
    26341 gttattcttg tctgcgcctg caaggcgggg gtgatcttgc agataatcgg taacgctacg
    26401 gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag taatagtcac
    26461 cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc tgttctcatc
    26521 cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat tgagattgag
    26581 taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt ctgcttccca
    26641 ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt tcgccgcaga
    26701 gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt gtgtcaccaa
    26761 ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgctag gcagataaat
    26821 agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc tggcaatgaa
    26881 atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac gggaataacc
    26941 cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc gacccagaat
    27001 gtgttttgcc actgtttttc tggcagcgtg gaagtcacct cttcctgcca gacattacac
    27061 agcacattgt gacccgcaat attcagcgtc tctaatccag caaaggaaaa agtagaactt
    27121 agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt ccagcggctg
    27181 ccatctttta ttgccagcgc gttacgcaga ggggcccgcc cgggatttgt cacttccagc
    27241 agattattat taagtttgac ggtctttaaa agacgtccat tgtgggtaac cagcatggcg
    27301 ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac cacaaagatt
    27361 cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg gatctgctga
    27421 gccgctacag tgatatcctg attatcgaaa aggctggcgc gaaatgtatc gaccatactt
    27481 tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat aagagggcgc
    27541 acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc ggtacgaata
    27601 tcgcaaccgg gtattattta acttgaattt cgacgcgttt aagcagaatt agcgcgtagt
    27661 ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg ctgcaactac
    27721 tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg tgccgacagc
    27781 gccagcagcc acgccactag tggtagccgc agtcgtggtt gcacctgtag tggtcgttgt
    27841 ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct gcttcagggg cagcaaacgc
    27901 agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt tcataacttt
    27961 tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct actttgagta
    28021 taacatcctt gtgaaatctc cctttcctca taccgctagc actatatact gcgggtggga
    28081 aaggcgcgca gagggaaata taagattgtt aacgatttcc ctctggaaat acgacttgtg
    28141 agcataagat aaatcttaga aaaatacgaa ttaaagataa gaaaattgtt acgaaagcta
    28201 ttaattattg ttggtaatat agtttcaagt ggtactataa ctggctgtta aatggcattt
    28261 ataactatta ggtgcagaga tattcgctta aatggattag tttcttagtg gaaaagtaac
    28321 tgatgttata atgtatggtg ttgactattg aactattcca ataaaaccag tcagctttaa
    28381 acaagcagcg tcatattaag agagataaac atttgccgct gttggtcctc gcaggccatt
    28441 tacgcggcaa aattccacgc gtaatcctgg tataagcact tctgcgtcgc ggggagtgaa
    28501 tgcggaaata tggacctgga cttctttacg accgtcggag gggataatga atcctttgcc
    28561 gcttttgcga tcaaaggttt tgacaattcc tgtcatttta cgggacaaac aaattcctta
    28621 ctgaaaatac tgcgctgcac tatacggggt taataaaata aagccagcga tatttaagac
    28681 cgccggacgg ctaaaataaa atttgcttaa tctcaattat catgccttaa tagctgcgtc
    28741 ggtttgaaag acagacagca tacaaagtag tttactaaag cagttctcat tatcaggcat
    28801 tatccccttc ttttgagtct ctctcctgaa cactaagtgg tttctgtatt aaagccctgt
    28861 ttgccgaaag gcccaaaatg aaggaagtaa aatatgtcta ataaa
//
LOCUS       DAJTQQ010000047        28531 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000047 DAJTQQ010000000
VERSION     DAJTQQ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.047"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..510
                     /locus_tag="OO309_002228"
     CDS             <1..510
                     /locus_tag="OO309_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5847357.1"
                     /translation="TIWEKAIFMRAIYLSVQQAWNGKITYSVSGESEFAKKFQGKALP
                     FDVRIISASQNEDWLVIATKVLPGADLRTYVDFKNSTVHVDSAGLEKVAKCINCNNTL
                     QVNIPHEAGHVLGYLDDDYDSSSPYVGDISGLMNVGMELWERYLKNATITLNIIMPET
                     KFTLLNVTK"
     gene            507..893
                     /locus_tag="OO309_002229"
     CDS             507..893
                     /locus_tag="OO309_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5847358.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            918..3131
                     /gene="tssI"
                     /locus_tag="OO309_002230"
     CDS             918..3131
                     /gene="tssI"
                     /locus_tag="OO309_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR5847359.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            3154..3599
                     /locus_tag="OO309_002231"
                     /pseudo
     CDS             3154..3599
                     /locus_tag="OO309_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3621..4028
                     /locus_tag="OO309_002232"
     CDS             3621..4028
                     /locus_tag="OO309_002232"
                     /inference="COORDINATES: protein motif:HMM:NF017315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HCR5847360.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            4025..6313
                     /locus_tag="OO309_002233"
     CDS             4025..6313
                     /locus_tag="OO309_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5847361.1"
                     /translation="MIEDGSPNVFLGGGTQTVLEISPEIPDWLRQVVDVLYVVAGMLG
                     GLAGAWRQAAKMGSKFGTKCAAKFIGGEMVGMAISDTVMGLFSNPVDVTTGQKILLPE
                     TDFTLPGRLPVTCSRFYASHLETEGLLGRGWRLNWEINLREDETYITFIGVQGRELSY
                     PKEMLIPGHQIFDPEEQFYLSRLHDGCYVLHYTDCSYYVFDEFDDHGVAPLLFMETPY
                     RQRIAFGRENGRLVRVASSSGHHLLLHRTMTQAGERLSHIELLKGGRPGNLVEYRYDD
                     NGQLTGVVNRAGVTVRQFAYENGLMTEHRNATGFTCTYHWEEIEGFPRVVEHTTSDGE
                     DYRFHYDFAGGQTVVTGRPEQKWQWWFDEETYVTAHRTPGGGMYRFTYNENHFPVAVE
                     LPGERRVTLEYDTLSRVVKETDPAGRVTQTQWNGSFAEITRRALDDDHVWKADYNEHG
                     QVIRETDPEGRVTRYGYDDQGLPETVCHPGKQQDRYTWNALGLLSSHRRITGSVQSWQ
                     YTQRGMLARHTDEEKRETRWQYTPEGLVASLSNGNGAQYRFSYDGDGRLTGEQRPDGL
                     IRMFALNADGFPVIIPTQGTEGGVRNEQQERDALGRLLRSDTQHSTRTFSYNRLDQIT
                     EVTLTPTEEGERLHHMQADTVRFAYDRSGWLTAEHSVHGSIKYRRDALGNPTDITLPD
                     GQHLSHLYYGSGHLLQTALDGITVSEYERDSLHRQVIRTQGKLATFSGYNADNRLSWQ
                     RSLPGGSQNPQQAVLPRRRTTA"
     gene            6310..7299
                     /locus_tag="OO309_002234"
     CDS             6310..7299
                     /locus_tag="OO309_002234"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5847362.1"
                     /translation="MTARDYIWNADGEVGGINDKLRGCLVFSYDRSGWLTSRTGQMYD
                     HDHYYYDKAGNLLTDEYQGAVMDNRLPGYGRDRYRYNEWGELTERRDQQLEWNAQGQL
                     TRVISSNSETRYQYDALGRRISKATSNLHTDRGERSRTTFVWEGFRLLQETTWQGKRT
                     YLYDAEQPYTPVAAITGRGESQKIWYYHTDLTGTVHEVTAPDGTLVWAGYQAGFGENR
                     GDISNSGAYFEQPQRLPGQYFDEETGLHYNLFRYYAPECGRFVSQDPIGLNGGLNLYA
                     YAPNPLGWIDPLGLNSLGIDSNNIFRGDGNYKGGGIGQPKSGTISAQDIIDHL"
     gene            7399..7602
                     /locus_tag="OO309_002235"
     CDS             7399..7602
                     /locus_tag="OO309_002235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5847363.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7620..7940
                     /locus_tag="OO309_002236"
     CDS             7620..7940
                     /locus_tag="OO309_002236"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5847364.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(8006..8238)
                     /locus_tag="OO309_002237"
                     /pseudo
     CDS             complement(8006..8238)
                     /locus_tag="OO309_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(8348..9118)
                     /gene="yafV"
                     /locus_tag="OO309_002238"
     CDS             complement(8348..9118)
                     /gene="yafV"
                     /locus_tag="OO309_002238"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR5847365.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            9299..9745
                     /gene="ivy"
                     /locus_tag="OO309_002239"
     CDS             9299..9745
                     /gene="ivy"
                     /locus_tag="OO309_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR5847366.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9788..12232)
                     /gene="fadE"
                     /locus_tag="OO309_002240"
     CDS             complement(9788..12232)
                     /gene="fadE"
                     /locus_tag="OO309_002240"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR5847367.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12472..13050
                     /gene="lpcA"
                     /locus_tag="OO309_002241"
     CDS             12472..13050
                     /gene="lpcA"
                     /locus_tag="OO309_002241"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR5847368.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13256..14023
                     /locus_tag="OO309_002242"
     CDS             13256..14023
                     /locus_tag="OO309_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR5847369.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(13994..14734)
                     /locus_tag="OO309_002243"
     CDS             complement(13994..14734)
                     /locus_tag="OO309_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR5847370.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            15118..15639
                     /locus_tag="OO309_002244"
                     /pseudo
     CDS             15118..15639
                     /locus_tag="OO309_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            15815..16312
                     /gene="rayT"
                     /locus_tag="OO309_002245"
     CDS             15815..16312
                     /gene="rayT"
                     /locus_tag="OO309_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR5847371.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(16529..18241)
                     /locus_tag="OO309_002246"
                     /pseudo
     CDS             complement(16529..18241)
                     /locus_tag="OO309_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            18213..18998
                     /gene="lafU"
                     /locus_tag="OO309_002247"
     CDS             18213..18998
                     /gene="lafU"
                     /locus_tag="OO309_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR5847372.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            19069..20124
                     /gene="dinB"
                     /locus_tag="OO309_002248"
     CDS             19069..20124
                     /gene="dinB"
                     /locus_tag="OO309_002248"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR5847373.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            20176..20469
                     /gene="yafN"
                     /locus_tag="OO309_002249"
     CDS             20176..20469
                     /gene="yafN"
                     /locus_tag="OO309_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCR5847374.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            20472..20870
                     /gene="yafO"
                     /locus_tag="OO309_002250"
     CDS             20472..20870
                     /gene="yafO"
                     /locus_tag="OO309_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCR5847375.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            20880..21332
                     /locus_tag="OO309_002251"
     CDS             20880..21332
                     /locus_tag="OO309_002251"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5847376.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            21578..22621
                     /locus_tag="OO309_002252"
     CDS             21578..22621
                     /locus_tag="OO309_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR5847377.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            22683..23297
                     /locus_tag="OO309_002253"
                     /pseudo
     CDS             22683..23297
                     /locus_tag="OO309_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(23354..24811)
                     /gene="pepD"
                     /locus_tag="OO309_002254"
     CDS             complement(23354..24811)
                     /gene="pepD"
                     /locus_tag="OO309_002254"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5847378.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            25073..25531
                     /gene="gpt"
                     /locus_tag="OO309_002255"
     CDS             25073..25531
                     /gene="gpt"
                     /locus_tag="OO309_002255"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5847379.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            25623..26867
                     /gene="frsA"
                     /locus_tag="OO309_002256"
     CDS             25623..26867
                     /gene="frsA"
                     /locus_tag="OO309_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5847380.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            26925..27326
                     /gene="crl"
                     /locus_tag="OO309_002257"
     CDS             26925..27326
                     /gene="crl"
                     /locus_tag="OO309_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5847381.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(27365..28420)
                     /gene="phoE"
                     /locus_tag="OO309_002258"
     CDS             complement(27365..28420)
                     /gene="phoE"
                     /locus_tag="OO309_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR5847382.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         7031 a         7109 c         7573 g         6818 t
ORIGIN      
        1 actatatggg aaaaagctat ttttatgcga gcgatatatt tatctgttca gcaggcatgg
       61 aatgggaaaa taacttattc tgtttccggt gaatctgagt tcgctaaaaa atttcagggt
      121 aaagctttgc cttttgatgt aagaataatt tctgcatctc agaatgaaga ctggttggtt
      181 attgcaacaa aggttttgcc tggtgctgat ttaagaacat atgttgattt taaaaacagt
      241 acggttcatg ttgattcagc gggcctggaa aaagtagcga aatgtattaa ctgtaataat
      301 acgttgcaag taaatatacc tcatgaagcc ggacatgtac ttggttactt agatgatgat
      361 tatgattctt cgtctccgta tgttggagat attagtgggt tgatgaatgt gggaatggag
      421 ctttgggaac gataccttaa aaacgcaact ataacgttga atatcataat gcctgaaact
      481 aaatttactt tattgaatgt aaccaaatga aatttttctg tattatagtg attttccttt
      541 caataaatta catagctata gtgaatgttt tcaagtcttc tgcagttgct caatccacca
      601 aatattatga agaaaatgat atacatgatg gaagtaatga catctttttt atgaagaaat
      661 atgggccgcc tgctcatgag ttcgtgaggc cacgatataa atttgttggt gagtattata
      721 tcggcttgaa tcaaacttat cgcataacag atcctgaatt tcaagatgtt cctattaagg
      781 aaatgttctg gaatttgcag ggggatttga atctgacatg ttggtttcat tataaagacg
      841 ggcaatggag agtgattact tatatttttt ggccaccagg ggctagattt taatccggta
      901 ttaaaggagc aaatatcatg agttttgtat ctacgggtaa taaaccagtt ggaaacggtg
      961 gacccgtcat caccacacca ccaatagctg gcgaaagtgg cggtatgagc acaggttcag
     1021 ctgtaaccga tgtatctggt gccgccgagg agatggcaga acaggctgcg gcggacctgt
     1081 ttggtgcgct gccggaaccg tcaggtctgg ttaaagccgc agtagctgct gcgcaggctg
     1141 ccgctgcagc agcagggatc tcggatatgg ctggtgcagt tcaggatgca gcggcgtcgc
     1201 ttgcagccgg tgctccgggg gcgcataacg tcacagtaag tggtagtgcg gtgccgccgc
     1261 agatgctttt gtttgcaggc atgaatggtt cggaagggct tgggaatctg ttttcttaca
     1321 cagtacagct taaaacgctg gacgccctga acctggggta cgtttccccc gctgctaacc
     1381 tgccgcttca gctcatggtg ggcaaagacc tgtgcgtcag tatcgaactg gacggcggcg
     1441 gaaaacggta tatcagcggg ctggtgacgg cggcgagggt ggtcggccat gagagccgtt
     1501 cggtaacgta tgaattacgc attgagccat ggctgaagct gctgacccat accagcgact
     1561 acaaagcgtt ccagaacaaa accgtggtgg atattctcga tgaagtactg gatgaatatt
     1621 cctttccggt ggaaaagcgg ctggtggaaa attatccacc ccgcgcctgg caggtgcagt
     1681 atggtgaaac cgacttcgat ttcatccagc gcctgatgca ggaatggggg atttactggt
     1741 ggtttgagca cagcgagaac agccacacgc tggtgctggt ggatgccatc aatggtcata
     1801 aagcgtgccc tgactcgccg ctggtggagt ggcaccagga agggctgaaa ctggataagg
     1861 aatttatcca caccattacg gccagtgaga ggctgcgtac tggaaagtgg gtgatggacg
     1921 attttgattt tatgaagcct cgttcgctgc ttaaaagtac cgtggccagt ccgcgtgata
     1981 ccggtcatgc tgaatacgag cattacgaat ggcctggtga ttacttcact acgggcgaag
     2041 gcgagatgct gacgcgcatt cgtatggaag cgcagcgcag cccaggcagc cgggcacatg
     2101 gggccggtca tatccgtacc ctcatgaccg ggtacacctt tacgttgatg aatcacccga
     2161 cggcagaaat taatcaggaa tatctgctgg tacagaccac tctgttttta cgggataatg
     2221 cgcagcacag cggtcaggat cagcatttta cctatgtcac cacgtttgaa ctgcacccca
     2281 ccagcgaagt ttaccgccca caacgaaccc tgagcaaacc ccacaccaaa ggcccgcaga
     2341 gcgccatcgt caccggcccg gcggggcagg aaatctggac ggataaatat ggccgggtga
     2401 aggtgcagtt tggctgggac cgttacggaa agaatgacga aaacagttcc tgctggatcc
     2461 gcgtcagcta tccgtgggcg ggtaaaggat tcggcatgat ccagatcccg cgtattggcc
     2521 aggaagtact ggtggatttc aaaaacggcg atccggattt gcccatcatc gtggggcgca
     2581 cctataacca ggacaccatg ccgccgtggg ggctgccggg catggcgtcg cagagtggga
     2641 tattcagcca ctcattgcag ggagggccga ctaacggaaa tatgctgcgc ttcgatgata
     2701 aaacgggtgc ggaagaggtg aagttccatg cggaaaaaga cctcaatacc acggtaaaaa
     2761 acaatgaaac ccacacggtc atggtcgatc gcataaaaac cattgtcaaa aatgaaacca
     2821 tcagcgtggg agaagaccgt aatgccacca tcacaaaaaa tgatggtctt tccgtcaaac
     2881 tggctcaaac catcaatgtc ggaacgacat atcgtctgga tgttggcgat caatttaccc
     2941 ttcgttgcgg caatgcagcg cttgtattac acaaggatgg ctcgattgag ttttgcggaa
     3001 aacaactgtt actgcacacc agcgatgtta tgcaactcat tggcagtggc attgatatga
     3061 atccggatgg cggcacagcc atgactgcgg atgatattgc cccgttacct acccccgacg
     3121 tttctgaata aaaaatataa aaggaatttt tctatgacca gcctttaccg tattcaggag
     3181 ggctgctttg ccctgcctga aacttttctt gatcgcaccg tcaatatctt cgtcccatcg
     3241 ggcaatgaaa gggcaactcc cagtctgaat attttccgcg atacgctcag gccagatgaa
     3301 aatctgacca cctatattga ccgccagata gccttgatga aaaaaaaaac ctaggccagc
     3361 accgggtact ttcgcgggga acggcactgg cgggaactgg cgaagctgcc atgacaggtg
     3421 aacagattct cgcaactcat cgttctggaa aaacagaagt gtaccagcgt caggccgggt
     3481 ttatcaccgg gccagcaaag gtactaatgt tgacgctgac cactcagcgt ccttttgatg
     3541 atcacaccga ccagctatgg actgcatggt tgactagttt tcagcctgct aaaagctaaa
     3601 aaaaacaggg aggttttgtc atgtatgaag ccgcacgggt ggatgacccc atctaccaca
     3661 ccagcgccct tgccgggttt ttgatagggg ccatcatagg tatcgccatt attgccgtcg
     3721 ccagctttgc tttttttacc tgcggctttt tggccgggct gattttgagt tttctggctg
     3781 atcagatagc ttccggagtc ctgcaactgg gtgaggctat tgggcgctcg atacattcca
     3841 cagtgggaaa aattctgacc ggttctgata atgtcagcac caacagtcgc ccggccgccc
     3901 gggccgtact gagtacggtg atatgcgaag accattcccc ggaaatccgc attgcccaag
     3961 gttccggcaa catctacatc aacagccagc tgcccgtaag gatgaccaca ctgagtgtga
     4021 tgcagtgatt gaggacggct cccccaatgt ctttctcggc ggcggtacgc agacggtact
     4081 ggaaatcagc ccggaaatcc ctgactggct gcgtcaggtg gtggatgtgc tgtatgtggt
     4141 cgccggaatg cttggcgggc tggctggcgc ctggcgtcag gcggcaaaga tggggtcgaa
     4201 atttggtaca aaatgtgcgg caaaatttat tggtggcgaa atggtcggga tggccatctc
     4261 tgacacagtc atgggattgt tcagcaatcc ggtagatgtc accactgggc aaaaaatttt
     4321 gctgccggag acggatttta ccctgccagg acgcctgcct gtcacctgtt cgcgctttta
     4381 cgccagccac ctggaaactg aaggattgct gggcagagga tggcggctga actgggagat
     4441 aaacctgcgt gaggatgaaa catatatcac cttcatcggt gtacagggtc gggaactgag
     4501 ttatccgaaa gagatgctga taccgggcca ccagatattc gacccggaag agcagtttta
     4561 cctgagccgt ctgcatgacg gatgctatgt gctgcattac accgactgca gctattacgt
     4621 gtttgatgaa tttgatgacc acggcgtcgc gccgttactt tttatggaga cgccgtaccg
     4681 gcagcgtatt gctttcgggc gtgagaacgg caggctggtg cgggtggcct ccagcagcgg
     4741 acaccatctg ttattgcacc gcacgatgac gcaagcaggg gaacgcctgt cgcatattga
     4801 acttttgaag ggcgggcgtc ccggtaacct ggtggaatat cgttacgacg ataacgggca
     4861 actgactggc gtggtgaacc gggctggtgt gacggtgcgc cagtttgcct atgaaaatgg
     4921 cctgatgaca gagcatcgca acgcgacggg ctttacctgt acctaccact gggaagagat
     4981 tgaaggtttc ccgcgcgtgg tggaacacac gaccagcgac ggcgaggatt accggttcca
     5041 ctatgatttt gctggcgggc agacggtggt aaccggcagg ccggagcaga agtggcagtg
     5101 gtggtttgat gaagagacgt atgtgactgc gcaccggacg ccgggcggcg gaatgtaccg
     5161 cttcacgtac aacgaaaacc attttccggt agcggtggaa ctccccggcg aacgcagggt
     5221 aacgctggaa tacgacaccc tgagccgggt ggtgaaagag acggacccgg caggccgggt
     5281 gacacagacg cagtggaacg gcagttttgc tgaaatcacg cgccgggcgc tggatgacga
     5341 ccacgtatgg aaggcggatt acaacgaaca cggccaggtg atacgggaga cggacccgga
     5401 agggcgcgtc acgcggtacg ggtatgatga tcaggggctg ccggagacgg tctgccaccc
     5461 cggtaaacag caggaccgtt acacctggaa cgcgctgggg ttactgagca gccaccgtcg
     5521 catcacgggc agtgtgcagt cctggcagta tacgcagcgc gggatgctgg cgcgccatac
     5581 ggatgaagag aaacgggaga cgcgctggca gtacaccccg gaagggctgg ttgccagtct
     5641 gagcaacggc aacggggctc agtaccggtt cagctacgac ggcgacggac ggctgacggg
     5701 agaacagcgc ccggacggcc tgataaggat gtttgcgctg aatgcggacg ggtttccggt
     5761 catcatcccg acgcagggca cagagggcgg ggtgcgcaat gaacagcagg agcgtgatgc
     5821 gctgggccgc ctgctgcgca gtgacacgca gcacagtaca cggaccttca gctacaaccg
     5881 tcttgaccag ataacggaag tgacgctcac ccccacggaa gaaggggaac ggctgcacca
     5941 catgcaggcg gacacggtgc gttttgcgta tgaccgcagc gggtggctga cggcagagca
     6001 cagcgtgcac ggcagtatca aataccggcg cgatgcgctg ggcaatccca ccgatatcac
     6061 gttgccggac gggcagcatc tcagtcatct ttactacggc agcgggcatc tgctccagac
     6121 ggcactggac ggtatcacgg tcagtgaata tgagcgtgac agcctgcacc gtcaggtgat
     6181 acgcacgcag gggaaactgg cgacgttcag cggctacaac gccgacaacc ggttaagctg
     6241 gcagcgcagc ctgccaggtg gcagccagaa tccacagcag gcggtactcc cccggcgcag
     6301 aacgacagcg tgacggcgcg ggactatatc tggaacgcag acggtgaagt cggcggcatc
     6361 aatgacaaac tgcgcggttg cctggtgttc agctatgacc gcagcggctg gctgaccagc
     6421 cgcacggggc agatgtatga ccatgaccat tattattacg acaaagcggg caacctgctg
     6481 acggatgagt atcagggagc ggtgatggat aaccgcctgc cgggttacgg gcgtgaccgc
     6541 taccgctata acgaatgggg cgaactgaca gagcgcaggg accagcagct ggagtggaac
     6601 gcgcagggcc agttaacgcg ggtcatcagc agcaacagcg agacgcggta tcagtacgat
     6661 gcgctgggca ggcgcatcag caaggcgacc agcaatctcc ataccgaccg gggagaacga
     6721 agccggacaa cgtttgtgtg ggaagggttc cggctgttac aggagacaac gtggcaggga
     6781 aagcggacat acctgtacga tgcggaacag ccgtacacgc cggtggctgc catcacgggg
     6841 cgcggagaga gccagaaaat ctggtattac cacacggacc tgacgggcac ggtacacgag
     6901 gtaaccgcgc cggacgggac gctggtgtgg gcgggctacc aggcgggatt tggtgaaaac
     6961 cggggcgaca tcagcaacag cggggcgtac tttgagcagc cgcagcggct gccggggcag
     7021 tactttgacg aagaaacagg gctgcattac aatctgttca ggtattatgc gccggagtgc
     7081 gggaggttcg taagccagga cccgatagga ttaaacggtg ggttgaactt atatgcttat
     7141 gcgcctaatc cgttaggttg gatcgatccg ctaggactga attctttagg aatagattca
     7201 aataatatat ttagaggaga tggtaactat aaaggtggcg gaattggaca gccaaagagt
     7261 ggcacaatat cagcacagga cattattgat catctctaag aaaaaggtga tgggcgttta
     7321 acatctttta gtactaaaaa aacatctagt tccaactctc gtggtgcaga ttttttctca
     7381 aaaaaacatg tcttcaaagt ggcagtttct gatctcgatg atttagttaa aaataatcag
     7441 atcactgttt taacacaaga aaaagtaaaa gcaatatttg gtttagaatc aagcaagaaa
     7501 attagaaaag aacttggtaa tattcttcag aatatggcta ggaataatga ggtgttagtt
     7561 catgggcaaa ttcttggaaa taaagttagc cgatgtaatt agttagtgga gaggaagttg
     7621 tggacgaaat tagaataaga attgctggta atacggctgc gccttgtttc catgtgctaa
     7681 aaaataaggg atataaagta acaatggaaa ctcatgcatt atctgacgat tgtttagatt
     7741 gtgtatatga ttggtatgcg gaaaaaaatg gacaattttt tagtgcaacg agtccagaag
     7801 agttacttgg attagttgca atgtgggaag ttcgtggtaa tgactggaga gctaatttga
     7861 atgagataaa tacttatcaa aaagccatag aagaatcccc gttttttgat aaaaacggca
     7921 atgaaataga gtctgactaa attttattga tatagcaacg ataataaccg gaaatttctt
     7981 ccagatcttt ccggcttttt attatttaac tttcgttcag cacactaaac atcccgtctc
     8041 gcatcccctt aactggctgt ttaacctgat aaagttccct gcgcagttcc tgtacttcgt
     8101 tgttatccca agcaggatca aacatccctc cgagatcttc acggtaacgc ccgtgccgat
     8161 ctcaaatccc gcttttctca gccatttggc gctgattttg atcaccggcg tgtcgctgcg
     8221 gttgggcaga taacccacgg tacagcgacg ctgtgttttg gaaatggtga cttctggcgt
     8281 aatattgtgt ttaagttagg ggctttcgta atctagccca gcccccggcc ctaaacacaa
     8341 cccccactca ccacaaccta aactcatccg catcccgcca tgccggaaac ttttctctat
     8401 attcccgcaa tgccaccatc gacagctccg catcaatacg cgttgcctta tgcgcgtcgg
     8461 cggtagcgat aatctcgcct tgcggattaa tcacccggct gtcaccgcga taatggcagc
     8521 cgttgccatc gctgccgacg cgattgcatc ccgccacata cgcctgattc tcaatcgcgc
     8581 gggccgtcag caatgcctgc cagtgcagag agcgcggagc tggccagttg gcgacgtaca
     8641 gggcgaggtc ataatcgttg agattgcgcg accacactgg aaaacgtaag tcgtagcaca
     8701 ccagcggcaa aatacgccag ccgcgccatt ccacaatcac tcgcgcattg cccgctttat
     8761 aatgcagatg ctcatctgcc atgcggaaca gatgacgctt atcataaaaa tgtaccgtgc
     8821 cgcccggctc aaccagcaaa aagcggttaa ccgaaccaga ctccgtttgt aatgcaacac
     8881 tgcctgcaat cagcgcattg cactgctgcg ccttagctgt catccagttc actacgtcat
     8941 cttgtgcaag cgacgaagct gccgcttcca tggcaaagcc gctggtaaac atctccggta
     9001 gaacgatcac atcgcgcccg gtaatacctt ccagttgacg atcaaaatgg cgcaggttgg
     9061 caggaccatc cacccacacc aacggttgct gcaaaagcgt aatcttcaaa ctaggcacgg
     9121 tgtacaactc ctttatgcga agggttttat aactttaaca ccttatcagg cagttgcctt
     9181 agcgcagaat aaattgataa caaatgctga tattggaaat atctgatttg caaattatcg
     9241 tgttatcgct aggctttagg aggttaataa catgggcagg ataagctcgg gaggaatgat
     9301 gtttaaggca ataacgacag tcgccgcact ggtcatcgcc accagtgcaa tggcgcagga
     9361 tgatttaacc attagcagcc ttgcaaaggg cgaaaccacc aaagctgcgt ttaatcagat
     9421 ggtgcaaggg cataagctac ctgcatgggt gatgaaaggc ggtacttata ctcccgcaca
     9481 aaccgtaacg ttgggagatg agacgtatca ggtgatgagc gcgtgcaaac cgcatgactg
     9541 tggctcgcaa cgtatcgctg tgatgtggtc cgagaaatct aatcagatga cggggctgtt
     9601 ctcggctatt gatgagaaaa cgtcgcaaga gaaactcacc tggctgaatg tgaacgatgc
     9661 gctttcgatt gatggtaaaa cggtgctgtt cgcggcgttg accggcagcc tggaaaacca
     9721 tccggatggc tttaatttta aataattagc ggataaagaa acggagcctt tcggctccgt
     9781 tattcaatta cgcggcttca actttccgca ctttctccgg caactttacc ggcttcgtcg
     9841 ccagctcttc cggatcaaag tcatcaacgt taatactgcg cagacggctt tcttccgctt
     9901 tcaccagaat agcggcttca tctttatcaa tcagcccctt cgtcagcgcg ttgtgcgcca
     9961 gttcatccag acgggtaaat ggcagatttt tacccagctc tttacagatc cgctgatgaa
    10021 tcgggtcggc ggcaatcaca tccaccaacg cctcttccag taaaccaacc gggttatgct
    10081 cgcttggcgt caggtactga ccgcgaccaa tacgtgaacg agtggcgttc ggcacttgta
    10141 aaatcttcgc cattttatga tccagcttgt cagacggtgc cagataatga cgtccggtcg
    10201 ggaagatcac cacattcagc agcccggcaa ccacgcggtt cgggaagttt tgcagtaaat
    10261 tgtccattgc ctgttctgcc tgatacagcg catcctgtac gccccagtgc accagcggca
    10321 gatcggcttc attacggcct tcgtcgtcat aacgcttcag aacggcagag gcgaggtaaa
    10381 gctggcttaa aatatccccc agacgtgccg agatacgctc acgacgtttc aggctgccgc
    10441 ccagcagtgc catcgagaca tcagaaagca gggcgaggtt ggcgctcagg cggttcaggt
    10501 gctgatagta gcgtttagtg gcatcgccgg ttggcgtgct gctggttaaa ccgcgcgtca
    10561 ggcccagcca gaagctgcga actttgttgc taccgacgtg accgatatgt ttgaacaaca
    10621 gtttatcgaa cgcgttgacg tcattgttct tcgccgcttc catctcttcc agcacgtacg
    10681 gatggcaacg aatcgctcct tgtccgaaga tcatcatgct gcgggtcaga atgttagccc
    10741 cttcaacggt gatggcaatc ggtgcgccct ggtaagcacg cgccaggaag ttgctttgcc
    10801 cgagcataat gcctttaccg ccggtaatat ccatcgcatc aataatcgac tgctgcccgc
    10861 ggtgggtaca gtgatactta acgatagccg acagcacggc aggtttttcg ccgagcataa
    10921 tgccgtaggt aatcagcgat gccgcagcat ccattacata agcgttcccg gcaatacgcg
    10981 ccagcggctc ttcaatccct tccatcttac cgatagagat tttgaactga cggcgaatgt
    11041 gagaatacgc gcccgttgcc agcgctaccg atttcacgcc gccggttgag ttggaaggca
    11101 gggtgatgcc gcggcctacc gagaggcact ccaccagcat ccgccagcct tgcccggcca
    11161 ttttcggccc gccgatgatg taatcgatcg gcacgaagac atctttaccg cgcgtcggtc
    11221 cgttctggaa cggtacgttc agcgggaagt ggcgacgacc aatttccacg cccggcgtgg
    11281 tggttgggat cagcgcacag gtaatgccta aatcttctgc accgccgagt aatttttccg
    11341 ggtcggagag tttaaacgcc agcccaagca cggtcgcaat cggtgccagc gtaatgtagc
    11401 gtttgttcca ggtcagacgc atccccagca cctgctggcc ctgccattcg cccatgcaga
    11461 caatcccggt gtccggaatc gcgcccgcat cggaacccgc ttccgggctg gtcagtgcaa
    11521 agcaggggat ctcctgacca cgcgccagac gcggcagata gtgatctttc tgctcgtcag
    11581 tgccgtaatg ttgcaacagt tcgcccgggc ctaatgagtt tggcacgccg acggtaatcg
    11641 ccaggatccc gctcacgcca gagagttttt gcagcacacg agactgggca taagctgaga
    11701 actccagtcc gccgtactct tttttgataa tcatcgcgaa gaagcgatgc tctttaaggt
    11761 acgcccacaa ctccggtggc agatccgcca gctcatgagt aatctggaaa tcattcgcca
    11821 tccggcaggc ttcttctacc gggccgtcga gaaacgcttg ctcttcagcg gtcaggcgcg
    11881 gctgcggata gttatgcagc tttttccagt ccggcttgcc ctggaacaag tcgccctccc
    11941 accaggtggt gcccgcatca atcgcttctt tctcagtgcg cgacatcggc ggcatcacct
    12001 tacggaaacc gcgaaatacc ggcgcggaaa tcatcgactt acgcataggc gcaaagttaa
    12061 atggcacgag gataatggtc agaggcacca gtacccacgc cgaccacaga ccagcaacgc
    12121 cgagggcggc tgtccaggcg agcaaaatca gactgctgat aaataagctc acgcggtgat
    12181 agaacaacgc gccgagcagg acaaccgtag cgagaatact caaaatcatc ataacgaaaa
    12241 gccccttact tgtaggaggt ctgaccactt gtgatgatat ggttgtagtg gatgtaaaaa
    12301 catttagcaa tgtgtttaca atataattac aacaaagctc acattgttgc tgtttttatc
    12361 cgcacttcag gtcaaaaagt cctggtcata gcacctgccc gtacttctcg cttttggcgg
    12421 tatccggtac actgcatttt gtctattaca tttatgctga aggatatcct catgtaccag
    12481 gatcttattc gtaacgaact gaacgaagcg gcggaaacgc tggctaactt tttaaaagat
    12541 gacgccaata ttcacgccat tcagcgcgcg gcggtcctgt tagcagacag ctttaaagcc
    12601 ggtggcaaag tgctttcctg cggcaacggc ggttcccatt gcgacgctat gcactttgcc
    12661 gaagagttga ccggtcgcta ccgtgaaaac cgtccgggct acccggcgat tgctatttct
    12721 gacgttagtc atatttcctg cgtcggtaat gatttcggtt tcaatgatat tttctcccgc
    12781 tacgttgaag cggtaggtcg cgaaggcgat gtactgctgg ggatctccac ctccggtaac
    12841 tctgcaaacg tgatcaaagc gatcgcagcg gcgcgtgaga aggggatgaa agtgatcacc
    12901 ctgaccggta aagacggcgg taaaatggct ggcacggcgg atatcgaaat tcgcgttccg
    12961 cactttggtt atgccgaccg cattcaggag attcacatta aagtgatcca tatcctgatc
    13021 cagttaattg aaaaagagat ggttaagtaa gtctggcgta ggccggataa ggcgtttacg
    13081 ccgcatccgg cacttgtgcg ctgatgcctg atgcgatgct gacgcgtctt atcatgccta
    13141 caaatctgta cccgaaccgt aggccgaata attcgtttac gccgcatccg acctgaaaat
    13201 tcttaaatca atcttcgccg gggtccatgc gctcccgctg ttgtggaggt tacccatgtg
    13261 cgaattgctc gggatgagcg ccaacgtccc taccgatatc tgctttagtt tcaccggact
    13321 tgtacagcgt ggtggtggaa ccgggccaca taaagatggc tggggcatta ccttttacga
    13381 aggtaaaggc tgtcgcacat ttaaagatcc acaacccagc tttaattccc ccatcgccaa
    13441 acttgtccag gactacccga taaaatcctg ttcggtggtg gctcatattc gccaggctaa
    13501 tcggggcgag gtggcgctgg aaaatactca cccatttacc cgcgagttat gggggcgtaa
    13561 ctggacttat gcccataacg gacaactgac gggctacaaa tcactggaaa ccggcaactt
    13621 ccgcccggtc ggtgaaaccg acagcgaaaa agccttttgc tggctgctgc ataaattaac
    13681 gcagcgttac ccgcgcacgc cgggcaacat ggcggcagtg tttaaatata tcgcctcact
    13741 ggcggatgaa ctgcgggcga agggcgtttt caacatgctg ctttcggacg ggcgctatgt
    13801 aatggcgtat tgctcgacta atttacactg gatcacccgc cgcgcgccgt ttggcgtggc
    13861 aacgttgctg gatcaggatg tggaaatcga cttcagctcg cagaccacac cgaatgatgt
    13921 ggtcacggtg attgcgacac agccgctgac gggcaatgaa acctggcaaa aaattatgcc
    13981 aggcgaatgg cgcttatttt gcctcgggga gcgtgtagtt tgatgccagt tgtggctgca
    14041 ccacttcgtg gcttaacggc ttgctgacca cgtaacggcc attggccaca gaaacggtcg
    14101 gtggcttacg ggtttgctca aagtagtcgt agcccggctt cagctgctcc cagaagtcct
    14161 taaagttgga atatttatgg cgcttcatat tggcgtcggt catgcggaat gggtaaatac
    14221 tcacctgcac gctcggctga ccaaacacca gcgcaccagt aacgaactgg aagatctcat
    14281 caataccctg attggtcatt gcgtagcagc cgatggatac acaatcgccg tggatcatca
    14341 ggtatttccc ttcataacca tgcgcacggt cataagcatt ggggaatcca atattaatcg
    14401 ctttgtagta acggctgtct ggttttaact gattacgctg gacgctataa aacccttccg
    14461 ggcttttgaa atcgccctga cgctgttttg gccctaagcc gccggaatat ttacagattt
    14521 tataactgtc gagcagttga tattgctcgc ccattttgac gtaaagatcg agcgtacgtt
    14581 cttccttgaa gatctggatg tagacagggg atcccatcaa ctgctgctta tactctttgc
    14641 tcactggcgt ggtcgagcta ctgctgccca gcaaaccggc aaacgaaacg cacgggatca
    14701 acaacatcgc aagaattaat gcgattttac gcatactgct tattccttga taaaacggtt
    14761 acacacgcca ggacggcaaa atggatccca aatcggaata gtctggattt ggaaggctca
    14821 cattatcacc aaaagagttt tacgcaagcc tgtcggcgct gggtttacaa atttagcaca
    14881 ggggcagttt tcaatagtcc gaaaagcgca tgtatcattt atttcctaat attatgaaat
    14941 ctcaatagta atatgcaaat aagcaattaa taatcccgtt tactcctccg gtcttttaaa
    15001 attctcacct tcattcagcc ggtttatgtc tttcatgtct tcgttcttgc tcggcagatt
    15061 tttgcatcca ggcgtgtttt cgttatgcgt tttgctccct ttactcgcca gcgccaccac
    15121 aaccgggagc agataaaaca gcatccagaa tggtttcctg cgccgctcaa ggcgagtgac
    15181 agacgctggc aggcgctggc ggaaaacaac cactttttaa gcagcgatca cctgcataac
    15241 atcaccgaag tggcgattca ccgcctggag cagcagcttg gcaagcctta cgtctggggc
    15301 ggcacgcggc ccgatcaagg cttcgattgt agtgggttag ttttttatgc ctacaacaag
    15361 atccttgagg cgaaactccc gcgcacggcc aatgagatgt accactaccg tcgggcaacg
    15421 attgtggcga ataacgacct gcgccgggga gatttgctat ttttccatat ccacagccgc
    15481 gagattgccg accatatggg ggtgtatctg ggcgacggtc agtttattga gtcactgcgc
    15541 accggggaaa ccattcgggt aagccggtta gccgaacctt tctggcagga ccatttttta
    15601 ggcgcgcgca ggattttgac ggaagagatg attttgtagg ccggataagg cgtttacgcc
    15661 gcatccggcg gttgtgcacg gatgcctgat gcgacgctga cgcgtcttat catgcctaca
    15721 aatctgtgcg tttttgcgag ccgctttccc gatataacac agcgtcattg tcggcctttc
    15781 cttttcctct taccgttgag aaaaaggagt cgctatgtcg gaatatcgtc gttattacat
    15841 caagggggga acatggtttt tcacggtaaa tttacgcaat cgtcgaagcc atcttctgac
    15901 cacccagttc cagacgctcc gtaacgccat tattaacgtt aagcgagaca gaccttttga
    15961 aattaacgcc tgggtcgttt tgccggagca tatgcattgc atatggacat tacccgaaag
    16021 cgatgatgat ttttcctctc gctggcggga gattaaaaag cagttcaccc atgcttgcgg
    16081 attgaaaaat atctggcaac cgcgtttttg ggagcatgcc attcgcaata caaaagatta
    16141 tcgccatcat gttgattata tttatataaa tccagagaaa catggctggg taaagcaggt
    16201 gagtgattgg ccattctcaa cgttccatcg cgatgtcgcg cgagggttat atcccatcga
    16261 ttgggcggga gacataacgg atttaagtgc cggggagcgt atcattttat aactgtgcgc
    16321 tggtgccgga tgcgcttacg cttatccggc ctacaaatcg tgctttaacg gtaggccgga
    16381 taaggcgttc acgccgcatc cggcagttat gcgcagatgc ctgatgcgac gctggcgcgt
    16441 cttatcatgc ctaaaaactt gtgcctgaac ggtaggccgg ataaggcgtt tacgccgcat
    16501 ccggcaattg tgcaccgatg ccggattctt acatcaacgc ccccataatc ttcaactcca
    16561 gctcatccgg cacttcgtta tacgacagca catgcagccc cggcgcaaac aaccttgcat
    16621 aacgcggcag caaagggcgt agctgcggcg gcaccagcag caccggatct ttccccgccg
    16681 ctttcatctg ctctttcacc tgtggcatcg tgctctggaa ctggttgagc atattcgggt
    16741 caaccggcac gctgtcgagc atcactttgc cggcctgttg cgcctgattc actacgttgg
    16801 tcagcagatt ttccagctca ttattcagcg tatacaccgt cagctcctgc ttgcgaacga
    16861 acggatgagt aatgctgcgc cgcagcgcca ggcgcacatc ggccgccagc agaatatgat
    16921 ctttagtcac cgtgctactg gcgaccagca cggtggcgat ggtgacgata tcgcgcaggg
    16981 aaacgccttc ggtcagcagc gcccggtaca ctttcagcaa ttggctgtaa ttgagcgccg
    17041 cgcttaaatc ctccgccagg cgcggtgcca tcgacgacaa acggttatgc aactgcgtga
    17101 tgtcatcata gttaaacaaa tcaggaatat agctgcgcac aatcttgttc acatgggtgg
    17161 caatcacact ggcgctgtcg atcacctgat accccatatt cagagccttc gctttctgcg
    17221 caggctggat ccaggtgacc ggtatcccgt acgccgggtc gttccccagc acgccgtcaa
    17281 tctcgccgta ggtttcgctg gagggcagcg ccatcagttt atccgccgga atatccgctt
    17341 catcagcctc aatgccgttg atgaaaatag cgtactggct gggcttgagg cggaagtttt
    17401 cccgaatgcg gatctccggc agcagcacgc cgttaccgtc ggaaatcacc tgccgaacgc
    17461 cgcgaatccg ctgggtgagc gggttgccct gcgctttgtc caccagcgcc accagcttgt
    17521 agccgaggct taagctgatg ggctcgatca gcggaatagt ttcccaactg acctgttgtt
    17581 cgctggtttc agtgatggtg cgggtcagcg tttcgaggct tttctcttcc gcctccgccg
    17641 cctgcgggcg tttgctcatc cgccagccgg taaagccaag cagggcgctg aacagcaaaa
    17701 acggcagatg cggcatcccc ggcaccaccg ccagcacgaa catgatcccg gtggcggtgt
    17761 agagcaccga cgggcttgcc agcaactggt ggcgcacgtc atgggcgata tcgccgttgt
    17821 cgctgacgcg ggtgacgata atcgccgccg cggtggagag cagcagggaa gggatctgcg
    17881 ccaccaggcc gtcgccgatg gtcatcagca cgtattgctg gaaggcggcg tcggcgctca
    17941 ggttgtattt gaagatgccg atacagacgc cgccgctcag gttgatcgcc agaatcatca
    18001 tcccggcgat ggcgtctccg cgtacaaact tcgatgcccc gtccatcgcg ccgtagaaat
    18061 cggcctcgct ggcaacatct ttacgccggg tttgcgcctg cgcctggttg atcaatccgg
    18121 cgttaagatc ggcgtcaatc gccatctgtt tgccgggcat cgcgtccagg gtaaagcggg
    18181 cagaaacctc ggaaatacgc tcggcctctt tggtgacgac gataaagttg attgtcaaca
    18241 gcgtcagtaa atccgagcgt gaaagcatta tcgccgcgct gcacgggcag tcgattttta
    18301 acggtggtgg gctgtcgccg ttaaataaaa tcagcccgtc tcatccgcca aaacctgcca
    18361 ccgtggcggt gccggaagag acggagaaaa aagcgcgtga tgtgaatgag aaaacggcgc
    18421 tgctgaagaa gaaatccgcc accgagcttg gcgagttggc aaccagtatc aacaccattg
    18481 cccgtgatgc gcatatggaa gcgaatctgg agatggagat tgttccccag ggattacgcg
    18541 tgctgattaa agacgaccag aatcgcaata tgttcgaacg cggcagcgcg cagattatgc
    18601 cgttctttaa aactttgctg gtggagctcg cgccagtgtt cgactcgctc tataacaaaa
    18661 ttattattac cgggcatacc gatgcgatgg cctacaaaaa caatatctac aacaactgga
    18721 acctttcggg cgatcgcgcg ctttcagctc gtcgggtgct ggaagaggcc ggaatgccgg
    18781 aagataaagt gatgcaggta agcgcaatgg cggaccagat gctgctggac gcgaagaatc
    18841 cgcaaagcgc gggcaatcgg cgtattgaga ttatggtgct gaccaaaagt gcgtccgata
    18901 cgctgtatca atactttggt cagcatgggg ataaagtggt gcagccgctg gtgcaaaagc
    18961 tggataagca gcaggtgctt tcgcagcgaa tgcgctaaat gctgaatctt tacgcatttc
    19021 tcaaaacctg aaatcactgt atactttacc agtgttgaga ggtgagcaat gcgtaaaatc
    19081 attcatgtgg atatggactg ctttttcgca gcggtggaga tgcgcgacaa tcccgctctg
    19141 cgcgatatcc ctattgctat tggcggcagc cgcgaacgtc ggggggtgat cagcaccgcc
    19201 aattatcccg cgcgtaaatt tggcgtacgt agcgctatgc ctactgggat ggcgctcaaa
    19261 ttatgcccac atctcacctt gcttccgggg cgctttgacg cctacaaaga agcctcaaat
    19321 catatccgtg aaatcttctc gcgctacacc tcgcgcattg aaccgttgtc actggatgag
    19381 gcttatctcg atgtcaccga tagcgtccat tgccacggtt ctgcgaccct catcgcccag
    19441 gaaatccgcc agacgatctt caacgagctg caactgacgg catctgcggg cgtggcacca
    19501 gtaaagtttc tcgccaaaat cgcctccgac atgaataaac ccaacggtca gtttgtgatt
    19561 acgccggcag aagttccggc atttttacaa accttaccac tggaaaaaat ccccggcgtc
    19621 ggcaaagtct cggcggcaaa actggaagcg atggggctgc ggacctgcgg tgatgtacaa
    19681 aagtgtgatc tggtgattct gcttaaacgc tttggcaaat ttggccgcat tttgtgggag
    19741 cgtagtcagg ggattgacga gcgcgacgtt aacagcgaac ggttacgaaa atccgtcggc
    19801 gtggaacgca cgatggcgga agatattcat cactggtctg aatgtgaagc gattatcgag
    19861 cggctgtatc cggaacttga acgccgtctg gcaaaggtaa aacctgattt actgattgct
    19921 cgccaggggg tgaaattaaa gttcgacgat tttcagcaga ccacccagga gcacgtctgg
    19981 ccgcggctga ataaagctga cttaatcgcc accgcgcgta aaacctggga tgaacgccgc
    20041 ggcgggcgcg gtgtgcgtct ggtggggctg catgtgacgt tgcttgaccc gcaaatggaa
    20101 agacaactgg tgctgggatt atgatgtata ctattatgta tattctggtg tgcattatta
    20161 tgagggtatc actgtatgca tcgaattctc gctgaaaaat cggtcaatat cactgagtta
    20221 cgtaaaaacc cagctaaata ctttattgat caaccagttg cggttctttc taataatcgc
    20281 cccgcaggat atctcttaag tgccagcgca ttcgaagcgt taatggacat gcttgctgaa
    20341 caagaggaga aaaagcccat aaaggcgcgc tttcgtccaa gtgctgcaag attagaggaa
    20401 attacacgcc gcgctaaaca atatcttaat gatatgacgg atgatgattt caatgacttt
    20461 aaggaataag gatgcgggta ttcaaaacaa aacttattcg cctgcaactt acagcagagg
    20521 aacttgatgc gttaacggcg gattttattt cctataagcg tgacggtgtt ttgccagata
    20581 tatttggtcg cgatgcactc tacgacgact cgtttacctg gccattaatc aaatttgagc
    20641 gagttgctca tattcatctg gcaaatgtga ataatccatt tccgccacag ttgcgccaat
    20701 tcagcagaac gaatgacgaa gcgcatttgg tatattgtca gggtgcgttt gatgagcaag
    20761 catggttgct cattgccatt ctgaagcctg aacctcataa actggctcga gataacaacc
    20821 aaatgcataa aattggaaaa atggcagaag cgtttcgcat gcgtttttga atttatatta
    20881 tgaataacat acaaataaga gattatcagc ctggcgattt tcagcaacta tgcgctattt
    20941 tcattagagc ggttacgatg accgtcagtc agcattattc actacaacaa attgccgcct
    21001 gggcgcagat tgacgaatct cgctggaagg agaaactcgc gaaatcacaa gtgcgggttg
    21061 cagttattaa tgcaaaactg gtcggcttta ttacctgcgt tgaacactat atcgatatgt
    21121 tatttgttga ccctgaatac acccgccgtg gggttgccag cgctttgtta aaacctttta
    21181 ttaagtctga atccgaactt acggtggacg caagcataac cgcaaaaccc ttttttgaac
    21241 gttatggttt tcagacagtt aagcagcagc gcgttgaatg ccggggagcg tggtttatta
    21301 atttttatat gcgttataaa ccgcaacatt aaatccagct tgcaatgaaa ataacgcccg
    21361 cctagtatgt gcgcgttttc ttattccaca aactgcaagg aggtaaatca tgacaaatcc
    21421 tttatcaatg actctttgca gacctttcca ggattaattc tttttttctt gccctggatt
    21481 cgtctgccat ttcctgattt tttaattgtt actctcaata attcaagtcg cagcacatgc
    21541 aacttgaagt atgacgagtc tatttacgga atggtatatg ggcaattata ttcgtccctt
    21601 atccgatgcg gtatttacca tcgcatctga tgacctgtgg atcgagagtt tagcgatcca
    21661 acaattacac accacggcaa atttacccaa catgcagcgc gtagtgggga tgccagattt
    21721 acaccccgga cgcggctacc cgattggcgc agcgttcttc tccgcaggcc gtttttaccc
    21781 agcactggtc ggcaatgata tcggctgtgg catggcgcta tggcaaacag atattctcgc
    21841 tcgcaaatac aacgccgata agtttgaaaa gcgattatca gctttggatg acgttgctga
    21901 agaaagctgg ctggaggaaa acctgccgtc agcatttgca cagcatccgt ggcgcagctc
    21961 gcttggctct atcggtggtg gcaatcattt cgctgaactg caacaagttg atcaaatcat
    22021 caacgctgaa ctgtttgcac tggcaggtct ggatgcgcag catctgcaac tgctggttca
    22081 tagcggctcg agggggctag ggcagtctat tttacagcgg catattgcct cgttttcgca
    22141 tcatggtttg cctgaaggca gtgacgacgc gctggcgttt gcgcgtttta atcgccacct
    22201 gatcgctttg cgcataatgc aacaggttaa ggccaccgga agtccggttc tggatgtggc
    22261 gcataacttt gttagcgcgt gtcgaatcgg tgatcaacaa ggggtcttac atcgtaaagg
    22321 tgccacgcct gatgactgtg gtctggtggt tattcccggt tcacgcggtg attactccag
    22381 gctggttcag cccgtcagga gtgaggaaac attgcattcg ctggcgcatg gggctgggcg
    22441 taaatggggg cgcaccgagt gtaaagggcg tctggcagcg aaatacacag cgacgcaact
    22501 ctcgcgtact gaacttggca gtcgggtaat ttgtcgcgat aaacaactca tcttcgaaga
    22561 agcgccacaa gcctataaat cggctgaaag tgtggtgcaa tgtcagggca ggccggatta
    22621 attattcctg tcgcgcggct gcgtccggtg ctaacgctca aaaacagtgg agggaaaaaa
    22681 ggatgatctt gctataactc tcctcagcgc aggggccgga agaatgttgt ctcgcagtga
    22741 aaaaggcgct ggacaggcta attaaagaag ctgcccgaca agacgtcgcg gtaacggtgc
    22801 tggaaacaga aacaggccgc tactctgaca cgctgcgttc ggcgctgatt tctctggatg
    22861 gcgacaacgc atgggcgtta agcgaaagct ggtgtggcac tattcagtgg atttgcccga
    22921 gcccgtatcg cccacatcac gggcgtaaaa actggtttct gggcattggg cgttttaccg
    22981 ctgatgagca ggagcaatcc gatgcgatcc ggtatgagac gctgcgttcg tcggggccgg
    23041 gcggtcaaca tgtcaataaa accgactcgg cggtacgcgc cacgcatttg gcatccggta
    23101 ttagcgtgaa ggttcagtca gagcgcagcc agcatgctaa caaacggctg gcgcgattgc
    23161 tgattgcctg gaagctggat cagcagcaat aggaaaatag cgcggtgctg aaatcacagc
    23221 ggcgaatgtt ccatcaccag attgaacgtg gcaacccgcg acgaacgttt acagggatgg
    23281 cttttatcga aggataatga aggatgaaac tgccggaagg cgattaaacg ccatccggca
    23341 gcaaatcaaa taattacttc gccggaattt ctttcagcag ttcagtcagc agtgtccagt
    23401 aatgacctac gctttcgatg tgaacttgct catccggaga gtgtggaccg gtgatagttg
    23461 gcccgataga aaccatgtcc atttccggat acggtttttt gaacagacca cattccaggc
    23521 ccgcgtggat aatctggatg ttcggcgtct tgttgaacag gcgctgatag gtttcacgta
    23581 ccagatgcat caccggagaa ttagcgtccg gctgccagcc aggatatgcg cctttcgctt
    23641 cggttttcgc gccagccagt ttacccagcg aatccagcat gctcaccacg tagtctttac
    23701 cgctgtcgat tagtgaacgg atcaggcagt gaatttctac gttattgtca gtcatggtca
    23761 ccacaccgac gttcagggag gtttcgacca caccttttgc cacgtcggaa ttgcggatca
    23821 caccgttcgg ggtggcgttc agcagacgaa taaaggtatc gcgagatttc gcaatcaggg
    23881 cagctttatc gttcgctaca gagtccagca acaaggccag atttttctct ttctctgcca
    23941 gctcgttttt caggatctcc tgataggtat tcaccagaga tttcagtgcg tcgactttat
    24001 cagctgcgac agcaatggtc gcaaaggctt cacgcgggat ggcgttacgc agtgtgccgc
    24061 cgttgaaatc gataaggcgc aggtccagtt cttctgcatg acccgccagg aagcgcacca
    24121 gcagtttgtt ggcattaccc aggccaacgt ggatttcacc gccggagtga ccgcctttca
    24181 gaccttttaa ggttaacttg aaggtttcaa aaccagctgg aaccgcttca cgatctaaat
    24241 gcaggttgga ggtgaagtcg ataccccccg cacaacccat gtagatttca ccttcttctt
    24301 cggagtcggt gttaatcaga atatcagcct gcaaccagtt gctctgtaag ccgaacgcac
    24361 cgtccatacc ggcttcttcg gtcatggtca gcagcacttc cagcgggccg tgaaccacgt
    24421 tttcgtcagc cagaaccgcc agtgcagagg ccataccaat gccgttatcc gcacccagcg
    24481 tggtgccgcg cgctttaacc cattcgccat caatataagg ctggatagga tctttcgtga
    24541 agtcatgcac ggtgtcgtta tttttctgcg gcaccatatc gaggtgggcc tgtaagacga
    24601 ccggtttacg attttccata cctgcggtag caggttgacg aatcaggata ttacctacct
    24661 gatcgcgttc gacatggaaa cctttctctt ttgcccaacc aacaatgtat tcagcgagtt
    24721 gctcttcatg ataggacggg tgaggaatag aacagatttt ggcaaaaata tcccacagcg
    24781 gctgtggaga taattgagac agttcagaca cgttaagtct ccttgtcgat cacccgcaaa
    24841 acagtattgc aggtcacagg gttagcagaa aatgttgtca acacaagaca ggcttgcgag
    24901 atatgtattg agaataccac tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc
    24961 ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg cgcaacctat
    25021 tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc acatgagcga
    25081 aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg caagccgact
    25141 gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc tggtaccggg
    25201 ggcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta
    25261 cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg
    25321 cttcatcgtt attgatgacc tggtggatac cggtggtact gctgttgcga ttcgtgaaat
    25381 gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga
    25441 tgactatgtt gttgatatcc cgcaagatac ctggatcgaa cagccgtggg atatgggcgt
    25501 cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcat tgccgggcgt
    25561 tgttcttttt aacttcaggc aggttacaat agtttccagt aagtattctg gaggctgcat
    25621 ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa
    25681 cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggctcttg
    25741 atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg
    25801 gtattgaccc acgtgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgctgaac
    25861 gtaccgacga tgatttatac gataccgtga ttggctaccg tggtggcaac tggatttatg
    25921 agtgggccac ccaggcgatg gtgtggcaac aaaaagcctg tacggaagaa gatccgcaac
    25981 tcagtggtcg acactggctg catgcggcta cgttgtacaa cattgccgcc tatcctcatc
    26041 tgaaaggaga tgacctggcc gagcaagcgc aggctttatc aaaccgcgcc tatgaagagg
    26101 ccgcccagcg tctgccgggc acgatgcggc agatggagtt taccgtaccc ggcggtgcgc
    26161 ccatcaccgg ctttttgcat atgccgaaag gcgatggccc gttcccgaca gtattaatgt
    26221 gtggtggtct ggatgcgatg cagacggact attacagcct gtatgaacgt tattttgcgc
    26281 cgcgcggcat tgcgatgctg actattgata tgccgtcggt gggcttttct tcaaaatgga
    26341 agctcaccca ggactccagc ctgttgcatc agcacgtctt aaaggcgctg cctaacgtac
    26401 cgtgggtgga tcacactcgc gtcgcggcct ttggtttccg tttcggcgct aacgttgccg
    26461 tgcgtctggc ataccttgaa tcgccgcgtc tgaaagcggt tgcctgtctt ggtccggtag
    26521 ttcataccct gttgagtgat tttaagtgcc agcaacaggt gccggaaatg tatcttgacg
    26581 ttctggcgag tcgtttgggg atgcatgatg cttccgacga agcgttacgc gtggagctga
    26641 atcgctattc attaaaagtg caaggattgc tggggcgtcg ctgcccaacg ccaatgttat
    26701 caggctactg gaagaacgat ccgttcagcc cggaagagga ctcacgctta atcacctcat
    26761 catctgctga cggtaaatta ttagagatcc catttaaccc ggtgtatcgg aattttgaca
    26821 aaggtcttca ggaaatcacc ggctggatcg aaaaacgctt gtgttaaaaa tttgctaaat
    26881 tttgccaatt tggtaaaaca gttgcatcac aacaggagat agcaatgacg ttaccgagtg
    26941 gacacccgaa gagcagattg atcaaaaaat ttaccgcact aggcccgtat attcgtgaag
    27001 gtaagtgcga agataatcga ttctttttcg attgtctggc tgtatgcgtc aacgtgaaac
    27061 cggcaccgga agtgcgtgaa ttctggggct ggtggatgga gctggaagcg caggaatccc
    27121 gttttaccta cagctaccag tttggtctgt tcgataaagc aggcgactgg aagagtgttc
    27181 cggtaaaaga tactgaagtg gttgaacgac tggagcacac cctgcgtgag tttcacgaga
    27241 agctgcgtga actgctgacg acgctgaatc tgaagctgga accggcgaat gattttcgtg
    27301 atgaaccggt gaagttaacg gcgtgagtga aatgtgccgg atgcatcaca tccggcaata
    27361 ttcattaaaa ctgatacgtc atcccaaccg cgacagtatc atcattatta atattcagct
    27421 tattatcgct atccagttgg ttgattttat aatcaacaaa cgctgacata tttttgttga
    27481 aataatacgt agcaccgacg tcgatataat tgaccagatc ttcatcaccg atgccctcaa
    27541 tatctttccc tttcgataag acataaccca gcgatggacg cagaccaaag tcaaactggt
    27601 attgagcgac cgcttcaaag ttctgtgtct tattggcaaa gccgccagtt attggcgtca
    27661 ttttgcgtgt ttcagaatag aaagttgcca gataaatatt attggcatcg tatttcagac
    27721 ctgttgccca tgcttctgca cgcttgcctg tgccacggct ttgcaggttc tgctcgttgg
    27781 tgcgatctga gttggtatag gccccactaa tggcgaaatc gctgccgcca aagtcatatg
    27841 tcaatgacgt gccgaagcca tcgccgtttt gctttttaac gtcgcggttt tcgtttttcc
    27901 cttgatattg cagggttaag ttcaggccat cgataacgcc gaagaagtcg gtgttccgat
    27961 acgtcgccag accgctggcg cgtttggtca taaagttgtc ggtctgcgcc gaggaatcgc
    28021 caccaaattc cgggaacata tcggtccagg cttccacgtc atacaacgcg cccaggttac
    28081 gaccataatc gaaagaaccc aaatctttat atttcaaccc ggcaaaagcg agacgcgttt
    28141 tttgctgtgc agtatcactc tccgctttat ttccggcaaa ctccgcttcc caccgaccat
    28201 aaccagtcag ttgatcgtta atttgagttt cacctttaaa accaaaacgg atataactct
    28261 ggtcgccatc tttactggcg ttatcactca tataatgcat ggctttaact ttgccataga
    28321 catccagttt attaccgtct ttattatata tttctgcagc ctgtacagat gcagatgcca
    28381 caatgcccat caccactaat gccagagtgc tctttttcat tttcattcct gattttaatt
    28441 aacgcgcgaa tattcagcgg gagagccccg ttgaaaacag gaaagttttt aacctgagat
    28501 tgttaaagat atattacaga ttaatgatat t
//
LOCUS       DAJTQQ010000048        28342 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000048 DAJTQQ010000000
VERSION     DAJTQQ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.048"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            556..1326
                     /locus_tag="OO309_002259"
     CDS             556..1326
                     /locus_tag="OO309_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR5847383.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1317..1790
                     /locus_tag="OO309_002260"
     CDS             1317..1790
                     /locus_tag="OO309_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR5847384.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1897..2436
                     /gene="dnaT"
                     /locus_tag="OO309_002261"
     CDS             1897..2436
                     /gene="dnaT"
                     /locus_tag="OO309_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR5847385.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2439..3176
                     /gene="dnaC"
                     /locus_tag="OO309_002262"
     CDS             2439..3176
                     /gene="dnaC"
                     /locus_tag="OO309_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR5847386.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3225..3719
                     /locus_tag="OO309_002263"
     CDS             3225..3719
                     /locus_tag="OO309_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR5847387.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3959..6250
                     /gene="opgB"
                     /locus_tag="OO309_002264"
     CDS             3959..6250
                     /gene="opgB"
                     /locus_tag="OO309_002264"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR5847388.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6388..7410)
                     /gene="lgoD"
                     /locus_tag="OO309_002265"
                     /pseudo
     CDS             complement(6388..7410)
                     /gene="lgoD"
                     /locus_tag="OO309_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7549..8463
                     /locus_tag="OO309_002266"
     CDS             7549..8463
                     /locus_tag="OO309_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5847389.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8678..10039
                     /locus_tag="OO309_002267"
     CDS             8678..10039
                     /locus_tag="OO309_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5847390.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10088..11752)
                     /gene="tsr"
                     /locus_tag="OO309_002268"
     CDS             complement(10088..11752)
                     /gene="tsr"
                     /locus_tag="OO309_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR5847391.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11871..12317)
                     /gene="hpaR"
                     /locus_tag="OO309_002269"
     CDS             complement(11871..12317)
                     /gene="hpaR"
                     /locus_tag="OO309_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR5847392.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12589..13878
                     /locus_tag="OO309_002270"
     CDS             12589..13878
                     /locus_tag="OO309_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR5847393.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13875..15341
                     /gene="hpaE"
                     /locus_tag="OO309_002271"
     CDS             13875..15341
                     /gene="hpaE"
                     /locus_tag="OO309_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR5847394.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15343..16194
                     /gene="hpaD"
                     /locus_tag="OO309_002272"
     CDS             15343..16194
                     /gene="hpaD"
                     /locus_tag="OO309_002272"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR5847395.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16204..16584
                     /locus_tag="OO309_002273"
     CDS             16204..16584
                     /locus_tag="OO309_002273"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR5847396.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16652..17455
                     /gene="hpaH"
                     /locus_tag="OO309_002274"
     CDS             16652..17455
                     /gene="hpaH"
                     /locus_tag="OO309_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR5847397.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17466..18269
                     /gene="hpaI"
                     /locus_tag="OO309_002275"
     CDS             17466..18269
                     /gene="hpaI"
                     /locus_tag="OO309_002275"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR5847398.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18291..19646
                     /gene="hpaX"
                     /locus_tag="OO309_002276"
     CDS             18291..19646
                     /gene="hpaX"
                     /locus_tag="OO309_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR5847399.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCPTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19656..20546
                     /gene="hpaA"
                     /locus_tag="OO309_002277"
     CDS             19656..20546
                     /gene="hpaA"
                     /locus_tag="OO309_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR5847400.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            20573..21510
                     /locus_tag="OO309_002278"
                     /pseudo
     CDS             20573..21510
                     /locus_tag="OO309_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21737..23295
                     /gene="hpaB"
                     /locus_tag="OO309_002279"
                     /pseudo
     CDS             21737..23295
                     /gene="hpaB"
                     /locus_tag="OO309_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23313..23825
                     /locus_tag="OO309_002280"
     CDS             23313..23825
                     /locus_tag="OO309_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR5847401.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24218..26368
                     /gene="btsT"
                     /locus_tag="OO309_002281"
     CDS             24218..26368
                     /gene="btsT"
                     /locus_tag="OO309_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR5847402.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26418..26621
                     /locus_tag="OO309_002282"
     CDS             26418..26621
                     /locus_tag="OO309_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5847403.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26632..27588
                     /gene="yjiA"
                     /locus_tag="OO309_002283"
     CDS             26632..27588
                     /gene="yjiA"
                     /locus_tag="OO309_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR5847404.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27657..>28342)
                     /locus_tag="OO309_002284"
     CDS             complement(27657..>28342)
                     /locus_tag="OO309_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HCR5847405.1"
                     /translation="GVDEWIPPVMAFMNRMARTEDFNLDDFSQFITAFEKVYMHGWLK
                     KQIKSQREMVCYSALVAINNDMPFDSVINQINQHADNSGFIAALDEDLYEPRPNQVNL
                     IKAILLRLDMEQQDESVIKTYTGRITIEHILPQALVNEYWINRFQPQEHVYWLHKIGN
                     LTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFYLTKDVCNSSEWGLAELKMRHEKMK
                     TQLKKLWLV"
BASE COUNT         6930 a         7416 c         7115 g         6881 t
ORIGIN      
        1 tttccagcaa attaatagat taagcgaata aattatcctt agcattcact ttcatatcag
       61 catactcctg ctgcacaaca actccactgc atgtgtcaga aggttagcat caaatgataa
      121 cctggtgaaa tagcctggag acatttaaaa tatctacatt aagttgttag aaatacttaa
      181 tgaaaaacag aaatctcatt catttaattt ataattaaat gatatctctt cccgcagaat
      241 gcgggaatca cacaatcaat atttattcaa taaaatctga tgattttaac ttgttaacac
      301 taaaaaacgc gcccagctat ctattatcct gacccgtaaa ggctaaacaa taaattcatt
      361 atagtgataa tattcatgtg atagtttgtt tgcggcgaga gataattcgc tttttatcac
      421 cgagcttgtc ccaataacca ggattaatca gcgcaatttt gatcttaacc taccgcaaaa
      481 caaccgtgct tcatgataca aatggattct cattatctga acaatacgca gcacgtctat
      541 gacaaagggc gagttatgca aactgagcaa cagcgagccg taacgcggct ttgtatccag
      601 tgtggattat ttcttttgca acatggtgcg gaaagcgcgt tggttgatga gctttcctca
      661 cgactgggtc gggcgctggg aatggacagc gtcgaaagtt ctatctcttc gaacgccata
      721 gtgctgacaa ctattaaaga tgggcaatgc ctgacatcga cacgtaaaaa tcacgatcgc
      781 ggcattaata tgcatgtggt gactgaagtc cagcacattg tgattcttgc ggagcatcat
      841 ctgctggatt acaaaggcgt agagaaacga tttagccaaa ttcaaccatt acgttaccca
      901 agatggctgg tagccttaat ggttggcctt tcttgcgcct gtttctgtaa actcaataac
      961 ggtggctggg atggtgccgt catcaccttc tttgccagta cgaccgcgat gtatatccgc
     1021 cagctgctgg cacaacgtca tcttcatcca cagatcaact tttgccttac cgctttcgcc
     1081 gccaccacca tttccggatt gcttttgcaa ctccccactt tcagcaatac ccccaccatt
     1141 gcgatggccg ccagcgttct gctgctagtg ccgggctttc cgttgattaa tgccgtcgcc
     1201 gatatgttta aaggccacat caataccgga ctggcacgct gggcgatcgc cagtctgctg
     1261 acactggcta cctgcgtcgg cgtagtgatg gcactgacga tttgggggct acgcggatgg
     1321 gtgtgatcga atttctgtta gcgttggcgc aggatatgat cctcgccgcc attcctgcgg
     1381 tcggctttgc gatggtgttc aacgttcccg tgcgggcgtt acgctggtgt gcgctgcttg
     1441 gctcgatagg tcatggttcc cgaatgatct tgatgaccag cgggttgaat attgagtggt
     1501 caacctttat ggcttctatg ctggtcggta ccattggtat tcaatggtcg cgctggtatc
     1561 tggcgcatcc gaaagtgttt accgtggcgg ccgttatccc tatgttcccg ggcatatcgg
     1621 cttataccgc aatgatttcg tcggtaaaaa tcagccagtt aggttacagc gaaccgttga
     1681 tgattaccct gttaaccaac tttcttacag cttcatcgat tgttggtgcg ttatccatcg
     1741 gtctttccat tcctggatta tggttgtacc gcaagcgccc tcgcgtataa aattgcctcg
     1801 cttaagcaca cggatgagag acagcctcct ctcctccgtg tgttactata aaagttatct
     1861 cccttctcgt tcatcgttcc atatttgaga aacagtatgt cttccagagt tttgaccccg
     1921 gacgtcgttg gtattgacgc cctggtacac gatcaccaaa ccgttctggc aaaagctgaa
     1981 ggcggtgtgg ttgccgtatt tgctaacaat gccccggcgt tttatgccgt cacgcctgca
     2041 cgcctggctg aactgctggc gctggaagaa aagctagcgc gtccgggaag cgatgtcgct
     2101 ctggacgatc aactctatca ggaaccgcaa gccgctcccg ttgctgtacc catggggaaa
     2161 ttcgccatgt atccggactg gcaacccgat gccgatttta tccgcctggc ggcgctatgg
     2221 ggcgtggcgc taagagagcc ggtgaccacc gaagaactgg cctcattcat tgcctactgg
     2281 caggcggaag gtaaagtctt tcaccatgtg cagtggcaac aaaaactggc gcgcagcctg
     2341 caaatcggtc gtgccagcaa cggcggactg ccgaaacgag atgtgaatac ggtcagtgaa
     2401 cctgacagcc aaattccacc aggattcaga gggtaacgat gaaaaacgtt ggcgacctga
     2461 tgcaacgcct gcaaaaaatg atgcctgccc atatcaaacc tgcattcaaa acgggtgaag
     2521 agctactggc ctggcagaaa gaacaagggg cgatccgctc cgccgctctc gaacgtgaaa
     2581 atcgggcgat gaaaatgcag cgcaccttta accgctccgg tattcgtcca ctgcatcaga
     2641 actgctcctt tgagaactat cgcgttgagt gtgaagggca gatgaatgcg ttaagcaaag
     2701 cgcgccagta tgtcgaagag tttgacggca acatcgccag cttcatcttt tctggtaagc
     2761 caggaaccgg caaaaaccat ctggcggcgg caatctgcaa cgagctgctg ctacgcggta
     2821 aatccgtatt gatcatcacc gtggccgata ttatgtcggc gatgaaagat accttcagga
     2881 atagcggtac cagcgaagaa caactgctta acgatctgag caacgttgat ctgctggtga
     2941 tcgatgagat cggcgtgcag accgaatcga aatacgaaaa agtgatcatc aaccagatcg
     3001 tcgatcgccg ctcttcttcc aaacgcccaa ccgggatgct gaccaacagt aatatggaag
     3061 agatgaccaa gttgctgggc gaacgcgtga tggaccgtat gcgcctgggt aacagtttat
     3121 gggtgatctt caactgggat agctaccgta gccgggtaac gggtaaagag tattaatccc
     3181 cgtttctggc ggtatactgc gacacaattc caccttgagt aatgatgatg aaaactatta
     3241 aacatcttct gtgctgtgcc attgccgcca gcgcattaat ttccaccggg gtgcatgctg
     3301 cgtcctggaa agatgcgctc tccagcgccg ccagcgaact tggcaaccaa aacagcacga
     3361 cacaggaagg cggttggtcg ctcgcgtcat taactaactt gcttagcagc ggaaaccaag
     3421 ccttaagcgc agataacatg aacaatgccg caggcattct gcaatactgc gcgaagcaaa
     3481 agctggcttc ggtaaccgat gccgaaaaca tcaagaacca ggtgctggaa aagctgggcc
     3541 tgaacagtga agagcaaaaa gaagacacca actatctgga cggtattcag ggtttgctga
     3601 aaacaaaaga tggtcagcaa ctcaatctgg ataacatcgg aacgactccg ctggcagaaa
     3661 aggtgaaaac caaagcctgc gatctggtgt taaagcaggg gctgaacttc atttcctgaa
     3721 ccgcgaaatg ttacccggct gacgcggctc tctcctgccc ttttccctgc tcctggacgg
     3781 ttttccccct gttacacggg aagcgaccgc gatcaaaaaa gtctgtttat aaactaattc
     3841 ctaacaacat cgcatttttt tgacactaga attgcagcaa tatggcgacg ccattacatt
     3901 gtgcttctca aaaagcttaa acgttggcta aacggcccgg tttagtgagg atcgcccatt
     3961 gtcagaacta ctctctttcg ccctttttct cgcctctgtg ctgatttacg catggaaagc
     4021 gggacgtaac acctggtggt ttgcagccac gttaacggtg ctggggctat ttgtcgtttt
     4081 aaatatcacc ctgtttgcca gcgactattt tactggcgat ggtattaacg acgcggttct
     4141 ctatacctta accaacagcc tgaccggtgc tggcgtcagc aaatacatcc tgccgggtat
     4201 cggcattgtg ctggggctga cggcggtgtt cggtgcgctg ggctggatcc tgcgccgtcg
     4261 tcgtcatcat ccgcaccatt ttggttacag cctgctggcg ctcttactgg cgctgggttc
     4321 ggtggacgcc agcccggcat ttcgtcagat aacggaactg gtgaaatccc agtcacgcga
     4381 tggcgacccg gactttgcgg cttattataa agagccgtcg aaaactatcc ctgacccgaa
     4441 actcaacctg gtttatatct acggcgaaag tctcgagcgg acctattttg ataacgaagc
     4501 tttcccggat ctcacgcctg aactgggcgc gttgaaaaat gaaggcctgg atttcagcca
     4561 cacgcagcag ctgccaggaa cggattacac gattgcgggc atggtggctt ctcagtgcgg
     4621 cataccgctg ttcgccccct ttgaaggcaa cgcctccgcc tctgtctcca gcttcttccc
     4681 gcagaacatc tgtctgggcg atatcctgaa aaactcgggt tatcagaact atttcgtgca
     4741 gggcgcaaat ctgcgttttg ccggtaaaga tgtgttcctg aaatcgcacg gcttcgacca
     4801 cttatacggc tcagaagagc tgaaaagcgt ggtagccgac ccgcactatc gcaacgactg
     4861 gggattctac gacgataccg ttctcgatga agcgtggaaa aagtttgaag agctttcccg
     4921 ctcaggtcag cgattctcac tgtttaccct gacagtcgat acccatcacc cggatggttt
     4981 tatctcgcgc acctgtaacc gcaaaaaata tgattttgac ggtaagccga atcagtcatt
     5041 cagcgcggta agttgcagcc aggagaatat cgcgacgttt atcaacaaaa tcaaagcgtc
     5101 accgtggttt aaagatactg tcatcgtcgt ctcttctgac catttagcga tgaacaacac
     5161 ggcgtggaaa tacctcaata agcaggatcg caataacctg ttttttgtca ttcgtggcga
     5221 caagccgcag caaaagacgc tggcagtgaa gcgtaacacg atggataacg gcgcgacggt
     5281 gctggacatt ctcggtggcg ataactatct cggacttggt cgtagcagtt tatccgggca
     5341 gtcgatgtcg gaaatcttcc tcaatatcaa agagaaaaca ttggcgtgga agccagatat
     5401 catccgcctg tggaaattcc ctaaagagat gaaagagttc accatcgacc agcagaaaaa
     5461 catgattgcc ttctcgggta gccatttccg tttgccgctg ctgttgcggg tttcagacaa
     5521 acgcgtggaa ccgctgccgg aaagcgaata ctcagcaccg ctgcgtttcc agctggccga
     5581 tttcgctcca cgcgacaatt tcgtctgggt tgacagttgc tacaagatgg cacaactctg
     5641 ggctccggaa ctggcacttt ccaccgactg gtgtgtctcg caggggcagc ttggcggtca
     5701 gcaaattgtt cagcatgttg acaaaacaac atggaagagc aagacggcat ttaaagatac
     5761 ggtcatcgac atggcgcgtt acaaaggcaa tgtcgatacg ctgaagattg ttgataacga
     5821 tattcgctac aaagccgaca gtttcatctt taacgtcgcc ggtgcgccag aagaggtgaa
     5881 acagtttagc gggatttccc gtccggagtc gtggggccgc tggtccaacg cgcagctggg
     5941 cgatgaagta aaaatcgagt acaagcatcc gctgccgaag aaatttgacc tggtgattac
     6001 cgccaaagca tacggcaata acgccagccg tcctattccg gtacgcgtag gcaatgaaga
     6061 acaaactctt gtgctgggca atgaagtgac caccaccaca ctgcatttcg ataacccaac
     6121 cgatgccgac acactggtaa ttgtgccgcc ggaacctgtc tcaaccaacg aagggaatat
     6181 cctcggacac tcgccgcgta agctcgggat cggcatggtg gaaattaaag tggtagaacg
     6241 tgaagggtaa ttcctgactg atgtgtaggt cgcatagggt gcgacctaca cgccttctcc
     6301 ggcttacaac acattattaa gaaacaaaaa aaacagcgcc caacacttcc ccggacgctg
     6361 catggcaact ttaataatct cgccttatca gaaagtaatt acacccttaa tcaactcacg
     6421 attgttaatc acatcgcgct cgtaggtttc tgccagcgtg gcgaacggat agcgatgggt
     6481 taacatcatg tcggcagtga ttttcccttc cgccatcagt cgaccgactt tagcgaaatc
     6541 ttccggcgtg gcgttgcggc tgcccatcat cgtcgtttct tttttatgga attccggatc
     6601 ggagaactgc aactcacctt taaacagacc gacaaatacc accgtgccgc cgtgacgaat
     6661 cagattcacg gtattattca tcgcatgttg attacctgtc gcgtcgatca ctttctgcgc
     6721 cagcgaacca ccaaactgcg cccgcagctg cgcgtcaaaa tcttcggctg acgggtccag
     6781 cacgggcaac tccagacgcg ttgccacatg ttcacggcgc gccggactgg tatccgccac
     6841 caccacctgt gcgccatcgg ctttagcgat tgccgccgcg cccagaccga ttggccccgc
     6901 cccgaccacc agcacctgct cgccgggagc aatggctgcg cgacgcaccg catgagcgct
     6961 aatagcgaaa ggttcaatca atgcggccgc ctgcgggtca ataccgtctg cgggcaaaat
     7021 gttcgccacc ggcacgctca aatactcact aaaaccgcca tcctgatgca cgccaatgac
     7081 tgaaattttt tcacagcagt tggtacgccc gcttttacac gccgggcatt gctgacaggc
     7141 aacataaggg atcacagcaa cttgctgacc atttttaaga tcagcaatat ttttacccag
     7201 cccaacaatc tccccacata tttcatggcc taaaacccgt ggataactaa aaaatggttg
     7261 atttccaccc taggcatgaa tatcggtacc gcaaatcccg acagacttaa tttttattaa
     7321 tgcttcattg tcacccggaa taggtatctc gcgttgtttc cagactaatt ctttcggctg
     7381 ctggcaaatt aaaacattca tcgtagacat gattcggcta tccatttttt gttgccttag
     7441 atatcaatga aaatgaaaaa tggcagcgga acggaaaatc tgttttgtga aaacacacgc
     7501 ataaaaatgt tttaaatcgg gttttaatgg actcacaagg aggaaaatat gagtcgttca
     7561 caaaatttac gccacaatgt gattaaccag gtcattgatg atatggcgcg gggtcatatc
     7621 ccttccccac ttccatcgca aagtgcgctg gcggaaatgt acaacatcag ccgcactaca
     7681 gtgcgtcata ttctcagcca attacgcgaa tgcggcgtct taacgcaggt tggaaacgac
     7741 tatgttattg cgcgtaaacc tgaccatgat gacggtttcg cctgtactac cgcttcaatg
     7801 agtgaacaaa acaaagtgtt tgagcaggct tttttcacga tgataaacca gcgtcagtta
     7861 cgccctaggg aaactttttc tgaactgcaa ctggctcggg ccgcgggtgt cagcccggtg
     7921 gttgtaagag aatatctttt aaaattcggc cgttataatc ttattcaaag cgaaaagcgc
     7981 ggccagtgga gcatgaagca attcgatcaa tcctacgccg agcagctgtt tgagctacgc
     8041 gaaatgctcg aaacacattc acttcagcac tttctcaacc tgcctgatca tgatccccgc
     8101 tggctgcagg cgaaaaccat gctagaacgt catcggctac tgcgtgacaa catcggcaat
     8161 agctttcgca tgttctcgca acttgacaga gattttcact ccttactgct ttcagctgcc
     8221 gacaatatct tttttgatca atcgcttgag atcatctcag tcatctttca ttttcactat
     8281 caatgggacg aaagcgatct taaacagcgc aatatcattg ccgtcgatga acatatgacc
     8341 attctcagcg ctctgatttg ccgcagtgac cttgatgcca cccttgcgct ccgtaaccat
     8401 ttgaattcag ctaaacaatc aatgattcga tccattaatg agaacacgcg ttatgctcat
     8461 taagtacgga ttaaaaacag caaactatta gcgggtgcac ggttttatca aaagataaat
     8521 accgggaaat tcccgcttac ctatgctcac aatcagtcga ctaaaaatgt ttttgtgagc
     8581 ggtagtaaag tcctaaaact ttaacctgcc gttaatgtca accttgcgta atgcacaatt
     8641 acgtaaacaa ttaaaattat aaatacagga gtccggcgtg gaaaaagaaa atatcaccct
     8701 cgatccgcgt tcttcattta ctccatcttc gtcggcagat attcccgtgc caccagatgg
     8761 attagttcaa cgcagtaccc gaattaaacg cattcaaacc accgccatgt tgttattatt
     8821 ttttgcggcg gtaatcaatt atctcgaccg cagttcgctg tcggtagcaa atttaacgat
     8881 tcgtgaagaa ttgggattaa gtgccaccga aatcggcgct ttgctctccg tgttttcact
     8941 cgcttacggc attgcgcaac ttccttgcgg cccactattg gatcgtaaag gcccacgcct
     9001 gatgctggga ctggggatgt tcttctggtc actgttccag gccatgtctg gcatggtgca
     9061 cagctttacg cagttcgtgt tggtgcgtat cggtatgggg attggtgaag cgccgatgaa
     9121 cccatgcggt gtaaaagtca ttaacgactg gttcaacatc aaagagcgcg gacgcccgat
     9181 gggcttcttc aacgcagctt ctaccattgg cgttgctgta agcccaccga ttctggcggc
     9241 aatgatgctg gtgatgggct ggcgcgggat gtttattacc attggtgtac tggggatttt
     9301 tctcgccatc ggctggtata tgctctatcg caaccgcgag cacgtagaac tgactgccgt
     9361 tgaacaagct tatctcaatg caggtagcgt caatgcccgc cgagatccgc tcaattttgc
     9421 cgaatggcgc agcctgttcc gtaaccgcac aatgtgggga atgatgctcg gattcagtgg
     9481 catcaactac actgcgtggc tgtatctggc ctggcttcct ggttacctgc aaacagccta
     9541 taacctggat ttaaaaagca cagggttgat ggcggctatc cctttcctgt ttggggctgc
     9601 cgggatgctg gtcaacggtt acgttactga ctggctggtc aaagggggaa tggctccgat
     9661 taaaagccgt aagatctgca ttattaccgg gatgttctgt tctgccgcct ttacgctgat
     9721 agtaccacaa gcgacaacat ccatgatggc ggttctgctg attggcatgg cactgttctg
     9781 tattcacttt gccggaacat cctgctgggg cttgatccac gtcgcagttg cttctcgcat
     9841 gactgcgtcg gtgggcagta tccagaactt tgccagcttc atctgcgcct ctttcgcgcc
     9901 gatcattact ggttttattg ttgatacaac ccactcattc cgtctggcac taatcatttg
     9961 cggttgcgtc accgcagcgg gagcactggc gtacatcttc ctggttcgcc agccgatcaa
    10021 cgacccgcgt aaagattaaa ctccctatgc ccgataacat tttgcttatc gggcattttt
    10081 atggcgatta aaacgtttcc cagttctccc cgctatctgc cactgccatt ttacgcggcg
    10141 tagctggcgt cacggttttt accacagccg atgtttcacg ctgctgttgc tgctgttgct
    10201 gaatccggaa cactgccaca gcttcggtca ggcgactggc ctgctcttcc agcgcggcgg
    10261 cggcagcggc agattcttcc accagcgcgg cgttctgttg agttacccgg tccatctcag
    10321 caaccgctaa gccaacctga tcgataccac ggctctgctc atcagaagca gaagctattt
    10381 cgcccataat gtccgtcacg cgggtcacag cactgacaat ctccgccatt gtttccccgg
    10441 cgctttcgac cagcgtagag ccaacatcca ctttccccac cgagtcttca atcaggcttt
    10501 taatttcacg aaccgcctgg gcgctacgct gggccagatt acgcacttct cccgcaacca
    10561 ccgcaaaacc acgtccttgc tcacccgcac gcgctgcttc aaccgccgcg ttcaaagcca
    10621 gaatattggt ctggaaggta atgccgtcaa ttacgctgat aatatcggcg attttctgcg
    10681 aactggtgga gatatcgcgc atagtctgca ccacgttatc taccactttg ccgccgcgtt
    10741 gcgccgtttc agaagcactt aatgccagat ggctggcctg gcgcgcattc tcggcgttct
    10801 gtttcaccgt tgcggtcagt tgctccatgc tggctgccgt ctcttccagc gaagcggcct
    10861 gttgctcggt gcgcgaagag agatcattat tgccggtagc gatttcgctg gcaccgctat
    10921 agatggcatt ggccccgttg cgcacatcac cgacggtacg catcagctct ccctgcatat
    10981 ggcgcaaact ctctgccagt tgccccatct cattagagcc atccacctca atcggtttca
    11041 ccagatcgcc gcctgcaata tgacgaatgc tgtcaatcag gcgattcatt ggcgctacca
    11101 gcgaggcttt aataccgaac cagacggcga agatgaccgc cagtacgacg atcatcacgc
    11161 ccaccagaat ccacatcgcc tggctgtagg aggcattgtt atcgctgacg gcgatatcat
    11221 agagccgatc gttttgctcc atgtaagcca catactgctt ctcgaaaccg tcctgatatc
    11281 cctgggtcgg ctgatcaaag aactcgttga ttttgcctgc acctaacagt tggatcagct
    11341 ccgccagcgc attgtgataa atatcgtaat tacgtttgat ctctgccgct gcggcggtgc
    11401 tctgacgcgg gtcacgcggc aacgcttcgt aatccgccca gtttttttcc gcctgtttca
    11461 gcgaaatact ggcactctgc atcagctcag caacggttga accgctacca atattattct
    11521 gatccatcat gtagcggata cccgcgcggt tgagggtgtt acgcgtctgc aacaacgcga
    11581 cccagctgcc attcagcgtg ggttgctgct ggcgaatggt ttgtaaaaca gtgaaatttt
    11641 ctttgtcatt ctttaaggca ttaaaaaaca gaccgcctga tgtcagttgt aaaaggccaa
    11701 aaacggccaa aaccagcagt aagctggtca cgatcttgat acgttttaac atggtttctc
    11761 tttcctgtgg accgatgcaa gattttcggc ctggaaagca aaaactttat gaaaaggtgt
    11821 aacccgccat ccatggaaaa accaggcgct ggttatcaga tactaaaaag ttattcatta
    11881 tcgcccggga tgtcttcttg tcgggaattg cccagagcaa taaattcctc taacagatgg
    11941 gttaactgtt gcattttctc ggcagtaaac tgagcttcaa tctgccgata ggcttcttca
    12001 atctgtgtct gagcgcggtt atacagcgcc tgcccctctt tggtcagcga gatatagagc
    12061 ttccgctgat cattgatcgg ctttaatcgc aacactaaac cgtcgcgttc catccgcgtg
    12121 aggatcccgg tcaggcttgg gcgcaaaatg caggcgcgat acgccagatc gtgaaaatcc
    12181 attgatgggc tttccgccag aatacgcacg atgcgccact gttgctcagt caaattatgc
    12241 cgtttaacga ttgggcgaaa ataactcatc gccgcttctc gcgcctggag caacgcaatg
    12301 gttagtgagt cgtgcattat ctttcccctg aaaaattcaa aatcattaat atagaaacaa
    12361 ttaatcatca cgttaccggg actgagctgc ccccgcaatt cttcatttta tcaaaattga
    12421 tgatatttta ttaacttact gatatttaat attttattat ctttaaatgt aaatagtttg
    12481 ttaattagat cacatttata tcacttactt ttgccaaact acgcaatcgg gaataaaagt
    12541 aatcattaac atattaatga ttaagatgat cgaatccgag gagtggtaat gaaaggcact
    12601 atcttcgccg tagcgttgaa ccatcgcagc caacttgatg cctggcagga agcgttccag
    12661 caatctccct acaaagcccc gcctaaaact gcggtctggt ttattaaacc gcgcaatacg
    12721 gtgattggtt gcggtgaacc gattcccttt ccacagggtg aaaaagtact gagcggtgcg
    12781 actgttgcgc tgattgtggg gaaaacggcg acgaaagtac gtgaagaaga tgcggcagag
    12841 tacatcgccg gatatgcgct ggataacgac gtcagcctgc cggaagagag cttttaccgt
    12901 ccggcaatca aagcaaaatg tcgtgatgga ttctgcccca ttggcgaaac cgtggctctc
    12961 agcaatgtcg ataatctgac tatctatacc gagatcaacg ggcgtcctgc cgatcactgg
    13021 aataccgccg atttacaacg taacgccgca cagctgctga gcgccctgag cgaatttgcc
    13081 acgctgaatc caggcgatgc tattctgctc ggcacgccac aggcgcgcgt ggaaatacag
    13141 ccaagcgatc gcgtgcgtgt tctcgcagaa ggtttcccgc cgctggaaaa tccggtagtg
    13201 gacgaacgtg aagtgaccac gcgcaagagc ttcccaacgc tgccacaccc gcacggtacg
    13261 ctgtttgccc tcggcctgaa ctacgccgac cacgccagcg aactggaatt taagccaccg
    13321 gaagagccgc tggtgttcct gaaagcgccg aataccctca ctggcgacaa ccagacctcc
    13381 gtgcgcccga acaatattga atacatgcac tacgaagcgg agctggtggt agttattggc
    13441 aagcaggcgc gtaacgtcag cgaagccgat gccatggatt atgtcgcggg ctacaccgtg
    13501 tgtaacgact acgccattcg cgactatctg gaaaactact accgccctaa cctgcgggtc
    13561 aaaagccgcg acggactgac gccgatgctt tcaaccatcg tgccgaaaga ggcgatcccg
    13621 gacccgcata atctgaccct tcgcaccttc gtcaacggcg agttacgcca gcaaggcacc
    13681 accgccgatc tgatcttcag cgtgcccttc ctgatcgcct acttaagcga atttatgacc
    13741 ctgaatccgg gcgacatgat cgccaccggc acaccaaaag gcttatctga cgtggtgcct
    13801 ggcgatgaag tagtggtgga agtagaaggc gtgggccgcc tggtgaaccg aattgtgagt
    13861 gaggaaacag cgaaatgaaa aaagtaaatc attggatcaa cggcaaaaat gttgcaggta
    13921 acgactactt ccagactacc aatccggcaa cgggtgaagt gctggcggat gtggcctctg
    13981 gcggtgaagc ggagatcaat caggcggtag cggcagcgaa agaggcgttc ccgaaatggg
    14041 ctgatctgcc gatgaaagag cgtgcgcgcc tgatgcgccg tctgggcgat ctgatcgacc
    14101 agaacgtgcc tgagatcgcc gcgatggaaa ccgcggacac gggcctgccg atccatcaga
    14161 ccaaaaatgt gttgatccca cgcgcttctc acaactttga atttttcgcg gaagtctgcc
    14221 agcagatgaa cggcaagacc tatccggttg acgacaagat gctcaactac acgctggtgc
    14281 aaccggtagg cgtttgtgcg ctggcgtcgc cgtggaacgt gccgtttatg accgccacct
    14341 ggaaggtcgc gccgtgtctg gcgctgggca ataccgcggt gctgaagatg tcggaactct
    14401 ccccgctgac cgccgaccgc ctgggtgagc tggcgctgga agctggtatt ccggcgggcg
    14461 tgctaaacgt ggtacagggc tacggcgcaa ccgcaggcga tgcgctggtc cgtcatcatg
    14521 acgtgcgtgc cgtgtcgttc accggcggta ccgccaccgg gcgcaacatc atgaaaaacg
    14581 ccgggctgaa aaaatactcc atggagctgg gcggtaaatc accggtgctg atttttgaag
    14641 atgccgatat tgagcgtgcg ctggacgccg ccctgttcac catcttctca atcaacggcg
    14701 aacgctgcac cgccggttcg cgcatcttta ttcagcagag catctatcca gaattcgtta
    14761 aacgctttgc cgaacgcgcc aaccgtctgc gcgtgggcga tccgaacgat ccgaataccc
    14821 aggttagggc gcttatcagt cagcaacact gggaaaaagt ctccggctat atccgtctcg
    14881 gcattgaaga aggcgcaacc ctgttggcgg gcggcccgga taaaccgtcc gacctgcctg
    14941 cgcacctgaa aggcggcaac ttcctgcgcc caacggtgct ggcggacgta gataaccgta
    15001 tgcgcgttgc ccaggaagag attttcgggc cggtcgcctg cctgctgccg tttaaagacg
    15061 aagccgaagg cttacgcctg gcaaacgacg tggagtacgg cctcgcgtcg tacatctgga
    15121 cacaggatgt cagcaaagtg ttacgcctgg cgcgtggcat tgaagcaggc atggtgttcg
    15181 tcaacaccca gaacgtgcgc gacctgcgcc agccatttgg cggcgtaaaa gcctccggca
    15241 ccgggcgtga aggcggtgag tacagcttcg aagtgttcgc ggaaatgaag aacgtctgca
    15301 tttccatggg cgaccatcca attccgaaat ggggagtctg atatgggtaa gttagcgtta
    15361 gccgccaaga tcacgcacgt accgtcgatg tatctctctg aactaccagg aaaaaaccac
    15421 ggttgtcgcc agggtgcgat cgacgggcat aaagagatca gcaagcgttg ccgggaaatg
    15481 ggcgtcgata ccattatcgt tttcgatact cactggctgg tcaacagtgc ttatcacatc
    15541 aactgtgcag accattttga aggcgtctac accagcaacg agctgccgca ttttattcgc
    15601 gacatgacct acaactacga gggcaacccg gagttggggc agctaattgc cgaggaagcc
    15661 ttaaagctcg gcgtgcgggc aaaagcgcac aatattccca gcctgaaact ggaatacggc
    15721 acgctggtac cgatgcgcta catgaatgaa gacaagcact tcaaagtggt ctccatttcg
    15781 gctttctgca cggttcacga ttttgccgac agccgcaagc tgggcgaagc gattctgaaa
    15841 gcgatcgaac agtacgacgg caccgtggcg gtccttgcca gcggttcgtt atcgcaccgc
    15901 tttattgacg atcagcgtgc ggaagaaggg atgaacagct acacccgcga gttcgaccgc
    15961 cagatggacg agcgtgtggt gaagctgtgg cgcgaaggcc agttcaaaga gttttgcaat
    16021 atgctgccgg agtacgccga ctactgctac ggcgaaggca atatgcacga cacggtgatg
    16081 ctgctgggga tgctcggctg ggataaatac gacggcaagg tggagtttat taccgagctg
    16141 ttcccaagct ctggcaccgg tcaggttaac gctgttttcc cgctgcccgc gtaaggacgt
    16201 tttatgccgc actttatcgt ggaatgtact gaaaatattc gtgaagaagc ccgcttacct
    16261 gagctgtttg ccagtgttaa cactgcactg gcggcaacgg ggatttttcc gcttggggga
    16321 atacgtagcc gggcgcactg gatcgatacc tggcaaatgg ctgatggcaa gcacgattac
    16381 gcctttgtgc atatgacgct aaaaatcggc agcggacgca gtctggaaag ccgtcaggaa
    16441 gtgggcgaaa tgctgtttga tctgattaaa acgcacttcg cctcactgat ggagagccgt
    16501 tacctagcgc tttcatttga gattgcagag ctgcacccga cgttgaattt caaacagaac
    16561 aacgtacacg cgttgtttaa ataaatttgc accagcttcc aggccggatg ataaggcgct
    16621 gccgcatccg gcaatgccac aggatatcgc tatgttcgat aaacacaccc acaccctgat
    16681 cgcccagcgt ctggatcagg cagaaaaaca gcgcgaacag atccgcgcga tctcgctgga
    16741 ttacccggag atcaccatcg aagacgccta cgccgtgcag cgcgaatggg ttcgcctgaa
    16801 aatcgccgaa agtcgcacgc tgaaaggcca caaaatcggt ctgacctcga aagcgatgca
    16861 ggcaagctcg cagatcagcg aaccggatta cggtgcactg ctggacgaca tgttcttcca
    16921 cgatggcagc gatatcccga ccgatcgctt tatcgtgccg cgcattgaag tggagctggc
    16981 ttttgtgctg gcaaaaccgc tgcgtgggcc aaactgcacg ctgttcgacg tttacaacgc
    17041 cacggactat gtgatcccgg cgctggagct gatcgacgct cgctgccaca acatcgatcc
    17101 ggaaacccag cgcccgcgca aagtattcga caccatttcc gataacgccg ccaacgccgg
    17161 agtgatcctc ggaggtcgtc cgatcaaacc agacgaactg gatctgcgtt ggatctccgc
    17221 cctgatgtat cgcaatggcg tgattgaaga aaccggcgtc gccgctggtg tgctgaatca
    17281 tccggcaaac ggcgtggcct ggctggcgaa caaactcgca ccgtatgacg tacaactgga
    17341 aaccgggcaa atcattctcg gtggttcgtt caccctcccg gttccggcgc gtaagggcga
    17401 caccttccac gtcgattacg gcaacatggg ctccattagc tgccgctttg tttaaggaga
    17461 gaacgatgga aaacagtttt aaagcggcgc tgaaagcagg ccgtccgcag attggattat
    17521 ggctggggct gagcagcagc tacagcgcgg agttactggc cggaccagga ttcgactggt
    17581 tattgatcga cggtgagcac gcgccgaata acgtgcaaac cgtgctcacc caactacagg
    17641 cgattgcgcc ctaccccagc cagccagtgg tacgcccatc gtggaacgat ccggtgcaaa
    17701 tcaaacaact gctggacgta ggcacacaaa ccttactggt gccgatggta caaaacgccg
    17761 acgaagcccg taaagcggta cgcgccaccc gttatccccc cgccggtatt cgcggtgtgg
    17821 gcagtgcgct ggctcgcgcc tcgcgctgga atcgcattcc tgattacctg caaaaagcca
    17881 acgatcaaat gtgcgtgctg gtgcagatcg aaacgcgtga ggcaatgaag aacttaccgc
    17941 agattctgga cgtggaaggg gtcgacggcg tgtttatcgg cccggcggat ctgagcgccg
    18001 atatgggtta tgccggtaat ccgcagcacc cggaagtaca ggccgccatt gagcaggcga
    18061 tcgtgcagat ccgcgaagcg ggcaaagcgc cggggatcct gatcgccaat gagcaactgg
    18121 caaaacgcta tctggaactg ggcgcgctgt ttgtcgccgt cggcgttgac accaccctgc
    18181 tcgcccgcgc cgccgaagcg ctggcagcac ggtttggtaa tagttcttct atttcgatgg
    18241 cgaagcaaaa caacaactct gtctactaaa cgtacgatgt ggagcgcatc atgacgacct
    18301 ctaccctgca atataaagat aataaaactg ttgaaattga acatcgtgtc attaataaat
    18361 tatttcgtcg cctaatcatt tttcttttta ttctgtttgt tttttcgttt cttgatcgca
    18421 tcaatatagg atttgccgga ctgacaatgg gcaaggatct tggcctgacg tccacaatgt
    18481 ttggcctcgc cgcaacactc ttttatgtca cgtacgtcat gtgcgggatc cccagtaata
    18541 tgatgttagc gaaaattggc gctcgtcgat ggatcgctgg gatcatggtg gtctggggaa
    18601 ttgcttccac ctgcacaatg tttgcaacaa gccccgaaac actctatcta ctgcgtatgc
    18661 tggttggtat tgctgaggcg ggttttttac ctggtatttt ggtttatctg acctggtggt
    18721 tcccggcata tcatcgggcc agagccaatg ccttatttat gatagcgatg ccagtcacta
    18781 tgatgctggg attgatcctt tcaggctaca ttctggcctt ggatggactg tggaatctaa
    18841 aaggctggca atggctcttt ttgctggaag gtctgccatc ggtcattctt ggtgtcgtca
    18901 cctggtttta tctcaacgac acaccagacc aggcgacctg gcttgatgaa aacgaaaaac
    18961 aggctttaaa aacgatgatt ctccgtgaac aagagagtgc atttgcccat gcatctgttc
    19021 cacgctcaac cttacgtgaa gtcctgaccc cagccatcat gctatataca cttgcttatt
    19081 tttgcccgac taatactctg agtgcgatta atatctggac gccgcaaatc ctacagagtt
    19141 tcaatactgg cagtagcaac atcatgattg gtttgctggc ggccatcccc caattctgca
    19201 caatttttgg catgatctgg tggagtcgtc gttccgaccg tcttaaagaa cgcaaaaagc
    19261 atacgattct tccctacctg tttgccgctg ccggatggtt actcgcttct gcgacagatc
    19321 acagcctcat tcaactgagc agcatcatta tggcctcaat gggatcattt accgcgatga
    19381 ctatcttttg gacaacaccg gatcaagtta taagcttgca atcgcgtgcc gttgcgatgg
    19441 ctgtcatcaa tgctgtcggc aatgtaggtt cagcagtcag tccattactt atcggtattt
    19501 tgaaagatgc aaccggcagt tttagctcag gtttgtggtt tgtagcagga ttgctggtac
    19561 tgggcgcgtt agtcatgacc cgaatcccta tggggagtcg ggaacactct ctttcatctg
    19621 aacttatcgc accaaaaccg caataaggag tcatgatgtc cagtgaagct atctttgaac
    19681 atggttatga aaatattgat ttggtccggg aatatgatgc ccgctacgct ggtgaagatg
    19741 tgcattatga aacatttgcc cggttagcga atttctttgg acgcgatatg cgccctcact
    19801 ggcacgatag atattttcaa ctccattatc tggtcactgg acgcattact ttacaactgg
    19861 atgagcactt ctattctgtg cgcgcaccgc tttttgtact aacaccacca tcggtgccac
    19921 atactttctt tactgatcct gatagcgatg ggcatgttct gaccgtacgt caggagttaa
    19981 tttggcccct ggtagaaaag ctctggcctg gtagtggaga tgccgcaatg ctacaaggta
    20041 tctgtctgtc actggaggga atggaacata cactggcggc attaaatcat tattggccga
    20101 taatatccct ggagtttcaa caaaataagc aaggacggga aattctattg caatcactgg
    20161 cgcaaagtat ttttacaatc ctactgcgtg aagcaccacc aaatgatatt tcaacatgca
    20221 gcgtgcgcgg cgagatgtgt ttattccaga agtttaatcg gctgattgac gaaaattatc
    20281 gccaacattt aatggtccct gaatatgcaa caatgctcgg tatgagtgaa tcacggctta
    20341 ccgagctgtg ccgacgtttt gccaatcaat cgccgaagag attgattttc gaacgagtga
    20401 gccgggaagc cagacggctt ctgctctata gcgagcaaag catcaaccag atagcccttg
    20461 atcttggcta caaagatccc gcctatttcg cccgtttttt caaccgaatg atgggttgct
    20521 caccaacaca attccgtggt cgctgaccat ttatcaacct gagagactcg acatgaaaat
    20581 atctttacgc tcactatttg tggcgtgcct gattgcgcca gtggcattca cctacgcctc
    20641 cgaaccgcaa taaatgaaaa ttgtactact tggaacgaaa ggtggccctt ctttattaaa
    20701 agctgaccgt ttaccgatgg caacggcact tattattggc aataaaacct ggttaattga
    20761 tgctggttac ggggccagct tacaactggt aaaaaataat attccactgc gccagattga
    20821 taatatttta ctcacccatt tacacagtga tcatattctg gactatccat cagtcttaat
    20881 gaatgcatgg gctagcggtc tgcaggacaa aactattcat gtattcggtc cgccaggcac
    20941 ccgagccatg accgctgcca gttggcaggt cttcgatcgg gacatcacac tgcgtatgga
    21001 agaagaagga aaacccgatc ctcgcaaact cgttacagct caagatgttg gtgaaggcgt
    21061 agtttataaa gatgataccg tgaccgtgac tgcattaaag gtccctcatt ctcctttccc
    21121 tgacggtgag gcttttgctt atcgcttcga cacacaaggc aagcgaattg tcttctcagg
    21181 agatacctcg tactttcccc cactggcaac tttctcagaa ggcgctgata tacttgtgca
    21241 tgaggccgta catgagccct ccgtcgctaa gctggcagac agtattggca atggtaaaac
    21301 actggcaaaa gccattgctt ctcaccatac gactattgag gatgtaggca aaattgccaa
    21361 tgaagcaaaa gtgaaaaaac tcatccttag ccacctggta ccaatcaccg ttccggatga
    21421 aacatggaag caagaagcac agaaaactat tcaggtccta tcgtcgttgg tcatgaaaat
    21481 atgacgatta ccattccatt cgccccataa aaaattccgt ccccgttagc ccttgttagc
    21541 ggggaatatt atgatttgcc ttactgtaag ttgatcacga tcgcagttta ctatttaact
    21601 ccaccttgct gtcgaaaagt accaacgttc gcctgaaacg tctcttcccg acaaccgcgt
    21661 tcaacgcttc aattaaataa caaaaacaac acacaaaatt aacaatcgta ttccgattaa
    21721 tactgtagag gtcgacatga aaccagaaga tttccgcgcc agtacccaac gtcctttcac
    21781 cggggaagag tatctgaaaa gcctgcagga tggtcgcgag atctatatct atggcgagcg
    21841 agtgaaagac gtcaccactc atccggcatt tcgtaatgcg gcagcgtctg ttgcccaact
    21901 gtacgacgcg ctgcacaaac cggagatgca ggactctctg tgttggaaca ccgacaccgg
    21961 cagcggcggc tatacccata aattcttccg cgtggcgaaa agtgccgacg acctgcgcca
    22021 gcaacgcgac gccatcgctg aatggtcacg cctgagctat ggctggatgg gccgtacccc
    22081 agactacaaa gccgctttcg gttgcgcact gggcgcgaat ccgggctttt acggtcagtt
    22141 cgagcagaac gcccgtaact ggtatacccg tattcaggaa actggcctct actttaacca
    22201 cgcgattgtt aacccaccga tcgtcatttg ccgaccgata aagtgaaaga cgtttacatc
    22261 aagctggaaa aagagactga cgccgggatt atcgtcagcg gtgcgaaagt ggttgccacc
    22321 aactcggcgc tgactcacta caacatgatt ggcttcggct cggcacaagt gatgggcgaa
    22381 aacccggact tcgcactgat gttcgttgcg ccaatggatg ccgatggcgt gaaattaatc
    22441 tcccgcgcct cttatgagat ggtcgcgggt gctaccggct cgccatacga ctacccgctc
    22501 tccagccgct tcgatgagaa cgatgcgatt ctggtgatgg ataacgtact gatcccatgg
    22561 gaaaacgtgc tgatctaccg cgattttgat cgctgccgtc gctggacgat ggaaggcggt
    22621 ttcgcccgta tgtatccgct gcaagcctgt gtgcgcctgg cagtgaaact cgacttcatt
    22681 acggcactgc tgaaaaaatc actcgaatgt accggcaccc tggagttccg tggtgtgcag
    22741 gccgatctcg gtgaagtggt ggcgtggcgc aacaccttct gggcattgag tgactcgatg
    22801 tgttcagaag cgacgccgtg ggtcaacggg gcttatttac cggatcatgc cgcactgcaa
    22861 acctatcgcg tactggcacc aatggcctac gcgaagatca aaaacattat agaacgcaac
    22921 gttaccagtg gcctgatcta cctcccttcc agtgcccgtg acctgaacaa tccgcagatc
    22981 gaccagtatc tggcgaagta tgtgcgcggt tcgaacggta tggatcacgt ccagcgcatc
    23041 aagatcctca aactgatgtg ggacgccatt ggcagcgaat ttggtggtcg tcacgagctg
    23101 tatgaaatca actactccgg tagccaggat gagattcgcc tgcaatgtct gcgccaggca
    23161 caaagctccg gcaatatgga caagatgatg gcgatggttg atcgctgcct gtcggaatac
    23221 gaccagaacg gctggactgt gccgcacctg cacaacaacg acgatatcaa catgctggat
    23281 aagctgctga aataacgcag caggaggtta agatgcaatt agatgaacaa cgcctgcgct
    23341 ttcatgacgc gatggccagc ctgtcggcag cggtaaatat tatcaccacc gagggcgacg
    23401 ccggacaatg cgggattacg gcaacggctg tctgctcggt cacggataca ccaccgtcgc
    23461 tgatggtgtg cattaacgcc aacagtgcga tgaacccggt ttttcagggc aacggcaagt
    23521 tgtgcgtcaa cgtcctcaac catgagcagg aactgatggc gcgccacttc gcgggcatga
    23581 caggcatggc gatggaagag cgttttagcc tctcatgctg gcaaaaaggt ccgctggcgc
    23641 agccggtgct aaaaggttcg ctggccagtc ttgaaggaga gatccgcgat gtgcaggcga
    23701 ttggcacaca tctggtgtat ctggtggaga ttaaaaacat catcctcagt gcagaaggtc
    23761 acggacttat ttactttaaa cgccgtttcc atccggtgat gctggaaatg gaagctgcga
    23821 tttaagctcc agccccttca cgcattcgtc gaaggggcat ttttattgtg atctcaatca
    23881 catatctacc cgatgaataa ttcttgccca accgctcacc gccattcctg accgcttaca
    23941 tccctaaaat aaccactcag ttatttacct tactttacgc gcacgtatct ctggcaacat
    24001 cactgcagga tagcggtcaa tttacctcct caaacgcaac gcaaacctag aacggcttcg
    24061 gcctatgatt aacaaattac tctatggatt ttggtttgca ggaaggcagc aagtgagtga
    24121 atccccggga gcttacaaaa gtaagtgtca gaggtgaacg aacgcagctg ccgcacctgt
    24181 aagccaaaag acgacgagta aaatgccagg ttttactatg gatacgaaaa aactattcaa
    24241 gcacataccc tgggtgattc tcggaatcat cggtgcattc tgcctcgcgg tagttgcatt
    24301 acgtcggggg gagcacgtca gcgccctgtg gatcgtggtc gcctctgtgt cggtgtatct
    24361 ggtggcgtat cgctactaca gtctgtacat cgcccagaag gtgatgaaac tcgaccccac
    24421 gcgcgcgacg cctgcggtta ttaacaacga cggtctgaac tacgttccga ccaaccgtta
    24481 cgtgttgttt ggtcaccact tcgccgctat cgccggtgct ggtccgctgg tgggaccggt
    24541 tctcgccgcg cagatgggct acctgcctgg cacgctgtgg ctgctggcgg gggtagtgct
    24601 ggccggtgcg gtacaggact ttatggtgct gtttatctcc tctcgccgta acggcgcatc
    24661 tctcggtgag atgatcaaag aagagatggg gccagtaccg gggactatcg cgctgtttgg
    24721 ctgtttctta atcatgatca tcatcctcgc cgttctggcg ctgattgtgg ttaaagccct
    24781 ggccgaaagt ccgtggggtg tcttcaccgt ttgctcaacc gtaccgattg cgctgtttat
    24841 gggtatctac atgcgcttta tccgtccggg gcgtgtgggt gaagtctctg ttattggtat
    24901 cgtgctgctg gttgcctcta tctacttcgg tggcgtgatt gctcacgatc cgtactgggg
    24961 tccggcactg acctttaaag acaccaccat taccttcgcg ctgattggct atgcgtttgt
    25021 ttccgcactg ctgccggtgt ggctgatcct cgcaccgcgc gactatctgg caaccttcct
    25081 gaaaatcggc gttatcgtcg gcctggcgct gggtatcgtg gtgctgaacc cggaactgaa
    25141 aatgcctgcg atgacccagt acattgacgg tactggcccg ctgtggaaag gcgctctgtt
    25201 cccgttcctg ttcatcacca tcgcctgtgg tgcggtatct ggcttccacg cgctgatctc
    25261 ttccggtacg acgccgaaac tgctggctaa cgaaaccgac gcgcgtttca ttggctacgg
    25321 cgcaatgctg atggagtcct tcgtggcgat tatggcgctg gttgcagcgt ccatcatcga
    25381 accgggtctt tacttcgcga tgaacacccc gcctgctggc cttggcatca ccatgcctaa
    25441 cctgcatgaa atgggtggcg agaacgcgcc gatcatcatg gcgcagctga aagacgttac
    25501 cgcacacgcg gcagcgaccg tcagctcctg gggcttcgtg atttcgccag agcagatcct
    25561 gcaaaccgcg aaagacattg gtgagccttc tgtcctgaac cgtgcaggtg gtgcgccgac
    25621 gctggcggta ggtatcgctc acgtgttcca caaagtgctg ccgatggctg acatgggctt
    25681 ctggtatcac ttcggtattc tgttcgaagc cctgttcatc ctgaccgcgc tggatgcggg
    25741 tacccgttct ggccgcttta tgctgcaaga cctgctgggt aacttcatcc cgttcctgaa
    25801 aaaaaccgac tctctggttg ccggtatcat cggtactgcg ggctgtgtgg gtctgtgggg
    25861 ctacctgctg tatcagggcg tggtcgatcc gctgggcggc gttaagagcc tgtggccgct
    25921 gttcggtatc tccaaccaga tgctggcagc cgtagcgctg gtactgggca ccgttgtgct
    25981 gattaagatg aagcgcaccc aatacatctg ggtaactgtt gttccggctg tatggctgct
    26041 tatctgcacc acctgggcgc tgggtctgaa actgttcagc accaacccgc agatggaagg
    26101 cttcttctac atggcaagcc agtacaaaga gaagattgct aacggtactg acctgacggc
    26161 gcagcagatt gccaacatga accacatcgt tgtgaacaac tacaccaacg caggcctgag
    26221 tattctgttc ctgattgtgg tgtacagcat catcttctac ggtttcaaaa cctggcttgc
    26281 ggtgcgtaac agcgacaaac gtactgacaa agaaacaccg tacgttccaa tcccggaagg
    26341 cggcgtgaag atctcttcgc accactaacc gtgtttagcc ccgcttcggc ggggctttgt
    26401 tcttatcaga gtgaactatg tttggtaact taggacaggc aaaaaaatat ctcggccagg
    26461 cggcgaagat gttgattggt attccagact acgacaacta cgtcgagcat atgaagacca
    26521 accatcccga caagccgtac atgagctatg aagaattctt ccgcgaacgc cagaacgcgc
    26581 gctacggcgg cgatggtaaa ggcggtatgc gctgttgtta atggagagag catgaacccg
    26641 attgcagtta ccctactcac cggtttttta ggcgcgggca aaactaccct gctgcgccat
    26701 attcttaacg aacaacacgg ctacaagatt gccgtgattg aaaacgaatt cggcgaagtc
    26761 tccgttgatg accaactgat tggcgaccgc gccacgcaga tcaaaacgct gaccaacggc
    26821 tgcatctgct gttcgcgctc caacgagctg gaggacgcac tactcgacct gctggataat
    26881 ctcgacaagg gcaatattca gttcgatcgt ctggtgattg aatgcaccgg catggccgat
    26941 cccggcccga ttattcagac ctttttctcc catgagattt tatgccagct ctacctgctg
    27001 gacggcgtga ttgcgctggt ggatgcggta catgccgatg agcagatgaa ccagttcacc
    27061 atcgcccagt cccaggtggg ctacgccgac cgtattctgc tgactaaaac cgacgtcgca
    27121 ggcgaagcag agaaactgcg tgaacgcctg gcgcgcatca acgcccgcgc accggtctac
    27181 accgtcaccc acggcgacat cgacctgggc ctgctgttca acaccaacgg ttttatgctg
    27241 gaagagaacg tcgtcagcac caaaccgcgt ttccacttta tcgcggataa acaaaacgat
    27301 atttcgtcaa ttgtggtgga actggattac ccggtagata tcagcgaagt ttcccgcgtg
    27361 atggaaaacc tgctgctgga atcggcggat aaactgctgc gttacaaagg gatgctgtgg
    27421 attgacggcg aacctaaccg cctgctgttc cagggcgtcc agcgcctcta cagcgccgac
    27481 tgggacaggc cgtggggcga tgaaaaaccg catagcacaa tggtgtttat cgggattcag
    27541 ttgccggagg aagagattcg ggcggcgttt gcgggattga ggaagtaagt tcgggaaaga
    27601 tcaacttttt gagagttcca ccgcaacatt attacgagtg gaactcttta gatatgtcag
    27661 accaaccaaa gcttcttcaa ttgcgttttc attttttcat gtctcatttt cagttcagcc
    27721 aatccccatt ctgaagaatt acaaacatct tttgttaaat aaaaagagct tttactatta
    27781 agtttctcat aaatagattt ctttttgata aaatcatagt gttgtgtttc agaatttttg
    27841 gaaccactta tgagtgttaa attacctatt ttatgcagcc agtagacatg ctcttgaggt
    27901 tggaatctat taatccagta ttcattaact aatgcttgag gaagaatatg ctcaattgtg
    27961 attctccccg tataggtttt aatcacgctc tcatcttgtt gctccatatc taagcgaagc
    28021 aaaattgctt ttattaagtt cacttgattc ggacgaggct catataaatc ttcatccaga
    28081 gcagctataa aacctgaatt atctgcatgt tggtttatct gatttatcac agaatcaaaa
    28141 ggcatatcat tatttattgc taccagagca gaataacaaa ccatttctct ttggctttta
    28201 atttgttttt ttaaccaccc atgcatatat accttctcaa aggcggtaat aaattgagaa
    28261 aaatcatcta aattaaaatc ttctgtacga gccattcgat tcatgaatgc cataaccgga
    28321 ggaatccact catctacccc ta
//
LOCUS       DAJTQQ010000049        28170 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000049 DAJTQQ010000000
VERSION     DAJTQQ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28170
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.049"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(31..1026)
                     /gene="yjfF"
                     /locus_tag="OO309_002285"
                     /pseudo
     CDS             complement(31..1026)
                     /gene="yjfF"
                     /locus_tag="OO309_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(1013..2035)
                     /locus_tag="OO309_002286"
     CDS             complement(1013..2035)
                     /locus_tag="OO309_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5847406.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2049..3551)
                     /locus_tag="OO309_002287"
     CDS             complement(2049..3551)
                     /locus_tag="OO309_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5847407.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3691..4647)
                     /gene="ytfQ"
                     /locus_tag="OO309_002288"
                     /pseudo
     CDS             complement(3691..4647)
                     /gene="ytfQ"
                     /locus_tag="OO309_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4957..5487
                     /gene="ppa"
                     /locus_tag="OO309_002289"
     CDS             4957..5487
                     /gene="ppa"
                     /locus_tag="OO309_002289"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR5847408.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5867..6208)
                     /locus_tag="OO309_002290"
     CDS             complement(5867..6208)
                     /locus_tag="OO309_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR5847409.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6211..9990)
                     /gene="tamB"
                     /locus_tag="OO309_002291"
     CDS             complement(6211..9990)
                     /gene="tamB"
                     /locus_tag="OO309_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR5847410.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9987..11720)
                     /gene="tamA"
                     /locus_tag="OO309_002292"
     CDS             complement(9987..11720)
                     /gene="tamA"
                     /locus_tag="OO309_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR5847411.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11926..12564
                     /gene="msrA"
                     /locus_tag="OO309_002293"
     CDS             11926..12564
                     /gene="msrA"
                     /locus_tag="OO309_002293"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR5847412.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12887..14230
                     /locus_tag="OO309_002294"
     CDS             12887..14230
                     /locus_tag="OO309_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5847413.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14292..14498)
                     /locus_tag="OO309_002295"
     CDS             complement(14292..14498)
                     /locus_tag="OO309_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR5847414.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14823..15377
                     /locus_tag="OO309_002296"
     CDS             14823..15377
                     /locus_tag="OO309_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5847415.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15440..16378)
                     /locus_tag="OO309_002297"
     CDS             complement(15440..16378)
                     /locus_tag="OO309_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5847416.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16590..17330)
                     /gene="cysQ"
                     /locus_tag="OO309_002298"
     CDS             complement(16590..17330)
                     /gene="cysQ"
                     /locus_tag="OO309_002298"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR5847417.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17520..19463
                     /gene="cpdB"
                     /locus_tag="OO309_002299"
     CDS             17520..19463
                     /gene="cpdB"
                     /locus_tag="OO309_002299"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR5847418.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19589..19969)
                     /locus_tag="OO309_002300"
     CDS             complement(19589..19969)
                     /locus_tag="OO309_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5847419.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20058..20918
                     /gene="qorB"
                     /locus_tag="OO309_002301"
     CDS             20058..20918
                     /gene="qorB"
                     /locus_tag="OO309_002301"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5847420.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21027..21992
                     /locus_tag="OO309_002302"
     CDS             21027..21992
                     /locus_tag="OO309_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5847421.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22100..22762
                     /gene="ytfE"
                     /locus_tag="OO309_002303"
     CDS             22100..22762
                     /gene="ytfE"
                     /locus_tag="OO309_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR5847422.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22807..24219)
                     /gene="cycA"
                     /locus_tag="OO309_002304"
                     /gene_synonym="dagA"
     CDS             complement(22807..24219)
                     /gene="cycA"
                     /locus_tag="OO309_002304"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR5847423.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24527..25147)
                     /gene="fklB"
                     /locus_tag="OO309_002305"
     CDS             complement(24527..25147)
                     /gene="fklB"
                     /locus_tag="OO309_002305"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5847424.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25366..25911
                     /locus_tag="OO309_002306"
     CDS             25366..25911
                     /locus_tag="OO309_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HCR5847425.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25895..26542)
                     /locus_tag="OO309_002307"
     CDS             complement(25895..26542)
                     /locus_tag="OO309_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5847426.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26754..27545
                     /locus_tag="OO309_002308"
     CDS             26754..27545
                     /locus_tag="OO309_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCR5847427.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27555..>28170
                     /locus_tag="OO309_002309"
     CDS             27555..>28170
                     /locus_tag="OO309_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR5847428.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKR"
BASE COUNT         6915 a         7508 c         6944 g         6803 t
ORIGIN      
        1 atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga ctcttgtcac
       61 tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg ctataaaaat
      121 aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc cgtcaaagtt
      181 gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa gcaccgtccc
      241 gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt ccagttccac
      301 acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa tccccgccag
      361 tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag tagaaatccc
      421 catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc caaaacgggt
      481 acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc ccatcgcact
      541 aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat aaatgggatg
      601 gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa agaacatccc
      661 cgcaagcgta atgataaatg ccgggatctt cagggcgtcg atcagaagcc ccataaatgc
      721 gccgaaggca cagcccatca ccagcactag cggaaacgcc agcagcggcg agaggccgaa
      781 atcgccaacc acttttgcca gaaacacgcc agtaaaggcg atcaccgaac cgacggagag
      841 atcgataccg ccagagagga tcacaaaggt catgccaacg gcgattatcc caagaaaggc
      901 gttatcggtc aggatattgc agatcaccct tgtggaagca aaaccgggaa actgggtcag
      961 gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagcggca agttacgttt
     1021 tatcatggct acgtactcct ttaatcagac tgataaagcg ttgcgactga acaatcagca
     1081 cacaaagcac caccaccgct ttcaccacct ggttcatctc cggcggaaag cccgaaagca
     1141 aaattccggt gttcatcccc tgaataatca gcgcccccac taccgagagc agcaggttaa
     1201 agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg tccagctcca
     1261 gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg ataatgcccg
     1321 caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt cgcgtgttta
     1381 ccccggcatt ttttgccgcc cgaatgttga taccaacgac ttcgataaac atccccagcg
     1441 ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata atgaccggcg
     1501 ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag ttaaacgtga
     1561 cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc aggatcaggg
     1621 tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac aatcccgcca
     1681 ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc gcgaccgtca
     1741 tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga tcgataccac
     1801 cggtggcgat caccagcgta ataccaatcg ccagtaacgc aacgggagct gcacggttaa
     1861 gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc cagaaatgcg
     1921 gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc ggcatccccg
     1981 tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga ggcatcacac
     2041 tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg tgccagcggg
     2101 atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata gcccaccagc
     2161 tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag cgtttcaatc
     2221 aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttagctc atcaaggatc
     2281 agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg attgccaccg
     2341 gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg gcggataaag
     2401 cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc gcgctgggcc
     2461 tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc atcggttttc
     2521 ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga tcgcagggtt
     2581 tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc gaagatcact
     2641 tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat ctcgccgggg
     2701 cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt gaacgcggca
     2761 acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt atccagctcg
     2821 cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc ccgacagcct
     2881 acgaaactgc cgttgcgtaa gacggtgatc cgatcgctga cctgatagac ctgatcgaga
     2941 aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat caggccaaat
     3001 aatagctcca tttcctgggt gtcgaggctg gcggtgggtt catcgaggat cagcactttg
     3061 gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgccac cgaaaaacgg
     3121 ttaagcggtt cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc ggtggcgcgc
     3181 ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc tatgaataga
     3241 ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt gccgataccg
     3301 agttgttgtg catgggcggt atttttcggt gagatagtct ggccttccag ccagatggtg
     3361 ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt tcccgccccg
     3421 ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac gttgtctaac
     3481 gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc ctggtgttgg
     3541 tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga taagacgcgg
     3601 taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg ccttatccgg
     3661 cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct tcttctctaa
     3721 ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca gcttttcagg
     3781 catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg ccatgttcgg
     3841 cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga tgtccggtac
     3901 accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt ctttaattgc
     3961 ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga tgtttttgcc
     4021 gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc gggtgaagtc
     4081 acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt cggcaaagcc
     4141 tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca ccacgttgca
     4201 tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt cgaggatgtt
     4261 gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg aacggtcgag
     4321 caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc aacccgtcgc
     4381 aaccactgga gcgataaaga tcgcatccac cccttatgca acgaaggaac gtaccgcttt
     4441 aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc cgcgtttttc
     4501 ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg atccgacctg
     4561 cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg ccgagactgc
     4621 agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat tattattggt
     4681 gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg cgttacatca
     4741 cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc atctcactat
     4801 gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa aactggcgaa
     4861 aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct ataatacttg
     4921 gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa cgtccctgcg
     4981 ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa cgcagatccg
     5041 atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt catgtccacc
     5101 gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct ggacggtgac
     5161 ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt gatccgttgc
     5221 cgtccggttg ccgttctgaa aatgaccgac gaagccggtg aagatgcgaa actggttgcg
     5281 gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa cgatctgcct
     5341 gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct cgaaaaaggc
     5401 aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga aatcgttgct
     5461 tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg aacgccgggc
     5521 ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc caccatcatt
     5581 attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct
     5641 gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt caaagcgtgc
     5701 ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg attgcagata
     5761 tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga
     5821 tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac ttatccctgt
     5881 ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga taaacgtaca
     5941 tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc
     6001 gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtaccg
     6061 ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga
     6121 tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc
     6181 gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag
     6241 tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa
     6301 cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa
     6361 tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt cgagcgctaa
     6421 attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacccc
     6481 cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct ccagcccttg
     6541 tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc
     6601 cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat cgtcttctgt
     6661 agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg gaccagagaa
     6721 cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg
     6781 gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt taagatcgcc
     6841 cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat
     6901 caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt
     6961 aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt gcaccacgat
     7021 gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa
     7081 tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc gcactttact
     7141 gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt
     7201 caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc gcataccgtt
     7261 aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc cgtcgatatc
     7321 cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg
     7381 caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat
     7441 cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc
     7501 ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg tccagcccaa
     7561 ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg ccaccggcag
     7621 cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg tgatactgcc
     7681 ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg tgaagatacc
     7741 gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga agcggttgag
     7801 attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc acagttccgc
     7861 attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat tgcggtaatc
     7921 cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc gggtattgct
     7981 aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat taagctgccc
     8041 ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc
     8101 attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc
     8161 gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgccac
     8221 ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc
     8281 caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt
     8341 atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcgcc
     8401 tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca tccactgcat
     8461 ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt
     8521 cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt
     8581 aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt taatgccatt
     8641 aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttgagttc
     8701 agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct gttcattacc
     8761 tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg ccgtacgcat
     8821 agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat ccgcctgata
     8881 ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagtgg
     8941 caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag
     9001 gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct tcaccttttc
     9061 acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg gccagttgtc
     9121 tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga tatccagggc
     9181 gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc gcacggtgat
     9241 gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt tcaggttaag
     9301 cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat ctttcagcgt
     9361 ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga ccacttcttc
     9421 ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg acgtcggttt
     9481 cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca ccgacaccgt
     9541 ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg tgatgggata
     9601 cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg
     9661 cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat taatacaaac
     9721 gctactgttc cacaggcact caagaccgac ccccagatgc agattacccg cttttaccgc
     9781 cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac
     9841 cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa gatgcaggcc
     9901 gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa cgataaccac
     9961 gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt
    10021 aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat tggcccgacc
    10081 ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg gcgaatatcg
    10141 cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt cacgttgtac
    10201 tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc gttggcgtat
    10261 ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc gacgaagaaa
    10321 cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt
    10381 gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgtgctgcgc ctggaaaacg
    10441 gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag
    10501 tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga
    10561 taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag gctccagcgc
    10621 aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc caccagcgta
    10681 gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa
    10741 tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag ggtctgttcc
    10801 ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat ccacggcttt
    10861 ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc ggtttcgatg
    10921 gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct
    10981 ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga aagtcggcgg
    11041 ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg caccagattt
    11101 tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt
    11161 tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc
    11221 tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact ggttaaggac
    11281 tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg
    11341 agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg
    11401 ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg ccctttcttt
    11461 ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct
    11521 tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt
    11581 tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa cccctcgacc
    11641 tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat
    11701 aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc cctggaacgc
    11761 cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta aaccccaaat
    11821 atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa tacgcctctt
    11881 gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag tttatttgat
    11941 aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc gatgcccgta
    12001 gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg aatggagatt
    12061 gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca gttacccggc
    12121 gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac ttatcgggaa
    12181 gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga tccttccgtc
    12241 atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc ccagggaatg
    12301 cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct gaccccagaa
    12361 caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct tgccgccgat
    12421 gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta tgccgaagat
    12481 gaccaccagc aatatctgca taaaaaccca tatggttatt gtggaattgg cggaattggc
    12541 gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt gataaagtgc
    12601 tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat cgcccaaatt
    12661 caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca gtattgcatt
    12721 tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc tatactagcc
    12781 actgaaaatg ccggttcact ttcttcgaat cggctttcaa tgtgtatttc acacaaatta
    12841 atcaactttc ccttccgagg gtctggcctg aaaggtcgga taagatatgt taaacagtat
    12901 tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct
    12961 tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata taaatgccca
    13021 acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc aaatcggtct
    13081 gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc cagcttttca
    13141 cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct ttattctctc
    13201 tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga aacgcatcgg
    13261 tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct tctgcctgta
    13321 cgtttgcacc ccgctggtgt ggttcttcaa cggcctggcg aacatgatct tccgtatttt
    13381 caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg cggtagtgga
    13441 agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg aaaacgtctt
    13501 tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg tgatttggtt
    13561 tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc cgcactctaa
    13621 gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt ctaaagacct
    13681 gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac aaattcgcaa
    13741 cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc
    13801 aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga tcatcaccct
    13861 caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg aagagcagat
    13921 tgtcgcccgt gatgagaact catggctgat tgacggcggc accccaattg acgacgtcat
    13981 gcgcgtgctg gatattgacg agttcccgca gtcgggcaac tacgaaacca tcggcggctt
    14041 tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat tcgccggcta
    14101 caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg tgacccggat
    14161 cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata aagaagaaag
    14221 cgtcgcgtaa cccccagaaa catcaacggc tcctgaatca ggagccgtct tattactgca
    14281 tagcactttg gttaagccat ctctgtttgc agacgcataa cctgacggtt aacttcggac
    14341 atcactgaca aatgcagctt atccttcact tttgggataa gaatcttacc cttatcaaat
    14401 tcaaaagcgc caatgtcctt gatgtataac cgtccacgga acaggatctt tacgtacttc
    14461 gccacctgaa gtgggttgta ccgttggaaa attttcattg ttatctcctg ctgagtatta
    14521 cgcctgtgcg gtgccacaat cggcccaact attatgaggc gcaaatttta atgcttagtg
    14581 actatagact atccgggcaa tgtttccacc gcgtataact ttttttacct aaaggttaca
    14641 attattcaga attatctttt taccgaagcg cggtcttcag tataagcatt catttttcat
    14701 atgatttgtg cgcttgaccg caaactggca tcacacttgc gggaaattcg ataaatagca
    14761 catatgatta aaactcagac ccaagtggtc ggatcacctg catatcataa gaaggaaaca
    14821 ccatgaccct acgcaagatt ctggcactca cctgcctgct gttgccgatg atggcttccg
    14881 cacatcagtt cgaaatcggt cagcgagtgc cgccgattgg cattaccgat cggggcgagt
    14941 tggtgcttga taaagatcag tttagctaca aaacctggaa cagcgcgcag ttagtgggaa
    15001 aagtgcgagt actgcaacat attgctggtc gcacctctgc aaaagagaaa aacgcgacgc
    15061 tgattgaagc gattaaatca gcgaagttac cgcacgatcg ttaccagacc accaccattg
    15121 ttaacaccga cgacgcgatt ccgggttccg gcatgtttgt gcgcagcagt ctggagagta
    15181 ataaaaagct ttatccctgg tcgcagttta ttgtcgatag caatggcgtc gcgcgcggtg
    15241 cctggcagct ggatgaagaa agttccgctg tagtggtgct ggataaagac ggtcgcgtgc
    15301 aatgggccaa agacggggcg ctcacaccgg aagaggtgca gcaagtgatg gacctgctgc
    15361 aaaagttgct caaataatgg caataacata aattctggaa aaataatttt cccctatcgt
    15421 tatctatggg ataggggaat taagtctctg agttcttaca ccgaactaat actggtaacg
    15481 ctagttgctc ttttttaaac gcctcaatgg tcgatttata tttctcgcaa atcttttctt
    15541 tggtaatatt actatctgcg gccagattcg cacttactat cgcatccact tgttcggcta
    15601 attcgacttg ttggcaacct gcattaacca gaaaattata aataagagag taatcttcat
    15661 cctccaaaac aacagaggaa ccattacgaa aacgttcatc ccctccatag attttaagcg
    15721 caaagcccaa agattctatg actatggcaa aattatcatc aattattttt tcgtaaatat
    15781 accatccatc gcattcttta attccaccgc ctggtatttg gagttcaaat ttaccccgtt
    15841 ttaatttgct tactacatcc tccaataagg cataaccgaa agatacgtca ccaaacgcct
    15901 ggcgaatata acctttaatc ctttttggta tgtcagactg ttttttctgc aaaatatcac
    15961 aggcatacaa tcttctgttt tgttctaacg gaaactcgcg gtttttgaaa tccatgaatg
    16021 agttatactc atttattgtt atgggaattt cttcgtattc aaacccactg cctcgcaatg
    16081 ttattgtata ttcagcaccg ttatctttaa ggctgaaact gataagaaaa ttatccatgg
    16141 cattaacagt ttcatttttt gatgaaagca cttgataact atttttatca atctgtgttg
    16201 cgcaacgaaa ggactcccgc aaagttaaca gactatcata attcgaaacc caatcttttt
    16261 tcttaaatgc atgctgaata ttattaaaaa aatcgtttga atgtgttttt gtatgcgatg
    16321 actttacaaa taactccacc gcaccaataa aattattgtg tctgatcctg ggtaacatat
    16381 ggctacctta ttttatttgg ttattttatg tcgccattaa atatctcatt atagaagtaa
    16441 actatcaaaa aacgctcatt attaaaatgt aacttactag caattgaatt tttgcacttt
    16501 aaatacaatc tctttttatt tataactttt taggttggga taaatcagga tggcagaatc
    16561 aggaaataca ctgtttctgc catctgaatt tagtaaatgg acactctgaa ccccggattc
    16621 aggaacgact cgcgcggagt gtaatccagc ggtttaccct gccagtcgtg aacgtgcgct
    16681 ccggcagctg cagccacagc atgtccggcg gcggtgtccc aaatattcgt tggtccgaag
    16741 cgcgggtaca gctgcgcctg tccttccgcc accaggcaga atttcagcga agagccgatg
    16801 gacgtggtct gatgttcgcc aagctgttgc agatactctt tcagctccgc atctgcatgg
    16861 gaacggctga taaccaccag cggcgggcgc gcatcgcgga cctgaatcag cttgcgcaca
    16921 ccgcactctt ctttccaggc cttgccttct gccgcgctgt acattacgtt cattaccggc
    16981 gcatacacca cacctaaaat cggtttgcca tggtcaatga gcgcaatgtt aacggtgaat
    17041 tcgccattac gtttaataaa ctctttagta ccatccagcg ggtctaccag ccagtaacgc
    17101 tgccagtgct gacggacttc ccaaccggga ggatcttctt cagaaaggac cggaatatcc
    17161 ggtgccagcg tacgtaaacc gtccatgata acggtgtgag cggcaatatc cgctgccgtt
    17221 accggagaat tgtccgcttt gctgacgacg tccatcggtt tcgtcccgtc gtagacctgc
    17281 ataatggcat cgcctgcatt ccgtgcaagc tggcatactt gatctaacat ttctccacct
    17341 cgtctctgtg agcggtgtta acttattgtt ttacttatac cctatcgtta atgaatgcgc
    17401 caactgtgat agtgtcatca ttttcaaagc gtaaaattgt ggcattcttc actgttctat
    17461 aagtaaggcg tttattctcc cttttctttc gtattcccga tgataaaagg atgtccctga
    17521 tgattaagtt tagcgcaacg ctcctggcca cgctgattgc cgccagtgtg aatgcagcga
    17581 cggtcgatct gcgtatcatg gaaaccactg atctgcatag caacatgatg gatttcgatt
    17641 attacaaaga caccgccacg gaaaaattcg gactggtacg tacggcaagc ctgattaacg
    17701 atgcccgcaa tgaagtgaaa aacagcgtac tggtcgataa cggcgatttg attcagggga
    17761 gtccgctggc cgattacatg tcggcgaaag gattaaaagc aggtgatgtt catccggttt
    17821 ataaggcgct gaatacgctg gattatacgg tcggtacact cggcaatcat gaatttaact
    17881 acggtctgga ttacctgaaa aatgcgctgg cgggagcgaa attcccttat gtaaatgcca
    17941 acgtcattga cgccagaacc aaacagccaa tgtttacacc gtatttaatt aaagataccg
    18001 aagtggtcga taaagacgga aaaaaacaga cgctgaagat tggctatatt ggcgtcgtgc
    18061 cgccgcaaat catgggctgg gataaagcta atttatccgg aaaagtgacg gtgaatgata
    18121 ttaccgaaac cgtgcgcaaa tacgtgcctg aaatgcgcga gaaaggtgcc gatgtcattg
    18181 tcgttctggc gcattccggg ctgtctgccg atccgtataa agtaatggcg gaaaactcag
    18241 tttattacct cagtgaaatt ccgggcgtta acgccattat gtttggccat gctcacgccg
    18301 ttttcccggg taaagatttt gctgatatcg aaggggctga tatcaccaaa ggcacgctga
    18361 atggtgttcc ggcggtaatg ccgggcatgt ggggcgatca tcttggggtg gtcgacttac
    18421 aactcagtaa tgacagcggt aaatggcagg tgacgcaggc gaaagcggaa gcacggccga
    18481 tttacgacat cgccaataaa aaatccctcg cggcggaaga cagcaagctg gtagaaacac
    18541 tcaaagccga tcacgatgcc acacgccagt tcgtcagcaa gccaatcggt aaatctgccg
    18601 acaatatgta tagctatctg gcgctggtgc aggacgatcc gaccgtgcaa gtcgtgaaca
    18661 acgcgcaaaa agcgtatgtc gaacattaca ttcagggcga tccggatctg gcaaaactgc
    18721 cggtgctttc agctgccgca ccgtttaaag ttggtggtcg caaaaatgac ccggcaagct
    18781 atgtggaggt ggaaaaaggc cagttgacct tccgtaatgc cgccgatctt tatctctacc
    18841 ccaatacgct gattgtagtg aaagccagcg gtaaagaggt gaaagagtgg ctggagtgct
    18901 ccgccggaca gtttaaccag attgctccta acagcacgaa accacagtca ctcatcaact
    18961 gggatggttt ccgcacttat aactttgatg ttattgatgg tgtgaattat cagattgatg
    19021 ttacccaacc cgcccgctat gacggcgagt gccagatgat taatgccaat gcagagcgga
    19081 ttaagaacct gacctttaat ggcaagccga ttgatccgaa cgctatgttc ctggttgcca
    19141 ccaataacta tcgcgcttac ggcggcaaat ttgccggtac gggcgacagc catatcgctt
    19201 ttgcttcacc ggatgagaac cgctcggtgc tggcagcgtg gattgctgat gagtcgaaac
    19261 gtgcggggga aattcatccg gcggcagata acaactggcg tttagcaccg atagctgccg
    19321 ataagaaact ggatatccgt ttcgaaactt ccccgtcaga taaagccgca gcgtttatta
    19381 aagagaaagg gcagtatccg atgaataaag tcgcgaccga tgatatcggg tttgcgattt
    19441 atcaggtgga tttgagtaag taaaacactt ctttttcggc ctataaatca accgcatccg
    19501 gcatttattg gcggatgcga tgctgccgta tcttatccac cctacaagcc atgaaccgta
    19561 gaccggataa gcgcagcgca tccggcagtt atgccgcacg ttcatcccgc accgccaaca
    19621 cctcgggcaa attcaactca atccagtccg ccagtgcagc aaccttttcg ctcacctgct
    19681 cgcccagcgg cgtgaggcta tattccacat gcggcggcac caccggatac gcgatacggt
    19741 taagaaaacc atcctgttct aacgcctgta acgactgcgc aagcatcttt tcactcaccc
    19801 caccgatttt gcgccgcagg tcgctaaagc gatgagtacc ttcgcgtagc gccaccagaa
    19861 tcaacacccc ccaacggctg gtgacgtgtt tcaacacctc gcgcgacggg cactgttccg
    19921 caaagagatt accctctttc agttgttgtg acaggctaac ctgactcatt tcatacttac
    19981 ctttttgcac gtacttacta aaagtaagtt taggtgttag cgtatttaaa cacaagacaa
    20041 aacgatggag acttcccatg atcgctatta ctggtgccac tggccaactt ggtcactata
    20101 ttattgaatc cttgatgaaa acggttcctg ccagccaaat agtggctatc gttcgtaatc
    20161 cggcaaaagc ccaggccctg gcagcacaag gcattaccgt gcgtcaggct gactacagcg
    20221 atgaagccgc actgacatct gcacttcagg gagtggaaaa actactgctg atctcttcca
    20281 gcgaagtggg tcaacgtgcc ccgcagcatc gtaatgttat taatgccgca aaggcggctg
    20341 gcgtgaaatt tatcgcttat accagcctgc tacatgcaga tacctccccg ctcggcctcg
    20401 ccgatgagca catcgagacg gagaaaatgc tggctgattc tggcatcgtt tacaccctgc
    20461 tgcgcaacgg ctggtacagc gaaaactacc tcgccagcgc cccggcagca ctggaacacg
    20521 gcgtatttat cggtgcggct ggcgatggca aaatcgcctc agcaacgcgg gcagattatg
    20581 cgacagctgc ggcacgcgtg attagcgaag ccggtcacga aggcaaggtt tacgaactgg
    20641 cgggcgatag tgcctggacg ttgacacagt tagcggcaga gctgaccaaa cagagcggca
    20701 aacaggttac ctatcaaaat ctgagcgaag ccgatttcgc cgcggcactg aaaagcgtcg
    20761 gactgcccga cggactggcg gatatgctgg cggattctga cgttggcgca tcgaaaggtg
    20821 gtctgtttga taacagcaaa acgcttagca aattgattgg ctgcccaacg acaacgttag
    20881 ccgaaagcgt aagccatctt tttaatgtta ataactagtt aattaaagtg gcatcctccc
    20941 gcatcctctc tgataatgac gggatgccgg gagcaatcat gtctgcttcc tgaacgtttc
    21001 ttctgacaga ccaatggatg ccagtaatga ttagcggcgt gctgtacgcc ctgttagcag
    21061 ggttgatgtg ggggcttatt tttgtcgggc cgttgatcgt gccggaatac ccggcgatgt
    21121 tgcagtcgat ggggcgttat ctggcgttag ggttaattgc gctacccatt gcctggctgg
    21181 gacgcgtgcg tctgcgtcag ttggcgcgtc gggactggct taccgccttg atgctcacta
    21241 tgatgggcaa cctcatttat tacttctgcc ttgccagtgc cattcaacgt actggcgcgc
    21301 ctgtttccac gatgattatc ggcaccctgc cggtggtgat tcctgtcttt gccaatctgc
    21361 tttatagcca gcgcgacggc aaactcgcgt ggggaaaact cgccccggca ctgatttgta
    21421 ttggcatcgg cctggcgagt gtgaatattg ctgagttaaa ccacggactc cccgattttg
    21481 actgggcacg ttatacctct ggcatcgtgc tagcgttagt ttccgtggtc tgctgggcat
    21541 ggtatgccct gcgcaacgcc cgctggctgc gggaaaatcc cgacaaacat ccgatgatgt
    21601 gggcgacggc gcaggcgctg gtcacgctgc cggtttctct catcggctat ctcgtcgcct
    21661 gttactggct gaatatacaa acgccggact tctccttacc ttttggcccc cgtccgctgg
    21721 tgtttattag tctgatggtt gcgatagccg tgctttgctc atgggttggc gcactctgct
    21781 ggaacgtcgc cagccagcga ttaccgacag tgattctcgg gccgctgatt gttttcgaaa
    21841 cgctggcagg tttgctgtac acctttttac tccgccagca aatgccgccg ctaatgacgc
    21901 tgagcggtat cgcgctgtta gtgattggcg tggtcatcgc ggtcagagca aaaccagaaa
    21961 aacctttaac tgaatctgtc tcagaaagtt gacatgctgg cagtgagtta aataagcctc
    22021 tgctacgtaa gggttatagc ttttgcctta aaggtgtatt taaaatacat cttatcttat
    22081 taagaatgag gtatcagcta tggcttatcg cgaccaacct ttaggtgaac tggcgctctc
    22141 tatccctcgc gcttcggctc tgtttcgtaa atatgatatg gattactgct gtggcggtaa
    22201 gcagacgctg gcgcgcgcgg cggcacgtaa agaactggat gttgaggtca ttgaagctga
    22261 actggcaaag cttgctgaac aaccgattga gaaagactgg cgtagcgccc cgctggcaga
    22321 aatcattgac catatcatcg tgcgctacca cgatcgtcac cgcgagcagc tgccggagct
    22381 gatcctgcaa gcgactaaag tcgagcgcgt tcacgccgac aaaccgagcg tgccaaaagg
    22441 gctgacaaaa tacctgacca tgctgcatga agagctttcc agccacatga tgaaagaaga
    22501 gcagatcctc ttcccgatga tcaaacaagg catgggcagc caggcaatgg ggccaatcag
    22561 cgtaatggaa agcgagcacg atgaagcggg cgaactgctg gaagtgatca aacacactac
    22621 caataacgtc acaccgccgc cagaagcctg caccacctgg aaagcgatgt ataacggcat
    22681 taatgaactg attgatgacc tgatggatca catcagtctg gaaaacaatg tactgttccc
    22741 acgcgcccta gcgggtgagt gatctaaaag ctggatggca ttgcgccatc cagcatgata
    22801 atgcggttat ttccgcagtt cagcagcccg cttcttacca ataaacagcc agcccaaccc
    22861 cagcgcgata aaccacagcg gggtaaccag cagcgcctgg cgagtgtcat cttccagtgt
    22921 cagcaacacg accacgaaca caaagaacgc catacatacc cagcacatca gcttgccgag
    22981 cggcatcttg tagatcgact tttcatgcag atgaggacgc tgtttgcggt acaccaggta
    23041 agagcaaagg ataatcgtcc agacgaacat aaacagaatc gcggaaacgg ttgtaatcat
    23101 cgtgaacgcg ccaatcacgc taggattcac atacaacatc accacgccgc cgagcagaca
    23161 gatacacgag aaggtcaacc ctttcgcggg tactgcgcgc ttagaaagtt tagcgaacgc
    23221 tttcggcgca acaccttcct gcgccagacc aaacagcata cggctggtag agaagacacc
    23281 gctgttagcg gaagacgccg cagaggtcag caccacaaag ttgatcacgc tggcagccgc
    23341 aggcaaacct accaacacaa acagctcaac gaacgggctt ttctccggga ctaccgaact
    23401 ccacggtgtc acggacataa tcacaatcag cgcgaagacg tagaacataa tgatacggat
    23461 cggaatggag ttaatcgcgc gtggcagtga tttctccgga tctttggttt ccgcagctgt
    23521 tgtacctacc agctcaatcc ccacgaaagc gaaaaccgct atctggaatc cggcaaagaa
    23581 gccacttaaa cctttcggga accagccgcc gtcattccac aaatgcgcga atgacgcttc
    23641 cacaccggtc ggtgactgaa agtgcatcgc caccatgacc aggccgacga caatcagcga
    23701 cacgatggcg acgattttga tcatcgcaaa ccagaactcc atctcaccga acattttcac
    23761 ggtggcaagg ttgagcgtca gcagcagcac tatcaccgcc agcgaggcga cccagtcgga
    23821 gagatcgggg aaccagaact gagcataagc cgtgatcgcc accacgtctg ccataccggt
    23881 tacaacccag cagaaccagt aagtccagcc ggtgaaatat cccgcccacg gccccagtaa
    23941 atcggaagcg aagtcactaa aagatttgta ttccagattc gaaagcagca attcccccat
    24001 tgcccgcatc acgaaaaaga gcataaaacc aatgatcata taaacgaaaa tgatcgacgg
    24061 cccggcaagg ctaatcgttt tgccggaccc cataaacaac ccagtaccaa tggcacctcc
    24121 aatggcaata agctgaacat gtcggtttgt gagattgcgc cgtagcgact gttcagccgg
    24181 agcctgatca tcggcaacga cttttacctg atctaccatg ttttttcttc ctgtacctgt
    24241 ctgtgttgtt caggctctac ggcctttagt cagtctatga tacgacgaat ctgtatcggg
    24301 actcatcgat attaggtaac aatacgcggg atgaatacta agatttagat ctaatgttaa
    24361 ttttatgttt aaagtgagtg tcatatcacg gtgataacgc gaaacagctc acaaaaatac
    24421 acgcttacgg ggtctttgca acacaaaata gcgccgtttg gcaaactgaa gggtttattg
    24481 ctgaatgcct gctcccctct ggttcgaggg gaacagaatg cgctgcttag aggatttcca
    24541 gcagttcgac ttcaaacacc agggtgctga acggagggat ggatgcacct gcgccgcgct
    24601 cgccatatgc cagttcctgc gggatagtca gttcccattt agaacctacc ggcatcagag
    24661 tcagtgcttc aatccagcca gggatcacgc cattaaccgg gaattcagcg ggttcaccac
    24721 gagcaacgga gctgtcaaac acggtgccgt cgatcagttt accggtgtaa tgaacacgaa
    24781 cgcggtcggt acgtgccgga attgcgcctt caccctggtt gatcacgcgg aattgcaggc
    24841 cagattcggt gctattcaca ccttcttttt tggcgttttc ttccaggtat ttcacgcctt
    24901 cagcagccat cgcctggaaa cgctgacgac gaacggcatc ggcgcgttcg tggatttcac
    24961 gcagcgcgcg atgcaccaca tcaaccggca cagccggatg tttgccttcc agcgcatcgg
    25021 caatacctgc aaccagtgct tctggcagca gcccttccag gccagattca ctcagttgtt
    25081 gccctacctg caaaccaatg ccgtagcttg cttgcgcttc gatggtgtca aaagttgggg
    25141 tggtcatcgt atttcctttc ttagggtata aaagtagcgg gcagcatatc agccacactt
    25201 ctttgggtaa aactttgtcg caggaatgat gacaaatccc ggggaaaaag aaacaatagc
    25261 aatatcccgt atgaatataa taaaggcggg tttaccacgg acatcatagc gttagcttat
    25321 tcataggcta tgattgagga acaagacgtg gagcaggagg aaaccatgcc cgggcgcttt
    25381 gaactaaaac caaccctgga gaaagtctgg cacgcgccgg acaattttcg cttcatggac
    25441 ccgctgccgc cgatgcatcg tcgggggatc attattgccg ccattgtatt ggtggtcgga
    25501 tttctgcttc catctgatga tacgcccaac gcgtcggtgg tgacgcgtga agcgcagtta
    25561 gatattcagt cgcaatcaca accgccgaca gaagaacagc cgcaaactac ccaaacgcaa
    25621 ccattccagc cagatagcgg aattgataat caatggcgct cttaccgtgt cgaacccggt
    25681 aaaacgatgg cgcagctgtt ccgcgaccac ggactgcccg ctaccgatgt ctatgcaatg
    25741 gcgcaggtag aaggcgcggg taagccgctg agtaatttgc aaaatgggca gatggtgaaa
    25801 atccgccaga acgccagcgg cgtggtgacg ggcttaacca ttgatacggg gaataatcaa
    25861 caggtcctgt ttacccgcca gccggatggc agttttattc gtgcgcggta gatgaagggt
    25921 caaccaaaag ccagctaagg attgtctctt tgatgacctt acgtctggct tccagtgcct
    25981 ggggggaaac catactgata tgataaacta ggccaagcgt atgctggttg atcaaataat
    26041 atcccccaag cgcagcaata ttaatattca cctgcaatgg gtcgatacct ggcttaaaaa
    26101 tattgtattt cttcccttca tccaataagg attccattaa ctgcaagtga gcatggttaa
    26161 tttctaagag acgctgtgat tttgcataat gcacaccttt actttgattt tcactatgca
    26221 caatttttaa aaaccaagga ttagcaatat aataatccca tgtaaaatca ataattttct
    26281 caatggcttt ttgtggcgga agatctgaga aatcaagggc cttttctttt tgtcgaatat
    26341 cgttccaggt atattccagc acttcgataa ataaattttc tttattacca aagtggtgat
    26401 aaaccgtctg tttactacac cctgctgcct ttacaatatt atctactctg gccccttcat
    26461 aaccatattc ggcaaattca ttaaccgcac atagcagcaa tttttccttc aggctctgta
    26521 cgttgctggt atcaatatac ataacgcttc cccacgacct cttcgtggtg actatcataa
    26581 cctgatttga tttcagcttc agaaaaaagg gcaaaaactt gtactttcat caggttatca
    26641 aatgtcctgt gatcgccatc gcatatcaac caactagatg gtttatcttt ttatcctcca
    26701 gtcgcaagct aatcatcacc aaggaaacaa aattcaaaaa agagagctat tgtatgaaat
    26761 ccactcgtcc tgttgccgtg attaccggag ccactcgcgg tattggaaag ggctgtgcgc
    26821 aggagcttgc tcgtggcggg tttaacctgc tgattaacga tcgtcccgat gccgacagcg
    26881 tagaaaaact gcatatcaca caacaagaat gtttggcgga aggcgtggaa gtgatttgct
    26941 tccctgctga tgtcggtgat ctatctctgc atgaggagat gctcgacgcg gcgcaaaacc
    27001 agtgggggcg gctggactgt ctgctcaata acgcggggat ttcagtgaaa aaacgcggtg
    27061 acctcctcga cctcgaacca gacagcttcg atcaaaacat tgccattaac acccgcgcgc
    27121 cgttcttcct tgctcaggca ttcagtaagc gcttactggc acagccgaaa ccggaagcgg
    27181 agctgccgca ccgctcgatt attttcgtca gttccatcaa cgccatcatg ctggcgatga
    27241 atcgcggcga atacaccatt gccaaaaccg ccgtttctgc cgctgctcgt ctgtttgctg
    27301 ctcgcctttg taatgaacaa attggcgttt atgaagtgcg ccctggtctc atcaaaactg
    27361 atatgactat tccggcaacg gcttattacg acgaactgat tgccaaaggt ctggtgccgt
    27421 ggggacgttg gggttatccg gctgatattg cttccaccgt ccgcgcgatg gcggaaggta
    27481 aactgattta cacctgtggt caggcggtcg caatcgacgg cggcttaagt atgccgcgct
    27541 tctgaggctg aatcatgaga gatctgttac aacgcccgga tttattcagt atcaataccg
    27601 cgacactggg ctataaaacg ccgctacccg ccattattga tgcctgcgcc gcacgcggta
    27661 ttggcgcaat tgccccctgg cgcagggagt tgcagggtga agatctccag caaatcaccc
    27721 gccagcttgc cgccagtaac atgagcgttt ccggtttgtg ccgtagcact tactacaccg
    27781 cgccgacact ggcggaacgc aaactggcga ttgatgataa ccgccgggcg ctggacgacg
    27841 cagcggtgct taacgccgcc tgttatatgc aggtggttgg cggtctgcca acgggcacta
    27901 aagatctata tgaagcgcga gaacaagtga aacaagggat tcgccagttg ctgccacatt
    27961 cgaaagatgt cggcgtgcca attgcgctgg agccactgca cccgatgaca gctgccgatc
    28021 gctcttgcct gtgcacgtta cgtcaggcgc tggactggtg tgatgaactc gatcccgatg
    28081 gtgagtttgg cctcggcgtg gcggtggatg tttatcacgt ctggtgggat ccggatctcg
    28141 ccagtcagat cctgcgtgcc ggaaaacgca
//
LOCUS       DAJTQQ010000050        28108 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000050 DAJTQQ010000000
VERSION     DAJTQQ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28108
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.050"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(55..1347)
                     /gene="spaL"
                     /locus_tag="OO309_002310"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             complement(55..1347)
                     /gene="spaL"
                     /locus_tag="OO309_002310"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR5847429.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            complement(1351..1752)
                     /gene="spa15"
                     /locus_tag="OO309_002311"
                     /gene_synonym="spaK"
     CDS             complement(1351..1752)
                     /gene="spa15"
                     /locus_tag="OO309_002311"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR5847430.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            complement(1765..3825)
                     /gene="mxiA"
                     /locus_tag="OO309_002312"
                     /gene_synonym="virH"
     CDS             complement(1765..3825)
                     /gene="mxiA"
                     /locus_tag="OO309_002312"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR5847431.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            complement(3838..4905)
                     /gene="mxiC"
                     /locus_tag="OO309_002313"
     CDS             complement(3838..4905)
                     /gene="mxiC"
                     /locus_tag="OO309_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR5847432.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            complement(4924..6624)
                     /gene="mxiD"
                     /locus_tag="OO309_002314"
     CDS             complement(4924..6624)
                     /gene="mxiD"
                     /locus_tag="OO309_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR5847433.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            complement(6611..7243)
                     /gene="mxiE"
                     /locus_tag="OO309_002315"
     CDS             complement(6611..7243)
                     /gene="mxiE"
                     /locus_tag="OO309_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR5847434.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            complement(7373..7801)
                     /gene="mxiM"
                     /locus_tag="OO309_002316"
     CDS             complement(7373..7801)
                     /gene="mxiM"
                     /locus_tag="OO309_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR5847435.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            complement(7785..8192)
                     /gene="mxiM"
                     /locus_tag="OO309_002317"
     CDS             complement(7785..8192)
                     /gene="mxiM"
                     /locus_tag="OO309_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR5847436.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            complement(8185..8880)
                     /gene="mxiN"
                     /locus_tag="OO309_002318"
     CDS             complement(8185..8880)
                     /gene="mxiN"
                     /locus_tag="OO309_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR5847437.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            complement(8822..9349)
                     /locus_tag="OO309_002319"
     CDS             complement(8822..9349)
                     /locus_tag="OO309_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR5847438.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            complement(9346..10071)
                     /gene="mxiJ"
                     /locus_tag="OO309_002320"
     CDS             complement(9346..10071)
                     /gene="mxiJ"
                     /locus_tag="OO309_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR5847439.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            complement(10077..10370)
                     /gene="mxiI"
                     /locus_tag="OO309_002321"
     CDS             complement(10077..10370)
                     /gene="mxiI"
                     /locus_tag="OO309_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR5847440.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            complement(10383..10634)
                     /gene="mxiH"
                     /locus_tag="OO309_002322"
     CDS             complement(10383..10634)
                     /gene="mxiH"
                     /locus_tag="OO309_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCR5847441.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            complement(10642..11757)
                     /gene="mxiG"
                     /locus_tag="OO309_002323"
     CDS             complement(10642..11757)
                     /gene="mxiG"
                     /locus_tag="OO309_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR5847442.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            complement(11766..12224)
                     /gene="ipgF"
                     /locus_tag="OO309_002324"
     CDS             complement(11766..12224)
                     /gene="ipgF"
                     /locus_tag="OO309_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR5847443.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            complement(12224..12586)
                     /gene="ipgE"
                     /locus_tag="OO309_002325"
     CDS             complement(12224..12586)
                     /gene="ipgE"
                     /locus_tag="OO309_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR5847444.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            complement(12596..14212)
                     /locus_tag="OO309_002326"
     CDS             complement(12596..14212)
                     /locus_tag="OO309_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR5847445.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            14526..16010
                     /gene="icsB"
                     /locus_tag="OO309_002327"
     CDS             14526..16010
                     /gene="icsB"
                     /locus_tag="OO309_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR5847446.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            16023..16412
                     /gene="ipgA"
                     /locus_tag="OO309_002328"
     CDS             16023..16412
                     /gene="ipgA"
                     /locus_tag="OO309_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR5847447.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            16428..17054
                     /gene="ipgB1"
                     /locus_tag="OO309_002329"
     CDS             16428..17054
                     /gene="ipgB1"
                     /locus_tag="OO309_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR5847448.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            17111..17578
                     /gene="ipgC"
                     /locus_tag="OO309_002330"
     CDS             17111..17578
                     /gene="ipgC"
                     /locus_tag="OO309_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR5847449.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            17584..19326
                     /gene="ipaB"
                     /locus_tag="OO309_002331"
     CDS             17584..19326
                     /gene="ipaB"
                     /locus_tag="OO309_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR5847450.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            19346..20437
                     /gene="ipaC"
                     /locus_tag="OO309_002332"
     CDS             19346..20437
                     /gene="ipaC"
                     /locus_tag="OO309_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR5847451.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            20488..21486
                     /gene="ipaD"
                     /locus_tag="OO309_002333"
     CDS             20488..21486
                     /gene="ipaD"
                     /locus_tag="OO309_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR5847452.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            21495..23396
                     /gene="ipaA"
                     /locus_tag="OO309_002334"
     CDS             21495..23396
                     /gene="ipaA"
                     /locus_tag="OO309_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR5847453.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            23400..23636
                     /locus_tag="OO309_002335"
     CDS             23400..23636
                     /locus_tag="OO309_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5847454.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            23853..24782
                     /gene="virB"
                     /locus_tag="OO309_002336"
     CDS             23853..24782
                     /gene="virB"
                     /locus_tag="OO309_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR5847455.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(25729..26508)
                     /gene="ipaJ"
                     /locus_tag="OO309_002337"
     CDS             complement(25729..26508)
                     /gene="ipaJ"
                     /locus_tag="OO309_002337"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR5847456.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(27108..27245)
                     /locus_tag="OO309_002338"
     CDS             complement(27108..27245)
                     /locus_tag="OO309_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5847457.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            27861..>28108
                     /locus_tag="OO309_002339"
     CDS             27861..>28108
                     /locus_tag="OO309_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5847458.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
BASE COUNT         9538 a         5744 c         4121 g         8705 t
ORIGIN      
        1 tgtgcttcaa ttaatttaac agaccgttga tatttatctt tgatttttaa aaccttatct
       61 aattgtttca ccaataagct ccattgtctg ctcataagta aaaccgagtc gataatcttg
      121 cttcagaaaa ctctcaacaa cagatatttt gttgtagatt ttatcctgag aggcattctc
      181 ccccggtttg tattcaccaa agtcaataat tgtccttagc tcttctattt cagaaagtag
      241 ctcccgaaat gcagcggcca taatacgatg tttttcatca acaacctgtg taaataccct
      301 gcttatactt tttaaggaat caattgcagg aaattgtcct ttttgggcta gatttctgct
      361 caaatatata tgtccatcta aaatggatct tacctcttca gctaatggat cagcaaaatc
      421 atcatcctcc aaaagaacag tgtaaaatgc agtaatagag ccacctgcct ttaactttcc
      481 tggcctttca agaagtctgg gtaagctatc aaaaaccgaa accggatagc ctcttctggc
      541 aggtgattct ccagcggcta aggccacatc acgtaatgct ctggcatacc ttgttaatga
      601 atcaataaaa agcgctactt tatgtccttc agtcctaaaa aattcggcta tagcagtggc
      661 tatatatgca gcattacaac gatcaaccga agagtaatcc gaagttgcat atactaaaac
      721 acacctgctt tttttctcag agtttttcaa ataatcaacc gtttcagtaa cctctcgacc
      781 tcgctcacca attaacccaa taacatatat atcagcacca ctatgttcaa tgagcatatt
      841 catgagaaaa gttttgccac aaccagctga cgcaaaaatc cccattcgct gtccttcacc
      901 acacgtgagt aaagaatcaa taaccttaat acctgttaaa aaaggcttct caattgcagc
      961 cctttcacta tatagcggag gagcattatc tacaggtcga taaagaattt cactgttatc
     1021 tgtaacagca aacttatcag taacctcccc taaaggattg actactgctc ccaataatcc
     1081 acggccgact tgcgtatgaa gaaactgggc agtgggctta atcaacgttt gccgtgaaag
     1141 tccacgagaa tttccaatca agcttaatat tgttttttca tcatgaaatc ctacaacctg
     1201 agctcttgca acaatttcat tactttcaat tccagcctga atgttacaaa tctcaccaat
     1261 cgaaacatcg ctaagacttg tttccaagat tggccccgag attctattag gaaaagataa
     1321 ttgagtgagc aattttgtat agctcattga ttataagacc ccatttaaga tttccatcct
     1381 ctgataaaac tcatgcagaa tctctgcaaa aacaatccca tcgtgaacgt agtcatcttt
     1441 aataacaact cttaactgta agtactcatc agaacgatga agctctacca gctcattaat
     1501 gctataacta aagttcatta acattaaatt taatatgtta taagcagatg actgaagttt
     1561 tacatcactt ggtgcatcaa aattagccca taacataacc tgctcattga caagagctat
     1621 attaatagca ggcatactat ccaaagaaat ggtaatcgct gaatggctgt ctaaatctgt
     1681 aataattgat ggaggacaac caatcgtgaa aagactatct ctaactaatt gaactaaatt
     1741 aatgttactc atatttaaac ctcactaaat agtctttaat acattaatgg tatatgcttc
     1801 atcaatctca gcatacgata taacgagtat actcttaaat ctgttatcta taagtctttt
     1861 aacaaaccta cgtatatcta ctgataccaa aagaacgaaa tttttttttg catttctcaa
     1921 ttctctcaaa gcatgtgcta aagtttccat tacctcatcc gaaacctcta tatccatatt
     1981 caagaaagag ccaccagagg tttgccttat cccttttctt attgcatcct caatatatcc
     2041 ggaaagcatc acaactttaa tctcaccaga aacagctatt ttactacaaa tatacctaga
     2101 aagtgatgca cggacatgtt caactaatgt tatgacatct ttttctctgg gagcccaaag
     2161 cgccaaagac tccataataa gttttaaatt gcgaacagaa atattttctc caagcaatct
     2221 ttgtaatacc tcagaaattc tctgtatagt cacatgtcgg aagacttcct ttaatagatc
     2281 aggataccga ttttcaaact gatctaacat attttttgtt tcttgtatac caaaaatctc
     2341 atttatgttg tttaataaag cttgtgacaa ctggtgataa aattcatctt gagcactttt
     2401 aattttagca tcaatattag ttagattttc tgtatatgaa acgtctaccc aagaaataac
     2461 acgttcatta tatgaagttg aaacaacagg aatacctaga gcatctatat ctccattttc
     2521 atctgtaata cacactttgt caaaatatat attaaaacta tcggcacgca cctcatttat
     2581 taacaaaaca atatcatcga ccttaagctc attgcttgtt ctatataaaa tagttggaag
     2641 cctgacacca taatcaataa agaactgact tcttatcctt tcaataagac cttccatatc
     2701 atttgcattt atcttatttt cggcaaataa taaaattaaa ggaacggttt ctgaactaat
     2761 agcatccaga ttttctatca ttgccaaaga tgagtcatgg ctattatcaa tatcaaatgt
     2821 accagtatag ccactagaat cagactcaga cagacttttt tctttttcta cgaccttttt
     2881 ataatagaat aaagccgtca aagtaactgc tatcaggaaa aaaacaaaaa acggaaaacc
     2941 tggcaacatc cctatcgcca aggcaagagc tgatgtaaca atgagaacaa aaggattccc
     3001 aaatatctgg gacattatgt tacgtcccat attatcgcta tcaccattaa ctcttgtaac
     3061 gataaatcct gcacttatag aaattaacag agcaggaatc tgagagacca atccgtcacc
     3121 aatagttagt atggtataag tagataatgc accagatagg gacattccat gttgactcat
     3181 accaacagaa atacctccta ttaaattgac aaaaataata atgatgccag caattgcatc
     3241 acctttaatg aacttcattg ctccatcaaa tgaaccatac aattgactct cacgttccag
     3301 aatactgcgt ctttctttag ctcctgcggc atcaataatt ccagccttta aatccgcatc
     3361 aatgctcatt tgtttccccg gcataccgtc aagagaaaag cgagcagcta cctccgcaac
     3421 cctttcagat ccttttgtta taacaatgaa ctgcacaaca gttactattg aaaaaataac
     3481 aaaacctaca gccagactat caccaatgac aaactggcca aatgtagtaa taattttacc
     3541 cgcatccgca tcaactaaaa ttagtcgact agtacttatt gataaagcga gacgaaataa
     3601 tgtcgttatc aacaaaaccg aagggaatgt agaaaaactc aatattcttt caatataaaa
     3661 tgatcccata aaaacgagaa tagcaagtac aatattaagc cctattaaaa aatcaacaag
     3721 gtatgtaggt aatggtatga tcaacatagc aatgatcata accataagca ctaatataat
     3781 taattcaggc ttagtactta cttgcttaag aaaagactgg atcactttta tctcctgtta
     3841 tctagaaagc tctttcttgt atgcacttgt gacaagctct cgtagaatat tgatcgcaat
     3901 ttctctttca ctttcacttt taaatatatc atgaggactt tcattaacag aagatagata
     3961 tgtttgatac actacagaat cctcatgact ggctcgtgat acttctataa cagaagtaag
     4021 aatttgctca ctttcacttg gatattgaaa aatatataat aaagttagca atagctgctc
     4081 tgcacttaaa ctgccatctt ttagcaagct tgaggattcc agtttcttca taaaaattac
     4141 gtctgaagtt ctaatcatct taattgcaat taatttagat aacacaaaac caaactcata
     4201 agcattacag ctcggtttct cagaatccat gtctacaatc agtgactgct ctacaaaatt
     4261 cacaattgta tgtctgtgat taaaaccaaa attaccaagc cattctatat actgatctgt
     4321 tgttgatatg ttccccatga taaaaccacg ataacatgca cgcaaaagct gtggttcaag
     4381 tttcattttg ttgccaaata atttagcctg tatagctgaa tttattccag cattaacact
     4441 tttttcacta cctgatatta tctcattaat aattttagtt aataattcga cttgttcagc
     4501 agtaagatct ttttcattca aaagttccct taatgctaag acttgatcac ttggatcttt
     4561 aaaatatctc tttagtctgt ctataaaatc ccgatctaga ggaaggttat cttttaacgt
     4621 tctctttaaa tcaaaaatct tgtgattaat ttcatcttct tccccatcta aaatacgttc
     4681 ctgactatca ctatttgttc ctttcagttt ctcaaggtct cttctgttta taaatgacga
     4741 aagagcggaa gccatctcat cagatgagtc aatatacttg ctattagcca agccagaatc
     4801 aagctctgca tcagcagtct catctccatc atctgaattt gtcattgcat tcaacttatc
     4861 aatgaatgca gagctaaaaa ctcctgtatt tttaacatca agcatacatc acctacttta
     4921 tagttagtaa tttaagtatg aaaccaatga tttttcgtct tccagcaaag tagtttcaga
     4981 tgggattatt tgtgtagtct tttgtatttc tctttcagat atgaggcttt tatattctgc
     5041 agtattataa taacttgact cctttatctc acggggttga atcagaaaaa cccttacaat
     5101 attactgata ttacttgttt tatacttgaa cacattaccg attacaggaa tagaggagag
     5161 aaaaggaata gatattattt cgttagagtt cgtttcatgc gtataaccac ctatcaacaa
     5221 acttttgcct tgtggaactc tcgctattgt actgattttg gtccttccaa cttctggaag
     5281 gacagatgta ttttcattat tataattgta atttgactga ctatttcctg ttccatcctc
     5341 tatcgttaaa gacatctcaa tttgtccccg agatgaaaag cgaggtataa catttatcaa
     5401 tgttccatac gtaacatgct ccaacgaaga attgcgctca ccaactaatg acacataaaa
     5461 agtcctgtta ttatcaaata tagccggaat attctcttgt gtcaaaatca ccggccttga
     5521 aactacattt gctttctttt tctgatttaa tgccataact gaagcaataa acttattgcc
     5581 gtccaaagtg cttatcgatg ctgatgagct catattaaat gacgcaccaa acgaatcacc
     5641 aaatgaagcc gttccttgcc aattaacacc aaggttattt aattcagatt tatcaatatc
     5701 gattatccat aatgataatt ctatatgtcg tttggctata tctaactgag tgataatatc
     5761 gcgaataatc tgtatttgct gatcattacc tttgactaat atagaattgg tttctggata
     5821 tgcaataagt gatacatcct ctaatataga actattggta actgaactaa atgaaaagtc
     5881 attactatcc tcagatactt tctgtgtaat attaaagggt ggcatcggat cattttgggc
     5941 ttgtctgtta ctcagagcct taccattatt taataaacgt tctacaacag tagccacccc
     6001 tgggatgaca atatcctccc ctctcatatt ataagttctg tcactaacaa atgtattttt
     6061 taattttata accccaaaat taactttatc tgtcccaata gaagaaacct gcttatcaag
     6121 caaagttgct gtattagcta ccagttcaac caatgccgga ggaccagata tatagaatgt
     6181 tttatcacta atgtttcctc ttataggata tctgtgatca taaaggttag catccttcag
     6241 atactgaata agataattaa gtgatatatt ctccagcaaa ataactttgc ttattaattc
     6301 tccagagtca tatatgtata atgcatttcc atctttatac catatgagtc ccacaagtaa
     6361 tgtcagtttt tcaagcattt cttctggatt agataaatca aactccccag atattctctt
     6421 ttttgcagct tgtttgctaa caacaatagg ataatttaat aacgcagaaa aacgttcaaa
     6481 aaaagatcct accgtatcac tttgagcaac atatttcgct atgtcatttt gttctaataa
     6541 atgagagtca atattattag cattaactat caatggtaac aatacaatca agagagtcaa
     6601 agatttaata ttaaattttt tcatttattt ttttcactaa aaaagtaata ttcgataatt
     6661 ctcttgcaga aaagcctagt cgtgttttta tctcatttga aaaatgggat gttgatgcat
     6721 acccattatt catggcagct gaagtgattg tttgattgtg caaaaataca tctaataaac
     6781 catttactaa ccgccaagtg ttcagttgtt ctttgacttt tgcccctaaa gcttttctac
     6841 ataaagatct aaaataagcc tccgacacac cataatgctc tgtcagactc tttattctta
     6901 cctccttttc tttctcaata gttctcagaa gataaagaac caaatgatat gactcagtca
     6961 ttcgtatcat agccagtgca ttacttacat tctggtcaga tgaaaagatg aatcttaaca
     7021 ttaacttatc aagcatcgat tcggtattac aactatcccg aagatcattc tcatgataga
     7081 aatgaataca tttttcagca tggctatgct ctactagagc accactgaaa tagtcccaaa
     7141 aagcaagaaa ttttgcacaa ctattaatgt taaagcgata atgatagttc acattatcac
     7201 tgaattttat attttgattt cggacaaaaa aaaacccttc catgctggag ccagctcaat
     7261 tatgttacgt tctttatctt ttacaagctc aacgttaact ggttcatgtc cactagagta
     7321 aatgtaaaac atattacttg tatttagacc tttatattta ctcacaatac aattatatcc
     7381 ctcgaattaa ttttaattta acttccccat ccttctctat aaggatggta tcatttatct
     7441 tattaccttt taaaacaacc ccgtctttca gcatagaaaa tagtttatct atagctatct
     7501 tttgtgcatc catgaagcac ccatctactt ccttaacatt aaagttgccc atgaatttac
     7561 ctgcattaaa actataaacc gccttaccac taatacattt agagtaaaca tttatacttt
     7621 tattggttgt attaaacgac cataataaat catttggaat agaaaaataa tccttactta
     7681 caggaacaat atgccattct ttttcagaat tagatgatga ctttaaagca cacccactca
     7741 gtgttaatag caatatactt aaaataaata ttttcaactt attactacca tgtcgaatca
     7801 tctgcctctc tccctgcttc aaagacacta attaatgaat cgaaggttgt agtcttaata
     7861 tagaaatcaa tattgttata tatggacaat gatgcataaa atccctcatg caaatcgaca
     7921 tattgatttg catctatgag tttattaaga agtgaacttt tttgtgcagc tgtaagataa
     7981 ggaaaacgtg aattgaataa ttgttcaatc ttattgtgca gttcatcatt atctatggaa
     8041 tttaattcac tctgtgagaa atattgcatt atttcataga ttatatcctc atcaaatgat
     8101 tgcgaagatg ataatctctc atttaaatta acaacttctt cactatttaa tctttgctgc
     8161 agagcattgc ttgcatttat ttgattaatc attaacagga ttctctttta acaagtcttc
     8221 tgcaatttgt ctcagtttct tatgtgctaa ctctttgaaa tattttttat cattttttgt
     8281 taatttttcc gcaagttcta ttttaacacc tgatattaca tcctgaggac taaactccac
     8341 tacttgatta ccactacaga atatataatt attcccttca gaatatttta aaataatctt
     8401 gacatctgag cgatatttat ggagatcaag ttctaacttt ttcctcagtg ctaaattaca
     8461 cttgggcaaa accactttta attctgttac tgtattacgc aacgaactaa gccactgctc
     8521 caataaagtg ctaatgatcc tctcatcatg taaattttct gaaagcaaac tagttatata
     8581 gtcctcaata tttcttctat tttcattttg ctttaacttc cattcacaaa tatagtcaat
     8641 aatatgctca aaagcagtct gtataccaat ttgataacca tcacatacag catctatacg
     8701 cagtgattca gcttttttcg tagcctcctt tataacacaa attgctttct tcttactctc
     8761 agtgtctcta tcttttatgg ttttacataa ttccttttca attctcttta taacaacacc
     8821 atcataggca tgatgtctgg ataccggtaa agtgcctttt tgcatattgc ataccttcat
     8881 ttaatcggga caaatttctt tctacaggcg tataatttcc ctccaaagca attggattaa
     8941 ataataagtt tattctttca tatataccaa cagatatatt ttccttccac gcaataagat
     9001 gccaaaaacc acttcgaata gcggcctccc cttgcttact gattttgccc ccataataac
     9061 taataaccat ctcactatga ttaattaacc gagactcttg actaccgata aaataagcaa
     9121 cagctggaag taaattccag ttggcaacaa acatagcggt taccccgctt aaaggttcaa
     9181 tatcgcaatt tagatcatat ttattaatta tgagattatt aatctcactt ctgattacgc
     9241 cattttctaa cagttcagaa ggcaaattca accgatttct atttatatag aacgctggat
     9301 caaaaattat tgaaagatat tttttataaa ttccatccat tcttatcata ttttgtttct
     9361 cttgaaccac cctgttttga atgcccatac caaaagaatg acaactagta cagccatccc
     9421 gagaaataaa tatattactt catttgttaa aaattccgat ttaacttcct taacaggttg
     9481 tacatttgta taaacatatt cttctttcgg agttaatatg acagatatat tttcatactt
     9541 aacatcagaa aaggtgtttt tcaaaaatcg cttaatatta ctaactaata gttcagactc
     9601 tttcggtgag tcatatatag cgattactga tatatgcatc ggttttgaag atatattttt
     9661 ttcttcaaga tcatagctaa catgtatttt tgccgaaata acaccaccaa tagaaactaa
     9721 agactgttcc agccgttgct caatagcact atataaacgg gccttttcag ctcttggaga
     9781 agacactaat gaatctgtag gaaacatttg tgagatatca actctctccg gatttggcaa
     9841 atcgtacatg cgcatcaaat caactgccga tgcaaatgtc cccttttcga cttgtaccga
     9901 gatcccctgt ttacctccat caacttttct agcagtaata ttatggcgtt ctagcacaga
     9961 tattatttca tttgcctgtc tttgagataa attagaaatt aactcttcac gttgctcaca
    10021 tccaatcaac atcagcaaca agaataaaat aaaaccttta tacctaatca tataaattaa
    10081 gactttaata aagtttcaac agccgaaact gtttttcttg ctaacgtgcc agctaatgat
    10141 actcctattg aataatttga gagcgtcgtc tgtaacttgg ccaaagattc tgggtttaat
    10201 gattctggat tgcttaccat ctccattatt tgtgatactt gaatatctgt atccatctta
    10261 atatcactat atttagccga cacgacgtct tccagacttg atatctcttg agattgaaaa
    10321 tcactggctt tgataatatc aacctgattg actggataaa tgtaattcat gaatgctccc
    10381 tattatctga agttttgaat aattgcagca tcaacatcct taatcacttt cactgtattg
    10441 gattgcgcgt tcctatataa tgtatattca gataatttac tttggtattc agccagcaac
    10501 tgtggattcg aaggattttt agctaattta tctagtgcca atgttagttc accttgtaat
    10561 gtttgagttc catcatcaaa agtttcagat aatgaactca atgtccaatc atcattcggt
    10621 actgtaacac tcattttatc ctcacttatt tttatctaaa aaaaaccagt gtttatcatt
    10681 caacattaca taactgtctt tgctgttaag atatgattta cctttaaaat catcatctat
    10741 caaaagtaca gaaaactcaa tatactgatc tccatatata tttttatgtt cagaaatcag
    10801 tctattaatg agctcaagct tttcatctgt cagttcttct ctgacagaat atgtaacttt
    10861 attattttca caaatctctt tatactggac atttaccttt gtgaaaatac ctttagcatc
    10921 cccccttgca ttatgatcgc taagatagtt gaatttaata tttttgatat acggaaattc
    10981 aacaatcaga gcatttttca atttatcaag ttcggtatct tttgaactat ttctttcttt
    11041 acttaaaaac accagtaatt ccgctttatc actcactaaa ttcaacacat acaaattgat
    11101 aaatgggaat cgttgattga tatattgttg aattctattt atttctttgt ttgacaccag
    11161 aatatatctg catttggtaa atccggtttt atttaaggct acagacgccc ataccgcatc
    11221 attttgtgtt tcggcaagaa tataattgca ctgtccagtg ttgactattc catatctctt
    11281 atcatctata atttcagcaa tttcttttac ttcatctttc tttaaagaga gggaaaaaat
    11341 tataattaac acaacaataa cagcaaagaa aaaaaacacc gagtggttct tatacattcc
    11401 gtttagtata tggcctctcg atttatcctc cctcatattt ttaaggtgaa aagaaatccc
    11461 gtcatattca aatgaattaa attgtgaatt gatatcaatt tgctcacctt tttcatcact
    11521 aaattgacag atattatctt tgcttatggt caaataaatt ataccatccg atttactatc
    11581 tgttacagga ataatatttt ctgggaaatt gtcgtttcca aactcaagtg tttctatcgt
    11641 ccgtccgaga acaattaagt tattcccata atataacgga aactcatccc cgacaccgtt
    11701 tgtgagtttc actaataacc tgaatggtgc aagatttgag ttctttgcct cagacataaa
    11761 tcctcttata tccttcgatt attctgcttg ctctctgctg ctatccgcaa atagcgtcta
    11821 tagatatcct ctgcgtactt taatctttct tttttctttt tcggagacgt tcctgcatta
    11881 taggctccaa cagcctccca ccctcttcca tacatcatca taaactcatt taataacttt
    11941 gctgcataat gtacagaaag acatggatga ctaattagca tttcctcaga atatcccatt
    12001 tctctaagtc ttttagaatg aaagtcattt atttgcatta taccataatc tttacttcca
    12061 ttgttattaa cattgactgc agatttatta aatccggatt ctttttccgc aatcgctttt
    12121 aacaaacttg atggaatatt gtacctttca ccagccttat cccaacaatc agctctccct
    12181 aaatatggga taaaacacag caaaataaat acgaaacggg acattaatac cccttcattc
    12241 ttcgcgcaaa ttcatccttc agccaacgca cacgcgaaat ataatattct gtattaacat
    12301 atacatcctc gaattcattt atcccggtaa gatctaatcg cgcaattaga tttccgcctt
    12361 catcatcagt agctaagcat atcttttctg agtaatttag gctcaacgca tatataagat
    12421 catttatatt ttcaggcaat gcacaaaaag ggcataataa atttattgaa tctccttctt
    12481 tttcaatata tattagcaca tcatcatcaa gcatgattgc ttgatcatcg atatcaatta
    12541 aagggatacc caaggctcgg caaataacat ctgctaaatc ttccatatat tcctcttata
    12601 caaatgacga ataccctttc accatattcc atatttttgg gtcccctatt ctttcagaat
    12661 aagatagctc taacgaagat agcggcaatt ttttcatgac tttatttccg ggcacaccag
    12721 tattcatctc ttggatttcc atattaccac tattcattag aatagtagaa aataatcttt
    12781 tctcttctga ggataattta ctatttaatt gagaaaactg acctgtttca tgttttctta
    12841 ttatttctct tttaatttca gcatcctgca tacctgttct gtccttccca ctcttacaat
    12901 tccagcaagg tacagcacct atagtataag ccaatagagt cattctttgt gacaatttat
    12961 acggctctcc attatcattt ttttgtaact ttttggttac aatctctttt atctggttag
    13021 ccagatatat cacatcattc ttacatggtg gatttttttc tattgcttct gcagcccaac
    13081 ctccaatcac accatttttg aggaaattat cccccaacaa agaacatatt gattcatcat
    13141 taagcttgtc aacattcctc cagcctaagc ccatttttag cgctaattca tttacaccaa
    13201 agttaaatgt caccactttt agattaacgc ttacttcttt cagaagacca tcactattcc
    13261 gaattaataa tttcgttggc ttcccccttt tagagtttaa cccttttaat gcgttcacct
    13321 gatcttttaa catactctcc tctcccccgg ttaaactggt cggcgtcaga agagaagtcg
    13381 aaacaatctt taaatctact gttttaccag acaaagcctg tgataataac tctggtctgc
    13441 tatataatgc tgcgcttact aactcctcag ctttattacg agcagcaaca gctctttcag
    13501 atgagttttt tttcaatcca taggctgata taactccatg tctgataccc gaaaaaattt
    13561 ccttcccctc atcatccacc acttttgaaa gccacatatt cgcgatatga tcactctctc
    13621 tcgtagaagc acaacaaatt ccttttccat tatactcttt gacaaatata tttttattcc
    13681 ctatcttcat atgagaggca ggagttaatt gaaaaccata atttttccca tgatgactaa
    13741 tattcttgtc gaccggtccc caattcttat tatttaaata atcagtaaat atttgtttta
    13801 ttttcgtttg caattccttt ttatttttaa tgcctagctg tgctcttatc tcttcactta
    13861 ttactttatt aagctcatta atatgtcttt tattaacttc accaagttta taggtatcta
    13921 atcgttccat catggcttta atttttgacg tatcaccacc tgccaacttt attgcctcgc
    13981 aaaaaacttc agctctgtca cgcgcaaaca aaaggctttt tcctgttaag cttgtttggt
    14041 tctgtaaata taatcgatta agcgcttcga tccccttatt tctttctcca tttttaactc
    14101 tttgatatga aatcacgctt acacctttgt gctttccggt ttcttctgcg cctttatagg
    14161 aatctccgct ttgaaatgaa acctgatgca atcccaaatt agttatgtgc atttaattat
    14221 cctcagtcat aaatggggca ttgattgcag ttttgtacct cgttttacaa caaaaaagat
    14281 acttattcaa cagggttctc tctttttcta taacactccg agcctgatac atcaggcact
    14341 ttatcttgtg ggatttcatg atgaaacgag cactacatat gctcacgagg tacatattat
    14401 gtacagcaaa agaaaatttg attatgttaa caggctcaag tttctaaatt tattaattga
    14461 atatattata taccaaatac attttacatc cccacaatca ccaagtaatg gagagttaat
    14521 aaagtatgat cctcaaaatt agcaatttca ttgacgcaag caatacaaaa gggcctatac
    14581 gcgttgaaga tacagagcat ggacctatat tgatagcaca gaaattcaac ctaaaggatc
    14641 tctttttcag aacattaagc actattaatg caaagataaa ttcacagata ttaaatgaac
    14701 aactaaaaaa ttatcgacta gagaatcaaa aatccttatt gcttttcctc aatacattag
    14761 ctagcgaaaa atctgcagaa agtgcgtttg ccgcctatga agcagctaaa aactcaattc
    14821 aacactcttt cacaggtaga gacatcaaat taatgttgaa taccgcagag cgttttcatg
    14881 gcatcggtac agccaaaaat cttgaaagac atttagtttt tcgttgctgg ggaaatagag
    14941 gcataaccca tttagggcat actagtatca gtataaaaaa caatttacta caggaaccaa
    15001 ctcataccta tctcagctgg tatcccggcg ggaatgttac aaaagataca gaaataaact
    15061 acctttttga aaaacgctca ggttacagtg tcgataccta taagcaagac aaactaaata
    15121 tgatttcaga ccaaacagcc gaaagacttg atgcaggcca agaggttaga aacttattaa
    15181 attcaaaaca agatcaaaat aacaacaaaa aaatattttt tcccagagct aatcaaaaaa
    15241 aagaccccta tggttattgg ggggtcagtg ctgataaagt atacattcca ttatcaggtg
    15301 ataataaaac aaaggatggt aaaatcagcc ataacctttt tggccttgat gaaacaaaca
    15361 tgtcaaaatt tatatgcaaa aagaaagcag atgctttcag acaattggca aattataaat
    15421 taatcagtaa atctgaaaac tgtgctggta tggcacttaa tgttcttaaa gcaggaaatt
    15481 ctgaaatata ctttccactc cctgacgtta aattggttgc tacacctaac gatgtatacg
    15541 cgtatgcaaa taaagttagg caacgcattg aaagtctcaa tcagtcttat aacgaaataa
    15601 tgaagtatat tgaatctgat tttgatcttt ccagattgac tcaattacga cgcagctatc
    15661 ttaaaagttt caataaaatt aaccttatcc acacaccaaa aacattcaag cctttatcaa
    15721 tttcacttta caaacatcct actgaaaatg tatcttcaga agactttgat gcggtcatca
    15781 acgcttgcca ctcatactta gtaaaatcag cacccagcaa tatgactcgt gtattaaacg
    15841 aattaaaaac cgaagcaaca gataaaaaag aagaaatcat cgaaaaatca ataaaaatta
    15901 ttgattatta caattctctt aaatcgccag atcttggaac caaactttac attcacgact
    15961 tattacagat caataagctt ttattgaata actctcattc taatatatag aaggccatag
    16021 aaatgtgtcg caaactatat gataaacttt atgaaataac aggtgcaaag cttgatttta
    16081 acgataaaaa tcaagccttt atattgctcg aagaacaaat acctgtttgt ataacagata
    16141 atgatgaata catctttttg accggacttc ttaatgaaca tgaacttttt acagaaaata
    16201 ttattaatcc agaacacata ctcatcctta actactcttt gagtagagat tatggttctt
    16261 ctatctgtct tctccctgat actcatcaat gtgttttgac caagaaacac tacaaaaaat
    16321 atctttcacc tgatgaactc attgaatctt tgtatgaatt tttattttgt attaaactaa
    16381 ccattgcaaa catcacttca gaagtgaact aacatatagg gggtatcatg caaattctaa
    16441 acaaaatact tccacaggtt gaattcgcga ttcctcgccc atcatttgat tcgttaagtc
    16501 gcaataagct tgtaaagaaa atattatctg tttttaactt aaaacaacgc tttcctcaga
    16561 agaattttgg atgcccggtg aatataaata aaatcaggga cagcgtcata gacaaaataa
    16621 aagatagtaa ttcggggaat caactatttt gttggatgag ccaagagcga accacttatg
    16681 tctcttcaat gataaacaga agtatagatg aaatggccat acacaatgga gttgttttga
    16741 cctcagataa caaaaaaaac atatttgcag ctattgaaaa aaaattcccg gatataaaac
    16801 ttgatgaaaa atcagcacaa acatctatca gtcatacagc actaaacgag attgcctcat
    16861 ctggcctcag agcaaagatt ttaaaacgct atagcagtga catggatttg tttaacacac
    16921 aaatgaagga tcttacaaat ctagtatcca gttccgtcta cgataaaata tttaatgaat
    16981 caacaaaagt tttacaaata gaaataagcg cagaggttct aaaggctgta taccgtcaaa
    17041 gcaatacaaa ttaaatcttt tatattaaat cttatactta ttaaagtaaa gcagacaaaa
    17101 aggagacctt atgtctttaa atatcaccga aaatgaaagc atctctactg cagtaattga
    17161 tgcaattaac tctggcgcta cactgaaaga tattaatgca attcctgatg atatgatgga
    17221 tgacatttat tcatatgctt atgactttta caacaaagga agaatagagg aagctgaagt
    17281 tttcttcagg tttttatgta tatacgactt ttacaatgta gactacatta tgggactcgc
    17341 agctatttat cagataaaag aacagttcca acaagcagca gacctttatg ctgtcgcttt
    17401 tgcattagga aaaaatgact atacaccagt attccatact ggacaatgcc agcttcggtt
    17461 gaaagccccc ttaaaagcta aagagtgctt cgaactcgta attcaacaca gcaatgatga
    17521 aaaattaaaa ataaaagcac aatcatactt ggacgcaatt caggatatca aggagtaatt
    17581 attatgcata atgtaagcac cacaaccact ggttttcctc ttgccaaaat attggcttcc
    17641 actgagcttg gagacaatac tatccaagct gcaaatgatg cagctaacaa attattttct
    17701 cttacaattg ctgatcttac tgctaaccaa aatattaata caactaatgc acactcaact
    17761 tcaaatatat taatccctga acttaaagca ccaaagtcat taaatgcaag ttcccaacta
    17821 acgcttttaa ttggaaacct tattcaaata ctcggtgaaa aatctttaac tgcattaaca
    17881 aataaaatta ctgcttggaa gtcccagcaa caggcaagac agcaaaaaaa cctagaattc
    17941 tccgataaaa ttaacactct tctatctgaa actgaaggac taaccagaga ctatgaaaaa
    18001 caaattaata aactaaaaaa cgcagattct aaaataaaag acctagaaaa taaaattaac
    18061 caaattcaaa caagattatc cgaactcgac ccagagtcac cagaaaagaa aaaattaagc
    18121 cgggaagaaa tacaactcac tatcaaaaaa gacgcagcag ttaaagacag gacattgatt
    18181 gagcagaaaa ccctgtcaat tcatagcaaa cttacagata aatcaatgca actcgaaaaa
    18241 gaaatagact ctttttctgc attttcaaac acagcatctg ctgaacagct atcaacccag
    18301 cagaaatcat taaccggact tgccagtgtt actcaattga tggcaacctt tattcaacta
    18361 gttggaaaaa ataatgaaga atctttaaaa aatgatctgg ctctattcca gtctctccaa
    18421 gaatcaagaa aaactgaaat ggagagaaaa tctgatgagt atgctgctga agtacgtaaa
    18481 gcagaagaac tcaacagagt aatgggttgt gttgggaaaa tacttggggc acttttaact
    18541 atcgttagtg ttgttgcagc agctttttct ggaggagcct ctctagcact ggcagctgtt
    18601 ggtttagctc ttatggttac ggatgctata gtacaagcag cgaccggcaa ttccttcatg
    18661 gaacaagccc tgaatccgat catgaaagca gtcattgaac ccttaatcaa actcctttca
    18721 gatgcattta caaaaatgct cgaaggcttg ggcgtcgact cgaaaaaagc caaaatgatt
    18781 ggctctattc tgggggcaat cgcaggcgct cttgtcctag ttgcagcagt cgttctcgta
    18841 gccactgttg gtaaacaggc agcagcaaaa cttgcagaaa atattggcaa aataataggt
    18901 aaaaccctca cagaccttat accaaagttt ctcaagaatt tttcttctca actggacgat
    18961 ttaatcacta atgctgttgc cagattaaat aaatttcttg gtgcagcggg tgatgaagta
    19021 atatccaaac aaattatttc cacccattta aaccaagcag ttttattagg agaaagtgtt
    19081 aactctgcca cacaagcggg aggaagtgtc gcttctgctg ttttccagaa cagcgcgtcg
    19141 acaaatctag cagacctgac attatcgaaa tatcaagttg aacaactgtc aaaatatatc
    19201 agtgaagcaa tagaaaaatt cggccaattg caggaagtaa ttgcagatct attagcctca
    19261 atgtccaact ctcaggctaa tagaactgat gttgcaaaag caattttgca acaaactact
    19321 gcttgataca aataaggaga atgttatgga aattcaaaac acaaaaccaa cccagatttt
    19381 atatacagat atatccacaa aacaaactca aagttcttcc gaaacacaaa aatcacaaaa
    19441 ttatcagcag attgcagcgc atattccact taatgtcggt aaaaatcccg tattaacaac
    19501 cacattaaat gatgatcaac ttttaaagtt atcagagcag gttcagcatg attcagaaat
    19561 cattgctcgc cttactgaca aaaagatgaa agatctttca gagatgagtc acacccttac
    19621 tccagagaac actctggata tttccagtct ttcttctaat gctgtttctt taattattag
    19681 tgtagccgtt ctactttctg ctctccgcac tgcagaaact aaattgggct ctcaattgtc
    19741 attgattgcg ttcgatgcta caaaatcagc tgcagagaac attgttcggc aaggcctggc
    19801 agccctatca tcaagcatta ctggagcagt cacacaagta ggtataacgg gtatcggtgc
    19861 caaaaaaacg cattcaggga ttagcgacca aaaaggagcc ttaagaaaga accttgccac
    19921 tgctcaatct cttgaaaaag agcttgcagg ttctaaatta gggttaaata aacaaataga
    19981 tacaaatatc acctcaccac aaactaactc tagcacaaaa tttttaggta aaaataaact
    20041 ggcgccagat aatatatccc tgtcaactga acataaaact tctcttagtt ctcccgatat
    20101 ttctttgcag gataaaattg acacccagag aagaacttac gagctcaata ccctttctgc
    20161 gcagcaaaaa caaaacattg gccgtgcaac aatggaaaca tcagccgttg ctggtaatat
    20221 atccacatca ggagggcgtt atgcatctgc tcttgaagaa gaagaacaac taatcagtca
    20281 ggccagcagt aaacaagcag aggaagcatc ccaagtatct aaagaagcat cccaagcgac
    20341 aaatcaatta atacaaaaat tattgaatat aattgacagc atcaaccaat caaagaattc
    20401 gacagccagt cagattgctg gtaacattcg agcttaatat atccaagagc cataataata
    20461 tatggctctt cctgtaagga aataaccatg aatataacaa ctctgactaa tagtatttcc
    20521 acctcatcat tcagtccaaa caataccaac ggttcatcaa ccgaaacagt taattctgat
    20581 ataaaaacaa cgaccagttc tcatcctgta agttccctta ctatgctcaa cgacaccctt
    20641 cataatatca gaacaacaaa tcaggcatta aagaaagagc tttcacaaaa aacgttgact
    20701 aaaacatcgc tagaagaaat agcattacat tcatctcaga ttagcatgga tgtaaataaa
    20761 tccgctcaac tattagatat tctttccagg cacgaatatc caattaataa agacgcaaga
    20821 gaattattac attcagctcc gaaagaagcc gagcttgatg gagatcaaat gatatctcat
    20881 agagaactgt gggctaaaat tgcaaactcc atcaatgata ttaatgaaca gtatctgaaa
    20941 gtatatgaac atgccgttag ttcatatact caaatgtatc aagattttag cgctgttctt
    21001 tccagtcttg ccggctggat ctctcccgga ggtaacgacg gaaactccgt gaaattacaa
    21061 gtcaactcgc ttaaaaaggc attggaagaa ctcaaggaaa aatataaaga taaaccgcta
    21121 tatccagcaa ataatactgt tagtcaggaa caagcaaata aatggcttac agaattaggt
    21181 ggaacaatcg gcaaggtatc tcaaaaaaac gggggatatg ttgtcagtat aaacatgacc
    21241 ccaatagaca atatgttaaa aagcttagat aatctaggtg gaaatggcga ggttgtgcta
    21301 gataatgcaa aatatcaggc atggaatgcc ggattctctg ccgaagatga aacaatgaaa
    21361 aataatcttc aaactttagt tcaaaaatac agtaatgcca atagtatttt tgataattta
    21421 gtaaaggttt tgagtagtac aataagctca tgtacagata cagataaact ttttctccat
    21481 ttctgaggtg cgccatgcat aatgtaaata atactcaagc gccaacattc ttatataagg
    21541 caacttcacc atcatcaaca gaatacagcg agttaaaaag caaaatatcc gatatccata
    21601 gttcgcaaac ttctctaaaa acaccagcat cagtgtctga aaaagaaaac tttgcaacgt
    21661 cttttaatca gaaatgtctt gattttttat tttcttcctc agggaaagaa gatgtgttaa
    21721 gaagcattta ttccaactca atgaatgcgt atgccaaaag cgagattctc gaattttcaa
    21781 atgttttgta ctccttagta catcaaaatg gtcttaattt tgaaaacgaa aagggacttc
    21841 aaaaaattgt cgcacagtat tcggaactaa ttataaaaga taaattatcc caagattctg
    21901 cctttggacc atggtcggca aagaataaga aactccatca attacgacaa aacattgagc
    21961 acagacttgc actattagca caacaacaca catctggtga agctttatca ttgggacaaa
    22021 aactcctcaa tactgaagta tcatcattta tcaagaataa tattcttgct gaattaaagt
    22081 taagtaatga aactgtttca tctctcaaac tagatgattt agttgacgca caggcaaaac
    22141 ttgcctttga tagtttgcgc aatcaacgta aaaatactat tgatagtaaa ggatttggta
    22201 taggtaaact gtcaagagac ttaaatacag tagccgtgtt tcctgagctg ttgagaaaag
    22261 tccttaatga tattttagaa gatataaaag attcgcatcc tatccaagat ggcctcccta
    22321 cacctcccga agatatgcca gatggcggac caacccccgg agccaatgag aaaacatccc
    22381 aacctgtaat tcactatcat ataaataatg ataatagaac ttacgataat agagtttttg
    22441 acaacagagt atatgacaat agctatcacg agaacccaga aaatgatgca cagtctccta
    22501 cttctcagac aaacgatcta ttatcccgta acggaaactc attactaaat ccacaaagag
    22561 cactagttca aaaagtaact tccgttctac cacactctat atcagatact gtccagacat
    22621 ttgcaaataa ttcagcttta gaaaaggctt tcaaccatac tccagataat tcggatggaa
    22681 taggttcaga cctgttaact acgagtagtc aagaaagatc tgcaaataac tctctttctc
    22741 ggggacacag gcctctgaac atacagaact cttcaaccac cccccctctc cacccggaag
    22801 gagtgacaag cagtaatgat aactcatcag atacaactaa aagtagcgct tctctttctc
    22861 atagagtagc ttcgcaaatc aataaattca actcaaacac tgattcaaaa gtacttcaga
    22921 ctgatttttt atcaagaaat ggagacacat atttaacacg ggaaacgata tttgaagctt
    22981 caaaaaaagt aacaaactcc ctaagtaatc ttatatctct cattggaact aaatcaggaa
    23041 cacaagaacg agagttacag gaaaaatcaa aggacattac aaaatccaca acagaacata
    23101 gaataaacaa caaattaaaa gttacagatg caaatataag aaactacgta acagaaacca
    23161 acgcagatac aattgataaa aatcatgcga tctatgaaaa ggcaaaagaa gtatctagcg
    23221 ccctcagcaa ggtattgtca aaaattgacg atacctctgc agaattactt acagatgata
    23281 tatctgattt aaaaaataac aatgatatta cagctgaaaa caataatata tataaagcag
    23341 caaaagatgt aaccacttcc ctatcaaaag tattaaagaa tatcaataag gattaaaata
    23401 tgataaaaga aaaaatatta tcaatagtgg ccttttgcta tggtatagcg tacagcaaac
    23461 tatctgaaga aactaaattt attgaagatc tttctgccga ttctctttct ctgattgaaa
    23521 tgctggatat gattagtttt gagttcaatc tacgtataga tgaatctaca ttagaacaca
    23581 ttattactat tggagatctt atttctgtag tcaaaaatag tacaaaatca atatagtaat
    23641 tgataagcat tttttcatct atggagctct cacatcagag ctccacaaga atattattct
    23701 tttatccaat aaagataaat tgcatcaatc cagctattaa aatagtaatt tttaagacta
    23761 ccgttgacta tcatcaacag ttacatacaa tcttgctcac actgcattta acttttgtca
    23821 atataaatca atatgaataa acagggtgtg atatggtgga tttgtgcaac gacttgttaa
    23881 gtataaagga aggccaaaag aaagagttta cactccattc tggtaataaa gtttccttta
    23941 tcaaagccaa gattcctcat aaaaggatcc aagatttaac cttcgtcaac caaaaaacga
    24001 atgtacgcga tcaagaatcc ctaacagaag aatcattagc cgatatcata aaaactataa
    24061 agctacaaca attcttccct gtaataggaa gggagattga tggtagaatt gaaattctgg
    24121 atggcactcg tagaagagca tctgcaatat atgcaggagc agatcttgaa gttctatatt
    24181 caaaagaata tatatctact cttgatgcca gaaaactagc aaacgatata caaacagcaa
    24241 aagagcatag catccgagaa cttggtattg gtcttaattt tctgaaagta tcagggatgt
    24301 cctataaaga catagccaaa aaagagaatc tgtctcgcgc gaaagtcact cgtgcctttc
    24361 aggcagcaag cgttccacag gaaataatat ctctatttcc aatcgcgtca gaacttaact
    24421 ttaatgacta caagatatta ttcaattatt ataaaggact tgaaaaggct aatgaatctc
    24481 ttagttctac actaccaata ttaaaggaag aaataaaaga ccttgataca aatttgcccc
    24541 cggacatata taaaaaagaa attttaaaca tcataaagaa aagcaaaaac agaaagcaaa
    24601 acccttcgct aaaagttgac tcgttattta tttctaaaga caaacggact tacataaaaa
    24661 gaaaagagaa taaaacaaac agaactctaa tatttacatt atctaaaata aacaaaacag
    24721 ttcagagaga aatagatgaa gctattcggg atataatttc tcgccatcta tcgtcttcat
    24781 aaacatcgaa cactgatgta aactgcccaa ttcagataat aaaaatcttc gttgaacttg
    24841 actttcagcc ggtggggtaa ttccgtcggc tatctgttat tcaatcccca cgcactgtgt
    24901 ttccttaaaa ttacggtgaa aactcatacc ctgctgacca cgagaaaatt atctggtgaa
    24961 attagtaata ttcatatcat tgaaaatgaa ttgtgcaact aagggatgcg gcatgatggt
    25021 caaaatttta tgtgaatatg agtgactggc cgaaaatatt cagcgtattt ttaatcgttt
    25081 caataaaaac agcgaggatt agcagtcaac gcttttacta ctcaattttt tttacatggt
    25141 gataaacaaa aaatccacca ttaacagact tcgttcacag tatcaaaaaa gctttaagaa
    25201 tatattagca gatttctcaa atataattac tgttttattc tcctaatctt cccataaaat
    25261 gaccgactta cattatttta taattataaa caagtttgat tttaaaatca tattaattat
    25321 taacaagaga tttatttttc taaccccaaa agttatgctt ggtcaaagag taacttataa
    25381 tatccatacc cctcataggg cttacagttt aaattgcggg tataaaatac atctattttc
    25441 aaataaaata aagcatactc caattacaac gagtgactaa aataaaaata tcatccaagt
    25501 caatcactgt ttgctctgca aaatatgtca gccttctcaa acttaccagc acttaaatat
    25561 ttaagcacac aatcattcct tgagggagtt cacttatttc aagagaaaca tttcgtcttc
    25621 ccacacaact aatccgccca atatagctaa atttgtactc agtaatatca tttgttatgc
    25681 cccccacccc aaaatattca agctccttca cagataaaaa acgacaattt acaaagcctc
    25741 attagttata actatggaaa tacctatttt agtatatcct ataaagttag aattacttct
    25801 tcttgcacct aattccatag ttgaaaaaca agaataattt tctcctacag ctggatccat
    25861 atagcttcca tcaggtctac acaacaccca gtggaggccg acaggaactc caataaagga
    25921 tacagcaaca gcttccaaaa ctctttggtt agaagataac acatctcgtt gatgtactat
    25981 attacatccc atgccaatta ataagtccct tgcatcggga taaataaaac taattacttg
    26041 cgaaaaaatg ttactttctt caacgacata agcattcaat ccaagaagcc tcgtagccat
    26101 gactatctta tcaggcatag aataaccggc atccgcgata ttatctttgt gtattctcgg
    26161 attataatta ccactagtta ttaagtataa atcccgctca caacgattat ccaaatcaag
    26221 agaactcttc cttgtcattt cggacataga ccctttgtat tgtggtattt tatcaacccc
    26281 taattccttt gcagcacaga gtaaactggc agctccacaa gaattgtcat acatctgctt
    26341 cctcgccaaa aaacaacctt gttcgtttac tctattctca tttaccctca catcagtctg
    26401 atgagatatc atatattctc ttggtatagc cccctgcaat aaaaccccat ataacataac
    26461 acctgtatga atacatcccc gtttgcaagg cttccgttgt tccgacatcg acgagtaatt
    26521 gcagcacagt attgcatcca gcaatgcgaa ctactatgag aattagctga ccgggtcctt
    26581 attccccgaa cgaactttaa atccaaaacc tcatactctt ttccgtcgtc atcacgtccg
    26641 tcaccataac aacctttcag catccaccct tttattaaga gttaatgtcc atcagcatca
    26701 gcaattaata ccgcctgata ctgacatatt tttgtcgtat ttacgtccat aatcggttat
    26761 tgtgatacag gtcacaccag cttatttgct attgttacaa taaccacctt ggttgagagc
    26821 tgttttttca actttgttat caactccccc aactccttgg tcagtaaggc tgccgaccga
    26881 gcacagtgaa tagatagtat ccgcaggtac caccatgctt acttaatact cagaaggcga
    26941 agtgctctcc aattaactct gccgcactat gcttataagc tgcttttcaa tgccgtctat
    27001 caacatgtcc aattcattca atctgttgta ctggtaatcc aacgcagtaa tgatatatgc
    27061 aggtattctt tcatccactc cagtaacgtt tcatgcaaag cattaattta cgcgctacgg
    27121 aacttcacca gttgactgag caaacgatgt gggaccagta ctgcctgcag ttcttctatt
    27181 gttacagcga tttctttacc aaacagctga accaccatca agataaagat agcctgaaca
    27241 ttcatcgcac cattttgtta ccaatggcaa aagatttaac gaaccgtgcc tgcatcaggc
    27301 ggacttttta tccctgcttt gttagctccc taaccttgta cagagagcct ccataagctc
    27361 ccatatcaag cagacatggt tcgcagttac tgacatacac cagaaaactc tgacagcaga
    27421 ctcacttatc ctccacttca ctcatatcct tattggtgag atgaacctga atgacatgtt
    27481 ttgcgacata aacgccaacc agtgtatatt tcacctgtag agattctaca atctgtagcg
    27541 ttatgcatct aatgttaggc tctatgatgt cagaaatctg cgagactcta caccttctgc
    27601 atttaccttt atttatcacc atgctcctca gttaacggag aacgtgttca ttgcattttg
    27661 tcacttacat ttcgcctttg gtatcggggg gatataggtt ccaacgttcc gcatgagaac
    27721 ctgaaccggc ttcacgccac cttgatacca gatgccgcac aggtagtcaa caagtgtcac
    27781 gaacggtgca atagtgatcc acacccaacg cctgaaatca gatccagggg gtaatctgct
    27841 ctcctgattc aggagagctt atggtcactt ttgagacagt tatggaaatt aaaatcctgc
    27901 acaagcaggg aatgagtagc cggacgattg ccagagaact ggggctctcc cgcaataccg
    27961 ttaaacgtta tttgcaggca aaatctgagc cgccaaaata tacgccgcga cctgctgttg
    28021 cttcactcct ggatgaatac cgggattata ttcgtcaacg catcgccgat gctcatcctt
    28081 acaaaatccc ggcaacggta atcgctcg
//
LOCUS       DAJTQQ010000051        27763 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000051 DAJTQQ010000000
VERSION     DAJTQQ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27763
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.051"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..630)
                     /gene="phnD"
                     /locus_tag="OO309_002340"
     CDS             complement(<1..630)
                     /gene="phnD"
                     /locus_tag="OO309_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5847459.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKT"
     gene            complement(655..1443)
                     /gene="phnC"
                     /locus_tag="OO309_002341"
     CDS             complement(655..1443)
                     /gene="phnC"
                     /locus_tag="OO309_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR5847460.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            complement(1576..2019)
                     /gene="yjdN"
                     /locus_tag="OO309_002342"
     CDS             complement(1576..2019)
                     /gene="yjdN"
                     /locus_tag="OO309_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR5847461.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(2177..2512)
                     /locus_tag="OO309_002343"
     CDS             complement(2177..2512)
                     /locus_tag="OO309_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR5847462.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            2914..5142
                     /gene="crfC"
                     /locus_tag="OO309_002344"
     CDS             2914..5142
                     /gene="crfC"
                     /locus_tag="OO309_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR5847463.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            5139..6017
                     /locus_tag="OO309_002345"
     CDS             5139..6017
                     /locus_tag="OO309_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR5847464.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            6281..7783
                     /gene="proP"
                     /locus_tag="OO309_002346"
     CDS             6281..7783
                     /gene="proP"
                     /locus_tag="OO309_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR5847465.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            7895..7984
                     /gene="pmrR"
                     /locus_tag="OO309_002347"
     CDS             7895..7984
                     /gene="pmrR"
                     /locus_tag="OO309_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR5847466.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(7960..9060)
                     /gene="pmrB"
                     /locus_tag="OO309_002348"
     CDS             complement(7960..9060)
                     /gene="pmrB"
                     /locus_tag="OO309_002348"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR5847467.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(9061..9729)
                     /gene="basR"
                     /locus_tag="OO309_002349"
     CDS             complement(9061..9729)
                     /gene="basR"
                     /locus_tag="OO309_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR5847468.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(9726..11369)
                     /gene="eptA"
                     /locus_tag="OO309_002350"
     CDS             complement(9726..11369)
                     /gene="eptA"
                     /locus_tag="OO309_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR5847469.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            complement(11473..12810)
                     /gene="adiC"
                     /locus_tag="OO309_002351"
     CDS             complement(11473..12810)
                     /gene="adiC"
                     /locus_tag="OO309_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR5847470.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(12947..13708)
                     /gene="adiY"
                     /locus_tag="OO309_002352"
     CDS             complement(12947..13708)
                     /gene="adiY"
                     /locus_tag="OO309_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR5847471.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(14033..16300)
                     /gene="adiA"
                     /locus_tag="OO309_002353"
     CDS             complement(14033..16300)
                     /gene="adiA"
                     /locus_tag="OO309_002353"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR5847472.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(16499..17407)
                     /gene="melR"
                     /locus_tag="OO309_002354"
     CDS             complement(16499..17407)
                     /gene="melR"
                     /locus_tag="OO309_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR5847473.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            17690..19045
                     /gene="melA"
                     /locus_tag="OO309_002355"
     CDS             17690..19045
                     /gene="melA"
                     /locus_tag="OO309_002355"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR5847474.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            19148..20569
                     /gene="melB"
                     /locus_tag="OO309_002356"
     CDS             19148..20569
                     /gene="melB"
                     /locus_tag="OO309_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR5847475.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(20708..21337)
                     /locus_tag="OO309_002357"
     CDS             complement(20708..21337)
                     /locus_tag="OO309_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR5847476.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(21460..23106)
                     /gene="fumB"
                     /locus_tag="OO309_002358"
     CDS             complement(21460..23106)
                     /gene="fumB"
                     /locus_tag="OO309_002358"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5847477.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(23184..24524)
                     /gene="dcuB"
                     /locus_tag="OO309_002359"
     CDS             complement(23184..24524)
                     /gene="dcuB"
                     /locus_tag="OO309_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR5847478.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(25095..25814)
                     /gene="dcuR"
                     /locus_tag="OO309_002360"
     CDS             complement(25095..25814)
                     /gene="dcuR"
                     /locus_tag="OO309_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR5847479.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(25811..27442)
                     /gene="dcuS"
                     /locus_tag="OO309_002361"
     CDS             complement(25811..27442)
                     /gene="dcuS"
                     /locus_tag="OO309_002361"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR5847480.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            27623..>27763
                     /gene="yjdI"
                     /locus_tag="OO309_002362"
     CDS             27623..>27763
                     /gene="yjdI"
                     /locus_tag="OO309_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HCR5847481.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
BASE COUNT         6638 a         6746 c         7245 g         7134 t
ORIGIN      
        1 ggttttcagc ttgtcgaggt tttcggtgtt gttggtggca acatccactt gcttgttggc
       61 gacggccagc gcgttggttt catgcccggc gttgacggtg cgcttgaagt cgctggcgga
      121 gatattgttt ttagcgaaga cgtagtaacc ggggacgagg aagccagagg tggagttagg
      181 atcgccattg ccgaaggtga gatctttccg cttcgccagc agatcgttca ggttgttgat
      241 cgggctgtct ttgttgacga tcaacacgct ccagtaaccc ggcgatccat ccgccgcgac
      301 cgtctgggcg aagacctggc cattggcgcg atccaccgct tccatcgccg acaggttgcc
      361 gtaccaggca atatccactt tattgaagcg catcccctgg ataatgcccg cgtagtccgg
      421 ggcaaagaag gcgttgacct tcacgcccag tttcttctcc atatcctgta aaaacggcgt
      481 ccattgcggt ttcaggtttt gctgtgattc cgttgaaata atgccgaaat tcagcgcctt
      541 ttcctgctct tcggcgtgtg ccggggttaa cagggtgctg aggctgaaca tgctggtgaa
      601 ggccagcgag gcaattatct tagcgttcat tcgttttcct caatgatggg gatatcaggc
      661 agctttcgcg ttctcttcga tgcggttaat gctgcggtag agatggtcaa aacgttcgtt
      721 atcaaacagt tggctgctgc cgtcgtagaa gacgtgcccc tgacgcaggg cgacgatgcg
      781 ttcgcagtag cgcagggcgt aatccacctg atgcagcgtg acgaccacgg tgatgccgtc
      841 gttctggttg atgtcgcgca gggtgtccat cacgatgcgc gctgattccg ggtccagcga
      901 ggcgatgggt tcatcggcca gaatcacttt cgcctgctgc atcagcgcac gggcaatcgc
      961 cacacgttgc tgctggccgc cggagagggt ggaaacgcgc tgatgggcaa aatgcaccat
     1021 gccaacgcgg gtcagcgcct gtaacgcgcg ctgtttctgc tcgccggtga accagctaaa
     1081 acaggtgcgc cagaacggcg tgctgccgag cgcgccaatc agcacgttct ccagtacgct
     1141 caggcggttc accaggttga attgttgaaa gatgtagccg gtgttggcgc ggcttttgcg
     1201 gatatcgcgg gccaggcggc cttcgcgctg gacagtgcgg cccagtagct cgatatggct
     1261 accggcggat ttatcgccgg taatcaaacc gcttaaatga cgtaaaaggg tggatttgcc
     1321 ggaacccgac ggcccaagca gagccaccat ttcactgtga tgaatgttca gatcaaccgc
     1381 atgcagcgct tgatgctgat tgaaggtttt ggcgagcttc tcgacacgga taatcgtttg
     1441 catgatgcag cctccctaaa aaagtggctc catcgtggcg gattattgtg acgatttggt
     1501 taattaaaag tgactaacag atgaagagtt aacgggaatt cgatgacagt gcgggagggc
     1561 gggcctcccg gcgggttatt gcgttggttg ttgtttgacg acattaatca tccacggtac
     1621 gccaaattta tcggtgactt tgccaaagcc atgcgcccag aaagtttcct gccaggccat
     1681 ttcgattttt ccgttagcgg caaggttatc aaaccagcgt tttccttctt cgacctgttg
     1741 cgaatcgagc accagcgtaa agccggagta gctggctttt cctgacggta tagcatcgct
     1801 catcatgatg tcgcttccgg caatgcgcac gttggcatga gcgatggcgg tatcgggaaa
     1861 ttgcattccg gaagggcagt tctcggcgct gtcctgcgct gatttcggca tttcgccgaa
     1921 gctgatttta tagagcagtt ccgcgcccaa cgtacgttga taataggcaa tcgcgtcgga
     1981 acagttaccg gcaaaagaga ggtagggact taacggcatg atggtgacct cagttaagag
     2041 aagccagtta agtgtagttc gaaatttata cagatgagag gctgcctgat aagacgcgca
     2101 agcgtcgcat caggcagtcg gcatcgttgc cggatgcggc gcgagcgcct tatccggctt
     2161 accgatcaca atacaatcag ttctttttca caaactcaga tttcagtttc atcggaccaa
     2221 aaccgtcgat tttgcaatcg atgttatggt cgccttcaac caggcggatg tttttcactt
     2281 tggtgccaat tttcagcatc gaagagctac ctttcacctt cagatcttta atgatggtaa
     2341 cgctgtcgcc gtcagccagc agattgccgt tagcatcttt aacgatcagc tcgtcgcttt
     2401 cctgtgcagg ttctgcgtcg ttccattcgt aggcacattc cgggcagatg tacatgccgt
     2461 tatcttcgta agtgtattcg gagttgcatt ttgggcagtg tggtaatgac atgtggattt
     2521 cctcaaaagt cagcagggca aaagcgccaa aaaacggcag attgccgaaa aaggccgcaa
     2581 ttatacacaa aatccctgag tttgtcgggg gtactgctga ttttagacga cgatcgtttt
     2641 catttgaaag gaaacgcaaa aagaaaataa tctttttgcc tctatttatt atttaagata
     2701 atgttaatgc tctacttgtt ctttttttat tcacatgata tttttatctt tctgacgttt
     2761 taaacgcatc agaagacgtt atttattctg ataaaaaaac aacacactaa atataaaatt
     2821 atttcttgca gtaattataa aagccgaaaa cagaactcaa aaacaatctg gctaaataaa
     2881 agtaacaaaa tttgctttaa ggaagaattt tctatgtaca cacagaccct gtatgagtta
     2941 agtcaggagg ctgaacgcct gttacagctt tctcgccaac agttgcagtt actggaaaaa
     3001 atgcctctct ctgtacccgg agacgacgcg ccacaactgg ctttaccctg gagtcagcct
     3061 aatatcgccg aacgtcacgc gatgctgaat aatgagttgt gtaaaatttc ccgactggaa
     3121 atggtgctgg caattgtcgg taccatgaaa gcagggaaat caaccaccat taatgccatt
     3181 gttggtacgg aggttctgcc taatcgtaat cgcccaatga ctgcgctgcc gacgcttatt
     3241 cgccatacgc ccgggcaaaa ggaaccggta ctgcattttt cacatgtcgc gccaatcgat
     3301 tgtttaattc aacaattaca acagcgcctg cgtgattgcg atattaagca tctgaccgat
     3361 gtgctggaaa tagataaaga tatgcgtgcg cttatgcagc ggatcgaaaa tggcgtcgct
     3421 ttcgaaaaat attatctggg tgcccagcct atttttcatt gtctgaaaag tttgaatgat
     3481 ttagtgcgac tggcgaaggc gctggacgtc gattttcctt tttctgctta cgccgccatt
     3541 gagcatattc ccgtgattga agtggagttt gtccatctgg cggggctgga aagttatccc
     3601 ggtcagttga cgttactgga tacccccggg ccaaatgaag ccgggcaacc gcatctgcaa
     3661 aaaatgctta accagcagct ggcacgcgcc tcggcggtac tggcggtgct ggattatacg
     3721 caactgaaat cgatctccga tgaagaggtc cgtgaggcga ttttggcggt ggggcaatcg
     3781 gtgccgctgt atgtgctggt caataagttc gatcaacagg atcgtaacag tgacgacgcc
     3841 gaccaggtgc gggcactgat ttccgggacg ctgatgaaag gctgtattac gccacagcag
     3901 atattcccgg tgtcgtcgat gtggggctac ctggcgaatc gggcgcgcta tgagttagcc
     3961 aacaacggta agttaccagc gccagagcaa caacgctggg tggaagattt tgcccatgcc
     4021 gcgctcggca ggcgctggcg tcatgccgat ctggcggacc tcgaacatat tcgtcatgct
     4081 gccgatcagt tgtgggaaga ttcgctgttc gcccagccaa ttcaggcgtt gcttcatgcc
     4141 gcttacgcta acgcctcatt gtatgctctg cgatctgctg cgcataaact gttgaattac
     4201 gcgcagcagg cgcgggaata cctggatttt cgtgcgcacg ggttaaacgt cgcttgtgaa
     4261 caattgcggc aaaatatcca tcaggtcgaa gaaagtttgc agctattgca actcaatcag
     4321 gcgcaggtga gcggcgagat taaacatgaa atcgagctgg ccctgacctc cgccaaccac
     4381 tttctgcgtc aacagcaaga tgcggtgaat gcgcagttag ccgccttgtt tcaggatgat
     4441 tcggagccgt taagcgagat tcgtacccgc tgtgagacac tgttacagac ggcgcagaac
     4501 accatcagtc gcgactttac gctgcgtttt gccgagatgg aatccaccct ttgccgggtg
     4561 ttaaccgatg ttattcgccc cattgagcaa caagtcaaaa tggaattgag cgagtcaggg
     4621 tttcgtcctg ggtttcattt tcctgttttt cacggcgtag ttccccactt caacactcgc
     4681 cagctgttca gtgaagtcat ttcgcgccag gaagcaacgg acgagcagag cacgcgttta
     4741 ggcgttgtgc gtgagacttt ttcgcgctgg ttgaatcagc ccgactgggg acggggaaat
     4801 gagaaatccc cgacagaaac ggttgattac agtgtgttgc aacgagcatt aagcgcagaa
     4861 gtcgatctct attgccaaca aatggctaaa gttctggcag agcaggtcga tgaatctgtt
     4921 acggcaggca tgaatacttt tttcgctgag ttcgcttcat gtttgacaga attacagacg
     4981 cgtttacgcg aaagtctggc tctgcgtcaa caaaatgaat cggtggtcag gctgatgcag
     5041 cagcaattgc agcagactgt gatgactcac agctggattt acaccgacgc tcagctgtta
     5101 cgcgatgata ttcaaacact tttcacggca gaacgatatt gaccaagacg ttactggacg
     5161 gccccggtcg cgtgctggag tcggttcatc cgcgcttttt agtggatctg gcgcagggcg
     5221 atgatgcccg ccatcctcaa gcccatcagc agcagtttcg tgaacgactg atgcaggaac
     5281 ttctttcgcg tgtgcagctt cagacatgga cgaacggcgg catgttaaat gcgccgctta
     5341 gcctgcgact gacaatggtg gaaaaactgg cgtcgatgct ggatcccggt catctggcgc
     5401 tgacgcagat cgcgcagcat ctggcgctgc tgcaaaaaat ggatcaccgc cagcactctg
     5461 ctttcccgga gctcccccag caaattgccg ccttgtatga gtggttttca gcccgttgtc
     5521 gctggaagga aaaggcgtta acgcaacgag gcctactggt gcaggcaggt gatcagagcg
     5581 agcaaatttt tacccgctgg cgtgctgggg cgtataacgc ctggtcgttg cctgggcgct
     5641 gttttatcgt tctggaggag ttgcgctggg gtgcatttgg cgatgcctgc cgtctgggaa
     5701 gcccgcaagc ggtggtgttg ttgctgggtg atttgcgcga gaaagcgaca caacatctgg
     5761 cagagagtat caatgcggca ccgactacgc gtcactatta ccatcagtgg tttgcctctt
     5821 cgaccgttcc gacgggcggg gatcatgctg attttttaag ttggctggga aagtggacca
     5881 cggcagataa acaacccgtt tgctggtcag tgacccaacg ctggcaaact gtcgcgctgg
     5941 ggatgccacg actctgttca gcgcagcgtt tggtgggggc aatggtcgag gaaatcttct
     6001 ctgtaaattt ggcgtaaata atcagttaca tcaatgagtc ctaaacgaaa tccatgtgtg
     6061 aagttgatca caaatttaaa cactggtagg gtaaaaaggt cattaactgc ccaattcagg
     6121 cgtcaactgg tttgattgta cattccttaa ccggagggtg taagcaaacc cgctacgctt
     6181 gttacagaga ttgcatcctg caattcccgc tccccttttg cggccgtcgc gctgattttt
     6241 ctggcgtttg cggaaatggg ccaactctgc gaggaaagct atgctgaaaa ggaaaaaagt
     6301 aaaaccgatt acccttcgtg atgtcaccat tattgatgac ggtaaactgc gtaaagccat
     6361 taccgcagca tcactgggta atgcaatgga atggtttgat tttggtgttt atggttttgt
     6421 tgcttacgca ttaggtaaag tttttttccc gggggctgac cccagcgtgc agatggttgc
     6481 tgcacttgcc actttctccg ttccctttct gattcgaccg cttggcggac tcttctttgg
     6541 tatgttgggc gataaatatg gtcgccagaa gatcctcgct atcactattg tgattatgtc
     6601 gatcagtacg ttctgtattg gcttaatacc gtcctacgac acgattggta tttgggcacc
     6661 gattctgctg ttgatctgta agatggcaca aggtttctcg gtcggtggtg aatataccgg
     6721 ggcgtcgata tttgttgcgg aatactcccc tgaccgtaaa cgtggcttta tgggcagctg
     6781 gctggacttc ggttctattg ccgggtttgt gctgggtgcg ggcgtggtgg tgttaatttc
     6841 gaccattgtc ggcgaagcga acttcctcga ctggggctgg cgtattccgt tctttattgc
     6901 tctgccgtta gggattatcg ggctttacct gcgccatgcg ctggaagaaa ctccggcgtt
     6961 ccagcagcat gttgataaac tggaacaggg cgaccgcgaa ggtttgcagg atggcccgaa
     7021 agtctcgttt aaagagattg ccactaaata ctggcgcagc ctgttgacat gtattggtct
     7081 ggtaattgcc accaacgtga cttactacat gttgctgacc tatatgccga gttatttgtc
     7141 gcataacctg cattactccg aagaccacgg ggtgctgatt attatcgcca ttatgatcgg
     7201 tatgctgttt gtccagccgg tgatgggctt gctgagtgac cgttttggcc gtcgtccgtt
     7261 tgtgctactt ggtagtgttg cactgtttgt gttggcgatc ccggcgttta ttctgattaa
     7321 cagtaacgtc atcggcctga tttttgccgg gttactgatg ctggcggtga tccttaactg
     7381 ctttatgggc gttatggctt ctaccttgcc agcgatgttc ccgacgcata tccgttacag
     7441 cgcgctggcg gcggcattta atatttcggt gctggttgcc ggtctgacgc caacactggc
     7501 ggcctggctg gtcgaaagct cgcagaatct gatgatgcct gcctattacc tgatggtagt
     7561 ggcggtgatt ggtttaatca ccggcgtaac catgaaagag acggcaaatc gtccgttgaa
     7621 aggtgcgaca ccggcggcgt cagatataca ggaagcgaag gaaattctcg tcgagcatta
     7681 cgataatatc gagcagaaaa tcgataatat tgaccacgag attgccgatt tgcaggcgaa
     7741 acgtacccgc ctggtgcagc aacatccgcg aattgatgaa taagctgaaa cggatggcct
     7801 gatgtgacgc tgtcttatca ggccaattga actcttaagg ttcacttaat ctctgacgcg
     7861 catactctcc tccaggttaa cggaggagag tgcaatgaaa aaccgtgttt atgaaagttt
     7921 aactaccgtg ttcagcgtgc tggtggtcag cagctttctt tatatctggt ttgccacgtt
     7981 ctgatctttc ttcagccgta cccaggcccg cgtgccggaa gtctcttgcc ggttttgcag
     8041 gaaaaactgc ccgtgatgca actgtgtaat gcggctgaca atacttaacc ccagaccaat
     8101 cccgccataa cggctgtcca tacgtacaaa cgctttactc aactccccgc atttactctc
     8161 atcaatacct ggtccttcat cttcaactgc catgaccgct ccgccatctt cttgcagctt
     8221 aatcataatg ttgctgcctt gcgggctgta acgatgggcg ttttctacca ggtttcgcaa
     8281 taacatccgc agcagggttg catcaccctg aacggtgatg tcggcggcgc tctctggcaa
     8341 tagcagggtt tgctgtcgct ggtcgagcat ggtactgagt tcgtcatacg aggggagaat
     8401 gacatcttcc agcagtttta catgttgata attaccggaa gaaaatgact gtccggcacg
     8461 cgccagttgc agcagctggg agacgctctc catcatctga tcaagccgtg ccactaacgg
     8521 tgctacatca atgtgatgcg ttttcgccag cagttccaga tgcaaacgca cccccgccag
     8581 tggcgttcgc agttcgtgcg cgacgtcagc ggtaaacaac ctttcgttat ccagcgtgct
     8641 ggtcaggcga ctgaccagat cgttcaacgc cgaaaccacc gcttcgattt cgagggtggc
     8701 gctgtgaatg gcaatgggcg ttaagttgtc ggcggtgcgc gcttccagct ctttttgcag
     8761 ctccgccagc gggcgggtga tgcggcgtac cgcctgatag cagataaata gcgtcaggct
     8821 gaccataaag acgccgggga caatcaggct ggcgaccgcc tcgcggatct cacgcatgat
     8881 gtggcgatcg ttgttgcgat tgtcgcgtag cgcctgctca aacagctgaa tctgctcggt
     8941 actttcatgc catagccaga agacgctgat cagctcaaac accaacaaaa tggccccgat
     9001 ggtcaatatc agccgttggc gcagcgatat aggtcggcgc agaaaacgca tcagattcaa
     9061 ttagttttcc tcattcgcga ccagcatata gccaaagccg cgcacggtgc ggatacgggc
     9121 tttgcccact ttgtcgcgca gattgtggat atgcacttcc agggtgttgg tcgagggttc
     9181 attgtcccag ttatagatgt cgttgtagag aatttcccga tgtaccggac tgcctgcttt
     9241 gagcattaac cgtgacagca gagcatattc tttgggcgtc agaatcaact cttcaccgcc
     9301 catccatacc tgacggcgac ccatattcag cgtcagattg ccaacaatca gctcactttc
     9361 gccctgatta ttatggcgtc gtagcagggc gcggatacgg gcgtgtaact cttccagcgc
     9421 aaaaggcttc accagatagt catcggcacc gacatccagc ccggcgattt tgtcggtcag
     9481 cgtatcgcga gcggtgagga tcagtaccgg cagggtgtat tttttctgcc ggatacgggc
     9541 gagaaaatgc agtccatctt cgtcgggtaa ccctaaatcc agtaccacca ggctgtaatg
     9601 accgtcctca aggctttgtt ccgccatccg cgcggttgtc acgccatcgc acgcgtagcc
     9661 ttcggtttgc gccgccagaa tcagtccctg caataacagc gtatcgtctt caacaatcag
     9721 aattttcatt tactcactct cctgcaagtt tgcagaatat catccgcggc ctggtaatac
     9781 ttcgtctcaa cgccagttaa tcccaatagc gtggagaata aattgtcttg tgaatagtgt
     9841 tgcgtttgcg cctgtttttg caggcagttc tggtcaacct gataccgctt ttgataatcc
     9901 tccgacagcc acagcagcat tggcacctgt ttttggctat ccggggcgat ggcataaggc
     9961 agaccgtgca gatagatgcc attttcacct aacgattcac cgtggtcaga aagataaacc
    10021 aggctggtgg taaatttatc ctgatgttct ttaagcagat taatcgcttt atcaacaata
    10081 tagtcgacgt aaaccagcgt gttgtcgtaa gtgttcacca gttgctcttt ggtacaggtc
    10141 tggatctcat tggtgtcgca ggttggggta aatttcctga actgcggcgg atagcggttg
    10201 taataggtcg gaccgtggct gccgatggtg tgtaagacaa tcacgccatc accctgcagg
    10261 ttattgatgt actcttccag cccgtggaac agcacttcgt catagcattc gccgttgatg
    10321 cactgatcag gcagattcag cgcggtgacg ttctggtgag gcacgcggtc gcaggcacct
    10381 ttacagccgc catcgttgtc attccacagc acgttgatgc ccgctcgctg aatgatatcc
    10441 agcacgcctt cctggtgctg tgccagctct tctttgtagt gctcacgcgg catatccgag
    10501 aacatgcacg gtactgaaac tgccgttgcc gtgccgcaag atgcggtatt agggaaatag
    10561 accacgttat ctttcgccag ccgcgggtta gtttcacgcg ggtagccgtt gagggagaag
    10621 ttctccgccc gcgaggtttc gccgacaatc aggatggtca aatttttacg tttttcgttc
    10681 tgcattaacg ggttgcggtg cgcgtcttca ccaattcgca ccagcggcag atttgccagt
    10741 cgctgatggg agtaccatga ccagctggca acaatgctgt tagaggggct taaggatttc
    10801 accagctctt tgttattgcg gaacaacgag gcgtagtctt tataaaacag tgcggcgacc
    10861 agcaaaatca gcagtacaga aaccagaata ttggctccac ggaaaagaac actgcgcaga
    10921 cgcgaggtgg caggtttgat ttttagccag caggcaatca gctcagcaag cacgccgctg
    10981 aatcccagcg ttaataacat ttgcggtgtc atcagcgcat aactttctgc tggagtggta
    11041 tcaataatat tggcaatcat cgagcggtcg atgacgatgc cgtaagtcat tatgaaatat
    11101 tgtgcagccg cgccaatcag aataaacagg caggccagtg gtcgattaag ccataagaaa
    11161 gagcttagtg tcaggacaat attaatcacg ctgaaagcga caaccggcat cgacaagaaa
    11221 accagtacgt tatgcagcga atccagcggc agcgcctgca acacctgttt aaaaaaggca
    11281 atattcaggc agatagagat ataaaaagcg gccaacaata gccaggcgag taaattcaaa
    11341 gagggtcttt ttagtaggcg cttcaacatc acggtgtttc catcgaacaa agtgcgcata
    11401 tgctcgcaaa gcaaaattaa gccaacctta agttcttaag gttggctttt atgtttgctg
    11461 gattaagggg aattaatctt tgcttattgg tgcatctaag ggatacgggt ttttatgtag
    11521 ccggttgtaa ttcagcgcat acatggcggt gatgaccatc agggtgacaa atgaccacat
    11581 aacctctttc gccccggacc ccaccacggc ccaaatgcag tagaggaagg caatggtagt
    11641 aacagccaga tatgccgggc gtgctttacc aaagtgaccg tggccgagca gcagtaacgc
    11701 cgcacaggtg taaagatatg gcaccagtgt aaagatgacc gagacggaag aaaccagacc
    11761 gaactctttg gtcgcgtttg gtgaaatgct gctgagctgg aagatggtca tcaaaatacc
    11821 gacgataatc agccccgcca ctggcgtacc cgctttattt acacgggcaa aaatcggtgg
    11881 gaacagtccg tcatcggcag cggctttcgc cgtttgaccc gccagcaacg tccagccgcc
    11941 cagtgaacct aagcaacccg cagctgcgca gaaggaaaca atggccccag cggtgtcacc
    12001 caacgccatc cgtgcggcat caccgaatgg cgaggcagaa acgcgcagtg cggcattagg
    12061 gatcatcccc ataatcgcgg tggtggagag cacatagcaa acggcggcaa tcaatacccc
    12121 accaatggtg gcgataggga cattgcgttt cgggtttttc accacacctg cggcaacgga
    12181 ggcactttcc acaccgatga acgaccacag cgtaacgtta agggtacttt gaatcgcacc
    12241 gaaggtgccc aggccgctga cgttccatgc cgccatatag gtttcaccac ggaaccagaa
    12301 ccagccaaat acggcaatcc cgacgatggg gatcagcgcc agcacagtgg caacggcctg
    12361 cacgcgggtg atcattttcg gaccgacaat gttcagcagg acgaagatcc acagcaccac
    12421 gacgcaggtg atggttaata ccaatggatc tttcagaatc gggaagaagt aacttaaata
    12481 tcctacgcca atgaccacca tggcgatatt gccgatccag caggccagcc agtagaggac
    12541 gttggtttga taaccgagaa acgggccaaa gcagcggcgg gcgtaagcgt aagaaccacc
    12601 aggacttggg tcgaggaacg acattttggc gtataccatc gagagcccca gcgcaccgat
    12661 aatcgtcacc aaccatccat aaatggcaat cccgccagtg gaggccaggt ttgcaggtaa
    12721 cagaaaaaca cctgacccca taatattccc cgacaccatc agggtgacgg ggattaagcc
    12781 cactttgtga gcatcagcat ccgaagacat aattaaactc ctgcgaaggc gagcttcgtg
    12841 acaataaatt acgtcatatc atacgcctgc attcgatagt tgaattatta tcgaccgggt
    12901 tatcgctgat gccgttatta atatagctct tcgttaaggt taaaaatcag gcgacagtgc
    12961 gttctctgtg ctgactaaca taatgcagcg gcgtcatacc gtagaagtct ttaaagacag
    13021 aaataaagta cgacgtactg ttgtagccgc aggactgtga tacctgagag atatttttac
    13081 cgtccatcat taattcattt acggcataac gcatgcggca ggtggtgatt atttggctat
    13141 aactggtgtt ttcgcttttc aactttttct ttaacagact tgggctaaga cataaacaac
    13201 tggctaccat actaagattc cagtctttgt gaatatcgct ttcaataatt tgataaacgc
    13261 tgtcacttac acaattacgt aacatataca tcattaatga aaggaatttt ttactgtcga
    13321 gaaaacggga tagcaccgtg aataataatg cgcgtgttcg ttccttttct gactcagttt
    13381 ctgcgggcat catgctgtgt tgcgcggcaa cgcgaaagac gtctggcgtc aggcatggca
    13441 gggtgagtat gggcgtagcg ctacgttgcc atactggtat ttgcgagaga tctttattca
    13501 gaaaccggag gtaatctttg atgatgtcgt gactaatatg agcaaccaaa gtgttgttaa
    13561 gagaggagac gtcgataata ttattttcac aatttaataa cgccatatgg ttagctttaa
    13621 ggctaatagg ttctttccca ttcaccctta tccagacatc tttttcagtc aataaaacaa
    13681 tacaaggttg gtcgctgcaa atcctcatac atgtactcct gagtgcgaat aaaagaccag
    13741 ttctcgctaa agcaaagcga taccgggaaa tggttatata attgcatgat gaatataaac
    13801 atgcataaaa ataacgagtt ttactaatat tacaaatttt tatagtaatt tctcatgtac
    13861 atattttttt acaggataaa taacaggcaa aaaaaagcgt ggggtggggc cgcgcaaaaa
    13921 gtatgacatt tttgtttgca gcaattttta tgaaaacgtt gccatactca aaaggcctaa
    13981 ggccaggcgg cgtaatgtta tttaaacaat tacgccttca gcggaatagt ggttacgctt
    14041 tcacgcacat aacgtggtaa ataccgtcaa taatttcagt cccttcagtt tcgtgttcaa
    14101 atctagggaa atggtggtcc caggattgca gcgagcgtaa ataacttact tgcggactgt
    14161 ttttatcgcc gaagttttca ccagacagca gcatcgggat tcctggcgga tacgggataa
    14221 ctgagtttgc cgcgatgcgt cctggcagat tttcaatgga taccagttcg acattgttgt
    14281 cgacaatcgc gttgtacgct tcacgcgggg tgatttccgc caccggcagg ccggaatagg
    14341 cttcgttcaa ccgtgcgcca gggttgtttt ctttcagcca ggcaaacatg gtgtcaccca
    14401 gatcgtgaat ccccatgttc gcgtaagtgt caggatattg ttcaacaagt tccggcatca
    14461 cctgcgccag cggtgtgttg gcgtcatagt ggtgtttgaa ggagcaaagg gtgttaacca
    14521 gagtccccca tttcccacgg gttacgccca tagagaacag gaacataatt tggaagtcag
    14581 tggtgcgggt aggcacaatg ccgtggcgac caagccaggc agtgaccagc gccgccggaa
    14641 caccggtttc ttccagttca ccatcttcac ccattcccgg agcaaggatg ctgactttaa
    14701 ttgggtcgag catactccag ttatccggaa tatctttgaa gccgtgccag ctttcgcccg
    14761 gatgcattac ccagcagtcc tgaacggtgg tcagcagttt ggttggtgcg tcagcaaagt
    14821 cataggtttt gccggtttgt gggtcggtga cgacttcttt gttccacggt ttgaagaacc
    14881 agctaccgtc agcggtgaac tctttatata gccgcgccat cgcctgacgg aaatcaaccg
    14941 cttcgtcaat cacttcctgt gtcagtgaca ggccgctgtt gccgtccatc atcgacaccg
    15001 ccacgtcgtt ggatgcgcag atggcataca gcggggaggt ggtggcatgc atcatgtagg
    15061 cctggttgaa gcgggagaag ttaatcgccc cacgaccttc acgtacatga atataagaag
    15121 cctgtgacag cgcattcagc agtttgtgga tggagtgggt ggcgaaaacg gtaggaccgt
    15181 tgtgatcgcc aggttcgccg cgcatggcat agtgatcggc atagatcggg ttgaaacgtg
    15241 catagccgta ccaggcttcg tcaaagtgca gacgatcgga ggttttttcc agcagatcct
    15301 gcgcttcttt agcgttataa cacacgccgt cataggtgca gttggttacc acgcagtaag
    15361 acggtttttg cccggctttg tctttggtca gcgggctttc actgattttc ttttgcaagg
    15421 tttcaggttg catttcctgc ggatagattg gcccgataat gccgtagcgg ttgcggcttg
    15481 gcaccatata gaccggtttc gcgcctgtca gcatcaaacc ttgttcgatg gatttatggc
    15541 agttacggtc aacgaccacg acatcgttat cggtcatgca agcctgcatg atggtgcggt
    15601 tagagccgga agtaccgacg actaccgacc aggagcgatc ggcaccaaat acgcgtgcgg
    15661 catatttttc gctttcgcca aatgcgccag tatggtcaag caaagaaccg agggaagttc
    15721 gttcgatgcc catgtcggta cggaacagat tttcaccata gtagtcatgg tagaaacgtc
    15781 cggcgggtgt tttggtaaaa ccaacgccgc cctggtggcc tggcgctgcc caggaatatt
    15841 catggatgtc actatatttc atcagcgcgc tgaacagtgg cggcaacagc tgctggcggt
    15901 agcgggtcat cgcggcaacg gcgcgtccgg cgataaagtc ggcggtatct tccagaatcc
    15961 aggcgaattc atcgacaagc tccagcagat cccggtccat tgcggcgagg gctttttccc
    16021 gatcgcccaa caggaagacc ggcacgtttt gttggcgctc atgaagctta ccgatcaatt
    16081 gtctgacgtt ttgatgttcg tccggatgct ccatttgata gctgaacatc aggcagtcaa
    16141 tggcttcgtt tgaagagaga atggcaaaac catcatcaaa ggaggtggat ttaatcacgg
    16201 taacattttg ctggcttaaa gcatctgcca gacgctcaac ggcgttaccg acccaggtgt
    16261 cttgatggag aaactcgctt tcaacaatta atactttcat cattgcttac ccggttatga
    16321 aggaatcttc gttgcgggca agtatcttcc ggccttttcg ggctgtaaag cgcgtgaaaa
    16381 acaaacattt ttgaaatatt tagttttata aacaatacgt tatgtgatta ttttaaatta
    16441 ttttcactgc ttattatcga cggctaaact atttttttgg ctgatactga tatcgtcttt
    16501 agccgggaaa cgtctggcgg cgctgttggc taagtttgcg gtattgttgc ggcgacatgc
    16561 cgacatattt gccgaacgtg ctgtaaaaac gactacttga acgaaagcct gccgtcaggg
    16621 caatatcgag aatactttta tcggtatcgc tcagtaacgc gcgaacgtgg ttgatgcgca
    16681 tcgcggtaat gtactgtttc atcgtcaatt gcatgacccg ctggaatatc cccattgcat
    16741 agttggcgtt aagtttgacg tgctcagcca catcgttgat ggtcagcgcc tgatcatagt
    16801 tttcggcaat aaagcccagc atctggctaa cataaaattg cgcatggcgc gagacgctgt
    16861 ttttgtgtgt gcgcgaggtt ttattgacca gaatcggttc ccagctagag aggctaaatc
    16921 gcttgagcat caggccaatt tcatcaatgg cgagctggcg aatttgctca ttcggactgt
    16981 ttaattcctg ctgccagcgg cgtacttcaa acgggctaag ttgctgtgtg gcaagtgatt
    17041 tgatcaccat gccgtgagtg acgtggttaa tcaggtcttt atccagcggc caggagagaa
    17101 acagatgcat cggcagatta aaaatcgcca tgctctgaca ggttccggta tctgttagtt
    17161 ggtgcggtgt acaggcccag aacagcgtga tatgaccctg attgatattc actttttcat
    17221 tgttgatcag gtattccaca tcgccatcga aaggcacatt cacttcgacc tgaccatgcc
    17281 agtggctggt gggcatgata tgcggtgcgc gaaactcaat ctccatccgc tggtattccg
    17341 aatacagcga cagcgggctg cgggtctgtt tttcgtcgct gctgcacata aacgtatctg
    17401 tattcatgga tggctctctt tcctagaata tcagaattat ggcaggagtg agggaggatg
    17461 actgcgagtg ggagcacggt tttcaccctc ttcccagagg ggcgagggga ctgtccgagc
    17521 atcatgaggc cgaaaactct acttttcagg taatttattc ccataaactc agatttactg
    17581 ctgcttcacg caggatctga gtttatggga atgctcaacc tggaagccgg aggttttctg
    17641 cagattcgcc tgccatgatg aagttattca agcaagccag gagatctgca tgatgtctgc
    17701 acccaaaatt acatttatcg gcgctggttc gacgattttc gttaaaaata ttcttggtga
    17761 tgtgttccat cgcgaggcgc tgaaaacggc gcatattgcc ctgatggaca ttgaccccac
    17821 ccgcctggaa gagtcgcata ttgtggtgcg taagctgatg gattcagcag gggccagcgg
    17881 caaaatcacc tgccacaccc aacagaaaga agccttagag gatgccgatt ttgtcgtggt
    17941 ggcatttcag attggcggtt atgaaccttg cacggtgact gatttcgagg tctgtaagcg
    18001 gcatggtctg gaacaaacca ttgccgatac gttggggccg ggcggtatta tgcgcgcgct
    18061 acgtaccatt ccgcatctgt ggcaaatttg cgagaacatg acggaagtct gccccgatgc
    18121 caccatgctc aactatgtta acccaatggc gatgaatacc tgggcgatgt atgcccgcta
    18181 tccgtatatc aaacaggtcg ggctgtgcca ttcggtgcag ggaacggcgg aagagctggc
    18241 gcgtgacctc aatatcgacc cggctacgct gcgttaccgt tgcgcaggta tcaaccatat
    18301 ggcgttttac ctggagctgg agcgcaaaac cgccgacggc agttacgtga atctctaccc
    18361 ggaactgctg gcggcttatg aagcagggca ggcaccgaag ccaaatattc atggtaatac
    18421 tcgctgccag aatattgtgc gctacgaaat gttcaaaaag ctgggctact tcgtcacgga
    18481 atcatcagaa cattttgccg aatatacgcc gtggtttatt aagctaggtc gtgaggattt
    18541 gattgagcgt tataaagtac cgctggatga gtacccgaaa cgctgcgtcg agcagctggc
    18601 gaactggcat aaagagctgg aggagtataa aaacgcctcc cggattgata ttaaaccgtc
    18661 acgggaatat gccagcacaa tcatgaacgc tatctggact ggcgagccga gtgtgattta
    18721 cggcaacgtc cgtaacgatg gtttgattga taacctgcca caaggatgtt gcgtggaagt
    18781 agcctgtctg gttgatgcta atggcattca gccgaccaaa gtcggtacgc taccttcgca
    18841 tctggccgcc ctgatgcaaa ccaacatcaa cgtacagacg ctgctgaccg aagctattct
    18901 tacggaaaat cgcgaccgtg tttaccacgc cgcgatgatg gacccgcata ctgccgccgt
    18961 gctgggcatt gacgaaatat atgctcttgt tgacgacctg attgccgccc acggcgactg
    19021 gctgccaagc tggttgcacc gttaaaacgc gactaaacgc tactgcgccg ggggatttat
    19081 tccggcgcac acctctgacg ataccaataa cagaaggcgg gcgttggtaa cagcgacccg
    19141 ataccctatg agcatttcaa tgactacaaa actcagttat ggatttggag cgttcgggaa
    19201 ggattttgcg atcggcattg tgtatatgta cctcatgtat tactacaccg atgtcgtcgg
    19261 gctgtctgtg ggtttggtcg gtactttgtt tctggtggcg aggatctggg atgctattaa
    19321 cgatccgatt atgggatgga ttgtaaatgc tacgcgatcg cgatggggta agttcaaacc
    19381 ctggatcctg atcggtacgt tggcaaactc tgtaatctta tttctcctct ttagtgcgca
    19441 tctgtttgaa ggtactactc agattgtctt tgtttgcgtg acctacatcc tctggggcat
    19501 gacttacacc attatggata ttcccttctg gtcgctggtt ccaaccatca cgctcgataa
    19561 acgtgaacgc gaacaactgg ttccttatcc gcgttttttt gccagtctgg caggctttgt
    19621 tacggcaggt gtgacgctac catttgttaa ttatgtcggc ggtggcgatc ggggatttgg
    19681 ctttcagatg ttcactctgg tattgatcgc cttttttatt gtttcaacca tcatcactct
    19741 gcgcaatgtg catgaagtct tttcgtcaga caatcaatcg tctgctgaag gaagccatct
    19801 gacacttaaa gccatcgttg cgcttattta taaaaacgat cagctttcat gcctcttggg
    19861 tatggctctt gcttataatg tagccagcaa cattattacc ggatttgcta tctattattt
    19921 ctcatatgtt atcggtgatg cggatttgtt cccctattat ctgtcgtatg cgggagctgc
    19981 taacctggtg acgttagtat tcttcccacg cttagttaaa tcattatccc gacgcatttt
    20041 atgggccgga gcatctattc ttccggtgtt aagctgtggt gttctcctgt taatggcatt
    20101 aatgagctat cacaacgtcg tcctcattgt gattgcgggt attttgctga atgtgggaac
    20161 ggcgcttttc tgggtattac aggtcatcat ggtggcagat accgttgatt acggtgaata
    20221 taaactgcac gtacgctgtg aaagcatcgc ttactccgtg cagactatgg tggtgaaggg
    20281 cggttcagcc tttgcggctt ttttcattgc ggttgtgtta gggatgattg gctatgtacc
    20341 gaatgttgaa cagtctacgc aagccctatt aggtatgcag tttattatga ttgctctacc
    20401 gactctgttt ttcatggtaa cgctgattct ctacttccgt ttctatcgcc tcaatggcga
    20461 cacgctgcgc aggatccaga ttcatctgct ggataaatat cgcaaagtac cgcccgagcc
    20521 tgttcatgct gatattccgg tcggtgcagt gagtgatgtg aaagcctgac gtggacaata
    20581 gctaacgaaa atggcctgat gcgatatgtt tatcaggcca cgtcttaatg taatgctttg
    20641 gatttgcaaa ttttgtagac cggataagga attcacgccg catccggcat caacaaagtg
    20701 cacgttgtta tccagttatc aagccatagc gccgtagctg gcggcaatga aagcgagcga
    20761 gcaatatcac tgtcgttaat gcgccaagca gcgcgcagaa catatcggat tgcgtgtccc
    20821 actggtcgcc ctgggtaccg agaaaatcat ccgctccctg gcccatcgcc agtgccgccc
    20881 accactcgat taattcatac atcgcgctta tcgccagcgc cacgcaacag accagaaacg
    20941 ccaccatttt acgtccgcgc acgtacatcc cgcgaacgag aatttctcgt gccaccagtg
    21001 caggcaccag cccctggaaa aagtgcccca gcttgtcata cggattacga ctcaacccca
    21061 gccattcctg cacctcaaaa ccaacgggga ctttcgcgta ggtgtactgc ccgcctacca
    21121 tcaggatgat ggcgtgaaag aagatgagcg tatagagcag cagcgttaac gggtaacgtc
    21181 tggcggtggc aagtagtagc ggcacgacga taatcaccgg tgtcacttcc agcagccagg
    21241 tgagtttgtc atgggccgaa atgccggtat aaatcaggat taacgttagc gccagtgcgc
    21301 cagtgttaag aattaacggc ttgagtgtgc gggtcatggt gagttcgcaa gtcagggaaa
    21361 atactgacta ttcaccagca agcatgaaat tacaatcgca aaagaggtgg cccaggggga
    21421 tcacctggca gcatgctgcc aggcgctggg ccgaagaggt tacttagtgc agttcgcgca
    21481 ctgtttgttg acgatttgct ggaagaagtc gttacctttg tcatcgacca ggataaacgc
    21541 cgggaaatct tctacttcga ttttccagat agcttccata cccagctccg gataagcgac
    21601 gcattccaga tgcttgatgc tctgctgcgc cagtaccgcc gccggaccgc cgatgctacc
    21661 gaggtagaag ccgccgtgtt tatgacacgc gtcggtaacc tgctggctgc ggttgccttt
    21721 cgccagcatg atcatgctgc cgccgtggga ttgcagcaga tccacatagg agtccatacg
    21781 acctgcggtg gttgggccaa gtgaacctga tggataaccg gcaggggttt tcgccggacc
    21841 cgcgtagtag atcgggtgat ctttgatgta ctgcggaagt tctttaccgg cgtcaatcag
    21901 ctctttcagc ttggcgtgtg caatatctcg acccacaata atggtgccag tgagcgacaa
    21961 acgagtggaa accggatatt gcgaaagctg ggcgaggatc tctttcatcg ggcggttaag
    22021 gtcgaccttc accgcttcgc cttcaccggc ctgacgcagt tcttgtggaa tgtactgacc
    22081 tagattgtgt tccagttttt cgatccagat accttcgcgg ttgattttcg ctttaatgtt
    22141 acggtcagcg gagcaggaga cgcccatgcc taccgggcag gatgcgccgt gacgtggcag
    22201 acggataacg cgaatgtcgt gcgcgaagta tttaccgcca aactgcgcgc caagaccgag
    22261 tttctgggcc tcttccagca gttcctgttc cagctggaca tcgcggaacg cctgaccatg
    22321 ttcgttccct tccgtcggca gttcatcgta atagtgagcg cttgctaatt tgacggtttt
    22381 caggttgttt tccgcagacg taccgccaat cacaaacgcg atatggtacg gcgggcaggc
    22441 tgcagtaccg agggtacgca ttttctcgac gaggaagttt ttcagtttgc cgggagtcag
    22501 cagggctttg gtttcctggt agagatacgt tttgttggca gagccgccgc ctttcgcaac
    22561 gcaaaggaat ttgtactcat cgccatctac cgcgtacagg tcgatttgcg caggcaggtt
    22621 agtgccggtg ttgacctctt tgtacatgtc cagcgccgcg ttttgcgagt agcgcaggtt
    22681 atcttcgata taggtgttat agacgccttt cgacagcgct tcttcatcac cgccaccggt
    22741 ccacacgcgc tggccttttt taccgacgat gatcgcggtg ccggtatcct ggcaggtcgg
    22801 cagcacgcct ttggcggcga tttcggagtt tcttaagaat tgcagtgcca cgtacttgtc
    22861 gttttcgctg gcttctggat cgtgaagaat agccgcaacc tgtttctggt gtgccggacg
    22921 tagcatgaaa gaagcgtcgt gaaaggcttg ctgcgccagc agggtcaggg cttctggttc
    22981 cactttcagg atggtttcgc cgtcgaagtc ggcaacgcta acgtaatcgg aagtgagtag
    23041 atagtattcg gtattgtctt tccccatcgg gaaaggtgcc tggtagataa agggtttgtt
    23101 tgacatagct tccagcctgt aactctgtat ttgttattcg aaaatggcgt gccgcgtagt
    23161 cacgggcggc acgcaaagtg catttataag aacccgtaca tcgcggcgaa gatccagccg
    23221 aagacgcacg atacgctcac accaatcaac cccggcagaa taaaactgtg gttgatgacg
    23281 aagcgaccga tgtgggtggt gccggaacgg tcaaactgaa tcgctgccag atcgctcgga
    23341 taagtcggca ggatgtaata accgtagcaa gccggtgctg aagccacgat gtatgccgga
    23401 tcaacgccga tcgccagcgc gaccggaaca atcgccgcca gcgccgcagc ctgagagttt
    23461 acaaacttgg aaaccagcag cagaacaatg gcatacgccc acggatactc tttcaccatt
    23521 tcacccagta cgccctgaat ttcagacata tgcgcaccga acatggtttc tgccatccat
    23581 gcgataccgt acaccgccac gatggcgatc ataccggaac ggaagacttc gttttttgag
    23641 atagacgcgg gattggtttt ggtcaggata ataatcagcg ccccggtcag cagcataaac
    23701 atctgaataa ccagtaccat cgacagcggt ttgccgccga aggatggacg caggtccgaa
    23761 tcagcaccaa gaagggcgac tacagcgatt gccccgagga aaatccacat tgccagccag
    23821 ttgcttttcg gcagtttttt atccagcagc gtcgcggtat caccgtaaac atactcacgg
    23881 ttttccggta cggagatgaa tttctggaac tcttcgtctt tatccagatc tttaccgcgg
    23941 aaccagctga agataccgat cgccaggata ccgattaacg tcgatggaat ggtgattgcc
    24001 agcagatcga ggaactcaag atggcgacca tcaaaggtga cattacccag catcgcaacc
    24061 agagacacga ccgcaaccga caccggactg gcgataatcc ccatctgtgc accgatagaa
    24121 cttgccgcca tcggacgttc cggacggatg ttgttcttaa tggcgacgtc gtagatgatc
    24181 ggcagaatgg tgtaaaccac atgacccgta ccgcaaagaa tggtcagtgt acaggtcaca
    24241 aacggcgcga caattgagac atatttcggg ttgcggcgca gcagcttctc ggcaatttgc
    24301 agcatgacat caagaccgcc cgaagcttgc aaggtcgccg atgccgccac caccgcaatg
    24361 ataaccagca tgacatcaac tggtggttta cctggctgaa ggtggaagac gaagaccaga
    24421 atgaccagac cgataccgcc taataaaccc agcgcgatac caccctttct ggcaccataa
    24481 aacagacata ttagtattat gataagttgg atagtaaata acatgtgtga accctcgcga
    24541 taatcctatt taaatttttg ctgaatagat cacagtcacg ttctgttttg tatgaactgt
    24601 tttcagagcc tgactaaata tccgtttggt cgttaccggc tttagcaaat acctcacagt
    24661 gaatattggc tgattaaagc agcggcaaat ttatgcactg tgataagcgg ctttttcaga
    24721 gagaaaaact ctctggcgat gggtttatag tttgtactgc gcagatgtta atgttttgtt
    24781 tgctgttatc ttattgatat ttatcggtct attttttgtt ctcagggcag atatcattag
    24841 gtttatagat taatctgatc tacccatttg tgggtaaaaa tacacataat gcgggtgaca
    24901 taatagttaa ttaacttttg ttatcgtttt gaaattaaaa acaccgttca tctgaagaga
    24961 tattaatttt taacgatgat ggagggataa ttatatttga tctggcacaa gttttactga
    25021 ttaacgatgt aacttgtgcc aggggtattt tgcattacgg taataattat tacttacaga
    25081 taaccagcga tccgttattg gcaatattgt ttcagcagtg agtagtgttc tgcctgaata
    25141 cggtaacggt aaaccggacg ccccgtgacg ccataatgga tactggtgaa caagatgtgg
    25201 cagttgacca gccagatgag gtattttcgg caggaaacac gcgagatgtt aacctcgtta
    25261 gccagctcgt cggttgaaaa ctcatagtcc tgatgcgcgt caatccactg gcacagcgtg
    25321 cgtaacgtct gcggcgttaa gccttttggc aagcgacgag gatcctgttc gttggagctg
    25381 ctgccgtgga ttagctgatc aagctcggcc tggtcataat actgatgttt ttccagcgcc
    25441 attttctttt gccgccagcc ggtgagcgcc tcttcaaagc gggaagcctg gaagggtttg
    25501 atcaggtaat ccacgacacc gtaatgcagc gaatctttaa tggttgccgc atcggctgcg
    25561 gaggagatga caatcacatc acttttgcaa cgcgcgttat gcaggacagg cagtaaatcg
    25621 agcccgttct ctttttgcat atagatatcg agcaatatca ggtcgatagg cgcatcgcta
    25681 ttgaagataa tctctttggc tttctccagc gtcgaggctg ttccacagca ttgaaagcct
    25741 gggatttgtg ctacgtatcg gcgattcagc tccgcgacca ttgcgtcgtc atcgataatt
    25801 aatacattga tcatctgttc gacctctccc cgtcccaggg tatctggaca aaaaattgtg
    25861 tgaaaatccc cggttccgat tccacggcga tgctgccgcc gagattttct acctgttgtt
    25921 tgacaagtgc taaaccgacg cctcgctcgc ttccttttgt cgagacacct ttgtcaaaaa
    25981 tgtgatcgat tttgtcgggt gcgatccccg gtccatcatc gttaacttcg cagtgcagcc
    26041 agccgtgacg gtagtgcaat gttacgctaa tttcgccacc gggttccggc cctaatgctt
    26101 ccagcgcgtt ttctatcaga tttcccaatg tggtaatcag cgtcgcgacc tggtcctcac
    26161 tgccgctgtc tggcagctgg ctttcactgt ttaaaatcag cgtatggcct aaatcggtcg
    26221 cgcggttaat cttgctgatt aaaaaaccag cgataaccgg agatttgatc ttacccagca
    26281 gagagccaat ctcttcctga tagttattgg ctgttttgag aatgtaatct tccaactgct
    26341 tataactctt cagatgcaat aatccgagaa tcacatgcaa tttattcata aattcgtggg
    26401 atcgttcacg aagtgcgtca gcatagttga ccagaccgtc gagtcgctgc atcagtttac
    26461 gaacttcagt tttgtccctg aaggttgaaa tggcaccgat gataacgcca ttactgcgca
    26521 ccggaacggt gttgatcagt aatagccggt ctttaatcgt aatctcttcg tcgcggcgcg
    26581 gcgtaccgtc gcgtaacact tccgagacat ctaccacctg tgaccatgag tggctgagcg
    26641 tcgacagttt ctcatcgtcc tgcgacttac ggtaattcag caattcttgt gcggcatcgt
    26701 tgatcagcgt gacctcgccg cgatcgtcca cggcaacgac gccttctttg atagactgca
    26761 acatggcctg gcgttgctca aacagcgtgg agatttcgta gggttccagg ccgaaaagga
    26821 tttttttcag taccttaacc agaatgcagg tgccaatcag tccgaccagc atgccaaata
    26881 ataccgacca gataatgctc cagcgactgt cattgatctg ttgggtcacg cggcttaact
    26941 caaggccgat cgccaccacg ccaatttgtt tatgattttc atcgtagatg ggggtaaata
    27001 cgcgtaaagc ctgcgccaga aaaccgcgat tgatagcgac attttcttcg ccattcagcg
    27061 cattaaggat gtcatcacct ttaaatggct gaccaatacg ctgggcttca ggatgtgagt
    27121 agcgaagact ttgcatatcg gtaacgacaa taaacagcag atcgttgcgt ttgcgcacgg
    27181 cttccgcgat ggcctggatg ccactctcct gcggtttttt ctgcaagccc tgacggattt
    27241 ccggcgagtc ggcgagggta cgcgccactg ccagtgcctt gttggccagc ccgtctcgcg
    27301 tcatatcact gatttgcgag aagtaaatca gatgcaccac caatagcacc gagaacagta
    27361 ccgcgctgac cattaagatc acagtggtac tcaatttcat cggacgtttg cgtaatatgt
    27421 ggtagggcaa tgaatgtctc atcagcttcc ttgtgtgaca aatttcttaa gcattatctc
    27481 tgatgaggcg ggtaattcaa agggagtaaa aatgattggc tatatagggg aagagactct
    27541 ggcaacggaa actgccagtg ctgtatgaag attccggggc tatgcttata gcgataatca
    27601 tactgatgag agagggaagg tcatggatca ggcgctactg gacgggggtt atcgctgtta
    27661 taccggcgaa aagatcgatg tctatttcaa cactgcgata tgtcagcatt ctggcaattg
    27721 cgtacgtggc aacggcaagt tatttaatct caaacgaaag tcg
//
LOCUS       DAJTQQ010000052        27510 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000052 DAJTQQ010000000
VERSION     DAJTQQ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27510
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.052"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="OO309_002363"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="OO309_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR5847482.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="OO309_002364"
     CDS             complement(1614..1937)
                     /locus_tag="OO309_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR5847483.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO309_002365"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO309_002365"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR5847484.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO309_002366"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO309_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR5847485.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO309_002367"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO309_002367"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR5847486.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO309_002368"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO309_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR5847487.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="OO309_002369"
     CDS             7537..8574
                     /locus_tag="OO309_002369"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5847488.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVLDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO309_002370"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO309_002370"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR5847489.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="OO309_002371"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="OO309_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR5847490.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO309_002372"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO309_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR5847491.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="OO309_002373"
     CDS             10745..11626
                     /locus_tag="OO309_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5847492.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO309_002374"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO309_002374"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR5847493.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="OO309_002375"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="OO309_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR5847494.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO309_002376"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO309_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15728..15883
                     /gene="rseD"
                     /locus_tag="OO309_002377"
     CDS             15728..15883
                     /gene="rseD"
                     /locus_tag="OO309_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR5847495.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15880..16455
                     /gene="rpoE"
                     /locus_tag="OO309_002378"
     CDS             15880..16455
                     /gene="rpoE"
                     /locus_tag="OO309_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR5847496.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16488..17138
                     /gene="rseA"
                     /locus_tag="OO309_002379"
     CDS             16488..17138
                     /gene="rseA"
                     /locus_tag="OO309_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR5847497.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17138..18094
                     /gene="rseB"
                     /locus_tag="OO309_002380"
     CDS             17138..18094
                     /gene="rseB"
                     /locus_tag="OO309_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR5847498.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18091..18570
                     /gene="rseC"
                     /locus_tag="OO309_002381"
     CDS             18091..18570
                     /gene="rseC"
                     /locus_tag="OO309_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR5847499.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18768..20567
                     /gene="lepA"
                     /locus_tag="OO309_002382"
     CDS             18768..20567
                     /gene="lepA"
                     /locus_tag="OO309_002382"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR5847500.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20583..21557
                     /gene="lepB"
                     /locus_tag="OO309_002383"
     CDS             20583..21557
                     /gene="lepB"
                     /locus_tag="OO309_002383"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR5847501.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21829..22509
                     /gene="rnc"
                     /locus_tag="OO309_002384"
     CDS             21829..22509
                     /gene="rnc"
                     /locus_tag="OO309_002384"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR5847502.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22506..23411
                     /gene="era"
                     /locus_tag="OO309_002385"
     CDS             22506..23411
                     /gene="era"
                     /locus_tag="OO309_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR5847503.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23423..24151
                     /gene="recO"
                     /locus_tag="OO309_002386"
     CDS             23423..24151
                     /gene="recO"
                     /locus_tag="OO309_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR5847504.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24163..24894
                     /gene="pdxJ"
                     /locus_tag="OO309_002387"
     CDS             24163..24894
                     /gene="pdxJ"
                     /locus_tag="OO309_002387"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR5847505.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24894..25274
                     /locus_tag="OO309_002388"
     CDS             24894..25274
                     /locus_tag="OO309_002388"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR5847506.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25694..25774
                     /gene="shoB"
                     /locus_tag="OO309_002389"
     CDS             25694..25774
                     /gene="shoB"
                     /locus_tag="OO309_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR5847507.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25968..26228)
                     /gene="yfhL"
                     /locus_tag="OO309_002390"
     CDS             complement(25968..26228)
                     /gene="yfhL"
                     /locus_tag="OO309_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR5847508.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26443..27510
                     /locus_tag="OO309_002391"
                     /pseudo
     CDS             26443..27510
                     /locus_tag="OO309_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6780 a         6846 c         7215 g         6669 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 ctggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca tttttctttg
    15481 tttaatttac taaacattgt ttggtcggca tagcatcatg ttgtgcgaat aaacacctgc
    15541 tattttaata tttgttacag tggctaaaca cgttgacgca gggcggcgag aaaaagagaa
    15601 gttactggct ggtggaggat taggtggtga aataaaaagg ccgttgggtt actcttcagg
    15661 cagttaaatg ggcatttcta cacagataat gcgatgttca gattctgtag acttataatg
    15721 atagataatg atccgtctac agcatgacaa acaaaaacag atgcgttacg gaactttaca
    15781 aaaacgagac actctaaccc tttgcttgct caaattgcag ctaatggagt ggcgtttcga
    15841 tagcgcgtgg aaatttggtt tggggagact ttacctcgga tgagcgagca gttaacggac
    15901 caggtcctgg ttgaacgggt ccagaaggga gatcagaaag cctttaactt actggtagtg
    15961 cgctatcagc ataaagtggc gagtctggtt tcccgctatg tgccgtcggg tgatgttccc
    16021 gatgtggtac aagaagcttt tattaaagcc tatcgtgcgc tggattcgtt ccggggagat
    16081 agcgcttttt atacatggct gtatcggatt gctgtaaata cagcgaaaaa ttacctggtt
    16141 gctcaggggc gtcgtccacc ttccagtgat gtggatgcca ttgaagctga aaacttcgaa
    16201 agtggcggcg cgttgaaaga aatttcgaac cctgagaact taatgttgtc agaagaactg
    16261 agacagatag ttttccgaac tattgagtcc ctcccggaag atttgcgcat ggcaataacc
    16321 ttgcgggagc tggatggcct gagctatgaa gagatagccg ctatcatgga ttgtccggta
    16381 ggtacggtgc gttcacgtat cttccgagcg agggaagcta ttgataacaa agttcaaccg
    16441 cttatcaggc gttgacgata gcgggatact ggataagggt attaggcatg cagaaagaac
    16501 aactttccgc tttaatggat ggcgaaacgc tggatagtga gctgcttaac gaactggctc
    16561 ataacccaga aatgcagaaa acctgggaaa gctatcactt aatccgtgat tcaatgcggg
    16621 gtgatactcc cgaggtgctc catttcgata tctcttcacg cgtgatggcc gccattgaag
    16681 aagagccagt acgtcaatcg gcgacattga tcccggaagc ccagcctgcg ccgcatcaat
    16741 ggcagaaaat gccattctgg cagaaagtac gtccgtgggc ggcacagctt acccaaatgg
    16801 gcgtagccgc atgcgtatcg cttgcagtta tcgttggcgt ccagcactat aatggacaat
    16861 ctgaaacgtc ccagcagccc gaaacgccgg tatttaatac actgccgatg atgggtaaag
    16921 ccagcccggt aagcctggga gtaccttctg aagcgaccgc aaacaatggt caacagcagc
    16981 aggtacagga gcagcgtcgt cgcattaatg caatgttgca ggattacgaa ctgcaacgcc
    17041 gactccactc tgaacagctt cagtttgagc aggcgcaaac ccagcaagcc gctgtacagg
    17101 tgccaggaat tcaaacttta ggaacgcaat cgcagtaatg aagcaacttt ggtttgccat
    17161 gtcattagtg acaggtagcc tgttattctc tgctaacgcc tcggccactc ccgcgtccgg
    17221 ggcgttatta cagcagatga acctggccag tcagtcactg aattacgagc tgtcattcat
    17281 cagcatcaat aaacagggtg ttgagtctct gcgttatcga catgcacgcc tcgataaccg
    17341 tcctcttgca caattgttgc aaatggatgg cccgcgccgg gaagtggtac agcgcggcaa
    17401 tgaaatcagc tattttgaac cgggacttga accgttcacg cttaatggcg attacattgt
    17461 tgattctctg ccatcgctta tctataccga tttcaaacgc ctttctcctt actacgactt
    17521 tatctccgtc gggcgcacgc gtattgctga tcgtctttgc gaagtcattc gcgtggttgc
    17581 ccgagatggt acacgctaca gctacatcgt gtggatggac accgaatcga aattaccgat
    17641 gcgggttgat ctccttgatc gcgatggtga aacgctggaa caatttcgcg tgattgcttt
    17701 taacgtcaat caggatatca gcagcagtat gcagacgctg gcgaaggcaa atttgccgcc
    17761 gttgctttct gttcctgtag gtgaaaaagc taaattcagc tggacgccaa cctggttgcc
    17821 acagggtttt agcgaagttt ccagtagtcg acgtccgcta ccgacgatgg acaacatgcc
    17881 tatcgaatca cgtctctatt ccgacggatt attcagcttc tcggtaaacg ttaaccgcgc
    17941 tacgccatcg agcaccgatc agatgttgcg caccggacgc agaaccgtca gtacaagcgt
    18001 acgtgataac gccgaaatca ccattgtcgg tgaactgccg ccgcaaacgg cgaaacgcat
    18061 tgccgagaat attaagttcg gggcagcgca atgatcaaag agtgggctac cgtcgtctcc
    18121 tggcaaaacg ggcaggcgct ggtcagttgt gatgttaaag cttcatgcag cagctgcgct
    18181 tcacgcgccg gttgcggtag ccgcgtgttg aataaacttg gcccgcaaac cacgcatacc
    18241 attgtcgtac cctgtgatga gccgttagtg ccggggcaaa aagtggagtt ggggatcgcc
    18301 gaaggcagcc tgcttagctc cgcattactg gtttatatgt cgccgctggt gggattattc
    18361 ctcatcgctt cgctatttca gctactcttt gcttctgacg tcgcagcatt atgtggtgcg
    18421 gtcctcggcg gtattggcgg gttcctgatt gcgcgcggct actcgcgtaa gtttgctgcc
    18481 cgggcagaat ggcaaccgat catcttaagc gtggcattgc cgcctggcct tgtgcgattt
    18541 gaaacatctt cggaagacgc gagccagtga tttcactggc tttgttgcat tttgcataac
    18601 aaatgcgagg acgtttccag ataccacaat ccatacggta tgaacatttc ctcgcctcaa
    18661 tgttgtagtg tagaatgcgg cgtttctatt aatacagacg ttaagctcag aacagcgact
    18721 tctaaaagcc tggttaaccg ggcattaagg cacaataatc atactttatg aagaatatac
    18781 gtaacttttc gatcatagct cacattgacc acggtaaatc gacgctgtct gaccgtatta
    18841 tccagatctg cggtggcctg tctgaccgtg aaatggaggc gcaggttctc gattccatgg
    18901 atcttgagcg tgagcgtggc attaccatca aagcgcaaag cgtgacgctg gactacaaag
    18961 cgtctgacgg cgaaacctat cagcttaact ttatcgacac cccgggccac gtagacttct
    19021 cctatgaagt ttcccgttcg ctggctgcct gtgaaggtgc attgctggtg gtcgacgccg
    19081 ggcagggcgt agaagcgcaa accctggcaa actgctacac cgccatggaa atggatctcg
    19141 aagtggtgcc ggtactgaac aagattgacc tgccggcagc cgatcctgaa cgcgtggcgg
    19201 aagaaattga agatatcgtc ggcatcgacg ccaccgatgc agtgcgctgt tcagcgaaaa
    19261 ccggcgttgg cgtgcaggac gttctcgaac gtctggtgcg cgacattccg ccgccggaag
    19321 gcgatccgga aggcccgttg caggcactaa ttatcgactc ctggttcgac aactacctgg
    19381 gcgttgtttc acttatccgt attaaaaacg gcaccctgcg taagggcgac aaagtgaaag
    19441 tcatgagtac cgggcagacc tataacgccg accgtctggg catcttcacg ccgaaacagg
    19501 ttgaccgcac tgaactgaaa tgtggcgaag taggctggct cgtatgtgcg attaaagata
    19561 tccacggcgc tccagtcggc gataccttaa cgctggcgcg taatccggca gaaaaggcgc
    19621 tgcctggctt taagaaagtc aaaccgcagg tatacgccgg tctgttcccg gtaagttccg
    19681 acgactatga agccttccgt gacgcgctgg gtaaactcag cctgaacgat gcctcactgt
    19741 tctatgagcc ggaaagctcc agcgcgctgg gctttggttt ccgctgcggc ttccttggcc
    19801 tgctgcacat ggagatcatc caggagcgtc tggaacgtga atacgatctg gatctgatca
    19861 ccactgcgcc gaccgtagtg tatgaagttg aaaccacgtc aagggaagtt atctacgtcg
    19921 acagcccatc caagctgcct gcggtaaata acatttacga actgcgcgag ccgattgcag
    19981 agtgtcacat gctgctgccg caggcatatc tcggcaacgt tattacgctg tgcgtagaaa
    20041 aacgcggcgt gcagaccaat atggtttacc acggtaatca ggtggcgctg acgtacgaga
    20101 tcccgatggc ggaagtggtg ctcgacttct tcgatcgcct gaaatctacc tcgcgtggtt
    20161 atgcgtctct ggattacaac ttcaaacgct tccaggcgtc cgacatggta cgtgtagacg
    20221 tattaatcaa cggtgaacgt gttgatgcgc tggcgttgat cacccaccgt gataattcgc
    20281 aaaaccgcgg tcgcgagttg gtggagaaga tgaaagatct gatcccacgc cagcagtttg
    20341 atatcgccat tcaggcagcg attggtacgc acattattgc gcgatccacc gtgaaacagc
    20401 tgcgtaaaaa cgtactggct aaatgttatg gcggcgatat cagccgtaag aaaaagctgc
    20461 tgcagaagca gaaagaaggt aagaaacgca tgaagcagat cggtaacgtt gagctgccgc
    20521 aggaagcgtt cctcgccatt ctgcacgtcg gcaaagacaa caaataaccc ttaggagttg
    20581 gcatggcgaa tatgtttgcc ctgattctgg tgattgccac actggtgacg ggcattttat
    20641 ggtgcgtgga taaattcttt ttcgcaccta aacggcggga acgtcaggca gcggcgcagg
    20701 cggctgccgg tgactcactg gataaagcaa cgttgaaaaa ggttgcaccg aagcctggct
    20761 ggctggaaac cggagcttct gtttttccgg tgctggctat cgtattgatt gtacgttcgt
    20821 ttatttatga accgttccag atcccgtcag gttcgatgat gccgactctg ttaatcggtg
    20881 attttattct ggtagagaag tttgcttatg gcattaaaga tcctatctac cagaaaacgc
    20941 tgatcgaaac cggtcatccg aaacgcggcg atatcgtggt ctttaaatat ccggaagatc
    21001 caaagcttga ttacatcaag cgcgcggtgg gtttaccggg cgataaagtc acttacgatc
    21061 cggtctcaaa agagctgacg attcaaccgg gatgcagttc cggccaggcg tgtgaaaacg
    21121 cgctgccggt cacctactca aacgtggaac cgagcgattt cgttcagacc ttctcacgcc
    21181 gtaatggtgg ggaagcgacc agcggattct ttgaagtgcc gaaaaacgaa accaaagaaa
    21241 atggaattcg tctttccgag cgtaaagaga cactgggtga tgtgacgcac cgaattctga
    21301 cagtgccgat tgcgcaggac caggtgggga tgtattacca gcagccaggg caacaactgg
    21361 caacctggat tgttccgccg ggacaatact tcatgatggg cgacaaccgc gacaacagcg
    21421 cggacagccg ttactggggc tttgtgcctg aagcgaatct ggtcggtcgg gccacggcta
    21481 tctggatgag cttcgataag caagaaggcg aatggccgac tggtgtgcgc ttaagtcgca
    21541 ttggcggcat ccattaatag ccatcttcgt tcacgttgtc gccgttatgg cgacaacgtg
    21601 aattatttat gagataaatc tcccgtggct aacgacatcc cccgtcgttg tgtatagaat
    21661 attcccccga agtttaaggt tggcaccttc aggttgccac ggcacacgaa acagcgttgg
    21721 tccccatata ccgctaaact gaaactgcag cgaagcagtt agcagagcca tgtatatcag
    21781 gtctgtttcg tgtgctgaat tgttgacgca tttatttatt ggtatcgcat gaaccccatc
    21841 gtaattaatc ggcttcaacg gaagctgggc tacactttta atcatcagga actgttgcag
    21901 caggcattaa ctcatcgtag tgccagcagc aaacataacg agcgtttaga atttttaggc
    21961 gactctattc tgagctacgt tatcgccaat gcgctttatc accgtttccc tcgtgtggat
    22021 gaaggcgata tgagccggat gcgcgccacg ctggtccgtg gcaatacgct ggcggaactg
    22081 gcgcgcgaat ttgagttagg cgagtgctta cgtttagggc caggtgaact taaaagcggt
    22141 ggatttcgtc gtgagtcaat tctcgccgac accgtcgaag cattaattgg tggcgtattc
    22201 ctcgacagtg atattcaaac cgtcgagaaa ttaatcctta actggtatca aactcgtctg
    22261 gacgaaatta gcccaggcga taaacaaaaa gatccgaaaa cgcgcttgca agaatatttg
    22321 cagggtcgcc atctgccgct gccgacttat ctggtagtcc aggtacgtgg cgaagcgcac
    22381 gatcaggaat ttactatcca ctgccaggtc agcggcctga gtgaaccggt ggttggcaca
    22441 ggttcaagcc gtcgtaaggc tgagcaggct gccgccgaac aggcgttgaa aaaactggag
    22501 ctggaatgag catcgataaa agttactgcg gatttattgc catcgtcgga cgtccgaacg
    22561 ttggcaaatc cacattgttg aacaaactgc tggggcagaa aatctccatc acctcccgca
    22621 aggcgcagac aactcgtcac cgcattgtgg ggatccatac tgaaggcgcg tatcaggcga
    22681 tctacgtcga tacaccgggc ctgcatatgg aagaaaaacg cgccattaac cgcctgatga
    22741 acaaagcggc gagcagctct attggcgatg ttgagctggt gatttttgtc gttgaaggca
    22801 cccgctggac gccggacgac gaaatggtgc tcaacaaact gcgcgaaggc aaagcgccgg
    22861 taatcctcgc ggtgaacaaa gtggacaacg tgcaggagaa agccgatctg ctgccgcacc
    22921 tgcagttcct ggcaagccag atgaacttcc tcgatatcgt gccaatctct gccgaaaccg
    22981 ggctgaatgt tgacactatt gcggcaatcg tgcgtaagca tctgcctgaa gcgactcatc
    23041 acttcccgga agattacatc accgatcgct cacagcgttt tatggcgtct gaaatcatcc
    23101 gcgaaaaact gatgcgtttc cttggcgctg aactgccgta ctccgtgacc gtggagatcg
    23161 aacgtttcgt ctctaacgaa cgcggcggtt gtgacatcaa cggtttgatt ctcgttgagc
    23221 gtgaagggca gaagaagatg gttattggca acaaaggggc caagatcaaa accatcggga
    23281 ttgaagcgcg taaagacatg caggaaatgt tcgaagcgcc tgtccacctt gaactgtggg
    23341 taaaagtgaa atcgggttgg gccgacgacg aacgcgcact gcgcagtctc ggttatgttg
    23401 acgatcttta agagtcactc cgatggaagg ctggcagcgc gcatttgtcc tgcatagtcg
    23461 cccgtggagc gaaaccagcc tgatgctgga cgtcttcaca gaggaatcgg gtcgcgtgcg
    23521 tctggttgcc aaaggcgcac gctctaaacg ctctactctg aaaggtgcat tacagccttt
    23581 cacccctctc ttgctacgtt ttggcgggcg tggcgaagtc aaaacgctgc gcagtgctga
    23641 agctgtctca ctggcgctgc cattaagcgg tatcacgctt tacagcggtc tgtacatcaa
    23701 cgaacttctc tcccgcgtac tggaatacga gacgcgcttc tctgaactct ttttcgatta
    23761 cttgcactgc attcagtctc ttgcaggtga cactggtacg ccagaacccg cgctgcgtcg
    23821 ctttgaactg gcactactcg ggcatctggg ctatggcgtc aattttaccc attgcgcggg
    23881 tagcggcgag ccggtagatg gcaccatgac gtatcgttat cgcgaagaaa aagggtttat
    23941 cgcaagcgtc gttatcgaca ataaaacgtt caccggaagg cagttaaaag cgttaaacgc
    24001 gcgggaattt cctgacgcag acacactacg cgccgcgaaa cgctttaccc gaatggcgct
    24061 taagccgtat cttggcggta aaccattaaa gagcagggaa ctgttccggc agtttatgcc
    24121 taagcgaacg gtgaaaacac attatgaatg atgaggattg tcatggctga attactgtta
    24181 ggcgtcaaca ttgaccatat tgcaacgctg cgtaacgcac gcggtactgc ttacccggat
    24241 ccggtgcagg cggcgtttat tgccgagcag gcgggagcgg acggcattac cgtgcattta
    24301 cgtgaagatc gccgccacat tactgaccgc gatgtgcgca tcctgcggca gacgctggat
    24361 acccgaatga atctggagat ggcagtgacc gaagagatgc tggcgatcgc cgttgagacg
    24421 aagccacatt tttgctgctt ggtaccggaa aagcgtcagg aagtaacaac cgaaggcggc
    24481 ctggacgttg cagggcagcg tgaaaaaata cgcgatgcct gcaaacgtct ggcagatgcc
    24541 gggattcagg tttctctgtt tatcgacgcc gatgaagagc agatcaaagc tgcggcagag
    24601 gttggcgcac cgtttatcga gatccacacc ggttgctatg ctgatgccaa aactgacgcc
    24661 gaacaggcgc aagagctggt gcgtatcgcc aaagccgcga cctttgccgc tagtctcggt
    24721 ctgaaagtta acgccggaca cggtctgacc tatcacaacg tgaaagccat tgccgccatc
    24781 cctgagatgc atgaactgaa tatcggtcat gccattattg gccgtgcagt gatgaccgga
    24841 ctgaaagatg cggtggcaga aatgaagcgt ctgatgctgg aagcgcgtgg ctaatggcaa
    24901 tattaggttt aggcacggat atcgtagaga tcgctcgcat agaagcagtg atcgcccgat
    24961 ccggtgagcg cctggcacgc cgcgtattaa gcgataacga atgggcaatc tggaaaacgc
    25021 accaccagcc ggtgcgtttt ctggcgaagc gttttgctgt gaaagaagcc gcagcaaaag
    25081 cgtttggcac cgggatccgc aatggtctgg cgtttaatca atttgaagta ttcaatgatg
    25141 agctcggcaa accacggcta cggctatggg gtgaggcatt aaaactggcg gaaaagctcg
    25201 gcgttgtaaa tatgcatgta acgctggcag atgaacgcca ctatgcttgt gccacggtaa
    25261 ttattgaaag ttaaaataat accgttctct ttaaaaagta aaacccccga actggtaagg
    25321 aacgggggtt ttacttttta actttaattt tgtgcagcat gcaccctcaa caacagcgat
    25381 tatggatcag tgtacttatc ttttacaagc aacttgcaat ctttagcata aaaactcgag
    25441 cctttacgaa gaaagcaata ttgatggaaa gattaacgtg accgccaatt cgtaagtaca
    25501 ttaaaattgg cttcgttatt gaagattttg ctgtgcttta caccatgcca cagaattccc
    25561 ccattgaaac gagtggtgtc gtcaaagctc tggtgtggag tgcagcatgc accctcaata
    25621 actcgcacgt tcagttttgg ggagatgtaa gggctaatct gaatggctgc attccttgtt
    25681 taaggaaaaa cgaatgactg attgccgata cctgattaaa cgggtcatca aaatcatcat
    25741 tgctgtttta cagctgatcc ttctgttctt ataacacaag gaaacgtact taaggtgcgt
    25801 ccggtgaacc aatcggacgc gcctttaata actataaata agtgtcttgg cagatactat
    25861 ataaattaac ttagtgaatg attatgctaa tgtcatcaat taaataaata taatggcgtt
    25921 aaggcttccc agtaatataa ttaatactct acttccagag tagaatatta aattttatcc
    25981 gcgtggtgca tcagcacaaa tttatcccac aactgttctt ctgtctcgac atgcgccgga
    26041 tctttcacaa tagtattggg gatcgggcac accttctggc aggttggtgt ctcgtagtgc
    26101 cctacgcatt cggtacactt atcgctgtta atttcgtaga tatgatctcc cattgaaatc
    26161 gcctcattcg ggcattcggg ttcacacata tcacaattaa tacagcgttt agtaattagt
    26221 aaagacattt caatggatta ccgttaaatc attttaaaat cagtaagttg tatcgagttt
    26281 gtatgcttta ctgtcattaa cttactgtat gttgatccag tgtatttaac cttgataaac
    26341 tcagtccagc aacacaaaac cgcaacacat tgcattttgt cccgtagaaa agacttgtat
    26401 gtgtgagctt gttttctgcg cctacgcaga taaggattga gaatgccgcg cactgtaaca
    26461 cataatccgg atagccccaa taatgacgat gttttagccg catctgaaaa atgggacgcc
    26521 tgtaaacccc cctataccag cgcacacatg aaaatctgtg ttgctgccgc caaaatcatc
    26581 ctcgctgctt ccggcgtggc tcgctgttcc aaatacgaaa aagagaacta tctccgtatc
    26641 gatttcagca aagccggtaa ggttacattt tacgccgagt ttccaaaaaa gatgggcctc
    26701 aaaggtaaaa agctcggcga gtggccggag ctcgttatcc agctggcgcg cgaaaaagca
    26761 ctaggtatgg ctgacggtgg actgcgggca gagtccgtac atgcagcgct ggaaatgtac
    26821 cgggatgacc caaagccaaa gtagcccggc agaagctgag cccggacagt ttcacaacct
    26881 acggggtgcg tatcgaccgg attaaagtaa cgttcggcga gcgtgaggtg ttcagcgacg
    26941 taacatacaa tcggctggtg gaagtgctgg acgagtggat cgccactcgc tcgaacaata
    27001 acgccctgga gttgtttgcc gagctccgtc ggttctggaa gttctgcgca cctactcttt
    27061 gcaacggccg caatgttgcc gccagtctgc cagatgatta tgtttcctcc cgcgtacaga
    27121 aacctacccc cacacggctt tttaccgata ttgaatcaat cgcccgactc tggctcaatg
    27181 ttgctgcctg cacctctgta caccagaaga atgctgttcg cttcatgatc atcactggtg
    27241 ttcgtccgat taatgtccat aacctgcgct gggactacgt ttacgaggag gctggtgaaa
    27301 ttgtttatcc ggaaggggtt atcggcatgc gaggggctat gaaaacacaa aaggctttcc
    27361 gcctgccgat aacgcctgag atccggcgga ttatcgacga gcagaaagcc tggcgtgatt
    27421 cagttcctga gtgcaacagg gattatgtat ttttgcagcc acgtgatcca atgcagccat
    27481 tttcaaaacg atcactggat aagctggtga
//
LOCUS       DAJTQQ010000053        26315 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000053 DAJTQQ010000000
VERSION     DAJTQQ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26315
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.053"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..171
                     /locus_tag="OO309_002392"
     CDS             <1..171
                     /locus_tag="OO309_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HCR5847509.1"
                     /translation="FFHSLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGG
                     LSPEQFENQNLA"
     gene            complement(168..308)
                     /locus_tag="OO309_002393"
     CDS             complement(168..308)
                     /locus_tag="OO309_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCR5847510.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSDPTHVIWT
                     QA"
     gene            550..843
                     /locus_tag="OO309_002394"
     CDS             550..843
                     /locus_tag="OO309_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR5847511.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            915..1574
                     /locus_tag="OO309_002395"
     CDS             915..1574
                     /locus_tag="OO309_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR5847512.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1666..2112
                     /locus_tag="OO309_002396"
     CDS             1666..2112
                     /locus_tag="OO309_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR5847513.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2319..3219)
                     /gene="hlyE"
                     /locus_tag="OO309_002397"
                     /pseudo
     CDS             complement(2319..3219)
                     /gene="hlyE"
                     /locus_tag="OO309_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3592..4011
                     /locus_tag="OO309_002398"
     CDS             3592..4011
                     /locus_tag="OO309_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR5847514.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            4011..5279
                     /gene="umuC"
                     /locus_tag="OO309_002399"
     CDS             4011..5279
                     /gene="umuC"
                     /locus_tag="OO309_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR5847515.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5325..5855)
                     /gene="dsbB"
                     /locus_tag="OO309_002400"
     CDS             complement(5325..5855)
                     /gene="dsbB"
                     /locus_tag="OO309_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR5847516.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(6001..7542)
                     /gene="nhaB"
                     /locus_tag="OO309_002401"
     CDS             complement(6001..7542)
                     /gene="nhaB"
                     /locus_tag="OO309_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR5847517.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7763..8482
                     /gene="fadR"
                     /locus_tag="OO309_002402"
     CDS             7763..8482
                     /gene="fadR"
                     /locus_tag="OO309_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR5847518.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8534..10066)
                     /locus_tag="OO309_002403"
     CDS             complement(8534..10066)
                     /locus_tag="OO309_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR5847519.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10396..11694
                     /gene="dadA"
                     /locus_tag="OO309_002404"
     CDS             10396..11694
                     /gene="dadA"
                     /locus_tag="OO309_002404"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR5847520.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11704..12774
                     /gene="dadX"
                     /locus_tag="OO309_002405"
     CDS             11704..12774
                     /gene="dadX"
                     /locus_tag="OO309_002405"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR5847521.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12942..14356)
                     /locus_tag="OO309_002406"
                     /pseudo
     CDS             complement(12942..14356)
                     /locus_tag="OO309_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14967..16703)
                     /locus_tag="OO309_002407"
     CDS             complement(14967..16703)
                     /locus_tag="OO309_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR5847522.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16798..17712)
                     /gene="ldcA"
                     /locus_tag="OO309_002408"
     CDS             complement(16798..17712)
                     /gene="ldcA"
                     /locus_tag="OO309_002408"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR5847523.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17812..18423
                     /gene="emtA"
                     /locus_tag="OO309_002409"
     CDS             17812..18423
                     /gene="emtA"
                     /locus_tag="OO309_002409"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR5847524.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18425..19159)
                     /locus_tag="OO309_002410"
     CDS             complement(18425..19159)
                     /locus_tag="OO309_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR5847525.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19360..19614
                     /locus_tag="OO309_002411"
     CDS             19360..19614
                     /locus_tag="OO309_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR5847526.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19664..21124)
                     /locus_tag="OO309_002412"
     CDS             complement(19664..21124)
                     /locus_tag="OO309_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR5847527.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(21061..21618)
                     /locus_tag="OO309_002413"
     CDS             complement(21061..21618)
                     /locus_tag="OO309_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCR5847528.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21644..22498)
                     /locus_tag="OO309_002414"
     CDS             complement(21644..22498)
                     /locus_tag="OO309_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCR5847529.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22498..23307)
                     /locus_tag="OO309_002415"
     CDS             complement(22498..23307)
                     /locus_tag="OO309_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR5847530.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23317..24075)
                     /locus_tag="OO309_002416"
     CDS             complement(23317..24075)
                     /locus_tag="OO309_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5847531.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(24072..25052)
                     /locus_tag="OO309_002417"
     CDS             complement(24072..25052)
                     /locus_tag="OO309_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR5847532.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(25052..26074)
                     /locus_tag="OO309_002418"
     CDS             complement(25052..26074)
                     /locus_tag="OO309_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCR5847533.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
BASE COUNT         6581 a         6445 c         6812 g         6477 t
ORIGIN      
        1 ttctttcatt cgctgaaagt ggaatgtatc catggagaac actttatcag ccgggaaata
       61 atgcgggcaa cggtgtttaa ttatatcgaa tgtgattaca atcggtggcg gcggcacagt
      121 tggtgtggcg gcctcagtcc ggaacaattt gaaaaccaga acctcgctta ggcctgtgtc
      181 catattacgt gggtaggatc actatggcct ttgctgaact caacattgac attcttaccc
      241 gcagcaaagc tcgtggaggt tagcagtgca agcagcaata cagccttact gatgctcttg
      301 attttcattg gtagtctcct gttcagaatg agtacgatga actgttataa tataacaatc
      361 ccttacggga gaattccagt ggcttttctg cgttcggtaa aatcccggcg cggcattttt
      421 tgccgggggg attacttata aaggggcatg gactcaacaa accgcagaaa tctacagctg
      481 gctggcttgt ggcgcgggtt tcattgatgt tagcttatgc caaaacctgg catcttaaag
      541 agtaaaagta tgttttgtgc gatttatcga agcagcaagc gtgaccagac ctatttatat
      601 gtcgaaaaaa aagacgattt ttcgcgtgtt cctgaggaac tgatgaaagg ttttggtcag
      661 cctcagttag cgatgattct gccgttggat gggcggaaga aactggtcaa tgctgatatt
      721 gaaaaggtta aactggcatt aaccgagcaa ggttactatt tgcagttacc gccaccaccc
      781 gaagatttgc tgaagcaaca tctttccgtc atggggcaga aaacagacga cactaacaaa
      841 taaccgatat ccggcggtgg cattatcttt gtcggcgcgg gttttcatat ccacgataag
      901 gtgaggggaa cgttatgtat caacatcaca actggcaagg tgcgctgctg gattatccgg
      961 tgagtaaagt agtctgtgtt ggcagtaact atgccaaaca tattaaagag atgggcagcg
     1021 cagtgcccga agagccagtg ctgtttatta aaccagaaac ggcactgtgc gatctgcggc
     1081 agccattggc gatcccatcc gatttcggtt cagttcatca tgaagtcgaa ctggcggtgt
     1141 tgattggcgc gacgctgcgt caggcaacgg aagagcatgt ccgcaaagcc attgccggtt
     1201 acggcgtggc gcttgatctg acattgcgtg atgttcaggg aaaaatgaag aaagccgggc
     1261 agccgtggga aaaggctaaa gcgtttgata actcttgtcc gctttccggg tttatccccg
     1321 cggcggaatt taccggcgat ccacaaaata caacgctggg cctgagcgta aacggcgaaa
     1381 aacgccagca aggtacgact gcggacatga tccataaaat cgttccgttg atcgcttata
     1441 tgagcaagtt ttttaccctc aaggccggtg acgttgtgtt gacaggcacg cctgatggcg
     1501 tcggcccgtt gcaaagcggt gatgagctga cagtcacttt cgatgggcat tctttgacaa
     1561 ctcgcgtttt gtaatacttt ttgccgccag aaagcggcgg caaaacttgc atcgctgtgc
     1621 cagactggtt ataaggtgcg ttttaacgta atggcggaac acctgatgag cgatgtacct
     1681 ttctggcaaa gtaaaaccct ggacgaaatg agcgatgcgg aatgggagtc gttgtgtgat
     1741 ggttgcggtc agtgttgctt gcataaactg atggatgaag acaccgacga aatctacttc
     1801 actaacgtcg cctgtcgcca gctcaatatt aaaacctgtc aatgtcggaa ctacgaacgt
     1861 cgtttcgagt ttgaacccga ctgcattaaa ttaacccgtg aaaatctgcc aacattcgaa
     1921 tggctgccaa tgacctgcgc ttatcggttg ctggcggaag gtaaagattt acctgcgtgg
     1981 catccgctac ttactggttc gaaagcggca atgcatggtg aacgtatctc tgtgcgtcat
     2041 atcgcagtga aagaatcaga agtgattgac tggcaggatc atatcttaaa taaacctgac
     2101 tgggcgcagt gataaattta atagatgctg atgggggctt tcttaatata cgtctggatg
     2161 ctcgccattt cgtagccctg tttttgaaag cgctctgtca taatgagagt tacccgatac
     2221 cttattatca acaggtaact ctcaatcttt aaaaaacgtc tgtgttttta aatgtgataa
     2281 accttatacc ttgagtacat ggagagaata atcgcttatc agacttcagg tacttcaaag
     2341 agtgtctttt taccgtgtct tttctgatac tcattacagg tgttaatcat ttttttggcc
     2401 gctgctttta gcaaagaaag cattaaatca tcataatcaa catagaatct ggttgtttca
     2461 gtttccgttt ttatctcccc gatggcggct atttcggtgg ttaatttcaa tttggcggca
     2521 tcgatatctt tattcgcttg tttaaccgtg ttagacaggg tggtaaagaa actctgcaca
     2581 gattttaact tgttcttcaa ttctggaatc agtttccctt caactacgcc cgcagcaata
     2641 gaataggaaa tgattaaacc aaatggaccg gcgacgacat cggctgcggc accggcatac
     2701 gcttccttcc tgattttatc tacctgtgac tggaaatagc tgcttttttc tgaaaaatca
     2761 ttggttaact ggctatctaa cgccagcagt ttcccggaag cgttgttgaa actttgtgag
     2821 cttaccagca gggatttttg cgcttcattc agcttcgtga tgccgtcatc cagtacctta
     2881 atgaggggcg gatgctttct tctcattgta ctcatcaaat agcaaaatat acgctgcgag
     2941 caattgcgtc gcaacaccac accattcata cactgtttgg gttgcttcaa aatacttatc
     3001 ctggctatcc ataagtaagg ttttaatatc gccgactaaa acggaggctg cctgtgaata
     3061 ctcctgttta aagcgactta actcttttat ggtttcatca aaggtctgcc aggggatgac
     3121 ctgatcgaga tatttattat aaagatctaa tgctccatct gcggtttcga ttgcgttttt
     3181 aactacttct accgttttat ctgcaacgat ttcagtcata atcattcgcc tctttaaata
     3241 tataaattgt aatgaaactc ctgttttaca actattaata aattttactt catctaattc
     3301 atagttagcc gggcgggatg cgtcaatgtc tttatttcta ttaatatgat aaatatcaaa
     3361 caatgtttaa tgtcattatg gcgaatgctt ctattctatt tttttgccgg gtgatatttt
     3421 tcatttctgc tggatgagcg tcgtcgccag aaggccacgt gagcacagga taagagaacg
     3481 aaaaatcagc agtctatgca gcgacaaata ttgatagcct gaatcagtat tgatctgctg
     3541 gcaagaacag accactgtat ataaaaacag tataacttca ggcagattat tatgttgttt
     3601 atcaagcctg cggatctccg cgaaattgtg acttttccgc tatttagcga tcttgttcag
     3661 tgtggctttc cttcaccggc agcagattac gttgaacagc gcatcgatct gaatcaactg
     3721 ttgatccagc atcccagcgc gacttacttc gtcaaagcaa gtggtgattc tatgattgat
     3781 ggtggaatta gtgacggtga tttactgatt gtcgatagtg ctattaccgc cagccatggt
     3841 gatattgtca tcgctgctgt tgacggcgag tttacggtga aaaaattgca actacgcccg
     3901 acggtacagc ttattcccat gaacagtgcg tactcgccca ttaccatcag tagcgaagat
     3961 acgctggatg tctttggtgt ggtgatccac gtcgttaagg cgatgcgctg atgtttgccc
     4021 tctgtgatgt aaacgcgttt tatgccagct gtgagacggt gtttcgccct gatttatggg
     4081 gtaaaccggt ggttgtgcta tcgaataatg acggttgcgt tatcgcccga aacgctgagg
     4141 caaaggcgct gggcgttaaa atgggcgatc cctggttcaa acaaaaagat ctgtttcgtc
     4201 gctgtggtgt ggtttgcttt agcagcaatt atgagcttta cgcagacatg agcaatcggg
     4261 tgatgtcgac gctggaagaa ctatcgcccc gcgtcgagat ttacagtatt gatgaagcat
     4321 tctgcgatct gacaggtgtg cgtaattgtc gcgatctgac tgattttggc agagaaattc
     4381 gcgcaacggt gctacaacgt acccatctta ctgttggtgt ggggatcgcc cagaccaaaa
     4441 cgttggctaa gcttgccaat catgcggcaa aaaaatggca gcggcagacg ggtggggtgg
     4501 tggatttatc aaatctggaa cgccagcgta aattaatgtc tgctctcccc gtggatgacg
     4561 tctgggggat tggacggcgg atcagcaaaa aactggacgc gatggggatc aaaaccgttc
     4621 tcgatttggc ggatacagat atccggttta tccgtaaaca ttttaatgtc atgctcgaaa
     4681 gaacggtgcg tgaactgcgc ggcgaaccct gtttgcaact ggaagagttt gcaccgacga
     4741 agcaggaaat tatctgttcc cgctcgtttg gtgaacgcat cacggattat acgtcgatgc
     4801 ggcaggccat ttgtagttac gctgcccggg cggcggaaaa acttcgcagt gagcatcaat
     4861 attgtcggtt tatctccaca tttattaaga cgtcaccatt tgcgctcaat gaaccttatt
     4921 acggcaatag cgcgtcggta aaactgctga cgcccactca ggacagcagg gatatcatta
     4981 acgctgctac gcgatctctg gatgccatct ggcaagcggg ccatcgctac caaaaagcgg
     5041 gcgtgatgct gggggatttc ttcagtcagg gagtcgcgca gctcaattta ttcgatgaca
     5101 acgcaccgcg ccccgggagt gagcaattga tggcggtaat ggatactctg aatgctaaag
     5161 agggcagagg aacactctat tttgccgggc aggggatcca acaacaatgg cagatgaagc
     5221 gagctatgct ttcaccacgt tatacaacgc gaagttctga tttactgcgg gtcaaataaa
     5281 tatagcggca ggaaaaaagc gctcccgcag gagcgccgaa tggattagcg accgaacaga
     5341 tcacgttttt tcgctttaaa cggctgggaa atcaccacca gcaccgcgac aatcaggtaa
     5401 gcgataaaaa taccgagcag ccactgcggc atttccatac ctaaaaaatc ccactgacgc
     5461 tcggcgcaat cgccagaggc gacaaacact tgcggcaccc atttatccag cggcagccat
     5521 tccgggaaac gaaccataaa atcacaggtg gcaaatggcg aaggatagag ctgaagcatg
     5581 gtgtgctcgt aagttaactg cacaccgcgg aacgcactat acaaccagat aaccatcgct
     5641 acataacgca gcggagtttt cggggcgatc gcgccaatca gcgcagcacc cagaacgccg
     5701 aataacgcgc agcgttcata aatacagagc acgcaaggtt tcagtaacat cacatgctgg
     5761 aaccacagcg ccgtcagttc cagtgccaga gcagtaaacg ccatcaacag ccacgcgccc
     5821 cggccttgtg aacattggtt caaaaatcgc aacataatca tttccctgca atatgcatag
     5881 agtgcgcagt ttaaaccaat tcattcgctg cgccaccagg aggcagcaaa aataggacgc
     5941 aattggacaa ttatcgggcg aaaaggcaaa ccgggcattt atatgcccgg taagttgtta
     6001 tcaaagcgtt gctatccagc ccatttgcat aaaccattcg gttacagggg caagcgtaaa
     6061 ctcgacgcag agcaagccga cgagtgtcag tacgagggtg taaggcaggg ccatccacac
     6121 catgcggcca taagagaggc gaatcaatgg cgcgagtgca gaggtcagca ggaacaggaa
     6181 cgcagcctga ccgttcggcg tagcgacaga gggcagattg gtaccggtat taatggcgac
     6241 cgccagcagc tcgtattgct tcaacgtgat agcgccactt tccattgccg ctttcgcttc
     6301 gttgatataa atcgtcccca cgaagacgtt atccgaaatg gatgacagca gaccgttgaa
     6361 aatatagaac agcgacagct gagcatgttc agatgcctgc aacacaaact gaataatcgg
     6421 cgaaaacagt tgttggtcga taatcaccgc gactaccgag aaaaacaccg tcaacagtgc
     6481 ggtgaatggc agagattcgg tgaaggcttt accgatagca tgttcatcgg tgacaccggt
     6541 caatgatgtt gccagaataa tgactgacaa accaatcaag ccaacttccg ccagatgcaa
     6601 cgccagcgca gtcaccagcc agacgccaat aatcgcctgg acaatcagac ggattttatc
     6661 ctgacgggta cgctggtggc ggctttgatc gtcaaactgt tgcaacactt cgcggacttt
     6721 ctccggcagc gtttcaccgt agccaaacca acgcagcttc tctaccagca ggcaggttaa
     6781 caggccacaa atcagaaccg gaacggtcac cggcgacatg cgcaggaaga aatcgccaaa
     6841 atgccagcca gccgctttag cgatgatcag gttctgtggt tcgcccacca tggtcattac
     6901 gccgcctaat gcggtgccga caccggcatg catcatcagg ctacgcagaa agccacggaa
     6961 ctgttccaga accacgttgt aatgcttgtc gatatgacta tcgtcttgca ggtcggtgtc
     7021 ttcggtacgg gaagaggcta cgcgatgata aataccataa aaaccgactg caacgctgat
     7081 caccaccgcc acgacggtta aggcatcgag gaacgcggag aggaacgctg ccgccacgca
     7141 aaaagagagc gacagcagca ttttggagcg aatgcttaac agcaaacggg taaatatgaa
     7201 cagcaacagc tgtttcataa aatagatacc cgccaccata aacatcaata acagcaagac
     7261 ttcaagattt gccgccacct cttcacggac gtgttccgcg ctggtcatgc cgatgaatac
     7321 cgcttcgata gccaacagac caccggggag tagcgggtag catttcaggg ccatcgccag
     7381 agtgaaaata aattccgcta ccagcaacca gcccgcgacg aaagggctga tgaggaaaat
     7441 taacgggttt acgattaaga aaatgatgag ggcgagtttg taccagtcgg gggactggcc
     7501 caaaaaattg cgccatagcg cgcggcccca ggagatctcc atgatggttt cccttacctt
     7561 acaaataatc aatgatgttt ttatgtttaa acgcaaagct taacggtcag gcaggagtga
     7621 ggcaagtctt gatagtcaag gggaaagaga tgcggaaaat gaagccttga tccctttttc
     7681 ttctttttgt ctgctatcag cgtagttagc cctctggtat gatgagtcca actttgtttt
     7741 gctgtgttat ggaaatctca ctatggtcat taaggcgcaa agcccggcgg gtttcgcgga
     7801 agagtacatt attgaaagta tctggaataa ccgcttccct cccgggacta ttttgcccgc
     7861 agaacgtgaa ctttcagaat taattggcgt aacgcgtact acgttacgtg aagtgttaca
     7921 gcgtctggca cgagatggct ggttgaccat tcaacatggc aagccgacga aggtgaataa
     7981 tttctgggaa acttccggtt taaatatcct tgaaacactg gcgcgactgg atcacgaaag
     8041 tgtgccgcag cttattgata atttgctgtc ggtgcgtacc aatatttcca ctatttttat
     8101 tcgcaccgcg tttcgtcagc atcccgataa agcgcaggaa gtgctggcta ccgctaatga
     8161 agtggccgat cacgccgatg cctttgccga gctggattac aacatattcc gcggcctggc
     8221 gtttgcttcc ggcaacccga tttacggtct gatccttaac gggatgaaag ggctgtatac
     8281 gcgcattggt cgtcactatt tcgccaatcc ggaagcgcgc agtctggcgc tgggcttcta
     8341 ccacaaactg tcggcgttgt gcagtgaagg cgcgcacgat caggtgtacg aaacagtgcg
     8401 tcgctatggg catgagagtg gcgagatttg gcaccggatg cagaaaaatc tgccgggtga
     8461 tttagccatt caggggcgat aatcccttcc gtttaaagag caaccccctc aaacgagggg
     8521 ttttttgttg tttttacaga tttcccattc ttggcgggca acgttccagc aactcgatgc
     8581 tgccgtcttc gttttgctgt tcgagcatca catcaaatcc ccacaggcga tgcacatgct
     8641 tcaggacttc tttgcgcccc cgatccagcg gtgcgcgatt atgtggaata taacgcagcg
     8701 tcagcgaacg gtcgccgcgc aaatccacgt tccagatctg aatattcggc tccagattac
     8761 ttaagttata ttgcgacgat aaccggttac ggatctcccg ataaccttct tcattatgaa
     8821 tagcggaaat ctccagataa ttatgccgat catcatccag cacggtgaag aagcggaaat
     8881 cacgcatcac tttcggtgac agaaactggc tgataaaact ctcatctttg aaatcacgca
     8941 tcgcgaaatg cagcgtttcc agccagtcgg aaccggcgat atccgggaac cagtatttgt
     9001 cttcttccgt tggcgactga caaatccgtt taatatcctg gaacatggcg aacccgaggg
     9061 catacgggtt gatgccgctg taccacgggc tgttataggg gggctggaag accacattgg
     9121 tgtggctgtg caaaaactcc agcataaaac gttccgttac tttcccttca tcatacagat
     9181 ggttaaggat ggtatagtgc cagaaggtcg cacagccttc gttcatcacc tgagtctgtt
     9241 tttgcggata aaaatactgg ctcaccttac gcacaatacg caggatttca cgctgccatg
     9301 attccagcag tggcgcattt ttctccataa aatagagcag gttttcttgt ggttcggacg
     9361 gatagcggcg cgcttcagca accgttttct cttcctcgcg cttcggcagg gtacgccaga
     9421 gcatattgac ctgactttgt agatactctt cgcgactttt ctgccgggct ttctcttctt
     9481 gcagcgagat tttttgcggg cgtttgtacc ggtccacgcc gtagttcatc agcgcgtggc
     9541 acgagtccag aagccgttct acttcatcaa cgccataacg ttcttcgcac tcggtaatat
     9601 atttacgggc aaaaatcaga taatcgacaa tcgaactggc gtcggtccaa ctgcggaata
     9661 agcaattgtt tttaaagaaa gagttatgtc catagcaggc gtgtgccatc accagcgcct
     9721 gcatagtaat ggtgttctct tccatcaagt aagcgataca cgggttagag ttaatgacga
     9781 tttcataggc cagcccttgc tgaccgtgct tatacagccg ttcagtctcg ataaactttt
     9841 taccgaatga ccagtgcgga tagttaattg gcatgccgac gctggaatag gcatccatca
     9901 tctgttctga ggttatcact tcaatctggt gcgggtaggt atccagccgg tagagtttcg
     9961 ccacccggtc tatctctgcc agataaacat ccagcaggtc gaacgtccag tcgggtccat
    10021 cgctcaaacg tgtggtgtcc ttattcatag aatcgatcgt cgccatacgc gcacctcatt
    10081 gttgtcggcg ctctctgtgt ggagcacctc atttcaagca tagaacacct gttaaaaacc
    10141 gcgtcgccgg agaatttttt tctttgcgat ttcttattat cagagtgcca ctaatccgct
    10201 tctgaacgga attttatgct ggataaaaag ggcgttcagc aggagatact aaagacgcca
    10261 tattgccgca gagtcaggga gatgtgagtc agctcaccat aaaaaagccg tatgttgaat
    10321 aatattttca actgagttat caagatgtaa ttagattatt attcttttac tgtatctacc
    10381 gttatcggag tggctatgcg agttgtcata ctgggaagtg gtgtggtagg cgttgccagc
    10441 gcctggtact taaatcaggc aggacatgag gtcaccgtca ttgatcggga gccgggggcc
    10501 gccctggaaa ccagtgccgc gaatgccggg caaatctccc ccggatatgc tgcaccgtgg
    10561 gcagcaccag gtgtgccttt aaaagcgatt aaatggatgt tccagcgcca tgcgccgctg
    10621 gcggttcgtc tcgacggtac gcagtttcag ttgaaatgga tgtggcaaat gttacgtaac
    10681 tgcgacacca gccactacat ggaaaacaaa gggcggatgg tgcgtctggc ggaatacagc
    10741 cgtgattgcc tgaaagcatt gcgcgccgaa accaatattc agtatgaagg gcgtcagggt
    10801 gggacgctgc aactgttccg taccgaacaa cagtatgaaa atgcgacccg cgatatcgcc
    10861 gtgctggaag atgccggcgt accgtatcag ctgctggaat ccagccgcct ggcggaagtg
    10921 gatcccgcgc tggcagaagt agcgcacaaa ctgacgggcg gcctgcagtt acccaatgat
    10981 gaaaccggag actgccagct gtttacccag aatctggcgc ggatggcaga gcaggcgggg
    11041 gttaaattcc gctttaatac gcccgttgac caactgcttt gcgacggcga gcaaatctac
    11101 ggcgtgaagt ttggcgatga agtgattaag gccgatgcgt atgtgatggc gtttggttct
    11161 tactcgacgg cgatgctcaa aggcattgtt gatattccgg tgtatccgct gaaaggctac
    11221 tcgctgacta ttccaattgc gcaggaagat ggtgcgccag tatccaccat tcttgatgaa
    11281 acctacaaaa tcgccattac ccgtttcgat aaccgcattc gtgttggcgg aatggcggaa
    11341 attgttggtt ttaacaccga gctgttgcaa ccgcgtcgtg aaacgctgga aatggtggtt
    11401 cgcgatctct atccacgcgg cggtcatgtc gagcaggcga ctttctggac gggtttgcgc
    11461 ccgatgacgc cagacggtac gccggttgtc gggagtacac gctttaaaaa tctgtggctg
    11521 aataccggtc acggcacgct cggctggacg atggcttgcg gttccggtca gttgttaagc
    11581 gatctgctct ctggtcgcac gccagcgatc ccatatgagg atctaagcgt agcgcgctac
    11641 agccgtggat ttacgccatc acgtccgggc catttacatg gcgcacacag ctaaggaaac
    11701 gagatgaccc gtccgataca ggccagcctc gatctgcagg cattaaaaca gaatctgtcc
    11761 attgtccgcc aggccgcgcc gcacgcgcgc gtctggtcgg tggtaaaagc gaacgcttac
    11821 gggcatggta ttgagcgtat ctggagcgcg ctcggggcca ccgatggctt tgcattactt
    11881 aacctggaag aggcaataac gttacgtgag cgcggctgga aggggccgat cctgatgctg
    11941 gaaggatttt tccatgctca ggatctggag atttatgacc agcaccgcct gaccacctgc
    12001 gtacacagca actggcagct caaagcactg caaaatgcgc ggctaaaagc accgttggat
    12061 atttatctta aagtgaacag tgggatgaat cggttgggct tccagtccga tcgcgtgctt
    12121 accgtctggc agcagttgcg ggcaatggcg aatgttggcg aaatgaccct gatgtcgcat
    12181 tttgctgaag cggaacatcc tgatggaatt tccggcgcga tggcgcgtat tgagcaggcg
    12241 gcggaggggc tggagtgtcg gcgttcgttg tccaattcgg cggcgactct gtggcacccg
    12301 gaagcgcatt ttgactgggt tcggcctggc attattttgt atggcgcttc gccgtccggt
    12361 cagtggcgtg atatcgccaa taccggatta cgtccggtga tgacgctaag cagtgagatt
    12421 attggtgtcc agacgctaaa agcgggcgag cgtgtgggct acggcggtcg ctatactgcg
    12481 cgcgatgaac agcgaatcgg cattgtcgcc gcaggatacg ccgacggtta tccgcgccat
    12541 gcgcctaccg gtgctcctgt tttagtggac ggcgtgcgca ccatgacggt ggggaccgtc
    12601 tcgatggata tgctggcggt tgatttaacg ccttgcccgc aggcggggat tggtacgccg
    12661 gttgagctgt ggggcaagga gatcaaaatt gatgatgtcg ccgccgctgc cggaacggtg
    12721 ggctatgagt tgatgtgcgc actggcgtta cgcgtcccgg ttgtgacagt gtaacttgtt
    12781 gtaagccgga tcggaggcag cgtcttctgg ttgcgaaaaa atcatccatc cggctggtca
    12841 gcaactgtag ttgttaatgt gacagagcca ttgcccatga tagtgtccat taaaagaatg
    12901 gacactattt ccccggaacc tgaactcgcc gcacaggcgt tctacataaa acgcttacgc
    12961 ttcattgttg actcggagtc gacttcagat aaatcacgct tcacccttga tgcagattcg
    13021 ggttcatgtt cagattgttg attaacaggc tgcgagtcct gttgtctttc cagactcaac
    13081 aggcccaact gttgatccag gagctccagg tcctcctgta ccttcacgcg ctccaggagt
    13141 tccgggtcat ccaggaactc cagatccata aggtcctcca gctcctcgag tccttccagg
    13201 tcaacctagc cttccttgtg caaaaagtcc aaaaggtcct tcaggccctg ctgttttttc
    13261 aggccctcga cggctttgag cagcgtcggt aaattgtctt tgtcccacgg gttaccatta
    13321 cagtcaaaca ccttcaggct ctggggctgc ggcacctgta ccgttttaaa cttgttttct
    13381 ccacagtagt aaattattag tttgggcggc agccgtggtg aagagatcaa atcgttacta
    13441 gaacagtcaa atactttcag gcgagtcaag ttctccagca tctcatcagg tattgagtta
    13501 atattgttgc cggcacagga tagtttatac aggtactggg gcatcgacgg cggtaaaaat
    13561 tgcaagaagt tttcagaaca gttaatcact ctcaggttag tcgacagctc aggcagccag
    13621 ataatactgt tttcatagca cttcagtacc atcagttttg agggctgcac cttcggtaac
    13681 aagatcaaga cgttatctgt acagtcaagc accagcagcc actttggcat caccttcggt
    13741 aacgcggtca acacgttttt actgcagtaa aattcaatca gatgtggagg caaattctca
    13801 gggagtgaag tcaatcccag gttgctaaga ttgagcatac tccgtcttgt aatgagacat
    13861 tccttcattc tgcgcaatgc ttctgcccgg ttttcaatcc ggcttgctgg caggttttct
    13921 tccaggatat tttccttgat ttcttccaga ctttctttca ggtcttcttg gggaatttct
    13981 gactgtttgt tttgctggtt ttcttccagg atttcgtaca ggtttttttg caggttttct
    14041 tgcaaaccat cctgctgtat agcccattcg caccatttgg cgaccatcat gaactcgtta
    14101 tgttgctgat tcagacgagt ccccaacgtg atgatggtat tgttgtccac ctgtatgttc
    14161 actttgcccg cgatactgag gtcaatgatc tgccaggggg catcacccca gctgaatttt
    14221 tgcgggctga gatacttcat gttctgcaaa atttgattct tgagaagagc cgccctggcg
    14281 ggcgtcgtac gcctgggtta acggaagatg ggggaatggc gggggcgaat gggcagatgt
    14341 ctttaaagct agccataatg ctacgtttcc ttgaaataaa tatcctctgg cagcgccgga
    14401 gacgtgaatg tggtcaccgc aaaggagcca caggacgtca tttgcgctga tatcactact
    14461 catcgttgac ttttttcctg gtcagaaatg ccgtgtcggg tctggtacat gctgtattac
    14521 ggcctaggta gctctgacgt attaaagaaa atacgagagt gaacgacccg gaaataatcg
    14581 ttattaagca aaggaataac tataagaaat cgctcatgca tccggtgcta caggcggatg
    14641 aaacgcctat tgtgtgggct atgtcagtgc ggcaggttgc gtgggggaat atgttcaggc
    14701 gatgtcaggc aggggcccct gatggttgac cggtaatgcg gggctggttt ttattacctg
    14761 ttacagtcgg aaaaagccct tacgctgact ttcttgagcc ggtgttttga gttcattagc
    14821 cagagcctgc aatggtttgt catccataat ttaaccttct tctgatgttg ggatgagcat
    14881 atcaaaatca ggaaatgaca agtttattac aggctcaggc tgcgttcagt aaagcatcgc
    14941 atccggcgat agatgctgca ttaaatttaa gattcagctt cctcttcagc cacccgaacg
    15001 ccaatcttca acacttcatt atcttctttc tcggccaccg tccagatcat cccggcaaac
    15061 tctacctggt caccgacaac cggtgccgcg cctagcaact gctggacaat ttcacccagc
    15121 gtttgctgtt tatcacgata ttctcgcccg tcttccaggc catatatcag cgcaacatca
    15181 gcatatttgg cgctggcttc gagaatgaag tcaccaaaga agcgttgatc cagcgcgacc
    15241 ggtggcgact ggctgaacag tttaccgagc gccgggagat cgcgttcccg accaattaca
    15301 cacaatacat cgccttcgcg cagtcgggtg ctgccggtag gatgaagcaa ctggttatca
    15361 cgaaacaggg ctgcaatacg cgtctctttt ggcatatgca aatcgcgcag tgccgcacct
    15421 acacaccatt tatcagcact caattgataa acaaactgct cccacggatt ttccggatga
    15481 atatccaggc caacgcgtga caccggacgt ccgacgggcg gaaccaccac tttggctttt
    15541 ttcgccgccc acgagagtga tgttccctgc aagagcagtg aaaccagaac cacaaagaag
    15601 gcgacattaa agaacagtcg cgcattctcc agccccgcca tcatcgggaa cacggctagg
    15661 atgattggca ccgcgccgcg taatcccacc cagctgataa acacgcgctc acgcagattg
    15721 aaaccgcgga aagggagcaa tccagcaaat accgaaaggg gacgggcgaa gaatatcatc
    15781 catgccgaca aaatgagcgc cggaatggca ataggcagca gatcgcttgg gttaaccaat
    15841 agccccagca ccaggaacat ggcgatttgc gccagccagg cgaggccgtc gaaattttgc
    15901 aggatgccgt agcggttgcg aatcggccga ttacccagca gaaaaccgca cagataaaca
    15961 gccagaatac cgctgccttc cagcgccgtg gttaaagcaa aaattaaaat accgccgctt
    16021 aatgccagca atggatataa tccggcaggc agggcgatgc gattaatcat ttgcagcagt
    16081 aaataaccac cgccaagacc gatgacaatc ccgaggccaa attgctgcag aatatcgacc
    16141 acgaacatcc aactgacact gctttcatgc tgctggatca ttgcaatcag ggtaatcgtc
    16201 agaaagaccg ccattggatc attactgccg gattcaattt ccagcgtcga gccaacacgt
    16261 tcgttaagcc ccttaccacc cagcagagaa aagaccgctg cagcatcggt ggagccgacg
    16321 atagcgccga ttaataagcc ttcaatcaaa tcaagattaa acagccacgc cgccatcatg
    16381 ccggttaaac cagaggtgat aagtacgccc agcgtcgcca gcgacagcgc cggtcctaac
    16441 gccacacgaa aggaactggc ctgagtgcgc atcccgccgt cgagcaaaat aatcgccagc
    16501 gccaggttac ttaccatgta ggcgaagggg taattatcaa acgggatacc gccgacgcca
    16561 tcgactcccg ccagcatgcc gatcgccaga aaaataacca gaataggaat gccaagacgg
    16621 gaagaaaatg aactaagtaa tatactgctg gtgacaagga tggatcctaa gatgaaaagg
    16681 ctaattattg ttgtggcatc caatggtcgg ttactcctga ttacgctgtc tcttatataa
    16741 accctaccat attagcggca gagacagcgt tttacttagc cctgaagcgt gattttttta
    16801 cattttaaga acaggatgac cgctaatcgt caactgagcg ccttcccggg tgttattcag
    16861 aatggcatgt gcgcccaacg gcagcgtaac cgtgcgttgt tcatgaccaa aatcgaggcc
    16921 ggtaatgagt ggaatcgaca ggcgggaacg caaaaacgcg tacactgact ccaggttgta
    16981 acctgcgtca taatcattgg gcgtgctgcc gctaaagcta ccgagaataa tcgccttctg
    17041 acgtggcaaa attcccgcat gatagagctg caacagcata cgttcgacgc ggaaagggtg
    17101 cacgttaata tcttccagca ccagaatacc gttctcaatt tttggcatcc acggtgtacc
    17161 aatcagtgaa atcagcatcg caagattgcc tccccacaac gtgccttcgg tctgacatgt
    17221 cgggccttcc ccttgccatt caatggtgaa ggtttcattg cgtaacgcca gccagaaatg
    17281 gtgctcggta aaggcgttca gttcatccgc accaaagttt gctaccagca tggggccgct
    17341 aaaggtgatg acattgcctt gcgccagaag accgcactga atggcggtaa aatcgctatg
    17401 tccgcaaatg agtaacgggt catgttgttg gcgagccacc agtgcctgcc agtcaatatc
    17461 tgccagtaaa cgactggcac cgtaaccgcc gcgtacagcc agcacgatgg tgttgggggt
    17521 agtcagtctg gcaagggaat tgagatcttc cagacgctcc gtttccgtac ccgcaaaacg
    17581 ctcacaacga cgggcaataa cctcgacgtt attgacttga tgccccgcgt cggtaaggcg
    17641 ctggatacca cgcagcgcgg cgtgctgttt aatgctgtaa cccgatgggg caattaagtg
    17701 aaacagagac atggcaattc cttgctgaca acagaaacga aatgtatatc atgccgctta
    17761 ggtgtgccgt tgtcacctca acggcgattc caggctataa ggatagaaga agtgaaattg
    17821 agatggtttg cctttttgat tgtgttatta gcgggttgtt catcaaagca tgactatacg
    17881 aacccgccgt ggaacgcgaa agttccggtg caacgtgcga tgcagtggat gccaataagc
    17941 cagaaagccg gtgcagcctg gggcgtcgat ccacaattga tcacggcgat tatcgctatc
    18001 gaatcgggtg gtaatccgaa cgcggtgagt aaatcgaatg ccattggttt gatgcagtta
    18061 aaagcttcaa cctccggacg tgatgtttat cgccgtatgg gctggagtgg tgagccgacg
    18121 accagcgagc tgaaaaatcc agagcgtaat atttcaatgg gggcggctta cctgaatatt
    18181 ctggaaaccg gcccgctggc aggcattgaa gatccgaagg tactgcaata tgcgctggtg
    18241 gtgtcatacg ctaacggggc aggtgcgctg ctacggactt tctcgtcaga tcggaaaaag
    18301 gcgatcagca aaatcaacga tttggatgct gacgagttcc tcgatcacgt agcgcgaaat
    18361 catcctgcgc cgcaggctcc gcgctatatc tacaaacttg agcaggcact ggacgcgatg
    18421 taaatcagtc gcgcactttg tccgcttttt cccgggcttc tcgctcgaga gagaaaataa
    18481 tccgctgtaa ttgccgctcc accgtcgggc tgacgttaag aaaacggaag ctcagacggg
    18541 gagtggtgat ggtttcattc ttgccatcaa tcactttgcg ctcgctggtg gagattaact
    18601 gggcgtcaaa gtgaaaaaca ccccattgcc ccatgttgac ttcaatttga gcgaagcgca
    18661 taccttcttg taattcggca ggctttgctg tttccagtaa tgcgcccatg ccgcctaacg
    18721 acaaatcata caagcggaaa cgtaacgtac tgttatccgc cagtttggtc tggcaaaaat
    18781 aaggcggatg gagtggggcg gagatgcgga aatatcggcg tcgttgtaca aaccataagg
    18841 tgggaggcgg tacggtaata aatgccggaa gctgcaagta ttcactctgc tgtagttgtt
    18901 caacagtaaa ctcgactttc gcaccctgag tttcggcggt aatggtaatg tgctgtgcct
    18961 ttagcacggc gatgttgtct tcggcttgac tgccgaaatc cagcaccagt ttatccgggg
    19021 ttattgccag tattttgctg atcagctgcc cgccattcca actgagacgc aaaggaattg
    19081 cggctttgtg caaatcgcgt aacacgccca ggacggctaa cggattttgt ttcaggaact
    19141 gctcatggta atgactcacg cggaaaaact cctgactgac aaactgtctt tggtttatcg
    19201 gtcatagtta acaaaactta atacaaatgc gtgaatattt tttacatgtt gttcttaaat
    19261 cagccgacaa acgcccagca tcgatcccgc ccttgcctat acttagagcg ttgacgtaag
    19321 catttcttgc gaccgattca tcgaaacgag ggcctgaaca tgggaattat tgcctggatt
    19381 atttttggcc tgatagccgg cattatcggc aagctaatca tgccggggcg tgatggtggt
    19441 ggatttttcc tgacctgtat tctcgggata gtcggtgcgg tggtcggcgg ctggctggcg
    19501 accatgtttg gcattgacgg ctccatcagc ggttttaatt tgcacagctt cctggtggcg
    19561 gtggtgggcg ctatcctggt gctgggcgta ttccgcctcc tgcgaagaga ataagatttt
    19621 cataaggcgg atagcgatac agatgccgct atccgctttc acatcagaac gtatactcga
    19681 cacctgccca gaaattgcga ccttcttcaa taaacccttc actgtaggca tattgggtat
    19741 caaacaggtt attgaccgac gcattgacgc tgaagccatg acctaaggtg taatcggctc
    19801 gaatgtgggt caccgcaaaa ccggcggctt tttgtgaacc gtcactgttg ctgtagctgg
    19861 aggaacgcgc ctcttccgac agcgttacgc ttaacggctc ccatggtttg agtgtcatcc
    19921 atgcggtcat tgtctgcgtt ggaagatcgg ttatcttgcc gatgtctttg cgtttggcgt
    19981 cagcgtggat cagagcgtag ctcaatccta catccagtat attgctgatt ttccccttaa
    20041 taccggcatc cagaccgctg taatccaccg tgccgctgtt ctgattttga atggtatcgg
    20101 cattgatatt gtgcgagagg atggcatcac tcacccggtt atagtaaacg ctgacctcaa
    20161 atccccagtc gcgcgtgaag gcaccattcc aggttaaatc caccccgcga gcgcgttccg
    20221 gcttgagctg cgggttaaca atcgctatct ggttatacgc aggtttggat gtggtatagc
    20281 gttctttcag cgtcggaaag cgtgtgcggt catagtacga aagcgccaga gtgtcttcat
    20341 tggcaaagtg gtatttcccc atcacctgcc agttaaaagc tgactgattg ttgtcgtcat
    20401 aatgggtgat gctgccatct ttctcgtgtt ttttcgcttc tacgctatcg cgccagtcat
    20461 agctgattcc cgccacgaca tcgacattat cagcagcagc ccattgatat tcactggcga
    20521 gcgaccaggt acgatcttca tagcgatcgt aagcggcgtg cggcgcacct ttttcccggt
    20581 gtacgtcatc tttccagtta acggcaaacg acagcagatc gttttcacgc acatcggctg
    20641 ccagttgtaa tccggcaccg tcgctgtaat cggaataatg gctgtagctg ccttttttat
    20701 ttttcaaatc agccagcgag ttgtacatca tcagcgtatt ttcaaaggtg tcgcgataca
    20761 gccgactttt cagggtaaaa cggtcgttta gttgggtcgc tccctgataa taaaaacttt
    20821 ctttgtcata ctctggccac tgccagtagc gtgatttttg accacaattt ccgctgtatg
    20881 gcgggttatc tttttcacca tcctgcttaa tgtaagtcag cgtgtattca tcgttttcgc
    20941 gcggtgtaaa accgagcttc acaatgccgc gtttatcatc agccgatgaa ttaatcatct
    21001 tgccgtgttt gcctgcaata tcattattta caccatgcgg caggccgaga aaatcctgct
    21061 ttagctggct accgctgact tgcaaatacc caagatcgct gctggcggca aatgaagcat
    21121 gcatatcgta ggcattgtcc tgactacggc tccatccctg gcgatatccc agacttgctt
    21181 cttcagggtg gtgatattaa tggctccgcc catctgatta ggcccatgaa gcagcgacga
    21241 ataccctttg gaaacttcga ctgcccccag attgttggtc agaatccgcg ccagatcgag
    21301 gttgccgtca taggggacat aaatgggcac accgtcgaaa tagaccggca cctgccgact
    21361 atcaaagcca cgaactttta cctgttcttc gttgcggctg cctgactttt gcagcaccac
    21421 gccggggacg acacttaatg cctgagcgac attctgttta tccaaggcct tcatggtggg
    21481 ttgatcgaga acggtggtcg tcgtcgatga taccggactg gaccatacgg tcagcgtgtc
    21541 gctctcctga gccgctacgg cctgctgggt aaaggcaagt gataatgctg tgcagagata
    21601 acgttttttg agtctcatag aataaattcc gtttaacaaa gaattaaata gaggctgtgg
    21661 gttgcagact gactttaatg tcagctggtg ccgcgtaata aggcgcggag gtaataaaaa
    21721 ggcgaatgcc gcagtcgagg taatttttga gtgtcgtcag attaattccg ccggtcagag
    21781 ccagcgtgca gtgaggagcc agcgacggcg ctatttgcgc aatttcagtt gcctgttgcg
    21841 gactaaattt gtcgagctga agtacgtctg gttgtgcgcg taacgcggcg atagcctctt
    21901 tcggcgtgtc ggcttcaaca acaattttct tctcgggtgc gtggcgacgt aactgagcga
    21961 ttgcgcctga ccagccctga ttgtcatgaa gaaaatggcg gtggttggca aacaataata
    22021 tggtttccgc acatccggca cgatgaatta gtcctccggc agccagaatt gcctgcgaag
    22081 ccagtaagcg ggtgcccgga attgcttttc gggtgcaggc gatattgccg tcaggataac
    22141 gttcacgaag caatgccagc atttgagcga gataatcaga aacaccgcaa ctccactcca
    22201 gcacattctg gactgccttc catccctgat gaagtgctgc ggcattaccc tgcgcacgga
    22261 ttagccgttg acccgcgttc gcttgtgaac cgtcgctgac cgcgtcatca atagttaatc
    22321 ccagcgtagt taacatctta cacgcgacag aaacgccgct gacgcagccg ccctgacggt
    22381 gaaaaaacgc tatatagcca ggctggtgtc caatatttaa tgctcgcgtg gtcaggtcac
    22441 ctccctggat atcttccagc agtaacgcat cgatttgtgc ctgagaaagg aaaatcatca
    22501 cagtgcctcc tgcggtacgc tatcccacca gatgagcgcc cagtcgcggc tggcaggggc
    22561 gagggcacga gcatcttgtt gttgatacca gtgatataac cgctcacgct catcgtcgtt
    22621 aagcgcgcca agactccagc tgacgttctg ctcaaaacgt tcccaggtgc tgttatcctg
    22681 ttggcaattt tgtccacgga taaaatcaac gtgggcataa attcccatct gatacaggac
    22741 gttgagcgca aagatatagt tgggtaattc gatcacttcc cgaccagccg cccgctgaat
    22801 ggctggagag acgaacgagg tgctgaccag atgagttgta tagacgcgaa gccgtgcctg
    22861 attattcagt ttgctcattg catggcgcat atcagccacc agagtggagc gggaagccac
    22921 ggcaatatcg cagcgtggca ggtcactcca gtcctcttcc caggcgcgct ggatccagtg
    22981 gacattgtca gccttcagtg cggcggcgcg gcgggcggca acgttcagca tcccctgact
    23041 gtaatcaacg ccataaaccg tagtgagttt gtcagccagc gccagcgata cggtgcctgg
    23101 tccgcagccc atatcgaata aggtctgagc gccttgcagg tcaattttag cgatcagttg
    23161 ttgcaggtag ctgtccgttg gcgaggcgca attttccgcc attttctccg cccgttgatc
    23221 ccagtgatcg ggcgtttttt ctgtgcggtg agccagtttg agttgctgca gataaaggtc
    23281 tgcaaaatca atatcatcaa tgagcatgat tgttccttag cggctaattg cagagaggtg
    23341 atgatgaatt tggtcggcag aaacccgata cagggccgcc agtttgttcg ttgttaactg
    23401 ttcggtcggt aagccttgtg ttactcgtcc atctggctct acttggataa tgctgtcggc
    23461 aatggcgttg gcatggagcg tatggtgagt cgacattaac attgtcatgc cgttattttt
    23521 gagctgcgcc agcgtgtcca gaagttggat ctgatgaccg aaatcgaggc tggaggcggg
    23581 ctcatccagt aacaacaagc gcggttgttg gacgagtgca cgggcgatta aagccagctg
    23641 acgttcaccg ccgctaagcg tattccagcg tcgtgtcgcc agatgcaaaa tgcccagctt
    23701 ttccagttgc tccgtggctt tcaggcgttc ttgcttaccg ggaacggaaa aagcgcctat
    23761 ggtgggggcc aggcccatca acaccatatc cagcacactg aaagcaaaaa taccgtcgtg
    23821 cgcctgcggc acccaggcaa tagcctgtgc gcgctggcga tggctgagtg tctgcacaga
    23881 gaggctatcc agtaggatct cgccttttaa taacggtata acgccaagaa tggagcgcat
    23941 gagtgtggtt ttgccgcaac cattcgcgcc aagcagacaa caaattgttc cttgcgacag
    24001 ggtgaaggac acgtcgcgta gcactggatg gcgggagtaa cccagagacg catgacgaac
    24061 ggcaagcaaa gtcactggcg acctccgcgt aacaacaaaa gcaggaaaaa cggtgcgcca
    24121 acaaaagcgg tcagaatgcc aagtgggatt tctgttgttc caatgctgcg cgccagcgta
    24181 tcggtaagca ggagcagaat agcgccagta cacattgcca taggcaacag ctgctgatgg
    24241 ttatgaccgg tcaacaagcg ggcaatgtgc ggaacgacca gaccgatcca accaattatg
    24301 cccgctattg ctaccgtgct ggcggtgatt agtgtggcgc agacaatcaa tccaaagcgc
    24361 agacgcgtca cgttcagtcc cagcgaacgg gcttcgtcgt cggaaagcgt gagtaaattc
    24421 atgcgccagc ggagaaaaaa caggggcaag gagcctatga gaatgatcgg tgcagcatag
    24481 cagagatcgc gtaaggtcac ggtggacagt ccccccagca accagaaggt aattgacggt
    24541 aactgggtgt aaggatcggc cagcgtttta attagcgata tccccgctcc gcacaacgtt
    24601 cctaacgcaa tgcctaccag caccagggtt aagatcggat cgtggcgggt cacccgacgc
    24661 gtaatcaacc agacgcccgc gaccaccatc agcccgccgc aaaatgcgta cagctggatg
    24721 taaacaatgg gtaatcccca caggattgcc gcgcaggcac caagtccggc accggcggag
    24781 acgcctaaaa tatccggtga aactaaggga ttacgcaaca ttccctgata ggtggttccg
    24841 gctcctgcca gcgctgcgcc cagtaacagc gcagcaagaa tacgcggcac gcggatttgc
    24901 cagaagacaa tttgttcctg cacagactca ttttcttgac gaagaattag cgccagcagt
    24961 tgctgcatat cgagatgata agccccactt acggcggcaa aagtgatact gatagctgcc
    25021 agcagcaaaa tacatccgtt tacgattctc attagctcgc caccaatttt tgatagtcgg
    25081 catcactgag tgaacaatgc cagaacagtt gggcgtaatg ctgcatgtca cttttaaact
    25141 ggcgattgat ggcgggatcc agccaggcgt gaagtctgcg taatcccaga agacggttga
    25201 ttcccggcgg ggcatcgagc cagccaaagg gcaagccact taaaaataag atgcgttgtt
    25261 ccgcaacggc tttcacgccc tgccagagag gatcacgacg aataaaatct gcagtaacgg
    25321 cctcctgaac cagaataata tccggctgcc agcgcaggag attttccatg gaaacctgtg
    25381 tcaggccgtg gcgatcggct atttgcgcga cgttgtgcaa acctaataat tccgccgcct
    25441 cggtatgcaa cgaaccctgt aagcccgttt ccagccctcg gggaccgcgc gcagcataaa
    25501 agcggaggtt agcggcgggt gaggtggcga atgcctgagc ttcaccaacg aagtggctgg
    25561 cgagattggc ttgttctgcg gcgcggtgct cttcgcctaa cgttttgcca agcgttgtta
    25621 actgttctgc tgattgcgcc agttttccgt taagcaataa ccaggggatt tgtgtctgtt
    25681 cgctaacctg ccgtgcctgg gagatcaagg tttcatccgt attgccgcaa tcaacgacca
    25741 aatcgggatg taacgccata agcccttcca gcgatagtgt gctggcgcgc ccggcgagtc
    25801 gtcccagcct gggtaactgg cgaatgtgct ctggcaatgg aattaatgcc tgacgggcaa
    25861 aatcaaaaga ggaaaaaccg accatttttt ctggcgcgac agccagcaag agcaaatcgg
    25921 cgggcgcgcc tgcgctgacg atgcgtgtaa tgttttgggc ttcgggaaga gattgcgaag
    25981 caaaacgatt aactgtttgt gaaaaggacg gtagagactg caggagcagg gtagatgcaa
    26041 gtgcctgcgc aaacgtccgt ctggttatgg gcatggtgaa catcctgaaa agtaaaatcg
    26101 atatatactt tcatatatat cgataaaaat catgttcaat atcaaagttt agctattgaa
    26161 aacatgaaga tgtgacgact tccgcaagcc tgttgaccct ctctatcatt aaaaatgtat
    26221 ttatcaggtg gctaatatta tttaaatcat ttaaggctaa attatttaaa tatgaatgac
    26281 attattgttt ttttatcgtc cacggttacg cgacg
//
LOCUS       DAJTQQ010000054        25665 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000054 DAJTQQ010000000
VERSION     DAJTQQ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25665
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.054"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO309_002419"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO309_002419"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR5847534.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO309_002420"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO309_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR5847535.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO309_002421"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO309_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR5847536.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3807..3995
                     /locus_tag="OO309_002422"
     CDS             3807..3995
                     /locus_tag="OO309_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR5847537.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO309_002423"
     CDS             4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO309_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR5847538.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4756..5115
                     /locus_tag="OO309_002424"
     CDS             4756..5115
                     /locus_tag="OO309_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR5847539.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5112..7373
                     /gene="bcsA"
                     /locus_tag="OO309_002425"
                     /pseudo
     CDS             5112..7373
                     /gene="bcsA"
                     /locus_tag="OO309_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            7384..9723
                     /gene="bcsB"
                     /locus_tag="OO309_002426"
     CDS             7384..9723
                     /gene="bcsB"
                     /locus_tag="OO309_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR5847540.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNILPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO309_002427"
     CDS             9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO309_002427"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR5847541.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10913..11254
                     /locus_tag="OO309_002428"
     CDS             10913..11254
                     /locus_tag="OO309_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR5847542.1"
                     /translation="MDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRRSIDDIER
                     SLQNDRLAQQAEVLENQGKWAQAAALQRQRLALGPGSVWITYRLAGRSCAAIQRWRQQ
                     SGLRLHGAGIT"
     gene            11169..11420
                     /locus_tag="OO309_002429"
                     /pseudo
     CDS             11169..11420
                     /locus_tag="OO309_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCSC C-terminal domain-containing protein"
     gene            11502..13490
                     /gene="hmsP"
                     /locus_tag="OO309_002430"
     CDS             11502..13490
                     /gene="hmsP"
                     /locus_tag="OO309_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR5847543.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            13681..14967
                     /gene="dctA"
                     /locus_tag="OO309_002431"
     CDS             13681..14967
                     /gene="dctA"
                     /locus_tag="OO309_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR5847544.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            15188..16684
                     /locus_tag="OO309_002432"
     CDS             15188..16684
                     /locus_tag="OO309_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5847545.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(16780..17709)
                     /gene="kdgK"
                     /locus_tag="OO309_002433"
     CDS             complement(16780..17709)
                     /gene="kdgK"
                     /locus_tag="OO309_002433"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR5847546.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17941..18708
                     /gene="pdeH"
                     /locus_tag="OO309_002434"
     CDS             17941..18708
                     /gene="pdeH"
                     /locus_tag="OO309_002434"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR5847547.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18778..20838
                     /locus_tag="OO309_002435"
     CDS             18778..20838
                     /locus_tag="OO309_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5847548.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(21020..22342)
                     /locus_tag="OO309_002436"
     CDS             complement(21020..22342)
                     /locus_tag="OO309_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR5847549.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22753..23766)
                     /gene="yhjD"
                     /locus_tag="OO309_002437"
     CDS             complement(22753..23766)
                     /gene="yhjD"
                     /locus_tag="OO309_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR5847550.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23815..24714)
                     /locus_tag="OO309_002438"
     CDS             complement(23815..24714)
                     /locus_tag="OO309_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5847551.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            25234..>25665
                     /locus_tag="OO309_002439"
     CDS             25234..>25665
                     /locus_tag="OO309_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5847552.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSA"
BASE COUNT         6107 a         6451 c         6983 g         6124 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaaatc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag atgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1681 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaacaatga
     3061 aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tattttgcca
     3181 tgcgttattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggcc aaatagtata gacctttttc atgatttagc attgaaaata attttatttt
     3301 ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc
     3541 attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc attgaaatta
     3601 aaacttatca gatttagcat gtgaatcaat ttagaaggtc acaagacgtg tgattttagt
     3661 cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag caaattcccg
     3721 tctcgccgta aacaataaac cggcgaaatg ctcacggtta gaattgtctc atgaatggta
     3781 cggttatttc atagggatca agcaaaatga ataacaatga accagatact ctgcctgatc
     3841 ccgcgatagg ctatatcttc cagaatgata ttgtggcgtt aaagcaggca ttttcactgc
     3901 ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca ttaaaacgct
     3961 ggccgttgct ggcagagttt gcgcaacaaa agtaggggat tggtgaatgg ccgtactggg
     4021 attgcagggg gtgcggggag gcgtggggac aacaaccatc accgccgcat tagcctggtc
     4081 attacaaatg ttgggagaaa atgtcctggt ggtcgatgcc tgcccggaca acttgttgcg
     4141 cctgtcattt aatgttgatt ttacccaccg tcagggctgg gccagagcga tgctggatgg
     4201 ccaggactgg cgtgacgctg ggttgcgcta cacctcgcag ctcgatttgc tgccttttgg
     4261 tcagttatcc attgaagaac aagaaaatcc acagcactgg caaacccggc tgagcgatat
     4321 ttgctccggc ttacagcaac taaaagccag cgggcgttac cagtggattt taatcgactt
     4381 accgcgtgat gcctcgcaga taacccacca gctgctgagt ttgtgcgagc actcgctggc
     4441 aatcgtcaat gtggatgcca actgccatat ccgactgcat cagcaagcgc tgccggatgg
     4501 cgcacatatt ttgattaatg acttccgtat tggcagtcag gttcaggacg atatttacca
     4561 gctttggttg caaagccagc gccgattact gccgatgctc attcatcgtg atgaagcgat
     4621 ggctgaatgc ctggcggcta agcaaccagt aggtgaatat cgcagtgatg cgctggcggc
     4681 tgaagagata ctgacgctgg cgaactggtg cctgttgaac tactccgggc tgaaaacgcc
     4741 agtcgggagt gcatcatgag tatcctgacc cggtggttgc ttatcccgcc ggtcaacgcg
     4801 cggcttatcg ggcgttatcg cgattatcgt cgtcacggtg cgtcggcttt cagcgcgacg
     4861 ctcggctgtt tctggatgat cctggcctgg atttttattc cgctggagca cccgcgctgg
     4921 cagcgtattc gcgcagaaca taaaaacctg tatacgcata tcaacgcctc gcgtccgcgt
     4981 ccgctggacc cggtccgtta tctcattcaa acatgctggt tattgatcgg tgcatcgcgc
     5041 aaagaaacgc cgaaaccgcg cagcgggcat tttcaggtct gcaaaatatt cgtggacgtt
     5101 accatcaatg gatgaacgag ctgcctgagc gcgttagcca taaaacacag catctggatg
     5161 aaaaaaaaga gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg
     5221 tcaccttctc gctgattctg gcgttaatct gcgttactca gccgtttaac ccgctggcgc
     5281 agtttatctt cctgatgctg ctgtgggggg tagcgctgat cgtacggcgg atgccggggc
     5341 gcttctcggc gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt
     5401 ggcgttacac ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc
     5461 tgctcttcgc tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat
     5521 ggccgctgaa tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg
     5581 atatctttgt cccgacttac aacgaagatc tcaatgtggt gaaaaatacc atttacgcct
     5641 cgctgggtat cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca
     5701 gggaagagtt tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc
     5761 atgaacatgc gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg
     5821 tgtcgatttt cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct
     5881 ggttcctgaa agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg
     5941 acccgtttga acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct
     6001 atggtctggt gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctgtg
     6061 cggtgattcg tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg
     6121 aagatgcgca tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta
     6181 ttccgcaggc ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc
     6241 gctgggcgcg cgggatggta taaatcttcc gtctcgataa cccgctcacc ggtaaaggac
     6301 tgaagtttgc tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc
     6361 cacggctgat cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct
     6421 atgcgccagc gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga
     6481 ccaactcgaa gatccagggc aaatatcgcc actctttctg gagtgaaatc tacgaaacgg
     6541 tgctggcgtg gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca
     6601 aatttaacgt caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct
     6661 cgcggcccta catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc
     6721 gctacttcta tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt
     6781 tctacaacct gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac
     6841 gccgatcgaa ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc
     6901 tcttctcgtg taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc
     6961 aggcgcagat tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat
     7021 acgtcttccc gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc
     7081 cgctcaccac ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat
     7141 gggcgctctg gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc
     7201 tgaagctcgg cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg
     7261 gcatattccg tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccgcc
     7321 cggagcggag cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac
     7381 gcgatgaaaa gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc
     7441 tctttcatga cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta
     7501 gccgcccaga cggaacaaaa tccgcaggtg gggcaagtga tgtcaggcga gcagggagct
     7561 gatgccccca tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa
     7621 atcgcgccgc cgccgggcag catggtgcta cgtggtatta acccgaacgg cagcattgag
     7681 tttggtatgc gcagcgatga agtggtgacg aaggcgatgc tcaacttcga atacacgcca
     7741 tcgccatcgt tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg
     7801 ggcgtgctgc cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt
     7861 aatccactgt ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag
     7921 gacgtgtgcg aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga
     7981 ctggatctga cctatcagac cctgaatgtg aagaatgacc tgtcacactt cccggtgcca
     8041 ttctttgacc cgcgcgataa ccgtactaac atcttgccga tggtctttgc gggtgcgccg
     8101 gatgttgggc tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc
     8161 tggcgtgggc agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc
     8221 tttgcaacca acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg
     8281 gtgattgaga tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt
     8341 cgtgacgaca aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc
     8401 cgtggtgaaa gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat
     8461 gcgccgaact gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa
     8521 gaacagttac aatccagcgg ccttgagcca gcagcgatta acgtttcgct aaacctgccg
     8581 ccggatctct acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc
     8641 atgccgccgg tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa
     8701 tccttcaacc tgagcagcaa acaggaggcg aaccgtctgc tgctgcgtat tccggtatta
     8761 caaggtttgc tggatggcaa aacagatgtc tctattccgg cgctgaaact gggcgcgacc
     8821 aaccagctgc gcttcgactt tgagtatatg aacccgatgc caggcggttc ggtagataac
     8881 tgtattacct tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac
     8941 ttctcgaagt attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc
     9001 ttcccattca gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaagcgcct
     9061 aacgaagcac agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc
     9121 ttcccggcga ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc
     9181 gacatcatga tcatcggtgg tatcccggac aaactgaaag acgataagca gatcgaccta
     9241 ttggtgcagg cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc
     9301 attgtgccgg acgagagcga tcgcgcggca gaaacccagt caacgctgac ctcttccggt
     9361 gcgatggcgg cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg
     9421 ctgttggcag atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc
     9481 aaacgcgcca ccatgttcgg ttcggtcgcg gtgatccgcg agtccggtat caacagccta
     9541 cgtgttggcg acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg
     9601 ctggcaaacc atccgattct gctggcggtg ctggcgacta tcagtgtgat attgctggca
     9661 tgggtactgt ggcgtctgct gcgaattatt agtcgtcgtc gtcttaaccc ggataacgag
     9721 taattgaaga tgaatgtgtt gcgtagtgga atcgtgacga tgctgttgtt ggctgccttt
     9781 agtgttcagg cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt
     9841 caggaagggc gcgtcatcga ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa
     9901 agttacggta tgttctttgc cctggcggct aacgaccgtg cagctttcga taatattctc
     9961 gactggacgc agaacaatct cgctcagggt tctttaaaag aacgtttgcc cgcctggctg
    10021 tggggcaaga aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt
    10081 gatgtctgga tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat
    10141 accgacatcg gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct
    10201 gggctgggtt ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt
    10261 tttaacccca gctacatgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg
    10321 tggaccacgc tgcgcgaaac caatcaacat ttattgctgg aaaccgcccc gaaaggcttt
    10381 tcgccagact gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca
    10441 ttgatcagca gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc
    10501 gatccgcaaa aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa
    10561 aacggttatc cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg
    10621 gtcggttttt ctgccgccat gctgcctttt ttacaaaacc gcgatgcgca ggcagttcag
    10681 cgccagcgcg tggccgataa ctttcccggc agcgatacct attacaacta tgtgctgacc
    10741 ctgtttggac aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta
    10801 cctgactggg gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc
    10861 tggccgtcat gccgatggtc gaggcagcac caaccatcag cagaccctgc ggatggacag
    10921 cggcaacact aatgccgtgc gcgggctggc gaatatttac cgccagcagt cgccagaaaa
    10981 agctgaagcg tttatcgcct cgctctctgc cagccagcgg cgcagcattg atgatatcga
    11041 acgcagcctg caaaacgacc gtctggcaca gcaggcagag gtactggaaa accagggcaa
    11101 atgggcgcag gcggcagcac ttcagcggca acgactggcg ctgggccccg gcagcgtatg
    11161 gattacttac cgactggcag gaagaagctg cgcggcaatc caacgatggc ggcagcagtc
    11221 agggcttcgg ctacacggcg cgggcattac ttgaacgacg tgttacttcc aactggtttg
    11281 ttggcacggc aattgatatc cagcaggtga aagattacgc acccagccat ttcctgctct
    11341 acgtacgtta ttccgccgcc ggatggcagg gtgacatgga tttaccgccg cagccgctga
    11401 taccttacgc cgactggtaa gttttcagat agcgcctctc ttaatgccgc tgcgatcggg
    11461 tatactcggg cggcaatctg ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt
    11521 taacaatcaa gcagatggca atggtggcag ccgttgtcct ggtgttcgtt tttatttttt
    11581 gcaccgtttt gctgttccat ctggtccagc agaatcgcta taacacggct acgcaactgg
    11641 aaagcattgc tcgctctgtc cgcgaaccct tatcttcagc tattttgaaa ggcgatattc
    11701 ccgaagcgga agctattctt gccagcatta aaccggcagg cgtggtcagc cgtgccgatg
    11761 tagtgctgcc taaccagttc caggcgctgc gtaaaagttt tattccagag cgtccggtgc
    11821 cggtaatggt tactcgcctg tttgagctac cggttcaaat ctcgctgggc gtctactcgc
    11881 tcgaacgtcc ggcaaatccg cagccaatag cctatctggt gctacaggcg gattccttcc
    11941 gtatgtataa gttcgtgatg agcactctct caacgttagt gaccatttac ttacttttgt
    12001 cgcttatcct gaccgtcgcc atcagctggt gcattaaccg cctgattttg catccgttac
    12061 gcaatattgc tcgcgaactt aacgccatcc cagcccagga gcttgttggt caccaactgg
    12121 cattaccgcg tctgcatcag gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca
    12181 accagcaatt gctgcagcgc cattatgaag aacagaacga aaatgcgatg cgcttcccgg
    12241 tgtcggattt gccgaacaaa gccttgctga tggagatgct ggagcaggtt gtcgcgcgta
    12301 aacaaaccac cgcgctgatg atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc
    12361 tgaaagaggc gcaacgagaa attctgctgc tgacgctggt ggaaaaactc aaatcggtac
    12421 tgtcgccacg tatgatcctc gcgcagatta gcggttatga ctttgctgtc attgccaacg
    12481 gtgtacagga accgtggcac gcaatcactt tgggtcagca agtactcact atcatgagcg
    12541 agcgcctgcc gattgaacgt attcaactcc gtccgcactg tagcattggc gtggcgatgt
    12601 tctacggcga tctcaccgcc gaacagcttt acagtcgcgc tatttctgcg gcatttaccg
    12661 ctcgccataa aggcaagaat cagattcagt tctttgatcc gcagcagatg gaagcagccc
    12721 agaagcggtt gacggaagag agcgatatcc ttaatgcact ggaaaatcat cagtttgcaa
    12781 tttggttaca gccacaggtc gagatgacca gcggtaaact ggtcagtgcg gaagtgttac
    12841 tgcgtatcca gcaaccggat ggcagttggg acctgccgga tggcttaatc gatcgcattg
    12901 agtgctgtgg gctgatggtt accgtcggtc actgggtgct ggaagagtcc tgtcgattgc
    12961 ttgcagcctg gcaagagcgc ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc
    13021 aactgatgca cccgaatatg gtggcggata tgctggaact gttaacccgc tatcgcattc
    13081 agccgggaac actgattctg gaagtgacag aaagccgacg tattgacgac cctcatgctg
    13141 cggtggcaat cctccgtccg ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg
    13201 gcatgggcta cgcagggctg cgtcagctgc agcatatgaa atcgttgcca atcgacgtac
    13261 tgaaaatcga caaaatgttt gttgaaggct tgccggaaga tagcagcatg attgctgcaa
    13321 ttatcatgct ggcgcagagc ctgaacttac aaatgattgc cgaaggcgtg gagactgaag
    13381 cacaacgaga ctggctggca aaagcgggcg ttggtattgc ccagggcttc ctttttgctc
    13441 gcccactccc tattgaaatc ttcgaagaga gttacctgga agaaaagtag ctaccccaaa
    13501 ctgattacaa aactttaaaa agtgctggtt tgtgcgagcc agctcaaact ttttaacctt
    13561 tttgtttcaa ttatgatcca ggtacatttc tgtgatgttg tctgggtgtt attttaaggc
    13621 cgcaggtacc cccccccccc ataaccttac aagacctgtg gttttactaa aggacaccct
    13681 atgaaaacct ctctgtttaa aagcctttac tttcaggtcc tgacagcgat agccattggt
    13741 attctccttg gccatttcta tcctgaaata ggcgagcaaa tgaaaccgct tggcgacggc
    13801 ttcgttaagc tcattaagat gatcatcgct cctgtcatct tttgtaccgt cgtaacgggc
    13861 attgcgggca tggaaagcat gaaggcggtc ggtcgtaccg gcgcagtcgc actgctttac
    13921 tttgaaattg tcagtaccat cgcgctgatt attggtctta tcatcgttaa cgtcgtgcag
    13981 cctggtgccg gaatgaacgt cgatccggca acgcttgatg cgaaagcggt agcggtttac
    14041 gccgatcagg cgaaagacca gggcattgtc gccttcatta tggatgtcat cccggcgagc
    14101 gtcattggcg catttgccag cggtaacatt ctgcaggtgc tgctgtttgc cgtactgttt
    14161 ggttttgcgc tccaccgtct gggcagcaaa ggccaactga tttttaacgt gatcgaaagt
    14221 ttctcgcagg tcatcttcgg catcatcaat atgatcatgc gtctggcacc tattggtgcg
    14281 ctcggggcaa tggcgtttac catcggtaaa tacggcgtcg gcacactggt gcaactgggg
    14341 cagctgatta tctgtttcta cattacctgt atcctgtttg tggtgctggt attgggttca
    14401 atcgctaaag cgactggttt cagtatcttc aaatttatcc gctacatccg tgaagaactg
    14461 ctgattgtac tggggacttc atcttccgag tcggcgctgc cgcgtatgct cgacaagatg
    14521 gagaaactcg gctgccgtaa atcggtggtg gggctggtca tcccgacagg ctactcgttt
    14581 aaccttgatg gcacatcgat atacctgaca atggcggcgg tgtttatcgc ccaggccact
    14641 aacagtcaga tggatatcgt ccaccaaatc acgctgttaa tcgtgttgct gctttcttct
    14701 aaaggggcgg caggggtaac gggtagtggc tttatcgtgc tggcggcgac gctctctgcg
    14761 gtgggccatt tgccggtagc gggtctggcg ctgatcctcg gtatcgaccg ctttatgtca
    14821 gaagctcgtg cgctaactaa cctggtcggt aacggcgtag cgaccattgt cgttgctaag
    14881 tgggtgaaag aactggacca caaaaaactg gacgatgtgc tgaataatcg tgcgccggat
    14941 ggcaaaacgc acgaattatc ctcttaatct caccacttat gcccgcactc ccttccccga
    15001 gtgcgggtgt ttgcgcataa ttgacccctg tgcctgaact taccacctgg gtgagttcag
    15061 gtttatttca cttcttccgt gacattttca tgttcttgcg gtctaacacg aagtgttttt
    15121 aacgtcattt agactgaccg acaacgggca gtttttttat taccaggata gttgatcagg
    15181 ggttcacatg cagggcataa aaattcgact tttagcgggc ggtttgctga tgatggccac
    15241 tgctggctat gtgcaggcag atgcgctcca gcctgatcca gcatggcaac aggggacgct
    15301 ttccaacggt ttacagtggc aagtgctgac caccccccag cgtcccagcg atcgtgttga
    15361 aattcgcctg ctggttaata ccggttcgct cgccgaaagt acacaacaga gcggttacag
    15421 tcacgccatc cctcgtattg cgctaacgca aagcggtggc cttgacgcag cacaggcgcg
    15481 ttcattgtgg cagcagggga tcgaccctaa acgcccgatg ccgccggtaa ttgtctctta
    15541 tgacaccacg ctgtttaatc tgagtttgcc caataaccgt aacgatttgc tgaaagaagc
    15601 gctctcttat ctggcaaatg ccactggcaa attgaccatc acaccagaaa ccatcaacca
    15661 cgcgctgcaa agtcaggaca tggtggcaac ctggcctgcc gatactaaag agggctggtg
    15721 gcgctatcgt ctgaaagggt caaccttgtt aggtcacgat cctgccgatc cgctgaaaca
    15781 acccgttgaa gcggaaaaaa ttaaagattt ctatcagaaa tggtacaccc cggatgcaat
    15841 gacgctactg gtggtgggaa acgtggatgc gcgctcggtt gtcgaccaaa tcaacaaaac
    15901 gtttggcgaa ctgaaaggca aacgtgaaac gccagctccg gtgccgacgc tttctccgct
    15961 gcgtgcggaa gcggtgagta ttatgactga cgcggtgcgt caggaccggt tatctatcat
    16021 gtgggatacg ccgtggcagc cgattcgtga atcagccgca ctgctgcgct actggcgtgc
    16081 ggacctggcc cgcgaggcgc tgttctggca tgttcagcaa gcgttaagcg ccagtgacag
    16141 caaagacatc ggtcttggat ttgactgccg tgtgctgtat ctgcgtgcgc agtgtgccat
    16201 caacatcgaa tcaccaaacg acaagctgaa cagcaacctt aatctggtgg cgcgtgaact
    16261 ggcgaaggtt cgcgataaag gtctgccgga agaagagttc aatgcgttag tggcgcaaaa
    16321 gaaactggag ctgcagaaac tgtttgccgc ctatgcacgg gctgataccg atattctgat
    16381 gggccagcgg atgcgttcgt tgcaaaatca ggtcgtcgat atcgcgccgg agcagtatca
    16441 gaaactgcgg caggatttcc ttaacagcct gactgtggag atgttaaatc aggatctgcg
    16501 tcagcagttg tcgaatgata tggcgttaat actgctgcag ccgaaaggcg agccggaatt
    16561 taacatgaaa gcgttgcagg cggcctggga tcaaatcatg gccccatcta ccgccgctgc
    16621 gaccacctct gtcgccacgg atgacgtaca tcctgaagtg acggatattc cacctgcaca
    16681 gtaatgcctg atggtgatga gttagcacaa ccatcaggta tttatggatg agctggatag
    16741 aggggtaaca ctttatccag ccttttgcat atgctgcgtt tacgctggca tcgcctcacg
    16801 cgggataatc gcgccgcgat actgaataac ggtacttgcg gtcaggtgcc cgcgtttcgc
    16861 cgcgttttcc gcactgccgc ccgtcaaacg taccgccaga taaccggcgc tgaaagagtc
    16921 gccagctgcg gtggtatcga tcactttttc tttcggcagt ttcaccgccg gaacatcaac
    16981 taacgcttca ccagcaatgg acaccaggca agaatccgcc ccgcgtttca ccaccacttc
    17041 tttcacgccc gcgttatggg tgcgcgcaat gacgtcttcc accggctgtt gaccccacag
    17101 cgcgtcttcg tcgtccagcg tcaggaaggc gatatccgtg cattccagca tttgctggta
    17161 cacctgctgt gtctcttctt tgctggccca caggcgcgga cgatagttat tgtcgaaaat
    17221 cacttttccg cctttggcgc ggcattcgcg cagcagggaa agcagctttt cgcggctggt
    17281 cgggcttaag atcgccaggc taatcccgct caggtagaga taatcgaaat tcgccagctc
    17341 ttcgcaaatc gccgcagact gctcgctcgc cagccagaat ttggcggcgg cttcgttacg
    17401 ccagtagtag aacgtacgtt cgccggtgct gtcggtttca atgtagtaaa ggcccggaag
    17461 acggttttcc atccgttggg tcagggaagt atcaacgttc tcgccgtgcc aggcgtccag
    17521 catctgctgg ctaaaactgt ccgttcccag cgccgttacg taatgaacgg ttaatgccgc
    17581 aggatcgacc tgacgggcga tatagacgga agtgttcagg gtatcgccgc cgaaaccgcg
    17641 cttaacgtcc gcgcctttct cggaaagctc aatcatgcat tcgccaatca cggcaatctt
    17701 tttggacata gtcgtgaacc tgatctgtaa aaaattagcg ttagtttgcg ctgtggtggt
    17761 ttgctggtca accatattaa aacagtgttc cattattttt ttgagccaga acgtattcct
    17821 gaaagattcc gttgtggagt gaggaaattt accccggcca ggcggtaaag ttctgccctt
    17881 acgcgccgat aatctttgtc gagtccgggc agcatcactt ttaaacacag gacatctttg
    17941 atgataaggc aggttatcca gcgaataagc aaccctgaag caagcatcga gagcttgcag
    18001 gaacggcgtt tttggttgca gtgtgagcgt gcttacacct ggcagccgat ctatcaaaca
    18061 tgcgggcggt taatggccgt ggagctatta acggtggtca cgcatccctt gaacccttcg
    18121 caacgcctgc cgccggatcg ctattttact gaaatcaccg tcagccatcg gatggaggtt
    18181 gtgaaagagc agattgattt gctggcgcaa aaagccgact tctttgtaga gcacggcctg
    18241 ctggcatcgg tcaatattga tggccctacg ctcatcgccc tgcgtcagca accaaaaatc
    18301 ctgcgccaga ttgagcgtct tccctggctg cgtttcgaac tggtggagca tatccgtctg
    18361 ccgaaagatt caacctttgc ctcgatgtgt gaatttggcc cgctgtggct ggatgatttt
    18421 ggtaccggga tggcaaattt ctctgcacta agtgaagtgc gttatgacta catcaaaatc
    18481 gcgcgagaac tgtttgtgat gctgcgtcag tcgccggaag gccgcacact cttttctcag
    18541 cttttacatc taatgaatcg ctattgtcgc ggggtgattg tcgagggcgt agaaacaccg
    18601 gaagagtggc gtgatgttca gaactcgccc gcattcgccg cacaaggctg gtttctttca
    18661 cgcccggcac cgatggaaac gctgaatacg gcggttctgg cgctataagc tgcctcattt
    18721 tccgccgggc tggactatct ttaggactgg cacaggaaga atatgaggaa gcgaacgatg
    18781 agcaaggcag gcaaaataac cgctgcgatt tcaggggctt tcttgttgtt gattgtcgtg
    18841 gcgatcattt tgattgcaac ttttgactgg aatcgactca aaccgaccat caaccagaaa
    18901 gtctctgcgg agttgaatcg tccgttcgct atccgtggca atctgggcgt ggtgtgggag
    18961 cggcaaaaac aagaaactgg ctggcgcagc tgggtgccgt ggcctcatgt acatgcagaa
    19021 gacatcattc ttggtaatcc accggatatt cccgaagtca cgatggtgca tttgccgcga
    19081 gtagaggcaa cgctggcacc gctggcgctg ctgaccaaaa cggtctggct gccgtggatc
    19141 aagctcgaaa agcccgacgc gcgcctgatt cgcctctctg aaaagaacaa taactggacg
    19201 tttaatcttg ccaacgatga caacaaagac gcgaatgcaa agccgtcggc atggtcgttt
    19261 cggctggata atattctttt cgatcaaggg cggatcgcca ttgatgacaa agtaagcaaa
    19321 gcggatctgg aaatttttgt tgatccgtta ggcaagccgc tgccattcag cgaagttact
    19381 ggatcgaaag gtaaagcgga taaagaaaag gtgggcgatt acatttttgg cctgaaggcg
    19441 cagggacgtt ataacggtga accgctcacg ggtacgggaa aaataggcgg tatgctggcg
    19501 ctgcgtggcg aagggacgcc gtttccggta caggctgatt tccgttcagg taatacccgt
    19561 gttgcttttg atggcgtcgt gaatgaccca atgaagatgg gcggtgtcga tttacggctt
    19621 aaattttctg gcgattcact gggtgatctc tatgaactga cgggcgttct gctgcccgat
    19681 accccgccgt ttgaaacgga tggtcggctg gtagcgaaaa tcgacactga aaaatcgtcg
    19741 gtctttgatt atcgcggttt taatgggcga attggcgata gcgatatcca cggttctctg
    19801 gtctacacca ccggaaagcc acgaccaaaa ctggaaggtg atgtcgagtc gcggcaattg
    19861 cggctggcgg acctgggacc gttgattggc gttgattccg ggaaaggggc agaaaagtcg
    19921 aaacggtctg aacagaagaa gggcgaaaaa agcgttcagc ctgcgggcaa agtgctgcct
    19981 tatgaccgct tcgaaaccga taaatgggac gttatggatg ccgatgttcg cttcaaaggg
    20041 cggcgcattg agcatggcag tagcctgccg attagcgatc tttctactca tatcatcctc
    20101 aaaaatgctg acctgcgcct gcaaccgctg aaatttggca tggcgggcgg cagcattgcg
    20161 gcgaatattc atctggaagg cgataaaaag ccgatgcagg ggcgggcaga tattcaggct
    20221 cgtcgactga aactgaaaga actgatgccc gatgtggaac tgatgcagaa gacgctgggg
    20281 gaaatgaacg gtgacgcgga actacgcggt agcggtaact cggtggcggc gcttttaggc
    20341 aacagtaacg gcaacctgaa actgttgatg aatgacgggc tggtgagccg caacctgatg
    20401 gagattgttg ggctgaatgt cggcaactac attgtcggtg cgatatttgg tgacgatgag
    20461 gtgcgggtga actgcgcggc ggcgaatctg aatattgcca acggcgtggc gcgcccgcag
    20521 atttttgctt tcgatactga gaacgcgttg attaatgtta ccggcacggc aagttttgct
    20581 tcggaacagc tggatttgac tattgatccg gagagtaaag ggattcggat tatcacactg
    20641 cgttcgccgc tgtatgtgcg tgggacgttt aaaaatccgc aggctggggt gaaagccgga
    20701 ccgctgattg cccgtggtgc tgttgctgcg gcactggcaa cgctggtaac accggcggcg
    20761 gcgttactgg cactgatctc accttccgaa ggggaggcta atcagtgtcg gacgatattg
    20821 tcgcagatga agaagtgatg ataattgccg gatgtctgac atccggcaat taaacagatt
    20881 tagatttttg gcaaagtgtg ctttgttcat gccggatgcg gcgtgaacgc cttatccggc
    20941 ctacaaatcc ttgtgaattc aatatattgt atggaaattc agtcctgata agcgtagcgc
    21001 atcaggcttt tcacacttat tacaacgact gatgtcgcgt ctcatgggtc agcagcaggg
    21061 cgattaacgt caagccagcc atcgccgcca gatataaccc caccgcacct aacccgtagt
    21121 tagcctgcaa ccaggctgcg atatacggcg caacggaagc cccgagaatc gacgatacgt
    21181 tgtaagagaa cgatgctccg gtgtaacgca cttctgtcgg aaacagctct ggtaacagcg
    21241 cacccatcgg cccgaaggtc agccccatca gacttaaccc cagcagcagg aaggcaaaaa
    21301 ccagaatcgg gttgccagaa ccgagcagcg ggttaaaggc gaacagcgcg aacaggatga
    21361 tcagcgtggt gatgatcacc atgcttttac ggcgaccaaa ggcatcagcc agtaatccgg
    21421 cgactggcac catcacgcca aaaccaataa ctgccatcat cagcatccac aacacttcgt
    21481 tacgcggcag gccaagccca actggcgcgg cggcggtact gaaggtcata gagtagaccg
    21541 tcatgatgta aaacagcgta taggttgcca gcataatgaa cgtacccagt acggttacgc
    21601 gaacatgttt ggtcagcagc gtacccagcg ggatcttcac ctgttttttc gctttagcga
    21661 ctttctcaaa caccggcgac tcatgcagcg acacgcgaac atacaggccg ataatgacca
    21721 gcaccgccga gaagataaat ggcacacgcc agccccagct cataaactgc tcatcggtca
    21781 gtagccagga aagcagcaag aaagtgccgt tggcaaagaa gaagccaatc ggtgcgccta
    21841 gctgtgggaa ggagccatac agtgcacgtt tgcgcggtgg ggcgttttca gtcgccagca
    21901 gcgccgcgcc gccccattca ccgcctaagc ccagaccctg accaaatcga gccagcgcca
    21961 gcagcagtgg ggcgaaaata ccaatcgtgg catagcccgg cagcagacca atcaccacgg
    22021 tcgaaatccc catcgttagc aacgaggcga ccagcgtcgc tttacgccca acgcgatcgc
    22081 caaaatgacc aaaaacggca gagccaatgg ggcgcgcgac gaaggcgatg gcgaaggtgg
    22141 cgagcgactg tagcgttgct gccgttggat cgccctgcgg gaagaagata tgcggaaaca
    22201 caataacggc cgcagtggcg taaatgtaaa agtcgaagaa ctcaatggcc gtgccaatga
    22261 gagaggcgac aaggacttta ttacgcgagt tgatcggcgt gtattcttgt tcgtggtcga
    22321 gtgttgtggc tgttgcttgc ataatctttt cttatttttt ggctaacgaa tagccatatt
    22381 acgcacagca aaagtgatat ttcaatgtgt aacaaatcgc ggaaatggtc aaaaaccagg
    22441 caaaaagggg ataatgttta ggccagtgat ttttcttcta tgaaatgctt cacataattt
    22501 tataaatagt ggataaaact ggtttcaggt taaataaaag ttaccaactg gatttatatg
    22561 ctggggttat gagttggagt gaaattgttt gctcccaaat agatggctga agaggctctg
    22621 gggaagagtt tcaagaggtt atgtgcgcat tatagccgga tgcgatgctg gcgcatctta
    22681 tccggcctac ggggagccag gccgaataaa atcgtatgcc ggataaggcg tttacgccgc
    22741 atccggccta ttttaaggct gcgttttccc tggcattcgc gggtcgtctt tatattcggc
    22801 ggtggcaatc cacgccgcgc aaaacagcgt caaacgggcg aagaagtaga aaaatgccat
    22861 cagccccagc acggaaccaa atgctgcgcc agagggggat ttcatcagcg atggcagggt
    22921 gtaggtcatc acgattttaa tcacttcaaa accaatagca gccagaaatg ttccgcgaat
    22981 cagggctttt ttgcgcggac ggtggcgcgg caaccgccag aagatccaga agaaaagcag
    23041 atagttggcg aagatggaaa tcgccaggcc aatcaatcgc cacgtcggtt tcagccactc
    23101 aatgctgttc aggtgcaggg cgctaataat catttgctgc gccgaaccgg caaccgaggt
    23161 gatcgaaagc gtcacaatca gcgcaatcaa caaaccaatc agtgaaataa aatcacgcag
    23221 atattttacc cagaacttct cctgatcttg cggcgagcgt tcccagacat cgcgcgactg
    23281 ggcacgaatc gcttcacgca ggttacccat ccagttgatg ccggaataaa gtgccaccgc
    23341 caggccgaca agccctacag tcgtacgctg ctgaacggcg gtgttgatgg tgtttttcaa
    23401 cgtggcggct agtgtcggat cgctgatgtt ttgcagaatt ttgtcgaaga tatcctgtag
    23461 caacatcgga tgggaggcca gcacaaagcc ccctgcggca aacgacacca tcaaaatcgg
    23521 gatcatcgac aaaaatgaga aataggtgat agccgcacca aactggttac ccagccgatc
    23581 gttaaagcgt tctgtcgcgc gaataaggtg cgcaataacc ggttggcgtt ggactttttc
    23641 ggcagtggtg gtgacggttt ccagcgcctg gctaactttg ccgcctttct cgctattatc
    23701 caactgctta atcggctcgt gctccagatc ctgggtggga cgtttgatct cgttttcctg
    23761 cgtcatccgc tcttttgtcc ttctgtcgtg gtcattctta aaaagtatag tcggtcagtc
    23821 caggtgctct ttcattacgc cagccagcca ctgcataaac aggtttacac gccgggaaag
    23881 ctcccgacgc tgcgggtaaa ccagcgaaag ggagagcggc tcggcacggt agcctggcaa
    23941 cacttcaata agccgcccgg cacgcagggc ttcgcgcacg gcaatgcgcg ggatctgaat
    24001 aatccccaga ccggcaagac cggcggcgag atagttttcg ctactgttta ccgtcaacat
    24061 gccgccagac ttaaaccact ggacgccatt aacgctggca acctcaaaac ctaacggatg
    24121 tacacccagg tgcggtgtat aacgcactat tgcgtgtgaa gtcagatcat cggggctttg
    24181 cggataacca aagcgcgtca gatagtgcgg actggcacag ttgaccatgg ttagtttgcc
    24241 gaggggacgg gcgataacgc catcttccgg taacgcgcca gtgcgtatca cgcaatcaaa
    24301 accatcatga agaatatcta ccggacggtt atggctactc agttccagct caattcccgg
    24361 atgctgatag agaaattccg acagacgcgg cagtaacagg tttttcgcga ttcctgacgg
    24421 gatgtcgatg cgtaatttac cgctgatact ggtggcatcc tgttgaaaca gaccgtccag
    24481 ttcgctgaga ttactcaaca catcttttgc ccgttgataa taggtcatgc cttctggcgt
    24541 gagtttgacc cgtcgcgtag tgcgctggag aagctgggtg ccaagttgat gttccagtgc
    24601 ctgtatctgg cgcgaaacac ttccctttgg caaagcaaag aaatccgccg cgcgggaaaa
    24661 actttccagc tccgcgactt tgatgaacaa ctgcattgcg tgaattttat ccatttcttt
    24721 gaccgattgt tgttcacagc gaaacagtaa cgcgcatatc gcattgttta ttgttttttc
    24781 taacatctaa tgccgggttt cacaatgttg tcatgacgaa tgtggcgatt tctcagcagg
    24841 aaaagggcgg aatagcttta tgagaaaggt gaatcccctt aaacagagcg tatttttcag
    24901 tgattccctt taaagtgaat agaggacgta ttggctattt gtgcttattt ttgtctcatt
    24961 gccatttgtt attttggctt gtagtcgcgg aatttcattt ttaattgcat gcgtactaac
    25021 caccaatata aggaaatcct caaaattgtt ttaaatagtg ccctcgacat gtaatttcag
    25081 gcgtttagct gtaactattt atatcggtaa ctattttcta cgcgtcatct ctgtagaatg
    25141 gccaccgcca aatggcgaat tcttattttt tctcgcgggt aatacccttc cactacttaa
    25201 gtctttttaa cgtattcagg tggagattca gttatgcaaa tagtcatgtt tgacaggcag
    25261 tcaatattta ttcatggaat aaaaatcagt ttacagcagc gtattccagg agtgagtatt
    25321 cagggggtca gtcaggcaga cgagttatgg caaaagctgg aaagttaccc tgaagcctta
    25381 gttatgctcg atggcgatca ggatggtgag ttttgctact ggttgctgca aaaaaccgtg
    25441 gtgcagtttc ctgaggttaa ggtgttaatc acggcgacgg attgcaataa acggtggtta
    25501 caggaagtta tccattttaa tgtgctggcc attgtgcctc gcgattcaac cgtcgagaca
    25561 tttgcgttgg cggtaaatag tgcggcgatg gggatgatgt ttctgcctgg agactggcgg
    25621 acgacgccgg aaaaggacat taaagatctc aaatcgttaa gcgcc
//
LOCUS       DAJTQQ010000055        25514 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000055 DAJTQQ010000000
VERSION     DAJTQQ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.055"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..1223)
                     /gene="yeaG"
                     /locus_tag="OO309_002440"
     CDS             complement(<1..1223)
                     /gene="yeaG"
                     /locus_tag="OO309_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR5847553.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEGIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYR"
     gene            1659..2405
                     /gene="mipA"
                     /locus_tag="OO309_002441"
     CDS             1659..2405
                     /gene="mipA"
                     /locus_tag="OO309_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR5847554.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2495..3256
                     /locus_tag="OO309_002442"
     CDS             2495..3256
                     /locus_tag="OO309_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR5847555.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3307..4191)
                     /locus_tag="OO309_002443"
     CDS             complement(3307..4191)
                     /locus_tag="OO309_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR5847556.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4275..5270)
                     /gene="gapA"
                     /locus_tag="OO309_002444"
     CDS             complement(4275..5270)
                     /gene="gapA"
                     /locus_tag="OO309_002444"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5847557.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5612..6025
                     /gene="msrB"
                     /locus_tag="OO309_002445"
     CDS             5612..6025
                     /gene="msrB"
                     /locus_tag="OO309_002445"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR5847558.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6067..6339
                     /locus_tag="OO309_002446"
     CDS             6067..6339
                     /locus_tag="OO309_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR5847559.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6709..7785
                     /locus_tag="OO309_002447"
     CDS             6709..7785
                     /locus_tag="OO309_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5847560.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7812..9191
                     /locus_tag="OO309_002448"
     CDS             7812..9191
                     /locus_tag="OO309_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5847561.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9208..10253
                     /locus_tag="OO309_002449"
                     /pseudo
     CDS             9208..10253
                     /locus_tag="OO309_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10274..11110
                     /locus_tag="OO309_002450"
     CDS             10274..11110
                     /locus_tag="OO309_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR5847562.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11115..12062
                     /locus_tag="OO309_002451"
     CDS             11115..12062
                     /locus_tag="OO309_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCR5847563.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12072..13052
                     /gene="ydjG"
                     /locus_tag="OO309_002452"
     CDS             12072..13052
                     /gene="ydjG"
                     /locus_tag="OO309_002452"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR5847564.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13189..13947
                     /locus_tag="OO309_002453"
     CDS             13189..13947
                     /locus_tag="OO309_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5847565.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14065..15423
                     /locus_tag="OO309_002454"
     CDS             14065..15423
                     /locus_tag="OO309_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5847566.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15516..16157)
                     /gene="pncA"
                     /locus_tag="OO309_002455"
     CDS             complement(15516..16157)
                     /gene="pncA"
                     /locus_tag="OO309_002455"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR5847567.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16168..17184)
                     /gene="ansA"
                     /locus_tag="OO309_002456"
     CDS             complement(16168..17184)
                     /gene="ansA"
                     /locus_tag="OO309_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR5847568.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17351..19207)
                     /gene="sppA"
                     /locus_tag="OO309_002457"
     CDS             complement(17351..19207)
                     /gene="sppA"
                     /locus_tag="OO309_002457"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR5847569.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19368..19919
                     /locus_tag="OO309_002458"
     CDS             19368..19919
                     /locus_tag="OO309_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR5847570.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20036..21079
                     /gene="selD"
                     /locus_tag="OO309_002459"
     CDS             20036..21079
                     /gene="selD"
                     /locus_tag="OO309_002459"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR5847571.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21084..23045
                     /gene="topB"
                     /locus_tag="OO309_002460"
     CDS             21084..23045
                     /gene="topB"
                     /locus_tag="OO309_002460"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR5847572.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23172..24212
                     /locus_tag="OO309_002461"
     CDS             23172..24212
                     /locus_tag="OO309_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5847573.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24329..>25514)
                     /gene="gdhA"
                     /locus_tag="OO309_002462"
     CDS             complement(24329..>25514)
                     /gene="gdhA"
                     /locus_tag="OO309_002462"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR5847574.1"
                     /translation="LVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRF
                     HPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMTELY
                     RHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGY
                     GLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDE
                     SGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDV
                     DAAHQLIANGVKAVAEGAYMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQN
                     AARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQG
                     VI"
BASE COUNT         6480 a         5931 c         6586 g         6517 t
ORIGIN      
        1 tcacgatatt cctgatacga cttggctttc ggatcggtgt ctttcagact ttcgccatca
       61 taaacccgca tctttgaata aatgctggag ttttctggct ctttcaggcg cgaaagaatg
      121 gaaaaacgtg acagtgtttc gagcgtgcca ggggcgcatg gggcgtgagt caattcactg
      181 tgattaagca atttctcgta gattttgatc ccttcggaaa tgcgcaagca atacggcacc
      241 ttcacgatgt aaacacggtc gaggaaggct tcgttgtttt tgttattacg gaaagtgacc
      301 cattcggact tgttcgagtg ggcgagaata atcccgttga acggcagggc ggagatacct
      361 tccgtcccgt tgtagttacc ttcctgagtg gcggttaaca agggatgcag cacttttatc
      421 ggtgctttaa acatctcaac gaattccatg atcccctgat tggcgcggca cagcgcaccg
      481 gaatagccgt aggcgtccgg gtcattctgc gcgtagtgtt cgagtttacg aatatcgact
      541 ttcccaacca gcgcggagat gtcctggttg ttctcatcac cgggttccgt tttggcgata
      601 gcaatttgtt gcagaattga cggccagacc ttcactaccc ggaacttagt gatatcgcca
      661 ccaaattcat gcaggcgttt tgccgcccat ggcgacatga tggtgccgag ataacggcga
      721 ggaatgccat actctttttc cagaatctga gcatcttcct gcggattgaa aagacagaac
      781 ggatgatcgt tgaccgggct acgctcaccg ttggcgctca atacataaat cggtacgagc
      841 tgcattaatg atttcagtcg ctcagcaagc gatgatttac ccccacccac aggccccagc
      901 agatacagga tttgtttctt ctcttccagc ccctgagccg cgtgtttcag ataagagaca
      961 atctgttcaa tcgcgtcttc catgccgtaa aactcttcaa atgccggata acgtgcaatg
     1021 acccggttag aaaagagtcg agaaagtctg gggtcctggg ctgtatcgac catgacaggc
     1081 tcaccgatag ccatcaatag ccgctcagcc gcgttggcat aagcactgcg atcttgccga
     1141 caagtggtaa gaaactcctg cagtgtgaac tcttcgtcct tggcagcttc atatcgctgg
     1201 cgatagtgat cgaatatatt catggtatgc cgtcctttcg ttttttagca caggttaaga
     1261 gccgttcata tgaatagtag aggctcccgg aagaggtaaa ctgaacgacg tctcttgcta
     1321 aaacacagca actctcatgc cattttatcg acgatgagaa caacgtgccc ggtgatacac
     1381 cttcaaaatt aagcgtagat ggcaaatgga aaacttgcct gcacggcttt cttaatttca
     1441 atggcatatc aacaactcat ccataaattt tgcataatta atgtaaagac caggctcgcc
     1501 agtaacgcta aatccatttg gctgtaagcg cggtgtcatc cgcggcagga aaattaaaca
     1561 gttactttaa aaaatgaaaa cgtaaaaagg ttgggtttcg atgtattgac gggtaaactt
     1621 tgtcgcccgc taaacatttg tttaaggaat gattaattgt gaccaaactc aaacttctgg
     1681 cacttggagt gcttatcgca acgtctgcag gcgtagcgca cgctgaaggt aaattttccc
     1741 tgggcgcagg cgtaggtgtc gttgagcacc catataaaga ttacgatacc gatgtttacc
     1801 cagtaccggt aatcaactat gaaggcgata acttctggtt ccgtggctta ggtggtggtt
     1861 actacctgtg gaatgacgca acggataaac tttcaattac cgcttactgg tcgccgcttt
     1921 acttcaaagc gaaagacagt ggcgatcacc aaatgcgtca cctggatgac cgtaagagca
     1981 ccatgatggc tggtctgtct tatgctcact ttacccagta cggttacctg cgtaccaccc
     2041 tggctggcga taccctggat aacagcaacg gcattgtctg ggatatggcc tggttgtatc
     2101 gttacaccaa cggtggcctg accgtgactc cgggtattgg tgtgcagtgg aacagcgaaa
     2161 accagaacga atactattat ggcgtatcgc gcaaagagtc cgctcgcagc ggtctgcgtg
     2221 gctataaccc gaatgacagc tggagccctt accttgagct gagcgccagc tacaacttcc
     2281 tcggcgactg gagtgtttac ggtaccgcgc gctacacccg tctgtctgat gaagttactg
     2341 acagcccgat ggtggataaa tcctggactg gcctgatttc taccgggatc acctacaaat
     2401 tctgataatg catctttcca gggcattttg tgcataaaaa cagggcgtca cgccctgttt
     2461 tgcattacgg tggtgcaata cctgggagga aacaatgcaa caaaaaatga ttcaatttag
     2521 tggcgatgtc tcactgccag ccatagggca gggaacatgg tatatgggcg aagatgccag
     2581 tcagcgcaaa acagaagttg ctgcactacg cgcaggcatt gaactcggtt taaccctcat
     2641 tgataccgcc gaaatgtatg ccgatggcgg tgccgaaaag gtggttgggg aagcattaac
     2701 cggtctgcga gagaaggtct ttctcgtctc taaagtctat ccgtggaatg ctggcgggca
     2761 aaaagcgata aatgcatgcg aagccagttt acgccgtctc aatactgact atctcgatct
     2821 ctacttatta cactggtctg gcagtttcgc ttttgaagag actgttgctg cgatggaaaa
     2881 attgatcgcc cagggaaaaa tccgtcgctg gggcgtttct aaccttggtt atgccgatat
     2941 gcaggaactc tggcagctgc cggggggaaa tcagtgtgcc actaatcagg tgctttacca
     3001 tctcggttca cgagggattg agtacgatct actcccctgg tgccagcaac agcagatgcc
     3061 ggtgatggct tacagtccat tagcccaggc cgggcggttg cgcaatggac tgttaaaaaa
     3121 cgcggtagtc aacgaaattg cacatgtcca acaacatgca gctgcgcttg aggtcgaact
     3181 ttcgtcagcg gaattagcta tgctggataa ggcatatccg gcaccaaaag gaaaaactgc
     3241 gctggatatg gtgtgatgtt gatacgtgcc ggatgcatcg cacccggcac acaacattaa
     3301 cgcaaattaa cgtttggcaa cgcgaatgga ttgcgccaga tgtgcaggtt tctctttggt
     3361 gactttttgc gtttctgaag cgtaagccgt ttctacacaa acaaaggtct tgtagccatc
     3421 gtccggcatg tcgcccatgc taactgaaag cgccggtccc gggttccagc cgacaacgtt
     3481 cagatgatgc tggtggccta cggcgataat acgattcagc gcttcatcat taatcacgct
     3541 gcaatcttgc ggattcagat acacgcgatc ggtacggtca gggaaggtct gaataccgtc
     3601 ggtcagtaca tcttctttcg cgtcattcac tttatcaatg aagcgatcgc ccagcccact
     3661 gacgcttacc ttagcgatat cacccacgtt aaagtaggta tgcagggcag aggtggtttc
     3721 aaattcgcca tgtgattcaa gatcgatttc acaggtttta cccacgcgga aatgcgctaa
     3781 cagcgtaaag tcgtgcggcc agaatttttt cgtctcttcg ctttgtgtca attcaaaagt
     3841 cagcgctacg ccatcagcat cttcatgatg tgatttcagc gtccacggca ggttgcgggc
     3901 aaaaccgtgc gcaggcagac cttgttgtgc cgccggacca aaccacggcc agcaaaccgg
     3961 tacgccaccg cgaatagcga cgccattttt gaacggtgtg ttgttgctca accacagaac
     4021 ttcttcttca cccgcaggtt tccacgagag aaggtgtgcg ccctgtaatg caaaagaggc
     4081 ttttacctgg ggatgatcga ccacaatgag gtccagttca tccagtttac gacgggagag
     4141 gacaggggag atttgttcga tgaccggaag ggcaaaaatt ttcttaatca tgacgcagtc
     4201 ctttaacttc attttatcag gtaaaaaaaa gagcgaccga agtcgctctt tttagatcac
     4261 agtgtcatct caacttattt ggagatgtga gcgatcaggt ccagaacttt gttggagtaa
     4321 ccggtttcgt tgtcgtacca ggataccagt ttcacgaagt tgtcgttcag agcgatacca
     4381 gctttagcat cgaacacgga agtgcaaact tcgccgttga aatcggtaga tactacgtca
     4441 tcttcggtgt agcccagaac gcctttcatt tcgccttcag cagcagcttt aacggcagct
     4501 ttgatctgct cgtaagttgc agctttttcc agacgaacgg tcaggtcaac tacggatacg
     4561 ttcggggtcg gaacgcggaa cgccatacca gtcagtttgc cattcagttc tggcagtact
     4621 ttacctacag ctttagcagc accggtagag gacgggatga tgttctggga agcgccgcgg
     4681 ccgccgcgcc agtctttgtg agacgggcca tcaacggttt tctgagtagc ggtagtagcg
     4741 tgaacggtgg tcatcagacc ttcgatgatg ccgaagttat cgttgataac tttagccagc
     4801 ggagccaggc agttggtggt gcaggaagcg ttggaaacga tgtcctggcc agcatatttg
     4861 tcgaagttag cgcctttaac gaacatcgga gtgttgtctt tagacggacc agtcataacc
     4921 actttcttcg caccagcggt gatgtgttta cgagcagttt cgtcagtcag gaacagacca
     4981 gttgcttcag cgacaacgtc aacacccact tcgtcccatt tcaggttagc cggatcacgt
     5041 tcagcggtaa cacggatttt tttaccgtta acgatcagat gaccgtcttt cacttcaacg
     5101 gtaccgtcga aacggccgtg agtggagtca tatttcagca tgtatgccat gtaatcagcg
     5161 tctaacaggt cgttgattgc aacgatctcg atgtcagaac gtttctgagc agcacggaaa
     5221 acaatgcgac cgatacggcc aaaaccgttg atacctactt tgatagtcat atattccacc
     5281 agctatttgt tagtgaataa aaggttgcct gtaaaattac aaaaacctta cgcagcgtca
     5341 agcggaatcg tgtcaatcat tgcgacaaat caatcctgtg cctaagcatt acgcgactga
     5401 ctcgcctcac ttttcctttg ggcttgagac cacatggggt cggcgccccg aattttaaag
     5461 ggcaattacg ataaaaatgt gattttcatc acgatttagg tgcagccatt tcgtcgagat
     5521 caagtttagg acaaaatttt gccagttggt gttaatgttt tgttagaatc agtcacgtaa
     5581 tgtgagcacg attaaagtga gatgtgagta aatggcgaat aaaccttcgg cagaagaact
     5641 gaaaaaaaat ttgtccgaga tgcagtttta cgtgacgcag aatcatggga cagaaccgcc
     5701 atttacgggt cgtttactgc ataacaagcg tgacggcgta tatcactgtt tgatctgcga
     5761 tgccccgctg tttcattccg aaaccaagta tgattccggc tgtggctggc ccagtttcta
     5821 cgaaccggtg agtgaagaat ccattcgtta tatcaaagac ttgtcacatg gaatgcagcg
     5881 catagaaatt cgttgcggta actgtgatgc ccatctgggg catgtctttc ccgacgggcc
     5941 gcaaccaaca ggcgaacgtt attgtgttaa ctctgcttct ttacgcttca ctgatggcga
     6001 aaacggcgaa gaaatcaacg gttgaaaaaa cgattcagca acttattcca caggagcgga
     6061 aaaattatga atcttgatga cattatcaac agcatgacgc ctgaggtata ccagcgtttg
     6121 tcgaccgccg ttgaactggg gaaatggcct gatggcgttg cgttgaccga ggaacaaaaa
     6181 gagaattgcc tgcaactggt gatgctgtgg caagcccgcc ataataccga agcacagcat
     6241 atgacaattg acacaaatgg tcagatggtc atgaagagca aacagcagtt aaaagaagat
     6301 tttggtatct cagcaaagcc tattgccatg tttaagtaag tggttcatcc cctttttgcc
     6361 gcggctatga aggggatagc caacaatgac cccacgactc gtctcaatta tctcgacccg
     6421 acctggctaa gtcccagtcc gatctcttgc agtcgcgttc acgcctgaat gacttctcct
     6481 gaacttaagt ctcagaccta tttggccggt aatccctctc gaatttgctc tttttgcgtc
     6541 ccgccttcca ccaacctgta acagacccag ccattttctg cgtaattgtg atttgagttg
     6601 taaaatgcag gtcattatct gtgttgtgca gatttaatat tgacctcaaa aatcatgtgg
     6661 gtaagtatga tggccatcta agcaaaacaa aacacagagg atgacgcaat gaaagcactg
     6721 gctcggtttg gcaaggcctt tggcggctac aagatgattc atgtcccaca acccatttgt
     6781 ggcccggaag acgtagtgac tgaaattaaa gccgcggcaa tctgcggcgc agacatgaag
     6841 cactacaatg tcgatagcgg ttctgatgag tttaactcta tccgcggcca tgagttcgca
     6901 ggttgtattg cgcaggttgg tgaaaaagtc aaagactgga aagtggggca acgcgtcgtg
     6961 tcggataaca gcggtcacgt ttgcggcgtt tgtccggcct gtgaacaggg tgattttctg
     7021 tgttgtacag aaaaagtaaa ccttggtctg gataacaata cctggggcgg tggtttttcc
     7081 aaatattgtc tggttcctgg tgaaattctc aaaattcatc gtcatgcgtt gtgggaaatc
     7141 cctgatggtg ttgattatga ggacgcagcc gtacttgacc ctatctgtaa tgcctataaa
     7201 tccatcgcgc agcaatcgaa attccttcct ggtcaggatg ttgtcgtcat tggcactggc
     7261 ccactcgggc tgttttccgt acaaatggcg cggattatgg gggcggtaaa tatcgtcgtc
     7321 gttggtctgc aagaagatgt ggcggtccgc ttcccggttg caaaagaact gggggcgacg
     7381 gcagtagtaa atggttctac cgaagatgtg gtggcgcgct gccagcaaat ttgtggcaaa
     7441 gataatctgg gactggtgat tgaatgctcc ggtgccaata tcgcactgaa acaagccatc
     7501 gaaatgctcc gcccgaatgg ggaagtggta cgcgttggaa tgggcttcaa acctcttgat
     7561 ttctcgatta atgacattac cgcctggaat aaaagcatca ttgggcatat ggcctatgac
     7621 tccacctcat ggcgtaacgc tatccggcta ttagccagcg gcgctatcaa agtcaaaccg
     7681 atgatcacgc atcgtatcgg cctgtcgcaa tggcgcgaag ggtttgatgc gatggtcgat
     7741 aaaaccgcaa tcaaagtgat catgacttac gactttgatg aataactact ctcctgaagg
     7801 aacgttatcg catggaacag ataacaaaac cgcattgtgg tgcacggctg gatcgcttac
     7861 cggattgccg ctggcattcg tcaatgtttg ccatcgtcgc gtttggcttg cttgtctgct
     7921 ggagtaatgc cgttggtggc ttgatcctcg cgcagctgaa agcgttgggc tggacagata
     7981 attccactac agccacattc tcagcaatca cgaccgccgg aatgtttctc ggtgcgctgg
     8041 tgggcggcat cattggcgac aaaaccggtc gcagaaatgc gttcatcctc tatgaggcca
     8101 ttcatattgc ctcgatggtt gtcggtgctt tctcaccgaa tatggatttt cttattgcct
     8161 gccgttttgt gatggacgtt gggctgggag ctttactggt tacactgttt gctggtttca
     8221 ccgaatatat gcccggtaga aatcgcggaa cgtggtccag tcgggtttcc tttattggca
     8281 actggtcata tccgctctgc tcactgattg cgatgggact cacgccgctg attagtgcag
     8341 agtggaactg gcgggtacaa ctgcttattc ctgcaatatt gtcgcttatc gctacggcgc
     8401 tggcctggcg ctactttcct gaatccccgc gctggctgga atcgcgcgga cggtatcagg
     8461 aagccgagaa agtcatgcgg agtatagaag aaggcgtcat acgccagacg ggtaaacctt
     8521 tgccgcctgt ggttattgct gatgacggta aagcgccaca agcggtgccg tattcagcct
     8581 tactgacagg agtattactg aaacgcgtga tattaggctc ttgtgtgctg attaccatga
     8641 acgttgtgca gtacacacta attaactggt tgccaacaat attcatgacc caggggatta
     8701 atttaaaaga ctcgattgtt ttaaatacca tgagtatgtt tggcgcgcca tttggtattt
     8761 ttattgccat gctggtgatg gataaaattc cgcgtaaaac aatgggtgtg gggctattaa
     8821 tcctgattgc ggtgctcgga tatatctatt cactgcaaac cagtatgttg ctcataacgc
     8881 tgattggttt cttcctgatt actttcgtct atatgtacgt ttgctatgcc tcggcagtgt
     8941 atgtccctga aatctggccg acagaggcca aactccgtgg ctccggtctg gcgaatgcgg
     9001 tagggcgaat cagtggtatt gccgcacctt atgccgttgc agtgctgctc agtagttatg
     9061 gtgtaacggg agtctttatt cttctggggg ctgtttcaaa tattgtcgca attgctatcg
     9121 ccaccattgg aattgaaacc aaaggtgtct ccgttgaaag tttaagtatt gatgcagtag
     9181 ccaataaata atacgggtgg attcaaaatg aaaaattcaa aagcaatatt gcaggtgccg
     9241 ggcacaatga aaattatttc agcagaaata ccagtgccta aagaagatga agttttgatt
     9301 aaagtagaat atgtcggtat ttgtggttca gatgtacatg gtttttaatc aggcccgttt
     9361 attccgccta aagacccaaa tcaagaaatt ggcctgggtc atgaatgcgc cgggacggtt
     9421 gtggctgtgg gaagccgcgt gcgcaaattt aaaccggggg atcgggtaaa tatcgaacct
     9481 ggcgttcctt gcggtcactg tcgttactgt ctggaaggca aatataacat ctgcccggac
     9541 gttgatttta tggcgacaca acccaactac cgcggcgcat taacgcacta tctgtgtcat
     9601 ccggagagct ttacttacaa actgcccgac aatatggaca cgatggaagg ggcgctggtg
     9661 gagcctgccg cagtcgggat gcatgccgcg atgctggcag atgttaaacc gggtaagaag
     9721 ataattattc tgggagcagg ttgtattggt ttgatgacgt tgcaagcgtg caaatgcctg
     9781 ggcgcaacgg aaattgccgt cgttgatgta ctggaaaaac gtctggcaat aggggaacag
     9841 cctggtgcga cagtggttat taatggcgca aaagaggaca ctattgcacg ctgtcagcaa
     9901 tttaccgaag acatgggcgc agatattgtt ttcgaaacag cgggttctgc ggtcaccgtt
     9961 aaacaggcac ctttaatctg gtaatgcgcg gcggtaaaat tatgattgtt ggtactgtac
    10021 ccggcgattc ggcaatcaat ttcctcaaaa tcaatcgcga agtcactatc cagacggtat
    10081 tccgctatgc caatcgttat ccggtcacga ttgaagctat ttcttcaggg cgattcgatg
    10141 tgaaatcgat ggtgacgcat atttacgatt atcgggatgt acaacaggca tttgaagagt
    10201 cagttaacaa caaacgcgac attattaaag gcgttattaa aattagcgat taattaatac
    10261 gggagaacag aacatgctcg cagatatcag atattgggaa aacgatgcca caaataaata
    10321 ttatgcaatt gcccatttta acgtatggaa tgcagaaatg ttgatgggcg ttaaagacgc
    10381 agccgaagaa gcgaaatccc cggttattat ttcttttagt acaggttttg tcggtaacac
    10441 ctcatttgaa gatttctctc acatgatggt atcaatggca caaaaagcaa cggtgccggt
    10501 aataactcat tgggatcatg gtcggagtat tgagattatt cataacgcct ggactcatgg
    10561 tatgaattca ttaatgcgtg atgcttctgc atttgatttc gaagaaaata ttcgtttaac
    10621 caaagaggct gttgatttct ttcatccgct gggtattccg gtagaggcgg aattagggca
    10681 tgtcggtaat gaaaccgttt atgaggaagc gctggcgggc tatcactata ccgatcctga
    10741 ccaggcggct gaatttgttg aacgtacggg ctgtgactca ttggctgtcg ccatcggcaa
    10801 ccagcatggg gtttatacgt cagagccgca attgaacttt gaggtcgtca aacgcgtacg
    10861 cgatgctgtt tctgttccgc tggttttgca tggcgcatcg gggatcagtg atgccgacat
    10921 taaaactgca atttcgttgg gtattgcgaa aatcaacatt catacggagc tctgtcaggc
    10981 cgcaatggta gcagtgaaag aaaaccagga tcagcctttc ctgcatttag aacgtgaagt
    11041 acgtaaagct gtaaaagaac gggcattgga aaaaattgaa ctgttcggtt ctgatggcaa
    11101 agcggaataa tgatatggat aatctcgacg ttatttgtat aggtgccgct attgttgata
    11161 ttccattaca accggtcagt aaaaatatct ttgatgtaga ttcttatcct cttgaaagaa
    11221 tcgcaatgag caccgggggc gatgcaataa atgaagcaac aattatttct cgtctggggc
    11281 atcgtaccgc attaatgagc cgcattggta aagatgcctc agggcaattt attctcgacc
    11341 attgtcgcaa agagaatatt gatattcaaa gcctgaaaca ggacgtcagc atagatacat
    11401 ctattaacgt tggactggtg acggaggatg gcgagcggac gtttgtcacg aaccgtaacg
    11461 gcagtttgtg gaaattaaat attgacgatg ttgattttgc gcggtttcct caggcgaaat
    11521 tattatcact ggccagtatt ttcaacagtc ctctattgga tggtaaagcg ctaacagaaa
    11581 tttttacgca agcgaaagcc cggcagatga tcatttgtgc cgatatgatc aaaccgcggt
    11641 tgaatgaaac gctggatgat atttgcgaag cattgagtta tgtcgattat ctgtttccaa
    11701 attttgccga ggaaaaatta ctcacaggga aagagacact ggatgaaatt gctgactgct
    11761 ttcttgcgtg cggcgtaaaa acggtggtga ttaaaacggg taaagacggc tgctttatca
    11821 agcgtggtga catgacgatg aaggtgccgg cggtcgcagg aataaccgcc atcgacacca
    11881 ttggcgcggg cgataacttt gcttcaggtt ttattgcggc actgttagaa ggcaaaaatc
    11941 tgcgtgaatg cgcacgcttt gccaatgcaa cggcggctat ctcggttcta agcgtcggtg
    12001 ccaccaccgg cgtaaaaaac agaaagctgg tcgaacaatt gctggaagaa tacgaaggat
    12061 aatgaaggca aatgaaaaag atacctttag gcacgacgga tataacgctt tcgcgaatgg
    12121 ggttggggac atgggccatt ggcggcggtc ctgcatggaa tggcgatctc gatcggcaaa
    12181 tatgtattga tacgattctt gaagcccatc gttgtggcat taatctgatt gatactgcgc
    12241 taggatataa ctttggcaat agtgaagtta tcgtcggtca ggcgttaaaa aaactgcccc
    12301 gtgaacaggt tgtagtagaa accaaatgcg gcattgtctg ggaacgaaaa ggaagtttat
    12361 tcaacaaagt tggcgatcgg cagttgtata aaaacctttc cccggaatct atccgcgaag
    12421 aggtagaagc cagcttgcaa cgtctgggta ttgattacat cgatatctat atgacgcact
    12481 ggcagtcggt gccgccattt tttacgccga tagctgaaac tgtcgcagtg cttaatgagt
    12541 taaaggctga agggaaaatt cgcgctatag gcgctgctaa cgtcgatgct gaccatatcc
    12601 gcgagtatct gcaatatggt gaactggata ttattcaggc gaaatacagt attctcgacc
    12661 gggcaatgga aaacgaactg ctgccgctat gtcgtgataa tggcattgtg gttcaggttt
    12721 attccccgct agagcaggga ttgttgaccg gcactatcac tcgtgattac gttccgggcg
    12781 gcgctcgggc aaataaagtc tggttccagc gtgaaaacat gctgaaagtg attgatatgc
    12841 ttgaacagtg gcagccactt tgtgctcgtt atcagtgcac aattcccact ctggcactgg
    12901 cgtggatatt aaaacagagt gatttaatct ccattcttag tggggctact gcaccggaac
    12961 aggtacgcga aaatgtcgcg gcactgaata tcaacttatc ggatgcagac gcaacattga
    13021 tgagggaaat ggcagaggcc ctggagcgtt aaatactcaa cacgattcac ctctgtttct
    13081 gtgaaaactg tgatgaaaat ctgtgattca aataggttat tttgaaagta aacatcggtt
    13141 atgcgataat cgcgctaatg tgatgtaaaa agatacaggg gtgtaatcgt ggcggcaaaa
    13201 gacaggattc aggcaattaa gcaaatggtt gccaacgata aaaaggtgac agtctcaaat
    13261 ttgagtggga tttttcaggt aaccgaagaa accattcgcc gcgatcttga gaagctggaa
    13321 gatgaaggct ttttgaccag aacctatggt ggtgctgttt taaatacagc ggtgttgacg
    13381 gagaatatcc atttttataa gcgcgcttca tcgttttatg aagagaagct gcttattgca
    13441 cgcaaggcac taccctttat cgacaataaa accaccatgg cagccgattc cagtagtacc
    13501 gtaatggaat tgctcaaatt gttacaggac cgtagtgacc tgacgttgct aaccaactcg
    13561 gcggaagcga ttcatgttct ggctcagtca gaaattaaag tcgtttcaac gggtggggaa
    13621 ctaaacaaaa atacactttc cctgcaagga agaattacta aagagatcat cagccgctac
    13681 catgtcgata tcatggtaat gagttgtaaa ggtcttgata ttaacagcgg cgcgctggac
    13741 tctaatgaag cggaagctga aatcaaaaag acaatgatcc gtcaggcgac agaagttgcg
    13801 ttattggttg atcactctaa gtttgatcgt aaagcttttg tccagttagc tgactttagt
    13861 catattaatt atataataac tgataaatca ccgggtgcag agtggatagc attttgcaaa
    13921 gacaataata tccagctggt gtggtaattt aagcacacgt tgtcaatatt atttgttcct
    13981 gcgactggaa ataaaatgat gataaagatg cggcaagtga atttgccgca attgctacct
    14041 tttatagcaa atccgaggca cccaatggaa caatatgatc aaattggcgc aagactggac
    14101 cgcttgcctt tggcccggtt tcattatcgt atatttggta taataagctt tagtctgtta
    14161 ttaacggggt ttttgagtta ctcaggtaat gtcgtcttag caaagctggt aagcaatgga
    14221 tggtcaaata atttcctcta tgccgccttt acctcggcat taatgtttgg ttatttcatc
    14281 ggctcactta ctggtgggtt tattggtgac tactttgggc ggcgcagggc gtttcgcata
    14341 aatcttctca tcgtcggtat tgctgcaaca ggggccgctt ttgtccctga tatgtactgg
    14401 ctcattttct ttcgcttcct gatgggaaca ggaatggggg cgctgattat ggttggctat
    14461 gcctcattta cggagtttat ccccgcgacg gtgcgtggaa aatggtccgc gcggctctca
    14521 tttgttggta actggtcgcc catgctgtct gcggcgatag gcgtggtggt tatcgctttt
    14581 tttagttggc gaataatgtt tcttctgggt ggtattggca tactgttagc ctggtttctc
    14641 tcaggtaaat actttatcga gtcgccacga tggctggaag ggaaagggca aatcgccggt
    14701 gcagaaagcc aacttcgtga agtagagcag caaattgaaa gggagaagag tattcgttta
    14761 ccccagctta ctttgaacca gagcaacagc aaggttaagg taatcaaggg taccttcagg
    14821 ctcctgttta aaggggaaat gttacgacgt acattagttg cgataactgt tttaattgca
    14881 atgaacattt cgctttatac cattaccgta tggataccga ccatatttgt taactccggc
    14941 attgatgtcg ataaatcaat attaatgacc gctgttatta tgattggtgc tccggtagga
    15001 atatttattg cggcattaat tattgatcat tttcctcgtc gattatttgg ctccgcctta
    15061 cttattatta ttgccgtgtt aggctatatc tattcaagtc agactacaga gtgggcgatt
    15121 ttaatctatg gtctggtgat gatcttcttt ttatacatgt atgtttgctt cgcgtcggcg
    15181 gtttatatcc cggagctttg gccaacgcat ttacgcctgc gcggttcggg tttcgttaat
    15241 gccgtcggac ggatcgtcgc agttttcacg ccctatggcg ttgcggcatt attaacacat
    15301 tatgggtcga tcacggtgtt tatggtgctt ggtgtcatgt tagtgctctg tgcgctggtt
    15361 ctctccattt ttggcatcga aacgcggaag gtgtcgctgg aagagatttc tgaggtgaat
    15421 taaatctttc gttgatggca tcaattgcca gatgtaccac tgacgtgttt tatttggcct
    15481 acaatgccag tacagaattg taggccagcg taaaattacc cctgcgtctc ttcccagtct
    15541 gccagcgtat atagcgttgc cccagctgct gacatctcca taaacgcgtg tgcactgtcc
    15601 tggggctgga tattcacgcc acgacagcca tcggtaatca cgtttacctt ataacctaac
    15661 tgtaacgcgt ccagcacggt aaacttcacg caatagtcag tagccaggcc cataacgatc
    15721 aattcatcga tttcatgatc gcgcaaccag tcatcaagcg cggttttctg ccgacgtccg
    15781 ttatcaaaaa aggcactgta actgtcaact aaaggatttt cgcctttatg gaacaccgct
    15841 gcgatctctt tttgattcag taacggatgt aattgtgcgc cttcactgtt ctgcacacag
    15901 tgttcaggcc agaaagtttg ccgcaggccg ccgagttggc ctggcgtata aggctctacg
    15961 ccgtgctggc tggcaaagct gccgtgattc gccggatgcc agtcctggct ggcgataacc
    16021 gcttctccgc gcgactggca ccagtcaata aggcggttag caacctccac cgtactgtca
    16081 ccttccggta cggcgagcgc gccaccagca cagaaatcat tttgtaaatc gaccagtaac
    16141 agggcgcgat ggggcattgc agtctcctta atcatccggc gtcagttcgc cgcgcaggtt
    16201 ttggctcatg gccttgcgaa tggtttcagt atccagttcc tggctcagca ggtaatgcag
    16261 tttggttagc gtggcttcta cagtcatatc tgcaccgtca ataacgccgg catgggcgag
    16321 ggcgttaccg gtggcataac cactcatatt cactttaccg gacatacatt gtgtcaggtt
    16381 gaccaccaca ataccgcgat cgctggcttc ttgtaattcc tgcaggaagg ctttgttttg
    16441 tggcgcatta cccacgccat aggagcgcag aatcaatgct ttcaccggtt ggcgcagaaa
    16501 attgcgcacg acgtcagcag aaatccctgg ataaatcgtc accacgccaa ttggttgtgg
    16561 cgtgattgga tgaacgatta attccccttc accgtgcggg gcgggtggcg tattcaaacg
    16621 acgaatatgg ataccagctt ccagtaacgg aggaaggttt ggagaggcaa acgcatcaaa
    16681 accatcggca tgggctttgg tagtgcggtt gccgcgatac aatcggttat tgaaaaagag
    16741 cgttacttcg ttgatcggat aattcggcgc aacgtacaac gcattcagca gattaatttg
    16801 tccgtcagag cgtaactcag ccagcgggat ttgtgaccct gtcacaatga ccggtttacc
    16861 gagattctcg agcatgaacg acagcgcaga ggcggtatac gccatcgtgt cggtaccgtg
    16921 tagaatgaca aaaccatcat agtcgtcata gtgcgcttta atatcttcag caatatgctg
    16981 ccagtcttcc ggcgtcatat cagaagaatc catcaacggc gtatattcat gaatggtgaa
    17041 atctggcatc tccgggcgat ggaattccgg catcagcgcc agttggcgtt gtagatgacc
    17101 tgacaccgga atataaccct gctcggaacg ctgcatcccg atggtcccgc ccgtgtaggc
    17161 gacgtaaatt gatttctttt gcatgatatt gatatgaatg atatcgaaag agcaaaagta
    17221 tatacgtgag gcaggaggag cgcacctgat actatgcgga taaggaaata tggggcgaga
    17281 cagcgggaag aggattcgtc gaggtaaaaa ataatgtggc cgacgatctg tcggccactc
    17341 agtacaagac ttaacgcacg ttggcgcagg tcaggcaaaa cgcataacgg ttttgtgggt
    17401 cgttaaacgc ggccagcttg tcgctttcac ttttaacggt agaggctacc gaggcaagcg
    17461 gtgcaggtaa catggcctgg aacgcatctg gcaacattgc ccggacagaa ccagacatgt
    17521 tgtccatcac tttgtcgaag aaggtcggtt catcaacgta gtattccaga tgccactgtt
    17581 tcacttttgc gagctctgct gctttggcaa ccgcatcatc gaaatccccg agactatcga
    17641 ccagcccgtt agcttttgca tcctgaccgg tccagacgtg gccctgggcg attttatcaa
    17701 tttgctccgg cgtcgaatga cgcgcatcag caaccagcgt gataaagcgt ttatagccat
    17761 tctcaatgct taattgcatc atctgctgcg cttccggcgg cagtgccctg gtgatagaaa
    17821 catccgccag cggtgaagtt gagacaccat cagtatgaac accaatcgaa tccagactat
    17881 tttctacggt ggtgatcacg ccgaagatac cgatagaacc ggtcagggtg ctggggttag
    17941 ccacaatgta attagctggc gtggaaatcc agtaaccacc agatgccgcc atgccgccca
    18001 tcgatacaac cacagactta cccgctgccc gggctgctgc cagttcagcg cgaatcactt
    18061 cagacgcggt aacgctgccg cctgggctat taacacgcag gacaatcgct ttcactttcg
    18121 ggtcaaggcg agcgtcgcgg atttgtgccg cagtggtatc accgccaaca ttcccctgag
    18181 tttcctcgcc atccataatt gcgccattag cgaagacgac accgatgctg tcaccggtat
    18241 ctgccggcgt tttcaatgcg taatcgtaat aactgatggc gcgataattt ttatcagtct
    18301 tactccagcc gaactctttg gtcagagctt tttcgatttc ggcactcgat gccagtgcat
    18361 cgaccagctt gttttccagt gcatatttcg cggtatcgcc accggttttg gttaaaccct
    18421 caagcaaccc ttgcgcgcca gggaatacct gctgagcagg gatctgccgg ttagcggcaa
    18481 cagtattcag atagttttgc cacagctcac caatccagcg gctgtcagct tcgcgggctg
    18541 ccggtgacat atcatcacga ataaacggtt caacggcaga tttatacgta cccacgcgga
    18601 acacatgggt ggaaactttc agcttatcca gcaacgattt gtagtacaga ccgttggtag
    18661 caaaaccgtg cagatcaacc acgccttgcg gtgacagcca aattttattg gcgaaactgg
    18721 cgagataata ttgcccctgg ctgtagttct cgccaacggc ataaaccggt ttcccgctgt
    18781 cacgaaactc tttcagggct ttgccgatgt actgcataga cggctggtcg ccgcctgcga
    18841 agtttttcag atccatcacg ataccggtga tattgcggtc gtccttcgcc tggcgaatgg
    18901 tgttgacgat atcaaacagt gagttttcct gcagacgatc ggaactggca ccaagcagct
    18961 ggcggcttaa tttactaaac cgctgagaac tgtctggttt atcgacgatc acaccagaaa
    19021 tgtccagcag cagtgcgcca cgactggccg tttctttcga atcaccacca ctgacctgca
    19081 tccagatccc cacaccaacc agtacgagga aaataaagaa caggttaagt accatttcac
    19141 ggacgaaatt cagcagacgc cacgtccatt taaaaaatcc ggcaataaat cgccaaaggg
    19201 ttcgcatgta ttctcccaac ttaaggtcac acctgtcaca ggcgcaatag atgtggttat
    19261 cgtaatgacc caaccggcac ttgtcagcag gaattgcgga ctgtgctgta acaaaatcca
    19321 ctctcgtgtt aattttgtga ataaatatca cgacaggagt taatcaaatg gatgcactcg
    19381 aactattgat caatcgccgt agcgcctccc gcctggctga acccgcgcca acgggtgaac
    19441 aactgcaaaa catcctgcgt gcgggtatgc gtgcgccgga ccataagtcc atgcaaccgt
    19501 ggcatttttt tgtgattgaa ggggaagggt gcgagcgttt cagcgccgta ctggaacagg
    19561 gggcgattgc tgccggtagt gatgataaag ctatcgacaa agcccgtaat gcgccgttcc
    19621 gcgcaccgct catcatcacg gtggtggcga aatgcaaaga aaatcataaa gtcccgcgat
    19681 gggaacagga aatgtctgcc ggatgcgcgg tcatggcgat gcaaatggca gccgttgccc
    19741 agggatttgg cggcatctgg cgcagtggcg cattaactga aagtccggta gtgcgtgaag
    19801 cattcggttg ccgtgagcag gataaaattg tcggttttct ctacctcggc acgccgcagt
    19861 tgaaagcatc tacgtcgatt aacgtcccgg acccgacgcc gtttgtaact tatttctgat
    19921 aattgtcgcg aaactgtctg gattgtgagc aaaggcgcag gaattcagac actctcactt
    19981 atcacttcac ggaatgaggg ttaccatacc cggattgcat tgacaggaga tgtccatgag
    20041 cgagaactcg attcgtttga cccaatacag ccacggagct ggttgcggct gtaaaatttc
    20101 cccaaaagtg ttggaaacca tcctgcacag tgagcaggcg aagtttgttg atccgaattt
    20161 gcttgtgggt aatgaaaccc gcgacgatgc ggcggtgtac gatctgggca atggcaccag
    20221 cgttatcagt accaccgact tctttatgcc gatcgttgat aatcctttcg attttggccg
    20281 cattgcggcg actaacgcca tcagcgatat cttcgcgatg gggggcaaac cgattatggc
    20341 gattgcgatc ctcggctggc cgattaacaa actttccccg gaaattgccc gcgaagtaac
    20401 cgaaggtgga cgctatgcat gccgtcaggc gggtattgcg ctggctggcg gtcactccat
    20461 cgatgcgccg gagccgattt ttggtctggc ggtaacgggg atcgtaccga ccgagcgggt
    20521 gaagaaaaac agtaccgcac aagccggatg caaactgttc ctgacgaaac cgctggggat
    20581 cggcgttctt accacggctg agaaaaaatc actgttgaaa ccagaacatc agggactggc
    20641 gacggaagtg atgtgccgga tgaacatcgc aggcgcgtcc tttgccaaca tcgaaggcgt
    20701 aaaagcgatg accgacgtta cgggctttgg tctgctgggc cacttgagcg aaatgtgtca
    20761 gggggctggt gtgcaggcac gcgtcgacta tgaagcgatc ccgaaactcc ccggtgttga
    20821 agagtacatt aagttgggcg cagtacctgg cggcactgaa cgtaactttg ccagctacgg
    20881 tcatctgatg ggtgaaatgc cgcgtgaagt gcgcgatctg ctgtgcgatc cgcaaacttc
    20941 tggcggtttg ctgctggcgg tcatgccgga agcagaaaat gaggttaaaa ctacagccgc
    21001 cgagtttggc attgaactga cggcgattgg cgaactggtg ccagcgcgcg gcggtcgtgc
    21061 catggttgag atccgttaat ttaatgcggt tgtttattgc cgaaaaaccg agtctggcgc
    21121 gcgccattgc tgatgtcctg cccaaaccgc accggaaagg cgatggcttt atcgagtgcg
    21181 gtaatggtca ggtggtgact tggtgtatcg gtcacctgct tgagcaggcg cagccagacg
    21241 cctacgacag ccgctatgcg cgctggaatc ttgcggattt gccgattgtc ccggaaaagt
    21301 ggcaattaca gccacgaccc tccgtgacca aacaacttaa cgtcatcaaa cgcttcctgc
    21361 atgaagccag cgaaatcgtt cacgccgggg acccggatcg tgaagggcaa ttgctggtgg
    21421 atgaagtgct ggactatctg caactggcac cggaaaagcg ccagcaggtg cagcgttgct
    21481 tgataaacga cctgaacccg caggcggttg agcgggcgat cgaccgtctt cgttccaaca
    21541 gtgagtttgt gccattgtgc gtttctgcgc tggcgcgagc gcgtgccgac tggctgtacg
    21601 gcatcaatat gacccgtgcg tataccattc tcggtcgcaa tgccggttat cagggcgtac
    21661 tttccgtggg acgcgtgcag acacccgtgc tcgggctggt ggtgcgccgc gatgaagaga
    21721 ttgaaaactt cgtggcgaaa gacttctttg aagtcaaagc acatatcgtg acacctgccg
    21781 atgagcggtt taccgctatc tggcaaccga gcgaagcgtg tgaaccgtac caggatgaag
    21841 aagggcgctt gttacatcgt ccactggcgg agcatgtggt taaccgcatt agtggtcaac
    21901 cggctattgt caccagctat aacgataaac gggaatcaga atccgcgccg ctgccgtttt
    21961 cgctttcggc gttgcagatt gaagcggcaa aacgcttcgg tctgagcgcg cagaacgtgc
    22021 ttgatatctg ccagaagctg tacgaaacgc acaagctaat cacttatccg cgttccgatt
    22081 gtcgctattt gccagaagaa cattttgccg gacgccacgc ggtgatgaat gccatcagcg
    22141 ttcatgcacc ggatctgttg ccgcagccag tggtagatcc agatatacgc aaccgctgtt
    22201 gggatgacaa aaaggtcgat gcgcaccacg ccatcatccc gaccgcacgg agttctgcga
    22261 tcaacctgac ggagaacgaa gcgaaggtct ataacctgat tgcccgtcaa tacctgatgc
    22321 agttctgccc ggatgcggtg ttccgcaagt gtgttatcga actggacatt gccaaaggca
    22381 aatttgtcgc taaagcccgt tttcttgctg aagcaggctg gcgcacgctg ttaggcagca
    22441 aagagcgcga tgaagaaaac gacggcacgc cattgcctgt ggtggcgaaa ggcgatgagt
    22501 tgctgtgtga aaaaggtgaa gtggtagagc ggcaaaccca gccgccgcgc cattttaccg
    22561 atgcaacact gctttcggcg atgaccggga tcgcgcgctt tgtgcaggac aaagatctga
    22621 aaaaggtcct tcgtgcgacc gatggtctgg ggacagaagc aacccgtgcc gggattattg
    22681 aactgctgtt caagcgtggt tttctgacca aaaaagggcg ctatatccac tccaccgacg
    22741 ccggaaaagc gttattccat tcgctgccag aaatggcgac gcgaccggac atgaccgcgc
    22801 actgggaatc ggtgctgacg caaatcagcg aaaagcagtg tcgctatcag gactttatgc
    22861 agccgctggt ggggacgcta tatcagctta ttgatcaagc caaacgtacg ccggtgcggc
    22921 agtttcgcgg cattgtggct ccgggcagtg gtggcagtgc tgataagaaa aaggctgcac
    22981 cgcgtaaacg tagtgcgaaa aaaagtccgc cagcagatga agccggaagc ggggcgatag
    23041 cgtaagcgag cgaatctttc gtgctattcg agtcatattc tgaaatatcc agcggatcaa
    23101 gaaagttcgt tggatatttt tttgcatgga taaaattatc gcctctaaag tatgtaataa
    23161 cagggaatgt gatgaaaaaa gttttattgc aaaaccatcc tgggagcgag aagtattctt
    23221 ttaatggctg gggaatattt aatagtaatt ttgaacgggt gattaaagaa aataaggcca
    23281 tgctgctttg taagtggggg ttttatttaa catgtgttgt cgctgtaatg tttgtattcg
    23341 cagcgataac atccaacggt ttgaatgaaa gaggcctgat taccgcggga tgctcttttc
    23401 tttatctatt aattatgatg gggcttattg ttcgggccgg ttttaaagca aaaaaagaac
    23461 aactgcatta ttatcaggct aaaggtattg agccgctcag tatcgagaag ttacaggcgc
    23521 tacaattgat cgcaccttat cgattctatc ataagcaatg gtctgaaacg ctagagttct
    23581 ggccgcgaaa gcctgaacct ggcaaagata ccttccaata tcatgtgctt ccttttgact
    23641 cgatcgatat catcagtaaa agacgcgagt ctttagagaa tcaatggggt atcgaagata
    23701 gcgaaagtta ttgtgcctta atggagcatt ttctttctgg cgaccatgga gccaatacct
    23761 ttaaagcaaa catggaggaa gccccagagc aggttatcgc cttgttgaat aaatttgctg
    23821 tttttccctc agactatatc tctgattgcg ctaataatcg ctccggtaaa tcctcggcga
    23881 agctaatatg ggcggcggaa ttatcatgga tgatctcgat atcaagcaca gcttttcaaa
    23941 acgggacaat tgaagaagaa ctggcctggc attatataat gcttgcttct cgaaaggcgc
    24001 acgagttgtt cgaaagcgaa gaagattatc aaaaaaatag tctaatggga tttctttact
    24061 ggcatatctg ctgctatcgc agaaagttaa cggatgctga acttgaagcg tgttatcgtt
    24121 acgacaagca gttttgggag cactacagta agaaatgccg ttggcccata agaaatgttc
    24181 cctggggagc atcatccgtt aaatactcat aaacgcctga aattttgccg ggggcgctgc
    24241 gcttgaccgg cctacattag agcgtaactg caatgaattg tgcccatctg taggcctgat
    24301 aagcgtagcg ccatcaggca tttacaactt aaatcacacc ctgcgccagc atcgcatcgg
    24361 caaccttcac aaaaccggca atgttcgcgc cctgcacgta gttggtttgc tcaccttcac
    24421 caccatgctc aacacaggca tggtggatat ccagcatgat gtgatgcaaa cgtgcgtcaa
    24481 ctttctcggc tttccagccc aggcgcgcag cgttttgtgc catttccagg cccgacgttg
    24541 ctacaccgcc agcattagcc gctttacccg gtgcaaacag tacgcctgcc tgctggaaca
    24601 gttcagtcgc ttcgatggtg gtcggcatat atgccccttc agcgacggct ttaacgccat
    24661 tggcgataag ctgatgcgcg gcgtcaacat ccagttcgtt ctgggtggcg caaggcaggg
    24721 cgatatcaac cggtacagac cacggctgtt ggccttcgag atagaccaga ccaaattctt
    24781 tggcgtaatc tgccgctcga ccatcgcggc tggctttgat ttcgataaga cgtgccagtt
    24841 tctctttcgt gaatccgctt tcatcaacta cagtgccgct ggagtctgac gcagtgatca
    24901 cacgagcacc aaattccatc gctttttcga tagcgtactg ggcgacgttg ccggagccag
    24961 aaacggaaac gctcatccct tcaaaaccca taccgtggcg ttttaacatt gcttctgtga
    25021 aataaaccag accgtagccg gtagcttccg ggcgaataag actaccgcca aatgaaaggc
    25081 ccttaccggt gaagacgcag gcggtattgt tggagagctt tttcatcatc cccgccataa
    25141 agccgacttc acgaccacca acgccgatat cacctgccgg aacgtcggta tccgcgccca
    25201 ggtggcgata cagttcagtc atcagcgtct ggcaaaaacg catcacttta ccttcgcttt
    25261 ttcctttcgg atcgaaatcg ctgccgcctt taccaccgcc catcggcagg gtagtcaggg
    25321 catttttgaa ggtttgttca aagccgagga atttgagaat ggaaaggtta actgacggat
    25381 ggaagcgcat accgcctttg tacgggccga tggcagagct gaactgcaca cgccatgcac
    25441 ggttgacctg tatctggttg cgatcatcaa cccataccac gcgaaactgg atcacgcgct
    25501 ccggttcaac caga
//
LOCUS       DAJTQQ010000056        24647 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000056 DAJTQQ010000000
VERSION     DAJTQQ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.056"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(1..376)
                     /locus_tag="OO309_002463"
                     /pseudo
     CDS             complement(1..376)
                     /locus_tag="OO309_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            900..2828
                     /gene="thrS"
                     /locus_tag="OO309_002464"
     CDS             900..2828
                     /gene="thrS"
                     /locus_tag="OO309_002464"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR5847575.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2832..3374
                     /gene="infC"
                     /locus_tag="OO309_002465"
     CDS             2832..3374
                     /gene="infC"
                     /locus_tag="OO309_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR5847576.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3471..3668
                     /gene="rpmI"
                     /locus_tag="OO309_002466"
     CDS             3471..3668
                     /gene="rpmI"
                     /locus_tag="OO309_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR5847577.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3721..4077
                     /gene="rplT"
                     /locus_tag="OO309_002467"
     CDS             3721..4077
                     /gene="rplT"
                     /locus_tag="OO309_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR5847578.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4194..4318
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4200..4244
                     /gene="pheM"
                     /locus_tag="OO309_002468"
     CDS             4200..4244
                     /gene="pheM"
                     /locus_tag="OO309_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR5847579.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4527..5510
                     /gene="pheS"
                     /locus_tag="OO309_002469"
     CDS             4527..5510
                     /gene="pheS"
                     /locus_tag="OO309_002469"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR5847580.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5525..7912
                     /gene="pheT"
                     /locus_tag="OO309_002470"
     CDS             5525..7912
                     /gene="pheT"
                     /locus_tag="OO309_002470"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR5847581.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7917..8216
                     /gene="ihfA"
                     /locus_tag="OO309_002471"
     CDS             7917..8216
                     /gene="ihfA"
                     /locus_tag="OO309_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR5847582.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8317..9297
                     /gene="btuC"
                     /locus_tag="OO309_002472"
     CDS             8317..9297
                     /gene="btuC"
                     /locus_tag="OO309_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR5847583.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9360..9911
                     /gene="btuE"
                     /locus_tag="OO309_002473"
     CDS             9360..9911
                     /gene="btuE"
                     /locus_tag="OO309_002473"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR5847584.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9911..10660
                     /gene="btuD"
                     /locus_tag="OO309_002474"
     CDS             9911..10660
                     /gene="btuD"
                     /locus_tag="OO309_002474"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR5847585.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10738..11202
                     /locus_tag="OO309_002475"
     CDS             10738..11202
                     /locus_tag="OO309_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5847586.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11449..12162
                     /gene="ydiV"
                     /locus_tag="OO309_002476"
     CDS             11449..12162
                     /gene="ydiV"
                     /locus_tag="OO309_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR5847587.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12226..13662
                     /locus_tag="OO309_002477"
     CDS             12226..13662
                     /locus_tag="OO309_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR5847588.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13666..13857)
                     /gene="hemP"
                     /locus_tag="OO309_002478"
     CDS             complement(13666..13857)
                     /gene="hemP"
                     /locus_tag="OO309_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR5847589.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13989..15035)
                     /gene="aroH"
                     /locus_tag="OO309_002479"
     CDS             complement(13989..15035)
                     /gene="aroH"
                     /locus_tag="OO309_002479"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR5847590.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15192..16025)
                     /gene="ppsR"
                     /locus_tag="OO309_002480"
     CDS             complement(15192..16025)
                     /gene="ppsR"
                     /locus_tag="OO309_002480"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR5847591.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16357..18735
                     /gene="ppsA"
                     /locus_tag="OO309_002481"
     CDS             16357..18735
                     /gene="ppsA"
                     /locus_tag="OO309_002481"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR5847592.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18792..20438)
                     /gene="fadK"
                     /locus_tag="OO309_002482"
     CDS             complement(18792..20438)
                     /gene="fadK"
                     /locus_tag="OO309_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR5847593.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20495..20788)
                     /locus_tag="OO309_002483"
     CDS             complement(20495..20788)
                     /locus_tag="OO309_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5847594.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20785..22074)
                     /locus_tag="OO309_002484"
     CDS             complement(20785..22074)
                     /locus_tag="OO309_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5847595.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22130..23068)
                     /locus_tag="OO309_002485"
     CDS             complement(22130..23068)
                     /locus_tag="OO309_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR5847596.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23088..23852)
                     /locus_tag="OO309_002486"
     CDS             complement(23088..23852)
                     /locus_tag="OO309_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR5847597.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTPDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24171..>24647
                     /locus_tag="OO309_002487"
     CDS             24171..>24647
                     /locus_tag="OO309_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR5847598.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDALAYTLAI"
BASE COUNT         6102 a         5947 c         6484 g         6114 t
ORIGIN      
        1 tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg cgtggtttct
       61 agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt ttctgcatgg
      121 atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt ttgtgatatt
      181 ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa cttaagtcag
      241 aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct gatgaaggtg
      301 ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt ctgataatga
      361 tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg aggatgatga
      421 ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat tgtgtcactt
      481 ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa tactaccgcc
      541 aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg ctcgcagtaa
      601 acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct ggtgtactca
      661 ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct cccttttttt
      721 agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca tttgtgtacc
      781 tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac tgttaataaa
      841 caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa ggatataaaa
      901 tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct gtaagcccca
      961 tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca gggcgcgtta
     1021 atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg tcgatcatta
     1081 ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac ctgttagggc
     1141 atgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt attgacaacg
     1201 gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc gaagcactcg
     1261 agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag aaagtcagct
     1321 ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt tccattcttg
     1381 acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa tatgtcgata
     1441 tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa ctaatgaaaa
     1501 cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt atttacggta
     1561 cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa gaagccgcga
     1621 aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag gaagaagcgc
     1681 cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg gaagtgtttg
     1741 ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc atgatggacc
     1801 gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg ttcaccacat
     1861 cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac gtacaaattt
     1921 tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag tttggtagct
     1981 gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt ggatttaccc
     2041 aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt aacggatgta
     2101 tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc gtcaaactct
     2161 ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct gaggcggacc
     2221 tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt gaaggcgctt
     2281 tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca tggcagtgcg
     2341 gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat gtgggcgaag
     2401 acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg atggagcgtt
     2461 tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt gcgccagttc
     2521 aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa ttgacgcaaa
     2581 aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag attggcttta
     2641 aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt gataaagagg
     2701 tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga agcatggacg
     2761 taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt aaacaattgg
     2821 aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac cgtatcaatg
     2881 gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag cttggtattg
     2941 tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta gtcgagatca
     3001 gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc ctctatgaaa
     3061 agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt aaggaaatta
     3121 aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc ctgattcgct
     3181 ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt gagatggcgc
     3241 accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa gaactggcag
     3301 tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg ctcgctccta
     3361 agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc ttcacaggca
     3421 ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa atgccaaaaa
     3481 ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt ggttttaagc
     3541 acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt aaacgtcacc
     3601 tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg tgcctgccgt
     3661 acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag gagagcacat
     3721 atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat tttgaaacaa
     3781 gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca ggctgttatc
     3841 aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt ccgtcaactg
     3901 tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag caaattcatc
     3961 aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga tatcgcagta
     4021 ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct ggcataagcc
     4081 agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa cattgacttt
     4141 tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta acgcaagcaa
     4201 tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag gaggctagcg
     4261 cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct ttttttgtat
     4321 gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc cagtctggac
     4381 acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga gcactgcccg
     4441 ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa cgaaccagtg
     4501 tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt gccagtgcga
     4561 aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc gtcgaatatt
     4621 tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg ccgccagaag
     4681 agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag caggcgctga
     4741 atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg gcggaaacga
     4801 ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg gttacccgta
     4861 ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca accgggccgg
     4921 aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac cacccggcgc
     4981 gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc cagacctctg
     5041 gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc gcgcctggcc
     5101 gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag atggaaggtc
     5161 tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac gacttcctgc
     5221 gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc ccgtttaccg
     5281 agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa gtgctgggct
     5341 gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa gtttactctg
     5401 gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc gtcaccgacc
     5461 tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa ggcaggaata
     5521 gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga tcgatagcga
     5581 tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag aaccggttgc
     5641 cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc atccgaacgc
     5701 tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg acatcgtctg
     5761 cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg gtgctgttct
     5821 gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg aagggatgct
     5881 gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg aactgcctgc
     5941 ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata acaccatcga
     6001 aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg cgcgtgacgt
     6061 tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg gtgcgactat
     6121 cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct atcttggccg
     6181 tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag aaaaactgcg
     6241 tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg tactgctcga
     6301 actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca ttgtggtgcg
     6361 gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga agctgaatgc
     6421 tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca tctttggtgg
     6481 cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg ctttcttcag
     6541 cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg cgtctcaccg
     6601 ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg cgacccgtct
     6661 gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca acgaagcaac
     6721 gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc tgataggcca
     6781 tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg aagtgaccga
     6841 aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg agattgaaga
     6901 agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg atgagccggt
     6961 acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca agcgcgtgaa
     7021 aacgctgctc aacgacaaag gctatcagga agtgatcacc tacagcttcg ttgatccgaa
     7081 agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc cgatctctgt
     7141 tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg tggtgtacaa
     7201 ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt tcgtaccaga
     7261 tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga tttgcggtaa
     7321 ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg atttgaaagg
     7381 cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt tccgtgcaga
     7441 agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag gtgaacgtat
     7501 tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta acggtcgcac
     7561 tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc aggcgcgcga
     7621 gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag aaaacgttcc
     7681 cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag ttggcgtaaa
     7741 cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc tcgccataag
     7801 cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg ctaccgtcgc
     7861 caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt gaacctatgg
     7921 cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt agcaagcggg
     7981 atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg gaaaacggcg
     8041 aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat caacgcccgg
     8101 gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg gtgaccttca
     8161 gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac gagtaatctg
     8221 atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag agtcaccgta
     8281 aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc ccgccaacaa
     8341 cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct ggcgcttctc
     8401 ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac tcctcgtggc
     8461 gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct ggttggtgcg
     8521 gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct ggcagaacct
     8581 ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt attgcttggg
     8641 caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc gcttatcatc
     8701 actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt attgctggct
     8761 ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta cttttccacc
     8821 tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg cgtagactgg
     8881 cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg ttgtcagtcc
     8941 aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg tttacccatg
     9001 tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt cagtgtggcg
     9061 ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg gttgtgtggt
     9121 ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag cgcattgctg
     9181 ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat tggcgtggtc
     9241 accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg acgttagccg
     9301 caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta ggagaaacga
     9361 tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg accacgctgg
     9421 agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt ggcttaacgc
     9481 cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt tttgtggtgc
     9541 tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa gagattaaaa
     9601 cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt gaagttaatg
     9661 gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc acagtcgcgc
     9721 cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg ctgtacccgg
     9781 atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa gtcatccagc
     9841 gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt aaactggcgt
     9901 tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta cccgcctggg
     9961 gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc cgaatggcgc
    10021 gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg gaagcattca
    10081 gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc atcgcgccta
    10141 tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc tgacactgca
    10201 tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc tggctcttga
    10261 tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac gcgtacgtct
    10321 tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat tgctgcttct
    10381 tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca aaattctgag
    10441 cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca accacacgtt
    10501 gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca gtggacgcag
    10561 agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt ttcgccgtct
    10621 ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt atcccgaaaa
    10681 tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc gtacggaatg
    10741 cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca ccataaagca
    10801 ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt gaacgaccag
    10861 ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg cggtgtggac
    10921 tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct gccccgtgaa
    10981 accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct gcctggtgac
    11041 ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat ttacgatacc
    11101 aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc acttgataat
    11161 gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga aaaattgacg
    11221 gcgatgatac atcgccgtca atttactcct gaatacagta gtattttgca gacaaaaatt
    11281 ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggcccc ctgaaaaagt
    11341 ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt atggcctcat
    11401 cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat gaagattttt
    11461 ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa ccagcaactc
    11521 cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggcacggt tcgtatcccg
    11581 acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt ttcagagcag
    11641 ttggaattac taaaatcatg ccaacacttt tttattcaac ataaactctt tgcctggctt
    11701 aatttaacgc cacaagtggc tacattatta ttagacaggg ataattacgc aggcgaacta
    11761 ttaaaatatc cgtttattga gctgttgatt aatgagaatt acccgcatct caatgaagga
    11821 aaagataatc gcgatctttt gtccttatcg caaatgtatc cccttgtgtt aggcaatttg
    11881 ggagcaggta atagcacaat gaaagcggtt tttgatggtc ttttcacccg cgtaatgctg
    11941 gagaaaggtt ttattcagca acaaatcacg catcgctcct tcgaaccgtt tattcgtgcg
    12001 atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat tgatacgcca
    12061 gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg cctgtggcct
    12121 gccgttccta taaatcagat aacgacgttg gttcagcgat aaccctcctg tttgctggtg
    12181 tttaagacga gagtaaccgt ctacactatc aaacagatag gatctatgac cctgtctttt
    12241 attacccgct ggcgcgatga attgccagaa acctatacag cactttcccc tacgccttta
    12301 aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag tattccatcg
    12361 tcgctgttta aaaatgccgc aggtgtctgg ggcggcgaaa ccctactgcc tggtatgtca
    12421 ccactggcac aggtttacag tggtcatcag ttcgtcgtct gggcgggcca actgggtgat
    12481 gggcgcggca ttttacttgg cgaacaactg cttgctgatg gcactacaat ggactggcat
    12541 ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc ggttttacgt
    12601 tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat tccgacgacc
    12661 cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt ggagccaggc
    12721 gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga acatttttac
    12781 tatcgccgcg agccggaaaa ggttcgtcag ttggctgact ttgccattcg tcattactgg
    12841 tcacatcttg aagacgatga ggacaaatac cgtctctggt ttaacgatgt tgtcgcacgt
    12901 accgcgtcgt taattgccca atggcagacg gtcggctttg cacatggggt gatgaatacc
    12961 gacaacatgt cgctgctggg actgacgctt gattacgggc cgtttggttt tcttgatgat
    13021 tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag ctttgataat
    13081 caacctgctg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc accatttgtt
    13141 gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt gacgcattat
    13201 ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga taacgcgcta
    13261 ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg cacattccgc
    13321 atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgagga gtttattgat
    13381 cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca agacgaggtt
    13441 agcgatagtg agcgtcagca actgatgcaa agcgttaacc cagctctggt attgcgcaac
    13501 tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgatgga attgcaccgc
    13561 ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg atgactatgt cagtcgtcca
    13621 cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact tagttaacag
    13681 cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat cgataatcag
    13741 tttgccatcc ggacctaaca gtgtctggct ggtaattcgc cggatgggct gcggggaagc
    13801 ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat aacgcataaa
    13861 aattaataat aattgatata acaatgattc ctattatcaa tattatggca gaccagagca
    13921 agcagtaatg tgttgagaag acaacaagtt gcgcagaaat gcccatccca tcaggaatgg
    13981 gcacgcattc agaagcgggt atctaccgca aaggcgagtt tttcgaccag gcgttcggta
    14041 tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg ctgaccgccg
    14101 acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc aatctccgta
    14161 gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg acggtgctgc
    14221 ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc aaactcgtgc
    14281 agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt gccgccacgc
    14341 ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg accatttttg
    14401 tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga tagcatccac ggcaatccgc
    14461 gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgctga agccatttcg
    14521 cggtggatct gactttcggt agtacgagcg ccaatcgcgc cccaactgat taaatcagca
    14581 ataaactgac cggttaccat atcgaggaac tcagtcgcgg tagggacacc cagctcattt
    14641 acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata gctgccgttt
    14701 aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt ttcaaaatag
    14761 gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg cagacgggtg
    14821 gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat gaccaacagt
    14881 cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt gacatgggtg
    14941 gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac caggctctca
    15001 atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa tttgttgctt
    15061 ctacggcagg actaccggaa agtgatgcgc atcacgataa accaatcgca gctgatttca
    15121 agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa acaaaccaat
    15181 gcactagttc tctagtacat tcggcgacta aggcccatga tatcgaggat cttggtggca
    15241 atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt acggtacaag
    15301 gcttccactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg actgttctcc
    15361 cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc gaacaattta
    15421 tgctgaagcg gtttgagcga cgcgggtagc accagattat ccatatcgtc ggcaataaag
    15481 gggtagtttg ccgcgcggat accaaattgc attgccagat acagactggt gggggtttta
    15541 ccacagcgag aaacaccgag caggatcacc tgagcctgat ccagattgcg caacgaaatg
    15601 ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata tttattgaga
    15661 ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag tttcatctct
    15721 tgttgtagcg gggcaaccag cgcctgaacg atatcctggc aaaagccttc actttgcaag
    15781 atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg cacgcctgtc
    15841 tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc attttcgaca
    15901 aacggcagcg tgatgctgct gatagtgacg ggaaattgtg acattactgc gtgtcctaat
    15961 acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc aacagcatta
    16021 tccattttgt tcttcccgtg aagcagacat aattctatga aagcataaat taaaagcgca
    16081 cagaagcgta ggacgtaatg tctggtttat aaaatgaacc ttcattttat tttttataaa
    16141 aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa attgtcgaat
    16201 agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat atttgcttga
    16261 acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca gaaatgtgtt
    16321 tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg ctcgtcaccg
    16381 ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg gggcaaaaat
    16441 gcctccctgg gtgaaatgat tactaatctt tccggaatgg gtgtttccgt tccgaatggt
    16501 ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt aaaccagcgc
    16561 atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc gaaagcgggc
    16621 gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga aaacgccata
    16681 cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc ggtacgctcc
    16741 tccgccaccg cagaagacat gccggacgct tcctttgccg gtcagcagga aaccttcctc
    16801 aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc ttctctgttt
    16861 aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg cgtggcgctc
    16921 tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt gatgttctcc
    16981 attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg gggccttggg
    17041 gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa accgacactg
    17101 gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat ccgcatggtt
    17161 tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc gcaggaacag
    17221 cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca ggccgtacaa
    17281 attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg ccacaccggc
    17341 aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca ggtcatggag
    17401 cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat cggtcatcgc
    17461 atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg catcgaacct
    17521 ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat gaagaaagca
    17581 tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat cgctcgtgaa
    17641 ctgggcattc cggcggtagt gggctgtgga gatgcaacag aacggatgaa agacggtgag
    17701 aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga gttgctggaa
    17761 tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa agtgatgatg
    17821 aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgagcga aggcgtgggc
    17881 cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg cgcactgctt
    17941 gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat gaaaggtttt
    18001 gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac gctgggtgcc
    18061 gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa cgaatatgcc
    18121 aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct cggcttccgt
    18181 ggtgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcactgga gtgtgaagca
    18241 gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat cccgttcgtg
    18301 cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg gctgaaacgt
    18361 ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc cttgctggcc
    18421 gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat gacgcagctg
    18481 gcgctcggtc tggaccgtga ctccggcgtg gtgtctgaat tgttcgatga gcgcaacgat
    18541 gcggtgaaag cactgctgtc gatggctatc cgtgccgcga agaaacaggg caaatatgtc
    18601 gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt gatggaagag
    18661 gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt aagcctggct
    18721 gaactgaaga aataaaataa atccccggcg gcgttcagtc gccggggtta tgtgatcccc
    18781 gaagatgaaa cttattcaat ctcttcacag acatcctgcg ttaatcgccg tataatatct
    18841 tttcttaaca aaaacttttg tattttatct gaggcagtgc gcggtagttt ttcgattacc
    18901 acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa agccgccatc
    18961 tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca tgatcgttca
    19021 cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg atgctgcaat
    19081 aaaatatctt ccacttcacg gctgctaata ttttcgccgc cgcggacaat aatatctttt
    19141 ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc gccgctgtaa
    19201 taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa ataccccata
    19261 aacacattgg ggccacgcga tgcttcttca ccttcgcaac ctggcggtaa ggtcttgcgt
    19321 gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt atgcataaag
    19381 cgcgacaaag aattatcgag attcaccacc gcatgcggcg aactttctgt ggaaccataa
    19441 acacttaata atttaatgct gcgctgctgg cattcgcgcg ccactttttt ggggattgtg
    19501 gtaccgccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt ctctagtaaa
    19561 ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg ctgctgctca
    19621 agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg agcgccaatt
    19681 aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt gaccaagtgg cgcaggcatc
    19741 ataaagacat ccagccaggt cagattcagc cgcgcgcaat aagcccgctc actggcgaga
    19801 atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga ggtaaacagc
    19861 accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtgggat attgtcggcg
    19921 ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac gccgacaatt
    19981 tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg acgcgtttgt
    20041 ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac ccacaccagt
    20101 tctgcttccc gccaggaagg caacagcggc acggaaaccg caccgatttt caggcaggca
    20161 agatagataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg atcgcctgat
    20221 tcaataccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc gagcgcgcta
    20281 taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat cgcacgagcg
    20341 gtctgctgcc agtaatcggc cagtgaagca tcgccccata gcccttgctg acgatacgcc
    20401 gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg aaacagaaca
    20461 tcaggcccga gttgcgggcc tgtgggatgc atgattagcc gtagcgaaaa tcaataccga
    20521 aggtgcctgc gggatattgc cactgttcga gaatagtgtt accgcacagc acccgacagg
    20581 tgccgcactc cagacaaccg gcgggatcaa aatgaatgtt tcctacgtca tcctgcttat
    20641 aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg ggattttccg
    20701 tcagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct aatttgatgt
    20761 cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc catctttcag
    20821 caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta ccggctggtt
    20881 tggtttgccg tcaatggtga acatctcgtt catgatgtcg gctaccattc gtgggtattg
    20941 gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cgaaaatgct gcatatcacg
    21001 catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgttgg cggagaaatc
    21061 cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg caatggctaa
    21121 atccatgccg cggaccgtaa aacccaaatt caggcagaag cctgcggcgt caccaacgat
    21181 catcacgccc tcgttaacca gttgcggcac cattgccaga ccgccttccg gcaccatatg
    21241 cgcggaatat tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg ggtgttgttt
    21301 aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac ccaatccaca
    21361 aaccagcccc aaggatatgg aatccttgtt ggtatagaga aatcccccgc ccatcaggcc
    21421 gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa tattaaagcg
    21481 atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag cgtaatgatg
    21541 cggatcggaa gcgggaacca ttcccagcga gcggccaagc atcgagttaa cgccatcagc
    21601 cagaatcacc acattcgctt cgagaatatc atccccagcc tgcacgccag tgaccttgtt
    21661 tccttcacga accagcgcgt cgacgcgaac gcctgggata aactgcgcgc ccgcctgctc
    21721 ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag atgcgtgttg
    21781 aggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt cggttaagaa
    21841 ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa agcctggaat
    21901 gattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac atccggcact
    21961 gtcgccgcgt tctatcacca ggacatccag cccggctcgt gccatgacca gtgcggcaac
    22021 gctaccagca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg acatgcgtta
    22081 tgctcccttc ctgctgcgtt tacagcggga gatgtggttg aaaggggaat tagcggctca
    22141 actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg tagtcggcat
    22201 agttgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt acgccgttgc
    22261 cgccaaccat atgttggatc tgcccggaga tccccagcgt caggtagaga tcggatttca
    22321 gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg ccttcggcaa
    22381 ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg accattttca
    22441 ggtcatcctg cgccgccaga ccacgaccga cgccaaccac acgttttgct ttgctcaggt
    22501 ccacgctgct tgcggctttc gcacggcgtt cgcgacagag aatttcatga cgcggagcaa
    22561 cataaggtac cgtttctgtc gggcactgat gagaggtatc actggtgcac ggttcctgaa
    22621 caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctga gcgaacgcta
    22681 acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc accgccgtgg
    22741 catcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga gctttaccac
    22801 gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc tttcagcagg gcagcaatgc
    22861 tttcggcgta attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca taaatacatt
    22921 ttggaccata aggcataact gcctgcgcct ggtcggtatt ttgtacaatg gcatacacct
    22981 gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga ttatcgctaa
    23041 agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca gttcagggct
    23101 ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc gagaatgatg
    23161 tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca gttcagcaag tggcgcgctc
    23221 tggctccagt caatatcact tgcctgccac tgatttaccg gttttttacc cgcgccgaga
    23281 atggctttca tcgaaggaat gcgtggcacg ttaatatcgg gggtgacgca gagcacggct
    23341 ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat caccagtgta
    23401 ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag aatttctccg
    23461 accagcaagc caacctgctg ggcataaagg tcgccggaac cttcaccaaa gatcagtaaa
    23521 tcgaagccga tcttttcaat tgctgccgcc agcgcttttg cggtatcgag aggcagtgca
    23581 tgttcaagtt gcgcatcctg caccaaatac aggctgtgcg gcccgcggga tagcacgtct
    23641 ttgcgcactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc tatctcatcg
    23701 tcatctgttg cgagctggct tgcagcttca atggcattga gatcgaactg gctgattttg
    23761 gcgtcggcat tgtcgaaatt cagggtgtat tctggagtga caacaatgtc ctgttcttca
    23821 ggcaccagct taaagcaggt tattattttc atgatttctc ctgtgagtcg catacggaac
    23881 catctccgat ggaaggtaaa aatggcctga aaatagtgtg cgatagcggt gcgggggggg
    23941 gggcgacgga gcaagctgtt ttccttgcca acaatcttgc aaaaaaaatg cgattgctaa
    24001 aacaaaagtg caatttgcgg caatatagtt attccatgac cgtacaatct tgcattcagt
    24061 gtaaataatc ttgtttagct ctcgctaaac gcggttgtat aaccgcaata cacgccgtat
    24121 tttatccggc atatattgtt tttgcttgct catggaatag ggaggttgtc atgtatcaac
    24181 gctgttttga taatgccagt gaaacgctgt ttgtcgccgg taaaacgcca cggctttcac
    24241 gttttgcatt tagcgatgat ccaaaatggg agtctggaca tcacgttcat gacaatgaaa
    24301 ccgagctgat ttacgtcaag aaaggggttg caaggtttac catcgattct tcgttatatg
    24361 tcgcacatgc ggatgacatt gtggtgatag aacgcggcag gctgcatgcg gtggcctctg
    24421 acgttaacga tccggcaacg acgtgtacct gtgcgctgta cggctttcag tttcaggggg
    24481 ctgaggaaaa tcagttactg caaccgcatt cttgtccggt aattgccgcg gggcagggaa
    24541 aagaagtcat aaaaacctta tttaatgagc taagtgtgat tttgccgcaa agtaaaaata
    24601 gccaaacatc ttcgttatgg gatgcattag cctatacatt agcaatt
//
LOCUS       DAJTQQ010000057        24128 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000057 DAJTQQ010000000
VERSION     DAJTQQ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.057"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..539)
                     /locus_tag="OO309_002488"
     CDS             complement(<1..539)
                     /locus_tag="OO309_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR5847599.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNP"
     gene            757..1617
                     /gene="tesB"
                     /locus_tag="OO309_002489"
     CDS             757..1617
                     /gene="tesB"
                     /locus_tag="OO309_002489"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR5847600.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1666..2952)
                     /gene="amtB"
                     /locus_tag="OO309_002490"
     CDS             complement(1666..2952)
                     /gene="amtB"
                     /locus_tag="OO309_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR5847601.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(2982..3320)
                     /gene="glnK"
                     /locus_tag="OO309_002491"
     CDS             complement(2982..3320)
                     /gene="glnK"
                     /locus_tag="OO309_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR5847602.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3501..5282)
                     /locus_tag="OO309_002492"
     CDS             complement(3501..5282)
                     /locus_tag="OO309_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5847603.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5275..7047)
                     /locus_tag="OO309_002493"
     CDS             complement(5275..7047)
                     /locus_tag="OO309_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5847604.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7077..7535)
                     /gene="decR"
                     /locus_tag="OO309_002494"
     CDS             complement(7077..7535)
                     /gene="decR"
                     /locus_tag="OO309_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR5847605.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7689..8507)
                     /gene="cof"
                     /locus_tag="OO309_002495"
     CDS             complement(7689..8507)
                     /gene="cof"
                     /locus_tag="OO309_002495"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR5847606.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8607..10299
                     /locus_tag="OO309_002496"
                     /pseudo
     CDS             8607..10299
                     /locus_tag="OO309_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10364..11059
                     /gene="queC"
                     /locus_tag="OO309_002497"
     CDS             10364..11059
                     /gene="queC"
                     /locus_tag="OO309_002497"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR5847607.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11111..11509)
                     /gene="fadM"
                     /locus_tag="OO309_002498"
     CDS             complement(11111..11509)
                     /gene="fadM"
                     /locus_tag="OO309_002498"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR5847608.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11603..11974)
                     /locus_tag="OO309_002499"
     CDS             complement(11603..11974)
                     /locus_tag="OO309_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR5847609.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12125..13996)
                     /gene="ppiD"
                     /locus_tag="OO309_002500"
     CDS             complement(12125..13996)
                     /gene="ppiD"
                     /locus_tag="OO309_002500"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5847610.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14188..14460)
                     /gene="hupB"
                     /locus_tag="OO309_002501"
     CDS             complement(14188..14460)
                     /gene="hupB"
                     /locus_tag="OO309_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR5847611.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14669..17023)
                     /gene="lon"
                     /locus_tag="OO309_002502"
     CDS             complement(14669..17023)
                     /gene="lon"
                     /locus_tag="OO309_002502"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR5847612.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17211..18485)
                     /gene="clpX"
                     /locus_tag="OO309_002503"
     CDS             complement(17211..18485)
                     /gene="clpX"
                     /locus_tag="OO309_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR5847613.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18611..19234)
                     /gene="clpP"
                     /locus_tag="OO309_002504"
     CDS             complement(18611..19234)
                     /gene="clpP"
                     /locus_tag="OO309_002504"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR5847614.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19481..20779)
                     /gene="tig"
                     /locus_tag="OO309_002505"
     CDS             complement(19481..20779)
                     /gene="tig"
                     /locus_tag="OO309_002505"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR5847615.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21123..21440)
                     /gene="bolA"
                     /locus_tag="OO309_002506"
     CDS             complement(21123..21440)
                     /gene="bolA"
                     /locus_tag="OO309_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR5847616.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21745..22323
                     /locus_tag="OO309_002507"
     CDS             21745..22323
                     /locus_tag="OO309_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5847617.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22367..23842
                     /gene="ampG"
                     /locus_tag="OO309_002508"
     CDS             22367..23842
                     /gene="ampG"
                     /locus_tag="OO309_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR5847618.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5653 a         6654 c         5806 g         6015 t
ORIGIN      
        1 ggattcacct gagttggtga agtcgaaggt acggtagtcg ttgcgccacc gctggcctga
       61 accggcacgg cggtttgctg taccggcacc aatgtcaggt cggctttagt tccgccctgg
      121 ttgatcaccg gctgaacggt atcggtgata aataccagtt tgtcattcac ggtaatcgcc
      181 gcactcaaca gaatacgcgc gttcggctga acatctgccg ggttaaatgg cagaacaaag
      241 ctgaatggtg actgtttacc ttcagtacgc accgctttct gcgccagcac ttttgacggt
      301 gcatcggcta acgacgcgtc agaaagtgtc acggtcagca cagcatcagg cggcagtgcg
      361 actttctgac ggatccagac agtaccggag acattcggtt gctggatagc aggttgttgt
      421 gttgctgaaa tagacgtatt tgcagccggg gctggcgtct gaatatccgc gcttttatct
      481 gcgcaagccg ccaacgcaat cgcaaccgct aaaccactgg ccatgtgcac gagtttcatt
      541 catttctcct tattatcaat gcaccagcgg gctaactttc ctcgccggaa gagtggttaa
      601 caaaatagta acgtcaacaa gtgtggcaca catcacgcat ttctgcctgt aattagcccg
      661 taattcagac tatttcaccc atcggaccag tggcaaattt gcgttatact caactcactt
      721 tggcttgctg cggcagcttt gttactggag agttatatga gtcaggcgct aaaaaattta
      781 ctgacattgt taaatctgga aaaaattgag gaaggactct ttcgcggcca gagtgaagat
      841 ttaggtttac gccaggtgtt tggcggccag gtcgtgggtc aggccttgta tgctgcaaaa
      901 gagaccgtcc cggaagagcg gctggtacat tcgtttcaca gctactttct tcgcccaggc
      961 gatagtaaga aaccgattat ttatgatgtc gaaacgctgc gtgacggtaa cagcttcagc
     1021 gcccgccggg ttgctgctat tcaaaacggc aaaccgattt tttatatgac ggcctctttc
     1081 caggcaccag aagcgggttt cgaacatcaa aaaacaatgc cgtccgcgcc agcgcctgat
     1141 ggcctgcctt cggaaacgca aatcgcccaa tcgctggcgc acctgctgcc gccagtgctt
     1201 aaagataaat tcatctgcga tcgtccgctg gaagtccgtc cggtggagtt tcataaccca
     1261 ctgaaaggtc acgtcgcaga accacatcgt caggtgtgga tccgcgcaaa tggtagcgtg
     1321 ccggatgacc tgcgcgttca tcagtatctg ctcggttacg cttctgatct taacttcctg
     1381 ccggtagctc tacagccgca ctgcatcggt tttctcgaac cggggattca gattgccacc
     1441 attgaccatt ccatgtggtt ccatcgcccg tttaatttga atgaatggct gctgtatagc
     1501 gtggagagca cctcggcgtc cagcgcacgc ggctttgtgc gcggtgagtt ttatacccaa
     1561 gacggcgtgc tggttgcctc gaccgttcag gaaggggtaa tgcgtaatca caattaaaaa
     1621 aatagccgga ggtgaaaacc gtccggctat tttttgcagt gcttgttacg cgttataggc
     1681 attctcgccg tggctgttga cgtccagccc ttctcgctct tgttcttccg gcacacgtag
     1741 gccaaccgtc agatccgcca gtttgtagcc aataaatgcc accacaccgg accagacgat
     1801 cgtaatggcg atgctttcca actgtaccag caactgatgg cccatcgtca caccttcagc
     1861 gaagcccacg ccgcccagag aactggcggc aaaaatcccg gtcatgatac agccgacaat
     1921 gccacaaacg ccgtgcacac cgaagacatc gcagggatca tccacccgca gcaagcgttt
     1981 gagcatggta acgccccaca aacccgccag accagctacc acgccgataa tcaacgcgcc
     2041 gccaacccca atgtagccgc acgctggcgt cacgccgacc agaccggcaa tcgcgccaga
     2101 acacgctccc agcagagaag gcttaccacg cagcgcccat tcaccgaaga tccagccaag
     2161 aattgccgcc gccgttgcga ccacagtatt cacaaatgcc agtgccgcga tttcattcgc
     2221 cgtgcccact gacccggcgt taaagccaaa ccaaccgata tagagaatgg cagtcccggt
     2281 gaagaccatc ggcaggttgt gcggtttaaa cgcctcttta ccgaagccta cgcgtttgcc
     2341 tatcagatac gcgcccacca gaccggcgat tgctgcgtta atgtgcacca ccgtgccacc
     2401 cgcgaaatcc agcgcaccgt gagaagccag caaaccaccg ccccacacca tatgcgcaat
     2461 cggaatgtaa gagagcgtca gccataccac cacgaaaatc aacacagctg agaagcggat
     2521 tcgttccgcc agcgccccaa ctatcaagcc tacggtaatg caggcaaacg atccctgaaa
     2581 cgctacgtgg atatactgat aaatgctgcc catcaccgcc gtcagttcga tgttttttag
     2641 catcaaccag ttaatgttgc cgaagaagtt gttgccctca ccaaacgcca gcgaataacc
     2701 gtaaaccacc cagagaatac agaccagtgc aaacgtcacc gtcacctgcg tcagcatcga
     2761 cagcacgttt ttgccgcgaa tcaacccacc gtaaaacagg gcaatccccg gaatagtcat
     2821 aaacagcacc agcgcagtac aaatcatcat aaacgcattg tcggctttat cggccaccgc
     2881 aggtgcagcc attaccagtc ccggcagcat cgccagtgaa gcaagcccag tttttatcgt
     2941 cgctatcttc atttttcgtt cctgttgctg tgtgccagag attacagcgc cgcttcgtcg
     3001 gcttcgccgg tacgaatacg aatgacgcgt tgcaattcag cgacgaagat tttgccgtcg
     3061 ccaatttttc cggtgtaagc cgccttactg acgatatcga tcacttcatc gagttggtca
     3121 tcagcaatcg ccacatcaat ttttactttt ggcaggaagt tgacgctgta ttccgccccc
     3181 cggtacagct cggcatgccc tttctgacgc ccgaaacctt tcacttcggt gacggtcagg
     3241 ccctgaatac caatggaaga taacgcttca cgaacgtctt ccagcttgaa tggtttgatt
     3301 atcacggtca ccagcttcat agatcccctc cggtcagaat tcggtaatgg ttctgctaca
     3361 cgaagaaggt attgcaagcg gtgtgccaga aatgaaaaac gagaaagaga gcaggaagtt
     3421 aagaagattg aaaatgaaaa cgcactatga cagtgcacag tgcgttacat ttttgcacca
     3481 acgatgaaat attgcgctat tcaggcgctc aatgattcct cttcacgcac gctggctgcc
     3541 agctcttcgc ccgcaagttg cagttgatac atctgccagt agcgtccctg ggccgccagc
     3601 agttgctggt gagtgccctg ctccacggct tgcccacgat gaagcaccag aatggtgtcg
     3661 gcatcaataa tagttgataa gcggtgagca atcaccacca gcgtggtatg ttcacgcacc
     3721 gccgccagag catgttgaat cgcctgttca gtaccggagt caatgctggc ggttgcctca
     3781 tcaaggatca ggatttgcgg cgtctccacc agcacgcgcg ccagtgccag cagttgcttt
     3841 tgcccgactg agagattatt tccctgctcg cccagcggcg tataaataca atcactcatg
     3901 ctgcgcgcca gctccgccag ttgcacggtt tccagcgcct gccagacgcg ttcttcggag
     3961 atatcccgcc ccagcgtcac attagcgagg aaggtatccg ccagcaccac cggatcttgc
     4021 tgcaccattg ccacgccctg gcgcagcgcg ctatgactta gcgaacttaa tggacgacca
     4081 tcaaggtgaa tctcaccttc cgttagcggg taatagccca tcaataaact ggcgagggta
     4141 cttttaccac tgccggtatg cccgaccagc gccacaaagt tacgcgaagg cacagagaga
     4201 ttaatgttct ttagcaccag attgtcatcg cgataagcaa atgacacgtt atcgacttcg
     4261 atggtgccac tctgtaacgg gtaatcatca ttgccatatt gctggcgcgg tccgtccatc
     4321 agttcaaaca cgcgctcacc agcaacaaca gcctgttgca gcatcgcctg ttgcgtggtc
     4381 agttcgatta agggttcgtt aagtcgccca agatagctga taaacgcata caacacgccc
     4441 acttcaatgg tgccgctggc ggagaagcca aacagcatca ataagccaca aagaatgagc
     4501 gatgaaaaca gactcagcag cggacgcagc agaaaaccgt cgaggcgcag ggtttgcatc
     4561 ctagccatat agtgtgaacg actggcctcc cccatacgtt cgccaaatcg cgcctgctga
     4621 cgaaactgct ggataacgct catgccattg atgatttcgt taaagccgtc gttgatatcc
     4681 gccagatagg cgcgcacacg gcggacaatc ggcgtgctgt aacgctggta tatcaccatt
     4741 accaccagca ccaccgggaa aatcattatc gccaccagcg ccattcgcca gtcgaggctg
     4801 aacatcgcca ccagcatcgc gcccaccagt gcggcactgc gcaggacagt tgccactacg
     4861 gtaacgtaga gatcgcggat cacttcagtg tcattagtga cgcgggaaat cacctgcccg
     4921 acgggttggg tatcaaactc gcttaatggc tggcgtaacg cagcatccat cacgtcggta
     4981 cgcaactgtt gcactgcgcc tactgccgcc cgattaaaca gcagcgactg cgcgtaatgt
     5041 agcccggcgg caaacagttg tagcccaaca tacgccgcag ccagccctgc aaccactttc
     5101 aacggcaggt tatttttcgc taccatattg tcgataaaat agctgataag cagcggccca
     5161 ctgacttctg ccgccgccgc aacccacatc atcaggaccg caatccccag cggtttacgc
     5221 cacggcgaac catacgctaa caggcgcttg agagtcggcc acagttggct aaaactacgc
     5281 atcgacggcc tcctcgcgaa tttccggaac gtcgtcgagc gccgcctcca gttgttgata
     5341 gcgatacata tcgcgatacc aaccgctttg ttgcgccagc tcatcatgat tgccacgctg
     5401 ggcgatatgt ccgtgctgca tcacaataat ttcactggct tccgtcagtg ccgaaaggcg
     5461 atgggcactg atgattaccg ttcttccctg tccccactga cgcaggttat gcaggatctg
     5521 gtgctctgtg cgtccgtcca ccgccgaaag cgcatcatca aggatgagga tttccgcgtt
     5581 gactaataac gctcgagcaa tggagatacg ctgtttttgc ccaccggaaa gcatcacgcc
     5641 gcgctcgccc acttctgtat cgtaaccttg cggtagacgc aaaatatcgt catgtacgct
     5701 ggctaaccgc gcgacatgct caatctcttg ctgggtggca ttcgggcaac ccagcgcgat
     5761 gttattcgcc acggtgtcag aaaaaaggaa tggcgtctgg ctgaccaccg ccaggcggct
     5821 acgccagcta tcgagttgta acttcgtcag aggaatatca tgaaagcgaa tatccccctc
     5881 gctgacgtcg aaatgacgct gaatgagcga caacagggta cttttgccgg aaccagtcgg
     5941 cccgcaaata cccagcatct gaccgggttt cagggcgaaa ttgacgtttt ccagcgcagg
     6001 atggtcagtc tgcggatacg tgaactggtg aatatttaca tccagttcgc cacgcccttc
     6061 cggcacgggt tcgctaccat cgttcaccac cggcgcttcc gccagcatgg cgcgaatacg
     6121 gctgtatgca gcactaccac gttccacaat gttaaacatc catgccagcg ccagcattgg
     6181 ccaaatcatc agacctaaat acatcataaa actggtgagc tggcccagcg ttaaactgcc
     6241 ctgcaccacc atccaactac cgccgccaat cgccagcaag ttcgccatac caatcgcgat
     6301 atagatggtc gggtcgaagc gagcatcaat acgcgccacc cgcatgtttt ttttgccggt
     6361 atcttcggca tccgcggcaa ataacgccga ctggcgatct tccagaccaa aggctttgat
     6421 catgcggata ctggtgaggc tttcctgggt gcggtcatta agactggaaa acgccgcctg
     6481 tgccagctta aagcgttcat gcaaggcatc gccgttgcgc ttaatcatga tcgccatcac
     6541 tggcatcggt aacagggcaa ataaggtcaa ctgccagcta atttgcgtag acatcataat
     6601 caacacagcg cagcccatta ccagtgaatc caccagcgtc agcacccctt ctccggcggc
     6661 aaacacgacg cgatcgacgt cattggtcgc acgagccatg agatcacccg tgcgatgacg
     6721 caggtaaaac tccgggtgct gccggcttag ctgacggtaa taatcttcac gcagttcaac
     6781 agccagttgg taagacgcgc caaacagcaa tacgcgccag acataacgca ggagataaac
     6841 cacaacggca atcagcacca tggtggcgat ccacatcagg ctcagcccgg tagtaaagtg
     6901 ttgttctgtc acgccatcga caacaatacc aaccactttt ggcggaacca gttgcagcat
     6961 cgcgataatg acaagcaagg cgacagcccc aagatagcga cgccattccc gacggaaata
     7021 ccagcttaat tgagcaaata atcgcacgcg ttgtgtcctg acctgattct ggatatttat
     7081 tcgatgggta aagaagtggt gtatttaatc tgttccatcg cgaagctgga agtgacgtcc
     7141 gacagccccg gcacgctgtt taccagacgc ttataaaact cgtcgtagcg tttcatgtcg
     7201 gcaacctgga cgcgcatcag ataatcgtat tcaccagcca tgcgccagac ccccagcact
     7261 tctggcattt cggtaaccac cgtgacaaag cggcaatacc attcgctgct gtgatgttgc
     7321 gtttttatca gcacaaaggc ggtcaggccg aggcctattt tttccggatc cagcagggcg
     7381 actttgccga taaggatacc gtcgtcctcc agccgtttca ggcgcttcca gcagggggtg
     7441 gttgtcagat taacggcttc agccagtgcc tgcaaagaga gggtgcaatc ctgctgcagt
     7501 aaggccagca gcttacggtc aattttatct aacatagccc ttccacggag aatttttttc
     7561 tcttttaatt ctattttaaa ggtgaaatcg cgacaattta ttctgtgcgg tttaaacacc
     7621 acggcacaaa atgacaaatt caacgcaaaa aaaaaccggg caattgcccg gtgctggaag
     7681 ggatctcgtt attcggggga ataaggtaga tgtggatagt ccagccagtg cgtcaaatag
     7741 tgagagacag cctgatttcg gcaatgtcca atcaccggta aatgcgggag ctccgcgcgc
     7801 agttgcggca tcgcattgcc cataataaac ccgctaccga cgctgcctaa catttcgcga
     7861 tcgttcatcg catcaccaaa ggccatgcaa tcgcgcaacg ataaacctaa atgttgggtc
     7921 cgcaccgtca atgcagcgcc tttattgcag cccaccggca gcacttcgag gcaatccgtg
     7981 gcggaaaaac acaaatgtgc acgctcgcct aatgcttcgt atagctggat ctgcaagcgt
     8041 gtaagatcgt cgtgatcgcc acagaagcag atcttggtga cgctgccgag tggcattttt
     8101 ttgacatcga ttatctgata acgaaaaccg ctatagacaa atacctgcaa caacgcaggg
     8161 ctctctttcc cggtaaacca accgtcgtca ttgaagatat gcatgctggc tcgggtatcc
     8221 cattgctgat acagcaccag ctccgcgaca tccgcaggta aatcatcacg atgtaaaagt
     8281 tcaccttcca gagaatgcac gcgcgttccg ttgccggtaa tcaaatatgc atccagcgat
     8341 agcgccccga gaatatgctg catctccagc gcatgacgcc ccgtggcaaa agtgagggta
     8401 atgtcgcgtt cacgcagtcg cgccaaagta gagagggttt tctcacctaa atgatggtca
     8461 ggcatcaata aagtgccatc catatcaaat gctaccagac gagccatttc tttctccact
     8521 ctgtgacacg gttaatggtg attgagtatc acctgatata tacggaagta atagtgaata
     8581 gttaaataat attgttccgg gtttatatgc gattgctcaa ccgtcttaac cagtatcaac
     8641 gtctgtggca accttcagcc gcaaaccgtc accgtcagcg aactggccga acgctgtttt
     8701 tgcagcgaac gccatgttcg tacgctgttg cgtcaggcac aggaggcggg atggctggag
     8761 tggcaggcgc agtcaggacg cggaaagcgc ggacaattac gctttctggt cacgccggaa
     8821 tcgctacgca atgcgatgat ggaacaggca ctggaaaccg gaaagcagca agatgtgctg
     8881 gagctggcgc aactggcccc aggtgagctg cgcactctgt tacagccatt tatgggcgga
     8941 caatggcaaa acgatacacc cacgttgcgt attccctact atcgcccgct cgaaccgcta
     9001 caaccaggct ttttgcccgg ccgtgccgag cagcatctcg ccgggcagat attttccggc
     9061 ctgacccgct tcgataataa tactcagcgc ccgattggcg atttagcgca tcactgggaa
     9121 acctctactg acaggttacg ctgggacttt tatcttcgtt caaccctaca ctggcataac
     9181 ggcgatgcag taaaagcctc acacttacac cagcgattat tgatgctgtt acaactgcca
     9241 gcactggatc aattatttat tagcgtgaag cgtattgaag tcacccatcc gcagtgtctg
     9301 accttctttt tacatcgccc cgattactgg cttgcgcacc ggctggcgag ctattgcagc
     9361 catctggcgc atccgcaatt cccactgatc ggcactggtc cttttcgctt aacacaattc
     9421 acagcggagc tggtgcgcct ggagagccat gattattacc atttgcgtca tccgctgctt
     9481 aaagcggttg agtactggat aactccgccg cttttcgaaa aagatttggg aaccagttgt
     9541 cggcatcccg tgcaaatcac catcggcaaa ccggaggagc tgcaacgggt cagccaagtc
     9601 agtagtggca tcagtttagg tttttgctat ttaacgttgc gcaaaagtcc ccgactctcc
     9661 ctctggcagg cgcgaaaagt gatctccatt attcatcaat ccggtttatt acaaacgtta
     9721 gaagtcggag aaaacctgat caccgccagt catgcattac tgccaggctg gactattccg
     9781 cactggcaag taccggatga agtcaaacta ccgaaaacct tgacgctggt ttatcaccta
     9841 ccgatagaac ttcataccat ggcagaacgc ctacaggcga cactggcagc ggaaggctgt
     9901 gaactcacaa ttatttttca taacgcaaaa aactgggacg acacgaccct actggcacac
     9961 gcagacctca tgatgggcga cagattaatt ggcgaagcac cggaatatac tctggagcaa
    10021 tggctacgtt gcgatccact atggccacat gttttcgacg ctccagcata tgcacatctg
    10081 caatcgacgc tggacgcggt gcaagtaatg cctgatgagg aaaaccgatt taatgccctg
    10141 aaagcggttt ttagccagtt aatggcagac gcgacgctga cgccgctgtt caactatcac
    10201 tatcgcatta gtgcccctcc cggcgtgaac ggtgtgcgac tgacaccgcg cggctggttt
    10261 gaatttaccg aagcctggct tcccgcgccg tcgcaatgaa tagctggtcc gggttaatcg
    10321 caggcgttac cataacgctt ttattgttca agcaggatta tctatgaaac gtgctgtcgt
    10381 tgtgttcagt ggaggccagg attccaccac ctgtctggtg caggcattac aacaatatga
    10441 tgaagtccat tgcgtgacgt tcgattacgg tcagcgacat cgcgcagaaa tcgacgtggc
    10501 acgcgaactg gcgctgaaac tgggagcacg cgcgcataag gttcttgatg taacgctgct
    10561 caacgagctg gcggtcagca gcctgacacg tgacagcatt ccggtgcctg attatgaacc
    10621 tgaagccgat ggcatcccga atacgtttgt cccagggcgt aatattttgt tcctgacgct
    10681 ggcggcaata tatgcgtatc aggtaaaagc agaagccgta attaccggcg tctgtgaaac
    10741 ggatttctcc ggctacccgg attgccgcga tgagtttgtg aaagcactaa accatgccgt
    10801 cagtttgggc atggcgaaag atattcgttt tgaaacgccg ctgatgtgga ttgataaagc
    10861 ggaaacctgg gcgctggcag attattacgg caaactggat ttagtccgta acgaaacgtt
    10921 gacctgctat aacggcatta aaggcgacgg ttgcggtcat tgtgcggcat gtaatttacg
    10981 cgccaacggt ttgaatcatt atctggccga taaaccgacg gtgatggcag cgatgaagca
    11041 gaaaaccggg ttgaggtaat tattccgggt gtcgccggat gcggcttgag catccggcac
    11101 cacaaaacgt ttacttaacc atctgctcca gcttttcgcg caattcccct tccagagcta
    11161 atgctttctg cgttttaaga tcaatacaaa caaacgtaat aagcgcatcc gctaccacct
    11221 gcccttccgg ctccagtgta atgacctggt ttaagatgcc gcttttaccg tttaattgct
    11281 gcaactgact ggtaatagtt aacaggtcgc ttaataccgc tggacgacga tagttaatat
    11341 tgatattgac cacgacgaag gcgatgttat gggccgtcat ccactgaaaa ctgtcgctat
    11401 tttccacccc atcccagcgg gcttcttcga gaaattcaag gtagcgggcg ttgttgacgt
    11461 gctggtaaac gtcgagatga tatccacgaa ctttgatttg tgtttgcata gcgcaataac
    11521 cttacgttgt tgttataagt acagaggtca taactggtat gacctcttca gtctggcaaa
    11581 aaattgccac tatgcaaatt aattacaggg ttaataccgc cagattacgt tccaccagcg
    11641 aattgcccat ccccggcacc tgctttagat cctccaccgt tttaaacgga ccgtactctt
    11701 cgcgataact gacaatcgcc tgcgctttct tcaggccaac gccattcatc gcgcgggcta
    11761 gctcttccgc gctggcatta ttaatgctga cccgggtgcc ttcttcgtca ctggctttcg
    11821 ccggtactgc tgctttactt tgtgctgcag gagcttccgc tttggtttct accgccgtcg
    11881 gtttcgccac agaagctgcc gccagcgcgc tatgagacat tccggcacag gccagggaca
    11941 gtgtaatgag cagtgcttta attccgtgtt tcatactgtt ttctccttgt ttgttaacag
    12001 tggaatcacc ttaacgggag tgaaaacagt aaacaaatgg caatgttcag aaatggaaaa
    12061 ggccgcgaaa gcgacctttt cttgttacag ctgcaacgca tttttttgcg aggcggcttc
    12121 aggattattg ctgttccagc gcatcgccaa ttttgatttt cgcctcttta cgcaggttac
    12181 tcatcagagc ttcaaagacg atttgtgcgt tgttctgggt gataccctgc accatcgctt
    12241 ttttctgatc ttccggcatt gaaccttgtt tcacttcatc cagcgccagc agaaccacat
    12301 taccttgcat atcggtcgcc ataccgtagc tcggtttgtc tttcgctggc agtggcagtg
    12361 caaacgccgc ctggctaatc gggtcacgac cggaacggct taaggttttc ggctcgccaa
    12421 atttcagacc ggcagcctgc atagcttccg cacctttgcc ggctttcaaa tcaaccagca
    12481 gtttctcagc atccactttc gcctgttgtt cagctttgtt gtgctgaacc agtgccttaa
    12541 cttgttcctg aacatctgcc aacggtttca ccgcttccgg tttgtgctcg ctgacgcgca
    12601 gcacgaatgc gcggtcgccg tctacggtga tgatgtcaga gttgatgccc gacgcgccgt
    12661 tttcacctac cagaccgccg ttaaagatag cgtcggcaac cggcttgaag ttcaactctt
    12721 ccggcaggtt atctttgctg aaccaacccg tctgagtggc tttaacgccg gcagcttgct
    12781 ctgcaccggc cagagactcg gtgtcgttgc ttgccgcatc gctcactttc tgctgcagcg
    12841 catagtacgc atcgagggct ttttcgtgtt tcactttcgc cgcaatgtcg tcacgtactt
    12901 cgtctaacga tttcactttc gctggctgaa tgtcgtccag acgtacaatc aggaaaccga
    12961 ccgaagattt gatgacacca gacagttggc ctttttcttt cagaccagca tttttcagtt
    13021 cgtcggggat agtggcatct tctaaccaac ccatatcgcc gccgttacgg gcagagataa
    13081 tatcggcaga tttttctttg gctaatgcag caaaatcacc gcctttattc agctcatcaa
    13141 gtaccgcttt cgcttcatct tcagttttgg tctggatgat gctgtagcgg gtacgctgcg
    13201 gctgggtgaa ttgatcctga tgctgatcgt agtagctctg gatatccgca tcgctaaccg
    13261 gttgctgcat cgttgcggca accagcttga tgtaactcac gcggaattgt tccggcgtca
    13321 tgaaattgtt tttgttttgt tcgtagtagc tggcaatctc ctgttcggtc acaggctgct
    13381 tcgccgccag cgcgttaaca tcgatagtcg cctcacgcac cacgcgttgt tgcgcgacca
    13441 gtgccgccag ctcgtcggtt tcacctttca gcataaaatc ggtaccggca acgccgttaa
    13501 tcagctgttg ggtagtgagc tggttacgca gcgcctgggc gtactgatcg gcggtcatcc
    13561 ccatctggtt gaggataccg ttatagcggc tgttatcaaa tttgccgtca acctggaagg
    13621 ctggggtcgc gaaaatcgcc tgtttaacct gctcatcact gatacccagt ttcagctcac
    13681 gagcgtactg atccaacagc gcctcgtcga tcagacgatt cagcacctgt tgacgcaggg
    13741 ttttcatata gccttcgttc gctgccagct cggagtattg atcgcccagc tgttgctgca
    13801 tacgattacg ctcgctgtta aaggcgttct cgaactgccc acggctgatt tcctggtcat
    13861 tcacttttgc ggcgtagtta ttgcctccgc caatcaggta accactcacg ccggtcaata
    13921 tgaacgacac gataatgata ccgaaaataa tcttgagcac gagactgttt gcagccgtgc
    13981 gtaagctgtc catcatggtg taacaacact ccgctgtaga tgactgtgta cctcacgcaa
    14041 cgtatcgatg cgcgcaaagg cctattgtga caagaaacgg gggcaattgt cagcccacaa
    14101 gacacagaaa gtcaccgaat acaaataaaa aaggcacatc agtagatgcg cccttgaact
    14161 tcgtcacatc cccactgggg acaacgctta gtttaccgcg tctttcagtg ctttacctgc
    14221 acggaagctc ggtactttag cggcagcgat ggtgatctct ttaccggtct gcgggttgcg
    14281 gccagtacgg gcagcacgct ctttaacggc aaaagtacca aaacctacca gtgctacatc
    14341 atccccttct ttcagagatt cagttacgga agcaataata gcatctaacg cacggccagc
    14401 cgcagcttta gagatatcag cccctgcagc aatcttgtcg atcaattgag atttattcac
    14461 tcttctcttc ctctttataa tttatatcgc acttgaatcc ttcaaggtac gaacgcgcag
    14521 cagttatatc aggccagcca tccccttaca acacccctta ataagggcaa gcccgatccg
    14581 ctatctaact tagcgagaca aaaaaaggct ggcaagcccg aattagcctg ccagccctgt
    14641 ttttattagt gcattttgcg cgaggtcact attttgcagt cacaacctgc atgccagacg
    14701 gttcattttg cagcgccaga gtcagaactt cctcaatgcg cttcacagga tgaatgtcca
    14761 gatcggcaat tacgttgtca ggaatctctt ccagatcgcg tttattttcg aacggaatta
    14821 gcactgtttt aatcccgccg cgatgcgctg ccaggagttt ttctttcaaa ccaccgatcg
    14881 gcagtacctg accacgcaga gtgatctcac cggtcattgc cacatcggca cgaaccgggt
    14941 taccggtcag gcaagaaacc agcgcggtgc acatagcaat accggcactc ggaccatctt
    15001 tcggcgtcgc accttccggt acgtggacgt ggatgtcacg tttttcgtaa aaatcagggt
    15061 tgatccccag tttttccgca cgcgcacgaa ccaccgttaa cgccgcctga atagactcct
    15121 gcatcacttc gccgagcgaa ccggtatagg tcagtttgcc tttacccgga acacatgcgg
    15181 tttcaatggt cagcaagtca ccgcccactt ccgtccacgc cagaccgatt acctgaccga
    15241 cacggttttc gttatcagcg cgaccatagt cgaaacgctg aacaccgagg tagtcatgca
    15301 ggttatcgcc gttaatttcg atatgtttta atgacttatc gagcagtaac tgcttaaccg
    15361 ctttgcggca cagtttggag atttcacgct ccagaccacg cacgcccgcc tcacgggtgt
    15421 agtaacgaat aatgccgata atggcgctat cgtcgacggt cagctcacct tttttcagtg
    15481 cattacgttc aatctgcttc ggcagcaggt gacgtttggc gatgttcagt ttttcatctt
    15541 cggtataacc ggagaggcga atcacttcca tacgatccag cagcggtgcc ggaatgttca
    15601 tggagttcga cgtcgcgaca aacatcacgt cgctgagatc gtaatccact tccaggtagt
    15661 ggtcgctgaa cgctacgttc tgctctggat ccagcacttc aagcagtgca gaggccggat
    15721 cgcctcgcat gtcagaagac attttgtcga tctcatcgag caggaacagc ggatttttca
    15781 cgcccacttt cgccattttc tggatcagtt tacccggcat agaaccgatg taagtacggc
    15841 ggtgaccacg gatttccgct tcatcacgca cgccgcccag cgccatacgg acatatttac
    15901 gcccggtggc tttggcaatg gactgaccaa gagaggtttt acctaccccc ggcggcccta
    15961 ccaggcagag gatcggtccc ttgattttgt tgacacggct ttgaaccgca aggtactcaa
    16021 ggatgcgatc tttcacgcgc tccagaccat aatggtcggt atcaaggatt tcctgcgcct
    16081 gacgcaggtc ttttttgacc ttgctgcgcg cattccacgg tacctgtacc atccagtcga
    16141 tataaccacg cactacggtc gcttctgccg acatcggaga catcattttc agcttctgca
    16201 actctgcttc cgctttctct tttgcctctt tcggcatctt cgccgcgtcg attttgcgct
    16261 tcagggcttc gttttcgtcc ggcacgtcgt ccatttcacc gagttctttc tgaatagctt
    16321 tcatttgctc gttcagatag tactcacgct gggatttctc catctgcttt ttaacgcggt
    16381 tgcgaatgcg tttctcaacc tgcagcagat cgatttccga ttccatcatt gccatcagat
    16441 attccagacg ttcgttaacg tcggacatct ccagaacgga ctgtttgtca gccagtttca
    16501 gcggcatatg tgcagcaatg gtatccgcca gacgcgccgg atcgtcgatg ctattcagcg
    16561 acgtcagcac ttctggtggg atttttttgt tcagcttgat gtagccttcg aactggctga
    16621 ttgcagtacg caccagcact tcctgttccc gctcatcaat ggtcggcgac tccagatact
    16681 ccgccttcgc agaaaagtgt tcgccattgt cagagagcgc agaaatacgc gcgcgctgta
    16741 acccctcgac cagcactttg acggtgccgt caggcagttt cagcatctgc aatatagagg
    16801 ccacggtccc gacggtgaaa agatcgttta cacccggctc atccgttgaa gcttctttct
    16861 gcgcgaccag cataattttt ttatcatggt ccatcgccgc ttccagacaa cggatagatt
    16921 tttcccgccc gacaaataag gggatgacca tgtgcggata aaccaccaca tcgcgcagcg
    16981 gcaatacggg gatttcaatg cgttcagaac gctcaggatt catagagctc tctcttagtt
    17041 taatttccgc caggtaatca gatgacacga ctgtgcttca cgccatctat taacatgtac
    17101 gtcagtatat ggggatgttt cccccacatt caacgccgag aatagaggaa aaattaaagg
    17161 ggagataaaa tccccccttt ttggttaact aattgtatgg gaatggttaa ttattcacca
    17221 gatgcctgtt gcgcttccgg cttaccataa atcagcaacg gtttgctttg accatcaatt
    17281 accgactcgt cgataaccac tttttcgacg tcttccatgg acggcagatc gtacatggta
    17341 tcgagcagtg cggcttctac gatggaacgc aggccacggg caccggtttt acgcgccatc
    17401 gctttcttag cgatagcatc cagcgcctcg tcacggaatt ccagatccac gccttccaga
    17461 ttaaacagcg cctgatactg cttggtcagg gcgtttttcg gctctttgag gatctgaatc
    17521 agagcttctt cgctcagttc attcaacgtt gctacaaccg gcagacgacc aatgaactca
    17581 gggataagac caaacttgat cagatcttcc ggttcaacct gcgccagcag ctcgccttcg
    17641 cttgctttgt cggacttcgc ttttaccgtc gcgccaaaac caatgccgga gccggtttct
    17701 acacggtggg aaatcacttt atccagaccg gcaaacgcac cgccacaaat aaacaggatc
    17761 ttagaggtat caacctgcaa gaattcctgc tgcggatgtt tacgcccacc ttgcggtgga
    17821 acagcagcta ccgtaccttc gatcagtttc aacagtgcct gctgtactcc ttcaccggaa
    17881 acgtctcggg taatggacgg gttgtctgac ttacgagaaa tcttgtcgat ttcatcgatg
    17941 tagacaatcc cgcgctgcgc tttctgtacg tcgtaatcgc acttttgcaa cagcttctga
    18001 atgatgtttt caacgtcttc gcccacataa ccggcttcgg tcagcgtggt tgcgtcggcc
    18061 atggtgaacg ggacgtccag caggcgcgcc agcgtttcag ccagcagcgt tttaccggaa
    18121 ccggtcggac cgatcagcag aatgttactt ttgcccaact cgacaccatt gctggtatcg
    18181 ccgttgcgca gacgtttgta atggttgtat accgcgaccg ccagcacttt tttcgcctgt
    18241 tcctggccga taacgtaatc gtccaggtgg ttgcgaattt catgcggcgt cggtagcgca
    18301 ctgcgttcac gatgcggtgc aacttcttta atctcttcgc gaatgatgtc gttacataaa
    18361 tcaacacatt cgtcgcagat atacacggat ggaccggcaa tcagcttgcg cacttcatgc
    18421 tggcttttgc cgcaaaaaga gcaatacagc aatttgcctg agccatcttt gcgtttatct
    18481 gtcatgggtc aaaacctctt ctttgttctt tgtgccgcac acgacgacgc aaatgccatt
    18541 ctcaggcgca agccgcttac agcgttgtgc cgccctagat aagtatagcg gcacagttgc
    18601 gcctctggca tcaattacga tgggtcagaa tcgaatcgac cagaccgtat tccaccgctt
    18661 caggggcgga aaggaagcga tcgcgctcgg tatcacgttc aatctgttct aatgattgac
    18721 ccgtatgaag cgccataagt tcattcatgc gccctttaac tttcagaatt tcacgggcat
    18781 gaatttcgat atcggtcgcc tggccctggt agccgcccaa cggttggtga atcatcacgc
    18841 gcgaattcgg caggcaaaaa cgtttacctt ttgcccctgc ggtcaacaag aaagcgccca
    18901 tcgaggccgc ctggcccata cagatggtgc tgacatcagg cttgataaac tgcatggtgt
    18961 catagataga catcccggca gtgatcaccc cgcctgggga gttaatgtac agatagatat
    19021 ctttttctgg gttttccgct tccaggaaca gcatctgcgc cacaatcagg ttagccatgt
    19081 ggtcttcaac ctggccagtc agaaaaatga cgcgttcctt aagtagacga gaatagatat
    19141 caaaagagcg ctcaccgcgt gaggtctgtt caatgaccat cggcaccagc gccatatggg
    19201 gtgcaaagtt atctcgttcg ccgctgtatg acatttccgt ctcctggata aaattgaaaa
    19261 aaacctgctg taccgattgt aaccttatgg acggattatc agctagtccc tgtgttatgg
    19321 gtacggcatc accatatttc aagcataaca atcttttgtt gttacgctaa caccgaaaga
    19381 ggcttttcgc acggctccat gcaaaattca tgacaaaaaa aagcccacca cctggcggtg
    19441 acgggccttt gtgcgaattt agcgcgttat gctgcgtaaa ttacgcctgc tggttcatca
    19501 gctcgttgaa agtggtttct ttttcagtca ctttcgcttt cgccagtaca gcttcaacag
    19561 cctgttcttc cagagcaaca ttgcgcatgt tgtccatcag ttctttgttt ttgctgtaga
    19621 actcgataac ttctttcgga tcttcgtacg cagaagccat ctcttcgatc aggcctttca
    19681 cgcgctcttc gtcagctttc agctcgttgg tgcggataac ttcgcccagc agcaggccaa
    19741 caactacgcg gcgtttagcc tgttcttcga acagttcgcg cggcagttcc agagcttgtt
    19801 tttcgttgcc accgaaacgc tgtgcagcct ggcgacgcag aacgtcgatt tcgctgtcga
    19861 tcagcgcagc cggtacgtcg atgtcgttag cttttaccag accttcgatc gcctgagact
    19921 taacgcggtt acggatggcg ctcttcagct cgcgctccat gtttttacgc acttcagcgc
    19981 gcagaccttc tacggaacca tcttcaacgc cgaaacgttt gatgaattct gcagtcagtt
    20041 ccggcagttc acgctcttca actttcttca ggttgatagc gaatttcgct gctttacctt
    20101 tcaggttttc tgcgtggtat tcttccggga aggtcacgtc gatggtgaac tcttcgccag
    20161 ctttgtggcc tttgataccg tcttcaaagc ccgggatcat acgaccctgg cccatcgcca
    20221 gtacgaaatc agacgcttta ccgccttcga actcttcgcc gtctacagaa ccggtgaagt
    20281 cgatggttac gcggtcttct gcttcaacag cgccgtcttt ttctttccag gtcgcctgct
    20341 gtttacgcag agtatccagc atgccgtcaa cgtcagcgtc ggtcacttca acgatcggtt
    20401 tttcaacttc gatcgcttcc agaccctgca gttcaacttc cggataaact tcaaactcta
    20461 cagagtaagt gaagtcttca cccagcttgt attcgcccgg aacataagtc ggtgcgccag
    20521 ccggattgat tttttcttta atgatggcgt caatgaagtt acggctcatc aggtcaccca
    20581 gaacgtcctg gcgtacagac gcgccataac gctgagcaac gatattcatt ggcactttgc
    20641 ctttgcggaa gccgtcaata cgtacttttt tcgcaacgtt gaccagctcg cttttaacag
    20701 cggtctcgat gctgtcagca gcgatagtaa tcgttacacg gcggccaagg ccttgagtgg
    20761 tttcaactga aacttgcatc ttgttacctc aaaaaatcac agtgctcggt caactcttac
    20821 cccgcaagca caggttgttg gcttcgcgga accgggatgt tcttataatc aatcacattc
    20881 cctgttacca gaatcatccc gaaagcattc aaataggacg cggcattata gcggcatcac
    20941 ttttatgagt cgagaacggt tattgcgcgc tgtcgcgtat tttttcacaa ttcccgggca
    21001 attttcgtct gaatgctggg caaaaagcgc aaaatccaga caaaacaaaa cggcccgcag
    21061 gccgtttttc ataaaatctt tagcaacata ctggaaaagc gccgacagtt gcaaatgcgt
    21121 ttttacgcga tgcttcctgc tccacgacag ggaggagagg caaagacggt gtcctgcaac
    21181 ccttcccact ccttaatagt gtaagtatgc agagccagcg catgaacggt agtagagagt
    21241 tcctccgcta aagtactgta aatcattcga tgacgattca gaaaacgttc acccgtaaaa
    21301 cgatcgctga ccagcacaac tttaaaatgg ctttcagagc cggctgggac attgtgacga
    21361 tagctttcat ccactacttc gaggaatacg ggttggaacg ccgcccttaa tttttcttct
    21421 atccgctcac gtatcatcat gaaatatctc ctccgacaac gctgagatgt catccatccc
    21481 tttaaatact agccgctttt accgtttcca ttgcagctta acaacaaata tttaggtttc
    21541 gtcctggttt ttccgtcaag cgtatgaagt ttactgattt aaagattgaa acacaggcct
    21601 cgcatcagcg tttcttttac caggggtgat tatttcgtca acatgatgaa tttacatgcc
    21661 ttacccactt cccctcgccg ttggcaatgt tatgatggcg ggaattttta ccaaacacgc
    21721 ttaagagtct ctgagagccc gaacatgttc aaaaaaatcc tcttcccgtt agttgctctg
    21781 tttatgcttg caggatgcgc aaaaccgcca acaactattg aagtttcccc gacgattaca
    21841 ctgccacagc aggatccaag cctgatgggc gtcaccgtaa gcattaatgg tgccgatcag
    21901 cgtaccgatc aggcgctggc aaaagtcacc cgcgataatc aaatcgttac cctgaccgcc
    21961 tcccgcgctc tgcgtttcct gctgcaagaa gtgctggaaa aacagatgac cgcgcgtggt
    22021 tacatggttg gtccgaatgg cccggttaat ctgcaaatca tcgttagcca actgtatgct
    22081 gacgtgtccc agggcaacgt gcgctacaac atcgctacca aagcggatat cgccatcatc
    22141 gctaccgcgc agaatggcaa caaaatgacc aaaaactatc gtgccagcta caacgttgaa
    22201 ggtgcgttcc aggcctccaa caaaaatatc gccgatgcgg ttaacagtgt gctgaccgat
    22261 accatcgctg atatgtctca ggacaccagc attcacgaat tcatcaagca gaacgcgcgt
    22321 taatttctgc cctctggccc ggtgcaagcc gggcctgtag acgcccatgt ccagtcaata
    22381 tttacgtatt tttcaacagc cgcgttcagc catattgctg atcctgggtt ttgcttccgg
    22441 gctaccgctc gccctgacat ccggcacctt acaggcctgg atgacggtcg aaaatatcga
    22501 tctcaaaacc attggtttct tctctctggt aggccaggct tacgttttta aattcctctg
    22561 gtcaccgctg atggaccgct acacgccccc attttttggg cggcggcgcg gttggctgct
    22621 cgccacgcaa atcctgttat tagtcgccat tgcggcgatg ggttttctcg aaccaggcac
    22681 ccaactccgc tggatggcgg cgctggcggt ggtgatcgct ttttgctctg cctcccagga
    22741 tattgtcttc gatgcgtgga aaaccgatgt gctcccggca gaagaacgtg gtgcgggcgc
    22801 ggcaatcagc gtgctgggtt accgtttagg gatgctggtt tccggcggcc tagccctgtg
    22861 gctggcagat aaatggttgg gctggcaggg catgtactgg ttgatggcgg cgctgttgat
    22921 cccctgtatt atcgcgacgt tgcttgcacc agaaccaacc gacaccattc ctgtgcccaa
    22981 aacgctggaa caagcggttg ttgcacctct gcgagatttc tttggtcgca ataatgcctg
    23041 gcttattttg cttcttatcg tgctgtataa gctgggcgac gcattcgcca tgagcctgac
    23101 aaccacgttt ttgattcgcg gcgtcgggtt tgatgcgggt gaagtaggcg tggtaaacaa
    23161 aacgcttggc ttactggcga ccattgttgg cgcattgtac ggtgggattt tgatgcagcg
    23221 cctgtcgctg ttccgggcgc tgctgatttt cggcatttta caaggtgcgt ctaacgctgg
    23281 ttactggctg ctgtcgatta ctgataagca tctctacagc atgggcgcag ccgtcttttt
    23341 cgaaaacctc tgtggcggga tgggcacatc agcctttgtc gcgctgttaa tgacgctgtg
    23401 taataagtca ttttccgcta ctcaatttgc cctgctctca gcgctttccg ctgtagggcg
    23461 agtttatgtc ggccccgtgg cgggttggtt tgttgaagca cacggctggt cgacattcta
    23521 tctattctcc gtcgccgctg ccgtaccggg gcttattttg ctgctggttt gccgccagac
    23581 gcttgaatat acacgagtaa atgacaactt tatctcccgt accgaatatc cggcaggtta
    23641 tgcctttgcc atgtggacac tggcggcggg cgtcagcctg ttggccgtgt ggttacttct
    23701 gttgacgatg gacgcgctgg atttgacgca cttctctttc ctgcctgctc tgctggaagt
    23761 cggggtttta gtcgcccttt ctggcgtcgt gcttggtggt ttgctggatt atctggcgct
    23821 acgaaaaacg catctgacgt aatctgtaaa tattatttag ctgtagtaat cactcgccgt
    23881 aattattacg gctaaataaa catcagcatc gcttattaat atttgttctt ttgtgcggct
    23941 tagcgtttgg aaattattga cgccatttat aaattctatt tttcttacac gattcagcta
    24001 atgagtcttt attttctcac cacccagttg tcactttaat gataattatt tgttaaataa
    24061 ttgttttatt tcgcattggt tataccaatt gcccaccaag actccttctg cactcccctt
    24121 ttgttata
//
LOCUS       DAJTQQ010000058        23844 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000058 DAJTQQ010000000
VERSION     DAJTQQ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23844
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.058"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(66..938)
                     /gene="rluF"
                     /locus_tag="OO309_002509"
     CDS             complement(66..938)
                     /gene="rluF"
                     /locus_tag="OO309_002509"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR5847619.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1286..2233
                     /locus_tag="OO309_002510"
     CDS             1286..2233
                     /locus_tag="OO309_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR5847620.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2303..3109
                     /locus_tag="OO309_002511"
     CDS             2303..3109
                     /locus_tag="OO309_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5847621.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3119..3526
                     /locus_tag="OO309_002512"
     CDS             3119..3526
                     /locus_tag="OO309_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR5847622.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3526..4020
                     /locus_tag="OO309_002513"
     CDS             3526..4020
                     /locus_tag="OO309_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR5847623.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4086..4883
                     /locus_tag="OO309_002514"
     CDS             4086..4883
                     /locus_tag="OO309_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR5847624.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            4894..5682
                     /locus_tag="OO309_002515"
     CDS             4894..5682
                     /locus_tag="OO309_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR5847625.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5734..6963
                     /gene="sorE"
                     /locus_tag="OO309_002516"
     CDS             5734..6963
                     /gene="sorE"
                     /locus_tag="OO309_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR5847626.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7345..8034
                     /gene="pepE"
                     /locus_tag="OO309_002517"
     CDS             7345..8034
                     /gene="pepE"
                     /locus_tag="OO309_002517"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR5847627.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8125..9756)
                     /locus_tag="OO309_002518"
     CDS             complement(8125..9756)
                     /locus_tag="OO309_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR5847628.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(9976..13659)
                     /gene="metH"
                     /locus_tag="OO309_002519"
     CDS             complement(9976..13659)
                     /gene="metH"
                     /locus_tag="OO309_002519"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR5847629.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            13859..14683
                     /gene="iclR"
                     /locus_tag="OO309_002520"
     CDS             13859..14683
                     /gene="iclR"
                     /locus_tag="OO309_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR5847630.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            15000..17184
                     /gene="espL4"
                     /locus_tag="OO309_002521"
                     /pseudo
     CDS             15000..17184
                     /gene="espL4"
                     /locus_tag="OO309_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(17153..18890)
                     /gene="aceK"
                     /locus_tag="OO309_002522"
                     /pseudo
     CDS             complement(17153..18890)
                     /gene="aceK"
                     /locus_tag="OO309_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(19074..20378)
                     /gene="aceA"
                     /locus_tag="OO309_002523"
     CDS             complement(19074..20378)
                     /gene="aceA"
                     /locus_tag="OO309_002523"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCR5847631.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20408..22009)
                     /gene="aceB"
                     /locus_tag="OO309_002524"
                     /pseudo
     CDS             complement(20408..22009)
                     /gene="aceB"
                     /locus_tag="OO309_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(22278..23207)
                     /gene="metA"
                     /locus_tag="OO309_002525"
     CDS             complement(22278..23207)
                     /gene="metA"
                     /locus_tag="OO309_002525"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR5847632.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            23364..23807
                     /locus_tag="OO309_002526"
     CDS             23364..23807
                     /locus_tag="OO309_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR5847633.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5865 a         6081 c         6055 g         5843 t
ORIGIN      
        1 tccccgaagg ggcgagggga ctgtccgagc tctttttgga cgttttcatc agtctgaggc
       61 gttaatcagc gtcccttctt tttacgcccc ggcgaggtaa aacgcttacc gttggacgcc
      121 gggcgaccgc ctttttccgc cgttttttcc atcttaacga ctggacgttt gatgcccgct
      181 gttttcggtt tggccttcgc tttaggtttt gcctcggaag aggaattttc gatgagctta
      241 aagaggtcga ttaactcatc gtcggttaaa tcgcgccact cccccagcgg aatgccgctt
      301 aagctgacgt tcataatgcg cgtacgttcc agctttttca cttcatagcc gaaatgctcg
      361 cacatgcgcc ggatctgacg gttcagcccc tgcactaggg taatgcggaa gacaaacggc
      421 gcttcttttt tgactttgca ctttttcgtc accgtcccga ggatcggcac ccccgccccc
      481 ataccacgaa taaattcatc ggtaatcggt ttatcgaccg tcaccagata ctctttctca
      541 tgatcgttac ccgcacggag gatcttattc accagatcgc cgtgattggt gaggaaaatc
      601 agcccctggg agtctttatc caggcggcca atcgggaaca cgcgtttgct gtggttaacg
      661 aaatcgacaa tgttatcgcg ctcgccatct tcggtggtgc tgacaatacc gacgggcttg
      721 ttcagggcga taagtaccaa atcttcggct tcccgaggct caatcaactg accgtttact
      781 ttcacaatgt cgccgggttt cacctgatcg ccaatggtgg ctcgcttgcc attaaggaac
      841 acattgcctt gctcgatata gcgatccgct tcgcggcgtg agcaaattcc gctttcgctg
      901 atgtatttat ttaaacggac tgatgagtcg ggcagcataa attctcctgt aaaacgcgga
      961 atataccgta cctcttagcg gggaaaaaca gggttatgcg gcaacgtcga aattaaatgt
     1021 gatctgccac gccttttatc acaaaagtgt ctccggaagt acgatgcaaa tttgcctggc
     1081 aaaataagga taatcaacat attacgaatc gctgcacacc tgtgctgatg actactacgc
     1141 agagaaacaa atgtgcaaga gaaagcgcta tttctcccca tgcaaagagt gaaaaatcgc
     1201 tttgaaaggc cctcatgatg gggttatatc agatcaagcg gtgagaaagc gtatttgcac
     1261 aaatgtgcag aaccaggagg cggcaatgga aaacagtgac gatatccgtt tgattgtgaa
     1321 gattgcccaa ctctattacg aacaggatat gacgcaggcg caaatcgcgc gcgaactggg
     1381 tatttaccgc accaccatca gccgcttgct taaacgaggc cgcgatcagg gaatggtcac
     1441 catcgccatc aactatgact acaacgaaaa tctctggctg gagcagcaac tgaagcaaaa
     1501 gtttggcctg aaagacgttg tggtggtgtc gggaaatgat gaggatgaag agactcaact
     1561 ggcgatgatg gggttacacg gcgcgcaact gctggatcgc ttgctggagc ctggcgatat
     1621 tgtcggtttt tcctggggtc gcgcggtgag cgcactggtt gaaaacttgc cgcaggcggg
     1681 gcaatcgcgg cagttaatct gcgtaccaat tattggcgga ccgtccggta aactcgaaag
     1741 ccgctatcat gtaaacacat taacctacag cgcggcagcg aagctgaaag gggaatcgca
     1801 tctcgcggat tttccggctc tgctggataa cccattaatt cgtaatggga tcatgcagtc
     1861 tcagcacttt aaaaccatct ctgcctactg ggataatctg gatatcgccc tggtgggaat
     1921 tggctcaccg gccattcgcg acggcgctaa ctggcatgcg ttttatggtg gtgaagagag
     1981 tgacgacctg aatgcccgcc aggttgctgg cgatatttgc tcgcgctttt ttgatattca
     2041 cggcgcaatg gttgaaacga atatgagcga aaaaacgctc tctatcgaaa tgaataaatt
     2101 aaagcaggcg cgatattcca ttggcattgc catgagtgaa gaaaaataca gcggaattat
     2161 tggtgcactg cgtggaaaat atattaattg tctggtaacg aatagcagca cagctgaact
     2221 attactgaaa taaaaatctt cccgatgttt accgtaataa aaattacggc gggtccctat
     2281 tatctaaaat caggagtctg ttatgcaaac gtggttaaat ttgcaggata aaatcattat
     2341 tgtcaccggc ggcgcatccg gtattggtct ggcgattgta gaggaattat tagcacaagg
     2401 cgcgaatgta cagatggtcg atattcacgg tggcgatggt caatatgaag gtcataaagg
     2461 ctatcagttt tggccgaccg atatttctag cacaaaagag gtaaatcata cggtagcgga
     2521 aattatccag cgttttggtc gcatcgacgg tctggtcaat aacgccgggg tcaatttccc
     2581 gcgtctgctg gtcgatgaga aagcgcctgc cgggcagtat gaactcaacg aagctgcatt
     2641 cgaaaaaatg gtcaatatca accagaaagg tgtttttctg atgtcgcagg cggtggcgcg
     2701 acagatggtc aaacaacatg atggcgtgat tgtgaatgtt tcctcagaaa gtgggctgga
     2761 aggctcagaa ggacaaagct gttacgccgc taccaaagcc gcgctcaata gcttcacgcg
     2821 ctcctggtcg aaagagctgg gtaaacacgg tatccgtgtg gtcggtatcg cgcccgggat
     2881 tctggaaaaa accgggctac gcacgccgga atatgaagag gcgctggcgt ggacgcgcaa
     2941 tatcaccgtc gagcagctgc gtgaaggcta taccaaaaac gccattccta ttgggcgcgc
     3001 cggaagatta gcagaagtag ctgattttgt ttgttatctg ttgtctgaac gcgccagcta
     3061 catcaccgga gtaaccacta acattgcggg cggcaaaacg cgcggctaag gaggctttat
     3121 ggtcaacgcc attttttgtg cccacggcaa attagcttgc gccatgctgg aatcggttca
     3181 gatggtttac ggcaacgcca atgttgaagc cgttgcattt gtgcccggtg aaaacgccgg
     3241 agacattgcg gtaaagctgg aaaagttagt aagcattcac aatcaagatg agtggctgat
     3301 tgccgtggat ttacagtgcg gcagcccgtg gaatgcggca gcaatgcttg cgatgcgcaa
     3361 tcctcgtttg cgggtaatca gcggcctttc tctgccgctg gcgctcgaac tggttgataa
     3421 ccaggacagc atgaatgttg atgaactgtg tgaacacctg acgcaaatcg caaagcaaac
     3481 ctgcgttgtc tggaaacaac tggcaacgac agaggaggat ttctgatgaa cattacgctc
     3541 gcccgcattg atgaccgtct gatccacggc caggtcacta ccgtctggtc gaaagtggct
     3601 aacgcccagc gcattattat ctgcaatgac gaagtttata acgatgaagt gcgccggaca
     3661 ttattgcgtc aggctgcgcc accgggaatg aaggttaacg tggtcaatat tgaaaaagcc
     3721 gtcgcggttt atcacaaccc gcaatatcag gacgaaaccg ttttttatct tttcactcgc
     3781 ccacaggatg ccttagcgat ggttcgccag ggggtaaaaa taggcaccct gaatattggc
     3841 ggcatggcct ggcggcccgg taaaaaacag ttaaccaaag cggtttcatt agatgatgat
     3901 gatattaacg catttcatga gttaaataat cttggtgtca tactggattt acgcgtcgta
     3961 gcgtcagatc cctcaatcaa tattatcgac aaaataaacg aacagttaat cgcaaattaa
     4021 ataatagcgc ctgtgtacat atgctttaac aggcagggat gacgacactt aaaaggtgac
     4081 aaatcatgga aataagtacc ctacaaatca ttgctatatt tcttttttcc tgtattgccg
     4141 gaatgggcag cgtgctggat gaatttcaga ctcatcgccc gttaattgcc tgtacggtga
     4201 ttggtttaat tctcggtgat ttaaaaaccg gaataatact cggcggtacg ctggaattga
     4261 tagctctctg ctggatgaac gtcggcgcgg cgcaatctcc ggattctgca ctcgccagca
     4321 taatctccgc cattctggtt atcgttggtc agcaaagcat cgccaccgga atcgccatcg
     4381 cgttgcctgt ggctgcggca ggccaggtgc tgacggtgtt tgcccgtacc atcaccgtag
     4441 tgttccagca cgcggcggat aaagcagcag aagaggcgcg gtttcgcact ctcgatattc
     4501 tgcatgtctc cgcgcttggc gtgcaggcgc tgcgcgtcgc cattccggca ctgattgtct
     4561 cactgttcgt cagcgccgat atggtcagca atatgttaaa cgccattccc gaatttgtga
     4621 cccgaggact acagattgct ggcggtttta tcgtggtggt cggttacgcc atggtgctac
     4681 gtatgatggg cgtgaaatat ttgatgcctt tctttttcct cggtttcctc gcaggtggct
     4741 acctcgatct cagcctgctg gcgttcggtg gcgtcggcgt gatcatggcc ctggtctaca
     4801 tccagctaaa tccacagtgg tgtaaagctg aacctcaact ccaggccacc gcctccaccg
     4861 cccttgacca acttgacgat taacggagcc atcatggaac agagaaaaat tacacgcagc
     4921 gatctggtga gcatgtttct gcgctccaac ctgcaacagg cgtcctttaa ctttgaacgt
     4981 attcacgggc tgggcttttg ctacgacatg atccccgcca tcaagcgact ttacccatta
     5041 aaagaggatc aggttgcggc gctcaggcta cacctggtgt tcttcaatac cacgccagcc
     5101 gtatgtggcc cggtcatcgg cgcggaaatt gatgacggta ccatcaacgg catcaaagtc
     5161 ggtctgatgg gaccattggc aggagttggc gatccactgg tctggggaac gctgcgcccg
     5221 attaccgccg cgctcggcgc atctctggca ctttcgggca acattctcgg cccgctgctg
     5281 ttcttcttta ttttcaacgc ggtgcgtctg gcgatgaagt ggtatggcct acagctcggc
     5341 tttcgcaaag gggtgaatat cgtcagcgat atgggcggga atgtgctgca aaaactcacc
     5401 gaaggcgcgt cgattctcgg actgtttgtg atgggcgtgc tggtgaccaa atggacgtca
     5461 atcaacgtac cgttggtggt ttcacaaacg catgccgccg atggctccac cgtcaccatg
     5521 accgtgcaga acattctcga ccaactttgc cctggtttgc tggcgctcgg tctgacgcta
     5581 ctaatggttc gtctgctcaa caaaaaaatt aacccggtat ggctgatttt cgccctgttt
     5641 ggcttaggga ttatcggcaa tgctctgggc ttcctgtcct gattcttcgc cccggcacga
     5701 ctgccgaggc catcgctcaa catgaggtgg tttatgaaaa caacagctct gcgtctttat
     5761 ggtaaacgtg atttacgcct ggaaaccttt gaccttcctg aaatgcagga ggatgaaatc
     5821 ctcgcaacgg tggtcactga cagcctgtgc ctctcttcct ggaaagaggc caatctgggt
     5881 gaaaaccata aaaaagtacc cgacgatgtg acgaccaacc caatcatcat cggccacgaa
     5941 ttttgcggcg atattctggc cgtgggtaaa aagtggcagc acaaattcca gccgggtcag
     6001 cgttatgtga ttcaggccaa cctgcaactc cccgaccgcc cggattgccc cggctactcc
     6061 ttcccgtggg tagacggcga agcgacgcat gtggttattc ccaacgaggt catggaacaa
     6121 tattgcctgc tggcatacga cggcgaaacc tattttgaag gctcactggt tgaaccgctt
     6181 tcctgcgtga ttggcgcgtt caacgccaac tatcatcttc aggaaggtag ttataaccac
     6241 acgatgggga ttcgcccgca agggcacacg ctgatcctcg gcggcaccgg accaatggga
     6301 ctgttggcga ttgattatgc gctgcatgga ccagttaacc cgtcgctgct cgtcattacc
     6361 gataccgaca acgacaaatt gagttatgcg cgcaagcact atccatcaga accgcaaaca
     6421 ctgattcatt atctcaatgc caccgatgca gcatttgata cgttaatggc gctgagtggc
     6481 ggtcacggct tcgatgatat tttcgtcttt gtgcctaatg aaggactggt gactctcgcc
     6541 tcttccttgc tggcgacaga tggttgcctg aatttcttcg ccggaccgca ggataaacat
     6601 ttcagcgcgc caattaattt ctacgatgtg cattatgcat ttacccacta cgtgggcacg
     6661 tcaggcggca ataccgacga catgcgcgcg gcggtcaaac tgatcgaaga gaaaaaagtg
     6721 caggccgcaa aagtggtaac acatattctt gggctgaatg ccgcgggcga aaccacgctt
     6781 gaattgcctg ccgtcggcgg cggcaaaaag ctggtgtata ccgggaaata cctgccgctg
     6841 acgtcactca cgcagattca ggatcaagca ctggcggcga ttctggcgcg tcatcagggg
     6901 atctggtccg gtgaggtgga gcaatacctg ctcgcccatg cagaggcaat ttcccatgat
     6961 taatcgcgct acttaactct gcatgtcgct ggctggtcgc cccagcaact tcgcgaccgg
     7021 atgtgcggct gattgctgaa gcagcacaat ttgcacgaga aggataaaaa atatcagcgc
     7081 cttaaggggc gctcaaattg gtggcaaagt acacattgtt tatgccggat gcggaatgaa
     7141 gccttattcg gtctacataa ttttgaaaat ccaatatatt gcagttatct ggtaggccag
     7201 gtaagcgtag cgcatcaggc aatgctgtgt ttgtcatcag tctcagcgcc cggtgcaata
     7261 cgtgacatcg tgagccatgt cagtaagtgg ttaaattcgc agtggtagca tgccttccgt
     7321 gatttcttac taaccggaga agtaatggaa ctgcttttat tgagtaactc gacgctgccg
     7381 ggtaaagcct ggctggaaca tgcactgccg ctaattgctg aacagttgca gggtcgccgc
     7441 tcagcggtgt ttatcccttt cgctggcgta acgcagacct gggatgatta cacagagaaa
     7501 acggctgcgg ttctcgctcc gctgggtgtt tctgtcaccg gtattcatag cgttgtcgat
     7561 cccgttgccg cgattgaaaa tgcggaaatt gtgattgtcg gcggcggtaa tactttccag
     7621 ttgctgaaac agtgccgcga gcgcgggctg ctggctccaa ttactgacgt ggttaaacgt
     7681 ggcgcgctgt atattggctg gagcgcaggc gctaaccttg cttgcccaac cattcgtacc
     7741 accaacgata tgccgattgt cgatccgcaa ggtttcgatg cgctaaatct gttcccgttg
     7801 caaatcaacc cgcacttcac caacgcgctg ccggaaggcc ataaaggtga aacccgtgag
     7861 cagcgtattc gcgaactgct ggtcgtcgcg ccagaactga cgattattgg tctgccggaa
     7921 ggtaactgga tcacagtgag taaaggtcac gctacgctgg gtggcccgaa caccacttat
     7981 gtgtttaagg ctggtgaaga agcggttccg ctggaagctg gtcaccgttt ttaagtgaat
     8041 tgccggatgc atgatccggc atcttgttca tagtgcctga tgcgacgctt gacgcgtctt
     8101 atcaggccta caggattcac aatattaata ctcgtcccgt ccttcatctt catccggctg
     8161 ttccagcaca ctgtaagcca ccgaacaaaa cagcgagttc aggcgctgca tatctcccag
     8221 taagcctaaa tgtagcgaac tggtttcaat gctctgcacg ttttgctgat gcaggcgatc
     8281 gacgtgggcg tgggaatagc ggcgattaag aatgcgaaaa cgatgtttgc tgcgacgcaa
     8341 acgacgagcg ctggtgacat cgccagagaa gaacaccgac attgccagct ttaaattact
     8401 gagcaattgc tcatagagcg catccagttc cttcaaccca tcaagcgaaa atgcccgccg
     8461 tgctgccagc gatttatcgg caatttcgct gcccatgcgc tcgacgatat cggaggcctg
     8521 ttcaaggttg agcgacattt cgatgatctc cgcccagcgg cgcgactctt cctccgccaa
     8581 ctcttctttt ggcatccgcg ccagatacag cttaatggcg gtgtagagaa cgttgatatc
     8641 atctgccagc ttacgcagct ctttctcctg ccgtggctcg ccgtgcatca ctttattcag
     8701 cccttccatc atctgttcca tggcgtcgcc aatgcgcagg gtttcgcgcg cggcgttcgc
     8761 cagagcaagc gtgggcgtgt ccagcgcgct gacatccaga tgtttaggcc gtagctgggt
     8821 atccagttcc ggttcatcgc gaataatcgt tttgcaaaac cgtgccatcg ggtcaacaaa
     8881 tggcagcatg accaggcaac gtacaaggtt gtagaagacg tggaaataga tcaccagttc
     8941 cgcttttggc aatgacagct tccccattgt ctctgccagc aaatggacaa acggcaggat
     9001 aatcaggcta cccaccagct taaacagcag actacccagc gcgacacggc gggctgcggc
     9061 attggcggca ctgttgttga gcatcgccag caggccggaa ccgaggttag ctccaatcac
     9121 cagacagagc gccacgggga aggagataat gcctgcagcg gtcagagtcg cggtcagcag
     9181 tacagcagca aggctggagt agctgataat ggcgaacatc gcgccaatca gcgcatccag
     9241 cagaatatcg ccggtcagcg aggcaaagat cacctgaacg ccgtttgcct gggtgatcgg
     9301 cgttacggcc tgcacaatca actccagcgc cagcaaaatc agcccaagac caataccgac
     9361 gcggcccagt tgcccggcgc gtgactgttt gcgtccgagg aaaaaaatca cgccgataaa
     9421 aataagtaac ggtgacagcc aggataagtc gaaggtgaga atacgcgcca ttagcgccgt
     9481 cccgacatct gcccccagca caatgaccag agccggtgcg agggctacca gatcctgagc
     9541 gacgaacgag gtcaccagca tggtggtggc attactgctc tgtaccagtg cggtaacgcc
     9601 gatccccgcg caaaaggcga gcggcttttt ttcgacgctc cggctaagga cagtacgcaa
     9661 acgcgcgccg aagacgcgca ttacgccggt tcgaacaata tgagtccccc agaccagcag
     9721 ggcgacggca gaaagcaggt gaagcagcgt taacacgagt tcagattctc cttatcgtta
     9781 tatgttcctg aagcaattga atgaccaaag gaccggaaac acgctgactg gcatctttac
     9841 tgtgcctttt gacattcgtt tttccggcta agattattcc agcctgtttc cagtataagt
     9901 gtttcatatt aataaagaga cagggcactc attgagcgcc ctgtttgttg taaggaaaag
     9961 tgacagattt gtgaatcagt ccgcgtcata ccccagattc ggtgccagcc agcgctcaac
    10021 atcggagacg ctcatacctt tacggcgggc atggtcttca acctgatcgc gctgaatttg
    10081 cgccaccgcg tagtacttgc tgtccggatg actgaaatac caacccgaaa ccgatgcacc
    10141 aggccacatc gcgaaagatt ctgtgagttt catgccagta tgtttttcca cttccagcag
    10201 ctcccagatg gtggcttttt ccgtatgttc cgggcaggcc ggatagcccg gtgccggacg
    10261 gatgccctgg tagttttcgc ggatcagttc ttcgttgctg aggttctcgt tcggcgcata
    10321 gccccagtag actttacgca cacgctcatg gagatactcc gcaaaggctt cggctaaacg
    10381 gtcggcaagc gctttcacca tgattttgtt gtaatcatcg tgctgcgctt caaaggcatc
    10441 agccagtgcg tcctcttcca gcccgccagt cacggcaaat gcgccgatgt aatctgcttt
    10501 accagaaagc ttcggcgcaa cgaagtcagc gagacagtag ttagcgaatc ccgttttttc
    10561 ggtctgttga cgcagatggt ggctgacgtt gatcacatgg gtgcgcgttt cgtcacggta
    10621 gatttcaatg tcatcgccca cacggtttgc cgggaacagg cccaccacgc cacgcggatt
    10681 cagcgttttc tcggcgctta atttatccag catgtcgttg gcgtctttaa acagccgctg
    10741 cgcctcaacg cccaccactt catcttccag aatgcgagga tacttcccgg ccagcgacca
    10801 ggtcataaag aacggtgtcc agtcgatgta attacgcagc gtttcgatgc tggcttccac
    10861 ttcctgcacg ccgagacggt gcgccaccgg cggcgtgtaa gcctgccagt caaatgcgaa
    10921 atcgttatcg cgcgccgctt ccagcgtgac cggtggtgtg cgtggtttct tgcgcccgtg
    10981 ctgaatacgc acggtttcgt actccttgca ggtacgagcg acaaaatcat cacgctgagt
    11041 atcggaaagc agcgccgcta ccacaccaac ggtgcgcgag gcattctgca catacaccgt
    11101 agggccgctg tagttctgct cgattttcac cgccgtgtgc gcttttgagg tggtcgcgcc
    11161 gccaatcagt aacggaatgg cgaagccctg acgctccatc tctttcgcca cgttaaccat
    11221 ctcgtccagc gacggcgtga taagccccga aaggccaatc aaatcagcat tcacttcttt
    11281 ggcgatacgg agaatttttt ccgcaggcac cataacgccg agatcgacaa tttcgtagtt
    11341 gttacattgc agcaccacac caacgatatt tttaccgatg tcgtggacat cgcctttcac
    11401 ggtggcgatc accatcttgc cgttggtttt accctgctct ttgctggctt caataaacgg
    11461 ttcgagatac gccactgcct gtttcatgac gcgcgccgat ttgaccacct gtggcaggaa
    11521 cattttgcct tcgccaaaca gatcgccgac cacgttcatg ccgtccatca acgggccttc
    11581 aatcacttca atcgggcgcg tagcctgctg gcgggcttct tcagtatcct gctcgataaa
    11641 ctcggttatg cctttgacca gcgagtattc cagacgttta ttcacgtccc acgagcgcca
    11701 ctccgcttgc tgggcgttag cagtgtcgtc cgctttactg ccgcgatatt tctcggcaag
    11761 ctccagtaaa cgctcagtgc catcgtcacg acgattaaga atcacatctt ccaccgcgtc
    11821 gcgcagttca gcgggtaggt cgtcgtaaat cgccagttgc ccggcgttga cgatccccat
    11881 atccatgcca ttgcgaatag cgtagtagag gaacactgcg tgaatggctt cgcgcaccgg
    11941 atcgttgcca cggaatgaga aagaaacgtt agatacgccg ccggaaatca gcgcgtgcgg
    12001 cagttcgcgt ttgatgtctt cacacgcgcc gataaagtcc tgcgcgtagt tgttgtgctc
    12061 ttcaataccg gtggcgaccg cgaagatgtt cgggtcgaag atgatatctt ctggcgggaa
    12121 gccaacctct tcggtgagga ttttgtacgc ccgacggcaa atctcgattt tccgtgcgcg
    12181 agtatcggcc tgtccctgtt cgtcaaaggc catcaccacc actgccgcac cgtagcgacg
    12241 caacaatttc gcgtgatgga taaaggcgtc gacgccctct ttcatcgaga tagagttaac
    12301 aatgcctttg ccctggatac acttcagacc tttttcaatg acatcccatt ttgaggagtc
    12361 gatcataatc ggcacgcgag cgatatccgg ttcaccggca atcagattga gaaaacgcac
    12421 catcgccgct tcggcatcga gcatcccttc atccatgttg atatcgataa tctgcgcgcc
    12481 gttttccacc tgctgacgcg ccacatccag cgcctcgctg tatttctctt ctttgatcag
    12541 gcgcttgaac ttagcagagc cggtgacgtt ggtgcgttca cccacgttca caaacaggct
    12601 atcgtcgcca atgttcagcg gctccaggcc agacaaacgg caggctacgg gaatttccgg
    12661 cagtttgcgc ggcgctaatc cttctactgc acgactcatc gctgcaatat gttgcggcgt
    12721 ggtcccgcag caaccaccga cgatattgag aaagcccgct tgcgcccatt cacgtatctg
    12781 ttttgccatc gtgtcggcgt cgagatcgta ttcaccaaag gcgttaggca gcccggcgtt
    12841 cgggtgggcg gtgacgtagc attccgcaat ccgtgatagt tcctgcacgt actggcgcag
    12901 ttcatcgggc cccagcgcac agttcaggcc aaaggtcagc gcttcggcgt ggcgcaatga
    12961 gttgtaaaat gcttcggtgg tctgcccgga gagcgtgcgc ccggaggcgt cggtgatggt
    13021 gccggagatc ataatcggca gctcaacgcc cagcgcttca aactccgttt tcaccgcaaa
    13081 gaccgccgct ttggcgttaa gggtgtcgaa aacggtttca atcaggatca gatccgcacc
    13141 accttccacc agcgctttgg tggattcgcg ataggccgcc gccagctggt caaaagtgat
    13201 gttgcggaat gccggatcgt tgacgtccgg agaaatagac gccgtgcggt tggttgggcc
    13261 aagaacaccg gcaacgtagc gcggtttctc cggcgtgcgc gcggtccact cgtcagcaca
    13321 ggcgcgcgcc agtttcgccg ccgcaaagtt gatttccgcc gacagggatt ccatctggta
    13381 atccgccatc gcaatggtcg tggagttgaa ggtgttggtt tcgatgatat ccgcgcccgc
    13441 ttcaaagtag gcgttgtgga tagcggcgat cacttccggt ttactgagta ccagcaggtc
    13501 gttgttgcct ttgaggtcgc atggccagtc ggcaaagcgt tcaccacgaa aatcggcttc
    13561 gttcagtcga tagctctgga tcatggtgcc cataccgccg tccaacacca gaatacgttc
    13621 atttaactgc gcacgcagtt gttccacttt gctgctcaca cttgctcccg acacgcttaa
    13681 cccagattta ataaaaattc aacaaaccat actggaataa acgcatctgt ggtaaaagcg
    13741 accaccacgc aacatgagat ttgttcaaca ttaactcatc ggatcagttc agcaactatt
    13801 gcattagcta acaataaaaa tgaaaataat ttccacgata cagaaaaagg aggctgtcat
    13861 ggtcgcaccc attcccgcga aacgcggcag aaaacccgcc gttgccaccg caccagcgac
    13921 tggacaggtt cagtctttaa cgcgtggcct gaaattactg gagtggattg ccgaatccaa
    13981 tggcagcgtg gcactcacag agctggcgca acaagccggg ttacccaatt ccacgaccca
    14041 ccgcctgcta accacgatgc aacagcaggg tttcgtgcgt caggtcggcg aactgggaca
    14101 ctgggcaatc ggcgcacatg cctttatggt cggcagcagc tttctccaga gccgtaattt
    14161 gttagcaatt gttcacccta tcctgcgcaa tctaatggaa gagtctggcg aaacggtcaa
    14221 tatggcggtg cttgatcaaa gcgatcacga agcgattatt atcgaccagg tacagtgtac
    14281 ccacctgatg cgaatgtccg cgcctatcgg cggtaaattg ccgatgcacg cttccggtgc
    14341 gggtaaagcc tttttagccc aactgagcga agaacaggtg acgaagctgc tgcaccgcaa
    14401 agggttacat gcctataccc acgcaactct ggtgtctcct gtgcatttaa aagaagatct
    14461 cgcccaaacg cgcaaacggg gttattcatt tgacgatgag gaacatgcgc tggggctacg
    14521 ttgccttgca gcgtgtattt tcgatgagca ccgcgaaccg tttgccgcaa tttctatttc
    14581 cggaccgatt tcacgtatta ccgatgatcg cgtgaccgag tttggcgcga tggtgattaa
    14641 agcggcgaag gaagtgacgc tggcgtacgg tggaatgcgc tgactttttc tggcgggcag
    14701 aggcaatatt ctgcccatca tacctgagtg gcaatagaat aagggtgtct gttaatcgca
    14761 ttgacgccaa aataacttaa tgtcatacac ttcacacact tttattaact cagcattatt
    14821 tttaaacatc aaaccactta attattaaca aaatcgcaag atctcattcc accgaaactt
    14881 ttacggctgc attatgagat tgagcgattt atgatatttt tttagttgta atcatattaa
    14941 attttttcac tgttctgata gttaaaattc aagacatcaa taaacaatga gatatttaaa
    15001 tgattactcg tattcctcgt agttctttct ctgcaaatat taataataca gcccagacaa
    15061 atgaacacca aaccctgagt gaattgtttt ataaagaact cgaggataaa ttttctggca
    15121 aggagctggc gacgcctcta ttaaaaagct tctcagagaa ctgtagacaa aatgttcgtc
    15181 atatctttag caacaaggat tttgtcatta aattttccac gtccgtctta caagctgata
    15241 agaaagaaat tacgatgatt aataaaaacg aaaacacgac actcactcaa accattgcta
    15301 caatatttga agaataccta atggaaattt tacctcaacg ctcagacact cttgataaac
    15361 aagaattaaa ccaaaaatca gatagaaaag aaaaagaatt cccaagaatt aaacttaatg
    15421 gtcaatgtta tttcccgggg cgccccaaaa ccgtatagta taccgacaca ttgctgcaca
    15481 atatattaat gatatttatc agaatgttga ttacaaaccc catcaagatg attactcttc
    15541 agctgaaaaa tttctcactc acttcaacaa aaaatgcaaa aaccagactt tggcgttgat
    15601 ttccagccgt cctgaggggc gttgcgttgc tgcctgcggt gatttcgggc tagttatgaa
    15661 agcatatttt gacaagatgg aatcaaatgg catcagtgtt atggcagcca tattactggt
    15721 ggataaccat gctttgacgg tccggctaag aataaagaac acaactgaag gatgtaccca
    15781 ttacgtggtt tcggtttatg atcctaatgt aactaacgat aaaataagaa ttatgagcga
    15841 aagcaaagag gatattaaac actattctct gatggatttt atgaatgtag attatagcct
    15901 cctgaaatgg tcaaatgatc atgttattaa ccaatctgtt gcaataattc cagcacttcc
    15961 gaaagaacag ctattgatgt taaaaggaac tgtggatgaa ataacccctc cattatcacc
    16021 agcaacgatg aatttgctaa tggcaattgg tcagaatcac caacttacgc aactgatgat
    16081 tcagctccag aaaatgccag aactacatag aacagaaatg ttgactgcct ataatagtat
    16141 taacctcccc ggtttatatt tggctataaa ttatggcaat gcggatatcg ttgagactat
    16201 tttcaattca ttgtcagaaa caggatatga agggttactc tcgaaaaaaa atctcatgca
    16261 tattctggag gcaaaagata aaaatggttt ttctggatta tttttagcga tatcacgtaa
    16321 ggataaaaat gttgtaacct cgattctgaa cgtcttacct aaactggccg caacacatca
    16381 tttagataat gaacaggtgt ataaattcct gagtgccaaa aatagaacgt ccagccatgt
    16441 tttataccat gttatggcga atggtgatgc cgacatgctg aaaatttttt tggtcgcgtt
    16501 acctttgtta attcgcacat gtcatttgac taaagaacag gtactcgatc tcctgaaggc
    16561 aaaggatttt tatggttgcc caagactata cctggcgatg caaaatggac atagcgatat
    16621 cgtgaaagtt attctcgaag cattgccctg tctggcccag gaaattaaca tttcagcttc
    16681 cgatattgta gatcttctga ccgctaaaag tcttgcgcgc gacacgggtt tgtttatggc
    16741 catgcagcgc ggacatatga acgttattaa tactattttt aacgcattac ccactctttt
    16801 taatacgttt aaattcgata aaaaaatatg aagcccctcc tcctggcaaa taattctaat
    16861 gaatatcccg gtttgttttc agcgatacag cataaacaac aaaacgttgt agagacggtt
    16921 tatcttgctt tatctgacca tgcacgcctg tttggattta ccgctgaaga tattatggat
    16981 ttttggcaac acaaagcgcc acaaaaatac tctgcctttg agttggcttt tgaatttggt
    17041 caccgggtta ttgctgaatt aatccttaat acattaaata agatggctga aagctttggc
    17101 tttacggata accctcgata cattgcggag aaaaattata tggaagcttt actcaaaaaa
    17161 gcatctcccc ataccgtacg ctaaatcttt gcctgcgccg atacgcataa acatcttcca
    17221 catgcccttc acgtatgcgg ttttgtagtg cgcgccagta atcagcgcgg aacaggtcgg
    17281 cgtgcatctc ttcaaacagc ggaccaatac gcgggtcggc gcatagccag tggcgaaact
    17341 cttccgggaa aacatcgccc ggcgagacgc tgtaccacgg ttcgctggca agttcgtctt
    17401 ccggatagcg cggcggcggg atgtcgcgga aattcacttc cgtcatgtag caaatttcat
    17461 cgtaatcata aaaaaccaca cgcccgtgac gggtgacacc gaagttttta aacagcatgt
    17521 cgccagggaa aatgttagca gcggcaagct ggcgaatagc gttaccgtat tcttcaatgg
    17581 cgtcgcgcaa ctgctgacct tccacttgtt ccagccagat attgagcggc accatccgcc
    17641 gctcgattat agagatggcg aattacaatc tgctcaccaa gatcggtgat tttttccgct
    17701 gcttcctgga gcagtaattc cattaatacc ggggaaatat gccgcttctc cagcacaaag
    17761 ttttcaaact cctgggtgtc cgccattcgg cccacacggt cgtgctcttt caccagttga
    17821 tagcaggcac gaacgtgagc ggcagacatc tctttctgcg gcgcgaactt gtctttgatg
    17881 actttgaata cccgatcaaa gcccggcagc gtaaacacca acatcaccat tccacgaata
    17941 cccggcgctt caatgaactg ctcattacag ccctgtagat aaacgagata ttcgcggtag
    18001 ctttcggttt tggcgtgctt ctggcagccg atagccatat acaattcagc agtggtttta
    18061 cctggcagaa tttcccgtag ccactcgacc agcgctgcgg gcagcggtgc ataaaccata
    18121 aaataagaac gcgcaaagcc aaaaacaatg ctcgcttcgg cggtcgtcgt caggcaggta
    18181 tcaataaata actcgccgtc gtccgtctgg tggatcggca gtaagaaggg caacgtgccg
    18241 gaaggcgtga tcagtttgcc caccagccag gcggctttat tgcggtaaaa cagttcgttc
    18301 gccacctgta aatgactttc cgcgaggttg tctgtcccca gcgtttccgt cagatggcga
    18361 atgatgtaat gaatgtcacg gcttttattc tgccagcgca ggcgcagcgg caggtcgctg
    18421 ataacgcgca tcagtagaga ttcccagccg tgatcggggt gaaagtcttt cgccagcggg
    18481 cggggaatgg tacgaaagcg gcgctctggc tgagagctaa agataaaaag ccgctcggga
    18541 gtaagcgagc ggtggtcaaa taaccgacag tacacggagt taaaaaagct ctccgcaatc
    18601 tcgaagcgcg ggtaatccgg caacagccgc gtgtaatgct ctttaacgcg cagtaaaaat
    18661 gccgcgtccg tactttggcc gttagtaatg cagcgcagtt gctccacgac cagaccaacg
    18721 tgatgatcgt aaagatggat acggtttttc atcgcctgct ggacagcatg ccagtcggcc
    18781 tgttcgaaac gctgctgcgc cccggaagtc acttcgagga atcgaccata ctgagcgtcg
    18841 aagccttgca aaatggtttg agcaatcagt aattccaggc cacgcggcat ttagcgccct
    18901 catcaggagc agagaattgc cggatgcggc gtaaacgcct tatccagcct acgttcggca
    18961 acggttgtag gcatgataag acgcgcaagc gtcgcatcag gcatcgtgca ccgattgccg
    19021 gatgcggcgt aaatgcctta tccggcctac agtcagcaac gtttgtaggt tgcttagaac
    19081 tgcgattctt cagtggagcc agtcagcgcg gtgactgaag acgtgccgcc ctgaataata
    19141 gtcgtcactt tatcgaagta acctgtaccc acttcctgct ggtgagatac gaaggtatag
    19201 ccatctttcg cggcggcaaa ttccggctgc tgcactttct caacatagtg cttcatacct
    19261 tcgccctgcg cataagcgtt tgccaggtcg aacatgttaa accacatgct gtggatacct
    19321 gctagggtga tgaactggaa cttgtagccc atatccgaca gctgctgctg gaagctggca
    19381 atagttttgt cgtcgaggtt tttctgccag ttgaacgacg gcgagcagtt ataagccagc
    19441 agtttgcccg gatatttcgc gtggatagct tgtgcaaagc gacgcgccag ttccagatcc
    19501 ggcgtggagg tttcacacca gaccaggtca gcatatggcg catacgccag gccacggctg
    19561 atcgcttgct caatgcccgc atgagtacgg aagaagcctt cactggtacg ctcgccggta
    19621 ataaattcgc tgtcatacgg gtcgcaatct gaggtgatca gatccgccgc atcagcatcg
    19681 gtacgtgcaa ccagcagggt tggaacgccc gtcacgtcag ctgccagacg cgccgcgacc
    19741 agtttctgaa tagcttcctg agttggcact aaaactttgc cgcccatgtg accgcatttc
    19801 ttcactgacg ccagctgatc ttcgaagtga actgccgcag caccggcttc aatcatcgct
    19861 ttcatcagtt caaaggcatt caggacaccg ccaaaaccgg cttccgcatc ggcaacgatc
    19921 ggcaggaagt aatcgacata gcgcggatcg cccggctcaa tgcccacgga ccattggatc
    19981 tgatcggcac gacggaaggt gttgttgatc cgctccacca cagctggcac cgagtttgcc
    20041 ggatagagcg actgatccgg atacatgctg gccgccaggt tagcgtccgc cgctacctgc
    20101 catcccgaca gatagactgc ttcaataccc gctttcgcct gttgcagcgc ctgaccgcca
    20161 gtcagtgcac cgaggttgtt gatgtagcct tttttcgact caccgtgcag cagacgccac
    20221 attttcgctg cgcccagttg cgccagcgtg cattcaggat tgactgaacc gcgtaatttc
    20281 accacatctt ccgcactgta tgggcgagta atgccttccc aacgcggttg agtccactct
    20341 ttctgtaatt cttcaatttg ttgtgtacgg gttttcatgt gcagatgctc catagttatg
    20401 tggtggttta cgctaacagg cggtagcctg gcagggtcag gaaatcaatt aactcatcgg
    20461 aagtggtgat ctgttccatc aagcgtgcgg catcgtcaaa acggccctgg gagaaacgtt
    20521 cttcgcccag ttcgctggca atgactttca tctcttcgcc cagcatctgg cggaacaagg
    20581 ctttggtcac cggtttgcca ttgctcaacg ttttttgatg atggatccac tgccagatcg
    20641 aggtacggga aatttcagcc gtcgccgcat cttccatcag gccataaatc ggcacgcagc
    20701 cgttgccgga gatccacgct tcgatgtact gcacagccac gcgaatgtta gcgcgcatac
    20761 cttcttcggt gcgttcacca tcacaaggtg ccagcagctg atcggcagta atcggcgcgt
    20821 cttgttcgcg caatacttca agctgatttt tacgggagcc gagaatgtcg ttgaataccg
    20881 ccattgccgt gtccgcaagg cctgggtgag cgatccatgt gccatcgtga ccgttattgg
    20941 cttccagcgc tttatccgct tttactttgt tgagcacctg gttattacgc tcttcatctt
    21001 tgctcggaat aaacgcggcc atgccgccca tcgcaaaagc accgcgttta tggcaggttt
    21061 taatcagcag gcgtgagtaa gcattcagga atggtttatc catcgtcacg gcctgtctgt
    21121 ctggcaggac acgatcggga tagtttttca acgttttgat atagctgaag atgtaatccc
    21181 agcgaccgca gttcagacca acaatatggt cgcgcagcgc gtgaaggatt tcgtccatct
    21241 ggaacacggc gggcagcgtt tcaatcagca acgtcgcctt gatggtgccg cgcggcagat
    21301 taaagcgatc ttctgcatag ctgaagactt cgctccacca ggccgcttcc tgctaggact
    21361 gggttttcgg cagatagaaa tagggaccgc tgccctttgc caacagtgcc tgatagttgt
    21421 ggaagaaata gagcgcaaaa tcaaacaggc taccggggat tgcctcacca cgccaggtga
    21481 catgtttttc cggcaagtgc agaccgcgta cccgacaaat caaaaccgct ggattgggct
    21541 tgagctgata aattttgcct gcttcattgg tatagctgat ggtaccgtta accgcatcgc
    21601 gcaggttaat ttgcccgtcg atcactttgt tccagtctgg tgccagtgaa tcttcgaaat
    21661 cggccataaa gactttcaca ttggcgttca gtgcgttgat caccatcttg cgctctaccg
    21721 ggccagttat ctctacgcgg cggtcttcta agtccgcagg aatcccgcga attttccaat
    21781 cagcatcgcg aatggaagcc gtttccgaaa taaaatcagg caacgttccg ttatcaatat
    21841 cttgctgctg ctgaatgcgc gctgccagaa gtttattgcg ttgtggcgta aaatgcgtca
    21901 ccagctcagt cagaaattct accgcttcgg cagtaagaat ttgcttctcc tgctcgccat
    21961 acggccttgt gaaagccagt tcatcggttg ttgttgcctg ttcagtcatc gtgcagctcc
    22021 tcgtcatgga tccgaaaact tcccctctgt gaacgaagga acgttgtgcg cttttcgttc
    22081 agcacaacta agactactca tttaaaatgc aaaatcaaaa acaatttcca ttttaattaa
    22141 aaatacttga taacttattg ataacaatat gaataaaatt tattcacttg ctgaggtgcg
    22201 tttcggaaat aaaaaaggca cccgaaggtg cctgaggtaa ggtgctgaat cgcttaacga
    22261 tcgactatca cagaagatta atccagcgtt ggattcatgt gccgtagatc gtatggcgtg
    22321 atctgataga cgtaatagtt gagccagttg gtaaacagca aattaccgtg actacgccag
    22381 ctcgctcgcg gtttgttttg cggatcgttg tgcgggaaat agttatacgg tacatccggg
    22441 tctagtccgg cttccacatc gcgtaaatat tcctgcgcca gcgtttgcgc atcatattcg
    22501 ggatggcccg tcacaaaggc aatgcgctta tctttgctgg caaacagata tgcatcccct
    22561 tcttccgtct ctgccagaat ttccagatcg gtgtaatcac gaatcaacgc tgccggaaag
    22621 tcagcatagc gcgaatgcgg tgccaggaat gaatcatcaa agccacgcgt cagaagcgca
    22681 tgaggatgga gaatatgatg ctcgtaaacg ccagagagtt tgtcggtgcg agtttgctta
    22741 ggaatgccgt agaggatatt gagcgcggcc tgtaccgccc agcagacaaa cagcgtcgag
    22801 gtgacgtgat ctttcgacca ctccagcacc tgtttgatct gcggccagta agcgacatca
    22861 ttaaactcca ccaggcccag cggcgcaccg gttacaatca gaccgtcaaa gttctgttcc
    22921 tgaatatctt caaagttaca gtagaagttg ttcagatgct ctgcgggcgt gttgcgcgat
    22981 tcacgggaat cgatgcgcaa cagctgaata tcgacctgca aaggtgagtt tgaaagcagg
    23041 cgcagaaact gattttcagt ttcaatcttc ttcggcatca ggttaaggat cagaacctta
    23101 agtggacgaa tttcctgacc agacgcacga gaagttgtca tcacaaagac gttttcttca
    23161 cgcaagaaat tgacggcggg tagctcgtcc ggcacacgaa tcggcataac ctgattacct
    23221 cactacatac gcttatacgt ttagacatcc agatagctga agataaccag aaaatttgcc
    23281 aatgtcgagc ctgcatgttg aaggtgagaa agtttcaagg aaaaggctac gttagaatat
    23341 aagaatgacg aaaaggagag aaaatggtta ttagtattcg ccgctcacgg catgaggaag
    23401 gggaggaact cgttgcgatt tggtgtcgat ctgtcgatgc cacccacgat tttttatcag
    23461 cagagtatcg ggccgagctg gaagagctgg tgcgttcctt tctgccggaa gcaccgttgt
    23521 gggtcgcggt taatgaacgg gagcagccgg ttgggtttat gttgttaagt gggcagcata
    23581 tggatgcgct gtttatcgat cctgatgtgt gcggctgcgg cgtgggtcgg atgctggtgg
    23641 agcatgcgct ctcgatggca ccggaactga caaccaacgt taatgagcaa aatgagcagg
    23701 cggttgggtt ctataagaag gtgggtttta aggttacggg acgctctgag gtggacgatt
    23761 tggggaaacc gtatccgttg ctgaatctgg cgtatgtggg ggaataagcc gcctctgccg
    23821 tgcctgccca agctggagaa gacg
//
LOCUS       DAJTQQ010000059        22661 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000059 DAJTQQ010000000
VERSION     DAJTQQ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22661
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.059"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..567
                     /gene="exuR"
                     /locus_tag="OO309_002527"
     CDS             <1..567
                     /gene="exuR"
                     /locus_tag="OO309_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR5847634.1"
                     /translation="HVVSNQPRHQQAADNNMEFANYGPFELLQARQLIESNIAEFAAT
                     QVTKQDIMKLMAIQEQARGEQCFRDSEWGLQFHIQVALATQNSALAAIVEKMWTQRSH
                     NPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHLENTKIMLFNETS
                     DDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            912..1574
                     /locus_tag="OO309_002528"
     CDS             912..1574
                     /locus_tag="OO309_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5847635.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1578..1961
                     /gene="mzrA"
                     /locus_tag="OO309_002529"
     CDS             1578..1961
                     /gene="mzrA"
                     /locus_tag="OO309_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR5847636.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2108..2462
                     /locus_tag="OO309_002530"
                     /pseudo
     CDS             2108..2462
                     /locus_tag="OO309_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2500..2805
                     /locus_tag="OO309_002531"
     CDS             2500..2805
                     /locus_tag="OO309_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR5847637.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            2808..3212
                     /locus_tag="OO309_002532"
     CDS             2808..3212
                     /locus_tag="OO309_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR5847638.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3202..3501
                     /locus_tag="OO309_002533"
     CDS             3202..3501
                     /locus_tag="OO309_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR5847639.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3687..3983
                     /locus_tag="OO309_002534"
     CDS             3687..3983
                     /locus_tag="OO309_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5847640.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4053..5039
                     /gene="yqjG"
                     /locus_tag="OO309_002535"
     CDS             4053..5039
                     /gene="yqjG"
                     /locus_tag="OO309_002535"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR5847641.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5332..5700
                     /locus_tag="OO309_002536"
     CDS             5332..5700
                     /locus_tag="OO309_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5847642.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(5907..6803)
                     /gene="yhaJ"
                     /locus_tag="OO309_002537"
     CDS             complement(5907..6803)
                     /gene="yhaJ"
                     /locus_tag="OO309_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR5847643.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            6908..7609
                     /locus_tag="OO309_002538"
     CDS             6908..7609
                     /locus_tag="OO309_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR5847644.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7632..7796
                     /locus_tag="OO309_002539"
     CDS             7632..7796
                     /locus_tag="OO309_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5847645.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8008..9318)
                     /locus_tag="OO309_002540"
     CDS             complement(8008..9318)
                     /locus_tag="OO309_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR5847646.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9346..10677)
                     /locus_tag="OO309_002541"
     CDS             complement(9346..10677)
                     /locus_tag="OO309_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR5847647.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(10952..12316)
                     /gene="tdcG"
                     /locus_tag="OO309_002542"
     CDS             complement(10952..12316)
                     /gene="tdcG"
                     /locus_tag="OO309_002542"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5847648.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12388..12777)
                     /locus_tag="OO309_002543"
     CDS             complement(12388..12777)
                     /locus_tag="OO309_002543"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR5847649.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(12791..15085)
                     /gene="tdcE"
                     /locus_tag="OO309_002544"
     CDS             complement(12791..15085)
                     /gene="tdcE"
                     /locus_tag="OO309_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR5847650.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15119..16327)
                     /gene="tdcD"
                     /locus_tag="OO309_002545"
     CDS             complement(15119..16327)
                     /gene="tdcD"
                     /locus_tag="OO309_002545"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR5847651.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16353..17684)
                     /gene="tdcC"
                     /locus_tag="OO309_002546"
     CDS             complement(16353..17684)
                     /gene="tdcC"
                     /locus_tag="OO309_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR5847652.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(17706..18695)
                     /gene="tdcB"
                     /locus_tag="OO309_002547"
     CDS             complement(17706..18695)
                     /gene="tdcB"
                     /locus_tag="OO309_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR5847653.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(18794..19732)
                     /gene="tdcA"
                     /locus_tag="OO309_002548"
     CDS             complement(18794..19732)
                     /gene="tdcA"
                     /locus_tag="OO309_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR5847654.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            19922..20266
                     /gene="tdcR"
                     /locus_tag="OO309_002549"
     CDS             19922..20266
                     /gene="tdcR"
                     /locus_tag="OO309_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR5847655.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            20522..21062
                     /locus_tag="OO309_002550"
                     /pseudo
     CDS             20522..21062
                     /locus_tag="OO309_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21084..21431
                     /locus_tag="OO309_002551"
     CDS             21084..21431
                     /locus_tag="OO309_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5847656.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            21600..22250
                     /locus_tag="OO309_002552"
     CDS             21600..22250
                     /locus_tag="OO309_002552"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5847657.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKTYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
BASE COUNT         6108 a         5723 c         5218 g         5612 t
ORIGIN      
        1 catgtggttt ccaaccagcc gcgtcatcag caggcggctg acaataatat ggaattcgcc
       61 aattacggtc cgtttgagtt gcttcaggct cgccagctca tcgaaagtaa tattgccgag
      121 ttcgcggcaa ctcaggtaac gaaacaagac atcatgaaac tgatggccat tcaggaacag
      181 gcgcgcggcg aacaatgctt ccgtgattcc gagtggggtt tacagttcca cattcaggta
      241 gccctggcga cgcagaactc cgccctggcg gccatcgttg aaaaaatgtg gacccagcgt
      301 agccataacc cgtactggaa aaaactacac gaacacattg attcccgtac cgttgataac
      361 tggtgtgatg accacgatca aattctcaag gcgttaattc gcaaagatcc tcatgctgct
      421 aagctggcaa tgtggcagca tctggaaaac accaaaatca tgttatttaa cgaaaccagc
      481 gacgatttcg agttcaatgc cgaccgctat ctgttcgccg aaaacccggt agtgcatctc
      541 gatacggcca ccagcggcag caaatgaaat tcctgtccga caggcgctgc aatgcgcctg
      601 tttgtctcgt cggtaagcga aaagtataaa gtgtcagcct gtgtaaatcc tctcgccacc
      661 ctcccctgca atcagcaaaa tcagactcca cggacatgta attttgataa cgaacaacgt
      721 tgacctttgt tacaattaga ttcaatttga atttatgctt ttgaatgctt tcttatctca
      781 cgttttaaca gggaatagtt caggctgtgt tgatgtatca aacccgcaga acataccaaa
      841 acagcaataa cattgcggta gtgcatcttt taaaaccagc gtggcgttaa ccgattcacc
      901 aggaataatg aatggaactt ttgacccaat tgctgcaagc cctgtgggcg caggattttg
      961 aaaccctggc caatccatcg atgattggca tgttgtattt tgtcttgttt gtaattttgt
     1021 tccttgaaaa tggcttgctt ccggcggcct ttttaccggg cgacagttta ctggtattgg
     1081 tcggcgtgtt gattgcgaaa ggcgcgatgg gttacccgca aacgattctg ctgctgaccg
     1141 ttgccgccag cctcggttgc tgggtcagct atattcaggg gcgatggctg ggcaataccc
     1201 gcaccgtaca aaactggcta tctcatttac ccgcgcatta tcatcaacgc gcacaccatc
     1261 tttttcataa acacggttta tcggcgctgt taattggtcg ctttattgca tttgtcagaa
     1321 cactgctgcc gacgattgcc gggttatcag ggctgaataa cgcgcgcttt cagttcttca
     1381 actggatgag cggtctgctg tgggtattga tcctgacaac tctgggttac atgctcggca
     1441 aaacgccggt atttttaaag tacgaggacc agctgatgtc atgcctgatg ctgctcccgg
     1501 tggtgctgct ggtgtttggc ctggcaggtt ctctggtcgt gttatggaaa aagaaatatg
     1561 gaaatcgggg gtaagggatg caaatacctc gcatgtcgct tcgccagcta gcctggtccg
     1621 gcactgtttt acttctggtc ggcacgctgt tgctggcctg gtccgcggtt cgccagcaag
     1681 agtctacgct ggcgattcgt gccgttcatc aaggcacaac gatgccagac ggtttttcga
     1741 tctggcatca ccttgacgct catggcattc ctttcaaaag tatcaccccc aaaaacgaca
     1801 ctctgttaat tacatttgac tccagcgacc agagcgccgc cgcaaaagcg gtcctcgaca
     1861 gaacattgcc ccatggctac atcattgcgc agcaggacaa taacagtcag gctatgcagt
     1921 ggctgacccg gttacgggat aactctcatc gcttcggata acttcctgtt atccgaaaca
     1981 tatcactcac tttggtgatt tcaccgtaac tgtctatgat taatgaggcg gtggttaaca
     2041 ctaccgcttc gttgtctgga caatcgttcc tttgtaatag gtccgaatca caatggaagg
     2101 ttcaagaatg aaataccgca tcgctttagc tgtttctctc tttgctctta gtgccggtag
     2161 ttatgccact accctgtgtc aggaaaagga gcaaaatatc cttaaggaga tcagctatgc
     2221 cgaaaaacac caaaaccaga atcgtattga cggtctgaat aaagccctga gtgaagtccg
     2281 ggccaactgt tcagatagcc agctgcgtgc cgatcatcac aagaaaatcg caaagcagaa
     2341 agatgaggtg gcggaacgcc agcaagattt agccgaggcg aagcaaaaag gcgatgccga
     2401 tagaacggaa actggcagaa gcgcaggaag agctgaaaaa gctggaagcg cgcgactact
     2461 aactcacaat agtcactact tactcacctg gagaaaacta tgtcgaaaga acacactacg
     2521 gaacatctgc gtgctgagtt gaaatccctt tccgatacgc tggaagaggt gcttagctca
     2581 tctggcgaga agtcgaaaga agagttgagt aagattcgta gcaaagcgga gcaggcactg
     2641 aaacagagcc gttatcgcct gggtgaaacc ggtgatgcca tcgccaaaca aacccgtgtc
     2701 gcggcggcgc gtgctgatga gtatgtgcgt gaaaatccgt ggaccggcgt gggaattggc
     2761 gctgcaatcg gtgtagtgct cggcgttctg ctgtcgcgtc gttaattatg gcggacactc
     2821 atcacgcgca agggcccggt aaaagcgttc tgggcatcgg gcagcgaatt gtttccatca
     2881 tggttgaaat ggtggaaaca cgtctgcggc tggcggtggt ggagctggaa gaggaaaaag
     2941 ctaatctctt tcaactttta ctgatgctgg gcctgacgat gcttttcgct gcatttggtc
     3001 ttatgagcct gatggtgcta attatttggg cggttgatcc gcaatatcgc ctgaatgcga
     3061 tgattgccac caccgtggtg ttgctgctac tggcactgat tggcggtatc tggacgttac
     3121 gtaaatcgcg taaatccacc ttgctgcgcc atacacgcca tgagctggca aacgatcggc
     3181 aactgctcga ggaggagtcc cgtgagcagt aaagtcgaac gtgaacgacg taaggcgcaa
     3241 ctgcttagcc agatccagca acaacggctg gatatttccg ccagtagtcg tgaatggctg
     3301 gaggcaacag gcgcttacga tcgtcgctgg aatatgctgc taagtctgcg ctcctgggcg
     3361 ctggttggca gtagagtgat ggcgatctgg acgattcgcc atcctaatat gctggtccgc
     3421 tgggccagac gcggttttgg cgtatggagc gcctggcgtc tggttaaaac gaccctcaag
     3481 cagcaacagc ttcgcggtta acttccctct ggccggagca attccggcct tatccatcaa
     3541 attttttgaa tatttttgac agttttcctt gctaacaatc atcattcacc acgtttatga
     3601 ttctctccat cgacagcaac gacgctaata gcgcgccctt gcacaaaaaa acaatcagca
     3661 gcctgagtgg cgcagtggag agtatgatga aaaaattaga agatgttggt gtactggtag
     3721 cgcacatttt aatgccgatt ctgtttatta ccgcaggctg gggaaaaatc accgcttact
     3781 ctggtaccca acaatatatg gaagcaatgg gtgtcccggg ttttatgctg ctgacggcat
     3841 ttttatttca cagcaacttt gctgaaggcg tcaactcgct gatgttcatg aaaaacctga
     3901 caatttctgg cggattcctg ctgctggcaa ttaccggtcc gggcgcgtat agcatcggcc
     3961 gcctgctgaa taaaaagtgg taagcacgcg ctatacttaa cgataaaaaa cgaggaggaa
     4021 gctcctcgtt tttgctattg gaggagagaa aaatgggtca actgattgac ggcgtctggc
     4081 atgacacctg gtacgatacc aaatctaccg gcggtaaatt tcaacgttca gcttccgcat
     4141 ttcgtaactg gctcactgcc gatggcgcac ctggccccac tggcacaggc ggttttatcg
     4201 cagagaaaga tcgttatcat ctctatgttt cactcgcctg cccgtgggcg caccgcacgc
     4261 tgatcatgcg caaactcaaa ggactggaac cgtttatttc cgtttccgta gtgaacccgc
     4321 tgatgctgga aaacggctgg acctttgatg acagttttcc gggagcaacc ggagatacgc
     4381 tctatcaaca tgaatttctg tatcagcttt atctccacgc cgatccacac tacagcggac
     4441 gagttactgt tcccgtgctg tgggacaaaa agaaccacac catcgtcagc aacgaatcag
     4501 cagaaatcat ccgcatgttc aataccgcgt ttgatgcgct gggcgcaaaa gcgggtgatt
     4561 actacccacc agccctgcaa acgaaaattg acgaacttaa cggctggatt tatgacaccg
     4621 ttaacaacgg cgtgtataaa gctggttttg ccaccagcca gcaagcctac gacgaggcgg
     4681 tggcgaaagt gtttgaatcg ctggcgcgac tggagcagat tttaggccag catcgttacc
     4741 tgaccggcaa ccagctaacc gaagccgata ttcgcctgtg gactacgctg gtgcgttttg
     4801 atccagtgta tgtgacccac ttcaagtgtg ataagcaccg catcagcgat tacctgaatc
     4861 tgtatggctt cctgcgcgat atctaccaga tgccggggat cgccgaaact gtcaatttcg
     4921 atcacatccg taatcattac ttccgcagcc ataagaccat caaccctacg gggattattt
     4981 caattggtcc gtggcaggat ctcgatgaac cgcatggacg agatgttcgc ttcggttaaa
     5041 aaaaggcacc ttacgaggtg cctctgataa ttagctaagt gcatgttgtt catgtcggat
     5101 gcggcgtgaa cgccttatca aacctacaaa gcgtttatat tcaatatgtt acaatgaccg
     5161 cgtaggccag ataagcgtag cgcatctggc gattttgcgc ttgccatcag tcttagcgcg
     5221 ccttcacggc actttattaa ttccttgaac gaatatttac tgccatttca ttcacgtcta
     5281 ttcttaattt gctgctttaa aaacaagtga ttgagcaaaa ttgaggcaaa aatggactgg
     5341 tatctgaaag tactaaaaaa ttatgtcggt ttccggggtc gtgcgcggcg caaagagtac
     5401 tggatgttta ttctggtcaa catcatcttt acgttcgtgc tggggctgct ggataaaatg
     5461 ttaggctggc aacgcgctgg tggcgaaggc atcctgacga taatctacgg tattctggtg
     5521 tttttaccgt ggtgggcggt tcagttccgc cgcctgcacg acaccgaccg ctcggcgtgg
     5581 tgggcactgc tattcttaat cccgtttatc ggctggctta ttatcatcgt ctttaactgc
     5641 caggcgggta cgccaggcga aaaccgcttt ggaccagatc caaaactgga acaagaataa
     5701 tgattaagca ccttgtaaaa ggtgcttcaa attgttgaca aagtgcgctt tgtttatgcc
     5761 ggatgcggcg taaacgcctt atccagccta caaattgcca aaattcaata tattgcagtg
     5821 actgcgtagg cctgataagc gtagcgcatc aggcagtttt gcgtttgtca gcagtcagaa
     5881 gcaccttgtg aaaggtgctt aatagattat tttccagcga acaactttgg aatttcccgc
     5941 agacaccagg atttcgcttc ccccatactg tcacgacgcc aggccataat aatatcgatc
     6001 tcgctggtcg attccgggct gacgacacgc aaccgccctt ccgcaatatc tttttcgacc
     6061 atcggatacg gcatcgtcgc cacgccaagc cccgccagta atgcctgacg tttatcttca
     6121 atcgtgctca ccgttaagcg cggctgtttg tccagcagct gtacggtcaa caccgggcgc
     6181 tcacgagcgg tatccgctac cgcaattcca cgatatttca cacgcgtcac ttcagataac
     6241 ggttccggct cctgatgaat cgggtgatca ggcgcggcaa cgtagacgtt cattaacgta
     6301 tagagcttgc gcgagttgat ctccgacgag gaacgaaaat gcatatccgg cgcgataacg
     6361 atatccgccc gcccctgctc cagccgttcc cacgcccccg ccagcacttc tgtgatgatt
     6421 gccagttggg tattggcttt tgccgccagt ttgtcgatta acgggaaaaa ggcaggtgtc
     6481 ggtaccagcg cttcggtcac aatggtgaga tgcgtttccc aaccgcgcgc gagagcttcc
     6541 gcatcagtag tcagtttatc tgcggcttcc agcaaaacgc gccctcgctc cagcaacatc
     6601 ctcccgacat tggtgaattt ggtgcgatgg cccgagcggt caaacagcac cacatccagc
     6661 tcttcttcca gtttttgcat ggtgtagcta agtgcggaag gcacgcgtcc cagctcatcc
     6721 gctgccgccg caaaactgcc ccggcgatcg atcgcatcca taacccgtag tgcttccagc
     6781 gttaatgccc tttctttggc catttcgttc tcattcagga aatttgaaca taccgggcag
     6841 aatatctggc taacaatgta gcgtccagcc ccttaccata aaaggaagta aagagaggtc
     6901 aagaattatg attactaccc gaactgccag gcagtgtgga caagcagact acggatggtt
     6961 gcaggcccgg tatacttttt cctttggaca ctacttcgac ccgaaattgt taggctatgc
     7021 ctccctgcgt gtgcttaacc aggaagtgct ggccccaggt gccgcctttc agccgcgaac
     7081 ctatcccaaa gtcgatattt taaatgtgat tctggatgga gaagcagagt atcgcgacag
     7141 cgaaggcaat catgttcagg ccagcgccgg agaggcgttg ctgctctcta cccagccggg
     7201 tgtcagctat agcgaacaca atctcagcaa agacaaaccg ttaacgcgaa tgcagctttg
     7261 gctggatacc tgcccgcagc gagagaatcc gctgattcaa aagctggcgc ttaatatgga
     7321 caagcagcaa ttaatcgcct cgccagaggg tgcgatggga agcctgcaat tacgccaaca
     7381 agtgtggctg caccatatcg tgctcgacaa aggcgaaagt gcgaatttcc agttgcatgg
     7441 gccacgcgcg tatttgcaat cgattcacgg gaaatttcat gcgcttacgc atcatgaaga
     7501 gaaagcggcg ctaacctgcg gtgatggggc gtttattcgt gacgaggcta acattacgct
     7561 ggttgccgat tccccactgc gcgctttgct gatagatttg cctgtctagt tgtttttaca
     7621 gggagatgat gatgagtaaa aaatcgacca aaaagcgcca gccagcgaag cccgtggtgg
     7681 cgaaagaacc tgctcgcacc gccaaaaatt ttggctatga agagatgttg agcgagctgg
     7741 aagctatcgt cgcggatgct gaaacgcgtt tagccgagga tgaagctacc gcgtaaagac
     7801 aggttgccgg atgcggcgta aacgccttat ccggcctaca aaacacaaca acatcaacaa
     7861 attaaacacc ccgtaggccg gataagatgc gccagcatcg catccggcat caggtacaaa
     7921 acacaacaac atcaacaagt tgagcacccc gtaggccgga taaggtgcgc cagcatcgca
     7981 tccggcacaa tactcaaaac ctggcgttta tctggccttg ctcgccataa tctcgataat
     8041 ctgccgatcc gtttgctgca tcgaatggct tgctaacgca cacaggttgg caatcgactg
     8101 ctcaacatca tgcgccacga tcccttcatt gccggtcacg gcggtatcat ccagcgccat
     8161 taacaccgct ttccacgcag ccgaagcact ggtcgaaacc ttcatcgcgc agctgttcga
     8221 cgcaccatcg caaatcatgc cgctgacatc gccgatcata ctgctgatcg ccatcgagat
     8281 agtttcataa cgcccatcca ccagccatgc catcccggcg gcggccccca ttgctgcggt
     8341 cgttgcggca caaagtgcag acaaacgcgg taactggtta tggatgtaaa ttgcgctcaa
     8401 atgagaaagc atcagcgcac gcgccagtcg ttcatcatcc gctccgaagt gttctgctac
     8461 aaccaccaca ggcattattg cagtgatccc ctggttaccc gagccggagt tactcatagc
     8521 cggaagcgta gcgccgccca tacgcgcatc ggatgccgcg ctggtacgaa tcacaatgga
     8581 tgaagagaga tctttcgcca gcaaaccgcg ctcgcactgt ttttccagcg tcgcgccaat
     8641 atgcagcccc cacttaccgc tcaaaccttc ctgcgataac gcacaattta gcttcgcgga
     8701 atcgagaata aagcggatcg ccgcaaatgg gacttcattg acgaacttca ggatctcagc
     8761 cagcgtcgtt ctggaaagca ccgtcagcgg cgactcttgc tcgccctctg ccacacacgc
     8821 ctggtgggta aacaccacac cattgtgcgt ctcaatatgc acaatgttgg tatgcccgcc
     8881 gacgatggtg acacacgccc acttctcacc gttccagact ttggcgcgtg agaagaggat
     8941 ttcattgcaa ggttcctgga tcttaacaga gactttcccc gccgccagca gtgctttggc
     9001 atcggtaatt gcctgcgcag ttgcgtcttt cagcacttcc agcccggcgt tggcatttcc
     9061 acctaacgcc cccagcgccg ccgcaatcgg cagccccacc attcccgtgc cgggaacggt
     9121 gacgcccaga ccgttcttca tcagatttgg cgaaacccag gcttctacac gttcaaccgg
     9181 accttccagt tctgccgcag caaccgccgc cgccagcgcc agtgaaatcg gttcagtaca
     9241 tcccagcgcc ggttttactt cctcctgaac ggcgaggatg taacgctgcc ataacggatt
     9301 taaagtcgaa tcaaacatat taaaaacctt aaaatttcag gtaaatcaag aaaatgccag
     9361 gaacagagaa acacaaagca acaaaccagt gacgataatc aggtacagag acatcccttt
     9421 gtatttgtgc aatgccggta ctttgtaaac cagccacgcc gggatcaggc accctaccat
     9481 gccgaaaatc gggctacaga tagaggtgaa gctcaacacc ggtgcgttca gtacgatggc
     9541 gctccaggcc agcaaaatgg cgaaaatcat gatgccgcgc tgaacaagat tttcgttaat
     9601 cttctcggca ggcatcttgc gacgcaggat gttcattacg atcccttgcg ttgcttcgcg
     9661 aaagcctaaa tagacgccaa agaatgcagt catgacagca aagatattga gaatgacgct
     9721 gaccactttc acccacgctg cgccgtcacc gctaataaac tgtgcggcaa tcgccagcgc
     9781 ggaaatattc tgctcgtagg cttttaccgc ttcgtcatgt cccatcgcca gcgtgaacga
     9841 cacggcgtag aaaaagacgg tgacaaacaa aatgccaaac gcgatattca ttgcccgcaa
     9901 tgctttatgc cgcgccactt caattgattt ttcccgcgag cgataagaga tcaccatcgg
     9961 gcttaacgtc tggataaaca gaatcgacgt cagggtaaac ggcagcgtaa taatggcgtt
    10021 tttcaccagc agccccagcg gcggtagcga accgacgtta tacagatgcc acattcccac
    10081 catcgacacg cccagcgcag cgactaccag cagcttggtc agcaccatgt cggtcgaaat
    10141 tttgaataac aatttctcgc cgcgtgaaga gatcgccacc agaatgcaaa tcagtaccag
    10201 accataaaag ggactgtctg acagcaaccc ttccgtcacg ccgaaggtat gcagatagga
    10261 agcactatcg ttggtgatgg cggtggaata gacgaacatc caaatcacca gcattacgaa
    10321 atagagcgca cctaacagga tgccccagtt tttacctaaa taaccgctaa tgacactcgg
    10381 gtaatcttta cattctggtg attctgccag cgtattaata aacaaccgct gaaacagata
    10441 cattgccggg taaccaatca ccgatgagag caaaaatacc cacaatccca tcaaaccgac
    10501 ctgcaccggg agaaaaacaa tccccgcgcc aatcgccatc ccgatactca taatcaccca
    10561 gccggtgtca gtactgtcga atttgatcgc ttctcgccac tcgctctcac tcattccagc
    10621 acgacccgcc ggggtcgaag cgtctgcaat gacgccttta ttcgatgcaa tttccataat
    10681 ttctcgctca atattttgta gggcttatta tttttttccg agccgcatca aggcgatatg
    10741 cggtctacgt ttttgcaggc gagttattag aataaagaaa tgatacgcgc gaggtcagag
    10801 aaaatcttca caatcaagac tttaaaataa cggagatgga taagaatttt ctactaaatt
    10861 aatcacaaga gaatattcat tctctatttg cggcagatca caaaaaaaag gtgcacattt
    10921 gtgcacccaa ggatgaaagc tgacagcaat gtcagccgca gaccacttta atggccagtc
    10981 ctccgcgtga tgtttcgcgg tatttatcgt tcatatcttt gccggtttca tacatcgtct
    11041 cgatcacttt atcgagtgaa acacgcggtg ccgaggtgcg gcgcatcgcc atccgcgcgg
    11101 cgtttactgc tttcacggca ttaatggcat tacgttcaat gcacgggatt tgtacctgtc
    11161 cggcaaccgg atcgcaggtc agcccaaggt tatgctccat cgcgatttcc gccgcattgc
    11221 atacctgcgc cggactgccg cccagtagtt cagttaaccc tgccgctgcc attgaacagg
    11281 ccacgccaat ctccccctga cagccgacct ccgcgccaga gatggaggcg ttcattttat
    11341 acagcgcgcc aatagccccc gctgccagaa aatagcgggc aattgaccgc tcgtttaccg
    11401 gacgacggaa cttatcgtaa taagccagca ctgccggaat aatgccgcac gcaccgttag
    11461 tcggtgccgt taccacgcgc ccgccagctg cgttttcttc actaaccgcc agcgcgtaca
    11521 tgttgatcca gtcgatgaca ttcatcggat cgttagagat gttatcgcta gaaaccagct
    11581 gacgacgcag cgctacggca cgacacggca cattgagcgg accaggcagc acgccttcag
    11641 tgttcatccc gcgttcaata ccgtcatgca tcacttgcca gatacgggca aaccctgcgt
    11701 caatttccgc tttgctgcgc agcgccagct cgttgtgcat catcaggcca gatatagaca
    11761 ggccgttgta atcacacatt ttcagcagtt cacctgctga gtggaaatcg taaggtacgg
    11821 acgtttcgac atcgtgcgac aggccgaagt gttcttcttc gacaataaac ccgccgccga
    11881 cagagtaata ggttttactt aatagcgctt cctgtccctt ccaggcagtg atccgcattc
    11941 cgttctcatg gcgaggcaac atttcgggat ggaagataat gttctttgct acaggaaaat
    12001 caacaatatg cgcaccagat gccactggca gccgcccgct gcgcgttact aactctataa
    12061 atgcagggat ctcatcaatg acaacatcct gcggactgtt tcctgccagt cccatgataa
    12121 tggcgacatc cgtggcatgg cctttgcccg tcagtgacaa cgacccgtac agatcgacca
    12181 caatatggct cgtcgcggtt aataagccgc tactttccag ccgatcaata aaactttttc
    12241 cggcattcat tggccccacg gtatgcgaac tggagggacc aatcccaatt ttgaaaatat
    12301 cgaatgcact aatcatatcc acaccctcgg attgccgttc agtgaagtgg agcggaacga
    12361 ccttgcgacc gtcccgctca cgaggcttta cgcactacgt actgcgatag cttcaatttc
    12421 cagcttcaca tctttcggca aacgcgcaac ctgcacacag ctccgggtcg ggtaggtcgc
    12481 ctgatgctca tcgaagaact gcttatagac ttcattgatg gtggcaaaat cattcagatc
    12541 ggtgatgaac acggtcatct tgatgatatc gcccacgctc agcccggcag caaccacgat
    12601 cgctttgacg ttttcgaggc ttaaacgcgc ctgatcttgc acatcagccg ggatctcacc
    12661 ggtctgcggg caaaccggaa tttgcccgga ggtgaagacc atgctgccta aatcaacgcc
    12721 ctgaacataa gggccgattg cgcctggggc acgttgcgtt tcgataatct ttttcataca
    12781 tcctccggcg tcagagcgcc tgggtaaaag tacgtgaaat aacatcctgt tgctgttcac
    12841 gggtcagtgc gttgaagcgc acggcgtagc cagagacacg gattgtcagg ttaggatatt
    12901 tttccgggtg ctcgatggca tccagcagca tttcccgatt cattacgttg acgttgaggt
    12961 gttgaccgcc ttcgacatcc gcttcgtggt ggaaataccc atccagcagg ccgacaaggt
    13021 tggttttacg tactggatct tctttgccca gcgccgcagg aacgattgag aaggtgtacg
    13081 agatcccatc tttggcgtag gtgaacggca gtttcgccac cgacgtcaat gaggccacgg
    13141 cacctttgcg gtcacgacca tgcatcgggt tagcgcccgg cgcgaacggt gttccggcgc
    13201 gacgaccgtc cggcgtatta ccggttttct ggccgtacac cacgttagaa gtgatagtca
    13261 gaatcgactg ggtagggacg gcgttgcgat aggttggcag cgctttaatt ttcttcataa
    13321 agcgttcaac caggtcgcag gcaatgctgt ctacgcgctc gtcgttgttg ccgtactgcg
    13381 gatattcacc gtcgatttca aagtccaccg ccaggccgtt ttcgtcacgg attggtttca
    13441 cgcgggcata tttgatggca gacagggagt ccgtcgccac cgacaggccc gcgatgccgc
    13501 atgccatagt gcgatagaca tcacgatcgt gcagcgccat cagcgaagct tcgtagctgt
    13561 acttgtcgtg catgtagtga atgatattca gcgcgctgat gtactgcacc gccagccagt
    13621 ccatgaagtg atcgaggctg tccatcactt tgtcgtaatc cagcacgtcg tccatcagcg
    13681 gtgctgtttt cggcccgacc tgaatcttca gcttctcgtc caccccgccg ttaattgcgt
    13741 agagcagcgt tttcgccagg ttagcgcgtg caccaaagaa ctgcatttgc ttaccaatca
    13801 ccattgggct gacgcagcag gcaatcgcgt aatcgtcgct gttgaagtca gtacgcatca
    13861 gatcgtcatt ttcatactgc aaggaagagg tgacgatcga cacctgcgcg gcatattttt
    13921 tgaaggcaat cggtaattct tccgaccaaa gaatggtcag gttaggttcc ggtgccggcc
    13981 ccatagtgtg cagggtgtgc aaatagcgga aggagttttt ggtcaccagc gtacgaccgt
    14041 ccagccccat cccgccgatc acttccgtcg cccagattgg gtcgccggag aacagcgaat
    14101 caaattccgg tgtacgcagg aagcgtacca tacggatctt catgatgaag tgatcgatca
    14161 gttcctgtgc ctgctgctca ttgaggacgc cagctttaaa gtcgcgctca atgtagatat
    14221 ccaggaacga tgccgtgcgc cccagcgaca tcgcgccgcc attttgcgat ttcactgccg
    14281 ccagataagc gaagtagagc cactgcaccg cttcctgtgc attctgcgcc gggcgagaga
    14341 tatcaaagcc atatttcgcc gccatttcct gaatctgcaa cagcgcatga cgatgctctg
    14401 ccagctcctc acgcagacgg atggtggctt ccagatcctc gcctttttcc agacgagact
    14461 ggagatcggc aaattgcagt tcgcgttcac gcaccagata actgatgcca tacagcgcta
    14521 cgcggcgata gtcaccgata atgcgcccac gaccatagcc atctggtaaa ccggtcagca
    14581 cgccagattt acggcagcgc agcatatccg gtgagtaaac atcaaatacg ccctggttat
    14641 gggttttacg cagatcggta aacagatatt caaattcact gtccatttca cggccatagg
    14701 cgtggaatga acttttaatc atattaatgc caccgaacgg gtgtagcgca cgtttcaacg
    14761 gcgcatccgt ttgcaggcca acaatttttt ccagcggctg gttaatatat cccgcatcat
    14821 gagcggtaat tgtggtggca atattggtat cgaaatcaac cggcgcgtgg gttgcgtttt
    14881 cgatacggat gccttccatt accttttccc acaattccgt ggtggcaggc gtcgcttcgg
    14941 cgagaaaaga ttcatcgcct tcatacggtg tatagttatg ttgaataaaa tcgcggacat
    15001 taatttcgct tttccagtcc gtacctttaa agccaagcca tgcgtcagcg tacagcttat
    15061 cgctggtatc aatatctacc ttcatgaaaa ataatctctc tacaatactt caactaaatt
    15121 atgcaaattc tgcgggcgcg ttaactttac ctaaatgaat ggcatccaaa gcaatcattt
    15181 tttcttcgtt agtcggaata acgacgcaaa tgacatgcgc attttcactg gaaacaattc
    15241 gctcgccaaa agagttagaa cgattattca tttctgtatc aatctctacg cctaatacag
    15301 ccaaatgttc catgaccaga cgacgaatta aacttgaatt ctctcctatt ccgccggtga
    15361 atataattcc atccagccga cgtaatgaag ctgcatgtcc ggcaatatga cgggcaattc
    15421 ggtgaacaaa ggttttaatt gccagttgcg cgcgttcgtg accttcatgc caggcttttt
    15481 ccagaacacg taaatccgaa gaaagaccgg aaatacctaa taatcccgac tctttattca
    15541 ctacgcgttc caggtcaccc aggctctggt tggtttggct ggcgacccag gacatcgcac
    15601 caaagtcgac atcgccactg cgggtaccca tcatcaagcc ttccagcggc gtcattccca
    15661 ttgaggtgtc aacactctga ccgttgcgaa ctgcgcagat tgacgcgcca ttgccaagat
    15721 gcgccacaac caggccggag tcatcttccg ccagattcag cagcgaatgt gcgcgctggg
    15781 aaacatagcg gtgcgacgtg ccgtggaaac cataacggcg tacacctaac tcttcataat
    15841 atttccacgg caggccgtat aaataagctt ccggagccat cgtctggtgg aaactggtat
    15901 caaataccgc cacctgagtt acgcccggaa ataattgctg cgccgattca ataccactta
    15961 aatttgcgta attatgcagg ggtgccagtg gagaaacgcg acggatatta tcaatgactt
    16021 catcggtaat aatggcggac tcggtaaaaa tactgccgcc gtgagcgatg cggtggccaa
    16081 ttaaggccac gctgtcattt aaattccgtt tttccagttc aaatgcaatt gccttcaatg
    16141 caccttcgta gctgtggtga gccagcggtg ctggctctcc cccatttacg gataagaatg
    16201 cattttccga gttaataccg tcggcaatac ctgacattaa tacttcacaa tcgctggcat
    16261 cgagtacgga aaacttaatc gaagacgaac cacagttaat aaccaaaaca accggaaatt
    16321 cattcatctc ttttctcatc ctgagttacg gattaaaaca gtttgtatac gatgttcagg
    16381 atggtcagca gaccaatcac ggtaacaaac acgttatcca gacgaccacg gtatttcgcc
    16441 agagacggcg ctttacggat agcatacatc ggcaacaggc acagcaggga tgcgataatc
    16501 ggtgcgccca tggcttcaat caggtcgagg atgttcgggt tggcgtaggc aacaacccag
    16561 gtggagccca tgatgaagat catgctgaga gtattcagtt tacccagcga cactttggtt
    16621 ttgtcacctt tataaccgaa cttcagaatc agaccattca agccttccag cgtccccaga
    16681 tagtgaccga agaaagattt gaagatagcc acgagtgcga tgatggaagc cgcatattcc
    16741 agtgtaatcg cgaacgttgt tttcgtaccg gtcatggacg caaagtggtt agccagataa
    16801 gaaagcactg gaatattctg cgctttggct tccgccatgt tggccggaga cagagtaaac
    16861 aggcagctaa aggcaaagaa catcaccact gcaaccatca gcatgctggc acgagaaatg
    16921 atttgggaac atttacgttc ggtgaagtcg cgaccgaagt ctttctcata ctcttcacgt
    16981 ttagaaacca cgaaggaaga gacgattggc gagaagttaa aggagaaaac catgatggaa
    17041 atccccagcc agacagtgat caggataccg tcatgaccgg ttaacgacag cgaaccgagg
    17101 tcaacctggt cgataactgc agagttccag taagggatca gcgacagaga aatcagcacc
    17161 aggctggcga taaacggcca taccaggtag ctcatcactt taaccatcag atccttacca
    17221 aaccagatga cgaaagccat cagcagcaac aggaacagcg ccacaaagcc gcgattcagc
    17281 ggtgcaaatc cgagctggtt ttcccagaac gtcataaagg tattggtaat agtaacgcca
    17341 taaatccaca gcagtgggca aatcgcgaag aagtacagga acgtgataac cacgccgcca
    17401 gttttaccaa aatgctcttc caccgtttcc gtaatgttgc cggaagggtt agagccagaa
    17461 agacacagac gcgccagcgc ccggtggcaa taaaatgcga tggggtatgc caataccaac
    17521 atcagaagaa tcgggatcag tccgccaaaa cctgcgcgga tagggaagaa cagcaccccg
    17581 gcgccgattg ccgtaccaaa caagcctaac gtccatgtgg tatctgattt acgccaggac
    17641 gattgttttg tctggctgga tacaatgcta tctgaagtac tcatatccta tcctcaacga
    17701 attaattaag cgtcaacgaa accggtgatt tgagagacgc gagaaagatc gatattgccg
    17761 ccggaaataa tactgacggt ttttctattt tgaatatatt ggtctaattt accgcttaat
    17821 aatgcagcac atgccagagc gcctgcgcct tcggtgacga ctttattgcg ctgaattaag
    17881 gcaatcatac tgtttctgat ttcgtcttcg ctgaccagca cgatgtcatc gactaattca
    17941 cgaacgattt cgtaagttaa attacccggg cgggagacat cacaaccatc cgccagggtg
    18001 ccggtagttc ggtgcgtggt tatttctccg gagtggaaag aagccgccat gccgtgaacg
    18061 ttttctgact gtacgccaat aacacgaatg gtcgggttaa tagatttaat tgccaccgca
    18121 ataccagcaa ttaaaccacc accaccaatt ggcacaatca cgttatcgac atcatagaga
    18181 tcttccataa tttccagacc aatcgttccc tggccagcaa tcactttcgg atcatcgtaa
    18241 ggtgggataa aaatacgacc ttccatttcg acaatttcgc tcactttagc gatagtgtcg
    18301 ttgaagttat caccatgcag aacgacttct gcggagtagt cgcacgttgc cgctactttg
    18361 gattttggcg caccttttgg catcaccact ttaccgtcga tacccagcat cgcgcaggag
    18421 agcgaaaccc cttgcgcatg gttgcccgca gaacaggcca ccacgccttt gcgtttttcc
    18481 gcatcggtca gtgaacttaa tttattaaat gcgccacgaa ttttaaatga acccgtacgc
    18541 tgcatatttt caaacttcag gaatatttca cctttgcaac gttcactaaa atagttggag
    18601 cgaggcatgc ctgttttata aattcgccca gccagtcgtt gtttcgcttc aataatatca
    18661 tcaatagcaa ccggcagatc gtatgtaata tgcattataa aacctcttcg cctgaattaa
    18721 atatgtaggt aaccgtaacc gacacctgca aaacagacag gtggattatt tatgtttaaa
    18781 taaaaacaaa caactaacca acttctatta attgccttcg tctacaccca ttataagatg
    18841 aatactcttt ggctaattcc accaaaaccg atgctgcttt tttaatacga taatttttcg
    18901 accataccgc ggcatattgt gccacaggta atgtttcttc aaccggaata gtaataaatt
    18961 gattagaacc aaaaggtgac gtcatatcac aaggaattac agttaagaaa tcagcattga
    19021 gaacaagatt ataaattgtc acgactgagt cggttttaac gatgttttca atactgatgc
    19081 catttctttg taacgtagta agcagttcgc tgtagtaccc catattagtt tgtggcaaca
    19141 cccactgttc gttcttcaac gactccagcg tggtggtgcc ggtgcatgtt cgggacttac
    19201 tggctaccag cacaaactcg gactcgaaca gcggctcaac atgtaaatcc tgaagcttca
    19261 tttctgcact taacgtacca atcgcaaaat ccagtcgacc gtcgcggatt gccggtaaga
    19321 acgaagacag ttgcgcttca tacatagaaa cctgcgcttt cgggaacacc tctttgaact
    19381 tgttgatcat ccctgacata aaagtaaaac caatcaatga aggaaaacca aatgagactt
    19441 ccaccaccgc ttcagaagac ataccgctta tctcattaac catatttttc atttcacggg
    19501 taatggattc ggaacgggag agtaacaatt gaccggcagg tgttaatgtt acaccggtat
    19561 ttttccgcac cactaattcc acaccaaaat aatcttcaat atcgttaatg attttactga
    19621 cggccggttg agttaaccct aattcttttg cagccgagcc gatagagcca cttctaatga
    19681 cttcctgaaa gactaccagg tgctgcgttt tcggaagaag aatagtgctc ataacgacct
    19741 acgttaatta cctcattgac ggcatgaagt gtatcaaaat gaaataaaca ggatatgtgc
    19801 gaccactcac aaattaactt tcattacttt ccaaggtatc gttattacaa ttaaacatct
    19861 ttaaatatca acaagttaaa gcataaaaat cagcataaac cctgattttt atcaaaaaaa
    19921 aatgacgggg ataaccatat tttatggcga taacatcatt cgttatgtgg ttaatacaaa
    19981 aaaggggctg agaccatatt tcaagcaatt accggataat taccaggcaa aatttgagtt
    20041 aaatttaatg agcaagttct caaattttat tataaataaa ccattttcag cgataaatac
    20101 tgcggcacgt cacatttttt cacgctattt gttggagaac aaacatttat tttatcaata
    20161 ttttaaaatt tcgaatacag gtattgatca tctcgaacaa ttgattaacg tcaacttttt
    20221 ctcttctgac aggacgtcat tttgtgaatg caatcgtttt ccataaattc ttctcccctc
    20281 ataggcgacg aatagcattt tgtgttgagg atcacaaaac gaataattgc tggtcgccgc
    20341 gataaggtca gacgaagtca acaaggggga ttttcactgt gctttacatc agcgtaggac
    20401 acagaatgtt gcttcgttta ctgtaacgcc gggtaaatga gcgttttttg atagtgcgta
    20461 agtgtcgtgc gaggaaaata agcatctatt attgtttgtc atgacgacaa ggcaggcatc
    20521 aatgaaaggc ttcccaattg cacatatttt tcacccttca atcccgccaa tgcacgcagt
    20581 ggttaacaat cacaatagaa atattgatta ttggacggta aaaagaaagt ttgcagaaat
    20641 tgtctccacc aatgacgtta ataaaattta cagtataagt aatgaactcc ggagagtatt
    20701 atctgcaatt actgcattga atttctatca aggcgatgtt ccttctgtca tgattccgaa
    20761 tccaaccgga aaatatgagt ccattcatca tagatatttc tacaggagaa catgatgatt
    20821 atatcataca aacattagat gtaggcactt ttgcaccttt tggtgaacaa tgtacttgct
    20881 cagccgtcaa taaaaaagag ctggaatgta ttaaggagac gatttctaag tattgcgcaa
    20941 agttcaccca aaaagaagcc atcttaacac cccctaggca ctttaataaa acctgcatta
    21001 cttcagattg ttggcaaatt ctcttttttt cacccgatca ttttaataat gatttttatt
    21061 gattatttat aaaggataaa ataatgttcc ctgtttcatc aattggtaat gatattagta
    21121 gtgatttagt ccgtcgaaaa atgaatgacc ttcctgaaag ccccatagga aataacctcg
    21181 aagcactagc accaggtata gaaaaactaa aacagacctc tattcaaatg gtcactttac
    21241 ttaacgcgtt acaaccaagt ggaaaatgca ttatcactgg tgattttcaa aaagaattag
    21301 cgtacttaca aaatgtaatt ctttataatg actcgtctct tcgtctggat tttttaggtt
    21361 ataacaccca aattattcaa cgatcggaca atacctgcga acttaccatt aatgaaccgt
    21421 taaaaaacta ggaaatatct acaggcaata tcaatgttaa ttgcccatta aaagatattt
    21481 acaatgaaat caggaggttg aacgtaatct ttagttgtgg gactggagat atagttgatc
    21541 tatcctctct ggacttacgt aatgtcgatt tagattatta tgatttcaca gataaacata
    21601 tggctaatac tattctaaat cattttaaat tggattcaac aaattttact aatgccaaca
    21661 tgtttcaggt taattttgtt agttcaacac aaaacgccac aatctcctgg gattatttac
    21721 taaaaataac gcctgtttta ataagcatta gcgatatgta ttctgaagaa aaaatcaagt
    21781 ttgtcgaaag ttgtttaaat gagcttggag acattaccga agaacaatta aaaattatga
    21841 gatttgcaat tataaaatct ataccaaggg caactcttac agataaatta gaaaatgaat
    21901 taacaaaaga aatatataaa agctcatcga aaatcaacaa ttgcttgaac agaattaaat
    21961 taccagagat gaaagaattc tcatcaaaaa aaacatatga ttacatcgat acaatcattg
    22021 aagattatga aaatatcaaa gaaaatgctt atctggtcgt cccccaaatt aattatacta
    22081 tggatttaaa catagaagac tctagctcag aagagttaat accctcgaga aagacgaaaa
    22141 ttctccggac aatggctttg aggtcgggga atataacaca tatgaagcat ataactcaga
    22201 gaatcaatat tttaccagag aggactatac gtataattac gaccttttaa atgcaatata
    22261 gacttaataa cttcttacaa agtctattat taataccgta gatattcatc ttaccctgtg
    22321 caccaaaagc aaaaacttat cgacgcgggt ttgaaactga tggcaaccgc aaaaatgcct
    22381 gatgcgctac gcttatcagg cctacgccat ctctgcaata tattgaattt gcgtgctttt
    22441 gtaggcagga taaggcgttc acgcagcatc cggcatgaat aaagcgcact caacaatctg
    22501 aaacccgccg gaacggttta ttacgtacat caggtgaaac tgaccgataa gccgctttct
    22561 tttgggtata gtgtcgtgga cagtcattca tctttctgcc cctccaaaag caaaaacccg
    22621 ccgaagcggg tttttacgta tatcaggtga aactgaccga t
//
LOCUS       DAJTQQ010000060        22035 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000060 DAJTQQ010000000
VERSION     DAJTQQ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.060"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..782)
                     /locus_tag="OO309_002553"
     CDS             complement(<1..782)
                     /locus_tag="OO309_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5847658.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYG"
     gene            complement(884..1864)
                     /locus_tag="OO309_002554"
     CDS             complement(884..1864)
                     /locus_tag="OO309_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR5847659.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1872..2582)
                     /locus_tag="OO309_002555"
     CDS             complement(1872..2582)
                     /locus_tag="OO309_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR5847660.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(2799..4622)
                     /gene="typA"
                     /locus_tag="OO309_002556"
     CDS             complement(2799..4622)
                     /gene="typA"
                     /locus_tag="OO309_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR5847661.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4995..6404
                     /gene="glnA"
                     /locus_tag="OO309_002557"
     CDS             4995..6404
                     /gene="glnA"
                     /locus_tag="OO309_002557"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR5847662.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6577..7626
                     /gene="glnL"
                     /locus_tag="OO309_002558"
     CDS             6577..7626
                     /gene="glnL"
                     /locus_tag="OO309_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR5847663.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7638..9047
                     /gene="glnG"
                     /locus_tag="OO309_002559"
     CDS             7638..9047
                     /gene="glnG"
                     /locus_tag="OO309_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR5847664.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9159..9269
                     /locus_tag="OO309_002560"
     CDS             9159..9269
                     /locus_tag="OO309_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR5847665.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9498..10871)
                     /gene="hemN"
                     /locus_tag="OO309_002561"
     CDS             complement(9498..10871)
                     /gene="hemN"
                     /locus_tag="OO309_002561"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR5847666.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11060..11569)
                     /gene="yihI"
                     /locus_tag="OO309_002562"
     CDS             complement(11060..11569)
                     /gene="yihI"
                     /locus_tag="OO309_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR5847667.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDECLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            12151..12783
                     /locus_tag="OO309_002563"
     CDS             12151..12783
                     /locus_tag="OO309_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR5847668.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13165..15951)
                     /gene="polA"
                     /locus_tag="OO309_002564"
     CDS             complement(13165..15951)
                     /gene="polA"
                     /locus_tag="OO309_002564"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR5847669.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16315..17247
                     /locus_tag="OO309_002565"
     CDS             16315..17247
                     /locus_tag="OO309_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5847670.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17288..18718)
                     /locus_tag="OO309_002566"
     CDS             complement(17288..18718)
                     /locus_tag="OO309_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5847671.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYSPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(18873..19499)
                     /gene="dsbA"
                     /locus_tag="OO309_002567"
     CDS             complement(18873..19499)
                     /gene="dsbA"
                     /locus_tag="OO309_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR5847672.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19516..20502)
                     /gene="srkA"
                     /locus_tag="OO309_002568"
     CDS             complement(19516..20502)
                     /gene="srkA"
                     /locus_tag="OO309_002568"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR5847673.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20579..20848)
                     /locus_tag="OO309_002569"
     CDS             complement(20579..20848)
                     /locus_tag="OO309_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR5847674.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            20918..21502
                     /gene="mobA"
                     /locus_tag="OO309_002570"
     CDS             20918..21502
                     /gene="mobA"
                     /locus_tag="OO309_002570"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR5847675.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21484..22011
                     /gene="mobB"
                     /locus_tag="OO309_002571"
     CDS             21484..22011
                     /gene="mobB"
                     /locus_tag="OO309_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR5847676.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5443 a         5524 c         5280 g         5788 t
ORIGIN      
        1 atgccgtaag cccccaccag cgcaacgggc aatagataga tgtttttcag gaatggaatg
       61 aagaatgtca ggccacagta aaccgcgtaa acgaagaaga cgttgaaagc gataagccag
      121 atggttttgt ctctcagcac tgaggtcata ctggtgtttt gcgatgctcc gtcttctttt
      181 ttcacctcaa cggacggtgc ctcttctttg tcattcagga caaagaaaat aataatgcct
      241 acggcaatca caataaggga atagaaccag atgcctgctt taaaacctaa taagccactg
      301 ccaaaccagg taaatactgc caacgcagaa aatgccacca cggtatcgac aatgccacgc
      361 cctgtttcga agaagccaaa caaccgacct tgttgttcgc tattgcccaa tcgacttacc
      421 gacttgagca agaccggcca gttcatcatg tcgcaagtaa cgccaaatag cgcccagaca
      481 aagaggatgc cccaataccc cggcattgtc gtcaaataaa cacccagtaa tcctgtcgca
      541 atgagtgaga atgacatggt gtatctgcgc ggtagtttat cggcaaaata aatagacaga
      601 aaaaagccca ctgtggtgac aaatgagttt accgacatag cattaccaat ttgaccattg
      661 gtcaaatgga aatattcctg catcgggata taaaacgcat ctttcagcga cggtaattta
      721 taaattgtac cgccacacag tgttaataga ctaaataacg cccatttctt tttcgtgagc
      781 ataacatccc ctaagcgatt tttaaaatca ctgttttaaa ggcaataaga taccgaccat
      841 tcatatggaa tggttttatt aactgcgtat gagttaaaac ttattagtgg ttcagaagat
      901 ggtttgcctc ttgtttgatt tgttgtagta cgttacgcac atgatttatc tgattaattg
      961 catcttcttt ttctttacgt aaccgcgcat ccagtaaatg attttctggt aggggtgttt
     1021 cactcatctg gcgataaggg atccacggat tatcatcttc gtcttcaaag cggaacgccg
     1081 acgcatagta atcaacctct tcttccaggc catatgccgt cacctgcggc tcatcatcgc
     1141 caaggcagat aaggtgcgtt aataacgctt tgaatggcat atcaccctga cctgaaatac
     1201 aggctttatg acccaggcca ccctgttctt taacgatcaa ggcatcttta atatggacct
     1261 gggtaatatg cggtgccatc gtttttaagg cgtcgatggg atgctcattt gcattgatca
     1321 tgttagcaaa atcaaacagt aaggaaagag actccatctc actttctttg accagcgaca
     1381 ccagctcatg actttttaaa tcttcatgct gctcaatcgt aaaagtcagg ccgctgtcct
     1441 gatacgtttc ccgtacatag gcaatgtcgt tagcgataat cgataatacg tcgcgcaaat
     1501 taccttcata acatggataa aaacgtacgg acgttgcccc agttttcaac gcgatggcga
     1561 cggcttcgtc aatagatgcc ttatctgaag cgctggtttc aatatgaata tcaaggttca
     1621 gacggcgggc tttatcccca aaggcagaga gttctttatc gtccatatta ccaagagaaa
     1681 aacgctcgcc atccagaaca tggattttga cgccacgtag attattttca ctggcgatat
     1741 caagtaaatc agcaggcaac actctttcca gccgcatatt taaatgaaag gcataagcat
     1801 gtaaataaag aggaagagtg tcgacacgtt gtagaatctt tcttgcgtta tttattgtca
     1861 ccataaaaca cctacagatt aatgatgagg tctacggcct caaatcgcca atattcaata
     1921 tctatctgga caatattgcc ttgctggtcg gcgcgatgct tttcaattaa gatcgctggc
     1981 atacccgcag aaccgccaag atacttgctg atatcgcccg gcattctgac gggtttgaaa
     2041 atcaattttt ttactaccgt ctctttttgg tagcactttt cccagactgc agaaaatgag
     2101 tggttttcaa gttgttcaat aaaacccgga gcaacttcag gattaatata tgtttcgtga
     2161 tagaaaactt tatgtccttc cagcgccccc cagccggtga tgcgatagag ttcagttgat
     2221 ggcgtcactg caatcagtgg cgcgagcgtc tcgggaatat cgctggtacg gtttttctcg
     2281 gtaaaccccc aagaaggctc tcgcccttgc tcaattgcgg cccgctgaaa gctcgccgac
     2341 agctccggac tgtaattaaa tcgcggctgg gtcacgaacc accccttacg atccttacgg
     2401 aagattttgg attctgcctc gaggttcaac aacgcctggc gcagcgtcat acgtttaatg
     2461 cccagcagtt ctcccagctc tctttctgaa gggagttttc cacccggcgt ggtgatgcca
     2521 tctttcaacc accgatccag tttctctttt gcattttcta cggtggattg attctccgcc
     2581 attgtttaat ccttttggtt taaaccagat gaagcatctt acgcttacgc caaaaaagaa
     2641 agcaattaac ggtgtatgac tttgcagata attgtgacaa agctctctat tgacgtaatc
     2701 acagacttat aagggagata ctcgcattgg tgtgcgctca cgacaggtaa aaaaaaacct
     2761 gccatcgctg gcaggttttt tatgactaaa aaacgaaatt aatcgtcttt cggtgcgcgg
     2821 ttggcgcggc gacgatcgtt ttccgtcagg tgacgtttac gaatacggat agaggtcgga
     2881 gtcacttcta ccagttcgtc atcatcgatg aactccagag cttgttccag agtcatgcgg
     2941 ataggcggaa ccagaacaac agcttcgtca gtaccggaag cacgcatgtt ggtcagtttc
     3001 ttaccggtca ggcagtttac agtcaggtcg ttagagcggc tatgaatacc gataatctga
     3061 ccttcgtaaa cttctgcacc gtgaccgagg aacagcttac cgcgatcctg cagaccgaac
     3121 agcgcgaacg cgacagcttt accctgaccg ttagagatca gtacaccgtt ctgacgctga
     3181 cccacttcgc ccggacgtac gtcgtcgtag tggctgaagg tggagtacag cagaccagta
     3241 ccggaagtca tggtcatgaa ctcagaacgg aagccaatca gaccacggct tgggatcacg
     3301 tagtcgagac gtacgcggcc tttaccgtct ggattcatgt ttttcaggtc gcctttacgt
     3361 tcgcccagcg cctgcatcac agaaccctga tgctgttctt caacgtccag cgtcacgttt
     3421 tcatacggct cttgtttacg accgtctatt tcacggaaga ttactttcgg acgggatacc
     3481 gccagttcga aaccttcacg acgcatgttt tcgataagaa cagagaggtg cagttcacca
     3541 cgaccagaaa cgcggaacgc atcggcgtct tcggtttctt ctacgcgcag cgcaacgttg
     3601 tgtaccagtt ctttgttcag acgatcgagg atctgacgag acgttacgaa cttaccttct
     3661 ttaccgcaga acggcgaggt gttaacgcag aagaacatag aaacggtcgg ctcatcaacg
     3721 gagagtgccg gcagcgcttc aacgttttgc gtgtcgcaaa cggtgtcaga aatgttcagt
     3781 tcgcccaggc ccgtgatcgc cacgatatcg ccagcttccg ccagatcggt ttcgatacgt
     3841 tccagaccga ggtggcccag cactttaccg actttcgcgt tacgggtttt gccttcgcta
     3901 tcgatgatag taacctgctg gttcggcttc actttaccac gcttgatgcg gccaatgccg
     3961 ataacgccaa catagctgtt gtaatcgagc tgagaaatct gcatctggaa cggaccgtca
     4021 aggtcaacgt ccggcgcagg aacgtggtca acaatcgcct gatacagcgg ggtcatgtct
     4081 tccgccatat cttcgtggtc cagacccgcg ataccgttca gcgcagaagc gtaaacgatc
     4141 gggaagtcca gctgctcgtc ggtcgcgtcg aggttaacga acagatcgaa tacctgatcc
     4201 acaacccaat caggacgcgc gccagggcgg tcaactttgt tgataacaac aatcggcttc
     4261 aggccgtaag caaacgcttt tttggttacg aagcgcgttt gcggcatcgg gccgtcaaat
     4321 gcgtcaacca ccagcagcac tgagtctacc atggacatta cacgttcaac ttcaccaccg
     4381 aagtcggcgt gccccggggt atcaacgatg ttgatacggt aatcattcca tttgatagcg
     4441 gtgtttttcg cgaggatggt aatcccacgc tctttctcca aatcgttgga gtccatcacg
     4501 cgctcttggg tttcggcacg agagtcgaac gtaccggatt gttggagcag cttgtctacc
     4561 agggtggttt taccatggtc tacgtgcgcg atgatggcga tattacgcaa tttttcgatc
     4621 acaactttgc ctcaggcatt agaaatagcg cgttattgta cacggattaa tcgcactaca
     4681 aaacaggatc acaaacatcc tccgcaaaca agtattgcag agtccctttg tgatcgcttt
     4741 cacggagcat aaaaagggtt atccaaaggt cattgcacca atatggtgct taatgtttcc
     4801 attgaagcac tatattggtg caacattcac atcatggtgc agcccttttg cacgatgtta
     4861 agcatgataa cgccttttag gggcaattta aaagttggca cagatttcgc tttatctttt
     4921 ttacggcgac acggccaaaa taattgcaga tttcgttacc acgacgacaa tgaccaatcc
     4981 aggagagtta aagtatgtcc gctgaacacg tactgacgat gctgaacgag cacgaagtga
     5041 agtttgttga tttgcgcttc accgatacca aaggtaaaga acaacacgtc actatccctg
     5101 ctcatcaggt gaatgctgaa ttcttcgaag aaggcaaaat gtttgacggc tcctcgattg
     5161 gcggctggaa aggcattaac gaatccgaca tggtgctgat gccagacgca tccactgcag
     5221 tgattgaccc gttcttcgcc gactccaccc tgattatccg ttgcgacatc cttgaacctg
     5281 gcaccctgca aggctatgac cgtgacccgc gctctattgc gaagcgcgcc gaagattacc
     5341 tgcgttccac tggcattgcc gacaccgtac tgttcgggcc agaacctgaa ttcttcctgt
     5401 tcgatgacat ccgtttcgga tcatctatct ccggttccca cgttgctatc gacgatatcg
     5461 aaggcgcatg gaactcctcc acccaatacg aaggtggtaa caaaggtcac cgtccggcag
     5521 tgaaaggcgg ttacttcccg gttccgccgg tagactctgc tcaggatatt cgttctgaaa
     5581 tgtgtctggt gatggaacag atgggtctgg tggttgaagc ccatcaccac gaagtagcga
     5641 ctgctggtca gaacgaagtg gctacccgct tcaataccat gaccaaaaaa gctgacgaaa
     5701 ttcagatcta caaatatgtc gtgcataacg tagcgcaccg cttcggtaaa accgcgacct
     5761 ttatgccaaa accgatgttc ggtgataacg gctccggtat gcactgccac atgtctctgt
     5821 ctaaaaacgg cgttaacctg ttcgcaggcg acaaatacgc aggtctgtct gagcaggcgc
     5881 tgtactacat tggcggcgta atcaaacacg ctaaagcgat taacgccctg gcaaacccga
     5941 ccaccaactc ttataagcgt ctggtcccgg gctacgaagc accggtaatg ctggcttact
     6001 ctgcgcgtaa ccgttctgcg tctatccgta ttccggtggt ttcttctccg aaagcacgtc
     6061 gtatcgaagt acgtttccca gatccggcag ctaacccgta cctgtgcttt gctgccctgc
     6121 tgatggccgg tcttgatggt atcaagaaca agatccatcc gggcgaagcc atggacaaaa
     6181 acctgtatga cctgccgcca gaagaagcga aagagatccc acaggttgca ggctctctgg
     6241 aagaagcact gaacgaactg gatctggacc gcgagttcct gaaagccggt ggcgtgttca
     6301 cggatgaagc gatcgatgcg tacatcgctc tgcgtcgcga agaagatgac cgcgtgcgta
     6361 tgactccgca tccggtagag tttgagctgt actacagcgt ctaatagttg aagttgtact
     6421 acccggcgca caacgccggg atttagttgc cgtggaaact ttcagcccat ctctgcatgg
     6481 gcttttttct ccgtcaattc tctgatgctt cgcgcttttt atccgtaaaa agctataatg
     6541 cactaaaatg gtgcaacctg ttcaggagac tgctttatgg caacaggcac gcagcccgat
     6601 gctgggcaga tcctcaactc gctgattaac agtattttgt taattgatga caatctggcg
     6661 atccattacg ccaaccccgc cgcgcaacaa ctgctcgccc aaagctcccg caaattgttt
     6721 ggtacgccgt taccggaact gttgagctac ttctcattaa atatcgagct gatgcaagaa
     6781 agtctggagg cggggcaagg ctttaccgat aacgaagtga cgctggtcat cgatggtcgc
     6841 tcgcatatcc tttctgtgac ggcccagcgt atgccggacg gcatgatcct gctggagatg
     6901 gcaccgatgg ataaccagcg ccgcttaagc caggaacaac tacaacatgc tcaacaagta
     6961 gctgcccgtg atttagtgcg cggcctggca catgagatta aaaatccgct tggcggttta
     7021 cgtggcgcgg cgcagttact cagcaaagcg ttacctgacc catcactact cgaatatacc
     7081 aaagtgatta tcgaacaggc ggaccggctg cgaaatttgg tcgaccgtct gttggggccg
     7141 cagctgcccg gtacgcgcgt taccgaaagt attcacaaag tggctgaacg cgtggtaacg
     7201 ctggtgtcga tggaactgcc ggacaacgtg cggttgattc gtgattacga tcccagcctg
     7261 ccggaactgg cgcacgaccc ggatcaaatt gaacaggtct tgctgaatat tgtgcgcaat
     7321 gcgctacagg cgctggggcc ggaaggcggt gaaatcattc tgcgtacccg caccgcgttt
     7381 caactgacct tacacggcga gcgctaccgg ctggcggcgc ggattgatgt ggaagataac
     7441 gggccgggca ttccgcctca tttgcaggat acgctgtttt acccgatggt cagcggccgc
     7501 gaaggtggca ccgggcttgg cttatccatc gcccgtaatt tgattgatca gcattcaggc
     7561 aaaattgaat ttaccagttg gccaggtcat accgagttct cggtttacct gcctatcagg
     7621 aaataaaggt gacgtttatg caacgaggga tagtctgggt agtcgatgac gatagttcca
     7681 tccgttgggt gcttgaacgt gcgctcgctg gagcgggttt aacctgtacg acatttgaga
     7741 acggcgcgga agtactggag gcgctggcga gcaaaacgcc ggatgtgctg ctttcagata
     7801 tccgtatgcc gggaatggac gggctggcgc tgctcaagca gattaaacag cgccatccaa
     7861 tgcttccggt catcattatg accgcacatt ccgatctgga tgctgccgtc agcgcctatc
     7921 aacaaggggc gtttgattat ctgcccaaac cgtttgatat cgacgaagca gtggcgctgg
     7981 ttgagcgcgc tatcagtcat taccaggaac agcagcagcc gcgtaatatt cagcttaacg
     8041 gcccaacgac cgatatcatc ggcgaagcgc aagccatgca ggacgtgttc cgtattatcg
     8101 gtcggctttc gcgttcttct attagcgtgc tgattaacgg cgaatccggc accggtaaag
     8161 aactggtcgc tcatgccctg catcgccaca gtccgcgcac caaagcgccg tttatcgcgc
     8221 tgaatatggc agctatccca aaagatttga tcgaatcaga actgtttggc cacgagaaag
     8281 gcgcgtttac tggcgcgaat accattcgtc aggggcgttt tgaacaggcc gatggcggca
     8341 cgttatttct cgacgaaatt ggtgatatgc cgctggatgt gcagacgcgt ttgctgcgcg
     8401 tgctggcaga cggtcagttt tatcgcgttg gcggctatgc gccggtgaaa gtggatgtgc
     8461 ggattatcgc tgccactcac cagaatcttg aacagcgggt gcaggaaggt aagttccgtg
     8521 aggatctgtt ccaccgcctg aacgttatcc gcgttcatct gccgccgctg cgcgaacgtc
     8581 gggaagatat tccccgtctg gcgcgccatt ttttacaggt tgccgcgcgc gaactgggcg
     8641 tagaagcgaa gttgctgcat ccggaaaccg aagctgctct gacgcgtctg gcgtggccag
     8701 gcaacgtgcg ccagctggaa aacacctgcc gctggctaac ggtgatggcc gccgggcagg
     8761 aagtgttgat tcaggatttg cccggcgaac tgtttgaatc aacggttgcg gagagtactt
     8821 cgcaaatgca accggacagt tgggcgacac ttttagcgca gtgggcagac agagcgctgc
     8881 gttccggtca tcaaaatctg ctttccgaag cgcagccaga gctggagcgg acgttactga
     8941 cgaccgcgtt gcgacatacg caggggcata aacaggaagc ggcgcggcta ctcggctggg
     9001 gccgcaacac tctgacgcgt aagttaaaag agctggggat ggagtgattc acggcttgtg
     9061 tgtaaagatt gattattgag cgcaaattgc tggtattttg cgctttactg ttccgataag
     9121 ttcagtatga tcttgcccgg aaaacgggga gagtcattat gctggaatca ataattaatc
     9181 tggtatcgag tggcgcagtt gacagccaca caccgcaaac tgccgttgct gccgtgctgt
     9241 gtgccgcgat gattgggctg tttagctgaa gctcgttgtt tatgccggat gcggagaaag
     9301 tcgtaggcct gataagacgc gaccagcgtc gcatcaggca tcatgctcag atgcctgatg
     9361 cggcgtaaac gccttatccg gcctacaaaa gcaataattt caacaggttt tgttgcccag
     9421 cgcatcaggc aatcttgttc tcagcacatt gatggcgctt tcgttttcta ctttgtaaac
     9481 gaagcgccat tcactcttta aatcacccgt gagaactgct gcatccgcgc tttctggcgc
     9541 agataggtat caaagcacat gcaaatgttg cggatcagca agcgaccttt cgccgtcacc
     9601 tgtattccct tctcatccac atccaccagc ccatcttttg ctaacggggc gagcagcttg
     9661 agatcttccg caaagtaatc agcgaagtgc aaatcccact gtttctcaag aggggcgtaa
     9721 tccagacgga agttgcagat gagcgactta atcacatcgc ggcgaataca gtcatcacgc
     9781 gttagcgcaa taccacgcca cagcgcattg ccttgttcat acacttgctg atagtactgc
     9841 ttcaactctt tctggttctg cgcgtagcag tcgccaatca tgctgatggc ggaaacgccc
     9901 atccccagca gatcggtatc gccctgagtg gtgtagccct ggaagttacg atgcagcacg
     9961 ccttcacgct gggccaccgc cagctcgtca tcaggacggg caaagtgatc cataccgata
    10021 aactgatagc ccgattgcgt caggaaggcg atggtttcct gcaggatatc gagtttttgc
    10081 tgcggactcg gcaggtcagc atctttgatt ttgcgctgag cagcaaaaat ggtcggcaga
    10141 tgcgcgtagt taaaaacgct cagacgatcg gggttcagct ccgccacacg tttcagggta
    10201 aaggcgaaac tctccggcgt ctgtttcggc aggccgtaaa tcaggtcgat gttggtggag
    10261 gtaaaaccaa tctcacgcgc atggttaagc agtgcaaaga tgaactcttc atcctgctcg
    10321 cggttaacca gacgttgcac ttctttgttg aagtcctgca cgcccatgct caggcgatta
    10381 aagccttcgg cgcgtaaatg atcgagtaca tccagttcga tttcccgcgg atcgacttcg
    10441 atcgaaatct ccgcatcggt attgaaatgg aagttttcgc gcagcagctt catcagacgg
    10501 ctgatttgcg ctttattcag atacgtcggc gttccgccgc cccagtgcaa ttggctgacg
    10561 tgacgcccgg cgaacagcgg tgcacgatgg acgatttctt gctccagcgc gtccagatac
    10621 tgatcggcct tgtgctgctg gcgagtaaca atcttattgc aaccgcagaa gtaacaaagc
    10681 ttatggcaga acgggatatg tacgtagaga gataatggac gctcaggata gcgcgccacg
    10741 gcttgtaaaa acgcctgttc gccgaagtct tctgaaaact ccagcgcggt cgggtacgag
    10801 gtgtatcgtg gcccggaata gttatatttc tggatcaggg ccagatccca gtcgatttgc
    10861 tgtacagaca tgctcactcc ttccgatggc gtctctggcg gctacggcgt accggcggat
    10921 gcccgtttcg ggtcatcagc cggttgcgca gccacttttg tcgccgcgac aaccgctgta
    10981 gtttaacgaa taaccacacc agataacata taaccggaag ggttatcagc agaacgggaa
    11041 ggcccacggc ggcaatccgt tagttgcccc gcagcagacg catcatgtct tcttgcttct
    11101 cgtcttcttc ctcttcttcg tcatcgtcat aagagaggcc gagtttctgc atcaactcat
    11161 caatacgatc cagtttagca tccacccagg attgctcttc ggcgctcagg gtttcgcctg
    11221 cttccagacg ttccagcagc gcatccagac actcatccgt ttccagtaat tccaactccg
    11281 cctgcggtga aagcataggt ttctcactct tcggtttgtg ctgtttggtg actttttcag
    11341 tcactcccaa tggaataggg gttttactgc caatacgtgg atcttttggt gcgttctggc
    11401 ctttgctgcc tgacgtggtg tttccgcccg ctgcgcggct gcccggcgca tgaccacgac
    11461 gttttttctg acgcttacgg tcacgagctt cctgatccag ctcctcgcgt gtttttcgac
    11521 gcgcttttgc atgacctttg ctgcgtgagt ttgaagatga tggtttcata atggttcgtc
    11581 ttttagccgt tttattcagt atagatttgc ggcggaatct aacagaaagc aagcaaagaa
    11641 aaaaggcgac agattactct gtcgcctttt ttcctgactc ataaccctta acgggtctta
    11701 caatccttgc aggccgccaa cacaccctgt ttttccatta gccggaacaa tccgtgttga
    11761 ttccatttcc gtttaattac gtctccggac gtttgtcttc ctgacaatcc tctgtcttcg
    11821 cctcctggcg ctcctgaccc tcatcctgag tcattgttcc tgttagcgtc ctcgctctat
    11881 gggtgctact ttacgccttt gctttaaaca aacaatcaac cgccatcagt atttgcacat
    11941 tttcagagtt ttatataaaa gcaattcact gatttatata gcttatttga catgcatcgc
    12001 agaattttct ctgctatttc gcataaagac tatcggcaat gtctcacaaa gcgtatggct
    12061 tttacttaca acgctcacgc cgaaaaatcc gccttttatt atggttcagg tataatcgcc
    12121 acaaagcttt gactgacgga gacgaccgct ttgactaatt tgaattatca acagacgcat
    12181 tttgtgatga gtgcgcctga tattcgccac ctaccttccg ataccggaat tgaagtggct
    12241 tttgcaggcc gttccaacgc aggtaaatcc agcgcgctga acacgctgac taaccagaaa
    12301 agcctggctc gtacctcaaa aaccccaggg cgcacccagc ttatcaacct gtttgaagtg
    12361 gctgacggca agcgtctggt tgacttgcct gggtacggtt atgcggaagt cccggaagag
    12421 atgaagcgca aatggcagcg tgcgctcggc gaatacctcg aaaaacgtca gagcctgcaa
    12481 ggtctggtgg tgctaatgga tattcgccat ccgctgaaag atttggatca gcagatgatt
    12541 gagtgggcgg tagacagcaa tatcgccgtt ctggtgctgc tgaccaaagc ggacaaactg
    12601 gcaagcggcg cacgtaaagc gctattgaat atggtgcgtg aagctgtact ggcgtttaac
    12661 ggtgatgtgc aggttgaaac gttttcttcg ttgaagaaac aaggcgtaga caagctgagg
    12721 cagaaactgg atacctggtt tagcgagatg cagcctgtag aagaaacgca ggacggcgaa
    12781 taattttctt gccttaatgc ttgtgccgga tgtggcgtat ccggcccgta aattcaatag
    12841 attacaattc taccgttggc ctgataaaca tagcgcatca ggcattacgg atcttttctt
    12901 tcgcccaata aaaaacgccc cagtcattac tgactggggc ggctaaaata ttcagccaaa
    12961 tccgattacg tgaagtaaaa ggtctgaaag atagaacatc ttacctctgt accctacgcg
    13021 actaacttta ctcttttttg ttcagttcag aaagcacttt ttgtaatttt ttttcagttg
    13081 ttgcatagga atttctaata gccatcacaa aacgtgacag cttatgttgc ttacttacga
    13141 aaaaagggca tgttcaggcg aatcttagtg cgcctgatcc cagttttcgc cactccccac
    13201 ttccaccagc aacggcacat ccagacgggt acagttttcc atcagttgat gaatctgctt
    13261 cgcgacggca tcaacatcat ctttatgaac ttcaaatacc agttcatcgt gtacctgcat
    13321 gatcatacgt acacgcggtt gctcagcctg taaccacgca tcaacggcaa tcatcgcccg
    13381 tttgataatg tcggcggcgg ttccctgcat tggcgcgtta atggctgcac gttcagccgc
    13441 tgcacgacga gcaccattgc tggatttgat atccggcaga tacagacggc gtccgtccag
    13501 cgtttcaacg tagcccagct ctttcgcctg agcacgggtg cgttccatat actccagcac
    13561 gccagggtag cgttcgaagt aaaggtccat gtacttctgc gcttctttac gtggaatgtt
    13621 caattgccgc gccagaccga aagcactcat gccataaatc agaccaaagt tgatcgcttt
    13681 cgcgctacgg cgttgctcgc tggtgacggt ttccagtggc aaaccaaaca cttctgccgc
    13741 cgttgcccgg tggatatctt ttccttccgc gaatgcggtc agcaagcctt tatcacgcga
    13801 gagatgcgcc ataatgcgca gttcaatctg cgagtagtcc gctgagacaa tcacataatc
    13861 ctctggcgca ataaacgcct ggcggatacg acgaccttct tcgttacgca ccggaatgtt
    13921 ttgcaggtta ggatcggttg acgataaacg tcccgttgca gttactgcct ggtgataaga
    13981 ggtatgcaca cgcccggttt tcgggttgat catcagcggc agcttgtcgg tgtaagtcga
    14041 cttcagcttc gccagaccac gatactccag aatcactttt ggcaacggat agtccagcgc
    14101 cagttcttcc agtacctctt ccgacgttga cggcgcgcca cccggcgttt tcttcagcgg
    14161 tttaataccc tgtttttcaa acagaatggt ttgcaactgc ttggtggaag aaaggttaaa
    14221 ttcttcacct gcaatttcat gcgctttctt ttccagctca gccagacgca aggtgagctc
    14281 ttcagaatga ttgtgcagca ctttcggatc gatcttcaca ccgttacgtt caatgcgtga
    14341 aagcaccggc accagcggca tttcgatatt ctcgaagacg ttcaacggcc ctttgtgttt
    14401 ctgcagatcc ggccacattt tcagatgcaa ctgcaaggtg acatctgcat cttcggcggc
    14461 gtaacgtccg gcctcttcga gggcaatctg gttaaaggtc agttgatttt tgcctttacc
    14521 cgcaatctct tcaaaagtga tggttttgtg cttcaaccaa cgttccgcga ggctgtccat
    14581 atcgtgacgc ccggcaacgc tattgagaat gtaggactcc agcatggtat caaacgcaat
    14641 cccacgcagt tcaatgccgt aattcgccag aataccgcga tcgtatttca ggttttgccc
    14701 gaccttcagc gccttttcat cttccagcag cggttttagc aactcgagtg cacgctcgtg
    14761 agagatttga tcgggcgcat caagataatc atgagcaacc ggaatatatg ccgctacgcc
    14821 tggctcgata gcaaaagaaa gcccgaccag gttagcagag atgttatcaa ggctgtcggt
    14881 ttcggtatca aatgcaaata ccggcgcttt ttccagcttc gcaatccacg ctttcagtgt
    14941 ttcttcatca aggatagtga cgtagttgtc ataagaaatc accgttgccg tcacttctgg
    15001 cgcttcgtct gcaacactgg tttcctgagg cttcgcggct gtttttgccc ctttggcctg
    15061 taaccatttg cccacttcga catcagcagt ccagcgtttg aactcatact ttttgaacag
    15121 ccccaacaac tcttccgctg ccggttgctg cacttccagt tgttcacagg tcagctccag
    15181 ttcaacatcg gttttaatcg tcgccaactg gtatgagaga taagcaactt ctttgttttg
    15241 ctcgagcttc gctgccattg ttttcgcgcc acggaagctc aacccagcaa ttttttctgg
    15301 ctcggcatac agcgtatcca gcccgccaag accttgcagc aatgcctgcg cggttttttc
    15361 accgacgccc ggtacgccag gaatgttatc agaggagtca cccatcagcg ccaggaagtc
    15421 gatgatcaat tctggcggca cgccgtactt attcaccacc tcttccggtc cgaggatggt
    15481 attcgtcatg gtattgataa gcgtaatatt tggcgtcacc agctgcgcca tatctttatc
    15541 gccagtgcta atcagcaccg gacgcccggc tttttcggct tcgcgcgcca gagtaccgat
    15601 aacgtcgtcc gcttctacgc cagaaaccgc cagcagcggc agtcccatcg ctttaaccat
    15661 cgcgtgcaga ggttcgattt gtgctcgcag atcgtccggc attggcgggc gatgtgattt
    15721 gtaatgttca aacagttcat cacgaaaggt ttttcccttg gcgtcaaaga ccaccgctgc
    15781 atgcgtcggt ttatattgca tgatcagact gcgcagcatg ttgaggacac catacatcgc
    15841 accggtcggc tcgcctgcgc tgttagtcag cgggggaaac gcgtgatatg cgcgataaag
    15901 ataagatgaa ccatctacaa ggataagtgg attttggggg atctgaacca taatgtccgt
    15961 gcctgtttat cagattatgg gtaaaggatg ccacagacag gatgaaaaca tgagtttttc
    16021 accgcttttc gtgaaaatct tttgtagatc tgctggatcg ctcgcaaatc gtcatgtgga
    16081 taactttgtg cataaattat ttacatcgct tttaatttat tatcatgctt tttacagcat
    16141 gatgtttgtc tttgtttttc atgcagttaa aaataaatcc atccctaata attgagataa
    16201 tgggattttt tctaatgtgg atagatatga attatttttc tccttaagga tcatccgtta
    16261 tttgggtcat tttctgctaa aatctgcgcc ttgcgcttct ttagtgcact gtctatggct
    16321 aaccttttga ataaattcat tatgacgaga atactcgctg cgataaccct tttgctgagt
    16381 atcgtattga ccattttggt cactattttt tgttccgtcc cgatcatcat tgccgggatt
    16441 gtaaaactat tgctacctgt gccagtcatt tggcgaaagg tttcgcgttt ttgtgatttt
    16501 atgatgtatt gctggtgtga aggtctggcg gtattactgc atctgaaccc acacctgcaa
    16561 tgggaagttc acgggctgga agggttaagt aagaagaact ggtatctgct tatttgtaac
    16621 caccgtagtt gggcagatat tgtcgtactg tgcgtgctgt ttcgtaagca cattccgatg
    16681 aataaatatt tcctcaagca acagctggcc tgggtgccat ttcttggcct ggcgtgctgg
    16741 gcgctggata tgccgtttat gaagcgttat tcccgcgctt atttattacg tcatccggag
    16801 cgacgtggtc aagatgttga aaccactcgg cgttcttgtg aaaagtttcg cctgcatccc
    16861 accaccattg tcaatttcgt tgaaggctcc cgtttcacgc aagaaaaaca tcagcaaacc
    16921 cactccactt ttcaaaactt gttgccacca aaggcggcag gcattgcgat ggcgctaaat
    16981 gtactgggta aacaattcga taaactgctt aatgttacgc tgtgttatcc ggacaataac
    17041 cgtcagccgt tcttcgatat gttaagcggt aaactgacgc ggattgtcgt acatgtggat
    17101 ttacagccta ttgccgatga gttacatggc gactacatca atgacaaaag ctttaagcgc
    17161 cattttcagc aatggctgaa ctcattgtgg caggaaaaag accggcttct gacgtctctc
    17221 atgtcatcac agcgtcagaa taagtaagtt ttactcgcct ctgaatgagc agaggcgagt
    17281 gagtatttta atgaacaaaa cgtccggcac gagacataaa ttcttcttcg ctcatctcct
    17341 gtccgttaaa aacaacttta cccggtgtat aacgcagctg cagcgaggcg gtattgttgt
    17401 caatcgtgat taactgaaac atctgaccta atgtcatcat cccgctgatt tgtttatcag
    17461 cctgcttttg cgctttttcc gcatccattc cttcagataa attaagctgt tttgcggttt
    17521 ctgtgacgac attaagcggt aacttcacat caaaattgag cgatttgata tctttgtttg
    17581 tggatgatga agatttggct gggtcggcaa tactgatatc cagattggca tttagttcgc
    17641 cgagtgcgtt cttccatctt accggttgtt taatggtcgg ctcacttttt tgtaataacg
    17701 gtgaatactc tttgaacaat gcagcattca cttcttgcag agcaacttca tcgtttgcta
    17761 actcagggtg tgcagcaagc tgcttctgca tcgcaatgtt atactggatg ataaattggc
    17821 gcactgctga gggatcaata gattcagcaa tcaatgacca ctcaccacta ccgagttgct
    17881 gattcccttt tgttaactta tcaagttcgt aggtcagatt gacattgata taatctttgt
    17941 ctttaacgcg atccagcctg gtttttgcaa cgaaagcatc gatttgggca acgtcttccc
    18001 cgtgattgat aatgttaatc tgattaaatt tcgactcgct ttcaccaacc ggaaatttct
    18061 tttcttctaa tcgcgccaga gaatcaatgg taaaactttt tgccgttact ttgttcattg
    18121 gtgataattg cagaacaata tcttcgactt tgccttcaat atccaggtta gacaatgaac
    18181 taccatcacc attaaaagta aactggccct caccaaaaga aaattttcca tactctgcag
    18241 gggcaacatc taatgtcgta gagaatgagc caccaaacgc gaatcgcaat gtcgcttcca
    18301 cgggcgattt atttttcgcg gcgataaata gcggttgtgt cagttcatta ttcactaagt
    18361 tcacttttgc cgctgccagt gctgggatga cattaccatg cattaacatt gtgatgggca
    18421 acggaccgtg ctcaacatcc acatcaaata caactttctg gcctggcttg atattgagat
    18481 cgggtgcacc gttatcgaat gtcatttgca tttgaaaacg ggaattaaaa aagcctttgt
    18541 caaagttttt ataacttaac gtcatatcgt aggcatgttc acctttcttt ttcgcatcgt
    18601 taaaatcttt aataaacttt tcgataccag gctgaatttg cgtacctgtg taccatgtgc
    18661 caccacccca gattacggct aacgcaacaa taacacctgt agctgactta cgtatcatat
    18721 aaatattccc tgataaaaca gcaaaacttt gaatatccac ttatgctgaa ataatgtcca
    18781 gcggcaggat gcattatcag aatgtattta tatttattaa ataataaaaa aagcccgtga
    18841 atattcacgg gctttatgta atttacattg aattattttt cctcggacag atatttcacc
    18901 gtatcagcat actgctgaac aaaaacatcc atattgctgg tatccatacc ctgcggattc
    18961 agctgatatt taccgttaac aaacatcgcc ggaacaccac gcaattgcac atcagctgcg
    19021 gctttttcct gctgagcgac cagagatttc accacgaagc tgttccacgc cgcgtcgtac
    19081 tcttcacctt taatacctgc attgataaat acatcgcgga tatcagatgc tgaacgaatg
    19141 gtctgggttt tctgtacgcc ttcaaacagc ggaacagtca ctttgtcttc cacgcccagc
    19201 gccatcgcca cagcccatgc ctgagtcaga tctttgccca ggtcaccacc catgaagttg
    19261 acgtggtatt tagtcatctt cacgccttcc ggcagttttt tcttcacatt atcagaaata
    19321 tgcagaactt cttcaaactg atagcagtgc gggcagaaga aagagaaaaa ctccagcact
    19381 tgcggcgcgc cagctaccgg tttttccagg gtagtgtact gtttaccatc ttcatactgc
    19441 gccgccgatg cgctaaacgc taaaactaaa ccagccagcg ccagccaaat ctttttcatg
    19501 atctactctc tccgattaat acataggtgt taattgcaaa gggggttctt gtagaacttt
    19561 tgcctgttct ataaaagtcg ccgtctgtcg tagccagtaa tcttccccgg ttaaccacgg
    19621 gaaatttttc gggaacgcgg gatcagccca atgccgcatt agccaggcaa gataataaac
    19681 caaacgcatg gcgcgtaaag gttcaatcag tccgatttca gcggtgtcga actcgctaaa
    19741 ttcttcataa gcttcaataa tagtttccag ttgcatccgc tgctgggctt tatcgccatt
    19801 gagcaacatc cacaaatcct gaatagctgg accattacgt gcatcatcca gatcaacaaa
    19861 cattggacca tcgcgccaga gaatattccc ggcgtggcag tctccatgta gccgcagaac
    19921 ggtgaaatct tcccgccagt gtgctgtaac ggcggcgatc agctcatctg tcgctttcag
    19981 aaatgccgct ttcaacccgg aaggtatcag tgtagcgtcc tcaaacagct tgcgaggctc
    20041 aatgagatat tcattcagac cgatggtcgg gcgatgaata aaaagctgtt tgcgccccgt
    20101 ctggtgcata cgccctaaat aacgcccaac cgcttccatc tgatcgatat tatcagcttc
    20161 gaactggcga ccaccgacgc ttggaaaaac agcgaaataa aatccctgat gattcaataa
    20221 agtctgaccg ttaaaggcca caggtgctgc gaccggaact tcatcattta ccagctgcaa
    20281 cgcaaattga tgttcttcga ggatttgatc ggctgtccaa cgttcagggc gataaaattt
    20341 gacgacaaaa cgtcgacgct cttcgtcctg aaattgatag acacggtttt catagctgtt
    20401 aagcggggta agaccggaat ccacccggat cccttgctta aacagagcgt ccatgatggt
    20461 atccgggtgt agtgtctgga aagtaaaagc gctgttgttc atcccatcat ccggaaaaca
    20521 ttacgaatga tgaaggatat catttcacga tgattttggt ggcgccgctt acaagctttt
    20581 actctttaat tacgccgcga gcgcgtaaca gcgccgtttt gaagtcttct tcataatctt
    20641 tctgcaaacc cgggatcacc gcatctttcg cggaatcacg cattttcagg tgatagatca
    20701 gaatgtcatc cgtcaaatcc gccagttcgc cgtcaaaacc tgactctttc gccagtttct
    20761 gcaaaaattg caacaagtta aagtctggct ctttttgcca ggctggctgg aggagttcaa
    20821 taacttcatt cagacgttta catttcatag taatgctcct tactcttgag acagacacgt
    20881 tagcagggtc aatcccacaa taaaagaggc gatatcggtg aatctaatga cgacgataac
    20941 aggcgttgtg ctggcaggcg gtaaagccag acgaatgggc ggcgtagata aaggattgct
    21001 ggacttaaat ggcaaaccgc tgtggcaaca tgtcgctgac gcgcttaaga cgcagctctc
    21061 tcacgtcgtg gttaatgcta atcgtcatca ggaaatctat caggcaggcg gtctgaaagt
    21121 gattgaagat tcactggcgg attacccagg ccctctggca ggaatgcttt cagtaatgca
    21181 gcaggaagct ggtgagtggt ttttgttttg tccgtgcgat acgccttaca ttccccatga
    21241 tttagccgcc cggcttaatc atcagcgcaa agatgcgcct gtcgtgtggg tccatgacgg
    21301 tgaacgcgat cacccgacta ttgctctggt aaaccgcgct attgagcctt tattactgga
    21361 atatctgcaa gcaggagaac gccgggtaat ggcatttatg cgtctggctg gcggtcatgc
    21421 ggttgatttc agcgatcata aagatgcatt tgttaacgtg aatacgccag aggagcttgc
    21481 ccgatggcag gaaaaacgat gataccgtta ctcgcctttg ccgcgtggag tggtaccgga
    21541 aaaactacgc ttttgaaaaa actgatcccg gcattatgcg ccagagggat ccgtccgggg
    21601 ctgattaagc atacgcacca tgatatggat gttgataagc caggcaaaga tagctatgag
    21661 ctgcgcaagg ctggcgcggt acaaaccatc gttgccagcc agcaacgatg ggccttgatg
    21721 acggaaacac cagacgaaga agagctggat ctacattttc tcgcaagtcg aatggatacc
    21781 tcaaagctgg atttgattct ggtcgaaggg tttaagcatg aagagatcgc aaagattgtg
    21841 ctgtttcgcg atggagccgg acatcgacct gaagaattag tgatagacag gcatgttatt
    21901 gctgtagcca gtgatgtgcc gcttaatctt gatgtcgcat tactggatat taatgatgtt
    21961 gaggggctgg ctgattttgt ggtggagtgg atgcaaaagc agaacggata agcaagagta
    22021 ggctggatca gacgc
//
LOCUS       DAJTQQ010000061        21822 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000061 DAJTQQ010000000
VERSION     DAJTQQ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21822
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.061"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..2102
                     /locus_tag="OO309_002572"
     CDS             <1..2102
                     /locus_tag="OO309_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCR5847677.1"
                     /translation="WDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGP
                     WRLRNNSTWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQ
                     LASDDNMLPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSS
                     SGDLLVEIKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQT
                     TLQWGGPWGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKG
                     QSYRFLYAKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWS
                     RYYNLFYTKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLS
                     LGVSWNYSKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEG
                     HITQNLGLTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNG
                     SQQQLNYALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTV
                     VPYATSYRENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSK
                     QGIPLRFGAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQL
                     TEQQINSAITRMDAICR"
     gene            2093..3076
                     /locus_tag="OO309_002573"
     CDS             2093..3076
                     /locus_tag="OO309_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5847678.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQHNIISPAGGTLPLSIPDDADSIRE"
     gene            3174..3714
                     /locus_tag="OO309_002574"
                     /pseudo
     CDS             3174..3714
                     /locus_tag="OO309_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            3722..4237
                     /locus_tag="OO309_002575"
     CDS             3722..4237
                     /locus_tag="OO309_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5847679.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4203..4940
                     /locus_tag="OO309_002576"
     CDS             4203..4940
                     /locus_tag="OO309_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5847680.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5051..6061
                     /gene="pyrD"
                     /locus_tag="OO309_002577"
     CDS             5051..6061
                     /gene="pyrD"
                     /locus_tag="OO309_002577"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR5847681.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6235..6777
                     /gene="zapC"
                     /locus_tag="OO309_002578"
     CDS             6235..6777
                     /gene="zapC"
                     /locus_tag="OO309_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR5847682.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(6774..7883)
                     /gene="ycbX"
                     /locus_tag="OO309_002579"
     CDS             complement(6774..7883)
                     /gene="ycbX"
                     /locus_tag="OO309_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR5847683.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8127..10235
                     /gene="rlmKL"
                     /locus_tag="OO309_002580"
     CDS             8127..10235
                     /gene="rlmKL"
                     /locus_tag="OO309_002580"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR5847684.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10247..12154
                     /locus_tag="OO309_002581"
     CDS             10247..12154
                     /locus_tag="OO309_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5847685.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12284..13537
                     /gene="pqiA"
                     /locus_tag="OO309_002582"
     CDS             12284..13537
                     /gene="pqiA"
                     /locus_tag="OO309_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR5847686.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            13542..15182
                     /gene="pqiB"
                     /locus_tag="OO309_002583"
     CDS             13542..15182
                     /gene="pqiB"
                     /locus_tag="OO309_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR5847687.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15179..15742
                     /gene="pqiC"
                     /locus_tag="OO309_002584"
     CDS             15179..15742
                     /gene="pqiC"
                     /locus_tag="OO309_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR5847688.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            15998..16165
                     /gene="rmf"
                     /locus_tag="OO309_002585"
     CDS             15998..16165
                     /gene="rmf"
                     /locus_tag="OO309_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR5847689.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16235..16753)
                     /gene="fabA"
                     /locus_tag="OO309_002586"
     CDS             complement(16235..16753)
                     /gene="fabA"
                     /locus_tag="OO309_002586"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR5847690.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(16822..18582)
                     /locus_tag="OO309_002587"
     CDS             complement(16822..18582)
                     /locus_tag="OO309_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR5847691.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            18767..19219
                     /gene="matP"
                     /locus_tag="OO309_002588"
     CDS             18767..19219
                     /gene="matP"
                     /locus_tag="OO309_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR5847692.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19295..20341)
                     /gene="ompA"
                     /locus_tag="OO309_002589"
     CDS             complement(19295..20341)
                     /gene="ompA"
                     /locus_tag="OO309_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR5847693.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(20697..21206)
                     /gene="sulA"
                     /locus_tag="OO309_002590"
     CDS             complement(20697..21206)
                     /gene="sulA"
                     /locus_tag="OO309_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR5847694.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            21425..>21822
                     /gene="sxy"
                     /locus_tag="OO309_002591"
     CDS             21425..>21822
                     /gene="sxy"
                     /locus_tag="OO309_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR5847695.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAIL"
BASE COUNT         5564 a         5134 c         5720 g         5404 t
ORIGIN      
        1 gttgggatga agggatcaac gcgctattac tgggatattc gtttagcggg gctaacagta
       61 ttcatagcag cgcagacagt gattctggcg acagctattt tctgaattta aacagtggcg
      121 ttaatttagg cccatggaga ttacgcaaca attcaacatg gagtcgcagt agtggccaaa
      181 ccgcagaatg gaagaatctc agcagctatt tgcagcgggc ggttattcca ctaaaaggcg
      241 aactgaccgt aggtgatgat tatactgcag gcgatttttt cgatagtgtc agctttcgtg
      301 gtgtgcagct ggcgtcagat gacaacatgc tgccagacag cctgaaaggg tttgcgcctg
      361 tggtgcgtgg tatcgccaaa agcaatgccc agataacgat taagcaaaat ggttacacca
      421 tttaccaaac ttatgtatcg cctggtgctt ttgaaattag tgatctctat tccacgtcgt
      481 cgagcggtga tttgttagtt gaaatcaaag aagcggacgg cagcgtcaat agctacagcg
      541 taccgttttc cagcgtgcca ttactccagc gtcaggggcg aatcaaatac gcggtgacac
      601 tggcgaaata cagaaccaat agtaatgaac agcaggagag caaatttgcc cagaccacgt
      661 tgcagtgggg cggaccgtgg ggaacgacat ggtatggtgg tggacaatat gctgaatatt
      721 accgtgccgc catgtttggt ctgggattta accttggcga tttcggagca atttcgttcg
      781 atgcgaccca ggcgaagagt acgctggcag accaaagcga acataaaggt cagtcatatc
      841 gttttctgta tgccaaaacg ctcaaccaat tgggcactaa ttttcaattg atgggctatc
      901 gctattcgac gtcgggtttc tacacccttt ccgacaccat gtataaacat atggatggct
      961 acgaatttaa tgacggtgat gatgaagata cgccgatgtg gtcgcgttat tacaatttgt
     1021 tttacaccaa acgtggcaaa ctgcaggtca atatctccca gcaattaggc gagtacggtt
     1081 cgttttattt aagtggtagc cagcaaactt actggcatac cgatcaacag gatcggctat
     1141 tacagtttgg ctacaacacg caaattaaag atctctcgct gggggtttcc tggaactaca
     1201 gtaagtcccg tggtcaacct gatgctgatc aggtgtttgc actaaatttt tccctgccgc
     1261 tcaatctgtt gctccccaga agtaatgata gctataccag gaaaaaaaat tacgcctgga
     1321 tgacctctaa caccagtatc gataacgaag ggcacattac acaaaacctg ggtttaacgg
     1381 agacactact cgatgacggt aacctgagct acagcgtgca acagggatat aacagcgagg
     1441 ggaaaacggc taatggtagc gccagcatgg actacaaagg ggcgtttgca gatgcccgag
     1501 tgggctacaa ctacagcgat aacggcagtc aacaacaact gaactacgct ctttcaggca
     1561 gtttagttgc ccattcacag ggcattaccc tggggcaatc gctgggggaa actaacgtgc
     1621 tgattgcagc accaggcgca gagaatactc gtgtggcgaa cagcaccggg ctgaaaactg
     1681 actggcgcgg atataccgtt gtcccctatg ccacttctta tcgggaaaat cgaatcgcac
     1741 ttgatgcggc atcgttaaaa cgtaacgtgg atcttgaaaa tgcagtagtc aacgtggttc
     1801 ccaccaaagg ggcgttggtt ctggcggagt tcaatgccca tgcgggagca agggtattaa
     1861 tgaaaacatc aaagcagggt ataccgctgc gttttggcgc gatagcgacg ctggacggca
     1921 tacagactaa tagcggcatt attgatgatg atggctcgct ctatatgtcc ggtttgcccg
     1981 ctcagggagc gataactgta cgctggggcg aagctcccga tcaaatttgt catatcagtt
     2041 accagcttac cgaacaacaa attaactctg cgattacgcg aatggatgcc atatgcagat
     2101 aatctttgga gaaaaatgcg tgttattact acgactattt tttgccgccg tcttaatgct
     2161 atggtgcgct caaactgctg cttatatcgg gcagtgtcat accactcagg ggaatccgta
     2221 tattggcgtc aattttggcg ttaaaactct ggatgaagaa gaaaatacgg ctggagtagt
     2281 taaagacaaa ttttatcagt ggaacgaatc gaatgattat tatgtttcct gtgattgcga
     2341 taaagacaat gtcagaagtg gccgatgggc attcgccgcg gattcaccgt tagtctattt
     2401 aggcgacaac tggtacaaaa ttaatgacta tcttgccgcc aaagttttat tgcaggttaa
     2461 aggcagttct cctactgcgg ttcctttcga aaacgtgggc acaggggcag atacacgatg
     2521 gcatatttgc gatcccggcg gtcaacgttt aggtggccag ggggctagcg gtaatagcgg
     2581 tagcttttcc ctgaaaatat tgcagccgtt cgttggttcg gtcgtcattc ctcctatggc
     2641 gctggcgcga ttatttgaat gctacaacat acccgcaggt gattcctgca cgactactgg
     2701 tacaccggtt ttagtgtatt acctgtctgg tacgatcaat tcacttggct catgttccgt
     2761 caatgccgga gaaacaatcg aggttgatct gggcgacgta tttgcggcta actttcgtgt
     2821 tgtagggcat aagcctcttg gggccagaac ggcagaactt gcaattccgg tcaggtgtaa
     2881 cacgggaaac gcagggttag ttaacgtcaa tctgagtctg acggcaacct cagaccccag
     2941 ctatccccag gcgattaaga cgtcacgtcc tggcgtgggc gtggtggtga ccgatagcca
     3001 gcacaacatt atttcccctg ctggtgggac actaccgctc tctattcctg atgatgcaga
     3061 cagtatccgc gaatgaatgt ctatccagtc agcacgacaa gcgtaccacc agaaaccggg
     3121 cgatttgaag ccacggcaac ggtgagaata aattttgatt aacgcgtgaa cgtatgaaga
     3181 aaaaaacgat atatcagtgc gttattttgt tctttagcct tcttaacatc catgtcggga
     3241 tggctgggcc tgaacaagtc agtatgcata tttatgggaa tgtggtcgat cagggctgtg
     3301 atgtcgccac caaaagtgca ttacaaaata ttcatattgg tgattttaat atcagtgatt
     3361 ttcaggccgc gaataccgta agcactgctg ctgatttgaa tattgatatc accggttgtg
     3421 ccgctggtat tactggcgcg gacgtccttt ttagcggcga ggctgacacc cttgcgccga
     3481 cactgctcaa actaactgac acaggcggaa gcggtggtat ggcaacgggg attgccgtgc
     3541 attcttgatg cgcaaagtca gcaagaaatc ccgctcaatc aggtccagcc tcttacgccc
     3601 ttaaaagccg gggataacac actcaaatat caacttcgtt ataagtccac aaaggcggga
     3661 gcaacgggcg gtaatgcgac ggcggttctc tattttgatc tggtttacca gtgaaggagt
     3721 gatgttgaaa cggataatct ggattctgtt cttactggga ttaacggggg gctgtgagct
     3781 atttgcccat gatggcacgg tcaacattag cggatcgttt cgccgtaata catgcgtgct
     3841 ggcacaggat agcaagcaaa tcaatgtgca gttaggcgat gtctcgctga cacgtttttt
     3901 tcatgggaat tatggcccgg agaaatcttt catcattaat ttgcaggatt gcggaacaga
     3961 tgtcagcacg gttgatgtca ccttttcagg aacccccgac ggagtgcaga gcgaaatgct
     4021 gtccattgaa agtggtacgg atgcagccag tgggctggca attgcgattc tggatgatgc
     4081 gaagatattg attccgctta accaggcgag taaagactac agcctacata gcggtaaggt
     4141 gccattaact ttttatgcgc aattgcgacc tgtcaatagc gatgtgcagt cgggtaaagt
     4201 gaatgccagc gcgacatttg tacttcatta tgactaatac gtggaacaga ttagcgctct
     4261 tgattttcgc cgttttatcg ctgctggtgg cgggtgaatt gcaggcagga gtggtggtcg
     4321 gcggaacgcg atttatcttt ccggcagaca gagaatcgat atctatttta ctgactaata
     4381 ccagtcagga atcttggctt attaacagca aaatcaatcg cccaacgcgt tgggccgggg
     4441 gggaagcgtc gacagtgcca gcactattac tggccgctcc gccacttatt ctcctgaagc
     4501 ccggtactac aggcacgttg cgcttgctga gaacggaaag cgacatcttg cctgtggatc
     4561 gcgaaacgct atttgagtta agcattgcca gcgtgccatc cggcaaagtt gaaaatcaga
     4621 gcgtaaaagt ggcgatgcgc tcggtattta aactgttctg gcgacccgaa ggtttgccgg
     4681 gcgacccgct ggaagcttat caacaattac gctggacacg gaattcgcag ggtgtacaac
     4741 tcactaaccc aacgccttac tacattaacc tgattcaggt gagcgtaaat ggtaaagcgt
     4801 taagtaatgc gggagtcgtg ccgcctaaaa gccagcgtca gacaaactgg tgtcaggtaa
     4861 tagcaccgtg tcatgtcgcc tggcggacga taaatgatta cggcgggttg agtgcaaaga
     4921 aggagcaaaa tctgccctga aacaggttct gaaaacgttt gcgttttttt tgccgcaggt
     4981 caattccctt ttggtccgaa ctcgcacata atacgccccc ggtttgcaca ccgggaatcc
     5041 aggagagttc atgtactacc ccttcgttcg taaagccctt ttccagctcg atccagagcg
     5101 cgctcatgag tttacttttc agcaattacg ccgcattacc ggaacaccgt ttgaagcact
     5161 ggtgcggcag aaagtgcctg cgaaacctgt taactgcatg ggcctgacgt ttaaaaatcc
     5221 gcttggtctg gcagccggtc ttgataaaga cggggagtgc attgatgcgt taggcgcgat
     5281 gggatttgga tcgatcgaga tcggtaccgt cacgccacgt ccacagcctg gtaatgacaa
     5341 gccgcgtctc tttcgtctgg tagatgccga aggtttgatc aaccgtatgg gctttaataa
     5401 tcttggcgtt gataacctcg tagagaacgt aaaaaaggcc cattatgacg gcgtcttggg
     5461 tattaatatc ggcaaaaata aagatacgcc agtggagcag ggcaaagatg actatctgat
     5521 ttgtatggaa aaaatctatg cctatgcggg atatatcgcc atcaatattt catcgccgaa
     5581 taccccagga ttacgcacgc tgcaatatgg tgaagcgctg gatgatctct taaccgcgat
     5641 taaaaataag caaaatgatt tgcaagcgat gcaccataaa tatgtgccga tcgcagtgaa
     5701 gatcgcgccg gatctttctg aagacgaatt gatccaggtt gccgatagtt tagttcgcca
     5761 taatattgat ggcgttattg caaccaatac cactctcgat cgttctctgg ttcagggaat
     5821 gaaaaattgc gatcaaaccg gtggcttaag tggtcgtccg cttcagttaa aaagcacaga
     5881 aattattcgc cgcttgtcac tagaattaaa cggtcgctta ccgatcatcg gtgttggcgg
     5941 catcgactcg gttatcgctg cgcgtgaaaa gattgctgcg ggtgcctcac tggtgcaaat
     6001 ttattctggt tttattttta aaggtccgcc gctgattaaa gaaatcgtta cccatatcta
     6061 attatttctt cgaattcctc tttataagat aatcagggct ttattttcag acctggttgt
     6121 tttatattca tcgttgttgc ttatttagac attttgtact tttattgttg aggttattaa
     6181 gcgaagcgac aatggattgt gttgctgcgg ttttataggg taggggagag gcagatgcga
     6241 attaaaccag acgataactg gcgttggtat tacgatgaag agcacgatcg aatgatgctc
     6301 gatttagcca atggtatgct atttcgctca cgttttgcgc gcaaaatgtt gactccagat
     6361 gccttttctc ccgcaggctt ttgtgttgat gatgccgcgc tctatttctc ttttgaagaa
     6421 aagtgccgcg attttaattt atctaaagac caaaaagccg aactggtgct gaatgcactg
     6481 gtagcaattc gttatctcaa accgcaaatg ccgaaaagct ggcattttgt ctcccatggt
     6541 gaaatgtggg tgcccatgcc gggcgatgcg gcctgcgttt ggttgagcga tactcacgag
     6601 caggtcaatt tgctggtcgt tgaatctggc gaaaatgccg cgctatgcct gctggcacaa
     6661 ccctgcgttg ttattgcggg tcgcgcgatg cagttgggcg acgccattaa aatcatgaac
     6721 gacaggctga aaccgcaggt gaatgttgac agcttcagcc tcgaacaggc agtctaacgc
     6781 gtcagcttaa gggcagtctt cggtacacag ctacagcaaa gaatggtgcc atcatcgccc
     6841 attgctgatt ttttcagcgg tgtgacttcg ccttctaaaa gctgaacacg gcaacttcca
     6901 caaatgcccg cgcggcaaga ataagggata cgaattccct gattttctaa ttgctccagc
     6961 aacacttgtt ggttatttcc acgaaatgcc tgtccctgcc agtcaatatc tacatttgcg
     7021 tccggttgtt gcgtgatgtt ggcggtatca tcagcggcag ctgcgccgta aatttttgcc
     7081 ggagccgttg ccagaatttc cacctcatcg ccaacgcgaa tcacgccgct attacgggca
     7141 attaaattct gaccaaaatc gacatcgccg ttatcctggg cagtgcggaa agattgcaat
     7201 gtttttaatg gttcgcctgc cggatgtttt tgcccttttt ctgggctgac ggtggtgaaa
     7261 atacagcggc tacaaggttt aaccacatca aacaccacat caccaatgcg aatcactttc
     7321 cagctatctt cttcccaggc tgacgcgccg gaaaccacca gattggggcg gaattgctcc
     7381 atttttacac tggccggaca acgttgttgg agatcacgta acgaggcttc gttagcaaga
     7441 aggtaaggat agccatcagc aaatgacaga ggtacagtgt tgtggcgttt cacgcgccgg
     7501 gtcatttgtg gccccaccca gcgtaattgc acttcgcggg agaaaaatcc acttagccat
     7561 ttgttgatcg cgtctggcgc aattcgcgcg gtaaaatgtg tgccccaaac ttcggttggt
     7621 gcgtcttgtg tggcgaaatc agcaaaacga acatatgcgc tactgccatc tggtgcggtg
     7681 agatgcaagc catcatgcac aggtgaagga gtaaaccgta ccatctgggg aaactggcga
     7741 gcggtaataa acgtaccgtc aggttccgtg atcataaaga tgcgatcgaa ggccagacca
     7801 ctgacatcag ccagagcatg tgtaagacca atgccgcgca tcgatttaac aggatgaata
     7861 aaaagccggg ttaatgtcgc cacggtgcgg tcctcaaatg aaaataagcc ctcaacttta
     7921 tgacatgacg cgcttattag ctataatgcg caacaatttt cttagcgcct gtcccgttag
     7981 gctattttat ttgtcagttt ggccctgggt tgtgctcgaa atcctcacgt acttgtgtac
     8041 gctccggttt ctccgcgctc tccatgtcca gactgactgc aacaattacg cctactgcgg
     8101 taggctctta gagtaaaagt gacgatatga attctctgtt tgccagtacg gcccgtgggc
     8161 tggaagagct gttaaaaact gaactggaaa atctgggggc cgttgaatgc caggtggttc
     8221 agggtggggt ccatttcaag ggcgacacac ggcttgttta ccagagcctg atgtggagcc
     8281 gcctggcctc gcgtattatg ttgccgctgg gcgagtgtaa ggtttacagc gatttagacc
     8341 tctatctcgg tgttcaggcg atcaactgga cagagatgtt taatcctggc gcgaccttcg
     8401 ctgtccactt cagtggtttg aatgacacca tccgcaacag tcagtacggc gcgatgaaag
     8461 tgaaagacgc gatcgtcgat gctttcacac ggaaaaatct gccgcgtcca aatgttgatc
     8521 gcgatgcgcc ggatatccgc gttaacgtct ggctgcataa agaaaccgcc agtatcgctc
     8581 ttgatctgag tggtgatggt ttacatctgc gtggctatcg cgatcgtgct ggtattgcgc
     8641 cgatcaaaga aaccctggca gccgctattg tgatgcgatc cggctggcag tcaggaacac
     8701 cgctgctcga tccgatgtgt ggttccggta cgttgctgat tgaagcggcg atgctggcga
     8761 ccgatcgcgc accaggcttg caccgtgggc gttggggctt tagcggctgg gcgcagcatg
     8821 atgaagctat ctggcaggaa gtgaaagcgg aagcgcaaac tcgcgcccgt aaaggcctag
     8881 ctgagtatag ctctcatttt tacggttcgg acagcgacgc acgggtgatt caacgtgcgc
     8941 gcactaacgc ccgtcttgcg gggattggtg aactgatcac ctttgaggtg aaagatgtcg
     9001 cgcaactgac caatccgctg ccgaaagggc cgtacggtac agtgttgagc aacccgccat
     9061 acggtgaacg tctggacagc gaaccggcgc tgattgcgct gcatagtctg ctgggccgaa
     9121 tcatgaaaaa ccagttcggt ggctggaatc tctctttgtt tagtgcctcg ccggatctgc
     9181 taagctgcct gcagctgcgt gcagacaaac agtacaaggc gaaaaacggc ccgctggact
     9241 gcgtacagaa aaattaccac gttgccgaaa gcacaccaga cagtaaaccg gcaatggtag
     9301 cggaagatta cgccaaccgc ctgcgtaaga acctcaaaaa attcgagaag tgggcacgcc
     9361 aggaagggat tgaatgttac cgcctgtatg acgccgatct gccggaatat aacgttgccg
     9421 ttgaccgtta tgccgactgg gtggtggtgc aggagtatgc gccgccaaaa actattgatg
     9481 cgcacaaagc gcgtcagcgt ctgttcgata ttatcgctgc aaccatttcg gtactgggga
     9541 ttgcgccaaa caaactggtg ctgaaaaccc gtgaacgcca taagggcaaa aaccagtacc
     9601 agaaactggg cgagaagggc gagtttcttg aagttaccga atataacgct cacttgtggg
     9661 tgaacctgac ggattatctc gatactggtc tgttcctcga tcaccgtatc gcccgccgta
     9721 tgctcggtca gatgagcaaa ggcaaagatt tcctcaacct gttttcttat accggcagcg
     9781 ccaccgtgca tgcgggatta ggcggtgcac gcagcaccac caccgtggat atgtcgcgta
     9841 cttatctgga atgggcagaa cgcaacctgc gtctgaatgg cctgactagg cgtgcgcatc
     9901 gcctgattca ggccgattgc ctggcgtggc tgcgtgaggc aaatgaacag ttcgatctga
     9961 tctttatcga tccgccaacc ttctctaact caaaacgaat ggaagatgcg tttgatgttc
    10021 agcgcgatca tctggcgctg atgaaagatt tgaaacgtct gctgcgtgca ggtgggacga
    10081 tcatgttctc gaacaacaaa cgtggcttcc gtatggatct cgacggcctg gcaaaactgg
    10141 gactgaaagc acaagaaatt acgcaaaaaa cgctctccca ggatttcgcc cgtaaccgcc
    10201 agatccacaa ctgctggctg attaccgcag cctgaaagga atagtaatgt cattaatcag
    10261 tatgcatggc gcatggctgt cgttcagcga cgcgccgctt ctcgataacg cagaactgca
    10321 tatcgaagat aacgaacgtg tttgtctggt gggccgcaac ggcgcaggaa aatcgacgtt
    10381 aatgaaaatc ctcaaccgtg aacaagggct ggatgacggt cgcattattt atgagcaaga
    10441 tttgattgta gctcgtctgc aacaggatcc gccgcgtaac gttgagggta gcgtttatga
    10501 tttcgttgcc gaaggcattg aagaacaagc ggaatatctg aaacgctatc acgatatttc
    10561 gcgcctggtg atgaacgacc cgagcgagaa aaatctcaac gaactggcga aggttcagga
    10621 acagctggat caccacaacc tgtggcagct ggaaaaccgc atcaacgaag tgctggcgca
    10681 actggggtta gatcctaacg ttgcgctgtc gtcgctttcc ggcggctggt tgcgtaaagc
    10741 ggcattagga cgcgcgctgg tgagcaatcc gcgcgtattg ctgctggacg aaccgactaa
    10801 ccatctggat atcgaaacca tcgactggct ggaggggttc cttaaaacct tcaatgggac
    10861 gattattttc atctcccacg accgttcgtt tatccgcaat atggcgacgc gcattgttga
    10921 tctcgatcgc ggcaagctgg tgacctatcc agggaattac gaccagtacc tgcaggaaaa
    10981 agaagaagcc ctgcgcgtgg aagaactaca aaatgccgag ttcgatctca aactggcgca
    11041 ggaagaagtg tggattcgcc aggggatcaa agcacgccgt acccgtaatg aaggccgcgt
    11101 acgcgccctg aaagcgatgc gtcgcgaacg tggtgaacgt cgcgaagtga tgggtaccgc
    11161 aaagatgcag gtggaagagg ccagccgctc cggtaagatc gttttcgaaa tggaagacgt
    11221 ttgctaccag gttaacggta agcaactggt gaaagatttt tctgcccagg ttctacgtgg
    11281 cgacaaaatt gccctgattg gtccgaatgg gtgcggcaaa accacgctgc tgaaactgat
    11341 gctcggtcag cttcaagcgg acagcgggcg tattcacgtt ggcaccaaac tggaagtggc
    11401 ttatttcgat cagcaccgcg cggaactgga tcccgataaa acggtgatgg ataaccttgc
    11461 cgaaggtaag caagaggtga tggttaatgg caagccacgc cacgtattgg gctatttgca
    11521 ggactttctg ttccatccga aacgggcgat gacgccagta cgtgcgtttt ctggcggtga
    11581 gcggaaccgc ttgctgctgg cgcgtttgtt cctcaaacca agcaacttat tgattcttga
    11641 cgaaccgacc aacgatcttg atgtcgaaac gctggaactg ctggaagaac tgatcgacag
    11701 ctatcagggc acggtattgc tggttagcca cgatcgtcag tttgtcgata acaccgttac
    11761 agaatgctgg atcttcgaag gcggcggtaa aattggtcgt tatgttggcg gttatcatga
    11821 tgcccgtggt cagcaagaac aatatgtggc actcaaacag cctgcggtga aaaaaagcga
    11881 agaagccgcc gcgccaaaag cagaaactgt aaaacgcagc agtagcaaac taagctataa
    11941 attgcagcgc gaactggagc agctaccgca attgctcgaa gatctggagg cgaagctgga
    12001 agccctacag acgcaagtgg cggatgcttc cttcttcagt cagccgcatg agcagacgca
    12061 aaaagtgctt gctgatatgg ctgctgcaga gcaggagctg gagcaagcct ttgaacgctg
    12121 ggagtatctt gaagcgttaa aaaatggtgg ctgatcgcaa aatagtcgat ttaaacagag
    12181 acagctgaca acggtaaaat tgcctgatga actgggttta tcaggcatga aaaccaacac
    12241 tttgttagtc atctgtaaaa tagcgcatca ttaaggagta ccaatgtgcg aacatcatca
    12301 tgccgcgaag cacatcctgt gctcgcagtg tgacatgctg gtggcgttac cgcgccttga
    12361 gcatggtcag aaagcggcat gtccccggtg tggcacaacg ttaaccgtgg cgtgggatgc
    12421 ccctcggcag cgtcccaccg cctatgcgtt ggctgcactg ttcatgctgt tgctgtccaa
    12481 cttgtttcct tttgtgaata tgaacgttgc aggagttacc agtgaaatta cattactgga
    12541 aattcccggc gtgctttttt ctgaggacta cgccagcctc ggcacctttt tcctgttgtt
    12601 tgtgcaactg gttcccgcgt tttgtctgat aaccattctg ttactggtga atcgcgcgga
    12661 attaccggtc cgtttaaaag agcaactggc acgggtgctt tttcaactca aaacctgggg
    12721 aatggcggag attttcctcg cgggtgtgct ggtcagtttc gttaaactga tggcttacgg
    12781 cagcattggg gtaggcagca gctttctccc ctggtgttta ttttgtgtcc tgcaactgcg
    12841 cgcttttcag tgcgttgatc gtcgctggtt atgggacgac atcgccccga tgccagaact
    12901 gcgccagccg ctaaaaccag gcgtcacggg gatacgtcag gggctgcgtt cgtgctcctg
    12961 ttgtacggca atccttcctg ctgatgaacc cgtgtgcccg cgttgtagta ccaaagggta
    13021 cgttcggcgt agaaacagcc tgcagtggac actcgcgctg cttgtaacgt ccatcatgct
    13081 gtatcttccg gctaatattt tgcccatcat ggtgacggat ttattaggct cgaagatgcc
    13141 gtcgacgatt ctcgctgggg tcattctgtt atggagcgaa ggctcttatc ccgtcgctgc
    13201 ggtgatcttt ctggccagta ttatggtgcc aacgttaaag atgatcgcca tcgcgtggct
    13261 gtgttgggat gccaaagggc atggcaagcg cgacagtgaa agaatgcatt tgatttatga
    13321 agttgttgag tttgtaggcc gctggtcgat gattgacgtt ttcgttatcg cggtgctctc
    13381 ggcgctggtg cgtatgggag gtttaatgag tatttatccg gcaatgggtg cattaatgtt
    13441 tgctttagtc gtcataatga caatgttttc tgctatgacg tttgacccgc gtttgtcgtg
    13501 ggatcgtcaa cctgaatcag agcatgagga gtcctgacag tatggaatct aataatgggg
    13561 aagccaaaat ccagaaagtg aagaactggt ctcccgtgtg gatatttcct atcgtcacgg
    13621 cgctcattgg ggcctgggtt cttttttatc attacagcca tcagggaccg gaagtgaccc
    13681 tgatcaccgc gaatgcggaa ggaattgaag gtggcaaaac caccattaaa agccgtagcg
    13741 ttgacgtcgg cgtggttgaa agcgccacac tggctgatga tttgacgcac gttgaaatca
    13801 aagcgcggct gaattccggt atggaaaaat tgctgcataa agacaccgtc ttttgggtgg
    13861 tgaaaccgca gattggtcgc gaagggatta gcggcctggg aacgctgctg tctggagttt
    13921 atatcgaact gcagccaggc gcgaaaggca gcaaaatgga taaatacgat ttgctggact
    13981 caccaccgtt ggccccgcct gatgcgaaag gtatccgtgt gattctcgat agcaaaaaag
    14041 ccgggcagct ctcgccagga gatccggtgc tgttccgtgg ctatcgggta ggttcggttg
    14101 aaaccagcac cttcgatacg caaaaacgca atatcagtta tcaactgttc atcaatgcac
    14161 cttatgaccg actggtgacc agcaatgttc gcttctggaa agatagtggc attgcggttg
    14221 atctgacgtc agcggggatg cgtgtggaga tgggctcatt gacaacgctg cttagtggcg
    14281 gtgttagctt tgatgtgccg gaaggtctgg atttagggca gccagtggca ccgaaaacag
    14341 ctttcgtttt gtatgatgat cagaagagca ttcaggattc gttgtacacc gatcacattg
    14401 attatctgat gttctttaaa gattcggtac gcggtctgca accgggagct ccggtagaat
    14461 tccggggtat tcgcctgggt accgtaagca aagtgccatt ctttgcgccg aatatgcgtc
    14521 agacatttaa cgatgattac cgtattccgg tactgattcg tatcgagcca gagcggctga
    14581 aaatgcagct tggcgaaaat gcggatgttg ttgagcacct tggcgaattg ttgaaacgtg
    14641 gtttacgcgg atcgctgaaa accggaaacc tggtcactgg tgcactgtat gttgatctcg
    14701 atttctatcc aaatacgcct gcaataaccg gtattcgtga atttaatggt tatcagatta
    14761 tcccgactgt tagcggcggc ttggcgcaaa tccagcaacg actgatggaa gcgttggata
    14821 agatcaacaa actgccattg aatccgatga ttgaacaggc aaccagtacg ctttctgaaa
    14881 gtcagcgcac aatgaaaaac ctgcaaacga cgctggatag catgaacaag atcctcgcca
    14941 gccagtcgat gcagcagttg ccgacggata tgcagtcaac gttgcgtgaa ttgaatcgca
    15001 gcatgcaggg cttccagcct ggctccgcag cctacaacaa gatggtggcg gatatgcagc
    15061 gccttgatca ggtgttgcga gaactgcaac cggtgctgaa aacgctcaat gagaagagta
    15121 acgcgctggt atttgaagcg aaggacaaaa aagatccaga gccgaagagg gcgaaacaat
    15181 gaaaaagtgg ctagtgacga ttgcagcact gtggctggcc ggatgcagct ccggcgaaat
    15241 taataaaaac tattaccagt tacctgtggt gcagagcggt acacaaagta ccgccagcca
    15301 gggcaatcgt ctgttatggg tagagcaggt cactgttcct gactatctgg cggggaatgg
    15361 tgtggtttat caaaccagtg atgtgaagta tgtgattgcc aacaacaact tgtgggccag
    15421 cccgttggat caacagttgc gcaacaccct ggttgccaac ctgagtacgc aactgcccgg
    15481 ctgggtggtt gcctcccagc ctctgggaag cgcccaggac acgctcaatg ttaccgtaac
    15541 ggagtttaac ggtcgctatg atggcaaggt cattgtcagt ggtgagtggc tgttgaacca
    15601 ccagggacaa ctgatcaaac gtccgttccg tctggaagga gtgcaaactc aggatggtta
    15661 cgatgagatg gttaaagtgc tggccggtgt ctggagtcag gaagccgctt ctattgcaca
    15721 agagataaag cgtctacctt aattataaag atttgtaaat ataaccgtct ccggtatgtt
    15781 gcctgaggcg gtttttttgt ttctaacgtg cggaaaaatt tgttcctctt cacatttttt
    15841 gtacaaccga catgcccgtg tagctcacaa atatgacagt ggcgtgaatt ttgcgcattg
    15901 acggcagtta tgattcgcgg tattgcttaa ctgtgattgc acatttagta atcactgttt
    15961 tcttttccac cagaaaccag tatgagggaa acgaggcatg aagagacaaa aacgagatcg
    16021 cctggaacgg gcacatcaac gtggttatca ggccggcatc gccggacgct caaaagaaat
    16081 gtgtccctat cagacgctga gtcaaaggtc acaatggctg ggaggctggc gagaagccat
    16141 ggcggacagg gtagtaatgg cctgattctg tctctttaaa aagaaacctc cgcattgcgg
    16201 aggtttcgcc ttttgatact ctgtctgatg ataatcagaa ggcagacgta tcctggaaca
    16261 gaccgacttt caggtcgctg gcggtataga tcagacgaca atcaaccagc acttcgccat
    16321 ctgccagacc cataatcaga cgacgattaa caatgcgttt aaagtgaata cggtaggtca
    16381 cttttttcgc tgtcggcagt acctgaccag tgaatttcac ttcgccaacg cccagcgcgc
    16441 ggcctttacc ttcgccgccc agccagccga ggtagaaccc taccagctgc cacattgcgt
    16501 ccaggcccag gcatcccggc ataaccggat cgccaatgaa gtggcatccg aagaaccaca
    16561 gatccggatt gatatccagt tctgcttcaa catacccttt gtcgaagtta ccacccgttt
    16621 cggtcatttt gaccacacgg tctatcatca gcatgttcgg tgctggcaat tgcgggcctt
    16681 tagcgccaaa cagttcaccg tgaccagagg caagaaggtc ttcttttgta taggattcgc
    16741 gtttatctac catgttctct gtaagcctta ttttattgaa gcacgcagga tagctaacac
    16801 gtgtacgctg aacaagtccg atcagttcgg aataaaccag ttcagccaac gtaatggcca
    16861 tggaaaacgg tgacgtcctt cctgttgcga tgcttgcgcg atacgttcct ggatggtttg
    16921 catcagcgtc gtttggcctt cgccatccca caccagattt aataataacg gcagtgcgtc
    16981 agtcacatcg tctactgccc agatggtgaa tttgccttct tctaccgctt tcaccagttc
    17041 actgtgaaga cttaaatggc gaacgttagc agtggggata atgacacctt gtttccccgt
    17101 taactcacgt tgctggcaaa tagcaaagaa gccttcgatt ttctcattta aaccaccgac
    17161 cggctggacg cggccgaact gatcgactga acctgtgata gcgatactct gattcactgg
    17221 cacatcagcg agggcgctta tcagggcgca gagttcagcc atcgaggcac tatcgccatc
    17281 cacttcactg tatgactgct caaatgtcag cgatgctgag aaggggatct gttgctcaag
    17341 ctgtagttcc gacatcagga acgcctgcat gatcatcatc cctttcgcat ggatattgcc
    17401 gccaagctcc gctttgcgtt cgatgtcggt gaattcacca tcgccaatat gcacaacgca
    17461 gctaatgcga gaaggttcgc caaaagcgcg tggatgaccc ggaaattcaa tgaccgaaag
    17521 ggcgttaatt tgcccgatgc gttcgccttc ggtttcaatc aggatttgct cctgaaggat
    17581 ctcatcctgc atacgttcag cgaggaaacc ttcgcgccat tcacgctgct gcagcattaa
    17641 gtttagctgc tcgccggaga aggtgtcgcc atcacacagg gaggcgacct ctttacactg
    17701 gcggaggatc cactgcgggc taagcggaag tgtttcttgt tcaccggtgt agcgtgctgc
    17761 ttcgcggata agtaccggcc aagcatccgc tcccggggca ggtaagtgat tatgtctggc
    17821 ggtaaatgtc acccagcgac accactggct tactgactcc gcatcgacaa tctgcagagt
    17881 atcttcaaat tcgctataaa tagcctgctc tgaaagctct ggctccatct cctggaaatc
    17941 agccaatgat tcgcgttcgc ctaccagaat gactttcagc ttcaatggca ttgaaggcac
    18001 agagacgggg agagggcgcg actcatcaaa cgcaacccag tcaaaacgct cgcggttaac
    18061 gatatttttc agccgcatcc acagcagagg ttgcgccagc agtgtacgca aagagatgat
    18121 gagaataccg ccatttgcct gatgcaccag accaggctgc agggtaatgt cgccattaaa
    18181 ctggcgcagg cagccaaaga gttgctccgc ttctacccag tcggcagcga caacttgcgt
    18241 taaagtcgca aaattatcat ctgcactcac tgcgtgacgt aagcggatgg agtggccgga
    18301 aacctcatag tgatcgccca ccagttggcc tgcatcgctt tgtagcgtac gcgcggcatc
    18361 ggcaataaga ttcagatact cagactcttc cggggcctta gccagcataa aagaggagga
    18421 tgctcgcgta tgcagcaact gctccagcgc aaattgcagt cgcggttgag tatcactgaa
    18481 taaaggatcg ttttcgtcaa tcaaatgtgg ctgagcaaat atttcctgat agctatcggt
    18541 atcaggaacc aggtcacgcc atgcaagttt cgtaatggtc aaagttgatg ttttttagtc
    18601 tgttgtcaaa gccgcgatta taccgtaacc ggcactacag cacacgtaga aagcaccgac
    18661 aatactcctg gctgggcgtt aaagctcaca ggatggagat tcttttcttc actggcctaa
    18721 aaagctgata ttctgtaaag agttacacgg taacattgag atcgctatga aatatcaaca
    18781 acttgaaaat cttgaaagcg gttggaaatg gaagtatctg gtgaagaagc atcgcgaagg
    18841 ggagttaatt acccgttaca tagaagccag tgccgcccag gaagccgtcg atgtgttgct
    18901 ctcgctcgaa aatgaacccg tgctggtaaa tggctggatt gacaagcata tgaatccgga
    18961 actggtcaat cgcatgaagc agaccattcg ggccagacgg aagcgccatt ttaatgcgga
    19021 acaccagcat acgcgcaaaa aatcgatcga tctggaattt attgtctggc aacgtctggc
    19081 tggtcttgca cagcgtcgcg gtaaaacgct ttctgaaacg attgttcagc tgattgaaga
    19141 tgcggaaaac aaagagaaat acgcgaataa aatgtcttct cttaagcagg atctgcaggc
    19201 attgctgggt aaggaataac tgacgaaagt cagttcaatt tactaaaggc aaaaaaaacc
    19261 ccgcagcagc ggggtttttc taccagacga taacttaagc ctgcggctga gttacaacgt
    19321 ctttgatacc tttaacttcg atctctacgc gacgatccgg agccaagcag tcgatcagtg
    19381 cagcacgctg tttcacgttg tcacaggtgt tgccagtaac cgggttggat tcgcccatac
    19441 cacgtgcgga gatcttgtct gccgggatac ctttggagat caggtaatca acaacagact
    19501 gagcacggcg ctcggacaga ccctggttgt aagcgtcaga accgatgcgg tcagtgtaac
    19561 ccagaacaac tacggaaccg tctttcggat ctaggttgct cagctggctg tacagctgat
    19621 ccagagcagc ctgaccttcc ggtttcaggg ttgctttgtt gaagttgaac agaacgtcag
    19681 acttcagagt gaagtgcttg gtctgtactt ccggtgccgg agctggagca actaccggag
    19741 ctgcttcgcc ctgaccgaaa cggtaggaaa cacccaggct cagcagaccg ttgtccggac
    19801 gagtaccgat ggtgtttgcg tcaccgatgt tgttggtcca ctggtattcc agacgggtag
    19861 cgatttcagg agtgatcgcg tactcaacac cacctgcgaa aaccggagaa acgccagtgt
    19921 cgtggtcttt aaaggatgcg ccaccaggta cgttagcctt ggtgtctgca cgccatacca
    19981 taccacccag acgagtgtag atgtccagat cgtcagtgat tgggtaaccc agtttagcgg
    20041 tcagctgaac gccctgagct ttgtatgcgc cgttgatgtt gtcgcctttg tacggcatac
    20101 gacctaacca gtcgtaaccc atttcaaaac caacatacgg gttaacctgg taaccaccaa
    20161 aagcacctgc acccagttgg ttttcgtggg tcggaccatt gttaggaata aaaccagtgt
    20221 catggtactg ggaccagccc agtttagcac cagtgtacca ggtgttatct ttcggagcgg
    20281 cctgcgctac ggtagcgaaa ccagccagtg ccactgcaat cgcgatagct gtctttttca
    20341 ttttttgcgc ctcgttatca tccaaaatac gccatgaata tctccaacga gataacacgg
    20401 ttaaatcctt caccggggga tctgctcaat attaactcta ccgatatctt cggcttatgc
    20461 cgagcacccc tggcgatgta aagtctacaa cgtagttgaa aacttacaag tgtgaactcc
    20521 gtcaggcata tgaaaaaaaa gtcttgtata aggtatgttt aatctttttg tcagcgacaa
    20581 tttacagaaa aaaaacgcgg aaaccgcttc agacaagcct tcgcaaggaa aattagtcac
    20641 gactgaaagc attggctggg cgacaaaaaa agttctagga ttaatcctaa atttacttaa
    20701 tgatacaaat tagagtgaat ttttagcccg gaaagttgtc tcgttgtgtg agaggatgcg
    20761 cttaccggac gcataataaa ccccatagcg ttaccttcat ttgccgcatc aacaagttca
    20821 gcatgctctt cttcagtcaa atcatctgtc aaccaaccga tcaccacact gtaattgccc
    20881 gtgcgtaaag cgcgaaccat tgactccact gtgtggcaag gggagagctg gctaatctgc
    20941 attactttcg ttaagggtag cccagatgcc tgaacccatt cccgactcag tttttgttgc
    21001 ggtgttaacc agagttgcca gcgcgattgc tgaccgagtt gctgtaacaa tggcaacagt
    21061 agaagttgcg tcatcatggg ctgatcttcg cgatagacaa cttcactgat aagcccggct
    21121 gtagtgtttt ccgtagagac acgcgcaatt ttacttgctg cggatgagaa cgacgaagaa
    21181 cgatgtgcat agcctgaagt gtacataatc aatccagccc ctgtgagtta ctgtatggat
    21241 gtgcagtaca tccagtgaca acaaagatca accctatttt cggaaagagc ctcgcaaatt
    21301 ttgtcgttgg tgacgggaaa acataaatta atcttgcccc ttaagaataa gttgcctatt
    21361 ttcgtagtta acggatccgt taatgtgaat cattctttta tgttatgatt ttaaaaggaa
    21421 ttttatgaaa agcctctcct ataagcggat ctataaatca caagaatacc tggcaacgtt
    21481 gggcacaatt gaataccgat cattgtttgg cagttacagc ctgaccgttg acgacacggt
    21541 gtttgcgatg gtttctgatg gtgagttgta tcttcgggct tgtgagcaaa gtgcacagta
    21601 ctgtgtaaaa catccgcctg tctggctgac atataaaaag tgtggccgat ccgttaccct
    21661 caattactat cgggttgatg aaagtctatg gcgaaatcaa ctgaagctgg tgcgtctgtc
    21721 gaaatattct ctcgatgcag cgctgaaaga gaaaagcacg cgcaataccc gggaaagact
    21781 gaaagatttg cccaatatgt cttttcatct ggaagcgatt ct
//
LOCUS       DAJTQQ010000062        21519 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000062 DAJTQQ010000000
VERSION     DAJTQQ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.062"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..423
                     /gene="tnpA"
                     /locus_tag="OO309_002592"
     CDS             <1..423
                     /gene="tnpA"
                     /locus_tag="OO309_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR5847696.1"
                     /translation="VFVTKYRRQIFDHDTTEKLRTYFSNVCADFAAELVEMDGEPDHV
                     HLLINYPPKLAISSLVNSLKGISNRLLRRDRPDIAVRYYYKGVLWSPGYFASSCEGAP
                     ISVIRQYIEQQQTPGQVENRALYPRPEGRGFTAHRIKD"
     gene            complement(462..809)
                     /gene="grxD"
                     /locus_tag="OO309_002593"
     CDS             complement(462..809)
                     /gene="grxD"
                     /locus_tag="OO309_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR5847697.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            1144..1971
                     /gene="mepH"
                     /locus_tag="OO309_002594"
     CDS             1144..1971
                     /gene="mepH"
                     /locus_tag="OO309_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR5847698.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            2099..2680
                     /gene="sodB"
                     /locus_tag="OO309_002595"
     CDS             2099..2680
                     /gene="sodB"
                     /locus_tag="OO309_002595"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR5847699.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(2826..3995)
                     /locus_tag="OO309_002596"
     CDS             complement(2826..3995)
                     /locus_tag="OO309_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5847700.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(4161..4250)
                     /gene="ynhF"
                     /locus_tag="OO309_002597"
     CDS             complement(4161..4250)
                     /gene="ynhF"
                     /locus_tag="OO309_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR5847701.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            4549..5574
                     /gene="purR"
                     /locus_tag="OO309_002598"
     CDS             4549..5574
                     /gene="purR"
                     /locus_tag="OO309_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR5847702.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(5571..6503)
                     /locus_tag="OO309_002599"
     CDS             complement(5571..6503)
                     /locus_tag="OO309_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5847703.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            6616..7827
                     /locus_tag="OO309_002600"
     CDS             6616..7827
                     /locus_tag="OO309_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR5847704.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            8118..9266
                     /gene="cfa"
                     /locus_tag="OO309_002601"
     CDS             8118..9266
                     /gene="cfa"
                     /locus_tag="OO309_002601"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR5847705.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(9306..9947)
                     /gene="ribE"
                     /locus_tag="OO309_002602"
     CDS             complement(9306..9947)
                     /gene="ribE"
                     /locus_tag="OO309_002602"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR5847706.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            10162..11535
                     /gene="mdtK"
                     /locus_tag="OO309_002603"
     CDS             10162..11535
                     /gene="mdtK"
                     /locus_tag="OO309_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR5847707.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(11576..12832)
                     /locus_tag="OO309_002604"
     CDS             complement(11576..12832)
                     /locus_tag="OO309_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR5847708.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            13140..13216
                     /locus_tag="OO309_002605"
     tRNA            13140..13216
                     /locus_tag="OO309_002605"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13174..13176,aa:Val,seq:gac)
     gene            13221..13297
                     /locus_tag="OO309_002606"
     tRNA            13221..13297
                     /locus_tag="OO309_002606"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13255..13257,aa:Val,seq:gac)
     gene            13405..13710
                     /locus_tag="OO309_002607"
     CDS             13405..13710
                     /locus_tag="OO309_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR5847709.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            13836..15258
                     /locus_tag="OO309_002608"
                     /pseudo
     CDS             13836..15258
                     /locus_tag="OO309_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(15270..16082)
                     /locus_tag="OO309_002609"
     CDS             complement(15270..16082)
                     /locus_tag="OO309_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5847710.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(16086..16871)
                     /locus_tag="OO309_002610"
     CDS             complement(16086..16871)
                     /locus_tag="OO309_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR5847711.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(16868..17536)
                     /locus_tag="OO309_002611"
     CDS             complement(16868..17536)
                     /locus_tag="OO309_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR5847712.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(17600..18238)
                     /locus_tag="OO309_002612"
     CDS             complement(17600..18238)
                     /locus_tag="OO309_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR5847713.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(18251..20352)
                     /locus_tag="OO309_002613"
                     /pseudo
     CDS             complement(18251..20352)
                     /locus_tag="OO309_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(20373..20999)
                     /locus_tag="OO309_002614"
     CDS             complement(20373..20999)
                     /locus_tag="OO309_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR5847714.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(21455..>21519)
                     /locus_tag="OO309_002615"
     CDS             complement(21455..>21519)
                     /locus_tag="OO309_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR5847715.1"
                     /translation="IQRSELEKQAMETVINALVK"
BASE COUNT         5386 a         5575 c         5008 g         5550 t
ORIGIN      
        1 gtctttgtca ccaaataccg acgccagatt tttgaccatg acacgacaga aaaactacgc
       61 acttactttt caaatgtatg tgctgatttt gcagctgaac tggttgaaat ggatggcgaa
      121 ccagatcacg tccatttgtt aattaactat cctcccaaac tggcgatatc cagtctggta
      181 aacagcctca aaggcatatc gaataggtta ctgcgacgag atcgaccaga tattgcagtc
      241 aggtattact acaaaggcgt tttgtggagt cctggctatt ttgccagtag ctgcgaaggt
      301 gcgccaatat ccgtcatccg ccagtacatt gaacaacagc aaacacctgg tcaggtggaa
      361 aaccgcgcct tatatccccg ccctgaagga cgaggtttta cggcgcaccg gataaaggac
      421 tgacggatgc ccgttcgcat ccgtcagtat tgcaggacgg attattccgc gtccggctct
      481 tcagacttgt atttagcggc agtttctttg atcagctgct gcagttcgcc acgctgatac
      541 atttcgatca cgatatcaca accgccgacc agctcgccgt caacccacag ttgcgggaag
      601 gtcggccagt tagcatattt cggcagttcc gcacgaatgt ccggattctg cagaatatca
      661 acataggcaa aacgttcgcc acatgcggca agcgcctgga ctgcctgggc agagaaaccg
      721 cagctcggca gtttcggtga acctttcatg tacagcagga tcgggttttc agcaatctgg
      781 cgttggattt tttcgatagt ggtgctcatt gtcttgcttc ctcaaacttc tttacggcag
      841 taatctgaca ttgtagcggg tcagtgcggc atcggaaaat aacattttca tcacgctttt
      901 gctattttat ccctttgctc tatttgttgc atttcaaata ttcgtttctt ctctgatgcg
      961 cggcaaactg gcgtatttat aaccattttt agctttcact gctgcatttt tttgcgctcg
     1021 ccaacgaaac gtatttttta acaataaaag ctattaactt tctcttcttc tatgcattag
     1081 aatcatcaag ttttgtaaat cagacgcagg catgatagac ctgccttaac agagggacgc
     1141 tcagtggcgc ggataaaccg tatttcgatc acgctctgtg ctttactttt taccaccctg
     1201 cctttaacgc ctatggccca tgcttcaaag caagccaggg agagttctgc taccactcat
     1261 atcaccaaaa aagcagataa aaagaaaagc acggcaacca ccaaaaagac ccagaaagcc
     1321 agccagaaaa cagcgaaaaa agccgccagt aaaagtatga ccaaaagcaa aaccgcttct
     1381 tctgttaaaa aatcttccat taccgcttct aaaaacgcca aaactcgcag caaacacgcc
     1441 gtcaataaaa cggcctcagc cagctttacc gaaaagtgta ccaaacgtaa gggctataaa
     1501 tcgcattgtg tgaaagtcaa aaatgccgcg tcaggaaccc ttgccgacgc gcacaaagcg
     1561 aaggtgcaaa aagccacgaa agtggcaatg aataaactga tgcagcaaat tggtaagcca
     1621 tatcgttggg gcggcagctc accgcgtacc ggttttgatt gcagcggcct ggtttattac
     1681 gcttataaag atttggtgaa aattcgtatt ccgcgtacgg cgaatgaaat gtatcacctg
     1741 cgtgatgcag gacctatcga acgtagtgag ctgaaaaacg gcgacctggt ctttttccgt
     1801 actcagggac gcggtacagc cgatcatgtc ggcgtgtatg tcggcaacgg caaatttatt
     1861 cagtcaccgc gcacaggtca ggaaattcaa atcacttctc tcagtgaaga ctactggcag
     1921 cgccactatg ttggcgctcg tcgggtaatg accccaaaaa cacttcgcta aaactttacc
     1981 ctgttgttac ggcaacaggg taagttcatc ttttgtctca ccttttaatt tgctacccta
     2041 tccatacgca caataaggct attgtacgta tgcaaattaa taataaagga gagtagcaat
     2101 gtcattcgaa ttacctgcac taccatatgc taaagatgct ctggcaccgc acatttctgc
     2161 ggaaactatc gagtatcact acggcaagca tcatcagact tatgtcacta acctgaacaa
     2221 cctgattaaa ggtaccgcgt ttgaaggtaa atcactggaa gagattattc gcagctctga
     2281 aggtggcgta ttcaacaacg cagctcaggt ctggaaccat actttctact ggaactgcct
     2341 ggcaccgaac gccggtggcg aaccgactgg aaaagtcgct gaagctatcg ccgcatcttt
     2401 tggcagcttt gccgatttca aagcgcagtt tactgatgca gcgatcaaaa actttggttc
     2461 tggctggacc tggctggtga aaaacagcga tggcaaactg gctatcgttt caacctctaa
     2521 cgcgggtact ccgctgacca ccgatgcgac tccgctgctg accgttgatg tctgggaaca
     2581 cgcttattac atcgactatc gcaatgcacg tcctggctat ctggagcact tctgggcgct
     2641 ggtgaactgg gaattcgtag cgaaaaatct cgctgcataa taactgatgg caaatgcagc
     2701 attgcctgat gcgcttcgct taccatgcca acgcggatga ggcgttaaca gcgcattcgg
     2761 caagataaat cgcacgttgt cagcaactgt aacgcagaag gttatccttc tgcgtttttg
     2821 tttaattagc tgttagcaac gcaaactgtt tcaggttgtt ttctggctga cataaacacc
     2881 agcaataatg ccagtcccgc gacaatcgcc cccgtcaccg gcacaaagct gtatcccagc
     2941 ccagcggaaa ttaccgcacc accagcagct gctcccagcg catttccaag attaaaggca
     3001 ccaatattga ctgatgaaga cagacctggc gcttcactgg cgacatgcat cacgcgcatc
     3061 tgtaacggag gtacaaccgc aaaggttgct gcgccccaca ccaccatgct aatagctgcg
     3121 ccgaactcat tgcgggccag gaacgggatt gccagcataa tcaccatcag cagcaacaaa
     3181 aagcctttca acgtgccgtt aactgaacga tctgccagtt tgccgccgag atagttaccg
     3241 atagagaatc cgacaccaat cagcaccagc attgccgtga cgaacaccgg tgttgcgtgg
     3301 gtaatacttt gcagtaccgg agagatatag gtgtagaggg taaacattgc accagctccc
     3361 agtaccgtcg tcagcaatgc agacagcacc tgcggacgca ttaataccgc cagctctttt
     3421 ttcacttcag gtcgtgcccc tgtaccacct ttaggtaatg agaagaacag acttaccatt
     3481 gaaatcactc ccagccccgc cgttgccaga aatgacatcc gccagccgat ggtttcaccc
     3541 aaccaggtcg ccgccggcac gccaccgata tttgccaggg ttaaccccat aaacatagtg
     3601 gcaactgcgc tggcctgttt atgttttggc accacgcttg cggccacgac tgaacccaaa
     3661 ccaaaaaatg ctccgtgatt caggctggtc aaaatacgtg aaagcatcag ggtcatataa
     3721 tccggcgcga tggcggaaag aacgttgcct agcgtgaaaa ttgccatcag gaaaatcaac
     3781 gcactgcggc gggcacgatg agaaagtaga agcgtcatca gcggcgcgcc caccattacg
     3841 ccaactgcat aggcactgat taacattccg gcagcgggaa tcgagacatc cacaccgcgc
     3901 gcaatgacgg gcaacaagcc cattggcgag aactccgttg tcccgatacc aaacgcgcca
     3961 atcgccagcg ccagcaacgg atagttaatt ttcatgcctt atctccacct cttcgcgtca
     4021 ttacgcgata ttcattaaag tggcgaaagc atgacagcaa tcacaaaaaa atgaaaataa
     4081 caaaaagaga aaacactttt gccattttgc taacaaacag gaaggagatg cgagggagaa
     4141 cgcgctccct cgagaggaaa tcagtgcagc gcggcagtca aacccacggc tacgatcaaa
     4201 ccgaggacga taaccgttgt taccagtgaa aatttaaggt cggtgctcat caagttttct
     4261 ccttttttat taccacacaa aaagtgatat tacgcatttt tacacactgt gatgaaaaaa
     4321 tctcccgtca tttataatga taagtgtttt taccacttcc ccttttcgtc aagatcggcc
     4381 aaaattccac gcttacacta tttgcgtact ggccattgac cccttcctga cgctccgtgt
     4441 cgtttttccg gcgtaccgca acacttttgt tgtgcgtaag gtgtgtaaag gcaaacgttt
     4501 accttgcgat tttgcaggag ctgaagttag ggtctggagt gaaatggaat ggcaacaata
     4561 aaagatgtag cgaaacgagc aaacgtttcc actacaactg tgtcacacgt gatcaacaaa
     4621 acacgtttcg tcgctgaaga aacgcgcaac gccgtgtggg cagcgattaa agaattacac
     4681 tactccccta gcgcggtggc gcgtagcctg aaggttaacc acaccaagtc tatcggtttg
     4741 ctggcgacca gcagcgaagc ggcctatttt gccgagatca ttgaagcagt tgaaaaaaat
     4801 tgcttccaga aaggttacac cctgattctg ggtaatgcgt ggaacaatct tgagaaacag
     4861 cgggcttatc tgtcgatgat ggcgcaaaaa cgcgtcgatg gtctgctggt gatgtgttct
     4921 gagtacccag agccgttgct ggcgatgctg gaagagtatc gccatatccc aatggtggtg
     4981 atggactggg gtgaagcaaa agctgacttc accgatgcgg tcattgataa cgcgttcgaa
     5041 ggcggctaca tggccgggcg ttatctgatt gaacgcggtc accgcgaaat cggcgtcatc
     5101 cccggcccgc tggaacgtaa caccggcgca ggccgccttg ccggttttat gaaggcgatg
     5161 gaagaagcga tgatcaaggt gccggaaagc tggattgtgc agggtgactt tgaacctgaa
     5221 tccggttatc gcgccatgca gcaaatcctg tcgcagccgc atcgccctac tgccgtcttc
     5281 tgtggtggcg atatcatggc aatgggcgca ctttgtgctg ctgatgaaat gggcctgcgc
     5341 gtcccgcagg atgtttcgct gatcggttat gataacgtgc gcaacgcgcg ctattttacg
     5401 ccggcgctga ccacgatcca tcagccaaaa gattcgctgg gtgaaacagc gttcaacatg
     5461 ctgttggatc gtatcgtcaa caaacgtgaa gaaccgcagt ccattgaagt gcatccgcgt
     5521 ttgattgaac gccgctccgt ggctgacggc ccgttccgcg actatcgtcg ttaatcaccc
     5581 gttgcgggag tctcttccgg ctcccgcagc cactccttat tcagcgtctc actatcgctg
     5641 agatactcaa gcagccaggt taacgcaggc gacatatcat tttgctgcca tgtcagacaa
     5701 catgccgaat ccgggaaggg gttttccagt tcaagtgcta cccacttccc ctcattaagc
     5761 cacggtttgg cgaaatgtgt tggcaccatc cctatgcata atcctgccga gatacaggtt
     5821 gccgatgatt cccagtcagg tacgacaagt cttttttggt tatccagcaa ccaggtaata
     5881 cgtttaggta gcgttcgcga ggtgtcttcg cgcaccaacg acggccagtt gcgcaacgta
     5941 tcatcgctga acgggccatc catcaacgcc agcgggtggt gactggcaac aacgcaactc
     6001 cagcttagca tccccatatc ccggaaggca taacgaccgc ctaccggaat cgcgcgtgtt
     6061 gcgccaattg ccagttccac gcgcccgtcg gaaagcgcat cccagacacc gttgaacact
     6121 tcctgaaaga caagaagttc gacatcatca aaatggcgat aaaaatcaac gatcatctgc
     6181 cgtgtacgtt ctggcctgac aatattatcc actgcgatag ctaactgacc gcgccagccg
     6241 ttcgctatct gctgacattg ctggcgggtg atctgcattt ttttgacaac agagcgccct
     6301 tctttgagaa accacgctcc agcagcggtc agctccacat cacggtgccg tcgttcaaag
     6361 agcggtaccg ccagccactc ttccagctga cgcacggtat agctgaccgc agaaggaaca
     6421 cgatgcagct cctgtgccgc agcgctaaaa ctaccattac gcgctaccgc atcaacaact
     6481 tcgagtgaat attctgacca catagtctgc ctgcaaaatt tttgaaacca gtcatcaaat
     6541 attaccgttt cacaacacta atttcactcc ctacactttg cggcggtgtt taattgagag
     6601 atttagagaa tatacatgca acctgggaaa agatttttag tctggctggc gggtttgagc
     6661 gtactcggtt ttctggcaac cgatatgtat ctgcctgctt tcgccgccat acaggccgac
     6721 ctgcaaacgc ctgcgtctgc tgtcagtgcc agccttagtc tgttccttgc cggttttgcc
     6781 gcagcccagc ttctgtgggg gccactctcc gaccgttatg gtcgtaaacc ggtgttatta
     6841 atcggcctga caatttttgc gttaggtagt ctggggatgc tgtgggtaga aaacgccgcc
     6901 acgctgctgg tattgcgttt tgtacaggct gtgggggtct gcgccgcggc ggttatctgg
     6961 caagcattag tgacagatta ttatccttca cagaaagtca accgtatttt tgcgaccatc
     7021 atgccgctgg tgggtctgtc tccggcactg gctcccctgt taggaagctg gctgctggtc
     7081 catttttcct ggcaggcgat tttcgccacc ctgtttgcca ttaccgtggt gctgattctg
     7141 cctattttct ggctcaaacc cacgacgaag gcccgtaaca atagtcagga tggtctgacc
     7201 tttaccgacc tgctacgttc taaaacctat cgcggcaacg tgctgatata cgcagcctgt
     7261 tcagccagtt tttttgcatg gctgaccggt tcaccgttca tccttagtga aatgggctac
     7321 agcccggcag ttattggttt aagttatgtc ccgcaaacta tcgcgtttct gattggtggt
     7381 tatggctgtc gcgccgcact gcagaaatgg caaggcaagc agttattacc gtggttgctg
     7441 gtgctgtttg ctgtcagcgt cattgcgacc tgggctgcgg gcttcattag ccatgtgtcg
     7501 ctggtcgaaa tcctgatccc attctgtgtg atggcgattg ccaatggcgc gatctaccct
     7561 attgttgtcg cccaggcgct gcgtcccttc ccacatgcaa ccggtcgcgc cgcagcgttg
     7621 cagaacactc tgcaactggg tctgtgcttc ctcgcaagtc tggtagtttc ctggcttatt
     7681 agtatcagca cgccattgct caccaccacc agcgtgatgt tatcaacagt agtgctggtc
     7741 gcgctgggtt acatgatgca acgttgtgaa gaagttgact gcccgaatca tggcaatgcc
     7801 gaagtcgctc atagcgaatc acactgatct atatcgatat acttatactt aggctgctaa
     7861 caaaattttg ttgtatgatt gaaattagcg gcctatacta atttcgagtt gttaaagcta
     7921 cgataaatat tatgttttta cggggacagg atcgttcccg actcactatg gatggtcatt
     7981 tcggcaaggg ttcctccttt ccctctgttc tacgtcggat tatagactcg cggttttttc
     8041 tgcgagattt ctcacaaagc ccaaaaagcg tctacgctgt tttaaggttc tgatcaccga
     8101 ccagtgatgg agaaactatg agttcatcgt gtatagaaga agtcagtgta ccggatgaca
     8161 actggtaccg tatcgccaac gaattactta gccgtgccgg tatagccatt aacggttctg
     8221 ccccggcgga tattcgtgtg aaaaaccccg atttttttaa acgcgttctg caggaaggct
     8281 ctttggggtt aggcgaaagt tatatggatg gctggtggga atgtgaccga ctggatatgt
     8341 tttttagcaa agtcttacgc gcaggtctcg agaaccaact cccccatcat ttcaaagaca
     8401 cgctgcgtat tgccggcgct cgtctcttca atctgcagag taaaaaacgt gcctggatag
     8461 tcggcaagga gcattacgat ttgggtaatg acttgttcag ccgcatgctt gatcccttca
     8521 tgcaatattc ctgcgcttac tggaaagatg ccgataatct ggaatctgcc cagcaggcga
     8581 agctcaaaat gatttgtgaa aaattgcagt taaaaccagg gatgcgcgta ctggatattg
     8641 gctgcggctg gggcggacta gcgcactaca tggcatctaa ctatgacgta agcgtggtcg
     8701 gcgtcaccat ttctgccgaa cagcaaaaaa tggctcagga acgctgtaaa ggcctggatg
     8761 tcaccatttt gctgcaagat tatcgtgacc tgaacgacca gtttgatcgt attgtttctg
     8821 tggggatgtt cgagcacgtc ggaccgaaaa attacgatac ctattttgcg gtggtggatc
     8881 gtaatttgaa accggaaggt atattcctgc tccatactat cggttcgaaa aaaaccgatc
     8941 tgaatgttga tccctggatt aataaatata tttttccgaa cggttgcctg ccctctgtac
     9001 gccagattgc tcaatccagc gaaccccact ttgtgatgga agactggcat aacttcggtg
     9061 ctgattacga tactacgttg atggcgtggt atgaacgatt cctcgccgca tggccagaaa
     9121 ttgcggataa ctacagtgaa cgctttaaac ggatgtttac ctattatctg aatgcctgcg
     9181 caggtgcttt ccgcgcccgt gatattcagc tctggcaggt ggtgttctca cgcggtgttg
     9241 aaaacggcct tcgagtcgct cgctaaaggc tattctatcg ccccctcttc gggggcgagt
     9301 tcagatcagg cttctgtacc tggttgattc atggcatttt ctcgtgccgc cagcacacgt
     9361 tctaccgtat ctaccactgc ctgagtttgt ggatcgattt caatgttgac gcgtgcgcca
     9421 agttttttct tcccaagagt cgtgcgttcc agtgtttccg gaattaaatg gacgcaaaaa
     9481 cgcgttggcg tgacttcgcc gacggtcagg ctaataccgt cgatgccaat aaatcctttg
     9541 tacaggatat atttcatcaa ctgactatcc tggactttaa accagatctg gcgattattt
     9601 tccgaggtta atattttcgc cacttcagcg gtggtcataa tatgacctga cattaagtgt
     9661 ccgccaattt catcactgaa tttcgccgca cgctcaacgt ttacccaatc ccccactttt
     9721 aaatcgccaa gattggtaat gcgtaacgtt tctttcatca ggtcaaaact gacatggttg
     9781 ccgttaattt ccgtcacggt caggcagcaa ccgttatgcg ccacggaagc accggtttcc
     9841 aggccgtcca gcatgtggtc gggtaactcc accacatgcg tacgaaaatt tggtttctcg
     9901 tcaatcgaca ccagttttgc ggtgccctgt acaatccccg taaacatact tacaactcct
     9961 gaaatcagtt aagacattct gttcagcaca atagcaggtg gaaaacgccc ttaccagtga
    10021 aggggtaaga atggctattt tttcactgga gaattaataa atcctcgcta caatagactg
    10081 aatttcccct gcttcttctt tttgctgccc attcaggcgg ctttttagtc tctcatataa
    10141 ctacaaataa aaggtgttca cgtgcagaag tatatcagtg aagcgcgtct gttattagca
    10201 ttagcaatcc cggtgattct cgcgcaaatc gcccaaactg cgatgggttt tgtcgatacc
    10261 gtgatggcgg gcggctatag tgccaccgac atggcggcgg tcgctatcgg tacttctatc
    10321 tggcttccgg cgatcctctt tggtcacgga ctgctgctgg cattaacgcc ggttatcgcg
    10381 caattaaatg gttccggtcg acgtgagcgc attgcgcatc aggtgcgaca aggtttctgg
    10441 ctggcaggtt ttgtttccgt tctcattatg ctggtgctgt ggaatgcagg ttacattatc
    10501 cgctacatgg aaaacatcga tccggctctg gcggacaaag ccgtgggtta tctgcgtgcg
    10561 ttgttgtggg gcgcgccggg atatctgttc ttccaggttg cccgtaacca atgtgaaggt
    10621 ctggcaaaaa ccaagccggg tatggtaatg ggctttatcg gcctgctggt gaacatcccg
    10681 gtgaactata tctttattta tggtcatttc ggtatgcctg agctcggtgg cgttggttgt
    10741 ggcgtggcta ctgcggcggt gtattgggtc atgttccttg ccatggtttc ttacattaaa
    10801 cgcgcccgct ccatgcgcga tattcgtaac gaaaaaggca ccgctaagcc cgagcctgcg
    10861 gttatgaaac gactgattca actcggtttg ccgattgcgc tggcactgtt ctttgaagtg
    10921 acactgtttg ccgtcgtggc tctgttagtg tctccgctcg gtattgttga tgtcgcagga
    10981 caccagattg ccctgaactt tagttcactg atgtttgtgc ttccaatgtc gctggcggca
    11041 gcggtaacta tccgcgtagg ttatcgtctg ggtcagggct caacgctgga tgcgcaaacc
    11101 gctgcgcgga ccgggcttat ggtgggtgtc tgtatggcaa ccctgacggc cattttcacg
    11161 gtttcactgc gggagcaaat cgccctgttg tacaacgaca atcccgaggt tgtaacgctg
    11221 gctgcgcatt tgatgttgct ggcggcggta tatcagattt ctgactcaat ccaggtgatt
    11281 ggcagtggga ttttgcgtgg ttataaagat acgcgttcca ttttctatat cacctttaca
    11341 gcttactggg tgctgggctt gccaagcggc tatattctgg cactgaccga tctggtcgtt
    11401 gaacctatgg ggccagcagg cttctggata ggctttatta tcggcctgac atcggcagcc
    11461 attatgatga tgctgcgtat gcggttcctg caacgtatgc cgtcagccat cattctgcaa
    11521 cgagcgtccc gctaataaag acaaggcgca accttcacgg gttgcgcctg tgtttttacg
    11581 caggctggag cgttgcgcca atcccgtctt cgtctggctg taatttcaga gcgttacaca
    11641 gagtttgccg actggcatct gttatctcaa cgcgtatatc cccgttaact tcccctttca
    11701 cattgatgaa gtcaggtgaa atttctttcg cactggtttg caggcaaatc gctccctctc
    11761 cgcgcaggcg tttcactttc agttgcacat gcgttgccgc accagtgatc agccacagtt
    11821 ttccgccatt cagattgatg ttaccaacaa tgaaagtgac gctgtcacct tttaccgtac
    11881 tgccgtcgag aatcaacaca ccgccctgct gtacagtaat cgttgattgt tgcagctctg
    11941 cgccataacc aataatcatt gaagcgccgt ccagtaatgc aacactcccg gtcagttcaa
    12001 ccggtgcgcg atcttcctct acgacaaaat tttcgtccgc gctggtgggt gacaacagga
    12061 tcactgttcc accttgtatt accgcatttt ccagcgtacc ggcatgcact tcagcccgcc
    12121 cccccacatt caccgacagg ttttgcgtct taccggaact gtaaagctga aggccatccg
    12181 tccccagacg ataaatggat ccattctcaa caatagtgtc agagtcttca ccaccgagat
    12241 tattcatggt gccaccagca agcaacgtat cactggcttt cccggccagc ataaaaacgc
    12301 cgccctgatt aatctcacaa ttgctggctt ctccacttgg atacaccaac aattcaccac
    12361 cttcatctac cacgaccgct gaggttgtcc cattaaccaa caggccgccc tcttgatcga
    12421 gaatgatttt ttccgcgcga tgtccttcca gtacacgcag gttaccgcca ttttccagca
    12481 acaaaccgca ggcataaccc tgatcaacgc tgaattcacc ttcgggatgg cggccattaa
    12541 ccgtagcgag cgtagagaga ctaattgcgc ctccctcgga ctgaacaata tctgtggcaa
    12601 tggcaccgtc gctgaccgat agcgtgccat tgttaccgta ctgtgtgcat ttggcgatcc
    12661 caccggttgc cactttcaac cagccagccc ccgtgaccac ggtattttcg gctcttccgc
    12721 cagcttcaac aatcaactcg ccttcagtaa gttgcgtcgc gccaaggacc tcgccggtct
    12781 taacaatttg cacattcccg tcgctcatca agtttttcgc atcagatccc atgatttatt
    12841 cctttgctgc atctgtgtgc ctttattgct acctaagtgt aaaggctacg gaggatttat
    12901 ccacgacaga tttgagatgg tggcaaacaa ctctgtttaa actctgatac acgaattatt
    12961 gggttgtatc agatgtaaat gcgatcctga ataaaaatcg cccttgcaaa tcaacaaaat
    13021 atagccaatt tgcttaaaag ctctgcaaac ggtcgaaata gcgcagaaaa ttacgttttg
    13081 cctcttgcca cctttccact ctgccgctaa tattcgtccc cgttgtcacc tacaacgttg
    13141 cgttcatagc tcagttggtt agagcaccac cttgacatgg tgggggtcgt tggttcgagt
    13201 ccaattgaac gcaccatcct gcgtccgtag ctcagttggt tagagcacca ccttgacatg
    13261 gtgggggtcg gtggttcgag tccactcgga cgcaccagat tttcttaatc tggtcttttc
    13321 ctttttccct ctgtttcttc tctgtatcca atacgttaaa agatttacac tgtcttcgta
    13381 tgcgttatca gaaggagaat cgctatggca actttgttac aacttcattt tgcttttaat
    13441 ggcccatttg gcgacgcaat ggctgagcag cttaaaccac ttgctgagtc gattaatcag
    13501 gaacctggtt ttctgtggaa ggtatggaca gaaagtgaaa agaaccacga agccggtggg
    13561 atctaccttt tcactgatga aaaaagcgcc cttgcctatc tggaaaaaca tactgcccga
    13621 ctgaaaaatc tcggcgttga ggaagttgtc gccaaagttt tcgatgtcaa tgagccactt
    13681 agtcaaatca atcaggcaaa actcgcctga cagaatttaa tcaagggcgg ttagcgccct
    13741 tttcatccct gtctgaaatt tctcaaattc taaaaatctc aaccaaactt atctgataac
    13801 actaaattcg aaagaatgcg tacaggtaag taacaatgaa aaaaattgct attgtgggtg
    13861 ccgggcctac ggggatctac accttattct cgcttctaca gcaacaaact ccactttcta
    13921 tttctatctt cgagcaggct gacgaggccg gtgtcgggat gccatacagt gatgaggaaa
    13981 actcaaaaat gatgctggca aatattgcca gtattgaaat accgccgatt aattgtacgt
    14041 atctcgaatg gctacaaaag caagaagcca gccatctcca gcgttatggc gttaaaaaag
    14101 aaaccttgca cgatcgtcag tttttaccgc gaattctgct gggcgaatat ttccgcgatc
    14161 aatttttacg attagtagac caggcacgac agcaaaaatt tgcagtggct gtttatgaat
    14221 catgccaggt taccgatctg caaattacaa atgctggcgt catgctcgct acaaatcagg
    14281 atttacccag cgagacgttt gatttagcgg tgattgccac gggtcacgtc tggcctgatg
    14341 aagaagaagc aaccagaacg tattttccca gcccgtggtc aggcctgatg gaagcaaagg
    14401 tcgatgcgtg taacgtgggt attatgggaa catccttgag cggactggat gcggcaatgg
    14461 cagtggctat tcagcatggt tcgttcattg aagatgataa acaacacgtc gtttttcacc
    14521 gcgataacgc aagtgaaaag ctaaatatta cgttgatgtc gcgcacgggt attttacccg
    14581 aagccgattt ctattgccct attccctacg agcccttaca catcgtcacc gatcaggcat
    14641 taaatgctga gattcaaaaa ggtgaagagg gccttttgga tcgggtattt agattgatag
    14701 tagaggaaat caagtttgct gatccagact ggagccaacg catagcctta gagagcctga
    14761 atgtcgattc ctttgctcaa gcctggtttg ccgagcgcaa acaacgcgac ccatttgact
    14821 gggcagaaaa aaatctccag gaagtcgaac gcaataaacg agaaaaacat actgttccct
    14881 ggcgttatgt cattctgcgc ctgcatgaag ccgtacagga aattgttcct catctgaatg
    14941 aacacgacca taaacggttc agtaaaggcc ttgccagggt tttcatcgat aattatgcgg
    15001 caatcccttc agagtctatt cgtcgactac tggccttacg tgaagcgggg atctgaaatc
    15061 cctgatgttg gcgaagatta tacgttacag caacccgaag atattcgtgg acgcgtagcg
    15121 ttcggcgcgt tgccctggtt gatgcacgac cagcctttcg ttcagggact tacggcatgt
    15181 gcagaaattg gtgaggcgat ggctcgggcg gtcgtaaagc ctgcatcccg tgctcgtcgg
    15241 cgtctttcgt ttgattaaag gtaaagctat cagcactcaa caaactgatg ctgttcatta
    15301 agacgatagc ggactccggt tcgaatattg ttttcacctt caatggcgac ggcgaagcga
    15361 actctttcgc cgtcatgata tgccagtgcg gcgcttcccc ctagcccgag aataattgag
    15421 tcgaccacgc ccgtgctcac taccgttgaa ttttcaccgc tggcaatgat gtgtacattg
    15481 tcgccgctgt tggcaattct ggcgtttgcg ccaaagctgg cgatttgcac caggtcacca
    15541 ttactggcga catgacaccg ttcacccaat gtacaaacgc gtactcgcat tccggtattc
    15601 gcaatccgac tactatctcc agcgctgctt atccgcgaag aattaccggt tacagcaacc
    15661 cgtgcgcgtt caccggagct gccaatgtga ctgttaaagc cgacacttcc aatccgcacg
    15721 ctataaccca tactggcaat ttgcgcggca tacccagcgg ttgcgattcg ggcgttatcc
    15781 tcacaacagc cgatgcgagc atgatcgtcg ctgcgggcaa actgactgca aaatggtatc
    15841 tctccggggt ctgtggccag tttttgcata gaggaaactt cctgatgcgc gaagttttca
    15901 tccgccagcc attttttcca cgcatactca accagactct ccgcccagtc cgtatatcct
    15961 tcctcaatca gtgcatgacg aatatcggca taactaccgc cgcgtgggaa atggcgcagg
    16021 aaccagcgat acatcaccgc tccgatacgc cattctcgca ggtctgctcg tgtaatgatc
    16081 attcgttagt gccggtgata gccatcgacc atgcttttaa aggtttcgtg tggcgtacgc
    16141 cccgtggtgc aaagataaag atgaccgaag ataaaaaaga ggcttataaa tgccagagca
    16201 aaatgcgcct gcaataacca gtatcttacg ccaggaaaca catctcccac ggcttgcgga
    16261 tagagacaca acagccccgt caatagtaac aacggcagca atccatacat gacaccaaca
    16321 taggcgacct gctgtaaggg attaaattta gactgtgttg ttgccgggaa aggatgttct
    16381 tccccctgca taatgccaaa caaataaaat cgcgtttgtt ttgccgctcg ttccagccac
    16441 ccctgacgac gaatgcgata gtggtgacca ttatccccaa cggcattgat cagcacaaag
    16501 ccgagccagc atgccagtaa caaaaatccg caaacttcat gcaccgcaac cagactttta
    16561 accgcagttg cgcccaccat cgcaaagtga tttatcagcc cacttgccag caacaataca
    16621 aagagtaacg cattcgacca gtgccataat ctgaccgctt tgctgtaaag ataaaccttc
    16681 tcaccatgat cggtggctga tttcttcacg ccacgagcac gaagcaaggc gtgcagcccc
    16741 agcaccaacc acatcccaac cagcaatact ccggcaatga tcaaccacac cggccaaaat
    16801 tcgggagtga actgtggcac atagttcgct aactggctct gaaactgttc agcatgttgc
    16861 gacgggttca tacattttcc tttttaatca aatgttgacc gaaccggcga tacagatgcg
    16921 gttttccggc accaggtagc tgatattgat agtatttatt atcctgcaac cacgcctgaa
    16981 tttctgggct atcttcgcga ccaaagatca atgcgtgttc cggacaggcg ctgacgcaaa
    17041 tgggcggaaa gccttttgcc agtcgggact cagcgcagaa atcacattta tctgccactt
    17101 tagtgacagg gttaaggtag cgaaccaggt acggacaagc gccaatgcaa taactgcagc
    17161 cgatgcactg cgatttttct acccgcacga tcccctgctc atcacgccac gatgcccccg
    17221 tcgggcatac gtcgatgcac ggagcatctt cgcagtgctg acatgactgg cgaaaaaagt
    17281 gatactgcgt ttcgttgtcg ttatcggtga ctggaatgtg tgctatcgat aaacggcttc
    17341 cctgagcggg gacgtggtta gttttacggc atgcgcgggc gcaaatatta cagccattgc
    17401 accgtgactc gtcatgaatc atggcatacc gaacttcctt ttcttgcctc gtgttcgcta
    17461 acagagaaga cgcagaacca gtaaaaaaga ttactgttcc catccccaga acaaatttgc
    17521 gtcgagtgaa ggacatctga ttatccccta atgcagtggc tgcaactgtc caccctatcc
    17581 atgacatctc cctgaaattt caggggttct gaaactgctc tacctcgaac cattcgcata
    17641 agccataagc cttgccatag gtcataaatc gctcgctaaa caagtacaac accgccgtcg
    17701 atgactccac ctgccgtata tcggcatatt caggtgcaac atccatcgcg gcgatcgcgg
    17761 cttcaatctg cgcttcctgg aagtaatatg gcgcttgcat cagcatggcg actttgtaag
    17821 gacgaggata cgtctggcac tcaaaacgaa cggcgtgtgc aatggcgcga caaatgtcct
    17881 gctccacaac ttgcaaagac atcgccgcgt agttttcact catggcctgc gttgagtaat
    17941 agtaatcatc ctgacttccc gtcatacaag cgatatccgc gtagtctccg ttctgcttaa
    18001 tgctttccag caaaactgac agttcctctt ctgcaaccga atagggtggc gagagaaaca
    18061 cggtgcgcgc caccagctgc ccagccgcag aatgctggcg aatgaactca gccaggattt
    18121 cagcaggttc tggttcggtt accgtatcaa cggggtgacg catcccctgc aaccattgcc
    18181 gcttcgcctc gtcaacttcg ctgactttcg ctaacggcaa ttcatcctga tggttcatct
    18241 tacccattcc ttacgcaggc agtagattgt gtgctgccag atcggcggca atatcttcca
    18301 gccccagacg ctgcaatgtt tcgcgggttg gacaaccaag ctgtgggtcc cagcccattt
    18361 ctttgtagaa catcgtgagc gaagcatgca tatcgtcacg atccattttg tcagtacctt
    18421 cagtaaagac cgggatctgc ggatccttgt cgaaaaccca ggaacagata agatcgtgtt
    18481 cattacgcat atctttggtt tgcatcggtt ttaccgtgta ggcacgatgc aacgtaaaga
    18541 tacgctctgc agctaaatct aatttttcct gagtcatctc ttcgccggtg atcgctttga
    18601 agaatttggc ttcaagcgcc agatcgcccc ggtaattacg gcttttcagc ggcgaaacgg
    18661 tcattggcca gacccagttg cacagagtga cggcgttatg caaacagacc cgcaacagcg
    18721 accactttgc atatttaatt tttgcgtcat tgattggcgt gtagtttttg gtttcatcgt
    18781 aagcatcttc agaaccaaaa agttctttcg ccacttcacg ttgcagtttc aatggcaagc
    18841 cggtaccaat aaagttgata tgggtatgcg tcatgcagtc acggttgaac atacacttaa
    18901 caatggaacc gacctgcgct gacgcttcat tggcatggtg aaccggatag ccaaacggcg
    18961 accagagttt atttttcgcg tagccccagt actctttacc caaattccag cgttctgcga
    19021 tggcatatga accatcagcc aggtgactca gctcacccac acgatgcgcc agacggtagt
    19081 aaaaatcttt aatgaagtta acgtcacccg cttccagttg atcccagcga atttctgcat
    19141 actcttcagc tggcagaaca cgcttgaaca cacctttgct gtaacaataa gtaaagtcgc
    19201 gatgcaactg cccgtagtta caccataggc cgtagtcatc gaacaaattc agaccaacca
    19261 ggttaccaat cacacgacca tcgtctttat cttcaaaatc tttcggcccg ttcgggaaga
    19321 tggtggtatg gacaaagttt gctacacaag tgttaccacc tgtgctgggc acgccaaact
    19381 ctttcacccg aggaatattc atttgggtca tacaacggat cgggcaagag tggcagccgc
    19441 tcatttttac tgtgtatttc tctgccgccg gtcctaagtc aaaaacggat ttataggtac
    19501 gaaagccgac cgtattctga ttgcctggcg gaatttcacc cgtttcaatc ggaccacctt
    19561 cagccgcgcc ccaaaacagc cctttacgtg ctgtccagcg tgacttggga tctgaatact
    19621 ctgcccacga ttgtggcgta cttggcacga catggttgtt attcgcacca ataagttcag
    19681 tcatcatgta atcattgaga cgcttcatct cctgacgatc ggcaatgttg acccctttcg
    19741 tcccttcaac cgcaatcgct ttcaggtttt tcgaacccat tattgcgcca gttcccgcac
    19801 cgccgctgtg gttacggcta ttcaacatgc cagagagagg aataaggttt tccccagcct
    19861 gaccaatagc cgccacacag gtttccgggc tggtcaatcg acaaatttct tccgtcgttg
    19921 cgcgcgtccc ttttccccat aagaaatcgg ctttttccag gctaactttg tcatctttaa
    19981 tcttcagcca taccggtgat ttcgccttcc cttcgataat aatgacgtcg tatccagcga
    20041 atttcatttg cgctgcaaaa aagccaccca tatgggcatc gcgactaaat ttcctttggt
    20101 aaaagtagaa agtgaggtga tatttacgcg agaactacag ggggcaccag atccaggtaa
    20161 tgggccggta gcaaagacta atttattcgc ttcatcgaaa ggtttcgtgc ctagcggtac
    20221 ttcgtcatac ataattttgt agccgaagcc catgccaccg acaaaacttt taaacttact
    20281 ggaatcttcg agggtaatat ttcctgtcgt gagattgact cttaatatat tacctgtcca
    20341 accgttagcc atgatttttt cctttgcaag attcacacag taatatcttt ccactcgata
    20401 atttttaacg cccctgttgg gcaggcgttt gcgcattcac cgcataacac acatttcgag
    20461 gattttttac tttcggtatt tacggtggcc atcatccacg ggcatgccgt agtacaggcg
    20521 ctacagccaa tacaacgttt atgatcgacg gtaatacagc cttctttctg ctgccaggta
    20581 atcgcaccaa tcgggcagac gttcatgcat tgcggctctt tgcattgacg gcaggtgtcc
    20641 gcggtatagt tgagatcgcc atacaggccg ccgccagagc caaccccgtt gtcgccaaag
    20701 aaataattgc gatggatttt gatacgggag aagaatgtcc ctactgagcc atcgttgaag
    20761 ttggtacaag agatttcaca gcggtggcaa cccgtgcatc gcgctcgctg ggtcaccagc
    20821 acccctttgg gtgtatttat caaccctact gtgccgctat caatatcttc ctgtttgcaa
    20881 cccaaaagtg acagtaacgc aggggcaatt gttagaccag caaggccttt tcctgatata
    20941 cgcaagaatt ctaatcgcgt taagccaata tctaatagtg gacgatcaac cgggttcatt
    21001 tatttcgcat cctcttcgca aacacagtgc tgacaattca gcagcctgat attaagcatt
    21061 caactaatac gctgctgact ctgtccccaa caccagatat tttccctaac ccgatagtgt
    21121 atgagaaaag atttaacgcc gataaactaa tcctttatgg ttaggagaaa aaataatgtg
    21181 attattctga gcccttaaca ttgatcgtta tcaattaaaa ttacaaagca agaaattata
    21241 agcgaactga tatttattat catttgaaat aaatttaact taagcgacaa tcatttgtga
    21301 cgttcattgc agaaaataaa aataacatta tcgctataaa ttaaaataca taccgaaatt
    21361 cgtataattt cagcagttgt tatttagaat gattaactgg accgcaactg aagcaagaat
    21421 atcaggatga agcttaccgc ctcatcctga atggtcattt taccagtgcg ttaatgacgg
    21481 tttccatcgc ctgcttttcc agctcactgc gctgaatgc
//
LOCUS       DAJTQQ010000063        21501 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000063 DAJTQQ010000000
VERSION     DAJTQQ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.063"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(20..1018)
                     /gene="fbp"
                     /locus_tag="OO309_002616"
     CDS             complement(20..1018)
                     /gene="fbp"
                     /locus_tag="OO309_002616"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR5847716.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1194..2567
                     /gene="mpl"
                     /locus_tag="OO309_002617"
     CDS             1194..2567
                     /gene="mpl"
                     /locus_tag="OO309_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR5847717.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2650..3201)
                     /locus_tag="OO309_002618"
     CDS             complement(2650..3201)
                     /locus_tag="OO309_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR5847718.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3295..4647
                     /gene="pmbA"
                     /locus_tag="OO309_002619"
     CDS             3295..4647
                     /gene="pmbA"
                     /locus_tag="OO309_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR5847719.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4830..5212
                     /gene="cybC"
                     /locus_tag="OO309_002620"
                     /pseudo
     CDS             4830..5212
                     /gene="cybC"
                     /locus_tag="OO309_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5257..5721)
                     /gene="nrdG"
                     /locus_tag="OO309_002621"
     CDS             complement(5257..5721)
                     /gene="nrdG"
                     /locus_tag="OO309_002621"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR5847720.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5879..8017)
                     /gene="nrdD"
                     /locus_tag="OO309_002622"
     CDS             complement(5879..8017)
                     /gene="nrdD"
                     /locus_tag="OO309_002622"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR5847721.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8411..10066)
                     /gene="treC"
                     /locus_tag="OO309_002623"
     CDS             complement(8411..10066)
                     /gene="treC"
                     /locus_tag="OO309_002623"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR5847722.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10116..11537)
                     /gene="treB"
                     /locus_tag="OO309_002624"
     CDS             complement(10116..11537)
                     /gene="treB"
                     /locus_tag="OO309_002624"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR5847723.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11656..12603)
                     /gene="treR"
                     /locus_tag="OO309_002625"
     CDS             complement(11656..12603)
                     /gene="treR"
                     /locus_tag="OO309_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR5847724.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12788..12841
                     /gene="mgtL"
                     /locus_tag="OO309_002626"
     CDS             12788..12841
                     /gene="mgtL"
                     /locus_tag="OO309_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR5847725.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12982..15678
                     /gene="mgtA"
                     /locus_tag="OO309_002627"
     CDS             12982..15678
                     /gene="mgtA"
                     /locus_tag="OO309_002627"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR5847726.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15884..16270)
                     /gene="ridA"
                     /locus_tag="OO309_002628"
     CDS             complement(15884..16270)
                     /gene="ridA"
                     /locus_tag="OO309_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR5847727.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16343..16804)
                     /gene="pyrI"
                     /locus_tag="OO309_002629"
     CDS             complement(16343..16804)
                     /gene="pyrI"
                     /locus_tag="OO309_002629"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR5847728.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16817..17752)
                     /gene="pyrB"
                     /locus_tag="OO309_002630"
     CDS             complement(16817..17752)
                     /gene="pyrB"
                     /locus_tag="OO309_002630"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR5847729.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17756..17890)
                     /gene="pyrL"
                     /locus_tag="OO309_002631"
     CDS             complement(17756..17890)
                     /gene="pyrL"
                     /locus_tag="OO309_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR5847730.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18171..18566)
                     /locus_tag="OO309_002632"
     CDS             complement(18171..18566)
                     /locus_tag="OO309_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5847731.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18698..19411)
                     /gene="bdcA"
                     /locus_tag="OO309_002633"
     CDS             complement(18698..19411)
                     /gene="bdcA"
                     /locus_tag="OO309_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5847732.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19482..20075
                     /locus_tag="OO309_002634"
     CDS             19482..20075
                     /locus_tag="OO309_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5847733.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20220..20672
                     /gene="tabA"
                     /locus_tag="OO309_002635"
     CDS             20220..20672
                     /gene="tabA"
                     /locus_tag="OO309_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR5847734.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20795..>21501
                     /locus_tag="OO309_002636"
     CDS             20795..>21501
                     /locus_tag="OO309_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5847735.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLL"
BASE COUNT         5189 a         5323 c         5666 g         5323 t
ORIGIN      
        1 tgtggagagg cgcaggagat tacgcgtccg ggaactcacg gataaagcgt tcgacatctt
       61 caaccatatg gtcgttgccg acaaagaacg aacggcgctg gtgcagagtt tccgggatga
      121 tatccagaat acgctctttg ccatcgctcg ctttaccgcc cgcttgttcc gccaggaatg
      181 ccatcgggtt gcactcataa agcaaacgca gtttgccgtc cgggtggctg gcggtgcttg
      241 ggtagagata aataccgcct ttcagcaggt tacggtggaa atccgcaacc agtgaaccga
      301 tataacgcga ggtatacggg cgattggtgg atttatcttc ttcctggcag aatttaatgt
      361 acttcttcac cccgttcgga aacttaatgt agtttccttc gttgatggag taggttttgc
      421 ctttctccgg gaagcgcatc cgttcctggc acaggcagaa aacgcccagc gaaggatcgt
      481 aagtaaaggc gtgaacaccg catccggtgg tgtaaaccag catggtagag gagccgtata
      541 ccacgtaacc tgccgcaacc tgtttgttac caggctggag gaaatcttct tccgttaccg
      601 gcgtgccaac aggcgtaacg cggcggtaga tggagaaaat ggtaccgaca gagacgttaa
      661 catcgatgtt ggacgagcca tccagggggt ccatcagcac cacgtatttt gcgtgttcac
      721 agccttcaaa gacgacaatc tcatcttctt cttcagaggc aatccccgca acgatatcgc
      781 gtgctttcag tgcggctttc agtttttcat tagcgaacaa gtcgagtttt tgctgaacct
      841 cgccctgcac gttctcagca ccgctggcac ccaggatatc aaccagtcct gctttgttga
      901 tatcgcgatg gataatcttg gcgcccagtt ttattgccga cagcaaagca gtgagctcac
      961 cggtagcatg agaaaactcg tgctgctttt cgacaataaa ttcacctaac gttttcataa
     1021 aactttccct gcaatgttta tggagtaaag cgaccgcaac aatcttaaca aataatctca
     1081 atgttgcgct caggtgaatc gcgccagcaa attgcggatt atcctgaaat gcgtttctca
     1141 cttgcccgac atatgcgtaa aatgagcggc agattaaaaa aggatagtga cgtatgcgca
     1201 ttcatatttt aggaatttgt ggcacattta tgggcggtct ggcgatgctg gcgcgccagt
     1261 taggccatga agtaacgggt tcggacgcca atgtgtatcc gccgatgagc accttacttg
     1321 agaagcaagg cattgagctg attcagggtt acgatgccag ccagctcgag ccgcagccgg
     1381 atctggtgat tattggcaac gccatgaccc gtggaaatcc gtgtgtggaa gcggtactgg
     1441 aaaaaaacat cccttatatg tcaggtccac agtggctgca cgattttgtg ctgcgcgacc
     1501 gctgggtgct ggccgttgcc ggtacacacg gcaaaaccac caccgcggga atggcgacct
     1561 ggattctgga acagtgtggt tacaaaccgg gatttgtgat cggcggtgtg ccggggaact
     1621 ttgaggtttc ggcgcgtctg ggcgaaagcg acttctttgt tatcgaagcg gatgagtacg
     1681 actgcgcctt cttcgacaaa cgctctaaat ttgtccatta ctgcccgcgt acgctgatcc
     1741 tcaacaacct tgagttcgat cacgccgata tctttgacga cctgaaagcg atccagaaac
     1801 agttccacca tctggtgcgt atcgttccgg ggcagggccg tattatctgg ccggaaaatg
     1861 acatcaacct gaaacagacc atggcgatgg gctgctggag cgagcaggag ctggtgggtg
     1921 agcagggtca ctggcaggcg aaaaagctga ccaccgatgc ttccgaatgg gaagttttgc
     1981 tggatggcga aaaagtgggc gaagtgaaat ggtcgctggt aggcgaacat aatatgcaca
     2041 atggcctgat ggcgattgca gcggctcgcc atgttggtgt agcgccggca gatgccgcta
     2101 acgcgctggg ttcgtttatt aatgctcgtc gccgtctgga gttgcgtggt gaagcgaatg
     2161 gcgtcacggt atatgacgat tttgcccatc acccgacggc gattctggca acgctggcgg
     2221 cgctgcgtgg caaagttggt ggtacggcgc gcattattgc tgtgctggaa ccgcgctcga
     2281 ataccatgaa aatggggatc tgcaaagacg atctggcacc ttcattaggt cgtgctgatg
     2341 aagtcttcct gctgcaaccg gcgcatattc cgtggcaggt ggcagaagtg gcagaagcct
     2401 gcgttcagcc tgcacactgg agtggcgatg tggatacgct ggcagatatg gtggtgaaaa
     2461 ccgctcagcc tggcgaccat attctggtga tgagcaacgg cggttttggt gggatccatc
     2521 agaaactgct ggatggtctg gcgaagaagg cggaagctgc gcagtaattc ggcttcagcc
     2581 tgatatagca ttgccggata aggcgtttac gccgcatccg gcaaaccata ctcacatcaa
     2641 caacgaaaat tacccttcat tctccgccag ttcgcgcaga tactggaaaa tctggcgtgc
     2701 ggatttcggc ggcttattcc cttctttctc tttcttcgcg ttgcggatca gtgtacgcag
     2761 ttgctgacga tcggcatcgg gccacagatt caatacttca gcgatggcgt catcaccttg
     2821 atcgatcaga cgatcgcgca ggttttccag tttatggaac agcaccacct gctggttgtg
     2881 acggtttttc agcttatcca gcgcctgacg aataggctct acgtcgcgct ggcgcagcat
     2941 cttaccgatg agctgcaact ggcggcggcg accttccatc ttaatacgct gggcgagctc
     3001 aatagccggg cgcagatccg catctaacgg gatcttatcc agcgcgtttt tccccagatc
     3061 aacaatttcc gcgccaaggc gttttagctc ctcggcatca cgtttaattt cacttttact
     3121 gacccagata atttcatcgt cttcgtcttc gatgtcatca ccgggaacgt cgtcgagcca
     3181 gtcttcgggc tgcttagtca tctcaggctc cttaaaaaaa gaggctaatg ttaccagtta
     3241 agatgcgcac tgaaaaacgg ttctctgtta gacttcagag aaactctcta cattatggca
     3301 cttgcaatga aagtaatctc tcaagttgaa gcgcagcgca agattctgga agaagcagtt
     3361 tccactgcgc tggagttggc ctcaggcaaa tcggacggtg cggaagttgc cgtcagcaag
     3421 accaccggca ttagcgtaag cacgcgttat ggtgaagtgg agaatgttga attcaatagt
     3481 gatggcgcgc tggggatcac tgtttatcac cagaaccgta aaggtagcgc atcgtctacc
     3541 gatttaagcc cgcaggccat tgcccgcacc gtacaggcgg cgctggatat tgcccgttat
     3601 acctcgccag atccctgtgc cggcgtggca gacaaagagc tgctggcctt tgacgcaccg
     3661 aatctcgact tgttccaccc tgcggaagtt tccccggatg aagccattga actggcggcc
     3721 cgcgcagaac aggcggcatt gcaggcggac aaacgcatca ccaataccga aggtggcagc
     3781 tttaacagcc actacggtgt caaagttttt ggcaacagcc acggcatgtt gcagggttac
     3841 tgctcaacgc gtcattcgct ctccagctgt gtaattgccg aagaaaatgg cgatatggag
     3901 cgtgattacg cctacaccat tggccgcgcg atgagcgatc tgcaaacgcc agagtgggtc
     3961 ggggccgact gtgctcgccg tactttatcg cgcctgtcac cgcgtaaact ctccaccatg
     4021 aaagcgccgg tcatttttgc caatgaagtg gcaaccgggc tttttggcca tctggtgggg
     4081 gcgatagcgg gtggctcggt ttaccgtaaa tctacctttc tgctggattc gctgggtaaa
     4141 caaattctgc cggactggct gaccattgaa gagcatccgc atctgctgaa agggctggcg
     4201 tcgacgccat tcgacagcga aggtgtgcgc accgagcgtc gcgatattat taaagatggc
     4261 atcctgactc agtggctgct gaccagctac tcggcgcgga aactggggct gaaaagcacc
     4321 ggacatgcgg gcggtattca caactggcgg attgccggac aagggctaag cttcgaacaa
     4381 atgctcaaag agatgggcac cgggctggtg gtgacggagc tgatgggcca gggcgtgagt
     4441 gccattaccg gtgattattc ccgtggtgca gcgggcttct gggtagagaa tggcgaaatt
     4501 cagtatcccg ttagtgaaat caccatcgca ggtaatttaa aagatatgtg gcgcaatatt
     4561 gtcaccgtcg gtaacgatat tgaaacacgc agtaatatac agtgtggttc tgtgctgttg
     4621 ccggagatga aaatcgccgg gcagtaaaaa actggcgcga cctgccgcgc tataaactaa
     4681 aaaattacac aaaatcattc gcactgcatc gagacggaaa gtgaatgagg gccggggagc
     4741 gtactcgcag tacgtgaccc gggcgaatga gcgtagccgt cgatgaggca gtgtgaagga
     4801 tgaagtgtaa ataaaaaagg aagtgagcaa tgcgtaaaag cctgttagct attcttgcag
     4861 tctcctcgtt ggtattcagt tctgcgtcgt ttgccgctga tcttgaagac aatatggaaa
     4921 ccctcaacga caatttaaaa gtgatcgaaa aagcggataa cgcggcgcaa gtcaaagacg
     4981 cgttaacgaa gatgcgcgcc gcagcgctgg atgcgcaaaa agcaacgccg ccgaagctcg
     5041 aagataaatc accggacagc ccggaaatga aagatttccg ccacggtttc gacattctgg
     5101 tcggtcagat tgacgacgcg ctgaagctgg caaatgaagg taaaaagaag cgcaggctgc
     5161 tgcagagcaa ctgaaaacga cccgcaacgc ctatcaccag aagtatcgtt aattcctcat
     5221 ttccctgttg cctgcactca ggtaacaggg aacttatcat cgcaaatgat gcaccacctg
     5281 attgctgctg ccgcgccaga tcagggaagg gtcttttaaa tcctgcacaa atttgccgtc
     5341 gaccagcacg ttaatcaaat caacaacctg catttgcgca gcgttgagtt cgtcgagttt
     5401 atagcctgtc cacacccaga tgtctttacc cggacactcg gcgcggatgc gttttaccag
     5461 tttcagaata tccggcacgt tttgcggatg cagcggatcg ccgccggaga gggaaatccc
     5521 ctggcgtttg atacgagtgt cattcagatc tttaatgatc ttgtcttcca ttgctttggt
     5581 aaatggctga ccagaattta cccgccatgt gcttttgtta tagcaaccgg ggcattcatg
     5641 aacacacccg gagacaaaca gggtgcagcg agtgccgggg ccgttgacga tgtcgacagg
     5701 atagtactga tgataattca ttatttcgcc tggcttatca gtccgcgtgt aggctgcatc
     5761 cggtaatcaa tgcctgatgc gacgctgtcg cgtcttatca ggcctacagc cgttgccaaa
     5821 tgtaggccgg ataaggcgtt tacgccgcat ccggcatttg cttagcgcag agtgaagatt
     5881 aacctatctg cccattcccc aaatgtttaa cgcggcgctt aacttcttcc tgcttaccag
     5941 cgttaaacgg acgtgcatcc gggctaccta aatatccgca cacgcggcga gttaccgaca
     6001 cacgggaggc gtcatggtta ccacatttcg ggcaagtgaa gcctttgctg gtgcactcga
     6061 actcaccggt aaagccacac tcgtagcact catcaatcgg tgtattggtg ccgtaatacg
     6121 gtacatgctg atagctgtaa tcccagacat cttccagcgc tttcaggttg tgctgaatgt
     6181 ttggatactc gccgtagcaa atgaaaccac cgttcgccag cggcgggtaa ggcgcttcaa
     6241 agtcgatctt gtcgtacggg ttcaccttct tctccacatc aaggtggaaa ctgttggtgt
     6301 agtaaccttt atcggtcacg cccggcacca cgccaaactc agcagtatcg agacggcaga
     6361 agcggtcgca caggttttca ctcggcgtgc tgtaaagact gaaaccataa cccgtttctt
     6421 ctttccactg atccactgcc tgacgcagac gttcaacaat ggcgatacct ttcgcgcgaa
     6481 gcttctcgtt gtcgtaaaca tgctcgccgc tgaacagcgc gttaatggtt tcgtggatgc
     6541 cgatgtaacc cagcgaaata gacgcacgac cgtttttgaa gatttcagaa acatcatcgt
     6601 cagcattcag acgtacgcca caagcacctt ccatatagag gatcggggcg acgcgcgctt
     6661 tcacgccttc gagacgagcg atacgggtca tcagtgcctt acgtgccagc accagacgtt
     6721 catccagcag cttccagaag gtggcttcat cgccttttgc ttccagagca atacgcggca
     6781 ggttcaggct gatcacgccg aggttgttac gaccatcgtg gatctgctcg ccgttttcat
     6841 tttcccacac gccgaggaag ctgcggcagc ccatcggggt tttaaacgaa ccggtgacgt
     6901 tcactacctg atcgtagttc aggatatccg gatacatgcg cttgcttgcg cactccagcg
     6961 ccagctgttt gatgtcgtag ttcggatcgc cttttttatg gttcaggcca tcgcgaatcg
     7021 caaacaccag tttcgggaac accgcagttt tacggttttt accgaggcct gcgatacggt
     7081 tacgcaggat cgattcctga atcaggcgcg attcccagct ggtgcccaga ccaaaaccaa
     7141 aggttacaaa cggcgtctga ccgttggcgg tgtgcagggt gtttacttcg tactccagcg
     7201 actggaaggc atcgtagcac tctttgatgg tacgagagtt agcgtagcct tcggcgtccg
     7261 ggatgttcca ctcttctgcg gtcttgcgat gtttgttgta gctggcggta acaaacggcg
     7321 ccagcacttc atcgatacgg ttaatggtgg tgccgccata aatatggctg gcgacctgag
     7381 caataatctg cgcagttacc gcggttgccg tagagatcga cttcggcggt tcaatctcgg
     7441 cgttccccat tttaaaaccc tgggtcagca tgcctttcag gtcgatcaac atgcagttga
     7501 acatcgggaa gaacggcgag taatcgagat cgtgatagtg aatatcgcca cgctcatgtg
     7561 cctgcaccac gtcacgcggc agcaggtgct ggcgtgcata gtgtttagcc acgatcccgg
     7621 ccagcaggtc gcgctgggtt ggaatcacct tgctgtcttt gttggcgttt tcgttgagta
     7681 acgaggcgtt ggtctgctcg accagaccac ggatctcctg gttcaggcga ccgcgttttt
     7741 cacgttcaat gtcgcgatcg tgacggtact cgatgtaagc acgagccagt tgtttgtatg
     7801 gacccgacat cagctggttt tcaactgcgg tctggatctc gttgatatcc acctggttgc
     7861 gcccctgcat ctgctcgttg acaaccgcgg caacagtggc gcaataatcg gcatcatcga
     7921 cttccgctgc tttagctgca cgcagaatcg cttctttgat gcgctctgat ttaaacggca
     7981 ctttgcagcc gtctcgtttc atcacatgcg gtgtcatgat cgctccatat ttttaagaac
     8041 aggttatcca cagaaattgg gaaaggcgtt ttccggtttt ttcgtttcgt tttccggtgc
     8101 tttccgcaat cgccatccgc ttatccacaa cgccgcacct ttttcgtggg gagctgtagt
     8161 agcaattata gtcgattaat acaacatatt gggttgggac gcattttaaa gtctatatat
     8221 agtgctttgc atcaaggatg ttttaacttt ttttgatgta gctcaaagta aaaagcagag
     8281 cgtacggatg acgggcgcta cagcgttatg taaatttttt aatgaatttg ctggttgaaa
     8341 aatcaacaaa aacaacatgc tgacagacaa aaccccggaa tgaccggcat tatcggggct
     8401 tagggaagat ttacttctgt aaccaccaga cagcctcaaa aggccgtaaa ttcatggcac
     8461 agggttgtgg tgaggcttct tcgtagttat gcatcacaag ctgccagttg ccgcgcattt
     8521 gccctggctg ccagggttgg atctcacggc taaggttggc aatgaccagc aaggtttgcc
     8581 ccttccattc acggcgatag caccacaata cagggctgtt tggcagcaga tcctggtaat
     8641 tgccccatgt caggatggct tcttgcttac gcagtgcgat taacttttgg taggtgtaaa
     8701 acacagagga gtcatcggcc agcgcggctt ctacgttgat ttgttgatag ttatcgccta
     8761 ggccaatcca cggttcgcca gccgtaaacc cggcattatc gccgttgctc cattgcatgg
     8821 gcgtgcggct gttgtcacgg gatttactgg cgaggattgc caataactcg tcggcatcac
     8881 gaccatcgtt gcgcagctcg gcaaacatat tgaggctctc cacgtcgcga tagtcagtaa
     8941 tgcgcgtgaa atgcgggttg gtcatgccaa tctcttcgcc ctggtagata tacggcgttc
     9001 cctgcatgcc atgcagcacc atcgccagca tttttgccgc aggcacgcgg tattcacctt
     9061 catcaccaaa gcgagaaaca atgcgcggct gatcgtggtt acaccagaac aaggcattcc
     9121 atgccacgtt gtgcattcct tgttgccagt ggcggaacaa tgttttcaac gccacaaagt
     9181 caggtttagc cagcgtccat ttttcaccac cgggataatc gaccttcagg tgatgaaaat
     9241 taaaggtcat cgacaattca ctgcctgtca gtgccgcgta tcgctggcaa tgctcaaggc
     9301 tggtggagga catttcacct acggtcatta acccgcgtgg cgtaaacaca tcgcggttca
     9361 tctcgtgcaa aaactcgtgt gctcgtggcc cgtcggtgta gaagcgacgc ccgtcgccat
     9421 ccaggtcttc agggaaacgc gggtctttgg agatcagatt caccacatcc aggcgcaacc
     9481 cgtcgacccc acgatcggcc cagaattcac agactttttt cagctctgcg cgtaccgctg
     9541 gattctccca gttgagatcc gcctgttctg gtgcaaagag atgcaaatag tactgttcgc
     9601 tttccgcatg ccagcgccac gcactaccgc caaattttga acgccagttg ttcggtggcg
     9661 tttctggttc gccatcgcgc cagatataaa actggcggta agggctttct ttgttcagcg
     9721 cctcgcgaaa ccaggcatgt tgggtagagg tatggttaaa caccatatcg agaatgatac
     9781 gaatcccgcg cgattttgcc tgcgtcacca gttcgtcaaa atcgtccagc gtgccgtagg
     9841 tgggatcaat cgccgtatag ttcgctacat cgtaaccgtt atcgacctgg ggagagacat
     9901 aaaagggggt tagccagatg gcatcaacgc ccagtttatg cagatagtcc aggcgttgga
     9961 taacgccacg taaatcgccg gtgccgctac ccgtggtgtc ctgaaaactc tttggataaa
    10021 tctggtagat aacgccgttt tgccaccagt ggggaagatt agtcattacg ttattcctgc
    10081 aaatgcgagg gggcgcaatt gcgccccgaa gaaaattaaa caatgtccag cgtgcccagg
    10141 cggtatttcc gctgatagat aaacgaggtg agtacaatcg ggatgatgat ggcgatagcc
    10201 attgccagcg caaatacctg ccagtagctc ggttgaatag agagaattcc cggcaggccc
    10261 cctacgccga taccattcgc cataacgccg ttcaggccgc atagcaatcc tgccagacca
    10321 gaaccaatca tcgcgcacag catcgggaag cgatatttca ggttgatgcc gtacattgca
    10381 ggctcagtga ccccaagcca ggcggagata gcggcaggca cggagatctc gcgttcattg
    10441 tgcttgcggc tggaaatgat aatgcctatc acggcggagc cctgagcgat attcgacagc
    10501 gcaatcagcg gccacactgg cgtgccaccc atgctttgaa tcatctgcaa atcaatagca
    10561 agggtggtct ggtgtacgcc ggtgatcacc agcggcgcgt acaggaagcc aaacaatgcc
    10621 gcaccaatcg gagcaaagct gccggtcatc aggtgacgta ccgcaaaggc aacgccatcg
    10681 ccaatcatgc gaccaaacgg accaatcagc gcatgggcga ggaacaccgc gaggatcagc
    10741 gaacagacgg gcaccaccac cagatagagg taatccggta cgatgcgttt aaggcgagtt
    10801 tcaataacgc ccagcgccag cccggctaac agtgccggga tcacctgcgc ctgatagccc
    10861 actttggcga tgctgaacat gccaaagtcc cacacttccg gcagctgctg cccgagcaga
    10921 taagcgttca tcagctgggg ggaaaccagt gtcacgccaa gcacgatacc aaggatcggc
    10981 gtgccgccca tttttttcac cgctgaccag caaatcccga ccggcaggta gaagaaaatc
    11041 gcttcaccga tcaaccacag aaaatcgtag atcgtttgca gggaagggta catttgcgcc
    11101 agcgtctgac cgttgctcat gggcaaatcg ccgatcacat tgcgaaaacc gaggatcaaa
    11161 ccgccgctaa tcaacgcggg cagcaacggg aagaagatct ccgcgaaatg agagatcaac
    11221 tgctcatgcc atttcatatt ttgccgggcg gcttttttta cctgctcttt atcaacctgc
    11281 gcctgtccgg ttgacgctat tagtgcttga tagtaatcac ccacgttggt gccaatcacc
    11341 acctgaaatt gcccggcatt ggtgaaacag cctttcacca tggggagttg ctcaatttct
    11401 ttcggtctgg cattggccgg ttggttgagg acaaagcgca ggcgagtaat acagtggctc
    11461 accgtcgcaa tattgccgcg cccgccgacc agttcaatca accgatcgat atccgtttgg
    11521 tttattttgc tcatcataaa gccccatggc agatgacatt tttggttggc tgcagaatat
    11581 attgcgcgga aaaatttaaa aacgggaacg ttcccgaaac gcagcgaaga tcacaattta
    11641 tcgttcagga aacgatcagg acagggtggc ggggatgatg atttgttgcg gttcgctgcg
    11701 cccggttacc tgtgcgatca attggcaggc cgcctggcgt ccagcttcgg cgtagccagg
    11761 atctacggtg acgatctccg gatgaaggaa tttcattaac ggcgtattac ctacgctcgc
    11821 cagttgcaag gtgtcgatgc gttgctcttg caggtattta cttgcgccaa gagccagcgt
    11881 gtcggttgcg cacagtaacg cggtagtttc aggcgtaatc acttttgcta cgttttcata
    11941 gccttgcttc atagcaagcc ctggcagagc ggcaacggga tgcagtttat gcgctttgca
    12001 gaacgccagg taggcttcgt gacgtcgctt cccggtcgtc acgtcactat gcggcacgcc
    12061 gagataactg atattgcgat gcccctggtc atagagccgt tgcatcagaa tcttgattgc
    12121 cccttcgtcg tcataacaga ccgaagcaaa gccttttgcg tcacgcgcca gcagaaccag
    12181 cgatgactgc cagtgggcta acatttcttc ggttatgccg gtaaaaccga acagcactac
    12241 gccgtcgata ttacgccgtt tcagcacccc caaatgttcg gcaactaatt gtggggaaaa
    12301 ctgattttcc atcatgattg gatcgtaacc ttgttcatag aacgctggca gcatggtttg
    12361 aacggcgaga ttttctgaca acgaatccag acgggtaaca atgatggcga ccactttatc
    12421 gctttgccca cgcatagcgc gcgcagagcg ggaaggggaa aatccatgct gattcatcac
    12481 tgcttcaaca cgctcgcgcg tgcgctgact cacgccgctt tcgttattca gcacccggga
    12541 aaccgtagat ttccccacgc cgcttaagcg cgcgatgtct ttgatggtca gccgattttg
    12601 catcctgttg tcccataacg tgttgtttaa ttatttgagc ctaactttac ccgtgcattc
    12661 agcaatgggt aaagtctggt ttatcgttgg tttagttgcc agcaggtatt atatcgccat
    12721 aaatgctacg aatattattg gattctcctt attatttgcg gcgctttttt cacttaccgg
    12781 aggttatatg gaacctgatc ccacgcctct ccctcgacgg agattaaaac ttttccggta
    12841 agcccgtctt ttcacggcgt taccggatgc gtaaggccgt gacgttttaa tgtccctgct
    12901 cagctttatt accttcaggt aaggcttcgc cacgcctgaa gacatttctg tactgtttca
    12961 gacagtgcgg agggactcct tatgtttaaa gaaattttta cccggttcat tcgccattta
    13021 ccttcccgtc tggttcatcg cgatccattg cctggcgcgc agcagacagt gaatacggcg
    13081 gtcccgccgt ccttaagtgc gcattgcctg aaaatggcgg tgatgcccga agaagaattg
    13141 tggaaaacgt tcgacaccca tccggaagga ttaaatcagg cggaagtgga atctgcccgc
    13201 gaacaacatg gtgataataa attacccgcc caacaaccgt cgccgtggtg ggtacattta
    13261 tgggtctgct atcgcaaccc ctttaatatt ttgctcacca ttcttggtgc tatttcttac
    13321 gccacggaag atttatttgc cgcaggtgtt atcgcgctaa tggtcgctat ttctacgttg
    13381 ctgaacttta ttcaggaagc tcgttccacc aaagcggcag atgccctgaa agcgatggtc
    13441 agtaataccg cgacggtact gcgagtaatt aacgacaaag gcgaaaatgg ctggctggag
    13501 atcccgatag accagctggt gcccggcgat attataaaac tggcggcggg ggatatgatc
    13561 ccggcagatt tacgtatctt gcaggcgcgg gatctgttcg tcgctcaggc gtcgttaacc
    13621 ggtgagtctc tgcccgtgga aaaagccgct actactcgcc agccggagca cagcaatccg
    13681 ctggagtgcg acaccctgtg ttttatgggc accaccgtgg tgagcggcac ggcacaagca
    13741 atggtgattg ctacaggagc caacacctgg tttggtcaac tggcggggcg tgttagtgag
    13801 caggaaagcg agccgaatgc ctttcagcaa gggatcagcc gcgttagtat gctgctgatt
    13861 cgctttatgc tggtgatggc tccggtggtg ttgcttatca acggttacac caaaggcgac
    13921 tggtgggaag cggcactgtt tgcgctttcg gtcgcggtag gcttaacgcc ggaaatgttg
    13981 ccgatgattg ttacctcgac gctggcgcgc ggcgcagtaa agctgtcgaa acagaaagtg
    14041 atcgtcaaac atctggatgc cattcagaac tttggcgcaa tggatattct gtgcactgat
    14101 aaaaccggca ccctgacgca ggacaaaatt gtgctggaga atcataccga tatctccggt
    14161 aaaaccagcg aacgcgtgct gcatagcgcg tggttgaaca gtcattacca gaccggactt
    14221 aaaaatctgc ttgataccgc ggtgctcgaa ggtacggatg aagagtcagc gcgttcgctg
    14281 gccagccgtt ggcaaaaaat tgatgagatt ccgtttgatt tcgagcgtcg tcggatgtcg
    14341 gtggtagtgg cagaaaatac cgaacaccat cagctggttt gcaaaggtgc attgcaggaa
    14401 atcctcaatg tgtgttcgca ggtgcgtcac aatggcgaga ttgtaccgct cgatgacacc
    14461 atgctgcgta agattaagcg ggttactgat acgctgaatc gtcaggggct gcgcgtggtt
    14521 gcggtggcga cgaaatacct gccagcgcgt gaaggagatt accagcgggc ggatgaatcc
    14581 gacctgatcc tcgaaggata tattgctttc cttgatccgc caaaagagac aaccgctccg
    14641 gcactgaagg cattaaaagc gagtggaatt accgtaaaaa tccttactgg cgacagtgag
    14701 ttagttgcag cgaaagtgtg ccatgaagtg gggctggatg cgggagaggt ggtcattggt
    14761 agtgatattg aaacgctatc tgacgatgaa ctggcaaatc tcgcacagcg caccacgctg
    14821 tttgcccgcc tgacgccgat gcataaagaa cgcattgtga ctttactgaa gcgcgaaggg
    14881 catgtggttg gctttatggg cgatggtatt aatgatgcgc ccgccttacg cgctgcggat
    14941 atcggcattt ctgtggacgg cgcggtagat attgcccgtg aagcggctga tatcatcctg
    15001 ctggaaaaaa gcctgatggt gctggaagag ggggttattg agggacgtcg cactttcgcc
    15061 aacatgctga aatacatcaa attgacggcg agttctaact tcggtaatgt gttcagcgtg
    15121 ctggtggcga gtgctttctt gcccttcctg ccgatgttgc cgttacactt gcttattcag
    15181 aacctgctgt acgatgtgtc acaggtggcg atcccgtttg ataacgtcga cgacgagcaa
    15241 attcaaaagc cgcagcgttg gaatccggcg gatctggggc gctttatggt cttcttcgga
    15301 ccgatcagct cgattttcga tattctgacg ttttgcttga tgtggtgggt attccatgcc
    15361 aacacgccgg aaacgcaaac gctgttccag tcgggctggt ttgtggtggg attactgtcg
    15421 caaacgctga ttgtgcatat gatccgtacc cgccgtgtgc cgtttattca gagctgtgca
    15481 tcgtggccgt taatgatcat gaccgtgatc gtgatgattg tcgggatcgc attgccgttt
    15541 tcaccgctgg ccagttatct gcagctgcag gcgctgccgt taagctattt cccgtggctg
    15601 gttgcgattc tggcagggta tatgacgtta acccagttgg tgaaagggtt ctatagccgt
    15661 cgttacggct ggcagtaaag aataaactgg gcatggtggc ccggattcag attgcagaca
    15721 aagtgagctt tgttcatgcc ggatgcggcg taaacgccca atccggccta caaattcaat
    15781 aaattgcagg aatcacgtag gcctgataag cgtagcgcat cgggcgattt tgcttttgtc
    15841 atcagactta atccgggcat gatagcccgg atttccatca agattagcga cgaacagcga
    15901 ttgcttcgat ctcaatcttc acgtctttcg gcagacgggc aacttcaacg caagaacgtg
    15961 ccgggaaagt ggcgttgtgt tcggtgaaga aggcttcgta agtggcgttt acggttgcga
    16021 agtcgttcag atctttcacg aacacagtgg ttttaacgat gtcgcccact ttcaggccag
    16081 cggcttcgac gattgctttt acattatcca gcgactgacg tgcctgtgca gcgacgtctg
    16141 ccggtacttc accagttttc ggatttaccg ggatctgacc ggaggtgatg atcatattgc
    16201 ccagatcaac gccctgtacg taaggaccga tagctgccgg tgcattttcc gtcgcgatag
    16261 ttttgctcat gatttctcct ttattacagc ggtaaaaagt ctggctcatt atagggagcc
    16321 agacttttat taccaaccgc aattaattgg ccagcaccac attatgggaa aactcttttt
    16381 cacagtattt gcatttaagc gcgatgtcat tggcgcgttt tcgcacggca aagctggatg
    16441 aaaccggttc ggcatggctg atacagttgc tgttcgggca gaccagcaca ttgtcgatgc
    16501 gctctggcag acttgggcgc gatttaccca ccacttcata gttgtcgata cggttaaccg
    16561 tggcttgcgg tgcatacaat gccagttggt ctacttgctc ttcactcaaa aaggtatttt
    16621 cgattttgat cagatctttg cggcccatct cgccagaagg caaattcaga ccaatggtga
    16681 tgcgctgatc tgtttcggtc aacttgaaca gactcaacag cttaaaaccg atctgggcgg
    16741 ggatatggtc aattaccgtg ccgcgtttaa tagcttcaac ctgcaattta ttatcgtgtg
    16801 tcatctctat ttccccttac agtaccagat cgcgattcag aaccagtgcc agtaacgcct
    16861 ggcgagcgaa aatcccgttg cctgcttgct ggaagtacca ggcgtgaggc gttttatcca
    16921 catccgtcgc aatctcatca acacgcggca gcggatgcag cactttcata ttggctttgg
    16981 cgttgtggag atcgctggcg cgaagaacaa actgcgcttt cacgttggcg tactcggacg
    17041 ggtccagacg ctctttttgc acgcgggtca tgtacaggat gtctacttcc gccatcactt
    17101 cttcaataga gctgtgcaga ctccaggcga tccctttttc atcgagcata tccagaatgt
    17161 attgcggcat tgccagcgca tccggcgcga taaagtaaaa acggttgccg tcgaacttcg
    17221 ctagcgcctg ggtcagggag tgaacggtgc ggccatattt caggtcacca accattgcga
    17281 cgtggagatt gtccagacgc ccctgggttt cctgaatggt gaataagtcc agcaaggttt
    17341 gcgtcggatg ttggttggag ccatcaccgg cattcagtac cggtacattg ccagaaaatt
    17401 cggtggctag gcgtgccgca ccttcctgcg gatgacgcat cactatcgca tcgacgtaag
    17461 tgctgataac cgaaatggta tcggccagcg tttcgccttt tttacccagt gatgtattgg
    17521 cgctgtcgga gaagcccacc acgctggcac ccaggcggtg catggaagtt tcgaaagaga
    17581 ggcgggtacg ggtagaggct tcgaagaaac agctggcaat gactttgtgc ttcaacagct
    17641 ctggttgcgg gtttgctttc agtttcgccg ctgtcgccag caccagatta aggtcatcgc
    17701 gactaaggtc gtttatggaa atgatatgtt tctgatatag cggattagcc atcttttatc
    17761 tcctgacacc taggcaaaaa aaagcccctc gattgagggg ctgggaatgg gtgatcaacg
    17821 ggaagaaaaa cggcaggcca gcgtcttttt tcagacgcgg taagacaaaa tgtcgaacac
    17881 actgaaccat acatcctccc ggcaaattgt ccggcattat actcatagcc agaagcggtg
    17941 caagcatttg atgcaatatt ttgtcagcgc aaacggttta tttgaattaa aagtcaaaat
    18001 atatgcattt ttattttcta tgattctgga ggggaagtgt cgttcggtat caataattgc
    18061 agacatttcc tgcttttttc tttttcattt tcacgcaatc agaaatgcat tttattccat
    18121 tcatccgact tatagggcgg agggtgaaaa cgaacggcta acactattgc ttatagctca
    18181 gggatacgcg ctatcacttt aatttcaaaa tcaaagcctg ccagccaggt aacacccacc
    18241 gccgtccagt ttggataagg tggggcgcta aatatttcat ttttcaccgt catgatgtct
    18301 tcagattgtt tttccggatc ggtatgaaag ctcgtaacat caatgatatc gtcaaaagtg
    18361 catcccgcag ctgccagggt cgcatgcaaa ttatcaaagg ccagtctgac ttgttgctga
    18421 aaatcgggtt ctggtgttcc gtcctctcga cttcctactt gcccggaaac aaacagcaaa
    18481 tcgccggaac gaatagccgc agaataacga tgctcagcat acagtgaatg tcggccagca
    18541 gggaaaacag cggttctttc taccatttgg ttatcctcaa gatttacgac atgaacagaa
    18601 gatttatatt tatcgggagc cgcttttagg gggacgacgt tagtaaacaa aacccagaca
    18661 tcatggataa tggctgggct taattgagcg tagtcggtta tgcgccaaac gcgccatcaa
    18721 tggtatgcat cgcgccggta acaaaactgg cttctggccc tgctaaccat gcgaccatac
    18781 cagcgacctc ttccggttgc ccatgtcttt tgatggccat aaaactatgc aacatatcgc
    18841 gcattggccc gttggcggga ttaacgtcgg tatcaattgg tcctggctgg acgacgttga
    18901 tggtgatccc tcgcggtcca aaatcacggg ccagcccgcg cgccatgcct tgcagggcag
    18961 atttactggc ggcataagca gccatgcctg caacaggcat acgatcgcca ttcacggagc
    19021 cgatgattaa gatgcgcccg ccttcgggca tctgccgggc ggcttcaaca gaggcatgat
    19081 aaggagcatg aatattgatt ttgaaaaggc gatcaatatc gtcggcattt aattccaggg
    19141 catcgccaaa gacgccaata cctgcattta ccaccaggat atccaatgcg ccgctcttac
    19201 gaacgacatc aataacagcg tctctgtcag cactatctgt gaatactgct gtcgctccag
    19261 tctcttgtgc cagacgttca gcggcatctt tcgaccccgc ataggtgaat cgtacattgg
    19321 ccccatcggt gacgaaacga cgtacgatag cggcaccgat tccacgactg ccaccgagga
    19381 tgagaactgt cttaccggta aaagcgccca taaggactcc ttgatttatt atgtaacatg
    19441 cattacaaaa ctgttttaac tttctgtcaa caggttttgt aatggtcact aaaaaacaat
    19501 ctcgcgttcc aggtcgtccc agacgtttcg ctcctgagca ggcagtctct gcggcaaaag
    19561 tgctttttca ccaaaaaggt ttcgatgcag taagtgtctc tgaagttact gattatcttg
    19621 gtattaaccc cccgagtctc tacgcggctt ttggcagtaa ggctgggtta tttagccgtg
    19681 tactcaatga atatgtcggt acggaagcta ttccgcttgc cgatattctt cgtgatgatc
    19741 gtccggtagg cgagtgcctg gttgaggtat taaaagaagc ggcgcgcaga tatagccaaa
    19801 acggcggctg cgctggctgt atggttcttg aaggtattca tagtcatgat ccacaagcgc
    19861 gtgatattgc cattcaatat tatcacgccg cagaaacgac catttatgac tatatcgcca
    19921 ggcggcatcc acaaagcgca caatgtgtga ctgattttat gagtaccgtg atgtcagggc
    19981 tttctgcgaa ggcacgggag gggcactcca tcgaacaact ctgtgcaaca gctgccctgg
    20041 caggggaagc gataaaaact cttctcaagg agtgatgctg gccttgatcc gaaaggcggg
    20101 aacgcgcctg ccgataagtt gtgataagac aataattcac gcgttaaggc tggcggaatt
    20161 gatcatcttt tcgtataacg atagaaatga aacattgttt taattaagga gtggaaaaga
    20221 tgatcatcgg aaatattcat aatcttcagc cgtggctacc ccaggagtta cgccaggcga
    20281 ttgagcatat caaagcacac gttacggcag aaacgccaaa gggcaagcat gatatcgaag
    20341 gcaatcgtct gttttatctt atctcggaag atatgaccga gccgtacgaa gctcgccgtg
    20401 cggagtacca tgcccgctat cttgacattc agattgtgtt aaaaggtcag gaaggcatga
    20461 ccttcagcac gcaacctgca ggcgcgccgg ataccgactg gttagctgat aaagacatcg
    20521 catttttgcc ggaaggcgtt gatgagaaaa cagttatcct aaatgaaggt gattttgttg
    20581 tgttttatcc gggggaagtg cataaaccgc tgtgcgcagt gggcgcgcca gcccaggttc
    20641 gcaaagcagt agtgaagatg ctgatggcgt gatgactttt cgccgtaaat aactccaggt
    20701 ttacggcgag tttgtgaaaa gagcgttttt tgatattttt ttgtgagtaa aatttgtaat
    20761 gcttagacgt tcttattaac tcaaggagtt cgtcatgggc aaaatatcag atttgaatta
    20821 ttctcaacac attacattag ccgacaattt taaacaaaaa aatgaagcct tagatacctg
    20881 gtatgtggga atgaatgatt ttgcccgaat tgccgggggg cagaatagca gaagcaatat
    20941 tctttctcct agagcatttt tggagttttt ggcaaagata tttaccctgg gttatgtgga
    21001 ttttagcaaa cgctccaacg aagcgggtag aaatatgatg gctcatattg agttctcatc
    21061 ttattctaaa gatactgatg gcaatgaaaa aatgaagttt tacatgaata atcctgaagg
    21121 ggagcgagcg gatttatcta aggtgaagat agaaattaca cttgcctcag cttctactaa
    21181 agggattcgt gaaggacata cagtcattat atttaaacaa tctgatggtt cgactaatcg
    21241 ttatgaaggg aagtcttttg aaagaaaaga tgatagttca ttacacctga ttactaacaa
    21301 ggttctggcg tgttaccaac gcgaagctaa caagaaaata gcgcgtctat taaataatca
    21361 tcagaagtta aataatatac aggagttaaa tgattcgcag gagttaaata attcacagaa
    21421 gttaaataat tcgcaggagt taaataactc gcaggtgagt tgtaaaggtt cagttgattc
    21481 tacgattacg gatttattag a
//
LOCUS       DAJTQQ010000064        21079 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000064 DAJTQQ010000000
VERSION     DAJTQQ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.064"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            1..623
                     /locus_tag="OO309_002637"
                     /pseudo
     CDS             1..623
                     /locus_tag="OO309_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="TPM domain-containing protein"
     gene            complement(683..1981)
                     /gene="eno"
                     /locus_tag="OO309_002638"
     CDS             complement(683..1981)
                     /gene="eno"
                     /locus_tag="OO309_002638"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR5847736.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2069..3706)
                     /gene="pyrG"
                     /locus_tag="OO309_002639"
     CDS             complement(2069..3706)
                     /gene="pyrG"
                     /locus_tag="OO309_002639"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR5847737.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3934..4725)
                     /gene="mazG"
                     /locus_tag="OO309_002640"
     CDS             complement(3934..4725)
                     /gene="mazG"
                     /locus_tag="OO309_002640"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR5847738.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4821..7055)
                     /locus_tag="OO309_002641"
     CDS             complement(4821..7055)
                     /locus_tag="OO309_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR5847739.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7103..8404)
                     /gene="rlmD"
                     /locus_tag="OO309_002642"
     CDS             complement(7103..8404)
                     /gene="rlmD"
                     /locus_tag="OO309_002642"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR5847740.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8461..11217
                     /gene="barA"
                     /locus_tag="OO309_002643"
     CDS             8461..11217
                     /gene="barA"
                     /locus_tag="OO309_002643"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR5847741.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11449..12789)
                     /gene="gudD"
                     /locus_tag="OO309_002644"
     CDS             complement(11449..12789)
                     /gene="gudD"
                     /locus_tag="OO309_002644"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5847742.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12810..14150)
                     /locus_tag="OO309_002645"
     CDS             complement(12810..14150)
                     /locus_tag="OO309_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5847743.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14152..15504)
                     /gene="gudP"
                     /locus_tag="OO309_002646"
     CDS             complement(14152..15504)
                     /gene="gudP"
                     /locus_tag="OO309_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR5847744.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15938..16387)
                     /locus_tag="OO309_002647"
     CDS             complement(15938..16387)
                     /locus_tag="OO309_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR5847745.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16405..17187)
                     /gene="truC"
                     /locus_tag="OO309_002648"
     CDS             complement(16405..17187)
                     /gene="truC"
                     /locus_tag="OO309_002648"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR5847746.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17187..17516)
                     /locus_tag="OO309_002649"
     CDS             complement(17187..17516)
                     /locus_tag="OO309_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR5847747.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18138..18683)
                     /gene="syd"
                     /locus_tag="OO309_002650"
     CDS             complement(18138..18683)
                     /gene="syd"
                     /locus_tag="OO309_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR5847748.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18751..19599
                     /gene="queF"
                     /locus_tag="OO309_002651"
     CDS             18751..19599
                     /gene="queF"
                     /locus_tag="OO309_002651"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR5847749.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19711..21075
                     /gene="ppnN"
                     /locus_tag="OO309_002652"
                     /gene_synonym="ygdH"
     CDS             19711..21075
                     /gene="ppnN"
                     /locus_tag="OO309_002652"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR5847750.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHHTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5164 a         5629 c         5041 g         5245 t
ORIGIN      
        1 caattgaaca atatgctaca agagtttttg acaattggcg tttaggagat gccaaacgta
       61 atgatgggat actgatcgtt gttgcctagt cggatcgcac tgtccgcatc caggtaggtt
      121 atgggctgga agaaaaggta accgatgctc tggctggaga tatcatccgt agcaacatga
      181 tacccgcatt taaacaacaa aaattagcta agggattaga gttagctata atcgctttga
      241 ataaccaact cacttcacaa caccaatatc cggctaatcc ttcagaaagt gaatcaacgt
      301 cttccactga tcattattac tttgctattt tttgggtatt tgcagtgatg ttcttccctt
      361 tctggttttt tcatcaaggc agtaattttt gtcgcgcatg taaaagtgga gtctgtattt
      421 cagcaatcca tcttttagat ttattcctgt tctccgataa aactttttcc attgctgtat
      481 tttccttctt ttttactttc accatattta tggtctttac ctgtttatgt gtacttcaga
      541 aaagagcatc tggtagaagt tatcattcag acaacagcgg ttccgctgga ggctcaagtg
      601 gcggcgcatc cggccgctgg taactccacc ataaaaaatg ccagcccgaa ggctggcatt
      661 atcaaatcag ataaagtcag acttatgcct ggcctttgat ctctttacga ccgttgtacg
      721 gtgctttttc gcccagagct tcttcgatac gaatcagctg gttgtattta gcaacacggt
      781 cagaacggct catagaacca gttttgatct ggcctgcagc agtaccaaca gccagatcag
      841 cgatggtagc atcttcagtt tcaccagaac ggtgagagat aactgcagtg tagccagcat
      901 ctttcgccat cttgattgca gccagagttt cggtcagaga accgatctgg ttgaatttga
      961 tcaggatgga gttagcgata cctttttcga taccttcttt caggatcttg gtgttggtta
     1021 cgaacaggtc gtcaccaacc agctggattt tgtcgcccag aactttggtc tggtatgcga
     1081 aaccgtccca gtcagattcg tccagaccgt cttcgataga aacgatcggg tactgtttgg
     1141 tcagttcttc caggaagtga gtgaattctt cagaggtgaa cgctttgttg ccttcgccag
     1201 ccagaacgta tttaccatct ttgtagaatt cagaagctgc gcagtccatc gccagagtga
     1261 tgtctttgcc cagttcataa ccagcagctt taacagcttc agcgataaca gccagagctt
     1321 cagcgttgga acccaggttc ggcgcatagc caccttcgtc accaacagca gtgttcatgc
     1381 ctttcgcttt cagaactttt gccaggtgat ggaaaacttc agaacccatg cggatggctt
     1441 ctttcacagt tttcgcgcca accggctgaa tcatgaattc ctggatatca acgttgttgt
     1501 cagcgtgctc accaccgttg atgatgttca tcatcggaac cggcatggag tatttgcccg
     1561 gagtgccgtt cagttcagcg atgtgctcgt acagcggcat acctttagca gctgcagcag
     1621 ctttggcgtt agccagagat acagccagga ttgcgttcgc gccgaatttg gatttgtttt
     1681 cggtgccgtc caggtcgatc atgatcttgt caatgccagc ctgatcttta gcatctttgc
     1741 caatcagcgc ctgagcgatc gggccgttta ccgcagcaac agctttggtt acgcctttac
     1801 ccaggaaacg ggatttgtcg ccatcgcgca gttccagagc ttcacgggaa ccagtagaag
     1861 cacctgacgg agcagctgcc ataccgacga aaccaccctc cagatgtact tcggcttcaa
     1921 cagtcgggtt accacgggag tcgatgattt cacgaccgat gatttttacg attttggaca
     1981 ttaggttttc ctcaagtcac tagttaaact gaaactccag acaaacaacg cgtacccaag
     2041 gtacgcgttg ccgttctaac ttttttactt acttcgcctg acgtttctgg aactcgctgg
     2101 cggctttcac aaagcctgca aacagcgggt gaccatcacg tggagtagaa gtaaactccg
     2161 gatggaactg gcaagccacg aaccacgggt gattcggaac ttcgatgatc tcgaccaact
     2221 gatcatcccc ggaacggccc gcaacgcgca gacctgcatc ttcaatctgt ttcaacagca
     2281 tgttgttgac ttcgtaacgg tgacgatgac gctcaacaat tgtcggcgca ttgtatagct
     2341 ggcgaaccag gctatcgtca accaactggc actgctgtgc gccgagacgc atggtaccgc
     2401 cgagatcgct cttctcgcta cgaacttcaa cgttgccgtt ttcatcgcgc cactcggtaa
     2461 tcagcgccac aaccgggtac ttacagtctg gcacaaattc tgtagagttg gcgttctcca
     2521 tgttggcaac atggcgagcg taatcaatta acgccacctg catacccagg caaatgccca
     2581 gataaggaat attgttctca cgcgcaaaac gcgcggtcgt aatcatgcct tctacgccac
     2641 gatagccgaa accgccaggt acgaggattg cgtccagacc tttaaggatt tcaacgccgc
     2701 gcgtttcaac atcttgtgaa tcgatcagtt tgatgttgac gctgacacga ttcttcagcc
     2761 caccgtgttt cagtgcttcg atcactgatt tataagcatc cggcagttca atgtacttgc
     2821 cgaccatacc gatggtgact tcacttaccg gattcgcttc ttcgaagata acctgttccc
     2881 attcggacag atttgcttcc gggcagttta agctgaatcg tttacaaata taatcgtcca
     2941 gcccctgaga tttcaacagg cccggaattt tatagatgga atcgacgtct ttcagagaaa
     3001 taaccgcttt ttccggaaca ttacagaaca atgcaatctt cgcacgttcg ttcgccggta
     3061 cagcgcgatc tgaacgacaa atcaggatgt caggctggat accgatggag agcagctctt
     3121 ttacagagtg ctgagtcggt ttggttttga cttcaccaga cgctgccatg tacggcacca
     3181 gcgtcaggtg cataaacaga gtgtgctcac ggccaatttc aacagccatc tggcgaatag
     3241 cttcgaggaa cggcaaggat tcgatatcac ctactgtacc gccgatttct accagtacta
     3301 cgtcatgacc ttcgccacct tccagcacgc gctctttgat tgcgttagtg atgtgcggaa
     3361 taacctgcac ggttgcgccg aggtagtcac cgcggcgttc tttacgcaga acgtcagagt
     3421 agatacgacc cgtggtgaag ttgttgcggc ggctcatttt ggtacgaatg aaacgctcgt
     3481 agtgccccag gtccaagtcg gtttcagcgc cgtcttcagt aacgaacact tccccgtgtt
     3541 ggattgggct catagtacct ggatcgacgt tgatgtaagg atccagtttc atgatggtca
     3601 cattgaggcc acgggcttca agaatggctg cgagggaggc tgcggcaatg cctttaccca
     3661 gagaggatac gaccccgccg gtcacaaaaa tatagttcgt tgtcatgctg aacctgagaa
     3721 gttaggttga aaagacgatg gaataaccaa gacgggaaag cagtataccc gaacatgacc
     3781 tgtgccacaa actttcatta tccctcctct tcgccagcgc actattgaaa tcaggagtga
     3841 gaaaatagcc cctttggggt aaatgttttt gacgcaaatc aagcgcttgt catttaaaaa
     3901 atgacacaaa tggcgcttga ccgcgtaatt cccttagaga tcaatttcct gccgttttac
     3961 ctgttgccag acttcttcca ttgtttcgag gtcaacacct gtcatttcca gtccacgcgc
     4021 ggcaacaata cgctccactt cgcgaaaacg acgctcgaat ttttcgttca ctttttgcaa
     4081 tgcgatttct gctttcgtcc ctaaatggcg agccagatta accgtggcaa acagcaggtc
     4141 ccccatttcc tcctccagtt tagcctggtc gacaacagcc tgccgcgcct cgtacatcac
     4201 ctcgtcgatc tcttcgtaga ctttatcaac caccgggcca agcgtcgtcc agtcgaagcc
     4261 aacgttggcg caacgtttct ggattttttg cgcccgcatt aaagccggta aactacgagg
     4321 aatatcgtcc agcgccgaat gctgcgcttt ctgcgcgcgc tcttcggttt tgatttgctc
     4381 ccaacgggca agcacttcac tactgttttc ggcagaacta tcagcaaaaa catgcggatg
     4441 gcgacgctct aatttatcgc taatagcagc gcaaatatca ttaaagtcaa agcgcccttc
     4501 ttcctgagcc atttgcgcgt aaaacaccac ctggaatagc agatcgccca gttcaccgcg
     4561 aagatcgtca aaatcttcac gggcgatggc gtccagcact tcgtaggttt cttcaagggt
     4621 gtaaggcgca atggtggcaa atgtctgctc tttatcccac gggcagccgt tttccggatc
     4681 gcgcaggcgc tgcataatag tgagcaaacg gtcgatttga ttcattgaat tgtcctgaaa
     4741 attgcgggtc tgtcaggtgt aataccttcg agtaaaagat atcgctggat acggctgacg
     4801 ccatatccag ctatgtaaga ctaactcccg tgcaaccgac gcgcgtcgat aacatccggc
     4861 acctggttga gtttacccag cacgcgcccc agcacttgca ggttgtaaat ctcaatggtc
     4921 atgtcgatgg tcgccagttg ctgtttggtg tcgctacggc tggcaacgcc aagcacgttc
     4981 accttctcgt tggcgagaat ggtcgtgata tcacgtaaca acccactacg atcattagcc
     5041 accacgcgga ccaccagcga atatccggcg gagtagctct caccccatac cgcgtcaaca
     5101 atgcgttctg gcgcatggga gcgcagttcc gccagttgtt cgcaatcggc gcggtgtact
     5161 gaaataccgc gtccctgggt aatgaagccg acaatctcat ctccaggaat cggctggcag
     5221 cagcgcgcga tgtggtgcat caggttacca acaccttcaa ccaccacacg accgttatct
     5281 ttactgcggt tttgcggtgt gtagcttttt tgctgaagct gtttcagcgc ggcggcgtcc
     5341 tgctcttcgg cactcggctt attaaattgc gattgcagga agttcaccat ctgattgaga
     5401 cggatatccc cgccaccaat cgccgccagc aactcgtcga catcattgaa gttgtaacgc
     5461 ggcagcagat gtttttctgc ttctttcagg ctgatcccca gatgttccag ctcgtcgtca
     5521 aggatttgcc gcccagccag aatgtttttg tcacggtcct gtttacggaa ccaggcgtga
     5581 attttcgaac gcccacggct ggttgtgacg taaccgaggt ttgggtttaa ccagtcacgg
     5641 ctggggttcg gctgtttctg ggtgataatt tcaatctggt cgcccatttg cagctggtag
     5701 gtgaacggca caatgcgccc gccaattttt gccccgatgc agcggtgtcc gacatcactg
     5761 tggatgtggt aagcgaagtc cagcggcgtt gatcccgcag gcaaatcaac gacatcacct
     5821 ttcggcgtaa agacatacac ccggtcgtca aacacctgac tacgtacttc gtcgagcatt
     5881 tcgccggaat cagccatctc ttcctgccac gcaatcagtt tacgcagcca ggcaatccgg
     5941 tcttcatgtc ccgaacgtgc gccgccagct gccgcgccct ctttatattt ccagtgcgca
     6001 gcaacaccca actccgcatc ttcatgcatc tgtttggtgc ggatttggat ctcaacggtt
     6061 tttccacccg gccccagaac cacggtatga atagactgat aaccgtttgg tttcgggtta
     6121 gcgacgtaat cgtcaaactc atccggcagg tggcgatagt gagtgtgcac tatccccagt
     6181 gcggcataac aatcctgtaa acgctcggcg acaatacgta ccgcacgcac atcaaacagc
     6241 tcatcgaagg cgaggttctt tttctgcatt ttgcgccaga tgctgtagat gtgtttcgga
     6301 cgaccataca cttcagcttt aacgccttca gctttcatct cagcgcgcag atggccgaca
     6361 aactcttcga tatagtgttc gcggtcgaga cggcgttcat gcagcagttt tgcgatgcgt
     6421 ttgtattcgg tcgggtggag ataacggaag cagtaatctt ccagctccca tttcagttgc
     6481 ccaatcccaa gacggttcgc caacggcgcg tagatattgg tgcactcttt tgccgccagt
     6541 acacgttcat cttccggcgc atcttttact tcgcgcagat gagcaatacg ctccgccagt
     6601 ttgatgacta cgcagcgaaa atcatcgacc atcgccaata acatccggcg aacgttatcg
     6661 acctgttcgg aggaaacaga atcagtgtgc gtcgctttca gctggcggat cgccgccata
     6721 tcacgcacgc cgtgaataag gttaacaacc gacttaccga cgctctcacg cagcacatct
     6781 tcgctgacta cgttggcatc agccagaggg aacagcagcg ccgcccgcag cgtgtcaatg
     6841 tccatactta atgtcgagag gatctccacc atctcaacac cacgccacaa taacagactg
     6901 gcatccggat gcccctgcgt ctgttgcaga caatacgccc aggtttcggc taagcactca
     6961 cacgacttct ggctggtaat acccagactt gcgatccatt tttccggatc aaattcacca
     7021 gccttattga tatgtgcact tcttaccgca accatcgtcc tctcctttag ggaccagacc
     7081 tgccgaaatc ggcaaatcgt aactatttaa cgcgcgagaa aagtaccatc gattccagat
     7141 gtcccgtgtg tgggaacata tccagcatcg ccagtcgcgc aatggtatat cctgctttta
     7201 ataacgcttc gctatcccga gccagcgttg cagggttaca ggatacataa actatacgaa
     7261 taggttccag ttttataatt tgctgcataa cacctgcggc acctgctcgc gccgggtcca
     7321 gcaacacttt atcgaagccg tttttcgccc acggctgctt tgtgacatct tcttcaagat
     7381 tttcgtgata aaacgtcaca ttctgtaagc cattaagacg cgcattctgc tggccttttt
     7441 ccaccagcgc aggaacacct tctacaccga ccacactggc agcttgtgtc gccaatggca
     7501 gtgtaaagtt gcccataccg cagaacagat ccagtacgcg atcttcaggt tgcacatcca
     7561 gccattccaa cgcacgcgct accatttttt ggttcacacc cgcattgacc tgaataaaat
     7621 cgcgcgggct aaaagttaag cgcaacccgt ttgagtcata ccagggcatc tcaccagaga
     7681 cggtttcgag tatctcacta tcgggggcga gatacagatc caggccttca gaatgcgaaa
     7741 agcgttccag tttttcgcga tctgccgaac ttagcggtgc ggtatggcgc aaaatcatca
     7801 gcgtgccgct ggttgcctgt accagttcaa catgaccaag atggcgcata gcttgtaagc
     7861 tgcccagaca tgccctgact ttgggcagca atgcttcaag ttggggcgct aaaatggggc
     7921 attgtttgac gtcgacaatg tcactggagc ccgctttgcg aaaccccatc tgaagttgct
     7981 gtgttttcgg taagtagttc agacttaaac gcgcgcggcg gcgatagccc cagggaacat
     8041 cggcgatcac ttcagagaca tcgtgtttca ttaatcgggc gagtgccgca cttttgcttc
     8101 gctgctgtaa atccacgctg gcgtgttgtt gctgacagcc gccgcatacg ccaaaatgag
     8161 gacagcgtgg cgtttcgcgt tccgggctat cgcttaaccg gcgtacgact ttagcgcggg
     8221 catactgttt tttatcttca gtaacagcaa cttccgcgtt ttcctgcggc aataatccgg
     8281 ggataaataa cgttttgccg ttatgtcgcg ccacgccctg accaaaagag tcgaggtcgt
     8341 tgactgaaac ggttatgatc tgacgcgtcg tcgtgcgtcg ttttgcagag tagaattgcg
     8401 ccattggcga gactttctca atttaacagt gtgaccttaa ttgtcccata acggaactcc
     8461 atgaccaact acagcctgcg cgcacgcatg atgattctga tcctggcacc gaccgtcctt
     8521 attggtttat tgctgagtat ctttttcgtc gtgcatcgct ataacgactt gcagcgtcaa
     8581 ctggaagatg ccggtgctag cattattgag ccgcttgcag tttctactga atatggcatg
     8641 agcctgcaaa atcgcgaatc tatcggtcag ttaataagcg tactgcatcg tcgccattcc
     8701 gatattgttc gcgcgatttc ggtttatgat gaaaataacc gactctttgt cacctccaat
     8761 tttcatcttg atccctcatc aatgcagctc ggcagcaacg tgccgtttcc tcgccagctc
     8821 actgtcactc gtgacggcga tattatgatc ctccgcacgc cgattatttc tgagagttac
     8881 tcccccgacg aatcgcccag tagcgatgcc aaaaatagtc aaaatatgtt gggatatatt
     8941 gcgcttgagc tggatcttaa atcggttcgc ttgcagcaat ataaagagat ctttatttcc
     9001 tgcgtgatga tgctgttttg tatcggtatt gcgcttattt ttggctggcg cttaatgcgc
     9061 gatgtaaccg gtccgattcg caacatggtg aataccgtcg accgcatccg tcgcgggcaa
     9121 ctcgacagcc gggtggaagg atttatgctc ggcgagctgg atatgctgaa aaacggtatc
     9181 aactcgatgg caatgtcgct ggctgcttat cacgaagaga tgcagcacaa tatcgaccag
     9241 gcgacgtccg atctgcgtga aacgctggag cagatggaaa ttcagaacgt tgagttggat
     9301 ctggcgaaaa agcgcgccca ggaagcggcg cgtattaaat ccgagtttct ggcaaatatg
     9361 tcacacgagc tgcgtacacc actgaatggt gttattggct ttacccgcct gacgctgaaa
     9421 acagaattaa caccaacgca gcgcgatcac ctgaatacga ttgaacgttc ggcaaataat
     9481 ttgctggcaa ttattaatga tgttctcgac ttctcgaaac tggaagcagg taagctgatt
     9541 ctggaaagta ttccattccc actacgcagc acgctggatg aagtcgttac tctgctggca
     9601 cattcttctc acgataaagg gttggaactg acgctcaata ttaaaagcga cgtgcctgat
     9661 aacgtgatcg gcgacccact gcgattacag caaatcatca ctaacctggt ggggaatgca
     9721 attaaattca ccgagaatgg caacattgat attctggtag aaaaacgtgc gctgagtaat
     9781 accaaagtgc agattgaagt gcagattcgg gataccggca ttggtattcc tgaacgcgat
     9841 caatcgcgct tattccaggc cttccgacag gctgatgcca gtatttcccg ccgtcatggt
     9901 ggcaccgggc tggggctggt gattacacaa aaactggtta atgaaatggg cggcgatatt
     9961 tcgttccata gccagccgaa tcgcggttca actttctggt tccacattaa tctcgatctg
    10021 aacccgaaca ttattatcga agggccatcc atccagtgcc tcgcaggtaa gcgcctggcc
    10081 tatgtcgaac caaactccgc agcagcgcaa tgcacgctgg atattttaag tgaaacgccg
    10141 ctggaagtgg tttatagccc aacgttctcc gcgctgcctc ccgcgcatta cgacatgatg
    10201 ttgttaggca tcgcggtgac cttccgcgag ccgctaacaa tgcaacatga gcgattagcg
    10261 aaagcggtat cgatgaccga tttcctgatg ctggcacttc cttgccatgc acaagttaat
    10321 gctgaaaaac tcaagcaaga tggtatcggc gcgtgtctgc tgaaaccatt aacacctacg
    10381 cgcctgttgc ctgccctgac ggaattttgt catcacaaac aaaacacgct tttgcctgta
    10441 accgatgaaa gtaagctggc aatgacagtc atggcggttg atgacaaccc cgctaacctg
    10501 aaacttatcg gcgcattgct ggaagatatg gtgcaacatg tggaactttg cgatagcggg
    10561 catcaggcag ttgaacgggc gaaacagatg ccgttcgatt tgatcttaat ggatattcaa
    10621 atgccggaca tggatggcat tcgggcctgc gaactcatcc accaactccc gcatcagcga
    10681 caaacgccgg ttatcgcggt aacggcgcat gcaatggccg ggcaaaaaga gaagctgctt
    10741 ggcgcaggga tgagcgatta tctggcgaaa ccgattgaag aagagcgatt gcataatttg
    10801 ttgttgcgct acaagcctgg cagcggtatt tcgtctcgcg tcgtgacgcc cgaagtcaac
    10861 gaaattgtgg tgaacccgaa tgcgaccctc gactggcaac tggcactacg ccaggcagca
    10921 ggaaaaaccg atttagcgcg cgatatgctg caaatgttac tcgatttcct gcctgaagtt
    10981 cgcaacaaag ttgaggaaca gctggctgga gaaaacccgg aaggcctggt tgatttgatt
    11041 cataaactgc atggcagttg cggctatagc ggcgtaccac gtatgaagaa tctctgccag
    11101 ctgatcgaac aacagctacg tagcggtact aaagaagaag atttggagcc ggagctgctg
    11161 gaactgttgg acgagatgga taatgtcgcg cgcgaagcca gcaaaattct cgggtaacgg
    11221 acgtcttgtc gagtagcaat cggcttttta tgacgctggt ttcagattgg tgacaaagtg
    11281 cctgtgttta tgccggatgc ggcgttaacg ccttatccgg cctacaaaac caattaaatt
    11341 caatgaatta aaaaatgatg tagacctgat acatagcaca acaggctatt ttgcgtttag
    11401 catcagtctc aaaccggctc cagatagagc cggttttggt tttctgtctt aacgcaccat
    11461 gcacgggcgc ttgttatcga acgtccagcc aggaatcaga tactgcattc ccatcgcatc
    11521 gtcacgcgcg ccaagcccgt gtttctgata cagctcatgg gctttcatca cttgatccat
    11581 atcgatttct acacccagcc ccggtttttc tggcacctgt accagcccgc ctttgatctc
    11641 aaacggttct ttggtcaggc gctgattacc ttcctgccag atccagtgcg tatcaatagc
    11701 agtaatttta cccggtgcag cggcggcaac atgggtaaac atcgccaggg aaatatcgaa
    11761 gtggttgttt gagtgtgaac cccaggtcag gccaaattca tggcacattt gcgccacacg
    11821 taccgaacct tgcatagtcc agaaatgcgg atccgccagc gggatatcaa cggattgtaa
    11881 cgagagcgta tggcccatct gccgccagtc ggtggcgatc atattggttg cagtcggcag
    11941 gccagtcgcg cgacggaact ctgccatcac ttcacgcccg gagaaacctt gctccgcacc
    12001 acacggatct tctgcataag ccagcgaacc tttcaggtat ttaccaattt taattgcttc
    12061 gttcagcgac caggcaccgt taggatcgag cgtaatacgc gcctgcggga agcgttgcgc
    12121 cagtgccaca atagactcgg cttcttcttc cccggccagt acaccgcctt tcagtttgaa
    12181 atcgttgaag ccatattttt catacgccgc ttccgccagg cgcaccaccg catccggcgt
    12241 catcgcttct tcatgacgca ggcgatacca gtcgcatgag tcatccggct ggctttgata
    12301 cggcagaggt gtggctttgc gattaccgac gaagaacaga taaccgagca tttcgacttc
    12361 gctacgctgt tgaccatcgc ccagcagcga tgccacgttt acccccagat gctgccccag
    12421 cagatccagc attgccgctt ctatcccggt aactacatga atagtggtac gcaaatcaaa
    12481 tgtctgcaaa ccgcgcccac tagcatcacg atcggcaaaa gtattacgca ccagcgtcag
    12541 aacgtttttg tattcaccca gcgttttacc taccaccagc ggaatcgcat cttccagcgt
    12601 tttacggatt ttctcgccgc cgggaatttc ccctacgcca gtgtgaccgg aattatcttt
    12661 gataatcaca atattacgcg tgaagaacgg tgcgtgtgca ccactcagat tcatcagcat
    12721 actgtcatga cccgccaccg ggatgacctg catttcagta acaacaggcg tcgtaaattg
    12781 agaactcata tttatgtcct ttttcagaat caatgacggc cgaaaacggg acgtttacgg
    12841 tcaaaggtcc agccggggat caggtactgc atcggacctg cgtcgttacg cgcaccgcaa
    12901 ggcagacgtt tataggcctc atgtgccttt tgtacctgtt cccagtccag ttccacgccc
    12961 agaccgggcg catcaggaac ggcaattttt ccgtttttaa tctccagcgg atttttggtc
    13021 aggcgacaat cgccctcctg ccaaatccag tgggtatcga tagcggtagg attacccggt
    13081 gccgccgcgc ccacatgggt aaatatcgcc agagagatat cgaaatggtt gttggaatgg
    13141 cagccccagg tcagccccca gtcgtcgcaa agctgcgcca cgcggactgc accggaaagc
    13201 gtccagaagt gcggatcggc aagtggaata tctaccgcat tgagcatcac cgcatgaccc
    13261 atttcgcgcc agttggtggc gatcatgtta gtcgcgacgg gcaagccggt cgcccgtcga
    13321 aactccgcca tcacttcacg tccggagaag ccctgttctg cgccgcatgg atcttcggca
    13381 taggtaagaa catcattcag ccctttgcat aaagaaatgg cttcatcaag cagccatgca
    13441 ccgttgggat caacggtaat ccgcgcatcc gggaagcttt tcttcaatgc acgaacagtg
    13501 tcgatttctt gctcgccagg taacaccccg cccttaagtt tgaaatcttt aaagccatag
    13561 cgatcctgtg aggcttccgc cagacgcaca acggcttcgc tgttcatcgc tttctgatgg
    13621 cgcaactgat accactcatg gttgcccggc gtattttcca gataaggaag atcggttttg
    13681 gtccgatcac ctatataaaa cagataaccg aggacggtaa tagtctcgcg ttgcttgcct
    13741 ggccccaaca gttcgcagac cggaacattc agcgccttac ctagcaggtc aagcaaggcg
    13801 gcttccagcg ccgccacggc attaacgcgc aattcaaaag tccaggcacc tttgccaaag
    13861 gtatcaaaat cggctgcctg attaccttta tgtacctgct gaaccacttt attcaggcgc
    13921 gcaacttcct ggcccagaac catcggaata gcatcgacca gtgtttgata aatcacctct
    13981 ccgcccggcg cttcaccaat gccggtatgc ccggcgttat cggtgagtac cacgatattg
    14041 cgggtgaagt atgcgttatg cgcaccacca atattgagca acatgctgtc atgtccggcg
    14101 accggaatga ctttcatatc agtaataaca ggactggatt gtgtcgccat cttattgccc
    14161 cgcgacaggt ttcagctcga tacgcttgat atcgcccacc agcaccaggt agctcagtac
    14221 cgcgattagg gcatgaacac caacataaat cagcgcccca ttaaacgagc cagtcgtgcc
    14281 aacgatataa ccaattgcga ttggcgtgac gatgccagaa atgttaccga acatgttgaa
    14341 caggccaccg gaaagaccgc tgatctcttt tggcgcggta tctgccatta ctgcccaacc
    14401 cagcgccccg atgcccttac cgaagaaggc cagcgccata aagccgatga tcatccactc
    14461 aacgttgacg tagttgcaga acaccatcac catcgacagc aacatgccca ttacgatcgg
    14521 tgttttacgc gcaatgttca gcgatcccgt gcggcgcatc agccagtcgg aaataattcc
    14581 acccagcaca ccgccgataa aaccgcaaac cgccggaacg gaagccacaa agcccgcttt
    14641 cagaatcgac atcccacgcg cctgcaccag ataaaccgga aaccaggtaa tgaaaaagta
    14701 agtcagggcg ttgatacagt actgaccgat ataaacgccg atcatcatcc gtgacccgag
    14761 cagctgtttg atctgccccc acttcacgct gaacggcact ttaactttgg tgctttgctg
    14821 atccatattg atcagcgcgc cacccgcggc gatatactcc agctctttct gatttacccc
    14881 cggatgttga tttggctcgt ggatgacttt caaccagata aagctgatga caatgcccag
    14941 accgcccata aagaagaaga cgtgtgacca gcccacttca tgcgtcagcc agcccataat
    15001 cggcgcgaag atcaccgttg cgaagtattg agcggagtta aaaatcgaca ccgccgttcc
    15061 cctttcctgc accggaaacc aggccgcaac aatgcgactg ttgccgggga aagatggcgc
    15121 ttcagcaagc ccgaccagga agcgcagagt aaacagggca acgataatgc cgaatccact
    15181 aaagatatcg acgaagcctt gcagcaaggt aaacatcgac cagataaaga tcgaccagaa
    15241 gtagacgcgt tttgaaccaa aacgatccag caaccaacca ccagggatct gcccgataac
    15301 ataagcccat gagaaggcag agaacacata gcccattccc acgggatcaa ggccgatatc
    15361 tttggccatt tccgaaccgg caatagaaag cgtagcgcgg tcgccgtagt tgaaggatgt
    15421 gacgataaac aacatcacca ctatccagta acgagcattc gtgcgttttt ccacactgct
    15481 cgcagcctga cttaaagaac tcattgttgc actcctgaaa attcgcgtta gccacgctca
    15541 ctctggactg cgacatcgcc aggaaatcag aggtgacgta gggtgttttt tgccgttttt
    15601 ataggtcgtt cgccgaatac ggcgcgtgtt tatatctggc aatagcagta taaaaagcgc
    15661 gccatagcgg ctcaccgtgc aacaacacaa cattaatgcg ttcaatgagg cccgattttg
    15721 gcattagccc tggacggtgg aatccacttc acggaaatga aaacaagaac aagaaaggaa
    15781 gggtaaaaac gaagaaataa agaagagtat gaaatggatc gcttgactct aggcaaacgc
    15841 cagtaaaaat ccgcgctatg aagcagtttt tactggcatt tgcctgaaaa gattcgattc
    15901 agcaccgcta aaacgacatt taccgctcgc tgacatatca ggacaacagc gtgccccact
    15961 gttcgaccca cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca
    16021 gcatttcgcc aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc
    16081 cattgcaaaa atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg
    16141 gctggaagcc cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt
    16201 cgccctgccc ggtcgtggac gtaaccacca gaacatactt atcctgatag ggcagccagt
    16261 cgcttaattc aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt
    16321 ctgcttcttc cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt
    16381 ccgccatacc gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc
    16441 actgaactca accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc
    16501 ctgcatccag gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg
    16561 cgtcaatgac aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc
    16621 gctgcgattc tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg
    16681 ggcaagatgt cgtcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc
    16741 gtagcgcgtg gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg
    16801 ataatgcgtc actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt
    16861 gtccagttct tccaccagtg gataatccaa caccgcttct tccatcaacc agccgcgcac
    16921 aatcgcatgg taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc
    16981 ggcctcgctg gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc
    17041 agtaaaaaca tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc
    17101 gcgatccagc cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca
    17161 ttcatcctga tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt
    17221 ttttccaact cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca
    17281 taatagggtg caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc
    17341 attcgcggga tcagccccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac
    17401 ggttgggtac tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca
    17461 cgcagtaacg cttcaagcgc ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag
    17521 caacctcaat aagaaaaact gccgcgaagg atagcaggaa taaaaaaagg gagcactgta
    17581 ttcacagtgc tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat
    17641 ccttgacaac ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg
    17701 ctcacaccac cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg
    17761 aatcatcctg accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc
    17821 atcctcttcg cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga
    17881 agtgtcatca tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg
    17941 tctttttcgc gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg
    18001 aaagttaaga attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt
    18061 ttaagtattc gcttatttcc tggcgatctc ctacagagtc tgtaagagat ctctcacaac
    18121 cagatggggt atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt
    18181 gaggctaaat tcgtccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga
    18241 ttacacaccg agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggcggc
    18301 agttttagcc ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag
    18361 tcgtcttcac tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata
    18421 tccccggcaa actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa
    18481 gcgcgttcaa ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg
    18541 gcatcttcgg tagttgaaat aatgcacggt gaaggaacac cgtacaattc ctcacttaac
    18601 ggccagcttt tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg
    18661 gcttgtgcgg tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg
    18721 tttagtttat ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt
    18781 gcgggcctga ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg
    18841 caaggcgttc cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct
    18901 tttcacggta cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg
    18961 ccgcaggtcg ctgtcggtca tgttgaactt gattacacca gcgtaaatct gattgagtcg
    19021 aagagtttta agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag
    19081 gtgcgccaga cgctggagcg cgacttaagc acttgcgctc agggtaagat tagcgtggcg
    19141 ttatatcgtc ttgatgaact ggaaggccag ccgataggtc atttcaatgg cacttgcatt
    19201 gatgaccagg atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc
    19261 accagcggtg aaaaagtagt ggaagagacg ctcgtcagcc acctgctgaa atcaaactgc
    19321 ctgatcaccc atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt
    19381 gacagagaaa aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa
    19441 cagtgcgtgg aacgcatctt taatgacctg ttacgcttct gccagccaga gaaattgagc
    19501 gtttacgcac gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc
    19561 gattttgtcc catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg
    19621 ctggattcac gcagaaggtt gtgaaaggtc atcaggcagg gctattgtaa tcaaagggaa
    19681 tgacgatatt cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc
    19741 atggatatgt tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc
    19801 tatcaactgt ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac
    19861 agcaaagaat tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg ccgtgaacgc
    19921 ggcgtaaagc tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt
    19981 cgcgctttgc aggccaactt gttcgcggtt ctgcgagaca ttctcttcgt ttacgggcaa
    20041 atccatcata ccgttcgttt tcccaacctg aatctcgaca actccgtcca catcactaac
    20101 ctggtctttt ccatcttgcg taacgctcgc gcgctgcatg tgggtgaagc gccaaatatg
    20161 gtggtctgct ggggcggtca ctcaattaac gaaaacgagt atttgtatgc ccgtcgcgtc
    20221 gggaaccagc tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc aggagcgatg
    20281 gaagcgccga tgaaaggtgc tgcggtcgga cacgcgcagc agcgttacaa agacagtcgt
    20341 tttattggta tgacagagcc gtcgattatc gccgctgaac cgcctaaccc actggtcaac
    20401 gaattgatca tcatgccgga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac
    20461 ggcatcatta tcttccctgg cggtgtgggt acggcagaag agttgctgta tttgctggga
    20521 attttaatga acccggccaa caaagatcag gttttaccat tgatcctcac cggcccgaaa
    20581 gagagcgccg actacttccg cgtactggac gagtttgtcg tacatacgct gggcgaaaac
    20641 gcgcgccgcc attaccgcat catcattgat gacgccgctg aagtcgctcg tcagatgaaa
    20701 aaatcgatgc cgctggtgaa agaaaatcgc cgtgatacag gcgatgccta cagctttaac
    20761 tggtcaatgc gcattgcgcc agatttgcaa atgccgtttg agccgtctca cgagaatatg
    20821 gctaatctga agctttaccc ggatcaacct gttgaagtgc tggctgccga cctgcgccgt
    20881 gcgttctccg gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc cattgaagag
    20941 tttggtcctt acaaaatcaa cggcgataaa gagattatgc gtcgtatgga cgacctgcta
    21001 cagggttttg ttgcccagca tcgtatgaag ttgccaggct cagcctacat cccttgctac
    21061 gaaatctgca cgtaatctc
//
LOCUS       DAJTQQ010000065        20925 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000065 DAJTQQ010000000
VERSION     DAJTQQ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.065"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..814
                     /locus_tag="OO309_002653"
     CDS             <1..814
                     /locus_tag="OO309_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HCR5847751.1"
                     /translation="DELLRAAEIPRSTFYYHLKALSKPDKYADVKKRIGEIYHENRGR
                     YGYRRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKRYRSYRGEVGQTAPNVLQRD
                     FKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERPVMNMVENMLDQA
                     FKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLDNAVVECFFGTLK
                     SECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQTYMPRV"
     gene            916..1068
                     /gene="hokA"
                     /locus_tag="OO309_002654"
     CDS             916..1068
                     /gene="hokA"
                     /locus_tag="OO309_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR5847752.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(1255..1467)
                     /gene="cspA"
                     /locus_tag="OO309_002655"
     CDS             complement(1255..1467)
                     /gene="cspA"
                     /locus_tag="OO309_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR5847753.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1748..2038)
                     /locus_tag="OO309_002656"
                     /pseudo
     CDS             complement(1748..2038)
                     /locus_tag="OO309_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            2472..3182
                     /locus_tag="OO309_002657"
     CDS             2472..3182
                     /locus_tag="OO309_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR5847754.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(3232..4206)
                     /gene="ghrB"
                     /locus_tag="OO309_002658"
     CDS             complement(3232..4206)
                     /gene="ghrB"
                     /locus_tag="OO309_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR5847755.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(4310..4960)
                     /locus_tag="OO309_002659"
     CDS             complement(4310..4960)
                     /locus_tag="OO309_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR5847756.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            5123..7321
                     /locus_tag="OO309_002660"
     CDS             5123..7321
                     /locus_tag="OO309_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5847757.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALAGGYTGHLGDY
                     STGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGL
                     SYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWH
                     DEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTML
                     MAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGDPTRRSAVLSSM
                     QGSLPGGCDAVDKIPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQD
                     TNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMK
                     QVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVE
                     LPPFAEFWQANQLIEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYG
                     YPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVT
                     IHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGI
                     CKNGAVNVLTKDLPSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(7290..7730)
                     /locus_tag="OO309_002661"
     CDS             complement(7290..7730)
                     /locus_tag="OO309_002661"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5847758.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7727..8290)
                     /gene="tag"
                     /locus_tag="OO309_002662"
     CDS             complement(7727..8290)
                     /gene="tag"
                     /locus_tag="OO309_002662"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR5847759.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            8448..9146
                     /locus_tag="OO309_002663"
     CDS             8448..9146
                     /locus_tag="OO309_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5847760.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            9375..10583
                     /locus_tag="OO309_002664"
     CDS             9375..10583
                     /locus_tag="OO309_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5847761.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10907..12598
                     /gene="eptB"
                     /locus_tag="OO309_002665"
     CDS             10907..12598
                     /gene="eptB"
                     /locus_tag="OO309_002665"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR5847762.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12690..12766
                     /locus_tag="OO309_002666"
     tRNA            12690..12766
                     /locus_tag="OO309_002666"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12724..12726,aa:Pro,seq:cgg)
     gene            13510..15117
                     /gene="dppA"
                     /locus_tag="OO309_002667"
     CDS             13510..15117
                     /gene="dppA"
                     /locus_tag="OO309_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR5847763.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            15425..16444
                     /gene="dppB"
                     /locus_tag="OO309_002668"
     CDS             15425..16444
                     /gene="dppB"
                     /locus_tag="OO309_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR5847764.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            16454..17356
                     /gene="dppC"
                     /locus_tag="OO309_002669"
     CDS             16454..17356
                     /gene="dppC"
                     /locus_tag="OO309_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR5847765.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            17367..18350
                     /gene="dppD"
                     /locus_tag="OO309_002670"
     CDS             17367..18350
                     /gene="dppD"
                     /locus_tag="OO309_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR5847766.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            18347..19351
                     /gene="dppF"
                     /locus_tag="OO309_002671"
     CDS             18347..19351
                     /gene="dppF"
                     /locus_tag="OO309_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR5847767.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(19381..20652)
                     /locus_tag="OO309_002672"
     CDS             complement(19381..20652)
                     /locus_tag="OO309_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5847768.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         5071 a         5138 c         5473 g         5243 t
ORIGIN      
        1 tgatgagctt ctcagggctg cggagatacc gcgcagtacg ttttattacc atctaaaggc
       61 tctcagcaag cctgacaagt atgcggacgt taaaaagcgt attggtgaga tttatcacga
      121 gaatagaggc cgatacggat accgtagggt aacgctgtct cttcatcgag aagggaaaca
      181 gattaaccat aaagctgttc agcgcctgat gggaaccctc tcacttaaag cagcgattaa
      241 ggtcaagcga taccgctctt acagaggaga ggtagggcaa accgccccta atgttctcca
      301 aagagatttc aaggctacgc ggccaaacga gaagtgggtt accgatgtta ctgaatttgc
      361 agtcaatggg cgcaagctgt atttgtctcc agtaatagat ctcttcaaca acgaagttat
      421 ttcttacagc ctttcggaaa gaccagtgat gaacatggtt gagaatatgc tcgatcaggc
      481 attcaaaaag cttaatcctc acgagcatcc tgttctgcac tctgaccagg gatggcagta
      541 tcgtatgaga agatatcaaa atatccttaa agaacatggt attaaacaaa gcatgtccag
      601 aaaaggcaat tgtctggata atgctgtggt ggagtgtttc tttggaacct taaagtcgga
      661 gtgtttttat cttgatgagt tcagtaatat aagcgaactg aaggatgctg ttacggaata
      721 tattgaatac tataacagca gaagaattag cctgaaatta aaaggtctga ctccaattga
      781 atatcggaat cagacctata tgcctcgtgt ataactgtcc aactttttgg ggtcagtaca
      841 aagtgaggct accctatgcc tgaacaccat aaggttagcc tcttactcgt tggaaatcaa
      901 cacagggggc tgggaatgcc gcagaaatat agattacttt ctttaatagt gatttgtttc
      961 acgcttttat ttttcacctg gatgataaga gattcactgt gtgaattgca tattaaacag
     1021 gagagttatg agctggcggc gtttttagcc tacaaattga aagagtaaga gtcttcgacg
     1081 ggaaattatt cccgccttac ttacggcgtt gcgcattctc attgcaccca tatttattct
     1141 tcacaaaaat aataatagat tttattacgc gatcgattat ttatttcctg aaaaaaaata
     1201 aaaaaatccc cgccaaatgg cagggatctt agattctgtg cttttaagca gagattacag
     1261 gctggttacg ttaccagctg ccgggccttt agcgccgctt tcgatggtga aggacacttt
     1321 ctgaccttcg tccagagatt tgtaaccatc gttctggata gcagagaagt gtacgaacac
     1381 atctttagag ccatcgtcag gagtgatgaa gccgaagcct ttgtcagcgt tgaaccattt
     1441 tacgatacca gtcattttac cggacatagt gtattacctt taataattaa gtgtgccttt
     1501 cggcgatatg gcgtgcttta cagattttga agcgttaaag gaatgtgcac tacgaggggt
     1561 atcaacgata actcttgaag ggacttgcct tactacactg ctttaatggt ctgtacgtca
     1621 aaccgttgat gtgcattaag ccactcattg gcgggtgatg caacaattat ttttcatatt
     1681 tatgattaat cggttggaaa acggtcctgt catcaggacc gtaaacagca ataaagtgga
     1741 taaaagtcta tttcatcaac tgcttactta atgccgggtt ggcttgaatc aaacgcatca
     1801 attttagttc ggcacttgaa ggcttcacgc gtctggattc ccattccttt accatggcga
     1861 ctgatacgcc caaaacccgg gcgaaatcat cgatttttaa tcctgtccct tttcataact
     1921 gctcaatttc ggtacaggac gttgttctgt gcgtcagggt aattttctgc gtttcatctt
     1981 taaaaacaat ctgttccagg ctgctcaaca gctcatgcat tggatcttta tattccattg
     2041 aaaactcctc aaatcacact gcgggatcgt gaattgcatc gaagctcatt aagaatagtc
     2101 gggaaaagca gacacagcgg ggtgaagtca gggcatgctc tggctatgat tcgattcagc
     2161 ctgtcattta gcgtgactta tagccaccta acgatctgat aaatgccgat atatctctta
     2221 tcactcctgt tttgtaaaga tttcgcaaat ctaaccttct gcgaaggcgt aaatctctgg
     2281 ctacaaaaaa ggcaacggca ctatcatttt ttaacaatga aacaacaaat taaccttgtt
     2341 tcattttctc catgatttta ccaggaagat tacgtagaaa gggtatcctg caagcctgtc
     2401 ctggactatc cttgtcacgt cagacacgcg tgtgtcgttg tgcgcttttt ttgggtgaaa
     2461 ggagtaagaa aatggcgaca ggaaagtcct gctctcgctg gtttgcgcct cttgcggcgt
     2521 tattaatggt agttagcctg agtgggtgtt ttgataaaga aggcgatcag cgtaaagcgt
     2581 ttatcgactt cctgcaaaat acagtgatgc gtagcggtga acgtctacca accctgactg
     2641 ccgatcagaa aaaacagttt ggtccttttg tctctgatta cgcgattctg tatggttatt
     2701 ctcagcaggt gaatcaggcg atggattccg gtctgcgccc ggtcgtagac agcgttaacg
     2761 caattcgcgt accgcaggat tacgttacgc aaagcggtcc gctgcgtgaa atgaatggtt
     2821 cgctgggtgt gctggcgcaa caactgcaaa atgcgaagtt gcaggccgat gctgcgcact
     2881 ctgcgctaaa acagagcgat gacctgaaac cggtatttga tcaggcattc accaaagtgg
     2941 taacgacgcc agctgatgca ttgcaaccgt tgattccggc ggcacaaact ttcacgcaac
     3001 aactggtgat ggttggagac tacattgctc agcaaggtac tcaggtgagc tttgtcgcaa
     3061 atggcatcca gttcccgact tcacagcagg caagtgaata taacaaactg attgcgccat
     3121 taccggcaca acatcaggcg tttaatcagg cctggactac agcagttact gcaacccaat
     3181 aaagagtaaa agcccgagcg attatcgggc tttacttcac gcagtcgcag cttagtccgc
     3241 gacgtgagga ttcacacagt tcttctcaac ctttccttgt aacgcatcaa tcaaattatc
     3301 cacggcacag gcggccatgc cataacgcgt ctcatgggtg gcagatccaa tatgcggtac
     3361 tgcgacgacg ttggccattg agagcaacgg cgaatctacg gacagtggct cttgttcgaa
     3421 gacatccagc ccggcggcgt gaatttcgcc tttctgcaat gctgcgatca gtgcattttc
     3481 gtcaaccacc gggccacgtc cggcattaat gaaaatggcg gaggatttca ttttggcgaa
     3541 ttgttctgcg ccaaacagat gatgcgtctc atcagttaac ggcaggatca ggcaaacgaa
     3601 atctgactct tgtaacagag tatccaaatc gcagtagcgg gcgttgaagc gttcttctgc
     3661 ttctttatgg tggcggcgcg cgttatagag gatgggcatg ttgaagccaa agtgcacacg
     3721 ttgtgccagc gccatgccga tccgtcccat cccgacaatg cccagtgttt tatggtgaac
     3781 gtcagtgccg taccagtccg ggcctatgct cgcggtccat tcgcctgctt ttacccgttc
     3841 tgctacctcc acaacccgac gagcggtaga caacaccagc gccatcagcg tatcggcgac
     3901 ggtttctgtt aatacggttg gcgtgtgcat cagcagaatt tttcgggcgg taagcgcatc
     3961 gacatcaaaa ttgtcatagc cgacggagat cgttgatgtg gcacgcagtt tcggcatttt
     4021 ttccagcaat gcggcatcta cattctcgtt tgaacccagt aaaccttcag cttctgcaaa
     4081 aattgctgca ttttgaacga cggtttgtgg gctgaggttt gccacctggt gaacggtgaa
     4141 atgcgcttgc aggcgttgca gtaaatcatc aggtaaggct ttgtagagga taacggacgg
     4201 cttcatgctt ctctccattc actgataagg cgatcacctt agcctgaaat atgatgaagg
     4261 aaaactgaag aatgccaggt gatttctgca tcacctggca tgaaagggat tacagcgggc
     4321 ttaaggtaat ttctacacgg cggttttgcg ccttaccttc tgcggtgctg ttgctggcga
     4381 ttgggttagc cgggccaagg ccctgagtac ggatgcggct ggcgtccacg ccctgggtga
     4441 tcaacgcgct ggcaacggaa tccgcacgct gctgggagag acgcatgttc aggtcgtgac
     4501 cccccgtgct gtcggtataa ccaatcacgt taaccgccgt tttcggatac tctttcagca
     4561 ccattgccac gccggtcagg gtgttagcgc ctgccggttt caggggagcg ctgctgctgt
     4621 cgaaggtcac attgttcggc atattgagga taatgttatc cccgctgcgg gttacgctaa
     4681 caccagtgcc gcgcattttg tcacgcagct tcgcttcctg cacatccatg taataaccca
     4741 cgccgccgcc cagagctgcg cctgctgctg cgccaatcag cgcgccttta ccgcgatctt
     4801 tcttcgaaga agagagcgca ccaatacccg cgcccacgag agagcccaga cctgcgccga
     4861 tagcagattt gcctgcttcg cgttcgccgg tgtaagggtt agttgtgcag ccagataccg
     4921 ccagagcgcc actcactacg gcggcaagta agataaacac gtttcttcat tgttaatcct
     4981 taataacctt tttattcttt gtcacgggtt ccgtggcggg agattatgcc gcgtgaacat
     5041 gaagattatt cctgggaata ctcggaaatt tgtaagtaat atttaactgc tcaatacatc
     5101 taacctttca ggagtcttcg gtttggccaa ctcatcctca cgatattccg ttctgactgc
     5161 cgcccactgg gggcccatgc tggttgaaac cgacggcgaa accgtgttta gctcgcgtgg
     5221 cgcgttagcc acaggaatgg aaaactcctt gcagagcgcg gttcgcgacc aggttcacag
     5281 caatacgcgg gtacgatttc caatggtgcg caaaggcttt ctcgcgtcac cggaaaaccc
     5341 gcaaggcatt cgtgggcagg atgaatttgt tcgcgtgagt tgggatgagg cgctggcagg
     5401 cggttatacc gggcatctgg gggattattc gaccggtgcg gcacaggcga tcatgccgta
     5461 tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt tggccgctgg tgctggaaca
     5521 tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat acgctgaaaa ttgcgtggaa
     5581 tgcatccgat gagcaggggc tttcttactt ttccgcactg cgtgacagtg ggaaaaagct
     5641 gatctgcatt gatccaatgc gatcggaaac cgtcgatttc tttggcgata aaatggagtg
     5701 ggtggcaccg cacatgggca ccgatgttgc gctgatgctg gggatcgccc atacgctggt
     5761 ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc accacaggtt atgccgtctt
     5821 cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa aacgccgaat gggcggcaga
     5881 gatttgtggt gttggcgcag cgaaaatccg cgagctggcg gctattttcc accaaaatac
     5941 caccatgctg atggcaggct ggggaatgca gcgccaacag tttggtgagc aaaaacactg
     6001 gatgatcgtc acgctggcag caatgttggg gcaaatcggc acacccggcg gcggttttgg
     6061 tctttcttac cattttgcca atggtggtga ccccacgcgg cgttctgcgg tgctctcttc
     6121 catgcagggc agcttgccgg gtggctgcga tgcggtggat aaaatccctg ttgcccgcat
     6181 tgttgaagca ctggaaaacc ctggtggcgc atatcaacac aacggtatga accgacattt
     6241 cccggatatt cgttttatct ggtgggcggg cggtgccaac tttactcatc atcaggatac
     6301 caatcgtctg atccgtgcct ggcaaaaacc ggagctggtg gtgatctctg aatgcttctg
     6361 gacggcggcg gcgaaacacg cggatattgt tctgcctgcg actacctctt ttgagcgtaa
     6421 tgatctcacc atgaccggtg attacagtaa tcagcatctg gtgccgatga agcaagtggt
     6481 gccgccacgc tatgaagcgc gtaatgattt tgatgttttt gccgagttga gtgaacgttg
     6541 ggagaagggc ggctatgcgc ggtttacgga aggaaaaagt cagctgcaat ggctggaaac
     6601 gttttataac gttgcccggc agcgcggggc aagccagcag gttgaattgc cgccatttgc
     6661 tgagttctgg caagccaacc agttaattga gatgccggaa aacccggaca gcgagcggtt
     6721 tattcgcttc gctgattttt gccgcgatcc gctggcgcat ccgttaaaaa ccgccagcgg
     6781 caagattgaa atcttctcac agcgtattgc cgattacggt tacccggatt gccctgggca
     6841 tccaatgtgg ctggagccgg acgaatggca gggcaatgcc gaaccagaac agttgcaggt
     6901 actttctgcc catccggcgc accgcctgca cagccagctg aattacagtt ctctgcgcga
     6961 attgtacgcg gtagcaaacc gtgagcctgt caccattcat cctgacgatg ctcaggcgcg
     7021 cggcataacc gaaggggata tggtgcgggt gtggaactcc cgtgggcaga tccttgccgg
     7081 agcggtcatt agcgagggaa ttaaacctgg cgtgatttgc attcacgaag gggcatggcc
     7141 ggatctggat ttaaccgctg acggtatttg taaaaacggc gcagtgaacg tgctgaccaa
     7201 agatctcccc agctcgcggc tggggaatgg ctgtgcgggt aatacgacgc tggcatggct
     7261 ggaaaaatac aacggtccgg aactgacact tacagcgttt gaaccaccgg ccagctcata
     7321 atccaggtgg gtagttgggt ttcatcctgc catgcgcaat cgacaatgtg aaaaccctgc
     7381 gcacggtaaa aatctatcgc cggttgattt ttttgataaa cctccagcat cagatgggga
     7441 tagcgctgct gcacatactg catcagcgcc ttaccaatac cgcgtctgac ggccttcggt
     7501 gcgacaaaca ttgctgccag aaatcggcct tccataatgc tgacaaaacc gagaagctta
     7561 ccgtcctctt cccagaccca gttttgcgcg ttggcaagat aggcatcccg caccagcgga
     7621 atgcagtcac gccagtaatt cgcttttata aagggatgcc cccaggttgt actttccagc
     7681 cacagttcga ggatcgcggg gagttctgaa cgttgcgctt cccgaatcat ggtttatttc
     7741 ccagatagca acagcagcca accacatgat cattcaccag cccacatgcc tgcataaagg
     7801 agtaacagat tgtggtgccg acaaacttaa aaccacgttt tttcagtgcc ttagataggg
     7861 cgtcggaggc ggacgtagat gtgggaattt cgctcaacgt tgtggcttgt gtcacctgtg
     7921 gctgatgatt tacaaacgac cagacaaagt cgggaaacgg ttcgccgttc tgttccattt
     7981 gcaggtacgc ccgcgcatta ccaataattg cctgaatttt ccctcgatgg cggataatcc
     8041 cggcgtcctg taccagtctt tcgacatcct cttcctgcat tgctgcgacc ttcaccggat
     8101 cgaactgatg aaagtaggcg cgatagtttt cgcgtttttt gaggacggtg atccacgata
     8161 acccagcctg ctgcccttca aagcagatca tttcgaacag ttttttactg tcagtttcag
     8221 gcacgcccca ctcattatca tggtaggcaa tataaagcgg gccctgactc acccagccgc
     8281 aacgttccat actttccctc gctatgtacc cgatcgtaaa aatttcagcc gcttttcttg
     8341 acgcatcggt gaaaaagtca atatttaata cagggctact atgcccgata tccttcatac
     8401 aatgacaata atattgccga attcggctct tccctggagt cgtgttaatg atcataaaaa
     8461 aaagcggtgg tcgctggcag ctaagcctgc tggcgagcgt ggtaatcagt gccttttttc
     8521 tcaacacagc ttacgcctgg caacaagaat atatcgttga tacgcaaccc ggacattcca
     8581 cagagcgtta cacctgggat agtgatcatc aacctgatta caacgatatt ttgtcgcaac
     8641 gtattcaaag tagccaaagg gcgctgggac tggaagtcaa tctggcggaa gaaactcctg
     8701 tggatgtgac cagcagtatg agtatgggct ggaattttcc tttatatgaa caggttacaa
     8761 ccggcccggt cgcggcatta cattacgatg gcacaaccac ctcgatgtat aacgagtttg
     8821 gcgacagtac taccacgctg accgatccgt tatggcatgc cagcgtgagt agcttaggct
     8881 ggcgtgttga ctcccggctt ggcgatctcc gaccctgggc gcaaatcagc tataaccagc
     8941 aatttggcga gaatatctgg aaggcgcaat caggcctgag ccggatgacg gcgacaaacc
     9001 agaacggcaa ctggctggat gtcaccgtag gcgctgatat gttgctcaat caaaatattg
     9061 ccgcctatgc tgcgctaact caggcagaaa ataccactaa taatagcgac tatctgtata
     9121 cgatgggggt tagcgccaga ttttaacgta acagtcacaa ttgaaaccat tgaataacaa
     9181 cagttgtggc gatagtgggt gctaacttac caaataataa atttggtgaa taattgtcgc
     9241 gtcattcatt cctgaactaa ggcatttcat tccgttctga tggcatttca tgccgttttt
     9301 ccccaagcat aaaatgcact tcgttatggt tgtcggcaga gatttttcct ttttattact
     9361 gcaggaatac tgccatgaca ccttcaaatt atcagcgtac ccgctggctg acactcatcg
     9421 gtactatcat tacccagttt gcgctggggt cggtttatac ccggagcctg tttaatggcg
     9481 cgctttctgc caagctggat gcgccggtaa gccaggtcgc tttctctttc ggcttgttaa
     9541 gtctggggct ggcaatttcg tcttctgttg cgggcaaatt gcaggaacgt tttggcgtta
     9601 aacgcgtcac cgtggcttcc ggcattttgc tgggattagg cttcttcctg accgcgcatt
     9661 ccaacaacct gatgatgctg tggttaagcg ccggtgtgct ggtcggtctg gcggatggcg
     9721 cgggttacct gctgacgctc tctaactgcg tgaagtggtt cccggagcgt aaaggtctga
     9781 tctccgcgtt cgctatcggt tcttatggtc tgggtagcct gggtttcaaa tttatcgaca
     9841 cgcagctgct cgaaacggtg ggtctggaaa aaacctttgt gatttgggga gcgattgcgc
     9901 tggtgatgat tgttttcggc gcaacgttaa tgaaagacgc accaaaacag gaagtgaaaa
     9961 ccagcaatgg tgtggtggag aaagattaca cgctggcaga gtcgatgcgt aaaccgcagt
    10021 actggatgtt agcggtaatg ttcctgactg cctgcatgag cggcctgtac gtgattgggg
    10081 tagcgaaaga tatcgcccaa agtctggcac accttgatgt ggtttccgca gccaatgcag
    10141 taacggttat ttccatcgct aacctttcag gtcgtctggt gctgggcatt ctgtctgaca
    10201 aaattgcccg tatccgtgtt atcaccattg gtcaggtgat ttcgctggtg ggtatggcgg
    10261 ccctgctgtt tgcaccatta aatgcagtga cgttctttgc agcgattgcc tgcgtggcat
    10321 ttaactttgg cggcactatt accgtctttc catcactggt cagtgagttc ttcggcctca
    10381 ataacctggc gaaaaactac ggtgtgattt acctcggttt cggtatcggt agcatttttg
    10441 gttcgattat cgcctcactg tttggcggtt tctatgtgac cttctacgtg attttcgccc
    10501 tgctgattct gtcattggcg ctttctacga cgattcgtca gccagagcag aaaatgttgc
    10561 gtgaggcgca tggctccctt taatgctcag ccgcgagcta ctgggcttca gcttccgtgt
    10621 gcatcaaacg catcaacgaa atgtataaac aatgactcgt aaaatttggg ccagttaact
    10681 ggcccttttt actgcttttt gtaacgctaa agtctctttt caaacttgca tttttgtaaa
    10741 tttgtgcttc atgcacactc tttccccaca ctttttcgct ttgctgtggt ctacttattc
    10801 gcgcgtgtag attttactta tctgactacc tccgcacttt tcccctgccg ggcctgaaaa
    10861 gccgctaagc agggtgttat cacctgtttg tccagggttt gtttgcatga gatacatcaa
    10921 atcgattaca cagcagaagc tgagcttttt gcttgcaatc tatattggcc tttttatgaa
    10981 tggcgcggtt ttttatcgcc gcttcggcag ctatgcgcac gattttaccg tctggaaagg
    11041 cgtttctgct gttgttgaac tggccgccac cgtactggtg accttctttt tactacgtct
    11101 tctatcgctg tttggccgcc gcagctggcg tattctggcg tcgctggtgg tgctcttttc
    11161 cgcaggtgcc agctattaca tgaccttcct taatgtggtc attggttatg gcatcatcgc
    11221 ttccgtcatg accaccgata tcgacctgtc aaaagaagtt gttggtctga actttattct
    11281 ctggttaatc gccgttagtg cattgcctct tatccttatc tggaataacc gctgtcgcta
    11341 caccttgctc cgacaactgc gaaccccagg gcagcgtatt cgcagcctgg tggtcgtcgt
    11401 actggcgggt attatggttt gggcaccgat tcgtttgctg gatatccagc agaagaaagt
    11461 ggagagggcg accggcgttg atttgccgag ttatggcggt gtcgtagcga actcttatct
    11521 gccatcaaac tggctttctg cgttggggct gtatgcctgg gcgcgggtcg atgaatcttc
    11581 cgataataat tcattgctta atccggcgaa gaagtttaca taccaggctc cacaaaacgt
    11641 tgatgatact tatgtcgtgt ttatcatcgg tgaaaccacg cgttgggacc atatgggtat
    11701 tttcggctat gagcgtaata ccacgccgaa actggcccag gagaaaaatc tggcggcgtt
    11761 ccgtggttac tcctgtgata ccgcaaccaa actctcactg cgttgcatgt ttgtacgtca
    11821 ggggggcgcg gaagataatc cgcagcgcac attaaaagaa cagaacattt tcgcggttct
    11881 gaagcagtta ggattcagtt ctgacctcta cgctatgcag agcgaaatgt ggttctacag
    11941 caacacgatg gcagacaaca ttgcttatcg tgagcagatt ggtgcggagc cacgtaatcg
    12001 tggcaagccg gtagatgata tgttgctggt agacgaaatg cagcaatcgc tagggcgcaa
    12061 cccggatggt aagcatctga tcattctgca taccaaaggt tcgcatttta actacaccca
    12121 gcgttatccg cgtagcttcg cgcagtggaa gccggaatgt attggtgttg atagcggctg
    12181 taccaaagcg cagatgatca actcctatga caactcggtg acctatgtgg atcactttat
    12241 ctccagcgtg attgatcagg ttcgcgataa gaaagcgatt gtgttctacg cagctgacca
    12301 cggtgagtca attaatgaac gcgagcacct gcacggcacg ccgcgtgaac tggcaccgcc
    12361 ggagcagttc cgcgtaccga tgatggtctg gatgtcagat aaatatctgg aaaatccggc
    12421 caatgcgcag gcgtttgcgc agctgaaaaa agaagccgac atgaaagtgc cacgccgtca
    12481 cgtagagctg tacgacacca tcatgggttg tcttggctat acttcaccgg atggtggaat
    12541 taacgaaaac aacaactggt gtcacatccc gcagacaaaa gtggcagcgg ctaactaagc
    12601 ggcctgctga ctttctcgcc gatcaaaagg cattttgcta ttaagggatt gacgagggcg
    12661 tatctgcgca gtaagatgcg ccccgcattc ggtgattggc gcagcctggt agcgcacttc
    12721 gttcgggacg aaggggtcgg aggttcgaat cctctatcac cgaccaaatt cgaaaagcct
    12781 gctcaacgag caggtttttt tgcatttgta gctcatgagg atgagaacct ccgggggcag
    12841 ggaggctcga ctcgacgtag gcctgataag acgcgccagc gtcgcatcag gcaacggcac
    12901 cgcacttatt gtcggatgcg gcgcgaccgc cttattcgac caacacacca cacattttcc
    12961 tgccaacttt gcgccccata tctcactatc cattaacgga tttgtgacag aaaccattgt
    13021 ctttttttat atttgcttaa atcttttttg cgttacttat ggctggcgct ggcattttcc
    13081 gctgtgcgtt ttagcgttca tggtattaat cgctcagata tccacctttt cctcagattt
    13141 ttttcattaa atgcataaaa cttaatcacc aaatgttgct aaatattaag ctgattgttc
    13201 tggttatgtt ggagtagcac aaatttctct gctgcaaata gctgtttgaa gtttttttaa
    13261 tctttgtttg tggattctca ccgttggtgt aaatcccggt tggctgtatt gacgttttca
    13321 cattctgttg acagattgta ggtcacgagg ggcattttat ggaggatccg cactgttaca
    13381 ctgatgttaa ttagtacggc atccccacct cataacgttg acccgaccgg gcaaaaaaca
    13441 aaaaaggtca ggcagcgaca acccactgca aagggttaaa acaacaaaca tcacaattgg
    13501 agcagaataa tgcgtatttc cttgaaaaag tcagggatgc tgaagcttgg tctcagcctg
    13561 gtggctatga ccgtcgcagc aagtgttcag gctaaaactc tggtttattg ctcagaagga
    13621 tctccggaag ggtttaaccc gcagctgttt acctccggca ccacctatga cgcctcttcc
    13681 gtcccgcttt ataaccgcct ggttgaattt aaaatcggca ccaccgaagt gatcccgggc
    13741 ctcgctgaaa agtgggaagt cagcgaagac ggtaaaacct ataccttcca tctgcgtaaa
    13801 ggtgtgaagt ggcacgacaa taaagaattc aaaccgacgc gcgaaatgaa cgccgatgac
    13861 gtggtgttct cgttcgatcg tcagaaaaac gcgcaaaacc cgtaccataa agtttctggc
    13921 ggcagctacg aatacttcga aggtatgggc ttgccggagc tgatcagcga agtgaaaaag
    13981 gtggacgata acaccgttca gtttgtgctg actcgaccgg aagcgccgtt cctcgctgac
    14041 ctggcaatgg acttcgcctc tattctgtca aaagaatatg ctgacgcgat gatgaaagcc
    14101 ggtacaccgg aaaaactgga cctcaaccca atcggaaccg gtccgttcca gttacagcag
    14161 taccaaaaag attcccgtat ccgctacaaa gcgtttgatg gctactgggg caccaaaccg
    14221 cagatcgata cgctggtctt ctctattacc cctgatgctt ccgtgcgtta cgcgaaattg
    14281 cagaagaatg aatgccaggt gatgccgtac ccgaacccgg cagacatcgc tcgcatgaag
    14341 caggataaat ccatcaacct gatggaaatg ccggggctga acgtcggcta cctctcgtat
    14401 aacgtgcaga aaaaaccgct ggatgacgtg aaagttcgcc aagcactaac ctacgcggtg
    14461 aacaaagacg cgatcatcaa agcggtttat cagggcgcgg gtgtatcagc gaaaaacctg
    14521 atcccgccaa ccatgtgggg ctataacgac gacgttcagg attacaccta cgatcctgaa
    14581 aaagcgaaag ccttgctgaa agaagcgggt ctggaaaaag gtttctccat cgacctgtgg
    14641 gcgatgccgg tacaacgtcc gtataacccg aacgctcgcc gcatggcgga gatgattcag
    14701 gcagactggg cgaaagtggg cgtgcaggcc aaaatcgtca cctacgaatg gggtgagtac
    14761 ctcaagcgtg cgaaagatgg cgaacatcag acggtaatga tgggctggac tggcgataac
    14821 ggggacccgg ataacttctt cgccaccctg ttcagctgcg ccgcctctga acaaggttct
    14881 aactactcaa aatggtgcta caaaccgttt gaagatctga ttcaaccggc gcgtgctacc
    14941 gacgaccata acaaacgcgt tgaactgtac aagcaagcgc aggtggtgat gcacgatcag
    15001 gctccggcac tgatcatcgc tcactccacc gtgtttgaac cggtacgcaa agaagtcaaa
    15061 ggctatgtgg ttgatccatt aggcaaacat cacttcgaaa acgtctctat cgaataatta
    15121 aaagccatac aagactgatg gcaaaggcaa aactgcctga tgtgctccgc ttatcaggcc
    15181 tacgaaaatt ctgcaatgta ttgaatttgc acgattttgt aggccggata aggcgttaac
    15241 gccgcatccg gcataaacaa agcgcacttt gtcaacaatc tgtatacccg gtggcgctgt
    15301 gcctctgcgt gcagcgccat ccggcagcaa tacttcattc tctgtccgac aagggcggga
    15361 attgatttgt gagcaataca gacacgcagt tccaggctgc gagtcactat agagaatccg
    15421 ggttatgttg cagtttattc tccgacgttt gggactcgtc atccccacgt ttatcggtat
    15481 tacccttctc acatttgcct ttgtccacat gatcccgggc gatccggtga tgatcatggc
    15541 aggcgaacgt gggatctccc ctgagcgtca cgtgcagctg ctggctgaac tcggcttaga
    15601 taaaccgatg tggcagcagt atctccatta catttggggc gtaatgcacg gcgatctagg
    15661 catttcaatg aaaagccgaa ttccagtatg ggaagagttc gtgccgcgct ttcaggccac
    15721 gctggaactt ggcgtctgcg cgatgatttt tgctacggca gtcggtattc cggttggtgt
    15781 gctggctgcg gttaaacgcg gttccatttt cgatcacact gcagttggcc tggcgctgac
    15841 cggctactcg atgccgatct tctggtgggg catgatgctg atcatgctgg tttcggtgca
    15901 ctggaacctg acgcccgtct ccggtcgcgt gagcgatatg gtgttcctcg atgactccaa
    15961 tccgttaacc ggttttatgc taatcgacac cgccatctgg ggtgaagacg gcaactttat
    16021 cgatgccgtc gcccatatga tcttgcccgc cattgtgctg ggtactattc cgctagcggt
    16081 cattgtgcgt atgacacgct cctcgatgct ggaagtgctg ggcgaggatt acatccgcac
    16141 cgcgcgcgcc aaagggctaa cccgcatgcg ggtgattatc gtccatgcgc tgcgtaacgc
    16201 gatgctgccg gtagtgaccg ttatcggcct ccaggtggga acattgctgg cgggggcgat
    16261 tctgaccgaa accatcttct cgtggcccgg tctggggcgc tggttgattg acgcactgca
    16321 acgccgcgac tatccggtag tgcagggcgg cgtattgctg gtggcgacga tgattatcct
    16381 cgtcaacttg ctggtcgatc tgctgtacgg cgtggtgaac ccgcgtattc gtcataagaa
    16441 gtaaggggac atcatgtcac aggttactga aaataaagtg attagcgcac cggtgccgat
    16501 gaccccgtta caggagttct ggcactattt taaacgcaac aaaggcgcgg tcgtcgggct
    16561 ggtttacgtc gccatcgtgc tgttcatcgc gatctttgcc aactggattg cgccttataa
    16621 cccggcggat cagttccgcg atgcgctgct cgccccgcca gcctggcagg aaggcggcag
    16681 tatggcacac ttgctgggca ccgatgacgt cggtcgtgat gtgctgtcgc ggctgatgta
    16741 cggcgcgcgt ttatcgctgc tggttggctg tctggtggtg gtgttgtcgc tgattatggg
    16801 cgttattctc ggcctgatcg ccggttactt cggcggcctg gttgataaca ttatcatgcg
    16861 cgtggtcgat atcatgctgg cgctgccaag tctgctgttg gcactggtgc tggtggcgat
    16921 tttcggcccg tcgattggta acgccgcgct ggcgctgacc ttcgttgcct tgccgcacta
    16981 tgtgcgctta acccgcgccg ccgtgctggt ggaggtgaat cgcgattacg tcaccgcctc
    17041 tcgcgtggca ggtgccgggg cgatgcgcca gatgtttatt aacatcttcc cgaactgcct
    17101 tgcgccgctg attgttcagg cgtcgctcgg tttctctaac gccattctcg atatggctgc
    17161 tcttggtttc ctcggcatgg gggcacagcc gccaacgcct gagtggggca ccatgctctc
    17221 cgacgtgttg cagttcgcgc aaagcgcctg gtgggtcgtg accttccctg gtctggcgat
    17281 cctgttgacg gtgctggcat ttaacctgat gggtgacggt ctgcgtgacg cgctcgatcc
    17341 caaactaaag cagtaagagg ttcgagatgg cgttattaaa tgtagataaa ttatcggtgc
    17401 atttcggcga cgaaagcgca ccgttccgcg ccgtagaccg catcagctac agcgtaaaac
    17461 agggcgaagt ggtcgggatt gtgggtgagt ccggctccgg taagtcggtc agttcactgg
    17521 cgattatggg gctgattgat tatccgggcc gcgtaatggc agaaaaactg gagtttaacg
    17581 gccaggattt gcagcgtatc tcagaaaaag agcgccgcaa cctggtgggt gccgaagtgg
    17641 cgatgatctt ccaggacccg atgaccagcc ttaacccgtg ctacaccgtg ggtttccaga
    17701 ttatggaagc gattaaagtg catcagggcg gcaacaaaag tacccgccgt cagcgggcga
    17761 ttgacctgct gaatcaggtc ggtattcccg atccggcttc gcgtctggat gtttacccgc
    17821 atcagctttc cggcggcatg agccagcgcg tgatgatcgc catggcgatt gcctgtcggc
    17881 caaaactgct gattgccgat gaaccgacca ccgcgctgga cgtgaccatt caggcgcaaa
    17941 tcatcgaact actgctggag ctacagcaga aagagaacat ggcgctggtg ttaattaccc
    18001 atgacctggc gctggtggcg gaagctgcgc ataaaatcat tgtgatgtat gcaggtcagg
    18061 tagtagaaac gggcgatgca cacgccatct tccatgcgcc gcgtcacccg tatactcagg
    18121 cattgctgcg tgcgctgcca gaatttgctc aggacaaaga acgtctggcg tcgttgccag
    18181 gtgttgttcc cggcaagtat gaccgcccga acggctgcct gcttaacccg cgctgcccct
    18241 atgccactga cagatgtcgc gctgaagaac cggcgctgaa tatgctcgct gatgggcgtc
    18301 agtccaaatg ccattaccca cttgatgatg ccgggaggcc cacactatga gtacgcaaga
    18361 ggccacctcg caacaaccgc tgttgcaggc tatcgacctg aaaaaacatt atccggtgaa
    18421 gaaaggcatg ttcgtgccgg aacgtctggt gaaggcgctg gacggcgttt cgtttaacct
    18481 tgaacgtggc aaaacgctgg cagtagtggg cgaatctggc tgcggtaaat cgaccctcgg
    18541 tcggttgctg acgatgatcg aaacgcccac cggcggcgag ctgtattatc aggggcagga
    18601 tctgctcaag cacgatccgc aggcgcagaa gctgcgtcgg cagaaaatcc agatcgtctt
    18661 ccagaatccc tacggttcgc tgaatccgcg taagaaagtt gggcaaattc tcgaagagcc
    18721 gctgcttatt aacaccagct taagcaaaga acagcgtcgg gaaaaagccc tgtcgatgat
    18781 ggcaaaagtc ggcctgaaaa ccgagcacta tgaccgctat ccgcacatgt tttccggcgg
    18841 acaacgtcag cgtatcgcta ttgcccgtgg tctgatgctc gacccggatg tggtgattgc
    18901 cgatgagccg gtttctgcgc tggacgtctc agtgcgtgcg caggtgctga atctgatgat
    18961 ggatttgcag caggagttgg ggctttctta tgtctttatc tcccacgacc tgtcggttgt
    19021 ggagcacatt gctgatgaag tgatggtgat gtacctaggc cgctgcgtgg agaaaggaac
    19081 gaaggaccaa atcttcaata acccgcgcca tccgtacacc caggcgctgc tctccgcgac
    19141 gccacgcctg aacccggacg atcgtcgtga acgcatcaaa ctcaccggtg aactgccaag
    19201 cccgctgaat cctccgccgg gttgcgcctt caacgcccgc tgtcgtcggc gcttcggccc
    19261 ctgcacccag ttgcagccgc agctaaaaga ctacggaggt caactggtag cttgttttgc
    19321 tgttgatcag gatgaaaatc cgcagcgtta actcacaaaa ccggggccat gcgctccggt
    19381 ttactcaatc agcttcaaca gtggcgacaa gcatagcaaa atgccgtaaa gcagaatcag
    19441 ccaggcttta aatccgcgaa gtttttccag ttgtgcgact ttatagatca ggaaaaacgg
    19501 aatgaggcac gacacaatgc catataacgg gcttcccaac tgaaagaaca ccagcactga
    19561 tacacgaaac gaaacccaaa tcgtcaacgt aatgacgata aaagcgcaga tcgccagagt
    19621 cagcatgcgt gagttaattt tcttggtatc aataattcgg cttaacagat tgagaataat
    19681 gcctttaata gcctcgtgga aaccgagata aatgccaaag aatgcggtca gtacggcaaa
    19741 gatattcagc accgtagagg tgatatgaat gatatgtcca gggatcacct gcgcggccag
    19801 tgccagtgcc gagatatttt gttcaaaggc agaaacggct tcttcgtggc taattgagaa
    19861 ggtaaacgaa aaggcaaaaa acaggatcac cgcgatgagc gtgatataac taattcggtg
    19921 ggtacgcagc gcgagccggg ttgccagtac tttatctgct tcccgtttac gataggcaat
    19981 attcattggg ttaagtacct gaataaatac cgcagaaaag aagcaaaatg gaatggtaag
    20041 caggacatcg cggaaaaaga cggaggcttg cgggaaggcg gttatattgg cgaaattcca
    20101 gtgcgggatc atcgcaaaac cgaacacgac aataatccct actttgacca ccaccattgg
    20161 cccagaaatc ttaaacagta atcgttcacc accagacgca atcgctatca gtacggcgaa
    20221 aatagcgact ttataaagta gagattgtga aagatcggca tcggttaaac cgaaggtttt
    20281 caggtacgag gcgctgtcga aaaccacgga gagagagtag ataaaaatcc cgtggataat
    20341 catcaaaaag tagataaccc cgaggaaaat tccccagttc ttccccaggt aatgactgat
    20401 aatatcggtg tagtcattac aggaatcgct ttcagaaagg gtttttaaat aaatgtcctg
    20461 cactacccag gtgacaggat aagcaatgat cgctgcggta ataaataccc aaattccctt
    20521 caagccaatt tgtactggca tcagcacggt tccggcacca atcgccatgc ctatgcataa
    20581 taaaacccag ccgaagtcgt agcgtgtaaa cggcaatttc tgattgtgtt tcgatagtgt
    20641 gttgtgctgc ataaagtcct gtccagaaag aagtcggcct cgcaaaaatg aataaaccca
    20701 gtttctcttg cgcaacaggt ggagaagtcg aatacgcagt atgtaaaaaa tgaggcaaca
    20761 gattcactgg ggaggtcgcg aggaggcgca attctacaaa attgcgcatt tcactaccag
    20821 tctggagtca gcccggatat gaactgcaac tattgcaaat aaagaaatta agctggaagg
    20881 catcgcgcaa tgaggaagat tgccgggtgc tgacagtaaa aagca
//
LOCUS       DAJTQQ010000066        20704 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000066 DAJTQQ010000000
VERSION     DAJTQQ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.066"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(42..2111)
                     /gene="glyS"
                     /locus_tag="OO309_002673"
     CDS             complement(42..2111)
                     /gene="glyS"
                     /locus_tag="OO309_002673"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR5847769.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2121..3032)
                     /gene="glyQ"
                     /locus_tag="OO309_002674"
     CDS             complement(2121..3032)
                     /gene="glyQ"
                     /locus_tag="OO309_002674"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR5847770.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3127..3426)
                     /locus_tag="OO309_002675"
     CDS             complement(3127..3426)
                     /locus_tag="OO309_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR5847771.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3601..4596
                     /gene="wecH"
                     /locus_tag="OO309_002676"
     CDS             3601..4596
                     /gene="wecH"
                     /locus_tag="OO309_002676"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR5847772.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4638..5075)
                     /locus_tag="OO309_002677"
     CDS             complement(4638..5075)
                     /locus_tag="OO309_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR5847773.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5121..5462)
                     /locus_tag="OO309_002678"
     CDS             complement(5121..5462)
                     /locus_tag="OO309_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5847774.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5631..7085)
                     /gene="xylB"
                     /locus_tag="OO309_002679"
     CDS             complement(5631..7085)
                     /gene="xylB"
                     /locus_tag="OO309_002679"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR5847775.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7157..8479)
                     /gene="xylA"
                     /locus_tag="OO309_002680"
                     /pseudo
     CDS             complement(7157..8479)
                     /gene="xylA"
                     /locus_tag="OO309_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8845..9837
                     /gene="xylF"
                     /locus_tag="OO309_002681"
     CDS             8845..9837
                     /gene="xylF"
                     /locus_tag="OO309_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5847776.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9915..11456
                     /gene="xylG"
                     /locus_tag="OO309_002682"
     CDS             9915..11456
                     /gene="xylG"
                     /locus_tag="OO309_002682"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR5847777.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11434..12615
                     /gene="xylH"
                     /locus_tag="OO309_002683"
     CDS             11434..12615
                     /gene="xylH"
                     /locus_tag="OO309_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR5847778.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12693..13223
                     /locus_tag="OO309_002684"
     CDS             12693..13223
                     /locus_tag="OO309_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR5847779.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13335..13871
                     /locus_tag="OO309_002685"
     CDS             13335..13871
                     /locus_tag="OO309_002685"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5847780.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13979..14803)
                     /locus_tag="OO309_002686"
     CDS             complement(13979..14803)
                     /locus_tag="OO309_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR5847781.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15123..17153
                     /gene="malS"
                     /locus_tag="OO309_002687"
     CDS             15123..17153
                     /gene="malS"
                     /locus_tag="OO309_002687"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5847782.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17331..18584
                     /gene="avtA"
                     /locus_tag="OO309_002688"
     CDS             17331..18584
                     /gene="avtA"
                     /locus_tag="OO309_002688"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR5847783.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18735..19208)
                     /locus_tag="OO309_002689"
     CDS             complement(18735..19208)
                     /locus_tag="OO309_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5847784.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19310..20158)
                     /gene="yiaJ"
                     /locus_tag="OO309_002690"
     CDS             complement(19310..20158)
                     /gene="yiaJ"
                     /locus_tag="OO309_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR5847785.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20358..>20704
                     /locus_tag="OO309_002691"
     CDS             20358..>20704
                     /locus_tag="OO309_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HCR5847786.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNAN"
BASE COUNT         5224 a         5268 c         5061 g         5151 t
ORIGIN      
        1 aaaaagcctg ccagatggca ggctttttaa taacggcgtt attactgcaa cagcgaaata
       61 tccgcaacgc gcaggaacag ttcgcgcagt ttctccagca tggtcagacg gttgagacgc
      121 aattctttgt catcaaccat gaccatcact ttatcgaaga aagcatcaac cggctcacgc
      181 agctctgcca gttcgaccag cgcatcctgg taacgacctt ccgcaaagta cggctccagc
      241 ttgtcacgta gcaccacaac ctgcatcgcc agtttaattt cttccggctc tttcagggta
      301 gaggcattca cgcgatcgct cagcacttcg tcagatttcg ccaggatgtt agatacacgt
      361 ttgttcgccg ccgccagtgc tgcagctgct tccagggtac ggaagtgcga taccgctttc
      421 atgcgggcat cgaaatcagc cggacgagtc ggacgacgcg ccagtaccgc ctggatggtg
      481 tcaacggtat aaccttcgtc ctgataccag gcgcggaagc gaccgagcat aaagtcgata
      541 acatcatcaa ctacgttggc attagtcagc ttatcgccat acagacgcac cgcttcttcg
      601 gtcagcgttt gcagatcaag gttgaggttc ttctcaacga taattcgcag cacgccaagt
      661 gcggcacgac gcagcgcaaa cgggtctttg tcgcctttcg gatgctgacc gataccgaag
      721 atacccgcca gagtatccat cttatcagca atcgccagcg cacaagctac cgggttggac
      781 ggcaggtcat caccggcaaa gcgcggctga tactgctcat tcagcgcgac ggctacgtct
      841 tccgcttcgc catcgtgacg cgcgtagtgc atccccataa cgccttgggt gtcggtgaac
      901 tcgaagacca tgttggtcat caggtcacac ttagacagca ggcccgcacg ggttgcgtgg
      961 ttaacgtcag cgccaatctg ttcggcgatc cagccagcca gcgcctggat gcggtcagtc
     1021 ttgtcacgca gcgtacccag ctgttgctgg aacagcacgg tttgcaggcg cggcaggtta
     1081 tcttccagac gttttttgcg gtcggtgttg aagaagaact cggcatccgc cagacgtgga
     1141 cgaacgactt tctcgttacc agagataatt tgctgcggat ctttcgattc gatgttggca
     1201 acgaagataa agttcggcag cagtttgccg tcgttcgcat acaccgggaa gtacttctgg
     1261 tcacctttca tggtgtaaac cagggcttca gccggcaccg cgaggaattt ctcttcgaat
     1321 tttgcggtca gaacgaccgg ccactccacc agcgaagcca cttcttccag caggctttcg
     1381 cttaagtcag cgttaccgcc aatcttacgc gctgcttctt cggcatcggc tttaatcttc
     1441 gctttacgtt cttcgtaatc ggcgatgact ttcccgcgct cacgcagaat ttccggatac
     1501 tgatcggcgt tgtcgatggt gaattccggt tcgcccataa agcggtggcc gcgaatcacg
     1561 cgatcggact gaatgcccag aatggttgcc ggaatgactt tgtcgcccag cagcagggtc
     1621 acggtgtgca ccggacgcac gaagtgcacg tcgcttgcac cccagcgcat cagtttcggg
     1681 atcggcagtt tcgccagcga agtcgcaacc atattcggca gcagtgcttc ggtgctttcg
     1741 cccttcacat gggcgcgata cagcagccat tcgcctttat cggtagtcag acgctcagcc
     1801 tggtcaacgg taataccaca accacgcgcc caaccttctg ccgctttgct cggttttcct
     1861 tcagcgtcga acgcctgggc aatcgccggg ccgcgttttt cgatttcacg atctggttgt
     1921 gcttccgcca ggttagctac tttcagcgcc agacgacgcg gagcagcaaa ccattgaacg
     1981 gtgccgtgtg cgaggcctgc gttatccagc tccgcagtaa agttcgcagc aaaggactct
     2041 gccaggctgc gcagtgcttt tggtggcagc tcttcagtgc cgatttccac cagaaaagtt
     2101 ttctcagaca tagccgcctc ttacttatct ttgttgcaca tcgggaagcc gagggcttca
     2161 cgggaagcgt aatatgcttc tgccactgct ttggtcaggg tgcgaatgcg cagaatgtag
     2221 cgctgacgct cggtgacgga gatggcttta cgcgcatcca gcaggttgaa gctgtgggcg
     2281 gctttcagaa tacgctcgta ggctggcagc ggcagcggat tttccagcgc cagcagctgc
     2341 tgcgcttctt tctcgtactg ctcaaagcag gtgaacagga agtccacatc cgcgtattcg
     2401 aagttgtaag tggactgctc cacttcgttc tgatggaaca cgtcgccgta ggtggtttta
     2461 cccagcgggc cgtcgctcca gactaggtcg taaacgctgt ctacgccctg aatgtacatg
     2521 gccagacgtt ccagaccgta ggtgatctcg ccggtaaccg gtttacactc cagaccacca
     2581 acctgctgga agtaagtgaa ctgcgtcact tccatgccgt ttagccacac ttcccagccc
     2641 agtccccagg cccccagagt cgggttttcc cagttatctt ccacgaaacg gatgtcgtga
     2701 atagtcgggt ccatgcccag ctctttcaga gaaccgaggt acagctcctg aatattgtcc
     2761 ggcgatggct taatgaccac ctggaactga tagtagtgct gtaaacggtt ggggttttcg
     2821 ccgtagcgac catcggtcgg gcgacgagaa ggctgaacat aagcagccgc catcggttct
     2881 ggccccagcg cgcgcagaca ggtcattggg tgagaggttc ccgcgccgac ttccatgtcc
     2941 aatggttgaa caatggtgca gccctggcga gcccagtaat cctgtaaggt caggatcaag
     3001 ccctggaagg tcctggtatc aaacttttgc atattatttc gtgctggata cgtgtggatt
     3061 taaaggaagg gatcagtata cccgctggat ggaagatata cagtacgaaa cgggaaaaag
     3121 cagggcttaa cgcatggaaa gatgcaaaaa atggccatct gcgtcaaaag aacagacaaa
     3181 ctgctcttta cggaaggtat aaccgcgcat ttcatagctt ccctggaact gttcgaaggc
     3241 ggtgacgtcg attttttgct cgccggtgtt ataacgtttt gccgcctgat ccttgcataa
     3301 cgcttccata ttcaacgaac gcagaggatc aacccttacc cgttgtgctt tctgcacggg
     3361 tgacggtgta gaacaaccca ctagcaccat aagcgccatt gccggaaaga aagcgttcat
     3421 catcattaaa ttaccgtctg gtttgcaggt gagtcttatt atttatatcg gtatagtggc
     3481 tgatatctta cgaatttcct taagcctcta taggctgtag tgataaaact cagattattc
     3541 cagtgcgcca accaaatgat ttatcggtca gacttttttt aaccagatac agagacacag
     3601 atgcagccca aaatttactg gattgataac ctgcgaggga tagcgtgttt aatggtggtg
     3661 atgattcaca ccactacctg gtatgtgacc aatgctcata gtgttagccc cgtcacatgg
     3721 gatatcgcca atgttctgaa ttctgcctct cgtgtcagcg tgccgctatt tttcatgatt
     3781 tccggctatc tcttttttgg cgaacgcagc gcccagccgc gccatttctt gcgtatcggc
     3841 ttatgtctga ttttttatag cgcaatcgca ctgctctaca ttgcgctgtt tacctccatc
     3901 aatatggagt tagcgctgaa aaacctgctg caaaagccag tgttttacca cttgtggttt
     3961 ttcttcgcga ttgcggtgat ttatctggtt tcaccgctga ttcaggtgaa gaacgtcggc
     4021 ggaaaaatgt tgctggtgct aatggtggtg attggcatta tcgctaaccc aaacacagtg
     4081 ccgcagaaaa ttgacggttt tgaatggctg ccaattaact tatatatcaa tggcgatact
     4141 ttttactaca ttctgtatgg catgttgggc cgcgctatag ggatgatgga cacacagcat
     4201 aaagcactgt cgtgggtgag cgccgcgctg tttgcgacgg gggtttttat tatctctcgc
     4261 gggacattat atgaattgca gtggcgcgga aattttgccg atacctggta tctttactgt
     4321 gggccgatgg tttttatctg cgcaatcgcg ctattgactc tggttaaaaa cacgctggat
     4381 acgcgtacca ttcgcggact tggcttaatc tcccgccatt cattgggtat atacggattc
     4441 cacgccttga ttatccatgc gctgcgcacc cggggaattg agcttaaaaa ttggccaata
     4501 ctggatatta tttggatctt ttgcgcgacg ttggcagcga gtttgttact ttctatgctg
     4561 gtacaacgaa tcgacagaaa cagattagtg agttaagtaa aagcccgttc acattggact
     4621 gaccgggctt acgtgaatta ttcgctgtat tcttcctggg taacctgtgt ttcttttggc
     4681 gggtttattc cggcatcacg aatattcttc tgcaccgcta ctgcaccaaa caagcttaag
     4741 aagaaagcca gtccataaaa acctttttca ctgagtaata atgtcgcatt ccacagacct
     4801 accatcagta gagcaacaga gataataaag acagtcaggc aggtcatata ataaatggaa
     4861 gtggtcggta tgccttcata cttgtcccga acggtctttt gataagacgc agcagaaaac
     4921 agtcctaaca ccagtacggc aaaataatac cctttttcat ttaactgcat ctctgcattc
     4981 catagcccta acaggtaggt aacgatacca ccaacgagag ctatccatga cacaatacta
     5041 aaggccggtg aataggttga tattttgttg tccatcacag tattcctttc attcctgaat
     5101 gtgtaagagc tttatgttgc tcataccgag cgaaacaaac gcatttgacc aaacaagcag
     5161 acaaaaaagg cgatggggta gatcaccatt tcataagcat tgataggtgc atttaaaaca
     5221 ccgataaaca agagaccgat agtgattaac gcaactaact ggcagatatg ggtaaaaaaa
     5281 ttatccagtt gcccgagttt ttctgcgcga agatatgcat agttaccaaa agttgctgtc
     5341 attaacacgc caagaaaata gccttttcca cttagcaatg ggcatgagat ccatagccca
     5401 accagataaa ccagcgcccc gacaacaaat aatagttttg ccacagtttt tgaggttttc
     5461 ataacgatct ccatatctac cagcgataca ttacgagtaa ccaacgaaag acaaaactga
     5521 aaaatgccat taacaaatga ttttcagaat aaattcatac taaatattaa ttaattagtg
     5581 agatatatag atgtgaatta tcccccaccc ggtcaggcag gggataacgt ttacgccatt
     5641 aatggcagaa gttgctgata gaggcgacgg aacgtttcac gtcgtggctg ataagcggca
     5701 taacgctgcg catctggtag atgcgactgt tctaacggta gctgcggcaa caactcaatg
     5761 agcgatttct ctggattcgc cgcaatctgc gccagccttg ctgcgcccag tgctggcccc
     5821 acatcccccc ccgtacggta atcgagctgc tgaccgctga tatccgccag catctgacgc
     5881 cagtactcac tacgcgcccc gccaccaatc aacgtaacac tttgcggttt aatgccgcag
     5941 gcatgcacga catccatgcc atctgccagc gcataaccca cgccttccag cactgctcgc
     6001 gccagttcat tggggccatg ttgatgagtc aaaccaaaga aaaccccctt cgcctgggga
     6061 ttattgtgtg gtgtacgctc gccggaaaga taaggcagaa accaaactgg ctcggcactt
     6121 tcatcagcct gttgagctgc agcgattaaa gctgggacat tgcacaggcc ggttaatttc
     6181 gcggcccaat ccagacacga tgctgcactc agcattacag acattaaatg ccaacgctgc
     6241 ggtagcgcat ggcaaaagct gtgtacagcg ctttctggct tgcttaagaa cccttcgctg
     6301 acagcaaaat agacccccga cgtccccagc gataacattg cctgattagc atcaaccatt
     6361 cccacaccaa ctgcaccagc tgcattgtcg ccaccgcctg cgacaactgg caccgtcgcc
     6421 ataccccacg ctttcgcaac ttcaggtaac aaagcaccag taatttcgct gccttcgtat
     6481 aatgcgggca tctggtcacg agataagtcg caagcctgca gcatgacgtc actccagtca
     6541 cgctttgcga catccagcca catggtgcca gctgcgtcag acatatcgct ggcaaactcc
     6601 cccgtcatac gcagacgcaa gtaatctttc ggtaataata ctttgtcgat ttgacggaat
     6661 atctccggct catgccgctg aacccatagc aatttaggcg cagtaaatcc gggcatcatc
     6721 aggttgccgg taatcactcg tgattgcgga actctcgctt ccagcaaagt gcactcttgc
     6781 gcacagcgcc cgtcgttcca caaaatggca gggcgcaata cccgttgttg agcatccagt
     6841 aaggttgctc catgcatctg gccggcaata cccaatgctt taacgtcctg cagagaatgc
     6901 tgatcgccca gagctttcat tgcgcgatca gttgcctgcc accactgttc cgggtcttgt
     6961 tccgaccaaa gtggatgcgg gcgcgaaacg gtcagctttt ccgtttgcga agcaaccacc
     7021 tcaccctgct cgttgagcaa aataactttt acgcccgagg tgccaagatc tatcccgata
     7081 tacatatcga tcgttcctta aaaaaatgcc cggtatcgct accgataacc gggccaacgg
     7141 actgcacagt tagccgttat ttgtcgaaca gataatgatt taccagattt tccagttgct
     7201 cctggcgacc actctgatgc accggagaca aattatgttc ctgagcatat ttggctaaat
     7261 ctgccagtga catttggcct ttcaggattt gctggcccaa ttcgctattc cagccggaat
     7321 aacgctgcgc gatgcgttta tccagctcgc catcttcaat catgcgcgct gcaattttca
     7381 gcgccagtgc catcgtatcc atcgcgccga tatgaccgta aaacagatca tatttatcag
     7441 tactttgacg acgtactttg gcatcgaagt tcagaccacc ggtggtgaaa ccgcctgctt
     7501 tgagaatttc atacatcacc agcgcattct cttccacact gttcgggaac tggtcggtgt
     7561 cccagcccag ttgcgcatcg ccacggttgg cgtcgacaga accgaacagg ccaagcgcaa
     7621 tggcggtggc tatttcatga tggaaagagt gacctgccag cgtcgcgtgg ttagcttcaa
     7681 tgttcagttt aatctctttt tccagaccaa actgtttcag gaagccatag accgtcgcgg
     7741 catcgtaatc atattgatgt ttagtcggtt cttgcggttt cggttcgata agcaacgtgc
     7801 cctagaagcc gattttatgt ttatgctcaa ccaccatctg cataaagcgg cccagttgtt
     7861 cacgctcctg acgcaagtcg gtatttaaca gcgtttcgta accttcacga ccaccccaca
     7921 ggacatagtt ttcaccgccc aatttatggg ttgcttccat cgctgtaaca acttgcgttg
     7981 ccgcccagct gaagacttca ggatctgggt tcgtcgccgc acccgcgcca tagcgagggt
     8041 ttgtaaagca gttagcggtt ccccacagca gcttcacgcc gctctcttct tgcttggctg
     8101 ccaggacatc aaccatttgc gcaaaattat tgatgtactc ttttaacgac gcgccctcag
     8161 gggaaacatc cacatcgtgg aagcaataaa atggcacatg taacttgtgg aaaaactcaa
     8221 atgcgacatc tgctttacgc ttcgccaacg ccagtgcctc accaggctgc tgccacggac
     8281 gattaaacgc ccccacacca aacatgtccg ccccgttcca gcagaaggtg tgccagtagc
     8341 aggcggcaaa acgcaagtgc tcttccatac gcttacccaa caccagttcg tcgggattgt
     8401 agtgacggaa tgctaacggg tttgaggatt ttgagccttc ataacgaacg cgatcgagct
     8461 ggtcaaaata ggcttgcata ttgaactcca taatcaggta atgccgcggg tgatggatga
     8521 tgtcgtaata ttgggcactc cctttcaatt gctcaattat gttatttcac actgctattg
     8581 agataattca caagtgtgcg ctcgctcgca aaataaaatg ggatgatgaa aatgggtaat
     8641 tcctcgaaga taaaaatgca ataagtacaa ttgcgcaaca aaagtaagat ctcggtcata
     8701 aatcaagaaa taaaccaaaa atcgtaatcg aaagataaaa atctgtaatt gttttcccct
     8761 gtttagttgc taaaaattgg ttacgtttat cgcggtgatt gttacttatt aaagctgtcc
     8821 tctaactaca gaaggcccta caccatgaaa ataaagaaca ttctactcac cctttgcacc
     8881 tcacttctgc ttaccaacgt tgcggcacac gccaaagaag tcaaaatagg tatggcgatt
     8941 gatgatctcc gtcttgaacg ctggcaaaaa gatcgagata tttttgtgaa aaaggcagaa
     9001 tctctcggtg cgaaagtatt tgtacagtct gcaaatggca atgaagaaac acaaatgtcg
     9061 cagattgaaa acatgattaa ccggggtgtc gatgttcttg tcattattcc gtataacggt
     9121 caggtattaa gtaacgttgt aaaaaaagcc aaacaagaag gtattaaagt attagcttac
     9181 gaccgtatga ttaacgatgc ggatatcgat ttttatattt ctttcgataa cgaaaaagtc
     9241 ggtgaactgc aggcaaaagc cttggtcgat attgttccgc aaggtaatta cttcctgatg
     9301 ggcggctcgc cggtagataa caacgccaag ctgttccgcg ccggacaaat gaaagtgtta
     9361 aaaccttacg ttgattctgg aaaaattaaa gtcgttggtg accaatgggt tgatggctgg
     9421 ttaccggaaa acgcattgaa aattatggaa aacgcgctaa ccgccaataa taacaaaatt
     9481 gatgctgtag ttgcctcaaa cgatgccacc gcaggtgggg caattcaggc attaagcgcg
     9541 caaggtttat cagggaaagt agcaatttcc ggccaggatg cggatctcgc aggtattaaa
     9601 cgtattgctg ccggtacgca aactatgacg gtgtataaac ctattacatt gctggcaaat
     9661 actgccgcag aaattgccgt tgaattgggc aatggtcagg agccaaaagc agatacctca
     9721 ctgaataatg gcctgaaaga tgtcccctcc cgcctcctga caccgatcga tgtgaataaa
     9781 aacaacatca aagatacggt aattaaagac ggattccaca aagagagcga gctgtaagcg
     9841 ttacgccccg gcgcggagcg ggggcgtgat ttctctccat gccgcgtgaa tgaattggct
     9901 taggtggagt cgttatgcct tatctacttg aaatgaagaa cattaccaaa accttcggca
     9961 gtgtgaaggc gattgataac gtcagcttgc ggttgaatgc tggcgaaatc gtctcactct
    10021 gtggggaaaa tgggtctggt aaatcaacgc tgatgaaagt gctgtgtggt atttatcccc
    10081 atggctccta cgaaggcgaa attatttttg cgggagaaga gattcaggcg agtcacatcc
    10141 gcgataccga acgcaaaggt atcgccatca ttcaccagga attggccctg gtgaaagaat
    10201 tgaccgtgct ggaaaatatc ttcctgggta acgaaataac ccacaatggc attatggatt
    10261 atgacctgat gacgctacgc tatcagaagc tgctcgcaca ggtcagttta tccatttcac
    10321 ctgatacccg cgttggcgat ttagggcttg ggcaacaaca actggttgaa attgccaagg
    10381 cacttaataa acaggtgcgc ttgttaattc tcgatgaacc gacagcctca ttaactgagc
    10441 aggaaacgtc ggttttactg gatattattc gcgatctaca acagcacggt atcgcctgta
    10501 tttatatttc gcacaaactc aacgaagtca aagcgatttc cgatacgatt tgcgttattc
    10561 gcgacggaca gcacattggt acgcgtgatg ctgccggaat gagtgaagac gatattatca
    10621 ccatgatggt cgagcgagag ttaaccgcga tttaccctaa tgaaccacat accaccggag
    10681 atgaaatatt acgtattgaa catctgacgg catggcatcc ggtcaatcgt catattaaac
    10741 gagttaatga tgtctcgttt tccctgaaac gtggcgaaat actgggtatt gccggactcg
    10801 ttggtgccgg acgtaccgag accattcagt gcctgtttgg cgtgtggccc ggacaatggg
    10861 aaggaaaaat ttatattgat ggcaaacagg tagatattcg taactgtcag caagccatca
    10921 cccaggggat tgcgatggta cccgaagaca gaaagcgcga cggcatcgtt ccggtaatgg
    10981 cggttggtaa aaatattacc ctcgccgcac tcaataaatt taccggtggc attagccagc
    11041 ttgatgacgc ggcagagcaa aaatgtattc tggaatcaat tcagcaactc aaagttaaaa
    11101 cgtcgtcccc cgaccttgct attggacgtt tgagcggcgg caatcagcaa aaagcgatcc
    11161 tcgcccgctg tctgttactt aacccgcgca tcctcattct tgatgaacct accaggggta
    11221 ttgatattgg cgcgaaatat gagatctaca aattaattaa ccaactcgtc cagcagggta
    11281 ttgccgttat tgtcatctct tccgaattac ctgaagtgct cggccttagc gatcgtgtac
    11341 tggtgatgca tgaagggaaa ctaaaagcca acctgataaa tcataacctg actcaggagc
    11401 aggtgatgga agccgcattg aggagcgaac atcatgtcga aaagcaatcc gtctgaagtg
    11461 aaattggccg taccgacatc cggtggcttt tctgggctga aatcactgaa tttgcaggtc
    11521 ttcgtgatga ttgcagctat catcgcaatc atgctgttct ttacctggac caccgatggt
    11581 gcctacttaa gcgcccgtaa cgtctccaac ctgttacgcc agaccgcgat taccggcatc
    11641 ctcgcggtag gaatggtgtt cgtcataatt tctgctgaaa tcgacctttc cgtcggctca
    11701 atgatggggc tattaggtgg cgtcgcggcg atttgtgacg tctggttagg ctggcctttg
    11761 ccacttacca tcattgtgac gttggttctg ggactgcttc tcggtgcctg gaacggatgg
    11821 tgggtcgcgt accgcaaagt cccttcattt attgtcaccc tcgcgggcat gttggcattt
    11881 cgcggcatac tcattggcat caccaacggc acgactgtat cccccaccag cgccgcgatg
    11941 tcacaaattg ggcaaagcta tctccccgcc agtaccggct tcatcattgg cgcgcttggc
    12001 ttaatggctt ttgttggttg gcaatggcgc ggaagaatgc gccgtcaggc tttgggttta
    12061 cagtctccgg cctctaccgc agtagtcggt cgccaagctt taaccgctat catcgtatta
    12121 ggcgcaatct ggctgttgaa tgattaccgt ggcgttccca ctcctgttct gctgctgacg
    12181 ttgctgttac tcggcggaat gtttatggca acgcggacgg catttggacg acgcatttat
    12241 gccatcggcg gcaatctgga agcagcacgt ctctccggga ttaacgttga acgcaccaaa
    12301 cttgccgtgt tcgcgattaa cggattaatg gtagccatcg ccggattaat ccttagttct
    12361 cgacttggcg ctggttcacc ttctgcggga aatatcgccg aactggacgc aattgcagca
    12421 tgcgtgattg gcggcaccag cctggctggc ggtgtgggaa gcgttgccgg agcagtaatg
    12481 ggggcattta tcatggcttc actggataac ggcatgagta tgatggatgt accgaccttc
    12541 tggcagtata tcgttaaagg tgcgattctg ttgctggcag tatggatgga ctccgcaacc
    12601 aaacgccgtt cttgattttg ataaaaattt tctcaaagcc ggttacgtat taccggtttt
    12661 gagtttttgc atgattcagc aggaaaagaa ccatgtttac taaacgtcac cgcatcacat
    12721 tactgttcaa tgccaataaa gcctatgacc ggcaggtagt agaaggcgta ggggaatatt
    12781 tacaggcatc acaatcagaa tgggatattt tcattgaaga agatttccgc gcccgcattg
    12841 ataaaatcaa ggactggtta ggagatgtcg tcattgccga cttcgacgac aaacagatcg
    12901 agcaagcgct ggctgatgtc gacgtcccca ttgttggggt tggcggctcg tatcaccttg
    12961 cagaaagtta cccacccgtt cattacattg ccaccgataa ctatgcgctg gttgaaagcg
    13021 catttttgca tttaaaagag aaaggcgtta accgctttgc tttttatggt cttccggaat
    13081 caagcggcaa acgttgggcc actgaacgcg aatatgcatt tcgtcagctt gtcgccgaag
    13141 aaaagtatcg cggagtggtt tatcaggggt tagaaaccgc gccagagaac tggcaacacg
    13201 cgcaaaatcg gctggcagac tgactacaaa cgctgccacc gcaaaccggg attattgccg
    13261 ttactgacgc ccgagcgcgg catattctgc aagtatgtga acatctacat attcccgtac
    13321 cggaaaaatt atgcgtgatt ggcatcgata acgaagaact gacccgctat ctgtcgcgtg
    13381 tcgccctttc ttcggtcgct cagggcgcgc ggcaaatggg ctatcaggcg gcaaaactgt
    13441 tgcatcgatt attagataaa gaagaaatgc cgctacagcg aattttggtc ccaccagttc
    13501 gcgtcattga acggcgctca acagattatc gctcgctgac cgatcccgcc gttattcagg
    13561 ccatgcatta cattcgtaat cacgcctgta aagggattaa agtggatcag gtactggatg
    13621 cggtcgggat ctcgcgctcc aatcttgaga agcgttttaa agaagaggtg ggtgaaacca
    13681 tccatgcaat gattcatgcc gagaagctgg agaaagcgcg cagtctgctg atttcaacca
    13741 ccttgtcgat caatgagata tcgcaaatgt gcggttatcc atcgctgcaa tatttctact
    13801 ctgtttttaa aaaagcatat gacacaacgc caaaagagta tcgcgatgta aatagcgagg
    13861 tcatgttgta gcgatgatga gaattgtcgg cgtcacatca gggaactcag cacagccacc
    13921 tgatgcgagc aaaagcctta tccgaccgac aaatcagata aggctcgagg aaatgaaatt
    13981 acatatgcgc cgcgattaac cgttggttat cctggtacat tgcgaacagg tagttgttat
    14041 aactcttccc tttggtcgag tagcccttca gcttgtgaat catcgctgtg gcagtcactt
    14101 cctgatccgc tttacgcagc tgtgcacgcg atttacggaa cgaagagtaa gccgggtgcg
    14161 tattcaggtt agtgacatag gcgctcaccg attctttgac agaactaaac tgtgagtacc
    14221 ctttcacttt acccggcgca ttggtacaac gtcctttcat gcatttcatg ccgaacagat
    14281 tgttattgtt gcgcgccagc ttcgacgttc cccaaccgct ttctgctgca gccatcgtcg
    14341 ccaccatact ggtagggata atgtctacgc gttcaagcaa ggtattccac gggatttttc
    14401 gcgtattacc agaccacttc accttgtagc gtttggcgat gtctttcaga cgcgcacgct
    14461 cagcaggtgc ccattggccc tgatactgtt ttgaaatcag ccagttacgt tctgcagtaa
    14521 tggctgcatt ttggctggta atgtaaggca ttacggtccg gagaaacgcc tttttccttg
    14581 gtgttccgga agggtatttt cgcaaatcag gaagtgaact actctttgca ctattgcgag
    14641 aatactcttg tttactgctt acctgtttat tactagcttt agttaagtgg gacttttgac
    14701 tcgctgttgt tgtgtgcgtc ttcgctaaca cctcactcga aaacaccaga gtgagtaaca
    14761 taagaatcat cgccccatat cgtcgtatgg gagtcaaaat catcaggtct cctggtcgga
    14821 tttaatcatt ccaacacctt atatttttca caaatttgag agttgaatct caaatcatat
    14881 caaaaatagc tgtcaagagc accccaagga atagtccaaa tctgaaacta tgtcacgagt
    14941 taacgattca gattggcgct aaatcgcaga aaatgtgggg gttatcgcaa aattcagccg
    15001 ttttttgcgc gcgatcgctc acccttgctt ctcatcctgt ggacttaccg ctcagggatg
    15061 agttttgtct ggcttatcgc tggcaaactg tctgaaatcg cagcaataag gactcatcca
    15121 ccatgaaact cgccgcctgt tttctgacac tccttcctgg cttcgccgtt gccgccagct
    15181 ggacttctcc ggggttccct gcctttagcg aacagggaac gggaacattt gtcagccacg
    15241 cgcagttgcc caaaggtacg cgtccactca cgctaaattt tgaccaacag tgctggcagc
    15301 ctgcagatgc gataaaactc aatcagatgc tttccctgca accttgtagc aacacgccgc
    15361 ctcaatggcg attgttcagg gacggcaaat atacgctgca aatagacacc cgctccggta
    15421 cgccaacatt gatgatttcc atccagaacg ccgccgaacc ggtagcaaac ctggtccgtg
    15481 aatgcccgaa atgggatgga ttaccgctca cgctggatgt cagcgccact ttcccggaag
    15541 gagccgccgt acgggattat tacagccaac aaattgcgat agtgaagaac ggtcaaataa
    15601 cgttacaacc cgctgctacc agcaacggtt tactcctgct ggaacgggca gaaactgaca
    15661 catccgcccc tttcgactgg cataacgcca cggtttactt tgtgctgaca gatcgtttcg
    15721 aaaacggcga tcccagtaat gaccagagtt acggacgtca taaagacggt atggcggaaa
    15781 ttggcacttt tcacggcggc gatttacgcg gcctgaccaa caaactggat tacctccagc
    15841 agttgggcgt taatgcttta tggataagcg ccccatttga gcaaattcac ggctgggtcg
    15901 gcggtggtac aaaaggcgat ttcccgcatt atgcctacca cggttattac acacaggact
    15961 ggacgaatct tgatgccaat atgggcaacg aagccgatct acggacgctg gttgatagcg
    16021 cacatcagcg cggtattcgt attctctttg atgtcgtgat gaaccacacc agctatgcca
    16081 cgctggcgga tatgcaggag tatcagtttg gcgcgttata tctttctggt gacgaagtga
    16141 aaaaaacgct gggtgaacgc tggagcgact ggaaacctgc cgccggacaa acctggcata
    16201 gctttaacga ttacattaat ttcagcgaca aaacaggctg ggataaatgg tggggaaaaa
    16261 actggatcag aacggatatc ggcgattacg acaatcctgg attcgatgat ctcactatgt
    16321 cgctggcctt tttgccggat atcaaaaccg aatcaactac cgcttctggt ctgccggtgt
    16381 tctataaaaa caaaacggat actcacgcta aagtcatcga aggctttaca cctcgcgatt
    16441 acttaaccca ctggttaagt cagtgggtcc gcgactatgg gattgatggt tttcgggtcg
    16501 ataccgccaa acatgttgag ttgcccgcct ggcagcaact gaaaaccgaa gccagctccg
    16561 cgcttcgcga atggaaaaaa actaaccccg acaaagcatt agatgacaaa cctttctgga
    16621 tgaccggtga agcctggggc cacggcgtga tgcaaagtga ctactatcgc cacggcttcg
    16681 atgcgatgat caatttcgat tatcaggagc aggcggcgaa agcagtcgat tgtctggcgc
    16741 agatggatac gacctggcag caaatggcgg agaaattgca gggtttcaac gtgttgagct
    16801 acctctcgtc gcatgatacc cgtctgttcc gtgaaggggg cgacaaagca gcagagttat
    16861 tactattagc gccaggcgcg gtacaaatct tttatggcga tgaatcctcg cgtccgttcg
    16921 gtcctacagg ttctgatccg ctgcaaggta cacgttcgga tatgaactgg caggatgtta
    16981 gcggtaaatc tgccgccaac gtcgcgcact ggcagaaaat cagccagttc cgcgcccgcc
    17041 atcccgcaat tggcgcgggc aaacaaacga cactttcgct gaagcagggc tacggctttg
    17101 ttcgtgagca tggcgacgat aaagtgctgg tcatctgggc tgggcaacag tgacttttcc
    17161 ggcttcccgt tcgtcagtac ctcgggaagc cgccaaccag gataaaatgt cagccctaat
    17221 cagcgttgcg ggataaagca ccgctcactc ttcaacagac cgatttgcac cccagcaaat
    17281 gtagcgttat tgttaccttc cttgctacag agttcgacag atatcccgct atgacattct
    17341 ccctttttgg tgacaaattt acccgccact ccggcattac gctgctgatg gaagatctga
    17401 acgacggttt acgcacgcct ggcgcgatta tgctcggcgg cggtaatccg gcgcagatcc
    17461 cggaaatgca ggactacttc cagacgctac tgaccgacat gctggaaagt ggcaaagcga
    17521 ctgatgcact gtgtaactac gacggtccac aggggaaaac ggagctactc acactgcttg
    17581 ccggaatgct gcgcgagaag ttgggttggg atatcgaacc acagaatatt gcactaacaa
    17641 acggcagcca gagcgcgttt ttctacttat ttaacctgtt tgccggacgc cgtgccgatg
    17701 gtcgggtcaa aaaagtgctg ttcccgctgg caccggaata cattggctac gctgacgccg
    17761 gactggaaga agatctgttt gtctctgcgc gtccgaatat tgaactgctg ccggaaggcc
    17821 agtttaaata ccacgtcgat tttgagcatc tgcatattgg cgaagaaacc gggatgattt
    17881 gcgtctcccg gccgacgaat ccaacaggta atgtgattac tgacgaagag ttgctgaagc
    17941 ttgacgcgct ggcgaatcaa cacggcattc cgctggtgat tgataacgct tatggcgtcc
    18001 cgttcccggg tatcatcttc agtgaagggc gcccgctatg gaatccgaat atcgtactgt
    18061 gcatgagtct ttccaagctg gggctgcctg gctcccgctg cggcattatc atcgccaatg
    18121 aaaaaatcat cactgccatc accaatatga acggcattat cagcctggca cctggcggta
    18181 ttggtccggc gatgatgtgt gaaatgatta agcgtaacga tctgctgcgc ctgtctgaaa
    18241 cagtcatcaa accgttttac taccagcgtg ttcaggaaac tatcgccatc attcgccgct
    18301 atttaccgga aaatcgctgc ctgattcata aaccggaagg agccattttc ctctggctat
    18361 ggtttaagga tttgcccatt acgaccgagc agctctatca gcgcctgaaa gcacgcggcg
    18421 tgctgatggt gccggggcac aacttcttcc cgggactgga taaaccgtgg ccgcatacgc
    18481 atcaatgtat gcgcatgaac tacgtaccag agccggagaa aattgaggcg ggggtgaaga
    18541 ttctggcgga agagatagaa agagcctggg ctgaaagtca ctaaaaattg cctgatgcgc
    18601 tacgcttatc aggcctacat gatttctgca atatattgaa tttggaagaa tttgtaggcc
    18661 ggataaggcg tttacgccgc atctggcata aacaaagcgc actattccgg cattgagaaa
    18721 cgccggaaaa cgtttcaaac aggctgctgc tgtagccgat gccgctgcac tttctcgaca
    18781 tccatgcact gcaacgcctt tgtcgggcag gcttcaacac aggccggccc cgtctcccga
    18841 tgccagcaca gatcgcactt aatcgccctc gcctttttgc gcgaagaaac gacctccatc
    18901 gcaccaaacg ggcaagccag catacagctt ttacagccaa tgcaacgtgt ttgttcaacg
    18961 aaaatatgcc catgttcgcg gcttatcgcg tcaacagggc agacattcgc gcacggtgca
    19021 tcttcacact gatgacaggc tactggcgtg gtccagctgt ggtctttaat gacacgaata
    19081 cgggaaataa actcgtctgg tgacaacgca gcgcaatcct gattctcctg atgcgacact
    19141 gcgcaagcca cttcacaggt acggcaaccg atacatttcg tcgcatccgc aataataaac
    19201 cggttcatga tcattccttt gcactaaaac agcagagtgc cagagagcgc gtccctactg
    19261 ctttgatcca acgaaggaaa tgttaaagtt ttggcacttg tccaaagcgt tatgtgatcg
    19321 cgcccaggtc atccctgaca gtaaatccca gttcattaga aatagcctgc gcggtttcac
    19381 gcagtggttt caggagattt ttctctccca cctgtttcag acgtgatgtc gaaagcgaaa
    19441 tcgacacggc gtacggcacc cgcccatgaa tatcaaacac cggaacagca atacaggaga
    19501 cgccgagttc gttttcttct ctgtccatcg ccgctccgct ttcacgaatg tgcgccagtt
    19561 cgtcgaacat cgcgggcagc tcggtaatgg tattgcgggt taacggctgg atctcatgct
    19621 gatggttttc ccagtatgac ttcacgtagt ccgggtgacc aaacgccatg tagatcttgc
    19681 ccattgcgga acagtagagc ggcatatgct ggccaatata ggcacgggtt cgcagcatcc
    19741 cggttgtggg ttccagctta taaatcaaaa tagcgtgatc gtcttcgcgg ctggagaagt
    19801 taatggtttc accagtggcg atgttcagtg cctcaagatg cggagcggcg atatgaatga
    19861 tattcagcga agacagcgcc ttctgcccga cggcaataaa tttggtagtc aggcggtaac
    19921 tccctgcggc gggcgcggtg gtcacatagc cgcaggactg taatccctgc aataagcgat
    19981 ggacggtact cttatttaaa ccagccagct ccgaaagatg cgccaacgga caaccgtttg
    20041 gatagttgct caaaatctca atcagcatca acccgcgaaa cagactctgg cttccggctg
    20101 gacgctcttt ttcctgcgcc atctcgttct ctttttttcc catcacttct ttccccattt
    20161 tgtcgcgtcc tgatggtagc gcaaagtgtg ccgtagttca cgatctcgac agataattta
    20221 taaccacttg atttttatgt cttttgaaat tcatcaatca gattgctttg ttaaaaagtg
    20281 atcgatatat ttgaaatcta gttccgcata ttgaaatttt aagccaaaaa agcgatcaaa
    20341 aaacaaggaa gcctaggatg aaagtgacat ttgagcagtt aaaagcagcc tttaatcggg
    20401 tcttaatttc acgcggcgtt gacagcgaaa cggctgacgc ctgtgcagag atgttcgccc
    20461 gcaccaccga atccggcgtt tattctcacg gcgttaatcg tttccctcgt ttcattcaac
    20521 aactggaaaa cggcgatatc attcctgatg cccaacccaa acgtataacc agcctcggcg
    20581 caattgaaca gtgggatgcc cagcgttcga tcggcaacct gacggcgaaa aagatgatgg
    20641 atcgcgccat tgaactggct gccgatcacg gtattggtct ggtggcactt cgtaatgcca
    20701 acca
//
LOCUS       DAJTQQ010000067        20602 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000067 DAJTQQ010000000
VERSION     DAJTQQ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.067"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            86..385
                     /locus_tag="OO309_002692"
     CDS             86..385
                     /locus_tag="OO309_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5847787.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            395..919
                     /gene="ygaP"
                     /locus_tag="OO309_002693"
     CDS             395..919
                     /gene="ygaP"
                     /locus_tag="OO309_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR5847788.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(966..1370)
                     /gene="stpA"
                     /locus_tag="OO309_002694"
     CDS             complement(966..1370)
                     /gene="stpA"
                     /locus_tag="OO309_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR5847789.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2038..2487
                     /gene="alaE"
                     /locus_tag="OO309_002695"
     CDS             2038..2487
                     /gene="alaE"
                     /locus_tag="OO309_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR5847790.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2524..2868)
                     /locus_tag="OO309_002696"
     CDS             complement(2524..2868)
                     /locus_tag="OO309_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR5847791.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3020..3250
                     /locus_tag="OO309_002697"
     CDS             3020..3250
                     /locus_tag="OO309_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5847792.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3597..3842
                     /gene="nrdH"
                     /locus_tag="OO309_002698"
     CDS             3597..3842
                     /gene="nrdH"
                     /locus_tag="OO309_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR5847793.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3839..4249
                     /gene="nrdI"
                     /locus_tag="OO309_002699"
     CDS             3839..4249
                     /gene="nrdI"
                     /locus_tag="OO309_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR5847794.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4222..6366
                     /gene="nrdE"
                     /locus_tag="OO309_002700"
     CDS             4222..6366
                     /gene="nrdE"
                     /locus_tag="OO309_002700"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR5847795.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6376..7335
                     /gene="nrdF"
                     /locus_tag="OO309_002701"
     CDS             6376..7335
                     /gene="nrdF"
                     /locus_tag="OO309_002701"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR5847796.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7690..8892
                     /gene="proV"
                     /locus_tag="OO309_002702"
     CDS             7690..8892
                     /gene="proV"
                     /locus_tag="OO309_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR5847797.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8885..9949
                     /gene="proW"
                     /locus_tag="OO309_002703"
     CDS             8885..9949
                     /gene="proW"
                     /locus_tag="OO309_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR5847798.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10007..10999
                     /gene="proX"
                     /locus_tag="OO309_002704"
     CDS             10007..10999
                     /gene="proX"
                     /locus_tag="OO309_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR5847799.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11191..12370
                     /locus_tag="OO309_002705"
                     /pseudo
     CDS             11191..12370
                     /locus_tag="OO309_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12494..13231
                     /gene="ygaZ"
                     /locus_tag="OO309_002706"
     CDS             12494..13231
                     /gene="ygaZ"
                     /locus_tag="OO309_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR5847800.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13221..13556
                     /gene="ygaH"
                     /locus_tag="OO309_002707"
     CDS             13221..13556
                     /gene="ygaH"
                     /locus_tag="OO309_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR5847801.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13647..14177
                     /gene="emrR"
                     /locus_tag="OO309_002708"
     CDS             13647..14177
                     /gene="emrR"
                     /locus_tag="OO309_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR5847802.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14304..15476
                     /gene="emrA"
                     /locus_tag="OO309_002709"
                     /pseudo
     CDS             14304..15476
                     /gene="emrA"
                     /locus_tag="OO309_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15493..17031
                     /gene="emrB"
                     /locus_tag="OO309_002710"
     CDS             15493..17031
                     /gene="emrB"
                     /locus_tag="OO309_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR5847803.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17095..17610)
                     /gene="luxS"
                     /locus_tag="OO309_002711"
     CDS             complement(17095..17610)
                     /gene="luxS"
                     /locus_tag="OO309_002711"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR5847804.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17760..19316)
                     /gene="gshA"
                     /locus_tag="OO309_002712"
     CDS             complement(17760..19316)
                     /gene="gshA"
                     /locus_tag="OO309_002712"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5847805.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19389..19817)
                     /locus_tag="OO309_002713"
     CDS             complement(19389..19817)
                     /locus_tag="OO309_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5847806.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19814..20380)
                     /gene="yqaB"
                     /locus_tag="OO309_002714"
     CDS             complement(19814..20380)
                     /gene="yqaB"
                     /locus_tag="OO309_002714"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR5847807.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4972 a         5270 c         5146 g         5214 t
ORIGIN      
        1 tttactctca tttacgctgc tgaggctggt atatgttgca tttttagttt agagtttact
       61 taatttagaa aatacttaaa taaatatgac tgaactcgcg caattacagg ccagtaccga
      121 acaggcagcg gccttattga aagcaatgag ccaccctaaa cggttgctga ttctgtgcat
      181 gcttagcggt tcccccggca ccagcgcggg agagctgacg cgcattaccg gactgagcgc
      241 ctctgcgaca tcacagcatc tcgctcgtat gcgggacgaa gggcttatca acagccaacg
      301 ggatgcccaa cgcattctat attccattaa aaatgaggcg gtaaatgcca ttatcgccac
      361 cctgaaaaat gtctattgtc cgtaaggagt cactatggct ttgacaacca tttcgccgca
      421 tgatgcacaa gaattaatcg cacgcggcgc aaagttaatc gatattcgtg acgctgatga
      481 atatcttcgt gaacatattc ctgaagcaga tctggctcca ttatccgtgc tggaacagtc
      541 aggtcttcca cctaaattac gtcgcgagca aattatattc cactgccagg caggtaaacg
      601 caccagtaat aacgccgata aattagcagc gattgccgcc cccgcagaaa tatttttact
      661 cgaagatggg attgatggct ggaaaagagc tggattgcca gtagcggtaa ataaatctca
      721 acccttgccg ttaatgcgcc aggtgcagat cgctgcgggt ggtttaatat taaacggcgt
      781 tgtactgggt tataccgtaa atagcggttt cttcttatta agtggctttg ttggtgccgg
      841 gttactgttt gcaggaatca gcggtttttg tggaatggca aggttgttag ataagatgcc
      901 gtggaaccaa cgagcttgag aagcgacgcc ggacgcgccc tagcagcgac atccggcctc
      961 agtaattaga tcaggaaatc gtcgagagat ttaccttctg ccagcgcctg agcaattggc
     1021 ttcggtgtac ggccctgacc ggtccaggtt ttagtttcac cgttaacatc agtgaattta
     1081 tatttcgccg gacgcggctg gcgtttttta ccagcacgtg gtgcagcagc agagctatta
     1141 cccaataact cttccgggtt aattccgtca gctttcatca gctccagcca ggtgctaatt
     1201 ttttcctgac gctcagccag ttcacgctgc tgctgttctt cttcttcacg tctttcttta
     1261 gtgacaaccc tgaatttttc gagcatttct tcaagaacgt caatggagaa ttcgcgagcc
     1321 atcgcacgga gggtgcgaat gttatttaaa ctttgtaaca ttacggacat aataaagaaa
     1381 accttttaac gccaaaacaa aaaagtattt cgtcgcgtag tgtaagcgat cacttattat
     1441 atttccacaa aatttaatct atttacagtc ctgcgcgaga ttatttcagc tgttattttc
     1501 tgaaactctc agaataattt caagattata tattagccat tttgatgagt aatttaggtt
     1561 attggtgaac agagtggcgc tctctgctcg ctaactccgc aagcctgaca aaaccgaagt
     1621 gaataaaaat gcaatataac agaataaaag aaaacacccc tcactggtgc tttctgctgt
     1681 taatgtacat tttctggcat caaaaatgct gtaaatgacc cggtcatgac aaacaccgaa
     1741 ttctctacgc ttatagcgtt acctcccccc caaactttca ttagtgaaat taaaggcaag
     1801 atcatatcta attgatttaa ttgaaaataa atcatattgg catgtaaaaa tccacaatgt
     1861 acaaaaaacg agccgttacg gaatatttta tctacaaaaa ctaactaaat aaaaattttt
     1921 cactaattga ttagtcatag acagcgatat acgctatgca aaaatgcaga tggcaatgag
     1981 atccactgct ttcatctcca ttaacatccc attacgcttt tattaaggag cattagcatg
     2041 ttctcaccgc agtcacgctt gcgtcatgca gttgcagata cgttcgcgat ggttgtttac
     2101 tgttctgtcg tgaacatgtg tattgaagtt ttcctctccg gaatgagctt cgaacagtct
     2161 ttttattcca gattggtagc gattccggtg aacatcttaa ttgcatggcc atacggtatg
     2221 taccgcgatc tgtttatgcg cgcggcacgc aaagttagcc cgtcgggctg gataaaaaat
     2281 ctggctgata tcctggctta tgtgacgttc cagtcaccgg tgtatgtggc gatcttgtta
     2341 gtggtgggcg cagactggca tcagattatg gcggcggtca gttcaaacat cgttgtttcg
     2401 atgttgatgg gggcggttta tggctacttc ctcgattatt gccgccgact gtttaaagtc
     2461 agccgttacc agcaggtaaa agcctgactc ttcttttcgc gactggcatc gccagtcgcg
     2521 tctttaactg gcttcgccaa ataaaccatt caaataacgt tcaagcgcaa cacgcgaact
     2581 aaagccatgc gggataccgt aatgctcgtg tcgctcgcct gctaaataga gcggaaattg
     2641 ctggtaatga tcgcaagttt taatatcgga agcgggttca gccagtgttg aactgcgttc
     2701 ttttaatgtt ggatgaatta ccagagcggt acgtcccatt cgcgcttcgc gattaacata
     2761 gacataattt tccccacggc ggtaaccata cgctttctgg gttaccacat cgacagtaaa
     2821 accgactttt tcaagtacgc gcgccacctc gtctggtcgt aaatacatat ttgatcctcg
     2881 ttattttcaa ccgcgggcct accttacctg attgcgcatt cacaacgctt tcagaaaagt
     2941 ccataaagcc gcgatgaacc tcagttaaga aaattatggt ctacactgaa aattacatcg
     3001 aattctaatg ggggatcata tgtttaaccg accgaaccgc aacgacgttg atgatggcgt
     3061 gcaggatatc cagaatgatg tcaatcaatt agctgacagc ctcgaatctg tattgaagtc
     3121 ctggggcagc gacgccaaag gggaagctga agccgcacgc agtaaagctc aggcgttgct
     3181 gaaagaaacc agggcgcgaa tgcatggtcg tactcgcgtc cagcaagccg cgcgcgatgc
     3241 cgttggctga gcggattctt ttgttcgtga aagaccctgg tgtagcgtgg gtacagcagc
     3301 tgcggtaggt atttttatcg gcgcactgtt aagcatgcgc aaatcgtagt gcaaaaatga
     3361 taataaatac gcgtctttga ccccgaagcc tgtcttcggg gtttcttttt gcctggtgaa
     3421 tcacaaaaat ccccctcccc cgtcacgctc atatccaggg taatttcgat cactatttgc
     3481 tatatattgt gtagttgaat cttttttcaa ctacatctag tatctctgta tcaacagaga
     3541 gacaacccga cgcgtatcat cgcgccgtat cttcatttta aacggaaata cgaatcatgc
     3601 gcataactat ttatactcgt aacgattgcg ttcagtgcca cgccaccaaa cgggcgatgg
     3661 aaaaccgggg ctttgatttt gaaatgatta atgtcgatcg cgttcctgaa gcagcggaag
     3721 cgttgcgtgc tcagggcttt cgtcagttgc cggtagtgat tgctggcgat cttagctggt
     3781 ctggtttccg tccggacatg attaaccgtc tgcatccagc gccacacgcg gccagtgcat
     3841 gagccagctc gtctacttct ccagcagctc cgaaaacacg cagcgtttta tcgaacgttt
     3901 aggtctgccc gcggtgcgca tcccgctcaa tgagcgggaa cggattcagg tagacgagcc
     3961 ttacatcctg atcgtgccct cttacggcgg cggcggtacg gctggcgcgg tgccacgaca
     4021 ggtaattcgc tttttaaacg acgagcacaa ccgggcgttg cttcgcggcg ttattgcttc
     4081 tggtaatcgc aactttggtg aggcgtatgg ccgcgccgga gatgtgattg cccggaaatg
     4141 cggcgtgccg tggctgtacc gttttgaact catgggtacg caaagcgata tcgaaaacgt
     4201 tcgtaaagga gtaaccgaat tttggcaacg acaaccgcag aatgcctgac gcaggaaacg
     4261 atggattacc acgcgctgaa tgcgatgctt aatctttacg atagcgcagg tcgcattcag
     4321 ttcgataaag accgccaggc cgttgacgcc tttattgcga cgcatgtgcg tccgaacagt
     4381 gtgaccttca gtagccagca gcagcgcctg aactggctgg tcaacgaagg ttactatgat
     4441 gaaagcgttc ttaatcgcta ctctcgcgac tttgtcatta cgctgtttgc ccacgcacac
     4501 accagcggtt ttcgtttcca gacattcctc ggggcatgga agttttacac cagctatacg
     4561 ttgaagacat tcgacggtaa acgttatctg gaagattttg ccgatcgagt cacgatggtg
     4621 gcgctgacgc tggcacaagg cgatgagacg ctggcgttgc aactgaccga tgaaatgctg
     4681 tcaggacgct ttcagccagc cacgccaaca ttcctcaact gcggtaagca gcagcgcggc
     4741 gaactggttt cctgtttttt gctgcgtatt gaagacaata tggagtcgat tggtcgggcg
     4801 gtaaattccg cactgcagct gtcgaaacgc ggcggcggcg tagcattttt gctgtcgaat
     4861 ctgcgggaag cgggcgcgcc aattaaacgt attgaaaatc aatcttctgg cgtaattccg
     4921 gtgatgaaaa tgttggaaga cgcattttcc tatgccaacc aactcggcgc gcgtcagggg
     4981 gcaggtgcag tctatttaca tgctcatcat cccgatattc tgcgttttct cgacacgaaa
     5041 cgggaaaatg ccgacgaaaa aatccgcatt aaaacactgt cgctgggcgt ggtgatcccg
     5101 gatatcactt tccatctggc aaaagagaat gcgcagatgg cgctgttttc gccctatgac
     5161 gttgagcgag tttatggcaa gccgtttgcc gatatcgcca ttagcgaaca ctatgacgaa
     5221 ctggttgccg atgaacgcat ccgcaaaaaa tacctcaacg cccgtgattt cttccagcga
     5281 ctggcagaaa tccagtttga gtccggctat ccctacatca tgtatgaaga cacagtaaac
     5341 cgtgctaacc ctatcgccgg gcgcataaat atgagtaatc tctgctcaga aattttgcag
     5401 gttaacagcg cctcagagta tgacgagaat ctcgactatg cccgcacagg ccatgatatt
     5461 tcctgcaatt taggttcgtt gaatattgcg cacaccatgg attcccccga tttttcccgc
     5521 acggtagaga ctgccgtgcg cggtttaacg gcggtgtcag atatgagtca tatccgcagc
     5581 gtgccgtcca tcgaagccgg aaatgccgcc tcgcacgcca tcggactggg gcagatgaat
     5641 ttacatggtt atctggcgag ggaaggcatc gcttatggtt cgccggaagc cctggatttc
     5701 accaatctct atttctatac catcacctgg cacgcactgc gtacctcgat gttgctggcg
     5761 cgcgaacgcg gtgaaacctt cgccgggttc aaacagtcac gctatgccag tggtgaatat
     5821 ttcagccaat atctgcaagg taactggcag ccgaaaacgg cgaaagttgg cgaactgttt
     5881 gcccgcagct gtattacgtt acctacccgt gagatgtggg cgcaactgcg cgacgacgtg
     5941 atgcgctacg gcatatacaa ccagaatctc caggcggtgc cgccaaccgg gtctatctct
     6001 tatatcaacc atgctacgtc gagtattcat ccgattgtgg cgaaagtaga gatacgcaaa
     6061 gagggcaaaa caggacgcgt ttactaccct gccccgttta tgactaacga aaatctggcg
     6121 ctgtatcagg acgcttacga aattggcgca gaaaagatca tcgacaccta cgcggaagcg
     6181 actcgccatg tcgatcaggg gctgtcgctg acgctgtttt tccccgatac cgccaccact
     6241 cgcgatatca acaaagcgca gatttacgcc tggcgcaaag gtatcaaaac gctctattac
     6301 atccgcctgc gtcagatggc gctggaaggc actgaaattg aaggctgcgt ctcctgtgca
     6361 ctttaaggaa tatctatgaa actctcacgt atcagcgcca tcaactggaa caagatatct
     6421 gacgataaag atctggaggt gtggaatcgc ctgaccagca atttctggct accagaaaag
     6481 gtgccgctgt cgaacgatat tcccgcctgg cagacattaa ctgtcgtaga acaacaactg
     6541 acgatgcgcg tttttactgg cctgacgctg ctcgacacgc tgcaaaatgt tatcggcgcg
     6601 ccttcgctga tgcccgatgc gctcacgcct catgaagagg cggtattatc gaatatcagc
     6661 tttatggaag cggttcatgc ccgctcttac agttcgattt tctcgacgct gtgccagacc
     6721 aaagatgtcg atgccgccta cgcctggagt gaagaaaacg caccgttgca gcgaaaagct
     6781 cagattattc agcaacatta tcgcggtgat gatccgctga aaaagaaaat cgccagtgtg
     6841 tttcttgaat cttttttgtt ctattccggt ttctggctgc cgatgtattt ttccagccgc
     6901 ggaaagctga ccaataccgc ggacctgatc cgtctgatta tccgcgatga agcagtccac
     6961 ggttactaca taggctataa atatcagaaa aacatggaaa agatatctct gggacaacgt
     7021 gaagagttga agagtttcgc cttcgatttg ttgctggaac tctacgacaa cgagttgcaa
     7081 tacaccgatg agctgtacgc cgaaaccccg tgggctgacg atgtgaaagc gtttctctgt
     7141 tacaacacca ataaggcttt gatgaatctg ggctacgaac cgttatttcc cgcagaaatg
     7201 gcggaagtga atccggcaat cctcgccgcg ctttcgccga atgccgatga aaatcacgat
     7261 ttcttttccg gttcaggctc ctcttatgtg atggggaaag cggttgaaac agaagatgaa
     7321 gactggaatt tctgagggtg ttattttcaa aaatatcact acccgcagca gggaaataat
     7381 tcccgccaaa tagcttttta tcacgcaaat attctgtagt gatctacact gatactctgt
     7441 tgcattattc gcctgaaact acaatattca ggcgtttttt cgctatctat gacaaaaaat
     7501 atcaactttt ctcgatttgc tctcagccct tatatcacgg aaaattccgg cgatttgctc
     7561 gcatcaatat tcatgccaca tttgccctca ggggttgcct cagattctca gtatgttagg
     7621 gtagaaaaaa gtgactattc ccattgggta atatatcgac ataaacaaat aaagaaatca
     7681 ttctattgca tggccattaa attaaaaatt aaaaatcttt ataaaatatt tggtgagcat
     7741 ccacagcgag cgttcaaata tatcgaacaa ggactttcaa aagaacaaat tctggaaaaa
     7801 actgggctat cgcttggcgt aaaagacgcc agtctggcca ttgaagaagg cgagatattt
     7861 gtcatcatgg gattatccgg ctcgggtaaa tccacaatgg tacgccttct caatcgcctg
     7921 attgaaccca cccgcgggca agtgctgatt gatggtgtgg atattgccaa aatatccgac
     7981 gccgaactcc gtgaggtgcg cagaaaaaag attgcgatgg tcttccagtc ctttgcctta
     8041 atgccgcata tgaccgtgct ggacaatact gcgttcggta tggaattggc cggaattaat
     8101 gccgaagaac gccgggaaaa agcccttgat gcactgcgtc aggtcgggct ggaaaattat
     8161 gcccacagct acccggatga actctctggc gggatgcgtc aacgtgtggg attagcccgc
     8221 gcgttagcga ttaatccgga tatattatta atggacgaag ccttctcggc gctcgatcca
     8281 ttaattcgca ccgagatgca ggatgagctg gtaaaattac aggcgaaaca tcagcgcacc
     8341 attgtcttta tttcccacga tcttgatgaa gccatgcgta ttggcgaccg aattgccatt
     8401 atgcaaaatg gtgaagtggt acaggtcggc acaccggatg aaattctcaa taatccggcg
     8461 aatgattatg tccgtacctt cttccgtggc gttgatatta gtcaggtgtt cagtgcgaaa
     8521 gatattgccc gccggacacc gaatggctta attcgtaaaa ctcctggctt cggcccacgt
     8581 tcggcactga aattattgca ggatgaagat cgcgaatatg gctacgttat cgaacgcggt
     8641 aataagtttg tcggcgcagt ctccatcgat tcgcttaaaa ccgcgttaac gcagcagcaa
     8701 ggtcttgatg cggcgctgat tgatgcgccg ttagcagtcg atgcacaaac gcctcttagc
     8761 gagttgctct ctcatgtcgg acaggcaccc tgtgcggtgc ccgtggtcga cgaggaccaa
     8821 cagtatgtcg gcatcatttc gaaaggaatg ctgctgcgcg ctttagatcg tgagggggta
     8881 aataatggct gatcaaaata atccgtggga taccacgcca gcggcggaca gtgccgcgca
     8941 atccgcagac gcctggggta caccgacgac tgcaccggct gacggcggtg gtgctgactg
     9001 gctgaccagt acgcctgcgc caaacgtcga gcattttaat attctcgatc cgttccataa
     9061 aacgctgatc ccgctcgaca gttgggtcac tgaagggatc gactgggttg ttacccattt
     9121 ccgtcccgtc ttccagggcg tgcgcgttcc ggttgattat atcctcaacg gtttccagca
     9181 attgctgctg ggtatgccag cgccagtggc gattatcgtt ttcgctctca tcacctggca
     9241 gatttccggg gtcggaatgg gcgtggcgac gctggtctcg ctgattgcca tcggcgcaat
     9301 cggtgcctgg tcgcaggcaa tggtgactct ggcgctggtg ttaactgccc tgctgttctg
     9361 tatcgtcatc ggtttgccgt tggggatatg gctggcgaga agtccgcgag cggcgaaaat
     9421 tattcgtcca ctgcttgatg ccatgcagac cacgccagcg tttgtttatc tggtgccaat
     9481 cgtcatgcta tttggtatcg gtaacgtgcc gggcgtggtg gtgacgatca tctttgcgct
     9541 gccgccgatt atccgtctga ccattctggg gattaaccag gttccggcgg atctgattga
     9601 agcctcgcgc tcattcggtg ccagcccgcg ccagatgctg ttcaaagttc agttaccgct
     9661 ggcgatgccg accattatgg cgggcgttaa ccagacgctg atgctggccc tttctatggt
     9721 ggtcattgcc tcgatgattg ccgtcggcgg gctgggtcag atggtacttc gcggtatcgg
     9781 tcgtctggat atggggcttg ccaccgttgg cggcgtcggg attgtgatcc tcgccattat
     9841 cctcgatcgc ctgacgcagg ccgttgggcg cgactcacgc agtcgcggca accgtcgctg
     9901 gtacaccact ggccctgttg gtctgctgac ccgcccattc attaagtaac tctgcacttg
     9961 cccggtgacg ccgggcatta tcaccctgcc aaaaaaagga ataacaatgc gacatagcgt
    10021 actttttgcg acagcgtttg ccacgcttat ctctacacaa acttttgctg ccgatctgcc
    10081 gggcaaaggc attactgtta atccagttca gagcaccatc actgaagaaa ccttccagac
    10141 gctgctggtc agtcgtgcgc tggagaaatt aggttatacc gtcaacaaac ccagcgaagt
    10201 ggattacaac gttggctaca cctcgcttgc ttccggcgat gcaaccttca ccgccgtgaa
    10261 ctggacgcca ctccatgaca acatgtacga agctgccggt ggcgataaga aattttatcg
    10321 tgaaggggta tttgttaacg gcgcagcaca gggttacctg atcgataaga aaaccgccga
    10381 ccaatacaaa atcaccaaca tcgcacaact gaaagatccg aagatcgcca aactgttcga
    10441 taccaacggc gacggtaaag cggatttaac cggttgtaac cctggctggg gctgcgaagg
    10501 tgcgatcaac caccagcttg ccgcgtatga actgaccaac accgtgacgc ataatcaggg
    10561 gaactacgca gcgatgatgg ccgacaccat cagtcgctac aaagagggca aaccggtgtt
    10621 ttattacacc tggacgccgt actgggtgag taatgagctg aagccaggga aagatgtggt
    10681 ctggttgcag gtgccgttct ccgcactgcc gggcgataaa aatgccgata ccaaactgcc
    10741 gaatggcgcg aattacggct tcccggtcag caccatgcat atcgttgcca acaaagcctg
    10801 gaccgagaaa aatccggcgg cagccaaact gtttgccatt atgcagttgc cagtggccga
    10861 tattaacgcc cagaacgcca ttatgcatga cggcaaagcc tcagaaggcg atattcaggg
    10921 ccacgttgat ggctggatca aagcccacca gcagcagttc gatggctggg tgaatgaggc
    10981 gctggcagcg cagaagtaat ttttattcgg gcggataagg cgttttcgcc tcatccgccg
    11041 ctctgtacac aatgcctgat gcgacgctga cgcgtcctat catgcctaca aaccacatcg
    11101 cacatttcaa caagctatct ttcatcccat attcatcaac atccgctatt attgatttcc
    11161 agcttaatca tcacctgatg aacaaaaata atgactaaac ctaatcatga gcttagcccg
    11221 gcgctgatcg tgctgatgtc tatcgccacc ggtctggcgg tagccagtaa ctattacgcc
    11281 cagccattgc tcgacaccat cgcgcgtaac ttttcccttt ccgccagttc ggcaggcttt
    11341 attgttaccg ccgcgcagtt gggctatgcc gcaggtctac tgtttcttgt tcccctcggt
    11401 gatatgtttg aacgccgccg cctgattgtc tcgatgacct tactggcagc gggcggtatg
    11461 ttaatcactg ccagcagtca gtcactggcg atgatgatcc tcggtacggc attaaccggt
    11521 ttattctcag tcgtggcaca aattctggtt ccgctggcag cgacgctggc ttcaccggat
    11581 aaacgcggca aagtggttgg caccattatg agcggtctgc tgttggggat attgctggca
    11641 cgaaccgttg ccgggttact ggcaaacctc ggcggctggc gcaccgtctt ttgggttgct
    11701 tcggtgttaa tggcactgat ggcgctggca ttatggcgtg gtctgccaca aatgaaatca
    11761 gaaacccacc tcaactaccc acagttgttg ggttccgttt tcagcatgtt catcagcgat
    11821 aagatcctgc gcacccgcgc gttgctgggc tgcatgacct ttgccaactt cagcattctc
    11881 tggacctcaa tggccttttt gcttgccgct ccacctttta actacagcga tggtgacatt
    11941 ggtctgtttg ggcttgcggg agctgccgga gcgttgggcg ctcgtccggc gggcggtttt
    12001 gccgacaagg gcaaatcgca ccacaacttt cggtctgctt ctgctgttac tttcatggct
    12061 ggcaatctgg tttggtcaca cttccgtact ggcgttgatt atcggcattc tggtgctgga
    12121 cctcaccgta cagggcgtgc atatcactaa ccagacggta atttatcgaa tacatcctga
    12181 tgcgcgtaat cgcctgaccg caggttacat gaccagctac tttattggcg gtgccgccgg
    12241 ttcgctaatt tcagcctcag cctggcaaca tggcggttgg gctggcgttt gtctggctgg
    12301 cgcgacgatt gccctggtta acttactggt ctggtggcga ggttttcatc gtcaggaagc
    12361 cgcaaattaa gcaatcagca accgttttcg tggctttaca cttataaggg tgttaagaag
    12421 cccatcagtc tgataaggtt aagatattca ttcagtctat ttataatatt aacaatcgtt
    12481 aagcgtacac tctatggaaa gccctactcc acagcctgct cctggttcgg cgaccttcat
    12541 ggaaggatgc aaagacagtt taccgattgt tattagttat attccggtgg cctttgcgtt
    12601 cggtctgaat gcgacccgtc tgggattctc tcctctcgaa agcgtttttt tctcctgcat
    12661 catttatgca ggcgcgagcc agttcgtcat taccgcgatg ctggcagccg ggagtagttt
    12721 gtgggttgct gcactgagcg tcatggcaat ggatgttcgc catgttttgt atggcccgtc
    12781 actgcgtagc cgtattattc agcgtctgca aaaatcgaaa accgccctat gggcgtttgg
    12841 cctgacggat gaggtttttg ccgccgccac cgcaaaactg gtacgcaata atcgccgctg
    12901 gagcgagaac tggatgatcg gcattgcctt cagttcatgg tcatcgtggg tatttggtac
    12961 ggtaataggg gcattctccg gcagcggctt gctgcaaggt tatcccgccg ttgaagctgc
    13021 attaggtttt atgcttccgg cactctttat gagtttcctg ctcgcctctt tccagcgcaa
    13081 acaatctctt tgcgttaccg cagctttagt tggtgccctt gcaggcgtaa cgctattttc
    13141 tattcccgtc gccattctgg caggcattgt ctgtggctgc ctcactgcgt taatccaggc
    13201 attctggcaa ggagcgcccg atgagctatg aggttctgct gcttgggtta ctggttggcg
    13261 cggcgaatta ttgcttccgc tatttgccgc tgcgcctgcg tgtgggtaat gcccgcccaa
    13321 ccaaacgtgg cgcggtaggt attttgctcg acaccattgg catcgcctcg atatgcgctc
    13381 tgctggttgt ctctaccgca ccagaagtga tgcacgatac acgccgtttc gtgcccacgc
    13441 tggtcggctt cgcggtactg ggtgccagtt tctataaaac acgcagcatt atcatcccaa
    13501 cactgcttag tgcgctggcc tatgggctcg cctggaaagt gatggcgatt atataaccca
    13561 caagaatcat ttttctaaaa caatacattt actttatttg tcactgtcgt tactatatcg
    13621 gctgaaatta atgaggtcat acccaaatgg atagttcgtt tacgcccatt gaacaaatgc
    13681 taaaatttcg cgccagccgc cacgaagatt ttccttatca ggagatcctt ctgactcgtc
    13741 tttgcatgca catgcaaagc aagctgctgg agaaccgcaa taaaatgctg aaggctcagg
    13801 ggattaacga gacgttgttt atggcgttga ttacgctgga gtctcaggaa aaccacagta
    13861 ttcagccttc tgaattaagt tgtgctcttg gatcatcccg taccaacgcg acgcgtattg
    13921 ccgatgaact ggaaaaacgc ggttggatcg aacgtcgtaa aagcgataac gatcgccgct
    13981 gcctgcatct gcaattaacg gaaaaaggtc acgagttttt gcgcgaggtt ttaccaccgc
    14041 agcataactg cctgcatcaa ctctggtccg cgctcagcac aacagaaaaa gatcagctcg
    14101 agcaaatcac ccgcaaattg ctctcccgtc tcgaccagat ggaacaagac ggtgtggttc
    14161 tcgaagcgat gagctaacgc gtcatctcgc tcaaaaatcc agatttataa aagaaaaaat
    14221 gaccggccag catcgcaaca tgctggcctt tttggcaagc aggtcggctc agccgatgag
    14281 ttaagaagat cgtggagaac aatatgagcg caaatgcgga gactcaaacc ccgcagcaac
    14341 cggtaaagaa gagcggcaaa cgtaagcgtc tgctcctcct tctcaccttg ctctttataa
    14401 ttattgccgt agcgataggg atttattggt ttttggtact gcgtcatttc gaagaaaccg
    14461 atgacgcata cgtggcaggg aatcagattc aaattatgtc tcaggtgtct ggcagcgtga
    14521 cgaaagtctg ggccgataac accgattttg taaaagaagg cgatgttctg gtcactctcg
    14581 acccgacaga tgctcgccag gcgtttgaaa aagccaaaac tgcactggct tccagcgttc
    14641 gccaaaccca ccagctgatg attaacagca aacagttgca ggcgaatatt gaggtgcaaa
    14701 aaatcgccct cgcgaaagca caaagcgact acaaccgccg tgtgccgctg ggcaatgcca
    14761 acctgattgg tcgcgaagag ctgcaacacg cccgcgacgc cgtcaccagt gcccaggcgc
    14821 aactggacgt cgctattcaa caatacaatg ccaatcaggc gatgattctg gggactaaac
    14881 tggaagatca gcctgccgtg caacaggctg ccaccgaagt acgtaacgcc tagctggcgc
    14941 tggagcgtac ccgtattgtc agtccgatga ccggttatgt ttcccgtcgt gccgtgcaac
    15001 cgggtgcgca aattagccca acgacgccgc tgatggcggt cgttccagcc accaatatgt
    15061 gggtggatgc caactttaaa gagacgcaga ttgccaatat gcgcatcggt cagccggtca
    15121 ccatcaccac cgatatttac ggcgatgatg tgaaatacac cggtaaagtg gttggtctgg
    15181 atatgggcac aggtagcgcg ttctcactgc ttccggcgca aaatgcgacc ggtaactgga
    15241 tcaaagtcgt tcagcgtcta cctgtgcgta tcgaactgga ccagaaacag ctggagcaat
    15301 acccgctgcg cattggtttg tccacgctgg tgagcgtcaa taccactaac cgtgacggtc
    15361 aggtactggc aaataaagta cgttcaacac cggtcgcggt aagtaccgcg cgtgaaatca
    15421 gcctggcacc tgtcaataaa ctgatcgacg atatcgtaaa agctaacgct ggctaatcca
    15481 gaggtgcgtg tgatgcaaca gcaaaaaccg ctggaaggcg cgcaactggt cattatgacg
    15541 attgcgctgt cactggcgac attcatgcag gtgctgaact ccaccattgc taacgtggcg
    15601 atccccacta tcgccgggaa tctgggctca tcgctcagcc aaggaacgtg ggtaatcact
    15661 tctttcgggg tggcgaatgc catctcgatc ccgcttaccg gctggctggc aaagcgcgtc
    15721 ggggaagtga aactgttcct ttggtccacc atcgcctttg ctattgcgtc ttgggcgtgt
    15781 ggtgtctcca gcagcctgaa tatgctgatc ttcttccgcg tgattcaggg gattgtcgcc
    15841 gggccgttga tcccgctttc gcaaagtcta ttgctgaata actacccgcc agccaaacgc
    15901 tcgatcgcgc tggcgttgtg gtcgatgacg gtgattgtcg cgccaatttg cggcccgatc
    15961 ctcggcggtt atatcagcga taattaccac tggggctgga tattcttcat caacgtgccg
    16021 attggcgtgg cggtggtgtt gatgacactg caaaccctgc gcggacgtga aacccgcacc
    16081 gaacggcggc ggattgatgc cgtggggctg gcactgctgg ttattggtat cggcagcctg
    16141 cagattatgc tcgaccgcgg taaagagctg gactgttttt catcacagga aattatcatt
    16201 cttaccgtgg tggcggtggt ggctatctgc ttcctgattg tctgggagct gaccgacgat
    16261 aacccgatag tcgatctgtc gttgtttaag tcgcgcaact tcaccatcgg ctgcttgtgt
    16321 atcagcctcg cgtatatgct ctacttcggc gctattgttc tgctgccgca gttgttgcag
    16381 gaggtctacg gttacacggc gacctgggca ggtttggcct ctgcgccggt agggattatt
    16441 ccggtgattc tgtcgccgat tatcggccgc ttcgcgcata aactggatat gcggcggctg
    16501 gtaaccttca gctttattat gtatgccgtc tgcttctact ggcgtgccta tacctttgaa
    16561 ccaggtatgg atttttgcgc gtcggcctgg ccgcagttta tccaggggtt tgcggtggtc
    16621 tgcttcttta tgccgctgac caccattacg ctgtctggtt tgccaccgga acgactggcg
    16681 gcggcatcga gcctctctaa ctttacgcga acgctggcgg ggtctatcgg cacgtcgata
    16741 accacgacca tgtggaccaa ccgcgagtcg atgcaccatg cgcagttgac tgagtcggta
    16801 aacccgttca acccgaatgc ccaggcgatg tacagtcaac tggaagggct tgggatgacg
    16861 caacagcagg catcaggctg gattgcccag cagatcacca atcaggggct gattatttcc
    16921 gccaatgaga tcttctggat gtcagccggg atattcctcg tactgctggg gctggtgtgg
    16981 tttgctaaac cgccatttgg cgcaggtggc ggcggaggcg gtgcgcacta agtacaacta
    17041 agccagttca tttgaactgg cttttttcaa ttaattgtga agatagttta ctgactagat
    17101 gtgcagttcc tgcaacttct ctttcggcag tgccagttct tcgttgctgt tgatgcgtac
    17161 ctcacgttcc agaatgctac gcgcaatatc ctgcgcttcc tgcaacgagt gcatctggta
    17221 agtgccacac tggtagacgt tcagttccgg gatctgattc tgatcctgca ctttcagcac
    17281 gtcttccatt gccgctttcc aggcatcagc aacacgctgc tcatctggcg taccaatcag
    17341 actcatataa aaaccggtgc ggcagcccat tggcgagata tcgataatct ctacaccatt
    17401 accgttaaga tggttacgca taaaaccagc aaacaggtgc tccagggtat ggatccctct
    17461 ttctggcatc acttctttgt tcggcacgca gaagcgcaga tcgaacacgg tgattgcgtc
    17521 gccatgcggg gtgttcattg ttttcgccac ccgaactgca ggcgcttcca tccgggtatg
    17581 atcgactgtg aagctatcta acaacggcat ttagccacct ccggtaattt ttttaaaaat
    17641 tttctgaact ctttcttccc aggcgagtct gagtatatga aagacgcgca tttgttatca
    17701 tcatccctga attcagagat gaaattttgg ccactcccga gtggcctttt tcttttctgt
    17761 caggcgtgtt tttccagcca caccgcaaac ggttcggtat cagcggtttc catttcctgc
    17821 tgacggcgtt cagatgcctc gcgctcggct acaaaatcct cttcgcgcag aatttccagc
    17881 ggctcttcac gcagcagatt acggtaggct tctgcaaacg ctttgcctgt tccgccaata
    17941 ccagtatcaa tcatagacct taagatacgg gcagagaaag tcagatcggg attatcgaag
    18001 caggcaacca gttcatcaca cactttctga tacgcttcgc cgccgttaat gctatccagc
    18061 gtttgcgcga cgcgtttcag atcgcggaac agatctttac ccacctgcag taacgggaac
    18121 tgcgcggttt cgcagccgat acccagcgtc aggcccggtt tgcgaccttc aaggatcacc
    18181 cggttccagt taacgcgcgt acaggcaagt tcgctactgc tcatttccgg tgcatcagcc
    18241 agcgcacacc agaccataaa caggtcgagg aatcgcacct gctgttcatc tacaccaatc
    18301 ggcgagaacg ggttgatgtc cagcgaacgc acttcaatat attcaatgcc gccacgtaac
    18361 agcgcatcag aaggtgactc gccgctgcgg gtaacgcgtt ttggacgaat cggcgcatac
    18421 agttcgtttt caatctgcag cacgttgcta ttgatttgca gcctcttacc gtctttctca
    18481 ataccaatct tcgcgtactc ttccgatggc gttttgattg cctgtttaag gcccgctacg
    18541 tactcgtaaa gatcgttgaa ggtaatacca agattgcttt gcgatttatt ggtatagccg
    18601 agatcgctca aacgaagaga ggtcgcatac ggcaggtaat acataccgca ctcggttttc
    18661 tcaaacggca gcgacgttgg ttttccttgc aggaaagaag aacaaatcgc cggagatgca
    18721 ccaaacagat aaggaataac ccaaccgaaa cgatagtaat tgcggataac gcggaaatag
    18781 cccgcagaaa ttttctcttt ggcatcagcg cccgagatat caccgcactt cgcttgccag
    18841 aatgccattg gcaaagagaa attgtagtgc acgccggaaa tggtttgcat cagcgcgccg
    18901 tagcgatttt tcagcccttc acgatacagc gttttaaagc gtccggtgtt agaagtgccg
    18961 tactgtgcca gttcgatgtc ctgaccttct gcgatgtagc atggcatact taacggccac
    19021 atccgctcat cgcccatatt gcgcgccgta taacgatgca gatcgcgcat aaaggtcagc
    19081 atatgttcaa tatcaccatc cactggtgta atgaattcca gcaatgcttc cgcaaaatcg
    19141 gtagtaatcc atttgtgcgt cagtgcggaa cctaatgctt caggatgacc tgttgttgcc
    19201 agtgtgccat cagcattaac acgcaaagtt tcgcgctcca gcccacgctg tatccccttt
    19261 aacgcctgag gatgtttttc cagccaggcc agcgcctgtg atacgtccgg gatcaaattg
    19321 acctcccgcc tgtcaaaatc gttttaatta gcataactgt aatggtgacc atatgtgcag
    19381 gcctacaatt agtgccacca catcatgccc tgaacggtcg ctgctgcaac tgcaacatag
    19441 cgtaacgctt taccaaggca caaaaaaaag attactggcc cccaggagat gcgcatccat
    19501 cccgctaaca gacacagtaa atcgccaacc acgggcatcc agcttaataa tagcgtgact
    19561 gcaccatagc gtttcagcca gccggtagct ttctcttgcc agcgcgatgt tttacgcaat
    19621 ggaaagaaac gcccaaggat aacgttagtt aaccctccaa ggctattacc cattgttgct
    19681 gttaaaacta aaacccaggg atgactgatc ccggaaagca acattgccac cagcacgact
    19741 tcggagttgc cgggtaatag tgtagcgctg agaaaactac tggcaaacaa cgagaaaagc
    19801 gataacgctt cactcacagc aagcgaacat ccacagcgtc catgcctgct gcacgggccg
    19861 cctgaatacc gaaatcggca tcttcaaaga ccacacactg cgtcggttgc acgcccatac
    19921 gctgcgcgca caacaaaaat gtgtctggcg cgggtttatg gtgtttgacg tgatcagcag
    19981 cgacgacggc gtcaaaataa cggcgtaatc ccaggtgcgc cagcaatgcc tcagcgatgg
    20041 cgctttcact ccccgttcct acagccattg ggcgacgacc atgccaactt ttcaccactt
    20101 caacaagagg aagcggctcg acactatcca gcagcatact tcttactgct tctgtttttt
    20161 cacgcgctaa cgcatgcggg tcgagatcgg cctgattcag ctcaataatt gcctgagcaa
    20221 tacgccaggt gggcgatcca ttaagcgcaa tcatcgcctg aatatcgtac tgaagaccgt
    20281 agtgccctaa tacttcgcgc cacgctttac ggtgcgtagg cgccgtatcc aggattgtgc
    20341 catccatatc aaaaattaaa cctgcataac gctcgtacat ggtcttctcg caagtcgaaa
    20401 aatatgacgc tactttagcg taattgcttg attttgtcgc tgatggcgaa ggagaagaaa
    20461 attcaggaaa tggataaagt agttatttag aaatgagata tttttgaagg aaattttttg
    20521 gagaatatgg tgcatccggg aggattcgaa cctccgaccg ctcggttcgt agccgagtac
    20581 tctatccagc tgagctacgg at
//
LOCUS       DAJTQQ010000068        20477 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000068 DAJTQQ010000000
VERSION     DAJTQQ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.068"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..4311)
                     /gene="mukB"
                     /locus_tag="OO309_002715"
     CDS             complement(<1..4311)
                     /gene="mukB"
                     /locus_tag="OO309_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR5847808.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     N"
     gene            complement(4311..5015)
                     /gene="mukE"
                     /locus_tag="OO309_002716"
     CDS             complement(4311..5015)
                     /gene="mukE"
                     /locus_tag="OO309_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR5847809.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(4996..6318)
                     /gene="mukF"
                     /locus_tag="OO309_002717"
     CDS             complement(4996..6318)
                     /gene="mukF"
                     /locus_tag="OO309_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR5847810.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6315..7100)
                     /gene="cmoM"
                     /locus_tag="OO309_002718"
     CDS             complement(6315..7100)
                     /gene="cmoM"
                     /locus_tag="OO309_002718"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR5847811.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7236..8015
                     /gene="elyC"
                     /locus_tag="OO309_002719"
     CDS             7236..8015
                     /gene="elyC"
                     /locus_tag="OO309_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR5847812.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(7992..8885)
                     /locus_tag="OO309_002720"
     CDS             complement(7992..8885)
                     /locus_tag="OO309_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR5847813.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9039..9785)
                     /gene="kdsB"
                     /locus_tag="OO309_002721"
     CDS             complement(9039..9785)
                     /gene="kdsB"
                     /locus_tag="OO309_002721"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR5847814.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(9782..9964)
                     /gene="ycaR"
                     /locus_tag="OO309_002722"
     CDS             complement(9782..9964)
                     /gene="ycaR"
                     /locus_tag="OO309_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR5847815.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10016..11248)
                     /locus_tag="OO309_002723"
     CDS             complement(10016..11248)
                     /locus_tag="OO309_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR5847816.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11285..12271)
                     /gene="lpxK"
                     /locus_tag="OO309_002724"
     CDS             complement(11285..12271)
                     /gene="lpxK"
                     /locus_tag="OO309_002724"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR5847817.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHEDVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12268..14016)
                     /gene="msbA"
                     /locus_tag="OO309_002725"
     CDS             complement(12268..14016)
                     /gene="msbA"
                     /locus_tag="OO309_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR5847818.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14053..16312)
                     /locus_tag="OO309_002726"
                     /pseudo
     CDS             complement(14053..16312)
                     /locus_tag="OO309_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16519..16803)
                     /gene="ihfB"
                     /locus_tag="OO309_002727"
     CDS             complement(16519..16803)
                     /gene="ihfB"
                     /locus_tag="OO309_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR5847819.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(16963..18636)
                     /gene="rpsA"
                     /locus_tag="OO309_002728"
     CDS             complement(16963..18636)
                     /gene="rpsA"
                     /locus_tag="OO309_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR5847820.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18747..19430)
                     /gene="cmk"
                     /locus_tag="OO309_002729"
     CDS             complement(18747..19430)
                     /gene="cmk"
                     /locus_tag="OO309_002729"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR5847821.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19603..20367)
                     /gene="ycaL"
                     /locus_tag="OO309_002730"
     CDS             complement(19603..20367)
                     /gene="ycaL"
                     /locus_tag="OO309_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR5847822.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4929 a         5676 c         4967 g         4905 t
ORIGIN      
        1 attttccggc gctgcgatga tgagctgcat ttgcaaacgt tcacacaatt caaacagcgt
       61 ggcgatagaa cgagcatcca gtcgcgctgc ttcatcgagg aacagcaggc ggcaaggaga
      121 gatatcttta ccgcgcaggc ggcgagattc atcttcccag ctttgtacca ccatcaccag
      181 aatcgacata ccggtaccaa tcgcctcacc ggtcgacaat gcaccagact ctgcgcgcaa
      241 ccaaccatcg gaaccacggt taacctcaac ttccatttcc agatagttgc ggtaatccag
      301 cagttcttcg ccaatggtct gcggcgtgcg ctgccccata tcaatctgcg gattaagacg
      361 ctgatacaat ttcgccaacg cttccgagaa ggtcagacgg ttgctgttaa acagatcctg
      421 atgctgctcg tgctgttcag agagcacatc cagcagcatg gcgtgcgttt cacgcacgtt
      481 cacgttgaga cgcacgctgt tcacctgacc aaacgatacg ttctgcaacc cctggttgag
      541 catacggata cggttctgct cgcgctgaat ggttttgcga atgatgttcg ccacgctgcg
      601 ggaactgatc gccagtttct gttcacggga ggttaattct tcggtcagac ggctaagttc
      661 aatctccatc tgttcgatag cttccaccgg atcatcggta cgaataatat cctgacgaat
      721 acgttcacgc agatgctgat aaaccgccac gaagaactga attttacgct ccggacgttt
      781 cggatcttcc gacatgcgca gcacgtcgcg cagatgttcg ttatccgcca ccgccagacg
      841 cagcgcacct aacgccttat ccgacatgga acgcaaatca tcagcggaga gataagccag
      901 ctcacgacgg tgtaagcggc gctcaacgcc gttatctttc accatgcgca tcaccgcaca
      961 ccagcccgct ttggcggtca ctacctgctc gcgcatctca aagtaatccc gctccagctt
     1021 gcgcagtttg cgggtcaggt tgtccatctc cgcttcgcag aaggtaagcg ctttttccag
     1081 ttgattgcgg cgtgaacggt tattgctcag ttgcgcatgc agctcgtcac ggcgaatacg
     1141 cgcccgctct tctgccccgc tatcagcacg cacgccgata tcctgcaatt cacgttgcag
     1201 atcgttgagt agctcttttt tggtgtcgta agaacttttc agcgaagcca gcacctggtt
     1261 gtactgactc aactgcgctg cgtgaccgcg caacgcttcg cgagcgcggg tacgctccgc
     1321 ttccgcctgt tccagacgtt cacgcagttt ttcgttgaga tcgctgttac cgctaagcat
     1381 ttctgccgag tcagaatagc taaagtgcgc acgacgctgc accacttccg tcagggcaaa
     1441 cgcttgctga cgggcatcgc gctgcatctg ctgagagtac gcgtaatctt cttttaactg
     1501 ttcgaactgt tccgggtcgc tctgcaatac cgaaacgatc ggttccagtt tcgccagttg
     1561 attgccaaac tgctgaacaa aacgcgcggc ttcctgggct tcatccagac gttcgcggat
     1621 ttcatcgacg cgatccgcca ggctgtcatc agccaacagg ttgagacgcg gcagaatgcg
     1681 gttcagcgcc gtaacgcctt ctttcgcctg ctcaaactga atacgctgct gctggttatc
     1741 attttcatga ttactcaacg cccgctccag ttcgactcga cggctgttca gttgacggat
     1801 ttctgcttcc gggtcagact caaacgccac cgccagatga ctgccgataa agcggctgaa
     1861 cgcctgatgc agacgctggg ttttctgtac atcaaaggag agcgtggcga agcgttcgga
     1921 aagcacttca cgctcggcat ggaggctttc aatacggctt tcacgcgcag cacgaccaaa
     1981 cagcggcact tccgggaaac gtgaataacg ccactgtcga tcggcgattt tcaccaccac
     2041 cgctttttcc agctcatcaa cgctgaacac gctatcatcg aatgactgcg gatccccttc
     2101 gatcaaatag agatcttccg ggcaatcggt caagccttcc aggtgttcgg ttacctgtga
     2161 cagatctggc accacgatgg cgtggcgtga cgggccatac agagccgaga agtacggcgc
     2221 atcttccagg ctaacgtcgt cataaatttc tgacagcagc acaccaccaa aacgctccgc
     2281 cagcgcgttc agacgctgat cttcagagcc gccaggctgg cttaaacgtt cgatctcttc
     2341 atcgacggcg tttttgcgcg cgcctacttc atcgcgttca acaatcgcct cgcgctcacg
     2401 ctccagcaac tgttgcagat attcagtgac gtcctggctg gaggagaact cttcgccgca
     2461 ctgttcactc aactggttga gactgttttg cgctgccagc caaaccggtg cacgctgcat
     2521 caaactctga atgcgagact gcagctgttc ctgttcctgc cgcagtgcca tccgctcttc
     2581 acgggcgtta gatacgctat cggaaagaga ggcaatgcgt gcttccagct cctgatgcag
     2641 agcttccagt tcatcaatat caaaattctt gccctgacgt ttgcagaaat ctgccagcag
     2701 acgctcagct tcttgctgct cacgcagacg ctgttccagt tcgcttaagc gcatccgcaa
     2761 cggctgaacc tgctctgcca ggtgacgctg atcaacccct tcgcgcaata gttcgcgagc
     2821 gacatcccac gcctcgttac gcgccagcgg gccgttgatt gccaccacca gctgataagc
     2881 ctgctcaaac tggctgtgcg cggtttgcgc catgctcatt ttctgctcaa gagagagcat
     2941 tttttcagtc gcttccagct ctttcgcctg gaaggtttcc agccattcgg cggcgctgtc
     3001 ggcggttaag tccggcagat ggcacagttc tttggcacga ttaagcgcag caatcgcctg
     3061 gttatactgg atcgcgcgcg tttgctggac gtccagcgcc tgctggtagt cagcaagctg
     3121 gcttttcagc tcgtccactt ccagttcggc agcttccgcg cgagcctcat tctcttcctg
     3181 gcgttcgatg gcttctgcca ccacttcatt ttgctcttcc agacggatct gcagctcatc
     3241 gagatccact tcgtagcgtt cgatcttctc ctgctgacgc aatgcggttt gcaccaggtt
     3301 caggtgatcg ctggccgcct gataatccgc ttccagatca ccttcggcac cgttgtgctc
     3361 tgccagctca cgcgccatat cgacgtgttt gtactgctca gccgccagtt gctgacgcga
     3421 agtatgtagc tcgcgacgaa actccagggc tttgtcgaga tggacacggc gctcgttggc
     3481 gtgacgcatg tagtccgccg ccacgtagtt ggtagcttcg ctgatcagat gcttaaacag
     3541 gtcacggtcc gactgggtga cacgaatcgc ttccagcgtc atgcggtttt cacgcagcgc
     3601 cgcttccatg tcctggaacg ctttacgcac gccgctgttt tccggcaaca ggtagtcgcg
     3661 cagagaacgg gtaatggcac tggagatccc gccatacagc gaagcttcga tcagacgata
     3721 gaatttgcta cggtcagatg cggagcgcag acgacgcgcg atgatgccca gatcgaacat
     3781 cagcgagtgg taatcggtaa tggagttgaa ctgtttaaac tgcacgcctt ccatcgcctc
     3841 gagcttgtct ttcagctcgt taagcggcag cacgcgcgcc tggcgttcgt tcagggtttc
     3901 ggtcaccagc tgtgtcggct gcaccgacat cggcagtccc tgaatggcaa atggtttgat
     3961 atccacttta cgatcgcgtc cggcaacctg ttgcagacgc acaccgacca ccacgcgctg
     4021 gtggcgcgag ttaatggtgt cgagcatcga ataacagaca cccgctttca gcttaccgtg
     4081 cagaccttta tcgcgcgaac cgctggtggc cccggcttcc gtagtgttac ggaaatgcag
     4141 cagggtcagg tcggggatca gcgccgtaac gaacgccgcc atcgtggtgg atttacccgc
     4201 cccgttaccg ccggaaagcg tcgtgaccag ctcgtcaagg tcaaaagttc gggcaaaaaa
     4261 gccgttccag ttaatcagcg tcagtgagcg aaatttaccg cgttcaatca ttattcttcc
     4321 tctccgctat ctggctgact ctcttcggtt tcatcgttga gttgcagatg attttcaatc
     4381 ggcattgctt cgccatcacg aatcaggcga cgctgtgctt cacggggatc gtcgccagca
     4441 cgcacatcgg ctccgaagcg gaacaccgat tcggtaatgc ggaacttgct gctgtcgtgg
     4501 cccataaacc acaccatgcc taaacgacgc agacggttga gcgaagaacg tactttctcc
     4561 tgcaacttct gacggtcaac gtctgaaccg gttgaacggt tgttcaccag tttcagcagt
     4621 ttcgcttcat cggccagggt gagcagttcg tcgtacagtt cctgctgggt gaaaatcccc
     4681 tcattcgcca gccgttccgg gctgagatag agataacaga ggattttccc gaccatcata
     4741 tccagttccg acaagacgga acgcgggata agagtggtgg aacgtgggcg taaatagaag
     4801 aacccttccg gtgcgcgaat aagctcaacg ttataacgcg cgtaaaactc ttccagatat
     4861 tcctgaaaat ccatcaggaa tgcatgatta tccagttcgt cgaggccaat atggcggcct
     4921 gaacgtaagg cgctgtccag cgccggaaat aacggattcg ccagcgcctg cgccagctta
     4981 accggcatca cttgttcaat atttgtcgat gacatgcgcc tgtaccttgg ctccgtaatc
     5041 attaatcggc tgccatttcg ctggcagtcc ggtgaaatct gcttgcgcta cgccaagacg
     5101 taccgcctga tcaataacaa tacgcgcaac gtcaaagtga cgtgcacgcg gatactgtga
     5161 cagatattcg cgtaccacca gaccaagatc cagcggcact tgtctggttt tgtacacggc
     5221 aagttgttct tcgatgatcg ccgccagctg ttcgcggatc tcgttaaact cttcgtattc
     5281 cagatcctca ggaagttccc cagtcacttc ttcatcgcgc agtgccatct cttcgtcacg
     5341 catatccagc agacgatcgg cattggcata agttagcgcc cacggctcat caaaataggt
     5401 ttgtaccgac tgacgtaacc gctgagcaaa gacgcggttt ttatccatat cgatcgcggt
     5461 acgaataaat ttgtgtacgt ggcggtcgta gccaatccac aagtcgatgg attgctggcc
     5521 ccaactgata atacgatcga gtttgctctg cagatcgaac accagacgat cgacgaaatg
     5581 cagatcgtca tgggtcatcg tcgcatcctg aatgcgcaac agattagcct gcaatttgtc
     5641 gcctgccgct tccagcgtat cctgcaattc acgcagcgtt ccggaagttt ccgaaagcaa
     5701 caattcacag ctggaaatcg ccgcccgcca gtctttgttc agcaactggg cgatatcgtc
     5761 cttcacctgc tgctgctgtt cgtccatcag acgttgcgtc aggtcgatac tgtcgaaaat
     5821 ttctgctacc gaatatttca gtggcgcata gacattacgg tgccagtgaa attcatcacc
     5881 gccctcttcg gcggcatctg ctgcgcgttt gagctcaccc gccacaatcg acaactgcat
     5941 agaaagacgc agcgtagaaa actcgcgctg acggatgtag tagtcagtaa tgccgatgcc
     6001 gagcggcgtc agacggtaaa ttgcgttccc ttccgcctgc tcgctggtaa agcggttcag
     6061 cagacgttga cgcaccatgt cgttgatcgc gttattggcg cgcacgccga tggtttcgct
     6121 ggtttgctca aacgcatcac tcacatggcg gaatgcatcc accagctcgc cttcactcat
     6181 ctcaccatcc agacgctcgc cgttcagcgt ggcaaccgcc agcagaaaag agagtcggtc
     6241 taccggcagc gagatggaga agtcattttt tctggcccag gcaaccagtt cggggactgt
     6301 ctgggaaaat tcactcatac tttatccttg ctctgcggtt tgcgcgcggt gacatgaata
     6361 taacgcccta gggtaatata cggttcctga cggcaataac gcgtttctaa ttcaagtaat
     6421 gcttcatagc agtcgcgctg ctggtacttc tcgcgcagat aatcatgaaa cacgcgaacg
     6481 cctgtcttac ccataatttg ccaaccagct tcttccagcc acagataaac ctgcgccggg
     6541 tcgcgtggat aatctggcga aagcgtccgt tttttctttt tcggcattcc cgcctgcacg
     6601 taatcaaaat tcccggcgac catgttatgc atcaacaaac catgcgcatt gtagaacatt
     6661 aacgacaaca ctccgcctgg acgcaacact gaccacaggg tctgcaatac actgcgggga
     6721 tcggccaccc actcgagcac cgcatggaac aatatcagat caacgggcgt ttccaaatgc
     6781 gaagcaacat cctgagcggc gcaatgtata aattgcatgt tgtcgctcac accttttgct
     6841 tctgccgcct gttttgcgcg gtcgatcatc tgcgcagaaa gatcgcataa aataacctga
     6901 tgcccgcgct cggccatttt gattgcggtc tgcccttctc cgccgccagc atccagcaca
     6961 cgcagttttt gcgggcccat ttccgccagc acgcgatcga gatcctgcca cagaatagcc
     7021 tgtcgaagct gccctttggt ggtgccgtaa atgttacggg aaaacttttc cgcaatatca
     7081 tcaaaattgc gatcctgcat tgctttctcc accggttggc taagacgttt tctgataaag
     7141 acgctatttt gtcacagcca cggcgagaat gaacccgtct ggtgtaatat cgctagcgat
     7201 cccttgctat atggttaaaa aaggaaccag aaaggatgct ttttacactg aaaaaagtga
     7261 ttggcaatat gttgctgcca cttccgctga tgttgttaat tattggcgct ggcctggcgc
     7321 tgctgtggtt tagccgtttt caaaaaaccg ggaagatttt tattagcatt gggtggctgg
     7381 cactcttgtt attgagtttg caaccggtag ccgaccgcct gctgcgtccc atcgaaagca
     7441 cctatccgac atggaataat tcgcaaaaag tggactatat cgtggtgctc ggaggtggtt
     7501 atacctggaa cccgcagtgg gcaccgagct ctaatttaat caataacagt ctgccacgcc
     7561 tgaatgaggg tatccgttta tggcgcgaaa atccgggttc aaaactgatc ttcacgggag
     7621 gcgtagcaaa aaccaatacg gtgagtacag cggaagtagg tgccagagtt gcgcaatcgc
     7681 tgggcgtacc gcgcgagcaa attatcaccc tggatttgcc aaaagatacc gaagaagaag
     7741 ctgcggcagt gaaacaggcg attggtgacg cccctttcct gctggtgacc tccgcatcac
     7801 atctgccgcg ggcgatgatc ttttttcagc aggaagggtt aaatccgcta ccagccccgg
     7861 cgaatcagtt ggccatcgac tcgccgctta atccgtggga acgggcaatc ccttctccgg
     7921 tctggctgat gcatagcgat cgtgttggct atgaaacgct tggccgcatc tggcagtggc
     7981 tgaaaggtcc gtcaggcgag ccacggcaag agtgattttg atgccagatc gaagttgcgc
     8041 cgactaaatc gtccggtatt aaccagttgc gcaacttcat cccataacac atacaaccaa
     8101 cgccgccaga tgaacgactc cgccactggc gcgcgttgca ggtaactcca gagcaaatct
     8161 tccgccagag aattatccat tagtcggaac agttcgtatt ctcgcggtgc ccaaagcatt
     8221 agtcccggcc cgaccatcgc cagtaactga tcgctgcgcg aatctttcag catgctgcgt
     8281 aaacagaagt taccgtgaat caacacacaa ttgtcgttaa agccttcaaa taatgccggg
     8341 agacattcgc gagtgcgaaa caggatccgc ttatcctgca tcgtcagacc ggtgttattg
     8401 aactgattga gcgtggtcca tagcacttca acatgttgcc ggtaccatga gggccagaaa
     8461 ttttcctgag tattgtcgac cgcgccgacg caaccgcgac tgtcctgacg gtgccaggcc
     8521 agtaaggctt caacgatttg gtctttgagc tgttcccagc gttctggtgt tcgggctggt
     8581 gcctccaccg aaacaccacg catccgctcc agtagcagga catcagggcc ggggtgttct
     8641 tcatgcgtca tcacaccata aatagtcggc atacggacag tcccacttct tgccagcatg
     8701 gtggttttcc atgccagttg tcgggctttt ccgggcgtac taaagcttct tgccattaac
     8761 ggcattgggt ttccctggct gtcatacaaa gcccacaacg ccgtatccgc tttttcattg
     8821 acgcactcaa tacgactgag tttttcgccc agtaaatggc ttaattcggc acgcagctgt
     8881 tccattcaga ttaccctcat aaaagctact gctttcataa tgagcgccaa tataaaagat
     8941 gtcacctgaa tgagatcaat tgttgaaaag aaaaattgag gaaaatcgta acggtacgac
     9001 actcctccca aaattggctg aagtgtcgtg aagtgaaatt agcgcatttc agcgcgaacg
     9061 cgctcaagat cttcaggggt atccacacct gtgccaggaa cttcctgagc aacagcaaca
     9121 tggatttttt cgccgtacca cagaacacga agctgctcta acatttcgat gtgttctaac
     9181 ggacttgcct gccagttgac gtaacgacgg ataaagcctg cacggtagcc ataaatacca
     9241 agatgacgca ggaagttatc gccaacggtt tcaaggcctt ctgcaaaacg atcacgatcc
     9301 caaggaatgg tggcacgtga gaagtacagt gcataccctt cagcgtcgag aaccactttc
     9361 accgcattcg ggttaaacgc ttcttccgca ttgtggattg gcaccgccag agtcgccata
     9421 cccacctgac gctgagcgag gttatcagca acctgacgaa tgattgtcgc agggatcatc
     9481 ggttcatcac cctgcacatt aacgatcacc gtgtcgtcgc tgaatgcgca tttttcgaca
     9541 acttccgcca gacgttctgt tcctgactga tgatcggcgc gcgtcataca tacttcaccg
     9601 ccagcggctt caacggcgcg ggcaacatca tcatgatcgg ttgccacgat gatgcgctcg
     9661 gcacctgatt cacgcgcgcg ttcaagaaca tgaacaatca tgggtttgcc gttaatatca
     9721 accaatggtt taccgggcag acgcgtggac gcgtagcgcg cgggaataat gaccacaaaa
     9781 ctcatgattt actctcatca gcagtcagca cgcgggcttc cgtttccagc aacactggaa
     9841 tgccatcgcg gagcgggaaa gccaggttat ccagtttgca aataagctct tgtttttcct
     9901 ggttatacca aagttttccg ttgcaaaccg ggcaggcaat gatttcaagc aaacgatgat
     9961 ccatagttcc tccagatgga tcgggttatg aatgcataaa tcttatcata atcatttatg
    10021 cgacggggtc tatttcccag ccagtgcggc aatcagtaaa gagaccttgc gggcagcgtc
    10081 ctaatgtcac ccgcgttgcc tgctgccagc tcgcgaaatc agtaatcgcc tgacgtaacc
    10141 ctttttgcag cattgtcgtt ggtttaatgc cttcctgtaa ccacagagag ataacttcaa
    10201 ggatgcctgt ctggcgatgc attttggcat ccattcgccc aactaattgc ccacgatgta
    10261 ataatggcag aacaaaatag ccatactggc gtttcggcgc tggggtatag cactccagcc
    10321 ggtagctaaa atcaaaaagc tgctctgcgc gtttgcgatc ccagacaaca ggatcaaaag
    10381 gcgaaagtac cgcgctgtgc gttgcagtga gctttccggc cagcgcacgc tcgagtagcg
    10441 gcagcaaatc agcatgcagc caaagattgc ccaatttttc aacatgcaca gcaatgattt
    10501 gctgctgttc agccctcgct tcgcgccacg ccgccagtgc cggacgtttc agccgatagt
    10561 aatctgccag ccactgttcg cggaatattc ccaggctacg cgcactgtta tccagcatga
    10621 tgatttctgc ttctgtttgc gaaacgagat cgcgctcatc atcccagtca ggcatgacac
    10681 ggtgggttaa atcataaacg cgctggaagt tgcgccgttc aatcaccatc acctttccgg
    10741 cagtaaataa accttccaga tgccgtttat gcggcttcca ttcccaccag ccgcttgcac
    10801 ctttacgagg atgctcaaaa tcggctgaac gtaccggccc tttatcatga atatgctgaa
    10861 ttaactgtgc aatttccgcc tcatgttcct gcatccaggc gtctttgtat ttccagccca
    10921 ttttttcagg tgccagcatg cggtggcgaa taagacgaaa gtcgctacgc ggcataaagc
    10981 aggcttcatg cgcccagtat tccattaatt cgccacgcgc cagagactca tccagccact
    11041 gggcaggata atttcccaga cgactgaaaa gcaccagata tggactacgg gcaacaatat
    11101 tgatggtatc gatttgcagc aaggacatgc gggagatcgt tgccggaata tcctccaacg
    11161 acgctcgacg gcggggtttg tttaacaggc cttgtgcggc aaggtgaaga ttacgcgcat
    11221 cagcaaggga gaggtgcggc agcgacattc atgactccat caatcgaacg ctgccgcggc
    11281 gtaactagtt gccagaagcc agcgaggtta gttgcgtaag cagtttcgct ggttcatcac
    11341 ctgaaagctg tgcgtctaca ggcaaatacc accaattttc ttctgcaaag gcccggcatt
    11401 tcaccgcatc tttttcagtc attaccagcg tttgcccggc gcttaccaac gcactgacat
    11461 cctcatggtt caaagactga tgatcggcca gcggtacaca tttttccggt tgtacgccac
    11521 acatcttcag cgtggcaaaa aagcgcggcg gatgcccaat ccccgccatc gccactacat
    11581 gttcaagctg agcaacgtca caacgcgtac cggtacgtaa attcaccgcc tgacccggca
    11641 gcagatgcat ggggatttca ccgctgcgag ggacaccgcc gttgacgatt accgcatcaa
    11701 ccgactttaa gcgccccgct cgctcacgca ttggccccgc cggcaaccac cagccattgc
    11761 caaagcgacg cacaccatca ataacgacaa tttccacatc acgcgccaga cggtaatgct
    11821 gtaaaccgtc gtcggttacg atgatctgca catcagggtg ttgcgccaga atggctttta
    11881 ccgcatcaga acgaacggga gaaaccgcaa caggcgcatc agtgcgttga taaatcaaca
    11941 caggttcatc acccgcctgt gctgtggtgg tatctgccga caataacagc ggataagatt
    12001 cagccttacc accatatccc cgcgatacga cccccacgcg aataccgcgc tgttgcaact
    12061 gttccaccag ccagacaacg accggggttt ttccgttgcc gcctgcggtg agattaccaa
    12121 ccacgacaac cggtacgggg gcacgccagg cgcgcttcag ttttagttta tagcaaagac
    12181 ggatcgcgcc actcaccagg ccatacaacc aggagagtgg cagcaatagc cgccacaaag
    12241 gggattcacc agaccagatt ttttcgatca ttggccaaac tgcattttgt gaagttgcgc
    12301 gtaaacgccg cggtgctcaa gcaaatcgtt atgcgtaccg cgttccacaa tgacaccatc
    12361 ctcgacgacc acgatttcgt ctgccttttc aatggtagac aagcggtggg caatcaccag
    12421 agaggtacgg tttttctgca actcatccag tgccgcctga atcgcacgtt cggattcggt
    12481 atccagagcc gaggtagctt catccagaat cagaatcggg ctatcacgca gcaaggctcg
    12541 agcgatagca atacgctgac gctgaccgcc agagagcagc acgccgtttt caccaatcac
    12601 tgtatcgaga ccgttatcca tcttattgat gaagtccatg gcgtaggcca tccgcgccgc
    12661 ttcttcaatt tgctcacggc tgtactgttc agtccgtgcg taagcaatgt tgttagcaac
    12721 cgtatcgtta aacagatgga cattctgcga caccagagca acctggttac gtaacgacgc
    12781 cagggtatac tcgcgcagat cgtgaccatc catcaggatt tcgccttcat caatatcgta
    12841 aaaacgcgtg atcaggctgg cgatggttga tttacctgaa ccagagcgtc caaccagagc
    12901 aaccgtcttc cctgccggaa ttttcaggtt gatgttacgc aatgcgggta cgtcacgtcc
    12961 cggataagta aaggtgacat tgcggaattc cacgtcgcca gtcgcacgct cgatcacgcg
    13021 cttaccttcg tctttctcct gctcactgtc cagaatggta aacagcgtct gacaagccgc
    13081 cataccgcgc tggaactggg cgttaacgtt ggtcagcgat ttcagcggac gcatcagtgc
    13141 aatcattgaa gagaaaacaa cggtaatcgt accggcagtc aggttatcca taacacttgg
    13201 gaagctcgcc gcatacagaa caaacgccag cgccaaagag gcgatcagct gaatgatcgg
    13261 atcagagatg gaagaggctg aaaccatttt catcccctga agacgcattc ggttactgac
    13321 tttatcgaag cgtttcgttt ccacttcctg accaccgaaa atcaatactt ctttgtggcc
    13381 cttcagcatt tgttctgcac tggtggtcac ttgccccatg gtgttctgca tgtttttact
    13441 gatgttgcga aaacgcttcg atactacgcg aatcgcaatc gaaacaatcg gtgccagcac
    13501 aatcaaaatg atcgacagtt gccaactgta atagaacatc atgatgaaca ggccgatgat
    13561 cgacgcacct tcacgcacaa cagtaatcag tgcgccggaa gaagaagaag caacctgttc
    13621 ggaatcgtag gtgatacgtg acaacagcgt acccgttgac tgtttgtcaa agaatgaaac
    13681 tggcattccc atcatgtgac caaacaggcg gcgacgcatg gtcattacca cctttcctga
    13741 tacccaggag atacagtagc tggagacata gctggtgata ccacgtaaaa tcatcagtcc
    13801 gatcaccacc agcggcatcc acaccagcac ggagcgatct gttttaccaa agccatcatc
    13861 aagaagtggc ttaaggagcg ataacatgaa ggtatcgctg gctgcgttga ggattaacgc
    13921 tacgcccgcc acgatcagac ccgctttgaa aggcgcaatg gttggccaca gtcggcggaa
    13981 tgtctgccac gtagagagat ctttgtcgtt atgcattcaa aaaaccagca tttgttgaaa
    14041 tagccgcata ttctacccgt tatccactgg cacgccaaac cactgatgat accaacgagg
    14101 taaaatttgc tctctgaggc tgctaatccg ccagccttgt gctgaaaaat tgaccgtcac
    14161 ttgcccctga tgtggagtat caagccattg atatcctttc tgttgatagc gatgcttaac
    14221 tttgttagag ggcaatcgcc atgcgttata gcgcgatgcc gatgcgagtg ccacttttcc
    14281 attcactcgc tgaattaatg gcaacgatga tgaggtatta ctgccatggt gaggtacctg
    14341 aagcaatgtt gcctgcactt gctgccagta acggcttagc atcttttgtt cagctggggc
    14401 ttcaatatca ccggttagaa gaatgctatt cgtcccgtca tcaaccttaa ccacacagga
    14461 atggttattt cccttatcgt tgctaccttg taaaggccag tgcgcgctga aacgcaatcc
    14521 ttgccactgc cacgcttcgc cacgcacaca gggctgatga tgttcccagt ttaacggact
    14581 tctgatccat aacatcggcc atatatgcaa tattgaatcc agccctcccc ggtgatccag
    14641 atgttcatgg ctcagaataa cgccttccgg ttcaagatta tgccagtgga gccaggggat
    14701 aatcagttgt tgcccactat ccccttcagg ccagtcctgt gtcatagaga atcgctttgc
    14761 cgtttctggc tatcaccatt gccagccctt gcccgacatc aagcatgtac agctgccact
    14821 cgtcaggtcg aggtttttgc cacagcggcc agcacatcag caagcctcca gccacacaca
    14881 ttgctggcaa cgttcgccag gcgtttaatc gccataccac cagtaagaac catggggaaa
    14941 atgatagcca ttgccaacat tcaacaatgt tgatccaccc ttccggcaaa ctctttaacc
    15001 cccagaaaag taaagccaaa gaccggtcgg caagaaacca taacccctgt tccaggatta
    15061 acggcccgct aaaatgcaca accatcgcgg cgaggatcaa cggaaccgtg ataaatgtca
    15121 ccaagggaat tgcaaataga tttgcaataa acgaggtcag actaatgcca tgaaatatga
    15181 cgatttgcat gggcataagc aggagtgtga ttcccagttg cagatggatg agggaaacaa
    15241 ctgcacgcaa taccggcggc agttgccact caggacaggg aaaccactga taccaaaaaa
    15301 tcagtgccgc gaccgcagcg gcagagagcc ataaactttg cgagagaatg gcaacaggat
    15361 ccatcagcaa aattgccgcc agacaacata tccatacatc ccagccactc cactgtcgcc
    15421 cacttaactt aagcattccc cacgtagcaa gcgccaccac ggtacgcaat gcaggaggtt
    15481 gcattcccgt caaccaggca taaaaagcag cacagcagat tccgccaatt aatggtattt
    15541 gccagtggat ccagcgccca ggcagaaaaa tttgtccact gcgaatgagt ccggcagcca
    15601 gcaacgccgc aaaagcgatg tgcaatcccg atatcgccat taaatgcgcc gttccagtat
    15661 cgcgcatgat atttttgatt tctttgggga cggataaccg ttcccccata cctaaaccaa
    15721 gaataaccgc attccacgga tagggttgca gcgttgtttg tagtgacgcc agatactgtg
    15781 cacgcaggct acaattcggt tcaatgacac ttgcctgcag aaaacggccg gtgagcggct
    15841 gatgctgggc aatggcataa cgctggctat caaagccgcc atcattaagt tggccatgaa
    15901 ctgcacgaac tttgagtttc attgaccatt gttgtccagc acaaaccgct tgcggaagtt
    15961 attcgccata cagcacaagg cctggcgcag ggaatatacg tcgaccttgt agataagtaa
    16021 tttgaccgta atgagtggtc atgccgtcag ttgcagtgat ctcaacaatt gcatcctgcg
    16081 tcgcgccagt taaggtttct cctgcccaca aaatttgctt tgctgataat atgccccaaa
    16141 cgaaaaacag caacgtcagc gcgatataac ggacggtttt aacagggata aatgccagta
    16201 cgcaggcgaa gagagtcaga aacgcaaggg ttaatgtcct aggcaattgg ggcaaaatca
    16261 gcaacggaaa aattccgcaa attatgcata cgccgactgt cgttattttc atgcacccct
    16321 ccctgtttat ggaaagtgtg caactttgtt gagcggttgt ctggtgggaa ttttgcgttc
    16381 cccgaggcac attcagaaga atttaacgaa agggtgaaaa ctgctcaaaa ctctgcgagg
    16441 cagcttccag ttcctgatta gagaattgaa aaaagcaccc gacaggtgct tttctcgcgt
    16501 tcaagtttga gtaaaaactt aaccgtaaat attggcgcga tcgcgcagtt ctttaccagg
    16561 tttaaagtga ggaacgtatt ttccttccag ttctacttta tcgccagtct tcggattacg
    16621 tccggtacgt ggtgcgcggt agtgcaaaga gaaactgccg aaaccgcgga tttcaatacg
    16681 ctcgccctgc gcaagagtcg aggccatatg ctccagcatc tcttttactg catcttcaac
    16741 cgtcttggcg ggaatgtgcg attgctgggt ggcaagtctt tctatcaatt ctgacttggt
    16801 catgattcct ccggttcctt aaaggcaaat taagcggctg ctgttgctgc attgattaag
    16861 ggcggccgta gccgccctta gtgcttgatt acaggacgaa acctgcaatc tgtcaagtaa
    16921 actcaacaaa cttcggaata aaaatcccga agagtcagag aattactcgc ctttagctgc
    16981 tttgaaagct tcagccattg cgttgttgga gaagtttgca tcttcctgtt tgttaacagt
    17041 tgcgattgca tctttctcgt cagcttcgtc tttcgcacga acagacaggc tgattgcgcg
    17101 gtttttacga tcaacgccgg tgaatttagc ttcaacttcg tcgccaacgc tcagaaccag
    17161 ggtagcgtct tcaacgcggt cacgggatgc ttcagaagca cgcaggtaac cttcaacgcc
    17221 gtcagccagt tctacggttg cgcctttagc gtcaactgca gttactttac cggttacgat
    17281 agcgcctttc ttgttcagag caacccagtt gttgaacgga tcttctgcga gctgtttaac
    17341 gcccagggag atacgttcac gttctgcgtc aacctgcaga acaactgcag cgatttcgtc
    17401 gccttttttg tattcacgaa ctgcttcttc gcctgcaacg ttccaggaga tgtcagacag
    17461 gtgaaccagg ccgtcgatgc cgccgtccag gccgatgaag ataccgaagt cagtgataga
    17521 cttgatttta ccttcaacac ggtcgccctt gttgtgggtt tccgcgaact gctgccacgg
    17581 gttagctttg cactgtttca gacccaggga gatacgacga cgttcttcgt cgatatccag
    17641 aaccataact tccactacat cgccaacgtt aacaactttg gacgggtgga tgtttttgtt
    17701 ggtccaatcc atttcggaaa cgtgtaccag accttcaacg ccttcttcga tttcaacgaa
    17761 gcagccgtag tcagtcaggt tggtcacgcg accagtcagt ttggtacctt ccggataacg
    17821 tttagcgata gctacccacg gatcttcgcc cagctgtttc aggcccaggg atacacgggt
    17881 acgttcgcgg tcgaacttca gcactttaac agtgatttcg tcgcccacgt tgacgatttc
    17941 gctcggatgc ttaacgcgtt tccaggccat gtcagtgatg tgcagcaggc cgtcaacgcc
    18001 gcccagatca acgaatgcac cgtagtcagt gaggttctta acgatacctt taacttccat
    18061 gccttcctgc aggttttcca gcagctgatc gcgctctgcg ctgttttcgg attcgataac
    18121 ggcacgacga gaaacaacaa cgttgttgcg cttctgatcc agcttgatta ctttaaattc
    18181 aagctctttg ccttccaggt gcagagtgtc acgcaccgga cgaacgtcta ccagagaacc
    18241 tggcaggaac gcacgaatac cgttcagctc aacagtgaag ccgcccttaa ctttgccgtt
    18301 gataacaccg gtaacagttt cagcatcttc gtaagctttt tccagcgtga tccaggcttc
    18361 gtgacgttta gctttctcac gggacagcag agtttcaccg aagccgtctt ctactgcgtc
    18421 cagagcaacg tcaacttcgt cacctacctg gatttccagc tcgccctggg cgtttttgaa
    18481 ctgctcagcc gggatggcgg actcagattt cagaccagcg tcaaccagta ctacgtcttt
    18541 gtcgatagca acaacaacgc cacgaacgat agaacccggg cgggtttcga tttcttttaa
    18601 ggactcttca aagagttgag caaaagattc agtcatgttt aatcttcagg tttatattta
    18661 acgtccacct ggctccatgc cggatggggt tgtttaacat acccgctgtc attccattgc
    18721 aacgggggta ctgcaaattc ggtcgcttat gcgagagcca atttctggcg cgcgtattgt
    18781 agcgcttttt caatcaattg ctcaatgctt aaggtggtgg aatccaacac taaagcatcg
    18841 gctgccggaa ccagtggcgc taccgctcgg ttacgatcgc ggtcgtcgcg ttctttgatc
    18901 tcggccaaaa ggcgctcgaa gttaacacta aagcccttct cctgcaactg tagcatgcgg
    18961 cgatgcgcac gttcttccga ggaggcgtca aggaaaattt tcactggtgc atcagggaat
    19021 accaccgttc ccatgtcgcg gccatcggca atcagacctg gtaattcgcg aaacgcgcgt
    19081 tggcgacgca ataatgcttc acgaacgcgt gggaatgccg cgacttgtga agccgcattc
    19141 gccacttcct gagtacgaat ttcgccgctg acatcttccc cttcgaggat cacttccaga
    19201 ttgccattgg tcgacacaaa acgtacatcc agatgggatg ccagcggtac cagcgcatct
    19261 tccgacgcaa catcaacatg gtgatgtaat gccgccaatg ccagtacgcg ataaattgca
    19321 cccgagtcca gcagatgcca ttgcaacgct tccgccatag ccttacacaa ggtgcctttc
    19381 cctgcaccgc ttgggccatc aatggtaata accggggcaa ttgccgtcat ctttatctcc
    19441 ttaaaacagg cgtaccgtta acataaccgc gcgcattata cgcgccgacg ccacaaatcg
    19501 ttaacgttgt gtgcaaaaac cagttttgtc tgtattcaga tggggattta ttgcgcaatt
    19561 atagcgggct ggcagaagac cagcccgaaa gatgacaatg atttacttac cagaggcgat
    19621 acgatcacgg atgtgttgcg cacgctctgt tgatggtggg tgagagtcaa acatggactg
    19681 ggtgcgaccg ccatccaggc tagccagttt ttcaaagctg ccaaccagcc cctgggtgct
    19741 gatgccacgt ttcttcaaca gatcaaaaga gaaatcatct gcttcggact ctttatcacg
    19801 ggagtattta gcattaatag cgccttctgc gatatcaccc aattgtgagc gggaaagctg
    19861 ggaagccaca ccgctggtag ctgaaatggc atcgcgtgcg gcaacgatcg cataagaagc
    19921 tttcatttca gccagcgagt gacccaacgc gacgtggccc agttcatggc ccagaacacc
    19981 ttcaatttcg ttgtcattca tcatgtccat caggccactg tagacacgaa cacagccgtt
    20041 cgccatcgcc catgcgttga cgtcgctggt catataaacc ttatagttga ccggcgtgcc
    20101 attaatattg ttacccaatg ctttggcgat ttttgccaga cgtttaccgt acttgctgga
    20161 tttacttgcg acttgattgc cgctgtccat ttcggcacag ccctgattgg caatcgcttt
    20221 tgcatcggca tcgcttaatg ttgctgcttt ataggcattt aaaccggagc tgatagccat
    20281 attggtatca ataccgtggg tattttgaca ccctgtcagt aatgctgcag aggtcgcaat
    20341 cgccagcagt aatttagtat tcttcatgtg aatcatccgt tgattaatca tatataaata
    20401 ttatgtatca acaacttaca ttgacaagcg acgctaccat taatcaaaat gactcacaag
    20461 gtccgaaaaa aaacgcc
//
LOCUS       DAJTQQ010000069        20442 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000069 DAJTQQ010000000
VERSION     DAJTQQ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.069"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            143..484
                     /gene="raiA"
                     /locus_tag="OO309_002731"
     CDS             143..484
                     /gene="raiA"
                     /locus_tag="OO309_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR5847823.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    587..712
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            588..635
                     /gene="pheL"
                     /locus_tag="OO309_002732"
     CDS             588..635
                     /gene="pheL"
                     /locus_tag="OO309_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCR5847824.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            734..1894
                     /gene="pheA"
                     /locus_tag="OO309_002733"
     CDS             734..1894
                     /gene="pheA"
                     /locus_tag="OO309_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR5847825.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(1937..3058)
                     /gene="tyrA"
                     /locus_tag="OO309_002734"
     CDS             complement(1937..3058)
                     /gene="tyrA"
                     /locus_tag="OO309_002734"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR5847826.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3069..4139)
                     /gene="aroF"
                     /locus_tag="OO309_002735"
     CDS             complement(3069..4139)
                     /gene="aroF"
                     /locus_tag="OO309_002735"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR5847827.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4349..4714
                     /locus_tag="OO309_002736"
     CDS             4349..4714
                     /locus_tag="OO309_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR5847828.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4864..5382
                     /locus_tag="OO309_002737"
     CDS             4864..5382
                     /locus_tag="OO309_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR5847829.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5372..6597
                     /gene="dgcN"
                     /locus_tag="OO309_002738"
                     /pseudo
     CDS             5372..6597
                     /gene="dgcN"
                     /locus_tag="OO309_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6613..7095
                     /locus_tag="OO309_002739"
     CDS             6613..7095
                     /locus_tag="OO309_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR5847830.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7172..7519)
                     /gene="rplS"
                     /locus_tag="OO309_002740"
     CDS             complement(7172..7519)
                     /gene="rplS"
                     /locus_tag="OO309_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR5847831.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7561..8328)
                     /gene="trmD"
                     /locus_tag="OO309_002741"
     CDS             complement(7561..8328)
                     /gene="trmD"
                     /locus_tag="OO309_002741"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR5847832.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8359..8907)
                     /gene="rimM"
                     /locus_tag="OO309_002742"
     CDS             complement(8359..8907)
                     /gene="rimM"
                     /locus_tag="OO309_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR5847833.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(8926..9174)
                     /gene="rpsP"
                     /locus_tag="OO309_002743"
     CDS             complement(8926..9174)
                     /gene="rpsP"
                     /locus_tag="OO309_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR5847834.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9311..10672)
                     /gene="ffh"
                     /locus_tag="OO309_002744"
     CDS             complement(9311..10672)
                     /gene="ffh"
                     /locus_tag="OO309_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR5847835.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10839..11630
                     /locus_tag="OO309_002745"
     CDS             10839..11630
                     /locus_tag="OO309_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR5847836.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11651..12937
                     /locus_tag="OO309_002746"
     CDS             11651..12937
                     /locus_tag="OO309_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5847837.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(12992..13585)
                     /gene="grpE"
                     /locus_tag="OO309_002747"
     CDS             complement(12992..13585)
                     /gene="grpE"
                     /locus_tag="OO309_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR5847838.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13708..14586
                     /gene="nadK"
                     /locus_tag="OO309_002748"
     CDS             13708..14586
                     /gene="nadK"
                     /locus_tag="OO309_002748"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR5847839.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14672..16333
                     /gene="recN"
                     /locus_tag="OO309_002749"
     CDS             14672..16333
                     /gene="recN"
                     /locus_tag="OO309_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR5847840.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16482..16823
                     /gene="bamE"
                     /locus_tag="OO309_002750"
     CDS             16482..16823
                     /gene="bamE"
                     /locus_tag="OO309_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR5847841.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16885..17175)
                     /locus_tag="OO309_002751"
     CDS             complement(16885..17175)
                     /locus_tag="OO309_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR5847842.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17165..17602)
                     /gene="ratA"
                     /locus_tag="OO309_002752"
     CDS             complement(17165..17602)
                     /gene="ratA"
                     /locus_tag="OO309_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR5847843.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17773..18255
                     /gene="smpB"
                     /locus_tag="OO309_002753"
     CDS             17773..18255
                     /gene="smpB"
                     /locus_tag="OO309_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR5847844.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18470..18832
                     /gene="ssrA"
                     /locus_tag="OO309_002754"
     tmRNA           18470..18832
                     /gene="ssrA"
                     /locus_tag="OO309_002754"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19037..20278
                     /locus_tag="OO309_002755"
     CDS             19037..20278
                     /locus_tag="OO309_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5847845.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5282 a         5344 c         4800 g         5016 t
ORIGIN      
        1 aaaaaaacac ctcttcctgc gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa
       61 attatgagat ttccatcaca cattttgaca tcaggaacgg tatgctgaat tcaccaagac
      121 gggaagacaa gaggtaaaat ttatgacaat gaacattacc agcaaacaaa tggaaattac
      181 tccggccatc cgccaacatg tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca
      241 tctgattaat ccacatatca ttctgtccaa agagccacaa gggtttgttg ctgacgccac
      301 aatcaataca cctaacggcg ttctggttgc cagtggtaaa catgaagata tgtacaccgc
      361 aattaacgaa ttgatcaaca agctggaacg gcagctcaat aaactgcagc acaaaggcga
      421 agcacgtcgt gccgcaacat cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga
      481 gtagtccttt atattgagtg tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc
      541 gtgaaaacag tacgggtact gtactaaagt cacttaagga aacaaacatg aaacacatac
      601 cgtttttctt cgcattcttt tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa
      661 cagaatgcga agacgaacaa taaggcctcc caaatcgggg ggcctttttt attgataaca
      721 aaaaggcaac actatgacat cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc
      781 gctggatgaa aaattattag cgttactggc agaacggcgc gaactggccg tcgaggtggg
      841 aaaagccaaa ctgctctcgc atcgcccggt acgtgatatt gatcgtgaac gcgatttgct
      901 ggaaagatta attacgctcg gtaaagcgca ccatctggac gcccattaca ttactcgcct
      961 gttccagctc atcattgaag attccgtatt aactcagcag gctttgctcc aacaacatct
     1021 caataaaatt aatccgcact cagcacgcat cgcttttctc ggccccaaag gttcttattc
     1081 ccatcttgcg gcgcgccagt atgctgcccg tcactttgag caattcattg aaagtggctg
     1141 cgccaaattt gccgatattt ttaatcaggt ggaaaccggc caggccgact atgccgtcgt
     1201 accgattgaa aataccagct ccggtgccat aaacgacgtt tacgatctgc tgcaacatac
     1261 cagcttgtcg attgttggcg agatgacgtt aactatcgac cattgtttgt tggtctccgg
     1321 cactactgat ttatccacca tcaatacggt ctacagccat ccgcagccat tccagcaatg
     1381 cagcaaattc cttaatcgtt atccgcactg gaagattgaa tataccgaaa gtacgtctgc
     1441 ggcaatggaa aaggttgcac aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc
     1501 tggcggcact ttgtacggtt tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa
     1561 cttcacccga tttgtggtgt tggcgcgtaa agccattaac gtgtctgatc aggttccggc
     1621 gaaaaccacg ttgttaatgg cgaccgggca acaagccggt gcgttggttg aagcgttgct
     1681 ggtactgcgc aaccacaatc tgattatgac ccgtctggaa tcacgcccga ttcacggtaa
     1741 tccatgggaa gagatgttct atctggatat tcaggctaat cttgaatcag cggaaatgca
     1801 aaaagcattg aaagagttag gggaaatcac ccgttcaatg aaggtattgg gctgttaccc
     1861 aagtgagaac gtagtgcctg ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc
     1921 atccggcact ggattattac tggcgattgt cattcgcctg acgcaataac acgcggcttt
     1981 cactctgaaa acgctgtgcg taatcgccga accagtgctc caccttgcgg aaactgtcaa
     2041 taaacgcctg cttatcgccc tgctccagca actcaatcgc ctcgccgaaa cgcttatagt
     2101 aacgtttgat taacgccaga ttacgctctg acgacataat gatgtcggca taaagctgcg
     2161 gatcctgagc aaacagtcgc ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga
     2221 gcgccagaag ttgctcaagc tgaacatttt cttctgccag gtgcagcccg taagcaaaag
     2281 tagcaaagtg gcgcagagcc tgaataaacg ccatattctg atcgtgctcg acagcgctaa
     2341 tacgatgcaa ccgagcgccc cagacctgaa tttgctccag aaaccattgg tatgcttccg
     2401 gcttacgtcc atcacaccag accacaactt gctttgccag gctaccgctg tccgggccga
     2461 acatcgggtg taaccccagt accgggccat cgtgcgccgc cagcatggcc tgtaatggtc
     2521 catttttcac tgatgccaga tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc
     2581 cgataacttg ctcagtaacg tggattggca cactaacaat caccattccg gcatcggcaa
     2641 caatatcagc cgctcgatcc cagtcatgtt gctccagaat ccgcacctga taacccgata
     2701 gtgtcagcat cttctcgaac aggcgtccca tctgaccgcc accgccgacg ataaccaccg
     2761 gacgcagtga cggacaaagt gttttaaatc ctttgtcgtt ttcactggag taagattcac
     2821 gcatcacccg acgcaaaaca tcctcaatca gatctggcgg tacacccaga gcttccgcct
     2881 ctgcacgacg cgaggccaac atagatgcct cgcgctccgg aacataaata ggcagtccaa
     2941 agcggctttt caccttgccc acttcagcaa ccagttccag acgcttcgct aataaattca
     3001 gcagcgcttt atcgacttca tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa
     3061 taaacctctt aagccacgcg agccgtcagc tgcccgttca gatcctgatg aatttcacgc
     3121 agcaaggcat cggtcatttc ccagctaatg caggcatcgg ttacggatac accgtatttc
     3181 atttcactgc gcggttgctc agaagactga ttgccctcgt ggatattact ttcgatcatc
     3241 agaccaataa ttgagcgatt accatctttg atttgagcaa ccacggattc tgccaccgca
     3301 ggctgacggc gataatcttt attggaatta ccgtggctgc aatctaccat cagagacggg
     3361 cgcagtcccg cctgttccat ctctttttca cattgcgcaa catccgcagg gctatagttc
     3421 ggcgctttac caccgcgcag gatcacatgc ccgtccggat tcccctgagt ttgtagcaac
     3481 gcaacctgcc ctgcctggtt aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata
     3541 gcgttaattg ctgttgccag actgccgtcg gtgccgtttt taaaaccaac cggcatggaa
     3601 agcccggagg ccatttcacg gtgagtttgc gattccgttg tacgagcacc aattgctgac
     3661 cagctaaaca gatcgcccag gtattgcggg ctattcggat ctaacgcttc cgtcgccagt
     3721 ggcagtccca tattcaccag ctcaagcaac aatttacgcg cgatttgcag cccggcttct
     3781 acatcaaaag agccatccat atggggatcg ttaattaacc ctttccagcc gacagtggta
     3841 cggggttttt caaaatagac gcgcattacc agatagaggc tatcgctgac ctctgcggca
     3901 agagctttaa atcgacgagc atattccaga gcagtttccg gatcatgaat ggaacaagga
     3961 ccacatacta ccagcagacg aggatcgcgc ccggcgataa tatctgaaat ggttttacgc
     4021 gagtcagcaa tctgggcttc ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc
     4081 ggagtcatta aaacctgttc gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg
     4141 atggcgatcc tgtttatgct cgtttgcgat agttgctcct cagcgaggat gacgtaacga
     4201 taacacataa agtaaagttt tcaatccata tttcgtacat ttttatttac acaggcaatt
     4261 tagtcgcgct ttcaaccctt gcttctgtat agataaattt acatcccctt taaaaacaat
     4321 ccgctatgct ttgaaaaagg agaaagaaat gatgaaaaag tttatcgccc ccttgttggc
     4381 tttactggtt agcggatgtc agattgatcc ttatactcac gcgccaacct tgaccagcac
     4441 cgactggtat gatgtcggta tggaagatgc gatatcgggc agcgccataa aagatgacga
     4501 tgcatttagc gattcacagg cggatcgcgg tctatacctt aaaggatatg ccgaagggca
     4561 aaagaaaact tgccagaccg attttactta tgcccgggga ctttccggta aaagctttcc
     4621 tgccagctgc aacaatgttg aaaatgccag ccaactgcat gaagtatggc aaaaaagggc
     4681 tgatgaaaat gccagcacaa tacgattaaa ttaattaaaa ccccacaaat aaattaaaca
     4741 taagatttta ctcattgtgt tttatttctc acattgatga cggtcgcatg aaaaaatgat
     4801 attttaatga gcatcctgaa aaaatatcga tgcatttcga gcgaagatgg tggggatccc
     4861 tgaatgcgtt tttctcaccg actgttctta cttctgattc tcctgctgac gggagcacca
     4921 atactcgctc aagagccttc tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt
     4981 gtcagttaca cccgctggcc tgcgctatca gggccaccaa agttatgtat cttttcatct
     5041 tcacgtttta gtaccgccct acaagagaat gccgcgacat ctttgcctta tctgcctgtc
     5101 atcattcaca ctcaacaaga ggcgatgatt tcaggctgca atggttttta ttttggcaac
     5161 gagtcaccga cattccagat ggaattaacc gaacaatatc cgtcaaaggc gttattatta
     5221 attgccgaac aaaataccga gtgcattatt ggtagcgcct tttgtctgat catccacaat
     5281 aatgacgtca gatttgccgt aaacctggat gcctcatcgc gtagcggtgt aaaggtcaac
     5341 ccggatgtct taatgctcgc acggaagaaa aatgatggat aacgataatt ctcttaataa
     5401 gcgccccacg tttaaaagag cattacgcaa catcagtatg accagcatat ttatcactat
     5461 gatgctgatc tggttgctgc tttccgtgac ctcggtgctg accctgaaac agtacgcgca
     5521 aaaaaacctg gcactgacag cagcaacaat gacttacagt ctggaagcag ctgtcgtttt
     5581 tgccgatggc cctgcagcaa ctgaaacact ggcagcgctg ggccagcaag ggcattttca
     5641 actgcagaag tacgtgataa gcagcaaaat attctggcat cctggcatta cccccgtaag
     5701 gatccaggcg atactttcag caatttcata agccactggc tcttccctgc ccccatcatt
     5761 cagccgattc gtcacaatgg tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt
     5821 tcaatcagcc attttatctg gttttcgctc gccgtactga ccggttgtat tctgctggca
     5881 tcaggcatcg caattaccct cacccgccat ttgcacaatg gcctggtgga agcactgaaa
     5941 aatatcaccg atgtcgtaca tgatgtgcgt tccaaccgca atttttcccg acgagtttcg
     6001 gaagaacgta tcgctgagtt tcaccgcttc gctctcgact tcaacagtct gctggatgaa
     6061 atggaagagt ggcagcttcg tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta
     6121 catgacccat taaccgggct ggctaaccgc gcagcgtttc gtagcggcat caatacgttg
     6181 atgaacaatt ccgatgcccg aaaaacgtcg gcgttactat ttcttgatgg cgataatttc
     6241 aaatatatca atgatacctg gggtcatgcg acgggcgata gggtcttgat tgaaatcgca
     6301 aaacggttag ctgaatttgg cgggctgcga cataaagcat accgcctggg cggcgatgaa
     6361 ttcgctatgg tgctctatga tgtacagtcg gaatctgaag tgcagcagat atgctcagca
     6421 ctgacacaaa tctttaatct cccgtttgat cttcataatg gccatcagac caccatgaca
     6481 ttaagcattg gttacgcgat gaccattgag cacgcctctg cggaaaaatt acaagagctt
     6541 gccgatcaca atatgtatca ggccaaacac cagcgtgccg aaaagctggt gagataacaa
     6601 ggatatatcg atatgataaa gcacctggta gcacccctga ttttcacctc actaatactg
     6661 actggctgcc agtcccctca gggaaagttt actcctgagc aagtcgccgc tatgcaatct
     6721 tatggattta ctgaatccgc cggcgactgg tcgctgggct tatcagatgc cattctgttc
     6781 gcaaaaaatg actacaaatt gctcccggaa agccagcaac agatccaaac catggcagcc
     6841 aaattggcct cgacagggct aacacatgcc cgtatggatg gacacaccga taactatggt
     6901 gaagacagtt acaacgaagg cttatcattg aaacgggcga atgtcgtggc cgatgcatgg
     6961 gctattggtg gacaaattcc acgcagcaat ctcaccacac agggtttagg aaaaaaatat
     7021 cccatagcca gtaacaagac cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg
     7081 attgcaaccc cttaaaacac gagcaaacag acaaaaaaag ggccagccaa ttggccagcc
     7141 cttcttaaca ggatgtcgct taagcgaaat cttagttaag acgctctttg atacgagcag
     7201 ccttaccagt acgctcacgc aggtagtaca gtttagcttt acgaacagca ccacgacgtt
     7261 tgacagaaat gctgtcaact accggagagt gagtctggaa gacacgctca acgccttcgc
     7321 cgttggaaat tttacgaaca gtgaatgcag agtgcagacc gcggttacga atagcgataa
     7381 ccacgccctc gaatgcctgc agacgttttt tggaaccttc aacaacccat actttcactt
     7441 ccacggtatc acccggacgg aaggaaggta cgtcctgctt catctgctct tgttcaagtt
     7501 gcttaataat gttgctcata atttaatctc ttatcctggg taaactgatc tctcgggggc
     7561 ttacgccatc ccatcatgtt tatgttgctg ttgtgcgtgt tccgttttga actccgccag
     7621 caaccttgct tgctcttcag tcagagccag gttttccaga agttcaggtc ttctaagcca
     7681 ggtacggccc agcgactgtt tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag
     7741 taacactggc ggaacttcca tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc
     7801 cagcaatcct tcagcaaagg aatcttccgt tgccgaggct tcatgtccca gtactcccgg
     7861 aataaaccgg gaaacggagt caatcagcgt cattgctggt aactcaccac cactgagaac
     7921 gtaatcgccg attgaccatt cttcgtcaat ttcggtttgg atcacgcgct catctatacc
     7981 ttcgtagcga ccgcacacca gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc
     8041 ttgatcaagc ttgcgtccct gtggtgacag ataaatcacc tttgcgcctt cacccgccgc
     8101 ggcttttgct gcatgaatgg cgtcccgcaa gggttgcacc atcattaaca tccccggtcc
     8161 gccgccgtaa ggacgatcgt ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact
     8221 ccaactctgg atgctcagca ggccattttt aactgcccgg ccagttaccc cgtaatcggt
     8281 aattgcgcgg aacatttcag gaaacaggct aattatgcca atccacatag cgccgccttt
     8341 taccgtttat ccggtggttt aaaaaccagg atcccaatct acttcgattg aacgagtagt
     8401 gagatcgact ttcttgataa cctgcccatc gaggaacggt acgagacgtt ccttgatacc
     8461 aaacgcatct ttcaggtttg ccttaatgac gagaacgtca ttagatccgg tttccatcat
     8521 atcaacgact ttaccgagat cgtagccttc agtggttact acctggcagc ccatcaggtc
     8581 tttccagtag tagtccccct cttcaagctg aggcagctgc gatgaatcca cgacaatttc
     8641 acaattcgtc agcaggttcg ccgcatcacg atcgtcaacg cctttcagct tgatgatcat
     8701 gtcctgattg tggtgcttcc agctttccag ctggacttgc tgccactgac ccgccttctg
     8761 gataaaccag ggctgatagt caaaaatgct ttcggcgtct tcggtggaag aaaacactct
     8821 gagccaccca cgaataccgt aagacgaacc catttttccc aaaacgatgg gatcaacagg
     8881 tgcttgcgcg gtgagttgtt tgctcatcat gaccaccgtg acagattaag ctgctttgtt
     8941 tacttctttg atcagcgcag caacgcgatc agaaatagtt gcgccctggc caacccagtg
     9001 agcgatgcga tccagatcca ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa
     9061 gaaaccaacg cgctcgatga agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac
     9121 ctggtagaac ggacgctttt tagcgccgtg acgtgctaaa cgaatagtta ccataacatc
     9181 ctcttgtgtg aataaaacaa ccggacccca tcgaggaacg gagtccggtg tcatattaaa
     9241 agcccgaaaa ttttactgat ttctggagaa aatgcaatca gcagttaata actctgtcgc
     9301 ataaggtcat ttagcgacca ggaaatcccg gcggcatcat tcccttcatg cttctcatca
     9361 tcttcgccat tccgcctttc ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct
     9421 tcagaaggcg gttaacgtcc tgtacctgca tcccgcaacc ggcggcgata cgacgcttac
     9481 gcgaaccttt gatgatttct ggcttagcgc gctctttcat cgtcattgag ttgatgatgg
     9541 cttccatacg caccagcact ttatcgtcca tctgcgactt gacgttatcc gggatctgcc
     9601 ccatgcccgg cagtttgccc atcaagctgg ccatgccgcc catatttttc atctggcgca
     9661 gttgctcaag aaagtcgttg agatcgaagc cgtcgccttt ttttagcttg ctggctaatt
     9721 tctctgcctg cgcgcggtca actttgcttt cgatatcttc gatcagcgac agtacgtcgc
     9781 ccatgccgag aatacgcgac gcgatgcggt ccggatggaa cggctccagc gcctcagttt
     9841 tctcgccaac accgaggaac ttgatcggtt tgccagtgat gtgacgaata gagagcgccg
     9901 caccgccgcg ggcatcgccg tccactttgg tcaacactac gccggtaagc ggtaacgctt
     9961 cattgaatgc ttttgccgta ttggccgcat cctgaccggt catggcgtca accacaaaca
    10021 gggtttcaac cgggttaatc gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt
    10081 caacgtgcag acgaccagcg gtatccacca gcagcacgtc gtagaatttc agtttggctt
    10141 ctttcagcgc cgcgttaaca atatcaaccg gcttctgacc aacatcagaa gggaagaaat
    10201 caacgcccac ctgctctgcc agcgtctcaa gctgtttgat tgccgccggg cgatagacgt
    10261 cagcagaaac caccagcact ttcttcttgt gcttctcgcg caggaaatta ccgagtttac
    10321 caacgctggt tgttttaccg gcaccttgca ggcctgccat cagcacgacc gcaggcggtt
    10381 gcgcagccag attcagggtc tggttctctt cgcccatcgc cgcaaccagt tcgttacgga
    10441 cgattttgac gaactcctgc cccggcgtca ggctcttatt aacttcatga ccaaccgctt
    10501 tctcttttac gcgattgata aactcacgca ctaccggcag agcgacgtcc gcctccagca
    10561 gcgccatgcg cacttcgcgc agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc
    10621 cactgatatt gcgcagcgtg cgcgacaaac gatcggttaa attatcaaac attgtctctc
    10681 gcctggggtg gaaacggttg gccgcaatcg cgacacatca tcagtatttt gccgcagtat
    10741 aacatgaagg cgtctttgtt gttatgcaac ggttggagca gcgttcacct gacgctatac
    10801 tgcttctctt tcttattgct caaactgtcg acattactat gcccgttttt gctctgctcg
    10861 cgcttgtcgc ctactccgtc agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg
    10921 gcggctggcg gcgcatggct attatttctg cggtcattgc gctggtctgc cacgcaatcg
    10981 ctctggaagc ccgcatcctg cccgacggtg atagcggaca aaacctcagc ctgctgaacg
    11041 ttggttcatt ggtcagtttg gtgatctgta cggtaatgac cattgtggct tctcgcaatc
    11101 gtggctggtt gctgctaccc attgtctatg cctttgcgct tatcaacctg gcgctggcga
    11161 ccttcatgcc caatgaatac atcacccatc tggaagcaac gcctgggatg ctgatgcaca
    11221 ttggcttatc gctcttttcc tatgccacgc taattatcgc cgccctgtac gcgctgcaac
    11281 tggcgtggat tgattaccaa ctgaagaaca agaagctggc atttaaccaa gaaatgccgc
    11341 cattgatgag tatcgagcgt aaaatgttcc acatcacgca gattggcgtg gtgctgctaa
    11401 cgctcacgct ttgcactggc ctgttctaca tgcacaacct atttagcatg gaaaatatcg
    11461 acaaggctgt gctctctatc gtggcgtggt ttgtctatat tgtgctgctg tggggacatt
    11521 accatgaagg atggcgtgga cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc
    11581 tgacactggc ctacttcggc agccgaattg tccagcagtt aatcagctaa acccagaaaa
    11641 ggagtttccc ctggaacaca tttctactac tacgttgatc attattctga tcatcatggt
    11701 ggtcatttca gcctattttt ccgggtccga aaccggaatg atgaccctca accgctatcg
    11761 tcttcgacat atggcgaaac agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg
    11821 taagccagac cgcctgataa gcctggtgtt aatcggcaat aacctggtca atattcttgc
    11881 ctccgcgctc ggcactattg tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc
    11941 gactggtgtg ctgacttttg tcgtactggt ttttgctgag gtattgccga aaaccattgc
    12001 cgcgctgtac ccggaaaaag tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat
    12061 tttgatgatg ccgctggtct ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat
    12121 gggtatcaaa accgatatcg tggttagcgg ctctttgagc aaagaagagt tgcgcactat
    12181 cgtgcatgaa tcgcgctcac aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct
    12241 cgatctggaa aaaatgaccg ttgatgacat catggtaccg cgcagtgaaa ttatcggtat
    12301 tgatatcaac gatgactgga aatctattct gcgccaactc tcccactcac ctcacgggcg
    12361 catcgtgctt taccgtgatt cgctggacga cgccatcagt atgctgcgag tacgtgaagc
    12421 ctggcggttg atgtcggaga aaaaagagtt caccaaagaa accatgctgc gcgccgcgga
    12481 cgagatctat tttgtgcctg aaggtacgcc gctcagcacg cagttggtaa agtttcagcg
    12541 caacaaaaag aaagtcggcc tggtcgtcaa cgagtatgga gacattcagg ggctggtgac
    12601 ggttgaagat attctggaag agattgttgg cgatttcact acgtcgatgt cgccaacact
    12661 tgccgaagag gtcacgccgc aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt
    12721 gcgggaaatc aacaaagcct ttaactggca tctaccggaa gatgatgccc gcacggttaa
    12781 tggcgtcatt cttgaggcac tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg
    12841 caagtacgat atcgatattc tcgacgtaca ggacaatatg attaagcagg taaaagtttt
    12901 tcctgtgaaa ccgctgcgcg agagtgtggc ggagtaacga aaacggccct gcattcgcat
    12961 gcagggccgt gaattattac gaaaacagaa attacgcttt tgctttcgct acagttacca
    13021 tcgccgcacg aatcgtacga ccattcagcg tataaccctt ctgcataatg cccagtacgt
    13081 tacctggcgc aacgtcatca gattccacca ttgcgatagc ctgatgcaca ttcgggtcca
    13141 gcggcacgtt agtttcggcg atcacttcaa caccaaactt acgcacaaca tccagcatcg
    13201 acttcagcgt cagctcaatg ccttcaacca tcgcagacat atccgggtta gctttatcag
    13261 ccacttccag cgcacgatcc aggctatcaa tcaccggcag caattcgttg atgaatttct
    13321 ccagcgcgaa tttgtgggct ttttcaatat ccagttcagt acgacgacgc aggttttcca
    13381 tttcggcttt tacgcgcaaa ataccgtcac gttcacgggt ctgggcttca gccagctgag
    13441 cttcgagatt cgcaattttt tcatcgcgcg gatccacctg ctcagcagaa gcttctggct
    13501 caactgcctc aatctcttcg tgctgatcca tgataatttc ttccggggct tgcccctcag
    13561 gcgttttctg ttctttacta ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct
    13621 aacttcgctt attatgggga tcagtttcag ggtttcaagg gaagcactca cattgtcatc
    13681 aatcttcgca acaaggacct cggaaaaatg aataatcatt tcaagtgtat tggcattgtg
    13741 ggacacccac ggcaccctac tgcactgaca acacatgaaa tgctctaccg ctggctgtgc
    13801 acaaaaggtt acgaggtcat cgttgagcaa caaatcgctc acgaactgca actgaagaat
    13861 gtgaaaactg gcacgctcgc ggagattggg caactggctg atctcgcggt agtcgttggt
    13921 ggcgacggta atatgctggg cgcggcgcgc acgctcgccc gttacgatat taaagttatt
    13981 ggaatcaacc gtggcaacct gggtttcctg actgaccttg accccgataa cgcccagcaa
    14041 caattagccg atgtgctgga aggtcactac atcagcgaga aacgtttttt gctggaagcg
    14101 caagtctgtc agcaagattg ccaaaaacgc atcagcaccg cgattaacga agtggtactt
    14161 catcctggca aagtggcgca tatgatagag ttcgaagtgt atatcgacga gatctttgcg
    14221 ttttctcagc gatctgatgg gctaattatt tcgacgccaa caggctccac cgcctattcc
    14281 ctctctgcgg gcggtcctat tctgaccccc tctctggatg cgattaccct ggtgcccatg
    14341 ttcccgcata cgttgtcagc acgaccactg gtcataaaca gcagcagcac gatccgtctg
    14401 cgtttttcgc atcgccgtaa cgacctggaa atcagttgcg acagccagat agcactgccg
    14461 attcaggaag gtgaagatgt cctgattcgt cgctgtgatt accatctgaa tctgattcat
    14521 ccgaaagatt acagttattt caatacatta agcaccaagc tcggctggtc aaaaaaatta
    14581 ttctaatttt acgccagcct ctttactgta tataaaacca gtttatactg tacacaataa
    14641 cagtaatggt ttttcataca ggaaaacgac tatgttggca caactgacca tcagcaactt
    14701 tgctatcgtt cgtgagcttg agattgattt tcatagcggc atgaccgtaa taactggcga
    14761 gaccggtgcg ggtaaatcta ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc
    14821 tgaagccgac atggtgcgta ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct
    14881 gaaagatacg ccagcggccc tgcgctggct ggaagaaaac cagcttgaag acgggcatga
    14941 atgtttgctt cgtcgcgtga tcagcagtga tggtcgctcc cgtggtttca tcaacggtac
    15001 agctgttcct ctgtcacaac tgcgcgaact gggtcagttg ctgattcaga tccatggtca
    15061 gcacgctcat caattactca ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc
    15121 caatgaaacc tctctacttc aggaaatgac cgcacgttat cagttgtggc atcaaagctg
    15181 ccgtgacctc gcgcatcatc aacagttaag tcaggaacgc gccgcccgtg cggaactgct
    15241 gcaataccaa ttaaaagaac ttaacgaatt taatccgcag cccggagagt ttgagcaaat
    15301 cgacgaagag tacaaacgtc tggcgaacag cggtcaattg ctgaccacca gccagaatgc
    15361 attggcatta atggccgacg gtgaagacgc aaacctgcaa agtcagcttt acacggctaa
    15421 acaactggtg agcgaattga ttggcatgga cagcaaactg tccggcgtac ttgatatgct
    15481 ggaagaagct accatccaga ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg
    15541 tctggatctc gatcccaacc gactgtttga acttgaacag cgcatctcaa aacagatttc
    15601 gctggcacgt aaacatcacg tcagccctga ggcattacca cagtattacc agtcgctact
    15661 ggaagaacag cagcaactgg acgatcaggc cgactcacaa gaaacgctcg cgctggcggt
    15721 aacgaaacat catcagcagg cactggaaac cgcgcgcgca ttacaccaac aacgccagca
    15781 atatgcagaa gaacttgcac agctgatcac cgacagtatg catgcgctct caatgccgca
    15841 tgggcagttt acgattgatg tcaaatatga cgagcatcac ctgggcgctg acggtgccga
    15901 tcgtattgag tttcgggtaa ccaccaaccc aggtcagcca atgcagccta ttgccaaagt
    15961 cgcatccggt ggtgaattgt cccgcatcgc actggcaatc caggtcatca cggcgcgtaa
    16021 aatggaaacc ccggcactga tttttgatga agtggatgta gggattagcg gtccaacagc
    16081 ggcagttgtc ggcaaactgc tgcgtcaact cggcgaatca actcaggtga tgtgtgttac
    16141 ccacctgcca caagtcgcgg gatgtggtca tcaacactat tttgtcagca aagaaaccga
    16201 tggtgcgatg acagaaacac atatgcaatc tctggataaa aaagcgcggt tacaagagct
    16261 ggcgcgcctg cttggtggca gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact
    16321 gcttgcagcg taaacttttt tcctgcttca cggtcagagt aaacagcaaa acgccgtaag
    16381 accggaaagc aaaaggtttt aaagtgatga aaggtctatt atcatcggca tattacagat
    16441 gagccacgta ctgctcgggc ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga
    16501 ctgctgcagc agcagtacta ttgatgttaa ccgcaggctg ttccactctg gagcgagtgg
    16561 tttaccgtcc tgacatcaac caggggaact atctgaccgc taacgacgta tccaaaatac
    16621 gtgttggcat gacgcaacaa caagttgcgt acgcattggg tacaccgctg atgtccgatc
    16681 catttggtac gaatacctgg ttctatgtct tccgccagca accaggtcat gaaggtgtaa
    16741 cgcagcaaac gctgacgctg acctttaaca gtagcggtgt gttgaccaat attgataaca
    16801 aacctgcgct gagtggtaac taataataaa gttgctctca aagacgttaa aaaaggtgct
    16861 caatgagcac cttttttctg tctgttattt attcgctgat ttttctgctc gttgcctgcg
    16921 aagctctttc ggatcggcaa tgagagggcg ataaatctcc acccgatcgc catcatgcac
    16981 actatcgctt agttttgccg gacggctgta aatgccgact ttgtttttag ttaaatcgat
    17041 atcggtacgc aattccagca agccactggc gcgaatagct tcttcaaccg tcgcgccctc
    17101 ctgcagcgtc actcgctgca ggtattgctt ctcaggtagc gcataagcca cctcaacggc
    17161 aattttacct agcactgtaa acctctttcg cacgaaccgt aaaagcctgg accatattag
    17221 ccgccagctc tttaaacacg cgaccaaagg cgagttcaat caacttattg gtaaactcaa
    17281 agtcgagatg aaactcgata cgacacgcct cctggctcag cggcgtaaac ttccatccac
    17341 caatcaattt cttgaacggc ccatccacca gattcataag aatactttgg ttactggtca
    17401 actggttgcg ggtagtaaac gttttgctga tcccagcctt agagacatct accgccgcag
    17461 tcatctgccc aggagtggac tccagaatcc gacttccggt acaacctggc aaaaactgag
    17521 gataagactg aacgtcattc actaactgat acatttgctc cgcgctgtag ggtaccagtg
    17581 cggtccggct aatctgaggc ataacaattt ccatcaacaa aaatccaaca aataatatca
    17641 tttatcctgc tgtaaaaaaa acgctatccc ggcgctgggt aacatcgggt tcatgctaag
    17701 atagagcctt gtcccccgca ggattgatat ggggtatttt cgatttcaga ttaccgatga
    17761 ttcacgacgc ttatgacgaa gaaaaaagca cataaacctg gttcagcgac catcgcgctt
    17821 aacaagcgcg cccgtcacga atactttatc gaagaagagt tcgaagcggg acttgccctg
    17881 caaggctggg aagttaaatc cctgcgcgca ggaaaagcca atatcagcga cagctacgtc
    17941 cttctgcgtg acggagaggc atttctgttt ggcgctaaca tcacgccaat ggccgtggcc
    18001 tccacgcatg tggtgtgcga tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa
    18061 ctggactcat tgtacggtcg cgtcaatcga gaaggctata ccgtagtggc gctctccctg
    18121 tactggaaaa atgcctggtg caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac
    18181 gataaacgtt cagatatcaa agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa
    18241 aacgcccacc gttaaacctg cactccaatt attgaccagt tcctcaccgc gcctccctct
    18301 ccggcggcgc gaatgaacat cttattggct atcacatccg acacaaatgt tgccatccca
    18361 ttgcttaatc gaataaaaat caggctacat gggtgctaaa tctttaacga taacgccatt
    18421 gaggctggtc atggcgctca taaatctggt atacttacct ttacacattg gggctgattc
    18481 tggattcgac gggatttgcg aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa
    18541 aagccgcaaa aaatagtcgc aaacgacgaa aactacgctt tagcagctta ataacctgct
    18601 tagagccctc tctccctagc ctccgctctt aggacgggga tcaagagagg tcaaacccaa
    18661 aagagatcgc gtggaagccc tgcctggggt tgaagcgtta aaacttaatc aggctagttt
    18721 gttagtggcg tgtccgtccg cagctggcaa gcgaatgtaa agactgacta agcatgtagt
    18781 accgaggatg taggaatttc ggacgcgggt tcaactcccg ccagctccac caaaattctc
    18841 catcggtgat taccagagtc atccgatgaa gtcctaagag cccgcacggc gcaagccctg
    18901 cgggcttttt tgtgctccga atttgtcccg cgaagtctga tgccaactaa ttaaatccga
    18961 tccttttagg caccttgtta ggcacttcat aaagctatat tgtctttgag gtgcctaaaa
    19021 ctatggaaac ccggcaatgg caagacaaac caaacctcta tccgttaagg aaatcgaatc
    19081 tgctaaaccc aaggaagcgg actacgttct ctatgatggc gatggccttg agctactcat
    19141 taaatccagc gggagtaaaa tctggcagtt tcgctacatt cgccctgtca ccaagacacg
    19201 agcaaagaaa agcataggcc cttacccgtc agttaccctt gccgatgcca gaaactaccg
    19261 tgcagagtcc cgctcactcc tagctaaaca gattgatccg caggaacatc agcaagaaca
    19321 attgcgtagt tcgctggaag ccaaaaccaa tactttccag ctcgtagctg aacgttggtg
    19381 gaatgtgaag aaagccagtg tgacagagga ctatgcggaa gatatctggc gctctcttga
    19441 aagagatgtc tttcctgcga ttggcgacgt tagcgttaca gatattaaag ctcatacact
    19501 ggttcaggcc gtacaaccgg ttcaggccag aggagcactg gaaacggttc gtcgcctgtg
    19561 tcaacgtatt aatgaggtca tgatctatgc ccagaacaca gggctgattg atgctgttcc
    19621 cagcgttaat atcggtaaag ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg
    19681 accggatcag ctatctcaac tgatgcagac aatgcgaaca gccagcatta gcctttccac
    19741 acgctgcctg ttcatgtggc aacttcttac tattacccgc cctgccgaag cggctgaagc
    19801 tcgctgggaa gaagtagaca tagaagcgca agagtggaag attcccgcag cacgcatgaa
    19861 aatgaaccgc gaccatactg ttccattgtc agatgaagca atagctgtgc tggagatgat
    19921 gaaaccgcta agcggcaacc gagaatttat ctttcctagc cgtatcaagc ccaaccagcc
    19981 tatgaacagt cagactgtta acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt
    20041 ttcgcatggt ctgagatcaa ttgccagtac ggctcttaat gaacaaggct ttccgcctga
    20101 tgccattgag gcagcattgg cccatgtgga taagaatgag gttcgtcgtg cttataaccg
    20161 cagcgattac ctggaacagc gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc
    20221 agcggatcgt ggaagcatga ttgaaggtgg gataaaagga atgaagctag ttgggtgact
    20281 aaatagctgc gcggcatagt gtcccacttt cagccagccc ttgcaggtta agccaccaga
    20341 cagtaaacgg cgggtgtcgc gttattaggc gcagctattt agtagtaata ttaggattgg
    20401 tgattctgca ctccagaagt tcggtaatgc tgccaactta ct
//
LOCUS       DAJTQQ010000070        20298 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000070 DAJTQQ010000000
VERSION     DAJTQQ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20298
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.070"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(60..911)
                     /gene="manZ"
                     /locus_tag="OO309_002756"
     CDS             complement(60..911)
                     /gene="manZ"
                     /locus_tag="OO309_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR5847846.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(924..1724)
                     /gene="manY"
                     /locus_tag="OO309_002757"
     CDS             complement(924..1724)
                     /gene="manY"
                     /locus_tag="OO309_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR5847847.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(1787..2758)
                     /gene="manX"
                     /locus_tag="OO309_002758"
     CDS             complement(1787..2758)
                     /gene="manX"
                     /locus_tag="OO309_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR5847848.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3221..4771
                     /gene="yoaE"
                     /locus_tag="OO309_002759"
     CDS             3221..4771
                     /gene="yoaE"
                     /locus_tag="OO309_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR5847849.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(4775..6373)
                     /locus_tag="OO309_002760"
     CDS             complement(4775..6373)
                     /locus_tag="OO309_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5847850.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6504..7868)
                     /gene="sdaA"
                     /locus_tag="OO309_002761"
     CDS             complement(6504..7868)
                     /gene="sdaA"
                     /locus_tag="OO309_002761"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5847851.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8052..8630)
                     /locus_tag="OO309_002762"
     CDS             complement(8052..8630)
                     /locus_tag="OO309_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR5847852.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            complement(8634..9995)
                     /gene="pabB"
                     /locus_tag="OO309_002763"
     CDS             complement(8634..9995)
                     /gene="pabB"
                     /locus_tag="OO309_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR5847853.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10069..10248
                     /locus_tag="OO309_002764"
     CDS             10069..10248
                     /locus_tag="OO309_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR5847854.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10368..10727)
                     /locus_tag="OO309_002765"
     CDS             complement(10368..10727)
                     /locus_tag="OO309_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR5847855.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11089..11433)
                     /locus_tag="OO309_002766"
     CDS             complement(11089..11433)
                     /locus_tag="OO309_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5847856.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            11565..13475
                     /locus_tag="OO309_002767"
     CDS             11565..13475
                     /locus_tag="OO309_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR5847857.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13533..14228
                     /gene="tsaB"
                     /locus_tag="OO309_002768"
     CDS             13533..14228
                     /gene="tsaB"
                     /locus_tag="OO309_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR5847858.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14268..14849
                     /gene="yeaY"
                     /locus_tag="OO309_002769"
     CDS             14268..14849
                     /gene="yeaY"
                     /locus_tag="OO309_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR5847859.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15055..16740
                     /gene="fadD"
                     /locus_tag="OO309_002770"
     CDS             15055..16740
                     /gene="fadD"
                     /locus_tag="OO309_002770"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR5847860.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            16822..17937
                     /gene="rnd"
                     /locus_tag="OO309_002771"
     CDS             16822..17937
                     /gene="rnd"
                     /locus_tag="OO309_002771"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR5847861.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(17991..18956)
                     /gene="yeaX"
                     /locus_tag="OO309_002772"
     CDS             complement(17991..18956)
                     /gene="yeaX"
                     /locus_tag="OO309_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR5847862.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIVGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19012..19629)
                     /gene="yeaW"
                     /locus_tag="OO309_002773"
                     /pseudo
     CDS             complement(19012..19629)
                     /gene="yeaW"
                     /locus_tag="OO309_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(19971..>20298)
                     /locus_tag="OO309_002774"
     CDS             complement(19971..>20298)
                     /locus_tag="OO309_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5847863.1"
                     /translation="NKNSQPDRSTLKTKLIAIVELLEDKELHSAIELFRKKGFEALMP
                     EIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRKKILSKYGIYEQEGLVAPSQEPQ
                     DVKKAV"
BASE COUNT         4990 a         5060 c         5249 g         4999 t
ORIGIN      
        1 gtcattaatc ctccagataa aaaaacgggg ccaaaaggcc ccggtagtgt acgacagtct
       61 tacagtccca gcaggccgca agcgtaacca gcgataccga tgacgaagaa gccaacgatg
      121 atccacagcg ggttaacttt tttgcgcagc agccacatac aagcaaatgt cagcagcagt
      181 ggtacaaggc ctggcattaa ctggtcaaga atagtttgca cggtggtgac acgggtctgt
      241 ccatcctgcc cggtgatggt cgaaaccacc agcgggatgt tgacatgtgt ccacttgtta
      301 accaatgccc ccatgacaaa caggccgagg atagacgccc cttccgtcag tttttgcagg
      361 aagccaccac ccatatcttt aacgatatcg atacctttgg agtaaccata cgctacgccg
      421 tagtaacggg ttgccagacg caccaggtta aacaggatga agaacagcag cggacctaac
      481 aggctgccgc tcatcgcgat accggcaccc agtgctgcaa ataccggacg tacggttccc
      541 cagaagatcg ggtcgcctac accagccagc ggccccatca aaccgacttt gataccgttg
      601 atagcaccgt catcgatctc tgcgccatta gcacgctgtt cttccagcgc cagggttacg
      661 ccgagaatcg gtgcagccac gaacggctgg gtgttaaaga actccaggtg acggcgaata
      721 gcttgtttac gagcttcgtt gttctcaggg tagaggcgac gaattgccgg taccatagag
      781 aagcagaaac ccagtgcctg catacgttcg aagttccatg aaccctggaa gaggttagaa
      841 cgcaggaata cgccacgaat atcactttga gtgagttttt tctcggtggt agtttgagtt
      901 gtatcaacca tttcgctcac ctgttagtcc agttcgttat cgagatcgtt gttaccagct
      961 gcctgagcag gcgcaccggc tacgcggttg tatttcgggc taagttggat gtagagcact
     1021 gccataacag taccaatcac acccagagca accaggttaa agttggtgaa tgctgcggtt
     1081 acgaagccga ggtagaagaa cggcatcagg tagccagcac gcatcatgtt gataaccatc
     1141 gcataaccaa ccaccacgat catgcccccg gcgatattca gaccattggt caccacttcc
     1201 ggaatcgcat tcagcatgtt ctgtacttcg ctggtaccaa cagacagcgc aacgatgacg
     1261 gccggaatag ccacacgcat tgcttgcagg aacagagaag aaacgtggat ccaggaaatc
     1321 gctgtcaggt tgccgttatc agcagcctta tccgcagcgt gctggaaagc aacggtaata
     1381 gtacgaacga tgatggtcag tacctggccc gcagcggcca gagggattgc cagtgcgata
     1441 cctgcaccaa tgctctgatg acctgcgata accagaatgg tagaaatgat agaagccaga
     1501 gcggcgtcag gcgcaactgc agcaccgatg ttcatccagc ccagcgcgat catttccagc
     1561 gtaccaccga taataatacc ggttttcata tccccaagaa cgatacccac cagggtacac
     1621 gcgattagcg gacggtgaaa ctgaaattca tcgaggattg atcccatacc tgcgatacag
     1681 gctacgataa ataccagcac aatttgaaga gtggtaatct ccattgtact tctcctgttt
     1741 acgtaagact taagtgtgaa aactgaatga taatcaacac aatacgttac ttatcgattt
     1801 tgctgatcag atccatcatt ttcagtttcg gatcggtgga aaccttacgg acttccagct
     1861 caataccgcg tgcattcagt tttttgaacg cctcgatatc tttttcatca accgaaaccg
     1921 cgttattcac ctgggtttta ccctgacgga atgccatacc accgacgtta acagaggtga
     1981 ttttcacgcc gccttcaacg agacgctcta catctgttgg gttggtaaat aacagcatta
     2041 cgcgttcgcc agcatatttc gggttgttgt agacgcgaat cattttggca acatcaacta
     2101 cgtgtgctgt tacgcccgga ggtgcaacct gggtgagcag tgtcttacga acggtatccg
     2161 cagccacttc atcactaaca acaataatac gggagacatt ggtttctttg gtccagcggg
     2221 tggcgacctg accgtgaatc agacggtcgt cgatacgcgc aaggccaata accatgtagt
     2281 cgtttggccc cattggtttt gccggagttg gagccgcttt tggtgctgcg gcagcgggtg
     2341 ctggcgcggc tttttcaacc ggtttggctt tcagtgcttt cacgccttca cggcctgttt
     2401 ctactgccag cgcaaccagt tcatcaaagc tcgggtcatc atcacgggcc attaacgttt
     2461 ccacgagcat tggaatgtta acgcctgcaa tgacttcata atgctctttg tcgacgacaa
     2521 tgcggctggc agcattgaac gggctgcctc cccatgtatc aacgagaaac agcacgcctt
     2581 tagtggtgtc gagttttgcc aactgagcgt tgtatttttc aatcagcgtt tcggcatttt
     2641 cacctggaac gaaatcgatc cagccgacgt tttcctgctc gcctaacagc atttctgccg
     2701 ttttaagcaa ctgctctgca gcccaaccat gtgtgcctat aacaatagca atggtcactt
     2761 gctacctcct ttattatcgt taacacctca acgtgccaga tgtatttttg aatcgtcgtc
     2821 cacaatcgaa tcgattcaga taagggctac aaccaaaaac ccctgcgttt cgcgatttat
     2881 tttagatatc gaaaaaatta ttttatgtga tgaagatccg taatttaact ttcgattatc
     2941 agaaatttcg aaaggtaaaa tatccttgtc acattcgttt gcaaaggaag gtaaatcttt
     3001 gccaaatcag aggcgtctct gatatgttta actcccgttt aacaaccatg gagtataggg
     3061 cagtagcccg cagtatggat cgtcaccgac gtcatttcag catcaggcct tttaacgcct
     3121 gcctttctgg cactctatgc cgcacctttc gtttgcattt tgtcgttacg cctgcattat
     3181 ttctggcgtc gaatagctat tccttaagca ggagcttgtc atggaattct taatggaccc
     3241 ctcaatttgg gcggggctac tcacgcttgt tgttctcgaa attgtgctgg gtatcgataa
     3301 cctggtcttc atcgccattc ttgctgacaa actgccgcca aaacaacgcg ataaagcgcg
     3361 tttgctgggg ttatcactgg cgctgattat gcgtctgggg ctgctgtcgc tgatttcatg
     3421 gatggtcacg ctgaccaaac cgctatttac cgtcatggat ttctccttct ccggacgcga
     3481 tctgattatg ttgttcgggg ggatattctt gctgttcaaa gcaacaaccg aactgcatga
     3541 acggctggaa aaccgcgatc atgattccgg ccacggtaaa ggctacgcca gtttctgggt
     3601 ggtcgtcaca cagattgtca tccttgacgc cgtcttctcg ttggatgcgg taattactgc
     3661 agtagggatg gttaaccatc tgccggtgat gatggcggcg gtagtgattg cgatggcggt
     3721 tatgttgctg gcatcgaaac cgctgacgcg attcgttaac cagcacccca cggtggtggt
     3781 gctctgtctg agcttcctgt taatgattgg tctgagtctg gtggcagaag gtttcggttt
     3841 ccacattccg aaaggttacc tgtatgccgc gattggcttc tcgatcatta tcgaagtgtt
     3901 taaccagatt gcgcgtcgca actttattcg tcaccagtcg actttgccgc tgcgagcgcg
     3961 tactgccgat gccatcctgc gtttgatggg cgggaaacgt caggccaatg ttcagcacga
     4021 tgccgataac ccgatgccga tcccggaagg tgcatttgcc gaagaagaac ggtacatgat
     4081 taacggcgta ctgacgctgg cgtcgcgttc tctgcgcggg atcatgacgc cgcgcggtga
     4141 aataagctgg gttgacgcta atctcggggt cgatgaaatc cgcgagcaac tgctctcttc
     4201 accgcacagt ctgttcccgg tatgtcgcgg tgaactggat gaaatcatcg gtatcgtacg
     4261 tgctaaagaa ctgctggtgg cgctggaaga gggcgttgat gtggcggcga ttgcttcggc
     4321 gtctccggcg attatcgtcc cggaaaccct cgatccgatc aacctgctgg gcgtgctgcg
     4381 tcgcgctcgc gggagctttg ttatcgtgac caacgagttt ggtgtggtac aaggcttggt
     4441 cacgccgctg gatgtgctgg aagccattgc gggtgaattc ccggacgctg acgaaacgcc
     4501 ggaaatcatt accgatggta acggctggct ggtaaaaggc ggtacagatt tgcatgcctt
     4561 gcagcaggcg cttgatgttg agcaccttgc cgatgacgat gatatcgcga cggtcgcggg
     4621 cctcgtgatc tcggcaaatg gtcacattcc ccgtgtgggc gatgtgattg atgtagggcc
     4681 actgcatatc accatctttg aagccaatga ttatcgtgtt gatctggttc gcattgttaa
     4741 agagcaaccg gcgcacgatg aagatgagta agcgttaacg taacgacata atgggcgtga
     4801 tatgtccatt atgccgggcg ggcggcggtt ggctgcccgc cagccatttg ggaaaatcac
     4861 gtagcggcat cggctgtgcg tacaaatacc cttgcaaaat atgcacgcca tgacggcgca
     4921 aatacttcgc ctgttcttgc gtttccacgc cctccgccac cagttcaata ttcagtcttt
     4981 gccccagcgc gatgatgata tcggtcaccg tcgagttaac cgcgtcagaa cctatagctg
     5041 cggtaaatga cttatcaatt ttcagcacgt caggacgtaa tgtttcaagc caggaaaacg
     5101 agctgttgcc ggtgccgaag tcatcaatcg ccagtttgac gtttttacga tgcagctcgc
     5161 gggcaatccg ataatcaaca tccagtaagg catcgcgttc ggtgatttcg aggatcagtt
     5221 gctgaatcgg gtgagcgcta aaccagtact gattgagatc ttttatcagc acaccacggc
     5281 gaaaatggct gggtgcgacg ttaatgccaa catgaaactg actactcatc gggaaaacat
     5341 ggtgctgacg aatggtttct gccatcacat agtgggtcag tggcacaatt aaatgatgtt
     5401 cttccgcgat aggaataaac acatccggtg aaatccagcc ctgacgcgga ttattccagc
     5461 gcagcaaaat ctctacacca atacattgct ggctgcgcgc attaagcaaa ggctgacaga
     5521 acaattcgaa ctcatgttgc gccaggccca gattaatttc gcgggaaaag ctcatccggt
     5581 aagcggtcgc cagccaggcg atatagccca ccagtaaact tagcagcacc gccagcggta
     5641 attgtgttgg aagatagtgc catgccagcg ccgtagcccc cggaccattt acgttaatgg
     5701 taaaaggaaa gctttgcgaa gaaacctgat agttttcatt gtcttcaggt tgcggaaggg
     5761 aatctaccag cccattacca tagagcaaat gccgtttgtc caccgtcagg ctggcgctac
     5821 tgatttgcgg cagttgtggc tcaaggagca ttgccgtcag taagtcgatg ttaatcatct
     5881 ctatgacccc agcattgcta ctgcccgctg caggcgtcca ttgaatcaaa accggactgc
     5941 ctttaatcag ggcacggtcg atcgtcaggc gtaaaagtgg ttgcggtgca ggaagttcag
     6001 ccagaatgtc cacgacgggc acattgcgat aaccaaaaat gctggagcaa taaagtgtgc
     6061 cgtcttgcac caggccaatg gatcgcacag tttggagttt tgccgcctgt ttacgtaatg
     6121 gcaaatgggc gacagagcag ggcagaccaa tcagcggaag caagactttg ctacctgcct
     6181 gtaggggaag cagtacttta tctaattcct ctacagcgtg attggcgaat tgtactaccc
     6241 gttgttgatt taagttacgc tgtgaaataa atcgcacgct cagggtcgaa gcgagcgtaa
     6301 cgagtgcgca aatcgtacaa acaatcattc gattacggcg ataggtttta atgatccgtt
     6361 gtgctttttg catgcgggcc accctgtaag cgtatggcga caaacgcctg acgcaacagt
     6421 ggaagtgtag tggggaatat tacagcagac gagaaagcgg gtataaattc gcccatccgt
     6481 tgcagatggg cgagtaagaa gtattagtca cactggactt tgattgccag accaccgcgt
     6541 gaggtttcgc ggtatttggc gttcatgtcc ttaccggttt cgtacatcgt ttcgatgacc
     6601 ttatccagcg agacgcgcgg tgcactggtg cggcgcagag ccatccgtgc ggcgttaatc
     6661 gccttcacag aggcaatggc attacgctca atgcacggca cctgaacctg ccctgcaacc
     6721 gggtcgcagg ttaaaccaag gttgtgttcc atgccaattt ccgccgccac gcaaacctgt
     6781 tccgggctac cgcccagcag ttctgcaaga cccgcagcag ccattgaaca ggcaacaccc
     6841 acttcgccct ggcaaccaac ttccgcaccg gaaatagagg cgttcatttt atacaatgca
     6901 ccaatcgcgc ccgctgccat aaagtaacgg gtatagatgt ctgggctgac cgattcaata
     6961 aagtggtcat agtaagccag cactgccgga acgataccgc aggcaccgtt ggttggcgca
     7021 gttaccacac gaccaccggc ggcgttttct tcgttaactg ccagcgcaaa catgtttacc
     7081 cagtcaatga cattcatcgg atcgttagac agtttatcgc tggaaactaa catccggcgc
     7141 agggcagacg cacgacgtgg cacgcgcagc gggcctggca gtacaccttc ggtgttcatc
     7201 ccgcgatcga tacatgcctg catggtttgc cagacatgcg cgaaatactc gtcgatctct
     7261 ttcttgctgt gcagcgccag ttcgttctgc atagcgagac cagacagcga atagccggtt
     7321 tcattacagt acgcgagcag ttcggtggca gatttgaacg gatacggcac gctcacttcg
     7381 ttggcagcat cctgaccaaa gtgttcttca tcgacgataa aaccgccgcc gatggaataa
     7441 taagttttgc tgtagacgac ttcatcgccg ttataggcgt ggatttgcat accgttttca
     7501 tgcagcggca ggttgccgtt atgaaaacgc atcccgttgt cgcgcgggaa atccacttca
     7561 tgccgtccct gtgccagcag cagacgttcg cgctctttta cgtcgcgaat aaaaccggga
     7621 atactgtcga tatccacggt ggcaggttcg ttacctgcaa gacccataat aatggcgata
     7681 tcggtgtggt ggcctttacc cgtcagcgac agtgaaccat aaacgtccac ggcaacgcga
     7741 gtaacgctat ccagtaagcc tttttcgacc agatcatcga cgaactgttt acccgccttc
     7801 ataggcccta cggtatggga agatgaggga ccaatcccca ccttaaacat gtcgaataga
     7861 ctaatcacga taatactcct gacaaggtga ctggacttcc agtaacgaac taataacagc
     7921 gcatagtgta agagggaacg cggcgactgg cttaactatt cacatgaatt aaactaatca
     7981 aaaaccgcga tttaactaat attgcgatga ttgtctcaag tagtgttttg tagatgtaaa
     8041 gataagtata gtcagggggt cacaccaatt tgcagcgcca gctcacgaat tatgcctgcg
     8101 gtcattcccc atacaaaata ctgttcgtac caggacagcc atacccgatg tgaatcgcca
     8161 cggcggtaga tatctaaagg gtgataacga cccagatgta atgcctgggc gagcggcatt
     8221 tcaaacaccg ccgagacttc atcttcactg gcgcgatacg gcagatcggg cgggataatg
     8281 ccgaccactg gggttacttg atagccagtt acgctatcga cgggcggcag cacgccgata
     8341 acttcaacgg cggaaggcgg tatagcgacc tcttcttcag cttcgcgcag cgcggcggcg
     8401 ataactgatg cgtccgtgtc atcgactgca cctccaggga atgccacttg tccagcgtgt
     8461 ttacgcagat gaattgaacg ctgagtcagc aacaaccccg gttgcggtcg acggacgatg
     8521 gggattaaca cggcagcctg acgatgattt aggggttccc ggttaatttg cgggcgcaaa
     8581 agttgaaagc gagataaaaa atcatcaagc gtcaggctac ggtattccac gtcttacttc
     8641 tccagttggc gtaatatctt attaacttta tcaaaagttt cctgatattc cgcttcttcc
     8701 tggctatcgg cgacaattcc accgcccgca gagcagtata tttgtccgtt aatggcagtc
     8761 agcgtgcgga tagtaatgct ggtatccatg ttgccgcaaa agctcaaata gccaatgctg
     8821 ccgcaccagg cattacgtcg ctgcggttcc agttcgtcga taatttccat agcccgtact
     8881 ttcggagccc cggttattga gccaccagga aaagctgcgc gcagcagatc gctggcgtgt
     8941 aactgttctg gtagtcgcgc cgttatagtg ctgaccagat gatgcacggc agggaagggt
     9001 tccaccacga agagctctgg tacttttacc gaaccggcta cggcaacacg accgatatca
     9061 ttacgcatta aatcgacaat catcagattt tcggcacgat ctttcgctga gttcgccagt
     9121 ttttctgctt gtttgctatc ttcctgagga tcgggcaggc gtggtagcgt gcctttaatc
     9181 gggcgagtct ggatttcact attatcacaa agaataaacc gctctggcga aaggcttaaa
     9241 attgcaccct gttcaagacg taaaaaagcg ctaaatggcg cgcggttggc ctgattaagc
     9301 tgaaggaatg cctgccattc atcgccagaa taggtcgcat gaaaacgctg ggcgagattc
     9361 acctgatagc aatcaccgct gtgcagatat tcctgtacct ggcgaaattt ttcgccgtac
     9421 tgctcgtggg tcatattgga ttgccagtcg gaagtgagcg tgaaatcttc ctgcggcgag
     9481 aattgctggc tttccagcca ggcccgacgg gcattgacat cattatgact cagcaaagaa
     9541 actgtatgac gctggtggtc gacaatgagc gcccaatcgt agatacccac tgccatatcc
     9601 ggcagaacga tatcttgttc cgcaatttct ggcagtgact caaaacggcg gcccagatcg
     9661 tagccaaaca accccagtgc gccgccctga aatggcaaat cttcgttatg cgttgggcga
     9721 atgtctgcgc gatccagcac ctgctggagc acctgtagcg ggtcatcagt ggtcgttgtg
     9781 cgtttttcgc tttcactaac aacggtttct ttacctaaag tggttaaagt gcaaatcgga
     9841 tcggcgacca caatatcaaa gcggctatac ggatgatcgg catagccgga gtgtaaaagc
     9901 atcgcccacg gcaggtggct taagcgggag aaataaaatt cagcggcgtc ctgacgccag
     9961 ggtaaagtaa tcacagtggg agataacgtc ttcatcagtc ctgactctac tggctatgtg
    10021 ccgggtagca tgaaataatt agcgccaaca atttagcagg agttaacaat gtttgcaggt
    10081 ttaccttcac tcatccatga acagcagcaa aaagctgtcg agcggatcca ggaactgatg
    10141 gcacaaggga tgagtagcgg ccaggcaatc gcgctggtgg cagaagagct gcgtgccaac
    10201 cactccggcg agctaatcgt ggcgcgtttt gaggatgaag acgagtaaca cactaatccg
    10261 tatcgatttc gttatagaag ccgatgatca tttgattgtg agaaaaagga cgctttttat
    10321 atgtgattaa aagcgtcctg acccatttat gctgatataa atttaactca gaccagtgct
    10381 tttagttctt cctgcatata tgttgaaaag tattcaggat ttttaatcac agagcgttca
    10441 cctgtctcca ttttttttgc ccatccaacc attttttccg tgaacccagg gttccagcct
    10501 tcctggtcag ctcgcgccgt aatatcagcg gcactcgcgg gtactcccag ttcttttaaa
    10561 tatttaaaga ttcccttcgc cgtgctttca ttcatcgaac ttggtgttgg cgctgataca
    10621 tccatggcac caatgaaatc cagggcttta tctattacag caggcatatt cttatcctta
    10681 aaattaacca tgttatcgat aaggctatat atgaccttaa tttgcatcta atatcaaagg
    10741 gattataacg atatggcacg agttaacctg ttgacattca ggtgttttaa aggtagcagc
    10801 tcatggtgtg ttatccagta agatggagaa aattaacggt cagcccgcac gctataacca
    10861 gattgttcag ctaagaaaga aaaccagagt tgctaatcaa agggcgaaca caacggcatg
    10921 gttatgttta tttatatagt tagttttata gataaaattg aaattataaa tataaataat
    10981 gaattagcag aagcatcaac agtaatatct tattctctgg tcgatttatg taaaaccgac
    11041 cagtattatc gataaattat cgtctgactt attttgcttc gataaagctt acaccgcagc
    11101 cacaatctta atttcaactt tatacttcgg gttcatcaaa cccgcttgta ccgtgcagcg
    11161 caccggcgca tgacccgcga caacccaggc atcccacgct ttattcatcg ccgcgaagtc
    11221 gtttttatcg gccaggaaaa tggtggcatc cagaatgctc gatttattgc tgccctgttt
    11281 ttccagcacg gcgtcaatct gtgccagcgt gttggcggtt tgctcaaagg catcggcgtc
    11341 gaggttttcc ggtacaccag tgtagtagag cgtgttgttg tggattacta catcagacca
    11401 gcgggcttca gcatcgatac gaacgatagt catcattttg tcctcattat acttccattt
    11461 tcgtgctgca aagactgcca caatgtgagg ttgccgtcac cacttcaact ggcgaaagcg
    11521 ccctcatcct gacataatcc ctgttcaaat caacaggggg tagtgtgacg gacgattttg
    11581 caccagacgg tcagctggcg aaagcgatac caggctttaa gccgcgagaa ccacagcgac
    11641 agatggcggt agccgtcacc caggcgatag aaaaaggcca gccgctggtg gtggaagcag
    11701 gaaccggtac gggcaaaacc tacgcttacc tggctcctgc gctgcgggcg aaaaagaaag
    11761 tcattatctc gaccggctca aaagcgttgc aggatcagct ctacagccgc gatttgccaa
    11821 cagtctcaaa ggcattgaaa tatacgggca acgtggcgct gctgaaaggg cgctcaaact
    11881 acctctgcct cgaacgtctc gaacagcagg cgctggcggg gggcgatctg ccggtacaaa
    11941 tcttaagcga tgtgatcctg ctgcgctcct ggtctaatca aacagtcgat ggtgatatca
    12001 gcacctgcgt cagcgtggcg gaagattcac aggcgtggcc gctggtcacc agcaccaacg
    12061 acaactgtct tggcagcgac tgcccgatgt ataaagattg ctttgtggtc aaagcacgta
    12121 aaaaagcgat ggacgccgat gtggtggtgg taaaccatca tctctttctg gcggatatgg
    12181 tggttaaaga gagtggattt ggcgagttga tccctgaagc ggacgtcatg atcttcgacg
    12241 aagcccacca gctaccggac attgccagcc agtattttgg tcagtcactc tccagtcgac
    12301 aactgctcga cctggcaaaa gacatcacca tcgcctaccg caccgaatta aaagacaccc
    12361 agcagttaca aaagtgcgct gatcgtcttg cccagagtgc gcaggatttt cgtctgcaac
    12421 tcggtgagcc aggttatcgc ggtaacctgc gtgagctgtt agctaatccg caaattcagc
    12481 gggcattttt actgctcgat gacaccttgg aactttgtta tgatgtggcg aaactgtcac
    12541 tggggcgttc cgccttgctg gatgcggcat ttgagcgcgc cacgttgtat cgcacacggc
    12601 tgaagcggct aaaagagatc aatcagccgg gctacagcta ctggtacgaa tgcacttcgc
    12661 gccattttac tctggctctc acgccgctca gcgtggcgga taaattcaaa gagttaatgg
    12721 cgcaaaagcc cggtagctgg atcttcacct cagcaacgct gtcggtgaac gacgatctgc
    12781 atcatttcac ctcgcggctt gggatagaac aggcggagtc gttgctattg ccaagcccgt
    12841 ttgattacag ccgccaggcg ttactctgtg tgccgcgcaa tctgccgcaa accaaccagc
    12901 caggttctgc tcgccagtta gcggcaatgc tgcgaccgat catcgaagct aacaacggtc
    12961 gttgttttat gctttgtacc tcgcacgcca tgatgcgcga tctggccgag cagttccgcg
    13021 ctaccatgac gcttcccgtt ttgttgcagg gggaaaccag taaaggccaa ctgttgcagc
    13081 agtttgtcag tgccggtaat gcgcttcttg tggcaaccag tagtttctgg gaaggggtgg
    13141 acgtgcgtgg cgatacattg tcattggtaa ttatcgacaa attgccgttt acctcgccgg
    13201 atgatccact gttaaaagcg cgcatggaag attgtcgttt gcgcggtggc gacccgttcg
    13261 atgaagtgca actaccagat gccgtcatta ctctcaaaca gggggtaggt cgactgattc
    13321 gcgacgccga cgatcgtggc gtgctggtga tttgtgacta tcggctggtg atgcgtcctt
    13381 acggcgcgac gtttctcgcc agtctgccgc ccgcgccacg cacccgtgac attgcccgtg
    13441 cggttcgttt ccttgcgata ccatcctcca ggtaatttgt tacggagtat ggtatgatgc
    13501 gcgccttttt attgatctaa gcacgaagac ccatgcgaat tctggctatc gataccgcga
    13561 cagaggcctg ctctgtcgcc ctgtggaacg acggtactgt caacgctcat tttgagcttt
    13621 gccctcgtga acatactcaa cgaatcttac cgatggtgca ggatatcctg accaccagcg
    13681 gaacttccct gactgatatt aacgctctgg cttacgggcg cggccccggt agctttaccg
    13741 gcgtgcgcat tggtattggc atcgcgcaag ggctggcgct tggcgcggaa ttaccgatga
    13801 ttggcgtctc cacgctaatg acgatggcgc aaggggcgtg gcgcaaaaac ggcgcaacac
    13861 gcgtgctgtc agccattgat gcgcgaatgg gcgaagttta ctgggccgaa tatcagcgtg
    13921 atgaaaacgg tatctggcac ggtgaagaaa ccgaagccgt actcaaaccc gaactcgttc
    13981 atgaacgaat gcaacagctt tccggcgaat gggtgacggt agggacgggc tggcaagcct
    14041 ggccggatct cggtaaagag agcgggctgg ttttgcgcga tggcgaagtg ttactccccg
    14101 ctgcagaaga tatgctgccg attgcttgtc agatgtttgc cgagggtaaa acggtggcgg
    14161 tggaacatgc cgagccggtt tatttgcgta acaacgtcgc atggaagaaa cttccgggca
    14221 aagaatgaat cttaagagta tgccctgaga aaaaaaggag tcgcatcatg gcggttcaaa
    14281 agaatgttat caaaggcata ctggcaggta cgtttgcgct aatgctgagc ggttgtgtca
    14341 ctgtgccgga cgccattaaa ggcagcagta ccacgccgca acaagattta gttcgggtga
    14401 tgagtgcgcc gcagctgtac gttggtcagg aggcacgctt tggtggcaaa gtggttgcgg
    14461 tacaaaacca gcaagggaaa acccgcctgg aaattgctac cgtaccgctg gacagcggag
    14521 ctaggccgac gctgggagaa ccttctcgcg gtcgcattta tgccgatgtg aacggttttc
    14581 tggacccggt ggatttccgt ggacaactgg ttacggtagt cgggccaatc accggtgcgg
    14641 ttgacggcaa aatcggcaat acgccctata aatttatggt gatgcaagta acgggttaca
    14701 aacgttggca tttaacccag caggtgatta tgccgcctca gccgattgat ccatggtttt
    14761 atggcggtcg tggctggccc tatggctacg gcggatgggg ctggtataat cccggccccg
    14821 cgagagtaca aacagttgta accgaataat tgcttgtttt taaagaatgc agaaacagcg
    14881 gctggtccgc tgtttctgca ttcttacggt aaagataaaa ataaatagtg acgcgcttcg
    14941 caaccttttc gttgggtaat tatcaagctg gtatgatgag ttaatattat gttaacggcg
    15001 tgtatatcat ttggggttgc gatgacgacg aacacgcatt ttagaggtga agaattgaag
    15061 aaggtttggc ttaaccgtta tcccgcggac gttccgacgg agatcaaccc tgaccgttat
    15121 caatctctgg tagatatgtt tgagcagtcg gtcgcgcgct acgccgatca gcctgcgttt
    15181 gtgaatatgg gggaggtgat gaccttccgc aagctggaag aacgcagtcg cgcgtttgcc
    15241 gcttatttgc aacaagggtt ggggctgaag aaaggcgatc gcgttgcgtt gatgatgcct
    15301 aacttattgc aatatccggt ggcgctattt ggcattttgc gtgccgggat aatcgtcgta
    15361 aacgttaacc cgttgtatac cccgcgtgag cttgagcatc agcttaacga tagcggcgca
    15421 tcggcgattg ttatcgtgtc taactttgcc cacactctgg aaaaagtggt tgataaaacc
    15481 gccgttcagc acgtaattct gacccgtatg ggcgatcagc tatctacggc aaaaggcacg
    15541 gtagtcaatt tcgttgttaa atacatcaag cgcttggtgc cgaaatacca tctgccagat
    15601 gccatttcat ttcgtagcgc actgcataac ggctaccgga tgcagtacgt caaacccgaa
    15661 ctggtgccgg aagatttagc cttcctgcaa tacaccggcg gcaccacagg tgtggcgaaa
    15721 ggcgcgatgc tgactcaccg caatatgctg gcgaacctgg aacaggtcaa cgccacctat
    15781 gggccgttgc tgcatccagg taaagaactg gtagtaacgg cactgccgct gtatcacatt
    15841 tttgccctga ccattaactg cctgctgttt atcgaactgg gtgggcagaa cctgcttatc
    15901 actaacccgc gtgatattcc agggttggta aaagagttag cgaaatatcc gtttaccgct
    15961 atcactggcg ttaacacctt gttcaatgcg ttgctgaaca ataaagagtt ccagcagctg
    16021 gatttctcca gtctgcatct ttccgcaggc ggtgggatgc ctgttcagca agtggtggca
    16081 gaacgctggg tgaaactgac cggacagtat ctgctggaag gttatggcct gaccgagtgc
    16141 gcgccgctgg tgagcgttaa cccgtatgat attgattatc atagcggtag catcggtttg
    16201 ccggtgccat cgacggaagc caaactggtg gatgacgacg acaacgaagt accaccgggt
    16261 caaccgggtg agctttgtgt caaaggaccg caggtgatgc tgggttactg gcagcgtccg
    16321 gatgctacag atgagatcat caaaaatggc tggttacaca ccggcgacat cgcggtgatg
    16381 gatgaagaag gattcctgcg cattgtcgat cgtaaaaaag acatgatttt ggtttccggt
    16441 tttaacgttt atcccaacga gattgaagat gtcgtcatgc agcatcctgg cgtacaggaa
    16501 gtcgcggctg ttggcgtacc ttccggctcc agtggtgaag cggtgaaaat cttcgtagtg
    16561 aaaaaagatc catcgcttac cgaagagtca ctggtgactt tttgccgccg tcagctcacg
    16621 ggatacaaag taccgaagct ggtggagttt cgtgatgagt taccgaaatc taacgtcgga
    16681 aaaattttgc gacgagaatt acgtgacgaa gcgcgcggca aagtggacaa taaagcctga
    16741 gcgttaagtc agtcgtcaga cgccggttaa tccggcgttt tttttgacgc ccactaaaga
    16801 gaaaacaatt tgaattacca aatgattacc acggacgatg cgctggcttc tttgtgtgaa
    16861 gccgtccgtg tctttccggc gatagccctg gatactgaat ttgttcgtac gcgcacttat
    16921 tacccgcagc tggggttgat tcaacttttc gatggcgagc atctggcgct aattgatcct
    16981 ctcgggatca ccgactggtc accgctgaaa gcgatcctgc gcgatccgtc catcacaaaa
    17041 tttctccatg caggcagtga agatctggaa gtgttcctca atgtctttgg cgaattacca
    17101 caacccttga ttgacacgca aatccttgct gccttctgcg gacgcccgat gtcatggggt
    17161 ttcgcttcca tggtggaaga gtattccggc gttacgctgg acaagagtga atcgcgcacc
    17221 gactggctgg ccagaccgct gaccgaacgt cagtgtgaat acgcagcggc ggatgtctgg
    17281 tatctgttac cgatcaccgc caagcttatg gtagaaacgg aggcctccgg ctggctacct
    17341 gcggcgctgg atgaatgccg cctgatgcaa atgcgtcgtc aggaagtcgt tgcgccggaa
    17401 gatgcctggc gtgatatcac caatgcctgg cagttacgca cacgccaact ggcctgtctg
    17461 caactgttag ccgactggcg actgcgcaag gcgcgagagc gtgatctggc ggtgaacttt
    17521 gtcgtgcgtg aagagcattt gtggtcggtc gcgcgttata tgccgggaag tttaggcgaa
    17581 ctggacagcc tgggtttatc cggtagcgaa atccgctttc acggtaaaac gctgctggcg
    17641 ctggtggaaa aagcgcagac attgccggaa gatgccttac cgcagccgat gcttaacctg
    17701 atggacatgc ctggttatcg taaagcgttt aaagcgatta agtcgctgat tactgacgtg
    17761 agcgaaacgc ataagatcag cgccgaattg ctggcatcgc gtcggcaaat caaccaactg
    17821 ctgaactggc actggaaact gaaaccgcag aacaatttgc cggagctgat ttccggctgg
    17881 cgtggtgagc tgatggcgga agcattacac aatttattgc aggaatatcc gcagtaaaat
    17941 cttccgaagc cggactgggc gcgctcagcg ccacatccgg ctttggcaaa ctacaaatcc
    18001 aacaccaggc gtttaccctt cgcacgcgaa caacagatca acatactttg ctggctggca
    18061 cgctcttcat cgctaaaata ctgatcccga tggtcagctt caccttccag tattgccgtt
    18121 tcgcaggttc cgcatacccc ttcacgacat aaacattcca ctttcgcggc tttattattt
    18181 tcaataacct gcaaaatagt catctcttcc ggcaccacaa actcttttcc ggaacgggca
    18241 agcaccaggg taaatgcatc gccggttttg tcttcgatag caaattgctc aaagtgcagt
    18301 gtatcggcgg cgatgtccag acgcgcagct tcacttctta ccgcttcaat tagcgcctcg
    18361 gggccacagg tataaacgtg tgtgccaggt tcgatatccg ccaataatcg cgctaattcc
    18421 agtcgtgttc cggttgatga atgatgcaaa tggactttct cagcctgcgg atgctggact
    18481 agctcagcac gaaatgcgca actttctgga tttcgcgagc agtaatgtag ctgccagtcg
    18541 atgtcgctgt gttgcaactc tgccatgtgc gacaggaaag gggtgatacc aataccgccc
    18601 acgataaaca gatgctttct ggctgaggga attagcgcaa acaggttatt aggcgttgag
    18661 atcgttaacc gatcgcccac ttttacctgc tgatgcaaaa agcgggaacc gccgcgcgag
    18721 ttttcttcca gtcgaacggc aatctgataa ctagaggtgt tatgcggtga actcagtagt
    18781 gaatacgcat tgctgtactg gttatcacca tcgctcatct gcactaggat gtgacttcct
    18841 ccggtgaacg caggcagcgg tttgccatcg gttgccacca gcgtgaagcg tttcacctgt
    18901 tcggtaaggg gttcaacctg gctcacctgt acttcaaaca tttgatagtc tgacattgct
    18961 gtactccatc aaaaatcagc ccagcggcgg aaaataccgc cgccgatgtc attagtcctt
    19021 aaacacctgc gccagcagat tatggaaatg ggcgatacca tgttcggaaa tgccactacc
    19081 gctactgtcg gccatgatgc gcccctgacc acgatagcca cgcgatttca gccctttctg
    19141 tacgctttca accagacgta aatcttccgg atggaacaca tcgcgatacc actcaatcag
    19201 cgatttttgc tcgtcggtta actcttcatt ggtgaagtaa atatcgtagt tttgcagggt
    19261 agtttcagaa tccaccggga attcataaat gaccgtcatc atccctttga tcggggtgac
    19321 gttcagcatc gtgcacggcc acagccagaa accgtggaat gccgcatccg taccctcttc
    19381 aaatttaaac gactgttcgg acggtttggc gaaaccgtat tgcagcgtcc agttaccgtg
    19441 catggtgtgc cagtaacgat caacctgtac ggagtcggag aaacctagat gcgccggacc
    19501 acagtgatag cactcgagat agttatcgac aatgttcttc cagttggcag gcgtgcgggt
    19561 ggtaaagcgg gccgctagtt tcagattgtg gacttccggg caggcttcca gcactttcgc
    19621 gcccagaccg gtgtattgat tcacttgaag tacgaaaaaa aaccgggagg acattggatt
    19681 attcgggttc tgatgggatt agatttggtg gggcttgcaa gcctgtagcg caaattttag
    19741 tcgttaatca atgaaacgcg aaagatagta aaaaattgct tttgtttcat tgaaaatacg
    19801 aaaaacaaaa acactgcaaa tcatttcaat aacagcttca aaaaacgttc aaaaccgata
    19861 acaaccaggc tgtaaccaaa tgactcatat cacaaatcag ctcatgccgt ttaggtatgt
    19921 tacatgtgtg attatgtgag gtgaagtatg ttttagctgg ttcatggttg ttatacggct
    19981 ttttttacat cttgtggttc ttgtgaagga gctacaagac cttcctgttc ataaatacca
    20041 tactttgaca aaatcttttt acgagtatct gttggtaatg attcaagcat tttggctgtt
    20101 tgtaaggttt gctgcgaaat accttgcaaa tcagcctgga tggaatcatc ggaagaaaaa
    20161 atctcaggca taagagcttc aaatcctttc tttctgaaca actcaatcgc agaatgcagt
    20221 tccttgtcct ctaacaattc gacaattgcg atcagtttag ttttcaatgt gctgcggtca
    20281 ggttgagaat ttttattc
//
LOCUS       DAJTQQ010000071        20157 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000071 DAJTQQ010000000
VERSION     DAJTQQ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20157
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.071"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            83..352
                     /locus_tag="OO309_002775"
     CDS             83..352
                     /locus_tag="OO309_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCR5847864.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            346..711
                     /locus_tag="OO309_002776"
     CDS             346..711
                     /locus_tag="OO309_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR5847865.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(712..1500)
                     /gene="bioH"
                     /locus_tag="OO309_002777"
     CDS             complement(712..1500)
                     /gene="bioH"
                     /locus_tag="OO309_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR5847866.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1538..2221
                     /gene="gntX"
                     /locus_tag="OO309_002778"
     CDS             1538..2221
                     /gene="gntX"
                     /locus_tag="OO309_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR5847867.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2280..2855
                     /gene="nfuA"
                     /locus_tag="OO309_002779"
     CDS             2280..2855
                     /gene="nfuA"
                     /locus_tag="OO309_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR5847868.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3216..4532
                     /gene="gntT"
                     /locus_tag="OO309_002780"
     CDS             3216..4532
                     /gene="gntT"
                     /locus_tag="OO309_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5847869.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4577..6661)
                     /gene="malQ"
                     /locus_tag="OO309_002781"
     CDS             complement(4577..6661)
                     /gene="malQ"
                     /locus_tag="OO309_002781"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR5847870.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6671..9064)
                     /gene="malP"
                     /locus_tag="OO309_002782"
     CDS             complement(6671..9064)
                     /gene="malP"
                     /locus_tag="OO309_002782"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR5847871.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9676..12381
                     /gene="malT"
                     /locus_tag="OO309_002783"
                     /pseudo
     CDS             9676..12381
                     /gene="malT"
                     /locus_tag="OO309_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12424..13440)
                     /gene="rtcA"
                     /locus_tag="OO309_002784"
     CDS             complement(12424..13440)
                     /gene="rtcA"
                     /locus_tag="OO309_002784"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR5847872.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13444..14670)
                     /gene="rtcB"
                     /locus_tag="OO309_002785"
     CDS             complement(13444..14670)
                     /gene="rtcB"
                     /locus_tag="OO309_002785"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR5847873.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14851..16449
                     /gene="rtcR"
                     /locus_tag="OO309_002786"
     CDS             14851..16449
                     /gene="rtcR"
                     /locus_tag="OO309_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR5847874.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16431..17189)
                     /locus_tag="OO309_002787"
     CDS             complement(16431..17189)
                     /locus_tag="OO309_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR5847875.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17206..18036)
                     /gene="glpG"
                     /locus_tag="OO309_002788"
     CDS             complement(17206..18036)
                     /gene="glpG"
                     /locus_tag="OO309_002788"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR5847876.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18081..18407)
                     /gene="glpE"
                     /locus_tag="OO309_002789"
     CDS             complement(18081..18407)
                     /gene="glpE"
                     /locus_tag="OO309_002789"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR5847877.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18597..20102
                     /gene="glpD"
                     /locus_tag="OO309_002790"
     CDS             18597..20102
                     /gene="glpD"
                     /locus_tag="OO309_002790"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR5847878.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
BASE COUNT         4725 a         5557 c         5114 g         4761 t
ORIGIN      
        1 gagtcagtag atactctacc ctgcttaagt gctactttgt gctatcttgc atgtaacaca
       61 aagtagcact tagggaactg atatgcagac agtgaaatta cggcaacaag gaggagccat
      121 gatcgttact atcccacgag atcttgccat cgatcttggc tggtcaccgg gcacagagct
      181 taccgtggag aaaaaaggcg atagcgttaa tttgcgcgcc acagagcaca aaccgcgtgg
      241 gcgtcttacc gtcgcgcagt tgttgagcca aatcgatgaa agcgaaatta cggagctaaa
      301 tcagtccaca gaaggatggg ctgaagggaa aaaaggtaat gaagcatggt aaaggcacgg
      361 acgccacatc gtggtgagat ctggtatttt aaccctgatc cggttgccgg gcatgaactt
      421 caggggccac attattgcat tgtggtaacg gacaaaaaac tcaacaatgt tttaaaagtt
      481 gctatgtgct gcccgatttc aacaggggca aatgcagcac gttccacagg ggtgacggtg
      541 aacgtcctcc cccgtgatac gcaaaccggt aacctgcatg gcgttgtact ttgtcaccag
      601 ctaaaagccg tcgatcttat tgcccgtggc gctaaatttc ataccgttgc cgatgaaaaa
      661 ttgattagtg aagttatcag taaactggtg aatttaatcg acccacaata attagctctc
      721 acttaccacc aacaccctct gcttcaacgc caccagcagg tgacaaaact cgaccggatg
      781 cgaaataaac ggcgcatggg ccgctttggc gaagatatat gattcgctgt gaggccaaag
      841 tttatccagc atcggcacca ctttgcgcgg caccagaccg tcgagatagc catacaatcg
      901 caaaaacggc atggacacgt tttgcagcgg ctgacggaga tcgaccgttt tcaggatttc
      961 cagcccgcca ttaagcacgt caacctccgg catcggtaac gccagaacgg ttttcttcaa
     1021 cgcccgcgca tcctggcgcg ccgtttcagt ccccatggtt tgtaacgcca ggaaccgctc
     1081 cactgtacgc tgaaaatcat cactgagttg ctgctgaaat cccgccagca cgtccggttt
     1141 tatccccggc cactcgtcac gagcactaaa acaaggtgac gacgccacgg tgaccagcgc
     1201 ctgaacacgc tcgggatggg ttaacgcaat ctggcttgcc accagcccgc ccagactcca
     1261 gcctaaccaa atggctttat caggtgcctg ttgcagcacg gcttcggcca tatcagcaag
     1321 tgacagcgca ccaaatcccc ggctacgccc gaagccgggc aggtcaacaa ggtgcagcgt
     1381 aaaatgcgag ctaagttcct cgtcaatgca acgccacact tcggcattca gtccccatcc
     1441 gtgcagcagc acaagatgaa cattcccctg acctttggtc tgccaccaga tgttattcat
     1501 ccgctattgt tctcttttga cttacaagga tgaacatatg ctaacagtac cgggattatg
     1561 ctggctatgc cgaatgccac tggcgttagg tcattggggg atttgttcgg tctgttcacg
     1621 cgccacccgc acagataaaa cgttatgccc acaatgtgga ttacccgcca cacactccca
     1681 tcttccctgc ggtcgctgcc tgcaaaaacc gccgccctgg caaagactgg tcacggttgc
     1741 cgactatgcg ccgccgttaa gtccgcttat ccaccagctt aaattttccc ggcgcagtga
     1801 aatcgccagc gccctgtcac gtctgttact attggaagtc ttacacgctc gtcgcaccac
     1861 cgggttgcaa ttgccggatc gcatcatcag cgttccgtta tggcagcggc gtcactggcg
     1921 tcggggattt aatcagagcg atttgctgtg tcagccgtta tcacgctggt tgcactgcca
     1981 atgggatagc gaagccgtca cacgtacacg ggccactgcg acccagcatt ttctcagtgc
     2041 ccggctgcgc aagcgcaacc tgaaaaatgc ctttcgtctt gaattgcccg tgcaaggtcg
     2101 ccatatggtg attgtggatg atgtcgttac caccggaagt accgtcgcag agattgcgca
     2161 gttgctttta cgcaatggtg cggcgactgt ccaggtctgg tgcctttgtc gaaccttgta
     2221 gagcctcgat gatgggcgta ttataaccaa ctaaaatagt caactattag gccattacta
     2281 tgatccgtat ttccgatgct gcacaagcgc actttgccaa actgctggca aatcaggaag
     2341 aagggacaca aatccgcgta tttgtgatta accctggcac gcctaacgct gaatgtggtg
     2401 tttcttattg tccgccggat gccgtggaag ccaccgacac tgccctgaaa tttgacctgc
     2461 tgaccgcgta tgttgatgag ttaagcgcac catacctgga agatgcagag atcgactttg
     2521 ttaccgacca gttgggttct cagttaacgc tgaaagcccc gaacgccaaa atgcgtaaag
     2581 tggcagacga tgcaccgctg atggagcgcg tggagtatat gctgcagtcg cagatcaacc
     2641 cacagcttgc tggtcacggt ggtcgcgttt cgctgatgga aatcaccgaa gacggttacg
     2701 ccattctgca atttggcggc ggctgtaacg gttgttccat ggtcgatgtg acgctgaaag
     2761 aagggatcga gaagcagctg ctgaacgaat tcccggagct gaaaggtgtg cgcgatctca
     2821 ccgaacacca gcgcggcgaa cactcctact actaagttat cccctctttt gtagattgcc
     2881 cgatgcgacg ctaaagcgtc atttcgggcc tacgttgaac atgtgccaga cgttggcaca
     2941 gcattgcttt ggcaaatccc tcccttcgtt tccccgcgat aatatgacca acctctcata
     3001 atttaaattt accccgctct ggtgattctc aaacgccaaa tgttacccgt atcattcaca
     3061 tgggtaccaa acatactcct gacatctgac tacaataatt agttttagtg ggtatcagtc
     3121 gtggtgccgc aatatctctg ttcccgattg ggataattag agtttgtcgt cagaaaattg
     3181 acgttaccca taacaaatga aaggccaggt aaatcatgcc attagtcatt gttgctatcg
     3241 gtgtaatctt gttgttgctc ctgatgatcc gcttcaaaat gaacggcttc atcgctctcg
     3301 tcctcgtggc gcttgctgtt ggattaatgc aaggaatgcc gctggataaa gttattggct
     3361 ccatcaaagc cggtgtcggc gggacgctcg gtagccttgc cctgatcatg ggttttggcg
     3421 caatgctggg caaaatgctg gcagactgcg gtggcgcaca acgtatcgcc accacgctga
     3481 ttgccaaatt tggtaaaaag cacatccagt gggcggtggt gttaaccggt tttaccgttg
     3541 gttttgccct gttctatgaa gtgggcttcg tgctgatgct gccgctggtg tttaccatcg
     3601 cggcttccgc gaatattcca ttgctgtatg ttggtgtacc aatggcggct gcactgtctg
     3661 tgacccacgg cttcctgcca ccgcatccgg gcccgactgc gattgccacc attttcaatg
     3721 ccgatatggg taaaaccctg ctgtacggta ctattctggc aatcccgacc gtgattctcg
     3781 ccggtccggt ttacgctcgc gtgttgaaag gtatcgataa gccaatcccg gaaggtctct
     3841 acagcgcgaa aaccttcagc gaagaagaga tgccgagctt tggcgtcagc gtctggacct
     3901 ctctggtgcc ggttgtgctg atggcgatgc gtgcgattgc cgaaatgatc ctgccgaaag
     3961 gtcacgcttt cctgccggta gcggagttcc tcggtgaccc ggtaatggca acgctgattg
     4021 ccgtgctgat tgcgatgttc acctttggtc tgaaccgtgg tcgttcaatg gatcagatta
     4081 acgacacgct ggtttcttcc atcaaaatca ttgcgatgat gctgttgatc atcggtggtg
     4141 gcggtgcgtt caagcaggtg ctggtagaca gcggcgtgga caaatacatt gcttccatga
     4201 tgcacgaaac caacatttct ccgctgctga tggcctggtc gattgctgcc gtactgcgta
     4261 tcgcgctggg ttccgcaacc gttgcggcaa tcactgcggg tggtatcgcg gcaccgctga
     4321 ttgcaacgac gggtgttagc ccagagctga tggttattgc ggtgggttcc ggtagtgtga
     4381 ttttctctca tgtgaacgat ccgggcttct ggctgttcaa agagtacttt aacctgacta
     4441 tcggcgagac catcaaatcc tggtcgatgc tggaaaccat tatctcggtg tgcggtctgg
     4501 taggctgtct gctgctgaat atggtgattt gataacactg actgccggat gcatcgcatc
     4561 cgacatcaac cgcactctac ttcttcttcg ctgcagctct gcgccgtctg tccaaatcct
     4621 tcagcaactt gttcacgcca tcatcggcaa acatcgactc aagcgttgcg gaaagcttgc
     4681 gtcgccagtt tttatactgg taactggtgc caggaatatt caccggttcg gccatatcca
     4741 gccagtcttc cggctgtagt cctaacagag cactgttact gtcggcaatg tagcgctgca
     4801 aaccacggtt cagcgtcggc gtcatcgaca tcaacgatgc cttatgcccg gcacgttttg
     4861 gcagacaacc atatttatgc agtgcatcca gcagcccttg cttcgccagt tcgcgatcct
     4921 gatacagacc gcgcagtacc acttcatccg gatacagccc cagggttttg cccagcgtta
     4981 gatccccgct ctcccagtaa ccacgcagcg ttggcaggtc atgtgtcgcc gcaaccgcca
     5041 tcgactgctc cggatacgct ttcggtgcac ggaacgtctt ctcgtggtcg ttttcgaaat
     5101 agagcacttt gtaagagtac acaccgctgc tgcgcagctt accaacaatc tctaccggta
     5161 cggtaccgag atcttcacca atcaccatac agcgatgacg tttactttcg agtgccagaa
     5221 tcgagagcag atcatccacc ggatagtgaa catacgcgcc ctgatctgcc gtctcgccat
     5281 acggtatcca ccacaaacgc agcatcgaca tcacatggtc aattcgtaat gcgccgcagt
     5341 tttgcatatt ggcacgcaac agctcgataa acggttcata ggcacgcgcg gtgatgatat
     5401 gcgggtccat tggcggtaat ccccagttct gccccaacgg gccgaggata tccggcggcg
     5461 cgccaaccga tgctttcagg caatatagtt cacggtcaca ccaggtttcc gccccacctt
     5521 ccgctacgcc aaccgccaga tcacgataca agccaatcgg catttcatag ccctggctta
     5581 tctcccagca ggcggcaaac tggctgtaag ccagccactg caaccagaga taaaaatcga
     5641 cgtcatcacg atgttcttcg cagaactgac gcacttctgg tgaatccacg ttcttataca
     5701 tctctggcca tgcaggccag ccccagcgca tttcgtcctc tttcacttgc tgggcatgta
     5761 gcgcatcaaa ggcagcctgc cagaacaggc tgtcgccctg ctctgcaaca aactggcgaa
     5821 acgcggccat ctgctcatca tcacgttgcg cgaaaccttt ccacgccatt cgtaatgctg
     5881 tcatttttag ggcggtaacc gtggagtaat cgacccagtc ggcatcgcgc gcctgttgca
     5941 gcgtctgttg cgtggtcggc aactgccacc aggcctgagc ctcttcgcta agatggaaat
     6001 cttcaacggc gttaacgtcg atataaatca cattcagcca acggcgagaa gacgggctgt
     6061 atgggctggc gctctccgga tttgccggat agagcgcatg aatcgggttc aggccaatga
     6121 acgacccgcc acgttttgcc acatccacca gcatcgcttt gagatcgcca aaatccccaa
     6181 taccccagtt tttttccgat cgcagcgtat aaagctgaac gcaggcaccc cacagctttt
     6241 gtttattcag caaagcctgc ggttcgtaac aacgtttcgg ggcgacaatc acccggcaat
     6301 gcgcgcgctg gtcgtcctgg gtgagtgtta gcgtgtgata accttccggc agcttcgtcg
     6361 gtagattgaa cgctttgccc cccgttacat ggcctttgta ctgcgttcct tcttcggtgg
     6421 tcagctgcca gctatattcg ccgctgccct ccaccaccat cggcattttt ttgccgctgg
     6481 tataaaccat gacattcggg actggcgtta ccgccacttt cgtggcggta cgttgatgca
     6541 tcgcgtcaag caaacgccgt ttggtttcgg cgctaatcga ctgcggttta ccgtgggcat
     6601 tgatgtaatt ggggctaatc cccgccgcca gcgcggcatt atccagacgt ttgctttcca
     6661 tcgagcttcc ttagcgtttt gcctgccaga tacgagcctg ataatcgcga atagagcgat
     6721 ccgagctaaa cataccgcag cgggcggtat tgaggatcgc cgcgcgagtc caggcctcct
     6781 ggtcgcggta cagcacatcc acctgctttt gtgcctcaac ataggctgcg aaatccgcca
     6841 tcaccagata cggatcgccg ccctgtttgc cgatactgtg cagcatctgg tcgaaggcat
     6901 gcttatcgcc gtcgctgtat ttaccgcttt ccagctcttt caatactgcg tccagcacct
     6961 tatctttctt ccgccatttc accgggtcgt agcctttggc cagaatagcc ttcacttgtt
     7021 ccacggtatg accaaaaata aagatatttt cttcaccgac tttctcggcg atttcaacgt
     7081 tcgcaccatc cagcgtaccg acagtaagcg caccattgag cgccagtttc atattgccgg
     7141 taccggaagc ttctttacct gcagtcgaaa tttgttcgga gatatccgcc gccgggatca
     7201 gtttttccgc cgccgaaacg caataatccg gcaggaacac caccttcaac ttatcgccaa
     7261 ccagtggatc gttgttgatc acgtcagcca ctttgttgat cgcaaagata atattcttcg
     7321 ccaggtagta gcccggtgcc gctttcgcgc cgaagaggaa gacgcgcggt acgcgatcag
     7381 cctgcaggtt ttcacgaatt tctttgtaca acgccagaat atgcagcaga ttcaggtgct
     7441 ggcgtttgta ctcatgcaga cgtttgatct gaatatcgaa aatcgcctgt ggattgatct
     7501 caataccggt acgaactttc acaaactccg ccagacagac tttattcgcc tgcttgatct
     7561 cgcgatattg ctgacggaat ttcgcatcat cagcgaattt ttccagattg atcagctgat
     7621 cgagatcgtt agcccactct ttttgcagtg atttatccaa cagagccgcc agtgcagggt
     7681 tgcactgttt gatccagcga cgtggggtaa taccgttggt aacgttatgg aatttgttcg
     7741 gccatagctg gtgatattcc gggaacagat ctttcaccac cagatccgag tgcagcgccg
     7801 caacaccgtt caccgcgaaa ccgccaacca cacacaggtt cgccatatgc acttgtttat
     7861 cgtgcaccac cgccagtttg gcccacactt tttcatcgcc cggccaggtt ttctccacca
     7921 gcgttttaaa gcgagtatta atttcgttaa taatctgcat atggcgcggc agtaagcctt
     7981 tcaccagttt cacatcccag cgttccagcg cttctggcat cagggtatgg ttggtgtaag
     8041 cgaaagtttt gctggtgatg gcccaggcgt catcccagct catctggtgc tcatcggtca
     8101 gcacgcgcag cagttctgga atcgcgatag ttggatgggt atcgttcagc tgaataactt
     8161 cgtaatccgc cagttcgtgc agtttacgcc ccgccagatg atggcgacgc aaaatatccg
     8221 ctaccgaaca ggcacactgg aagtattgct gcatcaggcg cagcttttta ccggcagtat
     8281 ggttgtcgtt tggatagaga actttggtca gtttttccgc attgatgcct tgctgttcgg
     8341 cgcgcaggaa atcaccgtcg ttaaatttag ttagatcaaa cggatgcgca tgcgtcgcct
     8401 gccacagacg cagcggctgc gccacgccat tacgatagcc gacaacgggg agatcccacg
     8461 cttgaccggt aatggtaaac tccggctccc agcgtccgtc tttcatcact ttaccgccaa
     8521 tccctacctg cacatccagt gcttcgttgt ggcggaacca cgggtagtta ctgcgatgcc
     8581 agtcatccgg cgcttcaacc tgtttgccat cgacaaaaga ctggcggaac aaaccatatt
     8641 gatagttcag accgtaaccc gtcgcagact gaccgacagt tgccattgag tcgaggaagc
     8701 acgccgccag acgtcccaga ccaccgttac ccagcgccgg gtcgatctct tcttccagca
     8761 ggtcagtcag gttgatgtca taagccttca acgaatcctg tacatcctga taccagccga
     8821 gattcaacag gttgttgccc gtcagacgac caatcaaaaa ctccattgag atgtagttaa
     8881 catgtcgctg attcgccacc ggtttggcga atggctgagc acgcagcatt tcggccagtg
     8941 cttcactcac tgccagccac cactggcgag gagtcatttc agccgcagaa tttaagccat
     9001 aacgctgcca ctgacgtgaa agcacttcct gaaattgctt atcgttaaaa ataggttgtg
     9061 acataggagt tccacttttc ttagattttc aacacaacgt tatcgctagt ttgccaggct
     9121 cgatgttgac cttcctcatc ctgcggggga ttaggcaggg aggagttgcg gggatgagca
     9181 aggaaatgtg atctcgacca cttaaagcta gtgcaaacca caggattagc agcaaatcaa
     9241 tgccatacag cgcagaaaat ctgtatctaa gtgcaaaaaa tggccgttgc gtattttcaa
     9301 aaagcggaag gtaactctat aaattaagta aaggagtgaa acagtttcac aagtaaaata
     9361 tccagtgtgc tccatctcat tcttaataga tttattaaga tcatcttttt agatggcact
     9421 ttcatcagga atgaagaaga aacccttgct taaatgaatc tgatgaacat aagggaaacc
     9481 agtattcacg ctggatcagc gtcgttttag gtgagttgtt aataaagatt tggaattgtg
     9541 acacagtgca aattcagaca cataaaaaaa cgtcatcgct tgcattagaa aggtttctgg
     9601 ccgaccttat aaccattaat tacgaagcgc aaaaaaaata atatttcctc attttccaca
     9661 gtgaagtgat taactatgct gattccgtca aaactaagtc gtccggttcg actcgaccat
     9721 accgtggttc gtgagcgcct gctggctaaa ctttccggcg cgaacaactt ccggctggcg
     9781 ctgatcacga gtcctgcggg ctacggaaag accaccctca tttcccagtg ggcggcaggc
     9841 aaaaacgata tcggctggta ctcgctggat gaaggtgata accagcaaga gcgtttcgcc
     9901 agctatctca ttgccgccgt gcagcaggca accaacggtc actgtgcgat atgtgagacg
     9961 atggagcaaa aacggcaata tgccagcctg acgtcactct tcgcccagct tttcattgag
    10021 ctggcggaat ggcatagccc actttatctg gtcatcgatg actatcatct gatcactaat
    10081 ccagtgatcc acgagtcaat gcgcttcttt attcgccatc aaccagaaaa tctcaccctg
    10141 gtggtgttgt cacgcaacct tccgcaactg ggcattgcca atctgcgtgt tcgtgaccaa
    10201 ctgctggaaa ttggcagtca gcaactggca tttacccatc aggaagcgaa gcagtttttt
    10261 gattgccgtc tgtcatcgcc gattgaagcc gcagaaagca gtcggatttg cgatgacgtt
    10321 tccggttggg cgacggcact atagctaatc gccctctccg cccggcagaa tacccactca
    10381 gcccataagt cggcacgccg cctggcggga atcaatgcca gccatctttc ggattatctg
    10441 gtcgatgagg ttttggataa cgtcgatctc gcaacgcgcc attttctgtt gaaaagcgcc
    10501 attttgcgct caatgaacga tgccctcatc acccgtgtga ccggcgaaga aaacgggcaa
    10561 atgcgtctcg aagagattga gcgtcagggg ctgtttttac agcggatgga tgataccggc
    10621 gagtggttct gctatcaccc gctgtttggc aacttcctgc gccagcgctg ccagtgggaa
    10681 ctggcggcgg agctgccgga aatccaccgt gccgccgcag aaagctggat ggcccaggga
    10741 tttcccagcg aagcgattca tcatgcgctg gcggcaggcg atgcgctgat gctgcgcgat
    10801 attctgctta atcacgcctg gagtttgttc aaccatagcg aactgtcgct gctggaagag
    10861 tcgcttaagg ccctgccgtg ggacagtttg ctggaaaatc cgcagttagt gttattgcag
    10921 gcgtggctaa tgcaaagcca acatcgctat ggcgaagtta acaccctgct agcccgtgct
    10981 gaacatgaaa tcaaggacat cagagaaggc accatgcatg cagaatttaa cgctctgcgc
    11041 gcccaggtgg cgattaacga tggtaatcag gatgaagcgg aacggctggc aaaactggca
    11101 ctggaagagc tgccgccggg ctggttctat agccgcattg tggcaacctc ggtgctgggt
    11161 gaagtgctgc actgcaaagg cgaattgacc cgctcactgg cgctagtgca gcaaaccgaa
    11221 cagatggcac gccagcacga cgtctggcac tacgccttat ggagtttaat tcagcaaagt
    11281 gaaattctgt ttgcccaagg gttcctgcaa accgcgtggg aaacgcagga aaaagcgttc
    11341 cagctgatca acgagcagca tctggaacag ctgccaatgc atgagtttct ggtgcgcatc
    11401 cgagcacaac tgttatgggc ctgggcacgg ctggatgaag ccgaagcgtc ggcgcgtagc
    11461 gggattgaag tcttgtcgtc ttatcagcca cagcaacagc ttcagtgcct ggcaatgttg
    11521 attcaatgct cgctggcccg tggtgattta gataacgccc gtagccagtt gaaccgtctg
    11581 gaaaacctgc tggggaatgg caaatatcac agcgactgga tctctaacgc caacaaagtc
    11641 cgggtgattt actggcaaat gaccggcgat aaagccgccg ctgccaactg gttgcgtcat
    11701 acggctaaac cggagtttgc gaacaaccac ttcctgcaag gtcaatggcg caacattgcc
    11761 cgcgcacaaa tcttgctggg cgagtttgaa cctgcggaaa ttgttctcga agaactcaat
    11821 gaaaatgccc gcagtctgcg gttgatgagc gatctcaacc gtaacctgtt gctgcttaat
    11881 caactgtact ggcaggccgg acgtaaaagt gacgcccagc gcgtgttgct ggacgcatta
    11941 aaactggcga atcgcaccgg atttatcagt cattttgtca tcgaaggcga agcgatggcg
    12001 caacaactgc gccagctgat tcaacttaat acgctgccgg aactggaaca gcatcgcgcg
    12061 cagcgtattc tgcgagaaat caatcaacat catcggcata aattcgccca tttcgatgag
    12121 aatttcgttg aacgtctgct aaatcatccg gaagtgccag aactaatccg caccagcccg
    12181 ttgacccaac gtgaatggca ggtcctgggg ctgatctact ccggttacag caatgaacaa
    12241 attgcgggcg aactggaagt ggcggcaacc accatcaaga cgcatatccg caatctgtac
    12301 caaaaactcg gcgtcgccca ccgtcaggct gccgtgcagc acgcgcagaa actgctgaag
    12361 atgatgggtt atggggtgtg agtttagccg gataatgcat tggatccggt ttacatcttt
    12421 acatcattca atactcaccc gcgttacgcc atctgtttct atcaaaccaa accgcaccgg
    12481 caagaaacgc tccaccaccg cgatattggt cagcagatgg catgaaggat gggcgaccgt
    12541 aaattctccc gcgcccgcca gcgccatcgg cagtaccagt tggtcggcga gatattcccc
    12601 caccgccgct gggcttgcca ggtagcgttt cacctctttc actaactgcg cggctaccac
    12661 ctcggcactg acgcgctttt caccgacgac gaaaaagcgt tcagtgatat tttcactttc
    12721 gacttccagc gagacggtat tacccggccc ctggtcgcgc ggcaggttat gaatattctg
    12781 ttcatgcagg gaaaaaatcc ccgccagtgt agcgatttca cgctcagcaa catggcgcgg
    12841 cacaccagct aatagaactt ctccacgcat ctgcacaatg ttcccgcgct cgccaagttg
    12901 caaggtgcta aacgatgcca cgggcgagac ttccgttgcc accacaccgc ctccggcagg
    12961 ataaaaaccg tgacgtaaca gcgtggtttg ctgatgaatt cctattttcg ccagcagcgg
    13021 ctccagcacc cggcggataa aatccgcagg cggcgccgac gggttatcgg tgccgccgct
    13081 cacttcaaca cgcgaaggtc catcggcaaa ccacagcgcg ggcagcaccg tttgcagcac
    13141 cagcgtacaa cttccggcgc taccgatagc aaagcggtaa tcgccgccgc gcacggtgcc
    13201 aggccggaag accagacgct gcgatcccag ctccgcgcct tccaccgttg ccctacaaat
    13261 ttccgcagcc gcttttaccg cggtcagatg ctggcgcagc agccccggtt tcgcccgtcc
    13321 ggctcgaatg ccagtgatgg taaatggctg gccggttatc atcgacaggc tcagcgccga
    13381 gcgcaggatc tgcccgccgc cttcgccctg tgcgccatcc agcgcaatca tccttttcat
    13441 catttatcct tttacgcaca ccacctgacg cagggtgtag ataacttcca ccagatcgct
    13501 ttgtgccgcc atcaccgcat caatatcttt atacgccatc gggatttcgt cgatcacttc
    13561 ggcatcttta cggcattcca catgcgcggt ggcacgaatt tggtcttcca cgctgaacag
    13621 ttttttcgct ttagttcggc tcattacccg cccggcaccg tggctgcacg aacagaacga
    13681 ctcttcattt cccagcccac ggacgataaa gctttttgct cccatcgaac cgggaataat
    13741 tccatattga ccagcacgcg cagacaccgc acctttacgc gtcacgtaga tctcttcacc
    13801 aaagagctgt tctttttgca catagttgtg gtgacagttg atctcttcca tcgccagcgt
    13861 ttgtggctgt ctgaccgttt tctgcgtaat gctctgcaac gccgttacca cattttccat
    13921 catcgcatcg cggttaaggc tggcgaaaag ctgcgcccag gccacggctc ttaggtaatc
    13981 atcaaagtat tccgtacctt ccataaagta cgccagatca cgcgacggca acgtctcaag
    14041 ttggtcctgc atctcttttt gcgccagatc gataaagtaa gtcccgatgg cgttaccaat
    14101 tccgcgtgaa ccggagtgca gcataatcca cacctgctcc gactcatcaa ggcagatttc
    14161 aataaagtgg ttacccgttc ccagcgttcc caggtgttta tagttgttag tgttaaggaa
    14221 acgcggatat ttttgcgtta accactgata accggcctcc agctcagccc atttcgcatc
    14281 gacgttaaaa ggcgggtttt cccatgcgcc tttatcacgt ttacaacgcc cggtagtacg
    14341 cccgtgtggt acggccgttt caatcgcttg acgcagctcc gccaggtttt caggcaggtc
    14401 tgccgccgtt aacgcggtac gcagcgcgtt cattccacag ccaatatcca cgcccaccgc
    14461 cgccggaata atcgcccctt tggtcgggat cacgctacca atggtggaac ctttacccag
    14521 gtgtacatca ggcattaccg caatatgttt gaaaataaac ggcatcttcg ccgtattaat
    14581 aagttgctga cgcgcatcgg cttctaccgg cacgcctttg gtccacattt ttaccggggc
    14641 attttcagtg gtcagtaatt cgtaattcat tttgtttttc tcttttcgtt gtttgcggtc
    14701 ctgataattg caaccgtcgt gccagaaaat taaataagca gattaattta ttgtttctaa
    14761 aaagattata tctttatatc cgtaaacgga gatttcccgc aaagccgatt taccgataat
    14821 gaaatatcgc cttttataag gatatctaag atgcgtaaaa cagtggcttt tggctttgtc
    14881 ggtaccgtgc tggattatgc cggacgcggc agtcagcgct ggtcaaaatg gcgtccgtca
    14941 ctctgtatat accagcaaga atcgttggtc atcgatcgac tggaattatt gcacgatgcc
    15001 cgctcgcgct cgctatttga aacgcttaaa cgcgatatcg ccagcgtttc gccagaaaca
    15061 gaagtggtgg gcgttgagat tgaactgcat aacccgtggg atttcgaaga ggtttacgcc
    15121 tgcctgcatg atttcgcccg tggttacgag tttcagccag aaaaagaaga ctatttaatt
    15181 cacatcacca ccggcaccca cgtcgcgcag atttgctggt ttctgctggc ggaagcgcgt
    15241 tatctacccg cccggttgat acaatcttca actccgcgca aaaaagaaca gccgcgcggc
    15301 ccaggtgaag tgacgattat cgatctcgat ttaagccgtt ataacgccat cgccagccgc
    15361 tttgccgagg aacggcagca aacgctcgat ttcctcaaat ccggtattgc cacgcgtaac
    15421 ccccacttca accgcatgat tgagcagatc gaaaaagtgg cgattaaatc ccgcgcgccg
    15481 attctgctta acggcccgac cggcgcaggt aagtcatttc tggcgcgacg catcttcgag
    15541 ttaaaacagg cacggcatca gtttagcggc gcgtttgtgg aagtgaactg cgccaccctg
    15601 cgcggcgata ccgcgatgtc gacgctgttt ggtcatgtga aaggcgcgtt taccggggcg
    15661 cgggaatctc gtgaaggatt attacgcagc gccaacggcg gaatgttgtt tcttgatgag
    15721 attggcgaac tgggcgcaga cgaacaggca atgctgctga aagctattga agagaaaacc
    15781 ttttacccgt ttggcagcga tcgccaggtg agcagtgatt ttcagcttat cgccggaacg
    15841 gtgcgcgatt tgcgccagct ggttgccgaa ggcaaattcc gcgaagatct gtacgcgcgg
    15901 atcaatctct ggaccttcac cctgccgggt ctacgccagc gccaggaaga tattgaaccg
    15961 aacctggatt atgaagtgga gcgccacgcc acactcactg gtgacagcgt gcgttttaac
    16021 accgaagcgc ggcgtgcctg gctggccttt gcaacatctc cccaggcaac atggcgcggt
    16081 aactttcgcg aactttctgc cagcgtcacg cggatggcca cctttgccac tagcggacgc
    16141 atcactctgg acgtggtgga agatgagata aaccgtctgc gctataactg gcaggagagc
    16201 cgcccctccg cgcttacggc gttgctgggc gcagaggcag aaaacatcga tctcttcgac
    16261 cgtatgcaac tggaacacgt tatcgctatc tgccgccagg caaagtcgct ttctgctgcc
    16321 ggacgacagc tttttgatgt ttcgcgccag ggcaaagcca gcgtcaatga cgcggatcgg
    16381 ctacgcaaat acctggcgcg ttttggtctg acgtgggaag ccgtgcagga tcagcacagc
    16441 tccagttgaa tatggtggtc cgtcagcacc tgcatcacgc ttactggcgg cggggcgtcg
    16501 gtgtagacgg catcaaccat gctgatgctc cccatattga ccatcgcatt acggccaaat
    16561 tttgaatggt caaccaccag cataacgtgg cgcgagttct caataatggc gcgcttggta
    16621 cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc tgtcgatgcc gctgatcccc
    16681 agaatgccga aatcaaggcg gaactgggag ataaaatcga gcgtcgcttc gccaatgatc
    16741 ccgccatcgc ggctacgtaa ttcgccaccg gcgagaataa tgcggaaatc ttcttttacc
    16801 atcaacgtgt tagcaacgtt gagattgttg gtgacaatgc gcaaattgct gtgattgagc
    16861 agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga taaacagcgt cgagccattg
    16921 gggatttgct ccgccacttt gcgggcgatg cgctcttttt cttcggtctg ggtggcttta
    16981 cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg ccgcgccgcc atgatggcgc
    17041 aggatcaggt tttgctctgc cagctcgttg aggtcgcggc gaatagtctg cgggctgacg
    17101 gagaagtgct ccaccagttc ctcggtactg acataaccct gctgtttaac cagttcgata
    17161 ataccgttgt gacgttgtgt ttgtttcatt tataaatccc tggaattatt ttcgttttcg
    17221 cgcattgagc gaatcaacaa aagccatcgc taaacccacg gctaatccgg cgatgtgtgc
    17281 tccgttcgcc atcgacatcc caaacaaatc aaaccatccg gcgacaatcc agatcagcgc
    17341 aaagataatt aacccacgtt gcaggtaaat gccactttgc ggatcgcgtt cgccacgtag
    17401 ccagacgtag cccatcagcg catacaccac gccagaaagc ccgccaaacc acggcccgct
    17461 gaatttttgc tgcatatagc cgcttaacag ggcgctgata agcgtaatga caattagctt
    17521 accgctaccg aggcgttttt ccaccgcgcc gccgagatac caccaccaaa gcaggttaaa
    17581 gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg aagtaacgcc agaactcaaa
    17641 tttcagtgtt ggatcgaatg gccaggccag ccataacatc acttcctgat cgccgagaat
    17701 ttgcatggca ataaacacca ccacgcaggc gatcatcatc acccaggtca ccggacctgc
    17761 gcgttcacgc aaggcagcaa agaaaggata acggcgataa tgcaggccac tgccggtatg
    17821 gcctgccagc cagctggccg ccagataacg cggatctgcc gggttttcga gaaaacgcgc
    17881 cagctccgcc cgcacgcgct cggcctggga ctcatccgcc agccagacat cgctttggtt
    17941 atgttgttga atcgtgagga taacaccctg cgtcgccatg taatcaacaa acgcctgcgc
    18001 cacgcggggg ttagcaaaag aggtaatcat caacatcgtt gctgtcgctt attccacaca
    18061 aaaggggaca gtataaagcg ttacgcgccg tacgccacct ctgcgggaaa ctgtcgctgc
    18121 caggcttcaa agccgccgtc aatgctatag accacatcgt agccctgttg cagcagatac
    18181 tgcgccgccc ctttgctgct attgccgtga taacacatca ccatcaccgg agtgtcaaag
    18241 tcgttatcac gcataaacgc gcccagcgtg tcgttggtta aatggaaagc ctgcaccgca
    18301 tgccccatag cgaaactctg tggatcgcga atatcgacca gcaccgcctc tttttcctgc
    18361 aacttctggt gcgcgtcggc aacgttaata cattcgaact gatccatgcg tctctctttc
    18421 tttacaaaca agtgggcaaa tttaccgcac agtttacgtc gaagcggcag ataaacgcca
    18481 taatgttata catatcactc taaaatgttt tttcaatgtt acccaaagcg cgattctttg
    18541 ctaatatgtt cgataacgaa catttatgag ctttaacgaa agtgaatgag ggcagcatgg
    18601 aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg tgctggtatc gcggcagacg
    18661 ccgctggacg cggtttatcc gtgctgatgc tggaggcgca ggatctcgct tgcgcgacct
    18721 cttccgccag ttcaaaactc attcacggtg gcctgcgcta ccttgagcac tatgaattcc
    18781 gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct gaaaatggct ccgcatatcg
    18841 ccttcccgat gcgttttcgc ctgccacatc gtccgcatct gcgcccggcg tggatgattc
    18901 gcattggtct gtttatgtac gatcatctgg gtaaacgcac cagcctgccg ggatcaactg
    18961 gtttgcgttt tggcgcaaat tcagtgttaa aaccggaaat taagcgcgga ttcgaatatt
    19021 ctgactgttg ggtagacgac gcccgtctgg tactcgccaa cgcccagatg gtggtgcgta
    19081 aaggcggcga agtgcttact cggactcgcg ccacctctgc tcgccgcgaa aacggcctgt
    19141 ggattgtgga agcggaagat atcgataccg gcaaaaaata tagctggcaa gcgcgcggcc
    19201 tggttaacgc caccggcccg tgggtgaaac agttcttcga cgaggggatg catctgcctt
    19261 cgccttatgg cattcgcctg atcaaaggca gccatattgt ggtgccgcgc gtgcataccc
    19321 aaaagcaggc ctacattctg caaaacgaag ataaacgcat tgtgttcgtg atcccgtgga
    19381 tggacgagtt ttccatcatc ggcaccaccg atgtggaata caaaggtgat ccgaaagcag
    19441 tgaagattga agagagtgaa atcaattacc tgctgaaagt gtataacgcg cactttaaaa
    19501 agcagctaag ccgtgacgat atcgtctgga cctactccgg tgtgcgtccg ctgtgtgatg
    19561 atgagtccga ctcgccgcaa gccattaccc gtgattacac ccttgatatc catgatgaaa
    19621 acggcaaagc accgctgctg tcggtattcg gcggtaagct gaccacctac cgaaaactgg
    19681 cagaacatgc gctggaaaaa ctgacgccgt attatcaggg tattggcccg gcgtggacga
    19741 aagagagcgt gctgccgggt ggtgccattg aaggcgaccg cgacgattac gccgctcgcc
    19801 tgcgccgccg ttacccgttc ctgactgaat cgctggcacg tcactacacg cgcacttacg
    19861 gcagcaacag cgagctgctg ctcggcaatg cgggaacggt aagcgatctc ggggaagatt
    19921 tcggtcatga gttctacgaa gcggaactga aatacctggt ggatcacgaa tgggtccgcc
    19981 gcaccgacga tgccctgtgg cgtcgcacaa aacaaggcat gtggctgaat gcggatcaac
    20041 aatctcgtgt gagtcagtgg ctggtggaat atacgcagca gaagttgtcg ctggcgtcgt
    20101 aaattaacgt aaggtgatca ggtcagattt caatctggcc tgagactgat gacaaac
//
LOCUS       DAJTQQ010000072        19986 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000072 DAJTQQ010000000
VERSION     DAJTQQ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19986
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.072"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            269..328
                     /gene="ibsE"
                     /locus_tag="OO309_002791"
     CDS             269..328
                     /gene="ibsE"
                     /locus_tag="OO309_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCR5847879.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(448..1881)
                     /gene="hldE"
                     /locus_tag="OO309_002792"
     CDS             complement(448..1881)
                     /gene="hldE"
                     /locus_tag="OO309_002792"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR5847880.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1929..4769)
                     /gene="glnE"
                     /locus_tag="OO309_002793"
     CDS             complement(1929..4769)
                     /gene="glnE"
                     /locus_tag="OO309_002793"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR5847881.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4792..6093)
                     /gene="ygiF"
                     /locus_tag="OO309_002794"
     CDS             complement(4792..6093)
                     /gene="ygiF"
                     /locus_tag="OO309_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR5847882.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6335..6955
                     /locus_tag="OO309_002795"
     CDS             6335..6955
                     /locus_tag="OO309_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR5847883.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            7019..8257
                     /gene="cca"
                     /locus_tag="OO309_002796"
     CDS             7019..8257
                     /gene="cca"
                     /locus_tag="OO309_002796"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR5847884.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     IEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8420..9241)
                     /gene="bacA"
                     /locus_tag="OO309_002797"
     CDS             complement(8420..9241)
                     /gene="bacA"
                     /locus_tag="OO309_002797"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5847885.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9332..9700)
                     /gene="folB"
                     /locus_tag="OO309_002798"
     CDS             complement(9332..9700)
                     /gene="folB"
                     /locus_tag="OO309_002798"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR5847886.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9805..10422
                     /gene="plsY"
                     /locus_tag="OO309_002799"
     CDS             9805..10422
                     /gene="plsY"
                     /locus_tag="OO309_002799"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR5847887.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10435..11366)
                     /gene="ttdR"
                     /locus_tag="OO309_002800"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10435..11366)
                     /gene="ttdR"
                     /locus_tag="OO309_002800"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            11573..12416
                     /gene="ttdA"
                     /locus_tag="OO309_002801"
                     /pseudo
     CDS             11573..12416
                     /gene="ttdA"
                     /locus_tag="OO309_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12413..13018
                     /gene="ttdB"
                     /locus_tag="OO309_002802"
     CDS             12413..13018
                     /gene="ttdB"
                     /locus_tag="OO309_002802"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR5847888.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            13066..14529
                     /gene="ttdT"
                     /locus_tag="OO309_002803"
     CDS             13066..14529
                     /gene="ttdT"
                     /locus_tag="OO309_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR5847889.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14572..15585)
                     /gene="tsaD"
                     /locus_tag="OO309_002804"
     CDS             complement(14572..15585)
                     /gene="tsaD"
                     /locus_tag="OO309_002804"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR5847890.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15823..16038
                     /gene="rpsU"
                     /locus_tag="OO309_002805"
     CDS             15823..16038
                     /gene="rpsU"
                     /locus_tag="OO309_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR5847891.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            16149..17894
                     /gene="dnaG"
                     /locus_tag="OO309_002806"
     CDS             16149..17894
                     /gene="dnaG"
                     /locus_tag="OO309_002806"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5847892.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            18089..19930
                     /gene="rpoD"
                     /locus_tag="OO309_002807"
     CDS             18089..19930
                     /gene="rpoD"
                     /locus_tag="OO309_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR5847893.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4763 a         5564 c         5118 g         4541 t
ORIGIN      
        1 tttaggttga aaaaaaatca gcgtcaagaa ggtttcatta acacatatcc ctggcccaaa
       61 aagccgggga ttttttatat ctgcgttccg ctaaaaggtg caaatgctca ggacgttgca
      121 gcgttttgcg tgaccgctcg gggaaggcaa aactgcttca gggaaagcgt tgcgcggggt
      181 ccggcgctca tcaacaatcg gggagtcagc aaggggctga aacgggaaag cccctcccga
      241 ggaaggggcc ttatagaagg aaagggttat gatgaagttc gtcatcatac tggttgtgtt
      301 gttactgtta agtttcccga cttactaaca actgatcaga ggggggagaa atcctccctt
      361 acccttgttc ctttacttta ggttgaaaaa acaacagcgt caataggcct gcctgctacg
      421 aagcgagatc tgtgaaccgc tttccggtta gcctttttta tcctgttgga tcttcttgat
      481 gatgttagtg gtcgagcaac cgtcttcgaa gttgagcacc agcacttcgc cgccgttggc
      541 ccagacttct ttactcccgg cgatctcttc tggtttatag tcgccgccct tcaccagtaa
      601 atctggcagg atcccggcga tcaagcgctg cggcgtgtct tcttcaaacg acaccaccca
      661 gtcgaccgct tccagtgcgc ccagcacaat catacgctgt tcgagcgggt taacggggcg
      721 agaatcccct ttcagccgtt tggtggaggc atcgctgttc acagcgacaa tcaagcggtc
      781 acccagtttg cgggcatttg ccagatagaa gacgtgtccg gcgtgcagga tgtcaaagac
      841 gccattggtc atcaccactt tttcaccgcg tttacgcgct gccgctacgg ccagcttcag
      901 ttcctcttcg gtcatcacgc caaagccggt atctgcgcgc ccacgcactg cattttccag
      961 ctcgatcggc gaaaccgtgg aggttcccag tttgccgacc accacgccag ccgccgcatt
     1021 ggcaaagaag caggcttctt ccagcgaatt acccgctgcc agcgttgccg ccaggacgcc
     1081 aatcaccgtg tcgcccgcac cggtaacgtc atacacttcc tgcgcctggg ttggcatatg
     1141 cagcggcgct ttacccggtt gcagcagcga cataccctgt tcggaacggg tcactaacag
     1201 agccgagagt tcgtaatcgg caatcagttt catgccgcgc tcaacaatct cttcttcggt
     1261 cttacattta ccgacaacag cttcaaattc cgagagattc ggtgtcagca gcgtagcgcc
     1321 gcggtagcgc tcaaaatcgg taccttttgg atcaatcagc accggaacgc ccgctttacg
     1381 cgccagttgg atcatctgct gtacgcttgc cagcgcacct ttggcgtagt cagaaagcac
     1441 cagcgcgcca atcgaactca gcgcctgatt aatccgctcg tgcagcggct gcggatcaac
     1501 accttcgaaa ccttcttcaa aatccagacg gatcagctgt tggttgcggg aaagtacccg
     1561 taatttggtg atggtcggat gcgtcggtac agaaacgaag tcgcatttga cgttgacgtc
     1621 ggccagagat ttactcagcg cgcgcgctgc atcgtcaatg cccgtcaacc cgaccaggcg
     1681 tgcattagca ccgagagaag cgatattcat cgccacgtta gccgcgccgc ccggacgttc
     1741 ttcgatggta ttcactttaa ccacgggcac cggcgcttcc ggcgagatac gactggtggg
     1801 gccgtaccag taacgatcca gcatcacatc accaaccacc atcactcctg cacgttcaaa
     1861 ctctggcagc gttactttca ttcctgtctc ctgagagatt caaaatttgc gcgcgataat
     1921 accatacttc attcttccac cagccacttc tgccagcttg cccgcaccag atcacgctct
     1981 gcggtgaagc aatcctccga cacatggccc ggcaattcct gtaatgccag atgatgaagt
     2041 tcatcgcgca gcgtcgtgta agcacgggtc agcgccatcg cttcctgctc ttccataatg
     2101 tcgttttgcg ccagcagctc cagaatacgc acgttgtctg accagcgcgt taacttcggt
     2161 ttttcatgag cgtagcgcaa caccagatat tgggtaataa attcgatatc ggtaattccg
     2221 ccttcatcgg ctttgatatc aaagcgatta cgatgtttat tgccgagatg agcgcgcatt
     2281 ttctcgcgca tttcccgcac ttccgtttgc agagttttac cttctcgcgg cagtgtcata
     2341 atctcacgac gcaccgcgtc aaagtgcgca gtgagctgcg gatcgccgta cactacacgc
     2401 gcacgcacca gcgcctgatg ttcccacgtc caggcctcgt ttttctgata atcggcaaat
     2461 gcttctgcgg atgtcaccag cattcccgcc gccccggacg gacgcagtcg agcatccact
     2521 tcatacaaaa tgccggaaga ggtacgcgta ctgaacagat gcataatgcg ttgcgccaga
     2581 cgcagataaa actgccgccc gtcgatttcc cgctcgccgt cagtcatcgc atccattggg
     2641 caatcatgga ggaagataag gtcaagatcg gaactgtagc ctaactccca gccgcccagc
     2701 ttgccgtagc cgaccaccgc aaaaccgcgc ccttcacgtt cgttgaggtg atttggctta
     2761 ccgtagcggg ccaccatttg accccacgcc tgctgaacga cggcatctat catagcttct
     2821 gccagccagg ttaagtgatc gctcactttc attaccggta gcgtaccggc gatatccgct
     2881 gccgcaatgc gtaacagctg cgcctgtttg aactgacgca gcgcctcaag ctgttgttct
     2941 tcgtcatctt ccggcacgcg cagcaaatac tggcgcagtt catcacggta ggcatcggtc
     3001 gccgtcggct gataaagggt atttggatcg agcaattcat ccagcaataa tggataacgc
     3061 gctagctggc tggcaatcat cggcgatgcg gcacacagag aaatcaaatg tttaagcgcc
     3121 gcagggaatt cactgagcaa ttctaaatag gttgtgcggg taacaatccc caccagcaag
     3181 gcggtaatgc gcgacagcgt gacagcagcg tcttcacgcg cacagacatc acttagcaga
     3241 tgtggcatca gatggtcgag cacctgacgt cctcgcggcc cgatagtacg cttatccagc
     3301 tctttgcgga aatcagcaat catcgttagg acctgtttgc gatcatcctc gctaagatgc
     3361 gccagcactg gcgtagtgtc atcttcctgc aacgcatcct gccacagctc acgccactgt
     3421 tccgacagcg actcttcctg agtttcactt tcgtcgtcgc caatcaattc attaaacacc
     3481 cggcgcacat tggtcatatg tgcggtcagc gccccggtca gttgcggcca gtcagcaaag
     3541 tccatcgccc acgccagccg cgcacggtta agctcatcag aaggaagcgt ttgggtttgt
     3601 tcgtcgttaa tgctttgcag caggttttcc agacgccgca ggaacagata tgccactcgc
     3661 aattgttcag catcgttttc agaaagcaga tgcagcgcgg caatggcgct gagcgttggc
     3721 agtaaagagc gcgactgcag caacggttcg cgcccaccgc gaatgagctg gaacacctga
     3781 acgataaatt caatttcgcg aatgccgcct gcgccgagtt tgatattgtc ggtcaaacca
     3841 cgtcgacgca cttcacgggc aatcatccct ttcatgttgc gcagcgactg aatcacgctg
     3901 aaatcgatgt aacgacggaa aacaaacggg cgcagcatcg cacgcaactc gttagcatag
     3961 acgccttcgc tatcgcccat aatccgcgct ttgaccatcg cgtaacgctc ccagtcgcgt
     4021 ccctgctcct ggtaataatc ctccaacgcg gcaaagctca gcaccagcgg gccactttcg
     4081 ccaaacggac gcagtcgcat atccacgcga tagacgaagc catcctgcgt tggttgatcc
     4141 agcactttaa tcagccgctg ccccatgcgg gtaaaaaact gcgcgttatc cagttcccgg
     4201 cgtccgccct gcgtacaacc atgttccggc caggcaaaaa tcagatcgat atcagaggag
     4261 aaattcagtt ccccgccgcc cagcttaccc atgcctaaga tcagcagcgg ttgcgcttcg
     4321 ccctgcgcat tgcacggcgt tccccactcg cggcagcagg cgtcatacag ccagtcacgc
     4381 gccgcaacaa tcagcgtctc cgccagataa ctgagctgct gcaatatgct ctcttcagta
     4441 accagtgcca gcgtttgcgc ccatgcgatg cgcaccataa tgcgccgccg gaatagccgc
     4501 agctcgcgca ttaacccggc ttcgtcgctc acattactga gcgcctcctg caaccacacg
     4561 ctgtaatgtt gccattcgtc agcctgtggc ggttggcttt ccagttccgt cagccactct
     4621 ggatgcgcaa tcacgctgtc ctgcacaaaa tcactgaaag taagtactga cttcgcctgt
     4681 gcactaagtg attcctcggc taaaagctct ggcagccgct caacaacggt ctgccagtac
     4741 tgctgtaacg gtgaagagag cggcttcata agcgatttta tccttgcctg attaacgttt
     4801 tccgctgtgc aaccagaacg gttcctgatt gtttgcctca ttacggaaat gttcaatttc
     4861 aatgcgttgc ccggtcgcaa tagcgtgacg cagcccctgc cagttctcca gccaggcttg
     4921 cgcgacgaca ggatcatagt aacccgccag caacagtatt gagtcaatat cacgcatcag
     4981 gcgtggcaac tggtcacggt agcgatcgcc taacggctgg cagaaaacgc ttttcagttc
     5041 agcggcatgg cgggaaagat ggatatcggc aaagcgtttg aaggagtcgc taattttgcc
     5101 ctgcgctttg gcatctaaaa acggctgcca tgctttgctt accagccatt cggtcaacgc
     5161 cagcttcgcc attgccgttt cggtagagta gaccgccgtc acggcagaaa ccgcagaagc
     5221 gatggtcgct tcgcattgag tcagcaaatc acgtaagtga gtactcgctt tacgcggcac
     5281 aatgccaccg aacagcatca gtgtatgacg gactaggcta atggctgcca gcacctgctc
     5341 tttcgccgca tcgttgccgc gtacccacag ttcttcatga tactgccatt gcgctaacac
     5401 cagctcgagc gccgcttcca gcccctgttc cacatcggct tttgccgcaa catgcaaaat
     5461 ggtggtcggt ttgatttcac gcgccggatt gccctgcgcc agatgatagc cacgcgccgc
     5521 tttgctcagg ctgccctggc gtaatccggt ttgcgatacc agttggttcg ccagtttcag
     5581 caccgcgcgc gtgtcgccgc taagcagctc cagttccagc tcgcagatag gctcagcaaa
     5641 ttcacccgct ttcacttccc cctggtcgag agcgatttca attcggctgc catcgatctc
     5701 tactagccat ttttcgcgat aaaaatcggt gctaaacagc gactgcacgc gggaagcgag
     5761 atcggcaggc aattcgccgt tcggccagat ttccgtcggt aactgcgcca ggtcgagcgt
     5821 cggttcgctc aacgccacgt tatattccgg gcgctggtgt aagccgcctg tcactcttcc
     5881 tgcaactttc atggtcatct catagcgacc gttttcgcca cgaatacgta agcccatatc
     5941 gtgcctacgc agccagttat ccggcgtttc gtagtaaata ttcagcaact gcacggggtc
     6001 atggtgctcg ccgcccagcg tattgagatg gtcacgcaac gcctcaacgg cactgtgatt
     6061 aacaataaac tttaattcga tttcctgagc catggccttt acttatcggt tatgtcacat
     6121 aagtgacgat gaacggcgaa cttaatgcga tcttttttgt cagtagatag tattttgcgc
     6181 caaattgcca tgcaacgggc aatttgacgg gcgtaaaagt ttgaagcagt ggcaagacct
     6241 gacacagagg attccgattg atgtcgaatg ctttgcgtag cgacactgat gccactacta
     6301 tcgttccact ttccatgaca ataacgacag cctgatgcca aaattacgcc tgatcggatt
     6361 aactttactc gcacttagcg cgactgccgt ctcacacgct gaagaaacgc gctatgtttc
     6421 tgacgaactg aatacctggg tccgtagcgg tccgggagat cattatcgcc tcgtgggcac
     6481 ggttaacgcc ggcgaggaag tgaccttatt acaaactgac gccaacacca attatgccca
     6541 ggtgaaagac agctctggcc gtaccgcctg gatcccgttg aaacaactta gcactgagcc
     6601 aagcctgcgc tcccgtgtgc cagatctgga aaatcaggtc aaaaccctga ccgataaact
     6661 caccaatatc gataacacct ggaatcagcg caccgcagaa atgcagcaaa aagtggcgca
     6721 gagcgacagc gtgatcaacg ggttaaaaga agaaaatcag aaactgaaaa acgagctgat
     6781 tgtcgcgcag aaaaaggtcg atgccgccag cgtacagctg gatgacaaac agcgcaccat
     6841 catcatgcaa tggtttatgt atggcggtgg cgtgctgggg cttggcttgc tgctcggtct
     6901 ggtactgccg cacctgatcc caagccgcaa acgcaaagat cgctggatga actaaatcgc
     6961 cttcttcgtt gcactgtctt attattagga attgtgtatt tgacgaaggg atgatggcgt
     7021 gaagatttat ctggtcggtg gtgctgttcg ggatgcattg ttaggactat cggtcaaaga
     7081 cagagattgg gtggtggtcg gcagtacgcc acaggagatg ctcgacgcgg gctaccagca
     7141 ggtaggccgc gattttcctg tgtttctgca tccgcaaacg catgaagagt atgcgctggc
     7201 acgtaccgaa cggaaatccg gttccggtta caccggtttt acttgctata ccgcaccgga
     7261 tgtcacgctg gaagatgatc ttaagcgtcg cgatctgacc attaatgcgc tggcccagga
     7321 cgataacggc gagattatcg acccgtacaa cggtctgggc gatctgcaaa atcgtctgtt
     7381 gcgccatgtt tcccccgctt ttggcgaaga tccgttacgc gtactgcgcg tggcgcgttt
     7441 tgctgcgcgt tatgcccacc tcggttttcg tattgccgat gaaactctga cgctgatgcg
     7501 cgagatgacc catgcgggtg aactggaaca cctgacgcct gaacgggtat ggaaagagac
     7561 ggaaagcgcc ctcaccaccc gcaatcccca ggtgttcttc caggtactgc gcgattgcgg
     7621 cgcacttcgc gttttattcc cggaaattga cgcactgttt ggcgttccgg cacctgccag
     7681 gtggcatccg gaaatcgaca cgggtattca taccttaatg acgctctcaa tggcggcgat
     7741 gctgagtccg caggttgatg tccgtttcgc aactttatgc cacgatctcg gtaaaggact
     7801 gacgccgcca gaactttggc cgcgtcatca tgggcatggc ccggcgggtg ttaagttagt
     7861 ggaacaacta tgccagcgtc tgcgcgtgcc aaatgaaatt cgcgatttag ccagactggt
     7921 ggctgagttt cacgatctca tccgcacctt cccaatgctg aacccaaaaa ccatcgtcaa
     7981 attatttgat tccatcgatg cctggcgtaa accgcagcgt atcgagcaac tggcgctgac
     8041 cagcgaggct gacgtgcgcg gcagaaccgg ttttgaatca gcggactacc cgcaaggccg
     8101 ttggttgcgc gaaggctggg aagtggcgca gtcagtgccg acaaaagcgg tcgttgaagc
     8161 gggatttaaa ggcgtggaga ttcgcgagga gctgacccga cggcggattg cggcggtagc
     8221 cagctggaag gaacaacgtt gcccaaagcc tgactgagta taaaactaaa cgttagcacg
     8281 aaccgtcccc tcgccccttc ggggagaggg ttagggtgag gggaacagac cagcaccggt
     8341 gcgaacaatc acccccaccc cggccctctc cctacagagc gagggagaaa acccggcctg
     8401 ctcccgaacc ttttctcaat caaaagaaca cgacatacac cgcagccgcc acaataaagc
     8461 gataaatggc gaacgggata aacgaaatgc gcttaatcaa ttgcaggaag gttttaatcg
     8521 ctatcagcgc caccacaaaa gcggtgataa acccaacggc aaacatcggg atatcgccgg
     8581 ttgtcaggaa gccccagctt ttgtagagat cgagcgccgt tgcgcccatc atcatcggta
     8641 ccgccagcag gaacgagaac tcggaagcag cgtaacggct cacgcccatc agcatcccac
     8701 ctgaaatggt cgccccggaa cgggagaaac ccggccacag cgccagacac tggaaacagc
     8761 caatcataaa cgcctgacga taggtcatat catcaagacc cggcgcacgc ggctctttcg
     8821 gcttcaggca ttcggcggca atcagcaaca aaccgccaac gaccagcgca tacatcacat
     8881 ttatcgggtt aaacaatgac ttaatcgtgt cgtggaataa tagccccagt accaccgccg
     8941 gaatcatccc cagcaaaatg tggatcagcg ttaaacgacc tttgctttca ccttcgtgct
     9001 gcaacgggcg gccaaagtgg atgccaatca ggccaaacag acgccgccag aacatcacta
     9061 ctaccgccag aattgatcct aactggatca caacttcaaa ggttttcgcc gtgtcgccct
     9121 caaaccccaa caagtgaccg acaataatca tatggcccgt gctggatacc ggcagaaatt
     9181 ctgtcaatcc ttcgaccaca cccaatattg ccgctatcag cagcgagtgc atatcgctca
     9241 tcaataaacc cctaaattat taaaatgtac cgcttgtccg aactactgca gtatgaccag
     9301 gttataaccg tttggtttaa cagctgtaaa attaattatt ttctttcaga ttattgccac
     9361 gctcaatgat tacgccaaca ttcgccgccc gcgccactgc gcctggcttg ctgagtttga
     9421 tacgcaccca cggcgagttg aagcgtgcta acagcagctc cgccacctct tcagccacgc
     9481 gttccaccag cgcaaaacgc gccccctcga cgtggctgac caccgtttct gcaatgtcag
     9541 cgtaactgag gcaatccgcc acatcatcac ttttcgccgc tttacggtta tcccatgcca
     9601 tttcgatatc gaacactaac ttctgttcga tggtctgttc ccagtcgtaa acaccaatag
     9661 tggtgattac cgaaagttgc tctataaata caatatccat cacgtcctgc ctgctttttg
     9721 gctaaccgga taccacttcc ggcgaaatgt gcgtattatc cacagattca tcgttgaaca
     9781 cgaattttca aaacggaaca gcttatgagt gcaatcgcgc ctggaatgat cctcatcgcg
     9841 tacctctgcg gctccatttc cagtgccatt ctggtttgcc gcttgtgtgg gctgcccgat
     9901 ccgcgaacca gcggctccgg caatccaggc gcaaccaatg tgttacgtat cggtggcaag
     9961 ggagcagccg tagcagtact gattttcgac gttctgaaag gaatgttgcc tgtctggggc
    10021 gcgtatgaat taggtgtcag ccccttctgg ctaggcttaa ttgccatcgc cgcctgtctt
    10081 ggacacatct ggcccgtttt cttcggtttt aaaggaggaa aaggcgttgc taccgctttt
    10141 ggagcaatcg caccaattag ctgggatctc accggagtaa tggcgggaac ctggttactg
    10201 accgtgctat tgagcggata ctcgtcgctg ggagcgattg tcagtgcact gattgctccg
    10261 ttttatgtct ggtggtttaa gccacaattc accttcccgg tttcgatgct ctcttgcctg
    10321 atcctgctgc gtcatcatga caacatccag cgtctgtggc gtcgtcagga gacaaaaatc
    10381 tggacgaaat tcaaaagaaa gcgcgaaaag gatcccgagt gatttctggt ggatctacat
    10441 gacctgatag ccttcatcgg gcttgcccag ccgttgctgg caccatgccg ccagaaattc
    10501 cgcgcagaca cgaatttcat gctgcgatag agcagctccc ggtaaacagc ccagatattg
    10561 gcgctttgtg catactccgg caatacttgc accagtttgc cactctccag aaacggcaac
    10621 acatcccact cggaacgcag cataatccct ttgccctcca gcgcccattg caacactatc
    10681 tcgccgctat tggaggaaag atgcccgcta accttcaccg attttttctc ctgaccgttc
    10741 cccaactccc atattccgtg ggtcatatcg cgctctttgg tcaccagaca gtcatgacga
    10801 cttaattcct gtaatgattg tggctgcgga tatttttgca gatactcagg tgctgcgcat
    10861 aatattcttt tatttttcgt taacagatgc gcaatataat aatcaggaat ttcgtcatta
    10921 atacgaatat ccagatcaat attatcctgc actaaatcaa tttgccgatc gaacaattca
    10981 aaatgcacct gtaactcagg ataattgcgc ataagttcgg taatggctgg cgcaatatgg
    11041 ctgcgcccaa aaccaaaact gcaaccaata cgaatcatcc cctccgggcg cgttttgatt
    11101 tgcgtgacgt catcgaccag tcgctgatat tgggtgagga tttccagcgc atgttcgtaa
    11161 caacgttggc cactttcagt cagcgccacg ccgcgagcgg agcggttcag cagcgttgtt
    11221 gccagcgtat tttcaaggat ttggatccgc ttggtgacaa atgcaggcgt ttgcccaagc
    11281 gtcgccgctg cagcactaaa actcccgctg tggacgattt ctaccagtac ctgcaagtct
    11341 ttggctaaag gccagctatt cagcatcgtt aattatccgc agttgtgata agcgcagtgt
    11401 atttcgcaaa acattgccat tccctgctct ggcgcaatat tcattcttat tttcgcgttg
    11461 attcacgaac tgttaattac atattaacgg tggcaaaaaa tataaaacca catttttagt
    11521 gttagtttgt ggcggtgaat ttttccagac aaatacaaaa actggagttg ccatgatgag
    11581 cgaaagtaat aagcaacagg cagtgaataa gttgacagag attgtcgcta actttaccgc
    11641 catgatttct acccgaatgc ctgatgacgt ggtggataaa ctaaaacagc taaaggatgc
    11701 cgaaacgtcg tcgatgggga aaattatcta ccatacgatg ttcgacaaca tgcaaaaagc
    11761 gattgacctg aatcgtcctg cctgtcagga caccggggag attatgttct tcgttaaagt
    11821 cggttcccgc ttcccactgc ttggcgagct gcaaagcata ctcaaacaag ccgtggaaga
    11881 ggcaaccgtc aaagcgccac tacgtcacaa tgcggtagaa atttttgacg aagtaaacac
    11941 cggcaaaaat accggtagcg gcgtaccgtg ggtcacctgg gacatcatcc ccgacaatga
    12001 cgatgcggaa atcgaagttt acatggcagg cggcggctgc acgctacctg gccgctcgaa
    12061 agtgttaatg ccgtcagaag gctacgaagg cgtggtgaaa ttcgtcttcg aaaatatctc
    12121 caccctcgcc gtaaacgcct gtccaccggt actggtgggc gtgggcatcg ccacctcggt
    12181 ggaaaccgcc gccgtactct cgcgtaaagc cattttgcgc ccgattggca gccgccatcc
    12241 caatccaaaa gcggcagaac tggagctacg cctggaagaa ggactcaacc gtctggggat
    12301 tggtccacaa gggctgaccg gcaacagttc agtgatgggc gcatcgtcgc ggcacgctgc
    12361 tggttcatgc cgatctcacc tttgaaaatc tgtctcacac ccggagcgcg ttatgaaaaa
    12421 gatcctgaca accccgatca aagctgaaga tctgcaagat attcgcgtcg gcgatgtgat
    12481 ctacctgacc ggtacgctgg tgacctgccg cgacgtttgt caccgccgtt tgatcgaact
    12541 gaaacgtccg atcccttacg atctcaacgg caaagcgatt ttccacgctg gccccatcgt
    12601 gcgcaaaaac ggcgacaaat gggagatggt ctccgtcggc ccgacaacca gtatgcgtat
    12661 ggaaagtttt gaacgtgaat ttattgagca gaccggcgta aaactggtgg ttggcaaagg
    12721 tggtatgggg ccgctgaccg aagaaggctg ccagaaattc aaggcgttac atgtgatttt
    12781 cccagcaggc tgcgcggtgc tggcggcaac ccaggtagaa gagattgaag aagtgcactg
    12841 gacagaactc gggatgccgg agtcactgtg ggtctgccgg gtcaaagagt tcggcccgct
    12901 gattgtctct attgataccc acggcaacaa cctgatagcc gaaaacaaaa agctgttcgc
    12961 cgaacgccgc gatcccatcg tggaagagat ctgcgagcac gtccattaca tcaaataacc
    13021 ctcccggaga ggctcacccc tctccttttt cgcaggcata acacgatgaa accttccact
    13081 gaatggtggc gatatcttgc gccgctggcg gtcatcgcca ttattgctct acttccggtt
    13141 cccgcagggc tggagaacca tacctggctc tactttgccg tttttactgg cgtgatcgtt
    13201 ggactgatcc tcgaacccgt gccgggtgcc gtggtggcga tggtgggtat ctccatcatc
    13261 gccatactct ctccctggct gctgttcagc cccgaacagc tcgcccagcc aggctttaag
    13321 ttcaccgcca aatccctctc ctgggcagtt tctggttttt ctaattcggt tatctggctc
    13381 attttcgccg cctttatgtt tggcacaggc tatgaaaaaa ccgggcttgg acgccgcatt
    13441 gcgctgattc tggtgaaaaa gatggggcat cgcacgctgt ttctcggcta tgcggtgatg
    13501 ttctccgagc tgatcctggc acctgtaaca ccgtccaact cggcgcgtgg tgcgggtatt
    13561 atctatccca tcatccgtaa cctgccaccg ctctatcaat cacaaccaaa cgacagcagt
    13621 tcgcgcagca ttggctcgta catcatgtgg atggggattg ttgccgactg cgtgaccagc
    13681 gccattttcc tgacggcgat ggcacctaac ttgctgttaa ttggactgat gaaaagcgca
    13741 tctcacgcca cgctgagttg gggcgactgg ttcctcggga tgttgccgct cagcatttta
    13801 ctggttctgc tggttccctg gctggcttac gtgctgtacc cgccggtact gaagtctggt
    13861 gatcaggtgc cgcgctgggc agagacggaa ctgcaggcaa tgggcccgct ctgttcgcgt
    13921 gaaaaacgga tgctggggct gatggtaggc gcgctggtgc tgtggatttt cggcggtgat
    13981 tatatcgatg ccgcgatggt cggttacagc gtggtggcgc tgatgctgct tctgcgcatt
    14041 atcagttggg acgacattgt cagtaataaa gcggcatgga acgttttctt ctggctggcc
    14101 tcgcttatca ccctcgcgac cggactcaac aataccggtt ttattagctg gtttggcaaa
    14161 ctgttagcag gcagcttaag tggttattcg ccgacgatgg tgatggtgac gttgattgtg
    14221 gtgttttatc tactgcgcta ctttttcgcc agcgccacgg cgtatacctg cgccctcgca
    14281 ccgatgatga tcgccgccgc gctggcgatg ccggaaatcc cgctgccggt gttctgcctg
    14341 atggttggcg cggcaattgg tctggggagc attcttacac catacgccac cggacccagt
    14401 ccgatttact acggtagtgg ttatctgcca acggcggatt actggcgact gggggcgatt
    14461 tttgggctga tattcctcgt gttgctggtg attaccggct tactgtggat gcccgtggtg
    14521 ttgctttaag tgaggacggt gccggaaatt gctctccggc acccaatagt tttacgcagc
    14581 cggtaactcc gccagcggcc agcgcggacg cacgctaacg ccgagatccg ccgtcgcgcc
    14641 tgctttaaac cgcaccattc cggcataggc gatcatcgcg ccgttatcag tacaaaattc
    14701 cggacgcgcg tagaacactt cgccgcggcg ttttttcatc atttcagcca gcttcgcccg
    14761 taacgtgcgg ttagcactca cgccgcccgc catgaccagt cgcttaaagc ccgtctgatc
    14821 caacgctcgc ttacacttaa tcatcaacgt atcgaccacc gcatcttcaa aggcgcgggc
    14881 gatatcagca cgcgtctggt cgtcggtgcc gttgtcacga atcgtgttcg ccgcaaaggt
    14941 tttcagacca gaaaagctga aatccagccc cggacggtcg gtcatcggac gcgggaaaac
    15001 aaagcgcccg gcagtaccct gagccgccat tttcgacagt aacggtccgc caggataatc
    15061 cagccccagc agcttcgcgg ttttatcaaa cgcttcaccg gcggcatcat cgatagactc
    15121 gccgagcagc tcgtactgac caatgccagt cacgctgatt aactgcgtat gaccgccgga
    15181 caccagcagc gcgacaaacg gaaattccgg cgggttatct tccagcatcg gcgctaacag
    15241 atgcccttcc atatggtgta ccgggattgc cggaacgttc caggcaaacg ccagagaacg
    15301 ccccacggtt gcgccaacca gcagtgcgcc gactaatcca gggcctgcgg tataggccac
    15361 agcatcaatg tcttttgccg ttaaaccaga ctcctttagc gccgcctgga tcaacggtac
    15421 ggttttacgc acatgatcgc gggaggccag ttcaggcacg acgccgccgt agtcagcgtg
    15481 caatttcacc tgactataca attggttggc taacaaacct ttttcatcgt cgtaaatggc
    15541 gatgccggtt tcatcgcagg aagtttcaat acccagtaca cgcatgactt gttttacctc
    15601 gctttattac cgcgcagtgt aggaccaatg cgggttgatg taaaactttg ttcgcccctg
    15661 gagaaagcct cgtgtatact cctcaccctt ataaaagtcc ctttcaaaaa aggccgcggt
    15721 gctttacaaa gcagcagcaa ttgcagtaaa attccgcacc attttgaaat aagctggcgt
    15781 tgatgccagc ggcaaaccga attaatcaaa ggtgagaggc acatgccggt aattaaagta
    15841 cgtgaaaacg agccgttcga cgtagctctg cgtcgcttca agcgttcctg cgaaaaagca
    15901 ggtgttctgg cggaagttcg tcgtcgtgag ttctatgaaa aaccgactac cgaacgtaag
    15961 cgcgctaaag cttctgcagt gaaacgtcac gcgaagaaac tggctcgcga aaacgcacgc
    16021 cgcactcgtc tgtactaatt ccccgagagc gttgctctcc gatcagaccg agttgtagtt
    16081 gtaaggccgt gcttccgaaa ggaatgcgcg gcttattttc gtttatgaat tgctaaaaat
    16141 cggggcctat ggctggacga atcccacgcg tattcattaa tgatctgctg gcacgcactg
    16201 acatcgtcga tctgatcgat gcccgtgtga agctgaaaaa gcagggcaag aatttccacg
    16261 cgtgttgtcc attccacaac gagaaaaccc cgtcattcac cgttaacggt gagaaacagt
    16321 tttaccactg ctttggatgt ggcgcgcacg gcaacgcgat cgacttcctg atgaactacg
    16381 acaagctcga gttcgtcgaa acggtcgaag agctggcagc aatgcacaat cttgaagtgc
    16441 catttgaagc aggcagcggc cccagccaga tcgagcgcca tcagaggcaa acgctttatc
    16501 agttgatgga cggtctgaat acgttttacc aacaatcttt acaacaacct gttgccacgt
    16561 ctgcgcgcca gtatctggaa aaacgcggat taagccacga ggttatcgct cgctttgcga
    16621 ttggttttgc gccccccggc tgggacaacg tcctgaagcg gtttggcggc aatccagaaa
    16681 atcgccagtc attgattgat gcggggatgt tggtcactaa cgatcaggga cgcagttacg
    16741 atcgtttccg cgaacgggtg atgttcccca ttcgcgataa acgcggtcgg gtgattggtt
    16801 ttggcgggcg cgtgctgggc aacgataccc ccaaatacct gaactcgccg gaaacagaca
    16861 ttttccataa aggccgccag ctttacggtc tttatgaagc gcagcaggat aacgctgaac
    16921 ccaatcgtct gcttgtggtc gaaggctata tggacgtggt ggcgctggcg caatacggca
    16981 ttaattacgc cgttgcgtcg ttaggtacgt caaccaccgc cgatcacata caactgttgt
    17041 tccgcgcgac caacaatgtc atttgctgtt atgacggcga ccgtgcaggc cgcgatgccg
    17101 catggcgagc gctggaaacg gcgctgcctt acatgacaga cggccgtcag ctacgcttta
    17161 tgtttttgcc ggatggcgaa gaccctgaca cgctggtacg aaaagaaggt aaagaagcgt
    17221 ttgaagcgcg gatggagcag gcgatgccac tctccgcatt tctgtttaac agtctgatgc
    17281 cgcaagttga tctgagtacc cctgacgggc gcgcacgttt gagtacgctg gcactgccat
    17341 tgatatcgca agtgccgggc gaaacgctgc gaatatatct tcgtcaggaa ttaggcaaca
    17401 aattaggcat acttgatgac agccagcttg aacgattaat gccaaaagcg gcagagagcg
    17461 gcgtttctcg ccctgttccg cagctaaaac gcacgaccat gcgtatactt atagggttgc
    17521 tggtgcaaaa tccagaatta gcgacgttgg tcccgccgct tgagaatctg gatgaaaata
    17581 agctccctgg acttggctta ttcagagaac tggtcaacac ttgtctctcc cagccaggtc
    17641 tgaccaccgg gcaactttta gagcactatc gtggtacaaa taatgctgcc acccttgaaa
    17701 aactgtcgat gtgggacgat atagcagata agaatattgc tgagcaaacc ttcaccgact
    17761 cactcaacca tatgtttgat tcgctgcttg aactgcgcca ggaagagtta atcgctcgtg
    17821 agcgcacgca tggtttaagc aacgaagaac gcctggagct ctggacatta aaccaggagc
    17881 tggcgaaaaa gtgatttaac ggcttaagtg ccgaagagcg atcgggaagc ccccgacagc
    17941 cgcactgaga ggcagcggca aatatataag tacgccctcg taattatcgt tggcggtaaa
    18001 caaccgttgg atttcagcgt taacggctga aggacatcgg gtcaatcgcc caacaccaac
    18061 ctcatgaaat aagtgtggat accgtcttat ggagcaaaac ccgcagtcac agctgaaact
    18121 tcttgtcacc cgtggtaagg agcaaggcta tctgacctat gccgaggtca atgaccatct
    18181 gccggaagat atcgtcgatt cagatcagat cgaagacatc atccaaatga tcaacgacat
    18241 gggcattcag gtgatggaag aagcaccgga tgccgatgat ctgatgctgg ctgaaaacac
    18301 cgcggacgaa gatgctgccg aagccgccgc gcaggtgctt tccagcgtgg aatctgaaat
    18361 cgggcgcacg actgacccgg tacgcatgta catgcgtgaa atgggcaccg ttgaactgtt
    18421 gacccgcgaa ggcgaaattg acatcgctaa gcgtattgaa gacgggatca accaggttca
    18481 atgctccgtt gctgaatatc cggaagcgat cacctatctg ctggaacagt acgatcgtgt
    18541 tgaagcagaa gaagcgcgtc tgtccgatct gatcaccggc tttgttgacc cgaacgcaga
    18601 agaagatctg gcacctaccg ccactcacgt cggttctgag ctttcccagg aagatctgga
    18661 cgatgacgaa gatgaagacg aagaagatgg cgatgacgac agcgccgatg atgacaacag
    18721 catcgacccg gaactggctc gcgaaaaatt tgcggaacta cgcgctcagt acgttgtaac
    18781 gcgtgacacc atcaaagcga aaggtcgcag tcacgctgcc gctcaggaag agatcctgaa
    18841 actgtctgaa gtattcaaac agttccgcct ggtgccgaag cagtttgact acctggtcaa
    18901 cagcatgcgc gtcatgatgg accgcgttcg tacgcaagaa cgtctgatca tgaagctctg
    18961 cgttgagcag tgcaaaatgc cgaagaaaaa cttcattacc ctgtttaccg gcaacgaaac
    19021 cagcgatacc tggttcaacg cggcaattgc gatgaacaag ccgtggtcgg aaaaactgca
    19081 cgatgtctct gaagaagtgc accgcgccct gcaaaaactg cagcagattg aagaagaaac
    19141 cggcctgacc atcgagcagg ttaaagatat caaccgtcgt atgtccatcg gtgaagcgaa
    19201 agcccgccgt gcgaagaaag agatggttga agcgaactta cgtctggtta tttctatcgc
    19261 caagaaatac accaaccgtg gcttgcagtt ccttgacctg attcaggaag gtaacatcgg
    19321 cctgatgaaa gcggttgata aattcgaata ccgccgtggt tacaagttct ccacctacgc
    19381 aacctggtgg atccgtcagg cgatcacccg ttctatcgcg gatcaggcgc gcaccatccg
    19441 tattccggtg catatgattg agactatcaa caaactcaac cgtatttctc gccagatgct
    19501 gcaagagatg ggccgtgaac cgacgccgga agaactggct gaacgtatgc tgatgccgga
    19561 agacaagatc cgcaaagtgc tgaagatcgc caaagagcca atctccatgg aaacgccgat
    19621 cggtgatgat gaagattcgc atctggggga tttcatcgag gataccaccc tcgagctgcc
    19681 gctggattct gcgaccaccg aaagcctgcg tgcggcaacg cacgacgtgc tggctggcct
    19741 gaccgcgcgt gaagcaaaag ttctgcgtat gcgtttcggt atcgatatga acaccgacca
    19801 cacgctggaa gaagtgggta aacagttcga cgttacccgc gaacgtatcc gtcagatcga
    19861 agcgaaggcg ctgcgcaaac tgcgtcaccc gagccgttct gaagtgttgc gtagcttcct
    19921 ggacgattaa tcggtaggcc ggatcaggcg ttacgccgca cccggcacta gtctctctgc
    19981 acaaac
//
LOCUS       DAJTQQ010000073        19585 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000073 DAJTQQ010000000
VERSION     DAJTQQ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19585
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.073"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..447
                     /locus_tag="OO309_002808"
     CDS             <1..447
                     /locus_tag="OO309_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR5847894.1"
                     /translation="QGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVLSLGIYD
                     EVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYELTSRNE
                     PFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            738..1466
                     /gene="mpaA"
                     /locus_tag="OO309_002809"
     CDS             738..1466
                     /gene="mpaA"
                     /locus_tag="OO309_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR5847895.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(1441..2406)
                     /gene="ycjG"
                     /locus_tag="OO309_002810"
     CDS             complement(1441..2406)
                     /gene="ycjG"
                     /locus_tag="OO309_002810"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR5847896.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            2525..3031
                     /gene="tpx"
                     /locus_tag="OO309_002811"
     CDS             2525..3031
                     /gene="tpx"
                     /locus_tag="OO309_002811"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR5847897.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3075..4616)
                     /gene="tyrR"
                     /locus_tag="OO309_002812"
     CDS             complement(3075..4616)
                     /gene="tyrR"
                     /locus_tag="OO309_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR5847898.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(4764..5825)
                     /locus_tag="OO309_002813"
     CDS             complement(4764..5825)
                     /locus_tag="OO309_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR5847899.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(5822..7219)
                     /locus_tag="OO309_002814"
     CDS             complement(5822..7219)
                     /locus_tag="OO309_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR5847900.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            7375..8373
                     /locus_tag="OO309_002815"
     CDS             7375..8373
                     /locus_tag="OO309_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5847901.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(8484..9356)
                     /gene="ompG"
                     /locus_tag="OO309_002816"
                     /pseudo
     CDS             complement(8484..9356)
                     /gene="ompG"
                     /locus_tag="OO309_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(9429..10510)
                     /locus_tag="OO309_002817"
                     /pseudo
     CDS             complement(9429..10510)
                     /locus_tag="OO309_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(10524..11183)
                     /gene="pgmB"
                     /locus_tag="OO309_002818"
     CDS             complement(10524..11183)
                     /gene="pgmB"
                     /locus_tag="OO309_002818"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR5847902.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11180..13447)
                     /gene="ycjT"
                     /locus_tag="OO309_002819"
     CDS             complement(11180..13447)
                     /gene="ycjT"
                     /locus_tag="OO309_002819"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR5847903.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(13444..14067)
                     /locus_tag="OO309_002820"
     CDS             complement(13444..14067)
                     /locus_tag="OO309_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5847904.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(14064..14486)
                     /locus_tag="OO309_002821"
     CDS             complement(14064..14486)
                     /locus_tag="OO309_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5847905.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSHLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(14508..15283)
                     /locus_tag="OO309_002822"
                     /pseudo
     CDS             complement(14508..15283)
                     /locus_tag="OO309_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(15301..16353)
                     /locus_tag="OO309_002823"
     CDS             complement(15301..16353)
                     /locus_tag="OO309_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR5847906.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(16384..17226)
                     /locus_tag="OO309_002824"
     CDS             complement(16384..17226)
                     /locus_tag="OO309_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR5847907.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(17213..18094)
                     /locus_tag="OO309_002825"
     CDS             complement(17213..18094)
                     /locus_tag="OO309_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR5847908.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18115..19398)
                     /locus_tag="OO309_002826"
                     /pseudo
     CDS             complement(18115..19398)
                     /locus_tag="OO309_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(19412..>19585)
                     /locus_tag="OO309_002827"
     CDS             complement(19412..>19585)
                     /locus_tag="OO309_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HCR5847909.1"
                     /translation="RIQRSNADGNCLTGLFNVSKNIQHVNITDLHGRDLISEVDILGN
                     EITLRPWQVMWIK"
BASE COUNT         4732 a         5413 c         4773 g         4667 t
ORIGIN      
        1 cagggacagg atattgtgta tgccaacctt acgggggaag atttagatat tcaggcaaat
       61 tcagtgatcg ctgcgatgaa agcctgtgat gttaaacgtc tgatctttgt tctttccctc
      121 ggtatttatg atgaagttcc aggaaaattt gtcgaatgga ataatgcagt tattggtgaa
      181 cccctaaaac cattccgcag ggctgcggat gcaattgagg cttctggact ggaatataca
      241 attcttcgcc ctgcctggct gactgatgaa gatattattg attatgagct tacctctcgc
      301 aatgaaccat tcaaaggtac gatcgtatca cgtaagagtg tcgcagcatt aattacagat
      361 atcatcgata aacctgagaa acacattggt gagaatatag gcatcaatca acccgggact
      421 gatggcgata agccattctt catgtaaatc aattatatag ataccatcgt actgtactat
      481 gtgcagtacg atttattaaa cttattaagg tgaactgatc aatcagatgc gattaacatg
      541 tgtagaacca gtaagaataa aatacgccat gtatataaca ttacgacaat tctaatatct
      601 gcacatcaat gaaaatgcaa acaacttccg gcaaaaaagt gatacaagat cgatgacata
      661 gcgttgaaga aaaggatttg ataatccgca aaatttgcac aacactgcct gtaatacttc
      721 ctgaaccaga gacgatcatg accgtaaccc gctcacgcgc cgaacgcggc gcatttccgc
      781 ccggaacaga acattacgga cgttcattat tgggcgcgcc gttaatctgg tttcctgccc
      841 ctgcagccag ccgcgaaagt ggtttgattc tggctggcac tcacggtgat gaaaactctt
      901 cggtcgtcac gctctcctgt gctctgcgga ctttgacacc ttctttgcgc cgccatcatg
      961 tggtgctgtg tgtgaatcct gacggctgcc agctggggtt acgggccaat gctaatggtg
     1021 tggatttaaa cagaaacttt ccggcggcga actggaagga aggtgaaacg gtttatcgct
     1081 ggaacagcgc cgctgaagaa cgtgatgtcg tattactgac aggtgacaaa cctggctccg
     1141 aacctgaaac ccaggcgctg tgccagctta tacaccgcat tcaacctgcc tgggtggtct
     1201 cattccacga tccactggct tgtattgaag atcccagaca caacgaatta ggcgaatggc
     1261 tggcccagtc gtttgaattg cctctggtaa ccagcgtcgg ttatgaaacg cctggctctt
     1321 tcggcagttg gtgtgcagat ctcaacttgc attgcatcac cgcagagttt ccgcccatct
     1381 cctccgacga agccagcgaa aaatatctct ttgcgatggc aaacttgctg cgctggcatc
     1441 ctaaagatgc aattcgcccg tcgtgaactg aagcgccggt tccacatcta ccgccagcca
     1501 ggtcggtccg tcaagatcgg cgaaactgac ctgcggcacc agcggtaaag cggcgctaat
     1561 ggcacgagag gtacacaaca tgcagcccag catcagactg aaaccttgtg cacgcgcttc
     1621 agtcgccagc gccagcgctt ccgtcagacc accggtttta tcgagcttaa tgttaaccat
     1681 ctcatagcgc cctttcagcg ccttcagatt gctacgagta tgacaacttt catcagcaca
     1741 aatcggcaac ggatgaataa aattctccag cgccgcatcg tcctgcgcag gaagcggttg
     1801 ttcaagcatc gcaacgccta aatccgccaa tagctggcaa cgcgccgcca acccttctgc
     1861 acgccaggat tcatttgcat caacgatcag cgtggcatcg ggcacagcgg tgcgaattgc
     1921 caccatccgc tcactgataa gatggttatc cagcttcact ttcagtaatt tcgcgcctgc
     1981 ctgccagagt gttgatgcac tattggccat ctgatcaggc gtaccgatga caaccgtctg
     2041 tgcagtgata actgtctcgg gaagcgttat gccgatcaaa tcagccagcg attgctgctg
     2101 ttttcgcgcc gcgagatccc acaatgcaca atccagcgca ttacgtgctg cgccggcagg
     2161 gagaattttt tgcaactcct cccgtgtcag ccctttctct aattgcggca cgacgctcat
     2221 aatttgcgcc attaccgagg catcactttc tccataacgc ggatacggcg tgcattcgcc
     2281 ggtgccttta ataccctctt cttccagttc aaccaccacc acgcgcgctt cactgcgact
     2341 tccccgggca atcacaaacg gggtatgtaa gggccaggct tcctcaaata ccttaacgat
     2401 tctcatcttt actccttgtt gtcgcggtgg tctgcggatt aagcgcaaat agcgtttgct
     2461 gtgttattga cagttagcat aaactaggtg tgacgttaac tatatgtaaa caggaaagat
     2521 aatcatgtca caaaccgttc atttccaggg caacccggtt acagtcgcca attccatccc
     2581 gcaggcgggt agcaaagcgc agacttttac tctcgtggca aaagatctgt ctgacgtcac
     2641 cctcggtcag tttgcgggta aacgcaaagt gctgaacatt ttcccgagta ttgataccgg
     2701 tgtttgcgcc gcatcagtac gtaagtttaa ccaactggca actgagatcg acaacaccgt
     2761 tgtgctgtgt atctctgccg atctgccgtt cgcccagtct cgtttctgcg gcgcagaagg
     2821 tctgaacaac gttatcaccc tctccacttt ccgtaacgct gaatttctgc aagcctacgg
     2881 tgtggcaatt gctgatggcc cactgaaagg tctggcagcg cgtgccgttg tggttattga
     2941 cgaaaatgac aatgtgattt tcagccagct ggtggatgaa atcaccaccg agccggatta
     3001 cgaagcagct ctggctgtac tgaaagcata agttaatatg cctgatggtg ttgcaccatc
     3061 aggcatattc gcgcttactc ttcgttcttc ttctgactca gaccatattc ccgcaactta
     3121 ttggcaatcg cggtatgtga aacgccgaga cgttttgcca gtttgcgcgt gctgggataa
     3181 ttgcgataaa gctgggttaa taccgagcgt tcaaaacggc tggtgatttc gtccagcgaa
     3241 ccttccatcg catcttcgcc cacggctacc gttgcggcgt cataatccgg caacaaaata
     3301 tcctgtggac gcagctcata accgtccagt tgtgtcagtg cgcgatagat agcgttcttt
     3361 aactgccgca catttcccgg ccacgcataa cgtgtaagta cagtattcag gtcagcggcc
     3421 agtttcggac gcggcacgcc ctgctcgtcg gcaaagcggg cgacgaacag ctcagttaac
     3481 ggcataatgt cctgcggaca gtcacgtagc ggcggcagat tgagcgtcaa cacgttcaga
     3541 cgataataga gatcttcacg gaacacgcct ttttgcacca gttcgaccag attcttctgc
     3601 gtagcgcaaa tcacccgcac atcgacatgc acctcatggt cttcgccaac ccgacggaaa
     3661 gtgccatcat taagaaaacg cagtaatttc gcctgcatcc gtggtgacat ttcccctatt
     3721 tcatccaaca gcaccgaacc accgttcgcc tgctcaaaga accctttctt cccttccgga
     3781 gcatgaccaa acagttcact ctcgaccgca tcttctggta tagacgcgca attcagcgcc
     3841 aggtaaggtt tgcccgctct ggggcttgcc tgatggcagg cgtaggcaaa aagatcttta
     3901 cctgtacctg tgtcacccgt aatcagcagc ggcgcgctta gcatcgccag tttctgcgcc
     3961 tgttcgacaa cgtgcttcat tttcgggctg acggcgacaa tttggctgaa ggcgctgacg
     4021 tcctgggcgg cgacattttg caactggcgg cccatacgaa tcgttgatcg caacatcacc
     4081 accgcaccgg tcaggacgtg ttgatcattt tcatcctgaa gataaacagg cgtaatctcc
     4141 atcaggaaat tctgcccatt aataacgaca tgctcgttat gcgaatcttg cggttcgctt
     4201 tccagccaac gtaaaaaatt aaagccgtta atcaattgtg cggcggtatg gttgcgcagg
     4261 cgatccaatt tttgcccaaa aagctgacag ctcgccgggt tcgccatatc cactttgctt
     4321 ttcatatcga cagagagcac aggttcaggc aacgcctcca gtaacgcgct caacgccaga
     4381 tgctcacgtt cggaaggcat ccacgggaca gtacgcacat cggtaacacc tgcaatacgg
     4441 cgtatttcgg ccatcagact gctgaaactc tcaaactcca gttcagcaaa attgaggtag
     4501 attcgcccaa tgggatcaat ctcaataccg cgtaaatcaa tgcctcttag cacgagtaga
     4561 tcgagtaatt cgcgggtcag accgagtcgg tcttcacaaa agacttccag acgcatggga
     4621 accttcacct gaaaaaagaa caattaatat gatgatatga cactatttgg atagcaggaa
     4681 ggtttctgtc aacaatcatt gacacaaaga cggaaaaacg ggcacttcgg cgtaaagata
     4741 tccggtcagc acccgatatt gcattatttt tcgctgggcg ttttgccttt ttgcagagtt
     4801 tctttcacct gaccgataag ctgacgacgg aaatccccaa ggcgaggttt gtcatcgtca
     4861 atccacggca gcgggcggca aagctccata gctttaatcc cgagtcgtac cgtcagaagt
     4921 ccagcaccaa ttccctgagc tgcgcgggta gacaaacgag cagcgagatc ttgcgacatc
     4981 cagtccatcc ccacttcgcg caccagttcg ctggctccgg caaaagcgat attcagcaat
     5041 accagcttaa acagacgcaa acggctgtaa taccccagtt caatgccata cagcgtggca
     5101 atgcgattaa ttaaacgcag attgcgccag gcgataaacg ccatatcgac caacgccagc
     5161 gggctgaccg caatcatcaa cgttgattcc gccgccgaac ggctgatttc gcgccgcgcc
     5221 tgggcatcta aaactggctg gaccaaatgc gcatacaggc tgaccacttc acgatcgttc
     5281 tgcgtttcat ggattgaggc ataccagcgt tgcagcgccg gatgcgactg atcaataccc
     5341 gcctgctgcg ccagtttttc gcaaaatgcg cgacctttgc ccgtgccgtg gctatgcaat
     5401 aaatcacgcg cttcgtcgcg ttcatgggcg cgctgtcgca agcgccataa gcgccgccac
     5461 tctgttacca cagaacctac gccagcgccg ataatcaatg ccccagcggc acatccaccc
     5521 agcgctaccc agtcctgggt ttgccaggca ttcattgtcc actgtatacc ctgcccgaca
     5581 acgcttgcgc caaacagagc cagcccgccc atcaccattt tgcgccacag gctgcgtttc
     5641 ggacgtaacg ctgcgtccat taccgcttcg acttgcccct cttcttcctg cgcttcgtcg
     5701 agcgtggccg gggcaaaatt ttgcgcctga ttttcgtcaa aggtctgctg cgccctgaat
     5761 tttggattct gttcgacctc cagcggaccg tcgaaatcaa tacgtggttt taacggttcg
     5821 gtcatcgcaa tttatctcct attaaaaatt ccagcgcggc atcaagacga atatgcggca
     5881 gcggtttgtc gacatccatc acctgcgggc gaaacgcttc aaactggaac ccttgcttgt
     5941 cccagaacgc ctgaccgggc aaacgcgcag gaacttcacc ggggtaaacg gtgagaagcg
     6001 caccatcgct gagccggtta ccgcgcaatg ccggaatttt ctcgccgttg acgtcaataa
     6061 tgccgctggt ggtcgcctgc accgaggcca gccccaggca atccatacta atcctttcaa
     6121 acgccgcatt ttgccaggca tcctgaatca gttgttgcag taatgaaacc atattggcgt
     6181 gctgatcgac cgttacatgg tccgctttca tcgcggcaaa cagcagtttg tcgataaccg
     6241 gcgagaataa gcggcgaaac agcgtgcgct gcccgtagtg gaaactttgc atcagctgcg
     6301 tcagtgccag acgcatatca ttaaatgcct gtggcccact gttgagaggt tgcaggcaat
     6361 ccaccagcac aatctggcgg tcaaagcgca gaaaatgatt cttatagaac cccttcacca
     6421 ctttctcgca gtaataatta aaccgctcgc gcagcattcc ggcattggtg tgcttatcgg
     6481 cctgcgccag tttggactcg tcccaggcat cgacatccgg ccacgggaag aattgcagcg
     6541 cgggcgcacc tgccatatct cccggcaaga caaagcgccc aggctgaata aagtgcagcc
     6601 cctgctgttt acagtggtgg agataatcgg tccacacggc ggcaatgtct gccagacggt
     6661 tttcgtcggc aggcgctagc gggtcaagtc cttcgcacat cattcgccat ttcgccgacc
     6721 attcgccgcg ctgaccattg agtaagcccg tcatctggcg cgaccagctt aagtaatcct
     6781 gcgccagcat cggcaggtcg agcaaccatt cgccgggata atccacaatt tccagataca
     6841 gcgtggaggt atccttaaag tggcgcagca gcgcatcgtt cgatttaaaa cgtaacgcca
     6901 ggcgaatttc actgacgccg cgcgtaggcg tcggccaggc gggaggatca ccatataact
     6961 gcgccagtcc ttcatcatag gtgaagcgcg gaatgccaaa gtcacgctgg ggaatgcgtt
     7021 ttacgcccag caagcgctct tcacgcaccg cgcttaacag cggcaaacgt gctccggcat
     7081 gaatattgag caactgatta accatcgcgg tgataaacgc tgttttgccg ctgcggctaa
     7141 gtccggttac agcgaggcgc agatgtctgt cgacaccccg attcaccagc gcattgagtt
     7201 catttttaag tcgcttcatt gctgtcctgt gctgctctga agaccagaaa aaaaatcatt
     7261 cgtaaggata tgaatgtggg ggccaggcca ttgattatca actgcccgtg ttgtcatcga
     7321 cgggcgaact ccataagatg tgcagcattc acgttcggac ggagttcctg acctatgtcc
     7381 cctactattt atgatattgc cagggttgca ggcgtatcaa aatccaccgt atcacgcgtg
     7441 ctgaataagc aaactaatat ctccccggaa gcgcgcgaaa aagtgttacg ggccattgaa
     7501 gaattacagt atcagccaaa caagctggcc cgcgcgctga cctcttcggg ttttgatgcc
     7561 attatggtga tttctacccg ttcgaccaaa actacggcgg gtaatccgtt tttctctgaa
     7621 gttttgcatg ccatcaccgc caaagcggaa gaagaaggtt tcgacgtgat attacaaacg
     7681 tcgcacaatc cggcagaaga tctgcaaaaa tgcgaaagca aaattaaaca gaaaatgatt
     7741 aaaggcatta ttatgctcag ttcgccagcg gatgagtcat tttttgccca actcgataaa
     7801 tacgatattc cggtggtggt aattggcaaa gttgaaggtc aatatgccca tgtttattct
     7861 gtcgataccg ataattttgg tgacagcatt gcgttgaccg atgcattaat tgaaagtggg
     7921 catcaaaata ttgcctgcct gcatgctccg cttgatgtcc atgtttcggt tgatcgcgtt
     7981 aatggctata agcaaagtct gtccgcgcat aatattgcag tgcgtgataa atggattgtt
     8041 gacggcggtt atacccatga aacagcactg caagcagcac ggcaattatt aagtcagtca
     8101 ccgttgcctg aggcagtgtt tgccactgac agcctgaaat taatgagcat ttatcgtgcg
     8161 gcggcagaga aaaatattgc tattccgcag cagttagcgg tggtgggtta tagtaatgaa
     8221 acgctgtcgt ttattttaac gcctgcacca ggcggcatcg atgttccgac gcaggagtta
     8281 ggtcaacaaa gctgcgagtt attattccgc ttaatttcgg gaaaaccgtc accacagaat
     8341 attaccgttg ccacgcatat gacgctgaaa taaataccag ctaccttgcc tgaatgccgg
     8401 atgcgatgct ggcgcatctt atccggccta catttataga tcggataaag tactcgcgcc
     8461 gcatccggca atttagccca ttatcagaac gagtaattta cgccgacacc tgcataatgg
     8521 aatttatcgc tgtcgccttc gtcgtgatcc tgccactcaa acgcgtattc cagcgaaacg
     8581 gaaagtccgt tctggaaatc ataaccgtaa aataaaccca cacggttaaa atcatggcct
     8641 tcacgttcaa tatcatcctg ccagtcccag ttactctagc gatccagccc aatgcgcgta
     8701 tacggcgtca ccgagtggtt gccgagcgtc agcggcaaat aggcgcgaat ttcttgtgtg
     8761 gaaaactcac cgttattgcg gctgtcgtcc atattgaagc cgcgctcgag ataatagttc
     8821 actcgcaaag caaccgtttc gttgaaggta tattgcagac ctgtttccgt ttcgacacgg
     8881 gtatcagcgt aaccggtcgt gttcaaatcg ttggcaaatt tatacatcga caaccaaccg
     8941 ttgaaacgta aatcatcagt cagtttcaca tcccagtctg gcgcgatttt ccagcgctgc
     9001 atattcgccg tgtctttgcc cggttcatca acgtagtgat aaccataatt acggaaaccg
     9061 ccggtcaggc cgaaactgaa atcatcgctt tcgaggaact gataatgcac ctccagctcc
     9121 gggcgatcaa acctcgttcc acgtttaccc gcactataat ctaccggccc ttcctgataa
     9181 taggccagag caattctcca cggcccgttg gcggcattaa aatagactga aggctccgcc
     9241 agcccatcca tatcttcgcc ataaccctcg acgttttcta tttcgtacat cgcaccgata
     9301 ttaaagtgcc agtcgctctt ttcctcggcc tgtgcgcagg ccattcccgc acacatcacc
     9361 agtgcggtac agggtaataa ctttttttgt tccttgtatt atcctggggg aatgccttcc
     9421 ccctgtattt agcgaattgc tatttccgtt tctgcatcaa agaaatgaca tttcgtcata
     9481 tcaaaatgaa tagtgatatt ttctcctgca tgataatcat ttaacgcacc agcacggacc
     9541 actaactcgt gcccccaacc gtggtgtaga gcataaattc cgcaccggtt aattctgcca
     9601 cgctaatttt ggcggaaatg ttattttcct cttgcgcgtc cggatgaata tcttccgggc
     9661 gtattcccat cacgatgggc ttatgcaaac tttcctgtgt tttcagaacc gctaatttct
     9721 cttcgagaat ggttaattta agcgtttccg taacgaattt atcgccatcg atcgtgccgc
     9781 gaataaaatt catcgctggt gatccaataa atccggcaac aaacatattc gcgggttggt
     9841 tataaacggt tttcggcgca cctacttgct gaacaatccc gtctttcata atcacaatcc
     9901 gcgccgccat ggtcatcgct tcggtctgat cgtgggtcac gtagatcatg gtggtgttca
     9961 gtttctgatg cagcttgctg atctctgcac gcatttgtac gcgcagcttg gcatcaaggt
    10021 tagagagcgg ttcatccatt aaaaacacgc ccgcttcgcg cacaatcgcc cgcccgagcg
    10081 ccacacgctg gcgttgcccg ccggaaagcg cgcccggctt gcgtttcagg tactcacgca
    10141 ggccgagaat ttgcgccgcc cagttcaccc gctcatcaat cacctctttg gcgatttttt
    10201 gcatcttcag gccaaacgcc atgttgtcgt aaaccgtcat atgcggatac aacgcgtagt
    10261 tctggaacac catcgcgata ttgcgtgctt tggctggaac gtcattcatt cgtttgccgt
    10321 cgatcaacag atcgccgccg ctgatctcct caagcccggc aatcatgcgc agggtggtcg
    10381 atttaccgca gcccgacggg ccgacaaata cgatgaactc tttatcggca atttccaggt
    10441 tgaagtcctt caccacatgc acctggttat cgtagatttt ttgaatatgt tgtaacgaaa
    10501 gctgagccat gttgattcct ttgctatacg ttttgccaga aggccgataa ccgcgaccag
    10561 gtcagggagt ccgttgaggg caacagtaat tgcgccccgg ttaagcccgc accgatcccc
    10621 accgagcgca taccgctggc gttaatggcg tcaatgcccg cctgcgcatc ttcaatgccg
    10681 atacatgcct gcggcggcac gcccagcccg gcacaggcag cgagaaagat ttccggatcc
    10741 ggtttcgagt ttttaagttg ggaagcatcc gcgcagaagg tgaaaaactc acgcagctcc
    10801 agcgccgcta aaatcgtcgg cgcattcagg gagacggaag ccagccctac cggaatctgc
    10861 tgtgcacgga gctctgccag caaattgcga ataccgggca gcacagcatt aaccgtcagc
    10921 tcgcgcagtg agtggacgta aagcagattt ttgcgatacg ccagttgcgc ccgctcctgc
    10981 gggttaaagt cgccctcttt gcccccgtgt tgcagaatgc gccgcagaga ctcatcgcgg
    11041 ctgatccctt ttagagattc gttaaactgc gcatcaatac tgatgccaat ttcagcggca
    11101 atctgctgcc acgcctggaa atgcagatgc gcggtatcgg tgattacacc atccagatcg
    11161 aaaattaccc cttgcagttt cattcatcct cctgatgcgt ggtagcggtc ccattgaagg
    11221 gcaaaataaa atcacccaaa cagaaaacag attcttcagc cacggatata agctgaccgt
    11281 ccaaacgcag tgaaacgggc gcagaagttc gaatcgcaat acgctgcgcg tcgagagtga
    11341 cctgtaattc gcagccctgc cagaacagag ggaaagacaa ctgttgccac tgctcaagta
    11401 acgccggatt tagatgcaat tcaccgtcac gcacgctcac cccggcaaaa ccctgaatcg
    11461 cccccagcca gatagcgcca gttgcggcag catggatacc atcatcacaa ctatgcggat
    11521 cagcaccaag atcgatttca gtcccctcgc gccagaactg ataactttgg gtcagcaggc
    11581 cacagcgtgc agcaacaatg ccgtggattg ctttacttaa tgacgagtcg tgaatagtgc
    11641 gcggttcata aaattgcaga ttggcaagac acgatgccga tgagaactgc tctggcagca
    11701 tgtaattgag catcaccaca tcagcttgtt tgaggatctg catctcgttc acttctgcgc
    11761 gtgaataatc cagcagaatg gtttgcttcc ccgccgccgc tttgtatttc gccagattaa
    11821 tcgccggctt agtcataaac gaatcatcct gcggcaaaac accgtcgggc tgaatttctg
    11881 gcagccgcag ttctttaagg aacatttcgg cacgatgaat aaacgcgtcg tcactacagc
    11941 caaactggcg ggcaatactc agcgcctgct ggacgttgta atacaccata tagctggtga
    12001 aggcgttatt attgacatgt tcggtatatt cgtctggccc aataacatca tgaatttcca
    12061 gacggtcgtt aaccctcacc gcgcggctaa tccagaactt tgcagtttcc agaagtagcg
    12121 ccatgccttc atgcgcaatg aaactttcat cccccgtggt ctgccagtat tgaataaccg
    12181 cccaggcgat atcggccacc agatgatgtt ccgcctgcgc cgaggccact ttttgccgca
    12241 gcccggtgcg aatgttaatg gcggcaaatt ccggcgtctc ttcttcgccg ctgcgcgcgc
    12301 tttcccacgg aaatagcgcg ccctgccagc cgttgcgtcg cgctttctcc tgcgcgcctg
    12361 gcaagttgtg ccagcgataa cgcagtaaac ttcgggcaac cgtcggatcg ctaaacagat
    12421 ggaacggcag caaaaatact tctgtatccc agaaaacgtg gcctttgtag ccttcgccgg
    12481 ttaagccttt tgccgcaata ctgctgcgct cgccgtgagc cggcgtcatg atgcgcagat
    12541 gataaagcgc ataatctaac gcttgctgat cgtgcgcatc gccgccattt accgtgatac
    12601 ggcgtttctg ccaccattga cgccagtttt ctgttgatgc tgcaagatgt tggtcgtaac
    12661 tctgttgcgc gcacatttca agctggcgaa gcgacacgct gccccactcg tctaaagcag
    12721 cttgcctgtc atcccgccag tcgatccaca ccagtttttg caacgtcact gtctcgcctg
    12781 catgaagctg cgtactggta tgttgcagta aacggcgctc tttggcggta tagcattgct
    12841 gcacatcacc gctcacctgg cagcaacaac tgatggccac atcactgcgc ccatcctggg
    12901 tggtgtagat cccctgcatc aaatgctgac caaacacccg cacctgggtt tcgtcgagat
    12961 gctggcgacc gtggttggtt tgcgtggcgt caatgcctgt tgaaatcagc actgacgcgt
    13021 cggcgtccag tggcataata gtgatttcca gcgcaatgag cggcagttgg tctgccgaaa
    13081 caaagcgacg gctggcgatg gtgtaacctg cgccgttgct ggtacgccag acaacgctac
    13141 ggcgtaattc tccactggca aagtcaagtt cacgctgcca ggcttcgcgg gataacgaga
    13201 aaacctcacc atttatggca atctccatcc ccaagatatc aggcagattc accagttcgt
    13261 tgatttcacc ttttcccgcc cgatgataca gccccgccag atacatccct cgcgtctggc
    13321 gcgtgtaatc ttcttcatgg ctggcgcgaa gcccaagata gccgttcccc tgcgccatca
    13381 gcgatgcata cttgttcagg gtatgctggc tgaaatgggg ttctgataac gttactggcc
    13441 tggtcataat tccacacgcg tccctgtttc tgcggattga tataacgccg ccaccagttg
    13501 ctggatgatg tacccctgct cggcgtcggc tatcatcacg ggcttgccct gtacgtgatt
    13561 gataaaggct tccatgctgc gcaaatggcg gttgtcgtct gccatttccc gttgcatcag
    13621 cgtcattaat tcaccgttgt tatcggtgta gatatgtgct ggaaacagcg tcgcaccggc
    13681 tttatcacca caaaagctga cgttcataat caactgttcg cggatgttga gtgcaaatga
    13741 cgtttccagc cacagaatgc cgccgttatg aaattcaatg gtgccaaaca gcgaatcttc
    13801 gacgctgtaa gttgccggat cccactcgcc aaattgacca cagctctttt gcgtgccgat
    13861 cttttgaaaa ctatgcgcat tcacgctttt caccgccgga aagcccagca catacatcgc
    13921 agcatccagc atatgaatgc cgatgtcgat cagcaggcca ccaccctgca gttctttatt
    13981 ggtaaagaca ccccagccgg gaacgccgca gcgacgcagg gcgcgggcgg tggtaacgta
    14041 aatctctccc aaaacgccgt tggtcacctg ttcacgcagc tgttgcgtat cgagcgcaaa
    14101 cgatggtgaa agtcgtaggc cagcaccttg cccagtttgc gcgcggtatc gcacatttcc
    14161 cacgcctgtt ctgtcgtcat ggcgggcggt ttttcgcaca tcacatggca gcctgcttcc
    14221 agtgccatca gggtatgttc gtaatgaaaa cggttaggtg agccgacgct aaccacatca
    14281 ggtttcaccg ccagcagcat ggcctgcgga tcgtcccaca cggatgcatt cccgtatttt
    14341 tctgccagcg cctgcgcctg ggaaaggtgg ctgtcacaga cagccaccag ttccagatcg
    14401 ttgcgggtgc agtagtacga agcatgaacc ttatccgcca cctggcccgc gcctattatc
    14461 gcgacccgca gcggagagct tgtcattgca cttttcacaa agacctctta gcaggtacgc
    14521 aaccaggcca acgaatcacg gtacgcctgg gcaggatctt ctgcccggat acgcccttca
    14581 tacaccacat aaccctgata gttatcggcg cgcagctgtt caaacagcgc gtggaaatcg
    14641 agggtgccac tgcccggctg gtaacgatgg ttatcggcaa tatgcacatg accgagcaag
    14701 tcacggttgt cgtgcagcgc ctgcgccagg ttatcttctt cgatgttcat gtgatagaaa
    14761 tcgccgataa tctgcacatg tttaagattg ttttcgacga tgtaacgacg agcatcggcg
    14821 agggtgttga tcatatgatc ctgatagcgg tttaacggtt cgagatacac cacggttccg
    14881 gtacgcgcgg cgacctgttc cagtacgcgc agggaatcac tcaccagttt gcggtcgccg
    14941 tccaggctac gcggcgaggt catcggcggt aagcggaagg taaacatgcc ccacgctgcc
    15001 ggaacccccc cacttctgcc agcgcctcga gaatgcgttc gatctgcttt aagccattaa
    15061 gacgacgctc ttcgataaag tcaccaatcc agccgtcata gccgccgcag gcggtggtca
    15121 ctggtaaacc ggtttctttg atcgccgctt tgacttcttc gatgttgttg accagcagtt
    15181 tgccgtcaat ctcaaaacca tcgaagccca tctctttgat ataacgaaat ctctcgcgga
    15241 tattttccgg aaagaatgcc tgattctgtg tgccgatttt cattttttca ttcctttgaa
    15301 ttaaaacgta acgcccattt tgatgctctg ttccggatgc tgatcgacat actgcatgta
    15361 gctttccggg ctgttggcaa aggtcactac cgggtcgatt aaatcttcgc aattgagata
    15421 accgttcatc agcagttccc agcaggtttc ttcaatacgc ttacggctcc agcgcggata
    15481 atccgggttc ggttcgctgc acgcgcggga gaaaacaatt ttggcgttat tgaaatgcgc
    15541 ttcgcgtccg aggttaaaac cttcagcaaa tggtttggca aacgcgacat aggagatggt
    15601 gccgccgtag gccaggccgc gcagcgccga ttgcagcgcg tcggcgtaac cgctggtttc
    15661 gataatcaca tcagccccct gcttgccggt cagcgttttg atctctttgc ctacgtcagt
    15721 gccaatgggg ttaaggcaga aatcagcacc gtggcgacgg gcaatatcac agcgatgggc
    15781 gataggatcg acgccaatta ccaccgatgc gccagcgcgt ttcgccagtt ggatggcaat
    15841 ttgaccgatc gcgccaagcc ctaccaccac gacaaaatcc cctacgcgta cgttggcatc
    15901 gcgcacgccg ctcatggcaa actgcgccgg gtcgtaacag acggcgtttt tccaggagct
    15961 gccttgcggc attttgcgca gcttgtagtt attcactgcg ttaatgatga ccgtctcgga
    16021 gagcgggccg tagccgcata cgctgtcgcc caccgcgtag tctgtaacgt cgctgccgca
    16081 ctcgataatg tcgccaacca ccatgttgcc aagctggaat ttgccaaact caatgccgcg
    16141 cggcgcatct gccggacgcg gggtgaacat ctgccactcg ccgttaaagt cttcatcgat
    16201 aaacgggctg gcggcgcgga agtcgaccac ttccgttccg tgtttcggtg cgccgaaacg
    16261 cacgcggatc ttcacttcat tggctaaaat cgcccgatct tcatactcaa ccagcgcagc
    16321 aacacgcggt gctgtggcta ctaacttttt catgtaaaac tccttgtaaa gaaactggcc
    16381 gctttagccc ttcacgccac cggcggtcaa accactctta ataaaacgtt ccaaaagcgc
    16441 atacataatc accaccggca atgcggtcac cagcgaggcc gccatcatcc gtccccagat
    16501 gtaatcaggc gtactgaaca gcgcgttcag gcccactggt aaggtgaaat tgctggcgct
    16561 ggagaggaaa atcgacgcaa acagatagtc gttccacgcc accataaagc agtagacgaa
    16621 caccgatatc agcccggaca tcgccagggg cacggtgata cggaagatga tttgcagacg
    16681 gttgaggcca tccatcatcg ccgcttcttc gatctcatca gggatggtgt cgaagtaact
    16741 tttcagcata aacacggcgg tgggcagggt ttgcgtcacc atggtaatga tcagcgccat
    16801 ctcggtgtcg taaataccaa gcgcggtgat gattttgaac agaggcacca ccagcaaaat
    16861 gccagagaac atatacaccg tgtaaaagct ggcgttgatg gtcatccggc ctttaaagcg
    16921 caggcgggaa agcgcgtaag ctccgagaat gccgagaaat accgccacca cggaggaaac
    16981 caccgacacc accatgctgt tacggaagta gtcgacaaac gggaaaatca tcgggttaaa
    17041 aatgtcgacg taatgctcca gcgtccactg ttgcggcagc agcgtaggat gcagcgatat
    17101 cgcctctttc gcgcttttga acgaggtcat cagcatcaca aaaaacggaa acagcgtgat
    17161 gatgagaaac agcgccagcc cgcagtaaaa accgatacga ctgagcgtgc gtttatttgt
    17221 tgccattgag gttcacccgt ttcctggtca gcagaatgac agcgaaaata atgatgaaga
    17281 gcaccaccga gatcgccgcc gctttcccta agtcattaaa ggcaaaggcg gttttgtaga
    17341 gatagacacc aagaatatcg accttggttg tcagcagata aacatcagcg aacatgtaga
    17401 acatccagat ggtgcgcagg gtcaccaccg tcgccaggac cggcataatt gcgggcagcg
    17461 tgacgatgcg aaaccgctgc caggcattag cgccatccat ctctgccgct tcgtacagcg
    17521 atttgtcgat ggtctgcaaa atcgccagaa acgagataaa ggcatacggg aagtagcgcc
    17581 agatggcgaa cagcaccacc agcacaaagc tactgcccgg attatcaaac cacagcggtg
    17641 cctgctcata gagatgcaga agatcgacgc cgaggtagtt cacaatgccg tagccgttgt
    17701 tgaacatgta tttccaggca aacaccagcg aaatggacgg cgttacgtag gagaggatca
    17761 ccagcgaacg cgcggttttg cgcaggcgga actcgcggtt gaaaaacatc gccacggcca
    17821 gcccgagcac ggtgctgccc gccaccacca gcgccgtata ccagaccgtc atccacagcg
    17881 agtgccagaa gccgggatcg gagagaatgc gcacatagtt actcacccca acaaacgttg
    17941 actcgatgtt gggattgagc ggcagacgta aaaaactgat ttcgatattc gacaccatcg
    18001 gccacgccac cagaccgccc agcagtaata agctgggcgc gagaagcagc agcgcaaacg
    18061 gcatatcgga acgacctgaa aaaagcctgt tcattgcgga ttcctgtcct gggtttagtg
    18121 ctgttcgatc agttcatcca gctttttctg gctcgcttgc agcgtgccag ggagatcggc
    18181 tttacccacg gtgacgttat gcaccatgga gctcaccaca ccagaacccg tcacatcgcc
    18241 catgcgggta aagtttttat cccctactgc gccaaagacc tgaatatttg gcagttcacc
    18301 gattagctga ttcggtagtt cacccagcgc cttaataacg tcgttgtctt tccaggtggc
    18361 ggtagtgaca accgctttat tcaccggcag cgcagcacct ggcgacatca tcacccagtc
    18421 ggcaatgttg tccgcctgct ccataaaggt gacaaatttt tctgctgctt cggtcttttg
    18481 tccggcggta atggtcagcg aggtcaacat gccgtagacc gcagagtttt tctcggttgg
    18541 caccacgaaa ccgacgtttt tcgggtcgcc ttctttaatc acagccggaa ggatataggt
    18601 ggagtaaatc gccatcggcg cggtgccgtt cataaaggcg tctttcactt ccatgatgtc
    18661 gttagaaccc ggcatggtgt tggcagcaag gtcgcggtaa taggtcagcg cctgcatcat
    18721 ctctggtgta tcaagggtga ttttgccttc ggcgttaaag acgttggcct agttggataa
    18781 cgcaaactgg gagaaggatt gttccgtcag cacgctttct gccgtaggca gcgcaatacc
    18841 gtatttttta ttcgccgggt cattcagttt ctgtgcaacg tccagcagct gttgccagtt
    18901 tttcggctcc tccagccccg cttttgccag cacatctttg cgataccaga tgccgccaat
    18961 ccaggcgctg acaggaacac cggtccatgc gctaccatct tcggtacgca caatacgcag
    19021 tacgccatcg taaaacgcgc cttcaccaac gttgctgatg accgtggcaa ccgctttgcg
    19081 atcgataagc tgctctttat ccataacttt ggcgtagtca tggctggttt cgatcacttc
    19141 cggcagcgag ccgctacgtg aaagggtgat gactttggtg ttatgagcgt cttcttccac
    19201 gggcacttgt ttaacggtga tacctgggtt ttctttttca aaacgggcaa tcaatttgct
    19261 gataactgcc tgacgctcct gctccaccga agaatgcata aattcgatgg atacatgggt
    19321 tttattttct tctttacagc ctgaaatcag ggcgcaggaa accagtgctg ataacagcac
    19381 gatttttgat ttaatcatga gatgttcctt tttatttaat ccacataacc tgccaggggc
    19441 gcagcgttat ttcattaccc aatatatcaa cttcactaat cagatcccga ccatgcagat
    19501 cagtaatatt tacatgctga atatttttac tgacattaaa caatccagtc agacaattac
    19561 catcagcgtt acttctttga atacg
//
LOCUS       DAJTQQ010000074        19487 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000074 DAJTQQ010000000
VERSION     DAJTQQ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.074"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..554)
                     /gene="aroD"
                     /locus_tag="OO309_002828"
     CDS             complement(<1..554)
                     /gene="aroD"
                     /locus_tag="OO309_002828"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR5847910.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLL"
     gene            complement(585..1451)
                     /gene="ydiB"
                     /locus_tag="OO309_002829"
     CDS             complement(585..1451)
                     /gene="ydiB"
                     /locus_tag="OO309_002829"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR5847911.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1463..2735)
                     /locus_tag="OO309_002830"
                     /pseudo
     CDS             complement(1463..2735)
                     /locus_tag="OO309_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(2918..4123)
                     /locus_tag="OO309_002831"
     CDS             complement(2918..4123)
                     /locus_tag="OO309_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5847912.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4223..4579)
                     /locus_tag="OO309_002832"
     CDS             complement(4223..4579)
                     /locus_tag="OO309_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCR5847913.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4715..4822)
                     /gene="rprA"
                     /locus_tag="OO309_002833"
     ncRNA           complement(4715..4822)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO309_002833"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5008..6120)
                     /gene="ydiK"
                     /locus_tag="OO309_002834"
     CDS             complement(5008..6120)
                     /gene="ydiK"
                     /locus_tag="OO309_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR5847914.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6509..9565
                     /locus_tag="OO309_002835"
     CDS             6509..9565
                     /locus_tag="OO309_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5847915.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9562..9972
                     /gene="menI"
                     /locus_tag="OO309_002836"
     CDS             9562..9972
                     /gene="menI"
                     /locus_tag="OO309_002836"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR5847916.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10072..10260
                     /locus_tag="OO309_002837"
     CDS             10072..10260
                     /locus_tag="OO309_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR5847917.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            10808..11176
                     /gene="sufA"
                     /locus_tag="OO309_002838"
     CDS             10808..11176
                     /gene="sufA"
                     /locus_tag="OO309_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR5847918.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11185..12672
                     /gene="sufB"
                     /locus_tag="OO309_002839"
     CDS             11185..12672
                     /gene="sufB"
                     /locus_tag="OO309_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR5847919.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12682..13428
                     /gene="sufC"
                     /locus_tag="OO309_002840"
     CDS             12682..13428
                     /gene="sufC"
                     /locus_tag="OO309_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR5847920.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13403..14674
                     /gene="sufD"
                     /locus_tag="OO309_002841"
     CDS             13403..14674
                     /gene="sufD"
                     /locus_tag="OO309_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR5847921.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14671..15891
                     /gene="sufS"
                     /locus_tag="OO309_002842"
     CDS             14671..15891
                     /gene="sufS"
                     /locus_tag="OO309_002842"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR5847922.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            15904..16320
                     /gene="sufE"
                     /locus_tag="OO309_002843"
     CDS             15904..16320
                     /gene="sufE"
                     /locus_tag="OO309_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR5847923.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16469..17473
                     /gene="ldtE"
                     /locus_tag="OO309_002844"
     CDS             16469..17473
                     /gene="ldtE"
                     /locus_tag="OO309_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR5847924.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17536..17772)
                     /gene="lpp"
                     /locus_tag="OO309_002845"
     CDS             complement(17536..17772)
                     /gene="lpp"
                     /locus_tag="OO309_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR5847925.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18083..>19487)
                     /gene="pykF"
                     /locus_tag="OO309_002846"
     CDS             complement(18083..>19487)
                     /gene="pykF"
                     /locus_tag="OO309_002846"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR5847926.1"
                     /translation="TKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHGQRI
                     QNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGNSEM
                     VAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNLPGV
                     SIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHIISKI
                     ENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVITATQ
                     MLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICERTDR
                     VMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFPDAT
                     ILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVVMVS
                     GALVPSGTTNTASVHVL"
BASE COUNT         5090 a         4712 c         4992 g         4693 t
ORIGIN      
        1 agcaacgtca gcacatcgct ggtactttgc ggcatcagcg caatcttagg aatatcggcg
       61 tcgaaggact gcattttgcg cagacgggca atgatttctt cggcttccgg cgttttatgg
      121 aagtcatggt tggacatgac aactttcaca tcatgcgcgt gggcgtaggc gacggtttct
      181 ttgacctgat catcgccggt aaataactcc agatcgatca tatcaaccag gccgctgtcg
      241 atggctgcac gattgagagc aatataagcc tcggtggaaa tcgcctgctc gccgccttct
      301 ttggcactgc ggaaggtaaa cagcagcggt ttttctggca tggtttcacg gagaattttt
      361 gccgccgcca tgacagactc cacattggag aggtcggcaa agtggtccac acgccattcc
      421 agaatatcaa agtccgcttc acgataggcg agagcttcgg atttcacgcg ggcgatatct
      481 ttcgccatca gcgagacgat gattttaggt gcgcccgcac caatgacgag atcttttaca
      541 gttacggttt tcatttttta ccctttctgc acgcggtcag cctgtcaggc accgaacccc
      601 atgacctgtt taacatattc cagagggaaa tctttgccag tccataacgt gaactgttca
      661 gccccttgcc acaacaacat gccgtatcca tcaatcgttt tgcaaccagc ttgttgtgcc
      721 tgctgcaata acttcgtcat atgcgggtta tacacgcatt cagtgaccag cagtcccgga
      781 tgtaacagac tgatatcatt aaccaatgat tcattctcaa ggggtttcat acctactttt
      841 gtgccattgg ttaaaatgtc ggcggaagcc agggcttcag caaaggcttg ctgatcggcg
      901 agatcggtat ccgtgacgac acaatcggtg ttttcgttaa cccgctgcgc gaaggcgagg
      961 gctttatcga agaactcatc ccgacggtta aagagtttaa tttcttttaa accttcaatt
     1021 gccccctgcg cgccaatcgc cgttgaggca cctccggccc ctaacagcac catcgttttg
     1081 cctttgatat caaaaccgct ctctttaatg gcgcgaatat ggcccgtgcc gtcggtgtta
     1141 tagccacgca gatagccatc atcattaacg atggtgttga tggcacccac cagtttggct
     1201 gctggcgtta attcatcaac atattcacac gccagttgtt tgttcggcat cgacacacca
     1261 gttccgcgca ttttgagggc ttttaatcct tcaattgctc cgggaaagct atcgttatcc
     1321 acttcgaagg ccatataggt aaatggcaat cccgcttttt ctaaggcttt attctgcatt
     1381 tcaggcgata aactgtggcg gataggataa gccatcaatc caatcaattc gtattttgcg
     1441 gtaacatcca taattaactc ctttaacctc tatgcttaat tgtgtttaac cgggtgctaa
     1501 atttgcgttc gccaaaccgc acatcattct caggaataat aaaaacgcgg taatagcggc
     1561 taaaaacaat aattgcggta atcagggcca gcagcgcgaa agtaaaatcg agaacaatga
     1621 tatattgcag gccgatgttc gacagataac cggtaatcag cggaataaca aagttagcca
     1681 gtccacccat catcatataa atactggtga ctttggcttt gcttttgggg aaaaactctg
     1741 acatgaccga aacaccgagc tgtaaaatgc cgccagctgc tgagaaaccg ataacaaagg
     1801 cgccggcatt acacaccagt ggagaagggt acaggtagat aatggcagct gttattgttg
     1861 ccagcgcaga gttaaataca ttagcccaga tgggccggac catttttttc agtagtgcgg
     1921 caaaaataaa gacacagacc aacgagccca tactgtaata agagatggtt tttaatgcct
     1981 cagcttctga cataccagca aaagccatcg catatttggg catccacacc acaatcacat
     2041 aaaaggtcga gaatgcggct acaccgaaca gtaccgatga aacaccttcc agccagacta
     2101 aaggttttgc tgttcatttg cggtaattca ttagctacgc tggcgtccac caactggctg
     2161 gggaatttgc ttttcaacag catcagcgtg atcagtacaa acagaatacc cggaataatc
     2221 agcccatagc cgtaccagat attattgagc aacatatagc tcaccagcat tgggtagaac
     2281 atttgcccaa atgacaccat cgctttaacc agtatgaccg ccgaaccaga ggctttcgga
     2341 aagcattcca tgagcgcggg gtagccaccc gtatccagcg ctgagttagc gatacctacg
     2401 cacactgcca gaccgtaggc gagagttaaa ttcgggcaag cgggaatacc aaagaagaat
     2461 agcagataca ttattactgc cattaatatc accgcccgac gaccaaactt atcggagatc
     2521 acaccgaaga ataaaatact gatcaatcgc cccaaaccga taccggaaat taaataggca
     2581 atgcccgcgt tgtcagtgga aaacttttct gacagagatg tcatattttg ggcaagcgta
     2641 ataacactaa tgccgtgcag gaaatagctg aagtaaatac aaagaacagc caggataaat
     2701 ggcgtgctga aagataattt cgttaccgta cgtattgttc tccgacggtt tacataatta
     2761 ctatactgaa gatgattttc cctgggtaca tggcataaag tttatgtttc caggaatatt
     2821 gcatgtcata gtttgttttt gatcgcgatt ttccttagtt agcgcagaaa gagttattta
     2881 tccgtaagat cttgcatgac ctgaaaagaa tattgcatta cccacccgga gcgacatttt
     2941 cctttagcga gtgatgccgc gtttttttgc ggctgcgtaa gccgataaac agtgccagta
     3001 aaaaaccgat ggcagtatcg aaccacataa tatcggcaat acttctttgg gacagatgag
     3061 ccgtaatcaa cggaatagta aaggtcgcaa tactgcccgt actgtaatag atccctgtag
     3121 ctttaccttt agcgtaaggg aaacgttcag ccattaacgt caggccaatt tgcacaacac
     3181 ctccagcaga ggtaaaacca atgacaaaag caaatattat caccacataa aatgtgggat
     3241 gcaggcagac ggtaaacaga gcaataaatg agataaaggt gtacagcatc agtaatgttg
     3301 ttgggcgaac ggtattacga atgagtggag cggtaataaa tacacaaagc agcgaaccca
     3361 cggtgtagat actgagtagt ttgatcgaca tagtgtatga catgcctgca acaaattgtc
     3421 cgtactgtgc cagccactgg ctaaccagat aaaacgttgc cattgagata tagccatata
     3481 aggtataact ggctaaatcg ataattgaac agcgatgttc cgtagagctg gtggttttct
     3541 ttatgacagg taagcgacga cccggatggg gtgggaacgt acaacggtat aaaaacagag
     3601 cgttaataaa cataatgcct gcagcaatca taaaggacca accgaaccac agttcagccc
     3661 acactaacag gctaatgatt agcggtaata aaaattgtcc gctggaaaca aatgctttaa
     3721 ttaaaatatt ggctgtccca ggtgagcgtg gaaaagcttc catcaaactg ggataagtgc
     3781 ctgcatcgag aaaactgttt gccattcccg ccagaaagcc aaaaacataa gcgataatga
     3841 tgttattggt ctgcaggatg tcaaaaaaga aggccatata gcagcacatc ccgagcatga
     3901 taaaagggcg gcgaccaaag cgatcggata ataatcctgc aaaaagcaag acacttaatc
     3961 gaccaatgcc cagcgatgag ataactatcg agacacccgc ggcattagtc tgccaaagtg
     4021 tctccagcga ggccatattc aggctcatca aaatgacgcc cataccatgc accaggtaat
     4081 taaaatacaa cccaagtgcg gtagggtaat agggattttt cataggttct acttagcaaa
     4141 ggtgctttaa aaagtgtagc gatgttgtgt cagtatccat tcattattgt tgtaacaacg
     4201 acaattcctt tctgtggaaa aattaacaaa gccgctccag atcgtgtgca tataattcag
     4261 cggctaccga ttgatagatc ttccagtcaa taaaattgcc atcagggtag acttgttgaa
     4321 acgcggttaa gtcggggaaa aaacgcatgg tgttgttgcc gatgcggtta tttattttct
     4381 ctatgatggc atgaagatgt ttttttcgtt gctgacgcat agccaacagt tgctcgacta
     4441 cacaatcagg aatggcgcat ttgccatttt cccactgacg ccagctttca ctgtcaccgg
     4501 tctgggcaat ccaggtcgcg cattcatcaa tagtcatggc aaaaatatgg cggagtgcct
     4561 ggagttcata agcgttcatc gtgtaatggc tgcatgaaat aaacaataaa atataaataa
     4621 cattatcagc ctgctgacgg cttgaagaga gtcacagtat cttgtgcaac attattggaa
     4681 agagtgaggg gcgaggtagc gaagcggaaa aatgttaaaa aaaagcccat cgtgggagat
     4741 gggaaaagac tacacacagc aattcgttgt ttcactcagg ggatttccat gcttataaat
     4801 caatatgttg atttataacc gtgagctaat agtaggcata cggacttttt gcgtcgatca
     4861 gattcgtctc attagtttaa aaactgtaaa gaatttgcga caggtactac cagctgaatg
     4921 gcggtaagca ggaaaatatg gggaagagca agcaaatatc aattagcggt gattaacaat
     4981 caggcggcag agccgcctga tgagaaatta cttattcggt ttctcgattt cgccaagttc
     5041 ttcgagaatt tcttccggtt ggtccgtcgg tggcgggact tcttccaccc acgcggcaaa
     5101 cagacgccag gaaacggcta acagaaccgg accaataaac agaccgatca tcccgaaagc
     5161 aatcaaacca ccaataacgc cagagaggat caggatcagc ggtaaatcgg cacccatgcg
     5221 aattaacatt gggcggatga cgttatccag cgtgccaacc acaccgctcc acactaacaa
     5281 taccgttccc caggtggtat cgccggtcca gtagagccag ataatcgccg gaatcagtac
     5341 cggcaacggg ccaagctgga caaggcagga gaggatcatt aacaccgtta gcaacgttgc
     5401 ataaggtacg ccggatacgg cgaggccgat accgccaagc actgcctgta ctaacgccgt
     5461 caccaccaca cccagcgcca ccgcgcggat agcctgtgcc gccagcagca cggcggcatc
     5521 tccgcgaacg cctgccagac gggttgcaaa atggcgaatg ccttgtgcca cctgttcacc
     5581 gcgccagtac agcagggcac tgaagagaag catcagcgca caatgcacca taaagcgccc
     5641 gatatgcgcc gcctgcccaa cgaaccaggt ggtggtggtg ccaatataag ggcggacttt
     5701 cgccatgatc gccgtacccc ccatatccag caagttgtgc cagcctgcat acagcttcgc
     5761 gccaatcacc ggaatggtat taagccacgc cagatcgggt aacgtcatgt cgccgctgga
     5821 aatggcttta attagcgggc cgctgccgtc gacgatactg ttaaccagca aagcaatagg
     5881 gatgataaac accatcacta ataacagcgt catcaccaga acggcgagag agcggcggcc
     5941 aaacatgatc ttttgcaaac gtaacaatac cggccaggtg gcgataacca ccgtaccggc
     6001 ccatgcaaag ccgagaataa agggttgaac aatccacaga catgccacaa tcatgatggc
     6061 taaaaacagc accgaaagca gaatttgtgc gacatccctg ggctgacgaa catttaccat
     6121 aactactttt cacctttttt gtgcgtcaaa tcgtcgacgc gacgtgaaca cgcgaaactc
     6181 acctgcataa tgatgagcaa tttcagcggt ttttaacagg ccgattctgc ccttaattct
     6241 gatgggcgtg ttagaaaaaa atgtggtaca acaactcagg caacacgcaa acgatttact
     6301 cgtcgtattt caagttgcat gtgctgcggc tacgttcgtt caccccagtc acttactatt
     6361 gtaagctcct ggagttcact cgcttgccgc cttcctgcaa ctcgaaatcc atagagtaaa
     6421 taaacatcac ctttcagtgc gctattgcag ccgcctgaaa gaatagtgta tcgacctaca
     6481 tcacagaccg caggaaaggg tcaatataat gattccacag atttccctgg cacccggcgt
     6541 cgttcaactg gtgcttaatt ttttgcaaga gctggagcaa caaggtttta ccggcgatgc
     6601 ggcgacaagt tatgccgatc gtctgacaat gtcgaccgac aacagtattt accaacttct
     6661 ccccgatgcg gtggtatttc cgcgttcaac cgcagatgtg gcgctgatcg cccgtcttgc
     6721 cgcgcaggaa cgctattcat cgctgatctt taccccccgc ggcggcggca ccggcactaa
     6781 cggtcaggcg ctcaaccagg ggattattgt tgatatgtcc cgccatatga accgcatcat
     6841 cgaaattaac cctgaagagg gctgggtgcg cgttgaggcc ggggtgataa aagaccaact
     6901 caatcagtac ctgaaaccgt tcggctactt ttttgcgccg gaactttcga ccagcaaccg
     6961 ggcaacgctc ggtgggatga tcaataccga tgcatccggt cagggatcgc tggtctatgg
     7021 caaaacgtca gatcacgtac ttggcgtacg cgcggtgttg ttggggggcg atattctcga
     7081 tacgcaacct ttacccgtcg agctggcgga aacgctgggt aaatccaata ccacaatcgg
     7141 gcgaatttat aacacggttt atcaacgttg ccgtcagcaa cgccagttaa tcatcgacaa
     7201 cttccccaaa cttaaccgct ttcttaccgg ttacgatctg cgtcatgtct ttaacgatga
     7261 gatgaccgag ttcgacctga cgcgcattct gacgggttca gaagggacgc tggcctttat
     7321 taccgaagcg cggctggata ttacgcgctt gcctaaagtg cgccgtctgg tgaatgtcaa
     7381 atatgactct tttgactctg cgctgcgtaa cgcgccgttt atggttgagg cgcgggcgct
     7441 ttcggtagag acggtggact caaaagtgct gaatctggcg cgggaagata ttgtctggca
     7501 ttccgtcagc gagttgatta ccgatatgcc tgacaaagag atgctcggac tgaacattgt
     7561 ggaatttgct ggtgatgatg aggcgctgat tgacgagcga gttaatgcac tctgtgtgcg
     7621 gcttgatgag ctgattgcca gccaacaggc aggtgtgatt ggctggcagg tgtgccgcga
     7681 gctggcgggg gttgaacgta tctatgcgat gcgcaaaaaa gccgttggtc tgcttggcaa
     7741 tgccaaaggt gccgctaagc caattccgtt tgctgaggat acctgcgtac cgccggaaca
     7801 tctggccgat tatattgctg aatttcgcgc gctgctcgac agccacggct taagctacgg
     7861 tatgttcggt cacgtcgacg caggtgtctt gcacgtccgt ccggcactgg atatgtgcga
     7921 tccgcaacaa gagattttga tgaagcaaat ctctgatgac gtggtggcgc tgactgcgaa
     7981 atacggtggt ttgttgtggg gcgagcacgg caaaggtttt cgcgctgaat acagcccggc
     8041 gtttttcggt gaggaacttt ttgcagaact gcgcaaagtg aaagcggcat ttgacccgca
     8101 taaccgactc aacccaggga agatttgccc gccagaaggt ctcgatgcgc cgatgatgaa
     8161 agtggacgcg gtgaagcgcg gtacattcga tcggcagatc cccattgcgg tacgccagca
     8221 gtggcgcggt gcgatggagt gtaacggcaa cggtttatgc ttcaactttg atgcccgtag
     8281 tccgatgtgt ccgtcgatga agatcaccca gaaccggatt cattcaccga aagggcgcgc
     8341 aacgctggtg cgtgaatggc tgcgcttgtt ggcggatcgc ggcgttgacc cactcaaact
     8401 ggaacaagaa ctgcctgaat ccggcgtcag tttgcggacg ttaattgccc gcacgcgcaa
     8461 tagctggcat gcgaataaag gcgaatatga cttctcacac gaagtcaaag aggcgatgtc
     8521 gggctgtctg gcctgtaaag cttgttcgac ccagtgcccc atcaaaattg atgtgccgga
     8581 gtttcgctct cgttttctgc agctctatca cacccgttat ttacgcccgc ttcgcgacca
     8641 cctcgttgct acggtcgaga gctacgcacc gctgatggca cgcgcgccga aaacctttaa
     8701 cttcttcatt aaccagccgc tggtgcgcaa actctcggaa aaacatatcg gcatggttga
     8761 tttgccactg ctgtcggtcc cctcgctaca acaacaaatg gtggggcatc gctcggcaaa
     8821 catgacgctg gaacagcttg aagctctcaa tgcagagcag aaagcgcgca cggtgttggt
     8881 ggtgcaggac ccctttacca gctattacga tgcgcaagtg gtggcggatt ttgtccgtct
     8941 ggttgaaaaa ttaggtttcc agcctgtgtt actgccattt tcgccaaatg gcaaagccca
     9001 gcatatcaaa ggctttctta atcgttttgc gaagacggcg aaaaagacgg cggatttcct
     9061 caaccgtatg gctaagcttg ggatgccaat ggtgggcgtc gatccggcgc tggtactttg
     9121 ttatcgcgat gaatataaac tggcgctggg cgaggaacgt ggcgagttta acgtcttact
     9181 tgcgaatgaa tggctggcaa gcgcacttga gtcacatcca gtggctacag tcagcggtga
     9241 atcatggtat ttctttggtc actgtaccga ggttaccgct ttgccgggcg cgccagcaca
     9301 atgggccgcg atatttgccc gttttggcgc gaaactggaa aatgtcagcg tgggttgctg
     9361 cggcatggca gggacttacg gacatgaagc gaaaaaccat gaaaattcgc tcgggatcta
     9421 tgagttatcc tggcatcagg ctatgcagcg actgccgcgt aaccgctgtc tggcgaccgg
     9481 atattcctgc cgtagccagg taaaacgggt tgaaggcacg ggggtacgcc atcctgtgca
     9541 ggctttactg gagattatta aatgatatgg aaacggaaaa tcaccctgga agcactgaat
     9601 gctatgggtg aaggaaacat ggtggggttc ctggatattc gctttgaaca tattggtgat
     9661 gacacccttg aagcgacaat gccagtagac tcgcggacaa agcagccttt cgggttgctg
     9721 catggaggag catccgtggt actggccgaa agtatcggtt ccgttgccgg ttatttatgt
     9781 accgaaggtg agcaaaaagt ggttggtctg gaaatcaatg ctaatcacgt ccgctcggca
     9841 cgagaagggc gggtgcgcgg catatgtaaa ccgttgcatc tcggttcgcg tcaccaggtc
     9901 tggcagattg aaatcttcga tgagaaaggg cgtttgtgct gttcgtcacg attgacgacc
     9961 gccattttgt gacgttgtca ccagaaaagt gtgacgcata ttgcttttgg tcattatctg
    10021 cacagtaaac ggtggtatac tgtgcaggtc ttgtctaaaa gcgaggatgc tatgtctact
    10081 caacttgatc ccacccagct ggccattgaa tttttacgcc gtgaccagag caatctttca
    10141 cctgcccaat atctcaaacg tctgaaacaa ctagaacttg aatttgctga cctgcttact
    10201 ctctcttctg ctgaattaaa agaagaaatc tacttcgcct ggcgtctggg cgtgcattga
    10261 tcctgactta atgccgcaag ttcgccaaat cgacctggat taacctccgt ccaggttcga
    10321 tgaccttctt aatactgctt atctaccgcc tgatattgtt caaaaataat actaatcgca
    10381 gtttgtgtta aaacggcggg ttagctttat gagattattc ttatcgcccc ttcaagagct
    10441 aagccactga gagtgccgga gataagcgcc ggatggggta gaaaccctta agcctgtgtc
    10501 gcacagactt aagggttttc ttatttctga acgctgcact tttttaccta tctcttaaat
    10561 agtcattttc aatacaactt tttctttttc atcacagcct taatgctcct tagataataa
    10621 ccattatcta acaatgagat acctaattct tagcgtgcct gttaacccac acatcagggg
    10681 ctatgcttat taaaagcagg caaaaagaat gttttacggt aaagcccctg cgtttgctgg
    10741 gttgaactga taatcattat cactaacatg ctgttatacg ctgaaagcga tgaagtgagg
    10801 taaatagatg gacatgcatt caggaacctt taacccacag gatttcgcct ggcaaggctt
    10861 aacgctgaca cccgcagcgg cgatacacat ccgcgagctg gtggcaaagc agccaggtat
    10921 ggtcggcgtg cgcttaggtg tgaagcaaac gggctgcgcg ggctttggct atgtgctcga
    10981 cagtgttagc gagccggaca aagacgatct gctgtttgaa cacgacggcg cgaagctgtt
    11041 tgtcccgctg caatcgatgc cgtttattga tggcacggaa gtcgatttcg ttcgtgaagg
    11101 acttaatcag atattcaaat ttcacaaccc taaagcccag aatgaatgtg gctgtggcga
    11161 aagctttagg gtataggcgg tactatgtct cgtaatactg aagcaactga cgatgtcaaa
    11221 acctggaccg gcggcccgct gaactataaa gaaggattct tcacccagtt agccaccgat
    11281 gagctggcaa aggggataaa cgaagaggtg gtgcgcgcaa tttcggcgaa gcgtaatgag
    11341 ccggagtgga tgctggagtt tcgtctaaac gcctatcgcg catggctgga gatggaagaa
    11401 ccgcactggt tgaaagcgca ctacgacaag ctgaattatc aggattacag ctactactca
    11461 gcaccatcgt gcggtaattg tgacgacact tgcgcgtctg aacctggcgc ggtgcagcaa
    11521 actggcgcga acgccttttt aagtaaagag gtggaggcgg cgtttgagca gttgggcgtt
    11581 cccgtgcggg aaggcaaaga ggtggcggtg gatgccattt tcgactctgt ttcggttgcc
    11641 accacttatc gcgaaaaact ggcggagcag ggaattattt tctgttcctt tggtgaggcg
    11701 atccacgatc acccggaact ggtgcgtaaa tatctcggca ccgtggtgcc ggggaatgac
    11761 aacttctttg cagcacttaa tgcggcggta gcctctgacg gtacgtttat ttatgtgcct
    11821 aaaggcgtgc gctgcccgat ggaactttcc acctattttc gcattaacgc ggaaaaaacc
    11881 gggcagtttg agcgcacaat tctggtggcc gacgaagaca gctacgtcag ctacattgaa
    11941 ggctgttccg ctccggtgcg tgacagctat cagttgcacg cggcagtggt ggaagtcatc
    12001 atccataaaa acgccgaggt gaaatattcc acggtacaaa actggttccc tggcgataac
    12061 aacaccggcg gtattctcaa cttcgtcacc aagcgtgctt tgtgcgaagg cgaaaacagc
    12121 aaaatgtcat ggacgcaatc agaaaccggg tcagcgatta cgtggaaata tcccagctgc
    12181 attttgcgcg gcgataactc cattggtgag ttttactcag tggcgctgac cagcggtcat
    12241 cagcaagcgg ataccggcac caagatgatc cacatcggta aaaacaccaa atcgaccatt
    12301 atctcgaaag ggatctctgc cggacatagt cagaacagtt atcgcggctt agtgaaaatc
    12361 atgccgacgg caaccaatgc gcgcaatttc actcagtgcg actcaatgct gattggcgct
    12421 aattgtgggg cgcatacctt cccgtatgtc gaatgtcgca ataacagcgc acaactggag
    12481 cacgaggcaa cgacatcacg tattggtgaa gatcaactgt tttactgcct gcaacgcggg
    12541 atcagcgaag aagacgccat ctcgatgatt gttaacggtt tctgcaaaga cgtgttctca
    12601 gaactgccgc tggaatttgc cgttgaagca caaaaactcc tcgccatcag tcttgaacac
    12661 agcgtcggat aaggaataaa catgttaagt attaaagatt tacacgtcag cgtggaagat
    12721 aaagctatcc tgcgtggatt aagcctcgac gttcgtcccg gcgaagttca cgccattatg
    12781 gggccaaacg gttcgggcaa aagtacctta tcggcaacgc ttgccgggcg cgaagattat
    12841 gaagtgacgg gcggcacggt tgagttcaaa ggcaaagatt tgcttgcact gtcgccggaa
    12901 gatcgtgcag gcgaaggcat ctttatggcc ttccagtatc cggtggagat tccaggcgtc
    12961 agtaaccagt ttttcctgca aacggcactt aatgcagtgc gcagctatcg cggccaggaa
    13021 acgctcgacc gctttgattt tcaggatttg atggaagaga aaatcgctct cctgaagatg
    13081 ccggaagatt tattaacccg ttcggtaaac gttggttttt ccggcggcga gaaaaagcgc
    13141 aacgatattt tgcaaatggc ggtgctggaa ccggagttat gcattcttga tgagtcggac
    13201 tccgggctgg atattgacgc attaaaagtg gtcgccgatg gcgtgaactc gctgcgtgat
    13261 ggcaagcgct cattcatcat tgttacgcac taccaacgca ttctcgacta catcaagcct
    13321 gattacgttc atgtgctata tcagggacga attgtgaaat ccggcgattt cacgttggtc
    13381 aaacaactgg aggagcaggg ttatggctgg cttaccgaac agcagtaatg cgctgcaaca
    13441 gtggcatcac ttgtttgaag ctgaagggac aaaacgctca ccgcaagcac agcagcattt
    13501 acaacaattg ctgcgtaccg gactgccgac acgtaaacat gaaaactgga aatatacgcc
    13561 gctggaaggg ctgaccaata gtcagtttgt cagcattgcg ggagatatat ccccacagca
    13621 gcgtgatgcc ttagcgttaa cgttagacgc cgtgcggctg gtgtttgtcg atggacgtta
    13681 cgtgcccgcg ctgagcgatg caattgaagg cagcggatat gaagtgagca ttaacgacga
    13741 ccgtcagggg ttacccgacg ctattcaggc ggaagtgttt ctgcatttga cggaaagcct
    13801 ggcacaaagc gtgacgcata tcgccgtgaa gcgcggtcaa cggccggcaa agccattgct
    13861 gttaatgcat atcacccagg gcgtggcagg tgaagaggtg aacactgccc attaccggca
    13921 tcatctggat ctggcggaag gtgccgaagc aacggtgatc gaacattttg tcagccttaa
    13981 tgatgctcgt cattttaccg gggcacggtt cactatcaat gtcgcagcga acgcccactt
    14041 gcagcatatc aagctggcgt ttgaaaaccc ggtcagtcac cactttgccc ataacgattt
    14101 gttgctggct gacgatgcca ccgcatttag ccatagtttc ctgctgggtg gcgcagtgtt
    14161 acgacacaac accagtacgc aactcaatgg cgaaaacagc acgctgcgga tcaatagcct
    14221 ggcgatgccg gtgaaaaacg aggtgtgtga tacccgtacc tggctggaac acaataaagg
    14281 tttttgtaac agccgacagt tgcacaaaac tatcgtcagc gacaaaggcc gcgcggtatt
    14341 taacggtttg atcaacgtcg cgcagcacgc catcaaaacg gatggtcaga tgaccaacaa
    14401 taatctgctg atgggcaaac tggcggaagt ggatacaaaa ccgcagctgg aaatctatgc
    14461 agatgatgtg aaatgcagcc acggcgcgac ggtggggcgt attgatgatg aacagatgtt
    14521 ctatctgcgc tcgcgcggga tcaatcagca ggatgcccag cagatgatca tttacgcctt
    14581 tgctgccgaa ctgacggaag cactgcgtga tgaggggctt aaacagcagg tgctggcccg
    14641 aatcggtcaa cggctgccag gaggtgcaag atgacttttt ccgtcgacaa agtgcgggcc
    14701 gactttccgg tgctttcgcg tgaggtaaac ggtttgccgc tggcttatct cgacagcgcc
    14761 gccagtgcac agaaaccgag ccaggtgatt gacgccgagg ccgagtttta tcgtcatggc
    14821 tacgcggcgg tgcatcgcgg tattcatacc ttaagcgccc aggcgaccga gaaaatggag
    14881 aacgtgcgca agcgggcatc gctgtttatt aatgcccgtt cggcggaaga gctggtgttt
    14941 gtccgcggca cgacggaagg gatcaatctg gtcgccaata gctggggcaa cagcaatgtg
    15001 cgggcgggcg ataacatcat catcagtcag atggagcacc acgctaacat tgtcccctgg
    15061 cagatgcttt gcgcacgcgt tggcgcagag ctgcgtgtga tccctctcaa ccccgacggt
    15121 acgctgcaac tggagacgct gcctacgctg tttgatgaga aaactcgcct gctggcaatt
    15181 actcatgtct ccaacgtgct tggcacagaa aatccactgg cggaaatgat cacgcttgcg
    15241 caccagcatg gcgcaaaagt gctggtggat ggcgctcagg cggtgatgca tcatccggtg
    15301 gatgttcagg cgctggattg cgacttttac gtgttctccg ggcataaact gtatggcccc
    15361 accggaattg gcattcttta tgtgaaagaa gccttgttgc aggagatgcc gccgtgggaa
    15421 gggggcggtt ctatgatcgc caccgtcagc ctgagtgaag gcactacctg gaccaaagca
    15481 ccatggcggt ttgaagccgg tacacccaat accgggggca tcattggtct tggcgcggcg
    15541 ctggagtatg tttcggcgct ggggcttaat agcatagccg agtatgaaca gaatctgatg
    15601 cattatgcgc tatcacagct ggaatctgta ccggatctca ctctctatgg ccaacaaaac
    15661 aggcttggcg ttattgcttt taatctcggt aaacaccacg cctatgatgt tggcagtttt
    15721 ctcgataatt acggcattgc tgtgcgtacc ggacatcact gcgctatgcc attaatggcc
    15781 tattacaacg tccctgcgat gtgtcgggcg tcgctggcca tgtataacac ccatgaagaa
    15841 gtggagcgtc tggtgaccgg cctgcaacgt attcaccgtc tgctgggata acagggaggc
    15901 actatggctt tattgccgga taaagaaaaa ttgctgcgta attttttacg ctgcgccaac
    15961 tgggaagaga aatatctcta cattattgag ctgggccagc gtctgccaga attacgcgcc
    16021 gaagacggaa gcccacaaaa tagcattcag ggctgccaga gtcaggtgtg gattgtcatg
    16081 cgccagaatg cgcagggaat tattgaattg cagggcgaca gcgatgcggc gattgtgaaa
    16141 gggcttattg cggtcgtctt tattctctac gatcagatga cgccgcagga tattgtcaat
    16201 ttcgatgtgc gtccgtggtt tgaaaaaatg gcgctcaccc aacatctcac cccatctcgt
    16261 tcacaaggtc tggaagcgat gattcgcgca attcgcgcca aagccgctgc acttagctaa
    16321 actaaaggga cagctttcat ccggttggct cacattgccg gatggtcaat gtttacactg
    16381 accggcacag gagtgttttg gtttcaggtg aacataagca ccatccgacg tttcagcgtg
    16441 agtctccggc aaatacagga ggtttacaat gaaacgcgcg tctttgctta cactcacgct
    16501 tatcggcgct tttagcgcca tccaggctgc ctgggcggtt gattatccgc taccaccaac
    16561 cggaagccga ctggttgggc aaaatcaaac gtatacggtg caagaagggg ataaaaacct
    16621 tcaggctatt gcccgacgtt ttgatactgc agcaatgttg atccttgaag ccaataacac
    16681 tatcgccccg gtgccaaaac ctggtacgac gataactatt ccttcacaac tgttattacc
    16741 tgatgcaccg cgtcagggga ttatcgttaa ccttgcagag ctgcgccttt attattatcc
    16801 gccgggagaa aatattgtgc aggtttatcc aataggtatt ggattgcagg ggctggaaac
    16861 gccggtgatg gaaacgcgtg ttgggcagaa aatccctaac ccaacctgga cgcctacggc
    16921 aggcattcgt cagcgttcgc tggagcgtgg cattaaatta ccgccagtcg ttcctgccgg
    16981 accaaataac ccgctaggac gttacgcact gcgcctcgcg catggtaatg gcgaatacct
    17041 cattcatggc accagtgcgc cggacagcgt tggtttgcgc gtcagttcag ggtgtattcg
    17101 catgaatgca ccggatatta aagccttgtt ctccagcgtg cggacgggaa cgccggtgaa
    17161 agtgatcaac gaaccggtga aatattccgt ggaacctaac gggatgcgtt atgttgaagt
    17221 acatcgacca ctatcagcag aagaacagca aaacgttcag acaatgccat acacactgcc
    17281 agcaggcttt acgcaattta aagacaataa ggctgtagat cagaagttag tcgataaagc
    17341 gttgtatcgt cgggcagggt atccggtttc ggtgagcagt ggagcaactc ccgcagccag
    17401 caatgcgcct tcagtagagt cagcgcagaa tggtgaacca gagcaaggga atatgttacg
    17461 cgcgacgcag tagtggtaaa tagcagacaa aaaaatggcg cacaatgtgc gccatttttc
    17521 acttcacagg tactattact tgcggtattt agtagccatg ttgtccagac gctggttagc
    17581 acgagctgcg tcatctttag cagcctgaac gtcggaacgc attgcgttca cgtcgttgct
    17641 cagctggtca actttagcgt tcagagtctg aacgtcagaa gacagctgat cgattttagc
    17701 gttgctggag caacctgcca gcagagtaga acccaggatt accgcgccca gtaccagttt
    17761 agtagctttc attattaata ccctctagat tgagttaatc tccatgtagc gttacaagta
    17821 ttacacaaag ttttttatgt tgagaatatt tttttgatgg gaaggcactt atttttgatc
    17881 gttcgctcaa agaagcatcg aacgctgggt tttagataaa aaaattgaga gaaaacagga
    17941 tgcttccatc ggattcatct taaataaagt gagattatat agtgaaatcc gatttgattt
    18001 tttattgctt ctggttatcg attaaataaa aaaagcgccc atcagggcgc ttcgatatac
    18061 aaattaattc acaaaagcaa tattacagga cgtgaacaga tgcggtgtta gtagtgccgc
    18121 tcggtaccag tgcaccagaa accataacta cgacgtcacc tttgtgtgcc agaccgctct
    18181 gcagagccag ttctttaccc agacggtaga aatcatcagt agaagtgatc tctttaacca
    18241 gctgcggcac aacgcctttg ctcagtacca actgatgagc cgttttttcg ttggtggtca
    18301 gtgccaggat ggtggcatcc gggaagtatt tacgtactgc gcgagcagat ttaccgccct
    18361 gggtagcaac cacgatcagc ggagcatcca gtttttcagc agtttcaacg gcaccacggc
    18421 ataccgcttc ggtgatgcgc agtttacggt tgtcattgtt gaactcgaga cggctgttca
    18481 tcacgcggtc ggtacgttcg cagatggtcg ccatgataga aaccgcttcc agcgggtatt
    18541 taccttttgc ggattcacca gacagcatca ctgcgtcagt accgtcgagg atggcgtttg
    18601 caacgtcacc ggcttctgcg cgagtcgggc gtgggttttt gatcatggaa tccagcatct
    18661 gggtcgcagt gataacgact ttacgtgcac ggatacattt ttcgatcatc atcttctggg
    18721 cgaagataac ttcttctacc gggatttcta cacccaggtc gccacgcgca accatgatgc
    18781 cgtcagaggc ttcgaggatt tcgtcgaagt tgttgaggcc ttcctggttt tcgattttgg
    18841 agatgatgtg gatgttttcg ccgccgtgcg ctttcaggtg ctcacggatt tcgataacgt
    18901 cagaacgctt acgaataaag gaagcagcaa caaagtctac gcctttttcg caaccaaaga
    18961 tcaggtcctg tttgtctttt tcagccagtg ctggcagagc aatggaaacg ccaggcaggt
    19021 tcacaccttt gttttcgccc aggtcaccgt tgttcagcac tttacagata actttgttac
    19081 cttcaatggc ggtaacttcc ataccgatca gaccatcgtc aaccagtacg gtgttgccaa
    19141 cagacaggtc agtagtgaaa ccttcatacg ttaccgcaac catttcgctg ttgccgataa
    19201 cagatttatc agtggtgaaa gtaaaggtct gaccagcttt cagagaaaca tcgttaccgc
    19261 cttccagttt catggtgcgg atttccggac ctttggtatc aagcaggata gcggcggttt
    19321 taccagtttt gctcatcacg ttgcgcagat tctgaatgcg ctgaccgtgt tctgcatagt
    19381 caccatgaga gaagttcaga cgcataacgt tcatgccagc gtccagcatt ttagctaaca
    19441 tctcttcaga ttcggttttc ggtccgatgg tgcaaacaat tttggtc
//
LOCUS       DAJTQQ010000075        19293 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000075 DAJTQQ010000000
VERSION     DAJTQQ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19293
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.075"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..2095
                     /gene="pbpC"
                     /locus_tag="OO309_002847"
     CDS             <1..2095
                     /gene="pbpC"
                     /locus_tag="OO309_002847"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCR5847927.1"
                     /translation="ARYLEALINYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGS
                     TLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILYLNRAPFGGTLQGIGA
                     ASWAYLGKSPANLSYSEAAMLAVLPQVPSRLRPDRWPERAEAARNKVLERMAAQGVWS
                     REQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNW
                     KGRLPPRSSLAMIVVNHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVY
                     GLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPINMSEALVRSLNLPAVQVLEA
                     YGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLR
                     LQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPLAWKTGTSYGYRDAWA
                     IGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLSRSANLPEDPRPNSVT
                     RGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEGINGIRFPIWLDDKGQ
                     RVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGV
                     RDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLV
                     MDDVGQIATVKFVMQ"
     gene            2245..2676
                     /gene="ndk"
                     /locus_tag="OO309_002848"
     CDS             2245..2676
                     /gene="ndk"
                     /locus_tag="OO309_002848"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR5847928.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2826..3980
                     /gene="trmG/rlmN"
                     /locus_tag="OO309_002849"
     CDS             2826..3980
                     /gene="trmG/rlmN"
                     /locus_tag="OO309_002849"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR5847929.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4265..5278
                     /gene="rodZ"
                     /locus_tag="OO309_002850"
     CDS             4265..5278
                     /gene="rodZ"
                     /locus_tag="OO309_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR5847930.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5305..6432
                     /gene="ispG"
                     /locus_tag="OO309_002851"
                     /gene_synonym="gcpE"
     CDS             5305..6432
                     /gene="ispG"
                     /locus_tag="OO309_002851"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR5847931.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6535..7809
                     /gene="hisS"
                     /locus_tag="OO309_002852"
     CDS             6535..7809
                     /gene="hisS"
                     /locus_tag="OO309_002852"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR5847932.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7827..8447
                     /gene="yfgM"
                     /locus_tag="OO309_002853"
     CDS             7827..8447
                     /gene="yfgM"
                     /locus_tag="OO309_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR5847933.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8458..9636
                     /gene="bamB"
                     /locus_tag="OO309_002854"
     CDS             8458..9636
                     /gene="bamB"
                     /locus_tag="OO309_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR5847934.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9754..11226
                     /gene="der"
                     /locus_tag="OO309_002855"
     CDS             9754..11226
                     /gene="der"
                     /locus_tag="OO309_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR5847935.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11296..11511
                     /locus_tag="OO309_002856"
     CDS             11296..11511
                     /locus_tag="OO309_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR5847936.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="OO309_002857"
     CDS             complement(11508..12878)
                     /gene="xseA"
                     /locus_tag="OO309_002857"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR5847937.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13040..14506
                     /gene="guaB"
                     /locus_tag="OO309_002858"
     CDS             13040..14506
                     /gene="guaB"
                     /locus_tag="OO309_002858"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR5847938.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14575..16152
                     /gene="guaA"
                     /locus_tag="OO309_002859"
     CDS             14575..16152
                     /gene="guaA"
                     /locus_tag="OO309_002859"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR5847939.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16247..16786)
                     /locus_tag="OO309_002860"
     CDS             complement(16247..16786)
                     /locus_tag="OO309_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR5847940.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16802..17320)
                     /locus_tag="OO309_002861"
     CDS             complement(16802..17320)
                     /locus_tag="OO309_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5847941.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17631..17822)
                     /locus_tag="OO309_002862"
     CDS             complement(17631..17822)
                     /locus_tag="OO309_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR5847942.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17840..17992
                     /locus_tag="OO309_002863"
     CDS             17840..17992
                     /locus_tag="OO309_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5847943.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            18174..>19293
                     /gene="pdeF"
                     /locus_tag="OO309_002864"
     CDS             18174..>19293
                     /gene="pdeF"
                     /locus_tag="OO309_002864"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5847944.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGI"
BASE COUNT         4542 a         4698 c         5246 g         4807 t
ORIGIN      
        1 ggcacgttac cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg
       61 ggttaatcca ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat
      121 ttccggtggc agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac
      181 atttggtggc aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa
      241 gcgtgaaatt ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat
      301 cggtgcggca agttgggctt atctcggaaa atcgcctgcg aatttaagct attccgaggc
      361 ggcaatgctg gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga
      421 gcgtgccgaa gccgcgcgta ataaagtgct cgaacggatg gctgcgcaag gtgtgtggtc
      481 ccgtgagcag gtaaaagagt caagggaaga acccatctgg ctggcacccc gacaaatgcc
      541 gcaactggca ccgctgtttt cgcgcatgat gctcggtaaa agcaaaagcg acaaaatcgt
      601 tactacgctg gatgccggtc ttcaacgacg tctggaagaa ctggcgcaaa actggaaagg
      661 gcggttgcca ccgcgcagct cactggcgat gatcgtggtt aatcataccg atatgcgtgt
      721 tcgcggctgg gtgggatcgg ttgatctcaa cgatgattca cgctttggtc atgttgatat
      781 ggtcaatgcg atccgatcgc caggatcggt gctcaaaccg tttgtttatg gtctggcgct
      841 ggatgaaggc ttgatccacc cggcatcact gctgcaagac gtcccccggc gcaccggtga
      901 ttatcgacca ggtaactttg atagcggttt tcatggcccg atcaacatga gcgaggcgct
      961 ggtgcgctcg ctgaacttac ctgctgtgca ggtgctggaa gcctatggac cgaaacggtt
     1021 tgcggcaaag ttacgcaatg ttggattgcc gttatatttg cccaacggcg ctgcgccgaa
     1081 tctttcactc attctcggcg gcgctggtgc aaaactggaa gatatggcgg cagcgtatac
     1141 cgcgtttgct cgccacggca aggcaggcaa attgcgctta cagcctgacg atccgctgct
     1201 tgaacggcct ttaatgtcgt cgggggcggc gtggatcatt cgccggatta tggctgatga
     1261 agcgcaaccc ttgccggata gtgccttgcc gcgcgtcgcc ccactggcat ggaaaacggg
     1321 caccagctat ggctatcgtg acgcctgggc gattggggtt aacgctcgct atgtcattgg
     1381 gatctggact ggcagacctg acggcacgcc cgttgttggt cagtttggct ttgccagtgc
     1441 cgtaccattg ttgaatcagg tcaataatat cttactgtcg cgcagtgcga atctgccgga
     1501 agacccgcgc ccgaactcag tcactcgtgg cgttatctgc tggccgggcg gacagtcttt
     1561 gccggaaggt gacggtaact gtcgccgccg cctggcaacc tggctgctgg atggcagcca
     1621 gccgccaacg ctgttactgt cagagcagga agggattaat ggcattcgct tccccatctg
     1681 gctggatgac aaagggcagc gcgtcgccgc tgactgcccg caggcgaagc aagaaatggt
     1741 aaatgtctgg ccgctaccgc tggaaccctg gctgcccgca tcagaacgtc gcgctgtgcg
     1801 cttaccaccg gcctcgacaa tttgcccgcc atacggtcac gatgcccagc tccctctgca
     1861 gctgacaggc gttcgcgatg gcgcgattat taaacgttta ccgggcgcag cggaagcgac
     1921 tttgccgttg caatcaagtg gaggggcagg tgaacgctgg tggtttctga atggcgaacc
     1981 gttaactgaa cgcgggcgca acgtgaccct gcatttgacg gataaaggtg attatcaatt
     2041 gctggtgatg gatgatgtgg ggcaaatcgc gacagtgaaa tttgtcatgc aatagtctgt
     2101 tttgtccctg attgttgcta aaactcattt tattttaaaa aaaatgttac cttcatcaat
     2161 agtcaacggc cctgttgctc attataatcc gcgccatctc gtacgctggt acagacaaca
     2221 acagaacaat ttacagaggt aaaaatggct attgaacgta ctttttccat catcaaaccg
     2281 aacgcggtag caaaaaacgt cattggtaat atctttgcgc gctttgaagc tgcagggttc
     2341 aaaattgtcg gcaccaaaat gctgcatctg accgttgaac aggcacgtgg cttttatgct
     2401 gaacacgatg gaaaaccgtt ctttgatggt ctggttgaat tcatgacctc tggcccgatc
     2461 gtggtttccg tgctggaagg tgaaaacgcc gttcagcgtc accgcgatct gctgggcgcg
     2521 accaatccgg caaacgcact ggctggtact ctgcgcgctg attacgctga cagcctgacc
     2581 gaaaacggta cccacggttc tgattccgtc gaatctgccg ctcgcgaaat cgcttatttc
     2641 ttcggcgaag gcgaagtgtg cccgcgcacc cgttaataat ttcgtaaatg ccgcgtgcaa
     2701 acgtggcatc cgtgcgccag aatttgtaca atgcagcgcc cccggacgag cagccgctca
     2761 ccggggcgtt tcttttttca accctccagg ggccataacg tgtaataacg aggccggaat
     2821 agcatatgtc tgaacaatta gtcacacctg aaaacgtcac cacgaaagat ggaaaaatca
     2881 acctgctgga tctcaaccgt cagcagatgc gggagttttt taaagattta ggtgaaaaaa
     2941 ccttccgcgc cgatcaggtg atgaagtgga tgtatcacta ttgctgcgac aactttgatg
     3001 agatgaccga catcaacaaa gtgttgcgcg gcaaactgaa agaggtggcg gaaatccgcg
     3061 caccggaagt ggttgaagaa cagcgttcat ccgacggcac cattaaatgg gcgatcgccg
     3121 ttggcgatca gcgcgtcgaa acggtgtata tcccggaaga cgaccgtgcc acgctctgcg
     3181 tctcttcgca ggtggggtgt gcgctggagt gtaaattctg ttccaccgcc cagcagggct
     3241 ttaaccgcaa cctgcgggta tcggaaatta ttggccaggt gtggcgtgcg gcgaaaatcg
     3301 tcggcgcagc aaaagtcacc ggtcagcgcc caatcactaa cgtcgtgatg atgggcatgg
     3361 gcgagccgct gctcaacctg aacaacgtcg ttccggcgat ggaaatcatg cttgatgatt
     3421 tcggttttgg cctgtctaaa cgtcgcgtga cgctttccac ttccggtgta gttccggcgc
     3481 tggataaact gggcgatatg atcgacgttg cactggcaat ttccctgcac gcgccgaacg
     3541 acgaaattcg tgacgaaatt gtgccgatca acaaaaagta caatatcgaa acgttccttg
     3601 ctgcggtgcg ccgttatctg gagaaatcca acgccaatca gggccgagtc actattgaat
     3661 acgtgatgct tgaccacgtt aacgacggca ctgaacacgc gcaccaactg gcggaactgc
     3721 tgaaagatac gccgtgtaag atcaacctga tcccgtggaa cccgttcccg gacgcgccgt
     3781 atggacgcag ttcgaacagc cgtatcgacc gtttctcaaa agtgctgatg agctacggtt
     3841 tcaccactat tgtgcgtaaa acgcgtggtg atgatatcga tgctgcctgt ggtcagttgg
     3901 cgggcgatgt tatcgaccgt acgaaacgta ctctgcgtaa acggatgcag ggtgaagcca
     3961 tcgatattaa agcggtctga tagccattac gccacggtta ctttatgtac cgtggcgtaa
     4021 tcttttatga atgaagtatt aagttgcaat tgtctggtca atattaacgg tgcgtttttg
     4081 ctgactttaa ggcagtatgt aacgatgcaa cagtaagtta gcctcagtga atgtgggctt
     4141 tgtcacgagc acacagacgg tcttatactg tatgataacg gttaacttaa cggatgtttc
     4201 gcggtgtggg tgagcatgat ggttcaccgg catctcaatt ctcatttaaa cgtacctgca
     4261 gcgaatgaat actgaagcca cgcacgacca aaatgaagca cttactaccg gcgctcgcct
     4321 gcgtaatgct cgcgaacaac taggacttag tcagcaggcc gttgccgagc gactttgcct
     4381 gaaggtttcc acggtacgcg acattgaaga agataaggca cccgccgatc ttgcttcaac
     4441 attcctgcgc ggatatatcc gctcttatgc gcgtctggta catattccag aagaagaact
     4501 gctgccaggg ctggaaaagc aggctccact tcgggctgca aaagttgcgc caatgcagag
     4561 tttttccctc ggtaaacgcc gcaaaaaacg cgacggctgg ctaatgacct tcacctggct
     4621 ggtgttgttt gtggttatcg gcctgagcgg tgcctggtgg tggcaagacc acaaagctca
     4681 gcaggaagag atcaccacta tggccgatca atcttcggcg gaactgagca gtaatagcga
     4741 gcaggggcag agtgttccgt taaatacgtc gacaactaca gacccggcta caacgtccac
     4801 gccgccagcg tctgtggata ctaccgcaac caacacgcaa acacctgccg taactgcgcc
     4861 agcaccagct gttgatccgc aacagaatgc ggttgtttcg ccttcgcagg caaatgttga
     4921 taccgccgcg accccggtac caacggcagc aacaacgcca gatggtgctg cgccgttgcc
     4981 aaccgatcag gctggcgtga ccacgccggc ggctgatccg aatgcgctgg tgatgaactt
     5041 tactgccgat tgctggctgg aggtcactga tgctaccggt aaaaaattgt ttagcgggat
     5101 gcagcgtaaa gacggtaatt tgaatttaac cggccaggca ccgtataaac tgaaaattgg
     5161 tgcgccagcc gcagtacaga tccagtatca agggaaacct gtcgatctga gtcgttttat
     5221 cagaactaac caggttgcgc gtctgaccct caatgccgaa caatcaccgg cgcagtaaca
     5281 gacgggtaac gcgggagatt tttcatgcat aaccaggctc caattcaacg tagaaaatca
     5341 acacgtattt acgttgggaa tgtgccgatt ggcgatggtg ctcccatcgc cgtacagtcc
     5401 atgaccaata cgcgtacgac agatgtcgaa gcaacggtca atcaaatcaa ggcgctggaa
     5461 cgcgttggcg ctgatatcgt ccgtgtctcc gtaccgacga tggacgcggc agaagcgttc
     5521 aaactcatca aacagcgggt taacgtgccg ctggtggctg acatccactt cgactatcgc
     5581 attgcgctga aagtagcgga atacggcgtc gattgtctgc gtattaaccc tggtaatatc
     5641 ggtaatgaag agcgtattcg catggtggtt gactgtgcgc gcgataaaaa cattccgatc
     5701 cgtattggcg ttaacgccgg atcgctggaa aaagatctgc aagaaaagta tggcgaaccg
     5761 acgccgcagg cgttgctgga atccgccatg cgtcatgttg atcatctcga tcgcctgaac
     5821 ttcgatcagt tcaaagtcag cgtgaaagcg tctgacgtct tcctcgctgt tgagtcttat
     5881 cgtttgctgg caaaacagat cgatcagccg ctgcatctgg ggatcaccga agccggtggt
     5941 gcacgcagcg gggcagtaaa atccgccatt ggtttaggtc tgctgctgtc ggaaggcatc
     6001 ggcgacacgc tgcgtgtatc gctggcggcc gatccggtcg aagagatcaa agtcggtttc
     6061 gatattttga aatcgctgcg tatccgttcg cgagggatta acttcatcgc ctgcccgacc
     6121 tgttcgcgtc aggaatttga tgttatcggt acggttaacg cgctggagca acgcctggaa
     6181 gatatcatca ctccgatgga cgtttcgatt atcggctgcg tggtgaatgg cccaggtgag
     6241 gcgctggttt ctacactcgg cgtcaccggc ggcaacaaga aaagcggcct ctatgaagat
     6301 ggcgtgcgca aagaccgtct ggacaacaac gatatgatcg accagctgga agcacgcatt
     6361 cgtgcgaaag ccagtcagct ggacgaagcg cgtcgaattg acgttcagca ggttggaaaa
     6421 ataataacgt gatgggaagc gcctcgcttc ccgtgtatga ttgaacccgc atggctcccg
     6481 aaacattgag ggaagcgttg agggttcatt tttatattca gaaagagaat aaacgtggca
     6541 aaaaacattc aagccattcg cggcatgaac gattacctgc ctggcgaaac ggccatctgg
     6601 cagcgcattg aaggcacact gaaaaacgtg ctcggcagct acggttacag tgaaatccgc
     6661 ttgccgattg tagagcagac cccgctattc aaacgtgcga ttggtgaagt caccgacgtg
     6721 gttgaaaaag agatgtacac ctttgaggat cgcaatggcg acagcctgac tctgcgccct
     6781 gaagggacgg cgggctgtgt acgcgccggc atcgagcatg gtcttctgta caatcaggaa
     6841 cagcgtctgt ggtatatcgg gccgatgttc cgtcacgagc gtccgcagaa agggcgttat
     6901 cgtcagttcc atcagttggg ctgcgaagtt ttcggtctgc aaggtccgga tatcgacgct
     6961 gaactgatta tgctcactgc ccgctggtgg cgtgcgctgg gtatcttcga acacgtaact
     7021 cttgagctga attctatcgg ttcgctggaa gcacgcgcca attaccgcga tgcgctggtg
     7081 gcattccttg agcagcataa agaaaagctg gacgaagact gcaaacgccg catgtacact
     7141 aacccgctgc gcgtgctgga ttcaaaaaat ccggaagtgc aggcgcttct caacgacgct
     7201 ccggcattag gtgattatct ggacgaggaa tctcgtgagc attttgccgg tctgtgcaaa
     7261 ctgctggaga gcgcggggat cgcttacacc gtaaaccagc gtctggtgcg tggtctggat
     7321 tactataacc gtaccgtttt cgagtgggtg actaacagtc tcggctccca gggcaccgtg
     7381 tgtgcaggcg gtcgttatga cggtcttgtg gaacaactgg gcggtcgtgc aacaccggct
     7441 gtcggttttg cgatgggcct cgaacgtctt gtattgttag tacaggccgt taatccggaa
     7501 tttaaagccg atcctgttgt cgatatatac ctggtggctt caggtgctga tacacaatct
     7561 gcggctatgg cattagctga gcgtctgcgt gatgaattac tgggcgtgaa attgatgacc
     7621 aaccacggcg gcggcaactt taagaaacag tttgcccgtg ctgataaatg gggtgcccgc
     7681 gttgctgtgg tgctgggtga gtctgaagtg gctaacggca cagcagtagt gaaggatttg
     7741 cgctctggtg agcaaacggc agttgcgcag gatagcgtag ccgcgcattt gcgcacgtta
     7801 ctgggttaag gaaggagaag gacagcgtgg aaatttacga gaacgaaaac gaccaggtag
     7861 aagcggttaa acgctttttt gctgaaaatg gcaaagcact ggctgttggg gggattttgg
     7921 gcgttggcgc actgactggc tggcgctact ggaacagcca tcaggttgat tctgcacgct
     7981 ccgcttctct tgcctatcaa aatgcggtta ccgcagtgag cgaaggcaaa ccggatagca
     8041 tcccggcggc ggaaaaattt gctgctgaaa ataaaaatac ttatggtgcg ctggcttctt
     8101 tggaacttgc gcagcaattt gttgacaaaa atgaactaga gaaagctgcc gcccagttac
     8161 aacaggggct ggcagacacg agcgatgaaa atctcaaagc cgtgataaat ctgcgtcttg
     8221 ctcgcgttca ggtacagctc aagcaggctg atgccgcgct gaaaaccctt gataccatca
     8281 aaggtgaagg gtgggctgcc attgttgccg acttgcgtgg tgaagcattg ctgagcaaag
     8341 gtgataagca aggtgcgcgt agtgcatggg aagcaggcgt gaaaagcgat gttactccgg
     8401 cactgagcga aatgatgcag atgaaaatta ataatttgtc catctgagag ggacccgatg
     8461 caattgcgta aattactgct gccaggactg ctttccgtta cccttttaag cggctgttcg
     8521 ctgtttaaca gcgaagaaga tgtggtaaag atgtccccat tgccaaccgt tgaaaaccag
     8581 tttacgccga ccacggcgtg gagcacttcc gttggtagcg gcattggcaa cttctattcc
     8641 aatcttcatc cggcactggc ggacaacgtt gtctatgcag cggaccgcgc tggtttggta
     8701 aaagcgctga atgcggatga tggcaaagaa atctggtctg tcagcctggc cgagaaagat
     8761 ggctggttct ctaaagagcc tgcattactt tctggcggtg tgaccgtgtc tggtgggcat
     8821 gtctacattg gcagcgaaaa ggcgcaggtt tacgcgctga ataccagcga tggtactgtg
     8881 gcatggcaaa ctaaagtcgc gggtgaagca ctttcgcgcc cggtggtcag cgacggtctg
     8941 gtgttaatcc acaccagtaa cggtcagtta caagcgctga acgaagctga cggcgctgtc
     9001 aaatggacag ttaacctcga tatgccttcg ctctctttgc gtggcgagtc tgcgccggca
     9061 acggcttttg gtgcggccgt cgtgggtggc gataatggtc gcgtcagcgc agtgctgatg
     9121 gaacagggcc agatgatttg gcagcagcgt atttcccagg cgaccggttc taccgaaatt
     9181 gaccgtctga gcgatgttga cacgactccc gtcgttgtta acggcgttgt tttcgcgctg
     9241 gcctataatg gtaacctgac ggcgcttgat ctgcgcagtg gtcagattat gtggaaacgc
     9301 gaactgggtt cggtgaatga tttcatcgtc gatggcaatc gcatctatct ggtcgatcaa
     9361 aatgaccggg tgatggcgtt gaccattgat ggcggcgtta cgctgtgggc acaaagcgat
     9421 ctgctgcatc gcctgctgac ttctccggtg ctgtataatg gcaacctggt ggtcggtgac
     9481 agtgaaggtt atctgcactg gattaacgtc gaagatggtc gtttcgttgc ccagcaaaaa
     9541 gttgatagtt ccggtttcca gactgaaccg gttgccgctg acggcaaact gctgatccag
     9601 gcaaaagacg gaaccgtgta ctctattaca cgttaatcgt ctctgtcgtt cactttgaaa
     9661 acggctcctg gacaggggcc gttttcctgt ttttaacaac gacgcgaata tagcgtgcgt
     9721 tgtctgatga tttataaaaa tgaggcttta aacatggtac ctgtggtcgc gcttgtcggg
     9781 cgccctaacg taggaaaatc cacgttattt aaccgtctaa ctcgcacccg agatgcgctg
     9841 gttgcggatt tcccgggtct gactcgtgac cgtaagtacg gtcgtgcgga aattgaaggc
     9901 cgtgagttta tctgtattga taccggcggg attgatggca cagaagacgg tgtagaaacc
     9961 cgcatggcgg aacagtcgct gctggcgatt gaagaagcgg acgtcgtact gtttatggtg
    10021 gatgcgcgcg cgggcctgat gccggcagat gaagcgattg ccaaacatct gcgctcccgt
    10081 gaaaaaccga ccttcctggt ggtaaacaaa actgacggtc tggatcccga tcaggcagtg
    10141 gttgatttct actcgcttgg tttaggtgaa atctacccga tcgccgcgtc tcacggtcgt
    10201 ggcgtattaa gtctgctgga gcatgtgctg ctgccgtgga tggaagatct cgcaccgcaa
    10261 gaggaagtcg acgaagacgc tgaatactgg gcgcaatttg aagcggaaga gaacggcgaa
    10321 gaagaagagg aagacgactt cgacccgcaa agtctgccga tcaaactggc gattgtgggt
    10381 cgtccgaacg taggtaagtc tacactcact aaccgtattc ttggagaaga gcgcgttgtt
    10441 gtttacgaca tgcctggcac gacgcgtgac agcatctaca tcccaatgga acgcgatgga
    10501 cgtgagtatg tgctcattga caccgctggc gtacgtaaac gcggcaaaat caccgatgct
    10561 gtagagaaat tctccgtaat caaaacgttg caggccattg aagacgccaa cgtggtgatg
    10621 ttagtgattg atgcgcgcga aggtatttcc gatcaggatc tctcgctgct gggctttatt
    10681 ctcaatagtg ggcgctcact tgtcattgtg gtgaataagt gggatggcct gagccaggaa
    10741 gtgaaagagc aggtgaaaga aacgctggac ttccgtctgg gctttatcga ttttgctcgt
    10801 gtgcacttta tctctgcctt gcacggcagt ggtgttggta acttgtttga atcagtacgt
    10861 gaagcgtatg acagctccac ccgtcgtgtg gggacctcta tgctgacgcg catcatgacg
    10921 atggctgttg aagatcacca accgccgctg gtacgcggtc gtcgtgtgaa gctgaaatat
    10981 gcccacgctg gtggttataa cccgccgatt gtggtgattc acggtaatca ggtgaaagac
    11041 ctgcctgatt cctacaagcg ctacttgatg aactacttcc gcaaatcgct ggacgtaatg
    11101 ggatcgccga ttcgtattca gttcaaagaa ggggaaaacc cgtatgcgaa caagcgtaac
    11161 accctgacgc caacccagat gcgtaaacgt aagcgtctga tgaagcacat caagaaaaat
    11221 aaataattat ccgtaagtat tttatctgcc attccctcta tgaatgtaga gggaatggct
    11281 accggaggaa ttcatatgga actgcattgt ccaaaatgtc agcatgtgct tgatcaggat
    11341 aacggccatg cgcgttgccc atcctgcggg gaatttattg aaatgaaagc tctttgtcca
    11401 gactgccatc aaccgttgca ggtgttaaaa gcctgcggtg cagtagatta cttctgtcag
    11461 cacgggcatg gcttgatatc gaaaaaacgc gttgaattcg tgctggctta atgaaccttt
    11521 ttacgcgatt tttttaccgg ctggatgttt tttacttcac tttctatcca gccgtcttcc
    11581 agacgtgtgg ttagcatttc acccgtttta acttgcttca cttttttcag tacattgccg
    11641 tcagtagcag tagtaacgct gtatccacgc gccagcgttg acagtgggct tacggcttcg
    11701 aggtgcgtta ctgcattacc gaaacgttca cgcgtggcgc taagctgtgc gcgcaggatt
    11761 tctgctaaac gatattccag ttgctgaatg cgcgtttgcg tgcgatgaat cttcggctgt
    11821 ggattttgct gattcagccg ctgtgttaat cgctgctgct gttgcccggc acgcttaagc
    11881 tgattttcca gcgcaaagct catccgtttt tgcaggcgtt caagcatggt ttgctggcgt
    11941 gccagccgga gctgcggatg ctgctgctgc aagcgatgat gaatctgcgt aaagcgacgt
    12001 gtacggttgg cgagataata atccatcgcc atctccagcc gttgacgggc cgattgcacc
    12061 tggcgcagta actcttgctg attacggctc actacttcag cggcggcaga cggcgttggc
    12121 gcacgcagat cggcaacaaa atcggcaatg gtcacatccg tctcatgccc gacggcgctg
    12181 acgaccggaa tgcggctggc aaaaatcgcc cgcgctacgc gttcgtcgtt aaaactccat
    12241 aaatcttcca gcgaaccgcc gccgcgccca acgatcaaca cgtcgcactc attgcgctga
    12301 ttcgccagtt caatggcgcg aacaatttgc cccggcgcgt catcgccctg aacggaggtg
    12361 gggtagatga tcaccggtag agaaggatcg cgacgtttta acacatgcaa aatatcatgt
    12421 agcgcagcac cggtttttga ggtgatcaca ccaacgcaat gcgcagggga gggaagtggt
    12481 tttttgtatt gctgatcgaa caaacattca gcctgcaact tcgctttgag ctgttcgtac
    12541 ttctgttgca gcagcccttc accggccggc tgcatactct caacgattat ctggtagtcg
    12601 ccgcgcggct cgtagagcgt aatattggcg cgaactaaaa cttgttgccc atgctgcgga
    12661 cggaaggtga cccggcggtt gctgttgcgg aacatcgcgc agcgtacctg ggcggtgtcg
    12721 tctttgagtg taaagtacca gtgaccggaa gccggttgtg tgaaattaga gatttcgccg
    12781 ctgatccaaa cctgtcccat ctcatgctca agcagcagac gaaccgtttg attcaggcga
    12841 ctaacggtaa aaattgcagg ggattgagaa ggtaacatgt gagcgagatc aaattctaaa
    12901 tcagcaggtt attcagtcta tagtaacccg cccttcgggg atagcaagca ttttttgcaa
    12961 aaaggggtag atgcaatcgg ttacgctctg tataatgccg cggcaatatt tattaaccac
    13021 tctggtcgag atattgccca tgctacgtat cgctaaagaa gctctgacgt ttgacgacgt
    13081 tctcctcgtt cctgctcatt ctaccgttct gccgaatact gctgacctca gcacccagct
    13141 gacgaaaact attcgtctga atatccctat gctttccgca gcaatggata ccgtaacgga
    13201 agcgcgcctg gctattgctc tggctcagga aggcggtatc ggctttatcc acaaaaacat
    13261 gtccattgaa cgccaggcag aagaagttcg ccgtgtgaaa aaacacgaat ctggtgtggt
    13321 gactgatccg cagactgttc tgccaaccac gacgctgcgc gaagtgaaag aactgaccga
    13381 gcgtaacggt tttgcgggct atccggtcgt taccgaagaa aacgaactgg tgggtattat
    13441 caccggtcgt gacgtgcgtt ttgttaccga cctgaaccag ccggttagcg tttacatgac
    13501 gccgaaagag cgtctggtca ccgtgcgtga aggtgaagcc cgtgaagtgg tgctggcaaa
    13561 aatgcacgaa aaacgcgttg aaaaagcgct ggtggttgat gacgaattcc acctgatcgg
    13621 catgatcacc gtgaaagact tccagaaagc ggaacgtaaa ccgaacgctt gtaaagacga
    13681 gcaaggccgt ctgcgtgttg gtgctgcagt tggcgcaggt gcgggtaacg aagagcgtgt
    13741 tgacgcgctg gttgccgctg gcgttgacgt tctgctgatc gactcctccc acggtcactc
    13801 agaaggcgtt ttgcagcgta tccgtgaaac ccgtgctaaa tatccggatc tgcaaatcat
    13861 cggcggcaac gtggcaacag ctgcaggtgc acgcgctttg gcagaagctg gttgcagcgc
    13921 ggttaaagtc ggtatcggcc ctggttctat ctgtacgact cgtatcgtta ctggcgtagg
    13981 tgttccgcag atcactgccg ttgctgacgc agttgaagcc ctggaaggca ccggtattcc
    14041 ggttatcgca gatggtggta ttcgcttctc cggcgacatc gccaaagcta tcgccgctgg
    14101 cgcaagcgcg gtgatggtag gttccatgct ggcgggtact gaagaatctc cgggtgaaat
    14161 cgaactctac cagggccgtt cttataaatc ttaccgtggt atgggttccc tgggcgcgat
    14221 gtccaaaggt tcctctgacc gttatttcca gagcgataac gctgccgaca aactggtgcc
    14281 ggaaggtatc gaaggtcgcg tagcttataa aggtcgcctg aaagagatca ttcaccagca
    14341 gatgggcggc ctgcgctcct gtatgggtct gaccggctgt ggtactatcg acgaactgcg
    14401 tactaaagcg gagtttgtac gtatcagcgg tgcgggcatt caggaaagcc acgttcacga
    14461 cgtgaccatt actaaagagt ccccgaacta ccgtctgggc tcctgattct cttcgcccga
    14521 tttcatgtcg ggcgatttat attatctgtt tcacttgcct cggaataagc gtcaatgacg
    14581 gaaaacattc ataagcatcg catcctcatt ctggacttcg gttctcagta cactcaactg
    14641 gttgcgcgcc gcgtgcgtga gctgggtgtt tactgcgaac tgtgggcgtg ggatgtgaca
    14701 gaagcacaaa ttcgtgactt caatccaagc ggaattattc tttccggcgg cccggaaagt
    14761 actactgaag aaaacagtcc gcgtgcgccg cagtatgtct ttgaagcagg cgtaccgata
    14821 ttcggcgttt gctatggcat gcagaccatg gcaatgcagt tgggcggtca cgttgaagcc
    14881 tctaacgaac gtgaatttgg ctacgcgcag gtagaagtcg taaacgacag cgcactggtt
    14941 cgcggtatcg aagatgcgct gaccgcagac ggtaaaccgc tgctcgatgt ctggatgagc
    15001 cacggcgata aagttaccgc tatcccgtcc gacttcgtca ccgtagccag caccgaaagc
    15061 tgcccgtttg ccattatggc taacgaagaa aaacgcttct atggcgtaca gttccacccg
    15121 gaagtgactc acacccgcca gggtatgcgc atgctggagc gttttgtgcg tgatatctgc
    15181 cagtgtgaag ccctgtggac gccagcgaaa attatcgacg atgctgtagc tcgcatccgc
    15241 gagcaggtag gcgacgataa agtcatcctc ggcctctctg gtggtgtgga ttcctccgta
    15301 accgcaatgc tgctgcaccg tgctatcggt aaaaacctga cttgcgtatt cgtcgacaac
    15361 ggcctgctgc gtctcaacga agcagagcag gttctggata tgtttggcga tcactttggt
    15421 cttaacattg ttcacgtacc ggcagaagat cgcttcctgt cagcgctggc tggcgaaaac
    15481 gatccggaag caaaacgtaa aatcatcggg cgcgttttcg ttgaagtatt cgatgaagaa
    15541 gcgctgaaac tggaagacgt gaagtggctg gcgcagggca ccatctaccc tgacgttatc
    15601 gaatctgcgg cttctgcaac cggtaaagca cacgtcatca aatctcacca caacgtgggc
    15661 ggcctgccga aagagatgaa gatgggcctg gttgaaccgc tgaaagagct gttcaaagac
    15721 gaagtgcgta agattggtct ggagctgggc ctgccgtacg acatgctgta ccgtcatccg
    15781 ttcccgggac caggccttgg cgttcgtgtt ctgggtgaag tgaagaaaga gtactgtgac
    15841 ctgctgcgcc gtgctgacgc catcttcatt gaagaactgc gtaaagcgga cctgtacgac
    15901 aaagtcagcc aggcgttcac cgtcttcctg ccagtacgtt ccgttggcgt aatgggcgat
    15961 ggtcgtaagt atgactgggt tgtctctctg cgtgctgtcg aaaccatcga ctttatgacc
    16021 gcacactggg cgcatctgcc gtacgatttc ctcggtcgcg tttccaaccg cattatcaat
    16081 gaagtgaacg gtatttcccg cgtggtgtat gacatcagcg gcaagccgcc agctaccatt
    16141 gagtgggaat gattttgatc cctgcactat gaatgaacaa aaccctccgt tactacagag
    16201 ggttttttat cttcaagaat cataggcttg aagttactaa catcgattaa ttaaaccagc
    16261 tgtccgattt gttctcttct gctttgccca cgcttttcat cagatcgcga ccgccttcag
    16321 tcatatttct gtttgcgtca gcttcagatt gcaccacatc ggtttgcgca gctttgtgct
    16381 tcagttcctg atcgataaat tcgttttcgc gcttaacgcg ggcttcttct ttcgccagcg
    16441 ccagtttttg tttctgaatc tctaagctgc gtagctcatc ttcataactt tgatcgcgtt
    16501 ttttgtccgc agtggcttcg gcgtccagtt tatcctgacg agctttctta tttgccgctg
    16561 ccgttgccgc tcttttgttc gcggcggcct gggcatttgc gcgacgttgc ttctcttgct
    16621 ggatttccct gttgcgctcc gcgacccatt cgtcatgctg cctttgctct tcatttttac
    16681 cttgctgttc cgcttctgcg acagcagaga gttgatcctg caatgatgag gctatagccg
    16741 gatagcttaa ggaggctaag atggcgcaaa gaaaaacttt cttcatgact cctcctgatt
    16801 attagctctt ttcaggacat ttggtatttg gctgaatacg cgtttcgtta tacgttgtgg
    16861 taataacaac ggctaaacct gtcgtaaact ggcactcttt accctcctgg gtggaggtat
    16921 acactttggt gccttcctta tatgttaaag aaacaccttc cactaaggtt ttatcattca
    16981 ccatagaacc cgctgccgcg cctacagctc cgccgccaac tgcccctgcc gtcgttccgg
    17041 aattgctgcc agaaccgacg ttgtggccga taacaccgcc agcgactgcg ccaataagcg
    17101 cgccgaaggc ttgtgcgttc cgtttatttt gggagttgtc tacggcaact tttgcgggaa
    17161 gaatggaaat aatattaacg gttttagttt cttgtttggt attcagttga tcggtttgat
    17221 aaacatcggc ggcatgatcg tcagcatttg actggcatcc tgccagagtg aatgacgcta
    17281 acattgccac aggcagaaga cattttttaa atttcatcac tattccttgt tatacacatt
    17341 acgaaaaata agacatttaa gatcatcaaa agaggaaagt aaatgtattg cgtataaagg
    17401 gatagattaa atttattaaa attcacgcag ggaataattt ataaaaatat atcattacaa
    17461 catatatatt aattttatat ttatttcgca gccaaacagg ataaacatca gtcaggacaa
    17521 agacgtaaaa tttaagatga aagaatatct gccgctttga taatcccaaa gagcaaaagc
    17581 atccgacagc gaagtgccgt tatgttctgt gaaagtggag aaggtaaccg ctaacgttgt
    17641 accggtgatt cgacggagag tgtggattgc tgagcttctt ttttgctctg atggtgctgc
    17701 caggcaccga cggacgagta gataaaacgg ccaaagaaga agataaagct gatgagcagt
    17761 acgatacggg tcatgcgact gttaaatcgg tgtcgttttc gcatactagt tgcctgactc
    17821 acaaaaggtt ccttgaagta tgtcccacgc tgtggacggt acttacatta aggcacaaca
    17881 ggacaaaatg gtcaattctt cgttatgtaa aaaagcgtca gtggatacat attttaatgt
    17941 tatggaagtt aatttaatta tttacaatga tgatgtaata atgatgaatt aacatgataa
    18001 acgtaataat tcattaatca gggttatttt gtttgatata tatcaattgg aactttcata
    18061 tgacacttag aatcaatgct ctctatagtg atgaataatc atcattcgaa gtcaggtggg
    18121 atgcctgtct gaatacacct ccttcaggat gtgggggatt ctgctgagca tctatgaaac
    18181 tgaatgcaac ttatataaaa atacgtgata aatggtgggg gcttccgctg ttcctgcctt
    18241 ctttaatctt gcccattttc gcccacatta atactttcgc gcatatttct tccggtgagg
    18301 tttttctctt ttatctgcct ttggcactga tgatcagcat gatgatgttt ttcagctggg
    18361 cggcattgcc agggatcacc ttagggattt ttgtccgcaa atatgcagag ctgggttttt
    18421 acgaaacgct ctcattaacg gctaatttta ttatcattat cattctctgt tggggcggtt
    18481 acagggtctt tactccccgg cgtaacaacg tttcacatgg tgatacccgt ttaatttccc
    18541 agcgtatatt ctggcagata gtgtttcctg caacgctgtt tctgatactt ttccagtttg
    18601 ctgcatttgt aggattactg gcgagcagag aaaatctggt cggtgtcatg ccttttaacc
    18661 tcgggacttt aatcaattat caggccttac tggtgggtaa tctgatcggt gtcccgctgt
    18721 gctacttcat cattcgggta gtgcgaaatc cattttattt acgtagctat tattcgcaat
    18781 taaaacagca ggttgatgcc aaagtcacca aaaaagagtt cgcgctctgg ctactggcat
    18841 taggtgcttt attgttgctg ttatgcatgc cgttaaatga aaaaagcaca atttttagca
    18901 ccaattatac cttgtcatta ttgctgcccc tgatgatgtg gggagcaatg cgctatggtt
    18961 ataagctgat ttcgctgctc tgggcggtcg tgttgatgat cagcatccac agctatcaaa
    19021 attacattcc catttatcct ggctatacca cgcagctgac cataacctcc tccagttatc
    19081 tggtattctc ttttattgtc aattatatgg ctgtactggc aactcgtcag cgagcggtag
    19141 tcagacgcat tcagcggctt gcgtatgtgg acccggtggt tcatctgcca aatgttcgcg
    19201 ccctgaatcg cgcgttgcgt gatgccccct ggtctgcgct ttgttattta cgcatccctg
    19261 gcatggaaat gctggttaag aactatggca tca
//
LOCUS       DAJTQQ010000076        18804 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000076 DAJTQQ010000000
VERSION     DAJTQQ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18804
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.076"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(139..3654)
                     /locus_tag="OO309_002865"
     CDS             complement(139..3654)
                     /locus_tag="OO309_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5847945.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4107..5369)
                     /locus_tag="OO309_002866"
     CDS             complement(4107..5369)
                     /locus_tag="OO309_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5847946.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(5567..5642)
                     /locus_tag="OO309_002867"
     tRNA            complement(5567..5642)
                     /locus_tag="OO309_002867"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5607..5609),aa:Phe,seq:gaa)
     gene            complement(5748..6455)
                     /locus_tag="OO309_002868"
     CDS             complement(5748..6455)
                     /locus_tag="OO309_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR5847947.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            6853..8988
                     /gene="speC"
                     /locus_tag="OO309_002869"
     CDS             6853..8988
                     /gene="speC"
                     /locus_tag="OO309_002869"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR5847948.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OO309_002870"
     CDS             complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OO309_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR5847949.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OO309_002871"
     CDS             complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OO309_002871"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR5847950.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(11639..11914)
                     /locus_tag="OO309_002872"
     CDS             complement(11639..11914)
                     /locus_tag="OO309_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR5847951.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OO309_002873"
     CDS             complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OO309_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR5847952.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            13155..13874
                     /gene="trmB"
                     /locus_tag="OO309_002874"
     CDS             13155..13874
                     /gene="trmB"
                     /locus_tag="OO309_002874"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR5847953.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            13874..14200
                     /locus_tag="OO309_002875"
     CDS             13874..14200
                     /locus_tag="OO309_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR5847954.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            14384..15103
                     /locus_tag="OO309_002876"
     CDS             14384..15103
                     /locus_tag="OO309_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR5847955.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            15279..16325
                     /gene="ansB"
                     /locus_tag="OO309_002877"
     CDS             15279..16325
                     /gene="ansB"
                     /locus_tag="OO309_002877"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR5847956.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            16442..17404
                     /locus_tag="OO309_002878"
     CDS             16442..17404
                     /locus_tag="OO309_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR5847957.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(17505..>18804)
                     /locus_tag="OO309_002879"
     CDS             complement(17505..>18804)
                     /locus_tag="OO309_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR5847958.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILES
                     VVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIF
                     MDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGV
                     KIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         4899 a         4585 c         4644 g         4676 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 acttgtcaga aacatcaatc ttcgtcatca tttacaaaga aaaataatta gttaaagcag
     4081 tccgggctga ttgcccggac tgcttactat tttaatggat tgttaatctt tgcatattca
     4141 aacgggctga tacccttctc ccggttcgca tctagaaaat cggcccacca ttgcaacatc
     4201 agtcgccgtt cttccagatg ttctgcttta tggatatacg cagcacgtac accattacgc
     4261 tcctgatggc tcatctgacg ttccacagca tcgcgggacc acaaacctga ttcaatcaat
     4321 gcactgcacg ccatcgcccg gaatccatgg ccacagactt cctgggttgt gtcatacccc
     4381 atcaccctca gggctttatt tacagtattt tcactgattg gtttacgcgg gtcatgtgca
     4441 cccgtaaaga tcagaccatt ttcaccagcc catgtctgaa gttctgccag tatggctaca
     4501 acctgtttgc tgagtggcac aagatgtggt gttcgcatct tggatccccg gtgtgagaat
     4561 tttacgcctg gcagaggttc gcgttcggct ggaatagtcc acatggaatt gtcaatatca
     4621 atttccgacc agcgggcata tctgagttca ctggaacgaa taaaaaccag taaattcagc
     4681 ataactgcca gtcgggtgac aggctggcct ttataaccgt caatacgcga cagaagttca
     4741 ggcaggcgtg aaagctccag agcagggcga tgctggcgtt ttaccgtggt caatgcacca
     4801 gccatatcca gagccgggtt atatctgatg atgccagact gtacggcgta acgcattaca
     4861 gcagatattc tttgctgcag acgactggcg atttcataaa tttgttttgc ttctgcagca
     4921 cgaacaggga taagcaggtc cggggtattg agtgttgtga tatcccggtt gccgataaag
     4981 gggaaaacgt gtgtctcgag gcttttgagt actttatcgc tgtgtgattg tgcccattgt
     5041 ttattactgg catgccagcg tcgtgcaata tgttcaaaag agagctgtaa atcagggaca
     5101 acttttttaa ctcttttctg ttcgcaaggg tcaatcccct ctgaaatcag ttttcgggcc
     5161 tcatcccgtt ttgtacgagc ttcggagaga gaaacttctg gatacacacc aagtgccaga
     5221 gtcttctcct tacccagaat acgataacgg agacgccagt aacgggaacc attaggatga
     5281 accagcagga acatgccggc cccgtcagtg agtttataag ccttgtcagt gggctttgca
     5341 gcccggattt ttgcgtcagt cagtgccata gttcagcctt ctgaatgcgt taaagagggt
     5401 acaaattctg tcgaactggg gtatacccgc aattataccc acacaatggg gttgatgtgg
     5461 ggggaatctg gttgagttcg gtagaattga tttggaggaa gaacacttaa atcatggtgt
     5521 tctgaaacga aaaacggacc tccgtggagg tccgttgata tgaatttggt gcccggactc
     5581 ggaatcgaac caaggacacg gggattttca atcccctgct ctaccgactg agctatccgg
     5641 gcaacggggc gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac
     5701 tgcagactgc acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag
     5761 gcggtccagg cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc
     5821 gggaacattt taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg
     5881 gcgctgaagt ctgccatcat cgacggtgtg atcagcggta atatcagcgc ggcagcccac
     5941 gccagcgtta actggatgat cagtaatggg atactaatta ccgacaccgc aatacccagc
     6001 gagcaggcga agatcatcgc cgtaaagaaa tcaagaaatg acttggcgat taaaatactc
     6061 ggatcgccgg tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac
     6121 aggacaataa tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag
     6181 tgacgaaaca gattttgtgc tttggcgact gctgtattga cacctttctc cagcaaacaa
     6241 atttcgccaa ttagagcgcc aagtagcgta gctaaaacca tcgctggaag gttggcgcat
     6301 ttcacaacca gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag
     6361 acgcggatac gttccggtaa gcgttggctg agcagtgcgc cgagaacgcc acccagtaag
     6421 actgcactag cgttgataaa agggccgatg accacaagag ttcctgttag ctgtattgtt
     6481 tgattttctt attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg
     6541 cttgcacgcc ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt
     6601 ttgcccgatg cacgccatct ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt
     6661 ttccctttct atgttactgc tcatgcggtg aagttaacgc acgctcactg caggacaaca
     6721 gtaaaatcag agcgtttctg cttttactga tgtctggcgg tcggagctgg tgaccagttt
     6781 gacccatatc tcatggggca gggttttcca ccttgtccgg tattcttact tccccgaaac
     6841 gggtttgcgc ttatgaaatc aatgaatatt gccgccagta gtgaactggt atcccgactt
     6901 tcttctcatc gccgcgtggt ggcgttggga gatactgatt ttacggacgt cgcggcagtc
     6961 gtcattaccg ctgcggatag tcgcagtggc attcttgcgt tgcttaagcg caccggtttt
     7021 catctaccgg tgtttttgta ttccgaacat gctgttgaat tacctgcggg cgttacggcg
     7081 gtaatcaacg gcaacgagca gcagtggctg gagctggaat ccgcagcctg tcagtatgaa
     7141 gagaatttgc tgccaccgtt ttatgacacg ctgacgcagt acgttgagat gggcaacagc
     7201 acctttgctt gccctggaca tcaacatggt gcgttcttta aaaagcatcc tgccggacgc
     7261 catttttacg atttctttgg tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta
     7321 aaattgggcg atctgcttat tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc
     7381 aaagtctttc atgccgataa aacctatttt gtgctgaacg gcacatcggc agcgaataaa
     7441 gtggtgacga atgcgctgtt aacgcgtggc gatctggtgc tcttcgaccg taacaaccat
     7501 aagtcgaatc atcacggcgc gctgattcag gcggggacga cgccggtcta tctggaagct
     7561 tcacgtaacc cgtttggttt cattggcggt attgatgcgc actgttttaa tgaagagtat
     7621 ctgcgccagc aaattcgcga cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc
     7681 ctgacgatta ttcagctggg aacctatgac ggcactgtct ataacgcccg tcaggtgatc
     7741 gataccattg ggcatctgtg tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa
     7801 cagtttatct cgatgatggc ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat
     7861 ccggggattt ttgtgaccca gttggtgcac aaacagcagg ctggattctc acagacgtcg
     7921 cagatccata aaaaagataa ccatatccgc ggacaggcgc gtttttgccc gcataagcgg
     7981 ttgaataacg cctttatgct ccatgcttct accagccctt tctatccgct gtttgctgca
     8041 ctggatgtta acgccaaaat tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc
     8101 gttgagttgg gtattgagtc gcgcaaggct attcttgcgc gctgtaagct gttccgcccg
     8161 tttatcccgc ccgttgttga tggcaaattg tggcaggatt atccgacatc agtgttagcc
     8221 agcgaccgcc gttttttcag ttttgagccg ggggcgaagt ggcacggctt tgaaggatat
     8281 gccgcggatc agtattttgt tgatccgtgc aagctgttac tcactacacc aggtatcaat
     8341 gccgaaaccg gcgaatatag cgactttggc gttccggcga cgattctggc gcactatctg
     8401 cgtgagaacg gcattgtgcc ggagaagtgc gatctcaact ccattctgtt cttattaact
     8461 ccggcggaaa gccacgagaa gctggcacaa ctggtggcga tgctggcgca atttgaacag
     8521 catattgagg atgactcgtc gctggctgag gtgttgccga gcgtttataa caaatatccg
     8581 gtgcgctatc gcgactacac cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc
     8641 agtttcgacg tcaaagacct acaaaaagcg atgttccgcc agcagagttt cccgtcagtg
     8701 gtgatgaacc cccaggatgc gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg
     8761 attcgtgatg ccgaagggcg aattgcggca gaaggggcgt tgccttatcc acctggcgtg
     8821 ctttgcgtgg tacccgggga agtctggggt ggggcggttc aacgttattt ccttgcgctg
     8881 gaagaaggcg tgaatttgtt gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa
     8941 accgatgcgg atggcatgaa acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac
     9001 gggtcacctt ctggcgaccc gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc
     9061 tgtcgggaca cgaacgtgtt tatatttgaa catcgccatg aacgcgaagg ccagaaccac
     9121 ggaataacca gcgaaaatca accatacggt ctgccagtcg gtaatgccgt tttgggtgta
     9181 catctcaaca actttaccgc tcacgatgcc gccgaggata cagccgaagc cgttagtcat
     9241 catcaggaac atcccttgtg cactggcgcg aattgccggg ctaacttctt tttcgacaaa
     9301 caccgaacca gagatgttga agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac
     9361 cagcagtaca gtaccgaacg gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat
     9421 ccacgccaca atactgatca tcattacgtt cttaataccg tagcggctta agaagaacgg
     9481 gatggtcaga atgaacaggg tttcagagat ctgcgaaatc gacatgatga ttgacgcatg
     9541 ctgcacgatg aagctgctgg caaacatcgg atctttgtcg aagctgtgca ggaaggtatt
     9601 accgaacatg ttggtaatct gcagttccgc gcccagcagc attgagaaga tgaagaagat
     9661 tgccatacgc ttgtttttaa acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct
     9721 ctgattcgct tgctgtttag caaccggaat atgcggcaga gtcagggtaa acagaaccag
     9781 aatggcggaa agtgctgcgc caatatacag ctgcatgtgg cttaattcga agccagacag
     9841 gctcaccacc cacattgcca tgataaagcc gatggtgccc cagatacgga ttggcgggaa
     9901 gtcagtaacg atatccatcc cggcattttg caggcgatag taagagatgg tgttgattaa
     9961 cccaagcgtt ggcatataag caaacgagtt aatcaatatc acaaggaaca tcgcttccgg
    10021 cgtcgtgacc tgtgccgcca tgaacagcgt gatagcgcca atggtgtggc aaatggcata
    10081 tacccatttc gcacttaacc atttgtcggc cacaatcccc agcagcgcag gcataaatac
    10141 cgcggcgata cccagtgagc tataaactgc gccaatagaa gcaccgtcaa acttcagggt
    10201 aacaaacata taggagccga gggtcgtcag ccaacttccc cacagacaga actgcagaaa
    10261 agagaggatt ttcagctgca gcttaagatt catgttaatt tcctcacatc gtgatgcgga
    10321 tgagtgtttg aaaaagcaca tttgaccgtg cgagatgatg ggattctatc aacttcgcgg
    10381 actggttttt tgttacctgt ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat
    10441 tgtgatgtgg ataacatttt tgcccctgag catcgtcagg ggcggttaat ggaattatcg
    10501 gcggcggtag gatttttgcg cggtattcac tttataaaga taacggcgag attccgcaga
    10561 gggatggcgg gtcgtcagcg tctgataaac atcgcccggc gtcatggtgt taataatatt
    10621 ggcagcctga atcttatcat tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata
    10681 ggcggtgatg acggcataac gccgcgatgt tgggttatca attccgccga gataaacatt
    10741 gttcagcatc gccagatacg cggtgccggt atcaatattg ctggcaggat caaacaagaa
    10801 actgcggctc ggcgtgccgg atttcccctg cgagcggaac acatctttcc cggcagtatt
    10861 ttgtaccacc tgcattaatc ccagcgcatc ggaacggctg accgcatacg ggttaaagga
    10921 agattcagtc tgcataattg ccagaatcag cgactcatca acgccatatt tccgtgacgc
    10981 ctggcggacc atgccgagat atttgtgcgc acgtttatca aggtggttcg gcaccatgtt
    11041 aatggtgacg ctgtagatga tacgtagccc gttgctgcgg cttttcagac ggtttttcag
    11101 cagataatcc gcgaagttgc ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc
    11161 caccacctga ccgtaaagga aaggttcttt cgaaattgta atatcatcaa cgtcggaata
    11221 gagatcgacc gaactcggat cgtcacccat cagcaacgtt ttgataattg cccggcgcaa
    11281 atgcgcggca ggttctgtcc cggcgatggt ttcgatagta atcgtaccgt catcgaagtt
    11341 gatgtggctg cgggtctgat attgatcggt gtatttcacg tagtccttag gaccggcgat
    11401 caccacctct ttgaagcccc agatgttctc aatattgtgg gcaaattgcc ccatcagaat
    11461 atcaaaaccg ttggtatctt tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt
    11521 cgaacaggag atgagcaacg gcgcaatcaa agccagcgcg agatattttt tcatcattcc
    11581 gggtgcgtgt tgtgtttgtt tttatgcaag ctgccggagg cgtgctccgg cactgttttt
    11641 attttttatc ttccggcgta tagccctcga tatgcacctc tttaccctcg aacaggaagt
    11701 tgaccatctc ctgctccagc agcttgcggt gctcggcatt catcatgttg agtttctttt
    11761 cattaatcag catggtttgt ttgtgctgcc actgcgccca ggcttctttg gagatctcgt
    11821 tatagatgcg ttttcccagc tcgccggggt acagctgaaa atcctgacct tctgcttcac
    11881 gttgcaggaa agtacaaaaa atcgttctgc tcataaatca tcctctttat cgactcacgc
    11941 gctaaaccgg cgcgccagtg cgtaactgct gtaacaaacg ctccacggga gccgccaggc
    12001 caactgacgg cggttgcgct aagttatacc agagcgcatt gccttcatcc atgcagccgg
    12061 tgaatgacga cacgggaagc cacataggca caatatctaa gtggaaatgg ctgaaggtat
    12121 ggcgaaacgc ggtcagttgc gtcaggttat cggcagcaat ctgccgttgt gccagccaat
    12181 gccgcaaact ttcttcgtcg gcaaactgcg ggaaacagta taaaccgccc cacaatccgc
    12241 tcggcggacg ctgcgccagc aatacttcat cttcgtgctg taacaggaga aagtagccgg
    12301 tgcgctccgg cagcgtctgt ttcggttttt tgcccggata aagcgaccag ctattgttgg
    12361 tggcggcaat acatccgttt tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc
    12421 aaatcatcgc gcccaaatcc atcatcgcct gattaaaccg ttccacgccg accgcgggcg
    12481 tcacctgctc gctcaaactc catagtttat tctcgacctc ttttttccca ggccagccgc
    12541 ttacagcata gcagcgcgcc agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct
    12601 tacccagaga aagcgagaga atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg
    12661 cgacttcttc aaaggtttcc gggaatttac caccgtgtaa ggtcgccact tgttgtgccg
    12721 ctttatgcag gttgcgcgcg cgggcgtaat agccaagccc ggtccacaag tggagaactt
    12781 cgtcgagcgg cgcattggcg agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa
    12841 aataggggat aacggtcgca acctgagttt gttgcaacat cacttctgag agccatactt
    12901 tgtagggcgt cttgtcaatt tgccagggca gcgttttccg cccgtattta tcgtaccagt
    12961 ccagaacctg ggctgaaaat tgcgacgctt gcatggtcac cgacttcact gttgttgggg
    13021 gcaagattgc agcacagcga cggcagggtg taaaccggaa ctttccgcag ccacggcctt
    13081 atcatgcttg catccggcaa ctaactttgg ataatgcccg ttttcagaac actttcacaa
    13141 gcgactaaat ctttatgaaa aacgacgtca tttcaccgga atttgatgaa aacggccgcc
    13201 cactgcgccg tatccgtagt tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac
    13261 atgcgctgga aaactactgg ccggtgatgg gcgttgagtt cagtgaagat atgctggatt
    13321 tccccgcgct ttttggccgt gaagcgccgg tgacgcttga gattggtttt ggcatggggg
    13381 cgtcgctggt ggcaatggct aaagatcgcc ccgagcagga cttcctcggc attgaagtgc
    13441 attcaccggg cgttggtgcg tgcctggctt ctgcgcatga agaaggttta agcaacctgc
    13501 gcgtgatgtg tcacgatgcg gttgaagtgc tgcataaaat gattcctgac aattcattgc
    13561 gcatggtgca gctctttttc cctgacccgt ggcacaaagc gcgccataat aaacgccgta
    13621 tcgttcaggt gccgtttgcc gaactggtaa aaagcaaact gcagctgggg ggcgtattcc
    13681 atatggcgac cgactgggaa ccttatgcgg aacatatgct tgaagtgatg tcttctattg
    13741 acggttataa aaacctgtca gagagcaatg attacgtacc gcgtccggca tcacgtccgg
    13801 tgacgaaatt tgaacaacgt ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg
    13861 agagggtgaa ataatggcaa agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga
    13921 cgaattccag gaattaggat tttcggtggc atggcgattc ccggaaggta catcggaaga
    13981 acagattgat aaaatcgttg atgactttat taacgaggtt atcgaaccga acaaactggc
    14041 ctttgacggc agcggttatc tggcctggga aggtctgatc tgcatgcagg aaatcggcaa
    14101 atgcaccgaa gaacatcagg cgattgtgcg taagtggctg gaagagtgca aactggatga
    14161 ggtacgcacc agcgaacttt tcgacgtttg gtgggactaa gaaagcatac gggcgatgac
    14221 aaatgcaaaa ctgcctgatg cgctacgctt atcaggccta cagagatgca cgatcgagta
    14281 ggcgggataa ggcgtttacg ccgcatccgg catggaaaac gcgtactttg ttatcaatct
    14341 ggggccagca tatgctggcc tgatttgttc ttgagggaag actatgatgc gcaaaatgct
    14401 gctggcggca gcactttcag tgacggcaat gaccgctcac gccgactacc agtgcagcgt
    14461 cacgccgcgt gacgatgtga ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg
    14521 caatctggtg atcacgccag acggcaacgt gatgtataac ggtaagcaat attccctgaa
    14581 tgccgcccag cgcgagcagg cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg
    14641 gattgatgaa ggcgcgaaaa gccgcgtcga gaaagcccgt attgcgctgg ataaaattat
    14701 cgttcaggag atgggcgaca gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca
    14761 gcttaaagag cagatgaatc gcattatcga aacgcgcagc gatggcctga cgttccacta
    14821 taaagccatt gatcaggttc gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg
    14881 aattttacag gacagcatta atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa
    14941 cccattacag aatgtgctgg gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg
    15001 gaaaaagcag gaaaaagatt tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac
    15061 tctggaagat agccgcaaag ccctggtcgg gaatttaaaa taatcctcta ttttaagacg
    15121 gcataatact tttttatgcc gtttaattct tcgttttgtt acctgcctct aactttgtag
    15181 atctccaaaa tatattcacg ttgtaaattg tttaacgtca aatttcccat gcaaagctaa
    15241 gggataatgc gtagcgtcca cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg
    15301 cacttgccgc actggttatg ggttttagtg gtgcagcatt ggcattaccc aatatcacca
    15361 ttttagcaac cggcgggacc attgccggtg gtggtgactc cgcaaccaaa tctaactaca
    15421 cagcgggtaa agttggcgta gaaaatctgg ttaatgcggt gccgcaacta aaagacattg
    15481 cgaacgttaa aggcgagcag gtagtgaata tcggctccca ggacatgaac gataatgtct
    15541 ggctgacact ggcgaaaaaa attaacgccg actgcgataa aaccgacggc ttcgtcatta
    15601 cccacggtac cgacacgatg gaagaaactg cttacttcct cgacctgacg gtgaaatgcg
    15661 acaaaccggt ggtgatggtc ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc
    15721 cattcaacct gtataacgcg gtagtgaccg cagctgataa agcctccgct aatcgtggcg
    15781 tgctggtggt gatgaacgac accgtactgg acggtcgcga tgtcaccaaa accaacacca
    15841 ccgacgtagc gaccttcaag tctgttaact acggtcctct gggatacatt cacaacggta
    15901 agattgatta ccaacgtacc ccggcacgta agcacaccag cgatacgcca ttcgatgtct
    15961 ctaagctgaa tgagctgccg aaagtcggca tcgtttataa ctacgctaac gcatccgatc
    16021 ttccggctaa agcactggta gatgcgggct atgatggcat cgttagcgct ggtgtgggta
    16081 atggtaacct gtataaatcc gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg
    16141 cagtagtgcg ttcttcccgc gtaccgacgg gtgctaccac tcaggatgct gaagtggatg
    16201 atgcgaaata cggcttcgtc gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc
    16261 tgcagctggc tctgacgcaa accaaagatc cgcagcagat ccagcagatt ttcaatcagt
    16321 actaatcacc tcgtcccggc atcgtgccgg ggctttttca cttcagactt acgtccattg
    16381 ccaattttca ttaccctaat gataatcacc ggaataaatt attccgcgcg agggttttcg
    16441 ggtgaaaaag caatggattg ttggcacggc gctgcttatg ttgatgactg gtaatgcctg
    16501 ggcagatggc gaaccgccaa ctgaaaatat cttaaaagat caattcaaaa agcagtatca
    16561 cggcattctc aagcttgatg ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc
    16621 cacctggtca gcggaaggcg atgtctcttc cagtgacgat ctctatacct gggtcggtca
    16681 gttggcagat tacgaactgc tcgaacagac ctggacgaaa gataaaccgg taaaattctc
    16741 ggcgatgtta accagtaaag gaacgcccgc gtctggctgg tcggtgaact tttactcttt
    16801 tcaggcggca gccagcgatc gtgggcgggt ggtcgacgat atcaaaacga ataataaata
    16861 tctgatcgtg aatagcgaag atttcaatta tcgctttagt cagcttgaat ctgcgttgaa
    16921 tacccagaag aactcgattc ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat
    16981 ggtggcggcg cagaaagcag cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat
    17041 gacccgcgaa gaagccttta aaaaaattca ccaacagcgt gatgagttta ataaacagaa
    17101 cgatagcgag gcgttcgcgg ttaaatatga caaagaggtt tatcaaccgg cgattgcggc
    17161 atgtcataaa cagagtgaag agtgttatga agtgccgatt cagcagaaac gagatttcga
    17221 tatcaacgaa caacggcgac agacttttct gcaatcacaa aaactcagcc gtaaattgca
    17281 ggatgactgg gtaacgctcg aaaaagggca atatccgtta acgatgaaag tttcagaaat
    17341 aaacagtaaa aaagtcgcga ttttgatgaa aattgacgat atcaaccaag ctaatgaacg
    17401 ctagaaaaaa gataccgaac aactgcggcg caatggggtg attaagtaac tcatgttaag
    17461 tgcctttgcc ggataatata aaaagttatc cggcaaatta ttttttacat tagccctagt
    17521 agctgtggaa tgaacaagga tatttgcgga atgtaagtga tcatcatcaa cgctactagc
    17581 aatgcagcat aaaatagcag caatggtttg attaaatgtt gaatcttcac acccgagata
    17641 gaacaaccaa caaatagcgc actacccacc ggcggtgtta acagcccaat acaaaggttg
    17701 gccaccatca taataccgaa atgtactggg tccattccca gctcttgtgc aatcggcaag
    17761 aagatcggtg taaagattag tacagccggt gtcatgtcca taaagatgcc gacaattaat
    17821 aagacgatat taatcagcag caatatgatc agtgggttat cagaaatccc catcaatgcg
    17881 tcgctgatca tataaggaat atcagcatta gtcattgccc aggacatgcc cacggaaaaa
    17941 ccgatcaaca gtaatacaat cgaagtggta acaaccgact ccagaatcag tttgggtaaa
    18001 tggcgccatt ttacttcccg gtaaatcagc accgagagaa taaatgtgta gacaacggca
    18061 attgctgacg cttcagttgc ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac
    18121 accagaagca aacttggtaa ggcatccaga aacgctttac aggcctgagc aaaggttgct
    18181 ctttctgaaa gcgggtagcc acggcgttta gcaataatgc cgcagactat cattaccgca
    18241 agacccatta agatccctgg caaatatccc gccataaaca gcgatgctac agaaacgccg
    18301 ccagctgtca gggaaaacac aattaacacg tttgagggcg gtatcagtaa tccagtaata
    18361 caagatgaga cgtttacagc ggtagaaaat gccggatcat aaccttcttt ggtctgaatg
    18421 ggattcagtg tgccaccaac ggcagcagct gccgcaacgg ctgaaccaga aatggaacca
    18481 aacatcatat tcgccaggac gttcacatga ccaagcgaac caggaacgcg gccaaccatt
    18541 acctgggcga ggttaatcaa acgtatcgca ataccgccgc tgttcatcag tgttccggca
    18601 aagatgaaaa aggggattgc caacagggca aagttatcca gtccattagc cagtcgttgc
    18661 gaaacagtga ttgccgcgat atcccaggga aacatcaaaa gcattgaagc tacagtggca
    18721 ataccgattg agaacgaaat gggcacacca ataaagacca gcacaaaaaa acagccaaac
    18781 agtgtaatcg cgatataact atcc
//
LOCUS       DAJTQQ010000077        18051 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000077 DAJTQQ010000000
VERSION     DAJTQQ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18051
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.077"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            445..1128
                     /locus_tag="OO309_002880"
     CDS             445..1128
                     /locus_tag="OO309_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5847959.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            1172..5834
                     /locus_tag="OO309_002881"
                     /pseudo
     CDS             1172..5834
                     /locus_tag="OO309_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            6202..7857
                     /gene="lldP"
                     /locus_tag="OO309_002882"
     CDS             6202..7857
                     /gene="lldP"
                     /locus_tag="OO309_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR5847960.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7857..8633
                     /gene="lldR"
                     /locus_tag="OO309_002883"
     CDS             7857..8633
                     /gene="lldR"
                     /locus_tag="OO309_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR5847961.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8630..9820
                     /gene="lldD"
                     /locus_tag="OO309_002884"
     CDS             8630..9820
                     /gene="lldD"
                     /locus_tag="OO309_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR5847962.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            10016..10489
                     /gene="trmL"
                     /locus_tag="OO309_002885"
     CDS             10016..10489
                     /gene="trmL"
                     /locus_tag="OO309_002885"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR5847963.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10542..11363)
                     /gene="cysE"
                     /locus_tag="OO309_002886"
     CDS             complement(10542..11363)
                     /gene="cysE"
                     /locus_tag="OO309_002886"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR5847964.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11443..12462)
                     /gene="gpsA"
                     /locus_tag="OO309_002887"
     CDS             complement(11443..12462)
                     /gene="gpsA"
                     /locus_tag="OO309_002887"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR5847965.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12462..12929)
                     /gene="secB"
                     /locus_tag="OO309_002888"
     CDS             complement(12462..12929)
                     /gene="secB"
                     /locus_tag="OO309_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR5847966.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12992..13243)
                     /gene="grxC"
                     /locus_tag="OO309_002889"
     CDS             complement(12992..13243)
                     /gene="grxC"
                     /locus_tag="OO309_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR5847967.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13385..13816)
                     /locus_tag="OO309_002890"
     CDS             complement(13385..13816)
                     /locus_tag="OO309_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR5847968.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            14061..15605
                     /gene="gpmM"
                     /locus_tag="OO309_002891"
                     /gene_synonym="pgmI"
     CDS             14061..15605
                     /gene="gpmM"
                     /locus_tag="OO309_002891"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR5847969.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15615..16898
                     /gene="envC"
                     /locus_tag="OO309_002892"
     CDS             15615..16898
                     /gene="envC"
                     /locus_tag="OO309_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR5847970.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16902..17861
                     /locus_tag="OO309_002893"
     CDS             16902..17861
                     /locus_tag="OO309_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR5847971.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17848..18051)
                     /locus_tag="OO309_002894"
                     /pseudo
     CDS             complement(17848..18051)
                     /locus_tag="OO309_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4547 a         4664 c         4674 g         4166 t
ORIGIN      
        1 attcactccc tttacttcga tttaccgctg gttgatattc tgaaaatgca tttttaccga
       61 aatagccatc tataaacaat aaaaatatat agaagatatc atttgttaat taacaagcat
      121 ttacgtctct ttttttcgga tttaattgtg aaattttaat catcatattc acaaaatcaa
      181 ttatctgtga aatttttccc agacatggtt tctttcatat ttgacattta ttaataatca
      241 tcattagaat ccacgaaaaa taatatattc atacaaacac agacatcttt tgaaacgaat
      301 gatacatttt tatgatgaat gcttaaaaca atatttaatc gacacacatc actgtcatta
      361 atgtaaagca acatagctca ccaataatac aatggacaac tctaccggca ttgagcgctg
      421 gtcggaggaa ataaggattt ttacatgaac ttgaagaaga tttttttcag tgctgtcact
      481 gtttcagttc tctgcgcatt aacgggatgt gactacattg aagaaggcaa gccagaaagt
      541 agtttactga aacaacaaga agaacataat aataaaattg tgctacttga aaaacaacaa
      601 gcacagctaa agagtcaact cgaaactatt caaaaacaac aaactggcat aataaacagt
      661 acaaagactt taactcacgt aattaaatcc gttaaagacc aacaaaatac ttttattttt
      721 accgagttta acccagcaaa aacgaagtat ttcatcctga acaacggttc ggtggcttta
      781 gcgggtcgag tgttatccat tgacgccaca gagaatggta gtgttattca tatctcactg
      841 gtcaacttat taagtacacc tatctcaaac attggtttta atgcgacatg gggtggtgaa
      901 aaacctgtcg atgccaaaga gtttgcccgg tggcaacaat tacttttcaa cacatcaatg
      961 aaatccacat tgaaattatt accaggtcaa tggcaagaca ttaatttgac cctgaagggt
     1021 gtatcgccca ataacctggg atatctgaag ttagccatca acatggaaaa tattcagttc
     1081 gacaatcttc cctctgctga aaatcggcag aaaagaagca aaaaataaat gcagttagta
     1141 cgcttataac aaggaatagc gagcatcatt aatgaacaaa atatttaaag ttatctggaa
     1201 ccctgcgaca gggaattata ctgttaccag cgaaacggca aaaagccgtg gcaagaaatc
     1261 tgggcgcagt aagctgttaa tttctgcgct ggttgcgggt ggaatgttgt cgtcgtttgg
     1321 ggcattggcg aatgccggga atgacaacgg tcagggtgtt gattacggta gtggatcagc
     1381 tggcgacggc tgggttgcta taggcaaagg ggcgaaagca aatactttta tgaacaccag
     1441 tggttccagt actgctgtgg gttatgacgc tatagctgaa ggccaatata gctctgccat
     1501 cgggtcaaaa acccatgcga ttggtggtgc atcaatggcc tttggggtta gtgcaatatc
     1561 agaaggcgat agaagtatag cactgggtgc ctcttcgtat tcattgggcc aatactcaat
     1621 ggccctcggc cgttattcaa aagcattggg taaattgtct attgctatgg gggactcttc
     1681 caaagcggaa ggagcaaacg ccattgccct ggggcgtagc agtgtagcta gcggtactga
     1741 cagcctcgca tttggcagac aatcacttgc cagcgcagcg aacgctattg cgataggtgc
     1801 tgagaccgaa gccgctgaaa atgcaactgc tattggcaat aatgcgaagg caaaagggac
     1861 taatagcatg gcaatggggt tcggaagcct tgccgataaa gtcaatacta tcgcattagg
     1921 aaatggcagc caggctctgg cagataatgc aatcgccata ggccagggca acaaagctga
     1981 tggcgtggat gccatcgctc tgggtaatgg tagccagtcg agaggcttaa acaccattgc
     2041 cttaggcaca gccagtaatg caactggtga taagagtctt gcgcttggta gtaatagcag
     2101 tgccaacggt attaactctg tcgcgctggg cgcagattcc attgcggatt tagacaatac
     2161 cgtctctgtc ggcaatagtt cattaaaacg caagatcgtt aatgtgaaaa atggcgcgat
     2221 caagtctgac agttacgatg ccattaatgg ttcacagctt tatgccatta gcgactcggt
     2281 agcaaaaaag gcttggagga ggggctgcag tagatgttga tgacggtact gttacagcac
     2341 caacctacaa tttaaaaaat ggtagcaaaa ataacgtagg ggctgcgctc gctgtacttg
     2401 atgaaaacac cctgcaatgg gaccaaacca aaggcaaata cagcgctgct catggtacta
     2461 gtagcccaac tgccagcgta atcaccgatg ttgcggatgg cacgatttca gcctccagta
     2521 aggatgcggt taacggttcc caactgaaag ctaccaatga cgatgtcgaa gccaacacca
     2581 ccaatatcac caacctgacg gattccgttg gtgaccttca ggctgatgcc ctgctctgga
     2641 acgaaactaa aaaggcattc agtgcagctc acggccagga taccaccagc aaaatcacca
     2701 acgttaaaga tgccgacctg acggctgaca gcactgatgc tgttaacggc tctcagctga
     2761 aaaccaccaa cgatgctgtg gcgacgaata ccaccaatat cgccaataac acttccaata
     2821 ttgccactaa caccaccaac atctctaacc tgactgagac ggtgactaat cttggtgagg
     2881 atgcgctgaa atgggataag gacaatggtg tattcacggc agctcatggc accgagacca
     2941 ccagcaaaat aaccaacgtt aaagatggcg acctgacgac tggcagcacc gatgccgtta
     3001 acggctctca gctgaaaacc accaacgatg ccgtggcgac gaataccacc aatatcgcca
     3061 ctaacaccac caacatctct aatctgactg agacggtgac taatcttggt gaggatgcgc
     3121 tgaaatggga taaggacaat ggtgtcttca ctgcagctca tggcaacaat accgccagca
     3181 aaatcaccaa tatcctggac ggcacagtca ctgcaaccag ttccgatgcc attaacggta
     3241 gccagcttta tgacttaagc agcaatatcg ccacctactt cggcggcaat gcttctgtga
     3301 atactgacgg tgtgtttacc ggtccaacct acaaaatcgg tgaaacaaat tattataacg
     3361 tcggcgatgc actggctgcg attaactcct catttagcac gtctctcggc gatgctctgc
     3421 tttgggatgc caccgcaggt aaattcagtg ccaaacacgg tactaatggt gacgcaagcg
     3481 tgatcactga tgtcgcagat ggtgaaattt cagactccag ttctgacgca gtaaacggct
     3541 cacaactcca cggcgtgagc agttatgttg ttgatgcgct ggggggtggt gccgaagtca
     3601 atgcagacgg caccatcact gcgccgacgt acaccattgc taatgctgat tacgataatg
     3661 tcggtgatgc cctgaatgct atcgatacca ctcctgacga cgctctgctc tgggatgcgg
     3721 acgccggtga aaatggtgca tttagcgccg ctcacggaaa agataaaact gccagtgtaa
     3781 tcactaacgt cgctaacggt gcaatctctg ctgccagcag cgacgcgatt aacggctcac
     3841 aactctatac caccaataag tacatcgctg atgcgctggg tggtgacgca gaagtcaacg
     3901 ctgacggcac catcaccgca ccgacttaca ccattgcgaa cgccgagtac aacaacgtcg
     3961 gtgacgccct ggatgcgctt gatgataacg ccctgctgtg ggataagact gccaatggcg
     4021 gtgctggagc ctacaatgcc agccatgacg gtaaagccag catcatcact aatgtcgcta
     4081 atggcagtat tagtgaggac agtaccgatg cagtgaacgg ttctcagttg aatgcgacga
     4141 atatgatgat tgagcagaac acccaaatta tcaatcagct cgctggtaac accgacgcaa
     4201 cctatatcga agaaaatggc gctggtatca actacgttcg tactaacgac aacgatttag
     4261 cctttaacga tgcaagcgcc tctggtgtcg gcgctacagc tgtaggttat aacgctgtcg
     4321 cgtctggtgc cagcagcgta gccattggtc agaacagcag cagcaccgtt gataccggta
     4381 ttgcgctggg tagcagctcc gtttccagcc gtgtgattgc caaaggttct cgtgacacta
     4441 gcgtaacgga aaatggcgtg gtgattggtt atgacaccac tgacggcgaa ctgctaggtg
     4501 cattgtcaat tggtgatgac ggtaaatacc gccaaatcat caacgtagct gatggttcag
     4561 aagcccatga cgccgttacg gttcgccagt tgcagaacgc tattggagcg gtcgccacta
     4621 cgccaaccaa gtactttcac gccaactcaa cggcagaaga ctcactggcc gttggtgaag
     4681 actcgctggc aatgggtgcg aaaactgtcg ttaatggtaa tgcaggtatt ggtatcggtt
     4741 tgaacactct ggttctggct gatgcgatca acggcattgc tatcggcagt aacgcacgcg
     4801 caaaccatgc aaacagtatc gcaatgggta acggttctca gaccacccgt ggtgcacaga
     4861 ctggctacac cgcctacaac atggacgcac cgcagaactc tgtaggtgag ttttctgtcg
     4921 gtagtgaaga cggtcaacgt cagatcacaa acgtcgcagc tggttcagcg gataccgatg
     4981 cggttaacgt gggtcagttg aaagtcactg atgagcgcgt agcgcaaaat acccagagca
     5041 ttactaacct gaacaatcag gtcactaatc tggatactcg cgttactaat atcgaaaacg
     5101 gtattggcga cattgtcacc accggtagca ccaagtactt caagaccaac accgatggcg
     5161 tagatgccaa cgcccagggt aaagatagcg ttgctattgg ttctggttcc attgctgccg
     5221 ctgacaacag cgtcgcactg ggtaccggtt ccgttgcaga ggaagaaaat acaatctctg
     5281 taggttcttc tactaaccaa cgccgtatca ccaacgtagc tgccagtgtt aatgccaccg
     5341 atgcggttaa cgtgtcgcag ctgaaatctt ctgaagcagg cggagtgcgc tacgacacca
     5401 aagctgatgg ttctatcgac tatagcaata tcaccctcgg tggtggcaac ggcagtacga
     5461 ctcgtatcag caacgtctcc gctggcgtca acaacaacga cgcggtgaac tacgcgcagt
     5521 tgaagcaaag cgcgcaggaa acgaagcaat acaccgatca gcggatggtt gagatggata
     5581 acaaactgtc taaaactgaa agcaagttga gcggtggtat cgcttctgca atggcaatga
     5641 ccggtctgcc gcaggcttat acaccgggtg ccagcatggc ttctattggt ggcggtactt
     5701 acaacggtga atcggcagtt gctttaggtg tatcgatggt gagcgccaat ggtcgttggg
     5761 tctacaaatt acaaggtagt accaatagcc agggtgaata ctccgccgca ctcggtgccg
     5821 gtattcagtg gtaatgatcc attaagttag tgtgactaag ggcaaacgcc aaattgcctg
     5881 atgcgttacg cgcatcaggc aagataaggc gctccgggtc aacaatctaa gggcaattct
     5941 ctgatgagga ttgccctttt ctttaccaga catctccccc cacaagaatt ggccctacca
     6001 attcttcgct tatctgacct ctggttcaca attccccaat taaaactcac atcaatattg
     6061 ccaatacata acatttagtt aaccattcat tgtcattatc cctacacaac acaattggca
     6121 gtgccacttt tacacaacgt gtgacaagga gatgagcaac agactcatta cacgatgtgc
     6181 gtggactcta ggagacctgc aatgaatctc tggcaacaaa actacgatcc cgccgggaat
     6241 atctggcttt ccagtctgat agcatcgctt cccatcctgt ttttcttctt tgcgctgatt
     6301 aagctcaaac tgaaaggata cgtcgccgcc tcatggacgg tagcaatcgc ccttgccgtg
     6361 gctttgctgt tctataaaat gccggtcgct aacgcgctgg cctcggtggt ttatggcttc
     6421 ttctatggtt tgtggcccat cgcgtggatc attattgcag cagtgttcgt ctataagatc
     6481 tcggtgaaaa ccgggcagtt tgacatcatt cgctcgtcta ttctttcgat aacccctgac
     6541 cagcgtctgc aaatgctgat cgtcggtttc tgtttcggcg ctttccttga aggagccgca
     6601 ggctttggcg caccggtagc aattaccgcc gcattgctgg tcggcctggg ttttaaaccg
     6661 ctgtacgccg ccggactgtg cctgattgtt aacaccgcgc cagtggcatt tggcgcgatg
     6721 ggcattccaa tcctggttgc cggacaagta acaggcatcg acagctttga gattggccag
     6781 atggtggggc gtcagctacc gtttatgacc attatcgtgc tgttctggat catggcgatt
     6841 atggacggct ggcgcggtat caaagagacg tggcctgcgg tcgtggttgc aggcggctcg
     6901 tttgccatcg ctcagtacct tagctctaac ttcattgggc cggagctgcc ggacattatc
     6961 tcttcgctgg tatcactgct ctgcctgacg ctgttcctca aacgctggca accagttcgc
     7021 gtattccgct tcggtgactt gggggcgtca caggttgata tgacgctggc ccacaccggt
     7081 tacaccgcgg gtcaggtgtt acgtgcctgg acaccgttcc tgttcctgac cgctaccgta
     7141 acactgtgga gcatcccgcc gtttaaagcc ctgttcgcat cgggtggtgc gctgtatgag
     7201 tgggtgatca acattccggt gccgtacctc aataaactgg ttgcccgtat gccaccagtg
     7261 gtcagcgagg ctacagccta tgccgccgtg tttaagtttg actggttctc tgccaccggc
     7321 accgccattc tgtttgctgc actgctctcg attgtctggc tgaagatgaa accgtctgac
     7381 gctatcagca ccttcggcag cacgctgaaa gaactggctc tacccatcta ctccatcggc
     7441 atggtgctgg cgttcgcctt tatctcgaac tattccggac tgtcatcaac actggcactg
     7501 gcactggcgc acaccggtca tgcattcacc ttcttctcgc cgttcctcgg ctggctgggg
     7561 gtattcctga ccgggtcgga tacctcatct aacgccctgt tcgccgcgct gcaagccacc
     7621 gcagcacaac aaattggcgt ctctgatctg ttgctggttg ccgccaatac caccggtggc
     7681 gtcaccggta agatgatctc cccgcaatct atcgctatcg cctgtgcggc ggtaggcctg
     7741 gtgggcaaag agtctgattt gttccgcttt actgtcaaac acagcctgat cttcacctgt
     7801 atggtgggcg tgatcaccac gcttcaggct tatgtcttaa cgtggatgat tccttaatga
     7861 ttgttttacc cagacgcctg tcagacgagg ttgccgatcg tgtgcgggcg ctgattgatg
     7921 aaaaaaacct ggaagcgggc atgaagttgc ccgctgagcg ccaactggcg atgcaactcg
     7981 gcgtatcacg taattcacta cgcgaggcgc tggcaaaact ggtgagcgaa ggcgtgctgc
     8041 tcagtcgacg cggcggcggg acgtttattc gctggcgtca tgacacatgg tcggagcaaa
     8101 acatcgtcca gccgctaaaa acgctgatgg ccgatgatcc ggattacagt ttcgatattc
     8161 tggaagcccg ctacgccatt gaagccagca ccgcatggca tgcggcaatg cgcgccacac
     8221 ctggcgacaa agaaaagatt cagctttgct ttgaagcaac gctaagtgaa gacccggata
     8281 tcgcctcaca agcggacgtt cgttttcatc tggcgattgc cgaagcctca cataacatcg
     8341 tgctgctgca aaccatgcgc ggtttcttcg atgtcctgca atcctcagtg aagcatagcc
     8401 gtcagcggat gtatctggtg ccaccggttt tttcacaact gaccgaacaa catcaggctg
     8461 tcattgacgc catttttgct ggtgatgccg acggggcgcg taaagcaatg atggcgcacc
     8521 ttagttttgt tcacaccacc atgaaacgat tcgatgaaga tcaggctcgc cacgcacgga
     8581 ttacccgcct gcccggtgag cataatgagc attcgaggga gaaaaacgca tgattatttc
     8641 cgcagccagc gattatcgcg ccgcagcgca acgcattctg ccgccgttcc tgttccacta
     8701 tatggatggt ggtgcatatt ctgaatacac gctgcgccgc aacgtggaag atttgtcaga
     8761 agtggcactg cgccagcgta ttctgaaaaa catgtccgac ttaagcctgg aaacgacgct
     8821 gtttaatgag aaattgtcga tgccggtagc actggcaccg gtgggtttgt gtggcatgta
     8881 tgcgcgtcgt ggcgaagttc aggcagccaa agcggcggac gcgcatggca ttccgtttac
     8941 tctctcgacg gtttccgttt gcccgattga agaagtcgcg ccagccatca agcgcccaat
     9001 gtggttccag ctttatgtac tgcgcgatcg cggctttatg cgtaacgcgc tggagcgagc
     9061 aaaagcagcg ggttgttcga cgctggtttt caccgtggat atgccgacac cgggcgcacg
     9121 ctaccgtgat gcgcattcag gtatgagcgg cccaaatgcg gcaatgcgcc gctacttgca
     9181 ggcggtgacg catccgcaat gggcgtggga tgtgggcctg aacggtcgtc cacatgattt
     9241 aggtaatatc tcagcttatc tcggcaaacc gaccggactg gaagattaca tcggctggct
     9301 ggggaataac ttcgatccgt ccatctcatg gaaagacctt gagtggatcc gcgatttctg
     9361 ggatggcccg atggtgatca aagggatcct cgatccggaa gatgcgcgcg atgcagtacg
     9421 ttttggtgct gatggaattg tggtttctaa ccacggtggc cgccagctgg acggtgtact
     9481 ctcttccgct cgtgcgttgc ccgctattgc agatgcggtg aaaggtgata tcgccattct
     9541 ggcggatagc ggaattcgta acgggcttga tgtcgtgcgt atgattgcgc tcggtgccga
     9601 caccgtactg ctgggtcgtg ctttcctgta tgcgctggca acagcgggcc aggcgggtgt
     9661 agctaacctg ctaaatctga tcgaaaaaga gatgaaagtg gcgatgacgc tgactggcgc
     9721 gaaatcgatc agtgaaatta cgcaagattc gctggtgcag gggctgggta aagagttgcc
     9781 tacggcactg gctccgatgg cgaaagggaa tgcggcatag tcgtttgcgc cccctcaccc
     9841 taaccctctc cctcagggag aggggaccgt tcggcgctgt atgtactccc tcactctgaa
     9901 acgacaccgc actctttttt tctccctcgc ccctccgggg agggccgggg tgaggggaaa
     9961 aggccgcacg gttcaccaat tttctgctat cctgcccccg cactaagggg gcagtatgct
    10021 aaacatcgta ctttacgaac cagaaattcc gccaaatact ggcaacatca tccgtctttg
    10081 cgctaatacc ggctttcgtc tacatatcat cgaaccgatg ggatttgcct gggacgacaa
    10141 gcgcctgcgc cgcgcggggc tggactatca cgagtttacc gccgttacgc gtcatcatga
    10201 ctatcgcgcg ttcctcgaag cagaaaatcc ccagcgcctg tttgccctca ccacgaaagg
    10261 tacgcctgct cacagcgccg taagctatca ggatggcgat tatctgatgt tcggcccgga
    10321 aacacgcggc ctgccagcga gcattcttga tgccctgccc gctgaacaaa aaattcgcat
    10381 tccgatggtg ccggacagcc gcagcatgaa tctgtccaat gcggtgtcgg tagtggtgta
    10441 tgaagcctgg cggcagctgg gatatccggg agcgttgttg agagattagt tactgtatgc
    10501 cggatagatg attacatcgc atccggcacg atcacaggac attagatccc atccccatac
    10561 tcaaatgtat ggttaatacc gttgaaatgc tggtccatat ccattgatgg cttatcgctg
    10621 tctggtttac cgacaatacg agccggaacg ccagcggcgg ttgtatgcgg cggcaccggt
    10681 tgcagcacca cggaacctgc gccaatcttc gcgccgcgac caacttcaat attgccgagg
    10741 attttcgcgc ccgcgccaat catcacacct tcacgaattt tcgggtgacg gtcaccacca
    10801 gatttacccg taccgccaag cgtcacagat tgcagaatcg atacgtcgtt ttcaattacc
    10861 gccgtttcac ccacaacgat gcccgtggcg tggtcgagca taatgccacg gccaattttt
    10921 gctgccgggt gaatatcgac ctggaacgtc acagaaacct ggttttgcag aaagattgcc
    10981 aatgcgcgac gcccctgatt ccacaaccag tgaccgatgc gataggcctg caaggcatga
    11041 aaacccttca ggtataacaa cggggttgag tatttatcga ctgccgggtc gcgggtacgc
    11101 accgcctgaa tatcacaggc cgcagaggcg atcatttccg ggtcagcggc gtaggcttct
    11161 tcaaccactt cacggatagc aatagcaggc ataattggcg atgacagctt gttcgccagc
    11221 atgtagctca gtgcactgcc aaggttttcg tgcttgagta gcgtcgcgtg gtaaaaactg
    11281 gccagcattg gctcacagtc cgccagcgtt ctggcttcgg ctttaatatt gttccagaca
    11341 atttccagtt cttcacacga cattgcttac tccacacgat gagataatga ccggcccgtt
    11401 ctgcgcgggc cgggtcatag cggtaacaaa ggttccctgg ggttagtgac tgctgcgctc
    11461 gtccttgcgt gcacgaccta ataatgtcaa tgctgcctcg cgcgcgtttt ttccgcaata
    11521 taatacttga taaatttcct cggttattgg catttcaacg ccgaagcgat gcgccagttc
    11581 gcggacttct ttcgtattgc ggtagccttc caccacctga ccaatcttct cctgcgcgct
    11641 ttgtacatcc atgccctgac cgagcatcat accaaaacgg cggttacgcg actggttgtc
    11701 ggtacaggta agcaccagat cgcccagccc cgccatgccc ataaaggtgg caggatcggc
    11761 acccagcgcc gcaccaagac gcgacatttc agccagccca cgggtgatca gcgccgtacg
    11821 cgcattcgca ccaaaaccga taccgtcaga catccccgcg ccaatggcaa tgacgttttt
    11881 caccgcgccg ccaagctgca cgccaatgaa atccgggttg ctgtaaacgc ggaaactttt
    11941 gccgcagtgc agcagctgct ggagatcatc ggcaaaggtc tgatcggtcg aggccagcga
    12001 aatagctgtc ggtaaacctg ccgccagttc tttcgcaaac gttgggccag agataaccgc
    12061 cagcggaatt tgatcgccta aggcctcacg cgccacgtcc tgtaacagac gtccggtttc
    12121 cgcttccagc cctttggtcg cccacaccag acgcgcatca ggacgcatca gcggtttaat
    12181 ctggcgcagc acttcaccaa agacatggct gggtacgacg acgagaatat tacgactggc
    12241 tgccagcgca gtggcgagat cgctttcaag atggagcgta tcgggaaaag gcacatcggg
    12301 gagaaacgcg gcgttacagc ggtcgcgttc aagcgttgcg atatgttcag ggtcatggcc
    12361 ccagaggaca acctcgtggc catttcttgc cagggtaatg gcaagagcgg tgccgtacga
    12421 gccggcaccg atcacagtca ttgaagcatt acgttggttc atcaggcatc ctgatgttct
    12481 tcagtacctt cgccagcctg ctgctgcaaa tagttcatga acagcgcatc gaagttaacc
    12541 ggcgcaaggt tcagttgcgg gaatgtaccg cgggatacca tgctggtgat gcactcacga
    12601 gcatacggga acagaatgtt cgggcagtat gcgcccaggc aatgtgccat ctgggtgcct
    12661 tcgatacccg cgatggagaa aataccgccc tgctgaactt cacacaggaa cgcggtttct
    12721 tcgcccaagg aggccgttac ggtaacacgc agtaccactt cgtatacgtc atctgccagt
    12781 tgggaagatg ccgtatccag atcaagttta acttctggtt gccaatcttt ctggaaaacg
    12841 tgcggcgcgt tcggcgcttc gaaagagata tccttggtat aaatacgttg gatctggaaa
    12901 gtcatttcag tgttgttttg ttctgacatg tgtagaaaac ccttaagtgt tgtccttaaa
    12961 tactgcgcaa tgtaatacca acgttcacac gttatttcag caggggatcc agtccaccac
    13021 gtgcatccaa tgcatacaag tcatcacagc cgccaatgtg ctgtgcgtca ataaaaatct
    13081 ggggaaccgt ggtgcaaccg ctgcgtttga tcatctcttc acgcttggcg gcattgccat
    13141 cgatcggcaa ctcctggaaa ctcacgccct tgctgctcag cagtgctttt gcacgatggc
    13201 aatacgggca ggtttctttg gtatagattt caacattggc cataatttag ctcctgtttt
    13261 tttaccctga aatttcatgt tgcagggcgg ctgcaaaatg gagtgtcccc agaagcttat
    13321 aacaataagt cgctgggggc ctaattgcag ctaacggcct gcatcatgaa agacgacagg
    13381 taaattattt gccgcgcacc agaggcaagt tttcgccagc ccagccagca acgccctctt
    13441 tcagcacgaa tacttgcgca aaaccagctt tcgtcagcgc gtttgcaggc tcctggcact
    13501 gcatgccaga accgtctacc acgataaccg atttgtcttt gtgcttctca agctcaccaa
    13561 cattgttggc tttgatttcg ctcggcaaca gattgataga acctgcgata tggcctttac
    13621 ggaagtcatc acgctgacgt aaatccacaa ccacagcgtc ttctttgttg atcagacgcg
    13681 tagcttcacc acgagtaatc accttcactt tcgaggtcag gctcttaaac gtagtcacaa
    13741 gaaccgccac cagtaacgcg atccaggcga tactcagtat gggatgacgg ccaacaaatt
    13801 gcataatttc ttgcatgggg ggtaacaact cccgacgtag tgattaaaaa aaccaggaaa
    13861 ggagtatacc tgcgcggtgc ggcaaataca gccagcgcgt taactggaat gcaattttgc
    13921 ggggcacgac gaaaaaaaag cgcaaatctg gcatcggctt gccgcgagcg gtcgtatatt
    13981 ttgatcttca gaggctattt tatcgattca gctgtagtaa aattacgcaa attttgactc
    14041 ttgagtatga ggttgtcgca atgtcggttt ctaaaaaacc tatggtactg gtgattctgg
    14101 atggctatgg ctatcgtgaa gaacagcagg ataacgccat ttttagtgct aaaaccccgg
    14161 taatggatgc actgtgggcc aatcgtccgc ataccctgat cgacgcttcc ggtctggaag
    14221 tcggtctgcc tgaccgtcag atgggtaact ccgaagtagg ccacgttaac ctgggtgccg
    14281 gccgcatcgt gtatcaggac ctgactcgtc tggacgttga aatcaaagat cgcgctttct
    14341 ttgctaatcc agtgctggct ggcgcggtac ataaagcgaa aaacgcaggt aaagcagtac
    14401 acattatggg tctgctctcc gcaggcggcg tacacagcca cgaagatcac atcatggcga
    14461 tggtagaact ggcagctgaa cgcggcgcag aaaaaatcta cctgcacgca ttccttgacg
    14521 gtcgcgacac cccgccgcgc agtgctgaat cctcgctgaa aaaatttgaa gagaaatttg
    14581 ctgcgctggg taaaggccgc gtagcgtcca tcattggtcg ttactacgcg atggaccgcg
    14641 ataaccgttg ggatcgcgtt gaaaaagcct atgacctgct gactctggcg cagggtgagt
    14701 tccaggccga tactgccgtt gctggtttgc aggcagctta tgctcgcgac gaaaacgacg
    14761 agttcgtgaa agcgaccgtt attcgtgcag aaggtcagcc agatgcggca atggaagacg
    14821 gcgatgcgct gattttcatg aacttccgtg ctgaccgcgc gcgtgaaatc actcgtgcat
    14881 tcgtgaacgc tgatttcgat ggcttcgcgc gtaagaaagt ggttaacgtc gatttcgtga
    14941 tgctgaccga atacgccgct gacatcaaaa ctgcggttgc ttacccaccc gcttccctgg
    15001 ttaacacctt cggcgagtgg atggcgaaaa acgacaaaac tcagttgcgt atttccgaaa
    15061 ccgaaaaata tgcccacgtt actttcttct tcaacggtgg cgtagaagag tcgttcaaag
    15121 gcgaagatcg cattctgatc aactcaccga aagtggctac ctacgatctg caaccggaaa
    15181 tgagctccgc agagctgacc gaaaaactgg ttgcggccat caagagcggc aaatacgaca
    15241 ccattatctg taactatccg aacggcgaca tggtaggtca caccggggtg atggaagcgg
    15301 cggttaaagc ggttgaagca ctggatcact gcgtggaaga agtcgcgaaa gcggttgagt
    15361 ccgtgggtgg acaactgctg atcaccgctg accacggtaa cgctgaacag atgcgcgatc
    15421 cggcaacggg tcaggcacac acggcacaca ccaacctgcc agttccgctg atttacgttg
    15481 gtgataagaa cgtgaaagcg gttgcaggcg gcaaactttc tgacatcgcg ccgaccatgt
    15541 tgtcgctgat gggtatggaa atcccgcaag agatgactgg taagccgctg ttcatcgtgg
    15601 aataatccct ccccatgagg ggaaaggcga ttaataccat gacacgggcc gtgaaaccgc
    15661 gcaggtttgc aatcaggccc atcatctacg ccagcgttct tagcgctggc gtattgttgt
    15721 gcgccttttc cgcccacgcg gatgagcgtg accaactcaa atctattcag gccgatatcg
    15781 ccgcaaaaga gcgcgcggta cgccaaaagc aacaacaacg cgcaagcctg ctcgcacaat
    15841 tgaaaaagca ggaagaagcg atctctgaag ccacccgtaa gctgcgcgaa acgcaaaaca
    15901 cgctcaatca actcaataaa cagattgatg agatgaacgc gtcgattgcc aaactggagc
    15961 agcaaaaagc cgcccaggag cgcagcctcg ccgcacaact ggatgccgca ttccgtcagg
    16021 gcgagcatac cggtattcag ctaattctca gcggtgaaga aagccagcgt ggacagcgtt
    16081 tacaggctta tttcggctat ctcaaccagg cgcgacaaga aaccattgcc cagttgaagc
    16141 aaacgcgtga agaagtcacc atgcagcatg ctgaactgga agagaaacaa agcgagcaac
    16201 aaacgctgtt atatgaacag cgcgcccaac aggcgaaact gactcaggct ctgaacgagc
    16261 gtaaaaagac gctggcgggg ctggaatctt ccatccagca aggtcagcag cagctaagtg
    16321 aactgcgtgc caacgaatcc cgtctgcgta acagcattgc ccgtgctgaa gccgcggcga
    16381 aagcgcgtgc tgaacgagaa gcgcgtgagg cccaggcggt tcgcgagcgc cagaaagaag
    16441 cgacgcgcaa aggcaccacc tacaaaccga ccgaaagcga aaaatcgctg atgtcccgaa
    16501 ctggtggcct gggtgcgccg cgtggtcagg cattctggcc ggttcgcggg ccgacgctgc
    16561 atcgctatgg cgagcagcta cagggtgaat tacgctggaa agggatggtt atcggtgcct
    16621 ctgaaggtac tgaagttaaa gcaattgccg acggtcgagt gattctggct gactggctgc
    16681 aaggttacgg tctggtggtg gtggttgagc acggtaaagg cgacatgagt ctttacggct
    16741 ataatcagag cgcactggtg agcgttggtt cgcaggttcg cgcgggccag ccaattgcac
    16801 tggtgggcag cagtggcggt cagggtcggc cttcactcta tttcgaaatt cgccgccagg
    16861 gtcaggcggt caatccacag ccgtggttgg gaagataagt tttgtttcca tttcgtcgta
    16921 acgttcttgc atttgccgct ctgttggcgc tctcctcccc cgtacttgct ggcaaacttg
    16981 ccatcgtcat tgatgatttt gggtatcgcc cgcacaacga aaaccaggtg ctggcgatgc
    17041 cttccgctat ctccgtcgct gtattacccg attcaccgca cgccagagag atggcgacca
    17101 aagcgcataa cagcggtcac gaagtgttga ttcatctccc gatggcaccg ttgagtaaac
    17161 agccgctgga gaaaaatacg ctacgcccgg agatgagcag cgacgaaatt gagcgcatta
    17221 ttcgtagtgc ggtcaataac gtgccctatg ccgtggggat caacaaccac atgggcagca
    17281 agatgacctc taacctgttt ggtatgcaga aagtgatgca ggcgctggag cgttacaatc
    17341 tttacttcct cgacagcgta accatcggta atacccaggc gatgcgcgcc gcgcaaggta
    17401 ctggcgtgaa ggtgatcaaa cggaaggtgt tcctcgacga ttcgcagaac gaagcggaca
    17461 tccgtgtgca atttaatcgc gcaattgatc tggcgcgtcg caacggttca accattgcca
    17521 ttgggcatcc tcacccttca acggtacgcg tgctacaaca gatggtttat aacctgccgc
    17581 cagacattac gctggtgaaa gccagcagct tgctgaatga accgcaggtt gatacttcta
    17641 caccgccgaa aaacgctgtg cctgatgcac cgcgtaatcc gttccgtggc gtgaagctgt
    17701 gcaaaccgaa gaaaccgata gaacctgttt atgctaatcg cttctttgaa gtattaagcg
    17761 aaagcatcag ccagagcacg ctgatcgttt acttccagca tcagtggcaa ggctggggca
    17821 aacagcccga agcggcgaag tttaacgcta gcgcaaatta aaggcgctac gcgtaatgcg
    17881 atggtgcgac cgcgttttgt cgcgccattg ccataagcgg aaagaccaca gtaacgcctg
    17941 gtaaccgacc ttcacgctgc gcacattggt aatcaggcgc ttatacatac ccggaagtaa
    18001 atatctcggc aatcatccgt tggcgggtaa gaatatccgg ctctttgcgc a
//
LOCUS       DAJTQQ010000078        18049 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000078 DAJTQQ010000000
VERSION     DAJTQQ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.078"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            246..770
                     /locus_tag="OO309_002895"
     CDS             246..770
                     /locus_tag="OO309_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5847972.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            851..1525
                     /locus_tag="OO309_002896"
     CDS             851..1525
                     /locus_tag="OO309_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5847973.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1541..4003
                     /locus_tag="OO309_002897"
     CDS             1541..4003
                     /locus_tag="OO309_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5847974.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGWNTRIGA
                     ISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVW
                     ANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGR
                     SGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDVTTPRRQ
                     IYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPV
                     ATVNGSYSQSSTYRQTGATVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQK
                     YRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTD
                     QRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQ
                     QGLSCTITFGKEIDESRNYICQ"
     gene            4019..5052
                     /locus_tag="OO309_002898"
                     /pseudo
     CDS             4019..5052
                     /locus_tag="OO309_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(5134..5472)
                     /gene="rcnB"
                     /locus_tag="OO309_002899"
     CDS             complement(5134..5472)
                     /gene="rcnB"
                     /locus_tag="OO309_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR5847975.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5691..6521)
                     /gene="rcnA"
                     /locus_tag="OO309_002900"
                     /pseudo
     CDS             complement(5691..6521)
                     /gene="rcnA"
                     /locus_tag="OO309_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6643..6915
                     /gene="rcnR"
                     /locus_tag="OO309_002901"
     CDS             6643..6915
                     /gene="rcnR"
                     /locus_tag="OO309_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR5847976.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      7007..7103
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7138..7926
                     /gene="thiM"
                     /locus_tag="OO309_002902"
     CDS             7138..7926
                     /gene="thiM"
                     /locus_tag="OO309_002902"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR5847977.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7923..8723
                     /gene="thiD"
                     /locus_tag="OO309_002903"
     CDS             7923..8723
                     /gene="thiD"
                     /locus_tag="OO309_002903"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR5847978.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8788..9609
                     /locus_tag="OO309_002904"
     CDS             8788..9609
                     /locus_tag="OO309_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR5847979.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            9657..10403
                     /locus_tag="OO309_002905"
     CDS             9657..10403
                     /locus_tag="OO309_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5847980.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAAEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10377..11342)
                     /locus_tag="OO309_002906"
     CDS             complement(10377..11342)
                     /locus_tag="OO309_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCR5847981.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11339..12342)
                     /locus_tag="OO309_002907"
                     /pseudo
     CDS             complement(11339..12342)
                     /locus_tag="OO309_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            12382..13434
                     /gene="fbaB"
                     /locus_tag="OO309_002908"
     CDS             12382..13434
                     /gene="fbaB"
                     /locus_tag="OO309_002908"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR5847982.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13742..14596
                     /gene="gatY"
                     /locus_tag="OO309_002909"
     CDS             13742..14596
                     /gene="gatY"
                     /locus_tag="OO309_002909"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR5847983.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14625..15887
                     /gene="gatZ"
                     /locus_tag="OO309_002910"
     CDS             14625..15887
                     /gene="gatZ"
                     /locus_tag="OO309_002910"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCR5847984.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            15897..16349
                     /gene="gatA"
                     /locus_tag="OO309_002911"
     CDS             15897..16349
                     /gene="gatA"
                     /locus_tag="OO309_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR5847985.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            16380..16664
                     /gene="gatB"
                     /locus_tag="OO309_002912"
     CDS             16380..16664
                     /gene="gatB"
                     /locus_tag="OO309_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR5847986.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            16668..18023
                     /locus_tag="OO309_002913"
     CDS             16668..18023
                     /locus_tag="OO309_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR5847987.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
BASE COUNT         4718 a         4109 c         4570 g         4652 t
ORIGIN      
        1 ataaatgatt tcgtcttttt tattgatatc aacaatacca tttacataaa tctacattaa
       61 ttaatagatg tgataaatat ttaatattct taagtatttg ttgtatttat attgcattgg
      121 ttaatttgtt ttaaatcaat aaaacaatat gtttacattg cggcagcttt tttgatgtga
      181 gaaatttgtt tccaagagat acagactctt aacaaccaaa aataaatttt attaaggaac
      241 atattatgaa acgttcaatt attgctgccg ctgtcttttc ttcttttttt atgagcgctg
      301 gagtatttgc tgcagacgtt gataccggaa cattaaccat taaaggaaat attgcagaat
      361 ctccgtgtaa attcgaagcg ggtggtgatt cagtaagtat taatatgccg actgtaccaa
      421 ccactgtctt tgaaggtaaa gctaaatatt ctacctatga tgatgcagtc ggtgtaacca
      481 gcagcatgtt aaaaattagc tgcccgaaag aagttgctgg tgtaaaactc tcgttgatca
      541 caaacgacaa aataaccggt aacgataagg cgatagccag tagcaacgat accgtaggtg
      601 ataacagcga tgtcctagat gtttctgcac cttttaacat tgagagttat aaaacagcgg
      661 aaggtcaata tgctattccg tttaaagcaa aatacctgaa actgacagat aactcagtgc
      721 aatcaggtga tgtgttatct tctctggtta tgcgtgtggc gcaggattaa taaataaaat
      781 tgcaagtttc tccaggtaat aaacatggcc gctatcccca tggcggcttt ttaatttaag
      841 aggcattaag atgaaaggat tattatcttt actcattttt tctatggtcc ttcctgcaca
      901 tgccggaatt gttatctacg ggacgcgcat tatttacccg gcagaaaata aagaagttat
      961 ggtgcagttg atgaaccagg gaaatcgttc ttcgctgctg caggcgtgga ttgatgatgg
     1021 cgatacgtcg ttaccaccag aaaaaattca ggttcctttc atgttaacgc caccagtggc
     1081 aaaaataggg gcaaattccg ggcagcaagt aaaaatcaaa attatgccga ataaactgcc
     1141 cactaataaa gaaagcattt tttatctgaa tgttctgtat attccaccaa atagtccaga
     1201 gcaagaaggc aagaatgcac tgaagtttgc gatgcaaaac agaattaagt tgttttaccg
     1261 gccagcgggt attgctccgg taaataaagc gacctttaaa aagttactgg taaatcgcag
     1321 tggcaatgga ttggtgataa aaaatgactc agctaattgg gtgacgattt cggatgtcaa
     1381 agctaataat gttaaagtca attatgaaac tattatgatt gcccccttag aaagtcagag
     1441 tgttaatatc aaaagtaata atgcaaataa ctggtatctg accattatca atgaccacgg
     1501 caactatatt agtgacaaaa tttaattgca ggagctgcct atgttgagaa tgaccccact
     1561 tgcatcagca atcgtagcgt tattgctcgg cattgaagct tatgcagctg aagaaacctt
     1621 tgatacccat tttatgatag gtggaatgaa agaccagcag gttgcaaata ttcgtcttga
     1681 tgataatcaa cccttaccgg ggcagtatga catcgatatt tatgtcaata agcaatggcg
     1741 cgggaaatat gagattattg ttaaagacaa cccgcaagaa acatgtttat caagagaagt
     1801 tatcaagcgg ttaggcatta atagcgataa cttcgccagc ggtaagcaat gtttaacatt
     1861 tgagcaactt gttcagggtg ggagctatag ctgggatatc ggggtttttc gtctcgattt
     1921 cagtgtcccg caggcttggg tggaagaact ggaaagtggc tatgttccac cggaaaactg
     1981 ggagcggggt attaatgcgt tttatacttc ttattatgtg agtcagtatt acagcgacta
     2041 taaagcgtcg ggtaacaaca agagtacata tgtacgtttt aacagcgggt taaatttact
     2101 ggagtggcaa ctgcattctg atgccagttt cagtaaaaca aataacaatc caggggtgtg
     2161 gaaaagcaat accctgtatc tggaacgtgg atttgcccaa tttctcggca cgcttcgcgt
     2221 gggtgatatg tacacatcaa gcgatatttt tgattctgtt cgcttcagcg gtgtgcggtt
     2281 gtttcgtgat atgcagatgt tgcctaactc gaaacaaaat tttacgccac gggtgcaggg
     2341 gattgctcag agtaacgcgc tggtaactat tgaacagaat ggttttgtgg tttatcagaa
     2401 agaggttcct cctggcccgt ttgcgattac agatttgcag ttggccggtg gtggagcaga
     2461 tcttgatgtt agcgtgaaag aggcggacgg ctcggtaacc acctatctgg tgccttatgc
     2521 agcggtgcca aatatgctgc aacccggcgt gtcgaaatat gattttgcgg cgggggcgag
     2581 caagcaaagt gattttgtcc aggcaggtta tcagtatggt tttaataact tattgacgct
     2641 gtatggcggc acgatggttg ctaataatta ctatgcgttt accctcggaa cgggttggaa
     2701 cacgcgcatt ggcgcaattt ccgtcgatgc cacgaagtcg catagtaaac aagacaacgg
     2761 cgatgtgttt gacgggcaaa gttatcaaat tgcctacaac aaatttgtga gccaaacgtc
     2821 gacgcgtttt ggtctggcgg cctggcgtta ttcgtcgcgt gattaccgga catttaacga
     2881 tcacgtttgg gcaaacaata aagataatta tcgccgtgat gaaaacgata tctatgacat
     2941 tgccgattat taccagaacg attttggccg taaaaatagc ttttctgcca atatgagcca
     3001 gtcattgcca gaaggctggg gttctgtgtc cttaagtacg ttatggcgag attactgggg
     3061 gcgtagcggc agcagtaagg attatcagtt gagttattcc aataactggc ggcggataag
     3121 ctataccctc gcggcaagcc aggcgtatgg cgagaatcat catgaagaga aacgttttaa
     3181 tatttttata tcaattccct gtgattgggg tgatgacgtt acgacgcctc gtcggcaaat
     3241 atatatgtct aactcaacga cgtttgatga tcagggtttt gcctcaaata atacgggatt
     3301 atcaggaaca gtagggagtc gggatcagtt caattatggt gtcaacctga gtcatcaaca
     3361 tcagggaaat gaaacgacag ctggggcgaa tttgacctgg aacgcgccgg ttgcgacagt
     3421 gaatggcagt tatagtcagt cgagtactta tcgacagact ggagccactg tttcaggggg
     3481 cattgtcgcc tggtcgggtg gcgttaatct ggcgaaccgt ctttccgaaa cgtttgctgt
     3541 gatgaatgcg ccaggaatta aagatgctta tgtcaatggg caaaaatatc gcacaacaaa
     3601 ccgtaatgga gtggtggtat acgacggaat gacaccttat cgggaaaatc acctgatgct
     3661 ggatgtgtcg caaagcgata gcgaagcaga attacgtggc aaccggaaaa ttgccgcccc
     3721 ttatcgcggc gcggttgtac tggttaattt tgataccgat cagcgcaagc cctggtttat
     3781 aaaagcgtta agagcggatg gtcaaccatt aacgtttggt tatgaagtca atgatatcca
     3841 tggtcataat attggcgttg tcggccaggg aagccagtta tttattcgca ccaatgaaat
     3901 accgccatcg gttaatgtag caattgataa gcaacaagga ctttcatgca caatcacctt
     3961 cggtaaagag attgatgaaa gtagaaatta tatttgccag taatagtcag gtggttctat
     4021 ggaaattcgc ataatgctat ttatattaat gatgatggtt atgcctgtga gctatgcggc
     4081 atgttatagt gagttatctg ttcagcacaa tttggttgtt caggggaatt ttgcacttac
     4141 tcaaacacaa atagcgacat atgagcataa ttttaatgat tcgtcatgcg tgagtacaaa
     4201 tactatcacc cctatgagcc catcggatat tattgttgga ctttataacg ataccattaa
     4261 attaaattta cattttgaat ggaccaataa aaacaacatc acgttgtcaa ataatcagag
     4321 cagtttcacc agtggttatt cggtaacggt gacacctgcg gccagtaatg caaaagtgaa
     4381 tatttctgcg gggggcggtg gttcagtgat gattaatggt gttgcgacat tatccagtgc
     4441 ttcatcatcg acacgcggga gtgccgcagt acaatttcta ctgtgtttat taggtggcaa
     4501 gtcatggggt gcatgtgtaa atagctacag aaatgcattg gcacaaaatg caggtgtcta
     4561 ttccttcaat ctgacattgt catacaaccc gataaccaca acctgcaaac aggacgattt
     4621 attaattact ttagacagta ttcccgtttt acaattacca gccacaggta acaaagcaac
     4681 aataaatagt aaaaaaaggg atattattct gcgttgtaaa aatttattag gtcaacaaaa
     4741 tcaaacatca cggaaaatgc aggtgtattt atcaagttct gacttgttaa ccaacagcaa
     4801 cacaatactg aaaggtgcgg aagataatgc gtaggattta ttcttgaaag taatggttcg
     4861 ccagtcacac ttttaaatat cactaacagc agtaaaggat acacaaattt aaaagaaatt
     4921 gcggcaaagt caaaacttac agatacaacg gtttcaattc cgataacagc cagttactac
     4981 gtctacgata caaacaaagt taaatctggc gcactggagg caaccgcatt aatcaacgtg
     5041 aaatacgact aataacaagc aaaacgagca agtggctaat aataaaaaat aacaaggtgt
     5101 ctgcatttcc ccctcatgat gagggggctt tttttagcga tgataaaaaa tctcaccgtc
     5161 gtaggcttta atgattttac cgtcggtgtc gctgatcaac acgtacgcgc cacccatata
     5221 ggtccagtgc gtcccggcat caggcgcggg caggttacgt aactgccact gcttaatgtt
     5281 gtactcatcg gtgcggtaca tctctgggac ggtatcacct ggtttgaaat gcttaaagtc
     5341 agcaatgaaa ctacttaact catacttaga aattcccgag gtattggtcg aacccgcggt
     5401 ggctggtgcg gcccaggcgg cactggtaac cagcaaaagc acacccagca acatcttatt
     5461 tttaatggtc ataaactctc cactttgggg atctgtaata tcagtcgcat atctttcctg
     5521 tcatacaata taactgacaa gcgatatttt gttagtgatc ccatgatttc gtaaaaagtg
     5581 taaatgaaag gatgccctaa aagcatcctt agcgagcaga gagtcagcgg aaatagctga
     5641 tttaagaatc atcctgggct gaagataaca tgcaagccta aaggattgag ttatcgcatt
     5701 atgcccatga agccatgtac gcccatatac acaccgacta agccaatcaa cagactggaa
     5761 aaataggggg cgcgtttagc gagagtgtta aatccgctcc agcgttttgc gacctgctga
     5821 acgctgattg ctgcgccaac gcctacggtg acaagcgtta acgccaggcc aatgctgaaa
     5881 ctgacgacca gtgttgcgcc cagtgtcagg gctttcaact gaatgcatat caacagcacg
     5941 gtaattgctg ccgggcaggg gataaagcca ccggttaagc caaataacaa aatttgccag
     6001 ttgatgacct ctctaccatc aaagcgtcgt ttaatgtcat tggcatgggc tcgtgcatgg
     6061 gcatcctgat actcgccatg ttcgtgatgg tgatgatgtc cttggtcgtg gtgatgttcg
     6121 tgatcgtgat gatggtgatg atgctcataa tcatgcccgt gcatattctc cagccagttg
     6181 cgttcgccgc gctaggtacg ccagaacatc cagaacgcgg tgccaataat gatcactgcg
     6241 gaaatcagct ggagccacgg ttctgctgat tgagcagtaa agcgcttact gatcaccatc
     6301 ccgccaaagg caatcaacca gaccaccgcg gtatgcgaaa tggttgctgc cagtccgagc
     6361 atcaccgcct gtttaatggt gcctttgatg gcgatgataa acgccgccat catcgttttt
     6421 gagtgccctg gttccaggcc atgaagcgca ccaagtaaga tggcgctggg gatgaagaac
     6481 caggcgtttc cttgctgaag aagagttgta aattcggtca tgataataat tcttagtatt
     6541 aattcggcaa tctgattcta ctccccccca gtacctgata ctaccccccc agtagattaa
     6601 tagtgctatg atttttcata ttcttgttaa ccaggtgttg ccatgtctca tacaatccgt
     6661 gataaacaga aactgaaagc gcgtgccagt aagattcagg gccaggtcgt ggcgctcaag
     6721 aaaatgctcg acgagccgca cgaatgcgct gcagttttac aacagattgc tgctatccgt
     6781 ggagcggtaa acggtctgat gcgggaagtg attaaaggtc atctgacgga acacatcgtt
     6841 caccaggggg atgagctaaa acgtgaagaa gatctggatg tcgttctgaa ggtgctggat
     6901 tcatatatca aataatttat taacgcgatt gtaaaactgc cgtttttcct cgtttacaac
     6961 gggggcgctg gacattacca tcctcctctg cgatttatca tcgcaaccaa acgactcggg
     7021 gtgcccttct gcgtgaaggc tgagaaatac ccgtatcacc tgatctggat aatgccagcg
     7081 tagggaagtc acggaccacc aggtcattgc ttcttcacgt tatggcagga gcaaactatg
     7141 caagtcgacc tgctgagttc agcgcaatct gcgcacgcgt tacacctttt tcaccaacat
     7201 tcccctcttg tgcactgcat gaccaatgat gtggtgcaaa cctttaccgc caataccttg
     7261 ctggcgctcg gtgcatcgcc agcgatggtt atcgaaaccg aagaggccag tcagtttgcg
     7321 gctatcgcca gtgccttgtt gattaacgtt ggcacactga cgcagccacg cgcacaggcg
     7381 atgcgtgctg ccgttgagca agcaaaaagc tctcaaacac gctggacgct tgatccagta
     7441 gcggttggtg cgctcgatta tcgccgccat ttttgtcatg aacttttatc ttttaaaccg
     7501 gcagcgatac gtggtaatgc ttcggaaatc atggcattag ctggcattgc taatggcgga
     7561 cggggagtgg ataccactga cgccgcagct aacgcgatac ccgctgcaca aacactggca
     7621 cgggaaactg gcgcaatcgt cgtggtcact ggcgaggtgg attatgttac cgatggacat
     7681 cgtatcattg gtattcacgg tggcgatccg ttaatgacca aagtggtagg aactggctgt
     7741 gcattatcgg cggttgtcgc tgcctgctgt gcgttaccag gcgatacgct ggaaaatgtc
     7801 gcatctgcct gtcactggat gaaacaagcc ggagaacgcg cagtcgccag aagcgagggg
     7861 ccaggcagtt ttgttccaca tttccttgat gcgctctggc aattgacgca ggaggtgcag
     7921 gcatgaaacg aattaacgct ctgacgattg ccggtactga tccgagtggt ggtgcgggga
     7981 ttcaggccga tcttaaaacc ttctcggcac ttggcgctta tggttgctca gttattactg
     8041 cactggtggc gcaaaatacc cgtggcgtac agtcggtgta tcgcattgag cctgattttg
     8101 tcgccgccca gctcgattcg gtgttcagcg atgtgcgaat cgataccact aaaatcggta
     8161 tgttggcgga aaccgatatt gttgaagcgg tggcagaacg gttgcaacgt tatcagatcc
     8221 aaaacgtggt actcgacacc gttatgctgg caaaaagcgg cgacccgctg ctttcacctt
     8281 cggcggttgc tacgctgcgc agtcgattat tgccacaggt ttcattaata acgccaaact
     8341 tgcccgaagc tgccgccttg ctcgacgcgc cacacgcgcg caccgaacag gaaatgctgg
     8401 aacaagggcg atcgctgttg gcgatgggct gtggcgcagt gctaatgaaa ggtggtcatc
     8461 tggatgatga gcaaagcccg gactggctgt ttacccgcga gggtgaacaa cggtttaccg
     8521 caccgcgcat tatgaccaaa aacacccacg gcactggttg tacactctct gcggcgttgg
     8581 ctgcactacg cccgcgccat acaaactggg ctgacaccgt acaggaggca aaaatctggc
     8641 tttcatcggc gttagcccag gccgacacgc tggaagttgg tcacggtatt ggtccggttc
     8701 accacttcca cgcctggtgg tgagcaccgt actatagctg ctttggttat tgtcagcctt
     8761 tggcactgac attcagggat gaaaccaatg cagctaagaa ttaccagtcg aaaaaaactc
     8821 accgctctat tatgcgctct tgggctaatt tccatcgttg caatttatcc gcgtcagacg
     8881 gtgaattttt tctattcgac agcagttcag attacagact acatccactt ctacggttat
     8941 cgcccggtta aatctttcgc tattcgcatt cctgccagtt acaccattca cggattagat
     9001 gtttcgcgct ggcaggagcg tatcgactgg cagcgggtgg caaaaatgcg cgacaacgat
     9061 atccgcttac agtttgcttt tattaaggct acggaaggcg aaaagctggt ggacccctat
     9121 ttttcgcgta actggcaatt aagccgcgaa aatggcctgc tgcgcggggc gtatcattat
     9181 ttctccccgt cggtatccgc ttcagttcag gcgcgattat ttctgcaaac ggtggatttc
     9241 tcacaaggcg atttccctgc cgtgctggac gtagaagaac ggggaaaatt atcggcaaaa
     9301 gaattacgca agcgggtaag tcagtggcta aaaatggtcg aaaaacgcac gggaaaaaag
     9361 ccgattattt actcaggagc cgttttttat cacaccaatc tggcgggcta tttcaatgaa
     9421 tatccgtggt gggtggctca ctattatcaa cgtcgtccgg acaatgacgg catggcctgg
     9481 cgcttctggc agcattccga ccgtggacag gtagatggca tcaatggtcc ggtggatttt
     9541 aatgtgttta atggcacggg gatgagttgc aggcattcgt tgatgggatt aaagaaacgc
     9601 cttaaataag aagcagtgat atactcccac cagactccct ggcaggaaaa agacaaatgg
     9661 aacaagcgca tacccagctg attgcccaac tgaacgagcg tattttagcg gcggataaca
     9721 cgccgcttta tattaagttt gccgaaacgg taaaaaatgc cgtgcgcagc ggggtgctgg
     9781 agcatggcaa tattttgccc ggtgagcgtg atttaagtca gttaaccggc gtttcgcgca
     9841 ttacggtgcg caaggcgatg caggcgctgg aagaagaggg cgtggtgacg cgttcgcgcg
     9901 gttacggcac gcagatcaac aacatcttcg aatactcgtt gaaagaagcg agaggttttt
     9961 ctcagcaggt ggtattgcgc gggaaaaagc ccgatacgct atgggttaat aagcgtgtcg
    10021 tgaaatgtcc cgaagaagtt gcgcagcagc tggcggccga agcgggaagt gatgtctttt
    10081 tgcttaagcg tattcgctat gtcgatgaag aagcggtatc gattgaggaa tcgtgggttc
    10141 cggcgcattt aattcatgat gttgatgcca tcggaatttc tctttatgac tatttccgca
    10201 gccagcacat ttacccacaa cgtacgcgtt cccgcgttag cgcccggatg ccggatgccg
    10261 agtttcagtc acatattcag ttagatagca aaataccggt gctggtgatc aagcaagtgg
    10321 cgcttgacca acagcaacgg cctattgagt acagcattag ccactgtcgc agcgatctat
    10381 acgtttttgt gtgcgaggag tagttcctcg cgcgttggcg cacaatcacc gccccgatgc
    10441 ccgacaaccc acgacgccac tgcattgccc agtaatacgg catccgccag tggcagacca
    10501 gaggccagcc cggcaagtac gccacctgca tgactgtcgc ccgccccaat ggtgtctaca
    10561 acttgcgttg gaaatgccgg aatgcagcca gaagcgtcgt tgctgaaata ccatgcgcct
    10621 tctttatcga gacgaacgat caacggcgcg gcaaatttct cctgccattg cttgccaagt
    10681 gttgttatct ctgcggataa agcaaaacgt tcggcggcaa tctcagcctc ttgacgattg
    10741 agtgacacta aaggtcgaca ggccatgatc cgcgccagta atgcgtccgg gatatcgcca
    10801 atacgtgggc aaaaatcgat aaacggcgtc acgtcttgca gcttttccag ccattccact
    10861 aacaattcgc cgcagggcga ggccagttga taaccggaaa aatagagcag gttgccaggc
    10921 gcaacggtta atcgtgccaa ccattggcga ttccattgat tttccacacc gctgaatgac
    10981 ataaaagtgc gttcgccatc cggctcaacc agtgccagac accagccgtt atcgctttcg
    11041 gcgttatcga tcaggctgat taacccctct ttcgccatcc ggttgcgaat aatctccgcc
    11101 cacacgccct gaccgagcgg caaggcatta cccgcttcga tgccgaggcg ctttaacgcc
    11161 acggcaatat tcagtgcgca gccgccaacg ttaacgctct gctgtttcag ttcgatatcg
    11221 cacccacgcc aggggagggc ataagcgtcg gcgatcacgt caatgaccgc cgcgccgacg
    11281 accatcaccg gctgacgcgt ggttaattca ggcagcagtg tgtgtaatcg agcgccgctc
    11341 ataccgcctc ccgttgttga cgatatttcg ccagcgctgt ggcatagcgg ttgaaatcaa
    11401 gctgatttac cgcatccagc tccgccttta acgcaggatc gacagcgtta atgccatgca
    11461 acgcgccgca aatcgccgtc gccatagcac ctatggtgtc tgtgtcgccg ccaagattgg
    11521 cgcacaggac ggcgcagcgg tttgggtcgg tttgtgccag ttcaactagc gcaatggcgc
    11581 acggaacgga ctcaatagtg ctggtgcctg cgccaacgac ctggtaaagc tgttcgctgg
    11641 cggattcggt gccgtcggca ttacgcacaa ttttcagcgc aatctccaga cgtgccgcca
    11701 gtgaggcgct gaacgtggtg gtgcgttttt gctgtgcatg tcgcgcaatt gaaggcaatg
    11761 aatcaacaat cgctgaccag cgttctccgt caatggcacg agaaatcgcc catgcgatga
    11821 ctaccgcgcc tgcaaccgcc agatcggatt tatgggtcgg gctggacgcc agcgccacat
    11881 catcaataaa ggaatcaaca tcacgcgctg gaagcaaaca acctaatggc gagacgcgca
    11941 tcgctgcgcc gttggttacg ccgttgtttt ccagttcagc aacgggttta ccgtcgcgaa
    12001 tggcgttaag cgcaatcttc gaggtcggac ctagtacgtt tttgttaaag gcgtcgaaac
    12061 gcaacgccca gtcgagaata ttacgcccaa tcagattcgg atcgatcttg ccttcacatt
    12121 ccagtaacgc atccgccaga cacaggccat cgaggtatcg tcggtgaatt cggcgcggtt
    12181 aaaataacag gccgcgttat tctcctttgg tccaggcaga aaacggtcaa tccagccaaa
    12241 gtgtgcttta acgcggctgc gtggccaaag ctcggagggc atccccatcg catcccctaa
    12301 cgcctgccca taaagagcac cgagaatacg ttctgttttc atttaacttc cccttgaatc
    12361 ggaatttacg ggagcatagt aatgacagat attgcgcagt tgcttggcaa agacgccgac
    12421 aaccttttac agcaccgttg tatgactatt ccttctgacc agctttatct ccccggacat
    12481 gactacgtag accgcgtgat gattgacaat aatcgcccgc cagcggtgtt acgtaatatg
    12541 cagacgttgt acaacaccgg gcgtctggct ggcacaggat atctttctat tctgccggtt
    12601 gaccagggcg ttgagcactc tgccggagct tcatttgctg ctaacccgct ctactttgac
    12661 ccgaaaaaca ttgttgaact ggcgatcgaa gcgggctgta actgtgtggc atcaacttac
    12721 ggcgtgttgg tgtcggtatc gcggcgctat gcgcatcgca ttccatttct cgtcaaactt
    12781 aatcacaacg agacgctaag ttacccgaat acctacgatc aaacgctgta tgccagcgtg
    12841 gagcaggcct tcaacatggg cgcggtggcg gttggtgcga ctatctattt tggctcggaa
    12901 gagtcacgtc gccagattga agaaatttct gcggcctttg aacgcgcgca cgagctgggc
    12961 atggtgacag tgctgtgggc ctatttgcga aactccgcct ttaagaaaga tggcgttgat
    13021 taccatgttt ccgccgacct gactggtcag gcgaaccatc tggcggcaac cataggtgca
    13081 gatatcgtca aacaaaaaat ggcggaaaat aacggcggct ataaagcgat taattacggt
    13141 tataccgacg atcgcgtgta cagcaagtta accagcgaaa acccgattga tctggtgcgt
    13201 tatcagttag ctaactgcta tatgggccgg gctgggttga taaactccgg cggtgctgca
    13261 ggcggtgaaa ctgacctcag cgatgcagtg cgtactgcgg ttatcaacaa acgcgcaggc
    13321 ggaatggggc tgattcttgg acgtaaggcg ttcaagaaat cgatggctga cggcgtgaaa
    13381 ctgattaatg ccgtgcagga cgtttatctc gatagcaaaa ttactattgc ctgatacgcc
    13441 tcatacgaac agcaccaccc cgtaggacgg ataagacgtt tacactgcat ccggcaacct
    13501 atgcctgatg cgacgcggaa gcgtcttatc atgcctacat agcactgcca cgtatgttta
    13561 caccgcatcc ggcataaaaa cacgcgcact ttgctacggc ttccctatcg ggaggccgtt
    13621 tttttgcctt tcactcctcg aacaattttc atattgttgt ttttgcgatc attatctcga
    13681 catttaaaag caaataattt cattatattt tgaaatcgaa agcaaacgac aagatatgaa
    13741 aatgtacgtg gtatcgacaa agcagatgct gaacaacgca cagcgcggcg gttatgcggt
    13801 tccggcattc aatattcaca atctcgaaac gatgcaagtg gtggtggaaa ccgctgccaa
    13861 cctgcatgcg ccggtcatca tcgccggaac gcctggcaca tttactcatg ctggtacaga
    13921 aaatctgttg gcgctggtca gcgcgatggc gaagcaatat caccatccac tggcaattca
    13981 tctcgaccat cacacgaaat ttgacgatat cgctcagaag gttcgttctg gcgtgcgctc
    14041 agtcatgatt gacgcctcgc atttgccttt tgcgcaaaat atttcacggg tcaaagaggt
    14101 ggtggatttt tgccatcgct ttgatgtcag cgtcgaggcg gagctggggc aacttggcgg
    14161 ccaggaagat gatgtgcaag tcaatgaagt cgatgcgttg tacaccaacc ccgcacaggc
    14221 gcgtgaattt gccgaggcaa ccgggattga ttccctggcg gtcgccatcg gcacggctca
    14281 cggtatgtat gccagcgcac cggtgcttga tttttctcga ctggagaaca ttcgccagtg
    14341 ggtgaactta ccgctggtgc tgcatggcgc gtcaggatta tcgactaagg atattcagca
    14401 aaccatcaaa ctggggatat gcaaaatcaa cgttgcaacg gagctgaaaa atgccttctt
    14461 gcagtcgtta aaaaattacc tgaccgagca ccccgaagcg accgatcccc gggattattt
    14521 gcagtcggct aaatccgcaa tgcgcgatgt ggtgagcaaa gtgattgccg attgtggctg
    14581 cgagggcagg gcataacgca cctgccattt aacaaggaaa aaacatgaaa acgttaattg
    14641 cccggcataa agctggtgaa catatcggca tatgttcagt ctgttctgcc catccgttgg
    14701 ttatcgaagc ggcgctggca tttgatcgca acagcacgcg caaagtgctg attgaagcaa
    14761 cgtcaaacca ggtcaatcaa tttggcggtt ataccggaat gacaccggca gactttcgcg
    14821 aatttgtttt tacgattgcc gataaagttg ggtttgcacg cgaacgcatt attctcggcg
    14881 gcgatcatct gggaccaaac tgctggcagc aagaaaatgc ggatgcggcg atggaaaaat
    14941 ccgtcgagct ggtaaaagca tatgttcgtg ctggcttcag taaaattcat cttgatgcgt
    15001 caatgtcctg cgcgggggat accataccgt tagcaccaga aacggttgcg gaacgagctg
    15061 ctgtgctttg ctttgctgcg gaaagtgtgg cgacagattg ccagcgtgag caactgagct
    15121 atgtcattgg cactgaagtt ccggttccgg gcggtgaggc cagcgccatt cagtcagtac
    15181 acatcaccca tgttgaagat gccgccaata ctttacgtac gcatcaaaag gcctttattg
    15241 cccgtgggct gacagaggcg ttaacacgtg tgattgccat cgtggtgcag ccgggtgtgg
    15301 aatttgatca cagcaatatt atccattatc agccgcagga agcgcaggcg ctggcgcaat
    15361 ggatagaaaa cacccgaatg gtttatgaag cacattctac cgattaccag acccggacgg
    15421 cttattggga attagtccgc gatcactttg caatattgaa agtcggtccc gcattaacct
    15481 ttgctttacg cgaggcgata tttgcactgg cacaaattga gcaggaactt atcgcccccg
    15541 aaaatcgcag cggttgcctg gcggtaattg aagaagtgat gctcgacgaa ccgcaatact
    15601 ggaaaaaata ttatcgcacg ggttttaacg attcattact ggatattcgt tacagcctgt
    15661 cggatcgtat tcgttattac tggccgcata gtcggattaa aaatagcgtt gaaacgatga
    15721 tggtgaatct gcaaggcgtg gacatcccac tgggcatgat tagtcagtat cttcccaaac
    15781 aatttgaacg cattcagtcc ggggaattat cagcaatgcc gcatcagctt attatgaata
    15841 aaatttatga tgttttgcgc gcctatcgct acggctgtgc ggaataagga cggtatatga
    15901 ctaacctgtt tgttcgtagc ggaatttctt ttgtcgatcg tagcgaagtt ttaacctata
    15961 tcggtaatga gatgctcgcg aaaggtgtgg ttcatgatac ctggccacag gcattaattg
    16021 ccagagaagc agaattccct accgggataa tgcttgagca gcacgccatt gcaataccgc
    16081 attgtgaggc gattcatgct aagtcgtcag ccatttatct gttaaggcca acaaataaag
    16141 ttcattttca gcaagcggat gatgataacg acgtggcggt atcgttggtt attgcgttga
    16201 ttgtggaaaa tccgcagcag caattgaaac ttttacgctg tttatttggc aagttacaac
    16261 agcccgatat cgtcgagaca ctaattactc ttcctgaaac tcagttaaag gaatacttca
    16321 caaagtatgt tttagattca gacgaataaa tccctctgta acaataaaaa ggactattta
    16381 tgaaacgcaa gattattgtc gcttgcggag gcgcggttgc gacctctacg atggcggcgg
    16441 aagaaattaa agagttgtgt cagagtcata atattcctgt tgaattaatc cagtgtcggg
    16501 ttaatgaaat agaaacctat atggatggtg tgcatttgat atgtaccact gccagggtgg
    16561 atcgtagttt tggcgatatt ccgttagttc acggcatgcc ttttgtttct ggtgtcggta
    16621 tcgaagcatt acaaaataaa attctgacta tcttacaggg atgacctatg ttttcagaag
    16681 tcatgcgtta tattctcgac ctcggcccta cagtgatgct gccggttgtc atcattattt
    16741 tttctaaaat attaggcatg aaggcaggcg attgctttaa agcgggtctg catatcggga
    16801 ttggctttgt tggcattggc cttgtgattg gcttaatgct ggattccatt ggtccggcgg
    16861 cgaaagcgat ggcggaaaat ttcgacctga atctgcatgt ggtcgatgtc ggctggccgg
    16921 gctcttcacc aatgacctgg gcgtcgcaaa ttgcgctggt ggcgattccg attgcgatat
    16981 tggttaacgt ggcgatgtta ctgacccgta tgacgcgggt ggtaaatgtt gatatctgga
    17041 atatctggca tatgaccttc accggcgcgt tgctgcacct ggcaaccggt tcatggatga
    17101 tagggatggc gggtgtggta attcacgcgg cgtttgttta taagctcggc gactggtttg
    17161 cccgcgatac ccgaaatttc tttgagctgg aaggtattgc tattccgcac ggtacgtcgg
    17221 cgtatatggg gccgattgcg gtgctggtcg atgctatcat cgagaaaatc ccaggcgtta
    17281 accgaattaa atttagcgcc gacgatattc agcgcaaatt tggccctttt ggcgaacctg
    17341 tcaccgtggg ttttgtgatg gggctgatta tcggcatcct cgcgggttac gatgtcaaag
    17401 gtgtattgca gctggcggta aaaacggcgg cagttatgct gctgatgcca cggatgatta
    17461 aacccatcat ggatggttta acgcccatcg ctaagcaggc ccgtagtcgt ttacaggcga
    17521 agttcggcgg tcaggagttc ctgattggcc tggatcccgc attactgctg gggcatacgg
    17581 cggtggtatc ggcaagcctg atttttattc cgctcaccat tttaattgct gtttgtgttc
    17641 cgggtaatca ggtactgccg tttggcgatc ttgccactat cggcttcttc gtggcgatgg
    17701 cggtcgcggt gcatcgtgga aatctgttcc gcaccttaat ctcgggtgtc atcattatga
    17761 gcatcaccct gtggatcgcg acgcaaacta ttggtttgca cacccaaccg gcggctaatg
    17821 ctggggcgtt aaaagccggg ggtatggtgg cttcaatgga tcagggcggt tctcccatta
    17881 cctggttact gattcaggtt ttctccccgc aaaatattcc cggtttcatt attatcggcg
    17941 caatttatct gaccggtatt ttcatgacct ggcgtagagc gcgtggtttt actaaacaag
    18001 agaaagccgt tctcgcagaa taatttttac ctgaggggga ggttatccc
//
LOCUS       DAJTQQ010000079        17535 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000079 DAJTQQ010000000
VERSION     DAJTQQ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.079"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(60..572)
                     /gene="chbR"
                     /locus_tag="OO309_002914"
                     /pseudo
     CDS             complement(60..572)
                     /gene="chbR"
                     /locus_tag="OO309_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(580..930)
                     /gene="chbA"
                     /locus_tag="OO309_002915"
     CDS             complement(580..930)
                     /gene="chbA"
                     /locus_tag="OO309_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR5847988.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(989..2337)
                     /gene="chbC"
                     /locus_tag="OO309_002916"
                     /pseudo
     CDS             complement(989..2337)
                     /gene="chbC"
                     /locus_tag="OO309_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2422..2742)
                     /gene="chbB"
                     /locus_tag="OO309_002917"
     CDS             complement(2422..2742)
                     /gene="chbB"
                     /locus_tag="OO309_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR5847989.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3041..3379)
                     /gene="osmE"
                     /locus_tag="OO309_002918"
     CDS             complement(3041..3379)
                     /gene="osmE"
                     /locus_tag="OO309_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR5847990.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3581..4408
                     /gene="nadE"
                     /locus_tag="OO309_002919"
     CDS             3581..4408
                     /gene="nadE"
                     /locus_tag="OO309_002919"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR5847991.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4638..5525
                     /gene="cho"
                     /locus_tag="OO309_002920"
     CDS             4638..5525
                     /gene="cho"
                     /locus_tag="OO309_002920"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR5847992.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5485..6060)
                     /gene="ves"
                     /locus_tag="OO309_002921"
     CDS             complement(5485..6060)
                     /gene="ves"
                     /locus_tag="OO309_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR5847993.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6263..6748)
                     /gene="spy"
                     /locus_tag="OO309_002922"
     CDS             complement(6263..6748)
                     /gene="spy"
                     /locus_tag="OO309_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR5847994.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7078..8046)
                     /gene="astE"
                     /locus_tag="OO309_002923"
     CDS             complement(7078..8046)
                     /gene="astE"
                     /locus_tag="OO309_002923"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR5847995.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(8039..9372)
                     /gene="astB"
                     /locus_tag="OO309_002924"
                     /pseudo
     CDS             complement(8039..9372)
                     /gene="astB"
                     /locus_tag="OO309_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9369..10847)
                     /gene="astD"
                     /locus_tag="OO309_002925"
     CDS             complement(9369..10847)
                     /gene="astD"
                     /locus_tag="OO309_002925"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCR5847996.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10844..11878)
                     /gene="astA"
                     /locus_tag="OO309_002926"
     CDS             complement(10844..11878)
                     /gene="astA"
                     /locus_tag="OO309_002926"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR5847997.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11875..13095)
                     /gene="astC"
                     /locus_tag="OO309_002927"
                     /pseudo
     CDS             complement(11875..13095)
                     /gene="astC"
                     /locus_tag="OO309_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13541..14347
                     /gene="xthA"
                     /locus_tag="OO309_002928"
     CDS             13541..14347
                     /gene="xthA"
                     /locus_tag="OO309_002928"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR5847998.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14514..15224
                     /locus_tag="OO309_002929"
     CDS             14514..15224
                     /locus_tag="OO309_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5847999.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            15229..15906
                     /gene="ydjY"
                     /locus_tag="OO309_002930"
     CDS             15229..15906
                     /gene="ydjY"
                     /locus_tag="OO309_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR5848000.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            15921..16628
                     /locus_tag="OO309_002931"
     CDS             15921..16628
                     /locus_tag="OO309_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5848001.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16628..17176
                     /locus_tag="OO309_002932"
     CDS             16628..17176
                     /locus_tag="OO309_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR5848002.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            17186..17512
                     /locus_tag="OO309_002933"
                     /pseudo
     CDS             17186..17512
                     /locus_tag="OO309_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         4340 a         4661 c         4287 g         4247 t
ORIGIN      
        1 gccgattcac taaactgctc tttatcatgc atcttttcta ccgtactttt cagccactac
       61 ggtacatcat caatcacctg ttcttcgcgg taatgacgta aacggttaat gacataaaaa
      121 gtaaccatct caacaaactc ttccagccct gtttcgcgga aatttaatga agaaatcact
      181 gtttccacat aggtgagaaa agcgttattg gtccggtata cctgcgaagc aacaaagcaa
      241 taaggcaaca acggcaggta atgctgctca aaaaagcgtt tactgatccc aacgttcaat
      301 atgcgcgtgg caccaaactc ataaaaactt tgatggtgcg aacctaacgg aataaaaaca
      361 aaatcgcccc gttccagtaa cacgcgctta ccgttaatct cctggaaata acgcccggtt
      421 aataccagag taaattcata atagtcgtgc tgatgcagtc cgctgatact ctcagttttg
      481 ttatagataa acacatggaa gtttttgcca ttaaacaact gctgttctcg ggcagtggca
      541 atttccggcg cgttaatcac tggctgcatc atcgactcct tatgccttca gtttttcatg
      601 aagctcaatt aattcagtaa tcagttcacg cgcaagcatg gacgtcatta aatgatcctg
      661 agcgtggacc agcaccagac tcactttcat cttaccttcg cccgcatcgc cttcaatcag
      721 tttcgtctgt accagatgcg cttcattcaa tgccatccgt gactgatcca tcatggcttt
      781 cgctgcggca aaatcgccct gcttcgcctg tttcaatgcg gcatacgcca ggctacgcgc
      841 ttgtccagag ttgatgataa gccccatcac cacttcttcc agctcttcag cttctgtttg
      901 cgtatcagga atattatcga gatccatcat acatcgttcc tctctcttct taccggcaag
      961 attacccgta ccggcatcga ttaaaatttc aggggttagc gatatcttct tcgctctctt
     1021 ctttatcaat cgcattttgt gctttgttag ccaccacaac aaagggcaga taaattaacg
     1081 ttgcgatgcc aaggttgaag agtgcgacca gcaatgcggc gacgctaccg ttggtgttaa
     1141 agaaggctcc cagaccggtt ggcatggtcc acggtgcaat attggtcacc ggaggaatga
     1201 tgcccatgta gtacgctgcg agggtgattg ccgccagaat cggttgtacc agtacaaacg
     1261 ggataaacat caccgggttc atgataattg gcagaccaaa cagaatcggt tcgttaatct
     1321 ggaagatgcc ggacggcagc gccagttttg ccacctgacg ataatcagca cggcgagagg
     1381 cgataaagat agccaggatc aggcctaaag tcgcaccact gcccccaagg aaaataaagg
     1441 agtccagcat cggcttggcc cagatatgga aggtcttacc ggctgccagc gccgcttcga
     1501 cggaaccata ttgctgatag gtcgcgatat tttccagcgc ccacggcgtc ataatgccgt
     1561 tgtccagtgc ggtcagcgcc agcgcgccat gaataccgaa gaaccagagc agtggaacaa
     1621 agatcacata ggcccagccc accacgctac ccaacgatgc cagtggggtt gagatggtat
     1681 ccataatgat ctgatggaag ttggtgcccc aggtattcaa cgcccaggca ataatcccca
     1741 tcacggaaag aataataaag ccgggaatta atgccgagaa agaacgcgat actgaagcag
     1801 gtacgctgtc gggcagttta atgacccaat tgcggcggac aataaaggta aacatttctg
     1861 ccaccaccag gccaataata atcccggaga tgatattcgc cccacctaac cagtttgcgc
     1921 caaccgcata ggcctcaccg acactatatg gggtgacggt cataaatgct gcaacggata
     1981 acaacccagc cgccagcgca tcgactttac gctcttctgc cagcgccatg ccaataaaga
     2041 acggtgccat taaagacatt attcctaatg ttccgttata tacgttgccg ccaatacctt
     2101 tcagaccatt aagtgtttca atggttgagg cgtcgaggcg aatacctaag gaataaaaaa
     2161 cgacccctcc ccaaagctta gaaaaacgtt gttaattaat acaaacatgg cccccgcaag
     2221 ggttaacggc attaagcgaa taaagccatt tttgattgca ttaacgtgtg gctgctttcc
     2281 tattttaact gcaaaaggga ggagtacctt ttcaagcgat gcaataacat tactcataga
     2341 aaaataccct taaaaaccgc aatttaaata ttacggtatt gatttatgaa ataactcttt
     2401 gacgggaaaa tttaaaataa attaatttgc tgcggctttt ttaatcgctg caaccgcagc
     2461 cttaagcacg cctaaaccat cgactttgcc ataaagcagc gagtcaatta cttcaaccgg
     2521 tttgttgggt aacaaacgct ggatttcggg caacatataa gcaatctgcg gccctaataa
     2581 cacgacatcg gcattctgac ctttttcacc agccagtgtt tccggaaatg cttcaataat
     2641 gaccggaact tcatattttt ctgcctgtgc gcgcattttt gataccagta aagagataga
     2701 catgcccgca gaacaaaaca gataaatgtg tttcttttcc ataaaactgc cctcatcgac
     2761 gattatctgt cagccagaca ctccgcaagc cttgacctgc ttccatgctc tgggtaactt
     2821 gcgaaaccaa catgactttt tttgtggctg aaaggagtat acggtataga cctgagcaat
     2881 agtatttctt tgaccttata aattgtttct ccttgacctg tggttatgac cctcttcaca
     2941 tttcgggcaa ataattcgcg aagataatta agaactcagg ccaaaaaaaa ccggcgcaat
     3001 ggccggtttc cgttgttact caagctttca gacgaattga ttacttcgca gcctgtggat
     3061 cagtgtcgta ttcagcacag gtctgataac cggagttgat gacatgtccg gtatcatcta
     3121 acgcgacaaa gtaggtttct gctttaccat cacgttgacc caggatgtag gtctggcaag
     3181 taccgcgagc atggatcatg ctcacttcag acgaaggttt acccgcaatt tgtgcaacct
     3241 gcgcccggct catgcctttt ttgacgtctt tcaccacagg ctgtacaaac tggtctttgg
     3301 tacgatcata agctgtacaa cccgccagca tggttaatac cgccgctgca ctcagaattc
     3361 ctgccatatt cttgttcata ttccgtcctc ttgtttatca gcgtgttaga taagcctgga
     3421 atacattggg cgctttttca agcccgtgaa cgaaacggct ccgctttcag aggattcctg
     3481 tatgacgttt taaccaccat tcagcccgct gtcgcttgtc gtttcagtag caacgggtta
     3541 gctttaagga agttttgtct tttctgtctg gaggggttca atgacattgc aacaacaaat
     3601 aataaaggcg ctgggcgcaa aaccgcagat taatgctgaa gaggaaattc gtcgtagtgt
     3661 cgattttctg aaaagctacc tgcaaactta tccgttcatt aaatcactgg tgctcgggat
     3721 cagcggcggt caggactcca cgcttgccgg aaagctgtgc cagatggcga ttaatgagct
     3781 gcgccaggaa actcgcaacg aatcactgca atttattgcc gtacgcctgc cctatggtgt
     3841 tcaggctgac gaacaagatt gccaggatgc cattgccttt attcaaccgg atcgcgtatt
     3901 aaccgttaat atcaagggcg cggtattggc tagcgagcag gcattgcggg aagcaggcat
     3961 tgaactgagc gattttgtcc gtggcaatga aaaagcgcgt gagcggatga aagcacaata
     4021 tagcattgcg ggtatgacca gcggtgtcgt ggtgggcacc gatcatgcag cagaagccat
     4081 taccggattc ttcactaaat atggtgacgg cggtacggat attaatccgc tgtatcgtct
     4141 caacaaacgc cagggtaaac agttactgac ggcattaggt tgcccggaac acctttataa
     4201 gaaagcccca actgccgatc ttgaagatga tcgcccttct ctgccagatg aagtggcact
     4261 cggcgtgacc tatgacaata tcgacgacta tctggaaggg aaaaacctac ctgaacaggt
     4321 cgccagaaca atagagaact ggtatctgaa aaccgaacat aaacgccgtc cgccaattaa
     4381 cgttttcgat gatttctgga aaaagtaata atttgcacct attggattgt gcgaaaaaga
     4441 gtaatttgtt cacgccggat gcggcgtgaa cgccttattc tacctacaaa actatgcaaa
     4501 ttcaatacat tgcaggagtc gaataggcct gacaggcgta gcacgtcaga cggtgtaacc
     4561 tttgtcatca acccgcctct tttttaatcg cctcccgcct gttacactgg atagataacc
     4621 agcattcgga gtcaacagtg gtacggcgtt taacttctcc gcggctcgaa tttgaagctg
     4681 cggcaattta tgaatatccc gaacatttac attcattcct taatgactta cccacccgac
     4741 caggggtgta tctgtttcat ggcgaaagtg acaccatgcc gctctatatc ggcaaaagcg
     4801 ttaatatccg cagtcgggtg ctttctcatt tacgcacccc ggatgaagcc gccatgctac
     4861 ggcaatcccg gcgaattagc tggatctgca ccgcaggcga aatcggcgct ctgctccttg
     4921 aagcgcgatt aatcaaagaa caacagccgc tgtttaataa acggttgcgc cgcaatcgcc
     4981 agctctgtgc cctgcaatta aatgaaaagc gcgtcgatgt ggtgtatgcc aaagaggtgg
     5041 atttttcacg agcccccaac ctgtttggcc tgtttgccaa taggcgcgca gctttgcaag
     5101 cattgcagag catcgctgat gaacaaaaac tttgttatgg cctgttggga ctggaaccgt
     5161 taagtcgcgg tcgtgcatgt tttcgttcag cgctaaaacg ttgcgccgga gcatgctgcg
     5221 gtaaagagag ccatgaggaa catgcgctac gcttgcgcca gtctctggag cgtttgcgag
     5281 tggtgtgttg gccttggcaa ggtgcggtgg cactgaaaga acagcacccg gaaatgactc
     5341 aatatcatat tattcaaaac tggctgtggc tgggggcggt taattcgctg gaagaagcga
     5401 caacgttaat tcggacaccc gccgggtttg atcacgacgg ttataaaatt ctttgtaagc
     5461 cgctgctttc cggtaactat gaaattactg aacttgatcc ggcgaatgac cagcgagcca
     5521 gttgatttca ctgaacaaca gtttaccttg cggttgtaaa agccgcaggg tgtgcctgcc
     5581 atcgaaccag cacacgccct ggtcggtagt gagcagtttg tcgcccagtt gccacgcccc
     5641 gttaatcaca aacaccaccc cgccgcgtga gccaaaggta gtaaacgtac gttcggcaat
     5701 tctgactttc gccttacaga catccaggcg cgtcataata ttgaaatcca tcgacatctg
     5761 ccctgccgtc agtttcgctt ttaccacctg gtccgccgta aaggcaaaag gctgtaacgg
     5821 ttttaaggta tggttaaagc ggtctgcgct ttcaaggagc atctcgccgc cttccagcaa
     5881 cgtcactatc ctttccatgc cgggaaacaa agaaaactcc ccattcgccg cgatggatgc
     5941 aatgctggca cgccagtaaa aatcacgttt tgccggtggg aaagtgcaaa tttcgcgcgt
     6001 ttcaccggca gcgtttcgcc acagattcac cgacatttta cgcatatcaa agtattccat
     6061 actcgctccc ggcccgttct tttcattgtt atcgttcagg taaccgtgga tggttatcgg
     6121 caactcgggt gatttacgtt agtggtgatc aggctttttt tacaactgtt tgagaagaga
     6181 aaagaaaacc gccgatcctg tccaccgcat tactgcaagg tagtggacaa gaccggcggt
     6241 cttaagtttt ttggctgaaa gattattcag cagttgcagg cattttacct tttgccgctg
     6301 gacgttctgt cagacgcttc tcaaaattag cattaaattg ctttttctgt tccggcgtca
     6361 ggatgttgta aattttgttc tgggtttcca tgtgcgccag catgttagct ttgcgctgtt
     6421 cttccatttt tgcgatctgc gcttcagctt ttgctttatc gaaggtatcg ctggcaatga
     6481 tgtcatgcat tgcgcggcgt tcttccagcg gcggacgttt catctggtca cgctggcctt
     6541 tcatgatttc gtggatctgc tgtttctgcg cgtcggtcag gttcaggtct ttgaacatca
     6601 tgtcctgatg cggaccgaac ttgcctttgt ggtgcatcat cggcttcgcg tcagccggtg
     6661 ctgcggtagt ggtgtctgcg gcatgggcca ggttagccgc gccaagagcc agggtagagg
     6721 caacaaacag tgcagttaat ttacgcatat tctatatcct tcctttcagt tatttattac
     6781 ggctttctta agtagcgtgc cgtgttgacg agattaactt tactgacttt agcgtcaatt
     6841 aatcagagca acggtaaaac aatgaaagtg taaaaaacac ttttgcgcca attatggaga
     6901 aaaaaagaaa atttgatgga gagtgatgag agaatattac aacacgatga ttttgcagag
     6961 attatgaaga actataccgg atgactggtg ataaataaag caaataacca ggattaatct
     7021 gtattaattt ataagaaagc aacttaatac ccgcagaatg atttctgcgg gtaagtatta
     7081 gcttattttt tcgagcatta atcccgcgcg taatcccaac gctaccaacg gattagggaa
     7141 taacacatac tctacatcat gggttacggt aaaacgttcc tctccgtcct gcgccagcaa
     7201 tgttcctttc tcaaacggca taaaattcag cgtgtcactt gccatatgca tttcgaagga
     7261 cggcgagtgg cgagtaattt gcgaaaccac ccgataacgg agcggcggtg ttctcacgat
     7321 accgacactc tcaccagata gcagcgcagc aattgcgctg gcagttactg caaactggcg
     7381 aagatcgttt tgcccaaagg gcaacgcttt gccaagttcc aacgtacagg ccagcgcgcc
     7441 aaaatgtctg acgctgaaat gggtaaacgt accaccaggc tcctgatgga acaccagcgc
     7501 ctccagcccc gccgcaccca gccacgtcag aaatttctcg tcccagggaa tgtcgcgttg
     7561 cggtaataca ccgaactgcg gatgcaagga gccacgaatt gcggtatgta gatcaaggtg
     7621 ccaacgcaca gattctttgc cctggtcata aaaatcttcc aggcactgtt ccagttcgcg
     7681 cgcccgacag gtttctccgc tttcagcaaa tagctgccaa cgaccgccaa acattcggtt
     7741 catatcgcta tggcaataac gtttcccttg cttcagcgca ggaggattcc cgaggatcac
     7801 cagcaaccgc caacgtaacg ggatctcgcc gtgagatatc gcgccaagca acgcgtccag
     7861 catttccaca ggtgccgtct cattaccgtg gatccccgca gaaatcacca gcgcgccttg
     7921 cggtggcgtt aatggcgtca gttccagcac accatcgccc agccagcgcc agcgaacgcc
     7981 gttgatttct cgctcggtga taaccggttt tttacccgtt aaggtcagag caagaaaatt
     8041 atccattgcc ccctccctcg cgctggaacg gataaaccga accgagattc agtaattggc
     8101 tcaatacatc cagtgcttcc cgcccttcgc gcagcaattg cgggtcggcc agatcggcag
     8161 cagtaagacg atcgcggtag taacgatcca cccagtcatt gagcgcatta aacaaggtat
     8221 cgttcatcat caccgccgga ttcaccgccc ggcgttcttc ttctgtcaat accacccgca
     8281 accgcaggca cgccgggccg ccgccattcg ccatgctttc acgtaagtca aagactttta
     8341 gttcgctaat cgggttgtca gcggcaagga gttcattgag ataaccccat actccggcgt
     8401 gttcccgaca ctcctgaggc agcaccaaca tcatggaacc atcatcgcgg ctcaacagtt
     8461 ggctgttaaa cagataggta gacaccgcat ccgacacgga aacctgagtt gccggaactt
     8521 ctatcgccat aaaaccgttc acccgcgtgc cagtaactgt gactggcgag cgaacgcctg
     8581 ttggtggcaa aacagcacct ggcggttact cacggcaatc acgtcattat gaaaaacgct
     8641 ctggtcgata acgtccgggt tttgctgggc gaaaatcacc tgttggggat tcacctgatt
     8701 aagccttgcc accgcctcgc tggcttcgcg agtctgtcgc gccggataac gggaaggccg
     8761 ggtatcattg ccttcttctc gcccgtagac aaaaagtagc atacccggtt caccgtaatg
     8821 accgccgaga cgattgtggt ttgccgcccc ctcatcaccg agcaacgcta cctgcggcaa
     8881 cgccgaatgg acgctaaatt tctcttcgtc cttaaaaatc gcttttaaca gcgattcagt
     8941 gacgggcgct tccagcgaac ggtgaaattt attgttcagg ttggcaacgg tgagatgcac
     9001 tttgccatcc agcgtatcgg cagatggcgc gatcgttgcc gcattggcta cccacattgg
     9061 cgaagcggaa ctgacgctgg aaagccagtg cggtgcctgg cgtgcaactt tttccagtac
     9121 ctgctcatcg ctaccactga atcccaactg acgcagcacc ggaataaacg gacgctcgtg
     9181 cggcggaatc acggcctggg ggaatcccgc atcggcaagg gctttcattt tcagtaagcc
     9241 ctgttttgcc gccagtcgcg ggttagacac ctgaaaacgg tgacgggtag aggcttcatt
     9301 accaaacgac aggcccgcgt aatgatgcgt cagccctacc agcccgtcga aattaacttc
     9361 ccaggcgttc atcgcaccac ctcatcggaa aaatccagcc cggggttaag cgtggcgggc
     9421 aatgttaacg agtccgactc caggctcgcc ataggccatg cgcagtaatc tgcggcatac
     9481 caggcgctgg ggcgatggtt accggaagca ccaatgccgc cgaatggggc ggtactggca
     9541 gcaccagtaa gcggtttgtt ccagttaaca atccccgccc gcgcctccag caacagttga
     9601 tcgaattttt cccgctcggg ggaaaccaga ccgcaaaaga gtccgaagcg agtgttgttc
     9661 gccatccgaa tcgcctcatc aaaagtatca taacgccaga cgcgcaaaaa cggcccgaac
     9721 acctcttcat ccggcacacc gccaacgccc gtcatttcaa tgatccctgg tgtcagcaac
     9781 gatgtccctg cttgtaataa gcgcggcgca agcagggttc gtccgcccat cgcttccagt
     9841 tgctgccagg cagtaaccac ctgctgtgcg gcctgttcag aaatcagccc gccaataaac
     9901 ggctgcggtt cgtcatccca gttgcccggc gttaatcgct ggctgacggc aaccagacta
     9961 gcaagaaacg catcgccctg cgctccgctt ttgagcaata aacggcgggc gcaggtgcag
    10021 cgttgcccgg ctgtgacaaa cgccgactga atggtcagat ggacagccgc gtcgatatcc
    10081 gccacctcat cgataattag cggattatta ccgcccatct caagggcgag aattttctcc
    10141 ggctgaccgg agagctggcg atgcagctgg tagccggtat tggcgctacc ggtaaacagc
    10201 aaaccgtcga gatcctccag cgcactcagc gcctgacccg tttcacgccc gccctgcacc
    10261 aggttcagca cgcccggcgg caagccagcc tgctgccata aacgcattac cgcttcgcca
    10321 ctccacggtg tcagttcgct gggtttaaag ataatggtgt tacctgccag caatgccgga
    10381 acgatatgtc cgttcggcaa atgaccaggg aaattatacg gcccaaacac cgccagcacg
    10441 ccgtgcgggc gatgtcgcag gctcgccgca ccgtccggca tttcactacg ctgctcgccg
    10501 gtacgaacgt gatacgcctt aattgatatc gcgattttat tgatcatcgc cgtcacttcg
    10561 gttgccgctt cccagcgcgg cttacccgtt tctctggcaa taatcgcggt taattcggct
    10621 ttattgcttt ccagcaatcc ggcaaagcgt tcgacaacga cctgacgttc agccaatgag
    10681 agccgcgccc agcgcggaaa cgccgcacgg gctgcccgac aagcctgttc gacctgagcg
    10741 gcatcggcat cattgccttg ccataacacc tcgcccgata ccggattacg cttcacacgc
    10801 gatgcgccct ggcccgttat ccagtcaccg ttaatccata aagtcatgct gttttctcct
    10861 ctgcgcacag gcgcaccaga cgaacgcgat cccctgcgtg gcatttgagg gcatccagtt
    10921 gtgcggcggt taaaatcaaa cgctcggttg ccggatcggt acgcgccagc accacgcgga
    10981 aatggtgata attttcattg gcgaccaggc aggccggaaa atcgccctgc gcaggctgcc
    11041 cttctgccac ttccaccagc cgacttttac ggatggcgcg cacgcggtcg atgtcacact
    11101 caagcgttgg cccgccgtcg aagatgtcga tatagttgcg gtaacgaaaa ccttctttct
    11161 ccagcaccgc gcgggcaggc gcggtttgcg gatgtacctg accgatgacg tcctgggctt
    11221 cctgggataa aaagtgggta tagatcggat gtttcggcat cagttctgca ataaatgcct
    11281 tttgcccggt gccgcagaga aaatcggcgc ggctaaaatc catcgaaaag aagcgtttac
    11341 cgaggctttg ccagaacggt gaatagccgt gttcgtcaat caccccgcgc atttcagcaa
    11401 ccactttgtc attaaacttg tcgcgaaaag ccgccataaa cataaagcgc gatttcgaca
    11461 gcaaatagcc gttgccctct ttgcgccagt ccgggtcgag aaacagcgtg cacagctcgc
    11521 tgctgccggt gtgatcgtta ctaagaaaca gcgtcggcaa tgcgttatag acattcagct
    11581 cttttgaggc gtgaaccaac gtgccgacgc gatagttgta ccagggatcg ttcagcccaa
    11641 ccgccacctc aatagcgcaa atccccgcca cggtgcctgt ctcgctatct tccagcacga
    11701 acacatagcc ctgctcgctt ttgggcagtt caccttgcca ggttttgatt gccctttcga
    11761 tacgcgccga aagcgtggct tcattggcgg gaagcgacgt caggccgccg cccgttttgc
    11821 tggcaagctg catcagcgcc gagacatctg agcgctcaac gggacggatg accatcatga
    11881 tgaacctcgg ctaacaaagt gttcgcaagc agctgcaaag cgatccagtc cggtcgtcac
    11941 ctcttcttcg ctgacattga gcgcaggcgc aaaacgcacc acgttgccac ccgcaatcag
    12001 taccatcacg cctgctttcg ccgcttccag agagatctgt ttcgcttgcc cggcgtaatc
    12061 ggcattcagt acacagccaa tcaacaaacc taagccgcga acttcactga acaaaccata
    12121 gcggtgatta atagtattaa gacgctcaac aaaccagtcg tgacgctgtt taacgccatt
    12181 aagcatctct ggtgtgttga tgagctccag cactttgcct gctaccgccg aggccagcgg
    12241 attaccgcca taggtggtgc catgagtgcc aacggtcatc acgcgggcgc actcttcggt
    12301 tgccaacaac gcaccgaccg ggaaaccgcc gcccagcgct ttggcggtgg ttaacagatc
    12361 gggcgtcacg ccgtagtgca tataggcata cagctcacca gtgcgcccga cgccggtttg
    12421 tacctcatca aaaatcagca aggcattgtg gcggtcacac aattcacgca gaccttgtaa
    12481 aaacgcgttg ctggctggca ccacaccgcc ttcgccctag atgggctcga caatcaccgc
    12541 acaggtggcg tcgtcaatca gcgcgctggc agagttaata tcgttatatg cagcatgacg
    12601 aatatccgcc ggcagtggcg caaaatcctg tgaataggct ggctgcccac ccgcactgac
    12661 agtaaacagc gtgcgaccat gaaacgcatt tttgaacgcc acgatgccac tcttatggct
    12721 gccgtagcgg tcgtgagcga atttacgcgc cagttttagc gccgcttcgt tggcttccgc
    12781 accggagtta caaaagaaga cgcgatcggc aaacgtggcg tcgatcaatt ttttcgccag
    12841 tcgcagtacc ggctcgttgg tgtaaccgtt gccggtatgc cagaacttac tcgcctgttc
    12901 gttcagcgct tcacgcagtt ccggatgcgc atggcccagc gcgttcaccg caatgccacc
    12961 cgcgaagtcg atatactctt tcccctgctg atcccacaag cgcgaacctt cgccacgtac
    13021 cggtataaag ggtgccggag cgtaaacagg tatcatccat tcatcaaagt tttcacgcgt
    13081 aattggctga gacatagcga cctctacagt aaataattcg ttatttgtat gttaacaata
    13141 agtaatgttt gcgctgtaaa tgtagattgc agggttcgtg ccagccagtg ataaaagtgc
    13201 ataaacggcg gaggctaact ggaaatcaag gagttataac caaaccatat gcatttaaag
    13261 tgcatataaa gtgaatacgt ttgcgatgtg ggtgaataaa aagaataaaa aacgcaatgt
    13321 tatgcagaag taaaatataa ttctagaatt gtgatcattg acgaaattta ctggaaatta
    13381 ctgcgccatt ctgacgcagc gcgcaccaaa agcgggcatt ttttgcgcca tcgttgacat
    13441 cattaacaac catcgatcaa atcacttaac aacaggcggt aagcaacgcg aaattctgct
    13501 accatccacg cactctttat ctgaataaat ggcagcgact atgaaatttg tctcttttaa
    13561 tatcaacggc ctgcgcgcca gacctcacca gcttgaagcc atcgtcgaaa agcaccaacc
    13621 ggatgtgatt ggcctgcagg agacaaaagt tcatgacgat atgtttccgc tcgaagaggt
    13681 ggcgaagctc ggctacaacg tgttttatca cgggcagaaa ggccattatg gcgtggcgct
    13741 gctgaccaaa gagacgccga ttgccgtgcg tcgcggcttt cccggtgacg acgaagaggc
    13801 gcagcggcgg attattatgg cggaaatccc ctcactgctg ggtaatgtca ccgtgatcaa
    13861 cggttacttc ccgcagggtg aaagccgcga ccatccgata aaattcccgg caaaagcgca
    13921 gttttatcag aatctgcaaa actacctgga aaccgaactc aaacgtgata atccggtact
    13981 gattatgggc gatatgaata tcagccctac agatctggat atcggcattg gcgaagaaaa
    14041 ccgtaagcgc tggctgcgta ccggtaaatg ctctttcctg ccggaagagc gcgaatggat
    14101 ggacaggctg atgagctggg gggtggtcga taccttccgc catgctaatc cgcaaacagc
    14161 agatcgtttc tcatggtttg attaccgctc aaaaggtttt gacgataacc gtggtctgcg
    14221 catcgacctg ctgctcgcca gccaaccgct ggcagaatgt tgcgtagaaa ccggcatcga
    14281 ctatgaaatt cgcagcatgg aaaaaccgtc cgatcacgcc cccgtctggg cgaccttccg
    14341 ccgctaattt agcagctctc ctggctcaaa ctgggtcagg agaattaacc ttgagaaaaa
    14401 tcaacaaact gtcagtaatg atttgttgcc tgccgtcctt tgttataccg tcactgcgtt
    14461 tttagttgtc tgaccacttc tctattatca agtttgatat aggaaactcc acgatgaacg
    14521 ctgagcgtaa ctttctgttt gcctgtctta tttttgcgct ggtcatttac gctatccacg
    14581 ctttcggttt attcgatctg ctcaccgatt taccccactt acagacactc attcgccaga
    14641 gcggattgtt cggttatagc ctctatattc tgttattcat caatgccccc ctctttctgt
    14701 taccaggaag catactggtg atcgctggag gaatagtttt tggcccgctc ttagggacac
    14761 aactctcatt aattgccgca acgctggcct cctcgtgctc attcctgctg gcgcgctggc
    14821 tgggacgtga tttactgctg aaatacgtgg gccatagcca taccttccag gccattgaaa
    14881 aaggcattgc gcgtaacggt attgattttc ttattctgac ccgcttaatc ccgttgtttc
    14941 cttacaatat tcaaaattac gcttacggat tcaccaccat cgccttctgg ccttataccc
    15001 ttatttcggc actcacgacc ctgcccggta ttgttattta taccgtgatg gcaagcgatc
    15061 tcgccaatga aggcattacg ctgcgcttta ttttacaact ctgtctggcg ggcctggcgc
    15121 tgtttattct catccagctc gcaaaactct acgcccgaca caaacatgtg gatctgtctg
    15181 cttcgcgccg cagcccactt actcacccaa aaaatgaagg atagaacgat gtcgcaacat
    15241 tattcagtgt catggaaaaa aggactggct gcactctgct tactggctgt tgccgggctt
    15301 agcggctgcg atcaaaaaga gaatgcggcg gcaaaagtgg aatacgacgg actttcgaac
    15361 agccaaccgc tgcgcgtcga tgccaataac catacggtaa ccatgctggt gcaaattaat
    15421 ggtcgtttcc tcaccgacga cactcgtcac ggtattgtgt ttaaagatgg ctccaacgga
    15481 cataaatcgc tgtttatggc ttatgcgccc ccgaaagcat tttatgaagc cctgaaagag
    15541 gcaggtggta cgccgggcga aaacatgacg atggataata aagaaacgac tcatgtcaca
    15601 ggcagcaaac tggatatttc ggtcaactgg caaggggcgg caaaagcgta ttccttcgat
    15661 gaagtgattg ttgacagtaa tggcaagaaa ctggacatgc gctttggcgg taatttaacg
    15721 gcagcagaag agaagaaaac gggttgcctg gtgtgtctgg atagctgccc ggtcggcatc
    15781 gtcagcaatg caacatacac ttatggtgcg gtggaaaaac acggcgaagt gaaattcaaa
    15841 ggcaatgcct cagttctccc ggcggataac acgctggcaa cggttacctt taaaatcacc
    15901 gaataaagcc aagataaagg atgatgaaga tacaatcgcg taaaatctgg tactaccgta
    15961 taaccctcat catcctgttg ttcgctatgc tgctcgcatg ggcgctgctt cccggcgtcc
    16021 atgagtttat caatcgcagc gttgcggcgt ttgccgccgt ggaccaacag ggtatagaac
    16081 gctttattca gtcttacggc gcactggcgg cagttgtctc gttcttgttg atgattttac
    16141 aggccattgc tgcaccgcta cctgcgtttt tgatcacctt tgccaatgcg tcgctgtttg
    16201 gcgcgttctg gggcggcttg ctgtcgtgga ccagttcgat ggccggcgcg gcgctgtgct
    16261 tttttatcgc cagagtgatg ggccgcgaag tggtggaaaa attaaccggc aaaaccgtgc
    16321 ttgacagtat ggacggcttt ttcactcgct acggcaaaca caccatcctg gtctgtcggt
    16381 tattgccttt tgtccctttc gatccaatca gctatgctgc cggtctgact tcaatacgtt
    16441 ttcgctcgtt ttttatcgcc accgggcttg gtcaattacc ggcgactatt gtttattcct
    16501 gggcaggcag catgttaaca ggcggtactt tctggtttgt caccgaactg tttattctgt
    16561 ttgccctgac cgtggtgatt tttatggcga agaaaatatg gcttgagcgc cagaagagga
    16621 atgactgatg ggtttaccgc cgcttagcaa aattcctttc attttacgtc cgcaggcgtg
    16681 gctgcatcgt cgccattacg gcgaggtgct aagcccgatt cgctggtggg ggcggatccc
    16741 gtttatcttt tatttggtgt caatgtttgt tgggtggctg gagcgcaaac gctcaccgct
    16801 cgatccggta gtgcgatcgc ttgtcagcgc gcgcattgcg caaatgtgcc tgtgtgagtt
    16861 ttgcgtggat atcaccagta tgaaagtcgc cgagcgtacc ggcagcaccg ataaactgct
    16921 ggcagtggct gactggcggc aaagcccgct ctttagcgat gaagaacggc tggcgctgga
    16981 gtacgccgaa gccgcaagcg tcacaccgcc aacagtcgat gatgccctgc gtacccgact
    17041 ggctgcgcat tttgacgctc aggcgctcac cgaactgacg gcattgatcg gcctgcaaaa
    17101 cctgtcggct cgttttaatt ctgccatgga cattcccgct caggggctgt gccgtattcc
    17161 ggaaaaacgt tcttaaggag ggaagatgcg ccattgtggg tggttgctgg gattgttatc
    17221 gctgttttct ttggcaacac atgccagtga ctggcaggaa attaaaaatg aggccaaagg
    17281 gcaaaccgtc tggtttaacg cctggggcgg cgataccgca attaaccgct atctcgactg
    17341 ggttagcggc gagatgaaaa cccattacgc tataaacctg aagattgttc gtctcgcgga
    17401 tgccgcagac gcggtgaagc gcattcagac cgaagctgct gccggtcgta aaacgggcgg
    17461 ctcggtggat ctgctctggg tgaacggcga aaacttccgc accttaaaag agtaatgcca
    17521 gtcagttaag caact
//
LOCUS       DAJTQQ010000080        17456 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000080 DAJTQQ010000000
VERSION     DAJTQQ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.080"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(33..764)
                     /gene="rlmB"
                     /locus_tag="OO309_002934"
     CDS             complement(33..764)
                     /gene="rlmB"
                     /locus_tag="OO309_002934"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR5848003.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(944..3385)
                     /gene="rnr"
                     /locus_tag="OO309_002935"
     CDS             complement(944..3385)
                     /gene="rnr"
                     /locus_tag="OO309_002935"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR5848004.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3424..3849)
                     /gene="nsrR"
                     /locus_tag="OO309_002936"
     CDS             complement(3424..3849)
                     /gene="nsrR"
                     /locus_tag="OO309_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR5848005.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4054..5352)
                     /gene="purA"
                     /locus_tag="OO309_002937"
     CDS             complement(4054..5352)
                     /gene="purA"
                     /locus_tag="OO309_002937"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR5848006.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5456..5653)
                     /locus_tag="OO309_002938"
     CDS             complement(5456..5653)
                     /locus_tag="OO309_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR5848007.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5735..6739)
                     /gene="hflC"
                     /locus_tag="OO309_002939"
     CDS             complement(5735..6739)
                     /gene="hflC"
                     /locus_tag="OO309_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR5848008.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6742..8001)
                     /gene="hflK"
                     /locus_tag="OO309_002940"
     CDS             complement(6742..8001)
                     /gene="hflK"
                     /locus_tag="OO309_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR5848009.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8087..9367)
                     /gene="hflX"
                     /locus_tag="OO309_002941"
     CDS             complement(8087..9367)
                     /gene="hflX"
                     /locus_tag="OO309_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR5848010.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9443..9751)
                     /gene="hfq"
                     /locus_tag="OO309_002942"
     CDS             complement(9443..9751)
                     /gene="hfq"
                     /locus_tag="OO309_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR5848011.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9837..10787)
                     /gene="miaA"
                     /locus_tag="OO309_002943"
     CDS             complement(9837..10787)
                     /gene="miaA"
                     /locus_tag="OO309_002943"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR5848012.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10780..12627)
                     /gene="mutL"
                     /locus_tag="OO309_002944"
     CDS             complement(10780..12627)
                     /gene="mutL"
                     /locus_tag="OO309_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR5848013.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12637..13974)
                     /gene="amiB"
                     /locus_tag="OO309_002945"
     CDS             complement(12637..13974)
                     /gene="amiB"
                     /locus_tag="OO309_002945"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR5848014.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13993..14454)
                     /gene="tsaE"
                     /locus_tag="OO309_002946"
     CDS             complement(13993..14454)
                     /gene="tsaE"
                     /locus_tag="OO309_002946"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR5848015.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14426..15973)
                     /gene="nnr"
                     /locus_tag="OO309_002947"
     CDS             complement(14426..15973)
                     /gene="nnr"
                     /locus_tag="OO309_002947"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR5848016.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15972..17111
                     /gene="queG"
                     /locus_tag="OO309_002948"
     CDS             15972..17111
                     /gene="queG"
                     /locus_tag="OO309_002948"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR5848017.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17094..17147)
                     /locus_tag="OO309_002949"
     CDS             complement(17094..17147)
                     /locus_tag="OO309_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848018.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17381..17456)
                     /locus_tag="OO309_002950"
     tRNA            complement(17381..17456)
                     /locus_tag="OO309_002950"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17421..17423),aa:Gly,seq:gcc)
BASE COUNT         3957 a         4980 c         4388 g         4131 t
ORIGIN      
        1 aaatctggat ggcttttcat aattcagagg aactagctgc gctgacgaac cgcttcaaat
       61 aagcaaattc cggttgcaac cgaaacgttc agggaagaaa cacttcctgc cattgggatg
      121 ctgatcaact catcgcaatg ttcacgggtc aggcgacgca taccttcacc ttccgcgccc
      181 atcaccagcg ccaggcgtcc ggtcattttg ctctgataga gtgtatgatc cgcctcgcct
      241 gccgtaccga cgatccagat attctcttcc tgcaacatac gcatggtgcg cgcaaggtta
      301 gtcacccgaa tcagcggaac gctttctgcc gcgccgcagg ctactttttt cgccgtagcg
      361 ttgagctgtg cggagcgatc tttcggcaca atcaccgcat gaacgcctgc ggcgtccgca
      421 ctacgcaggc acgcgccgag gttgtgcgga tcggttacgc cgtcgaggat cagcaggaac
      481 ggttgatcga gcgaagcgat cagatccggc agatcgtttt cctgatactg acgtcctggc
      541 ttcacgcggg cgataatgcc ctgatgcacg gcaccgtcgc ttttctcgtc gagatattgg
      601 cggtttgcca actggataac cacgccctga gactcaaggg cgtgaatcag cggtaacaga
      661 cgtttatctt cacggccttt taaaataaag acttcctgaa aacgttcagg ggcgcgctcc
      721 agcagggcct gcactgcgtg gatgccgtaa atcatttcgc tcattaatgt actcgttgtt
      781 tacgttttgc ctgatgcact acgtttatcc ggcctacatg atctctgcaa tatattgaat
      841 ttgcgtgatt atgtaggccg gataaggcgt tcacgccgca tccggcatga acaaagcgca
      901 ttttgtcagc aatctaaccc tcttctttta aagagggtat tgatcactct gccacttttt
      961 tcttcgccgc acgcttcgct ttggtcgctg cggctatttt ctgcgttttc gccgatggct
     1021 ttttcgcttt tctcgcgtct ttcttcgccg ctttcggctt cgtttttttc tcaccgcgga
     1081 aggcgctgtc tggctcaaag tttacctttt taccgacctg acaacgcttg ccgccttttt
     1141 tgcctgcatc gccttttttc gctttctcgc gcgccgtttt accgacgttg cgcggtgcgc
     1201 gttcactgga gatcagacta aagtcgattt tgcgctcgtc catattaacc gcttcgacgc
     1261 gaacttccac gcgatcgccc aggcgataag tctggccgct ggattccccc atcaggcgtt
     1321 gccctacctg gtcaaagcga tagtagtcat tgtccagcga agagacatgg accagaccat
     1381 caatgaacaa gtcgtccaga cggacgaaga agccaaagcc agtgacgctg gaaattacgc
     1441 ctttaaagac gttacctacc tggtcgagca tgaagtcaca cttcagccag tcagcgacat
     1501 cgcgcgttgc ttcgtcggca cgacgttccg ccatcgaaca gtgctgaccc agttgcaaca
     1561 tctcttccat cgaataatgg tagccgccgg tttcagtggt gttgccctga tgcccctgct
     1621 ctttcgccag cagatattta atggcgcggt gcagcgtcag gtcaggataa cgacgaatcg
     1681 gcgaagtaaa gtgcgcatag gactgcaatg ccagaccgaa gtgaccacgg ttttctggat
     1741 cgtaaatcgc ctgtttcatc gagcgtagca gcatggtttg cagcatttct gcatcaggac
     1801 ggtcggcaac cgactccagc agttccgcgt agtcacgcgg ttccggcttg ttaccacccg
     1861 gcagctccag ccccagctcc gccagcactg aacggaaaga ggtaatcgct tcggtgctcg
     1921 gcttgtcgtg aatacggaac agtgccggtt ctttcgcttt ctcaacgaaa cgcgccgccg
     1981 agatattcgc cagaatcatg cactcttcaa ttaatttgtg cgcgtcgtta cgctgggtct
     2041 gttcgatacg ttcaatacgg cgttcagcgt tgaaaatgaa cttcgcttct tcgctctcaa
     2101 atgagatccc accgcgttct tcacgggctt tatccagcac tttatagagg ttatgcaact
     2161 cttcgagatg cttaaccagc ggggcgtact gctcgcgcag atcctgatcg ccctgcagaa
     2221 tatgccagac tttggtgtag gtcagacgcg cgtgagagct catcaccgct tcgtagaatt
     2281 tgtagcccgt caggcggcct ttcgacgaaa ccgtcatctc gcacaccata cacaggcggt
     2341 ctacctgcgg gttgagcgaa cacaggccgt tagagagcac ttccggcagc atcgggataa
     2401 cctgcgaagg gaagtacacc gacgtgccac ggttacgcgc ttctctgtcc agcggcgttg
     2461 gcggacgcac atagtagctg acgtcggcaa tcgcgaccca taaacgccag ccgccgccgc
     2521 gttttttctc gcagtaaact gcatcgtcaa agtcacgggc gtcttcgcca tcaatggtga
     2581 ccagcggtaa atcgcgcaaa tcgacacggc ccgcttttgc ttcttccggc acttcttctt
     2641 tcagccccgc aacctgttgc tcaacagcct gcggccagat atacggaatt tcatgggtac
     2701 gcagagcgat atcaaccgcc atgccggtgc ccatattgtc gcccagcact tcgacgattt
     2761 tacccaccgc tttggtgcgg cgagtcggac gctgagtcag ttcgactacg accacaaagc
     2821 ccatccgcgc gcccatgatc tgatcgggcg ggattaagat atcgaagctc agacggctat
     2881 cgtcaggaac cacaaagccg acgcccgctt cggtaaagta gcgaccaaca atctggctgg
     2941 tttttggcac cagtacgcgg acaatacgcg cttcacgacg acctttacgg tcagcaccca
     3001 gcggctgcgc cagcacctga tcgccatgaa tgcaggtttt catctgctcg ctggagagat
     3061 acaaatcatc tttacgccct tcaacccgca gaaagccgta gccatcacgg tgaccaataa
     3121 cggtaccttt caccaggtcg aggcgttccg gcagcgcata gcactgacga cgagtgaaga
     3181 ccagttgacc atcgcgctcc atcgcgcgca ggcggcgacg caggccttca agctgctctt
     3241 cgccttcaat gtgcagttct accgccagct catcacggct ggccggtttt tcacgtttgg
     3301 ttaaatgttc gaggataaat tcccgactag ggatgggatt cgcgtatttt tcagcttcgc
     3361 gttcctggaa aggatcttgt gacatctcgg ttcctccgtt gtcatctctg atgaagattt
     3421 tcgtcactcc accagcaata atttataaag cggttgattc tcttcaacca aatcggcaag
     3481 cgtgtagtta tccagttccg taagaaaact ttgcacggcc ttagaaagcg cctgtttcaa
     3541 ccgacaggca ggtgtaatgt ggcaaaactc actgctgcaa ttcaccagcg ataagggctc
     3601 cagctcgcgc accacatcac caatacgtat cgcactcgcc ggtttaccca ggcgaatgcc
     3661 gccatttttt ccacgaacag cagtcacgta gccggcacga ctaagttgat tgattatttt
     3721 gaccatatga ttacgggaga cgccgtagac gtcagtcact tcagaaatac tggtcatccg
     3781 cccttctggc aatgacgcca tgtagatcag cgcacgtaat ccgtaatcag tgaaactcgt
     3841 taactgcaca tcaacctcaa aagggaaatc gggaaaaaat acatttatat tgatgataaa
     3901 ccagccacaa gctgtgtcgc taatttattt cagttcgggg aaggaaaaaa gcgaggattt
     3961 aacactgtgt cggatagcgt aatggcttaa ccgacacagc aatgtatcac actgttagcc
     4021 cggcaggcaa aatatctgcc aggcgtacca gaattacgcg tcgaacgggt cgcgcagaat
     4081 catggtttca gtacgatccg gaccggtaga gatgatatcg atcggcacac cggtcagctc
     4141 ttcaatacgc ttgatgtagt tcagcgccgc ctgcggcagg ccgctacgat ctttcacgcc
     4201 gaaagtggat tcagaccagc ccggcatggt ttcgtaaatc ggctctacac ctttccagtc
     4261 gtcagctgcc agcggagtgg tagtcacttc gcgaccatcc ggcatacggt aagccacgca
     4321 gagtttaacc tctttcaggc catccagaac gtccagttta gtcaggcaga agccagacag
     4381 ggagttcagc tgtaccgcac gacgaacggc aacggtgtcc agccagccgg tacgacgacg
     4441 acgacccgta gttgcaccga attcgttacc ctgcttgcag aggaactcgc cagtttcatc
     4501 aaacagttcg gccgggaaag gacctgcacc tacacgagtt gagtaagctt tgaggatacc
     4561 cagaacgtaa tcaacataac gcgggcccag gccggaaccg gtcgccacgc caccagcagt
     4621 ggtgttggaa gaagttacgt acggataagt accgtggtcg atatccagca gcgtaccctg
     4681 cgcaccttcg aacatgacga aatcgccacg ctgacgcgcc tggtcgagca ggtcagaaac
     4741 gtcaaccacc atagaagtca ggatgtcggc aacagccatc gtatcatcca gaactttctg
     4801 gtaatcaacc gcttcagctt tgtagtagtt aaccaactgg aagttgtgat attccatcac
     4861 ttctttcagt ttttcagcga aggtttcttt gtcgaaaagg tcgccaacac gcagaccgcg
     4921 acgtgccact ttatcttcat aagcaggccc gataccacga ccggtggtgc cgatcgcttt
     4981 cgcgccacgc gctttctcac gcgcgttatc cagagcaacg tgataatcaa ggatcagcgg
     5041 acatgcttca gacagcagca gacgctcacg aacggggatg ccacggtctt ccagttcttt
     5101 catctctttc atcagcgcgg ccggagacag cacaacaccg ttaccgatga tgctggttac
     5161 attctcgcgg agaatacctg atggaataag atggagaacg gttttttcac cgttgattac
     5221 gagagtatgg cctgcgttgt gaccgccctg gtagcgtaca acatatttag cccgttcagt
     5281 cagaagatcg acgatcttac ctttaccttc gtcaccccat tgggtgccca gtacgacgac
     5341 gttgttaccc atttttcaaa atcaccgttt gcttaaaaat ggattctacc atcgcttttt
     5401 cagagttaca gcactttttg cactcaaaaa tgacctcgat cagtctgttt tttgttcagc
     5461 caatcgtttt cctcaacatg tagtagacca caacgcccgc aaccacaagt ccaccgccaa
     5521 aacgacgtaa aatattatcg ggcaaattgg tcatcgcaga gatcatcttc ttccatgcct
     5581 tcgggtaaag catcggccct aaaccttcca gtaccaaaac cagggcaagc gccagccaga
     5641 ttgtcgaatt cattcattat ccttatagaa aaagaaaacc accgacatcc ctgaggatgc
     5701 ggtggcttta ttgacctgta ccgcagtcgt tatattaacg cgttgcggaa gtcggcgtct
     5761 tcatgtagcg gaagaaatcg ctatccgggc tcatgaccat cacgtcctga ttgccagaga
     5821 agctgttctc ataagcacgc aggctacgga tgaatgcgta gaagtccgga tctttactga
     5881 atgcatcagc aaacagtttg gctgcttcgg catcgccttc accacgcata atgcggccct
     5941 gacgctcagc ttctgccagc gttctggtca cttcatagtc ggcagtcgcg cgcagttttt
     6001 ccgcttcttc ctgaccttgt gaacggtgac gacgcgctac cgcttcacgc tcggcgcgca
     6061 tacggttgta gatcgcttca gacacttcgg tcggcaggtt gatctgcttg atacgcacat
     6121 cgacaacttc aatacccagc gccgccatac tgttcgggtt gatgaccgga actttgccct
     6181 tcgtctcagc cgttacgcgc tctgccgctt cggcaatggc gttatctgct gccggggtag
     6241 taacttcatc ttctgtaccc gcagaaccgg agttcagcgc gtcacgtact tcgagggtca
     6301 gacgaccacg ggaatcggtg acgatatctt tcacgtccag gcgaccaatt tcagaacgca
     6361 gacggtcaga gaacttacgt ttcaacagca cttccgcttg cgaaatgtcg ccaccacccg
     6421 ttgccaggta gtaacggctg aaatcgctga tgcgccattt gatgtaagag tcgacgatca
     6481 ggtctttctt ctctttggtc acaaagcggt cggcctggtt gtccatggtc tgaatacgtg
     6541 cgtcgagcat tttcaccgtt tcaatgaacg gtatcttgaa atgcagacca ggctcataaa
     6601 ccaaaggttt gttgtcatcg tcacgcagta ccttaccaaa acgcagcgta ataccgcgct
     6661 caccttcttt gacgacaaag acagacatgt aaagcactac cagcacgatg atgataatcg
     6721 cgataactga cttacgcatc gttattcccc ctgacgctgg tagtcgttac gctgcgcgtt
     6781 ggcgcggcgt tggtccataa tatcgccctg actggtggac gacgtgttgc ttgctccact
     6841 ggttgtggag gaagaggctg gcggcagacg cagcagattg ctggcaccgt tatcgctctt
     6901 cgccgcaggg gcgttaccac ctttcagcat ctggtctaac ggcagaacca tcaggttgcc
     6961 acctttatcg ttaaccagca ctttgcgggt gttacccaac actttttcca tcgtctcgat
     7021 atacagacgc tcgcgagtaa tttccggcgc ggctttatat tccggcagaa gtttagcaaa
     7081 gcgcgccact tcaccctgag cttccaggat ggtctgggcc ttgtacgcac gcgcctcttc
     7141 gaggatacgt tgcgcctgac cgttcgcacg cggctgaact tcgttggaat acgcttctgc
     7201 ttcacgaatg tattgctgtt cgttttcacg cgcggcaatc gcatcgtcaa acgccgcttt
     7261 tacttcttcc ggcggacgag cagcctggaa gttgacgtcc agcagcgtga tacccatgtc
     7321 atacggacga atcgtctctt ccagttcgcg ctgagtatcg ctacgaatca cggtacgacc
     7381 ttccgtcaga atgcggtcca tggtgtattt accgataact ccacgcaggg cgctgtcggt
     7441 agcctgacgc aggctgtcat ccgggctggt cacgctatac agatattttt ccggattggt
     7501 gacgcggtac tgcacgttca tctcaacgcg cactacgttc tcgtccgacg tcagcatcac
     7561 accagaagcg gccagttcac gcacggcttc cacgttcacc ggtttgactt cgtcgataaa
     7621 cgtcggtttc cagttcagac ccggctcaac cagatggctg aatttaccaa agcgtgttac
     7681 cacgccgcgt tcggcttctt taatggtata gaaaccactg gccgcccaga taatgacaat
     7741 cgctgccgct gcgatggtaa cgacacgacc gccaagctgc gggcgcgggc cttgcgatga
     7801 actgccaccg ccagatccgg tgcctttacc gccgcccaga ccaccgagct ttttgctcag
     7861 tttgcggaag atatcatcta aatcaggtgg cccttgatcg cgaccgcctt tgtttccatt
     7921 tccctcagag ttgccgccag gtttgctgct tccccacggg tcgcggtctt gtccgttatt
     7981 accgggctga ttccacgcca tgtttgtgct ccatatttgt tatgcggtga tccctgttgt
     8041 ctttcgacgc cagaggatat gactccacgc ttcagacgct acgccgttag atcaggtaat
     8101 cgatcaacgc cggttcttgt ttacagaggc gacgccagtc aacgatcggc atacgaactt
     8161 gcagacttac gctgccgtcc tcctccatcc actctttttc tattgcctga agctgataaa
     8221 aacgactcct cagacgccct tcctgcggtg gcagacgcaa tgtatgctgc gccacctcgc
     8281 cggaaagccg ctccgtcaaa gcctgaaaaa gctgtggtat ccccgctccg gtctgtgcgg
     8341 aaagccagac acggatcggt ttgttctctt cgtcccgatc aatacgcggt tcgaaatctt
     8401 ccagcatatc gatcttgttc atcaccagca gggttgggat ctcgtgagcg tcgatctctt
     8461 caagaaccgt attcaccgct tcgatgtttt cttgtacacg cacatccgcc gcatcaatga
     8521 cgtgcagcag taatgtggct tgccgcgtct cttgtaacgt ggctttaaat gccgccacca
     8581 gatcgtgcgg caggtggcga ataaacccta cggtatctgc aagtacggtt tcaccgacat
     8641 ctgcaacgtc aatacgccgc aacgtcgggt cgagggtggc aaacaactgg tctgccgcgt
     8701 agacccgcgc ttcggtgatg cgattgaaaa gggtagattt accggcgttg gtatatccca
     8761 ccagcgaaac agtaggaacg tcggctttga tacgcgattg ccgcccctgc tcacgctgct
     8821 tttcaactct ttccaggcgc gactgtatct gcacgatgcg attacgcaac aaacgacggt
     8881 cggtttcgag ctgggtttca cccggaccac gcaaacctat cccgcctttc tgtctttcaa
     8941 ggtgggtcca gccacgcacc aggcgcgtag ccagatggcg cagctgcgcc agctcaacct
     9001 gcaacttacc ctcatgggta cgcgcacgtt gggcgaaaat atctaaaata aggccggtgc
     9061 ggtcgataac acgacactcg cacaaacgct ccaggttacg ctcttgcgcc gggctcaggg
     9121 catggtcaaa aagaacgacc gaagcaccca tcgctttgac agcttccgca atttcaactg
     9181 ctttaccttc acctacaaaa tactttgggt gcggcgcttt acggctaccg gtaatcacct
     9241 gcaatgcttc gacaccggcg gaagagacca gagattcaaa ctcctggagg tcttccatat
     9301 ctttgtcttg cgtaaaatag atgtgtacca gtaccgcctg ctcaccagca tcataacggt
     9361 caaacaagcg tataaccctc taaatagatc agcggggaac gcaggatcgc tggctccccg
     9421 tgtaaaaaaa cagcccgaaa ccttattcgg tttcttcgct gtcctgttgc gcggaagtat
     9481 tctgcgcgct gctaccatga tggtagttac tgctggtacc gccaccggcg ttgttactgt
     9541 gatgagaaac cgggcgagat gggacaacag tagaaatcgc gtgcttgtaa accatctggc
     9601 tgaccgtgtt tttcaacagg atcacgaact gatcaaaaga ctcgatttgc ccttgcagct
     9661 taataccatt caccaaataa atagaaactg gaacacgttc ccgacgcagt gcgttcagga
     9721 acggatcttg taaagattgc cccttagcca ttctctcttt tccttatatg cttatttgta
     9781 ctttgaacct ttcgattctg aaaaaattgc gcacgatacg tctcaattgt acacattcag
     9841 cctgcgatag caccaacaac ctgtaatact tcgtcacgcg cctgttctgg tttttcactg
     9901 tcaagccagt gaaccccttc ccaaccacgc agccaggtta tctgccgctt cgccaactgt
     9961 ctcgtggcgc aaacacctcg ataaaccatt tcatcgtatg agatttcgcc ttcaaggtaa
    10021 gaccacatct ggcgataacc cacgcaacga atggaaggca agtccgtatg caaatctcct
    10081 cgggcaaaaa gcgcccggac ttctgcttca aaacctgaag ccaacatctg atgaaaacgc
    10141 tgctcaatgc gttgatggag cagttcacgg ctcgccgggg cgatggcgaa ctgatgcacc
    10201 tgatacggta gagcgtctcc tgacgtttgc gtcagttccg ttaaagtttt acccgaaatg
    10261 aaaaaaactt ccagtgcccg ggaaagcctt tgtggatcat ttggatgaat ccttgctgcc
    10321 gcaaccggat ctacctcctg aagttgacga tgcaatgact cccaaccttg ctctgccgcc
    10381 tgttgctcaa ttctggcccg cacttccggg tctgccgacg gtagcggcga caacccttcc
    10441 agcaatgcct tgaaatacaa catcgtaccg cccactaaca gtgggatccg ccccgccgcg
    10501 gtgatatcgg ccatttccgc cagcgcatcg cggcgaaaat cagcagccga gtaagcctgc
    10561 gacggatcgc gaatatccag caatcggtgc ggcgcggcga gtaactcttc agcgttcggc
    10621 ttcgccgtcc cgatatccat ccctttgtaa ataaggacag aatcaacgct tatcaactct
    10681 actggtaaaa ttttacgcag ctcaatggct aacgccgttt taccggaggc cgtcggcccc
    10741 atcaaaaaaa tcgccttagg caggctcgcc ttactgatat cactcatctt tcagggcttt
    10801 tatcgccgga tgtaaatcaa cagattgtaa cagaccaccc ggcggcgttt tcacaagttg
    10861 cggacataac cgttccacgt ccgccagcag ggttatggcc tgtgccattg accactgcgc
    10921 atgttcgctc atcagatttc gtgcaatcca ctgcgcaata ttgccaggtt cgaatacgga
    10981 ctgcttcgcc aggtagccta tcagttcagg aatcaagatt tgtaaatttt gttggcgtaa
    11041 gggtaaaggc acggccctga tggtcacatg ctgtgcatct gactggaaat caatacccaa
    11101 ttccgctagg gcagactgcg ctttttctaa tgccgatttt tcttcgccag aaacttttag
    11161 ccgcaacgga atcagcagcg gctgggcgca aacgggcgct tcacccggcg tcaattgtgc
    11221 ctgacgcagc caacgttctg ccactggcaa ggataaaagt gaaatgttgc catcgcgctc
    11281 cagcaacgca cagtcggaat ggacgatagt cagtacccga ccaaaactct gactgttcgc
    11341 cgcaagtgca ggttcctgcg gttccggcgc ttttggtttt tgcatcggcg cgggcgtttg
    11401 caaaagctgg cgatacactt caccttgctg tttctggtag cctggctgcg cattcggcca
    11461 gggggcagcc ggacgactgc ctgaggcggg cgcaggagag tagcgcggag ctaccggctc
    11521 acgagctgcc ggttctgcaa agtgattgcg ccccgccgcc acgcggtttt ccgggatggc
    11581 acgcggtgcg ggttgtggtt catcgtccag cggtagcggc gtttccagtt gctgttgtag
    11641 cacgctcagc acgccctgat agataaaatc atgcaccaga cgcgactgat ggaaacgcac
    11701 ttcgtgtttg gcggggtgta cgttgacgtc cacctgatgc gggtcgatct ccagatacaa
    11761 cacaaatgcc ggttgctgat cggcccccag tttgtcttcg caggcctggc ggatcgcatg
    11821 attgatcagg cgatcgcgca tcatgcgacc attcacgtag caatactgaa tttccgccag
    11881 tgcgggcgta gtgtgatttg gatcggccac ccagccgcgc agggtgagat cgccgtgttg
    11941 ccattcaatc gccagcgctt gttcgagaaa agcggtgccg caaatcgcgc ctaagcgccg
    12001 ttctttttgc ccgccttccg gcactgcgcg gtactgacgc acaattttac cgttatgcga
    12061 caggttgatc gtgacgtcga aacgcgccag cgcaatgcgg cggatgattt catcaatatg
    12121 gctaaattcg gttttctcgg tgcgcaggaa tttgcgccgc gccggggtgt tgtagaacag
    12181 atccagcacc tccagcgtcg ttcccaccgg atgcgctgcc ggtttaaccg tcacgtccat
    12241 gtcgcgtcct tcggcatagg cctgccaggc ttcctgctgt tctgcggtgc gtgaagtgag
    12301 cgtcaggcgg gaaaccgaac tgatactcgc cagcgcctca ccgcgaaagc ccaggctgat
    12361 aatggcttcg agatcgtcca gagaggcgat tttactggtg gcatgacgag ccagcgccag
    12421 cgccagctca tcttttttga taccgcagcc gttatcacga atgcggataa gtttcgcccc
    12481 accgcgttcg atatcaatat cgatacgcgt cgcacctgca tcgaggctgt tttccactag
    12541 ttctttgact accgacgcag gtcgctcgac cacctcacct gcggcaatct ggttcgccag
    12601 ttgtggcggt aagacctgaa ttggcatcaa tcgtccttag tttggcagcg tgcgatctgg
    12661 cgtcgtcacc gtactggcag tttgtgccgt tgcaccctgc ggcgcagatt gcatcggatg
    12721 cgcaaggaag taattgcgca ggcctttgta aatggcttct gccagctgtt gttggtaatc
    12781 gtcgctcgcc agcaaacgtt cttcgctgtt gttgctgata aaaccggttt cgaccagtac
    12841 tgatgggata tccggcgagc gcagaacgcc aaggctggcg tgttctggtc gacgtttatg
    12901 tatttcgcca atgcgttgca actgactgat catactggtc gctacatcat accctacccg
    12961 ctgggaatga ccgaactgta aatccagcac cgcctggctt aaataggggt cagactgact
    13021 gttcgccagc acatcacccg ccccacccag cagctccgac agtttctcgt gctgctccag
    13081 ccagctggcc atttcactgt tggcgcgacg gttagagagc acccatacgg aagcgccagt
    13141 cgcactacgg ttcggtgcgg catcagcgtg aatcgacacg aggaaattgg cgttttgctt
    13201 atgtgccaca tcactgcgcc ccatcaccga gataaagtaa tccccgtcac gggttaaaac
    13261 gcctttaaac atcggatcgt cattgagcaa agtacgcaat ttacgcgcga tggcgatggt
    13321 gacatttttc tcccgcgtac caccggggcc gatagcgcca gggtcctgac cgccgtgtcc
    13381 ggcatcaata gcgatgataa ttttatcgcc agtgttagcc gtcgcgcgcg ctgccggacg
    13441 cgttaccgta ttactgctga taacacccgt agtgcggtta ctttccgttt taaacggatt
    13501 gcgcgccggt tcgctgacgc gcggtgcgac aaccgcaggc gtttcaacgc gtttcgcaac
    13561 cacgggcggc ggaggaggcg gtggcggcac atcggcgtta atcgtaaaga cgacagtgta
    13621 attgctgcca ttctgccgct tcaccgcttc ggttttaccg ttttcggtaa gatcgaccac
    13681 cagccgtagc gtttgtgcat ctttaggcgt tccagagcga atcgccttca ccagattatt
    13741 gccgctgaac aacaacggca gtccctgaat catgcccgtt tgtttgatat cgagcgccac
    13801 ggtgcgtttg ctttgatggc taaacgcata atcaggatcg ccaataaaac tcaacgttat
    13861 ccgcgcctgt tgattaccgt tagaaacctg aatatcagag agcgtcgcgg cacccaccgg
    13921 cgtgcacagc agcagcagcg tcgctaccaa ccaatttctg atgcgataca tcatcccgcc
    13981 acctttcaaa ggttaaccgg ctaaacgcgc cagcaacaat tcacccgcag aggaaaccgc
    14041 actcacgcgc gcctcacggc cttgtgcctg ataatcaatg tgtatttcga catccgggtc
    14101 aggaagaaca cctgtacctt gttgtggcca ctccaccagg cagatggcat cgttggcaaa
    14161 ataatcgcgg atccccataa actccagctc ctcgggatcg gcaaggcggt acaaatcaaa
    14221 gtgatagacc attaagttgt cgagcgtata gggttcgacc agcgtataag tggggctttt
    14281 gacattaccc tgatgaccca gagcctgtaa aaagccccgg ctaaaggtgg ttttacctgc
    14341 gcctaaatcg ccatacagat agattacggt tgcgccatcg caggctttcg ctacccgctc
    14401 gcccaggtct aatgttgcct gctcatcagg gagcggaatt actcgattca tcatggtttt
    14461 tatcagtcac ttccgggtta acaatacgct gtagcgtgga aaagagatcg gttgccagca
    14521 tcccgcgcgt tccaaaacgc gccgccagta cgtcagctgc cgcaccgtgc gcgacacagc
    14581 ctgcacaggc tgcatcatac ggcgacagtt tttgcccaag caatgcgcca ataataccag
    14641 agagcacatc gcccatgccg ccgctcgcca tgcctgcatt tccggcatca ataatgccta
    14701 aagcgtcagg atgggcggcg accacggttc cggcaccttt cagcaccgct acgccgccat
    14761 aacgttgtac cagacgtttg gcgcaatgta agcggtcact ttcaatttca gcgacggaac
    14821 agcctaacaa ccgtgcggcc tcgctaggat gcggcgtgat cacgcgattg tgacgcttat
    14881 cgggattgat tgccagcagg ttcaatgcat cggcatccca caacatcggt ttgcgaaaat
    14941 tctcaacttt ttgcagggct tttttccccc actcttgctg gcccagaccg ggaccaatca
    15001 ccaccacatc ggcccattcc aggctttcgg taagagagtc catagtcagt tcatgcacca
    15061 tcaattctgg tcgtgcagtc agcagcggcg caatgttctc actgcgggtc agtactcgga
    15121 ctaaaccagc accagcacgt agcgccgctt cccccgtcat acgaatagcc cccgccgtgc
    15181 cgtgatcgcc accgataatc accagccgcc cgtgatcgcc tttatgcgaa gtcgggcgac
    15241 gcggtttcag ccagtgagaa agttgttctg ccgaaaaccg ctgaattttc gtctcctgac
    15301 ctgccagcca gctatccagc cccagtgagt caaaatgcag ttgtcctgta acatcccgcg
    15361 cttttccagt gagcaagcct agtttcagcg caataaaagt gatggtgtga tcggcgttga
    15421 tcactgcgcc tggcgtagcg ccggtttcag ccagcaggcc ggaagggata tcaaccgcca
    15481 caatcggcgc aggatgggaa ttagcgtggt cgattaactg gctaatggat tcgcggggcg
    15541 cttgctgcaa gccggtaccg agcagcgcat caacaatcag atctaccgat tcgggccaga
    15601 caatattcga agcatggatc tcgccacccg cgtttaacca tgcttcgcgt gccagcgcgg
    15661 cctcttccgg caacggtttg tcgctctcct gggccaacaa cgtgacctca atgccgaccg
    15721 ctttggccag tcgcgcgacc acgtagccat cgccgccgtt attaccatga ccgcacagca
    15781 ccagccagtg gcgggcgtca ggatacgccg aacgacacac ctggaatgcg gcctcgccag
    15841 cgcgaagcat cagctcatag agtgtgagcc ccagcacatc tgccgcctcg cgttctccgc
    15901 ggcggatatc gtcggcgtac cagacggtgt gtggtatact tacggggttt ttcttcattg
    15961 tatggtccgt catgtcagag cccctcgatc tcaatcagtt agcgcaaaaa attaaacagt
    16021 gggggctgga actgggcttt cagcaggtag gtattaccga taccgatctc agcgagtccg
    16081 agcccaaact gcaagcatgg ctggacaaac aataccacgg cgaaatggac tggatggcac
    16141 gtcacggtat gctgcgcgct cgcccccatg agttattacc cggtacgctg cgtgtaatca
    16201 gcgtgcggat gaattacctt cctgctaacg ccgcatttgc cagcacgctg aaaaacccca
    16261 aactcggcta tgttagccgt tatgcgctgg gccgtgacta tcacaaactt ctgcgcaacc
    16321 gactcaaaaa gctgggcgag atgattcagc aacattgtgt ttcgctgaat tttagaccgt
    16381 ttgtcgattc tgcgcctatt ctcgagcgcc cgttagctga aaaagctggg ctcggctgga
    16441 caggtaagca ctcacttatc ctcaatcgcg aggccggttc gttcttcttt ttaggcgaat
    16501 tgctggtcga tattccgctg cccgtggatc aaccagtcga ggaaggatgc ggcaaatgcg
    16561 tggcctgtat gacgatttgc ccgaccggtg ccatcgtcga gccatatacc gtcgatgctc
    16621 gccgctgtat ctcttatctc accatcgaac ttgaaggggc gctcccggaa gagttgcgac
    16681 cgttaatggg aaaccgtatt tacggttgcg atgactgcca gcttatctgc ccgtggaatc
    16741 gctattcgca actcactaca gaagacgatt tcagcccgcg taagccgcta cacgcaccgg
    16801 aactcattga gttatttgcc tggagcgaag agaagttttt aaaagtcacg gaaggatcgg
    16861 cgattcgccg tataggtcac ctgcgctggc tgcgtaatat cgccgtggca ttaggcaatg
    16921 ccccttggga tgaaacgatt ttgacagcgc tggaaagtcg taaaggtgag cacccacttc
    16981 tcgatgagca catagcgtgg gcgattgcgc agcaaataga gagacgaaat gcgtgcatag
    17041 tcgaagtgca attgccgaaa aaacagcgtc tggttcgggt gattgaaaaa gggttaccgc
    17101 gtgacgcctg attcatccac agcttgtgaa taaaaataaa aacgcattgc aattcaagac
    17161 gaataaaatc gtcaagtgat cgactcgaca aaacggaatc ttgattttta acttatttat
    17221 caataggtta aaaaaatacc attcaccgtg cgaagtttct ttgaaattaa gattacagcc
    17281 tgaggctgtg gataactctg tttacaagag tttgtcagag acaacaaaaa ccagcaataa
    17341 cgcatcgcgt cgctgtagat attttattga gagaagaatt tggagcggga aacgagactc
    17401 gaactcgcga ccccgacctt ggcaaggtcg tgctctacca actgagctat tcccgc
//
LOCUS       DAJTQQ010000081        16948 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000081 DAJTQQ010000000
VERSION     DAJTQQ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16948
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.081"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            445..1011
                     /gene="mntP"
                     /locus_tag="OO309_002951"
     CDS             445..1011
                     /gene="mntP"
                     /locus_tag="OO309_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR5848019.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(1008..1817)
                     /gene="rlmA"
                     /locus_tag="OO309_002952"
     CDS             complement(1008..1817)
                     /gene="rlmA"
                     /locus_tag="OO309_002952"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR5848020.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1983..2192)
                     /gene="cspE"
                     /locus_tag="OO309_002953"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1983..2192)
                     /gene="cspE"
                     /locus_tag="OO309_002953"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5848021.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(2205..2348)
                     /locus_tag="OO309_002954"
     CDS             complement(2205..2348)
                     /locus_tag="OO309_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR5848022.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(3019..3306)
                     /locus_tag="OO309_002955"
     CDS             complement(3019..3306)
                     /locus_tag="OO309_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR5848023.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(3381..3524)
                     /gene="mgrB"
                     /locus_tag="OO309_002956"
     CDS             complement(3381..3524)
                     /gene="mgrB"
                     /locus_tag="OO309_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR5848024.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            3683..3922
                     /locus_tag="OO309_002957"
     CDS             3683..3922
                     /locus_tag="OO309_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848025.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            complement(4065..4856)
                     /gene="kdgR"
                     /locus_tag="OO309_002958"
     CDS             complement(4065..4856)
                     /gene="kdgR"
                     /locus_tag="OO309_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR5848026.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            5033..6406
                     /locus_tag="OO309_002959"
     CDS             5033..6406
                     /locus_tag="OO309_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5848027.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            complement(6452..7333)
                     /gene="htpX"
                     /locus_tag="OO309_002960"
     CDS             complement(6452..7333)
                     /gene="htpX"
                     /locus_tag="OO309_002960"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR5848028.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(7525..9573)
                     /gene="prc"
                     /locus_tag="OO309_002961"
     CDS             complement(7525..9573)
                     /gene="prc"
                     /locus_tag="OO309_002961"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR5848029.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(9593..10291)
                     /gene="proQ"
                     /locus_tag="OO309_002962"
     CDS             complement(9593..10291)
                     /gene="proQ"
                     /locus_tag="OO309_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR5848030.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(10388..10885)
                     /gene="msrC"
                     /locus_tag="OO309_002963"
     CDS             complement(10388..10885)
                     /gene="msrC"
                     /locus_tag="OO309_002963"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR5848031.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            11015..12298
                     /gene="yebS"
                     /locus_tag="OO309_002964"
     CDS             11015..12298
                     /gene="yebS"
                     /locus_tag="OO309_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR5848032.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            12267..14887
                     /gene="yebT"
                     /locus_tag="OO309_002965"
                     /pseudo
     CDS             12267..14887
                     /gene="yebT"
                     /locus_tag="OO309_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            14967..16406
                     /gene="rsmF"
                     /locus_tag="OO309_002966"
                     /pseudo
     CDS             14967..16406
                     /gene="rsmF"
                     /locus_tag="OO309_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            16524..16760
                     /locus_tag="OO309_002967"
     CDS             16524..16760
                     /locus_tag="OO309_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR5848033.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            16865..>16948
                     /locus_tag="OO309_002968"
     CDS             16865..>16948
                     /locus_tag="OO309_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HCR5848034.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQ"
BASE COUNT         4104 a         4277 c         4092 g         4475 t
ORIGIN      
        1 gtatcaactc aataacttat gaatttagcc aaagctatgt ttagtgtatt tttaataatc
       61 agacatagct taggctatat tatctcttcc cttatttgtt atttatttta acgtttcatt
      121 gatatataaa cataaatgaa aatcgttatc aataaagcaa tgaaataata tattccaaca
      181 gttgttttat attctcaaaa tatgttaagg ttgcgccctc atttggggag tagccgattt
      241 ccagattccg gaaatgtacg tgtcaacata ctcgttgcaa aacgtggcac gtacggactg
      301 aagactttca gtcaggcgag accatatgca catcaatcgc tatgcctgca tgaacgcgaa
      361 aacccatcgc ggcttgttgt tcatgtatgc atgtttgctg ggggcgatga tgtgttttat
      421 ggataccccg gtcaggacgt tgtcatgaat atcactgcta ctgttcttct tgcgtttggt
      481 atgtcgatgg atgcatttgc tgcatcaatc ggtaaaggtg cccccctcca taaaccgaaa
      541 ttttctgaag cattgcgaac cggccttatt tttggtgccg tcgaaaccct gacgccgctg
      601 atcggctggg gaatgggcat gttagccagc cggtttgtcc ttgaatggaa tcactggatt
      661 gcgtttgtgc tgctgatatt cctcggcggg cgaatgatta ttgagggttt tcgtggcgca
      721 gatgatgaag atgaagagcc gcgccgtcgg cacggtttct ggctactggt aaccaccgcg
      781 attgccacca gcctggatgc catggctgtg ggtgttggtc ttgctttcct tcaggtcaac
      841 attatcgcga ccgcattggc cattggttgt gcaaccttga ttatgtcgac attagggatg
      901 atggttggtc gctttatcgg ctcaattatt gggaaaaaag cggaaattct cggcgggctg
      961 gtgctgatcg gcatcggcgt ccagatcctc tggacgcact tccacggtta ataagaacgc
     1021 tgccagaggt gaatattaaa gtccgtctgg cagtcgaaca cttcttttgc tgccagtgtt
     1081 tgccagactt ctggcttcgc acgccaggca aacggcgtca tctgcaataa tgcgacggct
     1141 tcatcaccgc gaagacgcat cggataacac aactccgcac tctgctgtaa tgtaaaacct
     1201 tccagttgtt ctgcatgagg tgcatgaaga tgtacttcat tgtaaatcag ccccttcagc
     1261 tccatcaaat gtcgcggtcc cggcgtggca gtaatgaccc agccgccggg cttcactact
     1321 cgtgctaatt cttctgcttt acacggcgcg taaatacgta ttatggcgtc catactggta
     1381 tcggaaaacg gcaaacggtg gctggaagcg acacaaagag tgacctgcgg atagcgtttc
     1441 gccgccgctt ttatcgctac cttcgagaca tccaggccaa acgtggtgat ttcgggcaac
     1501 gcatcggcaa atgcgtgtgt gtaataccct tcaccacagc caatatccag caccgccgtg
     1561 gccttttcat caagccgttc ccgcagttgg gcgacaattg catcacgcag cggctgataa
     1621 tgtccggcat ctaagaatgc gcggcgtgct tgcatcattt ccgcgctgtc gcccggatca
     1681 cgagaccgtt tatgctgaac gggcagcaga ttgacatacc cttctttcgc catatcaaac
     1741 tgatgtcgtt ggggacagat atagctgttt ttttcacgcg aaagaggctg atggcaaagt
     1801 ggacaggaaa acgacatgac aactccggca ggatactaaa ggccgcaagt gtaacgcgaa
     1861 tcacgcggcg ggcaaatatc cgaacgccag gagcgaagat aaagcatagt aaaaagcctc
     1921 gcattcgacg aggctttata gcgagattga agcgtattca cacttcagat cagtggattc
     1981 gatcagatag ctgttacgtt aacagctgcc ggacctttct ggccgtcctg aatttcgaac
     2041 tcaacgttct gaccttcagc cagagttttg aagccattac cctggatagc ggagaagtgt
     2101 acgaacacat ctttgctgcc atcagccgga gtaatgaagc caaaaccttt agactcgttg
     2161 aaccacttaa cctgaccttt aatctttgcc atttgaaaaa ttccttagat tgttttcttc
     2221 gcccgcaggc ataacataga taaaactgac acattactgc atgaggcacc aatataaggc
     2281 tcggcagaga agcggtattc aacgtcaacg tgtttactca ggacttcttt actgaaaatg
     2341 ccacacataa acagaactgt acctcgttta acccgaaatc ttttatcaca tacaacgtta
     2401 attatggcaa gccattttta aacatatctc gatcagacgc acaaatctcg atacgtcttc
     2461 cacttttttt gcacacttat gcaacggaat acgcgccgat atatcattgt gcttaacctt
     2521 gccagttcag gcagatactt aaactggcgt agtttctaac atagttcaat aagttcagtt
     2581 gttccaggcc agagggagaa gttattacat agtgcgtgca atatcacatt ttctgctatg
     2641 caatgaataa aaagttatat cactttttct cataaaacag tcagttaacg gctatcaatt
     2701 accctaaaga gaagtcaatc cccaaaggga ttgtaaattt aaaataagaa aaattgatga
     2761 gtgagcaaaa aaaatcaaga gagaaacgtt tcgctaataa ataataccac gcttaatcac
     2821 aaatctgatg taaacatcct aataaattaa tgaggaaaat atctttttct gatatcccgt
     2881 tacgcaaata aataatatga taattatttg aagtcagacc agagttttaa tttgaagcag
     2941 ttgcaccatg acagtgcgta taatcaggat gaattgagta acccttcaac gacggggtta
     3001 ctcaagattg aggaaggatt atcgttcggc gaccaggcga ataatatctt cgtcatcaac
     3061 cgatttttga ctctcaatgg tctttttatc tgctttctct ggttcgtttg cttcgcacaa
     3121 acgacgtaac agtgcatttt gacgtttttg ctgatccagc aacgcctcaa gcagttcaat
     3181 ctgttcgtta gtccgtgaac tggcacgatt gataaaaaac cacaagatga gcccgataag
     3241 aagaaccacc accgatacaa ccaaagacgc aatgttcatc acgcctgaat ttacaacttc
     3301 gttcatttca ccacctctga gtagaggcgc tattctacca ctgctggaga ggaagaaaat
     3361 ctagtgctga aaaaatgata tcaccacggg ataaactggt taatggcaca aattccgctg
     3421 aaaaattgta catcctgatc gcacatcatg ttgaatacct gcgcccaaag cagcaagcaa
     3481 gccaacacca cgacaaccag aacgacccat cgaaactttt tcactccaat ctccgcttca
     3541 cctaccttat gtcattaaac tagcatgcgt tagttaaaca gcgcctaact atgtcgatat
     3601 tcgtgctttt tcggaatgag tagcttgctt cagcgcatat tgccgctacg attaagcgaa
     3661 cataaaaaga gaagagattg taatgcgatt catcattcgc acagttatgc tgatagcact
     3721 ggtatggata ggattattac tcagcggata tggcgttttg attggaagca aagagaatgc
     3781 cgcaggatta ggtttgcaat gtacctatct gactgccaga ggcaccagca cggtacaata
     3841 cttgcacact aaaagtggtt tcttggagat aacagattgc ccactgttac gtaaaagcaa
     3901 tatcgtggtc gataatggtt aagggtaata aataaccaga ttctcactca tgagtaaaat
     3961 attcttatag caagcgcgtt taatataacg caccttcaac tttatttttc tcatgctaaa
     4021 taaagaacct gtctgtttga tattccgaca ggttcttact ctcatcagaa cggatagtcg
     4081 tgataaccca tttgggcaga aattttgcgc gctgcggtat gcaacattgc gacatattcc
     4141 tgtaaacgct cttcagagaa acgcaacgtc gggaaggaga tgctcaaacc ggcaatgacc
     4201 acgccaaagc gatcaaatac cggtaccgca atgcattgca gcccttcttc ctgctcttcg
     4261 ttatcttctc catacccctg ctcgcgcacc tggtccagaa cgggtaataa cgcttctgta
     4321 ctggtgatgg tccgctcggt actgcgttta tactccacgc cctcaagaat ttgcttcact
     4381 tcatcgcgat cgcgccatgc cagcagtacc ttaccaatcg cggtgctgta cagcggatta
     4441 cgacgcccaa tccgtgaata catgcgcaaa ttgtacatag agtcaatttt gtgaatgtaa
     4501 acaatactgt cttcgtccag tgcgccgagg tggatagttt ctttggtcag gcgggagatc
     4561 tcacgcatct ggatatctgc gctacgaatt aaatcgacgt tttgtaacgc gcgagcgccc
     4621 agttcaaaca atttcagggt cagggaatat ttctccgact ccccttcctg cgccacataa
     4681 cctaaggttt tcatggtctg taaaaagcga taaacggtgc tttttgacat catgacgcgc
     4741 tgcgacagct cggttatccc tatttcgcgc tcttcaccca gcgcctgcaa aatgccaaaa
     4801 acttttagca cggaagatac agaatcaggc tgtttatcca gatctgcgtt agccatttat
     4861 cacctcattg cgagtgtttt ataaaaatca gaactgtttt ttattataat ttcgcaccag
     4921 ggtggtcgca atccatcttt tgccggttag ttacaattct gcgacatcca ccgtgaatat
     4981 cagtgctaga atcatacccc tgttgattat tcaccaaaga tataaaattc ctatgccaaa
     5041 agttcaggcc gacggcctgc cattgcccca gcgatacggt gcgatattaa ccattgtgat
     5101 tggtatttcg atggctgtcc ttgacggcgc aatcgccaac gtcgccctgc caacaattgc
     5161 cacggacctt catgccacac cagccagttc catctgggta gtgaacgcct atcaaatcgc
     5221 cattgtcatc tccctgctct cgttttcgtt tctgggcgat atgtttggct atcgacgtat
     5281 ttataaatgc ggtctggtcg tttttctgtt gtcttcactg ttctgcgccc tttctgattc
     5341 gctgcaaatg ctcacccttg cgcgtgtcat acaaggtttc ggcggtgcag cgttgatgag
     5401 cgttaatacc gcacttatcc gcctgatcta tccacaacgt tttctgggga gagggatggg
     5461 cataaactcg tttattgttg ccgtctcttc tgctgccggg ccgacaattg ctgcagcaat
     5521 cctctccatc gcatcctgga aatggttatt tttaatcaac gtaccgttag gtattatcgc
     5581 cctgcttctg gcgatgcgtt ttctgccacc caatggttct cgcgccagta aaccccgttt
     5641 cgacctgccc agcgccgtga tgaacgcgtt aaccttcggc ctgcttatca ctgcgttgag
     5701 tggtttcgct caggggcaat cgctgacgtt aattgctgcg gaactggtgg taatggttgt
     5761 tgttggtatt ttctttattc gccgccagct ttctcttccc gtaccgctgc taccggtgga
     5821 tttactgcgt atcccgctgt tttcactttc tatttgcaca tctgtttgct ctttctgcgc
     5881 acaaatgctg gcaatggttt ccctgccctt ttacctgcaa accgtgctcg ggcgtagtga
     5941 agtcgaaaca ggtttacttc tgacaccgtg gccgttagca acgatggtga tggctcctct
     6001 ggcaggctat ttgattgaac gcgtacatgc aggattgctg ggtgctttag ggttgttcat
     6061 catggctacg gggctttttt ccctggttct gctgcccgcg tcacctgcgg atatcaatat
     6121 tatctggccg atgatcttat gtggcgctgg atttggctta ttccagtcac ccaataacca
     6181 caccattatt acctccgctc cgcgcgaacg tagcggtggg gccagtggca tgttaggaac
     6241 ggctcgtcta ctgggtcaga gtagtggcgc ggcgctggtg gcgctgatgc taaatcagtt
     6301 tggtgataat ggtacgcacg tctcgctgat ggctgcggct attctggcag tgattgctgc
     6361 ctgtgtcagt ggtttacgta tcactcagcc acgagccagg gcataaaaaa agcgcgtcga
     6421 tcaggacgcg ctttttagta tttacttcat attacttcag gtattcaccc gtacgcagag
     6481 cttcaatacg tttatccagc ggcgggtgag tcatgaacaa ctcactgagc gatttcgact
     6541 taccgttaat gcagaaagcc atcatgctgg ttgcttcttg cggttcatag ctggttttca
     6601 ggcgctgcag cgcggcaatc attttctcgc gaccaaccag ttttgccgaa ccggcatctg
     6661 catggaattc acgatgacgc gagaaccaca tggtgataat gctcgccaga ataccaaaca
     6721 ccagttccag aaccgttgca accgcaaagt agatcagcgg gttgccgttg ctctcttcac
     6781 cttcatcacg atttccgccc ataaaacccg cggcaagctg cgccagaata cgggaaataa
     6841 agataacgaa ggtgttcacc acgccctgaa tcagcgtcat ggtgaccata tcaccattgg
     6901 cgatgtggct gatttcgtga gcaattaccg cctcggcttc atccgggctc atgttctgca
     6961 gcaaaccggt gctgacagca accagagagg catcacggcg cgcaccggtt gcaaaagcgt
     7021 tgatgtccgg cgcatggtag atagccactt gcggcatagc gatccccgcc tgacgagcct
     7081 gggttgctac agtattgacc agccaacgtt ccctttcgtt acgcggttgc tcgatcactt
     7141 ccccgccaac agatcgtaat gccatccatt tggacatcag aagcgaaacg aaggaaccac
     7201 caaaaccgaa cagcaaggcc atgatcatca gcccctgaac gctgctcgac tgtatccctg
     7261 tcaggctcag taccagcccg aaaacgacca ttacggccag gttcgttagc aggaagagcg
     7321 cgattcgcat cataattttc tttttacctc agtttaacaa aacgcaatat gcgataccca
     7381 catcgtatgg gttacgcgac tattttcaag tctggatagt gcgtaagtca ccagaaagac
     7441 acaactttac attttgtagc atctgattta cggcatcttg ccgctgttaa aaaatcaggc
     7501 acaatttctt gtgcctgatt gatattactt gacgggagcg ggttgttccg cgggtctggc
     7561 tttttcaagc ttcgccagat cgagtgcgat attcaccgtc tcatccagat aaggatccgg
     7621 ctcctggtaa tctttcgata gatcatccag cttcttcaac tccggtttac cttctcgttt
     7681 aaagcgttcg ttcaaacgcg ccagacgcgt cgcatcatct tcattattct ctttctcacg
     7741 cacagcgtag ttcagagaaa cgatattgcg cttgtccttc atagcgttga agcgcgcgat
     7801 atccttcatg atgttctgga actcaggatc tttcgcgata cgcgcattat gttccttcag
     7861 cagctccggt tcaaaggcag ttaaatctcc tgatttcaca taagtcgcgg catcgatgct
     7921 atcccacggc agcgcgttat cttcgaattt ctcacccgtt tccgtttctt cattacccgt
     7981 cggcatgatg atgtctggtg ttacgccttt acgttgcgta ctgccgccgt taacgcgata
     8041 gaatttctgg atcgtgtact gcacagaacc cagcgctggc cattcaggac gtaacatctg
     8101 atcgtaaata cggttcaatg aacggtattg ctgaacggtg cctttaccaa acgtcggttc
     8161 acccacaacc agcgcacgac cgtaatcctg cattgccgcg gcaaagattt ctgaagccga
     8221 agcactgaaa cggtcaacca gtaccaccag cgggccttta tagaacacct gcccgtcggt
     8281 atcgctgtct tcacgaacct tgccgttgtt atcgcggacc tgaacaatgg gacccgcagg
     8341 aataaacaga cctgagagcg ataccgcttc ggtcaacgcc ccaccgccat tgctacgcag
     8401 gtcgatgatc acactgctga cattctgttt ttccagtttc tgcagttgaa ctttgacatc
     8461 gtctgtcaaa cccacataga agcccggaat atccagcacg ccgactttct ctttaccgac
     8521 ggtcttcacc gacattttaa ccgcgcggtc ttcgagacga atacgttcac gggtcaacgt
     8581 tacagtacgg gtcttggtcc ctttaccagc aggtaaaatt tccagacgaa ctttactgcc
     8641 cttcggccct ttaattaagg caaccacatc atcaagacgc cagccaatca cgtcaaccat
     8701 cggcttgcct gtttgaccaa caccgacaat tttgtcacca acgctgatag ctttactctt
     8761 cgctgccgga ccacctgcca ccatcgaatt gataacggtg tagtcatcat ccatttgcag
     8821 cactgcgcca ataccttcca gtgacaaact catttcagtg ttgaactgtt cggtattacg
     8881 cggggaaaga tagttggtat gcggatcgat ttcacgcgca aacgccgtca ttgccagcga
     8941 gaaaacatct tcgctgttgg tttgcgccag acgacgaatg gcaaatttgt agcggcgtgt
     9001 cagggtttca cgaatttctt tatccgtttt tcctgtcagc ttcaggctta actcgtcgaa
     9061 tttgacttta ctgtcccact gcgcgttcaa ctcagcctcg tttttcggcc agggcgcttt
     9121 gctgcggtca aggttataag tgtcgttgcc ggtgaaatcc atcggctttt ccagtaccga
     9181 caaagcgtac tggtaacgtt caaaacggcg cttttgcgcc agattgtaga gatcgtagaa
     9241 aacgtcgagt ttgcctgaac gcagttcatc gcctaactcg gttttctttt tcgcgaactg
     9301 ttcaacatcg cttgccagca gcacattgtg gctgtaatcg agcagattca ggtagcggtc
     9361 aaagattttg gccgaaaatg cctgatcgag gtcgaactgg cgataatgag aacgggtgaa
     9421 gcgcgacgtt acgcgctcac ttaccgtcgc atgctgcgtc tcttccttta ataccggaat
     9481 ttgatcagca cgcgtgatat cttctacagc gaaggtctgg cctgctattg caagcaggcc
     9541 agctaacgcg gtaagcctaa aaaacatgtt catgcctggc ccggcctccg tttcagaaca
     9601 ccaggtgttc tgcgcgcaca atcaaagaca tacccgaatt cagctggacg cggacgccgt
     9661 ctttggtgat ttctaatacg gtggcatcca tcgcgttttg acccgctttc accttcaggg
     9721 cttgtccgac agtcagagct gaaatgtcag aaaccggggt gtgctgttct tcgcgaggtg
     9781 cttttactgt ttttggcgct ttctctaccg gcttttgcgc acgaggttta cgttcagcgc
     9841 cttctttgcg gcgtggcgtg gtcggacgtg gcttgcgttc gcggcgcggt gcgtcttctt
     9901 tctcaccagc agctgcggca gcttcgcgtt ttttcgcttg ctgttcagca cgctgtgcct
     9961 gaacacgcgc tttcgcttct tcaagctgct tgcgagcatg ctctacatgt tgctcgtcca
    10021 gctcaccgca tgggttgccg tcaagatcga cacgcgttgc gccgggttta acaccgtaaa
    10081 gataacgcca gctcgaagtg tagagacgta aagcggatcg taattgcgtt ttgctcaggt
    10141 tcatttcccc ggcaacacga tcgaccaaat cctgaaaaat accaattttc agcggacgcg
    10201 cttcaccttc cgcactgaaa cagtggggaa aacgttcggc cagaaacgcg attacttctt
    10261 tactgctatt caacttaggt tgattttcca tgaaatttcc tgattacaac ggacgtagcc
    10321 aacaagcgca ggcatgaaca ggcgtcatta taatgacgct accagtaaat gctacgttat
    10381 ccgttgatta tcctgcgacg ctcgcaaaga attttttgta atccgtcgtt gcaagcactt
    10441 tttcaagctg tgccacaagc tgacgtaagc cttgctcgtc ctcgtctgta aagcgagcga
    10501 agacagtact atcgatgtcg agaacaccaa taatctgatt tttcaccacc agcggcagaa
    10561 caatttcaga attactcgcc gcatcacagg caatatgccc gtcaaacgcg tgaacatcct
    10621 cgatacgctg cacttgattg cgggcaaccg ctgtgccgca caccccgcgc ccgacgggta
    10681 tccggacaca ggcaattttg ccctgaaatg gtccgagtac cagtgtatcg tcctcaagca
    10741 aataaaaacc tgcccagtta acgtcagtga gacgctcgta taacaacgca ctggtgttcg
    10801 caagcgttgc cagaaaactg gtttctcccg ccatcagcgc gttaaagtcg cgatttaaat
    10861 ccgcgtaaaa ttctgttttg ttcattactt aatcacttgg ttgtcttacc tggaactgcc
    10921 agcctattaa aataagcatt aaatgcgtta atgctcaaga tcattcccat catgggttaa
    10981 gattaatgtt aattcttatt acatttggca cgtcatggct cttaacacac cacaaattac
    11041 gccgacaaaa aagataacag tgagggcaat cggcgaggaa ctgccgcgtg gtgattacca
    11101 acgttgcccg caatgtgaca tgctgtttag cctgcccgag ataaattctc atcaaagtgc
    11161 ctattgtccg cgctgtcagg caaaaattcg cgacgggcgc gactggtcgc taacgcgcct
    11221 ggcggcaatg gccttcacca tgctgttgct tatgccgttt gcctggggcg aaccgctgtt
    11281 gcatatctgg ctgttaggca ttcgtattga cgccaacgtc atgcaaggca tctggcaaat
    11341 gaccaaacag ggcgatgcga taacgggatc aatggtcttt ttctgcgtta ttggtgcccc
    11401 cctcattctg gtgtcctcca tagcatattt atggtttggt aaccgactgg gaatgaattt
    11461 acgtccggta ctgctgatgc ttgagcgact taaagagtgg gtaatgctgg atatctacct
    11521 ggtcggcatt ggcgttgctt ctataaaggt acaggattat gcccatatcc aggcgggtgt
    11581 cggcttgttc tcttttgtgg cgttggtgat tttaacgacg gtgacgttgt cacatcttaa
    11641 tgttgaggag ctgtgggagc gatattatcc gcagcgcccc gctacgcgta gggacgagaa
    11701 acttcgtgtc tgtcttgggt gccattttac cggctatcct gatcagcgtg gtctctgccc
    11761 gcgttgccat atcccgctac gcctgcgtcg tcgtcatagt ttgcaaaaat gctgggcggc
    11821 gctgttagcg tcaatcgttt tgttgttacc agccaaccta ttgcctattt ctatcattta
    11881 tctgaatggt ggccggcagg aagatacgat tctttccgga attatgtcgc tggcaagtag
    11941 caacattgcg gttgcgggaa tcgtgtttat cgccagtatt ctggtaccgt ttactaaagt
    12001 gatcgtcatg ttcactttac tgttgagcat tcattttaaa tgccagcaag gtttacgcac
    12061 acgcattctg ttactgcgga tggtgacctg gattggacgc tggtcgatac tcgacctgtt
    12121 tgtcatatct ttaaccatgt cgctgattaa tcgcgatcag atcctcgctt ttactatggg
    12181 accggctgcg ttttattttg gcgctgccgt aattttgact attcttgctg tggaatggct
    12241 ggacagccgc ttactttggg atgcacatga gtcaggaaac gcccgcttcg acgactgaag
    12301 cgcagattaa aaataaacgc cgtatctcac ctttctggct gctgcctttc atcgcgctaa
    12361 tgattgccgg ttggctgatt tgggacagtt atcaggaccg gggtaatacc gtcaccatcg
    12421 actttatgtc ggcggatggt attgttccgg gccgtacgcc tgttcgttat cagggcgttg
    12481 aagtcggaac agtgcaggat atcagcctca gcgacgatct tcgtaagatt gaagtcaagg
    12541 tcagcatcaa gtccgatatg aaagatgcgc tgcgcgaaga gactcagttc tggctgatga
    12601 cgccaaaagc atcgttggca ggtgtctccg ggctggacgc cctcgtcggt gggaactata
    12661 tcggcatgat gccgggtaaa ggtaaagagc aggatcactt tgtcgcgctc gatacccaac
    12721 cgaaatatcg gctggacaat ggcgatctga tgatccacct gcaagccccc gctctcggtt
    12781 cgctgaacag cggttcattg gtctatttcc gcaagatccc ggtgggaaaa gtctacgact
    12841 atgccatcaa tcccaacaag caaggcgtgg tgattgatgt cctgatcgag cggcgtttta
    12901 ccgatctggt gaaaaaaggt agccgtttct gaaacgtttc cggcgttgat gccaacgtca
    12961 gtatcagtgg cgcgaaggtg aaactggaaa gtctggcggc actggttaac ggtgcgattg
    13021 ccttcgattc acctgaagag tcgaaacctg ccgaggcgga agataccttt ggtctgtatg
    13081 aagatctggc ccacagccag cgtggagtaa taataaaact ggaactgccg agtggagcag
    13141 gattaaccgc cgactcgacg ccgttaatgt atcaggggct ggaagtcgga cagctgacta
    13201 aactggattt aaatcctggt ggtaaagtca ctggggaaat gaccgttgat ccctgcttcg
    13261 tgaaaatacc cgcatcgaat tacgcaaccc gaaattatcc cttagcgatg ctaatctcag
    13321 cgccctgctg accggaaaaa cctttgagtt ggtgcccggc gatggcgagc cacgcaaaga
    13381 gttcgttgtt gtgccgggcg aaaaagccct gctgcaagag ccggacgttc tgacactgac
    13441 cctgacagca ccggaaagtt acggtattga tgcgggtcag ccgctcattc ttcacggcgt
    13501 gcaggtaggc caggttatcg atcgtaaact caccagcaaa ggcgtcacct ttaccgtcgc
    13561 catcgagcct cagcatcgag aactggtaaa aggcgatagc aaatttgtcg tcaacagccg
    13621 tgtcgacgtg aaggtggggc tggatggcgt tgagtttctc ggtgccagcg cctcagaatg
    13681 gattaacggc gggatacgta ttctgccggg cgataaaggc gagatgaaag ccagctatcc
    13741 actgtatgcc aatctggaaa aagcgctgga gaacagcctt agcgatttac ccaccacaac
    13801 catgagtttg agtgcagaga cgctgccgga tgtgcaggca ggatcggtag tgctgtaccg
    13861 taaatttgaa gttggtgaag ttattaccgt gcgtccgcga gctaacgcgt tcgatatcga
    13921 tctgcacatt aaaccggaat atcgcaacct tttgaccagc aacagcgtgt tctgggcaga
    13981 aggcggggcg aaagttcagc tgaatggtag tggcctgacc gtacaggcat ccccgctctc
    14041 cagagcatta aagggggcca ttagcttcga taacctcagt ggtgccagcg ccagtcagcg
    14101 taaaggcgac aagcgaattc tgtatgcttc cgaaacagcg gcccgtgcgg ttggcaggca
    14161 gattacgctt cacgctttcg atgccggaaa actggcggtc gggatgccaa ttcgctatct
    14221 cggtattgat atcgggcaaa tccagacgct ggatctgatt accgcacgca atgaagtaca
    14281 ggcaaaggcg gtgctctatc cggaatatgt ccagaccttt gctcgcggtg gtacgcgctt
    14341 ctcagtggtc acaccgcaaa tctcggcagc tggcgttgag catctagata ctatcctcca
    14401 gccgtatatc aacgtcgaac caggccgtgg caatcctcgt cgcgactttg aattagaaga
    14461 ggccaccatt actgattcgc gttacctaga tggtttaagc attattgttg aagcgccgga
    14521 agccggttcg ttaggcatcg gtacgcctgt gctgttccgt ggtctggaag taggtacggt
    14581 taccggaatg acgctgggga cattgtcaga tcgcgtgatg attgcgatgc gcatcagtaa
    14641 acgctatcaa cacctggtgc gtaacaattc cgtcttctgg ttggcatcgg gttacagtct
    14701 ggactttggt ctgacgggcg gcgtagtgaa aaccggcacc tttaaccaat ttatccgtgg
    14761 cggcatcgcc ttcgccacgc ctccgggtac gccacttgca ccgaaagccc aagaaggcaa
    14821 acacttcctg ttgcaggaaa gtgaaccgaa agagtggcgt gaatggggaa ctgcgcttcc
    14881 caaataatgc ccactgctcc ggcgtgcctg cgccggagcg cttatgctaa actgcgcgcc
    14941 tgtttttttg ccagtggtac atgctcgtgg cccaacacac cgtttatttc ccggacgcgt
    15001 ttctgacaca aatgcgcgaa gctatgcctt cgacgctctc atttgatgat tttcttgccg
    15061 cctgtcagcg cccgttgcgc cgcagcattc gcgttaatac gctgaaaatc tccgttgctg
    15121 atttcctgca attaaccgct ccttatggct ggacgcttac gccaattccg tggtgtgaag
    15181 aaggtttctg gattgaacgc gacaatgaag atgcattgcc attgggtagt accaccgagc
    15241 atttaagtgg cctgttttat attcaggaag ccagttcaat gttgcccgtc gccgccttgt
    15301 ttgctgacgg taatgcacca cagcgggtga tggatgtcgc tgctgcgcca ggctcaaaaa
    15361 cgacgcaaat tgccgcgcgg atgaataacg aaggggcaat ccttgccaat gagttttccg
    15421 ccagttgggt aaaagtgtta catgccaata tcagccgctg tggcatcagt aatgttgcgc
    15481 tcacacattt tggtggccgc gtgtttggtg cggcagtgcc agaaatgttt gatgccattt
    15541 tgctggacgc tccctgctcc ggcgaaggcg tggtgcgtaa agatcccgat gcgctaaaaa
    15601 actggtcacc cgaaagcaat caggaaatcg cagctaccca acgggaactg attgacagcg
    15661 cctttcatgc attacgtcct ggcggtacgc tggtttactc gacctatacc ttaaaccggg
    15721 aagaaaacga agccgtttgc cggtggctga aagagactta ccccgacgaa gtagagtttt
    15781 taccacttgg cgatctcttc cctggtgcaa acaaggcgct gaccgaagaa ggttttttgc
    15841 atgttttccc acaaatttac gactgcgaag gcttcttcgt tgctcgtctg cgtaaaactc
    15901 aggcgatccc cgccttaccc gccccaaaat acaaagtggg caatttcccg ttcagcccgg
    15961 tgaaagatcg cgaagccgga caaattcgtc aggcggctgc aagtgttggc ttaaactggg
    16021 atggaaacct gcgactctgg caacgcgaca aagaactgtg gttgttcccg gtaggcattg
    16081 aagccctgat cggtaaagtc cgattttctc gcttagggat taaacttgcc gaaacgcata
    16141 acaaaggtta tcgctggcag catgaagcgg ttattgccca tgcctccccc gacaatgtaa
    16201 acgcttttga actgacaccg caggaagcgt aggagtggta tcgcgggcgc gatgtttacc
    16261 cgcaagccgc gccagtggcg gatgacgtgt tggttacttt ccagcatcag ccgattggtt
    16321 tagccaaacg gattggttcg cgactgaaaa acagctaccc gcgtgaactg gtgcgggacg
    16381 ggaaactttt taccagtaac gcatgacagc gcacaaaaaa agcgcacttt ttgactggca
    16441 cattcgccgg cctcaactag gctgaaaaat tgtgcgatcg gactggtcgt actacaatca
    16501 gcagctaaat ggagagcacg aagatgaaaa ccagtgtgcg cataggcgct tttgaaatcg
    16561 acgacggcga attacacggt gaatcgccgg gtgatcgaac gttaaccatt ccttgtaaat
    16621 ctgaccccga tttatgtatg caactggatg cctgggatgc tgaaaccagt atccctgccc
    16681 tgctaaatgg cgaacactct gtcctttacc gtacccgtta cgatcaacaa tctgatgcct
    16741 ggattatgcg tcttgcctga tccaaaaaga acccgtcggc atggcgggtt atttgtcctg
    16801 gttattcccc cgttgtaaaa tctctcctaa acttaacggt acggcaccac acttcgggga
    16861 tgaaatgttc gcgctggtac tttttgtttg ctacctggat ggcggttgtg aagatattgt
    16921 tgtggatgtc tacaacacgg aacagcag
//
LOCUS       DAJTQQ010000082        16825 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000082 DAJTQQ010000000
VERSION     DAJTQQ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16825
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.082"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..510
                     /locus_tag="OO309_002969"
     CDS             <1..510
                     /locus_tag="OO309_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCR5848035.1"
                     /translation="PFREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDG
                     FTQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATI
                     RHNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADR
                     QYSRAWTVK"
     gene            complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="OO309_002970"
     CDS             complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="OO309_002970"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCR5848036.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1780..2868
                     /gene="hcxA"
                     /locus_tag="OO309_002971"
     CDS             1780..2868
                     /gene="hcxA"
                     /locus_tag="OO309_002971"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR5848037.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2878..3075)
                     /locus_tag="OO309_002972"
     CDS             complement(2878..3075)
                     /locus_tag="OO309_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5848038.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="OO309_002973"
     CDS             complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="OO309_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR5848039.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5543..5956)
                     /gene="entH"
                     /locus_tag="OO309_002974"
     CDS             complement(5543..5956)
                     /gene="entH"
                     /locus_tag="OO309_002974"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR5848040.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5959..6705)
                     /gene="entA"
                     /locus_tag="OO309_002975"
     CDS             complement(5959..6705)
                     /gene="entA"
                     /locus_tag="OO309_002975"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR5848041.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6705..7562)
                     /gene="entB"
                     /locus_tag="OO309_002976"
     CDS             complement(6705..7562)
                     /gene="entB"
                     /locus_tag="OO309_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR5848042.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7576..9186)
                     /gene="entE"
                     /locus_tag="OO309_002977"
     CDS             complement(7576..9186)
                     /gene="entE"
                     /locus_tag="OO309_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR5848043.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9196..10371)
                     /gene="entC"
                     /locus_tag="OO309_002978"
                     /pseudo
     CDS             complement(9196..10371)
                     /gene="entC"
                     /locus_tag="OO309_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10663..11619
                     /gene="fepB"
                     /locus_tag="OO309_002979"
     CDS             10663..11619
                     /gene="fepB"
                     /locus_tag="OO309_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR5848044.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11623..12855)
                     /gene="entS"
                     /locus_tag="OO309_002980"
     CDS             complement(11623..12855)
                     /gene="entS"
                     /locus_tag="OO309_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR5848045.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12966..13970
                     /gene="fepD"
                     /locus_tag="OO309_002981"
     CDS             12966..13970
                     /gene="fepD"
                     /locus_tag="OO309_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR5848046.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13967..14959
                     /gene="fepG"
                     /locus_tag="OO309_002982"
     CDS             13967..14959
                     /gene="fepG"
                     /locus_tag="OO309_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR5848047.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14956..15771
                     /gene="fepC"
                     /locus_tag="OO309_002983"
     CDS             14956..15771
                     /gene="fepC"
                     /locus_tag="OO309_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5848048.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15768..16825)
                     /gene="fepE"
                     /locus_tag="OO309_002984"
                     /pseudo
     CDS             complement(15768..16825)
                     /gene="fepE"
                     /locus_tag="OO309_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
BASE COUNT         3899 a         4812 c         4398 g         3716 t
ORIGIN      
        1 ccctttcgtg aaaagccagt ggattgcgtt ctgtgggtta aaaacagcca acttatgcct
       61 aatgattaca acccaaacaa cgtggcacca cccgaaaaaa aactgctgca aaaatccatt
      121 gaaatagatg gttttaccca acccattgtg gtcacacata cggataaaaa tgccatggaa
      181 atcgtggatg gttttcaccg ccatgaaatt ggtaaaggct catcatcatt aaaattacgc
      241 ttaaaaggtt atttgccggt gacctgtctg gaaggtacgc gtaatcaacg catcgccgcg
      301 actattcgtc ataatcgcgc tcgtggacgc catcaaatca ccgccatgtc ggaaatcgtc
      361 cgtgaactta gccagttggg atgggatgat aataaaattg gcaaagagct gggaatggac
      421 agcgacgaag tgctgcgact gaagcaaatt aacggcctgc aagagttgtt tgccgaccgt
      481 caatattccc gcgcctggac ggttaaatag ctaaagctgg cgcaggcgtt cagctgctgc
      541 cagcaacgtc gattccttct tggcaaaaca gagacgaatc agtttatgtg ggaagggatc
      601 ggcgcaaaac accgacagcg gaatcgccgc tacgccgtgc tcctgcgtca gccactggca
      661 aaactcaaca tcatccaggg tagaaaccgc gctgtaatcc accagcaaaa agtatgtacc
      721 ttcacacggt aaaatctcca gccggctttc atttaaagca ttcaccagaa tatcgcgctt
      781 ctggcgataa aagtccggta acgcaagata atgctcaggt tctgcacgta gcatatcagc
      841 aagcgccagc tgtgccgggg tattcaccga aaaggtcaga tactgatgta ccttgcgaat
      901 ttcggcgctg atgggcgctg gcgcaacaca ataacccact ttccagccgg tcatatgata
      961 ggtcttgcca aatgaagaaa ccgccactgc ccgctcacgc agctgcggat gcgccagcac
     1021 actggcatgg ccctgttgtg aaaagttgat gtgctcgtag acttcatcgc taatgacaaa
     1081 aatctcgtgc ccggcgatcg cctgccacaa agcggcgaaa tcagcctgct gccagacagt
     1141 tgcactgggg ttatgcggag tgttgaggat caccagtctg gtgcgctcgc ttaataatgc
     1201 ggcaaattcc tgccagtcaa cgcgaaaatg cggtggttgc agtgccatac gtttcactat
     1261 tcccccagaa agcgcgatgg cgggggcgta actgtcatag ctgggatcaa aacaaatcac
     1321 ttcatcgcca ttgcgcacca gtgcggtaat cgccgcgtat aacgcttccg tcgcccctgc
     1381 cgttacggtg atatcgctat cggcatctgg ttgatagcca tacaaacgtt ccgttttctg
     1441 agcaatcgcc tcgcgcaagg cctgcacgcc ggtcatgggc gcgtattggt ttgccccctg
     1501 ggcaacgtgg tacgccagcc gctcctgtaa atagcgcgga ccatcaaaat caggaaagcc
     1561 ttgcgacagg ttaatcgcct ggtgttgctg cgccagcgcg ctcatctggg tgaaaatagt
     1621 ggtgccaagt tgtggaagtt tgctttgtgg aatcagaggg ttatttgtca tttttattgt
     1681 acctgacgaa gttgcgtgtt gaaggcacta taacacgatg ccagtatttg gcaatcaaga
     1741 cgtttagatg tctaaataaa acaataagga caacacaaca tgcctcacaa tcctatccgc
     1801 gtggtcgtcg gcccggctaa ctacttttca catccaggaa gtttcaatca cctgcacaat
     1861 tttttcactg atgaacaact ttctcgcgcg gtgtggatct acggcaaacg cgccattgct
     1921 gcggcgcaaa ccaaacttcc gccagcgttt ggacttccag gggcaaagca tattttgttt
     1981 cgtggtcatt gcagcgaaag cgatgtacag caactggcgg ctgagtccgg tgacgatcgc
     2041 agcgtggtga ttggcgtcgg cggcggcgca ctgctcgaca ccgcgaaagc cctcgcccgc
     2101 cgtctcggtc tgccatttgt tgccgctccg acgatcgccg ccacctgcgc agcctggaca
     2161 ccgctctccg tctggtataa cgatgccgga caggcgctgc attatgagat tttcgacgac
     2221 gccaatttta tggtgctggt ggaaccggag attatcctca atgcaccgca acaatatctg
     2281 ctggcgggga tcggtgacac gctggcgaaa tggtatgaag cggtggtgct ggctccgcaa
     2341 ccagaaacgt tgccgctaac cgtgcggctg gggatcaata atgcgcaggc cattcgcgac
     2401 gtcttgttaa acagtagcga acaggcactt gccgatcagc aaaatcagca gttaacgcaa
     2461 tcattttgcg atgtggtgga tgctattatt gctggtggtg ggatggtcgg tggtctgggc
     2521 gatcgtttta cgcgtgtggc ggcggctcat gccgtgcata acggtctgac tgtcctgccg
     2581 caaaccgaga agtttcttca cggcaccaaa gtcgcttacg gaattctggt gcaaagcgcc
     2641 ttgctgggtc aggatgatgt gctggcgcaa ttaaccgggg cgtatcagcg ttttcatctg
     2701 ccgactacgc tggcggagct ggaggtggat atcaataatc aggcggagat cgacaaagtg
     2761 attgcccaca ccttgcgccc ggtggagtcc attcattacc tgccagtcac gctgacacca
     2821 gatacgttgc gtgcagcgtt cgaaaaagtg gaatcgttta aagcctgacg tgcaaattta
     2881 gcagcagcgc gcgccgcctt ttccaccgta gcgcgcgtcc tgccgctcac ggaaaaactc
     2941 ctcgtaggtc atcggcgttt gatcgggatg attaacccgc atatgttcga catagttgtc
     3001 gtaatcaggc ataccaatca tcagcttcgc cgcctgacct aaatattttc cggcttttgc
     3061 cagtgaatca aacatcttgg cctccgctaa ccatgttgct gaaagagtgc cggatgcggc
     3121 gtgaacgctt tatccggcct acaaatgcta tcaacgcact cggtcagtcc cctcgcccca
     3181 tcggggagag ggttagggtg agggggaacc ctcggcacta tgccaacatt cgccaacatc
     3241 ccccctcact ctgactttag tgcgcgcctt tcgcctgcgc tacgatctcc tcgacatttt
     3301 ccggcatcgg ctcatacggc gtttctttcg ccgttggctt cggatctttc aatgccgcaa
     3361 gtgccgtctt aatcgagaac agtgccagga ccaccacgac caccataaag aagatggtta
     3421 acccggcatc cagacggttg ttgaacacca gttgcgccag ctgtgactca gtatactgcg
     3481 acggaatatt gccgctgtcg atcattgcct ggaacttgtt agcaatgacc aggaagccga
     3541 ctttcgcatc cgggctaaac gctttcagcc agtcagcggt cagggtacaa atcagcagcc
     3601 aggccgttgg taccagcgcc acccaggcgt aacgttgacg tttcatcttg aacaacacca
     3661 cggcacagag catcagcgcc atccctgcca gcatctggtt ggcaatacca aacagcggcc
     3721 acagagtgtt aataccgccc agcggatcga ccacgctctg atggaggaag tagccccacg
     3781 ccagtacgca cagcgctgtt gccagcaggt tagcaggcag tgaatcggtc cgcttcaggc
     3841 caggagacac cacgcccagc agatcctgca acataaagcg cgcagcacgc gtacccgcat
     3901 ctaccgccgt cagaataaac agagcttcaa acagaatggc gaagtgatac cagaacgcca
     3961 catccatcat accacccagc gcaccgtgca ggatgtaagc catccccacc gccaacgtcg
     4021 gcgcaccgcc cgcacgggaa atgatcgact gttcacccac ttcgctggca atctggttga
     4081 gcgtatctgg tgtaatgcta aagccccagc tactcaccac ctgcgcggca gaagcgacta
     4141 catccgccgt ccctgccgga gccagcaccg ccattgggct gttcatggca aaatacacgc
     4201 ccggatcgat gatacaggca gaaactagcg ccataatcgc cacgaaggat tccatcaaca
     4261 tcccaccgta gccgataaag cacgcctgcc cttcgttcgc cagcatcttc ggcgtggtgc
     4321 cggaagagat cagcgcatgg aagccagaca ccgcaccaca ggcgatggtg ataaacagga
     4381 acgggaacag gttaccggtc cataccgggc cagtgccatc gacaaatttg gtcagcgcag
     4441 gcatggtcag cgtcgggcgc ataatcaaaa tgcctaccgc cagaccgacg atagtcccga
     4501 ttttcaggaa ggtcgagaga tagtcacgcg gggccagcag caaccacacc ggcagcaccg
     4561 ccgccacaaa accgtagccc accagcatcc aggtcagctg cacgccggta aagtcaaagt
     4621 acggtgccca ggtcggactt tctgccaccc agccgccaga gataatggcg aaaatcagga
     4681 acaccaggcc aatcactgac acttcgccaa tgcggcccgg acgcagatag cgcaggtaga
     4741 ttcccataaa aatcgccagc ggaatggtga acgcaacggt atacgttccc cacgggctat
     4801 gagtcagggc tttcaccacg atcatcgcca gtactgcaag gataatgacc atgatcataa
     4861 agcaggctac cagcgcaatc accccggcag ttggccccat ctcttctttg accagctcac
     4921 ccagcgagcg accgtcacgg cgcgtagaaa caaacagcac catgaaatcc tgcaccgcac
     4981 cggcgagaac caccccggcg agcagccaga tcatccccgg caggtagccc atttgcgctg
     5041 ccagcaccgg ccccaccagc ggccctgctc cggcaatggc cgcaaaatgg tgaccgaaca
     5101 gcactttctt gtccgtcggc acatagtcca gcccgtcgtt atggcgcacc gctggcgtca
     5161 tacgcgtcgg gtcaaccgcc agcacatttt tggcgatata cagcccataa aaacggtaag
     5221 cgatcagata gatacagacc gacgccacca caatccacag cgcgttgatc tgttccccac
     5281 gatttaaagc aatgtatccc agagcaaatg ctcccattac agagagcact gtccagacga
     5341 ggtatttccc tgatttgttc atagttgtta tccgtgtgcg tgtccataga gatgttacat
     5401 tttgtttcta taacatcttt gtacgattta acaacacggt aaccatgtaa atcggagttg
     5461 aaaccaatat ttaacttaac aatgttaact cgatcactcc gttaaccgag ttttcctgca
     5521 atctcattta attctgtcgg gctcatccca aaactgccgt acccagccga caagtgcagc
     5581 aacgccgccc ctgttcatcg aaaacgacga tttcccaact ctgattttgc ctcccaagat
     5641 gcagcggctg gcagacgccg cgtaccttgc cttcagacac cgggcgatga tgggtggcgt
     5701 taagttccgt gcccaccaca cactgcccgt cacgggtcat cataaatccg gccatcgatc
     5761 ccagcgtttc agccagcgcc gccgacgcgc cgccatgcag caggccaaac ggctgatgag
     5821 tacgggtatc aaccggcatt tcggcttcca gcacatcatc tcccagaagg gtatacacaa
     5881 ttcccagatg cgccaccatt gtgttatcgc tggtggcgtt cagttcgtcg agcgttaaat
     5941 ggcgtttcca gatcatgctt atgcccccag cgttgagccg ccatcgacca caatatcctg
     6001 tagggtgata tggctggcga ggtcagaggc gaggaacaaa atcgtgttgg cgatctcttg
     6061 tggacgggcg attttcccca gcggaatgcc gagtttaaac tgctcgccaa agccgcgaat
     6121 acgctgttct tcggcgtcat cgctcaccca cagcgtgcgt tgcatatcgg tgtcggtgga
     6181 gccaggcgaa accacattac agcgcacgcc gctacccgcc agttccagcc cgacgctcaa
     6241 cgccaggctt ttcagcgccg ctttcgatgc gccataagca ctcataccaa tacgcgccgt
     6301 gtgtgcggcg tcggacgcca cagtgacaat cgccccgccc cgctgacggc gaaactggtt
     6361 catggtttgc tggaacaggt taaacgcacc gccgacgtta accgcaaaag tctgctgcca
     6421 gtcctcttta ctgagctgat cggtcgcgcc catgcgcaaa attcccgccg cattgatcag
     6481 cacgtccagt cgctccgttt cagctaacag tcgctgacat acctgcgcca cctgcccggc
     6541 gtcggcaaca tccattactt cggtcgcaaa gggatattgc tcctgagtga acgcttgatc
     6601 aaaacctgta actttcgctc ccgcctcaac aaacgtcagc gccgtggcgt agccgatacc
     6661 tttacctgcg ccggttaccc agacattttt accgctgaaa tccattattt cacctcgcgg
     6721 gagagtagct tccaccaggc gtcgatggtc gggtttttcg ccagcatgac aaagtcgata
     6781 tcaccatgca ctttgcgcca gcgcgccgcc agcgccatca tgcgcaccga atccagaccg
     6841 tagtcgatca ggttgtcgtc atcgaacggt tcatcggact cgtccagcaa cggcaggatc
     6901 acctcacgca gcgccgcttt gctggcgggg ataggtgctg gcagtaattc ttcggtcatc
     6961 accacccggc cagaacgtcc ggccacatat ttcagcgaca tcaaatgctc gtcacggctg
     7021 aaatcagcca gcgcatccgc caccataaac ggtttaatat cgcgcataaa tgcgtcggtt
     7081 gcggtggtca tacagccaat gtgggcatat actcctgtaa taatcagctg gttacgtcca
     7141 ctctctttga gcatttgctc cagcggagaa cgatgaaacg cgctgtagcg ccacttcacc
     7201 agcaccgtgt cgtcggcatc tggcgtcagg cgatccacca ccttttgctg ttccggcgag
     7261 cgggtcaggc ccggccccca catatcattc aacagcgccc gatcttcatc gctctgctct
     7321 ttcggctggg cggtgtaata aaccgggata ttgtgctgtt tgcagtagtc gcgcagcgca
     7381 gcaatattcg cgatcacctg ctccatcatc gggcagttct cgccccagaa gctgacaaaa
     7441 tagtcctgca tatcatggat taacaacgcg gcacgttgcg gttcaaaggc ccagtcaact
     7501 ttattctgcg gaatatcgtg agactccggc agtgcgtaag cctgtaattt tggaatagcc
     7561 atcgtgttct ctccttcagg ctgatgcgcg tgacgccagc cactgacgta attgtttttt
     7621 atcgactttc ccgaccgccg tcagcggaag tgaatccaca cactccacgc gatccggtaa
     7681 tttaaattcg gcaataccct gttcacgcag gaaacgacgc acctgcaccg cgcgcagcgg
     7741 ctcttttacc accagataag cacagctttt ttcgcccatc agctcatttt ccatgctcac
     7801 cagtgcggca tagatcactg ccggatggct cagcagcagg ttttcgatat cttcggcagc
     7861 gatcttctcg ccgccacggt tgatctgatc tttctcgcgc ccctgcacgg tgatgtaacc
     7921 ctctggatca atagagatca gatcgccgga acagtaaaaa ccgttggcat caaaggcact
     7981 ggcattgtgc tgcggacttt tgtaatagcc acggaaggtg tacggcccgc gcgtcatcag
     8041 gcgtccaact tccccttgcg gcagtggatt tccttcggca tcggcaaccc acacttcatc
     8101 gtccggacac attgggtaac cctgggtatg gataattttc tccgcgctat catcaagacg
     8161 ggtgtagttc accagccctt ccgccatgcc aaacacctgc tgcaactggc agccaatctc
     8221 agcgggaata cgcgctgcaa gcgtggcaga aagacgtgcg ccgccgacct gtaacagttt
     8281 cagcgaggca agctgcgccc ggctttcgcc ttcagccagc gcctgcaacc acaggctgac
     8341 tgccggcggc accagcgcgg taacgttaac ctgatgtttt gcaatcaacg ggaagcaaag
     8401 cgtggcgctg ggatcggccg ccagaacaac cgttcctccg gcaagaaaga cgcccagcga
     8461 tcctggcgaa ctcatagtgt agttatgagc cgccgggatc gagcacaggt agcgcgtctg
     8521 ttgtgtgaac tgacaaatct cggcgctacg acgcacgctg tagtaatagt cgttatgagt
     8581 gcgcgggatc agtttcggtg tgccggtggt gccgccggaa agctggaaat aggccacttc
     8641 atcagcaggt gatggcgtgg cagtaaaacc ctctgccgga tggttaatcg catcctgcaa
     8701 gttatgctca ccgctgtcgt tgtgcagttg caccacgcga atggaagaat gctctgcaac
     8761 aaatgtgttg aggaaatcat ccccgctaaa tagcgcatgc tggcgatcgg caatcagtaa
     8821 cgccggttca atctggctgg cgtaggcgtt cagttcacta cgctgatggc tgaacagcgc
     8881 cagcaccggc gcgacgccca gtttcagcag cgcgaaaaag gtaatgtaaa gttcagcgac
     8941 gttacccagt tgtaccagcg cggtttcacc aggtttaatg ccctgacggc gcaaactaca
     9001 ggcgaggtta tccgccgcct gattcagctc ccgataactc aactgccgct cgccgtcgat
     9061 aaccgcgatg ctgtcactcg cagcgtggcg agtcagaatg tcggtcagtg gcaaatcctg
     9121 ccagtagcct ttttcccgat agcgacgggc aaactcttcc ggccagcggg tgaatggaat
     9181 gctcatcctc gctccttaat gcaatccaaa aacgttcaac atggtagaaa gtttgacgcc
     9241 tgtttcgcgc cactcaccca acggtgacga cgcaggcaca atccccgctc cggcaaacag
     9301 acgcacctga ttttcccgca gcttcgcgca gcggatggtc accacccatt cgccgttacc
     9361 ttcgctgtca caccaaccca caatgccgcc aaacagttcg cggtcgaacg gctccagttc
     9421 agcaataacc tgggtcgcgg cctgatgcgg gaaaccgctc agcgcggggg tcggatgcag
     9481 cagacaggcc agagtcagtg cgttttcttg cgaattcgct ttaccttcaa agggagttgc
     9541 gagatgccac agcgtcggcg tggtaatcaa ttgtggagag gagggaacgt gtaactcact
     9601 actgcgttcg cgcagtacct ctttcatcgc ctgagtcccc agttcatgtt catggcgatc
     9661 tttttctgac gccagcagac gattacccgc ttcgcgatcg agcacttcat ccggctgacg
     9721 acgcgcggaa ccggctaacg gaatggagct aaaacgctcg ccgtctttac gtagcagcag
     9781 ttccgggctg gcccccagca ggacgccacc atcagccagc gggacatgga agttgtaact
     9841 aaccgggttt tgcgcaatca accgttccag caatacgcca ctatcaatgg cggcgtcagt
     9901 ggtgatatca atcaatcgtg acaacaccac tttgtcgacc tgcggcgtgg cggtaagtgc
     9961 ggcggcgcgg gcacccatct gttcaaacgt ggtttgttca ggaattgcct agcgttccac
    10021 cacgttcagc gactggctgc gggtaaaaca gcgggctgag gtctgttttt cctgacggga
    10081 gaacgactgc taggattcgg gaatatacag cgacgaaggc tgacgtggat cgaagggaat
    10141 cgccccgacc atcaccggat ttttgatgcc ctgcgctttg gcatcggcaa acagcgcggc
    10201 gagtttttgc tggaagggac tgtcgggcga atccccgttc acagccggtt catcgaaacg
    10261 ggcgaaacat cctgacgtcg taaaactgcg gtacggcgac ataaagaaaa agcgattggg
    10321 cgcaagtgtt gccatggtct gctgtacttc ctcagccagt gacgtatcca tatcatcctc
    10381 cacaaaatga taaaggcttt aataatgatt atcacttata ttttcggccg ctaacctaaa
    10441 agcaaacgcg cactatgtca actcttgagg taacgcaatc tgcaactcgg ggttgcatct
    10501 agttgtggtt acaatgaaaa tgagaagcat tattgatgaa ttcgcataag cgcaatgtga
    10561 tgtcctgcgc cgttttgccc cctctcccca aagggacgga ttgtgcgctt tgtcgaattt
    10621 gtcattacgc ccttaacctt attaataaca ggaagctgac ttgtgagact cgccccgctc
    10681 taccgcaacg cccttctatt aacaggactt ttgctttcag gaatagccgt agttcaggcc
    10741 gccgactggc cgcgtcagat tactgacagc cggggcactc ataccctgga aagccagccg
    10801 cagcgtattg tttccaccag cgtcaccctg accggatcac tgctggcgat tgatgctccg
    10861 gtgatcgcca gcggcgcgac cacgccgaat aaccgcgtcg cagatgacca gggcttttta
    10921 cgccagtgga gcaaggtggc gaaagaacgc aaactgcaac ggctctatat cggcgaaccg
    10981 agcgccgaag ccgttgccgc gcaaatgccg gatctgattt taattagcgc aaccggcggg
    11041 gattcggcgc tggcgctgta tgatcagctt tccaccatcg ccccgacatt aatcatcaat
    11101 tacgacgaca aaagctggca gtcgctgtta acgcagcttg gcgaaattac cgggcatgag
    11161 aaacaagcgg cagagcggat tgcacagttt gataagcaac tggcagcggc gaaagagcaa
    11221 atcaaattac cgccgcagcc ggtcactgcc attgtctata ccgccgctgc acacagtgcc
    11281 aatctctgga cgccggaatc agcacaaggg cagatgctgg aacaactcgg ctttacgctg
    11341 gcgaaattgc ccgcagggtt aaacgccagc caaagccagg gcaaacgcca tgacatcatt
    11401 cagcttggtg gggaaaatct ggctgcgggg ttaaacggtg aatcgctatt cctgttcgct
    11461 ggtgatcaga aagatgccga tgcgatttat gccaatccac tgctcgcgca cctgcctgcg
    11521 gtacaaaaca agcaggttta tgcgctggga accgagacgt tccgcctgga ttactacagc
    11581 gccatgcaag tgctggaacg gcttaaggcg ctgttttaag cattaactgt cggacgctgt
    11641 cacctgcggc ggcgtctggc gaaaacgtcg caactccacc agcaccagca ataacaacac
    11701 gccgataatc aacaaaccaa aaccgctcgc gcttgcggaa gcaaccggtg tcatcatcgc
    11761 gcccaaacca cccagcagcg ccgcgcctat ggcatcgccc gtcacgttct gcgccgtcca
    11821 caaaccgtta atccgcccta acatcgcttc cggggtttgc gtttgcagca ttgtatattg
    11881 cagcaacgaa ctaaccgcgc tcagccagcc gaacagcgcc agacaaacca cgcctaaaat
    11941 ccacatcggc atcaggccaa acagaccaat ggcgaggaac gatcccagcg tggagagcag
    12001 catcaataac cctggtcgcg cactatgtgc cagcttcccg ctggttaacg caccaatagc
    12061 cgcgccgagc gggatcgccg cgtagagaaa accaatctgt gccgctgaca tctgccagtt
    12121 gtcagccagc gccggataca gtaccagcac cgcgctcgcc atcgtcaata aaccacccag
    12181 cagcgggctg gcaagcagaa aacgaaatcc tgccagtaat gatttcaacg gatgctcaag
    12241 cggctgcggt ggcggtggca actccggaag gcttaataac ggtaacaagg taataaacgt
    12301 gcccgccgcc gccagcccgt agttccaggc tacgccgccg gtcgccagca ataaaccgcc
    12361 aatcatgggc gaaatcaccg accccaggcg cacggtcaac atggtgatcg ccccggcctg
    12421 cattaagttt tcacgcccta ccagtgcaga tgttgccgcc agtagcgccg taacaccaag
    12481 tgatgcgaaa aaaccatccc ataaaccaag taaatagatt gccagcaatg acggctccgg
    12541 cagcagtgca ttaaggcaca gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa
    12601 aatcactttt ttgcgttcat agcgatccgc cagcacaccg ccaaccatca ggccaacaaa
    12661 catcgcgccg ccggtcagcg tcaccgaaag ccccacctgc caggtagaat gggtcatcat
    12721 ctggatctgc accggcaccg cgacgccgag caaacccaga gacacaattg agataaaacg
    12781 agcgaggaat actgcgcgaa acgccgggtg cgttttcaac aggctgaggt taagcagcca
    12841 ggattgttta ttcattacaa tgccttgcca tcaaatattt taaataccta ttcttaggct
    12901 gcacatgtta acatatccaa ataagatcga taacgataat taatttcatt atcatggaag
    12961 ttcgtatatc tggttctgtt gccgtgacac gcgccattgc cgtgcccgga ttgctgttat
    13021 tactgattat cgcgacggca ttaagcctgc tcattggggc aaaatcactc cccgcttccg
    13081 tagtgctgga ggccttctcc ggcacctgcc agagcgccga ctgcaccatc gtgctcgacg
    13141 cgcggctgcc gcgtaccctt gccggtttac tggcaggcgg cgcgcttggc cttgccgggg
    13201 cgttaatgca aaccctcacc cgaaacccac ttgccgaccc cggcttgctt ggcgtgaacg
    13261 ccggagccag ctttgccatt gtgctgggtg cggcgctgtt tggttactca tccgcacagg
    13321 aacaactggc gatggccttc gccggggcgc tggtggcctc attgattgtt gcctttaccg
    13381 gcagtcaggg tggagggcag ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg
    13441 cggcggtgct ggaaggactg accagcggca tcgccctgct taatcctgac gtctacgatc
    13501 aactgcgttt ctggcaagcc ggttcgctgg atattcgcaa tctacatacc ttaaaagtgg
    13561 tgctgatccc ggttctgatc gccggagcaa ctgcgctatt actgagtcgc gcgctgaaca
    13621 gtttgagcct cggcagtgac accgcgacgg cgctgggcag tcgcgtggcg cgcacacagt
    13681 tgattggtct gctggcgatt accgtgcttt gtggtagtgc gacggcggta gttggcccga
    13741 ttgcctttat cggcctgatg atgccgcaca tggcgcgctg gctggtaggt gccgatcatc
    13801 gctggtcgct gcccgtcacg ctacttgcta cccctgccct gctgctgttt gccgatatca
    13861 tcgggcgggt gattgtgccc ggcgaactgc gcgtttctgt ggtcagtgcg tttattggtg
    13921 caccggtgct gatcttcctc gtgcgacgta aaacgcgagg tggtgcatga tttacgtctc
    13981 tcgccgatta ctcatcacct gtttgctgct gatttccgcc tgtgtggttg caggtatctg
    14041 gggattacgc agcggtgccg tcacgctgga aatctcgcag gtattcgccg cgttgatggg
    14101 cgatgcgccg cgcagtatga cgatggtggt caccgaatgg cgtttaccac gcgtgctgat
    14161 ggcgctgttg attggcgcgg cgctgggcgt cagcggcgcg atttttcagt cgctgatgcg
    14221 taacccgctc ggcagccctg acgtaatggg ctttaacacc ggggcgtgga gcggcgtgct
    14281 ggtggcgatg gtgctgtttg gtcaggatct gacggctatc gcgctggcag caatggtggg
    14341 cggcattatc acttcgctgt tggtctggct gctcgcctgg cgtaacggta tcgacacctt
    14401 tcggttgatt attatcggta tcggcgttcg cgccatgctg gtggccttta atacctggct
    14461 gttgctgaaa gcgtctttag aaacggcgct aacagcaggt ttgtggaatg ccggatcgct
    14521 caacggcctg acgtgggcaa aaacctcgcc ttccgcaccc atcattatat tgatgctcat
    14581 tgccgccacc ttgctggtgc ggcgaatgcg cttgctggag atgggcgatg ataccgcctg
    14641 cgcgctgggc gtcagcgtcg agcgttcgcg tctgttaatg atgctggttg cagtggtgct
    14701 taccgctgcg gcaacggcgc ttgccgggcc gatttccttt attgctttag tcgcgccgca
    14761 cattgcccga cgcattagcg gcaccgctcg ctgggggctg acccaggcgg cgctgtgcgg
    14821 agcgctgtta ctgctggcgg ccgttctctg cgcccaacaa ctgtttatgc cgtatcaact
    14881 tccggttggc gtcgttaccg tcagcctcgg cggtatttac cttatcgtct tgttaattca
    14941 ggagtctcgc aaaaaatgac cgaatcagta gcccgtttgc gcggcgaaca gttaaccctg
    15001 ggatatggca aatataccgt tgcggaaaat ctgactgtag aaattcctga tggtcacttc
    15061 acggcaatta tcggaccaaa tggctgtggt aaatccacgt tactgcgtac cttaagccgc
    15121 ctgatgacgc ctgctcacgg gcatgtctgg ctggatggcg agcacattca acattacgcc
    15181 agtaaagaag ttgcacgccg gattggtctg ttggcgcaaa atgctaccac gccgggcgat
    15241 atcaccgtgc aggagctggt ggcgcgtgga cgttatccgc atcaaccgct gtttacccgc
    15301 tggcgcaaag aggatgaaga agcggtaacg aaagcgatgc aggccacggg aataactcat
    15361 ctggcagatc aaagcgtgga taccctttct ggcggacaac gccagcgagc gtggatcgcg
    15421 atggtgctgg cccaggaaac ggcaattatg ctgctcgacg aacccacgac ctggctggat
    15481 atcagtcatc agattgatct actggaactg ttaagcgaac tgaaccgcga gaaaggctat
    15541 accctggcgg cggtgctgca cgatcttaat caggcctgtc gttacgccag ccatttgatt
    15601 gcattgcggg aagggaaaat tgttgctcag ggagcgccga aggagattgt cactgctgaa
    15661 ctgattgagc gcatttatgg tctgcgatgc atgatcattg acgatccggt agccggaatg
    15721 ccgcttgtgg tgccgctcgg acgaacggca ccttcaaccg caaatagtta aactaagtgg
    15781 tctgccatca tggcatcctg ttttctggat gccatcgcat agcgcaataa cacgctacca
    15841 caagccacca tcccgccgat cagcgcggaa aggatcacaa taatcgcctt acccggaccg
    15901 tcttttttca ctggcaatga cgggcttaac tgatatttaa acggcgtaaa attcacatcg
    15961 ttgacatttg cttttgttaa ttgctcgaca agatactgcc gattacgtaa ttcaccgttc
    16021 agttccgcaa cgtcagtaac cgctttttca atttccagtt tgcgttcaat accgtctgcg
    16081 ccgagggaaa tagagaaatc ggggtcatct ttaacggcct gaccattact gtatacgggc
    16141 tttttaattc ctgccgcgtt ggcaatgtcg agtgaataat tgaggcgctg aatgtttgca
    16201 tcaagttgat ttttcgtttt aatgcgatcc tgagccagtt tttctttttc aaactgggtt
    16261 ttgatctcca gtttattacg gacgttttct atcgactctt tcaccaccaa cgcagagata
    16321 taatcgatat acccgctcaa aacggtctgc gcctctttac tggttggcgc ggtaaaactt
    16381 agcgtccagg atgtatacag tgacggttca tcttttttct tactagcatt gtcatcaacc
    16441 gctttcattt tttcgctcaa tgcaacaata gcgcgatgca aatccagttc atcgattttc
    16501 gcctctttta attagtccat cacataaggt gatgaacgca ggtactcttc cagcaagcta
    16561 acagactgaa acttcttgat aaacaggtta aatgcaccgc cacggtcgat attaacatcg
    16621 agatcaagaa cgcggagttt ggtaaaggtt ttctccaggt cctgccactg aatggcttcg
    16681 gcgggagtaa taacggctga gctggtccat ttttgcggca ggatgaaaga gatcagtaaa
    16741 cctgcgcagg caaatgcaaa aacgaccgcc atgaccgttt ttttggcccg ccataaaact
    16801 tcgattagat taagtaaatc aattt
//
LOCUS       DAJTQQ010000083        16204 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000083 DAJTQQ010000000
VERSION     DAJTQQ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16204
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.083"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..106)
                     /locus_tag="OO309_002985"
     CDS             complement(<1..106)
                     /locus_tag="OO309_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HCR5848049.1"
                     /translation="MSKEFYARLAAIQENLNAPKNQYNSFGKYKYRSCE"
     gene            complement(363..515)
                     /gene="kil"
                     /locus_tag="OO309_002986"
     CDS             complement(363..515)
                     /gene="kil"
                     /locus_tag="OO309_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HCR5848050.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINI
                     PWYRRK"
     gene            complement(500..634)
                     /locus_tag="OO309_002987"
     CDS             complement(500..634)
                     /locus_tag="OO309_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848051.1"
                     /translation="MMHFQLAGSGVMSAFYPHESELSRRVKQLISAAKKQPEALCAMK
                     "
     gene            complement(829..1299)
                     /locus_tag="OO309_002988"
     CDS             complement(829..1299)
                     /locus_tag="OO309_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848052.1"
                     /translation="MTKSWSVPFPESETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWLQEWKQKRNRYAIKKVAKNAERSFAFPTKKLAI
                     ESLLRRKKYHLMRIKQDLAVVSTLVDGMKNIDTSTPDIEYNFGHNQETENWVFY"
     gene            complement(1308..1634)
                     /locus_tag="OO309_002989"
     CDS             complement(1308..1634)
                     /locus_tag="OO309_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HCR5848053.1"
                     /translation="MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKP
                     KSRVESALNPIDLTVLAEYHEQIESNLQRIERKNQRTWYSKPRSEMGVTCVGRQKMKL
                     GSKPLI"
     gene            complement(1998..2402)
                     /locus_tag="OO309_002990"
                     /pseudo
     CDS             complement(1998..2402)
                     /locus_tag="OO309_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618033.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2399..3031)
                     /locus_tag="OO309_002991"
     CDS             complement(2399..3031)
                     /locus_tag="OO309_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCR5848054.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGDGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPMTPINGNCKIIGVVVEARVKFV"
     gene            3135..3350
                     /locus_tag="OO309_002992"
     CDS             3135..3350
                     /locus_tag="OO309_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5848055.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            3467..3748
                     /locus_tag="OO309_002993"
     CDS             3467..3748
                     /locus_tag="OO309_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015995142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848056.1"
                     /translation="MGTIATKSKKAARIESALLNKLALMGQKTFARAMGVPEYQVSRW
                     KNGFFSQVSMMLAVLEYGIEDDEMAELTKRLADYLTKEKAPKNGEFFEA"
     gene            3783..3944
                     /locus_tag="OO309_002994"
     CDS             3783..3944
                     /locus_tag="OO309_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848057.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            3931..4761
                     /locus_tag="OO309_002995"
     CDS             3931..4761
                     /locus_tag="OO309_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCR5848058.1"
                     /translation="MGVVKLADYRPQLEVVEHRVAELEDGYTRTANTLLEAAMLSGLT
                     LHQLLIVMAVWRKTYGYNKKIDWIGNEQFAELTGMAPTKCSTAKNELIRMGVLIQVGR
                     QVGMNTNISEWKTKVNGFGKTFTNSVKQTFTKSVKSNLPNQSNTKDNIQKTINTNTPL
                     PPNGGGDGQVKPERRKAERIDYESFLNAYNTEVGDRLPHAVAVNEKRKRRLKKIIPQL
                     KTPNVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLLREDSLTGVREGKFADRGIA
                     "
     gene            4758..6134
                     /locus_tag="OO309_002996"
     CDS             4758..6134
                     /locus_tag="OO309_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5848059.1"
                     /translation="MKQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFE
                     VIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLV
                     LEIMDEMREPIQSGTIDASSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
                     RLRNGEESDTLKTGIDELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIP
                     GSDVRRGVLIFSMEMSALQIAERSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERHKQENPNLSLIMADYLGLIEKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDENSSAAPFAEIIVTKNRFGSLGTVYQRFCNGHFVACDQDEARQICTASNAPAARGR
                     RYAQGADV"
     gene            6207..6533
                     /locus_tag="OO309_002997"
     CDS             6207..6533
                     /locus_tag="OO309_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848060.1"
                     /translation="MADWQIPIIILAGASLVAGFILLKKHKDRDQKVEVLYGYPANST
                     TWLTIYHYRKSGRWVFEWDDLFAEKRPKSWGDISECMMFEERKSGATREEFNEAWARL
                     SERGYL"
     gene            6530..6730
                     /locus_tag="OO309_002998"
     CDS             6530..6730
                     /locus_tag="OO309_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848061.1"
                     /translation="MSKYEKLDQNILSMLSERPTPVFDIWLKWRSNGMYIETIDRRMQ
                     YLRKKGLVANVRGKGWVKINLS"
     gene            6742..6930
                     /locus_tag="OO309_002999"
     CDS             6742..6930
                     /locus_tag="OO309_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004904153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848062.1"
                     /translation="MDDSLEIFEEWCADELGVSAYFIRQMRSKNILGVIEYKRVEINK
                     RYRAWMAAVRAAGVKVKE"
     gene            6933..7097
                     /locus_tag="OO309_003000"
     CDS             6933..7097
                     /locus_tag="OO309_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848063.1"
                     /translation="MSAYEEIMLALRFFFGVEEDENVNEIIGQDHDPIGTIAAALDDY
                     RSVNGEETNV"
     gene            7078..7518
                     /locus_tag="OO309_003001"
     CDS             7078..7518
                     /locus_tag="OO309_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HCR5848064.1"
                     /translation="MRKLTFELRSPIHQQNAIQAIQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGRSTS
                     RMRVGEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDKTE"
     gene            7515..8105
                     /locus_tag="OO309_003002"
     CDS             7515..8105
                     /locus_tag="OO309_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine-binding protein"
                     /protein_id="HCR5848065.1"
                     /translation="MNKYRLIYADPPWQYRDKANDGNRGAGHKYDVMNVQDICRLPVW
                     DLADPESCLLAMWWVPTQPAEALKVIEAWGFRLMTMKGFTWHKTNKHKGNSAIGMGHM
                     TRANSEDCLFAVRGRLPERMDASICQHFTAPRMEHSAKPPIVRDMLAKLLGDVPRCEL
                     FSRDKVTGWDMWGNQCDSDFELAPGMAIKPCKMVIA"
     gene            8102..8275
                     /locus_tag="OO309_003003"
     CDS             8102..8275
                     /locus_tag="OO309_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninD"
                     /protein_id="HCR5848066.1"
                     /translation="MKHCYRCGERKEDDRFRPGQPYWNRWCLRCERTPTGVLPLPQEK
                     EDVWRDSDEVSPT"
     gene            8242..8417
                     /locus_tag="OO309_003004"
                     /pseudo
     CDS             8242..8417
                     /locus_tag="OO309_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
     gene            8420..8821
                     /locus_tag="OO309_003005"
     CDS             8420..8821
                     /locus_tag="OO309_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848067.1"
                     /translation="MLLIQPGFGLSIKKGHMFGEKESQRKMMSIRLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVENHHAVRQAKRKANMTYMKAYRKAWKEHRDRYQQDMEKL
                     ESENMELRRKLGEAKRDIDAYKRLFNGESHA"
     gene            8781..9011
                     /locus_tag="OO309_003006"
     CDS             8781..9011
                     /locus_tag="OO309_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninF"
                     /protein_id="HCR5848068.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCEHCCAELMSDPNSSMYEEEDDGQTSAKTM"
     gene            8983..9594
                     /locus_tag="OO309_003007"
     CDS             8983..9594
                     /locus_tag="OO309_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HCR5848069.1"
                     /translation="MAKPARRRCKNEECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCI
                     SCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIG
                     QEAVDEIESNHNRHRWTVVECKAIKAEYQQKLKDLRNSRSEAA"
     gene            9591..9797
                     /locus_tag="OO309_003008"
     CDS             9591..9797
                     /locus_tag="OO309_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HCR5848070.1"
                     /translation="MTFSVKTIPDMLVEAYGNQTEVARRLKCSRGTVRKYVDDKDGKM
                     HAIVNDVLMVHRGWSERDALLRKN"
     gene            9775..10440
                     /locus_tag="OO309_003009"
     CDS             9775..10440
                     /locus_tag="OO309_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCR5848071.1"
                     /translation="MRYYEKIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NIDYDKEILAKALSHKADELPLIIELVSKDKKYVICHADYPCDEYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            10437..11060
                     /locus_tag="OO309_003010"
     CDS             10437..11060
                     /locus_tag="OO309_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5848072.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            11493..11816
                     /locus_tag="OO309_003011"
     CDS             11493..11816
                     /locus_tag="OO309_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HCR5848073.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            11800..12276
                     /locus_tag="OO309_003012"
     CDS             11800..12276
                     /locus_tag="OO309_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCR5848074.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            12273..12710
                     /locus_tag="OO309_003013"
     CDS             12273..12710
                     /locus_tag="OO309_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR5848075.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAIAYKEQRDKAASTI
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPRLTADAELNYYRLRSGIDRITAQVNYLQEYIRTQCLK"
     gene            12915..13457
                     /locus_tag="OO309_003014"
     CDS             12915..13457
                     /locus_tag="OO309_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5848076.1"
                     /translation="MNYPTVVNDIDFRDLIFVANNDPVTDSFMVAKAFGKLPKNVVRD
                     IERTIEACPPEFDTKLNFELCYKNNELQNGKPQKFYRLRKDGLMLLVMSYTKKEAMRI
                     KIAYINAFNWMYAMLQVGHRQFEEERNAVMLEYMKEKDVASMSGRLLNRWGKIKKPQL
                     MARIERLEQHGQTVIPGLIN"
     gene            13677..13892
                     /locus_tag="OO309_003015"
     CDS             13677..13892
                     /locus_tag="OO309_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR5848077.1"
                     /translation="MDDNGNQWVNVEHRLPESKEGMWSKEVIALTDTGDVFKLSCMGS
                     YWQRTKAFIDSGASKVTHWMPLNYPDD"
     gene            14040..14282
                     /locus_tag="OO309_003016"
     CDS             14040..14282
                     /locus_tag="OO309_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HCR5848078.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITIRTFEAVRKSKEALDLFTTGA"
     gene            14318..14806
                     /locus_tag="OO309_003017"
     CDS             14318..14806
                     /locus_tag="OO309_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCR5848079.1"
                     /translation="MAAPKGNRFWEARSSHGRNPKFESPEALWAACCEYFEWADDNPL
                     WEGKVFSYQGEIIKANVPKMRAMTISGLCTFLDITRQTWGTFRSMEGFSDVTSRAEDI
                     IYDQKFSGAAADLLNANIIARDLGLKEQSQVEDVTPDKGDRDKRRSRIKELFNRGTGR
                     DS"
     gene            14784..>16204
                     /locus_tag="OO309_003018"
     CDS             14784..>16204
                     /locus_tag="OO309_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5848080.1"
                     /translation="MELDAILDNLSDEEQIELLELLEEEENYRNTHLLYEFTPYSKQR
                     EFIDAGHDYPERCFMAGNQLGKSFTGAAEVAFHLTGRYPGTKGYPADGKYGGEWKGKR
                     FYEPVVFWIGGETNETVTKTTQRILCGRIEENGEPGYGSIPKEDIISWKKSPFFPNLV
                     DHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIHGVWFDEEPPYSIYGEGLTRT
                     NKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMTIYDAEHYTDEQKEQIIASYP
                     EHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHFYVIGGMDFGWDHPQAQVQLW
                     WDKDADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTAWPHDGNQHEKGGGEQLKGQY
                     ADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFKVFNTCEPFFEEFRLYHRDEN
                     GKIVKLNDDVLSAVRYAYMMRRFA"
BASE COUNT         4649 a         3651 c         4028 g         3876 t
ORIGIN      
        1 cttcgcagct tctgtattta tatttaccga atgagttgta ctggttcttt ggcgcgttca
       61 agttctcctg aatagctgcc agtcttgcgt aaaattcttt gctcatatgt ttgttctcag
      121 aatgggcatg gcccaaggaa ataacgctga tttaatactt cgactcggga caaattaagg
      181 catacccgca ttccttcgcg gtcgccatta tggcgatacc agagagcttt ctgcgtgtac
      241 atgcgtctct gtaacttgct ctccttcact gtggttgcaa gtgacatgaa tatctccttc
      301 gttaccgatt aattctttca tctgacgaat gaattcttcg tctgaccagt tatctgtaaa
      361 actcatttcc tgcgatacca cggaatattg atagctgatt tcatcgcttt atttgcttca
      421 agccacattt ttgaatcacc aataaatctg gctattactg ctttgttttg tgctgcacga
      481 agcatctggt gattgatggc tatttcattg cgcataacgc ctccggttgt ttctttgctg
      541 cactgattaa ttgtttaact cggcgtgata attcagattc gtgcgggtag aaagcggaca
      601 tgacgccgct acccgcgagc tgaaagtgca tcatggataa ctccttctgt ttgattgcat
      661 aacgaaaacg cctcgagtga agcgttattg gtatgcatat aaaaaagccc tcatactgga
      721 gggcaaagaa gatttcaaat aatcagaaca agtcggctcc tgtttagtta cgagcgacat
      781 tgctccgtgt attcactcgt tggaatgaat acacagtgct tactcgtact aataaaatac
      841 ccaattttct gtttcttggt tgtgcccaaa gttatattca atatctggtg ttgatgtatc
      901 aatattcttc atcccatcaa caagagttga tacaacagcc aaatcctgtt tgattctcat
      961 taaatggtat ttcttccggc gcaataaact ttcaatggca agtttcttcg ttgggaatgc
     1021 aaaagatctt tctgcatttt ttgctacttt cttaattgca tatctatttc tcttttgttt
     1081 ccattcctgt aaccactgat ttggtgctgg tttaaaatta acaatccaat gcgcaggaac
     1141 caaccatgca taatgctctg tctgatgaaa agctatatat tgaagtgcga atattttgat
     1201 tccatcttct tcaactgtcg cctggaatct ccagaaaaca ggcattccat catgttcagt
     1261 ttctgattca ggaaaaggta cgctccatga ttttgtcata tctcacctca aataagtggc
     1321 ttgctgccta atttcatttt ctggcgacca acacaagtca cacccatttc actgcgtggc
     1381 ttgctgtacc atgtgcgctg attcttgcgc tcaatacgtt gcaggttgct ttcaatctgt
     1441 tcgtggtatt cagccagcac cgtaaggtct atcggattca gtgcgctttc tactcgtgat
     1501 ttcggtttgc gattcagcga gagaataggg cggttaactg gttttgcgct taccccaacc
     1561 aacaggggat ttgctgcttt ccattgagcc tgtttctctg cgcgacgttc gcggcggcgt
     1621 gtttgtgcat ccatcttgat tctcctgtca gttagctttg gtggtgtggt aggtgggaga
     1681 cccatttcga cctgcttcgg ccgacttcaa ttcggcaata gttccgcagg cctcgccgct
     1741 ttacgtgcga catattccca tccatgaacc cttcaccaca ccccaaagcc ttctgctttg
     1801 aatgctgccc ttcttcaggg cttaattttt aagagcctca ccttcatggt ggtcagtgcg
     1861 tcctgctgat ggcttaaaat tacaagaaag attgtatgtt gtaaacaaga aatattgtaa
     1921 aaaggggcgt aaaaacaaac tccattgttt ttaaatgaaa aatagtttgt ttttggttat
     1981 cgagattgag gtggggatta ctggttgcag gttccgacta catcaccaac aaatgatttg
     2041 gttgatgtaa gttgttgcat acctgggata ttcattactt tggagtaaag agcttttttg
     2101 tctgtagtga ttgatcaagt ttcaacggtt attcctcctc cagactggta ttctcctacc
     2161 atagtgttcg atgacaaagc agtgtatttc atctctggat agacgccaga aactgattca
     2221 taaactgatg atttatcgcc atttattgtt acgtggaaaa cggaatcttc cgtgctgtct
     2281 tttgtaaact cgtaacgatc gccattcatt gacccgtacc cgtgcaggtt tgtgacaatc
     2341 cagcattcag aattggcgct ggtagttaag agtattgaga gtagcgccgc aatcctgatc
     2401 atacgaattt taccctcgct tccacgacaa caccgataat cttgcagttc ccgttgatag
     2461 gagtcatagg ccatgaaggg ttcaggcctt tcaggtactt ctgcccgcca tctataacca
     2521 gtttcttgaa tgttgcttcg ttcgcgtcag tcagtttggc tacaacaagg cttccattca
     2581 ctggctcgcg tccagtatct actaacacca tatgaccttc agggatgctt tgacctacag
     2641 gtgaggtcat ggaatcacct tcaaccttca gccagaatcc atcgcctaat aagttaacgt
     2701 cactgtcata ccattcatca atgtccttga tatcgtaggg ttcacaagct tcacaccacg
     2761 aaccagctct aaccatgcta atcaatggat atttcccttt gggctcaaca tgcccaacaa
     2821 atctaacatt cgaatcagag gtgccattga gcagccagtc aacacttacg ccaagagctg
     2881 acgcaagttc tggtaaaaag cgtggtcgct tagttttacc gttttcgagc tgctctatag
     2941 actgctgggt agtccccacc ttttgagcaa gttcagcctg gttaagtcca agctgaattc
     3001 ttttgctttt taccctggaa gaaatactca taagccacct ctgttattta cctccaatct
     3061 tcacaagaaa aactgtattt gacaaacaag atacattgta tgaaaataca agaaagtttg
     3121 ttgatggagg cgatatgcaa actctttctg aacgcctcaa gaagaggcga attgcgttaa
     3181 aaatgacgca aaccgaactg gcaaccaaag ccggtgttaa acagcaatca attcaactga
     3241 ttgaagctgg agtaaccaag cgaccgcgct tcttgtttga gattgctatg gcgcttaact
     3301 gtgatccggt ttggttacag tacggaacta aacgcggtaa agccgcttaa gacattcccg
     3361 ctcttacaca tcccagccct gaaaaagggc attaccagaa acaaatctct atggttttgc
     3421 gtttctttgc gaagccaact ctatctaatc attaaggaaa ttatctatgg gtactattgc
     3481 aactaaaagc aagaaagcgg ctcgcatcga gtcagccttg ctgaacaaac tggcactgat
     3541 ggggcagaag acattcgctc gagcaatggg ggttcctgaa tatcaggtaa gccgatggaa
     3601 gaatggtttc ttctcgcagg taagcatgat gcttgctgtt ctggaatacg gaatcgaaga
     3661 cgatgaaatg gctgaattga ctaagcggct tgccgattac ctgacaaaag aaaaagcccc
     3721 gaagaacggc gaattcttcg aggcctaatg tagaaagact ggatcaatcc acgggagtaa
     3781 ttatgacaaa acgtcgtaag aaataccagg aaaaagaaga gattcgacac cctgattcac
     3841 ctgagggatt agtggtagcc gcagcaaata acagggcgtt cgcagagcgc cttgttggtg
     3901 tttacagact agccaaagca ggagtgaaac atgggcgtcg ttaagttagc tgattacagg
     3961 cctcaactgg aggtcgtgga gcatcgcgtg gcagaactcg aagatggcta cactcggact
     4021 gcaaacacac tgttagaagc cgccatgctt tctggactta ctctacatca gttactgatt
     4081 gttatggctg tgtggcgcaa gacatacggt tacaacaaaa aaatagattg gatcggaaac
     4141 gaacagttcg ctgaactcac tggcatggca ccaaccaaat gctctaccgc caaaaacgag
     4201 cttatcagaa tgggagttct cattcaggtg gggcgtcaag ttggtatgaa tacaaacatt
     4261 tccgagtgga aaacgaaagt taacggattc ggtaaaacat ttaccaattc ggtaaaacaa
     4321 accttcacca aatcggtaaa aagcaattta ccgaatcagt caaacacaaa agacaatata
     4381 caaaagacaa taaatacaaa taccccctta ccccctaacg ggggcggcga tgggcaggtt
     4441 aaacctgaac gtcgcaaggc agaacgaatc gactacgaat ccttcctgaa cgcctacaac
     4501 accgaagtcg gtgacagact gccacacgct gttgcggtca acgagaaacg caaacgccgc
     4561 ctgaagaaaa tcatcccgca actgaaaacg ccaaacgtgg acggtttcag agcgtatgtc
     4621 agggcgtttg tgcatcaggc caagccgttt tacttcggag acaacgacac gggctggacg
     4681 gccgattttg attacctgct gagggaagac tcgttaacgg gggtacggga agggaagttt
     4741 gcagacaggg ggattgcatg aaacaggata tcgaagcgag cgttatcggt ggcctgctga
     4801 ttggtggatt aacaccaacc gccagcgacg ttctggcaac gctggagccg gaagcgtttt
     4861 caattccgct ctaccggaaa gccttcgagg ttatccgcaa gcaggcgcga aacagaaacc
     4921 taatcgacgc gctgatggtt gccgaggcgt gcggagagga gcatttcacg tcaatcctga
     4981 tgaccagcaa gaactgcccg agtgccgcaa acctgaaggg atatgctgga atggtcgcgg
     5041 ataactatca ccgccgtctg gtgctggaaa tcatggatga aatgcgtgaa ccaatccaaa
     5101 gcggaaccat cgacgcatcg agtcaggcga tggatgaact tgtaaagcgt ctttcagcca
     5161 tcagaaagcc ccgtgacgag gtaaaacctg tacggttagg ggaaatcatc actgactaca
     5221 ctgacacgct tgacaggcgt ctgaggaacg gagaagagtc agataccctg aagaccggaa
     5281 tcgacgaact tgacgccatc accggaggga tgaacgcaga agacctggtg attatcgccg
     5341 ctcgtcctgg tatgggtaaa accgaactgg cgctgaagat tgccgaaggc gttgcaagcc
     5401 gcgttattcc tggttctgac gtccggcgcg gagtgttgat tttctcgatg gaaatgagcg
     5461 cattgcagat tgcagagcgg agcattgcca acgccgggag gatgtcggtt agcgtgctgc
     5521 gaaatcctgc atcgatggat gacgaaggct gggcgcgtgt tgctaacggc atgagtcagc
     5581 ttgcagattt ggatgtatgg gtagtcgatg cctcgcggtt atcggtcgaa gaaatacgct
     5641 caatcgcaga acggcacaaa caggaaaatc caaacctgtc actcatcatg gcggattatc
     5701 ttggcctgat tgagaagccg aaagcagacc gcaacgacct cgcaattgct cacatctccg
     5761 gaagcctgaa ggcgatggcg aaagacctga aaacgccagt tatctccctg agtcagcttt
     5821 cgcgcgatgt tgagaagcga ccaaacaaac gcccgacaaa cgcagatttg cgtgattcag
     5881 gaagcattga acaggacgca gactcaatca tcatgctcta tcgggaagcg gtatatgacg
     5941 agaacagtag cgccgcgcca tttgctgaaa tcatcgtgac gaaaaaccgt tttggctcgc
     6001 ttggtacggt ttaccagcgg ttctgtaacg gacactttgt tgcatgtgac caggatgaag
     6061 ccagacagat ttgcacagca tcaaatgcac ctgctgcgcg tggcagacga tatgcacaag
     6121 gggctgacgt atgaccatct acatcactga gataataaca ggcctgctgg taatcgcagg
     6181 cctttttatt tgggggagag ggaagtgtgg ctgactggca aattccaatc atcattcttg
     6241 ccggagcttc gctggttgct ggctttatcc tgctgaaaaa gcataaagac cgtgatcaaa
     6301 aagttgaagt tctctatggg tatccagcga acagcacaac atggctgacc atttaccact
     6361 accgaaaatc aggacgctgg gtattcgaat gggatgatct gttcgctgaa aagcgaccaa
     6421 agtcatgggg agacatcagc gaatgcatga tgtttgaaga aagaaaatcc ggcgcaaccc
     6481 gagaagagtt taacgaagcg tgggcgcgat taagtgagag agggtatttg tgagcaagta
     6541 cgaaaaatta gatcaaaaca ttctttcaat gctgagtgaa agaccaacac ctgtttttga
     6601 tatctggctt aaatggcgga gcaatggaat gtatatcgaa accatcgatc gccgtatgca
     6661 atacctgaga aagaaagggc ttgttgcaaa tgtgcgtggg aagggttggg tgaaaattaa
     6721 cctgtcataa cggggattga tatggacgat agcttagaaa tatttgaaga gtggtgcgct
     6781 gatgaacttg gtgtctccgc ttacttcatt cgacaaatgc gaagcaagaa tattttgggc
     6841 gtgattgagt ataaacgagt tgaaataaac aagagataca gggcttggat ggccgcagtt
     6901 cgcgccgccg gagtcaaagt gaaggagtga gtatgagcgc atacgaagaa atcatgttag
     6961 ccctgcgatt ctttttcggt gtggaagaag atgaaaacgt aaatgagatt atcggacaag
     7021 accatgaccc gatagggact attgcagctg cacttgacga ttacaggagc gtaaatggtg
     7081 aggaaactaa cgtttgaact aagaagcccc atccatcagc agaacgccat tcaagctatc
     7141 cagcaaatcc ttccagaccc aaccaaacca atcgtagtaa ccattcagga acgcaaccgc
     7201 agcttagacc agaatcgaaa gctttgggct tgccttggtg acgtttcgcg tcaggttgaa
     7261 tggcatggtc gctggctgga tgcagaaagc tggaagtgtg tgtttaccgc agcattaaag
     7321 cagcaggacg ttgttcctaa ccttgccggg aatggatttg tggtaatagg ccggtcaacc
     7381 agcaggatgc gtgtaggcga atttgcggag ctattagagc ttatacaggc attcggtaca
     7441 gaacgtggcg ttaaatggtc agacgaagcg cgactggctc tggagtggaa agcgagatgg
     7501 ggagataaaa ctgaatgaac aaataccgac ttatttacgc agatccgcct tggcaatatc
     7561 gcgacaaagc caacgatggc aatcgcggtg ctggacataa atacgatgtt atgaatgttc
     7621 aggacatttg ccgactgcca gtatgggatt tagcggatcc agaatcttgc ttgttagcga
     7681 tgtggtgggt gccgacacag ccagccgaag cgctaaaggt aattgaggcg tggggattca
     7741 ggttgatgac tatgaaaggc tttacttggc acaaaaccaa taagcacaaa ggcaacagtg
     7801 cgatcggaat ggggcatatg accagggcaa atagcgagga ttgcttgttt gccgttcgag
     7861 ggcggttacc tgagagaatg gacgcttcaa tatgccagca ctttaccgca ccgagaatgg
     7921 agcacagtgc aaaaccaccg atcgtaagag acatgttagc taagttgctt ggcgacgtgc
     7981 cgcgctgtga gttatttagc cgcgacaaag tgaccgggtg ggatatgtgg ggaaatcagt
     8041 gcgactccga ttttgaactt gctcccggca tggcgattaa accatgcaaa atggtgatcg
     8101 catgaagcac tgttatcgat gtggagagcg aaaggaagac gatcgctttc gaccaggaca
     8161 gccttactgg aatcgatggt gtctccggtg tgaaagaaca ccaacagggg tgttaccact
     8221 accgcaggaa aaggaggacg tgtggcgaga cagcgacgaa gtatcaccga cataatctgt
     8281 gaaactgcaa ataccttcca acgaaacgct ccagaaataa acgcaagcca atcccaaaag
     8341 aatctgacgt aaaaaccttc aactacacgg ctcacctgtg ggatatccgg tggctaagac
     8401 atcgtgcgag gaaatgacaa tgcttttaat tcaacctgga tttggactta gcatcaaaaa
     8461 aggccacatg tttggcgaga aagagtcaca acgaaaaatg atgtctatcc ggttgccatt
     8521 tatcagtatt tattggctaa acagggaggc aacaaattat tggtatacat gcgccagagc
     8581 agcatttaac gaccctgact ggtttgtgga aaaccaccac gcagttcgtc aggcaaagag
     8641 aaaggccaac atgacataca tgaaggcgta tcgaaaagca tggaaagaac accgcgatcg
     8701 ataccagcaa gatatggaaa agcttgaatc agaaaacatg gaattaagac gaaagcttgg
     8761 tgaagcaaaa cgagacattg atgcttacaa gcgacttttt aatggtgaaa gccatgctta
     8821 gtccatccca atctcttcaa taccagaaag aaagcgtcga gcgggcttta acgtgcgcta
     8881 actgcggtca gaagctgcat gtgctggaag ttcacgtgtg tgagcactgc tgcgcagaac
     8941 tgatgagcga tccgaatagt tcaatgtacg aggaagaaga cgatggccaa accagcgcga
     9001 agacgatgta aaaacgaaga atgtcgggaa tggtttcacc ctgcattcgc taatcagtgg
     9061 tggtgctctc cagagtgtgg aaccaagata gcactcgaac gacgaagtaa agaacgcgaa
     9121 aaagcggaaa aagcagcaga gaagaaacga cgacgagagg agcagaaaca gaaagataaa
     9181 cttaagattc gaaaactcgc cttaaagccc cgcagttact ggattaaaca agcccaacaa
     9241 gccgtaaacg ccttcatcag agaaagagac cgcgacttac catgtatctc gtgcggaacg
     9301 ctcacgtctg ctcagtggga tgccggacat taccggacaa ctgctgcggc acctcaactc
     9361 cgatttgatg aacgcaatat tcacaagcaa tgcgtggtgt gcaaccagca caaaagcgga
     9421 aatctcgttc cgtatcgcgt cgaactgatt aaccgcatcg ggcaggaagc agtagacgaa
     9481 atcgaatcaa accataaccg ccatcgctgg actgtcgtag agtgcaaggc gatcaaggca
     9541 gagtaccaac agaaactcaa agacctgcga aatagcagaa gtgaggccgc atgacgttct
     9601 cagtaaaaac cattccagac atgctcgttg aagcatacgg aaaccagaca gaagtagcac
     9661 gcagactgaa atgtagtcgc ggtacggtca gaaaatacgt tgatgataaa gacgggaaaa
     9721 tgcacgccat cgtcaacgac gttctcatgg ttcatcgcgg atggagtgaa agagatgcgc
     9781 tattacgaaa aaattgatgg cagcaaatac cgaaatattt gggtagttgg cgatctgcac
     9841 ggatgctaca cgaacctgat gaaaaaactg gagacgatag gattcgacac caaaaaagac
     9901 ctgcttatct cggttggcga tttggtcgat cgcggtacag agaacgtcga atgcctggaa
     9961 ttaatcacat tcccctggtt cagagctgta cgtggaaacc atgagcaaat gatgattgat
    10021 ggcttatcag agcgtggaaa cgtcaatcac tggctactta atggcggcgg ctggttcttt
    10081 aatatcgatt acgacaaaga aattctggct aaagctcttt cccataaagc agatgaactt
    10141 ccgttaatca tcgaactggt gagcaaagat aaaaaatatg tcatctgcca cgccgattat
    10201 ccttgtgacg aatacgagtt tggaaagcca gttgatcatc agcaggtaat ctggaaccgc
    10261 gaacgaatca gcaactcaca agacgggatc gtgaaagaaa tcaaaggcgc ggacacgttc
    10321 atctttggtc atacgccagc agtgaaacca ctcaaatttg ccaaccagat gtatatcgat
    10381 accggcgcag tgttctgcgg aaacctcaca ttgattcagg tacagggaga aggcgcatga
    10441 gactcgaaag cgtagctaaa tttcattcgc caaaaagccc gatgatgagt gactcaccac
    10501 gggccacggc ttctgactct ctttccggta ctgatgtgat ggctgctatg gggatggcgc
    10561 aatcacaagc cggattcgga atggctgcat tctgcggtaa gcatgaactc agccagaacg
    10621 acaaacaaaa ggctatcaac tatctgatgc aatttgcaca caaggtatcg gggaaatacc
    10681 gtggtgtggc aaagctcgaa ggaaatacta aggcaaaggt actgcaagtg ctcgcaacat
    10741 tcgcttatgc ggattattgc cgtagtgccg cgacgcctgg cgcaagatgc agagattgcc
    10801 acggtacagg tcgtgcggtt gatatagcca aaacagagca gtgggggaga gttgtcgaga
    10861 aagagtgcgg aagatgcaaa ggcgtcggct attcaaggat gccagcaagc gcagcatatc
    10921 gcgctgtaac gatgctaatc ccaaacctta cccaacccac ctggtcacgc actgttaagc
    10981 cgctgtatga cgctctggtg gtgcaatgcc acaaggaaga gtcaatcgca gacaacattt
    11041 tgaatgcggt cacacgttag cagcatgatt gccacggatg gcaacatatt aacggcatga
    11101 tattgactta ttgaataaaa ttgggtaaat ttgactcaac gatggataaa tgcactcgtt
    11161 aaataaagcc ctgagttaat agctcggggc tttttgcgtt ttaagcacgg cctttctgaa
    11221 agcacatcaa gccaaatacc agacagacaa aaataatcac cttatccgct tggctacggt
    11281 gcggtgtgct ttgcataaaa gaaaaccagc gcaatggctg gcttcgtgaa agcgggtggc
    11341 aagaggctgc gctaacaacc tcatgccgtt ttgcccgtgc atatcggtca cgaacaaatc
    11401 tgattactaa acacagtagc ctggatttgt tctatcagta atcgacctta ttcctaatta
    11461 aatagagcaa atccccttat tgggggtaag acatgaagat gccagaaaaa catgacctgt
    11521 tagccgccat tctcgcggca aaggaacaag gcatcggggc aatccttgcg tttgcaatgg
    11581 cgtaccttcg cggcagatat aatggcggtg cgtttacaaa aacagtaatc gacgcaacga
    11641 tgtgcgccat tatcgcctgg ttcattcgtg accttctcga cttcgccgga ctaagtagca
    11701 atctcgctta tataacgagc gtgttcatcg gctacatcgg tactgactcg attggttcgc
    11761 ttatcaaacg cttcgctgct aaaaaagccg gagtagaaga tggtggaaat caataatcaa
    11821 cgtaaggcgt tcctcgatat gctggcgtgg tcagagggaa cagataacgg acgacagaaa
    11881 accagaaatc atggttatga cgtcattgta ggcggagagc tatttactga ttactccgat
    11941 caccctcgca aacttgtcac gctaaaccca aaactcaaat caacagcagc cgggcgctat
    12001 cagcttcttt cccgttggtg ggatgcctac cgtaagcagc ttggcctgaa agacttctct
    12061 ccgaaaagcc aggacgctgt ggcattgcaa cagattaaag agcgtggcgc tttgccgatg
    12121 attgatcgcg gtgatatccg tcaggcaatc gaccgttgca gcaatatctg ggcttcgttg
    12181 cccggggctg gttatggcca gttcgagcat aaggctgaca gcctgattgc aaaattcaaa
    12241 gaagcaggcg gaacggtcag agagattgag gtatgagcag agtaaccgcg attatctccg
    12301 ctctggttat ctgcatcatc gtctgcctgt catgggctgt taatcattac cgtgataacg
    12361 ccatcgccta caaagagcag cgcgacaaag ccgcatccac tatcgctgat atgcagaagc
    12421 gtcaacgtga cgtagcagaa ctcgacgcca gatatacaaa ggagcttgct gatgctaacg
    12481 cgactatcga aagcctccgt gctgatgttt ctgctggtcg taagcgcctg caagtcgccg
    12541 ccacctgtgc aaagtcaacg accggagcca gcagcatggg cgatggagaa agcccaagac
    12601 ttacagcaga tgctgaactc aattattacc gtctccgaag cggaatcgac aggataaccg
    12661 cgcaggttaa ctacctgcag gaatacatca ggactcagtg cctgaaataa tttttttgca
    12721 aatcacaaag tccatttaat gagcctcgcg atgcggggct tttttacatc tgaatttcac
    12781 agcgcatctc acgcgcatat tacatcaccc gagcctttca gaaagttgag cctgagaact
    12841 gccgtatatg gtggcgacca tctcgggacg gcttttctgt gagacaggct cactttctaa
    12901 aaggtaaaga cgcaatgaac tacccaaccg ttgttaacga tatagatttc agagacctaa
    12961 tttttgtagc aaacaacgat ccggttacag attcttttat ggtggcaaaa gcatttggaa
    13021 agctgccgaa gaacgtagtt cgtgacattg agcgaaccat agaagcttgc cctcctgagt
    13081 ttgatacaaa gctcaacttt gagctttgct ataaaaacaa tgagttacag aatggtaagc
    13141 cgcaaaaatt ctaccgtctc cgcaaggatg ggttgatgct tttggttatg tcctacacca
    13201 aaaaagaagc aatgcgtatc aaaattgctt acatcaacgc atttaactgg atgtacgcca
    13261 tgcttcaggt tggtcatcgt caatttgaag aagagagaaa tgccgtaatg ctggagtaca
    13321 tgaaagagaa ggatgttgcc agcatgtcag gtcgcctgct aaatcgctgg ggcaaaatta
    13381 agaagccaca gctgatggct agaattgaac gccttgaaca gcacgggcaa accgtaatcc
    13441 ccggactcat caattaacgg cagcaccgcg aaacaaccca agccagtaag tggggaaata
    13501 acactggcag ccactgaaag atgaacctcc agccttatgg caaaaaagat tctttgtggt
    13561 ggcggactga tggaaagaca tcggatagaa tcaagcagtg gctagggtag ctcccgaaaa
    13621 gcggactcgt caccgcctgc cactgaatct atggcgaaca actagacgag gttgtgatgg
    13681 atgataacgg aaaccaatgg gtcaatgttg aacaccggct accagaaagc aaagaaggga
    13741 tgtggtctaa agaggttata gctcttactg atactggtga cgtgttcaaa ctatcatgta
    13801 tgggctctta ctggcagaga accaaggcat tcatcgattc aggagcaagc aaggttacac
    13861 actggatgcc gcttaattac ccagatgatt aaaacggatg aaaaatgaaa ggtcgctcag
    13921 gcggcctttt ttattgccat cacaaaagcc attccttact gagtggcttt gataatggct
    13981 tataccctac acgggataac ttaactgata tcccttttaa cggataaatg gagccaacaa
    14041 tggcagagat tattcccatg actgaagaac agaaattcca gttagagatt tacaaactgg
    14101 tcatgaacca gaacgcagcc gcagaagaag catttcaatt cattggcact gacgaactga
    14161 agcttgagct attcaaaatt cacttccagt caggtggcgc taattcagat atcacgatcc
    14221 gcacatttga agcggtgcgt aaatcgaagg aagcgttaga cctgttcacc accggagcat
    14281 gatgctcaac ctgaaataac gattaagtga gatgaatatg gcagcaccaa agggcaaccg
    14341 attctgggag gcccgcagta gtcatgggcg aaaccctaaa ttcgaatcgc ctgaggcgct
    14401 gtgggctgct tgttgtgaat acttcgagtg ggctgatgat aacccgctat gggagggtaa
    14461 ggtattttca tatcagggag aaataattaa ggctaatgtc cctaagatgc gagccatgac
    14521 tatttcagga ttgtgtacct tccttgatat caccaggcaa acatggggaa ccttccggtc
    14581 aatggaaggt ttttctgacg tcacatcacg agcggaagac atcatctacg accagaaatt
    14641 ctctggcgca gccgctgacc ttctcaacgc taacatcatc gcccgtgatt tgggcctcaa
    14701 agagcagtcg caagttgaag acgtgacacc tgataaggga gatcgcgata agcggcgctc
    14761 tcgtatcaag gagctattca accgtggaac tggacgcgat tcttgataac ttgagcgacg
    14821 aagagcaaat cgaattgctc gagctactcg aagaagaaga gaactaccgt aacacacacc
    14881 tgctatatga atttacgcca tacagcaaac agcgtgagtt catcgacgcc gggcatgact
    14941 atccagagcg ctgttttatg gctggtaacc agcttggtaa gtcatttact ggtgctgctg
    15001 aagtcgcgtt tcaccttacc gggcgttatc cgggcacaaa aggctatcct gctgatggta
    15061 aatatggcgg tgagtggaaa ggtaagcgtt tttatgagcc tgttgtcttt tggattggtg
    15121 gcgagacaaa cgagactgta accaaaacga ctcaacgcat cctgtgtggt cgtatcgaag
    15181 agaatggtga gccaggctac ggttccatac ctaaagaaga catcattagc tggaagaagt
    15241 ctcctttctt tccgaacctt gttgatcacc ttctggttaa gcatcacacg gctgatggcg
    15301 ttgaagatgg catttcaatc tgctacttca agccatactc gcaaggtcgt gctcgctggc
    15361 agggtgacac aatccacggc gtgtggtttg acgaagagcc accatacagc atttatggtg
    15421 aaggccttac ccgtaccaac aaatacgggc aattctcaat tctgacgttt accccgctga
    15481 tggggatgtc tgacgttgtt accaagttcc tgaagaatcc aagcaagtcg cagaaagtgg
    15541 tcaacatgac catctacgac gctgagcact acacagacga acagaaagag caaatcatcg
    15601 catcttatcc tgagcatgag agagaggcgc gtgctcgcgg tattcctacg atgggtagtg
    15661 gtcgaatctt ccagataccg gaagagacga ttaagtgtca gccgttcgag tgtcctgatc
    15721 acttctacgt aattggcggg atggatttcg gatgggatca cccgcaggcg caggttcagc
    15781 tttggtggga caaggacgca gacacaatat acgtttcacg cgtgtggaag gcgaaagaaa
    15841 aaacagccgt tcaggcgtgg ggagccgtta aatcatgggc gcataaagtg ccaactgcat
    15901 ggcctcatga cggaaaccag cacgagaagg gcggcggtga gcagctcaaa gggcagtatg
    15961 cagatgctgg ttttatgatg ttgcaggagc atgcgacatg gcctgatggc ggtaatgctg
    16021 tggagcctgg catcactgaa ttgcgcgaca tgatgctcga tggtcgcttc aaagtattca
    16081 acacctgtga gccattcttt gaggagttcc gcctctatca ccgtgatgaa aacgggaaaa
    16141 tcgtcaagct taacgacgac gttctctcag ccgttcgcta tgcatacatg atgcgccgct
    16201 tcgc
//
LOCUS       DAJTQQ010000084        16125 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000084 DAJTQQ010000000
VERSION     DAJTQQ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16125
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.084"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..1809)
                     /gene="speA"
                     /locus_tag="OO309_003019"
     CDS             complement(<1..1809)
                     /gene="speA"
                     /locus_tag="OO309_003019"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR5848081.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEG"
     gene            complement(1818..1949)
                     /gene="yqgB"
                     /locus_tag="OO309_003020"
     CDS             complement(1818..1949)
                     /gene="yqgB"
                     /locus_tag="OO309_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR5848082.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2085..2300
                     /locus_tag="OO309_003021"
     CDS             2085..2300
                     /locus_tag="OO309_003021"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848083.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2604..3758
                     /gene="metK"
                     /locus_tag="OO309_003022"
     CDS             2604..3758
                     /gene="metK"
                     /locus_tag="OO309_003022"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR5848084.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4195..5589
                     /gene="galP"
                     /locus_tag="OO309_003023"
     CDS             4195..5589
                     /gene="galP"
                     /locus_tag="OO309_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR5848085.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5666..6163
                     /locus_tag="OO309_003024"
     CDS             5666..6163
                     /locus_tag="OO309_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR5848086.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6258..6965
                     /gene="endA"
                     /locus_tag="OO309_003025"
     CDS             6258..6965
                     /gene="endA"
                     /locus_tag="OO309_003025"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR5848087.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7045..7776
                     /gene="rsmE"
                     /locus_tag="OO309_003026"
     CDS             7045..7776
                     /gene="rsmE"
                     /locus_tag="OO309_003026"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR5848088.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            7789..8739
                     /gene="gshB"
                     /locus_tag="OO309_003027"
     CDS             7789..8739
                     /gene="gshB"
                     /locus_tag="OO309_003027"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR5848089.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            8848..9411
                     /locus_tag="OO309_003028"
     CDS             8848..9411
                     /locus_tag="OO309_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR5848090.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9411..9827
                     /gene="ruvX"
                     /locus_tag="OO309_003029"
     CDS             9411..9827
                     /gene="ruvX"
                     /locus_tag="OO309_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR5848091.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARFGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10003..10983)
                     /locus_tag="OO309_003030"
     CDS             complement(10003..10983)
                     /locus_tag="OO309_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR5848092.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            11001..11705
                     /gene="yggS"
                     /locus_tag="OO309_003031"
     CDS             11001..11705
                     /gene="yggS"
                     /locus_tag="OO309_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR5848093.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11723..12289
                     /gene="yggT"
                     /locus_tag="OO309_003032"
     CDS             11723..12289
                     /gene="yggT"
                     /locus_tag="OO309_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR5848094.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12286..12576
                     /locus_tag="OO309_003033"
     CDS             12286..12576
                     /locus_tag="OO309_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR5848095.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12584..13177
                     /locus_tag="OO309_003034"
     CDS             12584..13177
                     /locus_tag="OO309_003034"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR5848096.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13170..14306
                     /gene="hemW"
                     /locus_tag="OO309_003035"
     CDS             13170..14306
                     /gene="hemW"
                     /locus_tag="OO309_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR5848097.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14620..15583
                     /locus_tag="OO309_003036"
                     /pseudo
     CDS             14620..15583
                     /locus_tag="OO309_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15628..>16125
                     /locus_tag="OO309_003037"
     CDS             15628..>16125
                     /locus_tag="OO309_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR5848098.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEA"
BASE COUNT         3978 a         4164 c         4070 g         3913 t
ORIGIN      
        1 gccttcgtca gacagttcta cttctacgct accgtcaggg aacacgaaca cgtcaactgc
       61 ttcggtatca ccgaacaggt tgtgcatatt gccaaggatc tcctgatatg caccgaccat
      121 aaagaaaccg agcatcggcg gattctctgg atcgtactcc ggcattggca ttgtcgtggc
      181 aataccatca ccatcaatat agtggtcgat agcaccgtca gagtcacagg taatatccag
      241 cagcacagcg cgacgttccg gcacttgatc cagcccttcc agcggtagaa ccgggaacaa
      301 ctggtcgatc ccccatgcgt ccggcattga ctggaacagc gagaaattga cgtacatttt
      361 gtccgccata cgttcctgca gttcgtcgat tatcggacga tgcgcacggt tttgcggatc
      421 cagttgcttc tgcacttcat ggcacatgct cagatagagt tgttctgccc atgcacgttc
      481 ttgcaggcta aaggtgccgg aagagtagcc gatatgaatg tcgtgcagat ccatctggct
      541 gtcgtgtaac cattcacgca aagaacggcg agttcccggt tcgtgcattt cctgccaggt
      601 ttcccacatg ctttgcagcg cacgcggcgc atcttctacg ggcgcggtcg gcaccgtata
      661 ttcgttacgt tccacgccga tgatattcga caccagcacg gtatggtgcg cagtcaccgc
      721 acggcccgat tcggtgatta ccgtcggatg cggcagacca ttttcttcac acgcatcgcc
      781 aatcgcccag atgatgttgt tggcgtattc attgaggcca tagtttacgg agcagtcaga
      841 ctgcgaacgg gtgccttcat aatccacgcc cagaccgccg ccgacgtcga agcactgaat
      901 attgacgccc agcttgtgca gttccacata gaaacgcgcg gattcacgaa cgcctgtcgc
      961 gatatcgcga atattcgcca tctgcgaacc gaggtggaag tgcagtagtt gcaggctgtc
     1021 gagacgcccg gcttcacgca gggtttcaac cagttgcagt acctgagtcg cagccaggcc
     1081 gaacttcgat ttttccccgc cggaggactg ccatttaccc gagccctgcg aagccagacg
     1141 tgcacgcacg cccagacgag gaacgacatt cagacgttct gcttcatcca gcacaatggc
     1201 gatttctgac atcttctcaa tgaccagata gaccttgtgc cccatcttct cgccaattaa
     1261 tgccaggcgg atatattcgc ggtctttata accgttgcag acgatgacgc tacgagtcat
     1321 gccagcatgt gccagcactg ccatcaactc ggctttggaa ccggcttcca gacccagcgg
     1381 ttcacccgaa tgaatcaggg actcgatcac gcggcggtgc tggttaactt tgatcggata
     1441 aacaaggaag taatcgccgt tatagccgta ggattccctc gcacgtttga acgcggcgtt
     1501 aatggaacgc aaacggtgct gcaggatctg tgggaaacag aacagtgcag gcagacgctg
     1561 accctgtgct tcacgagttt tcactaactg cgcgagatcg acgcgagctt ccgggacgtc
     1621 cgggtccggg cacacgctga tgtggcccag ctcgttaacg tcatagtagt tattgcccca
     1681 ccaggcaata ttgtaagtac gcagcatctt gctggcttcc tgggagctca ttgcaacctc
     1741 ctgcatggag cgtagtacac cgtgttcgcc cgctgacgaa ggcaaaccca tagacatgtc
     1801 gtcagacata gcgaacctca aattatttta ttaagtgtaa aacagttaac gactgtcgca
     1861 gcctggaact gcgataacaa cccataagca catggatttt ccagcagtgt atgctgacgc
     1921 tccgactgcg caaccggttt ctttttcata acattattaa gcacataacc gaacgtaagt
     1981 gtgacagttc ggcgaaacca cgagaaaact cttgttttta caagagcgcc cttgttcagt
     2041 cctcagtaac tgtaaccagc tcttgaatcc tgagaagcgc cgagatgggt ataacatcgg
     2101 caggtatgca aagcagagat gcagagtgcg gggaacgagt cttcaccaga acggtgagac
     2161 aggttaagca gcagacaacg gttcattatt tcgtatcacc tccacggccg cctgttaaga
     2221 cgaacccaca agccaaaact ctgatttcaa cccggctgga agtggcaaca cgaaagaaac
     2281 gtcgtgtgct ttttatttaa gccgcgcgcc gcgttttata ccccacaatg gcagaaaatt
     2341 gcaaaagata aatacgcaga atgccggcat tgtcaggaaa aatttccagc cacgttttta
     2401 acagaatgag acacgattca aaaaaaagtg gaaatcgggt gaagaattga cctaaaatag
     2461 ccatccagat gttaatccat ccataccgat taacactcag actgccagtg tttttaacct
     2521 gcagagtcgt ggtaggatcc gctaccacag aaaatccaca caacagtttg agctaaccaa
     2581 attctcttta ggtgatatta aatatggcaa aacacctttt tacgtccgag tccgtctctg
     2641 aagggcatcc tgacaaaatt gctgaccaaa tctctgatgc cgttttagac gcgatcctcg
     2701 aacaggatcc gaaagcacgc gttgcttgcg aaacctacgt aaaaaccggc atggttttag
     2761 ttggcggcga aatcaccacc agcgcctggg tagacatcga agagatcacc cgtaacaccg
     2821 ttcgcgaaat tggctatgtg cattccgaca tgggctttga cgctaactcc tgtgcggttc
     2881 tgagcgctat cggcaaacag tctcctgaca tcaaccaagg cgttgaccgt gccgatccgc
     2941 tggaacaggg cgcgggtgac cagggtctga tgtttggcta cgcaactaat gaaaccgacg
     3001 tgctgatgcc agcacctatc acctatgcac accgtctggt acagcgtcag gctgaagtgc
     3061 gtaaaaacgg cactctgccg tggctgcgcc cggacgcgaa aagccaggtg actttccagt
     3121 atgacgacgg caaaatcgtt ggtattgatg ctgtcgtgct ttccactcag cactctgaag
     3181 agatcgacca gaaatcgctg caagaagcgg taatggaaga gatcatcaag ccaattctgc
     3241 ccgctgaatg gctgacttct gccaccaaat tcttcatcaa cccgaccggt cgtttcgtta
     3301 tcggtggccc aatgggtgac tgcggtctga ctggccgtaa aattatcgtt gatacctacg
     3361 gcggcatggc gcgtcacggt ggcggtgcat tctctggtaa agatccatca aaagtggacc
     3421 gttccgcagc ctacgcagca cgttatgtcg cgaaaaacat cgttgctgct ggcctggccg
     3481 atcgttgtga aattcaggtt tcctacgcaa tcggcgtggc tgaaccgacc tccatcatgg
     3541 tagaaacttt cggtactgag aaagtgcctt ctgaacaact gactctgctg gtacgtgagt
     3601 tcttcgacct gcgcccatac ggactgattc agatgctgga tctgctgcac ccgatctaca
     3661 aagaaaccgc agcatacggt cactttggtc gtgaacattt cccgtgggaa aaaaccgaca
     3721 aagcgcagct gctgcgcgat gctgccggtc tgaagtaatc tttcttcacc tgcgttcaaa
     3781 ggccagcctc gcgctggcct ttttctttta cctcaccgcg gtggataggc gttcacgccg
     3841 catccggcaa aaaaactgcc cgcacaataa catcattctt cctgatcacg tttcacggca
     3901 aattaccatc acaactgaaa ccgattacac caaccacacc agcaaaatat ttgtaatatt
     3961 ttcatattat tattcagctt tcacagttgt tacatttctt ttcagtaaag tcttaattgc
     4021 agatgacagc gtttaatcta tgatgatata actcaattat tttcatgccc ttaaatcata
     4081 actaagataa atgttagtgt aagcgattac actgatgtga tttgcttcac atctttttac
     4141 gtcgtactca cctatcttaa ttcacaataa aaaataacta tattggaggg catcatgcct
     4201 gacgctaaaa aacaggggcg gtcaaacaag gcaatgacgt ttttcgtctg cttccttgcc
     4261 gctctggcgg gattactctt tggcctggat atcggtgtaa ttgctggcgc actgccgttt
     4321 attgcagatg aattccagat aacttcgcac acgcaagaat gggtcgtaag ctccatgatg
     4381 ttcggtgcgg cagtcggtgc ggtgggcatc ggctggctct cctttaaact cgggcgcaaa
     4441 aagagcctga tgatcggcgc aatcctgttt gttgccggtt cactgttctc tgcggctgcg
     4501 ccaaacgttg aagtactgat tctttcgcgc gttctgctgg ggctggcggt gggtgtggcc
     4561 tcttataccg caccactgta cctgtcagaa attgcgccgg aaaaaattcg cggcagtatg
     4621 atctcgatgt atcagttgat gatcactatc gggatcctcg gtgcttatct ttctgatacc
     4681 gccttcagct acaccggtgc atggcgctgg atgctgggtg tgattatcat cccggcaatt
     4741 ttgctgctga ttggtgtctt cttcctgcca gacagcccac gttggtttgc cgccaaacgc
     4801 cgttttgttg atgccgaacg cgtgctgcta cgcctgcgtg acaccagcgc ggaagcgaaa
     4861 cgcgaactgg atgaaatccg tgaaagtttg caggttaaac agagtggctg ggcgctgttt
     4921 aaagagaata gcaacttccg ccgcgcggtg ttccttggcg tactgttaca ggtaatgcag
     4981 caattcaccg ggatgaacgt catcatgtat tacgcgccga aaatcttcga actggcgggt
     5041 tataccaaca ccaccgagca aatgtggggg acagtgattg tcggcctgac caacgtactt
     5101 gccaccttta tcgcaatcgg ccttgttgac cgctggggac gtaaaccaac gctaacgctg
     5161 ggcttcctgg tgatggctgc tggcatgggc gtactcggta caatgatgca tatcggtatc
     5221 cactctccgt cggcgcagta tttcgccatc gccatgctgc tgatgtttat tgtcggtttt
     5281 gccatgagtg ccggtccgct gatttgggta ctgtgctccg aaattcagcc gctgaaaggc
     5341 cgcgattttg gcatcacctg ctccaccgcc accaactgga ttgccaacat gatcgttggc
     5401 gcaacgttcc tgaccatgct caacacgctg ggtaacgcca acaccttctg ggtgtatgcg
     5461 gctctgaacg tactgtttat cctgctgaca ttgtggctgg taccggaaac caaacacgtt
     5521 tcgctggaac atattgaacg taatctgatg aaaggtcgta aactgcgcga aatcggcgct
     5581 cacgattaat ctcctcaggc ttcctcccgt cgcggaggaa gccgcctctt gcagtcatct
     5641 tttcttcgct ctatcctctg ccgctatgaa aacatcccgt ctccctatcg ccatccaaca
     5701 ggccgttatg cgccgcctgc gggaaaaact cgcccaggcc aacctgaagc tagggcgtaa
     5761 ctacccggag ccaaaactct cttacaccca gcgcggaacc tccgccggaa cagcctggct
     5821 ggaaagctat gaaatccgcc tcaatcccgt tttgctgttg gaaaacagtg aagcttttat
     5881 tgaagaagtg gtaccgcacg aactggcaca tttgctggta tggaaacatt tcggccgcgt
     5941 agcgccacat ggcaaagagt ggaagtggat gatggaaagc gtgctgggcg ttcccgcccg
     6001 tcgtacgcat cagttcgaac tgcaatccgt gcgtcgcaac accttcccct accgctgtaa
     6061 gtgccaggaa catcagctta ccgtacgccg ccataatcgc gtagttcgtg gcgaggccat
     6121 ctatcgctgt gttcactgcg gtgaacagct ggttgcgaaa taaccacctg aactatcagg
     6181 aactttcctg atctggctga ttgcatacca aaacaacttt cgctacgttg ctggctcgtt
     6241 ttaacacgga gtaagtgatg taccgttatt tgtctattgc tgcggtggta ctgagcgcag
     6301 cattttccgg cccgacgttg gccgaaggta tcaatagttt ttctcaggcg aaagccgcgg
     6361 cggtaaaagt ccacgctgat gcgcccggta cgttttattg cggatgtaaa attaactggc
     6421 agggcaaaaa aggcgttgtt gatctgcaat cgtgcggcta tcaggtgcgc aaaaatgaaa
     6481 accgcgccag ccgcgtagag tgggaacacg tcgttcccgc ctggcaattc ggtcaccagc
     6541 gccagtgctg gcaggacggt ggacgtaaaa actgcgctaa agatccggtc tatcgcaaga
     6601 tggaaagcga tatgcataac ctgcagccgt cagtcggcga ggtgaatggc gatcgcggca
     6661 attttatgta cagccagtgg aatggcggcg aaggccagta cggtcaatgc gccatgaagg
     6721 tcgatttcaa agaaaaagct gccgaaccac cagcgcgtgc acgcggtgcc attgcgcgca
     6781 cctacttcta tatgcgcgac caatacaacc tgacactctc tcgccagcaa acgcagctgt
     6841 tcaacgcatg gaacaagatg tatccggtta ccgactggga gtgcgagcgc gatgaacgca
     6901 tcgcgaaggt gcagggcaat cataacccgt atgtgcaacg cgcttgccag gcgcgaaaga
     6961 gctaacctac actagcggga ttctttttgt taacccctac cccacgcgta caaccgcgtg
     7021 gggagacgac gcggattttt aactatgcgt atcccccgca tttatcatcc tgaaccactg
     7081 accagccatt ctcacatcgc gctttgcgaa gatgccgcca accatatcgg gcgcgtactg
     7141 cgcatggggc cggggcaggc gttgcaattg tttgacggta gcaaccaggt ctttgacgcc
     7201 gaaattacca gcgccagcaa aaaaagcgtg gaagtgaagg tgctggaagg ccagatcgac
     7261 gatcgcgaat ctccgctgca tattcacctc ggtcaggtga tgtcgcgtgg tgaaaaaatg
     7321 gaatttacta tccagaaatc gatcgaactc ggtgtaagcc tcattacgcc acttttttct
     7381 gagcgctgcg gcgttaaact ggatagtgaa cgtctgaaca agaagcttca gcagtggcag
     7441 aagattgcaa ttgctgcctg tgagcagtgt ggtcgtaacc gggtgccgga aatccgtcca
     7501 gcgatggatc tggaagcctg gtgtgcagag caggatgaag gactgaaact gaatcttcac
     7561 ccgcgcgcca gtaacagcat caatacgttg cctttaccgg ttgaacgcgt ccgcctgctg
     7621 attggcccgg aaggcggttt atcggcagat gaaattgcca tgactgcccg ctatcaattt
     7681 actgatatcc tgttgggacc tcgcgttttg cgtacagaga caactgcgct caccgccatt
     7741 accgcgctac aagtacgatt tggcgatttg ggctaacgga gaagaataat gatcaagctc
     7801 ggcatcgtga tggaccccat cgcaaacatc aacatcaaga aagattccag tttcgctatg
     7861 ttgctggaag cacagcgtcg tggttacgaa cttcactata tggagatggg cgatctgtat
     7921 ctgatcaatg gcgaagcccg cgcccatacc cgcacgctga acgtgaagca gaactacgaa
     7981 gagtggtttt cgttcgtcgg tgaacaggat ctgccgctgg ccgatctcga tgtgatcctg
     8041 atgcgtaaag acccgccgtt tgataccgag tttatctacg cgacctatat tctggaacgt
     8101 gccgaagaga aagggacgct gatcgttaac aagccgcaga gcctgcgcga ctgtaacgag
     8161 aaactgttta ccgcctggtt ctctgactta acgccagaaa cgctggttac gcgcaataaa
     8221 gcgcagctaa aagcgttctg ggagaaacac agcgacatca ttcttaagcc gctggacggt
     8281 atgggcggcg cgtcgatttt ccgcgtgaaa gaaggcgatc caaacctcgg cgtgattgcc
     8341 gaaaccctga ctgagcatgg cacttgctac tgcatggcgc aaaattacct gccagccatt
     8401 aaagatggcg acaaacgcgt gctggtggtg gatggcgagc cggtaccgta ctgcctggcg
     8461 cgtattccgc aggggggcga aacccgtggc aatctggctg ccggtggtcg cggtgaacct
     8521 cgtccgctga cggaaagtga ctggaaaatc gcccgtcaga tcgggccgac gctgaaagaa
     8581 aaagggctga tttttgttgg tctggatatc atcggcgacc gtctgactga aattaacgtc
     8641 accagcccaa cctgtattcg tgagattgaa gcagagtttc cggtgtcgat caccggaatg
     8701 ttaatggatg ccatcgaagc acgtttacag cagcagtaac ccaccttagc gagaaggatc
     8761 tcgttgagac tctgagtgac agcgcccttc tttccacgca tactgggcgc tgttgctttt
     8821 ttgaaccagg aaacagaacc tctgacaatg aatttacagc atcactttct tattgccatg
     8881 cctgctctcc aggatccgat tttccgtcgt tccgtggtct acatttgcga acataatacc
     8941 aatggtgcaa tggggatcat cgtcaacaag ccgctggaaa atctcaaaat tgaagggatt
     9001 ctggaaaagc tgaagatcac gccggagccg cgtgatgaat caatccgtct ggataaaccg
     9061 gttatgctcg gcggcccact ggctgaagat cgcgggttta ttttgcatac tccgccctcc
     9121 aattttgctt ccagcattcg catttcagac aacacggtga tgaccacttc ccgcgatgtg
     9181 ctggaaacgc tcggcaccga taaacaaccg tctgacgtat tggtggctct gggctatgcc
     9241 tcctgggaaa aaggtcaact ggaacaagaa attctcgata acgcgtggct aacggccccg
     9301 gcggatctga atattctgtt caaaacgccg attgccgacc gctggcgcga ggcggcaaaa
     9361 ctgattggtg tggatattct caccatgcct ggtgtggcag gacacgcctg atgagtggaa
     9421 ccttactcgc cttcgacttc ggcaccaaaa gcattggcgt agcggtcggc caacgcatta
     9481 ccggcaccgc tcgccctttg cctgcaatta aagcgcagga cggtacgccg gactggaacc
     9541 tcatcgaacg tttactgaaa gagtggcagc ctgacgaaat catcgtcggt ttgccgctga
     9601 atatggacgg caccgaacag ccgttaaccg ccagagcgcg caaatttgcc aaccgtattc
     9661 atggtcgttt cggtgttgaa gtaaagctcc atgacgagcg tcttagcact gtggaagccc
     9721 gtttcggtct ttttgaacag ggcggctatc gggcgctcaa caaaggcaaa gttgactctg
     9781 cctctgcggt tattattctc gaaagctatt tcgagcaggg atattaaggc gatctaaaag
     9841 cctggcggat agtcgttacc gccatcttgc cggatgcggc gtgaacgcct tatccagcac
     9901 acatctggca gcggctgtag gtctgataag acgcgttagc gtcgcatcag acatttattg
     9961 cctttatagg ccctataaac ttgcgcatcg ggcatggcaa cgtcacaaac gcccttcccc
    10021 cacccgctgc tgataactct gctgaaacgt taacatcccc accttctgcc cggtttgaat
    10081 aacatgcggt aactggtggg ttttcccttc gcgaatcaaa ttccccaccg cgggagtgtt
    10141 aatcagtaat tcaaacagcg ccacgcgtcc ttcctgttta tccacttcca gcttttgtga
    10201 cagcactgcc cgtaaactac ccgcaagttg attacgtacg ggatcttttt cctgcgccgg
    10261 aaatgaatcc accagtcgct caactgcctg cgcggcacca cgtgtatgta aggttgccag
    10321 caccagatgc ccggtttctg ccgccgtcag cgccagacgg attgtctcac tgtcacgtag
    10381 ctctccgagc aaaatcacat caggatcttc ccgcaatgca gcccgcaacc ccgatgcgaa
    10441 cgtcatacag tgcaaaccaa tttcccgctg ctggatcaaa catcgctggc tggcatagag
    10501 atattccaca ggatcttcca gcgtcagaat atgcgcatcg gcatgttgat taagatagcc
    10561 aaccatcgcc gccagcgtgg tagatttgcc actccccgta gcccccgtca ccagaatcag
    10621 gccattctcg ctcttgagta attccggcaa taccggtggt gcaccaagct gttcgagctg
    10681 tggacagtgc gaaggtaaca accgtaatgc cagagaaata ccttgccgtt gcgcgaacgc
    10741 actgccacgc aaccgctggt tttccgccag cgacacggca aaatccagct ggccattttc
    10801 cagcaatatt gcccgctgat cgtcatccag ccactcccgc agtagctctt cgacgtccgg
    10861 cgcatcaaac ggcgcagctt ccattctgcc acgaatgcgc catcgtgcgg gccaggcgct
    10921 gcacaggtgt agatccgaga cgttatgctt tacactaagg gccacaattt cttccatatt
    10981 catactaaga tcctcggaaa atgaacgata ttgcgcataa cctggcacag gtccgggaca
    11041 aaatctcagc ggctgcaacg cgttgcggcc gttctccaga agaaattacg ctgcttgcag
    11101 tcagtaaaac aaaacctgcg agcgccatcg cagaagccat tgatgccggg cagcgtcaat
    11161 ttggtgaaaa ctacgttcag gaaggggtag ataaaattcg ccactttcag gaactgggcg
    11221 taacaggatt agaatggcat tttattggcc cgttgcagtc taataaaagc cgcctagtgg
    11281 cagagcattt cgactggtgt cataccatcg accgtttgcg cattgctacc cgcctcaacg
    11341 agcagcgccc ggcagaactt ccccctctta acgtactgat tcaaattaat attagtgatg
    11401 aaaacagtaa gtccgggatt caactggcag aactggacga gctggcagcg gcggtcgctg
    11461 aactaccgcg tttacgtctg cgcgggctga tggcaatccc tgcgcttgag tcagaatatg
    11521 taaggcagtt tgaagttgca cgccaaatgg ctgtagcatt tgccggactg aaaacgcgct
    11581 acccgcatat cgacacgctc tctctgggaa tgtcggacga tatggaagcc gccattgcgg
    11641 caggtagcac gatggttcgt atcggcactg caatttttgg tgcgcgtgat tactctaaaa
    11701 aataaggaat taaaggaacg ccatgaatac gttgactttc ctgctttcaa cggtcattga
    11761 gctgtataca atggtgctgt tattacgcat ctggatgcag tgtgctcatt gtgattttta
    11821 cacccccttc tcacagtttg tagtgaaggt agcgcagcca attatcgggc cactgcgccg
    11881 cgttattccg gcaatgggtc ccattgacag cgcctcgctg ctggttgcct atattctcag
    11941 ttttatcaaa gccattgtgc tgtttgaagt ggtgaccttc ctgccaatca tctggattgc
    12001 cggtttactg attctgctga aaaccatcgg cttgctgatt ttctgggtcc tgctggtgat
    12061 ggcgattatg agctgggtaa gccagggtcg tagcccgatt gaatacgtgc tgattcagct
    12121 ggccgatccg ctgctgcgcc cgattcgccg cttgctaccg gcaatgggtg ggattgattt
    12181 ctcgccgatg atcctcgttc tgctgctgta tgccatcaat atgggtgtcg cagaagtatt
    12241 acaggcaacc ggaaacatgc tgctgccggg gctgtggatg gcgttatgag tgccgtaaca
    12301 gttaatgatg acggtctggt tttacggctc tatattcagc cgaaagccag ccgtgattct
    12361 attgtcggtt tacatggcga cgaagttaaa gtcgccatta ccgcgccgcc ggttgacggc
    12421 caggccaaca gtcatctggt gaagtttctc ggtaagcaat tccgggttgc caaaagccag
    12481 gtggtgattg aaaaaggcga acttggccgc cacaaacaaa ttaaaatcat taatccgcaa
    12541 caaatcccgc cagaaatcgc ggcgttaatt aattaggtat cctatgcaaa aagttgtcct
    12601 cgcaaccggc aatgccggta aagtgcgtga gctggcgtcg ctgcttagcg actttggtct
    12661 tgatatcgtg gcccaaacag acctcggcgt tgattccgct gaagaaaccg gcctaacctt
    12721 tatcgaaaac gcgattctga aagcgcgcca tgcggcaaaa gtgaccggtt taccggcaat
    12781 tgccgacgac tctggtctgg cggtagatgc gctgggtggc gcgccgggta tttactccgc
    12841 gcgttattcc ggtgaagacg cgaccgatca aaagaatctg caaaaactgt tggaaacact
    12901 gaaagacgta ccggacgacc aacgtcaggc gcgtttccac tgcgtgctgg tatatctgcg
    12961 tcacgcggaa gatccgactc cgctggtatg ccacggcagc tggccgggcg tgattactcg
    13021 cgaaccagcg ggcactggtg gctttggtta tgatccaatc ttcttcgtac cttccgaagg
    13081 gaaaaccgcc gccgaactga cccgcgaaga aaagagcgcc atttcccacc gtggtcaggc
    13141 gttgaaactg ctgctggacg ctttacgtaa tggttaaatt accgccgctg agtctctaca
    13201 ttcacatccc gtggtgcgtg cagaaatgcc cgtactgcga tttcaactct cacgcgttga
    13261 aaggagaagt gccgcacgac gattatgttc agcatctgct ttgcgatctg gacaatgatg
    13321 tggcttacgc tcagggccgt gaagtaaaga caatctttat tggcggtggt acgccgagcc
    13381 tgctttccgg cccggcgatg caaacgctgc tagacggcgt gcgtgcgcgt ttgccgctgg
    13441 cagcggatgc agaaattact atggaagcga acccaggcac ggtagaagcg gatcgctttg
    13501 tcgattatca gcgtgctggt gtaaaccgca tctctattgg tgtgcagagt tttagcgaag
    13561 aaaagctgaa acgacttggg cgtattcatg gcccgcaaga agcgaaacga gcggcaaagc
    13621 tggcgagcgg tttagggtta cgtagcttta accttgattt gatgcatgga ctaccggatc
    13681 aatcactgga agaggcgctt ggcgatctac gccaggccat tgaactgaat ccgccgcatc
    13741 tttcctggta tcaactgact atcgaaccta atacgctgtt tggctcgcga ccgccggtgc
    13801 tgccggacga tgacgcgctg tgggatattt tcgaacaggg gcatcagtta ttaaccgcag
    13861 cgggttatca gcaatatgaa acttccgctt acgccaaacc cggttatcag tgccagcaca
    13921 atctcaacta ctggcgcttt ggtgactaca tcggtattgg ctgcggcgcg cacggcaaag
    13981 tgaccttccc ggatgggcgc attctgcgta ccactaaaac gcgtcatcct cgtggtttta
    14041 tgcaaggaag gtatctggaa agccagcgtg atgtcgaagc cgcagataag ccgtttgagt
    14101 tctttatgaa tcgcttccgt ctgctggagg ccgcgccgcg cgtggagttt attgcgtata
    14161 ccgggctttg cgaagatgtg attcgcccac agttagacga ggcgattgcc cagggttatc
    14221 tcaccgaatg tgcggattac tggcagataa cggaacatgg taaattattc ttaaactcac
    14281 ttcttgaatt attcctcgct gagtaatcat taatgcgcct gcgggcgcat taatcacttc
    14341 ttttatcccc ccgttaatta acctcggcag aaatatattt cttactgaat aaatatcgac
    14401 gacatttatt atatatattt agtgaatatt tactaatcat tcattgatta taaatatgta
    14461 caacctggcg cacaaattta atatttaatt tctgacttta tttcaagttg tgataaacat
    14521 agacaagttg tcagacaggt taaccaattt gtcgcacaaa tcactgataa gcacaattag
    14581 atatcaacca atttccatcc ttcaacaggg agttttttta tgaaaatgag gtcatttacc
    14641 cgctcattag tctgcgcatc cttgctggct ctggttagca ctggcgtaaa cgccgcagaa
    14701 aaagtggcgt taaagttagc atacaacctg gaaagaagtc atgttgtaca tcagagtttt
    14761 gaagagctgg cgaaagaggt aaaacagctg tcaaaaggta atatggtgat ccgtatttat
    14821 cccagcagcc agatgggtaa tgcgcgagaa actatggaat tgttgcaaaa tggcgcactg
    14881 gatatgacca aaggttccgc cagtgatctg gagtcttttg ataatattta tgctatctat
    14941 aatttgccgt tcttattaag ttgtttttgg tgaagttggt aaagaaatta tggactcaac
    15001 aaaagacaaa ggcttttttg ctttatcagc ttatgtagcg ggtactcgca gtttctatgc
    15061 gaaaaaaccg attacaaagc cagaagatct taaaggactg aaaattcgtg ttcagccaag
    15121 tccgaccacc atcaaaatga ttgaactgat gggtggttca cccactccaa tttcatttgg
    15181 cgaagtttat actgcgatgc agcaaggtgt agtagatggc gcagaaaata acgtgccttc
    15241 ctggatgcag actcgacata ttgaaattgc caaagttttt tctgaagatg aacacgcctc
    15301 aattcctgac tttttggtta tctccaccaa aacatggaat aaattaaccc cagaacagca
    15361 acaaatcctc gaaacagcgg cgaagaaatc cgaagcttat caacaaaaat tgtgggaaaa
    15421 aattgacgct gacacacgtg ctcaggcgaa agctatgggg ggtgaaattg taaaagtgga
    15481 taaagcgcca ttccgtaaag ccgtgcaacc tctgtttgac gacttcaaaa aagatccaaa
    15541 acaagctgct ttgttagaga aattcgacaa cgctgctcaa taatattccg ggccagcctc
    15601 tggccctgat tttccaggat gcttacaatg atatttaacc gcctgaaact tgcggtagac
    15661 cgcgtcattg ccacgttttc tgtcgccgta atgcttgctc tggttgtatg cgtagtctgg
    15721 caagtattta gccgctacgt acttaaccag ccaagtacat taaccgatga gctggcacgc
    15781 tttttgatga tctgggtagg attattaggt gctgcatata ccgtcggcgc gcagcgacat
    15841 ctctctattg atttatttgc tctcgccctg aataagcgca aacaattact tcttagtata
    15901 gtcattaacg ttctcatttt aggttttgcc ggttcggtca ttgtgactgg tggcctgaaa
    15961 ttaattgata aaacactggc aacatcacag gtatctgccg ctatgcaaat accgatggga
    16021 tacgtatata tcattctgcc attaagtgga gtggtaatga tgttttatgc cttgtgtttt
    16081 attaatcaaa gcattcaaca actgaaacaa cctgcgcagg aggcc
//
LOCUS       DAJTQQ010000085        16084 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000085 DAJTQQ010000000
VERSION     DAJTQQ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16084
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.085"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..928)
                     /gene="anmK"
                     /locus_tag="OO309_003038"
     CDS             complement(<1..928)
                     /gene="anmK"
                     /locus_tag="OO309_003038"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR5848099.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAV"
     gene            1202..1669
                     /gene="slyB"
                     /locus_tag="OO309_003039"
     CDS             1202..1669
                     /gene="slyB"
                     /locus_tag="OO309_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR5848100.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(1716..2150)
                     /gene="slyA"
                     /locus_tag="OO309_003040"
     CDS             complement(1716..2150)
                     /gene="slyA"
                     /locus_tag="OO309_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR5848101.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2351..2587
                     /locus_tag="OO309_003041"
     CDS             2351..2587
                     /locus_tag="OO309_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR5848102.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2590..3447
                     /locus_tag="OO309_003042"
     CDS             2590..3447
                     /locus_tag="OO309_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5848103.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3447..5459
                     /locus_tag="OO309_003043"
                     /pseudo
     CDS             3447..5459
                     /locus_tag="OO309_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5460..5981)
                     /gene="sodC"
                     /locus_tag="OO309_003044"
     CDS             complement(5460..5981)
                     /gene="sodC"
                     /locus_tag="OO309_003044"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR5848104.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6062..6958)
                     /locus_tag="OO309_003045"
     CDS             complement(6062..6958)
                     /locus_tag="OO309_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR5848105.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7007..7246)
                     /locus_tag="OO309_003046"
     CDS             complement(7007..7246)
                     /locus_tag="OO309_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR5848106.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7349..7948
                     /gene="nemR"
                     /locus_tag="OO309_003047"
     CDS             7349..7948
                     /gene="nemR"
                     /locus_tag="OO309_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR5848107.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            7985..9082
                     /gene="nemA"
                     /locus_tag="OO309_003048"
     CDS             7985..9082
                     /gene="nemA"
                     /locus_tag="OO309_003048"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR5848108.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9163..9570
                     /gene="gloA"
                     /locus_tag="OO309_003049"
     CDS             9163..9570
                     /gene="gloA"
                     /locus_tag="OO309_003049"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR5848109.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            9673..10320
                     /gene="rnt"
                     /locus_tag="OO309_003050"
     CDS             9673..10320
                     /gene="rnt"
                     /locus_tag="OO309_003050"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR5848110.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10413..12527
                     /locus_tag="OO309_003051"
     CDS             10413..12527
                     /locus_tag="OO309_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5848111.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGL
                     SATVRSASDVAAFLGGDRPVTVVNPPTMRHPQIRIVVPVANMDDVSSVASGTGEDSHA
                     GREGSIWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVD
                     AAHFESTSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLEL
                     GIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVEC
                     MFAGRLENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRTAPWKDLPRRVFDAT
                     LDMLSGRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPE
                     GEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSAQLPF
                     WRGEGNGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATG
                     IVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASD
                     DSIVARIPDTDGKLPDAAIFLF"
     gene            12537..13829
                     /locus_tag="OO309_003052"
     CDS             12537..13829
                     /locus_tag="OO309_003052"
                     /inference="COORDINATES: protein motif:HMM:NF020083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848112.1"
                     /translation="MLQIVREAVGSSALFAARFRECAARALLMPGRTPGHRTPLWQQR
                     LRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQISDVTTTTP
                     SPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELLDPQVIRQ
                     VEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLENLLAVKR
                     IFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLRYLRAHAL
                     VTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRSLQAAREA
                     TRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQLAGVGLPA
                     SLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKAG"
     gene            14779..14871
                     /locus_tag="OO309_003053"
     CDS             14779..14871
                     /locus_tag="OO309_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR5848113.1"
                     /translation="MNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(14915..>16084)
                     /locus_tag="OO309_003054"
     CDS             complement(14915..>16084)
                     /locus_tag="OO309_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5848114.1"
                     /translation="GGQQEREMRRFAGACRFVFNRALALQNENHEAGNKYIPYGKMAS
                     WLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPRFKKRGQNAAFRYP
                     QDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWYISIQTESEVSTPV
                     HPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQ
                     KRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNV
                     RAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRCACCGHTAKENRLS
                     QSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         3653 a         4256 c         4217 g         3958 t
ORIGIN      
        1 ataccgccag acgcgccatg agtagcggat tacgactacc tccaccacat accatcaatc
       61 gttcgcagcc accgctcaac aaaacttgtt cagaaatggt cacggcggtg agttctgcca
      121 gtgtggcctg aacatcacgg ggatcaacac ccggaaaatg gcgtaaatgg cgctccagcc
      181 agccgtagtt aaagtattcg cgtccggtgc ttttcggtgc aggttgcgag aaatacgggt
      241 cgctgagcat attttgcagc agtgggagaa taactttacc cgcccgtgcc cactcggcat
      301 ctttatcgta aggtttacct gcctgacgcc agatccaggc atccatcagc atgttaccag
      361 gaccggtatc gtagccgcca accagctgcc caggaatgag cagtgacaga ttggcgatgc
      421 cgccaatatt gagcaccatt cgtcgctcgg ttgggtgagc cagcaaggca tgatggaacg
      481 caggtaccag cggtgcgcct tgtcctccca aggcaatatc gcgacggcga aaatcaccga
      541 caaccgtaat tccggtgcgt gccacaattt gattgttatc gccaatctgt aaagtgtgtg
      601 gtgccacgcc cgtcggttca tgccagacgg tttgaccgtg acagccgatc gcaactatat
      661 ctctcgcctg caggttttgt tccttaagca aggcattgac cgcatcagca aaaagtcgcc
      721 cgagttgagt atcaagctgt ccaaactgcg aaagtgtaag ctgctggccc tggcaaatat
      781 ccagtacagc ctgtttcaga gacaccggga tcggccagct caagcttgcc agctgtgcga
      841 cccggtgttc atcaattgtc gccaacacaa catcaacacc atcaaggctg gtgcctgaca
      901 taacgccaat aaagcggccc gatttcatag ttcatccttt ttcaatctga cgtttgcgca
      961 ccactcaaac ataaactttt cgtgaatacc atgcggaatg accgattttt accgttggta
     1021 gtaaaacatt atcttcaaat caataatcat catgaatgtt ttgtttataa ttggttgatc
     1081 ctactttcat tatgatttgc tcatatttgg tagaacatgt aaccatggat tcacatatgc
     1141 catatacttt gaccatgagg gatgcttgcg tggcgtttca tggtgaacag gagatttttc
     1201 aatgattaaa cgcgtattgg ttgtttcaat ggtaggtctg tctcttgtcg gttgtgttaa
     1261 taacgacacc ctgtcagggg atgtttatac cgcttctgaa gcgaaacaag tacagaatgt
     1321 cagctatggc accatcgtta acgtacgtcc ggtacagatt cagggcggtg atgattccaa
     1381 cgttatcggt gcaattggcg gtgctgttct tggtggtttc ctggggaata ctgttggtgg
     1441 cggaaccggg cgttctctgg ctactgcagc aggcgctgtt gcaggtggcg tagctggtca
     1501 gggcgtacag agtgcaatga acaaaacgca gggtgtcgag ctggaaattc gtaaagacga
     1561 tggtaatacc atcatggtgg tacagaaaca aggcaacact cgtttctctc cgggccaacg
     1621 tgtcgtactg gccagcaatg gcagtcaggt gaccgtttct ccgcgctaaa taagtttgcg
     1681 tgtggtcagg ttactgacca cacgccccct tcatttcacc ctttggcctg taactcaatg
     1741 atattatgct caagttttgc gatgagcgta atcagttgct ccagttcctc tgcggagatg
     1801 ccatgtaata tttccgcgcg ggttttgtta ataacagctt ccatttcgct gatcagtggc
     1861 tctgcctttt ccgtcagttt aatacgttta gcccgacgat cgctggcaca agtttgacgc
     1921 gaaattaacc ctttttcttc cagttggtcc agagtacgga ccagtgatgg ctgctcgatg
     1981 ccaatcgctt ttgccagttg aatttgcgac tggtctggag gtaactgatg gatattgtgt
     2041 aacgtaaccc aatgggtttg tgttaactcc agcggtttca ggcgatggtc tatcagagca
     2101 cgccatatgc gcaccaaccg tgccagatca gaacctagtg gcgattccaa tttcatctcc
     2161 ttataattag cttgctaaga tattatgcgg cttttagaat agtgtgcagc aattgtattg
     2221 ctaaaacaaa tgtattgctg catttggtta ccgtcagaca tatttttcag aaattgcgcg
     2281 taaatttttc gcacttaaag aatatttatt aatctaacgc aatatattcg gtcgtaaagg
     2341 aatctacttt gtgaagttta tgctcaatgc aacaggattg cccttacaag atctggtgtt
     2401 cggtgcgtcc gtctattttc ctccgttttt caaagcattc gcgtttggat tcgtcatctg
     2461 gcttgtcgta caccgcctgc ttcgtggctg gatctacgcc ggcgatatct ggcatccctt
     2521 gttaatggat ttatcgctgt ttgcgatttg cgtttgcctt gctctggcaa tactgattgc
     2581 gtggtaacta tgtcaattaa aacaattaag tatttctcaa caatcattgt agcgatagtt
     2641 gcggttcttg ccggatggtg gctgtggaat tattacatgc aatctccgtg gacacgcgac
     2701 gggaaaatac gcgcagaaca ggtttctatc accccacagg tgtcgggacg tattgttgag
     2761 ctgaatataa aagacaacca actggttaac gcgggggaac ttctgctcac gatcgataaa
     2821 acgccctttc agatcgccga gctgaacgca caggctcagc tggcgaaagc gcaatctgac
     2881 ctcgcaaaag ccaataacga agcgaatcgt cgtcgtcatc tctcacaaaa ttttattttt
     2941 gccgaagaac tcgataccgc taacctcaat gttaaagcga tgcaggccag tgttaatgcc
     3001 gcacaggcga cgctgaaaca gacacaatgg caactggcgc aaacggaaat tcgcgccccg
     3061 gtgagtggat gggtgactaa cctcactacc cgcatcggcg attacgctga caccgggaaa
     3121 ccgctgtttg cccttgtcga cagccactcg ttttacgtca ttggttattt tgaagaaacc
     3181 aagttgcgcc atatccgcga aggtgcacca gcacaaatta ccctctatag tgataacaaa
     3241 acgttacagg gtcacgtttc cagtatcggt cgggcgattt atgatcagag cgttgaaagt
     3301 gactcaagcc ttattccgga tgtgaaacct aacgtcccct gggttcgtct cgcccaactc
     3361 gttcccgtcc gctttgcgct ggataaagtt ccaggcgatg tcacgctggt gtcgggcacc
     3421 acctgtagca ttgccgtagg tcaataatga acgcatcgtc atggtccttg cgcaatttgc
     3481 cctggttcag ggccacgctg gcgcaatggc gttatgcgtt acgcaatacc attgccatgt
     3541 gtctggcgct gacggttgcc tattatttga atctggatga accctattgg gcgatgacct
     3601 cggctgcagt ggttagcttt cccaccgttg gcggtgtcat cagcaaaagc ctcggacgca
     3661 tggctggcag tttactggga gccattgcgg cactgcttct tgccggacat acgctcaatg
     3721 agccgtggtt ttttctattg agcatgtcgg cgtggcttgg cttttgtacc taggcctgtg
     3781 cgcacttcac gaataacgtc gcgtatgcat ttcaactggc gggctacacg gctgccatca
     3841 tcgcctttcc gatggttaat attactgagg ccagccagct gtgggatatc gctcaggcgc
     3901 gcgtttgcga ggtgatagtc ggtattttgt gcggcggcat gatgatgatg atcctgccta
     3961 gcagttccga tgctactgcc cttttaactg cattgaaaaa catgcacgcc cgattactgg
     4021 aacatgccag tttactctgg cagcctgaaa caaacgatgc cattcgtgca gcacatgagg
     4081 gggtgattgg gcagatactg accatgaatt tgctgcgtat ccaggctttc tggagccact
     4141 atcgttttcg ccagcaaaac gcgcgcctta atgcgctact ccaccagcaa ttacgtatga
     4201 ccagtgtcat ctccagcctg cgacgtatgt tgctcaactg gccctcaccg ccaggtgcca
     4261 cgcgagaaat tctcgaacaa ttgctgacgg cgctcgccag ttcgcaaaca gatgcttaca
     4321 ccgtcgcacg tattatcgcc ccgctacgcc cgaccaacgt cgccgactac cggcacgtcg
     4381 ccttctggca gcgactacgt tatttttgcc gcctttatct gcaaagtagt caggaattac
     4441 atcgtctgca aagcggtgta gatgatcatg ccagactcgc acggacatcc gctctggctc
     4501 gtcataccga caacgccgaa gctatgtgga gcgggctgcg tacattttgt acgttgatga
     4561 tgattggcgc atggagtatt gcatcgcaat gggatgccgg tgccaatgca ttaacgctgg
     4621 cagcaattag ctgcgtactc tactccgccg tcgcagcacc gtttaagtcg ttgtcactgc
     4681 tgatgcgcac gctggtgtta ctttcgctat tcagctttgt ggtcaaattt ggcctgatgg
     4741 tccagattag cgatctgtgg caatttttac tgtttctctt tccactgctg gcgacaatgc
     4801 agcttcttaa attgcagatg cccaaatttg ccgcactgtg ggggcaactg attgttttta
     4861 tgggttcgtt tatcgctgtc actaatcccc cggtgtatga ttttgctgat tttcttaact
     4921 ataatctggc aaaaatcgtt ggcgttgcgc tggcttggct agcgttcgcc attctgcgtc
     4981 caggatcgga tgctcgtaaa agccgccgcc atattcgcgc gctgcgccgg gattttgtcg
     5041 atcagctaag ccgccatcca acactgagtg aaagcgaatt tgaatcgctc acttatcatc
     5101 acgtcagtca gttgagtaac agccaggatg cgctggctcg ccgttggtta ttacgctggg
     5161 gtgtagtgct gctgaactgt tctcatgttg tctggcaatt gcgcgactgg gaatcgcgtt
     5221 ccgatccgtt atcgcgagta cgggataact gtatttcact gttgcgggga gtgatgagtg
     5281 agcgtggcgt tcagcaaaaa tcactggcgg ccacacttga agaattacag cggatttgcg
     5341 acagccttgc ccgtcatcat caacctgccg cccgtgagct ggcggcaatt gtctggcggc
     5401 tgtactgctc gctttcgcaa cttgagcaag caccaccgca aggtacgctg gcctcgtaat
     5461 tacttaatta caccacaggc atagcgttca ccgccaccgc ccagcggttt aggttgatcg
     5521 gacatattat cgccgccaac gtggaccatc agcgccttgt ctttgatttc atccagtgat
     5581 ttcagacgag gcgcgatgac ggcatcggta gctttgccgt cattattgac gaccagtgca
     5641 ggcagatcgc ctaaatgccc ggtaccttct ggcccttcat gtttaccggt attttgtgga
     5701 tcaagatgcc cgcctgcgga ttccgcggcg ctggctttgc catctttggt ggctggctgg
     5761 cagcttcctt tggcatgaat atggaagcca tgttcaccgg ggggtaatgc tttcagatcg
     5821 ggtgaaaact ccagaccttt atcggtttca gtaatggtga cgctaccaat tgactgccca
     5881 accccttgcg acgtgacgag gttcatctcg actttttcac tggcagcttg tgcgccggtc
     5941 gcaacaacca gcgccagaat ggccagacta aaacgtttca taggacctcc gttcattgtg
     6001 cattcctgaa taagtgtaaa ccagtgacac atttttgaac ggcggctatt cctaaaagtg
     6061 cttacggtac gtcgtacccc agtgcagctt tacggatacg aaaccattgt tgacgggtca
     6121 ttttcagtgt ttctgcttcg acagctgccc gtacgcgctc aattttacct gagccgataa
     6181 ttggcagcgg ctgcgacggt aaacgtaata cccaggcgta aaccacctgt tcaatcgagc
     6241 ccgcgtttaa ctcctccgcc accacagcca gttcatcacg cagcggctgg aaataatcat
     6301 cattaaacag acgaccacca ccaaggcagg accacgccat cggacgaaca cgcagttgtt
     6361 gtagttggtc gagcgtgcca tccagcagta acggctgatg caccggggat atttccacct
     6421 gattagtggc aagggtaaac ggcagacgtg attgcaacag ggcaaattgc gcaggcgtaa
     6481 agttcgatac gccaaaatga cgcactttgc cgctctgatg cagatgtttg aacgcgtccg
     6541 ccacttcatc ggcatccatt aacgggtctg gtcggtggat taacagcaga tccagatgat
     6601 cggtcgcgag attaattagc gactgttcgg cgctcttaat gatgtgattg cggtcagtga
     6661 tgtaatgacc aatgacgttt tcttcacgcg cggtcgtcgc gataccgcat ttactgacga
     6721 tttccatccg ttcacgcagg tgaggtgcca gtttcagtgc ctcgccaaac gccgcttcgc
     6781 actgatagcc accataaata tcagcatggt ccacggtggt cacgccgaga tccagatgct
     6841 cttcaataaa actgaccagc tggcgggcgg acatattcca gtccatcaat cgccagtagc
     6901 ccatcacaaa acgggaaaac tccgggcctt ccggcgcaat agtaatacgc tgaaccataa
     6961 tcgcttcctc ttatcagata taagaggagt atacgcaaga ttaggttcaa aagagtgatg
     7021 gttgctccgg ttcgtctgat gacgctggct tatttgcgcg taatttgcgc attaatcgct
     7081 gccgacaaag gcgcagcacc tcttgttttt cgccatcgct cattttattc cagttaaaac
     7141 gctcatcccg actacgaaaa cagccgcgac aaaacccgcg ttcgtcagac tggcaaattc
     7201 cccggcacgg gctctggacg ggaaagaact ctaattgctc cgccacctcg ccctcctcag
     7261 ataagattat taccattatt gaagctgtta atgtccaaag tagcaacttt gcttgcacta
     7321 gaccaactgg tctactacac tccaacgcat gaacaaacac accgaacatg atactcgcga
     7381 acatctcttg gcgacgggcg agcaactttg cctgcaacgt ggattcaccg ggatggggct
     7441 aagcgaatta ctaaaaaccg ctgaagtgcc gaaagggtcc ttctatcact actttcgctc
     7501 taaagaagcg tttggcgttg ccatgcttga gcgtcattac gccgcatatc accagcgact
     7561 ggctgagttg ctgcaatccg gcgaaggtaa ctaccgcgac cgcatactgg cttattacca
     7621 gcaaacactg aaccagtttt gccaacatgg aaccatcagt ggttgcctga cagtaaaact
     7681 ctctgccgaa gtgtgcgatc tgtcagaaga tatgcgcagc gcgatggata aaggtgctcg
     7741 cggcgtgatc gccctgctct cgcaggctct ggaaaatggc cgtgagaacc attgtttaac
     7801 cttttgtggc gaaccgctac aacaggcaca agtgctttac gcactatggc ttggcgcgaa
     7861 tctgcaggcc aaaatttcgc gcagtttcga gccactggaa aacgcgctgg cccatgtaaa
     7921 aaacattatt gcgacgcctg ccgtttagca ggcatttttt atcaccagac gaccgggagc
     7981 ctttatgtca tctgaaaaac tgtattcccc actgaaagtg ggcgcgatca cggcggcaaa
     8041 ccgtattttt atggcaccgc tgacgcgtct gcgcagtatt gaaccgggtg acattcctac
     8101 cccgttgatg gcggaatact atcgccaacg tgccagtgcc ggtttgatta ttagtgaagc
     8161 cacgcaaatt tctgcccagg caaaaggata tgcaggtgcg cctggcatcc atagtccgga
     8221 gcaaattgcc gcatggaaaa aaatcaccgc tggcgttcat gctgaaaatg gtcatatggc
     8281 cttgcagctg tggcacaccg gacgcatttc tcacgccagc ctgcaacctg gcggtcaggc
     8341 accggtagcg ccttcagcac ttagcgcggg aacacgtact tctctgcgcg atgaaaatgg
     8401 tcaggcgatc cgcgttgaaa catccatgcc gcgtgcgctt gaactggaag agattccggg
     8461 tatcgtcaat gatttccgtc aggccattgc taacgcgcgt gaagccgctt ttgatctggt
     8521 agagctccac tctgctcacg gttatttgct gcatcagttc ctttcccctt cttcaaacca
     8581 tcgtaccgat cagtacggcg gcagcgtgga aaatcgcgca cgtttggtac tggaagtggt
     8641 cgatgccggg attgaagaat ggggtgccga tcgcattggc attcgcgttt cgccaatcgg
     8701 tactttccag aacacagata acggcccgaa tgaagaagcc gatgcactgt atctgattga
     8761 acaactgggt aaacgcggca ttgcttatct gcatatgtca gaaccagatt gggcgggggg
     8821 tgaaccgtat actgatgcgt tccgcgaaaa agtacgcgcc cgtttccacg gtccgattat
     8881 cggcgcaggt gcatacacag tagaaaaagc tgaaacgctg atcggcaaag ggttaattga
     8941 tgcggtggca tttggtcgtg actggattgc gaacccggat ctggtcgccc gcttgcagct
     9001 caaagctgag cttaacccac agcgtgccga aagtttctac ggtggcggcg cggaaggcta
     9061 taccgattac ccgacgttgt aatccaacat tgcgagcggc gtaaagccgc cgctatacta
     9121 aaacaacatt ttgaatctgt tagccatttt gaggataaaa agatgcgtct tcttcatacc
     9181 atgctgcgcg ttggcgattt gcaacgctcc atcgattttt ataccaaagt gctgggcatg
     9241 aaactgctgc gtaccagcga aaacccggaa tacaaatact cactggcgtt tgttggctac
     9301 ggcccggaaa ccgaagaagc ggtgattgaa ctgacctaca actggggcgt ggataaatac
     9361 gaactcggca ctgcttatgg tcacatcgcg cttagcgtag ataacgccgc tgaagcgtgc
     9421 gaaaaaatcc gtcaaaacgg gggtaacgtg acccgtgaag cgggtccggt aaaaggcggt
     9481 actacggtta tcgcgtttgt ggaagatccg gacggttaca aaattgagtt aatcgaagag
     9541 aaagacgccg gtcgcggtct aggcaactaa tctcctgccg ggcgtgaact catcgcgccc
     9601 gcatctttac tgcatcgaca agtaatattt gtcataatgc gcgctgcaat ttatccgtat
     9661 taagagaatc agatgtccga taacgctcaa cttaccggtc tgtgcgaccg ttttcgtggt
     9721 ttttatcctg ttgtgatcga tgttgaaaca gccggattta acgccaaaac cgatgcgctg
     9781 cttgagattg ccgccatcac cctgaaaatg gatgaacaag gctggctgat gccggacacc
     9841 acattacatt tccacgtcga accatttgtc ggcgcaaatt tgcaaccaga agccctcgcc
     9901 ttcaacggca ttgacccgaa cgatcccgat cgcggcgcgg tcagcgaata cgaggcgctg
     9961 cacgaaattt ttaaagttgt acgtaaaggt attaaagcga gcggctgtaa ccgcgccatt
    10021 atggtggcgc acaacgccaa ttttgatcac agctttatga tggccgccgc agaacgcgcc
    10081 tcgctgaaac gtaacccgtt ccaccctttc gccacttttg acactgctgc actggccggg
    10141 ctggcactcg gacaaaccgt attgtcaaag gcttgccaga ccgctggcat ggacttcgac
    10201 agcacccagg cgcactccgc gctgtacgac accgaacgca ctgctgtgct gttttgtgaa
    10261 atcgtcaacc gctggaaacg tctgggaggc tggccgctac ctgccgccga agaggtgtaa
    10321 tcgagtcgat gcctgatgac atgcaatgat tcaggcatct atagtgaggc tattccacgc
    10381 atcaagcatg atattcacgg ggaatagcgt taatggcaga taatccagac ccttcatcgc
    10441 tcctgccgga cgtgttttca ccggcgaccc gcgactggtt tcttcgcgcc tttaaacagc
    10501 cgaccgctgt ccagtcgcaa acctggcatg tggcggcgcg aggcgaacat gcgctggtga
    10561 ttgcaccgac tggctccggg aaaacgctgg cagcatttct ctacgccctc gatcggctct
    10621 tccgcgaagg cggcgaagat acccgcgagg cgcataagcg taaaacctca cgcatcctct
    10681 atatttcacc gataaaagcc ctgggcaccg acgttcagcg caacttgcag atcccgttga
    10741 agggtattgc cgatgaacgg cggcggcgcg gcgaaacggt aattattgat gaagtccacg
    10801 cggtagcggg cagtaaacgt ggtgcgcatc tggcgttaag tctggagcgg ctcgatgcgc
    10861 tgctccacac ctcagcacag cgaattggcc tttctgccac tgtgcgctca gccagcgatg
    10921 tggcagcatt tcttggtggc gatcgcccgg ttacggtagt caacccgccc acaatgcgcc
    10981 atccgcagat acgaattgtc gtaccggtcg ccaatatgga tgatgtctca tcggtcgcca
    11041 gcggcaccgg cgaagacagc catgccggcc gggaaggctc catctggcca tatattgaaa
    11101 cgggtatcct tgatgaagtg ttgcgccatc gctcgaccat tgtctttact aattcacgcg
    11161 ggctggcgga aaaactgacg gcacgattaa atgagcttta cgccgcacgc ttacagcgtt
    11221 ccccgtctat cgccgttgat gcggcccatt tcgagtcgac ctccggcgca acctctaacc
    11281 gtgtacaaag tagcgacgtt tttattgccc gctcacacca cggctccgtc tctaaagaac
    11341 aacgagcaat caccgaacag gcgctgaaat cgggtgaatt acgctgcgtg gtcgcaacct
    11401 ccagtcttga actggggatt gatatgggcg cggtggatct ggtgattcag gtggcaacgc
    11461 cgctttctgt tgccagtggg ttacaacgca ttggtcgcgc cggacatcag gttggcggtg
    11521 tatctaaagg gctgtttttc ccccgtaccc ggcgtgattt agtcgattcc gcagtcattg
    11581 tagagtgtat gttcgcaggc aggctggaaa acctgacacc accgcataat cctctcgacg
    11641 tccttgcgca gcaaaccgtt gccgccgcgg cgatggatgc attacaggta gacgaatggt
    11701 actcccgcgt acgccgtacc gcaccgtgga aagatctgcc aagacgtgtt tttgacgcca
    11761 cgctggatat gctttccggg cgctatccct ctggcgattt ttctgctttt cgccccaaac
    11821 tggtctggaa cagggagacc gggatattga ccgcccgacc tggcgctcaa ttgttggcgg
    11881 ttaccagcgg cggcaccatt ccggatcgtg gcatgtatag cgtgttatta cccgaaggtg
    11941 aagaaaaggc cggttcgcgg cgggtgggtg aactggatga ggagatggta tatgagtcgc
    12001 gggtgaacga cattatcact ctcggcgcta cctcatggcg gatccagcaa atcacccgcg
    12061 atcaggtgat tgtgactcct gctccgggtc gttctgccca gctccccttc tggcgtggtg
    12121 aaggtaacgg acgtccggct gaattaggcg agatgatcgg cgattttctt catttactgg
    12181 cggatggcgc gttcttttcc gggactattc ccccgtggct ggcagaagaa aatacgaacg
    12241 ccaatattca ggggttgatt gacgagcaac gcaacgcgac gggcatcgtt ccggggagtc
    12301 gccatctggt cctcgaacgg tgccgtgatg aaattggcga ctggcgtatt attttgcact
    12361 ctccctatgg aagacgggtg catgaaccct gggcgctggc gattgccggg cgaatacatg
    12421 ccctatgggg cgctgacgcg tctgtggtcg ccagtgatga cagcattgtt gcgcgcattc
    12481 ctgacactga tggtaaattg cccgacgccg cgattttttt gttttaacca gaaaagttgc
    12541 tgcaaattgt ccgcgaggcg gtaggcagct cggcactttt cgccgcccgt tttcgcgaat
    12601 gcgccgcgcg ggcattatta atgccgggcc gcacgccggg ccatcgcacc ccgctttggc
    12661 aacaacggct gcgcgccagt cagttgctgg aaatcgctca gggatatccg gattttccgg
    12721 tcattctcga aaccctacgc gaatgtctgc aagatgttta tgatcttccc gcactggaac
    12781 gtttgatgcg tcgcctgaac ggtggcgaaa ttcaaatatc cgatgtaacg accactacgc
    12841 cctcgccttt cgccacaagt ttattgttcg gctatgtcgc ggaatttatg taccagagcg
    12901 acgccccgct ggcagagcgc cgggcatccg tactgtcgct ggacagcgag ttactgcgca
    12961 atctactcgg acaggtcgat ccgggggaat tactcgaccc gcaggtcatt cgccaggtgg
    13021 aagaagagtt gcaacgactg gctcctggta gaagagcgaa aggtgaagaa ggattgttcg
    13081 acctgctgcg cgaactgggg ccaatgaccg ttgaagacct ggcgcaacgg catacaggca
    13141 gcagtgaaga ggttgcgtcg tatctggaaa atcttcttgc agtaaaacga atcttcccag
    13201 cgatgattag cggacaggag cgtcttgcct gtatggatga tgccgccagg ctgcgtgatg
    13261 ccctcggcgt acggctacca gagtcattgc cagagattta tttacataga gtcagttacc
    13321 cgcttcgcga cctctttctg cgctatctcc gggctcatgc tctggtcacg gctgaacaac
    13381 tggctcatga gtttagtctc ggtattgcca ttgtcgaaga gcagcttcag caactgcgtg
    13441 aacagggtct ggtgatgaat ctgcaacaag acatctgggt gagcgatgaa gtatttcgtc
    13501 gtctgcgttt gcgctcgctg caagccgcca gagaagcgac gcgtcccgtt gcagccacga
    13561 cctatgcgcg attgctgctg gaacgtcagg gcgtattacc cgccaccgat ggtagcccgg
    13621 cgctctttgc ctcaacatct ccaggcgttt atgagggcgt agatggagtg atgcgggtga
    13681 tcgaacagct tgccggagtc ggtttacccg cctcactctg ggaaagccag atcctgcctg
    13741 cccgcgtacg cgactattcg ccagaaatgc tcgatgaatt actggcaacc ggagcggtta
    13801 tctggtcggg acaaaaaaaa gctgggtgaa gatgacggcc tggtggcact gcatctacag
    13861 aaatatgccg cagaatcgtt cactcccgcc gaagcggatc aggcgaatcg ttcggcgctg
    13921 cagcaagcga taatcgctgt tctggctgac ggaggagcct ggtttgcaca acaaatcagc
    13981 caacggatac gcgataaaat cgtcgaatcg gttgattcct cggcactgca agaggcgtta
    14041 tgggcgctgg tctggcaagg cgtcattacc agcgatattt gggcaccgtt acgcgccctc
    14101 acccgcagca gttccaacgc acgcacctca actcgccgca gtcaccgggc tcgtcgtggg
    14161 cgtcctgtct atgcacaacc cgtctcgccg cgggtatctt acaacacacc aaatctggct
    14221 ggacgctggt cgttattgca ggtggagcca ctaaacgata ccgaaaggat gctggcgctg
    14281 gcggaaaata tgctcgaccg ctacggcatc atcagtcgtc aggcggtgat agccgaaaat
    14341 atccctggcg ggtttccatc gatgcaaaca ctttgtcgaa gtatggaaga ctccgggcga
    14401 attatgcgag gtcgttttgt agaaggtctg ggtggcgcgc aattcgctga acgtctgact
    14461 attgaccgat tgcgcgatct ggcgacacaa gccacgcaaa cacgccacta tacaccagtg
    14521 gcgctctccg ccaacgatcc ggctaatgtg tggggaaatc ttctgccctg ggctgcacat
    14581 ccggcaacgc tggtaccaac gcgtcgggcg ggtgcgctgg tggtcgtttc tggcggcaaa
    14641 ttgttactct atctggcgca aggcggcaaa aaaatgctgg tctggcagga aaaggaggaa
    14701 ttactcgccc cagagatttt ccaggcgctg accaccgcac tgcgtcgcga accaaggctg
    14761 cgctttacgc taacagaagt gaatgatctt ccggtccggc aaacgccgat gtttacgctg
    14821 ctgcgtgagg cgggattttc aagttcgcca caagggctgg attggggata gttgacatcc
    14881 tccacgccct gaaagacgtg gttccctgct acgttcaagc tgtcgcctga atcatttcgg
    14941 tgggttcctg cttcaacgag cggcctgact gcaccatctc tccacaggca agaacggcgt
    15001 gccccgccgc taaaatgtta cgagcgccat ttacatcggc gttcgctgta tatccacata
    15061 cctggcatct gaatttactt tgtgacaggc ggttttcttt cgctgtatga ccacagcacg
    15121 cgcaacgctg gcttgtgtac gctggcggaa cagcaagcac ctgaccgcca ctccagagct
    15181 gcttgtactc aagctggcgg cgcatttcat accagccctg atccagtatc gaacggttta
    15241 aacctgattt tgcccggaca ttgcgccccg gctggctgac cgtacccgct gctgactttg
    15301 acatgttgct gaccttcaaa tcctcaatga ctatcattgc gtggtttttg ctgacggtcg
    15361 ttgtgacttt gtgaaggtag tccctgcgga tatttgcgat acaggaatgc agtcgctgta
    15421 ttttgcgttt ctgcttctgc cagttgttgc tgaatttgac cttccggctt aactgtcgct
    15481 gaagtctcgc cagcgtcttc tggttttttt gaaaactgtt tacaggctca aagactgtgc
    15541 catctgacag cgtggcgagt ttagccacgc cagcatccag cccgatcatt gatgctgaag
    15601 ggtgaaccgg agttgatact tcactttctg tctgaatact gatgtaccac gtaccgcagg
    15661 actggctgac agtgacattt ttcacaactc ccgtgacctg acggctattc cggtagcgca
    15721 tccagccaag tttcggcaga aaaatacggc tgttttcctg atcgagctta acgtcctgcg
    15781 ggtagcggaa tgcagcattc tgtccccgct ttttgaatcg gggaaaagca gcccgcttct
    15841 ggaagaagtt tttgtaagcc cgctcaaggt ctttcagtga ctgttgcaat ggctgtgagg
    15901 gcgaatcttt aagccattgc gtttcagtgg cgtttttcca ctcaaccagc caggaagcca
    15961 ttttcccgta agggatgtat ttattaccgg cctcatgatt ctcattctga agcgccagag
    16021 cacgattgaa aacgaaacga catgcgcctg cgaagcgcct catttcacgc tcctgttgac
    16081 cacc
//
LOCUS       DAJTQQ010000086        15901 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000086 DAJTQQ010000000
VERSION     DAJTQQ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15901
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.086"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            446..1504
                     /gene="citC"
                     /locus_tag="OO309_003055"
     CDS             446..1504
                     /gene="citC"
                     /locus_tag="OO309_003055"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR5848115.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1519..1815
                     /gene="citD"
                     /locus_tag="OO309_003056"
     CDS             1519..1815
                     /gene="citD"
                     /locus_tag="OO309_003056"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR5848116.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1812..2720
                     /gene="citE"
                     /locus_tag="OO309_003057"
     CDS             1812..2720
                     /gene="citE"
                     /locus_tag="OO309_003057"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR5848117.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2731..4263
                     /gene="citF"
                     /locus_tag="OO309_003058"
     CDS             2731..4263
                     /gene="citF"
                     /locus_tag="OO309_003058"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR5848118.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4267..4817
                     /gene="citX"
                     /locus_tag="OO309_003059"
                     /pseudo
     CDS             4267..4817
                     /gene="citX"
                     /locus_tag="OO309_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4792..5670
                     /gene="citG"
                     /locus_tag="OO309_003060"
     CDS             4792..5670
                     /gene="citG"
                     /locus_tag="OO309_003060"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR5848119.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5721..7183
                     /gene="citT"
                     /locus_tag="OO309_003061"
                     /pseudo
     CDS             5721..7183
                     /gene="citT"
                     /locus_tag="OO309_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7297..8103
                     /gene="rna"
                     /locus_tag="OO309_003062"
     CDS             7297..8103
                     /gene="rna"
                     /locus_tag="OO309_003062"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR5848120.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8332..8742
                     /gene="rnk"
                     /locus_tag="OO309_003063"
     CDS             8332..8742
                     /gene="rnk"
                     /locus_tag="OO309_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR5848121.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8973..10182)
                     /locus_tag="OO309_003064"
                     /pseudo
     CDS             complement(8973..10182)
                     /locus_tag="OO309_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
     gene            10403..10831
                     /gene="uspG"
                     /locus_tag="OO309_003065"
     CDS             10403..10831
                     /gene="uspG"
                     /locus_tag="OO309_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR5848122.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10952..12517)
                     /gene="ahpF"
                     /locus_tag="OO309_003066"
     CDS             complement(10952..12517)
                     /gene="ahpF"
                     /locus_tag="OO309_003066"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR5848123.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12762..13325)
                     /gene="ahpC"
                     /locus_tag="OO309_003067"
     CDS             complement(12762..13325)
                     /gene="ahpC"
                     /locus_tag="OO309_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR5848124.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13697..14443
                     /gene="dsbG"
                     /locus_tag="OO309_003068"
     CDS             13697..14443
                     /gene="dsbG"
                     /locus_tag="OO309_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR5848125.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14653..15555
                     /locus_tag="OO309_003069"
     CDS             14653..15555
                     /locus_tag="OO309_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5848126.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15702..15901
                     /locus_tag="OO309_003070"
                     /pseudo
     CDS             15702..15901
                     /locus_tag="OO309_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         3948 a         4105 c         3847 g         4001 t
ORIGIN      
        1 gatacgcagc ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg
       61 caacattgtt ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg
      121 aatcaaaata catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa
      181 atcacttaaa cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa
      241 ataaataaaa aaccactaac tctatgtgaa ataaatcaaa atttcacgcc gaaatactcc
      301 ttaggatgta tagcaaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt
      361 gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac
      421 atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg
      481 ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt
      541 tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg
      601 tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt gaatccgttc gcggtgaagg
      661 actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca
      721 tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac
      781 gctgacctgc gtacccggcg tgatggtgct gatggaaaac agcgccacgc gactgaaacg
      841 ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct gcattgtgat
      901 gaacgccaat ccctttacga atggtcaccg ttatctgatt caacaagctg cggcacagtg
      961 cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg
     1021 gctggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc
     1081 agaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac agagcgtcat
     1141 taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg ctcccgcgct
     1201 tggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa
     1261 ccaggatatg cgctactggc tggaaacgcc gactatctcc gcagcgccca tcgaactggt
     1321 tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact
     1381 gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta
     1441 tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agacccccgc
     1501 atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct
     1561 ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat
     1621 agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc
     1681 cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact ggactgcatt
     1741 ttacatgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg
     1801 gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca
     1861 tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg
     1921 atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac accgcccgcc
     1981 gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg
     2041 tcaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt cgcggtggtg
     2101 cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat attgaaaaag
     2161 agatcctgcg tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg
     2221 cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct tccgagcgtt
     2281 tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc
     2341 cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg
     2401 gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag
     2461 aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga
     2521 ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccaccgcg
     2581 tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca
     2641 agatggtgga cggtccggtt atcgatcgcg cccgtctggt gctctcccgt gcagaacttt
     2701 ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga
     2761 caagaacggg tagcggcctg gaatcgtcgc actgaatgcg atcttgccgc tttccagaac
     2821 tcaccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg
     2881 gaagaagcga ttcgtcgctc aggtttacag gacggcatga cggtttcctt ccatcacgct
     2941 ttccgtggcg gtgacctgac cgtcaatatg gtgatggacg tcatcgcgaa gatgggcttt
     3001 aaaaacctga ccctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac
     3061 attcgccagg gcgtggttac ccgcatttat acctctggcc tacgcggtcc actggcggaa
     3121 gagatctccc gtggtctgct ggcggaaccg gtgcagatcc actctcacgg cggtcgtgtg
     3181 catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgt
     3241 gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat
     3301 gcaatggttg atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga actgctgcct
     3361 tatccgcata atccggcaag cattgagcaa gatcaggttg atttgatcgt caaagttaat
     3421 cgcgttggcg atgctgcaaa aatcggcgct ggtactaccc gtatgaccac taacccgcgc
     3481 gaactgctta ttgcccgtag cgctgcggat gtgattgtca actctggcta cttcaaagaa
     3541 ggtttctcca tgcaaaccgg caccggcggc gcttcgctgg cggtaacccg tttcctggaa
     3601 gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggcggtat taccgcgacg
     3661 atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca gagctttgac
     3721 agccatgctg cgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag
     3781 tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt ggtactgagc
     3841 gcgctggaaa ttgacaggca gttcaacgtt aacgtgctga ccggttctga cggcgtactg
     3901 cgtggcgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc
     3961 gcgccactgg tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc
     4021 ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt
     4081 ccggaactag cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg
     4141 cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc
     4201 gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa
     4261 taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg
     4321 ctcgtcagcc gggatgaaag gcaagcacgg caacacgcct ggctcaagca ccatcctgtt
     4381 ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagcga ggtcacacgc
     4441 cgaattttta atcatcgcgt gacagccttg cgtgccttag ccacaaagca gggctggcaa
     4501 attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt gagcattgcc
     4561 gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca tcctctcggg
     4621 cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg ccgcgactat
     4681 tcactgcgcc tcgccgctgc ctgttgtgcg agcaaagcgc agccgtctgc gcgcgtggaa
     4741 aaacccatca actgaccgat ttactcaacc gcatggaggc actgctgaac gatgtcgatg
     4801 cctgcaacgt caactaaaac cacaaagctt gcgacgtcat taatcgatga gtacgccctg
     4861 ctgggctggc gcgccatgct gactgaagtc aatctgtcac cgaaaccagg cctcgtggat
     4921 cgcattaact gcggtgcgca caaagatatg gcgctggaag atttccaccg cagcgcgctg
     4981 gcgattcagg gctggttacc ccgtttcatt gaatttggtg cctgtagtgc agaaatggca
     5041 ccggaagcgg tactccacgg attacgccca attggtatgg cttgcgaagg tgatatgttc
     5101 cgcgccactg cgggcgtaaa cacgcataaa ggcagcattt tttctttagg gctgctatgt
     5161 gcggcaattg gccgtttgct tcagctcaac cagccggtta cgccaacaac cgtttgttct
     5221 acggcggcaa gtttctgccg tggcctgacc gatcgcgaac tgcgtaccaa taattcacga
     5281 ctgacggcag gtcaacggtt gtaccagcag cttggcctta ccggcgcacg cggtgaagcc
     5341 gaagcaggtt atccactggt gatcaaccac gccttgccgc attacctcac tctgctggat
     5401 caggggttag atcctgaact ggcattgctc gataccttgc tcctactgat ggctaccaac
     5461 ggcgatacca acgttgcatc gcgcggtggc gaggggggcc tgcgctggct acagcgcgag
     5521 gcgcaaacat tattgcaaaa agggggcatt cgaaccccca ccgatctcga ttatctccgg
     5581 cagttcgaca gggagtgtat cgaacgaaat ctcagtccag gcggcagtgc cgacctgctg
     5641 atccttacct gttttttagc acagatttaa ttatttaagc acttgataaa tttggaaata
     5701 ttaattttcg gagaacccgt atgtctttag caaaagataa tatatggaaa ctattggccc
     5761 cactggtggt gatgggtgtc atgtttctta tccctgtccc cgacggtatg ccgccgcagg
     5821 catggcatta cttcgctgtg tttgtggcaa tgattgtcgg catgatcctc gagccaattc
     5881 cggcaacagc gatcagtttt attgcggtta ctatttgcgt tattggcagt aattacctgc
     5941 tctttgatgc caaagaatta gctgacccag cgtttaatgc gcaaaaacag gcgctgaaat
     6001 ggggcctggc tggtttttcc agcaccacgg tatggctggt atttggcgca tttatttttg
     6061 cattagggta tgaagtttcc gggttaggtc gtcgcattgc ccttttcctg gtgaaattca
     6121 tggacaaacg cacgctgacg ttgggttatg cgattgtcat tatcgacatt ctgctggcac
     6181 cgtttacacc gtccaacacc gcgcgtaccg ggggtacggt ttttccggtc attaaaaacc
     6241 tgccgccgtt gtttaaatca ttcccgaacg atccgtccgc gcgtcgtatt ggcggctatt
     6301 tgatgtggat gatggtcatt agtaccagtc tgagttcgtc catgtttgtc accggtgcgg
     6361 accaaacgtg ctgggtctgg agttcgtcag caaaattgcc ggtatccaga ttagctggtt
     6421 gcagtggttc ctctgcttcc tgccggttgg ggttatcttg cttatcattg cgccgtggct
     6481 ttcctacgtg ctgtacaaac cggaaatcac acacagtgaa gaagtggcaa cctgggcggg
     6541 tgatgaacta aaaaccatgg gtgcgctgac acgcagagag tggacgctga ttggccttgt
     6601 attgctcagc ttaggtttgt gggtatttgg cagtgaagtc attaatgcta ctgcggttgg
     6661 tctgctggca gtttcgctaa tgctggctct gcacgttgtg ccgtggaaag acattacccg
     6721 ctataacagc gcatggaaca cgctggtcaa cctggcaact ctggttgtga tggctaacgg
     6781 cctgactcgt tctggtttta ttgactggtt cgccggtacc atgagtacgc acctggaagg
     6841 attctcacca aacgcaacgg tgattgtact ggttctggtg ttctactttg cacactacct
     6901 gtttgccagc ctgtctgcgc acaccgcaac catgctgccg gttattctgg ccgtcggtaa
     6961 aggtattccg ggcgtaccaa tggaacaact gtgtatcctg ctggtgctgt ctatcggtat
     7021 catgggctgt ctgacgccgt atgcaaccgg tcctggggtg attatttacg gctgtggcta
     7081 tgtgaaatca aaagattact ggcgtcttgg cgcaatcttc ggggtgattt acatctctat
     7141 gttgctgttg gttggctggc cgattctcgc catgtggaac taatacgttc gccaaacgct
     7201 gaataaatca tcacgcccgc caggtaagcc cacctggcgg gcttttttat gatttaatag
     7261 atagtattca gtgtatgtat gagttccaca cccattatga aagcattctg ccgtaacgcc
     7321 gcgttgctcg cggtttctct gcttccctta tcttctgcca acgccgtagc gttgcaggca
     7381 aaacagtatg gcgattttga tcgctatgtc ctggccctct cctggcaaac cggattttgc
     7441 cagagtcaac acgatcgaaa tcgtaacgaa cgagatgaat gtcgcctgca aaccgaaacg
     7501 accaacaaaa ctgattttct gaccgtacat ggtctgtggc caggattgcc taaatcggtt
     7561 gctgcccgtg gtgttgatga acgccgctgg atgcgcttcg gttgcgctac tcgcccaatc
     7621 ccgaatctac cagaagcacg cgccagccga atgtgttcat cgccggaaac cgggttatca
     7681 ctggaaacgg ccgctaaact aagtgaagtc atgccaggag ctggcggacg ttcctgcctg
     7741 gaacgctacg aatatgccaa acacggtgcc tgctttggtt ttgatccgga cgcatacttc
     7801 ggtacgatgg tacgcctgaa tcaagaaatt aaagagagcg aagccggaaa attccttgcg
     7861 gataattacg gtaaaacagt gagccgccgt gactttgacg ccgcctttgc caaaagctgg
     7921 ggaaaagaga acgtgaaagc agttaagcta acgtgccagg gtaaccctgc gtatttgact
     7981 gaaattcaga tctcgatcaa agctgacgcc atcaacgctc cgctttctgc aaactcattc
     8041 ttgccacaac ctcacccagg taactgtggc aaaacctttg tgattgataa agcgggttat
     8101 taatcctctg atttacaaaa cacgctaatt cgtcttccat aaccatgccg attaagaatt
     8161 atacataatg cgctgcattg aggaattccc tgaatgtgac gcaaatcact tcaaagagtg
     8221 agcaacagtc agtatcatca tgtgagttaa accctcgccg cctgacggtg agggttttct
     8281 tttgggtatg tttatctgcg acactcgcag taccgacgac atggagtaaa aatgtccaga
     8341 ccaactatca tcattaacga cctggatgcc gaacgcatcg atattctgct ggagcaaccc
     8401 gcctatgctg gtttgccaat cgccgacgcg ttaaacgcag agttggatcg cgcccaaatg
     8461 tgttcgccag aagagatgcc acacgacgtg gtgacaatga acagccgggt taaattccgc
     8521 aatcttagcg atggcgaagt gcgtgtgcgc acgctggtgt atccggcaaa aatgaccgat
     8581 agcaatactc agctttccgt tatggctccg gtaggtgccg cactgctggg gctgcgcgtt
     8641 ggcgattcca ttcactggga acttccgggc ggcgttgcaa cccaccttga agtgctggaa
     8701 ctcgaatacc agccagaagc tgctggcgac tacctgcttt aatcccatct gcacaagcga
     8761 taactcgctt tgcccagagg atgcactcgc atcctcttta cgcaaaatca gcaatttttc
     8821 ctggttctca ctgttcactt ttgctgcaaa attattccgt ttcgtttgtg ggaataacca
     8881 tggcagaagc attttacatc ctgataggat ttctgattat ggcggcgatt atcgtcatgg
     8941 ctgtccttta cctcgaaaac cactcctgac gatcatattg ttccccccgg catcgcattc
     9001 accagacctg aaaccgcctt ctgcgccgcc tctgcgcttt gtgcaccggg taccagaatc
     9061 accttacggc actcctcttc acgtttttcg aaaatctcat atccccgggc ggcctcttca
     9121 aacggcatat agtgggtaac aatttcttct ggtttcagta atcctttctc aattaacggt
     9181 aataattctc ccagccatgc gtgaacgtgg gtctgtccca ttttaaacgt caacccttta
     9241 tcaaaggcgt cgccaaacag gaaaccgtga ataaatccag cgtagacgcc cggtacgcta
     9301 acaatgccgc aacgctttac cgctgctgcc ctccagtttc aggttagtca gcaccgtttc
     9361 cgtggtgctg cctttcgctt caaaaccgac ggcgtctatt actgcatcca cgccccggtg
     9421 acctgccgtt tgttcaataa ttgactgtgc cggatcgcta tcttcatcga aattaatcgg
     9481 gatcgcgccg tagcggtcag cggcgaaacg caagcggtag ggatgatgat caacaacaaa
     9541 aatctgttcc gcaccgagca accgtgcaca ggcgattgtc aacaatccca caggaccagc
     9601 accatagact gcaacgcttg aaccttgttg gatctgcaca ttttttgctg cctgccatgc
     9661 cgttggcaga atatcagaaa ggaaaagggc tttatcatct gaaagcaaag gcggtacttt
     9721 aaacggcccc acattccctt tcgggacgcg gacatattcc gcctgcccgc caggaacgcc
     9781 accatacagg tgactataac caaacaatgc cgcgggagca ggtatctgtt ttttattgag
     9841 cgcagcgcct ttacccgcat tggtattttc gcaggcggca tattgctgta atcgacagaa
     9901 aaaacagtcg ccacaagcaa tgacgaacgg aattaccacc cggtcgcctt tttgcaaatt
     9961 ttttacgtcc ttcccggttt caacgacttc ccccataaat tcatgaccaa aaatatcgcc
    10021 atgtttaacc tggggtattt tgcctcgata aagatggagg tcagagccac agatcgccgt
    10081 taccgtaata cgcagaataa tatcatctgc ctgttcaatg cccggatcgg gaacattttc
    10141 tacctgaacg tgatgtgggc cgtgataagt caatgctttc atgcaacctc cagagacagt
    10201 tcaaaaaaga gtcagtcttt aagggtagct atcacagcgg gaaaacgggc ggtaccgcag
    10261 gttttgggag aattcctagt gttcagtgag aatatattaa tcacagaaac cgttcgtaag
    10321 gaaaaagaac cagacaaata tcagggataa atattataat catggagaat gatttgttca
    10381 tgattaacag ggagaaaggg ttatgtataa ggcaatcatt atgccagttg atgtatttga
    10441 aatggaattg agcgacaaag ctgttcgcca cgctgaattc ctcgcccagg atgacggagt
    10501 tattcatcta cttcacgtac tacccgggtc agccagcctg agcctgcacc gttttgccgc
    10561 tgatgtgcgt cgttttgaag agcatctgca acatgaagca gaagaacgtc tgcaaacgat
    10621 ggtcagccac ttcaccatcg atccttcccg cattaaacaa catgtccgtt ttggtagcgt
    10681 gcgggatgaa gtcaatgagt tggcagaaga actgggggct gatgttgtag ttatcggttc
    10741 tcgcaaccca tcgatttcga cccatctgtt aggttctaac gcctcgagcg tgatccgcca
    10801 cgccaatctg cctgtactgg tcgttcgcta acagataaaa gtatttatgc cgccctgcct
    10861 ggaacatccc ggcagggctt tttaatgcca taaaaaagcc gccaggtttg accccggcgg
    10921 ctaagcaatt gcaggtgaat cttacttctt cttatgcagt tttggtgcga atcaggtagt
    10981 caaaagcact cagagaggct ttggcacctt cgccagtggc gatgatgatc tgcttgtacg
    11041 gaaccgtcgt acagtcaccc gctgcgaaca cgcctttgac gttggtttca catttcgcat
    11101 caatgataat ctcgcccatg cggttacgtt cgactgcgcc ttccagccag ttggtgttcg
    11161 gcagcagacc aatctggaca aaaataccgg ccagttcgat gttgtgaata ttgccgctga
    11221 cacgatctcg atattccaga ccaacgactt tgctgccgtc gcctttcact tccgtggttt
    11281 gcgcattcag aataatgtcg acgtttttca ggctgcgcag tttgtcctgc agaacctggt
    11341 cggctttcat ttctggcgca aattccagca gcgttacgtg ctcaacgata cccgccaggt
    11401 cgattgccgc ttccacgccg gagttaccgc cgccgataac cgctacgcgt ttgcctttaa
    11461 acagcgggcc gtcgcagtgc gggcagtagg tcacgccttt agtgcgatac tgatcttcgc
    11521 ccggaacgtt catgttgcgc cattttgcac ccgtcgccac gataatgctg cgtgctttca
    11581 gtaccgcgcc agaagctgtt tcaatctggt gtagaccacc ttcaaccgct gccgggatca
    11641 gtttgctggc gctctggctg tcgatcacat caacgtcgta ttcatcaacg tgaactttca
    11701 gtgcgcctgc caacttctgc ccttcagtct tcggtacaga aatgtagttt tcgatatcaa
    11761 cggtatcgag gatctgacca ccaaaacgtt cgcccatcag accggtacgg atgcctttac
    11821 gtgcggagta aattgctgcc gctgcacccg ccgggccgga accaacgatt aatacgtcat
    11881 aagcatcacg cttgttcagc tcttctgccg cacgattttc cgcgccagta tcaattttgg
    11941 caacgatttc agtcaacgtc atgcggccct gaccaaactc tttcccgttt acgaacactg
    12001 ccggaacgcc catcacgttg cgatcggtga tttcgttctg gaaggtgccg ccgtcaattg
    12061 cagtgtgctt gatgcgcggg ttcaatacgc tcatcaggtt cagcgcctgc accacgtccg
    12121 ggcagttatg gcaagagagc gagtaatagg tttcgaattc aaaatcaccg tcaatatggc
    12181 gaatctgctc cagcagagac tgcgcttctt tcgacggatg accaccggtc cacagcaacg
    12241 ccagtaccag cgaggtgaac tcgtggccca gcggggagcc tgcaaaacgt ggtccctggt
    12301 tggaacctgg gttggtgatc aggaaagacg gcttacgcac tggcaagctg ttatcttctt
    12361 taaaggtgac tttgtctgac agttctgcga tttcagccaa cagttccttg atttctgccg
    12421 atttagcgct gtcatccagc gtggcaatta actcaacagg cttggtcaat ttctcaaggt
    12481 aagccttgag ttgagttttc atatttgtgt cgagcatgtt tatctcctgg gctttaaaca
    12541 tcatcatgca agcggcctct gcggctacct ggatgcagct tgcatcatgg tgcggggaag
    12601 aaaatcgggt gccgcagcac ccgaagaatt aggtgaattt ccgaagtatt tcgcgtcaga
    12661 gcaaggcgac tagcctgtga atccccggga gcttacacaa gtaagtgacc ggggtgagcg
    12721 ggcgacgcca acgccgctat ggcgtgaaag acgaaggaaa tttagatttt accaaccagg
    12781 tccagagacg gagccagagt tgcttcacct tctttccatt tagccgggca aacttcacct
    12841 gggtgagaag ctacgtactg agctgctttg attttacgca gcagatcaga cgcgtcacgg
    12901 ccaatgcctt cagcggtaac ttcgattgcc tggatgatac cctgcgggtc aacaacgaag
    12961 gtcgcacggt cagccagacc ttcatcttca cgcatgttgt cgaagttacg ggtcagggcg
    13021 ccagtcgggt cgccgatcat cgcatatttg attttagcga tggtttcaga gctgctgtgc
    13081 catgctttgt gggtgaagtg agtatcggta gatactgcgt atacgtctac gcccagtttc
    13141 tgcagttctt cgtagtggtc agcaacgtca cccagttcgg tcgggcatac gaaagtaaag
    13201 tcagccgggt agaagaagaa gacgctccag cggccttcgg tatctttttc ggtgatttcg
    13261 atgaattcgc cgtttttgaa tgcctggttt ttaaaaggtt taattttggt gttaatcaag
    13321 gacatctata cttcctccgt gttttcgatg agatgtaagg taaccaattt ttgccgattc
    13381 ggctaatgcg tttccattat caaatcgata agttttacct tacaaccttc gtaagacaac
    13441 tttgtgaact ttgcaccgcc agcggcgata aaaagtaaaa tggccccctt tccaggtgac
    13501 cgtgtaatct gaactaccct ttggtatctt cagcgcctgc ctgcaggctg aggtgttgcc
    13561 cttacaaatg caacaacgac atggattaca acaccctcat aaacaaaggg caatcacctg
    13621 atctaagctc ttacctatga cagtgatagg ttatgccttt tactcgactt ttgcactgac
    13681 tgaaaagaac aaattaatgt taaaaaagat acttttactg gctctgcttc ctgcaatcgc
    13741 cttcgcagag gaacttcctt ccccagtaaa agcgattgaa aaacagggca ttacaatcat
    13801 caaaacgttc gatgcccccg gaggaatgaa aggttatctc ggaaagtatc aggatatggg
    13861 cgtcaccatc tacctgactc ccgatggtaa gcacgctatc tctggttaca tgtacaacga
    13921 gaaaggtgaa aacctgagta acacacttat cgaaaaagaa atttacgcac cagccggacg
    13981 cgaaatgtgg caacggatgg aacaatccca ctggctcctc gacggtaaaa aagatgcgcc
    14041 ggtcattgtc tacgtcttct ccgatccgtt ctgcccatat tgtaaacagt tctggcagca
    14101 ggcgcgcccg tgggtagatt ctggcaaagt gcaattaaga acattgttgg ttggggttat
    14161 caagccagaa agcccggcga cagcagcggc aattcttgcc tccaaagatc ccgcaaaaac
    14221 ctggcaacaa tatgaagcct ctggttgcaa gcttaagcta aacgtgcctg caaacgtaag
    14281 tacagagcaa atgaaagtgt taagtgacaa tgagaaactg atggacgatc tgggggcaaa
    14341 tgtcacgccg gctatctatt acatgagtaa ggaaaatacg ctgcaacagg ccgtggggtt
    14401 gcccgatcag aaaacgctta atatcattat gggaaataaa taaaaggtaa ttcatggatg
    14461 gttgaactat atccctgctt ttcaaccatc ctaaaaaagc atgaatttct cgctctaccc
    14521 taagtggctg tgctatttta gaaaaactta atcctgagtt aagcaaaaaa acataatcaa
    14581 taaaaataca tacgcaaaaa acattgatta agtgaatata ccatggaaga aaaatataaa
    14641 ccggagtagt gtatggccaa tctctacgac ttgaaaaagt tcgacttaaa tcttcttgtc
    14701 atatttgagt gtatttacca gcatttaagc attagcaaag ctgctgaatc actatatatc
    14761 accccatcag ccgtgagcca gtcgttacaa cgcttacgtg cgcaatttaa tgacccttta
    14821 ttcattcgct caggaaaagg tatcgcgccg acgacaacag ggttgaatct gcatcaccat
    14881 cttgagaaaa acctcaaagg ccttgaacaa acaattaata tagtcaataa gtccgaacta
    14941 aagaaaaact ttattatcta cggccctcag ctcatttctt gctctaataa tagtatgctg
    15001 atccgctgcc tacggcaaga tgcctctgtc gagattgagt gccatgatat cctcatgtca
    15061 gcagagaatg ctgaagaact gttggttcac cgcaaagccg atctggtgat cacccaaatg
    15121 ccagtgatca gccgttccgt catttgcatg ccattgcata caattcgcaa tactctaatt
    15181 tgtagtaata agcatccgcg aattactgac aatagcactt atgaacaaat tatggcagaa
    15241 gagttcaccc aattaatagc caaatcagca ggagttgatg atattcaaat ggaaatagac
    15301 gagaagttta tgaatcgcaa aatttcattt cgtggttcct ctttgctgac aataattaat
    15361 agcattgcag ttacagattt actgggaata gtcccctatg aactttataa ctaccaccgt
    15421 gactttctta atttaaaaga gataaaacta gaacatccgt taccttcaat aaagctttac
    15481 atttcctata ataaatcttc tttaaataat ttggtgttct cccgatttat tgaccgtcta
    15541 aatgagagtt tttgatatat aactatagac agctactata actttcatct atttattcac
    15601 agcgcgaatg aaaaataaaa cactctgctt tacagtgttt actttataat taacagtctt
    15661 atctcactgt tcaccctgta acgttcagag agcgtcttca gatgtctatt tataaaattc
    15721 ctcttccgct caatattctg gaagctgcac gagagcgtat tacctagaca ctcaacaccc
    15781 taccccgcgt ttgtgtctca ttttctggcg gcaaagactc tggtttaatg ctgcatctga
    15841 cagccgaact tgcccgacaa atgggcaaaa aaatctgcgt tttgtttatc aactgggagg
    15901 c
//
LOCUS       DAJTQQ010000087        15526 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000087 DAJTQQ010000000
VERSION     DAJTQQ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15526
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.087"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="OO309_003071"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="OO309_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR5848127.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="OO309_003072"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="OO309_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR5848128.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="OO309_003073"
                     /pseudo
     CDS             complement(941..1408)
                     /locus_tag="OO309_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO309_003074"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO309_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR5848129.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="OO309_003075"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="OO309_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR5848130.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="OO309_003076"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="OO309_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR5848131.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="OO309_003077"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="OO309_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR5848132.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="OO309_003078"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="OO309_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR5848133.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="OO309_003079"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="OO309_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR5848134.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="OO309_003080"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="OO309_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR5848135.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="OO309_003081"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="OO309_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR5848136.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="OO309_003082"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="OO309_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR5848137.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="OO309_003083"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="OO309_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR5848138.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO309_003084"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO309_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR5848139.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="OO309_003085"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="OO309_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR5848140.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="OO309_003086"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="OO309_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR5848141.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="OO309_003087"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="OO309_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR5848142.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="OO309_003088"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="OO309_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR5848143.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="OO309_003089"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="OO309_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR5848144.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="OO309_003090"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="OO309_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR5848145.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="OO309_003091"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="OO309_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR5848146.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="OO309_003092"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="OO309_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR5848147.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="OO309_003093"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="OO309_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR5848148.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="OO309_003094"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="OO309_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR5848149.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="OO309_003095"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="OO309_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR5848150.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="OO309_003096"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="OO309_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR5848151.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="OO309_003097"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="OO309_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR5848152.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="OO309_003098"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="OO309_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR5848153.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="OO309_003099"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="OO309_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR5848154.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="OO309_003100"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="OO309_003100"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR5848155.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="OO309_003101"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="OO309_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR5848156.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4007 a         3643 c         4130 g         3746 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgtgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 tgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1021 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggtaagaagg cccatgaatg ctaggcgcca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1261 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagag agcgctgatt tgttaacctg attccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatgc agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcagaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctattgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtaca ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcttgg gtattgtaaa
     5221 accatggaac tctacttggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcatt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggaaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattattgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttacaaa ggcaaggggg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactcta caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcttgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctatctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag atcactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtgtttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cttggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtacgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacttg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtt gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgctt ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca atgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agtatcccca cttatctaca atagctgtac tctttt
//
LOCUS       DAJTQQ010000088        15400 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000088 DAJTQQ010000000
VERSION     DAJTQQ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.088"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..1175)
                     /locus_tag="OO309_003102"
     CDS             complement(<1..1175)
                     /locus_tag="OO309_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5848157.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRL"
     gene            complement(1374..1458)
                     /locus_tag="OO309_003103"
     tRNA            complement(1374..1458)
                     /locus_tag="OO309_003103"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1422..1424),aa:Leu,seq:caa)
     gene            1654..2673
                     /gene="ahr"
                     /locus_tag="OO309_003104"
     CDS             1654..2673
                     /gene="ahr"
                     /locus_tag="OO309_003104"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR5848158.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2802..4304
                     /locus_tag="OO309_003105"
     CDS             2802..4304
                     /locus_tag="OO309_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR5848159.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4423..5505)
                     /gene="lptG"
                     /locus_tag="OO309_003106"
     CDS             complement(4423..5505)
                     /gene="lptG"
                     /locus_tag="OO309_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5848160.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5505..6605)
                     /gene="lptF"
                     /locus_tag="OO309_003107"
     CDS             complement(5505..6605)
                     /gene="lptF"
                     /locus_tag="OO309_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5848161.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6872..8383
                     /gene="pepA"
                     /locus_tag="OO309_003108"
     CDS             6872..8383
                     /gene="pepA"
                     /locus_tag="OO309_003108"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR5848162.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8517..8960
                     /gene="holC"
                     /locus_tag="OO309_003109"
     CDS             8517..8960
                     /gene="holC"
                     /locus_tag="OO309_003109"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR5848163.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8960..11815
                     /gene="valS"
                     /locus_tag="OO309_003110"
     CDS             8960..11815
                     /gene="valS"
                     /locus_tag="OO309_003110"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR5848164.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11869..13065)
                     /locus_tag="OO309_003111"
     CDS             complement(11869..13065)
                     /locus_tag="OO309_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCR5848165.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13258..13761
                     /locus_tag="OO309_003112"
     CDS             13258..13761
                     /locus_tag="OO309_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5848166.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13807..14223)
                     /gene="rraB"
                     /locus_tag="OO309_003113"
     CDS             complement(13807..14223)
                     /gene="rraB"
                     /locus_tag="OO309_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR5848167.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14385..15398
                     /gene="argF"
                     /locus_tag="OO309_003114"
     CDS             14385..15398
                     /gene="argF"
                     /locus_tag="OO309_003114"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR5848168.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3973 a         3930 c         3968 g         3529 t
ORIGIN      
        1 atcaacctac gctccccaag atgttccgcc ttatggatgt aagctgctcg cacagagcta
       61 cgctcctggt gactcatctg ccgctctacc gcatccctcg accataatcc cgactcaatc
      121 aacgaactac aagccatcgt cctgaagcca tgcccacaaa cctcgacctt cgtgtcatac
      181 cccattacgc gcaaagcctt gttcaccgta ttctcactca tcggcttacg tggatcgtga
      241 tcgcccacaa aaatcagctc tcgatttccg ctcatgcttt tgatcttttc caaaatactt
      301 agggcctgcc gcgacaaagg tacaaggtgt ggtgtccgca tcttcgagcc acgctgagaa
      361 tgcttgactc cttccagttg ctcacgctca cctgggattg tccacatagc cgtttcaaaa
      421 tctatttctg accagcgggc aaaacgcagc tcgctcgaac ggatgaaaac caataaagtg
      481 agttcaaccg ccagtcgagt taacggtctg ccagaatagt gatctatgcg atgaagtaat
      541 tcaggtatac ggttcagttc aagagctgca cggtgctgtc ttttcgccgt agcaaccgca
      601 cctgcaatct cttgcgcggg gttgtagtcg attaagccgc tctgaacggc aaagcgcata
      661 atcgcggtag tacgctgctg taaacgggcg gcaacttcga gccgcccgga tgactcgact
      721 gctttaatgg gtacaagaag atcccgcgtc ttcagttccg caatgttccg cttaccgata
      781 gcagtaaaga gattatcctc cagacttttc aaaacacgag cgctatgcga tgccgaccac
      841 ttctgattgc tggcgtgcca gtctctggcg actacttcaa acgttatcgc ctcttgttcc
      901 tgttctacct taacagcttt cttgttttca ctgggatcga caccattagc taaaagctta
      961 cgagcctcat cccggcgtgc cctggcatcc gccaacgaca cttcggggta tttccccaat
     1021 gccagcatct tctctttgcc gccaaagcga taacgtagcc gccagtattt ggagccgtta
     1081 gggtgaacca gcaaaaccat gccttcaccg tcggtcagct tataggcttt tgcttcaggc
     1141 ttagccgaac gaaccttcac atcactcaga gccatgatga gtatcctttc aagggttctg
     1201 tgtgggtaca aacattatcg aaccgggaaa tacccgcagt tgtacccgca tcagtaagtt
     1261 gatgtagatt gaatcaggtt gacttaggtt gagtgaaaaa gcgaggaaag ccttgcggat
     1321 actggatttt aggcacaaaa aaagacgtcc gttgacgtct attgatgttc cgatggtgcc
     1381 gaaggccgga ctcgaaccgg cacgtatttc tacggttgat tttgaatcaa ctgcgtctac
     1441 cgatttcgcc acttcggcac tgaagaggta tgcggaaaac gttgtggatt atacctgtcg
     1501 cacgccacca tgcaagcgcc agcttgcacc cacctcgcta agtgctgaaa ttttcagcat
     1561 tacactcagc gatatgtaac ctttgtcaca ctccaggcac cccgccctgc catgctctac
     1621 acttcccaaa caacaccaga gaaggcccca aaaatgtcga tgataaaaag ctatgccgca
     1681 aaagaagcgg gcggcgaact ggaagtttat gagtacgatc ccggtgagct gaagccacaa
     1741 gatgttgaag tgcaggtgga ttactgcggg atttgtcatt ccgatctgtc gatgatcgat
     1801 aacgaatggg gattttcaca atatccgctg gttgccgggc atgaggtgat tgggcgcgta
     1861 gtggcactcg ggagcgccgc gcaggataaa ggtttgcagg tcggtcagcg tgtcgggatt
     1921 ggctggacgg cgcgtagctg tggtcactgc gacgcctgta ttagcggtaa tcagatcaac
     1981 tgcgagcaag gtgcggtgcc gacaattatg aatcgcggtg gctttgccga gaagttgcgt
     2041 gcggactggc aatgggtgat tccactgcca gaaaatattg atatcgagtc cgccgggccg
     2101 ctgttgtgcg gcggtatcac ggtctttaaa ccactgttga tgcaccatat cactgctacc
     2161 agccgcgttg gggtaattgg tattggcggg ctggggcata tcgctataaa acttctgcac
     2221 gcaatgggat gcgaggtgac ggcctttagt tctaatccgg cgaaagagca ggaagtactg
     2281 gcgatgggtg ccgataaagt ggtgaatagc cgcgatccgc aggcactgaa agccctgtcg
     2341 gggcagtttg atctcattat caatactgtg aacgtcagcc tcgactggca gccttatttt
     2401 gaggcgctga cctatggcgg taatttccat acggtcggtg cggttctcac gccgctgtct
     2461 gttccggcct ttacgttaat tgcgggcgac cgcagcatct ctggttctgc taccggcacg
     2521 ccttatgagc tgcgaaagct gatgcgcttt gccgcccgca gcaaggttgc gccgacaacc
     2581 gaactgttcc cgatgtcgaa aattaacgac gccattcagc atgtacgcga tggtaaagcg
     2641 cgctaccgag tagtcctgaa agccgacttc tgatcccttt gccgggtgct gtcgcccggc
     2701 acttaaatct ctttggaagg cgtctcgcaa tctgctgaat ttagcggtga agagtgcaca
     2761 gccgttgcct atactccagg caggaaactg gaggaaatct catgagcgaa cccctgttaa
     2821 ttgcccgcac gccggacaca gaactgtttt tactgccggg aatggctaac cgtcacgggc
     2881 tgattactgg cgcaacgggg acgggtaaaa ccgttacgct gcaaaaactg gcagagtcat
     2941 tgtcggaaat cggcgtgccg gtgtttatgg ctgatgtgaa aggcgatctg accggtatcg
     3001 cgcaggcagg aacggcgtcg gaaaaactgc tcacaaggct taaaaatatc ggcgtcaatg
     3061 actggcaacc gcatgccaat ccggtggtgg tgtgggatat ctttggcgag aaaggccaca
     3121 cggtgcgggc gacggtttcg gatctggggc cgctgttgct ggcgcgactg ttgaatctca
     3181 acgatgtgca atctggcgtg ctgaatatta tcttccgcat tgcggacgat cagggattgt
     3241 tgctgctcga ctttaaagat ttgcgggcga ttacccagta catcggcgat aacgccaaat
     3301 ccttccagaa tcagtacgga aatatcagta gcgcatcggt tggtgccatc cagcgcggat
     3361 tactgtcgct ggaacagcaa ggcgcagcac acttctttgg tgagccgatg ctggatatca
     3421 aagactggat gcgcaccgat gccaacggta aaggcgttat caatatcctc agcgccgaga
     3481 aactttatca gatgccgaaa ctgtacgccg ccagcctgct gtggatgctt tcagagttgt
     3541 atgaacaatt gccggaagcg ggcgatctgg agaaaccaaa actggtgttt ttcttcgacg
     3601 aggcacatct gctgtttaac gatgcaccgc aggtactgct ggataagatt gagcaggtga
     3661 taaggcttat tcgctcaaaa ggcgtgggcg tctggttcgt ttcgcaaaac ccgtctgata
     3721 ttccggataa tgtgttcggc cagctcggta atcgtgttca gcacgctttg cgggctttta
     3781 cgccaaaaga tcagaaagca gtgaagacag cggcgcaaac catgcgggcc aatccggcat
     3841 ttgataccga aaaggcaatc caggaactgg ggaccggcga ggcgttaatc tcgtttctcg
     3901 atgcaaaagg aagtccttct gtggtggaac gggcgatggt gatcgcacct tgttcgcgaa
     3961 tggggccggt gacggaagat gagcgtaatg gcctgattaa tcactccccg gtgtatggca
     4021 aatatgaaga tgaggtggac cgcgagtccg cctatgagat gctgcaaaaa ggctttcagg
     4081 ccagtaccga gcagcaaaat aatccccccg tgaaagggaa agaggtggcg gtggatgacg
     4141 gtattcttgg tggattgaag gatattttgt ttggcactac cggaccacgc ggcgggaaga
     4201 aagatggtgt ggtgcaaaca atggcgaaaa gcgccgctcg ccaggtgacg aatcagattg
     4261 tacgcgggat gttggggagt ttgctggggg ggagaaaaag ataagtcaga cgtgatctgt
     4321 gcccctcacc ctaaccctct ccccaaaggg gcgaggggac cgttcgaact cgtatttgac
     4381 tttgtcaaca ataataaagg gagctttcgc tcccttgtta gattacgatt ttctcattaa
     4441 cagccacagg ctgattaaga agaagctggc acttggcaac agtgcgccga tgatcggcgg
     4501 gatgccgtaa accaacgtca gcgggccgaa gatctggtcc agtacgtaga agacaaaacc
     4561 gaagctgata cctgtgacca cacgcacgcc catcggtacg ctacgcagtg ggccaaagat
     4621 aaacgacagc gccatcagca tcatcaccgc cacggatagc ggctggaaga ttttgctcca
     4681 catgttgagc tgataacgtc cggcatcctg accgctcgac ttcagatact tcacgtagtt
     4741 gtgcaaacca ctgatggaga gtgcatccgg gtccagcgcc accacgccca gtttgtctgg
     4801 cgtgaggtcg gttttccagg tgccgctcac cgtctgcgaa ccggtaatct gtttcggatt
     4861 ggtcagatca gattcatcaa cctgcgacag acgccagact ttatgttccg ggtcaaactt
     4921 cgcggtagcg gcataacgta cggattgcag acgacgattc tcgttaaagg cataaatact
     4981 gatgccacct aacacctcgt cacctttaac ccgctcaatg tagacgaagt tgttgccatc
     5041 tttcgcccat aaaccttgct gggtagagag caacgagccg ccgtacatcg cctgcgcacg
     5101 gtagttacgc gccatctgtt cgccctgcgg cgcgacccat tcgccaatcg ccatcgtcag
     5161 caagaccagt ggaatggcgg ttttcatcac cgacagcgct acctgcatac gggtaaaacc
     5221 agaagcctgc atcaccacca gttcgctgcg ctgcgccagc atcccaagac caagcaacgc
     5281 cccaagaaga gccgccatcg ggaagaagat ctgcacatct ttcggcacgc tcagcaaggt
     5341 atacattcct gcgcctaacg cgtcgtaact cccctgcccg gcttttttca gctgatcgac
     5401 aaatttgata atgcccgaca gcgacaccag catgaacagc gtcatcatga tggtggtgaa
     5461 aatagtttta ccgatatagc ggtcaagtac gccaaaaggt tgcatcacac cgctccttta
     5521 cgcgaaaaac tggcgcgcag gcggcggacc ggcacggtgt cccaaaggtt gagaacaatc
     5581 gccaaagcca gataaatcag gttaacggtc cacatccaca gtgtcgggtc cagcttacct
     5641 ttaccgccgt tcgatttcag ggaggtctgg atcaggaaga aaagtagata cagcagcatg
     5701 gctggcagca tcgacagtac gcgtccctga cgcgggttaa ccacgctcaa cggtacaacc
     5761 ataagtgcca tcataaacac ggtgaatacc aacgtgatac gccagttcag ttctgcgcga
     5821 gcacgatcgg tgtcagtgtt ccacaatgtg cgcatgtcca tctggtcggt atcgttcggg
     5881 tcgagcgcca ccgcctggtg accaatgatc gcctgataat cctggaaatc cgtaatgcgg
     5941 aaatcacgta acagtgcagt gccttcgaag cgcgttccct ggttgagagt gacgacctgg
     6001 gagccgtcgc gcagctgggt taaatgtccg gaatcggcca ccaccacaga aggacgtgca
     6061 ttaccttttg gtcgaatttg tgcgaggaac acatctttga aatcgctgcc gtcaacgctt
     6121 tcgatgaaca gcaccgagct gccattagtc gcttgctgga attgcccttg cgccagcgcc
     6181 gccatgccag ggttcgcttt cgcttctgct aacacttcat cctgatgacg cgatgaccac
     6241 ggtcccgccc acatcacgtt aaccgccgcg acgattgccg tgaataccgc aaggatcatt
     6301 gccgctttca ccagaaccgc tttgctcaag ccgcaggcat gcattaccgt aatttcactt
     6361 tcggtataca gtttgcccag cgtcatcagc agcccgagga acaggcttaa tggcaggata
     6421 agctgcgcca tttccggcac gcccaacccg agaagggaga gcaccagatt cgccggaata
     6481 tcgccgtcaa ccgctgcgcc gaggatcctc actaactttt gacagaagaa gatcaaaagc
     6541 aagatgaaga gtatcgccag ctggcttttg agcgtctccc gcaccagata tcttatgatt
     6601 atcactttaa atacgcccgt aaaaactcgt cttttgcagg attttagctt gtttcatggc
     6661 ttaaacgtca tttattctct tgagtcgtcg aaatcgtcgc taagataatt atactcaacg
     6721 gattcacctc tcagattttg ttctgacgtg ccaatgccgt aataacgtta agattaacac
     6781 gaagtcatcg caacagcgga catgagttac gaaagcttgc aattctatct gtagccaccg
     6841 ccgttgtctt taagattcag gagcgtagtg catggagttt agtgtaaaaa gcggtagccc
     6901 ggagaaacag cggagtgcct gcatcgtcgt gggcgtcttc gaaccacgtc gcctttctcc
     6961 gattgcagaa cagctcgata aaatcagcga tgggtacatc agcgccctgc tacgccgggg
     7021 cgaactggaa ggaaaaccgg ggcagacatt gttgctgcac catgttccga atgtactttc
     7081 cgagcgaatt ctccttattg gttgcggcaa agaacgtgag ctggatgagc gtcagtacaa
     7141 gcaggttatt cagaaaacca ttaatacgct gaatgatact ggctcgatgg aagcggtctg
     7201 ctttctgact gaactgcacg ttaaaggccg taacaactac tggaaagtgc gtcaggctgt
     7261 cgagacagca aaagagacgc tctacagttt cgatcagttg aaaacgaata agagcgaacc
     7321 gcgtcgtccg ctgcgtaaaa tggtgttcaa cgtgccgacc cgccgtgaac tgaccagcgg
     7381 tgagcgcgcg atccagcacg gtcaggcgat tgccgccggg ataaaagcag cgaaagatct
     7441 cggtaatatg ccgccgaata tctgtaacgc cgcttatctc gcctctcagg cgcgccagct
     7501 ggctgacagc tacagcaaga atgtcatcac ccgcgttatc ggcgaacagc agatgaaaga
     7561 gctgggaatg cactcttatc tggcggtcgg tcagggttca caaaacgaat cgctgatgtc
     7621 ggtgattgag tacaaaggca acgcgtcgga agatgcacgc ccaatcgtgc tggtgggtaa
     7681 aggtttaacc ttcgactccg gcggtatctc catcaagcct tcagaaggca tggatgagat
     7741 gaagtacgat atgtgcggcg cggcggcagt ttacggcgtg atgcgtatgg tcgcggagct
     7801 gcaactgccg attaacgtta ttggcgtgct ggcaggctgt gaaaacatgc ctggcgggcg
     7861 tgcctatcgt ccgggcgatg tgttaaccac catgtccggt caaaccgttg aagtgctgaa
     7921 caccgatgcc gaaggccgcc tggtactgtg cgacgtgtta acttacgttg aacgttttga
     7981 gccggaagca gtgattgacg tggcgacgct gaccggtgcc tgcgtgatcg cgctgggtca
     8041 tcatattact ggtctgatga cgaaccataa tccgctggcc catgaactga ttgccgcgtc
     8101 tgaacaatcc ggtgaccgcg catggcgctt accgctgggt gacgagtatc aggaacagct
     8161 ggagtccaat tttgccgata tggcgaacat tggcggtcgt cctggtgggg cgattaccgc
     8221 aggttgcttc ctgtcacgct ttacccgtaa gtataactgg gcgcacctgg atattgcagg
     8281 aaccgcctgg cgttctggta aagcaaaagg cgcaaccggt cgtccggtag cgttgctggc
     8341 acagttcctg ttaaaccgcg ctgggtttaa cggcgaagag taagtttgcg ttttaccctc
     8401 accctaaccc tctccctaag ggagagggaa ccgatcgagc gcaatagaaa ttcattgctc
     8461 atctttcagg gtgaggaatg cagcaatatt taaatccacc acaagaagcc ccatatatga
     8521 aaaacgcaac gttctacctt ctggacaatg acaccaccgt cgatggctta agcgccgttg
     8581 agcaactggt gtgtgaaatt gccgcagaac gttggcgcag cggtaagcgc gtactcatcg
     8641 cctgtgaaga tgaaaagcag gcttaccggc tggatgaagc cctgtgggcg cgtccggcag
     8701 aaagctttgt tccgcataat ttagcgggag aaggaccgcg cggcagtgcg ccggtggaga
     8761 tcgcctggcc gcaaaaacgc agcagcagcc cgcgcgatat attgattagt ctgcgaacaa
     8821 gctttgcaga ttttgccacc gctttcacag aagtggtaga cttcgttcct tatgaagatt
     8881 ctctgaaaca actggcgcgc gaacgctata aagcctaccg cgtggctggt ttcaacctga
     8941 atacggcaac ctggaaataa tggaaaagac atacaaccca caagatatcg aacagccgct
     9001 ttacgagcac tgggaaaagc agggctactt taagcctaat ggcgatgaaa gccaggaaag
     9061 tttctgcatc atgatcccgc cgccgaacgt caccggcagt ttgcatatgg gtcacgcctt
     9121 ccagcaaacc atcatggata ccatgatccg ctatcagcgc atgcagggca aaaacaccct
     9181 gtggcaggtc ggtactgacc acgccgggat cgctacccag atggtcgttg agcgcaagat
     9241 tgccgcagaa gaaggtaaaa cccgtcacga ctacggccgc gaagctttca tcgacaaaat
     9301 ctgggaatgg aaagcggaat ctggcggcac cattacccgt cagatgcgcc gtctcggcaa
     9361 ctccgtcgac tgggagcgtg aacgcttcac catggacgaa ggcctgtcca atgcggtgaa
     9421 agaagttttc gttcgtctgt ataaagaaga cctgatttac cgtggcaagc gcctggtaaa
     9481 ctgggacccg aaactgcgta ccgctatttc tgacctggaa gtggaaaacc gcgaatcgaa
     9541 aggttcgatg tggcacatcc gctatccgct ggctgacggc gcgaaaaccg cagacggtaa
     9601 agattatctg gtggttgcga ccactcgccc ggaaaccctg ctgggcgata ccggtgtagc
     9661 tgttaacccg gaagatccgc gttacaaaga tctgattggc aaatatgtca ttctgccgct
     9721 ggttaaccgt cgtattccga tcgttggcga cgaacacgcc gacatggaaa aaggcaccgg
     9781 ctgcgtgaag atcacaccgg cgcacgactt taacgactat gaagtcggta aacgtcacgc
     9841 cctgccgatg atcaacatcc tgacctttga cggtgatatc cgtgaaagcg cccaggtgtt
     9901 cgataccaaa ggtaacgaat ctgacgttta ttccagcgag atcccggcag agttccagaa
     9961 actcgagcgt tttgctgcac gtaaagcagt cgttgctgcg gttgatgcac tcggcctgct
    10021 ggaagaaatt aaaccgcacg acctgaccgt tccttacggc gaccgtggcg gcgtggttat
    10081 cgaaccgatg ctgaccgacc agtggtacgt gcgtgccgat gtactggcga aaccggcagt
    10141 tgaagcggtt gagaacggcg acattcagtt cgtaccgaag cagtacgaaa acatgtactt
    10201 ctcctggatg cgcgatattc aggactggtg tatctctcgt cagctgtggt ggggtcaccg
    10261 tatcccggca tggtatgacg aagcgggtaa tgtttatgtt ggccgcaacg aagaagaagt
    10321 gcgcaaggaa aataacctcg gtgccaacgt tgccctgcgt caggacgaag acgttctcga
    10381 cacctggttc tcttctgcac tgtggacctt ctctaccctt ggctggccgg aaaataccga
    10441 cgccctgcgt cagttccacc caaccagcgt gatggtatct ggtttcgaca tcatcttctt
    10501 ctggattgcc cgcatgatca tgatgaccat gcacttcatc aaagatgaaa atggcaaacc
    10561 gcaggtgccg ttccacactg tttacatgac cggtctgatt cgtgacgacg aaggtcagaa
    10621 gatgtccaaa tccaagggta acgttattga cccgctggat atggttgacg gtatttcgct
    10681 gccagaactg ctggaaaaac gcaccggcaa tatgatgcag ccgcagctgg cggacaaaat
    10741 ccgtaagcgc accgagaaac agttcccgaa cggcattgag ccgcacggca ccgatgccct
    10801 gcgcttcacc ctggcggcgc tggcgtctac cggtcgtgac atcaactggg atatgaagcg
    10861 tctggaaggc taccgtaact tctgtaacaa gctgtggaac gccagccgct ttgtgctgat
    10921 gaacacagaa ggtcaggatt gcggcttcaa cggcggcgaa atgacgctat cgctggcgga
    10981 ccgctggatt ctggcggagt tcaaccagac tatcaaagcg taccgcgaag cgctggacag
    11041 cttccgcttc gatatcgccg caggcatttt gtatgagttc acctggaacc agttctgcga
    11101 ctggtatctg gagctgacca agccggtaat gaacggtgga acagaagccg aactgcgcgg
    11161 tactcgccat acgctggtga ctgtactgga aggtctgctg cgcctcgcgc atccgatcat
    11221 tccgttcatc accgaaacca tctggcagcg tgtgaaagta ctttgcggta tcactgccga
    11281 caccatcatg ctgcagccgt tcccgcagta tgatgcatct caggttgatg aagccgcact
    11341 ggccgacacc gagtggctga aacaggcgat cgttgctgta cgtaacatcc gtgcagaaat
    11401 gaacatcgcg ccgggcaaac cgctggagct gctgctgcgt ggttgcagcg cggatgcaga
    11461 acgtcgcgta aatgaaaacc gtggcttcct gcaaacgctg gcgcgtctgg aaagcatcac
    11521 cgtgctgcct gccgatgaca aaggtccggt ttccgttacc aagatcatcg acggcgcaga
    11581 gctgctgatc ccgatggctg gcctcatcaa caaagaagat gagctggcgc gcctggcgaa
    11641 agaagtggcg aagatcgaag gtgaaatcag ccgcatcgag aacaagctgg caaatgaagg
    11701 ctttgtcgcc cgcgcaccgg aagcggttat cgcgaaagag cgtgagaagc tggaaggtta
    11761 tgcagaagcg aaagccaagc tgattgaaca gcaggctgtt atcgccgcac tgtaatcaac
    11821 acagtgatga aaacgaaggc cggagcatgc tccggccttt tttatctctt acagaaatgg
    11881 taaagaaggc ataactcccc gagaaatact ggctaagaag cctttatctg gttgttcttc
    11941 tttattgatt aaaggaaggt catccaaatc gcccagcaaa tacgtatttt ttgcctgcca
    12001 gtcaatcatc catattttca gcagtgggta agccagacca cccgtaatcc cggatatcac
    12061 caccaacgca cacatgcgat aaagcatacc gtggtacgtt aaagttgagc gaaaacgaat
    12121 acgcccatca ttcagtgaca ggttgctcat aaaatgattt cgcaacgaca ccgttaaata
    12181 actggtgcta acagcaatcc caaaataata aattaactgc gcgattatca ttttacgttg
    12241 catttccata aattgctgta aattctcaat atcatcattt gcatacccag aactatcata
    12301 ctcatttaat atttgatcga agataatata accagcaaca gcgagaaaag gtaataaagc
    12361 taaaaatgcg aggatggcat attttataca atacgcagta tctaatttca ccttgaaacg
    12421 atgtataccg aaactggtat tgctccagag aaaactcatt accaggctat ataaagtacc
    12481 attaaaaata ccaatggaaa caataccaac tattgccatc agaaccacag atataataac
    12541 actacttgaa ctattggcat gtagcatctt tgtcgagata aagaaaacag tccccatccc
    12601 aatggccatt aaaatcggca agaaaaaggt tacccaccag aaccctttca aagagcaatt
    12661 aaaactaaat cttacgccgt tgagactggt cattaaggcc tgataacgca gtccttttgc
    12721 tgccataaat ataagcaaaa ccaacagtaa caaagtcaaa acacagccaa caagcggcat
    12781 atctgctgac actgtcatta agattgcgaa atagaaaaaa acaaaaacaa gacaactaac
    12841 aaaaacattc ccaccggtaa ttccataaga aaatcgttgt ccgttaactt ccatattagc
    12901 ataaagataa cgcttacatt tcattaatgc ccatggtaaa tagataccta gcgttataat
    12961 cgttaacaac acattcacca cacaaataag aaaatatctc tcacctgtac catgaaaaac
    13021 aaaagaatgg gacggaacat ccatttcatt aataacttga gccataaaaa tcccctgaat
    13081 taaaaaatcc attgatataa tttatagata cagacagtaa aatgttctgc cacgttacaa
    13141 tactcttatc agtaataggc tggcaaaaac tttattgctt tataagacgc ttgcgcacgt
    13201 aagaagagag tggtattaaa caaatgtata attactttat ttttattggg ttaaactatg
    13261 aataacattg cgccgcaatc acctgtaatg cgtcgcctga cgctgcagga taatcctgct
    13321 atcgccagcg tcatccgtca ggtttacgca gagtatggtc ttaccgccga taaaggctac
    13381 accgttgccg atccgaatct cgacgaactg tatcaagtgt acagccagcc tggccatgct
    13441 tattgggtgg ttgaatacaa tggcgaagtg gtcggcggcg gcgggattgc gccattaaca
    13501 ggtagtgaag cggacatttg cgaactgcag aaaatgtact ttcttcccgc tattcgcggt
    13561 aaagggctag cgaaaaaact ggccttaatg gcgatggagc aggcacgaga gatgggcttc
    13621 aaacgctgct atctggaaac gaccgctttt ttaaaggaag ccattgcgct ttatgagcat
    13681 ttgggctttg agcatatcga ctatgcgctt ggctgcacgg gccatgtcga ttgtgaagtg
    13741 cggatgctac gcgaactcta aaaaaatgcc cgctagtatg tgctatcggg cattttaatc
    13801 aattaattag tggcgaaccc cgtcatcgtc ttcatcgaca aaatcttcat cgtcgccatc
    13861 ttcgccgtta ggatcttcaa agtaagtgcc ccaaccgtcg tactcaacgt caaatttctc
    13921 cgccagcgtc atcagttgtt caacctgggc atcgatcaga tcggcattca acgcgcactc
    13981 gctgaggatg tcgcagcaaa tcacgatatc accatcttca acttccagct cttctggatc
    14041 ggtcacttcg taaccgagtt taaacgcttc aactgccgct ttttccagag tttccagatc
    14101 gtctgcggaa agatggtgtt cgatggtgta cagcgcgtcc ggatcgctgc catcttccag
    14161 taattcttca ataatcaaac gtgtttcttc acgctgttct tccagttgtt ccgggtttgc
    14221 catggctcat tcctcttaat gtcctgccga tactcttatt gtcacacacc actgacattg
    14281 cctccacctt tgtgacaaag atttatgctt tagacttgca aatgaataat aatacacata
    14341 aattgagttt taattcattg aggcgttagc cacaggaggg atctatgtcc gggttttatc
    14401 ataagcattt cctgaaatta ctcgatttca cgccagctga actcaacagc ctgctgcagt
    14461 tagccgcgaa gctgaaagct gataagaaaa gcggtaaaga agaagccaaa ctcactggta
    14521 aaaacatcgc gctcatcttc gaaaaagact cgactcgtac ccgatgctct ttcgaagttg
    14581 ccgcatatga ccagggggct cgcgttactt atcttggccc aagcggtagt cagattggtc
    14641 ataaagagtc gattaaagac actgcccgcg tgcttggtcg catgtatgac ggtattcagt
    14701 atcgcggcta tggtcaggag attgtcgaaa cactggcgga atacgctggc gtgccggtat
    14761 ggaacggcct gaccaatgag tttcacccca cgcagttgct ggcggatctc ctcaccatgc
    14821 aggagcattt acccggtaaa gcattcaacg aaatgacgct ggtctatgca ggtgacgcac
    14881 gtaacaacat gggcaattcg atgctcgaag ctgcggcgct taccggtctg gatttgcgtc
    14941 tggtcgcgcc acaggcatgc tggccagaag ctgcgctggt tacggaatgc cgcgccctgg
    15001 cacaacaaaa tggcgggaat attacgctga cggaagatgt agctaaagga gttgaaggtg
    15061 ctgactttat ctataccgat gtatgggtgt cgatgggtga agcaaaagaa aaatgggctg
    15121 aacgcattgc attactgcgt gattatcagg tgaacagcaa gatgatgcag ttgaccggta
    15181 acccggaggt caaattcctc cattgcctgc ccgcgtttca tgacgaccaa acgacgcttg
    15241 gcaaaaaaat ggcggaagag tttggcctac atggcggaat ggaagtgact gatgaggtct
    15301 tcgaatctgc cgccagcatt gtatttgatc aggcggaaaa ccgcatgcat accatcaaag
    15361 cggtgatggt cgcgacgctc agtaaattaa ataattaata
//
LOCUS       DAJTQQ010000089        15344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000089 DAJTQQ010000000
VERSION     DAJTQQ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.089"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(172..1197)
                     /gene="tdh"
                     /locus_tag="OO309_003115"
     CDS             complement(172..1197)
                     /gene="tdh"
                     /locus_tag="OO309_003115"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR5848169.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="OO309_003116"
     CDS             complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="OO309_003116"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR5848170.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="OO309_003117"
     CDS             complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="OO309_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR5848171.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3839..4771
                     /gene="rfaD"
                     /locus_tag="OO309_003118"
     CDS             3839..4771
                     /gene="rfaD"
                     /locus_tag="OO309_003118"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR5848172.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4781..5827
                     /gene="rfaF"
                     /locus_tag="OO309_003119"
     CDS             4781..5827
                     /gene="rfaF"
                     /locus_tag="OO309_003119"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR5848173.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5831..6823
                     /gene="rfaC"
                     /locus_tag="OO309_003120"
     CDS             5831..6823
                     /gene="rfaC"
                     /locus_tag="OO309_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR5848174.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6820..8028
                     /gene="rfaL"
                     /locus_tag="OO309_003121"
     CDS             6820..8028
                     /gene="rfaL"
                     /locus_tag="OO309_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR5848175.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8064..9206)
                     /locus_tag="OO309_003122"
     CDS             complement(8064..9206)
                     /locus_tag="OO309_003122"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCR5848176.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9215..10228)
                     /locus_tag="OO309_003123"
     CDS             complement(9215..10228)
                     /locus_tag="OO309_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCR5848177.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="OO309_003124"
     CDS             complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="OO309_003124"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR5848178.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="OO309_003125"
                     /gene_synonym="rfaI"
     CDS             complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="OO309_003125"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR5848179.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHIFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="OO309_003126"
     CDS             complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="OO309_003126"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR5848180.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12826..13950)
                     /locus_tag="OO309_003127"
     CDS             complement(12826..13950)
                     /locus_tag="OO309_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR5848181.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="OO309_003128"
     CDS             complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="OO309_003128"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR5848182.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         4381 a         3444 c         3145 g         4374 t
ORIGIN      
        1 tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca
       61 gctatataaa agatcagcgc aattacgaac tctttattac actgacagga ttttacataa
      121 ttaacagatt atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca
      181 gctcagaata actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc
      241 atcgatagag aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc
      301 cgccatcttg taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc
      361 tttaaagatc actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc
      421 aatacgaccg ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga
      481 catttccaga ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag
      541 gttttctttc gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg
      601 gtattcgtta acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc
      661 agccataata cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa
      721 cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga
      781 gatattgtcg gggattttga aagcgttgaa cgccggaatc accagatatt cggcgaagca
      841 gcccggacgg ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg
      901 acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt
      961 gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac
     1021 catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt
     1081 cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga gttccggtac
     1141 agggacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc
     1201 acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg
     1261 cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt
     1321 cggaacgacc ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc
     1381 aaatttctgc gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc
     1441 cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag
     1501 acggtcacgc aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac
     1561 aattgctggt gccagcgagt tggagaacag gtacggacga gaacgctggc gcagccactc
     1621 aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag
     1681 cgtgccggtg atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc
     1741 attttcaccg acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt
     1801 atctgccaga tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc
     1861 atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc
     1921 aagctcctgc atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc
     1981 atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag
     2041 cgtttcaaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat
     2101 ccccaggaat gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat
     2161 aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat
     2221 cagatccgga tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct
     2281 tccatcagcc acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa
     2341 caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg
     2401 cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg
     2461 acgagatgat gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc
     2521 tctcaacacc tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg
     2581 attttactgc attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca
     2641 gttggcatca aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc
     2701 tcagcgcctg catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc
     2761 attttcctct ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga
     2821 caaaaatgcc ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca
     2881 gaactaattt atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa
     2941 tatatacatg attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc
     3001 ctttttttat agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca
     3061 atcctcgagt tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg
     3121 ttttcactaa acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca
     3181 aaacatactc tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg
     3241 taaatgactc atctttttga attttttcga tacgacttct gatatatcta aatttctttt
     3301 ttatatcgat aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt
     3361 tcaaatcggg agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt
     3421 aactaaaata aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc
     3481 aatcccctct accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta
     3541 ataagaccat taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta
     3601 tattatccat tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac
     3661 tattcacatg caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt
     3721 tgcaattagc atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat
     3781 gattacagac attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat
     3841 gatcatcgtt accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga
     3901 taaaggcatc accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa
     3961 cctggtggat ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat
     4021 ggctggcgaa gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac
     4081 caccgagtgg gatggcaagt atatgatgga taacaactat caatactcca aagagctgct
     4141 gcactactgt ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg
     4201 cggacgcacc tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg
     4261 ttactcaaaa ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca
     4321 gattgttggc ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat
     4381 ggcgagcgtc gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt
     4441 cgaaggtagc gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa
     4501 cctgtggttc ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga
     4561 atccttccag gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata
     4621 cattccgttc ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac
     4681 aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga
     4741 atacatggcc tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg
     4801 cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg
     4861 ctatccccag gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg
     4921 gatgccggaa gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg
     4981 cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt
     5041 accaaactcc ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg
     5101 ctggcgcggc gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc
     5161 ctggccgcta atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac
     5221 cgcacaagat ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa
     5281 atcatatacc tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc
     5341 gggtgcggag tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa
     5401 gcagctgatt gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc
     5461 gggcaatgag attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc
     5521 gggggaaaca cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac
     5581 taacgattct ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta
     5641 tggtccaagt agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct
     5701 gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt
     5761 gatcgacatt actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga
     5821 agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac
     5881 gttgcccgca ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt
     5941 ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt
     6001 ggcaatacgt cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc
     6061 gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt
     6121 aaaaagcgcg gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca
     6181 aaccgctcgc gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca
     6241 gcagcacgcc gtagaacgca cccgcgaact gtttgccaaa agtttgggct atagcaaacc
     6301 gcaaacccag ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc
     6361 tggcgaatat gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga
     6421 acactggcga gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg
     6481 gggcgcgccg catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga
     6541 agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt
     6601 agtgtcggtg gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac
     6661 ggtttatgga ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg
     6721 tcgttctaca agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa
     6781 tcttgaaata ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc
     6841 tctctcgaga aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt
     6901 accacctatt ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca
     6961 ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca
     7021 tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc
     7081 cctgatgcag gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta
     7141 tgcacaatag caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc
     7201 gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat
     7261 tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct
     7321 gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa
     7381 taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta
     7441 tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa
     7501 caatggaagt tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc
     7561 acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat
     7621 cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt
     7681 ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat
     7741 ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt
     7801 ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag
     7861 tgtataaaaa atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc
     7921 ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat
     7981 ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag
     8041 gctgcataat gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt
     8101 tgagttacgc catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact
     8161 gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc
     8221 ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca
     8281 cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat
     8341 ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc
     8401 ttttcaggcg gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc
     8461 ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc
     8521 acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc
     8581 aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag
     8641 atattaagtt cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt
     8701 ccatttggta caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag
     8761 tataaactag ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta
     8821 tgcatatgta taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc
     8881 atgctattat gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc
     8941 aatattctct gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta
     9001 tatatgcgac tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt
     9061 gagtatcctg gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc
     9121 tgatacatcc acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta
     9181 accgtaaata ttattttatc aaccatctcg tatattaacc tttcataaca ttatatttaa
     9241 ttgctgtaaa aacaccatcg agaaattttt tctggtaaag caagtgttta tatttttctt
     9301 tgtgctcatg ttttttaaca gcttttttat aaggtatatt tctccatggt gatatattat
     9361 aaatattacg aaaataatct gcagaggcgt agttcgccca atcatgccat ggcttagtta
     9421 taccagtata atgtatgaag acagtgtcat cattaataaa ccgggtgtaa tattcactat
     9481 ttttttcttc aaattctgac tttatagtat aaatacaatt atattttctc ggtaatattt
     9541 tagcatgatg tagaaacatt aagtttaaaa tatcttgatc cgggtacttt aattttttta
     9601 taattgactc atctgataat agatcaaaga atttttccgt tagtctttgt tttaaccatt
     9661 cccgtaaatt aatatacatt acaccagagt taaaatagct accgttaaaa tcctcattac
     9721 acaaacgctc agcgctttta ctttgcatag catctacatc aattaccact gccccatatt
     9781 catctttaaa ctctaatgct attaactcgt tcaatgaacc cttacagacg atatcagcat
     9841 caagatataa taatgaatcc aatcgcgcag aaaaataatc aaatgaaagc aaacgataat
     9901 aaatagatac tggccagata tttgattgag gtagcgcctt caatggctca gaatcaatat
     9961 gatataattt aattactgtt tgatacttcg ttgctaattg tgataataat tcaatatact
    10021 ctttgtctaa gtaatcagcg attatgtgaa aggtaaattt gttttgttta ttcttttcaa
    10081 gaattgaagc aattgaaatc gcagccccaa cctggtagtt ctcatcaata ccccatgaga
    10141 cattaaacgt ttcccgttca tcaagtttaa cggggcgctt gtccagttca attatatctt
    10201 taaattgagt aagatgttta aaatccaatt taattaccct tcatcataat cattatttgc
    10261 tcttcaatcc tagtttgact tttacatccc tgattacttt tttaattttc tttttgaata
    10321 taagatatgt atagccaaag tctttcaatt cattttttat attatagtgc cgttcaagat
    10381 caatgcgatc ttttgctttt aaaacagcgg ttgttttttt acctgaaagg tcaattagtc
    10441 ttaaaccttt ttcggatact ataaaatttc ctttatgagg atcgccagac accattccat
    10501 gctgatgcaa ctcttttatc gattctgata attgatcctt cagatcttca gaaatttcca
    10561 aatactcatt taacccgaca ccttcaatat actcaatgag cataatataa taatgagcga
    10621 aatttaatgt tttacgttca gctagcaaaa aatagtcatt aattgattgg atgccttcac
    10681 cacgtacccg atcggtttga taaattaaat tttcataata atcttttttt atgcaagatt
    10741 ttaagaaacg ttcggtcatt ttatgctttg gcgcataaac tttaagtaca agaggtccac
    10801 gagcagtgtc aataagaatg actttagtat catcaataga acgaaaaact tttaatgttt
    10861 taaccctgca agttaaaaag tcatttagaa cttgctcata aaagggatca ttatctttta
    10921 tgaaaacttt caacccattt atcgttttat tatatatcat aatgaattat tttaacctta
    10981 aatctttaga agcatttttc tttataatac tttaaataat taaaaatgcc tgccatataa
    11041 tgcttttgtt taaatttatg ttttgcacaa taccgatact gattgctatt tacaggttta
    11101 agtaaatctt ctttactcca cggagaagct gctttggcga tcaaaaaact acgtgaaacc
    11161 ggataatcag cccactcatg ccagggtttt gtaggtccca catagtgtat aaacacggtg
    11221 tcatcattga ctggattatc aactttgtct tttaattcat agttaatact atatcgggta
    11281 ttatatttct ccgaaataaa cttcacttta ccattcaata atacattcag tacatcttga
    11341 tcaaggtggg tgattttact tacccaatct gggtcacgca acatttcaat agcttttgac
    11401 gaaatgttat ttagattcca ctcatcaata tttatcagta aaaaaccagc attgaaatat
    11461 ccagaagcta actgtggtgt agttaatacc gaggctcgat tttgccacca ttcaatatct
    11521 ctttcagcta caactgcggc aatttcatta gtagaaaatt gataatctaa gagttcttta
    11581 atactaccct tgcaagcaat atctgcatca agatatagta ttttttcatg tttatgatag
    11641 aaataatctg caattataaa tcgaaaatat gttgcgtaag tccagttttt cgtgcttggt
    11701 aatgacttca gcttatcaca attgataaca taaatattaa ttcttgaatt atattgtttt
    11761 gctaaatcag aaaaatataa tttgtcttta tcactgatat aatctgtgaa aatatgaaat
    11821 tcacaagaga tttctctatt gtttaataga atagatgcga tggctacacc acatccaaaa
    11881 agaaaatttc tgtcaatacc aaatgctata tcaagagtat ttttagatct tatattttga
    11941 taatgaaaat tatactcaaa aaggatgatg tcactatcat tgagttgaga catatttatt
    12001 ctgtatccaa aattttggca aaaataagtt acgggttaca acgattttcg aatcgttctt
    12061 tccctgattt ttgtggcttt tgcttctgct tgcgacagca gtccttgttc ctgcttgaga
    12121 atgtctttaa gcggggcggc aaaatacacc ttcataaagc gccagatatc ccgctgagtc
    12181 aggccaatat tcattgaaga aaaataaagc cctataagat ctttatcgcg ccaacgacct
    12241 ggaacgcgcg tgcgaagctg cgcccggtgt aggtcaatta ccgaaatttt taactcctct
    12301 tccttaccgg aaaaaggcaa gtgcagcagg aaatgacaga tataacagtc acgatggtta
    12361 atgcccgcag catgcatatc gcgcaccatc gtcgcgacac gcttaataag catacgcttt
    12421 acgcgaacat ctggtgggtt ggtcgcccag tcagcacagt aatcttccag actgatggtt
    12481 ggtgtcagat cttcggtaat aataaacgag gttctggtca gcggattcat gcctttttcg
    12541 ccgaatgcca ccccatacat agtatcaacg ccgacatccc gcagtcgatg aatcgcattc
    12601 cattcgcggt ctgcgcctaa tactggcatc cgcaatgaga gtaaattttt gattatctct
    12661 ttcagggtcg tgccgcgatg ccatttgaga aaatagcttt ttcccgccat ttcaaagcgc
    12721 agagtacggc gagtttccag ttcacgaaat acctcaccct gcaaggtttt aacttcctca
    12781 aaaggatctt tgccgcgcca taacgtggca aacggctctt taagttcaac catccagacc
    12841 acccgttatg atatccgccg ctttctctgg cagactgtat aaatcttgtg tatcagcata
    12901 atgtcgcgca ttttccgccc aagcctggcg caatgaagat tgcgttaacg ctttgcgtaa
    12961 aatctcattc aatgtttcct ggcggaatgg ctcagcaata gcctcgccgc aattagcgtc
    13021 gacaatataa tgcgcatagc cacaaacggc agttgttagt accggtaatc ctgcagtaat
    13081 cgcttccagc agcacaattc ccgccgcttc ctggtaggca ggatgcagta ataaatccgc
    13141 cgccgccatt aattccgaga catcgttgcg ccccgagaag aagtgaacat tactgcgcac
    13201 gccgcgtttt tctgccagtg cctcaaattt tcgcggttta tcctgcccaa caacatatag
    13261 caatgtgttg tggcgcagcg aatccggtaa cgaagcaagt gcttcaatgg aacgatcggc
    13321 acctttacgc gtgaagtctg aaccgacctg caacaataaa tattgttgtt cggttattcc
    13381 attcttctta cggaagattt cacggctatt ggctggctgc tggctatatt tacgatcagg
    13441 ataaatcccc ggtggcagaa tatgaaaacg ctccgcttca gtctgataat gtttctggaa
    13501 atcggcgatt tgcttatctg tcagcatcag cagctgtgtc ggcttgccct gttcgaaggt
    13561 tgcccgctca aaggcggcat aatggcgata acgtgacgtc aggcgataga aaaagccttt
    13621 ttcctgcgct actttctcgg cataacaaac atcagcggca taataaacgt ccagccccgg
    13681 cattttgttg aatccaacga ctttatcgac gggatgttcg cgtaaatgtt tttgcaccca
    13741 ggcaaaatac tccgcattgc gcccgtgatt ggtatgcgat ttaaccggca ctttgatcac
    13801 ttcaaataca tcagggcatt cgccttccca cgactgggta taaacccgaa catgatgacc
    13861 tcgggcggcg actgtctgag caatacgcat aaaatcgcgc tgcaaaccgc caaagggaaa
    13921 atatttatat aaacaaaaag caacgatcat aacgacgtac ctgtcgtgga ggagggcagt
    13981 aatttatcga cagcagcaat gacatctgcc gccggaataa ccgaaagata catctcattt
    14041 cggtcacgct gatcgcgcgt tggcatttcc cggtaatctc ccgcccagaa ttgaatcatg
    14101 ttatttgacc agggacgcca gaaaatatga tctgttgcgc caaacagcga tatcagcggc
    14161 gtattaactg cagcggcaat atgcgccggt gcggaatcaa cgccaataaa cagctgcgca
    14221 tgatcgatta acgcaccaag ttccgggaag gtcacctttc cagccagcgc cgttactggt
    14281 ggcgtctggc atccctgcgc aatttcattg acgcaggcca gatcgtcttt atctgggccg
    14341 gacgtcagaa cgacttcata accacgagca tgtaaggcat caatcacagc ggaaaacttg
    14401 gcgttgtccc agcatttgaa gatttgccgc gccgtaggtt ggataaccac ataattttgt
    14461 ccaacaccag cgtgatcaag ttcgcgacgc atacgtttcc agcttgcagg cgggtaactc
    14521 atggttgtct gcttcaccaa cgaatcaagt cccaatggtg tcagcacgga taagttactt
    14581 tccaccacat ttccaccctg caacggcacc aaatgggtga aacttttacg ccaaaaagca
    14641 gactgccgat gatgataatc ctgggaaatt ttcacacgag catttaataa gcgaaccagt
    14701 atagcaacca tccattgatc ggtaagattg acgataaggt catacttatt ggcacgtaat
    14761 accttgatga gatgaaaaaa gttggcaatt ttttctgagg cttttgcttt tttatttttt
    14821 atgccgtaga gcgcgttaat ctctggattt tcagacagga tcgggatggt gtcctgataa
    14881 agcagcacat cgatttttgc gtcagggtaa ttttttttca gagaactaat gacgggagta
    14941 gttaataaca tatccccatg aaaacgcatc ttaatgagca aaattcttcg aaatggctta
    15001 tccacaagta actctttttg ttgagattat ccgattaagg tgaagcagaa aaagcacgct
    15061 accgccccag gctcaacagc tacctgaata ctgataccgc actgatattg acgcgctagt
    15121 gtatcacttc tttcgcggca atccgacccg aatgattttt aagcacattg ttgattttca
    15181 ataaagaaat caatcaaata aaatcattag caaccatatt ctttcactgt tgctctgcga
    15241 ttccttcctg cacgtacagc agaaatcaat gaatcgcata aaaatacaaa aaaagtatag
    15301 atgttcaaca agaattggcg ggtaaattgc tatttaagtc aaaa
//
LOCUS       DAJTQQ010000090        14422 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000090 DAJTQQ010000000
VERSION     DAJTQQ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14422
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.090"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..133)
                     /locus_tag="OO309_003129"
     CDS             complement(<1..133)
                     /locus_tag="OO309_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5848183.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     "
     gene            316..459
                     /locus_tag="OO309_003130"
     CDS             316..459
                     /locus_tag="OO309_003130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848184.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(678..2216)
                     /gene="aldB"
                     /locus_tag="OO309_003131"
     CDS             complement(678..2216)
                     /gene="aldB"
                     /locus_tag="OO309_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR5848185.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2381..3532)
                     /gene="yiaY"
                     /locus_tag="OO309_003132"
     CDS             complement(2381..3532)
                     /gene="yiaY"
                     /locus_tag="OO309_003132"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR5848186.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(3722..5566)
                     /gene="selB"
                     /locus_tag="OO309_003133"
     CDS             complement(3722..5566)
                     /gene="selB"
                     /locus_tag="OO309_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR5848187.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(5563..6954)
                     /gene="selA"
                     /locus_tag="OO309_003134"
     CDS             complement(5563..6954)
                     /gene="selA"
                     /locus_tag="OO309_003134"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR5848188.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(7052..7660)
                     /locus_tag="OO309_003135"
     CDS             complement(7052..7660)
                     /locus_tag="OO309_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR5848189.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(7731..8816)
                     /locus_tag="OO309_003136"
     CDS             complement(7731..8816)
                     /locus_tag="OO309_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5848190.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(8819..9181)
                     /locus_tag="OO309_003137"
     CDS             complement(8819..9181)
                     /locus_tag="OO309_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5848191.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            9718..11631
                     /gene="mtlA"
                     /locus_tag="OO309_003138"
     CDS             9718..11631
                     /gene="mtlA"
                     /locus_tag="OO309_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR5848192.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            11726..12874
                     /gene="mtlD"
                     /locus_tag="OO309_003139"
     CDS             11726..12874
                     /gene="mtlD"
                     /locus_tag="OO309_003139"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5848193.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            12874..13461
                     /gene="mtlR"
                     /locus_tag="OO309_003140"
     CDS             12874..13461
                     /gene="mtlR"
                     /locus_tag="OO309_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR5848194.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(13473..13682)
                     /locus_tag="OO309_003141"
     CDS             complement(13473..13682)
                     /locus_tag="OO309_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848195.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            13966..14328
                     /locus_tag="OO309_003142"
     CDS             13966..14328
                     /locus_tag="OO309_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR5848196.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         3524 a         3842 c         3624 g         3432 t
ORIGIN      
        1 gaatggcgtc ggcatgagga tgattacgtt ttttagcaat caggtaggga atatcatgta
       61 aaataataat cccatagaat atcaccagaa atacaaaaat aagcactccc agtgcaaaat
      121 agtccaggaa catatttccc tcaaagaata taaaaaagaa caattaacgc atattatgaa
      181 atgccatgat gcagtgcaat ttccttattt atataacgac atattgtttt ttatatactt
      241 tctgaaatgt gctgtctggt ttttcaacag gacgaggttt atcatattga aatagtggaa
      301 ctattggcca aactaatgaa taactccagt aaaacatcca cagtacagat taagcgtatt
      361 aaaccttcaa ttatctaccg tttattgctg attggcctcg gatcaccaat ggtgatttac
      421 ggcctggttc gcccgctcac catcgaaacg cgagattaaa ctcctgacaa aacacgcccc
      481 agaaacgcac ttctgttggc gtagtctggg ttattgcgca cgtaggttta aaacgtaaat
      541 tgtaacaacg tgcgctttgt ttatgccgga tgcggcgtgt acgccttatc cggcctacgg
      601 tcaggttccc gtaggcatga taagacgcgt aagcgtcgca tcaggcaatg aatacccaat
      661 gcgaccagct tcttatatca gaacagcccc aacggtttat ccgagtagct caccagcagg
      721 cacttggttt gctggtaatg ctccagcatc atcttgtggg tttcgcgacc gatacctgat
      781 tgtttgtagc caccaaacgc agcatgtgcc gggtaagcgt gataacagtt ggtccacacg
      841 cgcccagcct gtatgccgcg ccccatctta taggccagat taccgttgcg gctccagacg
      901 cccgcgccca ggccatattg cgtatcgttc gccagctcca gcgcttcttc catcgttttg
      961 aaggtggtca ccgccagcac cgggccaaaa atctcctcct ggaacacccg catattgttc
     1021 tgaccaaaca gaatcgtcgg ttcgaggtag tagccgtctt tcagttcacc ttccagcagc
     1081 ttgcgccgcc cgcctgtgag cacgtcagcg ccctcttttt taccgatatc aatgtagttg
     1141 aggatggttt ccagttgccc gtgagaaacc tgcgcgccca tttgcgtcac gctgtcgagc
     1201 gggttaccgc tgcgaatgct ttcgacacgg cggatggcgc gttccataaa gcgttcgtag
     1261 attgactcct gcactaaggc acgactcgga caggtgcaaa cttcgccctg gttaaaggca
     1321 aacagtgcaa agccttccag cgctttatcg aaaaaggcat cttcttcatc catcacatca
     1381 gcaaagaaga tatttggcga cttaccgccc aactccagcg tcaccggaat aatgttttgc
     1441 gttgcgtatt gcataatttg ttggcccact tccgttgagc cggtaaacgc cactttggcg
     1501 atgcgtttcg aggtcgccag atattcgcca atttccccac ctgcgccatt gaccacgttc
     1561 accacgcccg gcggcagtaa atcaccgaca atttccatta gcagcagtac agaaagcggg
     1621 gtaagacgtg cgggtttcag caccacacag ttgcccgccg ccagcgcggg agccattttc
     1681 cagctcgcca tcagcagcgg gaagttccac gggataatct gccccaccac gcctaacggt
     1741 tcatggaaat gataggccac ggtttcgcta tcaacttcac tgatcccacc ttcctgcgcc
     1801 cgaatacacg aggcgaaata gcggaaatgg tcaatcgcca gcggtacatc cgcagcactg
     1861 gtttcgcgaa tgggtttgcc gttatcccag gtttcagctg tcgctaacag ctcgaggttt
     1921 tgttccattc gatcggcaat cttaaacaga atcgccgcgc gatcctgcac cgaggtgtgc
     1981 gcccatttat ctttcacttt gtgcgcagca tccagcgcca gatcgatgtc tcgtttgccc
     2041 gaagacgcca cttcgcacag cagctgcccg gtcaccggcg tcagattctg gtaatactca
     2101 ccatcggcag gggctaccca ttcaccgcca ataaagttgt cgtagcgggc ttttaacttg
     2161 atggggaaac catactcgcc gggcttaatc tgtgctgaag ggggattatt ggtcatgatc
     2221 gtctccttgc ggtgtgaggt ataacaaggg tagacgtgac tggcgaaatc ttcgccagtc
     2281 ggtaacagct ttacgacagc tatcacgaat ttacgggcaa ggattacaga gatagttgaa
     2341 aaagcggctg acaatttgcc agccgttgtt ggaatgatga ttacattgcg gcgcgataaa
     2401 tcaccacaat ttcttcgtga gttgcctgga tcgggttagt aaagccacag gcatctttca
     2461 gggcattagt cgccagaacc gcgaaatctt cttctttcac gttcaggtcg cgtaggcctg
     2521 ccgggatatc cactttcttc gccagttcac ggatggcgtt aatgcaggct tcagcacctt
     2581 ccgcgtcgtt tttacctgtc acgttcacgc ccattgcagc ggcacagtca cgcagacgtg
     2641 cagcggcgac tttgctgttg aatacctgaa catgcggcag cagaacggcg ttacatacac
     2701 cgtgtggcag gttgtagaaa ccgcccagct ggtgcgccat cgcatgaaca taacccagag
     2761 aagcattatt gaacgccata ccggcaagga actgggcata agccattgct tcacgcgctt
     2821 tcgcattact gccatcttca acggctaacg gcaggttttc ggcaatcatg gtcacggctt
     2881 tcagtgcaca agcgtcagtg atcggcgtgg cggcaataga aacatacgct tcgatagcgt
     2941 gcgttaaggc atccataccc gttgcggcgg tcagtgactt cggcatacca atcatcagag
     3001 aggagtcatt gacagaaagc agcggagtca cgtgcttatc aacaatcgcc attttgttat
     3061 gacgcgcttc gtcagtgatg atgcagaaac gggtcatttc agaggccgta cccgccgtgg
     3121 tattgatggc gatcatcggc agctgcggtt ttgcagagcg gtcaacgcct tcgtaatcgc
     3181 gaatatcgcc gccattggct gccaccagcg caataccttt tgcgcagtcg tgtggagaac
     3241 caccgcctaa ggagatcacg ctatcgcaat tattttcttt aagtaatttc aaacctgcgg
     3301 cgacgttttc cgtggtgggg ttaggttggg tgccatcata aataacgcta aaaatattgc
     3361 gttcttccag tgctttttgc acatcacccg ccatacctaa tttcgttaac atattgtcag
     3421 tgacaattaa ggtacgggta aatccataat ctgccatcat attcattgca tcagtcaatg
     3481 aatcagcgcc gatgacattc acagaaggaa taaagaaagt tgaagatgcc ataacacact
     3541 cctgaaagtg taaaaaaggg tgggcagctt actgaggatt ttcattcgaa aacatgatca
     3601 ttattattaa ttatcaaaat taatctaata aaaagcacat tgtattcatt aaacaatgtg
     3661 ctttatttaa aatcataaga aatttgtatt aatttatttt aaaaattaat taatcatttc
     3721 cttatttttc cggaaataat aatgcgtcgc gcaataaatg atcatttcca cgacgacgcg
     3781 taaagccaat gcggtcaaaa tattccagaa tctgaattgc cagctttcgg cctacgccta
     3841 agcgatcgcg gaaatccgcc gcgcaggttg aaccacactc ctgatcgaga tcgcggatca
     3901 tattggcaaa ttcgacaatc cgatcgttac ggtaataacg atctttgacg atcgcggtaa
     3961 ttattccttg ctgcgccgcc tggcgtagcg tcaggcgcat tgcctgctcg tcggtacccg
     4021 tctcttttgc cagatcacgc acccaccacg gctcgtcacc aaacagcggc tctgcttttt
     4081 gccagatggc ctgctgctct tcgctgaagc ccgctttgtg atccggcaga tgcagccaac
     4141 cgtgatgact gaggatgtcg ccgctttcgc gcatcttttc aatcagcaac agtactagcg
     4201 cttcatcttc cattggcaac gccatacgtc gcagacgttc gcgcccaggc ccaggttcat
     4261 cgcgatgttg ctcatgataa gtcgctaatg tgtcgagaat tttccgttgc cagcgagcgg
     4321 caaccggcgc attcaacaag ctataaccag cctgaatata gccaggctgt tgcagcagtt
     4381 cgcgcatccc ttcgccgttg agctggcgcg cccaagcgaa atcaggaaga ttaaccgcgc
     4441 cgcgttgcag gtgaacggct aacgcttcgc tatcattctg tgccctggcg agggaagcca
     4501 gccattgcag atactctggt ttacgtttac cgcgacgcgg cgggttaagc atcacgacgc
     4561 gcgctccggc cagcgtgttg cgggcagaga tatcgcgcaa taccaggcgg tcgttatctg
     4621 ccagccataa cggggtgtcg aagaccagtt cagcaaggtt atcttccagc agtgaaacgc
     4681 gtcccgtgac gtggctggcg gcgtggtgaa tatgcagcgg ctgccactgg gtcagcggtg
     4741 catgggtttg aagctcgaca atcacccgtg tgaacggctc tggcggcaca tcggcaagca
     4801 gccagtcgcc acggttaatc tgctcttttt ccgcatcacc cgcgatgtca agcgcgatac
     4861 gctgcccggc attggcggtt tctgttggct ggttttgcgc atgcagcgca cgtacacgca
     4921 tcggtttatt tacaccagtc agccagagtg aatcgcctac cttcacttcc ccgcttaacg
     4981 ccgtaccggt gacgaccagc ccggcacctt ttacggtaaa tgcgcggtca atcgcgaggc
     5041 ggaaactatg ttggctggcg tgctcgcgtt ccggcaactg aagcagatgc tcgcgcaggg
     5101 catcaattcc ccgaccttcg gttgctgcgg tgataaacag ttttgcctca gcaaaaccgt
     5161 attcccgcag aacctccttt acctggcgtt caacctcatc aacacgcgct tcgtccacgc
     5221 gatcggcttt ggtcagcgcc actgtcagca tcgggttacc ggtcagctgc aaaatcgcca
     5281 gatgctcacg ggtctgtgcc atcacgccgt catcgcacgc caccaccaac agcgcgtgat
     5341 cgataccacc aacgcccgcc agcatgttgg aaagaaactt ttcatgaccg ggaacgtcga
     5401 taaaaccagg cacgcgacca tccggctgcg gccagtaggc atagccgaga tcgatggtca
     5461 tgccgcgctt tttttcttcc ggcagacggt cagcatttac gccagtaatc gcctgcaata
     5521 aggttgtttt gccgtggtca acgtgtccgg cagtcgcaat aatcatttca acaacatctc
     5581 caaaaaccgt tgctcatctt caaggcagcg taaatccagc cacaatcgtc cgtcataaat
     5641 acgaccaatc accggcactg gcaattcacg ccagcgggcg gctaatgact caaggtggct
     5701 accgcgtcca tcatggggtg taaacgttaa tgccgcgctc ggcaggcgat caaccggcag
     5761 cgaaccactg ccaatctgcg aaagacatgg cataacctgt accgcaaact ccgcgccgta
     5821 atgtgcgaca aggggagcct gtaaacgttg tgcctggatt tgaatgacct ctgcgctgcg
     5881 ggtaagcagg cgcagggtcg gtaatttttc actcagagct tcagggtgta aataaagacg
     5941 caacgtggct tccagcgccg cgagggtcat tttatccgcg cgtaatgcac gcttcagcgg
     6001 gtggctttgc aggcgggcga tcatctcttt tttaccaacg ataatcccgg cctgcggccc
     6061 gcctaacaac ttgtcgccgg agaagctcac cagactgacg cccgccgcaa tcaactcctg
     6121 cggcattggc tctttcggca aaccgtactg gctaagatcg accagcgagc cactgcctaa
     6181 atcagtcacc actggaacat ccagctcttt gccgagcgcc accagttcag cttcgtctat
     6241 cgctttggtg aacccctgaa tgctgtagtt actggtatgt actttcatca acagtgcggt
     6301 attttcattc accgcctgac gataatcatt cgcgtgcgtg cggttggtgg tccctacttc
     6361 gtgtagggtg cagcctgcct gacgcataac atcgggaata cgaaacgcgc cgccaatctc
     6421 caccagttcg ccgcgagata ccaccacctc tttcccgctg gcagtggccg ccaacatcaa
     6481 taacaccgcc gccgcattgt tattgacgat acaggcatct tccgcccccg taatacggca
     6541 cagcagctgc gccagcgccc gatcgcgatg tccgcgtccg gcgtcgtcca gatcgtactc
     6601 gagggtcact ggcgaacgca tagcctgcgc aacggcttcc accgcggctt ccgcctgtaa
     6661 agctcgccca aggttggtat gcagcacggt tcccgtcagg ttgatcaccg gacgcagcgc
     6721 gctctgcgcc tctttcgtca accgggcatc gacttcttgc gcccagtttt cacaccacgc
     6781 aggcagcgtc tggctgccac gaatcacttc tcgcgcttcg tcgagcatct gacgcaacaa
     6841 ttccaccacg cgggtgtgac cataagtatc acgcaaagaa aggaaggagc tatcgcgcaa
     6901 taagcgatca atagccggaa gttgactata gagggaacgc gtttcggttg tcataggaaa
     6961 cctggctgat caaggccctc tcacacggag aagggcgttt aacataacca cggattgtaa
     7021 cgtgagatgg gtcaggagga catatcgcgc atcaagcctt tggcggttcg gtgcgagcaa
     7081 aactttcgcg gctaaacagg ttttcgacca gtttgactaa gtgtgggcgg tcgacacacc
     7141 aacctggcgc aacgcggcgg aaattgagat atccgacagc acaggcaatg gcgatagtcg
     7201 ccagattgac cgtatcggtt ttgagtgtgc catcgaccag atatccttcc agcacatcca
     7261 gactgcggtt gattttctcc cgctggcgta acaattcatc ttcagactgc tgcgccgccg
     7321 gacgcgcctg ttcacgcacc gataccagcc cggcatccat aatgccatcc gccagcgcct
     7381 caattttgcg cacccgcaac gactccagcg gatcgcgcgg caacatcgcc ggagcgacat
     7441 tcattaactc aatatattca gcgatgatcg gcgaatcaaa ccagcattcg ccctcttcgg
     7501 tcaccagcac cggcactttc cctaacgggt taaattgcgc cacgccgtta tccgcgttat
     7561 agggcagttc attaataaat tcgaaagtta tgcccttttc taacaacaga ataaaaagtt
     7621 tgcgtacaaa cgggctggtg tagctaccga cgagtttcat gccgagtcct ttgtgcgagg
     7681 aaaaatatca gtatggctca ggctcacgca aaacacaggt gatataagtc tcaatgatcc
     7741 aaataaagat aatgcatcca actggtcatt cttagcagca ctttccgcat caccgaaaca
     7801 tggctgaaat ggtcggagta aaccgccacc tgggcgtaga tgccgtcggg taaggcatcg
     7861 atatcatcgt tagggtccag ttcaatggtt cccagcacac cgtccgtgcc gggaacgacc
     7921 gttaatgatt gcaatacccc ctgcgcctga taagaaccgc ctggcacgac aggtaaaata
     7981 ctagtcaatt tgccgtggaa cacctgccca ggtagcgcgt taaacaccac ttccgcatca
     8041 tcgccaggtt tcagacgtaa cagcgagttt tgccgaaatt gggcgacaat ttgccgtttt
     8101 tgctcgggga tgaagaccat caccggacgc agcggcaagg cagctgcgta tgtacctggg
     8161 cggatcagta cctgagtgac gtagccattg cttggtgcgc gaatgacagt ctgctcaaga
     8221 ttatattttg cttcagtaag ttgcgctctt aagctcacaa tctgcgattg ctcgccgttg
     8281 accatactgt cgagctgact ctggatctgc gcctgctccg ccaccgagcc tttcactaac
     8341 gcatcctgcg cgaggaaatt ttgccgcgca tcgtcgatgt cacgttccga gaacggattc
     8401 accgccgcct ggctgccttt caggtaacgt tgataatttt taaacagacg gtcgcgctcc
     8461 gctgaaacct gggtggtgtt ggcctgcgct tcagtgagct gcgcacgcag cgtctttata
     8521 ttatgcgtcg ccgtcatcag gtcagcctga agtctgtcaa ctcgcgcctg gtaacgaacc
     8581 gggtcgagct taaaaagcac ctcgcccttt tgaataagct gattattctt gtcagtgact
     8641 tcagtaacaa ttcccgtcac ctgtggcgtg atagggatcg ctatcactgc cttttgcgcg
     8701 gtaaaagtgt aagggtggtt gtagttcatc aacaaaatca aaccactcac cagaaacacg
     8761 cctcccagcg ccgccgtcgc cagcgccacg taagttaaaa caatcaatag atccataatt
     8821 agatctccgg gaacgtggaa tagtcagtct ttttctcggc ggcgagttga tgctccagcc
     8881 gggaaattcg gtcagaaagt gcggcgattt ccggttcagt cgctttctcc tgcgacgcaa
     8941 catgtgactg cataccccag ccacgctccg gttgatagag cgtcgcccag atccacagaa
     9001 acggccagat aacatgcaga gtaaacaggc tcacccagcc cgccgtatga atagcgtcgg
     9061 catgggggtg attgcgcttt ttggcaatca gataagggat gtcatgtatc gcgatgattc
     9121 cataaaaaat caccagaaag acgaagatca gcactcccaa cgcgaaatag tttagaaaca
     9181 tatctgcctc ggattcacgt ttatcagtgt tgttttttgg ctggcagcca gaagggagtc
     9241 aggctgatat tttgacaata atccgggttc gcgattctcg ccataacacc aaagaataat
     9301 ttttagaggt gatgagttgc ttagttacat aacgattgta tgacgaaggc ataacatgct
     9361 gtagatcaca tcaggtgaac gccgtaagaa aatatcttgt gattcagatc acaaagattc
     9421 aacaaaccat caaaacaaaa atgtgacatt actcacattt aaatgccatt tttagcgaaa
     9481 atcaccgcct tgtttctttt ttatacaggc gttttgtgat gagcgtcacg tcaattacct
     9541 ctctaccccc tatatttatg tgactgatat cacacaaaag cccgtcgact ggacagttaa
     9601 ccgattcagt gccagatttc gcagtatcta caaggtccgg ctacctctgc cgccacatta
     9661 acaaaaaacc tcgggcttcc agcctgcgcg acagcaaaca taagaagggg tgtttttatg
     9721 tcatccgata ttaagatcaa agtgcaaagc tttggtcgtt tcctcagcaa catggtgatg
     9781 ccaaatatcg gcgcgtttat cgcgtggggt atcatcaccg cgttatttat tccaacaggg
     9841 tggttaccga acgagacgct ggcgaagctg gtcgggccga tgatcactta tctcctaccg
     9901 ctgctgatcg gttataccgg tggtaagctg gtaggcggca aacgtggtgg cgtagtcggt
     9961 gccattacca ccatgggcgt tatcgtcggc gcagacatgc cgatgttcct cggttctatg
    10021 attgcaggtc cgctgggcgg ctggtgcatt aagcacttcg accgctgggt agacggtaag
    10081 atcaaatccg gttttgagat gctggtgaat aacttctccg caggcatcat cgggatgatc
    10141 ctcgctattc tggcattcct cggcattggc ccgattgttg aagccctgtc caaaatgctg
    10201 gctgcgggcg ttaacttcat ggttgtccat gacatgctgc cgctggcgtc tatctttgtt
    10261 gaaccggcga aaatcctgtt cctcaacaac gccattaacc acggtatctt ctcgccgctg
    10321 ggtattcagc agtcccatga actgggtaaa tcaatcttct tcctgattga agctaaccca
    10381 ggtccaggta tgggcgtgct gctggcgtac atgttctttg gtcgtggtag cgctaaacag
    10441 tctgcgggcg gtgcggcaat catccacttc ctgggtggta tccacgaaat ctacttcccg
    10501 tatgtgctga tgaatccgcg tctgatcctc gcagtcatcc tcggcggtat gactggcgtg
    10561 ttcacgctga ctatcctggg cggtggtctg gtttctccgg catctccggg ttctatcctt
    10621 gctgtactgg cgatgacacc aaaaggtgct tatttcgcta acatcgcggg tgtgtgtgcg
    10681 gcgatggctg tctccttcgt tgtctctgct attttgctga aaaccagcaa agtgaaagaa
    10741 gaagatgata ttgaagcagc aactcgtcgt atgcaggaca tgaaagctga gtctaaaggc
    10801 acatctccgc tatctgcggg cgatgtgact aacgacctga gccacgtacg taaaatcatc
    10861 gttgcctgtg acgccggtat gggttcaagt gcgatgggcg caggcgtgct gcgtaagaaa
    10921 attcaggatg caggtctgtc gcagatttcg gtcactaaca gcgcgatcaa caacctgccg
    10981 ccagatgtgg acctcgtcat cactcaccgt gacctgaccg aacgcgctat gcgccaggtt
    11041 ccgcaggcac agcatatttc gctgaccaac ttcctcgaca gcggcctgta caccagccta
    11101 accgaacgtc tcgttgctgc ccagcgccat actgaaaacg aagtgaaagt gaaagacagt
    11161 ctgaaagaca gctttgacga ttccagtgct aacctgttca aactgggcgc ggagaacatc
    11221 ttcctcggtc gcaaagcagc aaccaaagaa gaagcgattc gttttgctgg cgagcagctg
    11281 gtgaaaggcg gttacgttga gccggaatac gttcaggcga tgctggatcg tgaaaaactg
    11341 accccgactt atctgggtga gtctatcgcg gtgccacacg gtacggttga agcgaaagat
    11401 cgcgtactga aaacgggcgt ggtgttctgc cagtacccgg aaggcgtgcg cttcggtgaa
    11461 gaagaagatg acattgcccg tctggtgatt ggtattgcag cccgtaacaa cgagcacatt
    11521 caggttatca ccagcctgac caatgcactg gatgatgagt ccgtcatcga gcgtctggca
    11581 cacaccacca gcgtggatga agtgctggaa ctgctggcag gtcgtaagta atccaatcct
    11641 accctctcct cacggagagg gtttgggtga gggaaaagcc tcaccccagc cctctcgggt
    11701 aaaaacattg atgaaggtta atactatgaa agcattacat tttggcgcag gtaatatcgg
    11761 tcgtggcttt atcggtaaac tgctggcaga cgcgggtatc caactgacgt ttgccgatgt
    11821 caatcaggtg gtacttgatg ccctgaatgc ccgtcatagc tatcaggttc atgtggtcgg
    11881 tgaaaccgag caggtagata ccgtttccgg cgtcaatgct gtcagcagca ttggtgatga
    11941 tgtcgttgat ctgattgctc aggtcgattt agtcactacc gccgttggcc cggttgtgct
    12001 ggaacgtatt gctccggcta tcgccaaagg gctggtgaaa cgcaaagaac aaggtaatga
    12061 atccccgctg aacatcatcg cctgtgaaaa catggtacgc ggcaccacgc agctgaaagg
    12121 tcatgtgatg aacgccctgc cggaagacgc caaagcgtgg gtagaagagc acgttggctt
    12181 tgtcgattcc gccgttgacc gcatcgtacc gccttcggct tcggcaacta acgatccgct
    12241 ggaagtgacg gtagaaactt tcagcgaatg gattgtcgat aaaacgcagt tcaaaggcgc
    12301 actgccgaac atcccaggca tggagttaac cgacaacctg atggcatttg tcgaacgtaa
    12361 actcttcacc ctgaacacgg gtcatgctat aaccgcgtac ctcggaaaac tggccggtca
    12421 tcagaccatt cgtgacgcta ttctcgacga gaaaatccgc gcggtggtaa aaggtgcgat
    12481 ggaagaaagt ggtgcggtac tgatcaagcg ctacggcttt gacgcagaca aacatgcggc
    12541 gtacatccag aaaatcctcg gtcgttttga gaacccgtat ctgaaagatg atgtagagcg
    12601 cgtaggccgt cagccgctgc gtaaactgag tgctggcgac cgtctgatca agccactgct
    12661 cggtacgctg gaatacagcc ttccgcacaa gaatctgatt caggggattg ctggtgcaat
    12721 gcacttccgc agtgaagatg atccacaggc tcaggaactg gcagcactga tcgctgacaa
    12781 aggtccgcag gcggcgctgg cacagatttc cggtcttgat gccaacagcg aggttgtatc
    12841 cgaggcggta accgcttata aagcaatgca ataatggtgg accaggcgca ggacaccctg
    12901 cgcccgaata acagattgtc agatatgcag gcaacaatgg aacaaaccca ggccgttgaa
    12961 aaccgtgtgc ttgagcgtct gaatgctggc aaaaccgtgc gaagctttct gatcaccgcc
    13021 gtcgagcttt tgaccgaggc ggtaaatctt ctggtgcttc aggtattccg caaagacgat
    13081 tacgcggtga agtatgctgt agaaccgtta ctcgacggcg acggtccgct gggcgatctt
    13141 tctgtgcgtt taaaactcat ttacgggttg ggcgtcatta accgccagga atacgaagat
    13201 gcggaactgc tgatggcatt gcgtgaagag ctaaatcacg acggcaacga gtacgccttt
    13261 accgacgacg aaatccttgg accctttggt gaactgcact gcgtggcggc gttaccaccg
    13321 ccgccacagt ttgaaccagc agactccagt ttgtatgcaa tgcaaattca gcgctatcaa
    13381 caggctgtgc gatcaacaat ggtcctttca ctgactgagc tgatttccaa aatcagctta
    13441 aaaaaagcct ttcaaaagta agcaacgtct gcttactgcc cctctacctg cttcggccga
    13501 taaagccgac gataatactc cagacgttgt agatataacg gcacgctttc atcgggtatt
    13561 ccggacggaa tcgcgttacg gggaggaagt tttttcagat actcccggaa cgcctggctt
    13621 gatgccatga aatctacggc tttatcgata agaagcggaa cgttttcacc taatttgccc
    13681 atgatgatat ctccgtatta ccccgcgccg ggaatgcgcg gccgccaatt ttagtttagg
    13741 ttcccggtaa agatgacatt aaggaaaacg tgctgaatcc tcaataatca tccgctgctg
    13801 tttattatct ttttctatag atttcttaat taatcaacga attgcgtagt ttttgagcat
    13861 gttaatgatt acgttgcaga actattacaa tgtcatcagt tgtgaacaaa gcacctggtc
    13921 gcgcatacta ggggctataa atttatcttt atcagaagcc atcacatgaa agaagtcgaa
    13981 aaaaacgaaa tcaagcgtct cagcgatcgc ctggacgcca tccgccacca gcaggccgat
    14041 ctgtcgctgg ttgaagccgc agacaaatat gccgagctgg aaaaagagaa agccacgctg
    14101 gaagcagaaa ttgctcgcct gcgtgaggtt catagccaaa aactgagtaa agaagcacag
    14161 aagctgatga agatgccgtt ccagcgcgca attaccaaaa aagagcaggc tgatatgggc
    14221 aagctgaaga aaagtgttcg cggactggtg gttgtgcacc caatgaccgc gctggggcgc
    14281 gaaatgggcc tggaagagat gactggattt tctaagacta cgttttaagc ccctcagacg
    14341 actggttcga tcgtatcaac ccgcatctgg cttgccttag ctggtcaatg cggggactct
    14401 cctgtctact cgcctaatta cc
//
LOCUS       DAJTQQ010000091        14408 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000091 DAJTQQ010000000
VERSION     DAJTQQ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.091"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(213..1154)
                     /gene="fabY"
                     /locus_tag="OO309_003143"
                     /gene_synonym="yiiD"
     CDS             complement(213..1154)
                     /gene="fabY"
                     /locus_tag="OO309_003143"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR5848197.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1199..1636)
                     /gene="dtd"
                     /locus_tag="OO309_003144"
     CDS             complement(1199..1636)
                     /gene="dtd"
                     /locus_tag="OO309_003144"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR5848198.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1633..2505)
                     /locus_tag="OO309_003145"
     CDS             complement(1633..2505)
                     /locus_tag="OO309_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR5848199.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2499..3098)
                     /gene="yihX"
                     /locus_tag="OO309_003146"
     CDS             complement(2499..3098)
                     /gene="yihX"
                     /locus_tag="OO309_003146"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR5848200.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3197..3982)
                     /locus_tag="OO309_003147"
     CDS             complement(3197..3982)
                     /locus_tag="OO309_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5848201.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4016..4912)
                     /gene="yihV"
                     /locus_tag="OO309_003148"
     CDS             complement(4016..4912)
                     /gene="yihV"
                     /locus_tag="OO309_003148"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCR5848202.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5080..5976
                     /gene="yihU"
                     /locus_tag="OO309_003149"
     CDS             5080..5976
                     /gene="yihU"
                     /locus_tag="OO309_003149"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR5848203.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6000..6878
                     /gene="yihT"
                     /locus_tag="OO309_003150"
     CDS             6000..6878
                     /gene="yihT"
                     /locus_tag="OO309_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR5848204.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            6895..8136
                     /gene="yihS"
                     /locus_tag="OO309_003151"
     CDS             6895..8136
                     /gene="yihS"
                     /locus_tag="OO309_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR5848205.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTVYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8274..9176
                     /locus_tag="OO309_003152"
     CDS             8274..9176
                     /locus_tag="OO309_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCR5848206.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9375..11411
                     /gene="yihQ"
                     /locus_tag="OO309_003153"
     CDS             9375..11411
                     /gene="yihQ"
                     /locus_tag="OO309_003153"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCR5848207.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11457..12831
                     /locus_tag="OO309_003154"
                     /pseudo
     CDS             11457..12831
                     /locus_tag="OO309_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12874..14277
                     /locus_tag="OO309_003155"
     CDS             12874..14277
                     /locus_tag="OO309_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5848208.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
BASE COUNT         3487 a         3755 c         3625 g         3541 t
ORIGIN      
        1 taatatgatg agttaagcat tatctgcaat aattgccact cagctctaca aataatattt
       61 aaatgctcct gaacagtgat gtagcaagac acgtatggtg ccaacgaaga ataaaaattt
      121 ataaagttct tattcaatat aaccggaata gtcagggact gatgtaaatg accggaatga
      181 atgagagagg cagcaccgcc cctctccata agctactctt cttcgttccc gccctcttca
      241 tacgggccaa atggcttcgc gggcagaacg atatacgtgc cttcaaacac tgcacccggc
      301 gtctcgtcgc caaagatttc gacctgcatc tgcacccgtg cttttcgtcc gcgcgccaga
      361 cggtcgagat cgccgcttaa ggcaccaagg tcggctaccg catggggttt accgctaatc
      421 ggtttgctgt agcggatatg cgcatccgcc agaataatcg ttccgccgag gtggcgttca
      481 cgcagcatca gccagataag cccccaaccg gtgagcgtcg ccagcgagaa taaactcccg
      541 gcaaacagcg tatggtgcgg attctgattg ccggtttccg gcatggtggt gataaatttt
      601 tgcccggtat attgttgaat acgcacgccc attttttcac taagcgggat atgttcgtac
      661 cacgcctgtt gcagctgcgc gcaccagtcg ccgcgatgca aaatatcatc cagagtggcg
      721 acgggcttaa tcatcaaaaa atggcgaatc ggcgtggtgg ttggcgtggt gatttctccc
      781 tgattaacaa accccagctt ggcgaaaaac tccaccgcgt cttcacgggc gctacaggtc
      841 acgcgcttaa cgccttcctg acgcgccacc gactccaggg tcatcgccat cagcgttcct
      901 aaccctttgt cctgcacgtc cgggtgaacg accataaagc gaatggacgc ttcattgtcg
      961 gcattaatat acaatcggcc taccgccacc agattaccct gctcgtcgac gaccatctgg
     1021 tgatgcgcca tcgcatccca cgcgtcgcgt tccgaacctt ttggttgatg caggggctta
     1081 cgcaacattt cccagcgaaa gtgatagtaa cgctctaact cttcttctgt ttgtggaacc
     1141 tgaaggtgat acatagcgat actctctctt gtttcccgtg acaaccctgg aagctggctc
     1201 atacctgcaa ccagaatgtc acggggccat cattgaccag cgatacctgc atatcggcag
     1261 cgaagcgtcc tgtttgcgtg ttcatctctt gctggcggca gcgttcgacg aaatagtcat
     1321 ataacgcctc tgcgcgatcc ggtgatgcgc ctttggagaa acttgggcgc atcccccgtt
     1381 cggtatctgc ggcgagggta aactgggaaa ccaccagtac actgccgccc gcctgttgca
     1441 cgttgagatt catcttgcct tcggcatcgc taaagatgcg gtagccgagc acacgctcgc
     1501 acagacggtt tgctttctgt tcgtcgtcat ccttttcgac acccaataac accaaaagtc
     1561 ccgcgccaat ttcgcccgtc acttctccct ccacggtgac gctggcacgg gttacgcgtt
     1621 gaattaatgc aatcatggtt cgtcgtcttc ttcttgttca gctgcttgtt ttagtttgcg
     1681 gtattccccg agagtgacag taatttccgc gccaagcaag acgatacacc atgtccagta
     1741 gacccaaaca aagagaatgg ggatcaccgc cagcacaccg taaatgagct gatatgacgg
     1801 gaacatggtg atataaagcg cgaaaccttt ctttcctgct tcgaacagga gtgcggcgac
     1861 aaacgcgccg acaatcgcgt cgcggttagg tacgcggatg gtaggaacaa tgctgtacag
     1921 caaccagaag gagatccacg acaacagcaa cggaaaaata cgcagcacgt tgtcgatgac
     1981 agtattgaga tcgctcgccc agcgcaggga gagcaaatag gaactgatcg ccagactggc
     2041 ccctgccagc agcggcccca gcgttaaaat catccagtac acggcgaacg agtaaatttt
     2101 tggtcgcgct cgtttactgc gccagatggt attcaacgcg ctatcgatgg agtacatcaa
     2161 caataacgcg gtgacgatca gcccgcacgc cccaacggcg gtcatcttgt tggaattagc
     2221 aacaaattgt tcgatatacc gctgaataac atcgccagta gcaggcagaa agttggcaaa
     2281 aataaagtga cgtaactgaa tgctgacgtc ggaaaacatg ggaaaagcgg cgaaaagcgc
     2341 aaaaacaacg gcaaccagcg gcactaatga gagcaacgac acataggcaa ggttacctgc
     2401 cagggttgtc atgttgtcct catcaatacg ttgccagagt agttttagcc aggcccatag
     2461 tggacgggta cggtgcctgg ctttgtcctg aatggttttt agcataacac cttcgcgaaa
     2521 tagtccggga tggtggtttt atctttcacc agaatactgg taatgcctag ctgattagct
     2581 ccttctatat tatcggcgtt atcgtcgaaa aagaccgtat cgctgggtga aaaaccttcc
     2641 gcctgcaaaa catgctggta aattcgtgct tcaggtttgc gcatccccag atcttgcgac
     2701 agatagatat ggtcagcagc atcacgaatt tccgggtatt cttccggcca gaaggtggta
     2761 tgcaggcggt tggtattgga aagcaccacc acgcgatgcc cctgctcacg cagtttatgc
     2821 atgatggcga tcacttccgg gcgcagcgca acaaacaccg cctgccagcc gtgagagaac
     2881 tgctcgtagc ttagcggtag agccatctca tgacacagcg cctctgcgaa cgcttcgtcg
     2941 ctaatttccc cacgctcatg ctgatgaaac gcctccccca tatgaaaact cttcttaagc
     3001 gatgccagcg gaatacgcgt taaatcgctc caggctccca gcacacggtt aaagtcgata
     3061 tcgacaatca cattacctaa atcaaagata tagagcattt ttgcctcctt tcgcgccaca
     3121 agaaattaac tgtagcggga aaggatgggt ttggctatgt ggcaaggtat gaagccaggt
     3181 taacggtcac aaaaatctac gcgtcttcct ggggaagaat gatttcaatt tggctgtctt
     3241 gtaagcgtga ttttagcgca gcgggcggca gctcgtcggt aaagagcgca gtgacctgtg
     3301 ccacgttacc aatttcaacc gccgccgaag catgatactt agtgtgatcg gcgaccagca
     3361 gaatatttct cgcgtgcgcc atcatcgttt tcaccacgtt agcttcgttt acatcaaact
     3421 ccatcaacgc gccatcgctc tcaatcgccc caacgcttgt taccagataa tcagcacgaa
     3481 aatcagccac aaaggacgcc gctgaagggc caatgatccc gctattatga gagcgcaacg
     3541 taccgccggg caccatcact tcaaagcgcg ggttgtggta aagaatatgc gccacacgca
     3601 ggctgttggt gattatccgc aaatgattat ggttaagtaa cgcccgggca acatgctcaa
     3661 cagtcgtacc aatggtgata aatattgttg aaccatcagg aatatagtct gccaccgctt
     3721 cggcaatcgc tttttttttc tcggtttgcg aaacctcacg ctgctcgaac gccgtattaa
     3781 cgacgctgga agcccgaccc gcgccaccgt gatggcgcgt aatcaggcct tgctcgctta
     3841 atttacgaat atcccggcgg accgtctgcg tggagacatc cagcagattt gccagctcat
     3901 caatattcat atacccacgc tcggcgatca gcatgaggag ttggtcgtgc cgcgggttac
     3961 cggttagttc ggtaaggctc atgaaaattc ctcgaaaaac catcaccctg gcattttata
     4021 caaaaagtga caaaaaagat cgggtttgat cacagtcagg gatcccggct cgtccaccgg
     4081 gacgtgtgca ttttaacgcc gctacaccgc tggcgaagcg gactgactcc gctaaatccc
     4141 cacttgttgc cagcgccacc gccaaagcgc cgtgaaaaac atctcccgca cctgtggtat
     4201 ctaccacatc aactttgaag gccggttgat gctgacgccc accattttcc agccagtcgc
     4261 agcctgcgct accctgggtc acatagacat gaccatttgt gagcgtttgt gcctgtttta
     4321 gcgcactggc catctctttc acgcccgtta agcgcgccag acccggttct gaaaaggccg
     4381 cgtgatcgct taatgccacc agctcactga tatcctgcgg cgtaatgtcc ccgtccagaa
     4441 cggtcatcac acccgcctga cgggccaggg tgaaggcttt tttagcgccg tcgtgccagc
     4501 gtacatctgc cagcacaaca tcccactgag agaaatcaat ttcctccaac cactctgcgt
     4561 caggcagcag atccgggctg gggtagttaa tgattatccg ctcgcctttg gtatccacca
     4621 tgatggcgga ttgcgaagat ttcgcctggt tataccgttt ggtgtaacgg gtgttaacgc
     4681 cccaggattc cagctctgcc agcaggctgt tgcccgcgtc gtcatcacct acgcgaccaa
     4741 taaaatcgac ctgcgccccc agccttgccg ccgcaaccgc tgccgtcgcc gctggcccgc
     4801 caccaacttc cgtataattt ctcgccacgt atttaccgct ctccgtcggt aacccttcca
     4861 cgtaatagat gcgatccatc acggttatac ctacacaagc aacacgaatc atggtcattc
     4921 cttaaacatt ttgggatgcg ttcattttaa tttcaccaat tgtttaaaaa gtgacggctg
     4981 tcaatttttt gacgataaat tacaaattcg atcaaaaaca gacaagcaaa gcatgtcata
     5041 aatgacaaaa agtgacatca ctgtattcag gagagggtta tggcagcaat cgcgtttatc
     5101 ggtttaggac aaatgggttc gccaatggcg agcaatttat tgcagcaagg gcatcaactt
     5161 cgcgtctttg atgtgaacgc cgaagctgtg cggcatctgg tagacaaagg tgcgactccc
     5221 gccgccaacc cggcgcaggc cgctaaagat gccgaattta tcattaccat gttgccgaat
     5281 ggcgatctgg tgcgcaacgt gttgttcggt gaaaacggcg tttgcgaagg cttatctacc
     5341 gatgcgctgg tcattgatat gtccaccatc catccgctgc aaaccgataa attgattgcc
     5401 gatatgcaag ccaaaggctt cagcatgatg gatgttccgg taggccgtac ttctgcaaat
     5461 gccattaccg gtactctgtt actgctggct ggcggcaccg ctgaacaagt tgaacgtgcc
     5521 acaccgatcc tgatggcgat gggcagtgag ttgatcaacg ctggcggtcc gggcatgggg
     5581 atccgcgtta agctcatcaa caactacatg agcatcgcgc tcaatgcgct ttcggcagaa
     5641 gccgccgttt tgtgcgaagc cctgaatctt cccttcgatg ttgccgtcaa agtgatgagc
     5701 ggtaccgccg ccggtaaagg tcacttcacc acttcctggc caaacaaagt cctcagcgga
     5761 gatctttctc ccgccttcat gatcgatctt gcccataagg atcttggcat cgcccttgat
     5821 gtcgccaacc agctgcatgt gccaatgccg ctgggggccg cctcacggga ggtttatagc
     5881 caggcgcgcg cagcgggtcg cggtcgccag gactggtccg ccattctgga acaggtccgt
     5941 gtcagtgccg ggatgactgc caaagtaaaa atgtaacgac tggataaagg aataaatgaa
     6001 tgaataagta caccatcaac gacattacgc gcgcatcggg cggttttgcc atgctggcgg
     6061 tcgatcagcg cgaagccatg cgcatgatgt ttgccgcggc tggggcaccc gctccggtag
     6121 ccgatagcgt tttaactgat ttcaaagtta acgctgcaaa ggccctctcg ccttatgcct
     6181 cggcgattct ggtagatcaa caattctgct accgccaggt ggttgagcaa aacgcgattg
     6241 ccaaaagttg cgccatgatt gtcgccgccg atgagttcat tcctggcaac ggtattccgg
     6301 tcgatagcgt ggttattgac cgcaaaatca atccgctaca gatcaaacaa gacggtggca
     6361 aagccttaaa actgctggtg ctgtggcgta gtgatgaaga tgcgcagcaa cgtctggata
     6421 tggtgaaaga gttcaacgaa ctgtgccact cacacggtct ggtaagcatc attgagccag
     6481 tcgtccgccc accgcgtcgt ggcgataaat tcgatcgcga acaagcgatc atcgatgccg
     6541 ccaaagagct gggcgacagt ggcgctgacc tctacaaagt tgaaatgccg ctttatggca
     6601 aaggtccgca acaagagctt ctctgtgctt cacaacgtct gaatgaccat atcaatatgc
     6661 catgggtgat cctctcttcc agtgtcgacg aaaaactgtt cccgcgtgcc gtacgcgtgg
     6721 caatgacggc gggcgcatcg ggattcctgg caggccgtgc agtctgggca tcggtcgtcg
     6781 gtttaccaga caacgagctg atgctccgtg acgtttgcgc accgaaatta caacaacttg
     6841 gcgatatcgt cgacgaaatg atggctaaac gccgctaaga tgaggataaa aggaatgaaa
     6901 tggtttaaca ccctaagcca caaccgttgg ctggaacagg aaaccgaccg catctttgat
     6961 tttggtaaaa attccgtagt gccgactggt tttggctggt taggcaataa agggcaaatc
     7021 aaagaagaga tgggcaccca tctgtggatc acagctcgta tgttgcacgt ttattccgtt
     7081 gctgcggcga tgggtcgacc tggcgcttac gcgttggttg atcacggtat taaagccatg
     7141 aacggcgcac tgcgtgataa aaaatatggc ggctggtatg cctgcgtgaa tgacgagggc
     7201 gtggtggatg cctccaaaca gggctatcaa cacttctttg ctctgctggg tgccgccagc
     7261 gccgtcacaa cgggtcaccc ggaagcgcgc aagctgctcg attacaccat tgagattatc
     7321 gagaaatatt tctggagcga agaagagcag atgtgcctgg agtcctggga cgaagccttc
     7381 agcaaaaccg aagagtaccg cggcggcaat gccaatatgc acgcggtgga agctttcttg
     7441 attgtttatg acgtcactca cgacaaaaaa tggctggatc gcgcgattcg cgtggcttcc
     7501 gtgattatcc acgacgtcgc cagaaataat cattatcgcg ttaacgaaca cttcgatacc
     7561 cagtggaatc cgctgccgga ttacaacaaa gataacccgg cgcatcgctt ccgcgcgttc
     7621 ggaggtacac caggccactg gatcgaatgg ggccgtttaa tgctgcacat ccatgctgcc
     7681 ctggaagccc gttgcgaaca accaccagca tggctgctag aagatgccaa aggtctgttt
     7741 aacgccaccg tgcgcgatgc ctgggcaccc gatggagcgg acgggattgt ttataccgtt
     7801 gactgggaag gaaaaccggt ggtccgcgaa cgtgtacgtt ggcctatcgt cgaagcaatg
     7861 ggtacggtct acgcgctcta caccgtcact ggcgatcgcc agtatgaaac ctggtatcaa
     7921 acatggtggg agtactgcat taagtacctg atggactatg aaaatggttc ctggtggcag
     7981 gagctggatg cggacaataa ggtcatcacc aaagtctggg acggcaaaca ggatatttat
     8041 cacctgctgc attgcctggt gatcccgcgt atcccgttag cgcccggcat ggctccagcg
     8101 gttgcagcgg gtctgctgga tattaatgcg aaataactga tctgaaaata gggtgcgggt
     8161 tttcccctca ccctaaccct ctccccaaag gggcgagggg accgtccggt gcagtttttg
     8221 ggtttgacat cagcctcacg ggaggctgta tgtcgttaat taaggtgcct tgtatgcaaa
     8281 tcaccaatat gcattgcagt ggacagaccg tttcacttgc cgcgggggat tatcacgcga
     8341 ccattgtgac cgtcggtgct ggtctggctg aattaacctt tcagggttgt catttagtca
     8401 tcccgcacaa acccgaagag atgccgctgg cgcatctggg taaagtgctt attccgtggc
     8461 cgaaccggat cgccaacggc tgttaccgtt atcaggggca ggaatatcag ttacccatta
     8521 atgaacatag ttcaaaagcc gctattcacg ggctgcttgc ctggcgagac tggcaaatta
     8581 gtgaattaac caccaccagc gttaccctga cagcttttct gccgcccagt tacggctatc
     8641 cgtttatgct ggcttcacaa gttgtttatt cgctcaacgc acacacgggt ctttccgttg
     8701 agatcgcaag ccagaatatc ggcactgtcg cagcgcctta tggcgtgggt attcatcctt
     8761 atttaacctg caacttaaca tctgttgatg aatatctgtt ccagcttccg gcaaaccagg
     8821 tttatgccgt cgatgaacac gtaaatccga caacgcttca ccacgttgat gagctggatt
     8881 taaatttcac gcaggcgaaa aaaatagccg cgacaaaaat cgaccacacc tttaaaaccg
     8941 ctaatgattt gtgggaaatg accattaccc atccacagca ggcgctttcg gtaagccttt
     9001 gttccgatca accctgggtg caggtttata gcggtgaaaa attacagcgt cagggactgg
     9061 cagttgaacc tatgagttgc ccgcctaacg cctttaattc aggtatcgac ttattattac
     9121 tggaaccagg aaaaccgcac cgcctgtttt ttaatattta cggacagaga aaataacttg
     9181 tattgattag cagtcaaaat cattgaagaa catttcactc gctaaaggca acattatgta
     9241 tcttcaattt attaagttaa aaacagctca gcttctgtaa agtttttata tatcagtttt
     9301 atgagaatca ttttacataa gcctgtaata acaggaaggc aacaatctcg ttctcaactc
     9361 tatggagatt agttatggat acgccacgtc cacagttatt agattttcaa tttcatcaga
     9421 ataacgacag ttttacccta cattttcaac aacgtcttat tttaacccat aacaaagata
     9481 atccttgttt atggattggc tcaggtatag cggatatcga tatgttccgc ggtaatttca
     9541 gcattaaaga taaactacag gagaaaattg cgcttaccga cgccatcgtc agccagtcac
     9601 cggatggttg gttaattcat ttcagccgtg gttctgacat tagcgccacg ctgaatatct
     9661 ctgccgacga tcagggccgt ttattgctgg aactacaaaa cgacaacctt aaccacaacc
     9721 gtatctggct gcgccttgcc gctcaaccag aggaccatat ctacggctgc ggcgaacagt
     9781 tttcctactt cgatctgcgc ggcaaaccgt tcccgctatg gaccagtgaa caaggcgttg
     9841 gtcgcaacaa acaaacctat gtcacctggc aggccgactg caaagaaaat gcgggcggcg
     9901 actattactg gactttcttc ccacagccta cgtttgtcag cacgcagaag tattactgcc
     9961 atgttgataa cagttgctac atgaacttcg actttagcgc cccggaatac catgaactgg
    10021 cgctgtggga agacaaagct acgctacgtt ttgaatgtgc tgacacatac atctccctgc
    10081 tggaaaaatt aactgccctg ctgggacgcc agccagaact gcccgactgg atttatgacg
    10141 gggtaacgct cggcattcag ggcgggacgg aagtgtgcca gaagaaactg gacaccatgc
    10201 gtaacgcggg cgtgaaggtt aacggcatct gggcgcagga ctggtctggg atccgtataa
    10261 cctcttttgg caagcgcgtg atgtggaact ggaagtggaa cagcgaaaac tacccgcaac
    10321 tggattcacg cattaagcag tggtatcagg agggcgtgca gttcctggcc tatatcaacc
    10381 cgtatgttgc cagcgataaa gatctctgcg aagaagcggc acaacacggc tatctagcaa
    10441 aagatgcctc tggcggtgac tatctggtgg agtttggcga gttttacggc ggcgttgtcg
    10501 atctcactaa tccagaagcc tacgcctggt tcaaggaagt gatcaaaaag aacatgattg
    10561 aactcggctg cggcggctgg atggctgact tcggcgagta tctgcccacc gacacgtacc
    10621 tgcataacgg cgtcagtgcc gaaattatgc ataacgcctg gcctgcgctg tgggcgaagt
    10681 gtaactacga agcccttgaa gaaacgggca agctcggcga gatcctcttc tttatgcgcg
    10741 ccggttctac cggtagccag aaatactcca ccatgatgtg ggcgggcgac cagaacgtgg
    10801 actggagtct cgacgatggc ctggcgtccg tagtcccggc ggcgctgtcg ctggcaatga
    10861 ccggacatgg tctgcatcac agcgacattg gcggttacac caccctgttt gagatgaagc
    10921 gcagcaaaga gctgctgctg cgctggtgcg atttcagcgc cttcacgccg atgatgcgca
    10981 cccacgaagg taaccgtcct ggcgacaact ggcagtttga cggcgatgcg gaaactatcg
    11041 cccattttgc ccgtatgact accgttttca ccaccctgaa accttatctg aaagaggccg
    11101 tcgcgctgaa tgcgaagtcc ggtctgccgg tcatgcgccc gctgttcctg cattacgaag
    11161 acgatgcaca tacctattcc ctgaaatatc agtacctgtt aggccgcgac attctggtcg
    11221 ctccggtaca cgaagaaggt cgcagcgact ggacgctcta tctgccggag gataactggg
    11281 tacatgcctg gacgggtgaa gcgttccggg gcggggaagt taccgttaat gcgcccatcg
    11341 gcaagccgcc ggtcttttat cgcgccgata gcgaatgggc ggcactgttc gcgtcgttaa
    11401 acagcatcta atcacatccg cccgtgggga aactcatggg caaagggaga acatcgatga
    11461 gtcacatcac aacggaagat ccagcaactt tacgcctgcc ctttaaagag aaactctctt
    11521 acggtattgg cgacctggcc tctaacatcc tgctggatat cggtacgctt tatcttttga
    11581 agttttatac cgacgttctg gggctgccag gcacctatgg cggcattatc tttttgattt
    11641 caaaattctt tactgcgttt accgatatgg gtaccggcat tatgttggat tcccgacgca
    11701 agatcggtcc aaaaggtaag ttccgtcctt ttattctgta tgcgtcattc ccggtcacct
    11761 tactggcgat cgccaacttt gtcggcacac cgtttgatgt caccggtaaa acggtgatgg
    11821 ccactattct gtttatgctt tacggactgt ttttcagcat gatgaactgc tcctacggcg
    11881 caatggtccc ggcaatcacc aaaaacccca acgaacgcgc ctcactggcg gcatggcgtc
    11941 agggcggcgc tacgctgggc ctgctgctgt gcacggtggg attcgtgcca gttatgaatc
    12001 ttatcgaagg taatcagaaa cttggctata tcttcgccgc cacgctgttt tcactgttcg
    12061 gcctgctgtt tatgtggatc tgctactcag gcgtgaaaga gcgttatgtc gaaacccaac
    12121 caaccaatcc ggcgcaaaag cctggcctgt tgcaatcttt ccgcgcaatt gccggtaacc
    12181 gcccactgtt cattctgtgt attgccaacc tctgcacctt aggggcgttt aacgtcaagc
    12241 tcgccattca ggtctattac acccagtacg tacttaacga tcccatcctg ttgtcgtata
    12301 tgggattttt cagcatgggc tgtattttca tcggcgtgtt cctgatgcct ggcgcagtca
    12361 gacgttttgg taagaagaag gtctatatcg gcggcctgct gatttgggtg ctgggcgatc
    12421 tgctcaacta tttcttcggc ggcggctcgg tcagctttgt ggcgttctcc tgcctggcgt
    12481 tcttcggctc agcgcctgaa ctgggcgctg gtttccgaca ccgtcgagta cggcgagtgg
    12541 cgcaccggcg tgcgttcgga aggcacggtc tacaccggct tcaccttctt tcgcaaagtg
    12601 tctcaggcgc tggctggttt cttccccggc tggatgctga cgcaaattgg ctatgtgccg
    12661 aacgtcgcac aggctgacca caccattgaa gggttacgcc agttgatctt catctaccca
    12721 agcgcactgg cggtagtcac cattgtggcg atgggttgct tctacagcct gaacgagaag
    12781 atgtatgtcc gcattgtgga agagatagaa gcccgtaaac gcacggcgta ataataataa
    12841 acgacgccct gcggggcgtt ataaggagtg attatgtctg accataatcc actgacatta
    12901 aaactgaatc tgcgggaaaa aatcgcctat ggtatgggcg acgtcggttc gaatttaatg
    12961 ctctgcatcg gtactctgta tctcctcaaa ctttataccg atgaattagg gatgcctgct
    13021 tactatggcg ggattatctt tctggtcgcg aagtttttta ccgcgtttac cgatatgctc
    13081 accggatttt tactcgactc gcggaaaaat attgggccta aaggcaaatt tcgtcccttt
    13141 attttatatg cagcagttcc ggcggcgtta attgcaacgc tccagtttat cgccaccacc
    13201 ttttgtttgc cggttaaaac gacgattgcc accacgctgt ttatgatgtt tggactttca
    13261 tacagtctga tgaactgctc gtatggtgcg atgatcccgg caattaccaa aaacccgaat
    13321 gagcgcgcgc agctcgcggc ttaccgccag ggtggtgcaa ccatagggct attgatttgt
    13381 accgtggcgt ttattccgtt gcagtcgctt ttttctgact caaccgtcgg ttatgcctgt
    13441 gcggcactta tgttctccat tggcggcttt atttttatga tgctgtgcta cagaggcgtc
    13501 aaagagcatt atgtggacac agcgccaacc ggacataaag ccagtattct caaatctttt
    13561 tgcgcgatat ttcgtaatcc accattgctg gttttatgca ttgctaacct gtgtactctg
    13621 gcggcattta atatcaaact ggcgattcag gtctattaca cccagtatgt gctgaatgat
    13681 attaatttat tgtcgtggat gggattcttc agtatgggat gcatcctcgt cggcgtatta
    13741 ctggtgccag taactgtaaa atgttttggt aaaaaacagg tttatttagc tggcatggtg
    13801 ctatgggcgg tgggtgatat actgaattat ttctggggaa gtaactcttt cactttcgtc
    13861 atgttctctt gtgtcgcctt ttttggcacg gcctttgtaa acagcctgaa ctgggcactg
    13921 gtaccagata ccgtagatta cggtgaatgg aaaaccggta ttcgtgccga aggttctgtt
    13981 tataccggtt ataccttctt tcgtaaaatt tctgccgcac ttgctggctt cttgccaggc
    14041 attatgctga cgcaaattgg ttatgttccc aacatcgcgc aaagcgatgc gacattacag
    14101 ggtttgcgtc agctcatttt tatctggcct tgtgcactgg caattattgc tgcattgacg
    14161 atgggattct tttacacact caatgaaaaa cggtttgcat tgattattga ggaaatcaac
    14221 caacgcaaaa ataaagagat ggcgacagaa aaaaaaacgg cttccgtaac gttataaatg
    14281 tcaatagccg ctatttccat cctggtgggt ggcggcctcc ctacgtttaa aaaatggact
    14341 tattatgaaa aagattaatg caataatttt attatcttct ctgacctctg cctcggtatt
    14401 tgctggcg
//
LOCUS       DAJTQQ010000092        14253 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000092 DAJTQQ010000000
VERSION     DAJTQQ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.092"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..106)
                     /locus_tag="OO309_003156"
     CDS             complement(<1..106)
                     /locus_tag="OO309_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCR5848209.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(147..602)
                     /gene="csgB"
                     /locus_tag="OO309_003157"
     CDS             complement(147..602)
                     /gene="csgB"
                     /locus_tag="OO309_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR5848210.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
     gene            1356..2006
                     /gene="csgD"
                     /locus_tag="OO309_003158"
     CDS             1356..2006
                     /gene="csgD"
                     /locus_tag="OO309_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR5848211.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2011..2400
                     /gene="csgE"
                     /locus_tag="OO309_003159"
     CDS             2011..2400
                     /gene="csgE"
                     /locus_tag="OO309_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR5848212.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            2425..2841
                     /gene="csgF"
                     /locus_tag="OO309_003160"
     CDS             2425..2841
                     /gene="csgF"
                     /locus_tag="OO309_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR5848213.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2868..3574
                     /locus_tag="OO309_003161"
                     /pseudo
     CDS             2868..3574
                     /locus_tag="OO309_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3707..4198)
                     /locus_tag="OO309_003162"
     CDS             complement(3707..4198)
                     /locus_tag="OO309_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR5848214.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4300..4854)
                     /gene="ycdY"
                     /locus_tag="OO309_003163"
     CDS             complement(4300..4854)
                     /gene="ycdY"
                     /locus_tag="OO309_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR5848215.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4878..5615)
                     /gene="ycdX"
                     /locus_tag="OO309_003164"
     CDS             complement(4878..5615)
                     /gene="ycdX"
                     /locus_tag="OO309_003164"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR5848216.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5670..6608)
                     /gene="ghrA"
                     /locus_tag="OO309_003165"
                     /pseudo
     CDS             complement(5670..6608)
                     /gene="ghrA"
                     /locus_tag="OO309_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6843..6930
                     /locus_tag="OO309_003166"
     tRNA            6843..6930
                     /locus_tag="OO309_003166"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6877..6879,aa:Ser,seq:gga)
     gene            complement(7080..7361)
                     /locus_tag="OO309_003167"
                     /pseudo
     CDS             complement(7080..7361)
                     /locus_tag="OO309_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7643..8647)
                     /locus_tag="OO309_003168"
     CDS             complement(7643..8647)
                     /locus_tag="OO309_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCR5848217.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(8719..9279)
                     /locus_tag="OO309_003169"
                     /pseudo
     CDS             complement(8719..9279)
                     /locus_tag="OO309_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(9364..9561)
                     /locus_tag="OO309_003170"
     CDS             complement(9364..9561)
                     /locus_tag="OO309_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848218.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(9573..10064)
                     /locus_tag="OO309_003171"
     CDS             complement(9573..10064)
                     /locus_tag="OO309_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848219.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(10061..10435)
                     /locus_tag="OO309_003172"
     CDS             complement(10061..10435)
                     /locus_tag="OO309_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR5848220.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(10482..10856)
                     /locus_tag="OO309_003173"
     CDS             complement(10482..10856)
                     /locus_tag="OO309_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR5848221.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(11019..11240)
                     /locus_tag="OO309_003174"
     CDS             complement(11019..11240)
                     /locus_tag="OO309_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR5848222.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(11303..11779)
                     /locus_tag="OO309_003175"
     CDS             complement(11303..11779)
                     /locus_tag="OO309_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR5848223.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(11795..12268)
                     /locus_tag="OO309_003176"
     CDS             complement(11795..12268)
                     /locus_tag="OO309_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR5848224.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(12610..13431)
                     /locus_tag="OO309_003177"
     CDS             complement(12610..13431)
                     /locus_tag="OO309_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR5848225.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(13531..13704)
                     /locus_tag="OO309_003178"
     CDS             complement(13531..13704)
                     /locus_tag="OO309_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR5848226.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            complement(13824..>14253)
                     /locus_tag="OO309_003179"
     CDS             complement(13824..>14253)
                     /locus_tag="OO309_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848227.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
BASE COUNT         3642 a         3493 c         3412 g         3706 t
ORIGIN      
        1 cgccaccacc gtggttaccg ccgccgccgt actgaggaac aacacctgcc agagcgctac
       61 cggagaatac gattgctgca attgctgcta cttttaaaag tttcatgtaa aacccccatc
      121 ggattgattt aaaagtcgaa tggtaattaa cgttgtgtca cgcgaattgc catttgcgac
      181 tgtctctgca ctacaactgc cgttttttga gtaccatact gtgtaatatt tgctttatta
      241 ccagaacctt tctggataat catcgcagta ttaccataag caccttgcga aatactggca
      301 tcgttggcac tgcccgcctg atcaatatat gcaaggttat aatctcctgt ctggtcaatc
      361 tttgcccgat tgctactacc ttcttgcgca acaaccgcca aaagttttga gcctccctgc
      421 cgtaactgag cactattatt agtcccagcc tgaccaatta tggctgcctg attaaatgaa
      481 gacttactca attcatttac cgcgaagtta tattctgaat tagctaaatc ataacctgct
      541 gcggctgcaa tcccaggcgc acccagtatt gttaacatca taaataacaa tttgtttttc
      601 atgttgtcac cctggacctg gtcgtacatt taagaaatta aatcatttca acttggttgt
      661 taacgcaacc tgtattttgt taacgctgcg ttacgatgga aagtatgtct gcggaaatat
      721 ttttaataac tcacccgcgc gttgtatttt tctttttttg ttcataccaa agtattaaaa
      781 cccgcgtaaa tacgctgtta tctacgcaaa aaatattttg ttttctctta aatctccgtt
      841 ttccgctatc aaaaatcacc agacagtcat tcttcttgcc cgtcgctgat tgctgcgata
      901 tgtcttgcgc acaagccgtg acaaacctcg tctttttgca gtaaaaaaat tgtccacgga
      961 ggtgtggaga aaaaacaaaa acgttttaca tgacgaaagg actacaccga aatatttttt
     1021 atatgtatta ttagtaagtt agcaccattt gtatgatttt ttaaaattgt gcaataaaaa
     1081 ccaaatgtac aacttttcta tcatttctaa acttaataaa accttaatgt taacatttta
     1141 atataatgag ttacattttg ttacatgttt aacacttgat ttaagatttg taatggctag
     1201 attgaaatca gatgtaatcc attagtttta tattttaccc atttagggct gatttattac
     1261 tacacacagc agtgcaacat ctgtcagtac ttctggtgct tctattttag aggcagctgt
     1321 caggtgtgcg atcaataaaa aagtggggtt tcatcatgtt taatgaagtc catagtattc
     1381 atggtcatac attattgttg atcactaaac cttctttgca ggcgacagct ctcttgcagc
     1441 accttaaaca atcgctagca attacaggaa aattacataa tattcaacgt tctctggacg
     1501 atatctcttc aggctctatt attcttctgg atatgatgga agcggataaa aagcttatcc
     1561 attattggca ggatactttg agcaggaaaa acaacaatat caaaatattg ttgctaaata
     1621 cgcctgaaga ttacccgtac cgcgacattg aaaactggcc tcatatcaac ggcgtttttt
     1681 atgccatgga ggatcaagaa cgtgttgtca atgggttgca aggcgtcctg cgtggcgaat
     1741 gctactttac gcaaaagctt gccagttacc tgattacaca ttctggtaac tatcgttata
     1801 acagcacgga atcagccctc cttactcatc gggaaaaaga gatcctgaat aagctgcgta
     1861 tcggcgcgtc taataacgag atcgctcgtt cgttgttcat cagcgaaaat acggttaaaa
     1921 cgcatcttta taatcttttc aagaagatag ccgtaaaaaa ccggacacaa gcggtttcct
     1981 gggcaaacga taacctcagg cgataaagcc atgaaacgtt atttacgctg gattgtggcg
     2041 gcagaatttc tgttcgccgc agggaatctt cacgccgttg aggtagaagt cccgggattg
     2101 ttaactgacc atactgtttc atctattggc catgattttt accgagcctt tagtgataaa
     2161 tgggaaagtg actatacggg taacttaacg attaatgaaa ggcccagtgc acgatgggga
     2221 agctggatca caataacggt caatcaggat gttattttcc agactttttt attcccgttg
     2281 aaaagagact ccgagaaaac tgtcgtcttt gcactgattc aaactgaaga agcactaaat
     2341 cgtcgccaga taaatcaggc gttattaagt acggacgatt tggcgcatga tgaattctaa
     2401 ataaaaaatt gttcggaggc tgcaatgcgt gtcaaacatg cagtagttct actcatgctt
     2461 atttcgccat taagttgggc tggaaccatg actttccagt tccgtaatcc aaactttggt
     2521 ggtaacccaa ataatggcgc ttttttatta aatagcgctc aggcccaaaa ctcttataaa
     2581 gatccgagct ataacgatga ctttggtatt gaaacaccct cagcgttaga taactttact
     2641 caggccatcc agtcacaaat tttaggtggg ctactgtcga atattaatac cggtaaaccg
     2701 ggccgcatgg tgaccaacga ttatattgtt gatattgcta accgcgatgg tcaattgcag
     2761 ttgaacgtga cagatcgtaa aaccggacaa acctcgacca tccaggtttc gggtttacaa
     2821 aataactcaa ccgattttta agccccagct tcataaggaa aataatcatg cagcgcttat
     2881 ttcttttggt tgccgtcatg ttactgagcg gatgcttaac cgccccgcct aaagaagccg
     2941 ccagaccgac attaatgcct cgtgctcaga gctacaaaga tttgacccat ctgccagcgc
     3001 cgacgggtaa gatctttgtt tcggtataca acattcagga cgaaaccggg caatttaaac
     3061 cctacccggc aagtaacttc tccactgctg ttccgcaaag cgccagggct tacaaaacct
     3121 gcttaacgag cgcaaggtta ttcgtgcggc acaagaaaac ggcacggttg ccattaataa
     3181 ccgaatcccg ctgcaatctt taacggcggc aaatattatg gttgaaggtt cgattatcgg
     3241 ttatgaaagc aacgttaaat cgggcggtgt tggggcaaga tattttggga tcggtgcaga
     3301 cacgcaatac cagctcgatc agattgccgt gaacctgcgt gtcgtcaatg tgagtaccgg
     3361 tgagatcctc tcctcggtga acaccagtaa gacgatactt tcctatgaag ttcaggccgg
     3421 ggttttccgc tttattgact accagcgctt gctggaaggg gaagtgggtt acacctcaaa
     3481 cgaacccgtt atgctgtgtc tgatgtcggc tatcgaaaca ggggtcattt tcctgattaa
     3541 tgatggtatc gaccgtggtc tgtgggattt gtaaaataaa gcagaacggc agaatgacat
     3601 tctggtgaaa taccgccata tgtcggttcc accggaatcc tgacagataa aaatgccgga
     3661 attaataatc cggcatttac tgtttaagcg aaagcgtgag tgcccctcac gctttatttt
     3721 tgatttcctg ctcacggtga gccgtctttg cactacgcgc cgccagccac aggccgctgt
     3781 ttttcattgc gtaaccaaat accagcccaa gcgccagcga aggtaacacc agtttccagt
     3841 caccctgccc ggcaaacgtc gcgcatgcgc ctataaacgt ccccggaaca aatgaaagca
     3901 gcagctgttt ggcctgaata cacatcagaa acgcgacaat accggttatg acataaccga
     3961 gaatttccag atgtggtgcc agtgcactac cgtaaataat gaccatcgcc cacaccacgc
     4021 cactaagcag ggttgcagcg gagatcgcca gccctttcag cccaccttgc gggcaagcaa
     4081 agtaggccgt acagcccagg aagcccgccc agcttagtaa gcccagggaa acagccaccc
     4141 atccccagat accggagaga atgcctgttg tgattgcaat agagagaagt atgttcatgc
     4201 cgcgcatgat agcagaaaac gcggccatga atgagatcga gcacacattt tatgcaacaa
     4261 caaattatct gttgcagtta tgagtgacga tgatcacatt tactcttcag aatcttcttc
     4321 cagctcgtcc cacattgcac taatggcatc acgggttagc ggtgccatgg tgcgccagaa
     4381 aggcgtggtt gcatgggcct ccactttgcc gaggaacgca ccacaccagg gtaacagata
     4441 ctcactgaac agtgtttcca gtgcttcgct ctcatcttcc gttgactgat cttccagcca
     4501 ggaagccgca agcagcaatg tgccgatgtg atcggctggc gtatccgcca gtagcatccc
     4561 tcgttcggaa agaaaagcgc gcacttccgc ttccgttgcg tcctcaaccc atgcgctacg
     4621 atatggcggc acggcacatt catcgccgat aaacaacgca ttgtaatcgg cagagacttg
     4681 cgtcatatca cagcttttct gtaaacgcgt cagtaactca tcctgctcca gtggccagtt
     4741 cgcagccagt ttcccctcac gaatcagagt aaacagcggc accagtaaag gatcttgcgg
     4801 ttggcggtaa tagagcgaac ccagcacacg acagaggata gaaaactcgt tcattaatat
     4861 atttccatta ctagtaatta aaggtctgca aattccgcaa tcggtgccat accgcgagat
     4921 tcaaggaagt tcagtaagcg gcgcggagaa acattcaaaa tgcgctctag cggaaaatct
     4981 accgcgtcga ggattttaag acactcttca aattccccca tggtaaacgc ggtgtgagaa
     5041 tccgagccta atgccaccca acctccagca tcacgtaccg ctgcagctac cgcacgacag
     5101 ttgtcttcac tgcccttacg tgagtgtaaa aatgaggaat tattgatttc cagcgccacc
     5161 tgatgtttcg cggctgcttc agcaacggct ttaacatcta tttcatattt cggatttccg
     5221 ggatggctta ttatatgcac attgccgctg gcgatagtgg agatcatcgc ttgtgtattg
     5281 gtcgctttgt catgtggcgc aaaaaccggc tcatgaaaac cggcaataat cagatccagc
     5341 gagtcaaaca ttttaccgct gcagtcaatt tcaccatcaa catttttaat gttagcttca
     5401 atgccgcgca ggatccctac cccatcaacc actcgcggcc agatacgcat gttaatgaag
     5461 tgccagtgat gcggcgcatc ttccatatcc gggccatgat cggtgatcgc aaaaagttta
     5521 atgccctttt gtttggcctg ggcaatgtaa tcactcaatg tgctatatgc atgtgtgctg
     5581 gcaacggtat gcatatgaag gtcgacggga tacataactc tctcctgtat tcaattcccg
     5641 ccaaggatag caggaatcct gatgctttat tagtagccac gtgcgcggtc gacttgcccg
     5701 cagaccctct cccctttttc gagctgggca atagtgcgag aaatgtactc cacagcttca
     5761 gcgggacggg taatcgcggc gacatgtggt gttatcgtca cgcgtggatg ttgccagagc
     5821 ggactttcag gcggtaaggg ttcacgatta aaaacatcca gcattgcgcc tttaactttc
     5881 ccgctatcca gcgccgcgag caggtcatct tccacaacat gaacaccacg cgccaggttg
     5941 aggagatacg cgccatcagg taatttttcg agtaattgtt gattaataat gccgacggtt
     6001 tcaggggtat tcggtaacaa attaatcaat acccgacatt ggctcagaaa tgcagacagt
     6061 tcttcccgtc cggcaaagct ttgcacgcca ggccacgatt tacgggttcg actccagcaa
     6121 cgcagcggaa agcgctaggt ttgcagactc tgagcaactt tacagcccag tacgcctgcg
     6181 cccaaaatgc cgatggtaaa atcttcccga tgatattcag gcagcggttg ccaatgcgaa
     6241 ctattttgct ggatgcgata atcgtcaaaa cgtcgaaacc aatgcagcac ctgactgaca
     6301 gcatattcct gcatttgctc gcccataccg gtatcttcca ggcgaaaaag tggaacagaa
     6361 gggttcagca tttcagggtg tgcctgtagc ttgctcaaaa tagaatcaac accggccccg
     6421 agtgcgaaca ccgctttaag atcgcgccct gccagcattt caacaggagg atgccagact
     6481 aaaacataat cagcagagtc attatctccg cttttccatg ccctgactct tgcctgagga
     6541 atagctttgc gcagtgcctc aatccaccat tgggtatcga acgttgggtg ataaaagatg
     6601 atatccatac tgactcccga aaagcgttgt gcgaaattta ttcgcactta tcgttatgat
     6661 ctacaaaggc caccagcata acaaatccgt ggtcggtggc aaaaaaagca gatttcgctt
     6721 attaaaacca cacattgatt gaatgtttga ataaacgcgc gattttttca aaaagtttgt
     6781 tgacctcagg tcatgatttc cctaaattag cgcccgttcc agcaagacag gaacgacaat
     6841 ttggtgaggt gtccgagtgg ctgaaggagc acgcctggaa agtgtgtata cggcaacgta
     6901 tcgggggttc gaatcccccc ctcaccgcca gattataagt gcatgttatt gtttaacatg
     6961 cgctttttta tttttccttc cagacagtat gccatcgggt atgccatctg aaaccggttt
     7021 atatgccccg cgatcatatc ttatcggaca atttccgatc tttgagcata ctggcaaagc
     7081 tatactgcca gcatatcagg atagtgtttt gcgataatat cattcagtct gtcaaccagt
     7141 tctggttttc tgaacgtgat atgcgcggag cccttcttaa agtatctgat gctgaacatc
     7201 tcatcttcat agctctcttt accctgaaca gactgaatgt gatcatccag acgaagggtg
     7261 atgtttcccc ggttgtcggg gattggttta ccgctgaaca ggtgcaacat tctttccaga
     7321 tcggcgagtc ggtcagcctg ttgtccggtg ataagatgaa atctatggtc acccccgttt
     7381 ttgcaacact gatttttgat taatggttgg cttgcttaaa tctatccggc gtctctgtgg
     7441 gcaaggatgc ccgcgccccg atgagttccg cacctgataa tcctaaaaat cccctcggct
     7501 tcagtgagcc atttcatctg ttaggttttt aatcaggtcg atttgccgtt catgtcatca
     7561 ataatcagtc ttcgcaaaac cagaaaggta acttgtatct tattgtttaa aataaactaa
     7621 ttgagttata tcagcatcta tatcaggcct gatattcttt tttctgagta gtgattgccc
     7681 aggcaattcg agccagttta tttgccagag cgcaggtaac cacattcgaa tgcttcttgt
     7741 tgagttgctc ccgaacccac tcagcaagtt ttccatgctt gcgttcaagt cgcattatga
     7801 atgagcgggc acactgaacc agtaaccggc gtaaattttt atcacctcgc ttactgatac
     7861 ccaacagagt cgattttcct ccagtgctat actgccgggg aacaagtcct gtggaagctg
     7921 caaaatccct gctgcatgag aactgtttcc cgtcaccaag ttgcgacgat agtaagctgg
     7981 cagtaatcgg accaacacca ggaattgtca taatgcgctg agcggtatca tcagcgttga
     8041 ttgactgact taattctgat tccagctctg ctatttgctc aacaaggtaa aagtagtggg
     8101 tatgcaacct caccaaaagg cggctcagat attcggggat ttcgtgttca gccaacacga
     8161 gagacaaccg ttttattaca gcatcaccta tcggcaggct gattccaaat tccagcaaga
     8221 aaccgtggat ctggttggtt gttttcacct tatctctgat gagcgattct ctgacccggt
     8281 gtaacgccct catggcctgc tgggtctcag ttcttggctg gacaaaacgc attgatggtc
     8341 gtgaagccgc ttcgcatatg gcttctgcat caacaaaatc attcttatta cttttgacaa
     8401 acgggcgaac gaactgtgga gaaatcagct tagtttcatg cccaaaatca gagatacagc
     8461 gagccataaa gtgtgcaccc gcacaggctt ccattacaac aactgagggc ggacatgtcg
     8521 ccagaaactg catcagttta gtacgggtaa attttttacg cagaagagcc ttaccagact
     8581 tgtcttgaca atgaatgtgg aaggagtgct ttccgagatc gataccaata agtgtgatgt
     8641 tctgcatgat ggtctctccg ttaaagggat ttaccttaac agcgtactgc tgttaaggtg
     8701 ggggtgacca tatcattatc cccaccggtc ccatctcacc agattgttga cgataatttt
     8761 actgccaaat ttacaggggc aattggtttt gtaattccag ttcagccctc tgaagacgtt
     8821 gatcacccct cgctcaaaca cctcatcctt attctggtgc agttgttcaa atgtgctcag
     8881 aatgttcgct tcgctgattt ccggaaaatt atcgtactcc agtgaccggt accatgtatc
     8941 acgggcctgc gtatccatta atgacagcat cccggaacgt tgcatcaggt cacgccagat
     9001 ttcgcgatca agattccggg taatggcttt cattgctgtt tcgggtttct ccatcagcca
     9061 gcagccgcag cgaaaatcct gtttcatggc ccagtccggg gcagttttac ctccgatgct
     9121 ttccgtcagc accgagattt cagccagttg tcggataagt gtgtcgattt gcaacagtgt
     9181 actctttcgt acagccagaa tgtggcgaat gctggttgag cagataacgt ccgtatgccc
     9241 gttgaccact tccggacggg agattgtgat atctgacata atcgatattc ctttttgcag
     9301 ataaatgaaa acgccagccc tgtgcaggct ggcgtcagag gaatgaagtc tggtgagtga
     9361 gcttcaccgg ttctggtgca gaagctgcag atgacgcagg gtacggacat cctcccgggc
     9421 ctgttcgata cagggcaaca gtgctgctga atccgtttta tcctcatcgt tgtcaaagat
     9481 aattcccgat ccgcagtcga tattgtcctg cagccacgca atcagaatat ccagcgctgt
     9541 ttccgtggtt aatgatttca tgttgttcct ggttaccagt cgaaagtggg taaacccggc
     9601 aggcattcag cgctggcatc cagatgaatg agactgacgc cataacgccg gatgagtgtg
     9661 acgaccagac gacggaacgt ttttgacagc cccaggcgtt taagccgcag aaccgggtat
     9721 gaccacgcat cggtacgtaa cagatatccg ctacctgtaa aatgaatcca ttctgattca
     9781 ccgaagtcac tggtctggtg tgacagcgag tacaaccagg cgttgtccgt ttccgtgata
     9841 tgtgcggtac tgcagcgtat gccggtaagg gtagcaaatg gtggcgtgca ttctgctgag
     9901 gtctggtcag attcatcaac aacacgaagg gagtagccgt tatcgcagac catgttaatc
     9961 agttcagcga gctcaatacc atcgacgtca acgacaatgc gccctatgtt cagtgcctgt
    10021 acgttaacgc tgtcggcttc cagtgtcagg gccagtttca tcatttcgcc tccggatact
    10081 tacccagggt aatgttattt accgttctgt aattgtcgcg ggtcatcagg ccggtcgccc
    10141 ggcgagcccg gaggatatca atactgttta ttaactgaga gcggggacag gtgctgaatc
    10201 ccggctggtc ggtacgcacc agcgcgtatt tttccacgag aaaattaact gcatcacaca
    10261 gtgaaatgcc tgcctcaata tgctgctcaa tcacacgttc atcgacaaac ggtgtgtcat
    10321 tcagtgtgag gccataatgc tggtccagca gtcggatgag cagtgtctgc cagatggtga
    10381 cgggagacgg gcagcgagat acctcccgta catgtgtgtc ggataatgtt ttcatcttaa
    10441 gttctctgta actggtagtg ctaaaggtga gagcttcggg gttatgaggt ggttgcgggt
    10501 gctggtgtcg gataaacagc cagataaacg taaccacagg agccgagggt gtcggcttcg
    10561 caggtcagcc cttttgcata cagcgtgacg gtatgctgat atcggggatt cagttcaccg
    10621 ctggtgagca tgagttccag ttgtttcatc agcagcggaa aggcctggtc cagatggtat
    10681 gcatctgcgt cgctgaagcg gcctctgata ccggcgcggt cggcaaggta atacaggcgg
    10741 ttaccctctt gcaccagacg agcgccaaaa cagggcctca ctgtgcaggg aagcccccac
    10801 caggggcggt cgtgattgtc atcgggatga gttatcccgg agagtttgtc tgacacgata
    10861 aaatcctctc tgaaaagtgg ttaagaatgg tttaacgatg acaggtcagc cggcgcgctg
    10921 accggtttcg gtgaagatgt atgcattgat gagaatggcc tcatccacag cggcgtctga
    10981 ggtcatctgg tcgtactcgt ttcccgggtg acggaacatc agttcagggt gacactcatc
    11041 agccggacat acgggccaaa actgtcctta cggcgttctg caaacacggc aagaacgccg
    11101 ggaatatcct gcacttcacg accagtatac gcttcagcac tgccgtgcca gcgatactta
    11161 ccggtacaga acggaaaaag acgggatgca ggatgctgtt ggtgaatacg catggcttca
    11221 ccacgggtga tgattctcat gatgtgatac ctctgaagac agaagataaa agtgaaaaca
    11281 ggtgtgatgt ggttgtgacg ggttaaagca gaccatgttc ggcaaaggag aaaacctggt
    11341 tgccaccgac tatcagatgg tccggcaccc ggatatccac caggcccagt gcctgtacca
    11401 gacgttcggt gataagccgg tctgccttac tgggtgtgac ttcaccggac gggtgattgt
    11461 gcgccagcac cacggcagcg gcattgtggt acagggcgcg tttaatcact tcccggggat
    11521 gaacttccgt acggttgatg gtgccggtga agagggtttc accggcaatc agctgattct
    11581 ggttgttcag atacagcacc cggaactctt cccgctccag tcccgccatg ttcagaatca
    11641 gccattcccg tgccgcacgg gtggaggtga aggccacgcc gggttcatga agatggcggt
    11701 ccagggtttg cagggcccgc agaatgagac tgcgctcgcc gggcgtcatg tttccgggca
    11761 gaaaggaaag ctgctgcatt gtccctgcct ccattcagtc gatgatgcgc ataatggcgc
    11821 tgcattccgg atgctgcagg gcgtaatccc gcagccggta ataatggacc gtcatggcat
    11881 aacactccgt acgacaggca tgatgactgt acgtcatcag acaggcagca atgccggcgg
    11941 cttccgggct catttcagcg cggttaccgt tcatggcatt gaacagtacc catgtttcgt
    12001 catcatcgtc atccggttcg ggtgccataa atgccccgcc gttgttcagg gtgtacagat
    12061 tccagatacc accgcagtag tcttcacaca gacggtccat ccagccgaag acacggggtt
    12121 ccagggtgac ccactgtgga atgaggccaa aatgctgcgg ccagaagctg atgcgctgtt
    12181 catcagggac gggagtggca accagttgtt gcaggccagt ctccggtgca caggccgaag
    12241 cggtggtgtt ctgagacaat gttttcatgt atgaaatcct tgtaatgtga ataaatgaga
    12301 gggagtaaag gtcgggagct ggcaacatca cgttcatcac tgaccgtgat gacccggtaa
    12361 tatcagggat gaatgatatt cggctgaaat tccggacagc cgtacgcgta accgttccgg
    12421 tcacagacgg atggccagat gccattgcct gtatactcag tctgcacaga ctgatttctc
    12481 cgggacggta aagcagacgg gtacaccagg cgcacaggtc atcatcagaa atggaacgac
    12541 accccggcag cgcctccggg gcgtcgggtg attctggtgc ctgaatcacg ccttcaggga
    12601 catgctgcct cagcgcaggc tctccagcag cgtttctgcc attacccaca atgcgcggtt
    12661 gagcttaata tcggtgtcga tgttgtgaat ggcacgggtg tggatacgtt ttccttttgc
    12721 actgcggccg gaaatcccgc ctttcagcat attctcctgg atggtctgat atgcgctcca
    12781 caggtcttta ccgtaatcct ccctgcgtcg tggtgtcaga atgtcagcgg tggtgactgg
    12841 ctgatgttcg tcaccataac ggtaagtcag tgccgcctgt gccagcgcct ggcgtgccgg
    12901 tggcggcagg accagcgact gcatggcatc acgcttctcc tctatccggt caaacacgcc
    12961 caccacctcg taagcccctt caataacttt ctccaccaca tttccccggt gtggaacacg
    13021 caattctccc agagactgac cgcagaccag tccattggta caaatggcac gaaaatatcc
    13081 gggtaacatc tggtagctgg atgaaccgtc atgagagttg agcagaataa tttcagggac
    13141 atgctgaccg gttatctgtc cggcccgccg cagacgcagc atgtgttttg tgtattcccg
    13201 gcgacccggg tcacgcacac gtgtctggca ggcgaagaat ggctgaaagc cttcccgctg
    13261 caggctttcc agtacggtga tggtgggagt gtacgcatag cgtttactgc gggaagtatg
    13321 ccggtcttca ccgaaaatac tgggaacata gtgcatcagt tcttcgtgtg tcagcggacg
    13381 gtcacggcgt atctggtttg cagcataacc aaaacgactg gctagtcgca taatttgctc
    13441 cttatcggtg gttaagattt actggtgtaa taaatgaaaa agccacgtct cccggagaag
    13501 acgcggcctg atggacgaaa ttaatgaggt ttattgtctg agaagaccgt cactggcgaa
    13561 ccttccggcg tcagtctctg cgttgcggat aacccgacgg aaatgagttc cctggtcatc
    13621 gtcgataaac acattgcggt aagctgttgt gatgacaaca gcctgctcgc gtgagaaagg
    13681 gacttcgggc aataaacgtg acatacaacc agacctgtat ttggttccct gaaaatataa
    13741 gtccctgtca tcatggcaga gccgtattta cttttgatat caataaatta ataacgcgaa
    13801 gatgaaccgc ataacttacg cctttaattt gttttgttta cgtagtccct gaccaggtac
    13861 gcgaattcgt gaacgccagt ttcaccaaac caaatcaggt aaccagggtc gatgttgtaa
    13921 tagtaaccaa cgactttatg cgtttttggg ttatagagtg ttacgatata gtcccagccg
    13981 caggcgtggg ctttacaacc gccaccttct acccaggtcg ttccgttaat gtttacagga
    14041 tacgcaccac catcaggacc atccatatag ccgtcttccc caaacattcc gtttaaccca
    14101 acagggttta atgctttgtc gatagcagct ctgtaggtag cgtcaccttc gtataactcg
    14161 tacgttgttg gacatgcacc aggctggtca gcccgggctt ttgagtcgca tttaagtgtc
    14221 tgatttacag tggcggcgtt tgtgtcagga ctt
//
LOCUS       DAJTQQ010000093        14175 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000093 DAJTQQ010000000
VERSION     DAJTQQ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14175
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.093"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..75)
                     /locus_tag="OO309_003180"
     CDS             complement(<1..75)
                     /locus_tag="OO309_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR5848228.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQA"
     gene            349..3873
                     /gene="nifJ"
                     /locus_tag="OO309_003181"
     CDS             349..3873
                     /gene="nifJ"
                     /locus_tag="OO309_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR5848229.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4240..5369
                     /gene="ompN"
                     /locus_tag="OO309_003182"
                     /pseudo
     CDS             4240..5369
                     /gene="ompN"
                     /locus_tag="OO309_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5510..5944
                     /gene="uspF"
                     /locus_tag="OO309_003183"
     CDS             5510..5944
                     /gene="uspF"
                     /locus_tag="OO309_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR5848230.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6120..7055
                     /gene="ttcA"
                     /locus_tag="OO309_003184"
     CDS             6120..7055
                     /gene="ttcA"
                     /locus_tag="OO309_003184"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR5848231.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7184..8551)
                     /gene="dbpA"
                     /locus_tag="OO309_003185"
     CDS             complement(7184..8551)
                     /gene="dbpA"
                     /locus_tag="OO309_003185"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR5848232.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9029..10012)
                     /gene="zntB"
                     /locus_tag="OO309_003186"
     CDS             complement(9029..10012)
                     /gene="zntB"
                     /locus_tag="OO309_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR5848233.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10267..11499
                     /gene="dgcM"
                     /locus_tag="OO309_003187"
                     /pseudo
     CDS             10267..11499
                     /gene="dgcM"
                     /locus_tag="OO309_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11520..12083)
                     /gene="smrA"
                     /locus_tag="OO309_003188"
                     /pseudo
     CDS             complement(11520..12083)
                     /gene="smrA"
                     /locus_tag="OO309_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12337..13791)
                     /locus_tag="OO309_003189"
     CDS             complement(12337..13791)
                     /locus_tag="OO309_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCR5848234.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(13788..>14175)
                     /locus_tag="OO309_003190"
     CDS             complement(13788..>14175)
                     /locus_tag="OO309_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5848235.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3777 a         3568 c         3345 g         3485 t
ORIGIN      
        1 cgcctgctga acaggctcac tactacacgc cgacaacaat aaagcggcac accctaccca
       61 aaacgctgct cgcatctctt cttcctctga tcttcaagcc aaacgacacc gccataaata
      121 ataggcagca cagagggcgg cgtcgagagc tgtcctgcgc gttgccccgc atttttactt
      181 ttttatggct atttttttgc cctctgtttg atcaaaacat tcattactct gatgtggggg
      241 acacaaaagc gaaaatgcag aagaaagcca tttgctaaaa ttgaaggatt actactgggc
      301 gcgcagcaat ttcgtgcgcc cctcatttgc gcaatgtaag ggtgtcatat gattactatt
      361 gacggtaatg gcgcggttgc ttcggtgacg tttcgcatca gtgaagttat cgccatctac
      421 cctattaccc ccagttccac gatggcagaa caggctgatg cctgggccgg aaacggcttg
      481 aagaacgttt ggggagacac accacgcgtg gttgaaatgc agtcggaagc gggtgctatc
      541 gctaccgtgc atggcgcttt gcagacgggt gccctttcaa catcgtttac gtcatcgcag
      601 ggtttgctgc tgatgatccc gacgctgtac aaactggcag gcgaactgac gccatttgtc
      661 ctgcatgtag cggcacgtac cgttgccaca catgcacttt ctatttttgg cgatcattcc
      721 gacgttatgg cggtgcgcca gacgggttgc gcgatgttgt gtgcggctaa cgtccaggaa
      781 gcgcaagact ttgctctcat ttcgcacatc gcaacgctga agagccgcgt gccatttatt
      841 catttctttg atggttttcg cacgtcccac gaaatcaata aaattgtccc gctggccgat
      901 gacactattc ttgatctcat gccgcaggcc gaaattgatg ctcatcgcgc ccgggcgctc
      961 aacccggaac atccggtgat ccgcggtacg tccgccaatc ctgacactta tttccagtct
     1021 cgcgaagcca ccaatccctg gtacaacgcg gtctatgacc atgttgaaca ggcgatgaat
     1081 gatttcgctg ccgcgacagg tcgtcagtat cagccgtttg agtattacgg gcatccgcaa
     1141 gcggaacggg tgattatcct gatgggctct gccattggca cctgtgaaga agtggttgat
     1201 gaattgctaa cccgtggcga aaaagttggc gtgctgaaag ttcgtctgta ccgtcccttc
     1261 tccgccaaac acttgctgca agctctgccg ggatccgtac gcagcgtggc ggtactggac
     1321 agaaccaaag aacccggtgc ccaggcagaa ccgctctatc tggatgtgat gaccgcactg
     1381 gcagaagcct ttaataatgg tgagcgcgaa accctgcccc gtgtcattgg tgggcgctat
     1441 ggtctttcat ccaaagaatt tggcccggac tgtgtactgg cggtatttgc cgagctcaac
     1501 gcggctaaac ctaaagcacg ctttacggtt ggtatttacg atgatgtgac caatctgtca
     1561 ctgccgttgc cggaaaacac tctgccaaac tcggcgaaac ttgaagcctt gttttatggc
     1621 cttggtagtg atggcagcgt ttccgcaacc aaaaacaata tcaagattat cggtaattcc
     1681 acgccgtggt acgcacaggg ctattttgtt tacgactcca aaaaagcggg cggcctgacg
     1741 gtttctcacc ttcgcgtgag cgaacagccg attcgttccg cttatctcat ttcccaggct
     1801 gattttgttg gctgccacca gttgcagttt atcgataaat atcagatggc tgagcgttta
     1861 aaacctagcg gcattttcct gcttaatacg ccgtacagcg cagatgaagt gtggtcgcgc
     1921 ttgccgcagg aagttcaggc cgtgttaaac cagaaaaaag cgcgcttcta tgtgattaac
     1981 gcggcgaaaa tcgcccgcga atgtggcctg gcggcccgta ttaataccgt catgcagatg
     2041 gcttttttcc atctgacgca aattctgcct ggcgatagcg ccctcgcaga attgcagggt
     2101 gcgattgcca aaagttacag tagcaaaggt caggatctgg tggagcgcaa ctggcaggct
     2161 ctggcgctgg cgcgtgaatc cgtagaagaa gttccgttgc aaccggtaaa tccgcacagc
     2221 gccaatcgac cgccagtggt ttccgatgcc gcccctgatt tcgtgaaaac cgtaaccgcg
     2281 gcgatgctcg ccgggcttgg tgatgccctc cccgtttcgg cgctgccgcc agacggcacc
     2341 tggccgatgg gcaccactcg ctgggaaaaa cgcaatatcg ccgaagagat ccccatctgg
     2401 aaagaggaac tctgtaccca atgtaaccac tgcgtcgccg cttgcccaca ctcagctatt
     2461 cgcgcaaaag tggtaccgcc tgaagcgatg gaaaacgccc ctgccagcct gcattcgcta
     2521 gatgtgaaat cgcgtgatat gcgcgggcag aaatatgtct tgcaggtggc accggaagat
     2581 tgcaccggtt gtaacctgtg cgtcgaagtt tgcccggcga aagaccgtca gaatccagag
     2641 attaaagcca tcaatatgat gtctcgccag gaacatgtcg aagaagagaa aatcaattac
     2701 gatttcttcc tcaacctgcc agaaatcgac cgtagcaaac tggaacgtat tgatattcgt
     2761 acatcgcagc tgattacacc gctgtttgaa tattcaggtg cttgccccgg ttgtggcgag
     2821 acgccgtata ttaaattgct gactcagctc tatggtgacc ggatgttgat cgctaacgcc
     2881 accggctgtt cttcaattta tggcggtaac ctgccctcta caccgtatac caccgatgcc
     2941 aacggtcgtg ggccggcatg ggcgaactct ctatttgaag ataatgccga atttggcctt
     3001 ggtttccgcc tgacggtcga tcaacaccgt gtccgcgtgc tgcgtctgct ggatcaattt
     3061 gccgataaaa tcccggcgga attactgacg gcgttgaaat cagacgctac gccagaggtt
     3121 cgtcgagaac aggtcgcagc tttacgccag caactcaacg atgttgccga agcacatgaa
     3181 cttctgcgtg acgcagatgc actggtggaa aaatcaatct ggctgattgg tggcgacggc
     3241 tgggcttacg atatcggctt tggcggtctg gatcatgtat tgagtttgac ggaaaacgtc
     3301 aacattctgg tgctggatac gcaatgctat tccaacaccg gtggtcaggc gtcgaaagcg
     3361 acaccgctgg gtgcagtaac taagttcggc gagcacggca aacgtaaagc gcgtaaagat
     3421 cttggcgtca gtatgatgat gtacggtcat gtttatgtgg cgcagatttc tctcggcgcg
     3481 cagctgaacc agacggtgaa agcgattcag gaagcggaag cgtatccggg accatcgctg
     3541 atcattgctt atagcccgtg tgaagagcat ggttacgatc tggctctcag ccacgaccag
     3601 atgcgccaac tcactgctac cggcttctgg ccgctatatc gctttgatcc gcgtcgtgcc
     3661 gatgaaggca aactgccgct ggcgctggat tcacgcccgc cgtcagaagc actggaagaa
     3721 acgttacttc acgagcagcg tttccgtcgt ctgaattcgc agcagccaga agtggcagaa
     3781 cagttatgga aagatgctgc agctaatttg caaaaacgct atgacttcct agcacaaatg
     3841 gccggaaaag cggaaaaaag caacaccgat taaatgctct ggataaggat tatccaattc
     3901 taaaaaaaaa gcccggacga ctgttcgggc ttgtcttttt atatgttgga aaatcagtgg
     3961 caatgcaatg gcccaacaga aaataaaatc tgtgacaata aaggcatata acccgcgcag
     4021 aataacgtat aggaaattaa aagtataatt tttattttgt atgtattatc ttcgatacat
     4081 tcaccagact tattatcatt tcagaggaat tatttgatta aggttttact taaggcgtaa
     4141 caaatgatat ttatcggcta actgaacttc tcctttagga tgttttcact ccccgaaatg
     4201 gggataaaag gcaaataaaa taactaaagg atttattcaa tgaaaagaaa agtactggca
     4261 cttttaattc ctgccctgct cgccgcaggt gctgcacatg cagccgaagt ttataataaa
     4321 gacggcaaca aattagatct gtatggcaaa gttgatggcc tgcattattt ttctgataat
     4381 tcagcgaaag atggcgacca gagctatgcg cgtctgggtt ttaaaggcga aacccaaatt
     4441 aacgatcaac ttactggcta cggtcaatgg gaatacaata ttcaggcaaa caacactgaa
     4501 tcttcaaaaa accagtcatg gacccgtctg gcatttgccg gactgaaatt tgcagattac
     4561 ggttctttcg attacggacg taattatggc gtaatgtacg acatcgaagg ctggaccgat
     4621 atgctgcctg aatttggcgg tgactcttat accaatgcag acaactttat gactggtcga
     4681 gccaatggcg tcgcgactta tcgtaatact gatttcttcg gtctggtaaa tggtctgaac
     4741 ttcgcggtgc agtatcaagg taacaacgaa ggagccagta atggtcagga aggcaccaat
     4801 aacgaacgcg atgttcgtca tgaaaacggt gacggctggg gtctttccac aacatatgat
     4861 ttaggcatgg gctttagcgc tggtgcggca tacacctctt ctgaccgcac caatgaccag
     4921 gttaaccata ctgcggcggg tggtgataaa gcagacgcgt ggactgctgg gctaaaatac
     4981 gatgctaaca atatttacct agcaaccatg tattcagaaa cgcgtaatat gaccccgttt
     5041 ggcgacagcg attatgcagt tgcgaacaaa acccagaact ttgaagttac tagtaccagt
     5101 ttgatttttg tctgcgtccg gcagtctctt tcctgatgtc taaaggccgt gaccttcacg
     5161 ctgctggtgg tgcagacaac ccggcaggtg ttgatgataa agatctggtt aaatacgccg
     5221 atgttggcgc gacttactat ttcaataaaa acatgtccac ctatgttgac tataaaatca
     5281 acctgttgga tgaagatgac agtttctacg ctgccaacgg catctctacc gatgatattg
     5341 tcgctttagg tctggtttat cagttctaaa tcctcctgcc cgctgttatg gcgggctttt
     5401 tctgcttatt cttcctcctt tgatctaaat taaaaatgtg aactccgtca ttacacaaaa
     5461 agtgtcatct ggcgttacac tttatgcgga tactaaaaca ggaggtttta tgaacagaac
     5521 gattcttgtc cctatcgata tttcagattc agaattaact caacgcgtga ttagccacgt
     5581 tgaggcagag gcaaagattg atgatgcaga ggttcatttc ctgacggtaa taccttcact
     5641 gccctactat gcctctctgg gtttagcgta ttccgcagaa ttaccggcaa tggatgacct
     5701 gaaagcggaa gccaaatcgc aactggaaga gatcattaaa aaatttaaac tgccaaccga
     5761 cagagtgcat gtccatgttg aggaaggctc gcccaaagac cgcattctgg aattggcgaa
     5821 gaagatcccc gctcatatga tcatcattgc ttcccatcga ccggatatca ccacttatct
     5881 gctcggttcc aacgccgcag ctgtagtgcg tcacgcagag tgctccgtgc tggttgtgcg
     5941 ctgacactaa cgcccgcaca ttgctgcggg ctttttgatt catttcgcaa atgtgctgac
     6001 attttcccct ctaatccgta ccatacacgc cacagttttt atatcagatt tcttatgctg
     6061 agcgttccgg catgacgcat actcttctga tgccatataa cgaattgagt cgcttttaaa
     6121 tgtcgcaaaa tcaagaaatt agtaagaaag aacaatacaa cctgaacaaa ttacaaaaac
     6181 gtctgcgtcg taacgtgggc gaagccattg ctgacttcaa tatgattgaa gaaggcgatc
     6241 gcatcatggt ttgcctctcc gggggtaaag acagctatac catgctggag attctgcgca
     6301 atttgcagca aagcgcgcca atcaattttt cgctggtggc tgttaacctc gatcaaaagc
     6361 aaccgggctt cccggaacac gttctgcccg agtatcttga aaagctgggc gttgagtaca
     6421 agattgttga agagaacact tacggtatcg tgaaagagaa gattccagag ggcaaaacca
     6481 cttgctcact gtgttctcgg ctacgtcgcg gtatccttta tcgcaccgca acggaactgg
     6541 ggacgacgaa gatcgcgttg ggtcaccatc gtgacgatat cctgcaaacg ttgttcttaa
     6601 atatgttcta cggcggcaag atgaaaggta tgcctccgaa actgatgagc gatgatggca
     6661 aacatatcgt tattcgtcca ctggcctact gccgcgagaa agatattcag cgatttgccg
     6721 atgcaaaagc gttcccgatt attccgtgca acctgtgcgg ttcacagcct aacctgcaac
     6781 gtcaggtgat tgctgacatg ttgcgtgact gggataaacg ttatccaggg cgtatcgaga
     6841 cgatgttcag cgcgatgcag aatgtggtgc cgtcgcatct gtgcgatacc aacctgttcg
     6901 atttcaaagg cattacccac ggttctgaag tggttaacgg gggtgatctg gcgtttgatc
     6961 gcgaagagat cccactacaa ccggcgggct ggcagccaga agaagatgaa aatcagttgg
     7021 atgagttacg gctgaatgtg gttgaagtga aataaccagg atagcgcccg atgcgcaagc
     7081 gtatcgggct actcttatgg aggccggata agacgcggcc agcgtcgcat ctggcaatcc
     7141 cgaataagat gtttactctt gcacccggca attcaacatt tcattatttt aataaccgca
     7201 cccggcacgt ttttccttta atcttcccgc cctgtaactg tttccatgct ttatgagcaa
     7261 cagcctgacg gaccgcgaca tagacatgcg ccggatgcac ggcgattttg ccaatatctg
     7321 cgccatcaag cccgatatct cctgtcagtg cacctaatac atcacccggg cgcattttgg
     7381 cttttttccc gccatcgata cacaacgttg ccatttctgc ttccagcgtc acaatggaac
     7441 tattagctgg cggcgtttgc cagttaagtt ttatctgcaa catgtcagaa atgatattgg
     7501 cccgctgtgc ttcttccgga gcacagaaac tgatcgccag accgctattt cctgcacgag
     7561 ctgtacgacc gatgcgatgt acatgaactt cagggtccca cgccagctca aagttcacca
     7621 ccagctcaag cgatttaata tccagaccac gcgcagcaac atcagtcgca accagtacac
     7681 gggcgctacc gttagcaaaa cgtaccaggg tctgatcgcg ttgctccaga tcgccgtgta
     7741 acgacaatgc actttgctct acttcattca gcgcgtcgca gacagcctgg caatcttttt
     7801 tggtattgca aaacaccaca caagaggatg gctgatgcaa gcttaataac cgttgcaaca
     7861 gaggaatttt gcctttactg gatgtctcat aaaattgttg ttcaatgggt ggcaaagcat
     7921 ctgttgagtc aatttcaatc gccaaaggat cgcgttgcac tcgtccgctg attgcggcga
     7981 tggcttccgg ccaggttgcc gaaaacagaa gcgtctgtcg agatgcaggc gcaaaacgga
     8041 tgacatcatc aatggcatcg ctaaatccca tatccagcat gcggtcggcc tcatccatca
     8101 ccaaagtatt cagcgcatcc agtgataccg tgcctttttg caagtgatcc agcaaacgac
     8161 ccggcgttgc cacgataata tgcggcgcat gttgcagcga atcacgctgc ataccgaacg
     8221 gttgaccacc gcacaacgtc aaaattttgg tatttggcag aaaacgcgcc agccgacgca
     8281 attcacctgc cacctgatcc gccagttcac gcgtaggaca cagcactaaa gcctgggttt
     8341 gaaatagcga cgcatcaatt tgctgtaaca agccgaggcc aaaagccgcc gttttgccgc
     8401 tgccggtttt cgcctgcacg cgaacatctt ttccggcaag gatcgccgga agcgcggcgg
     8461 cctgcaccgg cgtcatggtt aaataaccca actcattaag gttcgtgagt tgggcgggag
     8521 gcaaaacatt cagggtagaa aaagcggtca caatctattc tcgtggtcat cgacgcaaag
     8581 ttagcaggcg cgtatcctcg cagatctacg ctcacgatgc gacaatttaa tcggttcttc
     8641 atcgggtggt gggtcaggca tgggttgcgg gcgagggatc ggatcgggca ctggaacagg
     8701 atcgccagga atcggttcag ggacaggaat ttgcaaataa ataagtgtcg tcatatttcc
     8761 ctccggtcat tgggtggact cttaaagggt agacgctgat aaataacagg caaaaaaaag
     8821 ccgactcatc aaagtcggcg tcgtacgaat caattgtgct atgcagtaat tcaaaaaagg
     8881 aagtaagaca atatggagcg caacgcccat cgcttgacgt tgcattcacc tgcaagggag
     8941 atattgccct gaatgggtag agagtttatt gacttcgctc aaactttgcg gcgtttttgt
     9001 atacagacag ccggaaaaat tgcttttgtt acaaccattt actacggtgc aaccataaag
     9061 caacaccacc aataagaaca actaacagaa tacaaaaaat tgaaaatccg aattgccacc
     9121 cgccgccagg gatcccacca aggttgacgc caaataaccc ggtcagaaag gtactgggta
     9181 aaaagaccat tgccatcaac gacattgtat aggtacgacg agctaaattt tcctgcatca
     9241 cctgagcgat ttcatccgcc atcacgccag tccgtgctat acaggcgtcg atttcgtcaa
     9301 ggccgcgccc aaggcgatcg gcaatatcct gcatccgacg gcgttggtca tcgctcatcc
     9361 acggcaaacg ttcactggca agacgagcat aaacatcacg ttgcggtgcc atatagcgac
     9421 gcatcacaat taattgtttg cgcagcagag ccaggaatcc acgcggtgga atttgctgat
     9481 caaggagatt atcttcaagg tcgataattt tatcgtgcag ctgctcgata aattcactgg
     9541 aatgatcggt caacgcatcg cacacatcca ccagccatcc cccgcaatcg gtcggacccg
     9601 tgccctcttc cagatcgctc accacatcgt ccagcgccag cactttgcgt tgtcgggtcg
     9661 aaacaattaa ccgcccgtcc atatatacac gcatggcgac cagttgatcg gggcgttcat
     9721 cggtgctgcc gtttatacag cgcaatgtaa tcagcgtgcc ttcaccgaaa cggctgactc
     9781 ggggccgtgt gctctcgccc gccagcgcat cacgtacgtt attgggaagc agcggtgttg
     9841 tcgccagcca ttgggcgcta tcatggtgta cataattaag gtggagccag cagggatgcg
     9901 cttcatcaat cacatctgta ttttccagcg gtttaacgcc gcctctacca tccagcatcc
     9961 aggcaaatac tgcatccggg acattaacgt ccgatccctt aatcgcttcc actgtgcctc
    10021 catcatcaac gcattatttt gtagtctagc cttctggccc tgttacgcaa catctcatca
    10081 ccccattacc ctgaaatgat taataaaatt ctgtctaaat tgaatacaaa aagcaaaatg
    10141 cttttccgta tacaaaccgt gtgaagtgtt aaatagcgtc tatcattatc agaattatct
    10201 gatcatatga cgtggctttt ttgcgatcgg atagcaacaa aaattgataa aaataacggg
    10261 atctcaatga ttacgcacaa cttcaatacc ctggacttac tcaccagtcc tgtctggatt
    10321 gtttcgccct ttgaggaaca gttaatttat gccaatagcg cggcgcgact gttgatgcaa
    10381 gacctcacgt ttagtcagct acgaaccgga ccctattccg tctcctcaca aaaagaactg
    10441 ccgaaatacc tctccgatct gcaaaaccaa cacgatatta tcgaaatcct cactgttcag
    10501 cgtaaagaag aggaaacagc attaagctgt cggcttgttt tgcgagagct gacagaaaca
    10561 gaaccggtga ttattttcga aggtatcgaa gcgccggcaa cgctgggttt aaaagccagt
    10621 cgctcggcaa attatcagcg caaaaaacaa ggtttttatg cgcgcttttt tctgactaac
    10681 tctgcaccaa tgttgttgat tgacccgtca cgagatggac aaatcgtcga tgctaacctc
    10741 gccgcgctca atttctatgg ttataaccat gaaacgatgt gccagaaaca tacctaggaa
    10801 ataaatatgc ttgggcgtcg cgtcatgcct atcatgcatg aaatttcgca tttacccggc
    10861 ggtcataacc ctttgaattt tgttcataaa ctggcggatg gatctactcg tcatgtgcag
    10921 acctatgccg gaccgattga aatttatggc gacaagctca tgttatgtat tgtgcatgat
    10981 attactgagc aaaaacggct ggaggagcag ctggaacatg ctgctcacca tgacgcgatg
    11041 accggattac tgaatcggcg acagttttat cacattacgg aaccaggcca aatgcagcat
    11101 ctcgccatcg ctcaggatta cagcttgttg ctcatcgaca ccgatcgttt taaacacatt
    11161 aacgatctct atgggcattc taaaggtgat gaggtgttat gcgccctcgc ccgccccctc
    11221 gaaagttgcg ctcgcaaagg cgatttggtg tttcgttggg gaggcgaaga gtttgtctta
    11281 ttgctaccaa gaaccccact ggataccgcg ctttcgctgg ctgaaactat ccgcgtaagc
    11341 gtggcaaaag tgagtatttc gggcttacca cgctttaccg tcagcattgg tgtggcgcat
    11401 cacgaaggaa atgaaagcat cgatgaactg tttaaacgcg ttgatgatgc tttgtatcgg
    11461 gcgaaaaatg atggacgcaa ccgcgtgctg gcggcataag ccgcggatgc gtctcgagat
    11521 caacgactgc gcttagcgtg gcgctcccag ttttcttgct tcgcctgcgc cgttttacgt
    11581 agtgcgacgt aacacgcccc gctgccgcca tgatgcggta gcgcggtgca atatgcctga
    11641 acatcatcaa attcggtcag ccagcgcgcc acatagctgc ggacaatatt ggcatgtgat
    11701 ttatcttccc gccctttacc atgaataatc agcacattac gcaaaccatc cgccagggct
    11761 tgttgaatga aactgaacac cattttgcgg cactcttcca ccggctggcg caaaagattc
    11821 aggctcgcct gttgcggata tttaccactg cgcagcttat ccagcacccc atgctacaac
    11881 ccttcctgcc gaaactccag cggctgactt agcgggatga tgtcgagaaa tccggtagtg
    11941 aggaaattat caagctgcag cgtgtcgata cgctgcggcg cacgttggtt acgcgttgga
    12001 tgccagtgga catctgtagc acgtttcagc ggctggacat cttccatggc gtcaagaaac
    12061 agcgatttgt cgtcaaggtt catgtaacct cctatgcacc ttcacacgtc gtgattcaga
    12121 cgggttaatt gttcatactt actgcatcgt tcccttcaca gattgaaaca atactctact
    12181 tcatacctgt gagtatctgc gcaggcgggg cattttatac tagcctgtta gcagtatcga
    12241 acagtttagc ccgagtttaa ttcagcacag gctccgatca ggatgattta ttcatagcag
    12301 gaaccaacaa aaatgcgata aataacagac cactgattag cagaaaggta ccgaaaatgc
    12361 catagtttgc gcctccctca gcatcaattg actcaataat ttggttgaag ccagtatttc
    12421 cgatgtctgg tcacttctac tgcgattaaa aaataaagca cttctggaaa tgaagtcatt
    12481 caccgtatgg ggaacagaaa ccattttttc acccattctc gaaaaatgaa aattagttcg
    12541 attattgaga atagagccac acagggcaat accaatcgcg cccccaagat tacgtgttaa
    12601 attaaataca ccagaggcta acttcagccg ttcttttgga attcccccca gcgttaatgt
    12661 tacgatgggg gccatcgcaa actgctgcga aatccctcta attgcctgtg gaaacagtaa
    12721 ttcctgccat ccccattcgt gggttatagg ggtaaaaaga tacattgaaa acacaaatcc
    12781 gcccagcccc gccatcaaca gccatcgcag atttattttt ttacttaacc agaagtaaaa
    12841 gggaacagaa aataactgga aaatacctgt tgtgcaaacg gcaaaaccta tctcttcggc
    12901 atttaatccg cgaatctgcc ccaaaaacac aggtattaaa tatacagtag agaatatccc
    12961 aacccctcca gaaaaagaaa aaaaacatcc cagagtgaaa tttttatcct taaaagcatg
    13021 aagatccatt atcggattgg atattgtaag tgtacgcgca gcaaataaaa taaaactcac
    13081 caatgccaga actgatgtca gcaaaatagt attatcatcc agccatcccc agcgcgctcc
    13141 ttcttcaagg gtatattcca gacatcccag agtcatggcc aacaggataa tactcggata
    13201 atcagcaact ttcagtaaag agagatcagg tttatcaaaa ttaaccagaa aaggaatact
    13261 caaaactaaa tatatgccag gtaatatatt gatataaaat agccatcgcc agtcgaggtt
    13321 atcagtgagc cagcccccca gagttggccc tagcgtcggt gacagtgagg ccagcgcact
    13381 gactaccgca gcagccaacc ccaactcttt accctggtaa taaataaacg ccatcgtgaa
    13441 aactaaaggg atcatggatg cgccagcagc tccctgtaaa gcacgaaaca aaatcatgat
    13501 ctgaatattc catgcaagtc cacacgcgat actcattaaa gtaaatattc ccgcggatag
    13561 agtaaaaagc caacgagtgg aaaatacccg tgacagccac ccagagagcg gaataacaat
    13621 aatctcagca attaaatagc tggtctgaag ccaggccatt tcgtctttac ctgcggacag
    13681 accaccacct atttcattta gggatgacga tacaatttga atatcaagct gagcaataaa
    13741 taatcccata cacatactgg cgaaagcaat atgtttacgc atagacatca tctctcatca
    13801 acactgacgg tgacggacag tcccgggcga agaagttgct ttaattcctt tgcgtcgtca
    13861 aatgcaatcc gtaccgggac acgctgaaca attttggtga aattgccagt ggcattttct
    13921 atcggaatca agctgaaact ggctcctgtc gctggggaaa ggctttcaat gtgaccgtga
    13981 aacacttccc ctttgagaat atcagcacgg atctcagcct tcattcctgg cttcattccc
    14041 gctatttggt tttctttata atttgcatca acccacaact cactgactgg tactagtgaa
    14101 actaacgatg tgccgccttc cacccaactg cctgtatggg cgctgcggtt tgcgacaatg
    14161 ccatcaatcg gcgca
//
LOCUS       DAJTQQ010000094        13467 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000094 DAJTQQ010000000
VERSION     DAJTQQ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.094"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(220..483)
                     /gene="rpsT"
                     /locus_tag="OO309_003191"
     CDS             complement(220..483)
                     /gene="rpsT"
                     /locus_tag="OO309_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR5848236.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            586..804
                     /locus_tag="OO309_003192"
     CDS             586..804
                     /locus_tag="OO309_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR5848237.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            812..1753
                     /gene="ribF"
                     /locus_tag="OO309_003193"
     CDS             812..1753
                     /gene="ribF"
                     /locus_tag="OO309_003193"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR5848238.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1796..4612
                     /gene="ileS"
                     /locus_tag="OO309_003194"
     CDS             1796..4612
                     /gene="ileS"
                     /locus_tag="OO309_003194"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR5848239.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4612..5106
                     /gene="lspA"
                     /locus_tag="OO309_003195"
     CDS             4612..5106
                     /gene="lspA"
                     /locus_tag="OO309_003195"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR5848240.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5193..5642
                     /gene="fkpB"
                     /locus_tag="OO309_003196"
     CDS             5193..5642
                     /gene="fkpB"
                     /locus_tag="OO309_003196"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5848241.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5644..6594
                     /gene="ispH"
                     /locus_tag="OO309_003197"
                     /gene_synonym="lytB"
     CDS             5644..6594
                     /gene="ispH"
                     /locus_tag="OO309_003197"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR5848242.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6660..7574
                     /gene="rihC"
                     /locus_tag="OO309_003198"
     CDS             6660..7574
                     /gene="rihC"
                     /locus_tag="OO309_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR5848243.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            7741..8562
                     /gene="dapB"
                     /locus_tag="OO309_003199"
     CDS             7741..8562
                     /gene="dapB"
                     /locus_tag="OO309_003199"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR5848244.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            9018..10166
                     /gene="carA"
                     /locus_tag="OO309_003200"
     CDS             9018..10166
                     /gene="carA"
                     /locus_tag="OO309_003200"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR5848245.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            10184..13405
                     /gene="carB"
                     /locus_tag="OO309_003201"
     CDS             10184..13405
                     /gene="carB"
                     /locus_tag="OO309_003201"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR5848246.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
BASE COUNT         3130 a         3394 c         3788 g         3155 t
ORIGIN      
        1 ttgtggggga gtctggagat tgagtagata ttcttgttca gaatgtatca gccgatggtt
       61 ctacgattct taagccacga agagttcaga tagtacaacg gcatgtctct tttgactatc
      121 tggcaaccgg cagtgtgttc tctcacgcat cacaaaagca gcaggcataa aaaaacccgc
      181 ttgcgcgggc tttttcacaa agcttcagca aattggcgat taagccagtt tgttgatctg
      241 tgcagtcagg ttagccttat gacgtgcagc tttgtttttg tggatcagac ctttagcagc
      301 ctgacggtcc acgatcggtt gcatttcgtt aaatgctttc tgtgcagcag ctttgtcgcc
      361 agcttcgata gctgcgtata ctttcttgat gaaagtacgc atcatagagc gacggcttgc
      421 gttgtgctta cgagcctttt cagactgaat ggcgcgcttc ttagctgatt tgatattagc
      481 caaggtccaa ctcccaaatg tgttctatat ggacaattca aaggccgagg aatatgccct
      541 tttagccttc ttttgtcaat ggatttgtgc aaataagcgc cgttaatgtg ccggcactcg
      601 ttacgtagtg atggcgcagg attctaccag cttgcggggt gtgaatacag cttttccgcg
      661 ataaaaattg cagcaggcgg tcagtttctt cccgtgattt gctccatggc aatgaaaagc
      721 cacttctttc tgatttcggt actcaatcgc cggttaacct tgaccgctgt acaaggtata
      781 ctcggacgat tttcactgtt ttgagccaga catgaagctg atacgcggca tacataatct
      841 cagccaggcc ccgcaagaag ggtgtgtgct gactattggt aatttcgacg gcgtgcatcg
      901 cggtcatcgc gcgctgttac agggcttgca ggaagaaggg cgcaagcgca acttaccggt
      961 gatggtgatg ctttttgaac ctcaaccact ggaactgttt gctaccgata aagccccggc
     1021 aagactgacc cggctgcggg aaaaactgcg ttaccttgca gagtgtggcg ttgattacgt
     1081 gctgtgcgtg cgtttcgaca ggcgttttgc ggcgttaacc gcgcaaaatt tcatcagcga
     1141 tcttttggtg aagcatttgc gcgtaaaatt tcttgccgta ggtgatgatt tccgctttgg
     1201 cgctggtcgt gaaggcgatt tcttgttatt acagaaagct ggcatggaat acggcttcga
     1261 tatcaccagt acgcaaactt tttgcgaagg tggtgtgcgt atcagcagca ccgccgtgcg
     1321 tcaggcgctg gctgatgaca atctggctct ggcagaaagt ttactggggc acccgtttgc
     1381 catctccggg cgtgtagtcc acggtgatga attagggcgc actataggtt tcccgacggc
     1441 gaatgtaccg ctacgccgtc aggtttcccc ggtgaaaggg gtttatgcgg tagaagtgct
     1501 gggcctcggt gaaaagccgt tacccggcgt ggcaaacatc ggaacacgcc caacggttgc
     1561 cggtattcgc cagcagctgg aagtgcattt gttagatgtt gcaatggacc tttacggtcg
     1621 ccatatacaa gtagtgctgc gtaaaaaaat acgcaatgag cagcgatttg cgtcgctgga
     1681 cgaactgaaa gcgcagattg cgcgtgatga attaaccgcc cgcgaatttt ttgggctaac
     1741 aaaaccggct taagcctgtt atgtaatcaa accgaaacac ggaaccgaga atctgatgag
     1801 tgactataaa tcaaccctga atttgccgga aacagggttc ccgatgcgtg gcgatctcgc
     1861 caagcgcgaa ccgggaatgc tggcgcgttg gactgatgat gatctgtacg gcatcatccg
     1921 tgcggctaaa aaaggcaaaa aaaccttcat tctgcatgat ggccctcctt atgcgaatgg
     1981 cagcattcat attggtcact cggttaacaa gattctgaaa gacattatcg tgaagtccaa
     2041 agggctttcc ggttatgact cgccgtatgt gcctggctgg gactgccacg gtctgccgat
     2101 cgagctgaaa gtagagcaag aatacggtaa gccgggtgag aaattcaccg ccgccgagtt
     2161 ccgcgccaag tgccgcgaat acgcggcgac ccaggttgac ggtcaacgca aagactttat
     2221 ccgtctgggc gtgctgggcg actggtcgca cccgtatctg actatggact tcaaaactga
     2281 agccaacatc atccgcgcgc tgggcaaaat catcggcaat ggtcacctgc acaaaggcgc
     2341 gaagccggtg cactggtgcg tagactgccg ttctgcactg gcagaagcgg aagttgagta
     2401 ttacgacaaa acttctccgt ccattgacgt cgctttccag gcggtcgatc aggatgcgct
     2461 gaaagcgaaa tttggcgtaa gcaacgttaa cggcccaatc tcgctggtga tctggaccac
     2521 tacgccgtgg actctgcctg cgaaccgcgc aatctctatt gcacctgatt tcgactatgc
     2581 gctggtgcag atcgacggtc aggccgtgat tctggcgaaa gatctggttg aaagcgtaat
     2641 gcagcgtatc ggcgtgaccg attacaccat tctcggcacg gtaaaaggtg cagagcttga
     2701 gctgctgcgc tttacccatc cgtttatggg cttcgacgtt ccggcaatcc tcggcgatca
     2761 cgttaccctg gatgccggta ccggtgccgt tcacaccgcg cctggccacg gcccggacga
     2821 ctatgtgatc ggtcagaaat acggcctgga aaccgctaac ccggttggcc cggacggcac
     2881 ttatctgccg ggcacttatc cgacgctgga tggcgtgaac gtcttcaaag cgaacgacat
     2941 cgtcgttgcg ctgctgcagg aaaaaggcgc gctgctgcac gttgagaaaa tgcagcacag
     3001 ctatccgtgc tgctggcgtc acaaaacgcc gatcatcttc cgcgcgacac cgcagtggtt
     3061 cgtcagcatg gatcagaaag gtctgcgtgc gcagtcactg aaagagatca aaggcgtgca
     3121 gtggctcccg gactggggcc aggcgcgtat cgagtcgatg gttgctaacc gtcctgactg
     3181 gtgtatctcc cgtcagcgca cctggggcgt accgatgtca ctgttcgtgc acaaagacac
     3241 ggaagagctg catccgcgta ccctcgaact gatggaagaa gtggctaaac gcgttgaagt
     3301 tgacggcatc caggcgtggt gggatctcga tgcgaaagag atcctcggcg acgaagctga
     3361 ccagtatgtg aaagtgccgg atactctcga cgtatggttt gactccggat ctacccactc
     3421 ttctgttgtt gacgtgcgtc cggaatttgc cggtcacgca gcggacatgt atctggaagg
     3481 ttctgaccag caccgtggtt ggttcatgtc ttccctaatg atctccaccg cgatgaaagg
     3541 caaagcgccg tatcgtcagg tactgaccca cggctttacc gtggatggtc agggtcgcaa
     3601 gatgtctaaa tccatcggca acaccgtttc gccgcaggat gtgatgaaca aactgggcgc
     3661 ggatattctg cgtctgtggg tggcatcaac cgactacacc ggtgaaatgg ccgtttctga
     3721 cgagatcctg aaacgtgctg ccgatagcta tcgtcgtatc cgtaacaccg cgcgcttcct
     3781 gctggcaaac ctgaatggtt ttgatccagc aaaagatatg gtgaaaccgg aagagatggt
     3841 ggtactggat cgctgggccg taggttgtgc gaaagcggca caggaagaca tcctcaaggc
     3901 gtacgaagca tacgatttcc acgaagtggt acagcgtctg atgcgcttct gctccgttga
     3961 gatgggttcc ttctacctcg acatcatcaa agaccgtcag tacaccgcca aagcggatag
     4021 cgtggcgcgt cgtagctgcc agactgcgct gtatcacatc gctgaagcgc tggtgcgctg
     4081 gatggcacca atcctctcct tcaccgctga tgaagtgtgg ggctatctgc cgggcgaacg
     4141 tgaaaaatac gtcttcaccg gtgagtggta cgaaggcctg tttggtctgg cagacagtga
     4201 agcaatgaac gatgcgttct gggacgagct gttgaaagtg cgtggcgaag tgaacaaagt
     4261 cattgagcaa gcgcgtgccg acaagaaagt gggtggctcg ctggaagcgg cagtaacctt
     4321 gtatgcagaa ccggaactgg cggcgaaact gaccgcgctg ggcgatgaat tacgatttgt
     4381 cctgttgacc tccggcgcta ccgttgcaga ctataacgac gcacctgctg atgctcagca
     4441 gagcgaagta ctcaaagggc tgaaagtcgc gttgagtaaa gccgaaggtg agaagtgccc
     4501 acgctgctgg cactacaccc aggatgtcgg caaggtggcg gaacacgcag aaatctgcgg
     4561 ccgctgtgtc agcaacgtcg ccggtgacgg tgaaaaacgt aagtttgcct gatgagtcaa
     4621 tcgatctgtt caacagggct acgctggctg tggctggtgg tagtcgtgct gattatcgat
     4681 ctgggcagca aatacctgat cctccagaac tttgctctgg gggatacggt cccgctgttc
     4741 ccgtcgctta atctgcatta tgcgcgtaac tatggcgcgg cgtttagttt ccttgccgat
     4801 agcggcggct ggcagcgttg gttctttgcc ggtattgcga ttggtattag cgtgctcctg
     4861 gcagtgatga tgtatcgctc gaaggccacg cagaagctaa acaatatcgc ttacgcgctg
     4921 attattggcg gcgcgctggg caacctgttc gaccgcctgt ggcacggctt cgttgtcgat
     4981 atgatcgact tctacgtcgg cgactggcac ttcgccacct tcaaccttgc cgatactgcc
     5041 atttgtgtcg gtgcggcact gatcgtgctg gaagggtttt taccttccaa agcgaaaaaa
     5101 caataataaa tcctgccgga tgcggcctac agattgctac gaaatcgtag gccgcatccg
     5161 gcaaaaatcc tcaaaaataa gagcaaacct gcatgtctga atctgtacag agcaatagcg
     5221 ccgtcctggt gcacttcacg ctaaaactcg acgatggcac caccgccgag tctactcgca
     5281 acaacggtaa accggcgctg ttccgcctgg gcgatgcttc tctttctgaa gggctggagc
     5341 aacacctgct ggggctgaaa gtgggcgaca aaaccacctt ctcgctggag ccagatgcgg
     5401 cgtttggcgt gccgtcaccg gacctgattc agtacttctc ccgccgtgaa tttatggatg
     5461 caggcgagcc agaaattggc gcaatcatgc tttttaccgc aatggatggc agtgagatgc
     5521 ctggtgtgat ccgcgaaatt aacggcgact ccattaccgt tgatttcaac catccgctgg
     5581 ccgggcagac cgttcatttt gatattgaag tgctggaaat cgatccggca ctggaggcgt
     5641 aacatgcaga tcctgttggc caacccgcgt ggtttttgtg ccggggtaga ccgcgctatc
     5701 agcattgttg aaaacgcgct ggccatttac ggcgcaccga tatatgtccg tcacgaagtg
     5761 gtgcataacc gctacgtggt cgatagcttg cgcgagcgtg gggctatctt tattgagcag
     5821 attagcgaag tgccggacgg cgcgatcctg attttctccg cacacggtgt ttctcaggcg
     5881 gttcgtaacg aagcgaaaag ccgcgatttg acggtgtttg atgccacctg tccgctggtg
     5941 accaaagtgc atatggaagt tgcccgcgcc agtcgccgtg gcgaagaatc tattctcatc
     6001 ggccacgccg ggcacccgga agtggaaggg acgatggggc agtacagcaa ccccgaaggg
     6061 ggaatgtatc tagtcgaatc gccagacgat gtgtggaaac tgacggtcaa aaacgaagag
     6121 aagctctcct ttatgaccca aaccacgctg tcggtggatg acacgtctga tgtgatcgac
     6181 gcgctgcgta aacgcttccc gaaaattgtc ggtccgcgca aagatgacat ctgctacgcc
     6241 acgactaacc gtcaggaagc ggtacgcgcc ctggcagaac aggcggaagt tgtgttggtg
     6301 gtcggttcga aaaactcctc caactccaac cgtctggcgg agctggccca gcgtatgggc
     6361 aaacacgcgt ttttgattga cgatgcgaaa gatatccagg aagagtgggt gaaagaggtt
     6421 aaatgcgtcg gcgtgactgc gggcgcatcg gctccggata ttctggtgca gaatgtggtg
     6481 gcacgtttgc agcagttggg tggtggtgaa gccattccgc tggaaggccg tgaagaaaat
     6541 attgttttcg aagtgccgaa agagctgcgt gtcgatattc gtgaagtcga ttaagtcatt
     6601 agcagcctaa gttatgcgaa aatgccggtc ttgttaccgg cattttttat gaagaaaaca
     6661 tgcgtttacc tatcttcctc gatactgacc ccggcattga cgatgccgtc gccattgccg
     6721 ccgcgatttt tgcccccgaa ctcgacctgc aactgatgac caccgtcgcg ggtaatgtct
     6781 cggttgagaa aactacccgc aatgccctgc aactgctgca tttctggaat gcggagattc
     6841 cgctcgccca gggagccgct gtgccgctgg tacgcgcacc gcgtgatgca gcatctgtgc
     6901 acggcgaatc gggaatggct ggctacgact ttgttgagca caaccgaaag ccactcggga
     6961 taccggcgtt tctggcgatt cgggatgccc tgatgcgtgc accagagccc gttaccctgg
     7021 tggccatcgg cccgttaacc aatattgcgc tgttactttt acaatgcccg gaatgtaaac
     7081 cgtatattcg tcgtctggtg atcatgggcg gttctgctgg tcgcggcaac tgtacaccaa
     7141 atgccgagtt taatatcgct gccgatccag aagcggctgc ctgtgtcttc cgtagcggca
     7201 tcgaaattgt catgtgcggt ctggatgtca ccaatcaggc aatattaacc cctgactatc
     7261 tcgctacttt accggagttg aatcgcaccg gaaaaatgct ccacgccctg tttagccact
     7321 accgtagcgg cagtatgcaa agcggtctgc gaatgcacga tctctgcgcc atcgcctggc
     7381 tggtgcgccc ggacttattc accctcaagc cgtgttttgt cgcggtagaa actcaaggcg
     7441 aatttacctc aggcacgacg gtggttgata tcgacggttg cctgggcaaa ccagccaatg
     7501 tacaggtggc attggatctg aatgtaaaag gcttccaaca gtgggtggct gaggtgctgg
     7561 ctctggtgcc gtaacctgtc acatgttatc ggcatgcagt cattcatcga ctcatgcctt
     7621 tcactgatat ccctccctgt ttatcattaa tttctaatta tcagcgtttt tggctggcgg
     7681 cgtagcgatg cgctggttac tctgaaaacg gtctatgcaa attaacaaaa gagaatagct
     7741 atgcatgatg caaacatccg cgttgccatc gcgggagccg gggggcgtat gggccgccag
     7801 ttgattcagg cggcactggc attagaaggc gtgcagctgg gcgcggcgct ggagcgcgaa
     7861 ggatcttctt tactgggcag cgacgccggt gagctggctg gtgcagggaa aacaggcgtt
     7921 accgtgcaaa gcagtcttga tgcgataaaa gatgattttg atgtgtttat cgattttacc
     7981 cgtccggaag gtacgctgaa ccatctcgct ttttgtcggc agcatggcaa agggatggtg
     8041 atcggcacta cggggtttga cgaagccggt aaacaggcaa ttcgtgacgc cgccgccgat
     8101 attgcgattg tctttgccgc caattttagc gttggcgtta acgtcatgct taagctgctg
     8161 gagaaagcag ccaaagtgat gggtgactac accgatatcg aaattattga agcacatcat
     8221 agacataaag ttgatgcgcc gtcaggcacc gcactggcaa tgggagaggc gatcgcccac
     8281 gcccttgata aagatctgaa agattgcgcg gtctacagtc gtgaaggcca taccggtgaa
     8341 cgtgtgccag gtaccattgg ttttgctacc gtgcgtgcag gtgacatcgt tggcgaacat
     8401 accgcgatgt ttgccgatat tggcgagcgt ctggagatca cccataaggc gtccagccgc
     8461 atgacatttg ctaacggtgc ggtaagatcg gctttgtggt tgagtggtaa ggaaagtggt
     8521 ctttttgata tgcgagatgt gcttgatctc aataatttgt aaccacaaaa tgtttattat
     8581 gatgcaaaag taacacattt aatttattaa ttataaaggg ctttaatttt tagccctttt
     8641 atttttggtg ttatgctttt aaattttcta taactgccaa aaattacatg tttcgtcttc
     8701 tatttttgtt gttttaatgt aaattttgac catttggtcc actttttcct gctcgttttt
     8761 atttcatgca atcttcttgc tgcgcaagcg ttttccagaa caggttagat gatgtttttg
     8821 tcgcttaatg cctgtaaaac atgcatgagc cacaaaataa tataaaaaat cctgccatta
     8881 agttgacttt tagcgcccat atctccagaa tgccgccgtt tgccagaaat tcgtcggtaa
     8941 gcagatttgc attgatttac gtcatcactg tgaattaata tgcaaataaa gtgagtgagt
     9001 attctctgga gggtgttttg attaagtcag cgctattggt tctggaagac ggaacccagt
     9061 ttcacggtcg ggccataggg gcaacaggtt cggcggttgg ggaagtcgtt ttcaatactt
     9121 caatgaccgg ttatcaagaa atcctcactg atccttccta ttctcgtcaa atcgttactc
     9181 ttacttatcc ccatattggc aatgtcggca ctaatgacgc cgatgaagaa tcttctcagg
     9241 tacatgcaca aggtctggtg attcgcgacc tgccgctgat tgccagcaac ttccgtaata
     9301 ccgaagacct ctcttcttac ctgaagcgcc ataacatcgt ggcgattgcc gatatcgata
     9361 cccgtaagct gacgcgttta ctgcgcgaga aaggcgcaca gaatggctgc attatcgcag
     9421 gcgataaccc ggatgcggcg ctggcgttag aaaaagcccg cgcgttccca ggtctgaacg
     9481 gcatggatct ggcaaaagaa gtgaccaccg cagaagccta tagctggaca caagggagct
     9541 ggacgttgac cggcggcctg ccagaagcga aaaaagaaga cgagctgccg ttccacgtcg
     9601 tggcttatga ttttggtgcc aagcgcaaca tcctgcggat gctggtggat agaggctgtc
     9661 gcctgaccat cgttccggcg caaacttctg cggaagatgt gctgaaaatg aatccagacg
     9721 gcatcttcct ctccaacggt cctggcgacc cggccccgtg cgattacgcc attaccgcca
     9781 tccagaagtt cctcgaaacc gatattccgg tattcggcat ctgtctcggt catcagctgc
     9841 tggcgctggc gagcggtgcg aagactgtca aaatgaaatt tggtcaccac ggcggcaacc
     9901 atccggttaa agatgtggag aaaaacgtgg tgatgatcac cgcccagaac cacggttttg
     9961 cggtggatga agcaacatta cctgcaaacc tgcgtgtcac gcataaatcc ctgttcgacg
    10021 gtacgttaca gggcattcat cgcatcgata agccggcgtt cagcttccag ggtcaccctg
    10081 aagccagccc tggtccacac gacgccgcgc cgttgttcga ccactttatc aagttaattg
    10141 agcagtaccg taaaaccgct aagtaatcag gagtaaaaga gccatgccaa aacgtacaga
    10201 tataaaaagt atcctgattc tgggtgcggg cccgattgtt atcggtcagg cgtgtgagtt
    10261 tgactactct ggcgcgcaag catgtaaagc cctgcgcgaa gagggttacc gcgtcattct
    10321 ggtgaactcc aacccggcga ccatcatgac cgacccggaa atggccgatg cgacctacat
    10381 cgagccgatt cactgggaag tggtacgcaa gattattgaa aaagagcgcc cggacgcggt
    10441 gctgccaacc atgggcggtc agacggcgct gaactgcgcg ctggagctgg aacgtcaggg
    10501 cgtgttggaa gagttcggtg tcaccatgat tggtgccact gccgatgcga ttgataaagc
    10561 agaagaccgc cgtcgtttcg acgtagcgat gaagaaaatt ggtctggaaa ccgcgcgttc
    10621 cggtatcgca cacacgatgg aagaagcgct ggctgttgcc gctgacgtgg gcttcccgtg
    10681 cattattcgc ccatccttta ccatgggcgg tagcggcggc ggtatcgctt ataaccgtga
    10741 agagtttgaa gaaatttgcg cccgcggtct ggatctctct ccgaccaaag agttgctgat
    10801 tgatgagtcg ctgatcggct ggaaagagta cgagatggaa gtggtgcgtg ataaaaacga
    10861 caactgcatc atcgtctgct ctatcgaaaa ctttgatgcg atgggcatcc acaccggtga
    10921 ctccatcact gtcgcgccag cccaaacgct gaccgacaaa gaatatcaaa tcatgcgtaa
    10981 cgcctcgatg gcggtgctgc gtgaaatcgg cgttgaaacc ggtggttcca atgtccagtt
    11041 tgcggtgaac ccgaaaaacg gtcgcctgat tgttatcgaa atgaacccac gcgtgtcccg
    11101 ttcttcggcg ctggcgtcga aagcgaccgg tttcccgatt gctaaagtgg cggcgaaact
    11161 ggcggtgggt tacaccctag acgaactgat gaacgacatc actggcggac gtactccggc
    11221 ctccttcgag ccgtccatcg actatgtggt tactaaaatt cctcgcttca acttcgaaaa
    11281 attcgccggt gctaacgacc gtctgaccac tcagatgaaa tcggttggcg aagtgatggc
    11341 gattggtcgc acgcagcagg aatccctgca aaaagcgctg cgcggcctgg aagtgggcgc
    11401 aactggcttc gacccgaaag tgagcctgga tgacccggaa gcgttaacca aaatccgtcg
    11461 cgaactgaaa gacgcaggcg cagagcgtat ctggtacatc gccgatgctt tccgcgcggg
    11521 cctgtctgtg gacggcgtct tcaacctgac taacattgac cgctggttcc tggtacagat
    11581 tgaagaactg gtgcgtctgg aagagaaagt ggcggaagtg ggcatcactg gcctgaacgc
    11641 tgaattcctg cgccagctga aacgcaaagg ctttgccgat gcgcgcttgg caaaactggc
    11701 gggcgtacgc gaagcggaaa tccgtaagct gcgtgaccaa tatgacctgc acccggtcta
    11761 caagcgcgtg gatacctgtg cggcagagtt tgccaccgac accgcttaca tgtactccac
    11821 ttatgaagaa gagtgcgaag cgaatccgtc taccgaccgt gaaaaaatca tggtgcttgg
    11881 cggcggtcca aaccgtatcg gtcagggtat cgaattcgac tactgctgcg tacacgcctc
    11941 gctggcgctg cgtgaagacg gttacgaaac cattatggtt aactgtaacc cggaaaccgt
    12001 ctctaccgac tacgacactt ccgatcgcct ctacttcgag ccggtaactc tggaagatgt
    12061 gctggaaatc gtgcgtattg agaagccgaa aggcgttatc gttcagtacg gcggtcagac
    12121 cccgctgaaa ctggcgcgtg cactggaagc cgctggcgta ccggttatcg gcaccagccc
    12181 ggatgctatc gaccgtgcgg aagaccgtga acgcttccag catgcggttg accgtctgaa
    12241 actgaaacaa ccggcgaacg ccaccgttac tgctattgaa atggcagttg agaaggcaaa
    12301 agagattggc tacccgctgg tggtgcgtcc gtcttacgtt ctcggcggtc gggcgatgga
    12361 aatcgtctat gacgaagctg acctgcgtcg ctacttccag acggcggtta gcgtgtctaa
    12421 cgatgcgcca gtgttgctgg atcatttcct tgatgacgca gtagaagttg acgtggatgc
    12481 catctgcgac ggcgaaatgg tgctgattgg cggcatcatg gagcacatcg agcaggcggg
    12541 tgtgcactcc ggtgactccg catgttcgct gccagcgtac accttaagtc aggaaattca
    12601 ggatgtgatg cgccagcagg tgcagaaact ggccttcgaa ttgcaggtgc gcggtctgat
    12661 gaacgtgcag tttgcggtga aaaacaacga agtttacctg attgaagtaa acccgcgtgc
    12721 ggcgcgtact gttccgttcg tctccaaagc caccggcgta ccgctggcaa aagtggcggc
    12781 gcgtgtgatg gctggcaaat cgctggctga gcagggcgta accaaagaag ttattccgcc
    12841 gtactactcg gtgaaagaag tggtgctgcc gttcaataaa ttcccaggcg ttgacccgct
    12901 gttagggcca gaaatgcgct ctaccgggga agtcatgggc gtgggccgca ccttcgctga
    12961 agcgttcgcc aaagcgcagc tgggcagcaa ctccaccatg aagaaacacg gtcgtgcgct
    13021 gctttccgtg cgcgaaggcg ataaagaacg cgtggtggac ctggcggcaa aactgctgaa
    13081 acagggcttc gagctggatg cgacccacgg cacggcgatt gtgctgggcg aagcgggtat
    13141 caatccgcgt ctggtaaaca aggtgcatga aggccgtccg cacattcagg accgtatcaa
    13201 gaatggcgaa tatacctaca tcatcaacac cacctcaggc cgtcgtgcga ttgaagactc
    13261 ccgcgtgatc cgtcgcagtg cgctgcaata taaagtgcat tatgacacca ccctgaacgg
    13321 tggtttcgct accgcgatgg cgctgaatgc cgatgcgact gaaaaagtaa tttcggtgca
    13381 ggaaatgcac gcacagatca aataatagcg tgtcgtggcg gatatttttc atccgctaat
    13441 ttgatcgaat aactaatacg gttctct
//
LOCUS       DAJTQQ010000095        13367 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000095 DAJTQQ010000000
VERSION     DAJTQQ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13367
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.095"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            1..2523
                     /locus_tag="OO309_003202"
                     /pseudo
     CDS             1..2523
                     /locus_tag="OO309_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            2533..6179
                     /locus_tag="OO309_003203"
                     /pseudo
     CDS             2533..6179
                     /locus_tag="OO309_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6240..6647
                     /locus_tag="OO309_003204"
     CDS             6240..6647
                     /locus_tag="OO309_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848247.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7189..8277
                     /locus_tag="OO309_003205"
     CDS             7189..8277
                     /locus_tag="OO309_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR5848248.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8288..10567
                     /locus_tag="OO309_003206"
     CDS             8288..10567
                     /locus_tag="OO309_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848249.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            10560..11696
                     /locus_tag="OO309_003207"
     CDS             10560..11696
                     /locus_tag="OO309_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR5848250.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            11693..>13367
                     /locus_tag="OO309_003208"
     CDS             11693..>13367
                     /locus_tag="OO309_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848251.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTR"
BASE COUNT         3301 a         3525 c         3439 g         3102 t
ORIGIN      
        1 gtcacgatcg aatgacgaaa gcctgcgctg cgttcccaca cgcagcactg gcagcactgg
       61 cggaacttct agtgcaaaaa gaagagaata gttggcgcat tatgctaatg acgatgttta
      121 tctcgcaacc agcactggca gaacaggtca ttccctggct ttcgacaccc gcagttgcag
      181 tgctgaaatc atgccagcac caactgacac agccctcaaa ccatgccagc gccgatctac
      241 tgccagccat agtagtctcc ccaccatgga tttcgaaaaa gaaaaaatcg ccgattccgg
      301 tgctggattt agcgccatta ggcatcgagc caatctgtta tctgacagaa gaaatcagta
      361 atcaactttt ggcgaaatat atctggtatt caaaacacat cacggttagc catgaagaaa
      421 gtactaccaa cctgttggca aggatgggtt ttcaacgacg gatcgctggt acatatatta
      481 aagctcccga agcggtagtt gaggcatggc taaatgaaga ttattcaacc ttactaagtg
      541 aatttaaggt gtttcattca cctaccgggc attattggca gttggggatt ttgacaacat
      601 tgccgctgga ggctggagaa agcagtaaaa gcatggaatg cccttactct atctccacat
      661 accgataccg aatacgccat gttacatttt ggactcaaag gtttacctgg tttagttaac
      721 tcaattacac gctatccaca ggaagccttg cccatcacga attacttcgc agcgagtgag
      781 ctggctcctg ccgtcgcccg tgccttcaac aaactcaaaa cgctacgcga aaacgcccgc
      841 acctggctgt tgaaataccc ggaacatgcc cttaccggcc tgctgcctgc ggcgctcggc
      901 aaagccggtg aagcacagga taacgcccgc gctgccttgc gtatgcttac cgaaaacggt
      961 catcagccat tactgcaaga aatcgcccgg cgttataacc agtcggaagt aacagacgcg
     1021 gtgaacgctc tgcttgcgct cgatccctta gataatcacc cgacaaaaat ccccactctt
     1081 ccgacctttt atcagccatc gatctggacg cgcccggtat taaaagcaaa cgcccaatca
     1141 ctgccagata acgccctcct ccacctcggc gaaatgctcc gcttccctca ggaagaggct
     1201 ctgtatccgg gattattgca ggtgaaagac gcctgtaccg ctgactctct ggctgaattt
     1261 gcctgggatc tgtttaccgc ctggcagacc gctggcgcgc cgtcgaaaga gagttgggcg
     1321 ttcactgcgt taggcgttct cggtaacgat gacaccgccc gcaaactgac gccattaata
     1381 cgcgcctggc ctggtgaatc ccagcataaa cgcgccaccg ttgggttgga tattctcgcc
     1441 gctatcggta gtgatatcgc cctgatgcag cttaacggca ttgctcagaa actgaaattc
     1501 aaagcattac aggagcgggc aaaagaaaag attgccgaca ttgccgagag ccgcgaactc
     1561 acggtagcgg agcttgaaga tcggttagca ccggatctcg gtctggatga taacggttcg
     1621 ttgctgctgg attttggccc acgccagttc accgtcagct ttgatgaaac cttaaaaccg
     1681 tttgtgcgtg atgtttccgg cagccgcctg aaagacctgc caaaaccaaa caaaagcgat
     1741 tatgagtcac aggcgaacga tgctgttaac cgctacaaat tgctgaaaaa agatgcgcgt
     1801 accgtcgcgg cccagcaggt agcaaggctt gaatccgcca tgtgcctgcg tcgccgctgg
     1861 tcgccggaaa acttccaact cttcctggtt gtgcatccgc tggttcgcca cttaacccgc
     1921 cgtctgattt ggggcgttta tagcgccgac aaccagctac tggcctgttt ccgggtagcg
     1981 gaagataaca gctacagcac cgctgacgat gatcttttca ccctgccgga aggcgatatc
     2041 tctatcggca ttcctcacgt tctggaaata tcaccgacgg atgctgtcgc ctttggtcag
     2101 ctttttgccg actacgaact gctaccaccg ttccgtcagc ttgaccgtaa cagttacgcc
     2161 ctgacagaag ccgagcgcaa cgccagcgaa ctgacccgct gggcaggcag aaaatgcccg
     2221 agcggccggg tcatggggct ggcaaataaa ggctggataa agggcgaacc acaggatgga
     2281 ggctggatga tcaaacctct cggtcgctgg tcgttaatca tggaaatcga cgaaggtttt
     2341 gcggtgggta tatcaccagc cgaacttagc gctgagcagc tcttaagcaa gctgtggcta
     2401 tgggaaggca aagcggaaag ctatggctgg gggagtaatt caacacagga agcgcagttc
     2461 tccgtactcg atgccatcac cgccagcgag ctaattaacg atattgaagc cctgtttgaa
     2521 taaggaaaga gcatggacaa ggaattaccg tggctggcgg ataacgccca actggaactg
     2581 aaatataaaa aaggcaaaac accactcagt catcgcaatt ggccgggcga accagtgcct
     2641 gttatcactg aaagcatcat tcagacactg ggtgatgaat tgctacaaaa agctgagaag
     2701 aaaaaaaaca ttgtctggcg ttatgaaaat ttttcaccgg agtggcagtc cgccattacg
     2761 caggccatca acttgatcgg cgaacacaaa ccctcagtcc cggcccgcac aatggcggca
     2821 ctagccttta tcgcgcaaaa tgacaaccaa cagttgctcg acgaaatcgt ccaacaagag
     2881 gggctggaat atgcgactga ggtggtgatt gcacgccagt ttattgcacg gtgttatgag
     2941 agtgatcctc tggtagtcac attgcagtat cagaacgagg actatggcta tggttatcgc
     3001 tcagaaacct ataacgaatt cgatctccga ctgcgtaaac acctctctct ggcagaggaa
     3061 agctgctggc agcgttgcgc cgacaaactc attgccgcac tgccaggaat aaccaaagtt
     3121 cgccgccctt ttattgcgct gatcctcccc gaaaaaccag aaatcgccaa tgagttggta
     3181 agccttgaat gcccgcggac tcattttcat tctaaggagt ggttaaaagt tgttgctact
     3241 gaccccaaag cggtgagaaa actcgaacgc tactggagcc aggatatatt tagcgatcga
     3301 gaagccagct acatgtcgca tgaaaaccac ttcggctacg cggcctgcgc tgcccttttg
     3361 cgcgaacaag gactggcagt cgttccgcgc ctcgcgatat atgcccataa agaagattgc
     3421 ggcagtctgc tggtacaaat taaccatccg caagtcatcc gcaccttgct gttagttgct
     3481 gataaaaaca aacccagcct gcaacgtgtg gctaaataca gtaaaaactt cccccatgcg
     3541 acactcgccg cactggcaga actgctggcg ttaaaagaac caccagcccg cccaggttat
     3601 ccaatcatcg aagacaaaaa gctgcctgca cagcaaaaag ctcgagatga atactggcgt
     3661 acgctgttac aaacgctgat ggcatcgcag ccacaactgg cagcagaagt gatgccgtgg
     3721 ttaagtactc aagcccgggc agtgctgaat agttatttat cggcaccgcc caaaccagtt
     3781 attgatagca ccgataacag ccaaatgcct gaaatcctcg tttcaccacc atggcgtggg
     3841 agaaagaaaa agacttccat acgtcacttt tcgctgcctg atcttacgct tgcaccgcaa
     3901 acgtacaggt cgccgaataa acactggacg ccggaacagc aaactgcaat tgaccatttt
     3961 agcgccatgt cttttgacga acgtcttgct acgagaggag cggatatctt cttgcgggaa
     4021 ttaggttttg acgttcatag cctggaattt aataagcgtc ttcttaatgg acagttggat
     4081 gcctttgatg ctcagcaact gcaagaatac acatcaatag gactattgcg tacttatcac
     4141 gcgcatcagg ccaaatctaa acagtaccgg gaagacgcca ccaatgcatt actggcacaa
     4201 gatagcattg cgttgatggg agggtggcga acttttcagc acggatttta ctatggtaat
     4261 gaacagcgtt gggggatttg gaatctgtac cttatcgcgc agatgccgcg cgaaatggcg
     4321 gtatcgtgct ggcaacaaat tgtcgaggct gattttcatt acaccggggt ggaatatttg
     4381 ttaagtgtgc tgggtaccga tgctttgcct ggattaaaca ccgcctttgc ccgacatcca
     4441 aaagagatat ttccgctgct gattcatttc ggcgcaacag aactggccct gcccattgcc
     4501 cgcgtctggc acctttttgc gggccagcgt aatctggctc gccagtggat tttacaatgg
     4561 ccggaacata cggctactgc acttatccct cttgtcttca ccaaatctag cgataaaagc
     4621 gaagctgcat tacttgccct gcgtttactt tacgaacatg ggcatggcga atggctacaa
     4681 actgtagcca atcgttggca gcgtacagat ctatggcctg ccctggaaca attacttaaa
     4741 cagagtccaa tagagattta cccggcacgc attccaaaag cccctgattt ctggcatccg
     4801 caaatgtggt ccaggccgcg ccttatcact aataatcaac ctgttaccga tgacgctctg
     4861 gaaattatcg gcgaaatgct gcgctttacc caggggggac gtttttatag cgggctggaa
     4921 caactgaaaa cgttctgcca gccacaaacg ctggcagctt ttgcctggga tctcttcact
     4981 gcgtggcaac aagctggtgc ccccgcaaaa gacaactggg catttctggc gttaagtctc
     5041 tttggtgacg aaagcacggc acgggatcta acgacacata tcctcgcctg gccacaagaa
     5101 ggcaaatctg cccgtgcggt cagtggcatg aacatcctta ccctgatgaa taatgatatg
     5161 gcgctgatgc agctgcatca tatatcgcaa cgggcgaaat cccgcccctt acgtgataac
     5221 gcggcggaat ttcttcaggt agtcgcagaa aatcgcgggc taagccagga agagttagcg
     5281 gacagattag tcccaacact gggccttaat gatccgcagg cgttgagttt tgattttggt
     5341 ccccggcagt ttaccgttcg cttcgatgaa aacctcaacc cggttatctt tgatcagcaa
     5401 aacgttcgcc agaaaagtgt tccccggttg cgcgccgatg acgatcaact gaaagcgccc
     5461 gaggcactgg cccgactaaa agggttaaaa aaagatgcta ctcaggtgag caaaaacctg
     5521 ctcccgcgtc ttgaagctgc cctacgtacc acccgacgct ggtcgctggc agattttcat
     5581 tctctgtttg ttaatcatcc ctttacccgt ctggttaccc agcgattaat atggggggtt
     5641 atccggcaaa tgaaccgcgt cgtttactca acgcctttcg tgtggccgca gagggggagt
     5701 tctgcaatgc gcaagatgag ccaattgacc tgcctgcgga cgctctgatt ggcattgccc
     5761 acccgttaga aatgacagca gaaatgcgca gtgaatttgc acagcttttt gccgattacg
     5821 aaattatgcc gccttttcgc cagttgtcgc gccgcacggt gctgctcaca cctgacgagt
     5881 caaccagtaa cagcctgact cgctgggaag gtaaatccgc taccgttggg caacttatgg
     5941 gaatgcgata caaaggctgg gagtcaggtt atgaggacgc atttgtctat gacctgggtg
     6001 agtaccggct ggtccttaag ttttcacccg gttttaacca ctacaatgtt gatagcaaag
     6061 cgctaatgag cttccgttct cttcgagtgt accgtgacaa taaatccgtc acttttgccg
     6121 aacttgatgt gtttgatttg agtgaggcgt taagcgcacc tgacgtcatt ttccattaaa
     6181 acaaagcggg cagtgatgcg actgcccgtt atctacacga cttaccagcg gggaaagcga
     6241 tgatcgtgca aaaagagctg gttgctattt acgattatga ggtccctgta cctgaagatc
     6301 cgttttcctt cagacttgag atccataaat gctctgaatt atttacaggt tccgtctacc
     6361 gactggaacg gttccggcta cgtccaacat ttcatcaacg tgatcgagag gatgttgacc
     6421 cgctaataaa tgatgcgttg atttatataa gagatgagtt tattgatgag caaaaattac
     6481 gaggcgaatc acctgaacct gtaatagtaa tttttaattg taaactacag aatatattca
     6541 accaacaaat agaattatat actctagata attcaagtcg cagcacatgc aacttgaagt
     6601 atgacgagta tagctttatt aaagggggga acggtattcc cccttagatg cgtttcaatt
     6661 tgcccgttac ttcaacaaat caatcaaccg taacagagcc actaatagct ccagtacagc
     6721 aatgagcacg gttaactttt gtgtcagcgt gtccatccgc tctccttatt aaggagcaca
     6781 acaccagcac cttaccctta cactgcctgt cgatactggt ttacgtttgt tgagtcgcca
     6841 tgttgcactc cggatcagag cactacctgc ggcaccaccc gtccggtagc caggacttga
     6901 aataggttcg atccggcgca acttcccaac agtcaggatt ataaatctca ttatcttaat
     6961 tgcaacactg tccagaggtg gttataattc gttcgttgga acacgatgtt gcacaggcag
     7021 tggtgttagg cctgaaaata gtaaataaaa gaacctgcct caccagcagt tttttttatt
     7081 tactgtgatc tgctttccag atatttttcg ctcaaacaac taatgcgcca aacatttatt
     7141 gcgcgtaaaa tatcgtttat ttcattaata catttcaggg atgaatatat gtcaccgcag
     7201 aataatcatc tgcagcgtcc gcctgctgct gtgttatacg ccgatgaact ggcaaaatta
     7261 aaacaaaatg ataacgcacc ttgcccgccc ggttggcagt taagtttgcc tgcggcccgt
     7321 gcttttatcc ttggcgatag cgcgcaaaat attagccgta aagtggtgat tagtccctcc
     7381 gctgtcgaac gtatgttagt cactcttgcc accggacgcg gtttgatgtt ggtgggggaa
     7441 cctggcaccg caaaatctct cctgtctgaa ttactggcaa cctctattag cggcgatgcc
     7501 gggttaacca tccagggcgg cgcatctact accgaagatc aaatcaagta cggctggaac
     7561 tacgccctgc ttatcaatca tggtccatca acggaagcgc tggtccctgc cccgctttac
     7621 cagggaatgc gcgacggcaa aatcgtccgt tttgaagaga taacgcgtac accgctggaa
     7681 gtgcaagact gtctgttagg tatgctttct gacagggtga tgacggtgcc ggaactcact
     7741 ggtgaagcca gtcagctcta tgcgcgagaa ggctttaata ttattgccac tgccaatacc
     7801 cgcgatcgcg gcgtcaatga aatgagtgct gcgctaaagc gtcgcttcga ttttgaaact
     7861 gtctttccga ttatggattt cgcccaggag ctggagcttg tcgccagtgc ttcggcgcgt
     7921 ttgctggccc atagcggtat tccgcataaa gtgcctgacg cggtactgga gttactggtc
     7981 cgcaccttcc gcgatctgcg tgccaacggc gaaaagaaaa cgtcaatgga tacactaaca
     8041 gcgattatgt ccaccgctga agccgtgaac gtggcccacg ccgtaggcgt ccgcgcctgg
     8101 tttttagcga atcgcgcagg tgagccagcc gacctggtgg actgcattgc cggaaccatc
     8161 gtcaaagata acgaagaaga tcgcgctcgt ctgcgccgtt acttcgaaca gcgtgtcgcc
     8221 acacataaag aagctcactg gcaggcttat tatcaagccc gccaccgcct gccgtgagga
     8281 aagatgcatg agcgagccgt taattgtcgg catccggcat catagtccgg cctgcgcccg
     8341 gctggtgaaa tcgttaatcg aaagccagcg gccacgatac gtgttgattg aaggccctgc
     8401 tgattttaat gaccgggtag acgaactgtt tttagcccac cagcttccgg tagctattta
     8461 cagttattgc cagtatcagg acggtgcagc ccccgggcgt ggtgcctgga cgccatttgc
     8521 tgaattttcg ccggagtggc aggcgctaca agccgatcgt cgtattcagg cacaaactta
     8581 cttcatcgat ttgccttgct gggcgcagag tgaagaagag gacgattcgc ctgatacgca
     8641 agaggaaagc cagaccttgc tgctgcgtgc cacccgcatg gataacagcg acaccctgtg
     8701 ggatcacttg ttcgaagatg aaagccagca aactgcatta ccctctgcac tggcgcacta
     8761 ttttgcccaa ctgcggggcg actcccccgg cgatgctcta aatcgtcagc gcgaagcctt
     8821 tatggcccgc tggattggat gggcgatgca gcaaaataat ggcgacgtgt tagttgtctg
     8881 cggtggctgg cacgctccgg cactggcaaa aatgtggcgc gaatgtccgc aggtaattaa
     8941 cacgccagaa ttgccgtcgc tggcagatgc cgttacaggt tgttatctca ccccctacag
     9001 tgaaaagcgc cttgatgtgc tggcaggata cctttcagga atgcctgccc cggtctggca
     9061 aaactggtgc tggcagtggg gcttacagca ggcgggagaa caactgctaa aaacggttct
     9121 cacccgtttg cgccagcaca agttgcctgc ttcgaccgcg gatatggctg ccgctcatct
     9181 gcatgcgatg gcactggcac agttgcgcgg tcatacacta ccgttacgca ctgactggct
     9241 ggatgccata gcaggatcgc tgattaaaga agccctgaat gcgccgttgc cgtggagcta
     9301 tcgcggcgtt attcatcccg ataccgatcc gattctgcta acgttgatag acacattagc
     9361 gggtgacgga ttcggtaaac ttgccccttc tacgccacaa ccgcctctgc caaaagatgt
     9421 cacttgcgaa ctggaacgta ccgcaatctc tcttccggcg gagcttacct taaatcgctt
     9481 taaccccaat ggactagcgc aaagtcaggt gttacatcgg ctggcaatac tggagatccc
     9541 agggattgta cgccagcagg gaagcacact gacacttgca ggcaacggtg aagaacgctg
     9601 gaaattaacc cgcccgctta gccagcatgc ggcattgatt gaggccgcct gctttggtgc
     9661 cacactcctg gaagccgcac gccataaatt agaagccgat atgctggacg cgggtggaat
     9721 cggcagcatt accacatgtc ttagccaagc ggcgtcagcg ggtctggcgt ccttcagtca
     9781 acaattactg gagcaactca cattattaat cgcccaggaa aatcaatttg ccgaaatggg
     9841 ccaggcgctg gaagtgctat atgccttatg gcggctggat gaaattagcg gtatgcaagg
     9901 cgcgcagata ttacaaacga cgttatgcgc ggctatcgat cgcacgctgt ggctgtgtga
     9961 atccaacggc agaccggatg aaaaggagtt tcacgctcac ttgcatagct ggcaagcgtt
    10021 gtgtcatatc ctgcgcgatc tacatagcgg tgtgcaactc cccggcgttt cgctttctgc
    10081 ggcggtagcc ttgctggagc gacacagtca ggcgattcat gtcctggcac tggatcgcgg
    10141 cgcgactctt ggtgcgctga tgcgtctgga acatcccaac gctagtgccg aagcggcgct
    10201 gacgatgctg gcgcagttat ccccggcaca atccggcgag gcgctgcacg gtttgctggc
    10261 attagcccgt catcaactgg cctgtcagcc ggcatttatc gccggtttca gcagtcattt
    10321 aaatcaacta agtgatgccg attttaccaa tgccctgccc gatttacgcg cggcaatggc
    10381 ctggctaccg ccacgagaac gcgggacgct ggcgcatcag gtgcttgagc attatcaact
    10441 ggcgcaactt cccgtttcgg cactgcaaat gccgttgcat tgtccaccgc aagccattgc
    10501 acatcatcaa caactcgaac agcaggcact ggcatcgctg caacactggg gagttttcca
    10561 tgtctgaact gaacgatctt ctgaccaccc gtgagctaca acgctggcga ttaattcttg
    10621 gcgaagcggc agaaacgacg ctttgtgggc tggatgacaa cgcccggcag atagatcacg
    10681 cgctggagtg gctgtatggg cgcgatcctg aacggctcca gcgtggtgaa cgctctagtg
    10741 gattaggtgg ctcaaatctc accactcctg agtggatcaa cagtattcac acgctgtttc
    10801 cgcaacaggt gattgagcgg ctggaaagcg atgccgtact gcgctacggc attgaagatg
    10861 tggtgacaaa tctcgacgtg ctggaacgta tgcagccttc cgaaagcctg ctacgcgccg
    10921 ttttgcacac caaacatctg atgaaccccg aagtactggc tgccgcccgc aggattgtgc
    10981 gccaggttgt ggaagaaatt atggcgcgac tggcaaagga agttcgtcag gctttttctg
    11041 gtgtccgcga tcgtcgtcgc cgctcattta ttccactggc gcgaaacttt gatttcaaaa
    11101 gtactctgcg cgccaacctg caacactggc acccgcaaca cggcaagttg tatatcgaat
    11161 tcccccgctt taacagccgc attaaacgcc aaagcgagca atggcaactg gtcttactgg
    11221 ttgatcaaag cggatcgatg gtcgattcgg tgatccactc tgcggtgatg gcggcctgtt
    11281 tatggcagtt acccggcatt cgtacccatc tggtggcgtt tgacacaagc gtcgttgatc
    11341 tcacggcaga cgttgccgat ccggtagagt tattaatgaa agtgcaatta ggcggcggaa
    11401 ccaatatcgc cagtgccatg gagtatggtc ggcaacttat tgaacaacct gcaaaaagcg
    11461 tcattatcct cgtgagcgat ttttatgaag ggggttcatc atcattgctg acgcaccaag
    11521 tgaaaaagtg tgtccagagc ggcatcaaag tgctgggact ggcagcgctc gatagcacag
    11581 caacaccttg ctatgaccgc gatacggccc aggcgctggt taatgtcggc gcacaaatag
    11641 ccgccatgac accgggcgag ctggcatcat ggcttgcgga gaatcttcag tcatgaattc
    11701 actacgtccg gaattattag aactgacacc gcaggcactg acggcgttaa gcaatgccgg
    11761 ttttgttaag cgcagcctta aggaactgga aaatggcaac gtcccggaaa tcagccatga
    11821 gaacagcgct ttaatcgcca ccttcagtga cggtgtccgt acccagctgg cgaacggcca
    11881 ggcactgaaa gaggctcagt gcacctatgg ggccagcggc atgtgccgtc atcgcgtgat
    11941 gctggtgtta agttatcaac gactttgtgc taccgcccag cctacgggaa aagaagagga
    12001 atgggatccg gcaatctggc tggaagaact ggctaccctt cccgatgcca cccgcaaacg
    12061 cgcacaggcg ctggtcgcta aaagcatcac cattgagttg ttctgtgcgc cgggtgaaat
    12121 tccctctgcc cgcttaccga tgagcgatgt gcgtttttat tcccgcagca gtattcgttt
    12181 cgcccgttgt gattgtattg aaggcacact ttgcgaacat gtcgtactgg cagtacaggc
    12241 ctttgtccag gcaaaagcgc agcaagcgga attgactcat ttaatctggc agatgcgcag
    12301 cgaacacgtc acatcatctg acgatccgtt tgccagcgaa gaaggcaaaa cgtgtcgtca
    12361 atatgttcaa caactaagcc aggcattatg gctgagcggc atcagccagc cgcttattca
    12421 ctacgaggcc gctttcagcc gcgcgcagca agcagcggaa cgctgcagct ggcgatgggt
    12481 gagtgaatcg ctacggcaac tgcgcgccag cgttgatgcc ttccacgccc gcgccagcca
    12541 ctatcatgcc ggagaatgct tacgtcagct tgcggcatta aacagtcgat taaattgcgc
    12601 acaagagatg gcccggagcg acagtgttgg tgaagttcct cctgtgccgt ggcgcacggt
    12661 cgttggctct ggcattgccg gagaagcaaa gcttgatcat ctgcggctgg tgtctttagg
    12721 tatgcgttgc tggcaggata ttgagcatta tggtttacgc atctggttta ccgatcccga
    12781 caccggcagt attttgcacc tttcgcgcag ttggccgcga agtgaacagg aagactcacc
    12841 ggcagctacg cgtcggctgt ttagttttca ggctggcgca ctggcgggtg ggcaaattgt
    12901 ttcacaagca gcaaaacgca gtgccgatgg cgagctgctg ttagctaccc gcaaccgctt
    12961 aagcagcgtt gtaccgctgt cgcctgatgc ctggcaaatg ttgagcgcgc cgttacgcca
    13021 gccgggcatt gtggctttgc gggaatattt acgccagcgt ccccccgcct gcatacggcc
    13081 tcttaatcag gtcgataact tatttattct gccggtcgct gagtgtattt cgctcggttg
    13141 ggatagcagc cgccagacgc tggatgcgca ggtaatcagc ggcgaaggtg aagataatct
    13201 gctgacgtta tcactgccag cctcagccag cgcgccttat gccattgaac gcatggcggc
    13261 gcttttgcaa caaacagacg accccgtgtg tctggtttct ggctttgtca gttttgttga
    13321 tgggcaattg acactggaac cacaggtaat gatgacaaag actcgtg
//
LOCUS       DAJTQQ010000096        13239 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000096 DAJTQQ010000000
VERSION     DAJTQQ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13239
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.096"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(17..999)
                     /gene="kdgT"
                     /locus_tag="OO309_003209"
                     /pseudo
     CDS             complement(17..999)
                     /gene="kdgT"
                     /locus_tag="OO309_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
     gene            complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="OO309_003210"
     CDS             complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="OO309_003210"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR5848252.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2164..3198
                     /gene="rhaT"
                     /locus_tag="OO309_003211"
                     /pseudo
     CDS             2164..3198
                     /gene="rhaT"
                     /locus_tag="OO309_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="OO309_003212"
     CDS             complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="OO309_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR5848253.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="OO309_003213"
     CDS             complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="OO309_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR5848254.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5241..6710
                     /gene="rhaB"
                     /locus_tag="OO309_003214"
     CDS             5241..6710
                     /gene="rhaB"
                     /locus_tag="OO309_003214"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR5848255.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6707..7966
                     /gene="rhaA"
                     /locus_tag="OO309_003215"
     CDS             6707..7966
                     /gene="rhaA"
                     /locus_tag="OO309_003215"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR5848256.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QHHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8208..9032
                     /gene="rhaD"
                     /locus_tag="OO309_003216"
                     /pseudo
     CDS             8208..9032
                     /gene="rhaD"
                     /locus_tag="OO309_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9042..9356
                     /gene="rhaM"
                     /locus_tag="OO309_003217"
     CDS             9042..9356
                     /gene="rhaM"
                     /locus_tag="OO309_003217"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR5848257.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9657..10103
                     /locus_tag="OO309_003218"
     CDS             9657..10103
                     /locus_tag="OO309_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR5848258.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            10114..11565
                     /locus_tag="OO309_003219"
     CDS             10114..11565
                     /locus_tag="OO309_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR5848259.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11555..12625
                     /locus_tag="OO309_003220"
     CDS             11555..12625
                     /locus_tag="OO309_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5848260.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12625..>13239
                     /locus_tag="OO309_003221"
     CDS             12625..>13239
                     /locus_tag="OO309_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR5848261.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDE"
BASE COUNT         3157 a         3455 c         3488 g         3139 t
ORIGIN      
        1 gattaaggaa gcaggtttat ttcaccgtac ctaaaatttc gattttcgct gctctggctt
       61 tgactttacg tgaccagata gaggtaagaa ttggcaccag aatcgaagtc acaatgaccg
      121 ccgtcgctac cagcgaagtt gctgccggag ccatcggttt aaacgcaggc accatttctg
      181 caatcagcac aggtgtcgct accgcggccc ctgcggaact ggaagcggca attccggcag
      241 tgccatcgcc accgccaatc aatttatcgg cgataatcaa cggaataccg gtcacgataa
      301 ttactgccac acccaacagg atccccagca aaccagtctg ggcaatcaca gtcaaatcaa
      361 tggtattgcc cagcgcgaag gcaaagaatg gaatcagcgt ttgcaccgct ttgctgaaaa
      421 attctcgtaa ttcagggtca agattcccca gggcaaagcc caccaggaac ggtaatactg
      481 cgccgacgaa aacatgcggt tcaaacgagg caatcccggc agtgcccaga ataatcatcg
      541 tcatgagcgg cccggactcc aacgacatca acacaaatgc ccagcttctt ctttcgtacc
      601 gtactgctgc atgatggaag cgtaaagtcc gccgttggtc atatccatcg ccgccaccag
      661 cgccagcgtt gaaagtccgg caaagaatcc aacttcaaca ccatgttccg gaataatgcg
      721 cgaggcaatt gccgcaacca cccacgcgac ggcaatttta gttaccacca gcgtaccgga
      781 tttacgcagt accgttcccg tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca
      841 caccgccaga atgggcaccg taccggtaat catcccgttg gtgaatgatc caaaatattt
      901 ccccgccccc ggcgagaagg tgtggcacag tgcgccaagg aatagcggga cgagcatcat
      961 cccccccggg attttctcaa tcgagcgttt tatctgcatt tccatcacct gtcggcatat
     1021 ttttaagggc tgatgcgtaa ttcctcaatg gagcatcagt gttgatggaa acgaagatag
     1081 acattcattt aatcaataaa agtgatcaaa atcacatcaa ttgaaacgct gttttatttt
     1141 tataggacca ttgtttttat tttggaattg atcacaaaaa aaccaccgcc gttggcgatg
     1201 gttcattttt taagctgata tgcggcctat acgcctcatt gcagcaggcg gcaaataatt
     1261 attttttcgc cgcaaaacgt gccgctgctt cgtcccagtt caccacgttc cagaactctt
     1321 tgatgtagtc cggacggcgg ttctggaatt tcaggtagta agcatgttcc cacacatcca
     1381 ggcccagaat cgggaagccg gaagcgccag aaatagcttc acccatcagc ggagaatcct
     1441 ggttagcagt agaaaccacc gccagtttat cgcctttcag caccagccat gcccagccgg
     1501 aaccaaagcg ggaagctgcc gctttttcaa attctgcttt gaagttatca acggagccga
     1561 agtcacgttc gatagccgct ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt
     1621 tccagaacag gctgtggtta gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt
     1681 ctgctggcag ctggtccagt ttggtgatca gctcttcaac cggcaggttg gcaaattctg
     1741 gcaggctttc cagcgccgcg ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt
     1801 ggatttccat ggtctgctta tcgaagtgcg gttccagggc atcgtaagca tacggcaggg
     1861 atggcagggt atagctcata ttcatctcca gtattgtcgg gcggccgatt gttaatgccg
     1921 cgtaagcagt tggttcatta tagttaatta aatgatattg aaaatgatta tcaatgccgt
     1981 acttttcgta agggtatggt tttgcaggaa aatgcccgag atgtgaagca aatcacccac
     2041 ttaatgccgt gattgccagt aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca
     2101 gcacgatttt tacactcatc tcgtcggaga tgtgaagcga cgaaaaatga tgaggataag
     2161 aagatgagta acgcgattac gatggggata ttttggcatt tgatcggcgc ggccagtgca
     2221 gcctgttttt acgctccgtt caaaaaagta aaaaaatggt catgggaaac catgtggtca
     2281 gtcggtggga ttgtttcgtg gattattctg ccgtgggcca tcagcgccct gttactaccg
     2341 gatttctggg cgtattacag ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc
     2401 ggcgctatgt gggggatcgg taatatcaac tatggcctga ccatgcgtta tctcggcatg
     2461 tcgatgggaa ttggcatcgc cattggcatt acgttgattg tcggtacgct gatgacgcca
     2521 attatcaacg gcaatttcga tgtgctgatt aacaccgaag gcggacgcat gacgttactc
     2581 ggcgttctgg tggcgctgat tggcgtaggg attgtaactc gagccgggca gttgaaagag
     2641 cgcaagatgg gcattaaagc cgaagagttc aatctgaaaa aagggctggt gctggcggtg
     2701 atgtgcggca ttttctctgc cgggatgtcc tttgcgatga acgccgcaaa accgatgcat
     2761 gaagccgctg ccgcacttgg cgtcgatcca ctgtatgttg ctctgccaag ctatgttgtc
     2821 atcatgggcg gcggcgcgat cattaacctc ggtttctgtt ttattcgtct ggcaaaagtg
     2881 aaggatttgt cgctaaaagc cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg
     2941 ttactctcgg cactgggcgg gttgatgtgg tatctgcaat tctttttcta tgcctagggc
     3001 cacgcccgca ttccggcgca gtatgactac atcagttgga tgctgcatat gagtttctat
     3061 gtattgtgcg gcggtatcgt cgggctggtg ctgaaagagt ggaacaatac aggacgccgt
     3121 ccggtaacgg tgttgagcct cggttgtgtg gtgattattg tcgccgctaa catcgtcggc
     3181 atcggcatgg cgaattaatc tttctgcgaa ttgagatgac gccactggct gggcgtcatc
     3241 ccggtttccc gggtaaacac caccgaaaaa tagttactat cttcaaagcc acattcggtc
     3301 gaaatatcac tgattaacag gcggctatgc tggagaagat attgcgcatg acacactctg
     3361 acctgccgca gatattgatt gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa
     3421 acgcgctcac tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg acttttcagg
     3481 ctagccgcca gccgggtaat cagcttatcc agcaacgttt cgctggatgt tggcggcaac
     3541 gaatcactgg tgtaacgatg gcgattcagc aacatcacca actgcccgaa cagcaactca
     3601 gccatttcgt tagcaaacgg cacatgctga ctactttcat gctcaagctg accgataacc
     3661 tgccgcgcct gcgccatccc cacgctacct aagcgccagt gtggttgccc tgcgctggcg
     3721 ctaaatcccg gaatcgctcc ctgccagtca agattcagct tcagccgctc cgggcaataa
     3781 ataatattct gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc gtcagcatga
     3841 atataaaaga gatcgccacg ggtaatgcga taaggacgat cgttgagtac atgcagacca
     3901 ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg tatgttcggc aaagacatct
     3961 tgcggataac ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg
     4021 agaagtttta actgatgcgc caccgtggct acctcggcca gagaacgaag ttgattattc
     4081 gcaatatggc gtacaaatac gttgagaaga ttcgcgttat tgcagaaagc catcccgtcc
     4141 ctggcgaata tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt
     4201 actgtcgctg aatccacagc gataggcgat gtcagtgacg ctggcctcgc tgtggcgtag
     4261 tagatgtcgg gctttcatca gtcgcaggcg gttcaggtat cgctgaggcg tcagtcccgt
     4321 ttgctgctta agctgccgat gtagcgtacg cagtgaaaga gaaaattgat ccgccacggc
     4381 atcccaattc acctcatcgg caaaatggtc ctccagccag gccagaagca agttgagacg
     4441 tgatgcgctg ttttccaggt tctcctgcaa actgctttta cgcagcaaga gcagtaattg
     4501 cataaacaag atctcgcgac tggcggtcga gggtaaatca ttttcccctt cctgctgttc
     4561 catctgtgca accagctgtc gcacctgctg caatacgctg tggttaacgc gccagtgaga
     4621 cggatactgc ccatccagct cttgtggcag caactgattc agcccggcga gaaactgaaa
     4681 tcgatccggc gagcgataca gcacattggt cagacacaga ttatcggtat gttcatacag
     4741 atgccgatca tgatcgcgta cgaaacagac cgtgccaccg gtgatggtat agggctgccc
     4801 attaaacaca tgaatacccg tgccatgttc gacaatcaca atttcatgaa aatcatgatg
     4861 atgttcagga aaatccgcct gcgggagccg gggttctatc gccacggacg cgttacaaga
     4921 cggaaaaaaa tccacactat gtaatacggt catactggcc tcctgatgtc gtcaacacgg
     4981 cgaaatagta atcacgaggt caggttctta ccttaaattt tcgacggaaa accacgtaaa
     5041 aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag catcacatca
     5101 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
     5161 attttcctgt cagtaacgag aaggtcgcga attcaggcgc tttttagact ggtcgtaatg
     5221 aaattcagca ggatcacatt atgacctttc gcaattgtgt cgccgtcgat ctcggcgcat
     5281 ccagtgggcg cgtgatgctg gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg
     5341 aaatccatcg ttttaacaat gggctgcata gccaaaacgg ttatgtcacc tgggatgtgg
     5401 atagcctgga aagtgccatt cgccttggat taaacaaggt gtgcgaggaa gggattcgta
     5461 tcgatagcat tgggattgat acctggggcg tggactttgt gctgctcgac caacagggtc
     5521 agcgtgtggg cctgcccgtt gcttatcgcg atagccgcac caatggcctg atgccgcagg
     5581 cacaacagca actcggcaaa cgcgatattt atcagcgtag cggcatccag tttctaccct
     5641 tcaatacgct ttatcagttg cgtgcgctga cggagcaaca acctgaactt attccacaca
     5701 ttgctcacgc tctgctgatg ccggattact tcagttatcg cctgaccggc aagatgaact
     5761 gggaatatac caatgccacc accacacaac tggtcaatat caatagcgac gactgggacg
     5821 agtcgctact ggcgtggagc ggggccaaca aagcctggtt tggtcgcccg acgcatccgg
     5881 gtaatgtcat tgggcactgg atttgcccgc agggtaatga gattccggtg gtcgccgttg
     5941 ccagccatga taccgccagc gcggttatcg cctcgccgtt aaacggttca cgcgccgctt
     6001 atctctcttc tggcacctgg tcattgatgg gcttcgaaag ccagacgcca tttaccaatg
     6061 acacggcgct ggcagccaac atcaccaatg aaggcggggc ggaaggtcgc tatcgggtgc
     6121 tgaaaaatat tatgggctta tggctgcttc agcgagtgct tcaggaacgg caaatcaacg
     6181 atctcccggc gcttatcgcc gcgacacagg cacttccggc ctgccgcttc atcatcaatc
     6241 ccaatgacga tcgctttatt aatcctgacg agatgtgcag cgaaattcag gctgcgtgtc
     6301 gggaaacggc gcaaccgatc ccgggaagtg atgctgaact ggcgcgctgt attttcgaca
     6361 gtctggcgct gctgtatgcc gatgtgttgc atgagctggc gcagctgcgc ggtgaagatt
     6421 tctcgcaact gcatattgtc ggcggcggct gccagaacac gctgctcaac cagctatgcg
     6481 ccgatgcctg cggtattcgg gtgatcgccg ggcctgttga agcctcaacg ctcggcaata
     6541 tcggcatcga gttaatgacg ctggatgaac tcaacaatgt ggatgatttc cgtcaggtcg
     6601 tcagcaccac cgcgaatctg accaccttta cccctaatcc tgacagtgaa attgcccact
     6661 atgtggcgca gattcactct acacgacaga caaaggagct ttgcgcatga ccactcaact
     6721 ggaacaggcc tgggagctgg cgaaacagcg tttcgcggcg gtggggattg atgtcgagga
     6781 ggcgctgcgc caacttgatc gtttacccgt ttcaatgcac tgctggcagg gcgatgatgt
     6841 ttccggtttt gaaaacccgg aaggttcgct gaccgggggg attcaggcca caggcaatta
     6901 tccgggcaaa gcgcgtaatg ccagtgagct acgtgccgat ctggaacagg ctatgcggct
     6961 gattccgggg ccgaaacggc ttaatttaca tgccatctat ctggaatcag atacgccagt
     7021 ctcgcgcgac cagatcaaac cagagcactt caaaaactgg gttgaatggg cgaaagccaa
     7081 tcagctcggt ctggatttta acccctcctg cttttcgcat ccgctaagcg ccgatggctt
     7141 tacgctctcg catcccgacg acagcattcg ccagttctgg attgatcact gcaaagccag
     7201 ccgtcgcgtt tcggcctatt ttggcgagca actcggcaca ccatcggtga tgaacatctg
     7261 gatcccggat ggtatgaaag atatcaccgt tgaccgtctc gccccgcgtc agcgtctgct
     7321 ggcagcactg gatgaggtga tcagcgagaa gctaaaccct gcgcaccata tcgacgccgt
     7381 tgagagcaaa ttgtttggca ttggcgcaga gagctacacg gttggctcca atgagtttta
     7441 catggggtat gccaccagcc gccagactgc gctgtgcctg gacgccgggc acttccaccc
     7501 gactgaagtg atttccgaca agatttccgc cgccatgctg tatgtaccgc agttgctgct
     7561 gcacgtcagc cgtccggttc gctgggacag cgatcacgta gtgctgctgg atgatgaaac
     7621 ccaggcaatt gccagtgaga ttgtgcgtca cgatctgttt gaccgggtgc atatcggcct
     7681 tgacttcttc gatgcctcta tcaaccgcat tgccgcgtgg gtcattggta cacgcaatat
     7741 gaaaaaagcc ctgctgcgtg cgttgctgga acctaccgct gagctgcgca agctggaagc
     7801 ggcgggcgat tacactgcgc gtctggcact gctggaagag cagaaatcgt tgccgcggca
     7861 ggcggtctgg gaaatgtatt gccaacatca cgatacgcca gcaggtagcg aatggctgga
     7921 gagcgtgcgg gcttatgaga aagcgatttt gagccagcgt gggtaaacac tgccggatgc
     7981 ggcacgagcg ccttatccgg cctacgggtc gaatgtccat gcgggctgtc accctcaccc
     8041 cggccctctc cctgaaaggg cgagggagaa aaacatagcc tcgtgcggca acggagctga
     8101 acaccgctcc ggacagtccc ctcgccccta cggggagagg gttagggtga ggggaacaag
     8161 ccgcaccatg tctaaacaag gcaacctaat ttacaggaac acagaacatg caaaacatta
     8221 cccagtcctg gtttgtccag ggaatgatca aagccaccac cgatgcctag ctgaaaggct
     8281 gggacgagcg caacggcggc aacctgacgc tgcgcctgga tgacgccgat atcgcgcttt
     8341 atcacgacaa cttccaccca caaccgcgct atattccgct cagccaaccc atgcctttac
     8401 tggcaaatac gccgtttatt gtcaccggct ccggcaaatt cttccgtaac gtccagctcg
     8461 atcctgcagc taacttaggc gtcgtaaaag tcgacagcga tggcgcgggc taccatattc
     8521 tctgggggtt aaccaacgaa gccgttccaa cttctgaact tccggctcac tttctctccc
     8581 actgcgagcg cattaaagcc accaacggta aagatcgggt gatcatgcac tgccacgcca
     8641 ccaacctgat cgccctcacc tatgtactgg aaaacgacac cgcggtcttt actcgccaac
     8701 tgtgggaagg cagcaccgaa tgtctggtag tattcccgga tggcgttggc attctgccgt
     8761 ggatggtgcc cggcacggac gaaatcggcc aggcgaccgc acaagagatg caaaaacatt
     8821 cgctggtgtt gtggcccttc cacggtgtgt tcggttccgg accgacgctg gatgaagcct
     8881 ttggtttaat cgacaccgca gaaaaatcag cacaagtatt agtgaaggtt tattcgatgg
     8941 gcggcatgaa acagaccatc agccgtgaag agttgatagc gctcggcaag cgcttcggcg
     9001 ttacaccact agccagtgcg ctggcgctgt aaggagcaaa catgatccgc aaagcctttg
     9061 tcatgcaggt aaaccccgac gcccacgaag agtatcagcg tcggcataat cccatctggc
     9121 cggaactgga agcagtgttg aaatctcacg gtgtgcataa ctacgccatt tacctcgaca
     9181 aagcgcgtaa tctgctgttt gccatggtag agattgaatc tgaagaacgg tggaatgcgg
     9241 tagccagtac tgatgtttgc caacgttggt ggaaatatat gaccgatgtt atgcccgcta
     9301 acccagataa cagcccggtg agtagcgagc tgcaagaagt gttttacctg ccgtaattgc
     9361 ccaatgtgcc ggagatgcct ccggcacaat attttaaatt tcgatttgaa aaattaaaca
     9421 tattacatct cccggtaagt tatatttccc tgatacattg tgagtaaatc acaaaaataa
     9481 tgaataaccc attaatgatt catgtggttt atttaaataa ccattatgtg cattactccg
     9541 caaatctgac ctttcactct gtctcaaatt gtcattgttt accgacatat cgcctctcat
     9601 cacttaatcg ctccaccatt acaattgatt aaaatatatt ttatggagtc cgattaatgg
     9661 cagctcttac tgcaagctgt attgacctga atattcaggg caatggcgct tattccgttc
     9721 tgaagcagtt ggcgacaata gcgttacaaa acggttttat caccgactca caccagttcc
     9781 tgcaaaccct gctgctgcgc gaaaaaatgc actctactgg atttggttcc ggtgtcgcag
     9841 tgccgcacgg taaaagcgcc tgcgttaaac aaccgttcgt attattcgcc cgcaaagcgc
     9901 aggctattga ctggaaagcc agcgatggcg aagacgtcaa ttgctggatc tgcctcggcg
     9961 tgccgcaaag cggcgaagag gatcaggtca aaatcatcgg cacactgtgt cgcaaaatta
    10021 ttcaccagga atttattcat caactgcaac agggcgatac cgaccaggtg ctggccttgt
    10081 taaatcaaac cctcagctca taaggaagtg gcgatggagt catccttacg tattgtcgcg
    10141 atcaccaact gccccgccgg gatcgctcac acctacatgg tggcggaagc cctggaacag
    10201 aaagcccgtt ctctcggcca taccataaaa gtggaaactc aagggtccag tggcgttgaa
    10261 aaccgcttat ccagcgaaga gattgccgct gccgattacg tcattcttgc taccgggcgt
    10321 ggcctgagcg gtgatgatcg cgcgcggttt gccgggaaga aagtttatga gattgccatc
    10381 tccctggcgt tgaaaaatat cgaccagatt ttcagcgaat taccgacaaa ctcgcagctt
    10441 tttgccgcag atagcggcgt gaagcttggc aaacaggaag tgcaaagcgg cagcgtgatg
    10501 agccacctga tggcgggcgt ctctgcggcg ctgccgtttg tcatcggcgg tggtatcctg
    10561 gtggcgcttg ccaacatgct ggtgcagttc ggtttgccgt ataccgatat gtcgaaaggc
    10621 gcgccgtcgt ttacctgggt agttgaatcc atcggttacc ttggcttcac ctttatgatc
    10681 cccatcatgg gcgcttatat tgcctcgtcg attgccgata aaccggcttt cgctccggcg
    10741 tttctggttt gctatctggc gaacgacaaa gcgctgctcg gcacccagtc gggtgcggga
    10801 ttcctcggcg cggtggtgct ggggctggcg attggctatt tcgtcttctg gttccgcaaa
    10861 gtgcgccttg gcaaagcatt acaaccgctg ctcggttcaa tgctgatccc gttcgttact
    10921 ctgctggttt tcggcgtgct cacttactac gttatcgggc cggtgatgtc cgacctcatg
    10981 ggcgggctgc tccacttcct gaataccatt cctccatcaa tgaagtttgc ggcagcgttt
    11041 ctggtcggtg cgatgctggc gttcgatatg ggcggcccca tcaataaaac cgcctggttc
    11101 ttctgcttct cactgctgga aaaacacatt tatgactggt acgccatcgt cggcgttgtc
    11161 gcgttgatgc cgcccgttgc ggcgggtctt gcgacgttca ttgcgcccaa actgtttacc
    11221 cgacaggaaa aagaggctgc cagtagtgcc attgtggtcg gtgccacagt cgcaaccgaa
    11281 ccggctatac cttacgctct ggcagctccg ttgccgatga ttaccgccaa tacgctggcg
    11341 ggcggcatta ccggcgtgct ggtgatcgct ttcggaatca aacgtctggc acctggtttg
    11401 ggtatctttg acccgctgat cggcctgatg tcgccggtag gttcgtttta tctggtgctg
    11461 gcgattggcc tcgcgctgaa catttcattc attatcgtcc tgaaaggatt gtggttacgc
    11521 cgcaaagcga aagccgcgca acaggagctt gtccatgaac attgagttac tgcaacagtt
    11581 gtgcgaagcc agcgccgtta gcggcgatga acaggaagtt cgcgacatcc tgataaacac
    11641 gctggaacct tgcgttaatg aaatcacctt tgatggtctg ggcagctttg ttgcccgtaa
    11701 gggaaataaa ggtccaaaag ttgccgttgt cgggcatatg gatgaagtcg gctttatggt
    11761 cacccacatc gacgagagcg gttttctgcg tttcaccacc attggtggct ggtggaatca
    11821 gtcgatgctc aaccatcggg taaccatacg cacacacaag ggagtgaaaa tccctggtgt
    11881 gattggtttc gtcgcgcctc atgcgttaac ggaaaagcaa aggcaacagc cgctgtcatt
    11941 tgatgagatg ttcattgata ttggcgcaaa cagtcgcgat gaggtggaaa aacgcggtgt
    12001 ggcgatgggc gattttatta gcccggaagc caattttgcc tgctggggcg aagataaagt
    12061 ggtcggcaag gcgctggata accgcatcgg ctgcgcaatg atggctgaac tattgcagac
    12121 ggtgaataat cccgaaatta cgctgtatgg cgtcggcagc gtggaagaag aagttgggct
    12181 acgcggggca caaacctcgg cggaacacat taaaccggat gtagtgatcg tgctggatac
    12241 cgctgtcgca ggcgatgttc cgggcattga taacattaaa tacccgctga aactgggcca
    12301 ggggccgggg ctgatgctgt ttgacaagcg ctacttcccc aaccagaaac tggtagcagc
    12361 gttaaaaaac tgtgccgcac ataacgattt accgctgcaa ttttccacca tgaaaaccgg
    12421 agcgacggat ggcgggcgct acaacgtgat gggcggcggg cgtccggttg ttgcgctgtg
    12481 cctgccaacc cgctatctgc acgctaacag cggcatgatt tcaaaagccg attacgatgc
    12541 tctgctcacg ttgatacggg gttttctgac gaccttaacc gcggagaaag tcaacgcgtt
    12601 tagccagttc cgtcaggtgg attaatgcta aacgaacgcc agttaaagat tgtcgatctg
    12661 ctggagcaac agccgcgcac gcctggtgag ctggcgcaac agactggcgt ttcaggcagg
    12721 accatcctgc gtgatattga ctatctcaac tttaccctta acggcaaagc ccgcattttc
    12781 gccagtggca gtgcgggcta tcagctggaa atcttcgagc gccgcagctt ttttcagttg
    12841 ctgcaaaagc acgataacga cgatcggctg ctggcgctgt tattactgaa tactttcact
    12901 ccccgtgcgc aactcgcctc ggcgcttaat ttgccagaaa cgtgggtagc agagcgtctg
    12961 ccccagttaa aacagcgtta tgaacgcact tgttgcctgg ccagccgccc tggtttgggc
    13021 catttcattg atgagacaga agagaaactc gttatcttgc tggcgaactt gctgcgcaaa
    13081 gatccgtttt taattccgct ggcgggcata acacgagaca accttcagca tttatccacg
    13141 gcctgcgaca accaacaccg ctggccgctc atacagggtg attatctctc cagcctgatt
    13201 ctggcgattt acgccctgcg taatcaactg accgatgag
//
LOCUS       DAJTQQ010000097        13087 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000097 DAJTQQ010000000
VERSION     DAJTQQ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13087
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.097"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..890)
                     /locus_tag="OO309_003222"
     CDS             complement(<1..890)
                     /locus_tag="OO309_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5848262.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAP"
     gene            1131..1931
                     /locus_tag="OO309_003223"
     CDS             1131..1931
                     /locus_tag="OO309_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5848263.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2009..2779
                     /locus_tag="OO309_003224"
     CDS             2009..2779
                     /locus_tag="OO309_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5848264.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="OO309_003225"
     CDS             complement(2827..4173)
                     /gene="mltD"
                     /locus_tag="OO309_003225"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR5848265.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="OO309_003226"
     CDS             complement(4245..5000)
                     /gene="gloB"
                     /locus_tag="OO309_003226"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR5848266.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5034..5756
                     /locus_tag="OO309_003227"
     CDS             5034..5756
                     /locus_tag="OO309_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5848267.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="OO309_003228"
     CDS             complement(5753..6220)
                     /gene="rnhA"
                     /locus_tag="OO309_003228"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR5848268.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6285..7016
                     /gene="dnaQ"
                     /locus_tag="OO309_003229"
     CDS             6285..7016
                     /gene="dnaQ"
                     /locus_tag="OO309_003229"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR5848269.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7149..7225
                     /locus_tag="OO309_003230"
     tRNA            7149..7225
                     /locus_tag="OO309_003230"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7183..7185,aa:Asp,seq:gtc)
     gene            complement(7356..8402)
                     /locus_tag="OO309_003231"
     CDS             complement(7356..8402)
                     /locus_tag="OO309_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCR5848270.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="OO309_003232"
                     /pseudo
     CDS             complement(8416..8975)
                     /gene="tssG"
                     /locus_tag="OO309_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="OO309_003233"
     CDS             complement(8972..10855)
                     /gene="tssF"
                     /locus_tag="OO309_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR5848271.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="OO309_003234"
     CDS             complement(10871..11365)
                     /gene="tssE"
                     /locus_tag="OO309_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR5848272.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11362..12192)
                     /locus_tag="OO309_003235"
                     /pseudo
     CDS             complement(11362..12192)
                     /locus_tag="OO309_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12179..13081)
                     /gene="tagK"
                     /locus_tag="OO309_003236"
                     /pseudo
     CDS             complement(12179..13081)
                     /gene="tagK"
                     /locus_tag="OO309_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596542.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3006 a         3392 c         3482 g         3207 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg
      841 ctttcgacga ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct
      901 gttgcaaaat agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat
      961 atgtcatccg atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc
     1021 ttacataaca atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga
     1081 accctcccga gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa
     1141 acacctatgc catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt
     1201 cttttgcgag catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta
     1261 ttcggatcct ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa
     1321 agaactacgg caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt
     1381 tagtacagtt tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc
     1441 cacaacatcc ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt
     1501 tacgtgaacg agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat
     1561 tacctgacac ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg
     1621 tggatgtcta tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc
     1681 cggtcattat ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc
     1741 gctttgcgcg ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg
     1801 cgtttggccg cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg
     1861 tattggttac gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc
     1921 ctgataaata aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt
     1981 tatactcaaa caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga
     2041 aaagcagttt agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg
     2101 agatttacag cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat
     2161 gggctgcgga gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc
     2221 gtatgactta tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact
     2281 gaaaaatatc accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt
     2341 tgatattgtt atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg
     2401 tgaagtgaac cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc
     2461 gggtcatcca gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca
     2521 cgtacgaaac tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt
     2581 tgataattta attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg
     2641 tacgccagaa gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt
     2701 gagaacgtat tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga
     2761 agcacataaa gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct
     2821 ggtttgtcag gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg
     2881 ttgcagattc gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg
     2941 tttagcaatg cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt
     3001 gtttgccagt cgctgtgcac caatcgtcaa actttggcct ggcttcagct tagatccgcg
     3061 cagtttgttc cactgctgca aatctttggt gcttacgccg agacgtgaag cgatgcttga
     3121 aagcgtgtcg ccagagcgta cggtgtaaac acggctgtta agcggcgtat tgttggcaac
     3181 cagcgtcgac tgtacagcag caatttcgcc tgaagcgaga gattcacgca gttgatctgc
     3241 atgcttcttt ggcaccatca cgtactgcgg cccacttgcg cccagcgtgg agcctttcac
     3301 gccagcgttg aatgtcttca gcttgctgac ggaaatcccc gccatatctg caaccttcgc
     3361 catttcaacc gggctactca ggtgcacacg cgccagagca cggctttcat cggtcgttgg
     3421 cagacgtacg ccataacgct tgctgttttt gagaatatca ctcaatgcca gcattttagg
     3481 cacgtactgc ttcgtttcct gcggcaacgg taacgaccag aagtccgtgg atttcccacg
     3541 cgctttgttc gttttaattg ccttcatgac ccgaccttcg ccgctgttat aagccgctac
     3601 ggtcagaagc cagtcgtcgt caaacatttt gttcagacgc tgcatcatat tcagcgcggc
     3661 agttgttgaa gcaacaacat cgcgacgcgc gtcataattg cgggtctgtt tcaaaccata
     3721 attgcgcccc gtgctcggaa tgatctgcca gatgcctgcg gcattggcgc cagacgttgc
     3781 gtgaggatca aaagcgctct ccactatggg tagtagtacc agttccatag gcatgttacg
     3841 ttttttaact tgccctgcta tccagtacat atacggctct gcccgtaaag ttacatcgtg
     3901 gagatagctc ttattgcgta aatatttctg tttctgttcg cgaatccggt cattttccgg
     3961 aattcccatc tttagctcgt cgccaatgaa agcccacaag tcaccatctg gcgcgataga
     4021 cgtcccatcg tccatccatc gtgcctgact tgtaaacttt gctgcttccc cttgaccagc
     4081 tgcagaaagg ctctgtgcgt gctgttgaac gttgccggta ctctggcaac ccacgagcag
     4141 gacagaggcg agtaatatcg cttttgcctt catgtgtgtg tcaatagttg cttaaaagac
     4201 gaccgatcat aacggcgaac ggagccgatg acaagaaagt tttatcagaa cctatctttc
     4261 tttgacctta accatgcaaa acgctcttca ggttgttgca ataatgtttc ttcattaatt
     4321 ccattaatta aatcaatatc ttccgttctt aaaaaaacat taatttgccg ctcatttttc
     4381 agaattacgg gtagtgttat ttgatttttt gcccgtaact ccttaacttt acgataataa
     4441 tcatttatgg acaaatcgtg cggaaaaata ctcaaagcaa acttcatatt tgataacgta
     4501 tattcatgag cacaacatac caatgtatcg tcaggtaacg cacttagctt tttaagtgat
     4561 tgatacattt gtaaagctgt cccttcaaac aaccgaccac acccaccaga aaacagcgtg
     4621 tcgccgcaaa atagataagg tttactgaag taacagatat gtcctaaagt gtgacccggt
     4681 gtggtaatta cactaaattc atgccctaaa acgaaggcag tttcgccatc tttgactacc
     4741 tgtgttgttc ccttatcttg tgtctcttgt ggaccataca ctacaatttg cggaaacttt
     4801 tccaccagtt cttttacgcc gccaacgtga tcgtggtgat ggtgggttag aaatatggcc
     4861 tccggttgcc agttattggc ggtaatggcg tttaatactg gctctgcgtc tccgggatcg
     4921 acaatcaggc agcgacctgc ctcatcattc aaaacccaga tgtaattgtc atcaaaggcg
     4981 ggaatactgt taagattcat agattacctc tcagtgtaaa acggaaggtt gtgatgaaac
     5041 cggcaagagt ccctcaaact gtcgtggctc ctgattgctg gggcgatttg ccctggggag
     5101 agctttatcg caaggcgctg gagcgccagc tcaacccgtg gttcactaaa atgtatggtt
     5161 ttcatctgct taagattggc aatttaagcg cagaaatcaa ttgcgaagcg tgcgcggttt
     5221 ctcatcaagt gaatgtttct gcgcaaggaa tgcccgtcca ggtgcaggcg gatccacttc
     5281 atcttccttt tgccgataaa tccgttgata tttgtctact ggcacatacc ttgccgtggt
     5341 gcaccgatcc gcatcgttta ttacgtgaag ccgatcgggt actgattgat gatggctggc
     5401 tggtgattag tggcttcaac cccatcagtt taatgggatt acgcaaactt gtgccggtat
     5461 tgcgcaaaac ctcgccctat aatagccgga tgtttactct gatgcggcaa ctggactggc
     5521 tctctttgtt gaattttgaa gtgctgcacg ccagccgttt ccacgttctc ccgtggaaca
     5581 aacacggagg aaaactattg aatgcgcata ttcctgcgct tggttgctta caacttattg
     5641 ttgcccggaa acggactatt cctttaacgc taaatccgat gaaacagagt aaaaacaagc
     5701 cacgaattcg ccaggcggtt ggagccaccc ggcaatgtcg taaaccacag gcttaaactt
     5761 cgacttggta gcccgtatct tccagtgtgg gattcatcgc cgcggcacga gccagttcat
     5821 cacagcgttc gttttccggg tgtccggcat ggcctttaac ccattcccat ttgatttgat
     5881 gctgccccaa tgcagcatca agacgttgcc agagatcgac attttttact ggttttttgt
     5941 ctgctgtttt ccagccacgt tttttccagt tatggatcca ctgggtgata ccctggcgga
     6001 catactggct gtcggtactc aaaatgactt cgcaatgttc ttttaacgcc tccagcgcga
     6061 caatagcggc catcaactcc atacggttgt tggtggtgcg ggtgtagcct gcgctaaagg
     6121 ttttctcgcg tccgcgatag cgtaaaatag cgccgtaacc cccaggtcca ggattaccca
     6181 gacacgaacc atcggtgaaa atttctacct gtttaagcat ctctggtaga cttcctgtaa
     6241 ttgaatcgaa ctgtaaaacg acaagtctga cataaatgac cgctatgagc actgcaatta
     6301 cacgccagat cgttctcgat accgaaacca ccggtatgaa ccagattggt gcacactatg
     6361 aaggccacaa gatcattgag attggtgctg ttgaagtagt gaaccgtcgc ctgacgggca
     6421 ataacttcca tgtttatctc aaacccgatc ggctggtgga tccggaagcc tttggcgtac
     6481 atggtattgc tgatgaattt ttgctcgata agcccacgtt tgccgaagta gccgatgagt
     6541 ttatggacta tatccgcggc gcggagttgg tgatccataa cgcagcgttc gatataggct
     6601 ttatggacta cgagttttcg ttgcttaagc gcgatattcc gaagaccaat accttctgta
     6661 aggtcaccga tagccttgcg gtggcgagga aaatgtttcc cggtaaacgc aacagcctcg
     6721 atgcgctatg tgcccgctac gaaatagata acagtaaacg aacgctgcac ggggcattac
     6781 tcgatgccca gatccttgcg gaagtttatc tggcgatgac cggtggtcaa acgtcgatgg
     6841 cttttgcgat ggaaggagag acacagcagc aacaaggtga agcaacaatt cagcgcattg
     6901 tacgtcaggc aagtaagtta cgcgttgttt ttgcgacaga tgaagagctg gcagcacatg
     6961 aagcccgtct cgatctggtg cagaaaaaag gcggaagctg cctctggcgg gcataaatac
     7021 atgtaaaagg cgctaaaaat agcgacttgg gcgatttttg cagcaaacga ttcaaaagat
     7081 gagaaaaatc gttgacgaag gtcgaggcaa tccgtaatat tcgcctcgtt cccaacggaa
     7141 cacaacgcgg agcggtagtt cagtcggtta gaatacctgc ctgtcacgca gggggtcgcg
     7201 ggttcgagtc ccgtccgttc cgccaatatt cagaaagccc gaatcagtaa tgattcgggc
     7261 tttcgtcgtt ttggtgaagt gagaattttt cactccgaag taaaacggat atgatttaaa
     7321 aaatggccag acattataaa ttctggccac ttctgttact ccagcgcatc ccatttctcc
     7381 agcagttcta ctggaatttc atttattacc tccgagacgc gttttccgac caggcgttct
     7441 gcctggcgca gcaaaggaat ggtcgggctg ctcggttcgc tgtgctcaaa ccagcgacgg
     7501 atgacacgca ggcgttccag cgcgtcgttt cggtcgcgga tctgccgggg ttctgcgccg
     7561 gtcatcggtg atatttcctc cgcgatatgc accggtagcg caatctgctc tgcctgcatc
     7621 tgttcagtat gaacgatggt ggcgttttcc ggctgctcag cctgtggttg tggcaggatc
     7681 tccggcgtgg tgctctgcac tgctcccggt aacagggcca gcagttgcgt cagacgggag
     7741 aagtccggtg cctggtcatt cagcgtttca cgcgcatggc gttgcagccg ctcagtggtc
     7801 tctgctgcct ggcgaaaggc atccagcggc agtgtgccgc gcgcttccag gtctgccagt
     7861 tgctggcgta ccgattctgg tgccagcgca tccgccgggc gtaatgcaga tagcgaacgc
     7921 tccacatccc gcacctgaag gcgcatggca gcattatttg acagtgtaat gccacgaata
     7981 tccgccatta caccttcgtg gtccagcaac gccgccagcg cgttactgcg tgccaccgca
     8041 gcatcttcgg cggtgatatc ttcagtcgcg agcagttgag gatggatggc gtccgggtac
     8101 gtggagcaca acgtttccag cagcgtgagc gcctccgaca gtcccctggc accggcctgc
     8161 tggatacggc agcgcagcag gacaaccaga atgcggatat ctttgctgcg catcagcaga
     8221 cgatgggcgt cccgctcaag ctccgcccag ttaatgtttt ccggcgtact gacaaaatcg
     8281 ccatattgcg cttcttcacg cggtgcggcg cgggtgaaca gcagcaggta ttccggatcg
     8341 tactccagat ccgggccgca ggggcattcc gccgaaattg ggtcagacag gaatatttcc
     8401 atttaattat cctcagtgcg ccagcgtggt ggccagaccg tccggattgc tggactcacg
     8461 caccagatgg gccgatgccg ccagacgagc gtgatctggc aacgggctgt cagcgctctc
     8521 ccgcaaatcc tgcccgctga ggctggcgac ataaaaggca aagcggtcat gttgcggttt
     8581 atccagaccg cccccagcgg actgggcgct gcgccatgcc tggtaaaact gcgtcagcca
     8641 gcggtgatga aaaagatcag aaaaacgggt cagcgtcggc tttcagtccg gttacgcgcc
     8701 agttcggtgt agtgcagcgg cagtggaccg ttcggccccc acagcacgag gctgtacaga
     8761 ctgatgtgca ggcgcccttc ctgccagccc acactggcga tttctcgtgg cgcaaaggtc
     8821 agcgcgggcg tttgtccgag gcgaaatttt tccatccgtg gttgccaggt gttgcccgcc
     8881 ggaacggtac acaactgcac atccacgcgg cgcatcaggt tcagaaaacc gtaacgccag
     8941 gggggggcct gcgcctgagt gaaatcgttg ctcatacgct tccccgctgt cccgccctga
     9001 caggccaggt cataattttg ccgcgctgca tgctgtgcag cgtcatctgc gagaaggtat
     9061 taatggaaac atggcgggcg atatagtgtt ccagcaccag gccgaacaga tacggactga
     9121 tgccggaaaa accttcttca tccaccgtca gttcacaact gacgccacgc ccatagacca
     9181 gtagtccgga accgggcaga cggcgggtga ccggggtcgt tttacaacca atcaggctgc
     9241 gcacctggcg cgattgcgga ctgtcatgag caggaataaa caggttcagc agatctcgca
     9301 acgcctggcc gccggtacgg tgatccagat cggccagcgg cagataatta aaagataact
     9361 ggcggatcaa ccgccatgcc atctcacgtt cagccatcgg gggctgcggc gagcgtggcg
     9421 ggcggataag ccctacgccc gccaccggga tggctgcatc cacggttaga tcgtcccggc
     9481 cattgcgggc aataagacag ggcagatcgc ggttagtgac catggccgtg atggtgatgt
     9541 ggcgcagatt ttccgggtac ggcgcttcat actggtcaac cagcgacagg aacacttccg
     9601 agcccgtata gggggtacgg gtgccgtaac ggcgggcgtt ttctgataag cggcggggct
     9661 cccggcgcag ggaaaaataa cgtccgtgat tgccttcatc attattgcgg gtgtggtaga
     9721 gcgggcgaaa ggccatcttc cgtgtggtgt cggtttccag cccttccact tcctggaccg
     9781 agaacacctc ataatccagc ggatgggttc tgtccaccac cagatgctgt tccgtcgtgc
     9841 tgtgcgtgac atcgatccgg gcggtgacgc ggggaaacag gttgataacc ggggtacaga
     9901 acaggctgaa ctgtgccgca tttgtctggt ggatcagcca gtccggcggc aggcggttca
     9961 gcaggataac aatctccgcc acgccaccgt caattttctg taatccggca gacaggcctg
    10021 tcggggtgaa aaaatagaag cgttccggac aggcaaaata ttcatgcagc aggttatgac
    10081 cgtgaaacac gttccacgcc agcggcagca gcccctgatc cggctccagg ccttcgtaca
    10141 taacgggctg ttgcagattc acatccagcg cgccgtcaaa atgccccgga atgccagcca
    10201 ggggcgcgat ggcgctggta tgtagaagtt ctaacaggtg tgaggccgta cgctcctcgc
    10261 cgcagagata aaacggcagc ctgtccagtc cggcaagctg actgaacgtt agttcaccaa
    10321 aggtgcgcag ggtaatacgc agcgccccgg cgacgtgaat gttggccggc aggtagcggt
    10381 gcagggcagg catatccggt ggcgcggcgg tcagtcgcgc ctcttcgatg gcgagcggcc
    10441 acagcgttac atcctggctg ctgcgaaact ggcaggcagt gctttcgcct tccggaatgg
    10501 cggagaaaaa ggctgtgtcg cgcgggacgg taaatccttt cgccagatcg ccttcttccg
    10561 tatcgggatg caactgcgcc acgctcattg agggcgtggg tgtgacgtaa ttggggctga
    10621 ccacttccag caggcgctgg gtgaaacggg ggaattcagc gtcaattttt agctgcgtgc
    10681 gggcggtcag gaagctgaag gcctcgacca ttcgctctac atacggatcg gcaatatccg
    10741 ttccctgcat gcccagccgg gcggcgactt tgggatgacg ggcggcaaat tcggctcccg
    10801 tttcccgcag atagctcagt tcgcggttgt agtattccag tagccgtgga tccatcggtc
    10861 tctctctgaa ttaaaaaaac gtaatgcgac tctgctccat atccagcgcg ctacgtaccc
    10921 ggaatgcagt cggatacggc tgcgtgagga tctcgccgcg aatttcaaac tgtaatgtgt
    10981 tatggccact ctggcggcgc tcatcctgaa gtgggatgat ttgcagagtg gacgctttca
    11041 gccggggttc aaaccgggtg atggttcgca aaatggccct gcggatatcg tcccacttgt
    11101 gctcatacag aaagctgccc gccagtggcg gcaggccgta gttaagtacg gacgctgccg
    11161 cgtacggata acgtgcagca tcaatatcat cttcatggct gacggtgttg agcagaaatg
    11221 acaaatcccg gcggatgatc tccttaatct gaaccggact gacgctgata ccgtggtcgc
    11281 gtttctggtt tggggcgtcg tcacacaggc ggtcaaacag cgtgggcagc aggtgattgc
    11341 ctggacgctg gcgggaagcg ctcacgcgtc gcttccttca gcggtgttaa aggtacaggt
    11401 ggacaaatcc agcaggctga tatcgccgtg accggtgagc cacaccctct gacccagcgc
    11461 ctgtaccagt gtctctcccg gaccgtcctg ccagaccgtt tcccggcaca gacgcaggga
    11521 gtcggcagcc ttttcagagc cgctgtagcg gggaaacagc taggcactgt gtgtatcgcc
    11581 attgttcagg gtgacgttga cgggtttcca gatcaaatcg atcagatgcg caggccgcgg
    11641 cgatttcagc gaacagattt gtgaaaacgg cagccagata tatgctccgc ccgcgatcag
    11701 ttcgagtacc ggaccggtac gggagtcgct gtcgctaatc cagtcaaatg cgccgccatt
    11761 ccactgcccg gaagtgtctg ttatggcctc aagcgcgcgg ctgcggtgag catcagcttc
    11821 ctgcgcttca ccacgggcgt tacaggcgag ggcggtcagc agatcctcca tccaggcggg
    11881 cggcgggaga ataacaccgg gccgctgttc gccctgaaag caggcgtggc ggtatatctc
    11941 acagcggatc agctcgccaa atacctgcgc ttcgcgagta taatttgcgt ccatccgggc
    12001 gcatagctgg atctgttgca gtgcccgcga ccagtcgcct gccacgcaca ataactgaaa
    12061 caggctgtgg cggcgggagg cctgcgctgg gtgcatcctg acctgttgct ctgccatctg
    12121 aatgccctcc accacggaat aatcccgcag cagcgccgac aaggtaatcg gtagctcgtt
    12181 attgtttgtc atgatatatc tctccatgtt gtgccgggtt tatccggtaa tggctgtcaa
    12241 tgccaatgat gcgatgctct ttccgtgtca gatctggcag tacggtgtta tgcgctgttt
    12301 ccggggcgta ttccggggaa agtaaatgca ggatatcagg gggagtcttt tcgacaggcc
    12361 agatagtttc atcttcgcca gtggcatcca gtacattgag aatagcgtca atgcttaacc
    12421 caccagtgac catatcctgc aatgtctcgg cttcggtgac caggtcacgc aatgaatcca
    12481 gatcctgcga ggagggctct tcgttaaaag gaaaaggatc agcatggcga ttaagatctt
    12541 gttcgggaga taacaacgcc tcgcggtatt cctgataaag tcgggtcagg gcatttcctt
    12601 tttccgcacc ttctccggtg gaggaggctc catgaacagg aatgatatcg aatggatttt
    12661 gtgatgtcag atgctgctgc ctgaaccaat ccagatccag atattcggtt attgcttccg
    12721 acgtgatggc agtctgcgat atcgttttta ctgtgctcag gttcggagat gatggattgc
    12781 catgtacaag gcactatgaa gataaccccc attctatcag gtcgccttca ttcagacgca
    12841 tacggtgatg aggttccaca atctggtcgt ttaccgtaca gcaatattcg gtagactgat
    12901 tgataatcca ccatgcttct tcgtggcggg tcagagtgag ggcaatcttt acatcatcat
    12961 tcaccttaaa aacaggaatc cagggagagg taacggtgaa gataatttgt gttccttcag
    13021 tcgcgtaaat tgtgttatcg gcgatcccgt gcgcctgtat tttttggagt ccccacgtaa
    13081 tatttgg
//
LOCUS       DAJTQQ010000098        12886 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000098 DAJTQQ010000000
VERSION     DAJTQQ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12886
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.098"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(118..1569)
                     /gene="uxaB"
                     /locus_tag="OO309_003237"
     CDS             complement(118..1569)
                     /gene="uxaB"
                     /locus_tag="OO309_003237"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR5848273.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1796..3214)
                     /locus_tag="OO309_003238"
     CDS             complement(1796..3214)
                     /locus_tag="OO309_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR5848274.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3368..3712)
                     /locus_tag="OO309_003239"
     CDS             complement(3368..3712)
                     /locus_tag="OO309_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR5848275.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3712..4638)
                     /gene="glsB"
                     /locus_tag="OO309_003240"
     CDS             complement(3712..4638)
                     /gene="glsB"
                     /locus_tag="OO309_003240"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR5848276.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4702..5637)
                     /locus_tag="OO309_003241"
     CDS             complement(4702..5637)
                     /locus_tag="OO309_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR5848277.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5717..6832
                     /gene="yneK"
                     /locus_tag="OO309_003242"
     CDS             5717..6832
                     /gene="yneK"
                     /locus_tag="OO309_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR5848278.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6982..8172
                     /gene="ydeA"
                     /locus_tag="OO309_003243"
     CDS             6982..8172
                     /gene="ydeA"
                     /locus_tag="OO309_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR5848279.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8197..8862)
                     /gene="marC"
                     /locus_tag="OO309_003244"
     CDS             complement(8197..8862)
                     /gene="marC"
                     /locus_tag="OO309_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR5848280.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9074..9508
                     /gene="marR"
                     /locus_tag="OO309_003245"
     CDS             9074..9508
                     /gene="marR"
                     /locus_tag="OO309_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR5848281.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9528..9911
                     /gene="marA"
                     /locus_tag="OO309_003246"
     CDS             9528..9911
                     /gene="marA"
                     /locus_tag="OO309_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR5848282.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9943..10161
                     /gene="marB"
                     /locus_tag="OO309_003247"
     CDS             9943..10161
                     /gene="marB"
                     /locus_tag="OO309_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR5848283.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10192..11091)
                     /gene="eamA"
                     /locus_tag="OO309_003248"
     CDS             complement(10192..11091)
                     /gene="eamA"
                     /locus_tag="OO309_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR5848284.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11355..12473
                     /gene="ydeE"
                     /locus_tag="OO309_003249"
     CDS             11355..12473
                     /gene="ydeE"
                     /locus_tag="OO309_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR5848285.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12600..12695
                     /gene="mgtS"
                     /locus_tag="OO309_003250"
     CDS             12600..12695
                     /gene="mgtS"
                     /locus_tag="OO309_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCR5848286.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3395 a         3295 c         2947 g         3249 t
ORIGIN      
        1 caacattaac aggcgctgca tgtgacacct gttaatgata aaggaatata ggatgttgta
       61 actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac ggtgttatta
      121 gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca ggtcattagc
      181 aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt ctttttctgc
      241 caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct ggctccagag
      301 ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt ctccgttacg
      361 ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg ccggaagtgt
      421 gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga acttggtcat
      481 accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga aacgcccggt
      541 gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg gaataatttc
      601 ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg cttcacctac
      661 ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac cgttgaggat
      721 tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt tgagcggata
      781 tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa acaggtaaaa
      841 gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca gtttagccac
      901 ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga agctgttagc
      961 ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat agcgcagcac
     1021 cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga tgatccaacc
     1081 tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg tcagttttgc
     1141 cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc tgatacccgc
     1201 ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt tcaggaattc
     1261 atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac gcgcgtcgct
     1321 gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac catcctgcgt
     1381 gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc cagaattcag
     1441 atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca ggaagttacc
     1501 ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc gacgatttag
     1561 tgttttcaca atgggttccc ttctgattag tcatacaacc cgtttgaatt ggtgcgacag
     1621 gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga ttaatgagca
     1681 gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg ccttttcctg
     1741 gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa agatgtcaga
     1801 caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc ttcgagcgat
     1861 acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc gttcggtacg
     1921 atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt tgttgccagg
     1981 tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga ccatcgacag
     2041 gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc accagcccct
     2101 tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta tcgccacatt
     2161 catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc atgaccgtca
     2221 gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg gaccgtgagt
     2281 aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt agcaaattga
     2341 tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca ataccgagac
     2401 gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca atcaccgaat
     2461 ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat gttacctgcg
     2521 gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga ctccccgctc
     2581 cgccaatgac taccgaggca ataaccgaca caataaacgc gatggcgggc atcatctgtt
     2641 ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa ggcacgatca
     2701 acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag aaatccagac
     2761 tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg gcaatcagac
     2821 agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc agacgcttat
     2881 cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa ttgataacca
     2941 gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc acataactga
     3001 tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc gccattacgc
     3061 cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca ccaataaaat
     3121 agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga ccttcctgga
     3181 acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata caattgcggg
     3241 tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt cgctaacagc
     3301 ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat tctcatggct
     3361 gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt tgttgttctt
     3421 cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg cgacagcagg
     3481 tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt tgcttaccgt
     3541 cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca tgttgttcga
     3601 tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga cgaaagcgag
     3661 agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc attaataaac
     3721 cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa gcgagttacc
     3781 tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa caatcgccac
     3841 aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa actcccccgc
     3901 gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct gcattggcgt
     3961 caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga caaacgtccg
     4021 ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt gcagaacggt
     4081 tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag ccgcatttcg
     4141 cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat cagacacacc
     4201 gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc gcccttgcaa
     4261 catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa taccctgttc
     4321 catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc cgacgcgttg
     4381 ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca ctttggaaat
     4441 agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc cgtcaacggt
     4501 acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa tataatccgc
     4561 gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta aaattgcatt
     4621 atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac ctgcgagtat
     4681 atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga tattacagaa
     4741 ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca cgccaccaaa
     4801 ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc attccagacg
     4861 tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg aaaggccgaa
     4921 ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg tgattgcagc
     4981 aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat tcgccagaac
     5041 cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta acaaacgcgc
     5101 accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat caaaacgagc
     5161 cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg ctgccgcagc
     5221 tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa agcgttttgc
     5281 cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt taaccgccag
     5341 ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt ccagtacgca
     5401 ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag caatgcgcga
     5461 gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat atacgccttg
     5521 cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca tcacattcgg
     5581 cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc cacgcatcac
     5641 ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg atgacttatg
     5701 actatatggg gcaaatatgg ttacgccagt aagcatctgc aattacatat ctcttcctga
     5761 tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg attttattga
     5821 tgcactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca ccaactcagc
     5881 cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga cttcttttcg
     5941 gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc ctgtcaagaa
     6001 tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc ctatttattt
     6061 agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc caaagcctgt
     6121 cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt ttatgaatga
     6181 ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag cgcatacacc
     6241 acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac gccagtttga
     6301 tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc gattatggaa
     6361 taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca ccgccattct
     6421 cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg attcattagg
     6481 ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt tagcagaaca
     6541 agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat tgcactatca
     6601 ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag catcattaag
     6661 tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg taaaaaatga
     6721 aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct cccctgtcag
     6781 accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt aaaccgtgat
     6841 gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa cgcgatccag
     6901 atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat cactttttcg
     6961 caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt ggcgtggcta
     7021 cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt tgtccctgtt
     7081 ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt cggcatcatg
     7141 ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt aatgaccagt
     7201 caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc cagccacgta
     7261 ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg tgtggctttt
     7321 gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc tccggccggg
     7381 aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat ggtcttaggt
     7441 ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt cttcgcgatt
     7501 ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt actgcctagt
     7561 gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc attgatgagc
     7621 atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag ctatatcgag
     7681 ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt actgttatta
     7741 ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa tcaatatgcg
     7801 tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact gctgctacct
     7861 gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat cgcgatgatg
     7921 atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc taccgacgtc
     7981 gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc gttggtaggt
     8041 aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc ggtgcctgct
     8101 tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac actcgaagaa
     8161 cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt taatgatttc
     8221 caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc ccatcaggcg
     8281 ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg agctgcgtag
     8341 gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa ccatcagtac
     8401 ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa tcatcgctat
     8461 ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat tggcactggg
     8521 ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg ctttctgttg
     8581 cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa ttcgcagacc
     8641 gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg ccaccatcat
     8701 gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg cactgttcat
     8761 gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta acggcagcaa
     8821 caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt gttatcctgt
     8881 gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt cactctttag
     8941 ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg gcatcggtca
     9001 attcattcat ttgacttata cttgcctggg caatattacc ccctacaact aattacttgc
     9061 caaggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc cattgggtcg
     9121 cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc tgtctccgct
     9181 ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg cgtgtattac
     9241 tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc gtatgctgga
     9301 tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata agcgcggcgt
     9361 actggtaaaa cttaccacca gcggcgcggc aatatgcgaa caatgccatc aattagttgg
     9421 ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg caacacttga
     9481 gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg tccagacgca
     9541 atactgacgc tattaccatt catagcattt tggactggat cgaggacaac ctggaatcgc
     9601 cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac ctgcaacgga
     9661 tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt aagatgacgg
     9721 aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca gaacgatatg
     9781 gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat gttccgccgc
     9841 ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca ttaaatcatt
     9901 acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc actttcatcc
     9961 gcaatagcag ctgcgcttat tctcttttcc gcgcagagcg ttgcggaaca aaccacgcag
    10021 ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga acaaccaccg
    10081 tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg cgtgccctat
    10141 tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt attaacttcc
    10201 cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc cggtcataat
    10261 gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata gtgccgcact
    10321 tgccagtccg actacgggca ccagtaacga taacggtgca acccgccagg tttcatagcg
    10381 tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg ccagatacat
    10441 cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg atgcggaacc
    10501 atcgagaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc tccagattac
    10561 cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga agatgttgcc
    10621 acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg ccacatgctg
    10681 accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg cgatccccgc
    10741 caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga tagtaaaaaa
    10801 cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt taatggcgca
    10861 gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga gcaaattcag
    10921 tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca taaagcgcaa
    10981 accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca caaaatttag
    11041 cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca ttctaccgcc
    11101 tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct ttaacagttg
    11161 attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat acttcggatt
    11221 tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct ggtagagcgt
    11281 cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt attttagaga
    11341 tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt cgccagtaca
    11401 gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt ggcgtcgttt
    11461 ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat atgttactgg
    11521 caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac gtgacgctgg
    11581 ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctacc gtgctgaaag
    11641 cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca atcaactaca
    11701 ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg gtgatgcaga
    11761 gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg cttttcattc
    11821 aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt gtctggtcgc
    11881 cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt tttctggctt
    11941 cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg attgctgatg
    12001 gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc atggtggtta
    12061 cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg ctgatgacag
    12121 caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc aacaacctgc
    12181 tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat gcgccgggcg
    12241 agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat ttttccgccc
    12301 agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta gtgctaacca
    12361 gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct gcgtgggtgc
    12421 tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt tgatttaagt
    12481 cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa tatctttctc
    12541 attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg aggcaaataa
    12601 tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt tctggttttc
    12661 tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc cctcacctaa
    12721 ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg aaaaaaaccg
    12781 ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt tacgctaaca
    12841 ggcattttcc tgcactgata acggatcgtt gacacagtag catcag
//
LOCUS       DAJTQQ010000099        12798 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000099 DAJTQQ010000000
VERSION     DAJTQQ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12798
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.099"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(24..778)
                     /locus_tag="OO309_003251"
                     /pseudo
     CDS             complement(24..778)
                     /locus_tag="OO309_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1154..2794
                     /gene="trg"
                     /locus_tag="OO309_003252"
     CDS             1154..2794
                     /gene="trg"
                     /locus_tag="OO309_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR5848287.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2832..3755)
                     /locus_tag="OO309_003253"
     CDS             complement(2832..3755)
                     /locus_tag="OO309_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5848288.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3972..5315
                     /locus_tag="OO309_003254"
     CDS             3972..5315
                     /locus_tag="OO309_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5848289.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            5540..7195
                     /gene="opgD"
                     /locus_tag="OO309_003255"
                     /gene_synonym="mdoD"
     CDS             5540..7195
                     /gene="opgD"
                     /locus_tag="OO309_003255"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR5848290.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            7335..7559
                     /locus_tag="OO309_003256"
     CDS             7335..7559
                     /locus_tag="OO309_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR5848291.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            7622..8158
                     /gene="rimL"
                     /locus_tag="OO309_003257"
     CDS             7622..8158
                     /gene="rimL"
                     /locus_tag="OO309_003257"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR5848292.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(8153..9133)
                     /locus_tag="OO309_003258"
     CDS             complement(8153..9133)
                     /locus_tag="OO309_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848293.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            9257..10249
                     /gene="tehA"
                     /locus_tag="OO309_003259"
     CDS             9257..10249
                     /gene="tehA"
                     /locus_tag="OO309_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR5848294.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            10246..10839
                     /gene="tehB"
                     /locus_tag="OO309_003260"
     CDS             10246..10839
                     /gene="tehB"
                     /locus_tag="OO309_003260"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR5848295.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            11142..11798
                     /locus_tag="OO309_003261"
     CDS             11142..11798
                     /locus_tag="OO309_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR5848296.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(11816..12322)
                     /gene="tnpA"
                     /locus_tag="OO309_003262"
                     /pseudo
     CDS             complement(11816..12322)
                     /gene="tnpA"
                     /locus_tag="OO309_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            12330..12798
                     /locus_tag="OO309_003263"
                     /pseudo
     CDS             12330..12798
                     /locus_tag="OO309_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         3309 a         3000 c         3176 g         3313 t
ORIGIN      
        1 agtttttcaa tttcctatac gtgaacagct ttatggttaa tctgtttccc ttctcgatga
       61 agagacagcg ttaccctacg gtatccgtat cggcctctat tctcatgata aatctcacca
      121 atacgctttt taacgtccgc atacttgtca ggcttgctga gagcctttag atggtaataa
      181 aacgtactgc gcggtatctc cgcagccctg agaagctcat caagaggata aaactgcctt
      241 agctcgttga gtactttcac tttttcgtgg gatgagctaa ggctttcagc ttttttagat
      301 acacaagccg cgtttcaaga aatcgaactt gcctttcaag atcctcaatg cgtcggtctt
      361 ttgacagctc caatgctgat gccgcttttt ctggatcaac tgatattgca atgtttcttt
      421 tggtgccaat cttgagcgcg cgtaaaccag cttctccgcg ctcttcataa accttcagcc
      481 acctggctac agaaccacta ccagcaagca taaagtgagc agcagcctga ttaagggaca
      541 tgtgctgctc aatcacagct ttcacgacct taatacgcaa ctctggatca gcactaacac
      601 ctttaggttt gggaattaag cctttttctc catgtttttc atagagggca acccatgtcc
      661 tgacctgggt tcgggggaca ccaaaacgtg ccgagatgat cctgtaacca tcatcagtag
      721 tgaagtagtg attcacgact tcaaggcgct tttcaaaagg gtattttggc tttgacatat
      781 taggggctat tccatttcat cgtccaacaa aatgggtgca gtacagttga tagaaatatc
      841 ggtcggggcc ttcgtctttc tgattcccgg ttagcctgaa aacagaaagt ctcaggcacc
      901 cgcaggcatc ctatgaggtt tccttaggga cgaaaataat cacttcacga aattgcgtgc
      961 tgttttccag aatttttcgt cattcgggtt agccagttta gccattcgtt actctcttca
     1021 ttccaatagc attaattttc tctgcaataa ttgttgtaaa aatgtgacgc aaagaggttt
     1081 ttggtcataa gtaattaccg tcaagtgccg atgactttct atcaggagta aacctggacg
     1141 agagacaacg gtaatgaata caactccctc acagcgatta ggttttttgc atcacatcag
     1201 gctggttccg ttatttgcct gcattcttgg cggtatctta gttctattcg cattaagttc
     1261 tgctctggct ggctatttcc tctggcaggc cgatcgcgat cagcgtgatg ttactgcgga
     1321 gattgagatt cggaccgggt tagcgaacag ttcagatttt ttgcgttcag cccggatcaa
     1381 tatgattcag gccggggctg cgagtcgtat tgcggaaatg gaagcaatga agcgaaatat
     1441 tgcgcaagcc gaatcggaga ttaaacagtc gcagcaaggt tatcgtgctt atcagaatcg
     1501 accggtgaaa acacctgctg atgaagcctt cgacactgaa ttaaatcaac gctttcaggc
     1561 ttatatcacg ggtatgcaac ctatgttgaa atatgccaaa aatggcatgt ttgaagcgat
     1621 tatcaatcat gaaagtgagc agatccgacc gctggataat gcttataccg atattttgaa
     1681 caaagccgtt aagatacgta gcaccagagc caaccaactg gcggaactgg cccatcagcg
     1741 cacccgcctg ggtgggatgt tcatgattgg cgcgtttgtg cttgccctgg tcatgacgct
     1801 gataacattt atggtgctac gtcggatcgt cattcgtcca ctgcaacatg ccgcacaacg
     1861 gattgaaaaa atcgccagtg gcgatctgac gatgaatgat gaaccggcgg gtcgtaatga
     1921 aatcggtcgc ttaagtcgtc atttacagca aatgcagcat tcactgggga tgacagtagg
     1981 gactgttcga cagggcgcgg aagagattta tcgtggcacc agcgaaattt cagctggcaa
     2041 tgcggacctg tcatctcgca ccgaagaaca agcggcggct atcgaacaaa ctgccgccag
     2101 catggagcaa ctcactgcga cggtgaaaca gaatgcggat aacgcgcatc atgccagcaa
     2161 actggcgcaa gaggcttcta ttaaagccag cgatggcggg cagacggttt ccggtgtagt
     2221 aaaaacgatg ggcgctatct ccacgagttc gaagaaaatt tctgagatca ccgccgtcat
     2281 caacagtatt gctttccaga cgaatattct ggcactgaat gctgccgttg aagccgcgcg
     2341 agcgggtgag caagggcgtg gatttgccgt tgtcgccagc gaagtacgga cactcgcaag
     2401 ccgcagcgct caggcagcga aagagattga aggcttgatc agtgaatcag tcaggttaat
     2461 tgacctgggg tcggatgagg tggcaacggc agggaaaacc atgagcacta ttgttgatgc
     2521 cgtcgcgagt gtcacacata tcatgcagga aatcgccgcc gcctcggatg aacaaagtag
     2581 aggcataact caggttagcc aggcgatttc tgaaatggat aaggtgacgc aacagaatgc
     2641 ttctctggta gaagaggcct cagcagcggc ggtgtccctt gaagaacagg ctgcacgatt
     2701 aactgaggcg gtgaacgtat tccgtctgaa caaacattct gtgtcggcag aacctcgcgg
     2761 agcgggtgaa ccagttagtt tcgctacggt gtgaaaatgt tcaaggaggg atcgacagat
     2821 cccttcacct ttcagaacgg cattgatttt cgaatagcgt taatcatcaa ctggcaacca
     2881 gaagagaacg tcgcatctac gcgggtcagt attccaatcg gttcgcctgc accatgtccc
     2941 ggaacaggca gggccaccag cgtggcatga cgcaggtcgt cttttacagc gccagaaggg
     3001 acaaaccaca cgtaatcgta ttcaacggta agttgacgag atagcgaagt agacagcgtt
     3061 tcgatacaac ccgaaggaat tttacagccc tggctctgca ctaatgcatc tgaatgctgg
     3121 cgtggcgcag tgccttctgg tgatacaacg accggccatt ccagcacccg gcttagcgtt
     3181 acgttctcct gaagtagcgg gtgattaggg cggacaacca gcttcaacga ttcaagaaac
     3241 agcagttcgt aattaagccc ggtcatcagt tcaggatctg acatccgacc aatgccgata
     3301 tcgatttccc cggtttttaa acccgccaga atcatagggt tactcattgt cgcaacttgc
     3361 aaggtcgtct ctttttgttg ctgatgaaac tgacctataa ccgaaggtaa tatccccagt
     3421 gccgcagtag gtagtgcacc aaccctgacg acatcattat taagaccttc tttacgatga
     3481 agcgactgtc cggcagtgtt gatggcgtca agaactctga ctgcatgcgt taaaaattgt
     3541 tcgccgggta aggtaagttg cgccccctga cgaccacgct caaacaagcg agcgcccgtc
     3601 agctgctcca gttcattcaa tgtattagag agcgcaggtt gactcaaatt aagggtttca
     3661 gctgcgcgcc ccaaagttcc ttgttgtgcg acagctacga atgtatgaag gtggcgcaaa
     3721 cggatgcgct gactaaacag actatttttt tccataagcg atgttaaaaa cgaagcggtg
     3781 tcgctgacaa gtgaagttgt ttgattatga taacttgatt gcaaaatatt attaacaatt
     3841 aaagcaatta tgttacagca aaatggataa tattgatgtt ttcgcggcga gatcacagtt
     3901 tgtaaattct tcccgccaga gtgaatgcgg ttacctacac tccagattac tgaccactgg
     3961 aggcagacac tatggcgaac agcatcacgg cggatgagat tcgggaacag ttttcgcagg
     4021 caatgtcagc catgtaccag caagaagttc cgcaatatgg cacgctgctg gaactggtag
     4081 ctgatgtgaa tctggctgtg ctggaaaaca atcctcaact gcacgaaaaa atggtaaatg
     4141 cagacgagct ggcgcgactg aatgttgaac gtcatggggc gattcgcgtt gggactgcac
     4201 aagagcttgc tactcttcgg cggatgtttg ccattatggg gatgtacccg gtgagctatt
     4261 acgatctctc gcaggcaggg gtgccggtac attcgacagc atttcggccc attgatgatg
     4321 cttctctggc gcgtaatccc ttccgcgttt ttacatcctt actccgcctt gagcttatcg
     4381 agaacgaaat tttgcgccag aaagcggcgg agattctgcg tcagcgcgat atcttcaccc
     4441 cacgttgtcg acaactgtta gaggaatatg agcagcaggg cggttttaac gaaacacagg
     4501 cacaggagtt tgtgcaggaa gccctggaaa cgtttcgctg gcaccagtta gcaacggtag
     4561 atgaagaaac ctatcgcgcc ttgcacaacg aacatcggtt gattgctgat gtggtctgtt
     4621 ttcctggatg ccatatcaac cacctgacgc cacgtacgct ggatattgac cgggtgcagt
     4681 cgatgatgcc tgaatgcgga attgaaccca aaattctcat cgaggggccg ccgcgccgcg
     4741 aggtaccgat tttactacgc cagaccagct ttaaagcact ggaagagacg gtgttgtttg
     4801 cggggcagaa acagggcacg cataccgcgc gctttggtga aattgagcag cgtggcgtgg
     4861 cattaacgcc gaaagggcga caactgtatg atgatcttct gcgtaacgct ggaaccgggc
     4921 aggataatct cactcaccaa atgcatttac aggaaacctt ccgcactttt cctgacagtg
     4981 agtttttaat gcgtcagcaa gggttggcat ggttccggta ccgtctgacg ccttcgggtg
     5041 aggcgcatcg tcaggcgatt catcctggag acgatccaca gcccttaatt gaacgtggtt
     5101 gggtagtggc gcaacccatc acctatgaag atttcttgcc cgttagcgcg gcggggatct
     5161 tccagtcaaa tctgggtaat gaaacgcaga cacgcagtca cggtaatgcc agtcgcgaag
     5221 catttgagca ggcgttgggt tgtccggttt tggatgagtt ccagctttac caggaagcgg
     5281 aagaacgcag taaacgtcgc tgtggtttgc tttaaattct gaccatccgc ctttgcaaaa
     5341 atttgcctga ttttacaaac gaatcaggct catcccatcg acataaaaaa aatgccgatt
     5401 tatgcatatt ctctcagttc aacaattgga ttattaataa atattgtcta gagtgagcgg
     5461 tcataaataa gcactttctt gccgctgaaa acgaccagcg cgggaccatt cacaacacca
     5521 gaaggactca ctttcaggta tggatcgtag acgatttatt aaaggttcaa tggctatggc
     5581 cgccgtgtgc ggtaccagcg gcattgcttc tcttttttct caggcagcat tcgcggcaga
     5641 ttctgatatt gccgacgggc aaacccagcg ttttgacttc tccattctac agtcaatggc
     5701 gcacgactta gcgcaaacag cgtggcgtgg tgcgcctcgt ccgttacctg acacgctggc
     5761 gacaatgacg ccgcaggctt ataacagtat tcaatacgac gccgaaaaat cgctctggca
     5821 taacgttgag aaccgtcaac tggacgctca gttcttccat atgggaatgg gattccgtcg
     5881 ccgcgttcgt atgttttctg tagatccagc aacacatctg gcgcgtgaaa ttcactttcg
     5941 cccggagttg ttcaaataca acgatgcagg tgttgatacc aaacaattag aagggcaaag
     6001 cgatctcggc tttgccggtt ttcgcgtgtt taaagccccc gaactggcgc gccgtgatgt
     6061 agtatcattt ctcggcgcga gttatttccg cgccgttgat gatacatatc aatacggttt
     6121 gtcggcccgc ggcctggcga tcgacactta caccgacagt aaagaagagt tccccgactt
     6181 taccgccttc tggtttgata cggtaaaacc gggggcaact acctttaccg tttatgcgtt
     6241 gctcgatagc gccagcatta ctggtgccta taagttcact atccattgtg agaaaagtca
     6301 ggtgattatg gatgtggaaa atcacctgta tgcgcgcaaa gacattaaac agctgggcat
     6361 ttcgccgatg accagtatgt tcagctgcgg tactaatgaa cgtcggatgt gcgatacaat
     6421 tcatccgcaa attcatgact ctgatcgtct gtccatgtgg cggggcaacg gcgagtggat
     6481 ttgccgtccg ctgaataatc cgcaaaaatt gcagttcaat gcttacaccg acaacaaccc
     6541 gaaagggttt ggtttattgc aactggatcg tgacttctcc cattatcagg acattatggg
     6601 ctggtataac aaacgcccaa gtctgtgggt ggaaccgcgt aacaagtggg gtaagggcac
     6661 catcggcctg atggaaatcc caacaacggg cgaaacgctg gataacattg tctgcttctg
     6721 gcagccagaa aaagctgtaa aagcaggtga tgagtttgca ttccagtatc gtctgtactg
     6781 gagtgcgcaa ccgcctgttc attgcccatt agcgcgcgtt atggcgacgc gtaccggcat
     6841 gggcggtttc ccggaaggtt gggcgccagg tgaacactat cccgaaaaat gggcgcgtcg
     6901 ttttgccgtc gatttcgttg gtggtgatct gaaagctgcc gcgccaaaag gcattgagcc
     6961 ggtgattacg ctttccagtg gggaagcgaa gcaaatcgaa attctctata ttgaacccat
     7021 cgatggttat cgtattcagt ttgactggta tccgacttcg gactccactg atccggtcga
     7081 tatgcggatg tatctacgtt gtcaggggga cgctatcagt gaaacatggc tgtatcagta
     7141 tttcccgcca gcgccggata aacgtcagta tgttgacgac cgcgtgatga gttaatcgtt
     7201 ttttcttcgg caccttcttc gggaggtgcc gtctggttaa acacgatccc gctcgcattt
     7261 ttccctaagt taaatgagta atctgatggt gtgtatttca gatacaccgt gtcagccact
     7321 aacagggagt gcgtatgttt ccagaatacc gagatttaat atcccgtctg aaaaacgaaa
     7381 atcctcgctt tatgtccttg ttcgataaac acaataaact tgatcatgaa attgccagaa
     7441 aggaaggttc cgacggtcga gggtacaatg cggaagtggt ccgcatgaaa aaacaaaagc
     7501 tacagttaaa agatgagatg ctcaaaatcc tgcagcagga gagcgtcaaa gaggtgtaaa
     7561 ctttcctaag ccgccagcca ggcggctttt ttaacaacag catggattga ctggagataa
     7621 gatgactgaa acgataaaag taagcgaatc acttgaatta catgctgttg cggaaagtca
     7681 cgtcacacct ctttatcagt taatctgtaa aaataaaacc tggttacagc agtcgctaaa
     7741 ctggccgcag ttcgttcaga gtgaagagga cacgcgaaaa acggtgcagg gtaatgtgat
     7801 gttgcatcaa cgcggctatg ccaaaatgtt catgattttc aaagaagatg aacttatcgg
     7861 cgttatctcg tttaatcgta ttgaaccact gaataaaact gctgaaatcg gctactggct
     7921 ggacgaatct catcaggggc aggggatcat ttctcaggcg ctgcagacat tgattcatca
     7981 ttacgctcag tctggtgaac ttagacgctt cgtgatcaaa tgtcgggtgg acaatccgca
     8041 aagcaaccag gtcgctttgc gcaatggttt tatccttgaa ggttgcctga aacaggctga
     8101 gttcctgaat gatgcctatg atgatgtgaa cttatacgcg cgtattatcg attcataata
     8161 accctgccag cggcgttcgc gtaatgcgct cctcgccgtt aatgactttt ggaccacgga
     8221 catgaaccgt gtcgccatca aaggcttcaa cgacggcgtg gtcggttaac tccacatggt
     8281 tttcaattat caccgcacct gtaatacggc tctcaccttg tattacaacg tgctcgtcga
     8341 gcagaatcgg tccaccacgt accacggcat ttccgccaat caagacatga tgttttaaca
     8401 cacaattacc ttccacaatg gcatattccg ccacctgcga actgtaatga atcgtcggaa
     8461 tggcatcttc ttctatgcca gctttcacct gcgcgtgacc gtagacttta gcgcaatcgc
     8521 atagccagac gttgttctct tcattacctt caacactggc aaaatcaaaa acttcggcgc
     8581 gatgttcaat aaaagcatac cggacgacgg catcgccata aatttgtgcc tggtggacaa
     8641 tacgcgaggc gctaaccctt gcgcgatcat aaatttggag caggagttga tggtcgggcg
     8701 ttaagccttg tgcggcgacg atcatagaat gttgatcaat taaggcgtga ccaaatattc
     8761 tacattgcac gtaaactaat gagtcatgaa tggtgacact gtcacttata tatgcaccct
     8821 ggctgatttc actattatcg atccagacgt tatccgttgc gtaaacctct ccccacagca
     8881 cgctggtgcc ggtgatacgc gtattaccgg aaatcaccgt tccgccgaac gcaatagcat
     8941 tctggtcgta aatccagcaa tttccttcct gtgctaacac cgtttcgcga tcgatccagc
     9001 cgccagcggt tcctgctatc acatcgttaa aatcgctaat ggcgataatc tgccgtaata
     9061 acacactttt tttagtgcca tcttcctgat aactaaaggc tcgctgttct tcacttaggc
     9121 gatatttgcg catagcgttt tcccacaggt gacttactat aaccgtagca aattctgcgg
     9181 ctctggctat gctcgagaaa attccataaa atgcatttca aatatacttt ataaattaaa
     9241 caaaatgagt aagaagatgc agagcgataa agtgctcaat ttgccggcag gctactttgg
     9301 tattgtgttg gggacgatag ggatgggatt tgcctggcgc tatgccagcc aggtttggca
     9361 ggtcagccac tggttagggg atgggctggt gattctggcg atgatcatct ggggattatt
     9421 gactagcgca tttattaccc gactcatacg ctttccgcat agcgtgctgg cggaagttcg
     9481 ccatccagtg ctgagcagtt ttgtgagttt gtttccggca acgacgatgc tggtggcgat
     9541 tggttttgtt ccgtggtttc gcccactggc ggtgtgcatg ttcagttttg gtgtcgtggt
     9601 tcagttggct tatgccgcct ggcaaactgc gggattatgg cgcggatctc accctgaaga
     9661 agctaccacg cctggactgt atctgccgac agttgccaac aactttatca gcgcaatggc
     9721 ctgtggtgcg ttgggctaca ccgacgccgg tctggtgttt ttaggcgcag gcgttttctc
     9781 atggctaagc cttgaaccgg tgatcttgca gcgtctgcgc agttcgggag aattacccac
     9841 ggcactgcgg acatcactcg gcattcagct cgctcctgcg ctggtggcct gtagtgcctg
     9901 gctgagcgtc aacggcggcg agggtgacac gctggcgaaa atgcttttcg gttatggact
     9961 gctgcaactg ctgtttatgc tacgtctgat gccatggtat ctctcccagc catttaatgc
    10021 ttcattctgg agtttctcgt tcggcgtatc tgcactggca accaccggtt tgcatctggg
    10081 gagtggcagc gataatggat ttttccatac gctggcggtg ccgctgttta tctttaccaa
    10141 ttttattatt gcaatactgc tcatccgtac ttttgcgctt ctgatgcagg gaaaattgtt
    10201 agtcagaacc gagcgcgccg ttttaatgaa agcagaggac aaagaatgat cattcgtgac
    10261 gaaaactatt ttactgataa atatgaatta acccgcacac actctgaagt actggaagcg
    10321 gtgaaagtgg ttaaaccggg taaaacgctg gatctgggct gtggcaatgg tcgtaacagt
    10381 ctttacctgg cagccaatgg ttatgatgtt gacgcatggg ataaaaatgc catgagtatc
    10441 gccaacgtcg agcgcattaa atccattgaa aatctggata atttacacac ccgggtcgtt
    10501 gatctgaata acctcacatt tgatggacag tacgatttta ttctttcgac tgtggtgctg
    10561 atgttccttg aggctaaaac catcccaggg ttgattgcca atatgcaacg ttgcactaaa
    10621 cctggtggtt acaacctgat tgtggcggcg atggataccg ctgattatcc atgtaccgtc
    10681 ggcttcacgt ttgccttcaa agagggagaa ttacgtcgat attacgaagg ctgggagagg
    10741 gtgaaataca atgaagacgt cggcgagctg caccgcaccg acgccaacgg taatcgtatt
    10801 aaactgcgtt tcgccacgat gctggcacgt aaaaaatgac ccggtaagca caaaacgcgt
    10861 gaaaattccc cacgctgaga tgatttactg ttcttctttt cggtaagcat attttttatc
    10921 gaagggatgt gaaattaatc acagtagtcg aagtttttta gcagcttaac ttactgaaat
    10981 ttaactactg atgattgact tagccccttt ttcggcattg actatgtcgt ctgaaaaggg
    11041 gctgaaaaat ttattttcac caacactttt tttgccacaa cacgaagcgg cgctttttgc
    11101 tataacttag aaagtaatat aatcatctca ggaaactatt catgcgtacc acatcatttg
    11161 cgaaagttgc agctttatgc ggcttgttgg ctctgtctgg ttgtgcctct aaaatcaccc
    11221 agccagataa atataacaat tactctgatt taaaagaaac aacctcagca acaggtaaac
    11281 ctgttttacg ttgggtagac ccgagttttg atcaaagcaa atatgacagc atcgtctgga
    11341 acccaatcac ttattatccg gtaccaaaac cttctaccca ggtagggcag aaagttctgg
    11401 ataaaatttt gaactatacc aacaccgaaa tgaaagaagc gatagcgcag cgtaaaccag
    11461 tggttaccac cgctgggccg cgtagtctga ttttccgtgg ggccattacc ggtgtagata
    11521 ccagcaaaga agggctgcaa ttctatgaag tggttcctgt tgcattagtg gttgcgggga
    11581 cgcaaatggc tacaggccac cgtaccatgg atactcgcct ctattttgaa ggtgagctga
    11641 ttgatgcagc gactaataaa ccggttatca aagtcgttcg tcagggcgaa ggtaaagacc
    11701 tgaataacga aagtacgcca atggctttcg aaaatattaa acaagttatt gatgacatgg
    11761 cgaccgatgc caccatgttt gacgttaaca aaaagtagtt ccagacgcgc catcgttaga
    11821 tggcgctttt tatccggtgc gccgtaaaac cccatccttc agggcgggga tataaggcgc
    11881 ggttttccac ctgaccaggt gtttgctgtt gttcaatgta ttggcggatg acggatattg
    11941 gcgcacctcc gcagctactg gcaaaatagc caggactcca caaaacgcct ttgtagtaat
    12001 acctgactgc aatatctggt cgatctcgtc gcagtaacct acccgatacg cctttgaggc
    12061 tgtttaccag actggatatc gccagtttgg gaggttagtt aattaacaaa tggacgtgat
    12121 ctggttcgcc atccatttca accagttcag cttcaaaata agcacataca tttgaaaagt
    12181 aagtgcgtag tttttctgtc gcgtcatggt caaaaatctg gcgtcggtat ctggtgacaa
    12241 agactaggtg aacatgcatc aggaaaacac aatgccttcc acgccggata tcggtttctt
    12301 ttttcacaga ccaaagtatc atttgacctg tgaaacgatt acaggcattt aaattccagt
    12361 taagaccagg tggtcaacag gagtgtgaaa tgaggcgctt cgcaggcgca tgtcgtttcg
    12421 ttttcaatcg tgctctggca cgtcagaatg agaatcataa ggccggtaat aaatacatcc
    12481 cttacgggaa aatggcttcc tggctggttg agtggaaaaa tgccactgaa acgcaatggc
    12541 ttaaagattc tccctcacag ccattgcaac agtcactgaa agaccttgag cgggcttaca
    12601 aaaacttctt ccggaagcgg gctgcttttc cccgattcaa aaagcggtga cagaatgatg
    12661 cattccgcta cccgcagggc gttaagctcg atcaggaaaa cagccgtatt tttctgccga
    12721 aactgggctg gatgcgctac cggaatagcc gtcaggtcac gggtgttgtg aaaaatgtca
    12781 ctgtcagcca gtcctgcg
//
LOCUS       DAJTQQ010000100        12701 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000100 DAJTQQ010000000
VERSION     DAJTQQ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12701
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.100"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(105..743)
                     /gene="rutR"
                     /locus_tag="OO309_003264"
     CDS             complement(105..743)
                     /gene="rutR"
                     /locus_tag="OO309_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR5848297.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1031..2122
                     /gene="rutA"
                     /locus_tag="OO309_003265"
     CDS             1031..2122
                     /gene="rutA"
                     /locus_tag="OO309_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR5848298.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMEFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2122..2814
                     /gene="rutB"
                     /locus_tag="OO309_003266"
     CDS             2122..2814
                     /gene="rutB"
                     /locus_tag="OO309_003266"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR5848299.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2826..3212
                     /gene="rutC"
                     /locus_tag="OO309_003267"
     CDS             2826..3212
                     /gene="rutC"
                     /locus_tag="OO309_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR5848300.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3220..4020
                     /gene="rutD"
                     /locus_tag="OO309_003268"
     CDS             3220..4020
                     /gene="rutD"
                     /locus_tag="OO309_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR5848301.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4030..4620
                     /locus_tag="OO309_003269"
     CDS             4030..4620
                     /locus_tag="OO309_003269"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR5848302.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4631..5125
                     /gene="rutF"
                     /locus_tag="OO309_003270"
     CDS             4631..5125
                     /gene="rutF"
                     /locus_tag="OO309_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR5848303.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5146..6474
                     /gene="rutG"
                     /locus_tag="OO309_003271"
     CDS             5146..6474
                     /gene="rutG"
                     /locus_tag="OO309_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR5848304.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6556..6663)
                     /locus_tag="OO309_003272"
     CDS             complement(6556..6663)
                     /locus_tag="OO309_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848305.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7036..7632
                     /gene="wrbA"
                     /locus_tag="OO309_003273"
     CDS             7036..7632
                     /gene="wrbA"
                     /locus_tag="OO309_003273"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5848306.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7653..7880
                     /locus_tag="OO309_003274"
     CDS             7653..7880
                     /locus_tag="OO309_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR5848307.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7918..9159)
                     /gene="agp"
                     /locus_tag="OO309_003275"
     CDS             complement(7918..9159)
                     /gene="agp"
                     /locus_tag="OO309_003275"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR5848308.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9452..10708)
                     /locus_tag="OO309_003276"
     CDS             complement(9452..10708)
                     /locus_tag="OO309_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCR5848309.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     NENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     EPFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10969..11889
                     /gene="cbpA"
                     /locus_tag="OO309_003277"
     CDS             10969..11889
                     /gene="cbpA"
                     /locus_tag="OO309_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR5848310.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11889..12194
                     /gene="cbpM"
                     /locus_tag="OO309_003278"
     CDS             11889..12194
                     /gene="cbpM"
                     /locus_tag="OO309_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR5848311.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12287..>12701)
                     /gene="torD"
                     /locus_tag="OO309_003279"
     CDS             complement(12287..>12701)
                     /gene="torD"
                     /locus_tag="OO309_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5848312.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         3033 a         3240 c         3202 g         3226 t
ORIGIN      
        1 aacgttcgct gagtgtgaat accgctgccg ctggcggtaa cgccagttta atgacgatcg
       61 gttaaacagg tagccggagg atgttacaac ctcctccgtc atctttaacg tggtcgaatc
      121 ccctcaataa taatccgctg cacgttttca accgtttgat tgaaaaatac ctcatcgcgc
      181 aacgtcgcgc ctgtcaccgc ctccacctga ggggcgaaat cggcgtaatg ttgagtggaa
      241 gcccaaatca taaaaatcaa atgctgcgga tcaatcggcg cgagtttgcc gcttttgacc
      301 caaccggcaa tcagcgccga tttctcatca attaatgcct tcaaatcgcc cgtcagttca
      361 tccattaaca acggcgcgcc tgccagcatc tccatacaga acaggcgcga agcctgcgga
      421 taatcgcgtg agacttccag cttcagacgg atgtactctt ttatcgccgc cagcggggcg
      481 aaatcttcac gaaacgcttt taacggtgcc agccagatat cgagaatctg ccgcagcacg
      541 gcaatataca gcgcctcttt tgacgggaag taatacagca gattggtttt tgaaacaccc
      601 gccaactctg cgatctgctc cagccttgtg ccgtgaaaac cgaattgtga aaaagtgtcc
      661 agtgctgcgc taagaatcgc ttttttcttc gcgcttactg cgcgcgaacg tttacccgtt
      721 gttttcactg cgccttgcgt catgcgctct cccctcttgt agattggtaa caggatagca
      781 aaacccccgc ccggcctcga ccttctgcac tctcatcgcg cacggatgca tgttttatgt
      841 gcaactgttt tgaccgttta gtccactttt taccagatat tttagataac acaatgctaa
      901 cacattaata atattcactt tttaaaaact ggcatccgct ttgcgaacaa gccaatacgc
      961 ggctgaaaag gatatgcagg atgcagcgcc ccgtctgact ttcactttac gagacgaaga
     1021 gaggttgatg atgaaaattg gcgtattcgt acctattggc aacaacggct ggctcatttc
     1081 gacccacgcg ccgcagtaca tgccgacctt tgaactgaat aaagccattg cgcaaaaagc
     1141 ggagcattac catttcgatt tcgccctgtc gatgatcaaa ctgcgtggct ttggcggcaa
     1201 aactgagttc tgggatcaca accttgagtc gttcaccttg atggcgggtc tggcagccgt
     1261 gacctcgcgc attcagattt acgccactgc tgccacctta acgttacctc cggcaatcgt
     1321 cgcccgtatg gccgcaacca tcgactccat ctctggcggg cgttttggcg tcaacctcgt
     1381 gactggctgg caaaagcccg agtatgagca gatgggtatc tggcctggcg atgactattt
     1441 ctcccgtcgt tacgactatc tcaccgaata tgttcaggtg ctgcgcgacc tgtggggcac
     1501 ggggaaaagc gattttaaag gcgatttttt caccatgaat gattgtcgcg tcagtccgca
     1561 accgagtatc cccatgaaag tgatctgcgc cgggcaaagc gacgctggca tggagttctc
     1621 cgcccagtat gccgatttca acttctgttt cggcaaaggc gtaaatacac ccacggcttt
     1681 cgccccgacc gctgcgcgga tgaaacaggc cacagagcaa accggacgcg acgttggctc
     1741 ttatgtgttg tttatggtga ttgccgatga aaccgacgat gccactcgcg ccaaatggga
     1801 acactacaaa gcgggcgcgg atgaagaggc gttaagctgg ctaaccgaac aaagtcagaa
     1861 agatacccgc tccggtactg acaccaacgt tcgtcagatg gccgatccca cttcggcggt
     1921 aaacatcaat atggggacgt tagtcggttc ttacgccagt gtcgcgcgca tgttagatga
     1981 agtcgcaagc gtgcctggtg cagaaggcgt gctgttgacc ttcgatgatt ttctgtcggg
     2041 aatcgaaacc ttcggcgagc gcattcaacc actgatgcag tgccgcgccc atctctctgc
     2101 gctgactcag gaggtggcat gatgacaacc ttaaccgctc gaccggaagc cattaccttc
     2161 gatccgcagc aaagtgcgca gatcgtggtg gatatgcaaa acgcttatgc cacgccaggc
     2221 ggctacttag atctcgccgg gtttgatgtc tcaaccactc gcccggtcat tgccaacatt
     2281 caaaccgctg tgaccgcagc gcgagcggca gggatgttga tcatctggtt tcaaaatggc
     2341 tgggatgaac agtatgtcga ggctggcggc cccggctcac cgaattttca taaatcgaac
     2401 gccctgaaaa ccatgcgtaa gcagccgcag ctgcagggga aattgctggc gaaaggctcc
     2461 tgggattatc aactggtgga tgaactggtg ccgcagcctg gcgatattgt gctgccgaag
     2521 ccgcgctaca gcggtttctt caatacgccg ctggacagca ttttacgcag ccgtggaata
     2581 cgccatctgg ttttcaccgg tatcgctacc aacgtctgcg tcgaatcgac gctacgcgat
     2641 ggcttttttc tggagcattt cggcgtggtg ctggaagacg caacacacca ggcggggccg
     2701 gaatttgcac agaaagccgc gttgttcaat atcgaaacct tttttggctg ggtcagcgac
     2761 gtcgaaacgt tctgcgacgc gctttctccc acgtcctttg ctcgtatcgc ttaaggagtt
     2821 tagcgatgcc aaaatccgta attattcccg ctggcagcag cgcaccgctg gcccccttcg
     2881 ttcccggcac gctggctgat ggcgtggtgt atgtctccgg tacgctggct tttgatcaac
     2941 ataataacgt gctgtttgcc gatgacccaa aggcgcaaac ccgccacgtt ctggaaacta
     3001 tccgcaaggt gatcgagacg gcgggtggca cgatggcgga tgtgaccttc aacagcatct
     3061 ttattaccga ctggaaaaat tacgccgcga ttaacgaaat ctacgccgag ttttttccgg
     3121 gtgataaacc ggcgcgattc tacattcagt gcggactggt aaaacctgac gcgctggtgg
     3181 aaatcgccac aattgcgcat atcgccaagt gaggccgcga tgaaactttc actctcatct
     3241 cccccttatg ctgatgcacc cgtagtggtg ttgatttcgg gtcttggcgg tagcggcagt
     3301 tactggttac cgcaactggc ggtgctggag caggagtatc aggtagtctg ttacgaccag
     3361 cgcggcaccg gcaataatcc cgacacactg gcagaagatt acagtatcgc ccagatggca
     3421 gcggaactgc atcaggcgct ggtagccgca gggattgagc gttacgcggt ggtcggccat
     3481 gcgctcggtg cgctggtggg gatgcagctg gcgctggatt atcccgcgtc ggtaactatg
     3541 ctggtcagcg ttaacggctg gctacgaata aacgcccata cgcgccgctg ttttcaggtt
     3601 cgcgaacgat tactgtatag cggcggcgcg caagcatggg tggaagcgca gccgttgttc
     3661 ctctatcccg ccgactggat ggcggcccgc gcacctcgcc tggaggcaga agacgcgctg
     3721 gcactggcgc attttcaggg caaaaataat ttactacgtc gacttaacgc cctcaaacgc
     3781 gctgacttta gtcaccatgc ggatcgcatc cgctgcccgg tgcaaatcat ctgcgccagt
     3841 gatgatctgc tggtgccaac agcatgttcc agtgaacttc atgccgccct gcccgatagc
     3901 cagaaaatgg tgatgcccta tggcggacac gcctgcaacg tgaccgatcc cgaaacgttt
     3961 aatgctctgt tactcaacgg gcttgccagc ctgttacatc accgtgaagc cgccctgtaa
     4021 ggaattgcta tgaacgaagc cgttagccca ggtgcgctta gcaccctgtt caccgatgcc
     4081 cgcactcaca acggctggcg ggagacaccc gtcagcgatg agacgttaca ggagctttat
     4141 gccctgatga aatgggggcc gacatcagct aactgttctc cggcacggat cgtgtttatc
     4201 cgcactgcag aaggaaaaga acgtctgcgc ccggcgcttt ccagcggcaa tctgcaaaaa
     4261 accctgaccg cgcccgtcac cgctatcgtc gcctgggaca gtgaatttta tgaacggtta
     4321 ccactactgt ttccccacgg tgatgcccgc agttggttta cctccagccc acaacttgcc
     4381 gaagaaacag cgtttcgcaa cagttccatg caggcggctt atctgatcgt cgcttgccgt
     4441 gcgctgggac tggataccgg cccgatgtcg ggttttgacc gtcaacacgt tgacgacgcc
     4501 ttttttgcgg gcagcacgct gaagagcaat ctgctgatta atatcggcta tggcgatagc
     4561 agcaaacttt ttgcgcgcct gccacgtctg tcctttgaag aagcctgcgg gctgttgtaa
     4621 ggagcaaact atgaacatag ttgatcaaca aacctttcgc gatgcgatgt cctgtatggg
     4681 cgcggcggtc aatatcatca ccacggacgg tccagccggg cgcgccgggt tcaccgccag
     4741 cgccgtctgc agcgtgaccg acacgccgcc cacattactg gtgtgcctga atcgtggggc
     4801 gtccgtctgg ccggtattca atgaaaatcg aacgctgtgt gtaaatacac tgagcgccgg
     4861 gcaggagccg ctttcaaatc tttttggcgg caaaacgccc atggaacacc gctttgccgc
     4921 cgcccgctgg cagacgggtg tgaccggatg cccgcaactg gaagaggctc tggtttcgtt
     4981 tgactgccgt atcagccagg tggtgagcgt cggcacccac gacattctgt tttgcgccat
     5041 cgaagcgatt catcgtcaca ccacacccta cgggctggtg tggttcgatc gcagttatca
     5101 cgcgctgatg cgccctgctt gttaacctcc ttaaggagac agctgatggc aatgttcggt
     5161 tttcctcact ggcagttaaa atcgacctct acagagagcg gcgtggtcgc gccggatgaa
     5221 cgattgccgt ttgctcagac ggcagtaatg ggcgttcaac acgcggtggc gatgtttggc
     5281 gcgacggtgt taatgccgat tctgatgggg ctggatccca atctttccat tttaatgtcg
     5341 gggatcggca cgttactgtt tttctttatt accggcgggc gcgtccccag ttatctcggc
     5401 tccagcgcgg cttttgtcgg cgtggtgatt gccgcgacgg gctttaacgg tcagggcatt
     5461 aacccgaata tcagcatcgc gctgggcggg attatcgcct gtgggctggt ttataccgtc
     5521 attggcctgg tggtgatgaa aattggtaca cgctggattg aacgactaat gccgccggtg
     5581 gtgacgggtg cggtggtgat ggcgattggc ctgaacttag cgcctatcgc agtgaaaagt
     5641 gtatcggctt cggcctttga tagctggatg gcggtaatga cggtgctgtg tattgggctg
     5701 gtggcggtgt ttacccgtgg catgatccag cggttattga ttctcgtagg attaattgtc
     5761 gcctgtctgc tgtatggcgt gatgaccaac gttttagggc tgggaaaagc cgtcgatttt
     5821 actctcgtca gctatgctgc ctggttcggt ctgccgcatt tttccactcc tggttttaat
     5881 ggccaggcga tgatgttgat tgcgcccgtg gcggtgattc tggtggcgga aaacttaggt
     5941 catctcaagg cggtcgccgg gatgaccgga cgcaatatgg acccgtatat ggggcgagca
     6001 ttcgtcggtg atggtctggc aacgatgctt tccggctctg tcggcggtag cggcgtcacc
     6061 acctatgctg aaaatatcgg tgtgatggcc gtgacaaaag tctactcgac gctggtgttt
     6121 gttgcagctg ccgtcatcgc tatgctgttg ggcttttcgc cgaaatttgg cgcgctgatc
     6181 cataccattc cggccgcggt gattggtggt gcatcaattg ttgtgttcgg gctgattgcc
     6241 gtcgcaggcg caaggatatg ggtacaaaac cgtgtcgatt taagccagaa cgctaatttg
     6301 attatggtcg cagtaacctt cgtgctgggc gcgggtgatt ttgctctcac gcttggcggt
     6361 tttacgttgg gagggatcgg tacagcaacc tttggcgcga ttttactcaa tgcgttatta
     6421 agcagaaggt tggttgacgt tccaccgcca gaagttgttc atcaggagcc ataaacagaa
     6481 aaccgccggg ttaccggcgg tgatgacttt ttgacgagct taagaaaaaa atgcattcgt
     6541 cattgattag cccggctagt tgtcgctttt gccgtgactg ctcttgccac cttttttacc
     6601 tgcttctgat gctctttcgc ggtcttctgc aaaattgccg gaaccgcctc gatggtttgc
     6661 cattactgac ctccgtctgg gtatagacat catattgcat agaattaacg cggcgtatgg
     6721 cggctgccat attttcgcta cgtgggacta agcttagtga actgacgcgg acagacctgc
     6781 gtttggtaat atcctgcaac agcttcttag cgagaataga tatcatctcc aataattatc
     6841 cataagccgc tcttatgatt aaaaaaacgc gatgagataa aaatgtgtca ttttgcgaca
     6901 aaattacgtg cttgtgaaag ttgttataaa tcaaataagt ggttgtgaaa tttgcactct
     6961 gaaaaggacg tcttatcttt aaataagtgg tagcgaatcg ctacggaata gagataacac
     7021 gaggagtggt tagaaatggc taaagttctg gtgctttatt attccatgta cggacatatt
     7081 gaaacgatgg cacgcgcagt cgctgagggt gcaagcaaag tggatggcgc agaagttgtc
     7141 gttaagcgtg taccggaaac catgccgccg caattatttg aaaaagcagg cggtaaaacg
     7201 caaactgcac cggttgcaac cccgcaagaa ctggccgatt acgacgccat tatttttggt
     7261 acacctaccc gctttggcaa catgtccggt caaatgcgta ccttcctcga ccagacgggc
     7321 ggcctgtggg cttccggcgc actatacgga aaactggcga gcgtctttag ttccaccggt
     7381 actggcggcg gtcaggaaca aaccatcaca tccacctgga cgacccttgc gcatcacggc
     7441 atggtgattg tccccattgg ctacgcagcg caggaattat ttgacgtttc acaggttcgc
     7501 ggcggtacgc cgtacggcgc aaccaccatc gcaggcggtg acggctcacg ccagccaagc
     7561 caggaagaac tgtctattgc tcgttatcaa ggggaatatg tcgcaggtct ggcagttaaa
     7621 cttaacggct aatcttcaac aggaggatac gcatgccaac tcaagaagcg aaagctcatc
     7681 acgtcggtga atgggcatct ctgcgcaata cgtcgccgga aatagccgag gccattttcg
     7741 aagtcgccgg gtacgacgaa aaaatggcag aaaagatttg ggaagaaggt agtgatgaag
     7801 tgttagtcaa agcctttgct aagaccgata aagattcgct tttttggggc gaacagacca
     7861 tcgaacgtaa aaacgtttaa ccagcgactc ccccgcttct cgcgggggag ttttctgtta
     7921 tttcaccgcc tcattcaaca cgctatcaaa cttatccatc gggcagaaac cgttggcatc
     7981 tatcgggcaa ccgcttaatt ccagcgtcac gcgctgcgca ggtgcctgca gggttaacgc
     8041 atcggcatta cgtaactgtt ccgcactctg gtacacatat tcaattttca tcaaatcgcg
     8101 attggctttg ctgtcatgcc aacgctggaa aacgattttg ccgccaatcg gcgtgcgttc
     8161 gttctggtca tgcaactgat acggtttgaa atccagcgcc gttaacagag aggctatgtt
     8221 ggagtcgtgc ccaactaaca ccgtgatttt cggagcactg gcgcgatcgg tgaccagggc
     8281 tttgtcgata taactgacca gcggtttcgc aacattgcgc gccacttccg gtgaggtaaa
     8341 cagactgtcc tggtaaccgt ttttcagctt cgacaatacc ttccactgct ggtcagattt
     8401 gatttctccc caggccacct gatccatcgg aaaaccttcg tagtattgca aagtaaacgc
     8461 atctaccagc gagttgccga ctttcagcgg cccggaaaca cctggttctt gttgatactt
     8521 ggcgctaaag gtatttttgc catccaccag cgaacactgt tgtttctctt tacaggcagg
     8581 ggaatcttta tagttaacga ttttttccag tagctggtag ctgtcggtaa gctggagttt
     8641 gctgagctct ttctccattg ccgccaccgc ctgttcactg aatgcggcgg aatcatcggt
     8701 gatcatcggg ttaaaggttg ggtccatagt gcccattttt tcctggtgat gcacaggaat
     8761 atcacaccca gggaatgcac cagtaataaa gaactgggcg gtagcgacgg tacgttgcag
     8821 actattggca taggcataaa cggtgtccgg cggcgggcat tcccccgatt tcactatccc
     8881 ctgctctgcc agccattcac gcatgtaatg gcccatatac acttcgagca cgccaccttt
     8941 ggtggtgagt tgcccaccgg ggacgtccca ttcgggccat ttattcggcg tcgactgctc
     9001 cagcacactg ccattgttcg ccagcggcgc acgtaagtta tggcggctca tcatgagcac
     9061 ttgctgtagc tgatagcctt ccggtacggt ttgtgcctga gcatttgaag cgagtaaaac
     9121 tatccctgcc acagttgcgg cgattagcgt tttgttcatt cctggcacct cttttgttat
     9181 caatcattaa agagtgtgac agaaatatga cttttttccg ggaacaggtt cgcaaaatgg
     9241 cttgttgaac gcctgttttt ctatatacat cttcaaaaac aggcaaggtt tgacgcaaac
     9301 tatgcctgat tagcccctgc ctcgcgtacg ccgctgataa ttgcacctgt ggctaaacag
     9361 aatatctgcg gcgttaccaa tacagagaca atatttacca ataaaaaagt gtgagactaa
     9421 taacaatggt ttttagcata tgcctttgcc attaagttgc ataacgattt aaatagtcaa
     9481 gttgcgcatt catttcctta ttttttaact tataagcctg agaaaaaggt tcaggaggga
     9541 ggtaaacatc ataatgattg ccagtgtttg ttaaatatcc atactcaaga tcttcatctg
     9601 ttgaaaagga ttcatttccc aaggagattg atgccacatt actattaaca ataaataatg
     9661 agcaaatgct atctgaaaga acagcaagaa aattcttcac cgctttaatc ctatccgacg
     9721 tgtcggtatc taaagcaagc aaattcttgt tatcattaaa tataaactcg ggtaaatgca
     9781 gtatgcattt tttgtcattc aagcaactat caatgaattg ttttttaaaa aacagaaagc
     9841 tccatgacat aaaactaccc acaccctgaa ttttgttgtc agattgcaga tcaacgccaa
     9901 ataatgtctt tacatcttcc ctgagattat agaatgccgt atcaacaaga gcctcgatcg
     9961 tttgtttatc aacaatttta ataaactctt cgtatacttg cttttgcgtc ttatggctgc
    10021 ccagcgccca tgaatactca tgttctttta aaaccagtat ggctcttaac agacaatatc
    10081 catcgccatc tactggagcg tattttaatc catatgtttt attaatttca ctctgaataa
    10141 aagatatgcc agacaaatca ataccagtat ctaaatcaga tattcccggc atatttatct
    10201 gttctggctg gatactcata ttttcattat ctaaacaata ctcagaaaaa acatcatcac
    10261 acgcattaat gttttcaata tattccataa agccatacca ataataacct ttaaaatctg
    10321 tgatagactc tgaacctctg cccggcaagg acactctaac aatatcaaca tcttgaacat
    10381 attgtatagt atatgtaata cctcctgaaa cccctgaata aatttgtgta tcattagtac
    10441 gcaatgttct cataaataat gatagatcat tatcattctg catttcctca aattcttttt
    10501 ctagtgattc acattccaga catggagtat cctcttcatt tactttacgt ctgagttcat
    10561 tgtacaaccg cataaaacac accagtgtgt tttcaaaaag cgtgattgaa tatgtttgat
    10621 ttttttcgta ctcatttttt atatcgttcc aggcctgttt caaataatta tttgcagtgc
    10681 aactaattcc atgtatatta ctacccatat atagcgtcta taaaatttaa taaataatga
    10741 cgccctagtt aaacttaaag tgcctggttc aactatcaaa aatcgctcac cctttttcac
    10801 ctgtttaaaa tatgttcagc aacccatctt gatggcgacc tcctctccgc gatgatttca
    10861 ataacatatt ctgtgttggc atatgaaatt ttgaggatta ccctacactt ataggagtta
    10921 ccttacaggg gttccttcaa tttgtgttga tttacgcgag ataacgctat ggaattaaag
    10981 gattattacg ccatcatggg cgtgaaaccg acggacgatc tcaagacaat caagaccgcc
    11041 tatcgtcgac tggcccgtaa ataccatcct gatgtcagca aagaaccgga tgccgaagcc
    11101 cgcttcaaag aggtcgctga agcctgggaa gtgttaagtg atgaacaacg tcgcgctgag
    11161 tatgatcaga tgtggcaaca tcgcaacgat ccgcaattta accgtcagtt ccatcatggc
    11221 gacggtcaga gttttaatgc cgaagatttt gacgatatct tctcgtcaat tttcggtcag
    11281 catgcccgcc agagccgtca acgccccgcc gcacgtggcc acgatattga aatcgaagtg
    11341 gcggtattcc tcgaagaaac gcttactgag cataagcgta ccatcagcta taacctgccg
    11401 gtttataacg cctttggcat gatcgaacag gaaatcccga aaacgctgaa tgtgaagatc
    11461 ccggcaggcg tcggcaatgg tcaacgcatc cgcctgaaag gccaggggac gccgggcgaa
    11521 aacggcggtc caaatggcga tttgtggctg gtgattcata ttgcgccaca tccgctgttc
    11581 gatattgtcg gccaggatct ggaaattgtg gtgccggtta gcccgtggga agcggcgctg
    11641 ggtactaaag tcaccgttcc aacactgaaa gaaagcattt tgctgactat cccgccaggc
    11701 agccaggccg ggcaacgatt gcgcgttaaa ggcaaaggtc tggtgagcaa aaaacagacc
    11761 ggcgatctgt atgcggtact gaaaatcgtg atgccgccga aaccggatga aaacactgcc
    11821 gcgctgtggc agcaactggc agacgcccag tcgtcttttg atccacgtaa agattggggg
    11881 aaagcataat ggctaatgtt acggtgactt ttactattac cgaattttgc ctgcataccg
    11941 gcatctctga agaggagttg aatgaaattg tcggtttggg ggtggttgaa ccgcgtgaga
    12001 tccaggaaac aacctgggta tttgacgacc atgccgccat tgtggtgcaa cgcgcggtac
    12061 gcctgcgtca tgaactggct ctggactggc cggggatcgc ggtggcgctg acgttaatgg
    12121 atgatattgc gcacctgaag caggaaaacc gcctgctacg ccagcggctt tcccggtttg
    12181 tagctcatcc gtgatgtgtg tttgccggat gtgatgacgc atccggcaac tgcgccataa
    12241 tgcctgatgc gacgctaaac gcgtcttttc aggcgcataa acgacgttat ctgttttggt
    12301 ggtcgcactc cactaacacc agcaataact ggcttagtgc cgcgtaaaaa ccaaagctgt
    12361 catactgtcg acaacgcgca gcaaactctg gtaaccattg ccgcagcgcc gtcagtgttt
    12421 tttgccgcaa actgtcgatt cttcgcgcag gaacggtccc ctctcccagc gaaaaatgca
    12481 gataactcaa caactcgaga tagatcgcca gatgatctgc cggttcgttg aaattgccac
    12541 tggtttccat ccctgcctca actaacaagc gtttaatctc ttgctcgtcc tgtttgtagg
    12601 ccgatgcata cggcagcgcc gcttgtttgt cggtcatcag aaacaggccg caaaagtcag
    12661 cggccagttc cagacgggca tcgtcacgta ctgtcagcgt g
//
LOCUS       DAJTQQ010000101        12646 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000101 DAJTQQ010000000
VERSION     DAJTQQ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12646
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.101"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..1013
                     /gene="ldtD"
                     /locus_tag="OO309_003280"
     CDS             <1..1013
                     /gene="ldtD"
                     /locus_tag="OO309_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5848313.1"
                     /translation="TDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVE
                     AVKRFQAWQGLGADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNI
                     PAYSLLYYQNGNQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPK
                     VRNDPGYLESHGYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYK
                     FNMPSSEAIYLHDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDA
                     LKQGDTRYVNIRQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQL
                     IR"
     gene            1194..1742
                     /locus_tag="OO309_003281"
     CDS             1194..1742
                     /locus_tag="OO309_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR5848314.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            1769..2416
                     /gene="gloC"
                     /locus_tag="OO309_003282"
     CDS             1769..2416
                     /gene="gloC"
                     /locus_tag="OO309_003282"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR5848315.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(2639..3829)
                     /gene="aspC"
                     /locus_tag="OO309_003283"
     CDS             complement(2639..3829)
                     /gene="aspC"
                     /locus_tag="OO309_003283"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR5848316.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(4014..5102)
                     /gene="ompF"
                     /locus_tag="OO309_003284"
     CDS             complement(4014..5102)
                     /gene="ompF"
                     /locus_tag="OO309_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR5848317.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(5703..7103)
                     /gene="asnS"
                     /locus_tag="OO309_003285"
     CDS             complement(5703..7103)
                     /gene="asnS"
                     /locus_tag="OO309_003285"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR5848318.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(7272..8474)
                     /gene="pncB"
                     /locus_tag="OO309_003286"
     CDS             complement(7272..8474)
                     /gene="pncB"
                     /locus_tag="OO309_003286"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR5848319.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8740..11355
                     /gene="pepN"
                     /locus_tag="OO309_003287"
     CDS             8740..11355
                     /gene="pepN"
                     /locus_tag="OO309_003287"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR5848320.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(11562..12329)
                     /gene="ssuB"
                     /locus_tag="OO309_003288"
     CDS             complement(11562..12329)
                     /gene="ssuB"
                     /locus_tag="OO309_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5848321.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(12326..>12646)
                     /locus_tag="OO309_003289"
     CDS             complement(12326..>12646)
                     /locus_tag="OO309_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5848322.1"
                     /translation="ARSYGLSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAET
                     ISANSGIGYLAMNAREFLQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLK
                     EATI"
BASE COUNT         3214 a         3223 c         3179 g         3030 t
ORIGIN      
        1 caactgacgc ggtagtcagc ccatccgctg ttactgttga aacagcagaa actaagccga
       61 tggacaagca aacgacgtct cgtagtaaac ctgcgcctgc cgttcgtgcc gcctacgata
      121 atgaactggt ggaagccgtt aaacgttttc aggcatggca aggattggga gcagatggtg
      181 ctattggccc ggcaacgcgt gactggttaa acgtaacgcc cgcccagcgt gctggcgtgt
      241 tggcactcaa catccagcga ttgcgcttgc tgccaacaga gctttctacc gggatcatgg
      301 ttaacattcc agcctattcg ctgctctact atcagaacgg caatcaggtg ctggattcgc
      361 gagtcattgt cggtcgcccc gatcgcaaaa cgccgatgat gagcagtgca cttaacaacg
      421 tagtggtaaa cccgccgtgg aacgtaccac caactctggc acgcaaagat attctgccga
      481 aagtgcgcaa cgatccggga tatctcgaaa gccatggcta tacggtgatg cgcggctgga
      541 acagcagaga agcgattgac ccatgggagg ttgactggtc tacaatcacg gcctcgaatt
      601 taccgtttcg cttccagcag gctccaggcc cacggaactc gctggggcgc tataaattca
      661 atatgccgag ttcagaggcc atttatttgc atgacacgcc gaaccacaat ctgttcaagc
      721 gtgatacacg cgcattgagc tcaggctgtg tacgagtgaa taaagcttcc gatctggcga
      781 atatgctgtt gcaggatgca ggatggaatg acaaacgtat ttctgatgcg ctgaagcagg
      841 gtgatacacg ttacgtcaat attcggcagt cgattccggt gaatctctac tacctgacgg
      901 cctttgttgg tgcagatgat cgtacccagt atcgtacaga tatttacaat tatgatctgc
      961 ctgcgcgatc cagctcgcaa atcgtatcga aagcggaaca attaatcagg taaatgaagt
     1021 agttcgggga aatcaattgt cgtaataatt agcatgattg ggggcgattc tctgcagccc
     1081 ccgtcactgc tggggttgag tcatcttgac gtctgcttta cgggcggtta aggtgcctct
     1141 tgtgcgccag aagtgcatat aaacgataac attgagctgt agacttgatt atcatggaca
     1201 aattcgacgc taatcgccgc aaattgctgg cgcttggtgg cgttgcactc ggtgccgcca
     1261 tcctgccgac ccctgcgttt gcaacactct ctaccccacg cccgcgcatt ttgacactca
     1321 ataatcttca taccggagag tcaatcaaag cggagttttt cgatggcaga ggctatattc
     1381 aggaagaatt ggcaaaactt aaccattttt tccgcgatta ccgcgcgaac aaaataaagt
     1441 ccatcgaccc aggattattc gaccagttgt atcgcctgca agggttgtta ggcacgcgca
     1501 aaccggtgca actcatttcc ggttatcgtt ccattgatac caacaatgaa ctacgcgccc
     1561 gcagccgtgg agtagcgaag aaaagctatc acactaaagg ccaggcgatg gatttccata
     1621 ttgaaggtat cgcgttaagc aatattcgca aagccgcgtt atctatgcgc gcaggtggtg
     1681 taggatatta cccacgtagt aactttgtgc atattgatac cgggccagca cggcactggt
     1741 agtaatcgct taacgaaaca ggggcagtat gaactatcgt attattccgg tcaccgcatt
     1801 ctcccagaac tgttcattga tctggtgtga acaaacccgc ctggccgcac tggtcgatcc
     1861 tggcggcgat gcggaaaaaa tcaaacagga agttgatgac agcggcctga cactgatgca
     1921 gatcctgctg acgcatggtc atctggacca cgttggcgca gcggcggaac tggcgcaaca
     1981 ttacggcgtg ccggtttttg gcccggaaaa agaagatgag ttctggctgc aaggcttgcc
     2041 tgcgcaaagt cgtatgtttg gtctggaaga gtgccagccg ctgacgccag atcgttggct
     2101 gaacgaaggc gataccatca gcatagggaa tgtgacttta caggtgttac attgccctgg
     2161 gcatacgccg ggtcatgtcg tgttttttga tgatcgggca aagctgctga tttctggcga
     2221 tgttattttc aaaggcggag tagggcgcag tgacttcccg cgtggcgatc ataatcaact
     2281 gatttcttca atcaaagata aattgctgcc actgggggat gacgtgacat ttattccggg
     2341 tcacggacca ttatctacac ttggttatga gcgcctgcac aatcccttcc tgcaagacga
     2401 aatgcccgtc tggtaaaggc gcataaaaaa gtccactttt aatgcgggcc tggatttctg
     2461 gcaaagtgcg ctttgtttat gccggatgcg gcacgagcgc cttatccggc ctacaaaatc
     2521 gtgcaaattc aaaatattgc aggggacgcg taggcctgat aagcgtagcg catcaggcaa
     2581 tgttgcgttt gtcatcagtc tcagcccgct tttcagcggg cttcattgtt tttaacgctt
     2641 acagcactgc cacaatcgat tcgcacagcg gagccatgtt atctggtgtc atcccggcca
     2701 catttacgcg accagaagca acagcatata cgccaaactc ttcgcgcaga cgcagcactt
     2761 gttcttttgt cagaccactg aaggagaaca tgccgttctg tttgatgata aagctgaagt
     2821 cgcggtttgc acctttttcc tgcagcgtat tgacgaacaa ctgacgcata cgctgaatac
     2881 gctggcgcat atcagtcagc tcttgttccc aaatcgcacg taatgcatcg ctgctcagga
     2941 tggtggcaac aacagaagcg ccgtgtgctg gagggttaga gtagttagcg cgaatcgccg
     3001 ctttcatttg gctgaatgcg cgatcaacga tttcactgtc ggcagcaacc agagtacaag
     3061 cgccaacacg ctcgttgtac aggccaaagt ttttagagta ggaactggca acaatcagct
     3121 ctttatgcat agccgcgaaa gcgcgcagtc cttcagcatc ttcttccaga ccacgggcaa
     3181 aaccctggta agcgaagtca aacagcggta accagccttt ctcaacggag agttgtgcca
     3241 gtgtctgcca ttgttccagc gtagggtcga taccggttgg gttatggcag cagccatgga
     3301 acagcactac gtcgccagcc tgagcttcgt tcagactgtt aatcagtgca tcgaagtcaa
     3361 gggtgtgatt ttccgcatca taataagcgt attcacgaac ttccagacct gcagagttaa
     3421 agacgctctt atggttcggc cagcttgggt tgctcaccca cacacgctta acgctggtat
     3481 tttttgccag gaagtcggca gccacgcgta gtgcgccagt gccacccgga gtctgtgccg
     3541 tgcgagcacg tttgtcattg atcagggcgc tacctttacc aaacagcagt tcctgagtgc
     3601 agcgaccaaa ttcaaggatg ccgtcaatgc cgaggtaatt tttggtggtt tcattttcga
     3661 gcagatactg ttcagccttt ttcacgctgg tcagtaccgg ggttttgccc gtctcatctt
     3721 tatagacacc aatcccgagg ttaattttgc cgggacgttc atcggcacga aacagatcgg
     3781 ccaggcccag aatcgggtcg gcaggagcgg cggtaatgtt ctcaaacatg acgaggttcc
     3841 attatggtta cagaagggaa gtccgctatc agggtaacgg gagatttaca aaattccaac
     3901 tattactgat gaaaacgcag gctgtttttg caagacgtga gattgctctg gaaggcataa
     3961 aaaaaacagg accaacgtcc tgttttttcg gcatttaaca aagaggtgtg ctattagaac
     4021 tggtaaacga tacccacagc aacggtgtcg tctgaaccta cgcccagttt gttgtcagaa
     4081 tcgatctggt tgatgatgta gtcaacatag gtggacatgt ttttgttgaa gtagtaggtt
     4141 gcgcccactt caaagtagtt caccagatca acatcaccga taccttctac gtctttcgct
     4201 ttagatttgg tgtaagcgat ggacggacgc agaccgaaat cgaactggta ttgcgcaact
     4261 aacagaacgt cttgcgtttt gttggcgaag ccgctggtgt ttgtaaattt attagtgatc
     4321 ggcgtagcgt tacgggtttc accgtagttc gctgccaggt agatgttgtt cgcgtcgtac
     4381 ttcagaccag tagcccactg ttcagctttt ttaccgttgc caagaggttg agcttcttgc
     4441 aggttggtac ggtcagctgc accataagca ccaacgatac caaagccttc gtattcgtag
     4501 ctgatagaac cgccaacacc gtcgccgtta gaacggcgtg cagtgtcacg ctcgttttta
     4561 cccaggtact gaacagcgaa gttcaggcca tcaaccagac caaagaagtt ggagttacga
     4621 taggtagcaa cgccgccaac acgaccaacg aagaagtcat cgctgtatgc agtatcacca
     4681 ccaaattctg gcagcatatc ggtgtaaccc agtgcatcat aaaccacacc gtagttacgg
     4741 ccgtaatcga aagaaccaac gtcagcgtat ttaagacccg cgaatgccag acgcgttttg
     4801 ttaccagttt gagcgtcagc gccttcagag ttgttaccct ggaagttata ttcccactga
     4861 ccataaccgg tcagatcgga attgatttga gtttcccctt taaaaccaag acgggcatag
     4921 gtcatgtcgc cattgccacc gtaactgttt tcaccgttac ccttggaaaa ataatgcaga
     4981 ccaacagttt taccgtacag atctactttg ttgccatctt tgttatagat ttctgcagcg
     5041 tttgcagtac ctgctactaa cagagcaggg acgatcactg ccagaatatt gcgcttcatc
     5101 attatttatt accctcatgg tttttttatg acacctgcca ctgccgtcaa taagttctgt
     5161 caataaaaat ttacggaact attgatgaga gtttggtgtc tttatgtgtc tgcaggcatc
     5221 tttccattca aactaacgtt tcgctaccgt gaaagtgcta caaagataaa gatttagttt
     5281 caaaaagaaa aaatatgtaa ccaaaagtaa aaattaagga gctttgtgaa caccgtcata
     5341 tttccatagg aacgtgatga tatttacagc aatttttatc tatttatatg atttacttat
     5401 atttaaatta actaaacgga aattttgttt ctgatggaaa ctttatcgac ctggcacaaa
     5461 atcctctttc agacatccag aatgccaaaa aatagtatga aaattgtgct atcagctaag
     5521 aaaaaactaa tccgcaataa gagtacgggt tattttttgt gcgtaaatgt tgcaatgtaa
     5581 tattccgtaa caggatgatc gttatggcgg gggtgcaaat agattgcttg acttaacaaa
     5641 cagataactt gacagaaagg ataaaaaaga gccagcaggc gctggctctt tgaggaagaa
     5701 aatcagaagc tggcgttgcg cggagtacgt gggaacggaa tcacatcacg cacgttttgc
     5761 acgccagtta cgtaagcaat caggcgttca aaaccaagac cgaaacctga atgcggaaca
     5821 gtaccgtagc gacgcagatc gcgataccac cagtaatctt ctttattcag gcccatttcc
     5881 agcatacgct cgtccagaac gtccagacgt tcttcacgct gggagccacc aatgatctca
     5941 ccgatgcccg gagccagaac gtccatagcc gcaacggttt taccgtcttc gttgaggcgc
     6001 atatagaacg ctttaatatc tttcggatag tttttaacca ctaccggtgc tttaaagtgt
     6061 tcttccgcca gataacgctc atgctcagaa gagagatcga cgccccagta aaccgggttt
     6121 tcaaacttcc tgccgcagtt ttcgagaatg gtcactgcgt cggtgtaatc cacctgcgcg
     6181 aaatcggctt caatgaagcg ttccagacgt gaaacggcat ctttatctac gcgttcagcg
     6241 aagaatttca tgtcgtctgc gcgttcttcg agaaccgctt tgaagacata tttcagcatg
     6301 gcttcagcca gacccgcgat atcgttcagg ttagcaaacg ccacttccgg ctccagcatc
     6361 cagaattccg ccaggtgacg gctggtgttg gagttttcag cacggaaagt cgggccgaag
     6421 gtataaattt tggataatgc gcaagcgtag gtttcgccgt tcaactggcc agatacggtc
     6481 aggaaagact ctttaccaaa gaagtctttg tcgaaatcca ctttgccctg atcgttacgc
     6541 ggcaggtttt ccagatccag cgtagaaacg cggaacattt cgcctgcacc ttcggtatca
     6601 gatgcggtaa tcagtggcgt tgaaacccag aagaatccct gctcgttaaa gaagcgatgc
     6661 agcgcctgcg ccagcgtatg gcgaacgcgc gcgacggcac caatcaggtt tgtgcgcgga
     6721 cgcaggtgag cgacttcacg cagatactca atgctgtggc gttttgccgc catcgggtaa
     6781 gtgtctggat cttcaaccca accagcaact tcaaccttgc tggcctgaat ttcaaattgt
     6841 tgcccctggc ccggcgatgc cacgacttta cccgtcacaa tgaccgagca gccggtggtc
     6901 agacgcagga cgtcttcatt gtaattgggc agagaattat tgatgacagc ctgtacagga
     6961 tcaaagcagg aaccgtcata aacggcgagg aaggagatgc cagcttttga atctcggcgg
     7021 gtacgtaccc atccgcgtac ggtgacttcg ctgtcaacgg ctacgcggcc ctggagtacg
     7081 tcggctacag gcacaacgct cataatattc tctctgttaa tagtcggaaa aaataaacac
     7141 ttgtccaccc gtaaatgggg gggataccta tgttacctgg catctgcaat cagacaagca
     7201 gaattcgcaa atgcagcgaa agatttcgga attaaaggta aaaaagggag ccgattagct
     7261 ccctgatgat attaactggc ttttttaata tgcggaaggt cgaacgcttt gcgcagcgcc
     7321 cgaacaaacg ctttatcatg gcagatagtt ttgccagggc tgtcagaaag tttcgccacc
     7381 ggtttaccgt tacactctac caacttaatc acaatattca ggggttttac ctgggggata
     7441 tcgcaggtca ggcgagtccc aataccaaaa cttaattgca cgcgggaaga gaagtggcgg
     7501 tatagctcaa ccgctttgcg taaatccaga ttgtcagaga aaaccagcgt tttactctgt
     7561 ggatcaattc ccagcttttc ataatgtgca atggcttttt caccccattc aaccgggtcg
     7621 ccagagtcat gacgcaggcc ctgataccga ctagcgaact cgacaccgaa atcacgcagg
     7681 aaagcatcca tagtgatgca gtcggttaat gcaatgccaa gttggtcggg atactcttcc
     7741 agccaggcag caagtgcagc tcgctggctg ttggctagat ccgggctgat ttgctgatgt
     7801 gcctggaacc attcgtgtgc ctgtgttccc atcggcgtga gggaaagccg acgcgccaga
     7861 tcgtagttgc tggtgcccac gaaccaggat tcctgttgca gacgcttaac gatggtttct
     7921 tgtacttcgc gagaaaaacg gcgacgggtg ccaaaatcca tcagatggaa gcgcgacata
     7981 tcaagaccgg cggttaacgc cgagaagtcg actaatttgc tttccagcgt gtcgagggct
     8041 tgcgcaacgt cggcctgcgg tgagcgatag cgatgtacca tttcactgat aaccgccagc
     8101 aaaggaactt cccagaggat gacttcacgc cacggaccgc ttaagcgaat atccagcttg
     8161 ccattatcgt tggacacggt gacttgttcc gggttaaagc ggaactcgcg taaccagtta
     8221 agatagtcgg ccttaaagaa aggcagggca gagagccact gatattcatc atcctgcagg
     8281 cgcaggtgct gcatcgcctg aatctgttca cgaatagcat cggcataaat acccagcaga
     8341 tcgtcacctc ggcaacgaaa ctccgccgcg acatgcacat cgtaatagtg atgaaacacg
     8401 gcttgctgca tatgcaactt ataagcatct gtatccagca acgagtgcag aacaggagaa
     8461 gcgaattgtg tcataggtgc gcagtagcgt cctcgtacag gagcgtttag tacaataaac
     8521 atcttcagga actgctggag tataccttgt ttcgcaattt attgaacccc gatcacacca
     8581 tatgccacct ttctggtcga tggcattacg cggtgtatgt tataaaaatg tagcaataaa
     8641 ggcgtttgta cctgaaaaga tgaagattct gcatagcgcg atttacgcaa caggaataga
     8701 ctgaacacca gactctataa aagatgctaa aggttattta tgactcaaca gccacaagcc
     8761 aaataccgtc acgattatcg tgcgccggat taccagatta ctgatattga cttgaccttt
     8821 gacctcgacg cgcaaaagac ggtcgttacc gcggtcagcc aggctgtccg tcatggtgca
     8881 tcagatgctc cgcttcgtct caacggcgaa gacctcaaac tggtttctgt tcatattaat
     8941 gatgagccgt ggaccgcctg gaaagaagaa gaagagggcg cactggtcat cagtaatttg
     9001 ccggagcgtt ttacgcttaa gatcattaat gaaataagcc tggcggcgaa tacggcgctg
     9061 gaagggcttt atcagtcagg cgatgcgctt tgcacccagt gtgaagccga aggtttccgc
     9121 catattacgt attatctcga ccgcccggac gtgctggcgc gttttaccac caaaattatt
     9181 gccgataaaa tcaaatatcc cttcctgctt tccaatggta accgcgttgc gcaaggcgaa
     9241 ctcgaaaacg gacgccattg ggtacagtgg caggacccgt tcccgaaacc gtgctacctg
     9301 tttgcgctgg tggcaggcga ctttgatgta ctgcgcgata cctttaccac gcgttctggt
     9361 cgcgaagtag cactggagct gtacgtcgat cgcggcaacc ttgatcgcgc gccgtgggcg
     9421 atgacctcgc tgaaaaactc catgaaatgg gatgaagaac gctttggcct ggagtatgac
     9481 ctcgacatct atatgatcgt cgcggtggat ttcttcaata tgggcgcaat ggagaataag
     9541 ggtctgaata tctttaactc caaatatgtg ctggcccgca ccgacaccgc caccgacaaa
     9601 gattacctcg atattgaacg cgttatcggc catgaatatt tccataactg gaccggtaac
     9661 cgagtgacct gccgcgactg gttccagctc agcctgaaag aaggtttaac cgtcttccgc
     9721 gatcaggaat tcagctctga ccttggttcc cgcgcagtta accgcatcaa taatgtgcgc
     9781 accatgcgcg gattgcagtt tgcggaagac gccagcccga tggcgcaccc aatccgcccg
     9841 gacatggtca ttgagatgaa caacttctac accctgaccg tttacgagaa gggcgcggaa
     9901 gtgatccgta tgatccacac cctgctgggc gaagaaaact tccagaaagg gatgcaactc
     9961 tatttcgagc gtcatgatgg tagcgcagcg acctgtgacg actttgtgca ggcgatggaa
    10021 gatgcgtcga atgttgatct ctcccatttc cgccgttggt acagccagtc cggtacgccg
    10081 gttgtgaccg tcaaagacga ctacaatccg gaaactgagc agtacaccct gaccatcagc
    10141 cagcgcacgc cagccacgcc ggatcaggca gaaaaacagc cgctgcatat tccgtttgcc
    10201 atcgaattgt atgacaacga aggtaaagtg atcccgttgc agaaaggcgg tcatccggtg
    10261 aattccgtgc tgaacgtcac ccaggcggaa cagacctttg tttttgataa tgtctacttc
    10321 cagccggtgc ctgcgctgct gtgcgaattc tctgcgccag tgaaactgga atataagtgg
    10381 agcgatcagc aactgacctt cctgatgcgt catgcgcgta atggtttctc ccgttgggat
    10441 gcggcgcaaa gtttgctggc aacctacatc aagctgaacg tcgcccgtca tcagcaaggg
    10501 cagccgctgt ctctgccggt acatgtggct gacgctttcc gcgcggtgct gctcgatgag
    10561 aagattgatc cggcgctggc ggcagaaatc ctgacgctgc cttctgtcaa tgaaatggct
    10621 gaactgttcg atatcataga cccgattgct attgccgaag tacgcgaagc actcactcgt
    10681 actctggcga ctgaactggc ggatgagctg ctggctattt acaacgcgaa ttaccagagc
    10741 gagtaccgtg ttgagcatga agatattgca aaacgcactc tgcgtaatgc ctgcctgcgc
    10801 ttccttgctt ttggtgaaac gcatctggct gacgtgctgg tgagcaagca ataccatgaa
    10861 gcaaacaata tgaccgatgc gctggcagcg ctttctgcgg cggttgctgc acagctgcct
    10921 tgccgtgacg cgctgatgca ggagtacgac gataagtggc atcaggacgg tctggtgatg
    10981 gataaatggt ttatcctgca agccaccagc ccggcggcga atgtgctgga gacggtgcgt
    11041 ggtctgttgc agcatcgctc atttaccatg agcaacccga accgtattcg ttcgttgatt
    11101 ggtgcgtttg cgggcagcaa cccggcagcg ttccatgccg aagatggcag cggttaccag
    11161 ttcctggtgg aaatgcttac cgacctcaac agccgtaacc cgcaggtagc ttcacgtctg
    11221 attgaaccgc tgattcgcct gaaacgttat gatgccaaac gtcaggagaa aatgcgcgcg
    11281 gcgctggaac agttgaaagg gctggaaaat ctctctggcg atctttacga gaagataact
    11341 aaagcactgg cttgatactt ttgagccaaa atgcctgatg cgacgcttga cgcgtcttat
    11401 caggcctaca actccgttga aattgctggt tttgtaggct ggataaggcg ttcacgccgc
    11461 atccggcagc cgtgcccaac tgcctgatgc gatgcttgac gtgtcttatc aggcctacaa
    11521 gtccgttgaa aatgctggtt ttgtgcgcca catccggcat attaaccatg acgccgaatc
    11581 agttgttcag aatgcccacg ccgcattacc cgttgcaata cttccgcttc cagctccgct
    11641 aatcgcaccg accctaatcg acgcggacgg gggatatcca ctgtcaaatc cagactaatt
    11701 tttccctctt caattaacaa cacccggtca gccattgcta ccgcttcgct cacatcatgc
    11761 gtcaccaaca gtacggtaaa gccgtgctgc tgccaaagtg acacaatcaa atcctgcatc
    11821 tcgagtcgcg ttaaggcgtc cagtgccccg agcggttcat caagcaacaa taaacccggt
    11881 cgatgaatca acgctcttgc cagcgccaca cgctgtttct gcccgccaga aagtgcggca
    11941 ggccattccc cggcgcgatt ctccagtcct gccgcagcca acgcccgacg cgcggcatcg
    12001 cgccactgac ctttaaggcc taacccaacg ttatcaatca ccgatttcca cggcagcaga
    12061 cgcgcatcct gaaacatcat tcgcgtatca tcctgaattt cagccagcgg tgtggtgccc
    12121 gctaacacat cgcctgcggt tggcgtttcc agaccggcca gcaggtgcag cagggtactt
    12181 ttaccaccac cgctgcggcc caccaccgcc acaaactgac ctgccggaat atgtaaatcc
    12241 agttggttca ggacgatatt ttccgcgtaa tgtttgctta ctgcattgag caacaatggt
    12301 gtgccctggt tcagacgagc agtattcata tcgtggcctc cttcaaatga taagccgggt
    12361 tccagcgcaa ccagaggcgc tctaacagct gcgcgctgac gtcagcaagt ttgccgagca
    12421 gggcgtaaag aataatagcg actaccacca cgtctgtttg caaaaactct cgcgcattca
    12481 tcgccagata accaatgccg gaattggcag aaatggtttc ggcaacaatc agcgtcagcc
    12541 acatcaggcc caacgcaaaa cgcacgccga ccataattga gggcagggca tcgggcagga
    12601 tcacatggat aaacagcggt atgccggata atccatagct acgcgc
//
LOCUS       DAJTQQ010000102        12566 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000102 DAJTQQ010000000
VERSION     DAJTQQ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.102"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(136..855)
                     /locus_tag="OO309_003290"
                     /pseudo
     CDS             complement(136..855)
                     /locus_tag="OO309_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(871..1203)
                     /gene="cusF"
                     /locus_tag="OO309_003291"
     CDS             complement(871..1203)
                     /gene="cusF"
                     /locus_tag="OO309_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCR5848323.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1227..2609)
                     /gene="cusC"
                     /locus_tag="OO309_003292"
     CDS             complement(1227..2609)
                     /gene="cusC"
                     /locus_tag="OO309_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR5848324.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            2766..3449
                     /gene="cusR"
                     /locus_tag="OO309_003293"
     CDS             2766..3449
                     /gene="cusR"
                     /locus_tag="OO309_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR5848325.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3439..4881
                     /gene="cusS"
                     /locus_tag="OO309_003294"
     CDS             3439..4881
                     /gene="cusS"
                     /locus_tag="OO309_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR5848326.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5031..7268
                     /gene="nrfB"
                     /locus_tag="OO309_003295"
     CDS             5031..7268
                     /gene="nrfB"
                     /locus_tag="OO309_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCR5848327.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7255..10223
                     /gene="nfrA"
                     /locus_tag="OO309_003296"
                     /pseudo
     CDS             7255..10223
                     /gene="nfrA"
                     /locus_tag="OO309_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10224..11114
                     /locus_tag="OO309_003297"
     CDS             10224..11114
                     /locus_tag="OO309_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR5848328.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11297..12058
                     /gene="envY"
                     /locus_tag="OO309_003298"
     CDS             11297..12058
                     /gene="envY"
                     /locus_tag="OO309_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR5848329.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12104..12180)
                     /locus_tag="OO309_003299"
     tRNA            complement(12104..12180)
                     /locus_tag="OO309_003299"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12144..12146),aa:Arg,seq:tct)
     gene            12423..>12566
                     /locus_tag="OO309_003300"
     CDS             12423..>12566
                     /locus_tag="OO309_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="activates the production of the major fimbrae
                     protein FimA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbriae biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HCR5848330.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTR"
BASE COUNT         3066 a         3273 c         3199 g         3028 t
ORIGIN      
        1 gcgcacagta aacaactata tttgacgttc gtagacatac tcaggtgaaa aaaaggttgt
       61 ttttcgagtt ataacagaca gttaattaac tacagtggac aatgctagac aatgacagac
      121 acagataagc aacctacctt cctctttcgc gaccacgtta tctttggcga tattcattcc
      181 cgcgcgcaga tcaaacgcgg tgatcacgcc atcaatgggc gctttgagcg taaagcgagt
      241 ctggattttt tgcgtggcga tcaggtggcg aatatccgcc tccggcattc ccgccagtcg
      301 cagccgctca agaatgcctt cagtctgggt cgccgtaccg ccggtttcgc gcaacagtaa
      361 atactcactc tgcgcttcca cccagtcggg aatggtcagg tcgagaagcg gtgtgccctt
      421 ttgtacttta tcgcccacgg taagcggata caccttgtcg ataaacccgg cagcgcgagc
      481 ctgcacaatg gcatactgat actcgttgta actgacattc gtcgggaaac tctgggcaaa
      541 agtcagcggc ccgcgcgtga cggtagccgt tttcaccccc agattctgcg tctgagtcgg
      601 gtcaatgcgc acaccagacg cagaactctc ttcatcggca tatttcggca ccagatccat
      661 atccataaac ggcgatttac ctggtttatc gaaccgcgta ttgggataca tcgggtcgta
      721 ccagaataag attttacgtt ctgcggtcgg cattttttct gcgtgcggtt ccgcctggtc
      781 aaaccaggta aaacctgccg ctgaaataat accgcccgcg atcatgctgc cgataataag
      841 cgcgattttt ttcatctcat taaacctggg ttactggctg actttaatat cctgtaataa
      901 agaaaggttg ccctgctgga caaaattaaa cgccactttg tcgccggttt taatttcact
      961 cattttcgtc tgcggggtga tggtaaagcg catggtcatc tccggccagt tgacggcagc
     1021 aatcggatcg tgatggatgg tgattttttt gctttccaga tcaacacctt ttaccacacc
     1081 ggtggcacta atgacctgtg gttgtgcttc gctcatggtt tcatgatgat gttcgttagc
     1141 ctgggcatta aagccaataa cggtaaacag actgaacatt gcgacttgca gtgctttttt
     1201 catttattct ctcctggagt taaaaattat tgctgccaac cgccccctaa ggcggtatac
     1261 aaataaattt cgttaacctg acgggcataa ttcaggtcaa gtaaagtttg tcgggttgca
     1321 aataaagaac gctcggcatc cagcacttcc agataactta ccgcgccgtg ctgatataat
     1381 gcccgcgccc gttgcagggt aatttgcagc gacgccagat aacgctgctg ggcgctgatt
     1441 tgatcgtcca ggctttgacg taatgccagc gcatctgcca cctctttaaa ggcgttctgg
     1501 attttctgtt cataattcac caccgactgc tgctggcgaa tttcggcgat atccagattg
     1561 gcctggttgc gtccggcatt aaaaatgggg atctcaattt taggaataaa attccacatc
     1621 ccgctgctag cgttaaataa tgacgacaga tcgctgctgg cggttgatat tccgctggtc
     1681 aggctgatag acgggaaaaa tgccgcacgc gcagcaccaa tattagcatt agccgccatt
     1741 aacgcgtgtt cagcttccat aatatccggg cgctgcaata agatttgcga cgacaagccc
     1801 gccggtaatt taacgctttg caggctgccg ctgtttactg tctgcgcttg cggtagcttg
     1861 ccgtagcttc ccaataacag ttgcaatgca ttattcgcct gcgccagttc cccctgacgt
     1921 ttagcgatgt cactgcgggt actttctatc accccgcgag cctgttccag cgccagaaca
     1981 ttgctgctac cggtcaacag ttgtttttcg acaaacgcat atgactgctg ataattacgc
     2041 agcgtttctt cggctatttg caattgcgca tacgccagct gctgattgaa atagctttgc
     2101 gcgacattag aaaccagcaa aatatgcacc gcgcgctggg cttcctcagt ggctaaataa
     2161 ttttggcgct cggcttcgct catgttcttt aagcgaccga aaaaatcgag atcaaagctg
     2221 gcgttaaggc cagtcgagaa ctcccgcgtc gtggctgaat cgcctttaag attgccgctc
     2281 cagctgccgc tgccctcgcc attgagctgt gggtagcggt cggcatcggt cagacgatat
     2341 tgcgcccgtg cctcctgcac tttcagcgtc gccatgcgca aatcccggtt attcaccagc
     2401 gcctcgctaa tcagcgtctt cacctgatta tcaacaaaaa aggtgcgcca gcccacgttc
     2461 tggtagttat ccgctgcgtt aaccaggccg ttctggctga gtgagaactg ctgcggtacg
     2521 ggcattaccg gacgctgata atccggtgcc agtgaacaac cagttagcgc aagggccaca
     2581 caaaatggca gaagtttaca aggagacata ggctcataat ttctggtgat tttatgtcgc
     2641 caactttact cgccaggctc tgattttccg gtgacaggaa aatgacaaaa ttgtcatttt
     2701 gccaataagc gattgccatc tgatcccgct actctagaat tgcccgggca acatgcggag
     2761 gaaatatgaa actgttgatt gtcgaagatg aaaagaaaac cggagaatac ttgaccaaag
     2821 ggttaaccga agccggtttt gtggtcgatt tggccgacaa cgggctgaat ggctaccatc
     2881 tggcgatgac cggtgattat gacctgataa tcctcgatat tatgctgcca gacgtgaacg
     2941 gctgggatat cgtgcgcatg ttgcgctccg ccaataaagg gatgccgatt ctgttgctta
     3001 ccgcgcttgg caccattgaa catcgcgtca aggggctgga gttgggggca gatgactacc
     3061 tggtgaaacc gttcgctttt gctgaactgc tggcgcgggt gcgcactctc ctgcggcgcg
     3121 gggcggcggt gattatcgaa agtcagtttc aggttgccga tctgatggtc gatctcgtca
     3181 gccgcaaagt cacccgcagc ggcacgcgca tcactctgac cagtaaagaa tttactctgc
     3241 tggagttttt tctccgccat cagggcgaag tgctgccccg ctcgcttatc gcctcgcagg
     3301 tatgggacat gaatttcgac agcgacacta acgccattga tgtggcggtg aagctgctac
     3361 gcggcaaaat cgacaacgac tttgagccga agctgattca aaccgtgcgc ggcgtgggtt
     3421 acatgcttga ggtgccggat ggtcagtaag ccatttcagc gcccgttttc gctggcaacc
     3481 cgcctgacct tttttatcag ccaggccacc atcgcggcgt ttttcgcctt tgcatggatc
     3541 ataatccact cagtaaaagt gcattttgcc gagcaggaca ttaatgattt aaaagagatt
     3601 agcgccaccc ttgaacgggt actaaatcac cctgacgaaa cgcaagcccg acgcttaatg
     3661 acgctggaag atatcgtcag tggttattcc aacgtgttga tttccctggc agatagccac
     3721 ggcaaaacgg tgtatcactc ccccggtgcg cctgatatcc gcgagtttgc acgtgatgcc
     3781 atacccgata aagacgcgcg gggtggcgag gtgtatctcc tttccggccc gacgattatg
     3841 atgccaggcc acggtcacgg gcatatggaa cacagcaact ggcggatgat taacttgccg
     3901 gttggcccgt tggtggacgg caaaccgatt tatacgctct acatcgcgct gtcgatcgat
     3961 tttcatcttc attacataaa tgatttgatg aataaactta ttatgaccgc atcggtaatc
     4021 agcatcctga tcgtctttat cgtgctgttg gcggtacata aaggtcacgc gccgatccgc
     4081 agcgtcagcc gtcaaatcca gaatatcacc tcgaaagatc tcgacgttcg ccttgacccg
     4141 cagaccgtgc cgattgagct ggaacagctg gtactgtcgt tcaaccatat gatcgagcgt
     4201 attgaggatg tctttactcg ccagtccaat ttctcagcgg atatcgccca cgaaattcgc
     4261 acaccaatta ctaatctcat tacgcaaacg gaaatcgccc tcagccagtc gcgcagccag
     4321 aaggagctgg aagatgtgct ctactctaat ctcgaagagc tgacgcgaat ggcgaaaatg
     4381 gtcagcgata tgctgtttct cgctcaggcc gataacaacc agctaatccc cgaaaagaaa
     4441 atgctcaacc tggcggatga agtcggcaaa gtgttcgatt ttttcgaggc gctagcggaa
     4501 gatcgcggcg tggagttgcg gtttgttggc gacaaacgtc aggttgcggg cgatccgttg
     4561 atgctgcgtc gggcgctaag caacctgctt tctaatgccc ttcgttatac gccaaccaga
     4621 gagacaattg tagtacgctg tcagacggtc gatcaccagg tgcaagtttc cgtcgaaaac
     4681 cccggtacgc ccattgcgcc cgagcactta ccgcgattgt ttgaccgttt ctatcgcgtt
     4741 gacccctccc gccagcgaaa aggtgaaggt agcggtattg ggctggcgat agtgaaatcg
     4801 attgttgtcg cgcataaagg cacggttgcg gtaacgtcag atgcgcgggg gacaaggttt
     4861 gttatcacat tacccgctta attattcagc aaacggcaac ttttataacc agtgtaaaaa
     4921 taacgtgccg caatagtcat acatattaat ggtaaaaagc tgtcacaatt cataaaaaac
     4981 cttaatatac gccaccctaa acataaccag cgttaatgta aggtttttgt gtggactggc
     5041 ttcttgatgt ttttgctacc tggctctacg gcttaaaagt aatcgcgata acgttagcgg
     5101 tcatcatgtt catcagcggg ctggacgatt tttttattga tgtcgtctac tgggtacgcc
     5161 gcattaaacg caagttgagt gtttatcgcc gctacccgcg aatgagttac cgcgaactgt
     5221 ataaaccaga tgaaaaaccg ttagcgatta tggttccggc gtggaatgaa acgggcgtca
     5281 tcggcaatat ggccgagctg gcggcgacca cgctcgacta cgaaaactat catatctttg
     5341 ttggcaccta ccccaacgac cccgatactc aacgtgatgt tgacgaagtg tgcgctcgct
     5401 ttccgaacgt gcataaggta gtctgcgcgc gtcctggtcc caccagcaaa gccgactgcc
     5461 tgaacaacgt gctggacgcc atcacccagt ttgaacgtag cgccaatttc gcttttgctg
     5521 gttttattct gcatgacgcc gaagatgtga tttcaccgat ggaattgcgt ctgttcaact
     5581 atctggtcga gcgtaaagat ctgattcaga tcccagtgta tccgtttgaa cgcgaatgga
     5641 cgcacttcac cagcatgact tacattgatg agttttcaga actgcatggc aaagatgttc
     5701 cggtgcgtga agccctcgcc ggacaagtgc ccagcgcagg cgtcggcacc tgtttcagcc
     5761 gccgcgccgt gaccgcactg ttagctgacg gtaacggtat tgctttcgac gtgcagagtc
     5821 ttactgaaga ttacgacatt ggcttccgcc tgaaagaaaa aggtatgacg gaaatttttg
     5881 tccgttttcc ggtggtggac gaagccaaag aacgcgagca gcgtaaattt ttacagcacg
     5941 cacggacgtc aaacatgatc tgcgtgcgcg aatatttccc cgataccttt tcgactgcgg
     6001 ttcgacaaaa atctcgctgg atcatcggca ttgttttcca gggctttaaa acccataaat
     6061 ggacctccag cctgacgctg aactactttc tctggcgcga ccgcaaaggg gcaatcagta
     6121 actttgtcag cttcctcgcg atgctggtga tgatccagct tttgctgttg ctggcgtatg
     6181 aaagtttgtg gcccgatgcc tggcatttcc tttctatttt tagcggcagc gcatggttaa
     6241 tgaccctgct gtggctaaac tttggcttga tggttaaccg catcgtgcag cgagtgattt
     6301 tcgtcactgg ctactacggc ctcacgcagg gcctgctttc cgtcctgcgt cttttctggg
     6361 gcaacctgat taactttatg gctaactggc gcgtgctgaa acaggttctt cagcacggcg
     6421 atccgcgtcg cgtcgcgtgg gataaaacaa cgcatgactt ccccagcgtc actggcgata
     6481 cccgctcgtt gcgcccgtta ggtcaaattc tgctggaaaa tcaggtcatc actgaagaac
     6541 aactcgatac agcactgcgt aatcgcgtcg aaggtctacg cctgggcggt tcaatgctga
     6601 tgcaggggct gattagcgcc gagcagctgg cacaggcgct ggcagagcaa aacggcgtgg
     6661 cgtgggaatc catcgatgcc tggcagatcc cgtcctcgct gattgccgaa atgccagcct
     6721 ccgtagcgct gcattatgca gtactgccgc tgcgtctgga aaatgatgag ttaattgtcg
     6781 gcagtgaaga tggtattgac ccggtttcgc tggcggccct gacgcgtaaa gtcggacgca
     6841 aagtgcgtta cgtcattgtt ctgcggggac aaattgtcac agggttacgc cactggtatg
     6901 cacgccgacg cggtcacgat ccgcgggcaa tgttgtacaa tgcggttcag catcagtggc
     6961 tcacggaaca gcagaccggt gaaatctggc ggcaatatgt gccgcatcag ttcctgttcg
     7021 ccgaaatact gaccacgctc ggtcatatta atcgttcagc aattaacgtg ttgttattgc
     7081 gccatgaacg cagttctctg ccgctcggca agtttttggt caccgaaggc gttatcagcc
     7141 aggaaacgtt ggatcgcgtc ctgacaattc aacgcgaatt acaagtttcg atgcaatcac
     7201 tattactcaa agcaggttta aacacagaac aggttgcgca actggagtcc gaaaatgaag
     7261 gagaataacc ttaatcgcgt catcggatgg tctggtttac tgctgacgtc tttattgagt
     7321 accagcgcac tcgcagacaa tatcggcacc agcgcagaag agctggggct gagcgattat
     7381 cgccattttg ttatttatcc ccgtctcgat aaggcgctga aggcacagaa aaataacgac
     7441 gaagcaaccg ccatccgcga atttgaatat atacaccaac aggtgccgga taatattccg
     7501 ctgactttat accttgcgga agcctatcgt cattttggtc atgatgaccg ggcgcggctg
     7561 ttgcttgagg atcaactgaa acgtcaccca ggagatgccc gacttgagcg cagtctggcg
     7621 gctattccag ttgaagtgaa aagcgttacg actgttgaag aactgcttgc ccagcaaaaa
     7681 gcgggcgacg ctgcgccgac cctgcgttgt cgcagtgaag tcgggcagaa tgccctgcgg
     7741 ctggcacaat tacctgtcgc cagagcgcaa ctgaacgatg cgacgtttgc tgcatcgccg
     7801 gaaggaaaaa cgctgcgaac cgatctgctg caacgggcaa tctacctgaa acaatgatcc
     7861 caggcagata cgctatacaa tgaagcacgc cagcagaaca cattaagcgc ggcagaacac
     7921 cgtcagtggt ttgacgtgct tcttgccggg cagctggacg atcggatcct ggcactgcaa
     7981 tcacagggga tcttcaccga tcctcagtca tatattactt acgcgaccgc gctggcttat
     8041 cgtggcgaaa aagcacgcct ccagcattat ctcattgaaa ataagccgct gtttaccacg
     8101 ggcgcacaag agaaaagttg gctctatctg ttatctaaat acagcgccaa ccccgttcag
     8161 gcattggcga attatacggt gcagtttgcc gataaccgcc agtatgttgt tggcgcgacg
     8221 ctaccggtgc tgttaaaaga aggtcagtac gacgcagcgc aaaaactgct cgccaccctc
     8281 cccgccaatg aaatgcttga ggagcgttat gctgtcagcg tggcgacccg taacaaggct
     8341 gaatctctgc gtctggcacg attgctgtat cagcaagaac cgacaaatct tccccgcctg
     8401 gatcaactga cctggcaact gatgcagaac gagcagtcac gcgaagctgc cgatttattg
     8461 ctgcaacgct atcctttcca gggcgatgcg cgtgtcagcc agactttaat ggcgcgactg
     8521 gcgtctctgc tggaaagtca tccttaccta gcaacgccgg cgaaggtggc gattttatcg
     8581 aaacccttac cgctggcgga gcaacgtcag tggcaaagtc agttgccggg tattgcagat
     8641 aattgcccgg caatagttcg cttgctgggc gatatgtcgc cttcctacga tgctgccgcc
     8701 tggaaccgtc tggcaaagtg ttatcgggac acgctacccg gtgtggcgtt gtatgcatgg
     8761 cttcaggccg aacaacgaca accgagcgcc tggcaacatc gtgcggtagc ctatcaggcg
     8821 tatcaggttg aggactacgc caccgcactg gcggcctggc agaaaatcag tcttcacgac
     8881 atgagcaatg atgatctgct cgccgccgcc aacaccgctc aggcagcggg aaatggcgca
     8941 gctcgcgatc gctggcttca acaggcagaa caacgtgagc tgggaaacaa tgctcaatac
     9001 tggtggctgc atgcgcgacg ttatatccca gctcagccag aactcgcact gaacgatctc
     9061 acgcgctcaa tcaatattgc cccttctgcc aactcttacg ttgcgcgggc gacaatttat
     9121 cgccaacgtc ataatgttcc ggccgcggtg agtgatttgc gcgccgcgct ggaactggaa
     9181 ccaaataata gcaacaccca ggcagcgctt ggttacgcct tgtgggatag cggtgatatc
     9241 gcacagtcgc gggaaatgct cgaacaggcg cataaagggc ttccggacga tccggcactg
     9301 atccgacaac tggcctacgt gaaccagcgt ctggatgaca tgcctgcgac gcagcactac
     9361 gcccggctcg tgattgatga cattgataat caggcataac cccactgacc ccagagcaaa
     9421 atcagcaacg cttcaatttc cgccgtttgc atgaggaggt cggtcgccgc tggacgttca
     9481 gtttcgattc ttccatcggc ttgcgttccg gcgcaatgag taccgctaac aataatgtcg
     9541 gcggtgcagc gcctgggaaa agctatcgta gctacggaca actggaagcc gagtaccgca
     9601 tcggacgcaa tatgctgctg gaaggcgacc tgctctcagt ttatagccgc gtctttgccg
     9661 ataccggaga aaacggggtg atgatgccgg tgaaaaatcc gatgtccggc accggtctgc
     9721 gctggaagcc gctgcgcgat cagatctttt tcctcgccgt cgaacagcag ttgccgctga
     9781 acgggcaaaa tggcgcatcc gataccatgc tgcgcgccag cgcctcattc tttaatggcg
     9841 gcaaatacag cgacgaatgg cacccgaacg gttcaggctg gtttgcccaa aacctgtacc
     9901 tcgatgcggc gcaatatatc cgccaggata ttcaggcgtg gacggcagat tatcgcgtca
     9961 gctggcatca gaaggtagct aacggacaga ctattgagcc ttacgctcac gttcaggaca
    10021 acggctatcg tgataaaggc actcagggcg cgcagcttgg cggtgtcggg gtccgctgga
    10081 atatttggac cggcgagacg cactacgacg cctggccgca caaagtcagt ctcggcgtcg
    10141 agtatcaaca tacctttaag gcgattaatc aacgtaacgg agagcgcaac aacgcgtttc
    10201 tcaccattgg agtgcactgg taaatgcgta agttcatttt cgtattgctg acactgcttt
    10261 tggtcagccc tttttccttt gcgatgaaag gtattatctg gcaaccacaa aaccgagata
    10321 gtcaggttac cgatacccag tggcaggggc tgatgagtca gttacgtttt caaggcttcg
    10381 ataccctggt tttgcaatgg acccgttacg gcgatgcatt tacccagcca gaacagcgca
    10441 cgttattgtt taagcgtgcc gcagctgcgc aacagactgg cctgaagctg attgtcgggc
    10501 tgaacgccga tccggaattt tttatgcacc agaaacagtc gtctgcagcg ctggaaagct
    10561 atcttaatcg cctgctggct gccgatctcc agcaagccag attatggagc gccgcgcctg
    10621 gcataacgcc ggatggctgg tacatcagcg cggaaattga cgacctgaac tggcgcagcg
    10681 aagccgcccg tcaacctttg ctaacatggt taaacaacgc gcagcggctg attagcgatg
    10741 tttcagcaaa accggtttat atcagtagtt ttttcgccgg aaacatgtcg cccgatggct
    10801 atcgccaact gctggaacaa gttaaagcaa ccggagttaa tgtctgggta caggatggca
    10861 gcggcgtgga taaacttacc gctgaacagc gtgaacgtta tctacaggcc agcgccgatt
    10921 gccaaagctc cgccccagca agcggcattg tttatgaact gtttgtcgcc ggcaaaggca
    10981 aaacctttac agcgaaaccg aaaccggacg cagaaattgc ctcgctgtta gcgaaacgtt
    11041 cctcttgcgg taaagacatg ctctatttct ctctgcgcta tttgcccgtc gcgcacggca
    11101 ttctcgagta ttaaatcccc tccaggtaag tcgggtacga cctggcttac ctctttcgct
    11161 ctcgtcagaa ttccatcaag attaattgct aaaaagcatg ctaaagaaag aagatcgtta
    11221 tcggcgaacg ggccgatcac tcatgcagga agcatagatc ctttgagcgg agtttctgat
    11281 atctgaggag tgcgaaatgc aattgagcag cagtgaacct tgcgtggtga tcctgaccga
    11341 aaaagaggta gaggtaagcg tcaataacca tgctacgttt acccttccga aaaactacct
    11401 ggccgccttc gcgtgcaaca ataacgtcat tgaactctca acgttaaatc acgtattaat
    11461 cacccacatc aaccgtaaca tcatcaacga ttatctgttg tttttaaata agaaattaac
    11521 ctgtgtaaag ccctggtcgc ggctagcaac cccggttatc gcttgtcata gccgtacacc
    11581 ggaagtgttc cggctagcca ccaaccacag caagcagcaa tccagcaaac cctgcgaagc
    11641 ggagttgacg cgcgcattac tttttaccgt attgtctaac tttcttgagc aatcgcggtt
    11701 tattgcccta ctgatgtata tcttacgcag cagcgtccgc gacagcgtct gccgcattat
    11761 tcaaagcgat attcagcatt actggaatct gcgaattgtc gccagttcgc tatgtttaag
    11821 ccccagcctg ctcaaaaaga aattaaaaaa cgaaaatacc agctatagcc agattgtcac
    11881 agagtgtcgt atgcgttacg ccgtacagat gttattgatg gataacaaaa atatcactca
    11941 ggtggcgcaa ttatgtggct atagcagcac gtcgtacttt atctctgttt ttaaggcgtt
    12001 ttacagcctg acaccgttga attatctcgc caaacagcga caaaaagtga tgtggtgaag
    12061 ggcaaagcgg aaacggataa gacgggcata aatgaagaag aaatggcgcg ccctgcagga
    12121 ttcgaacctg cggcccacga cttagaaggt cgttgctcta tccaactgag ctaagggcgc
    12181 gttgataccg caatgcggtg taatcgcgtg aattatacgg tcaacccttg ctgagtcaat
    12241 ggcttttgat ctggttgctg aacaagtgaa cgaccgcgtc tgattttctg atttatttcg
    12301 ctatagcggc aaacaaacgc acaccgctgc gcgtctgaat caagaaaacc cgtattttca
    12361 tgtatcaaag tacaatttac cgccctaacg gaaaattgtc cgctcctatg agactggtaa
    12421 ctatgaaacc aacgtcggtg atcattatgg atactcatcc tatcatcaga atgtctattg
    12481 aagttctgtt gcaaaaaaac agtgaattgc agattgtcct gaaaacggat gattaccgca
    12541 taaccatcga ttatctccga acccgt
//
LOCUS       DAJTQQ010000103        12488 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000103 DAJTQQ010000000
VERSION     DAJTQQ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.103"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..1668
                     /gene="maeA"
                     /locus_tag="OO309_003301"
     CDS             <1..1668
                     /gene="maeA"
                     /locus_tag="OO309_003301"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5848331.1"
                     /translation="LYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLGLLPEVVETIE
                     EQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPVIYTPTVGAAC
                     ERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILGLGDQGIGGMG
                     IPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRITDDEYYEFVD
                     EFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAAVTVGTLIAAS
                     RAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFMVDRFGLLTDK
                     MPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQTGLFTEEIIR
                     EMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVVWKDKIYPIAQ
                     CNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLVLPELKDIQKV
                     SRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1802..2812
                     /gene="adhP"
                     /locus_tag="OO309_003302"
     CDS             1802..2812
                     /gene="adhP"
                     /locus_tag="OO309_003302"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR5848332.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            2958..3242
                     /locus_tag="OO309_003303"
     CDS             2958..3242
                     /locus_tag="OO309_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5848333.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3470..4123)
                     /gene="fdnI"
                     /locus_tag="OO309_003304"
     CDS             complement(3470..4123)
                     /gene="fdnI"
                     /locus_tag="OO309_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR5848334.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4116..5000)
                     /gene="fdnH"
                     /locus_tag="OO309_003305"
     CDS             complement(4116..5000)
                     /gene="fdnH"
                     /locus_tag="OO309_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR5848335.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5013..8060)
                     /gene="fdnG"
                     /locus_tag="OO309_003306"
                     /pseudo
     CDS             complement(5013..8060)
                     /gene="fdnG"
                     /locus_tag="OO309_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
     gene            8292..9173
                     /gene="yddG"
                     /locus_tag="OO309_003307"
     CDS             8292..9173
                     /gene="yddG"
                     /locus_tag="OO309_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR5848336.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9428..10528
                     /gene="ompC"
                     /locus_tag="OO309_003308"
     CDS             9428..10528
                     /gene="ompC"
                     /locus_tag="OO309_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5848337.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            10692..11177
                     /locus_tag="OO309_003309"
     CDS             10692..11177
                     /locus_tag="OO309_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5848338.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            11287..12276
                     /locus_tag="OO309_003310"
     CDS             11287..12276
                     /locus_tag="OO309_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5848339.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         3278 a         3095 c         2900 g         3215 t
ORIGIN      
        1 ctttatatcc cttacgctgg ccctgtactg ctggaatttc cgttgttgaa taaaggcagt
       61 gccttcagca tggaagaacg ccgtaacttc aacctgctgg ggttactgcc ggaagtggtc
      121 gaaaccatcg aagaacaagc ggaacgagca tggatccagt atcagggatt caaaaccgaa
      181 atcgacaaac acatctacct gcgtaacatc caggacacca acgaaaccct cttctaccgt
      241 ctggtaaaca atcatcttga tgagatgatg cctgttattt ataccccaac cgtcggcgca
      301 gcctgtgagc gtttttctga gatctaccgc cgttcacgcg gcgtgtttat ctcttaccag
      361 aaccgccaca atatggacga tattctgcaa aacgtgccga accataatat taaagtgatt
      421 gtggtgactg acggtgaacg tattctgggg cttggtgacc agggcatcgg cgggatgggc
      481 attccgatcg gtaaactgtc gctctatacc gcctgtggcg gcatcagccc ggcgtatacc
      541 cttccggtgg tgctggatgt cggaacgaac aaccaacagc tgcttaacga tccgctgtat
      601 atgggctggc gtaatccgcg tatcactgac gatgaatact atgaattcgt tgatgaattt
      661 atccaggctg tgaaacaacg ctggccggac gtgctgttgc agtttgaaga ctttgctcaa
      721 aaaaatgcga tgccgttact taaccgctat cgcaatgaaa tttgttcttt taacgatgac
      781 attcagggca ctgcggcggt aacagtcggc acactgatcg cagccagccg cgcggcaggt
      841 ggtcagttaa gcgagaaaaa aatcgtcttc cttggcgcag gttcagcggg atgcggcatt
      901 gccgaaatga tcatcgccca gacccagcgc gaaggattaa gcgaggaagc agcgcggcag
      961 aaagtcttta tggtcgatcg ctttggcctg ctgacggaca agatgccgaa cctgctgcct
     1021 ttccagacca aactggtgca gaagcgcgaa aacctcagtg actgggatac cgacagcgat
     1081 gtgttgtcac tgctggatgt ggtgcgcaat gtaaaaccag atattctgat tggcgtctca
     1141 ggacagaccg ggctgtttac ggaagagatc atccgtgaga tgcataaaca ctgtccgcgt
     1201 ccgatcgtga tgccgctgtc taacccgacg tctcgcgtgg aagccacacc gcaggacatt
     1261 atcgcctgga ccgaaggtaa cgcgctggtc gccactggca gcccgtttaa tcctgtggta
     1321 tggaaagata aaatctaccc tatcgcccag tgtaacaacg cctttatttt cccgggcatc
     1381 ggcctgggtg ttattgcttc cggcgcgtca cgtatcaccg atgagatgct gatgtcggca
     1441 agtgaaacgc ttgctcagta ttcgccgctg gtcctgaacg gcgaaggtct ggtactaccg
     1501 gaactgaaag atattcagaa agtctcccgc gcaattgcgt ttgcggttgg caaaatggcg
     1561 cagcagcaag gcgtggcggt caaaacgtct gctgaagctt tgcaacaagc cattgacgat
     1621 aatttctggc aagccgaata ccgcgactac cgccgtacct ccatctaagc ctgcacccgg
     1681 tagtgaaggc taccgggcta tttccctctc cctttttcag atctcatcca tactgggtag
     1741 tggcgaataa atctcatttg cctcacctgc tatgcagaac atcatccgaa aaggaggaac
     1801 tatgaagact gcagttgtta cgaaggatca tcatgttgac gttacggata aaacactgcg
     1861 ctcactgaaa catggcgaag ccctgctgaa aatggagtgt tgtggtgtat gtcataccga
     1921 tcttcatgtt aagaatggcg attttggtga caaaaccggc gtaattctgg gccatgaagg
     1981 catcggtgtg gtggcagaag ttggcccagg tgtcacctca ttaaaaccag gcgatcgtgc
     2041 cagcgtggcg tggttttacg aaggatgcgg tcattgcgaa tactgtaaca gtggtaacga
     2101 aacgctctgc cgttcagtta aaaatgccgg atacagcgtt gatggcggga tggaggaaga
     2161 gtgcatcgtg gtcgccgatt acgcggtaaa agtgccagat ggtctggact cggcggcgcc
     2221 cagcagcatt acctgtgcgg gggtcaccac ctacaaagcc gttaaactgt caaaaattcg
     2281 tcccggtcag tggattgcta tctacggtct tggcggtctg ggtaacctcg ccctgcaata
     2341 cgcaaagaat gtctttaacg ccaaagtgat cgccattgat gtcaatgatg agcagttaaa
     2401 actggcaacc gaaatggatg cagatttagc gattaactca cgcaccgaag acgccgccaa
     2461 aattgtgcag gagaaaaccg gtggcgctca cgctgcggtg gtaacagcag tagctaaagc
     2521 tgcgtttaac tcggcagttg atgctgtccg tgcaggcggt cgtgttgtgg ctgtcggtct
     2581 gccgccggag tctatgagcc tggatatccc acgtcttgtg ctggatggca ttgaagtggt
     2641 cggttcgctg gtcggcacgc gccaggatct aaccgaagcc ttccagtttg ccgccgaagg
     2701 caaagtggtg ccgaaagtcg ccctgcgtcc gttagcggac atcaacacca tctttaccga
     2761 gatggaagaa ggcaaaatcc gtggccgtat ggtgattgat ttccgtcact aagaggtctt
     2821 tgctgcgact cccatgttcg ggtcgcagca tcgcacactc tccaacatga aatggctaaa
     2881 tggattattg ttgcgtagga aggaacaata atgatagtgt tcccagagaa gtaacgctac
     2941 tgagagtaaa gacactaatg aaaatggcca atcatccccg cccgggggac attattcagg
     3001 aatcactgga cgaacttaat gtcagcctgc gcgagtttgc cagagcaatg gaaattgcgc
     3061 cctcaacggc aagccgattg ctgaccggaa aagcagcttt gacgccagaa atggcaatta
     3121 aactttccgt ggtgatcggc agttcgccgc aaatgtggct gaatctgcaa aacgcctgga
     3181 gtctggcaga ggcagaaaaa acggtggttg tgtcgcgatt acgccgtctg gttacgcaat
     3241 aaaaaatggc accctataaa gcgccatttg aaactgatga caaacgcaac attgcctgat
     3301 gcgctacgct tatcaggcct acacgttcac ttcaatctat tgtatttgaa gggttttcat
     3361 gccggataag gcattcacga cgcatccggg atgaacaaag cccacgttgt ctacaatatg
     3421 aaaatggcgc cttttacagc gccattttca gcttaaagtt caaaagagat tatatccctt
     3481 cttcactctc ttttttcgct tctgccttct cgatttcacg ataccagcgc ggatggtgtt
     3541 tcttcgccca gcgacggctc accttccctt cgatcatccc tttaatcgac cctttcaccc
     3601 agaatgccat atacatatgg atcaggatgg cgtggatcag gatgataccc gcagccgcgt
     3661 ggatcagcag gctgtagcga acaacctgca tcgggaagta ctgcgcaaag tacggacgcc
     3721 agataatcac cccggtcacc agcagcacga aaatcatgct catgatcgac cagaacatca
     3781 tcttttgccc ggcgttgtac ttaccaacat ccgccacttt gtgctcattg ccttttaata
     3841 cttcgacaat gttcaacagc cacggaatat ctttcttatc cgggatgttg tgatgcacaa
     3901 aacgcacaaa cataaacatc agtgcgacga aaatcgcaat gccgaagaag gggtgcaaaa
     3961 tgcgtcccat ctgcggcgta ccgaaggttt gcgtcagcca ttgcagcgtc gggaagaaga
     4021 acgaaatccc ggacagcgcc accaggaaga agcaaatcac cacggtccag tgacaggcgc
     4081 gatcaataaa tttggtgcgc acaatcattt tcgacttact catgatgatc ctcctcgtca
     4141 tcgtccactt ccttattcgg gccaataccg atgtagtgga aaatcaaccc ggcaaaagtg
     4201 gcaataaagc cagccgctgc cagcggtttc aacgcgcctt tccacaggct taccgaggtg
     4261 tcgatcttcg gatctttcgg cagaccgtga tacagctccg gctgatcggc gtgatgcagc
     4321 acgtacataa cgtgcgtacc accgacccct tccgggttgt agacgccagc atgttcgtaa
     4381 ccacgcgctt tcagtttcgc cacgcgctgt tccgccagct ccagcatctc cttcttggtg
     4441 ccgaagtgga tagccccggt cggacaggtt ttcacacaag ccggttcctg gccgacgctg
     4501 acgcgatcga cgcagagcgt gcatttatat acccggttat cctctttgtt gaggcgcgga
     4561 atattaaacg gacacccggc aatgcagtaa ccacagccga tgcagttttc cgactggaaa
     4621 tcgacaatcc cgttagcgta ctgaatgatt gcaccagcag acgggcacgc cttcaggcag
     4681 cccggatctt cacagtgcat acagccgtct ttacggatca gccactccag cttgccattc
     4741 tgttcggttt cgctaaagcg catcaccgtc caggacttgg cgctcagatc ggcggggtta
     4801 tcgtaaaccc cgacgcagtg ccccacttca tcacggatgt cgttccactc cgaacacgcc
     4861 acctgacagg ctttacagcc gatacaggtg gaaacgtcga taagtttcgc gacttctgct
     4921 ttgtaatcac gcacctgaga aggcggcgtg atggagttag ttgcggacct tttgataata
     4981 tcctgcgttt ccatagccat ctgttcgccc ccttacgcct tctcgatgtt gactaagaac
     5041 gctttatatt ccggcgtttg cgagtttgca tcaccgacat tcggcgtcag agtgttagcg
     5101 atataacctt tacgcgcgac accctcaaag ccccagtgga ttggaatacc caccgtttca
     5161 acctgctgac cgtttacatt cagcggtttc agacggcgcg ttaccactgc caccgcgcgg
     5221 ataaagcctc gcttgctgga gacagtgaca cgatcgccat tattaatgcc tttcgccgcc
     5281 gcctgcgttt cgctgatttc cacaaactgt tccggctgag caattgcgtt gagcaacgcg
     5341 tgcttggtcc aggtgtggaa gtgctcggtc agacgatagg tcgtacccac atacgggaac
     5401 tgctcttttt tacccatccg cagcgcgtct tgttcataca gacgaacaac cgggttagac
     5461 accacgttcg gatgcagcgg gttagtgccc agcggcgttt caatcggctc gtagtgttcc
     5521 gggaacggac cttccgccat tttgttgatg gcaaacaggc gtcccatccc ttccggctgc
     5581 ataataaacg gcccggttgg cgtacccggt gcggcattgc cgaagtccgg aatatcgtta
     5641 cccgtccact tgctgccgtt ccactggatc agcatccgtt tcggatccca cggtttaccg
     5701 ttgatatccg ccgaagcacg gttgtagagt acgcgacggt tgagcggcca cgcccaggcc
     5761 catcccagcg tattccccag accggacggg tcggagttat cgcggtttgc catctggtta
     5821 ccctgctctg tccagctacc ggtgtagatc cagcaagaag atgcggttgt accgtcatca
     5881 cgcagatgcg caaagctact cagcaactga cctttcttcg caatcagcac gccattagca
     5941 tcatagagat cttccaacgc atagccgttg ttctctttag ctacttcgtc agattgcggt
     6001 tcgtgcggct gcttgtagtt ccagctcatc ttcatcagcg gttctacgcc tttaccacct
     6061 tcggcctggt acaactcgcg cagatgatgg tagatacccg ccagaatttc gccgtcgtta
     6121 cgcgcttcgc ccggcgcgtc ctgacctttc cagtgccact gcaaccagcg accggagtta
     6181 gcgatagaac catcctcttc agcaaagcag gtcgaaggca gacggaatac ttcagtctga
     6241 atagacgccg gatcgacatc gttcgactcg ccgtggttct gccagaaggt agaggtttca
     6301 gtcaccagcg gatcgataac caccatgtac ttcagtttgc tcaggcagct caccactttg
     6361 tttttatccg ggaaggacgc aaccgggtta aagccctagc agaaataacc ggtaactttg
     6421 ccttcatcca tcatgttgaa atacttgatg acgtcgtagg tctggtccca cttcggcagc
     6481 cagtcatagc cccagttgtt ctctttctgc gcggcatcgc catagaaaga tttcatcagg
     6541 ctaacgaaga acttcggata gttgctccag tagttcacct gatcagccag cgtcgctttc
     6601 ggcgtgttcg cttccagata cgactgcaaa tcaacctgtt tttctgacgg cagcgtcaga
     6661 taacctggca ggctggtaga gagcagacct aagtcagtca gcccctgaat gttggagtga
     6721 ccacgcaatg cgttcacgcc gccaccggcc atacccatgt taccgagcag cgactggatc
     6781 atcgccatag tacggatgtt ctgcgcaccc acggtgtgct gcgtccagcc cagcgcgtac
     6841 aggaaggtgg ttgtgcgatc cggtgcgctg gtggaggcca gcacttcaca cactttcagg
     6901 aagtcggctt ttggcgtacc acagatgttt tcaacaacgt ccggcgtgta gcgggaaacg
     6961 tgcgctttca gcaggttcca cacacagcgc ggatgagtca gtgtttcatc gcgtttcgca
     7021 tagccgtttt catcgaactg atagttccag gacgatttgt cgtactggcg tttttcagcg
     7081 tcataaccgc tgaacaggcc atcttcgaaa gcaaaatcat cacgcaccag taggctggcg
     7141 ttggtgtaat gtttaacgta ttcggcgttg attttgttgt tttcgatcag gtagcgcaaa
     7201 atgccagaca ggaacgtaat gtccgtaccg gaacgaatag gtgcgtaaat atccgccaca
     7261 gaagcggtac gcgtaaaacg gggatcgaca acgatcaagg ttgcatcgtt gttgtttttc
     7321 gcttccatcg cccagcggaa accgacggga tgcgcttcag cagcgttacc gcccattacc
     7381 attacgacgt tagcgttttt gatatccacc cagtggttgg tcatagcacc gcgaccaaat
     7441 gttggagcaa gacttgctac cgttggtccg tgtcagacgc gcgcctggtt gtctaccgcc
     7501 agcatcccga gggagcgggc aaatttctgg gtcagcatcc cggtttcgtt gctggcaccg
     7561 gaggcacaca gcataccggt agaaagccaa cggtttaccg ttacgccctg ctcgttcttt
     7621 tcaataaagt tagcgtcacg gtcagctttc atcagcttcg caatacggga gaatgcttct
     7681 tcccagctaa tgcgctgcca tttgtcagaa cctggcgcac gatatttcgg gtagcgcaga
     7741 cggttttcac tgttgacgta atccagcaaa ccggcccctt tcgggcacag cgcaccacgg
     7801 cttaccggat gatccgggtc accttcaatg tgataaatcg cttctctggc gttttttgcg
     7861 ccatcaccca ggctatacat caatagcccg caacctacgg aacagtatgt gcaggtgtta
     7921 cggatctctt tagcgcgtaa taatttgtag tttcgcgcct gagccagtgc ttgcttcggg
     7981 gcaaagccca atgccgctac cgttgttcca gccataccgc ccgcgcagat tttaaaaaat
     8041 tgtctgcgac tgacgtccat tgctttcctc ttttttcagg ggtattactg cgcgaggaaa
     8101 ctaacagtaa aattctgaat ctttttgcgc gaaatcaaaa agaacacgtg tttgccacaa
     8161 aataatccgc ctgcgcaaat tgacctacct caatagcggt agaaaaacgc accactgcct
     8221 gacaggccag ttaaaaaaat gctataaaat tcagcttaat ttttaacggc aagagagaca
     8281 aaacagcgag catgacacga caaaaagcaa cgctcatagg gctaatagcg atcgtcctgt
     8341 ggagcacgat ggtaggattg attcgcggtg tcagcgaggg gctcggcccg gtcggcggcg
     8401 cagctgctat ctattcatta agcgggctgc tgttaatctt cacggttgga tttccgcgta
     8461 ttcggcagat tccgaaaggc tatttactcg ccgggagtct gttattcgtc agctatgaaa
     8521 tctatctggc gctttcctta gggtatgcgg cgacccgtca tcaggcgatt gaagtgggta
     8581 tggtgaacta tctgtggccc agcctgacaa ttctctttgc cattctgttt aatggtcaga
     8641 aaaccaactg gctgattgta ccaggattat tattagccct ggtcggcgtc tgctgggtgt
     8701 taggcggtga gaatgggtta cattatgatg aaatcatcaa taatatcacc accagcccat
     8761 tgagttattt cctggcgttt attggtgcgt ttatctgggc agcctattgc acagtaacga
     8821 ataaatacgc acgcggattt aacggaatta ccgtttttgt cctgctaacg ggagcatgtc
     8881 tgtggattta ctattttctc acgccacaac cagaaatggt atttagcacg cccgtcatga
     8941 ttaaactcat ctctgcggca tttaccttag gatttgctta tactgcatgg aatgtcggta
     9001 tattgcatgg caatgtcacc ataatggcgg taggctcgta ttttacgcct gtactttcct
     9061 cagcgcttgc agccgtgctg ctcagcgccc cgctgtcgtt ctcgttctgg caaggcgcac
     9121 taatggtctg cggcggttcc ctgctctgct ggctggcgac acgtcgtggt taaaataatt
     9181 gccgggtcta cggcccggca gttaatcaga taaattccga caatattaaa aaataaattt
     9241 caaataccag taattcacct tatatctcat ttcgacttcg atcacatatc gataaataat
     9301 attattctat ttttctcatt gtttttaatc accattctat tttaaaaaca gtatgtcatt
     9361 tataaatgac gccgtttaaa attcgtttag aaaatacgtt taacgataat taaaaggatt
     9421 ctgttaaatg aaattaaaaa tagttgcggt ggttgtaact ggtttgttag ctgcgaacgt
     9481 agcacacgct gccgaagtct ataacaagga tggtaataaa ctcgaccttt atggcaaggt
     9541 taccgctcta cgttatttta ctgatgataa gcgtgacgat ggtgataaaa cttatgcccg
     9601 tctcggcttt aaaggagaaa cgcaaatcaa tgatcaaatg attggttttg gtcactggga
     9661 atatgatttt aaaggctata acgatgaagc caacggctcg cgcggcaaca agacccgtct
     9721 ggcctatgct ggtttaaaaa ttagtgaatt tggctctctg gactatggtc gtaactacgg
     9781 tgtcggctac gacattggtt catggaccga tatgttgcca gaatttggtg gcgatacctg
     9841 gagtcagaaa gatgtcttca tgacataccg taccaccggt ctagcaacct atcgcaacta
     9901 cgatttcttt ggcttaattg aagggctgaa ctttgccgcg caatatcaag gcaaaaataa
     9961 acgtactgac aacagtcatc tttatggtgc tgacgacacg cgtgccaacg gtgacggttt
    10021 cggtatctcc tcaacttatg tttatgatgg ctttggtatc ggtgcggtat ataccaaatc
    10081 cgatcggaca aatgcgcagg aaagagccgc agctaatcct ctcaatgcct ccggtaagaa
    10141 tgcagaacta tgggccacag gtataaagta tgatgccaac aacatctact ttgcagctaa
    10201 ttacgatgaa acattaaaca tgaccaccta tggggatggt tatatctcta acaaagcaca
    10261 aagttttgaa gtggtggcgc aatatcaatt cgacttcggc ttgcgcccat cactcgctta
    10321 cctgaaatcg aaaggcagag atctgggccg ctacgccgac caggacatga ttgagtatat
    10381 cgacgttggt gcgacgtatt tcttcaacaa aaatatgtcg acctatgttg attataaaat
    10441 caacctgatt gatgaaagcg actttacccg tgccgtagat attcgcaccg ataacatcgt
    10501 cgctacgggc attacctatc agttctaacg tcctctcctg accagccctt ctctgggctg
    10561 gtttttgatt taagtgctgc tgtatgactc cttaagtaaa aatgagcaat tcttgatatt
    10621 tctggtcatt cacctcaatt aatattattc catatcctcc ggggacatct tactataata
    10681 tttattgatg agtgattatg accaatatta atacagcttg tgtaaaaaat aatgccagtt
    10741 atcaacttaa taacgcatta cctaacaaag aaaccatctc cagcaatttt tgtgaacgac
    10801 tagaacaatg gggtaataaa tcggttaata atggtgaaga aagagcaatt gccgtggagc
    10861 gaattaaaga agcttacaat tcgaacatgg catctcttga tttgtcatat cttgatttga
    10921 gtgaattacc gcctatcccc tcgacagtta atacgttaaa tctggaaaac aattgtctaa
    10981 cttgtcttga cttagctgac aatgccagtc tcgttaatat caacctcagc gttaataaaa
    11041 ttaacacgat aacctttcca aaagaaccaa aactggaaaa catttatatt aatcataaca
    11101 atctggaaag tttagattta aaaaatcagc attcattggt taatctggaa gcgcaaaata
    11161 atagcctaaa aaaatgatat ttctgatagt tataaactga aatttcttaa tcttgattat
    11221 aacaagctag catcgctgga tgtctcccgg caagaatctc tgattgagtt aagtgcccat
    11281 cacaatatga tcactgacct tatattacac aatcatccca ggatgaaaac aatcacttta
    11341 aacgacaacc atattgcaca tttaaacgcc aaaaacacta caaaactgga atatttaaac
    11401 ttaagcaata acaatttact gccaaccaat gacattgatc aactaatatc atcaaaacat
    11461 ctttggcatg tattagttaa cggcatcaac aatgatccac ttgcacaaat gcagtactgg
    11521 actgcagtaa gaaatataat tgatgacact aatgaagtga ccattgattt atcaggactt
    11581 aatttaacca ctcaaccacc agggctgcaa aacttcacct ctatcaatct tgataataac
    11641 caactcacac attttgatgc aaccaactac gatagactcg taaagctaag tctgaatagt
    11701 aatgctcttg agtcaataaa ttttcctcaa ggcagaaatg taagtattac acatatatct
    11761 atgaataata atgctctcag aaatattgat atagataggc tttcatcagt tacttatttt
    11821 agtgcggcac ataatcaact agagtttgtg caattagaat cttgcgaatg gctgcaatac
    11881 ctgaatctca gccataatca attaactgat attgttgcag gtaataaaaa tgaactctta
    11941 ctgctggatc tatcccataa taaactaaca agtttacaca atgtcttatt tcccaacttg
    12001 aatacgttac ttattaacaa caatttgctt tctgaaatta aaatattcta tagcaacttc
    12061 cgcaatgttc agacattaaa cgctgctaac aaccagttga aatatataaa tcttgatttc
    12121 ctgacttatc ttccatctat caaaagttta agactggaca ataataaaat aacccacatt
    12181 gatactaata atacatccga tattggaact ttattcccca taataaaaca gagcaaaaac
    12241 ttaaattttt taaatgtttc tgggaagaac aattgaccta ctatgcagct catgttattt
    12301 aatttgtttt ccccagcact taagcttaat actggcctgg caattctttc gcctggtgca
    12361 tttgaagttc actctgacgg aatagatgcg gataacgaat tgtttcacta tactattaaa
    12421 aaagcatata ccccatataa tatacacact tacaagacag aggaagttgt aaaccagagg
    12481 aatataaa
//
LOCUS       DAJTQQ010000104        12170 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000104 DAJTQQ010000000
VERSION     DAJTQQ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12170
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.104"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            506..589
                     /locus_tag="OO309_003311"
     CDS             506..589
                     /locus_tag="OO309_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR5848340.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(643..1995)
                     /gene="gorA"
                     /locus_tag="OO309_003312"
     CDS             complement(643..1995)
                     /gene="gorA"
                     /locus_tag="OO309_003312"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR5848341.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2067..2909)
                     /gene="rlmJ"
                     /locus_tag="OO309_003313"
     CDS             complement(2067..2909)
                     /gene="rlmJ"
                     /locus_tag="OO309_003313"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR5848342.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3112..5154
                     /gene="prlC"
                     /locus_tag="OO309_003314"
     CDS             3112..5154
                     /gene="prlC"
                     /locus_tag="OO309_003314"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR5848343.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5162..5914
                     /gene="rsmJ"
                     /locus_tag="OO309_003315"
     CDS             5162..5914
                     /gene="rsmJ"
                     /locus_tag="OO309_003315"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR5848344.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(5963..7432)
                     /gene="dtpB"
                     /locus_tag="OO309_003316"
                     /pseudo
     CDS             complement(5963..7432)
                     /gene="dtpB"
                     /locus_tag="OO309_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(7750..8184)
                     /gene="uspA"
                     /locus_tag="OO309_003317"
     CDS             complement(7750..8184)
                     /gene="uspA"
                     /locus_tag="OO309_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR5848345.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8578..8913
                     /gene="uspB"
                     /locus_tag="OO309_003318"
     CDS             8578..8913
                     /gene="uspB"
                     /locus_tag="OO309_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR5848346.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(8984..10483)
                     /gene="pitA"
                     /locus_tag="OO309_003319"
     CDS             complement(8984..10483)
                     /gene="pitA"
                     /locus_tag="OO309_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR5848347.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10715..11914
                     /locus_tag="OO309_003320"
     CDS             10715..11914
                     /locus_tag="OO309_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR5848348.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         2990 a         3097 c         3249 g         2834 t
ORIGIN      
        1 tcaatactat tagccagtgg ctaacattgc aagcgttagc gatttcttca gctggagaag
       61 ggacagtttg ctatacatgg tggtgaaaga ggatttatat actttcatcg ggtcgctgcc
      121 gtaccaagcc ttgtccgaat ccggacgtaa aaaaaccgac ttcgcgtcgg tttttttact
      181 cttcagccca gagttggtgg ctctggctgg agtgagaatg ccatcatcta acgcatcaat
      241 gctaatacca tactgaaact attgcaaggt tatgctggtt atttagccta attacactgt
      301 atgattatcc agttagctct gaggcatttt cactctggca atgcgcataa acgctttcaa
      361 agtcctggtc agaagtacgg gtggtgccgt taactgatgc tctggccgga gtgagattgc
      421 ccacatctaa caatgaggca tgcgccgtga caggcagtgg atgagtaagc ggatgcattc
      481 tcactccatc gcatggagaa aacgggtgat tgataaagca atcatcgttc ttggggcgtt
      541 aattgcgctg ctggaactga tccgctttct gcttcagctt ctgaactgat agcggaaacg
      601 taattaaggg ctaagagcac actactctta gccctttaac atttaacgca tggtcacgaa
      661 ctcttctgcc gccgttgggt gaatggcgac ggtattgtcg aagtcttttt tggttgcgcc
      721 catcttcagc gccaccgcga agccctgcaa catttcgtcc ataccaaagc caatgccgtg
      781 aataccgaca atcttctctt ctggcccaac gcacaccagc ttcatgcggc acggctggcg
      841 gtgagtggtg acggcggtat acatcgcggt gaaagaggat ttatacactt tcacctgatc
      901 gtcgccgtac tgctcgcgcg cctgcggttc cgttaaacca acagtaccaa tcggcggatg
      961 gctgaagacc acggtcggaa tgttgctgta atccagatgc tcatccggct tgttattaaa
     1021 caggcgttca gagagacggc gacccgctgc aactgccacc ggtgtcagtt ccactgcacc
     1081 cgtgttatcg cccaccgcgt aaataccttc aatattggtg ttttgatatt tatcgacgac
     1141 gatatagcct ttttcgttgg ttttaacgcc agcggcttcg aggttgatgt tgtcattggc
     1201 aggctcgcga ccaatcgccc aaatcaggca atccaccgtt tcactgcgac catcttccag
     1261 ctccagcgtc aggctaccat cggcattttt cactaccgct ttcgggatgg cgttggtatg
     1321 caactgtggg ccttcggcgt tcatcacttc gaccagcgtt tcagagatca tcgggtcgaa
     1381 gctgcgcagc ggcgcatgtt tacgcacaaa cagatgcgtt ttcgcgccga ggccgttaat
     1441 cacgccagcc agctcaacgg cgatgtaacc cgcgccaaca accgccacgc gctctggcaa
     1501 agcaggaagg gcgaagaagc catcggaatc aataccgtat tccacgcctg gaatatccgg
     1561 gtggctcgga cgaccgccgg tggcgatcag aatatgatcg gccgtgatgg tttcgccgtt
     1621 aacttccagc gttttggcat caacgaagcg ggcaaagcct ttgattacat caacgttatt
     1681 tttaccgagc acgttttcat aggaagtatg aatacggtcg atataggcgg tacggctggc
     1741 gatcaacgtt tcccagttga acttgttgat ggtggtatcg aaaccataat cagggccgta
     1801 catatggatc gcttcacgga tttgcgccgc gtgccacatc acttttttcg gcacgcagcc
     1861 gacgttcacg caggtgccgc ccagctcttt ggcttcaatc agtgcgcatt tctggccgta
     1921 catagccgcg cggttgatgg aggcgatacc gccgctgccg ccgccgatgg cgatgtaatc
     1981 atagtgttta gtcatgacat attgtcctta tcgttgatta ccgcgattgt agcgcgagac
     2041 gtaataggtg ccagcaatgg ctgcaattac tccggcacga tccagcttac ggtggcgtgc
     2101 ccggtacctg ccggaaccag tttgctgtgc agccacgaca gcacgttatt catctgctgt
     2161 tccagcttcc acggcgggtt aatcacaatc atgccggaag cggtcatgcc acggcgatcg
     2221 ctgtctggca gcaccgccag ttcaatttgc agaattttgc gaatgccggt cgcttccagg
     2281 tcgtggatca tgcgcttaat ttgctggcgc agcaccaccg gataccacaa tgcgtaagtg
     2341 ccagtggcga aacgtttgta accttctgct atcccgctga ccaccgcctg atagtcggtt
     2401 ttcatttcat acggcgggtc gataaggatt aaaccacggc gagaaaccgg cggcagtttg
     2461 gccttaagtt gctggaaacc gtcggctttt tcgacgcgtg cacggctgtc tttctgaaat
     2521 ccagaacgca gcaacgggta atcgctcggg tgcagttcgg tcagttgcag gctgtcttgt
     2581 tcgcgcagta gctggcgagc aatcaacggc gaaccggggt agtaacgcaa ctggccgcta
     2641 cggttgaagt gttttaccac attgatgtac gcctccagtt ctgcgggcaa atcgtcctgc
     2701 tgccagatac gggcgatacc ttcgagatat tcgccggtac gctcggcatg ttcgctacct
     2761 aactgataac gcccggcccc tgcgtgggtg tcgagataga gaaacggttt atctttctct
     2821 ttcagcgact cgatgatcag gctctgaacg gtatgtttaa ggacgtcggc gtggttgcca
     2881 gcgtgaaagc tgtggcgata actgagcatg ggtaaaggtg ttccggtaag taaaaattgg
     2941 cccgtattcg gggcgcacaa aagcctatca ggacagtata ccgaaaagag gccgccgccg
     3001 cgaaagcgta acgtttctca ttgaaattca ctacacttaa ccccatgcta cacacattat
     3061 gtaaagcgcc tgttgagcgc ttccttaacc tctttaacca ggactgcgct aatgacgaat
     3121 ccgttactga ctccctttga attgcctccg ttttctaaaa ttctcccgga acatgtcgtt
     3181 ccagccgtga ctaaggcatt gaacgactgc cgcgaaaatg tggagcgcgt agtagcgcaa
     3241 ggggcaccgt acacctggga aaatctctgc cagccgttgg cggaagtgga cgatgttttg
     3301 gggcgtatct tctccccggt cagccacctg aactcggtga aaaatagccc ggaactgcgt
     3361 gaagcgtacg aacaaaccct gccgctgctg tcggaataca gcacctgggt agggcaacat
     3421 gaagggctgt ataaagcgta ccgcgacctg cgcgatggcg atcattacgc cacgctgaac
     3481 acggcgcaga agaaagcggt tgataacgcc ctgcgtgatt ttgaactctc tggcataggt
     3541 ctgccgaaag agaaacagca gcgttacggc gaaattgcta cccgtctttc tgaactgggc
     3601 aaccagtaca gcaacaacgt cctcgatgcg acgatgggct ggaccaaact cgttaccgac
     3661 gaagcggggc tggcggggat gccagaaagc gcgctggctg cggcaaaagc ccaggccgaa
     3721 gcgaaagagc tggaaggcta cctgctgacg ctggatatcc caagctacct gccggtaatg
     3781 acctactgcg acaaccaggc tctgcgcgaa gagatgtatc gcgcttacag cacccgcgcc
     3841 tccgatcaag gcccgaatgc cggtaagtgg gataacagca aggtgatgga agagatcctc
     3901 gcgctgcgtc acgaactggc gcaactgctg ggctttgaaa actacgcctt taaatccctt
     3961 gccactaaaa tggcagaaaa cccgcagcag gtgctggatt tcttaaccga tctggcaaaa
     4021 cgcgcgcgtc cgcaaggcga aaaagagctg gagcaactgc gtgccttcgc taaagccgaa
     4081 tttggtgtcg atgagttgca gccgtgggat atcgcttact acagcgaaaa acagaaacag
     4141 cacctctaca gcatcagcga cgagcagctg cgtccgtact tcccggaaaa caaagcggtt
     4201 aacggcctgt ttgaagtggt gaaacgtatt tacggcatca ccgctaaaga gcgtaaagat
     4261 gttgatgtct ggcatccgga tgtacgtttc ttcgaactgt atgacgaaaa taacgaactg
     4321 cgcggcagct tctacctcga tctgtatgcc cgtgaaaaca agcgcggcgg ggcgtggatg
     4381 gatggctgcg taggccagat gcgtaaagct gatggttctc tgcaaaaacc ggtcgcgtat
     4441 ttgacttgta acttcaaccg cccggtaaat ggtaaaccgg cgctgttcac tcacgacgaa
     4501 gtgatcaccc tgttccacga gttcggtcac ggcctgcacc atatgctgac ccgcatcgaa
     4561 accgctggtg tttccggtat cagcggtgtg ccgtgggatg cggtcgaact gccgagtcag
     4621 tttatggaaa actggtgctg ggagccggag gcgctggcgt ttatctctgg tcactatgaa
     4681 accggcgaac cgctgccgaa agagttgctg gataaaatgc tggcggcgaa gaactaccag
     4741 gcggcgctgt ttattctgcg tcagctggag ttcggcctgt ttgatttccg ccttcatgcc
     4801 gagttccgcc cggatcaggg ggcaaaaatc ctcgaaactc tggcagaaat caagaaactg
     4861 gttgccgtgg tgccatctcc gtcctggggc cgtttcccgc acgctttcag ccatattttc
     4921 gccggtggtt atgcggcagg ttactacagc tacctgtggg ctgacgtact ggcggcagat
     4981 gctttctcgc gctttgagga agagggcatt ttcaaccgtg aaaccgggca gtcgttcctc
     5041 gacaacattc tgagccgtgg cggttcagaa gagccgatgg atctgttcaa acgcttccgt
     5101 ggtcgtgaac cgcagctgga tgcgatgctg gagcattacg gcattaaggg ctgatcattc
     5161 agtgaaaatc tgcttaattg atgaaacagg cgccggagac ggcgccttat ctgttctggc
     5221 ggcccgctgg gggctggagc acgatgaaga caacctgatg gcgctggtgt taacgccgga
     5281 acatctggaa ttgcgcaagc gtgatgagcc aaaacttggc ggcatctttg ttgattttgt
     5341 tggcggagcg atggcgcacc gacgcaaatt cggcggtggt cgcggtgagg cggtggcgaa
     5401 agcggtgggc attaaaggcg attatttgcc ggatgtggtg gacgccaccg cagggctggg
     5461 gcgcgatgcc tttgtactgg cttcagtcgg ctgccgcgtg cggatgctgg agcgtaatcc
     5521 agtggttgcc gcgctactcg acgacggcct ggcgcgtggt tatgcggatg cggaaatcgg
     5581 cggctggttg caggagcggt tgcagttaat tcacgcctcc agcctgacgg cgctaaccga
     5641 tattacccca cgcccgcagg tggtctatct cgatccgatg ttcccgcata agcagaaaag
     5701 cgcgctggtg aaaaaagaga tgcgtgtctt tcagtcgctg gtggggccgg atcttgatgc
     5761 cgatggatta ctggagcctg cacgcttgct ggcgaccaaa cgcgtggtgg taaagcgccc
     5821 ggactacgcg ccaccgctgg cgaatgtcgc cacgccaaac gcggttgtga ctaaagggca
     5881 tcgctttgat atctatgcag gtacgccagt gtaaaagtaa agcagggtag cgataacgct
     5941 accctgcttt ctgcaagaat aattaatggc tttccggcgt cgcaatcatg cgtttcagcc
     6001 acggcaccat caacagcatc actactgcaa cgcccagcgt gaccagacca atcttaccaa
     6061 acacgttggt atagacgggc aacgtctcaa gcggatcggt aatgttgtcc ggcaccgcgg
     6121 taaatgttgc cacatagccg cccagcaaga acgcggcagc ctgcgtcagg aaccacatcc
     6181 cgagaataaa gcccatcaaa tgctgcggca ccagggcagc aatcatcgcc aggccaaggg
     6241 cgctaataaa caattcaccc aggctctaga ataaatacac cagcacgata aaccatggcg
     6301 atgtcagccc ctggtcatcc gcaaaccaca ttcccgcagc agcagccgtc aaaaagccca
     6361 gtgagcacat aaacatgctg agagtaaatt tcatcggcat cgagaggtct ttgcctttgt
     6421 tacccagatg cgtgtaaatg cctgccagta ttgggctggc gagtaccacc cagaacgggt
     6481 taagcgcctg gaagctgacc gggttgatgg aaaaaccgag aatttcatga tgcacgttgt
     6541 tgatggcaaa gaagttcagc gatgttggca tctgggcgta gagaatgtaa aacaccaccg
     6601 cttcgagcat caggacaaag gcgacaaaca ttttattgcg cccggtttta tccagcttga
     6661 atgcctgacg aaagaagatg atagtgacga cgatggagag aacaatcagc accagattgg
     6721 cgacttctac gttgtgcatc agccatgcac atacgaagat catcaccacg ctgccaagta
     6781 atacgtacaa cagtttgctg aagctcatcg gcttgaagtc gggttcagaa ccaatgtctt
     6841 tcaccattcc acgacaggcg atgtaaacca gtaatgcgat aattaacccc gccccgcaca
     6901 ggttgtaggt gactgaataa ccgaatctat cagcgatcac aggggccagc gataacgcta
     6961 tcaacgagcc gatattgatc gacatataga acagggtgaa tgcgccatca agccgcggat
     7021 ctttcggcgg atagcacttc gaaagcaagc tggctgggtt agctttaaac aggccgttac
     7081 cgacagcgat agtccccagg gcgatgaaaa tcaggtcagg cttaagtagc gacatgccgg
     7141 tcatgaagta gccaatcgcc agcacaagtg ctccaagaac aatggtgcgt ttggtcccca
     7201 acaggtggtc gccgacatag ccgccaatgg aaatgaggcc atagaccagt gcagcaaaag
     7261 caccaaaagt gacaaaagcc tgctcttgcg agaatccaag ctgtttaacg aagaaaaccg
     7321 ccagtacgcc ctgcacgccg tagtagccga atcgctccca taactcgaca aaaaagatca
     7381 tgaaaaatgg gcgaggttgc tgcagcatcc ccatgggtgt tgttgtatta atatttatta
     7441 accttccaat accatcctga aaagtatgaa cgcgtaaggg ataacgcgcg aaaaagttta
     7501 catctgtcag agattcttaa tattgatgcg gtgaatagat taatacacta ttacaacaga
     7561 aaataaccag ctgatcacac taatgatgga aaatatgatg gtgttaaatt ttgtattggc
     7621 aggagatttt gattgcgtac gcagatcgta ggccagataa ggggtttacg ctgcatcagg
     7681 taacaaggcg cgccaaaaag caaaacgccc gcatatgcgg gcgttcagga acacgtcgtg
     7741 gagggatgat tattcttctt cgtcgcgtag cggaacaatc agcatatcaa cgtgaacggt
     7801 gttgatcagc tgacgtgcgg aagacatcag tttgctccag aagtcctggt ggtgaccaca
     7861 aaccaccaga tccatatcgt atttcttgat tgcatcgacc agaacctggc ctaggtcgcc
     7921 gctgccgctc agggtttcag tgattggata gcctgcatta gtggaaagct cggtcagcgc
     7981 atgatgtgtc tcttcagaga tgcgtttctg catatcaccc agattcacat caataagccc
     8041 ggtgtatagg tcagagtagt ttacatctac gtggatcaga gaaactttcg cattgtaggg
     8101 gcgagccata gagactgctt tctctaccag aactttgctt tccggggaga gatcgactgc
     8161 gatgagaatg tgtttataag ccatagtgtt actccttcca taaagttgtc gatgactggc
     8221 cagctagcgt ttcttgtgcc gttacgtcac ccgcgtcctg cgaactacgt gcgcggggct
     8281 aacccagccg taaagattca gtgttaagac catccttacc ttatagcgac ccggatgatc
     8341 cgtcaatccg ccttgcttac ccgttagata aacaaatttt cgacatgaat attatattga
     8401 tagtggttaa ccttctggaa aaaaaacaac ctgatctcct acactatcta tagagccgct
     8461 cggatgttgg tcaccacaaa agcggtttct ccaggacctg gtgttaactg attggcagcg
     8521 tatcgggagc ggtagccccg gggtggaact ccgcgggcag gtcgccgggg aggaggtatg
     8581 ataagcaccg tcgcattatt ttgggcttta tgtgtcgttt gcattgttaa catggcgcgc
     8641 tatttctcat cactacgcgc gttgttagtg gtactgcgta actgcgatcc attgctctat
     8701 caatatgttg atggaggggg cttttttacc tcacatggcc aacccaacaa acaggtgcgt
     8761 ctcgtttggt atatctatgc ccaacgttat cgcgatcatc acgatgatga gtttattcgc
     8821 cgctgtgagc gggtgcgtcg gcagtttatt ctgactagcg cattgtgtgg tctggtggtg
     8881 gtcagcctga ttgcattgat gatttggcat taagcacttc agcaataata acgtataaaa
     8941 aaagcgggcc agtaaactga cccgctcgtt ttggtgcgta cgattacagg aactgcaagg
     9001 agaaccagta cagcccgccg gaaagtagta ccgcagccgg aagggtaaac acccaggcca
     9061 tcagaatact ggttacggtt ttacgctgta agccgccgcc atctaccacc atcgtccccg
     9121 cgacagaaga ggagagtacg tgagtggtgg aaaccggcat cccggtataa ctcgccaggc
     9181 cgattgacac tgccgccgtc atctgggcag acatcccctg agcgtaggtc atgcctttct
     9241 taccgatttt ctcaccgata gtcgttgcca cacgacgcca gccaatcatc gtaccgatac
     9301 ctaacgccag cgcgaccgcc atgatgatcc acaccggcgc atactcgatg gtgctaagca
     9361 tgtcggactt cagtttcttc aacaggcgct gatcgtcagc actcacacca ggcatcttca
     9421 ccactttgtc gatagtgtca gaaacgcaca gcataatgcg gcgcatctgg ctacgttgat
     9481 caagcgacag cttgtcgtag ctttccacat cggtggtcaa catacctttc aggcggttga
     9541 gcgcgttaat ggtattcgac ggatggcagt ggaactccgc aggttgcgtt gcgccagctt
     9601 ccggagccgg tactaactga tcagcaccgg tagcctgttt gagcagcgca ggatgctgct
     9661 caaagtaagc ttcgacgttg ttgatggcat cacgggtacg ggtgatttcg tagccagtgg
     9721 cattcatgtt caccacgaag cctgctggcg cgacgccaat caataccaac ataaccagac
     9781 caatgccttt ctgaccatcg ttcgcgccgt gcgaaaacgc cacgccgata gcggaaagga
     9841 tcagcgcgat acgcgtccag aacggcggct ttttcttgcc gtctttcttt tcacgctccg
     9901 ctggggtcag gtggatacgg gcgcgtttct tggtgccgct ccagtagcga cgcagcaaga
     9961 aaatcagacc gccagcaaac accaggccga caatagggga aacgatcaga gaaccgaaaa
    10021 tacttaatac tttcgggata ttgagtgcat ccaccactga cgtcccggtc atcaacgcat
    10081 tggttaaacc aatcccgatg atcgcgccaa tcagcgtatg agagctggat gcaggtaaac
    10141 caaagtacca ggtacccagg ttccagataa tcgccgccag caacatagag aacaccatgg
    10201 caaggccatg agacgatccc atattaagca gcagatccgt cggcagcata tgcacaatgg
    10261 cataggcaac actcagacca cccagcaaaa cacccaaaaa gttgaacacc gccgccataa
    10321 ccacggcgag ctgagaacgc atcgcgcggg tatagataac ggttgccacg gcgttggctg
    10381 tgtcatggaa accattgatg gcttcgtaga acagcacaaa agccagtgca agcaataata
    10441 acagcccggt atgcaaatcc aggccagcaa acaaatgtag cataggacgt tacgccattt
    10501 tgaggacatg aacgcggcgc attatcagtg actttcgcgg cgcgggcaaa gtgaaatata
    10561 gacttttttt gatttgcctc ctgtatggat ttcactcaaa aaataattat cttatataat
    10621 tcaggcaaat acttcctttt agtaatattg atgctggtgc gaccactgag gaatctttac
    10681 aattcacgcc cgttttttct aagaggagcg caacgtggaa aggtttgatg ccattattat
    10741 aggcgctggt gcggcgggta tgttctgttc tgcgctggca ggtcaggtag gacgccgggt
    10801 tctgctgatc gataatggta aaatactagg gcgcaaaatc cttatgtctg gcggtgggcg
    10861 ctgcaacttt accaaccttt atgtcgaacc aggcgcttat ctgagccaga atccgcattt
    10921 ttgtaagtct gcactcgcac gttttaccca gtgggatttc attgatctgg tcaataaaca
    10981 cggtatcgcc tggcacgaga aaacgttagg gcaactcttc tgcgatgact ccgcgcagca
    11041 gattgtcgac atgctggtgg atgagtgcga gaagggcaat gtaaccttca gattgcgtag
    11101 cgaagtgctg agtgtggcga aggatgaaac aggcttcacg cttgatctga acggcatgac
    11161 tgtcggttgc gaaaagctgg tcatcgcgac cggtgggctg tcaatgccgg ggctgggcgc
    11221 atcgccgttt ggttataaga ttgccgaaca atttggcctc aacgtgctgc cgacccgcgc
    11281 gggtctggtg ccattcactc tgcataaacc gttgctcgaa gagttacagg tgctggcggg
    11341 cgtggcggtg ccttccgtga ttaccgctga aaacggcacc gttttccgtg agaacttact
    11401 cttcacccac cgcggcttat ctggaccggc ggtgttgcag atttcaagct actggcaacc
    11461 gggtgaattt gtcagtatca atctgctacc ggatgtggac ctcgaaacct tcctgaatga
    11521 gcagcgtaac gcacatccga atcaaagcct gaaaaacaca ctggcggttc atctaccgaa
    11581 gcggttggtt gaacgcttac agcaactcgg gcaaatcccg gatgtttcgc taaaacagct
    11641 caacgtgtgt gaccaacagg cactgattag cacattgacc gactggcgcg tacaacccaa
    11701 cggcactgaa ggctatcgca ctgccgaagt gacgctcggc ggcgtggaca ccaacgaact
    11761 ttcttcacgg acgatggaag cgcgcaaagt gcctgggctg tacttcatcg gtgaagtgat
    11821 ggacgttacc ggctggctgg ggggctataa cttccagtgg gcgtggtcga gtgcatgggc
    11881 ttgtgcgcag gatttggcgt caattgtgaa ataaccatga cgttttcccg tcagccgttt
    11941 gtatatgtcg ttattaagtc attttggtac ggttctgcat ccgacattct ggtgttaact
    12001 cgctgttttg ttagcgaatt aacgctattg ccgttgtggt tattttatag tgtatttatt
    12061 ctcagagaat atatattact gaaataaaaa acacaaagaa taaccaaagg aaattatttg
    12121 aaacgtaaaa ggtatatatt tttaatctgt aaaatttaaa atcacctcct
//
LOCUS       DAJTQQ010000105        12061 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000105 DAJTQQ010000000
VERSION     DAJTQQ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12061
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.105"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(4..380)
                     /gene="rnpB"
                     /locus_tag="OO309_003321"
     ncRNA           complement(4..380)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO309_003321"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(413..1558)
                     /gene="garK"
                     /locus_tag="OO309_003322"
     CDS             complement(413..1558)
                     /gene="garK"
                     /locus_tag="OO309_003322"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR5848349.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OO309_003323"
     CDS             complement(1655..2545)
                     /gene="garR"
                     /locus_tag="OO309_003323"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR5848350.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OO309_003324"
                     /pseudo
     CDS             complement(2575..3345)
                     /gene="garL"
                     /locus_tag="OO309_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OO309_003325"
     CDS             complement(3361..4695)
                     /gene="garP"
                     /locus_tag="OO309_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR5848351.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5070..6641
                     /gene="garD"
                     /locus_tag="OO309_003326"
     CDS             5070..6641
                     /gene="garD"
                     /locus_tag="OO309_003326"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR5848352.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="OO309_003327"
     CDS             complement(6693..7517)
                     /gene="agaR"
                     /locus_tag="OO309_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCR5848353.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7766..9046
                     /gene="kbaZ"
                     /locus_tag="OO309_003328"
     CDS             7766..9046
                     /gene="kbaZ"
                     /locus_tag="OO309_003328"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCR5848354.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            9069..9542
                     /gene="agaV"
                     /locus_tag="OO309_003329"
     CDS             9069..9542
                     /gene="agaV"
                     /locus_tag="OO309_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR5848355.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9553..10332
                     /locus_tag="OO309_003330"
     CDS             9553..10332
                     /locus_tag="OO309_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCR5848356.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            10322..11131
                     /locus_tag="OO309_003331"
     CDS             10322..11131
                     /locus_tag="OO309_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR5848357.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            11218..11652
                     /locus_tag="OO309_003332"
     CDS             11218..11652
                     /locus_tag="OO309_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5848358.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            11649..>12061
                     /locus_tag="OO309_003333"
     CDS             11649..>12061
                     /locus_tag="OO309_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5848359.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         2879 a         3252 c         3076 g         2854 t
ORIGIN      
        1 aacaggtgaa actgaccgat aagccgggtt ctgtcgtgga cagtcattca tctaggccag
       61 caatcgctca ctggctcaag cagcctaccc gggttcagta cgggccgtac cttatgaacc
      121 cctatttggc cttgctccgg gtggagttta ccgtgccacg gactgttacc agccgcgcgg
      181 tgcgctctta ccgcaccctt tcacccttac ctgatcccgc ttgcacgggc catcggcggt
      241 ttgctctctg ttgcactggt cgtgggtttc ccccccaggc gttacctggc accctgccct
      301 atggagcccg gactttcctc ccctccaccc gtctcccccg aagaggacga cgacgaagcg
      361 gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt tgcgcgccct tgtcaccccg
      421 tgttgcgcat tccaatcgcc agtgtcgcgg caatattacg tgaagcacgg cagatattgt
      481 cataagcccc gcggaatgct tcgtccaacg tacctatgct ggtcaatacg ctgaagaccg
      541 catcaatgcc atgctgatgt acaacgccaa catcatcggt caggctaccc gcaatgccaa
      601 tcaccggttt atggtacttc ttcgccacgt ttgcgacgcc aatcggtacc ttgccgtgaa
      661 tactctggct gtcaatacgc ccttcaccgg tgatcaccag cgtacaatcg tgaatatgtt
      721 cctccagatt cagcgccgta gtgacgattt caataccact tttcagttcc gcaccaagaa
      781 acgccatcag cgccgcaccc ataccgcccg cagctcctgc gccggggaca tcttttacat
      841 caacatgcag cgctttttta atgacatcgg catagtgaga gaggttattg tccagctcaa
      901 caatcatcgc ttcactggct cccttttgtg ggccaaagat gcgcgatgcg ccgttatcgc
      961 ccaccagcgg attggtgaca tcacaagcga cgcgaatgac gcaatctttt aagcgcggat
     1021 cgaggccgga aatatcaata tcattcagag tattaagact accgccgcca aaaccaattt
     1081 cattgccgtt ggcgtcgcat aatttcgccc ccagcgcctg taccatgcct gcgccgccat
     1141 catttgtagc gctaccgcca atgccgataa taatgtttgt cgcgccgctc tccagcgcct
     1201 gcaggattaa ctcgcctgtg ccgcgtgaag tggtcacgag tggatcgcgt ttttccgcag
     1261 gtaccagctc cagcccactg gccgccgcca tttcaataaa cgcggttttg ccatcgccgg
     1321 agatccccca actggcattc actttctcgc ccagcggccc tgtaacccag gcgtgacgtt
     1381 cagaaccctg ggtggctgca atcatcgctt ccaccgttcc ttcgccaccg tcggcaaccg
     1441 gaatagaaac gtactgtgca tcaggaaaaa tttcccgaaa tcctttttct atcgcctgcg
     1501 caacctcgct ggcagataaa ctttctttat aagagtctgg ggcgattacg attttcatac
     1561 ctatgcctgt taccacatga cgccggaggg cgtttctctt attcggcctg gattccaggc
     1621 ccggattgca atacgccatc cgggcacgac gtcattaacg agtaacttcg actttcgcca
     1681 gtttttcgta gtagcacacc agggcgctat gatccgccgt tcctaaacca tctgctcgca
     1741 gtgcctgcat catctccata accgcagctg tgagcggcag ttgcgcgccg acgccgtggg
     1801 aagtatccag cgcatttgcc agatccttaa tatgcagatc aatacggaag cccggcttga
     1861 agttgcggtc catcaccatc ggcgctttgg catccagcac ggtactgccc gccagtccac
     1921 cgcgaattgc ctgatacacc aggtccgggt taacgcccgc tttagttgcc agcgttaacg
     1981 cttctgacat cgcggcaata ttcagcgcca caatgacctg attcgccagt ttggtgacgt
     2041 tacctgcacc aattgccccg gtatgcacca cggaacccgc cattgctttc atcaaatcat
     2101 agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac tgacagcgta ccgtcgatgg
     2161 ctttcggttc accgccgctc accggagcat ccagcatatc aatgcctttc gctttcagcg
     2221 cttcgctgat ttcacggctt gccagcggtg cgatagaact catatcgatc aataccgtac
     2281 ctggcttcgc gccttcaata atgccattct cacccagcgc cacctctttc acatgagggg
     2341 agtttggcag catggttatg atgacgtcgc actgttcagc gatcgcttta gccgtagagg
     2401 ctgtttctgc acctgcagca atcacgtcag caatagcttc tgggttacgg tcagcaacca
     2461 ccagcgagta acctgctttc agaaggtttt tactcattgg tttacccata atccccaggc
     2521 caataaaacc aactttcata gtcatatcaa tcatctctct tgttgcggtg gtggttattt
     2581 tttaaaggta tcagccagtt tatgagtggc agagcggaag acgccgagat cgctgccgac
     2641 agccacaaac gtcgcgcccc attccagata acgacgcgca tcggcttcga ccggcgcgag
     2701 gataccgctg ggtttgccgt gcgcgctggc acggttaaaa atgtgctgaa ttgctttttg
     2761 tacatccggg tgtgatgcat tgccgagatg gcctaatgcc gcggccagat cgctggggcc
     2821 gacgaagatg ccgtctacgc cttcggtagc ggcaatggca tcgacgttat ctacgccctg
     2881 ctgactttct atctggacca gaatagtgat gttcttgttc gactgagcga aataatccgc
     2941 cacggtgcca aacatattgg cgcggtgaga aacggagacg ccgcgaatgc cttccggtgg
     3001 gtaacgggtt gatgccaccg cctgctctgc ttcctctttt gtttctacaa aaggaatcag
     3061 gaagttatag aaaccgatat ccagaagacg cttaataatt tccggctcgt tggtcggcac
     3121 tcacactact ggcgcgctgg cgctgccttt caaggccatt aactgcggaa taaacgtgga
     3181 gatatcgttt ggcgcatgtt cgccatccag caccagccag tcaaacccag ccaaaccaag
     3241 aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa ccaatttgta cctgtttcgc
     3301 agccagtgcg gctttgaatt tattcgggaa aacatcgtta ttcatcgctt atacctttgc
     3361 ttatttctgc aattccatac gtttaatgtc gccaactacg aagaggtagc agaccatcgc
     3421 catcagcgct gaacatccca cgaaaaccag tgctgcattg aaggagtgca gttcacttac
     3481 caggtagcca atcaccagtg gagtgacaat ggaggcaaca ttgccaaaga cgttaaagac
     3541 gccgccacag aggccaacaa tctctttcgg cgcggtgtca gaaatcaccg gccagcccag
     3601 agcaccaaat cctttgccaa aaaaagccag cgccatcagc atgaccacca gcgtggtgtt
     3661 gttggtgtag ttacataaga tgatggtgga agccagcaac attcccagca caatcggtag
     3721 cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga taatccgaga agacacctcc
     3781 cagcacgccg cccgcaaaac cacacagtgc cggaatcgag gcgaccagac ccactttcag
     3841 aatcgacatg cctttttcct gcaccagata aatcgggaac caggtgagga agaaccaggt
     3901 gatggtgttg ataaaatatt gtccgaaaaa tacgcccaac atcatgcggt tagagagcaa
     3961 ttgcttgatg taatgcagtt tgggaccgct tgctgccgcg ctgcccggct ttttgtggtc
     4021 catatcgacc accgcgccat tttcagagat aaacttcagc tcttcctcag acatacgtgg
     4081 gtgatctgtc gggttatgaa tcaacttgat ccacagcgcc gtcagcacaa aacaaatcac
     4141 ccccataacg gtaaagacgt gctcccatcc ccaggcgaaa gtcagccagc caagcagagg
     4201 cgaaaagagc gccagcgaga aatattgtgc cgagttaaag atggcggagg cagtaccacg
     4261 ttctttcgtc gggaaccagg cggcgacaat acgggcgttc gccgggaagg atggagcttc
     4321 cgagaatccc agcataaagc gcataaagaa catggagatc cctgcccaag ccagcgggaa
     4381 catatcaaca aagccttgca ggaaggtgaa cagcgaccag aaaaagaggc tgtaggtgta
     4441 aacttttttc gagccaaact tatcaagcaa ccagccgccg gggatttgca tcagcaagta
     4501 ggcccagcca aaagcggaga agatgtaacc catcgaaacc gcacttaact gcaactcttt
     4561 tgccacttcg gtaccagcaa tagacagcgt tgcacgatcg gcgtagttaa cggcggtaac
     4621 aataaaaata atcagtaata ttaaatagcg ggtatgcaca cctttccttt tttcgtcaac
     4681 ggtgtccaga atcattttat ttacctcggg tacttatgct gatttttatt attatgggga
     4741 aggtgttatt tataagtttc atttatgccg taacgacaat gaactcggga attagtataa
     4801 gcagcgcgag aataataatc attgtgcaaa tgctaattta attaatactt tttaaatatt
     4861 attttgagca tatgcacata aggttgcgcg ctaaagcaca gatttgcgct ttaccttgcc
     4921 gggcggcact gcaatcccta aaatgattga cattgatcac atttctgcgt ttaaactcct
     4981 gacattctta tttcacccaa tgaaatcatt tatttttaaa tgagaccagg tcctcatttt
     5041 aataacccct ggctggagaa tattgcacaa tggccaacat cgaaatcaga caagaaacgc
     5101 caactgcgtt ttatataaaa gttcacgaca cagataatgt ggcaattatt gttaatgata
     5161 atggcctgaa agcaggaacg cgttttccgg atgggctgga attaattgaa catattcccc
     5221 aggggcataa agtcgcattg ctggacattc cggctaatgg tgaaattatt cgttatggcg
     5281 aagtgattgg ttacgccgtg cgtgcaatcc cacgcggaag ctggatcgac gaatcaatgg
     5341 ttgtactacc ggaagcgccg ccgttacaca cgctgccact ggcaaccaaa gtcccggaac
     5401 ccttaccgcc gctggaagga tacacctttg agggctatcg caatgccgat ggcagcgtgg
     5461 gcaccaaaaa cctgctcggc atcaccacca gcgtccactg tgtggcaggc gtggtggact
     5521 atgtagtaaa aatcattgaa cgcgatctgc taccgaaata cccgaacgtc gatggcgtgg
     5581 tggggctgaa tcatttgtac ggttgtggcg tggcgattaa cgcaccggca gcagttgtac
     5641 ctattcgtac cattcacaat atttcgctga accctaactt tggcggcgaa gtaatggtga
     5701 ttggcctggg ttgtgaaaag ttgcagcctg agcgcctgct gactggaacg gatgatgtgc
     5761 aagctattcc agtagaaagc gccagcattg tcagtttgca ggatgaaaag catgtcggtt
     5821 ttcagtccat ggtcgaggat attttgcagg tcgccgaacg ccatctacaa aaactgaatc
     5881 aacgtcagcg agaaacctgt ccggcttcag aactggttgt cggcatgcat tgcggtggca
     5941 gcgatgcgtt ttctggcgta acggcaaacc cggcggttgg ctatgcgtct gatctactgg
     6001 tgcgctgcgg cgcaacggtg atgttttcag aagtcacgga agtgcgtgac gcgatccatc
     6061 tgctgacacc acgcgcagtg aacgaagagg tcggcaaacg gctgctggag gagatggagt
     6121 ggtacgataa ctatctcaat atgggaaaaa ccgaccgcag cgccaaccct tcgccgggca
     6181 acaagaaagg cggtctagca aacgtggtag agaaagcgct cggctccatt gctaaatcgg
     6241 gcaaaagtgc aattgttgaa gtgctgtcgc ccggtcaacg cccgactaaa cgcggattaa
     6301 tttacgccgc gacgccagcc agcgattttg tctgtggcac gcaacaggtg gcttcgggta
     6361 tcaccgtgca agtgtttacg accggtcgtg gtacgccgta cggcctgatg gcggtacccg
     6421 tcattaaaat ggcaccccgc accgagctgg cgaaccgctg gtttgattta atggatatta
     6481 atgcgggcac catcgctacc ggcgaagaaa ctattgaaga ggagggctgg aagttgttcc
     6541 actttattct cgacgtcgcc agcgggaaga agaaaacctt ctcggatcaa tggggactgc
     6601 ataaccagct ggcggtgttt aacccagcac cggtgacctg ataattcaat tcgtattatg
     6661 cccgatagca cacgtttatc gggcatattg agtcacagcg aagacgcgtg atccccgacc
     6721 agaatcactt caaccccagc ctttcgcagt ccttccagac tatccgcagg aatgccttca
     6781 tcaacaatga tcatgtcgat acgttgagta tcaatgatct tatgtaaact ggaacgattg
     6841 aatttactgg aatcggtgac cacgatgatc cgttccgcaa cttcgcacat ccgacggctt
     6901 aaacgggctt catcttcatt atgtgtgctg acgccgcgct ccagatcgat cgcatctaca
     6961 ccaagaaaca gcatatcgaa gtggtaattt tgcagcgatt gctcagcctg atcgccgtaa
     7021 aaagattgcg actgacggcg caaatgcccg ccggtcatca gcagctcaac gccttccgct
     7081 tccagcaacg cattagccac gttcataccg ttggtcatcg caattacgtc agtgtacttg
     7141 cgcatcagac gagcaatctc aaaagtggtg gtcccggaat cgaggatcac ccgatgacct
     7201 ggctgaatca actcaacggc agctttcgca acgctgcgtt tcatcgcggt gttcagtgcg
     7261 cttttatctt ccactgatgg ctcgactgac ggcgtcgtgc tatcgcagat caacgcgcca
     7321 ccataggcac gcacagcgat cccctgcttt tccagaaacg ccagatcatt gcggatcgtc
     7381 acagtagata cgccatacaa tgccgacaga tcgttaacct gcacactccc ttgctgtcgc
     7441 agacgctgaa tgatctgttc tcgtcgctcg ctggtgcctg tcactcgctt ctcacctgaa
     7501 gcgtcggtat tactcatagt aagtcctttc gtaaaacttt cgtttcattt cgttttgcct
     7561 attaacgcct ttctattaag caaatgcaag cccaccttgc ccattggcgc aagctactct
     7621 cgtttcagtg actttcatta cgtttctttt gtgaatcaga tcagaaaact attatctttc
     7681 gttttatttt tatctcacca tgacgcagta tcaattgaaa caaaacgaaa gatcaatatc
     7741 gcagtaatct gaactggaga ggaaagtgaa acatctgaca gaaatggtga gacagcacaa
     7801 agcgggcaaa acaaatgcaa tttatgccgt ttgttccgca catccgctgg tgctggaggc
     7861 tgcaatccgc tacgccagtg caaaccaaac gccgttactg attgaagcaa cctccaatca
     7921 ggtagaccag ttcggcggtt ataccggaat gacgcccgcc gattttcgcg gctttgtttg
     7981 tcagctcgcc gactcgttga atttcccgca ggatgcgctg attctgggtg gtgaccatct
     8041 ggggccaaac cgctggcaaa acctgccagc cgctcaggca atggccaatg ccgatgattt
     8101 gattaaaagc tacgttgcgg caggattcaa aaaaatccac cttgattgca gcatgtcctg
     8161 tcaggacgat ccaattccct taactgatga catcgtggct gaacgcgccg cccgtctggc
     8221 gaaagtggcg gaagaaacct gtcgcgaaca ctttggcgaa gccgatctgg agtatgtcat
     8281 tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc ttaagcgagc tggcggtcac
     8341 cacgccggat gccgcccgcg ccacgctgga agcccatcgt cacgcctttg aaaagcaagg
     8401 tttgaatgcc atctggccac gcattattgc cctggtggtt caacccggcg tcgaattcga
     8461 tcacaccaac gttattgatt atcagcccgc caaagcgagc gccttaagcc agatggtcga
     8521 aaactacgaa acgctgattt tcgaagcgca ctctaccgat tatcaaacgc cgcaatctct
     8581 gcgccagctg gtgattgacc actttgccat tctgaaagtt ggcccggcgc tgaccttcgc
     8641 cctgcgtgaa gccctttttt ctctggcggc gattgaagaa gaactggtac cagcgaaagc
     8701 ctgttccggt ctgcgtcagg tgctggaaaa cgtgatgctc gaccgcccgg aatactggca
     8761 aagccactac cacggtgacg gcaacgcgcg tcgtctggcg cgtggttaca gctactcaga
     8821 tcgcgtgcgc tattactggc cggacagcca gattgatgac gctttcgctc atctggttcg
     8881 taatctggcg gattcaccaa ttccgctacc gctcatcagc cagtatctgc cgctgcagta
     8941 cgtgaaagtt cgctccggcg agctgcaacc gacgccacgg gaactcatta taaaccatat
     9001 tcaggacatc ctggcgcagt accacacagc ctgtgaaggc caataagcaa aacaaagagg
     9061 aacatgctat gccaaatatt gttttaagcc ggattgatga acgcttgatt cacggtcagg
     9121 tcggcgttca gtgggtcgga tttgcggggg caaatctggt gctggtagcc aacgatgagg
     9181 ttgccgaaga tccggtacaa caaaacctga tggaaatggt actggcagaa gggatcgccg
     9241 tacgtttctg gacgctgcaa aaagttatcg acaacattca tcgcgccgcc gatcgacaga
     9301 aaatcctgct ggtttgtaaa acacccgccg atttcctgac gctggtgaaa ggtggcgttc
     9361 cggtgaatcg cattaacgtt ggcaatatgc actacgccaa tggcaaacaa caaatcgcca
     9421 aaacggtttc tgtggatgcg ggcgatatcg cagcatttaa cgacctgaaa gccgctgggg
     9481 tggaatgctt cgttcagggc gtcccgacag agcctgctgt ggacctcttt aaattacttt
     9541 gagggattca tcatggaaat cagcctgttg caggcatttg cgttgggcat tatcgccttt
     9601 atcgctggcc tagatatgtt taacggctta acccatatgc accgcccggt ggtcctcggc
     9661 ccgttggtcg ggctggtact tggcgatctg cataccggaa ttttaaccgg cgatacgctg
     9721 gaactagtgt ggatggggct ggccccgctg gcgggcgcac agccgcctaa cgtgattatc
     9781 ggtactatcg tcggcacggc gtttgccatt actactggcg tgaaacccga tgtcgcggtc
     9841 ggtgtcgccg tacctttcgc tgtcgcagta cagatgggga ttaccttcct gttctcggtg
     9901 atgtctggcg tgatgtctcg ctgcgaccgg atggcggaga acgccgatac tcgcggcatt
     9961 gaacgcgtga actatctggc actgctggca ctcggcactt tctattttct ctgcgctttc
    10021 ctgcctatct acttcggcgc ggaacatgca aaaaccatca ttgatgtcct gccgcaacga
    10081 ttaatcgacg gcctcggcgt cgcgggcggc atcatgccag caatcggttt tgccgtgctg
    10141 ctgaaaatca tgatgaaaaa cgtctacata ccctacttca tcctgggctt tgttgccgca
    10201 acctggctca agttaccggt gctggctatt gctgccgccg cgctggcgat ggcgctgatc
    10261 gatctgctgc gtaaatctcc tgaaccgact caacctgcgg cacagaaaga ggaatttgaa
    10321 gatggcatct aatcaaacca ccctgccgaa cgtcagcgaa aatgaagaaa cactgctgac
    10381 tggcgtcaat gaaaacgtgt atgaagatcg gagcattgcc gcggagctga cgaaaaaaga
    10441 tatcaaccgc gtcgcctggc gttccatgct gttacaagcg tcttttaact acgaacgtat
    10501 gcaggcttct ggctggttgt acggtctgct gcctgctctg aaaaagatcc acactaataa
    10561 acgcgacctg gcgcgcgcca tgaaggggca tatgggtttc ttcaataccc atccgtttct
    10621 ggtgacgttt gttatcggca ttatccttgc gatggagcgt tctaagcagg acgttaacag
    10681 tattcagagc accaaaattg ccgtcggtgc gccgcttggc gggattggcg atgcgatgtt
    10741 ctggctaacg ctactgccga tttgtggcgg gataggcgca agcctcgcgc tacaaggctc
    10801 tattcttggc gcagtcgtct ttattgtgct gttcaacgtg gtacacttgg ggctgcgttt
    10861 tggtctggcg cattatgctt accgcatggg cgtggcggcg attcccctta ttaaagcgaa
    10921 taccaaaaaa gtcggccatg ctgcgtcaat cgttggaatg acggtaatcg gcgcactggt
    10981 ggcaacctat gttcgtttaa gcaccacgct ggaaatcacg gcgggcgacg cagtagttaa
    11041 gttacaggct gatgttatcg acaaactgat gcctgccttc ttaccgctgg tctacaccct
    11101 gaccatgttc tggctggtac gccgcggcta gagtccgctg cgcctgattg cggttaccgt
    11161 ggttctaggg atcgtcggta aattctgcca tttcctttaa atacaatgag gtattagatg
    11221 ttaagtatta ttttgacagg gcatggcggg tttgccagtg gcatggaaaa agcaatgaag
    11281 caaatcctcg gcgatcaatc gcagtttatt gccatcgatt ttcccgaaac ctcaagcgcc
    11341 gcgctgctta catcgcagct tgaagaggcc atcgcccaac tggattgtga agacggcatt
    11401 gtttttctga cggatttgct gggcggcacg ccgtttcgcg tagcttcaac gctggcgatg
    11461 caaaaaccgg gctgtgaagt gattaccggc accaatttgc aactgctact ggagatggtg
    11521 ctggagcgcg aagggttaag cggtgaagaa ttccgtgtac aggcgctgga gtgtggacac
    11581 cgagggctga ccagtctggt ggacgagtta ggccgctgcc atgaagaatg tccggtcgag
    11641 gaaggaatat gacacacgtt ctgcgcgcca gaaggctgct gactgaagag ggatggttcg
    11701 atgaccatca gttgcgtgtt gctgacggtg tcatcgcagc aatcgaaccg attccagtgg
    11761 gcgtgactga acgcgatgcg gaacggctct gccccgctta catcgacacc catgtacacg
    11821 gtggtgcggg cgttgatgta atggatgacg cgccggatgt actcgacaag ctggcaatgc
    11881 acaaggcacg cgaaggtgtc ggcagttggt taccgaccac cgtaaccgcg ccgcttaata
    11941 ccattcatgc ggcgctgaaa cgtattgctc aacgttgcca acgcggcgga cctggtgcgc
    12001 aagtgctggg gagttatctc gaaggaccgt acttcacgcc gcagaataaa ggcgcgcatc
    12061 c
//
LOCUS       DAJTQQ010000106        11913 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000106 DAJTQQ010000000
VERSION     DAJTQQ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.106"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..2342)
                     /locus_tag="OO309_003334"
     CDS             complement(<1..2342)
                     /locus_tag="OO309_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5848360.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTEGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNVE"
     gene            complement(2276..2899)
                     /locus_tag="OO309_003335"
     CDS             complement(2276..2899)
                     /locus_tag="OO309_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCR5848361.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2896..4584)
                     /locus_tag="OO309_003336"
                     /pseudo
     CDS             complement(2896..4584)
                     /locus_tag="OO309_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="OO309_003337"
     CDS             complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="OO309_003337"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR5848362.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7507..8229
                     /locus_tag="OO309_003338"
     CDS             7507..8229
                     /locus_tag="OO309_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR5848363.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8357..10213)
                     /locus_tag="OO309_003339"
     CDS             complement(8357..10213)
                     /locus_tag="OO309_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5848364.1"
                     /translation="MRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLE
                     LTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGT
                     IDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLAD
                     VTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGTLLFDSELNGDDSVSDQLVMNGN
                     TAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTL
                     VEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYL
                     NNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLF
                     SGRWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQG
                     AWLQYAWFNNDVSEHEDGVDRYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQ
                     DDFTAANHARVSQSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGST
                     ISDDAVKLRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(10289..11884)
                     /locus_tag="OO309_003340"
     CDS             complement(10289..11884)
                     /locus_tag="OO309_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HCR5848365.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT         2755 a         3303 c         2942 g         2913 t
ORIGIN      
        1 cattcgacat tgaccagttc accaggctgg taatgggttt tgtccgtttt cacccggata
       61 tcaagctgag gaacggcaac tttgatcccg gcgttctgaa aactgtactg accattacgg
      121 gtatacagca ccgaaaaagt gatgttaggc gcaaaggaat tgctcactgg tacccgggct
      181 tcatacaggg tatcgtttaa acgttgtagc gttagccagt ttgccggatg cgaaagcagc
      241 gactgctgtt ccacgcgatc gcgttccagc gtcaataacg cttcatcaat tggctccggg
      301 aaagtaatca gcatcttcgc ggtttcgcct ggctggtaca gcgttttatc cgccacgata
      361 tctaccgtac cagtatgcgc cgtgctgccc ttaccgctga cggcatgact taacccagcg
      421 agaattaagc cgtctttatc gcgtaatgtc agattgtagt tgccaggttt agcgaaattg
      481 acggtaaagg atttgccgcc tgacggtagc tctccgctat ggctcgtgcg gtcttcgaga
      541 cgcaaccatt catacgtaac aggaacctgt tttgaagatt ccagcgcggc ataacggaac
      601 acaaccgact cgccgctatt actgtattgt gcggcggtac ttaaagagta atgcgccagg
      661 ccgcgttcaa tgaggatctc tttggtggtg gtgacgcgat acgccgcgcc gtcactggcg
      721 gagacggtta acaaatagcg gctcggttta tcggcggcgg ggagatttaa cgccacatga
      781 ccgctggcgt cggacaccgt ttcgctgcct tccagcgaca cggggaaacg tccggcataa
      841 cgcaaatcgt taccgaccat tgataattgc tgagcgcgca aacttaactg cacgcgggca
      901 ttttttaccg gctcgccatc cgggtagagc agttgcagtt tgccgctgac cgcttcgcca
      961 gttttgaact cttttttgtc gagagctaaa ccaatctcga aatgtggctt gatgtagttt
     1021 gccacgcgaa aactgctgct atagacctga ttgcggtaag caagacgtaa ctcataacct
     1081 ccggcaacgg cattttctgg caggcggaaa cttccctgcc cgccattgcg cgcatccagc
     1141 gtgacatcga cggtttgcaa cagactgccg ttagcgtcca gcaccgaaag cttcgccggg
     1201 gcgctgacga tgggggatga atgcaacgga tcgcggaact cgcggcccat cactttaaca
     1261 tcgacacgat cgcctgcgcg atatagcggg cgatcggtaa aaatatacaa gcgggtgttg
     1321 tagatttcgc tttcgtagaa gaagttctcg gagacaaaaa cgccgccttc agcatcctta
     1381 cccagaatgt atgaacgttc tggcgatata tgttgtaact gcaaagtacc gctgtcatcg
     1441 gtcaccccgc gggtcatcac gccaagacct tcagtccaca agatctctga gccgggcttc
     1501 gcttcaccct gttttttacc cgcggtccat accagaagct cgttacctga cactttgcta
     1561 agcgccacgg tatcggaaac aaacaccacc gtcgtcgccc gatacccacc taccatcgcc
     1621 tcgacgaggt acagccccgg ctcttgttgg ccgagaggaa tataaatatt acccggctgc
     1681 ggcgagatga aattgctgga tgcgccttcc agttttaccc cttgctgcgg ctcgaccggt
     1741 ttagcctgcc atagtggata acggaactgt tccaccaagg gatatttttt cagcggggaa
     1801 aactggttgt tctgtacata acgggaaggt ttaataatgg cattgccgag atgtaattcg
     1861 ggtaatgcct gagtcacatt ctgccgtgac tgagaagaga aagtacgctg catcacgcgg
     1921 cgagatttgc cgtaccagtt atcccacagc caggtcagcg tattgttcag cccgtcgccc
     1981 agatattgcg gttgcaccac aatgcgatgc aggtttttct gctggcgcaa aaatgccatc
     2041 gggtcaggaa tacgatatag gcgaacgtcc acgccgccgt actcttccat ctgatagcgc
     2101 cgataatcac gccccggcgc ttccagtcgc actttcgcct cttcactgct gctaaaactg
     2161 ctgtcagcaa gcaaaaagaa tgttcccccg gcgggcggcg catagttact ggaaggaagc
     2221 gaatcatcag ctttagcaag ccctgttccc acaagagaca gggacaagca cgctgctatc
     2281 gcgccagaaa atttaaacga taaatgccaa tgaaattggg attggaatcg ttgggtatcc
     2341 atcgggtgtc cttccatgtc ataagttgtt gcagactgac tgcgcgcatt ccgttgtcag
     2401 ttttcgtggc gcttccggtg tggtagatga cgtaacgccc catccagacc attaagtgct
     2461 gggcatcgcc ctgatcgaaa aaaatcatat cgccaggcag cgcctggttt atgtcttggc
     2521 caataaactg gctgttgtac tgaatcaaat taatcgcggt cacgtagggg ccgtttttcc
     2581 cgctcccctg attccagttt tgcgccagtt gacgctgttc aggtgttagc gtcatctctg
     2641 gcggcaaata ctggctagat aaaccgttac tttttagcca tttactgtcg tgaactttca
     2701 gcgtctcgtt cgccgcaaat cgcaccaggc ccgcacaatc ctgctgatac cagcgtggac
     2761 ttggcccctg gcggagctgt tcttgtgcaa tgcgtacaaa ccaggcgcga aacagcctgg
     2821 attgttcgac attcagcatt tcgctatggg caacaacaca acacagccag caaatcagcg
     2881 ccagcagccc gtgtctcata gcggctgcca ggttatgggt agccactgcc aggcgacacc
     2941 gggttccatc tgggccagct tcatgacata acgcggttgt tgagataaag cgtccagctt
     3001 cggcattaat aaagtttgtg cggcgttata aaaaaccggt tcgagattct tcggcagact
     3061 ggtcaacgtt tcgttacgca gcagtttcgc cacgccttgt ggattgatat agagcggaac
     3121 gatgccatca gtgggtatta catccaccat tgccgggcgg gttttattca gtgtttgcag
     3181 ggcgttatta accagcgtgt catcgaggga gaaaagcagc gttttgtttt gcatcgccag
     3241 cgacacacgg aaaaaataat ccgacattaa ttgatcgggt tgcgccgcct gcgctttcgg
     3301 atactgaccg tatcgggaac tcacttcgcg acaccagatt tgcgcttcgc cctgctgagt
     3361 ctggcttacc ggcaaaacac cttctggcgc tttgctttcg tgcgcaccaa tattttgcgt
     3421 aaacagtttc cctggcaatt gcgcctgttc ggcagtgcca tcaaactgac cgacaaacag
     3481 cggggtttgc aattttgagt cttcatacca gcacagcccc gcggcaccgt ctaacgcccc
     3541 attcagcttg tcgttttcct ggctgatgtg cgaaagcatc tcttcggcaa taccgtgtga
     3601 atacggcacc gccacacaga agctggctcc ggcaggcata ctgttccaga ccggcgtaaa
     3661 atcgaaactg gcatctacgc tggcggattc atcatttaac gccacaaaac tgtgccagcc
     3721 gtcgttaccc atttcgaagc gtacgccagc aaaagaaggc attaaccgct ggtagccaaa
     3781 acccagcaac ctggcgctga ccacgatgcg caggcgtact ggcgtttttt cggctgtacg
     3841 ctcttccagg ccaaagctgg cttgccagcg ttttttaccg ctcaacaaat cacttgcgat
     3901 cgccgtggct tcggtatcct gctgatcatc tttaaacaac atatccgtac tggaaaacac
     3961 cagcatcttg tcctgatatg tcgcgaacat cagggcgtta ttgccgttat agcgcaactg
     4021 ataaacagga atagtttcac tatttatctt gatgctactg atttccgttt tgcttaacta
     4081 gctgtcgctg gtagcggcaa acaacaatgg ctccagcagt ttgcttaaac cactgcgctg
     4141 gatcagcacc atgtaatgtg aaagatggcc ttgtttatcg tgccatagcg ccgcctgcgc
     4201 gggctgatct aagagtgacg aaaagagctt atctttcagc gtgagatcgt gttcatagac
     4261 aatgcggcga atgctgcctt caatgcccag acgatcggca tgattctgat aatagaaaac
     4321 gaaatcttcg ctcagaacat cgtggagaaa cggaatggtg aggagatctt tgggaagctg
     4381 gctcagagag tcgctgtcta gaaagaggtc cggctcattg agatcgattt gcagattgtt
     4441 gtgcaccacc agcggcgaca acgttttttc tggcccactg ccagcatatt gcaacgccca
     4501 gacgccagcg gaaagcagtg ctattgcgcc aaaacctaca agaccataga accgccagcc
     4561 tttcgccttt ttttcaccac tcattgccac attccttgtg tatagccagc cattttttac
     4621 gggcacagcc aaactttacc gcgccctaat acgacaaaag cccagacttt gcagcctgga
     4681 cttttcaatt caaacaaggg agatagctcc cttttggcat gaagaagtaa aattattctt
     4741 cttctggctc gtcgtcaacg tccacttccg gagcaatttc atcatcccct tccgcggcac
     4801 tgccgtcgat ggtatccaga tcttcctcgt caaccggttc agcaacacgc tgcagaccca
     4861 ctacgttttc atcttccgca gtacggatga ggatcacgcc ctgggtgtta cggcccacga
     4921 tgctgatttc tgaaacgcga gtacgtacca gcgtaccggc atcggtgatc atcatgatct
     4981 ggtcgcagtc atctacctgc accgcgccaa ccactaaacc gttacgttcg gtaaccttga
     5041 tggagataac ccctttcgtc gcacgcgact tggttgggta ttccgccact gcagtacgtt
     5101 taccgtaacc gttttgcgtt gcggtgagga ttgcgccatc gccacgaggc acgatcagag
     5161 agacgacttt atcgccttca cctaagcgaa taccgcgcac accagtggta ttgcagccca
     5221 tcgcacggac agaagactct ttaaagcgca ccactttacc ttcggcggag aacagcatta
     5281 cttcatcttc gccactggtc agatcaacgc cgatcagctc atcgccgtca accagtttga
     5341 tcgccacttt accggcggta cgcagacggt tgaattcggt gaggacggtt ttcttcacag
     5401 taccgttagc ggtcgccatg aagactttca cgccttcttc aaactcggtc accggcagga
     5461 tcgcggtgat acgttcgtcc tgttccagcg gtagcaggtt gacgatcgga cgaccgcgcg
     5521 cgccacgagt ggcttccggc aactggtaaa ctttcatcga atagacgcga ccacggctgg
     5581 agaagcacag aatatggtcg tgcgtgttcg ccaccagcag tcggtcgata aagtcttctt
     5641 ctttaatacg tgcggcagat ttacctttcc cgccacgacg ctgcgcttcg tattcagaaa
     5701 gcggctgata tttaacgtag ccctggtgag agagcgtcac gaccacatct tcctgggtga
     5761 tcagatcttc caggttgatg tctgcgctgt tggcggtgat ttcagtacga cgtttgtcac
     5821 cgaactgttc acgaaccagc tccagctctt cgcggatcac ttccatcaga cgatcggcgc
     5881 taccaagaat acgcaacagt tccgcgatct gatccagcag ctctttgtat tcgtcgagca
     5941 gtttttcgtg ctcaaggccg gtcagtttct gcaaacgcag atccagaatc gcctgagctt
     6001 gctgttcggt caggtagtac agaccatcac gcacgccgaa ctctggctcc agccattccg
     6061 gacgcgcagc atcgtcgcca gcacgttcca acatggcggc aacgttgccc agctgccacg
     6121 gattagcaac cagcgcagtt ttcgcttctg caggcgtcgg cgcatgacgg atcagttcga
     6181 tgatcgggtc aatgttcgcc agcgccacgg ctaatgcttc aaggatatga gcacgatcgc
     6241 gagctttacg cagttcgaaa atagtacgac gggtcaccac ttcacggcgg tgacgaacaa
     6301 acgccgcgat gatgtctttc aggttcatga tcttcggctg accatggtgc aatgccacca
     6361 tgttgatacc gaaagaaacc tgcaactggg tctgggagta gaggttgttg agcacaactt
     6421 caccgaccgc atcgcgtttc acttcaatca cgatgcgcat accgtctttg tcagactcgt
     6481 cacgcagcgc gctgatgcct tccacgcgtt tttcttttac cagttccgca atcttctcga
     6541 tcaggcgcgc tttgtttacc tgatacggaa tttcgtggac gataatggtt tcacgtccgg
     6601 ttttggcgtc aacttccact tcagcacggg cgcggatata taccttgccg cgaccggtac
     6661 ggtaagcttc ttcaataccg cgacgaccgt taatgattgc cgccgtcggg aagtccggcc
     6721 ccgggatgtg ttccatcagc ccttcaatgc tgatgtcttc atcatcgata tacgccagac
     6781 aaccgttgat gacttccgtc aggttgtgcg gcgggatgtt ggttgccata cctacggcga
     6841 taccggaaga accgttcacc agcaggttag gaattttggt tggcatgacg tcgggaattt
     6901 tttccgtgcc gtcatagtta tcaacgaaat cgaccgtctc tttttcgaga tcggccatca
     6961 gttcatgggc aattttcgcc agacggattt ccgtataacg cattgccgcc gcagagtcgc
     7021 cgtcgatgga accgaagtta ccctgaccgt ctaccagcat gtaacgcagc gagaatggct
     7081 gcgccatacg gacgatcgtg tcataaaccg ccaagtcacc atggggatgg tatttaccga
     7141 ttacgtcacc aacgacacgg gcagattttt tataggcttt gttccagtca ttgcctagta
     7201 cgttcatggc gtaaagtacg cgacggtgta ccggcttcag gccatctcgg acatctggca
     7261 gcgcacggcc aacaatgacc gacatcgcat aatccagata ggagctcttt agctcttcct
     7321 caatgttgac cggtgtaatt tctctcgcaa ggtcgctcat ctaaccgcta tccctctact
     7381 gtatcccgga ttcaaaggtc gcaaattata acacagccgc gcagtttgag gtaaacctat
     7441 acgctttatt cacatccaat gcctgatata ctcgtttgtc ttgccaatta cggagtagaa
     7501 gtgccaatga atgccgaaaa atcgccggaa aaccataacg tagaccacga agagatcgct
     7561 aaatttgaag ccgtcgcctc ccgctggtgg gatcttgaag gtgagttcaa accgttgcac
     7621 cgcattaacc cgctgcgtct gggctatatt gccgagcgtg ctggcggttt atttggcaaa
     7681 aaggtgctcg atgtcggttg tggcggcggc attctggccg agagtatggc gcgcgaaggc
     7741 gcgacggtga ccggtctgga tatgggcttt gagccattgc aggtggcaaa gctgcacgcg
     7801 ctggaaagcg gcattcaggt ggattacgtg caggaaaccg tggaagagca cgcggcaaaa
     7861 catgccgggc agtatgatgt ggtgacctgc atggagatgc tggagcacgt acccgatccg
     7921 cagtcagtgg tcagagcctg tgcgcaactg gtgaaaccag gcggcgatgt ctttttctcg
     7981 acgcttaacc gcaacggcaa gtcatggctg atggcggtgg ttggcgcgga atatattttg
     8041 cgcatggtgc ccaaaggtac gcatgatgtg aagaagttta ttaaaccggc agagctgctt
     8101 ggctgggtgg atcagaccag tttaaaagag cgacatataa ccgggctaca ttacaacccg
     8161 atcactaatt cctttaagct cggccccggc gtggatgtga actatatgct gcacacgcag
     8221 aataagtgag gttgatgttt ggccgcgcca atgcctgatg cgacgcttgc agcgtcttat
     8281 caggcctaca aatgctctcc gtaggccaga taaggcgttt acgccgcatc cggcaattgt
     8341 gatggtcaga cagcgattac catttcaccg tcatcgacaa aaaccctgcc gtctgggcaa
     8401 aatcatcact ccctttctgc cacgccacgc tgccgcgcag ggacactcgc tgactgatat
     8461 tgcccgtgac tcccactttt atttcacccc gtagcttcac cgcatcgtca ctgatagtgc
     8521 tgccatcttc ttcaatttcc gtcgcatggg gatcgtgata gtaattcaga tctaatgttg
     8581 gtgtgacacc aaccgcggta cgccattcgc tgtgtaaacc cagccgcgtc tgaatatcat
     8641 ccccctgcga ttgtgacacg cgcgcatggt tagcggcggt aaaatcatcc tgctgcacgc
     8701 cctgataaat cacctgcgcc tgcggttcaa tcaccacacc acgccccggt aaccactgat
     8761 acccagcttc cagcgaggcg ataatccccg acgaatggta acgatccacg ccatcttcat
     8821 gttcggaaac atcattgtta aaccacgcgt attgcagcca ggccccctgc ttctgattac
     8881 cgtgctgata ccagcttgag gtcagcccaa cggcataacc gtggttctgg ttatcggcgc
     8941 gagttccggt catattcgag cggctgtcgc cctgattatc actgtagcca ccaaccgccc
     9001 caagcatcca ctcgccatcc gtgccccagc gcccgctaaa caagccgccg ctaagctgca
     9061 ccgtagaagt gtcttcctgc tgagccagtt gccctgccgc cgtgtaatga taacgtccgc
     9121 cgataacacg taaattcagc gtctgaccat cgccaccagc gtgatcgcgt cgctccatca
     9181 taaacgcctg atttgccgcc cgcaggttat tgagataacc gccaactttg gcattcagca
     9241 ccggctggta agcaggcgta ggggccgggt cgggggttgg gtcaggatcc ggcgtgggat
     9301 caggatcggg agtcgggtct ggctcaggtg gcgatggcgg cgttacttct tgcgatcgca
     9361 gataccagtc gttgttatct tccactagcg tgtagtcata cgctcccatg ttgacgtagc
     9421 cactgccagc cagactgaac tgtgcattgt tttgaaactg cgtgggatca gctgcgaaat
     9481 caaccacttt aatgcctgtc gatgtcggct caccaatccc tgtaatggag ttaaccacca
     9541 cagttgtgtt gccagcagta ttaccgttca tcaccaattg atcgcttacc gagtcatcgc
     9601 cgtttaattc gctgtcgaat agtagcgtac cgccgccggt ataatcaccg tttaccgtca
     9661 gcgtatcgcc agcgacgcca ttttgcaggc taaccgcacc ggacgtattg gtcagatcac
     9721 cattaaccgt cacgtcagca agataggtca ggctgtcagt ttcaaggttg gtattaagct
     9781 cgcctgtcaa cgtcacgcca tcggtagcat ttaccagcgt cccatcgccg ttaaatagtg
     9841 aggcattaag ggcgacggaa gtatcctgcc cggtcagacg caaaaccgta ccatcgctga
     9901 tgtcgatagt gccgctggaa gtagcatcaa ttgactgaat atcctgatcc gcgccagtaa
     9961 cgaacgttgc cccatcacca acccacagtg acgaagcata aggaaagata tccgcaacat
    10021 cgcctttcag ggtgccttct tcgacacgta ccgccgactg cgttgtaccg ctggcggtca
    10081 gctccagcgt acccgccccc gttttagtaa atgtgctacc ctcatcctga tgctgcccgc
    10141 tgctgtcagc catcagtgcg ccccactgcg tgtctacccc cgcatcaact gccacttcac
    10201 cgtcggcgcg catttcaata tcacgcccat gctcagtcgt cgtagagcga gtatcgacat
    10261 tggcagtaat ctccatcacg ctttcttgtt acttatcggt aaagataacc tggcggttat
    10321 agtgggtatc tccaagctgc gagttgtcgc tcaccatcag cgtaccggag gcgatttgtg
    10381 ttgtgcccaa ataactatta ttgtttgcca gacttacctg cccgtcgctc gcatcgacca
    10441 ccacgtagac gccatctttc ccgtcgccgc cgatatgcaa attgtcgcca cctgcaagat
    10501 ctaccacatc ctgactgccg ataaccgttc cgctgctgcc actgacttca aggccatcat
    10561 tgtatgatgt gccgctctgc caagtggaga aatcagagag atcgagcaca ccgccgttta
    10621 acacgatcga ctgcggatcg tcctggagag cggtaagatc tgccgcgtcg ccttccagcg
    10681 aaagcaccgc accatcatca acgactatat cgccggttag cgccatcgac tgcgcccctg
    10741 ccagcacgta gctgccgttt tgcgcaatgg tgagctgacc agcaccttcg atgatgccag
    10801 caaaactgcc ctgattaaca gtaacgttgc caccagcatc gatatttaaa gtgccattct
    10861 gaaagcccgt caatgcgtgc acaaaagttt gttgggtcga gccaacgttt agctcagcct
    10921 gattctgata ctgatcaata ctccctatcg tcagaccgta gcagtcttgc ggatcgtcct
    10981 ggcaatgcgt atcgccgaca ttcatcaggg agttgctgcg acccagggta acttcaccgt
    11041 tttcaatctg catctcacca gtaaagtcat tgttatctgc attaagtacc agatcgccgg
    11101 aacctgtttt ggtgattaac ccggtaccag caatagagtc aacagctccg tcattctctg
    11161 tattgccgat aaccagcgtt tttccgtcgg caatatcaaa ggtaacttca cttaagccga
    11221 gatacataaa gccacccgcc gcagtggagg gaccatctcc atagcctgct gcgctattgt
    11281 tctcatcgac taacacgccg ccgttctggc tgtagctgtc atcaacagaa atatcaataa
    11341 gatagggagc cgtcgcgcta ttggtatata tcgccccgcc ataaccttcg gctgtgttat
    11401 ttgtaaaggc ggtgttattt attatcgtat aacctgaagg atgcttgctg tcgctattat
    11461 tatcggtaac atcgattgcc ccgccatcgc catcactgta acttgttgat gtatatgcct
    11521 ggttattgtt aaaaacatca tcacttaaat agacgtcatt attaatggta taaattgcgc
    11581 cacctttgcc atcattagcg atattattga tggcgttagt gacacgtaaa tcgacggcac
    11641 cagtatcatt agtaccagaa gaatagattg cgccaccata gccgcctgcg acgttaccgg
    11701 aaaaaataac atcagtaaga ttcagcgttg agttttcttt agcaaatatg gccccgccgt
    11761 tattatattc tcctgtaacg gtgttattgg caaacagagt cattccagtt tcattttctg
    11821 gtaatagtga aaattccgct ccttgttgca aaaatacggc cccaccgctg gcattattgg
    11881 tcatatccga aaaaaccagc cattgcccat cgg
//
LOCUS       DAJTQQ010000107        11686 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000107 DAJTQQ010000000
VERSION     DAJTQQ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.107"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..350
                     /locus_tag="OO309_003341"
     CDS             <1..350
                     /locus_tag="OO309_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5848366.1"
                     /translation="SGNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRL
                     QQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQ
                     NDLLLRATGGVCV"
     gene            complement(467..742)
                     /gene="yjfN"
                     /locus_tag="OO309_003342"
     CDS             complement(467..742)
                     /gene="yjfN"
                     /locus_tag="OO309_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR5848367.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(891..1220)
                     /gene="bsmA"
                     /locus_tag="OO309_003343"
     CDS             complement(891..1220)
                     /gene="bsmA"
                     /locus_tag="OO309_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR5848368.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            1402..2151
                     /gene="yjfP"
                     /locus_tag="OO309_003344"
     CDS             1402..2151
                     /gene="yjfP"
                     /locus_tag="OO309_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5848369.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(2148..2903)
                     /gene="ulaR"
                     /locus_tag="OO309_003345"
     CDS             complement(2148..2903)
                     /gene="ulaR"
                     /locus_tag="OO309_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR5848370.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(3011..4075)
                     /gene="ulaG"
                     /locus_tag="OO309_003346"
     CDS             complement(3011..4075)
                     /gene="ulaG"
                     /locus_tag="OO309_003346"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR5848371.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            4430..5827
                     /gene="ulaA"
                     /locus_tag="OO309_003347"
     CDS             4430..5827
                     /gene="ulaA"
                     /locus_tag="OO309_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR5848372.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            5843..6148
                     /gene="ulaB"
                     /locus_tag="OO309_003348"
     CDS             5843..6148
                     /gene="ulaB"
                     /locus_tag="OO309_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR5848373.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            6158..6622
                     /gene="ulaC"
                     /locus_tag="OO309_003349"
     CDS             6158..6622
                     /gene="ulaC"
                     /locus_tag="OO309_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR5848374.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            6636..7286
                     /gene="ulaD"
                     /locus_tag="OO309_003350"
     CDS             6636..7286
                     /gene="ulaD"
                     /locus_tag="OO309_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR5848375.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            7296..8150
                     /gene="ulaE"
                     /locus_tag="OO309_003351"
     CDS             7296..8150
                     /gene="ulaE"
                     /locus_tag="OO309_003351"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR5848376.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            8150..8836
                     /gene="ulaF"
                     /locus_tag="OO309_003352"
     CDS             8150..8836
                     /gene="ulaF"
                     /locus_tag="OO309_003352"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR5848377.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(8965..9240)
                     /locus_tag="OO309_003353"
     CDS             complement(8965..9240)
                     /locus_tag="OO309_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5848378.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            9567..9962
                     /gene="rpsF"
                     /locus_tag="OO309_003354"
     CDS             9567..9962
                     /gene="rpsF"
                     /locus_tag="OO309_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR5848379.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            9969..10283
                     /gene="priB"
                     /locus_tag="OO309_003355"
     CDS             9969..10283
                     /gene="priB"
                     /locus_tag="OO309_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR5848380.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            10288..10515
                     /gene="rpsR"
                     /locus_tag="OO309_003356"
     CDS             10288..10515
                     /gene="rpsR"
                     /locus_tag="OO309_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR5848381.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            10557..11006
                     /gene="rplI"
                     /locus_tag="OO309_003357"
     CDS             10557..11006
                     /gene="rplI"
                     /locus_tag="OO309_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR5848382.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(11077..11640)
                     /locus_tag="OO309_003358"
                     /pseudo
     CDS             complement(11077..11640)
                     /locus_tag="OO309_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
BASE COUNT         2767 a         2871 c         3088 g         2960 t
ORIGIN      
        1 gttccggcaa tattatgtgc ctggatgtgt tgcgcgttct caataagcaa gcgggcgtat
       61 acgacttatt gtcggaagca tttgtggaag tgaaagggca ggatcgctat tttgatcgcg
      121 cggttcgtcg tttacagcag cagctgcgta agccagctga agaactgggg cgagagatta
      181 ctcatcagct attcctgctg ggctgcggtg cgcaaatgtt gaaatatgct tctccgccaa
      241 tggcgcaggc gtggtgtcag gtgatgttag atacgcgcgg cggcgtacgg ttgtcagagc
      301 agatccagaa tgatttattg ctgcgggcga cggggggagt gtgtgtgtaa gcgtatacga
      361 ctgatgcgac gctggtttcg attaactaaa tgaaatatgt gaaaattgta ggccggacaa
      421 ggcgctcgcg ccgcatccgg cattgttcat ttgctgtaga tgacactcat gcatacagta
      481 tcgcctgtac gcgccagtta tcaggctgct ggtcttcgta catggtaata atgcggtacc
      541 atgacgcacc ttgttcatcg gcctttaacg ccacgatacg ttccacgtcc tgcggatccc
      601 ctgaaatatt gctaactgag atctgaccta tttcattcag gcccgtcacg cagtcagcac
      661 tggcgaattc tgcagactgt gccgtggcgc tcgcaggata agtcactaat tgcagcgagg
      721 gtgaggcaag taattgtttc atcgtcagct ccatttttcg tttccccaca tgttgcaggg
      781 cattccatcg cggtaataaa cgcccaagct tccagccggt aatttattgt gcacaggtaa
      841 acgcataggg acgcaaagca atgtaaagct gactatgtaa gttgtctgat ttatttacgg
      901 tacaaaatgg cttgtgaata ccactgtcct gtcacgatgg tttcgtccac catgacgaca
      961 acgtaataat cagcttttgc agcgacagct tttgctctga tttctgctaa tgcgtcatcc
     1021 ggagaacccc gaaccatcgt gcttacgcta cctattcgct gtaacccttg cgtctggtcg
     1081 cggcgaatct cttgcggatg gtccgttact ggcggtgctg gctgtggcgt accttgcagt
     1141 gcgctacagg cacttaacat caacaccaat aataaactgg caaaccggta aataacgcta
     1201 ttacgtttcc tgctaaccat agtgtagtgc cttattaatt taactttggg ggaatgttcc
     1261 cgaattgtta cctggtacgc gattttcgaa tgaaaacgtg acgaaagcgt aacccacatc
     1321 tcaacattat gaactaatac cgccagcaga aacttgtgct aaggatttct tcatgtcata
     1381 ccagacaaaa aggagagaca gatgattgaa atagaatcac gcgagctggc agatattccc
     1441 gttcttcatg cttatcctgt cgggcaaaaa gataccccgt taccgtgcgt aattttttat
     1501 cacggcttta cttcatccag cctggtgtat agctattttg ccgttgcgct ggcgcaggct
     1561 ggtttgcggg tgatcatgcc ggatgcgccc gatcacggta gccgttttag tggcgacgaa
     1621 gcgcggcggt taaatcaatt ctggcaaatc ttgctacaaa gtatgcagga attcactact
     1681 ttacgtgcgg caatagccga agaaaactgg ctgcttgatg accgtctggc agtcggtggc
     1741 gcgtcgatgg gcgcgatgac ggcactggga attaccgctc gccaccccac ggtgaaatgt
     1801 accgccagca tgatgggatc gggctatttt acatcgctcg cccgttcact gtttccaccg
     1861 ctgatacctg aaacggcagc acagcagaat gaattcaata aaattgtcgc gccgctggca
     1921 gagtgggaag cgacaaacca cctggaacaa cttggggaca gacctctact gctgtggcat
     1981 ggcctcgacg atgacgttgt gcctgccgac gaatcactac gtttgcagca ggccttaagc
     2041 gagacgggac gggataaact gctaacctgt tcatggcagc caggcgtgcg tcaccgcatt
     2101 acgcctgagg cgttagatgc tgccgtgaca tttttccgcc agcatcttta aacacgcaga
     2161 atgctgaccc cttgcgcttc cagttgttgc aggatttccg ggttagcatt tttgccggtg
     2221 atcagcatat cgatttgatc ggcacggcta aaaagcattc ccgcgcgttc gccaatctta
     2281 ctgctatcaa ccagtaccac cagtttccct accacgctca gcatcttctg ctctgccatt
     2341 gctgtcagca tatcggtttt atacaaccct tctgcggtca gcccttttcc gctggtaaac
     2401 atccagtgcc cggcatagag actgttttcg ctgccctgcg ggcttaaagt gatggactga
     2461 cttttgttgt actgtccacc cataatgatc acgctgtcat gttcttgatc gatcaggtaa
     2521 tttgccagcg gtagataatt agtgatgatt tgcactggct tgccacacat ttcccgccca
     2581 agcagaaacg cggtggagcc gcagttgatg actacgcttt cgcccggatt aaccagctgc
     2641 gaggccgctt tagcaatacg tactttttca tcgtgattct gcgcctgatg cagattcatc
     2701 ggcgtccagc gcgggcgctg ttgggtaata gcttctgcgc cattgcgcac ctttttcagt
     2761 ttgccgcttt cgccaagttt attgatatcg cgtcgcgcag tggcaggcga aattcccaga
     2821 cgctcaacga ctttctcaac ggtcacaaag cccaattgtg cgagcatttc caggaggatt
     2881 tgatgtcttt gtgcttcagt catgagctat tccgataaaa tttgattttt ttagatgata
     2941 tttgaaatag ccaggaaata ctacgccgga tagcgcgaac ttctccacgc tatccggtat
     3001 aaacgacaga ttacaggaat gacttgaacg gcagatccgg ttcaatagtg aagcaatcat
     3061 cgaaaccgcg cggatagtgg tactcgaagt tgtctttatc cagcggccag gtaaatttgc
     3121 cgccaacctg ccagataaac ggcttgaagc catacttcag acgatctttt ttgatctccc
     3181 acagcacgcg gatctcttgc ggatcggcct ggaagtttga ccagatgtcg tggtggaacg
     3241 ggataaccac tttcgcattc agggcttcac ccatacgcag catatcggcg ctggtcattt
     3301 tgtcggtaat accgcgcggg ttctcaccat acgatcctaa cgccacatcg atctgatgtt
     3361 cgttaccgtg cttcgcataa tagttagagt agtgggaatc gccactgtga tatagggaac
     3421 cgccaggggt tttgaacagg tagtttactg cgcgatcgtc catgccatct ggcagtacgc
     3481 cagccgcttt ttgatcggca ggcagggtga tcagtgcagt acggtcgaaa gcgtcaagcg
     3541 catgaatttc aatgtctttc acttttacaa catcgcccgg tttgaccacg atgcaacgct
     3601 ctttcggtac gccccagcca atccacaaat caacgcaggt tttcggtcca atgaatggta
     3661 cgtcatcagc acagttctgc atcacggcag cagcgacgtt aacgtcgata tgatcgttgt
     3721 gatcgtgagt cgccagtacc gcgtcgatct ggcgaatcgc aaacggatca agaacaaacg
     3781 gggtggtgcg taggtttggc tgcagttttt tcacgccagc catgcgctgc atctggtgac
     3841 cctgtttcat taacgggtta ccgtgacttt gtttgccagt gccgcaccag aaatcaacgc
     3901 aaacgttggt gccaccttct gatttcaacc agatcccggt gcagcctagc caccacatcg
     3961 caaatgtgcc aggggcgacc tgctcttgtt caatttcttc attcagccag ctaccccact
     4021 ccgggaaagt gctcaggatc caggattctc gggtgatact tttcacttta ctcatcgcca
     4081 tttaccttca tgatagttca attcgaatca acatgtgatt ggttttgatt aatcctgaca
     4141 ctattccttc aggaaggtaa tgaccatttt ttgacttttt gccagggaag ttgttgttga
     4201 ttttgagtgg ggaaagattt aatggaatgt gtagttaaat taactaaatg aatttaaata
     4261 aataattgtt tcgttgtgtg aatcccactc tatccatgtg gaattatttg cgggtcgcgt
     4321 cacatttaat cataaataat cttgttgtga ttacttttga aaattagagt gagtgcacaa
     4381 cattccgggt gtgcggaata cccggttacc tcttcttcag gagatcgtta tggagatcct
     4441 ctacaacatc tttaccgtgt tttttaacca ggtcatgacc aatgccccgt tgttgctggg
     4501 tattgtgacc tgtctgggct acatcctact gcgcaaaagt gtcagcgtta ttattaaagg
     4561 cacgattaaa accataattg gtttcatgtt gttgcaggca gggtccggca tcctcaccag
     4621 caccttcaaa ccggtggtgg cgaaaatgtc cgaagtctac ggcattaacg gcgcaatttc
     4681 cgatacttac gcgtcgatga tggcaaccat cgaccgcatg ggcgatgcct atagctgggt
     4741 aggttacgcg gtgttgttag cgctggcgct gaacatctgt tacgtgctgc tgcgtcgcat
     4801 taccggtatt cgtaccatca tgctgactgg tcatatcatg ttccagcagg cggggctgat
     4861 tgccgttacg ttgtttattt tcggctactc catgtggaca accattatct gcacggcgat
     4921 tctggtttcg ctctactggg gcattacctc caacatgatg tacaagccga ctcaggaagt
     4981 gacggacggc tgcggtttct ccatcggtca ccagcaacag tttgcatcat taattgccta
     5041 taaagttgcg ccgttcctcg gcaaaaaaga ggaaagcgtt gaagacctca aactgccagg
     5101 ctggctgaat attttccacg acaacatcgt ctccacggcg attgtcatga ccatcttctt
     5161 tggtgccatt ctgctctcct tcggtatcga caccgtgcag gcgatggcag gcaaagtgca
     5221 ctggacggtc tatatcctgc aaaccggttt ttccttcgcg gtggcgatct tcatcatcac
     5281 tcagggtgtg cgcatgtttg tggcggaact ctctgaagcg tttaacggta tctcccagcg
     5341 cctgatccct ggtgcggttc tggcgattga ctgtgcggct atttatagct tcgctccgaa
     5401 cgccgtggtc tggggcttta tgtggggcac catcggtcag ctgattgcgg ttggcatcct
     5461 ggtcgcctgc ggttcctcga tcctgattat cccaggcttt atcccgatgt tcttctccaa
     5521 cgccaccatc ggcgtgttcg ctaaccactt cggcggctgg cgtgcggcgc tgaagatttg
     5581 tctggtgatg gggatgatcg aaatctttgg ttgcgtctgg gtggtgaaac ttaccggtat
     5641 gagtgcctgg atggggatgg cggactggtc gattctggca ccgccgatga tgcagggctt
     5701 cttctccatc ggtatcgcct ttatggccgt catcattgta attgcactgg cttatatgtt
     5761 cttcgctggc cgcgcactgc gcgcagaaga agatgcagaa aaacaattgg cagaacagtc
     5821 tgcttaataa ggagttttga ttatgaccgt acgtattctg gctgtgtgtg gcaacggaca
     5881 aggcagttcc atgatcatga agatgaaagt ggaccagttt ttaacccaat caaacattga
     5941 ccacacggta aatagctgcg cggttggcga atacaaaagc gagttgagcg gcgcggatat
     6001 catcatcgct tctacgcaca ttgcgggcga aatcaccgtg accggcaaca aatacgtggt
     6061 tggcgtgcgc aacatgctct ctcctgccga ctttggcccg aaactgctgg aagtgatcaa
     6121 agcgcatttc ccgcaggatg tgaagtaagg acgcgccatg aaattacatg attcgctggc
     6181 ggagaataaa tccatccgcc tgcaggctga agctgagaca tggcaggacg cagtaaaaat
     6241 cggtgttgac ctgctggtgg cggcggatgt tgtcgagccg cgttactacc aggcgattct
     6301 ggatgcggtt gaacagcacg gtccgtactt tgtgctcgca ccaggcctgg caatgccgca
     6361 cggtcgcccg gaagagggcg ttaagaaaac cggttttgcg ctggtgacgc tgaaaaagcc
     6421 gctggagttc aaccacgaag ataacgaccc ggtggatatc ctcatcacca tggcggcggt
     6481 cgatgccaac acgcaccagg aagtgggcat catgcagatc gtcaacctgt ttgaagatga
     6541 agaaaatttc gaccgtttac gtgcctgccg taccgagcag gaagtactgg atctcattga
     6601 ccgcaccaac gcggcagctt aagaaggaat tgaagatgtc attaccgatg ttgcaagtcg
     6661 cgctggacaa ccagactatg gatagcgcct acgaaaccac tcgcctgatt gccgaagaag
     6721 tcgacattat cgaagtgggt accattctgt gcgtgggcga aggcgtgcgt gcggttcgcg
     6781 atctgaaagc gctctacccg cacaaaatcg tgctggcaga cgccaaaatt gccgatgcgg
     6841 gcaaaattct ttctcgtatg tgcttcgaag ccaacgctga ctgggtgacc gtgatttgtt
     6901 gtgcggatat caacaccgcc aaaggtgcgc tggacgtagc aaaagagttt aacggcgacg
     6961 tgcagatcga actgaccggt tactggacct gggaacaggc gcaacagtgg cgcgacgcag
     7021 gtattcagca ggtggtttat caccgcagcc gtgacgcgca ggccgctggc gtggcgtggg
     7081 gtgaagccga tatcactgcg atcaaacgtc tttccgatat gggcttcaaa gtcaccgtca
     7141 ccggtggcct ggcgctggaa gatctgccgc tgttcaaagg cattccaatt catgtgttta
     7201 tcgccggtcg cagcatccgt gatgccgctt ctccggttga agccgcgcgt cagttcaaac
     7261 gttccatcgc cgaactgtgg ggctaaggag cggatatgtt gtccaaacaa atcccgcttg
     7321 gcatctatga aaaagcgctc cccgccgggg agtgctggct ggaacgcctg cgactggcaa
     7381 aaacgttggg cttcgatttt gtcgagatgt cggtagatga aactaacgag cgcctgtcgc
     7441 gccttgactg gagccgcgaa cagcgtctgg cgctggtgaa tgctattgtt gaaaccggcg
     7501 tgcgtgtgcc gtccatgtgc ctttctgccc atcgtcgttt cccgttgggc agcgaagatg
     7561 acgcggtgcg ggcgcagggg ctggagatta tgcgtaaagc catccagttc gcccaggacg
     7621 ttggcattcg cgtgatccag ctggcgggct atgacgttta ctatcaggaa gccaataacg
     7681 aaacgcgtcg tcgtttccgt gacggcctga aagagagcgt tgagatggcg agccgcgcgc
     7741 aggtgacgct ggcgatggag atcatggatt atccgttgat gaactccatc agcaaggcgc
     7801 tgggttacgc gcactatctc aacaacccgt ggttccagct ctacccggat atcggcaacc
     7861 tgtcggcgtg ggacaacgat gtgcagatgg agttgcaggc cggaatcggg catatcgtcg
     7921 cggtgcatgt gaaagacacc aaaccgggcg tcttcaaaaa cgtgccattt ggcgaaggtg
     7981 tggtggattt cgaacgttgc ttcgaaacgc tcaaacagag tggctattgc gggccgtacc
     8041 tgattgagat gtggagcgaa acggcggaag acccggcggc agaagtggtg aaagcgtgtg
     8101 attgggtgaa agcgcgcatg gccaaagcag gcatggtgga ggcggcataa tgcaaaagct
     8161 aaaacagcag gtgtttgaag ccaacatgga tctgccgcgc tatgggctgg tgacctttac
     8221 ttggggcaac gtcagcgcta tcgaccgcga acgcgggctg gtggtgatca agcccagcgg
     8281 cgttgcctac gaaaccatga aagcagacga tatggtggtg gtcgatatga gcggcaaggt
     8341 ggtggaaggg gagtatcgcc catcttccga cactgcgacg catctcgaac tctaccgtcg
     8401 ttacccgtcg cttggcggca ttgtccatac ccactccact catgccaccg catgggcgca
     8461 ggcggggctg gcgatcccgg cattaggcac cacgcacgct gactacttct ttggtgatat
     8521 cccgtgtacg cgtggattaa gcgaagaaga ggtgcagggt gagtatgaac tgaacactgg
     8581 caaagtgatt atcgaaacgc tgggcaacgc cgagccgctg catacgccgg gaattgtggt
     8641 gtatcagcac ggaccgttcg cctgggggaa agatgcccac gacgcggtgc ataacgcggt
     8701 agtgatggaa gaagtggcga aaatggcgtg gattgcgcgc agtattaatc cacaactcaa
     8761 tcatatcgac agctacctga tgaataaaca cttcatgcgt aagcacggtc ctaacgctta
     8821 ttacgggcag aagtaaaaca cgcgctgcgg aaatttcctt cctcgggaga taactggtct
     8881 aattccgcag ccgtttttca aaaaaagccc cctgcgaagg gggcaaagca aactatggca
     8941 atgtttcgtt ggttatacct ggtgctagcg ataaatatcc gcgctggcgt gcatattgcc
     9001 gttactcccc ggttcccgca tcagaattac gtggtagtac gttgcgcctt gcgcatctgt
     9061 ttcttcattt aatgcctgac gtgcttcgct ttcagtggcg aaattatgat tgatataaat
     9121 cacgcctaag ctttgcacat cgtccatatt tctggcctgg tggttattaa tttcaatggc
     9181 tgcccatgta tttgcactta gcaaaagcac agccagaagg gctaaaactc gactgaacat
     9241 agatacctcc tcgacggctg actttgtgtg ctctcctctg tgatgatctt ctgatttaat
     9301 tttaatcaat gataaagaag ttgatggtga ccatttctga tgcagttgtt caaaaaaaca
     9361 ctgtgacgga atgtgatgaa cttcaaatca gcggtttaga ggttatttgc gaaaggggag
     9421 atttatttct gctccgccct tgagtttagc ggggtatgcc agtactataa cgcgtcattt
     9481 ttcagccgac ctttaacacg ttccttgcct ccccgggatt cggctgaccc agacaggagg
     9541 ctgaataatc cgtaaggagc aattcgatgc gtcattacga aatcgttttt atggtccatc
     9601 ctgaccagag cgaacaggtt ccgggcatga tcgagcgcta cactgctgcc atcactggtg
     9661 cagaaggcaa gatccaccgt ctggaagact ggggccgccg tcagctggct tacccgatca
     9721 acaaactgca caaagcacac tacgttctga tgaacgttga agctccgcag gaagtgatcg
     9781 atgagctgga aactaccttc cgcttcaacg atgccgttat ccgcagcatg gttatgcgta
     9841 ccaagcacgc tgttaccgaa gcatctccga tggttaaagc gaaagacgag cgccgtgagc
     9901 gtcgcgatga tttcgcaaac gaaaccgctg atgatgctga agctggggat tctgaagagt
     9961 aatttctgat gaccaaccgt ctggtgttgt ccggcaccgt gtgcaggact ccccttcgaa
    10021 aggtcagtcc atcaggaatt cctcactgcc agttcgtgct tgagcatcgt tctgtgcagg
    10081 aggaagccgg ctttcaccgg caggcatggt gtcaaatgcc cgttattgtt agcggacacg
    10141 aaaaccaggc cattactcac agtataacgg tcggcagtcg cataaccgtt caggggttca
    10201 tttcatgcca caaggcaaag aacggactga gcaaaatggt tttgcatgcc gagcagattg
    10261 aattgataga ttctggagac tagccatatg gcacgttatt tccgtcgtcg caagttctgc
    10321 cgtttcaccg cggaaggcgt tcaagagatc gactataaag atatcgctac gctgaaaaac
    10381 tacatcaccg aaagcggtaa gattgtccca agccgtatca ccggtacccg tgcaaaatac
    10441 cagcgtcagc tggctcgcgc tatcaaacgc gctcgctacc tgtccctgct gccgtacact
    10501 gatcgccatc agtaatcggt cacggtccat taatacgact ttgagaggat aaggtaatgc
    10561 aagttattct gcttgataaa gtagcaaacc tgggtagcct gggtgatcag gtaaacgtta
    10621 aagcgggcta tgctcgtaac ttcctggtac cgcagggtaa agctgttcca gctaccaaga
    10681 aaaacattga attcttcgaa gcacgtcgcg ctgaactgga agctaaactg gctgaagttc
    10741 tggcagctgc taatgctcgc gctgagaaaa tcaatgcact ggaaactgtt accatcgcgt
    10801 ctaaagctgg cgacgaaggt aaactgttcg gttccatcgg tactcgcgac atcgctgacg
    10861 ctgtaactgc agctggcgtt gaagtggcta agagcgaagt tcgtctgccg aacggcgttc
    10921 tgcgtaccac tggcgaacac gaagtgagct tccaggttca cagcgaagta ttcgcgaaag
    10981 tgatcgtaaa cgtagtagct gaataattcg ttattcaacg agacgtaaaa acgccgacca
    11041 ttggtcggcg ttttgctttc tatttttcgt caggcattaa tttcgcaagt agatccattc
    11101 accattctct tgtgtccata agtcaggatg gtttgctggc gttagatgca tacgatcact
    11161 ccttatgtct cggtcctgcg gcactcgctc gaaacctaaa cccgagtaga ttgcggctga
    11221 atttgataag gaacgtagac caattttcaa atcatggggt aaatcagaat acgcgtttag
    11281 caatgcagca atgatgaatc cgagggcatt agaaacgcct gtaacagtgt gaagttggtc
    11341 gatataaaga tcgattgttg gtagttcaga tgagcagcaa ttaggcattt gggcaggaag
    11401 ggttacaacg tatcctatcg tttggccggt atagtctgga gtactataac caggtcccaa
    11461 aacaacctcg tgtattgtgt atttactgtt gggttcgaag gattcgacat cttgcaataa
    11521 atatttcagt tctgcggtta acctcgcacc cgcgtaagct ttattgtatt tctgggcgtg
    11581 aatattatct aatgtagctt catggttcca tccaaaagaa ttatcaccat tcattgttac
    11641 tgaatcgcca cgggtttaac agacacctca gagtcattta agatgg
//
LOCUS       DAJTQQ010000108        11637 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000108 DAJTQQ010000000
VERSION     DAJTQQ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11637
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.108"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            587..784
                     /locus_tag="OO309_003359"
     CDS             587..784
                     /locus_tag="OO309_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848383.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(734..1927)
                     /locus_tag="OO309_003360"
     CDS             complement(734..1927)
                     /locus_tag="OO309_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5848384.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2063..3787
                     /gene="iucA"
                     /locus_tag="OO309_003361"
     CDS             2063..3787
                     /gene="iucA"
                     /locus_tag="OO309_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR5848385.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3788..4735
                     /gene="iucB"
                     /locus_tag="OO309_003362"
     CDS             3788..4735
                     /gene="iucB"
                     /locus_tag="OO309_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR5848386.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4735..6477
                     /gene="iucC"
                     /locus_tag="OO309_003363"
     CDS             4735..6477
                     /gene="iucC"
                     /locus_tag="OO309_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR5848387.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6474..7811
                     /gene="iucD"
                     /locus_tag="OO309_003364"
     CDS             6474..7811
                     /gene="iucD"
                     /locus_tag="OO309_003364"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR5848388.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7817..10012
                     /gene="iutA"
                     /locus_tag="OO309_003365"
     CDS             7817..10012
                     /gene="iutA"
                     /locus_tag="OO309_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR5848389.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10563..10706)
                     /locus_tag="OO309_003366"
     CDS             complement(10563..10706)
                     /locus_tag="OO309_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848390.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10945..>11637
                     /locus_tag="OO309_003367"
     CDS             10945..>11637
                     /locus_tag="OO309_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5848391.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVT"
BASE COUNT         2949 a         2895 c         2985 g         2808 t
ORIGIN      
        1 aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa tcatgactcc
       61 tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag ttttacagga
      121 tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc ccaaaaaaca
      181 taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt caggtgtttg
      241 cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca gtaaaaatac
      301 ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa aagaaacctg
      361 gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc ctgataccca
      421 tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac gcgcagcgca
      481 gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca gagcaacgct
      541 gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg agattacacc
      601 gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca gaacactgcc
      661 tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact ccagtaaggt
      721 gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac acaatggtaa
      781 ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct gttttcgata
      841 ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta gcgagccagc
      901 ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg ggtgttgcca
      961 gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc tgttcggaga
     1021 gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg gccaccggag
     1081 tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact gcggacagac
     1141 ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac tggcgagtaa
     1201 gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat attgcgcaaa
     1261 gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt gtgggctgtg
     1321 gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct gccatcagca
     1381 gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc ggcccgaata
     1441 gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga ttcccttctc
     1501 cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg gtcagcccgt
     1561 aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg gcgctggtag
     1621 ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct cccagagccc
     1681 acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac ggggacgatg
     1741 gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga ctcagatttg
     1801 tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata ccgagaagcc
     1861 cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct tcgatccctg
     1921 gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa attaatttat
     1981 ctgacaataa catttattgt tgataatgag aatcattatt gacataattg ttattatttt
     2041 actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca cagatgtggc
     2101 tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac tgacagaata
     2161 cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt tcagagtggc
     2221 ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg tcacggcatc
     2281 aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata agctacgcca
     2341 tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc tggaaagcca
     2401 tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc gtgaaaaagc
     2461 gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc accctgcgcc
     2521 taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg atatggcacc
     2581 ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg aaagtttgca
     2641 tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc agttactcaa
     2701 cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg aatatctttt
     2761 gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact taggtgaggc
     2821 cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca ccagccgcga
     2881 tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc tgtccgtgaa
     2941 agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct ggcagatgct
     3001 acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg ggctgctcga
     3061 tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc tgctggtgga
     3121 tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac ctgatggcgg
     3181 tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta tcactgtgca
     3241 acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc cgctgtttac
     3301 ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc ttgtccagat
     3361 gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg cttttatgcc
     3421 tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata ttttcaccca
     3481 tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt ttgctgtgac
     3541 cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc gtgtacgagc
     3601 atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact acgtgctgga
     3661 aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc ataacgagaa
     3721 caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc aggctcagga
     3781 ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct gtgaaaaact
     3841 cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc actgtcccgg
     3901 ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg ccgcaccaca
     3961 actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg cgctgacgct
     4021 ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt taccgttatg
     4081 gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag agcgtgagat
     4141 ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt acgatccacg
     4201 tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag aacgtttcac
     4261 ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct cactggaggt
     4321 acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc tgattgggtg
     4381 cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag accgcattgg
     4441 tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg gtgaacagca
     4501 atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt acctgctgct
     4561 ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc agcgcctgtt
     4621 ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc cacataagcg
     4681 ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc tgtaatgaat
     4741 cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc tgagatggag
     4801 tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat taacctgccg
     4861 ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg gattgatgcc
     4921 caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat gcagctaaag
     4981 ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt gtatgccacg
     5041 ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag tgacctgatt
     5101 aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt cgtttttaat
     5161 aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga gtataccaac
     5221 accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg ctgcgataac
     5281 gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt tacacgcttc
     5341 agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc ggtacatccg
     5401 tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga aggcaggatg
     5461 gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg taccctgacc
     5521 aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta caatacctca
     5581 tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc acgctggctg
     5641 caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat ccttggtgaa
     5701 ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc tccctatcgc
     5761 taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct gaaaccggat
     5821 gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca gccgctggca
     5881 ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca attgttccgg
     5941 gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct tattgcacac
     6001 ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct gctgaaagac
     6061 ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc tttgcctcag
     6121 gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga tttgcagacg
     6181 ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct tggcgtacct
     6241 gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa caaacatcca
     6301 caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat tcgcgtggtg
     6361 ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat gctgccgaat
     6421 taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga atcatgaaaa
     6481 aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt gctgcattgt
     6541 cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat ttttcctggc
     6601 atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa gatctggtca
     6661 gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag cacaaaaagt
     6721 tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt tctgactatc
     6781 tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt gaaaatattg
     6841 atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa tattttgccc
     6901 acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg aagcatgtga
     6961 cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg agtggaaaac
     7021 ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat gcattacgcg
     7081 gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat tttaacgcac
     7141 tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca ggcttctccg
     7201 gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca tcggatggca
     7261 tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt gaagttctga
     7321 gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg gaaagtagtg
     7381 gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag agcctggaaa
     7441 gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata cttccctcac
     7501 tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat gacttcactc
     7561 tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc atgcaaactc
     7621 atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt attcttaatc
     7681 gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt cagtggcgca
     7741 gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat acagcaaata
     7801 ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc cgctgcttct
     7861 tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca tagtgtctgc
     7921 cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta tcgaaaatgc
     7981 cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg ctcagttaat
     8041 ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca tgcgtggccg
     8101 cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg acagccgaca
     8161 actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg gcgcgacggc
     8221 cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa aaggtcagcc
     8281 ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca gtaaagatca
     8341 cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg gacgtctttc
     8401 cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca ccctgcttga
     8461 taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa ccggtacgct
     8521 gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa gtcagggaga
     8581 cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga gcagcacacc
     8641 atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc atttgatcag
     8701 cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg tttactaccg
     8761 cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac aggcgacggc
     8821 tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc tgaacagcca
     8881 actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc gctttacctc
     8941 caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga acaaccataa
     9001 gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg ccttcctgca
     9061 atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct atcagtatac
     9121 tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg ctaccgggaa
     9181 ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact ttctgttcaa
     9241 tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt tttcccaggg
     9301 ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg cagcagtgaa
     9361 cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg aaggcgtgaa
     9421 agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga ctcaaatcgc
     9481 ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga ccatcagtgt
     9541 gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc tgatcccgga
     9601 tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga aagtgaacgg
     9661 tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag cttacattaa
     9721 ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg acgtaagcga
     9781 tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta gttggcagct
     9841 tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact ataccactgt
     9901 ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt cactgtacga
     9961 ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct gacggggatg
    10021 cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt tgtttacagc
    10081 cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt tcaccactga
    10141 ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc ctcatttcat
    10201 tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct ggcagatcct
    10261 gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg ctgcaatgaa
    10321 ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa aaagaggtac
    10381 ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct gtcataaagt
    10441 ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc cggcccggag
    10501 gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg ctataggcaa
    10561 agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt ctgaataacg
    10621 cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat cgctaattcg
    10681 tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg aaatattaaa
    10741 cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattag tattgattca
    10801 cataatctct catcattaat gagaacattg tcacatattt acaatataga actgttactt
    10861 ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata ctttgttcat
    10921 tcaataaatg agttgagaga atatatgaat aaaatatact cccttaaata tagtgctgcc
    10981 actggcggac tcattgctgt ttctgaatta gcgaaaagag tttctggtaa aacaaaccga
    11041 aaacttgtag caacaatgtt gtctctggct gttgcaggta cagtaaatgc agcaaatatt
    11101 gatatatcaa atgtatgggc gagagactat cttgatcttg cacaaaataa aggtattttc
    11161 cagcccggag caacagacgt aacaatcact ttaaaaaacg gagataaatt ctctttccat
    11221 aatctctcaa ttccggattt ttctggtgca gcagcgagtg gcgcagctac cgcaatagga
    11281 ggttcttata gtgttactgt tgcacataac aaaaagaacc ctcaggccgc agaaactcag
    11341 gtttacgctc agtcttctta caaggttgtt gacagaagaa attccaatga ttttgagatt
    11401 cagaggttaa ataaatttgt tgtggaaaca gtaggtgcca ccccggcaga gaccaaccct
    11461 acaacatatt ctgatgcatt agaacgctac ggtatagtca cttctgacgg ttcaaaaaaa
    11521 atcataggtt ttcgtgctgg ctctggagga acatcattta ttaatggtga atccaaaatc
    11581 tcaacaaatt cagcatatag ccatgacctg ttaagtgcta gtctatttga ggtcacc
//
LOCUS       DAJTQQ010000109        11519 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000109 DAJTQQ010000000
VERSION     DAJTQQ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.109"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(215..290)
                     /locus_tag="OO309_003368"
     tRNA            complement(215..290)
                     /locus_tag="OO309_003368"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(255..257),aa:Ile,seq:cat)
     gene            849..3101
                     /locus_tag="OO309_003369"
     CDS             849..3101
                     /locus_tag="OO309_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848392.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3437..4369
                     /gene="csiD"
                     /locus_tag="OO309_003370"
     CDS             3437..4369
                     /gene="csiD"
                     /locus_tag="OO309_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR5848393.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4389..5657
                     /gene="lhgO"
                     /locus_tag="OO309_003371"
     CDS             4389..5657
                     /gene="lhgO"
                     /locus_tag="OO309_003371"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR5848394.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDVRIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5680..7128
                     /gene="gabD"
                     /locus_tag="OO309_003372"
                     /pseudo
     CDS             5680..7128
                     /gene="gabD"
                     /locus_tag="OO309_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7136..8422
                     /gene="gabT"
                     /locus_tag="OO309_003373"
                     /pseudo
     CDS             7136..8422
                     /gene="gabT"
                     /locus_tag="OO309_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            8660..10060
                     /gene="gabP"
                     /locus_tag="OO309_003374"
     CDS             8660..10060
                     /gene="gabP"
                     /locus_tag="OO309_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR5848395.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10081..10743
                     /gene="csiR"
                     /locus_tag="OO309_003375"
     CDS             10081..10743
                     /gene="csiR"
                     /locus_tag="OO309_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR5848396.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10744..11193)
                     /gene="kbp"
                     /locus_tag="OO309_003376"
     CDS             complement(10744..11193)
                     /gene="kbp"
                     /locus_tag="OO309_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR5848397.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11277..11435)
                     /locus_tag="OO309_003377"
     CDS             complement(11277..11435)
                     /locus_tag="OO309_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR5848398.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2961 a         2829 c         2874 g         2855 t
ORIGIN      
        1 tgcccgcttg gcatagcggg cattttttcc aggtactttt gaatgagtac tgatgaataa
       61 atacattgca gtggcgtgcc acgtaccaaa acaccagccc tcattcgaaa ccacccaccg
      121 cacttcttcc ttgaaatggc gttagtcatg aaatatagac cgccatcgag taccccttgt
      181 acccttaact cttcctgata catgaatgat gatttggtgg cccttgctgg acttgaacca
      241 gcgaccaagc gattatgagt cgcctgctct aaccactgag ctaaagggcc ttaagtatgc
      301 aataacaata cttataaacc acgcaataaa catgatgatc ttatgatgta aataacagat
      361 ttttatgcgt tcccattcac cttgggtcgt gattacacgc gacataaaac ccgacaccgc
      421 ctccattcgc aaagtcgata ctcgcagtca acaagcaaat gttaataatt agcactatct
      481 atagttatca tcgattcaat gatactttgt aatgattttg tatctaataa tataacttta
      541 ttacattagc tgaagagttt tcgcatcatt atgatatctg ttacttttca ctccataaaa
      601 ataaacttca tatagcaata tattctttca tagatcttat tctgctaatc attagtttcg
      661 tatgcgcgat ttttgacagt tgcctctcca gaccacatcg ataattaaaa aacagattta
      721 aacattatcc ttttccatat agatattgga taaaaatagg acatctgttt gcaattactt
      781 tcacaacaat taaacatttt tatgttttcg tatacatcat attactctac cattagagga
      841 acgttattat gttttctata aaaccaggac ctggaaattt acctatcgac aaccccacat
      901 tgttatcatg gaacattact gatggggatc taaattccaa atttaataca ttagaatatc
      961 taaactgtat aacaaatatt attaatgctt gtggagttta tcctcaggat ttaaaagaca
     1021 gagaaattat atcaactttt cacgcagaaa aagtcattaa tgatctgtta aaaaacgatt
     1081 ataaaatttc cctttctcca gatacaactt accgagagct gaataaagct gcacagagta
     1141 gcattacagc gccagacagg ataggagaag ggaaaacatg ggtttatcaa cgagatacaa
     1201 tggttgaaag aggtgataac agtggtgttc atcagtatgg tccagctgaa catttcactc
     1261 acattatatc tgacaaaccg tccccaaaag ataagtatgt tgcatatgct attaacattc
     1321 ctgactatga gctagcagcc gatgtatata atattaacgt gacgtcacct tccggacagc
     1381 aagaaacatt taaaatacta atcaatccag aacatctacg gcaaacactt gagcgtaaat
     1441 ctcttactgc tgttcagaaa tcacaatgtg aaatcatcac ccccaaaaaa cctggcgaag
     1501 cgattcttca tgcttttaat gccacctacc agcaaatcag ggaaaatatg tctgagtttg
     1561 cacgttccca ttatgggtat atacaaatcc ctccagtgac aactttccgc gccgacggac
     1621 cagaaactcc cgaagaagaa aaaggttact ggtttcacgc ttatcaaccc gaagatcttt
     1681 gtaccatcca taatccaatg ggggatttgc aggattttat cgcattggtt aaagatgcta
     1741 aaaaatttgg tatcgatatc attcctgatt atacctttaa ctttatggga atcgggggaa
     1801 gtggtaaaaa tgacctggat tatccctctg ctgatatacg agcgaagatc agtaaagata
     1861 tagaaagtgg tatccctggc tattggcaag gtcaggtttt gattccattc attaaagatc
     1921 cagtaacaaa agaacgtaaa caaatccatc cagaagatat acatctcact gcaaaagact
     1981 tcgaagcaag taaagataac atctctaagg atgaatggaa aaacctccat gcattaaaag
     2041 aaaagagttt aaatggaatg cctaaaacaa cccccaaaag tgaccaggtt attatgttgc
     2101 aaaatcaata cgttcgtgaa atgcgaaaat atggcgtacg aggtttacgt tatgatgcag
     2161 caaaacactc aaaacatgaa caaatagaaa gatcaataac cccaccgctt aaaaattata
     2221 atgagcgatt acacaatact aacttattta acccaaaata tcatgaaaaa accgttatga
     2281 attacatgga atatctggta acttgtcagt tgaatgaaga acaaatgtca tcgctgcttt
     2341 atgaaagaga tgatttaagc gccattgatt tttcattgct catgaagacg ataaaagcct
     2401 tttcattcgg gggagatctc ctaacccttg catcaaaacc gggttccaca atctcaagca
     2461 tcccatcaga aagacggata ttgattaaca ttaaccacga ttttcctaac aatggcaatc
     2521 ttttcaatga ctttctattt aaccatcaac aagatgaaca attagcaatg gcatatatgg
     2581 ccgctctccc gttcagcagg cctttagttt actgggatgg ccaggtatta aaatcaacga
     2641 ctgaaattaa aaattatgat gggcccacgc gtgtcggtgg tgaggcgtgg cttaataaag
     2701 gttgctctac ctatcagcag ctctacaatg aattccacgc attatatata gataaagcag
     2761 gaatatggag cgcatttgag ggtgtatttg caactaaaaa cgttctggcc tttagtcgtg
     2821 gggattctgt gaacattaat cactctcctc atgatggact agttataata aataaaggaa
     2881 acgaagaagt tgaaggtacc tggcctaaca aattgcaacc tggaatatat aaaaacatgg
     2941 ggagtaatag cgttaacatt attattaata atacccgaaa aattatcccc ccttgtaaag
     3001 tatttacgct tagaggcgga tctctaaata tcaatattcc taggcgtagc gctcttcttt
     3061 tagggaaaac aggagaaccg ccgaactatc tctatttata atttaatgtt atatctgccc
     3121 cgataaaacg gggcagataa tatgtttagt ttactaacgg tcattttgta gtgaagccat
     3181 ttactgtttt ttatcgacca gataatctgt tctctaatgt taactccccc taacctgttg
     3241 ctttagttat tcatttcctg tctcactttg ccttaatacc ctacgttaaa tgttactaat
     3301 ttgttgcttt tgatcacaat aagaaagcaa tatgtcgctt ttgtgcgcat ttttcagaaa
     3361 tgtagatatt tttagattat ggctacgaaa tgagcatcgc catgtcaccc tacatctcat
     3421 aagaggatcg cttctgatga atgcactgac cgccgtacaa aataacgctg tcgattcagg
     3481 ccaggactat agcggattca ctctcatccc gtcggcgcaa tccccgcgtc tgctggaact
     3541 caccttcacc gaacagacga ccaaacagtt tctcgaacag gttgccgaat ggcccgtgca
     3601 ggcgctggag tacaaatcgt ttctgcgttt tcgggtaggc aaaattcttg acgatctgtg
     3661 tgcgaatcag ctgcaaccat tgctgttgaa gaccctgcta aaccgtgctg aaggtgcgct
     3721 gttgatcaat gcggtaggta tcgatgatgt ggcgcaggca gatgagatgg tgaagctggc
     3781 aacggcggta gcgcatctga ttggtcgctc aaatttcgat gcgatgagcg gtcagtatta
     3841 cgcgcgtttc gtggtgaaaa atgtcgataa ctcagacagc tatctgcgtc agccgcaccg
     3901 cgtgatggaa ctgcacaacg acggcacgta tgtcgaagag atcaccgatt acgtgctgat
     3961 gatgaaaatc gatgactggg aacatctgga ccactatttc cgccatccac tggcgcgtcg
     4021 cccgatgcgc tttgccgcgc cgccgagcaa aaacgtcagc aaagatgttt tccatccagt
     4081 gttcgacgtt gatcaacagg gtcgcccggt gatgcgctat atcgaccagt tcgtccagcc
     4141 aaaagatttt gaagaaggcg tgtggttgag cgagctttcc gatgccattg aaaccagcaa
     4201 aggcattctt tctgtacccg ttcccgttgg caaattcctg ttgataaaca acctgttctg
     4261 gctgcacggt cgcgaccgct ttactccgca cccggatctg cgccgtgaac tgatgcgtca
     4321 gcgtggctat ttcgcttacg ccactcacca ctaccagacg catcagtaag cgcaaaggaa
     4381 ttgagcggat gtatgatttt gtgattattg gcggcggcat catcggcatg tcgaccgcca
     4441 tgcaactgat tgatgtttat ccggatgtcc gcattgcgtt gctggaaaaa gagtccggcc
     4501 cggcctgtca ccagacgggc cacaacagcg gcgtgatcca tgccggggtc tattacacgc
     4561 ccggcagcct gaaggcgcag ttttgcctgg cggggaaccg tgccaccaaa gccttttgcg
     4621 atcaaaacgg cattcgctat gacaactgcg gcaagatgct ggtcgccacg tctcagctcg
     4681 aaatggaacg gatgcgcgcc ttatgggaac gcacagcggc gaacggtatc gagcgcgagt
     4741 ggataaacgc cgtggaactg cgcgagcgcg aaccgaatat caccgggctt ggtggcattt
     4801 ttgtgccgtc cagcggcatt gtcagctacc gcgaagtgac ggcggcaatg gcaaaaatct
     4861 tccaggccag aggcggcgag attatttata acgccgaagt cagcgccctc agtgagcata
     4921 aaaacggcgt ggtgatacgc acccgtcagg gcggtgaata tgaagcatcg acgctgatta
     4981 gctgttccgg gctgatggct gaccggctgg tgaaaatgct cggcctcgaa ccgggcttta
     5041 ttatctgccc gttccgtggc gagtatttcc gccttgcgcc ggagcataac cagattgtta
     5101 accacctgat ttaccccatt cccgaccccg cgatgccatt tttgggcgtg catctcaccc
     5161 gcatgatcga cggcagcgtg accgtcgggc caaacgcggt gctggctttc aaacgcgaag
     5221 gctatcgcaa gcgcgacttc tcatttagtg acacgctgga gattttgagc tcgtcgggga
     5281 ttcgccgggt gctgcaaaac catctacgct caggactggg cgagatgaaa aactcgctgt
     5341 gcaaaagcgg ctatctgcgg ctggtgcaaa agtattgtcc ccggctttcg ttaagcgatc
     5401 tccagccctg gcccgccggt gtgcgggcgc aggcagtatc gccggacggc aagctgattg
     5461 acgattttct gtttgtcacc accccacgca cgatccacac ctgcaatgcg ccctccccgg
     5521 cagcgacatc agcaattcct attggtgcgc atattgtcag caaggtacaa acgctgttgg
     5581 caagccagag taaccccgga cgcacgctgc gagcggcacg tagtgtggat gccttacacg
     5641 ccgcatttaa tcaataacct ttgaaaacag gacgtaacaa tgaaacttaa cgacagtaac
     5701 ttattccgcc agcaggcgtt gattaacggg gaatggctgg acgccaacaa tggtgaagtc
     5761 atcgacgtca ccaatccggc gaacggcgac aagctgggta gcgtgccgaa aatgggcgca
     5821 gatgaaaccc gcgccgctat cgacgccgcc aaccgcgccc tgcccgcctg gcgtgcgctc
     5881 accgccaaag aacgcgccac cattctgcgc aactggttca atttgatgat gcagcatcag
     5941 gacgatttag cgcgcctgat gaccctcgaa cagggtaaac cgctggctga agcgaaaggc
     6001 gaaatcagct acgccgcctc cttcattgag tggtttgctg aagaaggcaa acgtatttat
     6061 ggcgacacca ttcctggtca tcaggccgat aagcgcctga ttgttatcaa gcagccgatt
     6121 ggcgtcaccg ccgccatcac gccgtggaac tttccggcgg cgatgattac ccgtaaagcc
     6181 ggtccggcgc tggcggcagg ctgcaccatg gtgctgaagc ccgccagtca gacgccgttc
     6241 tctgcgctgg cgctggcgga actggcgatt cgcgcgggca ttccggctgg cgtgtttaac
     6301 gtggtcaccg gttcggcagg cgtggtcggt aatgaactga ccagcaaccc gctggtacgc
     6361 aaactgtcgt ttaccggtta gaccgagatt ggccgccagt taatggaaca gtgcgcgaaa
     6421 gacatcaaaa aagtgtcgct ggagctgggc ggcaacgcgc cgtttatcgt ctttgacgat
     6481 gccgatctcg ataaagccgt ggaaggcgcg ctggcctcga aattccgcaa cgccgggcaa
     6541 acctgcgtct gcgccaaccg tttatacgtg caggacggcg tgtatgaccg ctttgccgaa
     6601 aaattacagc aggcggggag caaactgcac atcggcgacg ggctggagaa aggcgtcacc
     6661 atcgggccgc tgatcgatga aaaagcggta gcaaaagtgg aagagcatat tgccgatgcg
     6721 ctggagaaag gtgcgcgtgt ggtttgcggc ggtaaagcgc acgaacgcgg cggcaacttc
     6781 ttccagccaa ccattctggt ggacgttccg gccaacgcca aagtgtcgaa agaagagacg
     6841 ttcggccccc tcgccccact gttccgtttt aaagatgaag ccgatgtgat tgcacaagcc
     6901 aatgacaccg agtttggtct tgccgcctat ttctacgccc gtgatttaag ccgcgtcttc
     6961 cgcgtcggcg aggcgctgga gtacggcatc gtcggcatca ataccggcat tatttccaat
     7021 gaagtggccc cattcggcgg catcaaagcc tcgggtctgg gtcgtgaagg ttcgaagtat
     7081 ggcatcgaag attacttaga aatcaaatat atgtgcatcg gtctttaact ggagaatgcg
     7141 aaggaacagc aataaagagt taatgcagcg ccgcagtcag gcgattcccc gtggcgttgg
     7201 gcaaattcac ccgattttcg ctgaccgcgc ggaaaactgc cgggtgtggg acgtttaagg
     7261 ccgtgagtat cttgatttcg cgggcgggat tgcggtgctc aataccgggc acctgcatcc
     7321 gaaggtggtg gccgcggtgg aagcgcagtt gaaaaaactg tcgcacacct gcttccaggt
     7381 gctggcttac gagccgtatc tggagctgtg cgagattatg aatcagaagg tgccgggcga
     7441 tttcgccaag aaaacgctgc tggttacgac cggttccgaa gcggtggaaa acgcggtaaa
     7501 aatcgcccgc gccgccacca aacgtagcgg caccatcgct tttagcggcg agtatcacgg
     7561 gcgcacgcat tacacgctgg cgctgaccgg caaggtgaat ccgtactctg cgggcatggg
     7621 gctgatgccg ggtcatgttt atcgcgcgct ttatccttgc ccgctgcacg gcataagcga
     7681 ggatgacgct atcgccagca tccaccggat cttcaaaaat gatgccgcgc cggaagatat
     7741 cgccgccatc atgattgagc cggttcaggg cgaaggcggt ttctacgccg cgtcgccagc
     7801 ctttatgcag cgtttacgcg ctctgtgtga cgagcacggg atcatgctga ttgccgatga
     7861 agtgcagagc ggcgcggggc gtaccggcac gctgtttgcg atggagcaga tgggcgtggc
     7921 gccggatctt accacctttg cgaaatcgat cgcaggcggt ttcccgctgg cgggcgtcac
     7981 cgggcgcgcg gaagtgatgg acgctgttgc accgggcggt ctgggcggca cctatgccgg
     8041 taacccgatt gcctgcgtgg ctgcgctgga agtgttgaag gtgttcgagc aggaaaatct
     8101 gctgcaaaaa gccaacgatc tggggcagaa gctaaaagac ggattgttgg cgatagccga
     8161 aaaacacccg gagatcggcg acgtacgcgg gctgggatcg atgattgcca tcgagctgtt
     8221 tgaagatggc gatcacagca aaccggacgc aaaactcacc gccgagatcg tggcacgcgc
     8281 ccgcgataag ggcctgattc ttctctcctg cggcccgtat tacaacgtgc tgcgcatcct
     8341 tgtaccactc accattgaag acgctcagat ccgtcagggt ctggagatta tcggtcagtg
     8401 ttttgatgag gcgaagcagt agcaccgtcc ccatgcagga ggcgacgctg cgcgtcttgt
     8461 ccggcctacg agaatccaag tcggataagg cgtttacgcc gcatccgaca atctgtacgt
     8521 gaacaggaat aaatctatgt cggccggata aggcggagcc gctctccggc aaaaagaatc
     8581 aataacaatt aaacgcgtga cccgacgcgg gaaatgtcgg ggcgctctcc caagtgacac
     8641 actttcgaga ggattcagga tggggcaatc atcgcaacca cacgagttag gcggcgggct
     8701 gaaatcacgc cacgtcacca tgttgtctat tgccggtgtt atcggcgcaa gtctgtttgt
     8761 cggttccagc gtcgccatcg ccgaagcgga cccggcggta ttactggcct atctgttcgc
     8821 cggattactg gtggttatga ttatgcggat gctggcggaa atggcagttg ccacgcccga
     8881 taccggttcg ttttccacct atgccgataa agccattggc cgctgggcgg gctataccat
     8941 cggctggttg tactggtggt tttgggtact ggttatccca ctggaagcca acatcgccgc
     9001 catgatcctg cactcgtggg ttccaggcat tcccatctgg ttattttccc tcgtcattac
     9061 cctcgcctta actggcagta atttattaag cgttaaaaac tacggcgaat ttgagttctg
     9121 gctggcgctg tgcaaagtca tcgctatcct ggcctttatt ttccttggtg cagtcgcaat
     9181 tagcggtttt tacccttatg ccgaagtgag cgggatctca agattgtggg atagcggcgg
     9241 ctttatgccc aacggtttcg gtgcggtatt aagcgcgatg ttgatcacca tgttctcgtt
     9301 tatgggcgca gaaattgtca ccattgccgc cgcggaatcc gacatgccgg aaaaacatat
     9361 tgtccgcgcc accaactcgg ttatctggcg tatttctatc ttctatttgt gctctatttt
     9421 tgtcgtcgtg gcattaatac cgtggaatat gccggggctg aaagccgttg gatcttatcg
     9481 ctcggttctg gaattgctca atattcccca tgcgaaatta atcatggact gtgtgatatt
     9541 actttccgta accagttgcc tgaactcggc cctgtatacc gcctcaagga tgctctactc
     9601 cttaagccgt cgcggtgatg ctcccgcggt aatgggtaaa atcaaccgca gtaaaacccc
     9661 gtacgtggcg gtgttactct ccaccggcgc agcattctta acggtggtgg tgaactatta
     9721 cgcgcctgcg aaggtattta aatttctgat cgacagctcc ggcgctatcg ccctgctggt
     9781 ttatttagtc atcgccgttt cacagttgcg gatgcgcaaa attctgcgag cagaaggaag
     9841 cgaaattcgc ttgcgaatgt ggcttgatcc ctggctcacc tggctggtca tcggctttat
     9901 tacctttgtg ttggtagtga tgctattccg tccggcgcaa cagttagaag tgatctccac
     9961 cggcttatta gcgataggga ttatctgtac cgtgccaatt atggctcgct ggaaaaagct
    10021 ggtattgtgg caaaaaacac ccgttcataa tacgcgctga taatgatcag gagtcacacc
    10081 atgaccatta cgtctctgga tggctatcgc tggctgaaga acgatattat tcgcggtaat
    10141 tttcaaccgg atgaaaaatt acggatgagt ttactgacat cgcgttatgc acttggcgtt
    10201 gggccgttgc aggaagcact ttctcaactg gtggcggaac ggctggtcac ggtggtgaat
    10261 caaaaagggt atcgggtggc gtctatgtca gagcaggagc tgctcgatat tttcgacgcc
    10321 cgcgccaata tggaagcgat gctggtcagt ctggcgattg cccgcggtgg cgatgaatgg
    10381 gaggcagacg ttctcgcaaa agcgcatctg ctcagtaagc tggaagcctg tgacgccagc
    10441 gagaaaatgc ttgatgagtg ggatctgcgt catcaggcgt ttcatacagc aattgtggcg
    10501 ggctgtggtt ctcactattt gctgcaaatg cgtgaacggt tgtttgatct ggcggcgcgt
    10561 tatcgattta tctggctgcg gcgaacggtg ctttcggtgg aaatgctgga ggataaacac
    10621 gatcagcacc agaccctgac tgcggcggta ctgccgcgag atacagcgcg cgccagtgag
    10681 ttaatgcgcc agcatttact gacgccaatt cccattatcc agcaggcgat ggctggcaat
    10741 taactactct tccggaatac gcaacacttg ccccggataa attttatccg ggctttttag
    10801 catcggttta ttcgcttcga agattttatt gtacagatta gcgttaccgt agacctgttt
    10861 ggaaatggca ctcagagtgt cgccagactt aacggtataa aactggctgg cagtggctgg
    10921 tgtcgccgtt ttcacctgat catcaacact ggcaataccg gaaatattcc ccaccgcaac
    10981 aaggattttc tccttcgcct cctgactcag gccgtcacca gtgaccgtcg ctttgccgtc
    11041 ggcaatttga atgttcactt tatcggcatc aggtataccg gttttgctca gatgctcctg
    11101 caccttcttc gcctgatcgt ctttatcgtg ctgacctgta accgcatccc agagtttttc
    11161 tccggcatct ttaacaaaat tgaacagacc catagctacc tcattcgtta acggaaacac
    11221 tcagaaagtg tagcagagga acgcggcgcg ttaacgccgc gggggtgagc acaggtttaa
    11281 tcgcgggttt gcacccagaa cgcgtgaatc aaaccgggga tatacccaag cagtgtcagc
    11341 agaatattaa taatgaacgc ccaaccgaac cctttaccga gcagcacgcc gatcggcggc
    11401 agaatgatgg tgatgacgat tctccagaaa cccatatgta ctccctataa gaaaattact
    11461 cattgtttaa aaagagattt tatctcttaa gcgtagtaat tttagcggag cctgccagt
//
LOCUS       DAJTQQ010000110        11489 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000110 DAJTQQ010000000
VERSION     DAJTQQ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.110"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(53..697)
                     /gene="cheZ"
                     /locus_tag="OO309_003378"
     CDS             complement(53..697)
                     /gene="cheZ"
                     /locus_tag="OO309_003378"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR5848399.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(708..1097)
                     /gene="cheY"
                     /locus_tag="OO309_003379"
     CDS             complement(708..1097)
                     /gene="cheY"
                     /locus_tag="OO309_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR5848400.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1112..2161)
                     /gene="cheB"
                     /locus_tag="OO309_003380"
     CDS             complement(1112..2161)
                     /gene="cheB"
                     /locus_tag="OO309_003380"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR5848401.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2164..3024)
                     /gene="cheR"
                     /locus_tag="OO309_003381"
     CDS             complement(2164..3024)
                     /gene="cheR"
                     /locus_tag="OO309_003381"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR5848402.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3043..4644)
                     /gene="tap"
                     /locus_tag="OO309_003382"
     CDS             complement(3043..4644)
                     /gene="tap"
                     /locus_tag="OO309_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCR5848403.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(4690..6351)
                     /gene="tar"
                     /locus_tag="OO309_003383"
     CDS             complement(4690..6351)
                     /gene="tar"
                     /locus_tag="OO309_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCR5848404.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6496..6999)
                     /gene="cheW"
                     /locus_tag="OO309_003384"
     CDS             complement(6496..6999)
                     /gene="cheW"
                     /locus_tag="OO309_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR5848405.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7020..8984)
                     /gene="cheA"
                     /locus_tag="OO309_003385"
     CDS             complement(7020..8984)
                     /gene="cheA"
                     /locus_tag="OO309_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCR5848406.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(8989..9915)
                     /gene="motB"
                     /locus_tag="OO309_003386"
     CDS             complement(8989..9915)
                     /gene="motB"
                     /locus_tag="OO309_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR5848407.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRIGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(9912..10799)
                     /gene="motA"
                     /locus_tag="OO309_003387"
     CDS             complement(9912..10799)
                     /gene="motA"
                     /locus_tag="OO309_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR5848408.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(10926..11489)
                     /gene="flhC"
                     /locus_tag="OO309_003388"
     CDS             complement(10926..11489)
                     /gene="flhC"
                     /locus_tag="OO309_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR5848409.1"
                     /translation="MVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLYKELRG
                     SPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLYLEQCP
                     QAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLCQPPSR
                     AVKRRKLSQNPADIIPQLLDEQRVQAV"
BASE COUNT         2606 a         3242 c         2810 g         2831 t
ORIGIN      
        1 ccctgctatc ggcttggcat catcgcgcag gccgaagttc aggcaataca aatcaaaatc
       61 caagactatc caacaaatcg tccacctgat cctgactggc taccacgccg gctttgctgg
      121 tatcgacctg aggtccatta agcaaactct ggttttcacg ttttggacgc gactcctgtt
      181 ccgggatgtt ttccaacagc accatcagca actggcgttc gatctcctga atgacatcca
      241 tcatccgctt aataacctgc ccggtgagat cctgaaaatc ctgcgccatc atgatttcca
      301 gcagttgcgc gttagtaaag ctggtatgcg cgggtacatc tgccagaaat tgtcgtgtat
      361 ctgttaccag ttcacgggcg tcggcaaggt caatcggatc ggcaaaccag tcatcccaac
      421 gttgggttaa cgcttttgct gatttctcca tttgatcctg atgcggttgt gacgcctcaa
      481 cactgttcag cgcccgctcc gcagcctggg cggtcatctg cacaacatag tacaaacgat
      541 cgcgcgcatc ggggatggct tccgccgctt cggcaatggc ctgaaccagc cccagttccc
      601 gcaaactgtc gcgcagcata tgcgtcaggc tgccgatgcg cgcaatgata tcgccagctg
      661 aatgctcgtc agcaggtttg attgatggtt gcatcatagt cgcatcctca catgcccagt
      721 ttctcaaaga ttttgttgag tttttcctcc agcgtcgcgg cggtaaatgg cttcaccaca
      781 tagccactgg cccccgcttg cgccgcagca atgatgttct ctttcttcgc ttctgcagtc
      841 accattaaca ctggcaatgc cgacatcgcg ccatccgcac gaattgtttt cagcaattcc
      901 aggccatcca tattgggcat gttccagtcg gagataacaa atccataacc gcctgcctgc
      961 aacttattga gagcgtcgac accatcttcc gcttcctcaa cattattgaa tcccagctct
     1021 ttcagcaggt tacgcactat gcgtcgcatg gtggaaaagt catccacaac caaaaattta
     1081 agttctttat ccgccatttc acactcctga tttaaatacg tatcgcctgt ccggcactaa
     1141 ttgttgccaa catttgctgg cttacctggc taagatcgac cacttcgcag acaccaccca
     1201 tattgatggc ctcgcgcggc atgccgaaca ccacgcaact tgcttcgttt tgcgcaaggg
     1261 tccatgcccc cgcctgacgc atcgccaaca ttcccgccgc gccgtcgttg cccataccgg
     1321 tcaggatcac cccaaccgca ttacgccccg cctgtttggc gacagaatgg aacaacacat
     1381 ctaccgaagg ccgatgacgg ttaaccgccg ggccgtcgtg aattttgatt tgataatttg
     1441 cgccactacg cgccagttcc atatgccgat cgcccggcgc aatataggca tgccccggca
     1501 agacacgttc tccgtcttcg gcttctttaa ccccgatctg gcaaagctta ttaagtctgt
     1561 cggcaaaaga gcgggtgaaa ccgggcggca tatgttgggt aattaacaat gccgggctgg
     1621 aaagcggcaa cggttgcagt acgtgacgaa ttgcctcagt tccacccgtt gaagcaccaa
     1681 tcgcaatcag tttttcagaa ctcaacaacg gccctgcctt cagcgttgtc ggtgctgaca
     1741 atggcttatg tgctgcaagg ctcgcctttg ctgccgtacg caccttttca gcaatcattt
     1801 cgctatacgc cagcattcct tcgcgaatac ccagttgcgg tttggtgaca aaatctattg
     1861 cccccagctc cagcgcgcgc agcgtgactt ctgatccttt gccggtcaag gaagaaacca
     1921 tcacaacggg cattggacgc aaacgcatta atttttcgag gaaatccagt ccgtccatcc
     1981 gcggcatttc aacatccagc gtcagcacat cgggattgaa tttcttaatc aagtcacgcg
     2041 caaccagcgg atcaggcgcg gtcgccacca tttccatgtc gctatggctg ttgatgattt
     2101 ctgtcatgat ctggcgcatc agtgccgaat catcgacaga taacaccctg attttgctca
     2161 tcgttaatcc ttacttagcg catacaccgt ctgaccacgc agcgtgaagc ggcgctcgag
     2221 gtggctaaag ttttcagagt gacccgcaaa cagcaatccg tcgggtttaa ggagcggaac
     2281 aaagcggcgc aaaatctcct gctgggtagt ttgatcgaag tagatcatga cgttacgaca
     2341 gaagatcgca tcaaacggcc ccggcacggt gtactgtttc gccagtaaat tcagcggggc
     2401 aaaatcaaca tagttcgcca gctcctgacg cacgcgtacc agcccttcat gcggtcccgt
     2461 ccctcgcatg aaataccgtt gcagttgctg cggcgtcagg tttttcaact cttcatggcg
     2521 atagataccg cttctggctt tttccagcac ttcggtgtcg atatcactgg caaacacttt
     2581 ccagcgtccg ggcacggtgc ccaatgtgtc agccagagtc atcgcaatgc tgtacggctc
     2641 ttcgccggtc gaagccgccg cgctccatac gcgatcctcg ccagaacggc gacgtgcgtg
     2701 atccgcgagc agagggaaat gatgagcctc acggaaaaat gccgtcagat tcgtggtcag
     2761 cgaattgata aacgcctgcc actcaccgct gtgctgatta gattccagca agttcagata
     2821 atgaccgaaa tccgtcagtc ccagagaacg caaacgacga accagtcggt tgtaaaccat
     2881 gtcgcgttta tggtcagcca gaacgatccc ggctcgttga tagatcaatt gacttatccg
     2941 ccgaaaatgc gcgtcggaaa gcgccaggcg ctcggtcatc tgtaacaata aagacgtttg
     3001 cccacagggc agagatgaag tcatagcgcc ttctcaatca cttcaggata ccactggcgc
     3061 aatttgtaac tgcgccgact catgtcgtgc tacttcatgt tcttcaaggg taaataccgc
     3121 cacacgcgac gaaagatggt cggcctgatt cgccagttgt tccgttgcca ccgccgcttc
     3181 ttctaccagc gaggagttct gctgagtcac ctgatccatc tggctgacag cctgtgcaac
     3241 ctgctcaatc ccccgccgtt gttcttccga cgccgaagca atttctccca taatgtcgtt
     3301 cacgcgggtc accgaactga caatatcggt catggtcgcg gcggcgttat tcaccagttt
     3361 cgaaccctgc tggacacgat tgactgactc ttcgatcagc cctttgatct cttttgctgc
     3421 ctgcgcgcta cggctggcaa gattgcgcac ttcacctgcc actaccgcaa aaccacgccc
     3481 ctgctctccg gcgcgagccg cttccactgc cgcattcagg gccagaatat tggtctggaa
     3541 agcaattccg tcgataacgc tgataatgtc gccaattttt tgcgagctgg tggcgatctc
     3601 ctgcatggtg tgagtcatgg tactgacctg aacaccgccc gcctgcgccg ttgtcgcggc
     3661 attttttgcc agttccgacg cctgtcgtgc gttatcggcg ttttgcccta ccgtggcggt
     3721 taattgctcc atactggcgg ccgtttgtgc cagcgatgcc gcctgctgtt cggtacgact
     3781 tgagagatcg ttattgcctg cgacaatctc cgcgatccca atgtgcatct catggcttcc
     3841 cttacgcaca tcactcaccg tcccacgcaa agcctgctgc atggtcttca ggctggcaaa
     3901 aatggcggtg atctcattac gaccatatac cgcaatcgga cgcgccagat tacccgcagc
     3961 aatgctgtca aaatggctac cgataatggc cagtggttga acaatcatct tgcgcgtcca
     4021 ccacagcgca ctgctgatgt agatcgccgc aacaataatc atgctgataa acaccagcgc
     4081 cgaaatctga tagttacgct ggctttgcgc actggcggct tccagcacat ggttgatctc
     4141 cagctgccag gcctcaaagt taacgtcaaa cgcattctgt gatccctgca ccggcgcagt
     4201 gaggaaatcc gaaagttgat tactttcaag ccaggtcgcc tgatgttcga gatcgttgtg
     4261 ccagcgggca aaacttttct ccgtctcttt ttgcaatgcc ctgacgtgct cgttgcccgc
     4321 agtcatcgcc ataaaacttt taaacagcgt ggtggattgc gtcagactgg cgcgcgccgt
     4381 cgtcatcaac gttttaatgt catccgccgg atagctaagc gctgtcagag tgcctgcttt
     4441 gttcagcgcg gtactggcct gtaacattac cgcccgagtt tgcgctaatg ccgcacgttg
     4501 ctgattgctc tgctcgccgt gattcagtcg ttgcaaatcg tcgcgaaatg cccaaaaaga
     4561 catgccgtta ctgccaatct gcaagatccc gcagagaatc aaaattaaaa acagcgtggt
     4621 cgaaattcga atacgattaa acatcaacgc tccccatcag gcggcaatga ccgcgttagt
     4681 aaaaactcgt caaaatgttt cccagtttgg atcttgttca gtaattcgca gtcgtggctg
     4741 tgccggtggt tgctcactgg caggacggga tggtgtttgc ggtttattgg tgagtgggct
     4801 ggctgccaga cggaacgcgg aaaccgcttg cgttaaacga ctcgcctgtt cttccagcgc
     4861 agctgcggcg gcagctgatt cctgcaccag cgatgcgttc tgttgcgtga cgcgatccat
     4921 ttccgaaacc gccaatgcga cttgatcgat gccacggctc tgttcatccg atgccgatgc
     4981 aatctcgccc ataatgtcag tcacgcgagt gacagcattg acgatattgt tcattgtttc
     5041 cccggcgctt tcgaccagca ccgaaccggt atcaatgcgc gagacggagt cttcaatgag
     5101 ggctttgatc tcttttgccg ccagggcgct gcgactggca agattacgca cttcacccgc
     5161 caccacggca aaaccacggc cctgttcacc cgcacgcgcg gcttcaaccg cggcattcag
     5221 cgcgaggata ttagtctgga aggcaatacc gtcgataacg ctgataatgt cggcaatttt
     5281 cttcgaacta tcggcgatct catgcatcgt tttcactacg ccatccacca ctttgccgcc
     5341 gtgctgggcg gtgtcggagg cactttgcgc cagttgcgag gcctggcggg cgttatcggc
     5401 gttttgcttc actgtcgcgg tgagctgctc catgctggcg gcagtttctt ccagcgcgga
     5461 tgcctgctgt tcagtacggg aggaaagatc ggtgttgccc gccgcaattt cacgggtacc
     5521 ggcatagatg gcatctgaac cttcgcggac atgagtgacg gtgtcagtca aagagcgttg
     5581 catatgtgaa acgctctgcg ccaggtcacc catttcactg cgcccgtcaa tggtcagggt
     5641 attcgccagg ttaccaccgg cgatttcgca aatgtgagca ataatttttg ccagcggagt
     5701 aagcaacata cggcgaatgc cgtaccacgc caccagcaga atcaatacca ccaccagcgc
     5761 gataaccgcc agttgccact gggcaaatcg gtaatcatct gcgttgtcag tgacgatatc
     5821 gcgatacagt ttttcactgc tgagggcgta ctgagcaaac gcttcgccca ttgcattttg
     5881 cattccctgg gttggctgag cgaaataagc tccagtattg ccataatcaa gataatcaat
     5941 cagttcagtt aacgctgtgt aatagttttt atatttttca tcaaaattac ggctggtagc
     6001 gaccatttca ggtaacggtg ccatgctttt gaatttttta taatgcgtcg cggcctgggc
     6061 caatgttttc ctggcgctat cgagcaattc aactttggcg ttactttgtt gattggagga
     6121 atccatcatc atccgcaccg ctgaacgact caggttaatg cgcgtttgca gcattaaatc
     6181 ccaggttgac gtcagctcgc cctgctgttc ccgtaattga ttggaaacca caaagctctt
     6241 ctggctatgg tgaagggaag aaaaaaacaa actgccggaa ataagctgta acagtgcgaa
     6301 tacccccagc accattacca acagcgtgac tacgcggata cggttaatca taaggcacct
     6361 tcctgaaaac aagttgatct cgttatcggc aagaaggggg gaaactttat tgctgatgcc
     6421 acccgccgcg aaattgaaat aaaaaacccg atgcgcagat catcgggttc atttcaattg
     6481 aggaaatcgg gagaattacg ccacttctga cgccgcgcta tctaacagcg ccatctcttc
     6541 gctgttcagc agtttttcga tgttcaccag aatcaacata cggtcgccca gtgcgcccag
     6601 tccagtgaga tattctgttg aaagcgtcac ggcaaactct ggtgccggac gaatttgctc
     6661 cgccgtcaat gaaagcacgt ctgaaacgcc gtcaaccacg atgccgacca cccgctgtcc
     6721 gagattcagg acgataacta ccgtgttgtc gttatagtcc acatccacct ggctgaactt
     6781 aattcgtaag tcaacaatcg gcacaataac gccgcgcaga ttcgtgacgc ctttgataaa
     6841 cgctggcgtg ttcgcaatcc gtgttacctg atcgtagcca cggatctcct gcactttcag
     6901 gatatcaata ccgtactctt catcaccaag ggtaaatacc agaaattcct ggcctgacgg
     6961 ctcgctggcc agctttgtta cattcgtcat accggtcata ttgttacctt tttactcatt
     7021 caggcggcgg tgttcgccat acgttgttcg cggtttatcg cctgcaaggc ggagacatca
     7081 acaatcagtg ccacgctgcc gtcgccaaga atggtcgcag cagaaatgcc ggggactttg
     7141 cgatagttac tttcaaggtt tttaaccaca acctggtgtt gaccaattaa ttgatccacc
     7201 agcaaggcgt agcggcgacc gccactttgt aagatcacca caattccctg ggtggcttcg
     7261 gttttcgcgc ccgcgacgtt gaacactttc cacagttcga cgatgggcag atattcaccc
     7321 cgcacttcca gcacccgctc gccgccggcc agtggatgga gatcggcttc acggggttgc
     7381 agtgattcca taacagcatt cagcggcaga atgaaaactt catccgcaac gcgtacggac
     7441 atgccgtcga ggatggccag cgtcagcggc agtaaaatgc ggatcgtagt gccagtaccc
     7501 tgcttcgact ggatttcgac atgaccgccc atctcctgga tattacgttt aacgacgtcc
     7561 atgccgacgc cgcgcccgga gacgtcggtg acctgctctg ccgtggagaa gcctggtgca
     7621 aatatcagca ttgcaacttc gtcgtcgctc atgttttcgc tgacagtcaa accttgcgag
     7681 gccgcttttg ccagaattcg ctcacggttt agccccgccc catcgtcggt cacttcaatg
     7741 caaatgttgc cgccctgatg ttcggcagac agaattaaat ttccgacgct gtttttacct
     7801 gcggcgagcc gtttttctgg cagttcaata ccgtgatcga ggctattgcg taccaggtgg
     7861 gtcagcgggt cgataatgcg ttctatcagg ctcttgtcga gctcggtgga actgcccacc
     7921 agcgtcagtt ctacctgctt gccgagtttt cccgccagat cacgcaccag ccggggatag
     7981 cgactaaaga catattccat cggcatcatg cgaatcgaca tcactgattc ctgcaaatca
     8041 cgggcgttac gttgtaactg ccccatgctg gtaatcaaat caccatgatt aaccgggtcc
     8101 agttcgctgg aacgctgggc aagcatggac tgggtgataa ccagctcgcc gacgaggtta
     8161 attaattgat caaccttttc taccgctaca cggatgctgg tggattcact gctgcgcgtc
     8221 gttttttccc gctccacgcg gccggttggc gcttgttcgg ctgccagttt aagcacaggt
     8281 ggggtagcta tttttggcga aacttctact gtttcaaagg taatctgatc ggcttcaatc
     8341 acaaaacaga gtaccgctgt gatgtcatct tcggcgatat cgcccggtaa tattgccgag
     8401 agagaatccg cccctttcac cacgtcattt aacgttgtca gatgccccag ctcttcttcc
     8461 agcaggtcga cttcgccggc cttcaggcgc gaaaggataa ttcgtcgcgg caactgactg
     8521 cgactttgct catcttgcgg ttcacttttg gcaaccacac ttaatcgggt cactgcggat
     8581 ggcgtttcgc ctttcgcttc taatgccagt tgacgcaagg cctggcagat ataatcgtag
     8641 ctggcggcat ccggctcttg cgactgttta taagcgtcga gctgttcttg catgatgtcc
     8701 ttcgtttcca aaaacagatt gataatgtcg gtgttgagtt gcatctcacc tcgtctggct
     8761 tcatcgagca ggttttccat cagatgcgtg gtttcctgca aaacgctgaa gccaaaagtt
     8821 cctgcccctc ctttgatcga gtgggcagcc cgaaagatgg cattcaattg ttcggcatct
     8881 ggcgcttccg gctgcaaaac cagcagatgc tgctccatat cagccaacag ttcgtccgct
     8941 tcatcaaaaa atgtctgata aaaatcgctt atatcgatgc tcacgctgtc acctcggttc
     9001 ggctgatggc attgtgggaa cactgacctg tggtgcaacc tcaggttttt ctagggcgct
     9061 tactggctca ttctggcttt cggcgttttc atgcaaaatg gcctgttcgg cttgtttgtt
     9121 cagtaccaac aggctgatgc gacggttgac ggcatcatca ggtccgcgat cgcttaagcg
     9181 catcgttgcc gccatgccga cgacacgtaa cactttgccg ctatccaacc ctccgaccat
     9241 cagttcgcgg cgggatgcat tggcccgatc ggcagaaagc tcccagttgc tgtaaccttt
     9301 ctcaccgctg gcgtagggaa aatcatcggt atgacctgaa aggctaatac ggttgggaat
     9361 accgttcagc acaggcgcga tggcgcgcag aatgtcgcgc atatagggtt cgacatcggc
     9421 actgccgatt ctaaacatcg ggcgattctg gctatcgatg atctgaatac gtagaccttc
     9481 ctggaccaga tcgattttga gatggagacg taacgcccgc agtttcggat cggactctat
     9541 caactggtcg agatcacccc gcaatttccg caatcgactt tgctccatgc gttttttcag
     9601 ctcttcgatg ttcggctgct tattcacttc cccctggctt tgggtgtaat catcaccacc
     9661 gccgggaatt gggctttcac tattggaaat gcgatcgccg cccgtaaccg ctgtcgccag
     9721 tggagtccgg aagtactccg caatctgaat caactctttt gggctggaga tggagatcag
     9781 ccacatcacc agaaaaaagg ccatcatcgc agtcataaag tcggcataag caatcttcca
     9841 cgatccatgt gctgccccgt ggcttttggc tttgcgtcgt ttgacgacaa taatcggatg
     9901 cgcttgattc ttcatgcttc ctcggttgtc gtctgttgtt gcggattttt caccgcacgc
     9961 acatgctctt ccagttcaat aaacgacgga cgttcgctgg aatagagcgt tttgcgacca
    10021 aactcaacgg cgataggcgg tgcgtaaccg ttcagattag aaagcagagt gactttgacg
    10081 cactgcatca ttttgctggt ttcggcgctt ttctgacgta aaacactcgc tagtggggaa
    10141 ataaatccgt aagccaataa aataccgagg aaagtcccca ccatcgcatg tgcgataagc
    10201 gcccccagct cggcggcagg acgatcggct gaacctaacg cgtgaacgcc ccccattaca
    10261 gccgcgacaa taccaaacgc cggaagtgag tccccgacca gcgccagact gttcgccggg
    10321 acttctgcct cgctttcgtg cgtctcaatc tcttcatcca tcagggcttc gatttcgaag
    10381 gtgttcatgt gaccgctgat aatcaggcgc agataatcga cgataaaatc aagcatgacg
    10441 ctatccgcga ggatgcgtgg gtagctggcg aagatctcgc tctcacgggg attttcaata
    10501 tcacgttcca gcgaaaacat ccccatctgc cgcgatttcg ccatcaaccg ataaagcaga
    10561 gccagcagat ccatatacat tgctttggtg tatttggagc gacgaaacag caacggcagc
    10621 gctttcagcg tgcctttaat cgctttgcca ttattgccga cgataaacga cccaatccct
    10681 gcaccggcaa taatcaccag ttcagcgggt tgatagagtg ctccaaggct tccaccggtc
    10741 atcaaataac cgccgaaaac tgtaccgaga acaaccaggt aacctaataa gataagcacg
    10801 acatcatcct tccactgttg accatgacag gatgttcagt cgtcaggcgt taacgcgcga
    10861 ttggggaaaa aaaaagcagc ggtacgtcgt taccgctgct ggaatgttgc gccacaccgt
    10921 atcagttaaa cagcctgtac tctctgttca tccagcagtt gtgggataat atcggcagga
    10981 ttctgggaaa gtttacgtct ttttactgcc cgggatggcg gttgacataa gctgcaggca
    11041 aagctgccaa caggctggtg agcgtgggta ataaaattgc cgccgcagca gttgcagctg
    11101 gaaagttgca gtaatccact ttcaacaaac cgcaccaatg tccaggcacg ggttaatgcc
    11161 agcagtggtc cttcttctgc ttgtgggcac tgttcaaggt ataaacggta ggctttgatc
    11221 accgcatcga cgccattaca caaaccggtt ttcagtaaaa actgccatgc attacagaac
    11281 atcgaagcat gaacgttttg ttcccaggtc ataaaccagt cggttgagaa tggcagcatg
    11341 cctttcggcg gtgggcttcc gcgcagttct ttataaagtt ttatcaggcg tccgcgactt
    11401 aactgtgttt cgctttccag catctgcaaa cgagcgccca gggtgatcaa ttccattgcc
    11461 agctgaatat cccgcgcttc ctgaacaat
//
LOCUS       DAJTQQ010000111        11093 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000111 DAJTQQ010000000
VERSION     DAJTQQ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11093
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.111"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..374
                     /locus_tag="OO309_003389"
     CDS             <1..374
                     /locus_tag="OO309_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848410.1"
                     /translation="YDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQ
                     FSGAVVKTREATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQ
                     CLRALHHLRVQSTSKTAPQAK"
     gene            complement(577..930)
                     /locus_tag="OO309_003390"
     CDS             complement(577..930)
                     /locus_tag="OO309_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR5848411.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1068..2714)
                     /gene="groL"
                     /locus_tag="OO309_003391"
     CDS             complement(1068..2714)
                     /gene="groL"
                     /locus_tag="OO309_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR5848412.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(2758..3051)
                     /locus_tag="OO309_003392"
     CDS             complement(2758..3051)
                     /locus_tag="OO309_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR5848413.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3327..4583
                     /gene="yjeH"
                     /locus_tag="OO309_003393"
     CDS             3327..4583
                     /gene="yjeH"
                     /locus_tag="OO309_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR5848414.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(4599..5075)
                     /gene="fxsA"
                     /locus_tag="OO309_003394"
     CDS             complement(4599..5075)
                     /gene="fxsA"
                     /locus_tag="OO309_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR5848415.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            5412..6848
                     /gene="aspA"
                     /locus_tag="OO309_003395"
     CDS             5412..6848
                     /gene="aspA"
                     /locus_tag="OO309_003395"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR5848416.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            6966..8267
                     /gene="dcuA"
                     /locus_tag="OO309_003396"
     CDS             6966..8267
                     /gene="dcuA"
                     /locus_tag="OO309_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR5848417.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8383..8721
                     /gene="cutA"
                     /locus_tag="OO309_003397"
     CDS             8383..8721
                     /gene="cutA"
                     /locus_tag="OO309_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR5848418.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            8697..10394
                     /gene="dsbD"
                     /locus_tag="OO309_003398"
     CDS             8697..10394
                     /gene="dsbD"
                     /locus_tag="OO309_003398"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR5848419.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10431..11006
                     /locus_tag="OO309_003399"
     CDS             10431..11006
                     /locus_tag="OO309_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5848420.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         2620 a         2909 c         2750 g         2814 t
ORIGIN      
        1 tatacgacgt tgactgccag gacaatggca atctggaata tgacacctat tcccaaccgg
       61 aatggaaaca taacttgttc gaccattatc tggcggtgct ttaccgcttt aaagatgaaa
      121 gtggcaaaga acaatttagc ggcgcagtgg tgaaaacgcg tgaagcaacg ccgggtaaag
      181 aaatagaggc gatcactcgc cgaatgcttg atttcagccc acgactgaaa aaactcgcgg
      241 gtgtgccatg tcaggtctat gttcgcaccg tagcggcgaa taacgcccaa ccgctaaccc
      301 aggatcaatg cttacgcgcg ctgcatcact tacgtgtaca atccaccagc aaaactgcac
      361 cacaggcgaa ataatctcat ctttgctaat gcgaggctgg agccatccag cctgaaatga
      421 ctgataacgt gatcgttggt catgccggat gcggcgtaaa cgccttatcc ggcctacaaa
      481 ttctcaagca ttcaatcagt tgcaatgcct gctgtaggcc tgataagcga agcgcatcag
      541 gcaataatac gtttgtcacc cagcctctgg atcacatcag ttcgggcact tataaacctg
      601 accgataatc tggctatccg tcgggacaaa actggacaac attccctgcg acgggctgct
      661 gatgccataa atcacgttac cgcccattgc agccgcctgg ttgcgcagat cgtttgctgc
      721 gccgcgcata gaaccgccct cttctccgtg ttgcccggaa agccagttgc tttgcttacc
      781 tgtcgcagta ccaatcagct ggcactctgc gcctggctgc tcgtccacaa tgcgtacact
      841 ctgaccggca gcactcaatt cgttgctgga gctacaaccc gccatcagta gcgcggcacc
      901 gacaatccct gctaagtatt ttacgtgcat gttattcccc ataatcaatg agctggacgc
      961 cactcgcgtc atccgtgtct gaatcttata ctagaaagat gaccaaaaga aaaaccccca
     1021 gacatttctg cccgggggtt tgtttatttc tgcgaagtgc agggcaatta catcatgccg
     1081 cccatgccac ccatgccgcc cataccgcca gcagcgccta agtcagctgc atcgtttttc
     1141 ggcaggtcgg taaccatgca ttcggtggtg atcatcaggc cagccacaga agctgcgtac
     1201 tgcagagcag aacgggttac tttggttggg tccaggatac ccatgtcgat catgttgccg
     1261 tattcttcgg ttgctgcgtt gtaaccgtag ttgccgtcgc cgcctttaac ggtgttagca
     1321 acaacagacg gttcttcgcc gcagttcagg acgatctgac gcagcggagc ttccattgca
     1381 cgcagtgcaa ctttgatacc cacgttctgg tcttcgttct gaccacgcag gtcagccagt
     1441 ttagacgcta cgcggatcag cgcaacacca ccaccagcaa ccacgccttc ttctaccgca
     1501 gcacgggtcg cgtgcagggc atcttcaacg cgtgcttttt tctctttcat ttcaacttcg
     1561 gtagcagcac ctactttgat aactgcaacg ccgcctgcca gtttcgctac gcgctcctgc
     1621 agtttttcac ggtcgtagtc agaagttgct tcttcaatct gctgacggat ctgagcaaca
     1681 cggccctgga ttgcagcttc ttcacccacg ccatcgatga tagtggtggt gtctttgttg
     1741 atcacaacac gtttagcctg acccaggtct tccagggttg ctttttccag ctccataccg
     1801 atctcttcag agattacggt accgccagtc agggttgcga tatcctgcag catagcttta
     1861 cgacgatcgc cgaagcccgg agctttaacc gcagcaactt tcacgatgcc acgcatggtg
     1921 ttaacaacca gagttgctag cgcttcgcct tcaacatctt cagcgatgat cagcagcggt
     1981 ttgcctgctt tggcaacagc ttccagaacc ggcagcattt cgcggatgtt ggagattttc
     2041 ttgtcagcca gcaggatgaa cgggctttcc agttctactg cgccagtttc cggcttgttg
     2101 atgaagtaag gagacaggta gccacggtcg aactgcatac cttcaaccac gtccagttcg
     2161 tcctgcagac cggtaccgtc ttcaacggtg ataacgcctt ctttaccgac tttgtccatc
     2221 gcttcagcga tcagtttacc tacggtttcg tcggagttag cggagatggt accaacctga
     2281 gcaatcgctt tagaatcaga gcacggtacg gacagcgctt tcagttcttc aactgcagcg
     2341 gtaaccgctt tgtcgatacc acgtttcagg tccatcgggt tcatgcccgc agcaacagct
     2401 ttcagacctt cagtgatgat agcctgagcc agtacggttg cagtggtggt accgtcgcct
     2461 gcagcgtcgt tcgctttgga ggcaacttct ttcaccatct gcgcacccat attttcgaac
     2521 ttgtcttcca gttcgatttc acgagcaacg gaaacaccat ctttggtgat ggtcggtgca
     2581 ccgaaagatt tatccagaac tacgttacgg ccttttggac cgagggtaac tttcactgca
     2641 tctgccagta cgtttacgcc gcgcagcatt ttcacacgag cgtcgttacc gaattttacg
     2701 tctttagctg ccattatctt tattccttaa attcgtatgt tcagtgtcgt gcgcggatta
     2761 cgcttcaaca attgccagaa tgtcgctttc ggacatgatc aacacttctt cattgtcgat
     2821 cttctcggat ttcacaccgt agccatcgtt gaaaataacg atgtcgccaa ctttcacgtc
     2881 cagcggcttc acttcgccat tttcaaggat acggccattg ccgacagcca gcacttcgcc
     2941 gcgggtggat ttagccgctg cagagccggt cagaacgatg ccgccagcag atttagtttc
     3001 aacttcttta cgcttgacga tcacgcgatc atgcaatgga cgaatattca ttgataactc
     3061 tcctttgaga aagtccgtat ctgttatggg tgacgccgga gcttacgtgg tttcccggct
     3121 ggtgaccaga gaaatgggga tgaggcttcg ccccttcaag ggggaaaaag aaaaaaaatt
     3181 ctgatcagca caaaatcggg tgaaaaccct gattcacctc acatttcatc gcaatttctt
     3241 catcgccgtc ataagcgaat ctgattgtgc taccatcgaa aatctacgca gttgcccaaa
     3301 atttgggcgc aatcggatat caacccatga gtggactcaa acaagaactg gggctggccc
     3361 agggcattgg cctgctatcg acgtcattat taggcactgg cgtgtttgcc gttcctgcgt
     3421 tagctgcact ggtagcaggc aataacagcc tgtgggcgtg gcccgttttg attatcttag
     3481 tgttcccgat tgcgattgtg tttgcgattc tgggtcgcca ctatcccagc gcaggcggcg
     3541 tcgcgcactt cgtcggtatg gcgtttggtt cgcggcttga gcgagtcacc ggctggctgt
     3601 ttttatcggt cattcccgtg ggtttgcctg ccgcactaca aattgccgcc ggattcggcc
     3661 aagcaatgtt tggctggcat agctggcaac tgttgttggc agaactcggt acgctggcgt
     3721 tggtgtggta tatcggtact ctcggtgcca gttccagtgc taatctacaa accgttattg
     3781 ccggacttat cgtcgcactg attgtcgcta tctggtgggc gggcgatatc aaacctgcga
     3841 gtatcccctt ccccgcgcca ggaaatatcg aacttaccgg gttatttgct gcgttatcag
     3901 tgatgttctg gtgttttgtc ggtctggaag catttgccca tcttgcctcg gaatttaaaa
     3961 atccagagcg tgattttcct cgtgctttga tgattggtct gctgctggca ggattagtct
     4021 actggggctg tacggtagtc gtcttacact tcgacgccta tggtgaacaa atggcggcgg
     4081 cagcatcgct tccaaaaatt gtagtacagt tgttcggtgt aggagcgtta tggattgcct
     4141 gcgtaattgg ctatctggcc tgctttgcca gtcttaacat ttatatacag agcttcgccc
     4201 gcctggtctg gtcgcaggcg caacataatc ctgaccacta cctggcacgc ctctcttctc
     4261 gccatatccc gaataatgcc ctcaatgcgg tgcttggctg ctgcgtggtg agcacgttgg
     4321 taattcatgc cttagagatc aatctggacg ctcttattat ttatgccaat ggcatcttta
     4381 ttatgattta tctgttatgc atgctggcag gctgtaaatt attgcaaggt cgttatcgac
     4441 tactggcggt ggttggcggg ctgttatgcg ttctgttact ggcaatggtc ggctggaaaa
     4501 gtctctatgc gctgatcatg ctggtggggt tatggctgtt tctgccaaaa cgaaaaacgc
     4561 cggaaaatgg cataaccaca taatccggcg tttcgacatt aatcctggcg atcgtcttta
     4621 tgatcaaggc ggtcgcggtc atcatccttt cgctgatact caccatcaaa agtattaccg
     4681 ccaccggtcc cggcgctaaa accgccgcct ggcatgcgag aaaagcgcaa atgtggcatc
     4741 aacttcactg tcagatgctt ttgcaccggc ggcaataaaa gcagaagacc gaggaagtcg
     4801 gtgaaaaagc ccggtaataa aagcagcaaa ccggcaataa tcagcgaaac acttttaatc
     4861 atctccgccg cagggttttc acccgccgcc attttttgct gcatcagcac gaaattctta
     4921 aagccctggt tacgtaccag tgacataccg ataactgacg tgaatataac cagcacgagg
     4981 gtcagcaata cccccaatac atgggcaacc tgaataaaga ttgaaatctc aatatagaca
     5041 taaaggaaaa tggcaataaa aggtaaccag cgcaaaggtt tctcctgtaa tagcagccgg
     5101 ttaaacccgg ctacctgaat gggttgcgaa tcgccttcag cttatattgt ggtcattagc
     5161 aaaatttcaa gatgtttgcg caactatttt tggtagtaat cccaaagcgg tgatctattt
     5221 cacaaattaa taattaaggg gtaaaaaccg acacttaaag tgatccagat tacggtagaa
     5281 atcctcaagc agcatatgat ctcgggtatt cggtcgatgc aggggataat cgtcggtcga
     5341 aaaacattcg aaaccacata tattctgtgt gtttaaagca aatcattggc agcttgaaaa
     5401 agaaggttca catgtcaaac aacattcgta tcgaagaaga tctgttgggt accagggaag
     5461 ttccagctga tgcctactat ggtgttcaca ctctgagagc gattgaaaac ttctatatca
     5521 gcaacaacaa aatcagtgat attcctgaat ttgttcgcgg tatggtaatg gttaaaaaag
     5581 ccgcagctat ggcaaacaaa gagctgcaaa ccattcctaa aagtgtagcg aatgccatca
     5641 ttgccgcatg tgatgaagtc ctgaacaacg gaaaatgcat ggatcagttc ccggtagacg
     5701 tctaccaggg cggcgcaggt acttccgtaa acatgaacac caacgaagtg ctggccaata
     5761 tcggtctgga actgatgggt caccagaaag gtgaatatca gtacctgaac ccgaacgacc
     5821 atgttaacaa atgtcagtcc actaacgacg cctacccgac cggtttccgt atcgcagttt
     5881 actcttccct gattaagctg gtagatgcga ttaaccaact gcgtgaaggc tttgaacgta
     5941 aagctgtcga attccaggac atcctgaaaa tgggtcgtac ccagctgcag gacgcagtac
     6001 cgatgaccct cggtcaggaa ttccgcgctt tcagcatcct gctgaaagaa gaagtgaaaa
     6061 acatccaacg taccgctgaa ctgctgctgg aagttaacct tggcgcaaca gcaatcggta
     6121 ctggtctgaa cacgccgaaa gagtactctc cgctggcagt gaaaaaactg gctgaagtca
     6181 ctggcttccc atgcgtaccg gctgaagacc tgatcgaagc gacctctgac tgcggcgctt
     6241 atgttatggt tcacggcgcg ctgaaacgcc tggctgtgaa gatgtccaaa atctgtaacg
     6301 acctgcgctt gctctcttct ggtccacgtg ccggcctgaa cgagatcaac ctgccggaac
     6361 tgcaggcggg ctcttccatc atgccagcta aagtaaaccc ggttgttccg gaagtggtta
     6421 accaggtatg cttcaaagtc atcggtaacg acaccactgt taccatggca gcagaagcag
     6481 gtcagctgca gttgaacgtt atggaaccgg tcattggcca ggccatgttc gaatccgttc
     6541 acattctgac caacgcttgc tacaacctgc tggaaaaatg cattaacggc atcactgcta
     6601 acaaagaagt gtgcgaaggt tacgtttaca actctatcgg tatcgttact tacctgaacc
     6661 cgttcatcgg tcaccacaac ggtgacatcg tgggtaaaat ctgtgccgaa accggtaaga
     6721 gtgtacgtga agtcgttctg gaacgcggtc tgttgactga agcggaactt gacgatattt
     6781 tctccgtaca gaatctgatg cacccggctt acaaagcaaa acgctatact gatgaaagcg
     6841 aacagtaatc gtacagggta gtacaaataa aaaaggcacg tcagatgacg tgcctttttt
     6901 cttgtgagca gtaacttaaa aataacaatc taatatcaac ttgttaaaaa acaaggaagg
     6961 ctaatatgct agttgtagag ctcatcatag ttttgctggc gatcttcttg ggcgccagat
     7021 tggggggaat aggtattggt tttgcaggcg gattgggggt gctggttctt gccgctattg
     7081 gcgttaaacc cggtaacatc ccgttcgatg tcatttccat tatcatggcg gttatcgccg
     7141 ctatttctgc catgcaggtt gctggcggtc tggactatct ggttcatcag acagaaaagc
     7201 tgctgcgccg taacccgaaa tacatcacga tcctcgcacc gatcgtgacc tatttcctga
     7261 ctatctttgc tggtactggc aacatctctc tggcgacact gccagttatc gctgaagttg
     7321 cgaaggaaca aggcgttaaa ccttgccgtc cgctctctac tgcagtggta tccgcgcaga
     7381 ttgcgatcac cgcatcgcca atctcagcgg cagtggttta catgtcttcc gtgatggaag
     7441 gtcatggcat cagctacctc catctgctct ccgtggtcat cccgtccacc ctgctggcgg
     7501 ttctggtgat gtccttcctg gtcactatgc tgttcaactc caaactctct gacgatccga
     7561 tttatcgcaa gcgtctggaa gagggcttgg ttgaactgcg cggtgaaaag cagattgaaa
     7621 tcaaatccgg tgcaaaaacg tccgtctggc tgttcctgct gggcgtagtt ggcgtggtta
     7681 tctatgcaat catcaacagc ccaagcatgg gtctggttga aaaaccgctg atgaacacca
     7741 ccaacgcaat cctgatcatc atgctcagcg ttgcaactct gaccaccgtt atctgtaaag
     7801 tcgataccga caacatcctc aactccagca ccttcaaagc aggtatgagc gcctgtattt
     7861 gtatcctggg tgttgcgtgg ctgggcgata ctttcgtttc caacaacatc gactggatca
     7921 aagataccgc tggtgaagtg attcagggtc atccgtggct gctggccgtc atcttcttct
     7981 ttgcttctgc tctgctgtac tctcaggctg caaccgcaaa agcactgatg ccgatggctc
     8041 tggcactgaa cgtttcaccg ctgaccgctg ttgcttcttt cgctgcggtg tctggtctgt
     8101 tcattctgcc gacctacccg acgctggttg ctgcggtaca gatggatgac acgggtacta
     8161 cccgtatcgg taaattcgtc ttcaaccatc cgttcttcat cccgggtact ctgggtgttg
     8221 ccctggccgt ttgcttcggc ttcgtgctgg gtagcttcat gctgtaatga tcaatcgcgg
     8281 ggcgttcacg ccccgctttc tttcccgccg actaacatcc ttcccccgtc cgttgtatag
     8341 tgacctctct cttgcggttc catctgttct tgcgaggtgt ttatgcttga tgaaaaaagt
     8401 tcgaatacca cgtctgtcgt ggtgctatgt accgcaccgg atgaagcgac agcccaggat
     8461 ttagccgcca aagtgctggc ggaaaaactg gcggcctgcg cgaccttgat ccccggcgct
     8521 acctctctct attactggga aggtaagctg gagcaagaat acgaagtgca gatgatttta
     8581 aaaactaccg tatctcacca gcaggcactg ctggaatgcc tgaagtctca tcatccatat
     8641 caaaccccgg aacttctggt tttacctgtt acacacggag acacagatta cctctcatgg
     8701 ctcaacgcat ctttacgctg attctgctac tttgcagcac ttccgttttt gccggattat
     8761 tcgacgcgcc gggacgttca caatttgtcc ccgcggatca agcctttgct tttgattttc
     8821 agcaaaacca acatgacctt aatctgacct ggcagatcaa agacggttac tacctctacc
     8881 gtaaacagat ccgcattacg ccggaacacg cgaaaattgc cgacgagcag ctgccgcaag
     8941 gcgtctggca tgaagatgag ttttacggca aaagcgagat ttaccgcgat cggctgacgc
     9001 ttcccgtaac catcaaccag gcgagtgcgg gagcgacgtt aactgtcacc taccagggct
     9061 gtgctgatgc cggtttctgt tatccgccag aaaccaaaac cgttccgtta agcgaagtgg
     9121 tcgccaacaa cgcagcgtca cagcctgtgt ctgtttcgca gcaagagcag cacaccgcgc
     9181 aattgccctt ttccgcgctc tgggcgttgt tgatcggtat tggtatcgcc tttacgccat
     9241 gcgtgctgcc aatgtaccca ctgatttctg gcatcgtgct gggtggtaaa cagcggctct
     9301 ccactgccag agcattgttg ctgaccttta tttatgtgca ggggatggca ctgacttaca
     9361 cggcgctggg tctggtagtt gccgccgcag ggttacagtt ccaggcggcg ctacagcacc
     9421 catacgtgct cattggcctc gccatcgtct ttaccttgct ggcgatgtca atgtttggct
     9481 tgtttactct acaactcccc tcttcgctgc aaacacgtct cacgttgatg agcaatcgcc
     9541 aacagggcgg ctcacctagc ggtgtgtttg ttatgggggc gattgccgga ctgatctgtt
     9601 caccatgcac caccgcaccg cttagcgcga ttctgctgta tatcgcccaa agcgggaaca
     9661 tgtggccggg cggcggcacg ctttatctct atgcgttggg catgggcctg ccgctgatgc
     9721 taattaccgt ctttggtaac cgcttgctgc cgaaaagcgg cccgtggatg gaacaagtca
     9781 aaaccgcgtt tggttttgtg atcctcgcac tgccggtctt cctgctggag cgagtgattg
     9841 gtgatgtatg gggattacgc ttgtggtcgg cgctgggtgt cgcattcttt ggctgggcct
     9901 ttatcaccag cctacaggct aaacgcggct ggatgcgcgt ggtgcaaata atcctgctgg
     9961 cagcggcatt ggttagcgtg cgcccacttc aggattgggc atttggtgcg acgcataccg
    10021 cgcaaactca aacgcatctc aactttacac aaatcaaaac ggtagatgag ttaaatcagg
    10081 cgctcgttga agccaaaggc aaaccggtga tgttagatct ttatgccgac tggtgcgtcg
    10141 cctgtaaaga gtttgagaaa tacaccttca gcgacccgca ggtgcaaaaa gcgttagcag
    10201 acacggtctt acttcaggcc aacgtcacgg ccaacgacgc acaagatgtg gcgctgttaa
    10261 agcatcttaa tgtccttggc ctaccgacaa ttctcttttt tgacggacaa ggccaggagc
    10321 atccacaagc acgcgtcacg ggctttatgg atgctgaaac cttcagcgca catttgcgcg
    10381 atcgccaacc gtgaacgaca cttgcagtgg gaaagacgga ggaaaatagc gtgcaacgtg
    10441 aagatgtact gggagaagcc ctgaaattat tagaattaca agggattgcc aacaccacgc
    10501 tggagatggt tgctgaacgt gtggattatc cactggacga gctacgccgc ttctggccag
    10561 acaaagaggc aatcctctac gatgcgctgc gttatcttag ccaacagata gatgtctggc
    10621 gtcgtcagct gatgctggac gaaacgcaaa ccgccgaaca aaagctgctg gcacgttatc
    10681 aggcgttatc ggagtgcgtt aaaaacaacc gctatccggg ctgtctattt atcgctgcct
    10741 gtacgtttta tcccgatcct ggccacccta ttcatcaact ggccgatcag caaaaaagcg
    10801 ctgcctacga tttcacccac gaactgttaa ccacgctgga agttgacgat ccggcgatgg
    10861 tagcaaagca gatggaactg gtgctggaag gctgtttaag ccgaatgctg gtgaatcgta
    10921 gccaggcgga tgtcgacact gcacatcggc tggcggaaga tatcctgcgc ttcgcccgct
    10981 gccgccaggg tggtgcattg acctgacaga aacacagaaa agaagcgatt tgccgcaatc
    11041 ttaagcagtt gaatcgcttt tactgaaatt aggttgacga gatgtgcaga tta
//
LOCUS       DAJTQQ010000112        11079 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000112 DAJTQQ010000000
VERSION     DAJTQQ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.112"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..270)
                     /locus_tag="OO309_003400"
     CDS             complement(<1..270)
                     /locus_tag="OO309_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5848421.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNNDVWC"
     gene            complement(364..926)
                     /locus_tag="OO309_003401"
                     /pseudo
     CDS             complement(364..926)
                     /locus_tag="OO309_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1130..1336)
                     /locus_tag="OO309_003402"
     CDS             complement(1130..1336)
                     /locus_tag="OO309_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR5848422.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1443..2006)
                     /locus_tag="OO309_003403"
     CDS             complement(1443..2006)
                     /locus_tag="OO309_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5848423.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3381..5216)
                     /locus_tag="OO309_003404"
     CDS             complement(3381..5216)
                     /locus_tag="OO309_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848424.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5317..5604
                     /locus_tag="OO309_003405"
     CDS             5317..5604
                     /locus_tag="OO309_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5848425.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5576..7105
                     /locus_tag="OO309_003406"
     CDS             5576..7105
                     /locus_tag="OO309_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCR5848426.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7275..8492)
                     /locus_tag="OO309_003407"
     CDS             complement(7275..8492)
                     /locus_tag="OO309_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5848427.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8859..9845)
                     /locus_tag="OO309_003408"
     CDS             complement(8859..9845)
                     /locus_tag="OO309_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848428.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9842..10370)
                     /locus_tag="OO309_003409"
                     /pseudo
     CDS             complement(9842..10370)
                     /locus_tag="OO309_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
     gene            10437..11079
                     /locus_tag="OO309_003410"
                     /pseudo
     CDS             10437..11079
                     /locus_tag="OO309_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT         3417 a         2570 c         2137 g         2955 t
ORIGIN      
        1 acaccagaca tcgttattaa gtagcagcac cataagataa ggagtggcat cgttagccac
       61 agcttcccta ctccagaggt aatataaagg ggtgggctcg actgatttat ctttacgtcg
      121 cttacactgc aaatgttcag aaattagtct atgcagctca ccagtaaaat cagcaatcag
      181 agaaggaatt gccttattca taccagggca aggtattaat ttaaactgta ataatttaat
      241 tttaggatgt gtagcagcag cccgatacag agttgcaagg acacactttt gccagagggc
      301 gttacaggaa agcttaatgt ttgattctgt atacataata aatcacctta tagttacaat
      361 aggtcaaaaa ccgctgtagc caaagttacg ctggcctgat gctttagtgt cgagcttcgt
      421 caaataatcc agacgtacca ataagcgctg gtactgctct gggaaattag gatcgttaat
      481 atccaggatg taacgtccat tagcagggaa atgaactaat ccctggtgat cttccagttc
      541 caaccctaag gcactgtacc aggctccggt aattaaccct ctcagggtgt cagagcgctc
      601 atagtttccc agatggtaat aggcgtcttt gttaaaaaaa ggcagacgtg ataatgacat
      661 ttaaaggact gcgtatattc tttcgcccag ataatacaaa ccgacgttgc gtaggtgcgt
      721 ttgccttgtt gttttttacg gtgttgatct gcattaattt tcgcattcag cgagttacgg
      781 aaacgagata tctctccggg gatcaggtta gggaaacagg taatgatatc cccgttgtca
      841 atcacaggag ggtaatggag atccccccgt attgctgtta ttctggaagt gtttttaata
      901 ttcgtattga tagttctcag agatatatca cactctagat attttaatta agtatcagtt
      961 cgatatcggc atattgtaca ctacctcagg tagatgatga gatcagatac agggatcaac
     1021 tgacggtgag cgcagtcccc acattttatc cagggcttac tgcatattcc caacagccat
     1081 tcgttagcca ggtagacgaa taccctgatt tgccgctggt tttgctttcc tatcgaaggg
     1141 cccatacatc atcacgccca cgaaacaacc gacgaaataa cgcaactttc tcatccgtgg
     1201 ataatacgaa aacgcactgc acagggctct gcggtttacg tcgccattca atcccatgag
     1261 cttcaagtaa tgagaccagc cgggtatttt ctgcccgcag tactgctagt tcatcgctgt
     1321 cagatttata aaccatatgc gttatcactt gtacctgtct cccggcggca ccacgactga
     1381 aattcgtgtg aaggcaaatc cacgcgataa cttacgatag ttcggacata agcccccata
     1441 attcagttat gtcgtggtcg accccggtta cttttttcgg gaagcctgct acggcccaga
     1501 ataataccgt gttctttctg atcctgttta gccagatagc taagggcgta acgtaagtcg
     1561 tctaccgcag atttatcact atagtgaatc acgtgatcga tacgcaccgg gtatttgtct
     1621 ttagcccggc acagatgaaa ataaccctcc ccttccgtga tccgcctcca gatatctccc
     1681 agttgcctgg atatctgata agagttcttg tgacgttgtc catccaggta gccgataaaa
     1741 tgaatatgaa gccctttgtc tggtgtatat tccattaccc agtaatatcc cgccaacatc
     1801 gtctgggttt cgctaagtaa acggtatatt tccatacaca tactgtgctt acaggaatgc
     1861 ccgaaactgg gcgtgtcttt cctgtaagca aaatcaattc tgaaaggtaa cagtttagaa
     1921 aaacgttgaa acataccatc catgtgttca ttcacgtctt tcagaatcat gaaatccatt
     1981 ttgtaattgg gattggcatt atacatttta tagtccttac tttataaaac tcacaggaag
     2041 agagaattac gtaaatactg agagtagaga aacgccgtac cacggcatta ataaacgaag
     2101 taaaccagca gcacagacaa cctgataaaa ctgttttgta ttaccgcatt actgtgatga
     2161 ggtaatactt cagaatacag tgagactctc ttaaactgag aaccagtacg aattacaatg
     2221 gaaaaacaca cctcatagaa ttgagtttca ggaagcacca ctaaattctt tacaggaagt
     2281 aatctgacat tcacaaaact cagtatcggc tagctacagg caacatcgct attaatattg
     2341 tcgaaccatt aagtaaagcc gagagagacc acgggtattt aatcattagc ataaaagaat
     2401 aatggtatac agaacatacc cacacaccat aagaagattg ttccatacag aaaagatcat
     2461 atttacctat acctataaac tgtaacggag tcataacacg ctctaatagt gttccgatac
     2521 gtaaccagtg ttaagtacgt acaggacaac attacacaca taattcacca tacagcttcc
     2581 cccatatgct tcactgcgta gtgtatggag atatatgcat tacacacata cagaatactt
     2641 agcataaaga ggagtgacat taccacgtaa agagataagt tatacaacaa acatacaaac
     2701 aatacggcac agacacacaa taccataact ccaactaata atactaatta accttaatta
     2761 tttatggtat atgaaataaa tgtgattctc ccggaactaa ctaacctaac cagacagatg
     2821 atcactgtat aaaaacttca gttaatactc atttgttttg tcgactcagt atataatgag
     2881 tgaccagtaa tcaggataat cacacttaca taacctgtaa aaaagaacaa gaagaataag
     2941 ccactaacat aatgcaaaat ggcatctgaa tcgatcgcag tttggcattc gaatcgatca
     3001 tgatttggcc caatccgtga tcgaatagag cggaatacgc aacataattc accagacaag
     3061 agcttacacc gaattacaga ttatgtatta atgtcaaaac tggcatattt tacagataca
     3121 atgaacatgc aacccatcaa tctaataggg gcggtatgta catctgccca tgaatgggat
     3181 ggcgctaaga atacaaaaca attagacata gatcacaaaa acacaccatt ctaattgaaa
     3241 aacaaaacaa acaaataaat catactatta tagaattttc tcttgccaga catagataat
     3301 acccctgcta attttgctaa tggttagcaa aattagcagg gtatataaag attattgacc
     3361 caaaacacac tcagatgaat tcacgacagc tcaggagggt tattcctgat gtcatcttca
     3421 caaataatgg ttaacgcatc atatatcatt ctgcgtctgt tcctgttttc ggccttatcg
     3481 ccatacaaca tcatcacatt tttaatttca tcgaccggtg atttcacata gtgaactaat
     3541 acaccaagct tctcaacaca ttgcagtaaa gtatatttct tactgcctgt gaaaagccct
     3601 atcatagttt ctttacgttc gcgactggat aatactttgt tttccataat gacttctgcc
     3661 agcgtcttac catcataaac atactcttta tctatcagaa tatttatctt atccagcacc
     3721 ttctctacgt catcaagcaa acctgtaata tatgggggcg caatatatgg tttcgtactg
     3781 atcttttcaa gcatacaatt atacatctta ataacacgca tcagggtaaa attgttcgca
     3841 tcatactgta tttcaccctc cttatataca ggatcaataa gtggtatttt ttcaataaat
     3901 gcatcctgat aggcttttac atggtcaagc caataaaaca acgagccata ctttatattt
     3961 tttctattta ctttaacctc cagtgctgtc cgaagcaacg ataatgcccc cctgacatat
     4021 cctgaaggta attcaccaaa caatcctttt ctttccatgt catcataatg ctgcagcaca
     4081 tcattaattt tatttttcct tatcagacaa atgtacaccg caagtgtttc gtagcagctg
     4141 gtcacatgaa cagcctcatt ctgcaactgc ccaaaatagc ttataatctt tttcagatat
     4201 gcgtcatcac aaccattctt atcattaata ttaaatacaa gatctttcag acgggaaata
     4261 tattcctgag atttatcatc aagtgaaaca ctataacgag acaatgttcc ttctgcactg
     4321 ttttgaaact tataaaacat ttccagtaat ttttttgcat aaggcgttgt atcaaagctg
     4381 gtaaagtcat aaagatattg cgcagcgtaa ccatacaaac catgaatcca gagtccttct
     4441 ctcaccaaca ctctgagata atatgcaagg ctttgaagta tcttcgtctt tcgttgtata
     4501 tcaagtaaga cacttaatga ataaaatgca ttatagaatt tatcaatatt cctgagcact
     4561 ttctccagtt catccttatc cagcttcttc tcccggtaat cctttttata ttcatttttg
     4621 atagccatga tatattgttg catcaggctc ttgcttttcc cgttatcctt ccgtatagtt
     4681 cctgtatatt tccccagagc atacagcttt ccattttcat gaaaatcttc cttgttgaat
     4741 cccagttcag ccagatattc ccgaagataa cgtttaacag cagcttcatc gctttctgta
     4801 tcagcgtgat caagacaagg acaatacgat ttctgtaaaa aattataaat atgataataa
     4861 aaagcagagt cattctctgg tctgtatgta ttatacatat ctacaacaag aaaaataacc
     4921 ccaaaagtgc gcagacaata gctgcgaagg aattcaggtt ttagtgtgga atgcactgga
     4981 taagaattca tcatgtaata aaaatgttca tatcgggcat atatgatccg ccgttgctcc
     5041 tgactgaaca ccccttcagc atcaataata cgactgatct cattataacg ttgatcccag
     5101 aatttttgat gcatttcaaa attggtactc ttccgcttgt gtgccggatt ctcgccttcc
     5161 ttaaacaacc ccaaaacagt acaaatatca tacaacatat ttgtaaatgt gcgcatatgc
     5221 tgtgctctcc ggttggtttg cattttatcg cagaggacat tctgagctta acgtatcaaa
     5281 ctgacacaac gacatgcaat gcatatggag aacaatatgt caaatcaaac cggacagctt
     5341 tacacgcctt cacgcatcct gcgtatggct gaacttaccc gactgctggg tatatcaaga
     5401 tctactattt acgtgaaaat aaatccggcc tcgaaatacc acgaccctac ctttcccaaa
     5461 ccaataaggc ttggtagtgc atccgtaggc tggcgggcat ctgccatcga tgagtggctg
     5521 atgttacata cggcaccagc ctggtctgaa cctgaaaaca aactaaagga acttaatgat
     5581 gacgacaaaa ctatcactcc ctgaacttga cacaactaaa ctgcctgaca ggtgccaggc
     5641 attacttgat gaaatgcatg aagaaacagg aatacgccgt gaaatacttc tgtctgttat
     5701 gctgacggtc atggctgcct cggtccagga tacgcatgaa gttgaacttt ccggagggca
     5761 acgtaccagc cttcagatat ttatgtgtct ttcatcagca tcaggcagtg gaaaaacctc
     5821 tgcctgcgca aaattaatcg ctcctgttca tgaaacagaa gaagaactac atcaggcata
     5881 tatcgacgat aaaaaaaact atgaccgaat gatggaaatg tggacaacag ataaaaaaat
     5941 cctggagcgg agatataaaa aggaaattga aagatccccg gaaaatgctg ctgctgcacg
     6001 agcagcactt gaaaagtgta tagcaaataa acccgaacct cccgtacaac aggttctcat
     6061 cgtgaatgac gcgacaccag aagggatagc tttgaaactc agccagtcac cctctcttct
     6121 gttgctgtct gatgaaggag gtactattct ggataagcgt ttcgaacgca aatcggcact
     6181 gtacaacaca ttatggagtg ggcaaccagt caacgttgaa cgggcatcca gacccgggtt
     6241 ccggctgaat gatgtccgtc tcactatact tatactgact caaccggtaa tatttaataa
     6301 gttttttact ctcactggcg atcagatccg gggcaatggc tttctggccc ggttgctatt
     6361 ttgcgaaccc ggagcaatta aaataatgac gacagaaccg catactgcca ctcctgttgt
     6421 gacgcaacaa tgtgcaagct gttttcgtaa gaaaagtttt ggcagtcaga tcagagactc
     6481 tctgagagca tccagagaaa gacgtgcaaa aagtaaagag cgcatctgca tgacattatc
     6541 aaatgcagca tcccgcgcac tggagcgctt tcacaaagaa aatatggata ctgtcagaca
     6601 gaatcctcat atgggtacat ttgaagacat tattgtcaga aaaagagagc aggttgtcag
     6661 gatagcagca cttctggaac tggagaaaga tccagactcg acagtaataa cgctggaaag
     6721 tactaatagc gccatttatc tcattgaatt ttatttcaag catcttatat ataaacttga
     6781 atcccttcgg gaaatatccc cggctgagaa acttgataaa tggcttcaga aaagaattat
     6841 cacaacagca ggttacatat tccagaaaag ttatatttta caatatgccc catatgctct
     6901 caggaaaaaa tgtgttctgg atgaagcact tgacatactg gcggaacaaa ggaaaataag
     6961 gattgatgat aacctggttg tttatatagg aaacacgatt acaccatccg aactggcaaa
     7021 aaaactaaat attcctgcat tcgatgccgg agttttcatt tgtgatcatc aaaacatact
     7081 gaagtatcac cgaaacagac tatagatact atcggacaaa aaataaatta tcacaagtgt
     7141 attattcgtt ataataatga ataatcgtta tctgagtgca atgccaggtt ttcattacga
     7201 actggcattg catttatttt tcaaaaaaga aagcagtaac catttggcta ctctgagtat
     7261 gccagtccat gaaactaagc gaattcctta gtgtttttag caagttgaga catcacataa
     7321 tccgcccatg cctgcatcat cggtacacgc tgctccagta gatcagtacg atgatatgcc
     7381 gcctcaacct tatttttcag cgtatgagcg agcgcccttt ccgccagatc ccgcgaatac
     7441 ccctgttcac tacaccagtc cctgaatgtt gagcgaaaac catgtgccgt ggcaactcgc
     7501 cccggaatgt cactgacggc tttcttttta cgcagaaaac ttgtcaacac catatcggaa
     7561 aggatctgct gctttctggg tgaagggaac accagttcat catgcaggcc acgtatattt
     7621 tccagaatgt aaatagcctg ccgggataaa ggaacacgat gctgtagcct agctttcatt
     7681 ctttctgcag gtatagtcca tacccgctta tgaaaatcaa tttcagccca gcgcattccc
     7741 ctggcttcgc ccgagcgagt tgctgtaagt atcaccatta ataacagtgc gcgggtaaca
     7801 ttataaggtt catcactata cacactggtc gccacaaaaa gcggtaactg cctccagggc
     7861 attgcgggtt ggtgttcatc acgtcctctt gtctgctgag gaagcaaatg gtcaaccaca
     7921 tcaacaggat ttgccacaca aaaaccgtgc gcccatcccc actgcataac aacatgaatg
     7981 cgctgtttaa cccggcttgc cgtttctgac aaggttaacc agactggacg cagtgtttct
     8041 gccacgtccg cagccgtaat cgaatccagc gtttttgctc ccagttgagg aaacgcgtaa
     8101 ttctcaagcg tcgataacca ctgccttaca tgctttggat tctcccatcc aggagacagt
     8161 tctgcatgta cacgcctggc tgcatcggca aatgttggga taacgacttt ctcagattca
     8221 gcctttttaa tctccagagg atcatcacct gcagcaagtt gctctcgcat tatccgggca
     8281 gtacgtgcag cttcagcaat actgacctct gggtaagttc ccaatccagc attacgtctt
     8341 ttttgtgtca ccggacttac ataacgaaaa acccatttcc cccgcccctt tactgaagaa
     8401 ggatgaaggg tcagtccggt aattccccca tggggcaatg gtttgtcatc aggtttgata
     8461 tgtcttgctt tcgtatccgt caatactgcc atacgctaat tcccgtcact ctggtatgcc
     8521 atccagtatg ccattagtcc ctcgcttcac taagaataca ttagataaca tcggacaaca
     8581 aaaagtgtaa tacattgttt ataaagaaat tatataacac ccctggatta agtcagattt
     8641 atttcaggcg gtccccctca ccgccatatt taaagaagag cgcgtacgaa agtacgggct
     8701 tttttttcgt atattgtaca caccgggtgc ccccgcccac gtctgttagg gcgaggggaa
     8761 attgtgcaca tcaactgtct ggttactcag tcaacaatca aaatgcgtaa gatagtacag
     8821 aacatatatg cgttatctgg tcacttcctg taacttcctc acttattaaa atgcccccaa
     8881 cggaaataca tgtcacatgc catcataaaa tcgttatgcc agaggaatcg acaatggaaa
     8941 aaatagtaaa ttaagcacgg tacatagcag agtaaaaaat aggcttcaaa ccaaatccgc
     9001 cattccacaa gcgttgccaa taccgacacc agcgtaatcc acatcatttc aataacccgc
     9061 aatatgaata accgaagcca aagcggcttg gcaaaaaagg tcgacaacgt attttcactg
     9121 gagaaaaaca gatcgatttc ctgcaaaaaa aacctgccca gaaaagcggg ccgcttggag
     9181 aagcgcagaa aaaacagatt aatcgccaac actacgccac aaagaatgag caatgcaatt
     9241 atcgatttaa acgttaacca ctgcccctct gtcacaaaag aatcactgtt acgcagcggg
     9301 ctgatagtaa gcaggttaac aaccgttgag atgcacaccg aaagcagcac atagcgagtc
     9361 agacttttat attttacttt tttcgctact gaaatagcga cctgcttgac ataactggtg
     9421 cggatcatcc gaaacagcag aaagatgacc aacggcccaa gtaacgaaat caggaacagc
     9481 gcatataacg agggtagaag ctggtatagc ccccaactca ttgcactcaa catgattaac
     9541 gacagaaatc ctgccaccag aggttgtgta agaaataatt ctattttgtg gttctgcttt
     9601 ttcttaaacc acgaaaaatc gatattcttc gtgacgcatt tataagccca gtacgccttg
     9661 agatcaaaaa taaaattata cagcaccagg gccagaatcc cactaactac accaatatcc
     9721 tctgatggaa aagtaaaccc tgcatttttc caataaaagc ctatcagaat aagtccaata
     9781 gcataaatgt atatcagatt tcgtttatcc tgaaagcgaa taaaagttaa ataatcagga
     9841 atcatttgaa tacctttgca tcactaaatg ctttacgaca atgcataact cgttgctgat
     9901 ccacgccagc aaaatcattt aacatattcc ccaaataggg cttattactt atttcaagga
     9961 aagctaccgg ggttggcata ttcagaatat gtatcgagag attcagtcca tttcaccgta
    10021 tgagttaagt gtaatgagag attttctttg ataattgacg gagcgacctc gctttttgct
    10081 gttacgttca tcagaacctg atgctctggc gaagcgatat ccagtccggc aagataatcg
    10141 cgcattgcct gaacgccgtc ttccatcaaa cgcgtgtgcc aggcaccgct cacaccgagt
    10201 ttaaccggtt cgtatccagc ggccatcagc agcgtggtaa attcattcaa cgcagcctgc
    10261 gttcccccaa taacctgctg gcgcggcgtg ttatcacagc taatatccag cgcaatgcct
    10321 gattccgtaa tcatcgtctg cagttgttcg cggttgatgc ctttaaccgc ctgatcttac
    10381 ccagcaatag tggacacgcg gctaagtgag taaactctca gtcagaggtg acttacatga
    10441 caaaaacagt atcaaccagt aaaaaacccc gtaaacagca ttcgcctgaa tttcgcagtg
    10501 aagccctgaa gcttgctgaa cgcatcggtg ttactgccgc agcccgtgaa ctcagcctgt
    10561 atgaatcaca gctctacaac tggcgcagta aacagcaaaa tcagcagacg tcttctgaac
    10621 gtgaactgga gatgtctacc gagattgcac gtctcaaacg ccagctggca gaacgggatg
    10681 aagagctggc tatcctccaa aaggccgcga catacttcgc gaagcgcctg aaatgaagta
    10741 tgtctttatt gaaaaacatc aggctgagtt cagcatcaaa gcaatgtgcc gcgtgctccg
    10801 ggtggcccgc agcggctggt atacgtggtg tcagcggcgg acaaggataa gcacgcgtca
    10861 gcagttccgc caacactgcg acagcgttgt cctcgcggct tttacccggt caaaacagcg
    10921 ttacggtgcc ccacgcctga cggatgaact gcgtgctcag ggttacccct ttaacgtaaa
    10981 aaccgtggcg gcaagcctgc gccgtcaggg actgagggca aaggcctccc ggaagttcag
    11041 cccggtcagc taccgcgcac acggcctgcc tgtgtcaga
//
LOCUS       DAJTQQ010000113        11021 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000113 DAJTQQ010000000
VERSION     DAJTQQ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11021
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.113"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            383..1213
                     /locus_tag="OO309_003411"
     CDS             383..1213
                     /locus_tag="OO309_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5848429.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1354..2127)
                     /gene="uxuR"
                     /locus_tag="OO309_003412"
     CDS             complement(1354..2127)
                     /gene="uxuR"
                     /locus_tag="OO309_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR5848430.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2342..3802)
                     /locus_tag="OO309_003413"
     CDS             complement(2342..3802)
                     /locus_tag="OO309_003413"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR5848431.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3883..5067)
                     /gene="uxuA"
                     /locus_tag="OO309_003414"
     CDS             complement(3883..5067)
                     /gene="uxuA"
                     /locus_tag="OO309_003414"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR5848432.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     IR"
     gene            5407..6750
                     /gene="gntP"
                     /locus_tag="OO309_003415"
     CDS             5407..6750
                     /gene="gntP"
                     /locus_tag="OO309_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR5848433.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7001..7903)
                     /gene="fimH"
                     /locus_tag="OO309_003416"
     CDS             complement(7001..7903)
                     /gene="fimH"
                     /locus_tag="OO309_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR5848434.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7923..8426)
                     /gene="fimG"
                     /locus_tag="OO309_003417"
     CDS             complement(7923..8426)
                     /gene="fimG"
                     /locus_tag="OO309_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR5848435.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8439..8969)
                     /gene="fimF"
                     /locus_tag="OO309_003418"
     CDS             complement(8439..8969)
                     /gene="fimF"
                     /locus_tag="OO309_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR5848436.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8979..>11021)
                     /gene="fimD"
                     /locus_tag="OO309_003419"
     CDS             complement(8979..>11021)
                     /gene="fimD"
                     /locus_tag="OO309_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR5848437.1"
                     /translation="RIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNK
                     WQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPV
                     IHGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIF
                     TVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQL
                     ADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNI
                     QLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLT
                     VTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGR
                     DQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLS
                     YSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHAN
                     GVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNT
                     LADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQS
                     SGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2676 a         2821 c         2678 g         2846 t
ORIGIN      
        1 tgctacttaa ttccggtggg aaattattta tttccgcata atcagctatg tattatttga
       61 aacaattagt ttgtaaataa tataaattga taacatatca aatacatcaa aaataccatt
      121 cacaggcatt attaatatag acctcaatat agcaatatca aattccaatt agtaataaga
      181 tatgctttta aattgcatgc aaataatttt aaatgagcgt tttttgatac tttagagtgt
      241 ttcctaccct ttaatatcac tgtgtagagt tgatatcact acaacagaaa catgcatgtc
      301 atttgtttac ttctcttttt actacagaaa aatttatcta tttttctaaa catcagttaa
      361 atactcataa aaggtaacta ttatgtctat gccattaagc aatgccgcta ataaccaatt
      421 caatacaaat aatcacttcc ttcatcatcc aaaagtcgac agtgagttaa cacgtcgata
      481 tgaatatgca cgcctggata cggaaaatat ctatcttctg cctcttgctc gtggcaataa
      541 ccataattac gatggaaaaa gcgttgtcga aattagaaaa cttaatattg gcaaacagtc
      601 ctggccattt aattatgtga cagatacatg cagagaattc aatggtatta caacaacagg
      661 acgtatgctc tatcgaaact tgaaaatcac ctcagcttta gacgaaatat atggtggtat
      721 atgtaaaaag gcgcatgctg ctacagagtt agcagaaggt ttacggctaa atttatttat
      781 gaagagccct ttcgatcctg ttgaggacta tacagttcat gaaataacat taggccctgg
      841 ttgcaatgtt cctggttatg ccggaaccac gataggatat atttcaaccc ttccagcttc
      901 tcaggcaaaa agatggacca atgagcagcc aagaattgat atctatattg atcagataat
      961 aactgtctca ggggtagcaa attcttccgg attcgccctt gccgcattac ttaatgctaa
     1021 tattgagatg ggaaatgacc caatcattgg tatagaagcc tatcctggta ccgcggagat
     1081 ccattcaaaa atgggatata acgttatccc cggtgatgaa gatgcgccac tcaaacgcat
     1141 gactctgcaa ccgtcatctc ttccagaact gtttgaatta aaaaatggtg aatggaatta
     1201 tattggtaaa tagccactga gaagaatgag tactaatatt caggttatac agatatttaa
     1261 acaaaaccct gcaatttaca aagtaatact cattgcgact tcttaattta agaaagcgca
     1321 aggaacgttt acccttgcgc ttattataat aagtcaggcg tcgactttat ccagcggcca
     1381 tgaatcaaag agatagccgt caaaataaat atcgtcaacg ttcgagaatt ccagcagacg
     1441 ttgcttaacg ttttccagat gctgccacat tgccagcttc gccgctcggg catctttttt
     1501 gattaacgcg gcgaggatct gtttgtgatc gcccaaccac tctttgcgat acaggctgtc
     1561 atccagatgg ctgtgcaact ggatccacat tggattgttt tcccgccact gccaggactg
     1621 acggaataac tccaccagca tgctgttatg cgttgcttca gcaatggcga gatggaactg
     1681 catgtcaccg ctttcgctgc tacccggcgc gctggaagcc agttcacgct cttccagttg
     1741 caatgcctga cgcattttga cgatatcttc gcgggtagcc tgcagtgcgg caaactcggc
     1801 gatgttactt tccagtaact gacgcgcctg taacagctca aaaggacccg catcattgca
     1861 gacgttggca tccggactgt ctgtgttgtg gctgcctgag ctatcaagca catagatacc
     1921 ggcaccccgg cgcacttcca ccagcccttt gatctccagc atgatcagcg cttcacgtac
     1981 caccgtccgc gtgacatcaa gcatttctgc aatttcacgc tccggcggca gccgttcgcc
     2041 aggattgtac ggcgtcttta tgatcagatc gtggatcatc gccccgactt cctggtaagg
     2101 tctttgcgca gaggtgacag atttcatggt gttgtgtccg ttaggtgcgg ttaatcaaat
     2161 ttttgccgga tgcggcgtga acgccttatc cggcctacaa cgggcaattt tacggccccc
     2221 gttaatctca aactatacca ttagcactgg aagttggcac gtacaggtgg gcaaaaaaac
     2281 gaaaactggt caaccaaata tcagtttgat tgaccagtaa gtctgtaggg taatcagtta
     2341 gttacagcgc agccacacac tcgcgcgcac cgcgttcgca cagctgctga tatgccgacg
     2401 tcaccgcgcc aacaaagtcg gcattctgcg gcagatcatc ggcaaaaata ccgctcaggc
     2461 ccagcagcgc tttcacgcgg tctgcgccct gatactgcgc gttaatcttc tggaactcgg
     2521 ccagcatcgg gtcgaccacg tcaatggcat taccctgctc atccacgccc tgggtgtaac
     2581 ccatccagcc agccacgccc agcgccaggt gacgccagct gccgccgttt tgcaggtgca
     2641 gacgcaccgg gtccagtaga cgctgcggta acttctggct gccgtccatc gcaatctgcc
     2701 aggtacggtg acgcagagac gggttgctga aacgctcgat cagcagcgtc gcataggcgt
     2761 tcaggtccgt accttccggc atcgacagcg ttggcgcttg ttcctgcatc atcagggcaa
     2821 aggccgcttt gcgataatcc gggttagtca tggtgtcggc aatggtttca tagccgccga
     2881 ggtaaccgag gtacgccagg aaagagtggc tgccgttcag catacgcagc ttcatcattt
     2941 cgaacggcac aacgtctgca acgaactgtg tgcccacttt atcccagtcc gggcgaccat
     3001 taacgaagtt atcttcaatc acccactgac ggaacggttc gcaggcaatg gcgcacgggt
     3061 cgtaaacacc cagctggtca gcaatttcct gtaaggtttc tggcgtcgcc gccggaacga
     3121 tgcggtcaac catggtgcac gggaaggtga cgttctcttc aatccatgcc gctagctgcg
     3181 ggtcacgcgc ctgagccagc cccagtaccg cgaccttcgc cacatggccg ttttcacgca
     3241 cgttatcgca ggacatcacc gtaaatgctt tcagcccttt ttcacgacgc agacgcaagg
     3301 cttcgacgat gtaaccaatc gcggacttcg gcgcagtcgg gttttccaga tcgtgcttga
     3361 tcagcgggtt attgagatcc agttgaccgc ttgccgcatc agcgcagtag cctttttccg
     3421 tgaccgttag agagacaatc gccgtttgcg gacgcgccat cgcgttgaga ataccttcgc
     3481 agccgtcgat ttccggatgc agcgcttctt tcatcgaacc gataattttc agctcggtgc
     3541 tttctgcgcc tttttccgct acggtgtaca gcagttgctg ttttttcagg ttttcgatca
     3601 gcatgcggtc gttgcctggc atcaggttaa cttcgcagat gccccagtcg ctgtcggtgc
     3661 tttccagcag atgatgggta tacagcgcct ggtgcgcgcg gtgaaacgcc ccgcaaccga
     3721 gatgcacaat gcgtgattcc agacgagaat gatcccatga cgggcgggca accggcagat
     3781 tgctgtcaac aatagtagtc atcgtaaact ccagaatccg tcttcagaga gtgacggcaa
     3841 ggaagaagga accggcacgg cgcgcagcca tgccggtggg tattaacgga taaagaaagc
     3901 gcgctggatc gccagttcga caccgcgaac ttcggccagg cctttcagac gaccaattgc
     3961 ggagtaacca gggttggttt tcttcttcag gtcgtccagc atctgatgac cgtggtccgg
     4021 acgcatcggg atcaggtctt ctttgccttc cgctttacga cggtgttctt cttcaacaat
     4081 cgctttcacc actttgtaca tatcaacgtc accgttcagg tgcgccgctt cgtggaaggt
     4141 tttcgggtta tcttcacgca tggtggagcg cagatgggtg aagtaaatac gcggaccgaa
     4201 ctgcttgatc atatcaacca gatcgttgtc agcacgcacg ccgtaggaac cggtgcacat
     4261 ggtgaaaccg ttcgccatgc tgtttacggt atcaaccatc cactgcatat cttcaatggt
     4321 ggaaacaatg cgcggcaagc cgaggatcgg gcgcggcgga tcgtccgggt gaaccgccat
     4381 acgcacgcca acttcttcag caactggaat aatcgctttc aggaagacgg caaagttttc
     4441 gcgcagtttg gctttgtcga tatctttgta cagctccagg tgcttacgga actggtcgag
     4501 ggtataccct tcttccgcgc ccggcagacc ggcaatgatg ttacgcgtca gacgcgcttt
     4561 gtcttcgtcg ctcatagtgg cgaagcgttc agctgcctga gcaatttctt cttcggtgta
     4621 atccgcttcc gcgcctggac gtttcaggat atgcatttcg aatgcagcga attcgatctg
     4681 gtcgaagcgc agagctttgg agccgtctgg cagcacgtat tcgaggtcag tacgggtcca
     4741 gtcgagcacc ggcatgaagt tgtagcacac ggtgcgaatg ccacactgcg ccaggttgcg
     4801 cagagtctgc tgatagttag cgatccactg ctcatagttg ccagtgtggg ttttgatatc
     4861 ttcgtgaatt ggtacgcttt ctacgacaga ccacaccagg cctgcgtctt caacgatcgc
     4921 cttgcgtttg aggatctctt ctacggacca tacttcgccg ttcgggatat ggtgcagcgc
     4981 ggtaaccacg ccagttgcgc ccgcctgacg gacatcagct aaagaaaccg gatcgtttgg
     5041 gccgtaccag cgccaggtct gttccatgat ttttcctctt tcacatagtg ggttccacag
     5101 caaagagacg cggaaccttg gagttgttta ggtatccgga tgaacaacca gaccgcagcc
     5161 gtggcgttgc atcggatgag atggcgtata agttctacct tttggtaaga catgtcaacc
     5221 agaattctga aattggttaa ccgcgtcgca agaatttcac tctcatggtt gttgctgcaa
     5281 attgcagcaa agggtaatcg atggttattg atcagaggat gtgacattca tcgcaacaat
     5341 ggttgaccaa tttacataac atgtcgccca aaataacaac ggttcaacca agtaaggtaa
     5401 aacgttatgc atgtgcttaa cattctctgg gtggtattcg gcattggcct gatgctgata
     5461 ctgaatttga agttcaaaat caattcaatg gtggctttgt tggtggcggc gctgtccgtc
     5521 gggatgctgg caggcatgga tttgatgtcg ctgctgcaca ccatgaaagc gggcttcggc
     5581 aacacgctgg gagaactggc tatcatcgtg gtgttcggtg cggtcatcgg taaattgatg
     5641 gtcgactccg gcgcggctca ccagatagca catacgctgc tggcgcgtct cggtctgcgc
     5701 tatgtacagc tgtcggtgat tatcatcggc cttattttcg gtctggcgat gttctatgaa
     5761 gtggccttta tcatgttagc gccgctggtt attgttattg ccgccgaagc taaaattccg
     5821 ttcctgaaac tggcgatccc ggcagtagca gctgccacta ccgcacattc actgttccca
     5881 ccgcagccgg gtccggtggc gctggtgaat gcttatggcg cggatatggg gatggtttat
     5941 atctatggcg tactggtgac gatcccaagt gtaatctgcg caggtctgat cctgccgaag
     6001 ttcctcggca atcttgagcg cccaacgcca tcattcctga aagcagatca accggtagat
     6061 atgaataatc tgccctcttt cggcgtttcg attctggtgc cgctgatccc ggcgatcatt
     6121 atgatctcta ccaccatcgc caatatctgg ctggtaaaag atacccctgc ctgggaagtg
     6181 gttaacttta ttggttcctc gccgattgcg atgtttattg cgatggtggt tgcattcgta
     6241 ctctttggca ccgcgcgtgg tcatgacatg cagtgggtga tgaacgcttt tgaaagcgcg
     6301 gtgaagagta ttgcaatggt gattctgatc atcggtgcgg gtggcgtgct gaagcagacc
     6361 atcatcgaca ccggcattgg cgacaccatc ggcatgttga tgtcccacgg caatatctcg
     6421 ccctacatta tggcatggct gatcactgtg ctaattcgtc tggcgacggg tcagggtgtc
     6481 gtttcggcga tgaccgccgc cgggattatc agtgctgcaa tccttgatcc agcaaccggt
     6541 cagctggttg gcgtgaatcc ggcgctgctg gtactggcga cggctgcggg ttccaacacc
     6601 ctcacccaca ttaatgatgc atctttctgg ttgttcaaag gttactttga cctgtcagta
     6661 aaagacacgt tgaaaacctg gggtctgctg gagctggtca actccgtggt tgggctgatt
     6721 attgtgctga ttattagcat ggtagcgtaa aagaacacca gagcctgcca atggcaggct
     6781 cagactgatg actaaagtct aaaaatgtgc ccggacagtc ccctcgcccc tccggggaga
     6841 gggttagggt gaggggaaca gaccagtaaa gtgcgctttg tttatgccag atgcgacgct
     6901 gacgcgtctt atctggccta caaagggcta acgtgcaggt tttgtaggtc tgatagcgta
     6961 gcgcctcagg taccagcatt agcaatgcgc tgtaattttt ttattgataa acaaaagtca
     7021 cgccaataat cgattgcaca ttccctgcag tcacctgccc tccggtacgt gcgtaatttg
     7081 ccgttaatcc cagacttacc gccgaagttc ctactgctcc taacgatacc gtgttattcg
     7141 ctggaataat cgtaccgttg cgcgtcaact gtacgccgac gccctgcgct ggtgaaaacg
     7201 acgcggtatt ggtgaaaatc gagttgcccg catctgcggt tgtgccggag aggtaatacc
     7261 ccaggttttg gcttttcgca caataaacgg taagaggaat tggcactgaa ccagggtagt
     7321 ccggcagagt aacggtgaca tcacgagcag aaacatcaca accgccagtg ggcaccacca
     7381 catcattatt ggcgtaaata ttccacacaa actgaaaatc atcgctgtta tagttgttgg
     7441 tctgtcgcaa aataagcacg gcaattaatg aaccagcttt aatagccacc ccgcccgcac
     7501 tgctcacagg cgtcaaataa agcgccaccg gccacggctt atccgttctc gaattataaa
     7561 caacccgcgg cgtttcgctg gtggtcggaa atggatagct actgccacta tattttacgg
     7621 tcccggaaaa attagataac acgccgccgt aagccgagcc tcgttgcagt gtgacatagt
     7681 ctgtaatggt ttccggataa tcgttatggc aaaagatttg cgtcgaaaga tctacgacca
     7741 ggttttgccc cacattcacg acaggcgcaa ggtttacata aacattagcg ctgccaccgc
     7801 caatagggat agcggtacca ttggcggttt tacaggcgaa tgaccaggca tttaccgacc
     7861 agcccatcag cagtacagca aacagggtaa tagctcgttt cattacaatt atctcttcgg
     7921 gttcagctgt aggtataggt gatgctaatc actgcctgaa tggttccctg agtggctccg
     7981 ccatttaccg tcaatgctct gacctgtaac gggaagtgcg ctgattgtga ggaatcatcc
     8041 acctgaactg ttttggttgc gccagtattc aatgtgttgc cactgtcatc ctgtagctct
     8101 aactggatgt tttgcgcggt cccctggttt ttataatatc cggtgctgtc ggctgccccg
     8161 ctgaagctgg cagtgaccct cgacgttccc accggacaat tagtcaactc aagcgcaaca
     8221 tcatgccagg ccgatgccgc cccggcagac ataagactga aagaataaag atcgccgaga
     8281 tctaccgtgg cattggtggt ggaaaccgta cacggtttgg cgacgacctt accgttcacc
     8341 gtgatggtga catcggctgc ctgtatcgtc gcacttgcga gcgccaacat tgccgccaat
     8401 acatacccac gtttgcacca tttcatgagc atctccagtt actgatattc aagagtgaag
     8461 gtagccgtag cattgatatg ccccgcagtg acaggcacct gtgtcgccat tagccgggcg
     8521 taaaaattca gcgtatttgg tttacccggc gtcagggtcg tccacgaaat cgcagacgat
     8581 ggagcattaa ggggtatttg attttgctgc tcattcagaa gctgtattcc cagtcccgca
     8641 gccgctgaca ccgtattttc aagtgcaagc aggttggcat tgtggctatc tgcaacgccg
     8701 gtaaacccaa cttttacggc agaaacggca ttaccacagg gtgacagcaa aatacgaaat
     8761 ggaacgacag gagtcgtcgc gccaatgttg ttaaattgct tcgccgcgtt ttccatcaga
     8821 tcaacagtaa aattggttga ttcagcggcc acactacagc cgttatctct gacatagccg
     8881 cggatagtaa tcgtgctatc cgcagccaaa acgcgactta ccgccagcca caaaaaagcg
     8941 cacagaagat aaaaaggttt gtttctcatc acgccccctt aacgacattc agctgatagc
     9001 tgggttaata actgctgctg actctctggt ggcagttgat aattggcgac acagtgagca
     9061 ttttcctctt ctccccattt cacctgaact tttcccgcta aaggcattcc gctgaggtaa
     9121 acctgaccat tatccgcaac aatgccgcta ctctggctac tctctgatgt caccatcgcc
     9181 ccaaacggca gcggcttatt attgtgagtt agcgtcatga gcagttttat cccaacgcgc
     9241 gctttaaact ctgctcgcac gatcgcccca cgagtgggaa caacgttagc gaccgcgtta
     9301 tctaaatcga cgttatcagc caaggtattg gtatccagcg ccactctatt ttcccgatat
     9361 tcagtggcat aaggcagcac ggcataacca cgccagtcgg tacgcacccc cgtctggttt
     9421 tcgacttttg catcttttgc gccaggcgct ttaacaagca ccaccgtatc gtttaacggc
     9481 tgccccagcg ttacgccatt ggcatgagcc aataccccac cgctgactcc gtaatagagc
     9541 tgcttaatat catcgctatg gctgtaaccg atattggcat tgccgtaacc accgcgataa
     9601 ttcagcgtgg cgtagcctgt acttccgcta ttaccatcgc ctcccccggc atagccggtt
     9661 tgcacgctat aactgaggtt gttgtcttcc agcaacgtac cgtatacacc agccagattg
     9721 gtcatccgac cgttgagatc gtgtgacatg ctgtagctgg cactggcatg tcgccactga
     9781 gatttactgt cagaacgcag ccagtggctg aaaggaatat tgacgttaag cgctaacatc
     9841 tgatcccgtc ctttttgcca ggcgtttttc gtcaggctat agctgagcgt ccagttgata
     9901 tcttcgaacg cagtatttaa tccagcctgg aattgctcat cgacattact cgttccccaa
     9961 taagtttgat ggctaccact caaatacagt gttgatgtgc gcccgagttg ctgagtaacg
    10021 gtgagctgta atttcccgcg tttgttataa gcgaggttgt aatagtcggt gaatttcggc
    10081 ttaacctgaa taactccatc ctgtgtttcg atgttgtagc cattcattcg actgtatgtt
    10141 gtatcagcga aattaaaata tccgctggtc gaataacggt aacccactaa ctgaatattc
    10201 gtgcctgatt cattgagcga tttgttatag agaaaacgca ccgattgtcc gtcatgctga
    10261 ctgtcatcgg gaagtgtgga attagcctgc gtcatatcca cagacagagc gcccagtgcc
    10321 tccatgtttt tcccgatacc gaaattaaaa gcacgataac gatccgccag ttgcgttcca
    10381 ccatatattg tccagccagc cggaaggccg tggagtaatg tactctggaa aaagcggggt
    10441 ttttcctgct gcgcatttcc actacggtat tctcctgccg taatggaata acgagtatgc
    10501 ccttcacgtt gcaaaagcgg gactgacgaa tagggtacgg taaaaatctg cgtgctgccg
    10561 tcagcctctt tgatcgttac ctgcaagtca ccactattac ctgcggcata gatatcgttg
    10621 atggtaaaag gccccggtgg caccgtacta ttataaatgc catatccatt ttgtttaata
    10681 gtgacctgtg cagtaccacg agcaataccg tggatcaccg gggcaaatcc tctttggcta
    10741 tcgggtaaca tattgtcatc tgaggccaat tgtgcgccgc gaaagttaat accatcgaaa
    10801 atatcgccct gggtataacc atcacccaac gtcagccggg aacgtaaagg aattatgtct
    10861 cgctcaatcc aggtaatgat atgctgccat ttatttttgc tacctgatga actgtcgcta
    10921 ctgttataac tccaggtggt attgtcgcgt aaacgccacg caccaatatt taacccactc
    10981 tgtaggttta aatatgcata atggctgtta cccccaatcc g
//
LOCUS       DAJTQQ010000114        10889 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000114 DAJTQQ010000000
VERSION     DAJTQQ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.114"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..435)
                     /locus_tag="OO309_003420"
     CDS             complement(<1..435)
                     /locus_tag="OO309_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5848438.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFD"
     gene            complement(466..1158)
                     /gene="fimC"
                     /locus_tag="OO309_003421"
     CDS             complement(466..1158)
                     /gene="fimC"
                     /locus_tag="OO309_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5848439.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1378..1920)
                     /gene="fimA"
                     /locus_tag="OO309_003422"
     CDS             complement(1378..1920)
                     /gene="fimA"
                     /locus_tag="OO309_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5848440.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2401..3267
                     /gene="folD"
                     /locus_tag="OO309_003423"
     CDS             2401..3267
                     /gene="folD"
                     /locus_tag="OO309_003423"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR5848441.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3269..3481
                     /gene="ybcJ"
                     /locus_tag="OO309_003424"
     CDS             3269..3481
                     /gene="ybcJ"
                     /locus_tag="OO309_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR5848442.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3589..4098
                     /locus_tag="OO309_003425"
     CDS             3589..4098
                     /locus_tag="OO309_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5848443.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVLAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4134..5519)
                     /gene="cysS"
                     /locus_tag="OO309_003426"
     CDS             complement(4134..5519)
                     /gene="cysS"
                     /locus_tag="OO309_003426"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR5848444.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5693..6187
                     /gene="ppiB"
                     /locus_tag="OO309_003427"
     CDS             5693..6187
                     /gene="ppiB"
                     /locus_tag="OO309_003427"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR5848445.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6190..6912
                     /gene="lpxH"
                     /locus_tag="OO309_003428"
     CDS             6190..6912
                     /gene="lpxH"
                     /locus_tag="OO309_003428"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR5848446.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7030..7539
                     /gene="purE"
                     /locus_tag="OO309_003429"
     CDS             7030..7539
                     /gene="purE"
                     /locus_tag="OO309_003429"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR5848447.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7536..8603
                     /gene="purK"
                     /locus_tag="OO309_003430"
     CDS             7536..8603
                     /gene="purK"
                     /locus_tag="OO309_003430"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR5848448.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8740..9633)
                     /locus_tag="OO309_003431"
     CDS             complement(8740..9633)
                     /locus_tag="OO309_003431"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR5848449.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9630..10277)
                     /locus_tag="OO309_003432"
                     /pseudo
     CDS             complement(9630..10277)
                     /locus_tag="OO309_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10280..>10889
                     /locus_tag="OO309_003433"
     CDS             10280..>10889
                     /locus_tag="OO309_003433"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HCR5848450.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSI"
BASE COUNT         2615 a         2843 c         2782 g         2649 t
ORIGIN      
        1 atcaaagtta attgtggcat caggtacagc tttcagtaaa ttgatgcatt tattgttttg
       61 ctgctgggct aactcgggaa atgcactgct attaaggcca attcgttcaa ggagtacctg
      121 gttaaaacag ggcattaacc cacctgattt atcacctgta ttttctgttg tcgattcaaa
      181 tacgatatcc tgtgaaccaa tgaactcatc attacgccag agatccaccc gatacacgcc
      241 cgcaggttga tgattacctc tctcaaagcg cgataaatca gcaacggatg tgccattctc
      301 taataaaaat gccgggttga aataactttc cgcatagctt aaaaaggagg gtaaaatggc
      361 aagagatgaa taacaaattc cggccagaca ccaggtatac ctctgcggaa tatccgtagt
      421 agtgggtatt ttcataatta gctctgatat tgtcagatga ttcggttagt ttaagttcac
      481 ttctctgacc ggggttactg aaccataatc attaacggtc tgaaatttca gctttccctg
      541 cactcctgag ggtaagggaa tttgtgaact aactctgggt gccgccatag cattccccaa
      601 cttttggctg cccacttgca ggttaacgag ggtgacataa aaaggtgatg gattatgaac
      661 attgatatag ttaccggatc gcgaaaactt gagtgtgtcc ggcgcttctg cgggtaattc
      721 ttgtaattga attggacgga gaaataattt catgcgcgat aaaatcgcca gttgcaaaac
      781 attcctgccg ttcaatgcat ttttatctac cgaagggatc gtcttaacat tcatccagaa
      841 cagagactca cgatctgccg ccagcggtgg accggtgtaa ataatacgca aagtattttc
      901 gcttttgggt tcactaacaa atagtggcgg tgtaatgatg aatgactttt cttttacacc
      961 gctgctgttt tcaatccacg aattgaccag aaagagctca ttggtatggc tatttttaat
     1021 ccataccgca gtctgtttag catcagcggg ataaataata cgcgtggcac ctaatgcgat
     1081 ccctccggca gcatgggcgc tggccgaaat gattaaataa aatatcatga gcatcaataa
     1141 ctttatttta gtcatcatag ttaatacgcc ttgtgtattc ctgacggcaa aaataaggag
     1201 tataaagaaa tgattgcttt ggcttcttac aacacatcat tcccttttca tttaataaag
     1261 cggcatgata caaagtgaac gtggtttcga tttacattgc caccataccg cttcagacaa
     1321 ccggtacatt actttgttat aagacgtgat atataattca aaacaacgtg gttttgatta
     1381 ttcgtatctc ataataaaag tcgcgtcagc atttgcttgc cctgctgatg cacttgtacc
     1441 ggtgccttta taacgtgcag aaaaatgaag aacgttggtc ccggggatca agttctggtt
     1501 ggttgagaag ctattcccat caggtttgag aattgcggat gtattatcaa gtatttcaat
     1561 accgacaccc gttgctgttg ttgtatttgt actggataca atggccaata aattatcatt
     1621 gattgcatca gcctgaccag aaaatgcaac ggcagcatta gcggcaacaa caggatcgca
     1681 gtcgttcaac tgaatggtga atggaattaa tgcagaggta ttaccaacag cattgaaaat
     1741 atcggtacga tattgaccga gtgtgacaac ctggtctgcc gagtcagtat tcactgaaca
     1801 ggcagcattc accagttcgc cttcaaagtg aattgtaccg ccatctacaa cggcagcata
     1861 actacccgta gcggcgaata gtgcggcagc cagcgcagac gaaataaatc ttaatttcat
     1921 atatattcct tcaatctcat ttatcgactc cacatccgta tataaccgat tactttattt
     1981 aagacactga tagtagtaaa attccttttt atcctctaag aatgtcttaa ttgaaaatat
     2041 gcactctatt ctaaaaaata gagagccccg ttagatgaat acttccgcgc aaaatatatt
     2101 caccacaaat atagacctga agcggtaaat taccaggctg aaaattcttt ttatattgtc
     2161 aggtatttct taaattatct taatccttaa acaaggaaat aaatcagttc cagatttaca
     2221 acgccatcat gtacgaaaaa tgaagctttc aatctcagcg acctcagcga cggtgcgcct
     2281 aaccttcgca agaggtcgct tcacgcgata aatctgaaac gaaacctgac agcgcgcccc
     2341 gcttctgaca aaatagacgc atccccttcg acctacgtaa cagatggaat cctctctctg
     2401 atggcagcaa agattattga cggtaaaacg attgcgcagc aggtgcgctc tgaagttgct
     2461 caaaaagttc aggctcgtat tgcagccgga ctgcgggcac caggactggc cgttgtgctg
     2521 gtgggtagta accctgcatc gcaaatttat gtcgcaagca aacgcaaggc ttgtgaagaa
     2581 gtcgggttcg tctcccgctc ttatgacctc ccggaaacca ccagcgaagc ggagctgctg
     2641 gagcttatcg atgcgctgaa tgccgacaac accatcgatg gcattctggt tcaactgccg
     2701 ttaccggcgg gtattgataa cgtcaaagtg ctggaacgta ttcatccgga caaagacgtg
     2761 gacggtttcc atccttacaa cgtcggtcgt ctgtgccagc gcgcgccgcg tctgcgtccc
     2821 tgcaccccgc gcggtatcgt cacgctgctt gagcgttaca acattgatac cttcggcctc
     2881 aacgccgtgg tgattggcgc atcgaatatc gtcggtcgcc caatgagcat ggaactgttg
     2941 ctcgcaggtt gcaccaccac cgtgactcac cgcttcacta aaaatctgcg tcatcacgta
     3001 gaaaatgccg atctgttgat cgttgccgtt ggcaagccag gctttattcc cggtgactgg
     3061 atcaaagaag gcgcaattgt gattgatgtc ggcatcaacc gtctggaaaa tggcaaagtt
     3121 gtgggcgacg tcgtgtttga agacgcggct aaacgcgcct catacattac gcctgttccc
     3181 ggcggcgttg gcccgatgac ggttgccacg ctgattgaaa acacgctaca ggcgtgcgtt
     3241 gaatatcatg atccacaggg tgagtaacat ggcgacattt tctttaggta aacatccgca
     3301 cgttgagctg tgcgacttgc tgaaactgga aggctggagc gaaagcggcg cgcaggcgaa
     3361 aatcgcgatt gccgaaggcc aggtgaaagt cgacggtgcg gttgaaacgc gcaaacgctg
     3421 caaaatcgtc gccggtcaga cagtgagttt tgcaggacac agcgtacagg ttgttgcctg
     3481 atccctttcc cggcctgtat caacaggccg gttctctttt cagacttttg ccctctacgc
     3541 cccgttcatc catgctgata gcatcgacat tttgccgaag cgaccatcat gccaaccgtt
     3601 attacgcacg ccgccgttcc cctttgcatt ggtttagggt tgggttcgaa aggtctgtta
     3661 tttgccggaa tcatcctggc gatgctgccc gatgccgacg tattgtcgtt taaatttggc
     3721 gttgcttacg gcaatgtttt tggtcatcgc ggttttaccc attcgctggt gcttgcgttt
     3781 gttgtcccgc tcttatgtgt gctgattgga cgtcgatggt tcagggctgg gctgattcgc
     3841 tgctggctgt ttttaaccgt ctcgttgcta tcgcacagct tactggattc ggtaaccact
     3901 ggcggtaagg gcgttggctg gctgtggccg tggtcagatg aacgcttttt cgctccctgg
     3961 caggtgatta aagtcgcgcc gtttgcattg tctcgttaca ccacagcgta cgggcatcag
     4021 gtgattattt ccgaattgat gtgggtgtgg ttgccgggga tgctactgat gggaatgttg
     4081 tggtggcgca gacgataacc ggatgcgaaa actcgcatcc ggcaattgcg caattactta
     4141 cgacgccagg tggtcccttg cgggccatct tccagcacga tccccatctc gttaagacgg
     4201 tcacgtgccg catctgccgc cgcccagtct ttcgctttac gggcatccag acgctgttga
     4261 attaacgctt caatctcagc cacttcgctg tcgtctgcct gcgcgccgct ttgcaggaac
     4321 gcttccggtt cttgctccag caggcccaat acagcggaaa gtttacgcag gtgagatgcc
     4381 attgcattcg ccgctgccat atcttctgct ttcagacggt tcacttcacg cgccatatca
     4441 aacagcacgg aataggcttc cggggtgttg aaatcgtcgt ccatcgcttc aataaagcgc
     4501 gcttcaaacg cttcgccacc ggcaggcgca acggttttat ctgtgccgcg cagcgcagtg
     4561 tagaggcgct ccagcgccgc acgcgcctgc ttcaggttct cttcgctgta gttcagctgg
     4621 ctgcgatagt ggcccgacat caggaagtaa cgcacggttt ccgcgtcgta gtatttcagc
     4681 acatcgcgca cggtaaagaa gttacccagc gatttggaca tcttctcgcg gtcaaccatc
     4741 accattccgg agtgcatcca gtagttcaca tactgaccat catgggcaca ggtggactgc
     4801 gcgatttcgt tctcgtggtg cgggaacatc aggtctgaac cgccgccgtg gatatcaaag
     4861 tggttaccca gctgcttgca gttcatggcc gaacattcaa tgtgccagcc aggacgaccc
     4921 gcgccccacg gagacggcca gctcggttcg ccctctttcg acatcttcca cagaacgaag
     4981 tccatccggt tgcgtttgtc atcaaccacg tcaacgcgcg cgccagcctg cagctggtcg
     5041 agatcctgac gcgacagcac gccataagtt ggatcggtcg ggacgtcgaa catcacgtcg
     5101 ctgttgtccg ccacataagc gtgacctttg gcgatcagtt gttcagtgag ttcaataatt
     5161 tctgcgatat ggtgcgtcgc gcgcggctcc atatccgggc gcagaatgtt caaagcatca
     5221 aaatctttgt gcatttcggc gatcatgcga tccaccagcg ccacaaagct ttcgccattt
     5281 tcattggcgc gtttgatgat tttgtcgtcg atatcggtaa tgttgcgcac atacttcagt
     5341 ttatagccga ggaaacgcag atagcgcgca accacgtcaa aagcaacaaa ggtacgcccg
     5401 tgaccgatat gacagagatc gtaaacggtg attccacaca cgtacatgcc gacttccccg
     5461 gcgtgaatag gcttaaattc ctctttttgg cgtgtcagag tattgaagat ttttagcatc
     5521 gaagattccg tttagacatg tgtgggtaat tgagttgcgt ataataccca tatttcccgc
     5581 gcgaatcagc atacattgcg tgatgatccg atcgtgcggt tatgctataa caccacccta
     5641 tatatgaccc gaactgggtt gaagcaccaa tcaaacggaa caggatgcaa aaatggttac
     5701 tttccacacc aatcacggcg atattgtcat caaaactttt gacgataaag cacctgaaac
     5761 agttaaaaac ttcctggact actgccgcga aggtttttac aacaacacca ttttccaccg
     5821 tgttatcaac ggctttatga ttcagggcgg cggttttgaa ccgggcatga aacaaaaagc
     5881 caccaaagaa ccgatcaaaa acgaagccaa caacggcctg aaaaataccc gtggtacgct
     5941 ggcaatggca cgtactcagg ctccgcactc tgcaactgca cagttcttca tcaacgtggt
     6001 tgataacgac ttcctgaact tctctggcga aagcctgcaa ggttggggct actgcgtgtt
     6061 tgctgaagtg gttgacggca tggacgtggt agacaaaatc aaaggtgttg caaccggtcg
     6121 taacggtatg caccaggacg tgccaaaaga agacgttatc attgaaagcg tgaccgttag
     6181 cgagtaatcg tggcgacact ctttattgca gatcttcatc tctgcgtgga agaaccggcg
     6241 atcaccgccg gttttctgcg ttttttagcg ggggaagccc gcaaggccga cgcgctgtat
     6301 attcttggcg atctgtttga agcatggatt ggcgacgacg atcccaaccc actccatcgc
     6361 cagatggcgg cagcgatcaa agcggtgtcc gattccggcg ttccctgtta tttcattcat
     6421 ggcaaccgtg attttctgct cggcaaacgc tttgcccgtg aaagcggcat gacattattg
     6481 ccggaagaaa aggtgctcga actttatggt cgccgggtgt tgattatgca tggcgacacg
     6541 ctgtgcaccg atgacgcggg ttatcaggct tttcgcgcca aagtccacaa accctggctg
     6601 caaatgctat tcctcgccct gccgttgttt gtgcgcaaac gcattgccgc gcgaatgcgc
     6661 gcgaacagca aagaagccaa cagcagtaaa tcgctggcga tcatggacgt taaccaaaac
     6721 gctgtggtca gtgcgatgga aaaacatcag gtgcaatggc taatccacgg gcatacccat
     6781 cgcccggcgg tgcatgaact tatcgccaac cagcaacctg cttttcgcgt ggtactgggt
     6841 gcctggcata cggaaggttc gatggtgaaa gtcacggctg atgacgttga gctgattcat
     6901 tttccgtttt aaaaaaccca caattttgct gatttcacag ccacgcaacc gttttccttg
     6961 ctctctttcc gtgctattct ctgtgccctc taaagccgag agttgtgcac cacaggagtt
     7021 ttaagacgca tgtcttcccg caataatccg gcgcgtgtcg ccatcgtgat ggggtccaaa
     7081 agcgactggg ctaccatgca gttcgccgtc gaaatcttcg aaatcctgaa tgtcccgcac
     7141 cacgttgaag tggtttctgc tcaccgcacc cccgataaac tgttcagctt cgccgaaagc
     7201 gccgaagaga acggttatca ggtgattatt gcgggcgcag gcggcgcagc gcacctgcca
     7261 ggcatgattg ccgccaaaac gctggtgccg gtgctgggcg tgccagtaca gagcgccgca
     7321 ctgagcggtg tcgatagcct ctactccatc gtacaaatgc cgcgcggcat tccggtgggt
     7381 acgctggcga ttggtaaagc tggcgcggca aacgcggcat tactggcagc acaaattctc
     7441 gcgactcacg ataaagagct acaccagcgt ctgaatggct ggcgcaaagc ccagaccgac
     7501 gaagtgctgg aaaacccgga cccgcgaggt gcggcatgaa acaggtttgc gtcctcggta
     7561 acgggcagtt aggccgtatg ctgcgtcagg caggcgaacc gttaggcatt gctgtctggc
     7621 cggtcgggct ggacgctgaa ccggcggcgg tgccttttca acaaagcgtg attaccgctg
     7681 agatcgaacg ctggccggaa accgcattaa cccgcgagct ggcgcgtcat ccggcctttg
     7741 tgaaccgcga tgtgttcccg attattgctg accgtctgac tcagaaacag cttttcgata
     7801 agctccacct gccgaccgcg ccgtggcagt tacttgccga tcgcagcgag tggcctgcgg
     7861 tgtttgatcg tttaggtgaa ctggcgattg ttaagcgtcg cactggtggc tatgacggtc
     7921 gcggtcaatg gagtttacgc gccaatgaaa ccgaacagtt accggcagag tgttacggtg
     7981 aatgtattgt cgagcagggc attaacttct ctggtgaagt atcgctggtt ggcgcgcgcg
     8041 gctttgatgg cagcaccgtg ttttatccgc tgacgcataa cctgcatcag gacggtattt
     8101 tgcgcaccag cgtcgctttt ccgcaggcca acgcacagca gcaggcgcaa gccgaagaga
     8161 tgctgtcggc gattatgcag gagctgggct atgtgggcgt gatggcgatg gagtgttttg
     8221 tcaccccgca aggtctgctg atcaacgaac tggctccgcg tgtgcataac agcggtcact
     8281 ggacacaaaa cggtgccagc atcagccagt ttgagctgca tctgcgggcg attaccgatc
     8341 tgccgttacc gcaaccagta gtgaataatc cgtcggtgat gatcaatctg attggtagcg
     8401 atgtgaatta tgactggctg aaattgccgc tggtgcatct gcactggtac gacaaagaag
     8461 tccgtccggg gcgtaaagtg gggcatctga atttgaccga cagcgacaca tcgcgtctga
     8521 ctgcgacgct ggaagcctta atcccgctgc tgccgccaga gtatgccagc ggcgtgattt
     8581 gggcgcagag taagttcagt taagcaggtg ctcatttgat tgccggatgc ggcgtaaacg
     8641 ccttatccgg cttaccgctc cggtacccat tgtaggcctg ataagacgcg tcaagcgtcg
     8701 catcaggcat ggtgcaccga ttgccggatg ctctcatcac tacagcgaaa tacaggtccc
     8761 cgcttcgccc gccagcgtct cttcaattcg cgataacgcc ccaatccacg cgggtttact
     8821 acggcttctg acataaccac tcaccgccgt taccttcggc cccatcgaac catcggcttt
     8881 ggcaaatggc gctaactcat ccggtgtggc atggcgaatg gcacgttgct gcggcgttcc
     8941 ccagttttca tataccgcat cagcatcggt gaggatcacc agtccatcgg cattaatctg
     9001 ctcggcgagc aacgcagcgg cgagatcttt atcaatcacc gcttcactcc ctgccccatc
     9061 ttccgtcaca ggcacaccgc cgccgccact gcaaatcacc acatgcccct ctttgagcaa
     9121 caactcgatg gcttcgctgt cgagaatttt acgcggttgc ggagacgcca ccacccggcg
     9181 caaatattta ccatcacgtt tcatctgcca gccgtaagcc gcttccagtg cctcttgttc
     9241 ttctggctga taaaccggac caataaattt ctctggctgc aaaaacgccg gatcatcagg
     9301 cgaaacctca atgcgcgtca gcaccgtcgt aacaggcggc atctgcggct gtgcgctcaa
     9361 gctctgcgcc agcatatagc caatcatccc ctggctttcc gccaccagta catccagcgg
     9421 atacggttct acctctttcc acgccagatt ctgtaacgcc agcagcccca cctgcggccc
     9481 gttgccgtga acaatcgcca gccgataaga acgggccagg cgtgccagcg cgggtacagc
     9541 actggcgata ttgcgatatt gattttccgc cgtcagcgcc tcaccgcgct ggagtaaggc
     9601 gttgccccca agagctacaa ccagtgtttt catggttttc cttgtaataa ttgttggcca
     9661 agccagaacc ccagcagcgt atctgcccct gacgtatgcc ccagcgccag cagcgaatca
     9721 atcgcaacag cggtacgttt cgggcaactc agagcatgga caaagtgcag gagtggcgag
     9781 gcgaaatatc cttgcgcggc ataacgtaaa taactgacgc tcaccgctgt ggtaacgagt
     9841 tgaagattgt cggaacaggc aaaaaacggg cgaccggcgc gcgcatctaa agcgccataa
     9901 taccaggccg ccagcagcat tccgctcaac gtgtcatcat gacttggcgt taatccgggg
     9961 cctttaccca gccagtgccg ccagtcggtc ttaacgccat tgagcgcggc ctgaaaacag
    10021 tgacgaaact ggcgtaattc agcgggcagc ggatcgcttg ccgccatcgc cagtggcccg
    10081 aaaagtccgg tttcctccgc gcgttgcatc catgcagctg caagcggttg aggatgcgaa
    10141 ggcggcgtaa tacgcagcaa acaataacgc tgtggctgtt taaccgtgaa acgcccgagg
    10201 cgaatccctt gagccacaac ctgtggtcgt tcattgccgc ataatccacc gcataacgca
    10261 tcgaattgcg tgcggcgaaa ttacacattc ccgtcaagct atagaagaaa cgctgccctg
    10321 gctttcctct tttggcgctg acccaacggg tgggatgacc cgtttacttt attcgccgga
    10381 atggctggaa acccagcagc aatttaaaaa aagaatggca gcaagtgggc tggaaacacg
    10441 tttcgatgaa gtggggaatt tatacggtcg cctgagtggc accgaatatc cacaggaagt
    10501 ggttctgagc ggttcgcata tcgataccgt ggttaacggc ggtaacctcg acgggcaatt
    10561 cggcgcgctg gcggcgtggc tggcaattga ctggttgaaa acgcaatacg gcgcaccgtt
    10621 acgtacggtc gaagtggtgg cgatggcaga agaagaaggc agccgcttcc cgtatgtctt
    10681 ctggggcagt aaaaatatct ttgggctggc gaatcctgac gacgtgcgga atatctgtga
    10741 tgccaaagga aatagttttg ccgatgcgat gaaggcttgc ggatttactc tgccgaacgc
    10801 cccactaact ccgcgtcagg ctataaaagc ctttgttgaa ctgcatattg aacagggctg
    10861 tgtgctggaa agtaatgggc aatcaattg
//
LOCUS       DAJTQQ010000115        10887 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000115 DAJTQQ010000000
VERSION     DAJTQQ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10887
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.115"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..203
                     /locus_tag="OO309_003434"
     CDS             <1..203
                     /locus_tag="OO309_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848451.1"
                     /translation="GASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAI
                     YLLAGCLISAMTALLMKDNQRS"
     gene            305..1759
                     /gene="amn"
                     /locus_tag="OO309_003435"
     CDS             305..1759
                     /gene="amn"
                     /locus_tag="OO309_003435"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR5848452.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2102..2818
                     /locus_tag="OO309_003436"
     CDS             2102..2818
                     /locus_tag="OO309_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5848453.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3274..3349)
                     /locus_tag="OO309_003437"
     tRNA            complement(3274..3349)
                     /locus_tag="OO309_003437"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3314..3316),aa:Asn,seq:gtt)
     gene            complement(3450..5077)
                     /gene="yeeO"
                     /locus_tag="OO309_003438"
                     /pseudo
     CDS             complement(3450..5077)
                     /gene="yeeO"
                     /locus_tag="OO309_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            5082..5157
                     /locus_tag="OO309_003439"
     tRNA            5082..5157
                     /locus_tag="OO309_003439"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5115..5117,aa:Asn,seq:gtt)
     gene            complement(5195..6145)
                     /gene="cbl"
                     /locus_tag="OO309_003440"
     CDS             complement(5195..6145)
                     /gene="cbl"
                     /locus_tag="OO309_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR5848454.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6247..7164)
                     /gene="nac"
                     /locus_tag="OO309_003441"
     CDS             complement(6247..7164)
                     /gene="nac"
                     /locus_tag="OO309_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR5848455.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7492..7567
                     /locus_tag="OO309_003442"
     tRNA            7492..7567
                     /locus_tag="OO309_003442"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7525..7527,aa:Asn,seq:gtt)
     gene            complement(7623..8558)
                     /gene="ldtA"
                     /locus_tag="OO309_003443"
     CDS             complement(7623..8558)
                     /gene="ldtA"
                     /locus_tag="OO309_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5848456.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8620..9699)
                     /gene="cobT"
                     /locus_tag="OO309_003444"
     CDS             complement(8620..9699)
                     /gene="cobT"
                     /locus_tag="OO309_003444"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR5848457.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9711..10454)
                     /gene="cobS"
                     /locus_tag="OO309_003445"
     CDS             complement(9711..10454)
                     /gene="cobS"
                     /locus_tag="OO309_003445"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR5848458.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10451..>10887)
                     /gene="cobU"
                     /locus_tag="OO309_003446"
     CDS             complement(10451..>10887)
                     /gene="cobU"
                     /locus_tag="OO309_003446"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR5848459.1"
                     /translation="DEMAARIEHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLE
                     CVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGM
                     GIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         3023 a         2772 c         2354 g         2738 t
ORIGIN      
        1 ttggtgccag ttatcgctac agtggtgctg gagtcggtta tcaggttgcc agtgtggttg
       61 gcggtggatt tacacctttt attgccgctg cactcatcac ttactttgcc gggaactggc
      121 atagcatcgc catttatttg ctggctggat gcctgatttc tgcaatgacc gctttattga
      181 tgaaagacaa tcaacgctcg tgatagcctg gcgaagatca tccgatcttc gccttatact
      241 tttgtttcac attcctgtga catactatcg gatgtgcggt aattgtatgg aacaggagac
      301 acacatgaat aataagggct ccggtctgac cccagctcag gcactggata aactcgacgc
      361 gctgtatgag caatctgtag ttgctttacg caacgccatt ggcaaatata ttacaattgg
      421 cgaattacct gatgaaaacg cccgtaaaca aggtcttttt gtctatccat cactgaccgt
      481 aacctgggac ggtagcacaa caaatccccc caaaacgcgc gcatttggtc gttttactca
      541 cgcaggcagc tacaccacca cgattactcg ccctactctc tttcgttcgt atctgaatga
      601 gcaactaacg ttgctttatc aggattatgg tgcgcatatc tctgtgcaac cctcgcaaca
      661 tgaaatccct tatccttatg tcatcgatgg ctctgaattg acacttgatc gctcaatgag
      721 cgctgattta actcgctact tcccgacaac agaactggcg caaatcggcg atgaaactgc
      781 agacggcatt tatcatccaa cggaattctc cccgctatcg cattttgatg cgcgccgcgt
      841 cgatttttcc ctcgcacggt tgcgccatta taccggtacg ccagtagaac attttcaacc
      901 gttcgtcttg tttaccaact acacacgtta tgtggatgaa ttcgttcgtt ggggatgcag
      961 ccagatcctc gatcctgata gtccctacat tgccctttct tgtgctggcg ggaactggat
     1021 cactgccgaa accgaagcgc cagaagaagc catttccgac cttgcatgga aaaaacacca
     1081 gatgccagca tggcatttaa ttaccgctga tggtcagggt attactctgg tgaatattgg
     1141 cgtgggaccg tccaatgcta aaaccatctg cgatcatctg gcagtgctac gcccggatat
     1201 ctggttgatg attggtcact gtggcggatt acgtgaaagc caggccattg gagattatgt
     1261 ccttgcacac gcttatttac gcgatgacca cgttcttgat gcggttctgc cgcccgatat
     1321 acctattccg agcattgctg aagtgcaacg tgcgctttat gacgctacca agttagtcag
     1381 cggcaggcct ggtgaggaag tcaaacagcg gctgcgtaca ggtactgtgg taaccacaga
     1441 tgacaggaac tgggagttac gttactcagc ttctgcactt cgttttaact taagccgggc
     1501 cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt tccgcgtgcc
     1561 atacgggaca ctactgtgtg tttcagataa accgttacat ggtgagatta agcttcccgg
     1621 ccaggctaac cgtttttatg aaggcgctat ttccgaacac ctgcaaattg gcattcgggc
     1681 gatcgatttg ctgcgcgcag aaggcgaccg actgcattca cgtaaattac gaacctttaa
     1741 tgagccgccg ttccgataat aagttatgaa ccttcttatc agaaatagat gtgaaggatt
     1801 aagtaagaca tctggcaaat agcctgcaat aaacgcgggc tgcttgcttc cttgggcaga
     1861 tgcgagtttt attatcgtct taatgatttc cacatattaa aagcaagtat gctttcaaaa
     1921 cacaattata aaaaatcccg ccaacaacat tagtttttat aaaattaaat ataagattat
     1981 ggctttagaa tatttttatt tctaatagac gagatttttc ctgttatgat ataatatgcc
     2041 gaattaacac atgttaacga tttaccagta atgtaaataa attttcgagg agatcattcc
     2101 agtgggacgt aaatgggcca atattgttgc taaaaaaacg gctaaagacg gtgcaacgtc
     2161 taaaatttat gcaaaattcg gtgtagaaat ctatgctgct gccaaacaag gtgaacccga
     2221 tccagaatta aacacatctt taaaattcgt tattgaacgt gcaaagcagg cacaagttcc
     2281 aaagcacgtt attgataaag caattgataa agccaaaggc ggcggagatg aaacgttcgt
     2341 acagggacgt tatgaaggct ttggtcctaa tggctcaatg attattgctg agacattgac
     2401 ttcaaatgtt aaccgtacga ttgctaacgt tcgcacaatt ttcaataaaa aaggcggcaa
     2461 tatcggagcg gcaggttctg tcagctatat gtttgacaat acgggtgtga ttgtatttaa
     2521 aggaacagac cctgaccata tttttgaaat tttgcttgaa gctgaagttg atgttcgtga
     2581 tgtaactgaa gaagaaggta atattgttat ttatactgaa cctgctgacc ttcataaagg
     2641 aatcgcggcg ctaaaagcag ctggaatcac tgagttctca acaacagaat tagaaatgat
     2701 tgctcaatct gaagttgagc tttccccaga agatttagaa atctttgaag ggcttgttga
     2761 tgcccttgaa gatgacgacg atgtacaaaa agtttatcat aacgtcgcaa atctctaatt
     2821 atattttaaa gaaatctgtc tttatggcag atttctttaa tctcatataa ttcttataaa
     2881 aaatataata tttaactcgt catattgctt ataaaaaatc cccgtcccca gagaaataat
     2941 atttattaaa atggcaggtc ttctttttct gattaccgaa gacaaagtgg caattacgta
     3001 tagtttcccg ataaagacgc gacagctacg tcccgcataa ggcatttttc tctttatctt
     3061 tgtacggtac ttcatggaac agaatttttg accttgtgaa tcgtgatatc tgttggggag
     3121 gggcaatttg ctcactgaag cgtgagactc gattaagcgc acgaaacaca gaaatcaaaa
     3181 aacccggcca cttttttaca aagtggccgg gtaaaaataa tttttatttt ttaactgtcc
     3241 tgagtctcat agagatgtct caaaactaaa atttggctcc tctgactgga ctcgaaccag
     3301 tgacatacgg attaacagtc cgccgttcta ccgactgaac tacagaggaa tcgtgagaac
     3361 gaggcgaata ttagcgatgc ccacccacaa tgtcaaagcc tgttttttaa atttgaaatc
     3421 gtttgctgaa ataatctgca ttttgtcgtt tattccgaca caactggctt tttttcacac
     3481 ttttgcggct cggatcgagg gtatttccac agccaacgtc cagtaaccat tcgccagtaa
     3541 aacagcacgg cccgcacagc ccagtcggca aacattccca tccagacacc aaccacaccc
     3601 cagccaagca tgattccctg cacataaccg actacaaccc ggcaacccca catgctcaac
     3661 atagaaaccc acatggcgta acgggcatca cgagcaccct taaatccagc gggtagcacc
     3721 catgaggcgg accaaatagg cataaataaa gcatttagct aaatcagaat cacaacgacg
     3781 tgtttaacct gtggatcctg ggtgtaaaac gatgccataa ccccggcaaa gggagccgtt
     3841 agccaggcga tggccgttaa tccaagagtg gacagccaga acacatgccg caactgaatc
     3901 tctgcttgcg ctatctgccc tacccccaac cttcggcctg taatgatcgt agaagcagaa
     3961 ccgagcgcac ttccgggtaa gttgataaga gccgcaattg agaacgcgat aaaatttccg
     4021 gcaataacgc tggtccccat cccggcaacg aacatttggg ttaataaccg accactggta
     4081 aataacactg attcgacact cgctggaata ccaatcccca tgacttccca gataatgcta
     4141 aaattcagcg gtttaaaata gctctttaac gaaatcctta gcgcaggatt aaaaccaatc
     4201 gccagcaccc acaaaattgc aactgcgcca atataacgag aaatggttaa acccaaccca
     4261 gccccgacca ggagaaaagg ccgtaaatca atatgccgct aataataata ttaagaatat
     4321 tcaggctacc gttaatcagt agcggtattt tcgtattccc tgcaccacga agtgccccgc
     4381 taccaataag agtgatggca gcggctggat aactgagtac ggtcagctcc agataagtca
     4441 acgccagtgc tttaacttct gtcgtggcat cacccgcgac gaaatcaata atttgttcgc
     4501 caaaatgatg aattagcgtt gccaacagta cggcaaacaa cgtcatgatc accaatgact
     4561 gccgcgtcgc caccctcgct cgtcgtcgat cccgcttacc gagactaaat gctacaacga
     4621 cagtagtacc aagatcgata gctgcaaaaa aagccataat gaccatattg aagctgtccg
     4681 ccaaccccac gccggccatc gcatcttttc ccagccaact gaccagaaaa gtgctcagga
     4741 cccccatcaa caggacacag gcgttctcca tgaagatagg aacagcaagc ggggttatct
     4801 cgcgctagaa caacactttg tagctcttgc gtttagagtg ccagcgagtg ccgtgaacaa
     4861 cctggcgtaa agcagaggag atattcaaag ccgaccttaa ttgcagaaag tgaaaccaca
     4921 tttcaaatga tgagggagaa tcagcaaagc tgcaaagatt ttcgccaaca aattgtctgc
     4981 aaatgcaaca aactgttgat agaaacggca aacagttggg aaatttaaaa atcgggtttg
     5041 acaaaatatt tttcgccgtt aagatgtgcc tcaacagcga ttcctctgta gttcagtcgg
     5101 tagaacggcg gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaaat
     5161 ttctgaaaag cccgctttta tagcgggctt tttgctatat ctgataatca atttcctctt
     5221 cactgttttc catcacctgg cgcttgatat cctcaactga cagtcctgca ttacaaagtt
     5281 ccagaaagcg ccaaacatag ttacgctgaa gttgtcctcg cttcagtccc aaccagacag
     5341 tattagcatc gaaaagatgc cgcgtatcca ggcggattaa attctcttcc tcttgttcgc
     5401 cgctggattg ttcggcaact aatccgatcc caagcccaag agcaacatag gttttaatga
     5461 catcagaatc ctgtgcactt aatacaatat ctgccggcaa acctttgcgg gtaaatgcgt
     5521 catcagtgcg tgagcgcccc gtaattccct gtcggtaagt aattaacggc cacttcgcta
     5581 ttgattccag cgtcaatggt gtaatttgcg ttaagggatg atcgagtgga acaagcaaac
     5641 tatggtgcca acaaaaccat gggaaggcga cgagctgcgg gtcattactc aaacgctcgc
     5701 tggcgatacc aatatcagct tcgccatttt gcaacaacgt cgcaatttcc tgtggcgaac
     5761 cctggattag ctcgagccga acctccggga aaagttcgcg aaaagcttta atgacctctg
     5821 gcaagctata acgtgcctga gtatgcgtcg ttgcaatagt gagaacacca gacgtatcgt
     5881 tggtaaacag gtctgcaagc cgacgaacat tactggcttc attcagaata cgttctgcaa
     5941 tgaccagtaa tgctttgccc ggttcagtca tccccagcag tcgcttacct cgtcgaacaa
     6001 atatttcgat gccaaattca tcctccagtt cccgaatatg acggctaacg cctgactgtg
     6061 aggtaaaaag catattcgca acctctgtca ggttgtaatc ctgacgtgca gcctcgcgga
     6121 ttatctttag ttgttggaaa ttcacggtaa actccgggca gttcagatat cccgttattg
     6181 ttaaagtcta atgcccggca taacaaataa taaaaacccg catcttattc catcccgata
     6241 taacgcttag ctcaccaatt gccactgcct tttttccatc actggagaac taatcactga
     6301 cattaacaac tctttcactg cctgtgcctg tggtgacaag tttgctctgg cgggtaaatt
     6361 taaggacaaa gagagactca ttgaaggagt ggtaatgcgt gacatccacc catttactgc
     6421 gccacataac gaacgcgcag ccgattcagg taatactgca acgcccatgc cgctggcaat
     6481 cgctgcggta agcgtggcaa tagactcaat ttcaccaatg acttttgccg tgagccgccg
     6541 tagggaaaaa gcctcatcaa cacgaagtct aacagcgctg taatcactgg ggagaaaaag
     6601 gttcatttgc gcaatagcat taacatcaac gttttgcccc gggcaatctt gagttcctac
     6661 caggaaaaga tcttctttca gcaaagcctg actggatacc ccagccacag gggaatgctc
     6721 ataaatcacc gccatatcga gttggtgatt tatcagtttt tcgttaagca ctgcaccact
     6781 attttcatga agatagataa cgacctccgg gaattcagcg cgaaccgcct gtaataaggg
     6841 catggtgatg gatgacgcag cggttcctgg cgcaaagcca atcgagactt gccccgataa
     6901 tgactgacca acgttatgca ccgccagttg ggcctgctca cactgacgta aaatggcccg
     6961 tgcatgggta tagagaattt ttccggcgtc tgttggcgta acgccccgtt ttgtgcgaat
     7021 caaaagctgt tgatttaact caccttccag tatggcaacc tgttggctga gcgctggttg
     7081 tgcgatatgc aatacttcag cagcctgggt caggctacca atatctacaa tttttacgaa
     7141 gtatttcagg cgtctgaagt tcatgttgcc tccggttttt aagaatcggc ccaagtgccg
     7201 ccattactta caaccagtat gcaagatgcc tgccagttct attttggcgt tgatgcgcaa
     7261 gctaaccaac tgtcaaataa gagattatga tagattcgtc attggccctt tttatcagca
     7321 gtcgcattca cctgccctat aaaaggaggg tatgcaccac gatggttcat tacccaataa
     7381 gattgaaagc tcaccacttt gttgaaattg gcagcaaaca aacaaaaaaa atgcatttca
     7441 ccctttgaca tcaccatgca ctgccattaa tatgcgcccc gttcacacga ttcctctgta
     7501 gttcagtcgg tagaacggcg gactgttaat ccgtatgtca ctggttcgag tccagtcaga
     7561 ggagccaaat tcaaaaaagc ctgctttcga gcaggctttt tactttctaa ttaccaacgc
     7621 tcttaaaaca tctgtcttga accagaacta atttgcacag gcattcccga tcgatgttgc
     7681 aacgcagcat ttgcgcgatt tacatcaact tcttgcccgt tgataaacgc ccgcaaagat
     7741 ggggttaccg gcaatggcac ttttcggtca gactcatatt ctgcacgatt gcgcgacagc
     7801 ggctcatgaa cttccagcca ctttgaacca tccggttccg ttgtgtattt cactggctga
     7861 tcaataattt gtacacgagt gcctacagga acattatcaa acagatattt gatatcgtca
     7921 ttacgcagac gaatacagcc ctgacttaca cggagcccaa taccaaaatt ggcattggta
     7981 ccgtggatgg catacaacct gccaatataa atcgcgtaca gccccatggg gttatcgggc
     8041 cccgcaggaa caaatgcggg caaactctcc cctcgtttcg catattcgcg ccggatgttc
     8101 ggcgttggcg tccaggttgg tgcttcttgt ttacgttcaa cggtagtcac ccagttacgc
     8161 ggggtttctc gcccagcctg gccgatacca ataggaaaga cttccactgt attactgtct
     8221 ggcggatagt aataaagacg catttcagcg acgttaacca caatcccttt acgaacagtg
     8281 gcgggcaaaa tcagttgctg cggaatggtg agttgtgagc caggcttcgg caaaaaaaca
     8341 tcagcgcccg gattcgcttc cagcatatta cttaaccctt gcccgtattg tgcggcaaaa
     8401 gtctccagcg gctgagtatt gtgatcagga acagttacag taagcgactg ccccactaaa
     8461 cggctaccct ctggaggtaa tggataagtt accgccaggc tattttggct ggcaaaaagc
     8521 agagcaaatg agcaaagaat atttacacga cgcatcatgt ccctttccta agtcgcgaat
     8581 actatccgat aagtatagct tttatcagac ttttcgtttt tagctgttca aatcagaagt
     8641 cgtattcccc ggtagaacaa tattactggc agcaagttcg cccatgttgt tgtatatcgc
     8701 acaagcagct tcgatgatgg acatcgccag agctgcacca ctcccctcac ctaaacgcat
     8761 atccatattg agataaggct ccagccccaa atgcgagagc gctatacgcg cgcctttttc
     8821 tgccgacaag tgagaaggaa tgagatacgg tttgattgca ggagacatct ggcaggctgc
     8881 gagcgcagca gcataagaaa gaaatccatc cagcaacacg ggtaaaccgc aggaagcagc
     8941 acctaacatc actccagcta ttccgaccaa atcaaatcca cccacttttg ccaggacatc
     9001 aacaccatcc tgaggatttg gttgattcaa cgtaatcgcc cgacgcacaa catcaatttt
     9061 attagccagt ttatctgtcg gcaggtttgc gccaatccca accacttctt caggatcccg
     9121 gccagtgatt gtgctgacta ttgccgctgc cggtgtcgtg tttgccatcc ccagttcacc
     9181 tacaccaaac agcgtgacac cgtttttcgc cagctcccgc gtataacata tgacgtccaa
     9241 aagcaacttt tcagcctgac ggcgactcat tgccggagct gaagcaatat tgccgctacc
     9301 tcgtgcgaca cgcatgttga taagcccggg gataggctca gcagtatcaa taccaacatc
     9361 aattacgtgg acgttagcgc ccgcttgtgc tgccagcaca cacacgccgg ttgttccacg
     9421 ggtcatattt tcagcctgta tggctgtcac ttcttttgga gaaatagcga ccccttcctc
     9481 ccagacgccg tgatcggcac acataaccag taccgctttt ttgcccacat gcggtatgcc
     9541 attcaccccc ggcattcctg ccagttgtat ggcaagcgcc tccagctttc ctaggctacc
     9601 aacaggtttg aataacccgt caacatgccg ttgtgcacgc gacatagcgg cagaattgat
     9661 ggctgggatc gtattcagta aatcggcaat tatttgcatg tcacgtttct ttatagcagt
     9721 gccagcagga agaccagttc accaagctct atggctgctc ccagcgtatc gcccgtttgc
     9781 ccgcccagcg tacgtttgag aagctgaccg aggatgaaaa tcgccaccat cgtcaccacc
     9841 atagcagcca caccatgcat acccagcaat aatacggcag caaaaatagc ggctaagccg
     9901 agtgtgacgc aggtttgtcg cccatcaatt ttgccaatga acacattgcc aagcccttct
     9961 tcccgcgcat aacgatgacg atacatcaat aatgcggcag tcccgcggct gaccgcacat
    10021 gctgccgcca gcgatgcaag gatcggttcg ccacgcagag ccaactcgct taacaccaga
    10081 atctttgcca gtaccacaaa aatcaatgcc agaccgccgt gggtgcctaa acggctatca
    10141 cgcatgattt ccaacatgcg atcgcgacta cgtgcagaaa atacgccatc acaggtatca
    10201 gcgaggccgt ccagatggaa ttccccggtc atcagcgcca gcacaagtac gctaaacagt
    10261 gccgccagtg gtgcgccaca ccatgcctgc agcaccatga agaccagccc gctaatcgcg
    10321 ccaagcaata atccaatcaa aggaaaagta ataataccgc gagaataatg ttcgaaatcc
    10381 agtccctggg accagcgacg cggtacaggc agacgcgtaa taaatgagag catcgcccaa
    10441 aataatttgc tcatttgatt tttactccaa tacccgaaac caccagccat acttcatttg
    10501 ccgcagcggc caactgctga tttacccgcc cggcaatatc acgaaaatgt cgtgccagac
    10561 gactctccgg cacaatcccc attcccactt cgttagtcac taatacaacc tttgcggggc
    10621 aacgttggca ggcagcaatc aacgactgaa tctcagcatt aatcgcctgt tccatcgcct
    10681 gataatccca ttcatcaggg tctttatcgc cgccataatc aaacaacaga ttagtcacca
    10741 ttgtggtaac gcattccagc aacacagcct cattcgggtt aatgtctgca tgaattaatt
    10801 catcaagatg ttgccagcgc tccactgtgc gccagtgctc cgggcggctt tgccgatgat
    10861 gttctatccg tgcagccatc tcatcat
//
LOCUS       DAJTQQ010000116        10865 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000116 DAJTQQ010000000
VERSION     DAJTQQ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10865
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.116"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(50..1264)
                     /gene="rspA"
                     /locus_tag="OO309_003447"
     CDS             complement(50..1264)
                     /gene="rspA"
                     /locus_tag="OO309_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR5848460.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1470..1796)
                     /locus_tag="OO309_003448"
     CDS             complement(1470..1796)
                     /locus_tag="OO309_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCR5848461.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1931..2272
                     /locus_tag="OO309_003449"
     CDS             1931..2272
                     /locus_tag="OO309_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR5848462.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2338..2867
                     /gene="speG"
                     /locus_tag="OO309_003450"
                     /pseudo
     CDS             2338..2867
                     /gene="speG"
                     /locus_tag="OO309_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2870..3580)
                     /locus_tag="OO309_003451"
     CDS             complement(2870..3580)
                     /locus_tag="OO309_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR5848463.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3688..3993
                     /locus_tag="OO309_003452"
     CDS             3688..3993
                     /locus_tag="OO309_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR5848464.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4192..4512
                     /locus_tag="OO309_003453"
     CDS             4192..4512
                     /locus_tag="OO309_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase"
                     /protein_id="HCR5848465.1"
                     /translation="MSKNDRMVGISRRTLVKSTATGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQGTRLFARSLH
                     PPAY"
     gene            5072..5650
                     /locus_tag="OO309_003454"
                     /pseudo
     CDS             5072..5650
                     /locus_tag="OO309_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(6336..7325)
                     /locus_tag="OO309_003455"
     CDS             complement(6336..7325)
                     /locus_tag="OO309_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848466.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(7410..7901)
                     /locus_tag="OO309_003456"
     CDS             complement(7410..7901)
                     /locus_tag="OO309_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848467.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYGRRQMNKRAEAERIAKEQRLQAE
                     RMREENMTDAEWQKELQRREQVKAERRTYGESLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            complement(8222..8722)
                     /locus_tag="OO309_003457"
     CDS             complement(8222..8722)
                     /locus_tag="OO309_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848468.1"
                     /translation="MTSDNKQEYTIHIFGQDITSQDGLFRLDEIRRVGIQIGKVEDNR
                     STEVARFMRTKAGRSLTNPENGFVKKINMGHLGTSWLADPVAAIEFGRWLDLGYGLAV
                     TRAFVSMTSSATVQSAVASAEDIKPETKEFIQRHGTGLYIAGREVSQAEYSDYQAVTA
                     GRCEWR"
     gene            complement(8712..9227)
                     /locus_tag="OO309_003458"
     CDS             complement(8712..9227)
                     /locus_tag="OO309_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848469.1"
                     /translation="MNTELPVTNRQFLIILLTNAESSVDCCRYNLDLLKLSRWYAQAE
                     DWDYEKIREMYAEPIQSYLQTHTEEELKRGLQDIFYFNNPYTCEGDSEEMDWIASRSD
                     ADLKHISNAMMQVESKWSRRLQVLTEDYENGTFDKLVPPDAERLQAKADQEQRFKSFA
                     EALAGGASYDF"
     gene            complement(9658..10026)
                     /locus_tag="OO309_003459"
     CDS             complement(9658..10026)
                     /locus_tag="OO309_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848470.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
     gene            complement(10438..>10865)
                     /locus_tag="OO309_003460"
     CDS             complement(10438..>10865)
                     /locus_tag="OO309_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5848471.1"
                     /translation="VTLDNGLVCIDINEDGDGKSVKNKHSVRLVPLTDGAYGFDLTSF
                     LSWVDMQPDEGPLFGMTPSAYSSWFNSRVLTEALGDSQDVSLHSLRHWLATRMKERGV
                     NLVDAQGILGHSSQSITYDLYGKGHAVGRLADVLKTALL"
BASE COUNT         2791 a         2757 c         2679 g         2638 t
ORIGIN      
        1 tgactctgag gtgtctgtta aacccgtggc gattcaatta tcttactcct tacctgttcc
       61 acagcgtgcc atcttccaga cgtgcgactg gtagataagc aggttcatag ggatatttcg
      121 ccgccagctt ttcatcgaat tcgataccaa gacccggttt gtctcccgga tgcatatagc
      181 cgttatcgaa agtccagttg tgcgggaaga cttcgagcat ttgttcggaa taacccatgt
      241 attcctggac accgaaattg gggacccaca gatcaaagtg cagcgccgca gccatgcaga
      301 ctggtgacaa atcggaagga ccgtgtgagc cagtacgtac ctgatacagc gaagcaaaat
      361 cggcaatccg gcgcataccg gtaattccgc ctgcatgggt cagcgtggtg cggatataat
      421 cgatgagttg ctcttcaatc agttgtttgc agtcccagat gctgttgaag acttcaccca
      481 ctgcgatggg tgtgacggta tgttggcgaa tgagacggaa gcattcttgg ttttccgctg
      541 gcgtcgggtc ttccatccag aacatgcgat aatcttcaat gcttttacca aagcgcgccg
      601 cttcaatagg cgttaagcga tggtgcatgt catgcagcaa atgttcatta aaaccaaact
      661 tgttacgtac cgcgtcaaac aatttcggca tgaaatcgag gtatttctcc gtcgaccaca
      721 gctgctcttc cggccactgt cctttggttg cgggttcata agccagacct ttacctttcg
      781 acatgccgta ggtggttttc ataccaggga ttccgcactg cacgcggatg gctttgaatc
      841 ccagctcctg atgacgggca taatcatcca gagcttcatc aatactgtga ccggtggtat
      901 ggcaataaac catcacccct tcacgagacg cgccgccgag taactggtaa agcggcatgt
      961 tggcagcttt ggctttaata tcccacagcg ccatatcaac cgctgaaatg gccgacatcg
     1021 taaccggacc gcgacgccag taagcacctt tatagaaaaa ctgccagata tcttcgatac
     1081 ggtgcgcatc gcgaccaata agccgcggac aaagatgatc ctgcaaataa gaggccacgg
     1141 aaagctcacg tccattgagg gtggcatccc caaggcccgt aataccgtcc tcagtggtga
     1201 tttttaatgt gacgaaatta cgccccggac aggtaacaaa aacttcagcc tttacgatct
     1261 tcatgttcga ttccttgcat cgcttgtcgt gatgcatgaa atctacgcaa ctgagctact
     1321 gccatacaag tatgaagatc gaaaaaagcc ggagtgatca caaaaaaagg cgtatatttg
     1381 cgctgtgaat ggttgacaaa agatgaaata gaataccttt tgtcagctga cacttcctcg
     1441 tatcttattg ataaaatgga tttatgttcc tacgtgcgcc cccagcccgc aacaatgatc
     1501 aacattccgc aaagcgcaat caacgcaccc gtccagtcat aaagacttag tttcacgcca
     1561 tccacaacgc gcagccacat caatgccgtg cagacataaa cgccaccata agccgcgtaa
     1621 acacgcccac tcgccgcagg atgcaacgtt aacaaccaga caaacagcgc cagtgaaatc
     1681 cccgccggaa gcaacagcca gatactggcg tttcgtttta accacaacca gggcagaaag
     1741 catccaataa tttcacacag cgcagtagca aaaaatagta acgttgtttt aatcatcttt
     1801 gtcacttatt gacatcatgt atagttatag ggcgacataa tatcatcaat ataaacatcc
     1861 tcctggtacg ttttgcgttc gcagtggatg gtgtagaatc acctttaatc attcatacag
     1921 ggaatgaatt atgaaaatca ctctcagcaa acgaatcggc ctgctcgctt ttctgctgcc
     1981 ttgcgcactg gcattgagca caactgttca tgccgaaact aacaaactgg tgattgagtc
     2041 tggcgacagt gcacaaagcc gccagcacgc cgctatggaa aaagagcaat ggaatgacac
     2101 gcgcaatctg cgccagaaag tgaataaacg cactgaaaaa gagtgggata aagccgacgc
     2161 cgcttttgat aaccgcgata aatgtgagca aagtgccaac atcaatgcct actgggagcc
     2221 caatactttg cgctgcctgg accgtcgaac tggccgcgtt attaccccct aacctgttat
     2281 tgatttaagg aatgtaagga cacgttatgc caagcgccca cagttaagct acgcccgctg
     2341 gagcgtgaag atttacgcta tgtacatcaa ctcgacaata acgccagtgt gatgcgttac
     2401 tggtttgagg aaccctacga agcctttgtt gaactctctg atctgtatga taagcgcata
     2461 ttcacgatca gagcgaacgg cgctttgtgg tggaatgtga cggcgaaaaa gccggtctgg
     2521 tggagctggt ggaaattaac catgttcatc gccgggcaga atttcagata attatctccc
     2581 cggagtatca ggggaaaggt ctggcaaccc gtgccgccaa attagcaatg gactatggct
     2641 tcaccgttct caatctctat aagctgtatc tgatcgtcga taaagagaat gaaaaagcga
     2701 ttcacattta ccgcaagctt ggctttacgg ttgaaggtga attgatgcat gagtttttta
     2761 ttaatggtca atatcgtaat gccattcgca tgtgtatatt ccagcatcag tatctggcag
     2821 agcacaaaac accgggtcag actctcctga agccgaccgc acaatagcat taataataat
     2881 cgatcgtatt tttgatggtg taaacccgtt cgacggcggg ttttactcct tcatcaacaa
     2941 tgatcagctg acagtccact ggattggcgt gactgtcata ttcacagctc tgttttacat
     3001 taccaactcg ttgattattt agtaaagtaa ccgctgtgta atctaatttt ttgatcggat
     3061 ccgttgatgg cgtggcgctg acagataatg ttttgtcgtt acttttcgtg gttttaccca
     3121 gcggataccc ctgatcatca tagcgatatt ccatctgcat ttgtttgctg ctggctttaa
     3181 tcacgaagcc attatcatcg gtttcccaac tcaccccggc agaaggtaat tctgctagct
     3241 ggcattttcc ctgtaaacgt actctcttct ccagcgtctc ggcatcacgg taataattgg
     3301 cgtccagtac cagagcgacc acggtattat tttccagatc cagtaattcg agtgaatcaa
     3361 aacagccttc ttccgacaaa gtcccagaaa cacgtttcgt cacttcacct tgctcatcca
     3421 ttaatgtctg agtgaaatct tttaccggac cacgcagcgg atcaaaatcg aattcattag
     3481 agaaactggc catctcaggg gtaaatgaaa gtgttacctc tgtgcggtca catcctgtga
     3541 ggaatatcgc gagtaagcat ggtagtaatt tgtatttcac aacagtcacc agagagtaga
     3601 gatgattctc aatcatagta gcaaatacag tactttacac gttaaatgct atgcttaaag
     3661 aagttatctt cgcgtaagga gcttatgatg aaactctcaa cctgctgtgc cgcgcttctg
     3721 ctcgccctcg cctcacccgc ggtactcgcc gcgccgggat cttgtgagcg catacagagc
     3781 gatatatcac agcgcattat caataatggc gtacccgaaa gcagcttcac gttaagtatt
     3841 gtacccaatg accaggttga tcagcctgat tcccaggtcg tcgggcattg cgctaatgat
     3901 acgcataaaa ttctctacaa ccgcaccacc agcggtaacg tctctgctcc cgcgcagtct
     3961 acccaggatg gcgcgcctgc cgaaccgcag taacacatta tcgactgaac gccggatatg
     4021 acacatccgg cgatttgaac atacaacata atctcacctt attactcata cccttctatt
     4081 gatgtggatt aataattctt aacccaaaat gggtagactc cctctattgt tagcgcgcta
     4141 aatattcaat gtataaacat ttatataaca ataaagaaca gggagtgagt tatgtccaaa
     4201 aatgaccgaa tggtgggcat cagccgcaga acactcgtta aatcaaccgc gacaggttct
     4261 ctggcgctgg ctgcaggcgg tttttcttta ccgttcaccc tgcgcagtgc agcggcagcg
     4321 gtacaacagg ccagcgaaaa agtgatctgg ggttcctgtt ccgtcaactg tggtagccgc
     4381 tgtgcacttc gtctacatgt taaagataat gaagtgacct gggtggaaac tgacaatacc
     4441 ggcagcgatg agtacggcaa ccatcagggt acgcgcctgt ttgcgcggtc gctccatccg
     4501 ccggcgtatt aatcatcccg atcgcttgaa tgacccaatg aaacgcgtgg gtaaacgcgg
     4561 tgaaggcaaa ttcgaacgga ttagctggga tgaagccctg gatactatcg ccagtagcct
     4621 gaaaaaaacc gtcgaacaat atggcaatga agctgtatat attcagtact cttcggggat
     4681 tgttggcggc aatatgaccc gctcttcgcc atcagcctcg gcggtcaaac gcctgatgaa
     4741 ctgctacggc ggttcactca atcagtatgg ctcctacagc accgcgcaaa tttcctgtgc
     4801 catgccctac acctacggca gtaatgatgg caacagcacc acggatattg aaaacagcaa
     4861 gctggtcgtg atgtttggta acaacccggc agaaacccgc atgagcggtg gtggcatcac
     4921 ttatcttctt gaaaaagcgc gcgagaaatc gaacgccaaa atgattgtta tcgatccgcg
     4981 atataccgat acggctgccg gtcgtgaaga ggaatggctc cctattcgcc cgggcaccga
     5041 tgccgcgctg gttgcgggta ttgcctgggt attgattaac gaaaatctcg ttgatcaacc
     5101 ttttctcgat aaatactgcg tcggctatga cgaaaaaacc ttacccgcag atgcacccaa
     5161 aaatggtcac tataaagcct atattcttgg tgaaggtgac gataaaacag caaaaacgcc
     5221 gcagtgggct tcgcaaatta ccggtatccc ggtagaccgt atcatcaaac tggcgcgtga
     5281 aattggcaca acaaaacccg cttatatctg ctagggctgg gggccgcaac gccaggcaaa
     5341 cggcgagctg actgcacgcg ctattgctat gttacctatt ttgacgggca atgtcggcat
     5401 cagcggcgga aatagtggcg cgcgtgaatc gacctatacc attacgatag aacgcctacc
     5461 ggtgctggat aatccggtca aaacgtcaat ctcctgcttc agctggacgg atgctatcga
     5521 tcatggcccg caaatgacgg cactccgcga cggtgtccgg ggcaaagata aactggatgt
     5581 gcccattaag ttcatctgga actacaagga cttgaagcgg acccttagta ggcctgaggt
     5641 cctgcattga gtcaagacct tgtgtcattc tttgtgccag acttgtgtca ttagacggga
     5701 tgtctagacg gctggtcagg accctccggt ggcctcggtc tggacttgag gcgggctatg
     5761 ggggaagtct gctggcttaa tgtattagat ggtcattcac atttttctgt cagaaaccat
     5821 cggtcccact gtgagtacaa acctaagccc ccagaagccc acccacagcc ttagacagag
     5881 tgtcgaatac attcacaccg agtcgaccgg agaggaccat atagaggccc acagagagga
     5941 acacgagttt atagaccaga gtcaggaggg accttaggtc ttcggaagtc atggacataa
     6001 agcccccccc tatcttctat tgcactaccg cactagacaa ctagtacaaa acaaaggccc
     6061 agaccattgg tccagaccgt agttagttaa tcacagtgac aagccaccac ctgaagtcct
     6121 gctgatattc agtccagact tacctctgct catggtctta cctccctcgg tctcctgacc
     6181 ttgggtatcg actacagctt gctgagcgtc atctggagga gctacaggag gagctacagg
     6241 aggcgggacc gaagccccac cttgagtccc accaccacct gtcaggccct tggcgatgga
     6301 cctgaagcct ttactaatgc tcttacccat tggtcttacg cttcggtctt ggtgaacttc
     6361 aggcgtttga ctacaggcag gacggcagta gctgagccag tgcccatctt gatggtcgta
     6421 gcagcttcag cgacaacgaa cactttaccg aggatttcca agtcgtggga ctgagagtcg
     6481 ccatcacgtg aaaggacgac cgtaatattc tcaccgtcgt gacggaagca gccgaagctc
     6541 agggccgtga agcgtttagc cagcatgaat acacccttgt cttcaccgtt gtaataggcg
     6601 atgtctgtcc ccagcatatc gacgatagcg tcttcaccgg agaagccttg cttagcagcc
     6661 agacgggaca gtgctttcag ttggctctca tgagcgataa taccgtagtc agacacctca
     6721 gaaccgaaag aagactctgt gttctcatac agagcgtcca tgatagcgtc tgccttagcg
     6781 gtcaggctac cagtcaggac aggaagctct acggtcttca gcagcttaga ggattccaga
     6841 acgtcgacca tgcgagcgtt gatggtacgg accacattct cacagatgtt catagcgtcc
     6901 tgctgaagtg tgccctgagt gaatccggtg gatgacttgg cgtcccagag cttggtctgc
     6961 tcagagatga gaatagcctg ctcgattggt tcgaccacga tttcttgtcc agacggaacg
     7021 gtcacgactt caggtcgctt gccaccacgg acaggaacga ccacagagcg gtcagccaga
     7081 gtgtgagcaa tgattggggt cggtaagctg ttacgggtcg gcaggacttc tacagggaac
     7141 gggatgttct cttgagcgtc tttaagtaca ccgttcagca tagccttaac cgtcttagct
     7201 aactggaggg catagacctg cgggtcagcg ttagtcggca gaccttcatt gacctgaatg
     7261 ttgtcggtat cggtgatgcg tactggctca atgccaaact ggaaagcgtc tttaatcgta
     7321 gtcataataa tcaatctcct aaattagttt aaatactgtg agtcctcaaa actgaggagt
     7381 ccctaacatc gggtcaggga ctgtgtgttt tacaagctgt ccatccagtt accgcctgac
     7441 tcaaggtcag cgacaatagc ggcacgggag cgaccagcgg aatgggtagc actgcggagg
     7501 ctctcaccat aggtccggcg ttcagctttg acctgttcgc ggcgctgtag ctctttctgc
     7561 cactctgcat cggtcatgtt ctcttcacgc atacgctcag cctgaagtcg ttgctcttta
     7621 gcgatacgct cagcttcagc tcgtttgttc atctggcggc gaccataggc ggtcatcaag
     7681 tcattactga agaagttgcc aatggtcacg ccagcttcag ccagagcttc cagtaactcc
     7741 attgcctcgc ggctggtaga cggcaggcct gacaggtagc ccggaatgag ggtcttgata
     7801 gcgagcgaca gcggataggg ctgaccgtcc agactcacag tgttttcata aaggttgatg
     7861 tcggtgactg acagcttcca gttgttgttg ttgaacatca taggtaattc tcctcttgag
     7921 agttgagact atcggtatcg cagacgcagt agcgcctacg ggtctccccg catcggtcat
     7981 ctcaaaaggt ttaaaattag gtttggtatg gtcaggactg cttagcaaag gctcggcgta
     8041 actgattgtc taaatcacgg tccaccagca tagctatcag ctcgtctcga ctgtagtaag
     8101 ggcgctcagg gtcttcgact ccaagtctca gcagagacag cagatgacgt gtgggcaggc
     8161 cacggtaacg aagggtcgct ttgccgatag gtgtacgagg gtcaatcata gccatgatta
     8221 cttacctcca ctcacagcga ccagcggtca cagcttgata gtctgaatac tcagcctgtg
     8281 agacttcacg tccggcgatg tagaggcctg tgccatgacg ctggatgaat tccttggtct
     8341 ccggcttgat gtcctcagca gacgctacag cagactgcac ggtggcgctg gaggtcatag
     8401 acacgaaggc tcgggttact gccagtccgt acccaaggtc caaccaccga ccgaactcta
     8461 tagcggctac tgggtcagcc agccacgagg tccctaagtg acccatgtta atctttttaa
     8521 caaacccgtt ttctgggttg gttagacttc taccagcctt ggtcctcatg aagcgagcaa
     8581 cctcggtact acggttatct tctaccttac caatctgaat gcctacccgg cgtatctcat
     8641 ccagtcgaaa caatccgtcc tgactggtga tgtcctgacc gaagatatga atggtgtatt
     8701 cctgcttatt gtcagaagtc ataagaggca cctcccgcca gagcttcggc aaaggacttg
     8761 aagcgctgtt cttggtcagc tttcgcctga aggcgttcgg catcaggtgg gaccagctta
     8821 tcaaaggtgc cgttttcgta atcttcagtt aggacctgaa ggcggcggga ccatttgctc
     8881 tccacctgca tcatcgcgtt agagatgtgc ttcaagtcgg catcagagcg tgacgctatc
     8941 caatccatct cttctgagtc accctcacag gtgtaaggat tgttgaaata gaagatgtcc
     9001 tgcaggccac gcttcagctc ttcctcggtg tgtgtctgga ggtatgactg gataggctcc
     9061 gcgtacatct cccggatttt ctcatagtcc cagtcttccg cctgggcata ccatcgggat
     9121 aacttcagca ggtctaagtt atagcgacag caatcgacac tggactctgc attggtcagc
     9181 aggatgatta agaactggcg gttagttact ggtaattcgg tattcataca aagatgctcc
     9241 tttggtaaag cactttctgt ctccacatgg agtccgcacc attcagacgc atcctaaacg
     9301 gacccatcag aaagtgctta acgaaagtga atggttaaat gttgacggtc tggagtgacc
     9361 atgttctcca tgtacgtata aaggtgcgac cgcatgtcag gacgagctgt aaggactcca
     9421 gatggtgagc ggtgatagac ttaagccaat gtagtgagta gacgagctac taaccgagac
     9481 gactaaagaa cgctaacgag ctggaggtcg atacaggagg tctgtcagag gcggtcacgg
     9541 gccttgatta cttaggagag atacttaatg tcggttgacc agatgctcgc atgttctgac
     9601 gcatcagact cagaggcgga ggctggtctc cctatagtga tagtttaggg tccgtaatca
     9661 tgaaccagct ttactggcgt ggtactggac ggatttactg gcgctgacga cggtagcata
     9721 gacgtagcga gcgtagcgag ttgaaaccat aggttcaccc aaggcaacac gcttacggct
     9781 cagggactcc tgcacagcgc ttacagttaa tgtgtcgtgc gttagtagtt gggtcagaat
     9841 caagtatggg ctgacaggct tggtattgct tgccattgat tcgttcaggc gaccagcacc
     9901 agtaaggaca tcatccagcc aaccactcag gtcgccctga ccatcgaatg acagcaggtc
     9961 cccgcagtgc tgctgcaagt aggctttggt gaacgtgttg tcgtgagttg cgattggtcg
    10021 tgccatagag tatctcctgt agttgaatta gttggttccc ctatagtgat aatcaagtcc
    10081 atcactggtg atgcatacag tagcgcgaca ggatgtgact cacaggttga accgaatgtc
    10141 aaggactaaa gacgcctcag gaattaagga agtatatata aagagagacc agaggcgact
    10201 cacagttaaa caggaatgtc aagaggagac cataggaatt tggagggact ggagtttagg
    10261 acgcagttta aataagacca cagacagact tagagtcgga cagccgaagg cggaccagtg
    10321 ggcctatagt caggagggat tatgattatt atcaccctcc ctaacttcag tcagacttca
    10381 gtgacccaag gtctcaacca tcaagccgta tatggtctcc accttgggtc tccctactta
    10441 aagtaaagct gtcttaagca catcagccag cctacccaca gcgtgtcctt tcccgtacag
    10501 gtcatacgta attgactgac tgctgtgacc taagatgcct tgagcgtcca ccagattgac
    10561 tccacgctct ttcattcggg tcgccagcca atgtctcaga ctgtggagag agacatcctg
    10621 agagtctcct agggcctcag tcaggaccct tgagttaaac cagctagagt aggcgctcgg
    10681 agtcatcccg aagagaggac cctcatcagg ctgcatgtct acccacgaca ggaaggacgt
    10741 caggtcaaac ccataagcgc catcggttaa cggcacaagt cggactgaat gtttgttctt
    10801 cacagacttc ccgtccccat cttcattgat gtcgatacag accagtccat tgtccaaggt
    10861 tacaa
//
LOCUS       DAJTQQ010000117        10835 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000117 DAJTQQ010000000
VERSION     DAJTQQ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10835
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.117"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..285
                     /locus_tag="OO309_003461"
     CDS             <1..285
                     /locus_tag="OO309_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5848472.1"
                     /translation="AVAVWLSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWM
                     ENTIFPAMKDIPVLSGLIDTMIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            296..1036
                     /locus_tag="OO309_003462"
     CDS             296..1036
                     /locus_tag="OO309_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR5848473.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1095..1481
                     /locus_tag="OO309_003463"
     CDS             1095..1481
                     /locus_tag="OO309_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR5848474.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1490..1819
                     /locus_tag="OO309_003464"
     CDS             1490..1819
                     /locus_tag="OO309_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR5848475.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            1791..4832
                     /locus_tag="OO309_003465"
     CDS             1791..4832
                     /locus_tag="OO309_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5848476.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            4832..5161
                     /locus_tag="OO309_003466"
     CDS             4832..5161
                     /locus_tag="OO309_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5848477.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            5161..5859
                     /locus_tag="OO309_003467"
     CDS             5161..5859
                     /locus_tag="OO309_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5848478.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            5864..6607
                     /locus_tag="OO309_003468"
     CDS             5864..6607
                     /locus_tag="OO309_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5848479.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6544..7146
                     /locus_tag="OO309_003469"
     CDS             6544..7146
                     /locus_tag="OO309_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR5848480.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            7207..10686
                     /locus_tag="OO309_003470"
     CDS             7207..10686
                     /locus_tag="OO309_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5848481.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            10754..>10835
                     /locus_tag="OO309_003471"
     CDS             10754..>10835
                     /locus_tag="OO309_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5848482.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEH"
BASE COUNT         2549 a         2566 c         3472 g         2248 t
ORIGIN      
        1 gccgtggcgg tgtggctgag tgatgcgcag tacaccggaa aaatgacgga tgaggacgac
       61 tggcaggctg ttctgcatat tgccgttttc gtccgtgcac aggcgccgga ttctgagctg
      121 gatacgtgga tggagaacac catatttccg gccatgaagg atattccggt gctttccgga
      181 ctcatcgaca ccatgatccc actcggtttt aactatcaac gtgataatga gatggccacc
      241 tgggcgatgg cggaaatcac gtaccagatc acgtacacga attaaggagg tggtaatgac
      301 cacaccaaat ccactggcaa agacgaaagg tgcggggacg acgttctgga tgtataccgg
      361 caacggcgat gcgtttgcga accctttgtc ggacactgac tggctgcgtc ttgcgatggt
      421 gaaggatctg caacctggcg aaatgaccgc tgatgcagaa gatgacactt atctcgatga
      481 tgaagatgca gactggaaaa cgacaaccca ggggcagaaa tccgttggtg atacttcggc
      541 gacgctggcc tggcgtccgg gtgacagcgg gcagaaaaaa ctggttcagt tgttcgactc
      601 cggtgaagtc tgcgcgtttc gtatcaaata tcccaacggc actgttgatg ttttccgcgg
      661 ctggctgagc tcactgggta aaaccattgc ctcaaaagac gtgatgaccc gcactgtgaa
      721 aatcagcggt gtggggcgtc cgtatctggc agaggaaggc actgaaaccg tgggtgttac
      781 cggactgacg gtggcaccgg catctgtcag tgtcaaagcg ggagcaacca ccacactgac
      841 ctttacagta aaacctgacg gggccagtga caaagcgatc agtgtgcatt cgtcagatcc
      901 tcagactgct tcggtgaccc tgagcgggct tgtggccacg gtgaaaggcg tgaagcaggg
      961 cagtgtcagc atcgtgggca tgacctctga cggagagttt gtggcagtgg ctgcggtgac
     1021 cgtcagcgca ccataacagg acgatactca tcattgcccc ggttatccgg ggctttttgc
     1081 atccggagaa catgatgttt ctgaaacagg atacgtttaa ttatgaaaaa cagtccgtgg
     1141 tgctcagtga gctgtccggg ctgcagagaa ttgaatatct gacgtttgtt cagcagcgaa
     1201 cggcaaagtt tgatgcacag gagggagaac tgccggaggc tgaacgacag attgcttttc
     1261 tgcgtatggg aatggatatc aatgcctggc tggtttcccg ctcactgtgg aatgctgagc
     1321 agtctcagga tgttgagacg ctttgcgcat ccattatgac aacatggtcg tatgatgcgc
     1381 tgggcgcggg ggcggagagg gttctgtcgc tgagcggtat ggggaccatt gagaatgccg
     1441 gggatgatga tcatgaggcg ctgacgccgg aaaagtcctg acgcgggaaa tgcagtttgt
     1501 catgcggctt gcccgggagt tccggcgggc agactggcgg cggatgctgt cggatatgtc
     1561 ggccactgag cttggtgagt ggggcgatta tttccggatg cagagcttca gtgatgtgtg
     1621 gatggatgcg cagtttgcct cgctgaaggc attgatcgtg agaatggtgt ccggcagcag
     1681 cgatgctgcg gtggctgatt tcagcctttt accggaagag aacgggatac cggagcgaac
     1741 ggacgaagaa ctgatgcatc ttggggaagg tatttccgga ggtgtgcgtt atggaccaga
     1801 tagccaacct ggtcattgat ttggggattg atgcggcaga gtttaaaaat gaaattcccc
     1861 gtatcaaaaa ccttctgaat ggtgcagcca gcgatgcaga acggtcttct gcccgtatgc
     1921 agcgttttat ggagcgtcag actcaggcgg cccggcagac aatgcaggcg gcttcttcgg
     1981 ctgcaacagc agcatcagcc catgcgcaga cggtggagaa gaatgcacgg gctcatgaac
     2041 gcatggcccg tgaggtggaa caaacccgtc tgcgcgtgga tgccctgaat cagaaaatgc
     2101 gcgaggaaca ggcgcaggcc agggcactgg cggaggcgca ggataaagcg gctgccgcct
     2161 tttatcgcca gattgacagt gtgaaacagg ccggtgcggg gcttcaggaa ttacagcgta
     2221 ttcagcagca gatccgacag gccagaaaca gtggcggggt tggtcagcag gattatctgg
     2281 cgctgatttc ggagatcacg gcgaaaaccc gtgccctgac gcaggcagag gaacaggcca
     2341 cccggcagaa agcggcgttt atccgccagc ttaaagagca ggcaacccgc cagaatctgt
     2401 cgtcttctga gttgcttcgc gccagggcgg ctcagctggg ggtaagcagt gctgcagaag
     2461 tgtatatccg caaaatggag cgggcaggaa aagccacaca ttcgctgggg ctgaaaagtg
     2521 cggcagcccg gcgggagctg ggggtgttaa tcagtcagat ggcgcgaggc aattttggtg
     2581 cgctgagggg atccgggata acgctggcta accgtgccgg atggacaggc gcactgatgt
     2641 cgccgaaagg catgatgact ggcggcgtta ttggcggact tgtcgcggcg gtcctgggtc
     2701 tgggtaaagc ctggcatgac ggccggaagg agggcgagaa atttaaccgt cagctggcgc
     2761 tgacgggaca ttatgccggt gtcactgtcg ggcagttgtg gaaactcagc cgggccatat
     2821 ccgggaatgg tatcacgcaa catgcggcag ccggtgcgct ggcacaggtg gtgggcagtg
     2881 gggcatttca tggaaacgat attggtatgg tggcgaaagc tgccgcacag atggagcgat
     2941 cggttggcca gtcggtcagc gataccataa atcagtttaa gcggctgaag gatgatcctg
     3001 taaatgccgc gaaggcgctg gacaatgcgc tgcatttttt gaatgccact cagcttgagc
     3061 agatacgcgt ccttggggag caggggcggt ccagtgatgc ggcccggatc gccatgtctg
     3121 cgctggcaga ggaaaccggt aaacgcacat ctgatattga taataatctc aatgcgctgg
     3181 gtagtacgct gcaaaccttg tctgactggt ggaagcagtt ctgggatgcg gccatgaata
     3241 ttggtcgtga agactcgctg gatgcgcaga ttgatgcgtt acaggaaaaa attcagcgcg
     3301 cgaaaaaata tccgtggaca aacgcctcca cacaggtgga gtacgatcag cagcgtctta
     3361 acgatcttca ggagaaaaaa cgccggaagg atttgcagga tgcaaaagcg caggcagaac
     3421 ggaattacca ggagcaacag aaacgccgga atgctgaaaa tgccgcgctg aaccggatga
     3481 atgaaacgga agctgcacga catcagcggg aaattgcgcg tattaatgcc atgcagtacg
     3541 ccgaccaggc tgtcagggat gcggcgatac agcgtgaaaa tgaacgttac gaaaaagcca
     3601 ttaagaaaaa tacacgggca acccgtaatg atgaggccac ccggttattg ctgcagtaca
     3661 gtcagcagca ggcacaggtg gaagggcaga ttgctgctgc cagacagtca gcaggcattg
     3721 ccactgaaag gatgactgaa gcgcataaac agcttctggc cctgcagcag cgcatcagcg
     3781 acctggacgg gaaaaagctg acggcagatg aaaagagtgt gctggcccgt aagaatgagc
     3841 tgattcaggc gctgacgctg ctggatgtga aacagcagga gctgcagaaa cagacagcgc
     3901 ttaacgacct gagaaaaaaa acggttcagc tgaccagcca gctggcagac aaagaacgtg
     3961 cactgcgtga gcagcacaat ctggatattg ccactgcagg tatgggggat aagcagcggc
     4021 agcgctacca ggcacagttg cgcatccggc aggaataccg gcaacagttg caacagcttg
     4081 agaatgacag tcgccagaaa ggcacttacg ggacggagga ctaccggagg gctgaggagg
     4141 tgctgaaggg gagcctgaag cgacaactga atgaaaacaa acgctactgg caggaactgg
     4201 aagtggcgca gggcgactgg aaaaacggtg ccatgcgggc gtttcagaat tttacggcgg
     4261 atgcggataa tgcggcggga acggcagaac agatgtttac agtggcattc agcagtgccg
     4321 gtaatgcact ggcgacattc tgtaccaccg gtaagctgaa ttttaaatcc ttcacctctt
     4381 ccctgttgtc agatatggcc agaattatgg cacagatggc catgatgcag gcggtaaagg
     4441 gcgtcggttc tttattcggc ttcacgacta atgctgatgg cggtgtttac cagtctgctg
     4501 atttgagtcg ctacagtggc acggtggtta accgtccgac gttttttgct tttgcaaaag
     4561 gcgcgggtgt gatgggggaa gcgggacctg aagccattct gccactgcgt cgcggtgctg
     4621 atggtaagct gggggttgtg gcggatactg gtggttcagg catggtgatg tttgccccgc
     4681 agtacaacat tgagatcaat aacgacggca cgaacgggca gataggtccg gctgccctga
     4741 aggtggttta tgacctcggg aaaaaagcgg ccgcggactt tatgcaacag caggcccgtg
     4801 atggtggccg gttaagtgga gcataccggt aatggagacg tttcactgga aagtgcgccc
     4861 ggatatgaat gtggtatcag agccgaaagt ggtgacagtg aagctgggcg atggttatga
     4921 acagcgtcgt gcggcgggac tgaataacca gttgtcgact tacagcgtga cgatacgtgt
     4981 tcgtaaaggt gaacacccat ctttaaaagc ctttctggaa cggcacggtg gcgtccgcgc
     5041 atttcagtgg acgccacctt atgactggaa actgatcagg gtggtttgtc gtaaatggtc
     5101 ggcaagcgtg ggggcgttat gggtgactgt aacggccgat tttgaacagg tggtgaactg
     5161 atgcaggata tccggcagga aacactgaat gaatgcaccc gtgcggagca gtcagcccgg
     5221 gtggagctct gggaaatcga tctgacagag gtcggtggtg agcgttattt tttctgtaat
     5281 gagcagaacg aaaaaggtga gccggtcacc tggcaggggc ggcagtatca ggcatacccc
     5341 attcaggggt cggggtttga actgaacggc aggggctgtg ctgcccgtcc gacactgacg
     5401 gtctctaacc tgcacggcat ggtcaccggg atggcggaag atctgcagag tctggtcggc
     5461 ggaacggtgg tccggcgtaa ggtttacgcc cgttttctgg atgcggtgaa cttcgtcaac
     5521 ggaaacagcg acgccgatcc ggaacaggag gtgatcagcc gctggcgcat cgagcagtgc
     5581 agcgaactga gcgcggtcag tgcctctttt gtgctggcca caccaacgga gacggatggc
     5641 gcggttttcc cggggcgtat catgctggcg aatacctgta tgtggactta ccgttctgat
     5701 gagtgtggtt acacgggcag ggcagtggct gacgagttcg acaaaccaac aacggatatc
     5761 cggaaggaca aatgcagcaa gtgtatgcgc gggtgtgagt tgcgcaacaa taccggtaat
     5821 ttcggcggtt tcctttccat caataaactt tctcagtaaa tccatgacac agacagaatc
     5881 agcgattctg gcgcacgccc ggcgatgtgc gccagcggag tcgtgtggct tcgtggtgag
     5941 aacgccggag ggagacaggt atcttcccag cgagaatatc tccggtgagc cggaggaacg
     6001 gttccggatg gctccggagg actggctgcg ggcacaaatg cagggtgaga ttgtggcact
     6061 ggtccacagt catcccggtg gtctgccctg gctgagtgag gctgaccggc ggctgcaggt
     6121 gcagagtgat ttgccgtggt ggctggtctg ccgggggacg attcacaagt tccgctgtgt
     6181 gccgcatctc accgggcggc gctttgagca cggggtgacg gactgttaca cgctgttccg
     6241 ggatgcttat catctggcgg ggattgagat gccggatttt catcgcgggg atgactggtg
     6301 gcgtcacggt cagaatctct atctggataa tctggaggcc acagggctgt atcaggtgcc
     6361 gttgtcatca gcacaaccgg gcgatgtgct gctgtgctgt tttggttcat cggtgccgaa
     6421 tcatgccgcc atttactgcg gcgatggtga gctgctgcac catattcctg aacaactgag
     6481 taaacgagag aggtataccg acaaatggca gcgacgcaca cactccctct ggcgtcaccg
     6541 ggaatggcac gcatctgcct ttacggggat ttgcaacgat ttggccgccg catcgacctt
     6601 cgtgtgaaaa cgggggctga agccatccgc gcactggcca cacagctccc ggcgtttcgt
     6661 cagaaactga gtgacggctg gtatcaggtg cgcattgccg ggcgtgatgc aggtgaaacc
     6721 gaattgtctg cccgtcttaa tgagccgctg gcaaatggtg cagtgatcca catagtaccg
     6781 cgtctggtgg gagctaaaag tggcggtgtg tttcaggcgg tgctgggggc agctgttatg
     6841 gcggttgcta tatggatgcc gggggtagga attatggcga gtaatctgct gttttctctc
     6901 ggtgccagta tgacgcttgg cggtgttgca cagatgctgg caccgaaagc cagaactccc
     6961 cgtacacaga caacggataa cggcaaacag aacacctgtt tctcctcact ggataacatg
     7021 gttgcccagg gcaatgttct gcctgttctg tacggtgaaa tgcgcgtggg gtcgcgggtg
     7081 gtatctcagg agatcagcac ggcagacgaa ggggatggtg gtcaggttgt ggtgattggt
     7141 cgctgatgca aaatgtttca tgtgaaaccg cctgcgggcg gttttgtcgt ttatggagcg
     7201 tgaggaatgg gtaaaggcag cagtaagggg cataccccgc gcgaagcgaa ggacaacctg
     7261 aaatccacgc agttgctgag tgtgattgat gccatcagcg aagggccggt tgacggtccg
     7321 gtggatggat taaaaagcgt gctgctgaac ggtacgccgg tcctggatag cgaggggaag
     7381 acaaactttt ccggtgttac ggtggtgttc cgcgccggcg agcaggagca gacaccgccg
     7441 gaggggtttg aatcttccgg ctcagagact gtgctgggta cggaagtgaa atacgacacg
     7501 ccgatcaccc ggaccatcac gtcggcaaac attgatcgtc tgcgcctgac cttcggtgtg
     7561 caggcactgg tggaaaccac ctcaaagggg gaccggaatc cgtcggaagt ccgcctgctg
     7621 gttcagatac agcgtaacgg tggctgggtg acggaaaaag acatcaccat taagggcaaa
     7681 accacttcgc agtatctggc ctcggtggtg gtgggtaacc tgccgccgcg cccgtttaat
     7741 atccggatgc gcaggatgac accggacagc accacagacc agctgcagaa caaaacgctc
     7801 tggtcgtcgt acaccgaaat catcgatgtg aaacagtgct acccgaacac ggcgctggtc
     7861 ggcgtgcagg tggattcaga gcagttcggt aaccagcagg tgagtcgcaa ttatcatctt
     7921 cgcgggcgca ttctgcaggt gccgtcgaac tataacccgc agacgcggca atacagcggt
     7981 atctgggacg gaacgcttaa gccagcatac agcaacaaca tggcctggtg tctgtgggac
     8041 atgctcactc atccgcgcta cggcatgggg aaacgtcttg gtgcggcaga tgtggacaaa
     8101 tgggcgctgt atgtcatcgg ccagtactgc gaccagtcag tgccggacgg atttggcggc
     8161 acggagccgc gcatcacctg taacgcttac ctgaccacac agcgtaaggc gtgggatgtt
     8221 ctcagtgatt tctgctcggc gatgcgctgt atgccggtat ggaacgggca gacgctgacg
     8281 ttcgtgcagg accgaccgtc ggataaagtg tggacctata accgcagtaa tgtggtgatg
     8341 ccggatgatg gcgcgccgtt ccgctacagc ttcagcgccc tgaaggaccg ccataatgtc
     8401 gttgaggtga actggattga tccggacaac ggtcatgaga cggcgacaga gcttgtggag
     8461 gacacgcagg ccattgtccg ttacggtcgt aacgtcacga agatggatgc ctttggctgt
     8521 accagccggg gacaggcgca ccgcgccggg ctgtggctga ttaaaacgga actgctggaa
     8581 acgcagaccg tggacttcag cgtcggcgca gaagggcttc gccatgtacc gggtgatgtc
     8641 attgaaatct gcgatgatga ttatgccggt atcagcaccg gtggtcgcgt actggcggtg
     8701 aacagccaga cccggacgct gacgctcgac cgtgaaatca cgctgccgtc ctccggtacc
     8761 acgctgataa gcctggttga cggaaatggc aatccggtca gcgtggaggt tcagtccgtc
     8821 accgacggcg tgaaggtgaa agtgagccgg gttcctgacg gcgttgcaga atacagcgtg
     8881 tgggggctga agctgccgac gctgcgccag cgcctgttcc gctgcgtgag tatccgtgag
     8941 aacgacgacg gcacgtatgc catcaccgcc gtgcagcatg tgccggaaaa agaggccatc
     9001 gtggataacg gggcacactt tgacggtgac cggcgcggca cggtgaatgg tgtcacgccg
     9061 ccagcggtgc agcatctgac cgccgaagtc accgcagaca gcggggaata tcaggtgctg
     9121 gcgcgatggg acacgccgaa ggtggtgaag ggtgtgagct tcctgcttcg cctgaccgtg
     9181 gcagcggacg acggcagtga gcggctggtc agcacagcca ggacgacgga aaccacatac
     9241 cgcttcaggc agctggcgct ggggaattac agtctgacag tccgggcggt aaatgcccgg
     9301 gggcagcagg gcgatccggc gtcggtatcg ttccggattg cggcaccggc agcgcctgtc
     9361 actattgaac tgataccggg gtattttcag ataacggtgg tcccgaaact ggctgtatat
     9421 gacccgacgg tgcagtttga gttctggttt tcggaaaagc ggattgcgga tatcaggcag
     9481 gttgaaacca gcgcccgcta tcttggcacg gcgctgtact ggatagccgc cagtatcaat
     9541 atcaggccgg gccatgatta ttatttttac gttcgcagtg tgaacaccgt tggcaaatcg
     9601 gcatttgtgg aggccgtcgg tcgggcgagc gatgatgcgg aaggttatct gagtttttat
     9661 aaagggttga tcaataaaac gcatctcggc aaggagttgt ggacgcagat tgataacggt
     9721 cagcttgcgc cggacctgac tgaaatcagg acgtccatta cgaatgtcag caatgaaatc
     9781 acgcaaaccg tcaataaaaa actggaaaat cagagtgctg caatccagca gatacagaaa
     9841 gttcaggttg atacaaataa taacctgaac agcatgtggg ctgtgaagct gcaacagatg
     9901 aaggacggac gcctttatat tgcgggtatc ggtgccggta ttgagaatac gccagcagga
     9961 atgcagagtc aggtgctgct ggcggcagac aggattgcga tgattaatcc tgcgaatggc
    10021 aacacaaagc cgatgtttgt tggtcagggc gatcagatat tcatgaacga cgtgttcctg
    10081 aaacgcctga cggctccgac cattaccagc ggcggtaatc ctccggcatt ttccctgaca
    10141 cctggcggac ggctgacggc gaaaaatgcc gatatcagcg gtaacgtgaa cgcgaactcc
    10201 gggacgctca acaacgtcac gattaataag aactgtcggg ttctgggaaa attgtccgcg
    10261 aaccagattg aaggcgatct cgttaaaaca gtgggcaaac ctttcccacg ggactcccgg
    10321 gcaccggaga ggtggccatc agggaccatt accgtcaggg tttatgacga tcagccgttt
    10381 gatcggcaaa ttgttattcc cgcggtggcg tttcgcggtg ctaaacatga gcggaagaat
    10441 aacaatattt attcgtcatg ccgcctgata gtgaagaaaa acggtgctga aatttataac
    10501 cgaacgaccc tggataatac gctgatatat acgggtgtta ttgatatgcc tgccggtcac
    10561 ggtcacatga cgctggagtt ttctgtatcg gcatggctgg taaatggctg gtatcccaca
    10621 gcaagtatca gcgatttgct ggttgttgtg atgaagaaat ccacagcagg tatcagtatc
    10681 agctgaattt tataacccag atacgggcac cagaaatggt gcctttttta ttgcagaaaa
    10741 gcgagaggta attatgcgta aactttatgc tgccattttg tccgcagcca tctgtctggc
    10801 tgtatccggt gcgcctgcat gggcgtctga acatc
//
LOCUS       DAJTQQ010000118        10777 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000118 DAJTQQ010000000
VERSION     DAJTQQ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10777
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.118"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            15..365
                     /locus_tag="OO309_003472"
     CDS             15..365
                     /locus_tag="OO309_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR5848483.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            418..813
                     /locus_tag="OO309_003473"
     CDS             418..813
                     /locus_tag="OO309_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR5848484.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            854..1597
                     /gene="cmoA"
                     /locus_tag="OO309_003474"
     CDS             854..1597
                     /gene="cmoA"
                     /locus_tag="OO309_003474"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR5848485.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1594..2565
                     /gene="cmoB"
                     /locus_tag="OO309_003475"
     CDS             1594..2565
                     /gene="cmoB"
                     /locus_tag="OO309_003475"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR5848486.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2730..5159)
                     /gene="torZ"
                     /locus_tag="OO309_003476"
     CDS             complement(2730..5159)
                     /gene="torZ"
                     /locus_tag="OO309_003476"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR5848487.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5184..6284)
                     /locus_tag="OO309_003477"
     CDS             complement(5184..6284)
                     /locus_tag="OO309_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR5848488.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6672..7418)
                     /gene="cutC"
                     /locus_tag="OO309_003478"
     CDS             complement(6672..7418)
                     /gene="cutC"
                     /locus_tag="OO309_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR5848489.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7432..7998)
                     /locus_tag="OO309_003479"
     CDS             complement(7432..7998)
                     /locus_tag="OO309_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5848490.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8214..9947
                     /gene="argS"
                     /locus_tag="OO309_003480"
     CDS             8214..9947
                     /gene="argS"
                     /locus_tag="OO309_003480"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR5848491.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10124..10612
                     /locus_tag="OO309_003481"
     CDS             10124..10612
                     /locus_tag="OO309_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCR5848492.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         2624 a         2750 c         2585 g         2818 t
ORIGIN      
        1 ggtgtattta gaccttgcta atcttagaca gccgcccggt tcatgcatca cgtccacaca
       61 gtgaagctat tcgcgacgct caacgaaaaa aacctaaagt tccggtacat gctgtactga
      121 cggcgacaaa tccgctgatc cgttttatcg gtagtgatga tatgacgcaa aaccgggaat
      181 tatttcaggt ctggttacaa aaattagcgc agtggcatca gaccactacg ccttatcttt
      241 ttttacatac gccagatatt gcccaggccc cggaactggt acataccctg tgggaagact
      301 tacgtaaaac gcttccagag atcggagcag ttccggctat tccacagcaa tcttctcttt
      361 tctgaatttg ccacctatca tagacaggtg ccatcggcca ttttaaaggg agtttgtatg
      421 gtaagcgcgc tatatgccgt tttaagtgcg ttgttattaa tgaagttctc ttttgatgtc
      481 gttcgcctgc gaatgcagta ccgcgttgcc tatggtgacg gtggttttag cgaactgcaa
      541 agcgctattt gcattcatgg taacgcggtg gaatatattc ccatcgcgat tgtgctgatg
      601 ctgtttatgg aaatgaatgg cgcagaaacc tggatggtgc atatttgcgg catcgttttg
      661 cttgctggtc gtctgatgca ttattacggt tttcatcacc gtctgtttcg ctggcgacgt
      721 tccggcatga gcgccacctg gtgtgcgctg ttgctgatgg tgctggcgaa tctttggtat
      781 atgccctggg agttggtttt ctccctgcgt tagcgcacaa tacgccactt tctttttccc
      841 ggatttttac gttatgtctc accgcgacac gctattttct gcccctatcg ccagactggg
      901 cgactggacc tttgatgaac gggtcgctga agtcttcccg gatatgatcc agcgttccgt
      961 tcccggctat tccaatatta tttccatgat tggtatgtta gccgagcgct tcgttcaacc
     1021 tggtacgcag gtttacgatc tgggttgttc tctgggcgcg gcgacgctct cggtgcgtcg
     1081 caacattcat catgataatt gcaaaattat tgccatcgac aactccccgg cgatgattga
     1141 acgctgccgt cgtcatattg acgcctataa agcccctacg ccagtagacg tcattgaagg
     1201 tgatattcgc gatatcgcca ttgaaaacgc atcgatggtg gtgctgaatt ttaccctgca
     1261 attcctggaa ccttccgagc gccaggcatt actggataaa atttatcaag gactgaaccc
     1321 gggcggcgcg ctggtgcttt cggaaaaatt cagtttcgaa gatgccaaag ttggtgaact
     1381 gttgttcaac atgcaccacg actttaaacg tgccaacggt tacagcgaac tggagatcag
     1441 ccagaagcgc agcatgctgg aaaacgtgat gctgaccgat tccgtggaaa cccataaagc
     1501 acgcctgcat aaagccggtt ttgagcatag cgagctgtgg ttccagtgct ttaactttgg
     1561 ttcactggtg gcattaaaag cagaggacgc tgcatgatcg actttggtaa cttttattct
     1621 ctgattgcca aaaatcatct ttcacactgg ctcgaaacgc tgcccgcgca gattgctaac
     1681 tggcagcgcg agcagcagca cgggctgttt aagcagtggt ccaacacggt ggaatttctg
     1741 cctgaaatta aaccgtatcg tctggattta ttgcatagcg taaccgccga aagcgaagag
     1801 ccactgagca ccgggcaaat taaacgcatt gaaacgctta tgcggaacct gatgccatgg
     1861 cgcaaagggc cgttctcact gtatggcgtc aacatcgata ccgaatggcg ttccgactgg
     1921 aaatgggatc gtgttctgcc ccatctttct gatttaaccg gacgcaccat tctggatgtc
     1981 ggctgcggca gcggttatca catgtggcgc atgattggcg caggcgcgca cctcgcggtg
     2041 gggatcgacc ctacgcaact gtttctgtgc cagtttgaag cggtgcgtaa actactgggt
     2101 aacgatcagc gcgcacattt gttaccgtta ggtattgaac agcttccggc actgaaagcc
     2161 tttgataccg tcttttcgat gggcgtgctt taccatcgcc gttcgccgct ggagcatctc
     2221 tggcagttga aagatcaact ggtgaatgag ggtgaactgg tgctggaaac gctggttatt
     2281 gatggcgatg agaacacggt gctggtacca ggcgatcgtt acgcgcaaat gcgtaatgtc
     2341 tatttcatcc cttccgcgct ggcgctgaaa aactggctga agaagtgtgg ttttgtagat
     2401 atccgcattg tggatgtgtg cgttaccact actgaagagc agcgacgcac tgaatggatg
     2461 gttaccgaat ctctgtccga tttcctcgac ccacatgatc cgagtaagac agttgaaggt
     2521 tatcctgcgc ctaaacgcgc ggtgctgatt gcgcgcaagc cgtaaaggtc tggtattact
     2581 gccggatgcg gcgtgaacgc cttatccggc ctacaaaccc ttgctaattc aatctattgc
     2641 agggactatg taggcctgat aagcatagcg catcaggcag ttttacgttt gcataacctc
     2701 agcgcccgtt tccgggcgct attcacttct tacgcctgaa cagcaggctt atcaaatgcc
     2761 gttaacttcg gtgcgttacc cgtatatttt tcgatataca ccagcgcaga gttaccggca
     2821 caggcatttg cccgctgcga gctggggata tccgccgtta acacgttcgc actgccgttt
     2881 ttacacaagc cgttttccag atctggccat gcaccttcat gcacgcatac cacgcctttt
     2941 ttgatcccgt cagtcaccac cgcgcctgtc aaaatctgac cacgtttgtt ccagacacgc
     3001 accagatcgc cattcgcaat accaaatcga gcagcatctt cggtgtgaat agtgatcggt
     3061 tcacgatcgg caaccgcata ttttttacgc agttccgcat agttaagctg actgtgtaaa
     3121 cggtgtgccg gatgtgcggt cagaagctgc aactgcttct cgtcggcggt acccttccac
     3181 tcatcaggcg caagccaggt tgggtgtgcc gggcaatcct tatagccaaa tttttccagc
     3241 gttttggagt aaatctcaat tttgccgctt ggcgtaccca gcgcattttt caccggatcg
     3301 gcgcggaaat caccataacg aacgtactgt tcgttcttct cgctgcggcg catttcgatc
     3361 agtttatttt gctgccagaa ggcattaaac attggcatag tgacgcgttg cgcacgggcc
     3421 cctttctgag cggcatcata gaaaaatttc agccacgcca tttcatcttt accttcggta
     3481 tagatctctt ttccgccagg tttgagtaat tccgcaagat cggcaaacac gtcaaaatcg
     3541 ttacgcgctt caaattgcgg agcaacagcc tgcttcatcg gcacaatatg ctggttgctg
     3601 taatcaccgg tcatcgtcaa gtcattgcgc tcaaacgatg tggtgatcgg taatacgata
     3661 tctgcgtgtt tggctgctgc ggtccagtag cattcagaaa cgacgatcat ctccggtttc
     3721 tgccaggctt tgatcagacg attagtatcc tggtgatggg taaagttacc gccacccgcc
     3781 caccagatca ttttgatatt cggatacgtt tgttctttac cgttatgctg atatttgccg
     3841 ccaggatttt ccagcgcatc gacgatacgg gcaacaggaa tcgccgtcat tccgccatca
     3901 tcagcggcct cgctggcctg tccggcaatc gccgcagaca tttccggcag cacgccgcca
     3961 acacgcgtcg ggttgccacc attggagtaa tgataagaga aaccaaaacc gccgcccggt
     4021 gtaccaatct gccctaacat cgccgccagc gtcaccagca tccagtgttt ctgttcacca
     4081 tattgctggc gctgaattcc ccagcctgcc atcagcattg tgcgattcgc cgccatcagt
     4141 tcagcgagtt tgactatctg cgcttccggt acgccagtaa tttccgccgt ccagaccgcg
     4201 ctcttcggcg tattatcgct cttacctgtc agatactctt caaactgcgg atacccggta
     4261 gtgtattttt caaggaacac tttatcgtgt ttgccttgtg tcatcagggt atgcgcaatc
     4321 cctaacatca gtgccacgtc ggtgcccata ttcggcgcga tccaggtggc attatcgcca
     4381 aagaattcga tggtttcgga gcggatagga tcaatggcaa tcactggttt gccagatttt
     4441 ttcagctgat ggaagtattc cagcccttgc tcatcggtac tgctccaggc aatttttaag
     4501 gtattcagcg ggttcattcc ccacagcacc acaacctggc tgttttccag aatcagcggc
     4561 caggaggcct gctgttcata cacctctaca gaaccgacca catgcggcat gatcacctgt
     4621 gctgccccgg ttgaataatc gccgctatgc ccggaataac cgcccgccag gttcatataa
     4681 cgttgcagta aggtttgcgc tttatgcaac acgccagaag agcgccagcc gtaagatccg
     4741 gcaaaaatgg ccgatggtcc gttagcttta cgaatacgct catgttgctc atgaatcagt
     4801 tttaatgcct gttcccagct cacctgtaca taggtatctt cgccacgacc tttcgccggt
     4861 tgcagtggat tgtcgagata gctttttctc accatcggat gctgaatacg cgccgtggtg
     4921 tgtacctgat ccgccgccgt agactgtaag gagttcggta tggttttcgc cagcgcgcct
     4981 gtcgaagaaa caatcttgcc gtccttcact tctacgctca tcgctcccca acgtcccgcg
     5041 gtgaggattt taccgccctt ctcttctgcc catgcgggga gcggtgctgc cgatgtcacc
     5101 accagcgctc cagcggcaat accgctgtgt ttaataaatt cacgtcttgt taatgccata
     5161 acttcctccc tgatcaacga ggatcactgt ttctcggtaa tatctttggc gttgtactgg
     5221 aaataccgcg ttaaaatgtc cggttcgttt tcgctcatgc tggttcgtgc ccccattcct
     5281 ttggcaatgg acggccacgc attgacggtg taatggtcgg cggcaataga ggcatgacaa
     5341 ccagcgcaat aggtatcgtc aagtttttca gcgtattgcc atagcggttt acggtccgct
     5401 aatgcgggat cggtaagcgc accctgtaaa gacgcctgac gccattgatt gccgtattcg
     5461 tcagcctgcc attccccgtt taccgtgagc gccttgatac cttcttcact taatgtggct
     5521 agcgccagcc gttgacctgc cgccaggtag agcgtgtttt cactgccctg catttgataa
     5581 ccctgcaaca gaacgatcgg ctgtttgcca ctggcatcaa cgacggtgag atcggtacca
     5641 ggattcacgg tagccagctc gcctatgcga gaagttttga aaggataaat atgtgcgcca
     5701 ttagtcactg aagtagcggc ctgactttcc agctcatgcg ccgcgttgtc atccattttt
     5761 atttctggcg gaaaatgggc aatgccttta tgacaatcga tacaggtttc gccgcccttt
     5821 tgtgctttgt tatgcatttt ctgcgcagat tcactttgcg aggcaatatc catggcatca
     5881 aaagaatggc aactacggca cgttgcagag tcagttgctt ttaattcttt ccatactgtt
     5941 tcggccattt cctggcgatg agtttcgaac ttatcgtcac tgtctatttt gccgctaaca
     6001 aattcatgat aaatatcttt agatgctttt aatttagcaa ataaataatc catccctgac
     6061 tttggaatat ggcaatcggc acattccgca cgtatccctt tctggttcga aaagtggaca
     6121 gttccctgat attcctcaaa aggtttactc atcgagtggc aggaaaggca aaatgctgta
     6181 tccgacgttt tatgtaaggc tttttgcgcc agcaataacc cgccgccacc aaccacaacc
     6241 gctatcagca aaaacaataa cccaatgcgt tttttccctc gcatagattc ctctaaataa
     6301 attccagaac accgaaatca ttgtataatt aaatacacaa cgaattaacc gcttgcataa
     6361 ctcggcacaa cactgcctga aataatcgat aaagaatatg atttattaca atgtaatcat
     6421 taattgctaa ggaataaccc agttgctatt tagaatatat atttatcagt tttagcaatt
     6481 taaatcccat aatcaatgtg aatataaaaa atatttttag cacaacgcta tgcgcactgt
     6541 cacccgctca acagatcacg gttatcgttc gttttttata ctgctcaggg ataaaaaaag
     6601 gcccctgttg aaattgcagg ggcctggtac gagcaagcat catattgggc gacatgatgc
     6661 aacggtaaaa atcatttggc ctgatggcgt tcaatgattc ctttcatttc agcaaccgcc
     6721 gccccgtcta cgatataacg cgaatactcg tccgcgtgtt catctgatga catggacaat
     6781 ccttgattac gataacgcat cggtgaggct tgccacgctc ccgcggagct atggacttcc
     6841 agcactccgg catcgaggaa gtggtgcagg ttttctgcac ggactcctgc tccagccata
     6901 atgattggag catcacgatg ggcaataagt tccataattt ttgataaacc ttgcagtgcg
     6961 tctgattttt gccctgatgt cagtacccgg gcaatgccca attccgcaag attattgagt
     7021 gtatataaag ggttagcgca catatcgaag gcgcgatgaa aagtcactgc cagcggaccg
     7081 gcagcagcca ttattttttc cattcgtggc atatcgacat tcccgtcaac atcgagaacg
     7141 cccgtcacca gtccaggaaa acctaattcg cggaccgtgc gcacatcctc aagaatggcg
     7201 gcaaactcac cgtcgctgta gcaaaaatca ccaccgcgtg ggcgaattat cggatgcaca
     7261 gggatcgtca cccgctggcg cacggatttc agtaccccca acgacggcgt taagcccccc
     7321 tcttttgggg ctgcgcataa ttcaactctg tctgcgccgt tttgctgcgc cgttagtgca
     7381 cattccatgc tgtaacagca aatttccagt aatgccattt ttgctcctta attacgctga
     7441 ctgctcgctg gcaacgatct cttcaatgga ccacggatga aacttaatag ttgttttgcc
     7501 atcggtgacc gccagcgtcg gattcggtaa tcgctcatgc tcaccttttg gtgaactggt
     7561 tttaactgag atccccggta ggctgagtcc ttcatcagaa agcaatgcca gcgcacgggc
     7621 attcagcgtt tctggatcac ccggcagaac gatttcaata tgttcccaac cttcgtgtgg
     7681 gtaacgtttt tccccgggcc acggtagctc cacattggaa aactgccagt gcgcaacctg
     7741 taccggttca tgcaatttaa acagacaaat cggtctgcca ttgatcattt tttctgacaa
     7801 aagctcgcca cactgttcaa aaccgcgacg ccagcgctca gcggtgacgt tttgatggca
     7861 acgcaaagaa atgtgatcgg cagtcagcgg agtgatatcc aaccccagac ggcgggaaag
     7921 ttcatctaat gcgtgggtaa atcgcggtaa atccgatgca atatcctgca gctcgtcgat
     7981 agattgccag ttcgccataa tcactcttcg tctttcagta aaagcgttaa tttaccctgt
     8041 tgccctgtgc caaccaaccg ctgatttcac gccgcttctg atgcaatagt gaaaacggca
     8101 atacgccacg cgcacgttgc tgacgaaaac agccatttgc agtatactcc cgccctaatt
     8161 tctttaactg gtgcgggcaa tttttgctcg cttcatcaat gtaaggtatt ccggtgaata
     8221 ttcaggctct tctctcagaa aaagtccgtc aggccatgat tgcggcaggc gcgcctgcgg
     8281 attgcgaacc gcagattcgt cagtcagcaa aagttcagtt cggcgactat caggctaacg
     8341 gcatgatggc agttgctaaa aaactgggta tggcaccgcg acaattagca gagcaggtgc
     8401 tgactcatct ggatcttaac ggtatcgcca gcaaagttga gatcgccggt ccaggcttta
     8461 tcaacatttt ccttgatccg gcattcctgg ctgaacatgt tcagcaggcg ctggcgtccg
     8521 atcgtctcgg tgttgctacg ccagaaaaac agaccattgt ggttgactac tctgcgccaa
     8581 acgtggcgaa agagatgcac gtcggtcacc tgcgctctac cattattggt gacacagcag
     8641 tgcgtactct ggagttcctc ggtcacaaag tgattcgcgc aaaccacgtc ggcgactggg
     8701 gcactcagtt cggtatgctg attgcatggc tggaaaagca gcagcaggaa aacgccggtg
     8761 aaatggagct ggctgacctt gaaggtttct accgcgatgc gaaaaagcat tacgacgaag
     8821 atgaagagtt tgccgagcgt gcacgtaact acgtggtaaa actgcaaagc ggtgacgaat
     8881 atttccgcga gatgtggcgc aaactggtcg acatcaccat gacgcagaac cagatcactt
     8941 acgatcgtct caacgtgacg ctgacccgtg atgacgtgat gggcgaaagc ctctacaacc
     9001 caatgctgcc aggaattgtg gcggatctca aagccaaagg tctggcagta gaaagcgaag
     9061 gggcgaccgt cgtattcctt gatgtgttta aaaacaagga aggcgaaccg atgggcgtga
     9121 tcattcagaa gaaagatggc ggctatctct acaccaccac tgatatcgcc tgtgcgaaat
     9181 atcgttatga aacactgcat gccgatcgcg tgctgtatta catcgactcc cgtcagcatc
     9241 aacacctgat gcaggcatgg gcgatcgtcc gtaaagcagg ctatgtaccg gaatccgtac
     9301 cgctggaaca ccacatgttc ggcatgatgc tgggtaaaga cggcaaaccg ttcaaaaccc
     9361 gcgcgggtgg taccgtgaaa ctggcagacc tgctggatga agccctggag cgtgcacgtc
     9421 gtctggtggc agaaaagaac ccggatatgc cagctgacga gctggaaaaa ctggctaacg
     9481 cggttggtat tggtgcggtg aaatatgcgg atctctccaa aaaccgcacc acggactaca
     9541 tcttcgactg ggacaacatg ctggcgtttg agggtaatac cgcgccatac atgcagtatg
     9601 cttacacgcg tgtattgtcc gtgttccgta aagcagaaat taacgaagag caactggctg
     9661 cagctccggt aatcatccgt gaagatcgtg aagcgcaact ggcagctcgc ctgctgcagt
     9721 ttgaagaaac cctcaccgtg gttgcccgtg aaggcacgcc gcatgtaatg tgtgcttacc
     9781 tgtacgatct ggccggtctg ttctctggct tctacgagca ctgcccgatc ctcagcgcag
     9841 aaaacgaaga agtgcgtaac agccgtctga aactggcaca actgacagcg aagacgctga
     9901 agctgggtct ggatacgctg ggtattgaga ctgtagagcg tatgtaatcg atttttcgtg
     9961 agagtgaagc ctgatcaggg ttagccgatc aggctttttt attgccatct aaatgtattc
    10021 tgaaaacgga catgccattg ttttctcact gttgagtaag aggccagaag cgtaatatcc
    10081 aggcccagga aaacgataac ggttcaattt aaggaatacc gaagtgttta aatttcttgt
    10141 attaacatta ggcattatct cttgccaggc ttacgcagaa gatacggtta tagtaaacga
    10201 ccatgacact tcagccatca aagattgttg gcaaaaaaat tcagatgatg atactgacat
    10261 taacgtgatc aaatcatgcc tgcgacaaga atacaatctc gtcgatgcgc aattaaataa
    10321 agcctatggt gaagcttatc gttatataga acaagtgcca cgcacaggtg caaaaaaact
    10381 tgatactgaa caacttaact tgcttaaaaa atcacagcga gcctggctgg attttaggga
    10441 caaagaatgt gaattaatcc tttcaaatga ggatgttcag gatttaagtg acccctattc
    10501 tgaatcagaa tggctatcat gtatgatcat acagaccaat acgcgaactc gccagttgca
    10561 gctataccgt aactctgaag atttttatcc aagccctttg acaagaggat aattcacatc
    10621 tttttggcat gttttgttgc aagctattcc tgataaatga ttgcaacaaa acatccagcc
    10681 ttttgactga gttattaaac atactcgcga gcgcgtaatt ttttgtcttt cagcgataat
    10741 tcacaatcac ttcattccgt tgtaccttta gcggtgg
//
LOCUS       DAJTQQ010000119        10611 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000119 DAJTQQ010000000
VERSION     DAJTQQ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.119"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..1009)
                     /gene="malI"
                     /locus_tag="OO309_003482"
     CDS             complement(<1..1009)
                     /gene="malI"
                     /locus_tag="OO309_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR5848493.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PA"
     gene            1184..2776
                     /gene="malX"
                     /locus_tag="OO309_003483"
     CDS             1184..2776
                     /gene="malX"
                     /locus_tag="OO309_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR5848494.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2786..3958
                     /gene="malY"
                     /locus_tag="OO309_003484"
     CDS             2786..3958
                     /gene="malY"
                     /locus_tag="OO309_003484"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR5848495.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4062..5063
                     /gene="add"
                     /locus_tag="OO309_003485"
     CDS             4062..5063
                     /gene="add"
                     /locus_tag="OO309_003485"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR5848496.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5099..6139)
                     /locus_tag="OO309_003486"
     CDS             complement(5099..6139)
                     /locus_tag="OO309_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5848497.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6382..6507
                     /gene="blr"
                     /locus_tag="OO309_003487"
     CDS             6382..6507
                     /gene="blr"
                     /locus_tag="OO309_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR5848498.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6780..6995
                     /gene="ydgT"
                     /locus_tag="OO309_003488"
     CDS             6780..6995
                     /gene="ydgT"
                     /locus_tag="OO309_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR5848499.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7081..7521
                     /locus_tag="OO309_003489"
     CDS             7081..7521
                     /locus_tag="OO309_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR5848500.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7598..8179
                     /gene="rsxA"
                     /locus_tag="OO309_003490"
     CDS             7598..8179
                     /gene="rsxA"
                     /locus_tag="OO309_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR5848501.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8179..8757
                     /gene="rsxB"
                     /locus_tag="OO309_003491"
     CDS             8179..8757
                     /gene="rsxB"
                     /locus_tag="OO309_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR5848502.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8750..>10611
                     /gene="rsxC"
                     /locus_tag="OO309_003492"
     CDS             8750..>10611
                     /gene="rsxC"
                     /locus_tag="OO309_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR5848503.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKA"
BASE COUNT         2440 a         2751 c         2710 g         2710 t
ORIGIN      
        1 gggcgggaat aataagattg cgtgaatgcg tctcttcatg ggtgattttt tgcatcatgc
       61 gatccgcaag tgtgatacca agttcccgcg ctggcgtgct ggcccaggta acaggtatat
      121 catcaagtgt ggttggtgtc gcatcggtaa atgccgccag cgaaacctgt tgctcaaagt
      181 aacgatcgac tccgctttcc ccgctttgcc gccctgcttt cagcaaacca aaccatgccc
      241 ccatcgcaat agtttcgtta tagcaaacca cggcactgat ggtcgggtta tgacgtaaaa
      301 gcgccgtgat agcttccgcg gcttgcttct ggctggaagt gcactccaac acccaatcgc
      361 tgtgaaacgg caggccaaat tttagtagag ttgcacaata gccccccacc cgttctgcac
      421 gggttaatga ggaactttgc cctcccagcc aggcgatccg ctgatgccca ttgcgaatga
      481 gatgctccgt caacaactgt gcagcctgca tgttgtccgg gcgaaccgta tcaacatcat
      541 caagataact ggcacgggaa gcgaaaatca ccgggatagc tttttcttct gccatccgcc
      601 gcaggtcatc gctgcttcct gcagccccgg caattaccac accatcgaca ccctgattca
      661 gtaacagtga aaaccgctgt gccagctgct cgccgtcttt accgccgtga agcaaaaaaa
      721 ccatccgtcc ctgtgcttcc agagcttccg tcaatccggc cgtcaattcg gcgtaaaacg
      781 gcgcagataa atcacggacg atcaaaccaa tgacgccgct ttgcccgccg cgcagcgccg
      841 acgcctggcg attgcgcaca aatcccagct cttcaatggc ggcgttaacg cgttctcctg
      901 tggcggtaga gattcgccct ttgccattaa gcaccagcga aacggtactt accgacacgc
      961 ccgcagccag cgcaacatca tgaatggtta tttttttggc ggtagccatg cagatgacct
     1021 actccctgat tatgtgacag ataaaacgtt ttacctttta tttatcttat acccgctatt
     1081 atcgttgcgt aatgtgattt atgactcact aaaatttgat aaaacgtttt atcttctcgc
     1141 gcaatttact gaatccagat tgttctctac gaggagtcgt tttatgacgg cgaaaacagc
     1201 accgaaagtc acgctgtggg agttcttcca gcagttaggc aaaaccttca tgttacccgt
     1261 ggcattattg tcgttctgcg gcattatgct cggcattggt agttctctta gcagccatga
     1321 tgtcataacc ctgatcccgg tcctgggcaa ccccgtgttg caggctatct ttacctggat
     1381 gagtaagatt ggctcgtttg cttttagttt cctgcctgtc atgttctgta tcgccatccc
     1441 gctgggcctg gcacgcgaaa ataaaggcgt agcggcattc gctggcttcg tcggttatgc
     1501 ggtaatgaac ctcgcggtaa acttctggtt gaccaataaa ggcattctgc caaccacaga
     1561 tgccgccgtt ctgaaagcca ataacatcca gagcattctt gggatccagt cgatcgatac
     1621 cgggatcctc ggtgcggtga tcgccggtat tatcgtctgg atgctgcatg agcgtttcca
     1681 taatatccgc ctgccggatg cgctggcatt cttcggcggt acgcgcttcg taccaattat
     1741 ctcctcgctg gtgatgggcc ttgtcggcct ggtgattcca ttagtctggc cgattttcgc
     1801 catgggtatt agcggcttgg gccatatgat aaacagcgcg ggtgatttcg gaccgatgct
     1861 gtttggtacc ggtgaacgtc tgctgttgcc gtttggtctg catcacattc tggtggcatt
     1921 aattcgcttt accgacgcag gcggcacgca ggaggtctgc ggtcaaaccg tcagcggcgc
     1981 actgaccatc ttccaggcgc aattgagttg cccgaccact cacggttttt ctgaaagcgt
     2041 cacgcgtttc ctttcgcaag gtaaaatgcc tgcgtttctc ggcggtctac caggtgcagc
     2101 gttagctatg tatcactgcg cgcgcccgga aaatcgccat aaaattaaag gtctgctgat
     2161 ttctggcctg atcgcctgcg tcgttggcgg cactaccgaa ccgctggaat tcctgttcct
     2221 gttcgtagcg ccagttctgt atgtcatcca cgcgctgtta accggcctcg gcttcaccgt
     2281 catgtctgtg ctcggcgtca ccatcggtaa taccgacggc aatatcatcg acttcgtggt
     2341 gttcggtatt ttgcatggtc tgtcaaccaa gtggtacatg gtgccagtgg tggcggcaat
     2401 ctggtttgtc gtttactacg tcatcttccg tttcgctatc acccgcttca atctgaaaac
     2461 cccggggcgc gatagcgaag ttgccagctc aatcgaaaaa gccgttgccg gtgcgccggg
     2521 taaatcaggt tacaacgttc ctgcaatcct cgaagcatta ggcggtgccg acaatattgt
     2581 cagcctcgat aactgcatta cccgtctgcg tttgtctgtg gaagatatgt cgcttgttaa
     2641 tgtgcaggca ctgaaggaca atcgggcaat tggcgtagta caacttaatc aacataacct
     2701 gcaggttgtt atcgggccac aagttcagtc agtaaaagat gaaatggccg gtctgatgca
     2761 tactgtccag gcataaggat aagatatgtt cgatttttca aaggtcgtgg atcgtcatgg
     2821 cacatggtgt acacagtggg attatgtcgc tgaccgtttc ggcactgctg acctgttacc
     2881 gttcacgatt tcagacatgg attttgccac tgccccctgc attatcgagg cgctgaatca
     2941 gcgcctgatg cacggcgtat ttggctacag ccgctggaaa aacgatgagt ttctcgcggc
     3001 tattgcccac tggttttcca cccagcatta caccgcaatt gatacgcaga cggtggtgta
     3061 tggcccttct gtcatctata tggtttcaga actgattcgt cagtggtctg aaacaggtga
     3121 aggcgtggtg atccacacgc ccgcctatga cgcattttac aaggccattg aaggtaacca
     3181 gcgcacagta atgcccgttg ctttagagaa gcaggctgac ggctggtttt gcgatatggg
     3241 caagctggaa gccgttttgg cgaaaccaga atgtaaaatt atgctcctgt gtaacccaca
     3301 gaatcctacc gggaaagtgt ggacgtgcga tgagctggag atcatggctg acctgtgcga
     3361 gcgtcatgat gtgcgggtta tttccgatga aatccatatg gatatggtct ggggcgagca
     3421 gccgcatatt ccctggagta atgtggcgcg gggagactgg gcgttgctga catcgggatc
     3481 gaaaagtttc aatattcccg cgctgaccgg tgcttacggg attatagaaa atagcagcag
     3541 ccgtgatgcc tatttatcgg cactgaaagg acgtgatggg ctttcttccc cttcggtact
     3601 ggcgttaact gcccatatcg ccgcctatca gcaaggtgca ccgtggctgg atgccttacg
     3661 cgtctatctg aaagataacc tgacgtatat ctcagataaa atgaacgccg cgtttcctga
     3721 actcaactgg cagatcccgc aatccactta tctggcctgg cttgatctac gtccgttgaa
     3781 tattgacgac aacgcgttgc aaaaagcgct tatcgagcaa gaaaaagtcg cgatcatgcc
     3841 ggggtatacc tatggtgaag aaggtcgtgg ttttgtccgt ctcaatgccg gctgcccacg
     3901 ttcgaaactg gaaaaaggtg tggctggatt aataaacgcc atccgcgctg ttcgctaacc
     3961 ccaattgcgc aacgtaaaaa atcgttgcgc aatcgtggat ttttaccctg ctttgttttt
     4021 ataatggtgc gcacttttat atccagaaaa agagtgcgac catgattgat accaccctgc
     4081 cattaactga tatccatcgc caccttgatg gcaacattcg tccccagacc attcttgaac
     4141 ttggccgcca gtataatatc tcgcttcctg cacaatccct tgaaacactg attccccacg
     4201 ttcaggtcat tgccaacgaa cccgatctgg tgagctttct gaccaaactt gactggggcg
     4261 ttaaagttct cgcctctctt gatgcctgcc gccgcgtggc atttgaaaac attgaagatg
     4321 cagcccgtaa cggcctgcgc tatgtcgagc tgcgtttttc accaggctac atggcaatgg
     4381 cacataagct gcctgtagcg ggtgttgtcg aagcggtgat cgatggcgta cgtgaaggtt
     4441 gccgcacctt tggtgtgcag gcgaagctta tcggcattat gagccgaacc ttcggcgaag
     4501 ccgcctgtca gcaagagctg gaggcctttt tagcccaccg tgaccagatt accgcacttg
     4561 atttagctgg tgatgaactt ggattcccgg gaagtctgtt cctttctcac ttcaaccgcg
     4621 cgcgtgatgc gggctggcat attaccgtcc atgcaggcga agctgccggg ccggaaagca
     4681 tctggcaggc gattcgtgaa ctgggtgcgg agcgtattgg acatggcgta aaagccattg
     4741 aagatcgggc gctgatggat tttctcgccg agcaacaaat tggtattgaa tcctgtctga
     4801 cctccaatat tcagaccagc accgtagcag agctggctgc acatccgctg aaaacgttcc
     4861 ttgagcatgg cattcgtgcc agcattaaca ctgacgatcc cggcgtacag ggagtggata
     4921 tcattcacga atataccgtt gccgcgccag ctgctgggtt atcccgtgag caaatccgcc
     4981 aggcacagat taatggtctg gaaatggctt tcctcagcgc agaggaaaaa cgcgcactgc
     5041 gagaaaaagt cgccgcgaag taacaaaaat ggatggtgca aattgcacca tccatttttc
     5101 acgcaaggca caacgtcgcg cgatgtttgg cggattcgat gcccagctca atcaactcca
     5161 ttacctggat tgcctggctt gccggaaccg gattttcacc atcgccattt aacgcatcac
     5221 gaatcgccgc atagtaagcc ggataattcc caggcaccgt caacagcgtt tcttcgacac
     5281 gttcctcacc ttccacgcgg gtaagtacgc catcacgcat atcgtagccc cagtcttcct
     5341 gcggcagacg ctcgccattt ttcagacgtt cttcctgtgg atcgaggcca tatttcacat
     5401 aactgcctcg ggatccatgc acgatatacc gtgctgactc agcggctgcc agcatggtac
     5461 cgtgtaaaat gactcgccgc tgtggatagg acaagatggc gtggaaataa tcggtcgact
     5521 gcgctccggg ccgtaactgt gccaaatcta ccgtcatgct gaccggtaaa ccaaatagcg
     5581 taatggcctg atcaagaaga tgcggtgcta aatcgtacca gataccgctg cctgggccgc
     5641 cctgttcacg ccaacgatcg cgcacctgcg gacggaagcg gtcaaaatga gactcaaagt
     5701 aagcaacttc acccagcacg ccttccgcga gcaaaccttt tagcgtcaag aaatcgctat
     5761 cccaacggcg gttatggaat acagacagca cacgccccag gctttttgcc agcgcatcca
     5821 gctctcgcgc ttgtgacagt gtcacggtaa agggtttatc aacgaccaca tgtttacccg
     5881 cctcaagcgc cgctttggct aacgggaaat gggtatcgtt gggtgtagga atgacaatca
     5941 ggtctatgtt gggatcgtta aacagatgct tcggctcaga gacaaccgta accgttggcc
     6001 agtcggcttt tacttttgtt tcatcactgc tggagattac cgccagttcc agcccgggcg
     6061 tgcccgcaat caggggcgca tggaaggttt tgctcgcata accataccca atcaacccaa
     6121 cacggatgtt gtcgctcatg tcattgcctc tcattgaagt atgatggcta tttgacacta
     6181 tcctttaccc acgctcaaca gtttaataac ctgccagcaa taagggatgt tgtttaactt
     6241 aagtcaaaaa aatagcgaat tttccaacga caaaagctaa atatcgcaaa aacctcagta
     6301 aaaatcttgc tggagctatt attgctaagt aacatttacc ccctgaagtt aatggatcaa
     6361 tcaagagaga tgtgggctgt aatgaatcgt cttattgaat taacaggttg gatcgttctt
     6421 gtcgtttcag tcattcttct tggcgtggcg agtcacattg acaactatca gccacctgaa
     6481 cagagtgctt cggtacaaca caagtaagct ctgcacttgt ggagcgacat gctgcccgtc
     6541 cgggtgcatg ttttcacttg tcggatatta aaccaggaat ttattatctt gttcgatgtt
     6601 gttggtgatt gtcagggata gtaaagcgcg ttactctttt cagagcagtt tttcatccct
     6661 gaatatttgc catcagcgca ttcttattat tgtaataaca caagcgttca cttttggtga
     6721 aattttatca tcactaataa tacctacccc tttggtatta attattttat ggacctttta
     6781 tgactgttca ggactactta ttaaaatttc gcaaaatcag ttcactcgaa agtctggaaa
     6841 aactctacga ccatcttaat tacaccctga cggacgatca ggaactgatc aatatgtatc
     6901 gtgctgccga tcaccgtcgc gcagagctgg tttctggcgg gcgtttgttt gacctcggcc
     6961 aggtaccgaa gtccgtctgg cactatgtcc aataaagaaa gtagcgatcg gttataaaag
     7021 ccttataata acgcccctaa aattagattt gcctgtttgc gtcgctattc tggagaacct
     7081 atgaccacca caacgccaca acgtattgga ggctggttgc ttggcccgtt ggcctggctt
     7141 ttagtcgctc tgttgagtac gacgctggcg ttgttgctgt ataccgctgc gttatcttct
     7201 ccgcaaacat ttcaaacgct cggcggacaa gcactgacga cacaaatctt atggggcgtt
     7261 tcttttatta ccgctatcgc catgtggtat tacacactgt ggttgaccat cgctttcttt
     7321 aaacgtcggc gttgtgttcc taaacactat attatctggc tgcttatttc cgtattgctg
     7381 gcagtaaaag cattcgcttt ttcacccgtg gaagttggca tagccgttcg ccagttgctg
     7441 tttactttgt tggcaaccgc attgatcgtc ccctatttca aacgctcgtc gcgggtaaaa
     7501 gcgacgtttg tgaatccgta ataaccttac agttaacctg ttgtcgcctg ctctggatta
     7561 acggataata ggcggctttt ttatttcagg ccgaaaaatg actgactacc tgttactgtt
     7621 tgtcggaact gtactggtca ataactttgt actggtcaag tttctcggtc tctgtccgtt
     7681 tatgggggtt tccaaaaaac tggaaaccgc gatgggcatg gggctggcaa caacgtttgt
     7741 gatgacgctg acgtctattt gcgcctggct tatcgatacg tggattttga tcccacttaa
     7801 tctgatttac ctgcgcaccc tggcatttat tctggtgatt gctgtggtcg tgcagttcac
     7861 cgagatggtg gtgcgcaaaa ccagcccggt gctttaccgt ctgctgggga tttttttgcc
     7921 gcttatcacc accaactgtg cagtgctcgg cgtggcgttg ctgaatatca atctcgggca
     7981 caatttcttg cagtcggcgc tgtacggttt ttccgccgct gtcggtttct cgctggtgat
     8041 ggtgctcttc gccgccatcc gcgaacgcct tgctgtggct gatgtcccgg caccttttcg
     8101 cggtaatgcc attgcgttaa ttaccgcagg tcttatgtct ctggccttta tgggctttag
     8161 tggtttggtg aagttgtaat gaatgctatc tggattgccg ttgccgccgt gagcctgctg
     8221 ggcctggcgt ttggcgccat tctgggttat gcctcccgcc gttttgcggt ggaagacgat
     8281 ccggtcgttg agaaaattga cgaaatctta ccgcagagcc agtgtggtca gtgcggttat
     8341 cccggctgtc gcccctacgc ggaagccatc agctgtaacg gtgaaaaaat caaccgttgc
     8401 gccccaggtg gcgaagctgt gatgctaaaa attgccgagt tgcttaatgt cgagccgcag
     8461 ccgctggatg gcgaagcgca agagctaacg cctgcgcgga tggtggcggt tattgatgaa
     8521 aataactgta ttggctgcac taaatgtatt caggcttgtc cggtagacgc catcgttggc
     8581 gctacccgtg tcatgcatac ggtaatgagt gatctctgta cgggctgcaa tttatgtgtt
     8641 gatccgtgcc cgacgcactg catctcgttg caaccggtcg cagaaacacc tgactcctgg
     8701 aaatgggatc tgaacaccat tcccgtgcgt atcattcccg tggaacacca tgcttaagtt
     8761 attctctgca ttcagaaaaa ataaaatctg ggatttcaac ggcggcatcc atccaccgga
     8821 gatgaaaacc cagaccaacg gtacccccct gcgccaggta ctactggcgc agcattttgt
     8881 tattccactg aaacagcata ttggcgctga aggtgagttg tgcgttagcg tcggcgataa
     8941 agtattgcgc ggccagccgc ttacccgtgg tcgcggcaaa atgctgcctg ttcacgcgcc
     9001 cacctcgggt accgttacgg ctattgcgcc ccactctacg gctcatcctt cagctttagc
     9061 tgaattaagc gtgattattg atgccgatgg tgaagactgc tggatcccgc gcgacggctg
     9121 ggtcgattat cgctctcgca gtcgcgaaga gttaatcgag cgcatacatc agtttggtgt
     9181 tgccgggctg ggcggtgccg gattcccgac aggcgttaaa ttgcagggtg gtggagataa
     9241 gattgaaacg ttgattatca acgctgctga gtgcgagccg tacattaccg ccgatgaccg
     9301 tttgatgcag gattgcgcgg ctcaggtcgt agagggtatt cgcattcttg cgcatattct
     9361 gcagccacgc gaaattctta tcggcattga agataacaaa ccgcaggcga tttccatgct
     9421 gcgcgcggtg ctggcggact ctaacgatat ttctctgcgg gtgattccaa ccaaatatcc
     9481 ttctggcggt gctaaacaat taacctacat tctgaccggg aagcaggttc cacatggcgg
     9541 gcgttcatcc gatatcggcg tattaatgca aaacgtcggc actgcttatg cagtgaaacg
     9601 tgccgttatt gatggcgagc cgattaccga gcgtgttgta accctgactg gcgaagcaat
     9661 cgctcgcccg ggcaacgtct gggcacggct ggggacgcca gtgcgtcatt tattgaatga
     9721 tgccggattc tgcccctctg ccgatcaaat ggtgattatg ggtggcccgc taatgggctt
     9781 taccttgcca tggctggatg tcccggtcgt aaagattacc aactgtctgt tggctccctc
     9841 tgccaatgaa cttggcgaac cacaggaaga acaaagctgc atccggtgta gcgcctgtgc
     9901 tgacgcctgc cctgtggatc ttttgccgca acagttgtac tggttcagca aaggtcagca
     9961 acacgataaa gctaccacgc ataacattgc cgactgcatt gaatgtgggg cttgcgcgtg
    10021 ggtttgcccg agcaatattc ccttggtgca atatttccgt caggaaaaag ctgaaattgc
    10081 ggctattcgt caggaagaaa agcgcgccgc agaagccaaa gcgcgtttcg aagcgcgcca
    10141 ggctcgtctg gagcgcgaaa aagcgactcg ccttgaacga cataagagcg cagccgttca
    10201 acctgcagcc aaagataaag atgcgattgc tgccgctctg gcgcgggtga aagagaaaca
    10261 ggcccaggct acacagccta ttgtgattaa agcgggcgaa cgcccggata acagtgcaat
    10321 tattgcagaa cgggaagccc gtaaagcgca agccagagcg aaacaggcag aactggagca
    10381 aactaacgac gcagcaaccg ttgctgaacc acgtaaaact gccgttgaag cagctatcgc
    10441 ccgctccaaa gcgcgcaagc tggaacagca acaggctaat gcggaaccag aagaacaggt
    10501 cgatccgcgc aaagccgccg tcgaagccgc tattgcccgt gccaaagcgc gcaagctgga
    10561 acagcaacag gctaatgcgg aaccagaaga acaggtcgat ccgcgcaaag c
//
LOCUS       DAJTQQ010000120        10396 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000120 DAJTQQ010000000
VERSION     DAJTQQ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.120"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(141..215)
                     /locus_tag="OO309_003493"
     tRNA            complement(141..215)
                     /locus_tag="OO309_003493"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(180..182),aa:Arg,seq:cct)
     gene            complement(289..1221)
                     /locus_tag="OO309_003494"
                     /pseudo
     CDS             complement(289..1221)
                     /locus_tag="OO309_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1515..2270
                     /gene="mlaA"
                     /locus_tag="OO309_003495"
     CDS             1515..2270
                     /gene="mlaA"
                     /locus_tag="OO309_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR5848504.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2452..3510)
                     /locus_tag="OO309_003496"
     CDS             complement(2452..3510)
                     /locus_tag="OO309_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848505.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
     gene            complement(3876..5216)
                     /gene="fadL"
                     /locus_tag="OO309_003497"
     CDS             complement(3876..5216)
                     /gene="fadL"
                     /locus_tag="OO309_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR5848506.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            5588..5872
                     /locus_tag="OO309_003498"
     CDS             5588..5872
                     /locus_tag="OO309_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR5848507.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVKSEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6052..7362
                     /gene="fadI"
                     /locus_tag="OO309_003499"
     CDS             6052..7362
                     /gene="fadI"
                     /locus_tag="OO309_003499"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR5848508.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7362..9506
                     /gene="fadJ"
                     /locus_tag="OO309_003500"
     CDS             7362..9506
                     /gene="fadJ"
                     /locus_tag="OO309_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR5848509.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            9709..10194
                     /gene="sixA"
                     /locus_tag="OO309_003501"
     CDS             9709..10194
                     /gene="sixA"
                     /locus_tag="OO309_003501"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR5848510.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2592 a         2398 c         2609 g         2797 t
ORIGIN      
        1 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
       61 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      121 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      181 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      241 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      301 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      361 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      421 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      481 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
      541 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
      601 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
      661 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
      721 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
      781 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
      841 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
      901 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
      961 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1021 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1081 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1141 ttctattttt ttgccgcgct ctttttcttc gctctaaact tcaatttcgt cgctgtgttg
     1201 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1261 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1321 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1381 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1441 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1501 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     1561 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     1621 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     1681 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     1741 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     1801 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     1861 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     1921 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     1981 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2041 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2101 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2161 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2221 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2281 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2341 aggagataaa ccgacaatga cccggacgta atgatgtaaa aatactgatg tttgtcactg
     2401 actatttccg atattcaggc gttgaaaaat caaaaaactg gaaaaataat tttaaatatc
     2461 atcaaaatta acacctagtg cagatagttg attaatttca ttttgatatt tcgtctggcg
     2521 attttgaagt cttttttcaa ctttatcttc gagattctta aagtctttgt atttattttc
     2581 aggatggtca acgtcaactt tgttagcgaa taaagtagaa attatttctt ttacattatc
     2641 gcgtctaaat tttgcatcgt ccacttgaaa attatatctt attgctcgac ctaaacgttt
     2701 aaaaacttcg ttaggttctc tcagtctttc cccgttgagc tcgtttacaa cctgatgatc
     2761 ggaaccagtt gcaaatgatt ctgccatgat agctttactg ctttcaaacc acgctgaagc
     2821 actcctggcg ttttcactac aatattgggt gagactatca ccattaatac gaattcctct
     2881 ttgaatgtgg tagtcgtcct gatcgtagta accgagagat aagtaatcat ttccgttttt
     2941 atttttaatt acaatagcga gagagtcgcc ttttttatct ttaccgtacc aggtaaataa
     3001 gtcagttggt tcgcttttcc cgctctcatc aaattccgtt accgtatgtc tgagtttgtg
     3061 tgatatctta tcgaagataa aaccaggatc tccatcatat atttctttta atgcgctgaa
     3121 gatcatttcc ttgttattga tatcatgagt cttctcaact atgttgtcat taatgatagt
     3181 acggacgagt tctttgtctt tatagttcgc aggtaattct attggttctt ggttgtcgat
     3241 accaatagta atatggtctg tatcattgga gccaatatca atataagtaa ctgaggaatt
     3301 attaatatta gatctataag gatcgtcgtt tactcgtatg gcgtttttta taagctcaag
     3361 tagatgttta atacaggtgc ttattccttc cttaccatta aaaaggtttt tgaactcttg
     3421 tactaatcca ttaatttcat cattgttatt ttcattgatg gattctgggt aagctgcgct
     3481 ggtattgttg attgaaatag atggtagcat gattttattc catgttgatt gatttgttac
     3541 aaatatatat tacctaatga aatagactat caaaaaacgc tcatttattt agaacctatc
     3601 tttaatttga gtgtataagc gcacaatagc caggggggtt gagtatcctg gaaatgcaga
     3661 aaagtaaact ctttgagaag gacgagcaga acgcttgaaa atatgcattt cccttcaggt
     3721 ttgtctgtgg ataccactta ctgatttgaa agggcaagct gccatcaggc tgtttgcaaa
     3781 actcaggagg caagcaatga ggaaactact ttcggtgaag tgggggagtt aaagtcacct
     3841 gcatagcagg tgactttatc caggcgaacg cgttatcaga acgcgtagtt aaagttagta
     3901 ccgaacagcc aggctttacc ttcagactcg aactggtatg ggccttcgtt aattttcacg
     3961 ctctgaccgt gcatataaga aacaccaacg tcgactgaag catctttatt aaatgcgtaa
     4021 gtcgtacctg cactcagcca gaaacggtcc tggtccggaa tggagataga acgattctga
     4081 gccggaactg ggctgtcatc aaaggcgata ccggtacgga aggtccagtt atcatcgtag
     4141 taataagtgg tacccaacgc gatgcggtaa gcatctttaa agccttcatg tttctggaac
     4201 agcgtgtcgc cactggttga ggtcgctttc agctgctgga actgactcca gctggtgtaa
     4261 gccaggctat agtgaatcgc ccactgtgga tcaacacggt tataacctga cacttcccac
     4321 atttcaggca ggttcagcgt cagataaccc gattgcgttg cgccacctgt cgcggtagga
     4381 attggcaaac cgtagttatt aaacgcacga ttaagatcgc tgctgtagtt acctttgaag
     4441 tcaattttca cttcagaacg gtaggtcagt gcatagcggt tatttttatc cagttcatac
     4501 aggattccgg cgttccagcc aaagccccac tggttaccgt tcagatgagc gattttggta
     4561 ttactgtcga taccgttggc ggtagctgcc aatgcttgcc cttgaggagt ttgcccggca
     4621 ggagattgca taatctgacc agcaaccaac tgccccagat cgcctgcgaa acgttcaatt
     4681 ttcgcgcgag cgtagatggc gttgaaacca agaccaaagc tccatgcatt atttaagcga
     4741 tacgcaccgc ttaagttcag gttcatggtt tcaaggtcag ttgtaccccc gacagagccg
     4801 cctgcataag tatcattaaa ctctgttgcc agaccatagt tagaggtaat agaagcgccc
     4861 caaccaaatt ggtcgttaat cggtgcaaca aagtgcatgt tcggaaccca tgccgtaggc
     4921 gcgatgttat cggctttcag gctacgacca gacggagacg ttccgctgat attcacatcc
     4981 gggtcaatat aaaccgcacc cgcagaaaat gtcgggcggt caaacatggt aatcaatgcg
     5041 gggttacggc taacgttacc tgcatcatcg gcaattgcgc cttcccctga ataagcccgg
     5101 cccaggccag aggaagaaaa ttcgtttaac tgaaagcctg ccgaccaggc ctgggtggag
     5161 ataagtgcca ctgcgactgc gagagcagac tttgtaaaca gggttttctg gctcatgacc
     5221 ataacctcaa tgatttattt ttatacaaac tatgttacgt gctgtaacag gagcgcgaag
     5281 tgtagggtct gaggtacatc ttagaaatca gaccagtggc gagagtatag gtcggaccag
     5341 ctagaatgtt gcaagtatgt ttccatcttt tttcaaatat gatctaagaa acgcgatcca
     5401 gctggcaaaa tttggaggtt gcgcactcgc ggattcgggt gaattttgtt ttagatcatt
     5461 tttaagtgtg atttcggtca cttatccgat tttgcagaat taacaactgg agcagcactt
     5521 tccggaagcg taaaatatcc acaacgttta tctggcacct tgggtgcaca tacagaggaa
     5581 atcaacgatg agtaaatgta gtgctgatga aaccccggtt tgctgctgta tggatgttgg
     5641 caccattatg gacaactccg actgcaccgc gtcttacagc cgcgtgtttg caaaccgcgc
     5701 agaagcagaa caaacgctgg cggcgctgac tgaaaaatcc cgtagcgtga aatccgaacc
     5761 gtgcaaaatc tccccgactt tcactgaaga gtctgacggt gtgcgcctgg atatcgattt
     5821 cacttttgcc tgcgaagctg aaatgctgat cttccagctg ggtctgcgtt aattcctctc
     5881 gggtacgtac gcccctgtca gttgctggca ggggcgtttt cttttctttc ttctgtcttc
     5941 actgtgtcat agctcccact ttcctttttc tccgcttggc taaatgtaaa aaaatggtta
     6001 agactatgat caggtcagac cactttattt atttttttac aggggagtgt tatgggtcag
     6061 gttttaccac tggttacccg ccagggcgat cgtatcgcca ttgttagcgg tttacgtacg
     6121 ccttttgccc gccaggcgac ggcttttcat ggcattccca cggttgattt agggaagatg
     6181 gtggtaggcg aactgctggc acgcagcgag atccccgctg aagtgattga acagctggtc
     6241 tttggtcagg tcgtacaaat gcctgaagcc cccaacattg cgcgtgaaat tgttctcggt
     6301 accggaatga atgtgcatac cgatgcttac agcgtcagcc gcgcttgcgc taccagtttc
     6361 caagcggttg caaacgtcgc agaaagcctg atggcgggaa ctatccgagc ggggattgcc
     6421 ggtggggcag attcctcttc cgtattgcca attggcgtca gtaaaaaact ggcgtacgtg
     6481 ctggttgatg tcaacaaagc tcgaaccatg agccagcgcc tgaaactctt ctctcgcctg
     6541 cgtttgcgcg acttaatgcc cgtaccgcct gcggtagcag aatattctac tggcttgcgg
     6601 atgggcgaca ccgccgaaca aatggcgaaa acctacggca tcacccgaga acagcaagat
     6661 gcattagcgc accgttcgca tcagcgtgcc gctcaggcat ggtcagacgg aaaactcaaa
     6721 gaagaggtga tgactgcctt tatccctcct tataaacaac cgcttgtcga agacaacaat
     6781 attcgcggta attcctcgct tgccgattac gcaaagctgc gcccggcgtt tgatcgcaaa
     6841 cacggaacgg taacggcggc aaacagtacg ccgctgaccg atggcgcggc agcggtgatc
     6901 ctgatgactg aatcccgggc gaaagaatta gggctggtgc cgctggggta tctgcgcagc
     6961 tacgcattta ctgcgattga tgtctggcag gacatgttgc tcggtccagc ctggtcaaca
     7021 ccgctggcgc tggaacgtgc cggtttgacg atgagcgaac tgacattgat cgatatgcac
     7081 gaagcctttg cagctcagac actggcgaat attcagttgc tgggtagtga acgttttgct
     7141 cgtgaagtac tggggcgtgc acatgccact ggcgaagtgg acgatagcaa atttaacgtg
     7201 cttggcggtt cgattgctta cggacatccc ttcgcggcga ccggcgcgcg gatgattacc
     7261 cagacattgc atgaacttcg ccgtcgcggc ggtggatttg gtttagttac cgcctgtgct
     7321 gccggtgggc ttggcgcggc aatggttctg gaggcggaat aatggaaatg gcatcagcgt
     7381 ttactcttaa tgttcgtctg gacaacattg ctatcatcac catcgacgta ccggatgaga
     7441 aaatgaatac cctgaaggcg gaatttgcct cgcaggtgcg cgccattatt aagcaactcc
     7501 gtgaaaacaa agagttgcgc ggcgtggtgt ttatctccgc gaaaccggac aattttatcg
     7561 ccggagcaga catcaacatg attggcaact gcaaaacggc gcaagaagcg gaagcactgg
     7621 cgcggcaggg ccaacagttg atggcggaga ttcatgcttt gcccattccg gttatcgcgg
     7681 ctattcatgg cgcttgcctg ggcggtgggc tggagctggc gctggcgtgc cacggtcgcg
     7741 tttgtactga cgatcctaaa acggtgctcg gtttgcctga agtacaactt ggattgttac
     7801 ccggttcagg cggcacccag cgtttaccgc gtctgatagg cgtcagtaca gcattagaga
     7861 tgattctcac cggaaaacaa cttcgggcga aacaggcatt aaagctgggg ctggtggatg
     7921 acgttgttcc gcactccatt ttgctggaag ccgctgttga gctggcgaag aaggatcgcc
     7981 catcttcccg ccctctacct gtacgcgagc gtattctggc ggggccgtta ggtcgtgcgc
     8041 tgctgttcaa aatggtcggc aagaaaacag aacacaaaac tcaaggcaat tatccggcga
     8101 cagaacgcat cctggaggtt gttgaaacgg gattagcgca gggcaccagc agcggctatg
     8161 acgctgaagc tcgcgccttt ggcgaactgg cgatgacgcc gcaatcgcaa gctttgcgta
     8221 gtatcttttt tgccagtacg gacgtgaaga aagatcccgg tagtgatgca ccgcctgcgc
     8281 cattaaacag cgtgggaatt ttaggtggtg gcttgatggg cggcggtatt gcttatgtca
     8341 ctgcttgtaa agcggggctt ccggtcagaa ttaaagatat caacccgcag ggcataaatc
     8401 atgcgctgaa gtacagttgg gatcagctgg agggcaaagt tcgccgtcgt catctcaaag
     8461 ccagcgaacg tgacaaacag ctggcattaa tctccggaac gacggactat cgcggctttg
     8521 cccatcgcga tctgattatt gaagcggtgt ttgaaaatct cgaattaaaa caacagatgg
     8581 tggcggaagt ggagcaaaat tgcgccgctc ataccatttt tgcttcgaac acgtcatctt
     8641 taccgattgg tgatatcgcc gctcacgccg cgcgacctga gcaagttatt ggcctgcatt
     8701 tcttcagtcc ggtggaaaaa atgccgctgg tagagattat tcctcatgcg ggtacatcgg
     8761 cgcaaaccat cgccactacg gtaaaactgg cgaaaaaaca gggcaaaacg cccattgtcg
     8821 tgcgtgacaa agccgggttt tacgtcaatc gcatcttagc gccttacatt aatgaagcta
     8881 tccgcatgct gaccgaaggt gaacgggtag agcacattga tgccgcgcta gtgaaatttg
     8941 gttttccggt aggcccaatc caacttttgg atgaggtagg aatcgacacc gggactaaaa
     9001 ttattcctgt actggaagcc gcttatggag aacgttttag cgcgcctgca aatgttgttt
     9061 cttcaatttt gaacgacgat cgcaaaggca gaaaaaatgg tcggggtttc tatctttatg
     9121 gtcagaaagg gcgtaaaagc aaaaaacagg tcgatcctgc catttacccg ctcattggcg
     9181 cacaagggca ggggcgactc tccgcaccgc aggttgctga acggtgtgtg atgttgatgc
     9241 tgaatgaagc agtacgttgt gttgatgagc aggttatccg tagcgtgcgt gacggggata
     9301 ttggtgcggt atttggcatt ggttttccgc catttctcgg tggaccgttc cgctatatcg
     9361 attctctcgg cgcgggcgaa gtggttgcaa taatgcaacg actggccacg cagtatggtt
     9421 cccgttttac cccttgcgag cgtttggtcg agatgggcgc gcgtggggaa agtttttgga
     9481 aaacaactgc aactgacctg caataagaag gtcaaagcta tatgaatccg cgctgaatgg
     9541 cggagtgttg gtcaaaatgt aaacgcatat tgactatact tacgccattg aggtaaaaaa
     9601 cagcgtttca ttcggtgaat ggataaggca caatgccggc caccgttttc tttctctggt
     9661 ttcagatgaa agaaaacggg cgaatctggt taacaaaagc ggtgcaatat gcaagttttt
     9721 atcatgcgtc acggcgacgc agccctcgat tccgccagtg attcggttcg tcctctgacc
     9781 actaatggtt gtgacgaatc tcgcctgatg gcgaactggc tgaaaggtca aaaagtggaa
     9841 atcgaacgcg ttctggtgag cccgttcctg cgagccgagc aaacactgga agaagtaggg
     9901 gattgtctga acctgccttc cagtgcagaa gttctgccgg agttaacgcc ctgcggcgat
     9961 gttggtctgg tcagtgccta tttgcaggcg ttgaccaatg aaggtgttgc ttcggtactg
    10021 gtgatctctc acttaccgtt agtcgggtat ctggttgccg agttatgtcc gggcgaaacg
    10081 ccgcctatgt ttaccacctc ggccattgcc agcgtaacgc ttgatgaaag cggaaacggt
    10141 acattcaact ggcagatgag cccgtgtaat ctgaagatgg caaaagctat ctgatgtgtt
    10201 ccaaattatg cccaatgagg tagattcatt gggcataatt gctcattagg cagcggatat
    10261 ctgtcaacct cactactatc gaagactctc cttatctata aataaaacac tttctacatt
    10321 cattcggaaa ttattcttct acgcgtatct tgattttgcc caaagttaca tgttgaaatg
    10381 aataatatct gtttga
//
LOCUS       DAJTQQ010000121        10327 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000121 DAJTQQ010000000
VERSION     DAJTQQ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.121"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            2..202
                     /gene="cspG"
                     /locus_tag="OO309_003502"
     CDS             2..202
                     /gene="cspG"
                     /locus_tag="OO309_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001990088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCR5848511.1"
                     /translation="MTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNEN
                     QKVEFSIEQGQRGPAAANVVTL"
     gene            213..401
                     /locus_tag="OO309_003503"
     CDS             213..401
                     /locus_tag="OO309_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR5848512.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            376..606
                     /gene="ymcE"
                     /locus_tag="OO309_003504"
     CDS             376..606
                     /gene="ymcE"
                     /locus_tag="OO309_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HCR5848513.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            596..769
                     /locus_tag="OO309_003505"
     CDS             596..769
                     /locus_tag="OO309_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR5848514.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(818..1891)
                     /locus_tag="OO309_003506"
     CDS             complement(818..1891)
                     /locus_tag="OO309_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5848515.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1993..4704)
                     /gene="torS"
                     /locus_tag="OO309_003507"
                     /pseudo
     CDS             complement(1993..4704)
                     /gene="torS"
                     /locus_tag="OO309_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4787..5815
                     /gene="torT"
                     /locus_tag="OO309_003508"
     CDS             4787..5815
                     /gene="torT"
                     /locus_tag="OO309_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR5848516.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5788..6480)
                     /gene="torR"
                     /locus_tag="OO309_003509"
     CDS             complement(5788..6480)
                     /gene="torR"
                     /locus_tag="OO309_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR5848517.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6567..7739
                     /gene="torC"
                     /locus_tag="OO309_003510"
     CDS             6567..7739
                     /gene="torC"
                     /locus_tag="OO309_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR5848518.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7739..10279
                     /gene="torA"
                     /locus_tag="OO309_003511"
                     /pseudo
     CDS             7739..10279
                     /gene="torA"
                     /locus_tag="OO309_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
BASE COUNT         2488 a         2652 c         2750 g         2437 t
ORIGIN      
        1 aatgactggt ttagtaaaat ggtttaacgc agataaaggt tttggcttta tcactcctga
       61 tgatggcagc aaagacgttt tcgtccattt caccgccatc cagagcaatg aattccgcac
      121 gctgaacgaa aatcagaaag ttgaattttc tattgagcag gggcaacgtg gccccgcggc
      181 agcgaacgtt gttacgctct aaggttgccg ttattactca acatctccat ttccgctgtc
      241 catgttgtca tggttcacag taccgtacat cggcattcga tgtgacggag cgaaaccctt
      301 tgggcgctaa gtgtattttt tgtaaatcga ctatgatcac ctttgataac gtcgcgctgc
      361 aaatacgcac tgaccatgcg ccgctggatt tcacaaaata atatcaggct ccctcgtgga
      421 gcctttttta tatctgcctt atttttcttc aacgctgtat gtatagtaag cgataacctg
      481 ttgattattg aatctttcgg gaagatggct tataacattt cttacctgac cagggtaccg
      541 ggaaccaaca ccttactggc gtgttgctgt ctttcaagac cagacgaggt taacagtgaa
      601 tattgaagag ttaaaaaaac aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat
      661 cgccgagctg aacaagaata cagggaaaga agtctctgaa attcgcttca tcgcacgaga
      721 aaaaatgacc gggcttgaaa gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca
      781 tcaccccgtg cgaggtgatg ttccgcttgt tgctaattta gtgaccaatc attggcgctt
      841 gtggaattaa gcgtcggtac aattcctccg gcaccgggct ttgccatact cccgcataca
      901 ttgcgtaacc aatcaccgca aacataatcc ccagaaccag tagcgtcatt aaccagccag
      961 acaacgcaaa ggctttttta tttgctgcag gtttttgcag tgaaaaggtc aatgttgagg
     1021 ctaccggaca tgactctacg caagtcatac agccggtaca ttccactgtt cgtacctgaa
     1081 ttaatttatc taccgggatc cgtgatgggc acttttttgc gcatttgcca caatcgatac
     1141 aactttcggc attgcgacga atcttaaacg gcgacaatag cgaaaccacg cccatcagcg
     1201 cgccatatgg gcaaagataa cgacaccagg catggcgaat aaacaggctg gcaatcagca
     1261 aaacggtcac gctgattaat gtcgcggtcc ccatatgacg aaagaaatcg agcattttaa
     1321 cgtccatcac cacgctatag ggcgacaaca taaaatagtg aatcgcctga gcgggcatca
     1381 ataacgcgat atagagaaat aaactcaaca gcaaatactt cacgccgcgc agaggaatat
     1441 ccagccagcg gggaaggaca cattgccgac caaacagttt gttaccgaga tcgccgatta
     1501 attcagaaag cgtaccaacc gggcataacc atgagcaaaa ggcctttttg agtaatagac
     1561 tgatgacgat aaaagcgacc aataacagca tcgcggcggc gtggacggac ggtaactgac
     1621 ctgttgcaag gctatatttc agattcatca gaccggcaat cggtagccag ccttcgatac
     1681 ctcccggtct ggcgacaaat gtcgtactac ctgccgtttc gtaatagcgc acccaatacc
     1741 agaacgtgat ggcaatataa atattcattg ccaatagtaa gaattgcgtc gctttacgcc
     1801 aggtcgtggc attgcgccag tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc
     1861 gctgccagcg ggttcttttt ttctctgcca tgattttgcc agtccgttaa gttgcatacc
     1921 aaatgccact attctagtta ttcttaactg gctgatattg attcaaatcg cgttcaggtc
     1981 tttcttattc aatcatgctt ccagagcggc aacactgcgt gtaatttctt cgtgtggaag
     2041 gggggcagat aatggctgct gctccagttg tgcgcatagc tgactggcgc tacgcattcc
     2101 cagactcgag caactgcttt ttagctgatg cgcggcacgc tttattttct cgctgtcctg
     2161 gctggcgcgg gcaatgtcga tttcatcgag aagcggcagg gcatgttgtt taaataatgc
     2221 cagccattcg tggatcttct ccgtccccat taactgagcg tcttcattga gttgcgagac
     2281 atccagtggt agatcgttat tggcttgcag ttggagatag tgcgccagta actgaccgag
     2341 cacttcacgc ggcaccggtt tagggataat cccgcgaaat agtgaactgg tacgctggcg
     2401 cagcgtttcg tcaatgacat gggcgctaaa gccaatcaaa accagcgacg gatattgctg
     2461 tgccagttgc cgggcaaggg taatgccgtc gatatccggc agatcaaaat ccaccagtgc
     2521 ggcagcaaac ggttcgctat tttgcagtgt ctctaaagcc tgcgcggcat tgccaatagc
     2581 aacaacctgc gcaccactgg tgttcagcat ctcaacggta attcgctggg ttagcgggtt
     2641 atcttcaatt aacagcaaac gtaaaccgtc aagacgcacc gcctgattgg ctgtttttgg
     2701 cacgggtgca gtggcaacac gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg
     2761 cgtgctggtg gcgctcagtt cgccgcccat cgcctgggcc agacggctac tgatagtcag
     2821 ccccagcccg gtgccgccgc gtttgccgct tacctgcaca aatggctgga agatttctgc
     2881 cagtttcgcg ggatcaatac cgcagccgct gtcttccact tcgaccagcc attgctcgcc
     2941 atcagtgcga ctacgcagga taatgtgccc ttcgtcagta aaacgcaggg cattgctcaa
     3001 taggttggta atgacctgac gaatacgtcg tggatcgccc attaacgcgc tcggtacatc
     3061 atcggcaatt gccgttgcca ggcgaatcgg gcgacctttc acccgtccgc tcattaattg
     3121 cagggtactt tccagcagcg ggcgcggttc aaagggctca tcgctgactg aaacattttt
     3181 gccacctgct tcgatagccg aataatcgag aatatcgttg aggatggtca gcaacgattc
     3241 gccagagtcg gtaatcgcct gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc
     3301 cagcagttga gcagtgccga gaataccgta cagcggtgtg cggatctcat ggctcatcgc
     3361 cgtcagaaac gccgattttg cctggctggc tttttctgct tccgcccgtg cctgtcggtg
     3421 ttctatcacc agttcctgca attcagctgt acgcgctttg acctgcgccg ccagctgttc
     3481 gcggtggcga ttcagtgcat gaacactgct gcgaaacgca tccatcagcc gcccgatggt
     3541 atccagctcc cgtacgccag cggtttccgg gaaaggggag tcaatatcac cgtccagcag
     3601 ccgttgcagc gcctgcgttt gttcggcaag tggacgcgtg aatgagcgat aaaccacgcg
     3661 ccagaggatc ataatcagtg cgcaaagtga aaccatcccc agcaataaca ggctgtattg
     3721 cccgcgtgca ctggcttttt ccagatgtgc cagtccatgc tgattgtgca gctcaatggt
     3781 gtcgaccagc tgactgactt cgctactaaa ctgcgcgaac tgggcgatat tattttgggc
     3841 gagagtttgc aggtgattgc tgatttcact gtcctgctgg aacagcgcca acagatcgct
     3901 atattggcta acggtagtta acgttgttgc gacctgcgca cgaactcccg gatcttcaat
     3961 gcgtatttgc cgacgttgca gaattttcac cgcattattg agctgctttt ccagcgttgg
     4021 tgcatttttc tggatctgct ccagccccag attcatcacc atttgctgca cccgcagagc
     4081 gctaaggcgc agttcattca tctggttaac atactcaaga tcgatatcaa tcagccgatc
     4141 gagtgcactt tcagcagcct gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg
     4201 ggtcgctcca gcggaagttg tcgcattatt cgcctgacct tgctccaggc gtgcgatctc
     4261 atcggcggca gctatctgct gactgagttg ccgttgttgc tggcgtagtt gcagacgccg
     4321 ccccaccagt tccccttgct gacgtaatga acgggagatc tcctgctcct gttgttcaat
     4381 agcggtggta tcaaaacctt gttcccgtaa cgcttgcagc aacgcattaa tcttcaggct
     4441 ttatgcggtg agcattcgcc cctgcgcctg ccacatcttt tcgttatcgg cactggtcag
     4501 gttctgcgcg gcgaaaagtt cccaggcgct ggcttcgctc aactggcgcg ccatattcat
     4561 ggtaggaatc aatgcctgag tattgtcttt ttccacctgg ctgataaagc gcaggttgta
     4621 ccatcccacc agggtactgg tcagggttaa cagcgccatc agggcaaagc ccatccagag
     4681 tcttcgggtc agggttaaat tcacggtcgg tgcacttaag gtgaaaaagg ttgagtcgca
     4741 aagtggaatg catctagcat aaagccttat tattgatgag gctatcatgc gcgtactgct
     4801 atttttactt ctttcccttt tcatgttgtc ggcattttcg gctgataacc tgttgcgctg
     4861 gcatgatgcg cagcatttca cggtgcaagc ctctatgccg cttaaagcca aacgcgtatg
     4921 gaaactgtgc gcgctttatc ccagcctgaa agattcatac tggttatcgt tgaactatgg
     4981 tatgcaggag gctgctcgcc gctacggtgt ggatttaaaa gtgctggagg caggcggcta
     5041 cagccagttg gctacccagc aagcacaaat cgaccagtgt aaacagtggg gcgcagaggc
     5101 cattttgctc ggtagtagca cgacgtcatt tcccgacctg caaaagcagg tagcaaatct
     5161 gccggtgatc gaactggtaa atgctcttga tgatccccag gtgaaaagcc gcgttggtgt
     5221 accctggttt cagatgggct atcaacctgg gcgatatttg gtgcaatggg cgcacggtaa
     5281 accactgaac gtgctgctga tgcccggacc cgataacgcc gggggcagta aggagatggt
     5341 cgagggtttt cgcgcagcca ttgccggaag cccggtgcgt attgttgata ttgcgcttgg
     5401 tgataacgat attgaaatcc agcgcaacct gttgcaggag atgctggaac gccacccaga
     5461 aatcgacgtc gttgccggaa cggccattgc ggcagaggcg gcaatgggtg aagggcgtaa
     5521 cctgaaaacg ccgcttaccg tggtgtcgtt ttatctttca catcaggtgt atcgcgggct
     5581 gaagcgggga agagtgatta tggctgccag cgatcaaatg gtctggcagg gggaactggc
     5641 agttgagcag gccatcaggc aattacaggg gcaatcggtg tctgataatg tcagcccacc
     5701 gattttagtt ctgacgccga aaaatgccga ccgagaacat atccgccgct cgctgtcacc
     5761 ggggggattt cgtccggtct atttttatca gcacacatca gcggctaaga aataaccttc
     5821 accatgttgc gtcaccagta aatccgcgct gagtttatga cgtaaacgac gaattaacac
     5881 atcgacggtg cgcaggtcag ggttttccac ccgacgcgca gaaagcatac gcagcagacg
     5941 ctcacggctg agaatttcgc ccggattagt cacaaatgcc accaacattt catactctgc
     6001 gcgggtcagt ttaatcggct cgccatcccg ctccagcgta tggcgcgaca cattcaggca
     6061 ataacccgca aagcgatagc agtagtcctg agtgtgcggt tgagcttgtc gcgcgaggtc
     6121 gattcgccag agcagatttt tcacccgtac taccagttcg cgcagttcca gcggtttggt
     6181 gacgtaatcg tctgcgccca tttccagccc aacaatacgg tcaatccgat cgctgcgtcc
     6241 ggtaaccaga ataatcccca ccgttgagcg ttctcgcagg gcgcgggtta acatcaggcc
     6301 attttcatcg ggtaagttga tatccagcag aattaaatct accggctgat tctgcataat
     6361 ttcccgtagc ccggcaccgc tcgccgtaac ggaaacggta tacccctcct gagtgaagta
     6421 ggattgtaat cgcgcctggg taaccggctt atcttcaaca ataacaatgt gatgtggcat
     6481 cagagggttt tactcattct gttcatatct gttcatattt gctcattaag atcgcttcac
     6541 taaaccataa ttctacaggg gttattatgc ggaaactctg gaacgcgcta cgccgaccca
     6601 gtgctcgttg gtcggtactg gcgctggtcg caattgggat tgtgattggc attgcgctga
     6661 ttgtattgcc acacgttggg atcaaagtca ccagcacaac cgaattttgt gtcagttgcc
     6721 acagtatgca accggtgtat gaagaatata aacagtcggt gcatttccag aacgcctccg
     6781 gcgtgcgagc tgaatgccat gactgtcata tcccgccgga tattccaggc atggtgaagc
     6841 gcaaactgga agcgagcaac gacatttacc agacctttat tgcccactcc attgatacac
     6901 ctgaaaaatt cgaagccaaa cgcgcggaac ttgccgagcg tgaatgggcg cgaatgaaag
     6961 aaaacaactc ggcaacctgt cgctcctgcc ataactacga tgcgatggat catgccaggc
     7021 agcatcctga agcggcacgt cagatgaagg tggcagcgaa agataatcaa tcctgcatcg
     7081 actgtcataa aggtattgcc caccagttac cggatatgag tagcggcttc cgtaagcagt
     7141 tcgatgagct gcgcgccagt gctaatgaca gtggtgacac gctgtactct attgatatta
     7201 agccaattta tgcggcgaaa ggcgataaag aagcctctgg ttctctgctg cctgcttcgg
     7261 aagtgaaagt ccttaaacgt gacggcgact ggctgcaaat tgaaattacc ggctggacgg
     7321 aaagcgccgg acgtcagcgt gtactcaccc aattcccagg taaacgcatc tttgttgcct
     7381 cgattcgtgg tgatgtgcag cagcaggtga aaacgctgga gaaaaccacc gttgccgaca
     7441 ccaataccga gtggagcaag ttgcaggcca ccgcgtggat gaagaaaggc gacatggtga
     7501 acgatatcaa accgatctgg gcctatgcgg attcgttgta caacggcacc tgtaacgagt
     7561 gccacggcgc accggaaatc gcccactttg acgctaacgg ttggatcggc acgctcaacg
     7621 gcatgattgg ctttaccagt ctcgataaac gtgaagaacg cactttgttg aaatatctgc
     7681 aaatgaatgc gtctgacacc gcaggtaagg ctcacggcga taagaaggaa gaaaaataat
     7741 gaacaataac gatctctttc aggcatcacg tcggcgtttt ctggcacaac tcggcggctt
     7801 aaccgtcgcc gggatgctgg ggccgtcatt gttaacgccg cgccgtgcaa ctgcggcgca
     7861 agcggcgact gaggctgtca tctcgaaaga gggcattctt accgggtcgc actggggggc
     7921 tatccgcgcg atggtgaagg atggtcgctt tgtggcggca aaaccgttcg aactggataa
     7981 atatccgtcg aaaatgatcg ccggattgcc ggatcacgta cacaacgcgg cacgtattcg
     8041 ttatccgatg gtacgcgtgg actggctgcg taagcgccat ctgagcgaca cctcccagcg
     8101 cggtgataac cgttttgtgc gcgtgagctg ggatgaagcc ctcgacatgt tctatgaaga
     8161 actggaacgc gtacagaaaa ctcacgggcc gagtgccttg ctgaccgcca gtggttggca
     8221 atcgacgggg atgttccata acgcttcggg gatgctggcg aaagctattg ccttgcatgg
     8281 taatagcgtt ggtacgggcg gagattactc taccggtgct gcgcaggtga tcctgccgcg
     8341 cgtagtcggt tcaatggaag tgtatgaaca gcaaacctcc tggccgctgg tattgcagaa
     8401 cagcaaaacc attgtgctgt ggggctccga tttgctgaaa aaccagcaag cgaactggtg
     8461 gtgcccggat cacgatgttt atgaatatta cgagcagcta aaagcgaaag tcgccgccgg
     8521 tgaagttgag gtcatcagca tcgatccggt tgtcacatcc acccatgagt atctggggcg
     8581 cgagcatgtg aagcacattg cggttaaccc gcaaactgac gtgccgctgc aactggcgct
     8641 ggcacatacg ctgtacagtg aaaacctgta cgacaaaaac ttcctcgcta actactgtgt
     8701 gggttttgag cagttcctgc cgtatctgct gggtgagaaa gacggtcagc cgaaagatgc
     8761 cgcatgggct gaaaaactga ccggcattga tgccgaaacc attcgtgggc tggcgcggca
     8821 gatggcggcg aacagaacgc aaattattgc tggctggtgc gtgcagcgta tgcagcacgg
     8881 tgaacagtgg gcgtggatga ttgtggttct ggcggcgatg ctggggcaaa ttggcctgcc
     8941 aggtggtggt tttggctggc actacaacgg cgcaggcacg ccggggcgta aaggcgttat
     9001 tctgagtggt ttctccggct ctacgtcgat tccgcctgtt cacgacaaca gtgactacaa
     9061 aggctacagc agcactattc cgattgcccg ttttatcgat gcgatcctcg aaccggggaa
     9121 agtgatcaac tggaacggta aatcggtaaa actgccgccg ctgaaaatgt gtatttttgc
     9181 cggaactaac ccattccatc gccatcagca gatcaaccgc attattgaag gctggcgcaa
     9241 gctggaaacg gttatcgcca tagataacca gtggacctca acctgccgct ttgccgatat
     9301 cgtgctgcct gcgaccacgc agtttgagcg taacgatctc gaccagtacg gcaaccactc
     9361 caaccgtggc attatcgcca tgaaacaggt ggtgccgccg tagttcgagg cgcgcaacga
     9421 ctttgatatt ttccgcgagc tgtgccgtcg ctttaatcgt gaagaagcct ttaccgaagg
     9481 gctggacgaa atgggctggc tgaaacgcat ctggcaggaa ggtgtacaac aaggcaaagg
     9541 acgcggcgtt catctgccag cgtttgatga cttctggaat aacaaagagt acgtcgagtt
     9601 tgaccatccg cagatgtttg ttcgccacca ggcattccgc gaagatccgg atctcgaacc
     9661 gctgggcacg ccgagtggcc tgattgagat ctactcgaaa accatcgccg atatgaacta
     9721 cgacgattgt caggggcatc cgatgtggtt tgagaaaatc gaacgctccc acggtgggcc
     9781 cggctcgcag aagtatccgt tgcatctgca atctgtgcat ccggatttcc gacttcactc
     9841 gcagctatgt gagtcggaaa cgctgcgtca gcaatatacg gtagcgggta aagagccagt
     9901 gttcattaac ccgcaggatg ccagcgcgcg cggtattcgt aacggtgatg tggtacgcgt
     9961 ctttaacgct cgcggtcagg tgttggcagg agcagtagtt tctgaccgct atgcacccgg
    10021 cgtggcgcga attcacgaag gggcatggta cgatccagat aaaggcggcg agcctggtgc
    10081 gctgtgcaaa tacggtaacc ccaacgtgct gaccatcgac atcgggactt cgcagctggc
    10141 gcaggcgacc agtgcgcaca ctacgctggt ggaaattgag aagtacaatg gaacagtgga
    10201 gcaggtgacg gcgtttaacg gccccgtgga gatggtggcg cagtgcgaat atgttcccgc
    10261 gtcgcaggtg aaatcatgac cacgctgaca gcacaacaga ttgcctgtgt ttacgcctgg
    10321 ctggcgc
//
LOCUS       DAJTQQ010000122        10105 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000122 DAJTQQ010000000
VERSION     DAJTQQ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.122"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            123..254
                     /locus_tag="OO309_003512"
     CDS             123..254
                     /locus_tag="OO309_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR5848519.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            514..1509
                     /gene="pgl"
                     /locus_tag="OO309_003513"
     CDS             514..1509
                     /gene="pgl"
                     /locus_tag="OO309_003513"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR5848520.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1550..2503)
                     /locus_tag="OO309_003514"
                     /pseudo
     CDS             complement(1550..2503)
                     /locus_tag="OO309_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2687..3739
                     /locus_tag="OO309_003515"
     CDS             2687..3739
                     /locus_tag="OO309_003515"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR5848521.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3815..5248
                     /locus_tag="OO309_003516"
     CDS             3815..5248
                     /locus_tag="OO309_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCR5848522.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5431..7611
                     /locus_tag="OO309_003517"
     CDS             5431..7611
                     /locus_tag="OO309_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR5848523.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVEPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7752..9035)
                     /locus_tag="OO309_003518"
     CDS             complement(7752..9035)
                     /locus_tag="OO309_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR5848524.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(9170..10051)
                     /locus_tag="OO309_003519"
     CDS             complement(9170..10051)
                     /locus_tag="OO309_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5848525.1"
                     /translation="MTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAI
                     RRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPV
                     AATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSD
                     IVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLS
                     SGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMA
                     SQYRDDRGREWDKIEIK"
BASE COUNT         2453 a         2358 c         2551 g         2743 t
ORIGIN      
        1 tttttttctt taaaaaaaca gtacattcta ttttataatg actgtgtatt cccatggaag
       61 gagatcaccc aattctcccc cccgactttt gacgcagtgt cttgttaaca gggggattga
      121 ccatgcttat tggctatgta cgcgtatcaa caaatgacca gaataccgat ctacaacgta
      181 atgcgtttat ttgtgccgga agtgagttga ttgttgcgcg aatgggaagt tggccagaag
      241 ttgttatggc atagtgttta cggtcgttga tacaggtaaa caaaagcggg gagagtcata
      301 ctccctgaat agtcgcgaaa atatctgtgg cattgtcaaa ttcgtgtcgg tgactaatat
      361 tttagtaata tgccattcga aatggagtgc taacttattg atttaattga tgtgcgatat
      421 tgtattttat cgtgttttgt tgtctgtttt ttatccagta agtgttgtta tatgtatgaa
      481 agataaggga tttatcattt ttgaggtgtg ttcatgaagc aaacagttta tatcgccagc
      541 cctgagagcc agcaaattca cgtctggaac ctgaatcatg aaggcgcact gacgctgaca
      601 caggttgtcg atgtgccggg gcaggtgcag ccgatggtgg tcagcccgga caaacgttat
      661 ctctatgttg gtgttcgccc tgagtttcgc gtcctggcgt atcgtattgc cccggacgat
      721 ggcgcactga cctttgccgc agagtctgtg ctgccgggta gtccgacgca tatttccacc
      781 gatcaccagg ggcagtttgt ctttgtaggt tcttacaatg cgggtaacgt gagcgtaacg
      841 cgtctggaag atggcctgcc agtgggcgtc gtcgatgtag tcgaggggct ggacggttgc
      901 cattccgcca atatctcacc ggacaaccgt acgctgtggg ttccggcatt aaagcaggat
      961 cgcatttgcc tgtttacggt cagcgatgat ggtcatctgg tggcgcagga ccctgcggaa
     1021 gtgactaccg ttgaaggggc cggcccgcgt catatggtat tccatccaaa cgaacaatat
     1081 gcgtattgcg tcaatgagtt aaacagctca gtggatgtct gggaactgaa agatccgcac
     1141 ggcaatatcg aatgtgtcca gacgctggat atgatgccgg aaaacttctc cgacacccgt
     1201 tgggcggctg atattcatat caccccggat ggtcgccatt tatacgcctg cgaccgtacc
     1261 gccagtctga ttaccgtttt cagcgtttcg gaagatggca gcgtgttgag taaagaaggc
     1321 ttccagccaa cggaaaccca gccgcgcggc ttcaatgttg attacagcgg caagtatctg
     1381 attgccgctg ggcaaaaatc tcaccacatc tcggtatacg aaattgttgg cgagcagggg
     1441 ctactgcatg aaaaaggccg ctatgcggtc gggcagggac caatgtgggt ggtggttaac
     1501 gcacactaac cgctgattta accggcgcag tctctcctgc gccggtgtat taacttatct
     1561 cctgtaacgc atgtctctgg cgttcgacga tattggtcca caaattgtct ttatcgtcag
     1621 tccacaaatt aatcagcaag gaaaaaaagc gttcagctgc cggagaaagc acggcatctt
     1681 tgcggcgaat aatccccaat gtccgacgta taacgggttc caccagtggg ataccaataa
     1741 tcgaagaata gggcgcatgg ggcattgcca gaccaggaag cgccgaaatc cccagtcctg
     1801 cctccactag tcctaatgac gtcgaaagat gacgcacttc gtaaaaccag tccagcttcc
     1861 agggcttgtc ggctagctgt tgttctatca acaatcggtt gccgctggag gaacgtacgc
     1921 caatcatttt gtaacccact aactcctgcc attctacaag ctactttttg gccaatggat
     1981 ggtcacgccg acaggccagc acgaatggct cgttgaccag tggggtaaaa tcaatggatg
     2041 aatttgtgac gttgttcatg tttatgccaa agtctgattc gttacacagc accgattcca
     2101 tgcaattatt cgtgccttgt tccagaatcc gcactttaat attgggatac agctcattaa
     2161 atttaccgat tgccagcggt aaaaaataaa atactgcggt cggaatacac gctaatgtca
     2221 ccataccacg atgataagca ttcatatcac gaatattaaa aagcgtttca tcgaatttct
     2281 ttattaattc ccgcgcctcc gggagcaacc tttttcctgc ttttgttaac gtaaccttgc
     2341 gagttgtacg ctcaaatagt tgaatatgta aatcttcctc catctttttt attcttcgcg
     2401 ttaatgcagg ttgcgtaata ttgagtaatt tagcggcatt gttaaatgaa ctggactctg
     2461 ccagtatgac aaatgccttc atacttgata attcatgctt cattattact ccggaaaatg
     2521 gaagcgacga ttttgggggg ctggtcgtta aaaattttaa ctgcatttag ccaacttaaa
     2581 ttaatgaaaa aatgttatta atcgttgagc taaagtcatt agagatgctt tgtccttaat
     2641 gtaaccatat cgcaataagt tatgttttta aattgagggc attattatga aaaaaatacc
     2701 ctgcgtgatg atgcgaggtg gaacctcgag gggcgcgttc ctgttagcgg aacatttacc
     2761 cgaagatcaa acgcagcgcg ataaaatatt gatggcaatt atgggttccg gtaacgatct
     2821 ggaaattgac ggtattggcg gcggtaatcc actgaccagt aaagtcgcca ttattagccg
     2881 ttccagcgat ccgcgtgctg atgtcgatta tctgtttgct caagttatcg tccatgagca
     2941 acgtgtcgat accacgccta actgcggcaa tatgctgtct ggcgtagggg cattcgccat
     3001 tgaaaatggt ttgattgcag cgacttcgcc agttacccgt gtacgtatcc gcaacgtcaa
     3061 tacgggtacg ttcatcgaag ctgatgtgca aacgccaaat ggtgttgtcg agtacgaggg
     3121 tagcgccaga attgacggcg taccgggtac tgccgcaccg gttgcgctca ctttcctgaa
     3181 tgccgctgga acgaaaactg gaaaagtttt cccgactgat aatcagattg attattttga
     3241 cgatgtcccg gtgacctgta tcgatatggc gatgccagtc gtcattattc cggctgaata
     3301 tctgggaaaa acaggttatg aattaccggc ggaactggat gccgacaaag cattattagc
     3361 ccgcattgaa tctatccggc tacaagcggg taaagcaatg ggcttaggtg atgtcagtaa
     3421 tatggttatc cctaaacctg tgcttatttc tccagcgcag aaaggcgggg caattaatgt
     3481 gcgttatttt atgccgcatt cttgccatcg cgcgctggcg ataaccggtg ctattgctat
     3541 ttccagtagt tgtgcattgg aaggcaccgt cacccgacaa atcgtccctt ctgtaggata
     3601 tggcaatatc aatattgaac accccagtgg tgcgctcgac gttcatttaa gtaatgaagg
     3661 tcaggatgcc acgacgttac gcgcatctgt tattcggaca accagaaaaa tattttccgg
     3721 tgaagtttat cttccctgaa aaaattcgtt gtcaggataa ggacaatcaa taaaggactt
     3781 ctgtatgagt catacagaaa gaacaggatt ttaaatgaat aagaaatcgt tatggaagct
     3841 aattctgata ttagcgatcc catgtattat tggttttatg ccagctccgg caggattaag
     3901 cgaactggcg tgggtgcttt ttggtattta cctggcggcc attgtgggtc tggttatcaa
     3961 gcctttcccg gaacctgtcg tactgttaat tgccgttgct gcctcaatgg tggtggtcgg
     4021 taacttatcc gacggtgcgt ttaagaccac cgccgtatta agcggttact cttcaggtac
     4081 tacctggctg gtgttctcgg cgtttacctt aagcgccgca tttgtgacca ccggtttagg
     4141 taaacttatt gcctatctgc tgattggtaa aatcggtaac accatgctgg gtctgggtta
     4201 cgttacggta ttcctcgatc tggtactggc tccggcaaca ccgtctaaca ccgcgcgtgc
     4261 gggcggcatt gtgttaccga tcatcaacag cgtggcggtg gctttggggt ccgaaccgga
     4321 aaaaagtccg cgtcgtgtcg gacattacct gatgatgtcc atttacatgg tcaccaaaac
     4381 caccagctat atgttcttta ccgcaatggc ggggaacatt ctggcgctga aaatgatcaa
     4441 cgacattctg cacctgcaaa ttagctgggg tggatgggcg ctggccgccg gattgccggg
     4501 catcattatg ctgctggtca ccccgctggt aatttacacc atgtatccac cagaaattaa
     4561 aaaggtggat aacaaaacca tcgctaaagc gggccttgcc gaactgggac cgatgaaaat
     4621 ccgcgaaaaa atgctgctcg gtgtctttgt gctggcgcta ctgggctgga ttttcagtaa
     4681 gtctctgggg gttgatgaat ccactgtggc aatcgttgtt atggcgacca tgctgctgct
     4741 gggtatcgtt acctgggaag acgtggttaa aaataaaggc ggctggaata ccttaatctg
     4801 gtacggcggt attatcggct taagctcctt attatcgaaa gttaaatttt tcgaatggtt
     4861 agctgaagtc tttaaaaata acctggcatt tgatggtcac ggtaacgttg ctttcttcgt
     4921 tattattttc ctcagcatca tcgtgcgtta tttcttcgct tccggtagtg cctatatcgt
     4981 tgctatgtta cctgtatttg ccatgctggc gaacgtctcc ggcgcaccgt taatgttaac
     5041 cgcgctggca ctgttgttct ccaactccta tggcggcatg gttactcact atggcggcgc
     5101 ggcaggtccg gtcatctttg gcgtgggtta caacgatatt aaatcctggt ggttggtcgg
     5161 tgcggtactg acgatattaa ccttcttggt gcatatcacc ctcggcgtgt ggtggtggaa
     5221 tatgctgatc ggctggaaca tgctgtaaat atactcgtca tacttcaagt tgcatgtgct
     5281 gcgtctgcgt tcgctcaccc cagtcactta cttatgtaag ctcctgggga ttcactcact
     5341 tgtcgccttc ctgcaactcg aattatttag agtatatcca tttattatct ttctgcgcac
     5401 ttcacggtgc gcagatatct ggagcatttg atgatcaagt tatctgaaaa aggcgtgttt
     5461 ctcgccagta ataacgaaat aattgccgaa gaacatttca ccggcgaaat taaaaaagaa
     5521 gaagcaaaaa aaggcactat tgcctggtct attctctctt ctcataatac gtccggaaat
     5581 atggataaac ttaaaattaa gtttgattca ttagcctctc acgatattac ctttgttggt
     5641 attgtacaga ccgctaaagc gtccggaatg gaacgtttcc cgctgccgta tgtgctgacc
     5701 aactgccata actcactctg cgccgtcggc ggcaccatta acggtgatga ccatgttttt
     5761 ggtttatcgg cagctcagcg ttatggcggt atttttgtgc ctccgcatat tgcggtcatc
     5821 catcaatata tgcgtgagat gatggctggc gggggcaaaa tgatcctcgg gtcagacagc
     5881 cacacccgtt acggtgcatt agggacaatg gcagtcggtg agggtggcgg tgagttggta
     5941 aaacagctgc ttaatgacac ctgggatatc gactatccgg gcgtggttgc ggtgcatctg
     6001 accggaaaac ctgcgccgta tgtggagcca caggatgtgg cgctggctat cattggcgcg
     6061 gtgttcaaaa acggttacgt caaaaacaaa gtcatggagt tcgttggacc gggcgttagc
     6121 gcgctctcta ccgatttccg taacagcgtt gacgtgatga ccacggaaac cacctgttta
     6181 agctccgtct ggcaaactga tgaagaagtg cataactggc tggcgctgca cggtcgcggt
     6241 caggattact gccagcttaa ccctcaaccg atggcgtact acgatggctg catcagcgtt
     6301 gatttaagcg ccatcaaacc aatgattgcg ctgccgttcc acccgagcaa cgtgtatgaa
     6361 atcgacacac tgaaccagaa cctgaccgac attctgcgtg agattgaaat tgagtccgaa
     6421 cgcgtggcgc acggtaaagc caaactctcg ctgctggata aagtggaaaa tggtcgcctg
     6481 aaagtgcagc aggggattat cgcgggctgt tctggcggta actacgaaaa cgtcatcgcg
     6541 gcggcgaatg cactgcgcgg tcaatcctgt ggcaatgaca ccttctcgct ggcggtttac
     6601 ccgtcatcac agccggtgtt tatggatctc gccaaaaaag gtgtggtagc agatttgatt
     6661 ggcgcaggcg caatcatcag aaccgcattc tgtggcccat gctttggcgc gggcgatacg
     6721 ccaatcaaca acggtttgag tattcgccac accacgcgta acttcccgaa ccgcgaaggc
     6781 tctaagccag ccaatgggca gatgtcagcg gtggcgttga tggacgctcg ttctatcgct
     6841 gcgactgcgg caaacggtgg ctatttaacc tctgccagcg aacttgattg ctgggacaac
     6901 gtgccggagt acgccttcga tgtaacgccg tataaaaacc gtgtttatca gggctttgtg
     6961 aaaggggcaa ctcagcaacc gctgatttac ggaccgaaca ttaaagactg gccggaattg
     7021 ggtgcgctga ctgacaatat cgtcctgaaa gtgtgctcga agatcctcga cgaagtcacc
     7081 accaccgacg aactgattcc ttccggtgaa acctcttctt atcgttcaaa tccgattggt
     7141 ctggcgggga atgtcagcga gctgacagag gtgtttgcgc gcattaagca gattgctggt
     7201 caggagcata ttgatccgct gcaaactgaa attggtagca tggtctatgc ggtgaaacca
     7261 ggcgatggtt ctgcgcgtga acaggcggcg agctgccagc gtgtgattgg cggtctggcg
     7321 aatattgccg aggagtacgc gactaaacgc taccgttcta acgtcatcaa ctgggggatg
     7381 ttaccgctgc agatggcgga agtgccaacc tttgaagtgg gggattacat ttacatccct
     7441 ggcattaaag cggcgctgga taatccgggt acgacgttta aaggttatgt gatccatgaa
     7501 gatgcgccgg taacggaaat tacgctttac atggaaagcc tgacggctga agagcgcgag
     7561 attatcaagg cgggtagttt gattaacttc aataaaaacc gtcagatgta aaaagcgcca
     7621 tgtgaatgta ggtcgcattc ggcacttatt gtcggatgcg atgctggcgc atcttatccg
     7681 acctacaaat cgcatcgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcaaata
     7741 gttaatagct cttacttctt ctcctctgca accactttgc tgcccacgcc gcggttattg
     7801 tattcccaca tgcggttgta gttagtgtca ttcagattgc gctgtatttc gtcgttatca
     7861 tctacgctgc cggtattacc cgcaaacgga cgattagaga tcaccgcatc ggcccacggt
     7921 ttagccgtgt taaaaccttc gttgatggcg ctatcacgga tcaccacctg accgttggta
     7981 ttagcatcaa catccagcga gcggcccagt tgcgccacgc catcaccgga agcattgaaa
     8041 cggctgttta cggcgaggaa accgtaatag atgttagaca gcgtagccgg tgcaaacaca
     8101 tatgcttctt gctgggtacg tgagttcacc acgcggaatt cggtgttatc gaacaccact
     8161 gcgccgcgac cagaaacgat atccacatcc ccttcaatat agctgttggt caccagcgta
     8221 cgcggctgac ggttggtttc cagacggttc tgcacaccgc tgttggtgac aaagaaggtg
     8281 ttctgacgac cgagaatgtt aacgttgtta atctgtactt ggtcaccgtc agtacgcagt
     8341 gctaccgccg gatggttacc tgcatctacg ctatcgccca gcgtgttttc gatggtcaga
     8401 ttttgcagtt gcagtccatt gttttgtgac cagaagaccg cagaacacag aacaccgata
     8461 ctgtcgctgc gtttgctctg gcagctatcg tacatatacc acgctggttt acctggcata
     8521 tatttgccgc gcgggttgac gtcgtgacgc cagtcggcag ggctcatgcc cccatcaagg
     8581 gaaagcccaa tcttcacatc aatcggtttt tcacctgtac cgtacagagt gattccacct
     8641 ggagcagcag ggacatacac cgttccctga tactcaccag gcatcacggc aatatactgg
     8701 cgcttgttgg tgcgcttgat aattgccgca tctaccgccg cctgaatcgt ggtatgcgtt
     8761 acaccttgag tacccgccgg gccgacaaca aagtcaggtt gcgcaggcag ggttatcggg
     8821 gacggattcc acgctgcagc acctggtgtc agggatgcaa aatagtgttg cgcatcgaaa
     8881 ttcagcgctt cttttgccga cagaatcggg cgagaagagg taccaggcgc agtttgatca
     8941 gaaggacgtt gatcgggtgg tgttgagcta caggcggtca gcgtcacgcc aaaagccaat
     9001 gccagcgcca gacgggaaac tgaaaatgtg ttcacaggtt gctccgggct atgaaataga
     9061 aaaatgaatc cgttgaagcc tgctttttta tactaagttg gcattataaa aaagcattgc
     9121 ttatcaattt gttgcaacga acaggtcatt atcagtcaaa ataaaatcat tatttgattt
     9181 caattttgtc ccactccctg cctctgtcat cacgatactg tgatgccatg gtgtccgact
     9241 tgtgcccgag aagatgttga gcaaacttat cgcttatctg cttctcatag agtcttgcag
     9301 acaaactgcg caactcgtga aaggtaggtg gatccccttc gaaggaaaga cctgatgctt
     9361 ttcgtgcgcg cataaaatac cttgatactg tgccggatga aagcggttca cgacgagtag
     9421 atgcaattat ggtttctccg ccaagaatct ctttgcattt atcaagtgtt tccttcattg
     9481 atatcccgag agcatcaaca tgcaatgttg tagggatggc aatttttacg cctgttttgc
     9541 tttgctcgac ataaagatat ccatctacga tatcagacca cttcatttcg cataaatcac
     9601 caactcgttg cccggtaaca acagccagtt ccattgcaag tctgagccaa catggtgatg
     9661 attctgctgc ttgataaatt ttcaggtatt cgtcagccgt aagtcttgat ctccttacct
     9721 ctgattttgc tgcgcgagtg gcagcgaccg ggtttgttgt tatatggcct tcagctattg
     9781 cctctcggaa tgcatcgctc agtgttgatc tgattaactt ggctgatgcc gccttgccct
     9841 cgtctatgta tccattgagc attgccgcaa tttcttttgt ggtgatgtct tcaagtggag
     9901 catcaggcag ccccctcctt attgctttaa ttttgctcat gtaatttatg agtgtcttct
     9961 gcttgattcc tctgctggcg aggatttttt cgtagcgatc aagccatgaa tgtaacgtaa
    10021 cagaattatc actgttgatt ctcgctgtca gaggcttgtg tttgtgtcct gaaaataact
    10081 caatgttggc ctgtatagct tcagt
//
LOCUS       DAJTQQ010000123         9806 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000123 DAJTQQ010000000
VERSION     DAJTQQ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.123"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..1025)
                     /gene="bglB"
                     /locus_tag="OO309_003520"
     CDS             complement(<1..1025)
                     /gene="bglB"
                     /locus_tag="OO309_003520"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR5848526.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVG"
     gene            complement(1044..2921)
                     /gene="bglF"
                     /locus_tag="OO309_003521"
     CDS             complement(1044..2921)
                     /gene="bglF"
                     /locus_tag="OO309_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR5848527.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(3055..3876)
                     /gene="bglG"
                     /locus_tag="OO309_003522"
     CDS             complement(3055..3876)
                     /gene="bglG"
                     /locus_tag="OO309_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR5848528.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(4163..4888)
                     /gene="phoU"
                     /locus_tag="OO309_003523"
     CDS             complement(4163..4888)
                     /gene="phoU"
                     /locus_tag="OO309_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR5848529.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4903..5676)
                     /gene="pstB"
                     /locus_tag="OO309_003524"
     CDS             complement(4903..5676)
                     /gene="pstB"
                     /locus_tag="OO309_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR5848530.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5768..6658)
                     /gene="pstA"
                     /locus_tag="OO309_003525"
     CDS             complement(5768..6658)
                     /gene="pstA"
                     /locus_tag="OO309_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR5848531.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6658..7617)
                     /gene="pstC"
                     /locus_tag="OO309_003526"
     CDS             complement(6658..7617)
                     /gene="pstC"
                     /locus_tag="OO309_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR5848532.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7704..8744)
                     /gene="pstS"
                     /locus_tag="OO309_003527"
     CDS             complement(7704..8744)
                     /gene="pstS"
                     /locus_tag="OO309_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR5848533.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8992..>9806)
                     /locus_tag="OO309_003528"
     CDS             complement(8992..>9806)
                     /locus_tag="OO309_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5848534.1"
                     /translation="TSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPF
                     EYHANNPSGNTKYNCNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTIS
                     RDTPVDYSQPMADVYIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHK
                     ITKTVTIECENLGILNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVN
                     GGELPTDMDKSTVYGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         2525 a         2561 c         2268 g         2452 t
ORIGIN      
        1 aatccaaccg gatcaatttg ccacccccac tctgaacttt tcagatgcgg attggggatc
       61 atattcagta tgttgccctg cgcattttta ttaatgcttt cgtcgtggga aacacaacca
      121 gtcatgtaat aactaaaaga gatgaaatct acagtatgtt ttaaatcttc tgcgtcactt
      181 tcagtcatct caatggtgat attgtggtcg cggaagaaac gctgcatata gccgggatat
      241 tggccacgcg cctgaacatc accaaagaac atccagcgcc ggttctcttc catggcctgc
      301 aacatatcct gtggctggca ggtgaggggg taaaccagcc caccgagaag catattgccg
      361 attttcgctt cggggatcag gctatgatag gctttaactg cccgcgcact ggcaaccagt
      421 tgatgatgga tcgcctgata aacttccgcc tcgccactct cttctgccag ccccacgccc
      481 gtgaatggcg cgtgtaacga catattgatt tcattaaacg tcagccataa cgctacttta
      541 tgttggtagc gggtaaagac cgtgcgggcg taatgctcga agtgatcgat gaccgcccga
      601 ttagcccaac cgccgtagtt tttcaccagc ccatatagca tttcgtaatg ggataacgtt
      661 accagcggct tgatccccgc ctgcgccatt tcatcaaaca gccgatcgta aaacgctaac
      721 cccgcttcat tcggttcggc ttcgtcgccc tgagggaaaa ttcgcgccca ggcaatggaa
      781 atacgcagac aggtgaagcc catctcggca aataacgcga tatcttccgg gtaacggtaa
      841 taaaaatcga tggcgacatc tttgatattc tctttcccca ggatgcgcgg ttccattttt
      901 cccattacgc catgaggctg taaatctgag gtcgtgatcc ctttgccatc ttccagccag
      961 gcaccttcca cctgattggc agctgttgcg ccaccccaaa gaaatgtttc tggaaatgct
     1021 ttcataatta actcctttta tcgttagcga atgatggata acagcggttc acctgcgctt
     1081 atctgcgccg tgccgtgggg taatacgtct gtaaaatcat cgctattact gattaatacc
     1141 ggcgtcgtca gatcaaatcc ggcctcgcga atagcaggga tatcaaaaga aatcagccga
     1201 tcgcctgtat tgaccttgtc acccacgttg acgtgagcgg aaaagaattt gccgtccagt
     1261 tttacggtgt cgataccgac atgaatcagg atctccacac catcatctga ctcaatgcca
     1321 atggcgtgta atgtggcgaa caacgaagca attcgacccg caaccggaga acgcacttca
     1381 ccaaccgagg gcagaatggc gataccttta cccaacaggc cactggcaaa cgtggtatca
     1441 gcgacgtgaa tgagcggcac aatctctccc gtcatcggtg aacagatacc gccctgctca
     1501 ggtggtgtaa taacctctgg tgttttctct tgcggggcac cctgcgctgg ctgacgttta
     1561 ctggtgatga aatgaagcat caacgtacca acaaatgcgc aacctatggc aatgacaccg
     1621 ccaataacgc tggcccagac ggtgaaatca atccccgttg acgggatggt ctgcatgaag
     1681 gtgaaaatac ttggcaaacc aaaggagtaa actttcgttt gcgcgtagcc aatgatggtg
     1741 gcccccaatg ctccactgat acaggcgata acaaaggggt acttacgcgg caggttgacg
     1801 ccatataccg ctggttcggt gataccaaac agacccgtca acgccgctga tcccgccacc
     1861 acttttttct gcgcatcgcg ttcgcagagg aagacgccga gcgccgcccc gacctgcgcc
     1921 ataatggcgg gcattaacag cgggatcatg gtgtcgtagc ccagcacggt gaagttattg
     1981 atacacagcg gcaccaggcc cctgtgcagt ccgaacatga cgaagatttg ccagaagccg
     2041 cccattaccg cgcccgcaaa tgcaggaacc gcctgataaa gccagagata accggcggca
     2101 atcatttcgc ttatccaggt tgatagctgc cccaccagca gaaaggtgac gggtgtgata
     2161 accatcagac atagcaatgg tgtgaagaaa tttttgattg ccgacggtaa ccacgcatta
     2221 agtcggcgtt ccagaatgct gcacaaccag gcagaaaaaa taatgggaat aaccgatgac
     2281 gagtaattca acaatgtgac cggaataccc aggaaatcca gccccggcgc atccgctttt
     2341 tgcccgttct cgaaggcagt cagaattaat ggatgcacta acgctccacc aatcaccatg
     2401 gcagtaaatg gattaccgcc gaagcgtttg cccgcggtgt atcccaggat tatcgggaag
     2461 aacccaaaca aggcatcact ggcgctgaat aaaattaaat aagtaccact ttgttcggtc
     2521 gtccactgaa aagtgagcgc cagagccagc atacctttca ggatcccggt tgccgccatc
     2581 agaccgatca gaggcgtaaa aatacctgaa ataacataaa caaagcggtt tagcagatta
     2641 cctttctcat cattttccgg tgcctgttgc gctttttcgc caaggcctgc cacgctgtta
     2701 accgccagga agacatcggc cacatggtta cctatgacca cctgaaactg accaccgctt
     2761 tccaccacca taataatacc gggggtcttt ttcagtacct cagcttgcgc tttgctttca
     2821 tcctttaact taaaacgtaa tcgcgttgcg caatgcatca gactcacaat gttatctgcg
     2881 cccccgactc ctgcgactat ttttctggct aactccgtca taacttgccc tctaccgctt
     2941 tgcggcaaaa ctccaaaaaa aaacctgaaa aaaacggcct gacgtgaatc aagcaatttt
     3001 tttcaggttt tgcccgctta gtgcggtaac aatcctttac tcagtaataa tatttcagtg
     3061 ttctttgcgc acgcgctcta tatttatggc taaaaacata atctctgcgg gtgaaatttt
     3121 acgttgatac tgcaaaccaa taaaaatggc gatccgttcc gcacattgcc atgcttgcgg
     3181 gtaattttgt tttactgctt gttgtaatga ttcatcacta tcgttaattg aagcatgttc
     3241 aagaatacgc caggataaaa acttcagatg tgtaaccagt cgctgataac tcaagctttc
     3301 ctcctggtaa ttaaggctga actgaaattt tattaattgc agcatttcgc gcattaactg
     3361 cgtgacacct gcaacatcct ccatatttcc gctcatttgg gcactgacca gatgcatggc
     3421 aataaagccc acttcatctt tcggtaactg cacgcccaac cgtttatcaa taatggttaa
     3481 cgcttcttcc cctagctgga actctttcgg gtaaagccgc tggatatccc acagcaacac
     3541 gttttgctga aagcgtttaa tcgcaaactg gcaatggtca gttagcgaga tataaatact
     3601 gtcctgtaat tttcccaagc gctcctgcgc taaagagata agacgatcac aggttgccat
     3661 cacctcaaga ggaatatgac ttaagagttc gcttaatcgc ccgttcagtt catgactgct
     3721 caaggcatac tctttttcta ttccacttga gttaattctt tcgccagcgc gtttttgaaa
     3781 gccaattccg cgccccatga cgactttttc ccgctgttga tcatcaataa ccaccacaac
     3841 attattgttg agaattttgg tgatttgcat gttcatagca aggacctttt ttataaacaa
     3901 aaaaaaaccg acttcaccag tattctctgg ttatgtcagg ttttgcctgc gaatgcagta
     3961 acaatccagt catttattaa tggtttttat aacgaacatc caggttcgga aattaattta
     4021 attgcgtgca gagaaaatag caatgcgcta ttgataaaaa tatgaccatg ctcgcagtta
     4081 ttaactttgt gtaattttag gaatttataa agttatatat aacaaatccc aataattaag
     4141 ttattgggat ttgtctggtg aattatttgt cgctatcttt tcccgccagc agtttatcca
     4201 gctcatcacc gcctacatga cggaaatcct gacccttcac gtagtagaag ataaactcac
     4261 aaatattctg gcagcggtcg ccgatacgtt cgattgaacg cgcacagaac agcgcggtaa
     4321 gtacgctcgg aatagtgcgc gaatcttcca tcatgtaggt catcagttga cgaacaatac
     4381 cttcgtattc ctgatcgact tttttatctt cacgataaat acgtaccgct tcgtcaatgt
     4441 ccatccgcgc gaacgcgtcc agcacgtcgt gcagcatctg gatggtatga cggcccagcg
     4501 actccagact taccagcaac ggctgatgct gctgggagaa tttctccagc gcagtacggc
     4561 agattttgtc cgccacgtcg ccaatacgct ccagctcggc aatggtttta ctgatcacca
     4621 taaccagtcg caggtcgctc gccgtcggct gacgtttggc gataatgcgc acgcaggctt
     4681 catcgatcgc cacttccatc atgttgacgt tcttgtcgcc ttcgatcacg cgcttcgcca
     4741 gatcgctgtc ctggttatgc atcgcggtga ttgcatcaga aagctgctgc tccaccatgc
     4801 cgcccatggt catcacctgc gtgcggatac tttccagttc ggcgttgaac tggccggaaa
     4861 tatgtttatt aagattgaga ctgtccataa cgcactcctg aatcaaccgt aacgaccggt
     4921 gatgtagtct tctgtttgtt tcttcgctgg cttggtgaac agatcgtccg tgttgctgaa
     4981 ctcaatcaat tcgcccaggt acataaatgc cgtgtggtcg gaacaacgcg cagcctgctg
     5041 catgttgtgg gtgacgatca ccacggtgta atcctgcttc agttcggtga tcagctcttc
     5101 aatacgcccg gtagagatgg ggtcgagcgc cgaacacggt tcgtcgagca gcagcacttc
     5161 cgggcgaatg gcgataccac gcgcaataca cagacgctgt tgctgaccac cagagagaga
     5221 gtaaccgctc tggtgcaatt tatctttggt ttcgttccac aatgcggctt tggtcaatgc
     5281 ccactgcacg cgctcgtcca tgtcggcacg ggagagcttc tcaaacagac gaacgccaaa
     5341 ggcgatgttg tcgtagatgg acatcgggaa cggcgtcggt ttctggaaca ccatgcccac
     5401 tttcgcacgc agcagggcga tatcctgaga gttggtcagg atgttgtcgc catcaagcag
     5461 aatttcacct tccgcacgct gctccgggta cagttcaaac attttgttga aggtacgcag
     5521 cagcgtcgat ttaccgcagc cggacggccc gataaacgcc gtaacctggt ttttggcgat
     5581 atccaggttg atgtttttca gggcatggaa tttgccgtag tagaagttca aattacgaac
     5641 ctgaatttta ctcggggcag tttcaaccat actcattgca atcttttcct catcgtacct
     5701 gatgcttttc gctaatcagg cttaccgtag ggccgggtaa ggcgttcacg ccgcatccgg
     5761 aaaagtgtta accgtgttta ttcttcgcaa aaacaacgcg cgccagaatg ttcagcagca
     5821 ggacgcacag ggtaatgatc aataccccgg cccaggccag ttgttgccat tccgcaaacg
     5881 ggctcatcgc aaacttaaag atcgtcaccg gcaggttggc gatcggctgc atcatgtccg
     5941 tgctccagaa ctggttggag agcgcggtaa acagcagcgg cgcggtttca ccggcaatac
     6001 gggcaatcgc cagcaggata ccggtcataa tcccggacac cgacgctttc agcgtaatcg
     6061 cggagatcat cttccacttc ggtgtaccca gcgcataagc cgcttcacgc aggctgtccg
     6121 gcaccagttt cagcatgttc tcagtggtgc ggataacgat cggcacctgc aacagcgcca
     6181 gcgcaattac gcccgcccag ccggagaagt gctccatctg tgccaccacg atggtgtaga
     6241 caaacagacc aaccacaatc gacggcgcgg agagcagaat gtcgttaatg aagcgaatca
     6301 cttctgccag ccaggattta cgaccatatt ccgccagata aatccccgcc ataatgccca
     6361 gcggcgtacc gaataccgtg gcccacaaaa ttaacagccc gctacccgcc agggcgttcg
     6421 ccagaccacc accttccgta ttgggcggcg gcgtcatttc agtgaacagc gccagcgaca
     6481 taccgtcgat accgcgagtg atggtggaca ttaaaatcca gatcagccag aacaggccga
     6541 aggccatcgt cgccatcgag agcgtcagcg caatacggtt tttgaggcgg cgacgcgcct
     6601 gcattttgcg gcgagattca gccagcgccg cagtggtttg catttcaacc atagccatta
     6661 gcgtgccccc tcattcttag ccaggcgcat aatcataaac ttcgatgcgg cgaggacgat
     6721 gaaggtaatc acaaacagga tcaggcccag ttccatcagt gcggcaacgt gcagaccgga
     6781 ttccgcttcc gcaaattcgt tcgccagcgc agaggtgata ctattgcccg gcatgtacag
     6841 cgaggcgctg tcgagctggt aggtgttacc gatgataaag gtcaccgcca tggtttcacc
     6901 gagcgcgcgg cccagcccca gcatgatgcc gccgataaca ccatttttgg tgaacggaag
     6961 aacgatacgc cagataactt cccaggtggt gcagccaata ccgtaggccg actctttcat
     7021 catcaccggg gtttgttcga acacatcacg cattaccgcc gcaatgtacg gaataatcat
     7081 gatggcgagg atcacgcctg ccgcgaggat accgatacca aatgcggggc cagagaacag
     7141 cgcgccaaca atcgggatat tcgacatgat attgccgacc ggctcctgaa agtaaacggc
     7201 gaacagcggc gcaaagataa acaggcccca catgccgtaa acgatacttg gaatggctgc
     7261 cagcagctca atggcgatac ccagcgggcg tttcagccag ccaggcgcaa gctcagtcag
     7321 gaacagggcg ataccgaaac tcaccgggac ggcgatcagc agcgcgataa acgaagtcac
     7381 caacgtaccg tagatcggca ccagcgcccc gtagatatcg ttcggtgcat cccactcttt
     7441 ggtccatagg aaagccagac caaatttctg aatgctcggc caggaggaga tgatcagaga
     7501 gacaataatg ccacccaaca tcaatagcac aatcagcgcc gccagtttta ccagcacgct
     7561 gaaaattatg tcgccctttt tacccggtgg gttaaaagcg ggcttggttg cagccataag
     7621 ttactcttca gttaaacgcg tttacaaagt cattttgtaa tgccggatgc ggcgtaaaac
     7681 accgtacccg gcctggagtt ttattagtac agcggcttac cgctactgtc tttaatattg
     7741 gtcttccacg cagcgcgaac ctgttcaact acactatccg gcaggctggc gtaatccagg
     7801 tcgttcgcct gtttagcccc ggttttgtac gcccagtcga agaatttcag cacctctgtg
     7861 ccttgttctg gtttcttctg atctttgtgg atcagaatga acgtggtaga ggtaataggc
     7921 catgcatctt cgcctttctg gttggtcaga tcctgagcga aggttttgct ccagtctgca
     7981 ccttttgctg cattagcgaa gttttcttcg gtcggactaa ccggtttacc atcagcggag
     8041 atcagtttgg tgtacgccag gttgttctgc ttcgcgtaag catattcaac ataaccaatt
     8101 gcacccggca gacgctgaac gaacgcggcg ataccgtcgt tacctttacc gcccagaccg
     8161 atcggccatt ttacggtaga gccagtacca acgttgtttt tccactcttc gttcactttc
     8221 gccaggtagc tggtgaagac gaaggaagtc ccggagccat ctgcgcggcg tactacagca
     8281 atgttttgtg aaggcagttt cagacccgga ttcagtttgg cgatggcttc atcatcccac
     8341 ttcttgattt tgcccaggta gatgtcgccg agggttttac catccagcac cagttcgcca
     8401 gacttcagcc ctggaatgtt aaccgccagc accacgccgc caatcacggt cgggaactgg
     8461 aacagacctt cctgagccag tttttcgtca gacagcggcg cgtcagaggc accaaaatca
     8521 acggtattag cgataatctg ttttacgcca ccggaagaac cgataccctg gtagttaact
     8581 ttattaccgg tttctttctg gtaagtgtca gcccatttgg catacaccgg cgcagggaag
     8641 gttgcacctg cacctgtcag gcttgcttct gcaaacacag agaaagcact catcgataag
     8701 gtcgcggcga caacagttgc gacggtggta cgcataactt tcataatgtc tcctgggagg
     8761 attcataaag cattgtttgt tggctacgag aagcaaaata ggacaaacag gtgacagtta
     8821 tatataagga atatgacatt tttatgacag aaaggaatta ccaaataact tactgatttt
     8881 aaatgtgaag atgaaaattc cacatgccat ctttttgtca tgatgcattt aaaagtgata
     8941 aagaaatggg tgagatgaca acgatgtgat gaacaatcgt tgtcagacct attagcgcac
     9001 aatctcaaca gttatcgtcg ctgttgcagt aaattgcccc ggggctgggc gagctccagt
     9061 aaggctcgca ggagcagctg aaaaagttac actacctgat ttttcaccat acactgttga
     9121 tttatccatg tctgttggca actcaccacc gttcactttg atttccttat tatttttgtc
     9181 gtaaattttt atccccacgt tagagtttga agttacgacc attgaattgt ccgtattggg
     9241 ttcggtagca taaaaagacg tactgatatc atcggtattc agtatcccca ggttttcaca
     9301 ctcaatagtg actgttttag taattttatg tgttgttacc gcacttccaa caacagatga
     9361 aaaatctgca gctggtattt ctttaaaatc aaaacaaact ggctttaaat tatttatttc
     9421 gcaactttgc ggagctgtaa tatcgccccg aatataaaca tcagccatcg gctgtgagta
     9481 atccaccggg gtgtcacggc taattgttcc atacaatgtc actatctttg tttcaggaat
     9541 aatcaatttt cctgcaaatg tcttcttgat ataaaaatat actatcgctt tagccccact
     9601 ggatatactt aaaggttcaa tgcggttgca attgtacttt gtattaccag acgggttgtt
     9661 agcatgatat tcaaaaggtg cattcacata cccaacccca agaatatcta tagcaatccc
     9721 aacatcaagg ttcggattaa gatcataata attaatatta gagtgaaccc cagcataagt
     9781 taatgcctgg tttatttttg acgtat
//
LOCUS       DAJTQQ010000124         9773 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000124 DAJTQQ010000000
VERSION     DAJTQQ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.124"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            392..2023
                     /gene="oppA"
                     /locus_tag="OO309_003529"
     CDS             392..2023
                     /gene="oppA"
                     /locus_tag="OO309_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR5848535.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2109..3029
                     /gene="oppB"
                     /locus_tag="OO309_003530"
     CDS             2109..3029
                     /gene="oppB"
                     /locus_tag="OO309_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR5848536.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3044..3952
                     /gene="oppC"
                     /locus_tag="OO309_003531"
     CDS             3044..3952
                     /gene="oppC"
                     /locus_tag="OO309_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR5848537.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3964..4977
                     /gene="oppD"
                     /locus_tag="OO309_003532"
     CDS             3964..4977
                     /gene="oppD"
                     /locus_tag="OO309_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR5848538.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4974..5978
                     /gene="oppF"
                     /locus_tag="OO309_003533"
     CDS             4974..5978
                     /gene="oppF"
                     /locus_tag="OO309_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR5848539.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6031..6360)
                     /locus_tag="OO309_003534"
     CDS             complement(6031..6360)
                     /locus_tag="OO309_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR5848540.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6395..7855)
                     /gene="cls"
                     /locus_tag="OO309_003535"
     CDS             complement(6395..7855)
                     /gene="cls"
                     /locus_tag="OO309_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR5848541.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7998..8171
                     /locus_tag="OO309_003536"
     CDS             7998..8171
                     /locus_tag="OO309_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR5848542.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8226..9479)
                     /gene="kch"
                     /locus_tag="OO309_003537"
     CDS             complement(8226..9479)
                     /gene="kch"
                     /locus_tag="OO309_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR5848543.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2563 a         2286 c         2433 g         2491 t
ORIGIN      
        1 tgaaagatgt ttgttaaggc atggatgcaa gctatagatt ctgatacggt caataaaaga
       61 gaatcgctta acaattttgc aaaatgtatt ggcgagtaag aaccgcattt ggtactttcc
      121 gggcaaccgc cagacgattc tttattggta atgagaataa ttaacaatta aagagcgtcg
      181 cgaaagaata atgtgtctcg acaggggaga cacagtacga atcgacataa ggtgatcgtc
      241 tgaatcacca gaataaataa agtcggtgat agtaatacgt aacgataaag taacctgaca
      301 gcagaaagtc tccgagcttg tgcagggtcc caatccggga ttacacatgc tggttaatac
      361 cagtaattat aatgagggag tccaaaaaac aatgaccaac atcaccaaga gaagtttagt
      421 agcagctggc gttctggctg cgctaatggc agggaatgtc gcgctggcag ctgatgtacc
      481 cgcaggcgtc acactggcgg aaaaacaaac actggtacgt aacaatggtt cagaagttca
      541 gtcattagat ccgcacaaaa ttgaaggtgt tccggagtct aatatcagcc gagacctgtt
      601 tgaaggctta ctggtcagcg atcttgacgg tcatccagca cctggcgtcg ctgaatcctg
      661 ggataataaa gacgcgaaag tctggacctt ccatttgcgt aaagatgcga aatggtctga
      721 tggcacgcca gtcacagcac aagactttgt gtatagctgg caacgttctg ttgatccgaa
      781 cactgcttct ccgtatgcca gttatctgca atatgggcat atcgccggta ttgatgaaat
      841 tcttgaaggg aaaaaaccga ttaccgatct cggcgtgaaa gctattgatg atcacacatt
      901 agaagtcacc ttaagtgaac ccgttccgta cttctataaa ttacttgttc acccatcaac
      961 ttcaccggtg ccaaaagccg ctatcgagaa attcggcgaa aaatggaccc agcctggtaa
     1021 tatcgtcacc aacggtgcct ataccttaaa agattgggtc gtaaacgaac gaatcgttct
     1081 tgaacgcagc ccgacctact ggaacaacgc gaaaaccgtt attaaccagg taacctattt
     1141 gcctattgct tctgaagtta ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac
     1201 gtataacaac atgccgatcg aattgttcca gaagctgaaa aaagagatcc cggacgaagt
     1261 tcacgttgat ccatacctgt gcacttacta ttacgaaatt aacaaccaga aaccgccatt
     1321 caacgatgtg cgtgtgcgta ccgcactgaa actaggtatg gaccgcgata tcattgttaa
     1381 taaagtgaaa gcgcagggcg acatgcccgc ctatggttat actccaccgt atactgatgg
     1441 cgcaaaattg actcagccag aatggtttgg ctggagccag gaaaaacgta acgaagaagc
     1501 gaaaaaactg ctggctgaag cgggttatac cgcagacaaa ccgttgacca tcaacctgcg
     1561 gtataacacc tccgatctgc ataaaaagct ggcgattgct gcctcttcat tgtggaagaa
     1621 aaacattggt gtaaacgtca aactggttaa ccaggagtgg aaaacgttcc tcgacacccg
     1681 tcaccagggc acttttgatg tggcccgtgc aggctggtgt gcagactaca acgaaccaac
     1741 ttccttcctg aacaccatgc tgtcgaacag ctcgatgaat accgcgcatt ataagagccc
     1801 ggcctttgac agcattatgg cggaaacgct gaaagtgact gacgaggcgc agcgcacagc
     1861 tctgtacact aaagcagaac aacagctgga taaggattcg gccattgttc ctgtttatta
     1921 ctacgtgaat gcgcgtctgg tgaaaccgtg ggttggtggc tataccggca aagatccgct
     1981 ggataatacc tatacccgga atatgtacat tgtgaagcac taatggcaat acgtggggca
     2041 ggagtgtcct gctccacggt gtctgatttt tatcgcatta cagaaggcac aggccagaag
     2101 gtagggcaat gttaaaattt attctacgtc gttgtctgga agcgattccg acgctattta
     2161 ttcttattac tatttcgttc tttatgatgc gcctcgcacc gggaagccct tttaccggcg
     2221 aacgtacttt accgccagaa gtgatggcca atatcgaagc gaaatatcat cttaatgatc
     2281 cgatcatgac acagtatttc agctacctga aacaactggc gcatggcgat ttcggtccat
     2341 cgtttaaata taaagattat tcggtcaatg acctggtggc atccagtttt cccgtttctg
     2401 ccaaactggg agccgccgca tttttccttg cggtaatact gggtgttagt gctggcgtta
     2461 ttgccgcatt aaaacaaaac accaaatggg actataccgt gatggggctg gcaatgaccg
     2521 gggttgttat ccccagtttt gtggttgcgc cattattagt catgatattt gcgatcattt
     2581 tgcattggct gccgggcggt ggctggaatg gtggggcgct taaattcatg atattgccga
     2641 tggtggcgtt gtcactcgcc tatatcgcca gtattgcgcg tattacccgt ggctctatga
     2701 ttgaagtatt acactccaac tttattcgta ctgcccgggc gaaagggtta cctatgcggc
     2761 ggatcatttt acgccatgca ttaaaacctg ctctgttacc cgtgctctcc tatatgggcc
     2821 ccgcatttgt cggcattatt accggttcta tggtcatcga aaccatttat ggtttgccgg
     2881 ggattgggca actgttcgtt aatggcgcat tgaaccgtga ctattcctta gtgttaagcc
     2941 tgaccatcct ggttggtgcg ttaaccattt tgtttaatgc cattgtcgat gtgctatatg
     3001 cggttatcga cccgaaaatc cgttactgat actggagctc gcgatgatgt taagtaagaa
     3061 aaacagcgag acgctggaaa atttcagtga aaagctggag gtcgaagggc gcagcttgtg
     3121 gcaggacgca cgtcgacgtt ttatgcataa ccgtgcggcg gttgccagtc tgatagtgct
     3181 ggtgctgatc gcgttatttg taatcctggc accgatgctt tcccagtttg cctatgacga
     3241 tactgactgg gcgatgatgt ccagcgcccc ggatatggag tccggtcact actttggtac
     3301 tgactcatcc ggtcgcgacc tgcttgtgcg cgttgcgatt ggcgggcgta tctcactcat
     3361 ggtcggtgtt gctgcggcac tggtggcagt ggtcgtgggg acactttatg gttcgctttc
     3421 cggttatctg ggcggtaaag tggattcggt aatgatgcgt ctgctggaaa tcctcaactc
     3481 cttcccattc atgttcttcg tcattttgct ggtgaccttt ttcggccaaa acatcctgct
     3541 gattttcgtg gcgattggca tggtttcctg gctggatatg gctcgtattg tgcgtgggca
     3601 aaccctgagt ctgaagcgca aagagtttat tgaggcggca caagttggcg gtgtatcgac
     3661 gccgggcatt gttattcgcc acattgtgcc gaacgtactc ggtgtggtgg tggtctacgc
     3721 atcgctactg gtgcccagca tgatcctctt tgaatctttc cttagcttcc tggggttggg
     3781 tacgcaagag ccgttaagca gctggggggc attgctgagt gatggcgcga actcgatgga
     3841 agtctctcca tggttactgt tgttcccagc gggattcctt gtggtgacac tgttttgttt
     3901 caactttatc ggcgatggct tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca
     3961 gccatgagcg taattgaaac tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg
     4021 ctgaacgtga aagatttgcg tgtcaccttt agtaccccgg acggcgacgt cacggcggtc
     4081 aatgatttaa atttttccct acgtgccgga gaaacgctgg gcattgtagg tgagtctggt
     4141 tcgggtaaat cgcaaactgc atttgcgttg atgggcctgt tggctgccaa cggacgcatt
     4201 ggcggatcgg caaccttcaa tgggcgtgaa atcctcaatt tgccagagcg tgaactcaat
     4261 aaactgcgcg ctgaacaaat ctcaatgatt tttcaggacc caatgacttc gttgaatccc
     4321 tatatgcgcg tcggtgagca gttgatggaa gtgctgatgc tgcataagaa catgagcaaa
     4381 gctgaggcgt ttgaagagtc ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt
     4441 aaacgcatga aaatgtaccc gcacgaattt tctggcggca tgcgtcagcg agtcatgatt
     4501 gcgatggcat tgctatgtcg acctaagttg ctgattgcgg atgaacccac tacagcgctg
     4561 gacgtcaccg tacaggcgca gatcatgacg ctattgaatg aactgaagcg ggaatttaat
     4621 actgccatca ttatgatcac ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg
     4681 ctggtaatgt acgccgggcg cacgatggaa tatggcaatg cgcgcgatgt cttttatcaa
     4741 cccgttcatc cttattctat cggtttgctc aacgcggtgc cgcgtcttga tgcggaaggt
     4801 gaaacaatgt tgaccatccc gggtaatccg ccaaacttgc tgcgattacc gaaaggttgc
     4861 ccgttccagc cacgttgtcc acatgcgatg gaaatttgta gtagcgcacc gccgctggaa
     4921 gagtttacgc ctggccgtct gcgtgcttgc tttaaaccgg tggaggaact gttatgaatg
     4981 ctgtaactga aggaagaaaa gtcctccttg aaatcgccga tcttaaagtg cactttgaaa
     5041 tcaaagatgg caaacagtgg ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg
     5101 tcactcttcg cctgtatgaa ggggaaacat taggtgtggt aggggaatcg ggatgcggta
     5161 agtccacctt tgctcgcgcc atcatcggtt tggtcaaggc gaccgacggt catgttgcct
     5221 ggttaggtaa agagttgctg ggcatgaagc ccgatgaatg gcgtgccgtt cgcagtgata
     5281 ttcagatgat tttccaggat ccgttggcat cgctaaaccc gcgtatgacc atcggcgaga
     5341 tcatcgctga accactgcgt acttatcatc cgaaaatgtc acgccaggaa gttcgcgagc
     5401 gcgtgaaggc gatgatgctg aaagtcgggt tattgcctaa cctgattaac cgctatccgc
     5461 atgagttctc cggtgggcag tgccagcgta tcgggattgc tcgtgctctt attcttgaac
     5521 cgaagctgat tatctgcgat gagccggttt cggcgctgga cgtgtcaatt caggcgcagg
     5581 tggttaacct gctccagcag ctgcaacgtg agatgggatt gtcattaatt tttattgctc
     5641 atgacctggc cgtggtaaaa cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg
     5701 cagtagaact ggggacctat gatgaggtct accacaatcc actacatcct tacaccaggg
     5761 cattgatgtc ggcagtcccc atacctgatc cggatctgga gaagaacaaa accatccagt
     5821 tactggaagg ggaattaccg tcgccgatca acccgccttc cggttgtgtt ttccgtaccc
     5881 gttgcccgat tgccggtccg gagtgcgcca aaacacgtcc tgttctggag gggagtttca
     5941 gacacgccgt ttcttgcctg aaagtcgatc cgctttaaaa acaataaggg ctgacagttg
     6001 tcagcccttt ttcacgctaa aagcgattat ttattcccgc cagatgatat ggcaaagttt
     6061 gtgatctttt tcgcggcata acagaatgcg ggcgaaaaca tcgctgattt caccatcttc
     6121 actgtccgcc agaccaatca ccacttcggc aaaaaagtcc gggttcaaat cgaaatcaac
     6181 atgttcctgc cagtcttccg ccggatcgaa taactccgcg ccgccgcgct cttcaaactg
     6241 aagattgaac agcagaacat cagctggatc gagattgtcg gcagccagtt cgagaaaaat
     6301 atcgtaggcc tgctcaagcg tttcatcttc agtcaggcga ttgtttagat ccatatccat
     6361 agtcactacc tgtttaacct ctgttggcga cgttttacag caacgggctg aagaagtaaa
     6421 acagtcgctc ggcgacacgt tgccataatg gacgttttag ccataaacgg gcatcgagca
     6481 gacgtgaacg cgaaatataa tcgtcctgaa cggcggcaag gtctgcacca aaacctttat
     6541 cgtcgattgc caaggtaatc tcgaaattca gccacagact acgcatatca aggttaacgg
     6601 tgccaaccag acttagttcg ccatcgacca gcacgctctt ggtatgcagt aacccgcctt
     6661 caaactgata aattttaacc ccagcagcaa gcagttccgt aaagaatgcg cgactggccc
     6721 agccgaccag catcgagtca ttttttcgcg gaaggataat actgacatcc accccgcgct
     6781 gcgccgccgt gcaaatcgca tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca
     6841 tgatcaaata ttcatgcgcc gaataagccg cagtcaataa tgcctggtga atgagatctt
     6901 ccggaaagcc ggggccagaa gcaattgtgt gaatggtgtg accgctggcc tgttcaaacg
     6961 gcataatatt gacatctggt ggtggcggca gaatacgttt tccggtttca atctcccagt
     7021 cgcaggaata aataatcccc atcgcggtgg cgatggggcc ttccatacgc gccatcagat
     7081 caatccattg ccctacgccc gcatcttgtt tgaagtagcg aggatcgacc atattcatgc
     7141 tgccggtgta cgcgatgtaa ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca
     7201 tacggcgtaa aaacacacgc atcagattga cctttaaggc ttcgaccact tcaataccgg
     7261 cattacgcat tagctcgggc cacgggctgc ggaaaaaagc cacactcccg gcggagtcga
     7321 gcatcaatcg gcaatgaata ccacgccgtg cagccgccat taatgattca gccacctggt
     7381 ccgccatgcc gccgggctgc cagatataaa acaccatctc aatattatgg cgcgcgagct
     7441 ggatgtcgcg gattaacgcc tgcatcacat catctgactc ggtcatcagc tgtagctgat
     7501 tccctttgac cccagcgatc ccctgacgac gctcgcaaag cttgaataat ggcgcagcga
     7561 cactgctatt ttcttcggcg aagatatgct tacaggcttt aaggtcgtta agccattttg
     7621 cggtggaagg ccacatcgct ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc
     7681 caacggcaag ataggcaata attccgacta acggcagaat gtaaataatc aacagccagg
     7741 ccatcgcgga gggaactgcg cgtcgtttca ttagaatgcg taaagttacg cctgcaatga
     7801 gcaaccagta tcccagaatg gccaaccaac tcaccaacgt ataaacggtt gtcatagatt
     7861 agaaatcctt ttgaaagcgc attgttatga gtttacgcat atggattcat ctggcaaata
     7921 aaaacgcggg aaaagcactg gtcagcgtcg ggggcggggg tataatgacc attctgttat
     7981 tgcatagagt agttaacatg aagcggagta gaacggaagt ggggcgctgg cgcatgcagc
     8041 gtcaggctag ccgacgtaaa tcgcgttggc ttgaggggca atcgcgccga aatatgcgta
     8101 tccacagcat caggaagtgc attctaaaca aacagcgtaa ctcgttattg tttgcgatct
     8161 acaatatcta aatgtactgg gcaccattgc ggtgcccaat aagtttactc ttccgtcttt
     8221 aaactctatt tttgcgccga ttctttactg tcagctttcg tttcttgttc atcgttatcg
     8281 ctgaaaatac catgacctga gttcaacaac attgaaacga gcatatcatt attaatctct
     8341 tcaccattta aaactcgcgc cagaatttcg ctgccaaaca gttgcggtga gagaatgata
     8401 tccggatgta ccatcttaat cttattcagg tttttgctat cactgacggc gagaactgtt
     8461 ttgacatcac tgctcatatc ttttgccgag agtacaacaa acgcgttatc tgcatcgtta
     8521 tcactcagcg ccagaatggc ccggcaacga tcgattcccg ctttctttaa tactgaactg
     8581 tcattactgt caccggggat aacatcagcg ttatcgccta agcgttgctc aagttgcttg
     8641 atatcatctt caggcaagtt gctgataacc gttacgtttt gtccgcgttg attcagttgc
     8701 aggatcgtat tgatggcgag aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc
     8761 attgtatgat tgtttccttt taaaagtttg ttgaatcccc cgcggataag cggaccaaaa
     8821 attgaggtca tagatgtggc aaaaacggta atgccggaaa taatgaccga aatagtgaac
     8881 aatcgtgctg attcagaaac agggacaata tctccatagc cgacggttga catggtttct
     8941 atcgaaaaat agaacgcggt catcaaactt tctattcgcg gattaaaacc ttcgcttaaa
     9001 taaagcgcac cgtaggttga gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt
     9061 gtcccggctg cggcgctact gtgggagaag tctttgcgca gtatcagcaa aaacaccagc
     9121 gtaaaaatgc aaaatccaat actaaatttc agccagggat aaaaatgcag ggtgtaaatt
     9181 agcgctatca acaacaaaat gatactgatt gcccatgcga gctttgcccg gaacagtaag
     9241 ccaatggagt tcaggacaag aaagacgccg agcataaata gtggggcgtt tgccagcaag
     9301 gagagatcca actctgaaaa tgctttgata tggaaaatat caagcaaatt gaccgacata
     9361 ccatagattg ttttaaaaat aagcaatcca tttaaaaaga cggccagcgc gagaatgtcg
     9421 tgccgtaggg tcacccataa atttgttgct gtttgtttga atgtagccca gtgactcact
     9481 gatacctctg taaccacaat tttgtttaat tatcttagcg tgcacatttt ctgaacatac
     9541 atgcagcgcg tgaatgtgtt aaacaatagt agatgacttt atccgcagta atcaaaggtt
     9601 ttgagtgagc aactctgcgt caggaaaggg cttttttcct gaacatgctc acatcatata
     9661 aagaaataca gataaagtta ttatctgctt gtggtagtga atgcactgac cggctataag
     9721 gaaaggccaa acaagaacac ggttgcaaaa accgtgccct taaatattgg atc
//
LOCUS       DAJTQQ010000125         9743 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000125 DAJTQQ010000000
VERSION     DAJTQQ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.125"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..737)
                     /gene="pflA"
                     /locus_tag="OO309_003538"
     CDS             complement(<1..737)
                     /gene="pflA"
                     /locus_tag="OO309_003538"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR5848544.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVM"
     gene            complement(929..3211)
                     /gene="pflB"
                     /locus_tag="OO309_003539"
     CDS             complement(929..3211)
                     /gene="pflB"
                     /locus_tag="OO309_003539"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5848545.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3266..4123)
                     /gene="focA"
                     /locus_tag="OO309_003540"
     CDS             complement(3266..4123)
                     /gene="focA"
                     /locus_tag="OO309_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR5848546.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4529..6289)
                     /locus_tag="OO309_003541"
     CDS             complement(4529..6289)
                     /locus_tag="OO309_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR5848547.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6419..7045
                     /locus_tag="OO309_003542"
                     /pseudo
     CDS             6419..7045
                     /locus_tag="OO309_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7244..8332
                     /gene="serC"
                     /locus_tag="OO309_003543"
     CDS             7244..8332
                     /gene="serC"
                     /locus_tag="OO309_003543"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR5848548.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8403..9686
                     /gene="aroA"
                     /locus_tag="OO309_003544"
     CDS             8403..9686
                     /gene="aroA"
                     /locus_tag="OO309_003544"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR5848549.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2465 a         2342 c         2441 g         2495 t
ORIGIN      
        1 aacattacct tatgaccgta ctgctcaaga atgcctttca cgcgttccat ggtctctttc
       61 ttcggtggtt taacaccatc gagtttgtat tcttcaccca tcgccaccca tttgtgtttg
      121 cccagttcgt ggtaggggag aagttcgatt ttctcaacgt tacccatatc acgggtaaat
      181 tcaccgaggc gatgtgccga atcgtcatcg tcagaccagc ctgggacaac aacgtagcgg
      241 atccacacct tcacattttt gttcgccaga tatttagcga actccagcgt gcggtggttg
      301 gaaactccaa ccagattttg gtggatctcg tcgttcatct gtttgagatc gagcattacc
      361 aggtcggtta cttccagcag ttcatcaatc accggatcgt aacgacgaac aaaaccgttg
      421 gtatccagac aggtatgaat gccttctttt ttacaggcgc ggaaccagtc acgaacaaac
      481 tcagcttgca ggattgcttc accgccggat gcggtaacgc cgccgccgga agcgttcata
      541 aagtggcgat aggtcaccac ttccttcatc aaatcttcaa cggtaacttc tttaccgcca
      601 tgcgtgtccc aggtgtcgcg gttatgacaa tacaggcagc gcatcaggca gccctggaaa
      661 aaggtgataa agcgaatacc tgggccgtct acggttccac aggattcaaa ggagtgaatg
      721 cgaccaataa ctgacattgc ggtgtttctc cagatgtggc ccatctgagg ccgtgttggt
      781 gcgcagctcg aaggctacgt cgagtctgtt ttggcagtca ccttaaagta tagatagctg
      841 acaaaaaagg ctctcgtgct aaaaaaggcc ccactttcgt ggagccttta ttgtacgctt
      901 tttactgtac gatttcagtc aaatctaatt acatagattg agtgaaggta cgagtaataa
      961 cgtcctgctg ctgttcttta gtcagcgagt tgaaacgtac tgcgtagcca gatacacgga
     1021 tggtcagctg cggatatttt tccgggtttt ccatcgcgtc gagcagcatt tcacggttca
     1081 tcacgttaac gttcaggtgc tgaccacctt cgatggatgc ttcgtggtgg aagtaaccat
     1141 ccatcagacc agccaggttg gtcttacgaa cttcgtcgtc tttacccagt gcgttcggaa
     1201 cgatagagaa ggtgtaggag ataccatctt tagcgtaagc aaacggcagt ttagcaacgg
     1261 aagtcagaga cgctacagca cctttctggt cacgaccgtg catcgggtta gcacccggtc
     1321 cgaacggcgc gccagcacga cgaccgtctg gggtgttacc agttttctta ccatacacaa
     1381 cgttagaagt gatggtcaga acagactgag tcgggatagc gtcacggtag gtgtgcagtt
     1441 tctgaatttt cttcatgaaa cgttctacca ggtcaacagc caggtcatct acacgcggat
     1501 cattgttacc aaactgcggg tattcgcctt cgatttcgaa gtcgatagcc agaccgtctt
     1561 cgtcacgaat cggtttaact ttcgcatatt tgattgcaga cagggagtca gcagcaacgg
     1621 acagaccagc gataccacac gccatggtgc ggataacgtc acggtcgtgc agcgccatca
     1681 gagaggcttc gtagctgtac ttgtcgtgca tgtagtggat gatgttcagt gcagtgatgt
     1741 actgtttagc cagccagtcc atgaagtgat ccatgcgctc catcacttca tcgtagctca
     1801 ggagatcgcc tttgatcggt tcagacttcg gaccaacctg cattttcagt ttttcgtcaa
     1861 cgccgccgtt gattgcgtac agcatggttt tcgccaggtt tgcacgcgca ccgaagaact
     1921 gcatttgttt accaacgatc atcgggctta cgcagcaagc gatagcgtag tcatcgttgt
     1981 tgaagtccgg acgcatcagg tcatcgttct catactgcag agaagaggtg tcgatggaca
     2041 ctttagcggc gaatttcttg aagttcagcg gcagtttttc agaccacaga atggtcatgt
     2101 tcggttccgg agacggcccc atagtgtaca gggtgttcag gaaacggaag ctgtttttgg
     2161 taaccagggt acgaccatcg aggcccatac caccgataga ttcggttgcc cagattgggt
     2221 cgccagagaa cagttcatcg tattccggag tacgcaggaa gcgaaccata cgcagtttca
     2281 tgaccaggtg gtcaaccatt tcctgcgctt cttgttcggt gatcttgcca gctttcaggt
     2341 cacgttcgat gtacacatcc aggaaggtgg aggtacgacc gaaggacatt gcagcaccgt
     2401 tctgagactt aacagcagcc aggtagccga agtaagtcca ctggatagct tcctgagcgt
     2461 tggtagccgg accagagatg tcgtagccgt atttcgcagc catttctttc atctgaccca
     2521 gagcgcggtg ctgttcagcg atttcttcgc gcagacggat agtctgttcc aggtttacgc
     2581 cgttttccag atcagcctgt agagaggtga actgagcgta tttgtctttc atcaggtagt
     2641 cgataccgta cagcgcaacg cgacggtagt caccgatgat acggccacgg ccataagcat
     2701 ctggcagacc ggtcagaaca ccggatttac ggcaacgcag gatgtccgga gtgtaaacgt
     2761 cgaacacgcc ctggttgtga gttttacggt attcagtgaa gatttttttg atcatcgggt
     2821 ccagttcgcg gttgtacgct ttgcaggaac cttcgatcat tttgatacca ccgaacggga
     2881 taagagcacg tttcagcgga gcttcagtct gcagaccaac aactttttcc aacgctttat
     2941 tgatgtagcc agcgtcgtga gaggtgatgg tggaagcaac agcggtgtca aaatcaactg
     3001 gcgcgtgagt gcggttttcc agtttaacac cttccattac tttgtcccac agggtggtgg
     3061 tcgcttcagt agcgccagcc aggaaggact cgtcaccctc gtacggagtg tagtttttct
     3121 gaatgaagtc acggacgttt acttcattct gccagtcacc tttggtaaaa ccttcccagg
     3181 ctgtggctaa cttttcatta agctcggaca tgtaacacct accttcttaa gtggattttt
     3241 tatttactgc gtacttcgac aaccattaat ggtgatcgtt ttcacgcagg taaatgaccc
     3301 agtatgtcaa cccaaccaac aaaccaccgc cgataatatt accgatcgta accgggatca
     3361 ggttatcagt gatgaagttc atcacggtca ggtgagaaaa attttccggt gcagaaccga
     3421 cagcggtcca gaattccgga gatgcgaagt cgcggattac aatacccatc gggatcataa
     3481 acatgtttgc gatactgtgc tcaaaaccgc tggcaacaaa catcgcgacc ggcagcacca
     3541 taatgaacgc tttgtccatc aggctgcggc cagaataact catccatact gccagacata
     3601 ccatcaggtt tgccaggata ccaagacaga cggcctcaat aaaagtatgg tgcactttgt
     3661 ggtcggcggt ttgtaggacg tttagtcccc attgaccatt tgcggtcata tactcgccgg
     3721 aaagccacat taaaagtaca aacagcagtg cgccgaccag gttgccaaaa tagacattta
     3781 gccagttttt agccaactga caccaggtga tgcgcccact cgccttagca acaacaatca
     3841 acactgtgga agtaaagaga tcggctccgc agacaacaca aagaatcagc cccagagaga
     3901 agcaaatgcc gccaaccagt tttgccatgc cgaagggcat tgtgcctgtg ccagtggttg
     3961 ctgtgatata gaagacgaat gcgattgaga tgaaaacacc ggcggtaatc gccagataga
     4021 aagtcttaag cggatgtttc gttgctttat agacacccgc ctcttcggcc actttggcca
     4081 ttgcagcagg aagtaaaaga tcaaaagggt tgtcagcttt cacactaact ctctctttat
     4141 taagtcggcg acgagatact aacaaagcat tatagatgag aaattgatat agatcatatc
     4201 tcgcctggct tataggcccg taactcgcat ggtttttatg caaatacgga gtaactattt
     4261 gattatccaa ataaaaataa attttaaaaa ttaacaaatg agttgaattt tttccgcatc
     4321 ctccgctaaa acagttaatt aaaagggagc atcaggcgaa taaagtaaca atatcgatcg
     4381 tatttattaa atacaaatta ccgatattta accttataat tacaattatt ttattaatgc
     4441 aaatatatgt aaagcggggc attaaaaaaa cgccccgtaa tataactcag actaatcatt
     4501 aagcctacat tgcgtaggct atttgatttt attttgccca gaatgctgct ttggcgcgct
     4561 gcagcttttc gtaggccttc aacaacgact gatgtgcagc aaacgcgtgc agatcgctat
     4621 ctaccggttg caggccgtaa aacgccgctt cgccgctcat tgccgcactg gcggcttcta
     4681 cggcatctgc gccgtacatg cgaacaaacg cattcagata ttgcagcggc tggcgatctt
     4741 cttcctgtgc cagtaataac aacgtttgca ggcagcgata atagttggcg cgttccggac
     4801 taaatactga tgagttaaac tccatcgtcc attcggtcca gaccagagcc tgttccagat
     4861 cgccaccagc cagcgccagc atggctttta attcaccgat acgcagggtg taccaaccgt
     4921 tatccgaccc ggtcgccaga cccaacagct cacgcacgcg ggtaaagtca tcaaaacctt
     4981 cttcatccag ttgctcgatg aggttcaggt aatcttcttt ttcccactcg ctgcctggta
     5041 gcgaaagaat cgtttcacgt aaatggctgc ccatactgtt attcgcgagc cacagatctt
     5101 cagccggata aatatcggac atgccaggca cgataatacg gcaagcataa acgcccagat
     5161 gctcgtaatc ggcaatataa acttctttat cttctttgtt gaagatagcc atcagcgtag
     5221 caaactcttc ttccgtggtg ccggagaaat tccagtccac aaacggataa tcggcatcct
     5281 gcttgaacag gtcccaggag attaaaccgc tggaatcgat aaagtgcgtt tcgaggttgg
     5341 tatgttcagc gacttcttca tcatcgaagg ttggcggagt aaacacatcc aaatctttca
     5401 ggccacgacc ttgcagcagc tcggtcacgg tacgttccag tgctacgcca aaatcaggat
     5461 gcgcaccgaa agaggcaaag caggtgccgt ttgcaggatt aaacagtacc acgcaaatca
     5521 ccggatactg gccgccaagg gaaccatcat atgcgaagat cgggaaacct tctgtttcca
     5581 gtgtttcgat cgcttcaacc accgctgggt aacgcgccag cacgtctgcc gggatttccg
     5641 gcaagctgat gctttcagca ataatgcggt ttttcacgta gcgttcgaaa acttcggaca
     5701 acccctgaac gcgtgcttcg ttgcgggtat tgccagcgga cataccgtta gaaacgtaca
     5761 ggttaccaat gatattcatc ggaatataaa cggtctgatt gtcggactgg cgcgtaaacg
     5821 gcagaccgca aataccacga tcttcgttac cggattgtag gtcaatcagc atactgccgg
     5881 tcaattcatt ctccggatcg taaaatgcgc gcagacggtc atcgagcagc ccttctggca
     5941 catcgtcatt ttcgctcagt gggaaccatt tttcgttggg ataatgcaca aatggaccgt
     6001 tggcgatggt ttcgcccagc cagaaatcag caaaaaagta gttggttgag agacgctcga
     6061 aatattcacc gagtgcagaa gccagcgccg ctttcttggt tgcgccttta ccgttggtaa
     6121 aacacagtgc gcactctttg tcgcgaatat gtacagacca gacgttaggc acgggattca
     6181 gccaggaggc ctcttcaatc tgaaagccga ggtctgaaag tttttgctgg aagcgagcga
     6241 tggaatcttc cagagcggca tctttgccgg ggataaatgt ttgcgtcatg aaaatcactt
     6301 tagtcgtacg gaaagcgcgc aataatacgg gttttatctc aaaggcgcta tcaccgccgc
     6361 catgccgatg aactgttgac tatgctttta gcggataaac cacgttaaga gcataaaaat
     6421 gaaggcgttc gatctccacc gtatggcatt tgataaagtg ccttttgatt tccttggcga
     6481 agttgcacta cgtagtcttt atacctttgt actggttttt tgttcctcaa aatgaccgga
     6541 agacgcggtg tgcgacagat gtcgctgttt gaagtgttaa tcattctcac gctgggttca
     6601 gcggcgggag atgtggcgtt ttatgatgat gtgccgatgg tgccggtact tatcgtcttt
     6661 atcactctgg cgttgttata ccgcctggta atgtgatggc gagctggcct ggtcgaaact
     6721 caataactcc aacatgacgg aatttgagtt ctttatggag ctacgattgc gtggcgtgga
     6781 gcagctgggg caggtacgtc tggcgattct cgaaaccaac gggcaaatca gtgtctattt
     6841 ctttgaagat gacaaggtga aaccgggttt acttatttta cccagtgatt gtacgcagcg
     6901 ttacaaagtg gtgccggagt cggcggacta tgcctgcatt cgttgtagtg aaatcattca
     6961 tatgaacgcg ggggaaaaac aattatgtcc gcgctgtgca aatccagaat ggacgaaggc
     7021 aagtcgggca aaacgggtga cctgacagta aaaacatcgg ctttttgcta ataatccgag
     7081 agattctttt gtgtgatgca agccacattt ttgccctcaa cggttttact cattgcgatg
     7141 tgtgtcactg aatgataaaa ccgatagcca caggaataat gtattacctg tggtcgcaat
     7201 cgattgaccg cgggttaata gcaacgcaac gtggtgaggg gaaatggctc aaatcttcaa
     7261 ttttagttct ggtccggcaa tgctaccggc agaggtgctt aaacaggctc aacaggaact
     7321 gcgcgactgg aacggtcttg gtacgtcggt gatggaagtg agtcaccgtg gcaaagagtt
     7381 cattcaggtt gcagaggaag ccgagaagga ttttcgcgat cttcttaatg tcccctccaa
     7441 ctacaaggta ttattctgcc atggcggtgg tcgcggtcag tttgctgcgg taccgctgaa
     7501 tattctcggt gataaaacca ccgcagatta tgttgatgcc ggttactggg cggcaagtgc
     7561 cattaaagaa gcgaaaaaat actgcacgcc taatgtcttt gacgccaaag tgactgttga
     7621 tggtctgcgc gcggttaagc caatgcgtga atggcaactc tctgataatg cggcttatat
     7681 gcattactgc ccgaatgaaa ccatcgacgg tatcgccatc gacgagacgc cagacttcgg
     7741 caaagatgtg gtggtcgccg ccgacttctc ttcaaccatt ctttcccgtc cgattgacgt
     7801 cagccgttat ggtgtaattt acgctggcgc gcagaaaaat atcggcccgg ctggcctgac
     7861 aatcgtcatc gttcgtgaag atttgctggg caaagcgaat atcgcgtgtc cgtcgattct
     7921 ggattattcc atcctcaacg ataacggctc catgtttaac acgccgccga catttgcctg
     7981 gtatctatct ggtctggtct ttaaatggct gaaagcgaac ggcggtgtag ctgaaatgga
     8041 taaaatcaat cagcaaaaag cagaactgct atatggggtg attgataaca gcgatttcta
     8101 ccgcaatgac gtggcgaaag ctaaccgttc gcggatgaac gtgccgttcc agttggcgga
     8161 cagtgcgctt gacaaattgt tccttgaaga gtcttttgct gcaggccttc atgcactgaa
     8221 aggacaccgt gtggtcggcg gaatgcgcgc gtctatttat aatgccatgc cgctggaagg
     8281 cgttaaagcg ctgacagact tcatggttga gttcgaacgc cgtcacggtt aatgccgaaa
     8341 ttttgcttaa tccccacagc cagcctgtgg ggtttttatt tctgttgtag agagttgagt
     8401 tcatggaatc cctgacgtta caacccatcg ctcgtgtcga tggcactatt aatctgcccg
     8461 gttccaagag cgtttctaac cgcgctttat tgcttgcggc attagcacac ggcaaaacag
     8521 tattaaccaa tctgctggat agcgacgacg tgcgccatat gctgaatgca ttaacagcgt
     8581 tagggttaag ctatacgctt tcagccgatc gtacgcgttg cgaaattatc ggtaacggcg
     8641 gtccattaca cgcagaaggt gccctggagt tgttcctcgg taacgccgga acggcaatgc
     8701 gtccgctggc ggcagctctt tgtctggata gcaatgatat tgtgctgacc ggtgagccgc
     8761 gtatgaaaga gcgcccgatt ggtcatctgg tggatgcgct gcgcctgggc ggggcgaaga
     8821 tcacttacct ggaacaagaa aattatccgc cgttgcgttt acagggcggc tttactggcg
     8881 gcaacgttga cgttgatggc tccgtttcca gccaattcct caccgcactg ttaatgactg
     8941 cgcctcttgc gccggaagat acggtgattc gtattaaagg cgatctggtt tctaaacctt
     9001 atatcgacat cacactcaat ctgatgaaga cgtttggtgt tgaaattgaa aatcagcact
     9061 atcaacaatt tgtcgtaaaa ggcgggcagt cttatcagtc tccgggtact tatttggtcg
     9121 aaggcgatgc atcttcggct tcttacttcc tggcagcagc agcaatcaaa ggcggcactg
     9181 taaaagtgac cggtattgga cgtaacagta tgcagggtga tattcgcttt gctgatgtgc
     9241 tggaaaaaat gggcgcgacc atttgctggg gcgatgatta tatttcctgc acgcgtggtg
     9301 aactgaacgc tattgatatg gatatgaacc atattcctga tgcggcgatg accattgcca
     9361 cggcggcgtt atttgcaaaa ggcaccacca cgctgcgcaa tatctataac tggcgtgtta
     9421 aagagaccga tcgcctgttt gcgatggcaa cagaactgcg taaagtcggc gcggaagtgg
     9481 aagaggggca cgattacatt cgtatcactc ctccggaaaa actgaacttt gccgagatcg
     9541 cgacatacaa tgatcaccgg atggcgatgt gtttctcgct ggtggcgttg tcagatacac
     9601 cagtgacgat tcttgatccc aaatgcacgg ccaaaacatt tccggattat ttcgagcagc
     9661 tggcgcggat tagccaggca gcctgaatga acaacgggca ataaatagcc aaatctttct
     9721 ttatcaaaac gtcggcacat tgt
//
LOCUS       DAJTQQ010000126         9632 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000126 DAJTQQ010000000
VERSION     DAJTQQ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9632
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.126"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(229..1644)
                     /gene="phoA"
                     /locus_tag="OO309_003545"
     CDS             complement(229..1644)
                     /gene="phoA"
                     /locus_tag="OO309_003545"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR5848550.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1745..2005)
                     /gene="iraP"
                     /locus_tag="OO309_003546"
     CDS             complement(1745..2005)
                     /gene="iraP"
                     /locus_tag="OO309_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR5848551.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2187..2390)
                     /locus_tag="OO309_003547"
     CDS             complement(2187..2390)
                     /locus_tag="OO309_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848552.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2468..3562
                     /gene="ddlA"
                     /locus_tag="OO309_003548"
     CDS             2468..3562
                     /gene="ddlA"
                     /locus_tag="OO309_003548"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5848553.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3586..3798)
                     /locus_tag="OO309_003549"
     CDS             complement(3586..3798)
                     /locus_tag="OO309_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR5848554.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4058..4366
                     /locus_tag="OO309_003550"
     CDS             4058..4366
                     /locus_tag="OO309_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR5848555.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4425..5519)
                     /gene="yaiW"
                     /locus_tag="OO309_003551"
     CDS             complement(4425..5519)
                     /gene="yaiW"
                     /locus_tag="OO309_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR5848556.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5532..6752)
                     /gene="sbmA"
                     /locus_tag="OO309_003552"
     CDS             complement(5532..6752)
                     /gene="sbmA"
                     /locus_tag="OO309_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR5848557.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7104..8261
                     /gene="ampH"
                     /locus_tag="OO309_003553"
     CDS             7104..8261
                     /gene="ampH"
                     /locus_tag="OO309_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR5848558.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8262..8765)
                     /locus_tag="OO309_003554"
     CDS             complement(8262..8765)
                     /locus_tag="OO309_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5848559.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(8853..9380)
                     /locus_tag="OO309_003555"
                     /pseudo
     CDS             complement(8853..9380)
                     /locus_tag="OO309_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(9400..>9632)
                     /locus_tag="OO309_003556"
     CDS             complement(9400..>9632)
                     /locus_tag="OO309_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5848560.1"
                     /translation="MSRRGNCHDNAVAESFFQLLKRERIKKKIYGTREEARSDIFDYI
                     EMFYNSKRRHGSSEQMSPTEYENQYYQRLGSV"
BASE COUNT         2403 a         2453 c         2285 g         2491 t
ORIGIN      
        1 ttacaggagg tcatacgctg ttgttgtggg gttaaggttc tctcggcagc gccgacggtg
       61 gttaaaaaaa ccagaccaaa aagcaaggta accagtaatg ttattttcat agcaccatcc
      121 ctcttcatgt tttaaccatg agcgtttgcg cccgtgatct gccattaagt ctggttgcta
      181 acagcaaaaa aaccacccgg cagcgaaaat tcactgccgg gcgcagtttt atttcagccc
      241 cagagcggct ttcatggtgt agaagagatc ggtctggtcg gtcagtccaa caacattggc
      301 ggcatgcggg ccatacgccg caatacgcaa ctgactgccg gtatgttctt gtgaatcctc
      361 ttcggaattc ccgtaactca tcaccatcac tgcgccatct ttagtattta gcgcctgagt
      421 gaggcccggc gctttggtat ccggcgcaac aacctggctg gcgtgggcgt gatcagcggt
      481 gactatgacc agcgtgttac cctccttttt agcgaattcc agcgcccgtt gtacggcttc
      541 atcgagatcg accgtctcgc caatttgccc acaagggttc gcagcgtgat cctgtttatc
      601 gattgacgca ccttcaactt gcaggaaaaa tcctttctca tttttactca acaattcaat
      661 ggctttgtcg gtcatctgtg ccagggttgg tacactgtca ttacgttgcg gattaggcgt
      721 ataggtgact gcgggcttat cgatattgcc gtggtacgtt gctttcggcc ctagccagcg
      781 cactggcata ttgccgtcag caaacaggcc aagcaggggt ttttgctgat tcgcttccgt
      841 caccgaattc agtgaggcag catcgttcac caactgataa ccacgcgcct gtgcctgttc
      901 acgcagcgtt tttccctgcc attcaccagc ggttgccgtt tcagcaaagg tttttgcgcc
      961 gccgccaagc gtaacgtcgg cacgagcgtt aagcagctgt tcggtaatcg atccttttcc
     1021 gcctttttcc agagcgttac ccggacattt ttcactggtc gcgctcggac cgtagcattt
     1081 gcgcgaagtc acatgtgcca ccagcgcagc gggcgtggca tcctgcaact ctgcggtaga
     1141 aacgttaccg gtcgccagac ctgcggcttt tgccatttcc agaatcgttg ggtgatcttt
     1201 ttcgtgaata tcgacgccca gcgcgccgtt ataggttttg acatcggttg accaggcggt
     1261 tgctgatgca gccgagtcgg tgacgtagtc cggtttgcca gtttttttat tcagcgcata
     1321 gtgagtgtat tgcccggtaa gcggtaaggc atcgatacct ttaaaaaagc cgcccgcacc
     1381 ttcggcataa ttacgtgcgg cagtaatttc cgaatccccc atcccatcgc caatcagcaa
     1441 aataatattt tttgcaggtt tatcgctaag agaatcacgc agagcggcgg tctgatcacc
     1501 cgttaagcgg cgagcaccgc cgggtgcagt aatatcgccc tgagcagccc ggttttccag
     1561 aacaggcatt tctggtgtcc gggcttttgt cacaggggta aacagtaacg gtaagagtgc
     1621 cagtgcaata gtgctttgtt tcactttatt ttctccatgt acaaatacat taaaaaataa
     1681 aaacaaagcg actataagtc tcggccgtga caactttatg acagctgttg aaaagattaa
     1741 ctttttactg acaaggatgc ttcaataact tctttacgta atcgcgcagc agctctgtat
     1801 cgtcgtcagg aatgctggca tcgggcttta cctcgtacag cgccccctct acctgatcaa
     1861 tcaaccgctg ttggtcattt tgcgccatat tgcgaagcat tgcagtgacg ataatctcca
     1921 aagcttctac ctgcgcacac agttctttcg actcttcttc tttttgggca agcttaaata
     1981 acaactcagc aatgagattt ttcatgtctg tatttcctta tccaaagtat ggagaagtta
     2041 acattatgtt tcacaacttc atagtgtctg gaagtattat ttttatgaca aaggaaatac
     2101 tttgcgcaaa tattttttgt ttgattgcca gcgaaacgtt tctcttattt aaataattac
     2161 ccacacaaaa tataggcttt taattatcaa cctttattat aaataaaagc tatgaaatat
     2221 attctcttcg ttaacttata attaagagaa aaaaactcac cgcattgcca tttaagccag
     2281 gaataaatta gcttcccgtt catcattttt gtgatcttgc gcacatcttt gagataacgc
     2341 actttcttca ccagtacgtt gcacgctttg tcagcagaag attgcaacaa tctttcagaa
     2401 ggccgcgaat cccccgtttt gtcgtggtaa tgtatgcact ttgtgttaga cgggataggt
     2461 ttttaagatg gaaaaactgc gggtaggaat cgtttttggt ggtaaatcag cggaacatga
     2521 agtgtctctg caatcggcaa aaaacattgt cgatgccatt gataaaagtc gcttcgacgt
     2581 tgtgctgctg ggcattgata aacaagggca atggcacgtc agcgatgcca gcaattatct
     2641 gctaaatgca gacgatcctg cccatattgc gttgcgccct tcggcgacca gccttgcgca
     2701 ggtgccaggt aaacatgagc atcagcttat cgacgcgcaa aacggtcagc cgttgccgac
     2761 ggtggatgtc attttcccga ttgtccacgg tacgctgggc gaagatggtt ccttgcaggg
     2821 aatgctgcgg gtcgccaatt taccgtttgt aggttctgat gttctggctt cagcagcctg
     2881 tatggataaa gatgtcacca aacgtctgct acgcgatgcc gggctgaaca ttgcgccatt
     2941 tattaccctg acgcgcgcta atcgtcacaa catcagtttt gccgaagtgg agtctaaact
     3001 ggggttacca ctgtttgtaa aaccggctaa tcagggctct tctgttggtg tcagcaaagt
     3061 aaccagtgaa gaacagtacg caattgccgt cgatctggcg ttcgagttcg atcataaagt
     3121 gatcgttgag caagggatca aaggtcgtga gatcgaatgc gcagttctgg gcaacgacaa
     3181 tccgcaagcc agcacctgtg gcgagatcgt actcaccagc gatttctatg cctacgacac
     3241 caagtacatt gacgaagatg gcgcgaaagt ggtagttccg gcagccattg caccagagat
     3301 caacgataag atccgggcga ttgccgttca ggcttatcag acgttgggat gcgcaggcat
     3361 ggcgcgtgta gacgtgtttt taaccccaga gaacgaagtg gtgatcaacg agatcaacac
     3421 actgcctggc ttcaccaaca tcagtatgta tccgaagctg tggcaagcca gcggtctggg
     3481 ttacaccgat ctgatcacac gtttgattga gctggcgctg gagcgtcacg ctgcggataa
     3541 cgcattgaaa accacaatgt aatattttaa cgcccgataa tattcttatt gggcgttttc
     3601 agtttcttca tcgcgacgac gaataatcaa ccccgccagc cagaaactaa tcacccacgt
     3661 caccagaccg acggcatagg tctgccaacc ttttgcttca aatcccagta cccccaccac
     3721 gccattaaga atgaatataa ggccaatggc gaacgcatag tagtgccagt cacggcggat
     3781 ttttacaggc agattcatgg cagctccgtt aacgaaaaac aacatccttg catattctgt
     3841 aacaaccgtc tgtggtgtag acaacatttt cgtaattatt aataaattta acttaaggtt
     3901 gccattttgt gactcacaac gaagattcac gcttcgggaa tattcccaac atcaatttac
     3961 catgattctg atattgacaa tctaaatgac ctgtcagact tgccataagc cgctggtatc
     4021 acaccagaag agaagcagat gacttccgga ggtttttatg gctgatttca ccctgtcaaa
     4081 atcgctgttt agcggaaaat atcgcaatgc ctcttcaacg cctggcaaca ttgcctatgc
     4141 gttgtttgtg ctgttttgct tttgggctgg ggcgcaattg ctgaacctgt tagtgcatgc
     4201 gcccggcgtc tatgagcgtt taatgcaggt ccaggaaaca ggtcgcccac gggtggaaat
     4261 tggtttaggt gtcggcacca ttttcgggct gatcccgttt ttagcaggct gcctcatttt
     4321 tgcagtggtg gcgctatggc tgcactggcg acatcgccgc cagtaaaatt tacgttgcca
     4381 gatgcggcct gaaacgcctt atctggcctg acattcctgt ttgatcactg tttcatacaa
     4441 cgcgcccgcc gttcgtctac gcgcttcgca aaccaggccg tagtcaggtt gcgggtgatt
     4501 ttcgggcttt ccagttgaat gccaggcaac atttcgcggg gtagtgattt acccgttttc
     4561 gcttcggcaa gttgataaac tttcttgtac agcgccgtct cctcaaaaga gaagctgtca
     4621 cctttctcta actgacggcg aatttcgctg tcgctcatcc ccagttttcc cgccagtttg
     4681 cgcgttgcca gttcggtttt cccgggttct ttactgtcgt aacgaattaa atcaccgtcc
     4741 agtgccagct tcacgccgct ggccttactg actgcgttct gaaacgcggc attacggctg
     4801 gcgtaccagc cagcgttaaa atcagcaaag cggtatatcg gtgcgctata gctggcggga
     4861 taattcagta aatggtaagt accaaaccac aacccgccgc ggcgactgaa gacttcctga
     4921 cgaactgtac cgtccatttt ccacggatac cctttggtat gctgttcggc aaaagcaatg
     4981 ctgacctgca tcggcccacc ggtgcgcacc ggattaagcg aaccaaacag cgtctgcccc
     5041 attggtacca tgctgatcag atcgtcaaaa atcgcgctca attgcttttc tgtacgcacc
     5101 gaatctaacc gttcgctata acttttgccg tttggcgatt tgatttttag cgcggtatga
     5161 accagaaaag caggaatgtg catccgttca gcacgacggt caatctcttg ccaggcgatc
     5221 ttgcttaagc ccggaacggc cggatctgcc tgataatttg actcctgctg cgccaccgcc
     5281 agcaccgagt agacgttttc cagcgtcggt gccagcccct gactttcaaa ggtggtggcg
     5341 agatctttcg cccaggcatc gcggtctttc acgctcgcag gcatcttctg ccgcacgacg
     5401 ctcgctacat ctatcgcttt ttcgcccttt ttcaacggtt gcggagcctg actactacac
     5461 cccgctaaaa ccagaaccgc caacaatgac agcgaagata gatgattgac gcgcgacatt
     5521 acttctcctt tttagctcaa ggtatgggtt acttcctgaa ttttgtcacc atccagctca
     5581 tgttcaaagc tgcgcagacg tttgtagata gacatcaact caaccagtgt ggtccatgag
     5641 ttaatcaggt actggaaagc accgcgaacc tgaccaaaaa cgttggtaat ctgcgtcatc
     5701 aggccgagcg taatcgtacc ggcaacaatt gacggaaaca gcaagaacaa accgaaaacg
     5761 ttatcgacct gcaaatagag aatgcgggcg atgttgaaat acatatagtg aaaatagagg
     5821 cgaaaatagt ttttccgtac ggcgctaaac agctcgcgta ccgtaggcgg cgtcgcgcgc
     5881 gtggcatcgt cttcaccata aaccagctct ttacggtagg cggcctctac tcgctggttt
     5941 ttaaactcca gccccggcag tttgatccct accactgcca gcaatccggt ccccatcagc
     6001 gaccagacga tagcggcaat caccagacca tacggaatgt gcccgataat cggcagctcc
     6061 ggcacatgcg cggagagcgt taccagcacc ggcaggaagg cgatcaacgt catgatggcg
     6121 ttgataaaac tgacgcccat attctccagc gttgaagcaa aacgcatggt gtcttcctgc
     6181 acacgctgtg cggcaccttc gatatgacgc agttgttgcc agttcgccat gtaatattcg
     6241 ttcatcgccg tacgccagcg gaacacgtag tgactgacaa agaagttgtt cagtacactg
     6301 atcaccaccg cgatcagcgc aatccccaga aagacgccca cttcgcggta aaattgttcg
     6361 atagtgactt tatgcggcga acttagcgcg gtttgaatca gatcatagaa cggcgcatac
     6421 caggcgttga cggcgacccc gacttccacc aaaaaccaag tgacgaagat aatcagcgca
     6481 gtaccgagta ttgaccagta ttgccaacgg tgcgggctgt agataaacca gaaaaatgca
     6541 aaaagtccta cgcaaacaat gtagtaagcg taaaaaatca ggaaatccaa cgaccagaaa
     6601 cgcgcggcgc taatcgggat ctgcccggaa gcgccggtga tacgtgccac ccagtcaccc
     6661 ccaccggctt gccagaagat aacggcaatc aatgcccaaa caaaggccga gagaaaaaac
     6721 gttcccggct ttgggaaaaa agacttaaac atatgcactc ctgctaactt cttatcgttt
     6781 taacaactgc tgtgtattaa ccgcatgacc gctacaaagc gtcagccaaa tgaaaccgcc
     6841 cattgtgacc catagcaagg cgcttagttc gccagcctga ccataagaat tgtttcgacg
     6901 gatttcacaa accaaagatt gtttacataa gcggattaac acgcgcagga tcacaaatgc
     6961 aggcattggt gagcaggaaa aagatcgtgt ttacgcgccg ttacgttttt aacactatta
     7021 ttgcactaat tcgccctttg caatccatca atgagtagta taaatacgct cagttacctt
     7081 cattcaatct atggacacca ccgttgaaac gtagtctgct tttttctgcc gtgctgtgtg
     7141 cggcgtcatt gacctccgtc catgcggcgc agccgatcac tgaaccggag tttgcctctg
     7201 atattgtcga tcgttatgcc gatcatattt tttacggcag cggtgccacg gggatggcgc
     7261 tggttgttat cgacggtaac cagcgcgtct ttcgtagtta tggtgaaacg cgacctggta
     7321 ataacgttcg cccgcagctg gattcggtcg tgcgtattgc ttccctcacc aaactaatga
     7381 ccagtgaaat gctggtgaaa ttgctcgacc aggggaccgt aaagttaaac gatccgctaa
     7441 gtaaatatgc tccgccaggc gcacgcgtgc cgacctacaa tgggacgccc attaccctag
     7501 tgaatctggc aacccatacc agcgccctgc cccgtgaaca gcccggtggc gcggcacatc
     7561 gtccggtgtt tgtctggcca acgcgcgagc aacgctggaa atacctttct acggcgaagc
     7621 tgaaagccgc gccaggcagt caggcggcgt actctaacct tgcgttcgac ctgctggcag
     7681 atgcactggc gaatgcgtcc ggcaagcctt atacccagtt gtttgaagag cagattaccc
     7741 atccattggg gatgaaagac accacctaca ccccctcacc ggatcagtgc cgccgtttaa
     7801 tggttgctga gcgcggtgcc agtccgtgca ataacacgct ggcggcaatt ggcagcggcg
     7861 gcgtctattc cacgcctggc gatatgatgc gctggatgca gcagtatttg tcatctgatt
     7921 tttatcagcg cagtaaccag gcagatcgca tgcagacgtt gatttatcag cgcgcgcagt
     7981 ttacgaaagt gattggcatg gatgtccccg gcaaagcgga tgcgcttggt ttaggctggg
     8041 tatatatggc cccgaaagag ggtcgtccga ggattattca gaagacaggc ggtggtggcg
     8101 gtttcattac ctatatggcg atgatcccgc agaagaatat tggcgcgttt gtggtggtca
     8161 ctcgttcgcc gttgacgcgc tttaaaaata tgagcgatgg catcaatgat ctggtgaccg
     8221 agctaagcgg gaataaaccg ttggttatcc ccgcatcctg attaatactc tgaaggcaaa
     8281 cggttgatag cgaccagttt gcctttattc atttcgatat agccgccttt acgcaaagca
     8341 gcgagaactt ctgcgacgac agaacgcgat atgcgtgtac gttgatggat ataattcatc
     8401 acaccaatac gtgatcgcaa ttcttcattc cagtcaatca ttgataataa tgttgcgcgg
     8461 gtttgtttgg ctggtagatg atatcccgta caatttcctt ctgatattaa tttgtatggt
     8521 atatcgtttt tcattaaacc atcagccagc cccataatat aaggagcctg ggtaataccg
     8581 ataagtacgt tttcttctct acgcagagag ataactcctt ctaaaataac gaaagtatct
     8641 tcgttattga catcactgga aaatataact tgcttttcat tattaaactc gaagcgcgta
     8701 ccgtatctgg acaaacattt atcgagctta ccaaattcct gaagaggttt aactacagat
     8761 aacatttgcg cgtcctttgc agtaatgccc gtcaaatcct tgacgggcat tatttagatt
     8821 aaattaccag tatttcttcg gagtgaagaa tattaccaag tatatttaac acccacgttc
     8881 gcggaccagt cttgatctac gtcaccacca ccgaggtagt tagcatcggt ataggcgctg
     8941 aagttcttgg tgaagctgaa ctgagtaccc agaccaacac gtaccgcaga cccttcagta
     9001 ccgttatcga tggaatcacc gttcacatcg ttatcgttgt tagagtcgtc gtagacgtaa
     9061 gccagtttga agtacggagt cagagcctga tcttcgctgt aggtgaaggt ataacctgca
     9121 tctacaccca gttcataacg catgctgtcg taaggctgac cgtcaacttt catgtcgttg
     9181 ctcagctggt agtcatcacc agactggaac agaccagaaa tgctgccgta aggagtcacg
     9241 taaccagcat cacccagttt gaagtcgtaa ccggttttca aaccgaagcc ccaagcgtcg
     9301 gagttggtgc taccgtcaac gtaagtaccg ttgctcatgg ttgcagacag gtcgttgttg
     9361 aagtgagagt agctcaagct atgaatcgcc acggataatc tagacacttc cgagccgttg
     9421 ataatactgg ttttcatatt ctgtcggtga catctgttcg ctagaaccat gccgacgctt
     9481 actgttataa aacatttcga tgtaatcaaa aatatcactg cgggcttctt cccgcgttcc
     9541 gtagatcttt ttctttatcc gttcacgttt caacaactgg aaaaaacttt ctgcaaccgc
     9601 attatcatgg cagttaccgc gacggctcat gc
//
LOCUS       DAJTQQ010000127         9528 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000127 DAJTQQ010000000
VERSION     DAJTQQ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.127"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            383..1501
                     /locus_tag="OO309_003557"
     CDS             383..1501
                     /locus_tag="OO309_003557"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5848561.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1567..1815
                     /locus_tag="OO309_003558"
     CDS             1567..1815
                     /locus_tag="OO309_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR5848562.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1880..2248
                     /locus_tag="OO309_003559"
     CDS             1880..2248
                     /locus_tag="OO309_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5848563.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2342..2995
                     /gene="nfsB"
                     /locus_tag="OO309_003560"
     CDS             2342..2995
                     /gene="nfsB"
                     /locus_tag="OO309_003560"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR5848564.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3103..4350
                     /gene="ybdG"
                     /locus_tag="OO309_003561"
     CDS             3103..4350
                     /gene="ybdG"
                     /locus_tag="OO309_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR5848565.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="OO309_003562"
     CDS             complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="OO309_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR5848566.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="OO309_003563"
     CDS             complement(5895..9038)
                     /gene="cusA"
                     /locus_tag="OO309_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR5848567.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKQLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9050..>9528)
                     /gene="cusB"
                     /locus_tag="OO309_003564"
     CDS             complement(9050..>9528)
                     /gene="cusB"
                     /locus_tag="OO309_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCR5848568.1"
                     /translation="IPESIAWLVKDASQFTLTVPARPDKTLTIRKWTLLPGVDAATRT
                     LQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALIDTGNEQRVITVDADGRFV
                     PKRVAVFQASQGVTALRSGLAEGEKVVSGGLFLIDSEANISGALERMRSESATHAH"
BASE COUNT         2325 a         2643 c         2322 g         2238 t
ORIGIN      
        1 gttgatagaa atatctcctg gggcttcatc tttccagacc tcagagtaaa cctgaaatcg
       61 gaaagcttca ggcacccgca gttatcttac ccttcctcaa caaaatagct gtttatttat
      121 acagcaaatc ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt
      181 tttttctgca tgcctcaatc gtcaaaattc ttcgccacca gccagctctg ccataacttg
      241 ccacattgaa aaagtagcca aagtgcttca ggcaccatca gttcaccatc accaaaaaaa
      301 agaaacattc cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa
      361 aacgtcaact taagagaaac ctatgccatt acccgatttt catgtttctg aaccttttac
      421 cctcggtatt gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga
      481 ctcttcaatg ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga
      541 tatcaccgaa agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc
      601 cgggcagttt tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga
      661 aatttgcggc ggtggcacgc ccccgtttca gaaatggcag cgtcaggagg tatgcgacaa
      721 cgaacgctat caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt
      781 tggtcagcat gtccatgttg gctgtgccag tggcgatgac gccatttatt tgctgcacgg
      841 cttgtcacgg tttgtgccgc actttattgc cctttccgcc gcgtcgccat atatgcaggg
      901 aacggatacg cgttttgccc cctcacgacc gaatattttt tccgcctttc ctgataatgg
      961 cccgatgccg tgggtcagta actggcaaca atttgaagcc ctgtttcgct gtctgagtta
     1021 caccacgatg atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt
     1081 tggcacggtg gaggttcggg tgatggatac cccgttaacc cttagccacg cggtaaatat
     1141 ggcgggatta attcaggcca ccgcccactg gttactgacg gaacgcccgt tcaaacatca
     1201 ggagaaagat tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atggtctgga
     1261 aggcgtcatt accgatccgc acactggcga tcgtcgaccg ctaacggaag ataccttgcg
     1321 attgctggaa aaaatcgccc cttcagcaca taaaattggc gcatcgagcg caattgaagc
     1381 actgcatcgc caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc
     1441 cgatggcggc tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccggtgacta
     1501 agccgggaat tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac
     1561 aatgttatga aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct
     1621 ttcctctctt gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg
     1681 gtgaccatgt tccatctgat ggatcttagc cagctttaca ctttattgtt ttgtctgtgg
     1741 tttttagtgc tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc
     1801 tcgctggcgg attaaacgtc gattatcgcc gaacttcgcg gcatactttg cttattctcc
     1861 ttcgcctaaa ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctagc
     1921 tcttgagtta ccctttgtcg agctttgctg gccttttggc ccggagttcg atgtttttaa
     1981 aattggcggc aagattttta tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct
     2041 gaagtccgat ccacaaaaat ccctgttaaa tcagcaaata tacccaagca ttaagccagg
     2101 gtatcacatg aataaaaagc actggatttc ggtgtattcc ggcgaggaaa tctccgaagc
     2161 gttacttcgc gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg
     2221 cgatcaaaaa agagtgcgtc caggctaaag cgggaatcta tagcgcattt ttctcgctta
     2281 ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa ttacttacac atggagtctt
     2341 tatggatatc atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa
     2401 aaaacttacc ccggaacagg ccgagcagat caaaacgcta ctgcaataca gcccatccag
     2461 caccaactcc cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt
     2521 tgccaaatcc gctgccggta attatgtgtt caacaaacgt aaaatacttg atgcctcgca
     2581 cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga
     2641 ccaggaagat gccgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg
     2701 tcgcaagttc ttcgcctata tgcaccgtaa agatctgcat gatgatgcag agtggatggc
     2761 aaaacaggtt tatctcaacg tcggtaactt cctgctcggc gtggcggcgc tgggtctgga
     2821 cgcggtgccc atcgaaggtt ttgacgccgc aatcctcgat gcagaatttg gtctgaaaga
     2881 gaaaggctac accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa
     2941 cgctacgctg ccgaaatctc gtctgccgca aaacaccacc ttaaccgaag tgtaattctc
     3001 tcttgccggg catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt
     3061 ttcagccgtc atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc
     3121 caggttgaag atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc
     3181 ggtattattt ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg
     3241 accttcgaaa aacgtgccat cgccagttca cggctttggt tgcaaatcat tacccagaat
     3301 aaactcttcc accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta
     3361 ttctggctgc aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg
     3421 atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg
     3481 cagaaattcc cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg
     3541 atcggcgcaa tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg
     3601 attctaatca gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg
     3661 attcttggtc tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac
     3721 tggctggaaa tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc
     3781 gtcaaagtgc gtaactggga caataccatt accactattc ccacctggtc tctggtttct
     3841 gactccttta aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt
     3901 atcagtattg atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat
     3961 aaagcgcatt tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat
     4021 cgccagcaag gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc
     4081 tttcgtgcct atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc
     4141 ttaatggtac gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt
     4201 actaacaccg tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata
     4261 tttgcgattg tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt
     4321 cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga
     4381 gagcggacat cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa
     4441 atgccacaaa caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat
     4501 ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga
     4561 acggatagag cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gccgcgcgaa
     4621 aacgcagatg cgccaggcag atcataatcc agttcaacag caacgttgct accaccagcg
     4681 ccatcagcag accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag
     4741 tgatcgctcc ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca
     4801 aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa
     4861 ccccgctgtt atacaccgac agcgatgcca ctagaatgac gaagttcagc gcagaagcta
     4921 ccacattgct gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt
     4981 ccacccacgg atagagtgcc agtaaaacca ccagtgaacc gatataaaac agcaggatgc
     5041 gatacaccac ctgatttacc gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg
     5101 ctgtaatccc aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa
     5161 tcagcccatt ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg
     5221 ctttctcgcc gccgtaacca gaaaacagca gccacaggcc aaagccgatc ataccgatga
     5281 tcgccagcac tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca
     5341 ccaggttaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat
     5401 ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca
     5461 cgaacattac ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat
     5521 aggcaaagtg ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc
     5581 gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg
     5641 ccatctgaat cgccgggcca atgccaagaa acagaccagt accaattgcg ccacccagcg
     5701 caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg
     5761 cagtatcttc cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt
     5821 tgaggggcac cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa
     5881 cgaggtatcc tgctttaatt ccgtacccga tgtcggtgca gccacatcag cttatacgcc
     5941 gccgggataa taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggtgcg
     6001 gcaatccggc tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg
     6061 gcgataatca ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc gccgtgatac
     6121 aacgcctcat ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggctcggcc
     6181 tctatggcgt gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggca
     6241 agggcaataa agccggtacc cgtcgccaca gaaagatgaa agcccatcca ccacagtaac
     6301 cagatgccgc ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc
     6361 cgacggaatg ccagatacaa cagcacgaag atgatcatca gtgtcatcgg caccatgagt
     6421 ttcagcttat ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg
     6481 cccggtttta actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc
     6541 atgtcacgat cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc
     6601 agcatcgacg gtccggtaga gactttaacg tcggccacgt ctgccagggt gatttgctgc
     6661 ttcatcggcg tcaggatcgg caactggcgc agtgcctgcg gactatcgcg ccagctttgc
     6721 ggataacgca gattaattgg ataacgggca atcccttcca ccgtttcgcc aaccatcgcc
     6781 ccgcccaccg cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg
     6841 gctttttcac ggttaatctc aacgttgata tagcgcccac cttccagccg ctcagcaagc
     6901 gcagaagcta cgcctggcac cgttcgcgcc acttcttcaa tttgctcagc catcgcgtcg
     6961 atatccgcca gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag
     7021 agcatatcga tacggttacg aattggcggc acccacagat tcgccagccc cggcagacgc
     7081 acggtgttat ccagttcctc aatgattttg tccattgtca tgcctggccg ccactgatcc
     7141 tgtggcttaa gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt
     7201 tccgctttcc cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg
     7261 tcggtttttt gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc
     7321 atatacaaca agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc
     7381 ggccagagaa ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc
     7441 agtactttca gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cggattactg
     7501 ctttccggcg gaattttgcc acggatccag taacccatca ggatagggat cactacgatc
     7561 gccagcagcg ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga
     7621 cgcccttcct gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga
     7681 ctgataaaca gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt
     7741 ttattatcca gcgtggcgtc aggatgctgg tgctgccact cttccagctg tttatgcgca
     7801 ttctcgatca tgacgatagc ggcatcgacc atcgccccga cggcaatcgc aatgccaccc
     7861 agcgacataa tattggcatt cagcccctgg aagggcatga caataaaagc aatacacaac
     7921 cccagcggca acgaaataat cgccaccagc gccgagcgca catgccagag aaacggcgcg
     7981 cagactaccg ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcc
     8041 cggtcaatga gctggctgcg gtcgtatgtt gtaactatct ccacgccttc cggcagacta
     8101 cttttcagcg tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg
     8161 gatcgcagga tcaccacccc gcccgccact tcgccttcgc cgtttagttc ggcaatgccc
     8221 cggcgcatct tcgggccaat ctggaccttc gcaacatcgc gcagataaac gggcacgcca
     8281 ttttcactgg cttttaaaac gatgtgatta aagtcgtcga gcgtttgtag atagccgctg
     8341 gcgcgcacca tatattctgc ttccgccagt tcgattgacg aaccgcccgc ttcctggttt
     8401 gaagcatcca gcgcgctttt tacttcggcg agactgatgc catactgcgc caggcgctga
     8461 ggatcgataa ccacctgata ctctttcacc acaccgccta ccgacgccac ttccgcaacg
     8521 tcaggaatgg ttttcagctc atatttgaga aaccagtcct gcaatgagcg taaatcggca
     8581 agatcgtgct taccgctgcg atccaccagt gcatattcat agatccagcc aacacccgtg
     8641 gcatctggtc ccagctcggc gctgactccc gcaggcagct taccctgtac ctggttgagg
     8701 tactccagca cccgcgaacg cgcccagtac ggatcggtgc catcttcgaa aatgacatac
     8761 acataagagt cgccaaactg cgagaaacca cgcacagtct tcgcgccagg caccgacaac
     8821 atggtggtgg ttagcggata agtcacctga ttttcaacga tttgcggtgc ctggccggga
     8881 tagctggttt taataatcac ctgcacatcg gagagatccg gcaacgcatc cactggcgta
     8941 ttaatgatgg tccaggtgcc ccagatgctc agaaacaacg cgcccatcag caccaaaaaa
     9001 cggttcgcca ccgagcgacg aataatccat tcaatcattg gttattccct caatgcgcat
     9061 gggtagcact ttcagagcgc atccgctcca gtgcgccaga aatattggct tcggaatcaa
     9121 tcaggaacag gccgccggaa accacctttt caccttccgc cagaccagag cgtaatgcgg
     9181 tgacgccttg cgatgcctgg aaaacagcaa cgcgtttcgg tacaaagcgc ccgtcggcat
     9241 caacggtaat cacccgctgt tcgttgccgg tatcaatcag cgcctgtgac ggaatgagca
     9301 gcatcggttc gctggcggtg ttgagttgca accaggcgtt catgcccggt tttagcgcct
     9361 cgtcggcgtt gtcgacttcc agacgcagct gcagcgtgcg ggtcgcggca tccacgccag
     9421 gtagcagcgt ccatttgcgg atggtgagtg ttttatccgg tcgcgccgga acggtgaggg
     9481 taaactgcga ggcatctttc accagccagg cgatggactc cgggatcg
//
LOCUS       DAJTQQ010000128         9404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000128 DAJTQQ010000000
VERSION     DAJTQQ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.128"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..594)
                     /locus_tag="OO309_003565"
     CDS             complement(<1..594)
                     /locus_tag="OO309_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HCR5848569.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQN"
     gene            complement(661..1386)
                     /gene="fimC"
                     /locus_tag="OO309_003566"
     CDS             complement(661..1386)
                     /gene="fimC"
                     /locus_tag="OO309_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5848570.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1423..1962)
                     /locus_tag="OO309_003567"
     CDS             complement(1423..1962)
                     /locus_tag="OO309_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5848571.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2027..2575)
                     /gene="fimA"
                     /locus_tag="OO309_003568"
     CDS             complement(2027..2575)
                     /gene="fimA"
                     /locus_tag="OO309_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5848572.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(3056..3652)
                     /gene="fimE"
                     /locus_tag="OO309_003569"
     CDS             complement(3056..3652)
                     /gene="fimE"
                     /locus_tag="OO309_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR5848573.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(4130..4732)
                     /gene="fimB"
                     /locus_tag="OO309_003570"
                     /pseudo
     CDS             complement(4130..4732)
                     /gene="fimB"
                     /locus_tag="OO309_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            6186..6902
                     /gene="nanC"
                     /locus_tag="OO309_003571"
     CDS             6186..6902
                     /gene="nanC"
                     /locus_tag="OO309_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR5848574.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            6922..8028
                     /gene="nanM"
                     /locus_tag="OO309_003572"
     CDS             6922..8028
                     /gene="nanM"
                     /locus_tag="OO309_003572"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR5848575.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            8093..9073
                     /locus_tag="OO309_003573"
     CDS             8093..9073
                     /locus_tag="OO309_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR5848576.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2751 a         2054 c         1977 g         2622 t
ORIGIN      
        1 attctgtaca ctatttccgc tgaaattata attgagcaat ccggcattaa taccgggatc
       61 ccataactca ggaggaatat aaccacgcgc gcgattactc ataaatgcct gagggatcgt
      121 caggttcagt cgctgctgac caacatccag atgcgcagta gcgtcctgga ccattgtggt
      181 taatggcaca caggcatcat ccgccagcag attcataccg gcgacagaag ccgtattcag
      241 ccccatactg gcgagttgcg cgcgtgtcag gcagggaaca atcccttgtt cactgtcgcc
      301 cgtattaaat gtgacatcac gcgttgccat ataaccatta ttcaaataga tatcgacgcg
      361 atacgtccct ggcggtaatt cttgcccatt ttcaaaacgc gataaatcgg ccacagcctg
      421 gggatcatcc gctaaaaagc gcagattaaa atagaggtcg gcagatgaca aaggtgcctg
      481 tgcggcaaaa gcacaggcga caacgagtcg gacaaaaaaa ccagccaaac gatgcttacg
      541 aatatgcaag cattgtgtgt ttcgctggta aagtcttaaa ttcagatatg acattattcc
      601 tccggtttaa aatcacccgg cagtcaattc tttttattca ggcgaaaaat tccccctgcg
      661 ttattccatt acgcccgtca ttttgggggt aagtgcgcca taatcattta ttgttcggta
      721 agtaatattg cttcctgcat cagaaggcaa tttaaccgtg ctttcgccca ttggaggcac
      781 caatgcattt tcaagaaccc gggttccggc attcaactct gttaccgtca ggtaataggg
      841 tgtcgggtta atcagcgtca gagaattcgc gctacgacga aatcttaatt tttctgcggc
      901 ctgatcgggt ggcaacgcta atttagccgg gcgatagtac agtttaatgc ggctgataat
      961 tgcgagctgt agcatattct cagtcaattt tgatttatcc attgacggaa tcgctttaac
     1021 gttcatccag aataaacttt cccggtcctg tggcaattgg ttatttgttg catcaagaat
     1081 acgtaaggta ttctcttttt ttcccttcat cgcaaacaga ggaggcgtca cgataaaacg
     1141 accatccttt acaccatcgg cattttccac ccatgattga attaaatagg tactattttc
     1201 atcattattt gtcacggcaa gttgcacttg tttttgccct gccggataaa ttacgcgagt
     1261 cgcacctaag gccactcctg cttcagcgcg tccggcaacc atcattgcca tgaacatcag
     1321 gatacctgcc agcaagcaga atgttatttc ctgcgatttc cttacattga cgtttttatt
     1381 actcactgtc ctgttcctgt tatcacatta tctactgaac aattactgat aggttaaaga
     1441 gaaccaggcc tgggcattgg cgatgccgcc agtaacccga cgcccggtag cacgatattt
     1501 ggcgatgaaa tgtagcgaag tagagcctga gtaaagccgt ttccagtttg ctggaggacg
     1561 attaatcggt acgaggtttc cttcatcatc aaacaacgct acaccaatat tggaggctat
     1621 ccctggcccc tctcccacgg agagcacatc cggattttta ccatccgcga caccgtgaaa
     1681 cgccacaccc acacgttcac tcaccaccgt gctacattcc cgtaaatgaa taacaaaagg
     1741 caccggtgcg ctatcttccc ctgccgcatg aaaccggtta ctgctgattt gccccatatt
     1801 gaccgtcatt tgtttatcac cggcttcaat ccggcaagtt tccgcaataa tgacgccctg
     1861 aaattgcata tttccgccgg gcagcgtggt attccattta tttccggcca gagcaaacat
     1921 tggcagcaac gacgctaaca gaaataatct cttccttttc atcgttattt ttatcgcaca
     1981 agggtgggca tccctgcccg taatgacgtc cttgaacctg ggtaggttat tgatactgaa
     2041 ccttgaaggt tgcatccgca ttagctgcac ccggggttgc ctcgccgatt gcataataac
     2101 gcgcctggaa cggaatggtg ttggtaccgt tattcagggt tgtttgtgca ctaaatgtcg
     2161 caccgtccag cgtcagagca ttgcctgttc tgtctaggat ctgcacgcca acgtttgttg
     2221 cactacctgc agcagaactc tgcagagcca gtacatcagt acgcgtagca tcaattgccg
     2281 tacctaagaa ggcaacagcg gcttttgtgg caacagtggt atcgcaatca ttcagctgaa
     2341 tgttaaaacc aacggcagag ctggttgctc cagcctgctt caggctagcg gtacgaacct
     2401 gtcccaactg aacggtttga tcaacagagc ctgcatcaac tgcgcaagcg gcgttaacaa
     2461 cttccccttt aaagtgaatg gtcccaccat ttaccgtcgt agtatcggcc agagccgctg
     2521 cggaactgag ggacagagcc gacagaacaa cgattgccag agttttaatt ttcatgctgc
     2581 tttcctttaa aaaaactatt tctaaatcga catgggcagt cgttctgtac actttgtttt
     2641 gtcaacgagt ttgactgcca acactgcaca gttttccccc aaaagatgaa acatttgggg
     2701 ccattttgac tcatagagga aagcatcgcg cacaaacttt ttcagtttat ttgttggctt
     2761 aatattctat tattatcttt atttatagat ttttatattg catgaggtgg tttttggaga
     2821 gaagaatgag gaagatgcgt cgagccacag aaacgttagc tttacatata gcggaggtga
     2881 tgtgaaatta atttacaaga gaaataattt acatatcaaa tagttagatg ctttttgtcg
     2941 ttttttaata tttttatgct tgagaaaaaa tacgtaactt atttatgata tggacagttt
     3001 ggccccaatt gtcttgtgtt tatttgtttt ttaactttat tatcaataag ttaaatcaaa
     3061 cctcttctct ttttaatttt tcgtttatga gattatttct ttcccataat ccggcaaaac
     3121 gagcagcatt actggcggta taacgcacag tatggcgaat atttcgatgc ccgagataat
     3181 cctgaattaa acgggtatct gcaccacgct ccgccagttc ataaccgcaa gcatgcctta
     3241 acatatgagg atgagtctgc gttacggttc cagcttcaat accggcatcg cgaataatgc
     3301 gataggcctg ctggcgagaa agccgactcc cgcggcgaga aataaatata gcgtcggtcc
     3361 ggtcagcgcc tttccagtta gcacgttcct gggtccagcg ttccacggct tcacgctcat
     3421 caaaacgtaa cgggtgaacg gtagaaaatc cgttcttcag tcggcgaata tttattcgac
     3481 cttcattaag gtcaaggtcc tgataatgca gatcaagcag ttcactaata cgcatcccat
     3541 gccgatatgc caacagaata agacaataat ctctggctcc cgttgccccg taacaaaccg
     3601 cctgcatcat ggcctgaact tctttaccgg taagataacg acgtttactc acaatagtag
     3661 tactcctaac tgagatatat ttgaatgaat acctatagga aacctcaatc ggtcaaaatt
     3721 agcccaatag ataaaaagat aaccacacac gaagttatgt ggctatcagt caattacatt
     3781 aaacatcata caaaaataaa atatcactga tgtgacaaca tctattttca ttgatttgat
     3841 tcaatataac aatagattac agaggattaa cttccagtta cttaaaaaat aaagaaaaaa
     3901 gtgtggaaac aggagaattt aagtgatgtg taaacttatg aatgattttg tttatttatt
     3961 ttgaagaaca taaatcatta gattttgtat gggtatggtt aacttaatgt ttattatgat
     4021 aattaagaat cacacttaca ataacatcaa ttaataaatg gttaatcatt tttgatatat
     4081 cgtaagaata atgtagtttt taacaccatc cctggtatct caactatctc tataaaacag
     4141 catgacgctg tcgtcctctg gctctatccc agatgccgta aaaacgccct gcattgctgg
     4201 cggtatacca gacagtatga cgaatattgc gatgcccaag ataatcctag ataagtcgcg
     4261 tatctattcc catattcgcc aaagcaaaac cacacgaata gcgtaacatg tgcggatgaa
     4321 tctccagtga caacccggca ttaccaccag aagtcgagat aatatggtaa aactgttgcc
     4381 gagaaagcgg attcccctta cgtgataaaa atacccactc gctctcagca tgcggataag
     4441 aagtacggat actcaaccag ttttttaaag cctgaacttc tttgttcaat agcgggtgcg
     4501 ttgttgaaaa gccttttttt aatcgatgga tatatataca ctttgcttta agatcaatat
     4561 ccgaaatcct caatcgacaa atttcactcg cccggaaacc atgaataaaa caaagcaaaa
     4621 tcagacaata attacgtgtt gcatgaggcc cggtatttgc tgctttaagg agtgactcga
     4681 tttcactatg ggtaaggaag ttcctttttt tgttatcagc cttattcctc atcgttttcc
     4741 cttataatta cagacgcgca ctagctgtgc tgggttagca ataatccaac atttttatcc
     4801 tgattatgtt tataaaagcg aacgtttgct taataactta cgtaagtgga ccagttcttc
     4861 tgagcgaact taaatggagt agcgatagtt acttataata actttgccat acaaacgcca
     4921 tgctcttgca tgctatgtac ctttatatat ttatcaatct gatacgaatc taataataac
     4981 cctcatcatt aatgcaacta atcttatcta tatatcatgt gatatgttct gtaacaagta
     5041 aaatcaacat aaaaaattca gcaaaattta atatttgtta aactagccgt ctcaaatatg
     5101 acattattag ttaactgcat ttgccacaaa atggctattt catggaacgc agtaatttct
     5161 tagtggctat tatcatgcta attcttagat ccctagcgat tattcctgcc tggttcgtca
     5221 gcttaggcca tgagattgtt acattccttt ttatcctgga gatatggggg aacgcaggcg
     5281 acgctttttc atctacatcc gttggtaaat atagctttgg tcactacgac ttaagccttc
     5341 ctcaatcaca gctattcgcc gggcgatacg gcaaacaaac taagtgaaac atcatcctga
     5401 aaacaccaac atcaacaagc ctctccagat cgacatcagg agtgaccagt tacaagccac
     5461 aaaccaagaa acatatttca tgatgagaat tatgctcagc ctgatggcga gtaaaccctt
     5521 attttcacca ctctcactga gttagcttgc aaagctttct ttgtttagat tctaaataaa
     5581 ttatttataa aacgtatagt taaaagaaaa tctttagttt ttgaagacca ccgcaagtgt
     5641 tcgtctggct tcacatggca tcttcttctt agaaaaagat cgacatattt tgtgacgcga
     5701 attgcaaatc tggttttgtt gtatggattg cgtgattttt gatctggtat aacaggtata
     5761 aagatgcacc acgatagtca atgagactgg gcatctcgta atctatggca aacatcactt
     5821 cagttctttc acatcgggtg atgaaaatgc acttcagtct gaaaggaata tgaaaatgag
     5881 atcaacagac actctatttt atgactctgg gtaaaatggg ttgagtaagt gatataactt
     5941 acgaatattc aaatcaatta aaaatcagaa gagattttat actcaggtat ttaatctgga
     6001 tctctgttta tttaaataat gttaaaagag aattttcaca ggagacctta tacaaaaaat
     6061 ataaaataca gttacccgtt gccaaagaca ctataagcct ggcaaaaaaa tattacacaa
     6121 cataaatgct aattgtttat gcgggctttg tattgctttc tgtatcctac aaatgagtga
     6181 aatttatgaa aaaggctaaa atactttctg gcgtattatt actgtgcttt tcgtccccat
     6241 taatttctca ggctgcgaca ctggacgtac gtggtggata tcgtagtgga agccacgcct
     6301 atgagactcg actcaaagtc agtgagggat ggcaaaatgg atggtgggca agcatggaaa
     6361 gtaatacctg gaataccatt catgataata aaaaggaaaa tgccgcactc aatgatgttc
     6421 aggttgaagt taattacgca attaaacttg atgatcaatg gacggtgcgc ccgggaatgt
     6481 taacgcattt tagcagcaac ggcacacgct acggacccta cgtaaaactg tcctgggacg
     6541 cgacaaaaga tcttaatttt ggcattcgct atcgttacga ctggaaagct taccgacaac
     6601 aagacttatc cggtgatatg tctcgtgata acgttcatcg ttgggatgga tatgtcactt
     6661 accatattaa tagtgatttc accttcgcat ggcaaacgac gctatacagc aaacagaatg
     6721 attatcgcta tgcaaaccac aagaaatggg cgacggaaaa tgcctttgtt ctacaatacc
     6781 atatgacgcc cgatattacg ccatacatag aatatgacta ccttgaccgc cagggtgttt
     6841 acaacggcag agataattta tcggaaaaca gttatcgcat tggtgtgtca tttaaactgt
     6901 agtagacagg agacagtcac aatgaataaa actataatgg cgcttgctat catgatggct
     6961 tcatttgccg caaacgcgtc tgtattaccg gaaactcctg tgccatttaa aagtggtacc
     7021 ggagcaattg ataacgacac tgtctacatt ggtttaggta gcgcaggtac agcatggtac
     7081 aagctagata cacaggccaa agataaaaaa tggactgcgt tagctgcatt ccctggtgga
     7141 ccgagagagc aagcaacctc tgcatttatt gatggcaatc tgtatgtgtt tggcggcatt
     7201 ggcaaaaaca gcgagggatt gactcaggta tttaatgacg tacacaaata caaccccaaa
     7261 accaatagct gggttaaatt gatgtcgcac gcgccgatgg gcatggcggg tcatgtgact
     7321 tttgtacaca acggcaaggc ttatgttact ggcggtgtta accagaatat cttcaatggc
     7381 tattttgaag atctcaacga ggctggaaaa gattcaaccg ctatagataa aatcaacgcc
     7441 cactattttg acaaaaaagc agaagattat ttcttcaata agtttctgtt gtcttttgat
     7501 ccctcaacac agcaatggag ttacgctggc gaatcgccct ggtacggaac ggctggtgcg
     7561 gcggttgtga ataaaggtga taaaacctgg cttattaatg gcgaagccaa accaggattg
     7621 cgaacggatg ccgtatttga acttgatttc accggtaata atttaaaatg gaataagctt
     7681 gatcccgtct catcaccaga tggcgtcgct ggcggttttg cggggataag caatgattct
     7741 cttatatttg ctggaggggc cggattcaaa ggttcacgag aaaattacca gaacggtaag
     7801 aactatgcgc atgaaggcct gaaaaaatca tatagcactg atattcatct ttggcataac
     7861 gggaaatggg ataaatcggg tgaattatcg caaggtcggg cctacggagt atcattgccc
     7921 tggaataata gtttattaat tattggcggt gaaactgcag gcggcaaagc ggtgacggat
     7981 tcagttttaa tttctgtgaa ggataataaa gtcacagtac aaaactaacg cttcagggcc
     8041 ccggtaaagg ggccctgcta tcaatttgtc atttgtatta cgcgaaaatt atatgaacgc
     8101 aataatatca cccgattatt actatgttct taccgttgct ggtcagtcta atgccatggc
     8161 gtatggcgaa ggactgccat taccggacag ggaagatgcg cctcattcca gaattaaaca
     8221 attagcgaga tttgcgcata cgcatcccgg aggcccgcca tgtcacttta acgacattat
     8281 tccactgact cactgcccac acgatgttca ggatatgcag ggttatcacc atcctctggc
     8341 aacgaatcat caaacacagt acggcaccgt tggccaggca ctgcatattg cacggaaatt
     8401 actgcccttc attcctgata atgcagggat tcttatcgtt ccgtgttgcc gtggcggatc
     8461 ggcttttacc gcgggcagcg aagggacata ttcagaacgg cacggagcca gccatgatgc
     8521 ttgtcgttgg ggaacggata ctccgctgta ccaggattta gtcagcagaa cgcgagccgc
     8581 actggcaaaa aatccgcaga acaaattcct cggcgtatgc tggatgcaag gcgaatttga
     8641 cttaatgacc agtgactacg cgtcacaccc tcaacacttt aatcatatgg ttgaagcctt
     8701 tcgtagggat ctaaaacaat accattctca gcttaataat attactgacg cgccgtggtt
     8761 ttgcggcgat accacctggt actggaaaga aaatttcccg cattcgtatg aagctattta
     8821 tggcaattat caaaataatg ttttagccaa tattattttc gtcgacttcc agcaacaagg
     8881 tgaaagagga ctgacgaacg cgcctgatga agacccggac gatttaagca cgggatatta
     8941 cggttcagcg taccggtcac cggagaactg gacgacggca ctgcgaagca gtcatttcag
     9001 cacggcagcc cgtcggggga ttatttctga cagatttgta gaagcaattt tgcagttttg
     9061 gcgcgaaagg tgagcgggga gtttattcat gggggatgtc cttgttcgtt ctcagagtag
     9121 acaacaaaaa acaaatgaga tcaaggtaat atctcttcac ttgcgggctc gaacagcagg
     9181 caattgccaa tataataatc tggcacaagg gtgaaaaacg cgcaccgcat aaaccgttgt
     9241 tattgcttac agattagccg gatgccttaa tagacatccg cgccttttcg attatggctc
     9301 ggaaatctac gaacatctac acagtttact ggaacgtttt gggccacagc gttcgcagca
     9361 ccgtcctgat atgccgttct ggcgattata agtcgacgga ttct
//
LOCUS       DAJTQQ010000129         9402 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000129 DAJTQQ010000000
VERSION     DAJTQQ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.129"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            60..212
                     /gene="hokE"
                     /locus_tag="OO309_003574"
     CDS             60..212
                     /gene="hokE"
                     /locus_tag="OO309_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR5848577.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            563..715
                     /gene="hokE"
                     /locus_tag="OO309_003575"
     CDS             563..715
                     /gene="hokE"
                     /locus_tag="OO309_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR5848578.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(837..1457)
                     /gene="entD"
                     /locus_tag="OO309_003576"
     CDS             complement(837..1457)
                     /gene="entD"
                     /locus_tag="OO309_003576"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR5848579.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1632..3872)
                     /gene="fepA"
                     /locus_tag="OO309_003577"
     CDS             complement(1632..3872)
                     /gene="fepA"
                     /locus_tag="OO309_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR5848580.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4115..5317
                     /gene="fes"
                     /locus_tag="OO309_003578"
     CDS             4115..5317
                     /gene="fes"
                     /locus_tag="OO309_003578"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR5848581.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5320..5538
                     /gene="ybdZ"
                     /locus_tag="OO309_003579"
     CDS             5320..5538
                     /gene="ybdZ"
                     /locus_tag="OO309_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR5848582.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5535..9380
                     /gene="entF"
                     /locus_tag="OO309_003580"
     CDS             5535..9380
                     /gene="entF"
                     /locus_tag="OO309_003580"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCR5848583.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDI
                     ISPGAFVKIGPIIRATLNR"
BASE COUNT         2049 a         2474 c         2593 g         2286 t
ORIGIN      
        1 ccactccaaa ccagacaatt tggatggtag tcccttcttc gcatggaggt aatataaaca
       61 tgctgacgaa atatgccctt gtggcagtca tagtgctgtg tttgacggta ctgggattta
      121 cgcttctggc cggagactcg ctgtgtgagt ttacggtgaa ggaacgtaat attgagttca
      181 gggctgttct cgcttacgaa ccgaagaagt agccgttgtg cggggagtaa tccccgcata
      241 tccggttgtc aggtcaggat ggtaaggcac ctgctttaca ctttcgcccg cggtcagtga
      301 tggctgcggg cgatttcgtt ttaagagcaa tgatgagttt tgccgacaga agtccgaaac
      361 aggactcact gtataaataa acagctattt tgctaaggaa gggtaagata acggcgggtg
      421 cctgaagctt tccggtttca ggttaactct gaggtttgga aagatgaagc cccaagagat
      481 atttctatca atcctggggc tgccactccg aacctcaaca actcggatgg tagtcccttc
      541 ttcgcatgga ggcaatataa acatgctgac gaaatatgcc cttgtggcag tcatagtgct
      601 gtgtttgacg gtgctgggat ttacgcttct ggtcggagac tcgctgtgtg agttcaccgt
      661 aaaggaacgt aatattgagt ttaaggctgt tcttgcttac gaaccgaaga agtagccgtt
      721 gtgcggggag taatccccgc atatccggtt gtcaggtcag agtggtaagg cacctgcttt
      781 acactttcgc ccgcagtcag tgatggctgc gggcgaatcg taccagatgt tgtcaattaa
      841 tcgtgttggc acagagttat gactattttt tcttttatct gccagtgcac agcaaacatc
      901 tcattctcac gatgaatgat gacctgctgt ttattccagc taattatctg atagtccaga
      961 aaacctgcat cagtttggat ctcacttgcc ttaaatgcgc tctctttggc ggaaaatgca
     1021 agtgtcagcg ccagagaaaa ggctaaaccg cagtctgcga gtcgctcgtg ttctgctggt
     1081 gtaataatgt tgtctgtcaa ttcggtcgct gtttgcgcag aaaaaatttc ttcgatatca
     1141 acgccaatcg gttgacggga aaccacggcc aatgccgtag ccccgcagtg gctaatactg
     1201 ccgtatacct ccgcaggcca caccggttgg cgtagctcgc cgattgcggg cacacattta
     1261 tagccatatt cccgcaaggc ataaacagca gcgatccgtc cggctaaatg ctctgtttta
     1321 cgtttacgtc cagcgtgttg cagttgtgcg tagtgcggca gccagagtaa atcctgctca
     1381 caaaaattcg ccggatcgaa ctcaacaaaa tgcagcgtat gtccggcaaa ggggagggag
     1441 gtatgcgtag ttttcatatc gaccatattc gagactgatg acaaacgcaa aactgcctga
     1501 tgcgctacgc ttatcaggcc tacgtgatcc tgcaatatat tgaattggca agatctttgt
     1561 aggccggata aggcgttcac gccgcatccg gcgtgaacga cgcgcacttt gtcaacaatc
     1621 tgacgttagc atcagaagtg agtattaatg ctcatatacc acgtacgtcc cggctcgtta
     1681 taggtatacg cgccggcacc ggcgatatag ttggctcctg ccagatcgcc cgtggtctgg
     1741 gcattacctg cacgccacaa acgtttgtcg aacaggttgt ccacgccgcc ggtcagactg
     1801 acattcttcg tcacatccca ggtcgcgctc agacccacaa tgctgtaagg actgatttct
     1861 ttggtttccg ggccaaccgc tggctgacct ttatagttgt acttcttcgg ctgctgcttg
     1921 ccgtaccagg tgaaggtcgt ttgcatcgac aaatcttccc gtgcctgcca gctcagcgtt
     1981 gagttcaacg tgtactccgg gatgatcgac aaacggtcgc ccgtggtttt gttttcactc
     2041 ttcagcatat aagtgatgtt attggtccac atcaccgttt cgctaactgg tacgtttaac
     2101 gatccttcca gaccttcaac cactgctttc ggcacgttat cccactgata gagatcagtg
     2161 ccgactgcgt tttgccctac agccacatag cccgcttcaa tcttattgcg ataatcgtta
     2221 cggaaccagg tgacgcccgc cagccagccg tcgcgtttga actccagacc aatctctttg
     2281 ttgatgctgg tttctacttt caggtcatcg ttaccttgca gatagcagcc gcccgcgctg
     2341 gcatagcagc cctgaccttt actgtagaga atgtagttcg ggttagtctg gtataggctc
     2401 ggcgctttat aggcgcgggc aatgcctatt ttcagcgtga agtcatcgcc taaaccttgc
     2461 gatatgttca gcgccgggct ccagttattg ccgacaatac tgtgatgatc gaaacgcagc
     2521 cccggcgtta cgatggtgct gtcagtcagc tccatgttgt tttcggcaaa cagcgagaaa
     2581 atttctgctt ttgaatacgg gctacggtcg gtggcactca cgccatcaat agcgccaccg
     2641 gtattcgttc cggtcagtgc ttgggtgttg gaacttaagt ccttcatccg ttgctgattc
     2701 cactccgtac ccagcgtcag cgtctggtta acgaggaaat caatcggcag gttaacttcg
     2761 ctgtgcagca tcacgtcatc gagatcgata tcgacgaaat cctgtgtcgc tttttcgtta
     2821 aatttccctt cggtaccgcc cgccagacct tccggaatac gcgagttacg ggtgtgttca
     2881 tactgcaccc agttgctggt ggtcacgccg ttatcccagc caccgttcca ggtcagcgcg
     2941 tagttctggc gatacaggcg gttggtttca tcgccatatt tcgagcgagt gtaagcgtca
     3001 gagttggtgt tctgggtatc gcccgcatac aggttaccct ggcggctgta acccgcttcc
     3061 agttccagcg attgcaatgg tgcgaagtcc cagcgcacca cgccgttaat atctttattg
     3121 atcacccctt cgcgcccggc tggtaacgtc gtggcatacg ttccggcacg cgcggactga
     3181 tggccctggt tgatatccca cgcgtcagcc tgagttttgt cgaggttgcc atacaagcgg
     3241 aagctgaatt cgtcgcccag cggaccggtc aggctaaagt tagtgcgttt ggtggcacct
     3301 tcctctttat gttctggcgc attgaaatat gcgtcccagg agccgtgcca ctcgccgctg
     3361 ccttttttgg taatgatgtt aaccacgccg cccgccgcgc cgttgccata acgcgcagct
     3421 gccggaccac gcagaacttc aatacgttca atcatttcag gtggcaccca ggaggtatca
     3481 ccacgggtat cgcgctcgcc acgccagccc tgacgcaccg agttacggct gcttaccggc
     3541 ttgccgtcaa tcaaaatcag cgtgttttcc ggacccatac cgcgaatatc aatctgtcgg
     3601 ttattcccac gctgaccact ggtggagtta ccggtcaggt taacgcctgg catggtacgg
     3661 atgatctccg acacatcgcg ggcaaccggg tttttgcgga tttcatctgc ggtgatggtc
     3721 gaaacgccag gagcctgcaa gttctgctcg gcggcggtaa cgacaatggt atcgtcatgt
     3781 gaaacaggtg tatcggtcgg ctcttgtgcc tgcgctaccc cataaatccc cagattgacc
     3841 aacaaggcca gggaatgaat cttcttgttc attgttttat tcctgcattt ttgccacgaa
     3901 ttgcaactgt cgggcatggt cgtcatcaac acgacgcatc ccgttaccgc gaaaaccttt
     3961 gatcctgaaa gacacgcagt gcagttggtt aattaatgtc cgcgcttccc acggcgcgcc
     4021 attacgctat tgcaaatgca aatagttatt aataacatta tcaatatatt tctgcaatca
     4081 atgaaaaatt gcacagtaaa catggggtta tggtgtgacg gcgttaaaag taggaagtga
     4141 gagctggtgg cagtcgaaac atggcccgga atggcagcgt ctgaatgacg aaatgtttga
     4201 ggtcactttc tggtggcgtg atccccaagg ttctgaagaa tactcgacga taaagcgcgt
     4261 atgggtctac atcactggtg tgaccgatca tcatcagaac agccagcccc agtcgatgca
     4321 gcgaattgca ggtactgatg tctggcagtg gacgacacaa ctcaatgcca actggcgcgg
     4381 cagctactgc tttattccca ccgaacgcga tgacattttt tctgcaccat cccccgatcg
     4441 cctcgaatta cgtgaaggct ggcgaaaact attaccccag gcaatagccg atccgctgaa
     4501 tccacaaagc tggaaaggcg ggctagggca cgctgtttct gcactcgaaa tgccgcaagc
     4561 gcctctgcaa ccgggatggg attgtccgca agcgccagaa ataccagcca aagaaattat
     4621 ctggaaaagt gaacggttga aaaattcacg tcgtgtatgg atttttacca ccggcgatgt
     4681 aacagcagaa gaacgcccgc tggcagtttt gctcgatggc gaattttggg cgcaaagtat
     4741 gcccgtctgg cctgtgctga cttcgctgac ccatcgtcag caacttcctc ccgccgtgta
     4801 tgtgttgatc gacgctatcg acaccacgca ccgcgcccac gaactgccgt gtaatgcgga
     4861 tttctggctg gcagtacagc aagagttatt acccctggtg aaagttattg ccccttttag
     4921 cgatcgcgcc gatcgcaccg tggtcgccgg gcagagtttt ggtgggctgt ccgcgctgta
     4981 tgccggactg cactggcctg aacgctttgg ctgtgtatta agccagtcag gatcgtactg
     5041 gtggccgcat cggggcgggc agcaagaggg cgtgttactt gaaaagctaa aagctggtga
     5101 agttagcgcc gaaggtctgc gcattgtgct ggaagcgggt attcgcgagc cgatgatcat
     5161 gcgggccaat caggcgctgt atgcgcaatt acaccctata aaagaatcca ttttctggcg
     5221 tcaggttgac ggcggacatg atgcgctttg ttggcgcggt ggcttgatgc aggggttaat
     5281 cgacctctgg caaccacttt tccatgacag gagttgaata tggcattcag taatcccttc
     5341 gatgatccac agggagcgtt ttacattttg cgcaatgcgc aggggcaatt cagtctgtgg
     5401 ccgcaacaat gcgccttacc ggcaggctgg gacattgtgt gtcagccgca gtcacaagag
     5461 tcctgccagc agtggcttga agcccactgg cgtactctga caccggcgaa tttcacccag
     5521 ttgcaggagg cacaatgagc cagcatttac ctttggtcgc cgcacagccc ggcatctgga
     5581 tggcagaaaa actgtcggat ttaccctccg cctggagcgt ggcgcattac gttgagttaa
     5641 ccggagaggt tgatgcgcca ttactggccc gcgcggtggt tgccggatta gcgcaagcag
     5701 atactttgcg gatgcgtttt acggaagata acggcgaagt ctggcaatgg gtcgatgatg
     5761 cgctgatatt cgaactgcca gaaattatcg acctgcgaac caatattgat ccgcacggta
     5821 ctgcgcaggc attaatgctg gcggatttgc aacaagattt gcgcgtcgat agcggtaaac
     5881 cactggtctt tcatcaactg atacaggtgg cagataaccg ctggtactgg tatcagcgtt
     5941 atcaccattt actggtcgat ggcttcagtt ttccggccat tacccgccag atcgccaaca
     6001 tttactgcgc attgctgcgt ggcgaaccaa cgcctgcttc gccgtttacg cctttcgctg
     6061 atgtagtgga agagtaccag caataccgcg aaagcgaagc ctggcagcgt gatgcggcat
     6121 tctgggtgga acagcgtcgc caactgccac cgcccgcgtc actttctccg gcacctttag
     6181 cggggcgcag cgcctcggca gatattttgc gtctgaaaat ggaatttacc gacggggaat
     6241 tccgccagct ggctacgcaa ctttcaggtg tgcagcgtac cgatttagcc cttgcgctga
     6301 cggctttgtg gctggggcga ttgtgcaatc gcatggacta cgccgccgga tttatcttta
     6361 tgcgtcgact gggctcggcg gcgctgacgg ctaccggacc cgtgctcaac gttttgccgc
     6421 tgggtattca tattgcggcg caagaaacgc tgccggaact ggcaacccga ctggcaaccc
     6481 gactggcagc acaactgaaa aaaatgcgtc gtcatcaacg ttacgatgcc gaacaaattg
     6541 tccgtgacag cgggcgagcg gcaggtgatg aaccgctgtt tggtccggta ctcaatatta
     6601 aggtatttga ttaccaactg gatattcctg gtgttcaggc gcaaacccat accctggcaa
     6661 ccggtccggt taatgacctt gaactggccc tgttcccgga tgaacacggt gatttgagta
     6721 ttgagatcct cgccaataaa cagcgttacg atgagccaac gttaatccag catgctgaac
     6781 gcctgaaaat gctgattgcc cagttcgccg cggatccggc gctgttgtgc ggcgatgtcg
     6841 atattatgct gccaggtgag tatgcgcagc tggcgcagat caacgccact caggttgaga
     6901 ttccagaaac cacgctgagc gcgctggtgg cagaacaagc ggcaaaaaca ccggatgctc
     6961 cggcgctggc agatgcgcgt tacctgttca gctatcggga aatgcacgag caggtggtgg
     7021 cgctggcgaa tctgctgcgt gagcgcggcg ttaaaccagg ggacagcgtg gcggtggcac
     7081 taccgcgctc ggtctttttg accctggcac tccatgcaat agttgaagct ggcgcggcct
     7141 ggctaccgct ggataccggc tatccggacg atcgcctgaa aatgatgctg gaagatgcgc
     7201 gtccgtcgct gttaattacc accgacgatc aactgccgcg ctttagcgat gttcccaatt
     7261 taaccaacct ttgctataac gccccgctta ccccccaggg cagtgcgccg ctgcaacttt
     7321 cacaaccgca ccacacggct tatatcatct ttacctctgg ctccaccggc aggccgaaag
     7381 gggtaatggt cgggcagacg gctatcgtca accgcctgtt gtggattcaa aatcattatc
     7441 cgctcacagg cgaagatgtc gttgcccaaa aaacgccgtg cagttttgat gtctcggtgt
     7501 gggagttttt ctggccgttt attgccgggg ctaaactggt gatggctgaa ccggaagcgc
     7561 accgcgaccc gctcgctatg cagcaattct ttgccgaata tggcgtaacg accacgcact
     7621 ttgtgccgtc gatgctggca gcatttgttg cctcgctgac tccgcaaacc gctcgccaga
     7681 gttgcgtgac gttgaaacag gttttctgta gtggtgaggc cttaccggct gatttatgcc
     7741 gcgaatggca acagttaacc ggcgcgccgt tgcataacct gtatggcccg acggaagcgg
     7801 cggtagatgt cagctggtat ccggcttttg gcgaggaact ggcacaggtg cgcggcagca
     7861 gtgtgccgat tggttatccg gtatggaata cgggtctgcg tattcttgat gcgatgatgc
     7921 atccggtgcc gccgggtgtg gcgggtgatc tctatctcac cggtattcaa ctggcgcagg
     7981 ggtatcttgg acgacccgat ctgaccgcca gccgctttat tgccgatcct tttgctcctg
     8041 gtgaacggat gtatcgtacc ggagacgttg cccgctggct ggataacggc gcggtggagt
     8101 acctcgggcg cagtgacgat cagctaaaaa ttcgcgggca gcgtatcgaa ctgggcgaaa
     8161 tcgatcgcgt gatgcaggcg ctgccggatg tcaaacaagc cgttacccac gcctgtgtga
     8221 ttaaccaggc ggcagccact ggtggtgatg cgcgtcagtt ggtgggctat ctggtatcgc
     8281 aatcgggcct gccgttggat accagcgcat tgcaggcgca gcttcgcgaa acattgccgc
     8341 cgcatatggt gccagtcgtt ctgctgcaac tgccgcagtt gccacttagc gccaacggca
     8401 agctggatcg caaagcctta ccgttgcctg aactgaaggc acaagcgcca gggcgtgcgc
     8461 cgaaagcggg cagtgaaacg attatcgccg cggcattcgc gtcgttgctg ggttgtgacg
     8521 tgcaggatgc cgatgctgat ttctttgcgc tgggcggtca ttcgctactg gcaatgaaac
     8581 tggcagcgca gttaagtcga cagtttgctc gtcaggtgac gccggggcag gtgatggtcg
     8641 cgtcaaccgt cgccaaactg gcaacgatta ttgatggtga agaggacagc tcccggcgca
     8701 tgggattcga aaccattctg ccgttgcgtg aaggtaatgg cccgacgctg ttttgtttcc
     8761 atccggcgtc cggttttgcc tggcagttta gcgtgctctc gcgttatctc gatccacaat
     8821 ggtcgattat tggcattcag tcgccgcgcc ctcatggacc catgcagaca gcgacaaacc
     8881 tggatgaagt ctgcgaagcg catctggcaa cgctacttga gcaacaaccg cacggcattg
     8941 cggcgcggct gcgtgcccgt ggcgaacagg tggcatttct tggtttgctg gatacctggc
     9001 cgccagagac gcaaaactgg caggaaaaag aggctaatgg tctggacccg gaagtgctgg
     9061 cggagattaa ccgcgaacgc gaggccttcc tggcggcaca gcagggaagt acttcaacgg
     9121 agttgtttac caccattgaa ggcaactacg ctgatgctgt gcgcctgctg acgactgctc
     9181 atagcgtacc gtttgatggt aaagcgacgc tgtttgttgc tgaacgcacg cttcaggaag
     9241 gtatgagccc cgaacgcgcc tggtcgccgt ggattgcgga gctggatatc tatcgtcagg
     9301 attgtgcgca tgtggatatt atctctccag gggcgtttgt aaaaattggg ccgattattc
     9361 gcgcaacgct aaacaggtaa attaatatta tctataaacc ca
//
LOCUS       DAJTQQ010000130         9375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000130 DAJTQQ010000000
VERSION     DAJTQQ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.130"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(26..214)
                     /locus_tag="OO309_003581"
     CDS             complement(26..214)
                     /locus_tag="OO309_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5848584.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMVMTPTY"
     gene            complement(433..1410)
                     /gene="epmA"
                     /locus_tag="OO309_003582"
     CDS             complement(433..1410)
                     /gene="epmA"
                     /locus_tag="OO309_003582"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR5848585.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1735..3543
                     /gene="frdA"
                     /locus_tag="OO309_003583"
     CDS             1735..3543
                     /gene="frdA"
                     /locus_tag="OO309_003583"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR5848586.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3536..4270
                     /gene="frdB"
                     /locus_tag="OO309_003584"
     CDS             3536..4270
                     /gene="frdB"
                     /locus_tag="OO309_003584"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5848587.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4281..4676
                     /gene="frdC"
                     /locus_tag="OO309_003585"
     CDS             4281..4676
                     /gene="frdC"
                     /locus_tag="OO309_003585"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5848588.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4687..5046
                     /gene="frdD"
                     /locus_tag="OO309_003586"
     CDS             4687..5046
                     /gene="frdD"
                     /locus_tag="OO309_003586"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5848589.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5109..6241
                     /locus_tag="OO309_003587"
                     /pseudo
     CDS             5109..6241
                     /locus_tag="OO309_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
     gene            6330..6863
                     /gene="blc"
                     /locus_tag="OO309_003588"
     CDS             6330..6863
                     /gene="blc"
                     /locus_tag="OO309_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5848590.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6860..7177)
                     /gene="sugE"
                     /locus_tag="OO309_003589"
     CDS             complement(6860..7177)
                     /gene="sugE"
                     /locus_tag="OO309_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5848591.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7353..7499)
                     /gene="ecnB"
                     /locus_tag="OO309_003590"
     CDS             complement(7353..7499)
                     /gene="ecnB"
                     /locus_tag="OO309_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5848592.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7610..7735)
                     /gene="ecnA"
                     /locus_tag="OO309_003591"
     CDS             complement(7610..7735)
                     /gene="ecnA"
                     /locus_tag="OO309_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5848593.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7787..8353)
                     /gene="efp"
                     /locus_tag="OO309_003592"
     CDS             complement(7787..8353)
                     /gene="efp"
                     /locus_tag="OO309_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5848594.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8395..>9375
                     /gene="epmB"
                     /locus_tag="OO309_003593"
     CDS             8395..>9375
                     /gene="epmB"
                     /locus_tag="OO309_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR5848595.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIG"
BASE COUNT         2369 a         2410 c         2407 g         2189 t
ORIGIN      
        1 ggcattatct gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg
       61 gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt
      121 aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc
      181 ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg
      241 tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat
      301 acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa
      361 aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt
      421 aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc
      481 gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat
      541 accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc
      601 cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc
      661 atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg
      721 atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa
      781 ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa
      841 cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc
      901 ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg
      961 ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac
     1021 ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc
     1081 agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg
     1141 cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag
     1201 attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat
     1261 atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc
     1321 aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc
     1381 gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac
     1441 gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg
     1501 cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat
     1561 aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca
     1621 gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca
     1681 ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa
     1741 acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt
     1801 gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg
     1861 cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc
     1921 ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc
     1981 gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc
     2041 ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc
     2101 gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag
     2161 acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg
     2221 gttgatgatg gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg
     2281 cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac
     2341 aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt
     2401 ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc
     2461 ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt
     2521 tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat
     2581 atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc
     2641 aacaccatct ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag
     2701 aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc
     2761 gatccggtta aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc
     2821 gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc
     2881 tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc
     2941 ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac
     3001 gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac
     3061 caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa
     3121 ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag
     3181 ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac
     3241 ctgctctaca ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc
     3301 gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag
     3361 cgtgacgacg tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact
     3421 cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt
     3481 ggcgaagcgg atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc
     3541 tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc
     3601 gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg
     3661 ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat
     3721 ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct
     3781 gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga
     3841 tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat
     3901 cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa
     3961 gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt
     4021 tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga
     4081 agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt
     4141 atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc
     4201 tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa
     4261 accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt
     4321 ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg
     4381 ttccggctgt gtggttcagc attgaactga ttttcgcgct gtttgccctg aaaaatggcc
     4441 cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc
     4501 tgatcactct gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag
     4561 cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct
     4621 gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag
     4681 cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc
     4741 ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt
     4801 ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc
     4861 gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt
     4921 ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa
     4981 tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca
     5041 atctaacgca tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac
     5101 cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat
     5161 ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc
     5221 aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta
     5281 cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt
     5341 taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg
     5401 aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga
     5461 atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc
     5521 cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat
     5581 gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg
     5641 ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac
     5701 tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg
     5761 gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg
     5821 gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta ccccccttga
     5881 tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct actggcaaac
     5941 cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca atcctgacag
     6001 catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa aagcgattaa
     6061 gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga ccggcggatt
     6121 tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc tggctaacaa
     6181 aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg ctctacagta
     6241 aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct gtcatctaca
     6301 cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt gccgcagcga
     6361 cagctgcatt tctggtcgtt gcctgtagtt ttcctacgcc gccgcgtggc gtgaccgtag
     6421 tgaataattt cgacgccaaa cgttatctcg gtacctggta tgagattgcc cgttttgatc
     6481 accgttttga acgtggactg gaaaaagtca ccgcaacata cagcctgcgt gatgacggcg
     6541 gcctgaatgt cattaataaa ggctataacc ctgacagagg aatgtggcag cagagtgaag
     6601 ggaaagcgta ctttaccggc gcaccaactc gcgctgcgct gaaagtgtca ttctttggtc
     6661 ctttctatgg cggttataac gttattgcac tcgatcggga ataccgccat gcgctggttt
     6721 gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt tctgacgaag
     6781 tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt aagtttattt
     6841 gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc cccaatacga
     6901 ttaacgccag actcgccaga cgcatcgggt tagcggactc accgagcagc acaatgccgg
     6961 tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc cctaccggta
     7021 acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc acggtaataa
     7081 cactcggcgt caaacggcta aagccgtggg tatatttcag gccaacggcc cataccactt
     7141 ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga aatttggggc
     7201 cgtccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt tgaacggata
     7261 ttttgccagg tggcagggga atttcaacta ttacctttat gacgatcgca gaaaaaacaa
     7321 acggcacgac actgctgtcg taccgttatt gcttattgct gcgctttcgt tgctgcgcca
     7381 gaaatcgcgt taccgccatc agaaatgtct tcaccaacgc cacgcgtggt gttgcaggca
     7441 gttaatactg ttgaaagcac cagaacagaa aagatcgctg caattgtctt cttcaccata
     7501 acgtcttcct tttaggtaat gttatttatg tttgcctata gcaaattaat aatagacaac
     7561 atcgccaaaa atgatggaaa tctgatgatt tttcggaaga gaagaaaaat tagctggcag
     7621 cgcgagagat ggagttaccg agatgtttga tgtcttcgcc gaaaccgcaa gcggtgttac
     7681 agcccgtgag cagcgtgctg gcaagcaaaa caagaacgat aaggcgtttc atcatctctg
     7741 acctgaataa gtgatggtgc agcctgcagg ccgcaccaca accgcattac ttcacgcgag
     7801 agacgtattc accagagcgg gtatccactt tgatgacttc gccgatttgt acgaacagcg
     7861 gaactttaac cacagcgcca gtagacaggg tagccggttt gccgccagta cctgcggtat
     7921 cacctttcag gcccggatcg gtatcaacga tttccagttc aacgaagttc ggcggagtaa
     7981 cggagatcgg ctgaccattc cacagagtta cgatacactc tgcctgatcc agcagccatt
     8041 tagcattgtc gccaattgct tttgcatcag cagacagctg ctcgaaagtt tcgttgttca
     8101 tgaagtgcca gaactcaccg tcgttgtaca ggtaagtcag gttcatatcg acaacatcag
     8161 cgccttcagc ggaatcagta gatttgaagg ttttttctac gcgagtaccg gtcagcagac
     8221 gacgcagttt aacgcgagca aatgcctggc ctttacccgg ttttacgaat tcactcgctt
     8281 caaccgcgta aggttcgccg tctaacatga ttttaagacc agcacgaaaa tcgttgctat
     8341 agtacgttgc cataaggccc tctgaaattt gttaattggt agctaagcca caaaatggcg
     8401 catattgtaa ccctaaatac cccatccaga gaagattggt taacgcaact tgccgatgtt
     8461 gtgaccgatc ctgatgaact tctgcgtctt ttgaatatag acgcggacga aaaactgttg
     8521 gccggacgca gcgccaaaaa gctgtttgcc ctgcgtgtgc cccgctcatt tatcgatcgc
     8581 atggagaaag gcaatccgga cgatcctctt ttgcgtcagg tacttacctc gcaagatgag
     8641 tttgtcgtcg cgtccggatt ctccaccgac ccactggaag aacagcacag cgtagtgcct
     8701 ggtttgttgc ataaatacca caaccgggcg cttttgctgg tcaaaggcgg ctgcgcggta
     8761 aattgccgct attgcttccg tcgccacttc ccctatgccg aaaatcaggg caacaagcgt
     8821 aactggcaga ctgcacttga gtatgttgct gcgcatccgg aactggacga gatgattttc
     8881 tccggcggcg atccgctgat ggcgaaagag cacgagctgg actggttgct cacacaactg
     8941 gaagccatcc cgcatatcaa acgtctgcgg attcacagcc gtctgccgat tgtgatcccg
     9001 gcacgtatca ccgaggcgct ggttgaacgc tttgcccgtt ctacgctgca aatcttgctg
     9061 gtgaatcaca tcaatcatgc caatgaggta gatgaaacgt tccgtcaggc gatggctaaa
     9121 ttgcgccgtg tcggtgtcac cctgcttaac cagagcgttc tgttacgtgg tgtgaacgat
     9181 aacgcacaaa cgttggcaaa cctgagtaat gcgttgttcg atgccggcgt gatgccgtat
     9241 tacctgcatg tgctagataa agtacagggc gcggcgcatt ttatggtgag tgatgacgaa
     9301 gcacggcaga ttatgcgtga gttgctgaca ttggtgtcgg gatatctggt gccgaaactg
     9361 gcgcgagaaa ttggc
//
LOCUS       DAJTQQ010000131         9291 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000131 DAJTQQ010000000
VERSION     DAJTQQ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.131"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            18..869
                     /gene="hchA"
                     /locus_tag="OO309_003594"
     CDS             18..869
                     /gene="hchA"
                     /locus_tag="OO309_003594"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR5848596.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(977..2335)
                     /gene="hprS"
                     /locus_tag="OO309_003595"
     CDS             complement(977..2335)
                     /gene="hprS"
                     /locus_tag="OO309_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR5848597.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2335..3006)
                     /gene="hprR"
                     /locus_tag="OO309_003596"
     CDS             complement(2335..3006)
                     /gene="hprR"
                     /locus_tag="OO309_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR5848598.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3139..3552
                     /gene="hiuH"
                     /locus_tag="OO309_003597"
     CDS             3139..3552
                     /gene="hiuH"
                     /locus_tag="OO309_003597"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR5848599.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3661..4665
                     /gene="msrP"
                     /locus_tag="OO309_003598"
                     /pseudo
     CDS             3661..4665
                     /gene="msrP"
                     /locus_tag="OO309_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4666..5301
                     /gene="msrQ"
                     /locus_tag="OO309_003599"
     CDS             4666..5301
                     /gene="msrQ"
                     /locus_tag="OO309_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR5848600.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            5559..6209
                     /gene="zinT"
                     /locus_tag="OO309_003600"
     CDS             5559..6209
                     /gene="zinT"
                     /locus_tag="OO309_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR5848601.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(7287..7556)
                     /locus_tag="OO309_003601"
     CDS             complement(7287..7556)
                     /locus_tag="OO309_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5848602.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            7613..8281
                     /locus_tag="OO309_003602"
     CDS             7613..8281
                     /locus_tag="OO309_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5848603.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            8597..>9291
                     /locus_tag="OO309_003603"
     CDS             8597..>9291
                     /locus_tag="OO309_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5848604.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT         2668 a         2092 c         1897 g         2634 t
ORIGIN      
        1 acaataagga atacactatg actgttcaaa caagtaaaaa cccgcaggtc gatattgctg
       61 aagataatgc gttcttccct tcagaatatt cgcttagcca atatacgagt cccgtctctg
      121 atcttgatgg cgtggactat ccaaaaccgt atcgcggtaa acataaaatt ctggtgatcg
      181 ccgcggacga acgttatttg ccgaccgata acggaaaact gttctcgacc ggtaaccatc
      241 cgattgaaac gttgctgccg ttgtatcatc tccatgctgc aggtttcgaa ttcgaagtgg
      301 cgaccatttc cggtctgatg accaagtttg aatactgggc tatgccgcac aaagatgaaa
      361 aagtaatgcc attctttgaa cagcataaat cgttattccg taatccgaag aaactcgcgg
      421 atgttgtcgc cagcctcaac gctgatagcg aatatgcagc aatctttgtt cctggtgggc
      481 atggcgcact tattggttta ccagaaagcc aggacgtggc tgccgcttta cagtgggcaa
      541 tcaaaaatga ccgttttgtt atctcccttt gccacggtcc ggcggctttt ctggcacttc
      601 gccacagcga taacccactg aatggttatt ctatttgcgc attcccagac gccgcagaca
      661 aacaaacgcc agatattggc tatatgccgg gtcatctcac ctggtacttc ggcgaagagt
      721 tgaagaaaat gggcatgaac atcattaatg acgacatcac cgggcgagta cataaggacc
      781 gtaaacgtct caccggcgac agtccttttg cagcgaatgc gttgggtaaa ctggcggcgc
      841 aggaaatgct ggcagcttac gcgagttaat ccctgttaca ttgtgatgta agcgcataat
      901 caatcgcagc cacgttaata ctcacgacgt ggctgcgttt tttcacggtt aacttatggc
      961 gtactgaagc ccgatgttaa tttctttgcg gtaacgtaat tcggaacacg ttatgcttac
     1021 tgagatagtg atacgtagca cttcctccat gtaattcggc aatcgctttg actaaagaaa
     1081 ggccaagtcc ctgacctacg gaatgacgcg aattatctcc ccgccaaaat ctacggaaga
     1141 gtttttcagg ctcattaatt ttcgttccag gactggcgat atcaatatta agagagccgt
     1201 tggcatcaag aaaactggtt atatgaatac gcgatttttc tggtgaatat cgaatggcgt
     1261 taacaataag attcgataac attcgttgca gtaaaatttt atccgcaaag atttgttgat
     1321 tgcacttgac cttaaagcaa atctctttct cgtctgaaag atattcaaga tagtccaaaa
     1381 gattttcaac ttccttattg agagaaagcg aatccagttt caccaaaaca ttgtttttgt
     1441 ctgccctggc aagaaacagt atattctctg ttaaccgcga aatattttcc agctcttcaa
     1501 tgtttccggc aattgttttt tgatattcag cgatacttct gatttgactg agcgtaacct
     1561 gattctgacc cagtaatgca ttaattggcg ttctaagttc atgagcgaga tcgtcagcaa
     1621 attgactaag gcgctcaaaa tctttgacta aagcatgatg cattttattc aacgcctgcc
     1681 caagaggttt tagttctctc ggtaatgcgt taacctcaac cggcactcgg ctatcgttat
     1741 aattcagcgc ttccgttacg ccactcaact ttttgatctc tcgcaacccc gtcctgatta
     1801 acaacggact taatactgag cacagtataa tggcgacaat gcaaattata atgctattaa
     1861 ttttatactg ttcaagcata ttatgtctgg ctgaagccaa tttagccaca gtaaccgtta
     1921 atgatggcga aacttcatca atgttaattc gtaaagcatc tatctctgta tcattaataa
     1981 tgcttctgta aatgcctgct gcgctgattg tctcactagc aggtatgtta tttaacatgt
     2041 catcactgac atttgtccgg ttaacaattt tattgatgcc atcactatga atgatcaaaa
     2101 tatcctgact aacatccatc atccggttaa agtacacagg taacgtatct ggatttaccc
     2161 catcaattaa caactgcttg atctgcgctg tccggttaat gagtgttgta tcatcgcgcc
     2221 atttcaactc acttgccagg ccattataga gagtccatac aattccagcg ccagcaacag
     2281 actgcagaaa tataaaaagc aaggttaaac ggacggttat agataatctt ttcattattt
     2341 ttttaccgct acgaatgaat agcccatccc ccggattgtg gcgattagct tttcaggaaa
     2401 aggatcatca acttttgcgc ggagcctgcg aatggcgaca tccaccgtat tggtatcact
     2461 atcaaagttg attccccaaa tttcactcgc aataaccgtt ctgggtataa tctcgccagc
     2521 tctggaggcc agtagccaaa gtaactgaaa ctccttgcgc gtcagtgtaa tactgatatt
     2581 gtccctgctg acactttgac tgacagagtc catttttaag ccactgattt ctaatgttga
     2641 attcagagcg tgatgttgcc ttaattgtgc ccgaaccctt gctagcaact cagaaaatga
     2701 aaaaggtttt accagataat catttgcccc actgtccaac cctctgactc tgtcatcgac
     2761 agaatccctt gcagtaaggc aaataacagg ggtttgcttt gccgttctta acgtttgtaa
     2821 gatctgccag ccatccatac ccggaagcat aatatccaga atgatcaatg cataatcatc
     2881 cttcagcgca agataaagcc catctctgcc atcagaaacg gcatcaatga cataacccgc
     2941 ttcggaaagc ccctgcgtta cccattcctg ggtcctttga ttatcttcaa taagtagaat
     3001 cttcatgaaa ataatatgcc ataaatcata tgttatagaa acaacctggt tcattacaaa
     3061 attgtaatgc tgctgtaagg ttaccctgga cgttttttcg ctatcctcaa aactcattca
     3121 catgacaagg atataaacat gttaaagcgt tatttagtac tctcggtagc aacggcagcc
     3181 ttttcattac cttctttggt ttatgccgca caacaaaaca ttcttagcgt gcacattttg
     3241 aaccagcaaa caggaaaacc cgctgccgac gtgacagtca ctcttgaaaa gaaagcggat
     3301 aacggctggt tacaacttaa taccgccaaa acagataagg atggacgaat taaggcactg
     3361 tggcccgagc aaactgcgac tactggcgat taccgtgtcg tatttaaaac cggggactat
     3421 ttcaagaaac aaaatcttga aagtttcttc cctgagattc ccgttgaatt tcatattaat
     3481 aaagtgaacg agcattatca tgtgcctttg ttactcagcc aatatgggta ttcaacctat
     3541 cgtggcagtt aatgcgtaaa tatgtaaaag ctgatctctg ctactgttag ggaggtgact
     3601 ccctgacaga ttaacagtaa acagctcttg ttggttaacg acaaaaaagt gtgatggctt
     3661 atgaaaaaga agcaattttt aaaagaatca gatgttacgg ccgagtcggt attctttatg
     3721 aagcgtcggc aggtgttaaa agcactgggc atcagcgcag ctgcattttc tttgcctcac
     3781 gctgcgcatg ccgatctgct tagctggttt aaagggaacg atcgtccgcc cgcccccgcc
     3841 ggaaaaccgc tggagttcag caagcctgcc gcctagcaaa ataacctgcc actgacgcca
     3901 gcagataaag tctccggtta taacaacttc tatgaattcg ggctggataa agacgatccc
     3961 gcggctaatg ctggtagcct gaaaaccgat ccatggacac tgaaaatcag cggcgaagtg
     4021 gcaaaaccat tgaccctcga tcatgatgat ttaacccgtc gcttcccgct ggaagagcgt
     4081 atttatcgta tgcgctgcgt ggaagcgtgg tcgatggtgg tgccgtggat tggttttccg
     4141 ctacacaaat tgctggcgct tgccgaaccc accagcaatg cgaagtatgt cgctttcgaa
     4201 acgatttatg cgccggaaca gatgcctggc cagcaggacc gctttatcgg cggcgggctg
     4261 aaatatcctt atgtcgaagg attgcgtctc gacgaagcaa tgcatccgct cacactgatg
     4321 accgtaggtg tttatggcaa ggcgttacca ccgcaaaatg gcgcgccggt gcgactgatt
     4381 gtgccgtgga aatatggctt taaagggatt aaatcgatcg tcagtattaa gctgacccgc
     4441 gagcgtccgc caaccacctg gaatctggca gcgcctgacg aatacggttt ttacgccaac
     4501 gttaatccgc atgttgatca cccgcgctgg tcacaggcta ccgaacgatt tattggttca
     4561 ggcggcattc tcgatgttca gcgccagcca acactactgt ttaatggtta cgccgaccag
     4621 gtggcatcgc tgtatcgtgg cctggatttg cgggagaatt tctaaatgcg tctgacagca
     4681 aaacaagtga tatggctgaa agtttgcctg catcttgccg gattgttgcc gtttctctgg
     4741 ctggtctggg cgatcaatca cggtggactg ggtgccgatc cggtgaaaga tattcagcat
     4801 tttaccggtc gcactgcact gaaatttttg cttgcggcct tgttaatcac acctctggca
     4861 cgctacgcaa aacagccgtt attgatacgc actcgccgcc tgttaggctt atggtgcttt
     4921 gcctgggcga cactgcattt aaccagttac gcattgctgg agttgggcgt gaataatctg
     4981 gcgttattag gtaaggagtt aattacccgg ccttatttaa cgttaggaat tatcagctgg
     5041 gttattttgc ttgctttagc gttcacttca acccagtcga tgcagcgaaa actcggcaaa
     5101 cactggcaac agttgcataa cttcgtctat cttgtcgcga tcctggcccc aatacattat
     5161 ctgtggtcag tgaagattat ttcaccgcag ccactcatct acgccgggct ggctgtactg
     5221 cttttagcct tacggtataa gaagttgctt tcccttttca accggttacg caagcaggcc
     5281 cacaacaaat tatctctata acttcatatt gccgacaaac ttcgcgttat cccggccgga
     5341 tgcggcgcga gcgccctatc cggcccacac agatcgcatc aagttatgtt tcgtttgtca
     5401 tcaacctttc atcaccagaa taatttattt tatttcaata agatagttca atacacctga
     5461 gaataccatg ctctcatttc ctaatgagtt gttgcatttt ttcatatgtt acaatataac
     5521 attacacata atgtacatta actctggagg aaattgtttt ggcgattcgt cttcataaac
     5581 tggctgttgc tttaggtgtc tttattgtta gcgctcctgc cttttcgcat ggtcatcatt
     5641 ctcatggcaa acccttaaca gaggtcgaac aaaaagctgc taatggtgtt tttgatgata
     5701 ccaatgtaca aaaccgaacg ctcagtgact gggatggagt ctggcaatcc gtttaccctt
     5761 tactgcaaag tggcaaactt gatcccgtct ttcagaagaa agcggatgca gataaaacca
     5821 aaacatttgc tgaaattaaa gattattatc gcaaaggtta tccgacagat atcgaaatga
     5881 ttggcattga ggacggcatt gtcgaattcc atagaaataa tgaaacaaca tcctgtaaat
     5941 atgattacga tggatacaaa atactcacct ataaatcagg caagaaaggc gttcgttact
     6001 tattcgaatg taaagatcct gaaagcaaag cccctaaata tatacaattt agcgatcata
     6061 taattgcacc acgaaaatca tctcattttc acatttttat gggtaatgat tcccagcaat
     6121 ctttattgaa tgaaatggaa aactggccaa cgtattatcc ctatcagttg agtagcgaag
     6181 aagtggtcga ggagatgatg tctcattgat ttttttgcgc tgacttcagt tatttaacag
     6241 aacagtgcct ggtagtaaca acaataattt accaagacaa ataagtaaga caaactcaga
     6301 cacgctcata cttgagtcgt gtctttttta ttatgtctgt agaaaaaaac tgagaaaaag
     6361 gctaacaata aatacacaac tcagccatac agactggcag cgcaaatgtt tccaggagca
     6421 agtcatctgt catggtaaag ctaaccagaa atataggtta attttggtgt ttaaaccttt
     6481 tttacttgtt agtatgatac aaaggctttc aaaaaagctg ggaaattcaa caactcttgg
     6541 aaattatgga ctttgtcccg cggctttaat ctgacggccg cgttcctttt ttggctcaat
     6601 tattgacccc ttctctacgc aacttcagtt cccgccagca actttgcggc agtttcatag
     6661 gatcaatgtc taaaagaata atggtgaccg ataagaaaac gactgaataa ctgtagattt
     6721 tcgctcgaaa ccttcctgtc agctccatag cgaatcaagc gctgaatgtc acagtatcga
     6781 acagaaaaca gtgacgatct aacccttcaa gaatattcta cgattgttct gtttaggaaa
     6841 agcaaggcgg gaagtcggga gataagtcat tgataaaatg gcggagagag ggggatttga
     6901 acccccggta gagttgcccc tactccggtt ttcgagaccg atgatacaga gtcgaaaaat
     6961 caaagagtta aaaatttttc ttggaaaaaa tgtcttaaaa tcgtacacga aaaatcaatg
     7021 aattacataa cattaaagaa gaatattcca agccttcttg agtccaccac ctgttttcag
     7081 gtgtcattca gacgtgattt gccccagatt gggaaagcac gcttttttct cagcttattc
     7141 cgttttgcct gtgtgttgaa taattccact gcagttatat aaaatcggta acggctggaa
     7201 atcattcaat actcgcacta tcgaaagttt accagccaac cgcagcacgt cctgcatacg
     7261 tcgtgtctgc ggtttttctt tttcgcttac attgtgtctg gttcttccgg ccactcaata
     7321 tcaggtgcag ttgatgtatc aacacggttc agcaacaccc gatacttctt ccaggcttcc
     7381 agcaacgagt tttcttcctc cgttgcgatc tccagatcta cagcatcctg aagtggcgca
     7441 atatgctcac tgaatccctg gatgtagaac tgtgtggtga cggtcttcca gccattcggc
     7501 tcctgctgta tcgaagcata ccaggctatt tcaatatcgc tatgctgcgg cagcatttaa
     7561 ccccttgtaa ttcatcgcca taattgattt aattcacaaa taaaactata atatggtgaa
     7621 attaatgaaa aaaaacacag atgatggggc taaaatttac acaccactta ccctaaagct
     7681 ttatgactgg tgggttttgg gagtatcaaa tcggcttgca tggggatgtc ctacaaagga
     7741 acaccttctt ccacactttc tggaacattt aggtaacaac catctggata ttggtgttgg
     7801 aactgggttt taccttactc acgtacctga gagtagtctg atatctttaa tggatttgaa
     7861 cgaagctagc ctgaacgcgg catctacaag ggctggggaa tcaaaaatta aacataaaat
     7921 tagccatgat gtttttgaac cttatcctgc ggcgttacat ggtcaatttg attccatttc
     7981 catgttttac cttcttcact gcctgcctgg aaatatatct acaaaaagct gtgtaatacg
     8041 caatgcggcg caggccttaa ctgatgatgg aactctatac ggagccacaa ttcttggcga
     8101 tggagttgtg cacaatagct tcggtcaaaa actgatgcgc atttacaatc agaaaggcat
     8161 cttttcaaac acaaaagatt ccgaagaagg cttaacacat atactctcag agcatttcga
     8221 gaatgttaaa accaaggttc aaggtactgt agtaatgttt tccgcttcag ggaaaaaata
     8281 gcatccaacc gcagcacgtt cttgcttaag acgtgctacg gtttccttta cgatttgggg
     8341 ttgcgacttt acctctattg ataatgcatt ccggcagaac gttcaaatat gaacgtacga
     8401 tatttaacta accgaaaaac aaaatatagc acaggcgaga catttaccat ctaagaaaat
     8461 agtatcgttt ttttacagtt aaatcaacat cacttcctta aaatgaaaac aataaatttc
     8521 aatcctgaaa cattttattt cagcttatta ttccccagta tcacatttta tcaggatatc
     8581 cttctgcgag gttattatgc ttcctgtaaa taatcccccc ctatccactg gaaacgtctc
     8641 tttttacaga actacatcaa tcgacaatgt tcacaataat tatctctccg aatgggttga
     8701 atggactaaa aacagcattt ccggagaaaa cagggaaact gcttttaccc ggctccaatt
     8761 atgtctggag aacagtgaaa catcgttgga cttatcttgt ttaggtctca gatctctacc
     8821 acgattgcct gacaatcttg atgaaattaa tgtaagcaat aaccaactat caatgctccc
     8881 cgagctacca agggcattga aagagctgaa tgcaagcagt aatcaattat ctgcacttcc
     8941 tgaattacca gtgtcgctgg aatatataaa tgtgagtgat aaccatttgt tcgcacttcc
     9001 tgaattacct gcgtcactag aatatattaa tgtaagtgac aatcacctgt ctgtacttcc
     9061 gaggttacca atgtcattgg aattacttga tgcagccaga aatgctttgg aagtaatacc
     9121 agattttcca gaaagagatg atcatattat aagaatattc tggcttaatc agaaccggat
     9181 cacggcaatt ccggaaagca tacttggcct cagttctgat agcgttgtca atcttagaga
     9241 aaatcaacta tctcccagaa taatgcaaac tttgttacaa caaaccgccc a
//
LOCUS       DAJTQQ010000132         9289 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000132 DAJTQQ010000000
VERSION     DAJTQQ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9289
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.132"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..999
                     /locus_tag="OO309_003604"
     CDS             <1..999
                     /locus_tag="OO309_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848605.1"
                     /translation="DILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVL
                     SGIIGNVPKNNKPKTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVE
                     YVPLACEAHSHRSVALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKN
                     RMAELFNQSDISVRNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGD
                     TNVAVVMGCKSAKDRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPE
                     ELMMLKDLFDIRVLHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            1678..1896
                     /locus_tag="OO309_003605"
     CDS             1678..1896
                     /locus_tag="OO309_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR5848606.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2114..2356
                     /locus_tag="OO309_003606"
     CDS             2114..2356
                     /locus_tag="OO309_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848607.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(2686..3615)
                     /gene="fdhE"
                     /locus_tag="OO309_003607"
     CDS             complement(2686..3615)
                     /gene="fdhE"
                     /locus_tag="OO309_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR5848608.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(3612..4247)
                     /gene="fdoI"
                     /locus_tag="OO309_003608"
     CDS             complement(3612..4247)
                     /gene="fdoI"
                     /locus_tag="OO309_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5848609.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4244..5146)
                     /gene="fdoH"
                     /locus_tag="OO309_003609"
     CDS             complement(4244..5146)
                     /gene="fdoH"
                     /locus_tag="OO309_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR5848610.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5159..8209)
                     /gene="fdnG"
                     /locus_tag="OO309_003610"
     CDS             complement(5159..8209)
                     /gene="fdnG"
                     /locus_tag="OO309_003610"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7622..7624),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5848611.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            8403..9236
                     /gene="fdhD"
                     /locus_tag="OO309_003611"
     CDS             8403..9236
                     /gene="fdhD"
                     /locus_tag="OO309_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR5848612.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2259 a         2286 c         2416 g         2328 t
ORIGIN      
        1 gatatacttg cattgcccgt caacgtatat catgcggatt ttaaaattaa aatcaataat
       61 gcagtggaga aggcttcatc aattgtgtta tgtgctgtgc aaaaaaatct ccatgcagca
      121 aggaaggttc ttagcgggat aataggaaat gtgccaaaaa ataataaacc aaaaacaatt
      181 atttttgatt tgcgttttct tagtgcaatt aagcagaaag tttttttggg cagtgaaaaa
      241 ccgttgtttg taaagcatac cgcaatgatg agggaggcat gtaaggaatt accgcagtca
      301 gtggaatatg ttcctttagc ctgtgaagcg catagccatc gcagcgtcgc ccttgccaaa
      361 accgtggctg cgcccatcat ggcgacaggt gtgggcttgg cattaatgta tgcaacatca
      421 tcaggaccgt attatgcgca aagtctttct ggttcgccgg atatcgagat gataaaaaat
      481 cgcatggctg aattgtttaa ccagtcggat atttctgtgc gcaacaaata tagcccggct
      541 tttgataaat ggaatgaact ggtagataaa ttaaatcacg aaagaaatac agttccgttt
      601 gcgtgtttga ccaccatttt agcgacggcg ataaatgaaa ctcttgaggg ggatacgaat
      661 gttgccgttg tgatggggtg taaatctgcg aaagatcgca cgatatccat tgtccttggc
      721 aactcgatgt tgcaaacact ccttgaaaaa cgcctcgccg atagtggaga aatagaaaat
      781 ctttttgatc aacagggata ttttagttgc gattcattaa cacccgaaga attaatgatg
      841 ttaaaagacc tttttgatat tcgagttctt catgtctcta acaaattcaa tgttggattg
      901 caaggtaata tcaacactga tgttttacaa gattcgtttt ttaaaaatgt cgatttcatt
      961 catgaatata actcgtatac cgtagggatg ggagtctgac gagtaaaact cattaggatt
     1021 atcagaattc ggacaatgca ttgcaaaaat gacatacaaa gagagatgtg tgcaggtact
     1081 tagccgcgag gggcatggcg aagccataca gaaagtacca gtattcagaa ctccagtgaa
     1141 agcggcatag tggccgcctg ctcaggagtt ttgctatgac ccgtaatcgt gctgaacgtc
     1201 gccaccctat gatgcgatta aaaaaactca tcgtcgcgct gatgattgac gtcaatgcta
     1261 tcggatgatg ccgggggtgt tccggaagga attccttgtt tcccgaactg ttaccaacaa
     1321 aattataacg atgagcaagt aaagaccggt tggtggcagt actgaccggt ctgtgatgaa
     1381 agattaattt caccttaaat cctggtgctg ttaggatgct gacgtttttc ggctacacca
     1441 ttcagattat gtaaataaat atccctgaat ataatatatt catagcaccc ataactcatc
     1501 ctgggcgata actaaaccat ttttgagtaa taaatgaaac aactgcaacg ctcttattat
     1561 ttctggaaaa cgcctcgcaa aaacaaaatg acatgcaaaa atccgcattt ttttaaatgc
     1621 ttttccggcg taattttcgc acatacatta atgagtaata tatgtaaggt ggtggtaatg
     1681 gcgatgaata cggtttttct tcatttatca gaagaggcaa ttaaacggct gaacaagctt
     1741 cggggctggc gtaaggtttc acgatctgca attttacgcg aagcggttga gcaatatctg
     1801 gagcggcagc aatttccggt gcgtaaagca aagggaggca ggcaaaaggg ggaggtagtt
     1861 ggtgttgatg atcagtgtaa ggagcataag gaatgaaaaa aatttttcat tcttgaatat
     1921 aaaaaacaga tgcccttatt ctggtattaa tacaaggctg ttttacttga acttataata
     1981 actgcaactg ttacatcgta tctggaaaac gcctcgcaaa aataaaaaat gatgcgtaaa
     2041 tgagctttct ttaaagattc ggcaagggta cttttctctc catagattca aaaatgatat
     2101 tggcgagata tttatgaact ctctggcagg tatagatatg ggcagaattt tactcgattt
     2161 atcgaatgag gtgattaagc aacttgatga tctcgaggtg cagcgtaatc ttcctcgtgc
     2221 ggacctatta agggaagcgg tagatcaata cctgataaat caatcgcaaa cagcaagaac
     2281 cagtgttcct ggcatctggc aagggtgtga ggaagatggt gtcgaatatc agcgtaagct
     2341 gcgcgaggaa tggtaatggg aaaaaatgat gtcaatcaga ttgctgacaa cgtgcgcgtt
     2401 gttcatgccg gatgcggcgt gaacgcctta tccggcctac aaagtcatgc aaattcaata
     2461 tattgaagtt tattggtagg cctgataagc gcagcgcatc aggcaatcat acgcttgtca
     2521 tcagtgacat acccataacc ttatcgatgt gttggaggga agaacgaggc aaacagacaa
     2581 ttgcggcatg atcttgaatg ggtaacgcgg aatacgagag atggtgtcgt cacaccctat
     2641 gagctgtaat ccgggcgacc tgcgccaccc ggaaaacgtc agagattact ctccttcacc
     2701 cggaaacagg aacgggttaa tggaactacg ggcgtagcct tcttgctcca ttcgcgcgtc
     2761 cagtaccaga gaggcgaggt catctgccac ggcttcaact ttcggttctt tttcctgata
     2821 aagaatcttc aggtaagtgc cgcagtcatc gcagctttcg gctttaatcg cggcctgttc
     2881 gtcatccagc gaccagtaat gcagtttgcc gctctgctcg cagttgctgc attttacgcg
     2941 cactacgtgc cattcggttt cacacaggtt gcagtgcagg taacgcagac cctgagtggt
     3001 gccaatttgc accatgctgg acaccggcat agagccacat accgggcaat attgacgttg
     3061 ttcgccgtat tcagcgcggg ctttgccggg gatcagattg gccatctgcg cccagtagag
     3121 cgacagcgca gcccagataa acggcgcttt atcgctgctg acggacgaga aatcagaggc
     3181 aaacagcgcg ctggccatat cttccagctc ctgagtcgat gccttctcca gattctcaat
     3241 cactgccagc gccgggccgc tcatttcagg tttcagctca gcaattagcg ccatcagcag
     3301 cttttgccag tgcttatcac gcggcagaac gtgaatatcc agcgggggct tgccctgtgc
     3361 gctggcttct ttaatgcgcg cggtcagatc catctccagc ggatggtcgt acagcaccac
     3421 tttctgggcg tgggcgataa gcgcagcaaa gcgcaggtaa tcacccagcg gattattttc
     3481 tgccagctcg cgcagacgct cggcgcggcg gttgtataaa ttcttgagcc gagggaacaa
     3541 taacggcgga atcatatccg ccgtacgttt ctcgctcgaa cccagctcat cttgcgggat
     3601 tatgcgaata ctcattcagc tttcttttcc gttgtcttgc ggacctcacg gtaccagcgc
     3661 gggtgatgtt tcttcgccca ggcgctggta acccatcctt ccaccatggc ggtaatcgtg
     3721 cctttcaccc aaagggcggc gtagatatgc accatgataa ccacaattaa cgccactgcg
     3781 gcaaatgaat gcagcattaa cgcgaatcgg atcaccggga ttgagaaagc aggcgcaaaa
     3841 taaggacgcc agataatcac gccgctcacc agcagcagaa ccaggaaaat aatcgccgcc
     3901 cagaaaacgc atttctgacc gaagttataa cgcccggtgt cacctacttc ctcgttgacg
     3961 acgatcttac gaatattctt cgcccaaaag atatcatccc gattgattag gttttgatgc
     4021 cagtaacgga aaaacatgat gatgaacgag gcaaacataa ccacgccgac aaacgggtgc
     4081 agaattcgcg ccagctgcgg tgtgcccatg atttgcatca accagttgaa ggacgggaac
     4141 aaaaagccca gcccgctcac cgccgccagg atgaagcaga aggcggtgat ccagtggttg
     4201 atacgttccg gcgcggtgta gcgcacgatg gtgtcacgtc gtttcatttg cgctcctcgt
     4261 ctttctcttc gtgcagatta ttctcttcct catccgcacg gttcggaccg acacccacgt
     4321 agtggaagat actggccgca aaggtagccg caaagccaac agccgcgagc ggtttccaga
     4381 tgcctttcca gaatttcacg gtttcgctga tttccgggtt ctccggcaag ccatgataca
     4441 gatttggctt gtcagcatgg tgcagcacgt acatgacgtg tgtaccaccg acgcctgccg
     4501 gatcgtacag acccgcattg tcgtaaccgc gggttttcag ctcagcaacg cgctcgctcg
     4561 ccagcgtttt catcgactct ttcgtgccga agtgaatcgc gcccgttggg caggtcttca
     4621 cgcaggctgg ttcttgccca accaccacgc ggtcaacgca cagcgtacat ttgtagacgc
     4681 ggttgtcttc cgggttgagg cgcggaatgt cgaacggaca gcccgcaatg caataaccgc
     4741 agccaatgca ctgctcggac tggaagtcga cgataccgtt ggcatactga ataattgcac
     4801 cttccgccgg gcacgctttc aggcagcctg gatcggaaca gtgcatacag ccgtctttgc
     4861 ggatcagcca ttccagtttg tcgttctgct ccacttccga gaagcgcatt accgtccacg
     4921 atttggcgct taaatcattg gggttgtcgt acaccccaat gttattgccg acggtatcgc
     4981 gaatgtcgtt ccactctgaa cacgccacct gacaggcttt acagccgata caggtggtaa
     5041 cgtcgatgag tttcgccact tcttcctgga agtcccgcgc ctgaggcgcg ggggtcagac
     5101 cgttagtcgc ggaacgacga atgatatctt gagattgata agccatatgt cgtctccgtt
     5161 acaccttttc cacattcaca aggaaggact taaactccgg cgtctgcgtg ttcgcatcac
     5221 caacgaatgg cgttaaagta ttggcaataa agcctttttt agctacgcct tcatagcccc
     5281 agtgaatcgg aataccgatg gtatcgatat ctttgccgtt cgctttcagc gtgcgaatac
     5341 gtttggtcac caccgctttg gctttgatat agccacggtt ggaggagact ttcacggtat
     5401 cgccctgggc aatgccaagt ttattcgcca gcgactcccc gatttccaca aactgctctg
     5461 gctgcaaaat cgcgttcaac agcgcgtgtt tggtccagta gtggaagtgc tcggtcagac
     5521 gataggtggt tccgacatac gggaacttat cggctttacc caatgcttcg gcgtcgtctt
     5581 taaagatacg tgcagctggg ttcgagataa cgtttggatg cagcgggtta gttcccagcg
     5641 gcgtttcaaa cggctcgtag tgttccggga acggaccttc cgccatctta tcgagggcaa
     5701 acagacgccc catgccttcc tgctgcatga taaacggccc gacgccgcta cccggaggcg
     5761 ctgcgctgta gtccggaata tcccagccgg tccacttagt gccgtcccat ttcagcaact
     5821 gacgcttcgg atcccacggg ttaccctgcg gatctgcgga ggcgcggtta tacagaatgc
     5881 ggcggttaag cggccatgcc catgcccagc ccagcgtgtt accgaggcca gacggatcgg
     5941 cgttatcacg acgcgccatc tggttgcctt ccggcgtcca gctaccggcg aaaatccagc
     6001 agccacagga ggttgtaccg tcatcgcgca attgggcgaa cgagctaagt tgttggcctt
     6061 tcttgacgat aaccgcaccg gttgccggat cggtaatatc ggccagcgcc ttaccgttgc
     6121 tctccatcgc tacttcttcc gacttcggct catgcggaat ggcgtagttc caggtcatgt
     6181 tcagcacctg atccgggttc gcgccaccct gttcggcata catcttgcgc aagcgcagga
     6241 agataccgga gaggatctca ccgtcagtca gcgcaatccc cggggcgtcc gcacctttcc
     6301 agtgccactg caaccagcga cctgagttaa cgattgaacc gttctcttct gcgaagcagg
     6361 tcgatggcag acggaacact tcggtctgga tcttcgacga gtcaacttcg ttcagctcac
     6421 cgtggttctg ccagaagtta gaggtttcag tgttcagtgg gtcgatggtg acgaggaact
     6481 tcagtttcga cagacaaccg atcactttgt ttttgttcgg gaatgaggca acagggttaa
     6541 agccctggca gatatagcca ttgaccttgc cctctttcat catctcgaag tattgcagga
     6601 cgtcgtagcc tttatcccac ttcggcaacc agtcaaagcc ccagctattt tccgccgtcg
     6661 ctttatcacc aaagaaggcc ttcatcatag agacgaagaa tttcgggtag ttgccccagt
     6721 agtttacctg gccttccagc agcggttttg gcgtgttggc ggtaaggtag gtttgcagat
     6781 cggtctgctt ctcgcttggc agcgtcatgt aacctggcag actctgcgac agtagcccca
     6841 ggtccgtcag cccctgaata ttggagtgac cgcgcagggc gttaacgccg ccgccggcca
     6901 tccccatatt gccgagcagc agctggatca tcgccatcgt acgaatgttt tgcgcaccaa
     6961 cggagtgttg cgtccagccg agggcataca ggaacgaggc agttttatcg tgagcactgg
     7021 tttctgcgat gtattcgcag actttcagga acgcgtcttt tggcgtacca cagatgtttt
     7081 caaccacatc tggcgtgtag cgggaaacgt gctgtttcag caagttccac acgcagcgcg
     7141 gatgttgcag cgtggtatcg cgtttggcga agccgttttc gtccagttca taagtccagg
     7201 aggatttatc gtacttgcgt ttttccgcgt cgtagccggt gaacaggcca tcttcaaagc
     7261 cgtaatcctc acgcacgatc aggctggcgt tggtataggc ttcggtgtat tcgtggttga
     7321 atttttcatt gttcagcagg tacagcaata cgcctgacag gaaagcaatg tcagtaccgg
     7381 aacgaatagg ggcatagtag tcagctaccg ccgccgtacg cgtaaagcga ggatcgatca
     7441 caatcagctt cgcgccgttg tgaattttgg cttccatcgc ccagcggaac ccgaccgggt
     7501 gagcttcagc ggcgttaccg cccatcacca cgacgaggtt ggcgttcttg atgtcgaccc
     7561 agtggttggt catcgcaccg cgaccaaatg ttggagcaag acttgctacc gttggtccgt
     7621 gtcagacacg cgcctggttg tcgaccgcga gcatacccag cgcgcgggag aatttttgcg
     7681 ttaaatagcc ggtttcgtta ctcgacgcgg aagcacacag catcccggtg gagagccagc
     7741 ggttaacagt cacgccttcg gcgttttgcg caatgtagtt agcatcgcgg tcttctttca
     7801 tcagtttggc gatgcgatca aacgcctctt cccaactgat ttgctgccat ttatcagaac
     7861 ctggcgcacg gtattccgga aacttcagac ggctttcgga gtggatgaaa tccaccaggc
     7921 cagcgccttt cggacaaagt gcaccgcggt tgaccgggtg atccggatcg ccttcgatat
     7981 ggaagataga tgctttggcg ttttttgctc cgtcaccgag gctgtacatc aacagcccac
     8041 agcctacgga acaataggtg caggtattac gggtttcgcg ggtgcgcagc agtttatact
     8101 gccgggtttc cgcgagtgct acgctgggtg caaaacccag tgccgctgcc gtggtgcctg
     8161 ccataccgcc agcgcagatc ttaaagaact gccttctgct gacctgcatg gattgctcct
     8221 tgtttcgaca ttgtcacgtc ccatttacat tcgcttgctg cgtgtgcagg gagtgggagt
     8281 tatttttctt tgcggaaggg gccgcaaagg tccagaattg gctcaatttc cctccatcta
     8341 ggaagggttt gtaacagaat accataatgt tggtgtgtat gttcttatct ggttaagaga
     8401 aagtgaaaaa aacacagcga aaagaaatcg aaaatgtgac aaatatcaca ggtgttcgtc
     8461 aaattgagtt atggcggcgt gatgatttac aacacccacg gttagatgag gtcgcggaag
     8521 aagttcccgt tgcgctggtc tacaacggca tttcgcatgt ggtgatgatg gcgtcgccca
     8581 aagaccttga gtactttgcg ctcggttttt cgctttccga ggggataata gaaagtccgc
     8641 gcgatatctt cggcatggat gtcgttcctt cctgtaatgg tcttgaagta caaattgagc
     8701 tttccagccg ccgctttatg gggttgaagg agcgccgccg ggcgctggcg ggacgtacgg
     8761 gctgtggcgt atgcggcgtg gagcaactta atgacatcgg aaaaccggtg cagccgctac
     8821 cgttcaccca gacgtttgat ctcaacaaac tggatgatgc attacgtcat ctcaacgatt
     8881 ttcagccagt ggggcgactg actggttgta ctcacgccgc tgcctggatg ttgccatctg
     8941 gcgaactggt cggcgggcat gaagacgtgg gtcgccatgt ggcgctggac aaactgttag
     9001 gccgccggtc acaagaaggg gaaagctggc agcaaggtgc ggtactggtt tccagtcgtg
     9061 ccagttatga aatggtgcaa aagtcggcga tgtgcggcgt agagattttg tttgcggtgt
     9121 ctgccgcgac cacgcttgct gtagaagtgg ctgagcgctg taatctgaca ctggtaggtt
     9181 tttgtaaacc gggtcgggca acggtttata cccatccgca gcgtttgagc aattaattaa
     9241 aataagtcct gcgatatata ttaaattgca ggacttattc atttcgtga
//
LOCUS       DAJTQQ010000133         9167 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000133 DAJTQQ010000000
VERSION     DAJTQQ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9167
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.133"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..925
                     /locus_tag="OO309_003612"
     CDS             <1..925
                     /locus_tag="OO309_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5848613.1"
                     /translation="LGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAP
                     PALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRP
                     QLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAI
                     PFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAV
                     AFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDT
                     THSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1177..2094
                     /gene="oxyR"
                     /locus_tag="OO309_003613"
     CDS             1177..2094
                     /gene="oxyR"
                     /locus_tag="OO309_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR5848614.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2077..3477)
                     /gene="sthA"
                     /locus_tag="OO309_003614"
     CDS             complement(2077..3477)
                     /gene="sthA"
                     /locus_tag="OO309_003614"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR5848615.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3798..4448
                     /gene="fabR"
                     /locus_tag="OO309_003615"
     CDS             3798..4448
                     /gene="fabR"
                     /locus_tag="OO309_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR5848616.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4448..4807
                     /locus_tag="OO309_003616"
     CDS             4448..4807
                     /locus_tag="OO309_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR5848617.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4847..5947)
                     /gene="trmA"
                     /locus_tag="OO309_003617"
     CDS             complement(4847..5947)
                     /gene="trmA"
                     /locus_tag="OO309_003617"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR5848618.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6061..6251
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6316..8160
                     /gene="btuB"
                     /locus_tag="OO309_003618"
     CDS             6316..8160
                     /gene="btuB"
                     /locus_tag="OO309_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR5848619.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8105..8962
                     /gene="murI"
                     /locus_tag="OO309_003619"
     CDS             8105..8962
                     /gene="murI"
                     /locus_tag="OO309_003619"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5848620.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2093 a         2219 c         2475 g         2380 t
ORIGIN      
        1 tctgggtatt ggcgaagcgc catttatgcc tgcgggagta aagtcgatca ccgactggta
       61 tgcacaaaaa gaacgcggca cggcgctggg gatttttaac tcatctaccg ttatcggtca
      121 ggctatcgcg cctcctgctc ttgtattgat gcagctggca tggggctggc ggacgatgtt
      181 cgttatcatc ggcgtggcag ggattctggt tgggatctgt tggtacgcgt ggtatcgcaa
      241 ccgggcgcag tttgtcctga ctgacgaaga acggacgtat ctctccgcgt cggttaaacc
      301 gcgtccacag ctgcaattta gcgagtggct ggcgctgttt aagcatcgga caacctgggg
      361 gatgatttta ggtttctctg gcgtcaatta taccggttgg ctttacattg cgtggttacc
      421 cggttatttg caagccgaac aaggtttcag tctggcgaaa accggttggg tagcggcgat
      481 tcctttcctc gcggcggcgg tcgggatgtg ggttaacggt attgtggtcg atcggctggc
      541 gaaaaaaggc tacgacctgg cgaagacgcg taaaacggct attgtctgcg gtttgatgat
      601 gtcggcatta ggcacgttgc tggtcgtgca atcttcctcg ccagctcagg cggtagcgtt
      661 tatctcaatg gcgctgttct gcgtgcattt cgcgggaacg tctgcatggg gactggtgca
      721 ggtgatggtg tcagaaacaa aagtggcttc catcgccggg attcaaaact ttggcagttt
      781 tgtttttgct tcctttgctc cgatcgtaac cggttgggta gtggatacca cacactcgtt
      841 taatctggcg ctggttatcg cggcctgtgt gacgttcacc ggggcgctgt gttacttctt
      901 tattgtcaaa gatcgcattg agtaatcgtt actatcaggc tctcttgctg tgggcctgta
      961 gaataaaaaa aagcggatcc tggagatccg caaaagttca cgttggcttt agttattcga
     1021 gttgagaaac tctcgaaacg ggcagtgact tcaagggtta aaagaggtgc cgctccgttt
     1081 ctgtgagcaa ttatcagtca gaatgcttga tagggataat cgttcattgc tattctacct
     1141 atcgccatga actatcgtgg cgatggagga tggataatga atattcgtga tcttgagtac
     1201 ctggtggcat tggctgaaca ccgccatttt cggcgtgcgg cagattcctg ccacgttagc
     1261 cagccgacgc ttagcggcca aattcgtaag ctggaagatg agctgggcgt gatgttgctg
     1321 gagcggacca gccgtaaagt gttgttcacc caggcgggaa tgctgctggt ggatcaggcg
     1381 cgtaccgtgc tgcgtgaggt gaaagtcctt aaagagatgg caagccagca gggtgagacg
     1441 atgtccggac cgctgcatat tggtttgatt cctacggttg gaccatacct gctaccgcat
     1501 attatcccga tgctgcacca gacatttcca aagctggaaa tgtatctgca tgaagcacag
     1561 acccaccagt tactggcgca actggacagc ggcaaactcg attgcgtgat cctcgcgctg
     1621 gtgaaagaga gcgaagcatt cattgaagtg ccgttgtttg atgagccaat gttgctggct
     1681 atctatgaag atcacccgtg ggcgaaccgc gaatgcgtac tgatggccga tctggcaggg
     1741 gaaaaactgc tgatgctgga agatggtcac tgtttgcgcg atcaggcaat gggtttctgc
     1801 tttgaagccg gggcggatga agatacacac ttccgcgcga ccagcctgga aactctgcgc
     1861 aacatggtgg cggcaggtag cgggatcact ttattgccag cgctggctgt gccgccggag
     1921 cgcaaacgcg atggggttgt ttatctgcca tgcattaagc cggaaccatg ccgcactatt
     1981 ggcctggttt atcgtcctgg ctcaccgctg cgcagccgct atgagcagct ggcagaggcc
     2041 atccgcgcaa gaatggatgg ccatttcgat aaagtgttaa aacaggcggt ttaaaccgtt
     2101 taacgcagct acccgatagg cttccgccat cgtcgggtag ttaaaggtgg tgttgacgaa
     2161 gtactcaata gtgttgccgc cacctttctg ttccataatc gcctgaccga tatgaataat
     2221 ttcggcagcg cgctcgccaa agcagtgaat acccagaatc tcttttgttt cccgatggaa
     2281 caaaattttc agcgtgccca cgttcatgcc gacgatttgt gcacgtgcca gatgtttgaa
     2341 ctgggcgcgg cccacttcat acggcacttt catcgcggtc agctgctgtt cggttttgcc
     2401 cacagagctg atttccggga tggtgtaaat gccggtaggg atatcttcaa tcagatgtgc
     2461 ggtggcttcg ccttttacca gcgcctgcgc ggcaatgcgc ccctggtcat aggccgctga
     2521 tgccaggctc ggataaccaa tcacgtcgcc caccgcgtaa acgtgcggct gtgcggtctg
     2581 atacatgctg ttgaccttca gctgtccgcg actgtcagtt tccagcccaa tgttctgtaa
     2641 cgccagcgaa tcggtattac cggtacgacc gttggcatag agcaggcagt cagctttcag
     2701 ttttttaccc gacttcagat gcatgatcac accgtcgtca cagctttcga tcttctcgta
     2761 ctcttcgttg tgacgaatca ctacgccact gttccagaag tggtaggaga gagaatctga
     2821 catctcttga tcgagaaatg ccagcaggcg atcgcgggtg ttgatcagat ccacttttac
     2881 atccataccg cggaagatcg acgcatattc acagccgatc actccagcac catagataag
     2941 tacatggcgc ggttcgtggt gcatgctaag aattgagtcg ctgtcgtaaa tgcgtggatg
     3001 ggtgaaatca acatctgttg gatgatatgg acgagagccg caggcaataa caaatttttc
     3061 agcggttagt gtttcaacgc tgccgtccgg gcaatccagc gccaacgtat gctcgtcaac
     3121 aaagcgagcg ttcccctgca atatttcaca gtgattacgt tcgtaaaatc cctgacgcat
     3181 gcgcgtttgt tgattaatca cgttatcggc atggttaagg atatcggcaa aagaagagcg
     3241 gagcagtcgg gaatggtcgc tgtatagtgg gttttgattg aattctataa tgcggctgac
     3301 ggcgtgacgg agagctttcg acgggatggt gccccagtgg gtgcaaccgc cgccaacatt
     3361 ttgataacgc tcgataactg cgacgcgcgc accttgctta accaggccca ttgcagcgcc
     3421 ttcgccgccg gggccggaac ctattactat ggcatcgtaa tcgtaggaat gtggcatggt
     3481 agggcttacc tgttcttata cataaaagca acagaatggt aacattctat cgcgggtaag
     3541 ccaattgatc cccgtcattt atctggctat atcctgagcg gcctttgctt tgtctgtttc
     3601 ttacttttgc cctgacgttt tattggattt ttatcgacga tactctccgt ttaagcggca
     3661 ggtttccgct gtacgtaaaa gaaccggcca gtaaatatgt tttattgcgt taccgttcat
     3721 tcacaatact ggagcaatcc agtatgttca ttctctggta tagtgccagc agtacttttg
     3781 gcaaggattc agacatcgtg atgggcgtaa gagcgcaaca aaaagaaaaa acccgccgtt
     3841 cgctggtgga agccgcattt agccaattaa gtgctgaacg cagcttcgcc agcctgagtt
     3901 tgcgtgaagt ggcgcgtgaa gcgggcattg ctcccacctc tttttatcgg catttccgcg
     3961 acgtagacga actgggtctg accatggttg atgagagcgg tttaatgcta cgccaactca
     4021 tgcgccaggc gcgtcagcgt atcgccaaag gcgggagtgt gatccgtacc tcggtctcca
     4081 catttatgga gttcatcggt aataatccta acgccttccg gttattattg cgggaacgct
     4141 ccggcacctc cgctgcgttt cgtgccgccg ttgcgcgtga aattcagcac ttcattgcgg
     4201 aacttgcgga ctatctggaa ctcgaaaacc atatgccgcg tgcgtttact gaagcgcaag
     4261 ccgaagcaat ggtgacaatt gtcttcagtg cgggtgccga ggcgttggac gtcggcgtcg
     4321 aacaacgtcg gcaattagaa gagcgactgg tactgcaact gcgaatgatt tcgaaagggg
     4381 cttattactg gtatcgccgt gaacaagaga aaaccgcaat tattccggga aatgtgaagg
     4441 acgagtaatg aaacaagcaa atcaagatag aggtacgctg ctgctggcgt tggttgctgg
     4501 tttatcgatt aatggtactt tcgcagcgct gtttagctcc attgtgccat tttctgtatt
     4561 cccgattatt tccctggtgc tgacggttta ctgcctgcat caacgttatc ttaatcgcac
     4621 catgtcggta ggcttgccgg gtctggcagc tgcctgtttt attctcggcg tactgctgta
     4681 cagcacggta gttcgtgcgg aatatccgga tatcggctct aacttcttcc cggcagtact
     4741 ctccgtcatt atggtgttct ggattggcgc gaagatgcgt aaccgtaagc aggaagttgc
     4801 tgagtaatcg gtatcatgcc ggatgctggc gcatccggca tgggttttac ttcgcggtca
     4861 gtaatacgcc gcactccata tggtgcgtgt aggggaactg atcaaacaga gccagacgtt
     4921 cgaccttgtg cgtctggctt aatgtttcca gatttttgca taacgtttcc ggattgcagg
     4981 agatatacag aatacgtgga tacgcctgca ccattttctc ggtttcactg tccagaccgc
     5041 tgcgcggtgg gtcgacaaaa atggtttcga actgatagct ctttaagtcg atcccttgca
     5101 ggcggttaaa ctcgcgcaca ccattcatcg cctgagtaaa ttcttctgcc gccatacgaa
     5161 taatttgtac gttatcaata tggttagcgg caatgttgta ttgcgcggca gcgactgacg
     5221 gtttggcgat ttcggtggct aatacccgat caaaattgcg cgccagcgct aatgaaaagt
     5281 taccgttgcc gcagtacagc tccagtaaat cgccttttga gcctttggtt acgtccagcg
     5341 cccattccag catctgaata ttcatcgccg cgttcggctg ggtaaagctg ttttctacct
     5401 gacgatagat catctctttc cctgcgaccg gcagacgctc gtcgatgtaa tcttgatcca
     5461 gcgcgatttt ggttttcgtt gcccgaccaa tcagatgcac attcagattc tgcgcgcgca
     5521 gtgcatcgcg cagtgcttcc gcttcctgac gccactcatc atccagcttt ttatggtaca
     5581 gcagggaaac caccgcctga ttactcagcg tggtgaggta atcaatctgg aacaacttgt
     5641 ggcgcagaac gggattatta cgcacacccg caatcatcgc cgtcatcaac tggttgatga
     5701 gttcactggc ggcggggaag ctatccacgc ggatgcggct tttggtttgt tgatcgaaaa
     5761 tgatgtgata caggtcatcg ccatcgtgcc agatgcggaa ctccgcgcgc atccggtaat
     5821 gactgaccgg cgagcgaaac acttccggaa ccaggtcaga aaacggtgcc atcatacttt
     5881 gcaaacgtac cactttttcg gctaactgcg cttcatactg ttctgttgga aggtgttcgg
     5941 gggtcatgat gtatcctgaa aaattaaagt acgcggcgat tgtagggatt gctcatcaga
     6001 tgtccagatc ttgatgaatt cctatttgtg agctacgtct ggacagtaac ttgttacaac
     6061 ctgtagcatc cacttgccgg tcctgtgagt taatagggaa tccagtgcga atctagagct
     6121 gacgcgcagc ggtaaggaaa ggtgcgatga ttgcgttatg cggacactgc catccggtgg
     6181 gaagtcatca tctcttagta tcttagatac ccctccaagc ccgaagacct gccggccaac
     6241 gtcgcatctg gttctcatca tcgcgtaata ttgatgaaac ctgcggcatc cttcttctat
     6301 tgtggatgct ttacaatgat taaaaaagct tcgctgctga cggcgtgttc cgtcacggca
     6361 ttttccgctt gggcacagga taccagcccg gatactctcg tcgttactgc tatccgtttt
     6421 gaacagccgc gcagcactgt gcttgcacca accaccgttg tgacccgtca ggatatcgac
     6481 cgctggcagt cgacctcggt caatgatgtg ctgcgccgtc ttccgggcgt cgatatcacc
     6541 caaaacggcg gttcaggtca gctctcatct atttttattc gcggtacaaa tgccagtcat
     6601 gtgctggtgt taattgatgg cgtccgcctg aatctggcgg gggtgagtgg ttctgccgac
     6661 cttagccagt tccctattgc gcttgtccag cgtattgaat atatccgtgg accgcgctcc
     6721 gctgtttatg gttccgatgc aataggcggg gtggtgaata tcatcacgac gcgcgatgaa
     6781 cccggaacgg aaatttcagc agggtgggga agcaatagtt atcagaacta tgatgtctct
     6841 acgcagcaac aactggggga taaaacacgg gtaacgctgt tgggcgatta tgcccatact
     6901 catggttatg atgttgttgc ctatggtaat accggaacgc aagcgcagac agataacgat
     6961 ggttttttaa gtaaaacgct ttatggggcg ctggagcata actttactga tgcctggagc
     7021 ggctttgttc gcggctatgg ctatgataac cgtaccaatt atgacgcgta ttattctccc
     7081 ggttcaccgt tgctcgatac ccgtaaactc tatagccaaa gttgggacgc cgggctgcgc
     7141 tataacggcg aactgattaa atcacaactc attaccagct atagccatag caaagattac
     7201 aactacgatc cccattatgg tcgttatgat tcgtcggcga cgctcgatga gatgaagcaa
     7261 tacaccgtcc agtgggcaaa caatgtcatc gttggtcacg gtagtattgg tgcgggtgtc
     7321 gactggcaga aacagactac gacgccgggt acaggttatg ttgaggatgg atatgatcaa
     7381 cgtaataccg gcatctatct gaccgggctg caacaagtcg gcgattttac ctttgaaggc
     7441 gcagcacgca gcgacgataa ctcacagttt ggtcgtcatg gaacctggca aaccagcgcc
     7501 ggttgggaat tcatcgaagg ttatcgcttc attgcttcct acgggacatc ttataaggca
     7561 ctaaatctgg ggcaactgta tggcttctac ggaaatccga atctggaccc ggagaaaagc
     7621 aaacagtggg aaggcgcgtt tgaaggctta accgctgggg tgaactggcg tatttccgga
     7681 tatcgtaacg atgtcagtga cttgatcgat tatgatgatc acaccctgaa atattacaac
     7741 gaagggaaag cgcggattaa gggtgtcgag gcgacagcca attttgatac cggaccactg
     7801 acgcatactg tgagttatga ttatgtcgat gcgcgcaatg cgattaccga cacgccgttg
     7861 ttacgccgtg ctaaacagca ggtgaaatac cagctcgact ggcagttgta tgacttcgac
     7921 tggggtatta cttatcagta tttaggcact cgctatgata aggattactc atcttatcct
     7981 tatcaaaccg ttaaaatggg cggtgtgagc ttgtgggatc ttgcggttgc gtatccggtc
     8041 acctctcacc tgacagttcg tggtaaaata gccaacctgt tcgacaaaga ttatgagaca
     8101 gtctatggct accaaactgc aggacgggaa tacaccttgt ctggcagcta caccttctga
     8161 accacgtccc accgtgctgg tgtttgactc cggcgtcggt gggttgtcgg tctatgacga
     8221 gatccggcat ctcttaccgg atctccatta catttatgct ttcgataacg tcgctttccc
     8281 gtatggcgaa aaaagcgaag cgtttattgt tgagcgagtg gtggcaattg tcaccgcggt
     8341 gcaagaacgt tatccccttg cgctggctgt ggtcgcttgc aacaccgcca gtaccgtttc
     8401 acttcctgca ttacgcgaaa agttcgactt cccggttgtt ggtgtcgtgc cggcgattaa
     8461 acctgctgca cgtctgacgg caaatggcat tgtcggatta ctggcaaccc gcggaacagt
     8521 taaacgttct tatactcatg agctgatcgc gcgtttcgct aatgaatgcc agatagaaat
     8581 gctgggctcg gcagagatgg ttgagttggc tgaagcgaag ctgcatggcg aagatgtttc
     8641 tctggatgca ctaaaacgta tcctacgccc gtggttaaga atgaaagagc cgccagatac
     8701 cgttgtattg ggttgcaccc atttccctct actacaagaa gaactgttac aagtgctgcc
     8761 agagggaacc cggctggtgg attctggcgc agcgattgct cgccgaacgg cctggttgtt
     8821 agaacatgaa gccccggatg caaaatctgc cgatgcgaat attgcctttt gtatggcaat
     8881 gacgccagaa gctgaacaat tattgcccgt tttacagcgt tacggcttcg aaacgctcga
     8941 aaaactggca gttttaggct gatttggttg aatgttgcgc ggtcggaaaa ttattttaaa
     9001 tttcctcttg tcaggccgga ataactccct ataatgcgcc accactgaca cggaacaacg
     9061 gcaaacacgc cgacgggtca gcggggttct cctgagaatt ccggcagaga aagcaaaaat
     9121 aaatgcttga ctctgtagcg ggaaagcgta ttatgtacac cccgcgc
//
LOCUS       DAJTQQ010000134         9144 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000134 DAJTQQ010000000
VERSION     DAJTQQ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.134"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..856)
                     /locus_tag="OO309_003620"
     CDS             complement(<1..856)
                     /locus_tag="OO309_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCR5848621.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLP"
     gene            916..1194
                     /locus_tag="OO309_003621"
     CDS             916..1194
                     /locus_tag="OO309_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848622.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1195..1722)
                     /gene="gadE"
                     /locus_tag="OO309_003622"
     CDS             complement(1195..1722)
                     /gene="gadE"
                     /locus_tag="OO309_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR5848623.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2521..3093)
                     /gene="hdeD"
                     /locus_tag="OO309_003623"
     CDS             complement(2521..3093)
                     /gene="hdeD"
                     /locus_tag="OO309_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR5848624.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3348..3680
                     /gene="hdeA"
                     /locus_tag="OO309_003624"
     CDS             3348..3680
                     /gene="hdeA"
                     /locus_tag="OO309_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR5848625.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3796..4122
                     /gene="hdeB"
                     /locus_tag="OO309_003625"
     CDS             3796..4122
                     /gene="hdeB"
                     /locus_tag="OO309_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR5848626.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4186..4832
                     /locus_tag="OO309_003626"
                     /pseudo
     CDS             4186..4832
                     /locus_tag="OO309_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4874..5404)
                     /locus_tag="OO309_003627"
     CDS             complement(4874..5404)
                     /locus_tag="OO309_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5848627.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5560..6126)
                     /gene="slp"
                     /locus_tag="OO309_003628"
     CDS             complement(5560..6126)
                     /gene="slp"
                     /locus_tag="OO309_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR5848628.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6373..7596)
                     /locus_tag="OO309_003629"
     CDS             complement(6373..7596)
                     /locus_tag="OO309_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848629.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIEFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8177..>9144)
                     /locus_tag="OO309_003630"
     CDS             complement(8177..>9144)
                     /locus_tag="OO309_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5848630.1"
                     /translation="EMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGRG
                     YLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTFG
                     WKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKV
                     SCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIY
                     GFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALI
                     IGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2669 a         1812 c         1915 g         2748 t
ORIGIN      
        1 caggcagcaa gtcaccattt gggttgggga aaatcgcccg taacgtcacg gagcccgtgg
       61 tttcatcaac cgtcgggtcg gagaatttca gcgtgccggt ctggctgtaa cgtttaccat
      121 tttccagatt gagctgtact ggcgtactgc cctgaacctg tttgatttgc ccactggcga
      181 cctcttcttt catgcgtaag aaatcttgca ccgactgcgt gagatcgaca taaatcgggt
      241 ccagacgttg tacggtaacc agcgaatctg cctgattagc ggtaacgagt gcgccgacgg
      301 tcaccgacga tttcccgctg acgcccgtaa tcggcgaggt gacattcgcg tattgcagat
      361 tgatcgtcgc ctgttcaaca gccgctttgg cgacggtgac attggcttct gcttcattca
      421 gctgggtgcg cgcggtgtcg taatcctgac ggctaacgta gttggtcttc agcaacgatg
      481 cctggcggtt aaaggtgatg cgggcattgc tggcggtaga gagcgctttc gccagcgagc
      541 ctttggcgga gtttagctcg gcctgtaaag gtgcaggatc aatctgatac agcgaatcgc
      601 cctggttcac tttatcgcct tcgataaagt tgcgtttaat gataataccg cccacctggg
      661 gacgtatctc ggcaacttca taaggaacgg ttctaccggg caattcgctc aacacattga
      721 ccgaaccagg ggagagtgtg acgacaccga cctcaggcgt catggcggcg gcgttttccg
      781 ccgatttgtc atcgcaggcg gtgagcatcg cgccgcagaa taacaacggt attaacagct
      841 ttcttcttct gttcatttta gtccctgaaa attcttgaga aataacggtt ctttaaattg
      901 cgcgcgagga gagatatgag ggatttacag accagcggca tagtggggtt gtcagccagt
      961 aaggtgggat acaggcacag tgatcgacat ggtgaggtca acgacaaaaa aaatgttgcc
     1021 gttctgccaa cagttcctgc cagcattcgg gcaacgaagg gttctactgg tggatacaca
     1081 taccagggga atcaagtcgt tttagcctct cctttagtaa tcaccggggg taatgaaatc
     1141 agcatctgca tccctcgtca tgccagccat caatttcagt tgcttatgtc ctgactaaaa
     1201 ataagatgtg atacccaggg tgacgatgtc gctcatacgt cttagtttca gcttgtacat
     1261 aatgttgaat ttctgaatct ttaatgtttg ttgcgaaata ttgagcgtcg acgtgatatt
     1321 gctttcattt ttatttttca gaatcagttg cataattttg aactgattat gtgaaaaaca
     1381 taattgtgaa ttcttatggg gcaagtgttt accataagta atctcaagaa gatttttgat
     1441 actttctttg cggctaacaa aataaatagg cgctttagct tttagtttac tgatgtgata
     1501 aggtgccaaa acaataattc tctcggcatc taatttctcc agaaatttaa tcgcttcttc
     1561 atcaaggata tgattgtgat aggtatcaat gataaccttg aagggtttat tgcctacttt
     1621 tttgatctct gacagggaat tgatttttat catttcgtga ttatctttca actgccaaaa
     1681 gccctgtaaa agaaaagaat ctttcgtcat gagaaaaatc ataacttgct ccttagccgt
     1741 tatcgtttcg aacatgtcat ccttttcatt atttacatcc ttgtccgaat cgttgttcaa
     1801 tatagtaaac gcctataaga actttgccct tcgtgaacta atacgtctat taacactaat
     1861 caatacttca gatttatcca tcacgcactc catatattga tcctaagtaa gattcctggt
     1921 tgttatcagc ttgtaaatag taaacaaaaa gttaacgttt gcggattact gcccaagaat
     1981 aagttatcaa taaccagttt gtgatctctg aagaatatta ctaaagttaa aatgtaatcc
     2041 gatttaaata tcgagtctcc ttgtttcgac ttaagctggc aattggattg ccagctttct
     2101 tttaacgcat tgaaattacc tgagtaaata acgcattcat gtcttcaggt ttttatatag
     2161 cgtctggaga cacggatatt tatgcaatgt aatcaacgcc agttgcaaaa aaatgaaccc
     2221 ggattactta tttaaccgcc catagcatca acgcttacgg aactctttgt tcactttccc
     2281 acagcacttt taaaacatta aacctgacta cggaaaatat cagccatgat aatgtttgaa
     2341 atggctaatt tgccatggag tgaaaaaaat tagatgaaat tcagtaggtt gaaataatca
     2401 ctagcaggta attatttcaa tgatagtgcg caattgatct acaacactgc gtagcggaga
     2461 gagtattaat cggatcatag tcacatcaag tgactatgat ccgggtgaca accggggtaa
     2521 ttattgctgc ttaacgaaca aactggcgaa gctgaacagg ctggcggcgc taaatatcag
     2581 ttcaattccc accagtgtgg aaaccagcgt tacagacacc atcggcgttg caccaaggaa
     2641 tatccaggca atgacgatat ccagcacacc aataacgagc tgtagccagc tgcctttcat
     2701 tgaacgctga cgataccaac tcatcaggcg aataacccct gcaacacaga acaaaccggc
     2761 aataaatgcc gcaatggcaa aaatgcccag ctccggtgca cggatgaaga aatagccgat
     2821 caataaatag gcgactgcga cgaggaaacc ggataatacc ggccagaaat tatgactgcg
     2881 gttgctgaat aacccgacaa taagcgcaat acccgagcag attaataatg cacccactac
     2941 tgtgcttaaa atatcgccag agacgaacgg gaaactgata cacagcaacc cgacgataaa
     3001 cagcagcacg gcaataaact ggattgctct gcgatgcttt ttcagcatct ccagatcaaa
     3061 cttcaaaatt gttgccttat ctatatataa catagaacca ccctataaaa ttaagaagaa
     3121 aatcccctgc tatcaatcta tgccaaaaac gcgtctaaga atgcagtcga tttaataaaa
     3181 atttcctaat tgcagtatct gatgcatctg taactcattg tattgaaata aaaatatctg
     3241 attttgatat tttccatcaa catgacatat acagaaaacc aggttataac ctcagtgtcg
     3301 aaattgattc gtgacggctc tttcacttta tagttgagga tattacgatg aaaaaagtat
     3361 taggcgttat tcttggtggt ctgcttcttc tgccagttgt gagcaatgca gcggatgcgc
     3421 aaaaagcagc tgataacaaa aaaccggtca actcatggac ctgtgaagat ttcctggctg
     3481 tggacgaatc cttccagcca actgcagttg gttttgctga agcgctgaac aacaaagata
     3541 aaccagaaga tgcggtttta gatgttcagg gtattgcaac cgtaacccca gctatcgttc
     3601 aggcttgtac tcaggataaa caagccaact ttaaagataa agttaaaggc gaatgggaca
     3661 aaattaagaa agatatgtaa ttccgggaat gcgttacatc gtacttcctt gcatattgaa
     3721 caggccggaa tatcttcttt aaaagcagct attcctcctg ttcatatata atctctatat
     3781 tgaatgggtt acaaaatgaa tatttcatct ctccgtaaag cgtttatttt tatgggcgct
     3841 gtagcggctt tgtcactggt gaacgcacaa tctgcgttgg cagccaatga atctgctaaa
     3901 gatatgacct gccaggaatt tattgatctg aatccaaaag caatgacccc ggttgcatgg
     3961 tggatgctgc atgaagaaac agtatataaa ggtggcgata ccgttacttt aaatgaaacc
     4021 gatctcactc aaattcctaa agtgatcgaa tactgtaaga aaaacccgca gaaaaatttg
     4081 tataccttca aaaatcaagc atctaatgac ttgccgaatt aatgaggtac aagtaaaaag
     4141 gagtagcaag ttgagccatc ttgctgctcc tttttgcatt tttatatgac agcagaattt
     4201 attatacgtc ttatactcgc ggcaattgcc tgtggcgcta ttggcatgga aaggcaaatg
     4261 cgcggcaaag gagcagggtt acgcacacat gtattaattg gcatgggaag cgccctgttt
     4321 atgattgttt cgaaatatgg ttttgctgac gtgctgtctt tagatcacgt cggactcgac
     4381 cccagccgta ttgctgctca ggtggtgacg ggcgtcgggt ttatcggtgc aggtaacatt
     4441 ctcgttcgta accaaaatat tgtaggtctg acgacggcag cggatatctg ggtgaccgcc
     4501 gcataggtat ggttattggc agcggtatgt acgaactggg tatttatggt tcagtgatga
     4561 ccttgttggt gctggaagtc ttccatcaat taaccttccg cctgatgaac aaaaattacc
     4621 atttacagct gacattagtg aacggtaata cggtatcaat gctcgactgg tttaagcagc
     4681 aaaaaataaa aactgattta gtttcgttgc aggaaaatga agatcatgaa gtggtcgcca
     4741 ttgatatcca gcttcatgcc acaacgttga tagaagacct gttgcgatta ttaaaaggta
     4801 tggcaggggt gaaaggcgtt tcaattagct aatctgtgtg gcagcgtagc cagactcacc
     4861 gtaagcctga aattcacacc agataatcaa tatgctgatg gcgtaccagc tcattgatcc
     4921 gcttaagatt gaggatcatc atgatattgt gtttatggca gtagaacgtt tttagcgata
     4981 ttttcagttt ctccagaata tcttcagttg tcatttgatt cgcaatgcag ctcagaacct
     5041 gatgttgctt gcgcgaaagt gacacattcg cttcaggcac agagcagtaa ctgccattga
     5101 tgagtgcgac aaaatcaatg atagtgatag tccgcggaac aaaggtcacc ggtactttcc
     5161 ccagacagcg tttaatatta aagggggaga aaacaatgac atggacaggt tttaatcgtt
     5221 caatttgagt gagaaaatta gaatgaaata cattattcat taatgtatca ataatgacga
     5281 aggcattctg atgcaacatt acgtcgatct cttcttcgtt ctgtatatga acgacattac
     5341 ctttactcag aatgtttttc atcgcggtga agaacatcgt atccctggta attataagaa
     5401 acataaccga ctcctggtgc ggacattaac tttataatca gtttgccaaa ggtaattaac
     5461 aggacagggc ttataataat aatagttagc ttactgtata ttaattttat gtaatacatg
     5521 atgcagatgc tgagaaagca tctgcatctt tcggtggtgt tatttgacca gctcaggtgt
     5581 tacctgactc accgcattgg tgtagtaagg cgcaccccag cccggttccg gccagaatgc
     5641 accatagccg taatcccaca ggttataggt ggtattaacc acttctctca aatgccacac
     5701 ctggatgccc tgcatattca cttccaggaa gttatacggg actttattga taaagcctgg
     5761 ttgttcaccc tgaatggtac cgaggatggt aacaaagtga ttacgatagt tcactggatc
     5821 aaggaagccg ctttgtctgg cgagcagtcg gccctgatag ttggcttcaa tatcaggctt
     5881 cgcatagcta tccaacggta atacagcgat ttctaacaac gtatccgttt tgccattgat
     5941 aacgttgata accttcccac caaagcgcgc ttgttgacca acatataacc ccggctggtt
     6001 atgaacagca acaaaacttt tttgaatatc aggttggtta ttgcctttga tattttgcgg
     6061 aattgaacta catgcggcaa gcaaaaatga aaggctgagg atgagtgcac cttttgtcat
     6121 gttcatgtta ctatccttat caacaaatat taaaaccata atagatattt ctttatagtt
     6181 ttcatctgat tctgaggtga tatgcctgat agtaacgtaa acctgtctga tgtttatatc
     6241 ccttctgttt agttcaattc cgttattttg gggcgtggta tgagtatcca tcgcccaata
     6301 atataaccct ttgttttaaa attacataaa atctattttt attcaatgat tataaagtgc
     6361 taataatcaa tattatggta ttatattctg ataatgattt gataagcgaa ttcttttgcc
     6421 agatggtatt tctatttcca ccccataatt tatatctttt tcattaggca gatctctctt
     6481 taaataaaag gggtatgaaa tcttttcggg ttgaagtgtc tcgtaatgaa attctatgaa
     6541 ttcattttta gacattgttg cccatttttg aattttattg tatagagagg gtgttgtaaa
     6601 ctcttgaagt tgtttataac atattttttt gaactcttgc ttatatttta atatgtcatc
     6661 aaatgaaaga ttcactacct ttgcctctaa gaaatcaatt attctcgaag caatttccgc
     6721 tttcgcgcgt aaagagttgt cattaatagt taacaatgta gaaaacaaac cattgatttg
     6781 tttgtcaaga tgttgttcat atttatgtag tttggggatg ggattttcct tgtgtgaaaa
     6841 aatagtgtta aataaatttg cgtcagtgaa atttatatta agtagatctt caaaataaaa
     6901 ttttgataag gttaggacta gcttggacat atcttgccct gtcatgttga aatcaataaa
     6961 gtcgcatcca gcaagggctt ccatcctatt ttcattttta ctgtgctcaa tatacagctt
     7021 cgccattttt aagtacaagc tttgaatgtc tcttatatca gttggtaagt cagctcgttg
     7081 cccagcaaaa tttttattat cggaagaaac ggtttggaca ctgaatcttt cgggtattcc
     7141 ttcttctacg agaattttag ttatgattgg attgttgggt tctggattaa cgacaacaat
     7201 gatttgacat acagaatcct tatccttttc gtatctaatg ttatgaatct gattaagtat
     7261 tgattgtgtc tgttgcgtaa gtggtgtcga acttatcgtt tcaatgttat taataagact
     7321 tacaagttga accacaactt ttgagtagtc tgcatttgta tatgtggtgt tcgtgtggca
     7381 tgcattaatt tgttggagtt gttgttgcgc taaatcaaat tgttcagggt tgtatccttc
     7441 tccagagaat gtggaattaa tgggttgttg ttcttcccaa ttatcacaag gccacttcgg
     7501 tgtgtctggc gttttaacat tatcggtatt tgttgtgcag ttataaatat cgccgtgata
     7561 tgtatttatt atacctgggg taaagtcaat tgacataaca ttccttattc atcatctgtt
     7621 ttatgctaat gtttttaaat tggataatta gaacaggaat ggattttctt ctacacacaa
     7681 taaatataat gcgttatatc ttcctgatat aattaccttt aaattccctt tcatttccac
     7741 ccggtaatat ttgtgtataa aaaaagggca gtgatagtat cgtaaaggaa aacgttaaca
     7801 actatcaaaa aacgctcatt ttaaaaagtt attatgttct ccgatagtgt gtgaagtaat
     7861 atttttgtga tgttttagat gtgtaataat gaaccaggtt aaagtgtatt taaaaatgtg
     7921 ttgtattagc gactgaaatc tgccttttaa aaaggaggag ggtactgaga caaagagtca
     7981 ttgcttctcc gataaaatgt cagagaagca tgattacgtg gttattgtct gttgatgact
     8041 tgttgaccga gccgtcatca ttctcaatgc attagttaaa tcatttgtga tttttaagga
     8101 ttatcctgtc tatgattttc tacttagaag gatatggcac taactatgca tcttatagtt
     8161 tactgctggc atctatttaa aatagcaatt gagtcaaata gcccatcaag atagccatac
     8221 ctaatataac cgtcaggaat gcagctatca tcggcattct gaacattgat ttaagtaata
     8281 tcacttccgt caggcttgcg ccggcactgc cgatgattaa tgccattaat gctcccagac
     8341 ccattccttt tgtcatcaga acagatgcca gagggataac cgcctcagcc cggatataca
     8401 gcggaatacc gacaacggcg cttaatggga tggcgaaggg attatctgct cctgcatgag
     8461 cggcaatcca cgctgaggga ataaagccat aaataaaaga tcctattaat actcctaaca
     8521 aaagatatgg cagtacatct ttaaactgtt gcaacgcatc tttggtggct agttttatgg
     8581 gattaatact tactacagct ttggtactgc aacaggtaac tacggggttc tcaatggctt
     8641 tagccgctgg actacaacaa ctcactttta tgggcgtata tgtcgttccc ggcgaagttt
     8701 ttgctggagc acaacaattt gctgatgagc ttttactggc aatgatatga cgttcaaacc
     8761 ccagagaatc caggataata ctggcaagaa cggagacgcc ggctgcgata atcgcgtaca
     8821 acaaggtgac tttccaacca aaggtcatcc acattagtcc gacgataatg ggattaagta
     8881 atggtgaaac aaataaaaaa gtgagagtcg gaccaaaccc ggctttcgct gacaacaatc
     8941 cacgtaacat gggtattgtc gagcaactac agaacggggt aacggctccc aacagagcag
     9001 ccaggagata gcctctccct tttctggccc ccatcatctg ctggatttta tggtctggca
     9061 ccttttgtct tatcaggctg acaccggcac taatcactat aaacagcaac gaaagctcga
     9121 cggcgagaaa cacaaacatc tctg
//
LOCUS       DAJTQQ010000135         9019 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000135 DAJTQQ010000000
VERSION     DAJTQQ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.135"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(87..1463)
                     /gene="trkA"
                     /locus_tag="OO309_003631"
     CDS             complement(87..1463)
                     /gene="trkA"
                     /locus_tag="OO309_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR5848631.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1485..2774)
                     /gene="rsmB"
                     /locus_tag="OO309_003632"
     CDS             complement(1485..2774)
                     /gene="rsmB"
                     /locus_tag="OO309_003632"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR5848632.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2820..3767)
                     /gene="fmt"
                     /locus_tag="OO309_003633"
     CDS             complement(2820..3767)
                     /gene="fmt"
                     /locus_tag="OO309_003633"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR5848633.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3782..4291)
                     /gene="def"
                     /locus_tag="OO309_003634"
     CDS             complement(3782..4291)
                     /gene="def"
                     /locus_tag="OO309_003634"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR5848634.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4456..5580
                     /gene="dprA"
                     /locus_tag="OO309_003635"
     CDS             4456..5580
                     /gene="dprA"
                     /locus_tag="OO309_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR5848635.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5552..6025
                     /locus_tag="OO309_003636"
     CDS             5552..6025
                     /locus_tag="OO309_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR5848636.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6054..6596
                     /locus_tag="OO309_003637"
     CDS             6054..6596
                     /locus_tag="OO309_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCR5848637.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6601..7173
                     /gene="tsaC"
                     /locus_tag="OO309_003638"
     CDS             6601..7173
                     /gene="tsaC"
                     /locus_tag="OO309_003638"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR5848638.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7178..7996
                     /gene="aroE"
                     /locus_tag="OO309_003639"
     CDS             7178..7996
                     /gene="aroE"
                     /locus_tag="OO309_003639"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR5848639.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7993..8250
                     /locus_tag="OO309_003640"
     CDS             7993..8250
                     /locus_tag="OO309_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR5848640.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8226..8780)
                     /locus_tag="OO309_003641"
     CDS             complement(8226..8780)
                     /locus_tag="OO309_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR5848641.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2254 a         2190 c         2251 g         2324 t
ORIGIN      
        1 ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa agggaaataa
       61 taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg gaagagtctt
      121 tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc gccttgctca
      181 atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc tccaataatc
      241 gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga ggtgctttca
      301 tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga ggaaacacca
      361 acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg ttgtggtgaa
      421 attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg ctggatcaat
      481 accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat attggcctcg
      541 tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc ggccagcagt
      601 tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc cgccagttcg
      661 gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc cagacgacgc
      721 gccagccctg caccgatatt accgccacca accagcatga tccgtttata cggtttttcc
      781 agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat aaagaacact
      841 tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc gtggcggaaa
      901 attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga aagtgcatta
      961 ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt accctcagcg
     1021 aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt atcgataacc
     1081 aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa tagcttatcg
     1141 gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt gttgaaaagt
     1201 gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac agcaaccagc
     1261 atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc atgcccctgc
     1321 acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt ggtatcgaca
     1381 acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc aacctggccg
     1441 gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt tttgattagc
     1501 ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt acccggttgc
     1561 tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa ggctttaatc
     1621 tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt tccaccggat
     1681 tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag ttccgggata
     1741 tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc cgttgctgaa
     1801 caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg ggaagggtaa
     1861 cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt gtcgtaaatg
     1921 cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg tgccacctca
     1981 aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg ttcaccgttt
     2041 tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt aacccatccg
     2101 tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac agcatcaggg
     2161 taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag ccagctgtcg
     2221 cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt attggcttcg
     2281 gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa ccaggaagga
     2341 tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg ccgacgctgg
     2401 aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat tgcgatagcg
     2461 ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag cagttgatac
     2521 aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg acgggccatt
     2581 aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa gcacaactct
     2641 tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag aatgttgctt
     2701 aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct acgtaaatta
     2761 cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag agtggactat
     2821 cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag gtcttgcgcg
     2881 ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc atcaccagtc
     2941 gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc gttggttgcc
     3001 gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc cagccagctc
     3061 attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga aagtgaccag
     3121 tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt ttcgtcctga
     3181 acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag cccttgtggg
     3241 ccaagctctg ccagcttgtc gtacaacgta ccactggtat cttctgcagt aatcgggcag
     3301 gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat aatggttaca
     3361 ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc gcgccagcgt
     3421 ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag cacagcttta
     3481 ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc ggcgaccagt
     3541 tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc ttcttcctca
     3601 gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg tcggtctggc
     3661 tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag atgacgcgct
     3721 gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt agttcttatc
     3781 cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt cgttgttgtt
     3841 tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc tcatgctgaa
     3901 tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg cggtcaaggg
     3961 cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg atcgacaggc
     4021 aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg attaacacca
     4081 gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg atatcaacct
     4141 gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata tcatcgacga
     4201 tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga agccgctcgt
     4261 ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt caggagttag
     4321 aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc acttgattga
     4381 cagcatcact tgattgacag catcactgac caatcgcaaa gattgctaag gcttgcttat
     4441 ggcagggaga taaggatggt cgatatagaa atttggctgc gtttaatgag catcagcagc
     4501 ttgtacggcg atgatatggt ccgtatcgct cactggctgg caaaacagtc gcatattgat
     4561 gcggttgtat tgcagcaaac agggcttaca ttgcggcagg cacaacgctt tctttcattt
     4621 ccacgaaaga gtatcgaaag ctcactttgt tggttggagc aacccaacca tcatttaatc
     4681 cctgcggaca gcgaatttta tcctcctcaa cttcaggcga cgacagatta ccccggcgca
     4741 ctgtttgttg aaggagaact gcacgcgctg cattcatttc agcttgccgt agtggggagt
     4801 cgggcgcatt catggtatgg cgagcgatgg gggcgattat tttgcgaaac tctggcgacg
     4861 tgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggt gcataaagcg
     4921 gccttacagg taaatggcgt cagcattgct gtattgggga atggacttaa taccattcat
     4981 ccgcgtcgcc atgcccgact ggctgccagt ctgtttgaac agggtggcgc tctcgtctcg
     5041 gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa tcgcattatc
     5101 agtggtctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg ttcgctggtg
     5161 acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg tccaataggg
     5221 aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct tgtgacggaa
     5281 ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga cgcccctgaa
     5341 aattcatttt attcaccaga tcagcaagac gtggcattgc catttcctga gctcctggct
     5401 aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg ccaacctgtg
     5461 ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc agctgtaccc
     5521 ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt atttgtttga
     5581 aacctatatt cacactgaag ctgagttgcg tgtggatcaa gacaaacttg aacaggatct
     5641 taccgacgca ggttttgagc gagaagatat ctacaatgcc ctgctatggc tggaaaaact
     5701 tgctgattat caggaagggt tggcagaacc gatgcaactg gcctcggatc ctctctccat
     5761 gcgtatttat acaccggaag agtgtgaacg actggatgcc agctgccgtg ggtttctgct
     5821 tttccttgag cagattcagg tgctcaacct tgaaactcgt gaaatggtga tagagcgagt
     5881 gctcgcgctg gataacgcag agttcgaact ggatgatctg aaatgggtga tcctgatggt
     5941 gttgttcaac atcccgggct gcgaaaatgc gtaccagcaa atggaagaat tactctttga
     6001 agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagtt gttatggcaa
     6061 aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg gctgaactgg
     6121 ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg gcgtgtgact
     6181 acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg gaggggcagg
     6241 tttgccccgt atgtggcgca aatctggtat tacgccaggg acgctttggt atgtttattg
     6301 gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg gacgaaacag
     6361 caattacatg cccccaatgt cggacgggcc atctggtcca gcgccgctcc cgttatggca
     6421 aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac ttcaaaccaa
     6481 tagctggaga atgccctgag tgtcattatc cgctactcat cgaaaagaaa accgcgcagg
     6541 gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg gaataataac
     6601 gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct caatgaaaaa
     6661 cgtgtcatcg cctatccaac ggaagccgtt ttcggtgtcg ggtgcgatcc tgatagcgaa
     6721 acagcagtga tgagactgtt ggagttaaaa cagcgtccgg ttgataaggg gctgatttta
     6781 atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgtt gactgacgtg
     6841 cagcgtgaaa ccattttttc ctgctggcca ggtcctgtca cctttgtctt tcccgcgcct
     6901 gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg agtcaccgac
     6961 catccgttgg tggttgcttt gtgccaggct tatggtaaac cgctggtttc taccagtgcc
     7021 aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca atttggcgcg
     7081 gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga aatccgcgat
     7141 gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg ctgtttttgg
     7201 taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc agcaactgaa
     7261 tattgaacat ccctatgggc gcgtgttggc acccatcaat gatttcatca acacactgaa
     7321 cgctttcttt cgtgctggtg gtaaaggtgc gaatgtgacg gtgcctttta aagaagaggc
     7381 ttttgccaga gcggatgagc ttactgaacg ggcagcgttg gctggtgcag ttaataccct
     7441 caagcggtta gaagatggac gcctgctggg tgacaatacc gatggtgtag gcttgttaag
     7501 cgatctggaa cgtctgtctt ttatccgccc tggtttacgt attctgctta tcggcgctgg
     7561 tggagcatct cgcggcgtac tactgccact tctttccctg gactgtgcgg tgacaatcac
     7621 taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca ctggcagtat
     7681 tcaggcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta ttaatgcaac
     7741 atccagtggc atcagtggtg atattccggc gatcccgtca tcgctcattc atccaggcat
     7801 ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat ggtgtgagca
     7861 gcgaggctca aagcgtaatg ctgatggttt aggaatgctg gtggcacagg cggctcatgc
     7921 ctttcttctc tggcacggtg ttctgcctga cgtagaacca gttataaagc aattgcagga
     7981 ggaattgtcc gcgtgaatca ggccatccag tttccggaca gggaagagtg ggacgagaat
     8041 aaaaaatgtg tatgttttcc cgctctcgtg aatggtatgc aactgacatg cgcgatctct
     8101 ggcgagagtc tggcgtatcg ctttactgga gatacgccag aacagtggtt agcgagtttt
     8161 cgtcagcatc gctgggacct ggaagaagaa gcggaaaact taattcagga acaaagtgaa
     8221 gatgatcaag gctgggtctg gttaccctga tccagatatt cgtccttcca tttcacgtaa
     8281 ttattcgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg gatctgtttg
     8341 acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg gaccagacta
     8401 cccgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat tgagcccatc
     8461 ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg accaacagtg
     8521 acatcttcgc caatggttaa tgggttgcca tctgggttgt acgaggattt atgagtgaca
     8581 tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata atgtacatct
     8641 ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac gtcaccaatc
     8701 acgacactgc tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag atcgcggtat
     8761 gggcgtaaaa catcagacat acttacctca gcaataaatg atttactaat gactttgggg
     8821 gcattattgg ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt gtgtgcgatt
     8881 gcagcaaaaa gggtgaaaaa acaacaaaca gaaaagaaga tcaaaaaaat acttgtgcaa
     8941 aatattggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca cttcacagga
     9001 tggtcggaac aacgaagag
//
LOCUS       DAJTQQ010000136         9011 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000136 DAJTQQ010000000
VERSION     DAJTQQ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9011
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.136"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            35..691
                     /gene="pdxH"
                     /locus_tag="OO309_003642"
     CDS             35..691
                     /gene="pdxH"
                     /locus_tag="OO309_003642"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR5848642.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            820..2094
                     /gene="tyrS"
                     /locus_tag="OO309_003643"
     CDS             820..2094
                     /gene="tyrS"
                     /locus_tag="OO309_003643"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR5848643.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2153..3016
                     /gene="pdxY"
                     /locus_tag="OO309_003644"
     CDS             2153..3016
                     /gene="pdxY"
                     /locus_tag="OO309_003644"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR5848644.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3060..3665)
                     /gene="gstA"
                     /locus_tag="OO309_003645"
     CDS             complement(3060..3665)
                     /gene="gstA"
                     /locus_tag="OO309_003645"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR5848645.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(3771..5273)
                     /gene="dtpA"
                     /locus_tag="OO309_003646"
     CDS             complement(3771..5273)
                     /gene="dtpA"
                     /locus_tag="OO309_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR5848646.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(5884..6519)
                     /gene="nth"
                     /locus_tag="OO309_003647"
     CDS             complement(5884..6519)
                     /gene="nth"
                     /locus_tag="OO309_003647"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR5848647.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6519..7214)
                     /gene="rsxE"
                     /locus_tag="OO309_003648"
     CDS             complement(6519..7214)
                     /gene="rsxE"
                     /locus_tag="OO309_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR5848648.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7218..7838)
                     /gene="rsxG"
                     /locus_tag="OO309_003649"
     CDS             complement(7218..7838)
                     /gene="rsxG"
                     /locus_tag="OO309_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR5848649.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(7842..8900)
                     /gene="rsxD"
                     /locus_tag="OO309_003650"
     CDS             complement(7842..8900)
                     /gene="rsxD"
                     /locus_tag="OO309_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR5848650.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         2306 a         2260 c         2288 g         2157 t
ORIGIN      
        1 tagcgtcacc cactgacaat ccgtaaagaa aaccatgtct gataacgacg aattgcagca
       61 aatcgcgcat ctgcgccgtg aatacaccaa aggcgggtta cgccgccgcg atcttcccgc
      121 cgatccatta accctttttg aacgttggct ctctcaggct tgtgaagcca aactggcgga
      181 ccctaccgcg atggtggtcg ctaccgtgga tgaacatggt cagccttatc agcgcatcgt
      241 tttactcaaa cattacgacg aaaaaggcat ggtgttttac accaacctcg gtagccgtaa
      301 agcacatcaa attgaaaata atccgcgcgt tagcctgctg ttcccgtggc atacccttga
      361 acgccaggtg atggtgatcg gtaaagcaga acgactttcg actctcgaag tgatgaaata
      421 ttttcatagc cgcccgcgtg atagccagat tggtgcatgg gtttcgaagc agtccagtcg
      481 catttctgcc cgcggtatcc ttgaaagtaa attcctggag ctgaagcaga agtttcaaca
      541 gggcgaagtg ccattgccga gcttttgggg cggttttcgc gtcagccttg aacagattga
      601 gttctggcag ggcggtgagc atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga
      661 tgcgtggaag attgatcgtc ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct
      721 ggtactcctg attctggcac tttattctat gtctctttcg catctggcga aaagtcgtgt
      781 accggcaaag gtgcagtcgt tatatacatg gagattttga tggcaagcag taacttgatt
      841 aaacaattgc aagagcgggg gctggtagcc caggtgacgg acgaggaagc gttagcagag
      901 cgactggcgc aaggcccgat cgcgctctat tgcggcttcg atcctaccgc tgacagcttg
      961 catttggggc atcttgttcc attgttatgc ctgaaacgct tccagcaggc gggccacaag
     1021 ccggttgcgc tggtaggcgg cgcgacgggt ctgattggcg acccgagctt caaagctgcc
     1081 gagcgtaagc tgaacaccga agaaactgtt caggagtggg tggacaaaat ccgtaagcag
     1141 gttgccccgt tcctcgattt cgactgtgga gaaaactctg ctatcgcggc gaacaactat
     1201 gactggttcg gcaatatgaa tgtgctgacc ttcctgcgcg atattggcaa acacttctcc
     1261 gttaaccaga tgatcaacaa agaagcggtt aagcagcgtc tcaaccgtga agatcagggg
     1321 atttcgttca ctgagttttc ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac
     1381 aaacagtacg atgtggtgct gcaaattggt ggttctgacc agtggggtaa catcacttct
     1441 ggtatcgacc tgacccgtcg tctgcatcag aatcaggtgt ttggcctgac cgttccgctg
     1501 atcaccaaag cagacggcac caaatttggt aaaactgaag gcggcgcagt ctggttggat
     1561 ccgaagaaaa ccagcccgta caaattctac cagttctgga tcaacactgc ggatgccgac
     1621 gtttaccgct tcctgaagtt cttcaccttt atgagcattg aagagatcaa cgccctagaa
     1681 gaagaagata aaaacagcgg taaagcaccg cgcgcccagt atgtactggc ggagcaggtg
     1741 actcgtctgg ttcacggtga agaaggttta caggcggcaa aacgtattac cgaatgcctg
     1801 ttcagcggtt ctttgagtgc gctgagtgaa gcggacttcg aacagctggc gcaggacggc
     1861 gtaccgatgg ttgagatgga aaagggcgca gacctgatgc aggcgctggt cgattctgaa
     1921 ctgcaacctt cccgtggtca ggcgcgtaaa actatcgcct ccaatgccat caccattaac
     1981 ggtgaaaaac agtccgatcc tgaatacttc tttaaagaag aagatcgtct gtttggtcgt
     2041 tttaccttac tgcgtcgcgg caaaaagaat tactgtctga tttgctggaa ataatgcatt
     2101 aagtggaaag ggggagtgag aaatcactcc ccctggtttt tatacaggga acatgatgaa
     2161 aaatattctc gctatccagt ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga
     2221 gtttccgatg cgccgcctgg gcgcgaacgt ctggccgctg aacaccgttc aattttctaa
     2281 tcacacccaa tacggcaaat ggactggctg cgtgatgccg cccagccatt taaccgaaat
     2341 tgtgcaaggc attgccgcca ttgataaatt acacacctgt gatgccgtat taagtggcta
     2401 tctgggatcg gcggagcagg gtgaacatat cctcggtatc gtccgtcagg tgaaagcagc
     2461 gaatccgcag gcgaaatatt tttgcgatcc ggtaatgggt catccggaaa aaggctgtat
     2521 cgttgcaccg ggtgtcgcag agtttcatgt gcggcacggt ttgcctgcca gcgatatcat
     2581 tgcgccgaat ctggttgagc tggaaatact ctgtgagcat gcggtaaata acgtcgaaga
     2641 agcggttctg gcagcgcgcg aactcattga gcaagggcca caaattgtgt tggttaaaca
     2701 cctggcgcga gctggctaca gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga
     2761 agtctggcat atcagccgtc cactggtgga ttttggtatg cgccagccgg taggtgttgg
     2821 tgatgtgacg agcggtttac tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc
     2881 cctggaacat gtgaccgctg cagtctacga aatcatggtg accaccaaag caatgcagga
     2941 atatgagctg caagtggtgg ctgctcagga tcgtattgcc aaaccagaac attacttcag
     3001 cgcaacaaag ctctgaaata tctcatttcg gcccacaacg acctgtgggc cgatggctat
     3061 tactttaagc cttccgctga cagcgcgtct tgtacttccg gacgttcagc catacgttgc
     3121 ataaatgccg caatgtgctc taacccttcc agattcagtt tcaccgcgta tgcccagcgc
     3181 agaaccgtaa acagataggc atcagcaatt gtaaatcttt gcccgcagat ccagtgctca
     3241 tccttcagtg cctcgttcac atattgcagc ttcttatcca gctgcgcgcg aactgtcggt
     3301 ttgtactctt ccggtgtatc tgggcgaaac agaggtgtga aacctttatg cagctcggtg
     3361 gcgatgtaat tcagccattc gatggtttta tagcgggaaa tactgtttac cggtgccagc
     3421 aactggcggt cggggacgct gtcggcaaga tactgcataa tcgcaacgcc ttccgtcagc
     3481 aaagtaccat cgtccagcag caatgcaggc acctgcccct tagggttaac ggaaaagtaa
     3541 tcgtcaccgt tttcgagacg ttttttcatt aaatccacac tgacgagcgt aaaatccttt
     3601 ccgctctcac gcagggtgat atgggaagcg agagagcagg cgcccggttt gtagaacaat
     3661 ttcatcggta actccttttg tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa
     3721 aaaagccgct aacgaaaagt tagcggcttg taaaagagtt tccctgaatt ttacgctaca
     3781 gtagctttcg ccgctttgtc tgcagcgtca tcctgcgtca tgcggtgcag tttaggcgcg
     3841 gtcagcagca tcagtactgc aataacggca gtagcgacac caatctgcaa gaatacgcga
     3901 ccatagactt ccagtgacat cagcgggtcg gtaacgttat ccggcacagc catcataccc
     3961 gcaacataac caccaatcag gtttgcaccg gcagtggtca ggaaccagct acccataatg
     4021 aagcccatca gacgctgcgg aacgagttgt gcaaccattg ccagacccag accagagatc
     4081 atcagttccc cgatgctctg caggccatag cttgcgacca gccagctgac agacacgata
     4141 cctgcgtcag acgcgaattt cgcacccagc ggcagaatca ggaacgcacc agaacacatc
     4201 accatgccga ttgcaaactt ggttggcatc ggcagggtat cacccatctt gttatagata
     4261 gcggccagaa tcggactacc gatgatgatc cagaacgggt tcagtgcctg atactgctca
     4321 ggttctacgg ccagacccag aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt
     4381 gacgttggca tctggctgta cagcacgaag aagatgattg cttcgagcat caggatgaag
     4441 gcaacgatca ttttacgacg cgcagcacct ttcatcgcga aagcttcttt accgaagata
     4501 accacaatac cgaaggcaac aacgcccagc gccatacgcg caacttcctg attgtgcagt
     4561 agccaggtgg caacagcgat cagcgccaca acaccaataa tggtcagcaa caggttacgg
     4621 tagttgactg gttcaaagtc tggttttgaa ccgtactgtt taacccagcg ttggcagaag
     4681 gcgaagttaa cgatagtgat cagcaggcct acaacgctca acgcaaacgc aacactccag
     4741 ccgtatttcg cggccagcca cggggtggct atcatagaga agaaagagcc gatgttgacg
     4801 gacatgtagt acatggtgaa tgcaccgtcc agacgtgggt cgtttttctc atagcatgta
     4861 gaaagcagag aagacgggtt agctttaaac aggccgttac cgaccgcaat agccgccata
     4921 cccatataaa caataccggc gtcgtgacca gaccaggcaa ccagagcata accaatcgcc
     4981 agcacaatag cgccgagcat aattacgcgt ttagtaccca gtaccttgtc acctaaccag
     5041 ccgccgatag cgaccagacc ataaaccagg gcactaaagg aagagaaaag ggtgattgaa
     5101 tccgcttcag acatacccag ttgtttaacc aggtaaacag ccataattcc ttgtaggccg
     5161 taataaccaa aacgttccca taactcaatc gagaagatga gatagaacgc cttcggttgt
     5221 ttgaaagcgt tcaaactgac gctttcagtt ggtttttggt ttgcagtgga cacttttacc
     5281 tcttttttta catcccatat taacgggggt gctttctcgc gttatgtcta aaaaacatac
     5341 gcgcaattgt tatagtggga ggggaaacgg caggtaatgt tcactatctt gcactttcag
     5401 acaagtcctt tgtaataatc tgttacatat aactgaggcg gggaattcaa ccattccggg
     5461 aaatgttatc cagcgttaaa tttttcaaaa aacgtaacgg cagatttttt cactgcattg
     5521 agagggggat gatggcttgg cggcgatatg ttctgctatt tagcgttttt cctagtgaga
     5581 tagtccagtt tctgaaaaat agccagtgta atgttttgca ggtcaatatt tgttgcgtgt
     5641 gtaaatttaa atcattttaa cctgattctt gcaatgtttt tacattgcca cagcatcgtg
     5701 atcttgatca cgaatgaata gaaaatttcg ctattaaaaa aacaattaac ctgcattttt
     5761 ggttatccgc aaacggatta cctgatgata acgctaagca atggcatcat tgacgttaaa
     5821 aagatgtttt aaaagaggat aaagaaaggt tatcaatggg gtaatcggtg ttaccccttt
     5881 tcttcagatg tcaactttct ctttgtattc acaaagatct tcaataatac aagagccaca
     5941 gcggggcttg cgggcaatgc aggtataacg cccgtgcagg atcaaccaat ggtggcagtc
     6001 gactttaaac tctgccggaa ccactttcag tagcttttct tctacctgtt cgacgttttt
     6061 ccccggcgca aattgagtac gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt
     6121 cggccagccg aatgcagtgt ttaatacgac gttggctgtt ttacgaccta cgccgggcag
     6181 ggcttcaagc gcagcacgat cttccggaac ctcgccatta tgttgttcca gtaatatacg
     6241 gcaggttttg atgatatttt ctgctttgct gttataaagg ccaatcgttt tgatataggt
     6301 tttcacccct tcaacgccta gttcaagcat cgctgcaggc gtattcgcca ccgggtagag
     6361 tttcgccgtc gccttattaa cactgatatc ggtcgcctga gcggaaagca gtacggcaat
     6421 cagcaattca aaaggcgaac tgaaattaag ctcggtggtg ggatgaggat tgttctcacg
     6481 caggcgagtg aggatctcca gacgttttgc tttgttcatc agacattccc tgtttcaccg
     6541 tttggcaatg cacgttcggc agctgcttca gcacggcgct ttttcattct ttcatcaatc
     6601 aggtattttc ctgccagcat cagtcccaag ccaataaatg cacctggtgg cagcatcgcc
     6661 agcaggaaag gggagtcggt gtggaaaatc tccacgcgta atacttttgc ccagctacct
     6721 aacagcgcat ctgcaccgtc aaacaacgtg ccattgccga taatttcgcg tagtgaaccc
     6781 agcacgaaca tggcgcaggt tgcgcccgta ccaattgaaa agccgtccag tgccgaaagc
     6841 gccggacctt ttttggcggc gaaggcttca gcgcggccca caacgataca gttagtgaca
     6901 atcagcggaa taaaaatccc taatgattga tacaggccaa aggcgtaggc gttgatcagc
     6961 atctgtacag cgctgaccac cgaggcgatg atcatcacgt aaatgggaat gcggatctcg
     7021 gctggcgtcc agtgacgcag cgtcgaaatg gtcaggttgg tcagcgtcag taccagcgta
     7081 gtcgcaagtc ctaaacccag agcgttagtg gcagtggatg tgaccgccaa tagcggacaa
     7141 aggccgagca gttggaccag tgcagagttg tttttccaca acccctgaac aataacgtct
     7201 ttaatttcgc tcacgcttta ttctccacag gcaggaagtt gagaaagttg tgccggtaac
     7261 gtctgagcgt acaatccggc gcgttttacc gcattaacca ccgcgcgggg agtaatcgtc
     7321 gcgccggtga actggtcgaa atcaccacca tctttcttca ccgcccagtg cgcatcatcc
     7381 gcaccactga ttttttttcc cgcaaaatgg gtgagccagt cagaaaggcg caattcgatt
     7441 ttatcgccaa gccctggcgt ttcgtggtgc tctgtcacgc gcgtgccaag taccgtgcca
     7501 ttaaaatcgg ctcccaccag cagctgaatc gcaccggaat agccatctgg cgcggttgct
     7561 tccagaacgg cggctaccgg tttgtcatcc tgtttggcga tgtaaacccg atgctcacct
     7621 ttacctaact ctggcgcagt taccagatag caactctgtg cgagcgcatt gttatagcgt
     7681 tcggctggca gcacctgatc aaataacgtc ttttgttgca gactggcctg ttcagcaatt
     7741 gtcgttttgg tcatctggtt gatggccgca gttaaccctg tagaacccgc tgcaaatagt
     7801 gccagtgtaa tgccgtgttt tcggatagtt ttcagcatgg tttacccttt gcgatggccg
     7861 tagacgcgcg gacgcgtgta gtaatcgatc agaggaaccg tgatgttcgc cagcaggacg
     7921 gcaaaagcca cgccgtcagg atagccgccg aaactgcgga tcaaccagac taataagccc
     7981 gccagcgcgc cgaacatcag ccgaccacga ttggtcgtag aagcagtaac tgggtcagtc
     8041 aaaataaaga atgcgccgag catagtcgca ccagacagca gatgaatttg cggtgctgcc
     8101 agtgtttctg gtgagaacaa ccagcccaac gttgcgcata acgccagcgt tactaagaag
     8161 ctgaggggaa tatgccagcg aatcgctttc tgccatagca accacacgcc accagccagc
     8221 caggcgagat ttacccattg ccaaccagcg cccgccagaa taccgctgta gatcggatat
     8281 tgcataatct gttcaaccga atgacctgcg cggacagagg ttttgaatgt atccagcggt
     8341 gtcgcctgac taatgccatc aatacctaag cgtagcgtat tcatatcacc accactggcg
     8401 gtatgaccgc tgaaaataac ctgtatggcg tcgataaaac cagggatgtt gaccgcaatt
     8461 tcatgtggcg gtaaccagct ggtcatctgc accgggaagg agatcagtaa gaccacataa
     8521 ccaatcattg ccggattaaa cgggttttgc cccagaccgc catacaactg tttagcgatg
     8581 atcaccgcaa acaccgtacc cagtacgacc atccaccatg gcgctagggg gggaatactt
     8641 accgccagca ataagcctgt cagcaatgct gagttatctt tcaacgttgc ggctaccgac
     8701 tgcttgcgta gtttgagtac gagagcttcg gctaacagag cactgaccga cgccaacagg
     8761 atctgaacga gagtacccca accaaaaaac cgcagttgcg ctgcgattcc tggcacggct
     8821 gcgagcaaca ccaacagcat aatgcgcgat gtctggcgct ggttatgggt ataaggggag
     8881 ctagctattc tgaataccat ttagtcctcg tttacaacct tttgctgggc ggcttttttg
     8941 gcctgaacgc gggcaatagc cgcggcaact gcggctttgc gcggatcgac ctgttcttct
     9001 ggttccgcat t
//
LOCUS       DAJTQQ010000137         9002 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000137 DAJTQQ010000000
VERSION     DAJTQQ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9002
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.137"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..192)
                     /locus_tag="OO309_003651"
     CDS             complement(<1..192)
                     /locus_tag="OO309_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HCR5848651.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGPLSVKQGC
                     CVGECGACTVLVDGTAIDSC"
     gene            complement(189..1067)
                     /gene="xdhB"
                     /locus_tag="OO309_003652"
     CDS             complement(189..1067)
                     /gene="xdhB"
                     /locus_tag="OO309_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR5848652.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1078..3375)
                     /gene="xdhA"
                     /locus_tag="OO309_003653"
                     /pseudo
     CDS             complement(1078..3375)
                     /gene="xdhA"
                     /locus_tag="OO309_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            3790..4545
                     /locus_tag="OO309_003654"
     CDS             3790..4545
                     /locus_tag="OO309_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR5848653.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4624..4697
                     /locus_tag="OO309_003655"
     tRNA            4624..4697
                     /locus_tag="OO309_003655"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4656..4658,aa:Gly,seq:ccc)
     gene            4832..6442
                     /locus_tag="OO309_003656"
     CDS             4832..6442
                     /locus_tag="OO309_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5848654.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6474..7081
                     /locus_tag="OO309_003657"
                     /pseudo
     CDS             6474..7081
                     /locus_tag="OO309_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8012..8758
                     /locus_tag="OO309_003658"
     CDS             8012..8758
                     /locus_tag="OO309_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5848655.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
BASE COUNT         2303 a         2208 c         2311 g         2180 t
ORIGIN      
        1 gcaactgtct attgctgtgc cgtcgaccaa caccgtacag gcaccacatt cacccacgca
       61 gcacccttgt ttgacactta gcggtccttg ttcgcggagt aattccgaga gcggcgtgcc
      121 tggtgcggcg tgaagctgaa aaggcatccc gttaatggtg cattcggtgg taattgtttc
      181 gctgtgattc attgcaattt tccccccgcc gcggcgacgg cttcgctaat cacttttttg
      241 gtcatcgtct ggatgagatg cagacgaaac tctttactgg cccgccatga agaacgcggg
      301 gcgacatctt gcaggacaga ttcgctgata gcttccagcg tttgcaggtt taatggcgca
      361 ttttgtgcag tctgttcggc atgttggcag cgaatcggcg ttggcgcggc aacaccaaat
      421 gccaggcgta attcgctgaa attgccgtta tccagtcggc aatgtgcggc gcagccaatc
      481 gttgaaatat ccattgcgtc gcgcatggca tatttaaaat gcgcgctgcc cgcgtgttct
      541 ttcggctgtg gcggaaaatg aaaggcgacg aggatttcgt catgctcaag agacactttg
      601 cccggcccgg tgtgaaagcc attaatcggg acgaaacgaa caccgcgcgg ggagtggatc
      661 tccagtttcg cgtcataaat tagcgttggc gtggcagaat ctgcgctggt ggcaccgttg
      721 caaatatttc caccgtaggt agcgacgtta cggatctgcg gtccagcaat gaacgtggcc
      781 gcagcacata acgccgggag atgacgttga gttataggat cttctattag ctgggtaaat
      841 gtcgttgcag agccgatacg tagcgagcca tcttccgcca gcgtaattcc ccgcagctcc
      901 gccagattat gaatatcaac aatatggcga taacggtcat tgtgatggtg gagctgaatc
      961 agtacgtcag tgccaccggc gagcagtttg gcctgcgggt tgtcagccag caggttgatg
     1021 gcatcggcaa gggttgctgc gcgatggtaa gaagcaaaat caaacatgat gttatcctca
     1081 aatcaatcct gccagatgga actcttcata taaccgtttt ggcgtcagcg gcagtgtatt
     1141 gattgcaaca ccggtagcca tcttcaccgc gttacgaata gcagcggcaa caggaattat
     1201 gggtggctca cccagtgact tatgtccgta tgcggattgc ggctcattga tttcgacgaa
     1261 cgcgctttcc agttgtggca gatccggcat ggtcggcatt ttgtaatcca gcagattggg
     1321 gttacggacc acgccgcttt tagcatcgat gatcatctct tcaaatagcg cccagccaat
     1381 gcccattccc attccgccgt gtacctgacc ttctgccagc agtggattaa gaatatgccc
     1441 tgaatcatga acgttgagga tgcggttgat ggtgactttg cacagcgcaa tatcgaccgt
     1501 cagatcaaca aaggtacagc caaacgccgg tgggttagtg gtggttttga tggagctttc
     1561 agcagagagc tgcccgccgc gttcagggtg gtagaaagcg tccatcgcca aatcttttaa
     1621 cgacattaac ggctcttccg gtcgttcaac cagcacgata tggcctttta tcagggtcaa
     1681 attcatcgct gactgatgta gcatgactgc ggcgtgagcg atgattttct cttttaataa
     1741 tagtgccgca ctgcgcagcg caggcgcggc aacatagctc tggcgtgagg caaatgcgcc
     1801 gggatcgaac ggcgtaacgt cggtatcttg tgttgaaata acgcgaacgt cgctgaccgg
     1861 aacccccacg gtttctgcca ccatttgcga gaagacggtg tcggcaccct gaccgatttc
     1921 cgtcgcgccg ctttgcacgt tgatggttcc atcctgattc atcagaaggc gcgcgcctgc
     1981 tatttctacg ccgacaggcc aggtgttaga ggtgtagcta aaacaggcga cgccaacgcc
     2041 gcggcgcaaa ttgccttgct ggttctggca ttctgcacgg cgtttttccc attcaaagat
     2101 tttccggcct ttttcaagac actccggcaa ccctgcgctg caaatacgtt tgcccgtgag
     2161 cggattagca tctccttcgc gggcggcgtt gcgtaaacga atttcaacag gatcaatacc
     2221 taacgctgtc gcggcgtcat caagcataga ctcaacggca aatacgactt gtggcgcgcc
     2281 ataaccacgc atcgcaccag ccgaggggag gttggtatag caggtctttg aactgtaagc
     2341 gtaggcacaa cgaggataaa ggtaagcgac tttattcccc cccgcagaag cgatggagtg
     2401 cccgtgagat gcataagcgc cggtgttaga cagaacatcc agactataac ctttcaaggt
     2461 tccgtcgcgg ttcacgccca tttgcccgtc aatagtaaaa gcgtggcggg tacgggttgc
     2521 gaggaaacac tcttcacggc taagggaaac tttcaccgga atgccgccaa gcttgctggt
     2581 caggaatgcc gccattggct cttccagtac atcctgttta ttaccaaaac cgccaccgac
     2641 aaacggtttg atgactcgta cgcatgacca gggaatatcc agcgcctgac caaccacgcg
     2701 gcgaacaatg tgcgggatct gggtgctgga aacgatggta attcgcgagt catcctccat
     2761 ccacgccagc gatgttacgc tttccatgtg acaatgttga ataacgggag tctgatagtg
     2821 cccctgtacc tggtagtcgg cggcatcgat tgtttgttgg acattgcccg tcgacatcgt
     2881 gctttgtttc agtaaattgc cgccgttatg gattggtgca gcgtcttctg tcagcgccgc
     2941 ttctggcgtg gtgataacgg gtaattcttg ccactcaatg ctgaccaatt gcgccgcttt
     3001 ttctgccgtg agttcatcgc gggccacgac gatggcaacg gcgtcaccat gatgacgaac
     3061 atggcgagtt agcagtgcgc gatcggcggt atcgcgcttg ttttcgtcaa gtgtctaggc
     3121 atgcccagct gtagcgaatg gaatatcagg cacatcttcc caagtaaaaa tcgccagtac
     3181 gcccggtaaa cttctggctt gttcatcatt aatacttacg gcataaccat gtgcgatagg
     3241 gctacgtaca tatttcgcgt aacacatgcc cgccataaca taatcgtcag tatatcgtgc
     3301 ccgcccggtg accttagcaa tggcatcgac gcgcatgcat gattcacccg tagcggttgc
     3361 ttcccgcgct tccatagaaa tcccctcgat ttgaacattt ggttcttatg gcaaagattt
     3421 agtctaagca ggcaagattt acgccagaaa cgacgataag cgttagggga ggaaggaagt
     3481 gtgatgacgt taaaaaaaca cagatatgat acgaaacagg actctgaata gggacagtga
     3541 tacgtatcaa tatgagtggc gataaaatca atgtgataaa tcaggattat tacgcatcag
     3601 gatgggaaat agggtaaaaa gtcaggaact tcgatacctc tacagcgaat attttgctga
     3661 tgatacttcc ctttgccaaa tgaaattaac ctctttctct attaccacgt ttttccagaa
     3721 gcaagagatt gggtatcaag aggctggctg ctatgatatg gcgtcttgtt ttttaagcga
     3781 ggaaagattt tgagtgcggg acgcctgaat aaaaaatctc tgggtatcgt gatgttttta
     3841 tcggttggac tgctattggc gggctgttcg ggtagcaaat catccgatac aggaacgtat
     3901 tccggctccg tttacaccgt gaaacggggg gatacgctat atcgtatttc gcgcaccacg
     3961 ggaaccagcg taaaagagct ggcgcgactg aacggcattt ccccccctta caccattgaa
     4021 gttggtcaga aactaaaact gggtggggcg aaaagtagca gtagtacacg taaatcaacc
     4081 gccaaatcaa cgactaaaac cgcatcggtt acaccgtcat cagcggtacc gaaatcttcc
     4141 tggccgccag tagggcaacg ttgttggtta tggccaacga cagggaaagt tatcatgccg
     4201 tattcgacag cagatggcgg caataaaggg attgatatct cagctccacg gggtacacct
     4261 atttacgccg cgggtgcagg aaaggtggtg tatgtgggca accagctgcg tggctacggt
     4321 aatctcatca tgattaaaca cagtgaagat tacattacgg cttacgccca taatgacacg
     4381 atgctggtaa ataatgggca aagcgtgaag gctgggcaaa aaatcgccac tatggggagc
     4441 acggatgcgg catctgttcg cctgcatttc cagattcgtt accgtgcaac ggcaattgat
     4501 ccgctacgtt acttgccgcc gcagggcagc aagccaaaat gctgatggcg aattaatcag
     4561 cagtcagcag cgcggcactt gctaaggaga gcgtaaggtt tataatgcct tacgcatctc
     4621 gaagcgggcg tagttcaatg gtagaacgag agcttcccaa gctctatacg agggttcgat
     4681 tcccttcgcc cgctccatga cacttcttaa agtctactaa cttactgaat cctaagggat
     4741 acggcttaac gccttccgat agttttactc actgtaacca acactgtgac taactttgtt
     4801 gaagtagtga gcagacctaa ggtctcagta catgctcaag tcaagaacct acctttatca
     4861 gcgtaatggt gtcttttaca tccgtcttag gatgaagacc actagtcgcc tgaccgcttc
     4921 gcttccttct cacaaccgtt ataaactagc gtcagtatct ttacggacca aggatagacg
     4981 taccgctatg gctcactcac ggcacatcaa gtcagcactt aaagcaatcc atgcagacaa
     5041 ccctaacgcc tcttatgagg agctacggga gcacctgaag accattgtcg agtgggaact
     5101 tagtgtcagc cgtgatgacc tgaacgaccc ggagtcctac cagctttacg ttgaccagta
     5161 cgatgacatt aagtccaacc ttcgggaagc tgtagcgacc gagcgtctga ctgtagacca
     5221 gcatcgctat attaatgacg ttatcggtgt gcttaaggcc tgtcaggata gactcaatgg
     5281 cgatagctct ggcctattgt cttaccttga gcctgagact ggcagtctaa gaccgtccgt
     5341 ctccctatct gtattggcgg aacctgaagt ccctgaacct aaagcactga ccctcgcgtc
     5401 tctcattgag cagtacgagc aggagaacgc ccagaactgg aagccagcga ccctaagcga
     5461 aaaccgagcg tcacactcta cgctaatcga gatattcgac tatctggata ttcaggatgt
     5521 cggcaaggcg acccgtgcag atatgctcag agttcgtgaa gtcctccagc agctaccgaa
     5581 gaaccgcaag caacgcttta agtctatgcc gctgtctgac ttgctgaacc gggagtctaa
     5641 gactgactgt ctggacgtcg ttaccattaa caataaatat cttatcaaga tggctgctgt
     5701 gtttaagtgg gcggtacgca acgacttgat agctaagaac ctgaccgaag gtcttgagct
     5761 gaaagtccct cagcgtaaag cctccgacgc tcgtgatgcg ttcagtcctg agcaggtagg
     5821 gcaactactg gtcgcagcta aagcgtactc gcagaagacc tcaggtaagc cataccatta
     5881 ttacgtcacc gctctggctg cgattacagg tgcgagactt aacgaggtgg cccaacttca
     5941 ggttaaagat gtcaggacca ctgaggctgg gaccgttttt atccatatca acgaagacga
     6001 tagcagcctg ccgggtaaga gtatcaagaa cgcccacagt gaccgctgtg tcccgttggt
     6061 agatggtgct tatggttttg tattggctga ctttatgtca ctcgtggaag accgtagaaa
     6121 gactgagggt gataatgcta tggtgttcaa tggcctcaag ctaatgaaga acggctacgg
     6181 tgagcaggtt agcaagtggt ttaaccgaac gttgctgccc aaagtcctcg ctgaccgtag
     6241 cggcttagct tttcactcgt ttagacatac agttgcgacc cagttaaaac aacatggggt
     6301 cgagttagcg tatgctcagg cgattatggg tcatagctca gggtctataa cttacgaccg
     6361 ttacgcgaaa gaggtcgagg tggaaacgct gaaagaaaaa ttagcggagt cattgtcagt
     6421 aaaaaaaata gacggaaagt aatcgcatag ggatagcgga atgtgaggac gtaatggtca
     6481 aagaattttt agttagcaaa gaaaataagc cttcatatga atgtatgaat actgctgaaa
     6541 cttgtttggg gctggctgga atggcttcgg aagattggca acgtgagcat cattgccgca
     6601 caggtataat atttactgct tttgctattg aggcaatgtt catcttctat agaaaacagg
     6661 ttgaccccgg ctacgataag acgcagaaag agtgcagaaa gacaatgcac aagaatacgt
     6721 taaaattgtg cggtataaac aattatatgg ggacaaaacc ttaccaaatt atcaaagagt
     6781 gtcttgaggt aagagacgct attgctcacg gcgattcgta tacttcgagc tttaatttct
     6841 cggcggatca tttggataac caagacgata ttgcaaaagt gtcctggcgg taaactctga
     6901 acaatttcgg ggattaactg ttgagcgcct tgcaaaatac attgaaatgg caaagcatat
     6961 tgaccatgag atttctgata atggttacag gccttctgaa ggttaccttc cagcagccga
     7021 gcgcagaagt ctgatgttag cttttggcgt cagtggtgtg tcaatgtggc ccagtgcttg
     7081 agcgataata cacctcacta tagaggaatg gacccttaac tatcactata ggggagagac
     7141 cctaagtccc caccttaata gcagtcacga cccatacaca cattcattaa gtctcaggct
     7201 tccggtagcg gaccgattgg aaaccatccg cagacgccta gtgttaaatc accttggcct
     7261 gagaccattc cttttaacgt aagctatctt cccttttgac aggcggggag cagtagacca
     7321 catccgactt taagttggct ctgtgataat cgctgcgcgg ataactgaag aaccaagaca
     7381 ccattactga ggtctgccat ccatttgtgg acaggcggaa actcaaagag gttagatcag
     7441 tggtgtctga ggttggagct ttgagtcttg accagcaggc tggaggagac catgagttta
     7501 tcttgttatg gattgtgtta tcacaattcc aataagaaga actttaagtc cagcctccac
     7561 tcccgccaac ccgaagtaaa attatggtcg ctatgatgtg gtctccatgc ttcgggtctt
     7621 actggaagta ctgaacactt tcgcgtcaag ttctccgaag ttcattcctg tttgactaaa
     7681 ctatcactat agggaccata tctcgcctct gacctcctga gagacggcaa gcgagaaacc
     7741 gtggtccacg ctagcaagct agccaccagc ctaaggcaag acagcctctg gtcagcctgt
     7801 gtgttctttg gtctcgtgac ttaagtctat caccacacac gctcggacat gctgtcgaac
     7861 cttcatctct atggtcatcg cagaccacta accccaatta cctcaagtca ttctgtttgc
     7921 gtcctgttgc ctgctggtct ccctttacgt gggggcttga gtaggtcagt ggggtgcgac
     7981 ttcagggtgc aaacaaaagg agtacacgta aatgtcccaa caagaaatca gcatcattaa
     8041 cctcgaccag cttgtgtcca tgacctcagt tgaaattgct gagctgacag gaaaagaaca
     8101 taaacacgtt ttacgagata ttcgtaatat ggtcgaagag cttaacgggg ccaaaactga
     8161 gcactgctca actttgagca gcgagcttaa cgggtccaaa tttgggctcg ttggcgagga
     8221 agtatacaag gacgcgaaag gtgagagccg aacaatgtat cgtctcgacc gtaagcatac
     8281 tttcattctg gtcgctggct attccgttca cctccgcgcc aagtgttacg accatattca
     8341 gacgttagag cgtcgagtcc ttcaactgga agaccagaag aagcgagcag ccattcagtc
     8401 cgctaaccgc cgaggcgtga cttggggcga ctactgcaaa acctatggtc tgcccgctca
     8461 gaagctaatg accgcacttc tccagcatcg tggcctcttt cgtaagaacc caatcagtaa
     8521 cgagtggtct gtaaatccga agtattccga ctgctttcgt atcattaagc cgtcagacca
     8581 gaagttctca gcaggtgggt ataacttccg gttcaacgct aaggggctgg aggtcttcgg
     8641 taagcctgag atggttgaca agatgcgagg catccttatt gcattcactg gcaccgacca
     8701 gcagaagcaa gagcacctcc tgaagctggc acaatccggt aaggtggagg gaatctaaga
     8761 tgatgattga cccgcgcaca cctgaggggc gcatgactct ccgttaccgt ggctaccgga
     8821 ctgaagtcct cctcagagag cttggtctag acccggaaga tgagacccgc cagcaccaat
     8881 cccgagacga gctaatagcc caactggtcg ctatgaaact gccgcttaac cgttagtagc
     8941 tcacctactc aatacctaat ttaaacacct tttgagatga ccgatgcggg gagacccgta
     9001 gg
//
LOCUS       DAJTQQ010000138         8918 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000138 DAJTQQ010000000
VERSION     DAJTQQ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8918
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.138"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO309_003659"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO309_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR5848656.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO309_003660"
     CDS             1828..3147
                     /locus_tag="OO309_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR5848657.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3445..3486
                     /locus_tag="OO309_003661"
     CDS             3445..3486
                     /locus_tag="OO309_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848658.1"
                     /translation="MVQLVDLARCVSF"
     gene            3524..4669
                     /gene="dosC"
                     /locus_tag="OO309_003662"
     CDS             3524..4669
                     /gene="dosC"
                     /locus_tag="OO309_003662"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR5848659.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="OO309_003663"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="OO309_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5801
                     /gene="ddpX"
                     /locus_tag="OO309_003664"
                     /pseudo
     CDS             5469..5801
                     /gene="ddpX"
                     /locus_tag="OO309_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5815..6711
                     /locus_tag="OO309_003665"
     CDS             5815..6711
                     /locus_tag="OO309_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR5848660.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6708..7682
                     /locus_tag="OO309_003666"
     CDS             6708..7682
                     /locus_tag="OO309_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5848661.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="OO309_003667"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="OO309_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8657..>8918)
                     /gene="osmC"
                     /locus_tag="OO309_003668"
     CDS             complement(8657..>8918)
                     /gene="osmC"
                     /locus_tag="OO309_003668"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR5848662.1"
                     /translation="ACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALK
                     SEVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2175 a         2181 c         2249 g         2313 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 gaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctggcgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     5701 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     5761 acagccgtgg cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg
     5821 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5881 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5941 atgcgactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     6001 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     6061 cgcgatctgt ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg
     6121 gtggtcgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6181 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6241 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6301 attgctattg tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta
     6361 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
     6421 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
     6481 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctgggtgc tcaacaaccg
     6541 agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg
     6601 tattgcgcat ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt
     6661 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6721 ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc
     6781 tcaacaatgt gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg
     6841 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6901 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6961 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     7021 ttaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     7081 taagtcgtcg ggaagccaga gctaaagcga ttgcactgct ggaagagatg caaatcccgg
     7141 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7201 taatgattgc gccggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga
     7261 cggcgctgga cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg
     7321 ccagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
     7381 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta
     7441 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7501 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7561 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcacctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggcaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttgctgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gatctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgacctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
     8701 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
     8761 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
     8821 ccactttatc cagcgacaca tcggcggtgg tatcaattga tgttggcgtg aatcccgctt
     8881 cccccagcat taatgaaagc gccattgaga aacatgcg
//
LOCUS       DAJTQQ010000139         8834 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000139 DAJTQQ010000000
VERSION     DAJTQQ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.139"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            47..742
                     /gene="minC"
                     /locus_tag="OO309_003669"
     CDS             47..742
                     /gene="minC"
                     /locus_tag="OO309_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR5848663.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            766..1578
                     /gene="minD"
                     /locus_tag="OO309_003670"
     CDS             766..1578
                     /gene="minD"
                     /locus_tag="OO309_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR5848664.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            1582..1848
                     /gene="minE"
                     /locus_tag="OO309_003671"
     CDS             1582..1848
                     /gene="minE"
                     /locus_tag="OO309_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR5848665.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2220..2564)
                     /locus_tag="OO309_003672"
                     /pseudo
     CDS             complement(2220..2564)
                     /locus_tag="OO309_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(2599..2718)
                     /locus_tag="OO309_003673"
     CDS             complement(2599..2718)
                     /locus_tag="OO309_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR5848666.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(2679..2864)
                     /locus_tag="OO309_003674"
     CDS             complement(2679..2864)
                     /locus_tag="OO309_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848667.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            2965..3138
                     /locus_tag="OO309_003675"
     CDS             2965..3138
                     /locus_tag="OO309_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848668.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3140..3484
                     /locus_tag="OO309_003676"
     CDS             3140..3484
                     /locus_tag="OO309_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR5848669.1"
                     /translation="MKKKILAFGLISALFCSTPAIADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            3488..3823
                     /locus_tag="OO309_003677"
     CDS             3488..3823
                     /locus_tag="OO309_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR5848670.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(3880..6201)
                     /locus_tag="OO309_003678"
     CDS             complement(3880..6201)
                     /locus_tag="OO309_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5848671.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6318..6527)
                     /locus_tag="OO309_003679"
     CDS             complement(6318..6527)
                     /locus_tag="OO309_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848672.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7278..8801)
                     /locus_tag="OO309_003680"
     CDS             complement(7278..8801)
                     /locus_tag="OO309_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5848673.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         2450 a         2067 c         1939 g         2378 t
ORIGIN      
        1 tcatcgcgcg ctggcgatga ttaatagcta attgagtaag gccaggatgt caaacactcc
       61 aatcgagctt aaaggcagta gcttcacttt atctgtggtt catctgcatg aggcagaacc
      121 taaggttatc catcaggcgc tgaaagacaa aatcgctcag gcccccacat ttttaaaaca
      181 tgcccccgtt gtactcaacg tcagtgcact ggaagacccg gtaaactggt cagcgatgca
      241 taaggcggtt tcggcaaccg gtttgcgggt tattggcgta agtggctgca aagatgcgca
      301 acttaaagcc gaaattgaaa aaatggggct gcctatcctg acggaaggaa aagaaaaagc
      361 gccacgtcca gctcccgcac cgcaggctcc agcgcaaaat acaatgccgg tcacaaaaac
      421 gcgtttaata gataccccgg tgcgttccgg tcagcgtatt tatgctccac aatgtgatct
      481 gattgttaca agccacgtta gcgctggggc cgaattgatt gccgatggga acattcatgt
      541 ctatggcatg atgcgcggtc gtgcgctggc aggggcaagt ggtgaccggg aaacgcaaat
      601 attttgtacg aacctgatgg cggaattggt gtccatcgca ggtgaatact ggctgagtga
      661 tcaaatccca gcagaatttt atggcaaagc ggcgcgactg cagttagtcg aaaacgcttt
      721 gaccgttcaa ccgttaaatt gatccctttt taacaaggaa tttctatggc acgcattatt
      781 gttgttactt cgggtaaagg gggtgttggt aagacaacct ccagcgcggc catcgccact
      841 ggtttggccc agaagggaaa gaaaactgtc gtgatagatt ttgatatcgg cctgcgtaat
      901 ctcgacctga ttatgggttg cgaacgccgg gtcgtttacg atttcgtcaa cgtcattcag
      961 ggcgatgcaa cgctaaatca ggcgttaatt aaagataagc gtactgaaaa tctctatatt
     1021 cttccggcat cgcaaacacg cgataaagat gccctcaccc gtgaaggggt cgccaaagtt
     1081 cttgatgatc tgaaagcgat ggattttgaa tttatcgttt gtgactcccc ggcagggatt
     1141 gaaaccggtg cgttaatggc actctatttt gcagacgaag ccattattac caccaacccg
     1201 gaagtctcct cagtacgcga ctctgaccgt attttaggca ttctggcgtc aaaatcacgc
     1261 cgcgcagaaa atggcgaaga gcctattaaa gagcacctgc tgttaacgcg ctataaccca
     1321 ggccgcgtaa gcagaggtga catgctgagc atggaagatg tgctggagat cctgcgcatc
     1381 aaactcgtcg gcgtgatccc agaggatcaa tcagtattgc gcgcctctaa ccagggtgaa
     1441 ccggtcattc tcgacattaa cgccgatgcg ggtaaagcct acgcagatac cgtagaacgt
     1501 ctgttgggag aagaacgtcc tttccgcttc attgaagaag agaagaaagg cttcctcaaa
     1561 cgcttgttcg gaggataagt tatggcatta ctcgatttct ttctctcgcg gaagaagaac
     1621 acagccaaca ttgcaaaaga acggctgcag attattgttg ctgaacgccg tcgcagcgat
     1681 gcagaaccgc attatctgcc gcagttgcgt aaagatattc ttgaggtcat ttgtaaatac
     1741 gtacaaattg atcctgagat ggtaaccgta cagcttgagc aaaaagatgg cgatatttct
     1801 attcttgagc tgaacgtgac cttaccggaa gcagaagagc tgaaataagc ccgctgtaaa
     1861 aacgcattta tcttcaaggc agagtcattt ctgccttgag tttttcatcc ctctcatcca
     1921 cgttgtggta aagcggcgag tattcttgct gatactcctc attgctattt ccacctcccc
     1981 ctttacacct taaggctgtt cccacgcaca gaacaaaatg tttatgcctg gttgagtaaa
     2041 taaccatgtt attagtgtgt atatactggg agatgataac cccgcaattt cattattcat
     2101 tgttctccca taggatgagg tttcccgccg taactggcgg gctttttttg ccccagaatt
     2161 ttctctttca acatcctgta aacgaaaact gcgccgaagc gcagtttaat cgttgatggt
     2221 cagaacgacc agttcacacc agccaccgcg ttccacgggg actccacacc ggcaccatgg
     2281 ctatacccca ccccgaaatg cccgcttaac gtactgctga atgaggcttt aatacctgcc
     2341 tggtatattc cacgtctgcc cgacaaatca ttgacaaaat taccgtcact attcactttc
     2401 acccgattat catcgacaaa ttctttgcgc acagccgcct tcaggcaggg ttcaatttcc
     2461 ataccgttcc ccagacgcat gttgtaactc agcgttgcac ccagttcacg atatatactg
     2521 cgggtatcga ctgatttcga ttccatgcca ttggataaat gatagtagcg tctaagtgac
     2581 ctaagctctg ctgcaacatc agcccgcagg caccagacca ggggattcat cctgaagaga
     2641 cagcgcaggt gtattgtgtt caccgctcac caaagacatc atgatgaaat gatgatatcc
     2701 cgcataagaa agaggcattt tttacacgca gtgcgctgaa gtgtagttgg ataaaaaatc
     2761 actcttttca gataatactg tcgtacgctt atctttagac aatgaggccg tcggaaaaat
     2821 aaaatgacta atgtaatcgt tgctgatgaa tatcttctgc tcacataaaa atcacacaat
     2881 aatattgata aatcagatta tcttaccttt acaacattgc gttaacagtc aatgacactt
     2941 cttgaataaa aatattgtca atcaatgagt tattcctcat ttaagattat tctcattcac
     3001 gtaaaaaaca taattcctat catcacagcc acgctcattt tgaattatct gaataatagc
     3061 gagcgttctt tagttaagca gatcttaatt gaagacgaaa ttattgtgtg tgcaacttac
     3121 ttaataccgg acatctaagt tgaagaaaaa aatacttgcc ttcgggctta tctcagcatt
     3181 gttctgctcc acgccagcga tagcggatat gaaccgcacc acgaaaggtg cattgctagg
     3241 cgcaggcgtt ggtttactta ctggcaatgg tgttaatggc gtactgaaag gtgcagctgt
     3301 tggcgctggt gttagtgcag taacagaaaa aggccgcgac ggtaaaaatg cgcgtaaagg
     3361 tgcaaaagtg ggtgccgccg ttggtgctgt cactggcgtt ctgaccggca atggcctcga
     3421 aggggcgatt aaaggtgccg tgattggcgg tacgggtggt gcaattctgg gtaaaatgaa
     3481 ataaggaatg atgatgaaaa aatttgcttt actggcgggc ctgtttgttt tcgcaccaat
     3541 gacctgggcg caggactaca atattaaaaa cggtttgccg tcagaaacct atattacttg
     3601 cgcagaagcc aacgaaatgg caaaaactga cagcgcacag gttgcagaaa ttgttgcggt
     3661 aatgggtaat gccagcgttg ccagccgtga tttaaaaatt gagcaatctc cggaactgag
     3721 cgccaaagtg gtagaaaaac tgaatcaggt ttgcgctaaa gacccgcaaa tgctgttgat
     3781 cacggcaatt gatgacacca tgcgtgctat cggcaaaaaa taatatttca tatgtaccgc
     3841 gtcgatgaat acgacgcggt tttttagcta acggcgggat cagaacttat atttcaggcc
     3901 caccatcact gcggtgtcat tgtagccatt atcacccagc tgcacgccca cattccccca
     3961 gacgctggcc gctggattca ggttgccatt tacgccaagt ttaatttccc ccaaattact
     4021 caccccatct tgttcgactt tcacaccgtt cattgagaca gcaaaatctt tactgttatg
     4081 gatccagttc atttcaacaa aaggttcaaa gtcgtcaccg tgcggaccgc tgtttttctc
     4141 ctgagtacga ataaaggtgc ggaagccgag tcgggtctgg atgttgttat ttgcgtcatt
     4201 ctcaatacgt gagccattgg cttccgtgtg cgcattatga tcgacgccca tccagatagc
     4261 ctgtgcctgt ggctggaaga tccacgtata tttcgccgct tccagaccaa aggatttatt
     4321 cagggcaaaa ccgtaacccg cttccagcga gacggttgca cctttcgcgc tataggattc
     4381 ataggctaat tcatcccctt tcacggagtt tttaaaccag ctatattgcg cccaggagtc
     4441 aatgtatgcg ccttttttac tgatgtcatc ggcaaaccaa gtggcataca gccctgcgct
     4501 atagcctctg acactccctt tcgaacgata atccgacacg ctggaatgag ttaagttgta
     4561 gtcgcgggca taaccagcca tcactcccaa acgccaactg tcactatcgg taaaaccacc
     4621 ggttaacaga tcgcccccta actgcgagac gtagcgatgc gaggtcgttc tcaactgtcc
     4681 gttgctgtca cgccaggcat tatgcccgcc aatttgacgt agccaaagtc ggcttgaacg
     4741 ctcctgttca gttacaggat cgatgtagcg cgtttcaccc gcacggtcgt tcagacgcat
     4801 aacaaaaaga gtgttagcgg ctgccaggtt ggcgacgtaa cttccagctt cggggcgcag
     4861 gacgggggta ggcgcaacag taggcgctga acttcctccg gagtttgttt caccgccgct
     4921 cgttgcctgg aagttagtca gataccagtt tttattggtc gcttcggtat tacctcgggt
     4981 taatgaatat tcgtaggcac cggcgaaaat cctcgaatcc ttaataaatt ccccatttga
     5041 ttccccctca acgctgataa tctctatccc atttaacgtg taagctccgc tgcctcctgc
     5101 attggtaact ttaactcggg ttgttccaga ggtatttcca ttaataacga ctttatccgt
     5161 agctgaatta tcatcatcca attcagttct gagatgcaat acgccattat taccaacgta
     5221 attttcagtt attgttaatc ttgttggttc gacatcccgt ccatctgccc gggaaatata
     5281 gaccgtacta ttatcaaccg tgaggttgct caccgttgag tcagctttaa tatcccaggt
     5341 actattatct gaaagcgaca gataggtatg agtgttatca tctgttgaaa tattagctga
     5401 acccgttatt tttgaattag ttgcatcgac ggtcaaatga cctttaatat tattgataaa
     5461 aacatctgca gccgatgata cctcactatc cgtaatggtc acgttactcg tctcatcttt
     5521 atggtagagg atttcatttg ctgaccgtag tgagctacca gataaaatat ctaccgttgc
     5581 ttcctgacca tttatggaag actccagcac aattccagac ccctgatgcg cgactgcaga
     5641 tgtatttttc agcgtcaccg tgccgccatt ataaatttta gcaatcgcgg cgtcaggacc
     5701 cgttgtataa atttctccct ggttaaatga aactgtcccc tggcgggcag cgatggcatg
     5761 agcattgtcc ccatgcgtgg tgatatcaac atcggttaat gtaacgctgg cattggtata
     5821 atcaagcgtg tcaccagcct tgacgacagt gccattagcg tacgcggcat aactttctgt
     5881 tccgtgggtt gtgatctccc cttttgccat atccagtttt gctatggcat tattttcatt
     5941 acttcctgta tcggcagtac tttcttcatc aagagaagat acacacgcac acgcgcttcc
     6001 gggagatgca cacgcaagaa cgccgactgc gtgattaccc gcagtaacaa taaatgtatc
     6061 atctccgtca gaaaaagagg gggtaaccga accgtcagag ttgactctta ttatggtgtc
     6121 agtattcagg tttccactcg tttgacttag caaaccaatt gagtaatccc ccgttgtgac
     6181 aatcgtgttt ttaccctgca aaataacagc accgccaaat tcacttacga ttgcgctagc
     6241 tgttaatgct gggcttagag gactattgtc agaaggatat aaaccgcccg ttgttacaat
     6301 gacgccattg ttaatggtta acttactacc ctctcctgca acggataccc gtaacttcca
     6361 ttacccgttg ttataactag cgcattatca atatctttta cttttccatc ttttattgcc
     6421 acagcatgtt tatattcatc gtgctgaata aactgttcag catatgcatt acccaccgcc
     6481 agagcaataa gtcccaaact aaacccgggg agttttttca gtttcataga ttaactatcc
     6541 attcattgga ttaacaaaca ttcctggtca aataacctat gagctcagat taccaaaacg
     6601 gagggtaaag acaaaaacaa aagcacaaaa aatttaaata gataaacagc gacaatatat
     6661 aaatgataaa taagtcggca ttatcatttt tcaccttatt ttcatgacat tgatcacttt
     6721 ggggtgacta ttttgtttaa caaataactc ttcgacatca aaattattaa tagttgccat
     6781 ggtaattatt tcggtgatat ataaaaacaa ctgtgataac accaaggttg acaactattt
     6841 gcgcaataat tcctttcctt caatgatcca ggcgttcact gccagcaacc cattcagccg
     6901 catactgaca gtgagaaact gaaagattat tgctgatccc tgtggctaac gagtctttct
     6961 cttaaatcga ttaaccagat tgatcgccag ccaaacaagg gcattaagca aatttatcgt
     7021 cacaagcact tctcccagcc cggtactggg ttttgcaata aggatgacca ggagggtgat
     7081 cagcataaat gcaatggaaa agatgatata gagagtgaaa taatccagat tattactatt
     7141 attcatgcta ctaatggcaa tcctgtcaaa actttcaaac aattatagca aacaaaataa
     7201 tgaacaatag taacaagagg caacgagcta attagctgtc tataattaca ccgcactatg
     7261 gccttactga tcaattttca ctcaaccaca accttcacct tcggtttaga aaggataatt
     7321 ttgaccaggt cgatgtatgt aacaggttta tagaaataat aaccctgctg aaatgtgata
     7381 ttattttggt tcagatagcc aagttgttct ttcgtttcga caccttcagc gacgatactg
     7441 atcgaaagtt tacgcgccag atccaataca caatccagaa ttcgggttga ttctgggtct
     7501 gcgtttacgc ggccaacgaa actgtgatca attttgatat aatcaatatg caggtcatga
     7561 agataagaaa gccctgagta gccagtaccg aaatcatcca gcgcgatgac aaaaccattt
     7621 tcatgcagaa tgttcaaccg ctgaaccaga ctttcatcaa cattcaatgg ctcacgctcg
     7681 gtgacttcca gaacaaggtt taaatcgcgg cgggtaaaac tgtcacggaa atttaaacac
     7741 tcgtcgacaa acgtcggcga aataatatgc gaagcgctaa aattaattcc aatatgaaaa
     7801 ccttccggca gtttactcgc gatagcgttc atctgtctgg caacctgatt catcaggctt
     7861 tgtgtaagcg gaacgattaa tccggatttt tcagcaagtg gaataaatgc cgcgggtgaa
     7921 atatatccac cgtgaggttg tttccagcgg gctaacacct caactccccg caatgtccct
     7981 tcccgaccat ttaccacagg ttggtaaaaa gggacgatct ccccattatt tattgcccgt
     8041 cgcaggatct cttcaggcgt tgcgctttta ttgaaatatc tatcaagcag gaaggcagcg
     8101 gcgctggcga ttaacagaat aaatattaaa atcccaaagc ctttatcgat aagtctgtag
     8161 aagctaaaga gtggcggcgg gttaaagata atagtgaaat gatattttgt ggagtgaacc
     8221 ttctcggtat agggataatg tccattaagc gtcatcacat cagccgttgg tccaataatg
     8281 tcatccgcca cgcgtaatac ataccttacc cctttcaagg gtacattaag cgccccgcga
     8341 atatgttgat cacttatcgt aaccaaaatg cgcgtatctg caaattggct ctgatagagc
     8401 aggataggta atctattaac ggtgtcgatt gccggagcca acagtaaatt actgtccggg
     8461 acaacaggaa tacgagacag caggacccga ttcccaggca gggatgtaca ccagactatt
     8521 ccctgtttga gaatgatgat tgtgcgcagg tgaggtttca gagctgcttc agtgccaagc
     8581 tgatattgct cctcgaggtc gcattccttc ccggcaatgt taaccgctgt ttgagtcgcc
     8641 tgtgacgctt cttcaagtat atgattgata ttgttggcgg catatcttgc tcccgccagg
     8701 tactctgctt ttgcggaata ccagagctgg atgttcaaaa tagcgacacc ggtaataaac
     8761 aagcatatcg caacgaggat gggtatgagt gtattgcgca tttgcttgtc ccggccaaag
     8821 ggtttaatac atga
//
LOCUS       DAJTQQ010000140         8782 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000140 DAJTQQ010000000
VERSION     DAJTQQ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.140"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(1..751)
                     /locus_tag="OO309_003681"
                     /pseudo
     CDS             complement(1..751)
                     /locus_tag="OO309_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(802..1377)
                     /locus_tag="OO309_003682"
                     /pseudo
     CDS             complement(802..1377)
                     /locus_tag="OO309_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1381..1781
                     /locus_tag="OO309_003683"
                     /pseudo
     CDS             1381..1781
                     /locus_tag="OO309_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2184..2765)
                     /locus_tag="OO309_003684"
     CDS             complement(2184..2765)
                     /locus_tag="OO309_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848674.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(2812..6669)
                     /gene="sigA"
                     /locus_tag="OO309_003685"
     CDS             complement(2812..6669)
                     /gene="sigA"
                     /locus_tag="OO309_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR5848675.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(7030..8034)
                     /locus_tag="OO309_003686"
                     /pseudo
     CDS             complement(7030..8034)
                     /locus_tag="OO309_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            8019..8396
                     /locus_tag="OO309_003687"
                     /pseudo
     CDS             8019..8396
                     /locus_tag="OO309_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8435..>8782)
                     /locus_tag="OO309_003688"
     CDS             complement(8435..>8782)
                     /locus_tag="OO309_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5848676.1"
                     /translation="LVPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGA
                     WLIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIA
                     LAAQSSFSQQKQN"
BASE COUNT         2120 a         2092 c         2033 g         2537 t
ORIGIN      
        1 ccagactgac cgcccgcagg gcattttcag cgatgttgtt gtcgatttcc acccagccat
       61 tactgcagta cacgttcagg gccttccact gtttcagtag gtatgcgaac gcttttgccg
      121 tatccgagtg acgcgacagt gttttcatct gttgctgtat ccggtcatac agtgactgca
      181 tcagtggcgc ggctcttgct tttcttgccg ccagacgctg ttctgctgaa catccccgga
      241 cttctgcctc gatggtatac agttcaccga tacgctgcag ggcttccgtg gtgatgtcgg
      301 ttggcactct tgcatgcaca tcgtggattt ttctccgggc atgggccata cacgcagctt
      361 ccgttattct gccggattcg tataacgccc ggtaaccacc gtaagcatct gcctgaagca
      421 caccgctgta accggctagg tgattctgtg gatggatacc tttccggtcc gggctgtacg
      481 cgaaccagac cgccgggggc atctgtgaac cggcgttacg gtcatcacgg acatagaccc
      541 acagccgggc agttcttgtt ttaccactgc ccggctcctg gaccggaacg gggatatcat
      601 ccgcatgaac tttaccgggc atcagtacat actggcgcag gacgtcatac agcggctcca
      661 gcagttcagc aacggcaccg gtccagcgac ccagtgtggc acggctcagc tccacgccct
      721 gacgacggta tatttctgac tggcggtata actgtcaacg acggatgaaa agtggctctt
      781 ccgcaacctg ggtggagatt ttcatgccag tggactcatt accctctgcc tgagtagttc
      841 aaatcctgct cgaccataca tctggcgttt cagcatcttc ttcaggcgat ttacatgacc
      901 ttcgacaaca ccattactcc agcgactact gattgcctca catatagctg cagcatcagc
      961 ttccatcccc gccgccacgc gccgaaattc tgctgaaccg ctttcgtgga ctcgagtgaa
     1021 ccaactgctt agctgtgatt tattttgggt tttcagaata cggtagaact cgagtgccag
     1081 ttgctgcgct attttcagct caggttcttt ttcacacatc agactaataa atcgggaagc
     1141 ataattttct tcatccctgg ttattctcca gggcatcaac cagcggctta cccgggagac
     1201 tgaaggaagt ctgacggcag tagtaaccgg tgggttccag cctttacgcc atttggcaac
     1261 cgcatccctg acgatggttt cactgcctgt aaatccccgg gccaccattt cccgccatat
     1321 ccggctggcg ttataattac cactttctcg ttgctctttt aaccactccc tccatggtgt
     1381 cagcgaaaaa cgaaaccgct gttgaaatcc ctggaaagct ggttgcggaa aagatgaaga
     1441 cccagtcgcg acactcagag ttggcgaagg cgttcgcgta cgcacttaac cagtggccgg
     1501 cactgacgta ctatgcgaac gatggctggg tggaaatcga caacaacatc gctgaaaatg
     1561 ccttgcgggc ggtcagtctg ggtcgtaaaa acttcctgtt cttcggctct gaccatggtg
     1621 gtgagcgggg agcgctactg tacagcctga tcgggacgtg caaactgaat gacgtggatc
     1681 cagaaagcta ccttcgccat gtgcttggcg tcatagcaga ctggccggtc aaccgggtca
     1741 gcgaactgct tccgtggcgc atagcactgc cagctgaata acacatcccc gtcaatacgg
     1801 ccctcgctgt acgcttactc atcacctgct attaatcccc gagtcagtac ttcgcggcct
     1861 gacgcgatca ccctactcag tacttcagag cccgcctccg gtacctgcct ggcgatcgcc
     1921 cctgtgcaac aaaataaatc aaccgttatc aaaccacgat cgttcgtatg cgccgccgat
     1981 gacgcgcgcc tgaaaaacgc gtcaatatga gttccccgga agcttacgat aatacgctta
     2041 tgaacattac aacacgctca tgcggaacct ggatggggga aggaacggtg aagctgttag
     2101 cgaatgaccg gcgtttatac aaacgccggt catgatgaat gatatttcag aatatacctc
     2161 gggctaaaat accgggacag agatcaggcg tgattaatct gcagcatttt taataacaga
     2221 agtactgagc ttactttacc tttgatgatg taatagttat ttccgtcggt actcattttt
     2281 ccctgaaata atgtcacgga tgtttcttcc tgctgatcct gcaggcgcag tcgtaaggtc
     2341 atatttttaa tgagctgaga tccgggtggc aaggggtatt tgctgtcaga tactgggcga
     2401 ctgagtctga tgtggcgcgt atacagcccg ttttcgtaca gggtggcctc tgttatggtg
     2461 tcagtgacca gcatgtggga gacaatgctg tattcttttt taaagcaccc accagaaacg
     2521 gtgattgtaa tgataatggt cttctcgatc gagatgtaaa tacccgtttc gttatactgt
     2581 ccgtatcgga accatgagaa ctcagttgca ttccagccat gctccagaat atgtgcattt
     2641 ttttgcctga tagtttcttt ttttattcgc tcatgtttaa ggcataacag gcagataaat
     2701 aatgtcagaa aaagaaagct aataatgaga gcaagagcga tatagtctgt cacagggtaa
     2761 tacatattct ctccggtaga agaagggccg caaacgcggc ccgggctgtt atcagaaaga
     2821 gtaacggaag ttggcgttga tcgcgttatc cacgttgtat ttaccaaatg ccgatttctc
     2881 aaactcaaga ccgaagcgaa gattatcgcg aatttcggcg ttgagaccaa cattcatgag
     2941 aatacgaccg tctttttcac ctttgatacg tttctcaccg gacgcatcac gcagtacggt
     3001 ttcaccgttg gcaaacaggt caaactggta gccaaggccg gcgcgggctg tgactttcca
     3061 gtccttaccg gagaaggatt tacccacatc aacaccggta cgcccaatca gcggattaaa
     3121 gtccttatcc ttcatggtca cgctcattcc gcgatcctgc cagtcgaacc gtttacctga
     3181 tacggcccca taaaccagtt ctgcctgcgg ctcaatccag gagtcttctg ttacatgata
     3241 gcggtagcct gcttcagcac ccacatacaa ggaatgagaa ctgtagtctt ttgttccgag
     3301 tccagcaaag gtcgccgagt actcattatc atggtgaaca tacttactaa tcaggtcgat
     3361 ataggcacca gagtcaaata tagcggaagc atacagacct gccccgacgg acttcgtctt
     3421 gccactgaat gcattactgc tggcatgact gtcggtatac gtcatagtca gaccagtgaa
     3481 aaggtcaagt ccatccagct catgtttttt atccacacca atctgcacat gggtgtagtt
     3541 gtcactgtat ccaccagaag ctgaacctgc tccgctcatg atgcgggccc atgcaccggc
     3601 ctcgccattg gtgtcacgca gatcccccat tcgtttattc aggttgttga cttctgtcag
     3661 gaaggctttg taattaccgg acatatacga ttttgctgca ccggaagatg ctgtattttc
     3721 agccacctta tatccggtca gggtccacgt ggtatgttcc tgatcctctt tcgtgctgat
     3781 cacaggagat atgttgctga aacctatggc ctgagtactg gctttaaata tctctttatt
     3841 gcttcctttt ggggctgtta tcaggtcaac gtttaatcct gagttattat ttcccacttt
     3901 ctgtatatcg accgacagac tgttattgct tccggttaac tgatttttaa caatgagttt
     3961 atccgattga tttacagact ggctcagatc agcccgcata atcacagcag tattagtggc
     4021 atccagtgtg tttactgtca cagtatggaa acccgaattc tctatgttac taaacaacaa
     4081 aagactgtcg cgggttttta atgattgcag ggctgactgt ccgtttactc tccataaggt
     4141 gttatacatg ctggctgagg ctgcaggagc atcaatacct ccggtataag aagtatcaaa
     4201 gccacccagc aaacctgtag ccagcggtgt gtgtgtggtt ttgtccaggt tatcttcttt
     4261 atactggtca acaccaaaca gaatagatgc ttttttgtct gattttatat taccattgac
     4321 ccaggcataa ttttgagcct ttaacgtggc accatcttct gtcatattat atccatctga
     4381 catatagatt gccgggctgt atttattatt ttctgtaggg gtgcctgtta atgtcagggt
     4441 tccggccagt tcaatagctt tatctacata catttttccc tgggcggtaa cagtagctcc
     4501 tttgttgata atcaggtctg tattaattaa ggataatgca ccagtcatag taacagttga
     4561 tttatcacca acagttattt tgctgttttg agcactcaga ctgccgttga aactactttc
     4621 gcctgctgaa tccccggagc gaagctgttg tctgaatcca aaaccttcgc cagtaatgtt
     4681 tcttcctgag aacatgtcaa tgtatgcagt tttatttccc agaacaattt gagagttttt
     4741 agcctctatg tgtcctttta cattagcatc ccggcctata ctcagggttg cgttttctaa
     4801 atttaatttc cggaaattaa atttacgact ttcccagtcg ggctgggaaa aagaggctat
     4861 ctgattgttg gacttatatt ccgtattgta acgtttattt acagtgcttt cctggtcctt
     4921 tatggcagca gaatagtctt tttgacataa aaaaggaatt ggacagttgt tattgccttc
     4981 tttaaatatg gcatgttctg tcgcatgtcc ttgtaatgtt aactgtgcat tccggacaga
     5041 gatatcgttt ttgatatcga ctgagccatc aaagatcaga ttggcattat tgttatgttg
     5101 ctgggtagta ttgataatat gatttatgct tatattacct gatacattcc catggtacat
     5161 atatgcatca gtattagtaa gtgataatgt tgattgcttc acgtttgagt tcgttacggt
     5221 tgttcccgca tctgttgctg cgattttctg aaatgactgg tcatagccgt ttagatccag
     5281 tgttccgcca tttctggtaa aatagatttc gccattaccg ctttcgctta aagcgtcttt
     5341 ggcatttatt tttaccgtac ctttacctcc ggccatgtaa attttattga aggctttttc
     5401 agcactaagg atgacagtcc cattacctat tttaaggtta tttccctgtg ctatttttac
     5461 atccagagta ccactaccta ttttatgcag attatctccg gcaacgccct taacattcca
     5521 ttcaacggtg gtattattat caatatctat cccggcacct ttataagact tatcttttcc
     5581 gtacacttta tatgtctggt tcttatcgaa tataagcccg ccaatgccaa gatcggtatc
     5641 ctgcttgagc gttaataccc ctcctcctga gacagacagg tccttatctt ttataatcga
     5701 tgataactct tgttttttac catccagctg catagtgttt gtgaaattct tttttaaatt
     5761 gtttaccaga atattattaa ataacgagta gtttgtccag gctatattgg tactactgat
     5821 aaaaccaact gtactggtaa caccatggaa aacccatttt tgttgttctt tatcatatag
     5881 ataagaacca gaaccactat cgccaaaaat tggtgagttt ttaaaagggg ttctgttggt
     5941 ataaatgtaa ctcttgtttt tccagtccag attaaaaaga cttgcgctta acaagtcggg
     6001 attatagttt tgtccaaaca gatattgttt gccgtttaat atcgttgagg ttgttccggc
     6061 acctgctctg aagccaatta tttgttcctt accgttgtag tttacgccat atcttttcat
     6121 cgcatcttcg ggagaaagtg agaagtcggc actctccgta acccctgttg tttccacgac
     6181 gaatttattc agacgatgaa ccgaaaagtc gccagatgat actctgttac ttgctttata
     6241 tgctgtctga tcccatgact gtgttgttat tgcatgatgc tgtgtaccgt tatgagtcgc
     6301 agtaacactg tacgcacctc caattgatgt tgttgcacct ttgttggaaa cagcagaaaa
     6361 atcaggtaca ggtagttctg gaaaatgaaa tatctttcca tctttcatca ttaattgaac
     6421 atttgttgca cccggtctga actctcctct gttttgagca aggtcaagat aatcgcgggt
     6481 ccatatatta tttatatcca gcattgctgc aaatgctgtt tcgccataac ttccatatat
     6541 tgaggataaa ataataccga ggataacttt ctttcttgat gtaccgacac taactttccg
     6601 ggtcagttca gaaacagcaa ctaatccacc tgtaatatga ctatatttca gtgaataaat
     6661 tttattcata cgtgaactcc gatatttctg agttgtggtt atgggatagc atttaattac
     6721 aacggggaac tatcagtcat ggtacgtaac tgataagggt ataatgataa tcactgtcag
     6781 ttatatttgt atgacagatg taacaaaatg aaaaaaaaga taagcacaag tgattgattg
     6841 gttagatatt gatcacttgg tgtttgtatt ttgaattttg cgatcaaaat ttgatctttg
     6901 ttgtttccgg tcaatccttt acaggttttc tgtattatcc gggggagtta agtctgtcga
     6961 tgtccgacag acttttgatg atggaaacaa catgcagcga taatgtgcat gctgttttgc
     7021 aggtggaact cagaaggtga actggaactg tgcgaccgca ccgtaagtgt tgtccgtgcc
     7081 gatcaggcct gcaacatcaa ggtgaaccac atcaaacggt gagatcccca ccccggccgt
     7141 gaatgcactg ccgtcgttac cggccagatt ctgacggtaa ccggcgcgca gctgtgccca
     7201 tttccaggca ttaaattctg tgccaatggc ggcaaactga cggttgctgt cggaggtgaa
     7261 accgctggcc ggggtcagat ccacatcaaa tgcagtggtg aacatcgcgt tgtgccagga
     7321 gacaccggcc gtcacctgcg ggcggatcct gaaggtttcc gtgataccgt tcacttcttt
     7381 tgtctcaata ctacgggaga tcaggttctg tgcgaccagt cccagcgtcc agttgtcatc
     7441 caggtccgta ctggcaccga tgtcggcgtt gatgccattt ttcgtgttgt ggtagcggtc
     7501 gcctttgaag gcgctgctgt cataatttct gaccagcacg ttgtagttga acaggtcgac
     7561 gcgctggaat ttggggggga agcccagaga ccatttctgc ccggccgttt ccagttccgt
     7621 agccagagca atacccacat cagtgatcag tgccgtgcgg gcacaggcct gtgatgtcag
     7681 gcggcttttg tccacctcct gcccggtgct gtttgccacc tcttccaggt aagtgaggtc
     7741 agcatcagag actttaccgt cgacactgac ggtgccgtgt gatttaacca tcagtgcggc
     7801 agagaggcga tcaccgggaa gggccgccac agcagaaacg cccagctgtg cggcggcatg
     7861 tgtgttacgg aagtcccgga ggtgttcttt caggcgtgcg gcagcctcag gaacaccgtg
     7921 gttactttcg atggcgcggt cgaaggcctt ccactcatca ctgatccggt cggcgttgtc
     7981 ggtgatatta tccggattgg agagctgcgc gccgacggac ggaagcagga tgctgggctc
     8041 atgcgcgacg caaaatccac gatgtatata tcagtactaa aagcgcgacg gcggaagaag
     8101 ctctgaaact aatcggtgaa ctgtacgcca tagagcacga tatacgcggg ttaccggtgt
     8161 ctgaacgcct ggcggtcagg caaatgcaga gtagaccgct cctgacttcc ctgtataagc
     8221 tgatgcagga gaaagaacac acgttatcga agaaatgtcg tctgggagat gcgttccggt
     8281 atatcaggaa gcactgggct gcgttgtgca acttctgtga tgatggcctg gctgaggtag
     8341 ataataatgc agcagaaaga gcgcttcgtg cagtctgtct cgggaagaaa actttttcga
     8401 ggatcactca ttccgtgtct gactgcttca gggtttaatt ttgcttctgc tgactgaatg
     8461 atgattgggc agcaagtgca atatcccaca taaatccagt caactcacga gccaccgccg
     8521 taatggctac ttgtgatttt ttcccttttg ctgtcagagt tcggtaacga gaacacaatc
     8581 gttgttgagc tttccatgca atatctttgg aatattgtgt tacagagtcc ggtcggtaat
     8641 atataagcca tgctccaact tttgcaggtg tacgataaga ccaggcggct tcgtaaagca
     8701 gacgtctcag ttcgctgttt ccaacttttg ttattcctct gggacgaata ctgtttccac
     8761 tggaatattc acctggcacc ag
//
LOCUS       DAJTQQ010000141         8739 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000141 DAJTQQ010000000
VERSION     DAJTQQ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8739
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.141"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..428
                     /locus_tag="OO309_003689"
     CDS             <1..428
                     /locus_tag="OO309_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR5848677.1"
                     /translation="LNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFAVQ
                     SGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKL
                     NVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(575..2737)
                     /gene="uvrD"
                     /locus_tag="OO309_003690"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(575..2737)
                     /gene="uvrD"
                     /locus_tag="OO309_003690"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR5848678.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2821..3537)
                     /gene="yigB"
                     /locus_tag="OO309_003691"
     CDS             complement(2821..3537)
                     /gene="yigB"
                     /locus_tag="OO309_003691"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR5848679.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3537..4433)
                     /gene="xerC"
                     /locus_tag="OO309_003692"
     CDS             complement(3537..4433)
                     /gene="xerC"
                     /locus_tag="OO309_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR5848680.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4430..5137)
                     /locus_tag="OO309_003693"
     CDS             complement(4430..5137)
                     /locus_tag="OO309_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR5848681.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5134..5958)
                     /gene="dapF"
                     /locus_tag="OO309_003694"
     CDS             complement(5134..5958)
                     /gene="dapF"
                     /locus_tag="OO309_003694"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR5848682.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(5995..6198)
                     /locus_tag="OO309_003695"
     CDS             complement(5995..6198)
                     /locus_tag="OO309_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5848683.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6387..7865
                     /locus_tag="OO309_003696"
                     /pseudo
     CDS             6387..7865
                     /locus_tag="OO309_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7862..>8739
                     /locus_tag="OO309_003697"
     CDS             7862..>8739
                     /locus_tag="OO309_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5848684.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPG"
BASE COUNT         2026 a         2385 c         2244 g         2084 t
ORIGIN      
        1 cgttaaatct cgcttcaggt gaagggaatt tctttatccg tcctggcggc gtgttttatg
       61 tcgcgggaga taaagtcggc atcgttcgtc tggatgcttt taaagccagt aaagatattc
      121 agtttgcggt gcagtcagga ccaatgttga tggaaaacgg tgtaattaat ccgcgtattc
      181 atcccaacgt cgcctcacgt aaaattcgta acggtgttgg gattaataaa catgggaatg
      241 ccgtgttttt gttgagccag caggcgacaa atttttatga ttttgcctgt tatgccaaag
      301 cgaaactgaa cgttgagcag ctgctttatc ttggtggcac gatttcacat atgtatatga
      361 aaggcggggc aatcccgtgg caacgctatc cttttgtcac catgatttcc gtcgagcgga
      421 aaggttagaa tgatagcttt gtgtcagata ctaaatatgc tgctgatgcc ggatgcggcg
      481 taaacgcctt atccggccta catgacgttg caatttattg aatctgaatg attttttagg
      541 ccaaataagg tgcgcagcac cgcatccggc aacgttacac cgactccagc cgggcgtaag
      601 ctgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg ctatgttcac
      661 cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgt acacgctggc
      721 cgagcttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga tggctgaccg
      781 ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc aactcgccaa
      841 taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt tccgcgtagg
      901 tcagcgtcag tttctgcatc gcgcgggtta cgccaacgta ggccagacgg cgttcttctt
      961 ccagacgccc gccttcatcc agcgacatct ggcttgggaa catgccctct tccataccga
     1021 cgataaacac ctgcgggaac tccagacctt tcgccgagtg catggtcatc agttgtaccg
     1081 catcctgcca ggtatccgcc tgcccttcgc ctgcttccag cgccgcatgg gataggaacg
     1141 cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc gttgccgtca
     1201 ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc tgctcgtaca
     1261 tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc ggcatatcgg
     1321 cagtttcctg cgctaaggcg tcgatcagtt ccataaaccg ctgtaaggcg ctggcagcac
     1381 gtccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt gttaactggc
     1441 gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc cgcgttggcg
     1501 tattcaccac acgctcaaag gccgcgtcgt cgttacggtt ggcaatcagg cgcagatacg
     1561 agagcgcatc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa atacggtacg
     1621 gcatactcgc ctgcaacagt gcttcttcca gcacacgcga ctgggcgtta ctgcggtaga
     1681 gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca ggttttgatg cggttaacca
     1741 caaaacgcgc ttcatcgagt tcgttaaaag cgcaatagag ggaaataggc tcaccgtccg
     1801 cgccatcggt ccacagtttt ttacccagac gcccgttatt gttttcaatc agggcgttag
     1861 cggcgctcag aatattgctg gtagagcggt agttttgctc cagacgaata atttcggcac
     1921 cggggaaatc attaaggaaa cgctgaatat tctccacctg cgccccgcgc cagccgtaga
     1981 ttgactggtc gtcatcaccg acgatcatca ctttgccggt gtcgcccgcc agcaggcgga
     2041 tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggata ttggtaaaac
     2101 gttcgcggta gtgttgtagg atatgcggct tgttaagcca cagttcgtga gcgcgcagca
     2161 gcagctcggc gaagtccacc aggcccgcgc gatcgcacgc ttcctgatac gcctgataca
     2221 ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc ggacgcaggc
     2281 cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac tgcttctcgt
     2341 cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttcg ctatcgagga
     2401 tctggaaatc ctgcggcaga ttggcgtcca tatggtgcgc acgcagcagg cggtgcgcca
     2461 gcccgtggaa ggtgcctacc cacatgccgc cctggctggt gcccatcagt tgtccgatac
     2521 gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc gagtatggcg
     2581 agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc gtcttaccac
     2641 tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct tcgcgttgtt
     2701 tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg taaaaatagg
     2761 ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca gatttgctga
     2821 ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgtggca gtaaacggct
     2881 gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct gcattccgct
     2941 gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga tctcgccgat
     3001 cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt ttgagcgccc
     3061 gtgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca gttccggctg
     3121 ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt ttaaggtgtc
     3181 gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa tcattgctgc
     3241 gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg cttgttcaat
     3301 cgaacgaaaa cgccaacgtg tcacgtcgtg ataaatctcg ggttccgctt cccgtaccgc
     3361 ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat gataattttg
     3421 cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat cgtaaagggt
     3481 atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa aacgcattat
     3541 ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg ttgaaaatca
     3601 agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagtagctc ctgcacacca
     3661 cgaagatcgc cgctcgactc cagcatatgc gtagcgaacg agtgacgtaa tttatgtggg
     3721 tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt ctgcacatta
     3781 cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc gctaccaaac
     3841 aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc aatcggcagg
     3901 cggcgctctt tgctgccttt ccccatcacc cacacttcgc cggactccag gtcgaggtgt
     3961 ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat cacttccagc
     4021 attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tatccagcag ccgattcata
     4081 tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga aactcctttc
     4141 gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct acgtagcgca
     4201 gaaagccgta acgccagact tgctgctccc agccctttac ggcgactgcg tacagcaaaa
     4261 ttgcgtacca tcgctgcatc gcattgttgc cagctttgca ggccgttttc gctggcaaaa
     4321 ttgatgatcg cctcaagctg acgctggtag ttaagtaggg ttatcgggct aagctgacgc
     4381 tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt catacgcgtt
     4441 caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt aactgcgttc
     4501 cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacaaca cccaaatcag
     4561 catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc ggtagcacca
     4621 ccagcagctc cggtccgtta agcggcccaa gatagtgctg ttcctgcccc aaacgctgaa
     4681 tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc gacggcgcac
     4741 ctaagcgcca gcgatctgga aacaggcgca gactcgcacc agctaggccg agatcgcgcg
     4801 cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg agactgcgct
     4861 gcaggtagag taggcgataa aacagccctt cgttggcgat agcctgctcc atcaacaacg
     4921 ccatgttctc ttccagaaca tgaatgtgat tacgtgcgcg ggccatgtgc cactcgacca
     4981 acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg cgcgcattgc
     5041 ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca tcaagctccg
     5101 tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc catcgtagac
     5161 atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg cgatatcaag
     5221 acgaccgccg gggagttcca cgcgtacttc ttcgtccagc aaaccttgct gaatccctac
     5281 cgccaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc cacgctcata
     5341 aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat tggcgcgctc
     5401 cggaaaacgc tcgtgacttt ccagaacagg accaagcgtt tctaccgccg cggtatcgac
     5461 atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc ataagattgt
     5521 ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa acggcacggc
     5581 ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat cggtgacggt
     5641 cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg tcagtccttt
     5701 cagacgcaca aaacgggcaa agcagcgcgc accgttaccg cactgcgcca cttcactgcc
     5761 atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg gctcaaccac
     5821 cagcagctgg tcaaacccta cccccaggtg ccgatccgcc aggcgacgaa tcagctccgg
     5881 tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc caaggccatg
     5941 cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg acttttagta
     6001 attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca ccgtggattg
     6061 cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag gcgggaaata
     6121 gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata cagtgagtgc
     6181 cttaaacacg tttttcatta tggattgcct gtaaagttca tcgctttctg ctttctatca
     6241 tcgcaggaga aggcgtaaaa gcaagcgttt agcgactctt tatgaagtct gaaaagtgag
     6301 gaaatgctgc gagtataacg cttactcgtg aggatttatg ttctgctggc agtttcgtga
     6361 tcaacacctc gcgccaggat ggctttatgc actatcgcaa tgtggtttgt ttgttgtctt
     6421 gttcattgtt tttatcgtcg gcatggggat gtcggctttc cgcacctgaa cataatattt
     6481 accagaaaca gggaaaaggc gtggtgtacc tgcgccctta taaagaaaca aatctttctc
     6541 tacctgacgt gaattacaag agtttgcgtc ggttacccaa tttattaata gacccgacaa
     6601 cactagacga atgggataaa gaaccgcctc tgactgattt gacaaccgac tacctctatg
     6661 agggcgcaca ggcctggtat cctcattatt catggcatag cgatggtcgc aatatcctct
     6721 acgcgggtga agttgtgcaa aaccctcccg gcacacctcc cgtcgatgtc gcctcgttca
     6781 aggcatgggg tgattttgcc gcagataaac acagtctcta ttttgaaggc aagcgcaccg
     6841 atgacaatgg tggtggaaat agcttggata tcaaaacgct gcatcaggtg gaattccggc
     6901 ctccctggga tcctgactta ctggggctga tattacgtga cgctaatttc ttgtatatca
     6961 atgggcatcg cctcgctgac cccgagagtt tccgtgtgct ggcgcagaag tcctgggatc
     7021 agcgaggaaa gttttccacc acgtttaacc cctgcattgc cgtaccattt ggcccctggg
     7081 ataccctggc tcgtacacgg accaaaatcc tgctcaacgg cgaacagctt gatgccgacc
     7141 cggacacctt ttccgtcgtg cgctggatgc ccggctcact cttgacctgg cgtgataaaa
     7201 acgggctgca gcgtaaagtc ctcgacaagg aaaatctggc gtgggatgaa gatttaacaa
     7261 agcactgtct ggatttttct ctgctggaaa agaaagtgtt ctggcgtaaa ggacctgctt
     7321 gtaaacagga agaattaccc ggactcgatc cggagcagtt tcaacccatc agtgatgctg
     7381 tcgcccagca tcaggactcg ctttatacca tcatcgaaac agagtctggt aaccgtaagc
     7441 tggagatcgt gaaacttgat gatcccaatc ttattatcaa caaacgtttc aacgccggga
     7501 aacgccacgg ctatttactt acgcgtgccg aagggtagcc ataccattcc ggtttacacg
     7561 tgtttgaatc tgacggaccg ctgatcttac tggataaccg ttctccggat gaacgcgaag
     7621 cccatcttaa tgaccatccc tttttgcgca gatggtatgc ccgcgataac cgctacattt
     7681 acagctttga tggcgcgcag ctctggcgat accgcaccgc tgatccgaaa caagttcgct
     7741 taatctggaa ggaacaacat tcgggatatg tctatggcgt aaattacaaa acgggatatc
     7801 tggacggaaa aattactgat gacggcgaat ttattcctgc cccgcgcaat gaggcgacaa
     7861 aatgaagggt caaataatca cagttgcact catcctgttg ggacacacat ttctgacgcc
     7921 tgctgtacag gccatagggt tcgactatta caatgatcac ggtgttatgt cttacggtaa
     7981 aggttattgg ggggataaga aaatcattgc ccagtttccg gagatgagca gggccggttt
     8041 gcgccttgtg aaaaatatct ctgggaaaac gggttatata ccttccgatg aaataagcat
     8101 agaaaatgag gaataccact gggtgacaga cggccgcgtt attctctggc gtggcaaaat
     8161 cgttagcaat ccaccgggaa cactcactgt cgatattgcc agctttcagg ctatgggccg
     8221 cttcgcggtc gataaatata gcctctattt tgacggacag cgcaccgaaa gtaatagcgg
     8281 cgcgtcacgt gttgagctgg cgacactaaa agctatcgaa ggtaactcta ccacgctggt
     8341 ggatagtaaa aatctctact tgtccggtcg acgtcagggg agcagcagtg atgttactgt
     8401 attagaaaaa agatggtggg gtattaatcc acgtcttatg agtgtaaaca gaaattcata
     8461 ttccaatgat ctgcttatcc gcagtgggca gaatatttat ttaaatggcg ttcaccttac
     8521 ggcgaatgca gactcatttg agataattcg ctggatacct cattcactgc tggtttttcg
     8581 cgacaataag ggtctgcatc gttatccctt tggtcaatta tcaggcaaag cgatacccgt
     8641 agatgatgac gtctcttttg aagtagggga aagtcgcgtt cgctggcgta aacagctcac
     8701 gcccgaccgt cagtggagca agtggataga cctaccagg
//
LOCUS       DAJTQQ010000142         8671 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000142 DAJTQQ010000000
VERSION     DAJTQQ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8671
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.142"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..164
                     /locus_tag="OO309_003698"
     CDS             <1..164
                     /locus_tag="OO309_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR5848685.1"
                     /translation="VFGNPVRGYPSMLVSILFLGGVQLIGIGVLGEYIGRIYLETKSR
                     PRYLIKSRK"
     gene            177..1442
                     /locus_tag="OO309_003699"
     CDS             177..1442
                     /locus_tag="OO309_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HCR5848686.1"
                     /translation="MKIFIMRNWHKISIFILAFTLIWLRRIDILTNAQFWAEDAVFWY
                     KDAYEQGFFSSLITPRNGYFQTVSTLIVGATTFINPIYAPLLSNFFGIIIRAIIIWFL
                     FTDRFKFLSTTSKIFISVYLICMPGLDEVQANITNAHWYLSLYVAMIIISEESKSKLW
                     KAHDLFFIVLSGLSGPFIIFIIASSVFKYLHLSKGVISIRGLYLFYTRLPYLAMILCG
                     LIQATSIILTFNGTRSHAPLGFSFDVMSSIISSNVFLFSFVPWNIANAGWDNHLLSYT
                     LSTLVITLCVFIYIKGNWQMKVFATLPILIVAFSMAKPQLADSVPQLPTLATGNGSRY
                     FVNIHIAIFSLICVCLFQCIKNKTLKIFFKIYVSVILIAMIKLNFFITPLPDMNWSQG
                     AELINKAKHGEAVSFTVLPPWLTLDLIKK"
     gene            complement(1509..2132)
                     /locus_tag="OO309_003700"
     CDS             complement(1509..2132)
                     /locus_tag="OO309_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5848687.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSARY
                     THTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSADRPYWITGANCEVVDIGCVFTETT
                     SKATSASALSVTRSGKINANGSIDVATGVSSVNVVATGVYEVSFSHPLAAVASGYIVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLL"
     gene            complement(2883..3221)
                     /locus_tag="OO309_003701"
                     /pseudo
     CDS             complement(2883..3221)
                     /locus_tag="OO309_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="head-binding protein"
     gene            3286..3744
                     /locus_tag="OO309_003702"
     CDS             3286..3744
                     /locus_tag="OO309_003702"
                     /inference="COORDINATES: protein
                     motif:HMM:NF013043.1,HMM:NF024784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5848688.1"
                     /translation="MEVLYYNKETGIFTWKKKLSARGAVGKVAGTISYGYNAINIDGV
                     RYFAHRLAWLYVYGEWPKQEIDHIDRNRRNNAISNLRDVSRVVNALNVGPRNSNAGIK
                     GVTFCQARNQWQAQINVSGKNITLGRFNTIDEAAIAYKAANMVADHLLSR"
     gene            3876..4136
                     /locus_tag="OO309_003703"
     CDS             3876..4136
                     /locus_tag="OO309_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HCR5848689.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQRAKMNNRSLNSEIIAAIEES
                     LDKQNSASVYIDDAERMAEQQSDMVKKIVFDTLKELYKKDSS"
     gene            complement(4226..6223)
                     /locus_tag="OO309_003704"
     CDS             complement(4226..6223)
                     /locus_tag="OO309_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR5848690.1"
                     /translation="MAKAWKDVIASPQYQALAPEQKAQAQEQYFNEVVAPQAGENAEQ
                     AKQAFYAAYPLPSVQPVETQQPVAQQQPQQSGFMSDLGEAVKETGRGLVQAGVNVANI
                     PASVADAVTSAAAWAGGKLGIGDGTYQPAPRVTTHGLEQDFGLQQGALTPQTTEGRVF
                     AEALPYLTPAGVERAATQAPTLAGRIAQGATRLLAENAVGSLAANSAKDDAEALATDL
                     GVGVLAGGAINAAGRGLGAAYRGVRGAIAPEAQQAIRFAEREGVPLHTTDLLQPTSRV
                     GKMAQTTAENIPLAGTSGMRATQQEARSQLVQRFADKFGEYDPAVVIDSLKAKTSGIR
                     RAAGNRLEQVQNAMAGVNIQPARAIQQIDTEISNLQKLGKVADNETISKLQSYRDELV
                     RNAGPEGPVNLDLKQLSDLRSQFRMDVKGERPVLPNRSDAAIQRVYKAMTDDINGAIG
                     QNLGNDTLRKYQQANAVYADEAAKLKNTRLKNVLMKGDLTPEVVNNMLFSKNKSEIKT
                     LYNSVGRVGRVQMRNGIIGKAMEKSGGSPDQFLRQLNILQNQTGITFKGQDAAYLKGL
                     KNYLQSTQQAAKAAVTTPTGQQTIPFIIGYGTAMNPATTGAAVSYGLLTRAYESEPFR
                     NAMLRMANTPRGSTAFEKAMQQAQKAINALTQGAKSDALSE"
     gene            complement(6223..7518)
                     /locus_tag="OO309_003705"
     CDS             complement(6223..7518)
                     /locus_tag="OO309_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR5848691.1"
                     /translation="MATWQGSNGGLLAGIGGVNSNAPSVNDIGNTLQFIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAKELTRVGVDPNNVA
                     QMYQQNPSGFGEFVDHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGEALT
                     ARGQDIQIRGQNISAQNAALSREIQRAELQEKALDRQIARESNQLKLEELKQKQADVR
                     QKAEIARADRQAAAQGAVDTFSTALDSLNEIEQSPGLSKAVGIRSAFPTVPGSDAANF
                     EARLDTFKAQTFLPMVQSLKGMGALSDAEGKKLSDAVGALSPKMSEKAFRDSIGKIRN
                     QLESKLSTVKKQFDYQEPVQNTPGQQSPAGSNFSSLWGD"
     gene            complement(7528..8220)
                     /locus_tag="OO309_003706"
     CDS             complement(7528..8220)
                     /locus_tag="OO309_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR5848692.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAAIAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            complement(8223..>8671)
                     /locus_tag="OO309_003707"
     CDS             complement(8223..>8671)
                     /locus_tag="OO309_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR5848693.1"
                     /translation="FKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVNVHG
                     QFGGIVYYQEIQPLTFDCHAMYLPEVRGFSKEIGLAFWRYILTNTTVQCVTSFAARKF
                     RHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
BASE COUNT         2254 a         2058 c         1868 g         2491 t
ORIGIN      
        1 ttgtctttgg taacccagta cgcgggtatc cctccatgct tgtatcaata cttttcctgg
       61 gtggagtgca actgatcggg attggtgttc tcggtgaata tataggtaga atttatttag
      121 aaacaaaatc aagaccacgg tacttaatta aaagcagaaa ataacaggat gatcttatga
      181 agatattcat aatgcgcaat tggcacaaga tatcaatttt tatacttgca tttactttga
      241 tttggcttag acgcattgac attttgacaa atgcccagtt ttgggctgag gatgcagttt
      301 tttggtataa agatgcatac gaacaaggtt ttttcagttc actcataacc ccaagaaatg
      361 gttattttca aacagtatca acactcattg ttggtgcgac gacatttatt aatccaattt
      421 acgcaccatt attatcaaac ttttttggaa taataattag agctataatt atatggttct
      481 tatttaccga tagatttaaa ttcctaagca ccacatcaaa aatcttcatc tctgtatatt
      541 taatttgcat gcccgggctt gatgaagtcc aagccaatat aacaaatgct cactggtatt
      601 tatcattgta tgttgcaatg ataataatat ctgaagaatc caagtcaaaa ttatggaaag
      661 cacatgattt attttttata gtattatctg gattgagcgg tccatttatt atattcataa
      721 ttgcatcatc tgtttttaaa tatttacact tatcaaaagg agtaataagc ataagaggtt
      781 tatacttatt ttatactcga ctgccatatt tggcaatgat cttgtgtggg ctgatacagg
      841 caacatcaat aattcttaca tttaatggaa caagatcaca tgctccatta ggattcagtt
      901 ttgatgttat gtcatctata atatcatcta atgtatttct tttttcattt gtcccttgga
      961 atatcgctaa tgctggatgg gacaatcacc ttttatcgta cacactatca actttagtaa
     1021 ttacattatg tgttttcatt tatattaaag gaaattggca aatgaaagtg tttgccaccc
     1081 ttccaatact tattgtcgca ttttctatgg caaaacctca actggcagat tctgttcctc
     1141 aactgccaac acttgcaacc ggaaacgggt caaggtattt tgtaaatatt catattgcaa
     1201 ttttttcatt aatatgcgtt tgtttgtttc aatgcataaa gaataaaacc ttaaaaatat
     1261 tctttaaaat ctatgtttca gtcatcctta ttgcaatgat aaaattaaac ttttttataa
     1321 cccctcttcc tgacatgaac tggtctcaag gagcagaatt aataaataaa gcaaaacatg
     1381 gagaagccgt ctcatttaca gtgctccctc catggttaac tcttgattta ataaaaaaat
     1441 aaaacttcac attaataaat aacaatgaat ccgcctccct acgtattccc gtagggaggc
     1501 tgtcgtgttt atagtagcct ggtaatgcta aatgcaaacg aactagagat tcctgctccg
     1561 ctaagcgtat tgcgcagggt caccctaaat gtatctacgc caatataagt aacatcacaa
     1621 ctaactgaat caggcgcaga tataggtgtt atctgcacaa tataaccgct agcaactgct
     1681 gcgagcggat gagaaaagct aacctcatac acacctgtag caaccacgtt aaccgatgaa
     1741 acaccagtag caacgtcgat actaccattg gcgttaattt ttcccgagcg tgtgactgac
     1801 aatgcagaag ccgatgttgc cttactggtt gtttctgtaa aaacacaccc aatgtcaact
     1861 acctcgcaat tagctcctgt tatccagtat ggtcgatctg cacttggctg ataatcccct
     1921 gcgctctgga acgttgtgcc gttgagtatg actgtaactt ttcccccacc tgagcttgtt
     1981 acttgaatat tagtgtgcgt gtatcttgca ctgctcacgc gatgaaaatt tccaccgttt
     2041 ataaccacag ttacagggcg cgtcccagta ttatctatag caaggtctgc ccccccggca
     2101 ttagcctcaa agtatgggtt gtttaatacc aaagcgcacg aaccgttaag gccatttatg
     2161 gctaattgcg caccacctgt actggaatcc ccctgagtac catttccttc acaagtcagg
     2221 ctattgatag ttgtaccagc accccagcgg tccccgagga taccccattc actgttctga
     2281 ctgcacgtaa gcctatttgc agttatagcg ttcagttcag aatgatttac ggagttggta
     2341 gcacgccaac cgatacgatt actctgcaag tacacgttcg tcatgtcgct cgtgagcatc
     2401 gagtcagtgc gtaacccttc cccgtcaggg tggcggcaga taacaatgtt ttcaagacga
     2461 gtatgtgctt tattctgcac cagcataccg taacttccgg gattcccttt cagcgtcatg
     2521 ttgccgatgt agtcatatgc agccaatctc tgactgttaa acgctgcgtc acctagcatt
     2581 tttacgttaa aatcagtagc tctacattcc agtattgtct cagccatgtt ctcgcctgag
     2641 taaaaacagc ggacacccgg agcacctaca aagtcggcag ggtaatcatc ataattaagt
     2701 accagaggag tggtaacatc atacacccct tgctttattt ttaatgttcc accgaaaggc
     2761 cacgatgtaa gcgggtcttg atatatttca ttttttattg caaatagctc atcttgaaga
     2821 tttttcccgc tattagtgcc aatgtaagta gccccttcat cagattgaag taactgcttt
     2881 aactgatccg ggtcatactt cagcaaattc ggaaaataga actgttgcga accatacgca
     2941 tcatatacag ccattgaatg gccttgctca gttacaaatt tggcaatctg cccgttgtac
     3001 acaggatatc cggcagcatt gataacaatt ggctgagaaa cagggacgtg agatccgtct
     3061 tcattctcta cataaacctg aatctggttt tcaggattta ccgggtcagt gtcaattttc
     3121 ccgatataaa tttttccatt ggcatttgct ttaaacgagc gagctaaagt aaataactga
     3181 cttggttgtg aaacaacaac gttgtataat tgttctggca taataaacat tccggagaaa
     3241 ataatgagta agagacgtca cgaggtagta actcgggagc gccttatgga ggttctctat
     3301 tacaataaag aaacaggaat tttcacttgg aaaaagaaat taagcgccag aggtgctgtt
     3361 gggaaagttg ctggcacaat ttcttatgga tataacgcaa ttaatattga tggcgtaaga
     3421 tattttgcac atagacttgc ctggctgtat gtatatggcg aatggcccaa acaagagatt
     3481 gaccacattg atagaaacag aagaaataac gcaatctcaa accttaggga tgtcagcaga
     3541 gtcgtaaatg cattaaatgt cggacctcga aatagcaatg ctggaatcaa gggggtaact
     3601 ttctgtcaag ccagaaatca atggcaagct cagataaatg tctcaggtaa aaacataacc
     3661 cttgggagat tcaacaccat tgatgaagcg gctatagctt ataaggcagc aaacatggtt
     3721 gctgaccatc tgttgagtag gtgatatctg acatttcatt gctccagacg aatgatatga
     3781 tgcaaccatg atgtgattgc ataccgaaat ggtactattg agtatttatc cagtaagtta
     3841 cgatgccatt ccacccaatt ggtgaggcat caaggatgta cagcaaatac gacgaggcgc
     3901 agtttcactt gagacttccg catgaactcc acgcgaaaat taagcagcgt gcgaagatga
     3961 ataacaggtc tctgaactca gagataattg cagcgattga agaatcactg gataaacaaa
     4021 actctgcatc agtttacatt gacgatgcag agcgtatggc agaacaacaa tccgatatgg
     4081 ttaagaaaat tgtctttgat acgctcaaag agctatataa aaaagacagc agctaaccat
     4141 cagttacgga ggatttatgc aaagagatat gctgaatatt gcgttctaca tatttggttt
     4201 ttgcacgttc ctggtgtttg cgaagctatt ctgacaacgc atcagacttg gcaccctgag
     4261 tcagggcgtt aatggccttt tgcgcctgct gcatggcttt ctcaaacgct gttgatccgc
     4321 gtggggtatt tgccattcgg agcattgcat ttctgaatgg ctcgctctca taggcgcgag
     4381 taagaagtcc gtagcttacc gctgcgccag ttgtcgccgg gttcattgcc gtcccatacc
     4441 cgataatgaa cgggatggtt tgctgccctg ttggtgttgt tactgccgct tttgctgcct
     4501 gctgagtgga ctgaaggtaa tttttcaatc ctttcagata agcagcgtcc tgccccttaa
     4561 atgtgatgcc agtctggttt tgcaggatgt taagctgccg aaggaactgg tcaggggatc
     4621 cgccagattt ctccatcgcc tttccaatga tgccattgcg catttgcacc ctgccaacac
     4681 gaccaactga gttatacagc gtcttaattt ccgatttgtt cttgctgaat agcatgttgt
     4741 tgacaacttc cggcgtcagg tcgcctttca tgagaacatt cttcagcctg gtattcttta
     4801 gtttcgccgc ttcgtcagcg tagacggcat tggcctgctg atatttacgg agagtatcgt
     4861 tgccaagatt ctgaccaatg gcaccattga tatcgtcggt catcgccttg taaacgcgct
     4921 gaatggcagc atcggaacgg tttggtaaca ctggtcgctc acccttcacg tccattctga
     4981 actgactgcg caggtcgctt aattgcttca aatccagatt taccggacct tcagggccag
     5041 cattgcgaac aagctcatca cgatatgact gaagttttga aattgtctcg ttatcagcca
     5101 ccttaccaag cttctgcagg ttagatattt ctgtatcaat ctgctgaatt gctcgcgcag
     5161 gctgaatgtt tactccagcc atagcattct gaacctgctc cagtcgatta ccggcggcac
     5221 gacgaattcc tgatgttttc gctttaaggc tgtcaataac aaccgctgga tcatactccc
     5281 cgaatttatc agcaaatctc tgcaccaact ggcttctcgc ttcctgttgc gttgctctca
     5341 ttccgcttgt gccagccaga gggatatttt ctgctgtagt ctgcgccatt tttccgacgc
     5401 gggaagttgg ttgtaacagg tctgtggtgt gcagaggaac tccttcacgc tctgcaaatc
     5461 tgatagcctg ctgagcttct ggcgcgatag caccacgaac gccacgataa gcagcaccta
     5521 atccacgtcc ggcagcgtta atagcaccgc cagcaagtac accaacgcct aaatcggtgg
     5581 cgagtgcttc cgcatcatct ttcgcactat ttgcagcaag tgatccaact gcgttttcag
     5641 cgagaaggcg agttgcaccc tgagcaattc gaccagcaag tgttggtgcc tgtgttgccg
     5701 ctctctcaac gccggcagga gtgaggtaag gcaatgcttc agcaaatacc ctgccctctg
     5761 tcgtttgtgg agtcagcgca ccttgctgaa ggccaaagtc ctgctctaat ccatgcgttg
     5821 ttactcgtgg cgctggttga tatgttccat cgccaatgcc gagtttaccg ccagcccatg
     5881 cagccgcgct tgttacagca tcggcaaccg atgcaggtat gtttgccacg ttcacgccag
     5941 cctgcaccag tccgcgacca gtctctttta ctgcttcgcc aagatcagac ataaatccac
     6001 tttgctgtgg ttgttgctgt gctactggtt gctgtgtctc cactggctgc acagatggca
     6061 atggataggc agcatagaaa gcttgcttag cctgctctgc attttctccg gcttgcgggg
     6121 caacgacttc attgaagtat tgctcctgag cctgcgcttt ttgttctggt gctaacgcct
     6181 gatactgtgg agatgcgata acatctttcc atgctttagc cattaatcac cccatagtga
     6241 agaaaaatta ctgccagcag gagattgttg ccctggcgta ttctgcaccg gctcctggta
     6301 atcaaactgt tttttaacag tgctcaactt gctttcaagc tgatttcgaa tctttccgat
     6361 agagtcacga aaagcctttt cactcatttt gggacttaga gcaccaaccg catcggataa
     6421 ttttttaccc tcagcatctg aaagagcccc catccccttc agggactgca ccatagggag
     6481 gaaagtttga gctttaaagg tgtcgagtct tgcttcaaag ttagccgcat cagatccagg
     6541 aactgtcgga aacgctgagc gaataccaac tgcttttgaa agtccagggc tttgctctat
     6601 ctcgttgaga gaatcaagcg cagtgctgaa cgtatcaaca gcgccctgag cggcggcctg
     6661 tctgtcagca cgagctattt cagccttttg ccgaacatct gcctgtttct gctttagctc
     6721 ttcaagcttt aactgattgc tttctctggc tatctgtctg tccagagcct tttcttgtaa
     6781 ttctgctctt tgtatttcgc gggaaagagc agcattctgt gcgctgatgt tctggccacg
     6841 tatctggatg tcctgacctc gtgctgttag tgcctcgcca gcctgattgc tgcggattgt
     6901 ctctgccagc ctacctcggt caatctcacg accagccatc ttgtcctgaa cattgaagta
     6961 atcaatcgga ccaagagcag ccatcccaag gtgatcaaca aactcaccaa atcctgaagg
     7021 attctgctga tacatctgag caacgttgtt aggatcaacg ccgacgcgag tcagttcctt
     7081 ggcgttgttt tgcagccatg attgcattgc ttctggagac gatgacgcaa ggcgtgcgcc
     7141 agccgctaag gtgccgatag aattacgctg ctcttcatca atgaatccca tgcctttacg
     7201 aacggattca atctggtctg gatattgagt agccaactga cgcaaagcac cgcgatcacc
     7261 agacgcataa gcattagcgt atgcctgctg aaattctttc tgccgctgag cctgcttttc
     7321 ctgctgaaac acccccgcaa tacctgaaag gccttgcaaa gcagtcagcc caacattgtt
     7381 agcgcctgaa cgctcaatat cattgttctg cctgataaac tgaagcgtat tgccgatgtc
     7441 atttacgctc ggagcgtttg agtttacgcc gccgatacca gccaacaatc caccatttga
     7501 tccttgccaa gtagccatga ttacccctta aaacaacgag ccaagcaatc cgataccagc
     7561 accaatgcca gcgccccaag gtgttgatgt tcccaaaagg ctggcaagac ctgcaccggc
     7621 aatcgcacca gacgtgccac cgctaattgc agtctgaaga cttgatggtt tattggcatt
     7681 agcagcggca attgctgcac tttgctgtgc aatgctgctc atgttgttgg cgtatgtctg
     7741 cccagcgttt gcctgacctt gcagcgcacc aagcccaacg tttgccagat tattgtaatt
     7801 gctcatctgg tttgataacc acgactgacc gagagtcgga gcaatcgtag ccagttgatt
     7861 gcttgtggct gtcgaaccaa gtcctcccgt agcctccgca gcagcaagac tctggtaacg
     7921 agcctgacct gcaaggtctt tatactgctg agagttgtaa tactgattaa gtgcctgacc
     7981 ctgtccttct aaactggaaa ggttctgaag ctggttaaca tactgctccg caagcggcgt
     8041 gaacggagca aggtttttca tgatcgtctg ccactgctga ttttgcaggt ctgcggcata
     8101 cttctgagct tctgctgcat actttgcact tttatcagag ctgccacctt tcccgccttt
     8161 ttcagggcaa taaggttcct cgccgcgcag ttttctgccc agcttaaatg catataacat
     8221 ggctatctcc cgtgattcag gaagtcgatt agttcttcgc gtgtagcact gtaaaatgtc
     8281 acgtcatcca cgcctttgaa gtatttcttg atggttccta ctcgcttaag gccaatcatt
     8341 gcgcagtaca tctgaccgtg gcggaatttg cgcgcagcga acgatgtgac gcactgaacg
     8401 gtggtgttag tcagaatgta tcgccagaac gccagcccga tttccttgct gaatccacga
     8461 acctctggca ggtacatggc gtggcaatca aaggtcaaag gctgaatctc ctgatagtaa
     8521 acaatgccgc cgaactgccc gtgcacgttc acctcgaagt aacggcattc aggcttgtag
     8581 tcgtatccat caccgttgtt gctcccggcg ataatgtcgg ggtgatttcc cacggcttct
     8641 atcaggtcga tgtttcgcgt tggtttgaac t
//
LOCUS       DAJTQQ010000143         8623 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000143 DAJTQQ010000000
VERSION     DAJTQQ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8623
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.143"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(769..1965)
                     /gene="hemY"
                     /locus_tag="OO309_003708"
     CDS             complement(769..1965)
                     /gene="hemY"
                     /locus_tag="OO309_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR5848694.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1968..3161)
                     /gene="hemX"
                     /locus_tag="OO309_003709"
     CDS             complement(1968..3161)
                     /gene="hemX"
                     /locus_tag="OO309_003709"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5848695.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPQGDTPAA
                     APQGE"
     gene            complement(3183..3923)
                     /gene="hemD"
                     /locus_tag="OO309_003710"
     CDS             complement(3183..3923)
                     /gene="hemD"
                     /locus_tag="OO309_003710"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR5848696.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3920..4861)
                     /gene="hemC"
                     /locus_tag="OO309_003711"
     CDS             complement(3920..4861)
                     /gene="hemC"
                     /locus_tag="OO309_003711"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR5848697.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5248..7794
                     /gene="cyaA"
                     /locus_tag="OO309_003712"
     CDS             5248..7794
                     /gene="cyaA"
                     /locus_tag="OO309_003712"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR5848698.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7834..8154)
                     /gene="cyaY"
                     /locus_tag="OO309_003713"
     CDS             complement(7834..8154)
                     /gene="cyaY"
                     /locus_tag="OO309_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR5848699.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8202..>8623)
                     /locus_tag="OO309_003714"
     CDS             complement(8202..>8623)
                     /locus_tag="OO309_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5848700.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1964 a         2231 c         2214 g         2214 t
ORIGIN      
        1 agttgacggg gtatctgctg cgctggccat gagaggaaga gcggcggcga cagcaacaac
       61 aagacgtttg ggtgaaaacg aaaattccat gcaaaatgct ccggtttcat gtcatcaaaa
      121 tgatgacgta attaagcatt gataattgag atccctctcc ctgacaggat gattacataa
      181 ataatagtga caaaaataaa ttatttattt atccagaaaa tgaattggaa aatcaggaga
      241 gcgttttcaa tcctacctct ggcgcagttg atatgtaagg caggtttatt atatcgcgtt
      301 gattattgat gctgttttta gttttaacgg caattaatat atgtgttatt aattgaatga
      361 attttatcat tcataataag tatgtgtagg atcaagctca ggttaaatat tcactcagga
      421 agttattact caggaagcaa agaggattac agaattatct cataacaagt gttaagggat
      481 gttatttccc gattctctgt ggcataataa acgagtagat gctcattcca tctcttatgt
      541 tcgccttagt gcctcataaa ctccggaatg acgcagagcc gtttacggtg cttatcgtcc
      601 actgacagat gtcgcttatg cctcatcaga caccatggac acaacgttga gtgaagcacc
      661 cacttgttgt catacagacc tgttttaacg cctgctccgt aataagagca ggcgtttttt
      721 tatgcatcag aaaggtcccg gaggtgcttt cctccgggtg agaaggaact actgtggcgg
      781 gttattctgc aacgttaaca tcaaaccgtc gcgacgcatc gctgcagctt cttccggctt
      841 gtgcagtctg tccagcgcgt cggcaagcca tgcgtaatcg taggcgtccg gacgttgttt
      901 cagcgccgcg cggaaggcga gcgatgcttc ctgccattca ccgtgcttca tcagtgactg
      961 acccaatgtg ctccacaaca gcgggcgatc accgacgttt ttgatttgct ggcgtagcac
     1021 tttttccagc tgttccgggt tattggtttt cagacgcggg atcggcagca gtaggcgatc
     1081 gtcatactgg cgtttcaggc cgtcgatgat aatttgctgg gcagtatcat gatcgtcaca
     1141 ttcaataaga tgttccgcca ttgccacctg caacgctacc tgatgacgcg ttttccggct
     1201 ttggtttttc caccagttac gcaaaccttc actaccgtta tcggcacgcg cctgatccat
     1261 caggccaatc catgcctgtt gttccagcat tgcacgatgt tcttcatcac caacatgggc
     1321 tttcgccatt gatgggatga tatccagcag agaactccat gcccctgtgc ggatatacgc
     1381 ctgttccgcc agacgtaata cttcaggatg gcgtggcgta acttccagca gcttatccac
     1441 gccgtggcgt gcagcatggt tttcattacg ggccagttgc agacgtacac gggtgatttc
     1501 taccggaatg gtgtcattgc cggccagctc cgctgcgcgt tccagatgtt ggttggcgcg
     1561 tgcttcatca ccacgttgtt gcgcggcttc ggcagccagt agatagttca ccaccggttg
     1621 ttcagcgtga tcggcatttt tcgccatcag cttttcaact tgctgataat caccttccgc
     1681 cagtttcagc agcgcctgtt cggtctgctt acgcgcacgg cgacgcttac gtccgacaaa
     1741 ccatccacgg gtgtgcgcgc cagtgcggaa gatccgccgc agtagccact caatggcaaa
     1801 cagcactacc atcgccagaa tcaatatgat cgccaggccc gtgacgctgg tttcgatatt
     1861 gtagttgtcg gtctggatca gcacataacc ctgatgaccg gcaatcatcg ggccaaccac
     1921 tatccccgca atcagcaaaa caaagagtaa taacactttt agcatggtta ttctccttgc
     1981 ggcgcggctg ccggagtatc accttgcggt gcaggtgcag gtgcaggtgc aggtttagct
     2041 tccgttgtcc ccgctgccgg ttgtgccagc aggttacgca cgcgagtctg catcagtttt
     2101 tccagcatcg cctggctttg cagggtttcc ggaagatcca tcgagatatt ttgctggctt
     2161 aactggtcca cctcgtcgag gaacgctttg gtggtggcat catcagtatc gtagtaagca
     2221 cgtacccagg tggagacgtt ctccagcgcc tggcgataag tctcttcctg atgacgcggt
     2281 acagcttgtg ctgcgaccag caggcgagag cgaatatttt cgcgcagata gatatcctga
     2341 tttggcgcta acagcggtac ggcggtgtca tcacgacggc gaatcgtaat gaagttgtcc
     2401 ataaagttct gccagctttt ttgcagattg atacgccatt cgctgatgga actggaaagc
     2461 tcttcaccgt ctgaatccat cggcgaacca tcgctgtcat tatcggccag acgcaggtta
     2521 tctacctgat ttgaaagctg attaagctta aggatgatgc cgtcataatc cacctgcgat
     2581 actgcagaaa ggctggcgat atcatcggta attgcccgac gaacggtaat cagactcggg
     2641 tcattcatat ccgccaggct ggcgtctgca cttttcagca acgctgcaga ggtcgtgacg
     2701 tcctgatcgc tccacagctt ccgtccggcg agtttcacca gaaaatcggc ctgagccagc
     2761 agccaggttt tagcatcgct gccggaaatg gtggcgatct tttgttggac ttcatccaac
     2821 tgttttgcca gcgtttcttg ctgacgattc gcctgcttaa gctgcgcagc ttgttgctta
     2881 ataatgcctt ccagctcggc tttttggctc tcctgggctt tttgcaatgc cgtcagttgg
     2941 ttagccaggg catcgctggt ggcggtctga ttgacggcct gttgtttacc ccagccatac
     3001 aaaccgacac ccgccgccag agcaatagcg atagccaccg cgctgagaat caatgcagta
     3061 ttgttcttac tctttttttc tgttgcgaca ggttgtgacg tggtgtccac ggcctccctg
     3121 gtctcttcaa ccacggcgga ggttttttct tgttccgtca ttatggcttc ctgttatgag
     3181 agttattgta atgcccgtaa aagcgcatcg ttgtcagcgt tatcggcgac cttaatgtct
     3241 tgccagccca gttcccgggc gagtttcgcc aaacgctcac tgacgaccaa tagtcgacag
     3301 tgtagtaacc agtgctcacg ataccattgt gggatcagcg accagagttg ctgcaacatt
     3361 tcaccgctgg taacaacgac catcgtcacc tcgcgggatt gccagcgcat cgcttcttct
     3421 gcaccatcgt aatggattgc gcatcgttga taacattcac aaaaagtgac ctcagcaccg
     3481 cgcaccgtca gggtatcccc aattagctca cgaccgccat tgccacgtaa tatcagcgca
     3541 cgtttgcccg caatattttg taattcaggt aattgtagca agacttcgct gatttcccga
     3601 tcctgtgggt agagaatctt ctgtccgctt acggtatgta gcgccagtgc ggtggtgcgt
     3661 ccaatggcga aataagtagg tagttggggc catttacgat cttgctgatg cagctgtgat
     3721 tgggcaaaag caaccgcgtg ttgcgagagg gcaaacaaca gatcgctctc ccccagcgct
     3781 gccagttgat cagcaagttg cggtaattgt cgacccggag aaaactcaat cagcggaaaa
     3841 tgccaggcca cctgccccag tgtgcgcaga cggctcacta actcttctcc agcgggagac
     3901 gggcgggtga ccaggatact catgccgggg cctctccgtt atagacttca gcgaggatct
     3961 cgcgcgcgcc gttattcagt agctcttctg ccagcgaaat ccccatttgt tcggcatctt
     4021 gcggcgcacc gcggcgttca ccgcgaataa tctgcgaacc gtccggcgcg ccgaccagcg
     4081 cacatagcca gatttcgcca tcaataagct cggcgtagct accaattggc acctgacatc
     4141 cgccttcgag acgggtattc atggcgcgtt ctgcggtaac gcgcagtgca gtttcgtggt
     4201 gattcagcgc ggcaagcagc tcgcgagtgc gtgtatcatc aaggcggcat tcaataccga
     4261 ctgcaccttg tcctaccgcc ggaagagaaa tctcgggtgg taacgcggcg cgaatgcggg
     4321 actccagacc taaacgtttt agtccggcta cggcaagaat gatggcatcg tattcgccgt
     4381 tatctagttt gctcaggcga gtgccgacgt tgccgcgcag ggagcggata atcagatccg
     4441 ggcggcgttc agccagttgg cactggcgac gtaaactgga cgtcccgacg atactgcctg
     4501 ctggtaacgc atccagactg tcatagttat tggacacaaa ggcatcgcga ggatcttcac
     4561 gctcgcaaat agtgaccagc cccagacctt gcgggaattc aaccggcaca tctttcattg
     4621 aatgtacggc gatatcagcg cgattctcca ggagcgcgac ttccagctct tttacaaata
     4681 agccttttcc gcctactttc gccagcggcg tatcaagaat cacatcgccg cgcgtcacca
     4741 tcggtaccag ttcaacgacc aggcccggat ggctcgccat caacttgtct ttgacatagt
     4801 gtgcctgcca gagtgcaagt gggctttggc gtgtggcaat tcttaaaaca ttgtctaaca
     4861 tgcttgttac cgtcattatc atccgtggtc catcctaaca tccttgccag agtgatgtca
     4921 gtgttgtggt gaaacgtaga cgcctgcgca aaccgtaaaa tgaggtctgg cagtggatcc
     4981 tgacaggcgt ttcacgccgt cgtaataagg aatttacaga gaataaacgg tgctacactt
     5041 gtatgtagcg catctttctt tacggtcaat cagcaaggtg ttaaattgat cacgttttag
     5101 accatttttt cgtcgtgaaa ctaaaaaaac caggcgcgaa aagtggtaac ggttaccttt
     5161 gacatacgaa atatcccgaa tgccgcgtgt taccgttgat gttggcggaa tcacagtcat
     5221 gacgggtagc aaatcaggcg atacgtcttg tacctctata ttgagactct gaaacagaga
     5281 ctggatgcca taaatcaatt gcgtgtggat cgcgcgcttg ctgctatggg gcctgcattc
     5341 caacaggtct acagtctact gccgacattg ttgcactatc accatccgcg aatgccgggt
     5401 taccttgatg gtaacgttcc caaaggcatt tgcctttaca cgcctgatga aactcaacgc
     5461 cactacctga acgagcttga actgtatcgt ggaatgtcag tacaggatcc accgaaaggt
     5521 gagcttccaa ttactggtgt atacaccatg ggcagcacct cgtccgtagg gcaaagttgt
     5581 tcctctgacc tggatatctg ggtctgtcat caatcctggc tcgatagcga agagcgccag
     5641 ttactacaac gtaaatgcag cctgctggaa agctgggccg cctcgctggg tgtggaagtc
     5701 agcttcttcc tgattgatga aaaccgcttc cgtcataatg aaagcggcag cctggggggc
     5761 gaagattgtg gctccaccca gcatatactg ctgcttgacg aattttatcg tactgccgtg
     5821 cgtctcgccg gtaagcgtat tctgtggaat atggtgccgt gcgacgaaga agagcattac
     5881 gacgactatg tgatgacgct ttacgcgcag ggcgtgctga cgccaaatga atggctggat
     5941 ctcggtggct taagctcgct ttctgctgaa gagtactttg gtgccagcct ttggcagctc
     6001 tacaagagta tcgattcccc atacaaagcg gtgctgaaaa cactgctgct ggaagcctat
     6061 tcctgggaat acccgaaccc acgtctgctg gcgaaagata tcaaacagcg tttgcacgac
     6121 ggcgagattg tatcgtttgg cctcgatcca tactgcatga tgctggagcg tgttactgaa
     6181 tacctgacgg cgattgaaga tttcacccgt ctggatttag tacgtcgctg cttctattta
     6241 aaagtgtgcg aaaaactcag ccgtgaacgc gcctgtgtag gctggcgtcg cgcagtgttg
     6301 agccagttag tgagcgagtg gggttgggac gaagctcgtc tggcaatgct cgataaccgt
     6361 gctaactgga agattgatca ggtgcgtgag gcgcacaacg agctgctcga cgcgatgatg
     6421 cagagctacc gtaatctgat ccgctttgcg cgtcgcaata accttagcgt ctccgccagt
     6481 ccgcaggata tcggcgtact gacgcgtaaa ctgtatgccg cgtttgaagc attgccaggt
     6541 aaagtgacgc tggtaaaccc gcagatttca cccgatctct cggaaccgaa tctgaccttt
     6601 atttatgtgc cgccgggccg ggctaaccgt tcaggttggt atctgtataa ccgcgcgcca
     6661 aatattgagt cgatcatcag ccatcagccg ctggaatata accgttacct gaataaactg
     6721 gtggcgtggg catggtttaa cggcctgctg acctcgcgca cccgtttgta tattaaaggt
     6781 aacggcattg tcgatttgcc taagttgcag gagatggtcg ccgacgtgtc gcaccatttc
     6841 ccgctgcgct tacctgcacc gacaccgaag gcgctctaca gcccgtgtga gattcgccat
     6901 ctggcgatta tcgttaacct ggaatatgac ccgacagctg cgttccgcaa tcaggtggtg
     6961 cattttgatt tccgtaagct ggatgtcttc agctttggcg agaatcaaaa ttgcctggta
     7021 ggtagcgttg acctgctgta ccgcaactcg tggaacgaag tgcgtacgct gcacttcaac
     7081 ggcgagcaat cgatgatcga agccctgaaa actattctcg gcaaaatgca tcaggacgcc
     7141 gcaccgccag atagcgtgga agtcttctgt tatagccagc atctgcgcgg cttaatccgt
     7201 actcgcgtgc agcaactggt ttctgagtgt attgaactgc gcctttccag cacccgccag
     7261 gaaaccgggc gtttcaaggc gctgcgcgtt tctggtcaaa cctggggctt gttcttcgaa
     7321 cgcctgaatg tatcggtaca gaagctggag aacgccatcg agttttatgg cgctatttcg
     7381 cataacaaac tgcacggctt gtcagtgcag gttgaaacca atcacgtcaa attaccggcg
     7441 gtggtggacg gctttgccag cgaagggatc atccagttct ttttcgaaga aacgcaagac
     7501 gagaatggct ttaatatcta cattctcgac gaaagcaacc gggttgaggt gtatcaccac
     7561 tgcgaaggca gcaaagagga gctggtacgt gacgtcagtc gcttctactc gtcatcgcac
     7621 gaccgcttca cctacggctc aagcttcatc aacttcaacc tgccgcagtt ctatcagatt
     7681 gtgaaggttg atggtcgcga acaggtgatt ccgttccgaa caaaatcgat tggcaacctg
     7741 ccgcccgcca atcaggatca cgatacgccg ctattacagc agtatttttc gtgatgagcg
     7801 tgccggaaag cgaggcttat ccggcatgca atcttagcgg aaactgactg tttcacccgc
     7861 ctgttgcgtc gccgcctgtt ccagcaaatc ccagaaggtt tcgccgctgc gatcgcaaat
     7921 ccattcatcg cctttcaggt caaaatggta gccgccctgt ttggttgcca gccacacctg
     7981 gtgcagcggc tcctggcggt taataatgat tttgctgccg ttttcaaagg taatggtcag
     8041 tacgccgccg ttgatttcgc agtcgatatc gctgtcgcca tccctgtcgt ccagacgctc
     8101 ttcaatggtc agccacagtt gatcagccag gcgatgaaat tcactgtcgt tcattgttgt
     8161 atcctgtttt taagtgatgg cggcagtata gcggcatggg gtcagggctt caaagtttgc
     8221 acctctgctg ctgcgttccg gcatgattca tccgtcaccg gaataatgat gtctctgttt
     8281 tgaacttcac gtacgttctc ctctctgaca cgagttttcg ccgggcaggg ggagggcgta
     8341 atccttacct ctgtatcggt ccatgtataa acattgcgat cgtcataagc gaagcgatcg
     8401 tttttagtta gcgaaccgtt aactggaata atttgcaagc tctttgaccg taattggcgg
     8461 ataaagtaag catgctgttt gccgctatta aatgagcgat caatgaccag gtctggcgta
     8521 tccagcggga ttatctcaag ctggtcttca ccaaatgtcg ataattttgt tacatacagc
     8581 cgatctttat attgcgcaat attgccagta atcagatgga att
//
LOCUS       DAJTQQ010000144         8556 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000144 DAJTQQ010000000
VERSION     DAJTQQ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.144"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..177
                     /gene="csgC"
                     /locus_tag="OO309_003715"
     CDS             <1..177
                     /gene="csgC"
                     /locus_tag="OO309_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR5848701.1"
                     /translation="QSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQA
                     LHLSQQWTPSSEKS"
     gene            298..609
                     /locus_tag="OO309_003716"
     CDS             298..609
                     /locus_tag="OO309_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5848702.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            704..1237
                     /gene="ymdB"
                     /locus_tag="OO309_003717"
     CDS             704..1237
                     /gene="ymdB"
                     /locus_tag="OO309_003717"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR5848703.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1179..2660
                     /gene="clsC"
                     /locus_tag="OO309_003718"
     CDS             1179..2660
                     /gene="clsC"
                     /locus_tag="OO309_003718"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR5848704.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2668..3825)
                     /gene="mdoC"
                     /locus_tag="OO309_003719"
     CDS             complement(2668..3825)
                     /gene="mdoC"
                     /locus_tag="OO309_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR5848705.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4219..5754
                     /gene="mdoG"
                     /locus_tag="OO309_003720"
     CDS             4219..5754
                     /gene="mdoG"
                     /locus_tag="OO309_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR5848706.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5747..8290
                     /gene="mdoH"
                     /locus_tag="OO309_003721"
     CDS             5747..8290
                     /gene="mdoH"
                     /locus_tag="OO309_003721"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR5848707.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2171 a         2029 c         2221 g         2135 t
ORIGIN      
        1 caaagtcaga cgaagcaaga aaagaccctc tcattgcctg ctaatcaacc cattgctttg
       61 acgaagttga gtttaaatat ttccccggaa gatcgggtga aaatagttgt tactgtttct
      121 gatggacagg cacttcattt atcacaacaa tggacgccct cttcagaaaa gtcttaattt
      181 gttgaaatat cgagcataag atgaatctgg agagaatggt ctgctgcgaa tcagccaacc
      241 tgaaagtatg gataacacaa ccttcaagga tgactaatca ttgaggaaat agaataaatg
      301 ttcagacctt ttttagactc tcttatgctc ggcagtttgt tttttccttt tattgccatt
      361 gcaggaagca ccgcgcaagg gggcgtgatc catttttatg gccaaattgt ggaaccggca
      421 tgtgacgtca gcacccagtc atcacccgta gaaatgaact gcccacagaa tggttctatt
      481 ccgggcaaaa cctactccag caaagcgtta atgagcggca atgtcaaaaa cgcgcaaata
      541 gcctcagtaa aagtgcagta tcttgataaa cagaaaaagc tggctgtaat gaacatcgaa
      601 tataactaag tttctgaaca actcacgttg ctgagcagaa aaatgcgatt taaccaaaaa
      661 gcctgctgta cacttaagaa acaagaaggt gtaaggaggc gttatgaaaa cgcgtattca
      721 tgttgtgcag ggtgatatta ccaaactggc cgttgatgtg attgtgaatg cggctaatcc
      781 gtcattaatg ggaggcggcg gcgtcgatgg ggccattcat cgcgcagcgg gtccggccct
      841 gctggatgcc tgtttaaaag tcaggcaaca gcagggcgat tgccctacgg ggcatgccgt
      901 tattacgctt gcaggcgatc ttcccgctaa agccgtagta cacaccgtcg ggccagtctg
      961 gcgtggtggg gaacaaaacg aagaccagct tttgcaggat gcctatctga atagcctacg
     1021 actggtggcg gcaaacagct atacgtcagt ggcttttcct gcaatcagta ctggggttta
     1081 tagttaccct cgtgcggcag cggctgaaat cgcagttaaa accgtttcag aatttattac
     1141 ccgtcacgct ttacccgaac aggtatactt tgtctgttat gatgaagaaa acgcccacct
     1201 ctacgaaaga ctccttaccc aacaaggaga tgaatgattt gccccggctg gcgagcgcgg
     1261 tgctgccact gtgttcgcaa catcccggtc agtgtggtct tttccctctg gagaaaagtc
     1321 tggatgcgtt tgccgcccgg tatcgtctgg ccgaaatggc agagcatacg ctggatgttc
     1381 agtattacat ctggcaggac gatatgtcgg ggcggttact gttttccgcc ctgttagccg
     1441 cagcaaagcg tggcgttcgc gtccgtttgt tgctggacga caacaatacg cccggacttg
     1501 acgacatttt gcgcttgctt gacagtcatc cacgcattga agtccggctt tttaatcctt
     1561 tctcgtttcg cttgctacgt ccgcttggat atatcaccga cttttcccgt ctcaatcgcc
     1621 ggatgcacaa taaaagtttc actgtcgatg gcgtggttac cctagtggga ggacgaaata
     1681 ttggtgatgc ctattttggt gcaggggaag agccactttt ttcggattta gatgtcatgg
     1741 caataggccc cgtggtagag gatgttgccg atgatttcgc ccgctactgg tattgcaaat
     1801 cggtttcacc cttacagcag gtgctggatg tcccggaagg tgaaatggcg gatcgcatcg
     1861 agttacccgc ctcctggcat aacgatgcca tgacgcatcg ttatttacgc aaaatggaat
     1921 ccagtccatt tataaatcat ctggttgatg gaacgttgcc gcttatctgg gcgaagacac
     1981 gtttattaag tgatgatccg gcgaaagggg agggcaaggc aaaacggcat tcactgttac
     2041 cgcagcgcct gttcgatatc atgggctcac ccagtgaacg catcgatatt atctcttcct
     2101 attttgtacc gacacgcgca ggtgtggcgc aactcttacg gatggtgaga aaaggggtaa
     2161 agattgcgat cctaaccaat tctcttgccg ctaacgatgt tgctgtcgtc catgccggat
     2221 acgcgcgctg gcgcaaaaaa ttgctccgct atggcgtgga attatatgaa ctcaagccga
     2281 cgcgtgaaca aagcagtacg ttacacgatc gcggcataac cggtaattcc ggtgccagcc
     2341 tgcatgctaa aacttttagc atcgatggta aaacggtgtt tatcggctct ttcaatttcg
     2401 atccgcgttc aacattgctc aatactgaaa tgggcttcgt gatagagagc gaaacgctgg
     2461 cacagttaat tgataaacgc tttattcaga gccagtacga tgcggcctgg cagctccgtc
     2521 tggacaggtg gggacggatc aactgggttg atcgtcatgc aaagaaagag gttattctca
     2581 aaaaagaacc cgccaccagt ttctggaagc gggttatggt cagactggcg tcgatattgc
     2641 ccgtggaatg gttattgtaa atgtggctta acgggctggt gctttatcgt tctcacgctt
     2701 aacaaccggt ttaccagaga acaaaaactt cagtaacggg atgcgtaaat gaatttcata
     2761 cagaattatc gcaatcccta ctacgaatat caggccacag agaaaaccaa gccagttgga
     2821 ggtgatgtgc ggtgtaatgt atgcgccgaa aaacagcgtt aacgggtggt gaaccagata
     2881 gataaacagc gatgcgttaa caaaataagt cacccgcgct gactggaagt taagcaaacg
     2941 gtggccaaag gagaagacca cattcaccat ccacaggccg aggaccatgg tgatcaccga
     3001 ctcggtttcg tacatccagg catcgccact gccatagcgc tggttgagta aataagcgac
     3061 aaacgccaat gctgcagcga gggtacagcc acgagacggc gtggtaaaca aggctttaag
     3121 atgagggaaa atgaaagcca gtgcgccgag gataaagaac ggtaaataaa atagcgtttg
     3181 catgacaata aaattgaaca tgccattact cagaatgggt ggatacacaa taaaaatcgt
     3241 tcttcttatt accgcatagc cgatgccgag gcataaaaaa atcaccgata gttttaccat
     3301 cgagaatttt ttattcgttt tatcagaatt ttctaaatta tttctgatgc gcttaaatat
     3361 ccatacacac agcgtcgtca tgactactaa caccagtaaa aaccacaggt gtgatattaa
     3421 ttcccaggcc aacgtattat atttgtcata caatgatagc ccaggccaac tttccgcttt
     3481 ccctttgaca tattgcagca taataaattg cggtaatgtc agtaggggga tggctgttaa
     3541 catcgggata cctacacgtt cgacacgtac tttccaccat tttttcaagg gatagcgtaa
     3601 aaaaagcatg taggaaaagt atccggatat aacgaaaaat acctgcatgc ggaacgagtg
     3661 gatgaagtca ttaaaaaggg ttagccacaa tgacggttcg gcgctattca catgccatgt
     3721 atggctcgaa tagattaaag aaatatgaaa agggatccct aacaacatca gccaggcgcg
     3781 gatggagtcg aggaaatatt cacgttgcgc gggtactgga ttcatatatg gttaactaat
     3841 ctcggatttt tcgtcttatc cctgtcgggt tatgccttta ggcttgttgc catagcgaca
     3901 ccgacctgac cgcgccaggc gcaggcttca aggtttttat gcatagcatc atcgctacca
     3961 ctaaccagaa tggaagcgtc tgtaagacgg ttgataaata aatttgctgg caaaccctac
     4021 acgaagtcga tgcttctgtc tttaggagaa gcacggaaag tgaaaacggt tgcaatcagg
     4081 tgcttaatcc atgagccagc gtgctgaacg ataccgggat tcagttgtcg gaatggctgg
     4141 ttatccatta aaatagatcg gatcgatata agcacacaaa gggggaagtg cttactaatt
     4201 atgaaacata aactacaaat gatgaaaatg cgttggttga gtgctgcagt aatgttaacc
     4261 ctgtatacat cttcaagctg ggctttcagt attgatgatg tcgcaaagca agctcaatcc
     4321 ttagccggga aaggctatga ggcgcctaaa agcaacttgc cctccgtttt ccgcgacatg
     4381 aaatacgcgg actatcagca gatccagttt aatcatgaca aagcgtactg gaacaatctg
     4441 aagaccccat tcaaactcga gttctaccat cagggtatgt acttcgatac cccggtcaaa
     4501 ataaatgaag tgactgccac cgcagtcaaa cgaatcaaat acagcccgga ttatttcact
     4561 ttcggcgatg ttcagcatga caaagacacg gtaaaagacc ttggttttgc cggttttaaa
     4621 gtgctttacc cgatcaacag caaagataaa aacgatgaaa tcgtcagcat gctcggggcc
     4681 agctatttcc gcgtgattgg tgcaggtcag gtttatggcc tttctgcccg cggcctagca
     4741 attgataccg ctttgccatc gggtgaagaa tttccacgct tcaaagagtt ctggatcgag
     4801 cgtccaaaac cgactgataa acgtttaacc atttatgcat tgcttgactc gccgcgtgcg
     4861 acaggtgctt acaaattcgt ggttatgcca gggcgtgaca cggttgtgga tgtgcagtcg
     4921 aaaatctatc tgcgcgataa agtcggcaaa ctgggggttg caccgttaac cagtatgttc
     4981 ctgtttgggc cgaaccaacc gtcgcctgca aataactatc gtccggagtt gcacgactct
     5041 aacggtctgt ctatccatgc tggtaatggc gaatggatct ggcgtccgtt gaataacccg
     5101 aaacatttag cggtcagcag cttctccatg gaaaacccgc gaggctttgg tctgttgcaa
     5161 cgaggtcgtg atttctcccg ctttgaagat ctcgatgatc gttacgatct ccgtccaagc
     5221 gcatgggtga ctccgaaagg ggagtggggc aaaggcagcg ttgagctggt ggaaattcca
     5281 accaacgatg aaaccaacga taacatcgtc gcttactgga cgccggatca gctgccggag
     5341 ccgggtaaag agatgaactt taaatacacc atcaccttca gccgtgatga agacaaactg
     5401 catgcgccag ataacgcatg ggtgcaacaa acgcgtcgtt caacggggga tgtgaagcag
     5461 tcgaacctga ttcgccagcc tgacggtact atcgcctttg tggtcgattt taccggcgca
     5521 gagatgaaaa aactgccaga ggataccccg gtcacagcgc aaaccagcat tggtgataat
     5581 ggtgagatag ttgaaagcac ggtgcgctat aacccggtta ccaaaggctg gcgtctggtg
     5641 atgcgtgtga aagtgaaaga tgccaagaaa atcactgaaa tgcgtgctgc gctggtgaat
     5701 gccgatcaga cgttgagtga aacctggagc taccagttac ctgccaatga ataagacaac
     5761 tgagtacatt gacgcaatgc ccatcgccgc aagcgagaaa gcagcattgc cgaagactga
     5821 tatccgcgcc gttcatcagg cgctggatgc cgaacaccgc acctgggcgc gggaggatga
     5881 ttccccgcaa ggctcggtaa aggcgcgtct ggaacaagcc tggccagatt cacttgctga
     5941 tggacagtta attaaagacg acgaagggcg cgatcagctt aaggcgatgc cagaagcaaa
     6001 acgctcctcg atgtttcccg acccgtggcg taccaacccg gtaggccgtt tctgggatcg
     6061 cctgcgtgga cgcgatgtca cgccgcgcta tctggctcgt ttgaccaaag aagagcagga
     6121 gagcgagcaa aagtggcgta ccgttggtac catccgccgt tacattctgt tgatcctgac
     6181 gctcgcgcaa actgttgtcg cgacctggta tatgaagacc attcttcctt atcagggttg
     6241 ggcgctgatt aatcctatgg atatggttgg tcaggatttg tgggtttcct ttatgcagct
     6301 tctgccttat atgctgcaaa ccggtatcct gatccttttt gcggtactgt tctgttgggt
     6361 gtccgccgga ttctggacgg cgttgatggg cttcctgcaa ctgcttattg gtcgcgataa
     6421 atacagtata tctgcgtcaa cagttggcga tgaaccatta aacccggagc atcgcacggc
     6481 gttgatcatg cctatctgta acgaagacgt gaaccgtgtt tttgctggct tgcgtgcaac
     6541 gtgggaatca gtaaaagcca ccgggaatgc caaacacttt gatgtctaca ttctgagtga
     6601 cagttataac ccggatatct gcgtcgcaga gcaaaaagcc tggatggagc ttatcgctga
     6661 agtcggtggc gaaggtcaga ttttctatcg ccgccgccgt cgccgcgtga agcgtaaaag
     6721 cggtaatatc gatgacttct gccgtcgctg gggcagccag tacagctaca tggtggtgct
     6781 ggatgctgac tcggtaatga ccggtgattg tttatgcggc ctggtgcgct tgatggaagc
     6841 caacccgaac gccgggatca ttcagtcgtc gccgaaagcg tccggtatgg atacgctgta
     6901 tgcgcgctgt cagcagttcg cgacccgcgt gtatgggcca ctgtttacag ccggtttgca
     6961 cttctggcaa cttggcgagt cgcactactg gggacataac gcgattatcc gcgtgaaacc
     7021 atttatcgag cactgcgcac tggctccgct gccgggcgaa ggttcctttg ccggttcaat
     7081 cctgtcacat gacttcgtag aagcggcgtt gatgcgccgt gcaggttggg gggtctggat
     7141 tgcttacgat ctcccgggtt cttatgaaga attgccgcct aacttgcttg atgagctaaa
     7201 acgtgaccgc cgctggtgcc acggtaacct gatgaacttc cgtctgttcc tggttaaggg
     7261 tatgcacccg gttcaccgtg cggtgttcct gacgggcgtg atgtcttatc tctccgctcc
     7321 gctgtggttt atgttcctcg cgctctctac tgcattgcag gtagtgcatg cgttgaccga
     7381 accgcaatac ttcctgcaac cacggcagtt gttcccggtg tggccgcagt ggcgtcctga
     7441 gctggcgatt gcactttttg cttcgaccat ggtgctgttg ttcctgccga agttattgag
     7501 cattttgctt atctggtgca aaggaacgaa agaatacggc ggcttctggc gcgttacatt
     7561 atcgttgctg ctggaagtgc tgttttccgt gctgctggct ccggtacgca tgctgttcca
     7621 tacggtcttc gttgtcagcg cgttccttgg ctgggaagtg gtgtggaatt caccgcagcg
     7681 tgatgatgac tccacttcat ggggcgaagc gttcaaacgc cacggctcac agctgctgtt
     7741 ggggttagtg tgggctgttg ggatggcgtg gctggatctg cgtttcctgt tctggctggc
     7801 accgattgtc ttctcgttga tcctgtcacc gtttgtttcg gtgatttcca gccgtgccac
     7861 cgttggtctg cgcaccaaac gctggaaact gttcctgatc ccggaagagt attcaccgcc
     7921 gcaggtgctg gttgataccg atcggttcct tgagatgaat cgccaacgct cccttgacga
     7981 tggctttatg cacgcagtgt ttaacccgtc atttaacgct ctggcaaccg caatggcgac
     8041 agcgcgtcac cgcgccagta aggtgctgga aatcgcccgt gaccgccacg ttgaacaggc
     8101 gcttaacgag acgccagaga agctgaatcg cgatcgtcgc ctggtgctgc taagcgatcc
     8161 ggtgacgatg gcccgtctgc atttccgcgt ctggaattcc cctgaaagat attcttcatg
     8221 ggtgagttat tacgaaggga taaagctcaa tccactggca ttgcgtaaac cggatgcggc
     8281 ttcgcaataa aaacgtagtt gcctgatgcg ctacgcttat caggcctaca tcgttcctgc
     8341 aatttattga ttttgcaaga ttttgtaggt cggataaggc gttcacgcca catccggcaa
     8401 aaacaacgag ccaataaaaa taccggcgtt atgccgtaat gccagtcagt taagcaactg
     8461 actggctctt tttcggggct gtggggtatt tccagggcct ctcctttacc actctcgatc
     8521 gataggaatt aaaaccccaa aaagattaaa aaaaca
//
LOCUS       DAJTQQ010000145         8364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000145 DAJTQQ010000000
VERSION     DAJTQQ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.145"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            1..957
                     /gene="espL1"
                     /locus_tag="OO309_003722"
                     /pseudo
     CDS             1..957
                     /gene="espL1"
                     /locus_tag="OO309_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1129..1236
                     /locus_tag="OO309_003723"
     CDS             1129..1236
                     /locus_tag="OO309_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848708.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1289..2047)
                     /locus_tag="OO309_003724"
     CDS             complement(1289..2047)
                     /locus_tag="OO309_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR5848709.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2334..3263
                     /gene="pfkB"
                     /locus_tag="OO309_003725"
     CDS             2334..3263
                     /gene="pfkB"
                     /locus_tag="OO309_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR5848710.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3364..3654
                     /gene="ghoS"
                     /locus_tag="OO309_003726"
     CDS             3364..3654
                     /gene="ghoS"
                     /locus_tag="OO309_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR5848711.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3760..4620
                     /locus_tag="OO309_003727"
     CDS             3760..4620
                     /locus_tag="OO309_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR5848712.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4661..5197)
                     /locus_tag="OO309_003728"
     CDS             complement(4661..5197)
                     /locus_tag="OO309_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR5848713.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5344..6012
                     /gene="hxpB"
                     /locus_tag="OO309_003729"
     CDS             5344..6012
                     /gene="hxpB"
                     /locus_tag="OO309_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR5848714.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6175..6765
                     /locus_tag="OO309_003730"
     CDS             6175..6765
                     /locus_tag="OO309_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5848715.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6898..8288
                     /gene="tcyP"
                     /locus_tag="OO309_003731"
                     /pseudo
     CDS             6898..8288
                     /gene="tcyP"
                     /locus_tag="OO309_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
BASE COUNT         2169 a         2001 c         2007 g         2187 t
ORIGIN      
        1 atgctcagac ctgggctgat aggtatgttc gcaactgtgc aattgctatt ctgcccctat
       61 tacctgtggg agttctaggg gctattttcg cgggtattgc atcacgaatg ccatttgccc
      121 ctatacatcc atcggcaatg ttgttaataa tggctacagg ccagactcaa cagcttatta
      181 cattattcaa acagttgccc atactccctg aaaaagaaat cattgaaata ataactgcgc
      241 agaatagcgt tggtacacct gctttatttt tggctatgat gaacggacat actgacaacg
      301 tgaaaatatt tatgcaagaa attcagtcac tggtagataa tcacatcatt catgaagata
      361 atctggttaa attactgcaa actaaaagtg ctaacgaaac acctggactt tatatctcca
      421 tgttgtatgg attcgatgaa ataatcgata tctttctgaa tacattagcc actccaatag
      481 cactaagagc ttttaaacaa aaaactggtg atgagtattt tagccatgaa aatacatgat
      541 ggtgagccag gattatacgc cgcaatggaa aataatcacc ctttgtgtgt cacacggttc
      601 ctctctaaaa ttaatggcat cgcctttaaa tacaagttga gcaaagctaa catcatggat
      661 ttattaaaag gagctacagc acagggaacc cctgcattat acatcgccat gagcaagggt
      721 aatgaagacg tcgtgttatc ttatatatcg acgctgggtg cttttgcaaa aaaacattct
      781 tttagtcaac atcagttatt tacactattg gccgctaaaa atcatgacaa catgtcagct
      841 gttcatatag ccattcatca taagcattat aaaactgtag aaacatatta tgctgctata
      901 aatgtcatca gccaaagcct gagttttagt gctgatgaaa taaagacgta tttataacca
      961 gctatttacg atatatatcg atacataatt ttattttttt cataaaacgc agaaacagtg
     1021 accatgtgca caacgccacc cgacggctgt aataacaaaa aatcccttga tgcctgtccc
     1081 ttttgttaca ctccgttatc acgcacaaga gatatgcagg acactggtat gccgactaaa
     1141 cgctttgata aaaaacactg gaagatggtg gtggtgctac tggcaatctg tggcgctatg
     1201 ttgttgctac gttgggcagc aatgatttgg ggctgagcag tgacaaacga agacagccag
     1261 accgcataac acggtccggc agataatttt acatgctgta gcccagcgat aaggttgtcc
     1321 gacggtcggt atgttccggt gcagactctg gaggttcaga gttccatgtg acgttgtaag
     1381 ccaccttcag tccaaaatgt tcattaatag caacatttaa tgcgctttcg gagttcagcg
     1441 ttgtgtcttc cgcgccaaag accgaaacac cctgcgtaaa tttagcgttg tcagtcaact
     1501 gccatgcata agccccggag gcatagccca gcggctgggt ttcactggca ttgtcggtat
     1561 atttgtcgta acgcacacct ggaccgaatt caaagcggaa actgtgtacc gggccattca
     1621 aaaactgacg accataaccc gcggtcaaca catcgcgctc tcgatagccg ttataacggt
     1681 ctgtcagcca gcttgcctga ccaaataaat agtcataatc agttaaatta aagcggctac
     1741 gcccgcccgc cgcatatttt tctgaagaac gctcatcatt agaagaagta ttactggcgt
     1801 tcccccacag cgaccaggca gtggtttgtc cataccaggt catggtggtg tcagccgtaa
     1861 gtgaggagct tttcgtattg cctgattgtg caagatatcc tgcgttcaga ttaccttcga
     1921 aaggtttttt agcgctggca ggatcgtcca tgacagtaaa aacggaatca tcggcagctg
     1981 cattcagtga cgcaaacatg ccccccgcca acataacgat ggcaggaact gtcttcaaaa
     2041 gcttcattta tcaagagtcc gtacaacaaa aaaagagacc atcgcggtcc cggaaacttt
     2101 cttaaggatc aaagattagc gtccctggaa aggtaacgaa ttataaaaag gcgcgaataa
     2161 cttagcaatg tattcttatt tcattttttg aataagcatg tggcgaaaac agatttttat
     2221 ttatatatat ttatctgcaa aattttaaat aaagctctaa taaatcatat tgttaatttc
     2281 ttcactttcc gctgattcgg tgccagactg aaatcagcct ataggaggaa atgatggtac
     2341 gtatctatac gttgacactt gcgccctctc tcgatagcgc aacaattacc ccgcaaattt
     2401 atcccgaagg aaaactgcgc tgtaccgcac cggtgttcga acccgggggc ggcggcatca
     2461 acgtcgcccg cgccattgcc catcttggag gcagtgccac agcgatcttc ccggcgggtg
     2521 gcgcgaccgg cgaacacctg gtttcactgt tggcggatga aaatgtcccc gtcgctactg
     2581 tagaagccaa agactggacc cggcagaatt tacacgtaca tgtggaagca agcggtgaac
     2641 agtatcgttt tgttatgcca ggcgcggcat taaatgaaga tgagtttcgc cagcttgaag
     2701 agcaagttct ggaaattgaa tccggggcca tcctggtcat aagcggaagc ctgccgccag
     2761 gtgtgaagct ggaaaaatta acccaactga tttccgctgc gcaaaaccaa gggatccgct
     2821 gcatcatcga cagttctggc gaagcgttaa gtgcagcact ggcgattggt aacatcgagt
     2881 tggttaagcc taaccaaaaa gaactcagtg cgctggtgaa tcgcgaactc acccagccag
     2941 acgatgtccg caaagccgcg caggaaatcg ttaatagcgg caaggccaaa cgggttgtcg
     3001 tttcccttgg tccacaagga gcgctgggtg tcgatagtga aaactgcatt caggtggtgc
     3061 caccaccggt gaaaagccag agtaccgttg gcgctggtga cagcatggtc ggcgcgatga
     3121 cactgaaact ggcagaaaat gcctctcttg aagagatggt ccgttttggc gtagctgcgg
     3181 ggagtgcagc cacactcaat cagggaacac gtctgtgctc ccatgacgat acgcaaaaaa
     3241 tttacgctta cctttcccgc taacaaaaac attcccccag cattggggga atcaccacca
     3301 acctgtcggc aacgcgtttc tccgactatg ctcaaaagtc atgtgataac aaaggggtga
     3361 actatggcca gtggcgatct tgtccattat gtcataaccg tcatgttgca tgaggataca
     3421 ttgactgaaa ttaacgagtt gaataattac ctgactcgcg acggtttttt gctcaccatg
     3481 acggatgatg agggaaatat ccatgagctg gggactaaca cttttggact tatcagtacc
     3541 caaagtgaag aagaaattag agaactggtt tcggggctta cccaaagtgc aaccggcaaa
     3601 gatcctgaaa tcaccatcac gacctgggag gaatggaata gcaacagaaa ataaatggtt
     3661 tttgggcaat aatcagtctg tggtgtgcgt tagctcgtgt ttttacaccg cattcttgcg
     3721 ctaaccttat gatctggcag acaacatggg agagacatca tgtggcaggc aatcagtcgt
     3781 cttttgagcg agcagttagg tgaaggcgaa atcgaactgc gtaatgaact gcctggcgga
     3841 gaagtccatg ccgcatggca tttgcgctat gcaggacatg actttttcgt caaatgtgat
     3901 gaaagggaac tgcttcccgg ttttaccgcc gaagccgacc aactggagtt actgtcgcgt
     3961 agtaaaaccg tcaccgtgcc taaggtttgg gcagtaggcg ctgaccgtga ctacagtttt
     4021 ctggtgatgg attatctccc acctcgtccg ctggatgcgc atagcgcatt tattcttggt
     4081 cagcaaattg cgcgtttaca tcaatggagt gaccaaccac aatttggcct cgattttgat
     4141 aactcgctct ccacaactcc acagcccaac acctggcaac gtcgctggtc aacgtttttt
     4201 gctgaacaac ggattggctg gcagttggaa ctggcagcag agaaagggat cgctttcggc
     4261 aatatcgacg ccatcgtcga gcatattcag cagcgtctgg cctcgcatca accgcagcct
     4321 tctctgttgc acggcgattt atggtccggc aactgtgcgc tgggtccgga tggcccgtac
     4381 attttcgacc ctgcctgcga ctggggtgac cgagagtgcg acctagcgat gttaccgctg
     4441 catactgaac agccgccaca aatctatgac ggctatcagt cagtatcccc gctacctgcc
     4501 gatttccttg aacgtcaacc ggtttaccaa ctctacacgc tgttaaatcg tgcaagatta
     4561 tttggcggtc agcatttggt tattgctcag cagtcattgg atagattatt agcagcatga
     4621 tatgggttga ggataatggc cgctccgtgc ggccttttga ttaaataaac ccaagcagag
     4681 aaaagaacac ataacctgca gcaatacaaa taaccggcag gatatatagt gagaaaaact
     4741 gcaggaaaat agtatggtgc ggaacaacaa tacgggactc aatttgttca cgcgttagcc
     4801 cctcttcccc tttggctttt tccagaatga gttgatcttc aacaccttcc cgcaagaagc
     4861 gcgcctggcg actcatccgg gcaccggaat cctgcaacgc cagtccgaca aaaatcagga
     4921 tgaaaatcac ccagaacata acgttcacac caccattaaa attaggggtc ggggagttat
     4981 accagaacag attcaaaaaa ggcgtattcg cctgcatcat atcgatcatg acatgagcaa
     5041 agtcgagcat gaccgcatta atgccttcct ggttttcctg ccgggtattc ataaatttca
     5101 gcaaagaaat caatgtagag atcaatgcag gtataaaaat cacccaaccc aaaatccttt
     5161 tcaaaacagc aatgcgtcca gcttgttgat acgtcatgag ttctccttga ttaagacgcg
     5221 tcgtttcact tagtttacct gtagatatct gttttcgccc attctttaaa ggcgatatga
     5281 taggcgctta atcataagca cggcttaata ccttacacat aatgctctaa aggagaggtt
     5341 gtaatgtcaa ccccgcgtca gattcttgct gcaatttttg atatggatgg attacttatc
     5401 gactcagaac ctttatggga tcgagccgaa ctggatgtga tggcaagcct gggggtagat
     5461 atctcccgtc gtaacgagct gccggacacc ttaggtttac gcatcgatat ggtggtcgat
     5521 ctttggtacg cccggcaacc gtggaatggg ccaagccgtc aggaagtagt agaacgggtt
     5581 attgcccgtg ccatttcact ggttgaagag acacgtccat tattaccagg cgtgcgcgaa
     5641 gctgttgcgt tatgcaaaga acaaggttta ttggtgggac tggcctccgc gtcaccacta
     5701 catatgctgg aaaaagtgtt gaccatgttt gacttacgcg acagtttcga tgccctcgcc
     5761 tcggctgaaa aactgcctta cagcaagccg catccgcaag tatatctcga ctgcgcagca
     5821 aaactgggcg ttgacccact gacctgcgtg gcgctggaag actcggtaaa tggcatgatc
     5881 gcttctaaag cagcccgcat gcgttccatc gtcgttcctg cgccagaagc gcaaaatgat
     5941 ccacgttttg tattagcaaa cgtcaaactc tcatcgctga cagaactcac cgcaaaagac
     6001 cttctcggtt aatgaccagg ggcagtgatc acgctgcccc tggttcttta tctgaatttg
     6061 cattcaactg acggattaat cgtcaattta agagaaagag ttacaccgtc accacttccg
     6121 tgcactgtat aaaaatccta tactgtacgt atcgacagtt tagtgagttt tatcatgacg
     6181 gcggaaggtc accttctctt ttctattgct tgtgcggtat ttgccaaaaa tgccgagctg
     6241 acgcccgtgc tggcacaggg tgactggtgg catattgtcc cttccgcaat cctgacgtgt
     6301 ttgttaccgg acatcgatca cccaaagtcg tttcttgggc agcgattaaa atggatatca
     6361 aaaccgatcg cccgcgcttt tgggcatcgt ggttttaccc acagtctgct ggcggtattt
     6421 gcgctgctgg caacctttta ccttaaggtt ccggaaggct ggttcattcc ggctgatgcg
     6481 ctacaaggaa tggtgctggg ttatttgagc cacatacttg ccgatatgct gacacccgcc
     6541 ggtgttcccc tgctctggcc atgccgctgg cgtttccgct tgcctatcct ggttccccaa
     6601 aagggcaacc aactggaacg ttttatctgc atggcattat ttgtctggtc ggtatggatg
     6661 ccccattcat tacccgagaa cagcgctgtt cgttggtcat cgcaaatgat caataccttg
     6721 cagatccagt ttcatcggct tattaagcat caggttgaat actaaaaagg caaaaatcac
     6781 ctttctggaa taagcaattc catttgaata taagagccag ctcacagttc tgttaatctt
     6841 gcgccaacac tatgactgct acgcagtgat agaaataata agatcaggag aacggggatg
     6901 aactttccat taattgcgaa catcgtggtg ttcgttgtac tgctgtttgc gctggctcag
     6961 acccgccata aacagtggag tctggcgaaa aaagtgctgg tgggtctggt gatgggtgtg
     7021 gtttttggcc ttgccctgca taccatttat ggttctgaca gccaggtact taaagattct
     7081 gtacagtggt ttaacatcgt tggtaacggc tatgttcaac tgctgcaaat gatcgttatg
     7141 ccgttagtct tcgcctctat tctgagcgcg gttgcccgtc tgcataacgc atctcagtta
     7201 ggcaaaatca gttttctgac catcggtacg cttttgttta ccacgctgat tgcggcgctg
     7261 gtcggtgtgc tggtcaccaa cctgtttggt ttgacggctg aaggtctggt tcagggtggt
     7321 gcagaaactg cacgtctgaa cgccattgaa agtaactatg ttggtaaagt ctctgatcag
     7381 agcgttccgc agctggtctt gtcctttatc ccgaaaaacc cgtttgccga tcttaccgga
     7441 gccaatccga cgtcaattat cagcgtggta atttttgccg cattcctcgg cgtagctgca
     7501 ttgaaactgc tgaaggatga tgcgccgaaa ggggaacgcg tcttaaccgc tatcgatacc
     7561 ctacaaagct gggtgatgaa actggttcgc cttgtcatgc agttgacccc ttacggcgtt
     7621 ctggctctaa tgaccaaagt ggttgcaggt tctaacttgc aagacatcat caaactggga
     7681 agtttcgttg tcgcgtccta cctcggtctg ctgattatgt ttgctgtgca tggcattctg
     7741 ctgggcatta atggcgtgag tccgctgaag tacttccgta aggtatggcc tgtgctgacg
     7801 tttgccttta ccagccgttc cagtgctgcg tctatcccac tgaatgtgga agcacaaacg
     7861 cgtcgtctgg gcgttcctga atccatcgcc agtttcgccg cctctttcgg tgcaaccatt
     7921 ggtcagaacg gctgcgccgg tttgtatccg gcaatgctgc ggtgatggtt gcgcctacgg
     7981 ttggcattaa cccgctggac ccgatgtgga ttgcgacgct ggtcggtatt gttaccgtta
     8041 gttccgcagg cgttgccggt gtcggtggtg gtgcaacttt cgccgcactg attgtgctgc
     8101 ctgcgatggg cctgccagta accctggtgg cgctgttaat ctccgttgaa ccgcttatcg
     8161 acatgggccg tacggcgcta aacgttagtg gctcgatgac agctggcacg ctgaccagcc
     8221 agtggctgaa gcaaaccgat aaagccattc tggatagcga agacgacgcc gaactggcac
     8281 accgttaatt cttatgctgg caacggtccg ttttgtatag gggccgttgc tttacttttt
     8341 aaatgttcca tcgcggcgat gcat
//
LOCUS       DAJTQQ010000146         8333 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000146 DAJTQQ010000000
VERSION     DAJTQQ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.146"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..159
                     /locus_tag="OO309_003732"
     CDS             <1..159
                     /locus_tag="OO309_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848716.1"
                     /translation="ISESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGE
                     DVEPGDDF"
     gene            complement(168..566)
                     /gene="vapC"
                     /locus_tag="OO309_003733"
     CDS             complement(168..566)
                     /gene="vapC"
                     /locus_tag="OO309_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR5848717.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(566..793)
                     /gene="vapB"
                     /locus_tag="OO309_003734"
     CDS             complement(566..793)
                     /gene="vapB"
                     /locus_tag="OO309_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR5848718.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            875..1246
                     /locus_tag="OO309_003735"
     CDS             875..1246
                     /locus_tag="OO309_003735"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR5848719.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEASAST"
     gene            1267..6128
                     /gene="traI"
                     /locus_tag="OO309_003736"
                     /pseudo
     CDS             1267..6128
                     /gene="traI"
                     /locus_tag="OO309_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6148..6894
                     /gene="traX"
                     /locus_tag="OO309_003737"
     CDS             6148..6894
                     /gene="traX"
                     /locus_tag="OO309_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR5848720.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6949..7509
                     /locus_tag="OO309_003738"
     CDS             6949..7509
                     /locus_tag="OO309_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR5848721.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7640..7852
                     /locus_tag="OO309_003739"
     CDS             7640..7852
                     /locus_tag="OO309_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR5848722.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8096..>8333
                     /locus_tag="OO309_003740"
     CDS             8096..>8333
                     /locus_tag="OO309_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5848723.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1913 a         2086 c         2671 g         1663 t
ORIGIN      
        1 atcagtgaat ccggtgaagt ggtggatatg gccgcttatg aggcatggca gcaggaaaat
       61 catccggaca cctggcagca gatgcagcgt cgtgaagagg tgaacatcaa tgtgcaccgg
      121 gaacgcgggg aggatgttga gccgggagat gatttctgat gaacaggtca gctccagtct
      181 tcagttctca ggccgcccac acgttcaaat tcccgggtgt tattagtcac aataatcagc
      241 ccccgactgc gggcatgacc tgcgatcatt tgatcaaatg gcccgacagg gcgtccctga
      301 cgggcaagtt ctgctcttat ctggccggtg tgtgtggctg cagcagcgtc gtaatccaga
      361 acatcaatgc gggaaacaaa tccctcgatc acagcgagat tacgttcagg catctggctt
      421 ttttctgcac catatatcag ctccatcagg gtgaccgaac tgatacacat tttcccctgg
      481 ttcaggttaa aacgctccct gacgctggcg ggtttgttct ttatcgtaaa aatgcagatg
      541 ttggtatcga gcataaactt cagcatcaga atgactccct ttcttgcatg ccgggctgtt
      601 ccctgttatc cataaaatcg gtgctgacgc tgtgtccgtc gaaccattcg tcccatgtct
      661 ctccggctgg cgtaatgatt ctggttcgtc cgacagcaat cacttcaacg cgttttacgt
      721 tttccggcaa tgcaaccgct tttggcagtc tgaccgcctg gctgcggttg ctgagaaata
      781 cggtggtttc cattatttgc ctccttatgt ggatatgtct tgtgtatatc tattgtggga
      841 ttgataccgg gatatgtcaa agggatatac gtttatgatg agtatcgcgc aggtcagatc
      901 ggccggaagt gccggtaatt attataccga caaggataat tactatgtgc tgggcagcat
      961 gggagaacgc tgggccggcc agggggctga acaactgggg ctgcagggca gtgtcgataa
     1021 ggatgtcttt acccgccttc tggagggcag gctgccggac ggggcggatt taagccgcat
     1081 gcaggatggc agtaacaggc atcgtcccgg ctacgacctg accttctccg ctcccaaaag
     1141 tatctccatg atggccatgc tcggggggga caaacgtctt attgaagcac ataaccaggc
     1201 cgtggatttt gctgtccgtc aggtggaggc cagtgcctcc acctgacgga cggacagtca
     1261 gaaacggtgc tgaccggtaa tctggtgatg gcactgttta accacgacac cagtcgcgat
     1321 caggaaccac agttacacac gcatgcggtg gtgactaatg ttacgcagta taacggcgaa
     1381 tggaagacac tgagcagtga caaagtgggg aaaacggggt tcattgagaa tgtgtacgct
     1441 aatcagattg cctttggcag gctctaccgg gaaaaactga aagagcaggt tgaggcgctg
     1501 ggctatgaaa ctgaagtggt cggtaagcac ggtatgtggg aaatgccggg tgtaccggtg
     1561 gaggcctttt ccggacgctc acagactatc cgggaggccg tcggggaaga cgcctcgctg
     1621 aaatcccggg atgtggcggc gctggatacg cgtaaatcca aacagcacgt cgatcctgaa
     1681 gtcagaatgg ccgagtggat gcagacgctg aaggaaaccg ggttcgacat ccgggcatat
     1741 cgtgacgcag cggaacaacg ggcatatacc cgcacgcaga cacccggacc cgcttcacag
     1801 gacgggccgg atgtgcagca ggcggtgaca caggccattg ccggattaag tgaacgcaaa
     1861 gtgcagttta tgtatacgga cctgctggcc aggacggtcg gcatactgcc gccggaaaat
     1921 ggtgtgattg aacgggcacg cgccggtatc gatgaggcca tcagccgtga gcagcttatc
     1981 cccctcgatc gtgagaaggg gctgttcacg ttcgggattc atatgctcga tgagctgtca
     2041 gtccgggcac tcagtcgtga catcatgaaa cagaaccggg tgaccgtaca tccggagaaa
     2101 agtgtccccc ggacggccgg ttacagcgat gccgtcagtg ttctggcaca ggaccgtccg
     2161 tcgctggcca ttgtgtccgg gcagggcggt gcagccgggc agcgtgagcg ggtggctgaa
     2221 ctggtcatga tggcccggga gcaggggcgg gaggtgcaga ttatcgctgc tgaccgtcgc
     2281 tcgcagatga acctgaagca ggatgaacgg ctgtccggtg aactgataac cggacgtcgt
     2341 cagttgctgg aaggaatggc cttcacgccg ggcagtaccg ttatcgtcga ccagggcgaa
     2401 aaactctccc tgaaagagac gttaaccctg ctggatggtg ccgcacgtca taacgtacag
     2461 gtcctgataa ccgacagcgg gcagcgaacc ggtacaggca gtgcactgat ggccatgaag
     2521 gatgccgggg tgaacacata tcgctggcag gggggagaac agcgaccggc caccatcatc
     2581 agtgaaccgg accgtaatgt cggctggccg gagattttgc ggccagcgtg aaagccggag
     2641 aagagagcgt ggcacaggtc agcggagtac gggaacaggc catactgaca caggccattc
     2701 gcagtgagct gaaaacacag ggcgtgctcg gacacccgga ggtaaccatg accgccctgt
     2761 caccggtctg gctggacagc cggagccgtt atctgcggga tatgtaccgc ccgggaatgg
     2821 tgatggagca gtggaacccg gagacacgca gtcatgaccg ctatgtgaca gaacgtgtga
     2881 cggcgcagag tcacagcctg accctgcgga atgcgcaggg cgaaacgcag gtggtgcgta
     2941 tttcttccct ggacagcagc tggtcgctgt tccggccgga aaaaatgccg gtggcagacg
     3001 gtgagcgtct gagggtgaca gggaaaattc ccggactccg cgtctccggc ggtgaccgcc
     3061 tgcaggtggc atccgtcagt gaagatgcga tgacggttgt tgtgccggga cgggctgaac
     3121 cggccaccct gcctgtgagc gattcaccgt tcactgcact gaagctggag aacggctggg
     3181 tggaaacgcc cgggcattcc gtcagcgaca gtgcgacggt ttttgcctcc gtcacacaga
     3241 tggcaatgga caatgccacc ctgaatggtc tggcccgcag tggtcgtgat gtccggctgt
     3301 attcctcact ggatgaaacc cgtactgcgg aaaaacttgc ccgccatccc tcctttacgg
     3361 tggtttctga gcagataaag gcgcgtgccg gtgagacatt gctggaaacc gctatcagtc
     3421 tgcagaaagc cgggcttcac acgccggcac agcaggccat tcatctggcc cttcctgtgc
     3481 tggaaagtaa aaacctggcc ttcagcatgg tggacctgct gacagaggcg aagtcgtttg
     3541 ctgcagaagg aaccggtttt gctgacctgg gaggggaaat caatgcgcag ataaaacggg
     3601 gtgatttact gtatgtggat gtggcaaaag gctatggcac aggcctgctg gtttcccgtg
     3661 cgtcgtatga ggcagaaaag agcattcttc gccatattct cgaaggtaag gaggcggtca
     3721 cgccgctgat ggagagagta cctggcgaac tcatggagaa actgacatca ggacagcgcg
     3781 ccgccacccg tatgatactg gaaacgtctg accgtttcac ggtggtgcag ggctatgccg
     3841 gtgtgggtaa gaccacacag ttccgggcgg tgatgtcagc cgtgaacatg ctgccggaga
     3901 gtgaacgtcc ccgtgtcgtg gggctggggc ccacacaccg tgcggtcggg gagatgcgca
     3961 gcgccggcgt ggatgcacag acactggcgt cctttctgca tgacacgcag ctgcagcagc
     4021 gcagcggaga aactccggat ttcagcaaca cgctgttcct tctcgatgag agctctatgg
     4081 tgggcaatac cgacatggca cgggcatacg ccctgattgc ggccggtggc ggtcgggctg
     4141 tggccagcgg tgacacggac cagctgcagg ccatcgcgcc cggtcagcct ttccgtctcc
     4201 agcagacgcg cagtgctgcc gatgtggtca tcatgaagga gattgtgcgt cagacgccgg
     4261 aactgcggga ggcggtatac agcctgatta accgggatgt ggaaagggcg ctgtccgggc
     4321 ttgagcgtgt gaaaccgtct caggtgccac gtctggaggg cgcatgggca ccggagcact
     4381 ccgtgacgga gttcagtcac agccaggaag cgaaactggc agaagcgcag cagaaggcga
     4441 tgctgaaagg cgaggctttt ccggatgtcc ccatgacact gtatgaagcc attgtccgcg
     4501 actataccgg caggacaccg gaagcacggg agcagacgct gattgtcacg cacctgaatg
     4561 aggaccggcg cgtactgaac agcatgattc atgatgcacg ggaaaaggcc ggagaactgg
     4621 ggaaggtgca ggtcatggtg cctgtcctga acacagcgaa tatacgcgac ggggagctgc
     4681 gtcgtctctc cacctgggag aataacccgg atgcccttgc cctggtggat aatgtgtatc
     4741 accggattgc cggtatcagt aaggatgacg ggctgataac cctgcaggat gcggaaggta
     4801 acacgcggct gatttcgccc cgggaggcgg tggctgaagg cgtcacgctg tacaccccgg
     4861 acaccatccg ggtggggacc ggtgacagga tacgcttcac gaagagtgac cgggagcgcg
     4921 gttatgtggc caacagcgtc tggacggtga cagcggtttc cggtgacagt gtcacacttt
     4981 ctgacggtca gcagacccgt gtgattcgcc ccggtcagga gcgggcagag caacatattg
     5041 acctggccta tgccatcacc gcccacggtg cacagggggc gagtgaaacc tttgccatcg
     5101 cgcttgaagg cacggaaggt aaccggaaac tgatggccgg ctttgagtca gcctacgtgg
     5161 ccctgtcgcg tatgaagcag catgtgcagg tgtacaccga taaccgtcag ggctggacgg
     5221 atgccatcaa caatgccgta cagaaaggaa ctgcccacga tgtgtttgag ccgaaaccgg
     5281 accgggaggt catgaatgca gagcggctgt tcagtacggc gcgggagctg cgggacgtgg
     5341 cggcaggccg tgccgttctc cgtcaggcgg ggctggccgg gggagacagt cctgcacggt
     5401 ttattgctcc gggacgtaaa tatccgcaac cgtatgtggc actgccggcg tttgaccgta
     5461 acggcaagtc cgccggtatc tggctgaacc cgctgaccac ggatgacgga aacgggctgc
     5521 ggggattcag cggtgaaggt cgggtaaaag gcagcgggga tgcgcagttc gtggccctgc
     5581 agggcagccg taacggagaa agcctgctgg ctgataatat gcaggatggc gtccggattg
     5641 cccgtgataa tcctgacagt ggtgtggtgg taagaatcgc cggtgaaggc cgtccgtgga
     5701 atcccggtgc catcaccggt ggccgcgtgt ggggggatat cccggacaac agcgtccagc
     5761 cgggagccgg aaatggtgaa ccggtcacgg cagaggtact ggcacagcgg caggctgaag
     5821 aggccatccg ccgtgaaacg gaacgtcgcg cagatgaaat tgtccgtaaa atggcagaga
     5881 acaaacctga cctgccggac ggcaaaacag agcaggctgt cagggaaatt gccgggcagg
     5941 agcgtgaccg ggctgccata actgaacggg aagccgcgct gccggagagt gtactgcgtg
     6001 aaccacaacg ggtgcgggaa gcggtccggg aagttgcccg ggaaaatttg ctgcaggagc
     6061 gtctgcagca gatggagcgg gatatggttc gtgacctgca gaaagagaaa accccgggcg
     6121 gagactgata caggaagaca aacactgatg acaacggata acacgaacac gacacgtaac
     6181 gattcactgg ctgcacggac cgatacctgg ttgcagtcat ttctggtctg gtcacccgga
     6241 cagcgggata tcatcaaaac ggtggcactg gtgctgatgg tactggacca catcaatctg
     6301 atattccagc tgaagcagga atggatgttt ctggccgggc gaggagcctt tccgcttttt
     6361 gccctggtgt gggggctgaa tctgtcccgt catgcgcata tccggcaacc ggccattaac
     6421 cggctgtggg gatggggaat tattgcccag ttcgcatatt atctggcagg ctttccttgg
     6481 tatgagggga atatcctgtt tgcctttgca gtggcggcac aggtgctgac gtggtgtgaa
     6541 acgcgcagtg ggtggcgtac agcagcggcc atactcctga tggccctgtg ggggcctttg
     6601 tccggcacca gttacggtat tgccgggttg ctgatgctgg cagtcagcaa ccggctgtac
     6661 cgggcggaag acagagcgga acgtctggcg ctggtcgcct gcctgctggc tgttattccg
     6721 gcgcttaacc ttgccaccag tgatgcagcg gcggtggccg gtcttgtgat gacggtgctg
     6781 accgttggtc tggtgttgtg tgccggaaaa tcattacccc gtttctggcc cggagacttt
     6841 ttcccgacgt tctatgcctg tcatctggct gtgctgggcg ttctggcgct gtgacgggtg
     6901 tggtatcttt ggccgcaaga ggatgattcg tcagaggcag aacacagcat gacagagcag
     6961 aagcgaccgg tactgacact gaagcggaag acagagggaa cagcgcctgt ccgcagccgg
     7021 aaaaccatca tcaatgtcac cacgccacca aagtggaagg tgaaaaagca gaagctggcc
     7081 gagaaagcgg cccgtgaagc agagctggcg gcaaaaaaag cgcaggccag acaggcgctg
     7141 tccatttatc tgaacctgcc cacgctggat gaggctgtga ataccctgaa gccctggtgg
     7201 ccgggattat ttgacggtga cacgccccgg cttctggcct gcggtatccg ggacgtgtta
     7261 ctggaagacg tggcgcagcg gaatatcccg ctctcgcata aaaaactgcg cagggcgctg
     7321 aaggccatca cccgttcaga aagctatctg tgtgccatga aagccggtgc ctgccggtat
     7381 gacacggaag ggtatgtgac ggagcatatt tctcaggagg aggaagcgta tgcgggagcg
     7441 cgtctggcga aaattcgtcg ccagaaccgg ataaaggcag aacttcaggc cgtgcttgat
     7501 gagaaataaa aaaagcctcc cccagagaga ggaggcaggg aaataaggct taaaaggaaa
     7561 taacttccgc ttccgaaagg aagccagtcc actgaattat ttatcacaat aatactgaca
     7621 actaaagggg gacaagaaaa tgaatggatt cagaaacagt tcacggaacg gccaggtctg
     7681 gcgttaccag cgtgccggcg ggcgggccgt tattctggaa gtcagtggac gctggatgga
     7741 agcggcagaa gcatggcgac gggctgcctg tgttgcgcct aggacagact ggcagcagtt
     7801 tgcccggaaa agggctgaac actgccaccg gcgctgccgg ggaagggtgt aacgaaaaaa
     7861 gcagccttca ttactctagg agtcagaagg ctgaaaggag tttatgagat ttgcttctta
     7921 atatccggtc gggcagtatg accggacagt tctgagtagc acagagtctt ttccgcatta
     7981 agtcgatttt ttatattttg tgattagttt cacaaaataa ggtgttattc aatgtgtgct
     8041 gcactattca ggggggctga aatacaggtg gcatttatct gatatggaga ataacatgag
     8101 gaaatatatt ccactggcat tatttatctt ttcatggccg gtattgtctg cagatattca
     8161 tggacgggtt gttcgtgtac tggacggtga taccatcgag gtcatggact cactgaaggc
     8221 ggtgcggatc aggctggtaa atattgatgc accggaaaag aaacaggatt atggccggtg
     8281 gtcaacagac atgatgaaat cactggtcgc aggaaaaacg gttaccgtca cat
//
LOCUS       DAJTQQ010000147         8248 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000147 DAJTQQ010000000
VERSION     DAJTQQ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.147"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            174..1277
                     /gene="proB"
                     /locus_tag="OO309_003741"
     CDS             174..1277
                     /gene="proB"
                     /locus_tag="OO309_003741"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR5848724.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1289..2542
                     /gene="proA"
                     /locus_tag="OO309_003742"
     CDS             1289..2542
                     /gene="proA"
                     /locus_tag="OO309_003742"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR5848725.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2657..2732
                     /locus_tag="OO309_003743"
     tRNA            2657..2732
                     /locus_tag="OO309_003743"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2690..2692,aa:Thr,seq:cgt)
     gene            complement(2747..3907)
                     /locus_tag="OO309_003744"
     CDS             complement(2747..3907)
                     /locus_tag="OO309_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5848726.1"
                     /translation="MSIKLRGGTWHCDFVAPDGSRVRRSLETSDKRQAQELHDRLKAE
                     AWRVKNLGESPKKLFKEACIRWLREKSDKKSIDDDKSIISFWMLHFREAILSDITTEK
                     IMEAVDGMENRRHRLNWEMSRDRCLRLGNPVPEYKPKLASKGTKTRHLAILRAILNMA
                     VEWGWLDRAPKISTPRVKNGRIRWLTEEESKRLFAEIAPHFFPVVMFAITTGLRRSNV
                     TDLEWSQVDLDKKMAWMHPDETKAGNAIGVPLNETACQILRKQQGLHKRWVFVHTKPA
                     YRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRFHDLRHTWASWLVQSGVSLLALK
                     EMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNGTNTAQEENVVYLNAR"
     gene            complement(3799..4131)
                     /locus_tag="OO309_003745"
                     /pseudo
     CDS             complement(3799..4131)
                     /locus_tag="OO309_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019076104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(4218..4562)
                     /locus_tag="OO309_003746"
     CDS             complement(4218..4562)
                     /locus_tag="OO309_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848727.1"
                     /translation="MSDLAMKVLKWQSTDDVGISSATLASIACGLKKNIYGHSFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            complement(4663..4842)
                     /locus_tag="OO309_003747"
     CDS             complement(4663..4842)
                     /locus_tag="OO309_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eag protein"
                     /protein_id="HCR5848728.1"
                     /translation="MSCPKCGSGNIAKEKTMRGWSGDYVCCDCGYNDSKDAFGERGKN
                     EFVKINKEREGNEKS"
     gene            complement(4939..5868)
                     /locus_tag="OO309_003748"
     CDS             complement(4939..5868)
                     /locus_tag="OO309_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR5848729.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVAY
                     IIQDSDARSRGEKGILRYFANISDEDINEYEITVTPLYAAPPETVSVPDAMEMDDDFD
                     SAFEHGKAVGWNAYRAAMLQSGNFRENKNSSTNNFREIAETSTNYPVIPSEVLSAIQK
                     VAKIRADFDDFDGDRRGIGDCLDEAEQELIVTINKYASQLAAEPIAPNDVREQTAIPQ
                     VPVTPDGWISCSERMPVIGELNWRTSFPLLITCEIGVIPAYYGFVSVNGNKHYGFMES
                     LKYGDDNGNHPQTNEYGLISNVTHWMPLPEPPL"
     gene            complement(5871..6062)
                     /locus_tag="OO309_003749"
     CDS             complement(5871..6062)
                     /locus_tag="OO309_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848730.1"
                     /translation="MDGQISIVRPGACDDREIRLIIRLAMGKTITALITPENLALALT
                     GKSDLPVELKLRNIEIKVK"
     gene            complement(6064..6693)
                     /locus_tag="OO309_003750"
     CDS             complement(6064..6693)
                     /locus_tag="OO309_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR5848731.1"
                     /translation="MSEINYQALREAAERAIPAMERLLMLPADDLLSEQELKDYGVDI
                     DALNAFKFLAGPETVLALLDERVRNQQYIKSRDQENEDIALTVGKLLIENGRLVADTL
                     RHLADNEIDSDYFAITSTNENGTEIDHEMAITDYALQAAGTVDELVAALESAENRIAE
                     LGERGILLPERSSMLHRTDFHDDYQTVMAYKVSEVIAAIRAAGIRIKGE"
     gene            complement(6690..7265)
                     /locus_tag="OO309_003751"
     CDS             complement(6690..7265)
                     /locus_tag="OO309_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001701210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hNH endonuclease"
                     /protein_id="HCR5848732.1"
                     /translation="MKQMSLIEMDGFLKGKCIPCDLKVNETNAEYLVRKFAEAEAKIS
                     ALYEDHQKAIESIKQADAAVKLAHEKFSALAAENAGMKSVIEYCINPDNQPEYHDQGM
                     GCGVEDHGYQRDGYSACYYGWESAMDRVYSEVIPDAIPETPATDTFLDEVRAQGVEMF
                     AASMKVVGGHEHPYSAMANEFAAQLRKGGGQ"
     gene            complement(7262..7426)
                     /locus_tag="OO309_003752"
     CDS             complement(7262..7426)
                     /locus_tag="OO309_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HCR5848733.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPMPSREELLKRKSFGSVNDNKY
                     LNAMWRSGKK"
     gene            complement(7437..7730)
                     /locus_tag="OO309_003753"
     CDS             complement(7437..7730)
                     /locus_tag="OO309_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HCR5848734.1"
                     /translation="MPAPLYGADDPRRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEELERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            complement(7754..8137)
                     /locus_tag="OO309_003754"
     CDS             complement(7754..8137)
                     /locus_tag="OO309_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848735.1"
                     /translation="MAHSITVRLNKPAREFQAGENIGFNIRAGVQYYDRQTKKKEWTN
                     YSAVVFAKPGAQADYYRSVLVEGGIVEITGENIRVDVYQGQNGQSITLELLNAKIGFA
                     TSGNSQQQQSSNHQNHPEYDDSIPF"
     gene            complement(8137..>8248)
                     /locus_tag="OO309_003755"
     CDS             complement(8137..>8248)
                     /locus_tag="OO309_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCR5848736.1"
                     /translation="KQAFAKAWKMLEGTPEQHKAQDIYNIRRDELEGAAA"
BASE COUNT         1897 a         2102 c         1974 g         2275 t
ORIGIN      
        1 aaaatgtggt aatttattaa atctgtaata aaagcgtaaa caactgccgc tacgcttgct
       61 gatcccgcgc aacaaaacgc catgctttgc tcgcagatgg ttggcaaccg acgacagtcc
      121 tgctaaaacg ttcgtttgat atcatttttc ctaaaattga atggcagaga atcatgagtg
      181 acagccagac gctggtggta aaactcggca ccagtgtgct aacaggcgga tcgcgccgtc
      241 tgaaccgtgc ccatatcgtt gaacttgttc gccagtgcgc gcagttacat gccgccgggc
      301 atcggattgt tattgtgacg tcgggcgcga tcgccgccgg acgtgagcac ctgggttacc
      361 cggaactgcc agcgactatc gcctcgaaac aactgctggc ggcggtaggg cagagtcgac
      421 tgattcaact gtgggaacag ctgttttcga tttatggcat tcacgtcggg caaatgctgc
      481 tgacccgtgc tgatatggaa gaccgtgaac gcttcctgaa cgcccgcgac accctgcgtg
      541 cgttgctcga taacaatatc gttccggtaa tcaatgagaa cgatgctgtc gctacggcag
      601 agattaaggt cggcgataac gataaccttt ctgcgctggc ggcgattctg gcgggtgccg
      661 ataaactgtt gctgctgacc gatcaaaaag gtttgtatac cgctgatccg cgcagaaatc
      721 cgcaggcaga actgattaaa gatgtttacg gcattgatga cgcactgcgc gcgattgccg
      781 gtgacagcgt ttcaggcctc ggaactggcg gcatgagtac taaattgcag gccgctgacg
      841 tggcttgccg tgcgggtatc gacaccatta ttgccgcggg cagcaagccg ggcgttattg
      901 gtgatgtgat ggaaggcatt tccgtcggta cgctgttcca tgcccaggcg actccgcttg
      961 aaaaccgtaa acgctggatt ttcggtgcgc cgccggcggg tgaaatcacg gtagatgaag
     1021 gggcaactgc cgccattctg gaacgcggca gctccctgtt gccgaaaggc attaaaagcg
     1081 tgactggcaa tttctcgcgt ggtgaagtca tccgcatttg caacctcgaa ggtcgcgata
     1141 tcgcccacgg tgtcagtcgt tacaacagcg atgcattacg ccgtattgcc ggacaccact
     1201 cgcaagaaat tgatgcaata ctgggatatg aatacggccc ggttgccgtt caccgtgatg
     1261 acatgattac ccgttaagga gctggccgat gctggaacaa atgggcattg ccgcgaagca
     1321 agcctcgtat aaattagcgc aactctccag ccgcgaaaaa aatcgcgtgc tggaaaaaat
     1381 cgccgatgaa ctggaagcac aaagcgaaat catcctcaac gctaacgccc aggatgttgc
     1441 tgacgcgcga gccaatggcc ttagcgaagc gatgcttgac cgtctggcac tgacgcccgc
     1501 acggctgaaa ggcattgccg acgatgtacg tcaggtgtgc aacctcgccg atccggtggg
     1561 gcaggtaatc gatggcggcg tactggacag cggcctgcgt cttgagcgtc gtcgcgtacc
     1621 gctgggggtt attggcgtga tttatgaagc gcgcccgaac gtgacggttg atgtcgcttc
     1681 gctgtgcctg aaaaccggta atgcggtgat cctgcgtggt ggtaaagaaa cctgtcgcac
     1741 taacgcggca acggtggcgg tgattcagga cgccctgaaa tcctgtggct taccggcggg
     1801 tgccgtgcag gcgattgata atcctgaccg tgcgctggtc agtgaaatgc tgcgtatgga
     1861 taaatacatc gacatgctga tcccgcgtgg tggcgctggt ttgcataaac tgtgccgtga
     1921 acagtcgaca atcccggtga tcacaggtgg tataggcgta tgccatattt acgttgatga
     1981 aagtgcagag atcgctgaag cattaaaagt gatcgtcaac gcgaaaactc agcgtccgag
     2041 cacatgtaat acggttgaaa cgttgctggt gaataaaaac atcgccgata gcttcctgcc
     2101 cgcattaagc aaacaaatgg cggaaagcgg cgtgacatta cacgcagatg cagctgcgct
     2161 gacacagttg caggcaggcc ctgcgaaggt ggtggcggtt aaagccgaag agtatgacga
     2221 tgagtttctg tcattagatt tgaacgtcaa aatcgtcagc gatcttgacg atgccatcgc
     2281 ccatattcgt gaacacggca cacaacactc cgatgcgatc ctgacccgcg atatgcgcaa
     2341 cgcccagcgt tttgttaacg aagtggattc gtccgctgtt tacgttaacg cctctacgcg
     2401 ttttaccgac ggcggccagt ttggtctggg tgcggaagtg gcggtaagca cacaaaaact
     2461 ccacgcgcgt ggcccaatgg ggctggaagc actgaccact tacaagtgga tcggcattgg
     2521 tgattacacc attcgtgcgt aaataaaacc gggtgatgca aaagtagcca tttgattcac
     2581 aaggccattg acgcatcgcc cggttagttt taaccttgtc caccgtgatt cacgttcgtg
     2641 aacatgtcct ttcagggccg atatagctca gttggtagag cagcgcattc gtaatgcgaa
     2701 ggtcgtaggt tcgactccta ttatcggcac catttaaatc aataagttac ctcgcattta
     2761 agtaaaccac gttctcctct tgtgccgtat ttgtgccatt gcgacttata atcgcatcga
     2821 ttttgcttgc gtgctcggtg agatgcccgg ctgaaaggtg ggcgtatctt tgaaccattt
     2881 cgagagtttc ccatcctccc atctctttaa gtgcaagaag agagacaccg gactgaacca
     2941 gccagcttgc ccaggtatgc ctcagatcat ggaagcggaa gttgctaatg cctgcccgct
     3001 ttaacgctcc cttccatgcc ttgttgctgt cggttctcat cttccttacc gctgctgttt
     3061 ttgttccgtc gcttcggtag gcaggtttgg tgtggacaaa tacccatctc ttatggagcc
     3121 cctgctgttt tcttaatatc tggcatgcgg tttcgttaag aggaactccg atcgcattgc
     3181 cagcttttgt ttcatcaggg tgcatccatg ccattttctt atccagatcg acctgtgacc
     3241 actcaaggtc tgtaacgttg gaacggcgaa ggcctgtcgt gattgcaaac atgaccacag
     3301 ggaagaagtg aggagcaatt tctgcaaaca ggcgcttcga ttcctcctct gtaagccatc
     3361 tgatgcgtcc attcttaacg cgtggtgttg atattttggg tgccctgtca agccatcccc
     3421 attcaacagc catattgaga atggcgcgaa gtattgccag atgcctcgtc ttcgttcctt
     3481 tgcttgcaag ctttggttta tactccggca ccggattgcc aagtcgcaaa cacctgtccc
     3541 ggctcatctc ccagttcagg cgatggcggc ggttttccat cccgtctacc gcctccatta
     3601 ttttttctgt tgttatgtca gagagaatgg cttctctgaa gtgcaacatc cagaacgata
     3661 taatgctctt gtcatcatca atggacttct tatccgattt ctcacgcagc caccgtatgc
     3721 aggcttcctt gaatagcttt ttcggtgatt ccccgagatt ttttactctc cacgcttctg
     3781 ctttcagacg atcgtgaagt tcttgcgctt gccttttgtc cgatgtttca agagagcgtc
     3841 taactcttga tccatctggc gcgacgaaat cgcagtgcca cgtgccaccg cgtagtttga
     3901 ttgacatgct ttaacctcct gcacatcaac cgcattcacc gcgctattgt gtctcacaga
     3961 cttaagcgcc gcaatgcagt ctgacttgca aatgcgatat gggcttttag gtttatctgg
     4021 atttatcttt gcggcctgaa gtcgtccact tcgtatccac tgcgtgatag tgcctttgtc
     4081 taccttcaga tacgacgctg cctcttcacg agtgaagatt tcttcttcca cctggaatct
     4141 ccatttattg gattggtatt attgcggtag gtctggatat cattgagcaa tgaacaggcc
     4201 tcatcgagtg tgaggcgtta gtccttgcgt agctcgctga ttcttctgta agtctctggt
     4261 gctttgttcc cgtacgtctt catttcagac ttcaacagag caacgagtga atcccattcg
     4321 ttgaggattc ctttgaatgc cggaacgcgc tttgcaacct tgtcgaatga atctctgatt
     4381 tctggaatct gctcaacaag tgcaacgcat cgccggaaat cggctgcgtc atgtggagca
     4441 ccgaagctat gaccatagat attctttttc agtccacatg cgattgaggc aagagttgcg
     4501 ctactgatgc cgacatcgtc agtcgattgc catttcaaaa ccttcatagc caaatctgac
     4561 atttcttgtc tccaataaaa aaccgccatc aggcggcttg gtgttctttc aattcttcaa
     4621 ttcgaatatt ggttacattg ttttcatata tgaataaata aattagcttt tttcgttgcc
     4681 ttcgcgttct ttattaattt tgacaaactc gtttttacca cgctctccaa atgcgtcttt
     4741 agagtcgttg tatccgcaat cgcagcacac ataatcacca gaccatccac gcattgtttt
     4801 ttcttttgca atatttccag aaccgcattt tggacaagac atatcactac ctccaaagca
     4861 tgagtgagat gacaacgtaa cattgattgg agattaacaa tagattgctg atgtaaaaga
     4921 tatgtataag cttcgctttc aaagtggagg ctctggtagc ggcatccagt gggttacatt
     4981 gctaatcaga ccatattcat tagtttgagg atggttgccg ttatcgtctc cgtatttaag
     5041 actctccata aaaccataat gcttattccc attaacgctc acaaagccgt aataagctgg
     5101 tataacgccg atctcacacg taatcagcaa aggaaaacta gttctccaat ttagctcgcc
     5161 gattacaggc attctctcac tacagcttat ccaaccatcc ggagttaccg gaacttgcgg
     5221 aatggctgtc tgctctcgaa cgtcattagg cgcgataggt tctgctgcca actgactggc
     5281 atatttgtta atggtaacga taagctcttg ctcagcctca tccagacaat caccgatacc
     5341 tcgcctgtca ccgtcaaaat catcgaaatc ggcacgaatc ttggcaacct tctggattgc
     5401 ggacaacacc tcactaggaa ttaccggata gttggttgac gtttccgcga tttcacgaaa
     5461 attattggtt gacgaattct tgttttcccg aaagtttccg gactgaagca tggctgcgcg
     5521 ataggcgttc cagccgacag cttttccgtg ttcaaacgcg ctgtcaaagt catcatccat
     5581 ttccatcgca tcaggcacag ataccgtctc tggaggggcg gcgtaaagcg gagttacggt
     5641 aatttcgtac tcgtttatat cctcgtcgct gatatttgcg aaatagcgaa ggatgccttt
     5701 ctctccacga ctacgagcgt cagagtcctg aatgatatac gccacaggct ctgcttccag
     5761 cgatgccagc gcaattcgtg ccagttccat ttgttcgcca cgagtaagcc cgttttcaag
     5821 cggattttta atgaacaatt caatacgttc tttggtaata gtggtcatag ctatttcacc
     5881 ttaatctcaa tatttcgcag ctttagctct actggcaggt ctgactttcc tgttaacgct
     5941 aatgcgagat tttctggagt aatgagagca gttattgttt tccccatcgc cagacgaata
     6001 atcagtcgta tctcgcgatc gtcacatgct cctggtcgaa caattgatat ttgtccgtcc
     6061 atctcactct cctttgatgc gaatgccagc ggcgcggatt gcagcgatga cctcagaaac
     6121 tttgtatgcc attaccgttt ggtaatcatc gtgaaaatct gttcgatgaa gcatgctgct
     6181 acgttccggg agcagtattc cccgctcccc cagttctgct atgcgattct ctgcggattc
     6241 cagcgccgca accaattcgt ctacagttcc ggcagcttgc agtgcgtaat cggtaatagc
     6301 catctcatga tcaatttcag taccgttctc attcgttgag gtgatagcaa aataatcaga
     6361 gtcgatttcg ttatcagcta agtggcgtag cgtatcggca acaagccggc cgttttcgat
     6421 tagcagcttc cctaccgtta gcgcaatatc ctcgttctcc tggtcgcggg atttgatgta
     6481 ttgctgattc cttacccgtt catccagtag tgccagcacg gtttctggtc cggccagaaa
     6541 tttgaaggcg ttgagcgcat caatatccac accgtaatct ttaagttcct gttcacttaa
     6601 caaatcatca gctggcaaca ttaacaggcg ttccattgct ggaattgcac gttccgccgc
     6661 ctcacgcagt gcctgatagt taatttcgct cactggccgc ctcctttgcg aagctgggcg
     6721 gcgaactcgt tagccatcgc tgaatatggg tgctcatgac cgccaacaac cttcatgctg
     6781 gcagcaaaca tctccacgcc ctgcgcccgc acttcatcca ggaaagtgtc ggtggctggg
     6841 gtttctggaa tggcgtctgg aataacctct gagtagacgc gatccattgc cgactcccat
     6901 ccgtagtaac aagccgaata gccgtcgcgc tggtagccgt ggtcttcaac tccacatccc
     6961 atgccctggt catggtattc aggctgattg tcgggattaa tgcagtattc aatgaccgac
     7021 ttcatccccg cattctccgc cgccagcgcc gaaaacttct cgtgtgccaa cttaacagcc
     7081 gcatcagcct gcttaattga ctcaatcgct ttctggtggt cttcgtacag agccgaaatc
     7141 ttggcctccg cttcagcaaa tttacgcacc agatattcag cgtttgtttc gttaaccttt
     7201 aaatcacatg ggatgcattt acctttcaga aaaccatcca tctcaattag tgacatttgt
     7261 ttcatttctt cccactccgc cacatagcat tcagatattt gttgtcatta acagaaccga
     7321 aactctttct cttaagtaat tcctctctcg atggcattgg ctttacgcgt tggcgaataa
     7381 tcatttctgc cggaagaatg ccgggattgt atgcaagtcc tctcatggta aattcctcag
     7441 tcattactga tagcgccata gcgtgagcgg taattacgca ggcgcgggtc aatttcaggg
     7501 aagtgggtat atgtggcttt gcggaatggt cggattgatg tctggtaaat tcgctcgagt
     7561 tcttctttct ctgcaagcca tatacagtgg cgaaattcct tttcctcttt cgtttcctgc
     7621 ggtagcgaca ttatccgatc gtagtttttt ctgaatttat ccagcacctc cgatacggaa
     7681 ttgccggaac agcggcgtgg gtcatccgca ccatacagag gcgctggcat aatggaatcc
     7741 ttatgttgct actttagaag ggaattgaat cgtcgtattc aggatgattt tgatgattgc
     7801 tactttgctg ctgttggctg tttcctgaag ttgcaaatcc aatctttgca ttcagtaatt
     7861 caagagtgat tgattgtcca ttttgcccct gataaacatc aaccctgatg ttttctccgg
     7921 taatttccac aatgccacct tcaacaagaa cactacggta gtaatccgct tgcgctcccg
     7981 gcttggcaaa tacaacggcg ctgtagtttg tccattcttt cttttttgtc tggcgatcgt
     8041 aatactgaac gccagcacgg atgttgaatc cgatattttc cccggcctga aactctcttg
     8101 cgggcttgtt tagtcttaca gtaatcgaat gtgccattaa gcagccgctc cttctaattc
     8161 gtctcgtctg atgttgtaaa tgtcctgcgc tttgtgctgc tccggtgtgc cttcgagcat
     8221 cttccacgct ttggcgaacg cctgttta
//
LOCUS       DAJTQQ010000148         8159 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000148 DAJTQQ010000000
VERSION     DAJTQQ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.148"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..248)
                     /locus_tag="OO309_003756"
     CDS             complement(<1..248)
                     /locus_tag="OO309_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5848737.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAR"
     gene            complement(335..696)
                     /locus_tag="OO309_003757"
                     /pseudo
     CDS             complement(335..696)
                     /locus_tag="OO309_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1035..1589)
                     /locus_tag="OO309_003758"
                     /pseudo
     CDS             complement(1035..1589)
                     /locus_tag="OO309_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1732..1854
                     /locus_tag="OO309_003759"
                     /pseudo
     CDS             1732..1854
                     /locus_tag="OO309_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2116..2682)
                     /gene="ipgB2"
                     /locus_tag="OO309_003760"
     CDS             complement(2116..2682)
                     /gene="ipgB2"
                     /locus_tag="OO309_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR5848738.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2964..3278)
                     /locus_tag="OO309_003761"
     CDS             complement(2964..3278)
                     /locus_tag="OO309_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848739.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3356..3466)
                     /locus_tag="OO309_003762"
                     /pseudo
     CDS             complement(3356..3466)
                     /locus_tag="OO309_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3790..4467)
                     /gene="ospD1"
                     /locus_tag="OO309_003763"
     CDS             complement(3790..4467)
                     /gene="ospD1"
                     /locus_tag="OO309_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR5848740.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4839..4976)
                     /locus_tag="OO309_003764"
                     /pseudo
     CDS             complement(4839..4976)
                     /locus_tag="OO309_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(5058..5356)
                     /locus_tag="OO309_003765"
                     /pseudo
     CDS             complement(5058..5356)
                     /locus_tag="OO309_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5945..6104)
                     /locus_tag="OO309_003766"
                     /pseudo
     CDS             complement(5945..6104)
                     /locus_tag="OO309_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(6108..7337)
                     /locus_tag="OO309_003767"
                     /pseudo
     CDS             complement(6108..7337)
                     /locus_tag="OO309_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(7357..7704)
                     /gene="tnpB"
                     /locus_tag="OO309_003768"
     CDS             complement(7357..7704)
                     /gene="tnpB"
                     /locus_tag="OO309_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5848741.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(7704..8077)
                     /locus_tag="OO309_003769"
                     /pseudo
     CDS             complement(7704..8077)
                     /locus_tag="OO309_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2088 a         1901 c         1797 g         2373 t
ORIGIN      
        1 cgagcgatta ccgttgccgg gattttgtaa ggatgagcat cggcgatgcg ttgacgaata
       61 taatcccggt attcatccag gagtgaagca acagcaggtc gcggcgtata ttttggcggc
      121 tcagattttg cctgcaaata acgtttaacg gtattgcggg agagccccag ttctctggca
      181 atcgcccggc tactcattcc ctgcttgtgc aggattttaa tttccataac tgtctcaaaa
      241 gtgaccataa gctctcctga atcaggagag cagattaccc cctggatctg atttcaggcg
      301 ttgggtgtgg atcactattg caccgttcgt gacagtgttg ttgcgccttg ctgccaacga
      361 agctccactg cccatcaagt tcacagataa gggcaacatc agcgtgagcg actggcgcag
      421 atgttatccg ccggggcggg agctttttaa tgtccgtatg acggtattga tgccgatttt
      481 cagcgtacgg ccagtatcgc gaactccggc accgttgaaa gccatttcag tgatttggtt
      541 tttaactccc ggtttacggg cctcgtaaca gtaagtgagc tgaaacaccc ggtgacagtc
      601 acggcaacgg aatcgatcat gtccttttgg gttctgcccg tgacggtaaa cctgaacgga
      661 ctggcaacgg gggcaatgaa agttaactgt gaccatgaaa gaacctaaaa gcgggtatta
      721 taacccctaa ttcaactaat tggaggcatt gtcattttta atctatggat acacttaaaa
      781 atcctctcac cttacgttaa aaaccttctc atgtttagag ttttttatta tgtttatcat
      841 aatgatatga cgatctgagt ttttaaaatc aagaagaaaa gataagtttt ttaaatgcac
      901 agtgcctgtg gataactttg cgtaaatccg gaggacagat cactctgaag caagacaaca
      961 gcatctgaaa acaatatgct cacgtttcaa ccacagggcg taagtgatcc tacccacgta
     1021 atatggacac aggcctaagc gaggttctgg ttttcaaatt gttccggact gaggccgcca
     1081 caccaactgt gccgtcgcca gcgattgtaa tcacattcga tataattaaa cactgttgcc
     1141 ctcattattt cccggctgat aaagtgttct ccgtggagac attccacttt cagcgaatga
     1201 aagaagcttt ccacgcaggc attatcgtag cagcagccct ttgcgctcat actgccacgc
     1261 agattatgcc acttcagcag cgcctgataa tctgctgaac agtactgact tcctcggtcc
     1321 gagtgaacga taacgttccg gggtctctta cgccgccaca gcgccatctg cagggcatcg
     1381 caggccagtt gcgccgtcag gcgtggtgac atggaccagc caataacggc acgtgaccac
     1441 aggtcaatga ccacagccag atacagccag ccttcatctg tacgtaagta cgtgatgtct
     1501 cccgcccact tctggttcgg accactggcg taaaaatcct gctccagcag attttctgac
     1561 acaggcaggc catgtgcgcg gtaactgacc gtattgctgg gtaagatcaa tttgctcact
     1621 ggtatttact acgtgcatcg aatgagtgat caagagccgt atgacgcgag agtgtcacgt
     1681 acggttccgt aagcagccgg agagtgaaac tctctccggc tgactcgact cgcaggttcc
     1741 gacagcggtg acgaacatgc agcggtgttg ttctcgctga tcggcacatg ccgtctgaac
     1801 aatgtggagc cagaaaaatg gctgcgttac gttattgaac atatccagga ctgaccggca
     1861 aaccgggtac gcgatctgtt gccttggaaa gttgatctga cttctcagta aatatcaata
     1921 tgattctgac gagccgctta cctttgagca gagaaagggg agagggatcc cacatccatg
     1981 agctaggaaa atacaaaaac tggctataca acaattcaat atattaggac ttacttgcga
     2041 ggagaaatat agcaccgctc aggcaattcc tgggtattta cttttcttag tttctttact
     2101 cgggatgttc tgtcatcaga aaggcgattc taaatttgta atatagtcac tcatattccc
     2161 acgtaatttc tttagatcaa tatattgcac atttttctca aaaaactgat cggaaataag
     2221 atttgcaaca ttgcttgttg tatcattttt aacgcttcta ttcatccctt cacaaagttc
     2281 gtccattttc tttttgaaat aactgcttcc tgatacaaga tgatttatgg aactttgtgc
     2341 agcattgatg tctagagaaa tgcgataatc tttctcaacc tgtttaaata tctgtttacg
     2401 gagattatcg ccgatttttc ggctgtgttc tgcacaaaac ttatcaatac attggtttac
     2461 cacccgggat acgtagactt ttttttcatc atttttccaa tctgtaatat tttcaccgat
     2521 ttgttctgaa acagagctca aaatactagt atttattttg gttgatgata tttttactat
     2581 ccttttcccc attgtacagc ggataatctc atccaccttt cctgaaaaat atcttttgtg
     2641 agataaactg attccaaaat tattaaaaga tgttccaagc attataatca ccttgtcatg
     2701 taaaatagta acattgtcat tattttagct gattcaaaca attctgtaaa tgctttgagt
     2761 tctttactcc cttggctcaa tgatggaccg tctggaatcc cagagactaa actctctgag
     2821 aaagagagta tgaaaaaact tggggcagtc cctaaatgaa gggaccaaca gcggtaaaat
     2881 aaccggcatc attcataatg atgccctgtt agttgtagtt atgaagattt acgtcattaa
     2941 aattgctgtt cgtagtgtca gtcctatggt ctggcatcag ctgaggatcg tagccgatac
     3001 atcactggct tcacgacatt cgtgttgagg ctattcatga aaactccacg ctgaaaacgc
     3061 cgttttgtat aaacggtcac ggtatgcccg gagccactgt tgctgataaa ttcgataaaa
     3121 ccttggcgtt tcttgaagct atcgttaatg cggataatga aacaacgatt ggtgagattc
     3181 gctcttttgc cgacgccctg gacgctgtcc ggttcaatcg caacaaaatc aaccggcagc
     3241 tgagcaaacc caaccttgcc tctctggcgc tggagcatga agtgatttgg ctgggacgta
     3301 gccgctgaca gcttctataa aagtatgatc ctacccacgt aatatgaaca caggcttaag
     3361 tgaggttctg gttttcaaat tgttcaggac tgagacctcc acaccaactg tgccgtcgtc
     3421 agcgattgta atcgcacttg atataattaa acactgtata tttcatttgt ggagtctcca
     3481 gtctgagagg gctttatgca ttccgtattg ggcactatga tgccagaaat ctgcaaggct
     3541 ccacaccttc ctgtatttcc cttcctctat cacagtgcac tttacttaat agggtgggat
     3601 gtgttcatta catttattgt gttttttatg attgtttttg cgtccagtgt cgttcgtcat
     3661 gggaaactgg ttctatgggt agtatcgctc agtctagtta actgtttgtt tctttttaga
     3721 aattaagcag atcacacaat tcggggagag atccttctaa gtgcaattct ttgcagctat
     3781 ttagcacact taaaacaact cttgtatttt gtcagaaata atattaaaca tgtcttgttc
     3841 attattgtta agtctgtctg ctatttctgt aagttttatc ccactatgat ggcatatatt
     3901 tatatatgcc atcatagcct cgatattccc tttccctgcg gccagaaata atcctggtaa
     3961 cccctcatta tctcttacat ccaatagttt aatcgtttct tttggtgata aatcaaaagt
     4021 tttaataaaa tctccataag cttgaatcgc agcggaatga ccttcttgaa gagctatgaa
     4081 caaagcagat tccccagcat tatttcgtgc tgcaagaact tctagttttt cctctttggg
     4141 tatttcggct gccaacagcg attcaccgta tttacgcgct gcatcggcat ttccatgcca
     4201 aatcaatgaa aacaaattat ggcggttata ctccttatta ttctttgtcg gaatggatat
     4261 gttctgtatt ttggccaatt ttctgaaaga aacaccacta tatccccccc ctgatttttc
     4321 ttcattgatg gcgtgggata tagcggtatt tgtcacgtta atgatattat ttttcattcc
     4381 tttattttca ttagcagtat tgctgcctat tattaagtgc atatttttgt tgtttgctgg
     4441 atgtaatcca tagttattta ttgacatttt tgttcatcct ttttgctaaa aaaaaacaat
     4501 aacctatttt tgtaatgtaa tagatattag tctattgcta atctaaaaaa accattgcca
     4561 cattgaatgg ctatattcac caataaattc tttcgatttc gtatattgac atatcgtaga
     4621 tatatcttat ccctaagatt caataatcga gtccgtttac cattctgtgc aagcgccttc
     4681 actcagaagt gaccgtcaag gcgatcacca aacttgataa taaagcagct cattgccata
     4741 cgccagtcct taaggggcgt tgtccatttc tgtgatgctg cctagattta actgccagct
     4801 ccggcttatc tacatctaaa tatgtgagcc taataccgct acactttgtc cagactatgt
     4861 ttttctccgg gaagtgggct aaccgtctcc ataaagtgac gaactttttt caacagtggc
     4921 catattgaac ggcactgatg attacgggtt attgtctcat gaagagccgg ccacatcact
     4981 gacagcagtg acttcaggcc ttcagcacca gacagcctga accagttaat ccatcgcccg
     5041 acaaatttag taatctaggg ctggtttttc ttcagctgtt gcggttcgaa agtgccatta
     5101 cgatcacgca gagtacggat ttccagaggt tcatctccag tagtgataat cttcgttaag
     5161 caaccattgc gggaattgct gccagatcta agctgatttt tctcgcaccc aagatgatga
     5221 gtcatattag cattgagtac ggtttcattt tcagcatcca atcaaactga ctgagatctt
     5281 caggattttt gagacttttg gctagttcat tagctaaagc ccgcaactta ttagatgtca
     5341 actgtttttc attcataaat tcaccttcat ttgatatcaa attaaatagt tcaacatcag
     5401 agaattatac aaacttatgt acaggctcct atgatcttcg aatcctgaag attatagtga
     5461 aaataagttt tcacgttgaa atcccacata gttagtatat gataaataat catggatgac
     5521 ctgattaacc tcttggggta gtgcttttat agaagaataa atattctact aatttaatac
     5581 atcacctaaa atagtattgt tttttatggt tggttgaagg tcgtggtgat gatgttagtc
     5641 cttttgagaa tattccagtg ccagatctta cttataataa aggggcttgg cagtttggat
     5701 aaactattgt tcttattcca ctaatacaaa ctttttgtaa agtatattaa atatacttca
     5761 tttcatactg aaatccccgg aagtattcaa tcaaacaatt acaccataaa aggaagaggg
     5821 ggccctaatt aagttgtcaa gcatgtttat gatacctgca gggtataaat tttctggctg
     5881 caagcagaca catataaaac gtctttattg atcctaccca cataatatgg acacagtcgt
     5941 aagtgaggct ctggttttca aattgttccg gactgagacc accacaccaa cggtgccgtc
     6001 gccagcgatt gtaatcgcac tcgatataac taaacactgt tgcccgcact atttcccgat
     6061 ggataatagt gttctccgtg gagacattcc attttcagcg aatgaatttt catctgttgc
     6121 tgtatccagt catacagtga ctgcatcagt ggcgcggctc tggcttttct tgccgccaga
     6181 cgctgttctg ctgaacatcc ccggacttct gcctcgatgg catacagttc accgatacgc
     6241 tgcagggctt ccgtggtgat gtcggttggc actcttgcat gcacatcgtg gatttttctc
     6301 cggacatgag ccatacatgc ggcttccgtt attctgccgg attcgtacaa cgcccggtaa
     6361 ccaccgtaag catcggcctg aagcactccg ctgtaaccgg ccagatgatt ctgtggatgg
     6421 atacctttcc ggtccggtga gtacgcgaac cagaccgccg gaggcatttc cgaaccggcg
     6481 ttacggtcat cacgaacgta gacccacagc cgggcagtcc ttgttttacc gctgcccggc
     6541 tcctggaccg gaacggggat atcatcggca tggactttac cgggcatcag cacatactgg
     6601 cgcaggatgt catacagcgg ttccagcagt tcagcaacag cacctgtcca gcgccccagt
     6661 gtggcacggc tcagctccac gccctgacga cggtatattt ctgactggcg gtataacggc
     6721 agatagtctg catattttcc agcaacaaca tgggccagaa gccccgctcc ggcataactg
     6781 cgtgcaatgg gttttgaagg caccggtgcc tgcacgatat ggtcacaccg gcaacaggcc
     6841 agtttcggac gttgtgtttc gataacctta aaggcgctgc tgataagctc cagctgctct
     6901 gacacatcac atcccagaga actgagttcg ccaccacagg ccgggcagca ttcctcttcc
     6961 ggacggatga cccgggtttc acggggaagt gaggctggta acggtttacg ggctgaagac
     7021 tggcgcaggg gagatggcag taccgggtca tattgctcac ccagcgtttc cgccatttct
     7081 tcctgaagtg cgctgattcg ctcctgtgct tccagtatct gccgttcggt ttttgcgcga
     7141 agtttttctg agcttttacc gaactgcatg cgctgcagtt tcgcaaccag cgccttcagc
     7201 cggttgattt cggaagcata agccgccacc cgctgtgaga gcaggcggtt gtatttcgcc
     7261 atctggcgga tggtgtcctg ttgcgtctgc aacagtgccc gcaggcgggc attctcatga
     7321 gcaagtgagg tgtccatatc ctcactttac aacgggttat atgcggatgc cagcgcgttc
     7381 cgttcgtttc gggtgtttcc agtcgatgcc ctcaagaagc atggataact gggccggagt
     7441 aaggtgcacc ttgccgtcac gggtgaccgg ccagacgaag cggccccgct ccaggcgtct
     7501 ggtaaagagg cacagtccgt cactgtcagc ccacaacact tttatctggt caccccggcg
     7561 tccgcggaag atgaacagat gcccggagaa cgggtcatcc ttcaggacgt tctgaacttt
     7621 tgagaccagg ccgttaaagc catttcgcat atcggtgata cctgcaacca gccagatacg
     7681 cgaacctgca gggaaagata tcatcagtgg ctgctccctt ttatttcgcg gataagtatc
     7741 tgtaataacg ccggcgtcag tttaccttta agcctgagag ttccggccgg cagaaccagc
     7801 tcacagcaca gactgtcgga cggtgtattt atctgctctg gttcctgtgc cggagccggg
     7861 attttattat ccggctccgg cgttaacgtc acgggaagca gtgcaggcat atttttccgg
     7921 aaggcagcag gccacctttc cggtattgat ggcgccagtt gaagagcagg ttatcgttga
     7981 ttccgttttc acgggcgatc tgcgccacac aggctccggg ctgcagtgac tgctccacta
     8041 aagcgatttt aaattcataa ggaaaattag gccgccggtg taatgccgat cagttaagga
     8101 tcagttgacc gatccagtgg ctgtgtaaga atccggaaac gctcacttgt ttccggatt
//
LOCUS       DAJTQQ010000149         8126 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000149 DAJTQQ010000000
VERSION     DAJTQQ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.149"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..294
                     /locus_tag="OO309_003770"
     CDS             <1..294
                     /locus_tag="OO309_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCR5848742.1"
                     /translation="RWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMALSSTL
                     DSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            266..2176
                     /locus_tag="OO309_003771"
     CDS             266..2176
                     /locus_tag="OO309_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR5848743.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLQFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTCDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TRVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGIFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVQLHPARMQLHNR
                     PLKNWQRSCPEE"
     gene            2177..2380
                     /locus_tag="OO309_003772"
     CDS             2177..2380
                     /locus_tag="OO309_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5848744.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2377..3970
                     /locus_tag="OO309_003773"
                     /pseudo
     CDS             2377..3970
                     /locus_tag="OO309_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3960..5378
                     /locus_tag="OO309_003774"
     CDS             3960..5378
                     /locus_tag="OO309_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR5848745.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATDAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPLAAMPEMTVEKARPILAASPQANAGPSLRDQIMAL
                     DEAKGAEAQAEKLAACPGMTVENARAVLAAASGRAEPVSASTTAMFEHFMANHSPAAV
                     QGGVPQTSADGDADVKMLMAMP"
     gene            5415..5762
                     /locus_tag="OO309_003775"
     CDS             5415..5762
                     /locus_tag="OO309_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR5848746.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5820..6848
                     /locus_tag="OO309_003776"
     CDS             5820..6848
                     /locus_tag="OO309_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR5848747.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6900..7286
                     /locus_tag="OO309_003777"
     CDS             6900..7286
                     /locus_tag="OO309_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5848748.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7298..7675
                     /locus_tag="OO309_003778"
     CDS             7298..7675
                     /locus_tag="OO309_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5848749.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7662..>8126
                     /locus_tag="OO309_003779"
     CDS             7662..>8126
                     /locus_tag="OO309_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5848750.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIP"
BASE COUNT         1877 a         1910 c         2511 g         1828 t
ORIGIN      
        1 agatgggaac tgaccgcaga acaggctgtc gcacagcagt taaaaaatca ggtgtcaaaa
       61 ggaaatctta tcgataccgg attttgtatt tttgccctta gtaagctggc gatggcttta
      121 tccagtacgc ttgactccat tcctttgtcc atgcaacgac agttcccgga tttaacgccg
      181 cgtcatattg atcatctgaa aatccttatt gctaagggag cgaatcagtg cgcgcgtgcg
      241 ggggataaat taccggattt actcgatgaa tatatcagaa caacaactga gtaatatgat
      301 ggtggcagtc actatcgcac tgcagccact ggtcagggta ttgccggtga cggctgtaga
      361 atgggccgat caaaattatt acctgcctaa agaatcgtca tacggtgagg gggaatggaa
      421 aacgctgcag tttcaggtcg ccatcatgaa ctgcatgggc aatgacgaga ttcgtacagt
      481 taacctgatt aaatctgccc gtgttggtta tacaaaaatg gtgcttggtg tgatcgggta
      541 ttttattgag cacaaatccc gcaacactct tctttttcag ccgacggact cagctgctga
      601 agattttatg aagtctcacg tggaagcaac aattcgtgat gttccttgcc tgaaaaaact
      661 ttctccctgg ctgggccgga aacaccggga taatacgctc acactgaagc gtttttcctc
      721 tggtgtgggg ttctggtgtt tgggtggtgc tgctgccaaa aactaccgtg aaaaatctgt
      781 cgatgtggtc tgctatgacg aactctcctc gtttgaaccg gatgtggaaa aagaaggttc
      841 gccaacactg cttggcgata aacgtattga aggttcggta tggccaaaat ccatacgcgg
      901 ttcaacgcca aaaattaaag gctcctgtca gattgaaaaa gctgcgaatg aatctgcgca
      961 tttcatgcgg ttttatgtcc cttgccctca ttgcggggag gagcagtatc tgaagtttgg
     1021 cgatgatgcg acgtcgtttg gcctgaaatg ggagaagggt aaaccggaaa cggtgtatta
     1081 cctgtgtgaa cataatggct gcgtgatccg tcagtctgaa cttgatcaga ccggggggcg
     1141 ctggatttgt gacaataccg ggatgtggac gtgtgacggt ctgacgtttt tcagtgcttc
     1201 gggtaatgaa attccgccgc cgcgctccat cactttccat atatggacgg cgtacagtcc
     1261 gttcaccacc agggtacaga tagtctgtga ctggctggat gcactgaaag atcccaacgg
     1321 cgtgaaaact tttgtgaaca ccacgctggg cgagacctgg gaagaggccg tgggcgaaaa
     1381 actcgatcac caggtactga tggataaggt tgtgccttac acggcaacgg tgcctgtccg
     1441 ggtggtttat ctgacggcgg gcattgactc gcagcgaaac cgttttgaga tgtatgtctg
     1501 ggggtgggct ccgggagagg aagcctttct ggtggataaa atcatcatca tgggacgtcc
     1561 tgatgaggaa gagacgctgt tacgtgtgga tgcggcgatc aataaaaaat accgccatgc
     1621 agacggtacc gaaatgacca tttcccgtgt ctgctgggat atcgggggga tcgatggcga
     1681 aatcgtgtat cagaggtcaa aaaaacacgg tattttccgg gtactgccgg taaaaggtgc
     1741 ctctgtctat ggcaagccgg tgatcaccat gccgaaaacc cgcaatcagc ggggtgtgta
     1801 tctgtgtgag gtggggacgg ataccgcaaa agaaattctc tatgcccgaa tgaaggccga
     1861 tcccacccct gtgaatgagg ccacatcgta tgccatccgc tttcctgata atccggagat
     1921 tttttcgcag acagaggcac aacaactggt ggcggaggaa ctggtggaaa agtgggaaaa
     1981 aggaaagatg cgtctgttgt gggataacaa aaagcggcgt aacgaagcgc tggactgcct
     2041 ggtgtatgcc tatgcggcat tacgtgtgtc cgtgcaacgc tggcaacttg atctggctgt
     2101 gcagttgcat cccgcaagga tgcaactgca caaccgaccc ttgaagaact ggcagcgaag
     2161 ctgtccggag gagtgaatgg ttacagtcgc tgaactgcag gcgctgcgtc aggcgcgcct
     2221 tgatttatta accggtaaac gggttgtgtc ggtacagaaa gacggtcgca ggatcgaata
     2281 tacggcagcg tccctgaatg agcttaaccg tgcgatcaac gatgcggagt cggttctggg
     2341 tacgacccga cgccgccgac cgctgggagt gaggttatga aacgaacgcc tgtcctgatt
     2401 gatgtgaacg gcgttccgct tcgtgagagt ctcagctaca acggaggcgg cgcaggattt
     2461 ggcgggcaaa tggcagagtg gttaccaccg gcgcagagtg ccgatgcggc cctgttgcct
     2521 gcattgcgtc tggggaatgc ccgggcagat gatctggtgc gcaataacgg tatcgcggct
     2581 aatgcggtgg ccctgcataa ggatcatatc gtcgggcaca tgtttcttat cagctaccgt
     2641 ctgaactggc gttggctggg gatgcgggag accacggcaa aaagttttgt cggggaggtg
     2701 gaagcagcct ggtcggaata cgcagaagga atgtttggcg agatcgacgt ggaagggaaa
     2761 cgcacgttta cggaatttat ccgtgaaggg gtgggcgtgc atgcctttaa cggcgaaatc
     2821 tttgtgcagc cggtctggga tacggaaacc acgcagttat tccgtacgcg ttttaaagcc
     2881 gtgagtccga aacgggtgga cacgccagga cacggtatgg ggaaccactt tctgcgggcc
     2941 ggggttgagg tcgatcggta tgggcgggcg attgcgtacc atatctgtga ggatgatttt
     3001 ccgttctctg gtgccggacg ctgggagcgg atcccgcgtg agcttcccac cgggcgtccg
     3061 gccatgctgc atatttttga gccggtggag gacgggcaga cccgtggggc taatcagttt
     3121 tacagcgtaa tggagcggat gaagatgctg gactccctgc aggccacgca gcttcagtcg
     3181 gccattgtga aggcgatgta tgcagccaca atcgaaagtg aacttgatac cgaaaaggca
     3241 tttgaatata tcgcgggggc accgcaaggt cagcaggata atccgcttat taatattctg
     3301 gagaagttca cccgctggta tgacacgaat aacgtgacgc tgggcggtgt taaaattccg
     3361 cacctttttt cctggcgatg cgctgaacct gcagacagcg caggattcag acaatggatt
     3421 ttcggcgctt gaacaggcgc tgctgcggta tattgcagcc ggtcttggcg tttcctacga
     3481 acagttgtcc cgtgattact cgaaggtcag ttactcaagt gcccgcgcct ccgccaatga
     3541 gtcgtggcgc tattttatgg ggcggcgaaa atttattgcg gcccggctgg ccacgcagat
     3601 gttttcctgc tggctggaag aggcgcttct tcgggggatt attcgtccgc cacgggcgcg
     3661 ttttgatttt taccaggcgc gttcagcctg gtcacgggca gagtggatta gctccggaag
     3721 aatggccatt gacgggctca aggaggtcca ggaatcggtg atgcgcattg aggccggact
     3781 gagcacgtat gagaaagaac tggcactgat gggcgaagat tatcaggaca ttttccgcca
     3841 gcaggtcagg gaatccgcag agcgcgaaaa agccggactc tcacgtccgg tgtggatagc
     3901 ccaggcgtat cagcagcaga tagcggagag tcgcaggccg gaagaggaga caacatcacg
     3961 tgagacgtaa tctttcacat attatcgctg cggcattcaa tgagccgctg cttctggagc
     4021 ccgcctatgc gcgggttttc ttttgcgcgc tcgggcgtga gatgggcgca gcaagccttt
     4081 cggtaccgca acagcaggta cagcttgacg ctcccgggat gctggctgaa acggacgagt
     4141 acatggccgg aggcaaacga cctgcccgtg tttaccgggt ggtgaacggt attgctgtac
     4201 tgccggtgac cggcacgctg gtacaccggc tgggtggcat gcggccattt tccggaatga
     4261 ctggctatga cggcattgtc gcctgtcttc agcaggcaat ggcggacact caggtacggg
     4321 gcgtactgct ggacattgac agttctggcg ggcaggccac cggcgcgttt gactgcgctg
     4381 acatgattta ccgtctccgg cagctgaagc ctgtctgggc actgtgcaat gacacggcct
     4441 gttcagccgc catgttactg gcatcggcct gctcccgacg gctggttacc cagacatccc
     4501 gtatcggctc cattggggtg atgatgggcc atgtcagcta tgccggtcat ctggcacagg
     4561 ccggtgtgga tatcacgctg atttacgccg gatcgcacaa ggtggatggc aatcagtttg
     4621 aatccctgcc agcaaaggtt cgccaggaca tgcagcagcg cattgatgcg gcgcgccgga
     4681 tgtttgctga aaaagtggcg atgtataccg gtttatctgt ggatgcggtt atgggaacag
     4741 aggccgccgt ttttgaaggt cagtccggta ttgaggccga actggcggat gaattaatca
     4801 atgcgtcgga cgccatcagc gtgatggctg cgacgctgaa cacacacgat acaggaggca
     4861 ctatgccgca attaactgca acggatgctg ccgcgcagga gaaccagcga gtgatgggga
     4921 tcctgacgtg tcaggaagcg aaaggacgtg aacaactggc cacgatgctg gcagggcaac
     4981 agggtatgag cgttgagcag gcccgggcga ttctggccgt ggcggcaccg ctggcggcga
     5041 tgccggagat gacggtggaa aaagcccgtc cgattctggc tgcttcaccg caggcgaatg
     5101 ccggaccctc acttcgtgat cagattatgg ccctggatga ggcaaaaggg gcagaggcgc
     5161 aggctgaaaa actggcggcc tgtccgggga tgaccgtgga gaatgcccgg gctgtactgg
     5221 cggcagcctc aggcagggca gaaccggttt ctgcatccac aaccgccatg tttgaacatt
     5281 tcatggcgaa ccattcaccg gcagcggtac agggtggcgt gccacagacg tcagcagacg
     5341 gtgatgcgga cgtgaaaatg ctcatggcca tgccatgaag tcagtgctga ccatcaatat
     5401 gaggttttta caatatggta acgaaaacca tcactgaaca gcgtgcggaa gtacgtattt
     5461 ttgccggtaa tgatccggcc cataccgcca caggctgcag cgggatttcc tcggcaacac
     5521 cggctctgac gcccctgatg ctggatgagg ataccgggaa actggtggtc tgggacggac
     5581 agaaggcagg ccatgtggtt ggcatactgg tactgccgct tgaaggtaca gagacggtgc
     5641 tgacgtatta caagtcgggg acctttgcga cggaggccat ccactggcct gagagagtgg
     5701 atacacacaa aaaggcgaat gcctttgccg gcagtgccct gagtcacgca gcgctgccgt
     5761 aacattttat caggtcactg cggtagcctg actgatttct gaatgaaagg aactgattta
     5821 tgggattgtt tacgacccgc cagttactcg gttataccga acaaaaagtt aaatttcgtg
     5881 cgctgtttct ggagctgttt ttccgccgta cggtgaattt ccataccgaa gaggtaatgc
     5941 tggacaaaat taccggaaaa acgccggtgg cggcctatgt ctccccggtt gttgaaggaa
     6001 aggtgttgcg tcaccgtggc ggtgaaactc gtgtactgcg cccgggctac gttaagccga
     6061 aacacgaatt taattaccag caggcggtgg agcgtcttcc cggtgaagat ccggcccagc
     6121 tgaacggccc ggcttaccgt cgcctgcgta tcatcaccga taacctcaaa caggaagagc
     6181 acgccattgt ccaggtggaa gagatgcagg cggtgaatgc cgtgctgtat ggcaaataca
     6241 ccatggaagg agagcagttt gaaactgttg aggtcgattt tggccggtcg gtgggaaata
     6301 acattacgca ggcaggaggg acggagtgga gcgctcagga ccgcgatacc tttgatccga
     6361 cgcatgatct ggatgcgtac tgtgattttg cttccggcac catcaatatt gccattatgg
     6421 atggtaaggt ctggcgtctg ctgaatggct ttaagctgtt ccgcgaaaaa ctggataccc
     6481 gtcgtggctc aaattcacag ctggaaacgg cagtgaaaga cctgggggcg gtggtgtcct
     6541 tcaaagggta ttatggtgat ctggcaatcg tggtggcaaa aacgtcctat gtggcagagg
     6601 acggtaccga aaaacgttat ctgccggacg gcacactggt actgggaaat acggccgcag
     6661 agggtattcg ttgctacggt gccattcagg atgcgcaggc attgtctgaa ggcgtggtgg
     6721 cgtcttcccg ttatccgaaa cactggctga ccgtgggtga tccggcgcgt gaattcacca
     6781 tgacgcagtc cgcgccgctg atggtgctgc cggatccgga tgagtttgtg gtggtgcagg
     6841 tgaaataatc cgtgagcggg agctaaatgc tcccgttttt cattcaatca ggagcgggta
     6901 tggctacgaa agaagaaaac ctgaaacgtc tttgtgagct ggctgagagg ctggggcgtg
     6961 aaccggatgt ctccggtagt gcggcagata ttgcgcagcg agtggcggaa ctggaggaag
     7021 agcttggaga tgcgggggag gtcgcagagc cggatacgta ctcaccatcg aaagatgatc
     7081 cgaccgcgca taaaggcgag tcagttcctg aacagccagg cattgttacc ggagacgaaa
     7141 cggggcaggt tacggtggtg gcactggcga cactgcatac aaaatgtaat ggttcagtga
     7201 tatttgtttc accgggaacg tcgtttcgtg tttctgctga ggtggctgcc agcatggcgg
     7261 caggtggtct ggcaaaaagg cagtaacagg agatgtcgtg gacggtttcg ataatctgtt
     7321 tgatgctgcg cttgccggtg tcaatgaagt cattctccgt gatatgggga tcagcgccgt
     7381 cattacgtcc ggagaacttg aggggacaca cctgacaggg gtttttgatg atcccgaaag
     7441 tatttctttt gttgccgggg ggatccggct tgaggattcc tcaccgtgcc tgtttgtgaa
     7501 aacggcagat atatcgcagt tacgccgtca ggatacgtta actatcgggg atgattcttt
     7561 ctttgtggat cgtattactc cggatgacgg gggatgctgt tatatccggt taaggcgggg
     7621 cagtcctgcc ccacagaata atgccagaat gaggcgatat gatgaaggga cttgagaatg
     7681 ccatccgcaa tctgaacagc ctcgataccc gcatggtgcc acagaccagc gcatgggcgg
     7741 taaaccgtgt ggcggcaaag attgtttctg tcgccacacg gcaggttgcg cagaataccg
     7801 ttgccgggga taaccaggtg aaaggtattc ccctgaaaac ggtcagggag cgtgtcaggc
     7861 tgcttaaagc cagcccctca ggaaaaatgt atgcccgcat gcgtgttaac cggggtaacc
     7921 tgcccgccat taagctgggt accgcacagg tcagactggc ccgctcccgg catggcagca
     7981 actcacgtca tcgtggcagc gtactgaaag tggggaaata ccttttccgg gatgcgttta
     8041 ttcagcaact ggcgaatggt cgctggcatg tgatgcggcg tattgacggc aaaaatcgtt
     8101 accccattga tgtggtgaaa atcccg
//
LOCUS       DAJTQQ010000150         8057 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000150 DAJTQQ010000000
VERSION     DAJTQQ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8057
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.150"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(14..707)
                     /gene="ospZ"
                     /locus_tag="OO309_003780"
                     /pseudo
     CDS             complement(14..707)
                     /gene="ospZ"
                     /locus_tag="OO309_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            763..915
                     /locus_tag="OO309_003781"
                     /pseudo
     CDS             763..915
                     /locus_tag="OO309_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2296..3243
                     /gene="icsP"
                     /locus_tag="OO309_003782"
     CDS             2296..3243
                     /gene="icsP"
                     /locus_tag="OO309_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR5848751.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3805..4177)
                     /locus_tag="OO309_003783"
                     /pseudo
     CDS             complement(3805..4177)
                     /locus_tag="OO309_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4421..4540
                     /locus_tag="OO309_003784"
                     /pseudo
     CDS             4421..4540
                     /locus_tag="OO309_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4790..5656
                     /gene="ospB"
                     /locus_tag="OO309_003785"
     CDS             4790..5656
                     /gene="ospB"
                     /locus_tag="OO309_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR5848752.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5985..6725
                     /gene="phoN2"
                     /locus_tag="OO309_003786"
     CDS             5985..6725
                     /gene="phoN2"
                     /locus_tag="OO309_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR5848753.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(7085..>8057)
                     /gene="ospC4"
                     /locus_tag="OO309_003787"
     CDS             complement(7085..>8057)
                     /gene="ospC4"
                     /locus_tag="OO309_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_156647404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR5848754.1"
                     /translation="DKIQSENIQTHKNTYSEDIKEISNHDFVFFGVEISNHQEKLPLN
                     KTHHTVDFGANAYIIDHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVS
                     RYVHGNQGKTDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPVSLDRII
                     NFVFQLEYHIPRMLSTDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYS
                     LGNKKADIALYLLSKFNFTKQDVAEMEKMKNNRYCNLYDVEYLLSKDGANYKVLEYFI
                     NNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFFIKKWSGIRQTI"
BASE COUNT         2526 a         1502 c         1506 g         2523 t
ORIGIN      
        1 caattaatat acattaagta acaggcattc gagccttgat ttgagaaagc ctgtcattca
       61 tttgttttat gtagctatct atttcctctg atgcataact aaaaagttct cccatttcac
      121 gagcaataat aatctcagat ttaatagact ttaatctctg gcgactcatc tcttaacact
      181 tgcataatct ctctttttat atcatatagt tctataaacc gatttgcccg tagttctcct
      241 tgggaagaat catttctgaa tacaacaatt atatacaaaa aatcactatt ttctttagag
      301 aagatcttac aactaatctc tgggagctca cataatgcat gtaagtcagg atggtacaaa
      361 ctatccacag aaaattgccc cataggacgt tttccagata taactgtcaa ccagcttcct
      421 tccggtgtac tggcttcggc tctggaaata ggctgaaaat cttccggaca tggagaatac
      481 attgtcttca tataattaag tcgtctggat ttctcattaa taaactcctc tatttttaat
      541 tgtgccatac ttctggttat accagagata tacttcttgt catatatgtt tggtgattca
      601 ttaaagtaac catgttcaac tctaggataa atatttctca ctatatattc agtatttgca
      661 gtatttattg gaaatacgtt cttaatattc ttgatgggac taatcatttt aatctctata
      721 ctcaaatata aacattacca tgaacaatat aattgttcct aagtggaatg tctccacgga
      781 gaacacttta tctatcggga aatagtgcgg gcaacagtgt ttaattatat cgagtgcaat
      841 tacaatcgct ggcgacggca ccgttggtgt ggcggcctca gtccggaaca atttgaaaac
      901 cagaacctcg cttaggcctg tgtccatatt acgtgggtag gctcacagag cctcacttac
      961 gactgcgtcc atattatgtg ggtaggatca ataaagacgt tttatatgtg tctgcttgca
     1021 gccagaaaat ttataccctg caggtatcat aaacatgctt gataacttaa ttggggctcc
     1081 ctcttccttt tatggtgtaa ttgtttgatt gaatacttcc ggggatttca gtatgaaatg
     1141 aagtatattt aatatacttt acaaaaagtt tgtattagtg gaataaggac aatagcttat
     1201 ccaaactgcc aagcctcttt attataagta agatctggca ctggaatatt ctcaaaagga
     1261 ctaacatcat caccacgacc ttcaaccaac cattttattg catttaaatc ccagaatagt
     1321 tcagtagtgg tcaatttggg aaacgcacaa tattgtacgg ttacgttatc gcaatagagt
     1381 gaactctcgc cctatacggc gttttcaagg attagtcctt aatcggacaa ccacattaaa
     1441 gcataataaa taattaagct ttaataatat actatagata cagttctatt ataaaaaata
     1501 ctattttagg tgatgcatta aattagtaga atatttattc ttttataaaa gcaccaatga
     1561 attgtccaag taaacatgag ataaaatgtg tagaggataa ctgagtttgg tcactttaac
     1621 tgtattagtc gcagtctgac tctcgacttt aaaaggatgg gaattacctg tactgatagt
     1681 gagaaagctt tccattgaaa taagtcaaat aaaagttgat ggcttcagtg ctaaagtgag
     1741 gtgcccgcta gccgtattca ctcgaatcgg aattcaatgt attgcttctg ccatttccat
     1801 aatatttcac gggaaaaaca aaatctgaaa aactatactt aagtgttgta ttgagtcagt
     1861 tgagatacaa atacatattt atcagcacac gttacgtcaa atacaggagg ttgtcatgtt
     1921 ggtactgaaa ggcacgttgg ctattgcagc cgtgatgaca atttattgtc ttatcgttgt
     1981 tcttatggaa cgcttttctg attgatttca tattggcgag agaacaagaa ggtgagtaga
     2041 attgtcgcgg aatcctgaaa ctatcagcct caggtatgaa caccaaaagg taggtagaga
     2101 aatctatggc ccccgttact ttattcgcac aatgaggtat ttttttattt ttacaaaagt
     2161 tcaataaata ttataataaa agatatgttc ttggtaacat aaacaaatat ttaattaaaa
     2221 taatgcttgg cattgacgtt tatcttataa agtaagaaga tcatggacat ttcaaccaaa
     2281 aaagtagagt tctcgatgaa attaaaattc tttgtacttg cactttgtgt acctgcgatc
     2341 tttactacac atgctaccac taactatcca cttttcatac cggacaacat cagtacggat
     2401 attagtctag gatctctgag tggcaaaaca aaagaacgcg tttatcatcc caaggaaggg
     2461 gggagaaaaa ttagtcaact ggactggaaa tacagtaatg cgactattgt tagaggtggc
     2521 atcgattgga agctaattcc aaaagtgtct ttcggagttt ccggttggac tactttaggt
     2581 aaccagaaag caagcatggt tgataaagac tggaacaatt ccaatacccc tcaggtatgg
     2641 accgaccaga gctggcatcc caatacgcat ctccgtgatg ctaacgaatt cgagctgaat
     2701 cttaaaggtt ggttattaaa taatttggat tatcgacttg gactaatagc aggttaccaa
     2761 gagagtcgtt acagttttaa tgcaatggga gggagttata tttatagtga aaacgggggg
     2821 agcaggaata aaaaaggggc acatcctagt ggtgaaagaa caataggtta caaacagctc
     2881 ttcaaaatac cttatattgg attaactgct aattaccgcc atgagaattt tgagtttgga
     2941 gcagaactga aatatagtgg ttgggttctt tcatctgata cagataaaca ctatcagact
     3001 gagacaattt ttaaagatga aataaaaaac caaaattact gctctgttgc tgcgaatatt
     3061 ggatactatg tcacccccag tgcaaaattt tatatagaag gctccagaaa ttacatttct
     3121 aataaaaaag gtgatacatc tctttatgag caaagtacca atatatctgg caccattaaa
     3181 aatagtgcaa gtattgaata tattggtttt ctcacttccg caggtataaa gtatattttt
     3241 tgaattggtt caccgagttg attacgtttt catggctaaa aattgccgcc cattgtgagt
     3301 catattcgcc ctgactttcc agaaccatac gaactgcccg ttgacggact tcgggggaaa
     3361 aacgtgtatt tttagtcatt ctgtttacct ctttctcagg gcgtttagtc tccaggattt
     3421 ccggggcggt tcaaaaaact cttcgttgat tactgtggac ttactgttgg cattaccgac
     3481 cctgagaccg gagaaataag aaccgcttag atcatcgtgg ctgttctcgg ggtatcaagt
     3541 tacacatggg cagaggctac ccaaggtcta ctattatcgc ctgaaagcgc tgatggaaca
     3601 gtgctatcag ggacatgctg atgaaaatac ctaaataatc agccagcact ctatctttcc
     3661 aaatccacag catagcaaag agagcaaaag agcctgtaaa ttcagaaatt aaggaaacgc
     3721 ctcttggtgt acataatcta tcttatgtta aatgaaatct aatttcttga ttatatgctg
     3781 gatcgttcgt gggtggcttt gcccctatat ttccagacac ctgttatcat tggcccgctg
     3841 ccgcagatat tcccgtggcg agcgataatc cagcgcacta tgcggatgcc attcgttata
     3901 atgctcgaat gcctctgcaa ggttctttgc tgccgttaac ccgtcaggtt tgggcatgat
     3961 actgatgtag tcacacttta ttgttttcac gaagctcttt gctattctgt tactctccgg
     4021 actccgcacc gccgtgttct tcggttcaac aaagtcccaa catccgggtg aactggcgtg
     4081 tttcattagc tcggtagcat gaaccattat ccgtcagtca cttcactgga gacgccggaa
     4141 gctcgttgcc gaagcggtgt tccaccgctc ccagcatgtc gccaacggga gagcatctac
     4201 ggtctgattg aactgtttat tcaaattgtc caccgtctgt cagtacgctc tgaacgtcgg
     4261 ctggttaaaa cactgctcgc tgattttcag agggttcacg gtaaaacagc acttttattc
     4321 cggattgcgg aagcggctct gaataatccg gatgggctgg ttaaagaggt tgtgtaccat
     4381 ctgcatattg ttgaaccaga tcgcagcggt aagagaagtt catcatactt ggcgcagtta
     4441 cgcgatgttt ctgctagagg tgatgccgta aagaacggca gaacattacc ggaacaagat
     4501 agcggtctac ctgcactggt atcagacccg gggcttcccc ggatgatctc cacggtactt
     4561 taggtactgt gtctatatta tgtaggtagg atcaattaac tttgtagttg aaattctttc
     4621 attgaaataa tgaataaaaa agacaaaccg cattaaacaa tgccagaata taattgcatt
     4681 tttgttccgt ttttttaaat catagtatac aaagaagctt gcatactata tgaaaatata
     4741 acacccttta ctatgatgtt tataaaacaa tatatggagt catgtaggta tgaatttaga
     4801 tggtgttaga ccatactgta gaatagtcaa taaaaagaat gaaagcatat cagatattgc
     4861 atttgcacat ataataaaaa gggtaaaaaa ttcatcatgt actcacccaa aagcagcatt
     4921 ggttttttta ggagagaaag gtttttgtga tagcaatgat gttctatcta ttatgggaca
     4981 acaaatacca agagtattta agaacaagat gttatatgat tatgttttta aaaatgaaaa
     5041 aagtaaaaat gattttctaa aaatggctga atcatggcta ccacagagtg aaccaatagt
     5101 aataaataat gatgatgacg cattgaatgc tgctgcttat ttttctgtaa aaaaagcgaa
     5161 aataaaaaca gtaaacgata ctgattttaa agagtataat aaggtttata ttcttgggca
     5221 cggtagtcct ggttctcatc aattaggcct tggttcggaa cttattgatg tacaaacaat
     5281 catttcaaga atgaaagact gtggtattct aaatgtgaaa gatatccgtt ttacttcatg
     5341 cggctccgct gataaagtgg ctcctaaaaa ttttaacaat gcccctgctg aaagtctttc
     5401 ttgtatcctt aactctctgc ctttttttaa ggaaaaagaa tctttgctag agcagataaa
     5461 aaaacacctt gaaaacgatg agtcattgag tgatggtcta aaaatatccg gctatcatgg
     5521 atatggagtt cactatggtc aagagctttt tccctactca cattatcgtt caacttcaat
     5581 tcctgctgat ccggagcata cagtaaaaag aagctctcag aaaaagactt ttattattaa
     5641 taaagaactg gattagtata aaatttttaa cctatagtta attgtttgca tttaagatga
     5701 agttatttta tctttagtag aaaagtgctt ccctatgagg gaaaatcaca aatcatcata
     5761 atacagagac aaaacgatac gaaaaaatag ataaaaaaca tcattctttt accacattat
     5821 tttccgtgaa tatgaaaaat aatgttatta ctttaatata agactatttt ttgtttttcc
     5881 atcactctgt tcaaattttt cagcatgact tgtgtttttt gtaatacagc tcgtttttta
     5941 cagctgacca aaatcatcaa ttaattatgc taaggaaata aattatgaaa accaaaaact
     6001 ttcttctttt ttgtattgct acaaatatga tttttatccc ctcagcaaat gctctgaagg
     6061 cagaaggttt tctcactcaa caaacttcac cagacagttt gtcaatactt ccgccgcctc
     6121 cggcagagaa ttcagtagta tttcaggctg acaaagctca ttatgaattc ggccgctcgc
     6181 tccgggatgc taatcgtgta cgtctcgcta gcgaagatgc atactacgag aattttggtc
     6241 ttgcattttc agatgcttat ggcatggata tttcaaggga aaatacccca atcttatatc
     6301 agttgttaac acaagtacta caggatagcc atgattacgc cgtgcgtaac gccaaagaat
     6361 attataaaag agttcgtcca ttcgttattt ataaagacgc aacctgtaca cctgataaag
     6421 atgagaaaat ggctatcact ggctcttatc cctctggtca tgcatccttt ggttgggcag
     6481 tagcactgat acttgcggag attaatcctc aacgtaaagc ggaaatactt cgacgtggat
     6541 atgagtttgg agaaagtcgg gtcatctgcg gtgcgcattg gcaaagcgat gtagaggctg
     6601 ggcgtttaat gggagcatcg gttgttgcag tacttcataa tacacctgaa tttaccaaaa
     6661 gccttagcga agccaaaaaa gagtttgaag aattaaatac tcctaccaat gaactgaccc
     6721 cataaagctg gacagcctgt atcaggctat ggagggccca tagacaaatc taccctatat
     6781 gagcatagga ggagtctatg ggcacaccac gttttacccc tgaatttaag ggattactgg
     6841 aaaggctggg acatatccct ccggcagaag cagaaaaagc ttattatgct gccatcggaa
     6901 acgatgatct ggcaacctga gttcacagat aaaacattct ctaggaaact cggggcggtt
     6961 ccgttcacca catgcaatgt ggtgttgcag gggaacggtc tgcaccatcc cctatgtcga
     7021 tcaatataac agaaatgaca acttcagatt cagggcacaa cctaaatata ttttaggtca
     7081 cctctcaaat cgtttgcctg ataccgctcc attttttaat aaaaaaatca atcatttttg
     7141 aatctttgct tttcattgca ttgtcaagca tagtgtcccc actatttgct ttttgaaatt
     7201 ttttgtttac atcaaccaat ccattattga taaaatattc tagcacttta tagttggcac
     7261 catctttact aagtaagtat tcaacatcat acagattaca atatctattg tttttcattt
     7321 tctccatctc tgcgacatct tgttttgtaa aattaaattt agatagtaaa taaagtgcta
     7381 tatcagcttt tttattgcct aaagaataag caagggcctg atgagccatt tttttatcag
     7441 tcactttatt gttaatctct tcataattag atgctttaat cgcatcctcc aaagatatgt
     7501 ctctgagttt tatttttttg aagttatctg tacttagcat tcttggtata tgatactcaa
     7561 gctgaaacac aaagtttata attctatcca gactaacagg atcaatattt ttgttataac
     7621 aaaactctct gaatccttga tctttactct ttcttataaa atcaataaga tgcaacccaa
     7681 ttccaagcct catatcttta gtgttaaata ttggaacatc agttttcccc tgattaccat
     7741 gcacatacct gctaacttca tccacaacct ctttaaagtt atctaagaaa aatgattggt
     7801 gctcatggta aaaaacaggt ggaatagcat tatcaaagtg atcggttaat gtcatatatc
     7861 catatggaga gtcatgatca atgatatacg cattggcgcc aaaatctaca gtatgatgtg
     7921 ttttatttag tgggagtttt tcttgatgat tggaaatttc gaccccaaaa aaaacaaaat
     7981 cgtggttaga gatttcttta atatcctcag agtatgtatt cttatgtgtt tgtatatttt
     8041 cagactgaat tttatca
//
LOCUS       DAJTQQ010000151         7919 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000151 DAJTQQ010000000
VERSION     DAJTQQ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7919
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.151"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            1..1238
                     /gene="yjeM"
                     /locus_tag="OO309_003788"
                     /pseudo
     CDS             1..1238
                     /gene="yjeM"
                     /locus_tag="OO309_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1389..4700)
                     /gene="mscM"
                     /locus_tag="OO309_003789"
     CDS             complement(1389..4700)
                     /gene="mscM"
                     /locus_tag="OO309_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR5848755.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4722..5690)
                     /gene="psd"
                     /locus_tag="OO309_003790"
     CDS             complement(4722..5690)
                     /gene="psd"
                     /locus_tag="OO309_003790"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR5848756.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5787..6839)
                     /gene="rsgA"
                     /locus_tag="OO309_003791"
     CDS             complement(5787..6839)
                     /gene="rsgA"
                     /locus_tag="OO309_003791"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR5848757.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            6934..7479
                     /gene="orn"
                     /locus_tag="OO309_003792"
     CDS             6934..7479
                     /gene="orn"
                     /locus_tag="OO309_003792"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR5848758.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7690..7765
                     /locus_tag="OO309_003793"
     tRNA            7690..7765
                     /locus_tag="OO309_003793"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7723..7725,aa:Gly,seq:gcc)
BASE COUNT         1805 a         2054 c         2145 g         1915 t
ORIGIN      
        1 attggtacgt ttatgtggtt ttcctcttat atcatctgga tggtgagtac ctccgcgaaa
       61 gtttgggtac cgttctcaac attcctctat ggtagtgaca tgacccagca ctggcgtatt
      121 gccggactgg agcctacgca ggtggttggt ctgctggcag tagcatggat gattctggtc
      181 acagtcgttg cttcaaaggg gattaataaa attgcccgca ttactgcggt gggcggtatt
      241 gcagtaatgt gtctgaattt agtattgctg ttagtaagca ttactatttt gttattaaat
      301 ggtgggcatt tcgcgcagga tattaatttc cttgcatcac cgaacccggg ttatcagtcc
      361 ggtctggcaa tgctatcgtt tgtggtattt gctatttttg cctatggcgg aattgaagcg
      421 gttggtggtc tggtcgataa aacggaaaat ccagaaaaga actttgccaa aggtattgtt
      481 tttgccgcta ttgttatttc aatcggttat tcgctggcaa tatttttatg gggcgtcagc
      541 acaaactggc agcaggtatt aagtaatggt tccgttaacc ttggcaatat tacctatgtg
      601 ctgatgaaga gcctcggggt gatgctgggt aacgcactgc atttgtcacc tgaagcgtca
      661 ttgtcgctgg gcgtatggtt tgcgcgtatt accggacttt cgatgttcct cgcttatacc
      721 ggtgcgtttg ctattcaccg ctgaaagcca tcatccaggg gacgccgaaa gcgttgtggc
      781 cggaaccgat gacgcgcctg aatgcgatgg ggatgccgtc tatcgccatg tggatgcagt
      841 gcgggttggt tactgtcttc attctgctgg tttcgtttgg tggcggtacc gcatcggcgt
      901 tctttaacaa gctgacgctg atggcgaacg tgtctatgac gcttccttac ctgttcctcg
      961 cgctggcttt cccgtttttt aaagcacgtc aggatctcga cagaccattt gtgattttca
     1021 aaacgcgtat gtcggcaatg attgcgacgg tggttgtcgt tctggtggtg acatttgcga
     1081 acgtcttcac cattattcag cctgtggttg aagccggaga ctgggacagc acattgtgga
     1141 tgattggcgg ccctgtcttc ttctcgctgt tagcgatggc gatttaccag aactattgca
     1201 gtcgcatggc gaataaacct gagttagctc tcgactgatc atatgaaatc ctgcaactca
     1261 tcgtctctcc ggcgatgagt tgtcaggaag taatgcgtat ggatgatact ctccagggcc
     1321 ggtgataact gaagatgatg ttaagctgct aaatgagcca cgttgacatc atttggcagc
     1381 ttcatgcatt acaaacttcc cgcctgacga cctttgcccg cagacgtcag cgttctcccc
     1441 gtttgtttac cgttgaggct ttccagacgc atctggaagg gcgggaatgg catatcgata
     1501 ccgtgggcgt ggaagcctgc cagaatcagc tggtggatct catggcgtag cggcatacgg
     1561 tgacccatct cagcggcgta aatacgcagc tcgaaaatct gaatcccctg ttgcagatcc
     1621 accaggaaga cttccggtgc cgggttgtcg atcaccagcg agcagcgacg cgctgcggtg
     1681 agcaggattt ccgtcacttc ttcactgttg gcatcggcag gggccggaat cgtcaacact
     1741 acgcgcgtga ccgagtcaga gagcgaccag ttgataaact gctcggtaat aaacgccttg
     1801 ttcggcacga ttatctcttt acggtcccag tcgctgatgg tggtggcgcg ggtgttaatt
     1861 tttgtcacgc taccagtaag atcgcggatc gttaccgtat cgccaatgcg aatcggtttt
     1921 tcgaacagga tgatcaggcc agagataaag ttggcgaaaa tttcctgcaa accaaaaccg
     1981 agaccaacac cgagcgcggc aaccagccac tgcaatttcg accactcaat accaatcatt
     2041 gagaagccga ccagcccgcc aatcagcatc agcagatatt ttgtgatggt ggtgatggcg
     2101 taacccgtac ccggcgttaa atccaggtgc tgcaaaatcg ccagttccag cagcgcgggc
     2161 aggttgcgca ccagctgcgt ggtgatgata aataccagaa tggcaatcag caccgcaccg
     2221 agggtaattg gctccagact ttctacgccc tgtaccgtgg aggtgacatc ccacagcgaa
     2281 atattttcga ggaagccgaa agcggaatgg atttctgacc acagcacaat gaccgaaagc
     2341 agggcgatca acatcaaaat tgagcgcacc agccgcaagg attgcgcact gatggcatcg
     2401 agatcgactt cgctttcatc gacttcaatt gctccttccg ggctactgtg atgatgcgct
     2461 tcctcttcgc cacgcgcacg ttgcgctaac atctctgccc ggcgatgctt cgcccggtca
     2521 aacgccagcc tgcgacgctg gatcagcatc cagcggcgga taacgtgata aaccaccagt
     2581 agcaggaacc agatggcaac cgaggtttca agcctcgcta acagtgcctg cgccgttgcc
     2641 agataaccca ccgccgacgc cagaatggca accagtggtg cgccaatcat catgttccac
     2701 agcatatggt tggtaatgtt gtcgccgctg ccttctttgt tgagatacaa cggaataccg
     2761 gcttttttca ggcttagggt gaccaccgcc agcgcaccgc aaatgaggat aaagcaaagc
     2821 cgtcccagcg aaccggaaaa ttcgcggtcg tcgaggttat cgaacatcat cagcgccata
     2881 atcagcccga tgctcatcag gtagtagcgc atcccacggg aaacacgttc gcgcggccag
     2941 ccaaaatgag cgataaacaa gccgttcggg cgggcaaagg tggcgcaaat catcactacc
     3001 cacagcagcg gcacggtggc cgttacacca tcgccaatcg cgaccgccag cggataaggc
     3061 cacgcctcgc gcaagccgta acccagcgtc atccacagca ccggtaacgg tgacgcgacg
     3121 agaatcgacc agaaaagagt gcgcaacgtc agccagaagt gatcctgagt cactttgccg
     3181 actttcgccg ccgaacgttc aagaaaacgg gtgaaatagc ggcgcgagta aatactgcaa
     3241 ccgaccagaa tcaacgcgcc aaacagcggc aaaattgtct ctttgctggt cagcatcatc
     3301 acactggctt tgcccaactg actgaaggtg tccagcgaaa tgagacgacg cagatcctgg
     3361 gcgatttcca gcggccaggc gatggtcatc gggcgcacgt cagaggccca gaacagatag
     3421 cggtgcgttg cttcgttcac ctctttcagc gcatcctcca gttgcctgtt ggagactttc
     3481 agcttggtca gttccaatag tagcgtgtcg ccaccctgca acaatgagtt cagcaactca
     3541 cgctgagtgc gcagttgtgc ttccagaata cggttttgct cggcggtcag cggctgaccg
     3601 tcggcctgat ggatttgccg tagcagcggc tgtttattaa gtaaatcctc ataacgtaac
     3661 cgttgcacac gcaactgcgc catttcggta tcaagctgtt gtggttttgg catttctggt
     3721 agccgtgcca cctgcgcccg cagcgcttcg ccgagcaggt tggacgatcc cagccattgc
     3781 gactgttcac gcagcgtatt caacgcctgc cggacctgta acgtctggct ggcagcctga
     3841 cgctgttgcg aggcaacgag ctccatccgc tgcgcctgtt gattcagcgc tgccgatagt
     3901 tcgcggttaa ttttgaattg cgcgacgata tctttcggca aatcggcact gttttctgcc
     3961 agtaattcgg tactttccag cgcccgctcc gcctcaagct gacgttggct attcaactga
     4021 ttacgtaagg cctgcaaata cgcatccagt tgctggctct ctttttccgc cagctctgag
     4081 cgtaagcgcg ctaattcctg gcggttattg gcagacagct gcgccagctc cagttcatca
     4141 acgagcgcct taagacgtgc agagtcagac tgcaacgcga aattttgtgc ctgattgagc
     4201 ggagtattgc cggtaagcgt tcccaggcgg cgctcgatct catttaactg acggcgggcg
     4261 tcggtttgct gttgcggcag ttgattcagc gaatcggcaa tctcgcgggc gcgctcctgc
     4321 tcttgctggg cctgacggct tttatccagc aactggctgc tgacctggag aatttcctga
     4381 ttcagcgcgt cggtagacat tcccggcgac acgctgcgcg gctcgtcacg aatgttgttt
     4441 aattgtgcgc gcagagtagc ggagagtttc ggataattat cgataacttg ctgatattgt
     4501 ttgatgcgct caagggaacc ttttcgttcc tcaagcgcat ttaaggcaga ctggagcgcc
     4561 tctacgactt ccggctgtgc gggtttcgcc gcttttgcct gctccagttc ctgagtgatt
     4621 tgtttgctat cgggggccgt cgcggcgtac gccccccaac tgaggcacca ggccatcaga
     4681 aaagtgataa tcaggcgcac gtcagcgttt cctttgatgg attagacctg gtctttttta
     4741 tcgtcaacca atgggctggc gtcgtgttct gcttcgatct cttcagcagg aagtggggcg
     4801 ggttcagcgt ctggcgtaac aaaggtttcg gtagatactg ccagcggctg gccaattttc
     4861 gtgacagaca ggctttccag ttgctcaacc agattcactt tacccggtgc aaacaggttg
     4921 ataacggtgg aaccgagttt aaagcgaccc atttcctggc ctttcagcag tgccacagaa
     4981 ccgtcgtttt ccccggcagg ccaggtccag cgcttgatga taccttcgcg cggcggcgta
     5041 atggtgcccg cccagaccgt ctcaatgctg ccaacaatcg tcgctccgac cagaatctgc
     5101 gccattgggc caaattcggt atcgaaaagg caaatcacgc gttcgttacg ggcaaacaga
     5161 tttggcacgt tctgagcggt gagatggtta acggaaaaga gatcgcccgg cacgtagatc
     5221 atctcacgca gaataccgtt gcacggcatg tgtacgcgat ggtagtcacg cggggagagg
     5281 taagtggtca caaacgtacc gttgcggaac aggtccgcca tcagatagtt gcctgccagc
     5341 agggcttcga ggctgtagtt gtggcctttg gcttgcagga ttttatcttc ttcgatttta
     5401 cccaactggc tgataacgcc atcggcaggc atgaccagta cattcggatc ggtatcgatt
     5461 gggcgtactt cgtcacgcag cggacggaca aagaattcgt taaaggtgcg gtagctggcg
     5521 gtgtccggct tttgcgcctc tttcatgtcg accttgtagt atttaacgaa cagatcgata
     5581 accagttttg tcagccatcc tgcccgcttg cttgcgcccc aacccgccag gcgagtaagc
     5641 catagtttcg gcagaatgta ctgtagcgaa agtttaaatg aatttaacaa ggtagcctcc
     5701 aggccattgt tttgtcgttc ctgatccggc caacatgccg gatcctgaaa aaaaggggac
     5761 gattctaacg acggttagct taattgtcag tcatccgtat cagaaaagtt tttacgcgtt
     5821 tttacctgcg ccatgctttc cagaatacgg tgatagtttt cgaaacgggt ttccgcgatt
     5881 ttcccttcct caaccgcttc ccggatagcg cagcccggat cggtatcgtg tttgcaatcg
     5941 cgatatttac acagacctaa atagtcatgg aattcgacaa agccctgagt gatttgttcc
     6001 ggctccaggt gccagaggcc gaactcacgc acaccagggg aatcaatcac atcaccgccg
     6061 tgcgggaagt gatacagccg agcggcggtc gtggtgtgct gaccgagccc cgagttgtca
     6121 gagacatcgt tggtcaggat ctctttttgc agccccagca acgcgttcag caggctggat
     6181 ttaccgacgc cagactgccc ggcaaaaatg ctgatgcgcc cggtcaacgc ctcttccagc
     6241 ggtttcagcc catcctgagt atggctggaa accatcaata cgcgataacc gatgttgcgg
     6301 tagatatcca tctgctcgtt gacgaacgcc atgccttcgt cgtccagcag gtctatcttg
     6361 ttgagcacga taatcggctc aatctgcaag gtttcgcagg ccaccaggta acggtcgata
     6421 atattgagcg acagctccgg caaaatggcg gagacaatga caatctggtc gatgttggcg
     6481 gctatgggtt tcacgccgtc gtagaaatcc ggacgcgtta acaccgaggt gcgctcatgc
     6541 accgcttcca cgatcccttt gacatttacg ccttccgccg ccggtttacc cggacgccag
     6601 actacgcggt cgccggttac cagcgaacgg atggtacggc gaatattgca gcggtgaacg
     6661 tcgccatcgg cggattccac atcagcgtgc ataccaaagc ggctgatgac gataccttca
     6721 tcaggctcgc caaacagatt gtcgtcgtag tcgggcttct ccttagacgt tttaagacga
     6781 cgctggtgat tggcgttcac gcggcgctgc tggcctttgg agagtttatt tttactcaat
     6841 cgtactgact cctggtcgcc cctgatgggc aaaacatcta tgatacacgc aattgtggat
     6901 caatatagtc actgtgaatg ggtggaaaat agcatgagtg ccaatgaaaa caacctgatt
     6961 tggatcgatc ttgagatgac cggtctggat cccgagcgcg atcgcattat tgagattgcc
     7021 acgctggtga ccgatgccaa tctgaatatt ctggcagaag ggccgaccat tgcagtacat
     7081 cagtccgatg aacagctggc gctgatggat gactggaacg tgcgcaccca taccgccagc
     7141 gggctggtag agcgcgtgaa agcgagcacg atgggcgatc gggaagctga actggcaacg
     7201 ctcgaattct taaaacagtg ggtgcctgcg ggaaaatcgc cgatttgcgg taacagcatc
     7261 ggacaggacc gtcgtttcct gtttaaatac atgccggagc tggaagtcta cttccactac
     7321 cgttatctcg atgtcagcac cctgaaagag ctggcgcgcc gctggaagcc ggaaattctg
     7381 gatggtttta ccaagcaggg gacgcatcag gcgatggatg atatccgtga atcggtggcg
     7441 gagctggctt actaccgcga gcattttatc aagctgtaaa atttttcctg gtcacgtaag
     7501 cgccggaatc cacttgcggc ggcgtgattt tgacgctaaa tcgaacgtct tgctgataat
     7561 ttcagcactt gagataaaaa cgcaaaaaaa acttttttgg ggggttgcag agggaaagat
     7621 ttctcgtata atgcgcctcc cgtaacgacg cagaaatgcg aaaattacga aagcaaaatt
     7681 aagtagtacg cgggaatagc tcagttggta gagcacgacc ttgccaaggt cggggtcgcg
     7741 agttcgagtc tcgtttcccg ctccaaaatt tgaaaagtgc tgcaaagcac agaccaccca
     7801 atcgcagtat ttcgaattgc gacaaggcgg caactgaacg aatccttggg aacttacaaa
     7861 agtatgtgac taaggtgagt gaaggcagcc aaggcagtag cggttcgaaa gacgaagat
//
LOCUS       DAJTQQ010000152         7750 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000152 DAJTQQ010000000
VERSION     DAJTQQ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.152"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            1..299
                     /locus_tag="OO309_003794"
                     /pseudo
     CDS             1..299
                     /locus_tag="OO309_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612101.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(329..3241)
                     /locus_tag="OO309_003795"
     CDS             complement(329..3241)
                     /locus_tag="OO309_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848759.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3244..3360
                     /locus_tag="OO309_003796"
     CDS             3244..3360
                     /locus_tag="OO309_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5848760.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3724..4566
                     /locus_tag="OO309_003797"
     CDS             3724..4566
                     /locus_tag="OO309_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5848761.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4569..5657
                     /gene="capU"
                     /locus_tag="OO309_003798"
     CDS             4569..5657
                     /gene="capU"
                     /locus_tag="OO309_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5848762.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5662..6612
                     /gene="virK"
                     /locus_tag="OO309_003799"
     CDS             5662..6612
                     /gene="virK"
                     /locus_tag="OO309_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR5848763.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6677..7621
                     /gene="lpxM"
                     /locus_tag="OO309_003800"
                     /gene_synonym="msbB"
     CDS             6677..7621
                     /gene="lpxM"
                     /locus_tag="OO309_003800"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5848764.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2085 a         1730 c         1829 g         2106 t
ORIGIN      
        1 ccctcgtcag cagttccgtc agcactgcga cagcgttctt tacccggtca aaacagcgtt
       61 gcggtgcacc acgcctgacg gatgaactgc gtgctcaggg ttaccacttt aacgtaaaaa
      121 ccgtggcggc aagcctgcgc cgtcaggggc tgagggcaaa ggcctcccgg aagttcagcc
      181 cggtcagtta ccgcgcacat ggcctgcccg tgtcagaaaa tctgctggag caggattttt
      241 acgccagtgg tccgaaccag aagtgggcgg gagacatcac gtacttacgt acagatgaag
      301 ttcggttaca tcctgtcagc acggaacctc acgctttttg agcggcacga cggatccccc
      361 ccacccagta tccgacagac ttttcactgc gttcgtaaaa acccttcttc cctcccagca
      421 tatccactaa ttccggtgac atatcctgca gataagtctg caaatcatgc cccagatctt
      481 ccggtgactg caactcataa accgcgcgcc acacatcacc cttccttatt ttaagccccg
      541 ggttaagaat atactgtccg tggttcagcc tcagaaaaag cttttttcca taaggatttt
      601 tgctgttaac ttcatgttga gagagctggc tgctgatata actccttcgt ttgcggcgtt
      661 ccggcagcaa cgcttcagga agatgaacca cacactctgc cagtcgtgcc gcactgaagg
      721 cttccgaaaa aaacagagca cttcccagat gcgtgtggaa cagcgcctgc atcaggatca
      781 ccagaaacat tgccatctgc cggttgtcca gtttgatgag gcgtccttcc acctgcagtg
      841 acagactttc aggtttcagc agggtataca actgcgcact tttctgttgc gcataacggg
      901 gcgtactgac cgccgctgac agaacttgat gataagccgc cagcccgtca ttaccggtca
      961 gcaagggatc tgcaccacgc tccagcagta actgaaccag tgcaatatta cctgcccggg
     1021 ctgctgacat cagcggagta agattaaacg gcgaacggtg atcaacacca taacgttcaa
     1081 tatcccgcaa cacggcaacc gggttattca ttgagtatgg ccggaaatac ttcagctcca
     1141 gtaactgcag agtcttcgtt ctgggctgcc ttgtgggctt aaaatcttcc cggtacagtg
     1201 cattatacag cgggaacagg gaataaatca gactgtactc cagcgcctgc agttgtatgg
     1261 ttttatcggc tgtacctgcg aggatatgct gtttccactg tcgtgcatcc tctgcggtga
     1321 taatctgcca tggcatctca gatgtctgca ggatccggct acggatggtg tccgcctgct
     1381 cctgcttccc ctgtttttca agcagatgtg cggttttctg ccactcatca cggctggaaa
     1441 tctcttcctg cagattcaga ggctcttcct gatgcgtgag cgcaagcaga ctccacagag
     1501 gatgctccac ctgctgttca acaagataca cattgtgttt agcacgggtg agcgcaacat
     1561 acagcgcgtt ggtaaaaaat ttatagattt ccgctgaacg atcctgcctg tcccgcggac
     1621 gggaataacg ggcctcgccc tcaagatcag ccggtgtaag accctcgcaa atatcatcaa
     1681 atgcctgccg ggcagcgctt actatattgt aaaggatcac cgtttcgtat tccagcccct
     1741 taacctcctg aaccgaaaag accagcggcg tactgaacca gcatcgcgcc tgtgctttct
     1801 gttccggatg catcacaatg accgctacac gattcgaaag tgaggttttg gcattcagtt
     1861 cctgctttgt ctcttcccta tcgtccagca accggatggt cccttctgcc tgaccgcagc
     1921 ttctgacaaa atggtgactt tcccgatcaa tagcactaaa gcggacctgt ttgagtctca
     1981 gcaaccggtt agccagtgca gtgacgtggt gcccgttacg gtaattcgcc tgcaggatat
     2041 taacggttgt gtcgtttccc tgttgctgcc ggaaaaagag acttttgagc gaactccatg
     2101 agaaaaagct cggatgaact atctgattgg catcaccaca gagtaaaaac tgaccgggat
     2161 gacgtagcgt tttcagtacc agttgcagtt gaacaggcgt catatcctga acttcatcaa
     2221 caaaaatgac atcataacgg ggagttgcca acgaaagata ctgatgactg agcagattac
     2281 tgtcatactg atgcgactgt ttcagccatg cgatataacg ttcaaacagg acatagaccg
     2341 tcgggcggtc ctccatgcca tagagcgact gccgtatccc cagtgacaga tacgcctccc
     2401 gggacagcgg cccattcccg gaggcaaccg caccgatcac gcccctgaac tcctcataca
     2461 gggtatgggc agcccccagt gcggcgatag ccctgttgcg gggcagccag tcgctgaacg
     2521 cactgaaagt cacctccctg ccttccggaa tgcgcagagt ttccagaaat tccgtcagcg
     2581 aaagaaagtc aatattctgt acctcactcc cttcccctga agcgtcatag agagtacgcg
     2641 ctttttccac cagaaatgag gaaaggctga gatacagtat atcaccagca gcctgcttca
     2701 ttttttccag taccagcgcc gtcttaccac ttcccgccga gccaacaatc accagcggta
     2761 gcgggtgttc gtacagtgcc tgctgatcat catcaaagga cagcatttta tccaggcggt
     2821 ggaaacgccc atgagacgga ttgatatacg tcagcgcttc agtcgcgata tcaacaggat
     2881 ccggcactgg ttgttgttgt aagcgccctt catcaatcac cacgtttctg cgaagaaaac
     2941 gtgaagtgtt ataggcatga ttgcgaatat attccagaac aaggcagata gtttcaccgt
     3001 ggtagcgata cagcgaaaac agtaatcggt cccggatact gagccgggcg cggtaaagat
     3061 tggtgtcaat tttacgaaca tcagcctgaa caaagttatc ggtggcaata gcctgtgcaa
     3121 attttttata acccgggatc gtgtcaggat tcagagtatg ataaagacag attttcatgg
     3181 ctgccggttt ccgtcgtgca aaaaacatgc tgattttacg aaggaatgcc ttactgaaca
     3241 accttgcccg gaataccccc cgaaatcaga tggaacaatc agaaaaatgc tctgcagtta
     3301 tggaataaag aaggtggggt ggtacgccgg gagggcgatc cgctggaaat ggcgcagtag
     3361 ctgcaggcct gtcatgatgc aggaatagaa gttcgtgtac aggttaatga aagcgtcacc
     3421 ccgtaaataa actctgcgct gaaattcctt tttttcattg gagtacctgt gttgttctga
     3481 ggtgaacata tcacctctgt tcaggtagcc aaattcagtg tgccacctca aggttcttag
     3541 tgagtcgttt ttaaaagata tgataatata tcattgttta tgaattgttg atgtttaatt
     3601 tagtaaatta tatcaaatat gccatgggtt tgctatgtta ttcatccagt atctggaaat
     3661 aaaatgcacc agctttattg ggttttcctt ttgtctctgc ttgctaggtg ccagaaactt
     3721 taaatgttaa acgaaggggg cattttgttc aaagccaatc atgttccggt attaatgtat
     3781 catcatgtca gtcattgccc cggactggtt acactttctc ccgtgacatt ccggaagcaa
     3841 ataaaatggt tggctgaaaa caactggaaa acactgtcat cagatgaact ggagtttttt
     3901 tatcggggcg gaaaactccc ccggaaaagc gttatgttga catttgatga tggatacctt
     3961 gacaactggt ttcaggtata tcctttatta aaagagttta atctcaaagc acatattttt
     4021 ttaattaccg gttttattgg gaatggtccg gttcgtcact ctcctggaaa ggaatattct
     4081 catcgcgatt gcgaacatca aatagcgaca gggaatgctg ataatgttat gcttcgctgg
     4141 tctgaagtaa acgaaatgtt gcaatctggt ctggtcgaat ttcatgttca tacgcatact
     4201 catacacgct gggataaaaa gttcagttca cgagaagaac agtgtaaaca tcttcggcag
     4261 gacttgttga gcgggaggga atatttgaaa gaaatgacag gaaaatgcag taaacatttg
     4321 tgttggccgg aaggttatta taacaaggat tatattcagg tcgctgaaga acttggattc
     4381 tattatttat atacaacaga aagaagaatg aatgctcctg ctaaaggaac tacacggata
     4441 ggaagaataa gcaccaaaga acgggaaagt tgcgcatggc tgaaaagacg acttttttat
     4501 tatacaaccc cgtttttttc atcgttgctg gcctttcata aggggccaag attaccggat
     4561 gattaataat gaatatacta tttacggaat catcaccgaa tataggtggt caggaattac
     4621 aggctgttgc tcaaatgaag gccctgaaga aaatggggca ttcagttctg cttgtctgca
     4681 gggaaaacag caaaattgct tttgaagcca gtaaattggg gattgatatc acattcgcgt
     4741 tatttcgaaa cagtcttcac atccctactg catggagatt actcggaata gttcatggtt
     4801 ttcagcccaa tgcaatcgtt tgtcacagtg ggcatgatag caatattgtt ggtttagtac
     4861 ggttattcac taggaaacat ccattcagaa ttatcaggca aaagacatat ttgacacgaa
     4921 aaacaaaagt tttttcaata aatcattttt gcgatgaggt gattgttccc ggaacaagta
     4981 tgaagacaca tctggagcag gaaggatgtc gaacccgggt tactgttgtg cctccaggct
     5041 ttgatttcca gaaattatac gttgattccc gaaacagttt gcctccaaat gttctttctt
     5101 ggctggcgtc ccgaagggga tgccccgtta ttgctcaggt aggaatgttg cgcccggaaa
     5161 aagggcacga atttatgttg aatttactgt tccatttaaa aatgaatgga cgacagttct
     5221 gttggttgat tgtggggtct ggttcgcctg aactgcggga gcatttacag tatcagattg
     5281 acagtatggg catgcatgat gatgttttta ttgctgacaa tgtttttcct gccgcccccg
     5341 tatatcgggt tgccagtctg gtggttctgc cttcagaaaa cgaatcgttt ggtatggtac
     5401 tggcagaagc atcggcattt tctgtgcctg tactggccag tcagattggt ggaatccctg
     5461 atgttattca gaataaccag accgggacat tgttaccagc aggtaataag cacgcatgga
     5521 tgtgcgccct gaatgatttt tttaatgacc ctgggcgttt ttatcagatg gctcgccagg
     5581 caaaacagga tatagaagag cggtttgata ttaataaaac tgcgttaaaa atactcacat
     5641 tagcgaagca caagtaacaa tatgttttct gtaagtaact tatcatttat cggtttcctt
     5701 aaaaggattg ttttttcctc agattcactc ccggggaagt gggaacacag aaaattccgg
     5761 ttcatgtaca ttttgcgatg tgctataaat ccggttgcca gtattcgata ttattacgaa
     5821 ctgcgttcct tgcagtgcat tgaggatatt ctggcaatac aacccacgtt gccagcaaga
     5881 attcatcgac cttatttgca taaagggggg cgagcctggt cccgggggca atatattctt
     5941 gagcattatc gctttgtgca gaatttgcca gaaaaatatt ctgaattttt gtttccacaa
     6001 aaatcggtat cattagtcca attcatcggg aaagatggag aggattttga tatccagtgt
     6061 tctcccagtg gttttgaccg ggaaggagag ttgatgttgt cattgttttt caataaaata
     6121 gtgattgccc ggttaacatt ttcagtcatt ctgactcaaa atggacacac cgcttttatt
     6181 ggcggattac agggagcccc caaaaatacc ggacctgata ttattcggtg cgcaacccgg
     6241 gcctgttatg ggcttttccc aaaacggatt atttttgaag cattttgtgc gttgatgaaa
     6301 gcttgtaatg tctctgagtg tctggcagtc agtgagcaca gtcatgtttt ccggcaattg
     6361 agatactggt atcagaaacg caaaaccttt gttgctgtct acagtgattt ctgggagtct
     6421 gtagcaggga aaacctgtgg tgactggtac aaattaccaa cacaggtggt tcgaaagccg
     6481 ctaagcaata tagctagtaa aaaacgctct gagtatcgaa aacgatacgc tttgctggat
     6541 tatatccatg aaactgctat ccgctctttg gatgcatatc ctgtaaactc agaacatcag
     6601 gacttaaatt aaggtttgat gtattctctg aataaaatat taatgatgat tttggtaggg
     6661 gcattcgcac taaataatga aaaaatacaa atccgagttt attcctgaat ttaagaaaaa
     6721 ttatctttcc cctgtttact ggtttacatg gttcgttttg ggaatgattg caggtatttc
     6781 aatgtttccc ccttcattca gagatcctgt cttggccaaa atagggcgtt gggtgggcag
     6841 attgagcaga aaagctcgtc gcagggcgac gattaattta tcgctttgtt tcccggaaaa
     6901 gagtgataca gaacgggaaa taattgtcga caatatgttt gccacagcat tgcaatccat
     6961 agtgatgatg gcagaactgg caattcgtgg tccggaaaag ttccagaaac gagtgttctg
     7021 gaaaggcctc gaaattcttg aggaaattcg gcacaataac agaaatgtga tttttctggt
     7081 tccccatggc tggagcgtgg atattcctgc aatgttgctg gcagctcagg gggaaaaaat
     7141 ggccgccatg tttcatcagc aacgaaatcc agtgattgat tatgtctgga attctgtacg
     7201 gcgtaaattc ggggggcgct tacattcccg ggaggatggg ataaaaccat ttattcagtc
     7261 agtacgccag ggatactggg ggtattacct tccagatcag gatcatggtc ctgaatacag
     7321 tgaatttgct gatttttttg cgacctataa agcgacatta ccaattattg gacgtctgat
     7381 gaacatcagt caggctatga ttataccgct tttcccggtt tatgatgaaa aaaaacattt
     7441 cctgactatt gaagttcggc caccaatgga tgcatgcatt gccagcgcgg acaataaaat
     7501 gattgcccga caaatgaaca aaacagtgga aattttggtg gggtcacatc cggaacagta
     7561 tatctgggtt ttaaaattgt taaaaacgcg caaatcaaac gaagcggacc cgtacccttg
     7621 aaattataaa gtacaggtat ttccactagt tgtttcttac aggttaccaa tcgaaacaca
     7681 tcccccttcc gtgtgttgcc cgtttggcac ggaggactgt gtagatgcag ttcgttaaat
     7741 ttaatttatg
//
LOCUS       DAJTQQ010000153         7742 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000153 DAJTQQ010000000
VERSION     DAJTQQ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7742
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.153"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..1011
                     /gene="ydfJ"
                     /locus_tag="OO309_003801"
     CDS             <1..1011
                     /gene="ydfJ"
                     /locus_tag="OO309_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCR5848765.1"
                     /translation="LLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGT
                     NCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKV
                     NDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIP
                     TDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPST
                     IMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGI
                     FCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSR
                     SL"
     gene            1100..2560
                     /locus_tag="OO309_003802"
     CDS             1100..2560
                     /locus_tag="OO309_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5848766.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2596..2799)
                     /gene="ydfZ"
                     /locus_tag="OO309_003803"
     CDS             complement(2596..2799)
                     /gene="ydfZ"
                     /locus_tag="OO309_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR5848767.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2977..3663)
                     /gene="ydfH"
                     /locus_tag="OO309_003804"
     CDS             complement(2977..3663)
                     /gene="ydfH"
                     /locus_tag="OO309_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR5848768.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3752..4498)
                     /gene="ydfG"
                     /locus_tag="OO309_003805"
     CDS             complement(3752..4498)
                     /gene="ydfG"
                     /locus_tag="OO309_003805"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR5848769.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4635..6680
                     /gene="dcp"
                     /locus_tag="OO309_003806"
     CDS             4635..6680
                     /gene="dcp"
                     /locus_tag="OO309_003806"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR5848770.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6725..7243)
                     /locus_tag="OO309_003807"
     CDS             complement(6725..7243)
                     /locus_tag="OO309_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR5848771.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7519..>7742
                     /gene="ydeI"
                     /locus_tag="OO309_003808"
     CDS             7519..>7742
                     /gene="ydeI"
                     /locus_tag="OO309_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCR5848772.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKG"
BASE COUNT         1940 a         1811 c         1957 g         2034 t
ORIGIN      
        1 ttgctggtga cgctgcgtat tattcaggga ttgggtgcag gtgcggaaat ttccggtgcc
       61 ggtacgatgc tggcggaata tgcgccaaaa ggtaagcgcg gaattatctc ctcatttgtg
      121 gctatgggaa ctaactgcgg caccttaagc gcaacggcaa tctgggcctt tatgttcttc
      181 attctcagta aagaggaact gctggcgtgg ggatggcgta taccgttcct ggcgagcgtt
      241 gtcgtgatgg tctttgctat ctggttgcgt atgaatctga aagaaagccc ggtctttgag
      301 aaggttaacg acagcaacca accgacagca aaacctgcac ctgctggtag catgttccag
      361 agcaaatcct tctggctggc aacagggctg cgttttggtc aggcgggtaa ctccggttta
      421 attcagactt tccttgcagg ctatttagta cagacgttat tgtttaacaa agcaattcca
      481 acagatgcat tgatgatcag ttcgattctc ggctttatga ccattccgtt ccttggttgg
      541 ttatccgata aaattggtcg ccggatcccg tatattatta tgaatacctc tgcgattgtg
      601 ctggcgtggc caatgctttc tatcatcgta gataaaagct atgccccgag caccattatg
      661 gttgcactga ttgtgattca taactgtgcg gtgctgggat tatttgctct ggaaaacatt
      721 accatggcag aaatgttcgg ctgtaaaaac cgcttcaccc ggatggctat ttcaaaagaa
      781 attggtggtc ttatcgcttc cggttttggt cctatcctgg cgggtatttt ctgcaccatg
      841 acggaatcct ggtatccgat cgccattatg atcatggcat attcagtgat tggtttaatc
      901 tctgcgctga aaatgccaga ggtgaaagac cgtgatttaa gtgcgctgga agacgctgcg
      961 gaagatcaac cgcgtgttgt aagagctgcg caaccttcca gaagtctgta aaccctcaat
     1021 cccttctctt accggagagg ggatttttat tcatataaca aaacatatag cttgccatat
     1081 ttaaatttaa ggaattgtta tgggaaataa tttgttatca gcaaaagcga cactccctgt
     1141 ttatgatcgt aataacctgg ccccaagaat tgttcattta ggctttggtg catttcaccg
     1201 tgcgcatcag ggcgtgtatg ccgatattct tgctacagaa catttcagtg actggggata
     1261 ttatgaggtc aacttaatcg gcggcgaaca gcaaattgcc gatttacaac agcaagataa
     1321 tctttatacc gttgcggaaa tgtcggccga tgcgtggacg gctcgcgtcg ttggcgtcgt
     1381 taaaaaagcc ttgcacgtac agatggatgg cttagaaacc gtgttggctg cgatgtgtga
     1441 accgcaaatc gcgattgtct ctctgacaat caccgaaaaa gggtatttcc actctccggc
     1501 gaccggacag ttaatgctcg agcacccgat ggtcgctgcc gacgtgcaaa atccccacca
     1561 gccgaaaaca gcaacagggg tgattgttga ggcgctggct cgccgtaaag cggcaggact
     1621 tcccgcattt accgtcatgt catgtgacaa catgccagaa aacggtcatg ttatgcgtga
     1681 cgttgtcact tcctacgcgc aagccgttga tgtaaaactg gcacaatgga tcgaagataa
     1741 cgtgactttc ccatcaacaa tggtggaccg tattgtgccc gcagtgacag aggatacgct
     1801 ggcgaaaatc gaacaactta ccggtgtgcg cgatcctgct ggcgttgcct gtgaaccttt
     1861 ccgccagtgg gttatcgaag ataactttgt agccggacgt ccggaatggg aaaaagcggg
     1921 agccgaactg gttagtgatg tgctgcctta tgaagagatg aagttgcgca tgctcaacgg
     1981 cagtcattca ttcctggcat atctgggtta tcttgctgga tatcagcaca ttaatgactg
     2041 tatggaagat gaacattatc gtcatgcggc gtatggcatg atgttgcagg aacaagcgcc
     2101 gacgctgaaa gtgcagggcg ttgatttgca agattacgct aaccgattaa ttgcacgcta
     2161 tagcaacccg gcgttacgtc atcgaacctg gcagattgcg atggatggca gccagaaatt
     2221 gccacagcgg atgttggatt ctgttcgctg gcatctggcg catgacagca agttcgatct
     2281 gctggcgctg ggcgtcgcgg gttggatgcg ttatgtcggt ggtgttgatg aacagggaaa
     2341 tccgatagaa atcagtgacc cactgttacc tgttattcag aaggctgtac aaagtagtgc
     2401 cgaagggaaa gcgcgcgtcc agtcattgct ggcgattaag gcaatctttg gtgatgattt
     2461 gccagacaat agcttgttta ctgcaaaagt gacggaagcg tacttgtctt tattagcgca
     2521 tggcgcgaaa gcgaccgtgg cgaaatattc cgtgaagtaa aaaaagcaat gccgccatcg
     2581 gcggcattca cacacttagt tcatgccgag acgaatcagg tccagaggtg ccagtttctc
     2641 ttcacaacct tcaacaacta cggtttttcc gcggcggatt tgctcagcgg tcagaccttc
     2701 agtatcaatc gacaggatct tgccagtgtg accggtgccg ctaaccataa cacggctgcc
     2761 agtggtgatt gcgttacggt tacgatcgta ggtcgtcata ggtgttttct cctttctgat
     2821 ttacagtcga agcccacgta acgggcgcaa tataaatact cctgtcgagt gaaatatttt
     2881 ttgtttttga tcaagctcac atcttttttg atgcatgcat gagccgtaag gcggggggaa
     2941 aagcctctca tcccggatgg gatgagagta aatgaattac tcttcgctaa accagtcgct
     3001 gttttcctgg cggatctggc gcacggattc gctgatttcc tgcaaatgtt gcgtcattgc
     3061 acgttctacc gcatcgccat cacgtttttg cagggcagag aaaatatcaa gatgctggcg
     3121 taacagcatt tctggtggag aaacatggtc gaaactcata tagcgcacgc gatcaacggt
     3181 cgctttcagg ttctcaatgg tatcccacgc cagttgacag tcggcaatct gcgtcaggag
     3241 ttgatggaag ttgtcatcaa gttcaaaaaa atcatccagt tgcttgcgct caatggcaat
     3301 gcgttgctgg tgaagatttt gttccagttg gtagcactgg ctttcggtaa tcatgctcgc
     3361 cgcccgacgc gccaccgcgc actcaatggc ctgacggata aaactgccgt tgcgcacctg
     3421 ggccatggaa attttgttga cgtagctgcc acgctgcgga cgaatttgaa tcaggccgtt
     3481 ttccgccagt ttaataaagg cttcacgaac cggctggcgt gacacattga aacgaacaga
     3541 aacttctttt tccgacaacg gtgtgcctgg tgcaatcagg caatggacaa tgtcgcgacg
     3601 aagaatacga taaatctgct gattgacagg ctgtgtggga ttaagttgcg tttcgacggt
     3661 cattcgttct tgcttgtgag tgagttaact gcatgagtct accacttttt tgtgacaagc
     3721 aataacccgg cagttacgcc gggtataaaa attactgacg gtggacattc agtccggcat
     3781 agctttgggt aaccggcatc atttccaggg tattgatatt gacgtgagca ggcagcgttg
     3841 acacccacca gacggcttcg ctgacatctt ctggcgtcaa tgcaacggta ttttgatagg
     3901 ttttttccgc tttaccgtca tcgcctttaa agcggacatt ggaaaactcg gtgccaccca
     3961 ccagacccgg ttcgatgtcg gtgacgcgca ccgccgtacc atgcagatcc gtacgcagat
     4021 tcaggctaaa ctgacgaaca aacgctttcg tcgcaccgta aacgttacca ccggcatacg
     4081 gccagctgcc tgccgttgag ccaatgttaa taatatgacc atgattacgt tcaaccatac
     4141 ccggtaagac ggcgcgcgtc atatatacta ggcctttgtt gttggtatca atcatcgttt
     4201 cccagtcttc aacgctggct ttatgcgcag gctccatgcc caacgccagg ccggcattat
     4261 ttaccaggat atcaatattg ctccactcgg cagggagcga tgccagcatc tcttcaatag
     4321 cggcgcggtt gcgaacgtcc agttgggcga tatacagatt atctcccagt tcgtctgtta
     4381 actcctgcaa ccgctcctgg cgacggccag tggcgataac tttatgccct tgttgaataa
     4441 aacgacgagt aatgcattca ccaaaacctg ccgttgctcc agttactaaa acgatcatct
     4501 cactgttcct caacgctttt gtgtgtcaat aacatagcac gtgatgctta agagcgggta
     4561 atactggttt gcaagattgc actccgttat cagtaagcct acgctatgga acacctcgcg
     4621 ttcaggagaa gaaaatgaca acaatgaatc ctttccttgt gcaaagcaca ctgccgtatc
     4681 tggctcctca ttttgatcaa attgccaatc atcactatcg cccggcattc gatgagggaa
     4741 tgcaacaaaa gcgggcagaa attgctgcca tcgcgcttaa cccgcaaacg cctgatttca
     4801 acaatactat tctggcactg gaacaaagcg gagaattaat tacccgcgtt accagcgtct
     4861 tttttgcgat gactgcggcg cataccaatg atgaattaca gcgtcttgat gaacagtttt
     4921 ccgctgaact ggcggaactg gctaatgata tctatctgaa cggtgaatta ttcgcgcggg
     4981 tagatgctgt ctggcagcgc cgtgaatccc tggggcttga tagtgaatcc atccgcctgg
     5041 tggaggttat tcatcaacgt tttgtccttg ccggagccaa acttgcacaa gctgataaag
     5101 caaaattaaa agtactgaat acagaagctg cgaccctgac cagccagttt aaccagcggt
     5161 tacttgcagc aaataaatcc ggcggtctgg ttgtgaacga tatcgcgcag ctggcaggaa
     5221 tgagtgagca agagattgcg ctggcggcag aggcggctcg cgagaaaggt ctggataaca
     5281 aatggctgat tccgctgctg aataccaccc aacaaccggc gcttgccgaa atgcgcgatc
     5341 gtgcgacgcg tgaaaaactg tttattgcgg gctggacgcg agcggaaaaa aatgatgcca
     5401 atgatacccg cgctatcatt caacgtctgg tagagattcg cgcacagcag gcgaaactgc
     5461 ttggttttcc tcattatgcc gcatggaaaa tcgccgatca gatggcaaaa acgccagaag
     5521 cagcactcaa ctttatgcgg gaaattgttc cagcggcgcg tcaacgtgcg agcgatgaat
     5581 tagcctccat acaggcggtt atcgataagc agcagggcgg gtttagcgcg cagccgtggg
     5641 actgggcatt ttatgcggaa caggtacggc gggagaaatt tgatcttgat gaggcgcagc
     5701 tcaagccata ttttgaatta aacacggtgt taaatgaagg tgtattctgg accgcgaatc
     5761 agctcttcgg tattaagttt gtcgaacgtt ttgatattcc ggtctaccat cctgacgttc
     5821 gtgtgtggga aatttttgat cataatggcg tgggactggc gttattttac ggtgatttct
     5881 tcgcccgtga ttcaaaaagc ggcggtgcat ggatgggcaa ttttgttgag caatcaacgc
     5941 ttaatgaaac gcatccggta atttataacg tctgcaatta tcagaaaccc gctgccggtg
     6001 agcctgcgtt gttactctgg gatgatgtaa taaccttatt ccatgaattt ggtcatacgc
     6061 tgcacggcct ttttgcccgc cagcgttatg ccacgctttc cggcaccaac acgccgcgtg
     6121 attttgtcga atttccgtcg caaatcaacg aacactgggc aacgcatccg caggtattcg
     6181 ctcgctacgc ccggcattat cagagcgggg cagcaatgcc tgacgaactg caacagaaaa
     6241 tgcgtaatgc cagcctgttc aacaaagggt atgagatgag cgaactgctt agcgccgcac
     6301 ttctcgatat gcgctggcat tgcctggaag aaaacgaagc aatgcaggat gtcgatgatt
     6361 ttgaattgcg ggcgctggtg gcggaaaata tggatcttcc tgctataccg ccacgctatc
     6421 gcagcagtta tttcgcccat atttttggtg gcggatatgc tgcaggttat tacgcttatc
     6481 tgtggacgca aatgttggcc gatgatggtt accagtggtt tgttgagcag ggcggattaa
     6541 cgcgtgaaaa tgggcagcgt tttcgcgagg cgatcctttc cagaggtaac agcgaagatc
     6601 tggaacgcct gcatcgacaa tggcgtggta aggctcctca gattatgccg atgctgcaac
     6661 atcgtggctt gaacatataa acaccaccaa tggctggcct ggagatatcg ccaggccagc
     6721 ctggttatag cagtaattgc agcagctggg cgagggcaaa cctcaccgct aaagctaata
     6781 ccgtttcgca gtcgccagca aaatgcataa ccgcagtgta gttctggcct ttaatatgcc
     6841 acgcgaacca gacggtaccc gctggcgtac catcttcacc gccctccggt ccgccgtagc
     6901 cggtaatggc aatactgaca tcagcatccg cacgctctat ggcacctgtt gccatttctg
     6961 ctgccacttt ctcactcacc gcagaatatc gttcaagaga ctgctggctt acgctgagga
     7021 ttttcatctt tgcctgatcg gtgaaagtaa caaagcctgc accgtaaaat ttgggtgtat
     7081 cttcagctgc acacagggcg ctagccaact taccgccggt gcatgattcg gcggtagtta
     7141 agcgtaattg atccgcgata agtctttgac tcaacgcgga tgtcaggttt tcaatagtgt
     7201 cggtatctgc gagttgtaca attttatctt tattaatgtt cactgttttt tgtccacgct
     7261 attatatgta aaggtgattt gctttttctg tatgctcgtc caatataata attttcaaac
     7321 atataaatcc gattattctg aaaacatgtt taagactctt caatcttctt aactttagtg
     7381 agtcaaattc tctgaatatt tgacagctag tgggctgatt ttaaatgcct tataacaatt
     7441 ggcagtattc ttatttggca attataaaaa tagcaaccag acttaataag tcaaaccaaa
     7501 cgcaattaag gagttagtat gaaatttcag tccattgtat tagcaagttt tcttgtcatg
     7561 ccttatgcgt tggcagatga ccagggcggt ttaaaacagg atgcagcgcc accgccgcct
     7621 catgcaatag aagatggcta tcgcggaacc gatgatgcaa aaaaaatgac cgttgatttc
     7681 gcaaaaaaca tgcacgatgg cgcttcggtt tcactacgcg gtaatttgat ttctcacaaa
     7741 gg
//
LOCUS       DAJTQQ010000154         7541 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000154 DAJTQQ010000000
VERSION     DAJTQQ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.154"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            1..1160
                     /gene="rbsA"
                     /locus_tag="OO309_003809"
                     /pseudo
     CDS             1..1160
                     /gene="rbsA"
                     /locus_tag="OO309_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1165..2130
                     /gene="rbsC"
                     /locus_tag="OO309_003810"
     CDS             1165..2130
                     /gene="rbsC"
                     /locus_tag="OO309_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR5848773.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2155..3045
                     /gene="rbsB"
                     /locus_tag="OO309_003811"
                     /pseudo
     CDS             2155..3045
                     /gene="rbsB"
                     /locus_tag="OO309_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3171..4100
                     /gene="rbsK"
                     /locus_tag="OO309_003812"
     CDS             3171..4100
                     /gene="rbsK"
                     /locus_tag="OO309_003812"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR5848774.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4104..5096
                     /gene="rbsR"
                     /locus_tag="OO309_003813"
     CDS             4104..5096
                     /gene="rbsR"
                     /locus_tag="OO309_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR5848775.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5062..6489)
                     /gene="mdtD"
                     /locus_tag="OO309_003814"
     CDS             complement(5062..6489)
                     /gene="mdtD"
                     /locus_tag="OO309_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR5848776.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6512..7204)
                     /locus_tag="OO309_003815"
     CDS             complement(6512..7204)
                     /locus_tag="OO309_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR5848777.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1918 a         1830 c         1986 g         1807 t
ORIGIN      
        1 aaaaccatgt atgtcgaagc ggataaattg ctggctaaac ttaacctgcg ctttaaaagc
       61 gacaagttag tgggcgatct ttccatcggt gaccagcaaa tggttgaaat cgccaaagtg
      121 ctgagctttg agtcgaaagt catcattatg gatgaaccga ccgatgcgct gaccgatacc
      181 gaaaccgaat ccctgttccg cgtcatccgc gagctgaaat cgcaaggcca ctgtattgtc
      241 tatatctccc accgcatgaa agaaatcttc gagatttgcg atgacgttac cgtttttcgt
      301 gatgggcaat ttattgctga gcgcgaagtg gcatcactga ccgaagattc gctgattgag
      361 atgatggtgg gccgcaagct ggaagatcaa tatccacacc tggacaaagc gccaggagat
      421 atccgcctga aagtcgataa tctctgcgga cctggcgtta acgatgtctc ttttacttta
      481 cgcaaaggcg aaattcttgg cgtctctggt ttgatgggcg caggtcgtac cgaactgatg
      541 aaagtgctct acggcgcact gccgcgcacc agcggttacg tcaccctgga tgggcatgaa
      601 gtcgttaccc gttcaccgca ggatggcctg gcaaacggca ttgtgtatat ctccgaagac
      661 cgtaaacgtg acggtttagt gctgggcatg tcagtaaaag agaacatgtc gctgacagcg
      721 ctgcgctact tcagccgcgc tggcggcagt ttgaagcatg ccgatgaaca gcaggctgtg
      781 agtgatttca ttcgtctgtt taatgtgaaa acgccgtcga tggaacaggc aattggtctg
      841 ctttccggtg gcaatcagca aaaagtggcg attgcccgcg gtctgatgac acccccaaag
      901 tattgatcct tgatgagcct acccgtggcg tagatgtcgg cgcgaaaaaa gagatctatc
      961 aactgattaa ccagttcaaa gccgatggct tgagtatcat tctggtgtca tcggagatgc
     1021 cagaagtatt aggcatgagc gatcgcatca tcgtcatgca tgaagggcat ctcagcgggg
     1081 aatttactcg tgagcaggcc acccaggaag tgttaatggc tgtcgctgtg ggcaagctta
     1141 atcgcgtgaa tcaggagtaa aaaaatgata acccagactg tctctggtcg ccgttatttc
     1201 acgaaagcgt ggctgatgga gcagaaatcg cttatcgctc tgctggtgct gatcgcgatt
     1261 gtctcgacgt taagcccgaa ctttttcacc atcaataact tattcaatat tctccagcaa
     1321 acttcggtga acgccattat ggcggtcggg atgacgctgg tgatcctgac gtcgggcata
     1381 gacttgtcgg taggttctct gttggcgcta accggcgcag ttgctgcatc tatcgtcggc
     1441 attgaagtca atgcgctggt ggctgtcgct gctgctctca cgttaggtgc cgcaattggt
     1501 gcggtaaccg gggtgattgt agcgaaaggt cgcgtccagg cgtttatcgc tacgctggtt
     1561 atgatgcttt tactgcgcgg cgtgaccatg gtttatacca acggtagccc agtgaatacc
     1621 ggctttactg agaacgccga tctgtttggc tggtttggta ttggtcgtcc gctgggcgta
     1681 ccgacgccag tctggatcat ggggattgtc ttcctcgcgg cctggtacat gctgcatcac
     1741 acgcgtctgg ggcgttacat ctacgcgctg ggcggcaacg aagcggcaac gcgtctttct
     1801 ggtatcaacg tcaataaaat caaaatcatc gtctattctc tttgtggtct gctggcatcg
     1861 ctggccggga tcattgaagt ggcgcgtctc tcctctgcac aacccacggc ggggactggc
     1921 tatgagctgg atgctattgc tgcggtggtt ctgggcggta cgagtctggc gggcggaaaa
     1981 ggtcgcattg ttgggacgtt gatcggcgca ttaattcttg gcttccttaa taatggattg
     2041 aatttgttag gtgtttcctc ctattaccag atgatcgtca aagcggtggt gattttgctg
     2101 gcggtgctgg tagacaacaa aaagcagtaa taacgactac aggacatctt gaatatgaac
     2161 atgaaaaaac tggctaccct ggtttccgct gttgcgctaa gcgccaccgt cagtgcgaat
     2221 gcgatggcaa aagacaccat cgcgctggtg gtctccacgc ttaacaaccc gttctttgta
     2281 tcgctgaaag atggcgcgca gaaagaggcg gataaacttg gctataacct ggtggtgctg
     2341 gactcccaga acaacccggc gaaagagctg gcgaacgtgc aggacttaac cgttcgcggc
     2401 accaaaattc tgctgattaa cccgaccgac tccgacgcag tgggtaatgc tgtgaagatg
     2461 gctaactagg cgaacattcc ggttatcact cttgaccgcc aggcaacgaa aggtgaagtg
     2521 gtgagccaca ttgcatctga taacgtactg ggcggcaaaa tcgccggtga ttacatcgcg
     2581 aagaaagcgg gtgaaggtgc caaagttatc gagctgcaag gcattgccgg tacatccgca
     2641 gcccgtgaac gtggcgaagg cttccagctg gccgttgctg ctcacaagtt taatgttctt
     2701 gccagccagc cagcagattt tgatcgcacc aaaggtttga acgtaatgca gaacctgttg
     2761 accgctcatc cggatgttca ggctgtattc gcgcagaatg atgaaatggc gctgggcgcg
     2821 ctgcgcgcac tgcaaactgc cggtaaatcg gatgtgatgg tcgtcggatt tgacggtaca
     2881 ccggatggcg aaaaagcggt gaatgatggc aaactagcag cgactatcgc tcagctaccc
     2941 gatcagattg gcgcgaaagg cgttgaaacc gcagataaag tgctgaaagg cgagaaagtt
     3001 caggctaagt atccggttga tctgaaactg gttgttaagc agtagtttta atcaggttgt
     3061 atgacctgat ggtgacataa atacgtcatc gacagatgaa cgtgtaatat aaagaaaagc
     3121 agggcacgcg ccaccctaac acggtggcgc attttatgga catcccgaat atgcaaaacg
     3181 caggcagcct cgttgttctt ggcagcatta atgctgacca cattcttaat cttcaatctt
     3241 ttcctactcc aggcgaaacc gtaaccggta accactatca ggttgcattt ggcggcaaag
     3301 gcgcgaatca ggctgtggct gctgggcgta gcggtgcgaa tatcgcgttt attgcctgta
     3361 ctggcgatga cagcattggc gagagcgttc gccagcagct cgccactgat aacatcgata
     3421 ttactccggt cagcgtgatc aaaggcgaat caacaggtgt ggcgctgatt tttgttaatg
     3481 gcgaaggtga gaatgtcatc ggtattcatg ccggcgctaa tgctgctctt tccccatcgc
     3541 tggtggaagc gcaacgtgag cgtattgcca acgcgtcagc gttattaatg cagctggaat
     3601 caccactcga aagtgtgatg gcagcggcga aaatcgccca tcaaaataag actatcgttg
     3661 cgcttaaccc ggctccggct cgcgaacttc ctgacgaact gctggcgctg gtggacatta
     3721 ttacgccaaa cgaaacggaa gcagaaaagc tcaccggtat tcgtgttgaa aatgatgaag
     3781 atgcagcgaa ggcggcgcag gtactgcatg aaaagggtat ccgtactgta ctgattactt
     3841 taggaagtcg tggtgtatgg gctagcgtga atggtgaagg tcagcgcgtt cctggattcc
     3901 gggtgcaggc tgtcgatacc attgctgccg gagatacctt taacggcgcg ttaatcacgg
     3961 cgttgctgga agaaaaacca ttgccagagg cgattcggtt tgcccatgct gccgctgcga
     4021 ttgccgtaac acgtaaaggc gcacaacctt ccgtaccgtg gcgtgaagag atcgacgcat
     4081 ttttagacag gcagaggtga cgcttggcta caatgaaaga tgttgcccgt ttggcgggcg
     4141 tttctacctc aacagtttct cacgttatca ataaagatcg cttcgtcagt gaagcgatta
     4201 ccgccaaagt tgaagcggcg attaaagaac tcaattacgc gccatcagct ctggcgcgta
     4261 gcctcaaact caatcaaaca cataccattg gcatgttgat cactgccagt accaatcctt
     4321 tctattcaga actggtgcgt ggcgttgaac gcagctgctt cgaacgcggt tatagtctcg
     4381 tcctttgcaa taccgaaggc gatgaacagc ggatgaatcg caatctggaa acgctgatgc
     4441 aaaaacgcgt tgatggctta ctgttactgt gtaccgaaac gcatcagcct tcgcgtgaaa
     4501 tcatgcaacg ttatccgaca gtgcctaccg tgatgatgga ctgggctccg ttcgatggcg
     4561 acagcgatct tattcaggat aactcgttgc tgggcggaga cttagcaacg caatatctga
     4621 tcgataaagg tcatacccgt atcgcctgta ttaccggtcc gctggataaa actccggcgc
     4681 gcctgcggtt ggaaggttat cgggcggcga tgaaacgtgc gggtctcaac attcctgatg
     4741 gctatgaagt cactggtgat tttgaattta acggcgggtt tgacgccatg cgccaactgt
     4801 tatcacatcc gctgcgtcct caggccgtct ttaccggaaa tgacgctatg gctgttggcg
     4861 tttaccaggc gttatatcag gcagagttac aggttccgca ggatatcgcg gtgattggct
     4921 atgacgatat cgaactggca agctttatga cgccaccatt aaccactatc caccaaccga
     4981 aagatgaact gggggagctg gcgattgatg tactcatcca tcggataacc cagccgaccc
     5041 ttcagcaaca acgattacaa cttactccga ttctgatgga acgcggttcg gcatagattt
     5101 acgctgtctt ttgatcaaat tattaccatc ggttgttttc agaagcatga acattgctgc
     5161 tgaagcaaca gtaataatgc ccatcgtgat aaacgtatag tggaattgtt cgacagtcgt
     5221 tgtgccttcc atcccttcat aaatgcgaag gacggccgca cttacagcaa cgcctaaact
     5281 aatcgacagt tgctgcgtga ccgccagaac actgttaccg ctgctggcgt tgtcatcggt
     5341 cagatcggca agtgtgatgg tattcatcgc ggtaaactgc gtcgacatag ccatccctaa
     5401 tataaacaac ggcaagatca gcatccatat cgccattgcc ggtgattgca aagagaactg
     5461 agcgatcatt agcccaataa tcaccgtgat ccccactaat gtatggcgat agcccagacg
     5521 acgtaagact tgggtaacca tcgattttgc aataatggaa cctaacgctg tcggtgccat
     5581 catacagccg gcaataaacg cctgataacc aaatcctacc tgtaacatca atggcataag
     5641 gaacggtaca cagccggtcc ccagacgggt tgcaatattg cctacgatac cgatcgagaa
     5701 agtgcgggtt ttaaataaat ctaatgaaat taatgggttt ggcgtacgtc gcgcatggag
     5761 aatatagaga agcagtaacc cgatgctggt gacaattacc gtcaaggcaa tccagctggc
     5821 gacaatcttt tccccgaata gctctattcc gcttgaaaag agaacaaggc tgaggccaaa
     5881 cagcaaaaag ccagtgatat cgaatctgcg tcgtgcggtg gtgaaattgg gcatatgttt
     5941 gcgcgcgtaa agaaggcccg caatacctat ggggatatta attaaaaata tccagtgcca
     6001 ggttgcccag gtgaccagca cgccgccaag aacggggcct aaaattggcc ccaccagacc
     6061 cggcatggcg acaaaattca ataccggaag aagttcatta cgaggataag cacgcagtaa
     6121 ggccggccga gcaacaggca tcatcattgc gtcgcctatc ccttgaataa cccggaagac
     6181 aaccagctgt ggtagcgaat tagaaagtgc gcaggccaga gaacccaacg tgaacagact
     6241 cacggcaagg gtaaaaatgc gacgcgtacc gaagcgatcg gctagccatc cgcttaccgg
     6301 aataagcatc gccaccgtca gcgtataact gatgatggct gattgcatcg cgagaggaga
     6361 acgattaagg ctatgagcga ttgcgggtaa ggcggtattc agaatagtgg catcaagtgc
     6421 ctgcatgaag aaggccatcg ccgcgatcca cggcaaaccc gacatactac gcttcttttt
     6481 atcgctcatt caatgtcctg ttatcgggtt atcacttatc aggtgagcgt agcagcgcct
     6541 gacaagcttt aaatgccgcg tcgccatcgc tttggataat cgcatcgaca atcgcctggt
     6601 gcagatccag ctttatcact gtgtcgctgg taattgacgt gaagtaagtg tgataaaccg
     6661 aatggaatag cgaggcgaat gaggtcaaaa acggattggc gctcatttca tagatatgct
     6721 catgccaggc catatcgact tcgatccagc gttcacggcg aaagttctct tttaatgccg
     6781 ccatttcggc cattaacgta ttgagatgcg ccttctgttc cgcggtgcca accgttgctg
     6841 ccagtaggca ggcttgcggc tccagacaga tacgcataac cagaaagtga tcgatgacct
     6901 gatgaaagtt ctcttctgtt atccaccaag taagcaattc ctgatcaaga aaattccagt
     6961 tcgattgtgg catgacccga gtaccaattc gcggtcgcgg taaaaccatc ccttttgccg
     7021 ttaacgtttt gaccgcttcg cgtaccgctg tacgactcac tccaaattgc tcgcccagct
     7081 caatctcacc gggcaaaatg gtgccgggtt catattcacc ttttaagatc cgttgcgcca
     7141 gcttctcagc cagaacatac gaaaggtttt tctgtgcagc taactgttgt gcgcttaaag
     7201 gcattactta tcttcctttt tctttttatt cctccttagt atgccaccag gaagtgtgat
     7261 tacggttgca aaaacggcaa attgcttgtt ttatggcaca ttaacggggt gttttgctga
     7321 aaaaatgcgc ggtcagaaaa ttattttaaa tttcctcttg tcaggccggg ataactccct
     7381 ataatacgcc accactgaca cggaacaacg gcaaatacgc cgccgggtca gcggggttct
     7441 cctgagaatt ccgacagaaa aagcaaaaat aaatgattga ctctgtagcg ggaaagcgta
     7501 ttatgcacac cccccccccg cgccgctgag aaaaagcgaa g
//
LOCUS       DAJTQQ010000155         7397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000155 DAJTQQ010000000
VERSION     DAJTQQ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.155"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..193
                     /locus_tag="OO309_003816"
     CDS             <1..193
                     /locus_tag="OO309_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848778.1"
                     /translation="YGMRFPMTFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITV
                     SPGSGYGSSADAYYIAMGY"
     gene            165..599
                     /locus_tag="OO309_003817"
     CDS             165..599
                     /locus_tag="OO309_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5848779.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1372..2421)
                     /locus_tag="OO309_003818"
     CDS             complement(1372..2421)
                     /locus_tag="OO309_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5848780.1"
                     /translation="MYNNKIDSNRKIRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSVFDYIKSKFYRLMIPYFTFAFSILIINTINSGETI
                     DYIYNHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFREIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
     gene            2872..4062
                     /locus_tag="OO309_003819"
     CDS             2872..4062
                     /locus_tag="OO309_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HCR5848781.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(4452..5912)
                     /gene="gtrII"
                     /locus_tag="OO309_003820"
     CDS             complement(4452..5912)
                     /gene="gtrII"
                     /locus_tag="OO309_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HCR5848782.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYNFYNNPPTGLNLYKWLLIPLLILMFT
                     LTYKLKTRSIYLISSIIFIYILPVIFIVVVGSGAPPRLFVLMPIVAVILFSCLSNFRS
                     IKYLNCMFFLFIIFNGVSTSKNLFLNDTLARQKDISLAKEISYTSQTKGISLNGKYIY
                     IYGSNDSGNMLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQIEKIYP
                     IVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
     gene            complement(5909..6838)
                     /locus_tag="OO309_003821"
     CDS             complement(5909..6838)
                     /locus_tag="OO309_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR5848783.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            complement(6835..7197)
                     /locus_tag="OO309_003822"
     CDS             complement(6835..7197)
                     /locus_tag="OO309_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR5848784.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
BASE COUNT         2326 a         1380 c         1456 g         2235 t
ORIGIN      
        1 atacgggatg aggttcccga tgacgttctc aagagtaatt gctgtcgtag ttacattgaa
       61 cgttactggc gcggctgggc agccgactgt atcggcgaca agtgtccaga acactggatt
      121 taatattact gtgtcgcccg gttcaggata cggttcatct gctgatgcgt attacattgc
      181 aatgggatat taacaaaatg tcatattttt attctgcatc gacaaacgga ttttattcga
      241 ctgaatttca cggcaccaat attcctgatg atgcagtgga aatctcggaa tcagagtgga
      301 aaacactgat taatgcacag agtgtaacaa aaatgattac ctgtggtgag aacggtcatc
      361 ctgtcattgt tgaccgtcct tctccaacac cagaacaatt agccttaata aatgatgaaa
      421 agaaatctgc actgatagca gaggcaacga atgtaatagc tccgcttcag gatgcggttg
      481 atttaggtat ggcaacagat gatgaaacga aactgttact ggcatggaaa aaatatcggg
      541 tgctgcttat gcgtgttaat gtagtaaaac ccgagtggcc tatgcatcca aataaataga
      601 cttgttaatc taggataatt gttggaataa tactcgtttt tcagtaggtt caggtagcct
      661 gggtataatg ctcacttcta caatgatttc aacttatttt atttaattga tattacgaaa
      721 cttaactcat acataaacaa tatgactcaa tactgtccta aatagctgcg cagaaaagtg
      781 ctccactttc gctcagccct tgctgggcga agcatcagga aggcgttctc agctaggttg
      841 tgttatatct ggcttgagtt ttgttgccca tacgcaacac attacccatc tgtaaacgac
      901 aagtgtggca gaattaggtg cagctattta gactatatcg acgcagtaac gataatagat
      961 atcgaaactg tactagatat tagctactta tggttaccgg ggggagtctt ttgagcactg
     1021 gtgttatcaa ttggcttcaa ataattcgcc aataaattat attgcgatag ctcatcacct
     1081 tccaggttgt ttatagccag tttatcagcg tcaccaccta ctgtttatgc ttttatttct
     1141 ttgattaata agtatatgat attttaattc atctggtgta tttctttctc atgtgttggt
     1201 cgactgaata tctgttcagt gaaggggtaa ttttatgttt gcacttcgaa cattcccttc
     1261 agttgctagg aaagaaaacc gtaacatttg cgcattaatt ttaacagtat aatctgagca
     1321 taaatatgcg ctatatatct ataaaaaggc gcaattaatt gcgccttttt atcattttat
     1381 caaaaatggc ccaccgactg tcctcgatag ccaatggcca attttatctg atacatatac
     1441 acaaatacag gtatatatga ttgcgcagat aggatttgga atagtcacgc tgaagcctat
     1501 aattgctaaa gccataggga taaataattt ggtgatatac tccattccac atatgttaag
     1561 agtatttctt ccagctctta caataaagtc atttccacaa attatttttg ctatgaaaat
     1621 attaaagatt atcaagccta ccgtggtgat aatagtatta atagcttgaa tataactact
     1681 tgatattatg tctgcattga atattgagat aatcttaaaa agtaaaggtt tctggttaag
     1741 tagttgatac gcactcatta tagcggtaat tgagaagaca atgaaaccaa ttttagattt
     1801 tccaaaaaac ctgtcacgtg ttaattctaa aaacatacat ctaccgagag gcaataacca
     1861 ccagtatgcc atagcggagt ctatgttcca aaaccattga ggatctagta atgggttatg
     1921 attgagcact gtttgtgaca acatgaacga taacagtgat atgattaaaa tcactatatt
     1981 gttcttgact atttctctaa agattgcatc aattgctatt ataacaaaaa ggcagtttat
     2041 aaaccatatt gttcctacga attggttatt tctaacacca tatattatat cataaatgtg
     2101 gttgtatata taatcaatcg tctcacctga gttaattgtg tttattatta atatagaaaa
     2161 tgcaaaagtg aaatatggaa tcattaatct gtaaaacttt gacttgatat aatcgaaaac
     2221 agataagtca ttttttttga tagtaaaaaa accagcagcg aaaaagaata gtgggacgtg
     2281 ataactaaaa acaaaaggat acaatttgcc agcccccaac ccaaggtgac ctaaatatat
     2341 agcaaaaata ccgagaaatt ttaaagcatc aacccaatca tgtcttattt ttctgttgct
     2401 gtcgattttg ttattataca tttaaagtag gctctccagt tttgtgtgat ttttaaacac
     2461 tcattgcaaa tatgaatgcc tgaaagttta tgattattaa tcaacgagtt caatattaat
     2521 ttattatttt tcaaagtcgc actatatagg catttacttc cgcagtacca ccgatagtat
     2581 cgatatcaaa tcagcctgcg gccgcgataa ctttcgtaac attctgccag cggctttgtt
     2641 gaataaatca ggttcaagat aatcgtctct gcatcaggtt agagtttcag gcaatacatc
     2701 ctctttctgg catacctgcc actctcgcac catggtcata gcctttacga tctgcatact
     2761 caccagacca gagcccgctc ccttttaggc tgaggaatcc ccagaaaaga agacaggcat
     2821 agagttttgt gatctactga ttgcgctaac aaattcaatg agatcacctc tatgcctgcc
     2881 cgtaaagtat gccagacttt tttccgtaat gctttagctt ccacgcatca gtatcgacaa
     2941 aatgccatta ttgattctgc tgctgcttta gtcggtggcg catctctttc tcttaccagc
     3001 attggccggc atttacccgg tcctgctcgc gtgaaagata aaataaaacg cgttgactgt
     3061 ctgttgggta ataaacgact tcataatgat atccctctga tattcaaaaa tattacatcc
     3121 atgatgacaa ataaactgtc gtggtgtgtt atcgctgttg actggagcgg ttaccctttt
     3181 caggaatatc atgtccttcg tgccagcctc ctctgcgacg gtcgctctat ccccctgatg
     3241 agtcaggtct tcccgtcaaa aaaaaataac gaggcggtgg aaatcgcttt tctggatgcg
     3301 ctttccgggg ccatttctcc ccggactcgt gttgttattg ttactgatgc tggttttcaa
     3361 agtgcatggt tccgtcatat caaatcacag ggatgggatt ttattggtcg tatcaggggg
     3421 gtagtgaagt tcaggctgga tagtgacaaa gataagtggc tggacataaa aatgtgcagg
     3481 gggagttcag aggcaaaata cctggggacg ggaaccctgg cccggaaaaa acgctcgcag
     3541 tgtgaaggac atttttatct ttataaacat tcacctaaag gcagaaaaag caggcgtgcc
     3601 agagggaggc cgggcttacc aacaacggaa aaagaacaaa aagccgcagg cagagaacca
     3661 tggttaatat tcagcaatac agctgagttt aatgccaaaa aaatcatgaa actgtacagt
     3721 cgaagaatgc agatagagca aaactttcgg gatgaaaaaa gtgaacgctt tggttttggc
     3781 cttcgggcga gcagaagtcg gaggggagag cgttttctgg tattaagtct actggttaca
     3841 ctggccagta tcgtactctg gctacttggt tatcattttg aaaataaagg atttcattta
     3901 aagtatcagg ctaatagtct caaaaaacga agagtattgt cttttctgac gctggctgaa
     3961 aacgtcctga gatttaaccc tgaacttcta cgacgagcac aacctgaaaa gatattgtgc
     4021 cggttggcca gcacttaccg aagtatggtg ctggcttact gatgaaaatt ttctggggat
     4081 ccctcagcct ttcggggagg aatgagggct ctgatattct tttgccgtag ttcatcgtga
     4141 aaccgtcgga tgtcatttgg cctgagtgac ccatgcctcc caaaagcaga gtcgctagtg
     4201 ttatgtaatt aggcggttaa acttcaattc tcagtgttaa gtttgggtaa acgcttgtaa
     4261 atggtattgc taatttttta attgactaat attagaaacg gttagtgtga ttgtatatag
     4321 atatttgagt gtaaggtatt taaaggggca ttcagcccct ccgtctattt gtgattgcat
     4381 ccattgatgt gtgtattatt ttgagcttga aaaagggagg cgctctttaa taataaataa
     4441 atctatcaaa gctaaatatt aaatggaagc cacccttttt tctctgatag ttttattatc
     4501 accaaaccat ttatttcagc tatcgaatct ggattcggcc aggaaggtaa actcttcaca
     4561 attggataaa tcttctctat ttgttcttta tttgctggtt tgcaattaca gattccatag
     4621 taattcataa atgcaaccat cctaaaatag ttgccaccat cccaccaaaa aaaagatttt
     4681 ccaaaagtgt ctgcgctcat ggagagcata tttcctgagt cgtttgaacc atatatatat
     4741 atatattttc cgttaaggga aatgcctttt gtttgagatg tgtatgatat ttctttagct
     4801 aaagagatat ctttctgtct tgcgagagta tcattcaaaa atagattttt ggatgttgaa
     4861 acgccattaa atataataaa taaaaaaaac atgcagttta ggtattttat agagcgaaaa
     4921 ttgcttaagc aagaaaacaa aattactgct actataggca ttaaaacaaa caggcgaggt
     4981 ggcgcccctg agccaacaac aacgataaat ataaccggaa gtatataaat gaaaatgatt
     5041 gatgaaatca aataaattga tcttgttttt aatttatatg ttagggtaaa catcagaatt
     5101 aataaaggaa tcagtaacca cttgtatagg tttaaaccag tgggaggatt gttatagaag
     5161 ttatatatat tatctatggc tgatttaaca agccatttaa tatcgcttgc attttgtata
     5221 taatttgaga tgtactcgtt cgattcaagc gaaaaataat gcttgatagc tttggttaat
     5281 agcaggtaaa ttaaggtaga taaagctatg agggatagag ataaacatga actataaaaa
     5341 gattcacgaa tatttttctc atttcgtatt atcgaattta tctgcaaccc aatgacgaat
     5401 gcaataatat atgttacgaa tgtttggtag attgccattg aaagaatgga cagtactata
     5461 ccagaaaata tcacaatcct atttttttgc gagtgaaaaa taattgctga tatcgctgcc
     5521 agtagaaaag caattcccac agtatcagct tggttaagaa actctaattg ataggagatc
     5581 tgagggaagg taataaatac taacatacct atcaataatt cataagattc tagctttagc
     5641 gatctgcaga ttataaatgc tgaaatgatg ataaaagata aggctattaa tggtgttata
     5701 taaagtgaaa aaggctcagg gagaaagtaa tgtcgcaaaa aagtgtgaaa ccaccgccca
     5761 agcgtgattg tttgataaaa attatttgta aactcgccat caatattaag tgttccatcg
     5821 tacaaccacc cccaataaca ataaattgat attgcaaaac atgatatgaa ggcaaggaga
     5881 tttgcatttt taaataaatt aattttaatc atgggttacc tcgatgagat ttttttatga
     5941 tgtatttggg tctatttttt acttcaatat agattctacc tatatattct ccgagaacac
     6001 caatcccgat cagttgcact ccacccaaga aaagtattga tacaagcagg gagggatacc
     6061 cgcgtactgg gttaccaaag acaagggtgt ctataatcat ccatgcacca tataaaaatg
     6121 aaatgcttgc aacaaacaag cctatataag tccatacgcg aagaggaaag gttgaaaaac
     6181 ttgtgatacc ttccagtgcc agattccata atttccagcc attaaatttt gagatgccag
     6241 caacacgctc tgcgcgtaca tattcaacga catccgtcgg accacccacc cagctcagta
     6301 tgcctttcat gaaaagattg cgctcaggca acagtttaat gttctccaca acctcacgag
     6361 acatgagtcg aaaatctccg acattttcct cgatctttgg ggtgcttatt ttgttgtgta
     6421 atttatagaa ccactcagct gtcttacgtt tcagtcgtcc atcagttgag cggtctgaac
     6481 gtttagcaag caccatgtca gcacctgcct gccacttttc aataagatga gggataacct
     6541 caattgggtc ttgcaggtcg acatcaatag gaattacagc atcgccgctt gcatggtcta
     6601 accctgcaaa taaggctggt tctttaccaa agttgcgtgt aaatgacagc ggaacaacta
     6661 gcgggtctga aacagccagc gcgttaataa ttgactctgt ggcatcttta cttccgtcat
     6721 ttatgaatac aatttctact tcatatggct tcaactcttg gaattcacgt accgttttat
     6781 agaaaacagg tatcgcttct tcttcattga agacaggaac gacaagagat attttcattt
     6841 cgcatcccta aagacaatga actttgaata gacgaaaccg cacaccagac tgatggcgga
     6901 gaaggtgata agagtgacta ttggaggaag tgagcattta tcagctgccc aaccaacaat
     6961 cacactcaat attcccataa atcccacgta taacatgtag cgcatcgctg tagtcaatgc
     7021 tttgaatgtg aatcttgcat tcgcgaagaa gctaaagctc acagccacta cgaaacctgt
     7081 gaagtttgcc agagcctgac tggtatgtgc ggcatagata catacaccaa aaaccaccca
     7141 gtgtataagt gtgttcagca cacctataga tgtgtacttt acaaataact ttaacattta
     7201 tttaatcaat gagctctgaa aggcatgaag tctatcatcc aagtctcaat tgatcgatac
     7261 ttgctatgtc tgatgagaca aaactgagac acataaggcc tcacaatggc ttgcaaggct
     7321 ttacatgttt tgatgtggtg ggacgtgtga gcgcagtgtt gatgggataa tcctttgaat
     7381 tacaagcgga ttcttat
//
LOCUS       DAJTQQ010000156         6959 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000156 DAJTQQ010000000
VERSION     DAJTQQ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6959
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.157"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(82..984)
                     /locus_tag="OO309_003823"
     CDS             complement(82..984)
                     /locus_tag="OO309_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCR5848785.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTLVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1063..2016)
                     /gene="lsrR"
                     /locus_tag="OO309_003824"
     CDS             complement(1063..2016)
                     /gene="lsrR"
                     /locus_tag="OO309_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCR5848786.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2265..3800
                     /gene="lsrA"
                     /locus_tag="OO309_003825"
     CDS             2265..3800
                     /gene="lsrA"
                     /locus_tag="OO309_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCR5848787.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            3794..4822
                     /gene="lsrC"
                     /locus_tag="OO309_003826"
     CDS             3794..4822
                     /gene="lsrC"
                     /locus_tag="OO309_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCR5848788.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            4822..5814
                     /gene="lsrD"
                     /locus_tag="OO309_003827"
     CDS             4822..5814
                     /gene="lsrD"
                     /locus_tag="OO309_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCR5848789.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            5826..6849
                     /gene="lsrB"
                     /locus_tag="OO309_003828"
                     /pseudo
     CDS             5826..6849
                     /gene="lsrB"
                     /locus_tag="OO309_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
BASE COUNT         1606 a         1765 c         1826 g         1762 t
ORIGIN      
        1 ttgagatgtc cgtctctgac taaatagaca tgtccgcgct acagtctcac cacagcgatc
       61 actgctttca aaggatggga ctcatgacgg catacggaac ggagtaatcg gcaactgatg
      121 ccgggcctcg ctgagcgtgc actgcttatt atccgtgaac gtgccgccaa gaaccgcggt
      181 ttgtgccgga cgcacaacgc ctaacccaag gcaaccaagc tgcacgtcgc ctcctccaac
      241 gaccaccaga gtgcccgcct tcagaccgca gagttccgcc gcttgtgaac ttaccacgcc
      301 cagcaatgtg ccggtttctt tgacaggaga aagaatatcg gcacgcaggc cagccatatc
      361 cagcaatgca ggtttccagg cacgggtggt tagatcaaga agtcccgtgg tgccagcgtt
      421 agagggatcc accgccagtt cgccgctgag catataggcc agccagtcgc tgatcatggt
      481 gatggttgat gcctgacggt aaatatcgga acgatggtgc gccagccaaa gtaatctggg
      541 gatggcactt aaagccagtg tttgtccggt cgctcgataa acttcgtttt caaaggtatt
      601 gttgtgcagt tctttaagtt cgctaacttc gcgtgccgct ctggcatcca cattggcgca
      661 ggcccagatc ggtgttcctt cattgttata taaaacaatg ccttcacgca tagaacatgc
      721 cgaaacggca gcgatatact ccgggtctat gccggcgttg tgcagcgcct ggcgcataca
      781 ctcacacgcc agttgccagt ttttgttgag atcaaattcc atagaaccag gaacgtccgg
      841 tactgccaga tgccgccact ccgcctgtcc cactgctatt tgattgcctt ccaggtcgaa
      901 tatcacagcc cgaatacttc cggtgcctgc atccagcgcc atcaggtagt actttgattc
      961 tgaaggggta aagagtcgag ccattagatt atcctcggct tatgcgttct gtctgttcct
     1021 ctatacgttc tccatcattc ccggtaataa ggtcatgcaa atttaactac gtaaaatcgc
     1081 cgctgctgtg tcctgatcgg taaccagtgc gttgatataa ccgcctttca ttgcagcggc
     1141 aattgcttca gctttatttt ctccccctgc cacgccaacc cggacgggta tggtcttcag
     1201 cgcgcttaaa ggtaagccaa tcagttcgtt atgtattttg atatccgtga caacgtcacc
     1261 ttttgcatca aaaaagtagc ctaaaatgtc gccaaccgcc ccttttcggc caatcattaa
     1321 ctgttcgccc tggctgatat aaccggagcg aatgattgtc gcgtcgtctt gttgactcac
     1381 ggctccaatg ccgacaatcg ccacatccgc tgcttgcgcg gctagcagaa catctttgac
     1441 gcaattttca ttttttagcg tacgggcaat gtcagcggag gatgcccgca acggagccgg
     1501 aataatattc acactgcacg ccgcgttaag ctgcccgata cccgtcatat aagaaccgac
     1561 gccaccggag agcgtgacca ggcgaatttg ctgtgacgaa ataaaaccac ttaagcgttg
     1621 cagcgtattc atggttgcct cgccaaaacc aatcgccagc atctgttgtg gttgaagtaa
     1681 actcatcaac atatgcgccg cgcctatccc tagtcgcccg ccgacatcag catccgcaag
     1741 cccagggatc acccggacat gttgcagcga aaactgacga cgtaattgag tttcatattc
     1801 cagacagcct tcaaagcgag aattaatctg tacgcgaata atgccggact gatgcccttt
     1861 ctccagcaag cgcgacactt tcaaacgggt caggccgaga cgatcgctga tctcgctctg
     1921 ggtcagcccg tcgtgatagt aaaaccacgc gatccgcgcg acctgttctt cttcacacat
     1981 tccctgttct gaaattgccg aatcgttgat tgtcataatt cattcttcac tttgaacata
     2041 tttaaatctt taatgcaatt gttcagttct tgcgcattta tatctgtgat ggcaaccaca
     2101 gtttgactct acgagcatga acaaacgcaa ccgtgaaaat caaaatagca taaattgtga
     2161 tctattcgtc ggaaatatgt gcaatgtcca cctaaggtta tgaacaaatt aaaagtagaa
     2221 atacatttgt tcaaaactca cctgcaaaac tgaacggggg aaatatgcaa acgagtgata
     2281 cccgcgcgtt accgctactt tgcgcccgct cggtttataa acagtattca ggggtcaatg
     2341 tcctgaaagg catcgatttt acgttgcatc agggggaggt ccacgccctg ctcggcggca
     2401 atggtgccgg taaatcgacg ttaatgaaga ttattgccgg tattacccct gctgatagcg
     2461 gtacgctgga gattgggggc aacaactacg ccagattaac gccagttcat gctcatcagc
     2521 tgggcattta tctcgttccc caggaaccgc tgcttttccc aagcctgtcg ataaaagaaa
     2581 acatcctgtt tgggctggca aaaaaacagc tctccatgca gaaaatgaag aacttgctgg
     2641 cggcgctggg ctgccagttt gatctgcata gtctggcagg atcgctggat gtcgccgatc
     2701 gccaaatggt ggaaatcctc cgcgggctga tgcgcgactc gcggattctg atcctcgatg
     2761 aacctaccgc ctcgcttacc cctgcggaaa ccgaacgctt gtttagtcgc ttgcaagagc
     2821 tgcttgctac tggcgtgggt attgttttta tctcgcataa gctgccggaa attcgccaga
     2881 ttgccgatcg aattagcgtg atgcgcgacg gaaccatcgc cttaagcggc aaaaccagcg
     2941 aactgtctac cgacgacatt attcaggcca tcaccccagc ggtacgggaa aaatcgctct
     3001 ctgccagcca aaaattatgg ctggagttac ctggtaaccg cccacaacat gccgtcggaa
     3061 cgccggtgct gacactggaa aatctgactg gcgaaggctt caggaatgtc agcctgacgc
     3121 tcaatgccgg agaaattctg ggcctggctg ggctggtggg agccggacgc acagaactgg
     3181 ccgagacgct ctatggtctg cgtactttgc gtggcggacg cattatgctg aatggtaaag
     3241 agatcaataa attatccact ggagaacgtt tactgcgcgg tctggtttat ctgccggaag
     3301 atcgccagtc atccggactg aatctcgatg cttcgctggc ctggaacgtc tgcgccctta
     3361 ctcataacct tcgtggattc tgggcgaaaa ccgcgaaaga taatgccacc ctggaacgtt
     3421 atcgtcgggc gctgaatatt aaatttaacc aaccggaaca agctgcacgg acattatccg
     3481 gtagcaacca gcaaaaaatc ctcattgcca aatgcctgga agcttcgccg caagcattga
     3541 ttgtcgatga gccgacgcgc ggcgtggatg tctcggcccg taatgatatc taccagctgt
     3601 tgcgcagcat cgccgcacag aatgtggctg tgctgtttat ctcctctgat atggaagaga
     3661 tcgaactgat ggcagatcgt gtgtatgtga tgcatcaggg cgaaattacc cactctgcac
     3721 tgaccgggcg cgatattaat gtcgagacta ttatgcgcgt tgccttcggc gatagtcagc
     3781 gtcaggaggc atcatgctga agtttattca gaacaaccgt gaaatcacgg cattgctggc
     3841 ggtggtgctg ctgtttgtat tacccggttt tctcgaccgc cagtatttaa gtgtgcaaac
     3901 gctgaccatg gtttatagca gcgcgcaaat cctgatcctg ctggcaatgg gcgcgacgct
     3961 ggtaatgctt acgcgcaata ttgatgtttc agtgggttcg attaccggaa tgtgcgcggt
     4021 gctgttgggg atgttactga acgcaggata ttcactacct gttgcctgtg tcgcgacttt
     4081 actgcttggt ttgctcgcgg gatttttcaa cggtgccctg gtcgcgtggc taaagatccc
     4141 tgccattgtt gccacccttg gcacgttagg gttgtacaga ggcatcatgt tgctgtggac
     4201 tggcggtaaa tggattgaag ggttacccgc cgaactgaaa cagctctccg ccccgctgct
     4261 gcttggcatt tcagcaattg gttggttgac gataattctg gtggcattta tggcctggct
     4321 gctggcgaag acggcgtttg gacgcagttt ttatgccacg ggcgataatt tacagggcgc
     4381 tcgtcaactg ggcgttcgta cggaagccat tcgcattgtg gcattttcgt tgaacggctg
     4441 catggcggca ctggcgggaa ttgtgtttgc ttcgcagatt ggttttatcc tcaaccagac
     4501 cggtaccggg ctggagatga aagcaattgc agcctgcgtg ctgggcggca ttagtttgct
     4561 cggtggttcc ggtgcgatca tcggtgcggt actcggcgca tggttcctga cgcagatcga
     4621 tagcgtactg gtgctgttgc gcattccggc atggtggaat gattttatcg cgggtctggt
     4681 tctgctggcg gtgctggtgt ttgatggacg cctgcgttgt gcgctggaac gtaatctacg
     4741 gcggcaaaaa tatgcccgct ttatgacgcc accgccatcc gttaaacccg cttcgtcagg
     4801 taaaaaacgg gaggccgcat aatgcgtatt cgctacggtt gggaactggc tcttgccgca
     4861 ctgctcgtta ttgagattgt cgcatttggt gcaattaacc cgcgaatgtt agatctcaat
     4921 atgttgctgt tcagcaccag tgactttatc tgcattggca ttgtcgctct accgctgacg
     4981 atggtgattg tcagtggcgg gatcgatatt tcgtttggtt cgaccatcgg cctctgcgcc
     5041 attgcattgg gcgtactgtt tcaaagtggt gtgccgatgc cgctggcgat actcctgacc
     5101 ttactgctcg gcgcattgtg cgggctgatc aacgccggat taattatcta taccaaagtt
     5161 aacccgctgg tgattacgct tggcacgctg tatctgtttg ccggaagcgc tctgctgctt
     5221 tccggtatgg ccggagcgac ggggtacgaa ggtattggtg gattcccgat ggcgtttaca
     5281 gatttcgcta acctggatgt gctgggactc cccgttccgc tgattatctt cctgatatgt
     5341 ctcctcgttt tctggctctg gctgcataaa acccatgccg gacgtaatgt gtttttgatt
     5401 gggcaaagcc cgcgcgtggc gctttatagc gcgattccag ttaaccgcac cttatgtgcg
     5461 ctctatgcca tgacggggct ggcgtctgcg gtcgccgctg tgctgctggt atcgtatttt
     5521 ggttcagcac gttccgatct cggtgcgtcg tttctgatgc ccgccatcac cgccgtggtg
     5581 cttggcgggg ccaatattta tggtggttcc ggttccatta tcggcaccgc cattgcggtt
     5641 ttattagtgg gatatttgca gcaaggtttg caaatggcag gagtgccaaa tcaggtgtcc
     5701 agcgcccttt ccggtgcgct gcttatcgtc gttgtcgtag gtcgttccgt tagcctgcat
     5761 cgccagcaaa ttaaagagtg gctggcgcgt cgggccaata acccattgcc ataaaggata
     5821 tcttcatgac acttcatcgc tttaagaaaa tcgccttact tagcgctctt ggcattgccg
     5881 caatctctat gaatgtgcag gccgcagagc gtattgcatt tattcccaaa ctggttggcg
     5941 tgggattttt taccagcggt ggcaacggcg cacaacaagc gggtaaagag ctgagcgttg
     6001 atgtgaccta cgacgggccg acagaaccca gtgtttctgg tcaggtacag ttgattaata
     6061 acttcgtcaa tcaaggttat aacgccatta ttcgtttctg cggtttcgcc tgatggcttg
     6121 tgtccggcac tgaaacgcgc catgcaacgt ggtgtgagag tgctgacctg ggactctgat
     6181 actaaaccgg agtgccgctc ttactacatt aatcagggaa cgcccgccca gttgggaggt
     6241 atgttggtgg atatggcggc gcgtcaggtg aataaagaca aagccaaagt cgcgtttttc
     6301 tactcaagcc ccaccgttac ggaccaaaac cagtgggtga aagaagcgaa agcgaaaatc
     6361 gccaaagagc atccaggctg ggaaattgtc actacgcagt ttggctataa cgatgccact
     6421 aaatcgttac aaaccgcaga aggaatatta aaagcgtata gcgatctcga cgccattatc
     6481 gcccccgatg ccaacgccct gcccgctgcc gcacaagccg cagaaaacct gaaaaatgac
     6541 aaagtagcga ttgtcggatt cagtacgcca aatgtgatgc gcccgtatgt agagcgcggc
     6601 acggtgaaag aatttggact gtgggatgtg gttcagcaag gcaaaatttc agtgtatgtc
     6661 gcggatgtat tattgaaaaa aggatcaatg aaaacgggcg acaagctgga tatccagggc
     6721 gtaggtcagg ttgaagtctc gccaaacagc gttcagggct atgactacga agcggatggt
     6781 aatggcatcg tactgttacc ggagcgcgtg atattcaaca aagagaatat cggcaaatac
     6841 gatttctgat gtgcattact taaccggagt aagttatggc agatttagac gatattaaag
     6901 atggtaaaga ttttcgtacc gatcaaccgc aacaaaatat cccttttacc ctgaaaggt
//
LOCUS       DAJTQQ010000157         6682 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000157 DAJTQQ010000000
VERSION     DAJTQQ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.158"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..1407
                     /gene="sbcB"
                     /locus_tag="OO309_003829"
     CDS             <1..1407
                     /gene="sbcB"
                     /locus_tag="OO309_003829"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR5848790.1"
                     /translation="QPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGEPEVFYC
                     KPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYNNVRFDD
                     EVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGLPSFRLE
                     HLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQ
                     MKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDIDTLRERL
                     YTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLKILRENP
                     QVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFV
                     DKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLVQQYADD
                     KEKVALLKALWQYAEEIV"
     gene            complement(1450..1677)
                     /locus_tag="OO309_003830"
     CDS             complement(1450..1677)
                     /locus_tag="OO309_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR5848791.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1691..2749)
                     /gene="yeeE"
                     /locus_tag="OO309_003831"
     CDS             complement(1691..2749)
                     /gene="yeeE"
                     /locus_tag="OO309_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR5848792.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(2928..4286)
                     /gene="plaP"
                     /locus_tag="OO309_003832"
     CDS             complement(2928..4286)
                     /gene="plaP"
                     /locus_tag="OO309_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR5848793.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(4276..4338)
                     /gene="yoeI"
                     /locus_tag="OO309_003833"
     CDS             complement(4276..4338)
                     /gene="yoeI"
                     /locus_tag="OO309_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR5848794.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(4499..5482)
                     /locus_tag="OO309_003834"
     CDS             complement(4499..5482)
                     /locus_tag="OO309_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5848795.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(5528..6352)
                     /locus_tag="OO309_003835"
     CDS             complement(5528..6352)
                     /locus_tag="OO309_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5848796.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(6435..6680)
                     /gene="yoeB"
                     /locus_tag="OO309_003836"
     CDS             complement(6435..6680)
                     /gene="yoeB"
                     /locus_tag="OO309_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003850603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR5848797.1"
                     /translation="MWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEP
                     LKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1794 a         1762 c         1571 g         1555 t
ORIGIN      
        1 caacctacct tcctctttca cgattacgaa acctttggca cgcaccccgc gttagatcgc
       61 cctgcacagt tcgcagccat tcgcaccgat gacgaattca atgtcatcgg cgaacccgaa
      121 gtcttttact gcaagcccgc ggatgactat ttaccccggc ctggagcagt attaattacc
      181 ggtattaccc cgcaggaggc acgggcgaaa ggagaaaacg aagccgcgtt tgccgcccgt
      241 attcactcgc tttttaccgt accgaagacc tgtattctgg gctacaacaa tgtgcgtttc
      301 gacgacgaag tcacacgcaa cgttttttat cgtaatttct acgatcctta cgcctggagc
      361 tggcagcatg ataactcgcg ctgggattta ctggatgtta tgcgtgcctg ttatgccctg
      421 cgcccggaag gaataaactg gcctgaaaat gatgacggtc taccgagctt tcgccttgag
      481 catttaacca aagcgaatgg tattgaacat agcaacgccc acgatgcgat ggctgatgtg
      541 tacgccacta ttgcgatggc gaaactggta aaaacgcgtc agccacgcct gtttgattat
      601 ctctttaccc atcgtaataa acacaaactg atggcgttga ttgatgttcc gcagatgaaa
      661 cccctggctc acgtttccgg aatgtttggg gcatggcgtg gcaataccag ctgggtggca
      721 ccgctggcgt ggcatcctga aaatcgcaat gccgtaatta tggcggattt ggcaggagac
      781 atttcgccat tactggagct ggatatcgac acattgcgcg agcgtttata taccgcaaaa
      841 gccgatcttg gcgataacgc cgccgttccg gttaagctgg tgcatatcaa taaatgtccg
      901 gtactggccc aggcgaatac gctacgcccg gaagatgccg accgactggg aattaatcgt
      961 cagcattgcc tcgataacct aaaaattctg cgtgaaaatc cgcaagtgcg tgaaaaagtg
     1021 gtagcgatat tcgcggaagc cgaaccgttt acaccttcag ataatgtgga tgcacagctt
     1081 tataatggct ttttcagtga cgccgatcgt gcagcaatga aaattgtgct ggaaaccgag
     1141 ccgcgtaatt taccggcact ggatatcact tttgttgata aacgaattga aaagttgttg
     1201 ttcaattatc gggcacgcaa cttcccgggg acgctggatt atgccgagca gcaacgctgg
     1261 ctggagcacc gccaccaggt cttcacgcca gagtttttgc agggttatgc tgatgaattg
     1321 cagatgctgg tacaacaata tgccgatgac aaagagaaag tggcgctgtt aaaagcactt
     1381 tggcagtacg cggaagagat cgtctaattt gaagccgccg ccgctggtac ggcggaggac
     1441 aatgttgagt caggcttttt gaacggtgat gctccacgct gcatcgccaa tctgctgata
     1501 atcggtgatg gcatgtcctt cttctgccgc ccactgtggg attgcttcag ttgcctgggt
     1561 gcaatcaaac tcaattacca actcatcgcc gctggccatt tctgccagtg ctgcttttgc
     1621 ttctatgagt ggaaacggac agacctgcgt caccacatcc agctttttaa tcgccatctt
     1681 tacaccttac ttaatttgcc gcagcagttg ccagtcgcgc cttacgctgc ggtcgaacat
     1741 acacaagcca ggacgcagtc cagactccga gaatcataaa tacaaggcca atccagccct
     1801 gccaggtcat cattgcagtc ataaccagtc cattaccgat agagcaacca cctgcaatac
     1861 tggcaccgaa gcccatcagt acaccaccta acccgctacg taatgttgtt tgtgcatcag
     1921 ctgcgcgaac gcggaactca cggctcgctt tggctgcaat aaatgcctcc acgaagatcc
     1981 ctaacaccag gaaaacgccc cagttaatgt atttcacgtc acccgcgacc agaaattgaa
     2041 gaatattggc cgttggggaa gtgactccta acccaaacat gcgtccggtt gcttcactca
     2101 ggggccaggc taaaagcgca atcaaaccga tgagtacagc agtgacaaag ggatgccagc
     2161 gtttttcaaa cagaatatga gcgatcccgg ttcggcgcgg cggtaaggtc gcgactttga
     2221 gttttggctt cttcaactct ttcatcacca cccagagcgt tatcaccagc aaaacggtaa
     2281 ccaacggcca cacagacaaa ttaaaagtct cagcaataga gttatgttca gtagtgtagt
     2341 gctgcaaggt ttgatttaaa ccactggcgt gtggagaacg catcaccgca ctcatcacca
     2401 tataagtgaa aagcgcgatc caactgccga tcaatccctc acccgcgcga taccaggtcc
     2461 cggtggcaca tccacccgcc agaacaattc ccagcccgaa aagataccca cctataacag
     2521 taccaagcca cgggaacgcc ccggcttcgt aagtcagtag gcccgcctga attaacgcaa
     2581 aaacccccac gctttgtgcc gaaattgcaa tcagcaaggc gtaaaacatg cgattatttt
     2641 tcgcgatata catatcgcga aaaccaccag tcaggcaaaa acgcccacgc tgcatgacaa
     2701 atcccagcag agcaccacaa attagcccgc ttaatatcat tgaaaacata aattaattaa
     2761 ccagatgaat gttaatgggg aaatttttca tgactggtgg aatgcagacc aataaccaaa
     2821 ttctctaaat tagaacaaat ggttattaat gaggatttcg ataaaaaaga ataaaaaaac
     2881 cggagccaaa tgttcattcg actccggttt attattagaa aagatgatta tgctacgtct
     2941 tcgtactgcg gaaccggatt acggaagctc ttggtgacgc aagccaggta aatcagaccg
     3001 atagcagccc agatcagacc cagaaccatt gagctttctt ccaggttaac ccacagcgca
     3061 ccaacggtca gcgcaccaca catcggcagg aacagatact ggaagtgatc tttcagcgtc
     3121 ttgttacgct tctcacggat ccagaactgc gagatgaccg acaggttaac aaaggtgaac
     3181 gccaccagcg caccaaagtt aatcagcgcc gttgccatta ccaggtcgaa gttgattgcc
     3241 agcagggcaa tcgcgccaac caggatgatg ttcatcgccg gagtacgcca tttcgggtgt
     3301 acataaccga agaagctttt cgggaacacg ccgtcacgac ccattacgta catcagacgc
     3361 gctacgcctg cgtgcgccgc cataccggac gccagtacgg taatggtgga gaagatcagc
     3421 gcgccaacct ggaacgcttt acccgcaacg tacagcatga tttcaggctg tgacgcatcc
     3481 ggatctttaa agcgagagat atccgggaag tacagctgca ggaagtaagt tgcaaagatg
     3541 aagatcatgc cgccaatcag cgcggtcagg aaaatcgcac gcgggatcac gcgctctgca
     3601 tctttggttt cttccgacag attgctgatg ccgtcaaagc cggtaaagga gaagcacagg
     3661 attgtcgccc cggtaatcat cgggataaca tgcgcatcgc cagaccagaa cggacgcgtg
     3721 ctcgccagcg taccagcgcc ttcaccttca aatacgccat aaacaaccat gcccagaatc
     3781 accgcgatca gcactacctg caacacgacg attacagtgt tgacgttcgc tacggatttc
     3841 agactacgca ggttaaaggc ggtcataaag gccaccagcg ccaccacaaa catccacgat
     3901 ggaatggaag gcaccagagc ttcaaaatag attttcgcca gcagaatgtt gatcatcggc
     3961 gcgaacagat agtcgagcag agaagaccaa cccaccataa agccgacagt cgggctaatg
     4021 gatttctggg cgtaagtgta tgcagagcca gcagaaggat agcggcgaac cagcttcccg
     4081 tagctcagag ccgtaaacag gatcgcaatc aatgcgaacg cataggctgt cggcacatga
     4141 ccatccgtga ggcctgaaac gataccaaat gtatcaaaca gcgtcatcgg ctgcatatag
     4201 gctagaccca tcattacaac cggaactaac gtaagcgttt tacgcaattc cacgcgagag
     4261 gtgtttggag taacgttatg cgacatggtt attctccttt acggtgataa ccgtcgcgta
     4321 agcaaaaaat tgccccattt ttttggattc ctcagcgaca acaactgtcg atttttagta
     4381 aatatctatc cggtacgaag cccggcctct tggtatgaat gattggtttg aagcaaaaaa
     4441 taaccgacgc taattaaacg tcggttttta atcatttttt gacagcggcg cattgtgcct
     4501 aaatgggggg gaaatgacaa gagaatgaga ggcttgtcat aataattttt ctttgaatgg
     4561 ctgattttcc gtcatcagat tttgcataaa caccggcgat cgcactattt gctaaaattt
     4621 catctcgcca ccagccgctg gcaagccctg cggtctgttc attccagcca atccagacag
     4681 gttcaaagag agtttgggcg accactttct tctcgattaa cgcgccacta tcgagaaaac
     4741 gttgcgccag ataccgtggt aaatagccgc agccaaggcc gctaatttgc agctccagtt
     4801 tggttttaaa atcgaaaacg gtaatggctt cttgttcgtc gagaagttgt gaggccgtag
     4861 aagcgcctgc ctgcgcagta tcccccacca caatcgcccg atatcgttta atgatacgac
     4921 ggtttaacgg ttcttcttcc tgggctaacg gatgatgagg cgcgaccgca aagacttgtt
     4981 caagatcgcc caaccgcgag aaaccaaact cactggaaga tggtggctca tgcatcgcgc
     5041 caacgataat atccgctcgc ccctgagtca gggcatccca ggaaccaccg agtacgccat
     5101 tgataaactt caggcgcgtc acgctatgat gttgataaaa agcttcaatg agaggcgcaa
     5161 gcagagaaaa aggaaacgtg tcgtccacgc caatcaccag ctcgttttcc cagccttcat
     5221 gcagtttgat cgcctgcttt tccagttctc gcacggtatg cagaacttcc cgccctttct
     5281 ctaataacat ttttccggtg cgggtgaatt tagcgcggtg tccgctacga tcaagcaatt
     5341 gaatatttag gtcgctttcc agcttgtgaa cggtgtaact cagagcggac ggcgtcttgt
     5401 agagtttggc tgacgccgcc gcaaaactgc cctctttttc tatggcatcg aggatcatca
     5461 acacatccag cagtggtttc atacacgccc ccttgcagtg catgcggcgt cctgctggcg
     5521 tgatcgttta ctccaacggc attaccagcg gatcgggata ctggtattca aatcccagtt
     5581 cattacaaat ccgactgcca tcaataatct tacctttgcc gctgtccaga ctatttctga
     5641 actgcggagg ttgcagcccc agtagacggg ccatctgcgg atagaaaaca ttacgcgcag
     5701 ggtgagctgg cgcacatata ttatagatgt gtccgccttt aggtgcctgt aacaacagag
     5761 tgatagcgcc aataacatct tctaaatgga ctaaattaac accatgttca ccatcagggg
     5821 cggtttttcc ggcaaagaag cgtcccggat gacgtcccgg tccgaccagg cccgcaagac
     5881 gcagaatatc gaccgaagta ccgggtaaat tgtgcagcca gtcttcgagt tcttctaata
     5941 ctcgtccact gttggttacc ggattacgcg gggtagtttc tttaaccgtg ccttgcgcgt
     6001 cgccatagac agatgttgag ctggtaaaaa taattcgggg aatacgatgg gccagcgcgc
     6061 tatccactaa ctcttgtacc gcttgtaaat agaactcatc gccggggccg ctacgacgtg
     6121 ccggaagcgt aatgaccagc gcatcggcat ccataagggc atccagatcg tcagaatcgc
     6181 aaactaactc aggctccatg cgaagcagat agctatcaat gccactcatt cgggccgctt
     6241 cgacaccatc ttgtgtggtt ttactcccgg tgacttgcca gcctcgcgct gaaagtgaca
     6301 tcgccagcgg catacctaac caccctaacc cgacaattgc gacctttttc atccgttatc
     6361 tcctgataca ccttagatct ataaggctac gctagcgtat caaaactgac aattcattct
     6421 atgaatgaat ctgttcaata atgataacga catgctgcaa tgagcagtga atcatcagta
     6481 accgcgtata ccagacggtg ctcctctgta atgcgtcggg accagaaacc tgacaaatta
     6541 tgtttcaggg gttctggttt ccccttacct tcaaatggtg ttctgcgggt atctttgata
     6601 agttcattga tctttttaac aattcgctta tctgtttcct gccagtacag ataatcatcc
     6661 catgattcct cagaccagat ta
//
LOCUS       DAJTQQ010000158         6579 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000158 DAJTQQ010000000
VERSION     DAJTQQ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6579
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.159"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            34..2466
                     /locus_tag="OO309_003837"
     CDS             34..2466
                     /locus_tag="OO309_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR5848798.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2533..4002
                     /locus_tag="OO309_003838"
     CDS             2533..4002
                     /locus_tag="OO309_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR5848799.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4002..5663
                     /locus_tag="OO309_003839"
     CDS             4002..5663
                     /locus_tag="OO309_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR5848800.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            5884..6225
                     /gene="symE"
                     /locus_tag="OO309_003840"
     CDS             5884..6225
                     /gene="symE"
                     /locus_tag="OO309_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCR5848801.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6272..>6579)
                     /locus_tag="OO309_003841"
     CDS             complement(6272..>6579)
                     /locus_tag="OO309_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848802.1"
                     /translation="LRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLN
                     MQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
BASE COUNT         1936 a         1576 c         1519 g         1548 t
ORIGIN      
        1 tcagaatcgt cctgataaaa gcgagaaccc cccatggcgg aactgaacct aagtaacctg
       61 acggaagcag acatcattac caaatgcgtt atgccagcca ttctcaatgc aggctgggac
      121 aacacaacgc aaatcagaca ggaggtcaaa ctccgggacg gtaaagtcat cgtacgtggt
      181 aaagttgcgg cacgcagaac ggtaaaatct gctgacatcg tgctgtacca caagccaggc
      241 attcccttag ccgtgattga agcaaaagcc aacaaacatg aaattggcaa agggatgcaa
      301 cagggcattg aatatgcgcg cctgctggac gttccttttg tttttgccac caacggtgat
      361 ggctttatct tccgcgatgc caccgcagcc gaaggtgaat gccttgaaaa gcaaataacg
      421 ctggatgact tcccctcccc cgctgaactc tggcaaaaat tctgcctctg gaaaggatac
      481 acacaagctc agcttccggt gattactcag gactattacg acgatggcag cggcaaatcg
      541 cctcgttatt atcaacttca ggcaatcaac aaaaccattg aagccgtctc caacgggcaa
      601 aaccgcgttc tgctggtcat ggcgaccgga acagggaaaa cctataccgc attccagatc
      661 atctggcgcc tgtggaaatc aaaaaataaa aaacgcattt tgttccttgc cgatcgcaac
      721 attctggtcg accaaaccaa aaataatgat ttccagccat ttggtacggc aatgaccaaa
      781 gtcagcggac gcaccattga tcccgcttat gaaattcacc tcgcgctcta tcaggctata
      841 actggcccgg aggaagacca aaaagcgttt aaacaagtcg caccagattt cttcgatctg
      901 atcgtgatcg acgaatgcca tcgcggcagc gcatccgaag acagcgcctg gcgagagatc
      961 cttgattatt tcagttccgc cacccaaatt ggcttaaccg ccacgccaaa agagacgcat
     1021 gaagtctcca gcacggatta cttcggcgat ccggtttacg tctactcact aaaagaaggg
     1081 atcgaagacg gcttcctcgc tccttataaa gttgtccgtg ttgatattga tgtcgacctg
     1141 caaggctggc gcccaaccaa agggcaaact gacttaaacg gcgaagtgat tgacgatcgt
     1201 atctataacc agaaagattt cgatcgcacg atggtgatcg acgaacgcac agaactggtt
     1261 gccagaacca ttaccgacta cctcaagcgc accaatccga tggataaaac catcgtcttc
     1321 tgtaacgaca tcgatcatgc agaacgtatg cgccgcgccc tggttaatct caacccgcag
     1381 caggtgaaaa agaacgacaa atacgtcatg aaaatcaccg gcgatgatga aattggcaaa
     1441 gctcagttgg ataacttcat caacccgaaa aaaccgtacc cggttatcgc gaccacttca
     1501 gagctgatga ccactggtgt ggatgctaaa acctgcaaac tggtagtact agaccagaac
     1561 atccagtcga tgaccaaatt caagcaaatt atcggccgtg gtacacgcat cgacgaacgt
     1621 tacggcaaac tctggtttac catcctcgac tttaaaaaag ccaccgaact gtttgccgat
     1681 gagcgtttcg atggcattcc cgaaaaagtc atggatacca caccagagga tatcgccgat
     1741 ccagaatctg attttgaaga gaaactcgaa gaaatcagcg aacatgacga cgaacaggta
     1801 acaggcgttg atgaaccgcc tgcgccacca taccaggtta ccgataccga tgatgtcggc
     1861 ccatttccgg aagaagacga gaagaaaatc cgcaagtttc acgtcaacgg tgtagcggtg
     1921 ggcgttattg cacagcgcgt tcagtattac gacgccgacg gtaaactggt taccgaatcc
     1981 tttaaagatt acacccgcaa aacactgctc aaagaatatg cctcgctgga tgactttacc
     2041 cgcaagtggc aggacgccga tcgcaaagaa gcgatcattc acgagctgga gcaacagggg
     2101 atcatctggg aagtactggc agaagaagtc ggtaaagatc tcgacccgtt cgacatgctt
     2161 tgccacgtag tgtatggtca gccgccgtta acccgcaaag agcgcgccga gaacgtgcac
     2221 aagcggaact acttcacaaa atactctgaa gcagcgcaag ccgtgctcga taatctgctg
     2281 gataaatacg ccgatgcggg cgtacaggag atcgaaagta ttcaggtgct gaaacttaaa
     2341 ccattcgaca gcatgggcac cttaccggag attattaaaa ccggatttgg cgaccgtaac
     2401 gggtataatc aggcgctcag cgagctggaa aacgaaatct accaattacc gccccgctct
     2461 gcttaacaac tctcttttgc gccttctgca accatcatgt tgcagaaggc cttgtttttc
     2521 atggaataca acatgtctat cagctcagta atcaaatcat tacaagatat tatgcgcaaa
     2581 gatgccggtg tggacggcga tgcgcagcgt ctcggtcagc tctcctggct gctgtttttg
     2641 aaaatcttcg atgcccagga agaggcgctg gaactggagc aggataacta tcaatatccg
     2701 atcccacagc gttatttgtg gcgcagttgg gccgccaacg cgcagggcat taccggtgat
     2761 tccctgctgg aattcgttaa tgatgatctg tttccggcgt tgaaaaacct cactgcgcct
     2821 atcgataaaa acccacgcgg ctacgtggtg aagcaggcgt tcagcgatgc ctataactat
     2881 atgaaaaacg gtacgctact gcgccaggtg atcaacaagc tgaacgaaat tgactttacc
     2941 agcgccagcg aacgccatct gtttggtgat atttacgaac agatccttaa agatctgcaa
     3001 tctgcgggca atgcgggcga attctatact ccacgcgccg tcactcgctt tatggtggat
     3061 cgcgttgatc cgaaactcgg cgaatccatt atggacccgg cctgcggtac gggcggtttt
     3121 cttgcctgcg cgttcgatca tgtaaagaac aaatacgtga agagcgtcgc cgatcatcag
     3181 acactgcaac aacagatcca cggtgttgag aaaaaacagc ttccgcacct gctggcgacc
     3241 accaatatgc tgctgcacgg cattgaagta ccggtacaaa ttcgtcacga caacaccctg
     3301 aataaaccgc tttcctcctg ggatgagcaa ctggatgtca ttgttaccaa cccgccgttt
     3361 ggtggcacgg aagaagacgg tattgagaag aactttccgg cagagatgca aacccgcgaa
     3421 acggcggatc tgtttctgca actgattgtg gaagtactgg cgaaaaacgg tcgtgcggcg
     3481 gtggtattgc cggatggcac actatttggc gaaggtgtta aaaccaaaat caaaaagcta
     3541 cttaccgaag agtgcaacct gcataccatc gtgcgtttac cgaatggtgt gtttaatccc
     3601 tataccggca ttaaaaccaa cctgttgttc tttaccaaag gtcagccaac caaagagatt
     3661 tggttctatg aacatccgta tccggcgggc gtgaaaaact acagcaaaac caagccgatg
     3721 aagtttgaag agtttcaggc ggagatcgac tggtggggta acgaggccga tggttttgcc
     3781 agccgcgtcg agaatgagca agcgtggaaa gtcagcattg atgacgtgat tgcgcgcaac
     3841 ttcaatctgg atattaaaaa cccacatcag gcggaaaccg tcagccatga tccggacgaa
     3901 ctgttagcgc agtatgcaaa acagcaggcg gagatccaga cgctgcgtaa tcaactgcgc
     3961 gatattcttg gcgctgcgct gtctggcaag gaggttaact aatgagtgtg gaaaagctga
     4021 tcgttgatca tatggaaacc tggacctcgg cgttgcaaac ccgttccacc gccgggcgcg
     4081 gcagctccgg taagattgat ttgtatggta ttaagaaatt gcgtgagctg attctggaac
     4141 tggcggtgcg cggtaaactg gtgccgcagg atccgaatga tgaaccggcg tcggacctgc
     4201 tgaagcgtat tgcggcggaa aaagcagagc tggtgaagca aggcaaaatt aaaaagcaaa
     4261 aaccactgcc agaaattagt gaggaagaga agccgtttga attgccggag ggatgggagt
     4321 gggtgcacct ccctgatatt tattgctcaa tttctgagtc atctagaaaa ataaaatcct
     4381 ccgaaatact acctgaggga aaatatcctg ttatcgagca aagccaagaa tttatttctg
     4441 gttattgcaa caatgaatgt ctgttaataa agttaaataa tccagttata gtttttggtg
     4501 atcacacaag gaatattaaa tttattgatt ttgattttgt tgtcggcgct gacggtgtga
     4561 agattttatc acctatatta atttgtgagc gtttcttttt ttggcagtta cgatcattta
     4621 aattagatgt aagaggatac gcgagacatt ttaaagtatt aaatagctgc ttatttgcat
     4681 taccccccat tgcagaacaa gagcgaatag ttgaaaaagt tagctctttg atgtccctct
     4741 gcgaccaact agaacagcaa tccctgacca gtctggacgc acatcagcaa ctggttgaaa
     4801 ccctgttggg aacgcttaca gacagccaaa acaccgcgga actggctgaa aactgggcgc
     4861 gtattagcga gcatttcgac accctattta ccaccgaagc cagcgtggat gcgttaaaac
     4921 agaccattct gcaactggcc gtaatgggca aacttgtgcc gcaggatccg aatgacgaac
     4981 ccgcctctga actgctcaaa cgtattgcgc aggaaaaagc tcaattggtg aaagaaggga
     5041 aaataaaaaa acaaaaacca ctgccgccaa ttagcgatga ggaaaaaccg tttgaacttc
     5101 cggaagggtg ggaatggtgt cgtattggta atatagttaa tattaaatct gaattagtat
     5161 caccaaaaga ttatttaaat ttatatcaag tagcacctga tattattgaa aagggcactg
     5221 ggagagtgat atcaaagaga acagtgaaag aatcaggagt caaagggcca aatagtcgct
     5281 tttataaagg acaaattgtt tattctaaaa tccgcccgtc attatccaaa gtttttttag
     5341 cagagtataa tggtctttgc agtgctgata tgtatccatt agactgttac attaatccaa
     5401 attacttatt gaaatatatc ctttccattc catttcttat gcaagtcaaa aaagcagaga
     5461 acagaataaa aatgccaaaa cttaattctg acagttttta caatattatt gttgcgattc
     5521 ccccctataa tgaacaacaa gcaatatttg ataagataaa ttctattgaa gcagtatgca
     5581 atggtcttat ttcttatatt gggatttatc ataaaaccca actgcatctt gcagacgcac
     5641 tcactgacgc agcgataaac taattccctc gccctcacaa aaccaataaa gcggctaaat
     5701 agccgcttta ttcacatcac caaaatttat atttcacgct aattttcatc ttattttcat
     5761 taatttttag ctggactttc cctgtattta ctgatgatgt atccaggtat ttagcacggt
     5821 gcggatgtgc gccaacacac tcgcaccgct aatcacaatc actatttata gagaatagta
     5881 atcatgactg acacgcattc tattgcacaa ccgttagaag cagaagtctc cccggcaaat
     5941 aatcgtcaat taaccgtgag ttatgcgagt cgttacccgg attacagccg tattcccgcc
     6001 atcaccctga aaggtcagtg gctggaagcc gccggttttg ccaccggcac ggcggtagat
     6061 gtcaaagtga tggaaggctg tattgtcctc accgcccaac ccgccgccgc cgaagagagc
     6121 gaactgatgc agtcactacg caaggtgtgt aagctgtcgg cgcgtaaaca aaggcaggta
     6181 caggaattta ttggggtaat tacaggtaaa cagaaagtcg cctgatacat tgacccggta
     6241 gcgccagtgc taccgggtat tattcgccgc gttataccaa cgcgctctcc atctgttttt
     6301 caaatgcctg acgaattaat cccataatgt aaggaagttg tgcgaggtcg ctgaaaccaa
     6361 tttctacatc gccattaccc cagcgcccaa catttgtcac atctttggca atacctttcg
     6421 gatcgaccag ttcatgaaac tgcatattaa gcgacaatcg cagtcggctt ttttgcggca
     6481 caacatcaac aaaattcgtt tccgctttaa acgcaatata tagcttcaat acttcctgcg
     6541 tgatccctgc gtccaggcgc ataacttcat cgcgaagat
//
LOCUS       DAJTQQ010000159         6573 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000159 DAJTQQ010000000
VERSION     DAJTQQ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.160"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..1091
                     /locus_tag="OO309_003842"
     CDS             <1..1091
                     /locus_tag="OO309_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HCR5848803.1"
                     /translation="QTRDYHDNFTTFSAEEWQPQNRGFDYFMGFHAAGTAYYNSPSLF
                     KNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAYNAPHLPNDNPAPDQYQKQ
                     FNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTIILFTSDNGAVIDGPLPLNG
                     AQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDFYPTALDAADISIPKDLKL
                     DGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWDNYHKFVRHQSDDYPHNPN
                     TEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDNLAAANPQVVKEMQGVVRE
                     FIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1143..2300
                     /locus_tag="OO309_003843"
     CDS             1143..2300
                     /locus_tag="OO309_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5848804.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2266..2388)
                     /locus_tag="OO309_003844"
     CDS             complement(2266..2388)
                     /locus_tag="OO309_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848805.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            2591..4276
                     /locus_tag="OO309_003845"
                     /pseudo
     CDS             2591..4276
                     /locus_tag="OO309_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4314..>6573
                     /locus_tag="OO309_003846"
     CDS             4314..>6573
                     /locus_tag="OO309_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5848806.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADIL"
BASE COUNT         1940 a         1433 c         1388 g         1812 t
ORIGIN      
        1 aacaaacgcg tgactatcat gacaacttca ccacattttc tgcggaagaa tggcaacctc
       61 aaaaccgtgg ctttgattac tttatgggat tccacgctgc aggaacggca tattacaact
      121 ccccttcact gttcaaaaat cgtgaacgtg tccccgcaaa aggttatatc agcgatcagt
      181 taaccgatga ggcaattggc gttgttgatc gtgccaaaac acttgaccag ccttttatgc
      241 tttacctggc ttataatgct ccgcacctac caaatgataa tcctgcaccg gatcaatatc
      301 agaagcaatt taataccggt agtcaaacag cagataacta ctacgcttcc gtttattctg
      361 ttgatcaggg tgtaaaacgc attctcgaac aactgaagaa aaacggacag tatgacaata
      421 caattattct ctttacctcc gataatggtg cggttatcga tggtcctctg ccgctgaacg
      481 gggcgcaaaa aggctataag agtcagacct atcctggcgg tactcacacc ccaatgttta
      541 tgtggtggaa aggaaaactt caacccggta attatgacaa gctgatttcc gcaatggatt
      601 tctacccaac agctcttgat gcagccgata tcagcattcc aaaagacctt aagctggatg
      661 gtgttccctt gctgccctgg ttgcaagata agaaacaagg cgagccacat aaaaatctga
      721 cctggataac ctcttattct cactggtttg atgaggaaaa tattccattc tgggataatt
      781 accacaaatt tgttcgccat cagtcagacg attacccgca taaccccaac actgaggact
      841 taagccaatt ctcttatacg gtgagaaata acgattattc gcttgtctat acagtagaaa
      901 acaatcagtt aggtctctac aaactgacgg atctacagca aaaagataac cttgccgccg
      961 ccaatccgca ggtcgttaaa gagatgcaag gcgtggtaag agagtttatc gacagcagcc
     1021 agccaccgct tagcgaggta aatcaggaga agtttaacaa tatcaagaaa gcactaagcg
     1081 aagcgaaata actaaacctt catgcggcgg atttttccgc cgccttattg agcgagatag
     1141 cgatgcacgt tacagccaag ccctccagtt ttcaatgtaa tctcaaatgt gattactgtt
     1201 tttaccttga aaaagagtcg cagtttactc atgaaaaatg gatggatgac agcactctga
     1261 aagagttcat caaacaatat atcgaagcgt ctggcaatca ggtctatttt acctggcaag
     1321 gcggtgaacc cactctggct ggcctggatt ttttccgtaa agttattcac tatcaacaac
     1381 gctatgcagg ccaaaaacgt atttttaata cattacaaac gaatggcatt ttattgaata
     1441 atgaatggtg tgcctttctc aaagaacatg aatttctggt tggtatctcg atcgatggcc
     1501 cccaggagtt acatgaccgt tacagacgca gtaattcagg taacggtact tttgcaaaag
     1561 tgatagcagc catcgagcgt ctgaaatcat atcaagtaga gtttaatacg ttaaccgtca
     1621 ttaataacgt taatgtccat tatcctcttg aggtttatca ttttttaaaa tctatcggca
     1681 gtaaacatat gcaatttatc gaattgctag aaaccgggac gccgaatatt gatttcagtg
     1741 gtcatagtga gaacacattc cgtatcattg atttttctgt gcctcccacg gcttatggca
     1801 agtttatgtc aaccattttt atgcaatggg ttaaaaacga tgtgggtgaa attttcattc
     1861 gtcagtttga aagctttgtc agccgttttt tggggaatgg gcatacaagt tgtattttcc
     1921 aggagtcctg caaggataat ctggttgttg aaagtaatgg agacatttac gaatgcgacc
     1981 attttgtcta tccacagtac aaaattggaa acattaataa atctgaactc aaaacgatga
     2041 acagtgtaca actgacagcg caaaaaaaac ggatttcagc gaaatgtcag caatgtgcat
     2101 ataaacctat ctgcaatggc ggttgtccta agcatcgtat tactaaagta aacaatgaga
     2161 ctgtttctta tttttgcgaa ggttataaaa tccttttttc aaccatggta ccttatatga
     2221 acgccatggt tgagttagct aagaacagag taccgcttta ccacattatg gatgttgcaa
     2281 aacaaatgga gaataattaa taacgctctc ttttaactcc attcttagcc tccttcttat
     2341 gaccttaaca aggcacccga cataaattcc tgtcgggtgt ttcgtcatca tcatcttccc
     2401 ttgcgaaaat cctggcttac gtggtatgcg tcagggcatt agcaataccc ttgattatca
     2461 atagcataac cataaccact gaaaattaaa aatttctata tatttcatat tgttaaatga
     2521 tgcttaaata aatttcatca gtaaatcatt gccatagata atcattggat ggcacaatca
     2581 attttcgcta atgataacca ttcccattac gttttgtatg ttgatagcaa agtatctctg
     2641 tctgctgaag cccttttggc tgcgtaagaa taataaaact tcagtgttgt taatcattat
     2701 tatcctcgca atgatccttg gcgtggttaa aattcaggtc tggttaaacg actggaataa
     2761 tgattttttc aatgcgctaa gccagaaaga aaccgataag ctttggcagc tcgttctttg
     2821 gttccctgcc ctgctgggga tctttgtatt aatatccgca aacaaaacat ggttaattaa
     2881 actactgact attcgctggc gcgaatggtt aacagattac taccttaatc gctggtttgc
     2941 agataaaaac tactacttca cgcagatcta cggcgagcat aaaaatacag ataaccctga
     3001 ccaacgtatc gctgaagaca ttctcttact gatcaacaaa acgctcagtc tttcatttgg
     3061 cttcatccag tcacttagca tgctgatcac ctttaccgtt attctttggc aaagcgcggg
     3121 tacgctctca ttcactgttg gtggaacaga atggaatatc caggggtata tggtctatac
     3181 cgtcgtgctc attgtgatcg gtggaacttt atttacccat aaggtgggta aacgtattcg
     3241 tccgcttaat gtggaaaaac aacgtagcga agcgacattt cgaactaatc ttgtgcagca
     3301 taataagcag gcagaactga ttgcgctaag caatgctgag tctctacaac gccaggagtt
     3361 gcgcgacaat tttcatacca ttaaagagaa ctggcatcgt ttaatgaatc gtcagcggtg
     3421 gcttgattac tggcaaaata tttattcgcg ctcgcttagc gttcttccct actttctgtt
     3481 attgccgcag tttattagcg ggcagatcaa tctgggcgga ctgatgaaat cgcgccaggc
     3541 atttatgcta gtatcgaaca atttaagctg gtttatttat aaatatgacg aacttgctga
     3601 actggctgcg gttatcgatc gcttgtatga gttccatcaa ctcactgaat agcgccctac
     3661 gaataagcct aaaaattgcc aacatgcggt acaagtggct aatgcgagta ttcgtacgcc
     3721 tgataataag atcatattag agaacttgaa ctttcatgtt tcgccaggca aatggctatt
     3781 actgaaaggc tactctggtg cgggaaaaac cacactgctt aaaacattat cccactgctg
     3841 gccgtggttt aaaggtgata tttcttctcc tgctgacagt tggtatgtgt cacaaacacc
     3901 gttaatcaaa agcggcttac tgaaagagat tatttgtaaa gcacttcccc tgcccgtaga
     3961 cgataaatcg ttgagcgaag tactgcatca ggttggtctg ggaaaattgg ctgcgcgtat
     4021 tcatgaccac gatcgctggg gagatattct ttccagcggc gaaaaacaac gtatcgccct
     4081 ggcacgatta attttacgac gtccgaaatg gatatttctt gacgaaacta cctctcatct
     4141 tgaggaacaa gaggctatac gcttactgcg tttagtgcgt gaaaaactac ccacaagcgg
     4201 cgtcattatg gttacacatc aacccggtgt ctggaacctg gctgatgata tttgtgacat
     4261 tagcacggtt ttataacaac aataaaagcc ctgctgtacg gagatataaa taaatgaagc
     4321 gagttcttat tcctggcgtc attttatgtg gcgctgatgt ggcgcaggcc gtcgatgaca
     4381 aaaacatgta catgtatttt tttgaagaga tgacggtcta tgctcctgtc cctgtacccg
     4441 taaacggcaa cacgcattac accagtgaaa gcatcgagcg tttaccgacc gggaatggca
     4501 atatcagcga tctgctgaga accaaccctg cggtacgcat ggattcaacg caaagtacct
     4561 cgttgaacca gggagatatt cgccctgaga aaatctctat tcacggtgcg tcgccctacc
     4621 agaatgccta tttgattgac ggtattagtg caactaataa cctgaaccca gcgaatgagt
     4681 ccgatgccag tagtgcaacc aatattagcg ggatgtcaca ggggtattat cttgatgtca
     4741 gcttactgga caatgtgacg ctttatgaca gttttgtgcc ggttgaattt ggtcgcttca
     4801 atggcggggt aattgatgca aagatcaaac gcttcaacgc tgatgatagc aaggtgaaat
     4861 tgggttatcg cactacgcgt ttggactggt taacatcgca tatcgatgag aataacaaga
     4921 gcgcatttaa tcaaggttct tcaggaagta cctatttctc tccagatttt aaaaagaact
     4981 tttatacctt gtcgtttaat caggagctgg ctgataactt tggcgttacc gccggtttat
     5041 cgcgccgcca gtctgatatc acccgcgcgg attatgtttc gaatgacggc attgtcgccg
     5101 gtcgggcaca gtataaaaac gttatcgata ctgcattgag caaatttacc tggtttgcca
     5161 gcgaccgctt tacccacgat ttaaccttaa aatataccgg ctccagccgt gattataata
     5221 ccagcacctt cccgcagtct gatcgcgaaa tgggtaataa atcctatggt ctggcatggg
     5281 atatggatac gcaactcgca tgggccaaac tacgtaccac cgttggttgg gatcatatta
     5341 gtgattatac ccgtcacgat catgacatct ggtacaccga actttcatgt acatatggtg
     5401 atattacagg gcgttgtacc cgtggcggat taggacacat ttcccaggct gtagataatt
     5461 acaccttcaa aacacgcctg gactggcaaa aattcgccgt gggtgatgtt tcgcatcaac
     5521 cctacttcgg cgcggaatac atctattccg atgcatggac tgaacgccat aaccagtctg
     5581 aatcctatgt gattaatgct gcaggaaaga aaactaacca taccatttac cataaaggta
     5641 aaggcagcct gggaattgac aactacacgc tgtatatggc ggatcacatt agctggcgga
     5701 atgtgtcgtt aatgcccggt gtgcgttatg actatgacaa ctatctgtca aaccacaata
     5761 tctccccgcg ctttatgacg gaatgggata tttttgctga tcaaacctca atgattaccg
     5821 ccggttataa ccgttactat ggcgggaata ttcttgatat gggattacgt gatatccgca
     5881 atagctggac ggaatcggta tcaggtaata aaaccctgac gcgttatcag aatttgaaaa
     5941 cgccttataa cgatgaactg gcaatgggat tgcagcaaaa aatcgataag aacgttattg
     6001 cacgcgcaag tgaagcgcat gatcaaatca gcaaaagcag tcgtaccgac agcgcgacta
     6061 aaaccaccat tactgaatat aacaacgatg gcaaaaccaa aacgcattcg tttaacctca
     6121 gttttgaact ggccgaaccc ctgcatatcc gccaggtaga tattaacccg caaattgtct
     6181 ttagctatat caagagcaag ggcaacttgt cgttaaacaa tggttatgag gagagcaata
     6241 ccggtgataa ccaggtggtt tataacggta atctggtctc ttacgatagc gttccagtgg
     6301 cagattttaa taacccatta aagatctcct taaacatgga tttcacgcat caaccgagcg
     6361 ggttagtgtg ggcgaatacg ctggcctggc aagaagcgcg taaagctcgc attatccttg
     6421 gtaagacaaa tgcgcaatac atcagcgaat attcagatta caagcagtat gttgacgaaa
     6481 aactggatag cagcctgacc tgggacaccc gcttgtcctg gacgccacaa tttctgaaac
     6541 aacaaaacct gacgatcagt gccgatattc tca
//
LOCUS       DAJTQQ010000160         6550 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000160 DAJTQQ010000000
VERSION     DAJTQQ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.161"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..334
                     /locus_tag="OO309_003847"
     CDS             <1..334
                     /locus_tag="OO309_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5848807.1"
                     /translation="MTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTG
                     GKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVVACAL
                     ANKLARIA"
     gene            complement(673..1443)
                     /locus_tag="OO309_003848"
     CDS             complement(673..1443)
                     /locus_tag="OO309_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848808.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1488..1922)
                     /locus_tag="OO309_003849"
                     /pseudo
     CDS             complement(1488..1922)
                     /locus_tag="OO309_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(1934..2155)
                     /locus_tag="OO309_003850"
     CDS             complement(1934..2155)
                     /locus_tag="OO309_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848809.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2156..2839)
                     /locus_tag="OO309_003851"
     CDS             complement(2156..2839)
                     /locus_tag="OO309_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR5848810.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(2903..3214)
                     /locus_tag="OO309_003852"
     CDS             complement(2903..3214)
                     /locus_tag="OO309_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848811.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3211..4113)
                     /locus_tag="OO309_003853"
     CDS             complement(3211..4113)
                     /locus_tag="OO309_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCR5848812.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4553..5512
                     /locus_tag="OO309_003854"
     CDS             4553..5512
                     /locus_tag="OO309_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCR5848813.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5512..5907
                     /locus_tag="OO309_003855"
     CDS             5512..5907
                     /locus_tag="OO309_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR5848814.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6090..6470
                     /locus_tag="OO309_003856"
     CDS             6090..6470
                     /locus_tag="OO309_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR5848815.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT         1782 a         1697 c         1590 g         1481 t
ORIGIN      
        1 tatgacaatt cctggtgttg ggccgattac tgccagctta ctatcgtcgc aacttggtga
       61 cgggaaacag ttttcatgca gcagagattt tgcggcttcc acagggctcg ttccccggca
      121 gtatagcact ggaggaaaat cgactctgtt gggtatcagt aagcgaggtg ataaaaattt
      181 acgccggtta ctggttcagt gtgcccgctc attcatgatg cagcttgaac gccagcatgg
      241 aaaacttgct gagtgggttc gggagcaact caacaagaag cattcgaatg tggttgcctg
      301 cgctctggca aataaactgg cccgaattgc ctaggcaatc actactcagc aaaaagaata
      361 tcaggcctga tatatagatg ctgacaaaac tcaattagct tattgcaaac aataagatac
      421 aagttaccgt ctggttttgc gaagattgat tattgatgac atgaacggca aatcgacctg
      481 attaaaaacc taacagggga aaatggctca ctgaagccga ggggattttt aggattatca
      541 ggtgcggaac tcatcgaggc gcgggcatcc cttgcccaca gagacgccgg atagatttaa
      601 gcaagcccac cattaatcaa aaatcagtgt tgcaaaaacg ggggtgacca tagattttcc
      661 ccgccatcgt catcatttcc ggatttttgc cagggcatcc gccatgctgc ggatttcggc
      721 ccgggcttcc cggacaactt cccgcagaac accacggtca aaccaccggc ggggcggttc
      781 atccggtata cgcagcacat cgcaggccag cccactggac aggatatgct caatacaatc
      841 gctgaccaga tacatttcca ccgccgaaaa acgtgcgccc cggttcagga tggcatcgag
      901 caccacgggc aggttttcat cgtcttcatc gagccagcag gggtgatcgc tttccttcac
      961 gaccaggcga aagcggcgcg tctggcgggt cagcaacgtc tgaccgcttg tggtcagcgc
     1021 cgcccggata cgccgcgcat cttctgcccc ccggcaactg acactgcata ctgcatcgcg
     1081 caccgcatca agaactgccc tgatttcgtc ttcggtcagc cattcaccgg catacgggaa
     1141 agacacctgc gttccgtccg ccggaagtac cggcagcgac tgccactgac tgccggacag
     1201 aacaagttcc gctccggttc cggtcagttt ctgaatggcg gaggccaggt gcggcggcag
     1261 cacgccatcg tggcggttaa cgagtaacgt cgccagccgg ggcgcatcca gccgcacgta
     1321 aatgctgatc cagcccggat agttcgggtt acattggtca acatccgtct cttccggcca
     1381 gccctgcgcc agcagcaggt tacgggcgtg acgacaaatc agagcattca gcgtttcatt
     1441 cataattcat gaactccctg tatcgccgcc cacaggcggc gataacatca gtcgttttcg
     1501 tgtgtgttgt ttttcagcag gctgcgggcc tgatcaacca tcgcctgtcg gcgggtaacg
     1561 tattccggga gggggaaacc gtgatcccgg gcagcccatt cacgtttcca cagtgcctgt
     1621 tcagcgatct gagtcagggt ctccagcagt tcagcaggga cggtcacctg gcggttttcg
     1681 cgcacgttgc tgataatctc catcgccgcg gcggaagaaa taccggactc aatccgctag
     1741 tcttccggga tgtcctccag gtgcgccagg acggtcctga tttcatgatc ggacagggaa
     1801 atatccattc cgtcagcgag ggcctgaatc tcttccgggg accagataac cagcatcagc
     1861 ggcgtgtctc ccgaatactt tgctgccagc gcccggcata gcgtttcgca tgttccgtac
     1921 atactctctc ccctcaatct ttgaagcagg aatccagttc cagaatttcg gtatatgcct
     1981 ccatcaagag gcggtttgcg cgaaacaacg tctgacgcac cagacgttcc ggcagggtgt
     2041 cagcattaaa gctgtaccgc cgctccatct cattcatggt tgtacgcagg tcgcacatgg
     2101 cattggccac ctccgccact tcggcgcgaa gtttttcgtg tccggcatag ttcatttacg
     2161 cagcctccgg cataaaccag tcatcattcg cggccccctg ctgcatggcc cgctgtacgg
     2221 cagccagtcg ttgctgtccg gcacggtgat actgctcaag cagctcgata ccgatatacc
     2281 ggcgtccgga ctggagggcg gcgacgcagg ttgaaccgct gcctgcaaac gggtccagca
     2341 ctatcgcatt cgggtgtgtg aagctctcaa tcagcggttg caggctggta acgggctttt
     2401 ccgtcgggtg atggcggttg cccgaatatt tccagcccaa cacgtccggc agcgggtttt
     2461 gtggcagacg tggacggcct tttgccagga tgtaggcgca ttcgtggcga tagcccacat
     2521 atgcggcctt cgatgtgtag tttttggtga agaccaggtg accaacaacg ctgaatcccg
     2581 catttttcca ggcagacata aagcgatcga cgcggttcca gccatagaag ctcaccatca
     2641 gcgcgtcttt tttgagtacg cggtacattt cattacaggc cggttgcagc cattcatcag
     2701 ttttatcacc agcaatggta cgcccctgac ggtcacgaaa accgacgaga tatggcggat
     2761 cggtgaggat gaaatcaatg gcgttatcag gaatacgggc cataacatcg atgcagttac
     2821 cgaggacaaa acgggacata acagatcctt ctcttacaga gggcacccct gtggtgcccg
     2881 ttccccgctg cctgtgggct gttcatcgct tccggtaacg ggtcaggccg accgtggaat
     2941 accgcagccg tcgtcttttt gacagcaagg ttatgccgcg aaagcggctt gaagggcgtt
     3001 catcagaaaa gggcgacggg ctggcgtaca ctgtcaccga caccagccgg gagcggtaaa
     3061 agcgcccgtc ccgtgcccgc cggacgcggg acactgacca caggcagcgt ttatgccgcg
     3121 ccgcgtcagc cggcgcagta aaaagaggag cacggcgaca tcctttgtcg gaaaagaaac
     3181 gggcggttgc ccgcccgtct ccgtcatact tcatccgcca taatgcgcca cattgtcgac
     3241 tatccacgca ggtccggtga cttccggctg ctcatcgtca tctgtcgggg aagaccattc
     3301 aagttccccc cacagcacat tgacgagctc tccacgctca tacaactgcc agccacagaa
     3361 atcacagccc tgttcggcat accagtgctc cagcgtgcag ctgaaacggc ggcttaattc
     3421 tgcaataacg tcgctttccg gctgacacca cggagtgtcg aaatccacct gaatgaagtt
     3481 ttctccctga ctgctgatat tcaggtcata tccacaaggc cacttcacgc cataccgttc
     3541 cgtataccag tgatatgcag aaggaacacc gttcagaaca ccaccattaa agccgttcac
     3601 ttcaacatcc aggcgggtcg gcagaaccgt cagcaggtca aacggcatgg ttttttccgg
     3661 cactacgttg tcacacagac ggttccacca caccgataca tcctcgttac cccatatgtc
     3721 acaccagtca cctctttttg cggtgaaatg cacagtgatg gtttccctgg catcatccgg
     3781 taatccttcc caacgacgct ggccagtacc gctctgtaac cagagttcat gcagcatcag
     3841 gcagttttgt tcatccagca acacaccgtt ctgcaggtgt gtcagccagc gggtgaacgc
     3901 gatattttcc gtcgatacag caccacgtcc ggcagccgtc actccggggc actgttcaga
     3961 gcggatattt tcagtgatct gcagaagtcc ggcacttccg gccagaaaca gctgaatacc
     4021 ttcatttgtg gcgcggcgat aaagcggtgt gactgcaccg ctggccagtc gtttaatctc
     4081 agcgatctgt gccggctcac caggaaaata catacgattc gagcaccagt ttggcatggg
     4141 aattctcctc tcacatgcgg cctccatcag gccgtgccgc acctgtaacc gggcgaccgc
     4201 atacgaatgc aggtgcggcc ccgaagccgc agcgcgtgga ggaggaatga cgaaacgtga
     4261 ccgggtgagg aggccggcga cagcgacaca cttgcatcgg atgcagcccg gttaacgtgc
     4321 cggcacggcc tggttaaata gtatccgtct cagttcgtgg cgcaaaatga gaaaccaata
     4381 agaactcaaa atgaaccaca acaagaacat aaagagactc taaaagagca acaccgtttg
     4441 acatagactc aaaatgagtc acaataagaa acaataaaaa actcaaaaag atgcaccgac
     4501 ataaaggatg ctcaatttga gataaacaca aactcaaaac gaggcaaaaa gaatgctaaa
     4561 agtatcatgc gatgatggtt caactaacgt aaaactggcc tggcttgaag atggcgaggt
     4621 aagaaccagt ctgtcaggaa acagctttaa agaaggctgg aacccgggac tgtttaatgc
     4681 cggcaaagtt tacaattacg tcgtggatga aaaaaaatac acctatgatt tgggaagcac
     4741 tgctgtcata ggtacaacac atgtcagtta tcaatacagc accaccaatc tgcttgccat
     4801 acatcatgca ctgctgacat ccggccttca gccacaggat gtagaactga cagtaacatt
     4861 acctgtgacc gaattctttg ataatgacaa tcaaccaaat gaagaaagaa tagaaagaaa
     4921 aaaggcaaat gttctccgtg agatatctct gaataaaggg gaaacattta aaattaaaaa
     4981 agttaatgta atgcctgaat ctttgccagc tgcatttgaa tcactaaaaa aagataaagt
     5041 aaacaaactt gaacgcagcc tgattatcga tcttggagga accactctgg attgtggttt
     5101 aattctcggt gcatttgaag gcatttctga aatccgtggc tattcagaaa tcgggacatc
     5161 ccgcatcact cataccgtca tgaatgcgct gacgaaagcc tccacaccat gtaattattt
     5221 tattgctgat gagttaataa aaaacagaca cgataatgaa tatctccaga cattaataaa
     5281 tgatgtagct gaaataaaaa acatttctca tgtaatagac agagaagtga aatctctggc
     5341 agaaagcatc aggcaggaaa tttcaacatt ctcaggaatg aaccgaatct atctcactgg
     5401 cggaggagcc gagcttattt accctcacat aaagcaatat ttcccgaatc tgaaagtgaa
     5461 caaggtggat gaacctcagt ttgcattggt gaaagcaatg gtgcatgcgt aatggaaagc
     5521 agtgatccaa aaaaaagaaa gaaagttgtg gcatacctgc acccggcact ttatccgcag
     5581 gacaacctga cacaacaaac aatagactct ctcccagtac aaatgcgggg agatttctac
     5641 agacaatccc ttatttgcgg agcagctctt tattctgttg ccccccgact gttaacgcta
     5701 atcagcgttt ttttcagtga gaaaatcact gcagaaaatc tggtgaaact tatagaacaa
     5761 acaacaggat acacatccac atcaattgat ataagtgtgt taaaaaacat catagaggca
     5821 tcttcagaaa acaaatcaga gagcatcact tcaaaagatg atttcgaaga acaaaccaga
     5881 cgcaatcttt ctatgttaaa aaaataataa aagaagagca ggttcagtca attacctgct
     5941 cttcttttat acatactaaa aaatcagatt tttacgaata ttcagattat cataaaaaat
     6001 gcaacagaca ggaatgcaat aattttgcat tatctcgttg cttgtaatgc aaatatgcat
     6061 tattgtcagt gtgtgacaat aacagagaga tgacgatggc gaccattaac gcacgtattg
     6121 atgatgatat aaagaaccaa gcagatgaag tgctgaagct gatgaatatc agtcaaactc
     6181 aggctattgc agctttttat cagtatatta ctgaacaaaa gaaactgcca tttgtgatca
     6241 cctcaattgt aaaaacgccg catgatttat tacgtgaaag tacagacatg ttagctgaag
     6301 ctcttgcagt gatttccaat ctacaggtgt ggactgaaca gcaggatggg attggaaaag
     6361 caaagctaat ggagtattac cgccgcctcg acgcactgta ctgttgtgca aaagagaaaa
     6421 taggtcttct ttcagataac cgggatgctg aactaggctg tgtcccttaa tcatctgagt
     6481 tatagtaact gtctgtttac acaacatatt gaactatggc tcgctacgac cttcccgatg
     6541 aggcatggac
//
LOCUS       DAJTQQ010000161         6509 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000161 DAJTQQ010000000
VERSION     DAJTQQ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.162"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..1039
                     /locus_tag="OO309_003857"
     CDS             <1..1039
                     /locus_tag="OO309_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5848816.1"
                     /translation="AICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCDTIVEAAA
                     LLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYFAMNNWSF
                     LQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDIN
                     KNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRV
                     SLSIDILYFITSSRKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMTGLYNRKI
                     LTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLHDAYKASDERL
                     YVNKQNKNSRS"
     gene            1082..1585
                     /gene="yeaK"
                     /locus_tag="OO309_003858"
     CDS             1082..1585
                     /gene="yeaK"
                     /locus_tag="OO309_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR5848817.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1586..1690)
                     /locus_tag="OO309_003859"
     CDS             complement(1586..1690)
                     /locus_tag="OO309_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848818.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1860..2306
                     /locus_tag="OO309_003860"
     CDS             1860..2306
                     /locus_tag="OO309_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR5848819.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2263..3081)
                     /gene="nimR"
                     /locus_tag="OO309_003861"
                     /pseudo
     CDS             complement(2263..3081)
                     /gene="nimR"
                     /locus_tag="OO309_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            3178..4358
                     /gene="nimT"
                     /locus_tag="OO309_003862"
                     /pseudo
     CDS             3178..4358
                     /gene="nimT"
                     /locus_tag="OO309_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4413..4760
                     /locus_tag="OO309_003863"
     CDS             4413..4760
                     /locus_tag="OO309_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR5848820.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4782..5036)
                     /gene="yoaF"
                     /locus_tag="OO309_003864"
     CDS             complement(4782..5036)
                     /gene="yoaF"
                     /locus_tag="OO309_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCR5848821.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5219..6244
                     /gene="dgcP"
                     /locus_tag="OO309_003865"
     CDS             5219..6244
                     /gene="dgcP"
                     /locus_tag="OO309_003865"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCR5848822.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVLWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6277..6375
                     /locus_tag="OO309_003866"
     CDS             6277..6375
                     /locus_tag="OO309_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCR5848823.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGCYAAPGA"
     gene            6378..6452
                     /gene="yoaJ"
                     /locus_tag="OO309_003867"
     CDS             6378..6452
                     /gene="yoaJ"
                     /locus_tag="OO309_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCR5848824.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
BASE COUNT         1572 a         1579 c         1595 g         1763 t
ORIGIN      
        1 cgccatctgc gaggcttttg agtctgccaa taatacccat ggcttaaacc tgactgccca
       61 taaatacccg ggcttacgcg gcacactaca aaccgcatcc actgactgcg acacaattgt
      121 ggaagctgca gcactattac ccgcttttga tcaggcagtg gaaggcaacc gccaccagga
      181 tgattacggt tcaggtcttg ggatggccga agagaaattt cactattatc tcgatctcaa
      241 tgaccgctat gtctattttt atgagccggt taatgttgaa tactttgcga tgaataactg
      301 gtccttcctg cagtcaggaa gtattggcat cgatcgcaaa gatattgaaa aggtatttac
      361 cgggcgtacc gtattgtcga gcatttacca ggatcagcgt actaaacaga acgtgatgag
      421 tttgctgacg ccggtatatg tcgcagggca gctaaaaggg attgtgctgc tggatattaa
      481 caaaaacaat ttgcggaata tcttttacac tcatgaccgc cctctcctct ggcgttttct
      541 caatgtcacg ctaaccgata ccgattcggg gcgcgacatt atcatcaacc agagcgaaga
      601 taatctgttc cagtatgtca gttacgtcca tgacttacct ggcggcattc gtgtctcgtt
      661 atccattgat attctttact ttatcacgtc ttcgaggaaa agcgttctgt tctggatttt
      721 gacggcgtta attttgctga atatggtccg gatgcacttc cgtttatacc aaaatgtgtc
      781 gcgagaaaat attagtgatg cgatgactgg actgtataat cgcaaaattt taacccctga
      841 actggagcag cggttgcaga aactggtgca atccggttct tcggtgatgt ttattgcaat
      901 tgacatggac aagttaaagc aaataaatga caccctcggt catcaggagg gggatttagc
      961 gattacgtta catgatgcgt ataaagcttc cgatgagcgt ttatatgtca ataagcagaa
     1021 caaaaacagc cgttcatgat aaccttctgt ggttgtttgc ttgtaatctc aggagcgtga
     1081 aatgactgaa atggctaaag gaagcgtgac gcatcagcag ttaatcgctt tattatcaca
     1141 agaaggtgct aacttccgcg tcgttaccca tgaagcggta ggtaaatgtg aagcggtgtc
     1201 agaaattcgt ggtaccgctc taggccaggg tgctaaggca ctggtatgta aagtcaaagg
     1261 caatggcgta aatcaacatg ttctggcgat cctcgcagcc gatcaacagg ccgatctgag
     1321 ccaactcgcc agccatatag gcggattacg cgcttcgttg gccagcccgg cggaagttga
     1381 tgaactcaca ggctgtgtct tcggcgcgat cccccctttc agcttccatc caaaactcaa
     1441 actggttgcc gaccctctac tcttcgaacg atttgatgaa atcgccttca atgcagggat
     1501 gctggataaa tccgttattt tgaaaaccgc ggattatttg cgcattgcgc aaccagaact
     1561 ggtcaatttc cgccgcactg cgtagctagc ctatccgttc aataagcaga acggacaaaa
     1621 ccagcacaac cgcaatagca aaaaacgatg atgtgataat cagtgtctcg acgaacattt
     1681 gatcgttcat agcatgcccc cgggagatag ctgttttcac gttattgtta gcgtgcacaa
     1741 atggcagttt gatgacagtt cgcctgattt tttatgcaaa aaacgtaaag attttctact
     1801 tccttcctgc agcaagcata aagtaagcag gcttattatt ttttggcaag gaaaccacga
     1861 tgtttgatgt cactttgctg atcctgctcg ggttagctgc gctgggcttt atcagtcata
     1921 acaccactgt cgccgtttca attctggtgt tgatcattgt ccgcgtcaca ccgttaagca
     1981 cctttttccc gtggattgaa aaacaggggc taagtatcgg gataatcatt ctgacgattg
     2041 gcgttatggc ccccatcgcc agcgggacgc taccgccctc aaccttgatt cactcgtttc
     2101 tgaactggaa atcgctggtg gcaattgcag taggcgttat tgtctcctgg ctgggtgggc
     2161 gcggcgtgac gttaatgggc agccagccgc aactggttgc cgggctgctg gtgggcactg
     2221 ttttaggcgt agcgctgttt cgcggcgtac cggttggccc gcttattgcc gcgggtctgg
     2281 tttcgctgat tgtggggaaa cagtagttaa tcccgcaata tatcggcctg gcgtttgccc
     2341 caagcctttc ttaaacatag taataaaggc cgttgttgaa tcatacccca gcgtatgagc
     2401 aactttctgt acagtatcgc ctttcactaa tccctgaagc gccataatca gctgtaactg
     2461 ctgacgccac tggcgaaaac tcaaccccgt ctctttcact atcaggcggg caagattgcg
     2521 ttcactcatc gcaaaaaagc cagcccattg ccccaatgcc ccccactcga cgggcccttt
     2581 cgccatcatc tccaccatcg tgcggatttt aggatgagaa gaaacgggca ggtgcagctg
     2641 ttgttgtggc tgctgaggga gttcatcaaa caggacctga attaatctgc gggtcatcgg
     2701 ctcagcacgc tgagtcgtcg tcctgtttgc cagggttaaa atcaattccc ggcataaggg
     2761 cgatattttc aatgtgcaac atggtcggca ttgtcactgc gctgggttcg ataaacagaa
     2821 aacagagttc agcgttagct gtcacttgat tgctatgctt caccccgccc ggtatccaaa
     2881 ccgcatattg tggcggcacc atccacaaag cattttccac cgtacaggta attgcaccat
     2941 gtaatgccag tatcagctgt cctttgcggt gctgatgtac aggactggtt aactcatccg
     3001 ttccggcgtg aatacaaaat gcgaccgcgg cttcatggtg tcggtgggtt catagccatt
     3061 gagattcaac ctgtgcatca ttttgtccga acttagcgat aatttgtcat tttagcttga
     3121 ttcaacataa caataaaaac ggtaaggtac agcctcgttt gtaacaatga gaagcatatg
     3181 acctgttcaa cttcattaag cggcaaaaac aggattgtcc ttatcgctgg cattctgatg
     3241 attgccacaa cattacgcgt cacctttacc ggcgcagcgc cgttactgga tacgattcgt
     3301 tccgcttact cgctgacgac agcgcaaacc ggcttattaa ccaccctgcc attattggcc
     3361 tttgcgctaa tctcacctct ggctgccccg gtggcgcgac gttttggtat ggaacgtagc
     3421 ctgtttgccg cgttactttt gatctgtgca ggtatcgcaa ttcgttctct cccttcgcct
     3481 tacttattat ttggcggtac agcggtcatt ggcggtggga ttgcattagg caatgtctta
     3541 ctgccaggat taattaaacg cgatttccct cattccgtcg ccagacttac cggcgcatat
     3601 tccctgacaa tgggagctgc agcggcactg ggatcggcta tggtcgtgcc gctggctttg
     3661 aacggttttg gctggcaagg cgcgttgttc atgctgatgt gttttcctct gctggctctt
     3721 tttttatggc tgccacagtg gcgaagtcag caacatgcaa atttgagtac ctcacgcgcc
     3781 ttacatactc ggggtatctg gcgttcgcca cttgcctggc aggtaacatt gtttcttggg
     3841 atcaactcac tggtctatta cgtgattatt ggctggcttc cggcgatcct catcagtcac
     3901 ggctatagcg aagcacaggc gggttcactg catggtttgc tgcaactagc cacagcagct
     3961 cccggtttgc tgatcgcact tttcttacat catgtgaaag accagcgtgg tattgcagcg
     4021 ttcgttgcct tgatgtgcgc agtgggcgcg gttgggctct gctttatgcc agcgcacgcg
     4081 atcacctgga ctctgctttt cggttttggt tccggcgcaa caatgatact ggggttgacg
     4141 ttcattggtc ttcggcaagt tctgcgcatc aggcggcggc actctcgggg atggcacaat
     4201 ccgtcgggta tttgctggca gcctgtgggc cgccgctaat gggtaaaata cacgatgcta
     4261 acggtaactg gtccgtacca ctgatgggtg ttgcaatact ttcactactg atggcgattt
     4321 tcggactttg cgccgggaga gacaaagaaa ttcgctaata tccggtgcta tagtgacgtg
     4381 acaaatcttg cgtgaaaggg agaacaaaca cgatgaatat tcagtgcaaa cgcgtttatg
     4441 atccggctga acagagcgat ggttatcgcg tactggtcga ccgcctctgg ccgcgcggta
     4501 tcaaaaaaac cgatttagcc cttgatgagt gggataaaga aatcacgccg tcaacggaac
     4561 tgcgcaaagc ctttcacggc gaagtcgtcg attttgcaac ctttcgcgag caatatcgcg
     4621 cagaactggc gcaacacgag caagaaggaa agcggctggc ggacatcgcc aaaaaacagc
     4681 cgctgaccct gctctacgca gcaaaaaaca ccacgcagaa ccatgcgctg gtgctggccg
     4741 actggctacg tagcttgtga ttttagtacg gcatccggcg gttatttttc accagccgga
     4801 tggtcccgtc gccacaatgc ccattcatca atcgtttcac cgcccggtaa tttgcaattg
     4861 ttgctgaccc cttgcgctgt ctgcactgga atgagcgtcc cgcccttctg ctggcaatag
     4921 accgacgccg gatttgccat accaatctgc ggcggtttag gtgcttctgg ctgagaaggg
     4981 gttgaacaac cagccaggac cagcaagcaa ggcagaacaa aactgataat tttcatttat
     5041 tgatctcaca tatttatcca agcttagagt atcgcggtat cgttttgttt tgcagcacta
     5101 tttttattac attcactcaa aacatattac gtcttgtttc atctttgttg atgatgtttt
     5161 atcatgcctg caaagattaa ataatcagca cttacccgcc gtatcctgga gttgttccgt
     5221 gtcagatcag attatcgccc gcgtctcgca atcccttgcc aaagaacagt cactggaaag
     5281 tctggtccga cagcttctgg agatgctgga aatggtcact gatatggaat caacctacct
     5341 gaccaaagtg gatgtcgaag cgcgcctgca gcatattatg tttgcccgta acagccagaa
     5401 aatgcacatc ccggagaatt ttaccgtctt gtgggattac tcgttatgca aacgcgccat
     5461 tgatgaaaac tgctttttca gcgatgaagt ccctgaccgt tggggcgact gtattgcggc
     5521 acgcaatctt ggcatcacca catttctgag cacgccaatt cacttaccgg atggatcatt
     5581 ctatggcacg ctttgcgccg ccagcagtga gaagcgccag tggagtgaac gcgcggaaca
     5641 ggttttacag ttattcgccg gactgattgc acaatatatt caaaaagagg cactggttga
     5701 acagctgcac gaagccaatg ctgcgctgat tgcgcaatcg tataccgact cgttaaccgg
     5761 gctaccgaat cggcgggcga tttttgaaaa tctgacgacg ctgttttccc tcgcccggca
     5821 tcttaaccat aagataatga tcgcgtttat cgatctggat aacttcaaat taatcaatga
     5881 tcgttttggt cataatagtg gcgatctgtt tctcattcag gttggcgagc gccttaatac
     5941 gctccagcaa aatggcgaag ttattggtcg tctcggtggt gatgagtttt tggttgtttc
     6001 actaaacaac gagaatgcgg atatttcgtc gctgcgagaa cgtattcaac agcaaatacg
     6061 tggagaatat cacttaggtg atgttgattt gtattatccc ggtgccagtc ttggcatagt
     6121 agaagtcgat cctgaaacaa ccgatgcaga cagtgccctg catgctgccg atattgcgat
     6181 gtatcaggag aaaaaacaca aacagaaaac accttttgtc gcgcatccag cgctacattc
     6241 ctgaggcgta ttcacatcct tttgattggt gataacatgc gaatcggtat tatttttccg
     6301 gttgtaatct tcattacagc ggtcgtattt ttagcatggt tttttattgg ctgctatgct
     6361 gccccgggag cataaagatg aaaaaaacaa cgattattat gatgggtgtg gcgattattg
     6421 tcgtactcgg cactgagctg ggatggtggt aacgtcacct ctaaaaaata gcaaaggctg
     6481 cctgtgtgca gcctttgtgc aatttaagc
//
LOCUS       DAJTQQ010000162         6200 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000162 DAJTQQ010000000
VERSION     DAJTQQ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.163"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO309_003868"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO309_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5848825.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="OO309_003869"
     CDS             2089..2238
                     /locus_tag="OO309_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848826.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(2983..3423)
                     /locus_tag="OO309_003870"
     CDS             complement(2983..3423)
                     /locus_tag="OO309_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5848827.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3641..4030
                     /locus_tag="OO309_003871"
     CDS             3641..4030
                     /locus_tag="OO309_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848828.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4074..4478
                     /locus_tag="OO309_003872"
                     /pseudo
     CDS             4074..4478
                     /locus_tag="OO309_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4588..5421)
                     /locus_tag="OO309_003873"
                     /pseudo
     CDS             complement(4588..5421)
                     /locus_tag="OO309_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(5421..>6200)
                     /locus_tag="OO309_003874"
                     /note="aph(3'')-Ib"
     CDS             complement(5421..>6200)
                     /locus_tag="OO309_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR5848829.1"
                     /translation="GESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGER
                     DRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLG
                     AVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELP
                     VRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEE
                     NWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT         1433 a         1617 c         1641 g         1509 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagccacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgttgcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcat tccttcagct catacgtcac tttcatcctc
     2641 tgtgccattt gtacatcacc cccttcctga acgtgctgac agcgggtttt ccggctgcgc
     2701 cggcaacgct tcacggccac gcaataaatt gcgtccccgt tcaccgtttt cggatgggaa
     2761 gaacgccgga aacaggactt accccggctg aatggtgtga atttccgcgc tatgcacttg
     2821 tgcgcatact catgcatgcc gtaaaaacag agcctgcgcg tttctggcgg gttttcgggt
     2881 ggtttgttgc ctgttttacc ggtttcccgt cagaaacgcc ctgagggcct ctcaggcggt
     2941 gctgataaca gatgttatgg taaatcagag tcacctgcac tgttactctg aaccggagcg
     3001 aaacagccgc gaccagaatc cccgcttttt ttcctgtgcc agcgcgtcag ccagacggct
     3061 aatttcggcc tgtagttgtt ccctctcctc atttcgcagg cggttttctg cttcccgcgc
     3121 catcttgtct tcaagcatta gggtaacagt ctgctgcaga gcgtccagtt tctgctgaat
     3181 gacgtctagc tgagcatttt ccgcctggct aacgacagat ttggagtgtc cctccttgtg
     3241 cctttttggt gtctcagaag tgcgcagctc gccataggcc cgaatcagtt cactggtctc
     3301 gaactcccgg cgatcatttt ttcctgtgcg atagctgaca aggcctttag ccatgtcacg
     3361 atataattgc gagcgcgact ttcctgttaa tttcagtgtc tcttgaactg tgtgccatgc
     3421 cataaaagtg acagtgtctc ctgtgtgtac cgtattgtaa cagcagaaca tattactgtt
     3481 ttaacacaaa aggtctgagg ctgcccgtga ggcgtgaaaa atacacaaaa aacctgttga
     3541 cggtgtggtt ttgacgggcg tagtatttcg aatactgtat ataaacacat tgttataaat
     3601 acagtttttg ctgtttttaa aagtcaaaga ggataatttt atgagtacag agttaaatag
     3661 cggtactgtc ggtggttttg gtcacatggg accaaatgac ccctacaatg atggtcatag
     3721 cggctctgga agttcgggag gtagttttaa cggtggtggt aatggtggcc atgataatag
     3781 tagtggttca tcttatactg catcctttgg tctcatcact ggccgacacc cgataactgt
     3841 cccaggtacg aagaatatta attctgtcga tctttctgct gtcattaata acattataca
     3901 tcatgaaggt gcacgcaata acatgtacct agatactgcg aacaatgtaa gagtgggagt
     3961 gacgggcact ggctggcaat gtctagcaac ggcaggcatt tcggctgagg gtaaaagaac
     4021 tttccgctaa gcgatagact gtatgtaaac acagtattgc aaggacgcgg aacatgcctc
     4081 atgtggcggc caggacggcc agccgggatc gggatactgg tcgttaccag agccaccgac
     4141 ccgagcaaac ccttctctat cagatcgttg acgagtatta cccggcattc gctgcgctta
     4201 tggcagagca gggaaaggaa ttgccgggct atgtgcaacg ggaatttgaa gaatttctcc
     4261 aatgcgggcg gctggagcat ggctttctac gggttcgctg cgagtcttgc cacgccgagc
     4321 acctggtcgc tttcagctgt aagcgtcgcg gtttctgccc gagctgtggg gcgcggcgga
     4381 tggccgaaag tgccgccttg ctggttgatg aagtactgcc tgaacaaccc atgcgtcagt
     4441 gggtgttgag cttcccgttt cagctgcgtt tcctgtttgg ggtcgtttgc gggaaggggc
     4501 ggaatcctac gctaaggctt tggccagcga tattctccgg tgagattgat gtgttcccag
     4561 gggataggag aagtcgcttg atatatagta tgacgtctgt cgcacctgct tgatcgcggc
     4621 cgcgatagct agatcgcgtt gctcctcttc tccatccgcg ttccaagctg cggaaaggca
     4681 cccataagcg tacgcctggt cgagcaggcg acgcggatcg acgtccagcg cacgagagaa
     4741 tgcgtccgcc atctgtgcaa tgcgtctagg atcgagacaa aggtcgtctc tgtcagccgg
     4801 atcgtagaac atattggcgg cgccaaagcc cacttcaccg accagaccga cgggatctat
     4861 caccagccag ccgcgactgg agaacatgat gttttcatga tgcagatcgc catgtagccc
     4921 acgcagttcc gaggcattgc tcatcatttg atcggctata atcgccgcgt ggacgtagtc
     4981 agtttgacaa cctgcgtttt gatcatcgcg cgcccgctga aacaaagctg caaagcgatc
     5041 ccggatcggg agaagggcag aaggcagggg ttcctcagat gcggcataca gcttcgccat
     5101 tagttccgct gcaatttcgg tcgcctggta gtcgccgtgc tcggcaacga tgtgagagag
     5161 cattcgctcc ccggcatatt cgagcaacat cagattgttc tcacgaccga gcaaccggac
     5221 tgctcccctc ccattgcgcc ataccagata gtcggccccg cgcagttcat cagcaatgtc
     5281 ttctataggt ttcaatccct tgacgattgc aggagtcccg tctggcaatg aaactttcca
     5341 aacgaggctg gaaaaggtgt ccgcaatgag aacaggttgc gaaacgtgcc aatgagcagg
     5401 aaaaacaggc ggcatgaaca tcaaccccaa gtcagagggt ccaatcgcag atagaaggca
     5461 aggcgttcgc ggtcgggggc ttcgatcccc aatacattga ataggacagc gaaggcgcgc
     5521 tctgcttcat ctggcgctgc ccagttctct tcggcgttag caatcatgag tgccaaatcg
     5581 gcatagcgat ctgctgttcc gagccgccca aggtcgatca gacccgtgca ttgaagagtt
     5641 ttagggtcca ccatgaagtt cggcatgcag ggatcaccat ggcaaacaac catatcggtg
     5701 cgctcttggt cgagccgcac cggtagctct cgttcgacac gagccaaaag atcgagctgc
     5761 ggcgtactct tgtcctcgtc cggtaagaag tcgggattga cggcattgcg ggacaccaca
     5821 tcaacggcgc gtccgaacat tcgcgacagc ctgcgctcaa acggacattg atcaaccgat
     5881 aggctgtgaa cagcgccaag ttgctgcccc attgacggcc acgctttgag caaatccgct
     5941 ccagacagat cagccgccgg tactcccgga attgccgtta tcaccaagca tgcaccctcc
     6001 tgttcctcct gccagttgat gacctcgggg caagccacac ctcgaccttt gagccaaatg
     6061 aggcggtcac gctctccagc gagctcaccg cggcgggaag caggtgcgat tttcgcgaag
     6121 gcatgcccgt caccacgtcg aaaaacaaaa tcaccagatt ctccgcctct gacaggcaac
     6181 cagtcagaat gcgattcacc
//
LOCUS       DAJTQQ010000163         6103 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000163 DAJTQQ010000000
VERSION     DAJTQQ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6103
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.164"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(1..393)
                     /locus_tag="OO309_003875"
                     /pseudo
     CDS             complement(1..393)
                     /locus_tag="OO309_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO309_003876"
                     /gene_synonym="virG"
     CDS             complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO309_003876"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR5848830.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4416..5618
                     /gene="virA"
                     /locus_tag="OO309_003877"
     CDS             4416..5618
                     /gene="virA"
                     /locus_tag="OO309_003877"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR5848831.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5797..6103
                     /locus_tag="OO309_003878"
                     /pseudo
     CDS             5797..6103
                     /locus_tag="OO309_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1869 a         1388 c         1050 g         1796 t
ORIGIN      
        1 tcaggctgta cgcttcccca tgtgatttgc ctcgccactt caccgggctc aggaccccgt
       61 aggatcttca ccgaaaaaaa tgcatcacca gttgctttat tatttgtttt aggtagacct
      121 gttactaatt tatacctact ctgaccaagt ctggataaga aaggcacacc tgcacatttc
      181 tacttggaaa tgttttcttt taccattttg ctcaaaaatt tcaacatcca atattcctcg
      241 tacagaacta ctcaagtcct gaatagaaaa agttccagct ggtacagtag agtcatatat
      301 tactcgacct tgttgagata caacaacacg tgcattagtt tctgcaatac cagtaatctg
      361 aggcgcataa ccacgaatat ttggcggtag cattcgttca tcgctttcta atgcaattcc
      421 tgtgtaacgc caagcaacag cgcattctgg cgcatgatct ggtaaacacg tttggcattg
      481 atcgcaggca taccatcatg tgcacaaaaa cccaccgagc ttttacacgg tgggtcccag
      541 agaaatgcag gacatcaaca cgccctgcat ttttattatc agaaggtata tttcacaccc
      601 aaaatacctt gggtgtctct gtaactgtta ttacccaatt gctgagatac gtttccccag
      661 atgcttaggt tatctgcaag acgccccccc acaccagttt tgagttcaat cacattacgg
      721 ttgcctatct ggtgatttga catgccattc ataattacac catattgctt tgagctccat
      781 ttccaattca cttccaaaaa tggttcgaac ttacgaagag tctccttatc gatacaactc
      841 tgactaatta aggatgtacg caatcccaat tttgcttgaa tattattgcc cccagaggat
      901 gtgaccatag tgccattgtg ttcaatatag tcgtttgctt ttaccccatg cctagttatc
      961 tggacttgtg gattgagata caatgcgttg tcaatattat tatgagcagt ccagcgtatg
     1021 gttgggtaga tatagccagc ttccaaagct cctgttattc ctttgctgga atatttctca
     1081 ccagttaacc catctccttt gactgtatta ttaaaccaac tgtactgcaa ccaagcatcc
     1141 atatatagac ctgttcgttc tttttcatcc tgaaaccaag aactgtacaa accggcagta
     1201 taaccgctca cagttcctcg tgaactataa ctagtatact tattatgagt tttgctatcc
     1261 tgatatccat aagctcccat aatacccaca tgcaagccat catgatcagt gaagtttgtc
     1321 ttcaaaatat ctccaccaat ctgataaacc atcctgttag ctgtggtttt taattgtcta
     1381 tcagctaaat tatgctcatt atgtccacca acagtacgaa tccacatggt ggtttcgctg
     1441 agctgacctg tagtaggatc aacaatagtg ctaccgtgtc ggtcatagag agaaggacta
     1501 aacatcgtgt tcattgctat caggttagca atataactgc cagcttcagg gcgccatgtg
     1561 tgaatactac cttcacccaa attaagtgaa gatacaagct gtgaactagc tcggcgacta
     1621 ctcatttgag tagactcttg attgctcatc tgttttgatt cttgattatc catctgacta
     1681 gttagatacc acttattggt attcagtcca gatacagtgc cctgtttcag gcgatagtca
     1741 taactaccag caacaatacg gcctttctga ataaaagcat tcttatcaga agaatctgtc
     1801 gaaataattt gaacaccttc aagagtttta tctccagagc ctccctcatt tgtcactgca
     1861 acataggtaa taccagacgc atggcctgca atttccagcc ggtcagtttt actgttgtca
     1921 ttgccgagaa ccgcgcccaa agataatatt ccgccctttc catgccaatt acctttctga
     1981 acataggtct gaccgacatt tgacgaacta ttattgagaa taacatgacc gtttttctcc
     2041 agagtcatgt tacctgtgac aatgacgggg tcaggcaaaa aaggggcatt aatattattg
     2101 attagcacag tcccactatt taataaagtg ttaacagttt ggttcatgcc tgaaaaattc
     2161 aaggtagcac ctttattaac aataacacca gcggtacgtg tcatagcttc aactgtcccc
     2221 atttttagaa tgcctgcatt gatgttggtg aaaccagagt aggtattttt ttccgccaaa
     2281 atgagagttc cctcccctga ttttgttagg gattttccgt cccagctatt tatattatga
     2341 tggctgagat tatctgccag aatagtacta acagtaagtt cagcgttttc tttcagattg
     2401 aactctccat gactgtcgcc atctgtatca ttccagctca aaccgtagat caatttattc
     2461 tggtcactga tatagctttt tgtatagtca atagcaccag tagtgagtaa cggattgatg
     2521 atagatatat tagaaacact gaaacctgtt atccctttat ctgcacttag cagtttatag
     2581 tccttctgcg cccagttatt tataatagcc tccactttta cattgttgcc aaatgacata
     2641 tttccagaag cttccagact actgattcca gctaaattta aagaactgtt ctcaccaaaa
     2701 gaaacgtcac ctgaaacagt gaatttgtta cctgatagtg aaacagttgt acaatcacca
     2761 gcacataaat caccttcaat agttggtgtg gtcccagtgt aatcattgtt gataattttc
     2821 agaatggatg aattgttaac ctgtacatca ccagtaatga cagaaccttc atggagtatt
     2881 aatgaattgt ttccaccagt gatgtggata gcattacctg ctacattttg tgttccatca
     2941 tcttctttac ctgaaattat atatccgcta ttagcaatgg tcatattgct accatttata
     3001 gcatcccccc aatgtccacc attaccgcct aaaatagctc cattgttgat tacagacagg
     3061 ttatctcctg tgatagcatt accaccgtag ccatcataac cataaccgta agcagcacct
     3121 ccgttgcctc cagcgaacgt gcccttattg attacagaga ggctgcttcc tgtgatggca
     3181 tcacctccat taccgccatc accctcacca taattgttac ctccattacc accggaaata
     3241 gtccctttgt tgattatggg taaattgact cctgtgacgg catcacctcc attcccccca
     3301 tcactatcac tataactatc gccaccatgt ccacctgaaa taatttctcc atttattata
     3361 aacagattgt ctcctgtaac agcctcacca ccatcaccgt tatggtcagc accgctaccg
     3421 ccgctaccac caagaatcat gccttgattg attatagaca agtcagatcc ggtaatagag
     3481 tcaccaccat taccgccaca actattacca tcattattat caccagcacc accatgacct
     3541 gcgctaatca taatatcaga gttaatccgt agcttagttc tagatgcatg agaggggttg
     3601 gaagaagtta tataccacgc atccattcca tcttcaccag ctcccaaggg agaaagtcca
     3661 tcaacaggtg ttaataattt ttcataattg tcctctgaaa aatgaagttc ttgagtaccc
     3721 gaaagaggag tagcaaaagc tattggcccc ccgagcaaca gggatgcacc aacaacaaaa
     3781 ggagaaaaag acaatttgca tgttttttcc tttaccgtag gtaattctcc ggcccccccc
     3841 tgtgaacatt gggtcatatt acaaaaaaat ttgtgaattt gattcatgca ctatattatc
     3901 agtaagtggt tgataaaccc ctgaaaagat tccgttttat ccggaataaa gggacgatat
     3961 atgcaaaaac atattaaaca aagcctcaac caaaacatga gagctgtgtt atatatcaat
     4021 gcaacgggag aaatttacct gtgtcttgct ttgaaaaaaa gagggggaaa gctatatgtt
     4081 cgataataaa ttaataattt tactatgata gtctgcttca gaccgcaact tttcagttct
     4141 ttctgtacgc ttgcccaaag tttaaccact ttgtttttct atacacccaa tggttctatc
     4201 tgccccttga tgttaatccc caaagccaat aacatgaatg tattcatatc acaactttcc
     4261 taccagactc tccaatgcaa taactgcata attgttttta gtggaaatgt atcgttttct
     4321 taaagagaag aataacattc cattttatta tgttcccaat ttaattatat taatatatat
     4381 tggcattaat aggaaaatac atcaggagaa atcaaatgca gacatcaaac ataactaacc
     4441 atgaaagaaa tgactcatca tggatgtcaa ctgtcaaatc aactacagaa gtaagttgga
     4501 ataaattgag tttttgtgac attttactga aaataataac tttcggcata tattctccac
     4561 acgaaactct agccgaaaaa catagcgaaa aaaaactaat ggatagtttc tcaccatcac
     4621 tatcacaaga taaaatggac ggagagtttg cccacgcaaa catagatggc atttcaatac
     4681 ggctatgcct taacaaaggc atatgttcgg ttttttatct tgatggagat aaaatacaat
     4741 ccactcaact ttcatcaaaa gaatataata acttgttaag ttcactcccc cccaaacaat
     4801 tcaacttagg aaaagtacat actattacag ctccggtatc agggaatttt aaaactcaca
     4861 agcctgcacc agaagttatt gaaacagcca ttaattgctg tacatcgata ataccaaacg
     4921 atgattactt ccacgttaaa gacactgatt ttaatagtgt ttggcatgat atttacagag
     4981 atattcgagc atctgacagc aatagtacaa aaatatattt caacaatatt gaaataccac
     5041 taaaattaat tgcagatcta attaatgagt taggaattaa tgaattcatt gatagcaaaa
     5101 aagagcttca aatgctaagt tacaaccaag taaataaaat aatcaattca aacttccccc
     5161 aacaagacct atgttttcaa acagaaaaac ttttgtttac ttcactcttt caagaccctg
     5221 catttatatc tgcactaaca tctgcattct ggcaatctct tcacatcacg tcttcctctg
     5281 tggaacacat atatgcccaa attatgtccg aaaacataga aaacaggctt aatttcatgc
     5341 ctgaacaacg agttattaac aattgtggac atattataaa aatcaacgca gttgtcccta
     5401 aaaacgacac tgcaatctct gcctctgggg ggagggctta cgaagttagc tcatcaatat
     5461 taccatcgca cataacatgt aatggtgttg gtattaataa aatagaaaca agctatctcg
     5521 ttcatgctgg aacacttcca agcagtgaag ggcttcgcaa tgctatccct ccagaaagtc
     5581 gtcaagtatc atttgccatc atatctcctg atgtttaagc caatattata ttggctgcca
     5641 gactgtaaaa aaacgttctt cagaacagct ctataaacta ttaaaccaaa gctaaaagga
     5701 tggggaccaa tagaatctca gcccatcctt tttatatcat tatgaaccgc cccggaaatc
     5761 ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac acgtttttcc
     5821 cctgaagtcc gtcaacgggc agttcgtatg gttctggaaa gtcagggcga atatgactca
     5881 caatgggcag cgatttgttc cattgcccca aagattggct gtatactgga aactctgcgt
     5941 gtctgggtac gccagcatga gcgggatacc gggggcggtg aggtgggctc accaccgctg
     6001 aacgtcagcg tctgaaagag ccggaacgtg aaaatcgtga actgcgccgc agtaacaata
     6061 tccttcgcct ggcttccgct tattttgcaa aggcggagtt cga
//
LOCUS       DAJTQQ010000164         6086 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000164 DAJTQQ010000000
VERSION     DAJTQQ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.165"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            572..1750
                     /gene="ltrA"
                     /locus_tag="OO309_003879"
     CDS             572..1750
                     /gene="ltrA"
                     /locus_tag="OO309_003879"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5848832.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     NGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1833..2012
                     /locus_tag="OO309_003880"
                     /pseudo
     CDS             1833..2012
                     /locus_tag="OO309_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2042..2524)
                     /locus_tag="OO309_003881"
                     /pseudo
     CDS             complement(2042..2524)
                     /locus_tag="OO309_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2780..3760)
                     /locus_tag="OO309_003882"
     CDS             complement(2780..3760)
                     /locus_tag="OO309_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR5848833.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3760..4959)
                     /locus_tag="OO309_003883"
     CDS             complement(3760..4959)
                     /locus_tag="OO309_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5848834.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5281..5619)
                     /locus_tag="OO309_003884"
                     /pseudo
     CDS             complement(5281..5619)
                     /locus_tag="OO309_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5686..>6086)
                     /gene="istB"
                     /locus_tag="OO309_003885"
     CDS             complement(5686..>6086)
                     /gene="istB"
                     /locus_tag="OO309_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCR5848835.1"
                     /translation="RVGIKVRFTTAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIID
                     EIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRIL
                     HHSHVVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT         1656 a         1394 c         1445 g         1591 t
ORIGIN      
        1 ggctgtgggt ctacgttcgt gatgaccgta acgccggttc ggaaattgca gcataagtta
       61 tccatgctct gaggtgggta gaggtgggcc ctccggtcag ggtttacagg agcacagacc
      121 aaacgacccg gtgctgcttc attctaagga atgaggtggt gagcgactcg ggaaaaggca
      181 gagatctact gtcacgtggg ttcgggaagc tgaacgaaag taaactgtcc gatgaagcat
      241 cgaaatctta gatgatgcca aaatggggcc gtacagccgt cccctgataa gcgcagaaga
      301 cacctgctta ctgactgcgc ggcatccggt gtgaaggcag caggatcccg ggccagacca
      361 tctcagggaa cgtgagaacc tgtttccggc acgtatcggg aaacaggggc ggacccattc
      421 gtagtagtga tgaaagatct gtaatgggtc tggagctaag ggatggggat cacattcatt
      481 gcagaagaca caacccaaaa ggggatgacg taactgatgc aacgaaaatc atttgaaata
      541 ccaaaagcac tggtctgggc atcatatctg gatgtgcgtc gcaacaaggg agccccccgg
      601 atgcgacggg cagactttga aaatgttcga ccagcaacgt gatggcaatc tatacaagat
      661 ctggaaccgg ctatgttcgg gaacatggtt tccgccacca gtgctggaaa aacggatccc
      721 gaagtcgaat ggcaaggaac gcattctggg gatcccaaca gtatccgatc gaattgctca
      781 gggagcgata aaacttttca tggaagaaaa gcttgacccg attttccatg ctgattcata
      841 tggttaccga ccaggcaaat cagctcatga cgctctgaaa caatgtgcca tccggtgctg
      901 gcgttatagc tggatactgg aagtggacat cagtgcgttt ttcgaccatg tgagacatga
      961 tctggtgctc aaagctctgg aacaccacgg gatgcctaaa tgggtcatcc tgtactgtcg
     1021 acgctggatg gaagcgccaa tgcagagttg tgaaaatgga gaattaataa cccgaacacg
     1081 aggcactccg cagggcgggg tcattagtcc gctacttgct aacttgttcc ttcactatgc
     1141 atttgatttg tggatggaaa gagaatatcg gggggtaccg tttgagaggt acgctgacga
     1201 tattgtagta cattgttcac gaatgagtga tgcaacaagg ctaaagaaca ggttatcgga
     1261 gcgcttctcg gaagtcgggc tggtcctgaa tgcagggaaa acgaatatcg cctacattga
     1321 cacgtttaaa aggagaaacg tcgcaacgag tttcaccttt ctcggatatg acttcaaagt
     1381 gcgtacgctg aagaatttca acggcgaacg gtaccgaaaa tgcatgccgg gtgcgtcaaa
     1441 tgcagcaatg cgcaaaataa cagaaacaat caagaagtgg cgtatacatc gctcaacagc
     1501 tgagagtttg ctggattttg cgaggcgcta caatgcgata gtgagaggct ggatcgagta
     1561 ctacggaaag ttctggtcca gaaatttcaa ctatcgactg tggagtgcaa tgcagtcacg
     1621 tctgctcaag tggatgcagt ctaaatacag actttcgaac cggaaggctc agcgaaagct
     1681 gacgctggta aggaaggagt atccgaagct atttgttcac tggtatttac tacgtgcatc
     1741 aaatgagtga tcaagagccg tatgacgcga gagtgtcacg tacggttccg tgagcagccg
     1801 gggagtgaaa ttctctccgg ctgactcgac tcgcgggttc cgacagcgat gacgaacatg
     1861 cggcggtgtt gtactcgctg atcggcacat gccgtctgaa caatgtggaa ccagaaaaat
     1921 ggctgcgtta cgtcattgag catatccagg actggccggc aaaccgggta cgtgatctgt
     1981 tgccctggaa agttgatctg agctctcagt aaatatcaat acggttctga cgagccgctt
     2041 acttttggcg taattctgct gcgcctttac tgaacacaat gccgtattca gcaagaagtc
     2101 cacgaatgtg atttatcagt tgtgtccttt ccattgaaac caaccgacgc atattcaaaa
     2161 aagtctggac gtcctgttgc tccagagttt ttattggtac gggtcgtata gttccatgtt
     2221 ggcaggcctc agctattgcc tgtgcgtcgt tataatcatt tttctggcac cgctgataag
     2281 ctttaacatg ttgtgccgga agcaatatta catcatggtc gagtttcctg atttcccggg
     2341 cccagtaatg agatgccccg ccagcctcca tcgcaacagt cattttaggc tgttgcacca
     2401 gccagttaag cagttgattg cgagaaagtt tggtctttcc tgcgggtttg ccatgagcat
     2461 tgatactgaa aagataaaag ttcgttttag ccagatcaat gccggtgatt ttctgttcag
     2521 acatggtatc ccgcttactt ttgttgttaa agtttatcca tcatggcgca attgaaggct
     2581 gccgtgaaag agggggaaga ctatctcatc ggttactgtt aaacggctaa gagaaactat
     2641 ctttttcaag caaagaagat acggtgaaac aggcgatttc accgtgaaat actgttcaaa
     2701 aaaaacgcta ataaaagcaa aatttctttt aaaacagcca attaaaaact ggcgagttgc
     2761 tggcgaaaaa cttgaatttt cacagcgaat tcagattatc ttttagtaca agagcaatca
     2821 tacgatctaa cttatcctga acttccaacg gtaatctgcc gaattcatat gagaacgtac
     2881 gtccctttac tcgttttcga gcaaatttat ctttactatc aaatttccat aacaaagtta
     2941 caaccgcttt atcctttact cctgaatcaa gcaatgaaga catttccttg gttataagcc
     3001 gcataatact gtttttaacc tcgtcttcag aaagattgtc atcagcagta ataagctcaa
     3061 tactcggcga gatattcttc acaacttcat caatagatat atcacccagc ctcagtcgtt
     3121 cacttaattc agccagttgc ctgtaatcgg cgtatgtcag ctcagactga acaggaaaaa
     3181 gagacacaaa atctttaggt acggacgctg cttggagggc acgagtcacc ttagctgccg
     3241 acaatccctc tctttccgca atttgcttct ggttcatacc ggcctctttg agacgtacta
     3301 aacgcaaccc aatttcacga atattatgtt caagtgacgt ctgcaaatct ttcgccagat
     3361 gctgagcttc agaaacagta agctcatcat ccgttacaag tacacgtaat ccaaccttgc
     3421 atagcaaagc tgctgcccga cgacgggaac catccaaaat ctcaataaga tcgccagtcc
     3481 gaatcccaat acatggatag aattgttgta aacgtatagt tctggtgatg tcttttagtg
     3541 agtccggcgt cagggctgtc tgttcgcggc cattcacctc ttcaaccaca aaagtttgag
     3601 tatccacttt gtcatgatca accttgatct cagtaaattt tgcctttcgg ccagtattga
     3661 gggtaaatac atgaactgga gccgatattt cttcagtatt attcaggata tttgtattca
     3721 gcgtacggcc aatagtcggt cggtgctttc tgttttccat cagtagttgg cccttataaa
     3781 ttcaatacga tcaaaaactg ctttagcgaa atcttctgct gccatacgtg catttttcaa
     3841 agcttcacca ctgccaacat aagtaacagg attcgcagat atcacggtat caaacgactc
     3901 gccgcttctc tcaaaaccat caagacgtgg cattgaaaca tcaagcatgt caccaccgaa
     3961 aatctctttt gtcaggctat ggcagtattt atgatctgac ttattcgcca gtttcgacat
     4021 gaagccaata tttgcctgca aacggcatga acatccatcc tgttcaataa tctgtacaag
     4081 ttcaggtaaa cgagccaaat atttaagcgt ggaatggaaa tcaacttgtg ctggcggcac
     4141 cggtgtaaac atgatatctg cggcagcaat ggcatttttc aaaaatgcgt ccagatgcgg
     4201 gcctgtatca ataaggatga agtcgaaatc atgcttcagc ttatcaataa tattctcacg
     4261 caatatcgcg tgtttgtttt gtcctaatag atgctcctcg cacagagtat cccagtttga
     4321 tgcaataaag gcgtcgtcaa tagaagccgg cataacataa acgccaggga taactgacgg
     4381 aacgataaaa tcttccagta attcctcacg actcacgttc tgcaacatag cctgcggtgc
     4441 ggttgtatca accagcccca ccgcatgcaa gtagttcaga aacatagtgg ctgatgactg
     4501 gggatctaaa tcaaggagca gaatacgtaa atcttctgac agcaggtgag gatgagcacg
     4561 cagggcgtga gctacggaaa cactactcac ggttttagac acaccgcctt ttagactacc
     4621 gatgaatatg gaatatgcct cactataacg atcgcgatat ttaggaatgc cacgatgtgc
     4681 atagatatcg ataatgttct ggatggttaa tgcatatttt gtcgcggttc ctgctgggcg
     4741 tttttcaaac tgatagccct gagcctccat ctcaccgact gcaccatcca cgcttgcgcg
     4801 cgttaatttt ggaagcttag ctacagcaga ctttgaatag acttgataaa aaacatcagc
     4861 ctgaaactcc tgcttttgct cctgaatctg ttttgttagg gcgagcaaca ttttatctgc
     4921 acgctgcgca accttactta actgctcaaa actagtcatt ccccctccgt gctatgtagg
     4981 attttaactt attacaaaaa aaactgcatt agcagggtaa tgcatgcaag tacatattgt
     5041 acatgcttat attaaaaatg cgcgcattgc tgatttatta atcatcggaa tgaatattac
     5101 tgtaagatag tatgcctttc cgggagaaag acgctgattt cccccgaata atccagttca
     5161 ccaccacact ccggacaaca gctttcttca ggctccagat ggtgtatttc gcgggaaaca
     5221 tgttccggta acggacggcg atgacgtgac tgtcgcaaca ggcgggggta gtgcttccaa
     5281 tcagtagaac atgtgctttt caatgaagga gccgatgact ttttcgtgga tttccacgga
     5341 acgcgagaag cagattgttt tacgtgccag ccgcttgatg cgggtgcgga gggtccggtt
     5401 gttacgtgca atacgttgcg tgaagatttt gccggtcagg tgtttctcct tcggcacttc
     5461 tctggcgtag ctgccccagt catcgctggt caccatgccg atacagaacg gagtcaacag
     5521 ggctaacagt tcccgacagg tctcatcatt ccgtggcccg aaagtgtaag ccaacacacc
     5581 gccagtttta gtgttgtacg cataccagag ccagtgttgt gtcaacgacg gatgaaaagt
     5641 gatccactta tatctccacc aacggcccaa tattgatcca ccgttttact caggattagc
     5701 ttctgctata accccggcct ttcgtttctg tctgagtcga tagctttctc ctttgatttg
     5761 aacgacatgt gagtggtgta agatacggcc cagcatcgct gaggtcagtg ctgcatcacc
     5821 ggcgaacgtt tgatcccact gcccgaacgg tagattggat gtcaggatca ttgcgctctt
     5881 ttcgtaacgt ttagcgatga cctggaagaa cagttttgct tcttcctgac tgaacggcag
     5941 atagcctatt tcatcaatga tgagcaggcg gggggccatt actccacgct gaagcgtcgt
     6001 tttataacgg ccctgacgtt gtgccgtaga taactgaagt aacagatctg ctgctgttgt
     6061 gaagcgaact ttgataccta cacgga
//
LOCUS       DAJTQQ010000165         6025 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000165 DAJTQQ010000000
VERSION     DAJTQQ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.166"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OO309_003886"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OO309_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR5848836.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OO309_003887"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OO309_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR5848837.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OO309_003888"
                     /pseudo
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="OO309_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(5797..>6025)
                     /locus_tag="OO309_003889"
     CDS             complement(5797..>6025)
                     /locus_tag="OO309_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HCR5848838.1"
                     /translation="HNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQAGDR
                     VIVSGLQRIRPGIKARAISSSQENASTESKQ"
BASE COUNT         1578 a         1421 c         1429 g         1597 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt acaggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcaacagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg ccccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacgacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgg
//
LOCUS       DAJTQQ010000166         5795 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000166 DAJTQQ010000000
VERSION     DAJTQQ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.168"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(226..690)
                     /locus_tag="OO309_003890"
     CDS             complement(226..690)
                     /locus_tag="OO309_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5848839.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(687..1562)
                     /gene="nanK"
                     /locus_tag="OO309_003891"
     CDS             complement(687..1562)
                     /gene="nanK"
                     /locus_tag="OO309_003891"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR5848840.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1559..2248)
                     /locus_tag="OO309_003892"
     CDS             complement(1559..2248)
                     /locus_tag="OO309_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR5848841.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2296..3786)
                     /gene="nanT"
                     /locus_tag="OO309_003893"
     CDS             complement(2296..3786)
                     /gene="nanT"
                     /locus_tag="OO309_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR5848842.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(3896..4789)
                     /gene="nanA"
                     /locus_tag="OO309_003894"
     CDS             complement(3896..4789)
                     /gene="nanA"
                     /locus_tag="OO309_003894"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR5848843.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(4911..5702)
                     /gene="nanR"
                     /locus_tag="OO309_003895"
     CDS             complement(4911..5702)
                     /gene="nanR"
                     /locus_tag="OO309_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR5848844.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFDEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1416 a         1608 c         1486 g         1285 t
ORIGIN      
        1 gaagcaattc ggctggtgat tgatgaacgt cattccattc gctgcagtac acttacagca
       61 actattacga ataaaaaggg caagcgaatt aagcggacag gtatatacag tatcaagggt
      121 gttaacataa aagaatacaa cgtcaggtaa tggcacagag cggtccgtca gttcaggttt
      181 ggctctgtgc caaagatggg tcaggtcaca cctgatctaa taaatttaag ccattaaatc
      241 agccttaacc ttcaccacca cctttttaat ctcacccggc tcgccaacaa cgcatcccgg
      301 cttatgcggc tctcctggca taaacacggc gaacattccc ggtttcagaa tgatggtttg
      361 ctcgttttca atggcgctac aaagctggta atcatcttca tggtggaact cttcacattg
      421 gcgcgcagtg cctgccatgc caaacagaat ccgttcctca ccgtttaata acagctggat
      481 atcaatgtat tgctcgtgca attccgcttt tttctcgacg ggcgattgag tgttaaacgt
      541 catgacattc ataaaaatat tgtcgccctg taattcgtaa cgacccggcg ctttttcttg
      601 cggtctggca gctaaggcca gcgttaacgc gtcctgtaac gcaggatgta acccagcaga
      661 cggtaatgac tgtacttcac ccatcatcat aatttttctc cctgggccaa cagcgcagcc
      721 ccaagtaaac ctgcatcatg gcggtaatgc gccgccagta aatcaacatg aaatgccgct
      781 ggctcctgcg ccagatacat ttccaccaac gccagatacc cttctgccag accaacgctg
      841 ccaccaacca ccacgcactg gcaatcagtt gtggctttaa tatcagcgat cagccttgca
      901 agcgtacgtg cggagcgatg aatcagttgc tgcgcctgct catcgccctg cccggcgcgt
      961 gtgaaaatag ttctcgcatc cgcgccagcc agctcgccct gcgctgccgc cgcaatgccg
     1021 cgaccagaag caattgcttc cacgcaacct gtgcgtccac agccgcagac tgggccgtgt
     1081 ggatcggcaa gcgtatgccc gatgtgcccc gccagaccgc cagggccggt aagcagtttg
     1141 ccgccgctca ctacaccgcc gccgacgcca gtggaaacgg tgataaagac catatcggtt
     1201 atatcgcctt ccagcgcctg atactctgcc catgctgcgg cctgcgcgtc gttaatggca
     1261 atggtcggca aattggtaag ttgttccagc gttttgacta acggaaagtg tagcaatcca
     1321 ccgagattat gcggattcag cgccagcaaa ctgccgtcgc gaataattcc ggttgaagcg
     1381 atggcaaccc gctgcgcatg agcttgcaac ggagagacta atgcggataa ggcatcacgc
     1441 aaggcttctg gtgtctggct ggctggcgta ggaagttcac gacgatcgcg gatctgcccg
     1501 tcagcgccaa tcagcgcggc ggcaagttta gtaccgccga tatcaatcgc cagtatggtc
     1561 atagcaccgc ctttttcatc gctgtgttgt accactgaca aatgtgttca agacgcgtaa
     1621 tggcagaacc gaccgtcacc gcccacgcgc cgtggcgcat cgcatctgcc gcctgagcag
     1681 gcgtgttgta acgcccttcg gcaatcaccc gacatccggc ttcgctcaac gttttcacca
     1741 gcgccagatc cggctcttct ggcgtttcag gcgtggtata gccagaaagc gtagtgccaa
     1801 taatttcggc tcccagcttt tggcatgcca ggccgtcttc cggcgttgag cagtcggtca
     1861 tcgccagtaa accgtgatga tggatacgtg ccagcagcgt ttcaacaggc accggacgcg
     1921 ggcggtcggt gccgtcaatg gcgataatgt ccgcgcccgc ctgcgccagc gcatcgacat
     1981 cttcaatata ggccgtgatg cgtaccggag aatcctccag atcgcgtttc acaattccaa
     2041 taatcggcac gctcaccacc gcacgcgtgg cttgcagatt tgccacacct tcgatgcgaa
     2101 tggcaaccgc gcccgcctgc tctgccgcta atgccatggc tgcgacgatt tcgggtttat
     2161 cgagcgggct gtcaggaacc ggctggcagg agacaatcag gccaccgtta gcagcgattt
     2221 tttgatccag ttgtgcaagt aacgacatac atcttccctt agcgaaaggc ccggtacata
     2281 gaccgggcaa caggattaac ttttggtttt gactaaatcg tttttggcgc tgccaaatgg
     2341 cacggcaccg ctaaatggtt tgccgtcgat agcgtcatga gtacgcaatg cttccgggcg
     2401 cagccaacgc tgaacgcggg aaggcatatc cagaccaatc agcaggatca ccacaaatgt
     2461 caggctgaac gagagcgatg ccagcgcagt acccagatcc agacgttgag cgatcaacgc
     2521 gccgatgatt ggggccagtg cgccacccaa tgcgccaacg ttgtaggtaa agcccaggcc
     2581 cgctgcacgc tggtcggtat cgaaataacc gccaatcagt ttcggtaaga tcccggcgat
     2641 cccttgtcca agcatttgct ggaagaacag taacagaccg agcacccaga cgtttgcgcc
     2701 gccaatcgca aataccggaa taatcagcag ctgcgaggcc agcaggctac aaacgtacgc
     2761 tttgcgggtt cccagccagt caccgaggaa gccacctacg cagcatccca cggccgcgcc
     2821 aaagccacta aagaacagca cattggcgac agtatgcggg ttataagcca gatcggtttt
     2881 cagatacgtt ggcagcagcg cctgaatcgg ccatgagtag aggaaagcaa acaacacgac
     2941 caccatcagc attacgcccg ttggccagcg tttacccgta ctttgcacca taaagctgat
     3001 aaagattgcg gcgcataaca gcccgagaac agcgacgatt gcagcatttt gcaggttacc
     3061 ggcgaagcag aaccacagcg cagtagccgc cgccagtgtc attacgatat tggcaatgcg
     3121 atgttcacca cggtagagaa tatccaccat tgtgcgtact ggtgctttac ctgcgtgttt
     3181 ctctttccag tcttccgctt ccgggatgtt tttacgcagc cagagagcaa agatgattgg
     3241 caaaatgccg ataaagaaca gcgcacgcca accccagacc ggaaccacca ggctatagac
     3301 ctgagcggca acgacggccc ccacagagaa gcctgaaatc aaaaaaccac tggctttgtt
     3361 acgcagatgt tttggccagc tttcaatgac ataggtggcg ctggaaccgt attcacccgc
     3421 catccccatg ccgatgacca gacgagcgat aaacatggtg atgtagcctg gcgcaaagcc
     3481 gcaggccagc gtcccggccg agaagagaac gatgctggtg accattgcca gacgacgccc
     3541 gtagcggtca cccatagcgc cgagcatcag gccgccgaac cagcgagaga taaaggctgc
     3601 ggagatcaga cttgccgcct gcaccgtcgt cagcccgaat tcaccctgta cttcggtgag
     3661 taccagggcg attaaaacga aatcaaaacc gtcaagcaga tatcccaacc aggcagcgga
     3721 aaatgcgcgc cattgtgcac ggttgagatg gcgataccac gggatattct gggttgtagt
     3781 actcattgtg agtctcccgc ggtgggcgat gcccacacgc tttggtatga aaattgtagg
     3841 gtacagatgc gtttatttcc cctcacccgg taggggcgag cgaggggaaa acaactcacc
     3901 cgcgctcttg catcaactgc tgggccagcg ccttcagttc tggcagatat ttttcatcta
     3961 ccggtccaaa cggtttgcgg cacagcggca cagaaacgac atccatataa tgcaggacag
     4021 ttttcaggcc gcggaatacg cccgttttga tcagtaaatc aatgactttg ttgcactcag
     4081 tttgcagttt ctgcgcggtc tggatatcgc cttctttcag cgccttcacg atcccctgat
     4141 atcgccagcc catgatgttg taagtactgc cgataccacc atcagcgcct gccagcaagc
     4201 cggaagcgaa gatttcgtcg taaccgttat agagcaccag atcaggatgt tcacgacgga
     4261 tctgttccat ctgatagaga tcgccagaag tctgtttcag cgcaccgacg ccaggcaacg
     4321 taaccagggt gttgatctga tccagggtca gttttacccc actcagggct ggaatgttgt
     4381 acaccaccat cggcaaacca tccgctgaat caataattgc ccgatagtga tcgcagtgtt
     4441 cttcaaagct gaaaggatag tagaacggcg tgacggcgga gacggcatcg aagccataac
     4501 gtttagccga tgccgcaagt tgttggcttt cggcggtgct gacgcaaccg acgtgggcga
     4561 tgagtttaat cttacctttc gcctcttcgg cgacgatttc cagtacctgt tcacgctcgg
     4621 aaaggctttg tacaaaggct tcgccggtcg aaccacccac gtataaaccg tcgatgccct
     4681 gctgaatatt gaactgaacc aggcggcgca gactcgcttt atccagtgct tgttgttgat
     4741 caaaaggagt caggagtgca gccattacgc cacgtaaatt cgttgccata aatacctctg
     4801 aagtgatgct tgtctgataa acgatatacc tttatacctg ttataccaga tcaattaagc
     4861 aacaccccat acagaaagct tataatgcga tctgcttcac taaagtggca ttatttcttt
     4921 ttgttggtgg tctgaccgaa agcgtgccag gtagcagaga cgctgttgag atgcgattgc
     4981 aacgcacgat cggcttcgtc aggatcatga cggcggatcg catcaacgat cgcaatatgc
     5041 tgttgataac taacgttgtt atgttcgtgc agtgcctgat cggtaaccgt tgggcgtgcg
     5101 gcaataagcc agtcgagcag ggcaacgtgg atcgccatga agattgggtt accggggatc
     5161 tccgccagca cgcggtggaa atcaacgtct gaacgaatga atgccgcgtt gttatccagc
     5221 gactgactgt tgatttccag tgcttttgcc agcaaatcga tttgctcatc ggtggcgtgt
     5281 tcagccgcat agcgcaccag gctggattca aagaacagac gtaattgttc gaaatgggca
     5341 atcccaccag gatgagaaag gaaatctttc gccatgccgg aaagctcgcc gatgatagtg
     5401 tccgcagaag gtcgagagac acgagcgcgt tcgccgttgt ttatttgcac cagacctttg
     5461 cgttttaacg ctgccagcgc ttcacgcaca gaaggacgcc cgacgttaaa gaacgccatc
     5521 agttcgcgtt cagacggtaa ttgttcacct tcgtcaaatt cacgacggcg gatcatctgt
     5581 tccagctctt cttccaccat ttcggagagt tttttacgcg ccagcgggcg gctacgcaag
     5641 ttgcgaccaa ttgcaggtga agaatcttcg gtttgcgaat caaatgcgtt cataaggccc
     5701 attctgtaag gtcagtgtga ttaacatcat cagtgacatc ctatcacagg attgaaagta
     5761 ggggaaaatg gcaggttttt ctctttgtgc ctcat
//
LOCUS       DAJTQQ010000167         5730 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000167 DAJTQQ010000000
VERSION     DAJTQQ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.169"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..231
                     /locus_tag="OO309_003896"
     CDS             <1..231
                     /locus_tag="OO309_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848845.1"
                     /translation="FYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPRTVG
                     KREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(228..1169)
                     /locus_tag="OO309_003897"
     CDS             complement(228..1169)
                     /locus_tag="OO309_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCR5848846.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1181..1670)
                     /locus_tag="OO309_003898"
                     /pseudo
     CDS             complement(1181..1670)
                     /locus_tag="OO309_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1674..2894)
                     /locus_tag="OO309_003899"
     CDS             complement(1674..2894)
                     /locus_tag="OO309_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR5848847.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2898..3641)
                     /locus_tag="OO309_003900"
     CDS             complement(2898..3641)
                     /locus_tag="OO309_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR5848848.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3532..4998)
                     /locus_tag="OO309_003901"
     CDS             complement(3532..4998)
                     /locus_tag="OO309_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCR5848849.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(4998..5231)
                     /locus_tag="OO309_003902"
     CDS             complement(4998..5231)
                     /locus_tag="OO309_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR5848850.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            complement(5219..5698)
                     /locus_tag="OO309_003903"
     CDS             complement(5219..5698)
                     /locus_tag="OO309_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848851.1"
                     /translation="MDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEIRLTPLTTENSILWMS"
BASE COUNT         1225 a         1616 c         1434 g         1455 t
ORIGIN      
        1 ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt gcgcacgagt
       61 gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa actcgcgccg
      121 agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg catcattttt
      181 tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta aatgccatca
      241 acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc gtagtaagtg
      301 acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac cagcgggtag
      361 cggacgtatt ttttatcgtt ggtgtacgca actatgcgat ccttattccc cacaccacgg
      421 cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg atagctgatg
      481 gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac gatgatgctg
      541 gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac agagtaacca
      601 gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc gtccggtgtt
      661 gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt aacaaggcct
      721 ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat ctgccatttc
      781 agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg agcagcctgc
      841 aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt accggatttg
      901 ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca gttttttcca
      961 ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct ggaaatgtca
     1021 tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta ccccaccagt
     1081 ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc accagagctg
     1141 tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc caggaaattt
     1201 ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc accacgtttt
     1261 tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat tcatcagtgg
     1321 agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac ggtcacgtag
     1381 ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg aaccatgtcc
     1441 ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc tgcggtatca
     1501 ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag gccataggca
     1561 gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg cgggcgagag
     1621 acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat tttttaccct
     1681 ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact ggtcatgccc
     1741 atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt ggcaacctca
     1801 gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat atccgcatca
     1861 ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg tttgaacgcg
     1921 gtcggtttca tcttacggct cagatcgata cccggaacga taacttcggc acgccaggct
     1981 gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt cggattatcg
     2041 tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag cagggctttc
     2101 agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat ctctggtgct
     2161 ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc ctgcggcaaa
     2221 tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt catggtgtcg
     2281 gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt ttgatttgcc
     2341 attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg gacaagagcc
     2401 acatggtttc cggtaatatc gacctgctcg gctttacctg gctcggtctg ctcgtactcc
     2461 gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat ggcgctttcg
     2521 tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc gcgccggaca
     2581 ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag ccgcacattc
     2641 ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga ggcaagcgtg
     2701 gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa cttatcgggt
     2761 ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc tatcggcacg
     2821 tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag cctggtctca
     2881 aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg aacaaacacg
     2941 atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat gccgtccaaa
     3001 gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga atctcggaca
     3061 tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat cgctcgggcc
     3121 atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc ggccctagac
     3181 ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg accaccggtg
     3241 acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc ctcgagaggc
     3301 agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg gcccacagga
     3361 gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg ccgccaaact
     3421 ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt cgcccagggg
     3481 gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact gtcatttgaa
     3541 ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg taggctggtt
     3601 cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc cgatgccggg
     3661 cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc ctatgccggt
     3721 tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca gaccatcacc
     3781 cagcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat cgacatcaga
     3841 catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct tgccaagttc
     3901 cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc tctcctgcaa
     3961 cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa atcctttcgg
     4021 ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct gttctgcaaa
     4081 ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa acttatcccg
     4141 cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt cgatattctt
     4201 cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct tctccacgct
     4261 gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag cgctgtcgaa
     4321 cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt tctcggtcat
     4381 cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt gatgaatgcg
     4441 ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt tacccaggtc
     4501 cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc ggtcgagcgg
     4561 gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg tcattggtgc
     4621 ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc atttcagcac
     4681 gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa tgccacgatc
     4741 ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat ccgggatagc
     4801 atcgactgct tcgccgatga tccaggatga acgataggac cattccacca gcatgcggtt
     4861 acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg tctgcatccc
     4921 tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc gccgcagggc
     4981 ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc cagcgatgta
     5041 tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg tatctcgcgt
     5101 tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa atcacgatga
     5161 ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc tatgctgatc
     5221 agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt tctgaaacgg
     5281 tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg atgccgttgt
     5341 actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa ttcacacgtc
     5401 gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta gcaccggcag
     5461 acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctccagc gccgcctgat
     5521 aagtacgctg gcagctcttc agtaattcat cttctttggc cttccattcg tcggcccaga
     5581 aaacaacgga tccgtgacgg taaacgttag cgcacttatc tgtaccactg tcagccacgt
     5641 caaagccaat acgctttctt ccactgggtt cgaaatttaa cgttttgtgc gcatccactg
     5701 cggcttctat ccaagacagt ttgatgattg
//
LOCUS       DAJTQQ010000168         5693 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000168 DAJTQQ010000000
VERSION     DAJTQQ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.170"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..171
                     /locus_tag="OO309_003904"
     CDS             <1..171
                     /locus_tag="OO309_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5848852.1"
                     /translation="IRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP
                     KRYRKKLTEFNQ"
     gene            276..1628
                     /gene="celF"
                     /locus_tag="OO309_003905"
                     /gene_synonym="chbF"
     CDS             276..1628
                     /gene="celF"
                     /locus_tag="OO309_003905"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR5848853.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1641..2399
                     /gene="chbG"
                     /locus_tag="OO309_003906"
     CDS             1641..2399
                     /gene="chbG"
                     /locus_tag="OO309_003906"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR5848854.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2446..4707)
                     /gene="katE"
                     /locus_tag="OO309_003907"
     CDS             complement(2446..4707)
                     /gene="katE"
                     /locus_tag="OO309_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR5848855.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4890..5153
                     /gene="cedA"
                     /locus_tag="OO309_003908"
     CDS             4890..5153
                     /gene="cedA"
                     /locus_tag="OO309_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR5848856.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5430..>5693
                     /locus_tag="OO309_003909"
     CDS             5430..>5693
                     /locus_tag="OO309_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848857.1"
                     /translation="MRREMFSTNLIQSNYGDLNIKSLAFDSFKERLESTMAALTFFIS
                     TGQCDCDEIAESNFNRMIAYMSNINYDASKLGAPALSFDTYLQD"
BASE COUNT         1426 a         1247 c         1468 g         1552 t
ORIGIN      
        1 atccgtatta attttgcaaa aaaacaactg gaaatgacca actattccgt gacggatatt
       61 gcgtttgagg ccggttatag tagcccgagt ttgtttatta aaacgtttaa gaaattaacg
      121 tcctttacgc ctaagagata tcgtaagaaa ttgactgaat ttaatcagta agtcgttata
      181 cctgacaatt cacatatctg tcctgttgct ggattattta tgtccggggg ggcagatatg
      241 ccagatatca gtattctgta ctgaagggag aaattatgag ccagaaatta aaagtcgtca
      301 ctattggtgg cgggagcagc tataccccgg agttactgga aggatttatt aagcgttatc
      361 acgaattgcc ggtcagcgaa ttatggctgg tggatgtcga gggtggcaaa gctaaactgg
      421 atattatttt cgatctctgc caacggatga ttgataacgc tggcgtcccg atgaagcttt
      481 ataaaacgct ggattgccgc gaagcattga aagatgctga tttcgttact acccaactgc
      541 gcgttggcca attaccggcg cgtgaactgg atgaacgtat tccattaagt catggttatc
      601 ttggtcagga aaccaacggc gcgggcggtt tgtttaaagg tctgcgtacc attccggtga
      661 tttttgacat cgtaaaagat gtcgaagaac tttgtccgaa tgcatgggtg attaacttca
      721 ctaacccggc gggaatggtt actgaagccg tttatcgtca taccggattt aaacgcttta
      781 tcggcgtgtg taatattccg atcggcatga agatgtttat tcacgatgtt ctgatgctga
      841 aagacagcga tgatttatct atcgatttgt ttggcctcaa ccatatggtg ttcattaagg
      901 atgtgctggt aaatggcaag tcgcgctttg ccgaattgct tgatggtgtg gcatccgggc
      961 agttaaaagt atctggcgtt aaaaatattt tcgatctgcc atttagcgaa ggcttaattc
     1021 gttctctgaa tctgttgccg tgttcttatt tgctttatta cttcaagcaa aaagagatgc
     1081 tggctattga aatgggcgaa tactacaaag gcggcgcacg agcgcaggta gtacagaaag
     1141 tcgagaaaca actttttgag ctgtataaaa acccggagtt gaaagttaag ccgaaagaac
     1201 tggaacagcg cggtggggct tattactctg atgcagcgtg cgaagtgatc aacgctatct
     1261 acaacgacaa gcaagctgaa cattacgtta atatcccgca tcatgggcat attgataata
     1321 ttccggcaga ctgggcggta gaaatgacct gtaagctggg gcgcgatggc gcgacgccac
     1381 atccgcgcat tacgcatttc gatgataaag tgatggggct gattcacacc attaaaggct
     1441 tcgagattgc tgccagcaac gccgcactta gcggagaatt taacgatgtg ttactggcgc
     1501 taaaccttag tccgttggtg cattccgatc gcgatgctga gctgctggca cgcgagatga
     1561 ttctggcgca cgagaaatgg ctgccaaatt ttgccgactg catcgcagag cttaaaaaag
     1621 cacattaacc gaggctgatt atggaacgct tactgattgt taatgccgat gattttggct
     1681 taagcaaagg tcagaactac ggcattatcg aggcctgtcg caatgggatt gtcacatcga
     1741 cgacggcgct ggtgaatggg caggctattg accatgcggt gcagttgagt cgtgatgaac
     1801 cgagtctggc catagggatg cactttgtcc ttactatggg caagccactg acagctatgc
     1861 cggggttaac ccgcgatggt gtgctgggaa aatggatctg gcagttggca gaagaagatg
     1921 ctttaccgct ggaagaaatt actcaggagc ttgccagtca gtatttgcgt ttcattgagc
     1981 tatttggacg caaacctacg catcttgata gccatcatca tgtgcatatg ttcccgcaga
     2041 ttttcccgat tgttgccagg tttgcggctg aagaggggat tgcgttgcgc atcgaccgtc
     2101 agccgctatc taacgctggt gatttaccgg ctaatctgcg cagttcgcag ggattcagta
     2161 gcgcgtttta tggtgaagag attagcgagg cactgttcct gcaagtgctg gatgacgcaa
     2221 gccatcgggg tgatccttcg ctggaggtga tgtgtcatcc ggcgtttatc gataatacta
     2281 tccgtcagag tgcttactgt ttcccacggt taacggagct ggaagtgctg acatcagcgt
     2341 cgttgaaata tgcaattgct gagcgtggtt atcggttggg tagttatctt gatgtgtaag
     2401 taaggtggta aggtcactcc ccaggggacg tgaccttatc catatttacg ccggaatttt
     2461 gtcaatctta ggaatgcgtg accacacgcg gtgttctgcc atcagcgtta acagttcatc
     2521 cataaaacta ccgtcggtgc tgtcagcttc cacaatccct tcttcaccct ggtcagctac
     2581 cttgattgtt gctttaaact tgcgcgcgtc tcccgccagc gcaatcggtt taaggtgttt
     2641 gtaggcttcc atcagatagt agttggcatc gccgttgtca gcgatatccg cgatattgcc
     2701 gcaagggaca atgaccgcat cgaccgtcag cgaaggtgca ccggcaaagg tagcggctat
     2761 aggcaacacc gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga gcagtttggc
     2821 atgaacgcct ttggccttca tggccttgag aatggccaga aggtctgccg atctcacttc
     2881 atcattaagt aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa tggcgtacaa
     2941 acttaaggat ggatcctttt tcagaccgtt gacgtccgga ggtggggtga tattcagctg
     3001 gtcgtcagtc agttcgatac cgagattttt cgccaccgcc tgggccagag tgagatcaat
     3061 atgcgccagc tggtcaacaa cgcgctcacg aatatacgga cgaacgactt tgcttaactc
     3121 aaaactgaaa ccatcgacaa tatggcgctg ctcgaatggc gtctgactta gccagaacag
     3181 acgcggatgg gaataatatt cgccaaacga tgggctgcgc tcgcgaactt tattgccttc
     3241 cacgcgctct tggtatgatt caaaaccgcc gcgtttcggc cccggtggtg tttcgcgcgg
     3301 ccagttatcg ttgatcgagt tcggttcata attcgccggg ttagtgtcga tccccatacg
     3361 atgcatgccg tcacgctgga aattatggta agggcaggtc gggcggttaa tcggaatctc
     3421 atggaaattc ggcccaccaa gacgactgat ttgtgtatcg gtataggaga acaaacgtcc
     3481 ctgcaacagg ggatcgttgg tgaagtccag accaggaaca atatgacctg gatggaatgc
     3541 tgcttgttcg ttttcggcaa agaagttatc cggattgcga ttgagcacca ttttgccgac
     3601 acgctgaacg ggcaccagtt cttccgggat cagtttggtt ggatcgagaa gatcgaagtc
     3661 gaacttgaat tcatcttctt caggaatcaa ctggaagccc agttcgtatt ccggaaaatc
     3721 gcctgcttca atggcttccc acaactcgcg gcggtggaag tccgggtcac gtccggtgag
     3781 tttttgtgct tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc agtggaaacg
     3841 tacaaacgtt gccttccctt cggcattaat caggcggaag gtgtgaatac cgaagccttc
     3901 catggtgcgg taactgcggg ggatgccgcg atccgacatc gcccacatca cgttgtgcag
     3961 agtttcaggt tgcagagaaa cataatccca gaaagtatcg tgggcacttt gcccttgtgg
     4021 aattgcccag tgcggttctg gttttaccgc atgaacaaaa tcggggaatt tatgcgcatc
     4081 ctggataaag aagattggcg tgttattgcc aacgaggtca aaaataccct cttcagtata
     4141 gaacttggtg gcaaaaccac ggatatcacg cacggtatcg gcagagccag cgccaccctg
     4201 aacggtagag aacctaacaa atactggggt gattttgttc ggatctgaga ggaaatccgc
     4261 tttggtgata tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg ctgagccgcg
     4321 tgcatgaaca atacgttccg ggatgcgctc atggtcaaag tgggtgattt tctcgcgcag
     4381 aataaaatct tccagcagcg ttggaccacg gttaccggca cgcagtgagt tttgatcatc
     4441 ggcgatgcgc acgccctgat tagtggtcag cgcataattt tcactgcctt tgcgtacgtc
     4501 ttccagagaa ttaagttttt cgttacgtgt atcaggggct ttcaggctcc caggggcggt
     4561 aggttgtgca cctggcggtg ttggttcagc cgctggacga tgagatccgt cctcaggtgc
     4621 cagtgagtcc atccccggtt tcgcttcgct ggaatcgtgt agtggtgact ggtgctgatg
     4681 tgggttcttt tcgttatgtt gcgacattga actcgtctcc ttaatttatt actgaaaggg
     4741 ccgctgtttt ggtcaaaaaa ctctctaact atagaccacc agccagatcc cgcttcggag
     4801 actattctgg aaacggtgtt atgaaataca cgcaaagcgg gacgtacagg ggctaaatcg
     4861 gctaaactac agtttttcta atttttaata tgcgtttagt gaagccagta atgaagaaac
     4921 cgctccgcca gcaaaaccgc cagattatta gctatgtccc acgcacggaa cccgcgccgc
     4981 cagaacatgc gataaagatg gattcgtttc gtgatgtctg gatgctgcgt ggcaaatatg
     5041 ttgcgtttgt actgatggga gagtcatttc tgcgctcacc ggcgtttact gtgcctgaat
     5101 ctgcccaacg ttgggcaaat cagatccgcc aggaagggga agtgactgag taaggattac
     5161 atacctcccg gttttgccgg gagttacagg acctgaaatt ctgcatttcg ttgctacatt
     5221 cattcaaacc acctgtaaca aaaagctgcc tttgacgagc tattgctata aataagagat
     5281 cttttttctt taattattta tttattttaa aatggttaag ttttgctaat catgatgagt
     5341 tatttttgat agttcatcct gtggcgtcgc tcttaaactt atattgtaaa tgacttagat
     5401 ttaaataaga taataatttt gttaacaata tgaggcggga gatgttttct acaaacttaa
     5461 tacagagtaa ttatggtgat ttaaatatta aaagcctggc tttcgactct tttaaagaaa
     5521 ggcttgagtc taccatggcg gcactcactt tctttatatc aacgggtcaa tgtgactgcg
     5581 atgaaatagc agagtcgaac ttcaatcgta tgattgccta tatgtctaac attaactatg
     5641 atgcctcaaa gctaggagcg ccagcactta gttttgatac gtatctgcag gac
//
LOCUS       DAJTQQ010000169         5635 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000169 DAJTQQ010000000
VERSION     DAJTQQ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.171"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(1..3727)
                     /gene="narZ"
                     /locus_tag="OO309_003910"
                     /pseudo
     CDS             complement(1..3727)
                     /gene="narZ"
                     /locus_tag="OO309_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            complement(3809..5197)
                     /gene="narU"
                     /locus_tag="OO309_003911"
     CDS             complement(3809..5197)
                     /gene="narU"
                     /locus_tag="OO309_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCR5848858.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
BASE COUNT         1382 a         1443 c         1487 g         1323 t
ORIGIN      
        1 cttgtacctg atcgcgacct tcatcatcca gccagttaac gttcttcatc ttgcggatca
       61 tgatgaattc gtcgcggttc gaaccgacgg tgccgtagta gttaaagccc caggccagct
      121 gcgcgtaacc gccaatcata tgcgtcggtt tcgggcaaac gcgggtgacc gagttatgaa
      181 tgccgccgcg catgccagtt acttccgaac caggaatatt cataatgcgt tcctgggcgt
      241 gatacatcat ggtcatgccc ggcggtacac gttggctgac caccgcgcgg gcagtcagcg
      301 cgccgttggc gttaaatact tccacccagt cgttatcgac aatggtcagt tctcgggcat
      361 ctgtttcgct gatccagaca atcggtccac cgcgagagag cgtcagcatt agcaggtttt
      421 cactgtaggt tgagtgaatg ccccatttct ggtgcggcgt caggaagtta agtgcttttt
      481 cctggaagcc gtttggcggt atctggcgca tctcactgac gctacgggtg tcgataggcg
      541 ggcgataagc caccaggctt tcaccaaaag cacgcatcca cggatgatcc tgatagagct
      601 gctggcgtcc cgacagcgtg cgccacggaa ttaactcatg aacgttggtg tatcccgcgt
      661 tataggatac gtgatcgctt tccagaccgg accatgtggg gctggagata attttacgcg
      721 gctgcgcctg aatatcgcga aagcgaatct tctcgtcctc tttgtgcagc gccagatggg
      781 tatgttcgcg cccggtgatc tcgcccagcg cctgccacgc tttgactgca acatgaccgt
      841 tggtttccgg tgccagtgcc agaatcactt ctgaagcgtc aatggcggtg tcaatcagcg
      901 gacgcccctg tgccgggcca tcacgcttgg tgtaattgag tttgccgagg aaatcgattt
      961 catcctgggt attccacgaa atccctttac cgccattgcc aagtttgtcc atcaatggcc
     1021 cgagcgaggt aaagcgttca tacgtagcag ggtagtcgcg ctccaccgcc acaatattcg
     1081 gcgcggtttt acccgggatc agatcgcatt cgcctttgcg ccagtcgagc acttcacacg
     1141 gctgtgagag ctctgccgga gagtcatgca gcagtggttg taataccacg tcggtttctt
     1201 tgccaagatg gcccacgcac acttgcgaaa atgctttggc aatacctttg tagatttccc
     1261 agtcgctgcg tgattcccag gccggatcga ccgccgcaga aagcggatga ataaatggat
     1321 gcatatccga ggtgttcata tcgtcttttt cataccaggt ggcggtgggc agaacgatat
     1381 cggagaacag gcaggtactg gacatgcgga agtcgagcgt caccagcagg tcgagcttgc
     1441 cttcaatcgc cgcagtttgc cactcgactt cttccggttt gatcccgtcg ctggcaccga
     1501 gttcctcgcc ctgaatcccg ctttcggtcc ccagcagata cttctgtata tactcgtggc
     1561 ctttgccgga ggagtcaagc aggttagagc gccagacaaa caaattacgc ggatgattgc
     1621 tgccgctatc tggttgttcg caggccatac gtaaatcgcc cgatttcagc gcctgggcgg
     1681 taaattctgt gggggataat ccggccttgt cggcttcagc tttaatcccg agcgggttac
     1741 gccccagctg cggcgcagaa ggtagccagc ccatacgttc ggcgcgaacg ttgaaatcaa
     1801 tcagatgacc gctgtactta ctggcatcgg cgagcggtga aagtaactcc tgagcagaga
     1861 ctttttcata gcgccattgg ctggaatgat tgtagaaaaa cgaggtgctg ttcatctggc
     1921 gcggtggtcg gttccagtcg agcgcaaagg ccagtggcaa ccagccggtt tgtgggcgca
     1981 gtttttcctg gccgacatag tgtgcccagc cgccaccgct ttgcccgaca caaccgcaga
     2041 agatcagcat attgatcatc ccgcggtagt tcatgtccat gtgataccag tggttaacac
     2101 ctgcgccgag gataatcatt gagcgcccat gcgttttatg ggcggtatcg gcaaattcgc
     2161 gggcgatggt ttcaatatac tggcgcggta cgccggtaat ttgctcaccc caggctgggg
     2221 tgtacggttt gatttcagcg taatctttcg cgctgttttc atcttccagc ccgcgatcga
     2281 ggccgtaatt cgccagtacc aaatcataaa cgctgaccac cggacaggtg ttgccatcga
     2341 ctaacgtcag gtttttaacg ggcaattggc gcaccagcac cggattgtgt tttacgctgc
     2401 gaaaatgcgg attttcaatg ccgccaaagt aggggaaggc cacgcctgca acagcgtcat
     2461 gttgaccgag cagggttaac gacaattcgg tttccgtacc ggcggcaatg gattccagat
     2521 tccatttgcc tttttctccc cagcggaaac caatcgaacc gttcggcact accaattcac
     2581 ctgcggtatt aactgctacg gttttccact gcggattgtt gctttcgccc agtccatcca
     2641 ccaggtcaga tgcgcggatc atgcgcccgg gaacgtagct accatcgtcg cgaggctcca
     2701 gcattaccag catcggcatg tcgctgtagc ggcggcagta gttgataaag tagtcgctgg
     2761 gattatcgag atgaaactct tttaaaatca catgccccat tgccatcgcc agggcgctat
     2821 cagtgccttg tttcggtgcc agccactggt cgcacaattt ggccacttca gagtagtcag
     2881 gggtaatggc gatggtttta gtgcctttgt agcgtacttc ggtaaagaag tgggcgtccg
     2941 gcgtacgtgt ctgcggtacg ttagaccccc aagcgatgat gtagctggag ttataccagt
     3001 cggcagattc cggtacgtcg gtttgctcgc cccaggtcat cggcgaggcg ggcggcaggt
     3061 cgcaatacca gtcgtagaaa ctttaacagg tgccgccaag cagcgacaga taacgcgttc
     3121 cggcggcgta agaaaccatc gacatcgccg ggatcggcga gaaaccggca acgcgatccg
     3181 ggccgtaggt tttgatggtc cagacgttag cggcggcaat cagctggttt agttcctgcc
     3241 agttggagcg gataaacccg ccgcgcccac gcacttgttt gtagctcagg cacttttgcg
     3301 gatcattcat aatcgatgcc cacgccagta ccggatcgct gtgttgtttg agggcttcgc
     3361 gccacagttc aatcagtcgt ttacgaatga gcgggtattt caggcggtta gcgctgtaaa
     3421 gataccagga gtaacttgcg ccacgcgggc agccgcgagg ttcatgattg ggcaggtcag
     3481 ggcgagtgcg cgggtagtcg gtctgttgga tttcccaggt caccagacca tttttaacgt
     3541 agattttcca gctacaggag cctgtacagt taacgccgtg ggtggaacgc acgattttgt
     3601 cgaactgcca acgctggcga tagctgtcct cccagtcgcg gttgctatgc atcacctgtc
     3661 cgtgaccatc ggcaaaggtt tcgccctttt gtttgaagta gcgaaagcga tccaaaagtt
     3721 tactcatgac atgactcctg ctccaggaat gaattgggat ctgatgcctt gtcggatgcg
     3781 acatggttgt acatcaggca tcgatctctt atttttggct gaacttccgc cgaccataaa
     3841 ccagccaggt cagcagcaca caaacgatgt agaagattaa aaacactttc atcgcgccga
     3901 ccggagagcc ggtcatattg agcgacatgc caaatgcctg cggaataaaa aagccgccca
     3961 ctgcgccaat ggctgagata aagcccagcg ttgccgccgt ttcggtgacg gcttctctct
     4021 gagcttgctc atcactaccg cctttcatct tcacccgata aatggttatc tggcgaaaga
     4081 tgacggcgat catctggaaa gtagaaccac ttcccagacc cgcggtcaga aacagcccca
     4141 taaatacggc gtaaaaggcg atgaaattac cggagcctgt gcccggtaag gtaaggaaca
     4201 gcagggcact gaaaatcgcc ataaaaataa agttgatcaa cgtcacccgc acgccgccga
     4261 atttatcgga aatagcacca ccaaccgacc gcgcgatggc accaataaat gggccaaaga
     4321 acgccaggcg cagaatattc acatccggga actgggtttt tgccagcatg gcaaaacccg
     4381 cagaaaaacc gataaacgaa ccgaaggtgg caaggtaaag caggctcagc agccagagat
     4441 gcaggcgttg taagacaggg agctggtcgg caattgaggc gcgtgaactg gcgatatcat
     4501 tcatccctga ccaggcggcg atcgtggcaa tcgccagtag cggtacccaa atccatgcgg
     4561 cattcgccag cgacatcacc gaaccgtcgg cctgcggtac gccattgacg ccgagaaagg
     4621 caaatacagg tacaaaaatg accagcggtg caaccagctg cattacactt acacctaagt
     4681 ttcctaatcc gccattaatc ccaagagcgc tcccttgttt ggcttttgga aagaagaaac
     4741 tgatattgcc catgctcgaa gcaaagtttg cacctgcaaa accgcatagc aaagcgataa
     4801 cgataaatat cccaaaagga gtattcggat tttgcacggc aattccgagc cagacgcaag
     4861 gaataatcag gattgcagta ctaaaaaccg gccatcggcg tccaccgaat ataggcacca
     4921 taaaggagta gggaacacgc aataatgcgc cagaaacgga gggtaatgcg gttaataaaa
     4981 agagttgatc ggtagtaaaa ttaaaaccga ttttattgag attaacggta actgcgctaa
     5041 atagcatcca gacacagaag gcaagaagta gacaactgac tgatatccag aggtttcttc
     5101 gagcaatatg ttttccttta ttttcccaga aggccggatt ttctggtttc cagtcgcgta
     5161 aaagataacg actatttttc tcattttgca gtgccatatt gttcctcata tgcacacatt
     5221 ggtaatgaaa aaagacaaaa caggaggtaa ggcgcaatag ccagttatta gaattaagga
     5281 tgaattgggt gaagtgctga ttgaaagaat agataagaaa gcgtaaccgc gggggcagaa
     5341 tggggattaa gtagccagat atgtgttact aaatgtaact aacaagctaa ccttcaaacg
     5401 ggggttaatc tttgaacgta tgtcatgttt caacccttca gatcgtgaat ctaaagggtt
     5461 acatattaac tatattctca acaagaccag acacttatag aattagtgat gatttattca
     5521 ctaaaaaggc catgaattgg aggaaaacga tctgtccaga aagtctatcg gttcctctct
     5581 tattgcctga ttgtaataat cgttattata gtaagtattg tttatttcat ctaag
//
LOCUS       DAJTQQ010000170         5614 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000170 DAJTQQ010000000
VERSION     DAJTQQ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.172"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            5..343
                     /gene="spa13"
                     /locus_tag="OO309_003912"
                     /gene_synonym="spaM"
     CDS             5..343
                     /gene="spa13"
                     /locus_tag="OO309_003912"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR5848859.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            330..1208
                     /gene="spa32"
                     /locus_tag="OO309_003913"
     CDS             330..1208
                     /gene="spa32"
                     /locus_tag="OO309_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR5848860.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            1202..2083
                     /gene="spa33"
                     /locus_tag="OO309_003914"
                     /gene_synonym="spaO"
     CDS             1202..2083
                     /gene="spa33"
                     /locus_tag="OO309_003914"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR5848861.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            2088..2738
                     /gene="spa24"
                     /locus_tag="OO309_003915"
                     /gene_synonym="spaP"
     CDS             2088..2738
                     /gene="spa24"
                     /locus_tag="OO309_003915"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR5848862.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            2753..3013
                     /gene="spaQ"
                     /locus_tag="OO309_003916"
     CDS             2753..3013
                     /gene="spaQ"
                     /locus_tag="OO309_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR5848863.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            3013..3783
                     /gene="spa29"
                     /locus_tag="OO309_003917"
                     /gene_synonym="spaR"
     CDS             3013..3783
                     /gene="spa29"
                     /locus_tag="OO309_003917"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR5848864.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            3790..4818
                     /gene="spa40"
                     /locus_tag="OO309_003918"
                     /gene_synonym="spaS"
     CDS             3790..4818
                     /gene="spa40"
                     /locus_tag="OO309_003918"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR5848865.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            4831..5073
                     /locus_tag="OO309_003919"
     CDS             4831..5073
                     /locus_tag="OO309_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848866.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT         1792 a          791 c         1032 g         1999 t
ORIGIN      
        1 agatgtggag gcattagata aaaggattat atacttcctg caactggaga atgacttaga
       61 acctgtaggc gctcagagtg ttagtcagct tttcaataca agacgtaaga tcgctatagt
      121 aaaaaagcat attatccaat atcagtctga acgaattctt ctaaagggaa gaattgaaga
      181 aattcaaaaa gatattgatg aggcaaatgc ttctaaaaga aaactgcttc ataaagagag
      241 taaaatatgt aaacggattg gcttgattaa aagaaataac tttgccaaac agttgatact
      301 tgatgagtta tcacaagagg atatgaagta tggcattaga taatataaac ctaaatttta
      361 gtagtgacaa acagatagaa aaatgtgaga aactatcttc aatagataat attgatagtc
      421 tcgttttgaa gaagaagagg aaggttgaaa ttcctgagta ctctttaatt gcatctaact
      481 attttactat tgataagcac tttgaacata agcatgataa aggagaaatt tatagtggca
      541 ttaaaaatgc gttcgaactt agaaacgaac gagcgacata ttctgatatt ccagaatcaa
      601 tggccattaa agaaaatatt ttgataccag atcaagatat caaagcaagg gaaaaaataa
      661 atatcggcga tatgaggggg atcttttcat ataataagag tggaaatgca gacaagaact
      721 tcgaaagaag tcatacttct tctgtaaacc ctgataatct gctagaatct gataatagaa
      781 atggtcaaat tggtttaaaa aatcatagct tgtctattga taagaatatt gctgacatca
      841 tttctttact aaatggaagt gttgctaaat catttgagct gcctgtaatg aataaaaata
      901 ctgcagacat aaccccatcc atgtcattgc aagaaaaatc aatagttgaa aatgataaaa
      961 atgtttttca aaaaaatagt gaaatgactt accactttaa acagtggggg gctggacatt
     1021 ctgttagtat ttcagtggag tctggttctt ttgttctaaa accgtcagat caatttgtag
     1081 gaaataaact tgacttaatt ttgaaacaag atgctgaggg taattacaga tttgatagca
     1141 gtcaacataa taaggggaat aaaaataata gtacaggata taatgaacag agtgaagaag
     1201 aatgctaaga attaaacatt ttgacgctaa cgaaaaacta cagattttat atgcaaagca
     1261 actctgcgag cgtttttcga ttcagacatt caaaaataaa tttacaggca gtgaaagttt
     1321 agtcactctt acttccgtgt gtggggattg ggtaattcgt attgatacat tatctttttt
     1381 gaaaaaaaaa tacgaggtat tttcaggatt ttctacacaa gaatctttac tgcatttatc
     1441 aaaatgtgtc tttatagagt cgtcatctgt attttcgatt ccagaactgt ctgataagat
     1501 tactttccgg atcacgaatg aaatccagta tgcaactact gggagtcatt tatgctgttt
     1561 ttcctcttct ttaggtatta tttattttga caagatgccg gtattacgta atcaagtttc
     1621 tcttgactca ttgcatcatc ttttagagtt ttgcttaggt tcatctaatg taaggctggc
     1681 tactttaaaa agaattcgca ctggtgatat aatcatagtt cagaaacttt ataatttatt
     1741 attgtgtaat caagttatta ttggggatta tattgtgaat gataataatg aggcaaaaat
     1801 taatctgtca gaaagtaatg gtgagtcaga acacacagaa gtttctttgg cattattcaa
     1861 ttatgatgat atcaatgtaa aagtggactt tattctttta gaaaaaaata tgacaatcaa
     1921 tgaactaaaa atgtatgtag aaaacgaatt attcaagttt cccgatgaca tagttaaaca
     1981 tgtaaatatt aaagtaaatg gttctttggt tgggcatggg gaacttgttt ctattgagga
     2041 tggttatggt atcgagatta gttcttggat ggtaaaggag taatctcatg ctgagtgaca
     2101 tgtccctcat cgctacactt tcttttttta cgctattacc atttttagtt gctgctggta
     2161 cctgttatat aaagttttct attgtttttg taatggtacg aaatgcactt ggtttgcagc
     2221 aggttccatc aaatatgact ctcaatggta ttgcattgat tatggctttg ttcgtgatga
     2281 aacccattat tgaagctggt tatgaaaatt atctaaatgg ccctcaaaaa tttgatacaa
     2341 tatctgatat tgtccgtttt tctgattctg gcttgatgga atataagcaa tacttgaaaa
     2401 agcatacaga tcttgagtta gcacgttttt tccaaaggtc tgaagaagaa aatgctgatt
     2461 taaagagtgc agagaataat gattattcat tgttttccct cttgcctgca tatgcgctaa
     2521 gtgaaatcaa agatgcgttc aaaataggtt tttatttata tttgccattt gttgttgttg
     2581 atcttgttat ttcaagcatt ttacttgcac ttggtatgat gatgatgagt ccaatcacaa
     2641 tatcagtacc aataaaactt gtattatttg ttgcgcttga tgggtggggt atcctgtcta
     2701 aggccttgat tgaacaatat atcaatattc ctgcttagat gatggaggta atgtgtctga
     2761 tatagtttat atgggtaata aggctcttta tttaatcctt atcttttcct tatggccagt
     2821 aggtatagct acggttattg gattatctat tggtttgtta cagacagtga ctcaactcca
     2881 agagcagaca cttccttttg gtataaagct tataggtgtc tcaatatctt tgctacttct
     2941 ttctggatgg tatggtgagg ttttattgtc tttttgtcat gaaataatgt ttttaattaa
     3001 gagtggggtt tgatggacat ttcaagctgg ttcgaaagta ttcatgtgtt tttaatactc
     3061 ctgaacggcg ttttttttag attggctcca ttatttttct ttcttccatt tttaaataac
     3121 ggtataattt ctccatctat tagaatacct gtgatttttc ttgttgcatc aggattaatt
     3181 acttctggta aggtagacat aggttcttct gtttttgaac atgtttattt ccttatgttc
     3241 aaggaaataa ttgttggcct ccttctctct ttttgcttgt ctcttccctt ttggatattt
     3301 catgctgttg gtagcattat tgacaaccag cgtggggcaa cgcttagtag ttcaattgat
     3361 cctgccaatg gtgttgatac gtctgagttg gcaaaatttt tcaatctttt ttctgcagtt
     3421 gtatttctat acagtggtgg tatggtcttt attttagaat ccatacaatt gtcttataat
     3481 atatgcccgt tattttctca atgttctttc cgtgtctcaa atatcttaac atttctgact
     3541 ttattggcaa gtcaggctgt tattttagcc agtcctgtta tgatagtatt gttactatca
     3601 gaagtattac ttggtgtatt atcgagattt gctccgcaga tgaatgcttt ttccgtatca
     3661 ttaactatta aaagtttact tgcaatattt attattttca tctgttcttc tactatttac
     3721 ttttctaaag ttcaattttt cctcggtgaa cataaatttt tcacaaatct atttgttaga
     3781 taaaatatta tggcaaataa aacagaaaag ccgacaccta aaaaactaaa ggatgccgca
     3841 aaaaaaggac agtcatttaa atttaaggat ttaacgactg ttgttattat tctggtaggg
     3901 acatttacta taatatcatt cttttcctta agtgatgtaa tgctcttata cagatatgta
     3961 ataattaatg acttcgaaat taatgagggt aaatactttt ttgcagtggt tattgtcttt
     4021 tttaagataa ttggcttccc actttttttc tgtgttcttt cggctgtgtt gccaacattg
     4081 gttcaaacaa agtttgttct tgcgactaaa gctatcaaga ttgatttttc agtattaaac
     4141 cctgttaaag ggttaaaaaa aatatttagt ataaagacaa taaaagaatt tttcaaaagc
     4201 attctgcttc ttattattct agcattaaca acctatttct tttggattaa tgaccgaaaa
     4261 ataatttttt ctcaggtgtt ttctagtgtt gatggcttat atcttatttg ggggaggctg
     4321 tttaaggata taatattatt tttcttggca ttttctattc ttgttattat tcttgacttt
     4381 gtgattgagt tcattttata catgaaagat atgatgatgg ataaacagga gataaaaaga
     4441 gaatatatag agcaagaggg acactttgag acaaagtcga gaaggcgtga gttgcatatc
     4501 gagattcttt cagagcagac taaatctgat atacgtaatt caaaattagt ggtaatgaac
     4561 ccgactcata ttgcaattgg tatttatttt aatccagaaa tagcgcctgc accttttatt
     4621 tctctcattg aaactaacca gtgtgccttg gctgtcagaa aatatgcaaa tgaagttggt
     4681 ataccgactg tgcgtgatgt gaaattagct agaaaactat ataaaacaca tacaaaatat
     4741 agttttgttg attttgaaca cttggatgaa gtcctacgtc ttattgtttg gcttgagcag
     4801 gttgaaaaca ctcattaata agtaaaggag atgtgttata tgggagttaa tttctgtaat
     4861 aaaataggta ttgatcagtc tgaatttgaa atagaatctt ccatcattaa ctccattgct
     4921 aatgaggtat tgaacccaat atctttcctt agcaataagg atataataaa tgttttactc
     4981 agaaagattt catctgaatg tgacctggtt agaaaagaca tttatcgttg tgctctggag
     5041 ttggtcgttg aaaaaacacc tgatgattta taatcattca taataactct tttgctgata
     5101 taaatatttt tgttgaagat atttatgtag tcacaggatc aattgtagaa aattgttatg
     5161 tttttgttag gaactgcact aaataatata tgataagaca gcaaaaacga ctgaccataa
     5221 tccttctttt attaggagtt gataagagag actattcatc ctgtaatgtt aaaactctgt
     5281 tatatagtat tcgcgattat gcaaagtctg taaatgaaca tgagatacta accgagagta
     5341 atcgtttatt atctcattgc atttcagata gtaacggagc tttctttaaa agtagtaaat
     5401 acgttcccct taagtactta cgtaaaagga gaatagcacg aaagatacca aatgattagc
     5461 actttgctag taagcttgct ggtaaactat attgaccgtt gggtttgttt tgagactgta
     5521 atttaaattg tgtaattgcc tgtttttgat atgtttactc caacaacgga gacaggtaaa
     5581 ttatggacga aaagaagctt aaagcccttg cggc
//
LOCUS       DAJTQQ010000171         5601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000171 DAJTQQ010000000
VERSION     DAJTQQ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.173"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..411
                     /locus_tag="OO309_003920"
     CDS             <1..411
                     /locus_tag="OO309_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5848867.1"
                     /translation="AVRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDA
                     LCYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGI
                     DPEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            443..1057
                     /gene="istB"
                     /locus_tag="OO309_003921"
                     /pseudo
     CDS             443..1057
                     /gene="istB"
                     /locus_tag="OO309_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="OO309_003922"
     CDS             complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="OO309_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HCR5848868.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1378 a         1402 c         1333 g         1488 t
ORIGIN      
        1 gccgtcaggc agcagcgaag caaagcgtta ctgacgtcgc tgaatgaatg gatggtggag
       61 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
      121 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca
      181 gcagaaagag cgcttcgtac cgtctgtctc gggaaaaaga attacatgtt cttcggcagc
      241 gatcatggag gcgatcgcgg agctctgctg tacggactaa tcgggagctg tcggttgaac
      301 ggtatcgatc cagaagcgta tctgcgccat atcctgagcg tactgccgga atggccttcc
      361 aaccgagttg acgaactgct gccatggaac gtagttctca ccgataaata agcgtcaata
      421 cggccttccg ttgacgctta cagaactcct gtggcgcgag agtgaaaaac tgcgtcgtta
      481 taaaaaggag gcccggttgc cagttgccaa aacgctaagc gaatacgact tcattcagtt
      541 gccggaactg aatggagctc agttccagca actctgtgaa acgacagact gggttgatgc
      601 tggagaaaac gttctgctgt tcggggccag cgggttgggg aaaagccatc tggcggcagc
      661 gatcgtggat ggcgtagtag gccagggcta ccgggcccgg ttctacagcg caggagagct
      721 gttgcaggaa ctacgtaaag ccagagctca gttgaaactg aatgagctgc tactgaaact
      781 ggatcgctac cgggtgatag tggtggatga tcttggctat gtcaaacgcg acagcgccga
      841 aacgggtgtg ctgttcgagt taatagcgca tcgctatgaa cgtgggagcc tggtgataac
      901 cagtaaccat ccgttcagca tgtggggcag catcttcgtg gatgagacta tggcggtggc
      961 ggcagcagac cgactgatcc atcacggata tatgttcgaa ctgaaagggg aaagctacag
     1021 gaaaaagaca gcgaaggcag taacaagcgt gacttgatgt cgcactgaag gggcggccag
     1081 tatagttggc gccagtcggc aaaactagtt gacgtctaat acctggacgg tcacttctga
     1141 gaaaaggcat ttacacagaa tcttaaacag gctcacggag ttctttcaga aaaaggcctg
     1201 ccgaatggca gacctatccc attcgtcaga aaacataacg gaagttggcg tttatcgcat
     1261 tatccacatt atatttcccg aacgctgatt tctccagctc cagtcccaaa cgcatattat
     1321 ccttaatttc cgcattcatc cctaccgtca tcagcatcct gctatctttt tcaccttcga
     1381 aacgtttctc tccggaagca tcctgcagaa ccgtttctcc gttcgccagc aggtcgaact
     1441 gataacccag cccggctcga gctgtgattt tccagtcgtc tccggagaag gctcttccca
     1501 cgtcaacacc agtacggcca atcagtgggt tataatcctt gtctttcatg ctcagagcca
     1561 ttccccggtc ttcccagcta aaagcttttc ctgatacaga accgtaaacc agctctatct
     1621 gtggctccac ccaggactct ttcgtcaggt ggtaacgata accaacttca gcaccggcat
     1681 accaggaatg agagctgtag tcttttgttc ccagtgaggc aaaattcgcc gtgtgctgat
     1741 tatcatgatg gagatattta ccaatcaggt caaaataagc tccggaatta aagagtgcag
     1801 aggcatacag accgccaccc acggatttgt tttttccact gaatgcgtgg ctgcttgcat
     1861 tgctgtccgt ataggtcagc aatgcccccg taaataaatc cacaccgtcc agctcatgct
     1921 ttctgtcgac accaatctga acgtgagtgt agttatcgct gtagtcacca tctgccgaac
     1981 cggtaccatt cattatgcgt gcccagacac cggcatcccc ctgagtatcc cgcaggtcac
     2041 ccatacgttt gttcaggtta ttgacctctg taaggaagga cttataacct gatgccataa
     2101 agtcttttgc ggctttcgag gctccggcat cagcggtagt ctgatatcct gtcagcaccc
     2161 attttgtggc atcatccgtt ttttccgtgc tgattactgg cgtaacgtta ctgaaaccaa
     2221 tctgctgcgt accggcagtg aacgttttct catcactccc tttcggtgcg gtaattaacg
     2281 tcacgctcat ttcactggct ggtttgttta aaaagtcaac caggacgcta ttattactgc
     2341 ctgacagttt gtctgtgaca ttcaactggt cagcccggct gttgttggta tgcatcagga
     2401 acgtggtatt atctgcagaa agctctttta cttgtaaagt ggcaaagtgt ttcccgtccc
     2461 ccagttcaat tcgcgagccc ttagccttca gtgcaccggc ctctgatttt ccgtcagagt
     2521 accacagagc attgatcata tttaccgtac tctgtgcccc cgtaatactg cctctcagaa
     2581 cagcgttata tcccttcatt aaggagctga ataacggaga gtccgtcttc cctgcatcag
     2641 catcactgtc tcccagatta atggttgccg cagtggttcc tgcatgaata tccgaactca
     2701 gatagcccat atttttaaca gaaaagctgg cgttatcttc gaggttaatc ccttctgttg
     2761 tagaaattac gggtgagtaa tacccctgtt tctgcgcaga aggcatcccc gtcagggtca
     2821 gagaagcatt ttcttttaca tccacagccc cggtgctgaa aataccgctc tgagccgtaa
     2881 gttttgctcc ttttccgagg gtcaggctgc tgttgacaaa gctgccaaca cggtcaaaaa
     2941 cagcattctg agaggtcaca ctcacggtgc tatcataagc ctcaatacca ccggtgaatc
     3001 tctccatgat ttgcagggat gatttatttt ccagcgtaac atttccgctg taatcactct
     3061 ggtcaccaac tgtggttgca gtggcactcc cttcaacaga ggagaccgca ttaccggttc
     3121 cgtcactcct gtcagtaaag acacggctac ttcccagaat gactgtactt ccattggtgg
     3181 cgttaatatc tccctgcatc ttgccgtttc tggacagatg gaatgttgcc gcatcgagtt
     3241 tcagtttttc cagcgtaaac tgacgggttt cccagtcgct ctggctggaa gaagtcgtcg
     3301 ttccagcatg aataactgga tgaccctgga atatcagcgt gccggacttc ttggttatcg
     3361 aaccttctgg caggttaact gaaccatcaa gagccagtac atccttacct gagagctgcg
     3421 gaatattcac atccatattg ccgtttagtt ggccgtgata catataaggc tggctgctgg
     3481 cctcaatttt ttgttgcttc acagtatcaa tggctttatt acgatctttt ccgacattct
     3541 gccagacact gctgttgttc agatcggaga agaacgggga atatgaactc gctttcagga
     3601 tgaaatattg cttggtagaa ctgtcataat ataggtctcc cggcgctcct ctccccccaa
     3661 aaatactgac agtattaacc ggtacattga tgtcactggc ttttaatgtc tgaaggtcga
     3721 gtgttaccgt tgattttttc tcactgttat tggtaatgat ggccccgtag tcagccgcct
     3781 gaagacgggt aaaggtcaga ttattaccat tcagttccag acgtcctcca cggtatcccc
     3841 acgaaatatt atccggatta acttgctgtg agtcagaaag cacaactgtc ggacgaccgc
     3901 tggcaatacc aacagagctg aaagcctgta cctttccgtc agcatccgcc tgctggttga
     3961 gaataacggt accgtccccc accttgagtc cgcctgcatt cacaccggtc ccgtttactg
     4021 tcagggtacc ttcgccagtt ttatgcaggt tatcaccggc aactccgttc acctgccaca
     4081 gaacgtgggt tcctttatcg gtgataatcc cgccccccat ccatgtctgg ccgttcaggg
     4141 cggacaccct gtaatcgccg gcaaactgca gatacccagc tccctgatta acggaattct
     4201 gcagaacgac ctcgccatta ttgccggtga acaggaggtt ttttcctgcg ttcagatcat
     4261 ttcctttctt tccatgcata tcccaagtcg tgtttccctg agtcagtgtg ccggtgccat
     4321 tagccgcatc atatttccac tgcagtactc cttcaccaga ggtgtaggct atagttttgt
     4381 caaaatcatt ttgcggttgc tgaccaagga aatcctgagt ggtgacgacc cagttatttc
     4441 catagttagt tcctgaggaa agcacgccaa tcagtaccca ttttttttgc agggaatcat
     4501 aagcaaacag gggggagccg ctgtccccca tattcagata attagccaga ggtccattcg
     4561 cgggattaaa aatgtcaccg gtctgagctg taatcatctg tccgttgtta taggagttca
     4621 gcgcgctgac ggttccccca acaagaaatc cgccattggt atacacttgg gtgcgattac
     4681 catttttgtc tttgacgtac tgcatccccc ccccaaggcg atagaattca gtaaagcgtc
     4741 cgccggcctg ataggcaccg ctcacagccc cgatgtcaga cacttctgcc ggcgccacct
     4801 ctgtcaccag tttactcagg cggcgggttt taatatcaag accgctatta ttatttgtac
     4861 caaccgcagt ataggtgttt ttggcgtatc cgaagctcat cgtgtcagaa ccgctgacat
     4921 gtttggcggt aaccacatac tgaggggagt aaagtgtatg gtctcccgga ggcaggctac
     4981 cggtagtaat agtggcacta ctgaaatcag ccatcggggc tttatcaagt ttaccgacca
     5041 gattgccctg cttgtcgtaa atggaaatat tcgtggtccc cggcgtaaac tgacctttat
     5101 tctcggcaaa gtcgcggaat atctgataag ggatctctgc actgaccgtt gctgacagag
     5161 ctggccatgc cgaggaaagt gataaagctg caacagacgt aagaataacc cgacgtgaaa
     5221 gtctgcgatg gcttttgcag gtaaccctgc gggccagttc ggagacagca atcaggcttt
     5281 tggttatatg gcaatactta agataatata ttttgttcat atagaaacat caacctaatt
     5341 ttgacaaaga ttaattacat aggatgtaga tggagcagtc cgagattcta ctaatatcaa
     5401 ttattaagca tctgtttttt agttggtgta atataatttt tattaaggta agcggaaaaa
     5461 caaccaaaaa aaacgcaaga tgttattttt ttatcatttt gagccagttt ggctgggggg
     5521 atactcttct cttaagcttc ttgctcaact ttaaaaatag ttatgccagt tcggtatcga
     5581 taaggggcag tttggatatc g
//
LOCUS       DAJTQQ010000172         5596 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000172 DAJTQQ010000000
VERSION     DAJTQQ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.174"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..372
                     /gene="mddA"
                     /locus_tag="OO309_003923"
     CDS             <1..372
                     /gene="mddA"
                     /locus_tag="OO309_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR5848869.1"
                     /translation="GYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQG
                     KGLGRKLLSRLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGH
                     WLDLTFMQLQLDERTEPDAIG"
     gene            369..818
                     /locus_tag="OO309_003924"
     CDS             369..818
                     /locus_tag="OO309_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5848870.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(819..1052)
                     /locus_tag="OO309_003925"
     CDS             complement(819..1052)
                     /locus_tag="OO309_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR5848871.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1138..1311)
                     /gene="ortT"
                     /locus_tag="OO309_003926"
     CDS             complement(1138..1311)
                     /gene="ortT"
                     /locus_tag="OO309_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR5848872.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1506..1601
                     /gene="yncL"
                     /locus_tag="OO309_003927"
     CDS             1506..1601
                     /gene="yncL"
                     /locus_tag="OO309_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR5848873.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2003..2812)
                     /locus_tag="OO309_003928"
     CDS             complement(2003..2812)
                     /locus_tag="OO309_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCR5848874.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3022..4446)
                     /gene="patD"
                     /locus_tag="OO309_003929"
     CDS             complement(3022..4446)
                     /gene="patD"
                     /locus_tag="OO309_003929"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCR5848875.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4468..5262)
                     /locus_tag="OO309_003930"
     CDS             complement(4468..5262)
                     /locus_tag="OO309_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5848876.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5252..>5596)
                     /locus_tag="OO309_003931"
     CDS             complement(5252..>5596)
                     /locus_tag="OO309_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5848877.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT         1472 a         1425 c         1236 g         1463 t
ORIGIN      
        1 ggttatccgg tgctggtaag tgaagaagat ggcgtagtta cgggatatgc ctcgtttggc
       61 gactggcgta gtttcgatgg ttttcgccat accgtggaac attcggttta tgtccatccc
      121 gatcatcagg gcaaaggtct ggggcgtaaa ttgttaagtc gattgattga tgaagcgcgg
      181 gattgcggga agcatgtcat ggtcgccggg atcgaatcgc aaaatcaggc ttcgctgcat
      241 ctgcaccagt cgctgggatt tgtcgtcacc gcacaaatgc cgcaggtagg cactaaattt
      301 ggtcattggc tggatctgac atttatgcag ttgcaactcg acgagcgcac tgaaccggac
      361 gcgattggat gaatcagtcg ctcacccttg cttttttgat tgccgccgga attggtctgg
      421 tagtccagaa cacgctgatg gcgcgcatca cccagacctc ctctaccatt ctcatcgcca
      481 tgttgctgaa ctcactggtg gggattgtgc tctttgtttc tattttatgg tttaaacaag
      541 gcatggcggg gtttggcgaa ctggtgtcca gcgtgcgctg gtggacactt atacctggct
      601 tgctgggttc gttttttgtc ttcgccagta tcagcggata tcagaatgtg ggagcggcga
      661 caactattgc ggtactggtc gccagccagc tgattggcgg tctggtgctg gatatcttcc
      721 gcagtcatgg cgtaccacta cgggcgttgt tcggcccaat atgtggcgca attttacttg
      781 tggttggcgc ctggttggtg gccagacgct cattttaatc agaggatggt gccgcctttg
      841 gttaactgtt cgtggcgcgc ttccatatct tctttatgct tgcgaccgtg atgagcaata
      901 gcggtgcgca aacgctgctg ctgggtgtag cgatcttccc gacttaactc cgcgtcatcg
      961 ctcagggcga ttaataattc gttcatatgg gcaatgacgc tctcttcaat cgcggcatcc
     1021 acgcgcgcga tgacttcatc cagatgcgac atactttctc cttagccact tgtgaaattt
     1081 atcgattaat ccaaatgata taagtctgcg tggtgccgtc aagccagtga gtaagattta
     1141 aaacagtgaa aacaacagcg ccaccgggaa actcatcggc catgtggccc ccaccagaaa
     1201 ggcgcttaag aaacggatgc gtttcttatc gtgagaaaga aaccaggtga taagaaatgc
     1261 gattgctgcc ataaccgcat aaaaaacaag catatgttga tagagaaaca tgtacgggcg
     1321 tccagccata tgaataaaac gcgcgcaaat atgcgccatt ttttgacgtg catcaagttt
     1381 tttcaccagt ttgagaaaaa atcatcaaac agcaacttgc ccttatccat cttcagaatg
     1441 agtattattg cggattttct taacccgtac tatacacata ggggcaatga taaaaggtgg
     1501 caaaaatgaa tgtttccagt agaactgtag tactgataaa tgtctttgct gctgttggtt
     1561 tgtttactct tatctctatg agatttggct ggtttatttg atgtagtgat tttgcacgca
     1621 gcacgtactg tcagagaaat caactctctg acagtacgca agatcttctc atactctcca
     1681 agtttgcaac attatcctta atatttactt ctttttgctg gcatctacat tgaaatgagt
     1741 agaaaacatc cccctactgt cgtctataca aacagcttac tgattcaaac ggaatcttgt
     1801 tttcacattt ttctatcacg aatactttat acgcgcggga gtctataaat gctttatact
     1861 ttcaaggaat aacgtaaata taaaaagatg gcgtaaagta tatgcatggc aggatgtttg
     1921 caacctcctt gatgacgctg ccccattgaa attgcaaacc ccgccctggt tatcttgccc
     1981 atttatcatg caaaacagat aattataggc ctctgaatac ctcagaaaga ttgaattttt
     2041 tatagtccct ctcttgaata tttccatttg atatcaatcg attcatctct tcaacaaaat
     2101 aatttatatc atcacactta ttattatccg gaagatctga gtagttataa ttccaccatt
     2161 ttattctcaa cagttcatca ataacatttg aatcaaacct aaatctgatt atttttgcag
     2221 gaactcctgc aactatggca tatgggggca catctttggt aacaacggaa ttggcagcaa
     2281 taacagcacc atcacctatt gcgatgccac ctttcaatac aacatcattt cctatccaga
     2341 catcatttcc tataatcgga ttttcaggtt tggtaatgaa tggtttcaat gtccagctac
     2401 gttcaaattc actcatagca aacttatcaa attcaccgtt ataagtcatc atgtgagtag
     2461 taaaacgatt caaaggatga tttccaccca ttatttttac atttgatgca atactacaaa
     2521 atcgacctat acgtatattc cctgaaaaat ggcaggtcgg aaatgaaaaa gcgccgacac
     2581 tggagaaatt tttcacaggc attgttgaat attcttcagc aataacatta tcttcaattg
     2641 ttatcttgtc accttttgag aaaagaaata aattcctttc tttatacgac ttcttcccct
     2701 tatgatttaa aaagatttta ttatccatac aaaactcaaa gtgttttttc gtccatctga
     2761 aggtaagggt tttattttta tcttccgtga atttgtacaa ttgaactttc attgttaata
     2821 cttctactta taaagtatta gtttaataac gatactctaa agccttatgc taatcaactg
     2881 tgacaggatg aaaaatcgcc atcaaacatt tagcctaatt tttacgccag ttattaatgt
     2941 aatcattcta atacttaacc gccattaaac tcagaaccta aaacgatagt ggccgtccta
     3001 taacagccac taacatctaa cttaatgttt aaccatgacg tggcggacga cggtgtaatc
     3061 ctccagccca taaagtgaca tatccttgcc gtaaccagaa agtttctgcc caccgtgcgg
     3121 catttcactt accagcatga aatgggtatt gacccaggta caaccatatt gcagccgtgc
     3181 gctgacgcga tgcgccctgc ccacatcttt cgtccatacc gaagatgcaa gtccgtactg
     3241 gctgtcattc gcccagttca ccacctgttc ttcgttgtcg aagggcgtaa cactcactac
     3301 tggaccaaat acctcttttt gcacgatggc atcgtcctgt aatgcgccag ccagcagcgt
     3361 cggcgcatag taatagccat tacccttgcg cttttcaccg ccagtgatca ctttgatgtg
     3421 ccctgtcgct ttcgcctctt ctactgcctt gctgacgcgt tcgagatgcg ccagcgagct
     3481 taaaggtcca agctccgtag actcgtcatc cggcgcacca gattttaacg ttgccaccgc
     3541 agcacccagt ttttccacca gcgtatcgta aatgcctttt tgcgcgtaga tccgacaagc
     3601 cgcagtacaa tcctgtccag cattgtaata gccaaatgta cgtacacctt cgaccactgc
     3661 ttcaatatcc gcatcatcaa aaacaatcac tggcgctttg ccaccaagtt ccatatgagt
     3721 acgcttaatg gacgacgcgg tatggctgat gatgtgctcg ccggtggcga tagagcccgt
     3781 cagcgacacc atccgcactt tgggatgacc ggtcagcgga tcacccaccg ttttgcctct
     3841 gccaaacagt atgttaatca cgcctgccgg gaagatatct ttcgccagct ctgccaactt
     3901 caacgcggtc agcggggtaa tttctgatgg tttaagcact acgcagttcc ctgccgccag
     3961 cgccggagca agtttccacg cggccatcat cagcggataa ttccacggtg cgatagaagc
     4021 cacgaccccc aacggatcgc gacggatcat cgaagtatga ccttcaagat attcacctgc
     4081 cgccagacca ttcagacagc gcgccgcacc cgcgaaaaag cgaaaaacat cgacaatcgc
     4141 cgggatttca tcattgaacg cactatgcag cggtttgcca caattacggg attccagttc
     4201 ggcaaaaacc tgaccatttt cttcgataac atccgccagt ttcagcaggc attccgcacg
     4261 cgctttcggt gtggtttgcc cccattcagc aaatgctgca tctgccgcac gcacagcagc
     4321 atcgacctgc tctgcggata cctcggcaat ctccagtaaa acgtcccccg ttgccggatt
     4381 atagacaggc tgtttttccc cttcgccgct aacccgttct ccgttaatca gtaacttatg
     4441 ttgcatagct tattcctgta tcagtggtta ttgaccattg tcgccttcgc gggttagcca
     4501 ccaggccccc aggatcggca aggttgttac caacataacc agcagtgcca ccacgttggt
     4561 taccggtaca tcacgcggtc gcccaagctg attgagcaac cacaacggta acgttcgttc
     4621 atgacctgcc gtaaaggtcg taacgatgat ttcatcgaac gacaaggcaa acgccagcat
     4681 tcctcctgcc agtaacgccg aactgagatt cggcaacact acgtagcgga aggtttgcca
     4741 gccattggcc ccaagatcca ttgacgcctc aaccagactc cacgaggtgc ggcgaaaacg
     4801 ggcgatgaca ttgttaaaca ccaccactac gcaaaaagtc gcatgaccga ccacgatggt
     4861 gaaaaatccc ggctccagat tgatggtttt aaaggcggtt aatagcgcca gaccagtgac
     4921 aatgcccggc agcgcaatgg gcagcagcaa taacagcgaa atggcgtttt tgccgaaaaa
     4981 gtctcgtctc cacagcgcgg cagctgccag cgtccctaac accagcgcaa ttaatgtcgc
     5041 cagcgccgcc actttaagtg acagcgtcac ggcatcaaga atatcactac gctgtgctgc
     5101 cacgctaaac cagcgcagcg tcaggccctg cggtggaaaa ctaaacgccg catcttcagt
     5161 gttaaacgca taggcggcga ttatcaggat gggaaaatgt aggaaaacaa cgccgcccca
     5221 ggccgccagt ttgaggaaaa acggtgcgcg ttcagagtgc atcgaacgct cccagacgtt
     5281 tcacgaacgc caggtacagt gcgatgagaa taatcggcac cagggtgaat gccgccgcca
     5341 tcggcatatt gccaatcgcc ccctgctggg aataaaccat attgccgata aaatatcctg
     5401 gaggaccaac cagctgcggg acgataaaat cgcccagtgt gagtgagaag gtaaagatag
     5461 agccagcggc aatacccggg attgccagcg gcagcaccac atagcgaaag gtttgtcgtg
     5521 gacgtgcgcc gagatcagcc gacgcctgca acaatgacgg cggcaaacgc tcaagcgccg
     5581 cctgaacggg caggat
//
LOCUS       DAJTQQ010000173         5590 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000173 DAJTQQ010000000
VERSION     DAJTQQ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.175"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(1..279)
                     /locus_tag="OO309_003932"
     CDS             complement(1..279)
                     /locus_tag="OO309_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848878.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            220..405
                     /locus_tag="OO309_003933"
     CDS             220..405
                     /locus_tag="OO309_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848879.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="OO309_003934"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="OO309_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5848880.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="OO309_003935"
     CDS             complement(1254..1670)
                     /locus_tag="OO309_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848881.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="OO309_003936"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="OO309_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2862
                     /locus_tag="OO309_003937"
     CDS             1837..2862
                     /locus_tag="OO309_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5848882.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(2915..3004)
                     /locus_tag="OO309_003938"
     tRNA            complement(2915..3004)
                     /locus_tag="OO309_003938"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2968..2970),aa:Ser,seq:cga)
     gene            3098..3895
                     /gene="mtfA"
                     /locus_tag="OO309_003939"
     CDS             3098..3895
                     /gene="mtfA"
                     /locus_tag="OO309_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR5848883.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3996..4071
                     /locus_tag="OO309_003940"
     tRNA            3996..4071
                     /locus_tag="OO309_003940"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4029..4031,aa:Asn,seq:gtt)
     gene            4233..5495
                     /locus_tag="OO309_003941"
                     /pseudo
     CDS             4233..5495
                     /locus_tag="OO309_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1508 a         1326 c         1293 g         1463 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt aattccaagt gtgatttttg
     2881 tcactgactt aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc
     2941 ccggtggagt tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct
     3001 ctccgttcag atggttgcca tgatgccagg aaatttggca ttttaacagt ccctgtccgt
     3061 gcaattttgt tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt
     3121 acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt
     3181 aacgtgtctg acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca
     3241 gcaaaaacgg cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat
     3301 agcacttcta ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca
     3361 tgaagtctta atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg
     3421 tctggtgcat aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt
     3481 tttgaactgg ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca
     3541 tgaagtcgct cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat
     3601 ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca
     3661 ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ctgccagtga
     3721 tcctgctgaa tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc
     3781 tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca
     3841 gagactgcat cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac
     3901 aactttgaag attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag
     3961 gtaccgctaa gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac
     4021 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt
     4081 tttcatgcat ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc
     4141 ttctcggtgc attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga
     4201 tcaggttagt tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca
     4261 ccctcaagcc ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg
     4321 tcaagccggg tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc
     4381 gcattgcgct gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag
     4441 gtatccgtaa aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg
     4501 gctcacgaac accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca
     4561 ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc
     4621 cggtcatcgg gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga
     4681 aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta
     4741 acataatgcg ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg
     4801 gcggcgtgac cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc
     4861 ctgaactgct tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg
     4921 ttctgctgat gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag
     4981 agattgattt cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg
     5041 tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac
     5101 agtccatcgc cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc
     5161 ccggcgacca taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg
     5221 tgatgggtta cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca
     5281 gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc
     5341 aggagcgcaa taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca
     5401 aagcgatgat gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc
     5461 cttatacaat tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg
     5521 caaattacca ccacctctac aggaggtggt tctatataga cttttatcaa cttcatgacg
     5581 aaaaacacct
//
LOCUS       DAJTQQ010000174         5418 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000174 DAJTQQ010000000
VERSION     DAJTQQ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.176"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            368..1294
                     /gene="tauA"
                     /locus_tag="OO309_003942"
     CDS             368..1294
                     /gene="tauA"
                     /locus_tag="OO309_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5848884.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1307..2074
                     /gene="tauB"
                     /locus_tag="OO309_003943"
     CDS             1307..2074
                     /gene="tauB"
                     /locus_tag="OO309_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR5848885.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            2071..2898
                     /gene="tauC"
                     /locus_tag="OO309_003944"
     CDS             2071..2898
                     /gene="tauC"
                     /locus_tag="OO309_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR5848886.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2895..3746
                     /gene="tauD"
                     /locus_tag="OO309_003945"
     CDS             2895..3746
                     /gene="tauD"
                     /locus_tag="OO309_003945"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR5848887.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3852..4826)
                     /gene="hemB"
                     /locus_tag="OO309_003946"
     CDS             complement(3852..4826)
                     /gene="hemB"
                     /locus_tag="OO309_003946"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR5848888.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT         1257 a         1340 c         1500 g         1321 t
ORIGIN      
        1 cgttgatgct cgttgatatg cccgacgagc aagtcagaga gattttggaa gagattgaag
       61 ctagtattct ctcagagagt gtaaacgcga aggcgggtta acttcattga cctgccttta
      121 gggtgccttt aaaattcgtt tttaaaggct acgatgacat ttttgaagag tcttaaaaga
      181 attaaaatac acggcgcgcc tatcttgaat gttttatcga tatcgaagca ttgagcattt
      241 ttaactcgtt ttatcttgaa tgcaatcggc gcataactta ttgaatccca tcaaatcccg
      301 tcttttttta cccattcccg gttatcttga ataaattacc tagtcatata acacacttct
      361 tgccgcactg gcattcatcg cttttcaggc acaggcggtg aacgtcaccg tggcgtatca
      421 aacctcagcc gaaccggcga aagtggctca ggccgacaac acctttgcta aagagagcgg
      481 ggcgagcgtg gactggcgta agtttgacag cggagccagc atcgtgcggg cgctggcttc
      541 tggcgacgtg caaatcggca acctcggttc cagcccatta gcggttgcag ccagccaaca
      601 ggtgccgatt gaagtcttct tgctggcgtc aaaactgggt aactccgaag cgctggtggt
      661 aaagaaaact atcagcaaac ccgaagattt gattggtaaa cgcattgccg taccgtttat
      721 ctccaccacc cactacagcc tgctggcggc gctgaaacac tgggggatta aaccagggct
      781 agtggagatt gtgaacctgc agccgcccgc gattatcgcc gcatggcagc ggggtgatat
      841 tgatggtgct tatgtctggg caccggcggt taacgccctg gaaaaagacg gcaaggtgct
      901 gaccgattct gaacaggtcg ggcagtgggg tgcgccaacg ctggacgtct gggtggtgcg
      961 caaagatttt gccgagaaac atcctgaggt cgtgaaagcg ttcgccaaaa gcgccatcga
     1021 tgcacagcaa ccgtacattg ctaaccctga cgcgtggctg aaacagccgg aaaacatcag
     1081 caaactggcg cgtttaagcg gcgtgcctga aggtgacgtt ccggggctgg tgaagggaaa
     1141 tacctatctg acgccgcagc aacaaacggc agaactgacc ggaccggtga acaaagcaat
     1201 catcgacacc gcgcagtttt tgaaagagca gggcaaggtc ccggctgtag cgaatgatta
     1261 cagccagtac gttacctcgc gcttcgtgca ataaaaggag gcgcagatgc tgcaaatctc
     1321 tcatctctat gccgattatg gcggcaaacc ggcactggaa gatatcaacc tgacgctgga
     1381 aagcggcgag ctactggtgg tgctggggcc gtccggctgc ggtaaaacca ccctgctgaa
     1441 tctgattgcc ggttttgtgc cttatcagca tggcagcatt caactggcgg gtaagcgtat
     1501 tgagggaccg ggagcagagc gtggcgtagt ttttcagaat gaagggctgc taccgtggcg
     1561 caatgtacag gacaacgtgg cgttcggcct gcaattggca ggtatagaga aaatgcagcg
     1621 actggaaatc gcgcaccaga tgctgaaaaa agtggggctg gaaggcgcag aaaaacgcta
     1681 tatctggcag ctttccgatg gtcaacgtca gcgggtgggg attgcccgcg cgctggcggc
     1741 gaatccccag ctgttattac tcgacgaacc gtttggtgcg ctggacgcct tcacccgcga
     1801 ccagatgcaa actctgctgc tgaaactctg gcaggagacg ggcaagcagg tgctgttgat
     1861 tacccacgat atagaagaag cggtgtttat ggcgactgaa ctggttctgc tttcatccgg
     1921 ccctggccgt gtgctggagc ggctgcggct caactttgct cgccgctttg ttgcgggaga
     1981 gtcgagccgc agcatcaagt ccgatccaca attcatcgcc atgcgcgaat atgttttaag
     2041 ccgcgtattt gagcaacggg aggcgttctc atgagtgtgc tcattaatga aaaactgcat
     2101 tcgcggcggc tgaaatggcg ctggccgctc tcgcgtcagg tgaccttaag cattggcacg
     2161 ttagcggttt tactcactgt atggtggacg gtggcggcgc tgcaactgat tagcccgctg
     2221 tttttgccgc cgccgcaaca agtactggca aaactgctca ccattgccgg accgcaggga
     2281 tttatggacg ccacgctgtg gcagcatctg gcagccagcc tgacgcgcat tgtgctggcg
     2341 ctactcgcgg cggtggtgat cggtattccg gtcgggatcg ccatggggct aagcccgacg
     2401 gtgcgcggca ttctggatcc ggtaatcgaa ctttatcgtc cggtgccacc gctggcttat
     2461 ttgccgctga tggtgatctg gtttggtatt ggtgaaacct cgaagatctt actgatctat
     2521 ttagcgattt ttgcgccggt ggcgatgtcg gcgctggcag gggtgaaaag cgcgcagcag
     2581 gttcgcattc gtgccgccca gtcgctgggt gccagccgtg cgcaggtgct gtggtttgtc
     2641 attttgcccg gcgcgctgcc ggaaatcctc accggattac gcattggtct gggtgtgggc
     2701 tggtctacgc tggtagcggc ggagctgatt gccgcgacac gcggtttagg atttatggtt
     2761 cagtcagcgg gtgaatttct cgcgactgac gtggtactgg cggggatcgc ggtgattgcg
     2821 attatcgcct ttcttttaga actgggtctg cgcgcgttac agcgccgtct gacgccctgg
     2881 catggagaag tacaatgagt gaacgtctga gcattacccc gctggggccg tatatcggcg
     2941 cacaaatttc gggtgccgac ctgacgcgcc cgttaagcga taatcagttt gaacagcttt
     3001 accatgcggt gctgcgccat caggtggtat ttctgcgcga tcaagctatt acgccgcagc
     3061 agcaacgcgc gctggcccag cgttttggcg aactgcatat tcaccctgtt taccctcatg
     3121 ccgaaggggt tgacgagatc atcgtgctgg atacccataa cgataatccg ccggataacg
     3181 acaactggca taccgatgtg acatttattg aaacgccacc cgcaggggcg attctggcag
     3241 ctaaagagtt accttcgacc ggcggtgata cgctctggac cagcggtatt gcggcctatg
     3301 aggcgctctc tgttcccttc cgccagctgc tgagctggct gcgtgcggag catgatttcc
     3361 gtaaatcatt cccggaatac aaataccgca aaaccgagga ggaacatcaa cgctggcgcg
     3421 aggcggtcgc gaaaaatcct ccgttgctac atccggtggt gcgaacgcat ccggtgagcg
     3481 gtaaacaggc gctgtttgtg aatgaaggct ttactacgcg aattgtcgat gtcagcgaga
     3541 aagagagcga agccttgtta ggctttttat tcgcccatat cactaaaccg gagtttcagg
     3601 tgcgttggcg ctggcaaccg aatgatattg cgatttggga taaccgcgtg actcagcact
     3661 atgccaatgc cgattacctg ccacagcggc ggattatgca tcgggcgacg atccttgggg
     3721 ataaaccgtt ttatcgggcg gggtgatacg agtgcggacg gttccctcgc ccccttgggg
     3781 agagggttag ggtgaggggg cgttcactgt actttcaaca ggttaactcc ccctttctga
     3841 gaggaaaaaa attaacgcag aatcttcttc tcagccaaat ccagcgcaaa gtagctgaaa
     3901 atcagatccg cacccgcacg cttaattgaa cctaagcttt cgagcacgac tttctcttca
     3961 tctatagcac ccgccagcgc ggcgaactta atcatcgcgt actcaccgct cacctgatac
     4021 gcgccaatcg gcaattcagt acgttcacgc agctcacgca cgatgtcgag gtacgctcca
     4081 gcaggtttaa ccatcaggca gtctgcgccc tgggcttcat ccagcagtga ctcacgaatc
     4141 gcctcgcgac ggttcattgg gttcatctga tagcttttgc ggtcgccttt taatgcgctt
     4201 cctgcagctt cacggaacgg gccataaaag gaagaggcga acttggtcga atacgacata
     4261 atcgccgtat ctttaaagcc tgcagcgtcc agcgcctgac gaatcgcctg tacctggccg
     4321 tccatcgcgg cggaaggggc gatgaagtct gcgcctgcag cagctgcaac cacggcttgc
     4381 ttgcctaaat tttccagagt cgcgtcgttg tcgacgccat gctcgcacag cacaccgcag
     4441 tgaccgtgag aagtgtattc acagaaacaa gtgtctgaca tgacgatcat ttctggcaca
     4501 gtctgcttgc agatgcgcga catacgcgcc accagtccat cttcccgcca ggcgtcgctg
     4561 ccggtttcat cggtatggtg agagatgcca aaagtcatta cggaacggat accggcgttg
     4621 gcgatgcgtt caatttcgcg tgccagatgt ttctctggaa tgcgcatcac gcctggcatg
     4681 gcttcaacgg ctttgtagtc gtcaatttct tcttcaacaa agatcggcaa caccaggtcg
     4741 ttaaggctaa gtgttgtctc ttcaaacata gcgcgcagcg caggagattt gcgcaggcga
     4801 cgagggcgtt ggattaagtc tgtcatggtc tgcctgatgt ttgtggaatt aaggtgcaca
     4861 gtatacctga agcggggcag gagtgtttta cgaaagttgt cgcgatgttg acaagaagag
     4921 aatgaaagag agaccaggac atctttgtaa tagaaatagc aataatttca tattcactga
     4981 aaatattttt aatctttatt tatagctctt ggttattatt ttttggcgtt tggttgtgct
     5041 gctataaatt gaataattca aactttgttg tatattgata agatatttat ctggtttgta
     5101 tttttgatat ttataacgcg tatgcatcaa aaataacatt atctgcaaca tactgaaata
     5161 tcgtcctttt atcaatttga tgcgtgtttt tctccataaa ttatatatgt ccacattcgg
     5221 acttagggga aagaataatt gaaccattcg tctgtaacgc agcataatcg ttagcgcgaa
     5281 acataatatg ttttctatgc attgataatt gatggatcaa cttattacgt ccctgaggag
     5341 ggatgacaaa tgcactcctg gaaaaagaaa cttgtagtat cacaattagc attggcttgc
     5401 actctggcta tcacctct
//
LOCUS       DAJTQQ010000175         5398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000175 DAJTQQ010000000
VERSION     DAJTQQ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.177"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..497
                     /locus_tag="OO309_003947"
     CDS             <1..497
                     /locus_tag="OO309_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HCR5848889.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="OO309_003948"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="OO309_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="OO309_003949"
     CDS             2500..3114
                     /locus_tag="OO309_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR5848890.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="OO309_003950"
     CDS             3114..3443
                     /locus_tag="OO309_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR5848891.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="OO309_003951"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="OO309_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR5848892.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4529..4840
                     /locus_tag="OO309_003952"
     CDS             4529..4840
                     /locus_tag="OO309_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5848893.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4824..4982
                     /locus_tag="OO309_003953"
     CDS             4824..4982
                     /locus_tag="OO309_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5848894.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAT
                     RIAKEKCA"
     gene            4975..5373
                     /locus_tag="OO309_003954"
     CDS             4975..5373
                     /locus_tag="OO309_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5848895.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1178 a         1344 c         1424 g         1452 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtggcgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 ttttattgtc tgagatcaga gatacggcta ataaccgtat ctcttgacag agtgagcaga
     4441 tgcacataaa ttaatcatac ggttgatagc cgtattatgt atgagatcga ctccctacga
     4501 tttcattcag ttaatcaaag aagtcataat gcctggaaaa acagccgctc tatacgatgt
     4561 cgataaaacg ctgaaaaatg cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa
     4621 acttcgtgaa gctgcgcaag ccgttggggt agatttaagc gcttttattt tgagcgctgc
     4681 tatggaacgc gccgaaagtg tacttgataa ccaacgtcgc cgtgagcttt cgaatcaaag
     4741 ctgggaactg atgaaccaac tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc
     4801 gttaatgaaa aggaaaaaca gcgatggtcg acaagcatga agagattact ctgcccatag
     4861 tcctctcctg taattatcag tctgatatta cttatcctgg gcaaaaacag tttgattgcg
     4921 gtaaccctgt tatcgataaa tttgtacgcg ctacgcgcat cgctaaagaa aagtgtgcgt
     4981 aatagcgact gtgcggctaa agcacttatt gacagacaaa gtggtgaact gattggcatc
     5041 tgtactttta cggcatattc gctggaaaga caacgcgttt ctggcgtcct tcagggttca
     5101 caaccttcag aaattggtgt tgtcagatta gtcatgttgg ggggagcgcg gaagtatcaa
     5161 aagcggggct ttggtcagga cctactatgt gatttttttg aacatgtaaa aaaaattcac
     5221 ccggcattac caattaaagg ggtttatctt gatgctgacc ctgccgccat taacttttat
     5281 gctcgtctcg gctttgttca gctttcagcg acaccaaatg cttttggtgc tgtacctatg
     5341 tttttggcga ttcagcatat tctcgcggct tagattatta tcgccacccg ccagggca
//
LOCUS       DAJTQQ010000176         5389 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000176 DAJTQQ010000000
VERSION     DAJTQQ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.178"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..1630)
                     /locus_tag="OO309_003955"
     CDS             complement(<1..1630)
                     /locus_tag="OO309_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCR5848896.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPW"
     gene            complement(1646..2530)
                     /locus_tag="OO309_003956"
     CDS             complement(1646..2530)
                     /locus_tag="OO309_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5848897.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(2541..3146)
                     /locus_tag="OO309_003957"
     CDS             complement(2541..3146)
                     /locus_tag="OO309_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5848898.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3317..>5389)
                     /locus_tag="OO309_003958"
     CDS             complement(3317..>5389)
                     /locus_tag="OO309_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HCR5848899.1"
                     /translation="VEGNIESNQKLIVLGNLTVKGNISTFSLSNPWVILGNVTATNIV
                     TDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGVFINDIIAPVVASSTNSEFMV
                     RASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIFKRSNIRMAGNVCNQMKKEAL
                     FRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMRTLPRLKGISANLRKQLINSN
                     DEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDREPLSVEIMTRMSRLPDGVGPL
                     NLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPESIIDGYIRSDDSSIRQVGAGG
                     QLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRMTPQESDKVAAYLYQKFENDD
                     DLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVIHSDAALQRLLTRFNDPEGWS
                     NLAKNQYLSTSMKQKIWQRALSHRKNNPKADSDAYETSADMILSELISHGEVDDQMLL
                     NAAALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPRNDIWAKFFLRQENSSRAQVD
                     EALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLTFFKFGALSNRHTPSAALAAE
                     IDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPELDLVRQLALNGKTRAIREQA
                     MRKLDELY"
BASE COUNT         1555 a         1341 c         1121 g         1372 t
ORIGIN      
        1 cccacggttt accatcttta tgactggtta acaccccggc aatcagctcc agttgtgggt
       61 gccacggctg cccggtgtag atgagaatgc cacccggttc gatggcattg gctagacctg
      121 cgagtgagtt ttttacctgc tcattttcgg gaaaaagctc atataggcca gagacaatcg
      181 ccagcgtagg ccgcggagtt aacgcgctga gttcctccgg gttaaacgca tcgccctgtt
      241 caaaacgcac ccgcccagac attccccgtt gggcaatcat ctcttgcccc tgtgcaacat
      301 ttaactcact gtaatcacgt aacaaaatat cgcttacggc aggctcgttt gccagcgcat
      361 ccagtacata gcgtccatgc cctgcggcaa tgtcaaccac gcggacggct aaacctttgg
      421 cgtggagatc ggcaacggct tgtttaatca atatttgcaa atgggttttg cgctggcgaa
      481 taccgcgcca tccaacactg ttcaggtaga ttttgtcgat taaacgcccg aatgcgttac
      541 tgccttgcgg ttgattttga tagacataat ccagggaact gcctgaatca aagccggtac
      601 tcattccgag atgcaggccc gcagagtgcg caccgaacaa tttcatcgcc tttcgcataa
      661 agcgatacgc taaatcaacc ggcgataatg gcacgggtcc accagaaagg agccgccatc
      721 gatccgctga tggtcctgtg cggtcttcat gctgataatc aaatttttgc gatttgttag
      781 catataagcg gcagataaag ctttgcattt tttcaaatgc ctgcgccctg ttctcttcac
      841 ccaatgtgtc gtgataaaag cctggcagca gatgcagctc tttcagaggg ctacgtaatc
      901 tctgataaaa atcaatctgc ggttggcgat gcaccacata gtcatcacct gatatcagaa
      961 gttgcgtggg gagcgtaatc gccgccgcat cgctaacaat acgttcagac gttttataga
     1021 gatcgagcaa gatattgacg gcaatcgccc gtgtgatcag cggatcatta ttgaaactcg
     1081 ccacccgttg ccgatcgtgg gtcaaatagc gccctttcac ataggaatta ataaaaaata
     1141 gaccacgcag acgatgccat aacgccagcg caggacgtgc cagcggcaca tacaatttaa
     1201 ccttaaaggc cggagaagcc agcaccagcc cacgaattgc aggtgcataa tcatgaaccc
     1261 atgtggcaac aagcactgcg ccgacgcttt gcgcgatcac taccacctct tccagtccga
     1321 cctggctgtc gctggcagca aaacggacaa attcatcgac atcctgcact gagcgcataa
     1381 gagacgggct ataaccacgc ggccccgaat tttttccatg cccccgggca tcccaggcat
     1441 aaaaagcagt atcaggcatc gccagttcat caacgagatg ttgtagacga ccagaatgtt
     1501 catgcccgcg atgaaataag acgatgacct ttttcgcccc tggctgtaaa gtgggccagt
     1561 gccgataata caatgctgta ttatcactgg tggtaaaaaa atgttcccca gggatgcgtg
     1621 aattttccat tttctctatt ttccttcagt aacagcagta gcgaataaaa taaaaaaata
     1681 ccggagcggt gaaaattaat gagtcaatcc tgtcaaggag tccgccatgt cctagtaata
     1741 gttttccact atctttaaca ccaatatctc gtttgatggc tgacatcact acatcgccgc
     1801 aaaaaccact aataccaatt aacaaacccg ctaataatgc ctgtaatgta tttagcggtg
     1861 tcagcaacgg tccaataatc agtgacgcaa tcatggtggt gattacgccc cccagcagac
     1921 cttccagcgt ttttcccggg ctgactttgg ggaccacttt tcttctgccg caggatttcc
     1981 cccataaata ctgtgcaata tcgtttgact cggttaacgc caaaagaaat aacaccagta
     2041 acgccccgcc ctggatattt atggttggca acattaatag ccaggcggca tgactcaagg
     2101 caaaaacggt cattatccat ccccaaaaaa tggcgctcac ggtatgctga aaaccggagg
     2161 ggtcacccac aaaaactcgc caggtggcta atatcagaaa cccagtcaaa ggtataaata
     2221 acagaaacag ctcaaagcaa ttaaaaccga taagcaaata gttaagagga ataacccaat
     2281 ataaccaacg cgaaaaatgt acggatataa gcgtacaata ttctttcaat gccagaaaac
     2341 ttatcagcgt aaaaaacgtc aacgtcatcc agcgtgggcc tgaaatcacc agtgaaaaac
     2401 aaatgacgat gccccaccag gtacgaatac gtaatgtaac ttcatcccct tttctctcag
     2461 gtaaccgcca tagcagaaaa cggtttatca gcttgctaaa attaacaacg cgaaaagtgt
     2521 tgccagagat ttttccagca ttatctttca gcgctcataa gaagcacact ccgacaacga
     2581 ttaatcgcag tacagataag cagaattgaa gcaatgctcc ataaaagatt attccattgc
     2641 atccactgtg gataaatggc aacggccaga ccccacagac caaatattaa cgcgcgatca
     2701 cttttgccaa atggaccggc ataactgcga acaccgttaa tcgtctgcgc gagtaaaccg
     2761 caaaactcgg tcaatatggt gcaaaataac atgagtatca cgagtgatgc gttactttcc
     2821 ggtaaaaata aaaacggtaa atagagcgca atatcggaaa taacatcgcc agtctcattc
     2881 aaaatcgccc ccagacgtgt ttgctgattg cactcacgcg ccaacatgcc atccagcgca
     2941 ttgagcgcca tacggataaa aagcacgatg ggcaatagca aaaagaggat gggttgtgcc
     3001 accaacacca acagcaatcc ggtaaaaaga gaaagcgcca gtgccgtaag agtgatgtga
     3061 ttcgctgtaa cgtggtgctt ataaagccaa aacatcgtcg gccttaacag cgactgaaag
     3121 agcggtttta tctggtagag cgtcattatc aatccttgat aattcggttt gcgctatggt
     3181 gcatcaattc ccctgacgga gatatcgcaa cacgccgctt cattaaaaat tcagaatata
     3241 tcaaggtatt gggggctcac tgtcaggaat gattatcccc aataaatcaa gtagttaaaa
     3301 attattacgc tgttattcaa tacaactcat caagtttcct catcgcttgt tcccgtattg
     3361 cgcgcgtctt accgttaagc gccagctggc ggactaaatc cagttcaggg ttaacaagct
     3421 ctaacgccag caaaggatca cgtttcagcc tcgccttcaa tacatcaact ggaaagcggg
     3481 gattattcat cgccacaatc caccactcgg gatcaatttc tgccgccagt gccgcagagg
     3541 gggtgtggcg gttgctaagt gcgccgaact tgaaaaatgt gagattgctt ttcgccagtg
     3601 tactccacca tatacgatcc ggttgttttg ccagtacatc cagttgtgct aacgcatcgg
     3661 ggtcgagtgc ataataaaca cgtaatgctt catcaacctg cgcacgggag ctattttcct
     3721 gcctcagaaa aaacttcgcc caaatatcat tgcgtggcgt gttttgctgt aattccttga
     3781 gaacaacagc gggtaaatta tcccaactaa ctaatgcact ctctaaaaag tcccagtctt
     3841 ccaaacgtat cagggcggcg gcatttaata gcatctggtc atcaacttcg ccgtggctaa
     3901 tcagctcaga caaaatcata tcagcgctgg tttcatatgc atctgaatct gctttcggat
     3961 tatttttccg atgcgataaa gcacgttgcc aaattttttg tttcatcgac gtgctcagat
     4021 actgattttt cgccagatta ctccacccct caggatcgtt gaaacgtgtc aataaccgtt
     4081 gtaaagcagc gtcagagtga attacctgca tatcccgaca gcaaaagtag gcaggggatt
     4141 ttttctccat ccttttaaca aaattaaact gtaagttatc tggcaatgct aaaaataaca
     4201 cacgtattag atcatcgtca ttctcgaatt tttggtataa ataggctgca actttgtcac
     4261 tctcttgtgg cgtcatccgc aataattgac catggtgttt catctctgcc agcttgaacg
     4321 ctaagcttgt gacaacattg tttgatgaat cgttcgccaa ctgcattgcc tgattgtaag
     4381 tgagttgtcc tccagcaccg acctgacgaa tagaggaatc atcactgcgg atatagccat
     4441 caataatgga ttcgggcaat aaccacgcat ctttataaag ctcctgaatg atcatatccc
     4501 aatctctttt cgccagagtc atgacgatat ccagagggag attttcacgt agcgcaagat
     4561 tcaacggacc gacaccatca ggtagacgac tcattcgcgt cattatttcg acagagagcg
     4621 gttcacggtc gagcaacccc agaacaacag gctggtaacc caattgctga tcggtcaact
     4681 ccagaatctc attgtcaggc atatatctcg ccatactttc aatcgtttgt tcatcattgc
     4741 tattaataag ttgtttgcgt aggttagctg aaattccttt aagccttggg agagttcgca
     4801 ttatggcaag atcaaatgtc gcaatatctc gtccttcaaa tgcagcaacg ttgccttcat
     4861 ccagcatttg caattcctgc actaactcag gagaaggctt agggcgaaac agcgcttctt
     4921 ttttcatctg attacagaca ttccctgcca tgcgaatatt gcttcgctta aaaatttctt
     4981 tcagagcatc ttcatcattt attagacccc aataatatgc atcaggattt attatcatca
     5041 gcgctttttt gacattttca gtgtcatttt tgtcactcgc acggaccatg aattcactat
     5101 ttgtcgaaga cgcaacaaca ggtgcaatta tgtcattgat aaatacccca cgcgcattaa
     5161 tactcccctt aatcgtagac ggattatcgt aatatgagtc gataaatacc agtccacttg
     5221 catttatcga accggtaatt aacagtggtg aatccgtaac tatattcgtt gccgtcacgt
     5281 taccgagaat aacccatgga ttagaaagag agaaagtgga aatattacct ttgactgtca
     5341 aattgccaag aacaatcaat ttttgattac tctctatatt accttctac
//
LOCUS       DAJTQQ010000177         5374 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000177 DAJTQQ010000000
VERSION     DAJTQQ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.179"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            433..1341
                     /locus_tag="OO309_003959"
     CDS             433..1341
                     /locus_tag="OO309_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR5848900.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1387..3378)
                     /gene="tkt"
                     /locus_tag="OO309_003960"
     CDS             complement(1387..3378)
                     /gene="tkt"
                     /locus_tag="OO309_003960"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5848901.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFEAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3656..4414
                     /gene="loiP"
                     /locus_tag="OO309_003961"
                     /gene_synonym="yggG"
     CDS             3656..4414
                     /gene="loiP"
                     /locus_tag="OO309_003961"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR5848902.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4620..>5374)
                     /gene="speB"
                     /locus_tag="OO309_003962"
     CDS             complement(4620..>5374)
                     /gene="speB"
                     /locus_tag="OO309_003962"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR5848903.1"
                     /translation="AAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDARE
                     MSEKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANG
                     CEFDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQV
                     KQIVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVE
                     VAPAYDQSEITALAAATLALEMLYIQAAKKGE"
BASE COUNT         1315 a         1405 c         1389 g         1265 t
ORIGIN      
        1 ttgctgaatg tataactgag attgatgatt ctggaaaata atcactaagc cgcataaatt
       61 atccttattt tatttttagg gtaccgtccg gcggtaaaaa taaaatactc gtgcggttta
      121 atgaaagcaa ttgggtacgc gagaagaaaa accccacaaa ccaaaataat aaaaccttaa
      181 ataacaacaa tatgaaattg atatttttaa agtcaacttt gtatttgtta tgttcatcac
      241 aaaatcaaca ttttgtttgt tattgtattt gtgatgcata tctctattta tttattgaat
      301 gttcgaaaac atacttttct aatgcaatgt tttttcgcta taccccttta tagattctga
      361 attctcacct gtcggctgtt ttctcctcat ctcatgtgac atattcccat actttactca
      421 aggaagattg tcatgaaagc acctgtttca ttaacctcat ttcgcccaca aaaatctctt
      481 gctatcgcca ttggcgtgct tgccgtggtt gtgctgccat ttctctcgta ttacaccgtt
      541 aatgaaggtg aacgcggcat tttgctgcgc tacggaaaaa tcgtaaaagt tgccgaaccg
      601 gggctgggtt ttaagatccc ttttatggaa agcgtggaga aaatttccac gcgtaatcag
      661 gctgtggtct atcagggatt acaggcctac agccgcgacc agcaaccggc acaaatgaca
      721 gtatcagtca gcttccacat taaaccttca gaagcaggcg cggtttatac cacctacaac
      781 actattgaag cattaaaaga tcgtctaatt gtccgtcagt tgccaactca gctggagaac
      841 attttcggcc aatataccgc catcagcgcc gtacaggatc gtacaaaatt ggttcaggat
      901 ctgcaaaatg ccatgcgcaa agccgttgtt gggccagtgg taattgacgg ggtccagatt
      961 gaaaatattg atttttctga cgcttatgaa aagtcgattg aaaaccgaat gaaagcagaa
     1021 gtcgccattg ccacgcgtaa gcaaaatctg gaaacagaga aaatccaggc gcaaatagcg
     1081 gtaactcagg cccaggcaga agcagacagt aaactggcgg cagccaaagc agaagcggaa
     1141 actattcgcg taagaggcgc ggcagaggct gaaactattc gtttaaaaag tgcggctgaa
     1201 gcagaagcaa tccgcctgcg cggtgaagct ctgcgggata atcccggact tgtagccctc
     1261 accactgccg aacgttggga cggtaaactg cccgatacca tgatccccgg cagcacagtt
     1321 ccgtttatct ctactaagta aaaaaagggc cgctttcgcg gtccttctca caaaacgtct
     1381 tactgattac agcagcgctt ttgctttcgc aacaacgtta tcaacagtga agccgaactc
     1441 ttcaaacagc agctctgccg gagcagattc accgaaggtg gtcatgccga cgatagcgcc
     1501 gttcaggcca acatacttgt accagtagtc agcaataccc gcttctaccg caacgcgtgc
     1561 agtaaccgct ttcggcagta cggattcacg gtaagccgca tcctgcttgt caaatgcgtc
     1621 ggtagacggc atggacacca cgcgcgcttt cacgccttcg gcagtcagtt tttcgtaggc
     1681 agcaaccgcc agttcaactt cagaaccggt ggcgatgaag atcagttccg gctgaccagc
     1741 acagtctttc agcacataac caccgcgcgc gatgtttgcc agctgctctt cagtacgttc
     1801 ctgctgcgcc aggttctgac gggagaggat aagcgcagtc gggccgtcct gacgctcaac
     1861 gccgtatttc cacgcgaccg cggattcaac ctggtcacac ggacgccatg tagacatgtt
     1921 cggggtcacg cgcagagaag cgacctgctc taccggctgg tgagtcgggc catcttcgcc
     1981 cagaccgatg gagtcgtggg tgtaaaccat cacctggcgc tgtttcatca gcgcagccat
     2041 acgtacggcg ttacgtgcgt attccacgaa catcaggaag gtggaggtgt acggcaggaa
     2101 gccaccgtgc agggagatac cgttagcaat cgcggtcata ccgaactcgc gaacaccgta
     2161 gtggatgtag ttacctgcag catcttcgtt gattgcttta gaaccagacc acagggtcag
     2221 gttagacggt gccaggtcag cagagccgcc gaggaattca ggcaacagcg ggccgaacgc
     2281 ttcgatagca ttctgcgacg ctttacggct ggcgattttc gccggattag cctgcagttt
     2341 agcgataaac tctttcgctt tggcgtcgaa gtcagacggc atttcgcctt tcatacggcg
     2401 ggtaaattca gccgcttcct gcggataagc tttcgcgtaa gcctcaaact tctcattcca
     2461 tgcagattct ttcgcctggc ctgcttcttt cgcatcccac tgagcataga tttcagacgg
     2521 gatttcgaac ggtgcatatt tccagcccag ctgttcgcgg gtcagggcaa tttcagcgtc
     2581 gcccagcggc gcaccgtggg agtcgtgagt accggctttg ttcggggaac cgaaaccgat
     2641 gatggttttg cacatcagca gggacggttt gtcagtcact gcgcgcgctt cttcaactgc
     2701 gcgtttgatg gatgccgcgt catgaccgtc gatgtcgcga ataacgtgcc agccgtaagc
     2761 ttcgaaacgc attgctgtgt cgtcggtgaa ccagccttca acgtgaccat cgatagaaat
     2821 accgttgtca tcgtagaacg caatcagttt acccagcttc agcgtacccg ccagagagca
     2881 aacttcgtgg gagatgcctt ccatcatgca gccgtcgccc atgaaggcgt aggtgtagtg
     2941 gtcgacaatg tcgtggcccg gacggttaaa ctgcgccgcc agcgtttttt ctgcaatcgc
     3001 cataccgact gcgttggcaa taccctgacc cagcggaccg gtggtggttt ccacaccagc
     3061 ggtataacct acttccggat ggcccggagt tttagagtgc agctgacgga agtttttcag
     3121 ttcttccatc ggcagatcgt aaccggtgag gtgcagcagg ctgtagatca gcatggagcc
     3181 gtggccgttg gacagcacga agcggtcacg gtcagcccag gacggattct gcgggttgtg
     3241 tttcaggaaa tcacgccaca ggacttcggc aatgtcagcc atacccatag gggcacccgg
     3301 gtgaccggat ttggctttct gtactgcgtc catgctcagc gcacgaatag cattggcaag
     3361 ctctttacgt gaggacattt tgactccaga tcggatgatg aagggcacgc ccttaacgac
     3421 ttgacgacag cgcgttttgg gctacgccgg aaaatttgcc aacaatttac cgcaagccgc
     3481 gcgtcatgta catggaacat ccttttgccg cttcagaaat ctctggatca tgctcgcatg
     3541 ttgcgcaatc tgctcgcccg tccgctgcgc ttttccttat actgagactg agcgtcgatt
     3601 cacctgcaaa cggcgcattt ttagaataat cctgaccttg tgcggaagag aaaacatgaa
     3661 aattcgcgcc ttattggtag caatgagcgt ggcaacggta ctgaccggtt gccagaatat
     3721 ggactccaac ggactgctct catcaggagc ggaagctttt caggcttaca gtttgagtga
     3781 tgcgcaggtg aaaaccctga gcgatcaggc atgtcaggag atggacagca aggcgacgat
     3841 tgcgccagcc aatagcgaat acgctaaacg tctgacaact attgccaatg cgctaggcaa
     3901 caatatcaac ggtcagccgg tgaattacaa agtgtatatg gcgaaggatg tgaacgcctt
     3961 tgcaatggct aacggctgta tccgcgtcta tagcgggctg atggatatga tgacagataa
     4021 cgaagtcgaa gcggtgatcg gtcacgagat ggggcacgtg gcgttaggcc atgtgaagaa
     4081 agggatgcag gtggcgcttg gtacaaatgc cgtgcgagta gctgcggcct ctgcgggcgg
     4141 gattgtcggc agtttatctc aatcacaact tggtgatctg ggcgagaaat tagtcaattc
     4201 gcaattctcc cagcgccagg aagcagaagc cgatgattat tcttacgatc ttctgcacca
     4261 acgcggcatc agcccggcag gtcttgccac cagctttgaa aaactggcga agctggaaga
     4321 aggtcgtcaa agctcaatgt ttgacgacca tcctgcatcc gccgaacgtg cccagcatat
     4381 tcgcgatcgc atcagcgcgg atgggattaa gtaaagccta gtggtgtcga acgtaggtca
     4441 gataaggcgt tcacgccgca tctgacatta atggcacatt tgttaccttg tgcgcattgc
     4501 cggatgcgat gctggcgcat cttatccggc ctacgggtgc cgaacgtagg tcggataagg
     4561 cgttcacgcc gcatccgaca ttaatggcac gttttacccg tgcgcatcgc atctggtgct
     4621 tactcgccct ttttcgccgc ctgaatatac agcatctcca gcgccagcgt cgccgcagcc
     4681 agagcggtga tttccgactg atcgtatgcc ggagccactt ccactacgtc catcccaacg
     4741 atgttgagat ctttcaggcc gcgtaccagt ttaatggcgc gatcggaggt caggccgcca
     4801 atcactggcg taccggttcc cggcgcgaaa gcaggatcca ggcagtcgat atcaaaagtc
     4861 aggtaaaccg gcatatcacc cacaatctgt ttcacctggg cgataacgtc atccacgctg
     4921 cgatcgttca cctggcaggc gtccagcacg gtaaagccgt tgtctttatc aaactcggta
     4981 cgaataccaa tctgcacgga atgattcggg tcgatcagac cttctttcgg cgcggtatag
     5041 aacatagtgc cgtggtcaaa ttcacaaccg ttcgcatagg tatcggtgtg ggcgtcaaag
     5101 tgtaccagcg ccattttgcc gaaatgcttc gcatgagcac gcagcagcgg cagcgtaaca
     5161 aagtggtcac caccgaagga gagcatacgc ttaccggcag ccagcagctt ctcggcgtgc
     5221 gcctgcagct tttcgctcat ctcacgggca tcgccaaagg catataccag atcgccgcag
     5281 tccacgacgt tcagacgctc acgcatgtcg aaattccacg ggaagcggtt gtgttcccag
     5341 gccagattcg tcgaaacctg acggatcgct gccg
//
LOCUS       DAJTQQ010000178         5364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000178 DAJTQQ010000000
VERSION     DAJTQQ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.180"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(63..800)
                     /gene="bamD"
                     /locus_tag="OO309_003963"
     CDS             complement(63..800)
                     /gene="bamD"
                     /locus_tag="OO309_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR5848904.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            935..1915
                     /gene="rluD"
                     /locus_tag="OO309_003964"
     CDS             935..1915
                     /gene="rluD"
                     /locus_tag="OO309_003964"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR5848905.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1912..2643
                     /gene="pgeF"
                     /locus_tag="OO309_003965"
     CDS             1912..2643
                     /gene="pgeF"
                     /locus_tag="OO309_003965"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR5848906.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2773..5346
                     /gene="clpB"
                     /locus_tag="OO309_003966"
     CDS             2773..5346
                     /gene="clpB"
                     /locus_tag="OO309_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR5848907.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1317 a         1296 c         1460 g         1291 t
ORIGIN      
        1 tcagttgctt aactgactgg cattacagct caagggctgc cgttttgtgt ttcaggtttc
       61 tgttatgtat tgctgctgtt tgcggcgatg attttcgcta ctttttcagc ttgcgcattc
      121 atctgcatct gacggtatgc attttccatc agcggcagcg catcacgcgt agcctgggta
      181 tccgggtagt cgcgcaacat gccttctatg cggttaacga cggcaaccca tgcgccacgt
      241 tctgtatagt actcggccac ggagtattca tatttcgcca gacgatcttt caggaatacc
      301 agacgtttgg tggcatcggt agtgtactga ctgttcggat agccgcgcac cagtttggaa
      361 aagtcactaa acgcagctcg tgcatgttga ggatcgcgat cgctacggtc aacgccaaag
      421 aacccttgca gcgcactgtc atccagcgcc atattggtca ggccacgcat gtacatgaca
      481 taatcgatat tcggatgggt cgggttaagg cgaataaaac gatcgatggc agcctgcgct
      541 aacggcaaat cggcgttttt atagtaggcg tagatgagat ccagctgcac ctgctgcgaa
      601 tacggaccaa acggatagcg attatctaac gcttccagtt gcgttattgc ctgtctccag
      661 ttaccgtcct gcagcttttg ttgtgcagtc gcgtaaattt catttggcgg attatcaggt
      721 acttcttcct ttgaccccga gcaacccgcc aaaaacaggc ttagtgtggc ggctgccacc
      781 agatatttca tgcgcgtcat gacgttttga ctttcctcaa aatgtaatac gggagattct
      841 ctgttcctgc acccggttaa gaccagctac aatagcacac tatattaaac ggcaaagccg
      901 taaaacccca acgataaacg aagaagcagt atatatggca caacgagtac agctcactgc
      961 aacggtgtcc gaaaaccaac tcggtcaacg cttagatcag gctttggccg aaatgttccc
     1021 ggattattca cgttcgcgaa taaaagaatg gatcctcgac cagcgcgtgc tggtaaacgg
     1081 caaagtttgt gataagccga aagaaaaagt attgggtggc gagcaggttg ccatcaacgc
     1141 tgagattgaa gaagaagcgc gttttgaacc gcaggatatc cctctggata tcgtctatga
     1201 agatgaagat atcatcgtca ttaataaacc gcgcgaccta gtggtacatc ctggcgcggg
     1261 taacccggat ggcacggtac tgaatgcgtt gcttcattac tatccgccca ttgccgatgt
     1321 accacgtgcg ggcatcgtcc atcgtctgga taaagacacc actggcctga tggttgtggc
     1381 aaaaaccgtt ccggctcaga cgcgtttagt cgaatctttg caacggcgtg aaattactcg
     1441 tgagtatgaa gcggtggcga ttggtcatat gaccgctggc ggtacagtag atgaacccat
     1501 cagccgccac ccgaccaaac gtactcacat ggcggtgcat ccgatgggca aaccggcggt
     1561 gactcactat cgcatcatgg aacacttccg tgtgcacacg cgtctgcggt tgcgtctgga
     1621 aactggacgt acgcaccaga tccgcgtgca tatggcccat atcactcatc cgctggtggg
     1681 cgatccggtt tatggtggcc gtccgcgtcc gccaaaaggt gcttcggaag catttatctc
     1741 cacgctgcgt aagtttgacc gccaggcgct tcatgcaacc atgctgcgtc tttatcaccc
     1801 gatctccggc atcgaaatgg aatggcatgc gcctattcca caagatatgg tggagctgat
     1861 tgaggtgatg cgcgccgatt tcgaagagca taaggatgaa gtggactggt tatgagtaag
     1921 ctgattgtcc cgcagtggcc gctgccaaaa ggtgttgcgg cctgtagctc cactcgtatc
     1981 ggcggcgtga gcttgccccc gtatgactca ctcaacctcg gtgcccattg tggcgataac
     2041 ccggatcacg ttgaggagaa tcgcaagcga ctttttgctg cgggcaattt gccttctaaa
     2101 ccggtctggc ttgagcaggt acacggcaaa gatgtgctta agcttactgg cgaaccttat
     2161 gcctcaaaac gagcagatgc ctcttatagc aatacgcctg gcacggtttg cgcagtgatg
     2221 actgccgact gcctccctgt gctgttttgc aatcgagcgg gaacggaagt cgccgccgtt
     2281 catgctggct ggcgtggact gtgcgcaggc gtgctggaag agacggtttc ctgttttgct
     2341 gataaacctg aaaacattct cgcctggtta gggccggcaa ttggtccacg cgcgttcgaa
     2401 gtgggggcgg aggttcgcga ggcgtttatg gcagtagacg ctaaagcaag tgcagctttc
     2461 attcagcatg gtgataagta tctggcggat atttatcagc ttgcccggca gcgtctggcg
     2521 aacgtgggtg ttgagcaaat tttcggcggc gaccgttgta catatacgga aaatgagact
     2581 ttcttctctt atcgtcgcga caagaccacc ggtcgtatgg caagtttcat ttggctgata
     2641 taacctaaag aatcaagacg atccggtacg cgtgattttc ttttcacatt aatctggtca
     2701 ataaccttga ataattgagg gatgacctca tttaatctcc agtagcaact ttgatccgtt
     2761 atgggaggag ttatgcgtct ggatcgtctt actaataaat tccagcttgc tcttgccgat
     2821 gcccaatcac ttgcactcgg gcacgacaac caattcatcg aaccacttca tttaatgagc
     2881 gccctgctga atcaggaagg gggttcggtt agtcctttat taacatccgc tggcataaat
     2941 gctggccagt tgcgcacaga tatcaatcag gcattaaatc gtttaccgca ggttgaaggt
     3001 actggtggtg atgtccagcc atcacaggat ctggtgcgcg ttcttaatct ttgcgacaag
     3061 ctggcgcaaa aacgtggtga taactttatc tcgtcagaac tgttcgttct ggcggcactt
     3121 gagtctcgcg gcacgctggc cgacatcctg aaagcaacag gggcgaccac cgccaacatt
     3181 actcaagcga ttgaacaaat gcgtggaggt gaaagcgtga acgatcaagg tgctgaagac
     3241 caacgtcagg ctttgaaaaa atataccatc gaccttaccg aacgagccga acagggcaaa
     3301 ctcgatccgg tgattggtcg tgatgaagaa attcgccgta ccattcaggt gctgcaacgt
     3361 cgtactaaaa ataacccggt actgattggt gaacccggcg tcggtaaaac tgccatcgtt
     3421 gaaggtctgg cgcagcgtat tatcaacggc gaagtgccgg aaggattgaa aggccgccgc
     3481 gtactggcgc tggatatggg cgcgctggtg gctggggcga aatatcgcgg tgagtttgaa
     3541 gaacgtttaa aaggcgtgct taacgatctt gccaaacagg aaggcaacgt catcctgttt
     3601 atcgacgaat tacataccat ggtcggcgcg ggtaaagccg atggcgcaat ggacgccgga
     3661 aacatgctga aaccggcgct ggcgcgtggt gaattgcact gcgtaggtgc cacgacgctt
     3721 gacgaatatc gccagtacat cgaaaaagat gctgcgctgg aacgtcgttt ccagaaagtg
     3781 tttgttgccg agccttctgt tgaagatacc attgcgattc tgcgtggcct gaaagaacgt
     3841 tacgaattgc accaccatgt gcaaattact gacccggcaa ttgttgcagc agcgacgttg
     3901 tctcatcgct acattgctga ccgtcagctg ccggataaag ccatcgacct gatcgatgaa
     3961 gcagcatcca gcattcgtat gcagattgac tcaaaaccag aagaactcga ccgactcgat
     4021 cgtcgtatca tccagctcaa actggaacaa caggcgttaa tgaaagagtc tgatgaagcc
     4081 agtaaaaaac gcctggatat gctcaacgaa gaactgagcg acaaagaacg tcagtactcc
     4141 gagttagaag aagagtggaa agcagagaag gcatcgcttt ctggtacgca gaccattaaa
     4201 gcggaactgg aacaggcgaa aatcgctatt gaacaggctc gccgtgtggg ggacctggcg
     4261 cggatgtctg aactgcaata cggcaaaatc ccggaactgg aaaagcaact ggaagccgca
     4321 acgcagctcg aaggcaaaac tatgcgtctg ttgcgtaata aagtgaccga tgccgaaatt
     4381 gctgaagtgc tggcgcgttg gacggggatt ccggtttctc gcatgatgga aagcgagcgc
     4441 gaaaaactgc tgcgtatgga gcaagaactg caccatcgcg tcattggtca gaacgaagcg
     4501 gttgatgcgg tatctaacgc tattcgccgt agccgtgcgg ggttggcgga tccaaatcgc
     4561 ccgattggtt cattcctgtt cctcggccca acgggtgtgg ggaaaactga gctttgtaag
     4621 gcgctggcga actttatgtt tgatagcgac gaggcgatgg tccgtatcga tatgtccgaa
     4681 tttatggaga aacactcggt gtctcgtctg gttggtgcgc ctccgggata tgtcggttat
     4741 gaagaaggtg gctacctgac cgaagctgtg cgtcgtcgtc cgtattccgt catcctgctg
     4801 gatgaagtgg aaaaagcgca tccggatgtc ttcaatattc tgttgcaggt actggatgat
     4861 gggcgtctga ctgacgggca ggggagaacg gtcgacttcc gtaatacggt tgtcattatg
     4921 acctctaacc tcggttccga tctgattcag gaacgcttcg gtgaactgga ttatgcgcac
     4981 atgaaagagc tggtgctcgg tgtggttagc cataacttcc gtccggaatt cattaaccgt
     5041 attgatgaag tggtggtctt ccatccgctg ggtgaacagc acattgcctc gattgcgcag
     5101 attcagttga aacgtctgta caaacgtctg gaagaacgtg gttatgaaat ccacatttct
     5161 gacgaggcgc tgaaactgct gagcgagaac ggttacgatc cagtctatgg tgcacgtcct
     5221 ttgaaacgtg ctatccagca gcagatcgaa aacccgctgg cacagcaaat actgtctggt
     5281 gaattggttc cgggtaaagt gattcgcctg gaagttaatg aagaccggat tgtcgctgtc
     5341 cagtaatgat aaaacgagcc cttc
//
LOCUS       DAJTQQ010000179         5350 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000179 DAJTQQ010000000
VERSION     DAJTQQ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.181"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(75..557)
                     /locus_tag="OO309_003967"
     CDS             complement(75..557)
                     /locus_tag="OO309_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848908.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVNVNGESGVTYENCKLNMKDINDD
                     "
     gene            complement(625..2574)
                     /locus_tag="OO309_003968"
     CDS             complement(625..2574)
                     /locus_tag="OO309_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5848909.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2659..2994)
                     /locus_tag="OO309_003969"
     CDS             complement(2659..2994)
                     /locus_tag="OO309_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR5848910.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2994..3350)
                     /locus_tag="OO309_003970"
     CDS             complement(2994..3350)
                     /locus_tag="OO309_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR5848911.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3350..4846)
                     /locus_tag="OO309_003971"
     CDS             complement(3350..4846)
                     /locus_tag="OO309_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR5848912.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVEKYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4843..5004)
                     /locus_tag="OO309_003972"
     CDS             complement(4843..5004)
                     /locus_tag="OO309_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR5848913.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(5014..>5350)
                     /locus_tag="OO309_003973"
     CDS             complement(5014..>5350)
                     /locus_tag="OO309_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848914.1"
                     /translation="RDEKGQWAAYDAVHDVRRELWKALLGWMPDPQGGEIVYVGGTLL
                     DLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGPDGDIEH
                     HLEMRFPQN"
BASE COUNT         1275 a         1561 c         1150 g         1364 t
ORIGIN      
        1 aaagagaagg taaagaaggt aagtccatac tgtctccact ataaaaaaac cgccaatttc
       61 tggcggctta tatattaatc atcattaata tctttcatat tcaatttgca attttcgtag
      121 gtcactcccg actcgccatt aacattaact ccagctctga tattcccgtt ttcttttaca
      181 tctacaaaaa actggcctct tggcatcttg aacgcaaatg taaaaccaac agtatctgaa
      241 tcaaaatcat catctttata tattgggatc aaaaatgtca tcgtcgctgg tgatgcattg
      301 tttgcaaaat caaatacacc tatacttttg ccgaattcat aagaagcttt tctcaatata
      361 atcgcaccgc tggcagaagg ggatgggcac gatatcctca gttgtctgaa tccatctgta
      421 tccaagtcaa cgtcattatc tgcagatgtt ttaacgttac taataaatcg tgaaacaatc
      481 tcctcatttg ttagtatttt cgcctgagca cagcaagagg ttaatattgc aaccgtcatt
      541 aaaaatggct ttatcacaaa acaaactcca ttgttagcat gttacgcgat aattttgcct
      601 catctttcac cacaaaaaaa gaactcacct tttaaatgga gaatacccaa catcatgggt
      661 gattttcata aagggatcgc ctgttttcgg caaatcgata acgcgcattc caggcggcgc
      721 gttgtcaaac gtcaccttaa gttcgctgcg ggttgccggt gaagatgcaa ggcttaacag
      781 ctgattcctt tccagtgata cattcggggt gtagcgttta tcgggagata aatacccatc
      841 accacgtggc atctgccatc cggtcatgtc atagacaaaa tcatggaact gtttgcccca
      901 ctcgtcgaac ttatcattca gaccaaatcc actgttcagt gcatcagcca caaaattttt
      961 aacaagccag ggatgttctt tttcaaactc ctgcgccatc atgcccagtt caagcaatcc
     1021 accaatcaga ctgatctttc cgaggccctt taatttcagc cctgtttttc ctgccgccgt
     1081 tgtccattga ttctgggcga ctgttgccgc acgaatactt gccaccgcct tcacaccaat
     1141 atacagggca gaaaccgttg ctattgtttt tactgcctgt tcccatcccc ccattgctct
     1201 gacaacctga tcaacctcct gccagacttt tttcacaacc ggaccgaccg tttcccagtt
     1261 atcaattatg agatatgcac caccgacgag aagcgcaatt aatccctttg ccggcgtcat
     1321 attcatcacg ctacccatga tttttgttat gcgtgtcaat gtgccaatcg ccacgcccat
     1381 cgtcagtaat gccgcaccag ttttcgcgat ggtttttacg acctcaggat tttctctgac
     1441 aaacgtgcgc acttcttcca gaaaaggctt catctctttt atgccttcat tgagtgaagg
     1501 caggaaggtt tcccccagcg tggaagaaat cgcattgatc tggttctgca gcagcaacaa
     1561 ctgattttct gtcgtcgcgg cacgggcggc atattctttc tgcattgagc cgccatactg
     1621 ctgcgcatcc gccacacgat taaagttggt tcgcaacaaa tccagattcg tgagcagcgg
     1681 cgcaatggcc cccagggatt ccttcccgaa cagcgctttc agcacagcag cctgtttttc
     1741 tttcggcact tttgccagag aatccagtac gtgcagcatg gccccgcggg cgtctttctg
     1801 catatccgcc gccagttttt tcgggctaat gcgcaacgag cggagcactt ttttctgcga
     1861 tttggtggca gaatccctcg ctgtcagcga caacataaaa ttctttatcc ccgtcgcagc
     1921 aatttctgac tccaccccca ttccggcaat ggttgccccc attgcggcaa tctctccgga
     1981 ggccacaccc gcaacactgc ctaaagggcc aatacgggtc acaacatcag aaatcttttt
     2041 cgcacttgca ggaccggtat tgccaagata gttaatctta tccgcaagcc ctgccacctc
     2101 tccctgtgtc agtttaaagg cagtgcgcca ctgcgccatc atctgaccgg attcttctgc
     2161 cgtggtgtca aaggccacgc ccatcttcac ggcatcgtca gtaaactgca tcagctcgtc
     2221 acgggcgata ccagcctgac cacccgccgc cacaatttcg gcgatacctt ccgccgacat
     2281 gggcagttct gttgacaggt cgcgcacctg ctccgtcatt gccttaaacg cttccggcgt
     2341 atccagacca tccaccactt tccggacatc cgccattttt gattcaaggg caatggcaga
     2401 tttgaccggg agcgccagcg cccccagtac tgcggttccg gcacccgctg cgcccagtga
     2461 caggctggca aactcctttt taaaaccttt cagctggcgc tgcattccct tcagcggtgc
     2521 cgatacctta tccacggcag tgatgatcgc cttcagctga aaactgtcag ccatgcttca
     2581 tctcctcgtt aatgcggacg gcctcggctt ccagttctgt aaactgcgaa acagccaccc
     2641 gtcgcagctc cagtggattc agtttccaga accaggcaac attgtagagt cgtttccgga
     2701 ggtgcttccc gtctccgact gggtaaaaaa acgcaggatc tgcatgctgg tcttaaaaat
     2761 atccagtttt gccatctgcg ccgccgatga acacgggatc cccgccagca acgggatata
     2821 tttcagtgcc acctggctgt ccagtttaat actgccttca ccggaaataa tgaaaggaaa
     2881 acccagcgcc tcgatttcgt catacgtggg ctcgcgcaac tccagcacat gcagcatttc
     2941 gttatgcgct gtcaccggtt tttttaaaac tatttctgta acgttcattg ccattactga
     3001 taccctccct cttcgccgtg gaattcaaga tctgccgtgc cttcttcggc attatgatta
     3061 gcctctccgt gcagccatgc cgccgaaagc acatacacca tgccgtttgc cagctcagcg
     3121 gtaatcgtca tctggtctga ggtggtaatt ttgtcgaccg gaaagttttt gggcacttta
     3181 aacgtgccct taacgtaagg tgaccgccag gtctccttgt aatccacatc ccctgccatg
     3241 ccgacgatgt catcccggac attggtgttc atcggcactt caatgccacc tgtcagcgat
     3301 aactgctgac catccacttt aaaaaaacag gttccggcga tctttgccat tatgctgact
     3361 cctctgaata ctgaagacgg aactggttaa ccaccgcaaa gacacgtaac tgattaacat
     3421 aatccggcgg gaacagcgta ttcagtcggt tcgggttatc tgcatcacgc tcaactatca
     3481 gatactgttt aaacagatcg tatttttcca caataccggc acgctccatc tgacgatatg
     3541 tcgccagaag ttcccctttg ataacggcag gagtgacaat cgcctgcccc ggaccaaaac
     3601 gggtaccatc atttgccagc ttgtgacgtc cgtacttgct tgtgatgacc gatttcagtt
     3661 tgcgaagaac atatgcactg gtatgcagag tttcactgtc cagatagctg ttgtctgcca
     3721 cgccatacgc attctttttg taggtggtta cggaacgctg gatctgcaac gtgccgcctt
     3781 ccacataagc cgtcgccacg ccgtgagata aaagggtctg ctgctctgtc atgatgaatc
     3841 gcttaccttt cggtgccgga agcatcccca ccagctcccc cgtctgtgtc ggacgggcag
     3901 gatcattccg gataaatacc gcttcacggg caaggcgact ggcaaccagt tcatcgacag
     3961 gcgactgggt ttctttttcg taaccggcaa gcgtgatatg ttgctgatta tgcatatctc
     4021 cggcaccaac cagctctgac agcgttccca gttttgcggt atagacatgc ccgtataact
     4081 ggcgcgcata actccagcga ccgctgctgt cattcatttc ggtcatcatc atattgatgg
     4141 aggcggcatc gttgaacggc agaccgataa agtcgaatgc ctcatcgccc atagcagcaa
     4201 cagcggcggt aagatcaggc gctccgctac cggcagcacc gacttccgtc accacccgaa
     4261 gccctgctgg caaaatctca ccaccaccag aaccataata attcagacag accggtaact
     4321 cattaccata cagcccctta tgacgggcag tcagcgtcac cacgcctgca tcagatgacg
     4381 ccgtaaacgg cagggtgata accccgttta ccgcttcctt aatcgcggtg gcaaccgcag
     4441 tggcatcatc gccattcacc acaggcacct gtacacggga gcgcccgaca tacagactca
     4501 gggtgccgct ttcctctgct tctccggcaa ccgtcaccct gaccgtcgcc gccgcccctc
     4561 tggcttccgg tactgcaata acatacagtt caccgaaagg atctgtctga cggtagactt
     4621 ccaccatacg cgccagctgg ctccccgccc cacaaatctg gcgggcataa tctgccgatg
     4681 gcatcagcac caggctgtta acctcaatgg ctgcatcgtt gctggcatgc ccgatcagta
     4741 atgcaggcgc gctggtcacc gctgtatttg ccgcagaatt gttcatctcg gcgtagaaca
     4801 acggcaccag cgtattcgac ggaacagtat taaaacttat cgtcatgatt ttttagcctt
     4861 atcctgttta acacgtacca catcacccgc cgctatacgg cggaaccagt agctgctttc
     4921 ttccacattt cgcccttcgg aaggcaaaag gtctccacgg gcagggtcag gaaccgaccg
     4981 ccctttcagg ggtttcacaa acatgaggga ctcttaattc tgagggaaac gcatttccag
     5041 atggtgctca atgtcaccat ccgggccagt accaggatcg atgtaatcca catcaatact
     5101 cagcagggat aaatccggca gggcattcac gtcctctgcc tgtcgcgtgt cttcttccgt
     5161 gatttcatac ttcgccgtaa aatcaaactg gtaatacagt tcgtaacggt tcagatccag
     5221 cagggtgcca ccgacataaa caatctcgcc cccttgcggg tccggcatcc atccaagcag
     5281 ggctttccag agttctctcc ggacatcatg aacggcgtca taggctgccc actgcccttt
     5341 ttcatcacgc
//
LOCUS       DAJTQQ010000180         5326 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000180 DAJTQQ010000000
VERSION     DAJTQQ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.182"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            245..429
                     /locus_tag="OO309_003974"
                     /pseudo
     CDS             245..429
                     /locus_tag="OO309_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            572..793
                     /locus_tag="OO309_003975"
                     /pseudo
     CDS             572..793
                     /locus_tag="OO309_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(814..876)
                     /locus_tag="OO309_003976"
                     /pseudo
     CDS             complement(814..876)
                     /locus_tag="OO309_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1326..2738
                     /gene="ospC1"
                     /locus_tag="OO309_003977"
     CDS             1326..2738
                     /gene="ospC1"
                     /locus_tag="OO309_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR5848915.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKSFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2842..2991)
                     /locus_tag="OO309_003978"
                     /pseudo
     CDS             complement(2842..2991)
                     /locus_tag="OO309_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            3067..4764
                     /gene="ospD3"
                     /locus_tag="OO309_003979"
     CDS             3067..4764
                     /gene="ospD3"
                     /locus_tag="OO309_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR5848916.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
     gene            4881..5000
                     /locus_tag="OO309_003980"
                     /pseudo
     CDS             4881..5000
                     /locus_tag="OO309_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
     gene            5167..>5326
                     /locus_tag="OO309_003981"
     CDS             5167..>5326
                     /locus_tag="OO309_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848917.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFH"
BASE COUNT         1792 a         1011 c         1027 g         1496 t
ORIGIN      
        1 tctccaggat tcccggggcg gttcaatcaa agcgcagaac ttctgcgtct tcttcgtcaa
       61 aaaaatcatc ttcgtgaagc ttcacgacat agcggggaag tttgcttctt tgagaggcgg
      121 gtttacgttt acgggattta gctgaacggg ccatataacc acctgaaaga caatgacatt
      181 gcctgttttt ataacggtaa ttgcagacca tgacaaaccg cagccgtcag gctgcctact
      241 cgagtcacaa ggctctttgt gtccgatatg ccggcagcgc attacccatg aaactggctg
      301 gaacgtacac cataaggtga agaaagttat gggggcggtg aagaattatc taaccttgtc
      361 ttgttacatc caaattgcca tcgacagtta cacagttgtg aagccggctc tctcaaaaag
      421 ggcttataaa ggcttgagcg gtgtaccggg aaaccggtat gcaccgttct taggggaggg
      481 cagtccagca atgaactgtc cttacccgac aaacatccaa aacgaacgga acgtgctgga
      541 atccgcatat aacccgttgt aaagtgagga aatggacacc tcacttgctc atgagaacgc
      601 ccgcctgcgg gcactgttgc agacgcaaca ggacactatc caccagatgg ctgaatacaa
      661 ccgcctgctc tcacagcgga tggcggctta tgcttccgaa atcaaccgac tgaaggcgct
      721 ggttgcgaaa ctgcagcgta tgcagttcgg taaaagctca gaaaaacttc gcgcaaaaac
      781 cgaacggcag attccatttt cacgggcgat ctacgccaca caggctctgg gttgcagtga
      841 ctgctccatt aaggcgattt tgaactcata agggaagtaa caaaaaaact gcaaaagtac
      901 aataattata tatggccaaa ctaacctcta ttaaaaaaca tcagtttttc acacttagtt
      961 gagcttcatg aaaaaatcat cgaggatttc attgcagaat acacattcca ctaactggaa
     1021 gcactccata agaggaatca gagaaagtat cgttttttta tagtaaaatt cattgctgtt
     1081 caataaaatt gatatagtta ttaaattatg aaaataattt cacaaaaaag attaacttga
     1141 gttcttaagc atcggatttt agatattttg aaatatctgg catcacataa catcaaaagt
     1201 atataatact cttatctaat tacatgcagt atattgtata tatactcaaa aattgtaatt
     1261 cataccctgc tatatcttgt agggaaagat attaattaaa actgttttca tataaggttc
     1321 attttatgaa tatatcagaa acactgaact cagcaaatac ccaatgcaat atagattcta
     1381 tggataacag attacataca ttgtttccaa aagtgacatc agtgcgaaac gctgcacaac
     1441 aaactatgcc agatgaaaaa aatttaaaag atagtgcaaa tattattaaa agtttcttta
     1501 ggaaaactat agcagcacag agttatagta gaatgttctc tcaaggctct aactttaaat
     1561 ctttaaatat agcaattgat gcaccatcag acgctaaagc ctcatttaag gctattgagc
     1621 accttgacag attatcgaag cattatatat ctgaaataag ggaaaaactt catcctcttt
     1681 ctgcagagga actcaatttg ctttcgctaa ttattaattc tgatttaatc ttcagacatc
     1741 aaagtaattc tgatttgtct gataaaattt taaacattaa gtcattcaat aaaattcagt
     1801 ctgaaggaat atgcacaaaa cgaaacacat acgctgatga tataaaaaaa atagctaatc
     1861 atgactttgt gttttttggc gttgaaatct ctaaccatca gaaaaaacac cccctgaata
     1921 caaaacatca cactgttgat tttggtgcaa atgcgtatat cattgatcat gactctccat
     1981 atggatatat gacattaacc gatcactttg ataatgctat tccacctgtt ttttaccatg
     2041 agcaccaatc atttttagat aaattttcag aggttaataa agaagttagt cgatacgtac
     2101 atggaagtaa aggaattata gatgtaccaa tattcaatac taaagatatg aagttagggc
     2161 tcggattata cctgattgac tttattagaa aaagtgaaga ccaaagcttc aaggagtttt
     2221 gctatggaaa aaatcttgcc cctgtggatc tggatagaat cataaacttt gtttttcagc
     2281 cagagtacca tatacctagg atggtaagta cagaaaactt caaaaaagtt aagattagag
     2341 aaatatcctt agaggaggct gttacagcat ctaattacga agaaattaac aagcaggtca
     2401 ctaacaaaaa aattgctctc caggctcttt ttctttcgat tactaatcaa aaagaggatg
     2461 tcgccttata tatattatct aattttgaga taactagaca agatgttatt tccataaagc
     2521 atgagttgta tgatattgag tatctactta gcgctcataa ttcaagctgt aaagtacttg
     2581 agtattttat caataaggga ttggttgatg taaacacaaa gttcaaaaaa actaatagtg
     2641 gggattgtat gttggataac gcaataaaat atgagaatgc agaaatgata aaactattat
     2701 tgaaatatgg tgcaacatct gacaataaat atatttaatc aaaattgaat atcgtttagc
     2761 aaaaggcagt atttgcacaa aatatcgata tattgtttat tgtcagtatg gctcaatgtg
     2821 ataatagttg gaaagtttga tgggtttcgc cccgttgtag cggtagtcga actggaaggt
     2881 cttcaggcac tgcttacagc gatagagcag cccccctgaa ctggaatggc cgttccggat
     2941 acccccctga gtttcagagt aacggtgaca aaccacatca atctttgcca tcaatcatcc
     3001 aaagggcaaa gagtacaaca acactaagtc tgcgtcacaa cccatcaatg aaaggaatat
     3061 atacatatgc catcagtaaa tttaatccca tcaaggaaaa tatgtttgca aaatatgata
     3121 aataaagaca acgtctctgt tgagacaatc cagtctctat tgcactcaaa acaattgcca
     3181 tatttttctg acaagaggag ttttttatta aatctaaatt gccaagttac cgatcactct
     3241 ggaagactta ttgtctgtcg acatttagct tcctactgga tagcacagtt taacaaaagt
     3301 agtggtcacg tggattatca tcactttgct tttccggatg aaattaaaaa ttatgtttca
     3361 gtgagtgaag aagaaaaggc tattaatgtg cctgctatta tttattttgt tgaaaacggt
     3421 tcatggggag atattatttt ttatattttc aatgaaatga tttttcattc cgaaaaaagc
     3481 agagcactag aaataagtac atcaaatcac aatatggcat taggcttgaa gattaaagaa
     3541 actaaaaatg ggggggattt tgtcattcag ctttatgatc ccaaccatac agcaactcat
     3601 ttacgagcag agtttaacaa atttaactta gctaaaataa aaaaactgac tgtagataat
     3661 tttcttgatg aaaaacatca gaaatgttat ggtcttatat ccgacggtat gtctatattt
     3721 gtggacagac atactccaac aagcatgtcc tccataatca gatggcctga taatttactt
     3781 caccccaaag ttatttatca cgcgatgcgt atgggattga ctgagctaat ccaaaaagta
     3841 acaagagtcg tacaactatc tgacctttca gacaatacgt tagaattact tttggcagcc
     3901 aaaaatgacg atggtttgtc aggattgctt ttagctttac aaaatgggca ttcagataca
     3961 atcttagcat acggagaact cttggaaact tctggactta accttgataa aacggtagaa
     4021 ctactaactg cggaaggaat gggaggacga atatcgggtt tatcccaagc acttcaaaat
     4081 gggcatgcag aaactatcaa aacatacgga aggcttctca agaagagagc aataaatatc
     4141 gaatacaata agctgaaaaa tttgctgacc gcttattatt atgatgaagt acacagacag
     4201 atacccggac taatgtttgc tcttcaaaat ggacatgcag atgctatacg cgcatacggt
     4261 gagctcattc ttagcccccc tctcctcaac tcagaggata ttgtaaattt gctggcctca
     4321 aggagatatg acaatgttcc cggacttctg ttagcattga ataatggaca ggctgatgca
     4381 atcttagctt atggtgatat cttgaatgag gcaaaactta acttggataa aaaagcagag
     4441 ctgttagaag cgaaagattc taatggttta tctggattgt ttgtagcctt gcataatgga
     4501 tgtgtagaaa caattattgc ttatgggaaa atacttcaca ctgcagacct tactccacat
     4561 caggcatcaa aattactggc agcagaaggc ccaaatgggg tatctggatt aattatagct
     4621 tttcaaaata ggaattttga ggcaataaaa acttatatgg aaataataaa aaatgaaaat
     4681 attacacctg aagaaatagc agaacacttg gacaaaaaaa atggaagtga ttttctagaa
     4741 attatgaaga atataaaaag ctgaatatta tctcgtggtg atacactgaa tttgaggtaa
     4801 ccggctcatt taaaccgtct ggtctgttac ctccggttcc acaaaaataa tgtccatcat
     4861 tttgaagtgg acactatcgt atggaatacc ggacctggat cactgaagct ttacgccttc
     4921 atttcgaaga acatttacca cgggttgtgg ccggacgtcg cctgggcgta ccaaaatcaa
     4981 ctgtctgtgg tatgttcgtg tgaaccgccc ccggtatccc gctcatgctg gcgaacccag
     5041 acacgcagtg aaccgccccg ggtttcctgg agagtgtttt atctgtgaac cacggcagcc
     5101 tggcggctgc aaatcggggt gtggcagaat atgagctttc agagtaacca tccagaatga
     5161 gttcccatgt cacacaacct ggagcaccag aaagtccaca ccagaatggt gaaagaggtt
     5221 ctgaaagccg tcgccagagc caataatcac ccttataagt ccgtatttgc agactttatc
     5281 accggtcatc cctcctgcac ggtctgtttc tgggaaacct tccaca
//
LOCUS       DAJTQQ010000181         5131 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000181 DAJTQQ010000000
VERSION     DAJTQQ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.183"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            180..437
                     /locus_tag="OO309_003982"
                     /pseudo
     CDS             180..437
                     /locus_tag="OO309_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(487..1437)
                     /gene="uspE"
                     /locus_tag="OO309_003983"
     CDS             complement(487..1437)
                     /gene="uspE"
                     /locus_tag="OO309_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR5848918.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1589..2341)
                     /gene="fnr"
                     /locus_tag="OO309_003984"
     CDS             complement(1589..2341)
                     /gene="fnr"
                     /locus_tag="OO309_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR5848919.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2536..3051)
                     /gene="ogt"
                     /locus_tag="OO309_003985"
     CDS             complement(2536..3051)
                     /gene="ogt"
                     /locus_tag="OO309_003985"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR5848920.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3062..3205)
                     /locus_tag="OO309_003986"
                     /pseudo
     CDS             complement(3062..3205)
                     /locus_tag="OO309_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3595..4410)
                     /locus_tag="OO309_003987"
     CDS             complement(3595..4410)
                     /locus_tag="OO309_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5848921.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4507..>5131
                     /locus_tag="OO309_003988"
     CDS             4507..>5131
                     /locus_tag="OO309_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5848922.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDL"
BASE COUNT         1369 a         1170 c         1225 g         1367 t
ORIGIN      
        1 cgcgacaatt cattcagaac atcaatagtc agggaaggaa gtagcaacat tagctaacta
       61 atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag acaacggtga
      121 aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag gattacatca
      181 tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt ttgttggcgg
      241 ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt atagaacagt
      301 acaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca tcgatgattt
      361 ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt tatttccttg
      421 gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc agttattgaa
      481 gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact ggtcaggttt
      541 aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt tgccgaggaa
      601 tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg cctgtaaatg
      661 ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg tcatgttttc
      721 attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc cacgaatggc
      781 atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa ttggcgttac
      841 cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca gttcgatcgt
      901 ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg cgagattcac
      961 cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc acaccgggct
     1021 tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt ccagacggtc
     1081 atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt cctgaatgat
     1141 ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc cagcattgag
     1201 ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc cctgacgcat
     1261 agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt catagatcgg
     1321 caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc gccgcaatgc
     1381 tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca tagccataca
     1441 gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc catatctggt
     1501 gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt aatgatatga
     1561 cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatgg ccagcaagct
     1621 gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc agcataccgc
     1681 ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc agatagttac
     1741 caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg cgttgggcaa
     1801 aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt ttcttcgaca
     1861 acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc atcatctgct
     1921 gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga atttcacata
     1981 ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg atggcgtcaa
     2041 atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct tgctcagtga
     2101 ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt tcatcaccag
     2161 ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata ttatcaagct
     2221 gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg ctgcaatcct
     2281 ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt tccgggatca
     2341 taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta gggagcaagt
     2401 aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata tatgtctatc
     2461 tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa caagctggca
     2521 caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc actcttttcg
     2581 ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc gatggcaagg
     2641 tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg ccgcgccagg
     2701 gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg ggatagtgcg
     2761 tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg gaagcgtatc
     2821 aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac cacctgggtt
     2881 ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca gctgcaccat
     2941 gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat cgcaaatcac
     3001 ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca tccgttctct
     3061 cttaagacaa acgtgggtaa ataccctgac ctatcggcag accgacaaga taccacgcca
     3121 gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact aacgaatagt
     3181 aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga ttttttaatt
     3241 tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga taattccagt
     3301 cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata gccgccagac
     3361 accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt tacagagcta
     3421 aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt acacacagca
     3481 gtgcatcctc gtcttaaatc cggcctgggg tccaggtgac cggtgacagt gttataacgg
     3541 ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata taagttaaat
     3601 catttgtctt gtgattgcat aattatgcat cgttaatcga cgagtaaatt taccgggcga
     3661 gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat agcccagacg
     3721 atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag aaataacgtg
     3781 catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata agcgcatgag
     3841 tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca gatacccttg
     3901 gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc aagggagttc
     3961 aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga gggctgaaaa
     4021 acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa ttgcaattag
     4081 taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca gcgtttcact
     4141 ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa caaacttatg
     4201 cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga cgctttctcc
     4261 agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga gatgtttatg
     4321 ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt gatgccctgg
     4381 tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca gaaatcatca
     4441 aataagccat gttaaatttt aaatatgacg tatgcaagtt tccggatata tacggtgtaa
     4501 agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg tattcttttg
     4561 actggcgttg tcttttttat ctggagggta agcaaaggaa gatttataca gacgacggat
     4621 gatgcttaca ttggtggtaa tatcaccacg gtggcaagta aagtttctgg ttatatttct
     4681 gctatagagg tgcgcgataa tcagagcgta aaaaaaggcg atattatcct gcgtctcgat
     4741 gatcgggatt atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag tagcaaagcc
     4801 aatctcgaag gcattcaagc cactattacg atgcagcagt cgataattca aagtgccagt
     4861 gaaacatggc aggcagtaaa acatgaggag caaaagcgcc tgcgagatac tgaacgttat
     4921 gaaaaactcg cacaatcagc ggcaatatcg caacaaatta ttgataacgc caggtttgac
     4981 taccagcagg tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt ggagaagcaa
     5041 cggctggcag ttttatctgc tcaggaagaa aacgtacgtg cgagtatcga agaagttcag
     5101 gccgctttga ctcaggcact actggatctg g
//
LOCUS       DAJTQQ010000182         5019 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000182 DAJTQQ010000000
VERSION     DAJTQQ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.185"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..165
                     /locus_tag="OO309_003989"
     CDS             <1..165
                     /locus_tag="OO309_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5848923.1"
                     /translation="HRKSWKNRTEVELATLTWVDWYNNRRLLERLGHIPPAEAEKAYY
                     ASIGNDDLAA"
     gene            330..4448
                     /gene="pic"
                     /locus_tag="OO309_003990"
     CDS             330..4448
                     /gene="pic"
                     /locus_tag="OO309_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HCR5848924.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4599..4841)
                     /locus_tag="OO309_003991"
                     /pseudo
     CDS             complement(4599..4841)
                     /locus_tag="OO309_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(4880..>5019)
                     /locus_tag="OO309_003992"
     CDS             complement(4880..>5019)
                     /locus_tag="OO309_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5848925.1"
                     /translation="SLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSELLPWRIALPA
                     E"
BASE COUNT         1338 a         1147 c         1340 g         1194 t
ORIGIN      
        1 caccgtaaga gctggaaaaa ccggacagaa gtggagctgg ccacactcac gtgggtggac
       61 tggtataaca atcgacgatt gctggaaagg ctgggccata tcccaccggc agaagcagaa
      121 aaagcttatt atgcttccat cggaaatgat gatctggcag cctgagttca cagataaaac
      181 actctccagg aaacccgggg cggttcagtt cacaaaaaca cattaatgca gtaactatat
      241 tttcctttct ggtgataacg tcgggttatc attagcttct tcagctattt tacttttata
      301 tcccttgtaa acatcatgga gaatccatag tgaataaagt ttattctctt aaatattgcc
      361 ccgtcaccgg ggggcttatt gctgtctctg aacttgcccg cagggtaata aaaaagacat
      421 gccgaagatt aacgcatatt cttctggctg gcattccagc aatctgtctg tgttactctc
      481 agatatccca ggcgggtatt gtccgttccg atattgccta tcagatttat cgtgatttcg
      541 ccgaaaacaa agggcttttt gtacctggtg ccaatgatat tccggtatat gataaggacg
      601 gaaaacttgt gggaagactg ggtaaagccc caatggccga tttcagcagt gtgagctcaa
      661 atggcgttgc tacgcttgta tcgcctcagt atatcgtcag cgtaaagcat aacggaggat
      721 atcggagtgt gagctttggt aatgggaaaa atacatattc ccttgttgac cgtaataacc
      781 acccttctat tgacttccat gctccacgtc tgaataaact ggttacagaa gttattccct
      841 cagcggtaac atcagaagga accaaagcca atgcttataa atacactgaa cgttacaccg
      901 ctttttatcg ggtgggtagt ggtacgcagt acactaagga caaggacgga aatttagtta
      961 aggttgccgg tggatatgct tttaaaacag gaggaaccac aggagttcct ctgatatctg
     1021 atgcaacaat agtctctaat cccgggcaaa cttataatcc tgtaaacggc cctttacctg
     1081 actatggagc ccctggggat agtggttctc ctttgtttgc ttatgataaa caacaaaaaa
     1141 aatgggttat tgttgctgta ttaagagcat atgcaggtat taatggtgct acgaactggt
     1201 ggaatgtcat accaacagat tatctgaacc aggttatgca ggacgatttc gatgcccccg
     1261 tagactttgt ttccggactg ggccccctga actggacata cgacaaaaca tcaggcacag
     1321 gtaccctgag ccagggcagt aaaaactgga ccatgcacgg gcagaaagac aatgacctca
     1381 atgccggtaa aaatctggta ttcagcgggc agaatggtgc aattatcctg aaagacagtg
     1441 tgactcaggg tgccggttat ctcgaattta aagacagtta caccgtatct gctgaatccg
     1501 gaaaaacatg gacgggtgcc ggcattatta ctgacaaggg gacgaatgta acctggaagg
     1561 tcaacggcgt tgccggtgac aacttgcata agctggggga aggaaccctg accataaacg
     1621 gaacaggtgt aaacccggga ggactgaaaa cgggagacgg tatcgttgta cttaaccagc
     1681 aggcagacac tgcaggtaat atccaggcct tcagttcagt gaacctcgcc agcggacgtc
     1741 cgaccgtggt gctcggggat gcccgtcagg tcaatccgga taacatttca tggggatacc
     1801 ggggaggtaa gcttgacctt aatggtaatg ccgttacctt cacccgactg caggctgctg
     1861 attacggggc ggtgattaca aataatgcac agcaaaaatc ccagctttta ctggatctta
     1921 aggctcagga tacaaatgtc agtgaaccga cgattggaaa tatatccccc tttggtggta
     1981 ccggcactcc aggaaacctg tacagcatga tactcaacag ccagacccgc ttctatattc
     2041 tgaaatctgc cagctatggt aacactctgt gggggaacag cctgaatgat ccggctcagt
     2101 gggagtttgt tggcatgaac aaaaacaaag cagttcagac agtaaaagat aggatcctgg
     2161 ccgggcgggc aaaacaaccc gttatctttc atggtcagct gaccgggaat atggatgtcg
     2221 ccattccaca ggtgccgggg ggaagaaagg taatctttga tggtagcgtg aacctgccgg
     2281 aaggtaccct gagtcaggac agtggcaccc tgatattcca gggacatccg gttatccatg
     2341 cctccatcag tggcagtgca ccggtcagcc tgaaccagaa agactgggaa aaccgtcagt
     2401 ttacaatgaa aacactgtcg ctgaaagacg ctgacttcca tctttcacgt aacgcctcgc
     2461 tgaacagtga cattaagtcg gataacagcc atatcacact gggaagtgac agggcatttg
     2521 tggataaaaa tgacggaaca ggaaattatg tcattccgga ggaaggtacc tctgtcccgg
     2581 acaccgtgaa tgacaggagc cagtatgaag ggaatattac gctgaaccat aactcagccc
     2641 tggatatcgg cagcaggttc accgggggga ttgacgctta tgacagtgcc gtcagcatca
     2701 cctctccgga cgtcctgttg acagccccgg gtgcttttgc cggcagttca ctgacagtgc
     2761 atgatggcgg tcatcttaca gcactgaacg gtcttttcag cgacgggcat attcaggccg
     2821 gtaagaacgg caaaatcacc ctgagcggta caccggttaa agatacggct aatcagtatg
     2881 cccctgctgt atatctgacg gacggatatg acctgaccgg cgataacgca gcactggaaa
     2941 ttacccgtgg agcacatgct tccggtgata ttcatgcctc tgcggcatca acagttacca
     3001 tcgggtctga cacgccggca gaactggctt ctgcggaaac ggctgcatcg gcgtttgccg
     3061 gcagtcttct tgagggctat aacgcagcat tcaatggtgc cataaccggt ggcagggctg
     3121 atgtcagtat gcataatgca ctgtggactc tgggtgggga ctctgccatc cacagtctta
     3181 ccgtcagaaa cagccgtatt agttctgaag gagaccgtac attccgtacc ctgacggtga
     3241 ataaactgga tgcaacaggc agtgattttg ttttgcgtac ggacctgaaa aatgccgata
     3301 aaattaatgt gactgaaaaa gccactggtt cagataacag cctgaacgtc agctttatga
     3361 ataatcctgc tcagggacag gccctgaata ttcctctggt cacggcaccg gcgggaactt
     3421 cagcagagat gtttaaggcc ggcacccggg tgacaggttt cagtcgggtg accccaaccc
     3481 tgcatgttga taccagtggt ggcaatacga agtggatact ggatggtttt aaagcggagg
     3541 ctgataaagc cgctgccgcg aaggctgaca gtttcatgaa tgccgggtat aaaaacttca
     3601 tgacggaagt taacaatctg aacaaacgta tgggtgacct gcgtgacaca aacggtgatg
     3661 ccggtgcctg ggcgcgcatc atgagtggtg ccggttctgc agacggtggt tacagtgata
     3721 attacaccca tgttcaggtc ggctttgaca aaaaacatga actggacggt gtggacctgt
     3781 ttaccggtgt cacgatgacc tataccgaca gcagtgcaga cagccatgca ttcagcggaa
     3841 agacgaaatc ggtggggggc ggtctgtatg cttcagcatt gtttgagtcc ggtgcctata
     3901 tcgatttgat tggtaaatat attcaccatg acaatgatta cacaggtaac tttgctagcc
     3961 tgggaacgaa acactacaac acccattcct ggtatgccgg tgctgaaacg ggttaccgct
     4021 atcacctgac agaggacacg ttcattgagc cgcaggctga actggtttac ggcgccgtgt
     4081 ccgggaaaac attccgctgg aaagacggtg atatggacct gagcatgaag aacagggact
     4141 tcagtccgct ggttggaaga acaggggttg aactgggcaa gaccttcagt ggtaaggact
     4201 ggagtgtgac ggcccgtgcc ggaaccagct ggcagtttga cctgctgaat aatggagaga
     4261 ccgtactgcg tgatgcgtcc ggggagaaac ggataaaagg agagaaggac agccggatgc
     4321 tgtttaatgt tggtatgaat gcgcagataa aggacaatat gcgctttggt ctggagtttg
     4381 agaagtcagc ctttggtaaa tataacgtgg ataatgcggt aaacgcgaat ttccggtata
     4441 tgttctgatg tgggggtaaa gggctgccgg aaggcagcct ttctcagaga ctcttaaaat
     4501 ataaaactga aggtcttcgg taagggggag tgtattagac gtcaactagt tttgccgact
     4561 ggcgcgagcc tgtttaagat tctgtgtaaa tgcctttctc agaagtgacc gtccaggcgg
     4621 tcaccgaact cgataataaa gcggctcatt gccattcgcc agtccttcaa cggcatcgtc
     4681 catttctggg acgcagactg gattgccagc cacaccactt ttttcaccga gtcgtctgtc
     4741 gggaacactt tacgcttttt gatcgcatgg cggatcacgc tgtttagcga ctcgatggca
     4801 ttcgtcgtat agatcacttt gcggatgtcc gttggataag cggtaagcgt acagcgaggg
     4861 ccgtattgac ggggatgtgt tattcagctg gcagtgctat gcgccacgga agcagttcgc
     4921 tgacccggtt gaccggccag tctgctatga cgccaagcac atggcgaagg tagctttctg
     4981 gatccacgtc attcagtttg cacgtcccga tcaggctgt
//
LOCUS       DAJTQQ010000183         4927 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000183 DAJTQQ010000000
VERSION     DAJTQQ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.187"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..229
                     /locus_tag="OO309_003993"
     CDS             <1..229
                     /locus_tag="OO309_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR5848926.1"
                     /translation="VVMLAKSVTAQTHMAWSDADNQQVKLSMPELEELAAAMVQAQVD
                     RNDEIYRRQRELKEELNSLKDLNSVRNFIVE"
     gene            complement(430..1287)
                     /gene="sitD"
                     /locus_tag="OO309_003994"
     CDS             complement(430..1287)
                     /gene="sitD"
                     /locus_tag="OO309_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR5848927.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1284..2141)
                     /gene="sitC"
                     /locus_tag="OO309_003995"
     CDS             complement(1284..2141)
                     /gene="sitC"
                     /locus_tag="OO309_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR5848928.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2138..2965)
                     /gene="sitB"
                     /locus_tag="OO309_003996"
     CDS             complement(2138..2965)
                     /gene="sitB"
                     /locus_tag="OO309_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR5848929.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2965..3879)
                     /gene="sitA"
                     /locus_tag="OO309_003997"
     CDS             complement(2965..3879)
                     /gene="sitA"
                     /locus_tag="OO309_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR5848930.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4311..>4927)
                     /locus_tag="OO309_003998"
     CDS             complement(4311..>4927)
                     /locus_tag="OO309_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5848931.1"
                     /translation="NALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVDFEKGIVN
                     VRRNLTILDMFGPPKTNAGIRTVALLQPALEALKEQYKLTGHHRKSEITFYHREYGRT
                     EKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHTFACWLLT
                     AGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1308 a         1204 c         1177 g         1238 t
ORIGIN      
        1 ggtagtcatg ctggcaaaat ctgtcacagc acaaacacat atggcgtgga gcgatgccga
       61 taatcagcag gtgaaactgt cgatgccgga actggaagaa ctggcggcag caatggtgca
      121 ggcgcaggtc gatcgcaacg atgagattta tcgccgtcag cgggagctga aggaagagct
      181 gaatagtctg aaggatttga attcggttag gaattttatc gtggaataac agaagctgcg
      241 gcacgtcgta tgcaggaacg tgccgcggtt ggctggtaaa ctttcgatag tgcgagtatt
      301 gaatgatttc taggcgttat cgattttacg tattttttgc atgatagaat tcgtacctcc
      361 tcccaacgac cttccatggc tttccggact tctgtaggct ggcggacgat gctgtcctcg
      421 gatgttgtgc taattgggtg ataacagatc ctgtgtatgg gcgtttccct ttgtgcgtgc
      481 gttgatgctg gtaacggtaa acgtcatgat gaacacgacc gcgaataaga cgacaatcgt
      541 cggagctggt gcgctgtcga taaaaaagga cagccagaca ccagtcattg acactattac
      601 cgatatgcca gtcgccagta ggagcgcaat atgaaagcgt tgtgtcagga gtactgcaat
      661 ggcgccaggc gcaatgagca gagaaataga gagaatgata cctactgctt tcagcgtcgc
      721 cacaatggtc agggaaacca tgcacagcag cccataatgc agccatctcg tatgcaaacc
      781 gcttacctgc gcctgttgat aatcaaagct gaacagcaaa aaatcccgcc attttacgct
      841 aataacaagt gtaaccagcc cggcaataat caccgtctgg attatgtcgc cgatggttat
      901 cccgagcatg tcgccgaaaa gaatatggtc aagatgtacg tctggtttaa ttgcaatata
      961 caagatcaga cctgcggcaa acatgccaga aaaaacaatc cccataactg tatcttgctt
     1021 gattcggctg ttgtctttca ggtatcccgt cgccaccgcg cagaatactc cggcgacgaa
     1081 tgcccctgta gccagaggta accccagaat ccaggtaagc acgatgccag gaaaaaccgc
     1141 atgactcatc gcgtcaccca tcagtgccca acctttcagg accagaaaga ccgacagcag
     1201 cgcacagggt atggaaacta ccagtgaaat cagcagtgcg tgactcataa aggtgaactg
     1261 cagtggctcc agtaataatg ccatcatgag cgatcctcca gcgctttatg cgcacgccga
     1321 cgatttgcca aaagtccatg ggtgggcgca aatacaaatg ctaaaagaaa cagcagtgtc
     1381 tgcgccacca cgatgattcc acctgtcgcg ccgtccagaa agtaactgac ccacgcgccg
     1441 aggaaactgg ttacactgcc aatagttaca gcaattatga gcaggcgggg aaaacgatcg
     1501 gttaacaacc atgctgttgc accgggagtc accaccagac agatcaccag aaaggcacca
     1561 accgtttgca gcgccgcaac ggtcgaaacc gacagcaggg tgaagaagag gatttttaat
     1621 ctgcccggat gtaatccaat agcacgggcg tgattctcgt cgaaaaaggt caccataaga
     1681 tctttccatt tgaaaaaaag cacgatgatc gagagaatgc caatgatagt gagttgcagg
     1741 atatccgccg gatcgattgc cagaatattg cccaggacga tggtctgaat gttcacggat
     1801 gtcgggttca atgacaccat aaacagcccc aggccaaaaa aggacgagaa gatcaggccg
     1861 atgatggcat cttcttttaa gcgggtgcgt tggttaagaa acagcatgct gcccgctgcc
     1921 agtccgccgg aaaagaaggc tcccagagaa aacggaagcc ccagcatata tgcccccgca
     1981 acacccggga caatagagtg cgacagcgca tcgccaatca gcgaccagcc ttttagcatc
     2041 agatagcatg acaggaaagc gcagagaccg ccgaccattg ccgagaccca catcgcattg
     2101 agcatatatt catagctgaa gggttccagt agcacgttca tcttgcttcc ctctgcaccg
     2161 ctgacggtcg atgtgccaca aaagggcgtt catcgtcagt aataatgctt tcttccgagc
     2221 cgttaagtgt gacatggcgc agtacgccgc taaaagccag ctccaggttc tcggctgtaa
     2281 aagtggtgtc tgtcggcccg cttgccagga cggtgccttt gaccatgacc gtataatcgc
     2341 aaaatgtcgt gactgagccg agattgtgcg ttgagacaag catcgttttg ccttccgcgc
     2401 gtaactccct caacaggctg atgattttcg cttcggtttt cacatcaacg ccggtaaaag
     2461 gttcatccag caggatcaca tcaccctgct gtgcaattgc tctcgccaga aagacgcgtt
     2521 tcttttgtcc accggaaagc tcgccgattt gacgatggcg aaaatccacc atatcgacac
     2581 gttccagcgc atcggtgaca atctgacgat ccctcttttt ggctatacgc aacataccca
     2641 tatgcccata gcggcccatc atcaccacat cttccaccag tacaggaaat gaccagtcaa
     2701 cctcttctga ctgagggacg taggcaacca ggtttttctg tagcgcctgt cgcgtgggaa
     2761 tacccagaac agatatcttt ccgctggtca gacgcacaaa tcccataatc gctttaaaca
     2821 acgtcgattt tccggaaccg tttaccccaa ccagagcggc aatagatcca cttggtactg
     2881 tgaaggatgc atcgcgcagt gctgtgtgcc cgttacgcca ggtgacagta acatcattaa
     2941 cgacaatgcc tgcagattgc atcattattt ctcccttttc ccggctttaa ttccctgtac
     3001 cagcgtactg gtagtaacct taagaaggtc gatatacgta ggtaccgggc cgttttctgt
     3061 gctcagggaa tcgacataga gcacaccgcc gtagtgtgcg ccggtttcac gcgcaacctg
     3121 acgcgctggt ttatcggaaa tcgtactctc gctaaagact gccgggatat gatttttctt
     3181 aactatatca acaaccttac gtacctgctg cggtgttcct tgttgatcgg cattaatcgg
     3241 ccacagataa agctctttta gccccaaatc gcgtgcgaga taagaaaaag ccccttcact
     3301 ggtgaccatc catcgctgat tctcagggag ttccgtaatc tgcttacgca ggggggcaag
     3361 ggtttgggta atcttggctt tataagtatc ggcattacgt tggtaggttt gtgcatttgc
     3421 cggatcgtat tttatcaacg catcacgaat attatcgacg taaatcagag cattatctgg
     3481 cgacatccat gcatggggat taggtttgcc ctcatagggt ccttcggtga tccctactgg
     3541 cgtcacaccc gaagagacaa ttacttctgg aaccccattg agatgctggt aaaagcgttg
     3601 gaaccacaat tccagattca taccattggc gagaatcagt tgtgccccct gcgcacgttt
     3661 aatatcgcca ggggtaggct gatactcatg aatttctgca ccaggcttgg ttatggatga
     3721 gacttctgca gcatctccag ccacgttttt ggccatatcc gcgatgatgg taaatgttgt
     3781 aatgaccttg aatttttcag ttgcacttgc ctgaaatgcg atcgagcagg tgagggcgag
     3841 gcaccccaag agcatggtta ctttttttat tgagtgcata atttagtacc tatagtgagc
     3901 aaaattagaa caaacatagc aacggctatg ttttatagca taacctatac aaaatgaaaa
     3961 taattatcat taacagcttt gaggcctaaa acagggatta ggttgtttcc tgctggcgac
     4021 atgatattga ggaaaaaagt agagcagcta ttaggctttg aaatgacttt atgaggaaaa
     4081 ttgttgatgg caaaaatggg gcaaaaaaga gtgggttatc gtagcttatt gttgtcgctg
     4141 ataatgttta acatattgaa aaataaataa aatacttatg agtcagatag ttgtgatttt
     4201 tgcccttact tgttcaggtt gtattgttct ttctttgtaa tttgttgatt ttcttgcatt
     4261 atttcagttc tctggtacta aatggggcaa attgggggca aactttgcaa ctacgataac
     4321 cgcgcattca acatggctat ctgttcgtcg ttcatgtcat caatccacat accgtaaatt
     4381 tcatacacca tttgcgcagt ttcatgcccc atctggctgg ctataaatgc cgggttcgct
     4441 cctgccgtca acagccagca ggcaaaagta tgtcgcgtat ggtacggatt acggcggcga
     4501 ataccagcac gttttactgc tgcattccat cttgtaccca aactgcttac cgagtaataa
     4561 ggtttctgtt tttcgttaca caccctgggc atgaaaacaa aatgcagttt ttgtttttca
     4621 gttctgccgt actcccgatg ataaaaagtg atttcgcttt tgcgatgatg cccggtcagt
     4681 ttgtattgct ccttcagtgc ttcaagagcc ggctgcagta atgctaccgt ccggatcccg
     4741 gcatttgttt ttgggggacc gaacatatca agtatcgtca ggtttcttct gacattcaca
     4801 attcccttct cgaaatccac atcctcccac gccagagctg ccagttcccc gtgacgaagc
     4861 ccggagtaaa cggcaaattt ccacaagttt tggctctgtc ctttttcact ttccattaat
     4921 gcattga
//
LOCUS       DAJTQQ010000184         4908 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000184 DAJTQQ010000000
VERSION     DAJTQQ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.188"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            56..328
                     /locus_tag="OO309_003999"
     CDS             56..328
                     /locus_tag="OO309_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR5848932.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(294..701)
                     /gene="nudG"
                     /locus_tag="OO309_004000"
     CDS             complement(294..701)
                     /gene="nudG"
                     /locus_tag="OO309_004000"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR5848933.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            788..1417
                     /locus_tag="OO309_004001"
     CDS             788..1417
                     /locus_tag="OO309_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5848934.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1426..2733)
                     /gene="ynjE"
                     /locus_tag="OO309_004002"
                     /pseudo
     CDS             complement(1426..2733)
                     /gene="ynjE"
                     /locus_tag="OO309_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(2800..3453)
                     /locus_tag="OO309_004003"
     CDS             complement(2800..3453)
                     /locus_tag="OO309_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5848935.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3453..4879)
                     /locus_tag="OO309_004004"
                     /pseudo
     CDS             complement(3453..4879)
                     /locus_tag="OO309_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013366577.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT         1149 a         1339 c         1286 g         1134 t
ORIGIN      
        1 ttgccacccg gctaccgtta tcagtagcag agacaataac gttaaggagt tccctgtgag
       61 tcgagcattg ttcgccgtag tattagcatt tccattaatt gcactggcta acccgcatta
      121 tcgtccggac gtggaggtaa acgtaccgcc agaagtgttc agttcaggcg ggcagtcagc
      181 gcagccctgt actcagtgct gcgtttatca ggatcaaaat tattctgaag gggcggtaat
      241 taaagccgaa ggaatactgc tgcaatgcca gcgtgatgac aaaaccctta gcactaatcc
      301 gctgctctgg cggcgcgtaa agccataaac gcctctaata atggaatgtc agcaggggcc
      361 agcggatatt gcagcgcctc ttcaggtgag caccagacca gcgcctgatg ttcatgtgcc
      421 tgtaacgtcc cgtggaagtc gggtacgtgc caggcatgaa gatggataat ccggcccgaa
      481 acttctcgct gatggctggc aacatactca cccacagttg cttcgatgtc caattcttcg
      541 tttaactcac gcaccagcgc ctgctgctgg ctttcatcca gctcgacttt acctccggca
      601 aactcccata atcccgcctg atcgctctgg gcggggcgtt gcgcgagtaa aattttgcca
      661 tcacgttcaa tgatggcggc aacaacttca atcattttca taaattacac acgggcagat
      721 aagcgaaaaa tgccataata tccgcactgt tgctcaatca gaaaggcttt atatttaagg
      781 agtgcctgtg ctagaccgcc atcttcatcc ccggattaaa ccgttgttgc atcagtgcgt
      841 gcgggttctt gataaaccgg gcattacgcc agatggttta acattagtgg gatttgccat
      901 cggcgtgctg gctctgccgt ttctggcgct gggctggtat ctggcggcgc tagtcgtcat
      961 tttgttgaac aggctgcttg atggtctgga cggtgcgctg gcgcggcgca gagggcttac
     1021 cgacgcgggc ggctttcttg atatttctct cgattttctc ttttacgcgc tggtgccgtt
     1081 tggctttatt cttgctgcac cggagcaaaa tgcgctggca ggcggctggt tgttgtttgc
     1141 gtttatcggc acgggcagca gttttctcgc ctttgctgca ctggcagcaa aacatcagat
     1201 tgataacccc ggttatgcac ataagtcgtt ttactatctg gggggattaa ccgaaggcac
     1261 agaaacgatc ttactgtttg tgctgggatg tttattccct gcatggtttg cgtggtttgc
     1321 gtggtttgcg tggatcttcg gtgcgttgtg ctggatgacg acctttacgc gggtgtggag
     1381 tgggtatctg acgctgaagt cgctccagcg tcagtaattg caatgttatt tgctactgcc
     1441 cgggccgcgt tcaccggttg caaccggatt ttttggatcg ctgctccatt cgtaccagcc
     1501 gccgtcatac acggaaacat tcttccaacc catggcgcgt gcgtacataa aggtttcgga
     1561 cgcgcgccag ccggtgccgc agtagaatga aacttgctga tctggtttga tattccatgc
     1621 tttccacata gcggtaatat catcggcgct acgcatggtg ccatccgggt tatagaaatc
     1681 ttccatatgc gtcgagtcgc taccagcgtg tccccaacgt gctccggcta tttcaccttt
     1741 tggtttaata tagctgtaac cgctggtcgt accgataaat tctggccacg aacgaatgct
     1801 caccagcgat gcatcctggc gatgcagcag tccacgggcc tgttccatat cgagcatcaa
     1861 ctgcggttgt gccgggatct tcacgccgaa atccggttcc gctttcactt tcggtggcgt
     1921 tcctcgctca acaggcagtc ccgcgtcgga ccaggtttgc cagccgccat ccagcagacg
     1981 cacatctttc acgcctgcgt agagcataat ttgcgccaca cgcgctccag cgtatacgtc
     2041 acgcccgtac agaatgaccg tggtgtcatg gcgaatgccg tgttttgcca gcatcgcttt
     2101 tagttgttca tcagaaactt tgttccacag cggttcgctt tccacttcgt tggtatcgat
     2161 gtagtcagcg ccaggaatat ggctgataag gtaaagctta ggagcgccct aggccgcttc
     2221 aatgactttc cagtcaccga caggtttcgc cgtaacctct ttaccttgtt gcaggtcatg
     2281 cagccattgc ggataaacca gctgctcaaa gtgcggcagt ttttgtagac gggaaggttc
     2341 gcttagcgcg tcactcagaa tggagatatg cgtaaaacct gctttttgca gtcgcgtttt
     2401 gacggcgtca acatctttgc cattaccgta cagcgccacc ggggcatcgg ctttcaggtt
     2461 atgttgcttg atccacgcgt tgagctgttc ggtgctcatt ttgtccagcc agctagcaga
     2521 gaggtttaag gcggcaggtt catgaccaga agggccattt aaggtttgtg gccagccgtt
     2581 ataaaacgcg ctggggcgag tatctatcgc tttgccattt tgttgctgaa gttggtcaag
     2641 tgtaagaggc ttcgccagtt cagcggccca cgaagaagcg caagccagcc ctaaagccat
     2701 tgccagcgcg gtcatttgag aaacacgttt catcgaaaat cccgacattg tagttttgag
     2761 tgcgccattc tgaactgacg cattgagaaa aactttgcgt tatcgcagtt tgttgtaatt
     2821 ttctgaccac tgcgccatat ccagaacaga actatcagca ggaacatcct ggagatcgtg
     2881 cgttacctga acgacgggga tcgccagggc gcgaacttcg ctgaacaccc actggcgaaa
     2941 attatcgcgc agagccacat caagacggct gaatggctca tccaggagta acgcttttgg
     3001 ctgggcgaga agggcgcgta gtagagcaac gcgcgctcgc tgaccgccag acaaagtggc
     3061 aggatcctga tggaaagctc cgtcaaggcc tgaacgctca agtgcatcat tcacggcatt
     3121 acgtcgggca ttccccttaa gtgtcgccgg tagcgccagc agtaaatttt gcccgacgct
     3181 gaactggtca aataacagtg catcctgaaa aagaatgcca atctgacgct gtgcggtggg
     3241 tagcatgtca atccgttgct cattgagcca tagctcacct gtacaagaaa actgtccggc
     3301 cagtgcacca atcatccatg aaaacagagt ggattttcca cagccagacg gccccattaa
     3361 cgtgacgatg tcacctttat caaccgtaaa gttaacgttt gtcagcaagc ggctttctgg
     3421 taaacgtagc gaaacatttt tcacgcagag cattagcgga ttccttgtct gacataacct
     3481 acccattttg cgactaacgc ggtcagggca aaaataataa gcggtaacaa cagttgccat
     3541 aacgcctggg cggcgagaat accgttgctg ccgccgctgc ttaatgccac cgcctcagtg
     3601 gtgagcgtcg gaaaacgccc cgcgcccagc cacagcgtcg gcatatactg cgcaatactg
     3661 actgaaaatc ccaccgcgaa ggcaatcagc acagggcgca acatgagtgg gcatttcacg
     3721 taaaagaaga ttttggcccg cgaccagccc agtgtttgcg caatcaaaat taaccgtgaa
     3781 tcaatgcgct gccaggcggg ttgcaggata aacagcatcc acggcattac ccacagcaga
     3841 tgcccccaga ccaccgccgt ccagcttcca tccaggttca gccatagcgc cagcgtgtac
     3901 tggcccgcca ccagtggcag agcgggcaat agaatgggca accatagcca caactggcga
     3961 cgctgtgccc cccattccag ccacagcagt aacaggagca aagcgatgaa tgttgctacc
     4021 agccccattg tcagactgtt gatgagtgct tcactattga tcgtcgactg atccgcgagg
     4081 atcgccagca gaaccacaca cagcacaccg gttaagggta aaaaactcgc cagcgtagtg
     4141 cctggtaata aaggtgtggc aggcttgcga atgccatcta cgcggggaat agtgcgccgc
     4201 cagctgcgcc aaaacgtagg cggcgagtaa cagcatcagc agcagactgg cgagtgcgcc
     4261 tttcgtttgt tgatccgcgt cgccctgagt taaccactgc cagctaatta ctgccagcgt
     4321 cggtggatta cctggcccaa gaataatcgc cacatccacg accgaaagcg accaggcaac
     4381 aatcgccagc atcgccattg ccagcggctg tagcccagcg aatccagcac aatgacctgc
     4441 tgcaacagcc gtttttcgct caatactgcc actaagatcc acagcagaaa tgcgctttct
     4501 ttcactgcaa gggtgaggcc cagaccgatc caaatcggtc cattggcgga gtgaaatacg
     4561 ggaaatagtc atatagcagc cctccgtcag caaagagcag cagggcgctg gtggcaaaag
     4621 ccacatgggg aatggcgagc agccacggca gacgggcgca catacgctgc catttcggcc
     4681 caggccatag cgccacaatc accagcaggg caatcaacaa tgccccgacc gccgcgatgg
     4741 ttgtcgacac cagcgttgcc agtaatgcct gcggtaactg tggatcggca aataacgcct
     4801 gccagcgtgt caacgacaac gcaggcgata tcagcgtgag agctgccggg atcagcggtg
     4861 cataaatcac cgccaccatc gcccacagca aaaagattaa tgcgtacc
//
LOCUS       DAJTQQ010000185         4791 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000185 DAJTQQ010000000
VERSION     DAJTQQ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.189"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            121..1536
                     /gene="tnaA"
                     /locus_tag="OO309_004005"
     CDS             121..1536
                     /gene="tnaA"
                     /locus_tag="OO309_004005"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR5848936.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1627..2874
                     /gene="tnaB"
                     /locus_tag="OO309_004006"
     CDS             1627..2874
                     /gene="tnaB"
                     /locus_tag="OO309_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCR5848937.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3006..4181
                     /gene="mdtL"
                     /locus_tag="OO309_004007"
     CDS             3006..4181
                     /gene="mdtL"
                     /locus_tag="OO309_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR5848938.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4156..>4791
                     /gene="yidZ"
                     /locus_tag="OO309_004008"
     CDS             4156..>4791
                     /gene="yidZ"
                     /locus_tag="OO309_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR5848939.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQ"
BASE COUNT         1080 a         1159 c         1212 g         1340 t
ORIGIN      
        1 cgtttattta cttattttag taaatgatgg tgcttgtata tatacatggt gaattaatgg
       61 atattgcaga tgtaatattc acagggatca ctgtaattaa aataaatgaa ggattatgta
      121 atggaaaact ttaaacatct ccctgaaccg ttccgcattc gtgttattga gccagtaaaa
      181 cgtactactc gcgcttatcg tgaagaagca attattaaat ccggtatgaa cccgttcctg
      241 ctggatagcg aagatgtgtt tatcgattta ctgaccgaca gcggcaccgg ggcggtaacc
      301 caaagtatgc aggcagcgat gatgcgcggc gacgaagcct acagcggcag ccgcagctac
      361 tatgcgttag ccgagtcagt gaaaaatatc tttggttatc aatatactat tccgactcac
      421 cagggccgtg gcgcagagca aatctatatt ccggtactga ttaaaaaacg cgagcaggaa
      481 aaaggcctgg atcgcagcaa aatggtggca ttctctaact atttctttga taccacgcag
      541 ggccatagcc agattaacgg ctgtaccgtg cgtaacgtct atatcaaaga agccttcgat
      601 acgggcgtgc gttacgactt taaaggcaac tttgacctcg aaggattaga acgcggtatt
      661 gaagaagttg gcccgaataa cgtgccgtat atcgttgcaa ccatcaccag taactctgca
      721 ggtggtcagc cggtttcact ggcaaactta aaagcgatgt acagcatcgc gaagaaatac
      781 gatattccgg tggtaatgga ctccgcacgc tttgctgaaa acgcctattt catcaagcag
      841 cgtgaagcag aatacaaaga ctggaccatc gagcagatca cccgcgaaac ctacaaatat
      901 gccgatatgc tggcgatgtc cgccaagaaa gatgcgatgg tgccgatggg cggcttgctg
      961 tgcatgaaag acgacagctt ctttgatgtg tacaccgagt gcagaaccct ttgcgtggtg
     1021 caggaaggct tcccgacata tggcggcttg gaaggcggcg cgatggagcg tctggcggta
     1081 ggtctgtatg acggcatgaa tctcgactgg ctggcttatc gtatcgcgca ggtacagtat
     1141 ctggtcgatg gtctggaaga gattggcgtt gtctgccagc aggcgggcgg tcacgcggca
     1201 ttcgttgatg ccggtaaact gctgccgcat atcccggcag accagttccc ggcacaggcg
     1261 ctggcgtgcg agctgtataa agtcgccggt atccgtgcgg tagaaattgg ctctttcctg
     1321 ttaggccgcg atccgaaaac cggtaaacaa ctgccatgcc cggctgaact gctgcgttta
     1381 accattccgc gcgcaacata tactcaaaca catatggact tcattattga agcctttaaa
     1441 catgtaaaag agaacgcggc gaatattaaa ggattaacct ttacctacga accaaaagta
     1501 ttgcgtcact tcaccgcaaa actgaaagaa gtttaattaa tactacagag tggctataag
     1561 gatgttagcc actctcttac cctacatcct caataacaaa aatagccttc ctctaaaggt
     1621 ggcatcatga ctgatcaagc tgaaaaaaag cactctgcat tttggggtgt tatggttata
     1681 gcaggtacag taattggtgg aggtatgttt gctttacctg ttgatcttgc cggtgcctgg
     1741 tttttctggg gtgcctttat ccttatcatt gcctggtttt caatgcttca ttccgggtta
     1801 ttgttattag aagcaaattt aaattatcct gtcggctcca gttttaacac catcaccaaa
     1861 gatttaatag gtaacacctg gaacattatc agcggtatta ccgttgcctt cgttctctat
     1921 atcctcactt atgcctatat ctctgctaat ggtgcgatca ttagtgaaac gatatcaatg
     1981 aatttgggct atcacgctaa tccacgtatt gtcgggatct gcacagccat tttcgttgcc
     2041 agcgtattgt ggataagttc gttagccgcc agtcgcatta cctcattgtt cctcgggctg
     2101 aagattatct cctttgtgat cgtgtttggt tctttcttct tccaggtcga ttactccatc
     2161 ctgcgcgatg ccaccagcac cactgcggga acgtcttact tcccgtatat ctttatggct
     2221 ttgccggtgt gtctggcgtc atttggtttc cacggcaata ttcccagcct gattatttgc
     2281 tatggcaaac gcaaagataa gttaatcaaa agcgtggtat ttggttcgct gctggcgctg
     2341 gtgatttatc tcttctggct ctattgcacg atggggaata ttccgcgcga aagctttaag
     2401 gcgataatct cctcaggcgg caacattgat tcgctggtga aatcgttcct cggcaccaaa
     2461 cagcacggca ttatcgagtt ttgcctgctg gtgttctcta acttagctgt tgccagttcg
     2521 ttctttggtg tcacgctggg gttgttcgat tatctggcgg acctgtttaa gattgataac
     2581 tcccacggcg ggcgtttcaa aaccgtgctg ttaaccttcc tgccacctgc gttgttgtat
     2641 ctgatcttcc cgaacggctt tatttacggg atcggcggtg ccgggctgtg cgccaccatc
     2701 tgggcggtca ttattcccgc agtgcttgca atcaaagctc gcaagaagtt tcccaatcag
     2761 atgttcacgg tctggggcgg caatcttatt ccggcgattg tcattctctt tggtataacc
     2821 gtgattttgt gctggttcgg caacgtcttt aacgtgttac ctaaatttgg ctaaatcctt
     2881 caagaagcca gccattcgct ggcttcttgc ctctcaggaa atcacttatg tccaaatggc
     2941 aactcgcctt atcctccttc accacgtatg ctttgcgtca ccttactatc aggacgcttt
     3001 agcccatgtc ccgctttttg atttgtagtt ttgccctggt tttactttat cccgccggga
     3061 ttgatatgta cctcgttggt ttaccgcgca tcgccgccga tctcaatgcc agcgaagcgc
     3121 agttgcatat tgcgttctcc gtatatctgg cggggatggc agctgcgatg ctatttgccg
     3181 gtaaagtggc cgatcgttca gggagaaagc cggtcgccat acccggcgcg gcgctattta
     3241 ttattgcctc ggtgttctgt tcactggctg aaaccagcac gttatttctt gcaggccgat
     3301 ttctacaggg gttgggcgca ggctgttgtt acgtggtggc gttcgccatt ttgcgcgaca
     3361 cgctggatga tcgacgtcgg gctaaagtgc tgtcattact caacggtatt acctgcatca
     3421 ttccggtgtt agcgccagtg ctcggacatc tgattatgct taaattcccg tggcagagtc
     3481 tgttctgggc gatggcaatg atgggcatcg cggtactgat gttgtctttg tttattttaa
     3541 aagaaacgcg cccagcgtcc cccgctgctt cggacaaacc acgagaaaat agcgagtcgc
     3601 tgcttaatcg ttttttcctc agccgtgttg ttatcaccac cctcagcgtt tcggtgatcc
     3661 tcactttcgt caatacatcg ccggtattgc tgatggaaat catggggttt gagcgcggag
     3721 aatacgccac cattatggcg ctgaccgctg gcgtcagcat gaccttttca ttctcgacgc
     3781 catttgcgct gggaattttt aagccacgta cgttgatgat cacctcgcag gtgttattcc
     3841 ttgcggcggg gatcaccctt gccgtttcac cttcccatgc ggtttctctg tttggtatca
     3901 cgctgatttg cgccggtttc tcggtaggtt ttggcgtagc gatgagtcag gcgttaggac
     3961 cattttcatt acgcgcgggc gtagccagct cgaccttagg tattgcgcag gtttgcggtt
     4021 cgtcactgtg gatttggctg gccgcggtgg ttggtatcgg cgcatggaat atgctgatcg
     4081 ggattctgat tgcctgtagc atagtgagcc tgttgctgat tatgttcgtc gcgcctggac
     4141 gccccgttgc cgctcatgaa gaaatccatc accacgcttg atctcaatct gctgctctgt
     4201 ctgcaactgc tgatgcagga gcgcagcgtg accaaagcgg cgaagcggat gaacgtgacc
     4261 ccttcagcgg tgagtaagtc gctggcaaag ttaagagcgt ggtttgacga cccgctcttt
     4321 gtgaactcac cgctggggct gtcgcccaca cagctaatgg tcagcatgga gcaaaatctg
     4381 gcggaatgga tgcaaatgag caatctgctg ctggataaac cgcaccacca gacaccgcgc
     4441 ggcctgaagt ttgagttggc ggcggaatcg ccgctgatga tgatcatgct taatgcgctg
     4501 tcgaaacgga tctaccaacg ttacccgcag gcgaccatca aactacgtaa ctgggattac
     4561 gattccttag atgccattac tcgtggtgaa gtggatattg gtttttccgg tcgcgaaagc
     4621 catcctcgct cgcgggagct gttaagctcg ctaccgttag ccattgatta tgaagtgctg
     4681 tttagtgatg taccctgcgt ctggttacgc aaagatcatc cggcactgca tgaaacgtgg
     4741 aatctggaca cctttttacg ttatccgcat atcagcattt gctgggaaca g
//
LOCUS       DAJTQQ010000186         4781 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000186 DAJTQQ010000000
VERSION     DAJTQQ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.190"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..391
                     /locus_tag="OO309_004009"
     CDS             <1..391
                     /locus_tag="OO309_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCR5848940.1"
                     /translation="MTAGVYLRAFPRHIPAQNAVLDVTFEFPRGRATKDRLYFCVPML
                     DLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLLCYGHCKTVDEA
                     IIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(445..1050)
                     /gene="azoR"
                     /locus_tag="OO309_004010"
     CDS             complement(445..1050)
                     /gene="azoR"
                     /locus_tag="OO309_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR5848941.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1251..>4781
                     /gene="hrpA"
                     /locus_tag="OO309_004011"
     CDS             1251..>4781
                     /gene="hrpA"
                     /locus_tag="OO309_004011"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR5848942.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLRYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMT"
BASE COUNT         1180 a         1202 c         1321 g         1078 t
ORIGIN      
        1 aatgactgct ggtgtttatt taagggcgtt tccacgacat attccggcac agaatgcggt
       61 tctggacgtc acctttgaat tccctcgtgg acgagcgaca aaagatcgac tctatttctg
      121 tgtaccgatg ttggatctgg tggttccgga agagggggag ctccgacagg ccgtggcgat
      181 gctggaaaca ttacgcgaag agcaaggcag cgttctggtc cattgcgcat tgggattatc
      241 gcgcagtgcg ctggtagtgg cggcatggct gttatgttac ggacactgta aaacagttga
      301 tgaagcgatt atctttattc gagccagacg ctcgcatatt gtgcttaagg aagagcacaa
      361 agcgatgttg aaattatggg aaaacaggta agtggattga gatgtggact gaatatctac
      421 agtccacatc aagaccgtgt ccggttatgc agcaacaatg ctgtcgatgg ctgcttttgc
      481 gtcagactgt gctttcgctg ccatttccgg accgtatgcg atcccttcgg cgaagacaaa
      541 tttcacatcg gtaatgccga taaagccgag gaacgtggac agatacggcg tcaccaggtc
      601 cgttggtcca tctttgtgga tcccaccgcg gctggtaata acgatggctt ttttacccgt
      661 taccagacct tccggaccgt tctcggtata gcggaaagta acgcctgcgc gtgcaaccag
      721 gtcaaaatag tttttcaact gagttgagat gttgaagtta tacatcggtg ccgcaataac
      781 gataacgtcg tgggctttca gctcggcaat cagctcatcg gaaagcgcca gagcttcctg
      841 ctgacgcgga gtcagcggcg catcgctcgg acgcagagcg ccaaccagtt cgccatccag
      901 taccggaatc ggatttgcag ctaggtcgcg aacggtgatt tcatcagcgg agtgcttttc
      961 gctccattgt tcaacaaaat aatcggacaa ctgattagac tgagagtacc ctgccaggat
     1021 gctggattta agaactaata ccttgctcat ggtgtttcct tatagatgtt tgaatggacg
     1081 atgccccgtt gcttgctgac actttattca caatcctgcc acagagatcg cgcaataaat
     1141 cgaagtctat gttcgaattt attgaacaac gcatagaaag ccgcgatgtg gtactctata
     1201 tctatcattt aaaagaaaat taatcaggca gactactgcc cactaacgtt atgacagaac
     1261 aacaaaaatt gacctttact gccttgcaac agcggctgga ttcgctgatg ctgcgcgaca
     1321 gactgcgttt ttctcgccgt ctgcattgtg ttaagaaggt taaaaatcct gatgcacaac
     1381 aggccatttt ccaggagatg gcgaaagaga ttgaccaggc ggcagggaaa gtcctgctgc
     1441 gtgaagcggc acgaccggaa atcacttatc ctgacaattt accggttagt cagaaaaaac
     1501 aggacatact cgaagcgatt cgtgatcacc aggtggtgat cgtcgccggg gaaaccggct
     1561 ccggtaaaac gactcagcta ccgaaaatct gtatggagct ggggcgcggt attaaagggc
     1621 tgatcggtca tacccagccg cgtcgcctgg cggcacgaac ggtggccaac cgtattgcgg
     1681 aagagctgaa aacggagccg ggcggttgca tcggttacaa agtgcgtttc agcgatcacg
     1741 taagtgataa cacgatggtc aagctgatga ccgacgggat cctgctggcg gagatccagc
     1801 aagaccgcct gctgatgcag tacgacacca tcattattga cgaagcgcac gaacgcagcc
     1861 tgaatatcga ttttctgctc ggctatttga aagagttgct gccgcggcgt cctgacctaa
     1921 aaatcattat cacttccgcg actatcgacc cggaacgctt ttcgcgccac tttaataatg
     1981 cgccgattat tgaagtctcc ggtcggacct atccggtgga agtgcgctat cgcccgattg
     2041 ttgaagaagc cgatgacacc gagcgcgatc agttgcaggc gatttttgac gccgtagacg
     2101 aactaagcca ggaaagcccc ggcgacattc tgatctttat gagcggcgag cgggaaattc
     2161 gcgataccgc cgatgcgctg aacaagctga acttacgcca taccgaaatc ttgccgcttt
     2221 atgcgcggct ttcgaacagc gaacagaacc gcgtgttcca gtcgcacagc ggacggcgca
     2281 ttgtgctggc gaccaacgtc gcggaaacct cgctgaccgt accggggatt aaatacgtca
     2341 tcgaccccgg tacagcgcgt atcagccgct acagctatcg caccaaagtg cagcgtttgc
     2401 cgattgaacc catttcgcag gcgtctgcca atcagcgtaa aggccgctgt ggtcgtgtgt
     2461 ccgaagggat ctgtattcgt ctctattccg aagacgattt cctctcgcgc ccggagttta
     2521 ccgatccgga gattctgcgt accaacctgg cctcggttat tttgcagatg accgcgctgg
     2581 ggctgggcga tatcgctgcg ttcccgtttg tcgaagcacc ggataaacgc aatatccagg
     2641 atggcgtgcg tctgctcgaa gaactgggcg cgatcaccac ggatgaacag gccagcgcct
     2701 ataaactgac gccgctcggt cgccagctct cgcagttgcc tgtcgaccca cgtctggcgc
     2761 gtatggtgct ggaagcgcaa aaacatggct gcgtgcgtga ggcgatgatt atcacgtccg
     2821 cgctctccat ccaggatccg cgcgaacgtc cgatggacaa acagcaggca tcggacgaaa
     2881 aacatcgtcg cttccacgac aaagaatccg acttcctcgc gtttgtaaat ctgtggaatt
     2941 atcttggcga gcagcaaaag gcgctttctt ccaacgcctt ccgtcgcctg tgtcgtaccg
     3001 attacctcaa ctatctgcgc gtgcgcgaat ggcaggatat ctacacccag ttgcgccagg
     3061 tggtgaaaga acttggcatt ccggtcaaca gcgaaccggc ggagtatcgt gaaattcaca
     3121 tcgccttact gaccggtttg ctttcccata tcggcatgaa agatgccgat aaacaggaat
     3181 ataccggtgc acgtaacgcg cgtttctcca tcttccccgg ttctggttta ttcaaaaagc
     3241 cgccgaaatg ggtaatggtg gcggaactgg tagaaaccag ccgcctgtgg gggcgcattg
     3301 ctgcgcgtat cgacccggaa tgggtagaac ccgttgctca gcatttgatt aaacgcacct
     3361 acagcgaacc gcactgggaa cgggcgcagg gcgcggtgat ggcaacggaa aaagtcactg
     3421 tttatggttt gccgattgtt gctgcgcgca aggtcaacta cagccagatc gattcggcgt
     3481 tatgtcgtga actctttatt cgccacgctc tggtggaagg tgactggcag acgcgtcacg
     3541 cattcttccg tgaaaacctg aaactgcggg ccgaagtgga agagctggaa cacaaatcac
     3601 gtcgccgcga tattctggtt gatgacgaaa cgctgtttga gttctacgac cagcgcatca
     3661 gccacgatgt aatctccgct cgtcacttcg atagctggtg gaaaaaagtc agccgcgaaa
     3721 cgcctgattt gctcaacttt gaaaagagca tgttgatcaa agaaggggcg gaaaaaatca
     3781 gcaagctgga ttacccgaac ttctggcatc agggcaatct caagctgcgt ttgagatatc
     3841 agtttgagcc aggcgcggac gcggacggtg tgaccgtaca tattccgctg ccgttactta
     3901 accaggttga ggaaagcggg tttgaatggc agatccccgg tctgcgccgc gaattggtga
     3961 ttgctctgat taaatcgttg ccgaaaccgg tacgccgtaa ttttgtgcct gcaccaaact
     4021 acgccgaagc gtttttaggc cgcgtcacac cgctggagtt accgttgctc gacagccttg
     4081 agcgcgagtt acggcggatg accggcgtta ccgttgaccg cgaagactgg cactgggatc
     4141 aggtgcccga tcacctgaaa atcaccttcc gcgtggtgga tgacaaaaac aagaagctaa
     4201 aagaagggcg ctcgctacaa gatctgaaag atgcgctgaa aggcaaagtg caggaaacgc
     4261 tgtccgcggt ggcggatgac ggtatcgagc agagcggctt acatatctgg agttttggtc
     4321 agctgccgga aagttacgaa cagaagcgtg gcaactataa agtgaaggcg tggccggcgc
     4381 tggtggatga acgcgacagc gtgacgatca aactgtttga taacccgctg gagcaaaaac
     4441 aggcaatgtg gaatggtctt cgccgtctac tgctgctaaa tatttcctcg ccgatcaagt
     4501 atttacatga gaagttacca aacaaagcca agctggggct gtactttaac ccgtatggca
     4561 aagtgctgga gctgatcgac gactgtatct cctgcggtgt ggatcaactg atcgacgcca
     4621 atggtggccc ggtctggacg gaagaaggct ttgctgcgct gcatgaaaaa gtgcgcgccg
     4681 aactgaatga cacggtggtg gatattgcga agcaggtcga gcaaatcctc actgcggtgt
     4741 tcaatatcaa caaacgtctg aaagggcggg tggatatgac c
//
LOCUS       DAJTQQ010000187         4712 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000187 DAJTQQ010000000
VERSION     DAJTQQ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.191"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..708
                     /locus_tag="OO309_004012"
     CDS             <1..708
                     /locus_tag="OO309_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCR5848943.1"
                     /translation="YRNGTIQRQIAGFDEILRSPKLPAVTKSTATGVTVSGAQKFKPQ
                     AYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISFIGVKFLSQMAKNVLTDDATFSI
                     TRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTSLADTTPVNVLNVATTTANVFWA
                     DDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGIFATQGDINTLSGKCRIAVWYSA
                     CAVRPEAIGVGLPNQTA"
     gene            753..983
                     /locus_tag="OO309_004013"
     CDS             753..983
                     /locus_tag="OO309_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848944.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            961..1461
                     /locus_tag="OO309_004014"
     CDS             961..1461
                     /locus_tag="OO309_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCR5848945.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            1461..2879
                     /locus_tag="OO309_004015"
     CDS             1461..2879
                     /locus_tag="OO309_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCR5848946.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            2879..3727
                     /locus_tag="OO309_004016"
     CDS             2879..3727
                     /locus_tag="OO309_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848947.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            3727..4182
                     /locus_tag="OO309_004017"
     CDS             3727..4182
                     /locus_tag="OO309_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR5848948.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            4185..>4712
                     /locus_tag="OO309_004018"
     CDS             4185..>4712
                     /locus_tag="OO309_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR5848949.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAA"
BASE COUNT         1232 a         1161 c         1209 g         1110 t
ORIGIN      
        1 tatcgaaacg gtactattca gcgtcagatt gctggctttg atgaaattct tcgctcaccg
       61 aaacttccgg cagttaccaa gtcaaccgcg actggtgtaa ctgtttctgg tgcgcagaag
      121 tttaagccgc aggcatacac ccttgatacc gatggtaaca aagagaacgt cgacaaccgt
      181 gttgcaacgg tgaccgtatc ctccaccacc ggatttaagc gcggcgacaa aatcagcttc
      241 attggtgtga agttcctgtc tcagatggcg aagaatgtgc tgactgatga cgcgactttc
      301 tcaatcaccc gtgtgatcga tggtactcac atcgaaatca cgccgaagcc gattgcgctg
      361 gatgacgcgt cactgacaaa agaagagaag gcttacgcta acgtaaacac ctctcttgct
      421 gataccactc cggtaaacgt tctgaacgtg gcaacaacca ccgctaacgt gttctgggct
      481 gatgactcaa tccgcctgct gtctcagccg atcccggtaa cccatgaact gtttgctggc
      541 atgaaaacgt cttccttcag cattccaggt attggtgtta acggcatctt cgcaacgcag
      601 ggtgatatca acactctgtc tggtaagtgc cgtattgctg tgtggtattc agcatgtgct
      661 gtacgaccag aggcaattgg tgttggtctg cctaaccaga ccgcgtgata accagaggga
      721 gcttcggctc ccgtttttat ctggagacaa gcatgacaca catgatcttt cgtcatggcg
      781 acatgaagaa gtggaaaggc gttggctacg actttgaaat cgtgaaagcc gaagagcttc
      841 aggaatatct ggatgctggc tggttttcac atcctgatga ccttttgaag gatgttgcag
      901 agccagagcc agaagaaaaa cagcgtaaaa agcctggtcg aaaacctaag gcggcagcag
      961 atgaacctga caacgaaggg tgatttagtt cttgcggcat tacgtaagct cggtgttgca
     1021 tcaaatgcca cgttaaccga tgtcgaaccg cagtctatgg aagacggcgt caacgacctt
     1081 gaaatgatga tggctgaatg gcttggcggt gatgcgtcac ctggtatcaa cgttggctac
     1141 atttttgctg atgcagatgt tgctccggat ccgggcgatg agcacggttt atcaaataac
     1201 gctatcaatg ccgtcatttt caaccttgcc tgccgaattg ctccagatta tgcgctggaa
     1261 gcgtctgcaa aacttataac cactgccaga tacgggaaag agcgactcgt caaactgtct
     1321 gcaatggaca gggcaaaagc agctaaatgt aagtccggtt atccaaaccg tatgcctgtt
     1381 ggtagtggta accagttggc gaagtggaat ggttggaatt acttccaccg aaaggaacct
     1441 tgcgataacg ggagcgaata atgcctattc agcaacttcc gcttatgaaa ggtgtcggca
     1501 aagacttccg aaacgccgac tatatcgact atctgccagt gaatatgctg gctacaccca
     1561 aagaaatcct gaacagcagc ggatatcttc gctcattccc gggcattgcc aaacgttctg
     1621 acgtgaacgg tgtatctcgc ggcgtcgagt acaacatggc gcagaatgct gtttatcgcg
     1681 tgtgtggcgg caagctgtac aaaggagaaa gtgaagtcgg tgatgttgcc ggaagtggtc
     1741 gtgtatcaat ggcgcatggt cggacatcac aggcggtagg cgttaatggt caactggtcg
     1801 agtatcgcta tgatggcacg gtcaaaaccg tctcaaactg gcctacagac agtggattca
     1861 ctcagtatga gttaggttca gttcgcgaca ttacgcgctt acgtgggcgt tatgcgtggt
     1921 caaaagacgg aactgattca tggtttatca ctgaccttga agacgaatct catcctgacc
     1981 gttacagcgc acaatatcgt gccgagtctc agcctgacgg catcatcggt atcgggacat
     2041 ggcgagactt catcgtctgc tttggttcat cgactattga atatttctcc ctgactggcg
     2101 caaccaccgt tggtgccgct ttgtatgttg cacaaccatc gctgatggtg cagaaaggca
     2161 ttgccggaac ttactgcaaa acgccattcg ctgattcgta tgcgtttatc agcaatccgg
     2221 caacgggtgc gccgtctgtg tatatcatcg actccggtca ggtgtcacca attgccagcg
     2281 cgagcattga gaaaatcctc cgctcctaca ctgctgatga actggctgat ggtgtgatgg
     2341 aatcgctgcg atttgatgct catgagttgc tgattatcca ccttccgcgt cacgtcctcg
     2401 tgtacgacgc atcttcaatc gccaatggcc cacaatggtg tgtgctgaaa acaggtctgt
     2461 atgacgatgt gtaccgcgct atcgacttcg tttacgaagg caatcagata acgtgcggcg
     2521 ataagctgga atctgttacc gggaaactgc aattcgatat cagcagccag tacgacaagc
     2581 aacaggaaca cctgctgttt actccactct tcaaagcgga taacgccaga gtgttcgacc
     2641 ttgaggttga atcgtcaact ggcgttgcgc agtacgccga ccgccttttt ctctctgcaa
     2701 ccactgactg catcaattac gggcgtgagc agatgattga gcagaatgaa ccgttcgttt
     2761 acgacaagcg tgttttgtgg aagcgagtag ggcgcatcag gaaaaatgtc ggcttcaaat
     2821 tgcgcgttat cacgaagtca cctgtcactc tgtctggcgc tcagataagg attgagtaat
     2881 ggctgattcg aatctcaatg tgccggtaat catccaagct acgcggctcg atacatcagt
     2941 ccttccacgc aatatcttct cgcagtcgta tctgctttac gttatcgcac agggcactga
     3001 tgttggtaac gtggctaaca aagccaacga ggccggacag ggcgcttatg atgcacaggt
     3061 caggaacgat gagcaggatg tgattctcgc tgaccatgag cagcgaattt ctgctgcgga
     3121 agcaacgctt gttaatcatg aggagcgaat cagacaggca gaatcaactc ttcaggacca
     3181 tgaaacacga atagctcaga atgaaagcga tatttcgtcg cttgacacaa gagttcagtc
     3241 gctggagtcg caggtttcag accatgaaac gcgcatcgat gctctggagt atgcaaccac
     3301 acgcaagaag tcagaggttg tttactctgg tgtatctgta accatcccga cagcgccgac
     3361 caaccttgtt agcctgctga aaacgctcac accgtcatcc ggctcgttgg caccattctt
     3421 cgacaccgtt aacaacaaga tggttgtgtt caacgagaac aaaaccctgc tcttcaagtt
     3481 gtcgattgtc ggcacgtggc ccagcggaac cgccaacagg tcaatgcagc taaccttttc
     3541 cggctctgtt cctgacacac tggtcagcag tcgtaatgcg gcgacaacaa ccgacaacat
     3601 cctgttagct acgttcttca gcgtggataa agacggcttt cttgccacaa atggcagtac
     3661 gttaaccatt cagtcgaatg gtgcggcgtt tactgccaca accatcaaga taatcgcgga
     3721 gcagtgatga ttacattcaa accaacgcga aacatcgacc tgatagaagc agtcggaaat
     3781 caccctgaca ttatcgccgg gagcaacaac ggtgatggat acgactacaa acctgaatgc
     3841 cgttactttg aggtgaacgt gcacggtcag tttggcggca ttgtttacta tcaggagatt
     3901 cagccgctga cattagattg ccacgccatg tacctgccag agattcgcgg cttcagcaag
     3961 gaaattgggc tggcgttctg gcgatacatt ctgactaaca ccaccgttca gtgcgtaaca
     4021 tcgttcgctg cacgcaaatt ccgacacggt cagatgtact gcgcaatgat tggccttaag
     4081 cgtgtaggaa ccatcaagaa atacttcaaa ggcgtggatg acgtgacttt ttacagcgcc
     4141 acacgcgaag aactaatcga cttcctgaat cacgggagat agccatgtta tatgcattta
     4201 agctgggcag aaaactgcgc ggcgaggaac cttattgccc tgaaaaaggc gggaaaggtg
     4261 gcagttctga taaaagcgca aagtatgcag cagaagccca gaagtatgct gcagacctgc
     4321 aaaatcagca gtggcagacg atcatgaaaa accttgctcc gttcacgcct cttgcggagc
     4381 agtatgttaa ccagcttcag aacctttcca gtttagaagg tcaggggcag gcacttaatc
     4441 agtattacaa ctctaagcag tataaagacc ttgcagggca ggcgcgttac cagagtcttg
     4501 ctgctgcgga ggcgacgggt ggacttggtt cgacagccac aagcaatcaa ctggctacga
     4561 tcgcgccgac actcggtcag tcttggttat caaatcagat gagcaattac aacaatctgg
     4621 caaacgttgg gcttggtgcg ctgcaaggtc aggcaaacgc cgggcagacg tacgccaaca
     4681 acatgagcag cattgcacag caaagcgcag ct
//
LOCUS       DAJTQQ010000188         4660 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000188 DAJTQQ010000000
VERSION     DAJTQQ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.192"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..666)
                     /locus_tag="OO309_004019"
     CDS             complement(<1..666)
                     /locus_tag="OO309_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCR5848950.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
     gene            complement(617..1381)
                     /locus_tag="OO309_004020"
     CDS             complement(617..1381)
                     /locus_tag="OO309_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848951.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1576..2088
                     /locus_tag="OO309_004021"
     CDS             1576..2088
                     /locus_tag="OO309_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR5848952.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            complement(2177..2647)
                     /locus_tag="OO309_004022"
     CDS             complement(2177..2647)
                     /locus_tag="OO309_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR5848953.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAGEL
                     KLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(2644..3177)
                     /locus_tag="OO309_004023"
     CDS             complement(2644..3177)
                     /locus_tag="OO309_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5848954.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(3243..3872)
                     /locus_tag="OO309_004024"
     CDS             complement(3243..3872)
                     /locus_tag="OO309_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848955.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(3876..4091)
                     /locus_tag="OO309_004025"
     CDS             complement(3876..4091)
                     /locus_tag="OO309_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5848956.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            complement(4384..4458)
                     /locus_tag="OO309_004026"
     tRNA            complement(4384..4458)
                     /locus_tag="OO309_004026"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4423..4425),aa:Gly,seq:tcc)
     gene            complement(4462..4537)
                     /locus_tag="OO309_004027"
     tRNA            complement(4462..4537)
                     /locus_tag="OO309_004027"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4502..4504),aa:Thr,seq:tgt)
     gene            complement(4540..4616)
                     /locus_tag="OO309_004028"
     tRNA            complement(4540..4616)
                     /locus_tag="OO309_004028"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4580..4582),aa:Arg,seq:tct)
BASE COUNT         1154 a         1208 c         1042 g         1256 t
ORIGIN      
        1 tgccgcatca tcatcagact ccggaacgcc ctcatacaca tgtttaaaac catccggatc
       61 acggcgtcgc gccgcgtcga taaccttaag catggtgtca gacagaaacg gattttcgtc
      121 atagttaatt ttgcggatga gagtgccttc gggcggatca acaacgaagt tgcgccagac
      181 gaaatcagta acaagtccgg ggttgaatat gaaccagcat tccgaaccct ctttacggat
      241 cgttggctcc agaattttcc actggtattc cgtcagtgcg tgggcttctt ccaaccacaa
      301 catatcgatc ccctccagtg acttaatttc ttcgatgttg cgccataatc cataaaaaac
      361 aaattccgag ccagtaaccc ggttaatgat tttgttgttc agaatacgga aacggtgccg
      421 cagaccaaac ctgtctatct gaattttgag cagggtatac accgactctt cgattttatt
      481 ctggatctga cgggcacaac aaaaacgcag ggtgtattta ttcgacagaa atatggcaat
      541 gccagcggca tcccatgatt ttgacgatga ccgaccacca taaagcactt tgttacgtgc
      601 ccgcatagtc cagaaactac gtaaagccgg attcagcgtc ggtttggatg tcagagtaga
      661 agtcattgag gtcacgctct ccattgccat catcaatacc tgcatcacgg cgaagacgat
      721 cagcctccag agacacctta tcagtggcgg ccttgcggta ggctgtatca gcaaatattt
      781 ttcctaccgt cgcaagcgtg ccaacgatgg actcaatacg aactgtattg cgcatcattg
      841 ctttctcggc ggcgctgata ttttcaatca gcatcttcct ttcctggtcc ccattagcat
      901 cttccagcga caccaaccac cgaccaatat tctctgcagc gacaaggttg ttagcacgaa
      961 ggcgaaataa ttcgtcctcg agcgtcaacg ctttagcgtc ctctatcacc tcatctttga
     1021 gcagaaggcg acgggcataa ccaccgtgtt ttaatgcctg ctggttaccg ggttggaatg
     1081 ggttagtcgg gggatcggta cgcattccgc gtatcggttt cgtatccggt gtagattctg
     1141 tttttagttg cgtacttttt tgtgtaaggc cagtaatggc aggctttctg ctggtacgca
     1201 ctttcctttt ttgcgtacca tttttgcaaa cctgcgtacc gccactgcgt gcccaatcaa
     1261 gctttttggc cctcttcctg atagcccctt ctgtaacgcc gtatttctcg cctatatcac
     1321 ggaggctaag gactccggcc cggtatgccg attcgatggc ctcccagtcc ggttttgcca
     1381 tgattatgct tcctgtgatt tactgttatc gctgcctgtc acaataggtt gctttaattc
     1441 ttcctcatca ctcactcctg cgacgctccg tggcagggcc ccccacagga gtaccttgta
     1501 aatgtaaggt gtttaagcct aaattaagtc gtatcacggt taaatagcaa agaaaaattc
     1561 acagaaggaa agacattgat ggagtggata aatgctttga tgaactattt tgtacttcat
     1621 ccatatcgcc tatgggggct tctttttata atagcgttca caaaatccac tttactcatt
     1681 tcggcgatat tgccaccagc atcaattatg atggccactg taattacagt cagcaagacg
     1741 actctttcac catgggaagc tggaatcacg gtcatgagtg gcgcatggtg cggtactatc
     1801 gttaattacc atcttggtat aataattggg catatccctc aacttgcatg tataatatcg
     1861 agccgttcca atacaataga acgggttcgc cttcagttgc aaaataactc tgtgtcaata
     1921 cttttcactt cgcgctttat cgccgtgctg cgttacattg ctcctttggt ggcaggaatg
     1981 ttgcaacttc atccagtgaa agtgtataca gtgagtctga tttcagctgc tagctggtca
     2041 gcactttatg ttggtagttt tagcttcgtg ttgccttttt ttagctaaac tgcccataaa
     2101 aagctcttgc gaatccagct tcccccattc ctctgacaac agaatctgat ttgaaggata
     2161 atgagtaaaa ttaccgctac cccccaaggc attggatcct gatgtactcc tgaagcattc
     2221 tcaatgctgt ctggtcgctg atgattccgt cccggatacc gagaacgttt cgtccagcaa
     2281 ctggagagag ttcgacggtg gcatcattgc ccatgccgga ggcgctggag gtttcggctg
     2341 aggatggcac agggcatttt cctttgacga gcacccgacc accattatca agcttgcgcc
     2401 gaagagcatc attttcagct ttcgcatcag ctaactcctt cgtgtattta gcatcgagtg
     2461 catcagcatc acgctggcgc tgctgcatgt cagtaatggt tgcgttcgcc agcttcagtt
     2521 ctccggcgtt tttgtcgcgc tgctctttgt aggtaatggc gttatcacgg taatgattaa
     2581 cagcccatga caggcagacg atgatgcaga taaccagagc ggagataatc gcggtgactc
     2641 tgctcattga tctatccccc aacaggctaa tgcgctttcc tggtcacgac gaataacctg
     2701 tccatagcag ttatttgaac gtatgcggca atcgcgccca acatctttta tccaccagcg
     2761 aatcgcctcg catgcgccct tacgatcacc ggcattcagc cgcttataaa acgtcgacgg
     2821 gaaacactta ccggggccaa tgttataggg gcaaaatgac gctatacccg ctttctgggg
     2881 ttcggtcaga ggtactttaa tattgcgctc cacccatgcc agcgccttat cacgttcaat
     2941 agcgttaacc tggtcacatt tttccttcga cagcttcatt cccggtatga caggcttacc
     3001 atccaccatt gtggcaccac gacagatggt ccatatgcca gaaccatcac ggtatgccgt
     3061 tgtgtgatta ccctcttttt cgttcagaaa ctggtcaagt atctggggag cagacgcgcc
     3121 tgcggcaatc agcgccagaa cagcagccga caggccgtat ttgatttttg cgctcatgga
     3181 tatttatcag gatttatcgg caacaaataa cgagccaact tatatacgcc ttttaagata
     3241 agtcagcccc ggctggatcc agtcatcagg ctctttctta aagggggagt attgaaatca
     3301 cgaagaagag cctcccgcac cgcagcatcc atgtctgcac cactggcaag tgcttcaatc
     3361 tctgccgcca cctgcaaata ccccatgcaa cggccaatgc gtttcataac tctggtgttt
     3421 tcgtctttca ggcatctgat agcgagatta ataattttca ggcagtgatg atgcgatgcc
     3481 cggacaggtt ttccgttctc ataaacgacg aactcaccct cttcacagga aaccctgaaa
     3541 ggccgatggg gttcctggtc tttatccggt gcagacgcaa taaaatcact tttatgctct
     3601 tcctctccgg cactgttaac cgtaatgacg tactcaggcc gcaggtaagc cgcctctttt
     3661 tctgacaatg cggccggtaa aaacttttcc tgcaaaaact tcataaaagt ctcgtgagcg
     3721 actttgtggc agtaatcgta aatcgccgca taatgctcat cgcggcgttt gttttcatct
     3781 tcagaactca ccaacgccga caatttttta ttaagctcag tgatttcatt ttccaggtga
     3841 ttgaatcgct gattcatttc ttcatgcttc attatttact ctccccgtgc agccttacgc
     3901 ttgtcttctc ttattttgaa atacagattc gtcagataag tcagaagccc cagaagcaga
     3961 cttcccagca caccaatcgc agcccactgt gatggcctga cctgatccaa ccactgcaaa
     4021 aaccagtagc cggcactgcc ggcggaggtg ccgtaggcaa tgcccgttga aattttgtcc
     4081 atggatttca tagcctcacc tccgcaaata acggatggcg tagttttaca ctgagaaatg
     4141 aaagggattt gaaaagaaaa aaacgcaaaa gcgggcgaaa cgatatatac agtaaggaaa
     4201 gcactctatc caacaaacca cccacagtta atcggaataa aagcagagtg cttatgaatg
     4261 atcgcacgcc cgaaggttag tatttctgca cagcaatttt gcaaaaaaag gcgatcattc
     4321 ataacttaaa cgtctttcag tcactccggg atttcccatc atcgcagact gaaagactct
     4381 gactggagcg ggcagcggga atcgaacccg catcatcagc ttggaaggct gaggtaatag
     4441 ccattatacg atgcccgcat atggtgccga ctaccggaat cgaactggtg acctactgat
     4501 tacaagtcag ttgctctgcc tgctgagcta agtcggcgct ggcccaccac cgaggactcg
     4561 aacctcgcac cgtcaactta gaaggttgat gctctatccg gatgagctag tggtggttgg
     4621 tggcccttgc tggacttgaa ccagcgacct ggcgattatg
//
LOCUS       DAJTQQ010000189         4647 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000189 DAJTQQ010000000
VERSION     DAJTQQ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.193"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            89..1387
                     /gene="exuT"
                     /locus_tag="OO309_004029"
     CDS             89..1387
                     /gene="exuT"
                     /locus_tag="OO309_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR5848957.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            complement(1475..2563)
                     /locus_tag="OO309_004030"
     CDS             complement(1475..2563)
                     /locus_tag="OO309_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR5848958.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(2560..>4647)
                     /locus_tag="OO309_004031"
     CDS             complement(2560..>4647)
                     /locus_tag="OO309_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR5848959.1"
                     /translation="YDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNAQGG
                     NFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLGSFY
                     LNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQAGKT
                     TSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNLAIS
                     ASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYESISA
                     TVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSASRA
                     IVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTDYRY
                     SEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYATVS
                     DSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDEESQ
                     DLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAGNRE
                     LMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLEING
                     SEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQPKL
                     RGNVQTAQREVAP"
BASE COUNT         1100 a         1175 c         1121 g         1251 t
ORIGIN      
        1 cggttacccg gtcgtaacta acatcttcag cctctggcgg gatgatggcc gcgcttcctg
       61 cggatataac aaaacgatga ggttttacat gcgtaaaatt aaagggttac gttggtatat
      121 gatcgcactg gtgacgctcg gcaccgtgct tggttacctg acgcgtaaca ctgtggcggc
      181 agctgcgcca actctgatgg aagagttaaa catctccacc caacagtatt cctatatcat
      241 cgcagcctat tctgctgctt atacggtcat gcaaccggta gcaggttatg tactggatgt
      301 gctgggtacg aaaatcggtt atgcaatgtt tgctgtgctg tgggccgtgt tctgtggtgc
      361 aaccgcgctg gcaggtagct ggggtggcct agctgttgct cgtggtgcgg tcggtgccgc
      421 ggaagcagcg atgatcccgg cgggtctgaa agccagctcc gaatggttcc cggcgaaaga
      481 gcgttccatc gcagtaggct actttaacgt aggttcttcg attggtgcga tgattgcgcc
      541 gccgctggtg gtatgggcaa tcgtgatgca cagctggcag atggcattta tcatctccgg
      601 tgcattgagc tttatctggg cgatggcatg gctgattttc tataaacatc cgcgcgacca
      661 gaagcatctg accgatgaag aacgcgacta tattattaat ggtcaggaag cccagcacca
      721 ggtgagcacg gcgaagaaaa tgtccgttgg tcagatcctg cgtaaccgtc agttctgggg
      781 tatcgcactg ccgcgtttcc ttgcagaacc ggcctggggt acttttaacg cgtggatccc
      841 gctgttcatg tttaaagttt acggctttaa cctgaaagag atcgcgatgt tcgcctggat
      901 gccgatgctg tttgctgacc tcggttgtat cctcggtggt tacctgccgc cgctgttcca
      961 gcgttggttt ggtgtgaacc tgatcgtttc ccgtaagatg gtcgtaacgc tgggtgctgt
     1021 gctgatgatt ggcccgggta tgatcggtct gttcaccaac ccgtatgtcg caattatgct
     1081 gctgtgtatc ggtggttttg cccaccaggc actgtctggc gcgctgatta cgctctcttc
     1141 tgacgtgttc ggtcgtaacg aagtggcgac ggcgaacggt ttaaccggga tgtccgcatg
     1201 gctggcaagt acgctgtttg cgctggttgt cggtgcactg gctgacacca tcggcttcag
     1261 cccgctgttc gcagttctgg cagtatttga cctgctgggt gcgctggtta tctggaccgt
     1321 attgcagaac aaaccggcga ttgaggtggc gcaggaaaca cataacgatc ccgcaccgca
     1381 acattaacgt ttaacgtaag ttataacgac gaagccgcct tatcaggcgg cttcgtcgtt
     1441 tatatagaaa aaagcgtttg tccggtttgc agggttaatt gatggttggc aaggaaggcg
     1501 taaagattac tgacaatttg cccatgtagt acccggcctg tttatccatc acgctgaatt
     1561 tatcgactgc cagagtcaag ggtgtcggca cacacatcac ggcatagcga ataccgggca
     1621 gtaccaccgg acgcacacgt ttcaggttaa tgctgttcca gaccatattt tcattattgc
     1681 gcacatcaat ttgcccaccg gtttccgggt tgtacatttt gacccggtaa tcaatgcgtt
     1741 ctccctcgtc ggtggtggtc cctccctgac gatagataga gaagtaaccg tctctgcgcc
     1801 ctgcagctgg tcgcccttca tctttgagca tgatttcata actgatggca ttaccgttaa
     1861 aaccatcgta caggcacatg tccaacatgg tcagatcttg cgcgtagttg ctgccgttaa
     1921 cgcttcctgt tggatgcaag tctaactgca cccttggcgt ggcgtgggcg aactccggga
     1981 agaacacgtc aatatggttg gggtcgagcg tattcagggt aatatttgcg aaatagtcct
     2041 gggccgggct gttcgaatgt aatttaactt tccccttcca gacgccacca atcggcaaat
     2101 tcttcatctc tgtctgctca aggtagagcg taagaaactt tgtatcgtat atataataat
     2161 gttgaccatc ttctgtacat gaagatctat tagaatcctt aagattcgtc tgaagccctt
     2221 tgcccccctt gcatcctagc ggcgcttcat cgctacttac aatggctgtt ttaaatcctt
     2281 tgagttttac cggccaacgc atcccactgc gttgttcagt taaatagatt tgtactgtcg
     2341 atatatttcc attagctgta caggtttgtt ggaggtcagg atacgaacat tcataccaca
     2401 tatatccatt atttcctgcc gacgcttcat ccttccagaa gtaataatcc gctctaccag
     2461 atgccaaatc atacgtaatt tcactggtca cgtcaggagc tgcctgaacg ccagtgagcg
     2521 tgccaaacaa acccaatatc gccgcaatca atcgatttct catggtgcaa cctccctttg
     2581 cgccgtctga acgtttccac gtaatttagg ctgtttcaac tgtgcctgaa cctgaatcga
     2641 ctccggtaat tgtgaataag tcaccacact acaagtaaca tctccgctgt aacgaatgct
     2701 ggcgcgtttc tttacaacac tcagtggaca cttaaggaat tgctgcccgg aaagcagata
     2761 gagctctttt tcgctgccgt taatttcaag tgtaaatccg ccatcttcag ctaccagtaa
     2821 catggcgctg ttcagcccca caatcgggaa ttttttcacc gctgccggca gtaataaacg
     2881 accgatatag ttgtagttaa agctggcaga cacttcgcga tagcgcagct tgccaggcaa
     2941 caacattaat tcacgattcc ctgcaccgtt aatgatgttt gttgtcccct gctgattgcc
     3001 ctggctactg tcgttaacct caacaaaacc agactgaaat ccaggataag ggaacaacac
     3061 actgtcactt tgaccaagct gcaaagttcg gctgcctgcc acccgcgctt cgagttgcag
     3121 atcctgactc tcttcatcct gttcagagaa gcctttcaca tccaccagta ccgcacccag
     3181 caaatcgtct gtaccactgg ctcccaaatt aacgccatag cgactgacag ccagcgtaga
     3241 gctataagtg cccgtaaagg ccgaaagatg atcatgattc ttacggtcat aactatcaga
     3301 tacggtagcg tagacatttc cgtattgccc atcccaacgc ccgttaaccg cgctgtttat
     3361 ggtatcggtg ttcaggcctc cgacggttac gccaagttct ttatggctga atgaatcgtt
     3421 ataaaaggta tgcgataact gccatgacgt ttgatcgcca tcctgttcgc tataacgata
     3481 atccgtaggc acattggttg aatggctgtt gttattgcta tccatcgttg gcgtgtcaga
     3541 taacgaaaac gataagtaca agccgttgtc gtcgttatcg ccaccgttgt cattacgatg
     3601 gaaaacaccg actgacgtac tcaaaatcca gtcacgcatc acaatagcgc gggaagcgct
     3661 aagttgtaca gtttcagaga tactttgcgc tgttgtctgg cgaagatagt tattttcaaa
     3721 gctctgctgt ttatcccaac gcaaactatc atcagtgtta ttttgtgtgc aggtataacc
     3781 cagcatcata ttccagccga caaaattcag tgaaactgtc gcggaaatac tttcataaca
     3841 gcttaaggcg ttatagctct ctctgctgtc ggttgtacaa gcatcgccgt cagagttggt
     3901 ccgataaaag cccaacgtcg gccatcccgg attactccag ttggcctgtt gcatatcacc
     3961 tttgccgccg tcatcgttac ggaacacgct ggcgctgatt gcaagattcc ccaccctgcc
     4021 taaatctgcc gtccagttat tacctaactc gaaagcactc acatcatcag cattcgccag
     4081 ccctgcgtag agctcatatt gcggatgtaa tggcaggcgt actcctagct gataagctga
     4141 actttcatca tcagaaacct gtgatgtagt tttacctgcc tgtaagaacc attgcgcatt
     4201 tccgtcagtc agaccgccgg ttttggtaaa cggcacaagc tcggtgcggg tgagttggtt
     4261 attttcgtag acttttaatg ccacgctgta gctgcctggc ggaaaggaac tggtatcaat
     4321 aaattgcgaa ccactattga gataaaacga tcccaaaagt tgctcattac gataagcgtc
     4381 aacacgagaa ttgcgcgaaa gcagaaccat tactggggtt ccttgctggc tttgcgcctg
     4441 gtttaaatag ctcagtgtcg acccgatacg catcccatcg attgcaccga gtggcagaaa
     4501 gttaaaggta aagttcccgc cttgtgcatt aaatagtgtg cggttgtcca tgcgacccgc
     4561 ctgcacataa taacgacgta aaaaatcata acgataatag agatcgctga cgtcagtatt
     4621 actgtcactg acatcatctg catcata
//
LOCUS       DAJTQQ010000190         4613 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000190 DAJTQQ010000000
VERSION     DAJTQQ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.194"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..66
                     /locus_tag="OO309_004032"
     CDS             <1..66
                     /locus_tag="OO309_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5848960.1"
                     /translation="FSTVFKQRFGMTPGEYRRKFR"
     gene            complement(238..567)
                     /locus_tag="OO309_004033"
     CDS             complement(238..567)
                     /locus_tag="OO309_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR5848961.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(572..757)
                     /locus_tag="OO309_004034"
     CDS             complement(572..757)
                     /locus_tag="OO309_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR5848962.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(754..3393)
                     /locus_tag="OO309_004035"
     CDS             complement(754..3393)
                     /locus_tag="OO309_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR5848963.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3601..4590
                     /gene="ldhA"
                     /locus_tag="OO309_004036"
     CDS             3601..4590
                     /gene="ldhA"
                     /locus_tag="OO309_004036"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5848964.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
BASE COUNT         1152 a         1207 c         1066 g         1188 t
ORIGIN      
        1 ttttcaacgg tatttaagca acgctttggg atgacgccag gcgagtatcg acgtaaattc
       61 cgctaactaa ttggcgttgc atgtactgca acgccaatta gttaaaacag atatttgact
      121 atgcattttt cagggcgaaa ggcaatttga caggagtttt tgaatattca ccttgtttca
      181 ggaaaataga tcaatcgctt tctcctgtag cgttatcccg atattgcggt tttgctatta
      241 aaactttcgc acccatttac cgtttatccc ttgtacatat tcccccggtt tggcccgagc
      301 tacgagcttt tgcccggcga gtttcgcgat atcatctaca gacacattat tttgctttgc
      361 ccgttgttgg taactcgctt tacgttcggc attaatttcg gttactaatt tctctgtttc
      421 agcatccgtt ttcagcgcaa ccagataacc ataaaatgtt tcacctaccc gcccctgagt
      481 tctggcttca tccagagtca atgccattac attgctgctt accagcccaa caagaaacgc
      541 acaaagaagt aatgttttct tcatcatcgc ctcagaaaag atcgctacga gtttcaagca
      601 gctcttcgac atctttgtct gccttgatga tgatctcatg ctcaatttta acgttcatat
      661 tgatagtgat cggttcctta ggtgcagcga cttcaatgcg aggcgtacag ccaaccagca
      721 taaaagatga cgtcaacgca gccagtaaaa ttttcattgt ttttcctcac actcttttcc
      781 ttgcggacag tcatttcccg gcagacgtgc gttctgctcc aaccatgcct ggagattatc
      841 gccaaagcgt aaactgcgcc acagcgtaaa aatattctct tcatgatggt aattaaggtt
      901 taccgtaccg cttttaccat caacgcgact ggtacctgta atgttggcct gcatggttaa
      961 taaaccgaga ttatctaagt taatttttgt cgatgaccgg ctaatttcca tatagcgcaa
     1021 ccagttaatt gctgaacccg cagtcatatt gtctttcacc accgcatctg cggtatcttt
     1081 atccagccgc aaggtcatta gcccgctatt tgccagccaa ccatctttca ctatccattt
     1141 ttcgttgttt agccataacg gcaatgcacc actaaatgcc ccggacatgg cgaattgttt
     1201 cggattgacg gcgctaacca gttcgctgga tgagagatta ttcagccgca acagcgccgg
     1261 gtcatgttgc ggcatacgta attgtttcat cagcacgtta ccgcctaaca catcgacgct
     1321 aacatcagtc aacagcaagg gttcttcttc agtccacgga taacgccctt gcaaatcagc
     1381 cgtaatattt ttcgctgtca ccagattaat cacttcggca attcgcaacg taacggggcc
     1441 gcgagtcccc agatgccagg ctccatcggc aaaacggaaa ggcaggacaa aatcgacacc
     1501 gttaacctga ttatctggca tccaggcact accgcctttc aacacgccgt gccctcccgc
     1561 gcggaatcct tgttcaggcg cagcagaaaa tgcaacctga gcatacagtt caccatcgcg
     1621 taagttcatc ttccagtcgg gtggcaccag cggctggaat acagtcagtg attgttttgg
     1681 ccaccaggcg ttaccgcgca gacgaatacc gtcccagcga ccatttaccc gaaccgggcc
     1741 aatctcacca gcatgtaaat cgcctttaaa gaggaaatag gtcggattgc gcccatcgac
     1801 gctaaatttt aaggttgatg gcggtaacac actgccgcca gtgaacagcg tttgcccggc
     1861 gtccagtgac agcgcgccgc tgaaggaggg atgctgtgcg tcacgtaccc agacgacggg
     1921 cttgtcgaga attaatcgcg gcttttctac cgtcatcgta ccgtattgta actggtcgaa
     1981 accggtggag agatcggtca gcgtaatcgt gctgtcatgc cactcacctt ttcctgcgac
     2041 atcccaggtg gcattcatcg gtgtaaaact cccttttccc cagtagcgcc actgccagcg
     2101 gccagcgtca gggagaaaat cattcgccag cccatccata tgcagcacga aatcgcccag
     2161 ttcattttca tgcgcctgca aaatggcctg caaacgtccg tcaacaccac gttgggtgac
     2221 ttttacaccc gctaaaggcc agcggatttc atcgatgtcc agcgaatcga tgactcttcc
     2281 ctttgaacga agtaacgcgc ctggctcaaa ggtcagcgtt gggtcagaca gacttccact
     2341 tagctgcgca ggtaaacggg catataaaat gaggtccgcc tgtttcgctt caccggtcag
     2401 ctgcagaggc agttgactgt tatccatgct taattttcct gggccaaaat taagcaccgc
     2461 gttgccctta cccgcttgcc cctgggtcag cacattcagt cgtccgctga ccagagcgtt
     2521 ctcaagccct gcctgccagt tgtcgacttt gacacccagt cggccactca aaggaaaacc
     2581 agcatacagc cagctccagc gaccatcgct tacggtcaat tgttgacgag taatttgcca
     2641 cggcaaatcg agcaacggat cgccgttatc ccgtgccagc acgatcaatt gcccgctatt
     2701 ttcctgccag tccagctcgg catccaccag tgacggttcc tgcggcaagt ttaacgtcgc
     2761 agtagcatgc ccacttacag gaagtccatc cggcacgagc ggcatagtaa attcccccac
     2821 cagtttaacc ggcggctgat tttcaaacgc gacgacatcc agttcgctga ctgtaagttg
     2881 ttgccctttc agctggcctt gaaatttaac tttttcgccc tgataacgca gttgctggat
     2941 atcagaggtt aatgcgagag agagttttcc ctgccattcc tgccagggag aaaaaatcag
     3001 tttatcgata ttgatccagg tgttaggcag catggactgc cactgcgcga gggtttttgg
     3061 agcagctggc gattgctccg tctgcggcaa tttcgccaga caagcagaat caagttctac
     3121 cgtgccgacg ttcaataacc agcggctggg atgtgaaagg ctggcgttgg tgatatgcgc
     3181 aagctgacaa tctcccacca gataacggag atcgggaatg attaaacctt tacgcgtaat
     3241 gcgtgggctt tcatctaatg caatacgtgt cccgagcggt agccagatgt ccgccagcgt
     3301 gggaacccac agcccgagcg tcatcagcag cgtcaacggc acaagaataa tcagtaataa
     3361 cagcgcgaga acggctttat atttacccag catgggtagt taatatcctg atttagcgaa
     3421 aaattaagca ttcaatacgg gtattgtggc atgtttaacc gttcagttga aggttgcgcc
     3481 tacactaagc atagttgttg atgaattttt caatatcgcc atggctttca attatatttg
     3541 aaattttgta aaatattttt agtagcttaa atgtgattca acatcactgg agaaagtctt
     3601 atgaaactcg ccgtttatag cacaaaacag tacgacaaga agtacctgca acaggtgaac
     3661 gagtcctttg gctttgagct ggaatttttt gactttctgc tgacggaaaa aaccgctaaa
     3721 actgccaatg gctgcgaagc ggtatgtatt ttcgtaaacg atgacggcag ccacccggtg
     3781 ctggaagagc tgaaaaagca cggcgttaaa tatatcgccc tgcgctgtgc cggtttcaat
     3841 aacgtcgacc ttgatgcggc aaaagaactg gggctgaaag tagtccgtgt tccagcctat
     3901 gatccagagg ccgttgctga acacgccatc ggtatgatga tgacgctgaa ccgccgtatt
     3961 caccgcgcgt atcagcgtac ccgtgacgct aacttctctc tggaaggtct gaccggcttt
     4021 actatgtatg gcaaaacggc aggcgttatc ggtaccggta aaatcggtgt ggcgatgctg
     4081 cgcattctga aaggttttgg tatgcgtctg ctggcgttcg atccgtatcc aagtgcagcg
     4141 gcgctggaac tcggtgtgga gtatgtcgat ctgccaaccc tgttctctga atcagacgtt
     4201 atctctctgc actgcccgct gacaccggaa aactaccatc tgttgaacga agccgccttc
     4261 gatcagatga aaaatggcgt gatgatcgtc aataccagtc gcggtgcatt gattgattct
     4321 caggcggcaa ttgaagcgct gaaaaatcag aaaattggtt cgttgggtat ggacgtctat
     4381 gagaacgaac gcgatctgtt ctttgaagat aaatccaacg acgtgatcca ggatgacgta
     4441 ttccgtcgcc tgtctgcctg ccacaacgtg ctgtttaccg ggcaccaggc attcctgaca
     4501 gcagaagctc tgaccagtat ttctcagact acgctgcaaa acttaagcaa tctggaaaaa
     4561 ggtgaaacct gcccgaacga actggtttaa tcttgcagct cccctgcaat cca
//
LOCUS       DAJTQQ010000191         4592 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000191 DAJTQQ010000000
VERSION     DAJTQQ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.195"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(78..866)
                     /gene="phoH"
                     /locus_tag="OO309_004037"
     CDS             complement(78..866)
                     /gene="phoH"
                     /locus_tag="OO309_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR5848965.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="OO309_004038"
     CDS             complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="OO309_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR5848966.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="OO309_004039"
     CDS             complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="OO309_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR5848967.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3929..>4592)
                     /locus_tag="OO309_004040"
     CDS             complement(3929..>4592)
                     /locus_tag="OO309_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR5848968.1"
                     /translation="NETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQ
                     LEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGL
                     ATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIA
                     FDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASR
                     SA"
BASE COUNT         1077 a         1294 c         1107 g         1114 t
ORIGIN      
        1 ttcagaaaaa gcgctagtcc gggcataaac acccaaaaca aagcccggtt cgcccgggct
       61 ctgcaccgat aatacactta actgtatgca tgcagcgtac gttggcaaag tgccgaacgt
      121 acgcaatcct cttttccgaa ccggacgatc ccaaccattt catcttcttc gaaacgttcc
      181 agcgcgtcac ttaatccgga gcacacgccg cgaggcaaat cgcattgcgt gatatcaccg
      241 ttgacgataa ccgtcacgtt ctccccgagg cgggttaaaa acattttcat ttgcgcggca
      301 gtcacattct gcgcctcgtc aagaatgaca actgcatttt caaaggtacg tccacgcata
      361 taggcgaacg gcgcggtttc caccttccct atttccggtc gcaggcagta ctgcataaag
      421 gaagccccta agcgccggac cagcaagtcg tagaccgggc gaaaataggg agcaaacttt
      481 tctgcgatat caccaggtaa gaagccaaga tcttcatcgg cttgcagaac tggacgggtg
      541 acgataatcc tgtcgacatc cttatgtatc agggcctctg ccgcttttgc tgcgctgatc
      601 caggtttttc cgcacccggc ttcgcccgtg gcgaatatca gctgcttact ctcaatagcc
      661 tgcagatagt gcaattgcgc ttcatttcgc gcaaggatgg gcgaggtatc gcgactgtcg
      721 cgggccatac caatggcttc tacgccgctc atctgcacaa gcgaggtgac cgattcttct
      781 tcacgctgct tatggctgcg cgaatcccgt ctcagcacac gttttgcctc gcgacgagct
      841 ttgatcactg ctttttgtct tcccatggag agcaccttga gttgtttgta ttcatcacac
      901 gcgccgttgg cagcgcgatt atgcgcacga acatcagagg gttggcttcc ttgtaagcca
      961 tagtttgctt ttggataaaa tgccgaaaaa cggctacgca caccgtttgc ggcgtcggta
     1021 acacgagtaa agaaaggaag tgagaaaaat acagagtgac gaagttgcta ccggaggaga
     1081 aatctccgcg agtggtggag cgttgcttaa caaaacattg tccagaacag gacgttacca
     1141 tccgcgatct ccatagtgac tgactatcac tgccgggaac ttccgctgct acttaataag
     1201 tacaacagaa ctcgcattca ttgcaacaat atattcactt atatttaacg ataacaccca
     1261 tttcggtgac attaatttct actggaaaga tgacagagtg atgacagtga tggaaaaagc
     1321 tgtgtgcttt ccgcaggatt cgcagggctg gtaaaatgtg cctcgtgttt gccggattcg
     1381 gcgcgaacgc cttacccgac ctacaagtga ttaataattt gtgcacaaaa ctcatgcccg
     1441 ataaacctgc gcatcgggca tatttgcgtc gttcaaacct gcaatagcgc ccttcccaga
     1501 taatcgttcg cgtccttcac tcccggcagc gaaaaaaaat aaccgccgcc gataggttta
     1561 acgtattcct ccagcgcttc gccattgagc cttttttgta ctgtcaggaa gcctttttcc
     1621 agatcgtgtt ggtagcagac aaacagcaac cccatatcca gttgtccgga gttggtgacc
     1681 cccaacgaat aactgtagcc gcgacgcagc atcaggctgg actcactctc cgccgtgcgg
     1741 ggattcgcca gccggatatg gctgtccagc gcgatcacct tcccttccgg gtcgctggcg
     1801 taatcaggca catcatgctc atgctgcatt cccagcggcg caccggtttg cttatcacgg
     1861 ccaaaaatcg tctgctgttc tttcagcggc gttctgtccc aaaattccac tcgaaactga
     1921 atcaagcgta ctgcctgata gctgccaccg attgtccacg caggctcctg ctgatctgcc
     1981 gttacccaca ccactttttg catcaactta tcattctggc tatcgggatt ggcagtgccg
     2041 tctttgaaac ccagcaaatt aatcggcgtc tctttgcctt tactacgcgc cgcgtgatcg
     2101 gaaataaacc cttcccgctt ccagcgcaca ctgagcaaat ccggcgtgtg tttgatgata
     2161 tcgcgcagcg catggttcac cgtgtcctgg gtgttggcgc aaatctgtag caacacatca
     2221 ccatgacata acgccgcatc cagcgagtcg ttggggaaac gcgtcatctt ctgcagcttt
     2281 tttggcatct gtggcgcaag gccaaagcgc tcatcaaaca atgagtgacc caccgataac
     2341 gtgatggtga gattatcggg cgcaatgtag ccgccaagaa tgccggaatc gagtggtggc
     2401 aggcgcggat ttggcgtttc tggtgctgct ccgccctgac tcagaaaagc aaaacgctga
     2461 gtcaacaagc gaaacaaccg ctcaagatcg gctttatcgc tggcaagcac atcaaacgcc
     2521 accagcatca ttgcggcctg ttgtggcgtc aggatccctg cctgatgctc accataaaac
     2581 ggctgtttct cattgcgtgc atccggtgaa agcgtgcccg gtgcactttg cgttttttgt
     2641 gcatgagcga ccggacaact tcccgccagc gcaccaatcc ctttcagtaa acgtcggcgt
     2701 gacggttcat tcacgccgtt ttcatcttta tactgcataa cgcttaatcc agtcccagca
     2761 caccgcgaag ttgcgccaga tcttccgcca gcgcagtaat cggccctttc agcgcattcc
     2821 ggtcggcatc ggtcaatttg tcgtaggttt caaaaccgtc tttagtacgg tatttcgcca
     2881 gaatggtatc gaccttttta aagttggcat cgacttttgc caacagttcc gggttggctt
     2941 tttgcagttg tggacgcagc agatcgacaa ttttctgcga gccttcaacg ttagcctgga
     3001 aatcccacag atcggtgtgg ctgtagcgat cttcttcacc gctgattttg ctggctgcca
     3061 cttcctcaat cagtccggct gcgccgccga ccacttttga aggtgggaaa gccagttcac
     3121 tgatgcgttt ttgcaaatcg accacatcgg tataaagctg ctcagcgtac tgatccatcc
     3181 ctttggtggt gttgtcgcca aacaatgctt tttccagacg gtggaaacca gtgaattttg
     3241 ggtcggcggc tttttgctcg taatcatctt cacgggcgtc aatgctgcca tccagatcgg
     3301 agaacagttc agcaatcggt tcaatacgct catagtgctg gcgcgtcggc gcatacagtg
     3361 ctttcgcttt ttcgatatcg cctgctttaa tcgcgtcggt aaaggctttg gtgtcggtca
     3421 ccagctgcgt ggtttccgcc atgacatacg ctttatatgc agtaattgca ccaccaagac
     3481 ttaacagcgc atcactttgc gccgcatccg ccgttgcctc acctttgacg atcaacttcc
     3541 ctttcgggtt agtcagcaga ccgcaggtca tatcgtattc gccaggctgt aaattcgccg
     3601 tcattttctg gctaaaacca ggggcgatat tttcccgctc ttccaccacc atcacgcctt
     3661 tgaggatctc ccactccagc gccttctggc tatggttctg aataatgaac tgtgttttcc
     3721 cggcgttaac cgtaatggtc atcggttcgc actgcttatc cgtcacggtc actttgacct
     3781 gcggcacatc agcggcgtta gccataaaag cagaagaaaa cagcgcagcc acgctcaact
     3841 gcaatgcgtt acggcggaag ttaatggtca tgacgagact atccctttaa agtatgttgt
     3901 aactaagcaa gagtttgcgt cgtatttgtt acgcggagcg agacgctgtc gcccctgcgc
     3961 gtggtggcag agcaaatgcc accagcgccg ggatgagata aataaaccag acggcgactt
     4021 cgctgacgct cggtgcttcc tgatagccaa aaatcccttc catcagcgtg ccaaacagcg
     4081 agtgagttga gaggaccgcg ctcatatcga aggcgatttc ctgaaagtgg ttccacaatc
     4141 cggcttcatg aaatgcgcga atggcaccag ccgccagccc tgcggcgacg aagagaataa
     4201 acaggctggt ccatttaaaa aatgcaccaa gattgaggcg aataccgccc cagtagagca
     4261 ggaagccgag caccacggca gtagcaagac cgagcattgc acccagcggc ggccagatcc
     4321 cgacatcttg ttgaaatgcc gccagcagga aaaagaccga ctccagccct tcccttgcaa
     4381 cggcaaaaaa gaccatcatc accagcgccc agccatgatg atttccacgc tgcaatgcgc
     4441 tatcgactgc ctgttccagt tgcactttga cgttgcgcga cactttgcgc atccagaaaa
     4501 ccatccaggt aaggatcacc acggcgatca ccgccacgat accttcaaac agttcctgct
     4561 ctttttgcgg aaattcgccg gtggtttcgt ta
//
LOCUS       DAJTQQ010000192         4475 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000192 DAJTQQ010000000
VERSION     DAJTQQ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.196"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..508
                     /locus_tag="OO309_004041"
     CDS             <1..508
                     /locus_tag="OO309_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR5848969.1"
                     /translation="ATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITLLNQAVE
                     RVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQ
                     GLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIE
                     AMESRSLR"
     gene            705..2144
                     /gene="aldA"
                     /locus_tag="OO309_004042"
     CDS             705..2144
                     /gene="aldA"
                     /locus_tag="OO309_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR5848970.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2186..3186)
                     /gene="gap"
                     /locus_tag="OO309_004043"
                     /pseudo
     CDS             complement(2186..3186)
                     /gene="gap"
                     /locus_tag="OO309_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            3375..3905
                     /gene="cybB"
                     /locus_tag="OO309_004044"
     CDS             3375..3905
                     /gene="cybB"
                     /locus_tag="OO309_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR5848971.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4150..4323
                     /locus_tag="OO309_004045"
     CDS             4150..4323
                     /locus_tag="OO309_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848972.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(4395..>4475)
                     /gene="hokB"
                     /locus_tag="OO309_004046"
     CDS             complement(4395..>4475)
                     /gene="hokB"
                     /locus_tag="OO309_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCR5848973.1"
                     /translation="TRQTLYELRFRDGDKEVAALTACTSR"
BASE COUNT         1140 a         1032 c         1125 g         1178 t
ORIGIN      
        1 agcgaccatc ctggtggata tcgctcatca gttctggcat attccgcatg aaaaaatctg
       61 gattgaagac cagtcaacaa actgcggtga aaacgcacgc tttagcatca cgctattgaa
      121 tcaggccgta gaacgagttc atacggctat cgttgtacag gaccccacca tgcagcggcg
      181 cacgatggca acgttccgcc gtatgaccgg ggacaatccc gatgcaccac gctggttaag
      241 ttatcccgga ttcgttcctc agttaggaaa taacgcagac agtgtaatct ttattaatca
      301 gttacaagga ttatggccgg ttgagcgtta tctctcacta ctcactggcg agctgccgcg
      361 tttacacgat gatagcgatg gctacggtcc ccgcgggcga gattttatcg ttcacgttga
      421 ttttccggca gaagtcatcc atgcatggca aacgctgaaa catgatgcgg tgctcatcga
      481 ggcgatggaa agtcgctcgt tacgttaaaa attgcccgtt tgtgaaccac ttgtttgcaa
      541 acgggcatga ctcctgactt ttatttctgc cttttattcc ttttacactt gtttttatga
      601 agccctccac agacttgtcc tttcccgatg ccgtctctct gatgattgat gttaattaac
      661 aatatattca ccgaaaacaa acatataaat cacaagagtc gcccatgtca gtacccgttc
      721 aacatcctat gtatatcgat ggacagtttg ttacctggcg tggagacgca tggattgatg
      781 tggtaaaccc tgctacagag gctgtcattt cccgcatacc cgatggtcag gccgaggatg
      841 cccgtaaggc aatcgatgca gcagaacgtg cacaaccaga atgggaagcg ttgcctgcta
      901 ttgaacgcgc cagttggttg tgcaaaatct ccgccgggat ccgcgaacgc gccagtgaaa
      961 tcagtgcgct gattgttgaa gaagggggca agatccagca gctagctgaa gtcgaagtgg
     1021 cttttactgc cgactatatc gattacatgg cggagtgggc acggcgttac gagggcgaga
     1081 ttattcaaag cgatcgtccg ggagaaaata ttcttctgtt taaacgtgcg cttggtgtga
     1141 ctaccggcat tctgccgtgg aactttccgt tcttcctcat tgcccgcaaa atggctcccg
     1201 ctcttttgac cggtaatacc atcgtcatta aacccagtga atttacgcca aacaatgcga
     1261 ttgcattcgc caaaatcgtc gatgaaatag gccttccgcg cggcgtgttt aaccttgtac
     1321 tggggcgtgg tgaaaccgtt gggcaagaac tggcgggtaa cccaaaggtc gcaatggtca
     1381 gtatgacagg cagcgtctct gcaggtgaga agatcatggc gactgcggcg aaaaacatca
     1441 ccaaagtgtg tctggaattg ggtggtaaag caccggctat cgtaatggac gatgccgatc
     1501 ttgaactggc agtcaaagcc atcgttgatt cacgcgtcat taatagtggg caagtgtgta
     1561 actgtgcaga acgtgtttat gtacagaaag gcatttatga tcagttcgtc aatctgctgg
     1621 gtgaagcgtt gcaggcggtt caatttggta accccgctga acgcaacgac attgcgatgg
     1681 ggccgttgat taacgccgcg gcgctggaaa gggttgagca aaaagtggcg cgcgcagtag
     1741 aagaaggggc gagagtggcg ttgggtggca aagcggtaga ggggaaagga tattattatc
     1801 cgccgacatt gctgctggat gttcgccagg aaatgtcgat tatgcatgag gaaacctttg
     1861 gcccggtgct gccagttgtc gcatttgaca cgctggaaga tgctatctca atggctaatg
     1921 acagtgatta cggcctgacc tcatcaatct atacccaaaa tctgaacgtc gcgatgaaag
     1981 ccattaaagg gctgaagttt ggtgaaactt acatcaaccg tgaaaacttc gaagctatgc
     2041 aaggtttcca cgccggatgg cgtaaatccg gtattggcgg cgcagatggt aaacatggcc
     2101 tgcatgaata tctgcagacc caggtggttt atttacagtc ttaatgagtg aaagaggcgg
     2161 aggttttttc ctccgcctgt gcgcgtcaga gtttagcgaa tttttcgagg gtgcgaataa
     2221 gctgcgtgac gaagccatat tcgttatcgt accaggcgac cgttttcacc agttgtaaat
     2281 cgcccacggc ggtaatttcc gtttgcgtgg catcaaacac tgaaccgaaa tggctgccaa
     2341 tgatatcgga agagactatt tcttcatcgg tataaccaaa tgactcgtta ttggtggttg
     2401 cttgtttaag tgcgttattc acctcttcgg cagtcacttt tttccgagaa tggacaccag
     2461 ctcagtgacc gaacctgttt tcaccggcac gcgttgcgca tgacctttca gtttgccgct
     2521 cagttccggg atcaccagac caatggcttt tgccgccccc gtagtgtggg gaatgatatt
     2581 ttctgccgct gcgcgtgaag cacgtaaatc tttaccacgc gggccatcca ccagtgactg
     2641 ggtgccagta taggcatgaa tggtcgtcat cgtgccgact tctatcccga aactgtcatg
     2701 caaggctttg gccatcggcg caaggcagtt agtggtgcat gacgccacgg aaacaatggt
     2761 gtcgttgcca tccagagtgt cgtcattaac gttataaacg atggttttca tttcaccggc
     2821 aggagcggaa atcaacacct tcttcgcacc agcatcaaga tgcgcctgcg atttatcggc
     2881 ggaggtataa aagccagtac attcgacaat gatttctgcc cctttcgctt tccacggaat
     2941 atttttagcc tctttttcgg cgtaaaccgc gatacttttc ccatcaacga taagtgaatc
     3001 ttccgtaaaa tcaacgctcc aggggaacgg accatagttt gaatcatgtt tcagcagata
     3061 ggcgagaatt tttggggaag taagatcatt aatagcgaca acgtctatgt tgcttttgac
     3121 ttcaagtaaa cgacgcaaca ccagtcgacc gatacgacca aaaccgttaa taccaacttt
     3181 actcatggtt ttctcctgtc aggaacgttc ggatgaaaat tgatcctttc caggcttaga
     3241 ccaggattgt gggatgggca atctccattc ttacagtgaa acgtaacgta ctgaaaacgg
     3301 gtgaacaata tttaatgaaa ttttgagaaa agcccgttat gttaacggaa aattatgtta
     3361 aagcaggaaa tgttatggaa aataaatact caaggttaca aatcagcatt cactggctgg
     3421 tctttttgct ggttatcgca gcgtattgcg caatggagtt tcgtggtttc ttcccacgta
     3481 gcgatcggcc gctcatcaac atgattcatg tttcctgtgg catctcaatt ctcgtgctga
     3541 tggtggttcg tcttttgtta agactgaaat acccaacccc gccgattata cctaagccga
     3601 aaccgatgat gacgggactg gcgcatttgg gacatttggt gatttatctc ctgtttattg
     3661 cgctgccagt gattggtttg gtgatgatgt ataaccgggg caacccgtgg tttgcgtttg
     3721 gtttgacgat gccttacgct tcagaggcca atttcgaacg ggttgatagc ttaaagtcgt
     3781 ggcatgagac gctggcgaat ctgggatatt ttgtcatcgg gttgcacgct gcggctgcac
     3841 tggcacacca ctatttctgg aaggacaaca cacttctacg catgatgccg cgtaaacgtt
     3901 cctgaaggat atttaaagaa aacgcctgta ctaaaaccga cccgtggtac aggcgaagaa
     3961 tacgggtcta catcggaagc gcctattata tttatttgta tgacaaataa aaccataatc
     4021 cttgcccata cgtccatctg gcttattttt aatcaattca cccgatcttt gatctcatca
     4081 acggtatcaa aataaaccag cgtataacgt tcaaatcgca cataagattc gataaaccat
     4141 ggaggttata tgaaaaaact ggcacttatt ttgtttatgg gaacgcttgt ttccttttat
     4201 gccgatgccg ggcgcaaacc ctgttctggt tcgaaagggg ggatctcaca ctgtacggca
     4261 ggcggcaaat ttgtctgtaa tgatggttct attagtgcat cgaaaaaaac atgcactaac
     4321 tgaagtgtaa aaggggtgcc atgagaaaat gaatctgcta gtcagatgcg cggggaaaat
     4381 ccccgcgctt gcccttacct ggacgtgcag gccgtgagcg cagcaacctc cttatcaccg
     4441 tcccggaacc gcagttcgta gagcgtttgt cgggt
//
LOCUS       DAJTQQ010000193         4467 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000193 DAJTQQ010000000
VERSION     DAJTQQ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.197"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..495
                     /locus_tag="OO309_004047"
     CDS             <1..495
                     /locus_tag="OO309_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5848974.1"
                     /translation="VVIKAGEVIARIHMYKIATLGSGNPRNFTWNIISNNSVVMPTGG
                     CTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLSFYLSGATTDSSRQVFANTAPDATK
                     ASGVGVTLMRNGKILATGENVSLGTVNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVT
                     FIYE"
     gene            829..3108
                     /gene="ydeP"
                     /locus_tag="OO309_004048"
     CDS             829..3108
                     /gene="ydeP"
                     /locus_tag="OO309_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR5848975.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3356..3559
                     /gene="safA"
                     /locus_tag="OO309_004049"
     CDS             3356..3559
                     /gene="safA"
                     /locus_tag="OO309_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR5848976.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3627..4388
                     /gene="ydeO"
                     /locus_tag="OO309_004050"
     CDS             3627..4388
                     /gene="ydeO"
                     /locus_tag="OO309_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR5848977.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
BASE COUNT         1262 a          966 c         1024 g         1215 t
ORIGIN      
        1 gttgtcataa aagcggggga agtcattgcc cgtattcata tgtataaaat tgcaacatta
       61 ggaagcggaa atccgcgtaa ttttacgtgg aatattattt caaataacag tgttgttatg
      121 ccaacaggcg gctgcaccgt tgatagtcgt aatgtcaccg tggatctccc ggactttcct
      181 ggaagcgcag aaatacccct tggcgtatat tgctcaagcg aacaaaagct ctcattttat
      241 ctttcgggtg ccaccactga cagtagccga caagtgtttg ctaacacagc acctgatgca
      301 acaaaagcat cgggagtggg tgtcactcta atgcgtaatg gaaaaatact ggctacggga
      361 gaaaatgtct ccctggggac agtaaataaa agcaaagtac ctctgggttt aagcgcaacc
      421 tatggacaaa caggaaacaa ggtttcagcc ggaaccgtcc agtcagtcat tggtgtcact
      481 tttatctatg aataattgtg attttgacct tagtctatgc gctccattca cgaaaattgc
      541 gcggataatt attttgtgaa ggctattatc ctacacctgt aagaaaatcc gcgcacaatt
      601 gaagccagag tcttattttt tatattggaa aatatgttaa tttaaacact atgagagatg
      661 atgcttaact tatcgtattt aatctattgt ttaacgataa tagattaaaa atagaaagag
      721 agtagtccct tactcttata tatccatgtt ggcgataatc cctttgtatg tactgtgcat
      781 catcgctatt acaaatccta ataattcatt tccacacagg ataagtagat gaagaaaaaa
      841 attgaatcct accagggtgc tgcaggtggt tggggtgctg ttaaatccgt agcgaatgca
      901 gtacgtaagc agatggatat acgccatgat gttattgcca tgtttgacat gaataagcca
      961 gagggctttg actgtccggg ttgtgcatgg ccagatccta agaacagtgc gtcattcgac
     1021 atttgtgaaa acggcgcaaa agcaatcgct tgggaagtca cggataagca ggtaaatgcc
     1081 tctttctttg ctgagaatac ggttcaatca ttacttacct ggggagatca cgagcttgag
     1141 gctgcggggc gtctcactca gcctttgaaa tatgatgccg tcagcgactg ttacaagcca
     1201 ttaagctggc aacaagcttt cgacgaaatt ggcgcacgcc ttcaaagcta tagtgatccc
     1261 aatcaggttg aattctatac ttcgggccgc acttccaatg aagctgcctt tctttatcag
     1321 ctttttgccc gtgaatacgg gagcaataac tttcccgact gctccaacat gtgccatgaa
     1381 ccgacaagcg tgggtttggc agcgagtatc ggtgtaggta aagggaccgt gttgctggaa
     1441 gactttgaga agtgcgattt agtcatttgc attgggcata accctggtac aaaccaccct
     1501 cgcatgctga cttcgttgcg cgctttagtg aaacggggag cgaaaatgat cgccatcaat
     1561 cctctacagg aacgtggcct ggagcgattt accgcaccgc aaaacccgtt tgaaatgctg
     1621 acgaactctg agactcagtt ggccagtgcc tactacaacg tgcgcattgg tggcgatatg
     1681 gcgttgctca aggggatgat gcgcctgtta attgagcgcg atgatgctgc aagtgccgca
     1741 ggtcggccct cattgcttga tgacgaattt attcaaacgc ataccgtcgg ctttgacgag
     1801 ctacgccgtg acgttctcaa ttccgagtgg aaagatatcg aacgtatttc tggactaagt
     1861 cagacacaaa tcgccgaact ggctgacgca tatgccgctg ccgaacgcac cattatctgt
     1921 tacggaatgg ggatcactca gcacgaacat ggtacccaga acgtgcagca actggtcaat
     1981 ttgctgttga tgaaaggtaa cattggcaag cctggtgcgg gtatctgccc actacgtggt
     2041 cactctaatg tacagggcga ccgaaccgtc ggtatcaccg agaaaccgtc tgcagagttt
     2101 ctggctcgcc tgggtgagcg ctatggcttc cccccacctc atgcacctgg acatgctgca
     2161 attgccagca tgcaagcaat atgtacgggg caggctcgag cattgatctg catggggggc
     2221 aactttgcac tggcaatgcc agatcgggaa gcgagcgctg taccgttaac gcaattagat
     2281 ttggcggtac acgtagccac taagcttaac cgctctcatc tgctgaccgc acggcatagc
     2341 tatattctgc cggttctggg gcgtagcgag attgatatgc aaaaaagcgg tgcgcaggcg
     2401 gtaaccgttg aggattcaat gtcgatgatt catgcctcgc gtggcgtgtt aaaacccgcc
     2461 ggtgtaatgc tgaaatcaga gtgtgcagtg gtcgcgggaa tcgcgcaggc agcactaccc
     2521 cagagcgtgg tagcctggga gtatctggtg gaagattatg atcgcattcg caatgacatt
     2581 gaagctgtgc tgccagagtt cgccgactat aaccagcgca tccgtcatcc cggtggtttt
     2641 cacctgataa atgcagctgc tgaaaggcgc tggatgacgc cgtcaggtaa ggctaatttc
     2701 attaccagca aagggctgtt agaagatccc tcttcagcgt ttaacagtaa gctggtcatg
     2761 gcgacagtac gcagccacga tcagtacaac acgacgattt atggtatgga tgatcgctat
     2821 cgaggggtat tcggtcaacg agatgtggtc tttatgagtg ctaaacaagc taaaatttgc
     2881 cgtgtaaaaa acggcgaaag agttaatctt attgcgctta cgccagacgg taagcgcagc
     2941 tcacgccgca tggatagatt aaaagtggtc atttacccta tggctgaccg ctcactggtg
     3001 acctattttc cagaatcgaa tcacatgcta acacttgata accacgatcc attaagtggt
     3061 attcctggct ataaaagtat tccggttgaa ttagaaccat caaattaatg cctcttctca
     3121 tttcttctgc tgtcatccgc acagcagaag aattcctcat tgactattat tgcgcaattt
     3181 gctcacatgg attaaattaa actacatact ataagatata aacttctgcc tacagctgta
     3241 agaaactccg ctcagtactg aagcaccagt cctatttctt cttttctcca gcctgttata
     3301 ttaagcatac tgattaacga cttttaacgt tatccgctaa ataaacatat ttgaaatgca
     3361 tgcgaccaca gtgaaaaaca aaatcacgca aagagataac tataaagaaa tcatgtctgt
     3421 aattgtggtt gtcttattac tgacacttac gttgatagcc attttttcgg caattgatca
     3481 gctgagtatt tcagaaatgg gtcgcattgc aagagatctt acacatttca ttatcaatag
     3541 tttgcaagac tggaaataaa cagcaaatta taaatgtgaa atgtttaaaa ggtatcgata
     3601 aaactttatt gttttaagga gataaaatgt cgctcgtttg ttctgttata tttattcatc
     3661 atgctttcaa cgctaacatt ttagataaag attacgcctt ctccgacggc gagatcctga
     3721 tggtagataa cgctgttcgt acacattttg aaccttatga gcggcatttt aaagaaatcg
     3781 gatttaatga aaataccatt aaaaaatatc tacaatgcac taacatccag acagtgacgg
     3841 tgcctgttcc tgcgaagttt ttacgtgctt caaatgtacc gactggattg cttaatgaaa
     3901 tgattgctta tctcaactcg gaagaacgca atcatcataa tttttcagaa cttttgcttt
     3961 tttcttgcct gtctattttt gccgcatgca aaggtttcat tacactatta actaacggtg
     4021 tgctatccgt ttccgggaaa gtgagaaata ttgtcaacat gaagctggcg cacccatgga
     4081 agctgaaaga tatttgtgac tgcctgtaca tcagtgaaag cctgttgaag aaaaaactaa
     4141 agcaagagca aacgacattc tcacagattc tattagatgc cagaatgcag cacgcaaaaa
     4201 atttgatacg cgtagaaggt tcagtcaata aaattgccga acaatgtggt tatgccagta
     4261 catcttattt tatttatgcg ttccgcaaac atttcggcaa cagtccgaag agagtttcta
     4321 aggagtaccg ttgtcaaagg cacacgggta tgaatacggg caacacgatg agtgctttag
     4381 ctatttgatt atttgctaac gagtagtcaa ccacgcacgc tacgtaagaa ttatatgggg
     4441 cagccattcc ctgccccgcg ttgtttt
//
LOCUS       DAJTQQ010000194         4420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000194 DAJTQQ010000000
VERSION     DAJTQQ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.198"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(216..419)
                     /locus_tag="OO309_004051"
     CDS             complement(216..419)
                     /locus_tag="OO309_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848978.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(421..576)
                     /locus_tag="OO309_004052"
     CDS             complement(421..576)
                     /locus_tag="OO309_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR5848979.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(779..1186)
                     /locus_tag="OO309_004053"
     CDS             complement(779..1186)
                     /locus_tag="OO309_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5848980.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1263..1490
                     /locus_tag="OO309_004054"
     CDS             1263..1490
                     /locus_tag="OO309_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5848981.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1474..1896
                     /locus_tag="OO309_004055"
     CDS             1474..1896
                     /locus_tag="OO309_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848982.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1974..2751
                     /locus_tag="OO309_004056"
                     /pseudo
     CDS             1974..2751
                     /locus_tag="OO309_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2758..3504
                     /locus_tag="OO309_004057"
                     /pseudo
     CDS             2758..3504
                     /locus_tag="OO309_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3519..3941
                     /locus_tag="OO309_004058"
     CDS             3519..3941
                     /locus_tag="OO309_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5848983.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            3999..4355
                     /locus_tag="OO309_004059"
     CDS             3999..4355
                     /locus_tag="OO309_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5848984.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
BASE COUNT         1166 a          957 c         1200 g         1097 t
ORIGIN      
        1 acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc ccccatggag
       61 atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc ggtgatattt
      121 atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat cgcgcgtctg
      181 tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag acccggtgaa
      241 tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc gccagattcc
      301 agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc cagttgctgg
      361 aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt ctcgttcatt
      421 ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt taaacgtttt
      481 gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca cgccgtacac
      541 cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc cccttaacgc
      601 cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat aaaatttaga
      661 ataacctaag gagggcgatc aagatttttg tgtagaaaaa cctaagtttt ttgatgtaaa
      721 aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa aaaggggatt
      781 atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg ttgaaatttt
      841 caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc agtgcgtcga
      901 acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta tccgggatcg
      961 gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag cactgaagcg
     1021 cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt tcccattgag
     1081 aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac agttttaatt
     1141 ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta gggaattcta
     1201 atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct aaggagctcg
     1261 atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt gccaatgccg
     1321 caggagttag gctggcaagc attgctgcat ggggggaact ggttcctgaa ggtcgcgcga
     1381 tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt tatgacgaat
     1441 atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga gcacatcgaa
     1501 tcagtgctgt tagccctggc agccgaaaaa gggcaggcgt gggtcgctaa cgcaattact
     1561 gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa ggactggaac
     1621 aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca aagggaaaaa
     1681 attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg tcaccgactc
     1741 tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc gttgagtacg
     1801 gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca tttcagcggt
     1861 ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcgt gccggattgt
     1921 tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa caaatggctt
     1981 caaactggat aaagctcgag gttattacgc cggataagcc ggaaatattc aggcttgctg
     2041 agattctgaa tattgatcca gatgccgcat tagggaaggt tattcgcttc tgggcatggg
     2101 cggatcaaca aatgatagac ggtaatgcag attgtaacgc tcgcggcgtt acaaaaagtg
     2161 caatagatcg catcactttt atgtctggtt ttgctgatgc gttaattcag gttggatggc
     2221 tggtcgaaaa tgacggtggg ctttctctac ctaactttga acgtcataac ggaaaaagct
     2281 ctaaaaaacg ggcggttaca aacgagcgag taacaaaaat acgcgaactg aaacgaaaag
     2341 gtaacgctgc cagcgttaca caaacggatc aaaaagcgtt accagtggaa gaggaagagg
     2401 aagatctaaa tactgatctc cccctaaatc cccctcgcca aaaacgagcg tctaaaaaat
     2461 tcgagccgga ggctattgag ctgcccgatt ggttgccgga aacactctgg catgagtggg
     2521 tccggttcag acaggcattg cgaaaaccga ttcgaacgga gcagggcgat acgggaactg
     2581 gaaaaattcc gtcagcaggg ttttacacct gagcaggtga ttcgacacag catcgccaat
     2641 gaataccagg gcctgttcgc gccgaaaggt gttcggcctg agacgttgct ccgacaggtt
     2701 aacaccgtct cgttgccgga cagtgcgatc ccgccaggct tcagggggta acggaccatg
     2761 aaaaatattg cgacaggcgg cgttctggaa cgcatccgcc gactgacccc gcaacatgta
     2821 accgccccat tcagaacggt agcggagtgg cgcgagtggc aacttgctga aggccagaaa
     2881 cgtagcgagg agatcaaccg cctgaatcgc cagttgcggg tggaaaaaat tctgaatcgc
     2941 tcaggcatcc agccgttgca ccgtaaatgc tcgtttgcga attaccaggt gcagaacgac
     3001 ggccagcgat acgcgttaag ccaggcgaaa tccatcgccg atgaactgat gaccgggtgt
     3061 acaaattttg cgttcagcgg aaaacctgat accgggtaga atcacttagc ggcagctatc
     3121 gggaatcgcc tgctgaaaga cggtcagaca gtgattgtgg ttaccgtggc tgatgttatg
     3181 agcgccctgc acgccagcta tgacgacggg cagtcaggcg aaaaattttt gcgggagctg
     3241 tgcgaagtgg atctgctggt tcttgatgaa attggcattc agcgcgagac gaaaaacgag
     3301 caggtggtgc tgcaccagat tgttgatcgc cggacagcgt cgatgcgcag cgtggggatg
     3361 ctgacaaacc tgaactatga ggtgatgaaa acattgctcg gcgagcgggt gatggatcgc
     3421 atggtcatga acggcgggcg ctgggttaat tttaactggg agagctggcg tccgaatgtt
     3481 agccattcga gggttgttaa gtagtttcag gaggatttat ggcgaaacct tttactcccg
     3541 aacagcggga agaactgaag acgcgaattg tggaactcgt gcatcaggac ggtcgggtca
     3601 cgattcggca gttgtccgat gaaacaggta tcagtcgtgc gtctgtcggt cgcttatgca
     3661 tagaactggt cgcaagtggt gatgtatata attctggcta cggcttattc ccgtctgaac
     3721 aggctcgcaa ggactggcaa agcgcccgca aaaaactctc gagagtaaag gtgaggaaac
     3781 cggttgttgt tgatccggac cttatctggt cattacctga cggagaaata cgccgctacg
     3841 acaggcgcct gaatataatc tgtcgcgagt gccggaagag cgaagctatg cagcgtgtac
     3901 tggctttcta tcagggtaat tttcaggagg cgatactgtg aatgaaatta gctatcaggc
     3961 ttcaattacc gctggcattc gcatcaaagg agaggagcat ggaaataaaa ccagaagatg
     4021 agttaagcaa tatcgtttta tttccgataa aagaggatga ccctcgtaat caggttaatt
     4081 ttctttataa gccatcggaa agaccatatt gccatcacgc ctctgtacgg gttgacgaaa
     4141 aagagcgtca ggtccgctgt aaaatctgcg gtgcggttgt ggagccgttt gactggatgc
     4201 tctctgtggc aaaaagagaa accagactgg cagatgatgt aaggctattg cgccaggagg
     4261 aacaggaaag gcggagaaat atagaaaagc tgatacagat tgagcgtaac gcgaaagcgc
     4321 ggatacgcag ggcgacaaaa tccagaactg aataattaaa tttagcactg ttaaaaattt
     4381 aatccttaac cggagggatt tctgcaccct caaatcatca
//
LOCUS       DAJTQQ010000195         4319 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000195 DAJTQQ010000000
VERSION     DAJTQQ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.199"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..390)
                     /gene="kptA"
                     /locus_tag="OO309_004060"
     CDS             complement(<1..390)
                     /gene="kptA"
                     /locus_tag="OO309_004060"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR5848985.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHL"
     gene            640..1323
                     /locus_tag="OO309_004061"
     CDS             640..1323
                     /locus_tag="OO309_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCR5848986.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1320..1781
                     /locus_tag="OO309_004062"
     CDS             1320..1781
                     /locus_tag="OO309_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR5848987.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            1794..2966
                     /gene="iadA"
                     /locus_tag="OO309_004063"
     CDS             1794..2966
                     /gene="iadA"
                     /locus_tag="OO309_004063"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5848988.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3031..3942
                     /gene="hypT"
                     /locus_tag="OO309_004064"
                     /gene_synonym="qseD"
     CDS             3031..3942
                     /gene="hypT"
                     /locus_tag="OO309_004064"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5848989.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(3935..>4319)
                     /gene="iraD"
                     /locus_tag="OO309_004065"
     CDS             complement(3935..>4319)
                     /gene="iraD"
                     /locus_tag="OO309_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR5848990.1"
                     /translation="QSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLLVSG
                     MPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIMLYH
                     DDEPLTFDLVWDNGGWRSATLENVS"
BASE COUNT         1001 a         1133 c         1118 g         1067 t
ORIGIN      
        1 gagatgcacg taatgacgat cccctgcaat cagcccctgt ttttttatct cgtcaaggaa
       61 acggcttgcc gtaccgtggt agagaaactg tggcggtgtt ttttcggcaa aggaaatagc
      121 gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac ctaccatcac tggaataact
      181 aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc aatgcacggg ttaatcgctt
      241 gcccgctttt tgggcacaaa gtatgagctt attgatatcc gcccatcctt cacggtccag
      301 tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa cttaaaaact tgctcgtatc
      361 cgccagctct ttttcgttgt actttgccat cgcgttttcg tccctggatt ttctgttttt
      421 atttactaat gtcattgtca ctgggtttat gaattttctg caactcaatc ctgcgcagaa
      481 ccttttccac acagcgtaat ttttccccta tgcaaaaaac gcatagccag aaacatactt
      541 gcattttccc catacccaca tggctgataa ggtttttcag ttccaggaat tttcctgact
      601 caagcaacac acaaacacaa aaaaacaatg gggattgtta tgacacaaca aggggatgct
      661 gtagccgggg agctggcgac agaaaaagtg ggcattaaag ggtatctggc tttttttctc
      721 accataatct tttttcccgg tgttttttcc ggaacagaca gctggtggcg cgtttttgat
      781 ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg cgaatggagc caccacctca
      841 tttcgcgggg tgggtggcgc gggggctaaa gatggctttc tctttgcgct ggagctggca
      901 ccgtcagtca ttctctctct ggggattatt tcaattaccg atggtcttgg cggattacgc
      961 gccgcgcagc agttaatgac acctgtgcta aaaccgttgt tgggcattcc agggatctgc
     1021 tcgctggcac tgatcgctaa cctgcaaaac acggatgccg ccgccggtat gaccaaagag
     1081 ctggcgcagg aaggtgaaat taccgaacgc gataaagtga ttttcgccgc atatcaaacc
     1141 agcggtagcg ccatcatcac caactacttc tcttccggcg tcgcggtgtt tgcctttctg
     1201 ggcacgtccg taatcgtacc tttagccgtc atcctggtgt ttaaatttgt cggagccaac
     1261 attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc caacgcaagg agcgcaagca
     1321 tgacaactca ggtccgcaaa aacgtcatgg atatgtttat cgatggcgcg cgccgtggct
     1381 ttactatcgc cacgaccaac ctgctgccaa acgtggtgat ggcgtttgtc atcattcagg
     1441 cgctgaaaat taccggcctg ctcgactggg tggggcatat ctgtgaaccc gtaatggcgc
     1501 tgtggggatt acccggtgaa gcggcaaccg tccttttagc cgcattaatg agtatgggcg
     1561 gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc attaaccggc cacgatgtca
     1621 ccgttttact gcccgcaatg tatttaatgg gcaacccggt gcaaaatgtc ggtcgctgcc
     1681 tggggacagc cgaagtgaat gccaaatatt acccgcatat cattacggtc tgcgtgatta
     1741 acgcattgct gtcgatctgg gttatgcagc ttattgttta acaaggagtt accatgattg
     1801 attataccgc agccggtttt accctgctgc agggagcgca tttgtatgcg ccggaagatc
     1861 ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat cgccgttgcc agcaatatcc
     1921 cttctgacat tgtaccgaac tgcacggttg tcgatctcag cgggcagatc ctctgcccag
     1981 gttttattga tcaacacgtc catttgattg gcggtggcgg cgaagcaggt cccacgacgc
     2041 gcacgccgga agtggcgcta agtcgcctga cggaagcggg cgtcacgtca gtggttggtc
     2101 tgctgggcac cgactctatc tctcgccacc cggaatccct gctcgccaag acccgtgcgc
     2161 tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc ttatcatgtc ccttcccgca
     2221 ctattacggg ttccgtggaa aaagacgtgg cgattatcga tcgtgtgatt ggcgtgaaat
     2281 gcgccatctc tgatcaccgt tctgccgcac cggacgttta tcacctggct aatatggcgg
     2341 cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg cgtcaccgtg ttccacatgg
     2401 gcgacagtaa aaaggcgtta aagcctattt atgacctgct ggaaaactgc gatgtgccga
     2461 tcagcaagct gctgccgacc cacgttaacc gcaacgtacc gttgtttgag caggcgctgg
     2521 agttcgcgcg caaaggcggc accatcgata tcaccagcag cattgacgaa ccggtcgccc
     2581 ctgccgaagg tattgcccgc gccgttcagg cgggtattcc gctggcacgc gtcaccctca
     2641 gctccgacgg caacggtagc cagccgttct tcgatgacga agggaattta acccatatcg
     2701 gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt gctggtcaaa gactatgatt
     2761 tcagtatcag cgatgccctg cgcccgctca ccagtagcgt agccggtttc cttaacatga
     2821 gcgggaaagg cgaaattctg ccaggcaatg acgcagactt actggtcatg acgccagaat
     2881 tgcgcattga gcaggtatac gctcgcggca aactgatggt caaagacggc aaagcctgcg
     2941 tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg ccctaatgga cgtagaatgc
     3001 ccccaagggc ggctgacaga gtaaaacgta atggatgact gtggtgcggt tttgcataat
     3061 attgaaacca aatggcttta tgattttctg actctggaaa aatgccgcaa tttttcccag
     3121 gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc gccgcatccg tgcactggaa
     3181 caggcgattg gcgttgaatt gtttaaccgc caggtgacgc cactgcaact ctcagaacaa
     3241 gggaaaatct tccattcgca gatccgccat ctgttgcaac agttagaaag caaccttgca
     3301 gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca agatagccgc tgcacactct
     3361 ctttccctcg gtctgttacc gtccattatc agccagatgc cgccgctctt tacctgggca
     3421 attgaagcca ttgatgtcga tgaagcggtc gataaactgc gtgaagggca aagtgactgt
     3481 attttttcct ttcacgacga agatttgctg gaagcgccgt ttgaccacat tcgcttattt
     3541 gaatctcaat tgttccctgt ctgcgccagt gacgaacacg gagaagcact ttttaacctc
     3601 gcgcagccac actttccgtt actgaattac agccgcaact cctacatggt gcgattgatt
     3661 aatcgcaccc tgacgcgcca cagtgagtta agtttcagca ccttttttgt ctcttcgatg
     3721 agcgagcttt taaagcaggt tgccctcgac ggctgtggga ttgcctggct gccggagtat
     3781 gccatccaac aagaaattcg cagcgggcag ctcgttgtgc tgaatcggga cgaacaggtg
     3841 atcccgattc aggcctacgc ataccggatg aatacccgca tgaatccagt tgccgaacgc
     3901 ttctggcgtg aactgcgcga gctggagatt gtgcttagct gacattctca agcgtcgcac
     3961 tgcgccaacc gccattatcc cagaccagat caaacgttag cggttcatcg tcgtggtaca
     4021 gcattatctc aaggctgatc gccagagtgt ggcagtacgc ttctttcaaa ttaacctgca
     4081 acgccgcaat ccgtggctca tgaaataaca gtgcagaacg cagctgacgg caataccagc
     4141 tctggagatg tttgtcgctc gggttatgca cttgccactc gggcatgcct gaaacaagta
     4201 atgacggtaa tgccgaatga ggagaattaa aaagagtgag gagatcgctg cataccgatt
     4261 tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa aacagcctga agtgattgg
//
LOCUS       DAJTQQ010000196         4242 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000196 DAJTQQ010000000
VERSION     DAJTQQ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.200"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            42..2603
                     /gene="mutS"
                     /locus_tag="OO309_004066"
     CDS             42..2603
                     /gene="mutS"
                     /locus_tag="OO309_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR5848991.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2709..3361
                     /gene="pphB"
                     /locus_tag="OO309_004067"
                     /pseudo
     CDS             2709..3361
                     /gene="pphB"
                     /locus_tag="OO309_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3412..4179)
                     /locus_tag="OO309_004068"
     CDS             complement(3412..4179)
                     /locus_tag="OO309_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5848992.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
BASE COUNT         1031 a         1123 c         1104 g          984 t
ORIGIN      
        1 catcacaccc catttaatat cagggaaccg gacataaccc catgagtgca atagaaaatt
       61 tcgacgccca tacgcccatg atgcagcagt atctcaagct gaaagcccag catcccgaga
      121 tcctgctgtt ttaccggatg ggtgattttt atgaactgtt ttatgacgac gcaaaacgcg
      181 cgtcgcaact gctggatatt tcactgacca aacgcggtgc ttcggcggga gagccgatcc
      241 cgatggcggg gattccctac catgcggtgg aaaactatct cgccaaactg gtgaatcagg
      301 gagagtccgt tgccatctgc gaacaaattg gcgatccggc gaccagcaaa ggtccggttg
      361 agcgcaaagt tgtgcgtatc gttacgccag gcaccatcag cgatgaagcc ctgttacagg
      421 agcgtcagga caacctgctg gcggctatct ggcaggacag caaaggtttc ggctacgcga
      481 cgctggatat cagctccggg cgttttcgcc tgagcgaacc ggccgaccgc gaaacgatgg
      541 cggcagagct gcaacgctct aatccggcgg aactgctgta tgcagaagat ttcgccgaga
      601 tgtcgctgat tgaaggccgt cgcggcctgc gccgtcgccc gctgtgggag tttgaaatcg
      661 acaccgcgcg ccagcagttg aatctgcaat ttggcacccg cgatctggtc ggttttggcg
      721 tcgagaacgc gccgcgcgga ctttgtgctg ccggttgtct gttgcagtat gcgaaagata
      781 cccaacgcac gaccctgccg catattcgtt ctatcaccat ggaacgtgag caggacagca
      841 tcattatgga tgccgcgaca cgtcgtaacc tggaaattac tcagaacctg gcgggcggtg
      901 cggaaaatac gctggcttct gtgctcgact gcaccgtcac gccgatgggt agtcgtatgc
      961 tgaaacgctg gctgcatatg ccagtgcgcg atacccgcgt gttgcttgag cgccagcaaa
     1021 ctattggcgc attgcaggat ttcaccgccg agttgcagcc ggtactgcgt caggtcggcg
     1081 acctggaacg tattctggca cgtctggctt tacgaactgc tcgcccacgc gatctggccc
     1141 gtatgcgcca cgctttccag caactgccgg agctgcgtgc gcagttagaa actgtcgata
     1201 gtgcaccggt acaggcgcta cgtgagaaga tgggcgagtt tgccgagctg cgcgatctgc
     1261 tggagcgagc aatcatcgac acaccgccgg tgctggtacg cgacggtggt gttatcgcat
     1321 cgggctataa cgaagagctg gatgagtggc gcgcgctggc tgacggcgcg accgattatc
     1381 tggagcgtct ggaagtccgc gagcgtgaac gtaccggcct ggacacgcta aaagttggct
     1441 ttaatgcggt gcacggctac tacattcaaa tcagccgtgg gcaaagccat ctggcaccca
     1501 tcaactacat gcgtcgccag acgctgaaaa acgccgagcg ctacatcatt ccagagctaa
     1561 aagagtacga agataaagtt ctcacctcaa aaggcaaagc actggcactg gaaaaacagc
     1621 tttatgaaga gctgttcgac ctgctgttgc cgcatctgga agcgttgcaa cagagcgcga
     1681 gcgcgctggc ggaactcgac gtgctggtta acctggcgga acgggcctat accctgaact
     1741 acacctgccc gaccttcatt gataaaccgg gcattcgcat taccgaaggt cgccatccgg
     1801 tagttgaaca agtactgaat gagccattta tcgctaaccc gctgaatctg tcgccgcagc
     1861 gccgtatgtt gattattacc ggcccgaaca tgggcggtaa aagtacctat atgcgccaga
     1921 ccgcactgat tgcgctgatg gcctacatcg gcagctatgt accggcacaa aaagtcgaga
     1981 ttggacctat cgatcgcatc tttacccgcg taggcgcggc agatgacctg gcttccgggc
     2041 gctcaacctt tatggtggag atgactgaaa ccgccaatat tttacataac gccaccgaat
     2101 acagtctggt gttaatggat gagatcgggc gtggaacgtc cacctacgat ggtctgtcgc
     2161 tggcgtgggc gtgcgcagaa aatctggcga ataagattaa ggcattgacg ctgtttgcca
     2221 cccactattt cgagctgacc cagctaccgg agaaaatgga aggcgtcgcc aacgtacatc
     2281 tcgatgcgct ggagcacggc gacactatcg cctttatgca cagcgtgcag gacggcgcgg
     2341 cgagcaaaag ctacggtctg gcggttgctg ctctggcggg cgtaccgaaa gaggttatta
     2401 agcgcgcacg gcaaaaactg cgtgagctgg aaagcatttc gccgaacgcc gccgcgacgc
     2461 aagtggatgg tacgcagatg tctttgctgt cagtgccaga agaaacttcg cctgcggtcg
     2521 aagcactgga aaatctcgac ccggattcac tgactccgcg tcaggcgctg gagtggattt
     2581 atcgcttgaa gagtctggtg taataacaat tcccgatagt cttttgctat cgggaatatt
     2641 aacgacaact gacgaatcaa ataaaaatac cctgtataat aggaaagctt attttacagg
     2701 gtaaaaccat gccatctaca cgctaccaaa aaatcaatgc ccatcactat cgccatatat
     2761 gggtcgttgg tgatattcat ggtgaatatc agttattaca atcccgctta catcaactct
     2821 cttttttccc cgaaaccgac ttacttattt ctgtcggcga taatattgat cgtgggccgg
     2881 agagtcttga cgtcctgcgc ctgctaaacc agccctggtt tatctccgtt aaaggcaacc
     2941 acgaagcaat ggcgctggat gcgttcgcga ccggcgatgg caatatgtgg cttgccagcg
     3001 gtggtgactg gtttttcgat ttaaatgatt gcaacaagag gcaatagttc tgttgctgaa
     3061 attccatcac cttccacata ttattgaaat cactaacgac ataataaaat atgtcatcgc
     3121 acatgcagat tatccgggtg atgaatatct ctttggcaaa gaaatagcgg agagcgaatt
     3181 actctggcct gtttatcgtg tgcagaaatc gcttaatggc gagttacaac aaataaacgg
     3241 cgctgattat tttatatttg gacatatgat gtttgataac attcagacgt tcgctaacca
     3301 gatttatatt gataccggat cgccgaaaag tgggcggctg tcattttata aaataaggta
     3361 gtctcatgct tcttctgtga agcatgagta accagatgtt atttcaggcc attattcatt
     3421 tttcgctacc agcaaagaga gatcctgctt cgccagcgcg cgactggcac tctccggcaa
     3481 accgtcgtct gtaataatct gatcaaactc gcttaatggt aacgccagcc atgtcgccac
     3541 ctgaccatat ttcgtcgcat cacagaccaa aactcgctgg cggctggcac tggcaatcgc
     3601 ccgtttcacc gtgactttat cttccgctgg cgtagaaatc ccccgcacac tccatgacga
     3661 tgcagaaata aaagcctgat caatcatcag gctgcacagc atggtcgcag cggcttcccc
     3721 gacacaggaa cggttttccc gacacactgc accgccagtg tgaataattg tgcaattact
     3781 gttgtcgagc aagtagttcg caataacgaa atcgtttgtg accacagtca gtgactccat
     3841 gtgaatcaga tgctgtgcta tcgctaacgt ggtcgttccc gcatccagat agatacaact
     3901 tcccggcaga acaagacttg ccgccagctt gccaatagcc gctttttgcg tcattgccag
     3961 cgcagttttt acctgatgag aaggttcatg cgccacgcgt cccggagact ggacgcctcc
     4021 ggacaccagc acaacggctc cctgctgctc cagtttttgt aaatcccgac gaatggtcat
     4081 atgtgacaca ttaattctgt ccgttagttc agcaatactg acaatgcctt tttcagctac
     4141 catctcaagg atgatttggc gacgctctac gggtatcaac ttttgctcct tcctttgtcc
     4201 tgctgacatt ctacgctatt tgcctgcgaa acgtgcgcgg cg
//
LOCUS       DAJTQQ010000197         4212 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000197 DAJTQQ010000000
VERSION     DAJTQQ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.201"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            124..1212
                     /locus_tag="OO309_004069"
     CDS             124..1212
                     /locus_tag="OO309_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR5848993.1"
                     /translation="MSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLI
                     FVASLLCWCVGISNYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSF
                     EATFPQALEDVGVTNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRL
                     KMTNNNAHHQCEKWFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQ
                     VRIPKTVRLGILPVIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKY
                     LTQAERLERDQHLLIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRR
                     GDYLTAEKIRKRAARVFVNDERFTRGGN"
     gene            1212..1946
                     /locus_tag="OO309_004070"
     CDS             1212..1946
                     /locus_tag="OO309_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5848994.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            2413..3174
                     /locus_tag="OO309_004071"
     CDS             2413..3174
                     /locus_tag="OO309_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5848995.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3218..>4212
                     /locus_tag="OO309_004072"
     CDS             3218..>4212
                     /locus_tag="OO309_004072"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012786.1,HMM:NF025320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5848996.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSV"
BASE COUNT         1157 a          804 c         1036 g         1215 t
ORIGIN      
        1 attttccagc agcgaaatgt tcttgcgagc tggctggcaa caggttgcgg cgtagcgttg
       61 tatctggctt taacggcgcg tactcgttct ctgacgttga tatgtttgtt gtcgttatac
      121 cctctgtcta cagctttagt ccttacacaa agccgtgtcg gccttcttgg tttattttgc
      181 gtcatcgtgc tggccgctct cgcggacaga ccccgctggc gcggacgtcc ccttagcggt
      241 ctgttgcgct tgatcttcgt cgcctccttg ctgtgctggt gtgtcggtat ctcaaactat
      301 gccatgccag gggagacaac ggctgacttt acgcattcat catcgaatga ccagcgtata
      361 cgtattctga aaggtgccat aagtcttatt caacagcgcc ccttaaccgg ttgggggtta
      421 ggcagctttg aagcgacctt cccgcaagcc ttagaggatg tgggcgtgac aaacatagag
      481 agtgatacgg ctacgcatcc ccataacgaa ctgctgttcg taacggctga aggtggcatc
      541 gtcgccttgt ttggtttttt gctgctatca ggcgtttggc tgtggccgtg tatatgccgc
      601 ctgaaaatga caaacaataa cgcgcaccat caatgtgaaa agtggtttat tgccttaatg
      661 gggttgcctt tagtcattca tatgatgacc gaatatcctc tctatacgtc tgttccccat
      721 ttgatgctgt tgctgctcct tttccgcatc ggaatgccgg aaaacgtgat gaatcaggta
      781 cgtataccta aaacagtccg gttgggtatt ctgcctgtca ttgtgctcat tatgaccgcc
      841 gcgctggtca tcctggtagc gggatttgat gcacagcggg aactgaccag agctgaagct
      901 gatatgaacg aaggatttat ccctcgcctt ccggaacata gctggaaata cctgacgcag
      961 gcagaacgtc ttgagcgcga tcagcatctg cttattgcga ataaaccggg gttcgttcgc
     1021 gatacggaaa gcatgtctct tttcactgtg tggggaaata aatggttggc aatccacaat
     1081 gatgcagacg taagcgctgc aatgatattc attgcgcaca ggcgcggtga ttatctgaca
     1141 gctgaaaaaa taagaaaacg cgcagctcgg gtatttgtaa atgacgaacg ttttactcgc
     1201 gggggaaact aatggttaac gatctgacgt ataagaaatt agaaagcaca aacatcaact
     1261 gtaatattct ctcgccagaa aataccacat ggacaaatgt cacggtgatt ggcctggatc
     1321 tttttgccat ttgcggcgtg tgcagtttgc tcaagcgtgt tgggggagtg aaacttcagg
     1381 ttttcacttc cttacccact gatatgaatg ttctgactga cagtagcatc gtgatatggg
     1441 taaaaatgcg tcacgatggt ttgcctgaat tagccggaca tattgtgaag gtttgtcgtc
     1501 gataccgtct gatgcggcaa ctcgttatta gcgatgctat tcctgaagga atgacgaatg
     1561 atatcgggcc tctttcccga gtatggctta ctcatgggag tgcaaaagtg gatattttat
     1621 tatcttcatt acggaaggtg ttaaatgctc attgtttgcc tggtcctttg tttataaaac
     1681 gcctgggaag agtgcaatgg agggttcttt tactccgcgc gaaggggatt agcacacgta
     1741 aaattgcaga aatctgtggt attggcctca agaccgttag cgcccatgag tccgcaattc
     1801 gagaacgatt aggcaccagg aataaaagtg agtacgtctg gttagtacgt aatgctcaac
     1861 ttatacaaca ggcgataccc gcgttgggaa gagatgcgtc aaggtatgaa agatgtaaca
     1921 atatgcgaac aaagcaaaaa gtataattga tgcgtcatag ataactctct ttttcgatga
     1981 cgaaatcgct gagcatgcgt ggtgaggtta gtaatagatg ggtgctatgt tttgccacgg
     2041 gtttgtagct gtgaaacctg gaaaaaaata agaattaaaa atatttaaag agagttaata
     2101 aggttctggt tgtaattgtc ttggtcaaaa aaaagatatt gtcaatgttg ttttttaaat
     2161 tttcaggttt tggttaaata tttgcttgca aatataggtg attttttatt gtaggctttt
     2221 gaaggctatt ataactgtgc gctaaattaa tgcgttgaat aaaagaaata acaaaattaa
     2281 gaaaagccat atttacgata tgggcaacat aaatataatc taaaaaagag taaggcgttg
     2341 taggttggtt ccgcgttcat taccttgtta gtgaacgcgg atttatcagt tgtcgaaatg
     2401 gtttttataa taatgaaaaa ttttaatgta ctaaccaaag tcgtattttg cttgtcgttt
     2461 ttatcaagcg catttcccac ctatgctgcg gatgatgtca aagtgactca aaacacttca
     2521 gtttttaaac tgaagcttag tgcaacgcga ttaatctatg aacaagggtc ggatggcgca
     2581 gcgataactg tgacaaatcc gcaggattgg ccgatgcttg tgcagagtaa agtctttgaa
     2641 gaagataaag taactgcagc gccctttatt gtcactccgc cactgttcag gcttaatcca
     2701 ggccagcaaa ataaaatgtg ggttgtgcag actgcgccag ctttacaaga cagggaaaaa
     2761 ttacactggc tatgcgccac cggcattacg cctaaaaaga gtgatgtttg ggccgagaaa
     2821 gataaagaaa aaattgctga aaatgtgaac ctgaacatcc aggttaacgt acatacctgc
     2881 ataaaactta ttaccaggcc atctggtatt gaaggcaaga tggttaatgc gagtgagaag
     2941 ttatcgtgga aaaagcaggg tgataaaatt gtcgcggtca atcagtcgcc gttctacatc
     3001 aacctttctt ctttacaggt tggcggtagc aagattgaga aactagatta tattgcgccg
     3061 ttttcgcaac atgagtttgt tatgccaaaa actagcggcc aggatattaa atggtctgtg
     3121 attactgatg aaggtggtga gagtcgtttg tttatatctc atttgcaatc ataatgtatt
     3181 acatttttat tgaatatttt tatgccatgc atattccatg ttttatcgac tgtcattaat
     3241 tttgataatg gccttacttg cgggtcagtt aagtgcccag gagtggtcat ttgattcttc
     3301 acagttggaa ggaaacgtta gtgcggacac tgtggcaatg tttaatcagg gagagcaact
     3361 ccccggaaat tatcgtgtgg aaatttatct taacggtgaa aaagttgatg ttggggagtt
     3421 tccctttcat cgtccggaat cccctgaaga aaaagagctt gttccatgtt taaccgttga
     3481 tgaccttatc cactatggca ttaaaattga taaaagcagc agtgatactg acaataaaaa
     3541 gaatcagtgt tttaaatgga actctattga aggtttgaaa gtaaattatg attttgatag
     3601 ccagcgcgta cagataaccg ttccacaact gtatctgcaa gataaaaaaa gtagtttagc
     3661 accagtgtcg ttatggaatg agggtgtagc tgcattccgc atggtgtatc agacaaatat
     3721 tgatatttcc aaacaaaatg ataatcaatc taccacgcgt aattctcgtt atggcaggtt
     3781 cactccaggg tttaatcttg gtgcctggag gtttcgtagt tcagtgacat ggagcaagga
     3841 acttggccag tcagaaagat ggcaaagggg ttacatgtgg tttgagcgag gcataaatgc
     3901 gattaaaagc cgattaacgc ttggagaatc ttatacgtcg tctgaggtgt ttgacagcat
     3961 accttttcgc ggcgggatgt tagcgaccga cgatgcgatg actccgccgg aggacagcta
     4021 ctacacacct gtggtgcatg gaatagcaca aagtgaggcg caggttatta ttaaacaaaa
     4081 tgggcaaatt atttttaccc gttctgtgcc gccagggcct tttgcgctgg ataatttacc
     4141 aacgctggcc gttggcgggg agcttgatgt tacagtgaga gagagtaatg gcgaggagca
     4201 gtatttttcc gt
//
LOCUS       DAJTQQ010000198         4209 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000198 DAJTQQ010000000
VERSION     DAJTQQ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.202"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO309_004073"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO309_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5848997.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OO309_004074"
     tRNA            complement(324..399)
                     /locus_tag="OO309_004074"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OO309_004075"
     tRNA            complement(406..480)
                     /locus_tag="OO309_004075"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OO309_004076"
     tRNA            complement(597..681)
                     /locus_tag="OO309_004076"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OO309_004077"
     tRNA            complement(690..765)
                     /locus_tag="OO309_004077"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OO309_004078"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OO309_004078"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR5848998.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO309_004079"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO309_004079"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR5848999.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO309_004080"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO309_004080"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR5849000.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1089 a         1046 c          937 g         1137 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggctt
//
LOCUS       DAJTQQ010000199         4163 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000199 DAJTQQ010000000
VERSION     DAJTQQ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.203"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(338..985)
                     /gene="ychE"
                     /locus_tag="OO309_004081"
     CDS             complement(338..985)
                     /gene="ychE"
                     /locus_tag="OO309_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR5849001.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1462..4137
                     /gene="adhE"
                     /locus_tag="OO309_004082"
                     /gene_synonym="adhC"
     CDS             1462..4137
                     /gene="adhE"
                     /locus_tag="OO309_004082"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR5849002.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEDETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1163 a         1013 c          958 g         1029 t
ORIGIN      
        1 aatagagatt ggctaagcat gtctatcttt tctgattaca gttccagtag tgaaatgcac
       61 aataatttaa caattgatta ttatttagca ctttcctcta ccaaaggcag tggaattact
      121 aatatcattt caattattct tcagcaagcc caggattatg atgtggcgaa aataacatga
      181 tggtgaggag aagcagttaa ggaaatcctt ataactaact gtaatttgag aagaatattt
      241 ttacctattc cttttgcttt tgataactgc gcgggaacaa agtaaaaata atgacgaagt
      301 aaacggagca tggcagctcc gtttcatttg aaaggaatta attaagcagg ccggggaaaa
      361 tccccttaat accagtaacg ataaattcaa tccccaatgc catcagcaat agccccataa
      421 tacgcgtaat cacgttgatg ccggtctggc gtaaaacccg taccagccac ggtgccatgc
      481 ggaacaatcc ccagcaacat aaagcgaaca atgcaatagc cacaaagaaa ccaaacagat
      541 agctaatgct gtgataacgc gtaccccaga cgatggtaga actgatcgcc cctggccccg
      601 ccatcaacgg caacgccaat ggcaccacac caatactttc acgtaccgcg gtttctgatt
      661 tttcttgctt gttctgttta tcctcgccaa gcttgccgct gatcatcgac atcgctatcg
      721 tcaccaccag gatgcccccg gcgatacgga acgaatcaat tgatatacca aaaagttgta
      781 gaatcgtgtc gccgagaaaa agcgagatcc acaagataat ggccacagac aggttggctg
      841 taaggttagt tttgtttcgc gctgccgctg tctgataact ggtcatgctg ataaagacgg
      901 gaataatccc taccgggttg accagcgcaa ataacccgat gaaaaatttg aagtaaacgg
      961 gaaaatcaaa aagggtctga atcacggttc gctccgaagc aaaagccgga taatgttagc
     1021 cataaataag gttgaaaaga cgcgctgaca atacgccttt tgacagcatt tttcacctcc
     1081 taactactta aaattgctat cattcgttat tgttatctag ttgtgcaaaa catgctaatg
     1141 tagccaccaa atcatactac aatttattaa ctgttagcta taatggcgaa aagcgatgct
     1201 gaaaggtgtc agctttgcaa aaatttgatt tggatcacgt aatcagtacc cagaagtgag
     1261 taatcttgct tacgccacct ggaagtgacg cattagagat aataactcta atgtttaaac
     1321 tcttttagta aatcacagtg agtgtgagcg cgagtaagct tttgattttc atcggttaag
     1381 caaatcatca ccgcactgac tatactctcg tattcgagca gatgatttac taaaaaagtt
     1441 taacattatc aggagagcat tatggctgtt actaatgtcg ctgaacttaa cgcactcgta
     1501 gagcgtgtaa aaaaagccca gcgtgaatat gccagtttca ctcaagagca agtagacaaa
     1561 atcttccgcg ccgccgctct ggctgctgca gatgctcgaa tcccactcgc gaaaatggcc
     1621 gttgccgaat ccggcatggg tatcgtcgaa gataaagtga tcaaaaacca ctttgcttct
     1681 gaatatatct acaacgccta taaagatgaa aaaacctgtg gtgttctgtc tgaagacgac
     1741 acttttggta ccatcactat cgctgaacca atcggtatta tttgcggtat cgttccgacc
     1801 actaacccga cttcaactgc tatcttcaaa tcgctgatca gcctgaagac ccgtaacgcc
     1861 attatcttct ccccgcaccc gcgtgcaaaa gatgcaacca acaaagcggc tgatatcgtt
     1921 ctgcaggctg ctatcgctgc cggtgctccg aaagatctga tcggctggat cgatcaacct
     1981 tctgttgaac tgtctaacgc actgatgcac cacccagaca tcaacctgat cctcgcgact
     2041 ggtggtccgg gcatggttaa agccgcatac agctccggta aaccagctat cggtgtaggc
     2101 gcgggtaaca ctccagttgt tattgatgaa actgctgata tcaaacgtgc agttgcatct
     2161 gtactgatgt ccaaaacctt cgacaacggc gtaatctgtg cttctgaaca gtctgttgtt
     2221 gttgttgact ctgtttatga cgcagtacgt gaacgtttcg caacccacgg cggctatctg
     2281 ttgcagggta aagagctgaa agctgttcag gatgttatcc tgaaaaatgg tgcgctgaac
     2341 gcggctatcg ttggtcagcc agcctataaa attgctgaac tggcaggctt ctctgtacca
     2401 gaaaacacca agattctgat cggtgaagtg accgttgttg atgaaagcga accgttcgca
     2461 catgaaaaac tgtccccgac tctggcaatg taccgcgcta aagatttcga agacgcggta
     2521 gaaaaagcag agaaactggt tgctatgggc ggtatcggtc atacctcttg cctgtacact
     2581 gaccaggata accaaccggc tcgcgtttct tacttcggtc agaaaatgaa aacagctcgt
     2641 atcctgatta acaccccggc ttctcagggt ggtatcggtg acctgtataa cttcaaactc
     2701 gcaccttccc tgactctggg ttgtggttcc tggggtggta actccatctc tgaaaacgtt
     2761 ggtccgaaac acctgatcaa caagaaaacc gttgctaagc gagctgaaaa catgttgtgg
     2821 cacaaacttc cgaaatctat ctacttccgc cgtggctccc tgccaatcgc gctggatgaa
     2881 gtgattactg atggccacaa acgtgcgctc atcgtgactg accgcttcct gttcaacaat
     2941 ggttatgctg atcagatcac ttccgtactg aaagcagcag gcgttgaaac tgaagtcttc
     3001 ttcgaagtag aagcggaccc gaccctgagc atcgttcgta aaggtgcaga actggcaaac
     3061 tccttcaaac cagacgtgat tatcgcgctg ggtggtggtt ccccgatgga cgccgcgaag
     3121 atcatgtggg ttatgtacga acatccggaa actcacttcg aagagctggc gctgcgcttt
     3181 atggatatcc gtaaacgtat ctacaagttc ccgaaaatgg gcgtgaaagc gaaaatgatc
     3241 gctgtcacca ccacttctgg tacaggttct gaagtcactc cgtttgcggt tgtaactgac
     3301 gacgctactg gtcagaaata cccgctggcg gactatgcgc tgaccccgga tatggcgatt
     3361 gtcgacgcca acctggttat ggacatgccg aagtccctgt gtgctttcgg tggtctggac
     3421 gcagtaactc acgccatgga agcttatgtt tctgtactgg catctgagtt ctctgatggt
     3481 caggctctgc aggcactgaa actgctgaaa gaatatctgc cagcgtccta ccacgaaggg
     3541 tctaaaaatc cggtagcgcg tgaacgtgtt cacagtgcag caactatcgc gggtatcgca
     3601 tttgcgaacg ccttcctggg tgtatgtcac tcaatggcgc acaaactggg ttcccagttc
     3661 catattccgc acggtctggc aaacgccctg ctgatttgta acgttattcg ctacaatgcg
     3721 aacgacaacc cgaccaagca gactgcattc agccagtatg accgtccgca ggctcgccgt
     3781 cgttatgctg aaattgccga ccacctgggt ctgagcgcac cgggcgaccg tactgctgct
     3841 aagatcgaga aactgctggc atggctggaa acgctgaaag ctgaactggg tattccgaaa
     3901 tctatccgtg aagctggcgt tcaggaagca gacttcctgg cgaacgtgga taaactgtct
     3961 gaagatgcat tcgatgacca gtgcaccggc gctaacccgc gttacccgct gatctccgag
     4021 ctgaaacaga ttctgctgga tacctactac ggtcgtgatt atgtagaaga tgaaactgca
     4081 gcgaaaaaag aagccgctcc ggctaaagct gagaaaaaag cgaaaaaatc cgcttaatca
     4141 gtagcgctgt ctggcaatat aaa
//
LOCUS       DAJTQQ010000200         4042 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000200 DAJTQQ010000000
VERSION     DAJTQQ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.204"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(886..1077)
                     /locus_tag="OO309_004083"
     CDS             complement(886..1077)
                     /locus_tag="OO309_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5849003.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENL"
     gene            1142..1465
                     /locus_tag="OO309_004084"
     CDS             1142..1465
                     /locus_tag="OO309_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR5849004.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1407..2165
                     /locus_tag="OO309_004085"
                     /pseudo
     CDS             1407..2165
                     /locus_tag="OO309_004085"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2143..2673
                     /locus_tag="OO309_004086"
     CDS             2143..2673
                     /locus_tag="OO309_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR5849005.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2680..2913
                     /locus_tag="OO309_004087"
     CDS             2680..2913
                     /locus_tag="OO309_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR5849006.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3459..3851)
                     /locus_tag="OO309_004088"
     CDS             complement(3459..3851)
                     /locus_tag="OO309_004088"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849007.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          966 a          917 c         1207 g          952 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacaa gcccgtcaga ggttttcacc
      901 gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt cgtaaagcga
      961 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca gcagcgttaa
     1021 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     1081 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgcgagag
     1141 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg
     1201 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
     1261 gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg ggaataacct
     1321 gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     1381 ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga
     1441 acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     1501 cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta
     1561 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg
     1621 gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa gtgatggcga
     1681 gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt ctgtgggtgg
     1741 agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg gagctacaga
     1801 ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc
     1861 agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag aaccgccgga
     1921 cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag agcattacac
     1981 ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg
     2041 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     2101 acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa tcttttacag
     2161 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     2221 agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc aaacgagacg
     2281 aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact gaaaaaacat
     2341 cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg
     2401 aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag tggcgggacg
     2461 ctctggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa gcagaacgag
     2521 agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg gagcaacgga
     2581 cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat ggaaaacggg
     2641 aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct ggaaagccat
     2701 ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa cgaatgggag
     2761 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
     2821 aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca gacagaacgc
     2881 ttgagccagt tatacagcga gaactggaga taagagagga acggttgata caggagcgcg
     2941 aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg acactggatg
     3001 gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc
     3061 attttttggg ataatggttg gttcgggggc accgggaacg cgcttcagtc agaccttgct
     3121 gaaaacacag gaacgagtta aaacgcatac gggcgcgatt ctggtgtgtc ttttgtcgct
     3181 gtgttttgcc tctattgcca gatcgcgctc attcccctga acttctgttt tcccgctcag
     3241 gtccccgtct tcttcgcttc cgaaagcatg aataaaatcc gtttcagctg tctctggctg
     3301 gcaattcctg cgtgattcac atggctgcat agctatgcat gtggttaaaa tttaccgggt
     3361 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     3421 gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt acgttaaatt gtgagggaat
     3481 ggtccccata tactgacttt atcataggga tagggttggt agtgggtcga ttgggtttcg
     3541 gcattgtttc cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat
     3601 tctcctgggc ctggaaaggc taagtgttta gaattgatgg caaaatatct tccgtccgat
     3661 ttttgaagta gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt
     3721 tgtagttttt gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt
     3781 attattctct cttttaataa gctgtttacc acagggttta gttgttgtct aaagcacgca
     3841 gataatgcca ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt
     3901 gtttgatgtg atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg
     3961 gctataaaac aaaattttca tagagtgatt caagatcttc ttgagatcct tttttgcggc
     4021 gcgtaatctg ctgcctgcaa ac
//
LOCUS       DAJTQQ010000201         3974 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000201 DAJTQQ010000000
VERSION     DAJTQQ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.205"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(119..2152)
                     /gene="metG"
                     /locus_tag="OO309_004089"
     CDS             complement(119..2152)
                     /gene="metG"
                     /locus_tag="OO309_004089"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR5849008.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2284..3393
                     /gene="apbC"
                     /locus_tag="OO309_004090"
     CDS             2284..3393
                     /gene="apbC"
                     /locus_tag="OO309_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR5849009.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3655..3936
                     /locus_tag="OO309_004091"
     CDS             3655..3936
                     /locus_tag="OO309_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR5849010.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT          976 a          981 c         1033 g          984 t
ORIGIN      
        1 cagttgaatg cagatgctac cagtatttat acgggttaga gagagacaaa atcgcaagaa
       61 aacaacttta ggaatttata aagaaaaggc gctgtcgatg cagcgccttg aagggggatt
      121 atttcacctg atgacccggt ttagcaccgg catccgggct tagcaggaaa atatctttcc
      181 cgccaggacc ggcagccatc accatgcctt cagagatacc gaagcgcatt ttacgtggtg
      241 ccaggttagc caccataatg gtgtgacgac caatcagtgc ctgcggatcc gggtaagcag
      301 aacgaatacc ggagaagaca ttgcgttttt caccgccgag atccagcgtc aggcgcagca
      361 gtttgtcaga accttcaaca aactctgcgt tttcgatcag cgccacgcgc aggtcaactt
      421 tggcgaagtc gtcaaaggtg atggtttcct gaatcggatc atctgccagt gggccagtaa
      481 ctggcgcggt agtggctttc acttcttctt tagaggcttc caccagagct tcaacctgtt
      541 tcatatcgat gcggttatac agcgccttga acggattcac tttgtggccc agcagcggtt
      601 gctggatacc atcccaggtc agttccgtat tgaggaaggc ttctgcacgc tcggtcagtt
      661 tcggcagtac aggcttcagg taagtcatca gtacgcggaa caggttgatg cccattgagc
      721 aaatcgcctg caggtcagca tcgcgacctt cctgttttgc gactacccac ggagcctgct
      781 cgtcgacata gcggttagcc aggtcagcca gtgccatgat ttcgcgcact gctttaccaa
      841 attcacggct ttcccacgct tcaccaatca cttcagcggc atcggtaaag gttttgtaca
      901 gctcagggtc agccagctcg cttgccagca caccgtcaaa acgcttgttg ataaagcccg
      961 cattacggga ggccaggtta accactttgt tcacgatatc ggcattcaca cgctgaacga
     1021 aatcttccag gttgagatcg atatcatcaa tgcgcgaaga gagtttcgca gtgtagtagt
     1081 aacgcaggct gtcagcgtca aaatgattca gccaggtgct ggctttaata aaggtgccgc
     1141 gagacttgga catcttcgcg ccgttcaccg tcacatagcc atgaacaaac aggttggtcg
     1201 gcttgcggaa gttgctgcct tccagcatgg caggccagaa caggctgtgg aagtaaacga
     1261 tatctttacc gatgaagtgg tacagctcgg cggtggagtc tttcttccag tattcatcga
     1321 agcttacgct gtcgccgcgc ttgtcgcaca gattcttgaa agaacccatg tagccaatcg
     1381 gtgcgtccag ccagacgtag aaatatttgc ccggtgcgtt cggaatttca aaaccaaagt
     1441 atggcgcgtc gcgggagata tcccactgtt gcaggccgga ttcaaaccac tcctgcattt
     1501 tgtttgccac ctgctcctgc aacgcaccgc tgcgggtcca tgcctgcagc atttcgctga
     1561 aagagggcag atcaaagaag aagtgttcag aatcacgcat cactggtgta gcaccagaaa
     1621 ccaccgattt cggctcgatc aattcagtcg ggctgtaggt cgcgccgcag acttcgcagt
     1681 tatcgccgta ttgatccggg gatttacatt tcgggcaggt gcctttcaca aaacggtccg
     1741 gcaggaacat gcctttttcc ggatcgtaca gctgagagat ggtgcggttt ttaataaaac
     1801 cgttttcttt caggcgagag tagataagtt ctgacaactg gcggttctct tcgctgtgcg
     1861 tcgagtgata gttgtcatag ctgatgttaa agcctgcgaa atcagtctga tgctcctgac
     1921 tcatttcgcc aatcatctgc tccggggtga taccgagctg ctgagctttc agcatgatcg
     1981 gtgtaccgtg ggcatcgtcg gcgcagatga agttgacctc gtggccgcgc attcgctggt
     2041 aacggaccca gacatcagcc tggatgtgct ccagcatatg gccgaggtgg attgagccgt
     2101 tagcgtacgg cagtgcgcac gtcaccagaa ttttcttcgc gacttgagtc atagtaggca
     2161 ttacttctta atagtgaaaa ggggttagat agtaccaaat gggaaaatgt taagtaagta
     2221 cttctctata ttcttcaggt aaactatcag ctgatggttt atctatacaa aaaaggagtc
     2281 gggatgaacg aacaatccca ggccaaatca ccagaagcct tgcgcgcaat ggtcgccggg
     2341 acactggcca attttcagca cccaaccctg aagcataacc tcaccacgct gaaagcgttg
     2401 caccatgtcg cctggatgga cgacacgttg catgtggaac tggttatgcc gtttgtctgg
     2461 catagcgcgt tcgaagagtt aaaagagcaa tgtagcgccg aactgctgcg catcaccggg
     2521 gcgaaagcta tcgactggaa actgtcgcat aacatagcca ctctgaaacg cgtgaagaat
     2581 cagccgggaa ttaacggtgt gaagaacatt atcgcggtga gttccggtaa aggcggcgtg
     2641 ggtaaatcgt ccacggcggt aaacctggcg ctggcactgg cggcggaagg ggcgaaagtc
     2701 ggcattctgg acgccgatat ctacggccca tcaattccca ccatgctggg cgcagaaaat
     2761 cagcgtccaa cttcgccgga cggtacccac atggcaccga ttatgtctca tggcctggca
     2821 accaattcaa ttggttatct ggtcactgac gacaatgcga tggtgtggcg tggaccgatg
     2881 gcgggcaagg cgttaatgca gatgttgcag gaaaccttgt ggccggatct cgactatctg
     2941 gtgcttgata tgccgccagg caccggtgac attcaattga cgctggcgca aaacattcca
     3001 gtaactggtg cagtcgtggt caccaccccg caggacatcg cgttgatcga tgcgaaaaaa
     3061 ggcattgtga tgttcgagaa agtcgaggtt ccggtgctcg gtattgtcga gaacatgagt
     3121 gtgcatattt gcagtaactg tggtcaccat gaaccgattt tcggtaccgg tggtgcagag
     3181 aaactggctg aaaaatacca tacacagctg ctggggcaga tgccgttaca catctcctta
     3241 cgtgaggatc tcgataaagg cacgccaacg gttatcagcc gtccggagag cgaatttacc
     3301 gctatctacc gccagctggc tgaccgcgtt gcagctcagc tctactggca gggtgaagtc
     3361 attcctggcg agatttcctt ccgcgcggtc taacgcctat taaccattct ggttattctt
     3421 catacaccgc agagatgtta tattgatgcg gtgtatttgt ttttgccgca ttaatgaaca
     3481 cttatttaat actgcgaata ttctgcggac cttcgtccga aactctacaa agtcgagtga
     3541 tcgtacaggc aatgcttcat tatcatcctt cgtgctgtgt agtggatgta cagctatagt
     3601 ttgttatgca ctcattctat ccaggcataa cctttactac agggataacg ttcaatgaat
     3661 aagtattggc tgtcaggaat tatttttctt gcctatggat tggcttctcc cgcagtctct
     3721 tcagagactg ccacattaac gattaatggc aggatcagtc ccccaacatg cagtatggct
     3781 atggttaata gtcaaccaca acagcattgt ggtcagttaa cgtataatgt tgacacccgt
     3841 catcaggttt cttcgcccgt caaaggtgtt acgacagaag tggttgttgc aggtagcgat
     3901 agcaaacgtc gaatcgtgtt aaatcgctac gattaatttg gcaacctgat tttaattgca
     3961 tttagaaaga cgaa
//
LOCUS       DAJTQQ010000202         3899 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000202 DAJTQQ010000000
VERSION     DAJTQQ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.206"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..360
                     /locus_tag="OO309_004092"
     CDS             <1..360
                     /locus_tag="OO309_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5849011.1"
                     /translation="PPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            361..726
                     /locus_tag="OO309_004093"
     CDS             361..726
                     /locus_tag="OO309_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5849012.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            723..1406
                     /locus_tag="OO309_004094"
     CDS             723..1406
                     /locus_tag="OO309_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5849013.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
     gene            1605..1680
                     /locus_tag="OO309_004095"
     tRNA            1605..1680
                     /locus_tag="OO309_004095"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1638..1640,aa:Ile,seq:cat)
     gene            1782..1858
                     /locus_tag="OO309_004096"
     tRNA            1782..1858
                     /locus_tag="OO309_004096"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1816..1818,aa:Arg,seq:tct)
     gene            2381..2489
                     /locus_tag="OO309_004097"
                     /pseudo
     CDS             2381..2489
                     /locus_tag="OO309_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2770..3105)
                     /gene="iraM"
                     /locus_tag="OO309_004098"
     CDS             complement(2770..3105)
                     /gene="iraM"
                     /locus_tag="OO309_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR5849014.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            3365..3553
                     /locus_tag="OO309_004099"
     CDS             3365..3553
                     /locus_tag="OO309_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR5849015.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            3550..3711
                     /locus_tag="OO309_004100"
     CDS             3550..3711
                     /locus_tag="OO309_004100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849016.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
BASE COUNT         1044 a          739 c         1024 g         1092 t
ORIGIN      
        1 ccaccacagg aagagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat
       61 ctccagcgcc agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg
      121 cagaaatgca tgacgtgtgg caatcaggca gatgatccgc accacatcat tggtcatgga
      181 ctgggaggga tgggaacaaa ggctgacgat ttgtttgtta ttccgctgtg tcgtaaatgc
      241 cataacgaac tacacgccgg ggtaaaagat tttgaagaaa aacacggtag tcagttgttg
      301 ttgctgattc gttttttaat gcacgcgaga aattcgggtg ttctgaagtg gaaagcataa
      361 atgaccgaac gcatagaatt tgttttgcct tacccgccaa cggtgaacac ctactggcga
      421 cgtcgtggca gcacatattt tgtatcaaaa gccggtgagc gttatcgccg tgatgtggcg
      481 ctaattgttc gccagcagcg actgaaatta aacctgtccg gaaggctggc gataaagatt
      541 attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa ggcaccgctg
      601 gatgcgctga cgcatgctgg actacttata gacgacgagc agtttgatga aatcaatatt
      661 gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa aaatcacaga actggggtgc
      721 gcatgaataa ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt
      781 tgagtggggc aacaaaaggt cagcttgaag cctggcaaga gaatgccatg ttcgatacag
      841 ggcgttacag gcgtaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      901 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg
      961 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagagc
     1021 actataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
     1081 cgataacaca gtgggcgtgg aatgaattta atgctcaatc cggtaccaga aaaattgcag
     1141 ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag
     1201 aactttttgg tggggaaggt tatgaatacc aggagctggc attactggcg ggagtgacaa
     1261 ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc
     1321 gactggatag tgaggcttta ttgtttgtaa tgagaacacg ttcaaaaaaa agacggcatt
     1381 ttcaaagcaa agtgttgcaa aagtagattg aaaggcatat attcatgcaa atctgatact
     1441 ttgccgattt tgtacgtgat ggcaaaagca aacaaaaccc gcccacaagc gggttttttg
     1501 tgccacttat ctcggataga catggtgaat gcgctggtgg aggagataag ggtgattttt
     1561 gaatgcttgc aacattgatt tcgtaacgtt attatcctgc gcccggccct ttagctcagt
     1621 ggtgagagcg agcgactcat aatcgccagg tcgctggttc aaatccagca agggccacca
     1681 tcacaaaccg ccattagctt atcaggaaga gcagacgaca cgataacagg gttgttggtg
     1741 cggggggcgg gtccccgatg gcggtccatt atcggtattc agcgttgtta gctcagccgg
     1801 acagagcaat tgccttctaa gcaatcggtc actggttcga atccagtaca gcgcgccata
     1861 ttcattcttc cagattcctt ccggcagagc cttatactga aatatacctg gctcaggata
     1921 ttgttgaaaa tattatatat ttgtcaaaaa taaaagttct gttaagtatt gattgagtat
     1981 ttgttatacg gtctaatggt tttttcagca ttaagtattt atcattcata tggtgtgggt
     2041 agagtgaata ttgatgaggc gtcggggtgt ttcatcctta ggcagcgtat tgatatagtc
     2101 aatgcagcac gagcaaaggc cttcagccgt ttgacagttt tgttctgtac tcctgatcgt
     2161 ctttcgggaa gagacgttat tattctgaat agtgatgcta tacagagggt ttgcgatgag
     2221 ttcatggttg ctaattcaga attatttgct cttgttcagg agtacaacag aatagccagg
     2281 acctgtggta tggatgaact tcggattact catctggggt agatacatat ctggattatc
     2341 acctgttacg gtaaaaagtg attgcttact gtttttgtta atggcattgc agcagccgga
     2401 taatgtcagt gccggctgac ggtgtgctgg tggcgggtgt ggtggttgtt gctttcccgt
     2461 tgctgaaaaa gaaaacgcca gactgttagc cgggtatcag ttagcgggag aaatttttaa
     2521 atacttcaca attcaggcgg ttgactgttg tctggtttgc ggggagtttg ttaaaagaaa
     2581 ctggcatggt gaatccccct gtgcggaggg gcaatcagcg agtaggtata tgggataatc
     2641 gcggattcag gtgctggtac tgaattcacc gggaggcacc cggcaccatg gaatggcaca
     2701 tagcgccact ctccagcccc tctccggagg ggctgtttat attgattttg tcagatgtga
     2761 gtaaactcct tatggacttt gttgttttag tccataaggg catatttgca gagtgcaacg
     2821 gttattaaag cattcattca atacgttatc tgtatttgta gggcattcct ggctgttttt
     2881 gattaaattc cagaatgttt tattgaatgg tactacgttg taaatggtta caggtagcac
     2941 tttgttattg agcatgatgc ctgtgtgagt cagtgtaaat atactttcag gaggtaagaa
     3001 agcatccgat tgataccaga ttattaattt tattttactc catatgactg aaaaagatat
     3061 tccgcatgat ggctggataa ctgtatcaat cacaatccac ttcatttagt ttccttgttt
     3121 atgccttgct ggtgatgttc tgaaaagtat aaatgatatt tttgaattaa accatagaac
     3181 agaattattt ttctgatgtt gtttattgtt tatttaaatg cagggtggtt tatatctcgt
     3241 cttgtagttt atccatgcat atctgcttga taatcaggtt tttatttaag gtatggtttt
     3301 gtgttttttc tgtattacat gtcaggtatt ttaaagaatt atttttcaga tgttggaaag
     3361 aaccatggca tttaaacact atgatgttgt cagggcggcg ccgccgtcag atcttgcgga
     3421 aaagctgaca cataaactga aagagggctg gcagccgttt ggtagtccgg tggccataac
     3481 cccttatacc ctgatgcagg cgattgcagc agaaggtgat gtggtggtca gtggtgcaac
     3541 tgagccggag tgatactacg tcatcgtact ggcccggcat tccaggccat aaaagacagt
     3601 ctggcagtgg gactaaatgc actgacgctg acggatatta ccaaaaatgc aacgtatggc
     3661 gttgagatag aaagtctggt gctggagata aatgcaccgg catcatcata aaaagtgagc
     3721 cagtcaaatg gaaggtatcg ttaaactcac cggtagtgtc agtgggtcgt ctgagacgct
     3781 tgcatgagtt atcagagcca tcagtagtta actggtggct tttttattgt tgtcagcttc
     3841 cggataacgg gagacggggt atggaccaga tggaaaaaat cacaacaggt gtgtcatac
//
LOCUS       DAJTQQ010000203         3849 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000203 DAJTQQ010000000
VERSION     DAJTQQ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.207"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..401
                     /locus_tag="OO309_004101"
     CDS             <1..401
                     /locus_tag="OO309_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849017.1"
                     /translation="QTSPDIKNREIRLEWSGDKRDLPTAEEEISRVQASIIKWYASEY
                     HNGRQVLDEIQTPSAINSELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICL
                     ALGFFLSIVISVMFCLVKKMVDEYQQNSGQ"
     gene            complement(644..1339)
                     /locus_tag="OO309_004102"
     CDS             complement(644..1339)
                     /locus_tag="OO309_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR5849018.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1332..2759)
                     /locus_tag="OO309_004103"
     CDS             complement(1332..2759)
                     /locus_tag="OO309_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR5849019.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2770..3489)
                     /locus_tag="OO309_004104"
     CDS             complement(2770..3489)
                     /locus_tag="OO309_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR5849020.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1003 a         1029 c          812 g         1005 t
ORIGIN      
        1 ttcagaccag cccagatatc aagaacagag aaataaggct ggaatggtca ggagataaga
       61 gagatttacc aactgctgaa gaggaaatat ctcgcgtgca ggcgtctatt atcaaatggt
      121 atgcgtcaga ataccataat ggcaggcaag ttctcgatga gatacaaacg ccttcagcaa
      181 ttaacagtga gctttataca aaaatgatat acctgaccag gaactggtca ttgtatccga
      241 acggtgatgg ctgtgtaact ataagctcac cagaaataaa aaataaatac cctgctgcca
      301 tttgcctggc tctgggattt tttctaagca ttgtaatttc tgtaatgttt tgccttgtca
      361 aaaaaatggt agatgaatac caacaaaact ctgggcagta ataaacagca ttctgataga
      421 gagtgcctgt ataaagctga agtgacaatg accgggaagg gaacccgttt tcaggaatgg
      481 atgcctgtct ttttcactgt aaatatcctg gcattactaa tgcctggata tttacagaag
      541 agcagcataa ccatgacgga acggggttaa gtcaggttgt ccttaacgct attttcaacc
      601 tcgtatgcct tcttcaggtt tatgttcaga tctcatatct ctctcagcaa tcctcaataa
      661 tcagatacac cgctttcacc ggaccatgaa ccccgacgac tttgataagc tcaatatccg
      721 ccgttgagct gggaccactg atgatgttaa tacatgacgg cattcgttca ccggcctgcg
      781 ctttctgatg caatttttct gcgagttgcg ctacacgcgg caggattgtg cttttacgca
      841 ggataaaaag agaagattcc gggagcaggc tcaatgaacg cccgcgctcg gcggcggaaa
      901 aaagaaccac gcctcccgat tcggttaaac catattcagc atacacgaca cccactttag
      961 cctgctctgc ctgcgagata ttctcggcac ctttcgccgg atcccaaaca acggcattgc
     1021 attcctgctg caaacgttca ctaatcccca attcctccag cctcgtgtca ccgctaatca
     1081 cgaccgactg atctcccagc tctttacaca gacgtattgc agcttctgcc gccttcgcct
     1141 cgctggtcag ctcacagcgc gtcaacataa catcgctggc aaactgaata aacgcgtcac
     1201 agcgctgctg ttggttaagt tgggtaagcc gctcgttagc atagttgtta agcggcgcat
     1261 cttctgcttg cggttcaagt cgcagcgggc gacccagtgc ctgagcaacg ttattcaaaa
     1321 attcgctccg attatccatt ctttttctcc tgcgcctgat gtttcttaaa ccaactacgg
     1381 aaactctctc cgtcagcttc aggaagatcg cgtgcttcca tccagtcgct aatcgcgcca
     1441 aatttgagtg gtgttttgcc gccattgata aaccagcttg ccgcatgagc gccggccatc
     1501 atcccgactt tccacaatcc tggatgacta ttggcataag cgaacatttt tatcgcccgt
     1561 tgctctgctt ttgcggtgat ccctttttca gccatcaccc gacgatgacg caaaatcagt
     1621 tttgacagcg gaatacgcac cggacacacg ctgtcacaag cagtgcataa agagcaggcg
     1681 tagggtaaat ctttaaaatc tttatagccg ccaagtagcg gagaaatcac cgcaccaatt
     1741 ggccctggat aaatagagcc atatccatga ccgccaatat ggcgatatgc cggacaagta
     1801 ttcatacaag ccccgcagcg aatacagcgc agcacatccc gaaattcaga ggccagcacc
     1861 tcagaacgcc cgttatcgac aataaccaga tgaaactctt caggaccatc aacgtgcccc
     1921 gcttcgcgcg gccctgtcag ccaggtgttg tatcccgtca aacgtgcacc aacggcactg
     1981 cgcgccagca tggtgatcaa tacatctacc tcggcaaacg tgggggcaat acgctccatt
     2041 cccatcactg caatatgcgt tttaggcagc gtggtacaca ttcgcgcatt accttcattg
     2101 gtcaccaggc ataccgaacc ggtctctgcc accgcgaaat tacagccggt aatacctatt
     2161 tcagcactga ggaaatcttc acggattttt tgccggatga ataaggtcat cgcttcaggc
     2221 gtttccggcc cctcatagcc cagacgttcg tgtagcactc gacggatctg atggcgatct
     2281 ttatgaattg ccgggaccac aacatgagat ggcggatctt gatccagctg gagaatatat
     2341 tcacccagat cggtttcaat cacctgaatg ccagcatcct gcaacacatg attgacacca
     2401 atctcttcgg tcaccatcga tttagatttc accaccttcc gggcattttt gcgttgggca
     2461 acctgtaaaa tgtagcgggt agcgtcttct ttggtttttg caaaatagac gtgaccgccg
     2521 ttttgcgtca ctttttctga gagctggtac agataagcgt cgagattact cagaacatga
     2581 tcacgtatct gggcggcccg atcgcgccac tcctcccagt gccccaattc atcgaccatt
     2641 ttttgccgat ttgccccaat acgctgctgc gcgcttgcca ccgctttgcg catgatcgga
     2701 tcttcaattt gctgacggat gcgtgtctta aaatctgtat tactggtttt gatcgacatc
     2761 tttatatcct cagcggctca tcaacacttc agcaatatgc atcactttga ctttctgccc
     2821 ttcccgttgt aatcgcccac tgatgttcag caggcaactc acgtcagcac caattaaata
     2881 ctcagggcgg acttccatca ggtgcacaac cttttctttc accatctcgc cggatatttc
     2941 ggccattttg accgagaacg tgccgccaaa tccgcagcag gtatcctgtt cagcaaaggt
     3001 caacagctcc agtccacgca catttttcag cagcgtaagt ggctcgtcct tcactcccag
     3061 cttacgggcc aggctacaag atgggtgata cacagctctc ccttgcaaac tggcacctac
     3121 atcgactacc cctaatttat taacaataaa agaggtgaga tcctgcatac gcgcggcaac
     3181 cttttcggca cgtgatgccc attcaggttc atccgccaga tacgtcgggt aactttttat
     3241 ggcataggtg caagagccag ccggtgaaat aatgggatcg tcgttatcct ccagtgcggc
     3301 gatcagattt ttcatccctg gaatcgcttc tttgatataa ccgctattga tcgcaggctg
     3361 accgcagcat ccctgtttct ccgggaaatt tacgcgacag ccgagttttt ccagtagcag
     3421 cacggagtct cgtgccattc ttgatttcag ggcgtcacca atacaggtga caaagaaatt
     3481 gacattcaca actaatactc cattacttca tgcccattta tgcccgatgt ttccccccca
     3541 ccgggggatc aatcatcaat aaatatggcg ttctttttta attggctagc aacataccac
     3601 ccctcatttg tatgacaaat gataatcttt catccaaact tcaaatgcaa taactaattt
     3661 tatttctttc ggcatcgctc acattttatg gggaaatcga cgatcatttt tggcataaga
     3721 aatccattta aaaatggcta catcccttat acctattcag agctacttat tcaggggtta
     3781 ttgagatata cactgatggt gtacaaaaaa atcaccgaca ataatcacgt atcaagtaat
     3841 agtcggaat
//
LOCUS       DAJTQQ010000204         3806 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000204 DAJTQQ010000000
VERSION     DAJTQQ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.208"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            437..2089
                     /locus_tag="OO309_004105"
     CDS             437..2089
                     /locus_tag="OO309_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5849021.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2100..2270)
                     /locus_tag="OO309_004106"
                     /pseudo
     CDS             complement(2100..2270)
                     /locus_tag="OO309_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2403..2522)
                     /locus_tag="OO309_004107"
                     /pseudo
     CDS             complement(2403..2522)
                     /locus_tag="OO309_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2596..3526)
                     /locus_tag="OO309_004108"
                     /pseudo
     CDS             complement(2596..3526)
                     /locus_tag="OO309_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1052 a          878 c          874 g         1002 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggaacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tctaattggt tatgatgcaa tggccgtcgg
      781 taatcatgaa tttgataatc ctctaaatat attacgtcag caggaaaaat gggccacatt
      841 tcctttcctt tctgctaaca tatatcaaaa aagtacaggg aggcgtttat tcagtccatg
      901 gaaaattttc atccgtcaga atttgaaaat tgcagtcatt ggcttgacga cagatgatac
      961 tgcgaaaaca ggaaattctg aatattttac tgatattgag ttccgtcaac ctgctgctga
     1021 ggcgaggagt gtcattgatg aacttaatca gcaggaaaag ccagatatta ttattgcggc
     1081 aacacatatg ggtcactatg acaatggcga gtcaggttcg aatgctcctg gtgatgttga
     1141 aatggctcgc agtttgccga ctggctcatt agcaatgatt gtgggaggac attcacaagc
     1201 tcctgtctgc atggccagcg ataataaaaa acagtggaat tatattccgg ggacaacctg
     1261 tgtaccagat aaacaaaatg gcatctggat tgtacaagcg catgagtggg gaaaatatgt
     1321 cggtcaggct gattttgaat tctgtaacgg tacaatgaaa ctggttaatt atcagttaca
     1381 cccagttaat ctcaaaatga gaattaccag agaagatgga aaaacggaat tttcttttta
     1441 cacccccgaa attactgaag atccacagat gttatcctta ctgacaccat tccagaataa
     1501 aggcaaagca caactggatg tgaaggttgg ggttgtgaac ggacgtctgg aaggggacag
     1561 aagtaaagtt cgttttgttc agaccagtat gggccatctg attctgagcg ctctgacaga
     1621 acgtattgac gcagattttg ctgttgtcag tggtggggaa attcgagatt ccatagagag
     1681 tggtaacatc acatacaaag atatactgaa agttcagcca tttggtaata cggtagtaag
     1741 tattgatttg acggggaaag aagtagctga ctacctagct acagtggcac agatgaaacc
     1801 ggattccgga gcctacccac aatttttgaa taccagcttt gtagtcaaaa aaggaaaaat
     1861 cgaaatgcta aaaataaaag gtaaatcggt tgaccttaac aaaaaatatc gtatgacaac
     1921 atttagcttt aatgctactg gtggggatgg ctatccacgt attgataata gacccggata
     1981 cataaatacc ggatttattg atgctgaagt acttatcgaa tatatccgga aacattcacc
     2041 gctggatgct gcttcatatg aaccaaaagg tgaagtcagt tggcagtgat aacgtaattg
     2101 acgatcatca cgtgtttttt gctgacagtc gctgtgactt tgtgaaagta gtctctgcgt
     2161 atgtttgcga tatgggaatg cagttgctgt attttgtgtt tctgcttctg ccagttgttg
     2221 ctgaatttaa ccttgcggct taactgatgt tggagcgctg ccagcttctt cgtcaatttc
     2281 gctgattaaa gtttattcat tatggcatga tctgcgtaag cggctcgtca gaaccatatt
     2341 gatatttact aagagctcag atcaactttc cagggcaaca gatcgcgtac ccggtttgcc
     2401 ggtcagtcct ggatatgctc aatgacgtaa cgcagccatt tttctggctc cacattgttc
     2461 agacggcatg tgccgatcag cgagtacaac accgccgcat gttcgccacc gctatcggaa
     2521 ccccgccgga gagagtttca ctctccggct gctcacggaa ccgtacgtga cactctcgcg
     2581 tcatacggct cttgatcact cattcgatgc acgtagtaaa taccagtggg caaatagctt
     2641 cggatactgt ttccttacca gcgccagctt tcgctgagcc ttccggttcg aaagtctgta
     2701 tttagactgc atccacttaa gcaaacgtga ctgcattgca ctccacagtc gatagctgaa
     2761 gtttctggac cagaactttc catagtactc aatccagcct ctcactatcg cattgtagcg
     2821 cctcgcaaaa tccagcaaac tctcagctgt tgagcgatgt atacgccact tcttgattgt
     2881 ttctgttatt ttgcacattg ctgcatttga tgcacccggc atacattttc tgtacaattc
     2941 gcctttgaaa ttcttcagag tacgcacttt gaagtcatat ccgagagtgg catacaacat
     3001 catcggcgta cctttcgaag ggaacgccgg gaaactgacg ttgcatccac atatcaaagg
     3061 catagtgcag gaacagattt gagagcaggg gggagattac gccgccctgc ggcgttcctc
     3121 gaccccgtgc cagcattgct ccatccggca tttgcaccgg tgccttcagc ctgcgctcaa
     3181 tgtacatcac cacccagcgt gcctgagtat gatgctgcac tgtcttcagt agcaggtcat
     3241 gatcgatatc atcaacgaag cctttaatat cgacttccac cacccagtca aacttccagc
     3301 agcgtttcct cgcctgcgca atcgcctggt gtgcggattt acccggtcga taaccatacg
     3361 agtctgcatg gaacaggggt tccagtaccg gctcgagtat ctgcttaacg accatctggg
     3421 cgatccggtc cgacacggcc ggaataccga gcggacgtac accaccgtca gctttgggaa
     3481 tgtccacacg ccttaccgcc tgcggcatat aactgccgga agccattctg ttccacagtt
     3541 tgtagagatt gtctgtaaca ttctcgtcaa acccggccag tgtctgcccg tcaatgcctg
     3601 ccgccccacg gttggctttt actcgtttat aagcttccca tacctgtcgt ttacttacag
     3661 gaaatggctt tgccgatttt acagcagtcc tcccatcggc attgtcgccc gtggttgccg
     3721 ccgcaccatc agcacaatgg ggcaagccct ttgctccgga cccattacgg tccttcaccg
     3781 ctactacggt ttactccgtc cctgta
//
LOCUS       DAJTQQ010000205         3726 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000205 DAJTQQ010000000
VERSION     DAJTQQ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.209"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..222
                     /locus_tag="OO309_004109"
     CDS             <1..222
                     /locus_tag="OO309_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5849022.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            268..477
                     /gene="hha"
                     /locus_tag="OO309_004110"
     CDS             268..477
                     /gene="hha"
                     /locus_tag="OO309_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5849023.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            516..1106
                     /locus_tag="OO309_004111"
     CDS             516..1106
                     /locus_tag="OO309_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR5849024.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1346..1606
                     /gene="repA"
                     /locus_tag="OO309_004112"
     CDS             1346..1606
                     /gene="repA"
                     /locus_tag="OO309_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR5849025.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1830..1904
                     /gene="tap"
                     /locus_tag="OO309_004113"
     CDS             1830..1904
                     /gene="tap"
                     /locus_tag="OO309_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR5849026.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1897..2754
                     /gene="repA"
                     /locus_tag="OO309_004114"
     CDS             1897..2754
                     /gene="repA"
                     /locus_tag="OO309_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR5849027.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3455..3646
                     /locus_tag="OO309_004115"
     CDS             3455..3646
                     /locus_tag="OO309_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849028.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRVIQAAFNAFAKV"
BASE COUNT         1018 a          907 c          935 g          866 t
ORIGIN      
        1 tttcagcgag atcgttatgg tcgtattctc gggcaggttt atgcaccaga cggaatgaat
       61 attaatcagt ttatggtccg ggccggggct gcctgggtat atgagcagta caacacagat
      121 cccgtgctac ctgtccttca gaacgaagcc agacaacaaa aacgtgggct ctggtcagat
      181 gctgatccgg ttcccccctg gatatggagg caccgtaaat aaagcgatgg caatcctatc
      241 attcatctgt tctcagcggg aataaaaatg gcaaaaacaa aacaggaatg gctgtatcag
      301 ttacggcgat gttcatcagt caatacgctg gaaaaaatta ttcataaaaa cagagacagt
      361 ttatcaaaca gtgaacgtga atcatttaat tcagcagcag accaccggct agcggaatta
      421 atcaccggaa agttatatga caggatacca aaagagatat ggaaatatgt aaggtaataa
      481 ttcagcaata attaattgcc aggagcagat aaataatgaa gttgattatt tttattctca
      541 ttgttctgat tattgcagcg ctacttatcc ggattatcct cagaagtgta aatcagcatt
      601 ctcctttgct gatgcaattg catgcggcag gtatacggac tggtgatgca gagcgaatac
      661 tgtccagcgg tgaatactgg caacgtcaga agacgctgct gacagaaagg gaagtcagtt
      721 ttatgaaagg actgttcaga attgtggata tgaagcggtg gtatctgtgt ccgcaggtac
      781 gggtagcgga tatcgtccag ctgaacggga atatccggcc acgatcacgc cagtggtggc
      841 agttattcag gatggtgtct cagtggcatg ttgatgtggt tatcgttgag ctgcgttcgt
      901 tcagtattgt tgcagcagta gagctggatg atgccagcca tttacgaccg gaacgcagac
      961 gccgggatat tcttctggaa gaggttctga ggcaggctgg tattccgttg ctcagaagcc
     1021 acgatgccag aaaactgctg cagatgaccg gagaatggct gaatacaaca ggggctgatc
     1081 agcagtcacc ggaacatcgt agctgacgcc ttcgcgttgc tcagttgtcc aaccccggaa
     1141 acgggaaaaa gcaagttttc ctcgctcccg gcgtttccat aactgaaaac catactattt
     1201 cacagtttaa atcacattaa acgacagtaa tccccgttga tttgtgcgcc aacacagatc
     1261 ttcgccacaa ttctcaagtc gctgatttca aaaaactgta gtatcctctg cgaaacgatc
     1321 cctgtttgag tattgaggag gcgagatgtc gcagacagaa aatgcagtga cttcctcatt
     1381 gagtcaaaag cggtttgtgc gcagaggtaa gcctatgact gactctgaga aacaaatggc
     1441 cgctgttgca agaaaacgtc ttacacacaa agagataaaa gtttttgtca aaaatcctct
     1501 gaaagatctc atggttgagt actgcgagag agaggggata acacaggctc agttcgttga
     1561 gaaaatcatc aaagatgaac tgcagagact ggatatacta aagtaaagac tttactttgt
     1621 ggcgtagcat gctagattac tgatcgttta aggaatttta tggctggcca cgccataagg
     1681 tggcagggaa ctggttctga tgtggatgta caggagccag aaaagcaaaa accccgataa
     1741 tcttctttaa ctttggcgag tcagaaagat taccggggcc cacttaaacc gtatagccaa
     1801 caatcaagct atgcggggag tatagttata tgcccggaaa agttcaagac ttctttctgt
     1861 gctcgctcct tctgcgcatt gtaagtgcag gatggtgtga ctgatcttca acaaacttat
     1921 taccgccagg taaagaaccc gaatccggtg ttcacacccc gtgaaggtgc ccggacgctt
     1981 ccgttctgcg ggaaactgat ggaaaaggcg gtgggcttca cctcccgttt tgatttcgcc
     2041 attcatgtgg cacatgcccg ttcgcttggt ctgcgtcggc gcatgccacc ggtactacgt
     2101 cgccgggcca ttgatgcgct gctgcagggg ctgtgttttc actatgaccc actggccaac
     2161 cgcgtccagt gttccatcac cacgctggcc attgagtgcg gactggcgac ggagtctgct
     2221 gccggaaaac tctccatcac ccgggccacc cgggccctga cgttccttgc agagctggga
     2281 ctgattacct accagacgga atatgacccg cttatcgggt gctacattcc gaccgatatc
     2341 acgttcacat ctgcactgtt tgctgccctc gatgtatcag aggaggcagt ggccgccgcg
     2401 cgccgcagtc gtgtggaatg ggaaaacaga cagcgcaaaa agcaggggct ggataccctg
     2461 ggtatggatg aactgatggc gaaagcctgg cgttttgttc gtgagcgttt ccgcagttac
     2521 cagacagagc ttaagtcccg tggaatgaag cgtgcccgtg cgcgtcgtga tgcggacagg
     2581 cagcgtcagg atattgtcac cctggtgaaa cggcaactga cgcgtgaaat ctcggaaggg
     2641 cgcttcactg ccagtcgtga ggcggtaaaa cgtgaagtgg agcgtcgtgt gaaggagcgc
     2701 atgattctgt cacgtaaccg taattacagc cggctggcca cagcttcccc ctgaaagtga
     2761 cctcctcaga ataatccggc acgcgccgga ggcattcgca cgcctgaagc ccgccagtgc
     2821 acaaaaaatc agcgctacat acaaaaaaca acctcatcat ccaccttcag gtgcatccgg
     2881 tcccccctgt ttttgataca aaacacgcct cacagacggg gaattttgct tatccacatt
     2941 aaactgcaag ggacttcacc ataaggttac aaccgttcac gtcataaagc gccagacgtc
     3001 aaccctacag ggtgcaatgt atcttttaaa cacctgttta tatctccttt aaactactta
     3061 attacattca tttaaaaaga aaacctgttc actgcctgtc ctgtggacag acaaatatgc
     3121 acctcccacc gcaagcggcg ggcccccaac cgaagccggt ttagttacaa catgcaaaca
     3181 taaaccccta aaaaacccaa gtccaacgca aaactgaaac cacaacgccc ccctcataac
     3241 tgaaaagcgc cgccgccccc gcccaaaggg cgggaacaga gtcgctttta attatgaatg
     3301 ttgtaactac atcatcatcg ctgtcagtct tctggctgga cgtatcgaat acacgctcgt
     3361 aagcggccct cacggcccgc taacgcggag atacgccccg acttcgggta aaaccctcgt
     3421 cgggaccact ccgaccgcgc acagaagctc tatcatggct gaaaacgggt atggcttggc
     3481 agggctgggg atgggtaagg tgaaatctgt caatcagtac cggctgacgc cgggcttcgg
     3541 cggttttact ccggtatcac atgtaaccac agcgtgccgc cttccatgcc gctggcgcgg
     3601 catcagagta atacaagcag catttaatgc atttgcaaaa gtgtgagtaa tattaagtgg
     3661 tcgtttcagg gatatcgtta agacttgagc gataggaatt aaaaccccaa aaagattaaa
     3721 aaaaca
//
LOCUS       DAJTQQ010000206         3488 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000206 DAJTQQ010000000
VERSION     DAJTQQ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.210"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..389
                     /locus_tag="OO309_004116"
     CDS             <1..389
                     /locus_tag="OO309_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR5849029.1"
                     /translation="HGVHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITEN
                     GSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGW
                     LTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(367..990)
                     /gene="tetR(B)"
                     /locus_tag="OO309_004117"
     CDS             complement(367..990)
                     /gene="tetR(B)"
                     /locus_tag="OO309_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5849030.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1072..2277
                     /gene="tet(B)"
                     /locus_tag="OO309_004118"
     CDS             1072..2277
                     /gene="tet(B)"
                     /locus_tag="OO309_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5849031.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2390..2983)
                     /gene="tetC"
                     /locus_tag="OO309_004119"
     CDS             complement(2390..2983)
                     /gene="tetC"
                     /locus_tag="OO309_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5849032.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3116..3487
                     /locus_tag="OO309_004120"
     CDS             3116..3487
                     /locus_tag="OO309_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5849033.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
BASE COUNT          948 a          641 c          716 g         1183 t
ORIGIN      
        1 accatggcgt acatgccaaa gtttccacgc cagtgcattt acgaaaagca cgtacttgct
       61 atgatcattt agctggcgaa gttgccgtta agatctatga ttccctttgt caacagcaat
      121 ggatcactga aaatggttca atgatcacat taagtggtat tcaatatttt catgaaatgg
      181 gaattgacgt tccttccaaa cattcacgta aaatctgttg tgcgtgttta gattggagtg
      241 aacgccgttt ccatttaggt gggtacgttg gagccgcatt attttcgctt tatgaatcta
      301 aagggtggtt aactcgacat cttggttacc gtgaagttac catcacggaa aaaggttatg
      361 ctgcttttaa gacccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc
      421 aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg
      481 taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg
      541 ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa
      601 tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc
      661 atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag
      721 taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc
      781 taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa
      841 agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc
      901 gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt
      961 aatcacttta cttttatcta atctagacat cattaattcc taatttttgt tgacactcta
     1021 tcattgatag agttatttta ccactcccta tcagtgatag agaaaagtga aatgaatagt
     1081 tcgacaaaga tcgcattggt aattacgtta ctcgatgcca tggggattgg ccttatcatg
     1141 ccagtcttgc caacgttatt acgtgaattt attgcttcgg aagatatcgc taaccacttt
     1201 ggcgtattgc ttgcacttta tgcgttaatg caggttatct ttgctccttg gcttggaaaa
     1261 atgtctgacc gatttggtcg gcgcccagtg ctgttgttgt cattaatagg cgcatcgctg
     1321 gattacttat tgctggcttt ttcaagtgcg ctttggatgc tgtatttagg ccgtttgctt
     1381 tcagggatca caggagctac tggggctgtc gcggcatcgg tcattgccga taccacctca
     1441 gcttctcaac gcgtgaagtg gttcggttgg ttaggggcaa gttttgggct tggtttaata
     1501 gcggggccta ttattggtgg ttttgcagga gagatttcac cgcatagtcc cttttttatc
     1561 gctgcgttgc taaatattgt cgctttcctt gtggttatgt tttggttccg tgaaaccaaa
     1621 aatacacgtg ataatacaga taccgaagta ggggttgaga cgcaatcgaa ttcggtatac
     1681 atcactttat ttaaaacgat gcccattttg ttgattattt atttttcagc gcaattgata
     1741 ggccaaattc ccgcaacggt gtgggtgcta tttaccgaaa atcgttttgg atggaatagc
     1801 atgatggttg gcttttcatt agcgggtctt ggtcttttac actcagtatt ccaagccttt
     1861 gtggcaggaa gaatagccac taaatggggc gaaaaaacgg cagtactgct cggatttatt
     1921 gcagatagta gtgcatttgc ctttttagcg tttatatctg aaggttggtt agttttccct
     1981 gttttaattt tattggctgg tggtgggatc gctttacctg cattacaggg agtgatgtct
     2041 atccaaacaa agagtcatca gcaaggtgct ttacagggat tattggtgag ccttaccaat
     2101 gcaaccggtg ttattggccc attactgttt gctgttattt ataatcattc actaccaatt
     2161 tgggatggct ggatttggat tattggttta gcgttttact gtattattat cctgctatcg
     2221 atgaccttca tgttaacccc tcaagctcag gggagtaaac aggagacaag tgcttagtta
     2281 tttcgtcacc aaatgatgtt attccgcgaa atataatgac cctcttgata acccaagagg
     2341 gcatttttta cgataaagaa gatttagctt caaataaaac ctatctattt tatttatctt
     2401 tcaagctcaa taaaaagccg cggtaaatag caataaattg gcctttttta tcggcaagct
     2461 cttttaggtt tttcgcatgt attgcgatat gcataaacca gccattgagt aagtttttaa
     2521 gcacatcatc atcataagct ttaagttggt tctcttggat caatttgctg acaatggcgt
     2581 ttaccttacc agtaatgtat tcaaggctaa ttttttcaag ttcattccaa ccaatgatag
     2641 gcatcacttc ttggataggg ataaggtttt tattattatc aataatataa tcaagataat
     2701 gttcaaatat actttctaag gcagaccaac catttgttaa atcagttttt gttgtgatgt
     2761 aggcatcaat cataattaat tgctgcttat aacaggcact gagtaattgt tttttatttt
     2821 taaagtgatg ataaaaggca cctttggtca ccaacgcttt tcccgagatc tcatctattg
     2881 aaacagcttg atagcctttt tcaacaaaca atattcgtgc tgagttaacc agtgattgat
     2941 aggtactctt aaaattttct tgttgatgat ttttattttc catgatagat ttaaaataac
     3001 ataccgtcag tatgtttatg gtatcatgat gatgtggtcg tgacaatctt aagaacattt
     3061 aggttatttt atgtatattg aacagcattc tcgctatcaa aataaagcta ataacatcca
     3121 attaagatat gatgataagc agtttcatac aacggttatc aaagatgttc tattatggat
     3181 tgaacataat ttagatcagt ctttactgct tgatgatgtg gcgaataaag cgggttatac
     3241 caagtggtat tttcagcggc tgttcaaaaa agtaacaggg gtcacactgg ctagctatat
     3301 tcgtgctcgt cgtttgacga aagcggctgt tgagttgagg ttgacgaaaa aaactatcct
     3361 tgagatcgca ttaaaatatc aatttgattc ccaacaatct tttacacgtc gatttaagta
     3421 catttttaag gttacaccaa gttattatcg gcgtaataaa ttatgggaat tggaggcaat
     3481 gcactgag
//
LOCUS       DAJTQQ010000207         3471 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000207 DAJTQQ010000000
VERSION     DAJTQQ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.211"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..1838
                     /gene="betT"
                     /locus_tag="OO309_004121"
     CDS             <1..1838
                     /gene="betT"
                     /locus_tag="OO309_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HCR5849034.1"
                     /translation="NASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSW
                     FAMLFSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIY
                     AIVALILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGY
                     GVLQVNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALL
                     LLALVLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWW
                     LSWSPFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDL
                     ANTVQQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVAN
                     TPVWQRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRD
                     LTKRESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEEL
                     NKQGTISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQH
                     RYYRAEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            1901..3178
                     /locus_tag="OO309_004122"
     CDS             1901..3178
                     /locus_tag="OO309_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HCR5849035.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKT"
BASE COUNT          824 a          793 c          897 g          957 t
ORIGIN      
        1 cgaatgccag ctggttctac atccttgctg tggccctgat tttactgagt gtcacgttcc
       61 ttggactctc acgctacggt gatatcaagc tgggcccgga ccatgcgcag cctgatttca
      121 gctaccactc ctggtttgcg atgctttttt cggcagggat ggggatcggc ctgatgttct
      181 ttggcgttgc cgaacctgta atgcattatc tttcgccacc cgttggcact ccagaaaccg
      241 ttgcggcagc taaggaagca atgcgtctga cctttttcca ctggggactg cacgcatggg
      301 caatttatgc cattgtggcg ctgattctgg cgttcttcag ttaccgtcac ggtctgcctt
      361 taactctgcg ctccgcactc tatcccatta ttggcgatcg catatacgga cctgtaggac
      421 atgcggttga tattttcgct gttataggca cggtctttgg cgttgcgaca tcactgggtt
      481 acggtgtttt gcaggtgaat gccggtttga accatctttt cggggtgccc atcaatgaaa
      541 cggtgcaggt aattctgatc gtggtcatca cggggttagc gacgatttca gtggtgtccg
      601 gtctggataa gggaatacgt atcctgtctg aactcaatct gggtctggct ttgttgctcc
      661 tggcgctggt cctgtgtctg ggaccaaccg tgcttctgct gaagtcattt gtggaaaata
      721 cgggcggtta tctttcggaa ctggtgagta aaacgttcaa cctttacgcg tatgagccca
      781 agtcgagcaa ctggctgggg ggctggacat tactgtactg gggatggtgg ctttcatggt
      841 cgccgtttgt ggggatgttc atcgcacggg tctcccgcgg gcgaaccatt cgcgagtttg
      901 tcaccggcgt gctgtttgtt cccgcgggtt ttacgctaat gtggatgacg gtgtttggta
      961 acagcgcgat ctatctcatt atgaaccagg gggccacaga cctcgccaat actgttcagc
     1021 aggatgtggc gctggccctg tttaatttcc tggagcattt cccgttctct tctgtgctgt
     1081 cattcattgc aatggcgatg gtcatcgtct tctttgtaac gtctgctgat tcgggggcaa
     1141 tggttgtgga tactctggca tcaggtggag tggcaaacac acccgtctgg cagcgaatat
     1201 tctgggcctc gctcatgggc attgttgcaa ttgcgcttct ccttgccgga gggctaagtg
     1261 cgctgcaaac ggtgacaata gcgagtgcat tgcccttctc agtgatctta ctaatatcta
     1321 tatacggact tttaaaagct ttgcgccggg atttgaccaa gcgtgaaagc ctgagcatgg
     1381 cgacaattgc tcctacggct gcacgtaacc caattccttg gcagagaagg ttacgcaata
     1441 tcgcgtatct gccgaagcga tctcttgtga aacgttttat ggacgacgtt atccagcctg
     1501 ccatgacgct ggttcaggag gaactgaaca agcaggggac gataagccac attagtgatg
     1561 cagtcgacga tcgtattcgt cttgaagtcg atttgggcaa cgagctgaat tttatatatg
     1621 aagtgaggct tcgcgggtat atctcaccga ccttcgcgct cgccgcaatg gataatgatg
     1681 agcagcagac tgaacaacat cgatattatc gcgctgaggt ttatctcaaa gaaggcggtc
     1741 aaaattatga tgtgatgggc tggaaccagg aacagctgat taatgacata ctggaccagt
     1801 acgaaaaaca cctgcacttc ctgcacctgg ttcgttaata gcaacatgcc gtcctggggg
     1861 cggcaattat tatcccggcc gcaatatgag ggaatgcaga atgatttcac gctggaaatg
     1921 gatgctgaag cagacaatta aaaaactatg gttcagggca acgttattcg caattgtcgc
     1981 gataataacg gcccttttat caattctttt taaatcaatg atacctgagt cggtttccgt
     2041 gaaggttggt gcggaagcag tcgataacat tctgaacata ctggcatcga gtatgctggc
     2101 agtgaccaca ttttcgctga gtatcatggt cacagcctac ggttcagcca ctactaatgt
     2161 gactcccaga gctacgcgtt tagttgttga agacgtcacc acacaaaatg tactggccac
     2221 cttcatcggt tcttttctct tcagtctggt agggattatt gccctcaata tgggagctta
     2281 tggagaaagg gggagagtca ttttattcat tgtcacactg gttgtcattg ccttaatcct
     2341 catcacattg cttcgctgga tacagcattt gacctctctg gggagggttg gtgagacaac
     2401 ggcacaagta gaacaggcgg ccatcgaaac atttattgcg agagcaagaa atccctgtct
     2461 cggtggatat ccatggcttg agaacaatga acagccgaaa ggaacggttg cagtttatcc
     2521 gaagaagatt ggctatgttg aatatattga tatggtgaaa cttagcaagc tgctgaccaa
     2581 tgatccccgt catgtatacc tcgtggcgca gccaggcagt ttcatacatc cgtccatgcc
     2641 agttttgtac ctgagtcagg gccaggagtc gtcaatcaac accgatttac ttgagacgat
     2701 tattgtctcg gatgcacgtt catttgctca ggatcctcga ttttgtctta gcgtcatggc
     2761 cgaaatagcc tgccgggccc tttcccctgc agtgaacgat cctggaaccg ccattgatgt
     2821 cattggcaga ggtgttcgta tactttccac ttacgcgcag aataaatctg atgaaataga
     2881 agtgaaatat ccttcagtac atgttgcacc acttcagaat aacgatctac tggaagactt
     2941 tttctcacct gtcgcgcgcg atggtgccag tatgagggag attcagataa gagtccttaa
     3001 aggactgtcg atgctgagta aaggttggcc tgagatattt gctgaagccg cacataccct
     3061 ggcatttgaa acattagagc atgcaactcg tgctgaccat atagattctg atagatatct
     3121 aataaaatca acttattata atttatttag tggcgaatat tctaataaaa aaacatagct
     3181 gcagagcaag caattcggtt gttggcaagt caagggcaaa gcattcgcgt gagaatgggc
     3241 gtatgctttg tgccaaaagc aaacgttgct aattctgcac tgttcagcga tggcgcagta
     3301 gagacaggca ctgccactgt ccagtcggca aaagcgaaca ctttgccact gcggggataa
     3361 actgattgaa gctctcgcgc aaaatcactt attcttttgc gttaataaaa tgtaggagat
     3421 gggtcattga tcctacccac gtaatatgga cacaggccta agcgaggttc t
//
LOCUS       DAJTQQ010000208         3421 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000208 DAJTQQ010000000
VERSION     DAJTQQ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.212"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(68..592)
                     /gene="sat2"
                     /locus_tag="OO309_004123"
     CDS             complement(68..592)
                     /gene="sat2"
                     /locus_tag="OO309_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5849036.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(687..1160)
                     /locus_tag="OO309_004124"
     CDS             complement(687..1160)
                     /locus_tag="OO309_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5849037.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1492..2028
                     /locus_tag="OO309_004125"
     CDS             1492..2028
                     /locus_tag="OO309_004125"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCR5849038.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            2143..2469
                     /locus_tag="OO309_004126"
     CDS             2143..2469
                     /locus_tag="OO309_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5849039.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(2657..2896)
                     /locus_tag="OO309_004127"
     CDS             complement(2657..2896)
                     /locus_tag="OO309_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR5849040.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT          946 a          757 c          722 g          996 t
ORIGIN      
        1 cttccctcat gatgtttaac tcctgaatta agccgcgccg cgaagcggtg tcggcttgaa
       61 tgaattgtta ggcgtcatcc tgtgctcccg agaaccagta ccagtacatc gctgtttcgt
      121 tcgagacttg aggtctagtt ttatacgtga acaggtcaat gccgccgaga gtaaagccac
      181 attttgcgta caaattgcag gcaggtacat tgttcgtttg tgtctctaat cgtatgccaa
      241 ggagctgtct gcttagtgcc cactttttcg caaattcgat gagactgtgc gcgactcctt
      301 tgcctcggtg cgtgtgcgac acaacaatgt gttcgataga ggctagatcg ttccatgttg
      361 agttgagttc aatcttcccg acaagctctt ggtcgatgaa tgcgccatag caagcagagt
      421 cttcatcaga gtcatcatcc gagatgtaat ccttccggta ggggctcaca cttctggtag
      481 atagttcaaa gccttggtcg gataggtgca catcgaacac ttcacgaaca atgaaatggt
      541 tctcagcatc caatgtttcc gccacctgct cagggatcac cgaaatcttc atatgacgcc
      601 taacgcctgg tacagcggat cgcaaacctg gcgcggcttt tggtacaaaa ggcgtgacag
      661 gtttgcgaat ccgttgctgc cacttgttaa cccttttgcc agatttggta actataattt
      721 atgttagagg cgaagtcttg ggtaaaaact ggcctaaaat tgctggggat ttcaggaaag
      781 taaacatcac cttccggctc gatgtctatt gtagatatat gtagtgtatc tacttgatcg
      841 atcaggcttt tgtatatctc cccaccacct gaaacaatga catgatccgt tattttcttt
      901 aggttggtta aagcatcttt aattgatgga aagatcaata cgttctcatt gtcagatgta
      961 aaacttgaac gtgttacgac cgcatacttt cggttgggta atgctcccat tgattcaaaa
     1021 gtcttgcgtc caaccaacag ccattggtta taggtaatag ctttaaacag gagctgttca
     1081 cctttggcac tccatggaat atcagggcca ttcccgataa ctccattctt cgatatagct
     1141 accattagtg atagtttcac aattcttcct cagaggttaa cattttatta gtaagcatgc
     1201 tcgttttgtc acccgctgat gcttaccgtt aattaatata atcacttgta ttaaatagac
     1261 tttctttaaa aatacaagac actctgttat tacaaatcgt gcatgccgtc tatcctgctt
     1321 gcacgatgca cattatctca cccaaagaac tcataacatt ttaatttatt tagcattaaa
     1381 ttaaaactct accggtaaac aagcatctct aggcgtaaaa aacaggcact tttttaaatc
     1441 ccagctagaa taggctgtat aggcagacag ttgcaagaca aggagcgttt tatgtctaac
     1501 agtccatttt taaattctat acgcacggat atgcgacaaa aaggttatgc gctgaaaact
     1561 gaaaaaactt acctgcactg gattaagcgt tttattctgt ttcacaaaaa acgtcatcct
     1621 cagaccatgg gcagtgaaga ggtcaggctg tttttatcca gcttagcaaa cagcagacat
     1681 gtagccataa acacgcagaa aatcgcttta aatgccctag cttttttgta caacaggttt
     1741 ttacaacagc cgttgggcga tattgattat atccctgcaa gcaagcctag acggctaccc
     1801 tctgttatct ctgcaaatga agtgcaacgc attttgcagg ttatggatac tcgcaaccaa
     1861 gttattttta cgctgctgta tggtgcaggt ttgcgcatta atgaatgctt gcgtttgcgg
     1921 gttaaagatt ttgattttga taatggctgc atcactgtgc atgacggtaa gggtgggaaa
     1981 agcagaaaca gcctactgcc cacgcgccta atcccagcaa taaaataact cattgagcaa
     2041 gcgcggctta ttcagcaaga cgacaactta caaggcgtag ggccatcgct gccttttgct
     2101 ttagatcaca aatacccttc tgcttatcga caagcggcgt ggatgtttgt ctttccctcc
     2161 agcacgctct gcaaccaccc gtataacggc aaattatgcc gccatcatct gcatgactcc
     2221 gttgcgcgaa aggcattgaa ggcagccgta caaaaagcag gcatcgttag caagcgtgtc
     2281 acttgtcata catttcgtca ctcgtttgct acgcatctat tacaagcggg gcgtgatatt
     2341 cgcactgtgc aagaactctt agggcataac gatgttaaga ccacgcaaat ctatacgcat
     2401 gtgttgggtc agcattttgc cggcaccacc agtcctgcgg atggactgat gctacttatc
     2461 aatcagtaaa agtagataaa tatctttatg tagccctgag tagcccctga tactttttat
     2521 ggtgaaaaag ataaacaatc atagagggga taaatatgag ccagttctac cattgttcag
     2581 ataagtttaa gcaagatact gtcaatcaca gtgaccattc atggtcatta cacttcctcc
     2641 agttgccaga caatgcctaa cataaaaacg caaaagccca aaagtcagag cttaggtgtt
     2701 ctaggcgttt tctaagcgct gaagaaatac gtcttaccgt agattgcgtg cgtttctgaa
     2761 gcacattttc cttatacatg gctttatccc aggcagcttc atcaagctct tggagtaaca
     2821 agccagtaat aatgcgacac tctcgggttt gtagcgagcc gctggtgaga tcggagttat
     2881 agcggaaagg ggtcatttgt aattatttgt gggtggagga tagttaactt attattgtag
     2941 ttggaagagg ctgctttcca aacattagca cctcgtaatg cttgttactg tatattaatt
     3001 tatgctctta gcgcatgtta atgggctatt acaatgcata atgtccacat caagataact
     3061 gtggcttaac acctgtacaa gcgaaaaaaa tcaatcacct gtccggaaat acgggatcac
     3121 tgtaaagtgt tggtgaggca gtctaggagg gggatttaat ttgggatact gatgaaagga
     3181 ttatgatcag ttggatatag gatcgtacta agctgagcgg caggttagct agattagtta
     3241 accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc gtattagctt
     3301 acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat ccttaaaaac
     3361 tccatttcca cccctcccag ttcccaacta ttttgtccgc ccacagctgc cgaccagtgt
     3421 a
//
LOCUS       DAJTQQ010000209         3349 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000209 DAJTQQ010000000
VERSION     DAJTQQ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.213"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            20..898
                     /locus_tag="OO309_004128"
     CDS             20..898
                     /locus_tag="OO309_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5849041.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            895..2286
                     /locus_tag="OO309_004129"
     CDS             895..2286
                     /locus_tag="OO309_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5849042.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2283..2540
                     /locus_tag="OO309_004130"
     CDS             2283..2540
                     /locus_tag="OO309_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849043.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2635..2835
                     /locus_tag="OO309_004131"
     CDS             2635..2835
                     /locus_tag="OO309_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCR5849044.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2828..3208
                     /locus_tag="OO309_004132"
     CDS             2828..3208
                     /locus_tag="OO309_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5849045.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
BASE COUNT          886 a          726 c          969 g          768 t
ORIGIN      
        1 acattctgag gtgacagcga tgatgacgtt taacctgcgt gaacaacaaa aaagactaca
       61 ggcgcgaatg gatgagttac gggcagagat tgcatttgct cagaagggcg aaaagccatg
      121 gccttatcgt tcctgcctga tgcgtgaagg tcgcggatat tgcgaaaaac atggcgaata
      181 tcacacgcat atactggtgt ggagcgatcg taatggcgag gacagagaag aaatttcatg
      241 ctgccctgac tgcttaatcg ctgaggccaa cgatttgacc atggagctgt cgtccatcaa
      301 ggcggaagag ctgactgata acgccggaat tgccctgcgt tttcgggact gcgagtttga
      361 taattatctg gaggttaatc ctggcgcagc cagaaatctt gcggcctgtc gccgctatgc
      421 ggagaactgg ccagatatgc tggagaacgg taccagtctt gttatgaccg gcagttgtgg
      481 taccggaaaa aatcatctgg ctgtggctat ggcaaaacac atcatccgta actatctggc
      541 cagtgtggag atcaccgacg tgatgcgcct tacccgtgct gtgaaaaatt gctggcggaa
      601 tgacagcgaa aaaacagcgg atgaagttat tgagcgttat gcgtcaatgg atttgctgat
      661 catcgacgaa gttggcgttc agtttggcag cgcggctgaa atggctattt tgcaggaaat
      721 tatcaacgcc aggtacgaaa gtatcttgcc caccattctg atcagtaacc tctcaccgga
      781 agagttgtgg gcgttcatca gtccccgaat tgccgacagg atcacagacg ggggacgcaa
      841 ctggttgtcg tttaactggc ccagttaccg ttctcgtatc agaggtgtgg ctgcatgaca
      901 actccagtat ggcgtaacga tgaccttgaa ggcgctgtca tcggcgcgtt ctttctgcgc
      961 ggagcagatc ctgaagtgat ggatattctg gccacactgc cagcggacgt tttttctgta
     1021 cgagcgtatc aggatatcta tacaggcatc tgcagacagg cccgtgtttc aggagtgatt
     1081 gaccccgtgc tgttgtgtaa tgagatgccg gaacttgccc cggtgattac tgataccggg
     1141 cgtaaaacct gggtgaagtc ttcactggag cactatgttg cagcgttgcg gcgcaatgcc
     1201 gcactgcgcg atgcagaaaa aacactgaat gaggcgctgc agaaattacg tgatgcgcat
     1261 acctgtgaag cagctgaaga tgccctgaag gatgcgcaga acatgatggt cacactgtcg
     1321 acaggaaagg gcgtcattca gccggtacat attgatgatg tgcttccgga agtggttgag
     1381 cgtgttgaat gccggaatca ggggctggag aaatccagga cgttgatgac cggtattgat
     1441 gaactggacg caaaaacagg cggtatggag cccggagacc tggtattcat tgccgcccgt
     1501 ccttcgatgg ggaaaaccga acttgcgctg gacatcatcg acaaggtgac tgagcagggg
     1561 catggtgtgc ttctgttcac catggagatg gcgaacatcc agattggtga acgtatggtg
     1621 tctgcggctg gagggatgcc agtatcacgc ctgaaatctg tggctcactt tgaagatgaa
     1681 gactggacgc gtttctcaca aggggtgggg cggatgaccg ggcgcaatat ctggatggtg
     1741 gaccaggcga acctggccat tgatgagata tgcgcaacaa cgaaacacca cctgattaaa
     1801 tatccggaaa cggcgctggt ggtggttgat tatctcgggc tgataaaaac ccgaaccacg
     1861 gggcgtcatg accttgccgt gggtgaaatc tcaaaggggc ttaaaggcct ggcaaaatcc
     1921 ggtggttttc cgttgattgc gctgagccag ctctcccgcg gtgtggagtc cagacccaat
     1981 aaacgtccca tgaactcaga cctgaaaaat tccggagaaa tagaggcgga tgcagacatc
     2041 attctgatgc tttacaggga tgaagtgtac aacccggata cgcaggccag gggcatcgca
     2101 gaaatcaata tcacgaaaca acgtaacggt tctctgggaa cgatttaccg gcgtttttat
     2161 aacggacatt ttctgcctgt ggaccaggaa agtgcacggg tgctttccac tcccatgaag
     2221 cccggcaatc cgcgcagata cagtaataaa cgaactgaca gcagtaagat ggagcgtttc
     2281 ttttgaacaa ccagacaatg acttttaccc ctgaacaatt gcgtaaacac gcgcaggaaa
     2341 tgctgcgtca tgctgaacaa ctcgaaaaaa cgggcataac aaaagatgct atccgtaaag
     2401 atatggtacc cgcgcttcgg gaactgatgc aggcgaagca tcgcgcacag aaagcagtag
     2461 atgagctggt ggactgtgtg gcagagctgg aaaccagagt cggaaagttt gaaaaactgg
     2521 tgcaggaggc gctgcgctga tgcgccatga gtttgttttg ccctggcttg tgcgcgatca
     2581 ttatgtccct ggtggaaagc ccgggataaa aatcaccgaa ccggagagta tttgatgagc
     2641 gtcaaaattc aaacgatacc ggaactgctt atccagacac gaggtaatat gaccgaagtt
     2701 tcacgaatgc tgaactgtaa tcgcgccacg gtaagaaaat atgctgaaga taaagagggc
     2761 aaagggcacg ccattgttga tggtgttctg attgttcatc gtggatggga caggggtaaa
     2821 gacagtgatg cgtgatattc agcaggttct tgagcgctgg ggcgcgtggg tggctaataa
     2881 tcatgaggat gtgacctggt cgtccattgc tgccggtttt aagggattaa tcccttcaaa
     2941 agtaaaatcc cgcccgcaat gctgtgacga tgacgcgatg gttatatgtg ggtgtatggc
     3001 ccgccttaac cggaacaaca acgatctaca tgacttgctg gttgactact acgtgtttgg
     3061 gatgacattc atgacgctgg cgcggaagca tggatgctca gatggttata taggaaagaa
     3121 gctacaaaaa gccgaggggg tggtggaagg aatgttgatg atgttgggtg tccggttaga
     3181 aatggacaaa tatattgaac atccctgatg gtaaggggcg actttctaga aaatatttta
     3241 cgtacgtaaa aaacgggata ttctgttaag agtggttgct tcgccacaca gcttaaaccc
     3301 gcctccgggc gggtttttta tgcctgaaaa acggtacagg acgttaaac
//
LOCUS       DAJTQQ010000210         3225 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000210 DAJTQQ010000000
VERSION     DAJTQQ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.214"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..122
                     /locus_tag="OO309_004133"
     CDS             <1..122
                     /locus_tag="OO309_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5849046.1"
                     /translation="SQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            279..1028
                     /locus_tag="OO309_004134"
     CDS             279..1028
                     /locus_tag="OO309_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR5849047.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1050..1616
                     /gene="yieF"
                     /locus_tag="OO309_004135"
                     /gene_synonym="chrR"
     CDS             1050..1616
                     /gene="yieF"
                     /locus_tag="OO309_004135"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR5849048.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1670..3007)
                     /gene="adeP"
                     /locus_tag="OO309_004136"
     CDS             complement(1670..3007)
                     /gene="adeP"
                     /locus_tag="OO309_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR5849049.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
BASE COUNT          901 a          834 c          797 g          693 t
ORIGIN      
        1 aaagccagca aaaaaagctg gaagttcctt ttaccctgct gtggcataaa cggaacagcc
       61 ataatccgaa gatcgtctgg ttacgggaaa ccattaaaaa cctttacgcg tcgatggcat
      121 aaccgaatcg tatgaaacgc gacccaattt cacaataaaa tgtaaaaaag ttgtaataag
      181 cttgtctgaa tcgaactttt agccgcttta gtctgtccat cattccagta aatgattact
      241 cttgtattca taatggacca ttaagcatgg agcgaaaaat ggcgactcac tttgcccgag
      301 ggattttaac ggaaggacat ctgatttctg ttcgtctccc ctcccagtgt catcaagaag
      361 cccgaaacat tccccctcat cgtcaaagcc gttttctggc gtccagaggt ttactcgcag
      421 aactgatgtt catgctgtat ggcattggcg aattgccgga aatcgtcacc ctgccgaaag
      481 gtaaaccggt tttcagtgat aaaaatttgc cttcgttttc tatttcctat gccgggaata
      541 tggttggcgt ggcgttaaca accgaaggtg aatgtggcct cgatatggaa ctacagcgtg
      601 cgacgcgcgg gtttcatagc ccacacgcgc ccgataacca caccttttcc agcaatgaat
      661 cgctatggat cagtaaacaa aacgatccta acgaagcgcg ggcgcagctc attacgctgc
      721 gccgaagcgt gctaaaacta accggtgatg ttttgaatga cgatccgcgc gatctgcagc
      781 tgctgcccat tgccggacgc ctgaaatgtg ctcatgtaaa tcatgtagaa gcgttatgcg
      841 acgcggagga cgtgctggtg tggtccgtgg cggtcacgcc cgcgattgaa aagctcagtg
      901 tctgggagtt agatggcaaa cacagctgga aaagcctgcc ggatattcac agccgcgcca
      961 acaatcctac cagccggatg atgcgttttg cccagctctc taccgtgaag tctttttcgc
     1021 caaattgata gacaaccaca ggagtcatta tgtctgaaaa attgcaggtg gttacgttac
     1081 tggggagcct gcgcaaaggc tcatttaatg gcatggttgc acgtaccctg ccgaaaattg
     1141 ctccggcgag catggaagtc aatgcgttac cttccattgc cgacattccc ctgtatgacg
     1201 ctgacgtaca gcaggaagaa ggttttccag caacggttga agctctggcg gaacagatcc
     1261 gtcaggctga cggtgtggtg atcgtcacgc cggaatataa ctactcggta ccgggtgggc
     1321 tgaaaaatgc catcgactgg ctttcccgcc tgccggatca accgctggcc ggtaaaccgg
     1381 tgttgattca gaccagctca atgggcgtga ttggcggcgc gcgctgtcag tatcacctgc
     1441 gccagattct ggttttcctc gatgcaatgg tgatgaacaa gccggaattt atgggcggcg
     1501 tgattcagaa caaagttgat ccgcaaaccg gagaagtgat tgatcagggt acgctggacc
     1561 acctgaccgg gcaattgacc gcatttggtg agtttattca gcgagttaag atctaaataa
     1621 aaaacccgcc agtaatcatg catggcgggt ttttaatgcg ctatcgattt taatgagcgt
     1681 cgataaatac aatcttcagg ataaacagca gcgcaacgat gattacgcac gggctaaggt
     1741 cacgcagacg cccggtacca atcttcatca cgcagtagga gataaagccc agcgcaatac
     1801 cttcggtaat cgaaaagctg aacggcatca tcacggcggt aataaacgcc ggaacagatt
     1861 cagtaagatc ctgccagttc acgcgagcaa gacttgaggt catcaacacg ccaacgtaga
     1921 tcaacgcgcc agctgcagcg tagtctggca ccattcccgc cagcggcgac agaaagataa
     1981 ccagcaggaa cagcagacca acaaccactg ccgtcagacc ggtacgaccg ccaaccgata
     2041 cgccggaaga ggactcaata taagccgtaa cggaagaagt accgataaac gaaccggtca
     2101 cggaagagat actgtcgaca tacagcgcct gcttcatgcg cgggaatttc cccttctcat
     2161 ccgccagacc tgctttatcg gtcacgccaa tcagcgtacc ggaggagtca aacaagttga
     2221 ccaacatgaa agagaaaatc acccctgcca gcccgaggtt aaacgacccg gctaaatcta
     2281 catgacccac aactgtcatt acgctcggcg gcgcagaaac gatgccattg tagtgcacat
     2341 cacccagcat ccagcccagc agcgtcgtca ccacgataga aaccagcacc gctgcgtgaa
     2401 tgttgcgcga ggccagaata gcaatgatga aaaagccgag gatacccaga agtacgctgt
     2461 gagaagtcag attaccgatg ctcaccagcg tttccgagtt agcgacaatc acacctgcgt
     2521 ttttcagccc catcatgccg ataaacaggc cgataccgct ggtaataccc acacgcagac
     2581 ttaccggaat gttagcaatc atccagtaac gaacgcggaa aatggtcaaa agaagcagtc
     2641 cgacagcgcc ccagaagatt gcgcccatac cgatctgcca cggcagcccc attgcctgga
     2701 caaccacaaa agcgaaaaag gcgttaagcc ccatcgcagg agccagcgcc accggaaggt
     2761 ttgcaaacaa tcccatcata atgctaccga aagcagcaat cagacaggtt gttacgaaga
     2821 cggcactggt gtccatgcca gcaacgccca gaatttgtgg gttaacaaat acgatgtaga
     2881 ccatcgtcag gaaggtggtg aacccggcaa tcacttcagt tcgtgccgta gtaccatgtt
     2941 cgcgcagttt aaaaacacgt tccagcatcc tctgaccaga agtctgggtg gtatgttgat
     3001 gactcattat ctatttccga acaaggaggg aaaatccgtc gctatcgtat accaaaatgc
     3061 gacaataggc gcgtttgtga gagacttttt tattggattt acttatacgg caacgattgc
     3121 gttgcgcaaa tcggctctac gaaaacgtta aactgattaa aaaggaaagg catgtcccgg
     3181 atagaagcgg tatttttcga ctgcgacggt acgctggtcg acagt
//
LOCUS       DAJTQQ010000211         3221 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000211 DAJTQQ010000000
VERSION     DAJTQQ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.215"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..147
                     /locus_tag="OO309_004137"
     CDS             <1..147
                     /locus_tag="OO309_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5849050.1"
                     /translation="AIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKY
                     KGAV"
     gene            484..2097
                     /gene="mppA"
                     /locus_tag="OO309_004138"
     CDS             484..2097
                     /gene="mppA"
                     /locus_tag="OO309_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR5849051.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVSQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2148..3179)
                     /gene="ynaI"
                     /locus_tag="OO309_004139"
     CDS             complement(2148..3179)
                     /gene="ynaI"
                     /locus_tag="OO309_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR5849052.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT          923 a          707 c          734 g          857 t
ORIGIN      
        1 gctattaaag aaaaaaaact gatacgtgtt ttggataaat tcactccaga tttacccggt
       61 tatcacctgt actatccaca ccgtcgacat gctggctcgg cattctcatt atttatagat
      121 aggctgaagt ataaaggtgc tgtttagcac tacttgctga tacattaatt taattcttct
      181 cttaacgtat tctcagttcc tttcaacgtt ttggttattt tttattcttc gtacaatggc
      241 gacagatgct gattatgata ccgaaaactg gtttgaacgt gcgaagcccg aacgtagtgt
      301 tcggtgttct atgtgctttg ccacattttg gcgtctatta tttacactaa atctgatttg
      361 atatattgat acttaaaaca tttgatgctt cctttgtcac ttttttgatg gaagttgttt
      421 gcatttcttt aaggcgaaac aaataattac gcatcaattt taatgtcggt tagagggaaa
      481 cttatgaagc actctgtttc agttacgtgt tgtgcgctgt tggtcagcag catttctctt
      541 tcgtatgctg cagaagttcc gagcggcaca gtactggcag agaagcagga gctggtgcgc
      601 cacattaaag atgagcctgc gtcgctggat cccgctaaag ccgtgggcct gccagagatt
      661 caggtcattc gcgatctgtt tgaaggtctg gtgagtcaga acgaaaaagg ggagattgtc
      721 cccggcgtcg cgactcaatg gaaaagtaat gacaaccgta tctggacttt taccctgcgc
      781 gataacgcaa aatgggcgga tggcacaccg gtaacggcgc aagattttgt ctacagctgg
      841 caacgtctgg tggacccaaa aacattgtcg ccatttgcat ggtttgccgc gctggcggga
      901 atcaacaacg cacaggcgat tattgatggt aaagctacgc ctgatcagct tggcgtcacc
      961 gcagttgatg cccatacttt gaaaattcag cttgataaac cgttgccgtg gtttgtgaat
     1021 ttaaccgcta actttgcctt cttcccggtg caaaaagcca acgtagaaag cggtaaagag
     1081 tggacgaaac ccggaaatct gatcggcaat ggcgcttatg ttcttaaaga gcgcgtagtc
     1141 aatgaaaaac tggtcgtggt accgaatacc cattattggg ataacgccaa aacggtgctg
     1201 caaaaagtga ccttcctgcc aattaatcag gaatccgcag ccactaagcg ttacctcgcg
     1261 ggggatattg atatcaccga atccttcccg aaaaatatgt atcagaagct gttgaaggat
     1321 attccggggc aggtttatac gccaccgcag ctcgggacct attattatgc ctttaacacg
     1381 caaaaagggc cgacggcaga tcagcgcgtt cgcctggcat taagtatgac gatagatcgc
     1441 cgcctgatga ccgaaaaagt gttggggacg ggcgaaaagc cagcgtggca ttttatacca
     1501 gatgttaccg ccggatttac gccggaacct tcgccgtttg aacaaatgag tcaggaagaa
     1561 ctgaatgcgc aggcaaaaac tttgttgagc gcagctggtt atggtccgca aaaaccgctg
     1621 aagctgacgc ttttgtataa cacttcagaa aaccatcaaa aaattgcgat tgctgtagca
     1681 tcgatgtgga aaaagaacct tggcgtagat gttaaattgc aaaatcagga atggaaaacc
     1741 tatatcgata gccgtaacac cggcaatttt gatgttatcc gcgcctcatg ggtgggggat
     1801 tataatgaac cctccacttt cctgacatta ttaacgtcaa tgcattcagg aaatatttca
     1861 cgctttaaca atccggcata cgacaaagtt ctggcccagg catcgacgga aaataccgtt
     1921 aaagcgcgta atgccgatta caacgcggca gaaaaaatcc tcatggagca agcaccgatt
     1981 gcaccaattt atcaatatac caatggacga ttaatcaaac cgtggctgaa aggttatccc
     2041 attaataacc ctgaagatgt agcgtacagt cggactatgt atattgtgaa gcattgatgt
     2101 gatgggaact ggcgttaccc ttgtggataa cgccagtgat gtctgattta gcgcccctgt
     2161 tccggcggtg taatgttatc catatacagc gtctggctgg ggaaggcaaa gtccgcgccg
     2221 tgtgactgta caatatcgat aatcttcaaa taaacgtctt gctgtgcagc aagccactca
     2281 gcccataccg tggttttggt aaagcaataa accataatat tcaatgacga gtcagcaaac
     2341 tggttgaaat aaaccagtaa ggtttgtcgc tggtcgatgg ccgggtgatt tttcagcatc
     2401 tcacgtacgg cttcgacaat aacgcccact tttgccgcat cctcataacg taaaccaatg
     2461 gtcgtggtaa tgcggcggtt ggtcattcgt cctgggtttt ctacgctgat cgacgaaaac
     2521 agcgagttcg gtacgtacaa tggacgatta tcaaaggtcg taattttggt aattcgccag
     2581 ccaatttccg ctactgtacc ttcgatattt ctgtccggtg aacggatcca gtcgccaata
     2641 ctgaaaggac ggtcgaaata gagcatgatc ccggaaaaga aattactcag aatatcttta
     2701 ccggccatac cgacagccag accaccaata ccaccaaagg tcagcaagcc agaaaggctc
     2761 atgccgaaat gttcgccata aagcagaaca agcactacaa taatggtgat tttgataatg
     2821 cgcgacataa tccgcgcact ggtgatatcg cgaccttttt taatctgctg tttttcaaac
     2881 tgattaatca gcagaaatag cttaatcgtc agaataaccg caatcaggga cgtacagata
     2941 aaatcgataa cgcctggggt gataaatttg agtttatagt tttctataac ataattactg
     3001 atgctaccaa cagcactgat aattatggtg tagattaaaa attgcaccgc atggaataaa
     3061 aatccttttc ttttacgatt tccacggcga aaccaaaagc tcatcagaat caatgctgcg
     3121 cagctaccga aaataatgac cagattaagc gcattatttg taaacagttc agcgatcatt
     3181 gttttatcag gctcctccag ataattgtcg tcatgccgga a
//
LOCUS       DAJTQQ010000212         3201 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000212 DAJTQQ010000000
VERSION     DAJTQQ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.216"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            102..3005
                     /locus_tag="OO309_004140"
     rRNA            102..3005
                     /locus_tag="OO309_004140"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            3101..>3201
                     /gene="rrf"
                     /locus_tag="OO309_004141"
     rRNA            3101..>3201
                     /gene="rrf"
                     /locus_tag="OO309_004141"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          851 a          702 c          992 g          656 t
ORIGIN      
        1 tcaagctgaa aattgaaaca ctgaacaacg aaagttgttc gtgagtctct caaattttcg
       61 caacacgatg atgagtcgaa agaaacatct tcgggttgtg aggttaagcg actaagcgta
      121 cacggtggat gccctggcag tcagaggcga tgaaggacgt gctaatctgc gataagcgtc
      181 ggtaaggtga tatgaaccgt tataaccggc gatttccgaa tggggaaacc cagtgtgatt
      241 cgtcacacta tcattaactg aatccatagg ttaatgaggc gaaccggggg aactgaaaca
      301 tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc cagtagcggc gagcgaacgg
      361 ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga agcgtctgga aaggcgtgcg
      421 atacagggtg acagccccgt acacaaaaat gcacatactg tgagctcgat gagtagggcg
      481 ggacacgtgg tatcctgtct gaatatgggg ggaccatcct ccaaggctaa atactcctga
      541 ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa agaaccccgg cgaggggagt
      601 gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag cacgcttagg cgtgtgactg
      661 cgtacctttt gtataatggg tcagcgactt atattctgta gcaaggttaa ccgaataggg
      721 gagccgaagg gaaaccgagt cttaactggg cgttaagttg cagggtatag acccgaaacc
      781 cggtgatcta gccatgggca ggttgaaggt tgggtaacac taactggagg accgaaccga
      841 ctaatgttga aaaattagcg gatgacttgt ggctgggggt gaaaggccaa tcaaaccggg
      901 agatagctgg ttctccccga aagctattta ggtagcgcct cgtgaattca tctccggggg
      961 tagagcactg tttcggcaag ggggtcatcc cgacttacca acccgatgca aactgcgaat
     1021 accggagaat gttatcacgg gagacacacg gcgggtgcta acgtccgtcg tgaagaggga
     1081 aacaacccag accgccagct aaggtcccaa agtcatggtt aagtgggaaa cgatgtggga
     1141 aggcccagac agccaggatg ttggcttaga agcagccatc atttaaagaa agcgtaatag
     1201 ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg caccgaagct
     1261 gcggcagcga cgcttatgcg ttgttgggta ggggagcgtt ctgtaagcct gtgaaggtgt
     1321 gctgtgaggt atgctggagg tatcagaagt gcgaatgctg acataagtaa cgataaagcg
     1381 ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc caacgttaat cggggcaggg
     1441 tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat gggaaacagg ttaatattcc
     1501 tgtacttggt gttactgcga aggggggacg gagaaggcta tgttggccgg gcgacggttg
     1561 tcccggttta agcgtgtagg ctggttttcc aggcaaatcc ggaaaatcaa ggccgaggcg
     1621 tgatgacgag gcactacggt gctgaagcaa caaatgccct gcttccagga aaagcctcta
     1681 agcatcaggt aacatcaaat cgtaccccaa accgacacag gtggtcaggt agagaatacc
     1741 aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa tggtgccgta acttcgggag
     1801 aaggcacgct gatacgtagg tgaagcgact tgctcgtgga gctgaaatca gtcgaagata
     1861 ccagctggct gcaactgttt attaaaaaca cagcactgtg caaacacgaa agtggacgta
     1921 tacggtgtga cgcctgcccg gtgccggaag gttaattgat ggggttagcg caagcgaagc
     1981 tcttgatcga agccccggta aacggcggcc gtaactataa cggtcctaag gtagcgaaat
     2041 tccttgtcgg gtaagttccg acctgcacga atggcgtaat gatggccagg ctgtctccac
     2101 ccgagactca gtgaaattga actcgctgtg aagatgcagt gtacccgcgg caagacggaa
     2161 agaccccgtg aacctttact atagcttgac actgaacatt gagccttgat gtgtaggata
     2221 ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag ccgaccttga aataccaccc
     2281 tttaatgttt gatgttctaa cgtggacccg tgatccgggt tgcggacagt gtctggtggg
     2341 tagtttgact ggggcggtct cctcctaaag agtaacggag gagcacgaag gttggctaat
     2401 cctggtcgga catcaggagg ttagtgcaat ggcataagcc agcttgactg cgagcgtgac
     2461 ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg gttctgaatg gaagggccat
     2521 cgctcaacgg ataaaaggta ctccggggat aacaggctga taccgcccaa gagttcatat
     2581 cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat cctggggctg aagtaggtcc
     2641 caagggtatg gctgttcgcc atttaaagtg gtacgcgagc tgggtttaga acgtcgtgag
     2701 acagttcggt ccctatctgc cgtgggcgct ggagaactga ggggggctgc tcctagtacg
     2761 agaggaccgg agtggacgca tcactggtgt tcgggttgtc atgccaatgg cactgcccgg
     2821 tagctaaatg cggaagagat aagtgctgaa agcatctaag cacgaaactt gccccgagat
     2881 gagttctccc tgacccttta agggtcctga aggaacgttg aagacgacga cgttgatagg
     2941 ccgggtgtgt aagcgcagcg atgcgttgag ctaaccggta ctaatgaacc gtgaggctta
     3001 accttacaac gccgaagatg ttttggcgga ttgagagaag attttcagcc tgatacagat
     3061 taaatcagaa cgcagaagcg gtctgataaa acagaattag cctggcggcc gtagcgcggt
     3121 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt
     3181 ggggtctccc catgcgagag t
//
LOCUS       DAJTQQ010000213         3183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000213 DAJTQQ010000000
VERSION     DAJTQQ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.217"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            1..442
                     /locus_tag="OO309_004142"
                     /pseudo
     CDS             1..442
                     /locus_tag="OO309_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1026..1742
                     /locus_tag="OO309_004143"
     CDS             1026..1742
                     /locus_tag="OO309_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849053.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2294..2698
                     /locus_tag="OO309_004144"
     CDS             2294..2698
                     /locus_tag="OO309_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849054.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2761..3183
                     /locus_tag="OO309_004145"
                     /pseudo
     CDS             2761..3183
                     /locus_tag="OO309_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          892 a          653 c          724 g          914 t
ORIGIN      
        1 gcgttctaag ccttcccacc atgatcgggg aagccccaga tatgaacagg cacattcaga
       61 gttactttaa agagcttact gaacgtctga ggtgttagag ccatgactgc cgcagcattg
      121 ctggcggaaa tgcctgaatc gagcagcctc tcgagacgtg aaatcagcgc acttgtcggt
      181 gttgctcagg taaaccggga ctcgggaact ctgcgcggtc gacgaaccat tttcggtgat
      241 tgtgctgggg aagaacagct ctgtacatgg cggcgcttga ggcccccccc ggtttaatct
      301 ggtgataaag gcgttttaca tgcgcctact tgcagccgga aacgccaaaa aagtggcact
      361 ggttgcctgt atgcgtaagt tgctgaccat tatgaacgca atgctcagaa aaaacgaaga
      421 atggaatgaa tcgtatttat gaaaactgga ttgtgggcca taaatattcc aaaaagatag
      481 cccgctccgt ttattaccgg aacgggcgag agcatgacat tgtctgttat tataatgaga
      541 ctgctgattc tacttatttt taatttcaag aaaagcgttt tctacaaaga attatgagat
      601 ttaatgtttt gtttagacaa tattatcatt ctgttcaata atgaaagtct tcatcaacaa
      661 caagtaaaaa caacgtccct ctcatcgaac cggacttgca gatttcccgc atccggctct
      721 cttgcaacac atcagacctt cacgttcggg cggttatggg gaaggtccag caagctgtat
      781 gagccctaac tgtttgaata ccagtagatt accagaaact tctatcgatt gaagacatta
      841 ccaactagcg ttactaaatg ttgaccaagt tttgttcact tgtctggttt tgatagtagc
      901 attctgcaga gtattttgat atgataaata tgcattaaca atgatattat tgttaatttc
      961 ctagcatgaa aattaaaaga tcaactttta tctcgaatat tttttacatt atcagttagt
     1021 ttcttatgaa tgacaattca ttgcttagaa attcttcatt attcatcgcc tatatgggat
     1081 gtgtagggtg ggtcagtgct tattcttatg gctggggaac ctctttttac tatggatttc
     1141 catggtgggt tgttggtgca ggtttggatg atgttgccag aagtttactc tatgctatca
     1201 tagtaatggg tatacttttt accggttggg ggattggtat tttatttttt cttcttataa
     1261 agaaaagaag taaaatacaa gacttgagtt tttttagact attttttgct attaccctac
     1321 tgtttttccc tgtaatattc gaacttttaa ttctcaaaca atatttcata cttccattat
     1381 cactttcatt cattattagt tcattggtta tttcaataat aattcgtata tatggacgga
     1441 tcttttctgt atcatgtttt tcagatatcc cttttgttag agaacaccgc attaagttaa
     1501 ttatggctgg ttttttggtt tatttttggt ttttttcctt tcttgttggc tggtataaac
     1561 ctcaattaaa aaaagaatat cagatgcttt gttataataa tagttggtat tatgttcttg
     1621 ctcgttatga ttcacggctc gttttatcta gttcatttaa agatgacagt aacagattct
     1681 taatatttaa tacagaacag tcgggatttt atgaaatcaa tgatgtttat gtcagaaagt
     1741 aataatcctc tcccacaaag tgaattaaat gaatatataa tgcgttcgaa gtatatgtat
     1801 tgagctgtca tactgagaaa caaaccttag ggcagcacaa ttaaagccag aaacaactat
     1861 gcaagaaagt tactaaataa gtgctcggaa cagtaaaaca ctcagggggg actcagtccg
     1921 gattatgtaa tcagataaat tgccaaacat cacaaatcac cagccggact gagtgatgcc
     1981 gagcattgca ccaatacccc gtgatgaacg atgcctgatg cagaaagctc cctataaaac
     2041 aaagaacaaa aactacgacc ggcgattcac cgtcatactg atgctgcatc gtggtgacac
     2101 tgtctgtcac tttcatgttg actgaacaac tctgctgcga agaaggtgta ccgccggcgg
     2161 gatttgattg tccggcgtct agcttccact tccctgatgg aactgaaagt tgttatggcc
     2221 ggtcagcttg ccctgtccgc aggacatgag gtgctgtgac ggacagcacc agtaacggaa
     2281 agtgacaatg aaaatgaata ctgcgctgat agttgcacta atgtgtatgt ggtatgcggt
     2341 acccgctgct gcaaaagaga ctttactggc tatgccacgt aattcaacag aacattgcta
     2401 tgcggagatt aatgtgcacg gaccatatgg ggtgtatttc cgagtggtcc cgcatccccc
     2461 cgggggaaag agctgggtcg aatgtaacag tgactactat tactctgata aaccaccggg
     2521 agtacagata ctcggaaccc gtgcagggtg tcgggtatac ggaatctgcg ggaccacctc
     2581 gacactgcat gtggctggaa gaggggttgt gtgtattaaa aatatatgca gtccacgtgg
     2641 gatgataatt cacaggatac ggaaacgtcc tgtagtggct gtaagcgatg agatgtgatc
     2701 ttacccagca atagtagaca cgcggctaag tgagtaaact ctcagtcaga ggtgactcac
     2761 atgacaaaaa cagtatcaac cagcaaaaaa acacgtaaac agcattcgcc tgaattttgc
     2821 agtgaagccc tgaagcttgc tgaacgcatc ggtgttgctg ccgcagcccg tgaactcagc
     2881 ctgtatgaat ctcagctcta tgcgtggcgc agcaaactgc agcaacagat gacctcgtct
     2941 gagcgtgaaa gtgaactgcc cgcctgaagc ggcaactggc ggagcaggca gaggaactgg
     3001 ctatcctcca aaaggccgcg acatactttg cgcagcggct ggtatacgtg gtgtcagcgt
     3061 cggacaggaa taagccctcg tcagcagttc cgtcagcact gcgacagcgt tgtcctcgcg
     3121 gcggccttta cccggtcaaa acagcgttac ggtgcaccac gcctgacgga tgaactgcgt
     3181 gct
//
LOCUS       DAJTQQ010000214         3179 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000214 DAJTQQ010000000
VERSION     DAJTQQ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.218"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            418..1527
                     /gene="fepE"
                     /locus_tag="OO309_004146"
     CDS             418..1527
                     /gene="fepE"
                     /locus_tag="OO309_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR5849055.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2552..2746)
                     /locus_tag="OO309_004147"
                     /pseudo
     CDS             complement(2552..2746)
                     /locus_tag="OO309_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          801 a          659 c          774 g          945 t
ORIGIN      
        1 aaaaaaaaca ttatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg gattttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg ggggggaccc tgagcaggtg gtaattgtaa gacgttgcgg
     2821 gcgacggtat tacaattgca catcctgtcc tgtttcttta ggacgtatca tggcacagca
     2881 tatcaactga taaataatcc ctgtgcagtg cagccgctgt tgcataaaat ttcgctctgc
     2941 gtcgagtgtt tcttaatcaa taattccgct gatactccgg aaaaacgcga gtctgttctc
     3001 atgcctgaaa tgcccacacc ccacgcaaaa acaagttttt gctgattttt ctttataaat
     3061 agagagttat gacaaattag ttcttcttgc tctctttgtg atatttaaaa aagcggtgtc
     3121 ggcgcggtgt tgtagctgcg ccaacaccgc tttttagggg tggtactgac tattttcat
//
LOCUS       DAJTQQ010000215         3178 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000215 DAJTQQ010000000
VERSION     DAJTQQ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.219"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(185..1894)
                     /locus_tag="OO309_004148"
     CDS             complement(185..1894)
                     /locus_tag="OO309_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR5849056.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIIAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2326..3045)
                     /locus_tag="OO309_004149"
     CDS             complement(2326..3045)
                     /locus_tag="OO309_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR5849057.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT          969 a          606 c          534 g         1069 t
ORIGIN      
        1 tttttctgct cattattagc tccttctgat gccattctat ttcaggaagg agtgtccgtt
       61 aaactcaggc tacctcaggt tgccaattga ggtggttgcc tgaaagttgg cagttacccg
      121 gtcactacac cttgttggag tgaacatatc aaaaacaggt aattacacaa tttaaattac
      181 agtctcataa aaaatgatta aatctattta ttatttcaat aaacgttttt ttcatcttct
      241 ttgaaaattg ttctgctata gtatccggtt ccagattgtt ctcttttatt attctacaat
      301 actctaatat tgtttctttg tctctattta ctacagatac aaatacacca ggaataccat
      361 ctttattagt tgcggacagc atttcgacca atcgtcttgt ggtaaaacta tagtttttta
      421 gaatattaca aaaggcacgt attgtttcgg tatggccatt ataaaatgcc ataaaaagtc
      481 ctgatgtacc gttgggattt tttatccata ataattcatc tatatattcc tggtgagggg
      541 ggagtatttt tagtatatct ccatacgcta taatggcctt tgtgtgccca ttttgaagtg
      601 ccataaacaa cccatgcttt actttcaata attctttaat ttgttcacta ctaagcattt
      661 tcttattaag cagacttccg aacatacgaa tactatctgc atgtccattc tgcaatgcaa
      721 aaaataaccc aggtaaatca tggctgtctt tcgcagctaa tagatataat ctcttatcgg
      781 aagttaacgg ggttttaaag actatttccc cataggcttt tattgtgtct acatgtccgt
      841 tcataaaaga catgcataaa cccggcacat tgtttgcccc taccgcagca agcaatgtaa
      901 gtattgtttc ttgactaaga ttgcacatat ttaatatctt accatatact tgaataatgt
      961 ctgaatatcc gttttgaagt gctatataaa atgcaggagt accatcagag cttctcgtgg
     1021 ccagaaggtt caccagttta gattgagaaa tttcaccatt tttgtatttt tccatcagta
     1081 taaataatac ttctgtcaga ttctttgtta tcgcatgata tataactctg gaattgatca
     1141 attcatctgg taaaagtttc actgtgtttg ttagagggga attatgccta tcttcaaata
     1201 ttacctgccc gggttgctcc agaccataaa ttttccaata aaaatcatca aaaaaatcct
     1261 tagcggataa ttgactcagt tcgaaatggc tatcacaagt aaatacagta cgtttatgag
     1321 taacagttcg attaggatcg aaaaactgaa caacccattt ttcactttct tgtacgcgct
     1381 ttattttcaa ccctaacgcc atttggtggt ttaatgtgca tgctgataat gttctgacaa
     1441 agtctccatt ttttttcatg ttataaaaaa aattagtaat aactgttccc cactttttat
     1501 tttccacaaa ataaatataa gctggtgatt ttgaaaaatc ttccgttttt tccaaacata
     1561 cattttttgc aatagcatca ggggtgctaa gaagctgata gtttaccttc ccatttgttt
     1621 ccataaaatt catttcccaa taggaagcta ggtgacggca aactatttgt cttccatata
     1681 tatcttttgc cactccattt atatttacct ccacatttct cttttctgaa aaataaggtg
     1741 ctgttttatt cttaaactga attagttcac tggagtttga gaccctcata atagaacttg
     1801 ttattgttct attatctctg ttaacagaca tatctgttga taacgaattt tttgttgaaa
     1861 atatactaga tgaaaatgtt ttatttaacg gcatagaaga caaccataga gatgttataa
     1921 tactattaga ttgcagttac taatagtaaa agcctcctta caaggaggta gcttattcag
     1981 aatgctccta taagatagcg agctcgtcaa cgatacagat gatctgatta tattgtgcaa
     2041 aaggagaaca ggaaaatagg cactggaaaa gatgaaggcc tgatgggagc attaactaat
     2101 gcaaaatccg acaggttaat atcccgaaaa gcaatcaaac gactcaaaca ggagatgcac
     2161 gctgaaatgg tggacaactg ttcggatttc agatgagtag ctcagcagtt tatgagccga
     2221 atgctctgtc acgggcagga atcaggctgc atacgcagtg ccaaatctaa tcgtagttat
     2281 tctaaccagt tttcaagcta caaggtggtg tagctggcat cttctctact ctatcatcaa
     2341 acgataaaat ggttcctctc gtaacatttg ctcttgcctt tcacttccat ctcgatcgct
     2401 tcgtagttca ttacgatagc tgacatattt ccagtcttca ggtctaacat ctgacgccgg
     2461 atactccccg ggagcgattt tgcttcttaa tagattagac tcgagacaca ttataaattg
     2521 tcgtatttta tgaagtaata aagcactata ttgcgagcat tcctgatctg attttacata
     2581 tagcgtaaac tgagctccta tccccacacg agattgctga gaaactcgat tcatatcagt
     2641 tatcttccat ttatctatag gactgtcctc tgaaaataat aaaccagaca aaatttggaa
     2701 tgccagagga acttgctccc tagctatact aatatgaaac ttgtccccaa caaaatcccc
     2761 cttactctga ggattttcac gacatgcatg aataaacaca tctccactat ggttcacaat
     2821 ctggaatcct tggtacatga cagaaaaaga ttgacatata tcagagtaag ttggcacgtt
     2881 cgatgctact ttgaaatagt acgctgggta ttgccgtatt tggttgtaca atatattgaa
     2941 gttatttttc aatctttcat tcgcagaaag catctgcggt atttcagacc tcacaacatt
     3001 caaagaatct aaatttagtt ttagacaggg cttttttatg ggcatagaaa acgtcctcta
     3061 taaaatagat atattatctt atattgattg tttatcatct tttataaaac gatacattat
     3121 atgtaatgta tgtaagcgta agggtcctct ccgaacatgg tgggaaggct tagaacgc
//
LOCUS       DAJTQQ010000216         3158 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000216 DAJTQQ010000000
VERSION     DAJTQQ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.220"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..754)
                     /gene="ipaH9.8"
                     /locus_tag="OO309_004150"
     CDS             complement(<1..754)
                     /gene="ipaH9.8"
                     /locus_tag="OO309_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR5849058.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGPRIYFSMSDGQQNTLH"
     gene            complement(1016..1111)
                     /locus_tag="OO309_004151"
                     /pseudo
     CDS             complement(1016..1111)
                     /locus_tag="OO309_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1147..1452)
                     /gene="ccdB"
                     /locus_tag="OO309_004152"
     CDS             complement(1147..1452)
                     /gene="ccdB"
                     /locus_tag="OO309_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR5849059.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1454..1672)
                     /gene="ccdA"
                     /locus_tag="OO309_004153"
     CDS             complement(1454..1672)
                     /gene="ccdA"
                     /locus_tag="OO309_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR5849060.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2208..3158)
                     /locus_tag="OO309_004154"
                     /pseudo
     CDS             complement(2208..3158)
                     /locus_tag="OO309_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          783 a          761 c          771 g          843 t
ORIGIN      
        1 gatggagtgt attctgttgt ccgtcactca tggagaagta aatccgtggg ccgtggtagt
       61 ccggcgaaga ggttaatctt tgcagggctt gcagagcatg ggatgataat gggttatcac
      121 taatatgtat tgaacattca ttcctcagat taagaatgct ttccgggata tgacttatct
      181 gatttctatc aaaaaaatac tctctgacaa cgggattatt tccctcagaa aatgctggta
      241 gctcagtgag gaaattacgg gtcgctctga gatttttaag agcctgtggg agcgatggta
      301 aggagactat ttcgttatag ctgatattca tagtcaataa cgaatcgggt aaagctggca
      361 ggttttccag ctcattgtgt tgtacctgaa gtgactccag cgcaggaggt agcacgggca
      421 attctgataa tttattgctg gcggaatata atttttttag cgtaacaggc agttcaggta
      481 ggttagttaa ttgattatat gatacattga gcagcgttat ctgagctggt aagttgtcag
      541 gtagcgagga cagatttaaa cggtccagtc gaagttcatc ggaattattg ataagacatt
      601 ctttaagtcg ggagacagcc tcatcacgct cttcaccggg gagcgcttgt ttttcccatt
      661 tatcccatgc tgaaaagtaa tcagcatatg taccggaaat agtgttataa aaagaatttt
      721 ggggcaatga aaagttatta tttatcggta acatgatggg catccagtta aagccggtgg
      781 acaaatggct ttgttaaaag aaataagtag gagtttttat gaagttcatt ccatgaaatg
      841 aacttcagca cttttttatt ataataagga taaacaaaat tggctgtaaa aaaacgatac
      901 tgtttcagtt aaccgtgaat ggcctgtttg caccgtattg ttcttgtttt tcggttagtt
      961 aaacatcgta cttcatattt gaacggtaag cggctcgtca gaaccgtatt gatatttact
     1021 gagaggtcag atcaactttc caaggcaaca gatcgcgtac ccggtttgcc ggccagtcct
     1081 ggatatgctc aatgacgtaa cgcagccatt ttgtcaacgc cacgattttt gaccctagcc
     1141 tgacatttat attccccaga acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg
     1201 gctgagatca gccacttctt ccccgataac gaagatcggc acactggcca tatcagtggt
     1261 catcatgcgc cagctttcat ccccgacatg caccaccggg taaagctcac gggagacttt
     1321 atctgacagc aggcgggcac tggccagggg gatcaccatc cgtcgtccgg gcgtgtcaat
     1381 aatgtcactc tgtacatcca caaacagacg ataacgactc tctcttttat aggcgtaaac
     1441 cttaaactgc atttcaccag tccctgttct catcggcaaa agagccgttc atttcaataa
     1501 aacgggcgat ctcagccatc ccctgctgat tttcagcctg ccagcgttcc gcacgcagac
     1561 ggcgggcctc tttttgcatg gccgtattta ccaggcctga gatattcacg tttgcggatt
     1621 taagtaactg atagctgtca ctgtctatag tgacagtaat acgctgcttc atgataagcc
     1681 ctcaaaaaca tacttatgat atgtatatga gtatatatta atggtacaaa ccatcaatat
     1741 ccataaaaag tacaccatta attcgctata agtttttaat acaatttttt ctgctgcgat
     1801 ttttttatac tataattagc atacttataa caccgtaagt aaattacatg aaagcaggca
     1861 cagcgtgatc cgatttttct gcgtataacg attttccgga agtgtaatac ggttgcgccc
     1921 cgggccgggc acccttttct gttgttgtgt acgggcccac agagaatggt accggaggcg
     1981 ggctcagaag tactgagtcg ggtgatcgcg tcaggccgcg aagtactgac acgggtattg
     2041 gtgactggtg atgaagtact gccccgggta tgatttcagg ctgattcagg ttctgattct
     2101 ctgtcagtcc gcgtggttgc tggcctggct gatttcctgt tgtcaggcgt tcaacaaccg
     2161 actgcaggtt atagcccggt tcacgaacac ctgacggcag gtatcactca cccggtgaag
     2221 gtaaatccct caagaggcag caactctgcc agtcgctcct ccggccattc cggcagacgc
     2281 gtcaggacgt ccgtcagcca ggcatgtggc tccagaccat tacgttttgc tgtttccaga
     2341 aggctcatta tctgtgccgc ccggtttccg gccatcagtg aaccggcgaa cagccacgct
     2401 ttccgtccca taacaaccgg gcgaatggca cgctcgcaga tattgttgtc caggggcaca
     2461 gctccgtcac ccaggaagca gctcagttca tcgcgccggt tcagaatata gttaatcgcc
     2521 ttatttaatg gcccgtcggg cggacagcac ggctcctgct cttcaagcca ggcaaacagg
     2581 tcattcacta tcggccggga gtaccgttat cgccactgcc gtattttttc cacagggcgc
     2641 tcacggatga gtttctcaat gcggtaaaga cccgcgattt ttctcagctc cacgcctgca
     2701 cggggctccc tgctggcctt atacagattt gcgaagccgc gccctgcgtg actccagcaa
     2761 caggctgatg tgatgccggg gtgattatcc gccagagatt tgtacacgct gtatccgtca
     2821 ctcaccagcg tactgccaca ccagcactgc agccaggtct cagggtatct gagggcccgt
     2881 ccggtctgcg agtcaaaaca gacgaccggc ggaccgcttc tttcaccgct gacgtaagca
     2941 cacagccagc cggagcaggg tttaccgcct ttccgggtat caaggagcct cagggacgtt
     3001 tcatctgcat gaataacatc ccgtgtcaga agctcatggc gtagcagttt cgccagcggg
     3061 gccagtgcag ctccggcaac accgaccata tccgccattg tactgacggg gagctccaca
     3121 cccattcggg agaaaatgtg ctgctggcga tacagggg
//
LOCUS       DAJTQQ010000217         3059 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000217 DAJTQQ010000000
VERSION     DAJTQQ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.221"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..488
                     /locus_tag="OO309_004155"
     CDS             <1..488
                     /locus_tag="OO309_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5849061.1"
                     /translation="LKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVN
                     CPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRIDDAES
                     GNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNVTLDY
                     R"
     gene            568..1263
                     /locus_tag="OO309_004156"
     CDS             568..1263
                     /locus_tag="OO309_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5849062.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1292..>3059
                     /locus_tag="OO309_004157"
     CDS             1292..>3059
                     /locus_tag="OO309_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5849063.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          885 a          717 c          679 g          778 t
ORIGIN      
        1 ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt gctcttggtc
       61 actataatgc cgaacagttt cgttctgttg gcgatcgtag cccaaaaatt ccattcacta
      121 ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac gttgaagcgt
      181 ctttccgttt atggctggaa acgcgggata atggcaccgt ccctaacttc cctaatctgg
      241 caaaagtggg ctcttttgct ggcacagcga caacaggtgt gggtatccgt attgacgatg
      301 cggaaagcgg aaaccttatg ccactgaatg ctatggggaa tgataacacg atttatcaga
      361 tcccagcgga ctccaatggt attgtcaatg ttgacctcat cgcttactac gtatcaaccg
      421 ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta acgctggatt
      481 accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc cttcctcttt
      541 ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt gatattaacc
      601 ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt acagcgaacg
      661 cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa caaaggcgca
      721 gaaacgcctt atttactgca atcatgggta gataatatag atggtaaaag ccgtgcccca
      781 tttattataa ccccaccgct atttcgtctt gaggctagcg atgactcatc actgcgaatt
      841 attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat taatgttcgt
      901 gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac gctggtattt
      961 aaaacgcggg tcaaaatgtt ttatcgcccc gcacacctga agggacgggt aaacgatgcg
     1021 tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa cccaactgaa
     1081 tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag caaaattgaa
     1141 tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg aaagaacgtg
     1201 aaatgggctg cgatcaacga ttatggcggc agttccggga cagaaactcg tccactgcaa
     1261 taaaaaatat aaaaacacag gccatcaggg aatgccacaa cgacaccacc agggacataa
     1321 acgcacaccg aaacagttgg cgctcattat caaacgttgt ttgccgatgg tgctcactgg
     1381 cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc ccattatgct
     1441 ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa aaaaatacgc
     1501 acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg tttcacagaa
     1561 aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta cggtagaaca
     1621 actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa aagatgacga
     1681 tagcgtgatc aactcgcttg aacaaatcat tcccggtaca gctgctgaat ttgatttcaa
     1741 tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg caagaggtta
     1801 cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact actcgtttac
     1861 aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc tgaatatgca
     1921 aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga cacgcaacga
     1981 tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca aggcgttgaa
     2041 gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca gctacgcctt
     2101 tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc gcggatttgc
     2161 cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc aaaatggtta
     2221 tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc tctacccctc
     2281 ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacac aacgtcgctt
     2341 tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa aatatagcgc
     2401 gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg aatttgctga
     2461 agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc tgctcggttc
     2521 tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac tgggcgcgtt
     2581 gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt ttcatggcta
     2641 tcaatggcgt actcagtaca tcaaagatat cccggaaacc aacaccaata tcgctgttag
     2701 ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata cccgcaattg
     2761 gggctataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga caatatttga
     2821 tggggtaagt ctgtatgcct ccggttcaca gcaagactat tggggcaata acgagaaaaa
     2881 cagtaatatc tctgttgggg tttccggcca gcaatgggga attggttaca gcctgaatta
     2941 tcaatacagc cgctacactg atgaaaataa tgaccgtgca ctctctttga atctcagtat
     3001 tccgttagaa cgctggtgga tttgccccta tatttccaga catctgttat cacttaacc
//
LOCUS       DAJTQQ010000218         3002 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000218 DAJTQQ010000000
VERSION     DAJTQQ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.222"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            156..983
                     /locus_tag="OO309_004158"
     CDS             156..983
                     /locus_tag="OO309_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5849064.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1027..1776
                     /locus_tag="OO309_004159"
     CDS             1027..1776
                     /locus_tag="OO309_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR5849065.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            1773..2342
                     /locus_tag="OO309_004160"
     CDS             1773..2342
                     /locus_tag="OO309_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849066.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2466..2708
                     /locus_tag="OO309_004161"
     CDS             2466..2708
                     /locus_tag="OO309_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5849067.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            2712..2858
                     /locus_tag="OO309_004162"
     CDS             2712..2858
                     /locus_tag="OO309_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849068.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            2867..>3002
                     /gene="xisR"
                     /locus_tag="OO309_004163"
     CDS             2867..>3002
                     /gene="xisR"
                     /locus_tag="OO309_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HCR5849069.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     H"
BASE COUNT          805 a          723 c          789 g          685 t
ORIGIN      
        1 ctggcaatta tcgcatgtat ttacggatta atcgttccgg ccctcatatc aatgaaggat
       61 acggtagcag tgatttctgg ctttgctctg gcgtgtctga ccccgccctg catttatgcc
      121 atttataagg gtctttcttt ttctaaggat aaaagatgaa aaaagttatt cctgctttag
      181 cccttgttct gctgaccacc ggacttgtcg gttgcgatcg cgttgagcct ggtaatgttg
      241 gcatcaaggt aaacaaactg ggcgacgata aaggcgtcgg agaagtggtc ggtgttgggc
      301 gctactggac gggatggaac actgaggttt acatcttccc gaccttcaaa caaatgaaga
      361 cctacgatga gccattcagc ttccagatga gtgacggtac aaccatcggc tatcacatcg
      421 gcgtggccta caaagttgat ccatccaaag ttaccacagt ctttcagacc taccgcaaag
      481 gcgtggacga cattaccgac accgacctgc gccagaagat agccgacgca ctcaaccgac
      541 tggccagcaa aatgaccacc gacaaattta tcgacggcgg caagtctgag ctgctggatg
      601 cagctcttaa agacattcag gaagaaatga cgcccatcgg tattcaggta atgagcctct
      661 catatgtggg taagccggag tacccgccta ctgttatcga cagcattaat gccaaagtca
      721 cggcgaacca gaaaaccctg caatgcgagc aggaagtaaa acagcgcgaa gcggaagcca
      781 acatgttgcg cgcggaagct gccggacagg cagatgcgat tcgcacaaaa gcccaggccg
      841 aagccgatgc tattcgttta cgcggtgaag ctctgcgcca gaacccaaac gttatggagc
      901 tggaagccat caacaagtgg aacggtacac tgccgcaata catgaccagc ggtgccaaca
      961 caccatttat tcaggttaag taatacatat gcccggtatt acacgccggg ctgtctggag
     1021 ataaaaatga atactgtaac catcaacaac aaacagtttc cggtaatcga atatcgcggt
     1081 cagcgtgttg tgacgctggc gatgattgat gaagtccacc agcgaccgga aggaaccgcg
     1141 cgtgcagcat tcaaccgaaa ccgtgagcat ttcatcagcg gtgtggatta tgccgaatta
     1201 ggtgcggacg taatacgtac ggacctcccg gaaggaacat tctctaaatt tgcaccatcg
     1261 gggattgtac tttttgaatc aggttacctg atgttgacga agccattcaa tgacgccctt
     1321 gcctggcagg ttcagcgcga actggttaac agctacttcc gcactcgcac accgctaacg
     1381 gaaatcgaga tgatcgctgc gatagccgcc gacgctgttc gtcaacagaa gcgcctgaat
     1441 catgtggaag aacagctcga aacagtcaca gaagcggtgg aaaccatcaa acgcggaaat
     1501 atgcgcgccg gatatgtcgg ttaccgccag gtggtagcca aaagtggaat gagtgacacc
     1561 aagtgtcgga atctggtgaa cgcatatcaa attcctacag acacccacga attcatgact
     1621 ccggacggtc tgttgtctcg cagggctatc gtcgagcttg agccatttat ggcggcgttc
     1681 caccaaatga tgtcagaggc tgacccacgc ggcacacgct ggtatcaccc gaagatgggg
     1741 cttttccagg tgatcggatg ggaggataaa gcatgatcat ccagtcaaaa cttattcgcg
     1801 ccgctctggt gtgcgctgct aaaaacgacg ttcgttatta cctgaacggt cttcacatca
     1861 cgccgaaaca tattgaggca accaatggtt ccgtggcact gcgcatggct cacggcatcc
     1921 ggacgaagaa aaacatcatt gtccagttcg aagggggcgt cccggccaaa gccgaaacga
     1981 cagagctgat ttttagtaaa gagccgatcg ctgttcatcg cgaccagttt cagcgacgac
     2041 tgtccattac cggcattaaa ctggtggacg gttgttttcc ggatttggat cgcatcattc
     2101 cgaaaaagtt tgaccgctgt actcacccgg tgctccaggc gggttacctg agttatccag
     2161 agaagatgtt tggtcgtgag cgtaaattta ttcccgtcca gttacgtccc tccggtgacg
     2221 ggcaagcggt cagaattcag tttgattcca tcatcaactc aatgtatggc aatcctgaat
     2281 ttgttgtgat gccttgtcgt gatcatggcg atttcaacgt ggctcaggag catccggaat
     2341 gaaaatcgaa taccaggacg ccgcaggagg tgaatcaatg agctggcctg atgcaatcgt
     2401 aactctgggg atggtattcg cagtagcgtt tgttgtgtac tcgatttgtc gatggggata
     2461 accacatgtt cgctttgatt caacgcgggc agatatacac agacagagcc ggatacccag
     2521 tagaaattac tcgcagtact gagcactcag tgttctttcg gaggatggac ggacgtaccg
     2581 ggcgagtacg catcggtgaa ttcagtagcc tgttcgagca tattgaccac ctggaatatc
     2641 acaagattct ggctgaaaca gaacaagaaa agcacctgaa gaaattacgc gcaatgcaaa
     2701 ggaagtaaaa aatgaataaa gcgtttgagc tatgggtcca ccagcgttac ggcaaccact
     2761 acgacctgac gcgagatgtt gacggtttct actgtcgtga aattgtgaaa cgaatgtttg
     2821 aggtctggtg ccactgccgt gggctgagtg ttgtgtgagg taatgcatgg gcaatgtgat
     2881 tcaactggct cccaatgaat gggtttgtga aagcgttcta atcgcaatta ccgggctcaa
     2941 accaggcaca attcttcgga cccggaaaga atgctggatg gttggaagag agtatattca
     3001 cg
//
LOCUS       DAJTQQ010000219         2992 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000219 DAJTQQ010000000
VERSION     DAJTQQ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.223"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(1..1551)
                     /locus_tag="OO309_004164"
                     /pseudo
     CDS             complement(1..1551)
                     /locus_tag="OO309_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(1544..2691)
                     /gene="flhB"
                     /locus_tag="OO309_004165"
                     /pseudo
     CDS             complement(1544..2691)
                     /gene="flhB"
                     /locus_tag="OO309_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
BASE COUNT          758 a          869 c          721 g          644 t
ORIGIN      
        1 gggcgacgcg atccaacagc tgttgcgcct cctgacgacc aaacagctct gcggcatgct
       61 ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct ggcctcaacc actgtgtacc
      121 cctgaatctg cgcctgttct tttagcgcac tttcaatcca gatagcattc aggccaaatg
      181 ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc ggttccaggg ttaatcgcca
      241 gccagcgccc cggataagca tcaccactgc caatctccac gcctttcatc aaaatgcgat
      301 agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac cactggcggc agaaatccca
      361 tctcctgggc aaatttcttg cggatactgc gtatacggcc caacaactca ccatcctgct
      421 ggaaatcgac catcgggatc agtcgataac ccacttccat tcccagagaa tcttccagtt
      481 gtacatcgtt ccacgtcgct tcgacaacgg tattattctc tgccattttt accggtttgg
      541 gttcggcagg cgctttttgt tcgcgtccgc gtatccacca ggccagcccc agcaatccgg
      601 cagtgaacag caaaaatacc aggttcggca ttccaggcac caggccgagt aaaccgagca
      661 cggcggcgct taacaacata acgcttgggt tactgaaaag ctgattcacc atctgctcgc
      721 caacatcctg atcggtgctg acacgcgtaa cgatgccccc cgcggcggta gaaatcacca
      781 gcgccggaat ttgtgccacc agaccgtcgc caatggtcaa tagcgtataa ctttccgccg
      841 cgtgtcccat gctcatgcca gttgcagcac gccgaccagc aacccgccga caacgttaat
      901 gaccatgatg aggatcccgg caatggcatc gccgcgaaca aacttacttg ccccgtccat
      961 tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg cgttttttcg cctcatcttc
     1021 accaatcaat ccagcgttaa ggtcggcgtc aatcgccatc tgcttacccg gcataccatc
     1081 gagaacaaag cgcgcaccca cttctgctat acgcccggca cctttggtaa tgaccataaa
     1141 gttgatgatc acgagaatga caaacaccac gataccgata gcgaaattgc caccaacgag
     1201 gaagtgaccg aacgcttcga ccaccttccc agccgccgcc gcgccggtat gcccttccat
     1261 caaaatgata cgggttgaag ccacgttaag tgccagacgc aacaacatag taaacaacag
     1321 aatggtcgga aacgcagcaa actcaagcgt gcgctgggta aacatcgcca ccagcaacac
     1381 catgatcgac aaggcaatat tgaaggtaaa caacaggtcg agtatgaatg cgggcagtgg
     1441 cagcaccatc atcgacaaga tcaacaggat caaaatcggt ccggcaagga tctgccatta
     1501 tgtcgatttc aggtttgcgg gcaggcgcag catcgcggcc agattactca tgggtcggtt
     1561 tctcgttaat aaaatccagg gctgccggca ccggaagatg agtaggtgta cagggcgctg
     1621 tccaccagcc agacgccagc gtttcagttg ccagacccag gccagcactt ccgccaccgc
     1681 ggcgtacagt tgacccggga tttgttgacc aatctccgca tgtcgataca gcgctcgcgc
     1741 cagcggcggc gcttcaagcg tcgggacgtt attttcagcg ccaatttcac gaatgcgcag
     1801 cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg ctcattttgt tttcgtcata
     1861 ctgcaacgct accgaatagt gggtcgggtt attgacaatg acatccgctt tcggcacatc
     1921 ggccatcatc cgacgccgtg cggcagctcg ctgcatctga cagatccgtc ctttaacatg
     1981 ggggtcgcct tcgctttgtt tgaactcatc acgaatatcc tgccgcgaca ttcgcagctt
     2041 tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc accattggaa tgacaccaag
     2101 caccaccagc agtgcgcata gccctaccaa atccatcgca ttacccatgg cggtaatcgg
     2161 tgactcggcc attaagcgca tcatctgcgg ccagtgatgc cagagaaaaa accccgtcac
     2221 gctgccaacc aggatggttt tcaaaattgc cttaagcaac tccgcgctag tctgtgccga
     2281 gaatatccgt ttaatgcccg gtagcgggtt gagtttggaa aacttcggct gcaaggattt
     2341 gccgctaaat accagccccc ccagcataac cggagaaatg agcgccacca gcaccacgcc
     2401 gctaatcagc ggtagcagcg ccagcatggc ttctctgatc agcagaataa tttgcccgag
     2461 gatcagattc gggtctttga taatactgtg atcaaaatgc agcccagcgg agagcatgcc
     2521 cgacaatcga cgggccagcg acacaccgcc aaaccagata acactaacgc ccaccagcaa
     2581 aatcagcagt gaggtcagtt cacgggaacg cgggatttgc ccctcttccc gcgctttttc
     2641 tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc tcgtcagaca cgtcgccaat
     2701 cctggaaaga gtattaagcg taaatgatgc cagagcgcaa agcgttcaat ggtttaagta
     2761 aggggcaaaa caggcgggat ttagggcgtt tatgcaaaaa taggttcagt tgcagaacgt
     2821 tttgcgaacc attttccagt tctgatattt agccatttag cctatagaat cgcactccgc
     2881 ctctctgtta gtttgctaat aattaaatta ttacaaaggt gatcaaatgg aatggcaaca
     2941 attgcttaac tgcaaccgtc ggaaagataa tctaaaaaaa accaacatag ca
//
LOCUS       DAJTQQ010000220         2972 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000220 DAJTQQ010000000
VERSION     DAJTQQ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.224"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            1..799
                     /locus_tag="OO309_004166"
                     /pseudo
     CDS             1..799
                     /locus_tag="OO309_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            914..1255
                     /locus_tag="OO309_004167"
                     /pseudo
     CDS             914..1255
                     /locus_tag="OO309_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1238..2075
                     /locus_tag="OO309_004168"
                     /pseudo
     CDS             1238..2075
                     /locus_tag="OO309_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(2125..>2972)
                     /locus_tag="OO309_004169"
     CDS             complement(2125..>2972)
                     /locus_tag="OO309_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HCR5849070.1"
                     /translation="ARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRG
                     LLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRL
                     AADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPV
                     LTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNK
                     ALKRALFLSAFTALRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGTFY
                     TPQGS"
BASE COUNT          689 a          738 c          929 g          616 t
ORIGIN      
        1 tctgggcctt gcccgtagca gttattttta tcaccgggct gcactgaaag ccggtgataa
       61 atacgccacg atacgtaaga tgctgacaga tatctttaac agtaattacc agtgttatgg
      121 ctatcgtcgc ctgcatgcga tgctcaggca tgaggggggg cggctatcag aaaaggttgt
      181 acgcagactt atggtggagg aacagcttgt cgtcagtcgt aaccgtcgtc gtcgctacag
      241 ctcatattgc ggagaaatcg gaccggctcc ggataacctt atcgccagag attttaaggc
      301 ggagcaacct aatcagaaat agctgacaga tatcacggag ttccagctcc ctgcaggtaa
      361 agtctggctg tcaccagtgg tggactgctt cgatggaaaa gttgtgagct ggtctctcag
      421 tacacgccct gatgctgaac tggtcaacac tatgctggat agcgctgtcg aaacgttaaa
      481 tgctggcgaa cgaccggtga tacacagtga cagaggtggg cattatcgct ggccaggctg
      541 gctggaaaga gtgaatgcag caggtcttat tcgctcaatg tcccgtaaag gatgttcacc
      601 tgataatgct gcatgcgaag gctttttcgg cagactgaaa acggaaatgt attatgggcg
      661 taaatggtcg ggcatcacgc cagaaaagtt catgcagcaa gtggatgctt acatcagatg
      721 gtataacgag cggcgtataa aattatcgct gggtgccgtc agccccaaaa tgtaccgcca
      781 acaatgcggg ctggaatgat aaagcagtcc aggaaatcgt ccgcatcccc ccttcaccat
      841 ttggattgtc cgctccaccc aacatgttgt ttccttaagg ttcctacacc acaaaggaaa
      901 ctctcatgct gcgcagtgga tggctgacgt tgccgacagg cgggaacttc gccagttcag
      961 gcagacaccg gaacagcgct tcacgcagga acaggagcat ctgcagccgc tgctgggtac
     1021 ggacttcgat atccgtcatg tctcctggga tggctatatc gaggttggcg ggaaccgtta
     1081 cagtgtgccg gaaagcctgt gtggtcagct ggtatcaata cggatctcgc tggatgatga
     1141 actgcggatc tacagtaatg agcaacaggt agcatcgcac cgtctctgtt cggctgcata
     1201 cggctggcag accgtgccgg agcatcacgc tccactctgg cagcaggctt cggagcgcat
     1261 ggccgaatgg ctgggggaga tacagaaacg ggtaatcact gtctgtgacc gggaagcgga
     1321 tatctggcat tacctgtatt ataaagtcag ccacggacag cgtggtgcgt gctgcacaga
     1381 atcgccggct ggaagaggca cccggcaagc tcttcgaact gccggaagtc ctggcaaccg
     1441 caggaagcca cacgctgaat gtgatgcaaa aaggcgggcg ggcagcccgt caggcccgga
     1501 tgttcatcag ttacagcgaa gtcagcataa aaaatcccga caacagcggc caggcgctcc
     1561 cgctcacgta tgtctgttgc cgggagcagg cagaggacgg tgcctgctgg catctgctga
     1621 cgtcaggaaa agcggcgagt gccgccgatg cacgacgtat tgtcagccat tacgagcgac
     1681 gctggctgac agaggaatac cacaaggcgt ggaaaagtgg tggtacatgg aatcgctgcg
     1741 aatgcagacc cgggataacc ttgagcgcat ggtggttatc ctggcgttta tagcggtacg
     1801 ggtgctgggc ctgcggcagg gaggtgtcag tgaagaaaca caaaatgaca gctgtgagaa
     1861 aatactgacg ccgacagagt ggaaactact gtgggttaag ctggaaggaa agccgcttcc
     1921 ggtgcaggca ccgaccctga aatgggcctg gggcgatggc atgacagcaa acgcacaggt
     1981 cgtcccggtt ggggcgtcat gtgggatggc tggtcagact tcaggatatg gttgagggtt
     2041 acctggtgat gaagtctctt gatcaggagc tctgatcaag agacagcagc ttaggggggt
     2101 gcccctatta agttgtcaag catgttatga cccctgcggg gtataaaaag tcccgtcgcg
     2161 catcatggcg aacagaacgt cgcagcgtcg tctcgccagg gcgataagcg cctgattgtg
     2221 tcgttttccc tgactcattt tgcgggtgta gtaagccctg gagagcggat ccctgagcgc
     2281 ggtgaaggcc gacaggaaca acgcccgttt gagagcttta ttaccccgtc gcgagggatg
     2341 ctcaccgcgt attgacgagc cggatcgccg agttaccggc gcaaggccag cataagcagc
     2401 gagatgtgcg gcagaggcga aggcgcggca ggcgacctcg gtgaggagtc tggctgcggt
     2461 cctgacaccg actccgggca tactggtcag gaccgggtaa agagggtgag caagaactcg
     2521 ctgttctacc tcaagcgcca cctcgtctct ttgcttacgc agcgtgatga gctggagtgc
     2581 cagacgtggc agtactacgg cagcggcatt cgtgccggga acgacgacgg tttgttcggc
     2641 cagtgcctga gctatgtctg ctgcaaggcg tttacccaga cgaggcgcaa gtttgcagag
     2701 ctgggctgcc agcttcttct cacccagcga agcgagtttt tctggtgagg gatatcgctg
     2761 gagaagatcg agtaccgccg ggtgctcaag tctcggaccg agaacgcgct ccggtgccgg
     2821 atgtatctgg gtcagaaggc cgcggatacg gttgctggcc tgcgttgtct gtgcggcaag
     2881 atcatcatcg aagccgcaga gcatggagag ttcggcgatt tgctcgtcag ccagtttcag
     2941 cgtgcgtagc gcgtgaggca gggtacgggc ag
//
LOCUS       DAJTQQ010000221         2912 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000221 DAJTQQ010000000
VERSION     DAJTQQ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.225"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            112..1980
                     /gene="kup"
                     /locus_tag="OO309_004170"
     CDS             112..1980
                     /gene="kup"
                     /locus_tag="OO309_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR5849071.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2147..2566
                     /gene="rbsD"
                     /locus_tag="OO309_004171"
     CDS             2147..2566
                     /gene="rbsD"
                     /locus_tag="OO309_004171"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR5849072.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2574..>2912
                     /locus_tag="OO309_004172"
     CDS             2574..>2912
                     /locus_tag="OO309_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5849073.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKI"
BASE COUNT          649 a          696 c          765 g          802 t
ORIGIN      
        1 aatgtggccg catttaggag tacgattttg ccgttaatcg tgcatactgt gcgctttttt
       61 gtgggccaag ggactaagca cacatttcat atttcaacga aagactagtc tatgagcact
      121 gataataagc aatcattgcc cgcgattacc ctcgcggcga ttggagttgt ctacggcgat
      181 attggtacca gcccgttata tacacttcgt gaatgtttgt ccggccagtt tggttttggc
      241 gttgaacgcg atgccgtgtt tggcttttta tcgctgatct tctggctgct aatctttgtg
      301 gtttccatta aatatctcac cttcgtgatg cgggcagata acgccggtga gggggggatc
      361 ctgacgttga tgtcgcttgc cgggcgtaat acgtcggcgc gaaccacatc aatgctggtg
      421 attatggggc taatcggcgg cagctttttc tatggtgaag tcgtcataac acccgctatt
      481 tcggtgatgt cagccattga aggtctggaa atcgtcgccc cgcagctgga tacctggata
      541 gttcccctct caattatcgt tctcacttta ttatttatga ttcaaaagca tggcacggct
      601 atggtcggta agctgtttgc gccgatcatg ctgacctggt ttttgattct ggcaggactg
      661 gggttacgta gcattattgc taacccggaa gtgctgcatg cgctgaatcc gatgtgggcg
      721 gtgcatttct tccttgaata caaaacggtt tcttttattg cattaggggc agtggtgctg
      781 tcgattacgg ggggcgaggt gctgtatgct gatatggggc actttggtaa gttctctatt
      841 cgcctggcgt ggttcaccgt cgtattacct tccttgaccc ttaattactt cggtcaggga
      901 gcgttgttgt taaagaaccc ggaagcgatt aagaacccgt tcttcctgtt ggcaccggac
      961 tgggcgctga tcccactgct gatcatcgcc gcactggcga cggtaattgc ctcgcaggcg
     1021 gttatctctg gcgtcttctc attgacgcgt caggcggtac gtctgggata tttgtcgccg
     1081 atgcgcatta ttcacacctc cgaaatggag tcagggcaaa tctatattcc gtttgtgaac
     1141 tggatgctct atgtcgcggt cgtgattgtg attgtcagct ttgagcactc cagcaacctg
     1201 gcggcggcgt acgggattgc ggtgaccgga accatggtac tgacgtctat tctctcgact
     1261 accgtggcac gtcagaactg gcactggaat aagtattttg ttgcgctgat cctgattgct
     1321 ttcctttgtg tcgatattcc attgttcacc gctaacctcg ataaactgct ctccggcggc
     1381 tggttgccat tgagcctcgg tactgtgatg tttatcgtga tgaccacctg gaagagcgag
     1441 cgtttccgct tgctgcggcg gatgcatgaa catggtaact ctctggaagc gatgattgct
     1501 tcgctggaga aatcaccgcc tgttcgcgtg cccgggaccg cggtgtatat gtcgcgtgca
     1561 atcaacgtca ttccctttgc gctgatgcat aaccttaaac ataacaaggt attgcatgag
     1621 cgggtgattc tgttaactct gcgcaccgaa gacgcgccat atgtccataa cgtccgtcgg
     1681 gtacagattg aacaactgtc gcccactttc tggcgcgtgg tggcaagtta tggttggcga
     1741 gaaacgccaa acgtggaaga agttttccac cgctgcggtc tggaaggatt aagttgccgg
     1801 atgatggaaa cctccttctt tatgtcgcat gagtcgttga tcctcggcaa acgcccgtgg
     1861 tatttgcgtc tgcgcggcaa gctgtacttg ctgctgcaac gtaatgcgct gcgtgcacca
     1921 gatcaatttg aaatcccgcc aaacagggtt atcgaactgg gtactcaggt cgaaatctaa
     1981 cgccagacgc ctcctttctt cataaggggg cgtttttgtt ttcatggtta atcaccatgt
     2041 aaaacgtttc gaggttgatc acatttccgt aacgtcacga tggttttccc aactcagtca
     2101 ggattaagct gcggatcagc gaaacgtttc gctgatggag aaaaaaatga aaaaaggcac
     2161 cgttcttaat tctgatattt catcggtgat ctcccgtctg ggacataccg atacgctggt
     2221 ggtgtgtgat gctggtttac ccatccccaa aagtacaacg cgtatcgata tggcattaac
     2281 ccaaggtgta ccttctttta tgcaggtgct gggcgtcgtc acaaatgaaa tgcaggtcga
     2341 ggctgccatt atcgcggaag agatcaaaca acataatccg caactccacg aaacgttgct
     2401 cactcacctt gagcagctgc aaaaacacca gggaaatacc attgaaattc gttacaccac
     2461 gcatgaacaa ttcaaacaac aaaccgcaga aagtcaggcg gtaattcgca gcggagaatg
     2521 ttctccgtat gcgaatatca ttctctgtgc tggcgtgacg ttctgaggcc gtcatggaag
     2581 cattacttca gcttaaaggc atcgataaag ccttcccggg cgtaaaagcc ctctcgggcg
     2641 cagcgttaaa tgtctatccg ggccgtgtga tggcgctggt gggcgaaaac ggcgcgggta
     2701 aatccaccat gatgaaagtg cttactggta tctatactcg cgatgccggc acgcttttat
     2761 ggctggggaa agaaacgaca tttaccgggc cgaaatcttc ccaggaagcc gggattggga
     2821 ttatccatca ggaactgaac ctgatcccgc agttgaccat tgccgaaaac attttcctcg
     2881 gtcgtgagtt tgttaatcgc tttggcaaaa tt
//
LOCUS       DAJTQQ010000222         2862 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000222 DAJTQQ010000000
VERSION     DAJTQQ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.226"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            43..846
                     /gene="agaC"
                     /locus_tag="OO309_004173"
     CDS             43..846
                     /gene="agaC"
                     /locus_tag="OO309_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HCR5849074.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            836..1627
                     /gene="agaD"
                     /locus_tag="OO309_004174"
     CDS             836..1627
                     /gene="agaD"
                     /locus_tag="OO309_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HCR5849075.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1628..2383
                     /locus_tag="OO309_004175"
     CDS             1628..2383
                     /locus_tag="OO309_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HCR5849076.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
     gene            2784..>2862
                     /locus_tag="OO309_004176"
     CDS             2784..>2862
                     /locus_tag="OO309_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5849077.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGE"
BASE COUNT          733 a          647 c          678 g          804 t
ORIGIN      
        1 ctaaatctaa aatcgcctta atattggttt gaggtaataa aaatgcatga aataacccta
       61 cttcagggat tatccctagc ggcgttagtt ttcgttctgg ggattgattt ttggctggaa
      121 gccttatttt tattccgccc gataatcgtt tgtaccctaa ctggcgctat tctcggtgat
      181 attcagactg gcttaattac cggtggtctg acagagttgg ctttcgccgg attaacccct
      241 gcaggtggtg ttcagccgcc caacccgatt atggcgggtc tgatgaccac cgtcattgca
      301 tggtctacgg gcgttgatgc caaaacagca attggtcttg gcctgccgtt tagtttgtta
      361 atgcagtacg tcattctgtt cttctattcc gctttctcat tatttatgac caaagccgat
      421 aaatgcgcga aagaggcgga tacggcagcg ttttcccggc ttaactggac aacgatgctc
      481 atcgtcgctt cagcgtatgc ggtgattgct ttcctctgta cttacctggc acaaggggcg
      541 atgcaggcgc tggtgaaagc gatgcccgcc tggttgaccc acggctttga agtggctggc
      601 ggtattctgc ctgccgttgg ttttggcttg ctgctgcgcg taatgttcaa agcgcaatat
      661 atcccttacc tgatcgccgg tttcctgttt gtttgctaca tccgggtcag caacctgttg
      721 ccggttgccg tactgggcgc aggctttgcg gtgtatgagt ttttcaatgc gaaatcccgg
      781 cagcaagcgc aaccgcagcc cgttgccagt aaaaatgaag aagaggacta cagcaatggg
      841 atctgaaatc agtaaaaaag atatcacccg tctgggcttt cgttcgtcgc tgctgcaagc
      901 gagctttaac tacgaaagga tgcaggcggg cggttttacc tgggcgatgt tgccgatcct
      961 gaaaaagatt tataaggacg acaaaccggg cttaagcgcg gcgatgaaag ataacctcga
     1021 atttattaat acccacccga atctggtcgg attcctgatg gggttattaa tttcgatgga
     1081 agaaaaagga gaaaaccgcg acaccattaa aggcctcaaa gtggcactgt ttggcccaat
     1141 cgccgggatt ggcgatgcga ttttctggtt tactttgttg ccgattatgg cgggaatttg
     1201 ctcatcattt gccagccagg gaaacctgct ggggccgatt ctatttttcg ccgtttacct
     1261 gcttatcttt ttcctgcgcg tcggctggac ccacgtcggt tattcagtcg gcgtgaaggc
     1321 gatcgataaa gtgcgagaga actcgcagat gattgctcgc tcggcaacca tcctcgggat
     1381 cacggtaatc ggcgggctga tcgcttcgta tgtgcatatt aacgtggtga catcgtttgc
     1441 catcgacagt acccacagcg tcgcgctgca acaggatttc ttcgataaag tcttcccgaa
     1501 cattttaccg atggcctaca ccctgctgat gtattacttc ctgcgggtga aaaaagcgca
     1561 tccggtgctg ttaatcggcg tgacttttgt gctctctatt gtttgttccg cattcggcat
     1621 tttgtaaatg gaacgaggca ctgcttctgg cggtgcctct ttacttaagg aatttcatcc
     1681 tgtgcaaacc cttcagcaag ttgaaaacta tacggcgtta agtgaacgag ccagcgaata
     1741 tttattggct gtgatccgta gcaaaccgga tgccgtgatt tgcctggcga ccggagccac
     1801 gccattactg acgtatcatt atctggtaga aaaaatccac cagcagcagg ttgatatcag
     1861 ccagctcacc ttcgtgaagc tcgacgaatg ggtggatctg ccattaacga tgcctggcac
     1921 ctgcgaaact ttcctacaac agcatatcgt gcagccgctg gggctacgtg aagaccagct
     1981 catcagcttt cgctccgaag agataaatga gacagagtgc gaacgggtaa cgaacctgat
     2041 tgcgcgcaaa ggcggtctgg atttatgcgt tctcggattg gggaaaaacg gtcatcttgg
     2101 gctgaacgaa ccgggagaaa gcctacaacc ggcctgccat atcagtcaac ttgatgccag
     2161 aacacagcaa catgagatgt taaaaaccac gggtcgcccc gtgactcgtg ggatcacctt
     2221 aggcctgaag gatattctca atgcccgcga ggttttgtta ttggtgactg gcgaaggaaa
     2281 gcaggatgcg acagaacgtt ttctcacggc taaagtctcc accgctatcc cggcttcatt
     2341 tttatggctg cacagtaact ttatttgttt aattaatacc taaccagatg aaaaatttgc
     2401 tcaaaatttt taaattaact tattcaacag tcacgcatta tattaaataa catttgactg
     2461 ggttgaacat aacgccaata gcaaaaggag ttatgttcaa caatttaatt ttagatatac
     2521 atataacttg ttctattaat aaatcacctc gtattctgac tcggcccctg gcgcagcaaa
     2581 gtcaggcatt tatactcttt ttaacacata gaaaattcat atcaatataa taaaaacata
     2641 aaatatttgc atatacatat aatcttaaag tttagtctat ttaatgttca atgaaatatt
     2701 tctacctgta taatctttaa agatgttgaa catatattca cattaaatat gattatgtac
     2761 ttgttacaag gataaggtta taaatgaata aagttacaaa aacagctatt gtgggcttac
     2821 ttgcgctttt cgcaggtaat gccgctgcaa ccgatggtga aa
//
LOCUS       DAJTQQ010000223         2794 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000223 DAJTQQ010000000
VERSION     DAJTQQ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.227"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..476
                     /locus_tag="OO309_004177"
     CDS             <1..476
                     /locus_tag="OO309_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR5849078.1"
                     /translation="CSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFY
                     ADVPKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSF
                     ASRPVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            479..1642
                     /locus_tag="OO309_004178"
     CDS             479..1642
                     /locus_tag="OO309_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5849079.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1726..>2794
                     /locus_tag="OO309_004179"
     CDS             1726..>2794
                     /locus_tag="OO309_004179"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5849080.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLF"
BASE COUNT          609 a          674 c          808 g          703 t
ORIGIN      
        1 gttgtagcga tagtagcgat gtcgataacg acggcgacgg aacattttac gcgacagtgc
       61 aggattgtat tgatgacggt aataatgccg acatttgcgc gcgtggctgg aacaacgcca
      121 aagcggcatt ttatgccgat gttccaaaga atatgactca gcagaactgt cagtctaagt
      181 acgaaaattg ctactacgac aatgttgaac agagttggat cccggtcgtt tctggatttt
      241 tgttaagccg ggttattcgt aaagatcgcg atgagccgtt cgtctataac agcggcggat
      301 cctcatttgc ttcccgcccc gtctggcgca acacttccgg tgattactcc tggcgcttcg
      361 gttctggcaa aaaagagtct tactcttcag gcggctttac caccagaaaa gcgtcgaccg
      421 tttcgcgcgg cggctatggt cgttcttcca gcgcccgtgg gcattgggga ggctaatcat
      481 gctgagacac aacgttcctg tgcggcggga tctggaccag atcgccgcta acaacggttt
      541 cgactttcat atcatcgaca atgaaatcta ttgggacgag agtcgggctt accgttttac
      601 tctgcgccag attgaagagc agatcgaaaa accgactgcg gaactgcatc agatgtgcct
      661 tgaggtggtg gatcgcgcgg ttaaagacga agagatcctg acgcaactgg cgatcccgcc
      721 gttgtactgg gatgtgatcg ccgaaagctg gcgcgcacgc gatccttcgc tgtatggccg
      781 gatggatttt gcctggtgtg gcaatgcgcc ggtgaagctg ctggagtaca acgccgatac
      841 gccgacctcg ttgtatgagt cggcttattt ccagtggttg tggctggagg atgcccggcg
      901 cagcggcatt attccgcgtg atgccgatca gtacaacgcc attcaggaac gcctgatttc
      961 gcgctttagt gagctttaca gtcgggaact gttttatttt tgctgctgtc aggacaccga
     1021 tgaagacagg agtaccgtgc tgtacttgca ggactgcgcc cagcaggcag ggcaggagtc
     1081 gcggtttatc tacattgaag atctcggttt gggcgtcggc ggcgtactga ccgatcttga
     1141 tgataatgtc atccagcgtg catttaagct gtatccgctg gagtggatga tgcgtgacga
     1201 taacggtccg ctgctgcgca agcgtcgcga gcaatgggtg gagccgttat ggaaaagtat
     1261 cttgagtaat aaagggctaa tgccgctgct ttggcgcttc ttccctggtc atcctaacct
     1321 tcttgcatcc tggtttgagg gtgaaaaatc gcagattgcc gctggcgaaa gctatgtgcg
     1381 taaaccgatc tactcgcgcg aaggcggaaa cgtcaccatt ttcgacggtc agaataacgt
     1441 cgttgaccac gctgatggtg attacgccga tgaaccgatg atctaccagg cgtttcaacc
     1501 tctgccgcga tttggcgata gctacacact catcggtagc tggattgtcg atgatgaagc
     1561 gtgcggaatg gggatccgtg aagataacac actgatcacc aaagacacct cacgtttcgt
     1621 tccgcattac attgctggat aagaatgttt tagcaatctc tttctgtcat gaatccatgg
     1681 cagtgaccat actaatggtg actgccattg atggagggag acacagtgca ctggcaaact
     1741 cacaccattt ttaatcaacc tataccatta aataacagca acttatacct gtctgatggc
     1801 gcgctttgcg aagcggtaac gcgtgaaggt gctggctggg atagcgattt tctagccagt
     1861 attggtcagc agttaggaac ggctgaatcc cttgaactgg ggcggctggc gaatgtaaac
     1921 ccgcctgaac tgttgcgcta cgatgcccag gggcggcgtc tggacgatgt tcgtttccat
     1981 cccgcatggc acctgctgat gcaggcgtta tgtaccaatc gggtgcacaa tcttgcctgg
     2041 gaagaagacg ctcgctccgg cgcatttgtg gcgcgcgcgg cgcgttttat gttacatgcg
     2101 caggttgagg ctgggtcgtt atgtccagta acgatgacct ttgccgccac gccattgttg
     2161 ttacagatgt tacccacgcc gtttcaggac tggaccacgc cgctgctgag cgatcgctac
     2221 gattctcact tattgccagg tgggcaaaaa cgcggtttgt tgattggcat gggaatgacg
     2281 gaaaagcagg gcggttccga tgtcatgagc aacaccaccc gtgcagagcg tttggaagat
     2341 ggctcttatc ggctggtggg gcataaatgg tttttctcgg tgccgcaaag cgatgcgcat
     2401 ctggtgctgg cgcagaccgc gggcggtctg tcctgctttt ttgtgccgcg ctttttgcct
     2461 gacgggcaac gcaacgcgat tcgcctcgag cggctgaaag ataagctggg taatcgctct
     2521 aacgccagtt gcgaagtgga gtttcaggat gccattggct ggttattggg gcaggaaggg
     2581 gaaggaattc gtctgatcct gaaaatgggg gggatgacgc gttttgattg cgccctgggt
     2641 agccatgcca tgatgcgccg tgcattttcg ctggcgattt atcatgcaca tcaacgccat
     2701 gtttttggta atccattgat ccaacagccc cttatgcgtc atgtcttaag tcgcatggca
     2761 cttcagcttg aagggcaaac ggcgttgctg tttc
//
LOCUS       DAJTQQ010000224         2620 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000224 DAJTQQ010000000
VERSION     DAJTQQ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.228"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..758)
                     /locus_tag="OO309_004180"
     CDS             complement(<1..758)
                     /locus_tag="OO309_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849081.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERISQAESTLQEHE
                     TRIAQNESDIASLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGTLAPFFDTVNNKMVVFNENKTLFFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDG"
     gene            complement(758..2176)
                     /locus_tag="OO309_004181"
     CDS             complement(758..2176)
                     /locus_tag="OO309_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCR5849082.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIGSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLY
                     DDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDGINYGCEQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGCQIRIE"
     gene            complement(2177..>2620)
                     /locus_tag="OO309_004182"
     CDS             complement(2177..>2620)
                     /locus_tag="OO309_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCR5849083.1"
                     /translation="ASNATLTDVEPQSMEDGVNDLEMMMAEWLGGDVSPGINVGYIFA
                     DADVAPEPGDEHGLSNNAINAVIFNLACRIAPDYALEAPAKLITTARYGKERLVKLSA
                     MDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEPCDNGGE"
BASE COUNT          621 a          684 c          652 g          663 t
ORIGIN      
        1 ccgtctttat ccacgctgaa gaatgtagct aacaggatgt tgtcggttgt tgtcgccgca
       61 ttacgactgc tgaccagcgt atcaggaaca gagccggaaa aggttagctg cattgacctg
      121 ttggcggttc cgctgggcca cgtcccgacg atcgacagct tgaagaacaa ggttttgttc
      181 tcgttgaaca caaccatctt gttgttaacg gtgtcgaaga atggtgccaa cgtgccggat
      241 gacggcgtga gcgttttcag taggctaaca aggttggtcg gcgctgtcgg gatggttaca
      301 gatacgccag agtaaacaac ctctgacttc ttgcgagtag tggcatactc cagagcatcg
      361 atgcgcgttt catggtctga aacctgcgat tccagcgact gaactctggt atcaagcgac
      421 gcaatatcgc tttcattctg agcgattcgc gtttcatgtt cctgaagagt tgattctgcc
      481 tggctgattc gctcctcatg attaacaagc gttgcttccg cagcagaaat tcgctgctca
      541 tggtcagcga gaatcacatc ctgctcatcg ttcctgactt gtgcgtcata agcgccctgt
      601 ccggcctcgt tggccttgtt ggccacgtta ccaacatcag tgccctgtgc gataacgtaa
      661 agcagatacg actgcgagaa gatattgcgt ggaaggactg atgtgtcgag ccgtgtagcc
      721 tgaatgatta ccggcacatt gagattcgaa tccgccatta ctcaatcctt atctggcagc
      781 cagacagagt gacaggtgac ttcgtgataa cgcgcaattt gaagccgaca tttttcctga
      841 tgcgccctac tcgcttccac aaaacgcgtt tgtcgtaaac gaacggttca ttctgctcaa
      901 tcatctgctc acacccgtaa ttgatgccgt cagtggttgc agagagaaaa aggcggtcag
      961 catactgcgc aacgccagtt gacgattcaa cctcaaggtc gaacactctg gcgttatccg
     1021 ctttgaacag tggagtaaac agcaggtgtt cctgttgctt gtcgtactgg ctgctgatgt
     1081 cgaattgcaa tttcccggtc acggactcca gcttatcgcc gcacgttatt tgattgcctt
     1141 cgtaaatgaa gtcgatagcg cggtacacat cgtcatacag gcctgttttc agcacacacc
     1201 attgcggacc attggcgctt gaagatgcgt cgtacaccag tacatggcgc ggaaggtgga
     1261 taatcagcaa ctcatgcgca tcaaaccgca gcgattccat cacgccatca gccagttcat
     1321 cagcagtgta ggagcggagg attttctcaa tgctcgcgct ggcgattggt gacacctgac
     1381 cggagccgat gatgtataca gacggcgcac ctgttgccgg attgctgatg aacgcataag
     1441 aatcagcgaa tggtgttttg cagtaagtcc cggcaatacc tttctgcacc atcagcgatg
     1501 gctgtgcgac atacaaagcg gcaccaacgg tggttgcacc tgtcagggaa aaatattcaa
     1561 tcgtcgatga gccaaagcag acgatgaagt ctcgccatgt acctatgccg atgatgccgt
     1621 ccggctgaga ctcggcacga tattgtgcac tgtagcggtc aggatgcgat tcatcttcaa
     1681 ggtcagtgat aaaccatgaa tcagtgccgt cttttgacca cgcataacgc ccacgtaagc
     1741 gcgtaatgtc gcgaactgaa cctaactcat actgagtgaa tccactgtct gtaggccagt
     1801 ttgagacggt tttaaccgtg ccatcatagc gatactcgac cagttgacca ttaacgccta
     1861 ccgcctgtga tgtccgacca tgcgccattg atacgcgacc acttccggca acatcaccga
     1921 cctcgctttc tcccttatac aacttgccac cgcaaacgcg ataaacagca ttctgcgcca
     1981 tgttgtactc gacaccgcgc gatacaccat tcacatcaga acgtttggca atgcccggga
     2041 atgagcgaag atatccgctg ctgttgagta tttctttggg tgtagccagc atattcactg
     2101 gcagatagtc gatgtagtcg gcgtttcgga aatctttgcc gacacctttc ataagcggaa
     2161 gttgctgaat cggcatttat tcgcccccgt tatcgcaagg ttccttccgg tggaagtaat
     2221 tccaaccatt ccacttcgcc aactggttac cgctaccaac aggcatacgg tttggataac
     2281 cggacttaca tttagcggct tttgctctgt ccattgcaga cagtttgacg agtcgctctt
     2341 tcccgtatct ggcagtggtt ataagttttg ctggcgcttc cagcgcataa tctggagcaa
     2401 tgcggcaggc aaggttgaaa atgacggcat tgatagcgtt atttgataaa ccgtgctcat
     2461 cgcccggttc tggagcgaca tctgcatcag cgaaaatgta gccaacgttg ataccaggtg
     2521 acacatcacc gccaagccat tcagccatca tcatttcaag gtcgttgacg ccgtcttcca
     2581 tagactgcgg ttctacatcg gttaacgtgg catttgatgc
//
LOCUS       DAJTQQ010000225         2594 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000225 DAJTQQ010000000
VERSION     DAJTQQ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.229"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..893
                     /locus_tag="OO309_004183"
     CDS             <1..893
                     /locus_tag="OO309_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR5849084.1"
                     /translation="ELARVGVNPHDVAQMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQ
                     DKMQGRQVTMRGQDLDSQTAARNQAITMRGQDIQANLGQQRINLDAETNRINNENKRL
                     DRMLSAETNDLKRQEIQSRIAANNQQLQQKQQALNDGYKDGINTLTTSMFTLNDIVSS
                     PSLKSITGLRGVIPNVPGSQAADTQARLDTFKSQAYLTAVQAMRGMGALSDAEGKKLD
                     QAVGSLQNSQSEESFRRNAGVILNTLNQKRNEAVGKYVQQNGIKRVEAPQASIDYLKQ
                     HPELSIDFINRYGYLPSLGQ"
     gene            894..>2594
                     /locus_tag="OO309_004184"
     CDS             894..>2594
                     /locus_tag="OO309_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR5849085.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     D"
BASE COUNT          720 a          643 c          684 g          547 t
ORIGIN      
        1 gtgagttagc tcgtgttggc gttaatcctc atgacgtcgc tcagatgtac caacagaacc
       61 cgcggcagtt cggcgaattt gtcgatcacc tggggatgaa cagtctcggg cccgaaaaat
      121 actttgacct acaggataaa atgcagggtc gccaggttac catgcgcggt caggatctgg
      181 attcgcaaac cgccgctcgg aatcaggcaa tcacaatgcg cggacaagat atccaggcga
      241 atttaggtca gcagcgcatt aatctggacg cagaaacaaa ccgcattaac aacgaaaata
      301 agcgccttga ccggatgcta tcagcagaaa ctaacgacct gaagcgccag gaaatacaga
      361 gccgcatagc agccaacaac cagcagttgc agcagaagca gcaagcgcta aatgatggct
      421 acaaagacgg catcaacacc ctcacaacca gcatgttcac tctgaacgat atcgttagtt
      481 ctccttcact taagagcatt acaggcttac gtggagtaat ccccaacgtt ccaggctcac
      541 aggctgcaga cactcaggca cgacttgata cctttaaatc acaagcatac ctgacagcgg
      601 ttcaggccat gcgaggcatg ggcgcacttt ctgatgccga gggcaaaaag ctcgaccagg
      661 ctgttggttc gctgcagaac tcgcagagcg aggagtcctt tcgtcgcaac gctggcgtca
      721 tcctgaacac gctcaaccag aagcgtaatg aggcggttgg taagtacgtt cagcaaaacg
      781 gtatcaagcg agtggaagcg cctcaggctt ctatagatta cctgaagcag caccccgagc
      841 tgtcaatcga cttcattaat cgctacggat atcttccatc tttggggcag taaatggcta
      901 attaccgtga tttgttagag caggctggcg cacgttacgg tgtgccagaa gggttgatga
      961 ctgcactggg tgccaaggag tcttcttaca accctgccgc agtaagctcc gccggggctg
     1021 taggattgac tcaggtcatg cctgggacat ggcgtgatat gggttatacc gatgagcaaa
     1081 tgcaaaaccc cgaatatcag gctgacgctg gcgcgcgcta tctggcaaag atgtaccagc
     1141 agtttggtaa ctggcgtgac tctcttcagg cttatcacga cggtcccggc aacgttatga
     1201 aggcaaagcg tggtgaatat acgccaggac ctgaaggccg cggttacgtt gatgatcgct
     1261 ttgctcaatg ggctggtgac ccggtgacag actcaacagt cgaacagcgc gccacctctg
     1321 caaaggtaca tcctcagcaa gaccctaaca acccgtttgc acaactggaa gtacagtcat
     1381 ccgaacaagt atcggcatca ggtgtgcagt cagacccaaa taatccattt gctcagattg
     1441 agcagcaggc agccagtcag cagccacctc aacccgtaag ttctgtcgca ccgaaacctg
     1501 ttcagcaaca aacagttaat caggccaata atgcaccaac acgggaagaa ccatcattga
     1561 tgcaacaagc tggcgattgg ctcactggtg gtcaaagtgc agggcaaatt gcagaacagg
     1621 ctggtcgtgg tctggtaaac ataccatttg acgtattgca gggtggcgca agtctgatta
     1681 atgcaatcag tcaggggctt ggggggccaa aagtattgga tgatgtttat cgtccagtag
     1741 acagaccgac agacccctac gcgcaagctg gagagtcaat aggcggttat cttgttcctg
     1801 gagcaggtgt agctggaaac atggtcattg gttctctcgc tgacgcggcg aatcaacggg
     1861 gtgattttgc cgaaaatgcc gctattaatg ccggacttag cattgctacg catggcctga
     1921 taaatggcgt tacccgtggt gttcgtggtg catcaaatat aattagtggc aataaaacat
     1981 ctgcacagag agcgaccact gcgccaacag aaacatcacc attctccggt gatgccgctg
     2041 cagcaacaaa tcctgcggtt catgccgcag aggcaagagt agcacaaggt gtaccaatga
     2101 cgcctgcgac gaagaaccca gaggaagtcg ttcgcacagt agcagcacaa aaaaggccaa
     2161 atctcgcttc atcgcttgat gaactagata tcaatcctca ggctgaagtt ctggagtctg
     2221 ctgaaaggct taatgttgat tcattactcc cttcacactt ttccgggaac gagcaataca
     2281 aggcagttga gcaagcaatc aagtcccgtg cgggttctgc tctacaggtg caggaaaatg
     2341 aagcaatcag gcagctagca cagggcgcgg gtgagataat tgatcgcgtt tccggtgcaa
     2401 aagatgctct tggtatgagc gacaagttta ttgatacggt caatggaaga atgtctgcgc
     2461 tgatgaaacg aagcgaccag ctttatcgca atgttgaaaa ggcgatgcct gcaggtgcaa
     2521 aaattgatgc gccatcaaca aggtcaatgc tcaaacaggt ggcagaagat cttggcggga
     2581 tgaaaaacct tgac
//
LOCUS       DAJTQQ010000226         2563 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000226 DAJTQQ010000000
VERSION     DAJTQQ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.230"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(64..822)
                     /locus_tag="OO309_004185"
     CDS             complement(64..822)
                     /locus_tag="OO309_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR5849086.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPGEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="OO309_004186"
     CDS             complement(830..1933)
                     /locus_tag="OO309_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR5849087.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..>2563)
                     /locus_tag="OO309_004187"
     CDS             complement(1943..>2563)
                     /locus_tag="OO309_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5849088.1"
                     /translation="AVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLPLLA
                     QWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQAVPY
                     GQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDMVSL
                     FAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          706 a          683 c          599 g          575 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcacca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat aacggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agc
//
LOCUS       DAJTQQ010000227         2562 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000227 DAJTQQ010000000
VERSION     DAJTQQ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.231"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            133..562
                     /locus_tag="OO309_004188"
                     /pseudo
     CDS             133..562
                     /locus_tag="OO309_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            749..986
                     /locus_tag="OO309_004189"
                     /pseudo
     CDS             749..986
                     /locus_tag="OO309_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1407..1985
                     /locus_tag="OO309_004190"
     CDS             1407..1985
                     /locus_tag="OO309_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849089.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(2094..2352)
                     /locus_tag="OO309_004191"
                     /pseudo
     CDS             complement(2094..2352)
                     /locus_tag="OO309_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2336..2562
                     /locus_tag="OO309_004192"
                     /pseudo
     CDS             2336..2562
                     /locus_tag="OO309_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          776 a          471 c          660 g          655 t
ORIGIN      
        1 tcctgccggt atcaacgccg acggcatgta ttgccctgaa tgcggtacgg ttcactggcc
       61 ggatggcgtt attccgcctt tctgatgctt ccccgttttg ccgatatttt tcagcaggga
      121 aaccgatggc ttaactggct ggagaagcag ccggtgcaga tgagccgcct ggaacattat
      181 gccggtcagg atgaaattgg cctgcgttac aacagccacc ggacaaaacg agaagaaaac
      241 cttgtgatga gtggcgacga gtttatggaa aggttctcct ggcatgtggc ggataagggg
      301 ttccggatgg tgatacgggg tcctgagtcc ggtgaagcgg cgattactgg aagatgtggt
      361 gtacgccata acggagacag tgagaaaaac ggcgaggcaa atcacaagga gagggatgta
      421 tcagcagtga ctgaaggttg acccgctgaa gtgcgagctg cgcggaggtc agatgcggtt
      481 tacggggctg aagaggggtt accgtctggc agagcaggtt atgatgcatg agccgctggc
      541 tcgaatacgg tggtgcggct gagagtcgca gaggggaagt tgcgtccatg ttgaggggaa
      601 cggagcaaaa aaccatcaga gatacattga atcaatcaat gtaagcaata ttaagtgtct
      661 ataccgatta cttgatgatc aggttgtgtt gaataaacgc gcattcttcg gtgctttttt
      721 aatcttttgg ggatttaaat tcctatcgat gaactgtcga tatgaaaaag cgagcatcat
      781 actgacgtca aacaaagggg tcgcagactg gggagaaatg ttcggagatc acgtgctggc
      841 aacagcgatc ctagatcggc tgctacacgc tgaatatcaa aggagagagt taccggttaa
      901 aagagaaacg taaagctgga gtgctgacca aaaacacaac gccaatcagt gatgatgaaa
      961 tggtggaaaa cggacagcat cagtaacgaa agtatcttag caggcatgaa aatcgtccgc
     1021 atcccctaaa cggtcaaaat gcttctgccg tgaaggaagg agaatgccat ctcatcactt
     1081 atatctggcg aatgaaacac agatggtcgg ccttgccccc aaacacacgg cggtgcaaaa
     1141 tggaatggca gaaaacttcg tcaaaacgat gaaatatgat taaatcagtg ttatgttcga
     1201 acctgacgtg ttaacagcag taaagtagtc tcgcgaaggt gttcaaacat tataatgaat
     1261 ggcatcctca tagtgcgcag ggttatcgct tgctgcggaa atatctgaag cgacgtacca
     1321 gtaatgggga aaatgatagc tggtttctga aaatatagag gaaaatgtga tatatatatc
     1381 ctgttaatat ttaaggagaa tataaaatga aagtctcgtt taagtcttta ggctatattt
     1441 ttcatgatat atataataaa aaacacacta tcgatgagtt caacgatgtg gtaagaaaag
     1501 cagttttatc aggtaaaatt aacgagctga atgcatgtca taaagtggca atttttcttg
     1561 ccgaaaaaga caatgagata acaaagaaag acaaagccaa gataatagat acattaactg
     1621 aaaactattc cattgaattt caacaattaa tgaatattag tgagcgcacg ttaaattcat
     1681 ctttatatat tactcctggt gaaagtggtt ttgtcagttt tgttaatcgt gaaggaaaaa
     1741 tatgtcatac tgcatatgta aaatcatcag ataatagcat gacatattac catgcaaatg
     1801 gtagcagcat tgacaaatat attacggata tgtgcggcct tatttgtatg aggcatattg
     1861 agtctactgg cattattttt tatatgcttg atgaaaaagt tttatctgcc attgctgaat
     1921 ttatgaatga aaaaggatgg cgggctgcgt tttgttcagc aaagaactta tataaatgtg
     1981 tttaatttta gaaaaactat ttggtaaaat ccaacaagag tcgagaggtt cgcgttgctt
     2041 cagcagggcc tcagcctgtt caaccagact cagttgcagg tcgtgacgca gatttagtgg
     2101 tggtggttga ttgtcagata ccagccgatg atcttgtagt gcatatcatc agactttgag
     2161 aagcatatgg ttttgcgggt aagccgctta agatgtgtcc gcaggttcag attatgtcgc
     2221 tctatccgct gagtgcattt cttgcttata acatgactgg ttgaggacaa taaagttcgg
     2281 tataccggct caggcatcgg tcatgtagaa ggcaatgcta aactggctca gcaaactaag
     2341 agcagcgcgc atccacttac ccaatgcggt tgagaaaata gccttcgatc gctttcatgt
     2401 ggcgaaacaa ccgggtgagg tggtggataa aacccgccag aatgaacatc cgcatcttcc
     2461 tgttgaaagt cgtcgtcagg caaagggaac tcgcttcctg tggcagcaca gcgataaatg
     2521 gatgaccgaa tccaggcagg aaaagctgat atggctgcgt gc
//
LOCUS       DAJTQQ010000228         2371 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000228 DAJTQQ010000000
VERSION     DAJTQQ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.233"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..545)
                     /locus_tag="OO309_004193"
     CDS             complement(<1..545)
                     /locus_tag="OO309_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849090.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDG"
     gene            complement(1207..2196)
                     /locus_tag="OO309_004194"
     CDS             complement(1207..2196)
                     /locus_tag="OO309_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849091.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
BASE COUNT          640 a          541 c          566 g          624 t
ORIGIN      
        1 attccatcca gtcgttttgg gttgctaatg atgtcctgca tccgcttaat ctggtcgggc
       61 gtagacatat aaccccacag ggtgagcaga accttttttg cggctacact atttccttgg
      121 tctgatacta atgccatata gccccataaa ctatccctca gccagacagc atcatagtgg
      181 gtttctgccg ctgttttgtc atcttcgaaa gtagaagcaa gaataaacgt tccccagggt
      241 ttctccgtca acgttaactt tcgtgtgttt tctgttaatg ttagcaatcc ttgagtttcg
      301 tctttggtat agtgactctt tatttcattt tttaatttgg ctgagtgaat cagaagacga
      361 taattaggat acttatctgg atttaacgat aaataacttt tatctgttgc tgcccccctg
      421 gaattaagaa tgttaaatac agattcgctt gtcacttctg cctctgtcgc ctggcatgtg
      481 ccgcacgaaa atataccagc gagcagaaga gttaatgcag atttcccttt cataaaataa
      541 tccatcaatc gtttgttaaa agcgtagata accacttatt caacaacttg aaaataaggt
      601 attttacctg agttgttaca aaaggattgc attgcgtaaa cgctttttat ttacaaaaaa
      661 atggggaagt gttacagcga ttgcgaaaag cctgaagtga ttgaattaag ataatataga
      721 aaatcagata cagcaaaaat ggcagattga tcaggtgaag ataaattgga gcgggcgaag
      781 ggagcctacg acaaatcgaa gacaaccagc taacctattg tttcttaaag acaggtgaat
      841 ccagttggtt gtagtgatag tcacacattt agagatagaa ggtcaagact tgaagcacgt
      901 aatattccct ctaaaggaat agaactcacg gtctgactac ccggcccttt tggtctggct
      961 caggtcaggc tatgggggct tttggctcgc tctttatatt agatgaggtc tcacattttt
     1021 cagtcaaaac tcatcgtcta atagcccata gccagaccac cagtcccagc tttagcagga
     1081 agaccacgag atagactttg tgtaggtcca tttggccctc ctactttctg tattcacaaa
     1141 ctagattact agtacaaaat aaaggccaga catccagttc ggaagtctga ccaatagtcg
     1201 ttattgttag ttagcttctt tagtgaagct caggcgtttg actacaggca ggacagcagt
     1261 agccgagcca gtacccatct tgatggtcgt agcagcttca gctacaacaa acaccttgcc
     1321 gagaatctca aggtcgtgag actgggagtc tccatcacgt gaaaggacca cagtaatgct
     1381 ctcgccatcg tgacggaagc aaccgaagct cagagccgta aaacgcttag ccatcatgaa
     1441 cacgccacgg tcttcaccgt tgtaataggc gacgtcagtc cccagcatat cgacgatagc
     1501 gtcttcacca ccgaagccct gcttagcagc cagacgggag agagccttga ggtgtgactc
     1561 atgggcgata atgccgtagt cagaaacctc agaaccgaaa gaagactccg tgttctcgta
     1621 cagggcatcc atgatagcgt cagccttagc ggtcaggcta ccagtcaggg ccggaagttc
     1681 tacggtcttc agcagctttg aggactccag cacgtcaacc atcttagagt taatggtacg
     1741 aatcacgtta tcgcagatgt tcagtgcgtc ctgctggagc gtcccctgag tgaaaccagt
     1801 ggtcgacttc tggtcccaaa gctttgtttg gtgagacacg aggatcgcct gttcaatcgg
     1861 ctcaacggtg atttcagtgc cagacggagc ggtcacaact tcaggtcgct taccaccacg
     1921 gacagggacc agcacagagc ggtcagccag agtgtgagca atgattggag tcggtaagct
     1981 gttacgggtc ggcaggactt ctacagggaa cgggatgtta tcttgagcgt ctttaagtac
     2041 accgttcagc atagccttaa cagtcttagc taactggagg gcatagacct gagggtcggc
     2101 gttagtcggc agaccttcat tgacctgaat gttgtcggta tcggtgatgc gtactggctc
     2161 aatgccaaac tggaaagcgt ttttagtcat attcattata attaatctcc ttgagtcaaa
     2221 tacttgaagt ccctaacagg cagtcaggga ccgtgtgttc tcagatgagg gacgcttgct
     2281 tacaagctgt ccatccagtt accgcctgac tcaaggtcag cgacgatagc ggcacgtgag
     2341 cgaccagcgg aatgagtagc gctgcggagg t
//
LOCUS       DAJTQQ010000229         2266 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000229 DAJTQQ010000000
VERSION     DAJTQQ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.234"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..593
                     /gene="lsrF"
                     /locus_tag="OO309_004195"
     CDS             <1..593
                     /gene="lsrF"
                     /locus_tag="OO309_004195"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCR5849092.1"
                     /translation="VPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVA
                     AQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEM
                     GAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGVDMGRN
                     IFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            617..907
                     /gene="lsrG"
                     /locus_tag="OO309_004196"
     CDS             617..907
                     /gene="lsrG"
                     /locus_tag="OO309_004196"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR5849093.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            946..1704
                     /gene="tam"
                     /locus_tag="OO309_004197"
     CDS             946..1704
                     /gene="tam"
                     /locus_tag="OO309_004197"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR5849094.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1708..>2266)
                     /locus_tag="OO309_004198"
     CDS             complement(1708..>2266)
                     /locus_tag="OO309_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849095.1"
                     /translation="FAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRILLIM
                     GEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTVYLF
                     CIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICNAIE
                     IDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          582 a          527 c          617 g          540 t
ORIGIN      
        1 tagttccccc ggcgaccaat aagccggtgg tactgcgggc gtcaggtgcg aactctattc
       61 tggcggaatt aagtaatgaa gccgtggcgt tatcgatgga tgatgccgtg cgcctgaaca
      121 gttgcgcggt ggcggcgcag gtttatatcg gcagcgaata tgaacatcag tcgatcaaaa
      181 atattattca gctggtcgat gccggaatga aagtgggaat gccgaccatg gccgtgactg
      241 gcgtgggcaa agatatggtg cgcgatcagc gttatttctc gctcgcgact cgaatcgccg
      301 ctgaaatggg ggcgcaaatt atcaaaacct attatgtcga aaaaggtttt gaacggattg
      361 ttgccggatg tccggtaccc attgttattg ctggcggtaa aaaattaccg gagcgcgaga
      421 cgctggaaat gtgctggcag gctatcgatc agggcgcttc tggtgtggat atggggcgta
      481 atattttcca gtctgaccat ccggtggcga tgatgaaagc cgtacaggcg gtggttcacc
      541 ataacgaaac ggctgatcgg gcatatgaac tctatctgag tgaaaaacag taactgcgga
      601 tttaaggaga agaattatgc acgtcacact ggttgaaatt aacgttcatg aagacaaggt
      661 tgacgagttt atcgaagttt ttcgccagaa ccacctgggc tctgtacagg aagaaggtaa
      721 tttgcgcttc gatgtcttac aggacccgga agtgaattcg cgcttttata tttacgaagc
      781 ctataaagat gaagacacag tggcattcca taaaaccacg cctcactaca aaacctgtgt
      841 cgcgaaactg gaatctttaa tgaccgggcc gcgtaaaaaa cgtctgttca atgggttgat
      901 gccgtgaggc tacaattggg gtaacgcgct gacgggagta aaaaaatgtc tgactggaac
      961 ccctctttat atctgcactt ttccgctgaa cgatcgcgtc cggcggtgga gctgcttgcc
     1021 agagtgccgc tggagaatgt cgaatatgtt gccgatcttg gctgtggccc aggtaacagc
     1081 accgccctgc tgcatcaacg ttggcctgcg gccaggataa caggcatcga ctcgtctccg
     1141 gcgatgattg ctgaagcgcg cagtgctttg ccagactgcc agttcgtgga agcagacatc
     1201 cgcaactggc aaccggaaca ggcactcgat ctgatttttg ctaatgcctc actgcaatgg
     1261 ctgcccgacc actacgaatt gtttcctcac ttggtttctt tacttaatcc gcagggcgtg
     1321 ctggcagtac agatgccaga taactggctg gagccgaccc atgtgctcat gcgcgaagtt
     1381 gcttgggaac aaaactaccc ggatcgcggg cgtgagccgt tggctggcgt tcatgcttac
     1441 tacgatattt tgagcgaagc cggatgtgag gtcgatatct ggcgaaccac ttactatcac
     1501 cagatgccgt cgcaccaggc gattatcgat tgggtgaccg ccactggatt acgtccgtgg
     1561 ttacaggatc tgaccgagag cgaacagcag ctttttctta cgcgctacca tcagatgctg
     1621 gaagagcagt atccactgca agagaacgga cagatactgc tggcatttcc gcgtctgttt
     1681 attgttgccc gccgtacgga gtaattatca cgtcagctgg taatgacgat cgggaagaat
     1741 ttttgctgga atttctgctt catcgttcat ctgtaacagg tcaatttcaa tagcgttgca
     1801 gatggcatcc agcggtaaat cattgttttc agtaccgaac ggatcttcca gttcttccgc
     1861 cagacaatcc agcgaaataa aagtgtagga aatcagcaca gagataaaag gcgtcatgta
     1921 atgcaggtcc acgaccagcg cgaacggcag catgatacaa aacagataaa cggtacgatg
     1981 caaaatcagg gtgtaggcaa agggaattgg cgtgtaggca atgcgctcac agcctgccag
     2041 aaccgctgaa atatcattaa gccgatcgtt gaggctaata aacaggatat ctgaaagctg
     2101 tccattgcgg cgctgaaccg ccaaccattc tcccattatt aacaagatac ggttagctgg
     2161 agagttcgaa gccagtacac gctgaagatc tccagtcttg agataatgag ccagcacttc
     2221 cgcctgtggc tgtttgcgta atgtcatgcg taaacagtgg gcgaag
//
LOCUS       DAJTQQ010000230         2257 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000230 DAJTQQ010000000
VERSION     DAJTQQ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.235"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            671..2179
                     /gene="ltrA"
                     /locus_tag="OO309_004199"
     CDS             671..2179
                     /gene="ltrA"
                     /locus_tag="OO309_004199"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5849096.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          574 a          557 c          668 g          458 t
ORIGIN      
        1 tgtgcccgga gttcagggcg ggcatggatg cttaaatgaa ccgcgagtct gtctggaata
       61 ttgaaccggt aactcacgat gagaaaccca acaatcccac cgggtgtgac ggtggagaac
      121 ctgagcggca gtgacctgcg gcatgcccgc agggtgatgt aacccgctga caacggggat
      181 tgaggcgaga tcactaagcc gagatgatcc tcaaggttaa gtactgaaag gctgaagaac
      241 atgaacccgt taatccgcct ctgtgggttg aaaacgtcac cacggcctac gtgatctgac
      301 aggccgtgca ggaggaactg gcagtgatac gtaagcactg ccggtcgaag gtgttttgac
      361 atgtatgcga aacaccgggg cagcagcgtc tatcacactt gcgttgctga cttctgccaa
      421 cttgcggcaa gcaaggataa agagtgcgac gggcagcctc ctcagtatgc ctgagtccag
      481 gcaggtaaac cggggaaggt cagcgacgga tgttaagggg gcatggctcc gatgacgcgc
      541 tggctggcgg agcttccgta gtagtccgcg atggggaaag cccattacat ggcgaaggga
      601 agcagtttga atgtgtttgc gacgtgaatt aactgaccta acgaggtgaa gacctttgat
      661 aatcagcgaa atgcaacgca agcttgccac atgggcagcc accgatccgt ccctacggat
      721 tcaacggctg ctgcgtctga taacacaacc agaatggctg gctgaagcgg cgcggatcac
      781 gctttcatca aagggggccc atacccccgg cgttgatggc gtgaacaaaa caatgctaca
      841 ggccagactg gctgttgagc tgcaaatcct cagggatgaa ttactctcag gccactacca
      901 gcccttgccc gccagacggg tttacatccc taaaagcaac ggcaaactgc gaccactggg
      961 tatccccgcg ttgcgggatc gtattgttca gcgggccatg ctgatggcga tggagccgat
     1021 atgggagagt gattttcata cgctctcata tggcttccgg cctgagcgca gtgtccacca
     1081 cgcgatccgc acggtgaaat tacagctcac agactgtggt gaaacccggg gacgctgggt
     1141 gattgaaggc gacctgtcca gttacttcga caccgtacat catcgactgc tgatgaaggc
     1201 tgtacgccgc aggatcagtg acgcacgttt catgactctg ctgtggaaaa ccatcaaggc
     1261 gggacatatc gatgtcggtc tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt
     1321 tatatcgccg ctattatcga acatcatgct gaatgagttc gatcaatacc tgcatgagcg
     1381 ctacctgagc gggaaagcca gaaaagatcg gtggtactgg aataacagta tccaacgggg
     1441 ccgaagtacg gcggtcagag aaaactggca gtggaaaccc gcggtggcgt actgccgcta
     1501 tgccgatgat tttgtcctca tcgtcaaagg caccaaagca caggcggaag ccatcaggga
     1561 ggagtgtcgg ggtgtgctcg aaggcagtct gaaactcagg ctgaacatgg ataagactaa
     1621 aatcacccat gttaatgacg gctttatctt tctggggcac aggatcattc gcaaacgcag
     1681 tcgttatggc gagatgcgag tggtctcaac gatcccgcag gagaaagcca gaaacttcgc
     1741 cgcatcgctg acagcactgt tatcaggcaa ctacagtgaa agcaaagtcg atatggctga
     1801 acaactcaac cgaaaactga aaggctgggc catgttctat cagttcgttg attttaaggc
     1861 caaagtcttc agttatatcg accgtgtcgt gttctggaag ctggctcact ggctggcccg
     1921 caaataccgt acaggtatcg cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg
     1981 tcagagcaaa acgtgggttt tatttggtaa aaccaatcac ggcaagctca gcggcgaaat
     2041 actgtaccgg ttggtggggc aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa
     2101 tccctatctg aggacggaga ccagaaacac gtatacatcg cgctttacag aagtggcaat
     2161 ggcgttcgcc agcatttaaa tggagagccg gatgcgctga aaggtgcacg tccggttcgg
     2221 ggaggagagg cagggaaata gtccgactac gccctgc
//
LOCUS       DAJTQQ010000231         2255 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000231 DAJTQQ010000000
VERSION     DAJTQQ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.236"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            114..1718
                     /locus_tag="OO309_004200"
     CDS             114..1718
                     /locus_tag="OO309_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5849097.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          646 a          490 c          536 g          583 t
ORIGIN      
        1 gacctttctt aaagtactcc aacctactga atcctaaggg atacggcttt aagcctcctt
       61 gcagagttac ttactgtaac ccactgtgta accctctcag ggcatgcaga catatgattc
      121 agctatccag tagtcacaaa cttccagcag tctactatct ctaccagcgt aacggcgtct
      181 actacttccg tcttagggtc agacaatcaa acaatgatag aatgacctca atatcattac
      241 ggaccaagga tagacgcacc gctatggctt actcaaggca cataaaggca gcactcaagg
      301 caatacacgc agacagaccc aacgctacct atgaggaaat gagggaacac cttaaggaca
      361 tcgctgagtg tgaactgtct atgggccgtt cagatctctt tgagcctgac atgagggata
      421 tctaccggga ccaatacgga gaacttggtg agagccttac cgatgctctg gctagtgagc
      481 cgctaagcat tgaccagcat cgttacatca atgaggccct gaaggtgcta aaagcctgta
      541 tgaggcgtat cgaagcggga gacagtcagc ctctgataga ttacgtcgat ctttttaatg
      601 atattgatag gcaagataac caagcagact ctgtgtccct atctgttaat gctccagaag
      661 taaaaccaga agttacgcct agcattacca tagcgagcct ctttgaacaa tacgaagcgg
      721 agaactatca gaactggaag cctgcaacct taagggaaaa caaggcgtca cacgctgcgt
      781 taatcgaaat attcgaccat ctgggtctca atgcagacgc taacagggct gacatgctta
      841 gggtccgtga tgtgctccag cagttaccta ggaaccgtaa gcagcgcttc aaggatgtcc
      901 cacttgctga cctgttgagc cgtgaagata agacagattg tctggatgtc gttaccatca
      961 acaacaagta tcttattaag atggctgctg tctttaggtg ggctgtgcgc aatgacttga
     1021 ttaaaaagaa catgactgag ggtctggagc ttaaagtccc tcagcgtaaa gcctcagggg
     1081 cccgtaacgc cttctctacg gagcaggtgg ggcaactact ggtcgcagct aaggcatact
     1141 ctcagaagac ctctggtaag ccgtatcatt attatgttac cgccttggct gctatcactg
     1201 gggcgagact caatgaaata gcccaactac aggtcaaaga cgtcaggacc actgaggcgg
     1261 gaaccgttta tattcatatc aatgaagacg atagtagtct acccggtaag agcatcaaga
     1321 acgctcatag tgaccgctgt gtgcctctgg tcgatggggc ctatggtttt atattggctg
     1381 actttatggc gcttgtagag acccgtagag gcgctgacgg tgatgacgct atggtgttcg
     1441 atggtctgcg tctcatgaag aacggttacg gtgagcaggt gagtaaatgg tttaatcgga
     1501 cgttgttgcc taaagtcctc gtagaccgta gcggcttagc gtttcactcg tttagacata
     1561 cagttgcggc ccagttaaaa caacatgggg tcgagttggc gtatgcacag gcgatcatgg
     1621 ggcatagttc agggtctatt acgtacgacc gttatgctaa agaggtcgag gttgataggc
     1681 tggtcaatgt gatggctgat gtttataagg agacctagag gagaccatag tctaggatga
     1741 cacttactac agacctgtgt ggtcatgttt ttgactcata gttttctcta cttgtcaagg
     1801 gataattaat acgactcact atagggagaa gggacacaaa aagttttctc ttctaggatg
     1861 acacttacta cagacctgtg tggtcatgtt tttgactcat ggttttctct acttgtcaag
     1921 gataattaat acgactcact atagggagaa gggacacaaa aagttttctc ttctaggaca
     1981 actaaccgta acttatcgca tcctttcagg ggcgggatga ccaattacag acaccaagcg
     2041 ttctacttaa agtgaatgct tcctgtgtgc tctgggtcag tcgataagtg aatagcaata
     2101 agacacaatc atcttaatta tggtctctag tccctacagg ggcttaactt aaagttaact
     2161 taaagacaca attaaagatt aacttatagt attacttaaa gttaacttaa agacacaatt
     2221 agagattaac tttaagtatt aaataaaaga tttaa
//
LOCUS       DAJTQQ010000232         2162 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000232 DAJTQQ010000000
VERSION     DAJTQQ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.237"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..>2162
                     /locus_tag="OO309_004201"
     CDS             <1..>2162
                     /locus_tag="OO309_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5849098.1"
                     /translation="VYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFG
                     IKVDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPT
                     RWDEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSD
                     GRAEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNG
                     FAPVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGS
                     EQRFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGM
                     QAAENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDT
                     SLRLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLT
                     LSRQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYM
                     LSLSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDE
                     VGGNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVK
                     APGAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMV
                     PTRGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAA"
BASE COUNT          571 a          474 c          544 g          573 t
ORIGIN      
        1 gtgtttatag cgttgatatt tttatcaacg gtaaaatgtt tgaacgtcag gatgtgacat
       61 ttgttcagga taatccagat gctgatctgc acgcttgctt tattgccatt aaaaaaacac
      121 tgtcctcctt tggcataaaa gttgatgcgc tcaaatcgtt caatgatgtg gatgagacgg
      181 tttgcctcga tcctgctcca cgtattgaag gctcatcctg gcagtttgac agtgataaat
      241 tgcagctgaa tatatccatt caccaaatct acatggacgc gatggcttat gattacatca
      301 gccccacgcg ttgggatgag gggattaatg cgctcaccat caactacgat ttttctggtt
      361 cacatacact acgttcagat tatggttcac aagagacaga taccagttat ctcaatctgc
      421 gcaatggact gaatattgga ccgtggcggc tacgtaatta cagtacttta aacaccagcg
      481 atggccgtgc ggaatacaac tccattagta cctggataca gcgcgatatt gccgcgttaa
      541 gaagccagat tatgattggt gatacgtgga cggcgagcga tattttcgac agtacgcaaa
      601 ttcgcggcgc gcgtttgtat actgataacg atatgctacc cgccagccag aatggctttg
      661 ctcctgtggt tcgtgggatt gcaaagtcca acgccaccgt catcattcgg cagaatggct
      721 acgtgattta tcagtcagcc gttccacaag gtgcttttga gatcaccgat ctcaacaccg
      781 caagtacagg tggcgatttg gacgtaacca tcaaagaaga agacggtagc gaacaacgat
      841 tcacccaacc ttatgcttca ttggcgattc ttaaacgtga aggtctgaca gatgttgatg
      901 tcagcgtggg tgaattgcgc gatgaagacg gatttacacc ggacgtcctt caggcgcaaa
      961 tacttcatgg tttttcccac gggatcactt tatatggagg tatgcaggct gctgaaaatt
     1021 atggttctgc agctctgggt gtcggtaaag atcttggcgc tttgggcgca atttctttcg
     1081 atgtgacaca tgctcgtgcg aattttagcc atgatgatac agaaacgggt cagtcatatc
     1141 gctttctcta ttcaaaacta tttgacgaca cagacactag cttgcgcctg gttggctatc
     1201 gttactccac cgagggctac tataccctca atgagtgggc atcgcggcgc aacagccctg
     1261 aagacttttg ggaaacaggt aaccgacgta gtcgcgtgga gggaacgcta acgcagtcgt
     1321 tggggagaga ttatggcaat ttatacctga cattaagccg gcaacaatac tggcataccg
     1381 atgatgtcga acgattaatg caatttggct acagcagtag ctggaagcgt ctctcgtgga
     1441 acgtctcctg gagttattcc aatactgcca gacaggggac ggggaacaac catgccagtg
     1501 ataacaccag tgagcagatc tacatgctct ctttatctgt tcctttatcg ggctggtggg
     1561 gtaatagtta cgccacctat tctgtttcgc aaaacgataa ttccggtagc tcacatcaac
     1621 tcggactcag cggtacggcg ctggaaagaa ataacctttc atggaattta atgcagtcct
     1681 ataacagtca tgatgatgag gttggcggta atatgtccct gacctatgat ggctcttatg
     1741 gcacggtgaa cggcagctat aactacagcc aaaattccca gaggctgaat tatggtatca
     1801 gagggggaat tctggcacac agcgaagggg taacgttaag tcaggagtta ggtgaaacta
     1861 ttgctcttgt taaagcacct ggggccgcca ggttagaaat agataatatg cgcggtgctg
     1921 cgacggactg gcgcggctat acggtcaaga cacagctaaa cccttatgat gaaaatcggg
     1981 tagcaatcag cgataactat ttctcgaagt cgaatataga acttgataat accgtcgtta
     2041 cgatggttcc cacgcgtggt gcagtggtta aagcggagtt tgtgactcat gtgggttatc
     2101 gcgttctctt cagagtgtta aatgcaaatg gtaaaccggt accttttgga gccattgctg
     2161 cg
//
LOCUS       DAJTQQ010000233         2155 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000233 DAJTQQ010000000
VERSION     DAJTQQ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.238"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            6..224
                     /locus_tag="OO309_004202"
     CDS             6..224
                     /locus_tag="OO309_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR5849099.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="OO309_004203"
     CDS             226..591
                     /locus_tag="OO309_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5849100.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            588..1253
                     /locus_tag="OO309_004204"
     CDS             588..1253
                     /locus_tag="OO309_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849101.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..1618
                     /locus_tag="OO309_004205"
     CDS             1253..1618
                     /locus_tag="OO309_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR5849102.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2073..>2155
                     /gene="hokD"
                     /locus_tag="OO309_004206"
     CDS             2073..>2155
                     /gene="hokD"
                     /locus_tag="OO309_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5849103.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRK"
BASE COUNT          593 a          445 c          575 g          542 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaatatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga tgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata
     1441 ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt
     1501 tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt
     1561 agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga atcaatgacc
     1621 tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt ggtgtattcg
     1681 atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt ttttgaagga
     1741 aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta aacgtatctc
     1801 aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa ggtgctggca
     1861 ataaaaaata gcgttttctt atcggtatcg gtaagattgc tgcgggtgct tgaggctgtc
     1921 tgcctcgggc atgccactgt aaggcagaca gagaaaagcc ccagttaaca ttacgcgtcc
     1981 tgcaagacgc ttaacattaa tctgaggccc aatctatgtc tcacaaatgt aggttagcct
     2041 cttacgtgcc gaaaggcaag gagaagcagg ctatgaagca gcaaaaggcg atgttaatcg
     2101 ccctgatcgt catctgttta accgtcatag tgacggcact ggtaacgagg aaaga
//
LOCUS       DAJTQQ010000234         2093 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000234 DAJTQQ010000000
VERSION     DAJTQQ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.239"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(211..375)
                     /locus_tag="OO309_004207"
     CDS             complement(211..375)
                     /locus_tag="OO309_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849104.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1277..1561
                     /locus_tag="OO309_004208"
     CDS             1277..1561
                     /locus_tag="OO309_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5849105.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1549..2034
                     /locus_tag="OO309_004209"
     CDS             1549..2034
                     /locus_tag="OO309_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5849106.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          539 a          438 c          601 g          515 t
ORIGIN      
        1 tggacattat ttttgtggaa ccggaggtaa cagaccagac ggtttaaatg agccggttac
       61 tttaaacggg tgatggacag cacctcaaaa gttcagatgg attatatttg tctgcaatac
      121 cccgggtatt ttcgatttgc gaaatggatg gaacgacttg ctcaggggat agctgatgga
      181 gttactgaag taccgaagga ccactaaaaa tcacaggccg ggaatttcgg cgtttccggc
      241 gtaggtgtgc cacgaatgca tccagtggcg tgatgttgga tgcaatgctg tatgagagat
      301 gaggggcggc gtccagacgc agccttcaaa ccgccttgag ctgctgccgt taagagcggt
      361 ggtggtgagc gtcaaggtta aaaggcgcag aacatgtgtc aggtaggtgt tgcggagatg
      421 aacgaaagtg aactgtcgat gaagtgtcga aaggaacctc taggcgatgt cgaaaatgtg
      481 ctatctacgc agcgcatgac gagtccagca gatatctggt actggctggg cggcatccgg
      541 catataggca gcatgaacgc aacacaggct ctcgtacgga acgtgggaac ctgtcgtctc
      601 gatgctaagg gagcggcgcg gagcagaggc actgcaagcg cctgagtacc gatgcgagac
      661 acaggggcag atggcttcgt agtagtgagg aagtgcctgt aatgggcatg gagcgaaggg
      721 ggtcagtcgt tggggctggt ttatcggtca accggatagc cgggaggagc ccatgagtca
      781 gccaaagccg ttcgaggtct ctaagtatgc ggtttggaaa gcataccagc gggtcaaagc
      841 taatcgaggt gcggcaggtg tcgatgggca gtcagtcgag gcgtttgagg ttaccaatcg
      901 aagctatacc cggtaatggc gcaggttgac ctgcacttgg ccaagtggat tgcccgcaaa
      961 cacaagcgag cgcgaggtag tctggtgcgt gcattcgagt ggttactgcg aattcgtcac
     1021 gactgcccga cgctgtttgc acactggtgc ttagcttacg atacttgaat ggttcaacga
     1081 caagagccgt atgacgcgag agtgtcacgt acggctccgt gggagcgtag gggggcggtg
     1141 cccctgcgcc acccgatatc ccctttgagt gcggttacgt ccacagaggt aaatgccgct
     1201 gacgcggcat ctgtaatctg ttactttgtg tatatcattt gctatatatt gtcaatatgc
     1261 tattgttgta aagcaaatga tatacggagg ctttatgaaa tcaggtgttc agctcaatct
     1321 cagagcaaga gaatcacagc ggatactcat tgatgctgct gcagaaattc ttcataagtc
     1381 gcgtacagat ttcattctgg aaatggcctg taaagctgct gaagatgtta ttctcgaccg
     1441 gcgggtgttt aattttaatg acaggcaata tgaagagttc attgaaatgc ttgatgctcc
     1501 ggttgcagat gatcctgcca tcgaaaaatt gctggcaagg aaacctcaat gggatgtgta
     1561 actgcaccag aacctttatc ctcatttcat caggtcgctg aatttgtcag cagtgaagct
     1621 gtacttgatg actggttaaa gcagaaggag ctaaaaaatc aggcaatcgg agccacccgg
     1681 acttttgttg tatgcagaaa aggtacgcaa caaatagtcg gtttttactc actggccact
     1741 ggtagtgtca atcatacaga agctacaggt aaccttcggc gcaatatgcc agaccccatt
     1801 cctgtaatta tacttgcacg cctggctgta gatgtgtcat ttcgaggaaa agggcttggc
     1861 gcggatttac ttcatgatgc agttcgtcgt tgttacaggg ttgccgaaaa tatcggtgtg
     1921 cgtgcaatta tggttcatgc gcttacggaa aacgcaaaac agttttatat tcatcatggt
     1981 tttaagccat caaaaactca ggtgcaaaca ttgtttctta aactccctca atagggagta
     2041 atgccgatca gttaaggatc agttgaccga tccagtggct gtgtaagaat ccg
//
LOCUS       DAJTQQ010000235         2061 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000235 DAJTQQ010000000
VERSION     DAJTQQ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.240"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..249)
                     /locus_tag="OO309_004210"
     CDS             complement(<1..249)
                     /locus_tag="OO309_004210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849107.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEI"
     gene            complement(1002..1475)
                     /locus_tag="OO309_004211"
                     /pseudo
     CDS             complement(1002..1475)
                     /locus_tag="OO309_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(1403..1769)
                     /locus_tag="OO309_004212"
                     /pseudo
     CDS             complement(1403..1769)
                     /locus_tag="OO309_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1771..2061)
                     /locus_tag="OO309_004213"
                     /pseudo
     CDS             complement(1771..2061)
                     /locus_tag="OO309_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          471 a          578 c          483 g          529 t
ORIGIN      
        1 aatttccgga tacccttcgt catcaacacg tacaaaccag aagaccagtt ttttgtttcc
       61 ggcatccaca aagaacggaa tattcaggtc tgcgcaacac tcaacggcat cgtcaaaatt
      121 atcaaagcgc agaacctcag cttcttcttc gtcaaaaaaa tcatcttcgt gaagttttac
      181 gacatagcgg aggagtttgc ttctttgagg tgcaggtttt cgtttgcgtg gtttagctga
      241 acgggccata taactacctg aaagacaatg atattgtctg tttttataac ggtaatcaca
      301 ggccatgaca agccgcagcc gtcaggccgc ctactcgggg cggtttagaa cgcttattta
      361 gtaccagatc ttacttataa taaaggggct tggcagtttg gataaactat tgtccttatt
      421 ccactaatac aaactttttg taaagtatat taaatatact tcatttcata ctgaaatccc
      481 cggaagtatt caatcaaaca attacaccat aaaaggaaga gggggcccca attaagttgt
      541 caagcatgtt tactgcgcaa tcgcttggtg ctccgtccct gtaccccgca ttggtactct
      601 ggcgctcatg gggcttccac ttgcgcttct cccttcacat cggggtgaca ggtccacgtt
      661 ccccacaaga gcctgaatcg acttcacgcc gcttttatgc cggatgccgc gcaggcagta
      721 aacatatccc acaggaacga ctccaccatt gttttgacat catctatatg ctttcgacac
      781 atcatcagcg gttcactttc gttcgtctct tcgaaccata cctgatcggc tcacgtccga
      841 ccttttccca tctcgctcac caccacatct cttaaatgca gcagcagtgg atggtttggt
      901 gcctgcgcct gcacgccgac accaaggggc cttccctcat ctcttgtgct gcatggctgc
      961 acagggactc acgtccccag gacgcgcctt cgtggcacac agtcatcggc atagcaacag
     1021 taagcaacgg caggtttcca ttgccagttt tctttgacag cagtacttcg gcctcgctgg
     1081 atactgtgat tccagtacca gcgatccttt ctggcttttc cactcaggta gcgtttattc
     1141 agatactggt cgaactcatt cagtatgatg ttcgacagca acggcgagat cacaccgccc
     1201 tgtggcatac tttcactgac cgcccggaag agaccaacat caacacggcg ttttccacag
     1261 cagtctcata aaacgtgtat cactgatcct gcggcagacg gctttcatca gcagccggtg
     1321 atgtactgtg tcgaagtagc tggacaggtc accttcaata acccggcgcc ccgtgtttcc
     1381 ccgcagtcgg tgagctgtaa tagtacgtga tgtctcccgc ccacttctgg ttcgggccac
     1441 tggcgtaaaa atcctgcgtc agcagatttt ctgatacagg caggccgtgt gcacggtaac
     1501 tgatcgggct gaacttctgg gaggcttttg cgctcagttc ctgacggcgc agactggctg
     1561 ccacggtttt tacgttaaag tggtaaccag agcacacagt tcatccgtca ggcgtggggc
     1621 accgtaacgc tggactgggt aaaagccttg cggacaacgc tgtcgcagtg ctgacggaac
     1681 tgcggacgtg tgtttatcct tgtccgccgc tgacaccacg tataccagcc gctgcgggcc
     1741 acccggagta cgcggcacat tgccttgatg acggtttacg cggtcgcctg cggcaacggc
     1801 tttccggctg acggtagtgt ggaccttttt accccggaga acaccggcaa gccccataac
     1861 cgccatgaga cgtgccacag tgcatctggc cactctgata ccttcccgta acaactgatg
     1921 ccagacttta cgcacagcgt atacctgatg attctcatcg tatacgcgca gtatctcttt
     1981 cttcagccag tcatcgcgct gcgcacgggc actgcgttta tcatgatgat gtcgctgttg
     2041 ctgacagtgg taatacgttg a
//
LOCUS       DAJTQQ010000236         2051 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000236 DAJTQQ010000000
VERSION     DAJTQQ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.241"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            17..1366
                     /gene="ccp"
                     /locus_tag="OO309_004214"
     CDS             17..1366
                     /gene="ccp"
                     /locus_tag="OO309_004214"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005031735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR5849108.1"
                     /translation="MCYLGLSGYVWYHDNKRSKQADVQASAVSENNKVLGFLREKGCD
                     YCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAALLADKPVSQSDLNKIEW
                     VMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYASNDTAPEHRNEPVQPI
                     PQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGVDGRKTSIGVGGAVGPI
                     NAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSWDEIIAKLEKDPQLKAQ
                     FLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENALTAQQKKGYQLFKDNK
                     CATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKEERDKLRQKVPGLRNVA
                     LTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFLHSLNGVYTPYMQDKQ"
     gene            1577..>2051
                     /locus_tag="OO309_004215"
     CDS             1577..>2051
                     /locus_tag="OO309_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5849109.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLV"
BASE COUNT          557 a          429 c          529 g          536 t
ORIGIN      
        1 cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt accacgataa
       61 taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata ataaggtttt
      121 aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag aattacccgc
      181 ctattattat attcctggcg cgaaacagtt gatggattac gacattaagc ttggatataa
      241 atcttttaac ctcgaggccg tgcgtgcggc actgctggct gataaacccg tttcgcaaag
      301 cgatctgaat aagattgaat gggtgatgca gtatgaaact atgccaccaa cgcgttatac
      361 cgcgctacac tgggcgggta aggtgagtga tgaagagcgg gcggaaatac tggcctggat
      421 tgcaaaacag cgcgcggaat attacgccag caatgatact gctccggagc atcgcaatga
      481 accggtgcag cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg cgttgggctt
      541 tgcgctgtat cacgatcccc gtttatcggc tgatagcacc atttcatgcg ctcattgcca
      601 tacgttgaat gcggggggcg tcgatggcag aaaaacatcg attggtgttg gtggcgcagt
      661 tgggccgatt aatgcgccga cggtatttaa ctcagtattt aacgttgagc agttctggga
      721 tggtcgtgcg gcaacattgc aggatcaggc aggtggaccg ccgttgaacc cgattgaaat
      781 ggcgtcgaaa tcctgggacg aaattattgc taagctggaa aaagatccgc agcttaaagc
      841 gcagttcctc gaagtttatc cgcaaggttt cagtggcgaa aatattactg atgccattgc
      901 tgaatttgag aaaacattaa ttacgccgga ttccccattt gataaatggt tgcgtggaga
      961 tgaaaatgcg ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag ataataaatg
     1021 tgcaacttgt catggtggta ttattctcgg cggacgttcc tttgaaccgt tggggctgaa
     1081 aaaagacttt aactttgggg aaattacggc ggcggatatt ggtcgtatga atgtgactaa
     1141 agaagagcgt gataaattgc gtcagaaagt acccggttta cgtaacgttg ctttaacggc
     1201 accgtacttc catcgcggtg acgtgccgac gctggacggg gcggtgaaac tgatgctgcg
     1261 ctatcaggta ggcaaagagc tgccgcagga ggatgtggat gatatcgtag ctttcctgca
     1321 cagtctgaac ggggtgtaca cgccgtatat gcaggataaa caataattaa tttgatcgcc
     1381 cgaacaggaa tgtttgggcg atttttatta cgataataaa gtctgttttt aatattatca
     1441 tgttaaatgt ttatattata aaaagtcgtt tttctgctta ggattttgtt atttaaatta
     1501 agcctgtaat gccttgcttc cattgcggat aaatcctact tttttattgc cttcaaataa
     1561 atttaaggag ttcgaaatgg accagaagct gttaacggat ttccgctcag aactactcga
     1621 ttcacgtttt ggcgcaaagg ccatttctac tatcgcggag tcaaaacgat ttccgctgca
     1681 cgaaatgcgc gatgatgtcg cattccagat tatcaatgat gaattatatc ttgatggcaa
     1741 cgctcgtcag aacctagcca ctttctgcca gacctgggac gacgaaaacg tccataaatt
     1801 gatggatttg tcgatcaata aaaactggat cgacaaagaa gaatatccgc aatccgcagc
     1861 catcgacctg cgttgcgtaa atatggttgc cgatctgtgg catgcgcctg cgccgaaaaa
     1921 tggtcaggcc gttggcacca acaccattgg ttcttccgag gcctgtatgc tcggcgggat
     1981 ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca ggcaaaccaa cggataaacc
     2041 aaacctagtg t
//
LOCUS       DAJTQQ010000237         1911 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000237 DAJTQQ010000000
VERSION     DAJTQQ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.243"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(30..1181)
                     /locus_tag="OO309_004216"
                     /pseudo
     CDS             complement(30..1181)
                     /locus_tag="OO309_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1246..1894
                     /locus_tag="OO309_004217"
                     /pseudo
     CDS             1246..1894
                     /locus_tag="OO309_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          475 a          480 c          421 g          535 t
ORIGIN      
        1 gatcctaccc acgtaatgtg gacacggccc taagcgagat tatggttttc aaattgttcc
       61 gggctgagac cgccacaggc actgtgacga cgccagcgat tgtaatcaca ctcgatataa
      121 ttaaacaccg ttgcccgcat tatttcccgg ctgctaaagc gttccccgtg gatacattcc
      181 accttcagcg aatgaaagaa gctttccaca caggcattgt cataacagta gcctttcgca
      241 ctcatactgc cacgcaggtt gtgtcgcttc agcattgcct gataatcccc tgaacagtat
      301 tgaccaccac ggtccgtatg aacaatgacg ctttccgggc gtcttctccg ccacaacgcc
      361 atttgcagtg catcacaggc cagttgtgct gtcattcgcg gtgacatcga ccagccaatg
      421 acggcgcgtg accacaggtc gatgactact gcgagataca accagccctc atcggtacgc
      481 aagtacgtga tgtcacccgc ccacttctgg tttcgggcca ctggcgctga agtcctgctc
      541 cagcagattc tccaatacgg gcaggccatg tgcacggtag ctgaccgggc tgaacttccg
      601 gctggctttc gcccgcagcc cctgacgacg caggctggcg gcaatggttt taatattgaa
      661 ctccggcagt tcgtcagcaa ggcggggagc accgtatcgc tgctttgcct caaagaatgc
      721 cttatggaca gcggcatcgc aggtgagccg aaactgttgg cgcaggctca tctggtgacg
      781 acgcctgagc cagacatacc agccgctgcg ggcaacccga agtacacgac acatcgcttt
      841 gatgctgaac tctgcccgat gattttcgat aaagacatac ttcatttcag gcgcttcgcg
      901 aagtatgtcg cggccttttg gaggatggac agttcctcag cctgctccgc cagttgtcgt
      961 ttaaggcgga cattttcagc ggccagttcg ctttcgcgct ctgacgaact catttgttgc
     1021 tgctgtttac tgcgccaggc ataaagctga gattcataca ggctgagttc acgggctgcg
     1081 gcggccacac cgatgcgttc agcgagtttc agggcttcgt tacgaaattc aggcgtatgt
     1141 tgtttacggg gcttcttgct gattgatact ggttttgtca tgagtcacct ctggttgaga
     1201 gtttactcac ttagtcctgt gtccactatt ggtgggtaag atcaccctcc attaaaacta
     1261 aaacgggcgc tttctgatat atcatctgta agcgcagtaa taccattagt agcacctcgc
     1321 gcattgatta ttgaatatta taactggaat gttatatttc ttgttatgat gctgttcagt
     1381 attataataa tgttattatc accaaagata actcatgcaa atgagaaagt aatatgtcaa
     1441 aacagtgcat cctttgtgac caaagagttt atctgcggca ctatacttgt ctcattaata
     1501 ctcagttctc tattttgcta catatcaata tcaccatcaa ttttcatgca tacttatggc
     1561 ctgagtactt ttagttactc aattattttc tccttctcag ttttattttt cataatcgga
     1621 aatcaattat caaagattga aaaattatcc aacctatggt ttttattacc caatagccaa
     1681 ttttatcgca ttacaaataa atggttcaaa aacaggattt gccgcatcta ttacaggttt
     1741 tactcaaaca ataagcgctg gtacaatcag tttcctgtca gtaaccgtaa gcgacaacgg
     1801 tgttggtccc ggaactactt taggtcttgg ttcactaata acttcattac tgtcaatagc
     1861 cgtaatcgcc attgcaggaa ataataaaaa gtaagtgtac tctgactgcg t
//
LOCUS       DAJTQQ010000238         1903 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000238 DAJTQQ010000000
VERSION     DAJTQQ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.244"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(114..1568)
                     /gene="ospC2"
                     /locus_tag="OO309_004218"
     CDS             complement(114..1568)
                     /gene="ospC2"
                     /locus_tag="OO309_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR5849110.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFISNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          608 a          325 c          293 g          677 t
ORIGIN      
        1 tcaccacatg caatgtgttg ttgcagggga acggtctgca ccatccccta tatcgatcaa
       61 tataacaaaa atgacaacgt cagattcagg gcacaaccta aatatatttt aggtcacctc
      121 ccaaatcgtt tgcctgatac cgctccattt tttaataaaa aatcaattgt ttttgaatct
      181 ttgcttttca ttgcattgtc aagcatagtg tccccactat ttgctttttg aaatcttttg
      241 tttacatcaa ccaatccatt actaataaaa tattctagca ctttatagtt ggcactatct
      301 tcactaagta aatactcaac atcatacagc tcacaataca tattgttgtt cattttctcc
      361 atctctgcga tatcttgttt tgtaaaatta aatttagata gtaaataaag tgccatatca
      421 gattttgcat tgcctagaga ataagcaaga gcttgatgag ccattttttt atcagtcacc
      481 ttattgttaa tttcttcata attggatgct ttaatggcat cctccaaaga tatatctctg
      541 agtcttatct ttttgaagtt atctgtgctt agcattcttg gtatatgata ctcaagctga
      601 aacacaaagt ttataattct atccagacta acaggatcaa tgtttttatt ataacaaaac
      661 tctcggaatc tttgatcttt actctttctt ataaaatcaa taagatgcaa cccaattcca
      721 agcctcatat ctttagtatt aaatattggc acatcagttt ttccctgatt accatgcaca
      781 tacctgctaa cttcatccac aacctcttta aagttatcta agaaaaatga ttggtgctca
      841 tggtaaaaaa caggtggaat agcattatca aagtggtcgg ttaatgtcat atatccatat
      901 ggagagtcat gatcaatgat atacgcatta gcaccaaaat ccacagtatg atgtgtttta
      961 tttagtggga gtgtttcttg atgattggaa atttcaaccc caaaaaaaac aaaatcgtgg
     1021 ttagctattt cttcaatatc ctcagagaat gtgtttttat atgtttgtat attctcagac
     1081 ttaattttat caaaggactt aatattaaga gtattatttg acaggttagc gttactttga
     1141 tgtctgaagt taaaatcaga attaataata atagagagca agtgacgttc atccgaagaa
     1201 agaggatgag ttttttgtat tatttcagaa agatagtgtc tggacacctt atcgaggtgc
     1261 tcaagtgacc gaaatgatga ccgtgctcca gatggcgctt caatagaaag atttaatgat
     1321 ttgaaactgg tcccttgaga gaacatttta ctataacttt gcgctgctat agacttcctt
     1381 aaaaagcttt tcacagtatc agcacagtga tctaaatgtt tttcacttat gccagaagag
     1441 gatgagtttg ttacgcatga tatgtttttt tgtaatgctt ttgtatcttt gttcggattt
     1501 atttttccat caacaagatc aatattgttc tgaacattaa tatgatttac tgcttcaggt
     1561 attttcataa aacatccccc attatgtgcg tttttatctg cagttattta gaataatatt
     1621 atctaactta atattttctt gtatgtatta taacgaacat attttaatat gttgtacgat
     1681 tttcactctg aaatcccctg agtttaatca cacttccttt actgaaagga attattgcaa
     1741 cagtattatt tgtttttgga tatatatctt gcgccttgtt atgtagttaa tgctcagaat
     1801 atgatagata aaatagagac gcaaaacagg gcatgctcat aacatgatga taacataaaa
     1861 aattagtaac aataaatgaa agaatcatgg gcgctcaaca aaa
//
LOCUS       DAJTQQ010000239         1874 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000239 DAJTQQ010000000
VERSION     DAJTQQ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.245"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            1..68
                     /locus_tag="OO309_004219"
                     /pseudo
     CDS             1..68
                     /locus_tag="OO309_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA4"
     gene            54..358
                     /locus_tag="OO309_004220"
                     /pseudo
     CDS             54..358
                     /locus_tag="OO309_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            527..>1874
                     /locus_tag="OO309_004221"
     CDS             527..>1874
                     /locus_tag="OO309_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5849111.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AASGLSGTFSYIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFTLMSRERSL"
BASE COUNT          477 a          364 c          433 g          600 t
ORIGIN      
        1 agtcggggcg tatctccgcg ttagcgggcc gtgagggccg cttacgagcg tgtatgagag
       61 acgtacagtg gttttgtccg ctcacaccag tacatacagg tacggaaata aaaccagtcg
      121 aaatgccggt aacgacgatt actcctgtct ctgatgccgg tggactacaa gattttattt
      181 actggcgtcc tgatgcggac agggctggta tggaacctgt ttatgtgata cttaatgatc
      241 ctctggattc cgggcgattt acgcgtaaac aactaaataa aaatatctta aacatgcaag
      301 taattttggt attctcgata caagaataaa tagtagtaac tactatagct gcaaataatg
      361 ttaaaaacat ttctatgatc atctttacat ttttcatgat tgacaattgt ttttggttaa
      421 aaaaagacaa ctaatgagtg tgataatgca atactttgag tgattatata atgataagta
      481 aacaggtttg cgacaattgg ttatcagtat tacagaagga ttaaatatgt cattattcac
      541 agaacctaag gaaattgaaa gattaccctc tgaagagatt gaacgattat atcccgtcct
      601 gcgatacagg gtctttatca gcattttcct cggatatatg gggtattatt ttgtccgaaa
      661 caccaccagt gtgttatccg gtgtgctgca catgtctgca accgagatcg gcattatctc
      721 ctgtgccggt tttttatcct atggtatatc aaagtttgtt tccggactta tttctgacag
      781 aagtaattca aaagtatttt tgtctcttgg tttattcctg tccggactgg taaattttct
      841 gataggatat attcccggga tcatcacatc cgttacgtta ttttcaacaa tgtatttact
      901 taacggctgg atccaaggta tgggatatcc tcctggtgcc aaaacactgg tgttctggta
      961 tgaacatcgg gaaaggatca cctgggccac actgtggaat ttatcgcaca atgtcggggg
     1021 agctctggcc cccgttctga ttggtttcag ttttggtttt ttcggcgaca gtgctctgga
     1081 tcatgcccgg gccgcattta tcttcccagg agtactgtgt atggcaatga gtgtgctgat
     1141 ttactttatt caggtggaca gacctgtatc agtggggctt cctcccattg aagaatggaa
     1201 aggtaacgtc gtttcacacc cggctaaagg ccgtgaacaa ggtccgagac tctccattcc
     1261 tgatatcatc agaaaacata tcatccgaaa taataaactc atttactgct gtatctatgg
     1321 ttcgtttgtt tatattctga gatacggtat tgtttcctgg gctcctaagt ttttaagtga
     1381 cagtctggat gtcggtggta aggatatggg aaaactggca tcaatggggg ggggttctgt
     1441 ttttgaaatc ggtggtgtgg ctggtatgct tcttgccgga tacctgtctg tcagactgtt
     1501 cagaaactca aaaccattaa caaatacttt atttctggca cttaccatta ttttattaat
     1561 cgcgtactgg tacgtgccat caggcaatga atatctctgg ctaaactata caatccttat
     1621 tctgttgggg ctggcagtgt atggtccggt gatgtttatt ggtctttatt caatggagct
     1681 tgttcctaaa gaagctgccg gagccgcatc agggctgtca ggcacatttt cctatatctt
     1741 cggctcaatt gttgcgacac tgggaatggg acttgttgtc gattatctgg gatggggggc
     1801 cacatttatc gtactgatac tgtctgctgt ttttgccatc attttcacac tgatgagtcg
     1861 tgaacgctca ctgg
//
LOCUS       DAJTQQ010000240         1837 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000240 DAJTQQ010000000
VERSION     DAJTQQ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.246"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..417)
                     /gene="arsC"
                     /locus_tag="OO309_004222"
     CDS             complement(<1..417)
                     /gene="arsC"
                     /locus_tag="OO309_004222"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR5849112.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKR"
     gene            complement(430..1719)
                     /gene="arsB"
                     /locus_tag="OO309_004223"
     CDS             complement(430..1719)
                     /gene="arsB"
                     /locus_tag="OO309_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR5849113.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1773..>1837)
                     /locus_tag="OO309_004224"
     CDS             complement(1773..>1837)
                     /locus_tag="OO309_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="regulates the expression of of the arsRBC involved
                     in resistance to arsenic; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5849114.1"
                     /translation="AIVRNLARQNCSGDSKNICS"
BASE COUNT          492 a          515 c          461 g          369 t
ORIGIN      
        1 gcgcttaccc gcttcatcaa ccactttctc gccatcttcc ttggagaatg cgcctttttg
       61 cgcatctggc agaatttcca gcaccacttc tgaagggcgg cacaggcgag ttcccagcgg
      121 cgtcaccaca atcgggcgat taatcagaat cgggtgctga agcataaagt cgattaaccg
      181 atcgtcagta aatttatctt ccgcaaggcc cagctcctca tacggttcga cgtttttacg
      241 cagcagcgcg cgtacggaaa tccccatatc ggcaatgagt ttgaccagtt catcgcgcgt
      301 tggcggagtt tccagataat ggataatggt cggttctgtg ccgctgttgc ggatcatctc
      361 cagcgtatta cgcgacgtgc cgcaggccgg gttgtgataa atggtaatgt tgctcatatc
      421 agtatctcat tacaaagtga aagagagacg tagcgccagc gcagccagcg tcacaaacag
      481 cacaggcagg gtcatgataa tccctgtacg gaagtaatat ccccagctga tagtcatatt
      541 cttctgcgaa agtacgtgca gccagagcag cgttgccagg ctaccaatcg gggtaatttt
      601 cggccccaaa tcgcagccaa tcacgttggc ataaaccatc gcttctttga taacgccaga
      661 tgccgtgctg ccatcaatgg acaacgcgcc aaccagtacc gtcggcatat tgttcataat
      721 agaagagagg aaggcggtga ggaatccggt gccgagcgtc gcggcccaca ggccgttatc
      781 cgccagcacg ttgagtacgc cagaaagata ttccgttaat ccggcattgc gcaggccata
      841 aaccaccaga tacatgccga gcgagaagat gacaatctgc cagggggcac cgcgcaggac
      901 tttacccgta ttaatcgcat gaccgcgttt agcgacgaca aataatatca gtgcgcccac
      961 agctgcaatg gcgctcaccg gaatgccgag cggttccagg acgaaaaatc ccaccagcag
     1021 aagcagtaaa acaacccagc cagttttgaa cgtagcagga tctttgatcg cttctgcggg
     1081 agatttcagc agcgccatat cgtagttctg cggaatatct ttgcgaaaat agagatgtag
     1141 catcaccagc gtggcaacaa tcgcggcgat atccaccggc accatcaccg aggcgtattc
     1201 gcgaaagccg aggccaaaga aatcagcgga aacgatattc accaggttgg agacaataag
     1261 cggcaggctg gcggtatcgg caatgaatcc ggccgccatc acgaacgcca gcgtcgtgcc
     1321 tttactgaat cctaaagcca gcagcatggc gatgacaatc ggcgtcaaaa taagcgccgc
     1381 gccatcattg gcaaacaggg ccgcaacggc ggcaccgagc aggacaatcc aggtaaacag
     1441 caagcgaccg cgtccattgc cccagcgtga aacgtgcagc gctgcccatt caaaaaagcc
     1501 ggactcatcc agcagcaggc tgatgataat gacggcaata aacgccgccg tcgcgttcca
     1561 gacgatattc cacaccaccg gaatatcacc cggatgaacc acgcccgtaa ccaacgccag
     1621 taccgcgccg agcgttgcac tccagccgat gcctaaacct ttcggctgcc agataaccaa
     1681 tacgatggtc aggacaaaga tagcgcctgc cagtaacata atgcctcccg gataaaacac
     1741 atgtgcaaat ccatatgtgt ttagctaaat ttttaactgc aaatgttctt actgtctccg
     1801 gaacagtttt gtctggccag gttgcggaca attgcct
//
LOCUS       DAJTQQ010000241         1816 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000241 DAJTQQ010000000
VERSION     DAJTQQ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.247"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(198..599)
                     /locus_tag="OO309_004225"
                     /pseudo
     CDS             complement(198..599)
                     /locus_tag="OO309_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1028..1816
                     /gene="virF"
                     /locus_tag="OO309_004226"
     CDS             1028..1816
                     /gene="virF"
                     /locus_tag="OO309_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR5849115.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          565 a          329 c          337 g          585 t
ORIGIN      
        1 tagtttgtgt aagcatattg cttccttatg ttatatttta gtaaagaatt tattttttac
       61 attatagtcg gctaaatgaa gtttactgta aaaaaacgat acttttttcg taagcgatga
      121 gatgtggagc cagaaaaatg gctgcgttac gtcattgaac tgtccttgac ccacgatatt
      181 cagacagtcg ttgtatctta agttgacatt acccgctgac gtcgatattc ccttggtgag
      241 agataaccca acgcactatg cggatggttt tcattgtaat gctcgatcgc cactgcaagg
      301 ttatgcaatg ccgttcttgg tttcggcatg aacgcgatgc agtcttcctt catcgttttc
      361 atgaaccgtt ctgctattcc attactctga gggctactga ctgccgtcgt gcagggctcc
      421 agattcaact ctctggcgaa ctgccgtgtt tcatgagccg tataagccga accgttatcg
      481 gccactggat tggccgttcc ggtcctttat cgccatatcg ctcctgacat gacatcctgc
      541 accgttgctt tgtcataatc tcccgtgcct gctgcccagt ctatggcttc acgatcgcaa
      601 tatggaacgt aacgctgtca tcgggagaat cgtcaatgcg gcattcggtt aacgcttacc
      661 aatatcaaca ccaaccggat ttgtggagcc tccagtctga aggggcttta tgcgttccgt
      721 ataggatatt atgatgctgg agttttgcga agctccactc ctttccgaac atttacacag
      781 atattgccta agaaaagtga aaccataata tggttatagt ccctttcagt gcaaatactt
      841 agcttgttgc acagagaaat agaagctgca taagctcttt cttcaaaaaa tgtaaataaa
      901 gttaaatata ggaaaaatta cttaatctat cttaataacg gaaagatttt gtatacaatc
      961 actgttacag aaatttctta gttactctgt aaacactaaa tatagtttgg tatattctgt
     1021 tgaatttatg atggatatgg gacataaaaa caaaatagat ataaaggttc gcttgcataa
     1081 ctatattatt ttatatgcaa aaaggtgttc aatgacggtt agctcaggca atgaaacttt
     1141 gactatcgat gaagggcaaa ttgcttttat agagcgaaat atacaaataa acgtctccat
     1201 aaaaaaatct gatagcatta atccatttga gattataagc cttgacagaa atttattatt
     1261 aagcattatt agaataatgg aaccaattta ttcatttcaa cactcctatt ctgaggagaa
     1321 aagggggtta aacaaaaaaa tattcctcct ctctgaggag gaggtttcta tcgatttgtt
     1381 caaatctata aaagagatgc ctttcggcaa aagaaagatc tatagtttag cttgcctttt
     1441 atcagctgtt tctgatgagg aagctttata tacttcgata tcgatagctt cttctcttag
     1501 tttttctgat cagataagga agattgttga aaaaaacatc gagaagagat ggcgtctttc
     1561 tgatatttca aataacttga atttatcaga aatagctgtt agaaaacgat tggagagtga
     1621 aaaattaaca tttcaacaaa tccttcttga tattcgcatg catcatgcag caaagctttt
     1681 attgaatagt caaagctata ttaatgatgt atcaagactt atcggaatat caagcccatc
     1741 ttattttata aggaaattta atgaatatta tggtataact ccaaagaaat tttacttata
     1801 tcataaaaaa ttttaa
//
LOCUS       DAJTQQ010000242         1771 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000242 DAJTQQ010000000
VERSION     DAJTQQ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.248"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(168..1382)
                     /locus_tag="OO309_004227"
     CDS             complement(168..1382)
                     /locus_tag="OO309_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR5849116.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          536 a          431 c          362 g          442 t
ORIGIN      
        1 acgatccccg ccataaataa taaaaccagg gtcactgtct gaatacgacg aatccgttgc
       61 ggtttattac ccgcaacact ggttgacaca tcaacgtcca tttttagctc cttgagcgga
      121 attaatcgat ttgatgacta ttcagaaaat aattaagcga accgttatta ccagttcaac
      181 aatgtgccat cttccagtcg tgcgacgggg agatactccg ggtcatacgg atatttcgcc
      241 gccagtttct catcgaaaga aatacccagc cccggttcat cgcctgggtg catataacct
      301 tcctcgaagc gccagctgta cgggaaaact tcaagcattt gttcggaata gcccatgtac
      361 tcctgaacgc caaaatttgg tacccatagg tcaaaatgca gcgccgccgc atgacaaaca
      421 ggcgataaat ctgaagggcc atgcgaaccg gtacgtacct gataaagcga tgcaaaatcg
      481 gcaatccgcc gcatcccggt aatgccgcct gcatgggtga tcgtcgtgcg aatatagcca
      541 atcagctgct cttcgattaa ctgcttgcag tcccagatac tgttaaacac ttcaccgacc
      601 gcaatcggcg tcacggtatg ctggcgaatc agacgaaaac attcctggtt ctcggcaggc
      661 gtcggatctt ccatccagaa caggcggtaa tcctcaatgc tcttcccaaa gcgcgccgct
      721 tcgatggggg tcaggcgatg gtgcatgtca tgcaacagat gttcgttaaa accgaactta
      781 ttacgcaccg cttcgaataa cttcggcgta aaatcgagat acttttctga agaccacaac
      841 tgctcttctg gccacaggcc tttggttgcc ggttcatacg ccagtccttt gcccttcgcc
      901 aggccataag tggtttgcat acccggtaca ccgcactgca cacgaatcgc cttaaagccc
      961 atctgctggt gctttgcata gtcttcaagg acttcatcaa tcgtacgccc ggtggtgtgg
     1021 caataaacca tcacgccttc acgagaagca ccaccaagca gttgatacaa tggcatattt
     1081 gccgctttag ctttgatatc ccacaacgcc atatcaatgg cagaaatggc tgacatcgtg
     1141 acggggccac gtcgccagta agcaccttta taaaagaatt gccagatatc ttcgatacgg
     1201 ctggcatcgc ggccaattag ttgtggacaa agatgatctt tcagataaga agcgaccgac
     1261 aattcgcgac catttaacgt ggcatctcct aatccacaaa gtccgctttc agtggttatt
     1321 ttaagcgtaa cgaaattacg ccctgggcaa gtaacaaaaa catctgcagc aataattttc
     1381 attattgcaa tctcctgtgt tgtaaataga cgtttcaaac ctaattttac gcctactacc
     1441 atacaagaga gaaaaagcga ttattcaacc gtgatcacat taaaaacaaa aatttctacc
     1501 gaggagaaaa aataccctca aaaaccacct gaataattaa ccattattgt taaaacaaca
     1561 cgttgaataa tcacataaca aactccatta atcattgaaa atgagcacag aaatagaaat
     1621 taattatatt caattaatta aaatgtgtat tgcttgatgt atttaaaaat attattttca
     1681 tggtgagagt ggaggtaaag aagacacaat aataaaaagg aatgtgacaa ataatggttc
     1741 tggttgggcg ttaaatttaa ggtcaccaga a
//
LOCUS       DAJTQQ010000243         1764 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000243 DAJTQQ010000000
VERSION     DAJTQQ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.249"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            154..1179
                     /locus_tag="OO309_004228"
     CDS             154..1179
                     /locus_tag="OO309_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5849117.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1247..1764
                     /locus_tag="OO309_004229"
                     /pseudo
     CDS             1247..1764
                     /locus_tag="OO309_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          414 a          412 c          446 g          492 t
ORIGIN      
        1 ttttattatt tcctgtctac actcagctta ttgcgacgcg aaatcgtgcg caaaagctgg
       61 cactactttt gcttataaga ggatggccac agacaggtaa aagacgtttc ccaaacgtcc
      121 tataacgata attttcgcct cacaggaagc attatgaaaa agatgatgat agccacactg
      181 gctgccgcca gcgtgctgct tgccgttgca aatcaggcgc atgctggcgc gacgcttgat
      241 gccgttcaga aaaaaggttt tgtgcaatgc gggatcagtg atggattacc taggttctct
      301 tatgccgatg cagacggtaa gttttcaggt attgatgttg atgtttgtcg tggtgttgcc
      361 gctgctgtat ttggtgacga cacgaaagtg aaatataccc cactcacagc aaaagaacgc
      421 ttcaccgctt tacagtcagg ggaagtggat ttgctctccc gtaatacgac ctggacttca
      481 tctcgcgatg ccgggatggg aatggcattt accggcgtca cttattacga cggcattggc
      541 ttcctgacgc acgataaagc ggggctaaaa agcgcgaaag aactggatgg cgctaccgtc
      601 tgtattcagg cgggtactga taccgaactc aacgtcgccg actatttcaa ggcaaacaat
      661 atgaagtaca caccagtgac tttcgatcgc tctgacgaat cagcgaaggc actggaatct
      721 ggtcgctgcg atacgctggc ttcggatcag tcgcaactgt atgccttgcg catcaaatta
      781 agcaacccgg ctgaatggat tgtcttaccg gaggttatct ctaaagaacc gcttgggccg
      841 gtagttcgtc gtggcgatga tgaatggttc tcgattgtac gctggacgct tttcgccatg
      901 ctaaatgctg aagagatggg catcaattcc cagaacgtcg atgaaaaagc ggctaatcca
      961 gcaacgcctg atatggcaca tctgttgggt aaagaaggcg attacggcaa ggatctgaag
     1021 ttggataata aatgggccta taacatcatc aaacaggtga gtaactactc ggaaattttt
     1081 gagcgtaacg taggttcaga aagcccgctg aaaattaaac gtgggcaaaa taatctctgg
     1141 aataacggcg gtattcagta cgcgccgccc gtgcgttaag tcggtgtgag gtaacgagcg
     1201 tcgctacggc ggcgctctct tcagcgttat gctttccgag gtttctatgc ctcatcgccg
     1261 ctcaaccgtt aaaggctcac tctcctttgc caaccctacg gttcgtgcct ggttattcca
     1321 gatccttgcc gttgttgctg ttgtcggcat tgttggttgg ttatttcaaa acactgtaac
     1381 gaatctcaat aatcgtggca ttacttcagg ttttgccttt ctggatcgcg gcgctggctt
     1441 cggtattgtc cagcatttga tcgattacca gcagggcgac acctacggac gcgtttttat
     1501 tgtcggctta ctcaatacgc tactggtttc tgcattgtgt attgtgttcg cttctgtttt
     1561 gggcttcttt atcggtctgg cgagactttc ggataactgg ctgctacgaa agctttccac
     1621 aatttatatt gagatcttcc gtaatattgc aaatcttctt ctggtacttt gccgtgttgc
     1681 gcaatttgcc cggaccacgc caagctgtga gcgcgtttga tctggccttt ttgagcaatc
     1741 gtgggcttta tattccgtca ccgc
//
LOCUS       DAJTQQ010000244         1724 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000244 DAJTQQ010000000
VERSION     DAJTQQ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.250"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            1..1724
                     /gene="sat"
                     /locus_tag="OO309_004230"
                     /pseudo
     CDS             1..1724
                     /gene="sat"
                     /locus_tag="OO309_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
BASE COUNT          653 a          284 c          333 g          454 t
ORIGIN      
        1 tacggcatga tgatttataa aaatgataaa acatttcgta atcttgaaat attcggagac
       61 agcggctctg gagtatactt atatgataac aaactagaaa aatgggtatt agtcggaaca
      121 acccatggta ttgccagcgt taatggtgac caactgacat ggataacaaa atacaatgat
      181 aaactggtta gtgagttaaa agatacctat agtcataaaa taaatctgaa tggcaataat
      241 gtaaccatta aaaacacaga tataacatta caccaaaaca atgcagatac cactggtact
      301 caagaaaaaa taactaaaga caaagatatt gtgttcacaa atgggggaaa tgttctgttt
      361 aaggataatt tggattttgg ttgcggtggt attatctttg acgaaggcca tgaatataac
      421 ataaacggtc agagatttac atttaaagga gcaggaattg atatcggaaa agaaagcatt
      481 gtaaactgga atgcattgta ttccagtgat gatgttttac acaaaatagg ccccggtact
      541 ctgaatgttc aaaaaaaaca gggggcaaat ataaagatag gtgaaggaaa tgttattctt
      601 aatgaagaag gaacatttaa caatatatac cttgcaagcg gaaatggtaa ggtaatacta
      661 aataaagata attcccttgg caatgatcaa tatgcgggga tattttttac taaacgtggt
      721 ggcacgctag atttaaatgg acacaatcag acttttacta gaattgccgc cactgacgat
      781 ggaacaacaa taactaactc agatacaaag aaagaagccg ttctggcaat caataacgaa
      841 gactcctaca tatatcatgg gaacataaat ggcaatataa aactaacgca caatattaat
      901 tctcaggata agaaaactaa tgcaaaatta attctggatg gtagtgtcaa cacaaaaaat
      961 gatgttgaag tcagtaatgc cagtcttacc atgtaaggcc atgcaacaga gcatgcaata
     1021 ttcagaagca cagcgagtca ttgctccctg gtatttcttt gtggaacgga ctgggtcacc
     1081 gttttgaaag aaacagagag ttcatataat aaaaagttca attctgatta caaaagtaat
     1141 aatcagcaga cctcatttga tcagcctgac tggaaaaccg gggtgtttaa atttgataca
     1201 ttacacctga acaatgctga cttttcaata tcacgcaatg ccaatgttga aggaaatata
     1261 tcagcaaata aatcagctat cacaatcggc gataaaaatg cttacattga taatcttgca
     1321 gggaaaaata ttactaataa tggttttgac ttcaaacaaa ctatcagtac taatctatcc
     1381 ataggagaaa ctaaatttac aggtggcatc actgcacata acagccaaat agccataggt
     1441 gatcaagctg tagttacact taatggtgca acctttctga ataatactcc tataagtata
     1501 gataaaggag caaaagttat agcacaaaat tccatgttca caacaaaagg tattgatatc
     1561 tccggtgaac tgactatgat gggaatccct gaacagaata gtaaaactgt aacgccgggt
     1621 ctccactacg ctgctgatgg attcaggctg agtggtggaa atgcaaattt cattgccaga
     1681 aatatggcat ctgtcaccgg aaatatttat gctgatgatg cagc
//
LOCUS       DAJTQQ010000245         1696 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000245 DAJTQQ010000000
VERSION     DAJTQQ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.251"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(141..962)
                     /locus_tag="OO309_004231"
     CDS             complement(141..962)
                     /locus_tag="OO309_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5849118.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(977..1333)
                     /locus_tag="OO309_004232"
     CDS             complement(977..1333)
                     /locus_tag="OO309_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5849119.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(1346..>1696)
                     /locus_tag="OO309_004233"
     CDS             complement(1346..>1696)
                     /locus_tag="OO309_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5849120.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT          418 a          490 c          349 g          439 t
ORIGIN      
        1 gcagaataat aacggtccgt aattaatttc gcaataaaaa aacccgctca gcggcgggtt
       61 gtagaaactc ttctaacgcc aggcataaaa cgcccatcat tatgacgaat ttaccacaga
      121 ttccggaaaa atcaaccttg ttacctagtt acctttttta actgccgctc agcccatgct
      181 tcttcaatat caaaccgggt caccagcgca tcataaaatt tcttaactgt tttttcccat
      241 gacgcgcgtg ttatctggtt tgtcacctcg catatagcat taaatgcctc cgttgatggt
      301 agtctttcat agccacgacc accacaacgc tggcagtctc tgataacagg cataccacgt
      361 tttaccgact cttcacgatg aatggcgaca ccacgcccac ggcaatcctt acaggcggtg
      421 gaaacctcac cctttccgcc acactccgga caggcaactt ttaccacctc cctgactttt
      481 ttccattctt cccagtaaaa cggatacaca cctttcgtac actttgccca taccggcggc
      541 ttaccatccg gatactggat cttgtttgta aaaactatgc tttcaataaa tttttcccca
      601 tagcaacaag ggcactgctt tttactcgct gcgctgcggg cataatcctc aaaagcgtac
      661 gaagccataa tgcgcatcac taccggtttt atttctgccg gaagttttct caacgccgcc
      721 acacgatcgc accgactgag tgcataatct gccagtaatt ctgttgcccg cgccctgtca
      781 ttcatactga tgcccatttt ccccaggaac gcagaaaaac ccatctcagc ccgattctgt
      841 gtcatgccct gcgcggccat cacatcagtg atactcagcg catcttttga cgttgaggcc
      901 gatgcatcgg tcaggccagg ggattttggg gagtagtatt tcggtaaatc ttccagtttc
      961 attttttgac ctgctcttaa tgcattatgg ggtaaatctt cacccccaga cgtccaccag
     1021 atactggctg accacgaacg atattgattt catcaaactg ctcatcgtcc attaacactc
     1081 ccgcatgcgt cagcgcatcc agcggtgctt tcaggatatt gtccaggtcg cgacgacgct
     1141 tatccggtgg ctctgcaatc acctttatcg ccagccttcc ggacaggctt aatttcagcc
     1201 gctgctggcg aacaataagc gccacagccc ggcgataacg ctttccctcc tccgagataa
     1261 aatatgtgct gccacggcgt cgccagtaag tgttcaccgt cggcgggtaa ggtaaaacca
     1321 aatctatgag catcagtcac ctcttttacc caagcacgcc agttgcaaag gcgtgatcaa
     1381 gaaaacgaaa aattaaatca acctgagaac catgcttttc ttcgaacgcc agaggatccg
     1441 catgaagctc gttgtgatgc tcccgacaca gcggtagcgt gaaaatatcg tgagatttgg
     1501 tccccattcc gccctgacca tgaccaatca ggtgatgggg atcgtcggct ggcttaccac
     1561 aacacgcaca cggctgtgtc ttcacccagc gtttgtattt ctcgttaacc cagcggcgac
     1621 gtttaggtcg tttcatgaaa gattccggag actcaggatc aacggcaatg ctgaccaccg
     1681 tcttttcctg ttgtgg
//
LOCUS       DAJTQQ010000246         1639 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000246 DAJTQQ010000000
VERSION     DAJTQQ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.252"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            193..462
                     /locus_tag="OO309_004234"
                     /pseudo
     CDS             193..462
                     /locus_tag="OO309_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            459..685
                     /locus_tag="OO309_004235"
                     /pseudo
     CDS             459..685
                     /locus_tag="OO309_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            714..1304
                     /gene="ospG"
                     /locus_tag="OO309_004236"
     CDS             714..1304
                     /gene="ospG"
                     /locus_tag="OO309_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HCR5849121.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          480 a          337 c          386 g          436 t
ORIGIN      
        1 ctgtcagcgc cagtgatagg acaggtgaaa cctgtcaata cgtaccggct tacgccgggc
       61 ttcggcggtt ttactcctct gccatatgca acaacggagt gccgccttac atgccgctgg
      121 cgcggcatat cctggtgacg atatctgaac agttatatac tgtgtatata cgtggtaatg
      181 acgaggtggc aaatggcaca ggttaatatg agtgtaagaa tcgacgcaga actgaaggat
      241 gcttttatgg ccgctgcaaa aagcatggac cgtaatggct ctcagttaat ccgggatttt
      301 atgcgccaga ccgttgaacg gcagcacaat tcttggttcc gtgactaggt tgcggcaggc
      361 cgtcagcaac tcgaatgcgg tgatgtgctt ccccatgaca tggtcgaatc ctctgctgct
      421 gcatggcggg atgaaatgag caggaagatt gctgataaat gatggagatg ttctggacca
      481 tgctggccat ccaggacagg aagcgcattc gtagtacatt gcggagcaga acctgatggc
      541 cgccattgaa ccggacgaac ggattggtta ttcagccagc agcctggcag ggcagccgta
      601 taaaggccgc aacggtcgtg tggaaggaac atctggacca cacaaggtag cgtgtaacgt
      661 tattttgtgt gaaaatctct tgtgattttt aaatatcgta acaaaggtat gacatgaaaa
      721 taacatctac cattattcaa acaccttttc catttgagaa taataattct catgctggca
      781 tagtaacgga gcccattctc ggtaagttaa taggtcaggg gtcgacagca gaaatctttg
      841 aagatgtgaa tgattcatct gctttgtata aaaagtatga tcttattggc aaccagtaca
      901 atgagattct ggaaatggct tggcaagaat ctgagctttt taatgctttt tatggcgatg
      961 aagcatccgt tgttatacag tatggcggag atgtgtacct ccgaatgctg cgcgtgcctg
     1021 ggactcccct tagtgacatt gatacagctg atatccctga taatatagag agcctttatc
     1081 tacagttgat atgtaaattg aatgagttga gtataatcca ttacgatctt aatacaggta
     1141 atatgctgta tgataaagaa agtgaaagtt tattcccaat agattttcgc aatatttatg
     1201 ctgaatatta cgctgcaacc aaaaaagata aagagattat cgaccgacga ttacaaatgc
     1261 gtacaaatga tttttattcg ttattaaaca ggaaatattt atagacgtat ttgttgatgc
     1321 tataagccag aggctcttct atgagaatat ggggaccatt ccctcgcgat attaacgtaa
     1381 tatcttaatt gtatgcaccg ccagaatgcg ttcagtgcga tcacggcagc agacaggtaa
     1441 aaatagcaac aaaccacccg aaaaaccgcc gcgatcgcgt atgataaatt ttaaccacat
     1501 gcatacctgt gcaacaaggc gaatcacgca cggatttcca gccagagaca cctgaaacgt
     1561 aaccggctca tttaaaccgt ctggtctgtt acctccggtt ccacaaaaat aatgtccatc
     1621 attttgaagt ggacactat
//
LOCUS       DAJTQQ010000247         1613 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000247 DAJTQQ010000000
VERSION     DAJTQQ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.253"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..769)
                     /gene="ipaH7.8"
                     /locus_tag="OO309_004237"
     CDS             complement(<1..769)
                     /gene="ipaH7.8"
                     /locus_tag="OO309_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR5849122.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1098..1277
                     /locus_tag="OO309_004238"
                     /pseudo
     CDS             1098..1277
                     /locus_tag="OO309_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1294..1613)
                     /locus_tag="OO309_004239"
                     /pseudo
     CDS             complement(1294..1613)
                     /locus_tag="OO309_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          485 a          273 c          355 g          500 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggattgcaga acgcgagtcg
       61 acaatggatt ccctgaaata ttaatcctgt aacttgaatc taattgtgaa aaactctccg
      121 gaagagtggt tatctgatta aaatgaaaat aatattcctt ttgtctgata ttgtttctat
      181 cagaaaattg aggaaaagta accagattat tgcgctgagc acagagaatt tccagtttat
      241 ccggtaagtc gggtaatgtt gtcagcctgt tataataaac atgtaaaaat ttcaattttt
      301 cgggaagagc cggtaagtta tatagcctgt tattttcaac aaataatgat gttaaatggt
      361 ttggcaaaac aggaaggttt tccagttgat tagaacgaac acttaaagtt gttaatgact
      421 caggaagttc tggtatcaca gaaagcctgt tattatcagc attaagttct ttaagaaatg
      481 caggcagctt tggcaatgat gttaatttat tattttctac aatcaaagca gaaatatgct
      541 ttggtaattc gggaagagac gtcaaattta ggtctgataa atttaatact gcttcttgat
      601 tctgaaagca ctgggagagt ctgttaaaag caatgcctcg ctcttccccg ggagaagagt
      661 tcttttccca ttcagtcagg attctcagat aatgttcatt actggttgag tttgagttaa
      721 tagaggggga gcaagaaact gatgagtgtg tattatttac agagaacata atgctaggtc
      781 aaccttatat ttgtgagaat aaatatttaa aattgtatat ttatacaaat ttcacttacc
      841 atttccgata ttaagtttta atagtttcca ttttacacat aacagaagtt agttgtaatt
      901 tatttatgga ggttttctat ggaattcttt ttttattgtg gctgcagtca ataataacac
      961 tgaattcaac attccagtga gcaaggaaat gtaagccgag taagtatatt tgttttacat
     1021 catattttag tcaagctcat ttattcctat tatagaatga caaattaaaa ttactgtaaa
     1081 aaaacgatac acatttaata cgagggaggc cggcagaaaa acggcttgcg gtcaggcagg
     1141 agaaatgtgt gccgcgaatg aagtccttgt atgactggat aaagctccag aggaaaactt
     1201 tgtcaaacca tgctgaaatg gtaaaggcgt tcaactatat cctgaatcac tggaatgcac
     1261 tgaatgagtt ttgccgggta gtacttccaa tcattagaat atgtgctttt caatgaagga
     1321 accgatgact ttttcgtgga tttccacgga acgcgagaag cagattgttt tacgtgccag
     1381 ccgcttgatg cgggggcgga gggtcaggtt gttacgttca tacgttgcgt gaagattttg
     1441 ccggtcaggt gtttctcctc cggcacttct ctggcgtagc tgccccagtc atcgctggtc
     1501 accatgccga tacagaacgg agtcaacagg gctaacagtt cccgacaggt ctcatcattc
     1561 cgtggcccgc aagtgtaagc caacacaccg ccagttttag tgttgtacgc ata
//
LOCUS       DAJTQQ010000248         1604 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000248 DAJTQQ010000000
VERSION     DAJTQQ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.254"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..354
                     /locus_tag="OO309_004240"
     CDS             <1..354
                     /locus_tag="OO309_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849123.1"
                     /translation="PYPEYVISWQTLNPVSLHVSVTLKKSDYLPSDITRQVQQSVLDA
                     FNGTDGGLRARVASVVSAGRYYAGIYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEI
                     PVLDVSDISVKLQEA"
     gene            356..961
                     /locus_tag="OO309_004241"
     CDS             356..961
                     /locus_tag="OO309_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR5849124.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            948..1496
                     /locus_tag="OO309_004242"
                     /pseudo
     CDS             948..1496
                     /locus_tag="OO309_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647606.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          388 a          377 c          439 g          400 t
ORIGIN      
        1 ccgtatcctg aatacgtgat cagctggcag acactcaacc ccgtcagtct gcatgtcagt
       61 gtgacgctga aaaaaagtga ctacctgcct tcagatatta cccgacaggt acagcaatcc
      121 gtgctggacg catttaatgg tacagatggt ggtctgcggg caagagttgc ctctgttgtc
      181 tctgccgggc gctactatgc cggcatttac aaaaccgatc cggaacatat tgatattctg
      241 ggccttactg tgagccgtga tggttcgtca tggacaacgg ctgtcacttt cgggatagat
      301 gagattccgg ttctggatgt gtcggatatc agtgtgaaac tccaggaggc ataacgtgca
      361 gaatgtggct gccactgtgc ttgcgcagta tgctgccagc ccccgactca atgccctcat
      421 taacagcttt aacgcagcgc tttcccccga cagttttatc agtgattttt atggtcttat
      481 ctggaacatc gataccgcag aaaagtatgg tcttgatgtc tggggaaaga ttgtgggtgt
      541 cagtcgctgg ctgacggtaa aggacgattt taattacctg ggcttcagcg agtccaggat
      601 ggacaccccg gtaatggatg atccctgtcc gtttaatcag gcaccgtttt acaacggaaa
      661 atcggatacc cggactgttg acctgtctga tgctgtatac cggcggctga tactgatgaa
      721 agccatgtcg aacattactg actgttccgt tccggatatt aacctgatgc tgagatttat
      781 gttcggaaaa aaacgccggg cttatgttct gaataatggc gggctgagga tgagttacgt
      841 ctttgagtcc gcgctctcgt tggcagaact ggcgattatc cagtcgtcgg gtgcactgcc
      901 atccccgccg ggtgtttatg tttcagtagt tttaaaggag tcccgtaatg aaggccagtg
      961 ataaaccccg ccagctggcg gtcccctttg cgagtaccgg agataaaaac cgtatcccgg
     1021 acaaggcgac acagcagacc agagagagcg gtaatgctgc gtatgattca ggttttcctc
     1081 cggtgaccat gacagcggtc tcagcgggag gtataccgcc acacggcaag gattttaacg
     1141 gtctgatgta cgatattacc gcagcaatac ggttcgccca ggctggcggt ttgtacacgt
     1201 ataatgccgg ttttgcgggg gccattggtg gatatgcaaa aggagccatt ctcgccggag
     1261 tcgcaacaac agcggtctgg ctgaatacca cggacgataa cctgaccgat cctgaaggct
     1321 ccgacagtgc gggctgggta aatcttcttg aggatccgaa aaggatattc ctgcggcaga
     1381 agaacaatct gtcagacctt cagaataaag ggacggcacg ggataatctt caggtttaca
     1441 gtaaagagca gtcagatcaa cgctatgttc atcgggaagg cgataaaata accggagagc
     1501 tgaaaatccg tggtgttaat gcgctgagga ttttcaacga agcttttggc ctgatttttc
     1561 gtcgttcgga agagtgcctg caccttattc ccaccagtga aggt
//
LOCUS       DAJTQQ010000249         1551 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000249 DAJTQQ010000000
VERSION     DAJTQQ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.255"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            400..1149
                     /locus_tag="OO309_004243"
     CDS             400..1149
                     /locus_tag="OO309_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR5849125.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
     gene            complement(1319..>1551)
                     /locus_tag="OO309_004244"
     CDS             complement(1319..>1551)
                     /locus_tag="OO309_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5849126.1"
                     /translation="NGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGRLKEY
                     RRIATRYDKTARNYLAIVKLGCIRLFYQRLRN"
BASE COUNT          425 a          329 c          315 g          482 t
ORIGIN      
        1 ccataaacag ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa
       61 ggagtgtccg ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat
      121 atgttctact aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt
      181 ccccttgttg gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct
      241 ttttatgctt gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt
      301 tatttaaatt atgtgatagt acacacaatt ttattatcca gtactttatg ctcttaataa
      361 aataattata aatatgtatc ctctataagg agatgttata tgaaaagaca gctttttact
      421 cttagtattg tcggggtatt ttctttaaat acctttgcat cattccctcc gggaaatgat
      481 gtgacaacaa aacctgacct ttactacctg acaaacgata atgctattga cagtctggca
      541 ttattaccgc ctccaccaca aatcggaagt attgccttcc tgaatgacca ggccatgtat
      601 gaaaaggggc gtttattgcg aaatactgaa agagggaagc tggcagctga ggatgccaat
      661 ctcagtagtg gtggcgtggc aaatgtattt tctgctgctt ttggttctcc gataacggct
      721 aaagattcac cagaattgca taaattactg acaaatatga ttgaggatgc cggagacctg
      781 gcaacgcgtt cagcaaaaga atattatatg cgtattcgac cttttgcttt ttatggtgtt
      841 tctacgtgta atacaaaaga acaggataca ttatccagaa atggctctta tccatcaggt
      901 catacatcga ttggttgggc aacagcactt gtattgtccg aaataaatcc ggcacgccag
      961 gataccattc tgaaacgggg ttatgaactg ggggacagca gggttatttg cggttatcac
     1021 tggcaaagtg atgttgacgc agcacgtatt gttggctctg ctattgtagc aacactgcac
     1081 tcaaatcctg tgttccaggc acaattacaa aaagcgaaag atgaattcgc taacaatcag
     1141 aaaaaataat gcttttagcc agtacgcctt ataaccccga ccttcagggc taatgccagt
     1201 cagttaagca actgactggc tctttttcag ggctgtgggg tatttccagt gcctctcctt
     1261 taccactctc ggaaaggccc tttcccttct tgtcggtaat ttcactaggc tgtgtccctt
     1321 aattacgtaa gcgttgataa aagagtcgga tgcagcccag tttcactatc gccaggtaat
     1381 ttctcgccgt tttgtcgtaa cgcgtggcga tgcggcgata ttctttcagc cgcccaaagc
     1441 accgttcaac gacattacga ttgcagtacg catcacgatc aagctgtgca cgtccatccg
     1501 atgccatttt ctcatttgac tttcggggga ttactgcctt tataccgtta t
//
LOCUS       DAJTQQ010000250         1523 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000250 DAJTQQ010000000
VERSION     DAJTQQ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.256"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..379
                     /gene="ssuA"
                     /locus_tag="OO309_004245"
     CDS             <1..379
                     /gene="ssuA"
                     /locus_tag="OO309_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR5849127.1"
                     /translation="GVRVLKDGTDLNQTGSFYLAARPYAEKNGAFIQGVLATFSEADA
                     LTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKPVNAEVAALQQQTADLFYENR
                     LVPKKVDIRQRIWQPTQLEGKQL"
     gene            376..1521
                     /gene="ssuD"
                     /locus_tag="OO309_004246"
     CDS             376..1521
                     /gene="ssuD"
                     /locus_tag="OO309_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCR5849128.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
BASE COUNT          343 a          418 c          433 g          329 t
ORIGIN      
        1 cggcgtgcgg gtgctgaaag acggcaccga tctcaatcaa actggatcgt tttatctggc
       61 agctcgtccc tatgcagaaa aaaacggcgc ttttattcag ggcgtactgg caacctttag
      121 tgaggccgat gcgttaaccc gcagccagcg cgaacaaagc atcgctttac tggcaaaaac
      181 gatgggctta ccggcaccgg tgattgcctc ttacttagat catcgccctc ctaccaccat
      241 caaaccggtt aacgccgagg ttgccgcctt acagcagcaa acggcagatc tgttttatga
      301 aaaccgtctg gtgccgaaaa aagtcgatat tcgccagcgc atctggcagc ccactcaact
      361 ggaaggaaaa caattatgag tctgaatatg ttctggtttt taccgaccca cggtgacggg
      421 cactatctgg gaacggaaga aggttcacgc ccggttgatc acggttatct gcaacaaatt
      481 gcgcaagcgg cggatcgtct gggctatacc ggtgtgctga tcccaacggg gcgatcgtgt
      541 gaagatgcgt ggctggttgc agcgtcgatg atcccggtga cgcagcggct gaagtttctt
      601 gtcgccctgc gtcccagcgt aacctcacct actgttgccg cccgccaggc cgccacgctt
      661 gaccgtctct caaatggtcg tgcgttgttt aatctggtca caggcagcga tccacaagag
      721 ctggcaggcg acggcgtgtt ccttgatcat agcgagcgct acgaagcctc ggcggaattt
      781 acccaggtct ggcggcgctt attgctgggg gaaaccgtca atttcaacgg taaacatatt
      841 catgtgcgcg gagcaaaact gctcttcccg ccgattcaac agccttatcc tccgctttac
      901 tttggcggat cgtcagatgt cgcccaggag ctggcggcag aacaagttga tctctacctc
      961 acctggggcg aaccgccgga acttgttaaa gagaaaatcg aacaagtgcg ggcgaaagct
     1021 gccgcgtatg ggcgcaaaat tcgtttcggt attcgtctgc atgtgattgt tcgtgaaact
     1081 aacgacgaag cgtggcaggc cgccgagcgg ttaatctcgc atcttgatga tgagactatc
     1141 gccaaagcac aagccgcatt cgcccggacg gattccgtag gacaacagcg aatggcggcg
     1201 ctacataacg gcaagcgcga caatctggag atcagcccca atttatgggc gggcgttggc
     1261 ttagtgcgcg gcggtgccgg aacggcgctg gtgggcgatg gtcctacggt cgccgcgcga
     1321 atcaacgaat acgccgcgct tggcatcgac agctttgtgc tttcgggcta cccgcatctg
     1381 gaagaagcgt atcgggttgg cgagttgctg ttcccacatc tggatgtcgc cattccggaa
     1441 attccccagc cgcagccgct gaatccgcaa ggcgaagcgg tggcaaatga ttttatcccc
     1501 cgtaaagtcg cgcaaagcta agg
//
LOCUS       DAJTQQ010000251         1498 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000251 DAJTQQ010000000
VERSION     DAJTQQ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.257"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..559
                     /gene="curA"
                     /locus_tag="OO309_004247"
     CDS             <1..559
                     /gene="curA"
                     /locus_tag="OO309_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR5849129.1"
                     /translation="PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHA
                     DDFAEQLAKACPKGIDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGP
                     DRLPLLMATVLKKRIRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLEN
                     APQTFIGLLKGKNFGKVVIRVAGDD"
     gene            757..1422
                     /gene="mcbR"
                     /locus_tag="OO309_004248"
     CDS             757..1422
                     /gene="mcbR"
                     /locus_tag="OO309_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR5849130.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          445 a          312 c          370 g          371 t
ORIGIN      
        1 accagtgggg gcgacggtgg ggcaaatcgg caaacttaaa ggttgcagag tggtgggggt
       61 agccggtggc gcggaaaaat gccgccatgc taccgaggtg ttaggcttcg atgtttgcct
      121 tgatcaccac gcggacgatt ttgccgaaca actggcgaaa gcgtgcccaa aaggtattga
      181 tatctattat gaaaacgtgg gcggtaaggt atttgatgca gtgctaccat tacttaatac
      241 atctgcgcgc attcccgttt gtggcttagt gagcagctat aacgctacag agctaccacc
      301 cggtccggat cgtttacccc tgttgatggc aacggtgctg aaaaaacgta ttcgcttgca
      361 agggtttatt atcgctcagg attatggtca ccgcatccat gagtttcaga aggagatggg
      421 gcaatgggtg aaagaggata aaatccacta ccgcgaagat attactgatg gtttagagaa
      481 tgcgccacag acgtttatcg gcctgctgaa gggtaaaaac ttcggcaaag tggtgatccg
      541 cgtcgcgggt gatgattaaa gtttcaacgg cggcgtaagc cgccataaaa atccaggcaa
      601 aaagcttatt ttcagcttta attaactaac cacgtcattg ttctgctgct aataacaaca
      661 aaggtgggtg gtttatgatt attcacttta atacactagg tgaactcctt ctgccatgca
      721 ggcagggttg gacagaaaac acgcgaagga attaaaatgc ctggaacggg aaaaatgaaa
      781 catgtcagtt tgactctgca ggttgagaac gacctgaaac atcagcttag tattggcgca
      841 ttaaaacctg gcgcacgcct gattactaaa aatctggcgg agcaattagg tatgagtatt
      901 acacctgtgc gtgaagcatt attacgtctg gtttcggtga atgcgctttc tgtcgcacct
      961 gcacaagcat ttacagtacc ggaagtgagg caaagtcagt tagacgaaat caatcggatc
     1021 cgctacgaac tggaattaat ggcagttgcc ctggctgttg aaaacctctc ctcgcaagac
     1081 atagcagaac ttcaggaatt gctcgggaaa ttacagcagg cgcaagaaaa gggcgacatg
     1141 gaacaaatca ttaatgtgaa caggctattt cgcttagcga tttatcatcg ctcaaatatg
     1201 cccatcctgt gtgagatgat tgagcaactg tggatcagga tgggacctgc tttacattat
     1261 ctttatgaag cgattaaccc agcggaatta cgggagcgca tagaaaacta tcatctatta
     1321 ctcgctgcgt taaaagcaaa agacaaagag ggatgcaaac attgtcttgc tgaaattatg
     1381 caacaaaata ttgctatttt ataccagcaa tacaatcgtt aaattaaatg cccggcaaat
     1441 accgggcatc aagtaattac tcaaatcgcc acgcaatatt catgccgaca ccataatt
//
LOCUS       DAJTQQ010000252         1471 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000252 DAJTQQ010000000
VERSION     DAJTQQ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.258"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            197..448
                     /locus_tag="OO309_004249"
     CDS             197..448
                     /locus_tag="OO309_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849131.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            515..793
                     /locus_tag="OO309_004250"
                     /pseudo
     CDS             515..793
                     /locus_tag="OO309_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            795..>1471
                     /locus_tag="OO309_004251"
     CDS             795..>1471
                     /locus_tag="OO309_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5849132.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLP"
BASE COUNT          358 a          375 c          385 g          353 t
ORIGIN      
        1 ccctcgccac ctctgatgtg gcaggcatcc tcaacgcacc cgcacttaac ccgcttcggc
       61 ggttttttgt ttttattttc aacgcatttg aagttctgga tggtgccgga atagaatcaa
      121 aaatacttaa gtagcgcgca gggataaaag ggatggaccc cgaacagggg agtgctactt
      181 atctggaagg attctgatga tgaacatcga agaactgcgt aaaatttttt gtgaagatgg
      241 cctctatgct gtgtgcgttg aaaatggaaa tattgttagt cattaccgca ttgtgtgttt
      301 gcaaaaaaat ggggctgcgt taattaattt tgtggatgcc cgagtgacgg acggatttat
      361 cttgcgcgac ggtgagtttg tcacttcatt acaggcattg aaagagatcg gaataaaagc
      421 tggcttttct gctttttcag aagaataaac tcatctacaa tcttgcgcgg ggctgaactc
      481 ccgctgagta acaccgtgcc accggagaaa accgatggca cgcaacgcaa aatattacaa
      541 tcatgataat tcgaccgttc ttgcccacac gcacgagcgg tattctcacg catttaagtc
      601 agactggtac cagcatccct catgcactga agaacaggcc gaatggctta ttcagtgtta
      661 ccgcaggcgc ggatgcgagg ttaaaaaagc ccttagcctc gactaccgtc actagataat
      721 ctccgtcagg ctcccttact ccgaacagcc accgcgtccg tcccgcacat tccagcaacg
      781 gatctggagg taacgtgcgg gtattacttc gacctgttct ggttccggaa ctcgggctgg
      841 tggtccttaa gcccggtcgt gaatccatgc cggtattcca caatacccgg gtactggtgg
      901 agccggaacc gaaaagcatg cgtaatctgc cgtccggggt cgttcctgcc gttcgccagc
      961 cgctagtgga agacaaaaca ttgctgccgt ttttcagtaa cgcacgggtg attcgtgctg
     1021 ctggtggtgc tggcgcattg tctgactggc tgttgcgcca tattaaatcc tgccagtggc
     1081 cacacggcga ttatcatcac agcgaaaccg tcattcaccg ttatggtacc ggcgcaatgg
     1141 tattgtgctg gcactgcgac aaccagctgc gtgaccagac atccgaatca ctcgagcaac
     1201 ttgctcatca aaacctgtca gcatggatga ttgacgtcat cggtcacgca ataagcggta
     1261 cgcaggagcg tgaattatct ctggctgaat tatcctggtg ggcggtccgc aatcaggtgg
     1321 cggacgcgct accggaagcg gtattacgtc gttcgctggg gttgcgtgcg gaaaaaatcc
     1381 gctcaatgta ccgtgaaagc gacatcgtgc cgggagagca gaccgccacc agcatactga
     1441 agcagcgcac aaaaaatctt gcgccgctgc c
//
LOCUS       DAJTQQ010000253         1443 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000253 DAJTQQ010000000
VERSION     DAJTQQ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.259"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            82..1275
                     /locus_tag="OO309_004252"
     CDS             82..1275
                     /locus_tag="OO309_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HCR5849133.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
     gene            complement(1295..1443)
                     /locus_tag="OO309_004253"
                     /pseudo
     CDS             complement(1295..1443)
                     /locus_tag="OO309_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          422 a          314 c          339 g          368 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac cattccatca gtaccgacaa aatgccctga tggatgccac aatggcgtta
      181 atcaacggag catctctgac gcagaccagt atcggacgtt ttttgccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt ggtcttcggg ccagcaaaag tcgttcaaca
     1021 ggaagaattc tggttctgag tctgctggca acgttgagca ctatcgtaat gtggttactc
     1081 ggttatcacg ctgaaaataa agggttacac ctgaaatatc aggctaacag cattaaatcc
     1141 cggcgggtta tctcatatct gacgttagcg aagaatgttc ttcgacactc gccacttatt
     1201 ttaagacgaa cagtactgag cacagttcta aaccatctgt ccagaaccta ccgaaatatg
     1261 gtgctggttt attagcagta attttgtggg gatccctcag cctgaagggg cgacacagtc
     1321 ggggagagaa gcccatctcc ttgccgaact gccacagcga aggatggaac cggtgctgac
     1381 cggtctgata tgcgtcacgt tgcagaacca cagttttcat attgtcatac aacacttcgc
     1441 gcg
//
LOCUS       DAJTQQ010000254         1440 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000254 DAJTQQ010000000
VERSION     DAJTQQ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.260"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..1132
                     /locus_tag="OO309_004254"
     CDS             join(<1..750,750..1132)
                     /locus_tag="OO309_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR5849134.1"
                     /translation="LDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKLSFL
                     KDAIRSRWMWNMRQRLLKAWSEGLAMPESLSHITTESQRRSLVLKAGGKYWHVYMSKK
                     TAGGRNTARYLGRYLKKPPIAASRLAHYNGGGSLSFRYLDHKTGETATETLTQRELVA
                     RLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLS
                     RDPFECVLCGGRMVYRRAIAGLNVEGLKKNARDISLLRYMPA"
BASE COUNT          336 a          357 c          464 g          283 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ctgagcttcc
      481 tgaaagacgc gatacgttcg cggtggatgt ggaatatgcg gcaacggctt ctgaaagcgt
      541 ggtcagaggg gctggcgatg ccggagtcgt tgtcacatat cacgacggaa tcacagagga
      601 gaagcctggt gctgaaagcc ggaggaaaat actggcatgt gtacatgtcg aaaaaaacgg
      661 ccggcgggag gaatacggcg cgctacctgg gtcgttatct gaagaagccg ccgatagcgg
      721 cctcccggct ggcacattac aacggagggg gagcctgagc ttccgttacc tggaccacaa
      781 aacgggagaa acggcgacgg aaacgctgac acagcgtgag ctggtcgcga ggctgaaaca
      841 gcacatcccg gagaagtttt ttaagatggt gaggtacttc gggttccttg ccaaccgggt
      901 gtgtggagag aagctgccgc aggtgtaccg tgctctgggg atggataaac cggaaccagt
      961 ggcgaaagtg tgctatgcac aaatggtgaa acagttcctg agtcgtgacc cgttcgaatg
     1021 cgtgctgtgt ggcggccgga tggtataccg ccgggccatc gcgggactga atgtggaggg
     1081 gctgaagaaa aacgcgcggg atatcagtct gctgaggtat atgccggcct gacacaggct
     1141 aagtgtgccc gggaatcggt gcaagtataa aaaacagcct gttgcggtat aagaatcgac
     1201 catgcagaga gagaaaacac cggagtggag ggaaaagcag aaaagctcac gtggcataag
     1261 gcgtggacag cggtatcgtc tggtttttca atttcctata cgtgaacctg ggtgccggcc
     1321 ccggacggga ccgacggttt ctgggatgtc gggtgtctgc ccctgaaata cctcacactt
     1381 ccgtccccgc ccggcagctt acttccgtgc tgtcgggtag cagcgattca ttactgacag
//
LOCUS       DAJTQQ010000255         1419 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000255 DAJTQQ010000000
VERSION     DAJTQQ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.261"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            40..>1419
                     /locus_tag="OO309_004255"
     CDS             40..>1419
                     /locus_tag="OO309_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR5849135.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKIEGHEWVAGNVNGDKGNS
                     LKVNLIGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDARR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRVVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIVSR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGKACPLNEPILLADGTWTTHGNV
                     KIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
                     RRVIDTFGLK"
BASE COUNT          364 a          300 c          421 g          334 t
ORIGIN      
        1 tgtcgatttc tggtttaaaa atctcaatct gagggaatca tgaggccaag tgaacttagc
       61 gacctgcttt gggcgcaggt tgacagggtg gctccgcacc tgttgccaaa cggcaagata
      121 gaggggcatg agtgggttgc cggtaacgtc aacggtgaca agggaaacag ccttaaggtc
      181 aaccttatcg gcaagaaaaa atgggctgat ttcgctgagg gagacggcgg tgacatgctt
      241 gatttgtgga tggcatgtcg tggaattaac ctgcatcagg ctatgcagga agcgaaagca
      301 tttctcggta tcaaggatga cgatcaccat ttcgatgcca gacgtgagaa gaaattctcc
      361 agacctgatc gtaagaaaat cgcccgctac gttaccagaa cagaatccca tcttgagtac
      421 ctgcaatcgc gtggcatatc gccagaagtc gtaaagcgct acgaggttgt cagcggcaag
      481 gtgtggaatg gagaacgaga actggatgct ctggtgcttc cgtacaaacg cgatggtgag
      541 ttgttgcagg tcaagcgaat cagcactgag cgcccggacg ggaagaaagt cattatggca
      601 gaaggtgatt gcgaaccttg cctgttcgga tggcaggctc tggacgctgg cgtgagggtg
      661 gttgtgcttt gcgaaggcga aattgattgt atgagctatg cgcaatacgg catctcggcg
      721 ttatccgtgc cgtttggtgg cgggaaaggc gctaagcaac agtggattga gtttgagtat
      781 cacaacctcg acaggtttga ggaaatattc atctcgatgg acgttgatga tgttggtcgt
      841 gaagccgcaa gggaaatcgt aagccgactc ggtgaacatc gttgccgtct tgttactctg
      901 ccgtacaaag acatcaacga atgcctgatg aacggtgtta ccgaggatga aatctggcag
      961 tacatcggca cggcatccta cttcgatcct gaagaactct acagtgcgcg agagttttac
     1021 caggacacta tcaacgcttt ctacggcaag cagcagtatc tgtttaatcc accgtgggaa
     1081 tctctggcag ataaattcca gttccgtgag gcagagttga cgctggtcaa tggtgtgcac
     1141 ggtcacggaa aggcatgccc actgaatgag cctattcttt tagctgatgg gacatggact
     1201 actcacggga atgtaaaaat tggcgatcag gtggcgtcag tagacggcaa tccgtcaact
     1261 gtcactggga tattcccgca gggtgttaga gatgtttacc gagtcacatt tgaagatggt
     1321 cgttatgttg attgcgcagg cgatcaccta tgggaggtca ctagtcgtgg attcacgaaa
     1381 ggcgagaaac gccgcgtgat agataccttc gggttgaag
//
LOCUS       DAJTQQ010000256         1411 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000256 DAJTQQ010000000
VERSION     DAJTQQ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.262"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..456
                     /locus_tag="OO309_004256"
     CDS             <1..456
                     /locus_tag="OO309_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5849136.1"
                     /translation="NIQAGTARKFTLSSANNSTKNAAFYLWGNPSRPVVAELGDDSGW
                     HFFSQRNPDNSIVFTVNGQVIPLNYGNFDARYKYRTEGVQDVRYGHEMYYSPGSNTVS
                     WRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKLINGTWYNVASI"
     gene            459..1004
                     /locus_tag="OO309_004257"
     CDS             459..1004
                     /locus_tag="OO309_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5849137.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFS
                     TITDKSSYNAIEWPASPEGSS"
     gene            1028..>1411
                     /locus_tag="OO309_004258"
     CDS             1028..>1411
                     /locus_tag="OO309_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5849138.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVMAG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTA"
BASE COUNT          396 a          311 c          381 g          323 t
ORIGIN      
        1 aatattcagg ccggaactgc cagaaaattc acgttatcca gcgccaacaa ctccacgaaa
       61 aatgcagcgt tttatttgtg gggtaatcca tccaggcctg ttgttgcaga gcttggtgat
      121 gattcaggct ggcatttttt cagccagaga aacccggata acagcatagt gttcactgtt
      181 aacggacagg taattccgtt aaattacgga aacttcgatg cccgctataa atatcgaaca
      241 gagggggtac aggatgtacg gtatggccat gaaatgtatt acagccccgg cagtaacacc
      301 gtttcgtgga gattttgcgc accttcggga cacgggctgt cagggatggc gatatcggat
      361 accggccgta actcagcgga taacgttgac ggtgtgtatt accgaccact gcaaaaactg
      421 attaatggca cctggtataa cgtagcgagt atttaacaat gttgcattta aaaaatatta
      481 ctgcgggtaa tccgaaaaca gcagaacaat atcagatgac aaaacgatat tcggtcacct
      541 ggcttttttc agaagacgga aaaaactggt atgaagagct gaagaatttc gccagcgaca
      601 caataaaaat agcttacacc ggagatggtc gcgtggtgtg ggtcggtaag gatgtgacag
      661 gcatcgagcc acgcaatgcc agcgttatcg aagttccgga tattaccgct aaccgacgga
      721 ttaccgcgcc gggttactgg ttttaccgca atgatgaatt tgtctttgac tacagactca
      781 aagcggaaga tgagcgtgat gcccttctgg ctcaggtcag tgcccggaca ggggaatggg
      841 aagaagacct gctgctgggg ttaatcagcg acgaagataa agaaaagctg aaagcctgtc
      901 gtatttacgc gaaatcgctg caggcgatgg atttcagcac catcactgat aaatcctcat
      961 acaacgccat tgaatggccc gcctctccgg aaggttcttc ctgatttaat ttatcgcgag
     1021 aaaaacaatg tctgtagtga tatcaggtgc gctgactgat ggcgcaggta tccccatgtc
     1081 cggataccat attattctga aatcccgggt aaacaccccg gaagtggtga tgaacactgt
     1141 tgctgatgtg atggcaggaa acgatggtga atactgtttc catgcgcgga ctggaaaata
     1201 tggtgtgtat ctgaaacagg actggcgcaa cgagtacaac gttggcgaca ttgctgtata
     1261 tgaggactca aagcccggca cgctgaatga ctttctgatt gctcctgatg agggcgacct
     1321 gaaaccggat gtcgtcaaac gctttgagga aatggtggcg caggcgcagc agagcgccgg
     1381 ggccgcagcc ggaaacgcac agcagacggc g
//
LOCUS       DAJTQQ010000257         1391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000257 DAJTQQ010000000
VERSION     DAJTQQ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.263"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..154
                     /locus_tag="OO309_004259"
     CDS             <1..154
                     /locus_tag="OO309_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCR5849139.1"
                     /translation="TPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRMKERR
                     KEWGTL"
     gene            139..777
                     /locus_tag="OO309_004260"
     CDS             139..777
                     /locus_tag="OO309_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCR5849140.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1005..>1391
                     /locus_tag="OO309_004261"
     CDS             1005..>1391
                     /locus_tag="OO309_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849141.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          405 a          252 c          344 g          390 t
ORIGIN      
        1 tacacctcac ccgtcctggc gacgtatctg taagacactc ataaagaatg atttctggtg
       61 ccggactctc tccttcagtc cgaacaaacc ccggcactat gaacgttacc tgcagcgtat
      121 gaaggaaagg aggaaggaat gggggactct gtgacaccgg agatacttgg caacatgatt
      181 cgtcagtatt tcagtcaggt acgaactgaa aaagagagca tacaggctct gaatcatctg
      241 cgcagagtgc ttcatgaggt cagtcctttt gcacaggagc ctgtggactg tgtgttgtgg
      301 gtgaaagcgg atgaggtggt ggccaacgat tacaacccta atgtcatggc actaggtgag
      361 aaaaaactac tgaaacagtc tctggagaaa gatggtttca cacaaccggt ggtggtgtca
      421 gaggagaaga atcattatct ggtggtggat ggttttcatc gtcagttact ggggcgggaa
      481 gcagatgcag gaaaacgcct gaggggctgg ttgcctgtag cctgcattaa tccggagaga
      541 aagggacagg ccgcacgtat tgccgcgacc attcgacata accgggccag ggggaaacac
      601 cagatcactt taatgtctga tattgtcagg gatctttcac gtctgggctg gacgaatgaa
      661 cgtattggta cagagctggg aatggaccag gatgaagtgc tgaggctgaa acagattagc
      721 gggctgacag agttgtttca ggaagaggat ttcagctcgg cttggacggt tcgttgatgt
      781 tgttaaatat gggcggtatt tctgaaagtt gatactttgg gtagaaaaaa tgataccgaa
      841 aactatggag gatgtgcttg tcaggtgact ggacaccata ggtcttaggc tttgataacg
      901 ggataagatg agcggctttt ctcataccat caggtgtgat gatggcgcag atttacaaaa
      961 tctgattgat tctaaagatg tataagtaaa aatcaagctt tgatatgaaa aaacagatat
     1021 ttattaataa taaacctcct gtagtccctt actcagggac gcacgcgaaa atatttaaat
     1081 acattgaaat ccctctgccg tttttctact ttatctatac atcaggcgaa ccattccaca
     1141 tatctgtcca aaatactgtt atttatgtat caaaatacaa tggtatattt attaataaat
     1201 tagttccttt ttctctttta tttgacaggg atatatctgt tcttcaaaga agagatattt
     1261 gtgttgtgcg atttacgtca gaggaaataa gtgaacataa cgttttattt gatcatgaca
     1321 ttgaacgatt aaaaaaaatt tccaaagctc agcttatatc tcccgattat gttttgattg
     1381 atttttctag t
//
LOCUS       DAJTQQ010000258         1332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000258 DAJTQQ010000000
VERSION     DAJTQQ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.264"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..>1332)
                     /gene="btsS"
                     /locus_tag="OO309_004262"
     CDS             complement(<1..>1332)
                     /gene="btsS"
                     /locus_tag="OO309_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR5849142.1"
                     /translation="IVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLV
                     GLTGGLHRYSMGGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFV
                     AEMVQMLIILAIARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSA
                     FSATALKVAASTEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDD
                     HHLPGKPISSTYTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVM
                     GTIKLYEAKNRLFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNP
                     HFLFNALNTIKAVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQI
                     EKARFQSRLQVNIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAI"
BASE COUNT          330 a          352 c          334 g          316 t
ORIGIN      
        1 gattgccact cgccctgtat ccagcagttg tgatgtccca tgtttaatgg cgttttccac
       61 tatcggttgc agggtaaacg cgggcaattg ctgctgggat aattcttgcg gaatagcaat
      121 gttgacctgc aaccgcgact ggaagcgcgc cttttcaatt tgcagataag cgttcacatg
      181 ttcaatttcg tcggcgagag taacaaactc cgaaggccgc tttaagtttt tgcggaaaaa
      241 agtggaaaga tactgcacca gctggctggc ctgttcgctg tcgcggcgga tcaccgcttt
      301 aatggtgtta agcgcattaa acaaaaaatg aggattcacc tgggcgtgaa gcagtttgat
      361 ttctgactgg gtgagcatcg ctttttgccg ctcatattgc cctgcaagaa tctgcgccga
      421 aagcagttgc gcaatcccct cgcccagcgt gcggtttatt gaactgaata accggttttt
      481 ggcttcatac aatttgatgg tgcccatcac gcgctgattt tcgccacgca acggaattac
      541 cagcgtcgac cccagtttgc attgcggatg caaagagcaa cgataaggta cttcgttgcc
      601 atcagcgtag accacttcac cggtttcaat cgcttttaag gtgtaagtcg aagaaatcgg
      661 tttgccgggt aaatggtggt cgtcaccaat tccggtaaag gccagcaatt tctctcgatc
      721 ggtaatcgcg actgcaccaa tatccagctc ctgatacagt acctgagcca ctttcatgct
      781 gttcacttcg ttaaacccct gtcgcaaaat gccttccgtc gaggctgcca ctttcagcgc
      841 agtggcagaa aaagccgaag tgtatttttc aaacatcgcg cgtttatcga gcaatatacg
      901 cataaacagc gccgcgccga cggtattggt gaccatcatt ggcgcagcaa tattactcac
      961 cagacgcacc gcatcttcat aaggtcgggc gatcgcaagg atgatcagca tttgcaccat
     1021 ttcagcgacg aacgtgacgg caccggcggt aatggggtta aagactttat cagtgcgccc
     1081 gcgacggatc aggatgctgt gtaccaggcc gccgagcaat ccttcaacga tggttgagat
     1141 catgcaactt agcgcggtca tgccccccat cgaatatcga tgtaagccgc cggtcagacc
     1201 aaccagccca ccgacgaccg gaccgccgag taagccgccc attaccgcgc ctatcgcacg
     1261 ggtattggca atagaatcgt caatgtgcaa cccaaaccag gtgcccatga tgcagaagat
     1321 ggaaaagacg at
//
LOCUS       DAJTQQ010000259         1313 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000259 DAJTQQ010000000
VERSION     DAJTQQ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.265"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..>1313
                     /locus_tag="OO309_004263"
     CDS             <1..>1313
                     /locus_tag="OO309_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5849143.1"
                     /translation="ETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQMVNNELAAK
                     KALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAENLTSLTLS
                     NGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGVGELSSSSL
                     KRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDNIWASLQNA
                     QIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADALAADRQLFSSPA
                     DITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTVKEQNEPVA
                     QVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYSVSSRLSVDPQAK
                     DISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQQN"
BASE COUNT          389 a          308 c          316 g          300 t
ORIGIN      
        1 agagacatat caactttcga aacgtctgtg gcagcaaatt accgtgcaaa gtctggcgga
       61 aaaatggcag cagttaagaa agaaccagga cgcattttgg gagcaaatgg taaacaatga
      121 gcttgccgcc aaaaaagcat tgtctcctgc ggctatcctg gcgctggaaa aagagtacgc
      181 caacaaaaag ctggcggctt acatcttccc aggcagaaat ttatcgttaa cagtagacgc
      241 tgacccacag gcggaaatta gcagcaaaga aacgctggct gagaatctga catcattaac
      301 actttccaat ggtgccaagg ttattctggc aaaatccgcg ggtgaagagc aaaagctaca
      361 aattattgcc gtatcgaata aaggcgattt aagtttccct gcgcagcaaa aatcacttat
      421 cgcgctggca aataaagcag ttagcggaag cggcgttggc gaactctcct cttccagcct
      481 gaaacgctgg agtgcggaaa actcggtaac catgagcagt aaagtcagtg gcatgaatac
      541 gttgctctcc gttagcgcgc ggactaataa ccctgaacct ggtttccagt tgattaacca
      601 gcgaatcacc cacagcacga ttaacgacaa tatttgggca tcgctacaaa atgctcaaat
      661 tcaggcgttg aaaacgctcg accagcgtcc agcggagaaa ttcgcccagc tgatgtatga
      721 gacgcgctat gctgatgacc gcacgaaatt actgcaagaa aatcagattg cacagtttac
      781 tgccgcagat gcgctggctg ccgatcgcca gttgttttca tctccagcgg atatcacgtt
      841 tgtcattgtc ggtaatgtcg cagaagacaa actcgtggcg ttaattacgc gttacttagg
      901 ctcaatcaaa cactctgatt cgccattagc cgcaggtaaa ccattaactc gcgcgacgga
      961 caacgcatcg gttactgtaa aagaacaaaa tgaacctgtg gcacaggttt cacagtggaa
     1021 gcgttatgat tcccggacac ctgttaatct ggcgacgcgt atggcgctcg atgcttttaa
     1081 cgtcgcactg gcaaaagatc tacgcgttaa tattcgtgaa caggcatctg gggcatacag
     1141 cgtttcttct cgcctctcgg ttgatcctca ggctaaagat atcagtcatt tgctggcttt
     1201 tacttgtcaa ccagaacgac atgatgaaca gttaacgtta gcgaatgaag tgatggttaa
     1261 gcgtttgact aaggggatca gtgagcaaga actgaacgaa taccagcaaa acg
//
LOCUS       DAJTQQ010000260         1301 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000260 DAJTQQ010000000
VERSION     DAJTQQ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.266"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OO309_004264"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OO309_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR5849144.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSSLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          364 a          234 c          292 g          411 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttgtt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag tgagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 cccctcacta ttcatatctt ggtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actatctcga tattcccata gtaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtcgtg agccgttttc agacaatcgc a
//
LOCUS       DAJTQQ010000261         1288 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000261 DAJTQQ010000000
VERSION     DAJTQQ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.267"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            522..806
                     /locus_tag="OO309_004265"
     CDS             522..806
                     /locus_tag="OO309_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCR5849145.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            806..1081
                     /locus_tag="OO309_004266"
     CDS             806..1081
                     /locus_tag="OO309_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR5849146.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          339 a          322 c          325 g          302 t
ORIGIN      
        1 acagaataag tttacgcgta aatcgccctg aatccagaag agcattaagt atcacataaa
       61 caggttccat accagtcctg accgcatcag gacgccagta aataaaatct cgtagtccac
      121 cggcatcaga gacaagagta atcgtcgtta ccggtatttc gactggtttg tactggtgtg
      181 agcggacaaa actactgtac gtctctcata cacgctcgta agcggccctc acggcccgct
      241 aacgcggaga tacgccccga ctgcgggtaa acccttgtcg ggaccactcc gaccgcgcac
      301 agaggcattt tcatcgctga caggccgcat ggcctcgcag agccggaggg ggactggtga
      361 tattcggacg accgtaccgg cttgcgccgg gcttcggcgg ttttacacct gtgctacatg
      421 taaccaccgt atgccgcctt atatgcgctg actcgcatta tttgccttgt tccgtctgaa
      481 agtaatcact atgattaaat atgattaaca actaatcggg tatgcaaatg aaaaacaata
      541 ccgcacaagc aacaaaagta attaccgcgc atgtgccatt acctatggct gataaagtcg
      601 accagatggc cgccagactg gaacgctccc ggggctgggt tatcaaacag gcgctttctg
      661 catggcttgc ccaagaggag gagcgtaatc gcctgacgct ggaagccctg gacgatgtga
      721 catccggaca ggttatcgac catcaggctg tacagtcctg ggcggacagc ctcagtactg
      781 accatctgtt accggtgcca cgctgatgga actgaagtgg accagtaagg cactttctga
      841 tttggcgcgg ttatatgatt ttctggtgct ggccagtaaa cctgctgccg ccagaacggt
      901 gcagtccctg acacaggcgc cggtcattct gttaactcat ccacgtatgg gagaacagtt
      961 gtttcagttt gaacccaggg aggtcagacg gatttttact ggcgagtacg aaatccgtta
     1021 cgaacttact ggccagacta tttatgtatt gcgcctgtgg cacacacgag agaacaggta
     1081 gattgggtgt ggaggcaact ttaacatagc ctgcaggtat taattctgga gcattgttat
     1141 caggccagat attttgcatt ccattaaata atcttgtagg agataaaata aattacgatg
     1201 aaataacgaa aattaccgct cgtgactggc ggcaatatcg cgctccgggc tggcaaataa
     1261 cccatcagaa acgttattgc cagacatt
//
LOCUS       DAJTQQ010000262         1275 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000262 DAJTQQ010000000
VERSION     DAJTQQ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.268"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            34..435
                     /locus_tag="OO309_004267"
     CDS             34..435
                     /locus_tag="OO309_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5849147.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="OO309_004268"
     CDS             447..818
                     /locus_tag="OO309_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5849148.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..>1275
                     /locus_tag="OO309_004269"
     CDS             805..>1275
                     /locus_tag="OO309_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5849149.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNPPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIP"
BASE COUNT          303 a          286 c          396 g          290 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atccgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccg
//
LOCUS       DAJTQQ010000263         1244 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000263 DAJTQQ010000000
VERSION     DAJTQQ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.269"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            199..>1244
                     /locus_tag="OO309_004270"
     CDS             199..>1244
                     /locus_tag="OO309_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR5849150.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          405 a          283 c          197 g          359 t
ORIGIN      
        1 tggtataaat cctctgcatt gtgaatatgt aaaacatcaa aaaaagatga accaaatata
       61 cgaaaaaagc aaatatactt accagggtag aaagcatgat ataacactca ttcccttatg
      121 gcaaatattc tgaaacaccc tcctcattag gagagataca ttaaatacaa tttattcaca
      181 atctaattcc atatccatat gcgagaaatt aatatgctca gaaacatttc atcctgttta
      241 tttccacata tcagcacaat tacatccccc aaccattatt tgtccgaatg ggatgattgg
      301 gagaaacagg ggttaccgga agaacagcgt actgaggcgg taagaagact tcgtgcatgt
      361 cttacctcta aggggcataa actggacctg cgagccttgg cgctttcctc gctacctgta
      421 ctccctgctt gcattaaaaa gcttgatgtg agctgtaata aattaaccat ccttactgat
      481 ttacctgaaa atattaaaga acttattgca agagataatt tcttaacaca tatatctgca
      541 ttaccacatt atctaataac tttggatgtg tccgaaaatc aattagagaa tctgccgtta
      601 ttaccagaca ccatcaaatc actaagcgca gagtataata ggttatccac actgccttca
      661 ttacccttga atttaaaaaa acttgaggtt aggaacaacg aactgcaaac tcttccatct
      721 ctgccttcta atcttaagat acttaaggtt gcgcacaacc atcttactga actgccccct
      781 ttacctagga gactgcaact tctttttgca tatagcaata gattaagcaa cttaccaaac
      841 atccaagaaa atattatcat gagaagattt ttttattttg aaaacaacca aataactaca
      901 atcccgacaa atctttttcg tttagatcct catataacta ttgagattgc aaataacccc
      961 ttatcagatc aaactctgct attcttaata cagcaaactt cggttccaaa ttttaacggg
     1021 cctcagtttc gtatttccct gtcagaccaa aacagactgt ttttacgcca gatgttgccg
     1081 caaaatttac attcgcgcca tatcagagtc atcactgaag gggggcagaa ctttcagatc
     1141 ccccctcttc ccgaaactgt ggcagcctgg tttcctgaag cagatcgtcg ggaggtttct
     1201 acacaatgga cttctttttc caccgaggag aattcccggg catt
//
LOCUS       DAJTQQ010000264         1232 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000264 DAJTQQ010000000
VERSION     DAJTQQ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.270"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..585)
                     /locus_tag="OO309_004271"
     CDS             complement(<1..585)
                     /locus_tag="OO309_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR5849151.1"
                     /translation="MKISLVVPVFNEEEAIPIFYKTVREFEELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHTTGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVR"
     gene            complement(582..944)
                     /locus_tag="OO309_004272"
     CDS             complement(582..944)
                     /locus_tag="OO309_004272"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol glucosyl transferase"
                     /protein_id="HCR5849152.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYAAHTNQAMANFAGFVV
                     AVSFSFFANAKFTFKASTTTMRYMLYVGFMGTLSATVGWVADRCSLPPIVTLVTFSAI
                     SLVCGFVYSKFIVFRDAK"
BASE COUNT          359 a          273 c          245 g          355 t
ORIGIN      
        1 gcgtacatat tcaacgacat ctgtctgacc accaacccag ctcagtatgc ctttcatgaa
       61 aagattgcgc tcaggcaata gtttaatgtt ctccacaacc tcacgagaca tgagtcgaaa
      121 atctccgaca ttttcctcta tttttggggt gcttattttg ttgtgtaatt tatagaacca
      181 ctcagctgtc ttacgtttca gccgtccatc agttgagcgg tctgaacgtt tagcaagcac
      241 catgtcagca cctgcctgcc acttttcaat aagatgaggg ataacctcaa tcgggtcttg
      301 caggtcaaca tcaattggaa tcacggcatc gccggttgta tggtctaacc ctgcaaacaa
      361 tgctggttct ttaccaaagt tgcgtgtaaa tgacagcgga acaactagcg gatcagaaac
      421 agccagagcg ttaatgattg actccgtagc gtctttgctt ccgtcattta tgaaaacaat
      481 ttctacttca tatggcttca actcttcgaa ttcacgtacc gttttataaa aaattggtat
      541 cgcttcttct tcattgaaga caggaacgac aagagatatc ttcatttcgc atccctaaag
      601 acaatgaact ttgaatagac gaaaccgcac accaagctga tggcggagaa ggtaacaaga
      661 gtgacaattg gaggaagtga acatctatca gcaacccatc caacagtagc actcagtgtt
      721 cccatgaacc cgacatataa catgtagcgc atcgttgtag ttgatgcttt gaatgtgaat
      781 tttgcattcg caaagaagct aaaactcaca gccacaacga aacctgcgaa gtttgccata
      841 gcctgattgg tatgcgcggc atagatacat acaccaaaaa ccacccagtg tataagtgtg
      901 ttcagcacgc ctatagatgt gtactttgca aatagcttta acatttcttc tatcagctaa
      961 taatcaaagg catgaagtct atcatccaag tctcaatcga tcgatacttg cggtagttga
     1021 tgagaaaaac tctggcacac aaatctttgc actggattgc aaggctttgt gctcttctgt
     1081 gaatgtgtgg ctatatgttt gaagatcgtt gtgccgtatt tgtgacatat atatggcaac
     1141 atcattcatc aactttctgt ttgtgccatc aactatatct tagtgaatgc ggttaatgct
     1201 tgctaaaaca gatagttatg attggtgcta ca
//
LOCUS       DAJTQQ010000265         1191 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000265 DAJTQQ010000000
VERSION     DAJTQQ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.272"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(1..264)
                     /locus_tag="OO309_004273"
                     /pseudo
     CDS             complement(1..264)
                     /locus_tag="OO309_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(379..1017)
                     /gene="ospI"
                     /locus_tag="OO309_004274"
     CDS             complement(379..1017)
                     /gene="ospI"
                     /locus_tag="OO309_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR5849153.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
BASE COUNT          353 a          291 c          183 g          364 t
ORIGIN      
        1 cagcaagcgc tgtaactctt ttatttgttt tatagcggca gccagttctg aggcaggaac
       61 gacatcctct ccggcagcaa cagcagtgag acttccttcc agatattatt ttttccactt
      121 aagcaacagg ctgggctgaa taccatgcaa acgcgccaca tgggacaccg tcatgccagg
      181 ctccattgtc tgctgaataa tggcaatttt ttcctgagga gttttacgtt tacgaagctc
      241 cagccccagc aaagttccag tcatctcaaa attagaacta gtgttagaca tatatccaag
      301 cctatctctt attttaagat acatccactg tctggcgttt caaggggccg gctcaaatct
      361 atgtacaggc tctcagcatc agcaaagcct cttacttttc caactactac tgttagcata
      421 gtgatcaaat tcctgatcag atacatgaat tacctgaacc gaactatttt catctacaaa
      481 tgcatctggc gatttttcta caggatcata aagattaaat ccctttcctg tttgaacatc
      541 caaaaacata attttttcac catcgttaaa tccagctatc caatgagtgg cctgttcaat
      601 agaaatcatt actacacttc cttttgaagt attttgtaaa atattttctg caatacgctc
      661 acgaaattta ctataagttt cattaggatg tgacactatg ccttcactat caatttgctc
      721 taaatgccta tgaaaaaatc cggtcatata ctctgatatt tcattaggta cgggagcctc
      781 tcttactcca taccctaaca tagccataca agcatgaagc gcacaccctg aacagttacc
      841 atccccacca gacggattta tcattttctg taaagtcgct gccaattcag aatctttatc
      901 catatcacca agacttttag agtttaaaga ttccagcaat tttttaacat ttacatttcc
      961 ctcctctgta tgtgcctcat atccagcagt tccctgtaac gacaccccat taatcatatt
     1021 gtatcctgac agtatatata ctcactatat tttcttcata acaaatatcc ctctgatatg
     1081 acaacaaaaa acggagccgg attcctgttt ttgtgaactg cagctattcg tgtctaaaaa
     1141 gtgatccact taccaccaac ggcctaatat atacagaacg gagtaaacag g
//
LOCUS       DAJTQQ010000266         1142 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000266 DAJTQQ010000000
VERSION     DAJTQQ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.275"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..137)
                     /locus_tag="OO309_004275"
     CDS             complement(<1..137)
                     /locus_tag="OO309_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5849154.1"
                     /translation="MGSTRVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSK
                     AP"
     gene            complement(305..877)
                     /locus_tag="OO309_004276"
     CDS             complement(305..877)
                     /locus_tag="OO309_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HCR5849155.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          362 a          234 c          208 g          338 t
ORIGIN      
        1 ggcgctttgc ttttatcctc tttattaaat ggatctaccc aagcctggat gagataaggt
       61 attttttctt ctttgctaaa tacagaaata gatttatcag cattattact taagtaaacc
      121 acccgcgtac taccaatgac aacgcctgca taagttaatg ctggttggcc taaaaaaacg
      181 ctgagtataa tcgcggtaaa taacttacat taaactgttg ttttaaagag cctgattaaa
      241 caactttttc tggacataac gactccctgg ccaaagccaa ctgaataaaa aggcccttga
      301 taaattattt cttatattcg acagtaaatt gtgaatcagc gttcgccgta ccagccgtta
      361 cggtcgcatt ggttgccaca tagcgtgcaa taaacttcat ttcagccttg ccttcagcat
      421 cgatagcggt ataagcagag tcgttataaa gtttaacctg cgaattatcg gactggttaa
      481 aaatacgaat accaacgccg gttgccgcaa tcgcttgtcc tgtaccggta aaatcaccag
      541 ggttgcctgc tgtaagcggc gtacccactt tcaggtcgcc gtcgccgcca ggcgcttctg
      601 taccatcaaa acgaacggct gcttgagtgt acgttgccgg gcaattttca agtttaatac
      661 tgaacttctg tggcgacgcc agactaccta cacctgaaaa tgcattttta ctgactttac
      721 ccagtgggac aatttgatcg gctgtatcgg aggtaacttc acaagtagtg tcgacaattt
      781 caccgacgaa gttaacaaca ccatcttctg ccagtgcatg cccggccagc attagagatg
      841 ccagagtgaa tgcaccgcct ataatattac gcttcataaa aactccattg atataatata
      901 tcctttacca tgaatcatca cttatattaa taagtgtgtt tttgattcaa aggaatagta
      961 caccaaagag acggctttat ctaaaaataa tattaattac gcaaaaataa aatatagatt
     1021 tattgtttaa atattattta aacagtaaaa aatcaagttt gttatcagac attaatgata
     1081 attcagaata tatttatata gaaagataga tgaatttatt gatgtcagtt ataaaaaagg
     1141 gc
//
LOCUS       DAJTQQ010000267         1140 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000267 DAJTQQ010000000
VERSION     DAJTQQ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.276"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            41..616
                     /gene="ssuE"
                     /locus_tag="OO309_004277"
     CDS             41..616
                     /gene="ssuE"
                     /locus_tag="OO309_004277"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR5849156.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            609..>1140
                     /gene="ssuA"
                     /locus_tag="OO309_004278"
     CDS             609..>1140
                     /gene="ssuA"
                     /locus_tag="OO309_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR5849157.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNV"
BASE COUNT          271 a          310 c          289 g          270 t
ORIGIN      
        1 tgttaacaaa aaaaagaaca attggttata aggagagagt atgcgtgtca tcaccctggc
       61 gggtagtcct cgctttcctt ctcgctccag ctccttgctg gaatatgcgc gggaaaaact
      121 aaatggccta gatgtagagg tttatcactg gaatctgcaa aacttcgccc cggaagatct
      181 gctttatgct cgtttcgata gtccggcact caagaccttc accgaacagc tgcaacaggc
      241 tgatgggctg attgtcgcca cgcctgtgta taaagccgcc tattccggcg cgctgaaaac
      301 cctgctcgac ctgctgccag agcgcgcttt gcaaggcaaa gtggtgctac cgctggcgac
      361 gggcggtacc gtggcccatc tgctggcggt cgattatgcc cttaaaccag ttttaagcgc
      421 actgaaagct caggagatcc tgcacggcgt gtttgccgat gactcacaat taattgatta
      481 ccatcacaga ccccagttca cgccaaatct gcaaacccat cttgataccg cgctagaaac
      541 tttctggcag gcattgcacc gccgcgatgt tcaggttcct gaccttctgt ctctgcgagg
      601 taatgcccat gcgtaacatc attaaactgg cgctggcggg attgcttagc gtctctactt
      661 ttgcggttgc tgcagaatcc tcgcctgaag cgttacgtat aggctatcag aaaggcagta
      721 ttggtatggt gctggcaaaa agccaccagt tactggaaaa acgctatccg caatcaaaaa
      781 tctcctgggt ggagttcccc gcgggtccgc aaatgttgga agcgttaaac gttggcagta
      841 ttgatctcgg cagtaccggg gatattccgc caatctttgc ccaggctgcc ggggctgatt
      901 tggtatatgt gggcgtcgag ccaccgaagc ccaaagccga agtgattctg gtggcagaaa
      961 acagcccgat caaaaccgta gccgatctta aaggtcacaa agttgccttt cagaaaggtt
     1021 ccagttcaca caacctttta ctgcgtgcac tgcgtcaggc cggacttaaa ttcaccgata
     1081 tccagcccac ttacctgacg cctgccgatg cccgcgccgc gttccagcaa ggtaacgttg
//
LOCUS       DAJTQQ010000268         1101 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000268 DAJTQQ010000000
VERSION     DAJTQQ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.277"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            1..1009
                     /locus_tag="OO309_004279"
                     /pseudo
     CDS             1..1009
                     /locus_tag="OO309_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          310 a          270 c          258 g          263 t
ORIGIN      
        1 caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg aagagtttta
       61 ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac ccgaatggca
      121 gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga taaccgctcc
      181 tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac gtgagtttgc
      241 cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg cggatgagtc
      301 ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg acgataaacc
      361 atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt acgactggaa
      421 agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc cactgtataa
      481 cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac cttttggccc
      541 ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct gggctgctat
      601 gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg acaatcattt
      661 ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat atgcattatt
      721 gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa tcgccattta
      781 tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag gggacattgg
      841 cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata acgattactg
      901 gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt ataacgaacg
      961 gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac gcaggattta
     1021 acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa tgaaatccgc
     1081 atggcgatcc atcaggttag c
//
LOCUS       DAJTQQ010000269         1080 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000269 DAJTQQ010000000
VERSION     DAJTQQ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.278"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..271)
                     /locus_tag="OO309_004280"
     CDS             complement(<1..271)
                     /locus_tag="OO309_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5849158.1"
                     /translation="MTTVTVHCPRCNSDEVYRHGRSCSRHERFRCRSCKRVFQLTYSY
                     EARKLGVKEQIVEMAHNGAGGRDTARTLKIGINTVIRTLKSSRPGG"
     gene            194..445
                     /locus_tag="OO309_004281"
     CDS             194..445
                     /locus_tag="OO309_004281"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849159.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAII"
     gene            complement(465..>1080)
                     /locus_tag="OO309_004282"
     CDS             complement(465..>1080)
                     /locus_tag="OO309_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR5849160.1"
                     /translation="GIVWRRAAPTLRIRDPHKDEKMAAIHKALDECSAEHPVFYEDEV
                     DIHLHPKIGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVSYVGGNSKSSALFIS
                     LLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWVNHVERL
                     WQALHDTITRNHQCRSMWQLLKKVRHFMETVSPFPGGKHGLAKV"
BASE COUNT          247 a          275 c          262 g          296 t
ORIGIN      
        1 atccgccggg gcgagagctt tttaatgtcc gtatgacggt attgatgccg attttcagtg
       61 tcctggcggt atcacggccc cctgcgccgt tatgtgccat ttcaacgatc tgctccttaa
      121 cgccgagttt tctggcttca taagagtaag tgagctggaa aacacgttta catgagcgac
      181 aacggaaacg ctcatgccgg gaacaactac ggccatgtcg ataaacttca tcggaattac
      241 agcgcggaca atgaacagta acggtagtca tggaaaacct caaataccag tagattacca
      301 gaaacttcta tcgattgaag acattacctt ttgccgcaat aggtggggta acattggtga
      361 aaacgcatta cgccgggtgg cagttggaaa aaaattatct tttttcggct ctgatcgtgg
      421 tggagaaaat gcggcaataa tctaaatagc tgcgcttaat accgctacac ttttgccagc
      481 ccatgtttgc ctccggggaa tgggctgacg gtttccataa aatggcgaac ttttttcaac
      541 agttgccaca ttgagcggca ctgatgatta cgcgttattg tgtcgtgaag tgcctgccat
      601 agccgttcaa catgattcac ccatggcgag taaaccggct gataaatgcc cctgaacttc
      661 gggttctcct tcagccagct ctgtgtttcc cggcttttgt ggataatgta gttgtccacg
      721 atcagcgtga tggttttcgc acgacggtat gtcgctttaa gccgcttcag caggctgatg
      781 aacagcgccg aacttttgct gttgccgccc acatagctga ctttacctgt cccgctgtgc
      841 agcgctccgg ccagataata tttttcattc tgtcccggcg tgaccacccg tttttgctgt
      901 ccgcgcagtt gccagtccgc accgattttg ggatgaagat ggatatccac ttcatcttca
      961 taaaagaccg gatgctctgc gctgcattcg tccagtgctt tatggattgc tgccatcttt
     1021 tcatctttat gcgggtcacg gatacgcaga gttggcgcag cccttcgcca cacaatcccc
//
LOCUS       DAJTQQ010000270         1079 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000270 DAJTQQ010000000
VERSION     DAJTQQ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.279"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            136..441
                     /locus_tag="OO309_004283"
     CDS             136..441
                     /locus_tag="OO309_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849161.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            441..791
                     /locus_tag="OO309_004284"
     CDS             441..791
                     /locus_tag="OO309_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5849162.1"
                     /translation="MEPYSLTLDEACHFLKISRPTAINWIRTGRLQATRKDPTKNKSP
                     YLTTRQACIAALQSPLHTVQVSAGDGITEERKCHSSAEVKYGTPVSHCRTVKDLNSLL
                     EQRTKGRRQNSMTS"
BASE COUNT          357 a          236 c          264 g          222 t
ORIGIN      
        1 ctggcgttct ctctggtaca cctggggaca aaaagaaacc ggagaatggc agtggacatt
       61 tcaggtcggg gaccttgaaa acgtcaatat cactcactgg gcagtaatgc caaaagcacc
      121 ggaggctgga gcataatgac cacttttacc gacaaagaac tgattaaaga aattaaagag
      181 cgtatcagca gccttgacgt gcgagacgat attgagcgcc gtgcttatga aatcgcactc
      241 ctatctctgg aagtagaacc agatgaacgc gaagcttatg aattattcat ggaaaagcgt
      301 tttggtgact tagtagatcg tcggagagca aaaaacggcg ataacgaata catggcatgg
      361 gatatgactc tcggttggat cgtctggcag caacgagctg gtatccattt ctcaacaatg
      421 tcacagcaag aggtgaaata atggagccat acagcctcac actcgatgag gcctgtcatt
      481 ttctcaagat atccagaccg actgccatta actggatacg cacagggcgt cttcaggcaa
      541 cacgcaaaga tcccactaag aataaatctc cttacctcac aacacgacaa gcctgcattg
      601 cggctcttca gtctccgctg catactgtcc aggtgagcgc gggtgatggc ataacagagg
      661 aaagaaaatg tcactcttcc gcagaggtga aatatggtac gccagtttca cattgccgaa
      721 cggtaaaaga tttaaacagt ctcttggaac aaaggacaaa aggcaggcga cagaactcca
      781 tgacaagcta aaggctgaag catggcgggt cagcaaactt ggtgaaatac ctgatataac
      841 gttcgaggaa gcgtgtgtca ggtggcttga agagaaagca cataaaaaat cactggacga
      901 tgacaaaagc cggatcggat tctggcttca acatttcgca ggaatacaac taagagacat
      961 tactgaatca aaaatttatt cagcaatgca gaaaatgacg aaccggcgtc atgaggaaaa
     1021 ctggaaactc agggcagaat catgcagaaa aaaagggaaa cctgttccag aatacacgc
//
LOCUS       DAJTQQ010000271         1078 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000271 DAJTQQ010000000
VERSION     DAJTQQ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.280"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(176..>1078)
                     /locus_tag="OO309_004285"
     CDS             complement(176..>1078)
                     /locus_tag="OO309_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849163.1"
                     /translation="LEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQETE
                     IEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANLAL
                     AEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQAAA
                     IVELAEATHKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEPMK
                     SIDGIKIIQVDGLNSGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNEIG
                     VSGGSLAALTSPLISTTPVAENVK"
BASE COUNT          208 a          291 c          267 g          312 t
ORIGIN      
        1 ctccccgatt gttgatgagc gccggacctc gcgcaacgct ttcccagagg cagttttgcc
       61 ttccccgagc ggtcacgcaa aacgttgcaa cgacctgatc atttgcagtt tttagcggaa
      121 cgcagatata aaaaatcccc ggctttttgt gccagggacg ttcatagctg aaaatttatt
      181 ttacgttttc ggcgacggga gttgttgaga ttaagggtga agtcaatgcc gccagtgaac
      241 cgcctgaaac gccaatttca ttgagcaagg agtcaatcag cggagcctgt gtgcggtaag
      301 agagagcggc tgataatgct tgttccgcta ggtttccgcc ccccacatta cccgtgtttg
      361 catcacccgc agcgctgcca ctattcagac catcgacctg aataatcttg ataccgtcaa
      421 tagatttcat cggctcaacg gatttttcta ttaccgcagg tagcgcctgc aacaaggcca
      481 gtttgaattt aaggctggtt tgttcatcag aaagtacgtt gatagcatcg ttcagcgcac
      541 gttgtgcttc tgcttccgcc agaccctttt tatgtgtagc ttcggctaac tcaacgatag
      601 ccgccgcctg catctctgcg gcttcttttt ctgctttcgc ccgcacggtc agttcaaccg
      661 ctttggtttc tgcatcctgc gcggcagcga ttagggcaac ttgtttagca cgatcggctt
      721 ctgcggtctg gcgagtggtt tctacgtttt gttgcgcact tactgcttct gcgagtgcaa
      781 ggttagcacg cgcttctgcc tgggactgtt gttccgattt ggcggcaata gcgatcgatt
      841 tcgtctggtt ggcgatttcg gtgacctgct gctgttcgat ctctttaata cgaacttcac
      901 gttcagcctc aacctttctt gagcggacgg cctgctcgcg ctcgatctcc gtttcctgaa
      961 tttgtcgttc agccagaatt cgcgtctgct ctgcttcacg acgacgttca gcttcaaaag
     1021 ccgcaatacg tgcattctgt tcggcagtac gggttttaac ctgctgctcc tgctcaag
//
LOCUS       DAJTQQ010000272         1054 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000272 DAJTQQ010000000
VERSION     DAJTQQ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.283"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..796)
                     /gene="dkgB"
                     /locus_tag="OO309_004286"
     CDS             complement(<1..796)
                     /gene="dkgB"
                     /locus_tag="OO309_004286"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR5849164.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPE"
     gene            complement(959..1035)
                     /locus_tag="OO309_004287"
     tRNA            complement(959..1035)
                     /locus_tag="OO309_004287"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(999..1001),aa:Asp,seq:gtc)
BASE COUNT          276 a          247 c          237 g          294 t
ORIGIN      
        1 attcaggagc cagaccttcc gggctaacca ggcggtcgtt gcaatccagt gcggcgatcg
       61 cttttttatc ttcggcatca agctgtaaat tttgtgcctt aagattactt tccaggtttt
      121 tacgtttagt agaagaagga attactgagt aaccttcccc catagcccac gccagaatca
      181 cttgtgccgg aatcgcattg tgtttagctg caatacgagc aataacctca tctttcagtg
      241 ccttaccata agccagcgtc atataggaag tgatatgaat accgtgctgt ttagcccagg
      301 caaccacttt acggttttgc agataaggag agagttcaat ctggttggta gcgatgtttt
      361 cagcgccaac agcagcaatc actttttcca tcaatgggat cgtgaagttg gaaataccga
      421 tctcacgcgt cagcccttgt tttttggctt ccagcagcgc ctgcataaac tcttcaacag
      481 agacttcatc gtttggtgac ggccagtgga ttagcgtcag atcaacataa tcggtacgca
      541 atttttgcag gctctctttc agactcggga tcaatttgtc tttgctgaga ttttcaatcc
      601 agattttagt ggtgatgtag agttcatgac gtggcacgcc actttctgca atcgcctgac
      661 ctactgcggc ttcgttatca tagatttgtg cggtatcaat tgcgcgataa ccaagttcaa
      721 gcgccgtttt cacagatgaa ataacaacgt cgtctttcag acggaaagta cctaaaccaa
      781 atgcagggat agccataata tgcctctttt aatgctattt tcgttaactg aggtgattat
      841 gggcttcaga ctgttgcggg aaaagagcgt aaaacgcaga ggatttttgc gattctagca
      901 ataatagata tccagacgca aaaaaacatc gagcgttaac tcgaggcttc ttaataagtg
      961 gcggaacgga cgggactcga acccgcgacc ccctgcgtga caggcaggta ttctaaccga
     1021 ctgaactacc gctccaccga attcttttac aacc
//
LOCUS       DAJTQQ010000273         1052 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000273 DAJTQQ010000000
VERSION     DAJTQQ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.284"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            1..218
                     /locus_tag="OO309_004288"
                     /pseudo
     CDS             1..218
                     /locus_tag="OO309_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706798.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            364..>1052
                     /locus_tag="OO309_004289"
     CDS             364..>1052
                     /locus_tag="OO309_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5849165.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWV"
BASE COUNT          257 a          251 c          327 g          217 t
ORIGIN      
        1 aggcacaccg agcagggaat tctggttatc ggtggggctg ttcgccagaa aaaatttgac
       61 ccgttgtcag ttgatctgcc cgactggttg tcagaaacac tctggcatga gtgggtccag
      121 ttcaggcagg cattgcgaaa accgattcga acggagctgg gcgctaacgg ggcgatacgg
      181 gaactggaaa aattccgtca gcagggtttt acacctgagc aggtgattcg acacagcatc
      241 gccaatgaat accagggtct gttcgcgccg aaaggtgttc ggcctgagac gttgctccga
      301 caggttaaca ccgtctcgtt gccggacagt gcgatcccgc caggcttcag ggggtaacag
      361 accatgaaaa atattgcgac aggaggcgtt ctggagcgta tccgcagact gaccccacca
      421 catgtaaccg ccccattcag aacggttgcg gagtggcgcg agtggcaact tgctgaaggc
      481 cagaaacgtt gcgaggagat caaccgcctg aatcgtcagt tgcgggtgga aaaaatcctg
      541 aaccgctccg gcatccagcc gttgcaccgg aagtgttcgt ttgcgaatta ccgggcgcag
      601 aacgacggtc agcgacatgc actgagtcag gcgaaatcca tagctgacga actgatgaca
      661 ggctgtacga attttgtgtt cagcggtaag cccggcaccg gaaaaaatca tcttgcagcc
      721 gcgattggca accggctgat ggcgaagggg cgtagcgtga ttatcgtcac cgtgtccgat
      781 gtcatgagcg tgttgcatga gagctacgac aacggcaaat ccggtgaaaa atttttacag
      841 gagctttgtg gtgttgacct gctggtcctg gatgaaattg gcatgcagcg ggatacgaaa
      901 aacgagcagg tggtactgaa ccagattgtt gatcgccgga cggcatcgtt acgcggtgtg
      961 gggatgctga caaatattaa ccatgcagcg atgaatacac ttctcggcga gcgggtgatg
     1021 gatcgcatgg tcatgaacgg cgggcgctgg gt
//
LOCUS       DAJTQQ010000274         1050 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000274 DAJTQQ010000000
VERSION     DAJTQQ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.285"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..107)
                     /locus_tag="OO309_004290"
     CDS             complement(<1..107)
                     /locus_tag="OO309_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCR5849166.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILL"
     gene            complement(173..412)
                     /locus_tag="OO309_004291"
     CDS             complement(173..412)
                     /locus_tag="OO309_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCR5849167.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(547..>1050)
                     /locus_tag="OO309_004292"
     CDS             complement(547..>1050)
                     /locus_tag="OO309_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5849168.1"
                     /translation="ENGYRFLLLLLQGESDKQHIINEVWREQKGSVSDSSYYGQIYML
                     RKALDVAGLSSSLIKTIPRKGVRYLGKVEKHVCENERDSDEDSSSYTNDSEAREVNTD
                     ANIPENDNSQEFSTEELLPIKHTDKNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVL
                     LFLNQDK"
BASE COUNT          306 a          239 c          176 g          329 t
ORIGIN      
        1 agtaatatat ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact
       61 cttagtaacc atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga
      121 catattcctt tacgtctgac agcctctaga gagttttttt attttttata agttacggat
      181 tactcgttcc ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt
      241 taattgcgcc caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca
      301 tagcaacacc aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac
      361 gcgcaaagca ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct
      421 cgtatattat tatttaaatt caaaccacca gcttcatctg cgtgtcaccg gtatcccata
      481 tttttgggat gtggctataa ttatctattc ccgctattta gtcttcaaaa aaaattacat
      541 ttaatatcat ttatcttgat taagaaatag taagactaaa aaaacaagcg tcgtcagcca
      601 gcacactgct aacacggtaa gaacgctaat aaaaacactc cactgcttcg ttctatacca
      661 ttcaatattt ttgtcagtat gttttattgg gagcagctct tcagtagaga attcctggct
      721 gttatcattc tcaggaatgt ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt
      781 atagctacta ctgtcttcat cactgtcacg ctcattctca cagacatgtt tctcgacctt
      841 tcccagatat ctgacgcctt ttcgcggaat ggttttaatt agagaactcg acaaacctgc
      901 gacgtctaat gcctttctaa gcatgtaaat ctgcccataa taactgctgt cgcttaccga
      961 acctttctgc tcccgccaaa cctcattgat aatatgttgc ttatcggatt ctccctgtag
     1021 taaaaggagt aaaaatcgat agccgttttc
//
LOCUS       DAJTQQ010000275         1037 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000275 DAJTQQ010000000
VERSION     DAJTQQ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.286"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
BASE COUNT          305 a          238 c          220 g          274 t
ORIGIN      
        1 tttgttgatg cggtgcctgg tgcctccagg tgacgttaac cagttaacaa ttaacgccgg
       61 atacagggaa acccataacc acccgatacg tgggaactaa tcgcttttta actgttccgc
      121 gtgcgctcag ccgcattcac cgcatcacaa aattcacttt aaaaagggcg gacatcagcc
      181 agcaatgaaa ctgatgccgc caaaggtacc aatcaacatg gagtgttgtg gcgtggttgt
      241 cacttaagcg tatggtcaac ctgacaaccc ggtgtcctca acggggaagg aataactccg
      301 ccatacttac cgccgcgtca tttcgcgttg tgtgcctgct tttaactacg tcaggcgagg
      361 tggttcctgt tattccccaa caacaagaaa tttgtataat ctgaataccc caacaataag
      421 aagagtgagc aagtgatgta ttagcgatgt cagctctcaa agagactgca tcaatgttaa
      481 aattattcaa tgaagcaaaa actcaggctg aggttgatcg cgctgtgtct gaaataaact
      541 ctaagttgtc aagcattcag cttgaatatt tgtcatctca ttgatgagct gacaatctct
      601 aaaagcaatg aggctgcgct caaagcaaaa ctcacggaga tagaaaagtt taagttccaa
      661 agtgaagact gcgaacctcg ctaattgatt actggaacga ttgtgtatgc cagacagtgc
      721 aatactagct ctggcaagat taccatgtat ttttgtccag aatgttttgc gaagagagca
      781 atatctatat tgcaaccttt ccctatcgag gaaggcgata aatatacgaa aactcaatgc
      841 ccctcctgta aaaatgagta tctcatggac tacaatttca ataatgattt ttgagtcacg
      901 gtattcagat ttttaaagaa catgccggat actcacccgt gtccggcgca cgcactccac
      961 ctcacccgtg gagaactcct taattaccaa ccttggcttc gttggttagc cattaacgct
     1021 ggtatgtaat cattctg
//
LOCUS       DAJTQQ010000276         1030 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000276 DAJTQQ010000000
VERSION     DAJTQQ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.287"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            148..423
                     /locus_tag="OO309_004293"
     CDS             148..423
                     /locus_tag="OO309_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849169.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            523..>1030
                     /locus_tag="OO309_004294"
     CDS             523..>1030
                     /locus_tag="OO309_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR5849170.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDE"
BASE COUNT          349 a          216 c          229 g          236 t
ORIGIN      
        1 accaacgacg gaaatgaaaa aatattgtag tgaaatctgt gccgaaaaag accagatggc
       61 acacgaactt taattaactg actatccgaa actgaattta tgccagcaat ggcagggatt
      121 cgctcaacct taattaagga gaaaaacatg attaccagtt atgaagccac tgttgtaact
      181 actgatgaca ttgttcacga ggttaatctg gaaggaaagc gtattggcta cgtgattaag
      241 acagaaaata aagaaacccc attcactgtg gttgatatcg acggtccatc aggcaacgtt
      301 aaaacactta acgatggtgt aaaaaaaatg tgtctggtgc acataggaaa gaatctgccc
      361 gcagaaaaaa aagccgaatt tctggcaact ctgattgcaa tgaaattaaa aggtgaaatc
      421 tgaaaaaaag aaagcctgca cactgtgcag gcctgaatga agaacctggg acatttattc
      481 atcactcgca gtaattttaa tctgagttga ggttaaaaaa caatgaacac cgataaacaa
      541 gtttacccac tgtattacga agcaaaaaat gacaaagtaa gaaaacgtct cggtattaaa
      601 ggcggttttt actgggctga agcgaaaaaa ttatccattg ccatctcccg tggtgctgtt
      661 gcgattgacg atgcaggcta cgatgaagat gactttaaaa aacctgttcg cgtcaatttg
      721 cccgttgttg atgacctccc gccagaaggc gtatttgata cggaattctg caaccgttac
      781 gaaaaaggcg gggaagatgg catcacaatg gtatttatcg catcttctcc ctctgttcag
      841 gacaaaccgg ccagcactga caataccaac gtcaacggcg aagacatgac agaaattgag
      901 gagaacatgc tcctgccagt ttccggtcag gaactgccca ttcgctggct tgcgcaacac
      961 ggcagcgaaa aaccagtaac gcacgtttca cgcgacgaac tccaggcatt acacattgct
     1021 cgggatgaag
//
LOCUS       DAJTQQ010000277         1001 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000277 DAJTQQ010000000
VERSION     DAJTQQ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.288"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(142..478)
                     /locus_tag="OO309_004295"
                     /pseudo
     CDS             complement(142..478)
                     /locus_tag="OO309_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          332 a          181 c          178 g          310 t
ORIGIN      
        1 agaacttctt cagcacactg ctaaaacgca tagtgatcga gcgctgattc tggcgaacaa
       61 ctgaactaat acatcagaat ctgcattatg ttaaataaat atgaaaagat ggtttaaata
      121 ccccgttact tttgacttac actatacggt atcgcatcgt ttaatattcg caccggccag
      181 atttttattt ctattagttg tcacaatact gaatgcgtac gaccacagta ttctggctcc
      241 tgtgtggtta tgctttaatt ctgcgttccg ggcagataag cagttgcttg caggaatcct
      301 tcttgtgtta atgtcagttc ccctttttac cagtgctgat ttccacattc cgtccaacag
      361 agcttatagc ctttccctgg attatagcat tgtccggctg aagttctttt tgaataataa
      421 tagaagcact gctggcagat ccagtccgtt tttcataacc cactgtactg ataaccataa
      481 tctaatcagt agaaattgag tcgaaaacaa gcactactct atacaggata attagaggtc
      541 agtttgatta ttcacaattc atcatcagca ttttctattt ctgacgaaat caacatgaaa
      601 ataaccatat atgataatta ttataataac ggctttaatt ggaatacata tattacaacg
      661 tattatatat aattggtatt ctgggaacta tattctcaaa atacagtaga aacgaggtat
      721 gtttctggtg gaaaggacag tgggattaaa aagtaagaat tgataaaaaa aacgccagca
      781 acacatagtg ccggcggagg gaatacccca tggagaaaat gtgatgccta gagcatcatg
      841 aatatataat taaaaatagt tagcgttgtc aatactttca caaaaataat tttcgtagta
      901 tagaaagata tttttatgca tgacctacct gaatttgctc cgggtagagg ttataaataa
      961 aaattgaatc acgacaaaca caatattcat agtatggcga t
//
LOCUS       DAJTQQ010000278         1000 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000278 DAJTQQ010000000
VERSION     DAJTQQ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.289"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..591
                     /locus_tag="OO309_004296"
     CDS             <1..591
                     /locus_tag="OO309_004296"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HCR5849171.1"
                     /translation="YTVTLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDP
                     LVLTFGKVEPRPNTVNVVPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGI
                     DIDLWMDEEPVPMNKELVATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIP
                     SINGISHNPAERTNITDLAEGVKTLALMLYQLAWQE"
     gene            602..>1000
                     /locus_tag="OO309_004297"
     CDS             602..>1000
                     /locus_tag="OO309_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HCR5849172.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYK"
BASE COUNT          274 a          239 c          247 g          240 t
ORIGIN      
        1 tatacggtga cgctgaacgg cgaatcaaac catgcaggca ccacgccgat gggttatcgt
       61 cgtgatacgg tttacgcttt cagtcgcatt tgccatcagt cgatcgaaaa agcgaaaaag
      121 atgggcgatc cgctggttct gacctttggc aaagtagagc cgcgcccgaa tacggtaaat
      181 gtggtgccgg gtaaaaccac gttcaccatt gattgtcgtc ataccgacgc tgccgtgctg
      241 cgcgatttca cccaacagtt agaaaacgac atgcgggcga tttgcgatga aatggacatt
      301 ggtattgata tcgatttatg gatggacgaa gaacccgtgc cgatgaataa ggagctggtc
      361 gccaccctga cagaattgtg tgaaagagaa aaactgaatt accgggtgat gcacagtggt
      421 gccgggcacg acgcgcaaat tttcgcgcct cgcgtaccaa cctgcatgat ttttatcccc
      481 agcatcaacg ggatcagcca taacccggcg gaacgcacca atattactga ccttgccgaa
      541 ggggtcaaaa cgttggcact catgctttat caacttgcct ggcaggaata aggagtcata
      601 aatgggatat ttaaataacg tcaccggtta ccgcgaagat ttactggcta accgtgcgat
      661 tgttaaacac ggtaatttcg cactgttaac cccggatggt ctgataaaaa atattattcc
      721 ggactttgaa aattgtgacg cgacaatcct ctccacgcca aagctgggtg cctcttttgt
      781 tgattatctg gtcacactgc atcaaaacgg tggcaaccaa cagggcttcg gtggcgaagg
      841 cattgaaacg ttcctgtatg tgatctctgg aaatatcact gccaaagccg aaggcaaaac
      901 atttgcctta agcgaaggtg gctatcttta ttgcccacca ggctccttaa tgacgtttgt
      961 taacgcccag gccgaagaca gccaaatctt tttatataag
//
LOCUS       DAJTQQ010000279          990 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000279 DAJTQQ010000000
VERSION     DAJTQQ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.291"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(107..826)
                     /gene="yehT"
                     /locus_tag="OO309_004298"
     CDS             complement(107..826)
                     /gene="yehT"
                     /locus_tag="OO309_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR5849173.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(823..>990)
                     /locus_tag="OO309_004299"
     CDS             complement(823..>990)
                     /locus_tag="OO309_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR5849174.1"
                     /translation="NAGLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTR
                     ITLRLPWRDEA"
BASE COUNT          251 a          272 c          209 g          258 t
ORIGIN      
        1 gtcattatta ttggctttca aaatgtagcg cacgctgcgc agaatatcgt tacttatcat
       61 gaggccttca ggtgttgatg aggcaaaaag ccattttagc agtcttttac aggccaatcg
      121 cctcttttaa gcttttcaga taacggcggc tgaccggcac ggttaagcca ttacgcaaaa
      181 tcaactcggc ctggccgtta tcttccagac gaatctcctg taaatgcgcg aggttaacca
      241 gatactgacg atggcagcgc agtagtggtg tacgactttc caaggtacgt aatgtcaatt
      301 cggtaaagcc ctctttccct tcgtggctgg taacgtagac accgctcatc cgactgctga
      361 caaatgccac atctttcatt tgcagcaaat aaatccgact atgccccgta caagggataa
      421 atttcagcgc ctgttgattt tccggtaaca gcgaaacatc ctgcttgctg cgctcctgac
      481 gcaatcgcgc cagcgttttc tccagtcgcg cttcatcaat tggcttcagc agataatcaa
      541 aggcatgttc ttcaaaggct ttaatagcgt attcgtcaaa cgcagtgaga aaaacaatat
      601 acgggcgatg ttccgggtca agcatcccca ccatttccag accactgatg cgcggcatct
      661 ggatatcgag aaacagcaca tccgggcgca gtttatgcac cgcgccgatc ccttccacgg
      721 cgtttgaaca ctctccaacg atttcaatat cgctctgctc ctgcaaaaat atacgcaggt
      781 tctcccgtgc taacggttca tcatcgacaa ttaagacttt aatcatgcct cgtccctcca
      841 tggtagtcgt aacgttattc gggtgtaact atcaggctca caggcgacgc ttattccata
      901 gtcatcgcca aaccgttcac gtaaacgctt atccaccaga ttcatcccca gcccactggc
      961 attggttacc ggttgataca aaccggcatt
//
LOCUS       DAJTQQ010000280          984 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000280 DAJTQQ010000000
VERSION     DAJTQQ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.293"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..711
                     /locus_tag="OO309_004300"
     CDS             <1..711
                     /locus_tag="OO309_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR5849175.1"
                     /translation="KDAARMLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMVNKPAPSM
                     PVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITFTGICYSH
                     RVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISASGVHNHFGV
                     SWFNPEDLATKRKVMNRGLRKILLARHALFDEVASASLAPISAFDVLISDRPLPADYV
                     THCQNGSVKIITPDSEDE"
     gene            complement(713..963)
                     /gene="ilvN"
                     /locus_tag="OO309_004301"
                     /pseudo
     CDS             complement(713..963)
                     /gene="ilvN"
                     /locus_tag="OO309_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          257 a          241 c          216 g          270 t
ORIGIN      
        1 aaagacgcag cgcgaatgct ggaagtttct gtaatgacta ttcgtcgcga tctccatcag
       61 gaagatgaac ctctgccact gaccctactg ggtggctata ttgtaatggt gaataaaccc
      121 gcgccatcca tgccagtaat ccatgacgtt ccaaaaaatc atcgtgatga cttacctatt
      181 gcaattctgg ctgccggaat ggttaatgaa aatgatctga tcttctttga taatggccag
      241 gagataccac tcgttataag catgatcccg gatgcaatca ccttcaccgg catctgttac
      301 tcacatcgcg tctttgttgc gttgaatgaa aagcctaatg taacagcaat actttgtggt
      361 ggtacgtatc gtgccagaag tgatgctttt tacgatgcca gtaactcttc gccattagac
      421 tctctcaatc cgcgaaaaat atttatttcc gccagcggtg tgcataatca ctttggcgtc
      481 agctggttta accctgaaga tcttgccact aagcgtaaag tgatgaaccg tggactacgg
      541 aaaattttgc tcgcccgcca cgcgttgttc gatgaagtgg cctctgccag cctcgcaccg
      601 atctctgcat ttgacgttct gattagcgat cgtccgttac cggcagatta tgttacgcac
      661 tgccagaatg gttctgtaaa gatcattaca cctgattcag aagacgaatg acttactgaa
      721 aaaacaccac aatcttgtta aacatcgtcg ggttggactg attacgttgc actttcacca
      781 catattccag cttatctatt tggcttatca cctactccag acgctggtca tccttgacca
      841 gtagccagat atggcttttg tcgcagtcct gaatcggcag gcagagaatg tcttcaacgt
      901 taagagcgcg acgggcaaaa gcccacaaag gtgagtcatt acacccggat ggtaaacagg
      961 cacttccttg ttgtagaagc cgtt
//
LOCUS       DAJTQQ010000281          980 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000281 DAJTQQ010000000
VERSION     DAJTQQ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.294"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..>980)
                     /gene="treF"
                     /locus_tag="OO309_004302"
     CDS             complement(<1..>980)
                     /gene="treF"
                     /locus_tag="OO309_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HCR5849176.1"
                     /translation="YLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASA"
BASE COUNT          231 a          260 c          274 g          215 t
ORIGIN      
        1 gggcactggc cttctggcgg aacagcgctt ctgtctcttt ctcgcctttc agcgccgaga
       61 tgttggcgat ggcgctctcc agtttaaaca ggaaggcatt cagatcgatg gggatgaact
      121 gggtggtacg aatgctcgcc agacgaccag tatcacgcag ccaacgggaa gagtaatccc
      181 aaccggaggc cgctcccgcg cgtaaatcgc ggtacacctc gttgggcggg cgaccagaat
      241 gtttcgcggt ttcaacgtcc tcaagccagg attcgtcacg cggcgtgtcg cgatcgtccc
      301 agtaacggtt gagcagcgat ccgtccggca tccgcacaac atggcgatag gcctgattag
      361 ggatcaacga ttctgcaccg tccatccaga aggcatattc cattttaagg tggtcgagat
      421 agcggcgcgc accgcgtaca ccatcttctt caaacaactc caccatcagc gcaaaaaccg
      481 gtggttgcga gcggctcaaa taataggtgc ggttgccgtt ggggatgtga ccgtagtttt
      541 cgatcatcca ggcgaagtta tcggccatgc atttcagcaa atcttcccga ccactttccg
      601 ccagtcccag catggtgaaa taggaatccc aatagtaggt ttcgctaaaa cggccgcccg
      661 ggacaatata tgactgcggt agcgccagca gagaagacca cggaatgtga tcctgtggtt
      721 cgcgggttag caccggccac agctggtcga tatgctcttt cagggaattt tgcgggtccg
      781 atacatactc gctggagtag acctccggca gccagaagtg attttcaaca aacttgcgca
      841 agtcaaaatc acgatgacgg cgcactttac ggtagcggat taaaatatcc agcgggtcca
      901 ttttgggtgc gcagtcggga aaggttttac tgtcggggaa aattttcgcc gactgaacat
      961 gctcgaacag ttcgagatag
//
LOCUS       DAJTQQ010000282          974 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000282 DAJTQQ010000000
VERSION     DAJTQQ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.295"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            418..>974
                     /locus_tag="OO309_004303"
     CDS             418..>974
                     /locus_tag="OO309_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5849177.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDS"
BASE COUNT          348 a          188 c          157 g          281 t
ORIGIN      
        1 acaaagccaa cggtattctg atttctgaag ttaatttcag actcgtttaa gctccatttt
       61 caaaaaaaat atatcagggt ttgttactaa taacttctga aaacagaaaa aaatagagca
      121 gtaattctat ttcagagagg agtaactcca tctatcctcg atacgtactg aacgttatcg
      181 tgatatattc atcatgcttt cttcaccatt attcgagtat agggagagac gcaatgacct
      241 taaatacctt tttatggctg aattaccatt ctcaggaata acagcagact ccttcccata
      301 gacggcttag ggggaaagca atacccggag aaagggtaca aagcaaactg ttttaaaatt
      361 aataagccat attctattta actagaattc ttatcgttga taagaattct agttataatg
      421 attaaatcaa ccaatataca ggcaatcggt tctggcatta tgcatcaaat aaacaatgta
      481 tactcgttaa ctccattatc tttacctatg gaactgactc catcttgtaa tgaattttat
      541 ttaaaaacct ggagcgaatg ggaaaagaac ggtaccccag gcgagcaacg caatatcgcc
      601 ttcaataggc tgaaaatatg tttacaaaat caagaggcag aattaaattt atctgagtta
      661 gatttaaaaa cattaccaga tttaccgcct cagataacaa cactggaaat aagaaaaaac
      721 ctattaacac atctccctga tttaccacca atgcttaagg taatacatgc tcaatttaat
      781 caactggaaa gcttacctgc cttacccgag acgttagaag agcttaatgc gggtgataac
      841 aagataaaag aattaccatt tcttcctgaa aatctaactc atttacgggt tcataataac
      901 cgattgcata ttctgccact attgccaccg gaactaaaat tactggtagt ttctggaaac
      961 agattagaca gcat
//
LOCUS       DAJTQQ010000283          967 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000283 DAJTQQ010000000
VERSION     DAJTQQ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.296"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(3..485)
                     /locus_tag="OO309_004304"
     CDS             complement(3..485)
                     /locus_tag="OO309_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5849178.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(476..778)
                     /locus_tag="OO309_004305"
     CDS             complement(476..778)
                     /locus_tag="OO309_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5849179.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
BASE COUNT          294 a          236 c          195 g          242 t
ORIGIN      
        1 actcagactt tataaaacaa ggtattaggt gccacaactg actgaacaaa accaaaacgc
       61 tcataaaatg ctctggcgtc atcatcaatt gcatgtacca taacagcctt aatgccaacc
      121 tgatcagcca gattgacaac ccggtgaatg gcatcactta gtagccattt accgaaacca
      181 tgcccctggg tacaaacatc aacagccaga cgccctaaca gaacaacagg gacaggattg
      241 ggcatgttat gccgcaaact gcgaccaagc tcggcatgag tgacggaacc agtagctaat
      301 gagtagtatc ccacaatacg taaagtatcc tccaagcaaa taacgaacgt gcgagaagca
      361 ttgagcatct ggtttttcat agcccgacca cgtaaccagt tacttagcac ctcatttcca
      421 cagtcaaatg gctgaagtat atgctcatcc gtcaacaatg ctggcgcggt gacatttatt
      481 tccattcagg ctttaccgcc atcagacgtt cacgcccttc tgcattctgc acaggagctt
      541 ccagttgcgt aataaacgaa tcataacgtt cgttatccag cacaaataaa cgttgatcca
      601 ggataatgtt tttagcttcc tggtacgcca gttccatgat gaaatcggta cggttcttat
      661 tgaccaagct cgcagcataa tcaataacgg ctcgttcctc atcagttgcc cggatgttaa
      721 tctgatgttc tcgcggtgtt ttacggacgc ttgcagctgt agacataaaa acctccattg
      781 gtaacaattt gctatgcaat taatgttatc gcaaatggta acagaacgct atacagaatc
      841 acttacggga aaagcgatcc cctgataaaa acaatggcag agacctgaac attgtgacga
      901 tgatctgtgt cggcgcacaa atcatgtctg attacggccg tttaatgtga ttttgataat
      961 gaaatgc
//
LOCUS       DAJTQQ010000284          956 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000284 DAJTQQ010000000
VERSION     DAJTQQ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.297"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(431..679)
                     /locus_tag="OO309_004306"
     CDS             complement(431..679)
                     /locus_tag="OO309_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5849180.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          240 a          181 c          223 g          312 t
ORIGIN      
        1 acactatcct aaactggatc ggtttaatgt aaaaaaacga tactttttgg ggggggggca
       61 atttttcctt aaggtatgaa aaaaggagct gaagctatat aatagctatt aatgccactg
      121 gtatcagtga atcatgtata taaaaaactg tgttaaggag gctgtccatt agcggttttt
      181 tattcttctg gaaccgcggc atctttgcgt atctttttgt cttctcaccg tctggtcggt
      241 gttttgctga gactgctaat ttcctgtctt tgctagtgct gtactggtac tgcccaatca
      301 tgattggcgg gggcgggcgg agttgagacc gcagacacgt cgtatgcaag aacgtgctgc
      361 ggctggctgg tgaacttccg atagtgcgag tattgaatga tttccagccg ttactgattt
      421 tacgtgctaa ttagtgaaca aaccactcgt cagcagactc ccaggtatct ttcagagttt
      481 cctgaacaaa agttttagct gaatctttat cggcggtgcg cgtaacagaa aggccatcgt
      541 tgctggtggc ttttacgatc acctctacat cgtcataacg tttactgatg cgtcgggtta
      601 attcttcctt taacgcatcc acagcaccgg ttggcatttt agtcattttc tctttggcta
      661 tgcagatttc aaaacgcata aaaatccctc tgcactgtgt ttttatacag tattattttt
      721 aactgtatgg ataaacagtg tcaagagtga gggaaagtga ttttttgggg catgcatggg
      781 gcaaaaatat ttgttgtggg gcattttggg gcatgattgg gacactttgt tacatatgaa
      841 ctttgccgat tttcatatga catggcaatc caacgctttg attattctaa agaaaacaca
      901 tgctcttggg cgttctttag tgatttttaa aaatcccgta tcacgcagtt aaagtg
//
LOCUS       DAJTQQ010000285          953 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000285 DAJTQQ010000000
VERSION     DAJTQQ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.298"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..>953
                     /locus_tag="OO309_004307"
     CDS             <1..>953
                     /locus_tag="OO309_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5849181.1"
                     /translation="PNLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIHGV
                     WFDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMTIY
                     DAEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHFYV
                     IGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTAWP
                     HDGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFKVF
                     NTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFA"
BASE COUNT          246 a          232 c          264 g          211 t
ORIGIN      
        1 ccgaaccttg ttgatcacct tctggttaag catcacacgg ctgatggcgt tgaagatggc
       61 atttcaatct gctacttcaa gccatactcg caaggccgtg ctcgctggca gggtgacaca
      121 atccacggcg tgtggtttga cgaagagcca ccatacagca tttatggcga aggtcttacc
      181 cgtaccaaca aatacgggca attctcaatt ctgacgttta ccccgctgat ggggatgtct
      241 gacgttgtta ccaagttcct gaagaacccc agcaagtcgc agaaagtggt caacatgacc
      301 atctatgacg ctgagcacta taccgacgag cagaaagagc aaatcatcgc atcatatccc
      361 gagcatgaga gagaggcgcg tgctcgcggt attcctacga tgggtagtgg tcgaatcttc
      421 cagataccgg aagagacgat taagtgtcag ccgttcgagt gtcctgatca cttctacgta
      481 attggcggga tggatttcgg atgggatcac ccacaggcgc aagttcagct ttggtgggat
      541 aaggaggcag acacaatcta cgtttcacgc gtgtggaagg cgaaagaaaa aacagccgtt
      601 caggcgtggg gagccgttaa atcatgggcg cataaagtgc caaccgcatg gcctcatgat
      661 ggaaaccagc acgagaaggg cggcggtgag cagctcaaag ggcagtatgc agacgctgga
      721 tttatgatgt tgcaggagca tgcgacatgg cctgatggcg gtaacgctgt tgagccaggc
      781 atcactgaat tgcgcgacat gatgctcgat ggtcgcttca aagtattcaa tacctgtgag
      841 ccattctttg aggagttccg cctctatcac cgtgatgaaa acgggaaaat cgtcaagctt
      901 aacgacgacg tgctatccgc cgttcgctat gcatacatga tgcgccgctt cgc
//
LOCUS       DAJTQQ010000286          952 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000286 DAJTQQ010000000
VERSION     DAJTQQ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.299"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..269
                     /locus_tag="OO309_004308"
     CDS             <1..269
                     /locus_tag="OO309_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5849182.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            266..616
                     /gene="tnpB"
                     /locus_tag="OO309_004309"
     CDS             266..616
                     /gene="tnpB"
                     /locus_tag="OO309_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5849183.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFTKRLAGDPGRESAPDASSVIHATGGDRVATSQTDR
                     TAWHPDITRDKTRE"
     gene            613..>952
                     /locus_tag="OO309_004310"
     CDS             613..>952
                     /locus_tag="OO309_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5849184.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSENPIASKPE"
BASE COUNT          259 a          244 c          263 g          186 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacagata tgcgttgtgg cttcaatggc ctggcttcga aggtgcagaa
      361 cacgctgaaa gatgacccgt tctccgggca tatcttcgtc ttccggggcc gcagtggcaa
      421 aatggtgaaa atactgtggg ccgatcgtga cgggttatgc ctgttcacca aacgtctggc
      481 cggtgacccg ggaagggaaa gtgcacctga cgccagctca gttatccatg ctactggagg
      541 ggatcgcgtg gcaacatccc aaacggacag aacggcctgg catccggata taacccgtga
      601 taaaacaagg gaatgaacaa tgaactcccc gatgatattg agctgcttaa agccatgttg
      661 cgtaagcaac agagtcggct tcgacagtat gcctgtcagg tcgcgggcta tgagcaggaa
      721 attgaacggc tgaaagcgca actcgacagg ttgcgtcgta tgttgttcgg ccagagttca
      781 gagaaaaagc gtcataagct tgaaaatcag atccgacagg cagaaaaacg actgtcggaa
      841 ctggaaaacc ggctgaacac agccagaaat cttctggaag atgcatcgtc agtcacagat
      901 tcacctgaca ccagtccccc gtcagaaaac ccgatcgcca gtaagcctga at
//
LOCUS       DAJTQQ010000287          948 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000287 DAJTQQ010000000
VERSION     DAJTQQ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.300"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..289
                     /locus_tag="OO309_004311"
     CDS             <1..289
                     /locus_tag="OO309_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5849185.1"
                     /translation="RRAEIVAGSEHIVPADAVIMAFGFRPHNMEWLAKHSVELDSQGR
                     IIAPEGNDNAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGIMNWLEV"
     gene            473..877
                     /locus_tag="OO309_004312"
                     /pseudo
     CDS             473..877
                     /locus_tag="OO309_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
BASE COUNT          244 a          235 c          236 g          233 t
ORIGIN      
        1 tcgccgcgcg gagatcgttg caggttccga acatatcgtt ccggcagatg cggtgatcat
       61 ggcgtttggt ttccgtccac acaacatgga atggctggca aaacacagcg tcgagctgga
      121 ttcacaaggc cgcatcatcg ctccggaagg caacgacaac gctttccaga ccagcaaccc
      181 gaaaatcttt gctggcggcg atatcgtccg tggttccgat ctggtcgtta ccgccatcgc
      241 cgaaggtcgc aaagcggcag acggcattat gaactggctg gaagtttaag ctctgtccca
      301 gcgccgcctc ctcagaaaag agtcggcgct gaaattctcc tttctttctg gaaatcgcct
      361 cgcataatct gaagaaaacc aatgtaatac gctcatctgg attgttaccg gtagatgccc
      421 ggcgtatagc tgattccatt attcaacgca gcgccatata aagggacaga taatggaatc
      481 gctctcagag agaacctcaa caggctacca gcaaatccac aacggcatta ttcatctggt
      541 cgatagcgcc cgaacggaaa cggtacgtag cgttaacgcg ctgatgaccg cgacgtactg
      601 ggaaattggc cgacggattg tcgaatttga acaaggtggt gaggccagag cagcttatgg
      661 tgcacagttg attgatcggc tatcgaagga tttaagtcga cggtataaac gtggggtctc
      721 tgcaaaaaac ttacgccaaa tgaggctttt ttacctcttt tttcaacatg ttgaaattcg
      781 ccagacagtg tctggcgaat taacaccatt gggaattccg cagacaccat ctgcagaatt
      841 cccatcggca aagatttggc agacgctgtc tgccaaattg agaggctgtt tgatatttcc
      901 acagcttttg tattgcatct cctattttcg gtttgtatat tcatgcaa
//
LOCUS       DAJTQQ010000288          947 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000288 DAJTQQ010000000
VERSION     DAJTQQ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.301"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(201..866)
                     /locus_tag="OO309_004313"
     CDS             complement(201..866)
                     /locus_tag="OO309_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR5849186.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          245 a          232 c          215 g          255 t
ORIGIN      
        1 gtaagaggct aacctacatg tgcaaagcat gaaattggcc tcagattaat gttaagcgtc
       61 ttgccggacg cgtaatgtta actggggctt ttctctatct gccttttggt gttcatgcct
      121 gaagcagata gcctcaagca cccgcagtca ttctacttac ctcgccaata tgaaatcaat
      181 cagaaaggtg ccccataaaa tcactccttc tcttctttac cgtagtggag ttgaccaatt
      241 ttgataagag ggcgtccctg agatttgcgg tgtagattgg tatcgcgcag agaatacaca
      301 cagccacaat attcctgctg atagaatttt tcgcgcttgc tgatttcaat catacgggac
      361 gagccgccct gcttgcgcca gttataatcc cagtacacca tgcccggata atgcgcgacg
      421 gctcgccgcc cacagtcgtt aacctgctgc atatttttcc agcgtgaaat gcccagtgaa
      481 ctgctgatca cactgaaacc attttcagcg gcgtacagcg ctgtccgctc aaaacgcatg
      541 tcaaaacaca tggtgcaacg gatccctcgt tcgggctccc attccattcc tttggctcgt
      601 tcaaaccagt tgtctgtgtc gtaatcagca tcaataaacg gcacgccgtg ttgttcagca
      661 aagcgaatat tctcatcctt acgaattaaa tactctttct gaggatgaat gttcgggttg
      721 tagaaaaaga tggtgtagtc gatttccgag gcctgaagcg cctccatcac ctcaccggaa
      781 catggagcac agcaagagtg cagtagtagt ttgtttgccc cgtttgggag ctccaatttg
      841 ggccgtttga aatcagcaac tgtcataaat atgtttattg aggtcataaa aatatcaaag
      901 agtgtagcat tagaacgggg ctatcggaaa cagatgtgtg actgctc
//
LOCUS       DAJTQQ010000289          936 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000289 DAJTQQ010000000
VERSION     DAJTQQ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.302"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(1..801)
                     /locus_tag="OO309_004314"
                     /pseudo
     CDS             complement(1..801)
                     /locus_tag="OO309_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
BASE COUNT          235 a          255 c          219 g          227 t
ORIGIN      
        1 tgttgaggcc aatggcagcg ggaagtgcag ggctttggct gtatcggcaa ccagaccaag
       61 atccttaaca aaaatatcga cggctgaatg cggggtgtaa tcgccatcca ccacatgacg
      121 catccggttt tcgaacatcc aggaatttcc ggcggcattg gtcacgacgt catacatcac
      181 atccagcggg atccccgcac gggctgcaag tgccatcgct tcggctccgg cagcaatatg
      241 tacgcccgct aacaactggt gaataatttt tacggtcgaa cctagtcccg gttctgcacc
      301 tatgcgataa acttttccgg caacggcttc cagcacgggt accagtcgtt caaaggcaat
      361 atcgctaccg gaggccatga cagtcatttc accgttagcg gcttttactg caccaccaga
      421 aactggcgca tccagcattt ccagatcgaa tccagccaga gcggtagcaa tttcttgcgc
      481 atcagcacta gcgatagtgg aagaaaccat tactgccgta ccgggtttca gatgttgtgc
      541 aacgcctgtt tcaccaaaca gcacctgttt aacctaggcc gcattgacca ccagcaccag
      601 cagagcgtcc agtttttcgg caaacgtcgc ggcgttatca gaaaccccgc aagcacctgc
      661 ctctttcaac gtagcgcagg cattgctgtt caggtctgcg ccccaggtag aaaaacctgc
      721 gcggacacat gacagtgctg ctcccattcc cattgaccct aagccaacga taccgacatg
      781 aaactcagat cccgttttca tctgctctcc ttgttaattt aagtgatatt ttgtttgata
      841 ttgtgaacat aagcgctggg agataacgat atggtgagat gattcacata aattaacatt
      901 ttgtgttatt ttatgtgaac taggcgttag ttgcgc
//
LOCUS       DAJTQQ010000290          932 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000290 DAJTQQ010000000
VERSION     DAJTQQ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.303"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            149..>932
                     /locus_tag="OO309_004315"
     CDS             149..>932
                     /locus_tag="OO309_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5849187.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQS"
BASE COUNT          316 a          225 c          132 g          259 t
ORIGIN      
        1 ataacatatg gagttaatat gcttacacaa actatatatc cgcctcctcc agaagttcct
       61 gtctactgct gaagggaacc aataacattg caaagcagat aaaaaagaat ccaacacctc
      121 tcaacataaa gaatacaccc tgatatttat gctcccgaca aataacaatc acagattaat
      181 ttcaaattcg ttctccactt attcaatcga cactagccgc gcatatgaaa attatctaac
      241 ccattggact gaatggaaaa ataaccgcat acaagaagaa caacgagaca tcgcttttca
      301 gcgactagta tcatgtctac aaaaccaaga gacgaacctg gacttgtctg aattaggcct
      361 gacaacatta cctgaaatcc ccccgggaat taaatcaatt aatataagta aaaataattt
      421 aagcttaatc tccccattgc ctgcgtccct tacacaactt aatgtcagct ataacagact
      481 tattgaactg cctgctttgc ctcaaggact taaattattg aatgcgtccc acaatcaact
      541 aatcacacta cccacactcc ccatatcttt gaaggagctt catgtctcaa ataatcaatt
      601 atgttctctt cctgttttac cagaactact ggaaacatta gatgtatcat gtaatgggct
      661 ggcagtttta ccacctttac cattttcttt acaagagatt agcgcaatag ggaatcttct
      721 tagtgaactc ccccctctac ctcacaacat tcactccata tgggcaatcg acaatatgtt
      781 aaccgatatt ccatacctgc cggaaaattt aaggaacggt tattttgaca taaatcagat
      841 aagtcatatc ccggaaagca ttcttaatct gaggaatgaa tgttcaatag atattagtga
      901 taacccattg tcatcccatg ctctgcaatc cc
//
LOCUS       DAJTQQ010000291          925 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000291 DAJTQQ010000000
VERSION     DAJTQQ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.304"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..699
                     /locus_tag="OO309_004316"
     CDS             <1..699
                     /locus_tag="OO309_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HCR5849188.1"
                     /translation="QRELEQQMEAVKRGELPEHLRVNPELPKQPDPNDYLSEDALAKY
                     DYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSAQYVEAARKHYDAA
                     EKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGANPEKTRQLLAMDGQ
                     SALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIEKQMEAAANKGDVE
                     TYRKLKAQLNKGIR"
     gene            699..>925
                     /locus_tag="OO309_004317"
     CDS             699..>925
                     /locus_tag="OO309_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCR5849189.1"
                     /translation="MALNEGQLVTYALDEIIETVQNLTPMASKVTKYTPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSV"
BASE COUNT          264 a          249 c          242 g          170 t
ORIGIN      
        1 cagcgtgagc ttgagcaaca gatggaagcg gttaagcgtg gagagttgcc ggagcacctg
       61 cgggtgaacc ctgagttacc aaaacaacca gaccctaacg attatctttc cgaagacgca
      121 ctggctaagt acgactatga ccagagccgc gcactggctg ccttccagca ggcaaacagt
      181 gaatggcaga tcaaggctat ggacgcacga agccaggctg tagccgagca gggccgcaaa
      241 actcaggagt tcacccagca atcagcgcaa tacgtcgagg cagcccgtaa gcactacgac
      301 gcagcggaaa agctcaatat ccctgactat caggagaaag aggatgcatt catgcaactg
      361 gtgccgccag cagtcggtgc cgacatcatg cgcctcttcc cggagaaatc cgctgctctc
      421 atgtatcacc ttggtgctaa tcctgagaaa acacgccagt tgctggcgat ggacgggcaa
      481 tccgcgctga ttgaactcac tcgactgtca gaacgtttaa ctctcaagcc tcgagccaag
      541 cctgtttcag aagccccgtt acctgatgaa cctattcagg gtcacgctgt tgctgcaaat
      601 atctctgcga ttgaaaagca gatggaagcg gcagcaaaca aaggggatgt agagacgtac
      661 cgcaagctca aggcgcaact gaataaagga attcgataat ggcattaaat gaaggtcaac
      721 tggtcacgta tgctctggat gaaatcatcg aaaccgtcca gaacctgacg ccaatggcgt
      781 ccaaagtgac aaaatacacc cctccggcag aatccatgca acgttcaagc aacaccgtgt
      841 ggatgcctgt tgagcaggaa gcgccaaccc agactggctg ggatttaact ggcaacgcaa
      901 ccgggattct ggaactctcc gtgaa
//
LOCUS       DAJTQQ010000292          915 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000292 DAJTQQ010000000
VERSION     DAJTQQ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.305"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..496
                     /locus_tag="OO309_004318"
     CDS             <1..496
                     /locus_tag="OO309_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5849190.1"
                     /translation="LHASTNAPGSDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQK
                     THYSDTRWHEDSVRNRWFSVMAGLSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDN
                     KGKTHDVLTGSDDNRHSNTSLAWGAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGV
                     GYKF"
     gene            518..>915
                     /locus_tag="OO309_004319"
     CDS             518..>915
                     /locus_tag="OO309_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5849191.1"
                     /translation="MTARWFRRAFCGVNMAVKISGVLKDGAGKPVVNCAIELRARRTS
                     PTVVAHVVATCVTDNNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNA
                     FLNAPKDGDLRPEVMKRFEEMVAQAQQSAGA"
BASE COUNT          199 a          207 c          300 g          209 t
ORIGIN      
        1 tcttcatgcc tcgacgaacg ctcccggcag tgatgatctg aacgggatta acgtgaaata
       61 ccgttatgag tttacggaca cgctggggct ggtgacgtca ttcagctatg ccaacgctga
      121 agatgagcaa aaaacgcatt acagtgatac ccgctggcat gaggattccg tgcgtaaccg
      181 ctggttcagc gtgatggcgg ggctgtctgt gcgcgtgaat gaatggttca gcgcgtatgc
      241 gatggcgggc gtggcttaca gccgtgtgtc gaccttctcc ggggattatc tccgcgtaac
      301 tgacaacaag gggaaaacgc acgatgtgct gaccgggagt gatgacaacc gccacagcaa
      361 cacgtctctg gcgtgggggg ctggcgtgca gtttaacccg accgaatccg tggccattga
      421 ccttgcttat gaaggttccg gcagtggcga ctggcgcact gacggtttca tcgtgggtgt
      481 cggttataag ttctgattag ccaggtaaca cagtgttatg acagcccgct ggttcaggcg
      541 ggctttttgt ggggtgaata tggcagtaaa gatttcaggt gtactgaaag atggggcggg
      601 taaacctgtc gtaaattgtg cgattgaact gcgggccaga agaaccagtc cgaccgttgt
      661 ggcacacgtt gttgccactt gcgtgacgga caataacggt gcttatgtga ttgaggctga
      721 gccggggtat tacgaggttg cgcttcactg taacggctgg cagccaaccc gtgtcgggga
      781 tattgatgtg gcaccgactg atgcaccggg gacactgaac gcgtttctga atgcaccaaa
      841 ggatggtgat ttacgtccgg aggtgatgaa gcgctttgag gaaatggtgg cgcaggcgca
      901 gcagagtgcc ggggc
//
LOCUS       DAJTQQ010000293          895 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000293 DAJTQQ010000000
VERSION     DAJTQQ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.306"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..160)
                     /gene="ospE1"
                     /locus_tag="OO309_004320"
     CDS             complement(<1..160)
                     /gene="ospE1"
                     /locus_tag="OO309_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HCR5849192.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAA"
     gene            complement(269..399)
                     /locus_tag="OO309_004321"
                     /pseudo
     CDS             complement(269..399)
                     /locus_tag="OO309_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612303.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(397..492)
                     /locus_tag="OO309_004322"
                     /pseudo
     CDS             complement(397..492)
                     /locus_tag="OO309_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          260 a          152 c          206 g          277 t
ORIGIN      
        1 tggcagcagg tatttgtaat tcgtatatag ctgctttttt attgaaaaca gtatatccat
       61 ctgtagtaac agtagaagat aataatacag gatttgatac ttcaaggatg atattttctt
      121 gtttttgtgg aagacaaggg aatatagttt gtgtaagcat attacttcct tatgttatat
      181 tttggtaaaa aatttatttt tttacattat agtcggctaa atgaagttta ctgtaaaaaa
      241 acgatacttt ttcgggtggc gcttccaatc agtagaatat gtgtttttca atgaaggagc
      301 cgatgacttt tcgtggattt ccacggaacg cgagaagcag attgttttac gtgccagccg
      361 cttgatgcgg gtgcggaggg tcaggttgtt acgttcaaga tatagcatgt gctggatgaa
      421 gatccctttt ctggtcacct gtttatcttt cgtggccgac ggggtgacac tgttaaaatg
      481 taatgaacgc atcccacaca attaaacacg gagtagtatg atggttaaaa gggtgaaact
      541 ggcaggtgtg gagcctcgca gctttccggc atcatactgc ccaatacggg atgcataaag
      601 cgtcctcaga ctggagactc cacaaatgaa atatacaccg gttggcgttg atatggcaaa
      661 acatgtcatt cagattcact tcatcaatga gcacatgtca gcgccagtga tataagacgg
      721 taatggggat gcggacgatt tcctggactc tcagaagatg aaacatgttt actgaagaac
      781 aaaaaattcg cgctattgag ctttatttca aatatggtaa gaagcttgag gtgtactggc
      841 aatagcggac accaccattt gttctttttc taagcagtca tctgataata tttta
//
LOCUS       DAJTQQ010000294          890 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000294 DAJTQQ010000000
VERSION     DAJTQQ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.307"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..156
                     /locus_tag="OO309_004323"
     CDS             <1..156
                     /locus_tag="OO309_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 family protein"
                     /protein_id="HCR5849193.1"
                     /translation="HDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEI
                     WRKEGYL"
     gene            153..707
                     /locus_tag="OO309_004324"
     CDS             153..707
                     /locus_tag="OO309_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR5849194.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
BASE COUNT          234 a          186 c          258 g          212 t
ORIGIN      
        1 cacgatatgc aggcagcgat tgcctgtagc tcatgccacg atttaatcga cgggcgggta
       61 aaaaccagcg attacaccaa agaagaatta cgcctgatgc atgcagaagg tgtttttcgc
      121 acacaagaaa tctggagaaa ggaaggttat ttatgattta cccaacaaat acaggcaaaa
      181 gcggggaaca ccttcgtctc accacgctgg aaagtgtctg gattcaggga aaactgcgca
      241 tgtgggggcg ctggtcgtat attggcggcg gtaagacggg aaatatgttt aaccagttgc
      301 tggcatccaa aaaattgacg aaaacagccg tcaatgaagc cctgcgcagg atgaaaaaag
      361 cgggaataga gaaacctgag ctggaagcgt ttttgcgaga gatgatcaat ggcaagcaaa
      421 agacctggct ggcgcattgt actgatgcag aggcgttatg tattgataga gtcataagtg
      481 aggtgctggc agagcatcct ggattgatta gtgtcctccg gcaacggtat gaagggcggg
      541 ggatgactaa gcgtaaaatg gctgaattgc tgaatgacgc acaccctgag tggtgtttcc
      601 ggacgtgctg cagtcgggta gatgtatggc taaatcttgc tgaatatatg ctctatctac
      661 cgatgcgtga tgcattctct tccggggatc taaaaaccgt ttgttgactc aatctgttat
      721 ccggggctat attcctcacg cgccagcaaa atctggcgtc gggattggcg tcccggatgt
      781 ttacggagcg atatgagacg cgcccgcgtc ttttttcata tcgtttgcac agtcacattc
      841 gcgatttatg gcgggctgtg tgggggagcc gaaaggctcg ccggtttccg
//
LOCUS       DAJTQQ010000295          877 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000295 DAJTQQ010000000
VERSION     DAJTQQ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.308"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            2..>877
                     /gene="narH"
                     /locus_tag="OO309_004325"
     CDS             2..>877
                     /gene="narH"
                     /locus_tag="OO309_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5849195.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT          231 a          226 c          243 g          177 t
ORIGIN      
        1 aatgaaaata cgttcgcaag tcggcatggt gcttaacctc gataaatgta tcggctgcca
       61 tacctgttcg gtgacctgta acaacgtctg gaccggacgc gaaggcatgg agtacgcatg
      121 gtttaacaac gtcgaaacca aaccgggcat tggttatccg aaaaactggg aagatcagga
      181 agagtggcaa ggcggctggg tccgcgatgt gaatggcaag atccgtccgc gtctgggcag
      241 caagatgggc gtaataacca aaatcttcgc caacccggtg gtgccgcagc agattgatga
      301 ttactacgaa cctttcacct tcgactacga acatttgcat agcacaccgg aaggcaaaca
      361 tatccctact gctcgcccgc gttcactgat tgacggcaaa cggatggaca aagtgatctg
      421 gggcccaaac tgggaagaac tgctgggcgg cgagtttgaa aaacgtgccc gcgaccgcaa
      481 cttcgaggcc atgcaaaagg agatgtacgg gcagtttgaa aacaccttca tgatgtacct
      541 gccgcgcctg tgcgaacact gcctcaatcc cagctgcgtg gcgacctgcc caagcggcgc
      601 tatctacaaa cgcgaagaag acggcattgt gctgattgat caggataaat gccgtggctg
      661 gcgtttgtgc attagcggtt gtccgtacaa aaaaatctac ttcaactgga aaagcggcaa
      721 gtcagaaaaa tgtatcttct gttatccgcg aattgagtca ggccaaccca ccgtgtgctc
      781 agaaacctgc gtgggtcgca tccggtatct gggcgtgctg ctttacgacg ccgaccgcat
      841 cgaggaagcg tcgagcaccg agcgcgaagt tgacctc
//
LOCUS       DAJTQQ010000296          869 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000296 DAJTQQ010000000
VERSION     DAJTQQ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.309"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            46..>869
                     /gene="ldtD"
                     /locus_tag="OO309_004326"
     CDS             46..>869
                     /gene="ldtD"
                     /locus_tag="OO309_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5849196.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPG"
BASE COUNT          212 a          219 c          244 g          194 t
ORIGIN      
        1 taacagcctg gctattcaga gtatgataaa aacagggggc aagggatgtt gcttaatatg
       61 atgtgtggtc gtcggctgtc ggcaatcagt ttgtgcctgg ccgtaacatt cgctccactg
      121 ttcaatgcgc aggccgatga gcctgaagta atccctggcg acagcccggt ggctgtcagt
      181 gaacagggcg aggcactgcc gcaggcgcaa gccacggcaa taatggcggg gatccagcca
      241 ttgcctgaag gtgcggcaga aaaagcccgc acgcaaatcg aatctcaatt acccgcaggt
      301 tacaagccgg tttatcttaa ccagcttcaa ctgttgtatg ccgcacgcga tatgcaaccc
      361 atgtgggaaa accgtgatgc tgttaaagcc ttccagcaac agctggcaga ggtggcgatt
      421 gctggtttcc agccgcagtt taataaatgg gtagagttac tgaccgatcc tggtgttaac
      481 gggatggcac gtgacgtggt gctctctgat gcgatgatgg gctatctcca tttcattgca
      541 aatattccga tcaaaggcac tcgctggcta tatagcagta aaccttatgc gctttcaacg
      601 ccgccgcttt cggtgattaa ccaatggcag ctagcgctgg ataaaggtca attgcctacg
      661 tttgttgcag gactggcacc gcagcatccg caatatgcgg cgatgcatga atcgttactg
      721 gtcttactca gtgacaccaa accgtggccc caactgaccg gcaaagcaac gttgcgccca
      781 gggcagtgga gtaacgacgt accggcgttg cgcgaaatat tgcaacgcac aggcatgttg
      841 gacggggggc cgaaaattac tctacctgg
//
LOCUS       DAJTQQ010000297          840 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000297 DAJTQQ010000000
VERSION     DAJTQQ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.310"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..109
                     /locus_tag="OO309_004327"
     CDS             <1..109
                     /locus_tag="OO309_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR5849197.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
     gene            447..840
                     /locus_tag="OO309_004328"
                     /pseudo
     CDS             447..840
                     /locus_tag="OO309_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          252 a          177 c          204 g          207 t
ORIGIN      
        1 aaatagtggg gacactatgc ttgacaatgc aatgaaaagc aaagattcaa aaatgattga
       61 ttttttatta aaaaatggag caatattagg caaacgattt gagatatgac ctaaaagctc
      121 ggccaaataa taatgtcgtc gattatcgaa gtcatcatga gtgattctgt ccccacagtg
      181 cagcatcagc atgacagtga attgtctggc gtggtccatt taatgagatg gagatggtcc
      241 ctctttccta aatagctgtg cctaataccg ctacactttt gccagcccat gtttgcctcc
      301 ggggaatggg ctgacggttc cttcaaaatc cattgcacgc agttatgtcg gagcgggaac
      361 ttgactcctg tctttacggg agtgaggtag cctgagttta acggacactc cttcctgaaa
      421 tagaatggca tcagaaggag ccaataatga gcagaaaaac ccaacgttac tctacggagt
      481 ttaaagccga agccgtcaaa acggttcctg aaaatcagct ttcgatcagt gaaggagctt
      541 cccgattatc tgttcctgaa ggcactttag gacaatgggt taccgccgca agaaaagggc
      601 tcaatacacc aggctcccgc acagtggctg agctggaatc tgaagttatg caactgcgta
      661 aggctttaaa tgaagcacga cttgagcgag atatattaaa aaaagcaaca gcgtattttg
      721 cacaggagtc gctgaaaaat acgcgttagt cgaacaatgg cgacaacaat tccctgttga
      781 agcgatgtgt cgggtacctg gtgtatccag gagcggttat tacgaccggg tacagcatgc
//
LOCUS       DAJTQQ010000298          840 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000298 DAJTQQ010000000
VERSION     DAJTQQ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.311"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            1..840
                     /locus_tag="OO309_004329"
                     /pseudo
     CDS             1..840
                     /locus_tag="OO309_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          212 a          213 c          225 g          190 t
ORIGIN      
        1 cggtaagcgt tatcccgaag agtttaaaac tgaagcagtc aaacaggttg ttgatcgcgg
       61 ttattctgtt gccagcgttg caacacgtct cgatatcacc acccacagcc tttatgcctg
      121 gataaagaag tacggtccgg attcttccac taataaagaa cagtcagatg ctcaggccga
      181 gatccgccgt ctccagaaag agctgaaacg ggttaccgac gaacgggaca tattaaaaaa
      241 agccgcggcg tacttcgcaa agctgtccga ctgaggtacg cctttatccg tgacaactcc
      301 tgttgctggc ctgttcgcct gctctgtcgg gtgctggatg ttcatcccag tggtttttac
      361 gcctggcttc agcagccgca ttcacaacgc catcaggcag acctgagact gacaggacag
      421 attaaacagt tctggctgga atcgggatgc gtctatggtt atcgcaaaat ccatctggat
      481 ctgcgtgaca gcgggcaaca gtgcggagta aacagagtct ggagactgat gaaacgtgtc
      541 ggaataaagg ctcaggtcgg ataccgaagc ccgcgggcac gtaaaggcga ggccagtatc
      601 gtgtcaccca acaggctcca gcgacagttc aatccggatg ctcctgatga gcgttgggta
      661 acggacataa cctacatcag gacccacgaa ggctggctgt atcttgccgt tgttgttgat
      721 ctgttctcac gcaaaattat cggctggtcc atgcaatccc ggatgacaaa ggacattgtc
      781 ctgaacgcac tgctgatggc tgtatggcgg cgtaatcccg aaaaacaggt gctggttcat
//
LOCUS       DAJTQQ010000299          839 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000299 DAJTQQ010000000
VERSION     DAJTQQ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.312"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..575)
                     /gene="virK"
                     /locus_tag="OO309_004330"
     CDS             complement(<1..575)
                     /gene="virK"
                     /locus_tag="OO309_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR5849198.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCAT"
     gene            complement(580..>839)
                     /locus_tag="OO309_004331"
     CDS             complement(580..>839)
                     /locus_tag="OO309_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR5849199.1"
                     /translation="LAEASAFSVPVVATQIGGIPEVIQNNQTGTLLPASNKHAWMCAL
                     NDFFNDPGRFYQMARLAKQDIEERFDINKTALKILTLAKQK"
BASE COUNT          258 a          182 c          162 g          237 t
ORIGIN      
        1 gttgcgcacc gaataacatc aggtccggta tttttagggg ctccctgtaa tccgccaata
       61 aaagcaatgt gtccattttg agtcagaatg actgaaaatg ttaaccgggc aataactgtt
      121 ttattgtaaa acaatgacaa catcaactct ccttcccggt caaaaccact gggagaacac
      181 tggatatcaa aattctctcc atctttcccg atgaattgga ctaatgatac cgatttttgt
      241 ggaaacaaaa atttagaata tttttctggc aaattttgca caaagcgata atgctcaaga
      301 atatattgtc cacgggtcca ggcgcgcccc cctttatgca aataagggcg atgaattctt
      361 gctggcaacg tggggtgtat tgccagaata tcctcaatgc acggcaagga acgcagttca
      421 taataatatc gaatactgac aaccggattt atagaacatc gcaaaatgta catgaaccga
      481 aattttctgt gttcccactt ccccggtagt gaatctgagg aaaaaataat ccttttaagg
      541 aaaccgataa atgataagtt acttatagaa aacatattgt tacttttgct tcgctaatgt
      601 gagtattttt aacgcagttt tattaatatc aaaccgctct tctatatcct gttttgccag
      661 gcgagccatc tgataaaaac gcccagggtc attaaaaaaa tcattcaggg cgcacatcca
      721 tgcgtgctta ttacttgctg gtaacaatgt cccggtctgg ttgttctgaa taacctcagg
      781 gattccacca atctgagtgg ccactacagg cacagaaaat gccgatgctt ctgccagca
//
LOCUS       DAJTQQ010000300          829 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000300 DAJTQQ010000000
VERSION     DAJTQQ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.313"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..496
                     /locus_tag="OO309_004332"
     CDS             <1..496
                     /locus_tag="OO309_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5849200.1"
                     /translation="NIGSMFRNGWENNVKSIDALPHIEAGSNARTSDISSEEYAIMPL
                     APMKEADAPNEELRQAWEYYHTPRAQYPTAPGYATLRSLNQIITYDAYHMAEVYLTQP
                     MQIVAGSQAGSKWMSDDLYDRASSQDKRYHIVEGANHMDLYDGKAYVAEAISVLAPFF
                     EETL"
     gene            496..829
                     /locus_tag="OO309_004333"
                     /pseudo
     CDS             496..829
                     /locus_tag="OO309_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
BASE COUNT          226 a          188 c          210 g          205 t
ORIGIN      
        1 caatattggt tcgatgttcc gcaatggttg ggaaaacaat gtgaaatcta ttgatgctct
       61 gccacacatt gaagctggct caaacgcccg aaccagcgat attagcagcg aagaatacgc
      121 cattatgcca ctggctccaa tgaaggaggc cgatgcgccg aacgaagaac tacgtcaggc
      181 ctgggagtat taccacacac ctcgtgcgca gtatccaaca gcaccgggtt acgctacttt
      241 gcgcagcctt aaccagatta ttacctatga tgcttaccat atggcggaag tgtacctgac
      301 tcagccgatg cagattgtgg cgggcagcca ggcagggagc aaatggatga gtgacgacct
      361 gtacgatcgg gcatcaagcc aggataaacg ctatcacatt gttgaaggcg caaaccatat
      421 ggatctttat gacggcaaag cctatgtggc tgaagcaatt tcagtattag ctccgttctt
      481 tgaggaaact ctgtaatgaa acatattcaa atccgcaata gtgatatggc ctggcatatt
      541 gctgccaata ttcagtttcc accaaatttt gatgagtcaa aacagtatcc ggcgattatc
      601 agcgtccatc cttttggcag ttgtaaggag cagacgtctg gcaatatcta tggcaaggca
      661 ttggctgaaa aaggctatgt cgttctcgct tatgacgcca gtttccaggg agaatctggt
      721 gggtagcctc gttggataga agatccgaca cagcgcgtag aggatattag ccgtgttatc
      781 gattatgcag taactctgcc atatgttgat gctgaacgca ttggcgtgc
//
LOCUS       DAJTQQ010000301          778 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000301 DAJTQQ010000000
VERSION     DAJTQQ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.314"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            168..665
                     /locus_tag="OO309_004334"
                     /pseudo
     CDS             168..665
                     /locus_tag="OO309_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          201 a          195 c          226 g          156 t
ORIGIN      
        1 actcctccgg cagaagcaga aaagcttatt atgcttccat cggaaacgat gatctggcag
       61 cctgagttca cagataaaac actctccagg aaacccgggg cggttcaaag cgccagcccc
      121 gtcccgcccg atagagcgcg gcatagcggg ccccggatta cttgcgtgcg acggcagaag
      181 aagccctgaa gcgaatcagt gaactgtacg ccatcgagga tgaaatacgg ggattaccag
      241 agtcagagtg tcttgcagtc aggcagcagc gaagcaaagc gttactgacg tcgctgcatg
      301 aatggatggt ggagaagaat ggcacgctgt cgaaaaaatc cagactgggc gaagcattca
      361 gctatgtact gaatcagtgg gacgccctct gttattacag tgatgacggt ctggcggagg
      421 cggacaataa cacagcggaa agagcgcttc gtgcagtctg tctcggaaag aaaaattacg
      481 tgttcttcgg tagcgatcac ggcggcgagc gtggtgcact gctgtacggg ctgatcggca
      541 cctgccgtct gaacggtatc gatccggaag cgtatctgcg ccatattctg agcgtactgc
      601 cggaatggcc ttccaaccga gttgacgacc tcctgccatg gaaggtggtg ctaccatcag
      661 gataatcgtc aatacggtgt tcggtgaacg tttacttgga gcatcatgct tcatgaagaa
      721 aaactggcac gtcatcaacg taaacaggcg atgtataccc gaatggcagc cttcccgg
//
LOCUS       DAJTQQ010000302          758 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000302 DAJTQQ010000000
VERSION     DAJTQQ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.315"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..146)
                     /locus_tag="OO309_004335"
     CDS             complement(<1..146)
                     /locus_tag="OO309_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5849201.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQ"
     gene            complement(158..>758)
                     /gene="yiaK"
                     /locus_tag="OO309_004336"
     CDS             complement(158..>758)
                     /gene="yiaK"
                     /locus_tag="OO309_004336"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR5849202.1"
                     /translation="GICWTNSIAVMPPWGAKECRIGTNPLIVAIPSTPITMVDMSMSM
                     FSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRILPMGYWKGSGMSIVLDM
                     IATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPTRDAKLQRIMDYVTTAER
                     ADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
BASE COUNT          177 a          196 c          210 g          175 t
ORIGIN      
        1 atttgcgcat aaatattctt gccgtcgatt tcgacaacgc ccggctccag gacgttgaag
       61 ttggtagcgc gcagaaaatc gagcgccttt tcaatggcgg cgggcaaacg gcacggattg
      121 ggctgcgcga tatgtccaaa aatcatgact tatctcctca taacgcctgg attttggccc
      181 acacgctgtc atcaacagtg atgccgttac ggcggttttc ggccagcagg gtagtaaatt
      241 cgtggccggg taagcggatg gcctggtttt cgtcagcgcg ttcagcggta gtaacgtaat
      301 ccatgatacg ttgcagcttg gcatcgcggg tgggaccgtc gataagcttg tccacttcaa
      361 tggcaataaa aatttgtgaa atgccgtatt cgtcgctgtt gtcctgggtg acttcggcaa
      421 ccgatgcgcc gtcggaaagg agagtagcga tcatatccag cacaatcgac atgccagaac
      481 ctttccagta gcccatcggc aaaatgcggc gattcttctc gataacgcca ggttctttgg
      541 tcaaattgcc ctcatcatca aagccaccat cgaccgggag ctgacggcct gccaggcggt
      601 taacttctaa catgccgtaa gagaacatcg acatcgacat atcgaccatg gtgatcggcg
      661 tggaaggaat ggcgacgatc agcgggttag tgccgatgcg acactctttt gcgccccacg
      721 gcggcattac ggcgatggag ttggtccagc aaatgcca
//
LOCUS       DAJTQQ010000303          745 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000303 DAJTQQ010000000
VERSION     DAJTQQ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.316"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..159)
                     /locus_tag="OO309_004337"
     CDS             complement(<1..159)
                     /locus_tag="OO309_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR5849203.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGD"
     gene            complement(177..>745)
                     /locus_tag="OO309_004338"
     CDS             complement(177..>745)
                     /locus_tag="OO309_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5849204.1"
                     /translation="NAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAMASQR
                     AKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKMLFSHA
                     QKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVSLPDF
                     SHYELYDKPEAVQEALAKVIPFFNTHLG"
BASE COUNT          189 a          182 c          155 g          219 t
ORIGIN      
        1 atcccccata ataatctggc tattagttgg atagggtttg tgtatttttg ctggctgacg
       61 ggcaaataaa gtttgtttaa tagtttgttt atctgccagt tgattaatca catggcgggc
      121 tatctgaccg ccagcaccaa gaatcagtac atttttcata atatttctca tgaaacttat
      181 cccagatggg tgttgaaaaa aggaataact tttgccagcg cctcctgaac tgcttccggt
      241 ttgtcatata gttcataatg tgagaaatcg ggtaacgaaa ctaactgacg atcctgcgaa
      301 accatcgccc ggccatagac ttccagtcca tcacgataag caccgaaggc accgactttt
      361 tcaccaacta ctaccatcac cggctgggtc agtaacactt cagtaaatgc gaaggcatcc
      421 caggccagcg ttttctgtgc atgtgagaac aacatttttg tcgcacctcc cggttgttgc
      481 ccgcgtggtg ttttatagta gtcagttgct tcataaacat cgcgttcggt cagtccatgg
      541 gcttttgctg catccaggct ggcgggaagt aattcgttga tctgtaattc tccaccacga
      601 gcctcttttg ctcgttgtga cgccattgca ttcaatacgc caatcggatc ataattgctg
      661 aaaccttccc gaaaaagacg cccgatatta acacccgtaa tactcactac agctttaatt
      721 cgtttttctg tcagcgcggc attaa
//
LOCUS       DAJTQQ010000304          741 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000304 DAJTQQ010000000
VERSION     DAJTQQ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.317"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            1..127
                     /locus_tag="OO309_004339"
                     /pseudo
     CDS             1..127
                     /locus_tag="OO309_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            494..741
                     /locus_tag="OO309_004340"
                     /pseudo
     CDS             494..741
                     /locus_tag="OO309_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          259 a          130 c          121 g          231 t
ORIGIN      
        1 ccatatcgtg caggcaccgg tgccttcaaa acccattgca cgcagttatg ccggagcggg
       61 gcttctggcc catgttgtta ccggaaagta tgcagaccat ctgccattat accgccaaag
      121 tgatctacta ttccacacag ctatttagct ctctttttgt tgagctccca tgattctttc
      181 atttattgtt actaattatt ttatgttatc atgatgttag aacacgctct gctctgtgtc
      241 tctattttat ctatcatatt ctgaggatta actacataac aaggcgcaag atatatatac
      301 aaaaacaaat aataccgttg caataattcc ttttagtaaa ggaggtgtga ttaatttcag
      361 gggatttcag agtaaaaatt gtacaacata ttaaaatatg ttcatttcgt tataatacac
      421 acaaggaaat attaagttag ataatatatt ctaaataacc atagataaaa acgcacataa
      481 tggggaatat tttatgaaaa tacctgaagc agtaaatcat attaatgttc agaataatat
      541 tgatcttgtt gatggaaaaa cgaatccgaa taaagcaaca aaagcattac aaaaaatata
      601 ttacgcgtaa caaactcatc ttcttctggc ataagtgaaa aacatttaga tcactgtgct
      661 aatactgtga aaaacttttt aaggaagtct atagcggcgc aaagttatag caaaatgttt
      721 tctcaaggga ccagtttcaa a
//
LOCUS       DAJTQQ010000305          736 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000305 DAJTQQ010000000
VERSION     DAJTQQ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.318"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            1..736
                     /locus_tag="OO309_004341"
                     /pseudo
     CDS             1..736
                     /locus_tag="OO309_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT          157 a          222 c          198 g          159 t
ORIGIN      
        1 caatttatta caaacgggct gggcggagac tctgcccaac tggcgctatg tcgacacaca
       61 gctgccggtg cggggagatt tttcagtgcc gacacaaggt gcggaatcgc cctggggcgg
      121 cgcacaactg acgtttatcg cccgccgcga tgttacgcca cagccaccac aaacgccgca
      181 agccttactg gagtttgcta aagccaatcc cggcacggtt acttatccgc gcccaccgga
      241 ctttaccggc acggcgtttc ttgaacagtt gctgattatg ctgacgccag atcccgccgc
      301 attaaaagaa gcgccggacg atgcgacttt cgcccgtgtc actgcgccct tgtagcaata
      361 tcttgatgca ctgcatccgt atttgtggcg cgaaggaaag gatttcccgc cttcgcccgc
      421 gcggatggat gctctgctga aagccggcac attgcgcctg tcgctgacct ttaaccccgc
      481 acatgcgcag caaaaaatcg ccagcggcga tttgccggca agcagttaca gttttggctt
      541 tcgcgagggg atgattggca acgtgcattt cgtcaccatt ccagccaacg cgaatgccag
      601 tgctgcggcg aaggtagttg ccaatttcct gctctcaccc gatgcgcaac tgcgtaaagc
      661 agatcccgtt gtctggggcg atccttctgt tctcgatccg caaaaactgc ctgacgggca
      721 gcgcgaaata ttgcaa
//
LOCUS       DAJTQQ010000306          730 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000306 DAJTQQ010000000
VERSION     DAJTQQ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.319"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..>730)
                     /locus_tag="OO309_004342"
     rRNA            complement(<1..>730)
                     /locus_tag="OO309_004342"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          139 a          245 c          160 g          186 t
ORIGIN      
        1 aatcctgttt gctccccacg ctttcgcacc tgagcgtcag tcttcgtcca gggggccgcc
       61 ttcgccaccg gtattcctcc agatctctac gcatttcacc gctacacctg gaattctacc
      121 cccctctacg agactcaagc ttgccagtat cagatgcagt tcccaggttg agcccgggga
      181 tttcacatct gacttaacaa accgcctgcg tgcgctttac gcccagtaat tccgattaac
      241 gcttgcaccc tccgtattac cgcggctgct ggcacggagt tagccggtgc ttcttctgcg
      301 ggtaacgtca atgagcaaag gtattaactt tactcccttc ctccccgctg aaagtacttt
      361 acaacccgaa ggccttcttc atacacgcgg catggctgca tcaggcttgc gcccattgtg
      421 caatattccc cactgctgcc tcccgtagga gtctggaccg tgtctcagtt ccagtgtggc
      481 tggtcatcct ctcagaccag ctagggatcg tcgcctaggt gagccgttac cccacctact
      541 agctaatccc atctgggcac atccgatggc aagaggcccg aaggtccccc tctttggtct
      601 tgcgacgtta tgcggtatta gctaccgttt ccagtagtta tccccctcca tcaggcagtt
      661 tcccagacat tactcacccg tccgccactc gtcagcgaaa cagcaagctg cttcctgtta
      721 ccgttcgact
//
LOCUS       DAJTQQ010000307          700 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000307 DAJTQQ010000000
VERSION     DAJTQQ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.320"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..452
                     /locus_tag="OO309_004343"
     CDS             <1..452
                     /locus_tag="OO309_004343"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5849205.1"
                     /translation="AILVEAKTGSQRALIDYRKNPPQPYNHQKVPGNVWDFPRVRYLM
                     DEYENHPTQKPKALLKRIILASSNPGDIVLDPFAGSFTTGAVAIASGRKFIGIEINSE
                     YIKMGLRRLDVASHYSAEELAKVKKRKTGNLSKRSRLSEVDPDLIAK"
     gene            536..>700
                     /locus_tag="OO309_004344"
     CDS             536..>700
                     /locus_tag="OO309_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR5849206.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNF"
BASE COUNT          200 a          161 c          159 g          180 t
ORIGIN      
        1 atgctattct ggtagaagcc aaaaccggat cgcagcgcgc gttgatcgat tatcgcaaaa
       61 atcctccaca gccatacaat catcaaaaag taccgggtaa cgtctgggat tttccgcgcg
      121 tgcgttattt gatggatgaa tatgagaacc acccgacgca aaaaccgaaa gccttactga
      181 aacgcattat tctcgcctct tccaacccag gcgatatcgt tctcgacccg tttgccggta
      241 gctttactac cggtgccgta gccatcgcca gcggacgaaa attcattggt attgagatca
      301 acagcgagta catcaaaatg gggcttcgac ggctggatgt cgcgtcgcat tactccgcgg
      361 aagaactggc gaaagtgaaa aaaagaaaga ccggcaacct gtcaaaacga agccggttaa
      421 gcgaagttga ccccgatctc attgcaaagt aaaggaagtg taagcctagt tttcagatta
      481 ttcatttcgt gtatatttcc tgccagactt ggttaaacat gcacaggctc aggtaatgat
      541 tcgcaagtat tggtggctcg tcgttttcgc tgtcttcgtt tttctgtttg atactttact
      601 gatgcagtgg attgaactgc tgacaacaga aacagacaaa tgccgtaata tgaactcagt
      661 taatccacta aaactggtta actgtgacga actgaatttt
//
LOCUS       DAJTQQ010000308          696 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000308 DAJTQQ010000000
VERSION     DAJTQQ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.321"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..673
                     /locus_tag="OO309_004345"
     rRNA            <1..673
                     /locus_tag="OO309_004345"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          175 a          164 c          206 g          151 t
ORIGIN      
        1 ttaagtcgac cgcctgggga gtacggccgc aaggttaaaa ctcaaatgaa ttgacggggg
       61 cccgcacaag cggtggagca tgtggtttaa ttcgatgcaa cgcgaagaac cttacctggt
      121 cttgacatcc acggaagttt tcagagatga gaatgtgcct tcgggaaccg tgagacaggt
      181 gctgcatggc tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc
      241 aacccttatc ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata
      301 aactggagga aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca
      361 cgtgctacaa tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa
      421 gtgcgtcgta gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta
      481 atcgtggatc agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac
      541 accatgggag tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac
      601 tttgtgattc atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg
      661 gatcacctcc ttaccttaaa gaagcgttct ttgtag
//
LOCUS       DAJTQQ010000309          695 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000309 DAJTQQ010000000
VERSION     DAJTQQ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.322"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            62..695
                     /locus_tag="OO309_004346"
                     /pseudo
     CDS             62..695
                     /locus_tag="OO309_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          208 a          173 c          175 g          139 t
ORIGIN      
        1 actgaatttg gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc
       61 aatgaaaaaa agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga
      121 ccagaaatac acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac
      181 aagatgggtc aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctcccccat
      241 taccccggaa caaattgaaa tccgtgagct caggaaaaag ctacaacgca ttgaaatgga
      301 gaatgaaata ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg
      361 ataatcggga aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt
      421 catcgcagca gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct
      481 gtattacgca gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga
      541 agcatcgcca caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg
      601 ctcatgaaag agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt
      661 ggtcatgaac atgttgctat ccctaactac cttga
//
LOCUS       DAJTQQ010000310          695 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000310 DAJTQQ010000000
VERSION     DAJTQQ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.323"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..>695
                     /locus_tag="OO309_004347"
     CDS             <1..>695
                     /locus_tag="OO309_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HCR5849207.1"
                     /translation="HQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQ
                     LWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNGWSLAGEHRH
                     WMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCH
                     LLTSMTDAMRFPG"
BASE COUNT          174 a          203 c          198 g          120 t
ORIGIN      
        1 tcaccagatt gtgaatcgcc tggacatcat gctgccgggc aatcgcccct tcgttgcccc
       61 cagtgccgtt attcaggccc gccagcgcct gggaagtgag gctgtccgcc gcgtgttcac
      121 gaaaacagcg cagctctggc ataacgccac gccgcatccg cactggtgcg gcctgaccct
      181 gctggccatc gatggtgtgt tctggcgcac accggataca ccagagaacg atgcagcctt
      241 cccccgccag acacatgccg ggaacccggc gctctacccg caggtcaaaa tggtctgcca
      301 gatggaactg accagccatc tgctgacggc tgcagccttc ggcacgatga agaacagcga
      361 aaatgagctt gctgagcaac ttatagaaca aaccggcgat aacactctga cgttaatgga
      421 taaaggttat tactcactgg gactgttaaa tggctggagc ctggcgggag aacaccgcca
      481 ctggatgata cctctcagaa agggagcgca atatgaagag ctcagaaaac tgggtaaagg
      541 cgatcatctg gtgaagctga aaaccagccc gcaggcacga aaaaagtggc cgggactggg
      601 aaatgaagtg acagcccgcc tgctgaccgt gacgcgcaaa ggaaaagtct gccatctgct
      661 gacgtcgatg acggacgcca tgcgcttccc cggag
//
LOCUS       DAJTQQ010000311          692 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000311 DAJTQQ010000000
VERSION     DAJTQQ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.324"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..466
                     /locus_tag="OO309_004348"
     CDS             <1..466
                     /locus_tag="OO309_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HCR5849208.1"
                     /translation="RKTQEFTQQSAQYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVP
                     PAVGADIMRLFPEKSAALMYHLGANPEKTRQLLAMDGQSALIELTRLSERLTLKPRAK
                     PVSEAPLPDEPIQGHAVAANISAIEKQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            466..>692
                     /locus_tag="OO309_004349"
     CDS             466..>692
                     /locus_tag="OO309_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCR5849209.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSV"
BASE COUNT          196 a          187 c          177 g          132 t
ORIGIN      
        1 tcgcaaaact caggagttca cccagcaatc agcgcaatac gtcgaggcgg cccgtaagca
       61 ctacgaggca gcggaaaagc tcaatatccc tgactatcag gagaaagagg atgcattcat
      121 gcaactggtg ccgccagcag tcggtgccga catcatgcgc ctcttcccgg agaaatccgc
      181 cgctctcatg tatcaccttg gtgctaatcc tgagaaaaca cgccagttgc tggcgatgga
      241 cgggcaatcc gcgctgattg aactcactcg actgtcagaa cgtttaactc tcaagcctcg
      301 agccaagcct gtttcagaag ccccgttacc tgatgaaccc attcagggac atgctgttgc
      361 tgcaaatatc tctgcgattg aaaagcagat ggaggcggca gcaaacaaag gggatgtaga
      421 gacgtaccgc aagctcaagg cgcaactgaa taaaggaatt cgataatggc attaaatgaa
      481 ggtcaactgg tcacgtatgc tctggatgaa atcatcggaa ccgtccagaa cctgacgcca
      541 atggcgtcaa aagtgacaaa atacccccct ccggcagaat ccatgcagcg ttcaagcaac
      601 accgtgtgga tgcctgttga gcaggaagcg ccaacccaga ctggctggga tttaactggc
      661 aacgcaaccg ggattctgga actctccgtg aa
//
LOCUS       DAJTQQ010000312          688 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000312 DAJTQQ010000000
VERSION     DAJTQQ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.325"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..>688
                     /locus_tag="OO309_004350"
     CDS             <1..>688
                     /locus_tag="OO309_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR5849210.1"
                     /translation="RLPIHEACSHVIWDANSKQMDKSDAKHCTVINALSRNGWKEFAE
                     DYGIDPDTLPSFQNPNDTWLFPWVSNDVVYVAEYYEVEEEKEKVFIYRDPLTGEPVSY
                     YQQDIKDVIDDLANRGFIKVAERKVKRRRVYKSIITCTQILKDREKIAGEHIPIVPVY
                     GEWSFAGDKECYEGVVRLTKDGQRLRNMIMSFNADIVARSPKKKPTFFPEQIEGYEYM
                     YGGNDDYPYYL"
BASE COUNT          188 a          162 c          189 g          149 t
ORIGIN      
        1 gacgcctgcc aatccatgaa gcctgctcac acgtcatatg ggacgccaac agcaagcaga
       61 tggataagag cgacgctaag cactgcacgg tgattaacgc cttgtcgcgc aatggctgga
      121 aagagttcgc agaggattac ggtattgacc cggataccct gccatctttc cagaatccta
      181 acgatacatg gctattcccg tgggtgtcga atgatgtcgt ctacgtcgct gagtattacg
      241 aggtagaaga ggagaaggag aaggtcttca tctaccgcga cccgctgaca ggtgagccgg
      301 tcagctatta ccagcaggat atcaaagacg tcatcgacga cctggctaat cgtggattca
      361 ttaaggtagc agagcgcaag gtgaagcgtc ggcgtgtgta taagtcgatc atcacctgta
      421 cgcagatact gaaagaccgc gagaagatag ccggagagca tattccaatc gttccagtgt
      481 atggcgaatg gtcattcgct ggtgacaagg agtgctacga gggtgtggta aggctgacga
      541 aagacggtca acgccttcgt aacatgatta tgtcgttcaa cgccgatatt gttgctcggt
      601 caccgaagaa gaaaccgacc ttcttccctg agcaaatcga aggctacgaa tacatgtacg
      661 gcggaaatga tgactatccg tactatct
//
LOCUS       DAJTQQ010000313          686 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000313 DAJTQQ010000000
VERSION     DAJTQQ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.326"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            1..686
                     /locus_tag="OO309_004351"
                     /pseudo
     CDS             1..686
                     /locus_tag="OO309_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
BASE COUNT          159 a          174 c          202 g          151 t
ORIGIN      
        1 tctccctcgt tgcccggcaa catggtgtag cagccagcca gttatttctc tggcgtaagc
       61 aataccagga aggaagtctt actgctgtgg ccgccggaga acaggttgtt cctgcctctg
      121 aacttgctgc cgccatgaag cagattaaag aactccagcg cctgctcggc aagaaaacga
      181 tggaaaatga actcctcaaa gaagccgttg aatatggacg ggcaaaaaag tggatagcgc
      241 acgcgccctt attgcccggg gatggggagt aagcttagtc agccgttgtc tccgggtgtc
      301 gcgtgcgcag ttgcacgtca ttctcagacg aaccgatgac tggatggatg gccgccgcag
      361 tcgtcacact gatgatacgg atgtgcttct ccgtatacac catgttatcg gagagctgcc
      421 cacgtatggt tatcgtcggg tatgggcgct gcttcgcaga caggcagaac ttgatggtat
      481 gcctgcgatc aatgccaaac gtgtttaccg gctcatgcgc cagaatgcgc tgttgcttga
      541 gcgaaaacct gctgtaccgc catcgaaacg ggcacataca ggcagagtgg ccgtgaaaga
      601 aagcaatcag cgatggtgct ctgacgggtt cgagttctgc tgtgataacg gagagagact
      661 gcgtgtcacg ttcgcgctgg actgct
//
LOCUS       DAJTQQ010000314          682 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000314 DAJTQQ010000000
VERSION     DAJTQQ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.327"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            65..538
                     /locus_tag="OO309_004352"
     CDS             65..538
                     /locus_tag="OO309_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5849211.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          225 a          122 c          142 g          193 t
ORIGIN      
        1 gctggctaac accccattat aaggggtacg aataaagtaa cgggggggag atgaacccta
       61 tgcaatgtcc tggttaatat ctcagtgtgc tcatcagtgc actgataata aaaagacgga
      121 gacagatgcc atttacgata aagttcggag ttcttatttg ctttcatgta ttctgaaaaa
      181 aaacaaaaat gttgggctta ttcttcatgc accatcattt gtttctgtgt ctgaaaaaat
      241 cgccagaatt gtgatggcaa attactcccg taactggagt aattccgagt tggccagtgc
      301 ggttttaatg agtgaatctt ctttaaaaag aagaatgtac aaagaggtcg gttctataag
      361 tacatttgta cataaaataa agcttacaga agctatcagg aaattacgaa ggacaaatac
      421 tcccatttct gtcatatcat ctgagttagg ttacagttca ccatcgtatt tttctaaagt
      481 cttctttaaa tatttaaaga cataccctca aaacataagg aagaaaaatg gtcggtaatg
      541 tcaccaatta ggaggacttg cagagccgct tttttacgat actgagatca aacagacata
      601 atctatgcgg agatttacag cagggcatgg ttcacaaatc tgacagcgat gaattatcgg
      661 cactgcgggc agaaaatgct cg
//
LOCUS       DAJTQQ010000315          660 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000315 DAJTQQ010000000
VERSION     DAJTQQ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.328"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
BASE COUNT          192 a          148 c          150 g          170 t
ORIGIN      
        1 ttttccactt gaagacctca ttaaaaataa tggcaaaaaa atcaggtctg ataagaaaca
       61 cttcttagaa tgcaatgaag acaaataaca acaatctgca tattgccacc aaccacagat
      121 gtggcatcgt tatcagcgca cttgattttc tttatattca ctgacgagct tgccggggca
      181 cctttgtttg ccggtcattc tggtatcatg cagatattta cggcgtacaa gcccactagc
      241 atgacattgt aataaggcct gagcctaacg ctttaatgtg tgtcatacac tcagtggcgt
      301 aatcgttgac aatatggtgc atgtaatcgt aagcgtccag cgaaatccat ctggtcataa
      361 tctgaagcat ccgacaaagt ggtgtccacc aaataagtga tctgcgccca atatcctgag
      421 ccggaacttt gacaaaggcc aacgaaaaat gggagcggat gttacagagt tctcggtaca
      481 ggggaaaagt ggtacctgtc gccagttctc cgatctgttt aaccgggaaa ttatctccta
      541 cagcttgtca gaaaggtcgg tgatggagca tcgttaatac gatgctatac gatgcattca
      601 cagtgcccgg ccagaggatg ccccgtcgct gcatatggat cagggttggc aatatcgaat
//
LOCUS       DAJTQQ010000316          655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000316 DAJTQQ010000000
VERSION     DAJTQQ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.329"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..>655
                     /locus_tag="OO309_004353"
     CDS             <1..>655
                     /locus_tag="OO309_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR5849212.1"
                     /translation="IAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     "
BASE COUNT          131 a          164 c          158 g          202 t
ORIGIN      
        1 gcattgcccc tggcataatg atgggcgcaa cgctgatgct tacctggtgg cggcaggcca
       61 gtcgccttaa tttaccccgc cagcaaaaag caacgatgca ggaaatctgg cactcttttg
      121 tctccggtat ctgggcgctg tttcttccag tcatcattat tggcgggttt cgctccggtc
      181 tgtttacccc taccgaagcc ggggccgttg ctgctttcta tgcgctgttt gtcgccacag
      241 ttatttaccg tgaaatgacc ttcgccacac tttggcatgt actcattggc gcggcgaaaa
      301 ccacctcagt ggttatgttt ctggtggcct cggcacaagt ttccgcctgg ctgattacca
      361 ttgctgaact gccgatgatg gtttctgatt tactgcaacc gttggtcgat tcaccgcgtc
      421 tgctgtttat cgtcattatg gtggctatcc tgattgtcgg catggtcatg gatttaacgc
      481 cgacggtatt aattcttacc ccggtattaa tgcctttagt taaagaggca ggaatcgatc
      541 cgatttattt cggtgtcatg tttatcatta actgctcaat cggcttaatt acaccgccta
      601 tcggtaatgt acttaacgtt atttcagggg tggcaaaact caaattcgat gatgc
//
LOCUS       DAJTQQ010000317          653 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000317 DAJTQQ010000000
VERSION     DAJTQQ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.330"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..216
                     /locus_tag="OO309_004354"
     CDS             <1..216
                     /locus_tag="OO309_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 family protein"
                     /protein_id="HCR5849213.1"
                     /translation="VYSDFWESVAGKTCGDWYRLPTQVIRKPLSDIASKKRSGYRKRY
                     ALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            281..>653
                     /locus_tag="OO309_004355"
     CDS             281..>653
                     /locus_tag="OO309_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5849214.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEI"
BASE COUNT          202 a          119 c          145 g          187 t
ORIGIN      
        1 gtctatagtg atttctggga gtctgtagca ggaaaaacct gtggtgactg gtacagatta
       61 ccaacccagg tgattcgaaa gccgctaagc gatatagcca gtaaaaaacg ctctgggtac
      121 cgaaaacgat acgctttgct ggattatatc catgaaacta ctatccgctc tttggatgca
      181 tatcctgtac actcagaaca tcagaattta aattaaggtt tgatgtattc tctgaataaa
      241 acagtaatga taattttggt agaggcattc gcactaaata atgaaaaaat ataaatctga
      301 gtttattcct gaatttaaga aaaattacct ttcccctgtt tactggtcta catggttcct
      361 tttgggaatg attgcaggta tttcaatgtt tcccccttca ttcagagatc ctgtcttggc
      421 aaaaataggg cgttgggcgg gcagattgag caaaaaagct cgtcgcaggg cgacgattaa
      481 tttatcgctt tgtttcccag aaaagagtga tacagaacgg gaaataattg tcgacaaaat
      541 gtttgtcaca gcattgcaat ccatagtgat gatggcagaa ctggcaattc gtggtccgga
      601 aaagttccag aaacgagtgt tctggaaagg cctcgaaatt cttgaggaaa ttc
//
LOCUS       DAJTQQ010000318          643 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000318 DAJTQQ010000000
VERSION     DAJTQQ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.331"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            38..>643
                     /locus_tag="OO309_004356"
     CDS             38..>643
                     /locus_tag="OO309_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR5849215.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYP"
BASE COUNT          138 a          174 c          186 g          145 t
ORIGIN      
        1 cggttcactg gccggacgga gtcgccccgc ccttctgatg ctcccccgtt ttgccgatat
       61 ttttcagcag ggaaaccgct ggcttaactg gctggagaaa caaccggaag gggcagtccg
      121 tccggtggta attgaatcag tgacaaaaat catggcctgc gggaccacac tgatgggcta
      181 cacgcagtgg tgttgttcat caccggactg ttgccataca aaaaaggtct gcttccggtg
      241 taaaagccgc tcctgcccgc actgcggggt gaaggctggc gcacagtgga tacagtatct
      301 gctgagcctg gtcccctact gcccgtggca gcatattgtg ttcacacttc cctgccagta
      361 ctggtccctg gtgttccaca accggtggtt actggcagag atgagccgca ttgcagcgga
      421 tgtgatactg gaaatctgcc atcaggcaga tgtggagccg gggatattca cggtgatcca
      481 cacatggggg cgtgaccagc agtggcatcc gcatatccat ttatcgacaa ctgccggtgg
      541 tgtgacgtcg ggccacacct ggaaaaatct tcatttttac gcccgtaagg tgatgagcat
      601 gtggcgctac cggataacac ggttgctgtc acggaaatac cct
//
LOCUS       DAJTQQ010000319          638 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000319 DAJTQQ010000000
VERSION     DAJTQQ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.332"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..>638
                     /locus_tag="OO309_004357"
     CDS             <1..>638
                     /locus_tag="OO309_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HCR5849216.1"
                     /translation="GKPYSGPQEVADADGLSVRISPKGVIQFQYRYRWHGKPNRLGLG
                     RYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLDYWFDNYVSTTLR
                     EKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRRARQVLSQLRSAI
                     SWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIERSRAST"
BASE COUNT          165 a          160 c          165 g          148 t
ORIGIN      
        1 atggtaaacc atattcgggc ccacaagaag tggctgatgc cgatggcctc agcgtacgaa
       61 tttcaccgaa gggggtcatc cagttccagt accgctatcg ctggcatggc aagcctaatc
      121 gacttgggct tggtcgatac ccatccctgt ctttgaagga tgccagacag atcactgctg
      181 acttgcgaaa gctctatttc tcaggaacgg atccacgcac ctattttgaa gagaaggtgg
      241 agaactccat gacggtcgcc cagtgtctcg actactggtt cgacaactac gtctctacaa
      301 ctctcagaga aaagacccag gcactttatc gatcaacggt tatgaagcgc atgcatgacg
      361 ccttccctaa tcgtccggca tcttctatca cggttaagca atgggttgac ctgcttaccg
      421 aagaagaaaa agataatcca cgccgagcaa ggcaggtgct aagtcaacta agatcagcaa
      481 ttagttggtg catgcggcgt cagttgatag atagttgcgc aattatgagc atccagccaa
      541 gggacttcgg ctcccgcgct gaggtagggg atcgggtact gtcgtatcac gaactggcta
      601 agatttggct tgctattgaa agaagccgtg cgtctacg
//
LOCUS       DAJTQQ010000320          626 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000320 DAJTQQ010000000
VERSION     DAJTQQ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.333"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..121)
                     /locus_tag="OO309_004358"
     CDS             complement(<1..121)
                     /locus_tag="OO309_004358"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008499737.1"
                     /note="catalyzes the formation of putrescine from
                     agmatine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR5849217.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGV"
     gene            complement(257..>626)
                     /locus_tag="OO309_004359"
     CDS             complement(257..>626)
                     /locus_tag="OO309_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849218.1"
                     /translation="YQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVNAGD
                     SLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNALPP
                     CSGQAFPADNNGYAPPPCRK"
BASE COUNT          162 a          152 c          158 g          154 t
ORIGIN      
        1 gcacgccagt aatcacccag tctgcatcgc tgtcatacgg ctggaagttc atcggcaggc
       61 gtaaaaaacc aaaggcattg gaaaccagtg agttatcgta ttgatgacct aaggtgctca
      121 tggatggact cctattacaa agtcgatata aaaaaaccct tccgcgtcgt taggcccgac
      181 gaggaagggt tggatttgcg aaacacaagt ttgtgctaat tatcgcctta aatgggcgag
      241 gcatcaagcg aggttgctat ttccgacacg gtggcggcgc atagccattg ttatctgccg
      301 gaaaggcttg tccagagcac ggcggcagcg cgttttgcgc cgggccttgt aattcaaagg
      361 cggcaaagcc gattaccccg gtattacgca catcgccttg cgccgaattg gctgcgtagg
      421 aatcagcaac gttggcgaac tgaaaagccg cttcagtatg tttatctttg cggaatcctt
      481 taatcactaa cgaatctccg gcattcacaa tatagccgtt gttattcagc gaaccttgct
      541 tgccgttcag cacatccagg ccatcagccg tagcaacgat ctcgtagttg gtgttacgac
      601 tgtaattacg gacatataat tgataa
//
LOCUS       DAJTQQ010000321          600 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000321 DAJTQQ010000000
VERSION     DAJTQQ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.334"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..>600
                     /locus_tag="OO309_004360"
     CDS             <1..>600
                     /locus_tag="OO309_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5849219.1"
                     /translation="KPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMIIDSISHF
                     WTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAGYEYDFFE
                     GDDGKRQLAKTGIKMKAETETGYEPSIRIQMEKQMNIETGQVWRTARILKDRSTRIDG
                     QVFSNPTFKNFLPHIESLNLGGEHPGIDTSRDNSELFAN"
BASE COUNT          181 a          137 c          143 g          139 t
ORIGIN      
        1 ttaaaccccg ctttgatgcg gaaaatatcg aattatttac ggcaaaaacc cgctcgtttg
       61 ttgatctgct tgaagccatt aatgaagcgg aaagcagcgg ttccgtaatg atcatcgact
      121 ccatcagtca cttctggacc ggattatgcg acgagtatgc cagacgccga aaccgtaaac
      181 gaggactgga attttcagac tgggcgtggc tcaaacagga gtggcgacgc tttaccgatc
      241 gatttgttaa cagtcaggcg cacattatta tgtgcgggcg tgcaggatat gagtatgact
      301 ttttcgaagg tgatgatggc aaacgccagt tggcaaaaac cggtataaaa atgaaagcag
      361 aaacagaaac cggttatgaa ccctccatcc ggatccagat ggagaagcaa atgaacatag
      421 aaaccggtca ggtgtggcgc accgccagaa tactaaaaga tcgctccacc cgtattgatg
      481 gacaggtatt ttcaaaccca acatttaaaa actttcttcc tcatatcgag tctcttaacc
      541 tgggaggtga acaccccggc atcgatacct cacgggataa cagtgaatta tttgccaacg
//
LOCUS       DAJTQQ010000322          598 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000322 DAJTQQ010000000
VERSION     DAJTQQ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.335"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..>598
                     /locus_tag="OO309_004361"
     CDS             <1..>598
                     /locus_tag="OO309_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5849220.1"
                     /translation="GATKGQLEAWQENAMFDTGRYRRKKIRYRDEVTGKMITRDNPPI
                     PGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYKAWLLWCYSGSICWEYQITITH
                     WAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAELFGGEGYEYQELALLAGVTTK
                     NWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKAA"
BASE COUNT          173 a          115 c          173 g          137 t
ORIGIN      
        1 gtggggcgac aaaaggtcag cttgaagcct ggcaggagaa tgccatgttc gatacagggc
       61 gttacaggcg taaaaaaatc cggtaccgcg atgaggtgac cggaaaaatg ataacgcggg
      121 ataatccacc aatcccggga aaacaatcac tggcgaaggg ggcgtcaatt cctctggtca
      181 gtcaggttgc gttttcgaca tcatcgtggc ggcgggctgt tctgtctctt gaagagcact
      241 ataaagcctg gttgttgtgg tgttacagcg gaagtatttg ctgggagtat cagatcacca
      301 taacccactg ggcgtgggaa gagtttaagg ctcattctgg cactaggaaa attgcagaga
      361 aaacacagga acgcctgaaa aaattaatct ggctggcggc gcaggcggta aaagcagaac
      421 tttttggtgg ggaaggttat gaatatcagg agctggcatt actggcggga gtaacaacca
      481 aaaactggtc caaaacattt actggtcact gggttgtaat gaaacacatt tttcaccgac
      541 tggatagtga ggctttattg tttgtgatga gaacacgttc agaacaaaaa gcggcatt
//
LOCUS       DAJTQQ010000323          588 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000323 DAJTQQ010000000
VERSION     DAJTQQ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.336"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..>588)
                     /locus_tag="OO309_004362"
     CDS             complement(<1..>588)
                     /locus_tag="OO309_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5849221.1"
                     /translation="RLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYL
                     GGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKQGPYE
                     FICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMR
                     IMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIA"
BASE COUNT          139 a          168 c          160 g          121 t
ORIGIN      
        1 gcaataccct gcagtttcgg gtgatcgctg agatatttca gggaggcttt gtagtcttcc
       61 agcaacagtt cagcaaagtc catttcgaag ccgcgacgac acataatgcg catcaccacg
      121 atgtcggtgg cttcaccgcc gagagtgaag gccggaacct gccagccgcg cagacgcaga
      181 cgttcagaga ggtcgtacag ggtgtatccc ggatcttcac catctttcag tttgaagcaa
      241 accgccggga tgccttcgtc cgggcgaccc gtacagatga actcatacgg cccctgtttg
      301 gcgatttcat ccgccagata agcggcaacc tggtaagagg cgttctgtac tttggtatag
      361 ccttcacgac cgaggcgcag gaattcatag tactgtgcaa ttacctgacc cgccgggcgg
      421 gagaagttga tggcaaaagt accaatttga ccacccaggt agtcaacgtt gaacaccagt
      481 tcctgcggca gcgcttcttc gtcacgccag ataacccagc cgcagcccag cggagccaga
      541 ccgaatttat ggcctgaagc actgatcgat ttcacacgcg gcaggcgg
//
LOCUS       DAJTQQ010000324          588 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000324 DAJTQQ010000000
VERSION     DAJTQQ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.337"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
BASE COUNT          181 a          141 c          116 g          150 t
ORIGIN      
        1 tgaaaaaaca cgcacgacta attgacaata attttcattt ccattaaaaa agatcgcgta
       61 gcattcttaa ttcatgagga actttacccg ccagcaatct gagtagcgaa agctgttcat
      121 ccccaatgtt tttgctggcg ggtccttttt tctacggtcc tcctccccgg aggggggcat
      181 ttcattattt ttctaatcgt tactggtgag aaggcggaag aaaaagccag ttcttcgccc
      241 ctaaataaca gcatgattcc agtccatgca aaatctgatt tccagctcac acataccgag
      301 cgctgtacaa aaaaccgcca gccaaggcag ttaagagtgt gttcccgggg tttgcttaga
      361 atattttgta agttgtccga agagatattt acaacaccag aatgatgcat tatcagtccc
      421 ctgccagagg ttcactacgt aacctgaccg acaatgcacc attctggtgc tgaaaaaaga
      481 gcactggaac tgcaacaaaa caacatcaca gaacaggaga tggtaaggag tcagacattc
      541 ccacacaata ttgtgtcagt gcaaataaca accttcgtct caaatctc
//
LOCUS       DAJTQQ010000325          587 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000325 DAJTQQ010000000
VERSION     DAJTQQ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.338"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..>587)
                     /locus_tag="OO309_004363"
     CDS             complement(<1..>587)
                     /locus_tag="OO309_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR5849222.1"
                     /translation="LSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVYVAE
                     YYEVEEKKEKVYIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRVYKS
                     IITCTHILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIMSFN
                     ADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYL"
BASE COUNT          139 a          154 c          130 g          164 t
ORIGIN      
        1 agatagtacg gatagtcatc atttccaccg tacatgtatt cgtagccttc gatttgctca
       61 gggaagaagg taggtttctt cttcggtgaa cgagcaacaa tatcggcgtt gaatgacatg
      121 atcatgttac gaaggcgttg accgtctttc gtcagcctta ctacaccttc gtagcactcc
      181 ttgtcaccag caaatgacca ttcgccatac acaggaacga ttgggatatg ctctccagct
      241 atcttctcgc ggtctttcag tatatgcgtg caggtgatga tcgacttata cacacgccga
      301 cgcttcacct tgcgctctgc taccttaatg aatccacgat tagccaggtc gtcgatgacg
      361 tctttgatat cctgttggta atagctgacc ggctcacctg tcagcgggtc gcggtagatg
      421 tatactttct ccttcttctc ttcgacctcg taatactcag cgacgtagac gacatcattc
      481 gacacccacg gaaacagcca tgtatcgttc ggattctgga aagatggcaa ggtgtcagga
      541 tcaataccgt aatcctctgc gaactctttc cagccattgc gtgacaa
//
LOCUS       DAJTQQ010000326          582 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000326 DAJTQQ010000000
VERSION     DAJTQQ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.339"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..306
                     /locus_tag="OO309_004364"
     CDS             <1..306
                     /locus_tag="OO309_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="with PhnCE is involved in the transport of
                     phosphonates; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5849223.1"
                     /translation="NLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASSDLQLV
                     PIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVSKAVQ"
     gene            412..>582
                     /locus_tag="OO309_004365"
     CDS             412..>582
                     /locus_tag="OO309_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HCR5849224.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMATFAVMTPTY"
BASE COUNT          148 a          160 c          167 g          107 t
ORIGIN      
        1 aatctgtccg aaactaccaa agacaagatc tacgacttct ttatgaacta cggaaaaacg
       61 ccggaagaga aagcggtgct ggaacgcctg ggctgggcgc cgttccgcgc ttccagcgac
      121 ctgcaactgg tgccgattcg ccagctcgca ctgtttaaag agatacaaag cgtgaaaggc
      181 aataaagggc tgaatgagca ggacaagctg gcgaaaacca ccgagattca ggcgcagctg
      241 gatgacctgg accgcctgaa caacgcgtta agcgcgatga gttcggtgag taaagcggtg
      301 cagtaaatcg taggtcggat aagacgcccc ggcgtcgcat ccgacaatgt gtaggcgttg
      361 atgccggatg cggtgcaagc accttatccg gcctacagac cggagccaaa catgcaaacc
      421 atcaccatcg ccccacccaa gcgtagctgg ttctcgcttc tgagctgggc cgttgttctc
      481 gacgtgctgg tcgtctcgtg gcagggcgcg gaaatggccc cgcttacgct gattaaagac
      541 ggcggcaaca tggcaacctt cgcggtaatg actccaactt ac
//
LOCUS       DAJTQQ010000327          576 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000327 DAJTQQ010000000
VERSION     DAJTQQ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.340"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            65..576
                     /locus_tag="OO309_004366"
                     /pseudo
     CDS             65..576
                     /locus_tag="OO309_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          119 a          151 c          164 g          142 t
ORIGIN      
        1 cataattgcg gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc
       61 tccagtggct tctgtttcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg
      121 taacggcaaa agcaccgccg gacatcagcg ctatctctgc tctcactgcc gtaaaacatg
      181 gcaactgcag ttcacttaca ccgcttctca acccggtacg caccagaaaa tcattgatat
      241 ggccatgaat ggcgttggat gccgggcaac cgcccgcatt atgggcgttg gcctcaacac
      301 gattttccgc catttaaaaa actcaggccg cagtcggtaa cctcgcgcat acagccgggc
      361 agtgacgtca tcgtctgcgc ggaaatggac gaacagtggg gatacgtcgg ggctaaatcg
      421 cgccagcgct ggctgtttta cgcgtatgac aggctccgga agacggttgt tgcgcacgta
      481 ttcggtgaac gcactatggc gacgctgggg cgtcttatga gcctgctgtc accctttgac
      541 gtggtgatat ggatgacgga tggctggccg ctgtat
//
LOCUS       DAJTQQ010000328          544 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000328 DAJTQQ010000000
VERSION     DAJTQQ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.341"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..398)
                     /locus_tag="OO309_004367"
     CDS             complement(<1..398)
                     /locus_tag="OO309_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR5849225.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPV"
     gene            complement(401..>544)
                     /locus_tag="OO309_004368"
     CDS             complement(401..>544)
                     /locus_tag="OO309_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="membrane-spanning protein involved in uptake of
                     2,3-diketo-L-gulonate; part of the tri-partite
                     ATP-independent periplasmic transport system (TRAP)
                     YiaMNO; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease DctQ"
                     /protein_id="HCR5849226.1"
                     /translation="SPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPP
                     QGA"
BASE COUNT          155 a          157 c          143 g           89 t
ORIGIN      
        1 accgggtagt tggcgctgcg catcatcggc accagtagag cggcaacggc ggcggtatcc
       61 gcaacggcag aaccagaaag gctcgccata atcatagctg ccagcacgcc gacgtagccc
      121 agcccgcctg gtttatgccc caccagtttc atcggcaggt caacaatgcg ttttaacaag
      181 ccgcccgcat tcatgatttc acccgccagc acaaagaacg gaatcgccag gagggagaag
      241 ctatcggctc cgttcaccag cgtttgtgcc atgatctgga catcaaacat gtccagccag
      301 aacattaacg ccgccccgca caacaacagt gcccaggcaa taggcaaacc gatagcaata
      361 ccacccaaca gacagcccag aaaaatcagc acagccatga ttaagctcct tgcggtggcg
      421 acgttagaga attgctacgc gtgatgagtt gatataaatg acgcagttca aagaatgcga
      481 taacgaggct ggtgggcaga caagcggcat acatcaggcc gatgggcaaa ccgaggatcg
      541 gtga
//
LOCUS       DAJTQQ010000329          544 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000329 DAJTQQ010000000
VERSION     DAJTQQ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.342"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..230
                     /locus_tag="OO309_004369"
     CDS             <1..230
                     /locus_tag="OO309_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR5849227.1"
                     /translation="GENALRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEP
                     EDWLREVIEKLNDWPSNQVHELLPWNFSSVK"
     gene            239..544
                     /locus_tag="OO309_004370"
                     /pseudo
     CDS             239..544
                     /locus_tag="OO309_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          149 a          131 c          144 g          120 t
ORIGIN      
        1 tcggtgaaaa cgcgttacga tcggtggcgg ttggaagaaa aaattatctc tttttcggct
       61 cagacaaggg aggagaaagt gcggcgatca tctacagtct gctggtcacc tgcaaacaga
      121 acgaagtgga gccggaggac tggttgcgcg aagtgatcga gaagctcaat gactggccgt
      181 cgaaccaagt gcatgaactg ctgccctgga acttctcgtc tgtaaaataa tccttacgct
      241 acgtacttct cggggcgctt acgttgatgc agaagccata tgtgaagcgg cttcacgacc
      301 atcaatgcgt tttgtccagc caagaactga gacccagcaa gcaataaggg cattacaccg
      361 ggtcagagaa tcgctcatca gagataaggt gaaaacaacc aaccagatcc acggtttcct
      421 gctggaattt ggaatcagcc tgccgacagg ggatgccgta ataaaacggt tgtctctcgt
      481 attggctgaa cacgaaatcc ccgaatatct gagccgcctt ttggtgaggt tgcataccca
      541 ctac
//
LOCUS       DAJTQQ010000330          533 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000330 DAJTQQ010000000
VERSION     DAJTQQ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.343"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..>533)
                     /locus_tag="OO309_004371"
     CDS             complement(<1..>533)
                     /locus_tag="OO309_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR5849228.1"
                     /translation="ATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQDN
                     LATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLNDI
                     RGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEMM
                     REYANKQLVMMGLKKPET"
BASE COUNT          115 a          146 c          124 g          148 t
ORIGIN      
        1 gtttctggtt tcttcagccc catcatcacc agttgcttgt tcgcgtactc tcgcatcatc
       61 tcgacgcctt taccgtcaag cagcgtgaag tattgcagca tcagcatctg gaactctgga
      121 gtaccttgtg gaacctttgt tagcaactcc tgaatctctg cgcgattctg ttccttcatg
      181 ctctggaagg atggtccaac gtctgtatag cactcatagc gaccgcgaat gtcgttgagt
      241 gtgaccacat tgccggactg gtaatcgaca acttgcgcat agagttgaac gtctttctcg
      301 cttccgtctt caagagtcag cgttacatgg cgaggaacgt cataaatatc gttgaccatt
      361 gaggcataaa tctcgccatc acgtcgcatt gcggtagcca ggttatcctg aaacacgtat
      421 gtctcaaggt ctgcccgcat gttcagttga ttgacggtat cgaaagcgac ctgaccattt
      481 gccgcctgcg catcaacgcc aagactagcc acctctttca ctgcgttggt ggc
//
LOCUS       DAJTQQ010000331          531 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000331 DAJTQQ010000000
VERSION     DAJTQQ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.344"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            117..>531
                     /gene="curA"
                     /locus_tag="OO309_004372"
     CDS             117..>531
                     /gene="curA"
                     /locus_tag="OO309_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR5849229.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAY"
BASE COUNT          118 a          120 c          164 g          129 t
ORIGIN      
        1 ggattcctcg atgaaattat tgcgtgttgc agaggggatg tgacggctgc aaacaaaacc
       61 aatcccgtga gcctgctact attgatggat tcatgccaaa agcacaggag agcattatgg
      121 ggcaacaaaa gcagcgtaat cgacgttggg ttctggcctc gcgtccacat ggcgcaccgg
      181 ttccggagaa tttccgtctt gaagaagatg atgtcgccac accgggtgaa ggacaggtgt
      241 tactgcgcac agtttatttg tccctggacc cgtatatgcg tggacgtatg agcgatgagc
      301 catcttattc accgccggtt gatattggcg gcgtgatggt cggcggtacg gtgagccgtg
      361 tcgtggagtc gaatcatcct gattatcagc ctggcgactg ggtgctgggc tacagtggat
      421 ggcaggacta tgacatatcc agtggtgatg acctggtgaa acttggcgat catccgcaaa
      481 atccatcgtg gtcgctgggt gtgctgggga tgccaggctt taccgcttat a
//
LOCUS       DAJTQQ010000332          528 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000332 DAJTQQ010000000
VERSION     DAJTQQ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.345"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..380
                     /locus_tag="OO309_004373"
     CDS             <1..380
                     /locus_tag="OO309_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR5849230.1"
                     /translation="QQPQQPSAEQIQAQGILLQGQAELLKAENQQAQIQVEAAKVEAQ
                     NQLNAAKIAEIFNNMDLDKQAELREYLKLVGQFQQQRSKDARANAELLLKDADQTHSQ
                     RMDFANLMRQVQTPSGGVAETPQ"
     gene            394..>528
                     /locus_tag="OO309_004374"
     CDS             394..>528
                     /locus_tag="OO309_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HCR5849231.1"
                     /translation="MNPTTDIQASEELTLPGNHAAASADGLVVDNANDNAGQEEGFEI
                     V"
BASE COUNT          159 a          144 c          129 g           96 t
ORIGIN      
        1 aacagcagcc gcagcagcca tcagcagagc aaattcaggc gcagggtatc cttctgcaag
       61 gtcaggctga attgctcaag gcagagaacc aacaggcgca gattcaggtt gaagccgcca
      121 aggttgaagc tcaaaaccaa ctcaacgccg cgaagattgc agaaatcttc aacaatatgg
      181 acctcgacaa gcaggcagaa ctgcgtgagt acctcaagct cgtaggtcaa ttccagcaac
      241 agcgcagcaa agacgctcgc gctaacgctg agctgcttct taaggatgca gaccagactc
      301 attcacaacg catggatttc gcgaatctta tgcgtcaagt tcaaaccccc tccggcggag
      361 tagccgagac acctcaataa gagagagtta atcatgaacc caaccaccga cattcaggct
      421 tctgaagaat taaccctgcc cggcaatcat gcagcggcat ctgctgatgg cttagttgtc
      481 gataatgcca acgacaacgc aggtcaggaa gaaggcttcg agattgtc
//
LOCUS       DAJTQQ010000333          528 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000333 DAJTQQ010000000
VERSION     DAJTQQ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.346"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..>528)
                     /locus_tag="OO309_004375"
     CDS             complement(<1..>528)
                     /locus_tag="OO309_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR5849232.1"
                     /translation="LLLPATPESCDFLHQIKIGEWIHADFKRVRNYAFHKRFFKLLQL
                     GFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQYLNTVATCRTRDT
                     ALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDETEFQQVYKSVLNVL
                     WNWILFRKFSSPEEV"
BASE COUNT          137 a          138 c          123 g          130 t
ORIGIN      
        1 cgacttcctc cggagaggaa aatttacgga acaggatcca gttccacagc acattcagta
       61 cagatttata aacctgctga aactcggttt cgtccatgtt cgcaaatgcg atagattttg
      121 ccctgcgccc acgactacca tcaggataaa tatgctcggt gtaaaatccg gcctgaatgg
      181 ttacccactc gcggaaagcg tcaaacgact ttagcaatgc cgtatcccgg gttctgcatg
      241 tcgcaactgt attaaggtat tgctctgcgg catcactcag ggctggagtg tgttcccgac
      301 ctactgattc gcacaggtaa tcaacgaagc ctgataccag ttttcgttcg cgaggcgtga
      361 tcgccccacc gaccggagtc cagtaatcaa atcccagttg caggagtttg aaaaaacgct
      421 tgtggaatgc gtagttacgc acacgcttaa agtctgcgtg tatccactca cctattttga
      481 tttgatgcaa aaaatcgcaa ctctccggcg tcgccgggag aagtaatc
//
LOCUS       DAJTQQ010000334          528 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000334 DAJTQQ010000000
VERSION     DAJTQQ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.347"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            83..>528
                     /locus_tag="OO309_004376"
     CDS             83..>528
                     /locus_tag="OO309_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="involved in fimbria production; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HCR5849233.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEIS"
BASE COUNT          141 a          106 c          137 g          144 t
ORIGIN      
        1 gtatcgaacc atcaatgact acggggcgga aacgccgcaa ctcaactgta aatcgtaagc
       61 cgtcttcagt taagagagcg agatgtatag aactcatcga caacacagcc tgttaagctc
      121 tggtggagtg ccatcgttta ttggtgggct ggtggtgttt gtgtcggcag cgttcaatgc
      181 acaagctgaa acctggttcg atcctgcttt tttcaaagat gatccctcaa tggtggccga
      241 tttgtctcgt ttcgaaaaag gacaaaaaat aacgccaggg gtttatcgtg tcgatattgt
      301 tctgaatcag acaattgtag atacgcgcaa cgtcaatttt gttgagataa cgccagagaa
      361 ggggattgcc gcctgtttga cgactgaaag cctggatgca atgggtgtta atactgatgc
      421 gtttccggct tttaaacaac tggacaaaca agtgtgtgtg ccattggcgg agattattcc
      481 ggatgccagc gtaacgttta atgtgaataa actccgtctg gaaatttc
//
LOCUS       DAJTQQ010000335          525 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000335 DAJTQQ010000000
VERSION     DAJTQQ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.348"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            2..508
                     /locus_tag="OO309_004377"
     CDS             2..508
                     /locus_tag="OO309_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR5849234.1"
                     /translation="MPVITTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGND
                     LKELRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRD
                     PSTPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDK
                     LVERLMQL"
BASE COUNT          141 a          116 c          128 g          140 t
ORIGIN      
        1 gattcctgtt attaccaccg aacttttggc gcagctttac ggcactgagc cggtgcgtat
       61 tcgccagaat catcatgaga acaaagtacg cttcgttgaa gggaaacact ttttcaaagt
      121 tgttggtaat gaccttaaag aattgcgggt cgctttaaac tactcacaaa atccagtttc
      181 acccaaagcc cgctccctca tcctctggac agaacgaggc gcagcacgcc acggcaaaat
      241 gctcgaaacc gatcaggcgt gggatgtgtt cgaaaaactg gaagactgct atttcagtca
      301 aagggatccg tcaacgccag tttcatgcca gaaaagttac gacacgcgag ttctctgtta
      361 tcagagaggc ggtgtcactg tttccacaat tcagttacgg gatgatgata ttgttatttc
      421 ccttgagtca tggctggaac tggcgagagc caatggttgg tttgttgttc gcagagataa
      481 actggtggaa aggttgatgc agctttaaaa aagttcttgc atttt
//
LOCUS       DAJTQQ010000336          511 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000336 DAJTQQ010000000
VERSION     DAJTQQ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.349"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            1..511
                     /locus_tag="OO309_004378"
                     /pseudo
     CDS             1..511
                     /locus_tag="OO309_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          127 a          128 c          141 g          115 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccagtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
      301 gagataatgg gccgggtcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
      361 acggaagtgg cctcccgtta aatatcgtgc taagccccgg acaagctcat gaaagccagt
      421 tcgcgcaacg acttctggac gggattggcg ttcagcgtca gaacggcagc atgaaacgcc
      481 gtggacatgc ggtactggct gataaagcgt a
//
LOCUS       DAJTQQ010000337          505 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000337 DAJTQQ010000000
VERSION     DAJTQQ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.350"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..333
                     /locus_tag="OO309_004379"
     CDS             <1..333
                     /locus_tag="OO309_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5849235.1"
                     /translation="GKVERMVQYTRNSFYIPLMTRLRPMGSTVDVETANRHGLRWLHD
                     VANQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGENLVSFDNPPQHHPLSIY
                     DSFCRGVA"
     gene            330..>505
                     /locus_tag="OO309_004380"
     CDS             330..>505
                     /locus_tag="OO309_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5849236.1"
                     /translation="MMMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFL
                     EHLLHEEKLARHQR"
BASE COUNT          133 a          140 c          133 g           99 t
ORIGIN      
        1 ggtaaggtgg aacggatggt gcagtacacc cgtaacagtt tttacatccc actaatgact
       61 cgcctgcgcc cgatggggag cactgtcgat gttgaaacag ccaaccgcca cggtctgcgc
      121 tggctgcacg atgtcgctaa ccaacgaaag catgaaacaa tccaggcccg tccctgtgat
      181 cgctggctcg aagagcagca gtccatgctg gcactgcctc cggagaaaaa agagtatgac
      241 gtgcatcctg gtgaaaatct ggtgagcttc gacaaccccc cccagcatca tccactctcc
      301 atctacgact cattctgcag aggagtggcg tgatgatgga actgcaacat caacgactga
      361 tggcgctcgc cgggcagttg caactggaaa gccttataag cgcagcgcct gcgctgtcac
      421 aacaggcagt agaccaggaa tggagttata tggacttcct ggagcatctg cttcatgaag
      481 aaaaactggc acgtcatcaa cgtaa
//
LOCUS       DAJTQQ010000338          499 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000338 DAJTQQ010000000
VERSION     DAJTQQ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.351"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            168..>499
                     /locus_tag="OO309_004381"
     CDS             168..>499
                     /locus_tag="OO309_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5849237.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTR"
BASE COUNT          155 a          114 c           96 g          134 t
ORIGIN      
        1 catcagatca gcgatttctt cacgcttcat tcagtatctc catacaatct attaatcatt
       61 taaatgatag gtctaatcat gatttagggt ctaatcaact taatgatcaa tatctatagt
      121 gatatcacca accgtcaaaa cgaaccgaca actccatgag gaactgaatg atgaataata
      181 aagtcagctt cactaacagc aataatccaa ccatctcttt gtctgcagtg atctatttcc
      241 ctccaaaatt tgatgaaacc cgtcagtatc cggcaattgt ggtatctcac ccaggtgggg
      301 gcgttaaaga gcaaacggcc ggaacctatg ccgaaaagct ggcggaaaag ggatttgtca
      361 ctattgctta tgacgcatct tatcaaggtg aaagtggcgg cgagccgcgt cagttagaaa
      421 acccatacat tcgtactgaa gacgtcagtg ccgtgattga ttacctgaca acgctttctt
      481 atgtcgacaa tacccgaat
//
LOCUS       DAJTQQ010000339          492 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000339 DAJTQQ010000000
VERSION     DAJTQQ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.352"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..261)
                     /locus_tag="OO309_004382"
     CDS             complement(<1..261)
                     /locus_tag="OO309_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5849238.1"
                     /translation="MEYRTWITEALRLHFEEHLPRVVAGRRLGVPKSTVCGMFVRFRN
                     AGLSWPLPAGMSEQELDALLYESASTVPVVLTESTVMPKLPVV"
     gene            complement(292..>492)
                     /locus_tag="OO309_004383"
     CDS             complement(292..>492)
                     /locus_tag="OO309_004383"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849239.1"
                     /translation="NFRIPFVINTYKPEDQFFVSGIHKERNIQVCATLNGIVKIIKAQ
                     NLSFFVTGSFKPSGLFPPAPQK"
BASE COUNT          128 a          103 c          138 g          123 t
ORIGIN      
        1 taccaccggg agtttgggca taacggtgct ttcagtaagt acgacaggta ctgtggaggc
       61 actttcgtaa agaagggcat caagttcctg ctccgacata cctgcgggca gaggccatga
      121 aaggccagcg ttgcggaagc gcacgaacat accacagaca gttgattttg gtacgcccag
      181 gcgacgtccg gctacaaccc gtggtaaatg ttcttcgaaa tgaaggcgta aagcttcagt
      241 gatccaggtc cggtattcca tacgatagtg tccacttcaa aatgatggac attatttttg
      301 tggagccgga ggaaacagac cagacggttt aaatgagccg gttacgaaga agctgaggtt
      361 ctgcgctttg ataattttga cgatgccgtt gagtgttgcg cagacctgaa tattccgttc
      421 tttgtggatg ccggaaacaa aaaactggtc ttctggtttg tacgtgttga tgacgaaggg
      481 tatccggaaa tt
//
LOCUS       DAJTQQ010000340          490 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000340 DAJTQQ010000000
VERSION     DAJTQQ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.353"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..221
                     /locus_tag="OO309_004384"
     CDS             <1..221
                     /locus_tag="OO309_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inhibitor of g-type lysozyme"
                     /protein_id="HCR5849240.1"
                     /translation="VHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLPASG
                     KYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(329..>490)
                     /locus_tag="OO309_004385"
     CDS             complement(329..>490)
                     /locus_tag="OO309_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849241.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          157 a          116 c           88 g          129 t
ORIGIN      
        1 aagtgcatgt gagtatctct aatgaaggcg ctgatactta tctcttcggg ccaggaatcg
       61 atgattctgt tgatttatcc agatattccc cagaactcga tagtcacgga caatactcac
      121 tccctgcttc tggcaaatat gagctgcgag tgctgcaaac tcgaaacgac gcccgtaaaa
      181 acaaaactaa aaaatataac gtcgatattc aaattaagta aatgtcaacc gacggggact
      241 atccccgtca tagacgttaa cgccccactc ctctctgtcg ctcaagaggt taatgacgaa
      301 tagcagagaa cataatgtaa acaaggcatc actcacgaca cataaataac atctttgtgg
      361 tggtttgatt gatttttcca ctccgcttac catctacacc aaaataacga tcatcacgac
      421 ataatttttc tttaacatcg caaaatacac cgttagcgaa tgtaaattcg ctgggatcgt
      481 agccttgtaa
//
LOCUS       DAJTQQ010000341          490 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000341 DAJTQQ010000000
VERSION     DAJTQQ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.354"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..233
                     /locus_tag="OO309_004386"
     CDS             <1..233
                     /locus_tag="OO309_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5849242.1"
                     /translation="NGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGRLKEY
                     RRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            348..>490
                     /locus_tag="OO309_004387"
     CDS             348..>490
                     /locus_tag="OO309_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR5849243.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVA"
BASE COUNT          146 a          123 c          122 g           99 t
ORIGIN      
        1 ataacggtat aaaggcagta atcccccgaa agtcaaatga gaaaatggca tcggatggac
       61 gtgcacagct tgatcgtgat gcgtactgca atcgtaatgt cgttgaacgg tgctttgggc
      121 ggctgaaaga atatcgccgc atcgccacgc gttacgacaa aacggcgaga aattacctgg
      181 cgatggtgaa actgggctgc atccgactct tttatcaacg cttacgtaat taagggacac
      241 agcctaaata gctgcgcgga atagtagatc cctttgaggg aacttagccc ggattgtgcg
      301 atctgatcaa tcgccaaatc aaaacaaatc accaaccgga ctgagcaatg ccgatcatag
      361 caccaatttc ccgtgacgaa cgacgcctga tgcagaaagc catccataaa acacacgata
      421 aaaattatgc ccgcagactg actgccatgc tgatgctgca ccggggcgac cgtgtcagcg
      481 acgttgccag
//
LOCUS       DAJTQQ010000342          488 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000342 DAJTQQ010000000
VERSION     DAJTQQ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.355"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
BASE COUNT          167 a           92 c          105 g          124 t
ORIGIN      
        1 ttaatcccgt actctctggc cagttgggcc acgcaggccc ctagctggta agatttttca
       61 ataagatcaa gcttaaactc catagagtca tagagtaaga ggcacgaatc ctgcgggggc
      121 gaagagaatc tgacattgat agagtccatt aacgttataa gaggactcta tcatcgccaa
      181 tcatcagaca ttgaaataga cgacactgag atgacgctta caattctttt ggtttctgta
      241 ataagtaatc tgacatcccc cgtgcccaga agaggagagg gatatcaagc ccaagggtta
      301 ataattgata ctacggttaa aattgccttc agtaaaggaa acgtgattaa attcagggga
      361 tttcagagtg aaaaccatac aacatattaa aatctgttca tttagctata atacatacaa
      421 gaaagtgtcc agttagataa tgttatctaa ataaccacag ataaaaacgc acataattgg
      481 gggggggg
//
LOCUS       DAJTQQ010000343          487 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000343 DAJTQQ010000000
VERSION     DAJTQQ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.356"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
BASE COUNT          159 a           99 c          105 g          124 t
ORIGIN      
        1 tcggagattg aagtccgcca gcgtcatgtc cagacgccat tgatgagaac atcatcccca
       61 cacctgacaa tccgatataa ataatacgca tcatattcat agcacatcct gataatccaa
      121 ccgtacaaca cttatgctca tttcgtcatc aatccgtaat caaaaccgct gacctggacg
      181 agttacgatg acctgacaga agtatcagac gtcttctgcg gtttctatga tagagcaatt
      241 ctgtggcgga tattaagata acgggtatct gttctttaat gatgctgcaa aaaatggaaa
      301 gttaagaaag cctggcgtgg tgaaaaaata cacttgcctc aatactggat atttaaacag
      361 gtctggagat gctgtcacga ttagggtgcg gaaaaaggaa gcgtaaataa gaagaatcaa
      421 aagtacgacg gcaatgggtt gattgacaag ggtatttttt aagctatgaa tcagcgccat
      481 ttatcac
//
LOCUS       DAJTQQ010000344          480 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000344 DAJTQQ010000000
VERSION     DAJTQQ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.357"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(198..344)
                     /locus_tag="OO309_004388"
                     /pseudo
     CDS             complement(198..344)
                     /locus_tag="OO309_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            398..>480
                     /locus_tag="OO309_004389"
     CDS             398..>480
                     /locus_tag="OO309_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HCR5849244.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLR"
BASE COUNT          141 a          109 c          122 g          108 t
ORIGIN      
        1 acgtaagaaa gtggcaggct catatgacgg acctctgtaa tgcggtatcc aacccgcgaa
       61 tatcagcatg attcaccgtc ggatttactg ctacgtcgcc ctcagggaat taaacctaaa
      121 aagaaaatcg tcaaaattag agattccggt ttgacacgga aagtcatatc agcgtcaacg
      181 gagcaccgta ttgacgctta tttattggtg agaactacgt tccatggcag gagttcgtca
      241 acacggttgg agggccattc aggcagtatg ctcagaatat ggcgcagata cgcttccgga
      301 tcgataccgt tcagacggca ggtgcctgct tttccggcaa caagcaggaa tctcagcgct
      361 gaagcaggaa ttagaggtaa aaacgcccta ccgggccatg aatcacccgg tcattggagt
      421 agtaaccaaa gcagatttag ccagcatgga acaaatatct ttggtgaaaa gttggttacg
//
LOCUS       DAJTQQ010000345          476 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000345 DAJTQQ010000000
VERSION     DAJTQQ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.358"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
BASE COUNT          107 a          126 c          151 g           92 t
ORIGIN      
        1 gggctggcgc ggttcctggc gacctgaaag cccctggtta ggggtatcgg cgcgcctgac
       61 ctcctgagag cctctgtaag cgtttttcgc gccttccatg ccctgacggg catttaaacc
      121 cgtttcgtgc gtctgagagc ttctgagcgt gtttgaaggg ctatctgacg actcatcccc
      181 gaaagaacaa caacgggatc accgacaaca tgcgagaggt gatcccgctc gccgcaagtc
      241 gatgaccgag cgtagcgagc gaatcgacga ggaagcggaa gagaaccgga agttacattg
      301 agcacttacg cacggatgcg gggtgtcggg gtgaagccct gaccaagtgg ggaatgtctg
      361 agtgcgcgtg cgcggtccga cattcccaca tcctgtcccg atttctgagg cgttttaacg
      421 gtgaacggac ggcaaaaaca tgatcgagcg tggagagcgc gaaatcgggc aaaatc
//
LOCUS       DAJTQQ010000346          474 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000346 DAJTQQ010000000
VERSION     DAJTQQ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.359"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(162..392)
                     /locus_tag="OO309_004390"
                     /pseudo
     CDS             complement(162..392)
                     /locus_tag="OO309_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT          150 a          120 c           90 g          114 t
ORIGIN      
        1 tattgaattt gcatggtctt gtaggccaga taagacgttc acgtcgcatc cggcatgaac
       61 aaagcgcact ttgtcaaaaa tctaacctac tttttaattc aggaaattac cgcaaagccc
      121 acgccatcat acccaacgta acaaaactca ggcacgttcc ccactcgccc cgagaaaaat
      181 agcattaatg cgcccagcgt cagcataaaa atattgagca gaaggtaaat tgttggcgta
      241 aagagtaaat tgcaaacctc aggaatagcg gcaagcccag cccccagcat ggcaataacc
      301 gctgcaaaaa attgcgtcag tattgggatg ccgaaaaaag tcattaccag cgaggtcaaa
      361 atccattttc tattttgcat tattctttcc attctttctg aatggcgaaa ttatactccc
      421 cgagtcccct tgccccttct ggacactttt ccgaaatgat ggcgaaaaaa acgg
//
LOCUS       DAJTQQ010000347          467 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000347 DAJTQQ010000000
VERSION     DAJTQQ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.360"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(218..>467)
                     /locus_tag="OO309_004391"
     CDS             complement(218..>467)
                     /locus_tag="OO309_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849245.1"
                     /translation="QQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFFH
                     GKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
BASE COUNT          126 a          136 c          105 g          100 t
ORIGIN      
        1 ggttgattga caccacagtg cgaattcggc gaatgcgaga tcacggtacg attccgcacc
       61 acactaccaa tcaatacccc gggttttatt caggtaagca ggactgcgga tatccggtgt
      121 cgcgcctttg tgtcacgaac ggggtgggtg cgaaacacca gataaaatac aggttggcaa
      181 tacctctgaa cgccctgagc aggtcgggca tttcggatta agtactcgca cctccgcagt
      241 cctcaaacaa gtctggctgg tagctgtaaa cagacttcgt acataccgct ctgcaataaa
      301 tccccgtacc acaggcctca cagaacttct tcccatgaaa aaactgctca cacatcacac
      361 aatgccactc cagcacgacc ggtaatggcg ataaaaacat cgccatatcc tcaatgtaag
      421 ggtgggactt ctccggattc agcaccacac agtccgcctt ctgttgc
//
LOCUS       DAJTQQ010000348          464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000348 DAJTQQ010000000
VERSION     DAJTQQ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.361"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(21..>464)
                     /gene="queE"
                     /locus_tag="OO309_004392"
     CDS             complement(21..>464)
                     /gene="queE"
                     /locus_tag="OO309_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR5849246.1"
                     /translation="AVIGRQGYTARHVVITGGEPCIHDLLPLTDLLEKNGFSCQIETS
                     GTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKHPVGRVRDIEALDELLATL
                     TDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHKYLNIA"
BASE COUNT          110 a          122 c          106 g          126 t
ORIGIN      
        1 taacgcttat aaatgtgtaa tcaggcaata tttaaatatt tatgtgtttg catcgacaaa
       61 cgccaattac gcgcaataca ggtttcaatg cacaatcgtg tggcatcatc cttttggcta
      121 atcggctgca gtgcaatgac tcgcggttta tcatcggtca gcgttgccag tagttcatcc
      181 agtgcttcaa tatcgcgtac gcgccccact ggatgcttga tttcgttggc tcgctccagt
      241 gcctgtgaca acacttcata gccgccgcgc atgttcagct ttggcgatac ggtaacccag
      301 gtattcggtg tgcagcgtac ttcatgagtt ccactggttt cgatctggca gctaaaaccg
      361 ttcttttcga gcaggtcagt cagtggcagc aaatcatgaa tgcaaggctc accacccgta
      421 atcaccacat gccgcgcggt atatccctga cgaccaatga cagc
//
LOCUS       DAJTQQ010000349          463 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000349 DAJTQQ010000000
VERSION     DAJTQQ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.362"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..>463
                     /locus_tag="OO309_004393"
     CDS             <1..>463
                     /locus_tag="OO309_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5849247.1"
                     /translation="YASAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWV
                     SEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGS
                     SLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEAGC"
BASE COUNT          117 a          109 c          142 g           95 t
ORIGIN      
        1 tatgccagtg cagggttact tgcacggatc ctggtcagca aatatatgga acatatccct
       61 ttatatcgcc agtcagaaat atacgcgcga cagggcgtgg agctgagccg taataccatg
      121 gtgcgctggg tatcagaaat ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac
      181 tatgttctgg aggcaggaaa ggtgcacgca gatgacactc cggtgaaagt actggccccg
      241 gggaacggaa agacgaaaac gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg
      301 ggttcatccc tgccggcagc cgtctggttc gcgtattcgg cagatcgcaa aggagaacat
      361 ccgcagctcc acctggcaaa gtatcagggc gtactgcagg ctgatgccta tgcaggttat
      421 aacgtactgt acgaaacggg ccgggtgaag gaagccgggt gcc
//
LOCUS       DAJTQQ010000350          459 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000350 DAJTQQ010000000
VERSION     DAJTQQ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.363"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
BASE COUNT          130 a           92 c           84 g          153 t
ORIGIN      
        1 aattcatcca gcagtttatc atttcccata atgtcactta ccctcatcgg taaaaaaatt
       61 gcctttcgac cattacgata cattattgat tttggggttt cagggaagta atccatttcg
      121 actataactg acaggtcatc acgacgtatg actgcgtatt tgctactaaa tagtttcttt
      181 attttttcca cgatgccccc aggtttataa gtacaaacgg ttatatccac atagagacaa
      241 aaatattaat ctgataaata tttatttcac gccgtatatt tgattgttta atgtgcaagt
      301 acaatgactt ttattttttg ttgtgtatat aatcaaatat atggttattt ttcaccctgc
      361 gtattcagca cgcaacaaaa aacccgccga agcgggttta gtgcgggtgc gttgaggatg
      421 cctgacacat gagaggtggc gagggatttc tcccccgcc
//
LOCUS       DAJTQQ010000351          457 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000351 DAJTQQ010000000
VERSION     DAJTQQ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.364"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..>457)
                     /locus_tag="OO309_004394"
     CDS             complement(<1..>457)
                     /locus_tag="OO309_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5849248.1"
                     /translation="RVWPTKAENHLRFNIINDKENRVNGIALYYRLPMVQVNDEQSFV
                     EQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQSLFFRVNARDDNMQD
                     AANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERDLRMLTS"
BASE COUNT          113 a          112 c          103 g          129 t
ORIGIN      
        1 actggtcagc atacgtaaat cacgttcagc ttgctgatca accgcatttt tcagccaggt
       61 gaggcgggta gatttgacat catcgagttc ttcagcagaa aaaccatgct gatcaatggt
      121 tgccaactct gccattaatg cattcgcagc atcctgcata ttatcgtctc gtgcatttac
      181 acggaaaaac agcgactgat aatcgggtgc aattttaacg ctgcgcgcag tgccgccaga
      241 aatagtcttc aactcgcccg actgtatgcg ttcctgcaga cgttgattga acagctgaac
      301 taacatgctc cattcagctt gttcgacaaa gctttgctca tcgttcactt gtaccattgg
      361 caggcgataa tagagtgcga tgccgttcac ccggttttct ttatcattaa tgatattaaa
      421 gcgcaggtgg ttttcggctt ttgtcggcca gacgcga
//
LOCUS       DAJTQQ010000352          451 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000352 DAJTQQ010000000
VERSION     DAJTQQ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.365"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..108
                     /gene="relB"
                     /locus_tag="OO309_004395"
     CDS             <1..108
                     /gene="relB"
                     /locus_tag="OO309_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCR5849249.1"
                     /translation="PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            108..395
                     /gene="relE"
                     /locus_tag="OO309_004396"
     CDS             108..395
                     /gene="relE"
                     /locus_tag="OO309_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCR5849250.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
BASE COUNT          122 a           90 c          123 g          116 t
ORIGIN      
        1 ccgttcaaac agacactcct gagtgatgaa gatgctgaac ttgtggagat agtgaaagaa
       61 cggcttcgta atcctaagcc agtacgtgtg acgctggatg aactctgatg gcgtattttc
      121 tggattttga cgagcgggca ctaaaggaat ggcgaaagct gggctcgacg gtacgtgaac
      181 agttgaaaaa gaagctggtt gaagtacttg agtcaccccg gattgaagca aacaagctcc
      241 gtggtatgcc tgattgttac aagattaagc tccggtcttc aggctatcgc cttgtatacc
      301 aggttataga cgagaaagtt gtcgttttcg tgatttctgt tgggaaaaga gaacgctcgg
      361 aagtatatag cgaggcggtc aaacgcattc tctgaaccaa agcatgacat ctctgtttcg
      421 caccgaaggt gacacttctg ctttgcgttg a
//
LOCUS       DAJTQQ010000353          446 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000353 DAJTQQ010000000
VERSION     DAJTQQ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.366"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..>446
                     /locus_tag="OO309_004397"
     CDS             <1..>446
                     /locus_tag="OO309_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5849251.1"
                     /translation="VQVFAKATGKTEQELFDAMKKGALKSADTLQKVTQELNKQITAK
                     GGWKAISESTQAQLGNLKNSWNTTLDSIFRGSENGLQDFTRSLTNLLNALGGSGKSLG
                     ESLGSLMTSMSHGVDSLTDISYRVRAFFDEVTLAYRPLNDTQRKIV"
BASE COUNT          140 a           79 c          104 g          123 t
ORIGIN      
        1 gttcaggtat tcgctaaagc aaccggaaag actgaacagg aattatttga tgctatgaag
       61 aagggggctt taaaatctgc cgatactctt cagaaggtca ctcaggaact gaataaacaa
      121 atcaccgcta aaggtggctg gaaagctatt tcagaaagta cacaggcaca actagggaac
      181 ttgaaaaact cttggaatac cacgcttgat agtatcttca gaggttctga aaatggtctg
      241 caagatttta caagaagctt aaccaatctt ttaaacgctc ttggtggaag tggtaagagt
      301 cttggtgaat ctcttggtag tctcatgact tctatgtcac atggtgtcga ttcattgacc
      361 gatattagtt acagggtaag ggcgttcttt gatgaggtga cattagctta ccgtccacta
      421 aatgacactc agaggaaaat tgttga
//
LOCUS       DAJTQQ010000354          444 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000354 DAJTQQ010000000
VERSION     DAJTQQ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.367"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(60..383)
                     /locus_tag="OO309_004398"
     CDS             complement(60..383)
                     /locus_tag="OO309_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCR5849252.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
BASE COUNT           96 a          129 c           98 g          121 t
ORIGIN      
        1 cactttggga gcttaccgtg ttcataccgt ctgttttcaa cagttttctc cccgggaagc
       61 taatctgcca taagcctgga taacagggca cggtgatact ccgtaatagc gatcagcacc
      121 tccgcatact ccctgtcccc gatacgcttg ccgtcgataa agagtttttt ctgtaacgcc
      181 cccagtctca taacttcttt cagtacccga tcatcagtca gtgagttaac gttcttaccg
      241 agagctgccg cccgtaaaaa accagaaacg gtcttgccgc attcagcagc cctttttctg
      301 atttcctggt cttcttctgc cgtcagacga acagggcgac tgatagcccg cctgcgcgta
      361 ttacttccac ttcgttttgt cataaaacat ggctccgtat ctgacacggg gtgtcggggc
      421 gaagccctga ccagggtaat tgta
//
LOCUS       DAJTQQ010000355          442 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000355 DAJTQQ010000000
VERSION     DAJTQQ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.368"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..>442)
                     /locus_tag="OO309_004399"
     CDS             complement(<1..>442)
                     /locus_tag="OO309_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR5849253.1"
                     /translation="DPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTAFFEAYMDADYTD
                     RGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARSM
                     DVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRA"
BASE COUNT          103 a          114 c          122 g          103 t
ORIGIN      
        1 ggcgcgggaa taatccatcc cgccaggcat ggtgatgaat ttgtcgcgga aaacagaaaa
       61 gggcggttta ttttcagcga taatttcctc gacacgttta gcgtgtgccg gatgaaggtt
      121 ataaatgtcc acgtccattg aacgagccag tacgccagtg gctacgtcgc gcgccagtga
      181 cgtcagatcg tgaacgaaac cttcgccgcg atcggtgagg ttcccgccgc cagcattagc
      241 gccggaagcc gtgcgcgtga tgcgtgaaac acgatttcct ttcatccact cttttgtcag
      301 cagaccgcga tcggtgtagt ctgcgtccat gtatgcttcg aaaaaagcag tcatcagccc
      361 caggcttgaa ttgcctggat tagggaaaac tctgtcagta tcacgcacca gtttgtggag
      421 atcgcgaatt tccagcggat cg
//
LOCUS       DAJTQQ010000356          439 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000356 DAJTQQ010000000
VERSION     DAJTQQ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.369"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..>439
                     /gene="intS"
                     /locus_tag="OO309_004400"
     CDS             <1..>439
                     /gene="intS"
                     /locus_tag="OO309_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR5849254.1"
                     /translation="EAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKIDGKEKILT
                     VGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKSSSNNNSFSAIYKEWYEHKKQVWS
                     VGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
BASE COUNT          137 a           90 c          114 g           98 t
ORIGIN      
        1 gaagcagcaa agccgaaaga aaaaccatac cgccttctcg atggtaatgg cctgtacctt
       61 tatgtccctg tatccgggaa aaaggtatgg cagcttcgct acaagattga cggtaaggag
      121 aaaatcctga ccgtcggaaa atatccgctt atgactttgc aggaggcaag ggataaagca
      181 tggactgcga ggaaagacat ctcggctggc atcgatccgg taaaggcgaa aaagtcttcg
      241 tctaacaaca attcatttag tgcgatttac aaggaatggt acgaacacaa gaagcaagtc
      301 tggtcagtag ggtatgcgac tgaacttgca aaaatgtttg atgacgacat tttacccatc
      361 atcggcggcc ttgagattca ggatattgag ccgatgcaac tgctggaagt aatccgcaga
      421 tttgaagatc gcggtgcaa
//
LOCUS       DAJTQQ010000357          434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000357 DAJTQQ010000000
VERSION     DAJTQQ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.370"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            35..434
                     /locus_tag="OO309_004401"
                     /pseudo
     CDS             35..434
                     /locus_tag="OO309_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAJTQQ010000358          431 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000358 DAJTQQ010000000
VERSION     DAJTQQ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.371"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..>431
                     /locus_tag="OO309_004402"
     CDS             <1..>431
                     /locus_tag="OO309_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5849255.1"
                     /translation="NRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPT
                     VRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEM
                     VRRYAHLAPNHLTEHARQIDLILNPSVPNLSQSRNKEGTND"
BASE COUNT          130 a           96 c          112 g           93 t
ORIGIN      
        1 aaaccgcgca attggcgttg cgctgaatga tactgcatgt cgcgtattga aaaaacaaat
       61 cggaaatcat caccgttggg tatttgtgta caaggaaagc tgtaccaaac cagacggaac
      121 gaaagcgcca acagtaagga agatgcggta tgacgcaaac acagcctgga aagcggcgct
      181 gagacgggct ggtattgatg atttcagatt tcacgacttg agacacacct gggcaagttg
      241 gctggttcaa gccggagtcc cgttatcagt gttacaggaa atgggaggct gggagtctat
      301 cgaaatggtt cgtcgatatg ctcacctcgc acctaatcac cttaccgaac acgcacggca
      361 aatagacttg attctgaacc catcggtccc aaatttgtcc cagtcaagaa ataaggaagg
      421 tactaatgat g
//
LOCUS       DAJTQQ010000359          424 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000359 DAJTQQ010000000
VERSION     DAJTQQ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.372"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            49..424
                     /locus_tag="OO309_004403"
                     /pseudo
     CDS             49..424
                     /locus_tag="OO309_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          112 a           89 c          106 g          117 t
ORIGIN      
        1 ccatcttaaa tgactctgag gtgtctgtta aacccgtggc gattcagtga gcgcattctg
       61 accgaccgtg aaaatggtga aaaaggcgat aattctcaag atacctctta tgcccgtgta
      121 ggcgtgaaag gtgaaactca gatcaaccct gaaatgactg gttacggtca gtttgaacta
      181 gacctggaag catcaaacag acacaaccct gaccaaactc gtttggcata tgctggtttg
      241 agttacaaag attttggttc attcgactac agtcgtaatg tcggtgtcgc gtatgatgca
      301 gaagcattta ctgatatgtt tgttgagtgg ggcggtgact catgggctgg tactgacctg
      361 ttcatgacca accgtaccaa cggtgttgca acctatcgta acacagattt ctttggcatg
      421 gttg
//
LOCUS       DAJTQQ010000360          422 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000360 DAJTQQ010000000
VERSION     DAJTQQ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.373"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            70..>422
                     /locus_tag="OO309_004404"
     CDS             70..>422
                     /locus_tag="OO309_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5849256.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYAL"
BASE COUNT          136 a           78 c          102 g          106 t
ORIGIN      
        1 cacagatgtg tcatgatggc ttagatttga accaacgtta gtgcatggga ttttcagagg
       61 gaaaaaatca tgtttattga ttcagaaaaa cgactgaaac aactttcaga tgaggcaaag
      121 aaaaacaccg aagatctcga agaagcaaag aagaattcaa ggtttacaca ggtatcccca
      181 aaaggttggg aacgcgttcg agagctgctg aaggatagcc aaggcatatc agcactgaag
      241 ctgtactcat ttttagcgga gcatatcgat cctacgtgtg gcgctgtcgt tgcggatcag
      301 caattcctag ctgaaaaact tggagttagc agaagcacaa ttattcggtg gctcaattac
      361 ttagaatcaa aaaatgcatt agttagaatc cccgttgctg gtaaggtttg tgcgtatgcc
      421 ct
//
LOCUS       DAJTQQ010000361          420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000361 DAJTQQ010000000
VERSION     DAJTQQ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.374"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(100..>420)
                     /locus_tag="OO309_004405"
     CDS             complement(100..>420)
                     /locus_tag="OO309_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5849257.1"
                     /translation="LIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTV
                     LSLAIDEHELANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVF
                     PLFC"
BASE COUNT          163 a           87 c           85 g           85 t
ORIGIN      
        1 gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta ctgagagaaa
       61 aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca atgggaaaac
      121 aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac cgttttcacg
      181 aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc aaaaggtcat
      241 tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa cagttaacag
      301 cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca cgagcaaaac
      361 gctcccggct gcaaccagat tgggtttttg atgctgtaaa atgagtgcca aaaaaataag
//
LOCUS       DAJTQQ010000362          417 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000362 DAJTQQ010000000
VERSION     DAJTQQ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.375"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..354)
                     /locus_tag="OO309_004406"
     CDS             complement(<1..354)
                     /locus_tag="OO309_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR5849258.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQR"
BASE COUNT          111 a          126 c           84 g           96 t
ORIGIN      
        1 acgctgctct tcatcaatga atcccatgcc tttacgaacg gattcaatct ggtctggata
       61 ttgagtagcc aactgacgca aagcaccgcg atcaccagac gcataagcat tagcgtacgc
      121 ctgctgaaat tctttctgcc gctgagcctg cttttcctgc tgaaaaacac ccgcaatacc
      181 tgaaagacct tgcaaagcag tcagcccaac attgttagcg cctgaacgct caatatcatt
      241 gttctgcctg ataagctgaa gcgtattgcc gatgtcattt acgctcggag cgtttgagtt
      301 gacgccgccg ataccagcca acaatccgcc gtttgttcct tgccaagtag ccatgattcc
      361 cccttaaaac aacgagccaa gcaatccgat accagcacca ataccagcgc cccaagg
//
LOCUS       DAJTQQ010000363          415 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000363 DAJTQQ010000000
VERSION     DAJTQQ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.376"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..390
                     /locus_tag="OO309_004407"
     CDS             <1..390
                     /locus_tag="OO309_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR5849259.1"
                     /translation="QQAQQQPQQPSAEQIQAQGILLQGQAELLKAENQQAQIQVEAAK
                     VEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRSKDARANAELLLKDADQ
                     THSQRMDFANLMRQVQIPSGGVAETPQ"
BASE COUNT          131 a          112 c          100 g           72 t
ORIGIN      
        1 cagcaggcac aacaacagcc gcagcagcca tcagcagagc aaattcaggc gcagggtatc
       61 cttctgcaag gtcaggctga attgctcaag gcagagaacc aacaggcgca gattcaggtt
      121 gaagctgcca aggttgaagc ccaaaaccaa ctcaacgccg cgaagattgc agaaatattc
      181 aacaatatgg acctcgacaa gcaggcagaa ctgcgtgagt acctcaggct cgtaggtcaa
      241 ttccagcaac agcgcagcaa agatgctcgt gctaacgctg agctgcttct taaagatgca
      301 gaccagactc attcacaacg catggatttc gcgaatctta tgcgtcaagt tcaaatcccc
      361 tccggcggag tagccgagac acctcaataa gagagagtta atcatggacc aaacc
//
LOCUS       DAJTQQ010000364          396 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000364 DAJTQQ010000000
VERSION     DAJTQQ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.377"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..>396)
                     /locus_tag="OO309_004408"
     CDS             complement(<1..>396)
                     /locus_tag="OO309_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849260.1"
                     /translation="ITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKFSNVEPYMIERMK
                     AELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFIPRVFFSANKSTR
                     IGMLCGLLETDGYVEKDGTLVFSSASEELR"
BASE COUNT          103 a          110 c           82 g          101 t
ORIGIN      
        1 gcgcagttct tcacttgctg aggaaaaaac aagcgttcca tccttctcga catacccatc
       61 cgtttcaagc agaccacaca gcatgcctat acgcgttgat ttatttgcgg aaaaaaacac
      121 acgagggatg aatttgtttt ttgctgtgca ccccattagt ccataacctc gcagggtctc
      181 catgagtgga tttacctgac cacgcgccgt tgatatcagc cagtccttac catctccaga
      241 gaagttgtaa tcaggcagtt cagccttcat acgctcgatc atgtatggct cgacgtttga
      301 aaacttcacg ctcccattgc taagactacc atccccgaga agggagccga taacccatgc
      361 taatggctct gagtggtcgc caaagtcacc agttat
//
LOCUS       DAJTQQ010000365          392 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000365 DAJTQQ010000000
VERSION     DAJTQQ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.378"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..>392)
                     /locus_tag="OO309_004409"
     CDS             complement(<1..>392)
                     /locus_tag="OO309_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HCR5849261.1"
                     /translation="AEHAPIQSDPHELSAVVRVALGRAITQQWFPGKDEVHVIGLDTP
                     LERLLLQALQGGGGLEPGLADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLR
                     RSLPQLVVLSNLELSDNRHIRMTATIGG"
BASE COUNT           86 a          123 c          104 g           79 t
ORIGIN      
        1 taccgccaat tgtcgccgtc atgcggatat gtcggttatc agacagttcc agattcgaca
       61 ggaccactaa ctgcggcaag ctgcggcgca ggaagcgaga caataatggt cgcagcgcgt
      121 ggttcaccaa caatactggc ggcgcaccca gcatctcctg acgggataga gcttcctgag
      181 tttgcgccag taaacgatcc gccagccctg gctccagtcc tcccccgccc tgcagcgcct
      241 gtagtaacaa acgttccagc ggtgtatcga ggccaataac atggacttca tctttgccag
      301 gaaaccactg ctgggtaatc gcccgtccca acgccacgcg cacgacggcg cttaattcat
      361 gtggatcgct ttggatgggc gcatgttccg cc
//
LOCUS       DAJTQQ010000366          372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000366 DAJTQQ010000000
VERSION     DAJTQQ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.380"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..311)
                     /locus_tag="OO309_004410"
     CDS             complement(<1..311)
                     /locus_tag="OO309_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5849262.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAJTQQ010000367          371 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000367 DAJTQQ010000000
VERSION     DAJTQQ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.381"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..>371)
                     /locus_tag="OO309_004411"
     CDS             complement(<1..>371)
                     /locus_tag="OO309_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCR5849263.1"
                     /translation="LSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELK
                     PGAAPDDVHHQDNAQETKELAGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTY
                     AAGDLKYLHHATREQRKHITA"
BASE COUNT           78 a           86 c           99 g          108 t
ORIGIN      
        1 agcagtaatg tgtttgcgtt gttcacgggt ggcatgatgc aggtatttca gatccccggc
       61 agcatacgta aaaatgtgca gaagacgctg tgtgaaccgc aaagtggcta cagagacttc
      121 gcaatcctgg caatcctcgt gggcgtctgc ctgcgcgttt tcttcctggc ctcccgccag
      181 ttctttggtt tcttgagcat tatcctggtg gtgaacgtcg tctggcgctg ctcccggttt
      241 tagttcccat gtcatggagt ctttgctgag ttgatagcgt tcactccagg taaaatcgat
      301 ctcaccttca gggggaaggt cattaacgac aggaaaattt gtggcaacag ctttaaaata
      361 gttgctcagt t
//
LOCUS       DAJTQQ010000368          367 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000368 DAJTQQ010000000
VERSION     DAJTQQ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.383"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..>367
                     /locus_tag="OO309_004412"
     CDS             <1..>367
                     /locus_tag="OO309_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849264.1"
                     /translation="LKIKEPRNKESLFFMSVMELRDLLIALESRLHQKHKLDAAARLQ
                     YEQARDKVIKKMAENIPEILVDELKNADINRRVNTLELTDNQGENLTFVLTLHDGSKC
                     ELVVNELQIEMLARAIIHAI"
BASE COUNT          117 a           73 c           82 g           95 t
ORIGIN      
        1 ctgaaaatta aagaacctcg caataaagaa tcgttgttct ttatgtctgt catggaactg
       61 cgcgacctgc tgattgcgct ggaaagtcgt ctgcatcaaa agcataaact ggatgctgct
      121 gcacgccttc agtatgagca agctcgcgat aaagtcatta agaaaatggc agaaaacatc
      181 ccagaaattc tggttgatga gcttaaaaat gccgatatta atcgtcgggt taacacgctg
      241 gaacttaccg ataatcaagg cgaaaatctt accttcgtat taacgctaca tgacggtagc
      301 aaatgtgagt tagtcgtcaa tgagttgcag attgaaatgc tggcgcgagc aatcattcat
      361 gcaatta
//
LOCUS       DAJTQQ010000369          365 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000369 DAJTQQ010000000
VERSION     DAJTQQ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.384"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
BASE COUNT          100 a           86 c          112 g           67 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggac
       61 caggccgcag gggtgaaact ctgcggcctt tttcgtaaga aaaatgcaac gattgcacgc
      121 ggataatatt ctgcggaatg cgcagcacag agtgaagacg tgacggattc cggaacagaa
      181 aaacagtcgt agaatcagcg gcaagccgga aaggttccgg tgaggcgcaa tgttgcgggg
      241 gctttatccc tggtggcatt ggttgctgga gagagaaaac ccccgcacgt tgcaggtatg
      301 cacctgacaa catcacgggg gctaatcttg aacccaaaca actcaagaat agccgcgaaa
      361 cgttg
//
LOCUS       DAJTQQ010000370          358 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000370 DAJTQQ010000000
VERSION     DAJTQQ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.385"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..233
                     /locus_tag="OO309_004413"
     CDS             <1..233
                     /locus_tag="OO309_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005160866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HCR5849265.1"
                     /translation="TKYLFFESQGIPDKVDIVSRLKTYGYSISGVETDDGYKALVRAF
                     QLHFRQKNYDGIMDAETAAILYALLEKYFPGK"
BASE COUNT           87 a           77 c           87 g          107 t
ORIGIN      
        1 tgactaaata tttatttttt gaatcgcagg gcattccgga taaggttgat atcgtgtcac
       61 ggctgaagac ctatggatac agtatttctg gtgtggagac ggatgacgga tataaggcgc
      121 ttgtccgcgc ttttcagctt catttccggc agaaaaatta tgacggcata atggatgctg
      181 aaaccgctgc catcctgtat gcattgctgg agaagtattt cccgggtaaa taaggagtga
      241 gtcgtcagag ctgcgatata tccccttcct ccatgggctg tctcttatac acaaatcccc
      301 acccggggat ttgtgatgtc agatgtcccc tgtaaaacta tgttttcatt gcgctcta
//
LOCUS       DAJTQQ010000371          354 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000371 DAJTQQ010000000
VERSION     DAJTQQ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.386"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            116..>354
                     /locus_tag="OO309_004414"
     CDS             116..>354
                     /locus_tag="OO309_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5849266.1"
                     /translation="MATVTVHCPRCHSDEVYRHGRSCSRHERFRCRSCKRVFQLTYSY
                     EARKPGVKEQIVEMAHNGAGGRDTARTLKIGINTV"
BASE COUNT           93 a           80 c           84 g           97 t
ORIGIN      
        1 aattacagga ctggcgaaaa gaacctcgta aaaagtattc gaatgaattc aaacttcggg
       61 tgatgtcttc aatcgacaga agtttctggt aatctactgg tatttaaggt tttccatggc
      121 taccgttact gttcattgtc cgcgctgtca ttccgatgaa gtttatcgac atggccgtag
      181 ttgttcccgg catgagcgtt tccgttgtcg ctcatgtaaa cgtgttttcc agctcactta
      241 ctcttatgaa gccagaaaac ccggcgttaa ggagcagatc gttgaaatgg cacataacgg
      301 cgcagggggc cgtgataccg ccaggacact gaaaatcggc atcaataccg tcat
//
LOCUS       DAJTQQ010000372          349 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000372 DAJTQQ010000000
VERSION     DAJTQQ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.387"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..269)
                     /locus_tag="OO309_004415"
     CDS             complement(<1..269)
                     /locus_tag="OO309_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HCR5849267.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALG"
BASE COUNT           86 a           75 c           85 g          103 t
ORIGIN      
        1 tctcctagcg ctagcgcaat aggctcatgc tgccagtgga tcatatcgtc actggtggca
       61 tgtccccagt gcattggtcc ccagtgttcg ctcatcgggt gatgttgata aaacgcgtga
      121 taacgatcgt taaaccagat caggccgttt ggatcgttca tccatccggc aggaggcgcg
      181 aggtgaaaat ggggatagaa agtgttaccc cggtgctcat gaagttttgc tagtgcgttt
      241 tgcgccgcat gcaatcgaga ttgcgtcatt ttaatcatcc tggttaagca aatttggtga
      301 attattaacg ttaactttta taaaaatata gtcccttact ttcataaat
//
LOCUS       DAJTQQ010000373          344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000373 DAJTQQ010000000
VERSION     DAJTQQ010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.388"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..>344
                     /gene="tuf"
                     /locus_tag="OO309_004416"
     CDS             <1..>344
                     /gene="tuf"
                     /locus_tag="OO309_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5849268.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DAJTQQ010000374          342 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000374 DAJTQQ010000000
VERSION     DAJTQQ010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.389"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..>342
                     /locus_tag="OO309_004417"
     CDS             <1..>342
                     /locus_tag="OO309_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849269.1"
                     /translation="RIDTKTAKKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQEE
                     LCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEAAK
                     RNVGTGANQIP"
BASE COUNT           88 a           79 c          107 g           68 t
ORIGIN      
        1 atcgtataga cacgaaaacc gcgaagaagg ataagttcgg cgcgggtaag aacggtttta
       61 cccgtggtaa cccccagact ggcacgcctg ccaccgatct ggatgatgac tactttgaca
      121 tgttgcagga ggagctttgc agcgtggtgg aggcatccgg tgccagcctg gagaaggggc
      181 ggcacgatca gttgcttacc gcacttcgcg cgctgctgtt aagccgcaag aatccgtttg
      241 gcgatatcaa atcggatggc acggtgaaaa cggctctcga aaaccttggt ttgggagaag
      301 cagctaaaag gaatgtaggt acaggggcga atcagatacc tg
//
LOCUS       DAJTQQ010000375          342 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000375 DAJTQQ010000000
VERSION     DAJTQQ010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.390"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..>342)
                     /locus_tag="OO309_004418"
     CDS             complement(<1..>342)
                     /locus_tag="OO309_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849270.1"
                     /translation="GFLFAHREECTVEIKKIINPRYTESGAVDCDVFFDDRDQAVPYT
                     ATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAWRAEQEAKLF
                     TFEWNGRIWNAG"
BASE COUNT           69 a          100 c           78 g           95 t
ORIGIN      
        1 ccagcattcc agatacgacc gttccattca aacgtgaaca gcttcgcctc ctgttctgcg
       61 cgccatgctt caatttcctg acgtctggcc tctctggccg cttccagcat ttctggtgtc
      121 acagtgaatg gggctatctc accccatttg ccgctttgca gttcctgcca gatttgctga
      181 cccgtcggag cgacatcatc agcggtggct gtgtagggga ctgcctggtc cctgtcgtca
      241 aaaaaaacgt cacagtctac tgcgccactt tcggtataac ggggattaat gattttttta
      301 atttccacgg tgcattcctc acgatgtgcg aataaaaagc cc
//
LOCUS       DAJTQQ010000376          342 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000376 DAJTQQ010000000
VERSION     DAJTQQ010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.391"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..>342)
                     /locus_tag="OO309_004419"
     CDS             complement(<1..>342)
                     /locus_tag="OO309_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5849271.1"
                     /translation="QKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPE
                     DVIRFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRY
                     RAKSLTFYGING"
BASE COUNT           76 a           97 c           89 g           80 t
ORIGIN      
        1 gccgttaatg ccgtaaaaag taagggattt ggctctgtac cgcggttctg ttgtctcagg
       61 gcgtgcatca gtccaccgga tgctgagcac cccttcaaac cgtgtgtcgg gtatgatgat
      121 gccgtagggg ccagcaacgg aatattcacc tggcagcgca ttccttaccc aggccaggaa
      181 atcactctta gtgtcaaaac ggataacatc ttcaggcaga aaagcacacc caaagccgaa
      241 tgcgccgggt atcgccagac ggccttttgt ccggtcgtaa atgtcgctct gtgcttccat
      301 cgtggccgca cttttcagcc ccagattatc ccgggacttc tg
//
LOCUS       DAJTQQ010000377          334 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000377 DAJTQQ010000000
VERSION     DAJTQQ010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.392"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..>334)
                     /locus_tag="OO309_004420"
     CDS             complement(<1..>334)
                     /locus_tag="OO309_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR5849272.1"
                     /translation="LKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWR
                     HFRQHLAVRWRKDCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNY
                     QKLDQRRI"
BASE COUNT          107 a           88 c           71 g           68 t
ORIGIN      
        1 ttgattcgac gttgatcaag tttctgataa ttccaacgac ggtcgacggg caccatccag
       61 tcaatcaaca tacctaccgc cagccctgtg atgacatcaa taaaatgatg ctgccatgtc
      121 gtcagcgtcg aaatggcgat gagtaaaaac catccgccac agtctttacg ccacctcaca
      181 gccagatgct gacgaaagtg acgccaaagt agccagcaga gaataatatg cagcgaagga
      241 gactggttat aaggcagatc aaacagttca agttgcgaaa atagccatcc tgtcacccca
      301 ctcacttcag gacggataaa actaaacttc agcg
//
LOCUS       DAJTQQ010000378          329 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000378 DAJTQQ010000000
VERSION     DAJTQQ010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.393"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
BASE COUNT           82 a           67 c           86 g           94 t
ORIGIN      
        1 aagcggactg acacatttct ctttgttctc attcagaaaa tctcatcagt cggcgttcag
       61 accggtgttg tgctttatgg atacgttacg ctcctaatga cgtcatttga cgttcaacag
      121 catcacgggg ccgcacgaca tttcacgtca gttagtgcta tagctcagga acaaattttc
      181 ccaaattggg atatgctcgc aaattgctgg tgatgtgggg agaatctggt tgagttcggt
      241 agaattgatt tggaggcaga acgcttaaat cgtggcgtcc tgaaacgaaa aacggacctc
      301 cgtggaggtc cgttgatatg aatttggtg
//
LOCUS       DAJTQQ010000379          324 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000379 DAJTQQ010000000
VERSION     DAJTQQ010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.394"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..>324
                     /locus_tag="OO309_004421"
     CDS             <1..>324
                     /locus_tag="OO309_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR5849273.1"
                     /translation="RISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKRLDKC
                     VFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRPVPEL
                     PEKYR"
BASE COUNT           88 a           74 c           92 g           70 t
ORIGIN      
        1 agcgtatctc tcgtgaaaaa ctgacgatta aaaaaatgat cgatctttat caagcgaaat
       61 gcccgcaggc gtcagcggag ccggagcatt acgaggcgtt gtttgtttac gcgcaaaagc
      121 ggctggataa atgtgtgttc ggcgaagaga aaccagcctg taagcagtgt ccggtgcact
      181 gttaccagcc tgcaaagcgt gaagagatga aacagattat gcgttgggcc gggccgagaa
      241 tgctgtggcg tcatccgatc ttaaccgtgc gtcatctcat cgatgacaag cgtccggtgc
      301 cagaactgcc ggaaaaatat cgtc
//
LOCUS       DAJTQQ010000380          323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000380 DAJTQQ010000000
VERSION     DAJTQQ010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.395"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..>323
                     /locus_tag="OO309_004422"
     CDS             <1..>323
                     /locus_tag="OO309_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR5849274.1"
                     /translation="RQEIEAWRTEQEAKPFTFEWNGRTWNADASSVARLSPVVMLAKS
                     VAAQTHMVWSDADNQQVKLSMPELEELAAAMVQAQVDRNDEIYRRQREMKEELSSLDD
                     LASIRA"
BASE COUNT           80 a           74 c          109 g           60 t
ORIGIN      
        1 cgtcaggaaa ttgaagcatg gcgcacagaa caggaggcga agccgttcac gtttgaatgg
       61 aacggccgca cgtggaacgc tgacgcctcg tcagtggccc gcctgtcccc ggtggtcatg
      121 ctggcaaaat ctgtcgcggc acaaacacat atggtgtgga gtgatgcgga taatcagcag
      181 gtgaaactgt cgatgccgga actggaagaa ctggcggcag caatggtgca ggcgcaggtc
      241 gatcgcaacg atgagattta tcgccgtcag cgggagatga aagaggagct gagcagtctg
      301 gatgatttgg cttcaattcg ggc
//
LOCUS       DAJTQQ010000381          322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000381 DAJTQQ010000000
VERSION     DAJTQQ010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.396"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            25..>322
                     /gene="hslJ"
                     /locus_tag="OO309_004423"
     CDS             25..>322
                     /gene="hslJ"
                     /locus_tag="OO309_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR5849275.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLN"
BASE COUNT           98 a           68 c           85 g           71 t
ORIGIN      
        1 cagaaactgt aattgagaac cacaatgaag aaagtagccg cgtttgttgc gctaagcctg
       61 ctgatggcgg gatgtgtaag taatgacaaa attgctgtta cgccagaaca gctacagcat
      121 catcgctttg tgctggaaag cgtaaacggt aagcccgtga ccagcgataa aaatccgcca
      181 gaaatcagct ttggtgaaaa aatgatgatt tccggcagca tgtgtaaccg ctttagcggt
      241 gaaggcaaac tgtctaatgg tgaactgaca gccaaagggc tggcaatgac ccgtatgatg
      301 tgcgctaacc cgcagcttaa tg
//
LOCUS       DAJTQQ010000382          317 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000382 DAJTQQ010000000
VERSION     DAJTQQ010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.397"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..>317)
                     /locus_tag="OO309_004424"
     CDS             complement(<1..>317)
                     /locus_tag="OO309_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR5849276.1"
                     /translation="ARTEATNDLYFSRVSQWDDWLSNYTTLQYRGQFDVVRPVVRKLV
                     AEMRRNPIDVLFRPKDGANPDAADVLMGMYRTDMRHNTAKIAVNVGVREQIESGVGAW
                     RLVT"
BASE COUNT           74 a           90 c           79 g           74 t
ORIGIN      
        1 gtgaccagac gccatgcacc aacgccggac tctatctgct cacgaacgcc aacgttaacg
       61 gcaatctttg ccgtgttatg gcgcatatca gtacgataca ttcccatcaa cacatcggca
      121 gcatcaggat tagcgccgtc tttgggtcgg aagagaacgt cgatagggtt ccggcgcatc
      181 tctgcgacca gtttcctgac caccgggcga acaacatcga attgtccgcg atattgcagg
      241 gtagtgtagt ttgatagcca gtcatcccat tgcgacactc ggctaaaata caggtcattt
      301 gtcgcctcgg ttctggc
//
LOCUS       DAJTQQ010000383          315 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000383 DAJTQQ010000000
VERSION     DAJTQQ010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.398"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
BASE COUNT           75 a           65 c           99 g           76 t
ORIGIN      
        1 caacacaaag gcaactaata aagatacaga taaagatcta aaaacagatc taacccatcc
       61 caaacccttc ccttccggaa gggagtttcg ggattttgtg gctggagtgc ttgaggggag
      121 attatctggc ggtactgcag cggaattttg taattctgcg gtggttgcgt tgcaggctgc
      181 tggcctggat gtctgtcgtg agtatccggt gccagagcgt ggtgacggtt gcggagggcg
      241 gattgatatc gtcgtgactg acaggaacgg tgtccggtgt gggatcgagc ttgaccgaaa
      301 ttctccgcga cagaa
//
LOCUS       DAJTQQ010000384          309 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000384 DAJTQQ010000000
VERSION     DAJTQQ010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.399"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..>309
                     /locus_tag="OO309_004425"
     CDS             <1..>309
                     /locus_tag="OO309_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5849277.1"
                     /translation="QLSRPSEINRLKALVAKLQRMQFGKSSEKLRAKTERWIQEAQER
                     ISALQEEMAETLGEQYDPVLPSSLRQSSARKPLPASLPRAPRVIRPEEECCPACGGEL
                     S"
BASE COUNT           78 a           90 c           86 g           55 t
ORIGIN      
        1 cagttgtcca ggccttccga aatcaaccga ctgaaggcgc tggtcgcgaa actgcagcgt
       61 atgcagttcg gtaaaagctc agaaaaactt cgcgcaaaaa ccgaacggtg gatacaggaa
      121 gcacaggagc gaatcagcgc acttcaggag gaaatggcgg aaacactggg tgagcaatat
      181 gacccggtac tgccatcttc cctgcgccag tcttcagccc gtaaaccgtt accagcctca
      241 cttccccgtg ccccccgggt catccggccg gaagaggaat gctgtccggc ctgtggtggt
      301 gaactcagt
//
LOCUS       DAJTQQ010000385          309 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000385 DAJTQQ010000000
VERSION     DAJTQQ010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.400"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..164
                     /locus_tag="OO309_004426"
     CDS             <1..164
                     /locus_tag="OO309_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic sulfatase maturase AslB"
                     /protein_id="HCR5849278.1"
                     /translation="LDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLLAHGRPASDIM
                     QAHLMVVNK"
BASE COUNT           69 a           73 c           86 g           81 t
ORIGIN      
        1 tgctcgatgc cagcggcaaa ccggggctga attatttgtg tgccgggtat cagcgttatt
       61 tccgccatct accgccatat cttaaagcaa tggctgattt gctggcgcac ggtcgcccgg
      121 ccagtgacat tatgcaggca catttgatgg tggtgaataa gtaaaaaagc ggccatttgg
      181 aagccgcctg cggttgattg ccggatgcgg cgtaaacgcc ttatccggcc tacatgatcg
      241 tgcaaattca ataaattgca gcgttctgtg gatactggtt tgggtgatcg tatccggcaa
      301 agcctttac
//
LOCUS       DAJTQQ010000386          300 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000386 DAJTQQ010000000
VERSION     DAJTQQ010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.402"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..197)
                     /locus_tag="OO309_004427"
     CDS             complement(<1..197)
                     /locus_tag="OO309_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5849279.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRL"
BASE COUNT           92 a           54 c           47 g          107 t
ORIGIN      
        1 tttagcctat tgaaggcgat attgcgttgc tcgcctgggg taccgttctt ttcccattca
       61 ctccagactt ttaaataata ttcattacaa gatggactca aactcacagg caaagaaaat
      121 agagttaacg agtgtatgtt gtttatttga tgcataatac cagaaccgat tacctgtata
      181 ttcgttgatt taatcattat aaccagaatt tcttatcaac gataagaact ctagtgaaat
      241 acaatataac ttattaagtt taatacagtt tgctttgtac tctttctcca ggtattgctt
//
LOCUS       DAJTQQ010000387          299 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000387 DAJTQQ010000000
VERSION     DAJTQQ010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.denovo.403"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..138
                     /locus_tag="OO309_004428"
     CDS             <1..138
                     /locus_tag="OO309_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR5849280.1"
                     /translation="SGDDMEALAFAWLAWRTLAGLPGNLPSVTGASQETVLGAIFPAN
                     S"
     gene            236..>299
                     /locus_tag="OO309_004429"
     CDS             236..>299
                     /locus_tag="OO309_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HCR5849281.1"
                     /translation="MTMKKLLIIILPVLLSGCSAF"
BASE COUNT           64 a           70 c           79 g           86 t
ORIGIN      
        1 agtggcgatg acatggaagc attggctttc gcctggcttg cctggcggac gctggcggga
       61 ttaccaggaa atctgccttc cgtcactggc gcaagccagg agacggtact gggggctatt
      121 ttccccgcca actcgtgata tcagaattaa ctgaattttt tcttatcgtg tctgccgtta
      181 cacttggaag attaaggagg gtatttgccc tccggaccag gaaagtcttc gggatatgac
      241 tatgaaaaaa ctgttaatta tcattttgcc ggtccttctc tccggttgta gcgccttta
//
LOCUS       DAJTQQ010000388         7206 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.guided.156, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000388 DAJTQQ010000000
VERSION     DAJTQQ010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7206
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.guided.156"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..1353
                     /gene="frdA"
                     /locus_tag="OO309_004430"
     CDS             <1..1353
                     /gene="frdA"
                     /locus_tag="OO309_004430"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR5849282.1"
                     /translation="FDEHFVLDILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGA
                     GRVYRYNTNGGIVTGDGMGMALSHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGI
                     LVNKNGYRYLQDYGMGPETPLGEPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDV
                     VYLDLRHLGEKKLHERLPFICELAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCET
                     RIKGLFAVGECSSVGLHGANRLGSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEA
                     QAAGVEQRLKDLVNQDGGENWAKIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQER
                     FKRVRITDTSSVFNTDLLYTIELSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERD
                     DVNFLKHTLAFRDADGTTRLEYSDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG
                     "
     gene            1346..2080
                     /gene="frdB"
                     /locus_tag="OO309_004431"
     CDS             1346..2080
                     /gene="frdB"
                     /locus_tag="OO309_004431"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5849283.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            2091..2486
                     /gene="frdC"
                     /locus_tag="OO309_004432"
     CDS             2091..2486
                     /gene="frdC"
                     /locus_tag="OO309_004432"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5849284.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            2497..2856
                     /gene="frdD"
                     /locus_tag="OO309_004433"
     CDS             2497..2856
                     /gene="frdD"
                     /locus_tag="OO309_004433"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5849285.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            2919..4051
                     /locus_tag="OO309_004434"
                     /pseudo
     CDS             2919..4051
                     /locus_tag="OO309_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
     gene            4140..4673
                     /gene="blc"
                     /locus_tag="OO309_004435"
     CDS             4140..4673
                     /gene="blc"
                     /locus_tag="OO309_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5849286.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(4670..4987)
                     /gene="sugE"
                     /locus_tag="OO309_004436"
     CDS             complement(4670..4987)
                     /gene="sugE"
                     /locus_tag="OO309_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5849287.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(5163..5309)
                     /gene="ecnB"
                     /locus_tag="OO309_004437"
     CDS             complement(5163..5309)
                     /gene="ecnB"
                     /locus_tag="OO309_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5849288.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(5420..5545)
                     /gene="ecnA"
                     /locus_tag="OO309_004438"
     CDS             complement(5420..5545)
                     /gene="ecnA"
                     /locus_tag="OO309_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5849289.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(5597..6163)
                     /gene="efp"
                     /locus_tag="OO309_004439"
     CDS             complement(5597..6163)
                     /gene="efp"
                     /locus_tag="OO309_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5849290.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            6205..>7206
                     /gene="epmB"
                     /locus_tag="OO309_004440"
     CDS             6205..>7206
                     /gene="epmB"
                     /locus_tag="OO309_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR5849291.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     "
BASE COUNT         1776 a         1861 c         1902 g         1667 t
ORIGIN      
        1 tttgacgaac atttcgtgct ggatattctg gttgatgatg gtcatgttcg cggcctggta
       61 gcaataaaca tgatggaagg cacgttggtg cagatccgtg ctaacgcggt cgttatggct
      121 accggcggtg cgggtcgcgt ttatcgttac aacaccaacg gcggcatcgt taccggtgac
      181 ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg acatggaatt cgttcagtat
      241 cacccaaccg gtctgccagg ttccggtatc ctgatgaccg aaggctgccg tggtgaaggc
      301 ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag attacggcat gggcccggaa
      361 actccgctgg gcgagccgaa aaacaaatat atggaactgg gtccacgcga caaagtttct
      421 caggccttct ggcacgaatg gcgtaaaggc aacaccatct ccacgccgcg tggtgatgtg
      481 gtttatctcg acctgcgtca cctcggcgag aaaaaactgc atgaacgtct gccgttcatc
      541 tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta aagaaccgat tccggtacgt
      601 ccgactgcac actacaccat gggcggtatc gaaaccgatc agaactgtga aacccgcatt
      661 aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc tgcacggtgc aaaccgtctg
      721 ggctccaact ccctggcgga actggtggtc ttcggccgtc tggccggtga acaagcgaca
      781 gagcgtgcag caactgccgg taatggcaac gaagcggcaa ttgaagcgca ggcagctggc
      841 gttgaacaac gtctgaaaga tctagttaac caggatggcg gcgaaaactg ggcgaagatc
      901 cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta tctaccgtac gccggaactg
      961 atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac gcttcaagcg cgtgcgcatc
     1021 accgacacct ccagcgtgtt caacaccgac ctgctctaca ccattgaact gagccacggt
     1081 ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac gtaaagagtc ccgcggcgca
     1141 caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg tcaactttct caaacacacc
     1201 ctcgccttcc gcgatgctga tggcacgact cgcctggagt acagcgacgt gaagattact
     1261 acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg atgcagccga taaggcggaa
     1321 gcagccaata ataaggagaa ggcgaatggc tgagatgaaa aacctgaaaa ttgaggtggt
     1381 gcgctataac ccggaagtcg ataccgcacc gcatagcgca ttctatgaag tgccttatga
     1441 cgcaactacc tcattactgg atgcgctggg ctacatcaaa gacaacctgg caccggacct
     1501 gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc tgcggcatga tggttaacaa
     1561 cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac accgacggta tgaaggttga
     1621 agcgttagct aacttcccga ttgaacgcga tctggtggtc gatatgaccc acttcatcga
     1681 aagtctggaa gcgatcaaac cgtacatcat cggcaactcc cgcaccgcgg atcagggtac
     1741 taacatccag accccggcgc agatggcaaa gtatcaccag ttctccggtt gcatcaactg
     1801 tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac ccagagttca tcggtccggc
     1861 tgccattacg ctggcgcatc gttataacga agatagccgc gaccacggta agaaggagcg
     1921 tatggcgcag ttgaacagcc agaacggtgt atggagctgt actttcgtgg gctattgctc
     1981 cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt cagcagggca aagtagaaag
     2041 ttcgaaagac tttcttatcg cgaccctgaa accacgctaa ggagtgcaac atgacgacta
     2101 aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg gaaaaaattg ccgttttatc
     2161 gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt gtggttcagc attgaactga
     2221 ttttcgcgct gtttgccctg aaaaatggcc cggaagcctg ggcgggattc gtcgactttt
     2281 tacaaaaccc ggttatcgtg atcattaacc tgatcactct gacggcagct ctgctgcaca
     2341 ccaaaacctg gtttgaactg gcaccgaaag cggccaatat cattgtaaaa gacgaaaaaa
     2401 tgggaccaga gccaattatc aaaagtctct gggcggtaac tgtggttgcc accatcgtaa
     2461 tcctgtttgt tgccctgtac tggtaaggag cctgagatga ttaatccaaa tccaaagcgt
     2521 tctgacgaac cggtattctg gggcctcttc ggggccggtg gtatgtggag cgcaattatt
     2581 gcgccggtga tgatcctgct ggtgggtatt ctgctgccac tggggctgtt tccgggtgat
     2641 gcgctgagct acgagcgcgt tctggcgttc gcgcagagct tcattggtcg cgtattcctg
     2701 ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc gtatgcacca cgcgatgcac
     2761 gatctgaaaa tccacgtgcc tgcgggcaaa tgggttttct acggtctggc tgctatcctg
     2821 acagttgtca cgctgattgg tgtcgttaca atctaacgca tcgccaatgt aaatccggcc
     2881 cgcctatggc gggccgtttt gtatggaaac cagaccttat gttcaaaacg acgctctgcg
     2941 ccttattaat taccgcctct tactccacat ttgctgcccc tcaacaaatc aacgatattg
     3001 tgcatcgcac aattaccccg cttatagagc aacaaaagat ccccggtatg gcggtggcgg
     3061 taatttatca gggtaaacct tattacttta cctggggcta tgcggacatc gccaaaaagc
     3121 agcccgtcac acagcaaacg ttgtttgagt taggttcggt cagcaaaaca tttacgggcg
     3181 tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt aagcgatccc gcaacaaaat
     3241 actggcctga acttaccgct aaacagtgga atgggatcac actattacat cttgcgacct
     3301 acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt gaaatcctca agcgacttgc
     3361 tgcgcttcta tcaaaactgg cagcctgcat gggctccagg aacacaacgt ctgtatgcca
     3421 actccagtat cggtttgttc ggcgcactgg ctgtgaagcc gtctggtttg agttttgagc
     3481 aggcgatgca aactcgtgtc ttccagccac tcaaactcaa ccatacgtgg attaatgtac
     3541 ctcccccaga agaaaagaat tacgcctggg gatatcgcga aggtaaggca gtgcatgttt
     3601 cgccaggggc gttagatgct gaagcttatg gtgtgaagtc gaccattgaa gatatggccc
     3661 gctgggtgca aagcaattta ccccccttga tatcaatgag aaaacgcttc aacaagggat
     3721 acaactggca caatctcgct actggcaaac cggcgatatg tatcagggcc tgggctggga
     3781 aatgctggac tggccggtca atcctgacag catcattaac ggcagtgaca ataaaattgc
     3841 actggcagca cgcccggtaa aagcgattaa gcccccaact cctgcagtac gcgcatcatg
     3901 ggtacataaa acgggggcga ccggcggatt tggtagctat gtcgcgttta ttccagaaaa
     3961 agagctgggt atcgtgatgc tggctaacaa aaactatccc aatccagcga gagtcgccgc
     4021 cgcctgtcag attctcaacg ctctacagta aaatttcatc gggtccgaat tttcggacct
     4081 tttctgcgct tttccttgct gtcatctaca cttagaaaaa aaccagtaag gaaacattta
     4141 tgcgcctgct ccctctcgtt gccgcagcga cagctgcatt tctggtcgtt gcctgtagtt
     4201 ttcctacgcc gccgcgtggc gtgaccgtag tgaataattt cgacgccaaa cgttatctcg
     4261 gtacctggta tgagattgcc cgttttgatc accgttttga acgtggactg gaaaaagtca
     4321 ccgcaacata cagcctgcgt gatgacggcg gcctgaatgt cattaataaa ggctataacc
     4381 ctgacagagg aatgtggcag cagagtgaag ggaaagcgta ctttaccggc gcaccaactc
     4441 gcgctgcgct gaaagtgtca ttctttggtc ctttctatgg cggttataac gttattgcac
     4501 tcgatcggga ataccgccat gcgctggttt gcggcccgga ccgcgactac ctgtggatac
     4561 tctcccgcac gccaaccatt tctgacgaag tgaaacagga gatgctggca gtcgcgaccc
     4621 gggaagggtt tgatgtcagt aagtttattt gggtacagca gcccggtagt tagtgagtgc
     4681 tgagtttcag accaataatc cccaatacga ttaacgccag actcgccaga cgcatcgggt
     4741 tagcggactc accgagcagc acaatgccgg tgatggccgc gccgactgcg ccaatacccg
     4801 tccacacggc ataagccgtc cctaccggta acgatttcat cgcccaggca agtagcgcca
     4861 tactgactat catcgccgtc acggtaataa cactcggcgt caaacggcta aagccgtggg
     4921 tatatttcag gccaacggcc cataccactt ccagcagacc agcaataact aagataatcc
     4981 aggacatatc aggctccgga aatttggggc cgtccccggt gaaaagaggc gtttgcaggt
     5041 cgtcctgcaa agctgatggt tgaacggata ttttgccagg tggcagggga atttcaacta
     5101 ttacctttat gacgatcgca gaaaaaacaa acggcacgac actgctgtcg taccgttatt
     5161 gcttattgct gcgctttcgt tgctgcgcca gaaatcgcgt taccgccatc agaaatgtct
     5221 tcaccaacgc cacgcgtggt gttgcaggca gttaatactg ttgaaagcac cagaacagaa
     5281 aagatcgctg caattgtctt cttcaccata acgtcttcct tttaggtaat gttatttatg
     5341 tttgcctata gcaaattaat aatagacaac atcgccaaaa atgatggaaa tctgatgatt
     5401 tttcggaaga gaagaaaaat tagctggcag cgcgagagat ggagttaccg agatgtttga
     5461 tgtcttcgcc gaaaccgcaa gcggtgttac agcccgtgag cagcgtgctg gcaagcaaaa
     5521 caagaacgat aaggcgtttc atcatctctg acctgaataa gtgatggtgc agcctgcagg
     5581 ccgcaccaca accgcattac ttcacgcgag agacgtattc accagagcgg gtatccactt
     5641 tgatgacttc gccgatttgt acgaacagcg gaactttaac cacagcgcca gtagacaggg
     5701 tagccggttt gccgccagta cctgcggtat cacctttcag gcccggatcg gtatcaacga
     5761 tttccagttc aacgaagttc ggcggagtaa cggagatcgg ctgaccattc cacagagtta
     5821 cgatacactc tgcctgatcc agcagccatt tagcattgtc gccaattgct tttgcatcag
     5881 cagacagctg ctcgaaagtt tcgttgttca tgaagtgcca gaactcaccg tcgttgtaca
     5941 ggtaagtcag gttcatatcg acaacatcag cgccttcagc ggaatcagta gatttgaagg
     6001 ttttttctac gcgagtaccg gtcagcagac gacgcagttt aacgcgagca aatgcctggc
     6061 ctttacccgg ttttacgaat tcactcgctt caaccgcgta aggttcgccg tctaacatga
     6121 ttttaagacc agcacgaaaa tcgttgctat agtacgttgc cataaggccc tctgaaattt
     6181 gttaattggt agctaagcca caaaatggcg catattgtaa ccctaaatac cccatccaga
     6241 gaagattggt taacgcaact tgccgatgtt gtgaccgatc ctgatgaact tctgcgtctt
     6301 ttgaatatag acgcggacga aaaactgttg gccggacgca gcgccaaaaa gctgtttgcc
     6361 ctgcgtgtgc cccgctcatt tatcgatcgc atggagaaag gcaatccgga cgatcctctt
     6421 ttgcgtcagg tacttacctc gcaagatgag tttgtcgtcg cgtccggatt ctccaccgac
     6481 ccactggaag aacagcacag cgtagtgcct ggtttgttgc ataaatacca caaccgggcg
     6541 cttttgctgg tcaaaggcgg ctgcgcggta aattgccgct attgcttccg tcgccacttc
     6601 ccctatgccg aaaatcaggg caacaagcgt aactggcaga ctgcacttga gtatgttgct
     6661 gcgcatccgg aactggacga gatgattttc tccggcggcg atccgctgat ggcgaaagag
     6721 cacgagctgg actggttgct cacacaactg gaagccatcc cgcatatcaa acgtctgcgg
     6781 attcacagcc gtctgccgat tgtgatcccg gcacgtatca ccgaggcgct ggttgaacgc
     6841 tttgcccgtt ctacgctgca aatcttgctg gtgaatcaca tcaatcatgc caatgaggta
     6901 gatgaaacgt tccgtcaggc gatggctaaa ttgcgccgtg tcggtgtcac cctgcttaac
     6961 cagagcgttc tgttacgtgg tgtgaacgat aacgcacaaa cgttggcaaa cctgagtaat
     7021 gcgttgttcg atgccggcgt gatgccgtat tacctgcatg tgctagataa agtacagggc
     7081 gcggcgcatt ttatggtgag tgatgacgaa gcacggcaga ttatgcgtga gttgctgaca
     7141 ttggtgtcgg gatatctggt gccgaaactg gcgcgagaaa ttggcggcga acccagcaaa
     7201 acgccg
//
LOCUS       DAJTQQ010000389         5962 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.guided.167, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000389 DAJTQQ010000000
VERSION     DAJTQQ010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.guided.167"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            720..1535
                     /gene="sul2"
                     /locus_tag="OO309_004441"
     CDS             720..1535
                     /gene="sul2"
                     /locus_tag="OO309_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5849292.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1596..2399
                     /locus_tag="OO309_004442"
                     /note="aph(3'')-Ib"
     CDS             1596..2399
                     /locus_tag="OO309_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR5849293.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2399..3232
                     /locus_tag="OO309_004443"
                     /pseudo
     CDS             2399..3232
                     /locus_tag="OO309_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(3342..3746)
                     /locus_tag="OO309_004444"
                     /pseudo
     CDS             complement(3342..3746)
                     /locus_tag="OO309_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(3790..4179)
                     /locus_tag="OO309_004445"
     CDS             complement(3790..4179)
                     /locus_tag="OO309_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849294.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4397..4837
                     /locus_tag="OO309_004446"
     CDS             4397..4837
                     /locus_tag="OO309_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5849295.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(5582..5731)
                     /locus_tag="OO309_004447"
     CDS             complement(5582..5731)
                     /locus_tag="OO309_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5849296.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
BASE COUNT         1452 a         1570 c         1551 g         1389 t
ORIGIN      
        1 cctttcggtt taccggtgtc attccgctgt tatggccgcg gtttctcatt ccacgcctga
       61 cactcagttc cgtgaaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
      121 agtccgaccg ctgcgccttg tccggtaact atcaacttga gtccaacccg gaaagacacg
      181 acaaaacgcc actggcagca gccattagta acaggagtgc acgagaagat agattaagag
      241 gtcttgaagt agaggcctga ctgcggctac actgaaagaa cagttttggt aatggcggtc
      301 tcgtgaaccc tgttaccggg ttaagcagtt ccccaactga cttaaccttc gaacaaaccg
      361 cctccccagg cggttttttc gtttacagag caagagatta cgattcattg aaaaggatct
      421 caagaagatc ctttgtagat tactatcaat taatcttccg aacatgacac cagtcactgt
      481 tgattacggt taaaccgctc cgtaacaggt ttccctcgta ttggtgactt gcagcaccaa
      541 agaagcaaaa aagataaata tatctactta ttgagagatg atatatttat ttccaaaatg
      601 ctacagatac atcatcatga aacggatcac cgcaaacagg ttactcgcct ggggattccc
      661 tttcgacccg agcatccgta tgagactcat gctcgattat tattattata gaagccccca
      721 tgaataaatc gctcatcatt ttcggcatcg tcaacataac ctcggacagt ttctccgatg
      781 gaggccggta tctggcgcca gacgcagcca ttgcgcaggc gcgtaagctg atggccgagg
      841 gggcagatgt gatcgacctc ggtccggcat ccagcaatcc cgacgccgcg cctgtttcgt
      901 ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc gctcaaggca gatggcattc
      961 ccgtctcgct cgacagttat caacccgcga cgcaagccta tgccttgtcg cgtggtgtgg
     1021 cctatctcaa tgatattcgc ggttttccag acgctgcgtt ctatccgcaa ttggcgaaat
     1081 catctgccaa actcgtcgtt atgcattcgg tgcaagacgg gcaggcagat cggcgcgagg
     1141 cacccgctgg cgacatcatg gatcacattg cggcgttctt tgacgcgcgc atcgcggcgc
     1201 tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga tcccggcatg gggttttttc
     1261 tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg gttcgatgaa ttgcggctgc
     1321 gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc ctttctgcgc gcgctcacag
     1381 gccgtggtcc gggggatgtc ggggccgcga cactcgctgc agagcttgcc gccgccgcag
     1441 gtggagctga cttcatccgc acacacgagc cgcgcccctt gcgcgacggg ctggcggtat
     1501 tggcggcgct gaaagaaacc gcaagaattc gttaactgca cattcgggat atttctctat
     1561 attcgcgctt catcagaaaa ctgaaggaac ctccattgaa tcgaactaat attttttttg
     1621 gtgaatcgca ttctgactgg ttgcctgtca gaggcggaga atctggtgat tttgtttttc
     1681 gacgtggtga cgggcatgcc ttcgcgaaaa tcgcacctgc ttcccgccgc ggtgagctcg
     1741 ctggagagcg tgaccgcctc atttggctca aaggtcgagg tgtggcttgc cccgaggtca
     1801 tcaactggca ggaggaacag gagggtgcat gcttggtgat aacggcaatt ccgggagtac
     1861 cggcggctga tctgtctgga gcggatttgc tcaaagcgtg gccgtcaatg gggcagcaac
     1921 ttggcgctgt tcacagccta tcggttgatc aatgtccgtt tgagcgcagg ctgtcgcgaa
     1981 tgttcggacg cgccgttgat gtggtgtccc gcaatgccgt caatcccgac ttcttaccgg
     2041 acgaggacaa gagtacgccg cagctcgatc ttttggctcg tgtcgaacga gagctaccgg
     2101 tgcggctcga ccaagagcgc accgatatgg ttgtttgcca tggtgatccc tgcatgccga
     2161 acttcatggt ggaccctaaa actcttcaat gcacgggtct gatcgacctt gggcggctcg
     2221 gaacagcaga tcgctatgcc gatttggcac tcatgattgc taacgccgaa gagaactggg
     2281 cagcgccaga tgaagcagag cgcgccttcg ctgtcctatt caatgtattg gggatcgaag
     2341 cccccgaccg cgaacgcctt gccttctatc tgcgattgga ccctctgact tggggttgat
     2401 gttcatgccg cctgtttttc ctgctcattg gcacgtttcg caacctgttc tcattgcgga
     2461 caccttttcc agcctcgttt ggaaagtttc attgccagac gggactcctg caatcgtcaa
     2521 gggattgaaa cctatagaag acattgctga tgaactgcgc ggggccgact atctggtatg
     2581 gcgcaatggg aggggagcag tccggttgct cggtcgtgag aacaatctga tgttgctcga
     2641 atatgccggg gagcgaatgc tctctcacat cgttgccgag cacggcgact accaggcgac
     2701 cgaaattgca gcggaactaa tggcgaagct gtatgccgca tctgaggaac ccctgccttc
     2761 tgcccttctc ccgatccggg atcgctttgc agctttgttt cagcgggcgc gcgatgatca
     2821 aaacgcaggt tgtcaaactg actacgtcca cgcggcgatt atagccgatc aaatgatgag
     2881 caatgcctcg gaactgcgtg ggctacatgg cgatctgcat catgaaaaca tcatgttctc
     2941 cagtcgcggc tggctggtga tagatcccgt cggtctggtc ggtgaagtgg gctttggcgc
     3001 cgccaatatg ttctacgatc cggctgacag agacgacctt tgtctcgatc ctagacgcat
     3061 tgcacagatg gcggacgcat tctctcgtgc gctggacgtc gatccgcgtc gcctgctcga
     3121 ccaggcgtac gcttatgggt gcctttccgc agcttggaac gcggatggag aagaggagca
     3181 acgcgatcta gctatcgcgg ccgcgatcaa gcaggtgcga cagacgtcat actatatatc
     3241 aagcgacttc tcctatcccc tgggaacaca tcaatctcac cggagaatat cgctggccaa
     3301 agccttagcg taggattccg ccccttcccg caaacgaccc caaacaggaa acgcagctga
     3361 aacgggaagc tcaacaccca ctgacgcatg ggttgttcag gcagtacttc atcaaccagc
     3421 aaggcggcac tttcggccat ccgccgcgcc ccacagctcg ggcagaaacc gcgacgctta
     3481 cagctgaaag cgaccaggtg ctcggcgtgg caagactcgc agcgaacccg tagaaagcca
     3541 tgctccagcc gcccgcattg gagaaattct tcaaattccc gttgcacata gcccggcaat
     3601 tcctttccct gctctgccat aagcgcagcg aatgccgggt aatactcgtc aacgatctga
     3661 tagagaaggg tttgctcggg tcggtggctc tggtaacgac cagtatcccg atcccggctg
     3721 gccgtcctgg ccgccacatg aggcatgttc cgcgtccttg caatactgtg tttacataca
     3781 gtctatcgct tagcggaaag ttcttttacc ctcagccgaa atgcctgccg ttgctagaca
     3841 ttgccagcca gtgcccgtca ctcccactct tacattgttc gcagtatcta ggtacatgtt
     3901 attgcgtgca ccttcatgat gtataatgtt attaatgaca gcagaaagat cgacagaatt
     3961 aatattcttc gtacctggga cagttatcgg gtgtcggcca gtgatgagac caaaggatgc
     4021 agtataagat gaaccactac tattatcatg gccaccatta ccaccaccgt taaaactacc
     4081 tcccgaactt ccagagccgc tatgaccatc attgtagggg tcatttggtc ccatgtgacc
     4141 aaaaccaccg acagtaccgc tatttaactc tgtactcata aaattatcct ctttgacttt
     4201 taaaaacagc aaaaactgta tttataacaa tgtgtttata tacagtattc gaaatactac
     4261 gcccgtcaaa accacaccgt caacaggttt tttgtgtatt tttcacgcct cacgggcagc
     4321 ctcagacctt ttgtgttaaa acagtaatat gttctgctgt tacaatacgg tacacacagg
     4381 agacactgtc acttttatgg catggcacac agttcaagag acactgaaat taacaggaaa
     4441 gtcgcgctcg caattatatc gtgacatggc taaaggcctt gtcagctatc gcacaggaaa
     4501 aaatgatcgc cgggagttcg agaccagtga actgattcgg gcctatggcg agctgcgcac
     4561 ttctgagaca ccaaaaaggc acaaggaggg acactccaaa tctgtcgtta gccaggcgga
     4621 aaatgctcag ctagacgtca ttcagcagaa actggacgct ctgcagcaga ctgttaccct
     4681 aatgcttgaa gacaagatgg cgcgggaagc agaaaaccgc ctgcgaaatg aggagaggga
     4741 acaactacag gccgaaatta gccgtctggc tgacgcgctg gcacaggaaa aaaagcgggg
     4801 attctggtcg cggctgtttc gctccggttc agagtaacag tgcaggtgac tctgatttac
     4861 cataacatct gttatcagca ccgcctgaga ggccctcagg gcgtttctga cgggaaaccg
     4921 gtaaaacagg caacaaacca cccgaaaacc cgccagaaac gcgcaggctc tgtttttacg
     4981 gcatgcatga gtatgcgcac aagtgcatag cgcggaaatt cacaccattc agccggggta
     5041 agtcctgttt ccggcgttct tcccatccga aaacggtgaa cggggacgca atttattgcg
     5101 tggccgtgaa gcgttgccgg cgcagccgga aaacccgctg tcagcacgtt caggaagggg
     5161 gtgatgtaca aatggcacag aggatgaaag tgacgtatga gctgaaggaa tgatgtaaaa
     5221 gtgatgtata agtgatgtaa aagtgatgta tgaactgaag gagcgatgta attctgtagt
     5281 caggaagaat aacccgttca tgcaggcatg aacgatacag tgaggaacgc tttttttgtc
     5341 acgatctccc cttaacctgc gtaaatcacc cgccatggtt gattattttt caacctcaaa
     5401 atggtcatac acctaacgca gccgcgattt tcatcgcaac tgcatgaatt aaaagaaaaa
     5461 aaaggaatat ttcgttttca cgagtaatac ccgggtatta aaaatccact gtaattacat
     5521 gaaaataaag aaataaaagg taataccgga gtattaccac gtacgcgggc aagcgaaaaa
     5581 gctataacgc ttcccggccg acaggcgacc caaaatccac aggggcatgc aggttctctt
     5641 cagtaacctg cgccattaaa tcaaaaaggt catattcgcc gggaagctgc gtatcagtcg
     5701 aatccagttg ttcatcatat tcagatggca tggggtgata tccagtagct gagaggttgt
     5761 tagcgggttt cggggcgcag ccctgaacca gtcatgtagc gctagcggag tgtatactgg
     5821 cttactatgt tgcaacgatt gcgaaaatca gtgaagtgct tcatgtggca ggagaaaaaa
     5881 ggcagcaccg gcgcgtcagc agaatatgcg atacaggaga tattccgctt cctcgctcac
     5941 tgactcgcta cgctcggtcg tt
//
LOCUS       DAJTQQ010000390         5076 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.guided.184, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000390 DAJTQQ010000000
VERSION     DAJTQQ010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.guided.184"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..1770)
                     /locus_tag="OO309_004448"
     CDS             complement(<1..1770)
                     /locus_tag="OO309_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR5849297.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTG"
     gene            complement(1773..2333)
                     /locus_tag="OO309_004449"
     CDS             complement(1773..2333)
                     /locus_tag="OO309_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5849298.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(2459..2809)
                     /locus_tag="OO309_004450"
     CDS             complement(2459..2809)
                     /locus_tag="OO309_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5849299.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(3012..4025)
                     /gene="intI1"
                     /locus_tag="OO309_004451"
     CDS             complement(3012..4025)
                     /gene="intI1"
                     /locus_tag="OO309_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5849300.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            4235..5065
                     /gene="blaOXA"
                     /locus_tag="OO309_004452"
     CDS             4235..5065
                     /gene="blaOXA"
                     /locus_tag="OO309_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-1 family class D beta-lactamase"
                     /protein_id="HCR5849301.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAVTILNTLNL
                     "
BASE COUNT         1140 a         1412 c         1450 g         1074 t
ORIGIN      
        1 gcccgtccac tcatcgactt cgagcagcag ttcggtgatc ttgacgtgcg gcaggaccat
       61 ggctgtctgg tctatcagcg cctgcgcggt gtcgggcacc gccgcatcca gcggcgtgat
      121 cttcaagccc gactcggtga tgatggcatc cggcaggtcg ttggctgccg ccatgcggtt
      181 gacggtggca agttgtgctt ccagcagcgt cagccgctca tgcagatatt gttcgcagtc
      241 ggtggccacg gccagcggca attcgctgga ctgcttgagg ctggtgaact tctcgggcgg
      301 taccaggtag tcctcgaagt ccttgaactg gcgtgaaccc tgcacccaga tgtcgcccga
      361 gcgcagggag ttcttcaact cggacagcgc gcacagttcg tagtagcgcc ggtcgatgcc
      421 ggcgtcggtc atcaccagtt tctgccagcg cggcttgatg aagccggtcg gtgcatcggc
      481 tggcagcttg cgggcgttgt cggtgttcat gccgcgcagc acctcaatgg catcaagcac
      541 gtttttggcg gcgggcgcgg cccgcagctt gagcacggca aggaattccg gtgcatagcg
      601 gcgcagggtg gcgtagctct cgccgatgcg atgcaggaaa tcgaagtcat cgggttgcgc
      661 gagcttctgc gcctcggtga cgctctcggc aaaggaatcc caggacatga cggcctcgat
      721 ggcggcaaac gcatcgcggc ctgattgctt ggcgtcgatc agcgcctgac cgatgcgccc
      781 gtacagacgt accttggcgt tgatggcctt gcctgacgcc tggaactgct gctgatgctt
      841 attcttggca gcgttaaaca gcttacccag gatgcggtcg tgcaggtcga tgatttcgtc
      901 ggtgacggtg gccatgccct cggtggccag cgccacgaga gtggcgtagc gccgttgcgg
      961 ctcgaatttg gccaggtcgg cgggtgtcat ctggccgccc tcgcgggcaa tcttgagcag
     1021 gcggttctgg tgaaccagcc gctcgatgcc ggtaggcaga tcgagtgcct gccatgcctt
     1081 gaggcgttcg atgtgttcca gcatatgccg cgaatttggc ttggccggag actggcgcaa
     1141 ccaagccaac caggtcgtct tgccgttgtc ccggcgcttg agcagatcgt cgaggcggcg
     1201 gcgatgcgcg tccgccagtg gttcggccaa ggcgtcgtag atgcgccggt tagcacgggt
     1261 gatcgcctcg gcactcgccc gctcgacggc gttgagggcg ggcagaatga ccgactgccg
     1321 ccgcaggtgc ccgatcaagg cgctggccag cacgatgcct ttgtcggttt gcatcgccag
     1381 ctcggtcagc atctggacgg cctgccggta atggctcatg gtgaagggcc ggaaaccgaa
     1441 cacggtttgc agctcgctca ggtgctcgcg ccgggtctgc tcccgctggc cgtactcgtt
     1501 ccagctttcg acgccgacct tgagctggtc ggcgaccagc ttcaacaagg gcgggaacgg
     1561 tagttcatcg acgcccagga tgacgccggg aaagcgcagg taacagagct gcaccgcgaa
     1621 gcccagccga ttggctggcc cgcgccgctg tcggatgatc gagaggtcgg tatcgttgaa
     1681 tgtgtaatgt cggatcaggt cgtccttgga gtccggcaac gccagcaggc tttcccgctc
     1741 ggcggcggac aggatggaac gacgtggcat atttactgat ccgttctcaa gtattgatac
     1801 agggtttcgc gactgattcc gaattcacga gcaagcttgg tcttttgctc gccagcctcg
     1861 acacgttggc gcagttcggc aatacgctca gacgacaggg atttcttcct gccacggtaa
     1921 gccccgcgtt gcttggcgag cgcaataccc tcgcgctgac gctcgcggat cagggcgcgc
     1981 tcgaactcgg cgaacgcgcc catcaccgag agcatcaggt tcgccatcgg agagtcttcg
     2041 ccagtaaaac tgaggtgttc cttgacgaat tcgatatgca cgccgcgttg tgtcagcgtt
     2101 tgcacgatcc ggcgcaaatc atcgagattg cgcgccaggc gatccatgct atgcaccacc
     2161 acggtgtcgc cggtgcgggc gaagcttatc agcgcttcca gttgcggacg cttgacatcc
     2221 ttgccggatg ccttgtcgct aaaagcgcga tcaaccttga cgccttccag ttgccgttcc
     2281 gggttctggt cgaaggtgct gaccctgata tacccaatgc gctgtccagt catggaattc
     2341 cctgcaaaat gtcagggaag actctatgac cttcaacgag atatgtcaat aaattcaaaa
     2401 ttcaatccta tcctgacgca atttacacat ggcatctgac atcaggttag ggtatgcctc
     2461 aacctgacgg cggcgaatca caagcgtccg gtttgacgct ggtttcggtc gcagtgctgg
     2521 cccgcgcctg gaagcgctga tagcactcca gcccgcagaa atgctccacg tactcggccc
     2581 cttccggcgt gaaggcggca tcgagcggga tttccttgca gcacacgcag caactggtgc
     2641 tcggttcatt ggcgttcatg gtggtgttcc tccattggtt gacgaagccg acgaaggccg
     2701 ccgccggcat cggcctggcg aacaggaaac cctgcaccgt gtcgcaaccc gcctgccgca
     2761 accacgcaag gcagtcgggt gtttccacac cctcggctac cacctccatt gtcgttttca
     2821 gaagacggct gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc
     2881 catcaggcaa cgacgggctg ctgccggcca tcagcggacg cagggaggac tttccgcaac
     2941 cggccgttcg atgcggcacc gatggccttc gcgcaggggt agtgaatccg ccaggattga
     3001 cttgcgctgc cctacctctc actagtgagg ggcggcagcg catcaagcgg tgagcgcact
     3061 ccggcaccgc caactttcag cacatgcgtg taaatcatcg tcgtagagac gtcggaatgg
     3121 ccgagcagat cctgcacggt tcgaatgtcg taaccgctgc ggagcaaggc cgtcgcgaac
     3181 gagtggcgga gggtgtgcgg tgtggcgggc ttcgtgatgc ctgcttgttc tacggcacgt
     3241 ttgaaggcgc gctgaaaggt ctggtcatac atgtgatggc gacgcacgac accgctccgt
     3301 ggatcggtcg aatgcgtgtg ctgcgcaaaa acccagaacc acggccagga atgcccggcg
     3361 cgcggatact tccgctcaag ggcgtcggga agcgcaacgc cgctgcggcc ctcggcctgg
     3421 tccttcagcc accatgcccg tgcacgcgac agctgctcgc gcaggctggg tgccaagctc
     3481 tcgggtaaca tcaaggcccg atccttggag cccttgccct cccgcacgat gatcgtgccg
     3541 tgatcgaaat ccagatcctt gacccgcagt tgcaaaccct cactgatccg catgcccgtt
     3601 ccatacagaa gctgggcgaa caaacgatgc tcgccttcca gaaaaccgag gatgcgaacc
     3661 acttcatccg gggtcagcac caccggcaag cgccgcgacg gccgaggtct tccgatctcc
     3721 tgaagccagg gcagatccgt gcacagcacc ttgccgtaga agaacagcaa ggccgccaat
     3781 gcctgacgat gcgtggagac cgaaaccttg cgctcgttcg ccagccagga cagaaatgcc
     3841 tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca
     3901 cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc
     3961 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
     4021 ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag
     4081 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
     4141 cagggcagtc gccctaaaac aaagttgggc gaacccggag cctcattaat tgttagccgt
     4201 taaaattaag ccctttacca aaccaatact tattatgaaa aacacaatac atatcaactt
     4261 cgctattttt ttaataattg caaatattat ctacagcagc gccagtgcat caacagatat
     4321 ctctactgtt gcatctccat tatttgaagg aactgaaggt tgttttttac tttacgatgc
     4381 atccacaaac gctgaaattg ctcaattcaa taaagcaaag tgtgcaacgc aaatggcacc
     4441 agattcaact ttcaagatcg cattatcact tatggcattt gatgcggaaa taatagatca
     4501 gaaaaccata ttcaaatggg ataaaacccc caaaggaatg gagatctgga acagcaatca
     4561 tacaccaaag acgtggatgc aattttctgt tgtttgggtt tcgcaagaaa taacccaaaa
     4621 aattggatta aataaaatca agaattatct caaagatttt gattatggaa atcaagactt
     4681 ctctggagat aaagaaagaa acaacggatt aacagaagca tggctcgaaa gtagcttaaa
     4741 aatttcacca gaagaacaaa ttcaattcct gcgtaaaatt attaatcaca atctcccagt
     4801 taaaaactca gccatagaaa acaccataga gaacatgtat ctacaagatc tggataatag
     4861 tacaaaactg tatgggaaaa ctggtgcagg attcacagca aatagaacct tacaaaacgg
     4921 atggtttgaa gggtttatta taagcaaatc aggacataaa tatgtttttg tgtccgcact
     4981 tacaggaaac ttggggtcga atttaacatc aagcataaaa gccaagaaaa atgcggtcac
     5041 cattctaaac acactaaatt tataaaaaat ctaatg
//
LOCUS       DAJTQQ010000391         4929 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.guided.186, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000391 DAJTQQ010000000
VERSION     DAJTQQ010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.guided.186"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..1431)
                     /gene="pepD"
                     /locus_tag="OO309_004453"
     CDS             complement(<1..1431)
                     /gene="pepD"
                     /locus_tag="OO309_004453"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5849302.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTE"
     gene            1693..2151
                     /gene="gpt"
                     /locus_tag="OO309_004454"
     CDS             1693..2151
                     /gene="gpt"
                     /locus_tag="OO309_004454"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5849303.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2243..3487
                     /gene="frsA"
                     /locus_tag="OO309_004455"
     CDS             2243..3487
                     /gene="frsA"
                     /locus_tag="OO309_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5849304.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            3545..3946
                     /gene="crl"
                     /locus_tag="OO309_004456"
     CDS             3545..3946
                     /gene="crl"
                     /locus_tag="OO309_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5849305.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(3985..>4929)
                     /gene="phoE"
                     /locus_tag="OO309_004457"
     CDS             complement(3985..>4929)
                     /gene="phoE"
                     /locus_tag="OO309_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR5849306.1"
                     /translation="VKAMHYMSDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEF
                     AGNKAESDTAQQKTRLAFAGLKYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQ
                     TDNFMTKRASGLATYRNTDFFGVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDF
                     GGSDFAISGAYTNSDRTNEQNLQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMT
                     PITGGFANKTQNFEAVAQYQFDFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYY
                     FNKNMSAFVDYKINQLDSDNKLNINNDDTVAVGMTYQF"
BASE COUNT         1186 a         1234 c         1238 g         1271 t
ORIGIN      
        1 ttcagtcagc agtgtccagt aatgacctac gctttcgatg tgaacttgct catccggaga
       61 gtgtggaccg gtgatagttg gcccgataga aaccatgtcc atttccggat acggtttttt
      121 gaacagacca cattccaggc ccgcgtggat aatctggatg ttcggcgtct tgttgaacag
      181 gcgctgatag gtttcacgta ccagatgcat caccggagaa ttagcgtccg gctgccagcc
      241 aggatatgcg cctttcgctt cggttttcgc gccagccagt ttacccagcg aatccagcat
      301 gctcaccacg tagtctttac cgctgtcgat tagtgaacgg atcaggcagt gaatttctac
      361 gttattgtca gtcatggtca ccacaccgac gttcagggag gtttcgacca caccttttgc
      421 cacgtcggaa ttgcggatca caccgttcgg ggtggcgttc agcagacgaa taaaggtatc
      481 gcgagatttc gcaatcaggg cagctttatc gttcgctaca gagtccagca acaaggccag
      541 atttttctct ttctctgcca gctcgttttt caggatctcc tgataggtat tcaccagaga
      601 tttcagtgcg tcgactttat cagctgcgac agcaatggtc gcaaaggctt cacgcgggat
      661 ggcgttacgc agtgtgccgc cgttgaaatc gataaggcgc aggtccagtt cttctgcatg
      721 acccgccagg aagcgcacca gcagtttgtt ggcattaccc aggccaacgt ggatttcacc
      781 gccggagtga ccgcctttca gaccttttaa ggttaacttg aaggtttcaa aaccagctgg
      841 aaccgcttca cgatctaaat gcaggttgga ggtgaagtcg ataccccccg cacaacccat
      901 gtagatttca ccttcttctt cggagtcggt gttaatcaga atatcagcct gcaaccagtt
      961 gctctgtaag ccgaacgcac cgtccatacc ggcttcttcg gtcatggtca gcagcacttc
     1021 cagcgggccg tgaaccacgt tttcgtcagc cagaaccgcc agtgcagagg ccataccaat
     1081 gccgttatcc gcacccagcg tggtgccgcg cgctttaacc cattcgccat caatataagg
     1141 ctggatagga tctttcgtga agtcatgcac ggtgtcgtta tttttctgcg gcaccatatc
     1201 gaggtgggcc tgtaagacga ccggtttacg attttccata cctgcggtag caggttgacg
     1261 aatcaggata ttacctacct gatcgcgttc gacatggaaa cctttctctt ttgcccaacc
     1321 aacaatgtat tcagcgagtt gctcttcatg ataggacggg tgaggaatag aacagatttt
     1381 ggcaaaaata tcccacagcg gctgtggaga taattgagac agttcagaca cgttaagtct
     1441 ccttgtcgat cacccgcaaa acagtattgc aggtcacagg gttagcagaa aatgttgtca
     1501 acacaagaca ggcttgcgag atatgtattg agaataccac tttatcccgc gtcagggaga
     1561 ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc
     1621 tataatctcg cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct
     1681 gggacacttc acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca
     1741 cgtaaactcg caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc
     1801 cgtggcggtc tggtaccggg ggcgttactg gcgcgtgaac tgggtattcg tcatgtcgat
     1861 accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc
     1921 gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact
     1981 gctgttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg
     2041 gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggatcgaa
     2101 cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa
     2161 cgcctggcat tgccgggcgt tgttcttttt aacttcaggc aggttacaat agtttccagt
     2221 aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc
     2281 ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg gcgcacaacc
     2341 gcctgtgcag tcggctcttg atggtaaaac catccctcac tggtatcgca tgattaaccg
     2401 tctgatgtgg atctggcgcg gtattgaccc acgtgaaatc ctcgacgtcc aggcacgtat
     2461 tgtgatgagc gatgctgaac gtaccgacga tgatttatac gataccgtga ttggctaccg
     2521 tggtggcaac tggatttatg agtgggccac ccaggcgatg gtgtggcaac aaaaagcctg
     2581 tacggaagaa gatccgcaac tcagtggtcg acactggctg catgcggcta cgttgtacaa
     2641 cattgccgcc tatcctcatc tgaaaggaga tgacctggcc gagcaagcgc aggctttatc
     2701 aaaccgcgcc tatgaagagg ccgcccagcg tctgccgggc acgatgcggc agatggagtt
     2761 taccgtaccc ggcggtgcgc ccatcaccgg ctttttgcat atgccgaaag gcgatggccc
     2821 gttcccgaca gtattaatgt gtggtggtct ggatgcgatg cagacggact attacagcct
     2881 gtatgaacgt tattttgcgc cgcgcggcat tgcgatgctg actattgata tgccgtcggt
     2941 gggcttttct tcaaaatgga agctcaccca ggactccagc ctgttgcatc agcacgtctt
     3001 aaaggcgctg cctaacgtac cgtgggtgga tcacactcgc gtcgcggcct ttggtttccg
     3061 tttcggcgct aacgttgccg tgcgtctggc ataccttgaa tcgccgcgtc tgaaagcggt
     3121 tgcctgtctt ggtccggtag ttcataccct gttgagtgat tttaagtgcc agcaacaggt
     3181 gccggaaatg tatcttgacg ttctggcgag tcgtttgggg atgcatgatg cttccgacga
     3241 agcgttacgc gtggagctga atcgctattc attaaaagtg caaggattgc tggggcgtcg
     3301 ctgcccaacg ccaatgttat caggctactg gaagaacgat ccgttcagcc cggaagagga
     3361 ctcacgctta atcacctcat catctgctga cggtaaatta ttagagatcc catttaaccc
     3421 ggtgtatcgg aattttgaca aaggtcttca ggaaatcacc ggctggatcg aaaaacgctt
     3481 gtgttaaaaa tttgctaaat tttgccaatt tggtaaaaca gttgcatcac aacaggagat
     3541 agcaatgacg ttaccgagtg gacacccgaa gagcagattg atcaaaaaat ttaccgcact
     3601 aggcccgtat attcgtgaag gtaagtgcga agataatcga ttctttttcg attgtctggc
     3661 tgtatgcgtc aacgtgaaac cggcaccgga agtgcgtgaa ttctggggct ggtggatgga
     3721 gctggaagcg caggaatccc gttttaccta cagctaccag tttggtctgt tcgataaagc
     3781 aggcgactgg aagagtgttc cggtaaaaga tactgaagtg gttgaacgac tggagcacac
     3841 cctgcgtgag tttcacgaga agctgcgtga actgctgacg acgctgaatc tgaagctgga
     3901 accggcgaat gattttcgtg atgaaccggt gaagttaacg gcgtgagtga aatgtgccgg
     3961 atgcatcaca tccggcaata ttcattaaaa ctgatacgtc atcccaaccg cgacagtatc
     4021 atcattatta atattcagct tattatcgct atccagttgg ttgattttat aatcaacaaa
     4081 cgctgacata tttttgttga aataatacgt agcaccgacg tcgatataat tgaccagatc
     4141 ttcatcaccg atgccctcaa tatctttccc tttcgataag acataaccca gcgatggacg
     4201 cagaccaaag tcaaactggt attgagcgac cgcttcaaag ttctgtgtct tattggcaaa
     4261 gccgccagtt attggcgtca ttttgcgtgt ttcagaatag aaagttgcca gataaatatt
     4321 attggcatcg tatttcagac ctgttgccca tgcttctgca cgcttgcctg tgccacggct
     4381 ttgcaggttc tgctcgttgg tgcgatctga gttggtatag gccccactaa tggcgaaatc
     4441 gctgccgcca aagtcatatg tcaatgacgt gccgaagcca tcgccgtttt gctttttaac
     4501 gtcgcggttt tcgtttttcc cttgatattg cagggttaag ttcaggccat cgataacgcc
     4561 gaagaagtcg gtgttccgat acgtcgccag accgctggcg cgtttggtca taaagttgtc
     4621 ggtctgcgcc gaggaatcgc caccaaattc cgggaacata tcggtccagg cttccacgtc
     4681 atacaacgcg cccaggttac gaccataatc gaaagaaccc aaatctttat atttcaaccc
     4741 ggcaaaagcg agacgcgttt tttgctgtgc agtatcactc tccgctttat ttccggcaaa
     4801 ctccgcttcc caccgaccat aaccagtcag ttgatcgtta atttgagttt cacctttaaa
     4861 accaaaacgg atataactct ggtcgccatc tttactggcg ttatcactca tataatgcat
     4921 ggctttaac
//
LOCUS       DAJTQQ010000392         2432 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.guided.232, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000392 DAJTQQ010000000
VERSION     DAJTQQ010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.guided.232"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(<1..74)
                     /locus_tag="OO309_004458"
     CDS             complement(<1..74)
                     /locus_tag="OO309_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCR5849307.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEG"
     gene            156..1361
                     /gene="tet(B)"
                     /locus_tag="OO309_004459"
     CDS             156..1361
                     /gene="tet(B)"
                     /locus_tag="OO309_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5849308.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1474..2067)
                     /gene="tetC"
                     /locus_tag="OO309_004460"
     CDS             complement(1474..2067)
                     /gene="tetC"
                     /locus_tag="OO309_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5849309.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            2155..>2432
                     /locus_tag="OO309_004461"
     CDS             2155..>2432
                     /locus_tag="OO309_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5849310.1"
                     /translation="MYIEQHSRYQNKANNIQLRYDDKQFHTTVIKDVLLWIEHNLDQS
                     LLLDDVANKAGYTKWYFQRLFKKVTGVTLASYIRARRLTKAAVELRLT"
BASE COUNT          651 a          430 c          506 g          845 t
ORIGIN      
        1 aaaccttcga ttccgacctc attaagcagc tctaatgcgc tgttaatcac tttactttta
       61 tctaatctag acatcattaa ttcctaattt ttgttgacac tctatcattg atagagttat
      121 tttaccactc cctatcagtg atagagaaaa gtgaaatgaa tagttcgaca aagatcgcat
      181 tggtaattac gttactcgat gccatgggga ttggccttat catgccagtc ttgccaacgt
      241 tattacgtga atttattgct tcggaagata tcgctaacca ctttggcgta ttgcttgcac
      301 tttatgcgtt aatgcaggtt atctttgctc cttggcttgg aaaaatgtct gaccgatttg
      361 gtcggcgccc agtgctgttg ttgtcattaa taggcgcatc gctggattac ttattgctgg
      421 ctttttcaag tgcgctttgg atgctgtatt taggccgttt gctttcaggg atcacaggag
      481 ctactggggc tgtcgcggca tcggtcattg ccgataccac ctcagcttct caacgcgtga
      541 agtggttcgg ttggttaggg gcaagttttg ggcttggttt aatagcgggg cctattattg
      601 gtggttttgc aggagagatt tcaccgcata gtcccttttt tatcgctgcg ttgctaaata
      661 ttgtcgcttt ccttgtggtt atgttttggt tccgtgaaac caaaaataca cgtgataata
      721 cagataccga agtaggggtt gagacgcaat cgaattcggt atacatcact ttatttaaaa
      781 cgatgcccat tttgttgatt atttattttt cagcgcaatt gataggccaa attcccgcaa
      841 cggtgtgggt gctatttacc gaaaatcgtt ttggatggaa tagcatgatg gttggctttt
      901 cattagcggg tcttggtctt ttacactcag tattccaagc ctttgtggca ggaagaatag
      961 ccactaaatg gggcgaaaaa acggcagtac tgctcggatt tattgcagat agtagtgcat
     1021 ttgccttttt agcgtttata tctgaaggtt ggttagtttt ccctgtttta attttattgg
     1081 ctggtggtgg gatcgcttta cctgcattac agggagtgat gtctatccaa acaaagagtc
     1141 atcagcaagg tgctttacag ggattattgg tgagccttac caatgcaacc ggtgttattg
     1201 gcccattact gtttgctgtt atttataatc attcactacc aatttgggat ggctggattt
     1261 ggattattgg tttagcgttt tactgtatta ttatcctgct atcgatgacc ttcatgttaa
     1321 cccctcaagc tcaggggagt aaacaggaga caagtgctta gttatttcgt caccaaatga
     1381 tgttattccg cgaaatataa tgaccctctt gataacccaa gagggcattt tttacgataa
     1441 agaagattta gcttcaaata aaacctatct attttattta tctttcaagc tcaataaaaa
     1501 gccgcggtaa atagcaataa attggccttt tttatcggca agctctttta ggtttttcgc
     1561 atgtattgcg atatgcataa accagccatt gagtaagttt ttaagcacat catcatcata
     1621 agctttaagt tggttctctt ggatcaattt gctgacaatg gcgtttacct taccagtaat
     1681 gtattcaagg ctaatttttt caagttcatt ccaaccaatg ataggcatca cttcttggat
     1741 agggataagg tttttattat tatcaataat ataatcaaga taatgttcaa atatactttc
     1801 taaggcagac caaccatttg ttaaatcagt ttttgttgtg atgtaggcat caatcataat
     1861 taattgctgc ttataacagg cactgagtaa ttgtttttta tttttaaagt gatgataaaa
     1921 ggcacctttg gtcaccaacg cttttcccga gatctcatct attgaaacag cttgatagcc
     1981 tttttcaaca aacaatattc gtgctgagtt aaccagtgat tgataggtac tcttaaaatt
     2041 ttcttgttga tgatttttat tttccatgat agatttaaaa taacataccg tcagtatgtt
     2101 tatggtatca tgatgatgtg gtcgtgacaa tcttaagaac atttaggtta ttttatgtat
     2161 attgaacagc attctcgcta tcaaaataaa gctaataaca tccaattaag atatgatgat
     2221 aagcagtttc atacaacggt tatcaaagat gttctattat ggattgaaca taatttagat
     2281 cagtctttac tgcttgatga tgtggcgaat aaagcgggtt ataccaagtg gtattttcag
     2341 cggctgttca aaaaagtaac aggggtcaca ctggctagct atattcgtgc tcgtcgtttg
     2401 acgaaagcgg ctgttgagtt gaggttgacg aa
//
LOCUS       DAJTQQ010000393         1915 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.guided.242, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000393 DAJTQQ010000000
VERSION     DAJTQQ010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.guided.242"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            complement(1..775)
                     /gene="intI2"
                     /locus_tag="OO309_004462"
                     /pseudo
     CDS             complement(1..775)
                     /gene="intI2"
                     /locus_tag="OO309_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            1107..1580
                     /locus_tag="OO309_004463"
     CDS             1107..1580
                     /locus_tag="OO309_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5849311.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1675..>1915
                     /locus_tag="OO309_004464"
     CDS             1675..>1915
                     /locus_tag="OO309_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat1"
                     /protein_id="HCR5849312.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTW"
BASE COUNT          552 a          390 c          433 g          540 t
ORIGIN      
        1 cgatgcctgc tttttgtacg gctgccttca atgcctttcg cgcaacggag tcatgcagat
       61 gatggcggca taatttgccg ttatacgggt ggttgcagag cgtgctggag ggaaagacaa
      121 acatccacgc cgcttgtcga taagcagaag ggtatttgtg atctaaagca aaaggcagcg
      181 atggccctac gccttgtaag ttgtcgtctt gctgaataag ccgcgcttgc tcaatgagtt
      241 attttattgc tgggattagg cgcgtgggca gtaggctgtt tctgcttttc ccacccttac
      301 cgtcatgcac agtgatgcag ccattatcaa aatcaaaatc tttaacccgc aaacgcaagc
      361 attcattaat gcgcaaacct gcaccataca gcagcgtaaa aataacttgg ttgcgagtat
      421 ccataacctg caaaatgcgt tgcacttcat ttgcagagat aacagagggt agccgtctag
      481 gcttgcttgc agggatataa tcaatatcgc ccaacggctg ttgtaaaaac ctgttgtaca
      541 aaaaagctag ggcatttaaa gcgattttct gcgtgtttat ggctacatgt ctgctgtttg
      601 ctaagctgga taaaaacagc ctgacctctt cactgcccat ggtctgagga tgacgttttt
      661 tgtgaaacag aataaaacgc ttaatccagt gcaggtaagt tttttcagtt ttcagcgcat
      721 aacctttttg tcgcatatcc gtgcgtatag aatttaaaaa tggactgtta gacataaaac
      781 gctccttgtc ttgcaactgt ctgcctatac agcctattct agctgggatt taaaaaagtg
      841 cctgtttttt acgcctagag atgcttgttt accggtagag ttttaattta atgctaaata
      901 aattaaaatg ttatgagttc tttgggtgag ataatgtgca tcgtgcaagc aggatagacg
      961 gcatgcacga tttgtaataa cagagtgtct tgtattttta aagaaagtct atttaataca
     1021 agtgattata ttaattaacg gtaagcatca gcgggtgaca aaacgagcat gcttactaat
     1081 aaaatgttaa cctctgagga agaattgtga aactatcact aatggtagct atatcgaaga
     1141 atggagttat cgggaatggc cctgatattc catggagtgc caaaggtgaa cagctcctgt
     1201 ttaaagctat tacctataac caatggctgt tggttggacg caagactttt gaatcaatgg
     1261 gagcattacc caaccgaaag tatgcggtcg taacacgttc aagttttaca tctgacaatg
     1321 agaacgtatt gatctttcca tcaattaaag atgctttaac caacctaaag aaaataacgg
     1381 atcatgtcat tgtttcaggt ggtggggaga tatacaaaag cctgatcgat caagtagata
     1441 cactacatat atctacaata gacatcgagc cggaaggtga tgtttacttt cctgaaatcc
     1501 ccagcaattt taggccagtt tttacccaag acttcgcctc taacataaat tatagttacc
     1561 aaatctggca aaagggttaa caagtggcag caacggattc gcaaacctgt cacgcctttt
     1621 gtaccaaaag ccgcgccagg tttgcgatcc gctgtaccag gcgttaggcg tcatatgaag
     1681 atttcggtga tccctgagca ggtggcggaa acattggatg ctgagaacca tttcattgtt
     1741 cgtgaagtgt tcgatgtgca cctatccgac caaggctttg aactatctac cagaagtgtg
     1801 agcccctacc ggaaggatta catctcggat gatgactctg atgaagactc tgcttgctat
     1861 ggcgcattca tcgaccaaga gcttgtcggg aagattgaac tcaactcaac atgga
//
LOCUS       DAJTQQ010000394         1192 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.guided.271, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000394 DAJTQQ010000000
VERSION     DAJTQQ010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.guided.271"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..283
                     /locus_tag="OO309_004465"
     CDS             <1..283
                     /locus_tag="OO309_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5849313.1"
                     /translation="DLASIEHIVVSHTHRGKGVAHSLIEFAKKWALSRQLLGIRLETQ
                     TNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNETAMYWYWFSGAQDDA"
     gene            341..1132
                     /gene="aadA1"
                     /locus_tag="OO309_004466"
     CDS             341..1132
                     /gene="aadA1"
                     /locus_tag="OO309_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5849314.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          305 a          301 c          319 g          267 t
ORIGIN      
        1 cgatctagcc tctatcgaac acattgttgt gtcgcacacg caccgaggca aaggagtcgc
       61 gcacagtctc atcgaatttg cgaaaaagtg ggcactaagc agacagctcc ttggcatacg
      121 attagagaca caaacgaaca atgtacctgc ctgcaatttg tacgcaaaat gtggctttac
      181 tctcggcggc attgacctgt tcacgtataa aactagacct caagtctcga acgaaacagc
      241 gatgtactgg tactggttct cgggagcaca ggatgacgcc taacaattca ttcaagccga
      301 caccgcttcg cggcgcggct taattcagga gttaaacatc atgagggaag cggtgatcgc
      361 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
      421 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga
      481 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
      541 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
      601 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
      661 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
      721 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
      781 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
      841 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
      901 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
      961 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     1021 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     1081 agaatttgtt cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctaac
     1141 aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta ga
//
LOCUS       DAJTQQ010000395         1190 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.guided.273, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000395 DAJTQQ010000000
VERSION     DAJTQQ010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.guided.273"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            264..923
                     /locus_tag="OO309_004467"
     CDS             264..923
                     /locus_tag="OO309_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5849315.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT          321 a          257 c          279 g          333 t
ORIGIN      
        1 acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat
       61 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
      121 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      181 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      241 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      301 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      361 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      421 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      481 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      541 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      601 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      661 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
      721 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
      781 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
      841 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
      901 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
      961 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
     1021 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
     1081 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
     1141 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt
//
LOCUS       DAJTQQ010000396         1189 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.guided.274, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000396 DAJTQQ010000000
VERSION     DAJTQQ010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.guided.274"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..283
                     /locus_tag="OO309_004468"
     CDS             <1..283
                     /locus_tag="OO309_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5849316.1"
                     /translation="DLASIEHIVVSHTHRGKGVAHSLIEFAKKWALSRQLLGIRLETQ
                     TNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNETAMYWYWFSGAQDDA"
     gene            341..1129
                     /gene="aadA1"
                     /locus_tag="OO309_004469"
     CDS             341..1129
                     /gene="aadA1"
                     /locus_tag="OO309_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5849317.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          302 a          301 c          319 g          267 t
ORIGIN      
        1 cgatctagcc tctatcgaac acattgttgt gtcgcacacg caccgaggca aaggagtcgc
       61 gcacagtctc atcgaatttg cgaaaaagtg ggcactaagc agacagctcc ttggcatacg
      121 attagagaca caaacgaaca atgtacctgc ctgcaatttg tacgcaaaat gtggctttac
      181 tctcggcggc attgacctgt tcacgtataa aactagacct caagtctcga acgaaacagc
      241 gatgtactgg tactggttct cgggagcaca ggatgacgcc taacaattca ttcaagccga
      301 caccgcttcg cggcgcggct taattcagga gttaaacatc atgagggaag cggtgatcgc
      361 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
      421 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga
      481 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
      541 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
      601 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
      661 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
      721 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
      781 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
      841 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
      901 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
      961 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     1021 taggcaggct tatcttggac aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     1081 atttgttcac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctaacaat
     1141 tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca agcgttaga
//
LOCUS       DAJTQQ010000397         1067 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.guided.281, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000397 DAJTQQ010000000
VERSION     DAJTQQ010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.guided.281"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..340
                     /locus_tag="OO309_004470"
     CDS             <1..340
                     /locus_tag="OO309_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR5849318.1"
                     /translation="KTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKK
                     ITDHVIVSGGGEIYKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNI
                     NYSYQIWQKG"
     gene            435..959
                     /gene="sat2"
                     /locus_tag="OO309_004471"
     CDS             435..959
                     /gene="sat2"
                     /locus_tag="OO309_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5849319.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
BASE COUNT          314 a          247 c          244 g          262 t
ORIGIN      
        1 caagactttt gaatcaatgg gagcattacc caaccgaaag tatgcggtcg taacacgttc
       61 aagttttaca tctgacaatg agaacgtatt gatctttcca tcaattaaag atgctttaac
      121 caacctaaag aaaataacgg atcatgtcat tgtttcaggt ggtggggaga tatacaaaag
      181 cctgatcgat caagtagata cactacatat atctacaata gacatcgagc cggaaggtga
      241 tgtttacttt cctgaaatcc ccagcaattt taggccagtt tttacccaag acttcgcctc
      301 taacataaat tatagttacc aaatctggca aaagggttaa caagtggcag caacggattc
      361 gcaaacctgt cacgcctttt gtaccaaaag ccgcgccagg tttgcgatcc gctgtaccag
      421 gcgttaggcg tcatatgaag atttcggtga tccctgagca ggtggcggaa acattggatg
      481 ctgagaacca tttcattgtt cgtgaagtgt tcgatgtgca cctatccgac caaggctttg
      541 aactatctac cagaagtgtg agcccctacc ggaaggatta catctcggat gatgactctg
      601 atgaagactc tgcttgctat ggcgcattca tcgaccaaga gcttgtcggg aagattgaac
      661 tcaactcaac atggaacgat ctagcctcta tcgaacacat tgttgtgtcg cacacgcacc
      721 gaggcaaagg agtcgcgcac agtctcatcg aatttgcgaa aaagtgggca ctaagcagac
      781 agctccttgg catacgatta gagacacaaa cgaacaatgt acctgcctgc aatttgtacg
      841 caaaatgtgg ctttactctc ggcggcattg acctgttcac gtataaaact agacctcaag
      901 tctcgaacga aacagcgatg tactggtact ggttctcggg agcacaggat gacgcctaac
      961 aattcattca agccgacacc gcttcgcggc gcggcttaat tcaggagtta aacatcatga
     1021 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagtt
//
LOCUS       DAJTQQ010000398         1067 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.guided.282, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000398 DAJTQQ010000000
VERSION     DAJTQQ010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.guided.282"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            <1..340
                     /locus_tag="OO309_004472"
     CDS             <1..340
                     /locus_tag="OO309_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCR5849320.1"
                     /translation="KTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKK
                     ITDHVIVSGGGEIYKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNI
                     NYSYQIWQKG"
     gene            435..959
                     /gene="sat2"
                     /locus_tag="OO309_004473"
     CDS             435..959
                     /gene="sat2"
                     /locus_tag="OO309_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5849321.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWSDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
BASE COUNT          313 a          247 c          245 g          262 t
ORIGIN      
        1 caagactttt gaatcaatgg gagcattacc caaccgaaag tatgcggtcg taacacgttc
       61 aagttttaca tctgacaatg agaacgtatt gatctttcca tcaattaaag atgctttaac
      121 caacctaaag aaaataacgg atcatgtcat tgtttcaggt ggtggggaga tatacaaaag
      181 cctgatcgat caagtagata cactacatat atctacaata gacatcgagc cggaaggtga
      241 tgtttacttt cctgaaatcc ccagcaattt taggccagtt tttacccaag acttcgcctc
      301 taacataaat tatagttacc aaatctggca aaagggttaa caagtggcag caacggattc
      361 gcaaacctgt cacgcctttt gtaccaaaag ccgcgccagg tttgcgatcc gctgtaccag
      421 gcgttaggcg tcatatgaag atttcggtga tccctgagca ggtggcggaa acattggatg
      481 ctgagaacca tttcattgtt cgtgaagtgt tcgatgtgca cctatccgac caaggctttg
      541 aactatctac cagaagtgtg agcccctacc ggaaggatta catctcggat gatgactctg
      601 atgaagactc tgcttgctat ggcgcattca tcgaccaaga gcttgtcggg aagattgaac
      661 tcaactcaac atggagcgat ctagcctcta tcgaacacat tgttgtgtcg cacacgcacc
      721 gaggcaaagg agtcgcgcac agtctcatcg aatttgcgaa aaagtgggca ctaagcagac
      781 agctccttgg catacgatta gagacacaaa cgaacaatgt acctgcctgc aatttgtacg
      841 caaaatgtgg ctttactctc ggcggcattg acctgttcac gtataaaact agacctcaag
      901 tctcgaacga aacagcgatg tactggtact ggttctcggg agcacaggat gacgcctaac
      961 aattcattca agccgacacc gcttcgcggc gcggcttaat tcaggagtta aacatcatga
     1021 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagtt
//
LOCUS       DAJTQQ010000399          993 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.guided.290, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000399 DAJTQQ010000000
VERSION     DAJTQQ010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.guided.290"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            142..933
                     /gene="aadA1"
                     /locus_tag="OO309_004474"
     CDS             142..933
                     /gene="aadA1"
                     /locus_tag="OO309_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5849322.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          252 a          254 c          261 g          226 t
ORIGIN      
        1 tcaccattct aaacacacta aatttataaa aaatctaatg gcaaaatcgc ccaacccttc
       61 aatcaagtcg ggacggccaa aagcaagctt ttggctcccc tcgctggcgc tcggcgcccc
      121 ttatttcaaa cgttaaacat catgagggaa gtggtgatcg ccgaagtatc gactcaacta
      181 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
      241 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
      301 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
      361 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
      421 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
      481 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
      541 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
      601 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
      661 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
      721 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
      781 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
      841 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt tcactacgtg
      901 aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa caattcgttc aagccgacgc
      961 cgcttcgcgg cgcggcttaa ctcaagcgtt aga
//
LOCUS       DAJTQQ010000400          990 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 603867
            SAMEA8900772-rid15818933.guided.292, whole genome shotgun sequence.
ACCESSION   DAJTQQ010000400 DAJTQQ010000000
VERSION     DAJTQQ010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900772
            Sequence Read Archive: ERR6005214
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478128.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478128.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:31:58
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,475
            CDSs (total)                      :: 4,396
            Genes (coding)                    :: 4,008
            CDSs (with protein)               :: 4,008
            Genes (RNA)                       :: 79
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 3 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 388
            CDSs (without protein)            :: 388
            Pseudo Genes (ambiguous residues) :: 0 of 388
            Pseudo Genes (frameshifted)       :: 185 of 388
            Pseudo Genes (incomplete)         :: 101 of 388
            Pseudo Genes (internal stop)      :: 145 of 388
            Pseudo Genes (multiple problems)  :: 41 of 388
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900772-rid15818933.guided.292"
                     /serovar="S. flexneri 2b"
                     /isolate="603867"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2010-05-19"
     gene            142..930
                     /gene="aadA1"
                     /locus_tag="OO309_004475"
     CDS             142..930
                     /gene="aadA1"
                     /locus_tag="OO309_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5849323.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          249 a          254 c          261 g          226 t
ORIGIN      
        1 tcaccattct aaacacacta aatttataaa aaatctaatg gcaaaatcgc ccaacccttc
       61 aatcaagtcg ggacggccaa aagcaagctt ttggctcccc tcgctggcgc tcggcgcccc
      121 ttatttcaaa cgttaaacat catgagggaa gtggtgatcg ccgaagtatc gactcaacta
      181 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
      241 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
      301 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
      361 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
      421 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
      481 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
      541 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
      601 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
      661 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
      721 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
      781 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctaggcaggc ttatcttgga
      841 caagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgttca ctacgtgaaa
      901 ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc
      961 ttcgcggcgc ggcttaactc aagcgttaga
//
